LOCUS       DAOQYR010000001        60806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_524_length_60806_cov_9.145726, whole genome shotgun sequence.
ACCESSION   DAOQYR010000001 DAOQYR010000000
VERSION     DAOQYR010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 60806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60806
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_524_length_60806_cov_9.145726"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PKM87_00005"
     CDS             <1..803
                     /locus_tag="PKM87_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F420-dependent methylenetetrahydromethanopterin
                     dehydrogenase"
                     /protein_id="HNQ28481.1"
                     /translation="ASGVMAELLLDERADREDMQTFMATSGTKLQPEDIDRVVNNMVA
                     WKPDFAIVVSPNGVLPGPTGARERLLASGIPTVFITDDVTTKKEWAEIKDGKFGYIIM
                     KGDAMIGARREFLDPIEMADYNGNLVKVLSVTGAFRKLQMALDQVIDQVKAGKKGAEI
                     ALPKLVVTSDKAVDGEFTNPYALAKARAAYEIASAVAMVNVKGCFMTKEWEKYIPIVC
                     SAHEMMRVAAVLCDEARELEKACDGVIRKPHKKDGVIVAKTKLISKPE"
     gene            complement(910..1839)
                     /locus_tag="PKM87_00010"
     CDS             complement(910..1839)
                     /locus_tag="PKM87_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNQ28482.1"
                     /translation="MFTSTTVRSGDHVIEYRQEQISGIRCKISPERLNRRLDVAYVPP
                     TLIPPCPFCPGQVEEVTPCFPGGTRIVKGESLTFPNLFPYAEMHTVTVITRAHHVEEF
                     KPRQVSDALEGMVESLEEHEGYASLNWNYLPSAGASIAHPHLQGLVDRRPPALAERYL
                     EGGFRYLLEHGHSYWDDFLCAERTGPRYLFGDEVCWLAQAVPLGEREVRAILPISCMN
                     DFEPYIETFVKGLLSILSMYRSLGTHAFNMSLFFDRPGASRGFSAFCSVISRINPNAS
                     STSDSAFMERLHQEPVILTLPEELSAYFSKSRI"
     gene            complement(1927..2916)
                     /locus_tag="PKM87_00015"
     CDS             complement(1927..2916)
                     /locus_tag="PKM87_00015"
                     /EC_number="1.5.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5,10-methylenetetrahydromethanopterin reductase"
                     /protein_id="HNQ28483.1"
                     /translation="MTTYGIEFVPGNVNVKQVVNFCKLAESKDIDYAWITNHYNNRHC
                     YPTLAMIAAATTTLKMGPGIMNSFTDTPAAMASFACTLNEISDGRAVLGIGPGDLSTL
                     PKLAISPEKPVARLGEAIAQIRKLCAGEEVKKSGMQFFDYDGAKLTGVNLPGKKGIPI
                     YVGAQGPKVLELAGTVGDGALINASNPKDFAVAIPIIKAACDKVGKKGFDVGAYTAMS
                     IDTNEKKARNAAKIVAAFIAAGSPPDLLQRHGLDAAKVANIKNALAKFDFKTAGENVD
                     DKTIDAFTIAGTPDTVKQKCEDLAKAGVTQIIFGSPLGPDMTNSIRLLGKYIV"
     gene            3397..4584
                     /locus_tag="PKM87_00020"
     CDS             3397..4584
                     /locus_tag="PKM87_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin/FtsZ family protein"
                     /protein_id="HNQ28484.1"
                     /translation="MRILTIGLGGAGCRIVDRLLAHDQKAPVRCVFGIAIDCDAGQLN
                     SLQHVPVDHRLFFQPLDPALSGDLAAHLPSEEALSRLQALDDGDIDALFIYTGLGGTM
                     AGTAPGLVSYLKERLAEPVFCLCTLPCLQEGDERCSAAADHLDTLHGLLDGIVLFDNE
                     SWRGRVFPAPDEPGISRAGDIGALVRRRTEPPPPVSETELFYRNFNDIISRRFTLLLR
                     AGEYSERAPGDLPEVVLDAGEILNTIQGMGFITMGYAREEVPSGSPMDLLSRFRPGTP
                     AVQDGHLKASRVVELAKKAIFEEISAPTALEEAKKALILIAGPSQEMSMRGFMTVRRW
                     IDRTIRGLELRSGDYPVSDTRFLGIFIILAGMESLPAVDMLRQIRDRMRGMQAEPEES
                     PGT"
     gene            complement(4648..5595)
                     /locus_tag="PKM87_00025"
     CDS             complement(4648..5595)
                     /locus_tag="PKM87_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HNQ28485.1"
                     /translation="MIRTERKYIEILRILKEHREPMGAKRLSELMAERGFVLSDRAVQ
                     YYLRYLDEMGFTEKVGNMGRILTPYGKEETESALVGDRIGFIISKLERLAFRSTFDPE
                     TGTGDVAYNLSIVPEERLPDAMKAFNEVIAAKCGFFSSYRVIDRDPRIPPGSAGIMSI
                     CSITMDGVFQRRGIPVRMAYGGRLDIQHGEATRFVDLIGYRGTTIDPLQLFISAGLTS
                     LSSLVSTGTGTGLANVREIPALAEGRAREVVAQMKKAGFVFPVAMGTSVFNLVPDPYR
                     LAIVAFSGMNFVANTVEKGVPIRTEIGAGNVPFSKIHHE"
     gene            complement(5596..6033)
                     /gene="dcd"
                     /locus_tag="PKM87_00030"
     CDS             complement(5596..6033)
                     /gene="dcd"
                     /locus_tag="PKM87_00030"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNQ28486.1"
                     /translation="MILSSSEIERRLRGEDMSGDLVIEPYSEECQQPASYDLRVGGDF
                     VLPPRVCTLVNTLERVELPSDLAATMRCRSSFGRRGVILTAGFVDPGFRGQLTLCLVN
                     MGAEHLTLRKNDRVVQIIFHQVTDGKNAYSGRYQDSTGVIKAR"
     gene            6092..6163
                     /locus_tag="PKM87_00035"
     tRNA            6092..6163
                     /locus_tag="PKM87_00035"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6125..6127,aa:Arg,seq:tcg)
     gene            complement(6245..7636)
                     /locus_tag="PKM87_00040"
     CDS             complement(6245..7636)
                     /locus_tag="PKM87_00040"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNQ28487.1"
                     /translation="MQRIGTCFAFALLAMLLMAGPASGVTVLVNIYEKEGNITPLPGA
                     ALYTNNALVGKSDSGGNIEFSHPGTEIIQIRVEKLGYAPWSGDIGLNATTLLVEMTKK
                     KVPLTVQVFDTDALAPVAGASVSISDGAGKNTTVTDANGTTMFTVSGDSAYNLEITAP
                     NYRTFTDHLEIARDQKTVQVMLVRDDRFSVLVKDETSGAPLPGAAVIIDGVERGITDA
                     KGVVTLSLPREKVYQVHVRIEGYDDYHERMNVGRDQALVTTLLRKSPYSVFISVYNEE
                     RDPVEEASVFLDGQPVGSTNRYGRLQLANLSFGEYALEVRHPGFETHRQQMQVRVQGE
                     DITVDLAFPAANLSIAALEGGDVPVHGATIFIDGKEQGTTGENGTLPVRLRLNTPYVI
                     SAEKEGFNPVKLEKTVSSRNETAPVIISMERSIDWVLVIVVLAVVVAGFMGAFLIMKK
                     RGQARGRSRRGGL"
     gene            complement(7799..9325)
                     /locus_tag="PKM87_00045"
     CDS             complement(7799..9325)
                     /locus_tag="PKM87_00045"
                     /EC_number="2.4.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP phosphorylase"
                     /protein_id="HNQ28488.1"
                     /translation="MKLTVKLVDIGTREVLLHIDDSRNIGVLPGDRIQILNEVTGVSV
                     AAFIDTTTTLLPKGTIGIYQVTNERLQLEDGVSVEVRKAERPVTLDYIKKKMDGQHLT
                     KDETYAIIRDVISDNLSPAELTAYITACYINEMEMDEVEHLTRAMVDTGERLTFHAHP
                     IVDKHSIGGVPGNKISLLVVPIIAASGLKIPKTSSRAITGAGGTADLMEVLAPVDFSA
                     SEVQRMTEKVGGVIVWGGATNIAPADDKIIIQEYPFRIDARGQMLASVMAKKYAVGAD
                     LVVIDIPVGTHAKVATIQEGRKLAREFIELGDRLNMQVECALTYGDSPVGRSIGPNLE
                     VMEALRILEGGESPGSLLQKSVAIAGMALEMSGKAARGMGAQMAEEILKKGDALKKFR
                     QIIEIQGGDPKIRSDDIEPGKYQFVVNAPTSGYVIELNNRSLISLARTAGAPHDRGAG
                     VLLHAKKGSRVEKGQPLFTIYADRNWRLQKALEEGRQLMPVLVEGMLLDRVPHDTRWS
                     "
     gene            complement(9550..12087)
                     /locus_tag="PKM87_00050"
     CDS             complement(9550..12087)
                     /locus_tag="PKM87_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEMAR_RS02690 family S-layer glycoprotein"
                     /protein_id="HNQ28489.1"
                     /translation="MIKRFSLALIALVALAVLMLPASAATIVSVPAGGDVFIGEEGLD
                     ISAGLGAATQIAYWASGTSTGDQPTNIVTVGTPTSYYVSPQIFVGKVGTWYQWTGVRG
                     AAVFNVVDPALTVKVWDNTAGKDVTGKSVPPGNYLNFRVETNAYSVTARADAAGKGFV
                     NIRVKTADGATYTALFQTTTTSRPLTGLAPNSDLWYWAPQVTNQGWNTDVLDANGARM
                     YKAGTYTVYAEFDLNKIKDNYKAPDGTDYTGKTISSTQTVIIASDTVKIEASKDVVVR
                     GNPFSVTITGRPNTYYVLWAKGTGQMSGAALDQPPLLVASQKDVDTSAAIGQYRYEGG
                     AGRTVAQDVPATPANSYFAAAKTSNSGTITVGWVTSQATKDKKYTFRVERGPGAAGIP
                     APWDTLLYADQYKSDEVDVKVEKGTVTVVAAGDQSYFLGQEVKLTGTNSETDTVYLFI
                     TGPNLPSQGGRLTDPRTGVVNGVTTSFTTADVLDDNTWEYKWQTANLNIDAGTYTIYA
                     VATPSDKNTLADTQYATVSIIIRKPFVTAVASQSTIAQGDKFYIRGVAEGQPSQGVQV
                     WIMGKNYVRVDTESVNNDGSFEKEINQGITANMAPGQYFVVVQHPMYNDQLDVNPRAV
                     APWPYREVVGPYPVANSVLFLLEGPGSLQGSDAAEALVVALDNAAVDDTYTKLQFLIE
                     APTIKITPITEKQVGEKFEIKGTTNLAVDDEILVEVVSSSFSPTQKTQSGEFSGATGT
                     VKVQKGTEGFNTWSFPVDTTTFKPDEYIVQVSGVTVDVTSTALFNVVEVKPTTIPTTA
                     PPTQVTTAATPVPTTATPTPTPTTPGFGALVALIGLGAVAFLIVRKH"
     gene            12549..13262
                     /locus_tag="PKM87_00055"
     CDS             12549..13262
                     /locus_tag="PKM87_00055"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNQ28490.1"
                     /translation="MRIIKAPTLASAHEQVVRMILEKGWTLDTEDREATVEFEEVAMR
                     VEQPGNPPMVSPCSRFQQRFMEQYARDLIEGSDSVFEYDYHDRLFNWGGGLMRGGGNE
                     VHVDQVAYIVRKLREAPASRRAIAITWNPAVDENLDDCPCLQLVQCVSRDGMLHMKVV
                     FRSNDMLTAAGANMFALVSLQQMIARSLELGCGSYTHISLVPHIYYRRDADDIEPFCG
                     HGERFRPIPEVCRACGKCP"
     gene            13347..13419
                     /locus_tag="PKM87_00060"
     tRNA            13347..13419
                     /locus_tag="PKM87_00060"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13381..13383,aa:Gln,seq:ctg)
     gene            complement(13442..14044)
                     /locus_tag="PKM87_00065"
     CDS             complement(13442..14044)
                     /locus_tag="PKM87_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrillarin-like rRNA/tRNA
                     2'-O-methyltransferase"
                     /protein_id="HNQ28491.1"
                     /translation="MIWVEGVLVSEGEGGVYGERTIGGQRVWNPYRSKLAAYYYRGGD
                     IDIEDSMRVLYLGAANGTTVSHVADYAETVYAVENAYAPMRDLLAVAARRKNIIPLFA
                     DATKPHEYAPLVETVQMLFQDVAHPDQAGIALWNSLFLDTGGAAVIAIKTRSIDVSRS
                     PREVVDDFCSALEGSPLAVKDVIWLEPYHRDHAILVCRKQ"
     gene            complement(14041..>14643)
                     /locus_tag="PKM87_00070"
     CDS             complement(14041..>14643)
                     /locus_tag="PKM87_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-processing protein"
                     /protein_id="HNQ28492.1"
                     /translation="VHMVRMLDQIDEAINLLTGRALEWHAAKDPSFSRKYRELQGRRA
                     RELLAGSKNPVLVAVATETAHLAEVRTALSRDVAALAEKVMPNSSVLVGGVVAARLLS
                     AAGGLPRLARLPAATIQVMGARLALFSHLKSGTPPPKHGIIYQHRRVHAAGKGRRGKV
                     SRALAAKLAIAARIDFYRGTADPVFLESAERAIDRAGDRK"
     assembly_gap    14644..14743
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14744..15056)
                     /locus_tag="PKM87_00075"
     CDS             complement(<14744..15056)
                     /locus_tag="PKM87_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-processing protein"
                     /protein_id="HNQ28493.1"
                     /translation="MVSYWFGDVDGGVCTPCPSREPDFLSDRLDRIIQTRERHTPFSW
                     EVARECGFVSSRGEYVALLRSLALLRVEKELRRVSQLPEMELVHMVRMLDQIDEAINL
                     LT"
     gene            complement(15122..16333)
                     /locus_tag="PKM87_00080"
     CDS             complement(15122..16333)
                     /locus_tag="PKM87_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HNQ28494.1"
                     /translation="MIEILAGTAAGVALGLLSGLLPGIHVNTLAAIMLASLAVLLPLF
                     GPFFLAAALFSALVTHCFIDAVPSTFLGIPDADTALAVLPAHALCMEGRGEEAVRISA
                     LGSATGLALAVPLTCILLLVIPHFQSVIDWASGIILVAVIGFLLVQSEAPGWGLLVFC
                     TSGLLGIFTFRYSFLAPPLLGESAVLMPLLSGLFGISMLIYSGKGAIPPQRFSGLDIS
                     REGILKGTVLGGAAGLVVGWLPGLSNATANGVLASAVNYTRDRRGYLLATSAANTMNA
                     VVGLAAFFAVGRMRNGVVAALALLEIPPLSFILFSGALAALAAYLLTIRLAGMAHLLE
                     GVDRTWLNRVVMAFVAVVCFLGAGLFGLFLLVMATAVGCIPRLVNIPQVFCMGAIMVP
                     VMFYSFGLGVI"
     gene            16416..17123
                     /locus_tag="PKM87_00085"
     CDS             16416..17123
                     /locus_tag="PKM87_00085"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNQ28495.1"
                     /translation="MLIAITRLQEKSTDDQARCARFGHRCFPVSPLQARINQEEIAWF
                     VGETDRNTFDCIFFTSALPARLIGPRLSRWPRVVAIGPQTRRELEAIGIEAEVLPRYY
                     SRDFVDYLGPWIEGKHIGIPRADVPNPGLLSSIRDAGGKATEVACYSLIPTGERLSLG
                     DAEVLLFTSAMSYRKARWENRAGLILAAIGDITAGAMRDGGHHPSVVGDGSLEGTLEA
                     LNHYLDTHPRPERTGRR"
     gene            17120..18034
                     /locus_tag="PKM87_00090"
     CDS             17120..18034
                     /locus_tag="PKM87_00090"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided pseudouridylation complex
                     pseudouridine synthase subunit Cbf5"
                     /protein_id="HNQ28496.1"
                     /translation="MTPFPVPEHGLLVLDKPRGPSSHQVTAWAGEILGKRVGHAGTLD
                     PGVSGVLVVMVGNAVRLAPILLAEEKEYVAVIRTHGDVQEDALRSVASDFCGRIYQRP
                     PRRSAVARNLRIRTVRALEVREFHERLAVLGITCDAGTYIRSLCHHIGLALGCGAHMQ
                     ELRRVRSGIFSEEQAHTLHDLKDAATLAGQGDPQYLAGMILPPEKVARGLPTLVVRDT
                     AVDALCHGAALAGVGVVRQDAFARGEMVAVLTGKGELVCIGEAIVSSTQVPPGSTGLV
                     AAPRSVFMRPGTYPKGWKKREGAEKPEI"
     gene            18150..18233
                     /locus_tag="PKM87_00095"
     tRNA            18150..18233
                     /locus_tag="PKM87_00095"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18182..18184,aa:Ser,seq:gga)
     gene            complement(18228..19001)
                     /locus_tag="PKM87_00100"
     CDS             complement(18228..19001)
                     /locus_tag="PKM87_00100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNQ28497.1"
                     /translation="MSPHDLGPFSPDTIYHGDALALLPLLRGGSIDLIVTDPPFAIDF
                     RARKMNYNRTGSRVIEGYREIPPGEYGAFTSRWISEAFRVLSPTGSLYVFSGWNRLKE
                     VLCSLDEAGFSTINHLIWKYQFGVFTRRKYVTSHYHILFAVKDPGHYTFHKVEHYPED
                     VWVINREYWKGKKKTPTKLPPQLVRKILSFSSSPGDLVLDPFLGSGTVAAVSQEMGRR
                     FLGFEVVDEYYRFALQRLEEGQSLPGQGRGSTDGRTKKR"
     gene            complement(18998..19603)
                     /locus_tag="PKM87_00105"
     CDS             complement(18998..19603)
                     /locus_tag="PKM87_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HNQ28498.1"
                     /translation="MSGSYDVLMVGIGGQGIILASNILGEACLIEGRNVRGAETHGMA
                     QRGGSVEGHIRIDGKFGPLIAPGTAQLMIAFDMLEALRYSHFLAPEGHMVVNSHIVIP
                     TSVFMQGAAVPEKEAVQERLKSRALCLVDADALAVEAGSPLSQNVVMIGAASWFMPFA
                     PASLLQAVERLVPKKTVEINRKAFELGRAAGERCREGSSDT"
     gene            complement(19600..21369)
                     /gene="iorA"
                     /locus_tag="PKM87_00110"
     CDS             complement(19600..21369)
                     /gene="iorA"
                     /locus_tag="PKM87_00110"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HNQ28499.1"
                     /translation="MTTQYLLGNEAIVKGCLESPVHFVTGYPGTPSSEVIDILRSLPE
                     RDFYVEWSVNEKVAFENALAAAWCGLRALCTMKHVGLNVAADPLMTSAYTGVTGGLVV
                     MSADDPFAHSSQNEQDSRCYALFSRLPCLDPSTIQEAHDMVRDAFALSEEFSLPVIFR
                     PTTRICHSKGDTLVRSPASVPRKGEFHKNPQQYVVIPAHTRVLHRKLNQKQPHIKRAL
                     VEKGYNHAEVRGSVAVVASGIAAAYVREVLPPDVSFMKIGAYPIDEEWLGGFVRKHES
                     VLVIEELHPVVEEAVRQVSGGVAVHGKKDGMVPYEGELSPVIVARVMQACGIAPTREF
                     PNPVPAGDIPNRPPILCAGCMHRPVFYAMRKVFKDGIFPSDIGCYTLGLQLGAVDTTI
                     CMGASVTVASGISHSGEEREVVATIGDSTFLHTGIPGLLNAVYNGAKMILVILDNRIT
                     AMTGHQPNPSSGTNAFGVPVPPVSLEAICRACGVSYVETVDPYDLVSLLSVMKDARER
                     PGVKVIIARQPCVITARRAGVRRGRFQVDAESCTSCGLCIKFGCPAIEKSGEKAHVND
                     LCSGCGVCVQICPAGAIRKEVKR"
     gene            21501..21574
                     /locus_tag="PKM87_00115"
     tRNA            21501..21574
                     /locus_tag="PKM87_00115"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21535..21537,aa:Ile,seq:gat)
     gene            21616..22074
                     /locus_tag="PKM87_00120"
     CDS             21616..22074
                     /locus_tag="PKM87_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28500.1"
                     /translation="MEKNHVILVVLGLIACVALYFLVDIYAGMIGVILVIALAMSLFI
                     MADSTMLPDVSVRLKEDARGIIVKNRGNAKALSIRLSLIPHDLHYTIASLDEDAENVF
                     PTEMMIDKVKVIAEYQNEKGDLYKRTFTISALDKPDDDLLKPMFPLFKWK"
     gene            complement(22089..22616)
                     /locus_tag="PKM87_00125"
     CDS             complement(22089..22616)
                     /locus_tag="PKM87_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28501.1"
                     /translation="MDQIEKGYRALLKKIDELDAKKEELSEEVRTREAELMGRMGEMT
                     APLVSRIGMNMLKQGKQDTKGEMYDTRYHDQKMIILGKTDPVEHRPDNISKKVDDQFC
                     VLSEDGKFYELMFSTDGIIVDSYRNPLSPADALQIYGHEIMVMLYRAMRDYMEGQKEL
                     LDALEKTIAFVLAEK"
     gene            complement(22799..24055)
                     /locus_tag="PKM87_00130"
     CDS             complement(22799..24055)
                     /locus_tag="PKM87_00130"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNQ28502.1"
                     /translation="MEGSRDGHPPEQGVLRTIAQKKVYIKTFGCTYNEGDSRKIAAVL
                     ERQGCTLVSSPEEAGAVIVNTCTVIGATERKVQRELRSLRNHSLYVTGCMAVVQRDAI
                     LSACDPVIFHPDEIHASYAPPGCMPSGRVGIVQICRGCRGSCSYCITRRARGPLVSYG
                     TDEILREVQSLAGQGAVEIRLCGQDVSAWGMDSGSNLGMLLAEIGEVPGNFRVRVGMM
                     NPATLFPVMDQVAGAFGRERIFSFFHLPVQSGSDAVLERMGRGYTAARALAIVERFRT
                     HCPGITLHTDVICGYPGETEEEFRETLAFLSRMQPDKVNVTRYSPRPKTPAARHKDMP
                     DRFKKERSRILRAHAEKIARSRNEAWIGKEVLVLITEILRPGSSMGRTRQYQGVVVRG
                     NHPPGSTMVVRLTGERTYYFIGEPAR"
     gene            24242..25000
                     /gene="nadX"
                     /locus_tag="PKM87_00135"
     CDS             24242..25000
                     /gene="nadX"
                     /locus_tag="PKM87_00135"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HNQ28503.1"
                     /translation="MIRIGVLGCGNIGHIIAKYRENFEIVALYDQVYPRALELAALSG
                     GNAYQDFESFIASDFEYVVEAASVRAVRQYAEMVLSGEKHLILLSVGALADPNFREDL
                     VRIAEAAGRRIYIPSGAVAGLDNLKVGQISPIHRLLLRTTKSPESLGIRTSERCLVFQ
                     GKSHECIKEFPKNVNVSVALSLAAGRDADVELYADPSVERNIHEIEAEGEFGEIYIRV
                     SNVPSPDNPATSYLAALSILTLLRNLEQPLVVGT"
     gene            24997..25902
                     /gene="nadA"
                     /locus_tag="PKM87_00140"
     CDS             24997..25902
                     /gene="nadA"
                     /locus_tag="PKM87_00140"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNQ28504.1"
                     /translation="MSVKGRIISLKNQKNAIILAHNYQRPEIQDLADVVGDSLELAQK
                     ARETDSPLIVFCGVLFMAETAKIINPGKKVIIPEKDATCPLADQLSPSLILEAKERHP
                     GAPVVLYINSTAECKAHADVICTSANAVKVVRSLPEREVIVGPDSNLAAYIQGQIPEK
                     EIIAIPPGGHCYVHECFTYEDLESARKRGGEIICHPECRPEIQRGSDMVASTGGMVRL
                     VGSRKGNAAWNILTEREMAYRLKTLYPGHTFHVKEDAICEDMKKTTLENLLLALEYEQ
                     YEVTLPPDIMDRARGAIERMLVIGR"
     gene            25899..26747
                     /gene="nadC"
                     /locus_tag="PKM87_00145"
     CDS             25899..26747
                     /gene="nadC"
                     /locus_tag="PKM87_00145"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNQ28505.1"
                     /translation="MTKGPEIPLGLLLSFVREDVPAGDVTSDSLLSGQRCTARIVARE
                     EGVIAGLEEASALFQAHGVMVSPRLGDGEPVDAGAVIATLEGPARAVLLVERTALNIL
                     GRMSGIATMTRRLQVILDRVGTGCRIASTRKTAPGLRILDKKAVILGGGDPHRMTLSD
                     GILIKDNHLELVPLEDAVLKAKAYSRYRKVEIEVQTPDEALMAARAGADILLLDNMSP
                     AVIRECLSRLESAGLREGLLVEVSGGVTEENLPGYAMEGVDLISMGALTHSVKNLDVS
                     LEIIHQ"
     gene            complement(26904..28139)
                     /locus_tag="PKM87_00150"
     CDS             complement(26904..28139)
                     /locus_tag="PKM87_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HNQ28506.1"
                     /translation="MVKKGDMFYAWTTDANLAKKAECGGAVTQLLKFALESKAVDAVL
                     AVKKGQDLYDAVPVIITDPKELDQTAGSLHCGTLLVSKLVKKYLDGANKMKIAMTVKG
                     CDAMALYELAKRQQVNLDNILMIGVNCGGSVSPVVARKMIKEKFGVDPDKVHKEEIDK
                     GQFIIEYEGGHKGISIDELEEEGYGRRSNCRRCKMKVPRQADLACGNWGVIGDKAGKA
                     TFVEVCSEKGAGLLKAAVGAGVIKTEAPNPKGLEIRGKVENAMFKLADKWRKKDFEGL
                     GNGRDRLKKIKDATSRCVKCYACIEQCPICYCVECSTKKPYFVPPGELPVSFMFHLIR
                     FSHIADSCVNCGQCEENCPMEIPNALFMHANQVELEKMFGHVPGQDMSLPLLALVEER
                     EERDRLLATGSDQIFDIFK"
     gene            complement(28143..30215)
                     /gene="fdhF"
                     /locus_tag="PKM87_00155"
     CDS             complement(28143..30215)
                     /gene="fdhF"
                     /locus_tag="PKM87_00155"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNQ28507.1"
                     /translation="MSDITSSKMKYVQTTCPYCGVGCTLNLVVSEGKLVGVEPYRRSP
                     INEGKLCPKGMTCWEFVQSPDRLTKPLIKKDGKFVEASWDEALDLVAKKLKETYDKHG
                     PKSLGFQVSCRTPNEECYIMQKLARVGFLTNNVDNCARICHGPSVAGLSLSFGSGAAT
                     NPFEDVLNSDLILMWGSNAVEAHPLAGRRVAQAKQKGTPIIVIDPRYSPTARLADDWV
                     RFNPSTHIALANSMMYWIIKEGLEDKEFIQKRTKGFEDLKKMVEKYADVENIHGVPLE
                     KVKDIARKYAKAKNAVIIYCLGITELTTGTDNVRSMGNLSMLTGNVGREGVGVNPLRG
                     QNNVQGACDMGAYPNVYSGYQKCELPEARKKMEEAWGLKPGSLPDWYGSTLTEQINQC
                     GDPIKAMYIFALNPVVSYPDSNHVMRSLDKLDFLVVQDIFWTETCEHADVVLPGCSFA
                     EKDGTFTSGERRINRIRKAVDTPGDSKEDWEIFVLLAKKLGLKGFDFKSPKDIWDDMR
                     AVTPSMSGCSYERLEKPESLHWPCPTLDHPGTPILHREKFAAADGLGTFFGLEYRPPA
                     EVADAEYPFTLMTGRLIFHYHTRTQTDRAKVLHYEVPENYVQINTEDAKRLKIANGEK
                     IKVRSRRGESLPIARVTDEVAPGVLFMAMHFPQGANALTNTALDPLSKMPELKHCAVA
                     VEKTKEGN"
     gene            complement(30514..31665)
                     /locus_tag="PKM87_00160"
     CDS             complement(30514..31665)
                     /locus_tag="PKM87_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HNQ28508.1"
                     /translation="MAKKGDMLYAWTNDSALAKKAECGGAVTELLKYALESKTVDAVL
                     AVTRGVDIYEAIPVIVTNPKELDNCAGSLHCGTLLLSKLVMEYAPKLEGKKIGMVVKG
                     CDMMGMLELAKRKLVNLDNIFMIGVNCGGSVSPVAARKMIKEKFGVDPDKVTKEEIDK
                     GQFIIEYEGGHKGIKMDDLEDEGYGRRSNCRRCKVKITRQADLACGNWGVIGDKAGKA
                     TFVEVCSEKGAALLTKAVDAKKLSTEAADPKGIEIRAKTENAMLKLGDKWRKRDFEAL
                     APKLWESIAEETGRCMKCYACIDHCPVCITRPDKFDQPDLMVRAGFVPADPMFHLRRF
                     AHISDSCINCGQCEECCPAEIPLALFSHAIRTEADKFFEPKLGKSAYTN"
     gene            complement(31666..33738)
                     /gene="fdhF"
                     /locus_tag="PKM87_00165"
     CDS             complement(31666..33738)
                     /gene="fdhF"
                     /locus_tag="PKM87_00165"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNQ28509.1"
                     /translation="MAKASEPVINYVPTTCPYCGVGCSLYLVVNDGKVVGVEPNKRSP
                     INEGKLCPKGMTCWEHVHNPDRLAKPKIKKNGKFVDATWDEAIDLVAKKFKEISDKHG
                     PKALGFQSSCRTVNEDCYALQKFARVGFKTNNVDNCARICHGPSVAGLSLSFGSGAAT
                     NPFEDVLNADLIVMWGSNAFEAHPLAGRRVIQAKKKGIPVIVLDPRYSATARLGDEWI
                     RFNPSTHIALANSIMYWIIKEGLENKAFIAERTKGFDDLKKTVEKYADAEEIHGVPLE
                     KVKDFARKYARAKNAVIIYCLGITELTTGTDNVRSMGNLALVTGNVGRPGVGVNPLRG
                     QNNVQGACDMGAYPNVYSGYQAVAVADNRAKMEKAWGMPAGSLPDWYGSTLTEQINEA
                     GNPVKAMYFMGLNPVVSYPDSNHVMRQLEKLDFCVMQDIFWNESCEYADVVLPGTCFA
                     EKDGTFTSGERRINRVRKAVDGPGESKYDWEIIGMVAKKMGLKGFDWKTAEDVWDDMR
                     ACTPNMFGCTYERLGKPESIHWPCPAVDHPGTPILHIGKFSAADGKGTMFGLEYRQPA
                     EVADAEYPFTLMTGRLIFHYHTRTQTDRAKQLHYEVPESYIQINTIDAKKLGIKENEK
                     IKVSSRRGQIETLARISDDVAPGVTYMSMHFHKGANTLTNTALDPLSKMPELKHCAIK
                     IEKIAGAK"
     gene            34243..34881
                     /locus_tag="PKM87_00170"
     CDS             34243..34881
                     /locus_tag="PKM87_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HNQ28510.1"
                     /translation="MPTIPEYTPGELLAKMESMGIPESLRESFRKCIDFHTYAAPGML
                     IGVFMVDCALKILGRRPEEKIFVTCETHKCLPDPPQVIMHATTGNHQLRVLPIGKFAM
                     TFTPSSVKEYAEGIRIYVDRKKLDQYPALASWYDNSAAFKPATMKRQLAEEILAAACG
                     MFSHERVRMKVTHKKKWNSVTCPSCGEQVPQDLMEGTTCAGCGSLAYYEKVT"
     gene            34947..35780
                     /gene="tsaA"
                     /locus_tag="PKM87_00175"
     CDS             34947..35780
                     /gene="tsaA"
                     /locus_tag="PKM87_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HNQ28511.1"
                     /translation="MDNSLVLTPVGVVHSPVHESRIMPVQGVRGQIEIFPEFVPALEG
                     VETSSHLILLAWMHRGERDLLTARARKISEDLPLKGVFSLRSPSRPNPLSVSVVRLLC
                     RKEGGILEVDSLDCIEGTPVIDIKPYQPGWDCVFSATHHDRSVKIEKMGPSEYRQMLI
                     REAVNYHGEWCPGAAIAVRVAECATRALGGDLTRPEVHLFCGGHPCITDSLIGITAAR
                     RGNGRLHPVAREENRGLECCEFSIKRYRVVFSISRLPEDIPYILSCRNEDLFSTRVFT
                     T"
     gene            complement(35792..36589)
                     /gene="fdhD"
                     /locus_tag="PKM87_00180"
     CDS             complement(35792..36589)
                     /gene="fdhD"
                     /locus_tag="PKM87_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNQ28512.1"
                     /translation="MKIFENECDSGPMISEYDVISVDADGHGPRRVQVCREERVHLEV
                     NGSHIASLTVSPRELAEFACGYCVTEGIVPGIFAISEVEVEGDRIHVAAPAIEREILA
                     RPKEIRTSGGVGVIQDEHTMRVPLPIAARVSRSVIYCAMECLNDHAVLWRETGGTHCT
                     VLYDTTGTAVSHAEDVGRHNSVDKAIGKALLSGIDPGKTFLCCSGRMPEGMVSKVYHA
                     KIPIVVTNNAPSAAGIDLARRVKLTLAGFVRPPRMNIYSVPERILLG"
     gene            36770..37606
                     /gene="modA"
                     /locus_tag="PKM87_00185"
     CDS             36770..37606
                     /gene="modA"
                     /locus_tag="PKM87_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNQ28513.1"
                     /translation="MKKNLFRVTPVTLGTLVCILMFLGAVTPGCITSKETTPTPGVSQ
                     AGILVYSGAGLKSAMTDIGQEFTRKYGIEVQYNYAGSGVHISQMNLTRKGDVFITGST
                     VEYNNAKNQGLVGPYQLVAYHVPVIAVQKGNEKNITMLQDFARPGLKIALGDVNATAI
                     GKAGAKMFKKLNITDAVEENVVTRTPTINELTILMTAGQADASILTLDLINDKTMEAI
                     QIPITVNEVMIIPVGITTFSENQDNARKFADFVASDEGKAIFVSHGFPKYPDPAYADV
                     KP"
     gene            37637..38134
                     /gene="tsaA"
                     /locus_tag="PKM87_00190"
     CDS             37637..38134
                     /gene="tsaA"
                     /locus_tag="PKM87_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HNQ28514.1"
                     /translation="MLIEYAQIGVAHSPFRNAGAPPVHSTFTQAKGTIEIFPEYREGL
                     GSLEGFSHLIVIYHFDRAERRALKEPPLVDGETPHGIFATRHINRPNPIGISYVQLEK
                     ITDGILIVKGIDLLDGTPILDIKPYIPAFDSIPDATNGWVTPQHIEKIRNVSLLATRD
                     NDTMQ"
     gene            38162..38935
                     /locus_tag="PKM87_00195"
     CDS             38162..38935
                     /locus_tag="PKM87_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ28515.1"
                     /translation="MKRFSLILTYLVLVAISAVLLGLVTYSPLPDLVASFASPEIQFA
                     IVLSLVTSFASTAICIVIAIPVAYALARFDFFGKRIANLALTLPLTLPPLVAGIALLL
                     FFGTTPWGKALEQAGFMVIFTPLGIIVAEVFVNIPYMIRILRSAFSTINPRYEYVAKT
                     LGCTDTGAFFRVTLPMARSGLLAGTVITWSKAMGEFGAVLMVAGATTMRTETLPIALY
                     LNISTGDLDLAVAAATILILISLVTLCAVEYFDKGVNVF"
     gene            38949..40010
                     /locus_tag="PKM87_00200"
     CDS             38949..40010
                     /locus_tag="PKM87_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ28516.1"
                     /translation="MLRIDSVSITLGEFSVRDVSLEIRPNEYFIILGPTGAGKTVLLE
                     TIAGIHTPGSGKIFLGNQEITSIEPRLRNIGMVYQDFMLFPHLTVRENISFGLKQRKL
                     PVDEQSTMVDEICALLEIPHLAGRYPGSLSGGEQQRVALARALVLKPEVLLLDEPMSA
                     LDRRTRERMRTELSRIRKLTGTTIVQITHHFDDVFAIADRIAIMREGRIVQVGETADV
                     FLHPADTFVAEFLGIGNIIRGTSSRAGYITVITPDTGPVFYASSRVSGDVVATLHAED
                     VIVSVAPFASSARNCLYGTVSEILAFGSTIRVIIDVGFPLTALLTRESCHELQIEPGS
                     RVYATFKATAVHVIPVNPE"
     gene            complement(40125..40877)
                     /locus_tag="PKM87_00205"
     CDS             complement(40125..40877)
                     /locus_tag="PKM87_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HNQ28517.1"
                     /translation="MSDFHILDDLLATLPEDPVPVRSVLVGVHWTAVCSRHCGLATTI
                     IANKPHDHATFVRNVGNLHQKTAQELAEYSRSKNRLEASIGVAAINSLLFMNQKDAVE
                     MNASEVLIKKGAGKNVALVGHFPFIPQVRRRVGNLWVIEQQPAEGDYPANTAADLIPQ
                     ADIVALTGSALINHTLEDLLSLCRPDALVMVLGPSTPLSEVMFDYGVSIIAGSQIIDE
                     AAVMRTVGQGATFQQVEGVRKLMYARRGASLV"
     gene            41217..42119
                     /locus_tag="PKM87_00210"
     CDS             41217..42119
                     /locus_tag="PKM87_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ28518.1"
                     /translation="MPSKSEMIRINQRIARSGGVICERDPRFSTVRLRFAGGIATREH
                     LLGLARVVRRLGLGPLHLTTRQTVEIPHVENEKVPSLLLSLEKIGIALGAEHDEVVNI
                     TTCPGTERCRLANIATGTLREDLDRVHFGKQMPIRVRIAVSACPNGCTSERLSEIGIT
                     GLRTPIRNEGKCTGCGTCANTCKENAIVMVNGRLTLDHEKCMECGMCIDSCPFSIIQG
                     TEPQYLITVGGRRGRHPQLGRELIRVSSEKNAVAVVNRVVDWIYRNAYSGKTLTDQMD
                     AMGFTRLQEKTWKEIGESSSPTVK"
     gene            42306..42569
                     /locus_tag="PKM87_00215"
     CDS             42306..42569
                     /locus_tag="PKM87_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNQ28519.1"
                     /translation="MAFSVHVNMERCTGCGNCVAACPVNALEIYTIDPVTSEKIYSVR
                     DGKSISLDLKLELCAGCGVCVEACPYKVITLSGRGAYTPPVLT"
     gene            42626..43039
                     /locus_tag="PKM87_00220"
     CDS             42626..43039
                     /locus_tag="PKM87_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNQ28520.1"
                     /translation="MAEKIILNMITQRSIEEGQAMEKGKTTPEYFAACSVCEMNPEDM
                     KKLGIWRNTNVRVTSEVGSVIVKAIEPTQYCPPGLAHIRQGVWANQVVPPRTQSTGTP
                     QYSGFPVTIEPAINERIKTALELVQGSVGLWKGGN"
     gene            43040..44413
                     /locus_tag="PKM87_00225"
     CDS             43040..44413
                     /locus_tag="PKM87_00225"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HNQ28521.1"
                     /translation="MPKWITQVGCPYCGSSCDDIEVLVSDDGKKILETRNACVIGNEI
                     FHHVSSPNRPKRPRMRQPDGSIKEVSYDEAIDYTAKTLLKAKKPLIYGFGSTNCEGMS
                     AVARVAEKAGAVLDNCASICHGPSFLAIFDNGYPSCTLGEVKNRADVVVFWGCNPMHA
                     HPRHTSRYSIFPRGFFTNKGQMQRTVVSIDPRETDTAKLADYHFMLNQGHDYDLFDAF
                     RTVLRGHDIPPEVAGCKREMIKEVAEKMKNARFVMIFFGMGCTHTDGRNHNIDIAISL
                     TRDLNTHTKASIMAMRGHYNIAGPGQVWSWQFGFPYCIDLSKGTHAHMNPGETSSIDL
                     AMRDEIDCFVNVGTDAGAHFPIAAVKHLKKHPFITVDPNICMASEIADLHIPVRVVGV
                     EEPGVVYRMDNVPIQFKKVVDGPEGVPSDEELFDKVYARMCELTGCEPIWVHAKEHAK
                     AATSTEH"
     gene            44415..46121
                     /locus_tag="PKM87_00230"
     CDS             44415..46121
                     /locus_tag="PKM87_00230"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HNQ28522.1"
                     /translation="MAEILIKNGFVIDPSRKINGDVADVAIKDDKIVDKVGSGARVID
                     AKGKVVMAGAVDVHAHVAGPKVNVGRLFRPEDKLFRSPNAQENYRMEMGFSVPSTFKT
                     GYDYARMGYAFVMEAAMPPLHSPHVHEEIHDTPIIDEAALPVFANNWFVMEYLKNNEI
                     ENSAAYVAWLIAATKGYGIKLVNPGGTAAWAWGLNCLGINDPVPFYDITPAEIITGLM
                     KTNEYLGLPHSIHVHQNSLGEPGNYTVTLDSLKLAEGVKARNKFGRDSVMHSTHIQFH
                     SYGGDSWANFESKAKEVMDYVNAQKNITVDLGCVTLDETTTMTADGPFEHHLNNLNHL
                     KWANVDVELETAAGVVPYVYDKNIKVCGIQWAIGLELGLFAKDPMRTFVTTDHPNAGP
                     FTRYPRIIKWLMSKKAREATLDSFKYKDKVIDATNIHSMDRELTLYEIAQMTRAGPAK
                     SLGLSHMYGGLGAGLDANVAVYDLNLKNMPSDPEMIERALLRASWFIKSGQIVVKDGE
                     IAGHGNKRTVWVNAKVKENPQVTRDIHEKFLKNYTVGIQNYPVWDSLAPNPLVIDVDV
                     EA"
     gene            46125..46928
                     /locus_tag="PKM87_00235"
     CDS             46125..46928
                     /locus_tag="PKM87_00235"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HNQ28523.1"
                     /translation="MAKITLKPFKTPELFLECPIISPDMFAGKTPAEIAELEVREGKT
                     TQKLGDYFGVTGSAGKDAGETEILIDGDCSRVKYIGSKMTAGSITINGNADMYIGGWM
                     KGGKIHVKGNVDSFCGIAMEGGDIIVDGNAQNYVGCAYRGDWRGMRGGTIHIHGNAGN
                     DIGTFMLGGTIIIEKNAFIHVSTHAEGGTVVIKGDVQGRVGGQMVKGDIYVLGNIKYM
                     LPGFKEIDRVKKEIAGETATFKHYIGDLGERHPKSKGKEIYANLFVRAS"
     gene            47007..47753
                     /locus_tag="PKM87_00240"
     CDS             47007..47753
                     /locus_tag="PKM87_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNQ28524.1"
                     /translation="MTDPGIEFLEKTRYPDFSTVDLIMRVPEPPNELPVTENARVISL
                     PSPKKVKITDVPVREALENWKPPGFFSRSSITKNDLSFLLWCMQGVRKADGDSPMLRN
                     VPSSGSRYPIETFFVAGEVEGLETGLYRYLPLSHRIVVERLDSDIPFAMGTASMNFNL
                     ITRAAVTFLFVGIPYRSTWALGNRGYRSVLIEAGHICQALIMASSCIGCEVQPIDLFH
                     DQMMIQLARLDPKTQWPLLVGTVGRREREL"
     gene            47885..49123
                     /locus_tag="PKM87_00245"
     CDS             47885..49123
                     /locus_tag="PKM87_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNQ28525.1"
                     /translation="MDGNIFFTSEPLTAERLSWLVELLKYYTTRLFPESLHHNPRTPT
                     PPFTFFLLGDACNILIGREHQRSLEIFFRLPCFRCIFDQGDLHVRRISIEPFRIRYPG
                     QVIPIAPGDNLPGRSIWDCLMNAMGKTPGPPSIGFLQMRSPYMFQSSSCVVDLFRAAA
                     RTGISPEFYGYLDGVHAMHRDQRPPHHVNIGEALSDIYRVAFTKGLFPRYLICQESAA
                     SRGYCTFSGDNGRVVSASLIPQARIKSLDHIVSRFCMSHRIFSHTSFFVDVVVQRKIP
                     SVKFSAERKKPSLVILASHSPYGTEFTKGAISLAVACAHQNIPTRIVFIEDGVYTLTG
                     SESPAGMWADMDMHALIEATSRMDTLEYYVYTPSSQARGIAINPSIKGVCPVNPTEFS
                     QVLLTPPAGLEVDHQRVLVF"
     gene            complement(49249..50670)
                     /locus_tag="PKM87_00250"
     CDS             complement(49249..50670)
                     /locus_tag="PKM87_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ28526.1"
                     /translation="MNREFQGKWVDTPEPCYRLDLRLGIPRSRGGEELEWLIGDQGLC
                     RHPDQHPSLILADILSEPHDDPTPIKSAVKKCSLDSGPIKVKLSSTTEKAGDLAALSY
                     TVRPNRGLCEFIRALSDTFVLGAPEGSLVSCMGKTTPWVPQSTVTAHEPWQALVRVKR
                     RPEGYMRNRMDKSTLLQPSKMRHDFPCGERHSRDREGCRVFSDTTQRKPLVFDILRII
                     LRKGRTPVAEYDLYLRSWLAYPESTRGIYERRALREFRIQKGYQMTRPDWSETPRIYV
                     ISDLHLGHENAVPRYKRPFPMGKISEMDRVLIRNWNWTVRKNDVVLFLGDLSFRSQES
                     SESYLQRLEGRIFYLEGNHDLYYSYMSHCLFMNYRGARYLFIHDPEELVRPFDGWVIH
                     GHVHNKDLTDFPFFNPFEKRVNVSAEMVGYRPIPLEEIHRLVQESNDVLDFYPGRAKG
                     AEEGEKGGIAAPGTGGMLPLVHD"
     gene            51000..52847
                     /locus_tag="PKM87_00255"
     CDS             51000..52847
                     /locus_tag="PKM87_00255"
                     /inference="COORDINATES: protein motif:HMM:NF011068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HNQ28527.1"
                     /translation="MKRRYLSLIPFEDALEKMRKTAPSPRKVETVLLDSAVGRVLSEA
                     VYAKFSVPGINVASMDGLAVHSSDTRNAADRSPVVLSDAVRINTGQPLPPSYDAVIMI
                     EDVWEEDGKWLIRKSAAPWQFVRPAGEDIRKGDLILPRRHLIRAIDIGGLASYGIAFV
                     NVLSVKVGIISTGGELVPLGMTPSPEQGVESNNFFAEAYLSRMGATCKRYPLVPDDRE
                     KIAAQLLLAVRENDLVLLSAGSSAGSTDYSAEILKDSGKLIFHGVGIKPGKPVMMGIV
                     EKTPVLGMPGYPVAAHTVVREFAARLLEYWGMAPHPTYTVKARLGQNLSSDLGYDEFV
                     PVSTGKVDGRYVVIPLSRGFGVQSALLKSNGYIHIPSSAEGLEAKQEIDVTLYENPSF
                     MERSLLMVGSSDTPLQILADCMVDRSITLRCCPTSNLGGILALRAGICHAAPVHIPIL
                     KDHTIPDQLKAQKGLSIRRLVIAEQPFGLATRNAVRLEDLKNLQIMNSQRGTANRLLL
                     DAVLARDGIPVSALKGYENELKNPDMIASNISSMIADAGICTQRSAANAGLRFLPLGI
                     DAYELVMRADTIEDPRVKEMIEMIQSDEFKERLVQNGGFDTGSTGKFRI"
     gene            53398..53925
                     /locus_tag="PKM87_00260"
     CDS             53398..53925
                     /locus_tag="PKM87_00260"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNQ28528.1"
                     /translation="MTEKKPPEQRIHAEEEARRRRVRGLIKLLQSKYLDDRWKAAEAL
                     ERVGDATAVQPLIDALDDPFVDVSWIAAKTLGQIGDGRAFEPLLRALGSKEKWLRIGG
                     AIGLGRLGDRRAVPLLLESLKDPQALVRKHAAWALGMLGDESSRKALEEAQVDPDKRV
                     REAATEALARLDASS"
     gene            54016..54516
                     /locus_tag="PKM87_00265"
     CDS             54016..54516
                     /locus_tag="PKM87_00265"
                     /inference="COORDINATES: protein motif:HMM:NF021612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HNQ28529.1"
                     /translation="MIEWIILLLIAVATLIAWRYFALRTTLEQRAAALFEAWRAERME
                     TEVKARADLLHREWTLQEEDRVRRDAIGRSGAVIRGKVTEHLTPYFPEFPYDPRDARF
                     LGTPVDLLVFEGLAAGSLERITFIEVKTGKTGALSNREKQVRDCVQKGLVRYEVLHLR
                     GDEKDS"
     gene            complement(54521..55120)
                     /locus_tag="PKM87_00270"
     CDS             complement(54521..55120)
                     /locus_tag="PKM87_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28530.1"
                     /translation="MNGEATMKIDFSRLGEDRLFLVLSSAERLKVNNIAIIRTLTGQG
                     YRVVVITSNHPSAVLKRLYEAEGIEMARVSFIDTITKYAMGKVPPGQEDCRFINSPSN
                     LTDLGIAITEVLNTLSGEKACLLFDSISTLLIYIPSTNISKFIHFITSKLRLVDASGI
                     FLAVEKGLDPLLLTQLTTYVDGTIDMDAQPPGNEVQAEK"
     gene            complement(55117..55782)
                     /locus_tag="PKM87_00275"
     CDS             complement(55117..55782)
                     /locus_tag="PKM87_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28531.1"
                     /translation="MNPEGRGEGAGSRVKCDDGPANTTHRLWILVLLGMICIALEIGV
                     HLFLRTAIGYTHIFYVLLVLAAVWYYQKALYVAGALVAATLATSLFVGDFTWATLLRA
                     AMFLIVTWIVAQISEERDRAKAEILVQKSAIEEKHYVLVGYLAEVALRMKNPIQILHD
                     NMAALLLQLQGDKPDIDSVKMDLAVQISHAEQIMANFRDLNQAIVEEREEIPVAYRDF
                     LTR"
     gene            complement(55845..56222)
                     /locus_tag="PKM87_00280"
     CDS             complement(55845..56222)
                     /locus_tag="PKM87_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28532.1"
                     /translation="MILVRQEKVAVLILVLVVLAVLVSAFVLESVGKGAFSLPYAPDS
                     AEGSLVHHQGMVEESTITASGGHQVLVVSGVKVFIPSEHVQNGWPRPGQEVSLYGTVQ
                     TYRGEREILVASGEDIASLQSPV"
     gene            56295..56522
                     /locus_tag="PKM87_00285"
     CDS             56295..56522
                     /locus_tag="PKM87_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ28533.1"
                     /translation="MPEMNLPPSSLTVLHILDEGGAMTHKDLVKKTQLAPRTVRYALK
                     KLKEQHLIIEKFNFKDARQIIYQNRPVAVTA"
     gene            56519..56926
                     /locus_tag="PKM87_00290"
     CDS             56519..56926
                     /locus_tag="PKM87_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ28534.1"
                     /translation="MKGRACDIMVCDAMVRRYLPAMRAEMVFRLVHERGLSQSDAAKR
                     LGITRAAVSQYLSRKRGDATFELSPDMNALIDRWVMAVSGRDDGITLCDICRCAMKDP
                     VQQETGVFIRGREGDAGAFPPSSSSTRREEGQR"
     gene            complement(56847..58541)
                     /locus_tag="PKM87_00295"
     CDS             complement(56847..58541)
                     /locus_tag="PKM87_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNQ28535.1"
                     /translation="MIRTTIMLLLVLAAPVTGIELVEFCPDPYLPDDRDEYLCLEGWG
                     QADGILVTDGEGGIRFPPGTTLRGLTVVAMDAEAYRNSHGEYPDFEWDGSSPVVPDMI
                     RTGRFQLSNTADEIEVFSGRVCLQKVDWPGTVSPRQGQVHSFSEGMWDERVLMLGQSR
                     FEPAMFPNASGVAFVSPDCSREVFLAMVGGAKEEILVSIYEFTSPALAGSLEEAHARG
                     VNVKVLIEGGPVGGIPEEEHAVMAQLLGCGIPVRAMQGTALVHSPYRFLHSKYMVIDS
                     NHVMVTSENFKENGFPERGRRGNRGWGLAVTHPGLAEYFRSVYTYDAGGEWAVYAVPR
                     QGDPEIASSRTYTAEFSPYVFEGATVVPVISPDTSGLLHSLIQGARRSVDIEMAYITN
                     ETTLCLNPFLADAVDVSRQGIPVRIILDSYYYNIDGKADNDEMVGLLNAMAASEHLPL
                     EARCAYLGPDSVEKIHNKGVIIDREKVLVSSINWNMNSPTFNREAGVIIHHPGVGEYY
                     SLAFERDWEKAGSEKGEKGPDLIKIAVACVVIAALLLFWYWRRRGGRHRHRPRDHV"
     gene            58602..59195
                     /locus_tag="PKM87_00300"
     CDS             58602..59195
                     /locus_tag="PKM87_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridylate kinase"
                     /protein_id="HNQ28536.1"
                     /translation="MTPVRPPLVLKIGGSLQDCAGSICALLVDLDVPVLVIPGGGAFA
                     DLVRECRLEGTAAHWMAVSAMEQYGWYLSSFGLPVTTRLDIPGGPAILLPYGLIRAKD
                     PLPHRWEVTSDTIAAWCAHTLHLPLLLLKSVDGIRVEGRLCPRIRSPVETDDVDSCFL
                     PFVLRRRMHALVISGRHPDRLRAALTGLPTIGTTIGF"
     gene            59242..59403
                     /locus_tag="PKM87_00305"
     CDS             59242..59403
                     /locus_tag="PKM87_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HNQ28537.1"
                     /translation="MPIRKCTSCNSPLAEEGAAEFGCPKCDYQIRRCYRCREQSIPYT
                     CPKCGFGGP"
     gene            59407..59664
                     /locus_tag="PKM87_00310"
     CDS             59407..59664
                     /locus_tag="PKM87_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 1-beta"
                     /protein_id="HNQ28538.1"
                     /translation="MGTVAIILKVMPESPEVDLAVLKREIQEKVPSVRDIREEPIGFG
                     LKALKVAAIVNDAGGESDAVEAEIQKISGVERAEIVELTLT"
     gene            complement(59716..60663)
                     /locus_tag="PKM87_00315"
     CDS             complement(59716..60663)
                     /locus_tag="PKM87_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNQ28539.1"
                     /translation="MSKVTIVGATGNVGTFSAHTLAGIPYVHEILLHGRAGREDLLCG
                     ITQDLIDSFAASGQAVDLSWTTDIMDARDSDIVVCTAGVPRRPGQDRLDLALQNARIV
                     ADTARVIGKCAPHAMILMVTNPVDVMTSVALRYSGMQPNQVFGLGTHLDSMRLKSLIA
                     KHFHVHVSEVHTRIIGEHGDSMVPLWSATTIGGIQISNLPAFSRLPVEEMVATVKSSG
                     EQIIRNKGATVYGPGEAIATLVRTVLGNEDRILTVSAYIRSEVHDIGDVCIGVPARIN
                     RSGVFPVPIKIAEDEVAQFGVSVDKIRAVTHEILQKLRE"
BASE COUNT        12943 a        17434 c        17119 g        13210 t
ORIGIN      
        1 ttgccagtgg tgtaatggcc gagcttcttc tggacgagag agccgaccgc gaggatatgc
       61 agacattcat ggccacgagc gggaccaagc tccagcccga ggacatcgac cgcgtcgtga
      121 acaacatggt cgcctggaaa cccgactttg ccatcgtcgt ctctccaaat ggcgtgctcc
      181 ccggccccac cggtgcccgc gagcgcctcc tggcctccgg tattcccacc gtcttcatca
      241 ccgacgatgt gaccaccaag aaggagtggg ccgagatcaa ggacggcaag ttcggataca
      301 tcatcatgaa gggcgatgca atgatcggtg cccgcaggga gttccttgat cccatcgaga
      361 tggcagacta caacggcaac ctcgtgaagg tgctctccgt cacgggagcg ttccgcaagc
      421 tccagatggc actcgaccag gtgatcgacc aggtcaaggc cggcaagaag ggtgcagaga
      481 tcgcgctgcc gaagctcgtg gtcacctccg acaaggctgt cgacggggag ttcaccaacc
      541 cctacgccct tgcaaaggcc cgcgccgcat acgagatcgc ctctgcggtc gcaatggtga
      601 acgtgaaggg ctgcttcatg acaaaggagt gggagaagta tatccccatc gtgtgcagcg
      661 cccacgaaat gatgagagtc gccgccgtgc tctgcgacga ggcccgtgaa cttgagaagg
      721 catgcgacgg ggtcatccgc aagccgcaca agaaggacgg cgtcattgtc gcgaagacca
      781 agctgatcag caagcctgag taaactcttc tctccctttt ttttagtcgt cccggaacgg
      841 atggtataca tttacacgcg atgagtatcg agggtttaag ccgggcggac agatcccggg
      901 aagaatgttt cagatccggg attttgaaaa ataagcgctc aattcttccg ggagcgtgag
      961 aatgactggt tcctggtgga gacgctccat gaacgccgag tcgctggtag atgacgcatt
     1021 ggggtttatc cgcgagatga cagagcagaa cgcagagaac ccacgtgatg cgccaggacg
     1081 gtcgaagaag agagacatgt tgaatgcatg ggtcccaagg ctcctgtaca tcgaaagaat
     1141 ggagaggagc cctttcacaa aggtctcgat ataaggttca aaatcgttca tgcaggagat
     1201 ggggaggata gccctgacct cccgctcccc gagcggcacc gcctgtgcaa gccagcatac
     1261 ctcgtcgccg aagaggtatc tcggaccagt ccgctccgca caaagaaagt catcccagta
     1321 actgtgtcca tgctccagta ggtagcggaa ccctccttcg aggtagcgtt cggccagagc
     1381 gggtggcctc ctgtcaacca gcccctggag gtggggatga gcgatcgacg cccctgctga
     1441 ggggaggtag ttccagttga gggaggcgta tccttcatgc tcttcaaggg actctaccat
     1501 gccctcgagt gcgtcactga cctggcgggg cttgaactct tcgacgtggt gggcccgggt
     1561 gatgactgtc acggtatgca tctctgcata gggaaagagg ttcgggaatg tcaggctctc
     1621 gccctttacg atgcgggttc ctcccgggaa gcagggggtc acctcctcga cctgtcctgg
     1681 acagaacgga cagggcggta tgagtgtcgg ggggacatac gcaacgtcca gcctgcggtt
     1741 gagccgttcg ggggagattt tgcacctgat cccggatatc tgctcttgac ggtactcgat
     1801 gacatggtcc ccggatcgga cggtggtgct ggtgaacatt cctctccatg cctctcatgc
     1861 ggggagccga tccccgatca gtcggaaaaa aaagtggtgg tacttgtgct cgactgcgcc
     1921 aattttttag acaatgtact tgccgaggag gcggatcgag ttggtcatgt ccggcccgag
     1981 cggggatccg aagatgatct gggtgacacc ggcctttgcc aggtcctcgc acttctgctt
     2041 gaccgtgtcg ggggttccgg cgatggtgaa ggcatcaatg gtcttgtcat cgacgttctc
     2101 tccggcggtc ttgaagtcga actttgccag ggcattcttg atgtttgcca cctttgcggc
     2161 gtcgagacca tggcgctgga gaaggtctgg gggagagccg gcggcgatga aggctgcgac
     2221 gatcttggcg gcgttccggg ccttcttctc gttcgtgtcg attgacatgg cggtgtaggc
     2281 gccgacgtca aagcccttct tgcctacctt gtcgcatgca gccttgatga tcgggatggc
     2341 cacggcgaag tccttcgggt tggatgcgtt gatcagtgca ccatccccga cggtcccggc
     2401 aagctcgagg acttttggtc cctgcgcgcc gacgtagatc gggataccct tcttgccagg
     2461 gaggttcact ccggtcagct tggcaccgtc atagtcaaag aactgcattc ctgacttctt
     2521 aacctcctct ccggcacaga gcttgcggat ctgggcgatc gcctctccga gccttgcgac
     2581 gggtttctcg gggctgatgg caagcttcgg gagggtggac aggtcaccgg gtccgatgcc
     2641 gagcactgca cggccgtccg agatctcgtt cagcgtgcac gcaaacgagg ccattgcggc
     2701 gggcgtgtca gtgaatgagt tcatgatccc cggccccatc ttcagggtgg tggtggcggc
     2761 agcgatcatg gcaagagtgg ggtagcagtg gcggttgttg tagtggttgg tgatccacgc
     2821 gtagtcaatg tccttcgact ctgcgagctt gcagaaattg accacctgct tgacattcac
     2881 attgccaggc acaaattcta ttccataggt tgtcaaggta acttcacctc atggagtatt
     2941 atcgaacgaa gatttaaata cattatcatt ttttattgca ggaatgtggg gataatcccc
     3001 gattcgccga aaaataccgt caataaaggc cccgattatg atttatggtt tgcatttaat
     3061 ccaagacgca gagagatttc agctctaggg aaacggaata ccctgcgatg ggtgagaccc
     3121 ggcagggggg ttcatggcga atggtctcct cccaggggcc gcgtgcaccg gtcctgtccc
     3181 atgcgaaaat atctttcgcc cggccgaaat gagcagaagt gctttattat tccagggaaa
     3241 aattcacatg aagagagtac ctgggaatat ccatacggga atgggtagga aacgttgtgg
     3301 gaaagcccac attccaggga gtgcgcatga tatgccccgg cacactcacc ccatcccccc
     3361 tcacctcagc agattggact caaacccgaa gataccatgc gaatactcac cattggcctg
     3421 ggtggtgccg gatgccggat agtggaccgc ctccttgctc acgatcaaaa ggccccggtt
     3481 cgctgtgtct ttgggatcgc catcgactgc gatgccgggc agttgaactc actccagcat
     3541 gtgccggtgg atcatcggct cttcttccag ccgcttgacc ctgccctgtc cggagacctc
     3601 gcggcacacc tgccttcaga ggaggcactc tcgcggctcc aggcccttga tgacggcgat
     3661 atcgatgcct tgttcatcta tacaggcctt ggcggcacca tggccgggac agcccccgga
     3721 ctggtctcgt acctgaaaga gcgcctggca gagccggtat tctgcctctg cactctccca
     3781 tgtctgcagg agggtgatga acgctgctct gctgcggcag atcacctgga cacgctgcac
     3841 ggcctgcttg acgggatcgt cctcttcgac aacgagtcct ggcggggccg ggtctttcct
     3901 gctcctgatg aaccgggtat ttcacgtgcc ggtgacatcg gcgcccttgt caggcggcgc
     3961 acggagcccc cgccgccggt ttcggagacc gaactcttct accggaattt caatgacatc
     4021 atcagccggc gtttcaccct tctgctcaga gcaggggagt actccgagcg ggctccggga
     4081 gatctgccag aggtggtact ggatgcgggc gagattttaa acactatcca ggggatgggt
     4141 ttcatcacca tggggtatgc aagagaagag gtgccttccg ggagcccaat ggacctgctc
     4201 tccaggttcc gcccggggac accagcggtc caggacgggc acctgaaagc ttcccgtgtc
     4261 gtggagctgg cgaaaaaagc gatattcgag gagatctctg cgcccaccgc acttgaggaa
     4321 gcgaagaaag cgctcatcct catcgcaggc ccctcgcaag agatgagcat gcgagggttc
     4381 atgacggtga ggagatggat cgaccgcacc atcaggggac tggagctcag gtcgggggat
     4441 tatcccgtga gcgatacccg tttcctcggg atcttcatca tcctggccgg catggagagc
     4501 ctgcccgcag tggatatgtt gaggcagatc agggacagga tgaggggtat gcaggcagaa
     4561 ccggaagaaa gcccgggaac ctgacctgat gagagaatga tgaatcaccc gatcgccaat
     4621 accgcgcttc ggtgcggcgt ggttacctca ttcatgatgt atttttgaaa acgggacgtt
     4681 ccctgcccct atttcagtcc ggatagggac ccctttctcc acggtgttcg ccacgaagtt
     4741 catgccagag aatgcgacga tggcaaggcg gtagggatcc gggaccaggt tgaataccga
     4801 ggtccccatc gcgacaggaa agacaaaacc tgccttcttc atctgcgcca ccacctcacg
     4861 ggcccttccc tcggcgagtg ccgggatctc ccggacgttc gcgaggcccg tgccggtgcc
     4921 ggtggaaacg agcgacgaga gcgatgtcag cccggcagag atgaagagct ggagcgggtc
     4981 gatggtcgtc ccccgatacc cgatgagatc cacgaaacgg gtcgcctcgc catgctggat
     5041 gtccagtcgc ccgccatacg ccatcctcac cgggatgccg cgacgctgga agacgccgtc
     5101 catcgtgatg ctgcagatgc tcatgatccc cgcgctcccg ggtggaatcc tggggtccct
     5161 gtcaataacc cggtaactcg agaaaaaacc gcatttcgca gcgataacct cgttgaatgc
     5221 cttcattgca tcagggagcc tctcttcagg gacgatggag aggttgtagg cgacatcccc
     5281 ggtgcctgtc tcggggtcga acgtgctccg gaacgcgagg cgctcgagtt tggagatgat
     5341 aaatccgatc ctgtccccta ccagggcact ctcggtctcc tctttgccgt agggtgtgag
     5401 gatgcggccc atgttcccga ctttctcggt gaaccccatc tcgtcgaggt accggagata
     5461 atactgcacc gcacggtcgc tcaggacaaa acccctctca gccatcaact ccgagaggcg
     5521 ttttgcaccc atcggctccc tgtgctcctt gagtattctc aggatctcga tgtattttcg
     5581 ctctgtccga atcatttatc tcgccttgat gacccctgtg ctgtcctggt aacgcccgct
     5641 gtaggcattc ttcccatcgg tgacctggtg gaagatgatc tgcactaccc tgtcgttctt
     5701 tcgcaaggtg aggtgctctg cacccatgtt gaccaggcag agtgtcaact ggccccggaa
     5761 accagggtca acgaaacctg cagtgaggat aacccctctc ctgccaaagg atgaccggca
     5821 tctcatcgtg gccgcaaggt ccgaaggcag ttcaaccctc tccagggtat tcacgagggt
     5881 gcagacccgg ggtggcagga caaaatcacc tcctaccctg aggtcgtacg acgcgggttg
     5941 ctggcactct tcgctatacg gctctatgac caggtctccg ctcatgtcct ctccgcgaag
     6001 gcgtctctcg atctctgatg acgacaggat cattgttaca gatcaatact acagaaaatg
     6061 cttaatatgt tgtcctgcca acgcctgtga cggatccatg gggtagagga tatcctcttg
     6121 ggcttcggac ccaaggacgc gggttcgatt cccgctgggt ccgcttttct cctctccaac
     6181 acgaccctcc cctcgcctgg cacctgatac cccctttgca tttatgacac aatcggtgaa
     6241 gggttcagag cccgcccctt cgactccgtc ccctcgcctg gccccgcttt ttcatgatga
     6301 ggaatgctcc catgaatcct gccacgacca cggccagcac tactatgaca agaacccagt
     6361 cgatgctcct ttccatggag atgataacgg gtgcggtctc attccgggac gagacggtct
     6421 tctcaagttt cacgggattg aaaccctctt tttccgctga tattacgtag ggggtgttca
     6481 gccgaaggcg gaccggaagg gtgccattct cacccgttgt tccctgctct tttccgtcga
     6541 taaagatagt ggccccatga accgggacat ctcccccctc gagggcagcg atcgagaggt
     6601 ttgcagcggg gaacgcaagg tcgacagtga tgtcctcacc ctgcacgcgt acctgcatct
     6661 gctgcctgtg ggtctcaaac ccgggatggc gtacttcgag agcatactcc ccaaacgaga
     6721 ggtttgcaag ctgcagccgc ccgtaacggt tggtggagcc gactggctgg ccgtcaagga
     6781 agaccgatgc ctcctcgaca gggtcccgct cctcgttgta gaccgagata aacacactgt
     6841 acggggactt tctgagcagg gtggtgacaa gggcctggtc cctgcccaca ttcatccgct
     6901 catgataatc gtcgtatccc tctatcctca cgtggacctg gtagaccttc tcccggggca
     6961 gggacagcgt gacaaccccc tttgcgtcgg tgatacccct ctcgacgccg tcaatgataa
     7021 ctgccgcacc ggggagcggg gccccgcttg tctcgtcctt cacaaggaca gagaaccggt
     7081 cgtcacgaac gagcattacc tggacggtct tctggtcacg cgcgatctcg aggtggtcgg
     7141 tgaaggtgcg gtaattcggg gcagttatct cgaggttgta cgccgagtcg cccgagacgg
     7201 tgaacatggt tgtaccgttt gcgtccgtga cggtcgtgtt cttcccggca ccgtcactga
     7261 tgctcacact tgcccccgcc accggtgcaa gagcgtcggt atcaaagacc tggacggtca
     7321 gaggtacctt ctttttggtc atctcaacga gcagagtcgt ggcgttgagc ccgatatcgc
     7381 cactccaggg cgcatatccc agtttttcga ccctgatctg gataatctcc gttccagggt
     7441 gggaaaattc gatgttccct ccgctatctg actttcccac gagtgcattg tttgtgtaca
     7501 gtgctgcacc cggcaggggg gtgatgttcc cttccttctc atagatattg acaaggaccg
     7561 taaccccgga ggccggccct gccataagca gcatggcgag gagtgcaaat gcaaaacagg
     7621 tcccaatcct ttgcatattc tgatatcggc gctctctgta tatcatttga ttggtaacgc
     7681 gctgcccggc tttccgaata ggaaaaaaag ggatggattt ccggattcac caggagggaa
     7741 cccgcgggtg cggcattgac agggatggct cctcctggaa gcgaaaggac cggcaggttc
     7801 agctccaccg ggtgtcgtgc ggcacccggt caaggagcat cccttcgaca agtaccggca
     7861 tcaactgccg cccttcttcg agcgctttct gcagcctcca gttcctgtca gcatagattg
     7921 tgaagagagg ctggcctttc tcgacccggc tgcctttctt tgcatggagg aggacgcccg
     7981 ccccacggtc atggggcgct cccgccgtcc tcgcgaggct aatgagggac ctgttgttca
     8041 gctcgatgac gtaccctgac gtaggggcat tgacaacgaa ctggtatttc ccgggctcga
     8101 tgtcatcaga cctgatcttc gggtctcctc cctggatctc gatgatctgg cggaattttt
     8161 tcagggcatc gccttttttg aggatctctt cggccatctg tgcccccatt ccccttgcag
     8221 ccttccctga catctcgagc gccattcccg cgatcgcgac gctcttctgc aggagagagc
     8281 ccgggctctc tccgccctca aggatccgca gcgcctccat tacttcaagg ttcggcccga
     8341 tgctccgtcc cacgggggag tccccgtatg tgagggcaca ctcaacctgc atgttgaggc
     8401 ggtccccaag ctcaatgaac tccctggcaa gtttcctccc ctcctggatt gtggccacct
     8461 tggcgtgtgt gcctaccggg atgtcgatca ccacgaggtc agcaccgacc gcatacttct
     8521 tggccatgac acttgcgagc atctgccccc tcgcatcaat gcggaaggga tactcctgga
     8581 tgatgatctt gtcatctgcg ggcgcgatgt tggtggcgcc gccccacacg atcactccgc
     8641 ccaccttctc tgtcatcctc tggacctctg atgctgagaa atcgaccggg gcgaggacct
     8701 ccatgagatc ggccgtcccg cccgcaccgg tgatagccct cgagctggtc tttgggatct
     8761 tgagaccgct cgcggcgatt atggggacca ccaggagtga gatcttgttt ccaggtaccc
     8821 ctccgattga atgcttgtcg acaattgggt gggcatgaaa cgtcagccgc tccccggtgt
     8881 caaccatcgc cctggtgagg tgctcaacct cgtccatctc catctcgttg atgtagcagg
     8941 cggtaatgta cgcggtgagc tctgcgggag agaggttgtc ggagattacg tccctgatga
     9001 ttgcataggt ctcatccttc gtgaggtgct ggccgtccat cttcttcttg atgtagtcca
     9061 gggtgaccgg cctctcggcc ttccgcacct ccacagatac tccatcttcg agctgcaggc
     9121 gttcgttggt gacctggtag atcccgatcg tccccttggg gagaagcgtg gtggtggtgt
     9181 caatgaaggc cgcaacagaa acacctgtca cctcgttgag tatctggatg cggtctcccg
     9241 ggagtactcc gatattcctc gaatcgtcga tatgcaggag gacttccctt gtaccgatat
     9301 caacgagctt gacagtgagc ttcatgtggt ccttctccgg gcaggcagcg ggtgtacccg
     9361 ttctttttgc cgttggtcct ccaggaccag ggcgaaaccg tggaatccgc gtctgatacc
     9421 aggtatgttt tcggccgtat cgtattagtg gtttctctta tttcagagcg aacgcgccga
     9481 acagattaat ggtgcgacga aaaagcctcc cttccggaga cctggcagaa aaaaggtagg
     9541 ttttggtgtt cagtgcttgc gcacgatgag gaaggccact gcaccaagtc cgatcagggc
     9601 aaccagggca ccgaatcccg gggttgtcgg ggtcggggtc ggggtcgcgg tggtcggcac
     9661 aggggttgca gcagtggtca cctgggtcgg cggggcggtg gtcggaatcg tggtcggctt
     9721 gacttcgacg acattgaaga gtgcagtcga tgtcacgtcc acggtcacgc cagagacctg
     9781 cacgatatac tcatcgggct tgaaggtggt ggtgtcgaca gggaaggacc aggtgttgaa
     9841 gccttcggtt cccttctgga cctttacagt gccagttgcg ccactgaact caccgctctg
     9901 ggtcttctgg gtcggggaga aggacgagga gacgacctca accaggatct cgtcatcgac
     9961 ggcaaggttg gtggtgccct tgatctcgaa cttctcgcct acctgcttct cggtgatcgg
    10021 ggtgatcttg atggtgggtg cctcgatcag gaactggagc ttggtgtagg tatcgtcaac
    10081 ggctgcgttg tcgagtgcca cgaccagtgc ctctgcagcg tcagatccct gcaggctgcc
    10141 gggcccctca aggaggaaga gcacggagtt ggctacgggg taaggtccga cgacttcgcg
    10201 gtagggccac ggggcaactg ctctcgggtt cacgtcgagc tggtcgttgt acatcgggtg
    10261 ctggacgacc acgaagtact ggccgggtgc catgttggcg gtgattccct ggttgatctc
    10321 cttctcgaag cttccgtcgt tgttgacgct ctcggtgtca acgcgtacgt agttcttgcc
    10381 catgatccag acctgcacac cctgcgaggg ctggccctcg gccacgccgc ggatgtagaa
    10441 cttgtcaccc tgggcgatgg tggactggga tgcgactgcg gtcacgaacg gcttcctgat
    10501 gatgatggag acggttgcgt actgggtgtc ggcgagggtg ttcttgtcag atggggttgc
    10561 cactgcgtag atggtgtagg tacccgcgtc gatgttcagg ttggcggtct gccacttgta
    10621 ttcccacgtg ttgtcgtcga ggacatccgc ggtggtgaac gaggttgtca caccattgac
    10681 gactccggtc ctcgggtcag taagcctgcc accctgcgag gggaggtttg gcccggtgat
    10741 gaagaggtac acggtgtcag tctcggagtt ggtaccggtc agcttgactt cctggcccag
    10801 gaagtagctc tgatcgccgg ccgcgacaac ggtcacggtt cccttctcga ccttcacgtc
    10861 gacttcatcg gacttgtact ggtctgcgta gagcagtgtg tcccacggtg cagggatgcc
    10921 cgctgcgcct ggtcccctct caacacggaa ggtgtacttc ttgtcctttg ttgcctggga
    10981 agtgacccat ccaacggtga tggtgccgct gttggaggtc tttgcagctg cgaagtagct
    11041 gttcgccggt gttgccggga catcctgtgc tacggtgcgt cctgcgccac cctcgtacct
    11101 gtactgcccg attgcagcac tggtgtcaac atccttctgg cttgccacaa ggagcggggg
    11161 ctggtcaagt gcggctccgc tcatctgtcc ggttcccttg gcccacagca cgtagtaggt
    11221 gttcggcctg ccggtgatgg tcaccgagaa cgggttgccg cggacgacga cgtccttgct
    11281 ggcctcgatc ttgacggtgt cagatgcgat gatcaccgtc tgggtggacg agatcgtctt
    11341 tccggtgtag tcggtgccgt caggtgcctt gtagttgtcc ttgatcttgt tcaggtcaaa
    11401 ctcggcatag acggtgtagg tgccggcctt gtacatcctt gcgccgtttg catcaaggac
    11461 atcggtgttc cagccctggt tggttacctg cggggcccag taccagaggt cgctgttggg
    11521 cgccaggccg gtaagtggac gggaggtcgt cgtggtctgg aagagtgcgg tgtaggttgc
    11581 accgtcggca gtcttcacgc ggatattgac gaatcccttg cctgctgcgt cggccctggc
    11641 ggtgacggag taggcgttgg tctcaacccg gaagttcagg tagttgcccg gcggcacgga
    11701 cttgccggtc acgtccttcc ctgcggtgtt gtcccatacc ttgacggtaa gtgcggggtc
    11761 aacgacgttg aagacggctg ctccccggac tccggtccac tggtaccatg ttccgacctt
    11821 ccccacgaag atctgcgggg aaacgtagta gctggtcgga gttcctacgg tgacgatgtt
    11881 ggtcggctga tcacctgtcg aagtcccgga tgcccagtat gcaatctggg ttgcggctcc
    11941 gagtccggca gatatgtcaa gaccctcttc accgatgaac acatctccgc ctgcagggac
    12001 gctcacgatc gtggcggctg atgcgggaag catcagtact gccagcgcga ccagagcgat
    12061 cagtgcaagg ctaaagcgct taatcatctt ttgtcctcct taactcatgt ccaaatcctg
    12121 aacgttcgaa tccaatgcac acgtcagaac cgccatcgac aggtgtcgaa cagaggttcc
    12181 accatgctcc atactccgaa taacggaata tgattcaata tttgtggaac acttaatata
    12241 tcctttttgg tcgattcagg aagaataaat cgttgagcag gcgctgaatc cggcaaagag
    12301 ttggagatct ccggttttcc acgttcaacg ccatcccaca ccatgattca tcatcgtcgc
    12361 acctgccaga ctcccttcca ttttacgcgc accagtgtat ctctgaaaac agtgagccag
    12421 attgtcacaa tattacctgg gaaacaattg ggacctgtgc agtgtacatt gggatcgata
    12481 tccaggtggc tattacgttt aacccctctc cagtacatta actagttttc cctgcgccat
    12541 attgatacat gcgtattatc aaggccccta ccctggcatc tgcccacgaa caggtggtga
    12601 ggatgatcct cgagaaagga tggaccctcg acacagagga cagggaagcc actgtcgagt
    12661 tcgaggaggt ggccatgcgg gtggaacagc caggaaaccc gcccatggtc agcccgtgtt
    12721 cacggttcca gcagcgtttc atggagcagt atgcccggga ccttatcgaa ggatccgatt
    12781 ccgtattcga gtatgattac catgaccgtc tctttaactg gggcggggga ctgatgagag
    12841 gcggcgggaa tgaggtccat gtggaccagg tcgcgtatat tgtccgcaag cttcgcgagg
    12901 ccccggcgag ccggagggca atcgccatca cctggaaccc tgcggtagat gagaaccttg
    12961 atgactgtcc ctgcctccag ctggtccagt gcgtctcgcg cgacgggatg ctgcatatga
    13021 aggtggtctt tcgcagcaac gacatgctga cagcggcggg tgcgaacatg tttgcgctcg
    13081 tctcccttca gcagatgatc gcccgttccc tggaactggg ctgcgggtct tacacccaca
    13141 tttccctcgt gccccacatc tattaccgga gggatgcaga cgatatcgag ccgttctgcg
    13201 gacacgggga gcggttccgg cctatccccg aggtctgcag ggcctgtggg aaatgcccat
    13261 gaggctgttg gcaggaaccc cgcacggtgt ttcaggtgaa tcagtcaggt taattacgga
    13321 aaagggcgaa caaagtaggg tgcgatagcc cggtagtgta gcggtcaatc atgcgggact
    13381 ctggatcccg cgacagcagt tcgaatctgc tccgggctac ttcttctctc ctgcgtaatt
    13441 gctactgctt cctgcagacc aggatggcat ggtcgcggtg atatggctcg agccagatca
    13501 catccttcac cgcaagcggg gatccttcga gagcgctgca gaagtcatcc acgacctccc
    13561 gcggactgcg ggatacatca atgctccttg tttttattgc gattactgca gctccacccg
    13621 tatcgagaaa aagcgagttc cagagagcga ttcctgcctg gtccgggtgg gcgacgtcct
    13681 ggaaaagcat ctgcaccgtc tccacgaggg gagcgtattc atgcggcttt gtggcgtctg
    13741 caaaaagggg tatgatgttc tttctccttg ccgccaccgc aagaaggtcg cgcatgggag
    13801 cgtaggcatt ctcaacggcg tatactgtct ccgcataatc ggcaacgtgt gatacggtcg
    13861 taccattggc ggcacccagg tacaggaccc tcatcgagtc ctcgatatcg atgtccccgc
    13921 cccggtagta gtatgccgca agcttgctcc ggtagggatt ccagacccgc tgcccgccga
    13981 tggtccgctc gccgtagacg cccccctccc cctctgatac gaggaccccc tccacccaga
    14041 tcatttccgg tctcctgccc tgtcgattgc ccgttctgcg gattcgagaa aaacgggatc
    14101 tgccgtaccg cggtagaaat ctatccgtgc cgctatcgcc agctttgccg caagggccct
    14161 agagaccttt cctctccgcc cctttccggc tgcatgcacc cgcctgtgct ggtagatgat
    14221 cccgtgcttt ggcgggggag tgcccgattt caggtgggag aagagcgcga ggcgtgctcc
    14281 catgacctgt atggtcgctg caggaagcct tgcaaggcgc gggagcccgc ctgccgcgga
    14341 gaggaggcgg gccgccacca cgcccccgac aaggacactg ctgtttggca tcaccttctc
    14401 cgcaagggcc gcaacatccc gcgagagcgc ggtcctgacc tctgcaaggt gagcagtttc
    14461 tgtggccact gccacgagca cagggttttt gctcccggca agcaattccc tggctcttct
    14521 gccctgcagc tcacgatact tccgggagaa ggatgggtct ttagccgcat gccattcgag
    14581 tgctctcccg gtcaggaggt tgatcgcctc gtcgatctgg tcgagcatcc ggaccatgtg
    14641 cacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncggtcag gaggttgatc
    14761 gcctcgtcga tctggtcgag catccggacc atgtgcacga gctccatctc gggcagttgg
    14821 gataccctgc gaagttcctt ctccacccgc aggagcgcga gcgatcgcag caacgcgacg
    14881 tattctcctc tcgagctgac gaaaccgcac tcccttgcaa cctcccatga aaaaggcgta
    14941 tgtcgctccc ttgtctggat gatccggtcg aggcggtctg atagaaaatc cggttcacga
    15001 gagggacatg gtgtacagac cccgccgtcc acgtctccga accagtacga taccatcaga
    15061 taatctggtg ccgggacctg tattaatctc accgggaaac atgcgccttc gataggatac
    15121 ttcagatcac cccgagacca aacgaataaa acatgacggg gaccatgatg gcccccatgc
    15181 agaagacctg tgggatattg acaaggcggg gtatgcagcc aacggctgtc gccatcacga
    15241 gcaggaacag cccgaagaga cccgcaccaa ggaagcagac aacggccacg aaggccatca
    15301 ccacccggtt cagccaggta cggtcgaccc cctcgagaag gtgtgccatc cctgccagcc
    15361 ggatggtgag aaggtaggct gccagtgcgg caagggcccc tgagaaaaga ataaaggaga
    15421 gtggggggat ctccagcagc gcgagagctg caaccacgcc gtttctcatg cggccgaccg
    15481 cgaagaatgc cgcgaggccc accaccgcgt tcatggtgtt ggcggcactg gtggcgagga
    15541 ggtaccccct ccggtcgcgg gtgtaattca ctgccgacgc gagcaccccg ttggccgttg
    15601 cattggagag ccccggaagc catcccacca cgagtccggc agcaccgcca aggacggtcc
    15661 ctttcaggat cccctctctc gagatatcaa gccccgagaa ccgctggggc gggatcgccc
    15721 ctttccccga gtagatgagc atggaaatcc caaagaggcc cgagaggagc ggcatcagca
    15781 ctgcactctc cccgaggagc gggggggcaa ggaatgaata ccggaaagtg aatatgccca
    15841 ggagccccga tgtgcagaag accagcaggc cccacccggg agcttcgctc tgcacgagaa
    15901 gaaagccgat caccgcgacc aggataatcc cgcttgccca gtcaatgact gactggaaat
    15961 gcggaatgac gagcagcagg atacaggtaa ggggaactgc aagtgcaaga ccggttgcgc
    16021 tccccagcgc cgagatcctg accgcctcct ccccccgccc ctccatgcag agggcatggg
    16081 cgggcaggac cgcaagagcc gtgtcggcat ccggtatgcc aaggaatgtg cttggtaccg
    16141 cgtcgatgaa gcagtgggtg acaagcgccg agaaaagggc cgccgcaagg aagaatggac
    16201 caaaaagggg aaggagaacc gcgagggacg caagcatgat tgcggccagc gtgttgacat
    16261 ggatcccagg caggagcccg gagaggagcc cgagtgccac gcctgccgcg gtccccgcca
    16321 ggatctcgat catcctcgcc tagggtggtg gggaattaag ataaaggaat cgaaacagat
    16381 agaacaccac tctgagaagt gccgggtgtc tcttcatgct gatcgccatc accaggttac
    16441 aggagaaaag caccgacgac caggcgcgat gcgccaggtt cggacaccgg tgcttccctg
    16501 tctccccgct gcaggccagg atcaaccagg aggagatcgc atggttcgtg ggggagacag
    16561 accggaacac ctttgactgc atcttcttca cgagcgcgct tcctgcccgc ctgatcggcc
    16621 cgcgcctctc gcgctggccg cgggtggtgg caatcgggcc ccagaccagg agggaactcg
    16681 aggcgatcgg aatcgaggcg gaggtgcttc ccaggtacta cagcagggac ttcgtggatt
    16741 accttggccc atggatagag ggaaaacaca taggcatccc ccgtgcggat gtcccaaacc
    16801 cgggactcct ctcgtccata cgggatgcag ggggaaaggc aacagaggtc gcatgctact
    16861 ccctgatccc cacaggggaa cgcctttccc tgggggacgc tgaagtgctc ctctttacga
    16921 gcgccatgtc ataccggaag gcccgctggg agaaccgcgc gggactgatc cttgcggcca
    16981 tcggcgatat cacggccggg gccatgcggg atggcgggca tcacccctct gtcgtgggag
    17041 acggttcgct tgaggggacg ctcgaggcgt tgaaccatta cctggacacc catccacggc
    17101 cggaacggac tggaaggaga tgaccccgtt tcccgtccct gaacacggcc tgctggtgct
    17161 tgacaagccc aggggccctt caagccacca ggtgactgcc tgggcgggtg agatcctcgg
    17221 gaagcgggtc ggtcacgcag ggacgctcga ccccggggtc tccggggtgc ttgtggtaat
    17281 ggtgggaaat gccgtccgcc ttgcccccat cctcctcgcg gaggagaagg agtatgtcgc
    17341 ggtcatccgc acgcatggcg atgtgcagga agacgcattg agaagcgtcg cctctgattt
    17401 ttgcgggcgc atctaccagc ggccgccccg caggagcgcg gtcgcccgca acctcaggat
    17461 acggacggta agggcgctcg aagtgcgtga gttccatgaa cggctggcag tactcggcat
    17521 cacctgcgat gccgggacct acatacggtc actctgccac cacattggac tggcccttgg
    17581 ctgcggcgca catatgcagg aactccggcg ggtgagatcg gggatttttt ctgaggaaca
    17641 ggcacacacg cttcacgacc tgaaagatgc cgcaacgctc gcgggccagg gagacccgca
    17701 gtatctcgca gggatgatcc tccctccgga gaaggttgcc cgcgggcttc ccacgcttgt
    17761 cgtccgcgac actgccgtgg acgcactctg tcatggcgca gcgctcgccg gagtcggggt
    17821 cgtccgccag gacgcgttcg cgaggggaga gatggtggcg gtgctcacgg ggaagggtga
    17881 actggtctgt atcggcgagg cgatcgtctc ctccacgcag gtgccgccgg gcagcacggg
    17941 tctcgtcgcg gcgccaagga gcgtattcat gcgcccgggc acgtacccga aaggctggaa
    18001 aaagagagag ggagcagaaa agccggagat atgagccctg gagatccccc ggcaggaagt
    18061 ctcggccccg cggagttcct gtccctcttt ccagaaggat gagatagcat taaaaaaacg
    18121 cccgatccat agtatggata cacctttttg ctgaggtagt ctagcggtac ggcgcaggcc
    18181 tggagagcct gtggggcttt tgcccctcgg gagttcaatt ctccccctca gcgtttcttt
    18241 gttctcccgt cagtgcttcc tctgccttgg ccgggaagcg actgtccttc ctccaggcgc
    18301 tgcagtgcga accggtaata ctcatccacg acctcgaacc cgaggaaacg ccgccccatc
    18361 tcctggctga ctgcggccac ggtgccggaa ccgaggaatg gatcgaggac cagatcaccc
    18421 gggctgctcg aaaacgagag gatcttccgc accagctggg gggggagttt ggtaggggtc
    18481 ttcttcttcc ctttccaata ttcgcgattg atcacccaga catcttcggg atagtgttcg
    18541 accttgtgga aagtgtagtg gccggggtct ttcacggcga agaggatatg atagtggctg
    18601 gtgacgtact ttctcctggt gaacacgccg aactggtact tccagatcag gtggttgatg
    18661 gtggaaaacc ctgcctcgtc caggctgcac agcacttctt ttaaccggtt ccaccctgaa
    18721 aaaacgtaga ggctccccgt aggggagagt acccggaacg cctcgctgat ccaccgggag
    18781 gtaaatgccc cgtactctcc tggcgggatc tcccggtacc cctcgatgac gcgcgaaccg
    18841 gtccggttat agttcatctt cctggcccgg aagtcaattg cgaagggggg gtccgtgacg
    18901 atgaggtcga tgctcccccc gcggagaagg gggaggaggg cgagggcgtc gccgtggtag
    18961 atggtgtccg gggagaacgg cccgagatcg tgcgggctca cgtgtcgctg ctcccttccc
    19021 tgcacctctc gccggcagcc cttccgagtt cgaatgcctt cctgttgatc tccaccgtct
    19081 tctttgggac caggcgctcg accgcctgga gaagggaggc gggggcaaag ggcatgaacc
    19141 atgaagcggc gccgatcatt accacgttct gcgagagggg gcttcccgcc tccaccgcaa
    19201 gggcgtctgc gtcaaccagg cagagtgcac ggcttttgag ccgctcctgc accgcctcct
    19261 tctccggaac cgctgctccc tgcatgaaga cggaagtggg gatgacaatg tggctgttta
    19321 cgaccatgtg tccttcgggc gcaagaaaat gggagtaccg gagcgcctcc agcatgtcga
    19381 acgcgatcat taactgtgcg gtgccgggcg cgatgagggg tccgaacttc ccgtcgatgc
    19441 gtatatggcc ctccaccgag cctcctcgct gggccatgcc gtgcgtctcg gcaccacgga
    19501 cattcctgcc ctcaatgagg catgcctcgc cgaggatatt cgaggcgagg atgatcccct
    19561 ggccccctat tcccaccatc aggacgtcgt agctcccgct catctcttca cctcttttcg
    19621 tattgcgcct gcagggcaga tctggacgca taccccgcac ccgctgcaca ggtcgttgac
    19681 atgcgctttc tcgccggatt tctcgatggc ggggcacccg aatttgatgc agagcccgca
    19741 tgatgtgcag ctctcggcgt cgacctggaa ccggccgcgg cgcacgccgg cccggcgggc
    19801 ggttatcacg cacggctggc gggcgatgat caccttcacg ccagggcgct cacgggcatc
    19861 cttcattacc gagagcaatg agacgaggtc gtaagggtcc acagtctcca cataagagac
    19921 tccgcatgcc ctgcagatag cctcaagaga gaccgggggg acagggacac cgaacgcatt
    19981 cgtcccgctt gaaggattcg gctgatggcc ggtcatagcc gtgatccggt tatcgagaat
    20041 cacgaggatc atcttcgccc cgttatacac cgcgttcaag agtcccggga tgcctgtatg
    20101 caggaatgtc gagtccccga tggttgcgac gacttcccgt tcttcccccg aatgggagat
    20161 gccgctcgcg acggtgacgg aggcacccat acagatggtg gtatccaccg caccaagctg
    20221 gaggcccagg gtgtaacacc cgatgtcgct tggaaatatc ccgtccttga atactttcct
    20281 cattgcgtaa aagaccgggc ggtgcatgca tcccgcgcag aggatggggg ggcggttggg
    20341 gatatcaccg gcggggacag gattgggaaa ctcccttgtc ggggcgatgc cgcaggcctg
    20401 catgaccctt gccactatga caggcgagag ttccccctcg tacggcacca tgccgtcctt
    20461 cttcccgtgc accgcgacac ctccggagac ctgccgcacg gcctcctcaa cgacggggtg
    20521 aagctcctcg attaccagga ccgactcgtg ttttctcacg aaaccgccaa gccactcctc
    20581 gtcgatggga tacgcgccta tcttcatgaa cgatacatcg ggggggagca cctcccggac
    20641 ataggccgcg gcaatcccgc tcgcgaccac ggccacggag cctcgcacct ctgcatggtt
    20701 gtaccccttc tccacaaggg ctctcttgat gtgcggctgc ttctggttca gcttgcggtg
    20761 gagtacccgg gtatgcgcgg ggatcaccac gtactgttgc gggtttttgt ggaactcccc
    20821 cttcctgggg acgctcgcgg ggcttctaac caaggtatcc cccttggaat ggcagatgcg
    20881 cgtggtgggc ctgaaaatga cgggcaggga gaactcttcc gagagggcaa atgcatccct
    20941 gaccatgtcg tgggcttcct ggatggtgga ggggtcaagg caggggagcc gggaaaacag
    21001 ggcatagcac cggctgtcct gttcgttctg ggaactgtgc gcgaacgggt cgtccgcgct
    21061 catgaccacg aggcccccgg tgacacctgt ataggcactc gtcatgagag gatccgccgc
    21121 gacgttgaga cccacatgct tcattgtgca gagcgcccgg agaccgcacc acgccgcagc
    21181 cagggcattc tcgaatgcca ccttctcgtt caccgaccat tccacgtaaa agtcccgttc
    21241 cggcagggaa cggaggatgt cgatcacctc cgatgagggc gtcccggggt acccggtcac
    21301 gaaatggaca ggtgattcca ggcacccttt tacaatcgct tcattcccaa ggagatactg
    21361 tgtcgtcatg gtcctccacc atctactctc ttctcaaaga taatctgtgg cagtacatgt
    21421 aatatagcca tcccgggtat agagacccgc gattcaaaac ctataaaagg gccggagggt
    21481 tatctattac ggcaacgcaa gggcccgtag catagcccgg tggtgcgccc ggctgataac
    21541 cgggaggtca tgtgttcgaa tcacatcggg cccattccac atcctcttat tactgatcgg
    21601 agcagagttc atactatgga aaagaaccac gttattctgg tggttttggg actgatcgcc
    21661 tgcgtagccc tctatttcct cgtcgacatc tatgcaggca tgatcggggt catactggta
    21721 atcgccctgg ccatgtccct cttcatcatg gcggacagca ctatgctgcc ggatgtatca
    21781 gtgcgtctga aagaagacgc aagggggatt atcgtgaaga accgtggaaa cgcaaaggcg
    21841 ctgtccatcc ggctctccct catcccacac gatctccatt acaccatcgc ctcactcgac
    21901 gaggatgcag agaatgtctt tccaaccgag atgatgatcg acaaggtgaa agtgattgcc
    21961 gaataccaga atgaaaaagg agacctgtac aaaaggacct tcactatctc ggcactcgac
    22021 aagccggatg atgatctcct gaaaccgatg ttccccctct tcaagtggaa gtgatcctca
    22081 ctcccacctt acttttctgc cagcacgaac gcgatggtct tttccagtgc atccaggagt
    22141 tctttctgcc cttccatgta atcacgcatc gcgcggtaca gcatcaccat gatctcgtgg
    22201 ccgtatatct ggagcgcgtc tgcgggtgag agcgggttcc ggtaggaatc gacgatgatc
    22261 ccgtctgtgc tgaacatcag ctcgtagaac tttccgtcct ccgacaggac gcagaactgg
    22321 tcatccacct tcttgctgat gttgtcgggg cggtgctcca cgggatcggt cttgccgagg
    22381 atgatcatct tctggtcatg ataccgcgtg tcatacattt ctcccttcgt atcctgcttc
    22441 ccctgcttca gcatgttcat ccctatcctg ctgaccaggg gtgcggtcat ctcccccatg
    22501 cggcccatca gttcagcttc gcgggtccgg acctcctctg atagttcctc cttttttgcg
    22561 tcgagttcgt caatcttctt taaaagtgcc ctgtatccct tctcaatctg gtccatcgct
    22621 tctgtaacct ccatttgttc aaaatctgtg ctggtatctc cggggattag atgaggagtg
    22681 ggttgcagat gcaataatgg ttccttttca tccatttcat cgtcaataaa ccatatgatg
    22741 cgatacaata tgtgggcctg acagccatga tccaaaatcg cgtgagatgg cccgatgatc
    22801 accgggccgg ttctccaatg aaataatacg tcctttcccc ggtcaggcgg acaaccatgg
    22861 tggatcctgg ggggtgattc cccctcacca ctaccccctg gtattgccgg gtccggccca
    22921 tcgatgaacc gggccggagg atctcagtaa tgagaacgag gacctccttt cctatccatg
    22981 cctcgttcct ggacctcgca attttctccg catgggcccg caggatccgg gacctttcct
    23041 tcttgaagcg gtctggcatg tccttatgcc tcgctgcggg agttttcggc cggggagagt
    23101 aacgggtcac attcaccttg tcaggctgca tccgtgagag gaatgccagt gtctcgcgga
    23161 actcctcctc ggtctccccg ggatagccgc agatgacgtc ggtgtggaga gtgatcccgg
    23221 ggcagtgtgt gcggaaccgc tctactattg cgagtgccct ggcagcggtg tatcccctcc
    23281 ccatcctctc aaggaccgca tccgacccgg actggaccgg gaggtggaaa aacgagaaga
    23341 tcctctccct cccgaacgct ccggccacct gatccatgac gggaaacagg gtcgccgggt
    23401 tcatcatccc cacgcggacc cggaaattgc ccggaacctc gccgatttct gcgagaagca
    23461 tcccgaggtt cgacccggag tccatgcccc atgcgctcac gtcctggccg caaagccgga
    23521 tctccaccgc cccctgcccg gccagagact ggacctcgcg gaggatctcg tcggttccgt
    23581 aacttaccag ggggccgcgt gccctgcggg tgatgcagta cgagcacgaa cccctgcacc
    23641 cccggcatat ctgcacgatc cccacccggc ccgatggcat gcatcccggt ggcgcatacg
    23701 acgcatgtat ctcatcgggg tggaagatga cgggatcaca ggccgagagt atggcatccc
    23761 tctgcaccac ggccatgcac cccgtgacat agagggagtg gttccgcagt gaacgaagct
    23821 ccctctgcac tttccgttca gtcgcaccga tgaccgtgca ggtgttcaca atgacggccc
    23881 cggcctcttc cgggctgctc acgagcgtgc aaccctgccg ttccagcact gccgcgatct
    23941 tccggctgtc cccctcgttg taggtgcaac cgaatgtctt gatatatacc ttcttttgtg
    24001 cgattgtgcg caataccccc tgttcagggg gatggccgtc tcgggagcct tccatgcctc
    24061 tctcatacac tctccgccag agcagatgag ggttcacatc ccgtccaggc gctcaatccg
    24121 catcctgcga taccccggat ctatcccaaa aaaaaaatac gtcgaatcct taagaaccac
    24181 agatgaatat cccgatggat ggaaaaatgc ttaaccggtg gcattcccaa tctctagtac
    24241 aatgatcagg ataggggtgc ttggttgcgg aaatatcggc cacatcatcg cgaaataccg
    24301 tgaaaacttt gagatagtgg ctttgtatga ccaggtgtac ccaagagccc tcgaacttgc
    24361 cgcactctcc ggagggaacg cataccagga cttcgaatca ttcatcgcca gtgatttcga
    24421 gtatgtcgta gaggccgcct ctgtccgggc cgtgcgccag tatgcggaga tggtgctgtc
    24481 cggagaaaaa cacctcatcc tcctctccgt gggagccctt gccgacccca acttccgcga
    24541 agacctcgtc cgcatcgctg aagcggcagg gaggcgcatc tatatcccaa gcggcgcagt
    24601 cgcggggctc gacaacctca aagttggcca gatctctccc atccaccgcc ttcttctcag
    24661 gacgacaaag agccccgagt cgctcggcat acggacctcg gaacgctgcc tcgtctttca
    24721 gggcaagtcc cacgagtgca tcaaggagtt cccaaagaac gtgaacgtct cggtggcatt
    24781 gagccttgct gcgggaagag acgccgacgt cgagttgtat gctgacccat cagtcgagag
    24841 gaatatccac gagatcgagg cagagggaga gttcggagag atctacatac gggtaagcaa
    24901 tgtgccgagc ccggataacc ctgccaccag ctaccttgcc gccctctcta tcctcactct
    24961 cctccggaac ctcgagcagc ccctggtggt cgggacatga gcgtaaaagg acgcataatc
    25021 tcactgaaaa atcagaagaa tgccatcata ctggcgcata actatcagcg gcccgagata
    25081 caggatctcg ccgacgtggt gggtgacagt ctcgagctcg cccagaaagc aagggaaaca
    25141 gactccccgc tcattgtctt ctgcggggtc ctcttcatgg cagagaccgc caagatcatc
    25201 aatcccggaa agaaagtgat catcccggag aaggatgcca cctgcccgct ggcagaccag
    25261 ctctctccct cgctcatcct cgaggcaaag gagcgccacc ccggtgcacc tgtagtcctc
    25321 tacatcaaca gcactgcgga atgcaaggcc catgcagatg tgatctgcac ctctgcaaac
    25381 gcagtgaagg tggtccgatc ccttcccgag agggaggtca tcgtcgggcc tgattccaat
    25441 ctggcagcct acatccaggg ccagatcccc gagaaagaga tcatcgcgat tcctcctggc
    25501 gggcactgct acgtgcatga gtgtttcacc tatgaggatc tcgaatccgc ccggaagcgc
    25561 gggggtgaga tcatctgcca ccccgagtgc cgtcccgaga tccagcgtgg atcggacatg
    25621 gtggcctcca ccgggggtat ggtcaggctt gtcggcagcc ggaagggcaa cgcagcctgg
    25681 aacatcctga ccgagagaga gatggcgtac cggttaaaga cgctttaccc gggacacact
    25741 ttccatgtca aggaggatgc catatgcgaa gatatgaaga agaccaccct cgagaacctc
    25801 ctcctggcac tcgagtacga acagtacgag gtcaccctcc ctccggacat catggaccgg
    25861 gccaggggag ctatcgagag gatgctggtg atcggccggt gacgaagggt cccgagattc
    25921 ccctgggcct gctgctctct ttcgttcggg aagatgtacc tgccggtgac gtcacctcgg
    25981 actcccttct ctccggccag cgctgcactg ccaggatcgt ggcaagggag gaaggggtca
    26041 tcgcgggact ggaggaggcg tcagcccttt tccaggcgca tggagtaatg gtttctccac
    26101 ggctcggcga cggggagccg gtggatgcag gggcagtcat tgcaaccctc gaagggcctg
    26161 cgagagcagt gctccttgta gagaggaccg cgctcaacat cctcgggagg atgagcggaa
    26221 tcgcgacgat gacacgccgg ctccaggtga tccttgaccg cgttggaacc ggttgccgga
    26281 tcgcatccac ccgcaagacg gcgccggggc tccgcatcct tgacaagaaa gccgtcattc
    26341 tcggcggggg cgacccccac cggatgaccc tctccgacgg gattttaata aaggacaacc
    26401 acctggaact ggtgccactg gaggacgcgg tgctgaaagc aaaggcatac tcgaggtaca
    26461 ggaaggtgga gatagaggtg cagacccctg acgaggcact catggctgcc cgggccgggg
    26521 cggacatcct gctccttgac aatatgtctc ccgcggtgat cagggagtgt ctctcccggc
    26581 tggagagcgc aggcctccgc gagggcctcc ttgtcgaggt ctctggcggg gtcacggaag
    26641 agaacctccc gggctacgcg atggaagggg tcgacttaat cagcatgggc gccctcaccc
    26701 acagcgtaaa aaatctcgac gtgagcctgg aaatcattca tcagtgaccg cactccgcac
    26761 ttttttgacg accgccccgc accagcgaga tcacagtgct atcatgtatc atccataagt
    26821 gatcggaaac acaaaaaaaa ggatctggag ttctgtgccc tggaagggag ggatccgggt
    26881 tttatcccac cacattcccg gtgttatttg aatatgtcga aaatctggtc gcttccggtc
    26941 gcaaggagcc tgtctctctc ttccctctct tcaacgaggg caaggagcgg caatgacatg
    27001 tcctgcccgg ggacgtgacc aaacatcttc tcgagctcca cctggttggc gtgcatgaac
    27061 agcgcgttcg ggatctccat cgggcagttc tcctcgcact gtccacagtt cacgcaactg
    27121 tcggcaatgt gcgagaaacg gatgaggtgg aacatgaaac tgacagggag ttcaccgggc
    27181 ggcacgaagt atggcttctt ggtgctgcac tccacgcagt agcagatggg gcactgctcg
    27241 atacatgcgt aacatttgac acaccgggac gtcgcgtctt taattttctt gaggcggtct
    27301 cggccgttgc ccagcccttc gaagtctttc ttcctccact tgtcggcgag cttgaacatg
    27361 gcgttttcga ctttgccacg tatctccagg cctttcgggt tcggtgcctc tgtcttgatg
    27421 acccctgcac cgactgccgc cttgaggaga ccggcaccct tctcgctgca gacttcaacg
    27481 aaggttgcct tgccggcctt gtcgccaata accccccagt tcccgcaggc gagatcggcc
    27541 tgtcgtggga ccttcatctt gcagcgccgg cagttgctcc ggcggccgta cccctcttct
    27601 tccagttcat cgatcgagat ccccttgtgc cctccctcgt attcgatgat gaactggccc
    27661 ttgtcgatct cttccttgtg caccttgtcc gggtcgacgc cgaatttttc cttgatcatc
    27721 ttccgggcga ccacagggct caccgagccg ccgcagttga ccccgatcat caggatattg
    27781 tcaaggttca cctgctggcg cttggcaagt tcgtagaggg ccatggcatc gcatcccttg
    27841 acagtcatgg cgatcttcat cttgttcgcc ccgtcaaggt acttcttcac aagtttcgag
    27901 acgagcagcg tcccacagtg gagggagcct gccgtctggt ccagctcttt tggatcggtg
    27961 atgattacag gtactgcatc atacaggtcc tggcctttct tcaccgcaag cactgcatca
    28021 accgctttgc tctcgagggc aaacttcagg agctgggtca ccgcgccgcc gcactcggcc
    28081 ttcttggcaa ggttggcatc agtggtccat gcatagaaca tgtcgccttt ctttaccatg
    28141 gtttagttcc cctccttggt cttctcgaca gccacagcac agtgcttcag ctcaggcatc
    28201 tttgacagcg ggtcaagcgc ggtgttggtg agggcgtttg ctccctgggg gaaatgcatg
    28261 gccatgaaca gcacgccagg tgcgacctcg tctgttacgc gggcgatcgg gagtgattcg
    28321 ccacgccggc tccgtacctt tattttctcg ccgttggcaa tcttcagcct ctttgcatcc
    28381 tcggtattga tctgcacgta attctcaggg acttcgtaat ggaggacctt ggcccggtcc
    28441 gtctgtgtcc tggtgtggta gtggaagatc aaccgccctg tcatcagggt gaacggatac
    28501 tcggcatccg cgacctctgc gggaggccgg tactcaaggc cgaagaaggt tccaagtccg
    28561 tctgccgctg cgaacttctc gcggtgcagg ataggggttc ccgggtgatc aagggtcggg
    28621 cagggccaat ggagcgactc cggcttctcc agccgctcgt aactgcaccc ggacatggac
    28681 ggggtgacag cccgcatgtc gtcccagata tccttcggag acttgaaatc gaagcccttg
    28741 aggccgagct tcttggccag caggacgaag atctcccagt cctccttgga atctccgggc
    28801 gtgtcaaccg ctttccggat gcggttgatc cgccgctccc cgcttgtgaa cgtgccgtcc
    28861 ttctcagcaa acgagcagcc cggcaggact acatccgcat gttcgcatgt ctcggtccag
    28921 aagatgtcct gtacaaccag gaaatcgagc ttgtccaagg atcgcatcac gtggttcgag
    28981 tcagggtaag agaccacagg gttcagggca aagatataca ttgccttgat gggatcgccg
    29041 cactggttga tctgttcagt cagggtcgag ccgtaccagt cagggagaga ccccggcttt
    29101 aatccccatg cctcctccat cttcttccgg gcctccggaa gttcgcactt ctggtacccg
    29161 gaatacacgt tcgggtatgc acccatgtcg caggcgccct ggacattgtt ctggccgcgg
    29221 agcgggttta ctccgacacc ttcgcgccct acattgccag tgagcatcga gaggttgccc
    29281 atcgaacgga cgttgtcggt accggtggtg agctcggtga tgccgaggca gtaaatgatt
    29341 actgcattct ttgccttcgc gtatttccgg gcaatatcct tgactttctc gagcgggaca
    29401 ccgtgtatgt tctccacatc ggcatacttt tcgaccatct tcttgaggtc ttcgaatccc
    29461 tttgtccgct tctggatgaa ctccttgtcc tcaaggccct ccttgatgat ccagtacatc
    29521 atggaattgg caagagcaat gtgagtggat gggttgaagc ggacccagtc gtctgcaagg
    29581 cgggctgtcg gtgagtacct tggatcgatg acgataatcg gggttccctt ctgctttgcc
    29641 tgggctaccc ggcgtccggc aagcgggtgg gcctcaactg cgttcgaacc ccacatgaga
    29701 atgaggtccg agttgagtac atcctcgaac gggttcgtgg ccgctcccga tccaaaggag
    29761 agagaaagcc ccgcaacaga tggtccgtga cagatccttg cacagttgtc cacgttgttt
    29821 gtgaggaacc caacccgggc gagtttctgc ataatatagc actcttcgtt cggagtgcgg
    29881 caggagacct ggaacccgag agacttcggc ccatgcttgt cgtaggtctc ctttaatttt
    29941 tttgcaacaa ggtccagcgc ctcgtcccac gaggcctcca cgaatttgcc atctttcttg
    30001 atgagaggct ttgtcaggcg gtccgggctc tggacaaact cccagcacgt catccccttt
    30061 gggcagagtt tgccttcatt gatcggagat ctcctgtagg gttcgacccc cacgagtttc
    30121 ccctcgctga ccacgaggtt cagcgtgcat ccgacaccgc aatacgggca tgtggtctgc
    30181 acgtatttca tctttgatga ggttatgtca ctcatgtcgt tttctccagg aattcaaagg
    30241 ctgttcaaca ttcaaagttg attttttcac taaattgagt gtattcaatg aatttggtaa
    30301 atatgttaat aaaattaatt atttaatggc aatttttgct gaatattggt gggcaattca
    30361 gataccgcga cgtgcggaag ctccggcttt ttcctccgga aagcctcccc tggtatatgt
    30421 gggccggaaa aacacgatgg agatgatgac aatccatgga caacgcccct ggaattcccg
    30481 gatcacgggt gaaatgtggc gatcaaaaaa aagttagttg gtgtaggccg atttcccgag
    30541 ctttggctcg aagaacttgt ccgcctcggt ccggattgcg tggctgaaga gcgcgagcgg
    30601 tatctccgcc gggcagcatt cctcgcactg cccgcagttg atgcaggagt ccgagatgtg
    30661 cgcaaaccgc ctgaggtgga acatcgggtc ggccgggacg aacccggcgc ggaccatcag
    30721 gtctggctgg tcgaacttgt cgggcctggt gatgcagacc gggcagtggt cgatgcacgc
    30781 gtaacacttc atgcaccgtc cggtctcctc ggcaatgctc tcccagagtt tcggggcaag
    30841 tgcctcgaag tcgcgcttcc tccacttgtc gccgagcttt aacatcgcat tctcagtctt
    30901 ggccctgatc tcgatgcctt tgggatcggc agcttccgtg gacagtttct tcgcatcgac
    30961 tgctttcgtc agcagggcag cgcctttctc gctgcagacc tcgacgaagg tggccttccc
    31021 ggccttatcg ccgatgactc cccagttccc gcaggcaaga tccgcctggc gggtgatttt
    31081 caccttgcag cggcggcagt tgctgcggcg gccgtatcct tcgtcctcga ggtcgtccat
    31141 cttgatgccc ttgtggcctc cctcgtactc gatgatgaac tggcccttgt cgatctcttc
    31201 cttggtgacc ttgtccgggt caacgccgaa tttctccttg atcatcttcc ttgcggcgac
    31261 cgggctgacc gatccaccgc agttgacgcc gatcatgaag atgttgtcga ggttgacaag
    31321 cttgcgcttc gcaagctcga gcatgcccat catgtcgcag cccttgacaa ccatcccgat
    31381 cttcttcccc tcgagtttcg gcgcgtattc catcaccagc ttcgagagga ggagggtacc
    31441 gcagtggagt gacccggcac aattgtcgag ttctttcggg ttggtcacga taaccgggat
    31501 cgcttcgtat atgtcgacgc cccgggttac ggcaagcact gcgtcaacgg tcttgctctc
    31561 gagtgcatac ttaaggagct ccgtcactgc gccaccgcat tccgccttct ttgcaagggc
    31621 ggagtcgttg gtccatgcat agagcatatc gcctttcttt gccatctact tcgcccccgc
    31681 tatcttttcg atctttatcg cacagtgctt cagctccggc atcttggaga gcgggtcaag
    31741 cgcggtattc gtgagcgtgt ttgcaccctt gtggaagtgc atggacatgt acgtcacacc
    31801 gggcgcaaca tcgtcggata tacgtgcgag ggtctcgatc tgcccgcgac ggcttgatac
    31861 cttgatcttc tcgttttcct tgatgcccag tttctttgca tcgatggtgt tgatctggat
    31921 gtatgactcc ggcacctcgt agtgcagctg cttggcacga tcggtctggg tccttgtgtg
    31981 gtagtggaag atgagacgcc ctgtcatcag ggtgaacgga tactcagcgt cggccacctc
    32041 cgcaggctgg cggtactcga gaccgaacat ggtcccctta ccgtcggctg ccgagaactt
    32101 cccgatgtgg aggatcgggg tgccagggtg gtcgaccgcc gggcagggcc agtggatgga
    32161 ctcgggcttc ccgagccttt cataggtgca cccgaacatg ttcggggtgc atgcccgcat
    32221 gtcgtcccat acatcctcgg cagtcttcca gtcaaagccc ttcaggccca tcttcttggc
    32281 gaccatcccg atgatctccc agtcgtactt cgactcgccc ggtccgtcga cagcttttcg
    32341 tacacggttg atacggcgct cgccgctggt gaatgtcccg tccttctcgg caaagcatgt
    32401 gcctggcagg acgacatccg catactcgca gctctcgttc cagaagatgt cctgcatcac
    32461 gcagaaatcg agcttttcga gctgcctcat gacatggttc gaatcgggat acgagaccac
    32521 cgggttcagt cccatgaagt acatcgcctt gaccgggttt ccggcctcgt tgatctgttc
    32581 ggtaagggta gagccgtacc agtcggggag ggagcctgcc ggcattcccc aggccttctc
    32641 catcttcgca cggttgtcgg caaccgcgac ggcctggtac ccggagtaga cgttcgggta
    32701 cgcacccatg tcgcaggcgc cctgcacgtt gttctggccg cggagcgggt tgactccgac
    32761 tccggggcgg ccgacgttcc cggtcacgag cgcaaggttg cccatcgaac ggacgttgtc
    32821 ggtaccggtg gtcagctcgg tgatgccaag acagtagatg atgaccgcgt tctttgccct
    32881 ggcatacttg cgggcgaagt ccttgacctt ctcgagcggg acgccgtgga tctcctcggc
    32941 atcggcatac ttctcgacgg tcttcttgag gtcgtcgaat cccttggtgc gctccgcgat
    33001 gaatgccttg ttctcgaggc cttccttgat aatccagtac atgatggagt tcgcgagcgc
    33061 gatgtgggtc gatgggttga accggatcca ctcgtcaccg aggcgtgcgg tcgcgctgta
    33121 ccgggggtcc agtacgatga ccgggattcc cttcttcttg gcctgtatga cgcggcgacc
    33181 ggcaagcggg tgggcctcga atgcgttgga accccacatt acgatcaggt cggcgttgag
    33241 gacatcctcg aacgggttgg tcgccgcacc ggacccgaac gagagcgaga gccctgcgac
    33301 agagggtccg tggcagatac gtgcgcagtt gtccacgttg ttggtcttga aaccgacacg
    33361 ggcgaacttc tgcagtgcgt aacagtcctc gttcacggta cggcaggagc tctggaagcc
    33421 gagggctttc gggccgtgct tgtcagagat ctccttgaac ttctttgcga ccaggtcgat
    33481 agcctcgtcc caggtcgcgt ccacgaactt gccgttcttc ttgatcttcg gcttggccag
    33541 ccggtcaggg ttgtggacat gctcccagca ggtcattccc ttcgggcaga gcttcccctc
    33601 gttgatcggg cttcgcttgt tgggctctac cccgaccacc tttccatcgt tcaccaccag
    33661 gtacagactg caccctacac cgcagtaggg gcacgtggtg ggaacataat tgatgactgg
    33721 ttcagatgct tttgccattg tcttattctc cttttctgtt ccagatcctt ctcctcgtaa
    33781 gaaagacagg agtttcattc gaatcgcctg ttatgtactg ttcttttgga aatagttctt
    33841 aaagtcaggt tgctacgtgg agaccaaatc tgaatcgtcc cgtccatacc ccttccagcc
    33901 ttggcatgcc aatccatgat aaagcaggtc gtaacgcagg acaagggcat caaatcccct
    33961 gacaatctgt ggtatagatt aactggagaa aaaattaact attttaagtt tatgcgcact
    34021 acattcataa aaaaacaatt atatcaattg gatttaaaaa atttgtttga tttattgaat
    34081 ttattttttt tacaatatct gcattataaa aaattttaat gggttttgac ttatatgcgc
    34141 gaaaagagtc cggattattg cgcggaaata atgtaaagtt tacattatta acatgaacca
    34201 gatgttgcac atttaaagtg ttttccgcac acatggtacc ctatgcctac cataccggaa
    34261 tatactccag gcgaactgct cgcgaagatg gaatcgatgg ggatccccga gtctcttcgt
    34321 gaatcattca ggaaatgcat agacttccac acatatgccg cccccggcat gctgatcggg
    34381 gtcttcatgg tcgattgcgc tctcaagatt ctcggcagaa gacctgaaga aaagatattt
    34441 gttacctgcg aaacgcacaa gtgcctcccg gacccaccgc aggtcatcat gcatgccacg
    34501 actgggaacc accagctccg ggtccttcct atcgggaaat ttgccatgac tttcactccc
    34561 tcttcggtaa aagagtatgc tgaaggtatc cgcatctacg tggacaggaa gaagcttgac
    34621 cagtaccctg ctttggcttc ctggtatgac aacagcgctg cgttcaaacc tgcaaccatg
    34681 aagcggcagc ttgccgaaga gatcctggcc gccgcctgcg ggatgttctc ccatgagagg
    34741 gtaagaatga aggtcaccca caagaaaaag tggaattccg tcacatgccc ttcctgcggc
    34801 gaacaggtcc cacaagacct tatggagggc accacctgtg cgggttgtgg cagcctggct
    34861 tattatgaga aagtaacctg actccaccac ggcaatccac ttcacacagg ggaacgcata
    34921 gggtgcatgg gaaagggaaa cgggatatgg acaattcgct ggtgctcaca cccgtaggtg
    34981 tggtccattc tcccgtgcat gaaagccgaa taatgcctgt ccagggtgtg agaggacaga
    35041 ttgaaatttt tccggagttt gtccctgctc tcgagggcgt ggagaccagt tcgcacctga
    35101 tacttcttgc ctggatgcat cgcggagagc gtgacctgct cacggcgaga gcacggaaaa
    35161 tatcagaaga tctccctctc aaaggcgtat tctccctccg atccccttcc cggccgaacc
    35221 ctctctctgt gagcgtggta agactcctgt gcagaaagga gggcggaata ctcgaagtcg
    35281 actccctgga ctgcatcgag ggcactcccg tgatcgacat caagccctac cagccgggat
    35341 gggactgcgt attttcggcc acccaccacg accggtcggt gaagatagag aagatgggcc
    35401 catctgaata ccgccagatg ctcatccgcg aagcggtgaa ttaccacggc gaatggtgcc
    35461 caggggccgc aattgcggtg cgggttgcag agtgtgcaac ccgcgccctt ggcggggatc
    35521 tcacccgtcc cgaagtgcat ctcttttgcg ggggtcaccc gtgcatcacg gactcgctta
    35581 tcgggatcac cgcggcaagg aggggaaacg gtcggttgca ccccgtcgcc cgggaggaaa
    35641 accggggcct ggaatgctgt gaattttcta taaagagata cagagtcgtt ttttccatat
    35701 cacgactgcc ggaggatatc ccgtacatcc tctcctgcag aaatgaagat ctcttctcca
    35761 ccagagtatt cacgacctga ttcaagatcc atcagcccag gagaatccgc tccgggacac
    35821 tgtagatatt catcctgggg gggcggacga acccggcaag ggtcaacttc actctcctcg
    35881 ccaggtcgat gcctgctgcc gaaggggcat tattggtgac gacgattggg attttcgcat
    35941 ggtagacttt gctcaccatc ccttcgggca tccttcccga acagcagaga aacgtctttc
    36001 caggatcaat ccccgacagc agcgcttttc ctatcgcctt gtcaacggaa ttgtgccttc
    36061 ccacgtcctc tgcatgggat accgccgtgc cggtggtatc atagaggacg gtgcagtggg
    36121 tacctcccgt ctcccgccag agcaccgcgt gatcgttgag gcattccatt gcgcaataaa
    36181 taacactcct ggacacccgc gcagcgattg gaaggggcac cctcatagtg tgctcgtcct
    36241 ggatgacccc cacaccgccg gaggtccgga tctcctttgg ccgggcaaga atttcccttt
    36301 cgatggccgg ggcagcaacg tgaatcctat ccccctccac ctccacctcg ctgatggcaa
    36361 aaattccagg aacgatccct tcagtcacac agtagccaca tgcgaattct gcgagttcgc
    36421 ggggactcac cgtcagggat gcgatatgac ttccattcac ctcaaggtgc acccgctctt
    36481 ccctgcagac ctgcaccctg cggggtccat gaccgtccgc atcaacggag atcacatcgt
    36541 attcggaaat catcggcccg gaatcgcatt cattctcaaa gatcttcaat actcacgacc
    36601 ctccctcttt tcatgcaatg aatcagaatg gtgcgcgccc attataattc tctttaagtt
    36661 aagtactttc ttcgcaagat acacttttat aagttaacaa tggtaaattg ataaaaattg
    36721 gttttcgaat gaaataatta attataattg atttggtgac tttttacgca tgaaaaagaa
    36781 tctgtttcgc gtaacgccgg tcaccctcgg aacccttgta tgcattctga tgttccttgg
    36841 tgcagtgacg ccgggatgta tcacgtccaa ggagaccacc cccactcctg gagtttcgca
    36901 ggcgggcatc cttgtatatt ccggggcagg acttaaatct gcgatgactg atattggcca
    36961 ggaattcacc cggaaatacg gtattgaggt ccagtataat tatgcaggaa gcggtgttca
    37021 catcagccag atgaacctta caagaaaagg tgatgtattt atcacggggt ccacggttga
    37081 gtataataac gcgaagaacc agggtcttgt gggcccatat cagctggttg cctatcatgt
    37141 tcccgtaatt gccgtgcaga aaggaaacga aaagaacatc acgatgctcc aggactttgc
    37201 caggccgggc ctcaagattg ccctcggtga cgtgaatgca accgccattg ggaaagcagg
    37261 agcgaagatg ttcaagaaac tcaatatcac cgatgcagtt gaggagaatg tcgtgaccag
    37321 gaccccgacc atcaatgaac tgaccattct tatgaccgcc ggccaggcag acgcttcgat
    37381 cctcactctt gacctgataa acgacaagac aatggaggca atacagattc ccataaccgt
    37441 gaatgaagtg atgatcatcc ccgtcggcat cacgaccttt tcggaaaacc aggataatgc
    37501 caggaaattt gcagatttcg tggcatccga tgaggggaag gcgatcttcg tttcgcacgg
    37561 ttttccgaaa tatcctgatc cggcatatgc ggatgtgaag ccataaagag ggaagaagac
    37621 tttggtgaat gtatccatgt tgatagaata cgcacaaatc ggtgttgccc attccccctt
    37681 ccgcaatgca ggagcacccc cggtgcacag cacgttcacg caggcgaagg ggaccatcga
    37741 gatcttcccc gaataccggg aagggctcgg gagcctggag ggcttctccc acctcatcgt
    37801 catctatcat tttgaccgtg cggagcggcg ggctctgaaa gagcctccgc ttgtagatgg
    37861 ggagacaccc catggaattt ttgccacccg ccatatcaac cgcccgaacc caatcgggat
    37921 ctcctacgtg caactggaaa agatcactga cgggatactc atcgtcaagg gtatcgacct
    37981 ccttgatggc acacccattc ttgatatcaa gccctatatc cccgcattcg acagtatacc
    38041 cgatgctacc aacggatggg tgacccccca gcatatcgag aagatccgaa acgtaagtct
    38101 ccttgccaca agggataatg acaccatgca gtaacagcag gacgcaaccg ggataccgcc
    38161 gatgaagaga ttctcgctca tactcaccta cctggtcctg gtcgccatca gcgcagtcct
    38221 ccttgggctt gtcacatatt ctccgcttcc cgatctcgtg gccagttttg cgagtccgga
    38281 gatacagttt gcgatcgtcc tcagtctcgt cacgagcttt gcatcaacgg ccatttgcat
    38341 cgtcatcgca attcctgtcg cgtatgctct ggcccgattc gacttcttcg ggaaaaggat
    38401 cgcaaacctc gccctcacgc tccccctcac cctccctccg ctggttgcag gaattgccct
    38461 cctgctcttc tttggaacga cgccgtgggg aaaagctctt gagcaggccg gattcatggt
    38521 cattttcact ccgcttggta tcatcgtggc agaagtattc gtgaacatcc cctacatgat
    38581 ccggatcctg cggtctgcgt tctccacgat caatccccgg tacgaatatg ttgcaaagac
    38641 cctcggctgc acggacaccg gggcgttctt ccgtgtcacc ctgccgatgg cccgatcagg
    38701 cctccttgcc gggaccgtca tcacctggtc aaaggccatg ggggagttcg gtgcagtcct
    38761 gatggttgcc ggtgcaacga caatgcggac agagacgctg ccgatagccc tctacctcaa
    38821 tatctcaaca ggggacctgg atctcgcagt tgcagccgcg accatcctga tcctgatctc
    38881 gcttgtgacc ctgtgtgcgg tggaatattt cgacaaagga gtcaatgtct tttaggaggt
    38941 gaggggtaat gctgcgcatc gattcggtct caatcaccct cggcgagttc tctgttcgtg
    39001 atgtgtcact cgagatacga cccaatgaat actttatcat tctcggaccg accggtgcgg
    39061 gtaaaacagt gctgcttgag acaatcgccg gaattcatac ccctggctca gggaagatct
    39121 tccttggcaa ccaggagatc acctccatag aaccgcgttt gcggaatata gggatggtct
    39181 accaggactt catgctcttt ccccatctca ccgttcggga gaatatctcg tttgggttga
    39241 aacagagaaa acttcctgtg gatgagcaat ccacgatggt ggatgagatt tgcgccttgc
    39301 tcgagatccc gcacctcgcc gggaggtacc cgggttcgct ttcaggaggg gagcaacagc
    39361 gtgtcgccct tgcccgtgcc ctggtactca agccggaggt acttcttctc gacgagccca
    39421 tgagtgcgct cgatcgccgc acgagagagc ggatgcggac tgagctctcc cgtatccgga
    39481 agctcactgg gacgacgatt gtccagatca cccatcattt cgacgatgtc tttgcgattg
    39541 cagacagaat agcaatcatg cgggaggggc ggatcgtcca ggtcggtgag actgcagatg
    39601 tattcctgca tcctgccgac acgttcgtcg cagaattcct cggcattggc aacataatcc
    39661 ggggcacgtc atcacgggca ggatatatta ccgtgataac tcccgatacc ggtccggtat
    39721 tctatgcctc ttcaagagtt tcaggcgatg tggtcgccac tctccatgcc gaggatgtga
    39781 tcgtctcggt ggccccgttc gcttccagtg cccgcaactg cctgtatgga accgtttcag
    39841 agattcttgc gtttggaagc acgatacggg ttatcatcga cgtgggattc ccgctcacgg
    39901 cgctccttac cagggagagc tgccacgaac tgcaaataga gccgggaagc agggtgtacg
    39961 caaccttcaa ggcaaccgca gtacatgtga ttccggttaa tccggaataa gtaaatcagc
    40021 atgtaccaat aaaaacgaaa aaaccgcatc ccttaccacg acagcattga tgcgttgcta
    40081 tcaaatggtt gacggatgta aatgtccccc gccagaaaaa tagtctatac cagggatgct
    40141 cccctgcggg catacatcag ttttcgcaca ccctcgacct gctggaaagt cgcaccctgt
    40201 ccgaccgtcc gcatcaccgc ggcctcgtcg atgatctgtg aaccggcaat gatagacacc
    40261 ccgtaatcga acattacttc tgagaggggc gtgctcggcc cgagaaccat cacaagagcg
    40321 tccgggcggc atagggagag aagatcctcc agggtatggt ttatcagcgc acttccggtg
    40381 agtgcgacga tatctgcctg cgggatgaga tccgcagcgg tgttcgcggg ataatcacct
    40441 tcagcaggct gctgctcgat cacccagaga tttcctacgc gccttcgaac ctggggtata
    40501 aacggaaaat gccccacgag ggccacgttt tttccggcac ccttctttat cagcacctcg
    40561 gatgcgttca tctcaactgc gtccttttgg ttcataaaaa gaagggagtt gattgccgca
    40621 acgccgatgc ttgcttccag gcggtttttc gagcgagaat attcggccag ctcctgtgca
    40681 gtcttctggt gcagattccc gacgttgcgg acaaaagttg catggtcatg gggtttattc
    40741 gcgatgatgg tcgtggcaag accgcaatgc cggctgcata ctgcagtcca gtgcaccccg
    40801 acgagaaccg aacgaaccgg gactggatcc tccgggagcg tcgcgagcag atcgtcaaga
    40861 atgtgaaagt cgctcatggt attacctcac tcctctctcc ggcaccagga agaaatggtt
    40921 gcggcatgag gaagcatgtt acaggatatt ggaattgatt acataagagg gtaaaggtaa
    40981 ttacattcac tcagtctatc ttggtatcaa gactcacaga cggattacag agaactccaa
    41041 aatcgcactt tacaaatgac ctgtcatgtc ggtgcctgta aatcacgcaa cagactgccc
    41101 catgtgttca ttagatggcc gggccttgaa gagtactcat tgtaagaatt aaagagatat
    41161 acgtactttt ctctaaaatt aaatacaagc agtcaacaca tggatgagag aaccgcatgc
    41221 cgtccaagtc tgaaatgatc cgcatcaacc aacgcatcgc acgaagcggc ggggtcatat
    41281 gtgagagaga cccccgcttt tcgaccgtcc gcctgagatt tgcagggggc atcgcaaccc
    41341 gtgaacatct cctcgggctc gcgagagttg tccgcaggct tggattgggg ccgctccatc
    41401 tcacgacccg gcagactgtt gagattcctc atgtggagaa tgagaaggta ccctccctcc
    41461 tgctttccct ggagaagatt gggattgcac tgggggccga gcacgatgaa gttgtcaata
    41521 tcaccacgtg tcccgggacc gaacgctgcc ggctggcaaa cattgcgacc gggacgctgc
    41581 gcgaggatct ggacagggtt cattttggca aacaaatgcc tattcgcgtg aggatcgcag
    41641 tctccgcatg tccgaacgga tgtaccagcg aacgcctgag cgagatcggg ataactggtc
    41701 ttcgaacccc aatcaggaac gaggggaaat gcacggggtg cggcacgtgc gcgaatacat
    41761 gcaaagagaa tgcaatcgtg atggtcaacg gccgtctcac ccttgatcac gagaagtgta
    41821 tggagtgcgg gatgtgcatc gacagctgtc ctttcagcat tatacaggga accgagccgc
    41881 aataccttat caccgtaggc gggaggaggg ggcggcaccc ccaactcggc agagaactaa
    41941 tacgggtatc ttctgaaaag aacgccgtgg cggtggtcaa cagggtggtg gactggattt
    42001 accggaatgc ctacagcgga aaaaccctga ctgaccagat ggatgccatg ggttttaccc
    42061 ggctgcagga aaaaacatgg aaagaaatcg gggaatcatc gtctcccact gtcaaataac
    42121 aaaggaaaag aaataatgct ttttttagga ggatgctcct gatattctcg gattcggtaa
    42181 ctggtaaaag gtttttgaag ttaaagacta atacaacaaa aaaaattatg ttagctatat
    42241 taagttaatt acttcatcct ctttcaaaat atttaactat acaatttaac aaaaacggat
    42301 tgtacatggc attcagtgta catgtcaaca tggaacgatg cactggctgt ggcaactgtg
    42361 tggctgcatg cccggtgaat gcactggaga tttacacgat tgatccggtg acgagcgaga
    42421 agatctattc ggtcagggac gggaagtcga tcagtctcga cctgaaactt gaattatgcg
    42481 cgggttgtgg ggtgtgcgtc gaggcgtgcc catataaagt gatcacattg agcgggcgcg
    42541 gtgcatacac cccgccggta ctcacctgaa tgtgctcacc cgcattcacg cgcatatgca
    42601 attgagaatt ccatgaggtg caaccatggc tgaaaaaatc atcctgaaca tgattaccca
    42661 gcgttctatc gaagaagggc aggcaatgga gaagggaaaa accactcccg aatacttcgc
    42721 ggcctgttcg gtctgcgaaa tgaatcccga agacatgaag aaactcggta tctggaggaa
    42781 caccaacgtc agggtcacga gtgaagtcgg gagcgtcatc gtcaaggcga tcgaaccgac
    42841 ccagtactgc ccgccgggcc ttgcccatat ccggcagggc gtgtgggcca accaggtggt
    42901 cccccccagg acccagtcga ccgggacgcc ccagtatagt ggatttccgg tgacaatcga
    42961 acccgcaatc aacgagagaa tcaaaaccgc cctggaactc gtacagggat ctgtcggact
    43021 ctggaaagga ggaaactgaa tgcctaaatg gataactcag gtcggatgcc cttactgtgg
    43081 gtcatcctgt gacgatatcg aagtgctcgt ctctgacgat gggaagaaga tcctcgagac
    43141 gaggaatgca tgcgtgatag ggaacgagat cttccaccat gtctctagcc ccaacaggcc
    43201 aaagagaccc cgcatgcggc agcccgacgg atctatcaag gaggtctcct acgatgaagc
    43261 gatcgactat actgcaaaaa ccctgctgaa ggcaaagaag cctcttatct acggttttgg
    43321 ttccaccaac tgcgagggca tgagtgcggt cgcacgcgtc gcggagaagg ccggagcagt
    43381 gcttgacaac tgcgcttcca tatgccatgg tccttccttc ctggcaatat tcgataacgg
    43441 ttatcccagc tgcacgctcg gagaggtcaa gaaccgggca gatgtcgtgg tcttctgggg
    43501 gtgcaacccc atgcacgccc accccaggca cacctcgcgt tactcgatat tcccgagggg
    43561 ctttttcacc aacaagggac agatgcagcg gacagtggtt tccattgacc cgagggaaac
    43621 cgacaccgcc aaactcgccg attatcactt catgctcaac caggggcacg actacgatct
    43681 cttcgatgcg ttcaggaccg ttctgcgggg tcatgacatc cctccggaag tagcggggtg
    43741 caagagggag atgataaagg aggtcgcaga gaagatgaag aacgcgcggt tcgtcatgat
    43801 cttcttcggg atgggatgca cccacactga cggtcgcaac cacaacatcg atatcgcgat
    43861 cagcctcacg cgcgatctca atacccacac gaaagcctcg attatggcca tgaggggcca
    43921 ctataatatc gcgggccccg gacaggtctg gtcctggcag tttgggttcc cctattgcat
    43981 tgacctgtca aaggggaccc atgcgcacat gaaccccggc gagaccagca gtatcgacct
    44041 tgcgatgagg gacgagattg actgtttcgt caatgtcggc acggatgcag gagcacactt
    44101 ccccattgcg gcggtaaaac acttgaagaa acacccgttc atcacggtag acccgaatat
    44161 ctgcatggcg agcgagattg cggacctcca catacccgtc cgggtcgtgg gtgtcgaaga
    44221 gccgggtgtc gtctatcgca tggacaatgt gccgattcag ttcaagaagg tggtagacgg
    44281 tccggaaggt gtcccgagcg acgaggagct ctttgacaag gtctatgccc ggatgtgcga
    44341 gctgacgggt tgcgagccga tctgggtcca tgcgaaagaa cacgcgaaag cagcgacctc
    44401 aacggagcac taatatggca gagatcttga tcaaaaacgg gttcgtgatc gatccctcgc
    44461 gcaagatcaa cggcgatgtt gcggatgttg caataaaaga cgataaaatc gtcgataagg
    44521 ttggttcagg cgccagggtg atcgatgcca aaggcaaggt ggtcatggcc ggggcagtcg
    44581 atgtccatgc ccacgtcgcc ggtccaaagg tgaacgtcgg ccgactgttc aggcctgagg
    44641 acaagctctt caggagtcca aacgcccagg agaactaccg gatggagatg ggcttctcgg
    44701 tcccgagcac ttttaagacc gggtacgatt acgccaggat gggctacgcg ttcgtcatgg
    44761 aggctgcaat gccccccctc cactctcccc acgtgcacga ggagatccat gacaccccga
    44821 tcatcgacga agccgcactt cccgtctttg caaacaactg gtttgtcatg gagtacctga
    44881 agaacaacga gatcgagaac agcgcagcct acgtcgcatg gctgattgca gcgacaaagg
    44941 ggtacgggat aaaacttgtc aacccgggcg gaaccgctgc atgggcatgg ggactcaact
    45001 gcctcggcat caacgacccg gtcccgttct atgacatcac accagccgag attatcaccg
    45061 ggctcatgaa gaccaacgag tacctgggac tgccccactc catccacgtc caccagaaca
    45121 gccttggaga gcccggcaac tacacggtca cgcttgattc actcaaactc gctgaaggag
    45181 tcaaagccag gaacaagttc ggccgcgatt cggtgatgca ttcaacgcat atccagttcc
    45241 attcctacgg cggcgattca tgggccaact ttgaatcgaa ggccaaagag gtgatggatt
    45301 atgtcaatgc acagaagaat atcactgtcg acctcgggtg tgtcacgctc gatgaaacga
    45361 ccaccatgac cgcggacgga cccttcgagc accacctgaa caacctgaac cacctgaagt
    45421 gggcaaacgt ggacgtcgag ctcgagactg ccgcgggcgt cgtgccttac gtgtacgaca
    45481 agaatatcaa ggtttgcggc atccagtggg cgatagggct cgaacttggg ctttttgcaa
    45541 aggaccccat gcgcacgttc gtcacgacag accacccgaa cgccggcccc ttcacgaggt
    45601 atccccgcat catcaagtgg ctgatgagca agaaggcgcg ggaggcaacg cttgactcgt
    45661 tcaagtataa ggataaggtg atcgatgcca ccaacatcca cagtatggac agggagctca
    45721 cattatacga gattgcacag atgacgaggg ctggccccgc gaaatccctt ggcctctccc
    45781 acatgtatgg cggtctcgga gcaggccttg atgcaaatgt cgcggtgtat gacctcaact
    45841 taaagaacat gccaagcgat cccgagatga tcgagcgtgc cctgcttcgt gcaagctggt
    45901 tcatcaagtc cgggcagatc gttgtcaagg acggggagat cgccggccac ggtaacaagc
    45961 ggacagtgtg ggtgaatgcg aaagtcaaag agaacccgca ggtcacgagg gacatccacg
    46021 aaaagttcct gaagaactac actgtgggaa ttcagaatta ccctgtctgg gactctcttg
    46081 caccgaaccc gctggtcatc gatgtcgacg tggaggccta aaacatggcg aagataacgc
    46141 tgaaaccgtt caagaccccg gagctcttcc tcgagtgccc catcatctcc cccgatatgt
    46201 ttgccggaaa gacaccagct gagatcgcgg aactcgaagt ccgcgagggg aaaacaacgc
    46261 aaaaactggg agattacttc ggagtcacag gatctgccgg aaaggatgct ggtgagacag
    46321 aaatcctcat agacggcgac tgttcccggg taaaatacat tggatcaaaa atgaccgcag
    46381 gatcaatcac catcaacggc aacgcagaca tgtatattgg cggatggatg aaaggcggaa
    46441 agatccatgt caaagggaat gtcgactcgt tctgcgggat agcaatggaa ggcggcgaca
    46501 tcattgtcga cggcaacgcc cagaattacg tggggtgtgc gtaccgcggt gactggcgcg
    46561 gcatgcgcgg cgggactatc cacatccacg gaaacgccgg caacgacatc ggcacgttca
    46621 tgctcggcgg caccatcatc atcgagaaga atgcgttcat acacgtctca acgcacgccg
    46681 agggagggac tgtcgtgata aagggtgatg tccagggccg cgtgggcggg cagatggtaa
    46741 aaggcgacat ctatgtcctt ggcaatataa agtatatgct cccgggcttc aaggagatcg
    46801 acagggtcaa gaaggagatc gcaggggaga ccgcgacgtt caagcattat atcggagatc
    46861 tgggagaacg ccatccgaag tcaaaaggaa aggaaatcta cgccaacctt ttcgtccggg
    46921 catcttaata aaaaagatct ctttttttcc ttgatgttaa acgtattaac tccgagcgga
    46981 aataaactac tattgatgga ggacatatga cagaccccgg catcgaattt ttggaaaaga
    47041 ccaggtaccc cgatttttcc accgttgatc tcatcatgag ggtccccgag cccccgaacg
    47101 agctgccagt tacggagaat gcacgggtga tatcgctgcc cagtccgaaa aaggtaaaga
    47161 ttacggatgt gccggtcagg gaagcgctcg agaactggaa gcccccggga ttcttctcac
    47221 ggtcatcgat tacgaaaaat gatctctcct tcctgttgtg gtgcatgcag ggtgtccgaa
    47281 aagctgacgg ggattcgccg atgctccgca acgtaccttc aagcggttca agatacccga
    47341 tcgagacctt ctttgtggcg ggtgaagtgg aagggcttga gaccgggctc taccgctacc
    47401 tccccctttc gcataggatc gttgtcgagc ggcttgattc ggatattcct tttgccatgg
    47461 ggactgccag catgaacttc aacctcatca caagggcggc ggttacattt ctcttcgtag
    47521 gcattccata ccgctcgaca tgggcattgg gaaacagggg ataccggagc gtcctcatcg
    47581 aggcaggcca catttgccag gcgctcatca tggcctcatc ctgcatcggg tgtgaggtgc
    47641 agcctattga cctcttccat gaccagatga tgattcaact cgcccgactg gacccaaaga
    47701 cccagtggcc attgctggtg gggacggtag ggagacgcga gcgtgaactc tgacatgcgg
    47761 cggcttgttg aagttgccga atgcgcgggg tatcgggacg cccgttcctc acactcttgc
    47821 agagatcagg cccaaaggaa gggagaagag ataagtcgtg cgtgttcggc atttttttaa
    47881 aaaaatggat ggaaatatct tctttacttc cgaacccctg actgcggagc ggctctcctg
    47941 gctggtcgag ctgctgaaat attacacgac ccgtctcttc cccgagagcc ttcatcacaa
    48001 tccacggaca cccacgcctc ccttcacgtt cttcctcctc ggcgatgcat gcaatatcct
    48061 gataggccgc gaacatcagc gctccctgga gatctttttc aggctgccat gtttccggtg
    48121 tatcttcgat caaggtgacc ttcatgtacg aaggatatcc attgaacctt tcaggataag
    48181 gtacccgggc caggtcatcc ccatcgcacc tggagacaat ctcccgggtc gttcgatatg
    48241 ggattgcctc atgaatgcca tgggaaaaac acctggtccc ccatcaatcg ggtttctcca
    48301 gatgcgttct ccctacatgt tccagtcttc ctcatgcgtg gtcgatctct tccgggcagc
    48361 agccagaacc gggatctccc ccgagttcta cggatacctc gacggggtcc atgccatgca
    48421 ccgcgaccag cggccgccac accatgtgaa tatcggagaa gcactctctg acatctatcg
    48481 tgtcgcgttc acaaaaggcc tttttccccg gtatctcatc tgccaggaga gtgccgcctc
    48541 gcgtggttac tgcacctttt ctggggataa tggaagagtg gtatctgcat ctctcatacc
    48601 ccaggcacgg ataaagagtc tggaccatat cgtatccagg ttttgcatga gccacaggat
    48661 cttctcgcac acctcttttt ttgtggacgt ggtcgtccag aggaaaattc caagcgtcaa
    48721 gttttcagca gaacgaaaaa aaccgtcgct tgtcatcctt gcgtctcaca gtccctacgg
    48781 gactgaattc acaaagggtg caatttccct tgctgtggca tgtgcccacc agaatatccc
    48841 cacaaggata gtcttcatcg aagacggggt ctacactctc acgggctcgg agtccccggc
    48901 ggggatgtgg gcagatatgg atatgcatgc cctgatagag gcaacatccc ggatggatac
    48961 tctcgaatat tatgtgtata ctccttcgtc gcaggcacgg ggaatcgcaa taaatccatc
    49021 catcaaggga gtatgcccgg taaatccgac agaattcagc caggtactat tgacaccccc
    49081 cgcaggcttg gaagtcgatc accagcgagt ccttgtattc tgattggcca gaagaacgcc
    49141 ctgccctgtg attggctggt cctgcataga tcccgctgga ttgcagcagc ggcaaaactt
    49201 ggattttttc tcctcatgca gggggtcggg tttaaaaaaa atgaccgttc agtcgtggac
    49261 gagcggtaac atccctccag tcccgggcgc ggcaatcccg cccttttccc cttcttcggc
    49321 accttttgcg cgaccggggt agaagtccag gacatcatta ctctcctgaa ccagcctgtg
    49381 aatctcttcc aggggtatgg ggcggtaccc taccatctct gcgctcacgt tcacccgctt
    49441 ctcaaagggg ttgaaaaagg ggaaatccgt caggtccttg ttgtggacgt gcccgtggat
    49501 gacccaacca tcaaaaggtc ttaccagttc ctcggggtca tggatgaata ggtagcgggc
    49561 gccgcgatag ttcatgaaga ggcagtgcga catgtaggag taatagaggt catgattccc
    49621 ctcaaggtag aatattcgac cctccaggcg ttggaggtag gactctgagg attcctggct
    49681 ccgaaaagag agatccccga ggaagaggac cacgtcgttc ttcctgacgg tccagttcca
    49741 gttcctgatg agcacccggt ccatctcaga gatcttcccc atgggaaacg gtcttttata
    49801 ccttggaacc gcattttcat ggccgagatg gaggtcgctg atcacataga tccggggtgt
    49861 ttcagaccag tccggccgtg tcatctggta ccccttctgg atccggaatt cccggagggc
    49921 ccgccgttcg tatatgcccc tggtcgattc aggatatgcg agccacgaac gcaggtagag
    49981 atcgtattct gcgacaggag tcctgccctt ccggagaatt atcctgagaa tgtcaaacac
    50041 cagtggtttt ctctgcgtag tgtccgagaa gacccgacaa ccttcgcgat ccctggaatg
    50101 tctctcgccg caaggaaagt catgcctcat ctttgatggc tgcaggagag tggatttgtc
    50161 cattcggttt cgcatatagc cctctggtcg tctttttacc cggaccagtg cctgccacgg
    50221 ttcatgagct gtcaccgtac tctggggcac ccagggtgtg gtctttccca tgcaggagac
    50281 cagtgaccct tcgggggcgc caagaacaaa tgtatccgag agtgccctga taaactcaca
    50341 gagaccgcgg ttgggcctga cagtatatga gagggcggca agatccccgg ccttttccgt
    50401 ggtactgctg agtttaacct ttattggccc tgaatcgaga gaacactttt ttacggccga
    50461 ttttataggg gtggggtcat catggggttc tgacaggata tcggcgagaa tgagagaggg
    50521 gtgctggtcg gggtgacggc agagtccctg gtcccctatc aaccactcca gttcctctcc
    50581 acccctcgaa cgtggaattc caaggcgcag gtcaagcctg tagcatggct ccggcgtatc
    50641 aacccatttc ccctggaatt ccctgttcat gcgcacagaa atgtattaat ttaatagttt
    50701 taatttttat cgaaatcgat attttttaat taacatttgg agatccacgt aggcaccccg
    50761 taccggccct ccgggaagga gaaacagcat gcgtcacagc gttactcccc acaagatcgg
    50821 gcttggcgga ggcgggaggc cgccggaagt cgtggatgcg gggcatctgt gtcctgcaac
    50881 aggaaaacaa ttggttgatc atgtgcaaag agagaatact caacatttaa ggaaaataaa
    50941 atacttatat ctaacaaacc tgcacgttcg ggtctccctt aacagaacat tccgatatta
    51001 tgaagcgacg atacctctcc ctgatacctt tcgaggacgc ccttgaaaag atgagaaaaa
    51061 ctgcgcccag tccccgcaag gttgagaccg tgctcctgga cagcgcagtc ggtcgtgtcc
    51121 tttccgaggc cgtctatgca aaattttcag tgccaggcat caatgtcgca tcaatggacg
    51181 gccttgcggt gcactcttcg gacacccgca atgccgccga tcggtccccg gtcgtcctct
    51241 ctgatgccgt gaggataaat acgggacagc cgctcccccc atcctatgac gccgtgataa
    51301 tgattgaaga cgtctgggaa gaggacggaa aatggttgat ccggaaatct gcggcaccat
    51361 ggcagttcgt caggccagca ggggaagaca taaggaaagg cgacctgatc ctgccccgca
    51421 ggcacctgat ccgggcgata gacatcggtg gcctggcttc gtatggaatc gcatttgtga
    51481 atgtgctctc cgtgaaggtg ggaatcatct ccaccggcgg agaactcgtg cccctgggaa
    51541 tgactccttc tcccgaacag ggggtggagt ccaacaattt ctttgcagaa gcgtacctgt
    51601 cacgaatggg tgccacatgc aagcgatacc cccttgttcc tgatgaccgg gagaagatcg
    51661 cagcgcagct cctcctggca gtcagggaga atgacctggt gctgctctca gccgggtctt
    51721 ctgccggttc gacagactac tccgcagaga tattgaaaga ttcgggaaaa ctgatctttc
    51781 atggagtggg catcaaaccc ggcaaacccg tgatgatggg gattgtcgag aaaactcccg
    51841 tcctgggaat gcccggatac cctgtcgccg cccacaccgt cgtgcgggag tttgctgcac
    51901 gcctcctcga gtactggggc atggcgccac atccgacata cacggtgaaa gcaaggctcg
    51961 gccagaacct ttcctctgac cttggctacg acgaattcgt tccggtatcg acaggaaagg
    52021 tggatgggag atacgtggtc atccctcttt cccgcggttt cggggtgcag agtgcgctcc
    52081 tgaaatcgaa cggatacatt cacatcccat catcagctga agggctggaa gcaaaacagg
    52141 agatcgatgt caccctctat gagaacccgt cattcatgga acgctctctt ttgatggtgg
    52201 gttcatcgga cacgccgctg cagatccttg ctgactgcat ggttgacagg agtatcacgc
    52261 tgaggtgttg ccccaccagc aaccttggcg gcatacttgc actccgcgcc ggtatctgcc
    52321 acgccgcacc ggtgcatatt cccatactca aggaccacac aatccccgat caactcaaag
    52381 cgcagaaagg cctttccatc cgaaggctgg tcattgcaga acaacctttc gggcttgcga
    52441 caagaaatgc agtacgtctc gaagacctca agaaccttca gatcatgaat tcacagcgcg
    52501 ggaccgcaaa ccggttgctg ctcgacgcgg tcctggccag ggatggtatc cctgtttctg
    52561 ctctgaaagg atacgagaac gagctgaaga atcctgatat gatcgccagc aacatctcct
    52621 caatgatcgc ggatgccggc atttgcacac aacgttcggc tgccaacgca ggacttcgat
    52681 ttctgccact cggtatcgat gcgtacgagc ttgtcatgag ggctgatacc atcgaggatc
    52741 cccgtgtaaa agagatgata gagatgatac agtctgatga gttcaaagag agacttgttc
    52801 agaatggggg atttgacaca ggaagcacgg gcaagttccg tatctgattt cctcctgttg
    52861 ttttctgcat gaagataaag gatccaggat cgcactacga gaacccggat tggtgagaaa
    52921 catcgatccc cctactccga tatgccgggt tccccctccg ttcacgtctt atgcgggatg
    52981 acgtaagcga aaaacagccc ggttctgcaa cattttttac cgtacccccg aaaaaagcgc
    53041 tcaattcttc cccgtgcccg taagggggaa ccagcaatgc tgtggattcg accatggaca
    53101 tgttccaaca tcgcatcttt gctggcaata cccctgtgag accttacgga cggccaaatt
    53161 gcacttacct tggctgcacc aaatgatttg atgcaatata caattattcc ttgagaatag
    53221 ataaacatta ttattttatt attgctcttt gtaaacattt ttaatttaat tttttgtact
    53281 cctttccgtc tttctccctc aagggagggg gccaggggtt tttgcaccac ttcggcatga
    53341 aagcacaact cttatcatac ccgatctcaa actcctgcct gctggtgggc gcaagagatg
    53401 actgagaaga agcctccgga gcagcgcata catgccgagg aggaggcgcg gagacggcgt
    53461 gtcagggggt tgataaagct cctgcagagt aaataccttg atgaccgctg gaaggcagca
    53521 gaggccctcg aacgcgtcgg ggatgcgacg gccgtccagc ccctcatcga tgccctggac
    53581 gacccgtttg tggacgtctc ctggatcgcg gcaaagacgc tcgggcagat aggtgacggg
    53641 cgggcattcg agccgcttct ccgggcgctc ggttcgaaag agaagtggct ccggatcggg
    53701 ggcgccatcg gcctcgggcg gctcggcgac cggcgtgctg tcccgctgct gctcgaatca
    53761 ttgaaggacc cgcaggccct tgtccggaag cacgcggcat gggcccttgg catgctgggg
    53821 gatgaatcct ccaggaaagc gctcgaagaa gcacaggtcg atcctgacaa gagagtgagg
    53881 gaagcggcta cggaggccct cgccaggctt gatgcatctt catgagttcc aacccgggcc
    53941 gcacccgcgc gagcttgggt attctttcac cctgcacgcg gtgcattttt cccgggctgg
    54001 ctgcattttt gcagtatgat cgaatggatc atcctcctgc tcatcgcagt ggccaccctg
    54061 attgcatgga gatacttcgc gctgcgcaca acccttgaac agagagccgc tgccctcttt
    54121 gaggcgtgga gggcagagag gatggagacc gaggtgaagg caagggcaga cctgctgcac
    54181 agggagtgga cgctccagga agaggaccgt gtccggcgcg acgccatcgg caggagcggg
    54241 gccgtcataa ggggaaaagt cacagaacac ctgaccccct acttcccgga gttcccctac
    54301 gatccacgcg atgccaggtt cctcgggaca cctgtcgatc tccttgtctt cgagggtctt
    54361 gcggcaggat cactcgagag gatcaccttc atcgaggtaa agaccggaaa gaccggtgcg
    54421 ctctcgaacc gggagaagca ggtcagggac tgcgtgcaga agggactggt ccgatacgag
    54481 gtgctccacc tcaggggaga tgaaaaagac tcctgacctt ttacttctct gcctgaactt
    54541 cgtttccagg aggctgagca tccatgtcga ttgtcccatc cacgtacgtg gtcagctggg
    54601 taaggagaag cgggtcgagc cccttctcca ccgcgagaaa tatccccgag gcgtcaacca
    54661 gcctgagctt gctggtgata aagtggatga acttcgagat gtttgtcgaa gggatgtaga
    54721 tgaggagggt actgatagaa tcaaagagca ggcaggcttt ttcacccgac agagtgttta
    54781 aaacctccgt gatggcgatc ccaaggtcag tcaggttgga tgggctgttg atgaacctgc
    54841 agtcctcctg tccagggggg accttcccca tcgcgtactt cgtgatggtg tcgatgaagg
    54901 atacccgcgc catctcgatc ccctctgcct catacagcct ttttaatacc gctgacgggt
    54961 ggttggatgt gatcaccacc acccggtacc cctgccccgt cagagtgcgg atgatagcaa
    55021 tattattcac tttcagtctc tcggccgaag agaggactaa aaagagccga tcttccccga
    55081 gtcgcgaaaa gtcgatcttc atcgtggcct cgccgttcat ctagtcagga agtcgcgata
    55141 tgcaacggga atctcttcgc gctcctcaac tatcgcctgg ttcaaatccc tgaagttggc
    55201 catgatctgc tctgcgtgcg agatctgaac cgcgaggtcc atcttcactg aatcgatgtc
    55261 gggcttatcc ccctggagct gcaggaggag tgcggccatg ttgtcatgga ggatctggat
    55321 gggattcttc atccgtagcg ccacctccgc gagatatccc accagaacgt agtgcttctc
    55381 ctcgattgca ctcttctgta ccaggatctc ggccttcgca cgatccctct cctcagatat
    55441 ctgtgcaacg atccaggtta cgataagaaa catcgccgcg cgtagcaggg tcgcccatgt
    55501 gaagtccccc acgaagaggc ttgtcgcgag agtggctgca acgagtgcac ctgccacgta
    55561 gagcgctttt tggtaatacc ataccgctgc gaggacgagc agcacgtaaa agatatgggt
    55621 gtaaccgatc gcggtacgaa ggaataggtg cacgccgatc tcaagggcga tgcagatcat
    55681 cccgaggaga accaggatcc agagacggtg agtagtattt gcaggtccat catcgcactt
    55741 tacgcgcgat cccgcccctt ctccccttcc ttcagggttc acactctttt cattctcgtc
    55801 gcttgcatat taagattggc agaaaaggaa cccggtatgc ccgttcatac cggtgactgg
    55861 agggaggcga tgtcctcccc cgaggcaacg aggatctccc tttcacccct gtaggtctgc
    55921 acagtgccgt atagcgagac ctcctgcccg ggccggggcc agccattctg cacgtgctcg
    55981 gatggtatga agaccttcac acccgacaca accagtacct ggtggccacc gctcgcggtt
    56041 atcgtactct cctcaaccat tccctggtga tggaccagtg atccctccgc actgtcaggg
    56101 gcgtatggaa gggagaaggc gcccttgcca accgattcca ggacaaatgc agagaccagc
    56161 acggccagga cgacaagtac caggatgagg accgcaacct tctcctgacg gacaagtatc
    56221 acggaaaaga gatatcacct cgccctttat aagcaccgac agtttgatat gttaacggcg
    56281 ttaacatgtg tgtcatgcca gagatgaact tacctccatc atctctcacc gtccttcaca
    56341 tcctcgatga aggcggggca atgacccata aggacctggt caagaagaca caacttgcac
    56401 cacgaacggt gagatatgca ctgaagaaac tgaaagagca gcacctgatc atcgagaagt
    56461 tcaacttcaa ggacgcgcgg cagatcatct accagaaccg tcccgttgcg gtgacggcat
    56521 gaaagggaga gcctgcgaca tcatggtctg tgatgccatg gtccgcaggt atcttcctgc
    56581 aatgcgggcc gagatggtat tccgccttgt ccacgagcgg ggactcagcc agagcgatgc
    56641 cgcaaaaagg ctcggcatca cccgggctgc agtatcccag tacctgagca ggaaacgcgg
    56701 tgatgccact ttcgaactct cgcctgacat gaatgccctt atcgaccgct gggtcatggc
    56761 ggtctccggg agggatgacg gtatcacgct ctgcgacata tgccgctgcg ccatgaaaga
    56821 ccccgtgcag caggagaccg gtgtgttcat acgtggtcgc gagggcgatg ccggtgcctt
    56881 ccccccctcc tcctccagta ccagaagaga agaagggcag cgataaccac gcaggcaact
    56941 gcgatcttga tgagatcggg gcctttttcc cccttttcgc tccccgcctt ctcccagtcc
    57001 ctctcgaatg caagtgagta atactccccg actcccgggt gatggatgat cactcctgct
    57061 tccctgttga aggtcgggga gttcatgttc cagttgatgc tgctgacaag aaccttctcc
    57121 ctgtcgatga tcacaccctt gttgtggatc ttctcgacgc tgtcaggccc gagatatgca
    57181 cagcgggctt cgagcggcag gtgttcggat gccgccatgg cgttgagcag cccgaccatc
    57241 tcgtcgttgt cggctttccc gtcgatgttg taatagtagg agtcgagaat aatcctcacc
    57301 gggatgccct ggcgggagac atcgaccgca tcagcaagga aaggattcag gcagagcgtc
    57361 gtctcgttgg tgatgtacgc catctcgatg tccacgctcc gccgggcacc ctggatgagt
    57421 gagtgcagca acccgctcgt gtccggcgat atgacaggaa caacggttgc cccttcaaac
    57481 acatagggag aaaattcggc cgtataggtg cgggaagagg cgatctccgg atcaccctgg
    57541 cgcgggacgg cgtaaacagc ccattctccc cccgcatcgt atgtatacac cgacctgaaa
    57601 tattccgcaa ggcccgggtg cgtgactgcc agtccccatc cccggttgcc tctcctgcca
    57661 cgttcgggga agccgttctc cttgaagttc tcgctcgtaa ccatcacgtg gttcgagtct
    57721 atcaccatgt acttggagtg gaggaaacgg taagggctgt gcaccagcgc ggtcccctgc
    57781 atggccctga cagggatgcc gcacccgagt agttgagcca tcaccgcgtg ctcctcttcg
    57841 ggtatgcctc ctaccggccc cccttcaatg agcactttga cgtttactcc ccttgcatgc
    57901 gcctcttcga gagagccggc aagggccggg ctcgtgaact catagatgct caccaggatc
    57961 tcctcttttg ctccccccac catggccagg aagacctccc tcgagcagtc cggtgaaacg
    58021 aacgcgacac cgcttgcatt gggaaacatc gccggttcga accgcgactg cccgagcatc
    58081 agcacccttt catcccacat gccttcggaa aaggagtgca cctgcccctg gcgcggcgag
    58141 actgtccccg gccaatccac cttctggagg cataccctcc ccgaaaatac ctcgatctcg
    58201 tcggcagtgt tggagagctg gaaccgcccc gtccgtatca tgtccggcac caccggagaa
    58261 ctcccgtccc attcgaaatc cggatactcc ccgtggctgt tacggtaagc ctctgcgtcc
    58321 atggccacca ccgtcaggcc tcgaagtgtg gtcccgggag gaaaacggat ccctccctcg
    58381 ccatctgtca ccaggatgcc gtcggcctgc ccccaccctt cgaggcagag gtactcgtca
    58441 cggtcatccg gcaggtatgg atcagggcag aattccacaa gctcaatgcc tgtcaccggg
    58501 gctgcgagga ccagcagcag catgatcgtg gtccggatca cgccagaaag gtgggaaggc
    58561 agggaggtat taacctgctg aaatgcatct ctcttttagg aatgacaccg gttcgccccc
    58621 cactggtgct gaagatcggc gggagcctcc aggactgcgc cgggtcgata tgcgcccttc
    58681 ttgtggacct ggacgttccc gtccttgtca ttcccggcgg gggagcattc gcagacctgg
    58741 tgagagagtg ccggcttgaa ggaaccgcag cccactggat ggccgtctca gcgatggagc
    58801 agtacgggtg gtacctctct tcctttggcc ttccagtcac caccaggctt gatatcccgg
    58861 gcgggcccgc gatcctgctc ccttatggcc tgatcagggc aaaagacccc ctcccccacc
    58921 gctgggaggt gacctcggat accattgcag cgtggtgtgc ccacaccctg caccttcccc
    58981 tccttctgct caaatcagtg gacggtatcc gcgtggaagg acgcctctgc ccccgcatcc
    59041 ggtcccccgt agaaacggat gatgtcgact cctgcttcct tcctttcgtg ttgcgccgcc
    59101 ggatgcatgc actggtcatc tccggcaggc accccgaccg gttacgcgct gcactcacgg
    59161 gacttcccac catcggtaca accatcggct tttaagtgtt tcaggagaga taatatgaga
    59221 acattctatc aggagggttc catgccgata agaaagtgca catcatgcaa ttcacccctg
    59281 gccgaagagg gcgccgccga attcgggtgc ccgaagtgcg attatcagat caggagatgc
    59341 taccggtgcc gcgagcagag catcccctac acgtgcccga aatgcggatt cggggggccc
    59401 tgatccatgg gcacggtggc tattatcctc aaggtaatgc ccgagtcccc ggaagtggac
    59461 cttgcggtcc tgaagaggga gatacaggag aaggttccat ccgttcggga tatccgcgaa
    59521 gagcccattg ggtttggctt aaaagcgctc aaggtggcag caatcgtgaa cgatgccggc
    59581 ggggagagcg acgcggtgga agccgagatc cagaagatct ccggtgtcga gcgcgctgag
    59641 attgtagaac tgacccttac ctgaaggagt cccgcctcca cccatgcatt cgcgtgggag
    59701 agggaggaat actttttatt cccgaagttt ttgcagtatt tcgtgcgtga cggcacgtat
    59761 tttatccacc gataccccaa actgcgccac ctcgtcctcg gcaatcttga taggcacggg
    59821 aaagacgccg ctgcggttga tccttgcagg gacaccgatg cacacatccc ctatgtcgtg
    59881 cacctcgctc cggatatatg cagacaccgt gagtatgcgg tcctcgttcc caagcaccgt
    59941 ccgcaccagg gtggcaatcg cttcgcctgg cccgtatacc gttgctccct tgttccgtat
    60001 gatctgctcg ccgctcgatt tgacagtcgc taccatctct tcaacgggaa gccgcgagaa
    60061 tgccggaagg ttgctgatct ggatccctcc gatcgtggtg gcagaccaga gaggtaccat
    60121 tgaatcccca tgctcgccga tgatacgggt gtgcacctcg cttacgtgga cgtggaagtg
    60181 cttggcgatg agtgatttca ggcgcatgga atcaaggtgg gtcccgagtc cgaagacctg
    60241 gtttggctgc attcccgagt atctcaaggc taccgatgtc attacgtcga ctgggtttgt
    60301 gaccatcagg atcatggcgt gcggtgcaca ttttccaata accctggccg tatcggccac
    60361 tatccgggcg ttctggaggg caaggtcaag gcggtcctgc cccggcctcc ttgggactcc
    60421 tgccgtgcat accactatgt cggaatccct ggcatccatg atgtccgttg tccaggagag
    60481 gtccactgcc tggcctgatg cggcaaaaga atctataagg tcctgggtga tcccgcagag
    60541 caggtcctcg cgcccggccc tcccgtggag gaggatctcg tgcacatagg gaatgcctgc
    60601 aagggtatgg gcagaaaaag tgccaacgtt tccggtggcc ccaacaatgg tgacttttga
    60661 cataaggcta ccgccaaggc gaaatgggga cacgttctcg gtcgacggct cgcggctgcc
    60721 tcttccaggc gcattcctcc tccgccccgc gaccccatgg gcgccgaatg ttcacggtaa
    60781 gtctgctccc ttccgggcct gaaccg
//
LOCUS       DAOQYR010000002        56193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_598_length_56193_cov_8.775945, whole genome shotgun sequence.
ACCESSION   DAOQYR010000002 DAOQYR010000000
VERSION     DAOQYR010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 56193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56193
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_598_length_56193_cov_8.775945"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="PKM87_00320"
     CDS             <1..933
                     /locus_tag="PKM87_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ28540.1"
                     /translation="THLFGPVSSRRLGRSLGVDLVPYKVCSFDCVYCEVGSTTMRTVT
                     RQDFFPPSEVEEELVRFFSGEPSLDFITLSGSGEPTLSLSLGRVVRFVKSRFPAYPLA
                     VLTNGSLLWDSDVQHEILPADVVLPTLCTVDEEIFQKIHRPAPGLTIKKIVEGLRDFR
                     KIYPGEIWLEVFLIPGINMDRDHLSALGDEIRRIHPDRVQLNTLDRPGTEAWVRPASR
                     EQLADAAYHLGLPGAESIEPVPYQRSQEQISADPASMIVEMISRRPCTVEDIALTTGL
                     HLQEVGKLLRALSRDPRLMTKREERGIFYSWVGD"
     gene            complement(912..2018)
                     /gene="cofH"
                     /locus_tag="PKM87_00325"
     CDS             complement(912..2018)
                     /gene="cofH"
                     /locus_tag="PKM87_00325"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HNQ28541.1"
                     /translation="MDLRTLLSDARAGHRLDREEALFLLNVKGREVFQVCSAADQARE
                     EMVGPVITYVRNQNLHLSNICKNLCGFCAFGRSATDEGAFCDDRDTVGKKVRLALDRK
                     VSEICLLSGVNPEFDAARYLEILSWVKEVAPEIHVHAFSPDEVSHAATRSGLSTREVL
                     LAMKAAGLGSLQGTAAEILVDRVREIICPAKVPTDEWVRIIREAHGLGIPTTATIMYG
                     SCETPRDRIAHLSILRDIQDGTGGFSELVPLSYLHQNTRLYVEGKAPPGATGREDLLL
                     IAVSRLFLDNFKNIQVSWGKLGVKLGQVALLAGANDLAGTMFSDDVSGDAGASGSDFL
                     DPREMERMVADIGRVLRRRLTDYRLADDQSPTQE"
     gene            complement(2037..2900)
                     /locus_tag="PKM87_00330"
     CDS             complement(2037..2900)
                     /locus_tag="PKM87_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HNQ28542.1"
                     /translation="MSRNDPEENKPGLWEQFRKSENPWVSLARELAWVAAVVGGIALA
                     LFLVSGTWPAVVTIESESMVPHMQVGDLVFVVSADRFGGLESWTSGIEKGYLKYGDYG
                     DILIYRPNDAPNPPVYIPFLTKGVHPIIHRAMDRIDAGENIPKYYNLYRGQATPARYL
                     PATIQNNSLVLEDGTVVTPQNADPMNGYLVETTLVSPHAGYITKGDNNQVSDQGGYLS
                     SVSGEVIMPVKDEWIVGKALLSIPLLGYLPLNIVPVAIILIALMLVWEYYARKKGERS
                     EKRDKLAKGKR"
     gene            3065..4462
                     /locus_tag="PKM87_00335"
     CDS             3065..4462
                     /locus_tag="PKM87_00335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II small subunit"
                     /protein_id="HNQ28543.1"
                     /translation="MLDAATIVQRFLATKMQVHPEVVRYLSEKDDPGLIDQIIASVPG
                     DTIVVSLQHIPGLHQDRDGARFLADPSCEVLRGAAGTTKSISHITDYCHYFRDRYQRL
                     GEYIRPRAAPMPIEALKKGARYRQEACSIMGLVMESKATTNGHRIVEIEDSSASISVL
                     FHKERPQFGEAETLVPDEVVAVRGTLSGDGRLFFAEQLFRPDIPLTHAPFRSQDPGSA
                     ILISDVHVGSNTFLDEAWNRFADWLSGSDASYLLIAGDLVDGIGVYPNQEQELTITNI
                     YEQYEVFGSMLRDLPSRMKIVICPGNHDVVRAAEPQPAIPSEFTANFPSNCIMVENPA
                     LVSLQGVRVQMYHGRSIDDFISLIPGASYENAAPMMMGMLQRRHLAPTYGKRTPIAAG
                     KEDALIIDPIPEVLHTGHVHIMGIGDYRGVLCVNSGTWQGQTAFQKQMNITPTPGRAV
                     VLDLQTLNTRVLDFT"
     gene            4688..6718
                     /locus_tag="PKM87_00340"
     CDS             4688..6718
                     /locus_tag="PKM87_00340"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNQ28544.1"
                     /translation="MDMFFIVPVCALLGLVFAGYSFMVMKREGTGTELMQKIAGAIRL
                     GAMVYLKSQYKAIAVFMVVLAIVLYLAPGIHPLTSVAFLVGGILSATAGFVGMTAATM
                     ANVRTTNAAREGMAKAFRVSYASGTVMGLTVVGLGLMGLSLLFLTLNALTTLGLSEII
                     NILSGFSLGASSIALFARVGGGIFTKAADVGADLVGKVEVGIPEDDPRNPAVIADNVG
                     DNVGDIAGMGADLYESYVGSIVATMLMGVSLAAATQFVGVASLNLVLLPMFIAGLGII
                     ASIIGSFFVRSNKNESSAIHKAFNAGTFASLILTVIAAYFLVTMVLGPQYIGVFVATI
                     AGLVAGFLIGLITEYYTSYERPPTRKIANSCETGAATNIITGLAVGFESTVFPVVIVV
                     VAILIAAQQAGLYGVAIAGVGMLATLGITLSVDAYGPVADNAGGIAEMSHQPKEVRGI
                     TDTLDAVGNTTAAIGKGFAIGGAALTALGLFAAYSQAVSLEIVNMLEPVVFVGVLIGA
                     MLPFLFCSFAMTAVGKAAGYIVQEVRRQFKEIPGLMEGKAEPDYSACITISTNAALKQ
                     MIAPGILAIAAPLVIGLLLGTHALAGLLVGSISTGFIVAVMMANTGGSWDNAKKYIEQ
                     GHMGGKGSPAHKAAVTGDTVGDPFKDTAGPALNILLKLMSIVAVVFAPMFLI"
     gene            complement(6907..7500)
                     /gene="pdxT"
                     /locus_tag="PKM87_00345"
     CDS             complement(6907..7500)
                     /gene="pdxT"
                     /locus_tag="PKM87_00345"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HNQ28545.1"
                     /translation="MGVRIGVLALQGDVSEHVRGFMAALSQIGAKGGEVVPVRQPADL
                     AGLSGLAIPGGESTTISRLIQKNGLYEPLIRFEGGIFATCAGMVLMAGRVDDPRVKTL
                     GLLDMTVLRNAFGRQKESFEAEIPIKGLDTPYHAFFIRAPVATGTGNGVEVLARIDAG
                     IVAVRKGPHMALSFHPELGNDIRLHRMFLAGLGIGKP"
     gene            complement(7490..8386)
                     /gene="pdxS"
                     /locus_tag="PKM87_00350"
     CDS             complement(7490..8386)
                     /gene="pdxS"
                     /locus_tag="PKM87_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNQ28546.1"
                     /translation="MKLEELRFGTELLKRGFASMQKGGVIMDVVNAEQAEIAEEAGAV
                     AVMALERVPADIRKAGGVARMADPAIIMEIMDAVSIPVMGKVRIGHFVEAQVLESLGV
                     DMIDESEVLTPADEQFHIDKTSFTVPFVCGARDLGEAMRRIHEGAAMIRTKGEAGTGN
                     VVEAVRHMRNIMGEIRALQGKNSQEIVARARELEAPAELLLETVKMGRLPVVNFSAGG
                     IATPSDAALMMQMGADGVFVGSGIFHSSDPRRMARAIVEAVNHFDEPGVIAEVSKGLG
                     QAMKGIDVHTLREDEVLQYRGR"
     gene            complement(8391..9311)
                     /gene="cbiB"
                     /locus_tag="PKM87_00355"
     CDS             complement(8391..9311)
                     /gene="cbiB"
                     /locus_tag="PKM87_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HNQ28547.1"
                     /translation="MFWIRPLVLPLALLFDRLAGDPHSRLHPVALLGTFIAWWGRPDL
                     YPPCLQRAAGAALWAFTVLLFSLPFILAEIFLPWLVLIVAGPVLLKSCLAWRSLEEHA
                     LSVENALSQGLDEGRSQVQFLVSRDPSVLSREQVHSAAYESVSENLVDSIVSPLFYYA
                     LLGLPGAAAFRAANTMDAMLGYRDHRERLGWFSARADDVLNYIPARLTAGILLCYFAC
                     RGRFAPALDCVRKDGRKRPGFNGGLPMAVMAGGTGIRFEKPGVYSIGPGDRTIEDAGP
                     EILSAVRACTILVTLLLMVLMFLALLIPLI"
     gene            complement(9545..9727)
                     /locus_tag="PKM87_00360"
     CDS             complement(9545..9727)
                     /locus_tag="PKM87_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methytransferase partner Trm112"
                     /protein_id="HNQ28548.1"
                     /translation="MKRSLLPILCCPVCKGDLELRVKEENDVEILEGGLYCAACRVEY
                     PIEEGIPDLLPRTHEE"
     gene            complement(9724..10734)
                     /locus_tag="PKM87_00365"
     CDS             complement(9724..10734)
                     /locus_tag="PKM87_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNQ28549.1"
                     /translation="MTCTIIVGGFFGDEGKGKIVAHIAHQDHPTIISRGGVGPNAGHT
                     VKMGEKEYGVRMVPSGFVYPNARLMIGTGVLVDPRVLAKEVEVLGVGDRVFVDGRCTV
                     IDEAHIQRDKQSDHLAKTIGSTGSGCGPANADRVMRVARQAKDLPELARYITDVPREI
                     NTALDKGENVILEGTQGFGISLYYGTYPYVTSKDTSASQIAADNGVGPTRIDDVVVVF
                     KAYPTRVGEGPFSTEMTRDVSDRMGIQEFGTVTHRQRRIGQWDGAMARYSAMVNGCTQ
                     AAITGIDRVDKACFGLTNYDKLTKKALEFLEHAEKDIGCPITLISTGPDVSNIIDIRG
                     ER"
     gene            complement(11043..12203)
                     /locus_tag="PKM87_00370"
     CDS             complement(11043..12203)
                     /locus_tag="PKM87_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNQ28550.1"
                     /translation="MRDYISKKGREIPPSGIRKFFDLALTMEEVISLGVGEPDFSTPW
                     NICEASIYSIEQGGTSYTSNRGLQTLREALSRYLHAHYGLSYHPDNEMIITTGVSEGL
                     DIAIRAVVDPGDEVLVAEPCYVSYAPCVTLAGGMPIAVPCVEKDRFRLNPDRLMERVT
                     PRSKALIINFPNNPTGAVMRREDLQAVADIVCDHDLVLISDEVYAELTYEGSHVASAT
                     VGDLASRTITLNGFSKAYAMTGWRIGYLCAPREICDAALKIHQYVMLCAPVMGQVAAL
                     EGIRRGEEEKDRMVREYRVRRNLFVESLNRIGLTCHLPEGAFYAFPSVEGTGLSDYEF
                     AERLLKEEHVAVVPGSVFGQGGAGHLRCAYAVSRKDLNEAVRRMEQFISQVK"
     gene            complement(12212..12703)
                     /locus_tag="PKM87_00375"
     CDS             complement(12212..12703)
                     /locus_tag="PKM87_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNQ28551.1"
                     /translation="MDEKDLLLLQLLEENSRLTTREMAVMAEIAPEEIEARIAALEGA
                     GVIRSYSAVIDWERAGNGEVSAIIELKVNPERDFGYDRIAERIARFRQVRSLRLITGA
                     YDLQLTVTGKNMQEVARFVSEYIAPMDRIRETATHIIMKSYKENGRTFCEKEGSERIP
                     YSL"
     gene            complement(12711..13994)
                     /locus_tag="PKM87_00380"
     CDS             complement(12711..13994)
                     /locus_tag="PKM87_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORC1-type DNA replication protein"
                     /protein_id="HNQ28552.1"
                     /translation="MAEDQSASVGLFKKYLESNKIFKNREVLRHSYRPQILPHRRPQI
                     DQVASILAPSLRNETPSNILIYGKTGTGKTASVRYVGSELENAGIHMGTICRVVHLNC
                     EVIDTQYRVLAQISKTLLGEDETASDKARIHIPMTGWPTDQVYQELKNQLEAMGGVLI
                     IVLDEIDKLVKKSGDETLYNLSRINSDLKKSKVSMIGISNDLSFKDFLDPRVLSSLSE
                     EELVFPPYNALQLCDILQQRAEMAFLDGVLDEGVIPLCAALAAQEHGDARRALDLLRV
                     SGEIADRDESDRVSERHVKGAQAKIEADSMIECIATLPTQSKVVLYAMLLLDQMGQTI
                     FTSGEVSRIYKEIAPAMELDVLTHRRITDLISELNMLGVINTRVVSRGRYGRTKEMWF
                     DTNIHKIWDVITADPRLSTQGLAERDVQWCRSLFR"
     gene            14715..15209
                     /locus_tag="PKM87_00385"
     CDS             14715..15209
                     /locus_tag="PKM87_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HNQ28553.1"
                     /translation="MKSDHERPVAIKAAVLTVSSTRIEENDTSGKEIMTLLGKAGIKV
                     GNYAIIPDNIEAIQREVLDSIKSCNCVILNGGTGLTHDDCTIEAVSPLLEKKIDGFGE
                     LFRAKSVNEVGTAAMLSRAIAGVCHEKVVFCIPGSTPAATLATRELIIPELRHIISHA
                     MKRS"
     gene            complement(15265..15774)
                     /gene="cgi121"
                     /locus_tag="PKM87_00390"
     CDS             complement(15265..15774)
                     /gene="cgi121"
                     /locus_tag="PKM87_00390"
                     /inference="COORDINATES: protein motif:HMM:NF011465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Cgi121"
                     /protein_id="HNQ28554.1"
                     /translation="MRCGIYQVRMCIHDLEGFLKRIQDISRRNDAHVILFNAMNMAGK
                     AHTESALHHAFRALDQGSMISSRVEVESLLYAAGSRQIVHGTRFGVQKGENAAYLCIC
                     PDNASARRELMKFCSPADHEDWDSITPAKAAHLCELFSITPQEAEVVGPGRIQELVLE
                     RVALLEVYR"
     gene            complement(15774..17930)
                     /locus_tag="PKM87_00395"
     CDS             complement(15774..17930)
                     /locus_tag="PKM87_00395"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNQ28555.1"
                     /translation="MKVAALPLPHYLKERYRSLGIEDLYPPQAECVEKGLFEMTNLLV
                     TVPTASGKTLIAEMAMHSHAKREGKSLYIVPLKALASEKYEEFGGKGVRVGISTGDFD
                     RRDEYLGRNDIIVATSEKVDSLIRNRAPWLSEITLLVVDEVHLLDSPDRGPTLEMVIT
                     KMRAMNPRLQIIGLSATIGNPRVLAGWLDAALVTSTWRPVDLRQGVYMNGVIQFDGQS
                     RAVVSVSKYEDINLLLDTVAEGGQCLFFVSSRKNAEAFAKRAAKALMCEDPELDTYRD
                     RLKKIDATGTGESLASCVRMGAAFHHAGLPRDARAIVEEGFRKGLVRCISSTPTLAAG
                     LNLPARRVVIRDYRRYETGVGMVPIPAREYHQMAGRAGRPRLDPYGEAVLIAKDEEER
                     ASLFEFYIHAAAEDVNSQCANESALTTHILSLVASGFARSDTEIQEFMARTFYTHQHK
                     GSRLIGSVTERALRFLDTAEMVVENGGRYSATEYGGLVSQLYIDPRGAESIVRCMCAA
                     KDYSDMGLLQTLCSTPDMYTLYVNNRDLGYLERFLLEHEEELWQPLGFGEEEEYFRAL
                     KTAMLLRDWSMEVPEATICERYSVGPGDIFNMVESVNWLLHASDRLAGMFAPRFARDV
                     REYGVCMKHGVKRELLPLVRLKHIGRVRARSLFHNGITTPEELKKAESGKVAAILGRG
                     VAAQVMRELGEDEDTNEKPSAAVGQATLSSFGEDGQ"
     gene            complement(18079..18618)
                     /locus_tag="PKM87_00400"
     CDS             complement(18079..18618)
                     /locus_tag="PKM87_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNQ28556.1"
                     /translation="MMQELKVAGNRIGVLIGKGGLIKKELEEKTGTVIAIDSQEGIVR
                     VEGEDAVPFLRAVEVIQAVNRGFSPDRAFRLLGDEDLLLEVIDLSGLADNPRQMDRLR
                     GRIIGRDGRAREQIEHMTHTQISVMGKTIAIIGLPEQMKVARAAIDMLLEGSPHEAVF
                     SYLEKKRQDAKQDMLEYYY"
     gene            complement(18615..19427)
                     /locus_tag="PKM87_00405"
     CDS             complement(18615..19427)
                     /locus_tag="PKM87_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase RIO"
                     /protein_id="HNQ28557.1"
                     /translation="MSIDRIEDRFDRKIAELGIRIKDMDQLKVRDDVFDEITLLSLYR
                     LVHKKWITAIGGSISTGKEANVFFGERENRDLAIKIYRIRTANFNAMSEYIMGDRRFS
                     GIRRTRKDIVFTWTRKEFANLSRAHETGLPVPEPLVWDRNILIMEFLGEEERPYPQLR
                     SVHLDDPGEIYRETIGFIKDLYQKAGLVHADLSEYNILYGDRMYVIDMGQAVTVDHPR
                     ALHFLVRDIANINRFFGTKCQVRVEREIFSEVTGIAQPEQITTIKEDEGRDA"
     gene            complement(19442..20383)
                     /locus_tag="PKM87_00410"
     CDS             complement(19442..20383)
                     /locus_tag="PKM87_00410"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNQ28558.1"
                     /translation="MDAYALATRNTVEVVTDEELREVLARPKKRVYAGYEPSGEIHLG
                     HLVTVNKLVDLKHAGFDVVVLLADLHAFLNRKGNMEQVRELAMYNRRCFEGLGLTDVE
                     YVLGSDLQLDRDYELMVLRLSQQITLNRATRSMDEVGRQMETPTVSQMIYPIMQMVDI
                     ARLGVDAAVGGIDQRKIHMLAREHLPSVGYPSPVCIHTPILNGLDGKKMSSSSGNYIS
                     VADSVEEIQKKCQKAFCPPECEENPVLQILQYHVFPRVSEVVVRRPEKFGGDHTFQSF
                     SEIEEAYRKGEIHPLDLKKTTATYLSDILACVRDHIG"
     gene            complement(20708..21730)
                     /locus_tag="PKM87_00415"
     CDS             complement(20708..21730)
                     /locus_tag="PKM87_00415"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HNQ28559.1"
                     /translation="MIRVAINGYGTIGKRVADAVAAQPDMKVVGVSKTKPSHEAFVAK
                     SRGYPLYIADLSKKAAFEKAGLPVAGSVDDMLKAADIVVDATPGGVGEKNRPLYEKVG
                     IKAIWQGGEDHEVAGFSFNSDCNYRDAIGKQFARVVSCNTTGLCRIINAMDKAYGVEK
                     VRAVMVRRGGDPADIKRGPIDAVVLNPVHIPSHHGPDVQSVLPHINIVTLAMIVPVTM
                     MHMHVVQMDLKKEASREKVLEIIEKHPRMGLVRKSTGITSTAELKEYAMDLGRCRSDL
                     WENGIFEESVSCIGKELYLFQAIHQEADVVVENIDCIRAMMGTEKDPAKSIATTNKAM
                     NFVAIN"
     gene            22101..27008
                     /locus_tag="PKM87_00420"
     CDS             22101..27008
                     /locus_tag="PKM87_00420"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HNQ28560.1"
                     /translation="MTIVKVGSKKELCFSCILISVILFGMVGCVSGETYYINSTVLNA
                     ENPVFIIEEPGIYLFDEDISGIPTSQDEYRGAIWIKTSDVVLDGQYHSLSSDLTDILW
                     TNSALIISSSEDFGDTPDQGSDLENVTIRNIYFDMWQNSVFAYNTNSLILTGNVFSNT
                     QSAIYCGNSQELKIEDNTLNPGCNPYDYISITATRPSSGVLIANNSIYGGGPTSSINI
                     DSEYVQDVIIHSNILQGGSESDHPGIFSLADKVIIENNLLQCERSCVFISGDEYLIRN
                     NTFESNEQFIQFNGRNSIIEDNQFGSSPSYGCGVNIGDEYNYAENVSFSKNKVDSEFG
                     GNLYGNRCKIIDCEFSGWDGIAITGNENIINNSIFYSTGHTMVIWLTGDNCEIINNSL
                     YGGSPGQGGSIIQIYGSNNILSGNTIQGEDYGITMIDGMANFICNNTISGSLNGISIS
                     DSDMNQIYGNFITENEEAGILITVINTDCQNLIFNNYLKNLNNIVFSANSPSCSWNIE
                     SGMGPNIIGGPRIGGNYWGAPDGTGVSDLALDEDNDGFADEPYEIDAQNIDYYPLVHF
                     TSPPEYGIYYLNSTVLNNETTRYIIDQPGTYLFDEDIIDLEFPCDTSGTFSEGYPLIW
                     VRSSDVVIEGNGYALDAIDQSNGIPVSAIFIGDSGDEIESLRLENVTVRNLSLSNWYI
                     GIESHSVANHIIEKNFIKNCGFGILINGELPLFCDIRVRMNTITSDGFEDNSGYCGIQ
                     INMGDFDENIVAIVENNQVSLLEGCAIRVDGGTAQINSNIVTNCGEGISSGSAGIISN
                     NIVTGSNDWAISGFGYEMEIINNTVSQNPGGGFYLGPAEYSVIRDNIIEGNGLFGIIF
                     DGADCHIFNNYLNNTQNLDLEALGEWCPQHSWNASVPIEEQNIAGGPSIGGNYWGTPE
                     GSGYSQTAVDQNHDGFADEPYVIISGYWDMDYDGTPEYHETIDYFPLVTFDQIPDNQP
                     PYAFLSFDFPDSSNKDIPTPDDVITFFDNSWDPDDGDTITEWSWDFGDGETSDEQNPL
                     HQYSQSGFYTVNLKVTDGNGAMGSCEEEILVNYPPVVDFTMDIEPPLIAGQIVHFTGT
                     ATDQDGTIIELTWQVFSGSQPYPEIVSTDINMWYEFTTPDLYVITLTAIDNLGSPGIA
                     GYILVVNELEETLYFLMAPGVEFDGINVTFDSASVDEFIGNVFSIDDDTLTITMNGLT
                     TTLVTDGFTEVNGLLQGEVNSITVENAPLVWEIADIGDVGASFKANLTPENSAYLEDV
                     IIAVSINETASTGVQTAYELAAVDASLDIVDIAYAFNIIGATLENGVDVMDATIVMTA
                     PESWVVSQGGPDRVRIFRYSEYDGTTEILEPIYVFDEIAGVYTFTALSPNGLSVFSLV
                     AVIETIPDTTPPLISDLLATPNPASINTPVLVTVTVDDSLTGGSTITNVEYSVDGITW
                     NPLIATDGAFDEMTESATVTLPGRASAEVFNVLVRTIDAAGNNALSDPLLVAVYDPNG
                     AFVTAGGWILSPVGAYMPDATSTGKANLGLNCKYKKGTTIPTGETEFHLKDADLKFKA
                     TSYEWLVITGPKAIYRGSGTINGNGLYGFQIVAVDGALSGGDDTFRIQIWEKATNTLV
                     YDNGAGSTTGDPGTTLGGGNVKIHK"
     gene            27047..27946
                     /locus_tag="PKM87_00425"
     CDS             27047..27946
                     /locus_tag="PKM87_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28561.1"
                     /translation="MPLFLSPVPPYDLILSCRVFAEGDPAIRRFQHGVLRQVIRVDAG
                     PVLVQVRDIGDGENPALAVDILSDDEVGENGRERAGNQVSLQLSLQDDLRQFYRAIRG
                     DPLMTGIAQSLYGLKAPQSATVFEALVTSIIEQQISTRVARIAEARMVRRFGEQMEVG
                     GMTYFSYPEPSDLAGGTIGDFRACGLSARKGEYIKNAAEMVAGGTLDLESMKNIRDSS
                     RIIEELCELRGVGRWTAEFVLLRGMHRIDTIPADDLGVRRATARVYFPGERISAEEVR
                     AIAECWGEWKGLAAYYLLASEHD"
     gene            28085..28720
                     /locus_tag="PKM87_00430"
     CDS             28085..28720
                     /locus_tag="PKM87_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2115 domain-containing protein"
                     /protein_id="HNQ28562.1"
                     /translation="MHTSSIQKECEELADFATGGELGRGIARLVLACSPRDLQRMRWN
                     FSKKIRDISPVYRKALEETITGYLHGTYQNVRLMNQQGVFSSMNERLPAHHPAYWRMV
                     SAHCATGNPEENRIRFLKYLLSGFCMFVQGIPGHPVGMPFPGGDRVESIDGVYYCPVR
                     TKANDVDAALCPFCPALQTPESGYLKPPVNASEHRKQEFLRNCFDRHHYNG"
     gene            28879..29190
                     /locus_tag="PKM87_00435"
     CDS             28879..29190
                     /locus_tag="PKM87_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HNQ28563.1"
                     /translation="MRETNRSVQASMADEARERAEKRLGEVKGFYSHLGIFIFINVLL
                     FLVNILTSPGQWWFYWVTIFWGIAVVFHGSRVFGKGRVFSSEWEEKKIRQYMEEEAQK
                     K"
     gene            complement(29283..29741)
                     /locus_tag="PKM87_00440"
     CDS             complement(29283..29741)
                     /locus_tag="PKM87_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ28564.1"
                     /translation="MEHTGKPAEILLVEDNPNDVELILHVFQWCNLSDRVHVAWNGEE
                     ALDYVFGTGPYRGRDIRGQPRVILLDLKLPKVDGIEVLRRLKEDEKTRTIPVVVLTSS
                     REERDIVQSYDLGVNSYIVKPVQFDEFANVIREMGLYWRMINQPPVKDEK"
     gene            29931..32462
                     /locus_tag="PKM87_00445"
     CDS             29931..32462
                     /locus_tag="PKM87_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ28565.1"
                     /translation="MILDQEKITRIKRLLKSRPKGLTISDISQMLKLNRNSVAKYLEI
                     LLITGQVEMRLYGNAKVYSLSQRVPISSMLKFATELILILDTEQKIIDVNDNFLSFFS
                     LQRDTLLGNDIRAAQIPGISGTILGELLTDSGRMDEGKQEISFTKDGREYFLALKVVP
                     TVFDDGSRGFTLIFEDVTLPRSYEEKLRINEARYRAIVEDQTELICRFKPDFRFTFVN
                     DAICQYVRRQKEEIIGTDIFSQVYEHDRDVVKKNILSLDHEHPFLSHEQRVLDPAGNI
                     RWQQWTNRAIFNDSGELVEYQGVGRDITERKHAEENLLVMNMAIASSINGIGLATLDG
                     RITYANASFLKIFGIPSLEDILGCHIETLGNPLNPGPGVKDVLKVLREGGIWKGKILV
                     IRKDGSRVHTHHTANLIRDKNGTPIAMMSSFIDMTEKVEAEKEIQIKNTAIESAINGI
                     IIFDADEHLIYANQAFLSIFGAKSLDEMLQNHIDEFWKIARETVPPVDDVKKMLIAEG
                     RWSGELRVKMPGGMLRFFQVSATQVKDERGAILCGMGTFMDITDQKVIEKALKTTHEK
                     LQDAIEFMPDPTFILNRERRVIAWNRALETISGVKSSDVLGSDSYQFAFGFLGGTRPI
                     LVDILDLPAHELAKSYPGIRRFGDSIFVESLIPGSHDEKGIYLWGKATPLFDRDGNII
                     GAIESFRDMSEWKKARETMHPHPEVEGWTPGSSGESRGGERESLACLEIAFAHLREGV
                     AILDRKARIRMVNSPFLALAKSSENVLIGKELSQFCHPDDNPLIREYLDYSGMDGDGS
                     PTVRIRVGERDDLFDAEISPIRGAGGVLLGHLVVTIPTFAATNQM"
     gene            complement(32453..32944)
                     /locus_tag="PKM87_00450"
     CDS             complement(32453..32944)
                     /locus_tag="PKM87_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF367 family protein"
                     /protein_id="HNQ28566.1"
                     /translation="MIPLYAFRDNSCDPRKCTIKRMERSGLVRVLSKISRIPRNTLLL
                     DPRAEQALSPADRPVRSITALDCSWEVLDKIPSGSWRRQRALPFLVAANPVNFGRPWR
                     LSSVEALAAALFILGEQDQAVLILGTAPWGIRFLELNEEPLGLYAGAKDSREILEIQG
                     FYI"
     gene            32976..33836
                     /locus_tag="PKM87_00455"
     CDS             32976..33836
                     /locus_tag="PKM87_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HNQ28567.1"
                     /translation="MYVLMCPCILHPELRARGITRGTDLQAFGKAIERCRMFEIEIVP
                     LPCPETLYLGSDRDPGTFLDRLNTTEFSRLIDQLEEEVRKVITTRGRPLCCIGVNSSP
                     TCGVDTTYYGADHAGPSRRPGKGVFLDRFPDLPMIDVLTFACFRIYLAAPLFSTAERR
                     FNAWLSEMLADHLFSVYLPQEAGEEGCCRSEEAQREMFSRHCEALSSSDVVVAVIDGA
                     DADSGTSWEMGYAYASGTPVISVRTDFRRAGAHEHVNLMLEQSSRVVRNEDELLRALQ
                     SPFLVEHRPF"
     gene            34043..35569
                     /locus_tag="PKM87_00460"
     CDS             34043..35569
                     /locus_tag="PKM87_00460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNQ28568.1"
                     /translation="MTSHRKSRGHPSPGIPPCDLNSGKMIEDSRKHAWNLDSIQASIR
                     SDRYIQILLLLTALGLLLRLYNLGFNSIWLDEAVTYNSSKLPFGVLWESIKTGSDFNP
                     PLFYMIESLMLSFGKNEITLRLIPAIAGIITIPVFYYLGKEFSDRNCGIICAALVTFS
                     PFLIFYSQEARAYSLLLLLIALSILCFFKGMKNGRTLQWVLFGLFSGIAFWTHFYSSI
                     VFGVLIGLGLLGTGISKENRLGKIKAIIYSLITFGLVTLPLLIVTAELYLVRTASPPT
                     FGIQGLNVISHAFTDLSGFNIIPTALFISLFGIGLLWAFLHEKMKGVSIALPVIAILL
                     ISYYLSYRMPFQTRFLIIMIPFYFLGIAYSSRAFCQIFRKNIVVYAIIACIFLVNVPI
                     LTEYYTVQTKENWRDFANEFEQFTTDGDVVVLLPSYMQQPLDFYYDSTRDHTKELGAS
                     NSGDLEKFMEEHAGKRIFVIMTGDILAVEPSGDTVGWLKENATFLGQYTGIYLFQISE
                     "
     gene            35655..36614
                     /locus_tag="PKM87_00465"
     CDS             35655..36614
                     /locus_tag="PKM87_00465"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNQ28569.1"
                     /translation="MNLRRLIYFTLAAVGIIILIIFIYRSGLYQNPGILLSINIPVLL
                     LAFLFTQMTIFLKIKRWQYLCVQYNFPISLRESTEVVIASFFPAGVSPGRIGDFMKAD
                     FMKIKYSVPFRDGISMVFYERIFELIIIFLVAVGILFIGFSAKYYIILEFTLFLLIFL
                     GVMYLFSDRFIHFFQNIARKKFRIFNSDEIKIRKISPRIAFNVSLYTILALGCEFIRL
                     WLVAYSFGYSLQIIHLSIFFSLAVLIGLLSQIPIGIGITEGSLSFFLMEMGVPYEIAV
                     GIAVIDRAISMYFVILIGFFYYHRMRTYYVNEGESVNNHSRIQ"
     gene            36574..37314
                     /locus_tag="PKM87_00470"
     CDS             36574..37314
                     /locus_tag="PKM87_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ28570.1"
                     /translation="MKGKASIIIPVFNEEDILAEYPVNLFPVLEDIFIKYSMDFEVIL
                     VDDGSTDRSWLKISELQRLKLYLKTYKMPQNVGMGGAVRKGIELSEGEIIITLDADLT
                     FKPKEIDILLTEYVKNPVDCVSGSPYLKDDLMLDVPLVRKILSKAVNYIYCLELGQRI
                     TSVSPIFRLYRSEIFSTIPLTSNNFEVNAEILSKMILSGYTVREVPAHLFKREHGQSK
                     AKIWKSIRNHSVIIGKIFKVKFLKGNWN"
     gene            37341..38132
                     /locus_tag="PKM87_00475"
     CDS             37341..38132
                     /locus_tag="PKM87_00475"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ28571.1"
                     /translation="MKNNTIPIESLSDEDLYRLNIYHRTDDEHSFKQLHSLYGRVNAA
                     QDATIINQVYGRRVLDVGAGYGSLSRRLLDSAFEVYSIEPNKDVRELALAWHNVKELP
                     YSIYDTPFSSHFFDTVILRECVEHLDMPRAMKEISRICRMRLLIFQTNLNPLISLLRG
                     ILGHHEYNPQNLAYYRNCANLAGFSVQNVLFRDILAFPLSGGYVSHSVIPRQPFIEHS
                     IIQIDEKLSRFFRFFGMDSIVSWRFLLIADKAILYRREHEKVKTQ"
     gene            38149..39111
                     /locus_tag="PKM87_00480"
     CDS             38149..39111
                     /locus_tag="PKM87_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HNQ28572.1"
                     /translation="MPNLTVAVIGPEGYAGHLGKKGTVSDITFYNQKRDETTVTYIEP
                     SRYPEKLSSLFFSISMAGMALLVVDEINAAFGECVLMLDMAKVRKGVLVLRNYLSPEQ
                     VAPLIKGTVVEHFQVHSDDHGQLRELFLEEAANASQPAEGSKGGSVPVDHHFPVKGVG
                     IVVLGGVAEGTIHAHDQMKVLPTGKVAHLRSIQKHDDDVSEASAGDRVGLALKGVEEV
                     DLDRGYVLSTNPSLVCSSILTGRAELVRYWPAPLKEQMIVYLGHWMQFIPARIAFVNN
                     SGDWKRPEITLRSEKELVYPPGAPILIHYLEGGKLRVVGRIVPA"
     gene            39218..39790
                     /locus_tag="PKM87_00485"
     CDS             39218..39790
                     /locus_tag="PKM87_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNQ28573.1"
                     /translation="MALGDLIPWIAGGIILLVIIGLVLYFVGIYNRFFSLKNSSEATL
                     GQIRVAMKKRLDMIDQLLGAVKSYAAFERETFEKVTAMRASVGSAGPGDLNQIERESR
                     SLLGRLFAVAENYPDLKTNATVMNLMDAVRGVEDEIARQRYTYNNISQQYNTLRDVIP
                     SNIVARILGLSKLEYLEFEEAIQTPPKIAF"
     gene            39803..41620
                     /locus_tag="PKM87_00490"
     CDS             39803..41620
                     /locus_tag="PKM87_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNQ28574.1"
                     /translation="MGETRELLLLIVLGFVLGAAAVLVSLAIPVLLEGDLVVDQYRAT
                     LHEDGTFQEFYTYEVKSSGQYRMLYRYWQDPLSSQPLDSPYIWFVEMDYPAGNVGYMK
                     DWAGKVTLFGDDNPLYIRFVEDRAERNEVGIYNPGYFPAGKYTVSYTYVLHPPIEYDK
                     IDAHLNLRLVDEHVPFRTVEIRIPERYAGEVFPHPPGLAVTREGGGVLIKGSVAADET
                     LGFELLFPAAEAAGIPGFSSYVEDVRGKTRAANSIIQQIPLLGAWALYGSGVVMIFLT
                     PLLLLLIHRRFGREKEFTVPEFLSFTPNPAMKPWAVNLLFKGDAIEFDQDGYYATLLD
                     LHRRRVITIREKTDGKGITINVNEWQSEEPYEQRVLDFLRNVEEEGIIDSDSLQKMAV
                     LARSDREVEKRMLGYQQQLSGVTKKSDPRLISQYIVDGRDHLFPLLFVGIAFCAVSVM
                     VLIVVPVLAAILGPAVVLWAGVIVQSGVALAFPSTLFGHWKDDRYKEKLEWDSFARFL
                     SDLALIKQYSPQDISMWGEWLVYGTALGVGEKVEKAMETLHISLPEAGVPLAMGLRTA
                     FIPVLAFSAPSHGGSGGGFGGGGSFGGGGGFGGGGVGGR"
     gene            41651..42103
                     /locus_tag="PKM87_00495"
     CDS             41651..42103
                     /locus_tag="PKM87_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNQ28575.1"
                     /translation="MDCTEGRIGRVFVLRIDHGEDILETLLQCIREKEIFCGTVQVLG
                     ALGKGQMVTGSEEPVLPPLPHWEKVDGGWDIFGVGTVSWGEDGPHLHLHSAVGRGTRS
                     LAGCLREQARVYIVVEAVILEINGIHTVRTHDETTGLTLPRFLPGNPP"
     gene            42100..43932
                     /locus_tag="PKM87_00500"
     CDS             42100..43932
                     /locus_tag="PKM87_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNQ28576.1"
                     /translation="MKRRQPKFLPMSKGEGKGLRIDQFDVILVSGDAYVDHPSFSTAI
                     IGRVLWDAGFTVGVIAQPDWKGNDDFLRLGDPRLFFAICPGNCDSLVNNYTAAKKRRS
                     SDVYSPGGIPRRPDRAGIVYTDRVHALFPGIPIVLGGIEASLRRFAHYDYWSDSVRHS
                     ILADAPADLLVYGMGEQQVREIALRLDKGEPPVSLTDIRGTVYRVPPAQYDGTCGFPA
                     LEIPPFSEVAADKVAYARAFATHYREQNPLRGRAVVQRHPKTIIIQNPPALPLSTQDL
                     DAIYELPYSREVHPSYTLPVPALEPVRFSLTSHRGCFGGCSFCALTHHQGRIIQSRSI
                     DSLVKEAIRLTRMRGFSGIISDVGGPTANMYGMECARWQTEGPCPDRACSVGCPNLLA
                     SHRRQMALLRRLREVPGVRRVFIGSGMRYDLIVADPEDPLPEICAHHISGHLKVAPEH
                     TSPAVTALMGKPGKEVFERFLASFSKCQEGKEKKQYILPYFMSSHPGCTVGDMVDLAV
                     FIRDHDLYTEQVQDFTPTPMTVSTCMYYTGLNPLTLDPVHVPKGHEKQVQRALLRYRD
                     RHVRGLVEEGLCFAGRKDLLQKGRGGLLSSREKKGKSGMRQEEC"
     gene            complement(43929..44378)
                     /locus_tag="PKM87_00505"
     CDS             complement(43929..44378)
                     /locus_tag="PKM87_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNQ28577.1"
                     /translation="MNDLEEFELTLQEIVSRGGEEIAEAWMKDIEVEYGRAPLIFKRM
                     AERPEVLISHLLYKTAVIKTSAIDPKYTELISMAVGAALRCPHCTSYHMQAAAKKGAT
                     REEILEVILIAGMISNSSVLANAYRIFDEKMSRCLPCENRGIDIPER"
     gene            44539..46134
                     /locus_tag="PKM87_00510"
     CDS             44539..46134
                     /locus_tag="PKM87_00510"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNQ28578.1"
                     /translation="MVNWSPFLHSTCLVLLVALCFGAIGAVQAAPMRYDLTEKFSSHR
                     MQDNSPGKMPGGNMFAPVTYSRADIASAAAPWKRDHSSPGIVMRESGMGETLPIVSGT
                     QKFNFTIPERNTTTVPGRNISFNRPVGKASLVSGTGTVVYMDLEGGFYGIIADDGARY
                     LPDLLPASCKVDGIRVKFTGMEKSGEASIYMWGTSLRILTAMPLGEEVKAEGRIQYID
                     LEGGFYGIVAADGRSYLPLNLNKEFQVDGMKVSFTARTVPDTLGIYMWGIPVTVISIS
                     ARQGEDKSASQSLIGPWTLSGMARGAAVAPVIQGTTITAEFTSDGRVSGTAGCNLYFA
                     PFEQSGTTLRIGPAGSTEMYCFDASRTIMKQESTYLQLLGEAASWKISGGNLVISDAS
                     GQEILVFSPGIADASDRTEPLVEFWRTGGFAGMNDHLEVYPDGSVSLTRKEYSVGFTL
                     SEADLGTLNSLLQESGFMALSSEYRAAPGSADLFLYQVRAHGKTVLAEDTVIPGSLQP
                     LIDELVRLVEENAPDDIAPPRIH"
     gene            46306..46854
                     /locus_tag="PKM87_00515"
     CDS             46306..46854
                     /locus_tag="PKM87_00515"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HNQ28579.1"
                     /translation="MRGVGMKVWRNIHWLMLFVAMAVLLSFSGCISPQDFVAEPGPGP
                     TGTLTPATTPTAGIPTEEGGIGVLPVVRGGTSQYFVKMPYGYMVTSPTTGVRLSVIEI
                     KDDLDATGQRVISGKIKNDENYRIDHITVYFNLYNSNGNLIGNAYASVNSLGPGKVWR
                     FTTNPFAYKDYQYYEMAGTFTA"
     gene            complement(46897..47834)
                     /locus_tag="PKM87_00520"
                     /pseudo
     CDS             complement(46897..47834)
                     /locus_tag="PKM87_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314778.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 7 protein"
     gene            complement(47831..48412)
                     /locus_tag="PKM87_00525"
     CDS             complement(47831..48412)
                     /locus_tag="PKM87_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 17 protein"
                     /protein_id="HNQ28580.1"
                     /translation="MEAVEYFEVECPEKAGGEYYRRIADVVLLDHNLIRVIQKLHIFI
                     DPRVPLFIATGITRKLPGTVKVRDFSDVNRKERKVTISIGDEAYLAPLLRILWEKFGK
                     ERVEQPDRFTIMLDIGDDESEGLEEIPVFDPSESIYKDLIYALQVIQPEGFKVRRQYY
                     GNGRFYLVASEDTLPEDIEDMVREKFSLMGERL"
     gene            complement(48413..49648)
                     /locus_tag="PKM87_00530"
     CDS             complement(48413..49648)
                     /locus_tag="PKM87_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 15 protein"
                     /protein_id="HNQ28581.1"
                     /translation="MTRVRIAQLSCGPEYSGVQKEIEAAAASVDAEVFFPDLKLADIR
                     HSFNEFGLEVRSPDLKLAIARAKALVEGRVEADAVFIATCFRCAEAAIVRNELRRYIH
                     ENSRLPVVSYSFTERTTAGTLLTRMEALTTIARRRALLAREVQQGLTMGVDSGSSTTK
                     AVVMRDNEIIGTGWLPTTGVLQSADDAVSQALSAAGVKRDDLQAIGTTGYGRFLVGEH
                     LKADLIQEELTVNSKGAVFLADTQRGPSTVIDIGGMDNKAISVQDGIPGTFTMGGICA
                     GASGRFLEMTSKRLGVDITELGPLAMKGLSRNVPMNSYCIVFGTQSLVNALAAGSSPE
                     DVAAAACHSVAEQVFEQQLQEVDIKDPVIMVGGTSLIQGLVRAMGDLLQTEIVVPHHS
                     QYIGAVGSALLASGFIKGD"
     gene            complement(49645..50094)
                     /locus_tag="PKM87_00535"
     CDS             complement(49645..50094)
                     /locus_tag="PKM87_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 5 protein"
                     /protein_id="HNQ28582.1"
                     /translation="MAKVFLYPTTSLILSDLVARFGHKPLSAALKIRERIQTPGIDSP
                     PLQITPEEPKKGLKWAAVEVPSGVRGRMSLYGPMIDEAEAAIIINNADYSFGCMGCAR
                     TNELIKYLIRKRDIPVLDLEYPTTEDEGVEFVAAIKKFLDTLGAGSP"
     gene            complement(50096..50554)
                     /locus_tag="PKM87_00540"
     CDS             complement(50096..50554)
                     /locus_tag="PKM87_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 6 protein"
                     /protein_id="HNQ28583.1"
                     /translation="MTAYKPRYAGSVTKYVFVESPEMTPSDLAIRAYEISQGVMIKET
                     CFGLQVTGKEEDVERIIASLRALDPTHIFVKDRGFPPGDPRRCRANLGGARPGYFGHE
                     FEMGLIQNISRGLASLPSRARDDLPHPPAPSKEPRLDASRLKKIIESQES"
     gene            complement(50551..52080)
                     /locus_tag="PKM87_00545"
     CDS             complement(50551..52080)
                     /locus_tag="PKM87_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 3 protein"
                     /protein_id="HNQ28584.1"
                     /translation="MIKILLDGERLETGDGARLRDLLPDHDKACCVGIIRPGARESAT
                     TGSMRLDTSAGEVVVELNPAGQAIFGSASLQFPLSLHWQDRYAAAFGPFHSSFTPART
                     ASLYERGDVVLGCGGYDPHRSYLIFCRSRHTSDHGAAAGGGVIGKVVSGRGVLDRWST
                     GDLVNTASPVLNWADTSRSFTTTDPDTVLEDGMEVISHLRITSFGYSGSEVTSTAAAE
                     SIEHLLLALEDAHFVVGRSSSTHIRDDRKAGTEVAYQVKQPRREGAVSVRTAGRSGGS
                     VYIYIQDLPGTPQHTVVGQVTHGLELARLARDGDVLSARVVPSRFDLIGLPLEQAIDT
                     AEQRGIRILGELEGRDLVVVGQEPATTIESLAAGAVELSVVPLEKVIDITLDDDAAPA
                     SCDIFRHLTGLHLHDVGRIPFFFNFEDVFLFKPKIPRGVNIIPENTPTDLVPSGALAI
                     TNDSRKGSGLVGVRTSENSEFGPTSEPFEGTNIIGKVLDLEKLAALQEKEIVYIRERQ
                     P"
     gene            complement(52091..53704)
                     /gene="atwA"
                     /locus_tag="PKM87_00550"
     CDS             complement(52091..53704)
                     /gene="atwA"
                     /locus_tag="PKM87_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl coenzyme M reductase system, component
                     A2"
                     /protein_id="HNQ28585.1"
                     /translation="MNPLITVENLCMDFDGKRVLHNISFEIGEGEVLGIIGRSGAGKT
                     VLMHLMRGVEQPPTEGKIIYHVAVCDSCPFLGVGSLAGKPCPGCGKTLKARDIDLWNE
                     PDEGMRRRLMRHTAIMFQRTFALYGNDRVIENVLHALDDIGYPQEKAINRAADLLDQV
                     RLSHRMMHIARDLSGGEKQRVVLARQLAKEPFLLFADEPTGTLDPETAHLVHSMLIEA
                     AETNHMGMVVTSHFSQVINEVADRAMLLAEGKIAALGSPEEVIRTFMADLSDTEEFEV
                     PSFGGEIMIARDVFKRYISVDRGVVRAVNGVTFEVKEKEIFGIIGKSGAGKTTLSRII
                     SGIIEPTTGEMIIRIGDDSVDMTKPGIENRGRAKGYIGLLHQEYDLYPHRTVLDNLTD
                     AIGLEFPKELAMRKAIITLKMAGFNETKSREILDRLPGQLSEGERHRVALAQVLIREP
                     RMVILDEPTGTMDPLTKIDVKHSIMHARDEMDETFIVVSHDMDFVRDICDRLALMRGG
                     KIVQIGQTAEVLASLTEEEREVMGKAVTS"
     gene            complement(53781..54590)
                     /locus_tag="PKM87_00555"
     CDS             complement(53781..54590)
                     /locus_tag="PKM87_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNQ28586.1"
                     /translation="MSIAVVFDSAGTLLRTYRVAKDVLKGELLPGIETVSLTFSSPER
                     VLVVIHVHSREVIETPPEVPLSRYLVEHQVGFGVSCARKVIVADEVAAALYQSPHGTI
                     GDLQDCIRNVWHVCKRESVVAMNSGVIVNMGLSGIEFTVTTGGRPFEGARESISELHR
                     MGIPTYIASGDRVTKLEKMADFLGIPRDRVFGNATPTVKAQIVADLQEEYDRVLMVGD
                     GINDICAMKKSDVAILTEEQSGERHPELYQAADHVVKNVREVVAIVKRLVH"
     gene            54654..55586
                     /locus_tag="PKM87_00560"
     CDS             54654..55586
                     /locus_tag="PKM87_00560"
                     /EC_number="5.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0046033 - AMP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphate isomerase"
                     /protein_id="HNQ28587.1"
                     /translation="MLADTAEKIKRMEIRGAGRIARAAVEALDEYSGALAEGSLQGYL
                     ERMDEAAALLIATRPTAVSLPNGVRSVMSVLRQQFSSVEEAKQAFHERCRAFIVASGQ
                     AVQRIGEIGSRHIRDGDVLLTHCNSEAALACILEAKRQGKEISVFATEVRPRNQGHLT
                     IRTLDEAGIPTSFIVDSAARYFMNEVDLVVVGADAVSVNGAVVNKIGTSQIALAAREA
                     RTRVMVAAETYKFAPRTILGEYIEIEERDAGEVLSPALSRQLPHVRVRNPAFDVTPAG
                     YVDLIVTETGAIPPQMAYIIIRDVLGWDIGEYSG"
     gene            55712..>56193
                     /gene="dapF"
                     /locus_tag="PKM87_00565"
     CDS             55712..>56193
                     /gene="dapF"
                     /locus_tag="PKM87_00565"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNQ28588.1"
                     /translation="MELHFTKLQGNGNDFILIDEYKGVAIPDEMKAEFAALYCDRRFG
                     IGGDGVLYLSLSQKADLKMRLLQPDGSEAEMCGNGIRCFAKYAYDRGYVRKSCTVETP
                     AGIIPVEMNYQDDSFFATIGMPEPKFMRKDIPATGEGEYKEEIEGFEVYAVNTGVPHA
                     V"
BASE COUNT        12322 a        15514 c        15496 g        12861 t
ORIGIN      
        1 acccacctct tcgggccggt ctcgtcccgc cgcctgggac gatctcttgg ggtagacctc
       61 gttccctaca aggtctgttc ctttgattgc gtatactgcg aggtggggtc caccaccatg
      121 aggaccgtca cacggcagga ctttttccct ccatccgaag tggaggagga gcttgtgcgt
      181 tttttttctg gagagccgtc ccttgatttc atcaccctct cggggtcagg tgaacccaca
      241 ttgtctctct ccctcggaag ggtggtcagg tttgtaaaat ccaggttccc tgcctatccc
      301 ctggcagtcc tcaccaatgg aagcctcctc tgggacagcg atgtgcagca cgagatcctt
      361 cccgcggacg tggtgctccc caccctctgc accgtcgacg aggagatatt ccagaagatt
      421 caccggccag cacctggcct caccatcaaa aagatcgtag aaggactccg ggacttccgg
      481 aagatttacc caggagagat ctggcttgag gtcttcctca tcccgggcat caacatggac
      541 cgggatcacc tcagtgccct tggagatgag atcaggagga tccatccgga cagggtccag
      601 ctgaacactc tcgaccgccc cggaacggaa gcatgggtgc gcccggcgag ccgcgagcag
      661 ctggccgatg cggcatatca tctcggcctt cccggtgcgg agtccatcga gcctgtgccc
      721 tatcaacgtt cccaggagca aatttccgca gaccctgcat cgatgattgt ggagatgatc
      781 agtcgccgcc cctgcactgt agaagatatt gccctgacga cggggctgca tctccaggag
      841 gtaggaaaac tcctgagagc actctccagg gatcctcgtt tgatgacgaa gagagaggag
      901 cgggggatct tctattcctg ggtcggtgac tgatcgtccg ccaggcgata gtctgtgagc
      961 ctgcgacgca acacccgccc gatatccgca accatccgct ccatctcgcg gggatccagg
     1021 aagtcggaac cggaggctcc tgcatcgcct gaaacatcgt ccgagaacat ggtcccggca
     1081 agatcgtttg cacccgcaag gagtgccacc tgaccaagtt tcaccccaag cttcccccag
     1141 gagacctgga tgtttttgaa gttgtcaagg aagaggcggg agactgcaat gaggaggaga
     1201 tcctcccttc ccgtcgcacc tggaggagct ttcccctcga cgtagagccg ggtgttctgg
     1261 tggagatagg agagcgggac gagttccgaa aatcccccgg tcccgtcctg gatatcacgc
     1321 aggatggaga ggtgggcgat cctgtccctg ggggtctcgc aggagccgta catgatagtc
     1381 gcggtggtgg gaattccaag gccatgcgcc tcacgtatga tccggaccca ttcgtccgtc
     1441 ggaacctttg ccgggcagat gatttctctt accctgtcca caaggatctc tgcggcggta
     1501 ccctggagag atccgagccc cgccgctttc atagcaagaa gcacctcgcg tgtggagagg
     1561 ccggagcggg tggcagcatg gctcacctcg tctggagaga atgcatggac gtggatctcc
     1621 ggtgcgacct ccttgaccca ggagaggatc tcaaggtaac gcgcggcatc gaactcaggg
     1681 tttacgcccg agaggaggca tatctcgctg accttgcggt cgagggcgag cctcaccttc
     1741 ttcccaacgg tatccctgtc gtcacaaaac gccccctcgt cagttgcact tctcccgaag
     1801 gcgcagaacc cgcagaggtt cttgcagatg ttggacaggt gaaggttctg gtttcggaca
     1861 taggtgatca ccgggcccac catctcttct cttgcctgat ctgcggcgct gcatacctgg
     1921 aacacctcgc ggcccttcac gttgagaagg aacagcgcct cttccctgtc gaggcggtgc
     1981 cctgccctgg catcagaaag cagggtgcga agatccatcg agagagacat tcccacctat
     2041 ctcttccctt tcgccagttt atccctcttt tcgctccttt cccccttctt cctcgcatag
     2101 tactcccaga ccagcatgag ggcgataagg atgattgcga ccggcacgat attcaggggg
     2161 aggtatccga ggagtgggat cgataagagc gcctttccaa cgatccattc atccttcacg
     2221 ggcatgatga cctcgccaga taccgaggag aggtagcctc cctggtcact tacctggttg
     2281 ttgtccccct tggtgatgta gccggcatgc gggctgacaa gggtggtctc aaccaggtaa
     2341 ccgttcatgg ggtctgcatt ctggggagtg acgactgtcc cgtcctcgag gacaaggctg
     2401 ttgttctgga tggtcgccgg gaggtagcgg gccggggtcg cctggccgcg gtagaggttg
     2461 taatacttgg gaatgttctc ccccgcatca atgcggtcca ttgcccggtg gatgataggg
     2521 tgcactccct ttgtcaggaa cgggatgtag acaggagggt taggggcatc attcggccga
     2581 tagatgagaa tatccccgta gtccccgtat ttcaggtagc ccttctcaat cccgctcgtc
     2641 cagctctcca gccccccgaa ccggtcagcg ctgaccacga agaccaggtc ccctacctgc
     2701 atgtgcggga ccatgctctc ggactcgatc gtcacgactg cgggccatgt cccggagacg
     2761 agaaagagcg caagggcgat tccccccacc accgctgcga cccacgcaag ctcccttgcg
     2821 agcgaaaccc aggggttctc gcttttcctg aattgttccc acagcccggg tttattctct
     2881 tcgggatcgt tgcgcgacat tgttggactt tgaatatttc ctccggcgcc ttataaggga
     2941 ttgcacgctg cctgccgcag gccggcggtt cgcatctcag gataatttcc cgccggaggg
     3001 cacggtccgc actccatgga catggagtga aagtcctata tccggcacag ccaatattga
     3061 tgcaatgctc gatgcggcca ccattgtgca gcggttcctt gcgaccaaga tgcaggtgca
     3121 tcccgaggtg gtccgctatc tctccgagaa ggatgacccg ggcctgatcg accagatcat
     3181 cgcaagtgtg ccgggagata ccattgtcgt atccctccag cacattcccg ggctccacca
     3241 ggaccgtgac ggcgccaggt tcctggcaga cccttcatgc gaggtgctcc ggggggccgc
     3301 aggaacgacg aagagcatct cgcacatcac tgattactgc cattacttca gagaccggta
     3361 ccagcgcctg ggagagtata tcaggccacg ggcggcgccg atgcccatcg aggcgctgaa
     3421 gaagggggcg cggtaccgcc aggaggcctg ctccatcatg gggctcgtga tggagtccaa
     3481 ggcgaccacg aacggccacc gcatcgtgga gatcgaggac tcgtccgcgt cgatctccgt
     3541 cctgtttcac aaggaacgcc cgcagttcgg ggaggccgag accctggtcc cggacgaggt
     3601 cgtggcggtg agggggacgc tctctggcga cggccgcctc ttctttgccg agcagctctt
     3661 caggccggac atccccctca cccatgcacc cttcaggagc caggatcccg ggtctgcgat
     3721 cctcatctcc gatgtccacg ttggaagcaa cacgttcctc gacgaggcct ggaaccgctt
     3781 tgccgactgg ctctccggct cggacgcatc ctacctcctc atcgcagggg acctcgtgga
     3841 cgggatcggt gtctacccca accaggagca ggagctgacc atcaccaaca tctacgagca
     3901 gtacgaggta ttcgggtcga tgctccggga ccttccctcc cgcatgaaga tcgtcatctg
     3961 cccgggcaac cacgacgtgg taagggctgc ggaaccccag cctgccatac cttctgagtt
     4021 caccgcgaac tttccctcca actgtatcat ggtggagaat cccgcgctcg tcagcctcca
     4081 gggagtgagg gtgcagatgt accacggccg gtcaatcgac gacttcatca gcctcatccc
     4141 cggcgcctca tacgagaacg ccgcacccat gatgatgggg atgctgcaga ggcggcacct
     4201 ggcccccacg tacgggaaga ggaccccgat cgcggcaggg aaagaggacg ccctgatcat
     4261 agaccccatc ccggaggtgt tgcacaccgg ccacgtccat atcatgggaa tcggcgatta
     4321 ccgcggagtg ctgtgcgtga actcgggcac gtggcagggc cagaccgcct tccagaagca
     4381 gatgaacatc acgcccacgc cagggagggc ggtggtcctc gacctccaga cactgaacac
     4441 acgggtcctc gatttcacct gattgcggct gcattcccag gattctgtgt ccgggtgcat
     4501 gtatctgccg gcgcggcgaa ggtccatcag aaggccccct ttcccaggtt aaaccccctc
     4561 ccctttccgg cggccggata ccacttcaac gggtttcatg tagtttttta tgtgcccggg
     4621 tcgattcatc tcagataatc atctggtatg cgtggagaat ggtacattct ccggaggttt
     4681 tacaggtatg gatatgttct tcattgttcc cgtctgtgcc ctgctgggcc tggtctttgc
     4741 cgggtactcc ttcatggtca tgaagcggga gggcacaggg accgaactga tgcagaaaat
     4801 cgccggtgct atccggcttg gggccatggt gtatctcaag agccagtaca aggcgatcgc
     4861 ggtattcatg gtcgtgctgg caatcgtcct ctatctcgca ccgggcatcc acccgctcac
     4921 gtcggtggca ttcctggtag gcgggatact ttccgctact gcgggattcg tgggcatgac
     4981 cgcggccacc atggccaacg tccggaccac caacgccgcg cgtgaaggca tggcaaaagc
     5041 cttccgggtg tcgtatgcaa gcggaacggt catggggctc accgtggtcg ggctcggcct
     5101 catggggctc tcgctcctct tcctgaccct gaacgcgtta accaccctcg ggctctccga
     5161 gatcatcaac atcctttcag ggttctcgct cggtgccagt tccatcgcac tcttcgcccg
     5221 tgtgggcgga gggatcttca ccaaagccgc cgatgtgggt gcagacctgg tgggcaaggt
     5281 tgaggtcggt atccctgagg acgatccgcg gaacccggcg gtcatcgccg ataacgtggg
     5341 ggacaacgtc ggcgacattg ccggaatggg cgccgacctt tacgagtcgt acgtcggatc
     5401 cattgttgcc acgatgctga tgggtgtctc tcttgcagct gcgacacagt tcgtgggagt
     5461 cgcttccctg aacctcgtgc tcctcccgat gttcatcgcg ggccttggga tcatcgcatc
     5521 catcatcggg agcttctttg tcaggagcaa caagaacgag agcagcgcca tccataaagc
     5581 cttcaatgca gggaccttcg caagcctcat cctcactgtg atcgccgcgt acttcctggt
     5641 gacgatggtg ctcggtcccc agtacatcgg ggtctttgtt gcaaccattg ccggcctcgt
     5701 ggccgggttc ctcatagggc tcatcacgga gtattatacc tcctacgagc gcccgccaac
     5761 ccggaagatc gccaactcct gcgagacggg tgcggcaacg aacatcatca ccggccttgc
     5821 cgtggggttc gagagcacgg tattccccgt cgtgattgtc gtcgtcgcga tcctcatcgc
     5881 ggcacagcag gcaggcctct atggcgtggc tatcgctggt gtcgggatgc ttgcaacgct
     5941 cggcatcacg ctctcggtgg acgcctacgg gcccgtcgct gacaacgccg gcggtattgc
     6001 cgagatgtcc caccagccaa aagaagtccg cggcatcact gatacccttg acgcagtggg
     6061 gaacaccact gccgcgattg gcaagggctt tgcaatcggc ggggccgcgc tgaccgcgct
     6121 cggcctcttc gccgcgtact cccaggctgt cagcctcgag atcgtgaaca tgctcgagcc
     6181 cgtagtcttc gtcggtgtcc taatcggcgc gatgctgccg ttcctcttct gctcgtttgc
     6241 gatgaccgcc gtcgggaagg ccgcaggata cattgtgcag gaggtccgcc gccagttcaa
     6301 ggagataccg ggccttatgg agggcaaggc cgaacctgac tacagtgcct gcatcaccat
     6361 ctccacgaat gccgcgctca agcagatgat cgcccctggc atcctcgcca tcgccgcacc
     6421 cctcgtgatt ggccttctcc tcgggacaca cgccctcgca gggctccttg tgggctccat
     6481 atccaccggc tttatcgtcg ctgtgatgat ggccaacacc gggggctcct gggacaatgc
     6541 aaagaagtac atcgagcagg gtcacatggg cggaaagggt tcgcccgcac acaaggcggc
     6601 cgtcaccggt gacactgtcg gcgacccctt caaggacacc gcgggaccgg cactcaacat
     6661 cctcctgaag ctgatgtcga tcgttgcggt ggtctttgca ccgatgttcc tcatctaatt
     6721 cttttttttt taaaggtgcc agagctctcc ttgttctgat acaatggttt tccagcacgg
     6781 ctggggatac tggaagagga tggcgatcca gagcattatt gcgtgagatc aaagggagcc
     6841 ggggcccgca gaacaaatag cccaagtgac caatggtgta aaaatgcctg gccgtgcccg
     6901 tggctctcag ggctttccga tcccgagtcc tgcgaggaac atccggtgca gcctgatatc
     6961 attccctagt tcgggatgga acgagagtgc catatgtggc ccctttctca ccgcaacgat
     7021 cccggcatct atcctggcca ggacctcgac cccgttccct gtcccggtcg ccaccggtgc
     7081 gcgtatgaaa aatgcgtggt acggcgtatc cagccctttt atgggaatct cggcctcgaa
     7141 ggattccttc tgccgcccga aggcgttccg gagcaccgtc atgtcaagga gcccgagcgt
     7201 cttcacccgc gggtcgtcca cccggcctgc catcagcacc atgcctgcac aggttgcaaa
     7261 aattccgcct tcaaacctga tcaacggttc atacagcccg ttcttctgga tcaaccggga
     7321 gatagtggtt gattcgcctc ccgggatggc aaggccagag agcccggcaa ggtctgcagg
     7381 ctgcctgacc gggaccacct ctcctccctt cgccccgatc tgcgagagcg ccgccataaa
     7441 acccctgaca tgctcgctca catcgccctg gagggccaga acgccgattc taacgcccac
     7501 ggtactggag cacctcgtcc tcgcgcaggg tatggacgtc gatccccttc attgcctgcc
     7561 cgagcccctt gctcacctcg gcgatcacgc caggctcgtc gaagtggttg accgcctcca
     7621 cgatcgccct tgccatcctc ctgggatcgc ttgagtggaa gatgccggat cccacgaata
     7681 cgccgtcggc tcccatctgc atcatcaggg ccgcatccga tggcgtcgct attcctccgg
     7741 cggagaagtt tacgaccggg agcctgccca tcttcacagt ctcaagaagc agctctgcgg
     7801 gagcctcgag ctcacgggcg cgggctacga tctcctggga gttcttcccc tggagagcgc
     7861 ggatctcccc catgatgttc ctcatgtggc ggaccgcctc caccacgttc ccggttcctg
     7921 cttcaccctt ggtccggatc atcgccgcac cttcgtgaat ccggcgcatt gcctccccca
     7981 ggtcacgggc cccgcagacg aacgggaccg taaagctggt cttgtcgata tggaactgct
     8041 cgtcggcggg ggtcagcacc tcgctctcgt cgatcatatc cacgccaagg gactcgagga
     8101 cctgggcttc tacgaagtgg ccgatacgta ccttgcccat caccgggatg gagaccgcat
     8161 ccatgatctc catgatgatt gccgggtctg ccatccgggc cactcccccg gccttccgga
     8221 tgtccgcggg aacccgctcc agggccatga ctgcgacggc cccggcctcc tcggcgattt
     8281 ccgcctgctc cgcgttgacc acatccatga tcacgccccc tttctgcatg gacgcaaatc
     8341 cccgcttcag gagctcggtg ccaaatctca gttcctcgag tttcatatgc ttatataagt
     8401 gggatgagca gggccaaaaa cattagtacc ataagtagca gggtgacgag tatcgtgcag
     8461 gcccgcaccg ccgagagtat ctccggccct gcatcttcaa tcgtccggtc cccggggccg
     8521 atggaatata cgccaggctt ttcgaacctg atcccggtcc cccctgccat caccgccatg
     8581 gggaggccac cattaaaccc gggccgtttt ctcccatcct ttcgcacaca atccagcgcc
     8641 ggtgcgaacc tccccctgca tgcgaagtaa caaagaagga tgcccgcggt cagccgcgct
     8701 ggtatgtagt tcaggacatc gtcggcccgt gccgaaaacc agccgagccg ctccctatgg
     8761 tcgcggtacc cgagcatggc atccatggtg ttggctgccc gaaacgccgc ggcaccggga
     8821 agcccgagga gcgcgtagta aaagagcggt gagacaatgc tgtcgacgag gttctcggat
     8881 accgactcgt acgcggctga gtggacctgt tcccgggaga gtactgaagg atccctgctg
     8941 acaaggaact gcacctgtga cctaccctca tcgagaccct gcgaaagcgc attctcgacc
     9001 gatagggcat gctcctcgag ggaccgccat gcgaggcacg acttcaggag tacggggccg
     9061 gccacaatca ggacaagcca cgggagaaag atctctgcga gtatgaaggg cagcgagaag
     9121 agaagaaccg tgaatgccca cagtgccgcc cctgcggcac gctggagaca ggggggatag
     9181 agatcgggcc ttccccacca cgcgatgaac gtcccgagga gcgctacagg gtgcagcctg
     9241 ctgtgcgggt cgcctgccag gcgatcgaac aggagtgcaa gcggcagtac cagggggcgg
     9301 atccagaaca tgcgacgagt ccatccagga gagaattctc acacgatctc acgcggaaag
     9361 cagaataatt attcgaatat tgtgcgtccg aacattgcga cctgcgatgc tgcagtacga
     9421 agtacgaggg aaaggcccgg gggctcggag ggaggcactg atctattgaa gagcgcgttt
     9481 tttataaaaa agggatttga atcggggtat tttagacctg caccgggggg gccgggttcc
     9541 ttccttactc ttcgtgcgtc ctgggaagga ggtccgggat cccttcctca atgggatact
     9601 ccaccctgca ggccgcacag tagagccctc cctccaggat ctcgacgtcg ttctcttcct
     9661 tcacgcggag ctccaggtcg cccttgcaga ccgggcagca gaggataggg aggaggctcc
     9721 tcttcatcgt tctcccctga tatcgatgat attcgagaca tccggcccgg tggagatgag
     9781 ggtaatgggg cagccgatgt ccttctccgc gtgctccagg aactcgagtg ctttctttgt
     9841 gagcttgtcg tagttggtga gaccgaaaca tgccttgtcc acgcgatcta tgccggtaat
     9901 ggccgcctgc gtacacccgt tgaccatcgc cgagtagcgg gccatcgccc cgtcccactg
     9961 cccgattcgt cgctgccggt gagtcacggt accaaactcc tggatgccca tgcggtccga
    10021 gacgtccctg gtcatctcgg tcgagaatgg cccttctcct acccgggtgg ggtacgcttt
    10081 aaataccacg acgacatcgt cgatgcgcgt cggtccaacg ccgttgtctg cggcgatctg
    10141 cgaggccgag gtgtccttgc tggtcacgta tgggtacgtg ccatagtaaa gggaaatccc
    10201 aaagccctgg gtgccttcaa ggatgacatt ctctcctttg tcaagggcag tgttgatctc
    10261 cctcgggaca tcggtgatat aacgtgcgag ttccggcagg tcctttgcct ggcgcgccac
    10321 tctcattacc cggtctgcgt tggcaggtcc gcatccgctc cccgtgctcc cgatggtctt
    10381 tgcaagatga tcgctctgct tgtcccgctg gatgtgggcc tcgtcgatga cagtgcaccg
    10441 cccgtccacg aagaccctgt cccccacgcc aaggacctcc acctcctttg caagcacccg
    10501 ggggtccacg agtactcccg tgcctatcat caggcgggca ttcggataga caaatccaga
    10561 cgggaccatg cgcacgccgt actccttctc ccccatcttc actgtgtggc cggcattcgg
    10621 acccacacct ccacgggaga tgatggtggg atggtcctgg tgggcgatgt gggcaacgat
    10681 cttccccttc ccttcatcgc caaaaaatcc gccgacaatt atggtgcagg tcatggtaca
    10741 accatatact ataggcccgg cacgcggata aaagtatatc gcgaaatctc cggcagggaa
    10801 cgttgctgac ccgcggatat gatcctcagg tcctgacaca cattgcaagt attcacccgg
    10861 aaaacgcgga atgatcacag agatgaaaag ggtcccgtga tcgatcatga tgttccaggg
    10921 gccacagggg agtcccgggc tgatacctgg tatggtatgg taggacagcg aaagtccgcc
    10981 aaggagtatc cagtgcagac gaggggaagg aaaagtgggg gtgacggact gccgcgcttc
    11041 cttcacttca cttgtgaaat gaactgctcc atccggcgga ccgcttcatt gaggtccttt
    11101 ctcgacaccg cataggcgca ccggaggtgg cctgcgcccc cctggccaaa gacactcccg
    11161 gggaccactg ccacgtgctc ctccttgagg agtctttccg cgaactcgta gtccgagagc
    11221 cctgtccctt ccacagacgg gaaggcataa aacgcaccct ccgggaggtg acaggtcagg
    11281 ccgatacggt tgaggctctc tacgaagagg ttccgccgca cgcggtattc cctgaccatg
    11341 cgatccttct cctcctctcc cctccgtatt ccctcgagtg ccgcgacctg ccccatgaca
    11401 ggggcacaga gcatgacgta ttggtggatc ttcagggctg catcgcaaat ctcccttggc
    11461 gcgcagaggt acccgatccg ccaccccgtc atcgcgtagg ccttcgagaa cccgttcagg
    11521 gtgatagtgc gggacgcgag gtcccccacc gtcgcagatg caacatggga tccctcgtag
    11581 gtgagttccg catagacctc gtcacttatc agcaccaggt catggtcgca gacgatatcg
    11641 gcgactgcct ggagatcttc ccttcgcatc accgcgccgg tagggttgtt gggaaagttg
    11701 atgattagtg ccttgctcct gggagtcacc cgttccatca ggcggtcggg attgaggcgg
    11761 aaccggtcct tctccacgca gggaactgcg attggcatgc ctcccgcgag ggtcacgcag
    11821 ggagcgtagg atacgtagca gggctctgcc accagcacct cgtctccggg atcgaccacc
    11881 gcacgaatgg cgatgtccag gccctccgag acaccggtcg tgataatcat ctcattgtcg
    11941 gggtgatagg agagtccgta atgggcatgg aggtaacggg agagcgcttc tcggagtgtc
    12001 tggagccccc tgttcgaggt ataagaggtg ccgccctgct cgattgagta gatgctggcc
    12061 tcgcatatat tccagggagt cgagaagtcc ggctccccca ccccgagcga gatgacctct
    12121 tccatcgtca gggccaaatc gaagaacttc cgtatccctg aagggggtat ctcccgaccc
    12181 ttcttcgaga tatagtctct catccggacc ctcacaggga ataaggtatc cgttcgctcc
    12241 cttctttctc gcagaaggtg cgcccgttct ccttgtatga cttcatgatg atgtgggtgg
    12301 ccgtctcgcg tatgcggtcc atgggggcga tatattcaga gacgaaacgt gcgacctcct
    12361 gcatgttctt gccggtgacg gtaagctgca ggtcatatgc accggtgatc agccggaggg
    12421 atcggacctg cctgaacctg gcaatccgtt ccgcgatgcg atcgtatccg aaatcacgct
    12481 cggggttgac cttcagctcg atgatagcgg atacttcacc gttccctgcc cgctcccagt
    12541 cgatcacggc ggagtagctg cggatcactc ccgccccctc cagtgccgca atgcgtgcct
    12601 cgatctcttc gggtgcgatc tcggccatca cggccatctc gcgggtggtc aggcggctat
    12661 tctcctccag cagctggagc agaagaaggt ccttttcgtc cattatctct tcacctgaac
    12721 agggacctgc accactggac atcccgctcg gcgagtccct gggtactcaa ccgggggtcc
    12781 gcggtgatga cgtcccatat cttatgaata ttggtatcaa accacatctc ttttgtcctc
    12841 ccgtaacgcc cgcggctcac tactcttgta ttgattacgc cgagcatgtt cagctccgat
    12901 atgaggtccg tgatgcgccg gtgagtgagg acatcgagtt ccatcgcagg tgcgatctcc
    12961 ttgtatatcc tgcttacctc gccgctggta aagatggtct gccccatctg gtcgaggagg
    13021 agcatggcat agaggaccac cttgctctgg gtgggaagcg tggcaatgca ttcgatcatc
    13081 gagtcggcct cgatctttgc ctgggcacct ttcacatgcc gctcactcac cctgtctgat
    13141 tcgtcgcggt cagcgatctc gcctgatacc ctgagaaggt caagcgctct ccttgcatcc
    13201 ccatgctcct gggcggcaag tgcggcgcag agcgggataa ccccttcatc aagaaccccg
    13261 tcaaggaacg ccatctccgc acgctgctgg aggatgtcac acagctgcag cgcattatac
    13321 ggaggaaata ccagttcctc ctcgctcagc gaggagagga cacggggatc aagaaaatcc
    13381 ttgaacgaga ggtcgtttga gatccctatc atgctcactt tggacttttt caggtcggaa
    13441 tttatccttg agaggttgta gagggtctcg tcgccgctct tcttcaccag cttgtcgatc
    13501 tcatcaagca ctatgataag gacgcccccc atcgcttcga gctggttctt gagctcctgg
    13561 taaacctggt cggtcggcca cccggtcatc gggatgtgta tccttgcctt atcgctggct
    13621 gtctcgtcct cgccaaggag ggtcttggat atctgggcga ggacccggta ctgggtgtcg
    13681 ataacttcgc aattcaggtg caccacgcgg catatagttc ccatgtgaat tccggcattt
    13741 tcaagctccg aacccacgta gcgaacgctg gcagtcttcc ccgttcctgt ctttccataa
    13801 atgaggatgt tggaaggagt ttcgttccga agggagggtg caagaatgga tgcgacctgg
    13861 tcaatttggg gtctgcggtg ggggagaatc tggggacggt acgaatgccg gaggacttcc
    13921 ctgttcttga atattttatt gctctcgagg tactttttga ataatcccac cgatgcactc
    13981 tggtcctctg ccatccgttc cccctgggag ataaccatat gatcatctcc ctgaaaaagg
    14041 tgcctgtttg gcaaacatat aaaccccttt atttctactc gaaaacaaac tataaacagt
    14101 tgttttcttt attgttggat tttaagtctg ttttttacag aattgaaaca ccaagttttc
    14161 cacaggaacc agcctttcac ggcgagagat ccccgacccc tttgtttcca ctggaacttc
    14221 atgaagaact cctctctttt tgagaggaat taaaagaaga acagctcatt ttcattttct
    14281 tttttgatat ataaaagttc attgaaataa atcgaaatac attgagaaca tcaacaatat
    14341 ttagagacaa gagaaataat tattgagaac accatagtaa cattccatcg ccgttttatc
    14401 acgctaaatt ataaaacagg caataacgca caaacgctcc agtggaaata aaggggtcta
    14461 caaacatgta tgaaatcagc cttatccatc tctctaaaga aaacaggtaa aaaccattca
    14521 aatatatgaa ttgttgaaga tctctatagt ccctgtgaaa aggaaagaca cttactctgt
    14581 gaaaaaagcg aagacttcgg aaaagttgcg agtctcccca ttctgactcc agtggaaata
    14641 aaggggtcca ggttgatggt ataaaataac catatgggta ttgtttattc ttccacgcac
    14701 atatcatatg caacatgaag tctgatcacg aaaggccggt tgccatcaag gctgcagtac
    14761 tcacggtctc cagcacgaga atagaggaaa acgacaccag tgggaaggag attatgaccc
    14821 tcctcgggaa ggctggcatc aaagtcggca attacgccat aataccagac aatatagaag
    14881 ccatacagag agaagtgctc gactcgatca agtcatgcaa ttgtgtgata ctcaatggcg
    14941 ggaccggtct cactcatgat gactgcacca tcgaagctgt ctccccgctc ctcgagaaga
    15001 aaattgacgg gttcggggag ctcttcaggg caaagagtgt caacgaggtt ggaaccgctg
    15061 caatgctctc cagggcaatt gccggcgtct gccatgaaaa agtggtattc tgcattccag
    15121 ggtcgacgcc tgcggcaacc ctggcaaccc gcgagctcat catccctgaa ctccgccata
    15181 tcataagcca tgcaatgaag cgctcctgaa tgtgtattcc ccctcatctc tctcatatat
    15241 ggggaatatc gagggccttt tttattaccg gtagacttcg agcaatgcca cacgttccag
    15301 gacgagctcc tgtatgcgcc ccgggccgac aacctcggcc tcctgcggtg tgatggagaa
    15361 cagttcacag aggtgagcag ccttcgcggg ggtaatcgaa tcccagtctt cgtgatctgc
    15421 aggagagcag aatttcatca gttcgcgcct ggcgctggca ttgtcagggc agatgcagag
    15481 gtacgcggca ttctcacctt tctgcacccc aaatcttgtc ccatgcacga tctggcgtga
    15541 tcccgcagcg tagaggagcg actccacctc cacgcggctg gagatcatgg acccctggtc
    15601 aagtgcccta aatgcatgat gaagggcgga ttctgtgtgg gcctttcccg ccatgttcat
    15661 ggcattgaag agaatgacat gggcatcgtt tcgcctggaa atgtcctgga tccttttgag
    15721 gaaaccctcc agatcatgta tacacatccg aacctggtag ataccgcagc gcatcattgt
    15781 ccatcctcac caaaagagga gagcgttgcc tgacccacgg cagcagacgg tttttcattc
    15841 gtgtcctcat cctccccaag ctcgcgcatc acctgggcgg cgacaccgcg gccgagaatg
    15901 gctgcgacct ttccactctc tgccttcttc agctcttcag gggttgtaat accattgtga
    15961 aagaggctcc ttgcacgcac ccgcccgatg tgcttgagcc ggacgagtgg cagaagctcc
    16021 cgcttcaccc catgtttcat gcatactccg tattccctga catcgcgggc aaagcgcggc
    16081 gcaaacatcc ctgcaagcct gtccgatgca tgcagaagcc agttgacgct ttccaccatg
    16141 ttgaagatgt ccccgggccc gacagagtag cgctcgcaga tggttgcctc ggggacctcc
    16201 attgaccagt cgcggaggag catggcggtc ttgagtgccc ggaaatactc ctcctcctcc
    16261 ccgaacccga gcggctgcca gagttcctcc tcgtgttcaa gcaggaaacg ttcgagatac
    16321 ccgagatcgc ggttgttcac gtagagcgta tacatgtcag gggtgctgca gagagtctgc
    16381 aggagaccca tgtctgaata gtctttcgca gcgcacatgc atctgacgat ggactctgct
    16441 ccccggggat caatgtagag ctgcgagacc aggcctccgt actctgtcgc cgagtacctt
    16501 cccccgtttt cgacaaccat ctccgcagtg tcgagaaacc ggagtgcccg ctcggtgact
    16561 gacccgatca gcctgctccc tttgtgctgg tgcgtgtaga aggtccgtgc catgaactcc
    16621 tggatctctg tgtcgctcct tgcaaatcca gaggcaacca gtgaaagtat gtgggtcgta
    16681 agcgcggatt cgtttgcgca ctgcgagttg acatcctcgg cggctgcatg gatgtagaat
    16741 tcaaagaggc ttgcacgctc ctcctcatcc tttgcgatga gcaccgcctc accgtacggg
    16801 tcgagcctcg gccgcccggc cctgcctgcc atctggtggt actcgcgggc ggggatcgga
    16861 accattccaa cccctgtctc gtagcgacgg tagtcccgta tgactaccct gcgtgcaggc
    16921 aggttcagcc ctgcggcgag tgtcggtgta gaggagatgc accggacgag ccccttccga
    16981 aatccctctt ccacgattgc acgggcatcc ctcggcagcc ccgcatggtg gaacgccgct
    17041 cccatcctga cgcacgaggc aaggctctct ccggtccctg tcgcatctat cttcttcagc
    17101 cggtcccggt acgtgtcaag ttccggatcc tcgcacatga gagcttttgc agcgcgcttc
    17161 gcgaaagcct ctgcattctt ccgggaggag acgaagaaca ggcactgtcc cccttcggcg
    17221 accgtgtcaa ggagaaggtt gatgtcctcg tatttggata cggacactac cgcccgtgat
    17281 tgcccgtcga actgtatcac gccgttcata tagaccccct ggcggaggtc gacaggccgc
    17341 catgtgctgg tgacaagtgc cgcgtcgagc cagccggcaa ggaccctggg attcccgatg
    17401 gttgcagaaa ggccgattat ctggagacgc ggattcatag cccgcatctt ggtgatcacc
    17461 atctcgagcg tgggcccgcg gtcaggggag tccagcaggt gcacctcgtc gacgacaagg
    17521 agagtgattt cggaaagcca gggtgccctg ttccggatca gtgaatcgac cttctcgctc
    17581 gtggcgacga tgatgtcgtt tcgccccagg tactcgtcgc gccggtcaaa atctccggta
    17641 gaaataccta cccgcactcc tttccccccg aattcctcgt atttttcgct tgcgagcgct
    17701 ttcaggggca caatgtagag gctctttccc tctctttttg cgtgcgagtg catggccatc
    17761 tcggcgatga gggtcttgcc gctcgcggtg ggaacagtga caaggaggtt tgtcatctca
    17821 aacaggccct tttcaacgca ttcggcctgt ggaggataaa gatcctctat ccccagggaa
    17881 cggtatctct cttttaagta atgcgggaga ggaagagcag ctaccttcat ttatgacccc
    17941 tttttttcca gtccgcaacg gcctgatcgc gcgcaattcg tgaaaggcag gatgatgaaa
    18001 cggtagttaa ctccaatgga aatcctccct tcccggccat caattgcgcc tccggtattc
    18061 atcgtccaca aggtccattc agtagtaata ttcgagcatg tcctgctttg cgtcctggcg
    18121 tttcttctcc aggtacgaaa agaccgcttc gtgcggggag ccttcgagga gcatgtcgat
    18181 tgccgcacgg gccaccttca tctgttcggg cagcccgatg attgcgatgg tctttcccat
    18241 gacagagatc tgggtatgtg tcatgtgttc gatctgctcc cttgcacggc cgtctctgcc
    18301 tatgatgcgg ccacggagcc ggtccatctg cctgggatta tccgcaaggc cggagagatc
    18361 gatcacctca agcagcaggt cttcgtcccc aagcaaccgg aacgccctgt ccggagaaaa
    18421 acccctgttc accgcctgaa tcacttcgac agcgcggagg aaggggacgg cgtcctctcc
    18481 ctccaccctg acgattccct cctggctgtc aatagcaata accgtcccgg tcttttcttc
    18541 cagttctttc ttgatgagcc cgcctttccc gatcagaacc ccgatccggt ttccggcgac
    18601 tttcagttcc tgcatcatgc gtctcttccc tcatcttcct tgatagtcgt gatctgttcc
    18661 ggttgcgcga tcccggtcac ttcagagaat atctcccgct ctaccctcac ctgacatttt
    18721 gtgccgaaga accggttgat gttggcgatg tcccggacca ggaaatggag cgccctcggg
    18781 tggtccacgg tcaccgcctg ccccatgtcg atgacataca tgcggtcccc gtagaggata
    18841 ttatattccg agaggtcggc atgcaccagc ccggccttct ggtagaggtc tttgataaaa
    18901 ccaatggttt cccggtatat ctccccgggg tcgtcaaggt gcaccgaccg aagctggggg
    18961 tatggacgct cctcctcacc aaggaactcc atgataagga tgttccggtc ccataccagc
    19021 ggctcgggca ccggaagccc tgtctcgtgt gccctcgaga ggttcgcaaa ctctttcctc
    19081 gtccaggtga agacgatatc cttcctggta cggcggattc ctgagaaccg cctgtctccc
    19141 atgatgtact cgctcatcgc attgaaattg gcggtacgaa tgcggtagat cttgatggca
    19201 aggtcccggt tttcccgctc accgaagaag acgttcgcct cttttccagt actgatggag
    19261 cctccaattg cggtgatcca cttcttatgc accagccggt agagcgagag cagcgtaatc
    19321 tcgtcgaaga catcgtccct gaccttcagc tggtccatat cctttatacg gatcccgagc
    19381 tctgcgatct tccggtcgaa cctgtcctcg atgcgatcga tactcattgc tggattccgg
    19441 ctcatccgat atggtccctg acacaggcga ggatatcgga aaggtatgtc gcggtcgtct
    19501 tcttcaggtc gaggggatgg atctctcctt tcctgtatgc ctcttcaatc tcgctgaatg
    19561 actggaatgt gtggtctccg ccgaactttt ccggccttct caccacgacc tcggagaccc
    19621 tcgggaagac gtggtactgc aggatttgga ggacagggtt ctcctcgcac tcaggggggc
    19681 agaacgcttt ctggcatttt ttctgtatct cctcaacgct gtcggcaacg gagatataat
    19741 ttccactgga agaagacatc ttctttccgt caagcccgtt caggatgggg gtatggatgc
    19801 agacaggtga agggtatccg acggacggga ggtgctcccg tgcaagcatg tggatctttc
    19861 gctggtcgat gccgccgacc gccgcatcca ccccgagtcg tgcgatatcc accatctgca
    19921 tgatgggata gatcatctgc gagacggtag gggtctccat ctgccgtccc acctcgtcca
    19981 tgctccgcgt ggccctgttc aatgtgatct gctgggagag gcgaagcacc atcagttcgt
    20041 agtcgcggtc gagctggagg tccgacccca gcacgtactc cacgtcagtg aggccgagcc
    20101 cttcgaagca cctgcggttg tacatggcca gctcccggac ctgctccatg ttccccttgc
    20161 ggttcagaaa ggcgtgcagg tcggcgagga gcaccacgac atcgaagccg gcgtgtttca
    20221 ggtctacgag cttgttgacg gtgaccaggt ggccgaggtg aatctctccg ctcggctcgt
    20281 agccggcata caccctcttc ttcggcctgg cgaggacttc ccggagttcc tcgtccgtga
    20341 ccacctccac ggtgttcctc gttgccagtg cgtacgcgtc cattcatata aaatggggaa
    20401 ttcgtggtgt taatgcttat gaacagcaca gatgcggccg gtcagtaata ctgtgtgggt
    20461 gttgcgcttt tcattgggaa aggtctgatt tttttgatgg tccaggtttc atggcaactc
    20521 actttattct cacaccaggg tctggtgcca tccatgggat gccacaatac ccactcagcg
    20581 gattttcctg gcatctcctg gttttgggag ggctggccag gagaggggag ataagagggc
    20641 tctctttagg catacgctgg aaaacacccg aaaaagaatg tttccagccc aaaaaatcga
    20701 ataggggtta attgattgcc acgaagttca ttgccttgtt ggtcgtggcg atggattttg
    20761 cagggtcttt ctcggtgccc atcattgccc ggatgcagtc gatattctcc accaccacgt
    20821 ccgcttcctg gtggatggcc tggaagagat agagctcctt cccgatgcag gatacagatt
    20881 cctcgaagat gccgttctcc cagaggtccg aacggcaccg ccccaagtcc atcgcgtact
    20941 ccttcagctc ggcggtgctg gtgatcccag tggatttccg gaccagtccc atcctcggat
    21001 gcttctcgat gatctcgagg actttctccc ggctcgcctc ttttttaagg tccatctgca
    21061 cgacgtgcat gtgcatcatt gttacgggga caatcattgc cagcgtgacg atgttgatgt
    21121 ggggcagcac gctctgcacg tccgggccgt ggtggctcgg gatgtgcacg gggttcagca
    21181 cgaccgcatc gattggcccg cgctttatgt cagcagggtc cccgcctctc ctgaccatca
    21241 ccgcccgcac cttctcgacg ccgtaagctt tgtccatggc attgattatc cggcagaggc
    21301 cggtcgtatt gcaggagacc acccgggcga actgcttgcc gatggcgtcc ctgtagttgc
    21361 agtccgagtt aaaggagaac cctgcaacct cgtgatcttc gcctccctgc cagatggcct
    21421 tgatgcccac cttctcgtag agcggcctgt tcttctcccc cacgccgccc ggcgttgcat
    21481 ccaccacaat gtccgcggcc ttcagcatgt catccacgct gcctgcaaca gggaggccgg
    21541 ccttctcgaa tgcagccttc ttcgagagat ctgcgatgta gaggggatac ccccgcgatt
    21601 tcgcaacgaa ggcctcgtgg ctgggcttcg tcttcgagac cccgacgact ttcatgtccg
    21661 gctgggccgc aacagcatcc gcgacccgct ttccaatcgt cccgtacccg ttgatggcga
    21721 ctctgatcat atggtgaggt ggtgggaatg aggggagtta aagattgtca attgtggggg
    21781 aagagaaaaa tcatgactgc gttgcagtca gaaaatgaaa taactttaaa tttggtcata
    21841 tttttcgttt ttaatcaatg aatggagtta tattcttcag aattatttca caagatttat
    21901 gctcttcaat agtgacacac caattaccgg gggagtccgc agaaatcgcc atatgctgtg
    21961 atccagagaa catggcatag tggatgctcc gctgagtgtc tgggcttttg tcttgggctg
    22021 agtagcggat gctgggttgg ttttgtgggg acctttggaa atgtgtatgc aaggtactga
    22081 aattttgtgg agaatgagat atgacaatcg tgaaagtggg aagtaaaaaa gagctttgct
    22141 tctcgtgtat tctgatttca gtcatccttt tcggtatggt aggatgcgtc agcggtgaaa
    22201 catattacat caacagcaca gtgttgaatg ctgaaaatcc ggtgtttata attgaagaac
    22261 ccggaatata cctttttgat gaggatatta gcggaatacc gaccagtcag gatgagtatc
    22321 gaggtgcaat ctggataaaa acctcggatg tcgttctcga tgggcagtat cattcgctct
    22381 cttcagatct cacagatatc ctatggacta attccgctct gataatatct tcttcagaag
    22441 atttcggtga taccccggat cagggttctg atcttgaaaa tgtcacaatc aggaatattt
    22501 acttcgatat gtggcagaac tcggtattcg cctataatac taactcgtta attctgacag
    22561 gtaatgtctt ctcaaacacg caaagcgcaa tttattgtgg caatagtcag gaattaaaaa
    22621 tagaggataa tacattaaat cctggctgta atccatacga ctatatttca atcactgcga
    22681 caagacccag ttcaggagtt ctgattgcca ataattctat ttacggagga gggcctacat
    22741 cgtcaattaa catagactca gaatatgttc aggacgttat tattcacagt aatatcctcc
    22801 agggaggaag tgaaagtgat catcctggaa ttttttcatt ggcggataaa gtgattattg
    22861 aaaataatct ccttcagtgt gaaaggagtt gtgtatttat aagtggtgac gaatatctca
    22921 ttcgaaacaa cacattcgaa tcaaatgaac aattcatcca atttaatgga agaaattcca
    22981 taattgaaga taaccaattc gggtcttccc ccagttacgg ttgtggggta aatattggtg
    23041 acgaatataa ttatgctgaa aacgtctcgt tctcgaaaaa taaggtagat tcggagtttg
    23101 gtggaaattt atatggtaat cgctgtaaaa ttattgactg tgagttctct ggctgggatg
    23161 gaattgcaat aaccggaaac gaaaacatca ttaataactc gattttttac agtaccgggc
    23221 atacaatggt tatatggcta accggtgata attgcgaaat aatcaataac tcgttatatg
    23281 gtggctctcc tggacaaggg ggaagcatca tccagatata tggttcgaat aatattcttt
    23341 ccggaaatac aattcaggga gaagattatg gtataacaat gattgacgga atggcaaatt
    23401 ttatatgcaa taatacgatt tctggcagtc tcaatggcat atcaatatca gattcagaca
    23461 tgaaccaaat atatggaaat ttcataaccg aaaatgagga ggccggcatt cttattacag
    23521 tcatcaacac cgactgccag aatttgatat tcaataacta tttgaaaaac ctgaataaca
    23581 ttgttttttc agctaactcc ccatcatgtt cctggaatat tgaatcgggt atgggtccaa
    23641 acattatcgg cggtcccaga atcggtggca actattgggg agcacctgat ggaacaggag
    23701 tctctgactt agcactggac gaagataatg acgggtttgc agatgaacca tatgagatag
    23761 atgcccagaa tatcgattac tacccgcttg tgcatttcac gtctccccct gaatatggaa
    23821 tctattatct caattctacg gtcctgaaca acgagacaac gcgttatatt attgatcaac
    23881 caggaaccta ccttttcgat gaggatatca tcgatcttga attcccctgt gatacatcag
    23941 gcactttctc cgagggatat ccactcattt gggtacgatc atctgatgtg gttattgagg
    24001 ggaatggcta tgctttagat gcaatcgatc aatccaatgg gattcctgtg agtgcaattt
    24061 ttattgggga ctcaggggat gaaattgaaa gccttagact ggaaaatgtt accgtccgca
    24121 acttatcctt gtcaaattgg tatattggta ttgaatccca ttccgtggca aatcatatca
    24181 ttgagaagaa tttcatcaaa aattgtggtt ttgggattct cattaatggc gagctaccat
    24241 tattctgtga tattagagtg cgaatgaaca cgatcacatc tgatggattt gaggacaatt
    24301 cgggttattg tggaatacag ataaatatgg gtgatttcga tgaaaatatt gtggcgatcg
    24361 tcgaaaataa tcaggtatcc ctattggagg gatgtgccat cagagttgat ggaggtacgg
    24421 cacagattaa tagtaatatt gtgacaaatt gtggagaagg tatctcatct ggctctgctg
    24481 gaattatatc aaacaatatt gtcaccgggt ctaatgattg ggcaattagt ggctttggct
    24541 atgaaatgga aattattaat aacactgtct ctcaaaatcc gggtggcggt ttttacttag
    24601 gccctgctga atattcagta ataagggata atattattga aggaaatggt ctatttggaa
    24661 ttatttttga tggcgctgat tgtcatattt tcaataatta tctgaataat actcaaaatc
    24721 tcgatttaga agcattagga gaatggtgcc cacaacattc ctggaatgct tcagttccaa
    24781 tagaagagca gaacatcgct ggaggaccat ctatcggagg aaactactgg ggaacacctg
    24841 aaggaagtgg atactctcaa actgcagttg accagaacca cgatgggttt gcagatgaac
    24901 cctatgtgat aatttccggt tattgggata tggactatga tgggacacct gaatatcatg
    24961 agaccataga ttatttcccc cttgtcacgt ttgaccaaat tcccgataat caacctccct
    25021 atgcattcct ctcattcgac ttcccggatt cttcaaataa ggacatccct acaccggatg
    25081 atgtaatcac cttcttcgat aattcctggg accccgatga tggcgatacc atcactgaat
    25141 ggtcatggga ctttggcgac ggtgaaacat cagatgaaca gaacccctta caccagtatt
    25201 cccaatcagg gttctatact gtcaatctca aagtcactga tgggaatggt gctatgggct
    25261 catgtgagga ggagatcctt gtcaactacc cgccggttgt ggactttacc atggatattg
    25321 aacctcccct catcgcaggg cagattgttc attttaccgg cactgcaact gatcaggatg
    25381 gcacaattat tgaattgaca tggcaggttt tttccggatc acaaccatat ccagaaatcg
    25441 tgtccaccga catcaacatg tggtatgaat ttacgacccc cgacttatac gtgataaccc
    25501 tcacagccat cgataatctt ggatcaccag gaatagcggg atatattctg gttgtgaatg
    25561 aactggaaga aacactctat ttcctgatgg ctccaggagt ggaattcgat gggataaatg
    25621 tgaccttcga ttctgcatcc gtggatgagt tcattggcaa cgtattctca attgatgatg
    25681 ataccctcac tatcacgatg aacggtctta ccactactct tgttactgac ggctttaccg
    25741 aagtcaatgg cctgcttcaa ggagaagtga attcaataac agtcgaaaat gccccacttg
    25801 tgtgggagat tgcagatatt ggtgatgtgg gggcttcatt taaagcaaat ctgaccccag
    25861 aaaacagcgc gtatctcgag gatgttatca tcgcggtcag tatcaatgaa accgcgagta
    25921 ctggagtcca gaccgcttac gaacttgcag cagtggatgc atcacttgat attgtcgaca
    25981 ttgcctatgc cttcaacatc atcggggcta cgctggaaaa tggcgttgat gtaatggatg
    26041 caaccatcgt catgactgct ccagaaagct gggttgtatc tcaaggtgga ccagaccggg
    26101 tcaggatatt caggtactct gaatatgatg gtacaacaga gattcttgaa ccgatttatg
    26161 tgtttgatga gattgccggg gtgtatacct tcaccgccct ctctccgaac gggctttcgg
    26221 tattcagcct ggtagcggtg atcgagacaa tcccagacac cacaccccct ctcatctccg
    26281 acctcctcgc caccccgaac ccggcttcca taaacacacc ggttcttgta accgttaccg
    26341 ttgatgactc actcaccgga gggtccacca tcacgaatgt agaatattca gtggatggaa
    26401 tcacatggaa ccccctgatc gctacagacg gagcctttga cgaaatgaca gaaagtgcaa
    26461 ctgtcacact tccgggaagg gcctctgccg aagtgttcaa tgtccttgtc cggacaattg
    26521 acgcggcggg aaataatgca ctctccgacc cactcctcgt cgcagtctac gacccgaacg
    26581 gcgcgttcgt cacagcagga ggatggatcc tctcaccggt gggagcatac atgccggatg
    26641 caacatcaac aggaaaagca aaccttgggc tgaactgcaa gtacaagaag ggaaccacaa
    26701 tccccaccgg ggagaccgag ttccacctaa aggatgccga tctcaagttc aaggcaacca
    26761 gttatgaatg gctggtaatc acggggccaa aggcaatcta tcgcggttct ggaaccatca
    26821 acgggaatgg tctctatgga ttccagattg tcgcagttga tggcgcgctc tctggtggag
    26881 acgatacttt caggatacag atatgggaga aggctacgaa tacgctcgtc tatgacaacg
    26941 gcgcaggatc aacgactggg gacccgggca ctacgctggg tggcgggaac gtgaagatcc
    27001 acaaataact tttttctctt tctctcgccg attttaatcc cataatatgc ccctctttct
    27061 ctcccctgtt ccgccctacg acctcatcct gagttgcagg gtattcgctg aaggcgaccc
    27121 tgcaatacgc cggttccagc acggggtcct ccggcaggtc atcagggtag acgcgggacc
    27181 agtgctggtg caggtgcggg acattggtga tggagagaat ccggctcttg ccgttgacat
    27241 cctctctgat gacgaagtcg gcgaaaatgg gagggagaga gcaggaaacc aggtctcact
    27301 tcaactcagc cttcaggacg acctgcggca gttctaccgt gcgatccgcg gggatcccct
    27361 gatgactggg atcgcacagt ccctgtatgg cctcaaggcg ccgcaatcgg ccacggtatt
    27421 cgaggccctt gtcacatcca tcatcgagca gcagatctcc acccgggttg caaggattgc
    27481 cgaggccagg atggtaaggc gcttcgggga gcagatggag gttgggggaa tgacgtattt
    27541 ctcctatccg gaaccttcgg accttgcagg gggcaccatc ggagacttcc gggcatgcgg
    27601 tctttcggca aggaaagggg agtatatcaa aaacgcggca gagatggtgg cgggaggaac
    27661 gctggatctt gagtccatga aaaatattcg cgattcgagc cggatcatcg aggaactgtg
    27721 tgaactgcgt ggcgttggga ggtggacggc agagttcgtg ctcctgcggg gcatgcacag
    27781 gatcgacacc atcccggcag atgatcttgg ggtcaggaga gcgactgcca gggtatactt
    27841 cccgggggag aggatttcgg ccgaagaggt gcgggccatc gcggaatgct ggggtgaatg
    27901 gaaaggcctt gccgcatact atctccttgc cagtgaacac gattgagaag atccggagaa
    27961 tacatgaatc cagaaagaga ctaagagaat gaaaaccagt gtaaacacct aaaaacagtc
    28021 gtgaataaga ggaacacccc tcacatgtca gcaaggaaag aaccagggag atggggtcga
    28081 cccgatgcat acgtcatcta ttcagaaaga atgtgaagag ctcgcagatt tcgcgacagg
    28141 tggcgaactg gggaggggta ttgcccgact cgtgcttgcc tgcagtcccc gggacctgca
    28201 gcggatgaga tggaacttct caaagaagat acgggatatc agccccgtct accggaaagc
    28261 tctggaggag acgatcacag gatatctcca cgggacctac cagaacgtga ggttgatgaa
    28321 ccagcagggg gtgttctcgt cgatgaacga gaggctgccg gcacaccacc cggcttactg
    28381 gaggatggta tccgcacact gcgcgacagg gaaccccgag gagaaccgga tccggtttct
    28441 caagtacctt ttgagcgggt tctgcatgtt cgtacagggc attccgggcc atccggtcgg
    28501 aatgccgttt cccggcggag acagggtcga gagcatagac ggggtgtatt actgccctgt
    28561 caggacgaaa gcaaatgacg tggatgcggc cctctgtccc ttctgccctg cgctccagac
    28621 cccagagtca gggtatctca agccaccggt gaatgcaagc gagcaccgaa agcaggaatt
    28681 tctcaggaat tgttttgacc gtcaccatta caatggatga aaagaccgga tccaggcttg
    28741 gtgcatgaaa gtgatgcagt gttggaatac acctcataag tgatttcacg atatagtgcc
    28801 aggaatgatt gagatggcgc tgatggatca gctggcgcgc ccaggcccgg gtcaacgatc
    28861 acattttttt aatcagaaat gagagaaaca aacaggagtg tgcaggcttc aatggcagac
    28921 gaagcaagag aaagggctga aaaacgactc ggtgaagtga agggattcta ctcacatctc
    28981 gggatcttca ttttcatcaa tgtcctgctg ttcctcgtga atatcctcac ctccccggga
    29041 caatggtggt tctactgggt gaccatcttc tggggaatcg cggtggtatt ccacggatca
    29101 cgcgtgtttg ggaaaggaag ggtcttctcg agcgaatggg aggagaagaa gatccggcag
    29161 tacatggaag aggaagcaca aaagaagtag cagacacgat cttttccggc aagccggtga
    29221 aggagagaga tgcccggcga gatgcatgag gggcgcgggg agaaaatacc cgtcctttgc
    29281 tcttattttt cgtccttgac agggggctgg ttgatcatcc tccagtaaag tcccatctcg
    29341 cggatgacat ttgcaaactc atcgaactgt accggtttca cgatgtaact gtttactcca
    29401 aggtcatagc tctgcacaat atcccgttcc tcacgggagg aggtcagtac cacgacaggg
    29461 atggtgcggg tcttctcgtc ttctttcagg cgcctcaaaa cctcgattcc atctaccttc
    29521 gggagtttga ggtcgaggag gatgacacgg ggctggcccc tgatgtcccg tcccctgtag
    29581 gggcccgttc cgaatacata gtcaagcgcc tcttctccat tccaggcaac atggacccgg
    29641 tctgagagat tgcaccactg gaagacgtgg aggataagct ccacgtcgtt tgggttgtct
    29701 tcgacgagga gaatctcagc aggctttccc gtgtgctcca tgtgcacatg tgacacactg
    29761 ggagagaaaa aggttgtggg ataaagcgcg tctgaaattc atgcagatat tagaggcatt
    29821 cccggcattg ctgtcatgta ttgcatttta ttccgaaaag aagtccaatt acctgatttt
    29881 tattgattgg caaatattta ttggggggtg agggggaaca caacactgta atgatcctgg
    29941 accaggagaa gatcacgagg ataaagaggc ttctcaaaag ccgtccgaaa gggctgacaa
    30001 tctcggacat ctcgcagatg ctcaagctca accggaattc agttgcaaag taccttgaaa
    30061 tccttctcat caccgggcag gtggagatgc ggctgtatgg caatgccaag gtctactccc
    30121 tctcccagcg ggtccctatc tcctctatgc tgaagtttgc aaccgagttg atactgatcc
    30181 tggacacgga acaaaagatc atcgatgtga atgacaattt cctctccttc ttctccctgc
    30241 aaagggacac acttctcgga aatgatatca gggcggccca gattcctgga atttcaggta
    30301 ccatccttgg tgaattgctg acagattccg ggcggatgga tgaagggaag caggagatca
    30361 gtttcaccaa ggatggtcgg gagtatttcc ttgccctgaa ggttgtccca accgtctttg
    30421 acgacggaag caggggattc accctgatat ttgaggatgt gactcttcca cgttcatatg
    30481 aagagaaact gaggatcaac gaggccaggt acagggcaat tgttgaggac cagactgagc
    30541 ttatctgccg gttcaaaccg gacttccggt tcacgttcgt gaacgatgca atatgccaat
    30601 atgtaagacg ccaaaaggag gaaatcatcg gcacggatat attctcgcaa gtctatgagc
    30661 acgaccggga cgtggtgaag aagaatatcc tctcgctgga ccatgagcac ccgttccttt
    30721 cccatgaaca gcgtgttctc gatcctgcag ggaacatccg atggcagcag tggacgaaca
    30781 gggcaatctt caacgattca ggagagctcg tggagtatca gggagtggga agggacatta
    30841 ccgagaggaa acatgcagag gagaacctcc tcgtcatgaa catggcgatt gcctcctcca
    30901 tcaacgggat tggactcgcc acgctcgatg gcaggatcac ctacgcaaat gcctccttcc
    30961 tgaagatatt cggaataccc agcctggaag atatcctcgg atgccacata gaaacactcg
    31021 gaaatcctct caatcccggg cccggagtaa aagacgtgct aaaggtcttg agagagggag
    31081 gcatatggaa aggaaagatc cttgtcatca ggaaggatgg gagccgggta catacccacc
    31141 ataccgccaa cctgatcagg gacaagaatg gcacacccat cgcgatgatg tcctcgttta
    31201 tcgacatgac cgagaaagtg gaggccgaaa aagagattca aatcaagaac accgcgatcg
    31261 aatcggcaat caatggcatc atcatcttcg atgccgacga acatctcatc tacgcgaacc
    31321 aagcgttcct gagcattttt ggcgcaaaaa gcctggatga gatgcttcag aatcacattg
    31381 atgaattctg gaagattgca agagagactg ttccccccgt tgatgacgtg aaaaaaatgc
    31441 tcattgcaga agggaggtgg agcggggagc tgcgggtgaa gatgcctgga gggatgctgc
    31501 ggttcttcca ggtctcggca acgcaggtaa aggacgaaag gggagcgatc ctttgtggga
    31561 tgggcacctt catggatatt acggatcaga aagtgatcga aaaagccctc aagaccaccc
    31621 acgagaagct gcaggacgcc atcgagttta tgccggaccc tacattcatc ctcaaccgcg
    31681 aaagacgggt cattgcatgg aaccgggcgc tcgagactat ctcgggtgtc aagagcagcg
    31741 acgtcctcgg ttctgattcg taccagttcg cattcgggtt tcttgggggg acccggccta
    31801 tcctcgtcga catcctcgac ctccccgcac acgaactggc aaagtcgtac ccgggcatca
    31861 ggaggtttgg ggatagcatc tttgtcgagt ccttgatccc aggcagccat gacgagaaag
    31921 ggatatatct ctgggggaaa gcaacccccc tctttgaccg ggacggcaac atcatcggcg
    31981 cgatcgagtc attccgtgac atgtcggagt ggaaaaaagc cagggagacc atgcaccccc
    32041 acccggaagt ggaaggttgg acacccggct cttccgggga atccagggga ggagagcgcg
    32101 agagccttgc atgcctggag attgcctttg cgcacctcag ggagggcgtg gccatccttg
    32161 acagaaaagc ccgtatcaga atggtgaact ccccgtttct cgcactggca aagagcagtg
    32221 agaacgtgct gatcggaaaa gaactctccc agttctgcca tcccgatgac aacccactta
    32281 tcagggagta tctggattat tcagggatgg atggggatgg atcacccact gtcaggatac
    32341 gggtagggga aagggatgat ctatttgacg cggaaatatc acccatcagg ggagcaggag
    32401 gggtgcttct cgggcatctt gttgtcacga tccctacctt tgctgcgacg aatcagatgt
    32461 agaagccctg gatctcgaga atttccctgc tgtcctttgc accggcatag agtccaagag
    32521 gttcttcatt cagctcgagg aaccggattc cccacggcgc ggtccctagg atgaggacag
    32581 cctggtcctg ctcgccgagg atgaagaggg cagcagcgag cgcctccacc gaagagagcc
    32641 gccacggcct cccgaagttt acggggtttg cagccacgag gaaggggaga gctctttgcc
    32701 tcctccagga acccgacggg atcttatcga gcacctccca agaacagtcg agcgccgtga
    32761 ttgagcggac cgggcggtca gccggagaga gcgcctgctc ggccctcggg tccaggagaa
    32821 gggtgttcct gggaatcctg gagatcttcg agagcacccg cacgagtccg gagcgttcca
    32881 tccgtttgat ggtgcacttt cgcgggtcgc aggaattgtc tctgaatgca tacaggggaa
    32941 tcatctatct cctcttcgcc tctaccactc tatcaatgta cgtgttgatg tgcccatgta
    33001 tcctgcaccc cgaactccgg gcacgcggga tcacaagggg cacggatctc caggcgtttg
    33061 gaaaggcaat cgagcgctgc cggatgttcg aaatcgagat cgttcctctt ccgtgcccgg
    33121 aaacccttta cctcggaagt gatcgcgacc ccggcacatt tctcgaccgg ctcaacacaa
    33181 ccgagttctc acgcctcatc gaccagcttg aagaagaggt gcggaaggta atcacgacac
    33241 gtggccggcc cctttgctgc atcggggtca actcctctcc tacatgcggg gtcgacacca
    33301 cctattatgg cgcagaccat gcgggtccgt cacggcgtcc cggaaaaggc gtgttcctgg
    33361 accggttccc cgatcttccc atgatcgatg tcctcacatt cgcctgcttc cggatctacc
    33421 ttgccgcacc tctcttttca acggcagaga ggcgttttaa tgcctggctc tccgaaatgc
    33481 ttgccgatca cctcttctca gtgtaccttc cgcaggaagc aggtgaggag gggtgctgcc
    33541 gttccgagga ggcccagcgg gagatgtttt cacgccattg cgaggcgctc tccagttcgg
    33601 acgtggtggt tgcagtcatc gacggtgcgg atgccgattc cgggacctcc tgggagatgg
    33661 ggtatgccta tgccagcggc accccggtca tatctgtcag gacggatttc aggagggcgg
    33721 gggcacatga gcacgtgaac cttatgctcg agcagtcatc cagggtggtg cgaaacgagg
    33781 acgagcttct cagggcgctg cagtcaccgt ttctggttga acaccggccg ttttagtcct
    33841 gctttagata actgcaaccc aatgtggaga cacagttgga attctggata tgaaagatgt
    33901 tattgagatt cgtctaataa agacactcat caggtaagca gataattcaa tataaccggt
    33961 gaaaaaaacc cttgaaacgc tcagaattca ttcgatgaaa accagatata tttatcaata
    34021 ttttggatcg agtattcatg caatgacaag tcaccggaaa agcaggggac atccctctcc
    34081 agggattcct ccatgtgatt tgaattcagg aaaaatgatt gaggattccc gcaagcatgc
    34141 gtggaatctc gattctattc aggcgagcat acggtcagac agatatatcc agatccttct
    34201 cttactcacg gctcttggtc tgttattgag attgtataac cttggattta attcgatttg
    34261 gttggacgag gctgtgacgt ataactcttc aaagcttccc ttcggagttc tatgggaaag
    34321 cataaaaacc ggcagtgatt tcaatccccc gcttttttac atgatcgaat cattgatgct
    34381 ttcctttgga aaaaatgaga tcactcttag gctgattccc gcgatagcgg ggattataac
    34441 aatccctgtc ttttattatc ttggaaagga attttccgac agaaattgcg gcattatctg
    34501 tgcagcactt gtcactttct caccattcct gattttttat tcccaggaag cacgggcata
    34561 ctcattactc ctgttgctga tcgctctttc cattttgtgt ttttttaagg gaatgaaaaa
    34621 tggcagaaca cttcaatggg ttttatttgg acttttttct ggtatcgcgt tctggaccca
    34681 tttttattct tcaatcgtat ttggagtcct gataggattg ggtcttctcg gaacaggcat
    34741 ctccaaagag aacagattgg ggaaaatcaa ggctatcatc tactcgctga tcacattcgg
    34801 ccttgtgacc ctgccgcttc tgatagtgac ggcggaatta tatttggtga ggacggcatc
    34861 accccccact ttcggaattc agggattgaa tgtaatatcc catgccttta cagatctttc
    34921 aggatttaac ataattccaa ccgctttatt catctcactc tttgggatcg ggcttctctg
    34981 ggcattcctg catgaaaaga tgaagggagt ctcaatcgca ctcccggtga tcgcaatact
    35041 gctgataagc tattatctct cgtaccgcat gccatttcaa acacggttcc tcataataat
    35101 gatacctttc tacttcctgg gaattgcgta ttcttcacgg gcattctgtc aaatattcag
    35161 gaaaaatatt gttgtatatg cgataatcgc ctgcattttc ctggtaaacg tcccgatact
    35221 tacagagtat tacactgtcc agacaaagga aaactggcgt gatttcgcga atgaatttga
    35281 acaattcaca acggatggcg atgtggttgt tctccttccg tcatacatgc aacaaccttt
    35341 ggatttttat tatgattcaa ctcgtgacca tacgaaggaa ttaggcgctt caaacagcgg
    35401 agatcttgag aagtttatgg aggagcatgc cgggaaacgt atatttgtaa ttatgacagg
    35461 agacattctc gcagtggaac cctccggaga cactgtcggg tggttaaagg agaatgccac
    35521 atttctgggt caatatacag gaatttattt gtttcaaatt tcagagtgaa aaaattggat
    35581 attaaaagcc tggacatgaa cgagggtaat gatatggaaa tgatggataa cgatattttt
    35641 ttggatttta aaccatgaac cttcggcggc ttatctattt tactcttgcc gcagtgggaa
    35701 ttataatatt aataattttt atttatagat caggcttata ccagaaccct ggaatcctcc
    35761 tttccatcaa catccccgtc cttcttttgg cgttcctatt cactcaaatg acaatctttc
    35821 tgaagatcaa acgctggcag tatctctgtg ttcaatataa tttccccata agtcttcgtg
    35881 aatcaaccga agtcgtaata gctagttttt tcccagcagg tgtctcccct gggagaatag
    35941 gggatttcat gaaggccgac ttcatgaaaa taaaatattc tgttcctttc agagatggaa
    36001 tctcgatggt attttacgaa cggatatttg aactcattat aatattttta gttgcagttg
    36061 ggattctctt cattggattt tcggctaaat actatattat attggaattc acgttatttc
    36121 ttcttatttt tcttggagtt atgtatcttt tttcagatag atttatccat tttttccaga
    36181 atattgccag aaaaaaattc aggattttca atagtgacga gataaaaatc aggaaaattt
    36241 ccccgaggat cgcattcaac gtatctttat atacgattct tgcattgggc tgtgaattca
    36301 ttaggttgtg gcttgttgca tattcttttg gatattcttt acagatcatt catctttcaa
    36361 ttttcttttc gttggcggtt ctgataggtt tactgtcgca aattccaatt gggatcggga
    36421 taacagaagg gtctctctcg ttttttttaa tggaaatggg agtgccatat gaaattgcag
    36481 ttggaattgc agttattgac agagcaattt cgatgtattt cgtaattctc ataggttttt
    36541 tttattatca ccgaatgcgc acgtattacg tgaatgaagg ggaaagcgtc aataatcatt
    36601 cccgtattca atgaagaaga tattttagcg gaataccctg tgaatctgtt ccctgtcctg
    36661 gaggacattt ttattaaata ttctatggat ttcgaagtca ttcttgttga tgatggaagt
    36721 acagatcgca gttggttgaa aatatctgaa ttacaaaggt tgaaattata tcttaagacg
    36781 tataaaatgc cgcaaaatgt gggtatggga ggtgctgtgc gtaaaggaat tgaattgagc
    36841 gaaggtgaaa tcatcatcac gctggatgcg gatcttactt ttaaaccaaa agaaattgat
    36901 attcttctta ccgaatatgt gaaaaacccg gtggattgtg tttcaggatc cccatatctg
    36961 aaggacgacc tcatgttgga tgtcccttta gtgagaaaaa ttctctcaaa agcggtcaat
    37021 tatatatatt gcctggagct tggccaacgt attaccagtg tcagtcctat tttcagactt
    37081 taccgaagtg aaatattctc gacaattcct cttacaagta ataattttga ggtcaatgca
    37141 gaaattctct cgaaaatgat actatccgga tatactgtaa gggaagtccc tgcacacctt
    37201 tttaaacgtg agcacgggca atcaaaagcg aaaatttgga aaagcatccg aaatcattca
    37261 gttatcattg gaaaaatctt taaagtgaag tttttaaaag gaaattggaa ttgactggtt
    37321 aaaattaaac aagggatagg atgaaaaaca atacgatacc aatagaatct ctatcagatg
    37381 aggatcttta tcgcttgaat atttaccatc gcacagatga tgaacactct tttaaacagc
    37441 tacattcact ctatggacgt gtaaatgcag cccaagatgc tacgataatc aatcaagtat
    37501 atggaaggag agtattggat gtaggtgcag gatatggaag cctatcgagg agattactcg
    37561 attctgcatt tgaggtctat tccatagaac caaataaaga cgtgagagag cttgcattgg
    37621 cctggcacaa cgtgaaggaa ttaccttata gtatttacga tactccattc agcagtcatt
    37681 tttttgatac tgtcattctc cgcgaatgcg ttgaacatct tgacatgccc agagcgatga
    37741 aagagatctc ccgtatatgt agaatgcgcc tcttgatatt tcaaacaaat ttaaaccctt
    37801 taatttcatt attgaggggt atattagggc atcacgaata caatcctcag aaccttgcgt
    37861 attatcgtaa ttgtgcaaac ctcgcaggtt tctcggttca gaatgtttta ttcagggata
    37921 tcttggcgtt cccactttca gggggatatg tatctcattc agtgatcccc cggcaaccgt
    37981 tcattgagca ttcaataatt cagattgatg agaaattatc ccgctttttc aggttttttg
    38041 ggatggattc aatcgtctct tggaggtttt tattgattgc tgataaagcc attttgtatc
    38101 gcagagagca tgaaaaagta aaaactcaat agaaccataa tacaccacat gcccaacctc
    38161 accgtcgcag tcatcggccc ggaaggctat gccggccatc tgggaaagaa aggaaccgtc
    38221 tccgatatca cgttctacaa ccagaagagg gacgagacca ccgtcaccta tatcgagccg
    38281 agccgttacc cggaaaagct ctcctcgctc ttcttctcca tctcgatggc gggaatggct
    38341 ctcctcgtcg tcgacgagat caatgcggcg ttcggggagt gcgtcctgat gctggacatg
    38401 gcgaaggtga gaaagggagt ccttgtactt cggaattacc tttcccccga acaggtagcc
    38461 ccgctcatca agggcacagt ggtcgagcat ttccaggtcc attccgatga ccacgggcag
    38521 ctgagggagt tgttcctcga agaggccgcg aacgcatcgc aaccggccga ggggtcgaag
    38581 ggaggatccg tccctgtcga tcatcacttc ccggtaaagg gggtagggat cgtggtcctg
    38641 ggtggtgttg cggagggaac tatccatgcg cacgaccaga tgaaggtgct ccccaccggg
    38701 aaggttgccc acctccgctc catccagaag cacgacgacg atgtctccga ggcgtcggca
    38761 ggggaccggg tgggtcttgc gctgaagggg gtggaggaag tcgaccttga ccgcggatac
    38821 gtcctctcga ccaatccctc cctcgtgtgc tcaagtatcc tgacggggag ggccgagctg
    38881 gtccggtact ggcctgcgcc cctgaaagag cagatgatcg tataccttgg gcactggatg
    38941 cagttcatcc ctgccaggat cgcattcgtg aacaattccg gagattggaa gagacccgag
    39001 atcacgctcc ggtcagagaa agaactcgtg tatcctccgg gagcaccgat cctcatccat
    39061 tacctggagg ggggaaagct ccgggtggta ggaaggatcg tgccggcatg agcgcggtgt
    39121 agttggcgta accacccgga atttgcagag cggaggagca ctcttgcggc gttccgggac
    39181 gtggacttaa atagatatgc gggagatagg tgaagttatg gcacttgggg atctcatacc
    39241 ttggatcgca gggggcatca tcctcctcgt aattattgga ctggtgctct attttgtcgg
    39301 gatatacaac cggttcttca gcctgaagaa ctcgtctgaa gcgacactcg gccagatacg
    39361 ggtggccatg aagaaacgac tcgacatgat cgaccagctc ctgggtgcgg tcaagagcta
    39421 cgccgctttc gagcgcgaga ccttcgagaa ggtcactgcc atgcgggcga gcgtggggag
    39481 cgccggcccc ggcgacctca accagatcga acgggaatcg cgctccctcc tcgggcgcct
    39541 ctttgccgtc gcggagaatt accctgacct gaagacgaac gccacggtga tgaacctgat
    39601 ggacgcggtc cggggtgtcg aggacgagat cgcccgccag aggtatacct acaacaacat
    39661 ctcccagcag tacaacaccc tccgcgacgt gataccctcg aatatcgttg ccaggatcct
    39721 tggactgtcc aagctcgagt acctggagtt tgaagaggcc attcagacac cgcccaagat
    39781 cgcgttctga acggagcagg tcatgggaga gacgagagaa ctcctgctcc tgatcgtgct
    39841 cggcttcgtg ctgggtgcag ctgcagtact cgtatcactg gccatccccg tactcctgga
    39901 gggagacctg gtcgttgacc agtacagggc cacgctccat gaggacggca cgttccagga
    39961 gttctatacg tacgaggtga agagcagcgg gcagtaccga atgctgtacc gatactggca
    40021 ggacccgctc tcgtcccagc cgctcgactc gccgtacatc tggttcgtgg agatggacta
    40081 tccggcaggc aacgtgggat acatgaaaga ctgggccggg aaggtcacac tcttcggtga
    40141 tgataatcca ctttacatcc ggtttgtcga ggaccgcgcc gagagaaacg aggtcgggat
    40201 ctacaacccc ggatatttcc cagccggaaa gtatactgtc tcgtatacgt atgttctcca
    40261 tcctcctatc gaatatgaca aaattgacgc tcacctgaac cttcgccttg tggacgagca
    40321 tgtcccgttc cgaacagtcg agatccgaat ccccgaacgg tatgccggcg aagtcttccc
    40381 ccatccgcca ggccttgcag tcacgcgtga gggcggaggg gtgttgatca aggggagtgt
    40441 tgcagcagac gagacccttg gttttgagct gctctttccg gctgctgaag cagcgggcat
    40501 cccgggtttt tccagttacg ttgaggatgt gaggggaaag acccgggccg caaactccat
    40561 catccaacaa atccccctcc tgggtgcatg ggcactctac ggttccgggg tcgtgatgat
    40621 attcctcacc ccgctccttc tcctcctcat tcacaggagg ttcggccggg agaaagagtt
    40681 cacagttcca gagtttttga gttttacccc caacccggcg atgaagccct gggcagtgaa
    40741 cctcctcttc aagggtgatg cgatcgagtt cgaccaggac gggtattatg ccaccctcct
    40801 cgaccttcat agaaggcgcg tgatcaccat cagagagaag actgacggaa aaggcattac
    40861 catcaacgtg aacgagtggc agtcggagga gccatacgag cagcgggtgc tggactttct
    40921 ccgcaacgtc gaggaggagg ggatcatcga ctcggactcc ctccagaaga tggcggtcct
    40981 ggcaagaagt gacagggagg tggagaagag gatgcttgga taccagcagc agctctcggg
    41041 ggttacgaaa aagtcagatc cccgcctcat ctcccagtat atcgtcgacg ggagggacca
    41101 cctcttccct ctcctcttcg tcggcatcgc gttctgtgct gtctcagtga tggtgctcat
    41161 cgtggttccc gttctcgcag ccattcttgg acccgcagtt gtcctttggg ccggggtgat
    41221 cgtccagtcc ggggtcgccc ttgcattccc atccacgctt tttggtcact ggaaagacga
    41281 ccggtacaag gagaagctgg agtgggattc gtttgcccgt ttcctctcag acctggccct
    41341 gatcaagcag tacagccccc aggacatctc gatgtggggt gaatggcttg tctatgggac
    41401 ggcgctcggg gtgggtgaga aggtggagaa ggcaatggag actctccaca tcagcctgcc
    41461 agaggctgga gttccgctgg cgatggggct ccggaccgcg tttatccccg tcctcgcatt
    41521 ctcagcgcca tcgcacggag gaagcggggg agggttcgga ggcggaggat cttttggggg
    41581 aggcggagga ttcggaggcg ggggagtggg tggccgatga agtatggggc gtctgtatgg
    41641 ggaggtcttc atggactgca ctgaaggacg cattggccgg gtctttgtcc tccgcatcga
    41701 tcacggagaa gacatccttg agacactttt gcaatgcatc cgggagaagg agattttctg
    41761 tgggactgtc caggtgctcg gggcactcgg gaaggggcag atggtgacag ggtcagaaga
    41821 accggtcctc cctcccctcc cacactggga gaaggttgat ggggggtggg atatcttcgg
    41881 ggtaggcact gtctcatggg gagaggacgg cccgcacctc caccttcact cagcggtggg
    41941 acgaggaacg cgttcactgg cagggtgcct ccgcgaacag gcgcgagtct acatcgtggt
    42001 ggaggcggtc attcttgaga tcaatggcat tcatacggtc cggactcacg acgaaacaac
    42061 cgggctcacc cttccccggt ttctcccggg gaatccccca tgaagaggag acagccgaag
    42121 ttcctgccga tgagcaaggg ggagggaaag ggcctccgaa tcgaccagtt cgacgtgatc
    42181 cttgtcagtg gtgacgccta cgtagaccat ccctcattct caaccgcgat catcggcagg
    42241 gtgctctggg acgccgggtt cactgtgggg gtgatcgccc agccggactg gaagggcaac
    42301 gatgatttcc tgcggctcgg ggacccccgc ctattctttg ctatctgccc cggcaactgt
    42361 gactctctcg tgaacaatta cacggctgca aagaaaaggc gctcaagcga cgtctactcc
    42421 cccgggggca taccccggag gccggatagg gcagggatcg tctatacgga cagggtccat
    42481 gcactattcc ccggaattcc tatcgtgctg ggcgggatcg aagcgtccct gcggagattt
    42541 gcccactacg attactggtc cgactctgtc cgtcactcca tcctcgcgga tgcacccgca
    42601 gacctcctgg tgtacggaat gggagagcag caggtcaggg agattgccct tcgcctcgat
    42661 aagggagaac cccccgtatc cctgaccgat atccgtggaa cagtctaccg ggtcccgccc
    42721 gcgcagtacg atggcacatg cggattcccc gctctcgaga tacctccatt ctccgaagtg
    42781 gcggccgaca aagtcgccta tgcccgggca tttgccacgc attaccgcga gcagaaccct
    42841 ctcaggggga gggcggtcgt gcagaggcac ccgaagacca tcatcatcca gaacccgcct
    42901 gcgctccctc tctctacgca ggatctggat gccatctatg agctgccata ttcccgggag
    42961 gtccacccgt cgtatacgct ccctgtcccg gcgctggaac cggtcaggtt ctcgctcacc
    43021 agccatcgcg ggtgctttgg aggctgttct ttttgcgccc tcacccatca ccaggggcgc
    43081 ataatccaga gcaggagcat cgattcgctc gtaaaagagg caatccgcct gacaaggatg
    43141 cggggttttt cagggattat cagcgatgtc ggcgggccga ccgcgaacat gtacgggatg
    43201 gagtgcgccc gctggcagac ggaaggaccc tgccctgaca gggcctgcag cgtcgggtgc
    43261 ccgaacctcc tggcctctca ccggaggcag atggcacttc tcaggcgctt aagagaagtg
    43321 ccgggtgtga gaagggtctt catcgggtcc ggcatgcggt acgacctcat cgtggcagac
    43381 cccgaagatc cccttcccga gatctgcgcc caccacatat cgggacacct gaaggtcgcc
    43441 cccgagcaca cctcccctgc agtcaccgcg ttgatgggaa agccgggaaa ggaagtcttc
    43501 gagcggttcc ttgcatcatt cagcaaatgc caggaaggaa aggagaaaaa gcagtacatt
    43561 ctcccttact tcatgtccag ccatcccggg tgtactgtcg gggacatggt cgacctcgca
    43621 gtattcatcc gcgaccacga cctctacacc gagcaggtgc aggatttcac gcccaccccg
    43681 atgacggtgt cgacctgcat gtactatacc gggctcaatc ccctcaccct cgatcctgtc
    43741 catgtgccga aaggacatga gaaacaggtc cagcgggccc ttctccggta ccgggacagg
    43801 catgtcaggg gcctggtaga agaggggctt tgctttgccg ggagaaaaga tctcctgcag
    43861 aaagggaggg ggggcctcct ttcttcgcgc gaaaaaaagg gaaagtcagg gatgaggcag
    43921 gaggagtgtt agcgttccgg gatatcgatc cccctgttct cgcaggggag gcaccgggac
    43981 atcttctcgt cgaatatgcg gtacgcgttt gccagcaccg aagagtttga gatcatccct
    44041 gcaatcagga tcacttccag gatctcctcc ctggtagctc ctttcttcgc cgcagcctgc
    44101 atgtggtagc tggtacagtg cggacagcgc agagccgcac cgaccgccat gctgataagt
    44161 tctgtatact tggggtcgat ggcactggtc tttatcaccg cggtcttgta gagtaggtgg
    44221 gatatgagca cctcgggccg ttctgccatc cgtttgaaga tgaggggtgc acggccgtac
    44281 tccacctcga tatccttcat ccatgcctct gcgatctctt ctccaccccg ggatacgatc
    44341 tcctgaaggg tgagttcgaa ctcctcaagg tcgttcatgt gcagccttcc agtctacata
    44401 tcgtcctgca ggactcatta tactgcctgc aagggcccga taggtgtgaa aaaattcgca
    44461 gtgaaagtca tgtttttatc cgagttgcgc caaagatctg gatggttccc agacaattga
    44521 ggaccaggag agatatatat ggtaaactgg agtccttttc tacactccac atgcctggtg
    44581 ctgctcgtcg cgctttgttt cggagcgata ggagcggtcc aggctgcacc gatgagatac
    44641 gatcttaccg aaaaattttc atcgcaccgg atgcaggata acagtcctgg caagatgccg
    44701 ggaggaaaca tgtttgcacc ggttacgtat tcccgcgctg atattgccag tgcagccgca
    44761 ccctggaaga gagatcactc ttccccgggc attgtcatgc gcgaaagcgg catgggtgag
    44821 acacttccga ttgtgtcggg cacccagaag ttcaacttca ccatccctga acggaacacg
    44881 acgacggttc ctggcaggaa catctcattc aaccgcccgg tcgggaaagc atccctcgtg
    44941 tcaggcaccg ggaccgtggt ctacatggat ctcgagggcg gcttctatgg catcatcgcc
    45001 gatgacggtg cccgttacct ccccgatctc ctcccggcat cctgcaaggt ggatgggata
    45061 agggtgaagt tcacgggcat ggagaagagc ggggaagcca gcatctacat gtggggaaca
    45121 tcccttcgca tcctgacggc aatgcctctc ggtgaagagg taaaggccga ggggcgtatc
    45181 cagtatatcg accttgaggg cggcttctat ggcatcgtcg ccgctgatgg aaggtcttac
    45241 ctccccctga acctgaacaa agaattccag gttgacggga tgaaggtctc ttttactgcg
    45301 agaacggtcc ctgacaccct gggcatctac atgtggggaa ttccggtcac cgtcatctcc
    45361 atatctgcaa gacaggggga ggacaagtcg gcttcccagt ccctcatcgg gccatggacc
    45421 ctttccggca tggcaagggg agctgcggtc gcccctgtga tccaggggac tacgatcacg
    45481 gcagagttta ccagtgatgg cagggtcagc gggaccgcag gctgtaacct ctattttgca
    45541 ccattcgagc agagtggcac taccctccgc atcgggcctg caggttcgac agaaatgtac
    45601 tgtttcgatg cgtcgagaac gatcatgaag caggagagta cctacctgca gctcctgggg
    45661 gaggcagcat cctggaagat cagtggaggc aatctcgtga tctcggacgc atcaggccag
    45721 gagatactgg tcttttcacc cggtattgcg gatgcatccg acagaacaga acctctcgtt
    45781 gagttctgga ggaccggcgg gtttgcaggg atgaacgacc acctggaagt gtatcccgac
    45841 gggagcgttt ccctgacccg gaaggagtat tctgtcgggt tcacactctc tgaagcagac
    45901 ctcgggaccc tcaactcact tctccaggaa tcagggttca tggccctctc ctccgagtac
    45961 cgggctgctc ccgggagtgc ggatcttttc ctctaccagg tccgcgcaca tgggaagacc
    46021 gtgctcgcag aggacaccgt gataccggga tccctccagc ccctcatcga cgagcttgtc
    46081 cgcctggtgg aggagaatgc cccggatgac atcgcacccc cacgaattca ctgaacacac
    46141 ttcccccccc tttttttctc gttgtccttg ttgcccatgg ggaatacctg caccccctgg
    46201 aaggaccctc accggaagga cgacacctct tccccttttt aaggcagtcg cagttgaacg
    46261 gaccgaccga ttccttttat atcagcgcaa ccgagagtct tgtgcatgag aggggtcgga
    46321 atgaaggtat ggcgcaatat tcactggttg atgttatttg tggcaatggc ggtgctcctc
    46381 tcgttttctg gctgcatctc gcctcaggac ttcgtggcag aacccggccc cggtcctaca
    46441 gggacgctca cccctgcaac gactcccacc gccggcattc ccacagagga gggaggaatt
    46501 ggtgtactgc cagtggtccg gggagggaca tcacagtatt tcgtgaagat gccgtacgga
    46561 tacatggtga cgagtcctac taccggggtc cgtctctcag tcatagagat aaaagatgat
    46621 ctcgatgcca ctgggcagcg ggtcatcagc gggaagatca agaacgacga gaattatcgc
    46681 atcgaccata tcaccgtgta tttcaacctg tacaattcga acggaaatct catcgggaat
    46741 gcatatgcaa gcgtgaactc gcttggcccc ggaaaagtgt ggaggtttac gacaaacccg
    46801 ttcgcctaca aagattacca gtattacgag atggccggga cattcaccgc ctgaacgagg
    46861 gggattgagg ggccctccct cccacatgtc gcccgttcag accatcatgt tggtctctga
    46921 aaatggcttt atcctgacga ggatgtagtc tctcattcgg gagggtaaaa cctctgcgag
    46981 gtccccgatc gtcactcctt cgtcgaccac tgttgcccgg agccgtgacg catacgcgtc
    47041 gtatggcagc ttgacccgga gccccgtcat ctgcacggtg atgggggtcg gggagaggac
    47101 ttcgtggatg tcgtcaattt tttcggatat gatgtccatc aggcgtgcag gggagatgga
    47161 gagtgggtcc ttggccatct cctcacgttt cctgtcaagc acgcgtgtga cttctgccac
    47221 cacgtcgtca agccttgata tctcttcggc ttccttggtc cggtgcatga aacggccaac
    47281 cccgcccacg tatccatcca cgggtggatc gatgatggcc ctgaggcctc tacccgcgga
    47341 ccaccgcaga ggacaatggg gacatcgatt cccctgcgga ggactgggaa cttctgctcg
    47401 atgcaggtct cgaagtcccc gagcaggtat actgccaggt catgctcgtt gatcacatcc
    47461 ctctcctcgt cattcagctg agcgatgcgc ttcccaaagc cccttgcgag tcctaccatg
    47521 ttggtcttgg ccccgaagcg gcggatgtac tcggcaacgt cgcatgacgc atgggggagg
    47581 tgatggatct caagcgacat ggtcaccacc gcgatctcgg tccccaccag gggtgaaggg
    47641 gtgatctccc cggtaagcgg cttacccacc ttccggataa gatctatgtc ctcctttggc
    47701 acgagtgcct ggagcacgac ctcctgcgcg atgatgtgtt tctggatgat gtagccgccg
    47761 agatcctcga tgaggtcaat gatctcgtcg tggcggtaga tgcccccctt gtaggtgaca
    47821 ggaaccagga tcacaggcgc tcccccatca acgaaaattt ctccctgacc atgtcctcga
    47881 tatcctcggg gagcgtgtcc tcgcttgcga cgagatagaa ccgcccgttc ccgtaatact
    47941 gcctccgcac cttgaacccc tctggctgga tcacctgcag ggcgtaaatc aggtccttgt
    48001 agatggactc gctcgggtca aagaccggga tctcttcgag accttccgac tcatcgtcgc
    48061 caatatcaag catgatggtg aagcggtcag gctgttcgac ccgctccttt ccgaacttct
    48121 cccagagtat ccgcagcagc ggtgcaaggt atgcctcgtc gccgatggag atggtcactt
    48181 tccgctcttt cctgttcaca tcggaaaaat cgcgcacttt cacagttccc gggagcttcc
    48241 tcgttatccc ggtcgcgatg aaaaggggga cccgggggtc gatgaagatg tgcagtttct
    48301 ggatcacccg gatgaggtta tggtcgagga gcaccacgtc ggcgatgcgg cgatagtatt
    48361 ctccgccggc cttttcaggg cattccacct cgaaatactc cactgcctcc atctagtctc
    48421 ccttgataaa ccctgatgcc aggagtgcgg accccaccgc gccgatgtac tgggagtggt
    48481 gcgggaccac gatctcggtc tggaggaggt ctcccatggc ccggacaagg ccctggataa
    48541 gggaggtccc cccaaccatg atcaccgggt cctttatgtc cacctcctgg agctgttgct
    48601 cgaagacctg ctctgcaacg ctgtggcagg cggcggcggc cacgtcttcg ggactgctgc
    48661 ccgcggccag ggcgttcacc aggctctgtg tgccgaatac gatgcagtag ctgttcatgg
    48721 ggacattgcg cgagagaccc ttcattgcca gcggaccaag ctccgtgata tccacgccga
    48781 gcctctttga tgtcatctcg aggaaccggc cgctcgctcc tgcgcagata ccccccatcg
    48841 tgaacgtccc ggggatgccg tcctggacac tgatggcctt gttgtccatt cccccgatgt
    48901 cgatcacagt cgatggacct ctctgggtat cagccaggaa gaccgcaccc ttggaattga
    48961 ccgtcagttc ttcctggatg aggtccgcct tcaggtgctc cccgaccagg aaccggccgt
    49021 atccggtggt cccgatcgcc tgcagatcgt cgcgtttcac gccagcggca gagagggcct
    49081 gggataccgc atcgtcggcg ctctggagga caccagtggt ggggagccac ccggtgccga
    49141 tgatctcgtt gtcgcgcatc accactgcct tcgtggtgct ggaaccggag tcgacgccca
    49201 tggtgagccc ctgctgaact tcacgtgcaa ggagcgccct tctccgggcg atagtggtca
    49261 gggcctccat gcgggtgagg agtgtccccg cggtggtacg ctcggtgaac gagtaactga
    49321 ccaccgggag ccgggagttc tcatggatgt agcggcgaag ctcgttcctg acgattgctg
    49381 cctcggcgca cctgaaacag gttgcaatga agactgcgtc tgcctcgacc ctcccctcca
    49441 cgagcgcctt ggcgcgggct atcgcgagct tgaggtcagg gcttcgcacc tcgagaccga
    49501 actcattgaa cgaatgcctg atgtccgcaa gtttcaggtc gggaaagaag acctcggcat
    49561 ccaccgaagc cgcagccgcc tcgatctcct tctgcacccc gctgtactca gggccgcatg
    49621 agagctgcgc aatcctcacc cttgtcatgg cgaacccgcc ccaagcgtat ccaggaactt
    49681 ctttattgcg gccacgaact cgacaccctc atcctctgtc gttggatatt cgagatcgag
    49741 gacagggatg tcccgtttcc ggataaggta cttgatcagc tcgtttgtcc tggcacatcc
    49801 catgcacccg aaggagtagt ctgcattgtt gatgatgatt gccgcctccg cctcgtcgat
    49861 catggggcca tagagggaca tcctcccccg aacgccggac gggacctcca cggcggccca
    49921 tttgagaccc ttcttcggct cttctggggt gatctggagc gggggggagt cgatcccggg
    49981 tgtctggatc ctctctcgga tcttgagtgc tgcagagagg ggtttgtgcc cgaaccgtgc
    50041 caccaggtct gagaggatga ggctcgtggt gggatagaga aagaccttcg ccatgtcagg
    50101 actcctgtga ttctatgatc ttcttcaacc gggaggcatc gagcctcggt tctttcgagg
    50161 gggcaggggg atgcgggagg tcatcccgtg cccgtgaggg gagcgacgca aggcctctgg
    50221 agatgttttg gatcaatccc atctcgaact cgtggccgaa gtatcccggc ctggctcctc
    50281 cgaggttggc ccggcacctc ctggggtccc cgggggggaa ccccctgtct tttacaaaga
    50341 tgtgggtcgg gtcgagtgcc cggagcgatg cgatgattcg ttccacatcc tcctctttcc
    50401 ctgtgacctg gagtccaaaa cacgtctcct tgatcatcac cccctgcgag atctcgtatg
    50461 ccctgatcgc gaggtcggag ggcgtcatct ccggcgactc aacgaagaca tacttggtca
    50521 cggaacctgc gtaccggggc ttgtaggccg tcatggttgt ctctccctaa tgtatacgat
    50581 ctctttctcc tgcagcgcag caagcttttc gaggtcaagg acctttccga tgatgttcgt
    50641 cccttcgaaa ggctctgaag tcggtccgaa ctcgctgttc tcactggtgc gaactcccac
    50701 aagccccgac cctttcctcg agtcgttggt gatggcaagg gcccctgagg ggaccaggtc
    50761 agtgggggta ttttccggta tgatgttcac acccctcggg attttcggct tgaagaggaa
    50821 cacgtcctcg aaattgaaga agaacgggat cctccccaca tcgtggaggt gaagtcccgt
    50881 gaggtgcctg aagatgtcgc aggacgcggg ggcagcatca tcgtcgagcg tgatgtcgat
    50941 gaccttctcg agcggcacca ccgaaagttc cacggcacct gccgcaaggc tctcgatcgt
    51001 cgtggcaggc tcctgcccga cgacaacgag gtcgcggcct tccagttccc ccaggatccg
    51061 gatgcccctc tgttcagcgg tgtcaattgc ttgctcgaga ggaagcccga tgagatcaaa
    51121 gcgggagggc acgaccctgg cggagagcac gtcgccgtcc cgggcaagtc ttgccagttc
    51181 gagcccgtgc gtgacctgcc ctaccacggt atgctggggg gtcccgggca ggtcctggat
    51241 atatatgtat accgaacccc cggacctccc cgccgtcctc acgctgaccg ctccctcgcg
    51301 acgaggctgc ttcacctggt aggctacctc ggtccccgct ttcctgtcat ccctgatatg
    51361 ggtgcttgac gaccgtccta ccacgaaatg tgcatcttcg agtgcgagga ggaggtgctc
    51421 gatgctttcg gctgcggccg tggaagtcac ttcactgccg ctgtatccaa acgacgtgat
    51481 ccgcagatgc gagatcactt ccatgccgtc ctcgaggacg gtgtccgggt ccgtggtggt
    51541 gaaagaccgg ctggtgtcgg cccagttgag gacgggagat gccgtgttga cgaggtcgcc
    51601 cgtgctccac cggtcaagga cgccccgccc gctgaccacc tttccgatca cgcctccccc
    51661 tgccgcggca ccgtggtccg aagtatggcg gctccggcag aagatgaggt aggacctgtg
    51721 agggtcatac cctccgcagc cgaggaccac gtcccccctc tcgtacagcg acgcggtcct
    51781 ggccggggtg aacgatgaat gaaacgggcc gaacgcggcg gcgtacctgt cctgccagtg
    51841 cagggagagg ggaaattgca ggctggcgga tccaaatatc gcctgccccg cagggttcag
    51901 ctcgacgacc acctcgcctg cagaggtatc gagccgcatg ctcccggtcg tggcggattc
    51961 gcgggcgccc gggcggatga tcccgacgca gcacgccttg tcatggtccg gcagcaggtc
    52021 tcttaacctt gctccatccc cggtctccag cctctctccg tcgagcagga ttttgatcat
    52081 gcctgtccct tcaggaggtg acagccttgc ccatgacctc tcgctcttct tcagtaaggc
    52141 tcgcgaggac ctcggcggtc tgtccgatct gcacgatctt tcctcccctc atcaatgcaa
    52201 ggcggtcgca gatgtcccgc acaaagtcca tgtcatggga gacgacgatg aacgtctcgt
    52261 ccatctcgtc gcgggcgtgc atgatggagt gtttgacgtc gatcttggtg agggggtcca
    52321 tcgtcccggt cggctcgtcg aggattacca tcctcggctc acggatcagc acctgtgcga
    52381 gtgccacccg gtgccgctca ccttccgaga gctgcccggg gaggcggtcc aggatctccc
    52441 tgctctttgt ctcgttgaac cctgccatct tgagggtgat gatggccttc ctcattgcca
    52501 gttccttggg gaattcgaga cctatggcat ccgtgaggtt gtcaagcacc gtgcggtggg
    52561 gataaaggtc gtactcctgg tgcaggagac cgatatagcc ctttgccctc cccctgttct
    52621 cgatcccggg ctttgtcata tcgaccgaat cgtcaccgat acggatgatc atctcgccgg
    52681 ttgtcggctc gatgatcccg gagatgatcc gtgagagggt ggtctttcct gctccgctct
    52741 tcccgatgat cccgaatatc tccttctctt ttacctcgaa cgtgacaccg ttgaccgccc
    52801 gtacgactcc cctgtccact gagatatagc gcttgaagac atcccgtgcg atcatgatct
    52861 ctcctccaaa ggaggggacc tcgaactcct cggtatcgga caggtcagcc atgaaggtac
    52921 ggatcacctc ttcgggggac ccgagtgcgg cgatctttcc ctctgcaagg agcatagccc
    52981 tgtcggcaac ctcgttgatc acctgggaga agtgggaggt gacgaccatg cccatgtggt
    53041 tcgtctcggc cgcctcgatc agcatggaat ggacaaggtg ggcggtttcg gggtcgagcg
    53101 tgccggtcgg ttcgtccgca aagaggagga agggctcttt tgcaagctgg cgggcgagca
    53161 ccacccgctg tttctcccct cccgacaggt cgcgggcgat gtgcatcatg cggtgggaga
    53221 ggcgcacctg gtcgaggagg tccgcagccc tgttgatggc tttctcctgc gggtaaccga
    53281 tgtcgtccag ggcgtgaagg acgttctcta tcacgcggtc gtttccatac agggcaaatg
    53341 tcctctggaa catgatggcg gtatgccgca tgagcctccg cctcatgccc tcgtcgggct
    53401 cgttccagag gtcgatgtcc ctggccttga gcgtttttcc gcacccgggg catggctttc
    53461 cggcgagact gcctacgcca agaaaggggc agctatcgca gaccgcgacg tgatagatga
    53521 tcttcccctc tgttggcggc tgctcgacac ccctcatcag gtgcatgagc acggtctttc
    53581 cggccccact gcggccgatg atcccgagga cctccccctc accgatctca aaagaaatgt
    53641 tatgaaggac cctttttcca tcgaagtcca tacagagatt ctcaacggtg atcagcgggt
    53701 tcattctctt cctcttacta gacaatatga tgcatggtgc atattacttt tcatcttgca
    53761 tctgatcctg ccctttctca tcagtgtaca agccttttca cgatggcaac cacttcccgc
    53821 acattcttga ctacgtggtc tgcagcctgg tagagctcgg ggtgccgctc ccctgactgc
    53881 tcctctgtga gaatagcgac atccgacttc ttcatggcac aaatgtcgtt gatcccgtca
    53941 ccgaccatca gcacccggtc gtactcctcc tggaggtctg cgacaatctg ggccttgacc
    54001 gtgggcgtgg cattcccgaa gacacggtcc cgggggatgc cgaggaagtc tgccatcttc
    54061 tcgagttttg tcacccggtc gccggaagcg atgtaggtgg ggatccccat gcggtggagc
    54121 tcgctgatgg attccctcgc accctcgaac ggacggccgc cggtggtgac ggtgaactcg
    54181 atgccggaaa gacccatgtt tacgatcacc ccgctgttca tggcaaccac cgattccctt
    54241 ttgcagacat gccagacgtt ccggatgcag tcctggagat cgccgatagt gccatggggt
    54301 gactggtaga gtgcagccgc cacctcgtcg gcgacaatga ctttccgggc gcagctcacc
    54361 ccgaacccca cctggtgctc gacgagatag cgggagagag ggacttccgg cggtgtctcg
    54421 atcacctccc ttgagtggac gtggatcacg acgagtaccc gctctgggga gctgaatgtg
    54481 agcgagactg tctcgatgcc gggaaggagt tctcctttga gcacgtcctt tgcaacccgg
    54541 taggtacgga gcagggtccc ggcgctgtca aagaccacgg cgatggacat cggaaaagcg
    54601 tctcccgtat attttatgtg cgcataaccg gcactaggta catagtgccc gcaatgctag
    54661 ctgataccgc agagaagata aagcgcatgg agatccgggg ggcgggtcgg attgcaaggg
    54721 cggccgtcga agccctcgat gaatattcag gggccctcgc agaagggagc ctgcagggat
    54781 acctggagag gatggacgag gcagcggcac tcctcattgc cacccgcccc actgccgtct
    54841 ccctcccaaa tggcgtccga tcggtgatgt cggtcctgcg gcagcaattt tcgagcgttg
    54901 aggaggcaaa acaggcattc catgagcgtt gcagggcgtt tatcgtcgcg tcaggtcagg
    54961 cggtccagcg gatcggcgag atagggtccc ggcatatccg cgacggtgat gtcctgctca
    55021 cccactgcaa ctcggaagcg gcccttgcct gcatccttga ggcgaagcgg cagggaaagg
    55081 agatctcggt ctttgcaacc gaggtccgcc cccggaacca gggccacctg accatccgga
    55141 cccttgatga ggcagggatc ccgaccagtt tcatcgtcga ttctgcagcc cggtacttca
    55201 tgaacgaggt ggacctggtg gtggtgggtg ccgacgccgt ctcggtgaac ggcgcggtgg
    55261 tgaacaagat cgggacctcg cagatcgccc ttgccgcccg cgaagcgagg acgagggtga
    55321 tggtcgcggc tgagacctac aagtttgcgc cgaggaccat tctcggcgaa tacatcgaga
    55381 tcgaggagcg ggacgcggga gaggtcctct cccccgcgct ttcgagacag ctcccgcatg
    55441 tccgggtgag gaacccggcc tttgacgtga cccctgccgg ctatgtcgac cttatcgtga
    55501 ccgaaaccgg ggcaatcccg cctcagatgg cctacatcat catccgcgat gtcctgggtt
    55561 gggatatcgg cgagtattcc gggtgacctc gtccaggtgg aggccagggg gtccgggcag
    55621 ggcgaatgat tgaaagagtg cctgaccata cactatacag gccctggtga gcagccccaa
    55681 ggcggcacag ggaacagagg agatcggggg catggagtta cactttacca agctgcaggg
    55741 aaacgggaac gatttcattc tcatcgatga gtataaaggg gtggcaatcc ctgacgagat
    55801 gaaggcagag ttcgcggcgc tatactgcga ccgcaggttc ggcatcggcg gggacggggt
    55861 gctgtacctg tccctctccc agaaagctga cctgaagatg cgccttctgc agccagacgg
    55921 aagcgaggcc gagatgtgcg gaaacggtat ccgctgcttt gcgaagtacg catacgaccg
    55981 cgggtatgtc aggaagtcat gcaccgtgga gactcccgca gggatcatcc cggtcgagat
    56041 gaactaccag gacgacagct tcttcgcaac catcgggatg ccggagccga agttcatgag
    56101 gaaggatatc ccggcgaccg gcgaggggga gtataaggag gagatcgaag ggttcgaggt
    56161 gtacgcggtc aacaccggcg tgccccatgc cgt
//
LOCUS       DAOQYR010000003        50442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_755_length_50442_cov_8.677615, whole genome shotgun sequence.
ACCESSION   DAOQYR010000003 DAOQYR010000000
VERSION     DAOQYR010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 50442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50442
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_755_length_50442_cov_8.677615"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..772)
                     /locus_tag="PKM87_00570"
     CDS             complement(221..772)
                     /locus_tag="PKM87_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HNQ28589.1"
                     /translation="MHAEDDGVMTVARLMALSARTAPKGKGKDSLVCVIAGPGDLAAL
                     AGEMERLSKSLGLSIFTRDAGNVSASQACLIIGVRGQEPVGINCQGCGFPSCDEMVEA
                     TGKVNGTAAPFRGPNCVIKITDLGIAVGSAVKTASIHNVDNRVMYTAGAAALSLGWLP
                     ACTIAYGIPLSASGKSIYFDRQK"
     gene            923..1081
                     /locus_tag="PKM87_00575"
     CDS             923..1081
                     /locus_tag="PKM87_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNQ28590.1"
                     /translation="MDSYQCMICGHVYDPGRGEPSVNIPAGTDFSDLPGGWRCPVCQA
                     SPEKFRKA"
     gene            1091..2278
                     /locus_tag="PKM87_00580"
     CDS             1091..2278
                     /locus_tag="PKM87_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNQ28591.1"
                     /translation="MVVREIAPDIFSVGALDFDRRLFDSLIPLPQGTSYNSYLVKGTK
                     KTALIDTVDPTREMDLVTNLVKARVDTIDYLVVNHAEQDHSGSLPMMAELFPKAKVVV
                     NEKCLDMVTHLLDIGSERCMVINDRDTVDLGGKTLEFLITPWVHWPETMLTYAREDRV
                     LFSCDLFGSHMAESTLRVNDWKEVYHAAKRYYAEIMMPFSSSIREHMEKIDTLDIAMI
                     APSHGPIHTEPNLILDAYRDWISDKVKNEVVIPYVSMHGSTEKMVQYLTEALIDRGIR
                     VLPFNLVVTDIGELAMALVDSATVVIGTPTVIFGPHPQAVYATYLVNMLRPKTRIVSV
                     IGSFGWGGRTVEVLKGMLTRIEPELLDPVYIKGAPGAKDLKDLDNLADAIAKKHREFG
                     ILT"
     gene            2378..3340
                     /locus_tag="PKM87_00585"
     CDS             2378..3340
                     /locus_tag="PKM87_00585"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF005655.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HNQ28592.1"
                     /translation="MNHSSSFQEFLSKSGYVYIIGVAGDSGSGKTTFTAAIRDLIGED
                     LVSTITLDDYHLYGREERNALQITPLSPQANDLASLERHVARLKKGLCVEKNRYNHRT
                     GKIEGPVFFKPSRIIILEGLHPLITPGLRALLDFSFFVDPAQDVKREWKLKRDMGARG
                     YTEQEVRREMAMRESDYQSYVAPQRKYARGIIGISFSQFGRSLGWKENIYRVSLALEP
                     LSGMNRRVWMQLDLGTVLAGHSRPCSVKYLPVIEGGRLMGSIELDGGFPCESADILST
                     MLHGETGVDPRDFLPHCPLLTPTDLIQMIVCWRIISHRRSMQVR"
     gene            3385..4863
                     /locus_tag="PKM87_00590"
     CDS             3385..4863
                     /locus_tag="PKM87_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNQ28593.1"
                     /translation="MPTILVRMGRIRYFSTVLGELGEMLVFISPLTLLPLPVALIFRE
                     WEMLVPLALVPAAFFCSGFLLNYLPRKKEDVRFSAALCSVALVWLAFALISCIPFMLV
                     LDISFTDALFESMAGWTGTAFSLLRGLDSLPESLLFWRTYMQWVGGLAVISLSLTLAF
                     RSGLDTSPLFRAERRAERILPSVIANGKEIWAVYILLTVVSAGLIMVARVPLWDALNL
                     ALSGISTGGFIPRAGGILSYQNPFLEAVLIPVMIFGSTPFTLYYMTYRSRKVSFFSEE
                     QVRLLFIFLIFGALIVIADLFFLAGLPIGESFRQGIFMTTSAISTTGFQNGNPHLYPA
                     VTAVFLMMLIFIGGSSGSAAGGIRLSRIALGYRGIIWWFRRAFVRSRVLVPFKYEGRN
                     IPVEVAEPELSKSMLVIILSVITVFIATMVVLQVHITSFGLTDIVFEVVSALSSSGMS
                     TGYVNPSMPLLSKWVFIVVMWVGRLEIMPVIVLFMGLVRGRD"
     gene            4944..5762
                     /gene="cfbC"
                     /locus_tag="PKM87_00595"
     CDS             4944..5762
                     /gene="cfbC"
                     /locus_tag="PKM87_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase ATP-dependent reductase subunit"
                     /protein_id="HNQ28594.1"
                     /translation="MKQIALYGKGGIGKSTTSANLSAALSEQGLDVLQIGCDPKRDST
                     RMLMQGRLIPTVMDLVRERGDDRSLTIDDVVHSGYHGVRCVEAGGPEPGVGCAGRGII
                     ATFQILERLGALRADVVVYDVLGDVVCGGFAMPMREGYAEEVYLVTSGELMSLYAANN
                     ICKAIARLSSRARSRCRLGGVICNAKNLPREEELVSEFAEKIGSSMIQFIPRDQVVQH
                     AELNKKTVVQYAPDSGQAGTYRSLARRIMENRRLDVPSPLEIEDLEELAYGYAE"
     gene            5746..6765
                     /locus_tag="PKM87_00600"
     CDS             5746..6765
                     /locus_tag="PKM87_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HNQ28595.1"
                     /translation="MATPNDDGCTLTGALSVTTEINRCITLIHGPAGCAHHNFSLLHA
                     LHAARDLCIPPPVISTDMTEKEVIFGGEHLLEESLCRALKKKPELICVLSTCVAATIG
                     DDVDAVCRHHGEVPVVHIPTAGFLGGGFLAGHIQALLSMSRFAEPREHGLSVNLIGEK
                     TLEYEVDEHYREVVRLLSLMGVPVNVRFICGVSLGQLQALGEASLNVLRDPSMVPLGE
                     HLFRELKIPYIDSFPIGVDGTLRFLERTGRCLSLDSSEAIAAEEALQKDLIRAFAPLR
                     GQRITFNGSPMAELHLQYGYELAEALGLEVCEGGCAVPLPDPFPVGTSGIRRLLQRWR
                     RTCHA"
     gene            6758..7903
                     /locus_tag="PKM87_00605"
     CDS             6758..7903
                     /locus_tag="PKM87_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HNQ28596.1"
                     /translation="MPECAGPLWPCGLTGAAACLSGVKGMGVIVHGSAGCYFYPATTL
                     HTPIHCTSLTDHDLILGTGDALIRTIDSLQGDYSRLAVLCTCTPAIIGEDIRAVIDER
                     YGDSTRVLVLDAPGFAGEYEAGFLAALSAMRLSPGGGREDSVNIDGLSLMDPFWRGNL
                     QEIQRLIRLAGAECGATLCAGAFSCDSEISSCTIHANPDIPSCLGESLGTMLGLSGMR
                     KAVLAFEDRFGRNGGVLSELVLPDAEERIISACDKYQRRFDPPVAALFGQSASMQAVA
                     AMLSEYLCAEIACIGSRNTPSHGRFPVEMARDYATVSEMLEHERPDLVLGSSFEQAIF
                     PTAAFVGVTPPLRGAMRLHARPLAGIEGALALMESVLNACMDHKKPA"
     gene            8017..8463
                     /locus_tag="PKM87_00610"
     CDS             8017..8463
                     /locus_tag="PKM87_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28597.1"
                     /translation="MQRAGYLSRDGKRVLDEDGVPREILELNIYGARLCILIDDLIAA
                     IQEGTAAPVERVKQNWMEYLGGQAGRVQVSRSGRALNIYLMNGDRFTLSLDSLREVLG
                     YRERAARIVELPPLPPQEAARDHLITDYCRPLSHFNTHETADSMTA"
     gene            complement(8589..9704)
                     /locus_tag="PKM87_00615"
     CDS             complement(8589..9704)
                     /locus_tag="PKM87_00615"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNQ28598.1"
                     /translation="MHRIAVIGGDGIGPEIIREGTKVLEAAGDRFGFSLAFEEFDIGA
                     DRYLSTGELLTDEDIEALSRFRAIYFGAIGDDRVKPGILEKGILLALRFHFDQYINLR
                     PIRLLEGVATPLAGKNPSDIDFVVVRENTEDFYVGIGGRFRGTQKTVLGIQRELYQAR
                     FGLDLESDAEEIAYQIGVVTREGSRRVIQFACELAERRRKKLTSVDKANVLSDVYGLW
                     REVFSEVTAGYPDLATEFNFVDAVTMWFVKNPEWFDVVVTPNMFGDIITDLGAMIQGG
                     MGLAPGGNINPAGTSMFEPIHGSAPKYRGQGVANPMATIWAGSMLLDYLGEHEAAEGV
                     LKALGQSILDGVVTRDMGGSARTSDVGDYVAAMIKRG"
     gene            complement(9683..10180)
                     /locus_tag="PKM87_00620"
     CDS             complement(9683..10180)
                     /locus_tag="PKM87_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNQ28599.1"
                     /translation="MRTWKFGDNIDTDAIIPGRYLVIYDPRTLASHAFEGTRNEFAAG
                     AREGDVIVAGRNFGCGSSREHAPLALSGAGVRFIVARSFARIFYRNAINVGLLPLVCM
                     DADKVEDGSELTVNLPGGYVESGGKKYRIEPVPAFLQEIVDAGGLVEYAKDLAEVKTC
                     TGSQS"
     gene            complement(10183..11433)
                     /locus_tag="PKM87_00625"
     CDS             complement(10183..11433)
                     /locus_tag="PKM87_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNQ28600.1"
                     /translation="MAATLVEKIFSRRCGHPIRAGDVIMAPVDAAMIHDITGPLAVQK
                     FREMGGTRVFDPEKIVCLFDHQIPADSLPAAENHVYMRRFAAEQNIHNLDIKEGICHQ
                     VVMERGRAAPGDIVVGADSHTCMYGAAGAFSTGIGSTDMGFVLKFGALYFRVPTTIRL
                     EIEGAFGPRVGPKDLILAIAGEIKADGANYQALEFTGSTMEAMDMAGRMTCCNMAVEM
                     GAKAGIVPPDGVTWEYLRARRPVNPFSLTSDRDARFTSVYRFDADRLSPKVAVPHNVD
                     QVVDVEKVAGTPIDQVFIGSCTNGRFEDLREAAEVLGNRTFHDSVRVLVIPASRQEYL
                     KALRAGLIEQFVSAGALVEAPCCGPCMGGAFGLLAPGEVSLSTSNRNFRGRQGSTESM
                     VYLCSPATAAASAIRGEITDPREV"
     gene            11608..12339
                     /locus_tag="PKM87_00630"
     CDS             11608..12339
                     /locus_tag="PKM87_00630"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNQ28601.1"
                     /translation="MSRSRYPLIAVLLLLGIALLASGVSAAENGDRMIITSATAETMV
                     TPDRAQVTVSVQTENADVKVAQAENARMMDEVRRALESAGIALIDMKTTGYSIYPVYD
                     DSGSLFRQKVKYYRVTNSLQVTVRDVSRTGEVIDIAVGAGANQVDSIIFMVSEDLEQT
                     IRADLLTKAVKKARGDADVVAAASGVNITGVKEISVGGYYPPVLYENVRGGAADMKTQ
                     AAIPTPIEPGQVKITAQVTASYLIG"
     gene            12518..12970
                     /locus_tag="PKM87_00635"
     CDS             12518..12970
                     /locus_tag="PKM87_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HNQ28602.1"
                     /translation="MERTIGFKERRYIEELRILTRATALDCIIDERFERVVYVIRQGD
                     MGLAIGKKGENIRRLQNVLGKKVEMVEYSEDIKQFISNMFKPAEVTGIEESEKEGTVD
                     IVVRQKGDLGVAIGKGGATIEKARLLLRRYQGIEIGDVRQEEKPVDRQ"
     gene            12957..13250
                     /gene="hisE"
                     /locus_tag="PKM87_00640"
     CDS             12957..13250
                     /gene="hisE"
                     /locus_tag="PKM87_00640"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HNQ28603.1"
                     /translation="MTGNDILGELWEVIGNRVQNPREGSYINSLLRDPKGIDRVLEKV
                     GEEATEYILAVKNGRQREIVMEAADLQFHLLVSLKAAGIEFSSVLDELESRRR"
     gene            complement(13251..13670)
                     /locus_tag="PKM87_00645"
     CDS             complement(13251..13670)
                     /locus_tag="PKM87_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNQ28604.1"
                     /translation="MATGPLGASPAVVLKLGDDETCLPIYIGLWEAISIRNALKQEVP
                     PRPLTHDLFVEFMKSYDIMLKELVIDSLEDGVYYARLIFEKDRHEMSMDCRPSDGIAL
                     SLRCGAEILVEPGVFSGSSVCFSDISGLEDLDTYLTG"
     gene            complement(13748..14767)
                     /locus_tag="PKM87_00650"
     CDS             complement(13748..14767)
                     /locus_tag="PKM87_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HNQ28605.1"
                     /translation="MKKNEKRWLWVSLGFSLLVLVVILFFTVDANTFALLQKGDPLFF
                     ILALLIHILAMCVWALRIKWMSWGLGYQVPLLHCINLVFANLLVAAITPSQAGGEPVR
                     VHELYKAGVKVGDATAIVITERILDGFVLGLGGAFFMLVLGSMWQQIGSVYSYFLYAS
                     WIMMTAIALLFLYSVKNPLVLKRFLLRATRYIGKIRKKSRMEKYEQMIDREVDNFHNT
                     LSMFISKGKTGLFLGLLCSACFWFLEAIIVSVILIGLGQPPIVVESLIIQLIIAIIMM
                     IPLTPGGSGIAEILFTSMYSIFVPTSLVGILVVLWRSILYYSNILLGLLGSLVIVRRE
                     AADGT"
     gene            complement(14869..15441)
                     /locus_tag="PKM87_00655"
     CDS             complement(14869..15441)
                     /locus_tag="PKM87_00655"
                     /inference="COORDINATES: protein motif:HMM:NF015943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF357 domain-containing protein"
                     /protein_id="HNQ28606.1"
                     /translation="MTGRGWRDELSSRLGHLRFPVPPGTLLFSVAIELERMAGSYLRD
                     GDHFESEGDPVNAIASWSYALGWLDAGHSLGLIMAPCHDPRWIYQDIRIASTSGPKLI
                     EKVHRYHALLSTALGAVCPAAEPDTAMSRAADRFTLVAWVNREYGRSFLSSMRTPNAL
                     GAFSYGHAWLDAGVRAGLFRIERSREIFTI"
     gene            complement(15438..16187)
                     /gene="dph5"
                     /locus_tag="PKM87_00660"
     CDS             complement(15438..16187)
                     /gene="dph5"
                     /locus_tag="PKM87_00660"
                     /EC_number="2.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004164 - diphthine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine synthase"
                     /protein_id="HNQ28607.1"
                     /translation="MLTFIGLGLHDHKDVSMRGLECIREADHVFLEGYTSRLSGTSVS
                     EMQAYFRRPIRVLGREDVEQHPEIVLDPAATGNVAFLTGGDPMVSTTHADLRIRAALR
                     GIRTALIHGASISSAVCGLTGLQNYRFGKSCSLPFPAKNWSPATPIEVIGKNLSQGLH
                     TLVYLDIQEERCMTIPEAIVLLEGLAEKAKVSIPLYVGVARAGSGAPVVFAGTAEKVT
                     QADFGPPLHILVVPGPLHVVEEEYLTMFGGL"
     gene            complement(16320..17483)
                     /locus_tag="PKM87_00665"
     CDS             complement(16320..17483)
                     /locus_tag="PKM87_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HNQ28608.1"
                     /translation="MIRTGITGIDEMLGSGIPKGTRVVFSLEPGVEGRMFMFRTVATV
                     LQERKNVLIVAPHCTGRVLISECSELLNIDISGLEGEVHILDSTVRDTINSREKKTNS
                     RKIAWKKLIRETLDRHSIDVLFIYFDLLYEDLGLEKALDLMPSAGQQDRITTVVEHLN
                     LEGDSLIARFASERIFDLIISVHSAFTLVPFFNFFTLEYVSWSRMPRRSIPYTIGEGS
                     IRLYIPKIIITGPPTSGKSTFVAHASESGISVDRGDLDGYRTTVAMDLGWLHFRGFDI
                     TIFGTPGQPRFDPIIPQIVKNAMGIILLLDATRPDGLLRARELLMLAHAEKLPLVIAV
                     NKSDLPHHLDEKTIRESLNIRDDVPVFFISSLKQSDVHYVVESMVDRITRHPY"
     gene            complement(17480..18187)
                     /locus_tag="PKM87_00670"
     CDS             complement(17480..18187)
                     /locus_tag="PKM87_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28609.1"
                     /translation="MYIYKTGISVIDEVKGGISPGSNILVLAPAMSSGEKLGYSIMRP
                     TPGEYALVLSTVMRSVDLLDFFSSEKFNRDSIGVIDAVTKLSTPDISDTAQIKYVSSP
                     SDLTGIGIKFSSIVESIFNGEFPPSDQALFPPPIRFYIDSLTTLLMYRKLEVLYQFLH
                     VMTSKLKKMEAIGIFIMNNESFDERTLSLMKQLMNVVIEVKSEQQGDFLRIRGVPGVS
                     HDWMQFRIHAGTLELVT"
     gene            complement(18198..20048)
                     /gene="feoB"
                     /locus_tag="PKM87_00675"
     CDS             complement(18198..20048)
                     /gene="feoB"
                     /locus_tag="PKM87_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNQ28610.1"
                     /translation="MRLGLIGNPNVGKSLIFHQLTGLGVEVSNYPGTTVDLTRGNLCF
                     QNERLELVDLPGVYSLDGTSPEEEIVRSFILGEEVEVLVAVLDATHLERNLYLLLQVA
                     EYGKPMVAVLNMMDEAAKKGKEIDAGRLASMLGIEVIPAAAIQGKNIAEIIPAALCRA
                     RPPIVQVKYDHHIEAAIRSIQSVKPVTRLQALQALQGFGNDADALELAGALAPEIEEM
                     HRMSVPQIIAGNRHHCAHTISREVILELEEEKRDTLDSVLTSRRWGPLILVATLLSIL
                     LAVFFIGSFLEEILVEVFETMVISPFLALPLDPLIQQLGLAFLLALQAGLGIAFPFIF
                     IFYLFISLLEDSGYLTRASFLADRAMHRLGMHGQGLIPLILGFGCNVPAIMGIRLLRT
                     RRERVIASFLVSMVPCSARTVIIAGIVAAFVGIAAALSIYVITLGLVILTGLVLSRIT
                     PGEQYGMILELAPLRVPQLKNVVMKSWYRIREFLFIAMPLLIVASLILGFLQYAGFFD
                     LFQQTISPLSEAVLGLPGYATTALLFGILRKEMALETLIILAGTADLTAVLSMGQIYV
                     FAVVSVLFIPCISTIGVLARVVGARITLLVSMYTLFLGILIGALINMLIK"
     gene            complement(20045..20674)
                     /locus_tag="PKM87_00680"
     CDS             complement(20045..20674)
                     /locus_tag="PKM87_00680"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNQ28611.1"
                     /translation="MNTSIREDFLEALCVLSRRSPQLPTVSELAAYLSRGASELTSEL
                     QLMEQEGDIRILPDERVELTEKGSETGSRVMRKHQVLARFLSEVLGMDPDAASEEACI
                     LEHDISDEAFERLDRYIRRPRGRGCRMLQGRARLPSLLQFPEGSVLKVLSVRCPGGCE
                     RLADMGIFPGETVKIVHALNNKTVVVQAKGCDIALSPEIASCIFVEKRE"
     gene            20896..22476
                     /locus_tag="PKM87_00685"
     CDS             20896..22476
                     /locus_tag="PKM87_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNQ28612.1"
                     /translation="MVKKNSLADVQDAIKKRASYHLTLDCDKVDPACREYAETINTLL
                     KVAEDLRIRTQLVFAENPLPILIFDKSWNILTANQAYSDLSGIDRAALRKMNAKSFKI
                     LNQKGEGLKAAFTNKKRSFGEVTVEMPAGTRILEQYGIPILDDAGEVQSLFVIYNNIT
                     ERRTLEERLQRSISEIGEVLSNLSRGDLTRKAEIFKDDPLAKVKRDLNQSIDNLTALI
                     SEVMEMIGDLDHAMTDILSGTDEIARASQTVATTAQKTSDDAKSQIQQLEQVSREVAE
                     LNATVEKIAKNSHEMAETSRQVLNAEESARKLGKEATDKMNIVQDFSRKAMDEMVTLD
                     GKTQEISNIVKVITDIANQTNLLALNAAIEAARAGEHGRGFAVVAGEVRNLAGGSKNA
                     TKNIDNVIRDISNSSGHTVNSMKKAYEEIITGIKSVEITIEALNQMAAGVRNTAGSIA
                     EISKATDNQAVATGNVMSSVDQIHDIVVKNERNTEHLAALAEESSASIEEIASATNEI
                     KIKMGHCREIMGSFRFKA"
     gene            22488..22973
                     /locus_tag="PKM87_00690"
     CDS             22488..22973
                     /locus_tag="PKM87_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNQ28613.1"
                     /translation="MPNILEFGLDGNFYAMDIHITREIVEFLPLTPIPRTPGYIAGMT
                     NIRGEITTIIAISELIGATAGNTGPASQKFIIFMPGTARGENIGVMVDEVYSVIEVPE
                     QAIEYTENRGEGTIKSFIKGIIRVSDDSGEKEGSRVSQRLVLYLDIEKLLSYLFEMAS
                     R"
     gene            22988..23788
                     /locus_tag="PKM87_00695"
     CDS             22988..23788
                     /locus_tag="PKM87_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNQ28614.1"
                     /translation="MDEFQALLQTIQRLFHIQCTNYKEDYIKRRLASRMNSRHIPSYK
                     EYQEFLLKNMDEREALKNALTINVTKFFRDPQVFQCLKSEVLTPILRQKKRLRLWSAG
                     CSSGEEPYSYAILLYDLTTLQKDADWTVIATDIDDTILKKAKEGVYEKASLELVSESQ
                     LRRHFRQMEDGKFEVKPHIRERVKFQHHDLMSGIPISRYLDVISCRNVTIYFNDAQKN
                     DLVRMFYQGLSPGGYYVMGMSEFLSREVDGLFTPFRPLQKIFVRKDPA"
     gene            complement(23793..25040)
                     /locus_tag="PKM87_00700"
     CDS             complement(23793..25040)
                     /locus_tag="PKM87_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ28615.1"
                     /translation="MADRGDIDRIRELLLLHPKGLTIEEVAQELGINRSTASKYLNSL
                     VFSGAATIRKLGPAKLFYLREHLPLKHLINCTNDCILILDSDMVIQEINENALLLFDT
                     APEKIMGFQALHTPLAPLFDEDTCQLLKSLPESSGAESTGRLTLGCITWKLTKKIVPL
                     VFQSGNFGAGIIFRVARVAETASDTPQHPQEGIDPMYARACRQSSQLNTLFRRFSQNQ
                     LSLAIDSLKKMKQAGNPGMLRELLDEEALILENLRFHLNLLEEYLGEEIRAPGWYSLE
                     DILSRALPLQKMAHIRFFSDVRGIEVFTDRDCGRIFSTLLENSIIHGKKVTSIRVISR
                     EKDGDLSITYEDDGIGIPDKDKPSLFEWGHGIHKAHSLFLSRQMLAITDISIKETGTF
                     GKGARFELSVPAVRWRIKKDRKV"
     gene            25307..27964
                     /locus_tag="PKM87_00705"
     CDS             25307..27964
                     /locus_tag="PKM87_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HNQ28616.1"
                     /translation="MSKFSGGFSRMKIGTKILIICLVLVTVPTLILGLVAYSNSSAAI
                     SEQVDVLLETQIGDVEKMTANTYELAQQKLNGDLNVLRQTFYAYGTPSVTDGKLVLSG
                     GSRSTFVINDNNEIVDQIEQITGSKATIFQKMNGQAIRISTNVVGTDGKRALGTPVSD
                     AVYDAVIRKGETFYGTADVVGKTYITAYEPIKSASGEIIGILFVGVPEDTTYGSLKEE
                     VNSITLGETGYVYIMDSKGILISHPTLQGQNLGDQDFVKSMIANKDNVQDKPAKIAYI
                     WEGKDAVAYYMYFQPLDWIIASRVNPTDFTRPIDDIRNAIIIILVASIAIGALVSILF
                     GRSIARRMGNLVNLGNQVKEGEINAASRILEAESGNDVGGDEISEVTTAFKGVVKTIQ
                     QFSDEVATVSVAASDGRLDIRGDESKLKGDYANLIKGLNETLDAVIGPLNIAALYVER
                     ISKGDIPEKITDSYQGDFNKIKNNLNQCIENINSLVEESNMLSIAAVEGKLGKRGNAD
                     KFKNRYWEIIAGINKTLDNVVGPLNEGMRISDEFAEGNFKARFNDDIKVHGDFKKFKE
                     ALNNIGIQVSKALELVNQQISELGASAEEANASIEEVSAGSAQVAKNSAAVSGNAEQS
                     SHGINEVLKAMEDLSKTIQQVATKAEAVSALVQESNDYSRKGIDLARKTENGMNGITK
                     SSNEVNTIILEIKSQMDRIGEIVNLITDLANQTNLLALNAAIEAARAGDAGRGFAVVA
                     TEVKSLAEESRTSAEKIAEMIGNLQKQTQTAAETVAVSNAEVREGSDALKETLENFNK
                     IVESIDQISRNVTEVAAASEEQAASVEEVTASINEVNDLVGNTAKEATDAAAVSEETA
                     AAIDQITKVIGNVNNIVVKVSNEVSKFKI"
     gene            27976..28548
                     /locus_tag="PKM87_00710"
     CDS             27976..28548
                     /locus_tag="PKM87_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNQ28617.1"
                     /translation="MTMTKNASEEQGAEAAIASARRSQQGAAAGGSTGTLQVVEFLLG
                     KEYFAIDLFDVKEVVEYTTITKLPNTPAFIKGIIDLRGEITTIVDLKQRMRITEESDQ
                     PEENCRIIVLDEKNTKSKIGIMVDDVLSVSTFEMKNVDTKSASELGDEMSILGIIKKS
                     VRDKEKDKETNELIIWIDIKQLLKDIDKHM"
     gene            28906..30615
                     /gene="polX"
                     /locus_tag="PKM87_00715"
     CDS             28906..30615
                     /gene="polX"
                     /locus_tag="PKM87_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HNQ28618.1"
                     /translation="MSTEAMDVANRRISDQLHLMGQILEILDENPFKVRAYTRAAELI
                     ERLATPVADISLEELESIEGIGTAIAQKVREMVEDGTFRELEEVKGRVPPGLVEMLAL
                     EGVGPKTVRVLWKKLHVKTIEDLEKAARGHRIRALKGFGEKKEQGFLKAIANARRRQS
                     DMRMTRQEADMVIGEVRQAMSEGTYEVAGSYRRGRSTIGDIDIVTVESPSHLNRRLRS
                     IADEMIDEGERKSSFWCRGSRVDVRYTPSSTYGSMLLYLTGAKAFNIRLREIALGKGW
                     RLNEYGIRDLARGTVHEFSREEDIFAFLGMDIIPPELREDWGEIDAALSHRLPALIEG
                     SHVRGDLHVHSSWSDGSMDLEALARAGEACGYSYLLCTDHSSSLGVARGLDESALKRQ
                     SHEIEMVNRATSCQILHGVEVDILADGTTGLPSRVLKDLDVVVGSVHSAFTQETDLMT
                     RRVLSAIGNEHVDIIGHPTGRLLGQREAYAIDMQRVIESAAETGTALECNASPYRMDI
                     DDVHIREAVNHGGFISIGTDAHGPGDLAWMNYGVRLCRRGWAGPGNVINTLNMHDLLE
                     WAS"
     gene            30612..31256
                     /locus_tag="PKM87_00720"
     CDS             30612..31256
                     /locus_tag="PKM87_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HNQ28619.1"
                     /translation="MIRWLYERRLLSGLSVLPAQICFMITGDDVEEAPGKLVEVTRWC
                     VEISEAVAHAGRAPGVKGGPGGITGVTFHISTRGRDEGFSSIGRIREVAGFARLTLHH
                     GETKETMGEGMDVHVAIGKSGREEITECIRKMARDGLVNEEVSEHAIEERLTFRYTPD
                     LVIKTGGSHLTDFLIWQSVYSELFFSDVNWALFRKTDFLRALRDYQSRVRRFGK"
     gene            complement(31230..32045)
                     /gene="uppS"
                     /locus_tag="PKM87_00725"
     CDS             complement(31230..32045)
                     /gene="uppS"
                     /locus_tag="PKM87_00725"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNQ28620.1"
                     /translation="MIRELLSPLYERIVRLQCRHIPHHIAIIQDGNRRYARLQHLPRS
                     MGHRAGAARTEEMLDWSRELGIRHITLYSFSTENFQRDAGEVADLFGLFKEKFQKVTD
                     DPRVHRDGIRVQIVGDRALLPADLREVIEKAEESTRSYTGYHLNIALAYGGRNEIVRA
                     AGRILDGVKAGEYTPDEITPELVETHLHAGKDIPPVDLIIRTGNECRTSNFLPWLAGG
                     HESAVYFCAPFWPLFRKIDLLRAIRVYSQRVEAQRGMHIAAGAYTSLPETADT"
     gene            complement(32042..33034)
                     /locus_tag="PKM87_00730"
     CDS             complement(32042..33034)
                     /locus_tag="PKM87_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HNQ28621.1"
                     /translation="MPRDRSLSRGCVLCHQGAKMVLFVTGLCPRSCWYCPLSRERKGK
                     DVIYANEKRIHTPEEAAGVARLMSAFGTGITGGEPLIVLDRVQQFSRHLKTEFGESHH
                     IHLYTGIAPGREQLLDLQGLVDEIRMHPPYETWDRILESGYKEAACTAREMEFSVGIE
                     VPSLPGIGSLEVMLPFLDFLNINELEWGETNAGAMRERRLVPEDGVHNAVSGARGWAK
                     SLISRQKVHWCSSRFKDSVQLRKRLLRVARNTARPFDQITRDGTVLYGTLENGGVVPP
                     LFRQLEPDMYEVREGAIETAWWVITEFSDQLAGEKSIVERYPDRGLVVEVTPVA"
     gene            complement(33050..35200)
                     /locus_tag="PKM87_00735"
     CDS             complement(33050..35200)
                     /locus_tag="PKM87_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HNQ28622.1"
                     /translation="MGLVPSPIVLKSGTGDLKVPVYSDSKKSATALNETTSLILKSIE
                     QYRICPMNPEFRRFIEEYNSKKTQESDPEDRSLGLIPSPIDIGALTVSDTLGSNQAPV
                     SIVGSTAYGYYPSSITTATPSSWFDLRMTGKLTPVRNQEQCGACWAFSTYGSLESTNL
                     PGIRWDFSENNMKNTHGFDLAPCKGGNYLMSTAYLSRWSGAVSESSDPYVTTESSSPG
                     SVHVVQHVQDVIFLPSRTGPLDNSQIKQLLQQNGAVYSQIRWESGYFNKNLASYYYPG
                     SSSPNHAVVIVGWDDTYSRMRFSSEAPGDGAFIVRNSWGENWGEYGYFYVSYYDTCIG
                     RPSAQFLSEDPSNYDKVYQYDPLGWVTSVGVGGNSAWFANVFTSTQAEELDAVSFYSP
                     VPNTQYQLEVCSDVGSSPRGRSSILAQAGSIPYAGYHTIALSNPVALQKGQKFSVIVN
                     LITPGCSYPIAVEYPYQGYSSKATARAGESWVSSDGTSWTDITSVYPNSNVCLKAFTQ
                     ITTTPTYNPTVQPTGVPTMPSAGTDRRSPTISISSPKFMASYAPGDEVRIAWSAQDNI
                     AVSSVMVEYSPDKGLNWIPILQHAPATGTCPWAVPENLAGSITIRVTASDAAGNKGSS
                     SRSITVKSSAAASPFSWSQQKVSPGLGRNLTPPVALVSVSAGADPMTFSRYDSREQTF
                     ISPVSRSTVSGGSLSFVSPGVKTTTPTLLEQRAI"
     gene            complement(35554..36219)
                     /locus_tag="PKM87_00740"
     CDS             complement(35554..36219)
                     /locus_tag="PKM87_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28623.1"
                     /translation="MTLLLFGCLLLAIAGIGIAGAADDPGDAPAFPGQPQIPHSFYGA
                     IEAAGNPVPDRVPVEARAEGVSIGIPGNPLYSHAGGYGSADPFTPRLEVQGALSRGTG
                     LTFFVGGIQAEVQAGDSQGPWTVSYPFSPGEVTELNLRVAVEVTPDPAYKETSAQTAP
                     VTTAERTSAGGVNPSTEMMIGLIAILVILAIVAFYFGKRAEKSKREGDQESSGEGDDP
                     SQK"
     gene            complement(36358..37389)
                     /locus_tag="PKM87_00745"
     CDS             complement(36358..37389)
                     /locus_tag="PKM87_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HNQ28624.1"
                     /translation="MATKSYVDLKAEVWDRGTCAGCGACVAVCPADAICFDEAGGSLA
                     PRNIGYCKQQNDEVDCGACYDACPRVMRIDQDVLGNYLEIYSARATLEIPRRQTGGAV
                     TALILTALEQGLIDAVVTVTEDRWTKKPASVVITEAGEIVSHAGSRYNWWVPLVAALK
                     TAVMEKKCRKIAIIGVPCVVQAVNRIRTSENDLLKPFGRSIRLVIGLFCTETFDYHML
                     VEVLLKKEHHVDTWNIDRLDVRGKLEVTMRDGSLVTIPLEELQGAIRPGCRHCTDFTA
                     LFSDISAGAVGSRRGHTTLIVRNETGRAFLQEAITQNALLVGENPVDTSAIERLGRMK
                     AKNVSIDRS"
     gene            complement(37397..38137)
                     /locus_tag="PKM87_00750"
     CDS             complement(37397..38137)
                     /locus_tag="PKM87_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28625.1"
                     /translation="MSCVRIDAKGMHYTPLNKAIREAVAAGAREIEIDNVMGQRFIAD
                     GLKGDVTLVINGIPGGDLGMFMSGPTCIVRGNCDHAPGNTMDSGLIVIHGSAGDAVAH
                     SMRGGRVFVRDSIGYRGGIHMKQYRDKRPVLVIGGGFRAFLGEYMAGGLILALGNPPS
                     LPIRERGLGSGIHGGKIVIRGDVDDASLGVGARKTPVSREEMEEVVPLIREFTGYFDL
                     DPDPLLDDAYVQIQPSSSRPFASKYTWE"
     gene            complement(38145..39653)
                     /locus_tag="PKM87_00755"
     CDS             complement(38145..39653)
                     /locus_tag="PKM87_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNQ28626.1"
                     /translation="MTIGSVPVKFRISVDHDQCMVCGRCVENCPYDVFRLENGKIHVN
                     SRKCVACHRCIAMCPRDAISIRERPVDYRSHPVWTTEIREDIYNQARTGKIILAGMGN
                     AKPYPIIFDSLVLDACQVTNPSIDPLREPMELRTYLGKKPSRLEIREKGSEVEVLTPI
                     GPNLQLETPIMVGHMSYGAISLNAQASIARAVSATGTFMGTGEGGLHQSLYPYQDHMI
                     VQVASGRFGVDINYLERGAAIEIKIGQGAKPGIGGHLPGEKVCEDVSCTRMIPVGSDA
                     ISPAPHHDIYSIEDLKQLVRSLKEATEWKKPVFVKIAAVHNSAAIAAGIARSAADAVV
                     VDGFRGGTGAAPKAFRDHVGIPIEAAIASVDAKLRSQGIRNEVSLIASGGIRESADVA
                     KVIALGADAVYIGTAALVAMGCRVCGTCYRGLCPWGIATQRPDLVARLDPDIASQNVA
                     NLIHAWTLELSELMGAAGINSIESLRGNRDRLRGYLLDESIMKVLDVKPVGA"
     gene            complement(39650..40702)
                     /locus_tag="PKM87_00760"
     CDS             complement(39650..40702)
                     /locus_tag="PKM87_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase family protein"
                     /protein_id="HNQ28627.1"
                     /translation="MCGIIGVIDRSRECMDGTAIRSALSMMDERGSGEGAGYVAYGAF
                     PEFAEYYAIHVFFDTIHETKAAVDQELSKWGTIVHEEEIPTYEQQNIRKVHTPWRYFF
                     KPDARRMPRSTTPEDDIVMHLVMKVNTSIPGALIYASGKNLAIFKASGWPEEVADFYR
                     IQDYKGYIWLGHNRYPTNTPGWWGGAHPFNLLHWSVVHNGEITSYGTNRRYIESYGYR
                     CSMFTDTEVVAYLFDLLVRRHHLAPELAVKALAPPFWDEIDRMPAPDALLNRAIRLTY
                     GPALMNGPFAIVVARPDSIVGFTDRIKLRPLVTGTRGPRLYISSEEAAIRVMEPGVED
                     ITMPRAGEPIIGRIYA"
     gene            40837..42165
                     /locus_tag="PKM87_00765"
     CDS             40837..42165
                     /locus_tag="PKM87_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HNQ28628.1"
                     /translation="MSRDIVSEMLERIDADLVKFLRLQFTDIQGIPKNVAIPVQQAEK
                     ALTEGIWFDGSSIEGFARIEESDMLLKPDINTYAILPWRPADARVARFICDVYTYGNK
                     PFEGDPRYILKKQIAEAKALGYSFNTGPELEFFLFKMVDGRPSTVYQDFGGYFDLAPT
                     DWAEDVRRDIIFALTQMGFEIEASHHEVAESQHEIDFKYSTALDTADKVITFKFATKT
                     LALARNLHATFMAKPIAGINGSGMHTHGSLSKNGKNAFYDSKGEKQLSDIAYYYIGGL
                     LEHAKAVTRIANPTINSYKRLVPGYEAPVYISWSSSNRSALVRVPAARGNSTRAEFRS
                     PDPMCNPYLCFAAMLAAGLDGIRKKIEPPESTDVNIYHLSEKERRSRGIEMLPGSLIE
                     ANAELNNDDIIRNAMGTHIMEGLNSIAQLETDSFRLAVHPWELDRYLAMY"
     gene            42423..42752
                     /locus_tag="PKM87_00770"
     CDS             42423..42752
                     /locus_tag="PKM87_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNQ28629.1"
                     /translation="MKCISAIVREEKVPFIQKALEKEGIFGMTLKGVMGRGEQRGIAL
                     QYRAGIFAVDLLPKVRIDVVVPGYLEEAAVKAITTQAFTGRPGDGRIFVIDVPQSIRI
                     RTSEVEA"
     gene            43316..44512
                     /locus_tag="PKM87_00775"
     CDS             43316..44512
                     /locus_tag="PKM87_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNQ28630.1"
                     /translation="MALDTGTTAWLLASTALVMLMTPGVGFFYGGLVRRKNFISMVAL
                     SFIVFALVSIQWVVFGYSLAFGQDIGGFIGDLQFAGLQGIGMEAGDQGYPPLLFVAFQ
                     LVFAAIAMAIVTSGFAERVKLSAFLVFALLWTTLVYDPLAHWAWGGGWAMAFGALDFA
                     GGTVVHISSGFAALAVALVIGKRAGFGKYTMEPHNIPMTLLGAALLWFGWFGFNGGSA
                     LAADSIAASAFLVTNISAAAGGLAWLAASWVKGKPSSLGMVSGAIAGLVAITPAAGYV
                     TVMGSIAIGLVAGLLCYGCMLWRIRKGLDESLDAWSIHGMGGLWGAIATGVFAVAAVN
                     GYSGIIEGNVTQFLANAAGALAALVYAFVVTLVLAVAVDRVIGLRVSEEEEYVGLDIS
                     QHGERV"
     gene            44525..44854
                     /locus_tag="PKM87_00780"
     CDS             44525..44854
                     /locus_tag="PKM87_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNQ28631.1"
                     /translation="MKLIKAIIRPERLEFVKKALEEKGTYGMTVQEVSGRGDQKGIRL
                     QYRGGTMVVDLIPKVQVEVVVKDADADDVIRTIADAARTGKIGDGRIFVIPVERSIRV
                     RTGDEET"
     gene            complement(45178..45552)
                     /locus_tag="PKM87_00785"
     CDS             complement(45178..45552)
                     /locus_tag="PKM87_00785"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNQ28632.1"
                     /translation="MNAGLDHPGEGSSYEQWRTASEMEKEGRSAVAVLIHNLHSDDKW
                     DRIAAADALGNIGDRNTTGPLAKLMLDRDHDVRFATAVALGKLGGATAMQALEQASRD
                     SNHYVRLAAEEALSRLKDGLES"
     gene            45715..47250
                     /locus_tag="PKM87_00790"
     CDS             45715..47250
                     /locus_tag="PKM87_00790"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ28633.1"
                     /translation="MNSRGEWVVWPASGTDPLDAKSCLRSLLVLALILAGISAHVQGA
                     SSIDFTANVTTGNVPLNVLFTDTSSLPVIDDSRRWDFGDGTVTFDAVGTISHTYQQEG
                     TYTVRLDRTDSEGYHSLIKYDYIIATTPTPTPTPTITTVPTTSPVTTATTVIPPTTPI
                     TTVPTTAPTTQPTMQIPSEFFGEATSSGLPVPVGGRIVARIGSRNAGEIQVGSPGSYG
                     GPGPFDQRLKVYATEDEIRSGPVLITFWLEGTIQADQTAYFQSGSSQNLALSFGGTTP
                     TPTQTATPTTAPTTSPVTTSTTVTPTTPITTAPTTTPVTTIPTPTTGPTTAPTTQPTV
                     TPTWVPGMILTFESGWNQVSIPRYLLPGQDTGSIFSPVNTGGRSIWRFNGTTQQYDRI
                     YPDTHLNPTDSIWIYSTAHILVPLNFAVQQGLVERDLFSGWNGFGSLNLTEVPARDAL
                     SPVYPAWKYSYGFDPLLQRYEVSIVKGGYGIHNDSRPFVPGRGYWIFMEENVTYRASI
                     PIP"
     gene            47486..48001
                     /locus_tag="PKM87_00795"
     CDS             47486..48001
                     /locus_tag="PKM87_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin"
                     /protein_id="HNQ28634.1"
                     /translation="MTPVDRQHAVSHPVATCLLVAITLILAALLLLSIAIPSFNLSIF
                     TPTPSFLEIKSVRHVSEYGVMNYDSRVTLYHNGTLSYINDDLYAVFYRNQEKLLCVLE
                     TLNGHHFISTHHYGIQTIAGLGCRDTRWNPNEKIAIDFTDGTFHPGDLITVEVYQRPK
                     STLISRHSFTA"
     gene            48091..48630
                     /locus_tag="PKM87_00800"
     CDS             48091..48630
                     /locus_tag="PKM87_00800"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNQ28635.1"
                     /translation="MNHFTYLVIACMALSIMVAGCVQPSTTPSIPVPTSVTPVTPLPS
                     ATPLTDPALLGTWYLKAMTGPGGSSPVQTISPQMDATFSGQGDISGFAGCNHYSGQYI
                     LTGEALPNGWGISVGPVLSTLMYCAGTSDIEARFLSSLQNATSYSVTGDTLTITDHHG
                     GALVFQRTAYGPTAVPRGL"
     gene            complement(48707..49264)
                     /locus_tag="PKM87_00805"
     CDS             complement(48707..49264)
                     /locus_tag="PKM87_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF99 family protein"
                     /protein_id="HNQ28636.1"
                     /translation="MHIAKKGLRVLGIAESFSSREYSILAGVVMRKDLRVDGAALSRI
                     TVGGNDATEGVLAIYRELHRKDINVILLSGCVIAWFNILDPASIHRETGVPVVVVTYE
                     DSDGLSDDIRRHFPGDATRLARYESLGERAPLSLPTGHTAYIRPFGLPMEDANRLCRD
                     LTLDGKVPEPLRVARLVARAAMRFF"
     gene            49303..50226
                     /gene="aglJ"
                     /locus_tag="PKM87_00810"
     CDS             49303..50226
                     /gene="aglJ"
                     /locus_tag="PKM87_00810"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer glycoprotein N-glycosyltransferase AglJ"
                     /protein_id="HNQ28637.1"
                     /translation="MPIEKDQVCVLIPTLNEGPTIGALVREFRALGYTHIFVIDGNST
                     DDTRTIAAREGAGVEVQSGKGKGNALIEAFPLIEQPYILIVDGDGTYSPADAEKMLAP
                     LAEGYDQVIGDRLTDKNRDAFSRLNYFGNQVLNRLFKLAHSAYLSDILSGYRAFTRDA
                     IQQMHLHEAGFGIETEISAEAVRSGQKISVVPIEYKKRPGTPTKLNPFHDGFKITATI
                     YRLARMSNPLFYFGLIGLILMIAGVFLGIYVILEWLRQIEHLPLTIFTVLLIVIGFQI
                     FMFGVLSDMMLALHREVVRELHETRERKESR"
     gene            50356..50426
                     /locus_tag="PKM87_00815"
     tRNA            50356..50426
                     /locus_tag="PKM87_00815"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50388..50390,aa:Gly,seq:ccc)
BASE COUNT        11476 a        13639 c        14111 g        11216 t
ORIGIN      
        1 cgaagcattc agcaagtcga tggcaatcca ggacggcgtt cgccggctgt aaatttcaag
       61 tattgaaaga agaacagggg gctttccaaa agcccttaca ctttttctga gaattcctgt
      121 gaagggggtc ctaccggtcg gctggtggct gccgtgggtg caaggaaacg ataccagata
      181 ctccagttct tgattttaca ctataattat ggcgaaactg ctacttctgc cgatcaaagt
      241 aaatactctt gccggaagcc gaaagcggga tcccatacgc gatggtacat gcggggagcc
      301 atcccaggga aagggctgcg gcgcctgccg tgtacatcac cctgttgtca acattgtgga
      361 tcgacgcggt cttgaccgcg gatccgaccg caatgccaag gtctgtaatc ttgattacgc
      421 agttcggccc cctgaatggg gcagcagtcc cgttgacttt gccggttgcc tccaccatct
      481 catcgcatga gggaaacccg catccctggc agttgatgcc cacaggctcc tggcctctca
      541 ctccgataat caggcaggct tgggatgcag agacattccc cgcatccctg gtgaagatag
      601 aaagccccag gctttttgag agccgctcca tctctcctgc aagggcagcg aggtctccgg
      661 gtcccgcgat gacacacacc agcgagtctt ttcctttgcc tttcggtgca gtacgggcgg
      721 agagcgccat cagccgggca accgtcatga caccgtcgtc ttcagcatgc atgggatgtg
      781 atttggccgc cacggatatg agttgattga tatttcaaaa gaaagtggga tccttcatgg
      841 aaatcattat gctggtggat tctccaggat acaatgcgtc aattatattc tcatcatcaa
      901 cgttacagga gagaagcagc gaatggattc atatcaatgc atgatttgcg ggcatgtcta
      961 tgatcccggc agaggagaac cgtcagtgaa tatcccggcc ggaacagact tttcagacct
     1021 cccgggaggc tggaggtgcc cggtctgcca ggcaagtccc gagaagttca ggaaggcgtg
     1081 agtggtaggt atggttgtcc gggagatagc accagatata ttctccgtgg gggcgcttga
     1141 ctttgacagg cgcctcttcg acagtctgat ccccctccca caggggacca gctacaactc
     1201 gtacctcgtg aagggcacaa aaaagacggc cctcatcgac acggtcgatc ccacaaggga
     1261 gatggacctt gtcacaaacc tcgtgaaggc cagggtggac accatcgatt acctggtcgt
     1321 caaccatgcc gagcaggacc attccggttc cctgccgatg atggccgagc tctttccaaa
     1381 ggcaaaagtc gtcgtgaacg agaagtgcct cgacatggtc acgcaccttc tcgacatcgg
     1441 cagcgagcgc tgcatggtga tcaatgatcg cgacactgtc gatcttgggg gaaagacact
     1501 cgagttcctt atcacccctt gggtgcattg gcccgagacc atgctcactt atgcccggga
     1561 agaccgcgtg ctcttttcct gtgacctctt tgggtctcac atggcagaga gcacgcttcg
     1621 ggtgaatgac tggaaggagg tctaccatgc ggcaaagcgg tattatgccg agatcatgat
     1681 gcctttttct tccagcatcc gggagcacat ggagaagatc gacacccttg acatcgccat
     1741 gatcgcgccg agccacggtc ccatccacac tgaaccaaac ctcatccttg acgcctaccg
     1801 ggactggatc agcgacaagg tgaaaaacga ggtcgtcatc ccatacgtga gcatgcatgg
     1861 aagcaccgag aagatggtgc agtacctgac ggaggcactg atcgatcggg gaatacgtgt
     1921 tctcccgttc aaccttgttg tcactgatat cggggaactt gccatggcac tcgttgattc
     1981 cgcaacggtg gtaatcggaa cccctactgt catcttcggt ccgcatcctc aggcagtata
     2041 cgccacgtac cttgtcaata tgctccgccc gaagacacgc attgtttcgg tgataggatc
     2101 ttttgggtgg ggtggaagga ctgtagaggt cctgaaggga atgctcaccc gcatagagcc
     2161 ggaactcctg gacccggtgt acataaaggg agcaccgggg gcaaaggact taaaagatct
     2221 cgacaacctt gcggatgcca tcgcaaagaa gcacagggag ttcggtattc tcacgtaaag
     2281 gagaatgatc tccctttttt cattcaccag gcatgaggga ttttcccagt tgggttcacg
     2341 gggtctcttg ccaaagtgac cttaccggag agctgccatg aaccattctt ccagtttcca
     2401 ggagttcctc tcgaagtcag gctatgtgta tattatagga gtggcagggg acagcgggtc
     2461 agggaagacc accttcaccg cagccatcag ggatcttatc ggggaagacc ttgtctccac
     2521 cattaccctc gacgattatc acctctatgg aagggaggag agaaacgccc tgcagataac
     2581 ccctctctct ccgcaggcaa atgatctcgc ctcgctggaa cgccacgtcg cacggctgaa
     2641 gaaggggctt tgtgtagaga agaaccgtta caatcatcgg acagggaaaa tcgaaggccc
     2701 ggtattcttc aagccttcaa ggatcatcat ccttgaaggg ctgcatcccc tcatcacccc
     2761 cggactgcgg gcgctcctcg acttctcgtt cttcgtggac cctgcacagg acgtaaaaag
     2821 ggagtggaag ctcaagagag acatgggggc aaggggatac accgagcagg aagtccgaag
     2881 ggagatggcg atgagagagt cggattacca gtcgtatgtt gcaccgcaac gaaagtatgc
     2941 ccggggaatc attgggatct cgttctcgca gttcggccgg agccttggct ggaaggagaa
     3001 catttaccgg gtatcccttg cgcttgaacc cctttccggg atgaacagga gggtctggat
     3061 gcagcttgac ctggggacgg ttcttgccgg tcattcccgt ccgtgcagcg tcaagtatct
     3121 cccggttatt gaggggggac gtctcatggg gagcatcgaa ctcgacggcg ggttcccgtg
     3181 tgaatctgca gatatccttt ccaccatgct ccatggggag acgggagtcg atccccgcga
     3241 tttcctgccg cactgcccgc tcctgacgcc cacggacctc atacagatga tagtctgctg
     3301 gcggatcatc tcgcaccggc gctccatgca agtccggtaa aaggtggcgt acgcgggacc
     3361 aggcaaatat acatcaggct ggacgtgcca accattctgg taagaatggg caggatacgg
     3421 tatttctcca ccgttctcgg ggagctgggc gagatgctgg tcttcatcag cccgcttacc
     3481 ctcctccccc ttcccgtggc cctgatcttc cgagaatggg agatgctcgt acccctggca
     3541 cttgtcccgg cagcgttctt ctgcagcggt tttctcctca attacctgcc aaggaagaag
     3601 gaggacgtcc ggttttcggc agcgctctgt tcggttgcac tggtatggct tgcattcgcc
     3661 ctcatctcct gcatcccgtt catgctggtg ctggacatct cctttactga tgccctcttc
     3721 gagtcgatgg cagggtggac agggaccgcg ttctccctgc tcagggggct cgactccctc
     3781 cccgagagtc tcctcttctg gcggacctac atgcagtggg tcggaggcct cgcagtgatc
     3841 tccctctcgc tgactttagc atttcgatca ggcctggaca catcccctct cttcagggcc
     3901 gagaggaggg ctgaacggat cctcccgagc gtgattgcca acggaaaaga gatctgggca
     3961 gtatacatcc ttctcacggt ggtatcggcg gggcttatca tggtggcgag ggtcccgttg
     4021 tgggatgcgc tgaaccttgc cctctccggc atctctacag ggggattcat accgcgtgca
     4081 ggagggatac tctcgtatca gaaccccttc ctcgaggcgg tcctgatccc ggtgatgata
     4141 ttcggatcga cgcctttcac cctctattac atgacttacc ggagcaggaa agtctcattt
     4201 ttttcagaag agcaggtgag gctcctcttc atcttcctta tcttcggtgc cctgatcgtg
     4261 attgcggacc tcttctttct tgcaggactc cccattggcg aatcgttccg ccaggggata
     4321 ttcatgacca cttcggccat cagcaccacg gggttccaga atggaaatcc ccacctgtat
     4381 cccgcggtga ctgcggtctt tctcatgatg cttatcttta ttgggggatc gagcgggagc
     4441 gctgcagggg ggataaggct ctcaagaatt gccctcgggt accggggaat catatggtgg
     4501 ttcaggcggg cctttgtacg cagcagggtc ctggttccgt tcaagtacga gggaaggaac
     4561 atccctgtgg aggttgccga gccggaactc tcgaagagca tgcttgtaat catcctctcg
     4621 gtgatcaccg tgttcatcgc cacgatggtc gtcctgcagg tgcatatcac gtcgttcggg
     4681 ttgactgata tcgtctttga ggtcgtctct gcattgagct cgtccggcat gagcacggga
     4741 tacgtgaacc catcaatgcc gcttctctcg aaatgggtgt ttatcgtggt catgtgggtc
     4801 gggcgcctgg agatcatgcc ggtgatcgtc ctgttcatgg gtctcgttag gggaagggac
     4861 tgacctgtgg gacccgctga aatacaacct gacttttctt aacttcagaa tatctaataa
     4921 ccaaccccgt ccaataatgg gtgatgaaac agatcgccct gtacgggaaa ggcggtatcg
     4981 ggaaatcaac cacttctgcc aatctctctg ccgcgctctc cgagcagggc ctcgatgtgc
     5041 tgcagatcgg gtgcgacccc aagcgggaca gtaccaggat gctgatgcag ggcaggctca
     5101 tccccactgt tatggacctt gtgcgtgaac ggggcgacga ccgatctctc accattgacg
     5161 acgttgtaca tagtggctac catggagtca ggtgcgtgga agccgggggc ccggagccgg
     5221 gagtgggatg tgcgggcagg ggtatcatcg ccacgttcca gatactggag cggctcgggg
     5281 ctcttcgtgc cgacgtcgtg gtctacgatg tgttgggcga cgtggtctgc ggagggtttg
     5341 ccatgcctat gagggaaggg tatgcagaag aggtgtacct ggtaacttca ggcgagctta
     5401 tgtctctata cgcggcaaac aacatctgca aggccatcgc ccggctctcc tcgcgggcaa
     5461 ggagccggtg caggcttggc ggggtgatct gtaatgcaaa gaacctcccg agggaagagg
     5521 agctcgtctc cgaatttgcg gaaaagatcg gcagttccat gatccagttc attccaaggg
     5581 accaggtggt ccagcacgcg gaactgaata agaagacggt ggtccaatac gctcctgatt
     5641 cgggccaggc ggggacatac cggtcgcttg ccaggcggat catggagaac cgccgcctgg
     5701 acgtcccatc tcccctggag atagaggact tggaagaact tgcatatggc tacgccgaat
     5761 gatgacgggt gcaccctgac cggagcgctc tcggtcacca ccgagatcaa caggtgcatc
     5821 acacttattc acgggcctgc aggatgtgcc caccacaatt tttcactcct tcatgcactt
     5881 catgccgcac gggacctctg tatccctccg cctgtgatat ctaccgacat gaccgagaag
     5941 gaggttattt tcgggggcga acacctgctg gaggagtctc tctgccgtgc gctgaaaaag
     6001 aaacctgaac tgatctgtgt cctctccacc tgcgtcgctg caacgatagg ggatgacgtg
     6061 gatgcagtat gccggcacca tggtgaggtt cctgtggtgc atattcccac ggcgggattc
     6121 ctcggtggcg gatttcttgc cggacacatc caggcactcc tctcgatgtc caggttcgcg
     6181 gagccgcggg agcatgggct gtccgtcaac ctcatcggcg agaagacact tgagtacgag
     6241 gtggacgagc attaccgcga ggttgtccgc ctcctctcgc tcatgggagt cccggtgaat
     6301 gtcaggttca tatgcggtgt ttcgctcggc cagttacagg ccctcgggga ggcgtcgctg
     6361 aatgtccttc gggacccttc gatggtcccg ttaggagagc atcttttcag ggagttgaaa
     6421 atcccctata tcgactcgtt tcccatcggg gtcgatggca cgctccggtt cctcgagcgt
     6481 acgggcagat gtctttccct tgattcatcg gaggccattg cagccgaaga agcccttcag
     6541 aaggacctga taagggcttt tgctcccctg aggggacaga ggattacctt caacggatca
     6601 ccgatggctg aactgcatct gcaatatggg tacgagctcg cggaggctct cggccttgag
     6661 gtatgcgagg gaggatgtgc cgtcccgctc cctgatccat ttcctgtcgg aaccagcggt
     6721 atccggaggc tgctgcagcg atggaggaga acgtgccatg cctgagtgtg cagggccgct
     6781 atggccatgc gggctgacgg gggcagctgc ctgcctttcg ggggtaaagg ggatgggtgt
     6841 gattgtccac gggtccgcgg gatgttattt ttacccggca accacccttc ataccccgat
     6901 acactgcacg tcgctcacgg accatgacct catcctcggg acgggcgacg cgctgatccg
     6961 gaccatcgac tcgctgcagg gggattattc gcggcttgcg gtcctgtgca cctgcacacc
     7021 ggcgatcatc ggtgaggaca tccgtgcagt gatcgatgag aggtacggag attccacccg
     7081 ggtcctcgtg ctcgatgccc cgggatttgc cggtgagtac gaggcgggtt ttctggcagc
     7141 gctctctgca atgcgcctat ctccgggcgg gggccgagag gactcggtca acattgacgg
     7201 gctctcgctg atggacccct tctggagggg gaacctgcag gagatccagc gcctgatcag
     7261 gcttgccgga gcggaatgcg gtgcaacgct gtgtgcaggc gcgttctcgt gcgattcgga
     7321 gatctcttcc tgcaccatcc atgcaaaccc tgacatccca tcatgccttg gagaatctct
     7381 gggcacgatg ctcgggttgt ccggcatgag gaaggccgtc ctcgcgttcg aagacaggtt
     7441 cgggagaaac ggaggggtgc tctctgaatt ggttcttcct gatgccgaag aacggatcat
     7501 ttctgcatgc gacaagtacc agcgaaggtt tgaccccccc gtcgcagccc tcttcggcca
     7561 gtccgcgtcg atgcaggcag ttgcagccat gctgtcggag tacctgtgtg cagagatcgc
     7621 gtgcatcggt tccagaaata ccccttccca cgggcggttc cctgtagaga tggcaaggga
     7681 ctatgcgaca gtcagcgaga tgcttgaaca tgagcgccct gacctggtcc ttgggtcttc
     7741 cttcgaacag gctatattcc ccacagcggc ctttgtcggg gtgactccac cgttgagggg
     7801 tgcgatgagg cttcatgcca ggcccctggc agggatcgaa ggggcacttg cactcatgga
     7861 atcggtcctg aatgcatgca tggatcacaa aaaacctgca tgatgatgcc tggaaggatg
     7921 acttccactc ccctttatta ttcactttca ccccgcgcat atcgagtcta tttataccaa
     7981 atgctccaga caatggaacc aggagaggtg gatgacatgc aacgggcagg atatctgagc
     8041 cgggacggaa agcgagtcct tgatgaggat ggcgtccccc gcgagatcct ggagctcaac
     8101 atctacggtg caaggctctg catcctcatc gatgacctca ttgctgcaat ccaggaaggt
     8161 actgctgcac ctgtagagag ggtcaagcag aactggatgg aatatctggg aggccaggcg
     8221 gggagagtgc aggtatcacg ttctgggaga gcgctcaaca tctacctgat gaacggcgac
     8281 cggttcaccc tttctcttga ctctcttcgt gaggtgcttg gctaccggga acgtgcagcc
     8341 cggattgtag aactcccgcc gttacctccg caggaggctg cccgggacca cctcattacc
     8401 gattactgca ggccactatc ccatttcaac acgcacgaga ccgcggacag tatgacggcc
     8461 tgaacgaaga gatgtggcaa tatgggaaaa tgggggagac ccgttttctt cctgagatct
     8521 tacgccatcc ggtattgcgt gtatcttcta agagggggag ggaaaaggga gatgtcccgc
     8581 acctggtctc acccccgctt gatcatggca gccacgtaat ctcccacatc agaggtacgg
     8641 gcagacccac ccatgtcccg ggtcactacc ccatcgagga ttgactggcc gagcgctttc
     8701 agcacgcctt ctgccgcttc atgctctcca aggtaatcga ggagcatgga tccggcccag
     8761 atggtggcca tgggattggc cacaccctgt cccctgtatt ttggtgcaga tccgtgtatc
     8821 ggctcaaaca tggaggtacc cgcgggattg atgtttcctc ccggggcaag ccccatcccc
     8881 ccctgaatca tcgcaccgag atcggtgatg atatccccaa acatgttggg ggtgacgacc
     8941 acgtcgaacc attcggggtt cttcacgaac cacatagtga cagcgtctac gaagttgaac
     9001 tccgtggcga gatcggggta tccggcagtc acttcagaga atacttctct ccagagtccg
     9061 tagacatcgg agaggacgtt tgccttgtca accgaagtga gtttttttct tctcctttcg
     9121 gcgagttcgc aggcgaactg gataacccgg cgtgagcctt cacgggtgac aactccgatc
     9181 tggtaggcga tctcctctgc atcgctctcc aggtcaaggc cgaaccttgc ctggtagagt
     9241 tccctctgga tcccgagcac tgtcttctgc gtcccccgga agcgtccgcc aatcccgaca
     9301 taaaagtcct cggtgttctc ccgcaccacg acgaaatcta tgtcggatgg gttttttccc
     9361 gcaagaggtg ttgcgactcc ttccagcagg cgtatgggcc ggaggttgat gtactggtcg
     9421 aagtgaaacc tgagtgcaag gaggatcccc ttctccagta tcccgggctt caccctgtcg
     9481 tctccgatcg caccaaagta gattgcccga aacctcgaga gtgcctctat atcctcatca
     9541 gtgagcagtt ccccagtcga gagatagcga tctgccccga tatcgaactc ctcgaaggca
     9601 agggagaaac cgaaacgatc ccccgccgcc tcgaggactt tggtcccttc cctgataatc
     9661 tcggggccga ttccatcccc gcctatgact gcgatcctgt gcatgtcttc acctccgcaa
     9721 gatcttttgc atattccaca aggccgcccg cgtccactat ctcctgcagg aaggctggca
     9781 cgggctcgat ccggtacttt tttccaccgg attcaacata gccgcctggt aaattgacag
     9841 tgagttcaga tccgtcctca accttgtcag catccatgca aacgaggggg aggagaccca
     9901 cgttgatggc attccgataa aatatccgtg cgaatgacct tgccacgatg aaccggaccc
     9961 ctgcgcccga gagggcaagg ggagcatgct cgcgtgagga gccgcatccg aaattccttc
    10021 cggcaacgat gacatcccct tcacgggccc ctgccgcaaa ctcgtttcgt gttccttcga
    10081 atgcatggga cgccagcgta cgtggatcgt agatgaccag gtacctgccg ggaatgatgg
    10141 catcggtgtc gatgttgtcc ccgaacttcc atgtcctcat cttcacacct ccctcgggtc
    10201 ggtgatctct ccccggatcg cgctcgcagc tgcagtggca ggtgaacaga ggtagaccat
    10261 cgattcggtg ctgccctgcc ggccccggaa gttgcggttc gaagtcgaga gagagacctc
    10321 cccgggtgca agcaggccga atgcaccgcc catgcatggg ccgcagcagg gggcctctac
    10381 gagcgccccc gcggacacga actgctcgat caacccggcc cgcagggcct tcaggtactc
    10441 ctgccgggac gcggggatca caagtacacg gaccgagtcg tggaaggttc ggttcccgag
    10501 cacttctgcg gcctcgcgca gatcctcgaa cctgccgttt gtgcacgagc cgatgaagac
    10561 ctggtcaatc ggggtcccgg cgaccttctc aacgtcaacc acctggtcta cattgtgggg
    10621 caccgcaact ttcggtgaga gtctgtctgc atcaaacctg taaacggagg taaaccgggc
    10681 gtcgcggtcg cttgtgagag agaatgggtt cactggccgc ctggccctca ggtattccca
    10741 ggtaacgccg tcgggaggga cgatgcctgc tttcgcaccc atctctacag ccatgttgca
    10801 gcacgtcatc ctccctgcca tatccatggc ctccatcgta cttcccgtaa actcgagcgc
    10861 ctggtagttg gccccgtccg cctttatctc cccggcaatc gccaggatca ggtcttttgg
    10921 cccgacccta gggccgaaag caccttctat ctcgagcctg atggtcgtcg ggacccggaa
    10981 atacagggcc ccgaacttaa ggacgaaacc catgtcggtg gagccgatac cggtcgagaa
    11041 cgcccctgcc gccccataca tgcaggtatg ggaatcggcc ccgacgacga tatctcccgg
    11101 tgcggctctt cccctctcca tcaccacctg gtggcagatc ccttccttga tgtcaaggtt
    11161 gtggatgttc tgttcggctg caaatcgccg catgtataca tggttctcgg ctgcgggaag
    11221 agaatctgcc gggatctggt gatcaaagag acacacgatc ttttccgggt caaatactct
    11281 tgttccaccc atctcgcgaa atttctggac agccaggggt ccggtgatgt catggatcat
    11341 cgcggcgtcc acgggagcca tgatcacgtc tccagccctg atcggatgtc cgcatctcct
    11401 ggagaatatc ttctcgacaa gcgttgctgc catgtataat tcattaatgt atgcactttc
    11461 cctgttcttt tattttgggg ggagcaaatt gcaaaataaa gggcagcctt accaatgatt
    11521 tgaataagga aaaatgaaag gctgcgattt ttttcgcaat ctaggaaatg tatttgaatt
    11581 ttcaactgga tagtggaggt gatatcaatg tcgcgatcac gatatccact gattgcggtt
    11641 ctcctgctgc tggggattgc actccttgca tcaggcgtat ccgccgcaga gaacggtgac
    11701 aggatgatca tcacatctgc gaccgccgag acgatggtga ctcccgaccg ggcacaggtc
    11761 acggtctctg tccagaccga gaatgccgat gtaaaagtcg cacaggccga gaatgcccgg
    11821 atgatggatg aggtccgcag agcactggaa tcggcaggaa tagccctcat cgacatgaag
    11881 accaccggtt actctattta tcccgtctac gatgattcgg gctccctgtt ccggcagaaa
    11941 gtcaagtatt acagggttac caacagcctg caggttaccg tccgcgacgt gtcgcgaaca
    12001 ggcgaggtca ttgatatcgc agtcggcgcg ggagccaacc aggtcgacag cattatcttc
    12061 atggtgagcg aggatctcga gcaaaccata agggctgatc ttcttacgaa agcagtgaag
    12121 aaagctcgcg gcgatgccga tgtggttgcg gctgcatcag gggtaaacat aacgggcgtc
    12181 aaggagatct ccgtcggcgg ctattatccc cctgtcctct acgagaatgt ccggggaggc
    12241 gctgccgaca tgaagacgca ggcggcaatc ccgaccccca tcgaacccgg gcaggtgaag
    12301 atcactgcac aggtcacagc ctcgtatctt ataggctgaa gatcacctcc cccatttttt
    12361 ttccgcgaat ccgcggtgta cagaaatgga aatagaagga actgtgcggg ggtatccatg
    12421 atttttctct ctcacgcgaa atttgacagg gtgtttcgtt ccctttgcca atcttttaat
    12481 ccctgctccc tcccacatat acatgaagga caggtaaatg gaacgcacta taggatttaa
    12541 agaacgacga tatatcgagg aattacgtat cctgacaagg gcgaccgcgc ttgactgcat
    12601 catcgacgag aggttcgagc gggttgtcta tgtcatcagg cagggggaca tggggcttgc
    12661 aatcggaaag aagggggaga acatccgcag gctccagaac gtccttggga agaaggtgga
    12721 gatggtggaa tactcggaag atatcaagca gttcatctcc aacatgttca aacctgccga
    12781 agtgaccggg atcgaggaat cagaaaagga agggaccgtg gatatcgtgg tcaggcagaa
    12841 gggggacctc ggcgtggcaa tagggaaagg gggggccacc atagaaaagg ccaggctcct
    12901 cctccgcagg taccagggta tcgagatagg ggatgtccgg caggaggaga agccagttga
    12961 ccggcaatga tatcctcggc gagctgtggg aggtcatagg caaccgggtg caaaatccgc
    13021 gggaaggctc ctatatcaac tcgctcctgc gcgatcccaa ggggattgac cgggtgctcg
    13081 agaaggtggg cgaagaggct acagagtaca tactggcagt caagaacggc cggcaaaggg
    13141 agatcgtgat ggaagcggcc gatcttcagt tccacctgct tgtgagcctg aaagccgccg
    13201 gcatagaatt ttcttccgta ctggatgaac ttgagtcacg aaggagatag tcagccggtc
    13261 aggtaggtat ccaggtcttc cagcccggaa atatccgaga aacagaccga tgaacctgaa
    13321 aatactcccg gttctaccag tatttccgcc ccgcaacgca gggagagcgc gatcccgtca
    13381 ctgggccggc agtccatgct catctcgtgg cggtccttct cgaatatgag ccgggcatag
    13441 taaacgccgt cctcgaggga gtcgatcacg agttccttca gcatgatgtc ataagacttc
    13501 atgaactcga caaagagatc gtgggtcagc ggcctcgggg gaacctcctg cttgagtgcg
    13561 ttgcggatcg agatggcttc ccatagcccg atgtagatgg gaaggcaggt ctcatcatct
    13621 cccagtttca ggacgactgc cggggatgcg ccgagtggcc ccgtggccat atacacccct
    13681 ttcactgaac acctcacggg atccatagcc tgctacaatg gattacccgg ggtgcactta
    13741 agtgtattta cgttccatcg gcagcttccc ttcgcacgat caccaggctt ccgagaagcc
    13801 cgaggaggat attcgaatag tagagtatgc tcctccagag aactacaagg attcctacga
    13861 gggaagtagg cacaaagata ctgtacatcg acgtgaaaag tatttccgcg attcccgatc
    13921 cgcccggggt gagggggatc atcatgatga tagcgatgat cagctggatg atcagggatt
    13981 ccacgacgat cggcggctgg ccgaggccaa tgaggatgac tgagacaatg atggcttcca
    14041 ggaaccagaa gcaggcagag cacaagagcc caaggaacag gccggtcttg cccttcgaaa
    14101 tgaacatgga gagcgtgttg tgaaaattgt ctacctccct gtcgatcatc tgttcgtact
    14161 tctccattct cgactttttc cgtatcttcc cgatatacct tgttgcacgc aggagaaatc
    14221 tcttcaatac aaggggattt ttcacagaat agagaaataa tagggcgatt gcggtcatca
    14281 tgatccatga tgcatacagg aaatagctgt acacagaccc gatctgctgc cacatgcttc
    14341 ccagcaccag cataaagaac gcacctccca gcccgagcac aaatccgtcg aggatgcgct
    14401 cggtgatcac gatggcggtg gcgtccccca ctttaacacc ggctttatat agctcgtgga
    14461 cccttaccgg ctctccccct gcctgggagg gtgtgatggc tgcaaccaga aggttggcaa
    14521 agaccaggtt gatgcaatgg aggagtggca cttggtatcc aagtccccag ctcatccatt
    14581 tgatccgaag agcccagaca cacatcgcaa ggatgtggat cagcagcgcc agtatgaaga
    14641 agagcgggtc ccctttttgt aaaagggcga atgtgttcgc atcaaccgta aagaagagga
    14701 ttacgacaag gaccaggagg gagaacccga gcgacaccca aagccagcgt ttttcattct
    14761 tcttcatgat gaacgtccgc ggaaaaaggg aaatgaacgc aataggtacc gttgtaatgg
    14821 tggatatagg ttttgaattt ccaggacact cctgttgacc ccctgccttc agatggtaaa
    14881 aatctccctg gacctctcta tcctgaacag gccggcccga acccccgcgt caagccatgc
    14941 atgtccgtaa ctgaaggccc cgagtgcatt gggggttctc atggaagaga gaaatgacct
    15001 gccgtactcc cggttgaccc aggccacgag agtgaaccgg tccgcagcgc gtgacattgc
    15061 ggtatcaggt tcagcagccg ggcacactgc cccgagtgcg gttgaaagaa gtgcgtggta
    15121 acggtgcacc ttctcgatga gttttgggcc tgacgtggat gcaatgcgta tgtcctggta
    15181 aatccacctg gggtcatggc atggggccat tatcaggccg aggctgtgtc ctgcgtcaag
    15241 ccatcccagg gcgtaggacc aggatgcgat cgcgtttacg ggatctccct ccgattcgaa
    15301 gtgatcacca tcgcggaggt atgatcccgc catccgctcc aattcaatcg cgacggagaa
    15361 gaggagggtc ccgggaggga caggaaacct caggtggccg agccgcgagg agagctcgtc
    15421 cctccatccc cgcccggtca taaccccccg aacatcgtca ggtactcctc ctcgacgacg
    15481 tggagcgggc ccggcaccac caggatatgc aggggcggtc cgaaatcagc ctgcgtgacc
    15541 ttctcagccg ttccggcgaa gacgacagga gcaccagatc ctgcccttgc aactcccacg
    15601 tagaggggta tggagacctt cgccttctct gccagcccct ccagaaggac gatcgcttca
    15661 gggatggtca tgcagcgctc ttcctggata tcgaggtaga caagggtatg caggccctgc
    15721 gagaggttct tcccgataac ctcgatgggg gtcgccggag accagttttt cgcgggaaag
    15781 gggagagaac aggacttgcc gaaacggtag ttctgaagcc ccgtaagtcc gcatactgcg
    15841 cttgagatcg atgctccatg gataagcgcc gtcctgatac cacggagtgc tgcccgtatc
    15901 cgaaggtctg catgggtggt cgacaccatg gggtctcccc cggtgagaaa ggccacgttg
    15961 ccggtcgcgg cagggtcaag gacgatctcg ggatgctgct ccacatcctc gcggccaaga
    16021 acccggattg gccgcctgaa gtaggcctgc atctctgaaa ctgaggttcc tgaaagcctg
    16081 gaggtgtacc cttcaaggaa tacatggtct gcctcgcgga tacattcgag tccccgcatg
    16141 gagacatcct tatggtcgtg gagaccaagg ccgatgaatg tgagcatggt tcctgtaatt
    16201 tttaaattcc ttgcggaaat gcgccatatc ctttttgctg tgaggttaac cctgtcgttt
    16261 ccatgtattg agaatacccc tttcgtggga gatgcatcac gtggcggcta tcctccattt
    16321 cagtaagggt ggcgggtgat ccggtccacc atcgactcca cgacgtaatg cacatcggat
    16381 tgcttgagcg agctgatgaa gaatacaggg acgtcgtccc ggatgttcag gctctccctg
    16441 atcgtcttct catcaagatg gtggggaagg tccgacttgt tcaccgcgat aaccaagggg
    16501 agcttctccg cgtgtgcaag catcaggagt tctcttgccc ggaggagtcc gtcaggtctc
    16561 gttgcgtcga gcaggaggat gatgcccatc gcattcttca cgatctgcgg gataatgggg
    16621 tcgaacctcg gctggccggg ggtgccgaag atggtgatat cgaacccacg gaaatggagc
    16681 cagcccaggt ccattgcaac ggtagtgcgg tacccgtcaa ggtctccccg gtcaacagag
    16741 ataccgcttt cggatgcatg cgctacaaag gttgattttc cggatgtggg aggaccggtg
    16801 atgatgatct tggggatata gagacgtatg ctcccctctc cgatcgtata cggtattgac
    16861 ctgcggggca tccttgacca cgagacatat tccagggtga agaagttgaa gaacggcaca
    16921 agggtgaagg cggaatggac agaaattatc aggtcgaata tcctttctga cgcaaacctt
    16981 gcgatgaggc tgtcgccctc caggttcaga tgctcaacga cagtggtgat ccggtcctgt
    17041 tgtccggctg atggcatgag gtcgagtgct ttctcgagac caaggtcttc atagaggaga
    17101 tcaaaataga tgaacaaaac gtcaatggag tgcctgtcca gcgtctcccg gatgagcttc
    17161 ttccaggcaa ttttccgaga atttgtcttc ttctcacggg aattgatagt atcgcgcacc
    17221 gtgctgtcca ggatatgtac ctctccttca agcccggata tatcgatatt gagaagctcc
    17281 gaacactccg agatgagcac gcgtccagtg cagtggggtg cgacgatcag gacgttcttt
    17341 ctctcctgca ggacagtggc gacagtcctg aacatgaaca tccggccctc gacccccggt
    17401 tcaagggaga agacgaccct cgtgcccttc ggtatgcctg acccgagcat ctcgtctatt
    17461 ccggttattc ctgtccggat catgtcacca gctccagggt ccccgcgtgg attctgaact
    17521 gcatccagtc atgggatact ccgggcactc cgcggatacg aaggaagtca ccctgctgtt
    17581 cgctcttcac ctcgatcact acattcatca gctgtttcat taatgaaagt gtcctctcgt
    17641 cgaaggactc gttgttcatt ataaaaatcc caattgcctc cattttcttc agcttggagg
    17701 tcatcacatg gaggaactgg tacagcacct ccagtttccg gtacatgagg agggtggtga
    17761 gtgagtcgat gtagaacctg atcgggggcg gaaagagggc ctggtcagag gggggaaact
    17821 ctccgttgaa gatgctttca acgatggatg agaacttgat accgataccc gtgaggtcgc
    17881 ttgggctgga cacgtatttt atctgcgccg tgtcgctgat atcgggggtc gagagttttg
    17941 tcaccgcatc gatcaccccg atggaatccc ggttgaactt ttcggaagag aagaagtcga
    18001 gcaggtccac agaacgcatc actgtggaga ggacaagcgc atactccccg ggggttggcc
    18061 gcatgatcga gtatccaagt ttttccccgc tgctcatggc cggggccagg accaggatat
    18121 tgctgccggg ggaaattccc ccttttactt cgtcaataac gctgatccca gttttataga
    18181 tgtacatctc tcgatcatca ttttatgagc atgtttataa gcgctccgat gagaatgccc
    18241 aggaaaagcg tgtacatgga gacaaggagg gtgatacgtg ctccgaccac ccgtgcaagc
    18301 acaccgattg tggagatgca tggaatgaaa aggacactca ccactgcgaa tacatagatc
    18361 tgccccattg agaggactgc tgtgagatct gcagtcccgg caaggatgat cagtgtctca
    18421 agcgccatct cctttctgag tattccgaac aggagcgcag tggtggcata tcccgggagg
    18481 ccgaggaccg cttcagaaag cggagatatc gtctgctgga agagatcaaa gaaccctgca
    18541 tactgcagga accccaaaat caggctcgcg acaatgagga ggggcattgc aatgaagagg
    18601 aactcgcgta tgcggtacca cgatttcatg accacgtttt ttagctgggg aacacggagt
    18661 ggtgcgagtt cgagaatcat cccatactgt tcgcccggag tgatgcgcga gaggacgagg
    18721 ccggtgagta tgaccaggcc gagggtgatg acgtagatgc tcaaggccgc tgcaatgccc
    18781 acgaatgctg cgacaatccc tgcaattatc accgtccgtg cagagcaggg gaccatgctg
    18841 accagaaatg atgcgatgac ccgctcccgc cgggtccgca gcaggcggat tcccatgatg
    18901 gccggcacgt tgcagccgaa cccgaggatg agggggatga gcccctggcc atgcatccca
    18961 aggcggtgca tggcccggtc cgcaagaaat gaagcccggg tgaggtagcc cgagtcttcg
    19021 aggagcgaga tgaagaggta gaatatgaaa atgaatggga aggcgatgcc gagccccgcc
    19081 tgcagtgcga gaagaaacgc cagtccaagt tgctggatga gggggtccag tggaagggcg
    19141 aggaatgggg atatcaccat tgtctcgaat acctcaacaa gaatctcctc gagaaaagat
    19201 ccgatgaaga atacggccag aagaatcgaa agcagcgtgg caaccaggat gagtggcccc
    19261 cacctccggg aagtgaggac agaatcgagc gtgtcccgct tctcctcctc cagttcaagg
    19321 atcacttccc gtgaaatggt atgtgcgcag tgatgtcggt tccctgcgat gatctgtggc
    19381 accgacatgc ggtgcatctc ctctatctcg ggggccaggg ctcctgcaag ttccagtgca
    19441 tcggcatcat tcccaaaacc ctggagggcc tggagggcct ggagacgggt gactggcttg
    19501 acggactgga tgcttcgtat ggcggcctcg atatggtggt cgtatttgac ctgcacaatc
    19561 ggagggcgtg cacggcagag tgcggcaggg attatttcgg cgatattctt tccctggata
    19621 gctgctgcag ggatcacttc aattccgagc attgaggcga ggcgcccggc atcgatctct
    19681 tttcctttct tggcggcctc gtccatcatg ttcagcacgg caaccatcgg cttgccgtat
    19741 tctgcaacct ggaggaggag atagagattc cgctccaggt gggttgcatc gaggactgca
    19801 acgagtacct caacctcctc tcccaggata aatgacctga caatctcctc ttcgggagag
    19861 gtcccatcga gtgaatagac gccggggagg tcgactagtt ccaatcgttc gttctgaaaa
    19921 cagagattcc cccgtgtgag atctacggtg gtgccgggat agttgctcac ctcgaccccg
    19981 agtccagtga gctggtgaaa gatgagggat tttccaacat tggggtttcc aatgagtccc
    20041 agcctcattc ccgcttctcc acgaatatgc aggaggcgat ctcgggagag agggcgatat
    20101 cgcatccttt tgcctggact acgactgtct tgttgttcag tgcatggaca atcttgactg
    20161 tttctcccgg gaaaatgccc atgtcagcga ggcgttcaca ccccccggga catctgacag
    20221 agagaacctt cagcacgctg ccttccggaa actgaaggag cgagggaagc cttgccctgc
    20281 cctggagcat cctgcacccc cgtcctctcg gtctcctgat atacctgtca agccgctcga
    20341 acgcttcatc cgagatgtca tgttcgagga tgcacgcctc ttcagatgcc gcatcagggt
    20401 ccatcccaag gacttcagag aggaaacgcg cgagaacctg gtgtttcctc attacacggc
    20461 taccggtctc tgatcccttc tcggtcagtt caacccgctc gtccggcagg atacggatat
    20521 ccccctcctg ctccatgagc tgcagttcac tggtgagttc cgacgctccc cgcgaaagat
    20581 aggctgccag ttctgacacg gtgggaagtt gtggagatct ccgggataac acgcagagtg
    20641 cttcaagaaa atcctcccgg atcgaggtat tcatgctcaa catgttggag atggcattat
    20701 ttgagaattt gtatgaggtc agacacgaga tgtgcgaagc gtaccaaatc gcaccgatcc
    20761 cactttttta attcgtggtc ccatgcatcg tgttcaccgt tattgaatat tgaacattat
    20821 tgatactcaa atgtaataga tggtaacaat catttaatat attacttcgg agacagtatc
    20881 atgaaatgag gtggcatggt gaaaaagaac agtcttgctg acgtacagga tgccattaaa
    20941 aaaagggctt cctaccatct gacccttgat tgcgataagg tagaccctgc atgtcgcgaa
    21001 tatgcagaga caatcaacac tctattaaaa gtcgcagagg atctccgaat ccgcacgcaa
    21061 cttgtatttg ccgagaaccc gctccccatc ctgatctttg acaagagctg gaacatactc
    21121 acggcaaacc aggcatatag cgatctttct ggcattgatc gtgcagcgct cagaaagatg
    21181 aatgcgaaga gctttaaaat cctgaaccag aaaggtgagg gactcaaagc agccttcacg
    21241 aacaagaagc gcagctttgg tgaagtaacc gtggagatgc cggcaggcac acggatactt
    21301 gagcagtatg gcatccccat tctggacgat gcgggagaag tccagtctct tttcgtcatc
    21361 tacaataata tcaccgagag gcgcaccctc gaagaaaggc tgcagaggag tatatcggag
    21421 atcggcgaag ttctctcgaa cctttcccgg ggagacctca caaggaaggc cgagatattc
    21481 aaggatgatc cactggcgaa agtcaagcgt gatcttaacc agagcatcga caacctgacc
    21541 gcactcatct ccgaggtcat ggagatgatt ggagatcttg atcatgcaat gaccgatatc
    21601 ctttcaggaa cagatgagat agcccgtgcg tcccagacag tggccactac cgcccaaaag
    21661 acatctgacg atgcgaaaag ccagatccag cagcttgaac aggtgagcag ggaggttgcc
    21721 gagctgaacg ctactgtaga gaagatagca aaaaattccc acgagatggc agagacatcc
    21781 aggcaggtgc tgaatgccga ggagagtgcg cgtaaacttg ggaaagaagc cactgataag
    21841 atgaatatcg tccaggattt ctcgcggaag gccatggacg agatggtcac gctcgacgga
    21901 aagacgcagg agatcagcaa tattgtcaag gtcattaccg atattgcaaa ccagacgaac
    21961 ctcctggcat tgaatgctgc tatcgaagcg gcaagagccg gagagcacgg caggggattt
    22021 gccgtcgtgg caggtgaggt gcggaacctt gccggcgggt ccaagaatgc caccaaaaac
    22081 atcgacaatg tcatccgcga catctccaac tccagcggcc atactgtgaa ctccatgaag
    22141 aaagcctacg aagaaataat caccggtatc aaaagtgtgg agatcaccat cgaagcgttg
    22201 aaccagatgg cagcgggggt caggaatacc gcaggcagta tcgcagagat atcaaaggcc
    22261 accgataatc aggctgttgc gacaggtaat gtaatgagta gtgtggacca gattcatgat
    22321 attgtcgtga agaacgagag aaataccgaa cacctcgccg ccctcgcaga ggagagcagc
    22381 gcgtctatcg aggagatagc cagtgccacg aacgagatta agataaagat ggggcattgc
    22441 cgggagatca tgggatcatt ccggtttaaa gcctgatgga ttgcagcatg ccaaatatcc
    22501 tggagttcgg tctggacggg aatttttatg ccatggatat tcatatcacc cgggagatcg
    22561 tagagttcct cccactcact cccatcccaa ggacaccggg gtatattgcg ggaatgacaa
    22621 atatccgggg ggagataacc accattatcg cgatcagcga acttattggt gcaacggcag
    22681 gcaatacagg gcctgcatcg cagaagttca tcatattcat gcctggtacc gctcgggggg
    22741 agaatatcgg tgtaatggtg gatgaggtct atagcgttat cgaagtgcct gaacaggcaa
    22801 tcgagtatac ggaaaatcgc ggagagggca ccatcaaatc gttcattaaa ggtatcatcc
    22861 gcgtcagcga cgattcaggg gagaaagagg gaagcagggt atctcaaagg ctcgttttat
    22921 atcttgacat agagaagctc ttatcatatc tcttcgagat ggcatcgcgt tgagatcctg
    22981 aggtatcatg gacgaattcc aggctctact ccagaccata caacgtctct ttcatatcca
    23041 atgtaccaat tacaaggaag attatatcaa gcgccgcctt gcatcgagga tgaattcccg
    23101 gcatatccct tcatacaagg aataccagga attcctgctg aagaacatgg atgagcgtga
    23161 ggcattgaag aatgccctta ccatcaatgt gacgaaattt ttccgtgatc ctcaagtatt
    23221 ccagtgcttg aaatcggagg ttttgactcc aatcctgcgg cagaagaaga gattacgtct
    23281 ctggagtgca ggatgctcat caggggagga accatattca tatgccatcc ttctttatga
    23341 cctgaccact ctccagaaag atgcagactg gacggttatc gcgacagata ttgatgatac
    23401 gatacttaaa aaagcgaaag aaggagtgta tgaaaaagct tcccttgaac tggtgagcga
    23461 gagccagtta cgccgccatt tcaggcagat ggaagacgga aagttcgagg tgaagcccca
    23521 cattcgggag agagtcaagt tccagcacca tgacctgatg tccgggattc ccataagccg
    23581 ttacctggat gtcatctcgt gcaggaatgt gacgatttac ttcaacgatg cgcagaagaa
    23641 cgaccttgta cgtatgttct accagggatt gagccctggc ggatattatg tgatggggat
    23701 gtcggagttt ttaagccggg aagtggatgg cctgttcaca ccgtttcggc cattacagaa
    23761 gatatttgta cggaaagatc ctgcataaca gactagactt ttcggtcctt ttttattctc
    23821 cacctcacag cggggactga aagttcgaat cgcgccccct ttccgaaggt gcccgtctcc
    23881 tttatcgaga tatccgtgat tgccagcatc tgtcgtgaga ggaagagaga atgtgccttg
    23941 tggataccgt gaccccactc aaaaagagac ggtttatcct tgtcagggat tccaatgccg
    24001 tcatcctcgt atgtaatgga gaggtcccca tccttctctc ttgagattac ccggatggag
    24061 gtaactttct ttccgtggat aatggagttc tcaaggagcg tggagaagat gcgcccgcag
    24121 tcgcgatcag tgaaaacctc gatccccctg acatccgaaa agaaccgtat atgggccatt
    24181 ttctgcaagg gaagggcccg gctgagaata tcttcgaggg agtaccaccc cggtgccctg
    24241 atctcttctc ccaggtattc ttcgaggagg ttgaggtgaa aacgaaggtt ttcaagtatt
    24301 agtgcctcct catccaggag ttccctgagc attcctgggt ttcctgcctg cttcatcttc
    24361 ttcaggctat cgattgcaag agaaagctgg ttctgggaaa accttcgaaa caacgtattc
    24421 aactgggaag actgccggca ggctcttgca tacatagggt cgatcccttc ctgcggatgt
    24481 tgaggcgtat cgctcgcggt ctccgcaaca cgggctaccc ggaaaataat ccccgcacca
    24541 aagttccctg actggaatac caaggggacg atctttttcg tcagtttcca cgtgatgcat
    24601 ccaagcgtga ggcgccccgt actctcagca ccactgcttt caggcaaact cttcagtagt
    24661 tggcaggtgt cctcgtcgaa gagtggtgca agaggtgtgt gtagggcttg gaatcccatt
    24721 attttttctg gtgcagtatc gaagaggagg agggcattct cattgatctc ctggattacc
    24781 atatcagaat caaggataag gatgcaatcg ttcgtgcagt ttatgagatg tttcagcggc
    24841 aggtgttcac ggagatagaa aagctttgcg ggacccagtt tcctgatggt tgcagcgccg
    24901 gaaaaaacca gggaattgag gtatttcgag gcagtactcc ggttgatccc aagctcctgt
    24961 gctacttctt cgattgtaag cccttttggg tgcagcagca gcagctccct gatccggtcg
    25021 atatcccccc tgtctgccat tcatacaacc agtttgcagg tcagtaactc aatactctat
    25081 accagtaact acagacatta atgtgcaact attttaagca ttggttttgt tgatgaaaga
    25141 gacaaatatt tatatatgta aaaattgaat ttcaattatc tcttcgcgtt tcctcatcgg
    25201 gtccattctt tgagggatag cacccggaac atcgggtgca gacaagagag tggtgcatca
    25261 ctggatatat tattagcata actgtatcgt gtggaggttc cccagcatgt caaaattttc
    25321 cggcgggttt tcccggatga aaatcgggac aaagatcctt atcatatgcc tggtgcttgt
    25381 gacagtccca acgctcattc ttggactggt cgcatattcc aattccagcg cagcaatatc
    25441 agaacaggtc gacgtccttc ttgaaaccca gattggtgac gtagaaaaaa tgacggccaa
    25501 tacctatgaa ttggcgcaac agaagctgaa tggagacttg aatgtactcc ggcagacttt
    25561 ctatgcatat ggcactccca gtgtaactga tgggaagctt gtgctttcag gtgggagtcg
    25621 gagtactttt gtcatcaatg ataacaacga aattgttgat cagattgagc agatcaccgg
    25681 ttccaaggcc accattttcc agaagatgaa tggccaggcc atacgcattt ccacgaatgt
    25741 tgttggaacc gatgggaagc gggctcttgg aacccccgtt tccgatgcgg tctacgatgc
    25801 tgtgatccgt aaaggtgaga cgttctatgg tacggcagat gtagtaggta agacgtatat
    25861 cactgcctat gagccgatta agagtgcaag cggtgaaatt atagggatac tctttgtagg
    25921 ggtacctgaa gatactacat atggatccct aaaggaggaa gtaaacagca tcacactcgg
    25981 tgaaacggga tatgtctata taatggacag taagggaata ctcatttctc accccacact
    26041 tcagggccaa aaccttggtg atcaagactt cgtcaagtcc atgatcgcaa acaaggataa
    26101 tgttcaggac aagccagcaa agattgcata tatctgggag gggaaagatg ctgttgccta
    26161 ctatatgtat ttccagcccc tcgactggat aatcgcgtca cgggtcaacc ccacagactt
    26221 cacaaggcca atcgacgata tacgcaatgc gatcatcatc atcctggtgg caagtatcgc
    26281 catcggtgcc ctcgtctcaa tcctgttcgg aagatccatt gcccggagaa tgggcaatct
    26341 tgtgaacctt ggcaaccagg tcaaggaagg ggagatcaat gctgcgtcga ggatccttga
    26401 ggcagaatcc gggaatgatg tgggcgggga cgagataagc gaggttacaa ctgcctttaa
    26461 aggggtggta aagaccatac agcagttctc tgatgaagtc gcgacagtga gcgtggcagc
    26521 ttctgatggt cgtctggata tcagaggtga cgagtcgaaa cttaaaggag attatgcaaa
    26581 cctcatcaag ggcttgaacg agacgctcga tgcagttatc ggcccgttga acattgcggc
    26641 actctacgtc gagcgtataa gtaaagggga catcccggaa aagataacgg attcctacca
    26701 gggagatttc aacaagatta agaataatct gaaccagtgc attgagaata tcaattccct
    26761 tgtagaagag tcaaatatgc tatcgattgc cgcagtggaa ggaaaactgg gcaagagagg
    26821 gaatgcggat aagttcaaaa accgctactg ggagattatt gccgggatta acaagaccct
    26881 tgacaatgta gttggtcccc taaatgaagg gatgcggata tccgacgaat ttgcagaggg
    26941 aaatttcaag gcccggttca atgatgatat caaggtgcat ggggacttta agaagtttaa
    27001 agaggccctg aacaacatcg gcatccaggt ctcgaaagcc cttgaactgg tgaaccagca
    27061 gatctccgaa ctcggtgcga gtgcggaaga agcaaacgcc agcatcgagg aagtctccgc
    27121 aggatccgca caggttgcca agaactccgc tgcggtcagc ggaaatgctg aacagagcag
    27181 tcacggcatc aatgaagtgc tcaaggcgat ggaggatctc tcaaagacaa tccagcaggt
    27241 ggccaccaag gcagaggcgg tctccgcact ggtgcaggag tccaatgatt attcgaggaa
    27301 aggaattgat cttgccagga agaccgagaa cggcatgaat ggaatcacca agtcctcgaa
    27361 tgaagtgaac accatcatcc ttgagatcaa gtcccagatg gacaggatag gggagatcgt
    27421 aaacctgatc actgaccttg ccaaccagac caacctcctg gctctgaatg ccgccataga
    27481 ggcagccagg gcgggagatg cagggcgtgg ctttgcggtg gtggccacag aagtcaagtc
    27541 gcttgccgag gagtcaagga cctctgcaga gaagattgcg gagatgattg gaaatctgca
    27601 gaagcagacc cagactgctg ccgagacagt tgcggtctcc aatgcagagg tcagggaagg
    27661 aagcgatgcg ctgaaggaga cgcttgagaa tttcaataag atcgtcgaat cgatcgacca
    27721 gatcagcagg aacgtcaccg aggtggcagc cgcgtccgag gagcaggcag catcggtaga
    27781 agaggtcact gcaagcatca acgaagtgaa cgacttggtc ggcaataccg cgaaagaagc
    27841 tacagacgct gctgcagtca gcgaagagac cgcagctgca atcgatcaga tcaccaaggt
    27901 catcggcaac gtcaacaata tcgtggtaaa ggtctcgaac gaagtctcaa agttcaaaat
    27961 ttaattggtg aggccatgac catgacaaag aacgcaagcg aagaacaggg ggctgaagcg
    28021 gccatcgctt ccgcacgcag aagccagcag ggagctgccg ccggaggcag tacaggaact
    28081 cttcaggtgg tggaatttct cctgggcaag gagtattttg ccatcgatct cttcgatgtc
    28141 aaagaggtgg tagagtatac caccattacg aaacttccga acacccctgc gttcatcaag
    28201 gggatcatcg acctcagggg tgagatcacc acgattgtcg atctcaagca gcggatgcgt
    28261 atcacagaag agtcggacca gcccgaggag aactgcagga ttattgtcct tgacgagaaa
    28321 aacaccaagt caaagattgg gataatggtg gacgatgtcc tctcggtctc gacgtttgag
    28381 atgaagaacg tggataccaa gtccgcatcg gagcttggtg acgagatgtc gatccttggc
    28441 atcattaaaa agagtgtcag ggacaaggag aaggacaagg agacgaacga actcattatc
    28501 tggatcgaca tcaagcagct tctcaaagat atcgacaagc acatgtgatg cgggcccacc
    28561 actcttttta gtccattctt cactttttgc gattgatcca ggtcctcttc gttctttctg
    28621 caagattgaa aaacggcgaa aagtccttta tccgtgcact gcgaggagtc cagaattcat
    28681 tccatgtcgg aaaccggggc attgcatccc ccaccacata attcctcaca gaaaatagtt
    28741 ttaaaaaccc aagttacgaa aagccagcag ggaaaccaac caaacgtgag ggaacttctc
    28801 atcgagcaat acaacgcatg tgtctggatt gtcgctattc gcacccatta aaagcgaatg
    28861 catatcaaga gatcaatacc aaaatgaggg tacggcagtt ggggggtgag cacggaagca
    28921 atggatgttg ccaacaggag gatatcggat caactgcatc tcatggggca gatcctcgaa
    28981 atacttgacg agaacccgtt caaggtccgt gcatacaccc gcgccgctga acttatcgag
    29041 aggcttgcaa ctcccgttgc agacatctcg ctggaggaac tcgagtctat cgaggggatt
    29101 gggaccgcta tcgcacagaa agtcagggag atggtcgagg acgggacgtt ccgcgaactc
    29161 gaagaagtca aaggcagagt ccccccgggt cttgttgaga tgcttgccct cgagggggta
    29221 ggaccaaaga ccgtgagggt actctggaag aagcttcatg tcaagaccat cgaagacctc
    29281 gagaaggcag cccggggtca tcgtatccgg gccctgaagg ggtttggcga gaagaaggag
    29341 cagggctttc tcaaagcgat tgcgaatgcc cggcgccgcc agtccgacat gagaatgacc
    29401 cggcaggaag cggacatggt gatcggcgag gtcagacagg cgatgtcgga gggaacgtac
    29461 gaggttgcgg gaagttatcg caggggcagg agcacgatag gcgacatcga tattgtcaca
    29521 gtcgagagcc cctcccatct taacaggcga cttcggagca tcgcagacga gatgattgac
    29581 gaaggcgaaa ggaagagctc gttctggtgc cgtggttcga gagtagatgt caggtatact
    29641 ccttcgtcca cgtacggctc catgctcctc tacctcacgg gtgcgaaggc cttcaacatc
    29701 cgcctcaggg agatcgcgct tgggaaggga tggcggctga acgagtatgg aatccgcgac
    29761 cttgcccgag gtactgtcca tgaattctcc cgcgaggagg atatcttcgc attcctcggg
    29821 atggacataa ttcccccgga actccgcgaa gactggggcg agatcgatgc tgccctttcg
    29881 caccgtcttc ctgctctgat cgaggggtcc catgtgagag gggacctcca tgtgcacagt
    29941 tcctggagtg acgggagcat ggatctcgag gctctcgccc gtgcaggaga ggcgtgtggg
    30001 tatagctatc tcctctgtac cgatcactcg tcatccctcg gggttgcccg cggactcgac
    30061 gaatccgcac tcaagcggca gtcgcacgag attgaaatgg tgaaccgcgc cacctcctgc
    30121 cagattctcc atggagtcga ggtggacatc cttgcagacg ggacaaccgg gcttccctcc
    30181 cgggttctca aagatctcga tgtggtcgtc ggatcggtgc actcggcatt tacccaggag
    30241 accgatctga tgacacggcg cgtcctctcc gctatcggga atgaacatgt tgatatcatc
    30301 ggccacccta ccgggagact gcttggccag cgggaggcat acgcgatcga tatgcagcgg
    30361 gtaatcgaat ctgcagcaga gacaggtacg gcactcgaat gcaatgcttc accttaccgg
    30421 atggacatcg atgatgttca catcagggag gccgtgaacc acgggggttt tatctcgatc
    30481 ggcaccgatg cccatggccc cggcgacctt gcctggatga attacggggt gcggctctgc
    30541 cggaggggat gggcaggacc cgggaatgta atcaataccc tgaatatgca tgatctgctg
    30601 gagtgggcat catgatccgg tggctgtatg aacgaaggct tctttcaggg ctctcggtcc
    30661 tccctgccca gatctgcttc atgataacgg gagatgacgt ggaagaggcg ccaggcaagc
    30721 tggtcgaagt cacgcggtgg tgcgtggaga tttcggaggc ggttgcccac gcaggcaggg
    30781 cacctggcgt gaaaggtggg ccaggcggga tcacgggggt gacattccat ataagcaccc
    30841 ggggcaggga tgagggattc tccagcatcg gcagaatcag ggaggttgcc ggctttgcac
    30901 gactcaccct gcaccacggg gagaccaaag agacgatggg ggaagggatg gacgtgcacg
    30961 tcgcaatagg gaagagcgga agggaggaga tcaccgagtg catcaggaag atggcgaggg
    31021 atggacttgt gaatgaggag gtgtcggaac acgccatcga ggagcggctc accttccggt
    31081 atactcctga ccttgtgata aagacaggtg ggagccacct gaccgacttc ctcatctggc
    31141 agtcggtgta ttccgagctc ttcttctcgg acgtgaactg ggcgcttttc aggaagacag
    31201 actttttacg ggcgctccgg gactaccagt cacgtgtccg ccgtttcggg aagtgatgtg
    31261 tatgcccctg cagcgatgtg catgccccgc tgtgcctcaa ctctctggga gtatacccgt
    31321 atcgccctga gaagatcgat cttccggaac aaaggccaga atggggcaca gaaatagacc
    31381 gcggactcgt ggccgcctgc aagccatgga aggaaattcg aggtcctgca ctcgtttcct
    31441 gtgcgaatga tcaggtcgac gggggggatg tccttccctg catggagatg agtctcgacg
    31501 agctcggggg taatctcgtc aggagtgtat tctcctgcct ttaccccatc caggatccgc
    31561 ccggcggccc gcacgatctc gtttcggcca ccataggcca gcgcgatgtt gagatggtag
    31621 cctgtgtagg accgtgtgga ttcttccgcc ttctcgatca cctcgcgcag gtctgccggg
    31681 aggagtgccc ggtcacccac gatctgcacc ctgatgccgt cgcggtgaac cctcgggtcg
    31741 tcggtgacct tctggaactt ctccttgaag aggccaaaga gatccgcgac ctcccctgca
    31801 tctctctgga agttttctgt cgagaatgag taaagggtga tatgccggat tccaagttca
    31861 cgtgaccagt ccagcatctc ctcagtccgt gcggcgcctg cccgatgccc cattgagcgc
    31921 gggaggtgct ggagcctggc atacctgcgg ttcccgtcct gtatgatggc gatgtggtgg
    31981 ggaatgtgcc ggcactggag gcgcacgatg cgctcataga gtggggagag cagctcgcgg
    32041 atcatgcaac cggtgtcacc tccacgacaa ggcccctgtc ggggtatcgc tccacgatgc
    32101 tcttctcccc tgccagctgg tcggaaaact cggtgattac ccaccaggcg gtctcgatgg
    32161 caccctccct cacttcatac atgtcaggtt caagctgacg gaagagggga ggaacgaccc
    32221 ccccgttttc aagggtacca tagagaacgg taccgtcgcg tgtaatctgg tcaaatggcc
    32281 tcgcagtgtt gcgggcgacc cggagcagcc tctttctgag ctggacagag tctttgaacc
    32341 gcgatgaaca ccagtgcact ttctgccggc tgataagact ctttgcccat cctcgtgcgc
    32401 cgctgaccgc gttgtgcact ccatcctcgg ggaccaggcg acgttcgcgc atggccccgg
    32461 cgttggtctc accccattcg agttcgttga tattgagaaa gtccaggaac ggaagcatca
    32521 cttcgaggct cccgatgccc gggagcgagg ggacctcgat ccccactgag aactccatct
    32581 ccctggcagt gcatgccgcc tctttatacc cgctctcaag gatcctgtcc catgtctcat
    32641 atggggggtg catccggatc tcgtcgacca gtccctgcag atccaaaagc tgctctctcc
    32701 ctggtgcaat tcctgtatag aggtgaatgt ggtgcgattc cccaaactcc gtcttcaggt
    32761 gccggctgaa ctgttgcacc ctgtcaagga caatcagagg ttcccctccg gtaatgcctg
    32821 tgccgaacgc gctcatgagg cgggcgaccc ctgcggcttc ttccggagta tggatgcgct
    32881 tctcgttggc atagatcaca tccttcccct ttcgttcccg ggagaggggg cagtaccagc
    32941 aggagcgggg gcagaggccc gtgacaaaga ggaccatctt ggcaccctgg tggcagagca
    33001 cgcatccccg ggagagcgac ctgtctcttg gcatctctct tccttccgat tatatcgctc
    33061 tctgctctaa taaggttgga gttgtggtct tcaccccggg tgatacgaac gaaagactcc
    33121 ctccggaaac agtggaccgg gagaccggtg aaatgaaggt ctgttccctg ctgtcgtaac
    33181 gggaaaacgt catcgggtca gcgccggcag atacgctcac gagtgcaacc ggtggtgtca
    33241 gattccgacc caaccccgga gacaccttct gctgcgacca ggagaatgga cttgcggctg
    33301 cactggattt gacggttatg gacctgcttg aggaaccctt atttcctgcg gcatcgcttg
    33361 cggttacgcg gattgtgatg ctccccgcaa ggttctccgg gacagcccag ggacaggtgc
    33421 cggttgcagg agcgtgctgg agaatgggaa tccagttcag gcccttgtca ggagaatatt
    33481 ctaccatcac actggacacg gcgatattgt cctgcgctga ccatgcaatc ctgacttcgt
    33541 ctcccggtgc gtaactggcc ataaatttcg gggaggagat gctgatggtc gggcttctcc
    33601 ggtcagtccc tgcggaaggc atcgtgggaa ctccagtcgg ctggacagtg gggttgtacg
    33661 tgggggttgt cgttatctgc gtgaatgcct tcaggcacac gtttgaattc gggtagactg
    33721 acgtgatatc tgtccagctc gtcccatcgc tgctcaccca gctctcccct gcccgtgcgg
    33781 tcgccttcga tgagtatccc tggtaagggt attcaaccgc gattgggtaa ctgcagcccg
    33841 gcgtgataag attgacgatg acggagaatt tctgcccctt ctgaagggca accgggttgg
    33901 agagggcaat tgtgtgatag cctgcatatg ggatactccc tgcctgggca aggattgagg
    33961 atctcccccg gggtgaggat cccacgtcgc tgcatacctc gagctggtac tgggtattcg
    34021 ggacaggtga atagaagctt accgcatcca actcttcagc ctgtgtcgac gtaaatacgt
    34081 tggcaaacca tgcgctgttt ccccccaccc cgacgctcgt tacccatcca agcgggtcat
    34141 actggtagac cttatcataa ttggatgggt cttcagagag aaactgggcg gaaggccgtc
    34201 cgatgcaggt atcataatag gagacgtaga aatatccata ctcaccccag ttttcacccc
    34261 agctgttcct gacaatgaat gccccatcgc cgggtgcttc actggagaac ctcatcctgc
    34321 tgtaggtgtc gtcccatccg acaattacta ccgcgtggtt tggagaggat gagccgggat
    34381 agtaatagga tgcaaggttt ttgttgaagt atccgctctc ccaccgtatc tgtgaataca
    34441 ctgctccatt ctgctggagc agctgcttta tctgggagtt atcgagaggc cctgtccttg
    34501 agggcaggaa tatgacgtcc tggacatgct gcacaacgtg gacactcccg ggcgaactgc
    34561 tttctgtcgt gacgtaggga tcggacgact cgcttaccgc ccctgaccag cgtgagaggt
    34621 acgcggtgga catgaggtaa tttcctccct tgcagggtgc aaggtcgaac ccatgcgtgt
    34681 ttttcatgtt gttctctgaa aagtcccacc gtatcccggg caggttcgtg gattcgagag
    34741 aaccatatgt ggagaatgcc cagcaggccc cgcactgttc ctggttccgg acaggagtga
    34801 gttttcctgt catgcgcagg tcgaaccagc ttgatggggt agcagtggtg attgatgatg
    34861 gataataacc gtatgccgtt gatccaacga tggaaacggg cgcttgatta gatcccaagg
    34921 tatcagatac agtgagtgcg ccgatatcta tgggagaagg gatgagtcca agggagcgat
    34981 cttccgggtc cgactcctgc gttttcttcg agttatattc ctcgatgaat ctcctgaact
    35041 cggggttcat agggcatatc cggtattgtt caatggattt caatatcagg gaggtggtct
    35101 cgtttaaggc cgttgcagac ttctttgagt ccgaatagac ggggactttc agatctcctg
    35161 tccccgattt caggacaatg ggcgatggga cgaggcccat gctatgacct tccctgtcaa
    35221 acgttgtgcc tccaacgctc gcgttgtatt tgaaatatgt aaaaaaatcg ggactcacat
    35281 ccggcttttc caggtatgtc ctgtcttctg cagagacctg gaatagcagc actgagcaga
    35341 tcgcaagaaa aattattact tttttaacca tttgcattca tcccgtattg aatcgttgtt
    35401 ctatcctgac tggtgcagaa ttcctccgcg gtccctggat atgatgtatc attcagatac
    35461 ttaattctgc attccagtcg cctctgggta tgtcgtatcc tgatatcatg ataaaatgat
    35521 aggatgggga tggttcaggc ggtctgagca gtatcatttc tggctggggt catccccctc
    35581 tccgctgctc tcctggtccc cttctctctt tgatttttcc gctcttttcc caaaatagaa
    35641 tgcaacgatc gcgagtatta ccagtatcgc aatgaggccg atcatcatct ccgtcgaggg
    35701 gttcactcct ccggcagagg ttctttctgc agttgtgaca ggggcagtct gcgccgaagt
    35761 ctccttgtat gcgggatcag gggtcacttc gactgcaacc ctgaggttga gttcagtcac
    35821 ttcacctgga gagaatggat agctgacggt ccagggccct tgtgaatccc cggcctgcac
    35881 ctctgcctgt atcccgccga cgaagaaggt caggccggtc ccccgggaga gagcaccctg
    35941 gacttccagc cggggtgtga agggatcggc ggagccgtat cctccggcat gtgagtagag
    36001 ggggtttcct ggaatcccta tggaaacgcc ctcggcccgt gcttcaaccg ggacacggtc
    36061 tggaaccggg ttccctgccg cctcgatggc tccatagaac gaatgaggga tttgcggctg
    36121 gcccgggaag gccggggcat cccccggatc atcagctgca cctgcgattc cgatccctgc
    36181 aattgcaagg aggaggcatc cgaaaagaag tagtgtcatt ctcatgagtt catatccctg
    36241 ccttttcaca taatctcctc tgaaatccca tttaactttt cctgaccgtt cacgcgcatg
    36301 aatatgcacg gaataaacct ctgacaataa atgatcgtgg attatggatg atcacactca
    36361 ggatcggtca atggacacat tctttgcttt catccggccg agtctctcga ttgcgcttgt
    36421 atccaccggg ttctcaccga ccagcagggc attctgtgta attgcctcct ggaggaaggc
    36481 tctcccggtc tcgttccgga cgatgagcgt ggtatggcct ctcctgctcc cgaccgcacc
    36541 agccgagatg tccgagaaga gcgcagtaaa atctgtgcag tgacggcatc ccgggcggat
    36601 tgccccctgc agttcctcca ggggaattgt gaccagggac ccgtcgcgca tggtcacctc
    36661 aagttttccg cgcacgtcga ggcgatcgat gttccaggta tcgacgtggt gctctttttt
    36721 gagcaggact tccacaagca tgtggtagtc gaaggtctcc gtacagaaaa gcccgatgac
    36781 aaggcgtata cttctcccga atggtttcag gagatcgttt tcgctcgtcc gtatccggtt
    36841 gacggcctgc acgacgcatg gcacccctat tattgcgatc ttccggcact tcttttccat
    36901 gaccgcggtc ttgagtgctg cgacgagcgg aacccaccag ttgtaacgac tcccggcatg
    36961 actgacgatc tctcctgcct ccgtgataac caccgatgca ggctttttcg tccagcggtc
    37021 ctcggtaaca gtgacgaccg catcgatcaa cccctgttca agggcagtca gaatgagtgc
    37081 ggtcaccgct ccccctgtct gtctccgggg tatctccagg gttgcccttg cagagtatat
    37141 ctcaaggtaa ttcccaagaa catcctgatc aatcctcatc acgcgggggc aggcgtcata
    37201 gcacgcgccg cagtccactt cgtcgttctg ctgcttgcag tagccgatgt ttctcggtgc
    37261 gagcgaccct cctgcctcgt cgaagcagat ggcatccgcg gggcataccg cgacacatgc
    37321 accgcacccc gcacaggtgc ccctgtccca gacctcggct ttcaggtcca cataactctt
    37381 ggttgccatg aacttctcac tcccaggtat acttgcttgc aaacggcctg ctgcttgacg
    37441 gttggatctg tacgtatgca tcatccagaa gcgggtcggg gtccaggtca aaatagcctg
    37501 tgaattccct gataagcgga acgacctcct ccatctcttc cctggagacc ggagttttcc
    37561 tggcccctac tccaagggag gcgtcgtcaa cgtcgccccg gatgacgatc ttgcctccat
    37621 ggatcccgga cccaagcccg cgttccctta tcgggaggga aggtggatta cccagggcga
    37681 gaatcagccc acctgccata tattccccaa ggaacgcacg aaaccccccc cctatcacga
    37741 ggaccgggcg cttgtcgcgg tactgcttca tgtggatgcc gccgcggtat ccgatggagt
    37801 ccctgacaaa gactcttccg ccccgcatgg agtgggcaac cgcgtcgccg gcactcccgt
    37861 ggatgacgat gagccccgag tccatcgtgt ttcccggggc atggtcacag ttcccgcgga
    37921 cgatgcaggt gggcccgctc atgaacatcc cgaggtcacc cccggggatt ccattgatca
    37981 ccagcgtgac gtcccccttg agtccgtcag cgatgaatct ctggcccata acgttgtcga
    38041 tctctatctc acgggcaccc gcggcgaccg cttcccggat cgccttgttg aggggcgtat
    38101 aatgcattcc cttggcatca attctcacgc agctcatggc agcatcaggc ccccacgggt
    38161 ttgacatcga gcactttcat gatggactca tcaaggaggt atccccttaa cctgtcccgg
    38221 ttgcccctga gtgactcgat gctgttgata cccgcggcac ccatcagctc cgagagttcc
    38281 agggtccagg catggatgag gttggccacg ttttgggagg cgatgtcggg atccagccgc
    38341 gccacgaggt ccggtctctg cgtggcgatt ccccacgggc acaggccacg gtagcaggtt
    38401 ccacacacgc ggcatcccat cgcgacgagt gcagcagtcc cgatgtagac tgcgtcggcg
    38461 ccgagtgcga tcaccttcgc tacatctgca ctctcgcgga tgcccccgct tgcgatgagg
    38521 gaaacctcgt tcctgatacc ttgggagcgg agtttcgcgt caacgcttgc gatcgccgcc
    38581 tcgatcggta tccccacatg gtcccggaag gcttttggag ccgcgccggt gcctcccctg
    38641 aacccgtcaa caaccaccgc atccgcagca gaccttgcga tccctgcagc gatggctgca
    38701 gagttatgca cggcggcaat cttcacgaag accggtttct tccattccgt ggcttctttc
    38761 aggctccgca ccagctgctt gaggtcctcg atactgtaga tatcgtgatg gggagcgggg
    38821 ctgatcgcat cgcttccgac cggtatcatc cttgtgcatg agacatcttc gcacaccttc
    38881 tcccccggca ggtgcccgcc gatacccggt tttgcgccct ggcctatctt gatctcgatg
    38941 gctgcacccc tctcaaggta gttgatgtcc accccgaaac ggcccgaagc cacctggaca
    39001 atcatgtgat cctggtatgg gtagagggac tggtgcagtc ccccttcacc cgtccccatg
    39061 aaggtccccg ttgcagagac ggccctcgct atgctcgcct gggcgttcag gctgattgcg
    39121 ccgtagctca tatgacctac catgatcggg gtctccagct gaaggttcgg gccgatgggg
    39181 gtgagcacct ccacttcgct gcctttctcc cggatctcaa gccttgaagg ctttttcccc
    39241 agataggtgc gaagttccat cggttcacgc agcgggtcga tactcgggtt cgtgacctgg
    39301 cacgcgtcga ggaccaggct gtcgaagatg atgggatatg gctttgcgtt ccccatcccg
    39361 gcgaggatga tctttccggt ccgcgcctgg ttatagatgt cttcccggat ttccgtggtc
    39421 cataccgggt gactgcggta atccaccggg cgttcgcgga tgctgatggc atcgcgtggg
    39481 cacatcgcga tgcaccggtg acatgccacg catttcctgg agttcacgtg gatttttcca
    39541 ttctccaggc ggaagacgtc atagggacag ttctcgacgc agcggccgca caccatgcac
    39601 tggtcatggt cgaccgatat cctgaacttt accggcacgc ttccgattgt catgcatata
    39661 tcctcccgat aatgggctct ccggcgcgtg gcatggtgat gtcctcgaca cccggttcca
    39721 tcaccctgat ggctgcttcc tcgctcgaga tgtacagccg gggcccacgt gtgcctgtga
    39781 caaggggccg gagcttgatg cgatcggtga acccgacgat tgagtcaggc cgtgcgacaa
    39841 cgatggcaaa cggcccgttc atgagggcgg ggccataagt gagccttatc gccctgttca
    39901 ggagtgcatc aggtgcgggc atgcgatcga tctcgtccca gaacgggggg gcaagcgcct
    39961 tcaccgcaag ttcgggggca aggtggtggc ggcgtacgag gaggtcgaag agataggcga
    40021 ccacctcggt atcggtgaac atcgagcagc ggtagccata gctctcgatg taccggcggt
    40081 tggtcccgta tgaggtgatc tccccgttgt ggaccacgct ccaatggagg aggttgaagg
    40141 ggtgtgcgcc gccccaccat cccggggtgt tggtcgggta gcggttgtgg ccaagccaga
    40201 tatatccctt gtaatcctgt atgcggtaga agtcagcaac ctcttcaggc cacccggatg
    40261 ccttgaagat cgcaaggttc ttcccggatg catagatgag ggccccggga atgctggtat
    40321 tcaccttcat cacgaggtgc atgacgatat cgtcttccgg ggtggtactc ctcggcattc
    40381 gccttgcatc gggcttgaag aaataccgcc agggagtgtg gaccttcctg atattctgct
    40441 gctcgtaggt tgggatctcc tcttcgtgca cgatggttcc ccacttcgag agctcctggt
    40501 cgactgctgc ctttgtctcg tggattgtgt cgaagaagac atgtatcgca taatattcgg
    40561 cgaattccgg gaatgcaccg tatgccacgt accctgcgcc ttcaccactt cctcgttcat
    40621 ccatcatgga aagtgccgac cgtatcgccg tcccgtccat gcattccctt gacctgtcga
    40681 tgactccaat aattccacac atatcaatca cgggtcgtct ggcctttaca tgaatcctgc
    40741 accggacagg gagttatagc ccgactgcgt tatgtgatct cttatagggg ctgattggtt
    40801 aaatatatat ctgattaggt agaagttaat ttccatatga gcagagacat cgtgtctgag
    40861 atgcttgaac ggatcgacgc agacctggtc aagttcctcc gcctgcagtt cacggatata
    40921 cagggaatac caaaaaacgt ggcaatcccg gttcaacagg cagaaaaagc ccttaccgag
    40981 gggatctggt ttgatggatc ttccattgag ggatttgcac gaatcgaaga atcagacatg
    41041 ctcctgaaac cggatatcaa tacctatgcc atccttccct ggagacctgc ggatgcgagg
    41101 gtcgcccgtt tcatctgcga tgtctatacc tacggcaaca agcccttcga aggcgaccca
    41161 aggtacatcc tgaagaagca gatcgcagag gcaaaggcgt tgggctactc attcaatacc
    41221 ggccccgagc ttgaattctt cctcttcaag atggtcgacg gcaggccttc cacagtatac
    41281 caggatttcg gcggatattt tgaccttgcc cccacggact gggctgaaga tgtgcgcagg
    41341 gatatcatct ttgcactgac ccagatgggc tttgagatag aagcatctca tcacgaggtt
    41401 gcagagagcc agcatgagat cgatttcaaa tacagcaccg cactggatac cgccgacaag
    41461 gtgattacct tcaagttcgc gacaaagacc cttgcacttg ccaggaacct gcatgcaacc
    41521 ttcatggcca agcctattgc agggatcaac gggagtggca tgcataccca tggctctctt
    41581 tcgaagaatg ggaagaatgc attctatgat tccaagggcg agaagcagct ctcagatatt
    41641 gcctactact acatcggcgg gcttctcgag cacgcaaagg cagtcacaag gattgcgaat
    41701 cccacaatca actcgtacaa gcgactcgtc ccgggatacg aggctccggt gtacatcagc
    41761 tggagttcat cgaacaggtc ggcgctggtc agggtacccg cggcgcgtgg aaacagcacc
    41821 agggcggaat tccgtagccc ggaccccatg tgcaacccat acctttgctt tgcagccatg
    41881 ctggcagcag gccttgacgg tatcaggaag aagatcgaac cccccgaaag taccgacgtg
    41941 aacatatatc atctctctga aaaggagagg agatcacgcg ggatcgagat gctcccgggc
    42001 agtctgatcg aggcgaacgc ggaactcaac aacgacgata taatcagaaa cgcgatgggc
    42061 acacatatca tggaaggcct caattcaatt gcccaactgg agactgattc gttcaggctc
    42121 gcggtgcatc cctgggagct tgaccgctac ctggcaatgt attaattttt tttctcctaa
    42181 agaaaatgga aagttactta agtgttaacc aataatttcc ttccatcata ttatgggaac
    42241 agacaacgca tggatgctga ccgcaaaaga gatgaaagga atccagtcct gttggtgagt
    42301 cctctcgaaa aggagatagc cgggacctgc tggcttccct tcttttctgg aaacgaagga
    42361 aaagcatcac ttcatgacac ggagcctgga tgcattccca cacaaaccgg ggatgatgcg
    42421 agttgaaatg catttcggca attgtccgcg aagagaaggt cccattcatt cagaaagccc
    42481 tggagaaaga ggggattttt ggaatgacac taaaaggggt gatggggagg ggagagcagc
    42541 ggggaattgc gctccagtac cgcgcgggga tcttcgcagt cgatcttttg ccgaaggtga
    42601 ggatcgacgt tgtcgttcct ggatatctcg aagaggcggc agtgaaggct attactactc
    42661 aggccttcac tggcagacct ggtgatggga ggatatttgt gattgatgta ccgcaatcaa
    42721 tccgcatacg gacaagtgaa gtggaggcct gatatgtagc catacgattg gacctcccga
    42781 aaaaaatctc ctggccggga ttataccggt caatgtgctc tagaatgccg gggaatttcc
    42841 ccggcgttcc gcgtctataa aaaggagatt taccttttgt tagaagatca attttctatc
    42901 tccctctcac atcagattct tggagtttca gcatcttacc atttattgga ctaaaaagaa
    42961 aactataaat accaaggaac cacacttcta cccatcaaat gagtttactc ctacccagtc
    43021 accagggcag aagtccgcgt cccttcactc tcatagagaa ggggtgggga atacctgtaa
    43081 aaacgggtgt acaaccggat gaccggaatc acgggagcca tttgatatac aggatcacgg
    43141 aaggatgctg ccggacaagg agctaaataa tccctccgca caggagatgg gctctcccat
    43201 gaattgaggg gtggaaaggt gcctggtcct gaagtacccg gccagggtcc ggaacagtat
    43261 cgttgcaggc tgactatctg gaacagaatc agaagaagga ttcaggagat tgacaatggc
    43321 tttagataca gggaccaccg catggctgct tgcttctacg gccctggtga tgttgatgac
    43381 accgggcgtg ggcttcttct atgggggtct ggtcaggaga aagaacttca tctcgatggt
    43441 ggccctctcg ttcatcgtct ttgcgctggt cagcatccag tgggtggtgt tcggttattc
    43501 actggcattt gggcaggata ttggtgggtt catcggggat ctccagtttg caggcctgca
    43561 gggtatcggg atggaggccg gggaccaggg gtatccgcca ctgctctttg ttgccttcca
    43621 gctggtcttc gcggctatcg ccatggctat cgtgacatcg ggctttgccg agagggtgaa
    43681 actctctgcg ttcctggtct ttgccctcct gtggacaacc cttgtctacg accccctagc
    43741 gcactgggca tggggtggag gctgggccat ggcattcggc gcacttgatt tcgccggcgg
    43801 cacggtcgtc cacatcagct cgggctttgc ggccctggca gtggcactgg taattggaaa
    43861 aagagcaggt ttcgggaagt acaccatgga gccgcacaac atccccatga ccctcctcgg
    43921 tgctgcgctc ctgtggtttg ggtggttcgg attcaatggg gggagtgcgc ttgcggctga
    43981 cagcatcgca gcgagtgcat tcctggtcac aaacatctcc gctgcggctg gaggccttgc
    44041 ctggctcgct gcaagctggg taaagggaaa accaagttcg cttggcatgg tgagcggggc
    44101 aattgcaggg ctcgtcgcaa tcaccccagc tgcaggatac gtgacggtga tggggagtat
    44161 cgcaatcggg cttgtcgccg ggctgctctg ctatggctgc atgctgtggc gaatcaggaa
    44221 aggcctggac gagagcctcg acgcctggtc catacacgga atgggcgggc tgtggggtgc
    44281 gatcgcgact ggtgtgtttg cggtcgctgc cgtgaacgga tactcgggga tcattgaagg
    44341 gaatgtcacc cagttccttg ccaacgcagc cggtgccctt gccgcgctcg tgtacgcctt
    44401 cgtggtcaca cttgtcctgg ctgtggcagt ggacagggtg ataggtcttc gggtcagcga
    44461 agaagaggag tatgtcggcc ttgatatttc gcagcacggc gagcgggttt aggaagtggt
    44521 ggcgatgaag ctgataaagg ccattatccg gcctgaaagg ctggagttcg tgaaaaaggc
    44581 cctcgaagag aagggtacgt acgggatgac tgtgcaggaa gtgtccggga ggggggatca
    44641 gaaagggatc cggctccagt atcgcggggg gaccatggtg gtggatctca tcccgaaggt
    44701 ccaggtggaa gtggtggtaa aggatgccga tgccgatgat gttatcagga caattgccga
    44761 tgcagcacgt accggcaaga ttggagacgg acggatcttc gtgattcccg tggagcggtc
    44821 catccgggtt cgcactggcg atgaggagac ctgaatcgcc ccctgtccac ctcttctttt
    44881 ttaaaaccga gtaggccatg aattcctctg tcttgtcagc actaatagaa gagcatgata
    44941 cgaggccctg catcagtttg tatcaatggt gcatgccact tgcggggcac atgcgcatgc
    45001 ggataaaagg ttgcagatgt ggtatctaaa aaaaaactat atgattctct tcgagacaca
    45061 cgcaaacaca atcatgaaaa tggcgcagat attttcatag taaccatcaa tgaccgccgg
    45121 gcggtcgagc ataacgcatg aaaatgccgc aggcatattc attcgtaaaa atggagttca
    45181 ggattccagg ccgtccttca ggcggctgag cgcttcttct gcagccagcc tgacatagtg
    45241 gtttgaatcc ctgctcgcct gttccagtgc ctgcatggcg gtggcgccgc ccagttttcc
    45301 cagggcgact gcggttgcaa acctgacatc gtggtctctg tccagcatga gctttgcgag
    45361 gggacctgtt gtgttcctat ccccaatatt tcccagtgca tctgccgcag cgatacgatc
    45421 ccacttgtca tcgctatgca ggttgtgtat caacactgcc acggcagaac gcccttcttt
    45481 ttccatctcc gaggcggtcc tccattgctc gtatgaagaa ccctctcccg ggtggtcgag
    45541 cccggcattc atgtgacatc ccccttgacc ctggcgggtc aaccgcagat aggtgtgagg
    45601 atacttcaag ctatgcatcg cacctttgca cccctgggga cgttctcgtt cccggcgccg
    45661 gtgtccttgt gcaatgagaa tatatatctg cctgtgtgga gacacattgc gtgaatgaat
    45721 tcccgcggtg agtgggtggt gtggccggcg tccgggactg acccgctgga cgcgaagtca
    45781 tgcctgcgga gcctcctcgt ccttgccctt atccttgcag gcatatcggc ccatgtgcag
    45841 ggcgcatcca gtatcgattt tactgcaaat gtcactaccg gaaatgttcc cctcaacgtc
    45901 ctcttcaccg acacgagcag tcttccggta atcgatgact caagaagatg ggactttgga
    45961 gacgggaccg tcacgttcga tgcggtcggc accatatccc acacctacca acaggaagga
    46021 acctacacgg tccgcctgga ccgaacagac agcgaaggat accattccct cataaaatat
    46081 gactacatca tcgctaccac gcctacgcca acgcccaccc ctaccatcac caccgtcccc
    46141 accacatcac ccgtgacaac tgcgacgaca gttattcctc ccaccactcc catcaccaca
    46201 gtgcccacta cagccccgac tacacagccg acgatgcaga tcccctcgga atttttcggc
    46261 gaggcgacct cgtccggact ccctgtgccg gttgggggac gtattgtggc aaggattgga
    46321 tcaaggaacg ccggggagat ccaggtgggc agtcccgggt catacggggg tcccgggccc
    46381 tttgaccagc gattgaaagt gtatgcaaca gaggatgaga tcaggagcgg tcctgtcctt
    46441 atcaccttct ggctggaggg gacgatccag gcagaccaga ccgcatattt ccagtcagga
    46501 tcttctcaga accttgccct ctcctttggc ggaaccaccc caactcccac acagaccgcg
    46561 acccccacca cggctcccac cacttcgcct gtcactacct cgaccacggt tactccgacc
    46621 acccccatca ctaccgcccc gacaaccacc ccggtcacga ctattcccac ccccaccacg
    46681 gggccgacaa ccgcaccgac tacccaaccc acggtcaccc cgacatgggt gccgggaatg
    46741 atcctcacgt tcgaatctgg atggaaccag gtctccatcc ccaggtatct ccttccgggc
    46801 caggatacgg gaagcatatt ctcccctgta aataccgggg ggagatcgat ctggcgcttc
    46861 aatggcacaa cacagcaata cgacaggatt tatccagaca cgcatctcaa cccgactgac
    46921 agcatctgga tatacagcac ggcgcatata ctcgtcccgc ttaatttcgc cgttcagcag
    46981 ggcctcgtgg aacgtgacct cttttcaggg tggaacggat ttggcagcct caacctgacc
    47041 gaggttcctg caagggatgc actctcacct gtttacccgg catggaagta cagctatggc
    47101 ttcgatcccc tgctccagcg ctatgaggtg tcgatcgtga agggcgggta tgggattcac
    47161 aacgactcga ggccatttgt cccgggcagg ggatactgga tcttcatgga ggagaacgta
    47221 acataccggg catccattcc tattccctga ttcttttttt ctgaatgcaa caagaatgaa
    47281 agcgttctct gcccctgtgc ggaaatcaga tgccattggc tcgaaatgcc caggagtccc
    47341 ggcggttcgc gcgggattcc gcagatctta tgaatggtaa actgaccaaa aaacccaggc
    47401 tgtcagggaa cctatctgtc agggagtaaa gccagccatt tcaattcgca atatttattc
    47461 gtataagaga aaaacctgcg atagtttgac tccagtagat cgtcagcatg ccgtgtcaca
    47521 ccccgtggcc acctgcctgt tggttgcaat taccctcatc cttgcggcgc ttctccttct
    47581 ctccatcgct atcccgtcat tcaacttgtc tatatttact ccgaccccct ccttccttga
    47641 gattaaaagt gtccggcacg tgagcgagta tggcgttatg aactacgatt cgcgtgtcac
    47701 actctaccac aatggcacgc tctcttatat caatgacgac ctctatgcgg tcttttatcg
    47761 aaaccaggag aagcttctct gcgtcttaga gacgctcaac gggcaccact tcatctctac
    47821 ccatcattat ggcatacaga ctattgcggg gcttggatgc cgggatacgc gttggaatcc
    47881 gaacgaaaag atcgctatag atttcacgga tgggacgttt cacccaggag acctgataac
    47941 cgtcgaagta taccagcggc cgaaatcaac ccttatctca cgccacagct ttactgcctg
    48001 atgtcacatg ttgcggagcc gagaaaaaaa tatgaaacat ggcgctccac cagcgatatc
    48061 tctttcaccg taaaacactc ctatgcgtat atgaaccatt tcacgtacct ggtgattgcc
    48121 tgcatggccc tttcaattat ggtcgcaggg tgtgtacagc cgtcaaccac gccatcaatc
    48181 cccgtgccaa ccagcgtgac tccggtgacc cccctcccct ctgcgactcc cctgacggat
    48241 cctgcccttc tcgggacatg gtatctcaag gcaatgactg gccctggcgg ttcttcacct
    48301 gtccagacca tcagccccca gatggacgcg acattcagcg gacaggggga tatatcgggc
    48361 tttgcggggt gcaaccatta cagcgggcaa tatattctca cgggagaagc actccccaat
    48421 ggatggggca tttcggtagg gccggtattg tccacattga tgtactgtgc cgggacaagc
    48481 gatattgaag cccggttcct ctcctcactg cagaacgcaa cgagttactc tgtcaccggg
    48541 gacacactta ccatcaccga ccaccatgga ggcgccctcg tattccagag gactgcctac
    48601 ggccccactg cggtgccgcg aggcctctga cttttttttt tgcgcccgga ttttcgaata
    48661 actcctgcaa gcttgaccgg gattgcgggt cccgcgtgca cacctatcaa aagaaccgca
    48721 ttgcagccct tgccacgagc cttgccacgc gaagtggttc ggggactttt ccatccagcg
    48781 tgagatcccg gcagagccgg tttgcatcct ccatggggag gccgaacggg cggatataag
    48841 cggtgtgccc ggtagggagg ctgagtgggg ctctctcacc cagcgattcg taacgggcaa
    48901 ggcgggtcgc atctccggga aagtggcggc ggatatcatc cgaaagccca tccgagtcct
    48961 cgtaagtgac gaccacgacc gggactccgg tttccctgtg gatggatgca gggtcaagga
    49021 tattgaacca tgcaatcacg cacccgctca gcaggatgac attgatatcc tttcgatgaa
    49081 gttcccggta tatagccagt actccctccg tggcgtcgtt cccacccaca gtgatacgcg
    49141 agagggctgc tccatcgaca cgaaggtcct ttctcatcac cacacctgca agtatcgaat
    49201 actcgcgtga ggagaagctc tccgcgatac caagcacccg caggcctttc ttggcgatgt
    49261 gcatggaagc acttatgtca atggattttt aaatatagct cgatgcccat cgagaaggac
    49321 caggtctgcg tcctcatccc caccctgaac gagggtccta ccatcggggc cctggttcgc
    49381 gagttcaggg cgctcggcta tacccatatc tttgtcatcg acggaaacag cacagacgat
    49441 acccgcacca tcgcagccag agaaggtgca ggggtcgagg tgcagtcagg gaagggaaag
    49501 ggaaatgcac tcatcgaggc gtttccgctg atcgagcagc cctatatcct gatagtcgat
    49561 ggagacggaa catactcccc tgccgatgcg gagaagatgc tcgcaccgct ggccgagggg
    49621 tacgaccagg tgatcggtga caggttgacc gacaagaacc gggatgcatt ctcccggctg
    49681 aattattttg gaaaccaggt gctcaaccgc cttttcaagc tggctcactc agcctatctc
    49741 tcagatatcc tttcaggcta ccgggcattc acccgggacg cgatacagca gatgcatctt
    49801 catgaagcgg gctttgggat agagacagag atatcggctg aggcggtcag gagcgggcag
    49861 aagatctctg ttgttccaat cgaatacaaa aaaaggccgg ggacacccac caagctgaac
    49921 cctttccacg atgggttcaa gatcaccgcc accatctacc gcctggcacg gatgagcaac
    49981 ccgctcttct acttcggtct tatcggcctg atcctcatga ttgccggtgt cttcctcggc
    50041 atttacgtca tactcgagtg gctgcgtcag atagagcacc tgccgctgac aatcttcacg
    50101 gtccttctta tcgttatcgg tttccagata ttcatgtttg gcgtcctctc ggacatgatg
    50161 ctggccctcc accgcgaggt ggtgagagaa ctgcatgaga ctcgtgaaag aaaagaatcg
    50221 cgttgatccg aagggatttg gcatgagatg ctcccttcct cgatgatatc gcacattgct
    50281 ctctttcccc ctcttccact gaaaaaagaa cctttatctc ggacagacgc gaataatatc
    50341 taccatattt ctcgtgcgat agtagtctag cggcaggaca ggggcttccc aagcctctaa
    50401 cccgggttcg atccccggct atcgcatacc cgccatggat ga
//
LOCUS       DAOQYR010000004        49694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_786_length_49694_cov_8.574065, whole genome shotgun sequence.
ACCESSION   DAOQYR010000004 DAOQYR010000000
VERSION     DAOQYR010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 49694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49694
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_786_length_49694_cov_8.574065"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..1398)
                     /locus_tag="PKM87_00820"
     CDS             complement(73..1398)
                     /locus_tag="PKM87_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ28638.1"
                     /translation="MKRESIFCVFLAICLTFLLLSSGCTEQQPASQGGEVKIGVVASM
                     TGAASTTGKDIWQSAELAADEINAKGGIYVKELGKSVPIRLVQGDDESTREGGQKAVS
                     KLITQDNVDVLVGGYSSAVVSAHQSIVAEYKVPYIISGGSSTSVSHRDDIDTSYMFFH
                     RPNTDDSGEASILFVDEVVRPSILTKFGFSSDRPLKMAVIYQDSPFGKGQLDAVKNVI
                     KARNLPIEIVAEEPFKMGESDFRTVLTSVKAKNPDMIYSVTFLNELVPLTQQARRDVG
                     LNTLILAIENNDDPDYYKGVGTYGDGQVMESRFSPYAVPRGSTEEATRQFLANFDKKY
                     GGFAGMMGAATYESVYITKAAIEDAGSIDKAKVRDALATLSMPQMMEIMKGGTISFSP
                     DYRESKFDLYVVQLAMDQASGEPRPHIVWPENLKDKEFVLPDWYTPGSS"
     gene            1497..1628
                     /locus_tag="PKM87_00825"
     CDS             1497..1628
                     /locus_tag="PKM87_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28639.1"
                     /translation="MVQNSCCLHEPVAMAARILAGGIWSGGTRMSVALEREAMVVSG"
     gene            complement(1702..1986)
                     /locus_tag="PKM87_00830"
     CDS             complement(1702..1986)
                     /locus_tag="PKM87_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28640.1"
                     /translation="MNPKVRIIVEEFFPKIIETHIRTRSSIETARVSLERYRTMGLQV
                     IRNLPAGMKEEDLSFLEEAYRAALGRLEEFHGRESASSSSTVGQESSESL"
     gene            complement(2067..2639)
                     /locus_tag="PKM87_00835"
     CDS             complement(2067..2639)
                     /locus_tag="PKM87_00835"
                     /inference="COORDINATES: protein motif:HMM:NF021420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2121 domain-containing protein"
                     /protein_id="HNQ28641.1"
                     /translation="MSLVMAFSGTYSAVIAGDQREILFRGEASCIALLESELYSNRLC
                     TEEQLHARAAELDVSITIRDDKCKVWEQDGILIGEVTETDGPVIRKRRICVSCGRYAI
                     FEKSGESWSLLSRGEGSHFLVLGNEATKGIAHQCIREHWKPGGGTLEDAVKVLILSMQ
                     EAARRTASVSRTFSILQTRTKADVPCSLGM"
     gene            complement(2816..3562)
                     /locus_tag="PKM87_00840"
     CDS             complement(2816..3562)
                     /locus_tag="PKM87_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HNQ28642.1"
                     /translation="MPKCVKKDPVIQGSLCRSCPMPLPARIPYLDICPSVKVSLPERI
                     LRLVLPFILGGCYFAICLLSLPYETALLLGGLMLAYVIPPAGKESVIPLGIALGFPWW
                     LMACSIALMDVLAGIFMALNCDLALKIPVLGRWIQHFMERGGEFVNKRPWLGRFYFTG
                     VILFVMFPLQGSGGIGGTLVGRMIGLSPGSTLLAISVGAIIGCTVIALGSQAIKDLIF
                     MNLQMGIAVLFSVLAFLAIAYLIYRRRLRK"
     gene            3642..4322
                     /locus_tag="PKM87_00845"
     CDS             3642..4322
                     /locus_tag="PKM87_00845"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNQ28643.1"
                     /translation="MYIVGHRGARSPGPENTCKAVRYAMRCAEYVEVDIRLSRDRVPV
                     IMHDPTIDRTTNGSGRVADMTYGELSALDAGEGEHVPSLMEVCSLVRGICGLFVEIKE
                     PGSELEVCKILDKEAPSPLLVVSFHRASLEMVSEMLPGTATGFIYSRPVPDAPTMASR
                     KNIRVILPRSDLLTPELVADAQSRDIEVVPWTLDDLEQWEKAKKMGVDGFVSDNPCSA
                     RQWLEGAG"
     gene            4610..6388
                     /locus_tag="PKM87_00850"
     CDS             4610..6388
                     /locus_tag="PKM87_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNQ28644.1"
                     /translation="MDTSILLLGGVLAVTVILFVSDRVRIDLVAILASLSLAWLGLIS
                     PIEAFSGFASNAVIAMAAVMILGFGIERTGVTSRIANAIIRYAGTSEQRVVATTSMTV
                     GCLSSVLQNIGSAALFLPALRRIGKQTRIPVSRLMMPMGFAAILGGSITMIGSSPLIV
                     LNDLLRQSDAEPFSLFAVTPIGVPLLVAGVLLFAFAGDRILPKKEEKAGKTTVAEIWG
                     IDHPVWTAAIPPSSSLVGKTREEAFFKMRYGLDLLAVRIQREITVAPSRATRFEEGQE
                     LAFIGHEEEFKRFIRESGCIPSGPGSRFKEILDGEGYGFAELIVRPKASIIGRTMREI
                     RFRQLFSIEPIVHQRGEIETRADFSHMPLAAGDVIVAFGSWDTLRLLAGHKDLLLLTH
                     PEGDAVRHGMAHLAVLIFASSLALTMTGVPLSLALLTGSAAMVVSGVLTIDEAYRAIE
                     WKTIALIAGLIPLGIAMEKTGAAALVAGALIGSLGGAHPLVIMCAVAVLATVLSLVIS
                     NVAATVLLVPLVMIAGAGTGVDPRALALLVAVCASNSFILPTHQVNALLMGPGGYSTK
                     DFIKAGSVMTVIFIGVAVTLIYLLIA"
     gene            6405..7772
                     /locus_tag="PKM87_00855"
     CDS             6405..7772
                     /locus_tag="PKM87_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ28645.1"
                     /translation="MIIQQFFIPGIAHSSYIVAGNRTCAVVDPSRDVGRYISAAREMG
                     LRITHILETHLHADFVSGHLDLAEATGAEIYAPESGKCKFPHNALVEGDEIPLEHIHF
                     RVIETAGHTPEHVSYIATDTSRGDTPVALFCGDTLFVGDVGRPDLFPGKAEALASSLY
                     DNLHTKIMTLPDECEVYPAHGMGSLCGRAMAAKRTSTVGYEKKYNYALRISDRDEFVR
                     ALTSDMPAAPDHFARCSEINRSGPVLMRTLAQPAPVNPKAFSDWIRRDDSVLLDARSY
                     PAFSGVHIPGAWHIDLSGNFATQAGWILPPGQDILLVVESRNQAEEAALQLRRVGFDR
                     VPGFLEGGMLSWGSLNLRINRVPVISAEEAHSMVSSGEAVLVDARSKEEWEAGHADRS
                     MHIPWHDLRTRHAELDPARHTIVMCKGGQRASIAASILKMHGFATVSNLAGGYTSYQR
                     AGFAP"
     gene            7826..8359
                     /locus_tag="PKM87_00860"
     CDS             7826..8359
                     /locus_tag="PKM87_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNQ28646.1"
                     /translation="MFTWLAMATWSPYIVGAGIGVLVWVAFLVSDRPLGASTAYAKTA
                     GMVESALSRKKAAAMPYYRKFTPTVDWQWMIVVGIVIGAFLSAWISGTFSVFIVPPLF
                     AGAFSADPLLRIVVALIGGVLMGIGARWAGGCTSGHGISGTLQLSVASWVAVVCFFAG
                     GIFVAGLMFGFPFFSGV"
     gene            8363..8920
                     /locus_tag="PKM87_00865"
     CDS             8363..8920
                     /locus_tag="PKM87_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNQ28647.1"
                     /translation="MAFDTIRSNKSAQIVLGLLIGIAFGFLLQKGGVTEYAVIIGQLL
                     LTDFTVVKVMLAAILVGMIGIYAMKAAGLVRLHSKGGSVGATVIGGLIFGAGFAVLGY
                     CPGTAAGAIGSGALDAVVGVLGIVIGAGIFARLFPYLDRVILNRGAFPADTIPELLGV
                     RPALVVGIVAVLIVGVLAMLAMLGL"
     gene            9123..9548
                     /locus_tag="PKM87_00870"
     CDS             9123..9548
                     /locus_tag="PKM87_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNQ28648.1"
                     /translation="MAQSLRAGESRQPRKSVQIPLFYQVLVSMIFVAVIPVILLSVVS
                     MGGTASIVATIGTPATVLLLTIGTVLVVLLWSYFVAHRVTRPIVELSVVATRISRGYL
                     PEKEMEVQSHDEIGELIAAFNKMVNTYRILDTLAKEEPE"
     gene            9586..9933
                     /locus_tag="PKM87_00875"
     CDS             9586..9933
                     /locus_tag="PKM87_00875"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNQ28649.1"
                     /translation="MLKNELKRLFSLEGVKMVLVIDPEGRVHDEMGASNIEGEQLAAV
                     VSFVMAESRAMATRLGQEQISMVFVEFRELALVSVPLQEAYFLVVITHAGANIGQISY
                     ELKKYQDISSGYT"
     gene            9930..10805
                     /locus_tag="PKM87_00880"
     CDS             9930..10805
                     /locus_tag="PKM87_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28650.1"
                     /translation="MTGMLPAGFPVGEMQAPFKWMLIHAARFQGMIRVQAPDGEGHVL
                     VRKGTPMGCSFRRGSEDLKGEAAYQHILSFPVIDLSIHRYTTEEMEEAIRLLQASGSV
                     CGGGGAGPAYGSGGQPGETGPATGEERSPLPPAEADAPTAPVEKSRHTAAGTRETSPG
                     SLEEAPMVLPSGLDVENLAHLLLGRMLRLQGVQAVSIFGRGKSVLSIGDVELDSLVVL
                     AEDMLHAAKDISSVMRTGAFVHLTLQIPAGKVIIAPYFNEYICILTSPGVNLGQIRKI
                     LKEIPETGSPGRTVS"
     gene            10805..11464
                     /locus_tag="PKM87_00885"
     CDS             10805..11464
                     /locus_tag="PKM87_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ28651.1"
                     /translation="MYRILIVDDNPAITDILARMVRHEGLEAIEAHSGEEALAILGQD
                     HPDLIFLDILMEPMDGWETLDRIKRDKRTADIPVMMITAKSLDPAEVQAHTAHFEDYI
                     QKPVTRRELCRVIRQYFRKESEIIDEVEKARLSGMDAGLVDEFRRLSRDIEVHQRLLL
                     LLWNVYRTREDDDPQRPDLLEAIGRMEAAQNAMRSRHHEIRTRIRSQGKGCGSEGETG
                     G"
     gene            11501..11953
                     /locus_tag="PKM87_00890"
     CDS             11501..11953
                     /locus_tag="PKM87_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation endopeptidase HyCI"
                     /protein_id="HNQ28652.1"
                     /translation="MKNLLLGIGNPLRKDDGAGNYVAKSFRKAGWQVIDCGTAPENFS
                     GTVRRHHPDLLVLVDAADMGLAPGEFAIIPKEKIRDVGIGTHQLPLDIFIDFLGDAPG
                     ATLVIGIQPLRIETGEGLTPAVREGVDRLIGILEKGKVGSIPVLTGES"
     gene            complement(12088..12492)
                     /locus_tag="PKM87_00895"
     CDS             complement(12088..12492)
                     /locus_tag="PKM87_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNQ28653.1"
                     /translation="MKFLPLFPELVRQLFREPATNRFPAKYLPPTVTGFLKAVGEGKA
                     TIVPPVPTPPNFRGKITYKNDCCIGCGLCVKVCPSHAIELIPETKQIRVWVGQCIFCS
                     QCTSICPKGGLSMGDEFLLADEDRYSENLIVE"
     gene            complement(12489..13508)
                     /locus_tag="PKM87_00900"
     CDS             complement(12489..13508)
                     /locus_tag="PKM87_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNQ28654.1"
                     /translation="MSPVDLAVMLSWFVLLSLFALAAGLFLLGVDRKLAAHMQARIGP
                     PIRQPFIDVAKLLQKENVVPANAISAVFHLSPLIALASSIVILFYLPVGSLLPVLGMY
                     GDLVLVMYLLTVPALAMVAGGFASGSPYATVGAQREMVTMIAYEFPLAVVVIGVAWRL
                     GAAGLSSPFALGTMAANPIWTVVGPLGIAGILLLLLVLAVVTPAELSRIPFDTQEAET
                     ELAGGLLVEYSGRNLAMFYLAQGVKTLVLAAVIVAIFFPWNLSGFLAMPPWLALPADI
                     LFFLVKAILVLFFSVTLVRVGMARLRINQVVSVYWIYLSVIGLTGLLLLMADAALPTL
                     MGVGA"
     gene            complement(13505..14734)
                     /locus_tag="PKM87_00905"
     CDS             complement(13505..14734)
                     /locus_tag="PKM87_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNQ28655.1"
                     /translation="MSDESKKAPYIVPIGPNHPALKEPVMFTFEMEGEMVRDVDFAPG
                     QTHRGIEWMGMRRNPVQIVHLTDRICGICGVSHTFAFARAVEQIAGIDVPERADYIRT
                     IIAEFERIQSHLLWAGVAAHELGFDSLLHLTWRVREQSMDVIENLTGNRVNYGIVQVG
                     GVRRDIVPEQHQVIEDCLTTYEGLLGKLLNLFLDDKTIRMRCRDCGVLSKKEALELCT
                     VGPTARASGVNVDLRQDYPYAAYGDLDVKAILPDQHIGETHGDVYDRIVVRLLEVGQS
                     CQIIRQCLSEMPSGPVTAEEKLAKLLAVCKKATGEAIGRVEAPRGECFHYVRMEGKEA
                     PHTWKVKASSYSNLMSWIPMLRGEQIADIPIIVASIDPCLSCTDRVAVVRGGQQSILT
                     KEDLHRLSVDKTRRLQR"
     gene            complement(14731..15381)
                     /locus_tag="PKM87_00910"
     CDS             complement(14731..15381)
                     /locus_tag="PKM87_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNQ28656.1"
                     /translation="MTHAFLTPEQVVERFTTKFGASIKGTRISERREGKRKEPGYDIW
                     IDLERDLLKPAISTLMDLDYPHLSVISGSDMGDSVRLLYHMTIYYGRAKGEYTVTFSV
                     LLPKGDLTIPTISDLIPGAVFSEREKQEFLGVKVSGIPDGRRLFLPEDFPEGIYPWRK
                     DGTGIPDGMVKDLWASKRPTDRPAPPLPEAQACEMETEEAPPSPAGREPGKEGGQS"
     gene            complement(15378..15812)
                     /locus_tag="PKM87_00915"
     CDS             complement(15378..15812)
                     /locus_tag="PKM87_00915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNQ28657.1"
                     /translation="MKLNTAFNRSLWVYHFNSGSCNGCEIEIVATITPRYDPERFGIK
                     LVGSPRHADVMLVTGPVVHKMRDRLLRVYAQMPEPKLVMCIGACGISGGVFYDSYNLE
                     GPVDEVIPVDVYVPGCPPRPEAIIHGVVKALQKLERIQGGES"
     gene            complement(15809..16156)
                     /locus_tag="PKM87_00920"
     CDS             complement(15809..16156)
                     /locus_tag="PKM87_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HNQ28658.1"
                     /translation="MDLTSTLLTGAGSWNPLPWVLGMVVAAIIAWLIYSMGEKGYKAG
                     TAQAEPFISGNPEPEKGMVHVRAGNLYWGYLEALKGYYDRLVPLHTGDLHDYVLWYLG
                     ITALFLVLVVISA"
     gene            complement(16161..17735)
                     /locus_tag="PKM87_00925"
     CDS             complement(16161..17735)
                     /locus_tag="PKM87_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNQ28659.1"
                     /translation="MIPSFIVENSPALMVIVPLVAAFITPFIGRISSGIRSLWVGGAM
                     AVTSAITLILAFDVYSAGTRLYVFGARAASAAIPPDSGGIPIRIIFEVDPMSALMLVI
                     TAVSGFAIVLYSLSSERSHTGLESYYTLYLLMITGILGMVSTGDIFNFFVFLEILSLA
                     SAGLIAYRIDGGVAVEAALKYFVLSTIGALAILVAIGIYYGEYNALNMAMIAARMQFS
                     LLDQVALVLIIAALAMKCGAVPMHFWTPDAYSMAPSSITALLVVASQASLYGLFRTVF
                     SLYNVTLNAVTVGWLIIILGVLSMVVGVTMAIPQKNVKRLMAYHAVSQTGYMLLGVGV
                     GLAVLGNQSLLDAFGYMAMEGGIFHIINHAMYKGLLFLTAGAIFLRIGTYNLNRMGGL
                     GHSMKWTMVFFVIGALAIAGIPPFNGFASKLMIYESVFVFNPLLAVIAMVVSILTLAS
                     FVKVFHSIFMGPKLPEFANVKEAPMPMLLGMGILALVVVLFGLFPGIVVESLITPAAH
                     ALVDQGAYISAVLGGA"
     gene            complement(17732..18076)
                     /locus_tag="PKM87_00930"
     CDS             complement(17732..18076)
                     /locus_tag="PKM87_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HNQ28660.1"
                     /translation="MFWNLPLIAVVVLTIIALAIIVFKKNLIKVFLGIVILESAVNLF
                     LISLGYREGGIAPIYTNAPSTAMVLPTPQALTLTSIVIGVATSALLLSFAIIIWKKYG
                     TIDIDRIRRLKE"
     gene            complement(18079..18546)
                     /locus_tag="PKM87_00935"
     CDS             complement(18079..18546)
                     /locus_tag="PKM87_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HNQ28661.1"
                     /translation="MRMSKIVRTGANLLYPFILIFGLYVIMHGHLTPGGGFQGGAVVA
                     TAAALVIVAYSYDEVTGMLKKTAMKARESIGLLLFIGAGIAALAVGAPFFYNFLANSG
                     LLFGMSVPAGPNPGDLNTAGVIPIMNIAVGIEVWGGLAVILLYMLSGIRKEES"
     gene            complement(18543..18818)
                     /locus_tag="PKM87_00940"
     CDS             complement(18543..18818)
                     /locus_tag="PKM87_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28662.1"
                     /translation="MRKILTAIIFVVLATGLCAIPAFLAFGEPAASEMDQYFIGHGQS
                     EAAANNIVTSIVFDYRGFDTLGESVVLFTAVVGVGLMFRSMKGGKEQ"
     gene            complement(18815..19069)
                     /locus_tag="PKM87_00945"
     CDS             complement(18815..19069)
                     /locus_tag="PKM87_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HNQ28663.1"
                     /translation="MIDLVIHALVIAGLVASALMVFHFRDLLAAALAAGFFSFLISLE
                     FYILQAPDVAISQAAIGAGLTTAIFIIAIRATTRYEGGQP"
     gene            complement(19066..19422)
                     /gene="mnhG"
                     /locus_tag="PKM87_00950"
     CDS             complement(19066..19422)
                     /gene="mnhG"
                     /locus_tag="PKM87_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HNQ28664.1"
                     /translation="MIADVLILACLAVGIFFNALGIAGLLRFPDVYTRMHATTKMTTF
                     GSMFTALSVIIYGVALFLSSGDGQYVTLSVHTLIAAISLAFTNAVGSHAIARGAHKAG
                     IKPAPAIVDRLEEFTP"
     gene            complement(19419..19676)
                     /locus_tag="PKM87_00955"
     CDS             complement(19419..19676)
                     /locus_tag="PKM87_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HNQ28665.1"
                     /translation="MIDPWYIAVCILVILVCLSLIRLFLGPTAADRAVAIDTVNTLSV
                     AVLILLGVAYQQIIFIDVAIVYALLSFVSTLYIAKYLGGEL"
     gene            complement(19673..20200)
                     /locus_tag="PKM87_00960"
     CDS             complement(19673..20200)
                     /locus_tag="PKM87_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HNQ28666.1"
                     /translation="MIPFLLTFILALLLYLLLAAGSGSVLLWSPSELVAGVALSLLAA
                     AVARNLLCRSDSYRMANPLRLLLIPLYLLGPFLLEVTKANLDVAYRVITGNIRPGIIR
                     VQSGLRTDLGIFMLANSITLTPGTITVDIDEKSHDLYVHNINIPQGWEERPQVEEREL
                     FSLANLPAWIRRIAE"
     gene            20532..21941
                     /locus_tag="PKM87_00965"
     CDS             20532..21941
                     /locus_tag="PKM87_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HNQ28667.1"
                     /translation="MRSPSLRRRMLWWCDSCHTPVLANVCSRCSTRAREVMITPPADA
                     RPAFPSDISHVNKVYSEHFGVPLIPPGHIVLLNKVPDPDRMEEIIVGGGVVGSIKYLP
                     GESRWEAIPRPQASVFLTPRRKFVVVDNGAVPSIQQEGASVLAPGLVEIEPSVSRGDE
                     VFVLDEERAVVAVGRAKVDAKEAGNMERGAIVRTRRNFPSNCTRGEATWEDAVEASAG
                     VLEEQEAEAISFIRDVAARNPLPVNVSYSGGKDSLATLLLVNKALGKVPMLFADTGLE
                     FPETCRNVEEVSREFGNPVVRARGEEAFWKAFQVHGPPAVDFRWCCRTAKLNPVEKVI
                     REHWGECLSFIGQRKYESFRRMASGRVWRNPHLKSQLSAAPIHHWTALQVWLYLFREN
                     APYNPLYESGLDRVGCFMCPSSDVAVLRQIEESHPLLWQRWSSRLEEWQKEKGLPDSW
                     RGEARWRMKGVPVEEDNNC"
     gene            21919..22527
                     /gene="hisH"
                     /locus_tag="PKM87_00970"
     CDS             21919..22527
                     /gene="hisH"
                     /locus_tag="PKM87_00970"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNQ28668.1"
                     /translation="MKKIIIVDYGLGNLRSVRRGIEKSGGVPVISTDIHEISSAEGIV
                     LPGVGAFRDGMQQLGDLQEVLVDISHSVPVLGICLGMQMLMEWSEEHGVHRGLGLVPG
                     VVRRFPRREGYKIPHMGWNTLSLTAPENPLFEGIQSGEFMYFVHSYYADTQAVYTMAA
                     TEYVCPFSSVIWNRDRVFGVQFHPEKSGAAGLQLLRNFIAMV"
     gene            complement(22590..23105)
                     /locus_tag="PKM87_00975"
     CDS             complement(22590..23105)
                     /locus_tag="PKM87_00975"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNQ28669.1"
                     /translation="MNKTFGCLLSGILVLMLCTCGCSTPGSPGRSPPAGTAPPVVSIP
                     GGEAGLEGVMWYLVVFHTASGRSAEPVQGTEITAFFDGKGDISGSSGCNHYRGTYSAK
                     EGEVSIRDIAATEKYCISPSGVMSQEAIYMAFLQRVSGYQTENGLLTLTDNKGSPLLT
                     FGMATPRSLKQ"
     gene            23404..24327
                     /locus_tag="PKM87_00980"
     CDS             23404..24327
                     /locus_tag="PKM87_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28670.1"
                     /translation="MKSVTRLDDFLGPLHQGIWEITVGKDLVTEPLDSGWERSLINVP
                     TPGTIASFRKGQYHVHETSTEWKVHLDRYDPKVHPFLHLVDDAPLLLMVADTFVTLVA
                     TARGTHGNYMDAELKEQKRTWQVLFLLGFALALVGIWIFADPLTTFSWIIHVLLPAGI
                     IVLGLFVARKGISFMRRAIVSPGSFFLGISVVVLGIVTFDLPLDAFVQLLLLVLSFWA
                     FGSAYMSFSRVARGRKAVPEGFFRRLGTGVLSFLLALGILLVPDAMLELLMDIFGILV
                     FMLGIVISTSGWRLREKMRSSRANPANEAGR"
     gene            24470..25096
                     /locus_tag="PKM87_00985"
     CDS             24470..25096
                     /locus_tag="PKM87_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28671.1"
                     /translation="MHARVRSGLRIMKEISKALIFFFLFVIAVPLIIGVIQEVPAGNI
                     LSFLASTFLLQAAAPPLGGPLGLSQMVVLAVMASFAIGMVLAILEICESLALTSERVS
                     GWIEKVGKKMERYPAIQKYGAISCTLIAWIPGIGLYGTPIIAWILGWNRWLVVVFTTA
                     GFVIAAAFVLFVAQHIHSIEDVFILGVAGAAGVIALILAGKYARKRAS"
     gene            complement(25131..25700)
                     /locus_tag="PKM87_00990"
     CDS             complement(25131..25700)
                     /locus_tag="PKM87_00990"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HNQ28672.1"
                     /translation="MADTVILVTPCDRGWSALVILGLLSILFGVLMILFPDLTAIAVV
                     TLVGVLVIILGVIMLLSSLFLPSGATRSTLLLFGGLIGLLIGVGIIVYPIIAGAIMTE
                     IIGAAIFIFGMMQVLFGLVFEEGKRRALYFISGILSLIFALLIIFYPFIGSIILFGYL
                     LAIYFLLMGILMVLAGYLSHCACARFSES"
     gene            complement(25863..26174)
                     /locus_tag="PKM87_00995"
     CDS             complement(25863..26174)
                     /locus_tag="PKM87_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28673.1"
                     /translation="MKLWKSRSSGNKRRESEKEDLVSRLKKDVPMMRGQGEPPDKDPA
                     HFSGFLCDLCNSPFAYHELRQCILCGRWACSSCWTPEFYVCNSCNGMITLHTMGDKSS
                     R"
     gene            complement(26143..26481)
                     /locus_tag="PKM87_01000"
     CDS             complement(26143..26481)
                     /locus_tag="PKM87_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28674.1"
                     /translation="MTVTEALLIFLLIILIGFIIYYYFRGSSGKLEITRPMESRLDEY
                     LDRRFDSMIDEWSLLRKPQAQEFRDEKLADLSRDEKRMASLTEFERQFGEQMANLEER
                     LETLEKQVIR"
     gene            complement(26478..26699)
                     /locus_tag="PKM87_01005"
     CDS             complement(26478..26699)
                     /locus_tag="PKM87_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28675.1"
                     /translation="MMGLDYMERYIDRRMKDLIEDWNLATRNDLGDLYSRLHAIEDEA
                     RMCAGFEATATAKMTELEKRLEHLKGAKR"
     gene            complement(26696..27004)
                     /locus_tag="PKM87_01010"
     CDS             complement(26696..27004)
                     /locus_tag="PKM87_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28676.1"
                     /translation="MTVYETPGAIGEKHSFIDSIKDIPGYIDQRLPTMDKDLDAYFDQ
                     NLPAIIEEWGLLGSVHLKELERRLSRVHQQIDFLEKGRTSLEARAAKLEKELKKLEGL
                     "
     gene            complement(27011..27394)
                     /locus_tag="PKM87_01015"
     CDS             complement(27011..27394)
                     /locus_tag="PKM87_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28677.1"
                     /translation="MADIWVTVNQSFPLLLIILGLIIVYFIIREVRIMYTKTRIAKMD
                     LEKEKLQLIKADLEQKGHPFFRVSPAKQEELRSLDEENTMLETDIFAQQSAVEKRIQR
                     LESKVKLTKLDHMIEKIKKEEDRLG"
     gene            complement(27411..27878)
                     /locus_tag="PKM87_01020"
     CDS             complement(27411..27878)
                     /locus_tag="PKM87_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28678.1"
                     /translation="MHKSYLSQLGIKEGDDVEISLGEEAKPVVVAVFADTLVEEGHIR
                     LSPEDIAAIGAAPGSSVIVTRRPPLADRVKTGAETTAEKVKGGAAQAAEKIRGGAPEA
                     AGKVKESATQAAEKVKEGASQAAEKVKEGASVAKESIEKSTETVKKKLQDKDL"
     gene            28161..28961
                     /locus_tag="PKM87_01025"
     CDS             28161..28961
                     /locus_tag="PKM87_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28679.1"
                     /translation="MRSAVFFLLGLLVLAGSVNAYVLTISAPSEIRAGAPLEVVGNTT
                     FPVGTQFDLVIYRLQPTTPAEVTRTIIIVDESKSFQASFPTTGMEAGDYKVEARFLTD
                     PGSKLGSDSVTMRVFRITDRSGEIVLTVPTDQVLGEALRVEGYIPKAGVTTITLKVSG
                     PQGAVLPPQDIRTTTRTGRDEGYFMKMFPVSERGNYYVDFYDVKGFMTTVKFMVNAPP
                     PGTEEVVTPGATASPTESAIPTRTPFPVAGCCAALIGAGLIARRVRRI"
     gene            complement(29208..29849)
                     /locus_tag="PKM87_01030"
     CDS             complement(29208..29849)
                     /locus_tag="PKM87_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ28680.1"
                     /translation="MTDTLHDDAGDRGAGAPAGEVVVLQPGDERAQKIGKAIASQAAN
                     DILHALQERPKTAGDLASTLAMPMGTVKYHIENLLDAGLLEIRETRYSVKGREIKVYG
                     LKDQLLIVAPRVQNIRSLLLKYATLFGIIILASVAAYAMLPILSAPPGMGGEDVAKFQ
                     GERSVGIMAEPAPSAVAPVQGLALDPVLAFFLGGCLVIILLIIYEVAVWRRLK"
     gene            complement(29871..32612)
                     /gene="alaS"
                     /locus_tag="PKM87_01035"
     CDS             complement(29871..32612)
                     /gene="alaS"
                     /locus_tag="PKM87_01035"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNQ28681.1"
                     /translation="MLEEEYQLEYFKTHGLTRKICKKCGSAFWTRNPDTEICGDAPCE
                     TYNFIGAPIFRRHTVDEMREAYLSFFEKNGHTRINRYPVAARWRDDIYLTIASIADFQ
                     PWVTGGVVPPPANPLTISQPCIRLNDLDSVGRSGRHLTLFEMMAHHAFNSDVEEIYWK
                     ERTLELCEQFISSIGGDLSRVTYKEHPWIGGGNAGPSLEVLIGGLEVATLVFMNLGRQ
                     KTGKPGIDLAGEMYYPMSNWIVDTGYGLERFVWASRGSPTIYDAVFPEMVSRVMEAAG
                     LAHALEDKEYTKILSLNAKFAGVMDISGTNLYQLRKKVATAIDVPIERLDKMITPIEK
                     VYAIVDHTRCLAYMLGDCIVPSNVREGYLVRLVLRRTLRMMNDLGIKEPLADLIEQQM
                     RIVGTSSFEQDTLIVREIVEREVEKSNATMERGTKIVQRIARSYKNKRERVPLKEVIT
                     LYDSHGIPPEIVREVALAEGAVVDLPDNFYSLIADMHSESGPVAEEDPFARYRDRISA
                     LPATRKLFYEQPCDMEFDAMVIDFFDGYVVMDQTLFYPEGGGQPPDCGMLMTAENMVR
                     VDDVKKVGDVIVHHVTGGTLRRGDRVKGIVDEERRWSLMRHHTATHLLLHASKEVLGA
                     HIHQAGAQKGLESSRLDIRHYKHITPDELKKIEIAANRMVMADQVVDVKHLDRTRAEQ
                     LYGFALYQGGVPPGREIRIVQVAGDVEACAGTHCRSTGDIGFVKVIRVEHIQDGIERL
                     EFAAGLAAVEYVHRIESLVAQAADVLSVQNENLPATVSRFFSEWKGQKKEIERLRQNL
                     VELEIKNLTLETVNSTPLLVRQIDLSGKELAAIGGEISGKGGVALLANRSEPATVLLM
                     SGTEKVNAGEIIGQVCSLLGGKGGGSRTMAQGGGPEVHQVDLALKVGRERILEALRG"
     gene            complement(32675..32998)
                     /locus_tag="PKM87_01040"
     CDS             complement(32675..32998)
                     /locus_tag="PKM87_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HNQ28682.1"
                     /translation="MDPEEFWRLLKKEGVLTPRLSDEFVRIYGDRGQKALHALKNGMV
                     RKYLDFVVVAGTSQSYVVEEDFCTCRDFVFRGKCCWHLLAARLASICGGYAEREEWYQ
                     DTWGI"
     gene            complement(32968..33696)
                     /locus_tag="PKM87_01045"
     CDS             complement(32968..33696)
                     /locus_tag="PKM87_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNQ28683.1"
                     /translation="MDIGEFTRIMEALAPPDLAEEWDDGKIGLVVEGVENLDHICCAL
                     DATIATVGKAVSEGADMLVVHHTPLWTPVTAFRGSIAALLRRAVASGMNIYVLHTNYD
                     HAPGGVNDILAGLLDLKDARPLSLGVVGECRLGLQEIADRLCCPLRAWGSPRLPGMLA
                     VVGGSGFSPDLIAEAAEESADSLLSAELRHSVARGSPIPLVEATHYALEAPAMKALAQ
                     KMGWAYIEDPPLLHTWIPKSSGAC"
     gene            33787..35295
                     /locus_tag="PKM87_01050"
     CDS             33787..35295
                     /locus_tag="PKM87_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HNQ28684.1"
                     /translation="MKKTIHQINERIRDGNARVVTAEEMPAIVAELGEEGALAEVDVV
                     TTGTFGAMCSSGGFFNFGHADPPIRMERVWLNDVEAYAGLAAVDAYIGATQQSTTRED
                     QYGGAHVIEDFIAGKSVELRAISRGTDCYPRRTITTDLLLENLNQAIMCNPRNGYQRY
                     NAASNSTERVLHTYMGMLLPSFGNVTYSGAGLLNPISNDPGFRFIGSGVPCFLGGAEG
                     MVIGEGTQASPAAGFGTLMVTGDMKKMNTEYIRAATMHGYGVTMYVGLGIPIPVLDVE
                     TVRSTAVKDEDIWVDIIDYGVPARDRPTIGKVNYAMLKAGHLEINGEEVRTSSLSSLR
                     RARRVAEELKGWIESGKMAVCLPTRRIDPGRRAAPMRETVQGPRVLEIMDRRVITISE
                     NEPIRDAARKLLKGETNHLPVINGEGVLVGIVTTYDVSKAVVNPAKISHVKDIMRRKV
                     VTTTADEPVDIAVRKLEKHNISALPVVDREYRVVGMLTAMDLGKLFGGRWLK"
     gene            35292..35684
                     /locus_tag="PKM87_01055"
     CDS             35292..35684
                     /locus_tag="PKM87_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ28685.1"
                     /translation="MKLLVTFSRKEGKKPIIAQVVRDTGVLVNVERALIDSSEGEALI
                     DIPDESCRIVKDKLADLGASVRILEEEISLDETECVDCGACISICPQEVFSFDEEFRL
                     RMDAKRCVLCGRCIEICPHGALSRRVDI"
     gene            complement(35811..37097)
                     /locus_tag="PKM87_01060"
     CDS             complement(35811..37097)
                     /locus_tag="PKM87_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ28686.1"
                     /translation="MPHQRPAGHRRRRTGHYSPPVYLVTVQVGSQWTRECMARLATIF
                     GLLPPERHAPHITLFGPFTLDKGCDIRVLLEDPLLRSPGFSSFSAMLGGALVLRGRKG
                     YAAVIRAAPGDPLALLAAAVRDSLLPHTRTCTWIDQIAGQRIFHVSTGFGLRRRKAEE
                     IVEFLDTLPPGRRNAEGMRCMAGTTLDLFRLEVIRKGTLMDAFDFPTGTWIGRPAAFS
                     EDRWEKTLESFRQKSGYQIDHPSFSEEDTAFVISDLHLGHANIITYTSRPFPDAATMD
                     SVLIQNWNFRVRPTDTVYFLGDLAYGRNAGPAARYLSLLAGDVHIVAGNHDSGLGHAS
                     GSMEVTWRSRRFLMVHDPAEAPPDYPGFVVHGHLHNNQPGEYPFLNMPGRRVNVSAEM
                     VGYVPLSLDELVDIIETSPGDAQFPTLNDARRKLNR"
     gene            37201..37911
                     /locus_tag="PKM87_01065"
     CDS             37201..37911
                     /locus_tag="PKM87_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HNQ28687.1"
                     /translation="MIRRRFHFRETIATILAEEESHVQAAREGLMQARQELEHYIAFD
                     PFFRTTMEPYEAVSDSTVVKRMAAAGASAGVGPMAAVAGAIAWAGVESMARTGAEFAV
                     IDNGGDIALLSDRVLLVGVHAGRSPHSDRFAFQVTPRAEVLGICTSSATVGHSLSLGI
                     ADAVTVFSRDPGLADAWATAICNAISPDDTSIIDKVPWGEVEGVFVVAGEWASSFGSL
                     PHMVRARVDHDLVTGGPW"
     gene            38037..38651
                     /locus_tag="PKM87_01070"
     CDS             38037..38651
                     /locus_tag="PKM87_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ28688.1"
                     /translation="MEGAMDRTILVVDDNRRLVDLLRTILEMEGYIVHAAYSGDECLA
                     YLDRERPGLILLDIVMSPMDGWTVLERIRERPGTGKTRVIMLTARHPTRKEAEKYAAL
                     IDGYILKPFDVRNLLESIRRAFAEQERLEGVIRSAHEKGTKRSFLAEYCRLSRIVNAQ
                     EALRALLSPGPGQGEGEEESDEARKLATMKQIIQQVVGIPGLDE"
     gene            complement(38730..39140)
                     /locus_tag="PKM87_01075"
     CDS             complement(38730..39140)
                     /locus_tag="PKM87_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28689.1"
                     /translation="MQDREKVPSYGELAGIAEHLLEKADEDEALLARELDTVDPAVRR
                     ELLVSDFLNAYQVYYYYFREAPGELEKERLILQPASALVQGVMIAELELLEVIFRVED
                     DQPVMSVSDGDQVLVNYRGRDAYRRALAFIDASL"
     gene            39355..39645
                     /locus_tag="PKM87_01080"
     CDS             39355..39645
                     /locus_tag="PKM87_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNQ28690.1"
                     /translation="MREEELDWQVYHLLMDDAGRDEDALAALLHCTPGEVHTSIGRLE
                     KAMLLECTPGGVRVLSVQEMALRCQARYDRSCPFSIKGGVIRLKGGSDEKDD"
     gene            39638..40162
                     /locus_tag="PKM87_01085"
     CDS             39638..40162
                     /locus_tag="PKM87_01085"
                     /EC_number="2.1.1.206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(56)-2'-O)-methyltransferase"
                     /protein_id="HNQ28691.1"
                     /translation="MTEIFVLRIGHRPERDQRVTTHVGLTARALGARGMYLAARDSGV
                     VTSIRDVVSRWGGDFLVQDQVSWKKCIRDWKAAGGIVVHLTMYGEGIRSHEEALQRCH
                     RILVVVGAEKVPGEVYGLADYNISVTGQPHSEIAGLAVFLDRFFKGTELEREFPGAKI
                     RVVPSPEGKITEEQ"
     gene            40159..41235
                     /locus_tag="PKM87_01090"
     CDS             40159..41235
                     /locus_tag="PKM87_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNQ28692.1"
                     /translation="MKGAVLVCGFATRHVARSAHAAGYTVYAVDHFCDQDLSWYTADR
                     ERFENLSDLPGAVENICSRHRVDWLVPTSGAETLPGQIPVLGTPPGRAARFMDKLLTQ
                     EFFESLQVPVPKILGPGCYPAMVKPRAGSGGWRNMVVHSDAELACWLETFECPPYLSQ
                     EVVTGTPASVCCVADGKGNARAIAANYQILREKAGAQFGFSGSVTPLVHPQAQQMAEM
                     AEKIASASGCTGTFGIDFVIGDEPWAIEVNPRFQATLDTVEAATGLNLFSAHVRACQG
                     EIPPGRPSPVGFAVRRILFADRDMVVRNDLSFLAPAVADIPWPGSFFEEGHAVISVYG
                     WGRNGEAAMKLLDTHMKRVRQYMG"
     gene            41243..41755
                     /locus_tag="PKM87_01095"
     CDS             41243..41755
                     /locus_tag="PKM87_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HNQ28693.1"
                     /translation="MEEILANTPIRAYLARLIGEEGISLLESFPREGEHSDEELAAQT
                     GINLNSVRHTLYTLYEKRLAEYRRIKNNETGWLTYLWHLRIDRVYDVIAEDMEAILEK
                     LEAREKFEEENDFYICKSCGFMFTFSQAMDCQFQCPSCETGLEHFDNEILLRALKRRI
                     RSMKESLGHA"
     gene            41781..42254
                     /locus_tag="PKM87_01100"
     CDS             41781..42254
                     /locus_tag="PKM87_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNQ28694.1"
                     /translation="MRSSGCSKRVIAHCRSVRDLAIEYLHDDIMDREVLEAGALLHDI
                     GRGITHGLPHAQAGADLCRRRGVPEEVARVVERHIGAGLTADECTLLGLLPIDCMPRT
                     LEERVVAHADNRVAGTRRICLDERLLHAIHLQKRQKQRLYRLWQEMEMFRQTPGT"
     gene            complement(42232..42744)
                     /locus_tag="PKM87_01105"
     CDS             complement(42232..42744)
                     /locus_tag="PKM87_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator of amino acid metabolism, contains ACT
                     domain protein"
                     /protein_id="HNQ28695.1"
                     /translation="MWSEIIGEFGDSPSQTRVLRFLLENGFGVNADGRITCNGIEIPA
                     TQVAKALDTDRRVVDTTAQRILSLPLHRNIFMHMRAAPDLSRVAGHLGLSVMTILPKD
                     ASERGIVSAAVRVIAEEGLSIRQIYVTDPVLSEEPRLVVIIEGDFPPAVIEGIRRLPQ
                     VRQITFQGSA"
     gene            42912..42996
                     /locus_tag="PKM87_01110"
     tRNA            42912..42996
                     /locus_tag="PKM87_01110"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42948..42950,aa:Leu,seq:gag)
     gene            43148..43444
                     /locus_tag="PKM87_01115"
     CDS             43148..43444
                     /locus_tag="PKM87_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28696.1"
                     /translation="MTGIISHGTDGVTMGDFDPKKHIAEEAGYDAKDNYVRGLKMPET
                     RFKNMVHNHPPDEQKSMRTQVDQAEAAGLRNYQVKHSKGFYIIENGIVVGSGRG"
     gene            complement(43641..44561)
                     /locus_tag="PKM87_01120"
     CDS             complement(43641..44561)
                     /locus_tag="PKM87_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28697.1"
                     /translation="MKGFRILMLLLLGCCAILAFPATAAETTVKSVLYQSDFSESPGW
                     NTNSPTRYYWDPSLEMYHFKTEGGTNGYAFIPVKYNGQSFIIDFDVVILTSQKESGFR
                     FGLISSEMDFTRGTNVLSSFENGKYGKIMSLRVIDQNNQLREATSYYTSYCGNLPGCQ
                     TVEFKENMTYHVTARYTKELQHADIKVTEKESGELVWGYFVSVGRDLFFMDRLAITTR
                     GDYAFGPFLEGYIDNVELVVFNPVETTPTTAVPMQVPTDVTTIPTTTVPTTPTPTPTP
                     STPLGDSLPLAAMGIAGSLLGNSIRRKRLK"
     gene            44905..48210
                     /locus_tag="PKM87_01125"
     CDS             44905..48210
                     /locus_tag="PKM87_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ28698.1"
                     /translation="MDGQIYYNRGVAFHKKGMFDEAISDYSRSIEINPHDAEAHHNRG
                     VARAGKRDYYGAIEDYNSAIRLNPHFTEAYYNRGVAYSLMGRPEEAIAEFSTALETDP
                     DFIEAYYNRGLEYSRQGEDELAIADFTRALELDPAYVAAYNNRGVLFGRKGMHDEAIG
                     DYSKACEIDPNFIEALFNRGIEFSRAGRLQEAVADYTRALEIDPGNPDILYNRGIALG
                     KMGQSEPAIADYLACLEKNPGSAEAYNNLGVEYSRLNLYDTSLEHYAKAIALNPHFVE
                     AYNNRGVLFNRLGRYDEAIAEFSRAIDIFDQAASEVHDETKTVSHPPAPAVPEPVKEK
                     HPEDSHVIGEPSPGVRDEAPMDPAILELSREIDHAPDDPELYNRRGLEYSRLASYEQA
                     IEDFTRAIEIDSGYIEAYYNRGNEYDRLGRIEEAIADYTRALKINPQYAEACYKRGDE
                     YQKAGLSDKAISEYTKALEINPNYPDALYRRGREFSRIGLSDMAIRDFSRALEARPGY
                     VEVLISRGEEYGRKGLSEKAIADYTSVISHNPSQKETYHKRGMEYEKSGKVDEAIADF
                     TGALERDPLWADAYLQRAHAYWLAGKYEESVQDSTKACEIDHENAEAFLQRGMSQSAM
                     DNSSEAIADFTRAIEVDPRCHEAYFRRGQAYSNSGRSEEAVGDFASAIHLNENVRETY
                     IARAAEFGQSGRNGEAISDLTRAIELSPDDDSTYLARGLLYDRQGMVDEAIADFSRAI
                     EINPGNEMAYNNRGNAYAARGLFNEAISDFSRAIERNPRNATSFYNRGIEYGENGMLD
                     QAIADLSEAITLRPDLGEAYYNRGVAFHKKGMLDEAIRDYERALEFQADNEKAYNNRG
                     NAYAGLGLYDEAIADFSRAIAINPGNPTAYYNRGIEYGRKGLIDQAMEDFSRAIEIKP
                     DLAEAYYNRGVAYQRVERLKEAIADYTRTIDLAPGNEKAWNNRGNAHAGLGMYNEAIS
                     DFTRAIEINPENPTAYYNRGIEFGKKGLSDEAIADFSRAVELKPDLAEARYNMGNIYY
                     NRNQCEEAIREFDRALSIQRGYARAYLNKALACEKLSRIPEARSAYEGFLRYARQESK
                     ELVDFVSQRLRDL"
     gene            complement(48295..>49694)
                     /locus_tag="PKM87_01130"
     CDS             complement(48295..>49694)
                     /locus_tag="PKM87_01130"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ28699.1"
                     /translation="NIERSGNIVYLYHDGGDPLQKGRTIVRINGNDIPESSIAFLHAQ
                     DWPWTPGKTMRIQYDGPGIPALVQVIYSGGSGEMLVFSDEAAAVQETPIPVSTVPTAV
                     TTTPPGATATPTGAGAVTSPVSPAATGTTPSAPQPPQAAFTADPRAGQLPLAVQFTDT
                     SAGTPTSWLWNFGDGETSDQKNPVHTYKNPGMYSVSLTVRNQYGTSQKTETGFISAGT
                     LPVAQFTALPAEGTLPLIVHFTDLSTGLPTAYAWDFGDKATSNEKNPLHTYTKAGTYD
                     VTLTVGNEYGYNTRIQTGAVQVKEPERVDIYLTGSRMGTLSTGYLQFLVTGEGGWIKI
                     AGSTYPFSPGDTVQLFVDQSQSGMIDVNARGIAGFRFDNVRMFVNGNPARSGISTDIF
                     VPSFAGLQSTLAITIPPGDATTVLFINNGRIIPGSAQQVIISNLAENSQGQMSLGVKL
                     GSVEYRGGAGSYTVS"
BASE COUNT        10388 a        13986 c        14741 g        10579 t
ORIGIN      
        1 gaaggcaacc tggctgatta tcccgatggt cccgtccata cagtaatcct tctcctgtgc
       61 ctaaaaaaag atttaggagg atcctggggt gtaccagtca ggcaggacaa attctttgtc
      121 ttttaggttt tcgggccaga cgatgtgggg ccggggttct ccagacgcct ggtccattgc
      181 aagctgaacg acgtagagat cgaacttgct ctcccggtaa tccggtgaga aggagatggt
      241 gccacccttc atgatctcca tcatctgtgg catggaaagt gttgcgagtg catcccttac
      301 ctttgccttg tcgatgctcc cggcatcctc gatggctgct tttgtgatat atactgactc
      361 gtaggtcgcg gcacccatca tccctgcaaa cccgccatat tttttgtcga aattggcgag
      421 gaactgcctt gtggcctctt cagtcgagcc ccttggcact gcgtacggcg agaacctgct
      481 ctccatcacc tggccgtcgc catatgtgcc cacgcccttg tagtaatcgg ggtcgtcatt
      541 gttctcgatg gcaaggatca gtgtgttgag accgacatct ctccttgcct gctgggtcag
      601 gggcacgagt tcgttcaaga atgtcacgga gtagatcatg tcaggattct ttgccttcac
      661 ggaggtcaat actgtcctga agtccgactc ccccatcttg aacggttctt ccgcaacgat
      721 ctcgattggg aggttcctgg ccttgatcac gttcttcacc gcatcaagct gccccttccc
      781 gaacgggctg tcctggtaga tgacggccat cttcagtgga cggtccgagg agaaaccgaa
      841 cttggtgagt atggacggcc tgacaacttc atcgacgaac agtatgctcg cctcgccgga
      901 atcgtctgtg ttcgggcggt ggaagaacat gtagctggta tcgatatcat cgcggtgcga
      961 gacggatgtg ctcgaacctc ccgatatgat atatggcacc ttgtactctg cgacaataga
     1021 ctggtgggca gagaccacgg cactcgaata gcccccaacg aggacatcca cgttatcctg
     1081 ggttatcagc ttggagaccg ctttctggcc gccctcgcgg gtggattcgt catctccctg
     1141 tacaaggcgt atcggaaccg atttcccgag ttccttcacg tatatgccgc ccttcgcgtt
     1201 gatctcatcc gcggccagtt ctgcggactg ccagatatct ttgccggtcg tgctggccgc
     1261 accggtcatg gaagcgacca cccctatctt gacctccccg ccctgactgg cgggctgctg
     1321 ctctgtgcag ccggaactca acaacaggaa cgtcaggcag atagccagaa atacacagaa
     1381 aatactctcg cgcttcatgt ggactcacca tgctatgata tagcatgaca tacaaaaact
     1441 ggggcggggt agttaatagg atttccgatg ttgttgtgag aatccagggg agtgtgatgg
     1501 tgcagaattc ctgctgtctg catgaacctg tcgcaatggc tgcacgaatc ctggcgggcg
     1561 ggatatggtc aggagggaca cggatgagtg tagccctcga aagagaggca atggtcgttt
     1621 caggatagac aaagccatgt gaacgcgaat tcagcgggaa aatatgcagg tcagggagaa
     1681 aagagaggat gaacctgccc tttataggga ctcggagctc tcctgtccaa ccgtcgagga
     1741 tgacgatgca gactccctgc catggaactc ctcgagccgc ccgagtgccg cccggtaggc
     1801 ttcctcgagg aaggataagt cctcctcctt cattcctgca gggaggttcc ggatgacctg
     1861 cagccccatt gtccgatagc gttccaggct gaccctggca gtctcgatcg aagacctggt
     1921 tcgtatgtgt gtctcgatga tcttggggaa gaactcttcg acgattatcc gcacttttgg
     1981 attcattctc tctctccctc tcttccactc acctctttcc tgtcccctta tcaaggtctc
     2041 tttcagggaa ggggtgcggg tgcgtgttac ataccaaggg agcaaggcac atccgccttt
     2101 gtccttgtct ggaggatgga aaaagtccgg ctcactgatg ccgtcctcct tgccgcctcc
     2161 tgcatggaga ggatgagcac tttaacggca tcctcgagcg tgcctccgcc tggtttccag
     2221 tgctccctta tacactggtg agcgataccc ttcgtggcct cgtttccaag gaccaggaaa
     2281 tggcttccct ccccccggga aaggaggctc caggactccc cggacttctc gaagatagcg
     2341 tacctgccgc aggagacgca gatgcgcctc ttcctgatca cggggccgtc cgtctcggtc
     2401 acctcgccga tgaggatccc gtcctgctcc cataccttgc acttgtcgtc tctgattgtg
     2461 atggacacgt caagctcagc cgcccgggca tggagctgtt cttcggtgca aagcctgttg
     2521 gaatagagtt ctgattccag gagcgcgatg caggacgcct cccccctgaa aaggatctcc
     2581 ctctggtccc ctgcaatcac cgcgctgtat gtccccgaaa atgccatgac aaggctcatc
     2641 gctgttcacc tgttggagga tgggacatgt cgatcagtca ggtctcccct ggattgaatg
     2701 cttcggctgc atggtttccg gatgacgaga ggctgatgtt ttttttttct tgccatagca
     2761 gccccgggaa ttgtatccca cgcacgccgg gtgattgtga cctgaacccc ttccctcatt
     2821 tcctcaatct cctccggtag atgaggtatg cgattgccag gaacgccagc actgaaaaga
     2881 gcactgcaat ccccatctgc aggttcatga agatgaggtc ttttattgcc tgggagccaa
     2941 gcgcgatcac ggtgcatcca atgatcgccc caacggagat ggcaaggagt gtagagcccg
     3001 gtgagagccc tatcatcctt cccacaaggg ttcccccgat tcctccgctc ccctgcaggg
     3061 ggaacatgac gaagaggatg actccggtga agtagaaacg cccgagccat ggcctcttgt
     3121 tcacgaattc accgcctctc tccatgaaat gctgtatcca tctccccagc acggggatct
     3181 tcaaagcaag gtcacagttc agggccatga atatccccgc gagcacatcc ataagggcaa
     3241 tcgagcatgc catgagccac catgggaacc ctagtgcaat gccaagggga atgacgctct
     3301 cttttcctgc gggggggatc acgtatgcaa gcatgaggcc gcccaggaga agcgcggttt
     3361 catacgggag ggagaggaga cagatcgcga aataacagcc cccaaggatg aacgggagga
     3421 caagacgaag gatccgttca gggagggaga ccttcacaga ggggcagata tccaggtaag
     3481 ggatgcgggc ggggagtggc attgggcatg aacgacacag ggacccttgg atgaccgggt
     3541 ccttcttcac acattttggc atggggacac attatggttc ttccgccaca gtcgcgtgcc
     3601 agcagcttat ttttaattgg ggatacatat ccccgggatc gatgtacatc gtaggccaca
     3661 ggggcgcccg gtcaccgggc ccggaaaata cctgcaaggc ggtccgttat gcaatgaggt
     3721 gcgcggagta cgtggaggtg gacatccggc tctcccgcga cagggtcccg gtgatcatgc
     3781 atgacccgac tatcgaccgt accacgaacg ggagtggccg tgttgcggat atgacatatg
     3841 gagaactgag cgctcttgac gccggagaag gggagcatgt cccctcgctc atggaagtct
     3901 gttcacttgt cagggggatt tgtgggcttt tcgtggagat caaggagccg ggaagcgagc
     3961 tggaggtatg caaaatcctg gacaaggagg caccttctcc tctcctggtg gtctcattcc
     4021 accgtgcatc tctcgagatg gtctccgaga tgctcccagg gaccgcgacc ggcttcattt
     4081 attcacgccc cgttcccgac gccccgacaa tggcatccag aaagaatatc cgggtaatcc
     4141 ttccgaggtc tgacctcctc accccggaac ttgttgctga tgcacagagc agggatatcg
     4201 aggtggtccc ctggactctt gatgacctgg aacagtggga gaaggcgaaa aagatgggtg
     4261 tggacgggtt cgtatcggac aatccatgca gtgcaaggca gtggctggaa ggggccgggt
     4321 gaggaggacc cacacatcca tttttcctct gaactgacag ggtgggctga atcgggatcc
     4381 gcgatatact gcaaatgatc cccggacaat tgtccaaacc catgctgatt atccccatcc
     4441 ttgaatcccg gacggatagc ggtggactct gcgattgcag tatcaagtgg tgccgggctc
     4501 gtattatgag tcgcgagggg atgcaaggtc ccggccgaaa agacaaccag cgcatcggcg
     4561 ttcttgggta atctttttcc tctcttcggc cacatctgcc tataacgcca tggataccag
     4621 cattctcctc cttgggggag tccttgcggt cactgtcatc ctctttgtga gcgaccgggt
     4681 ccggatcgac ctggttgcca tcctggcaag cctttcgctg gcctggctcg gcctcatatc
     4741 cccaatagag gcgttctctg gattcgcgtc gaatgcggtc atcgcgatgg cggcagtgat
     4801 gatactcggg ttcgggatag agcggacggg tgttacgtcc cgtattgcca atgcaatcat
     4861 caggtacgcc gggacaagcg agcagcgggt cgttgccacc acttcgatga cagtcgggtg
     4921 tctctcttcg gtcctgcaga acataggatc cgccgcactc ttcctgcctg cattgaggcg
     4981 catcggaaaa cagacccgga ttcccgtctc ccgtttgatg atgccgatgg ggtttgccgc
     5041 gatactcggg gggagcatca cgatgattgg gtccagcccc ctgatcgtgt tgaacgatct
     5101 cctccgtcaa tctgatgcag agccgttctc cctctttgcg gtcacgccga taggggtccc
     5161 cctccttgtt gcaggggtgc tgctctttgc attcgccggg gatcgtattc tccccaaaaa
     5221 ggaagagaag gccgggaaaa cgaccgttgc agagatttgg gggatcgatc acccggtctg
     5281 gacagcggcc atccctccgt cgtcatccct tgtgggaaag acccgggaag aggcgttttt
     5341 caagatgcgg tacggtcttg accttctcgc cgttcgtatc caacgggaga tcactgttgc
     5401 cccctcgcgt gcgacacggt tcgaggaagg ccaggaactt gcattcatcg ggcatgagga
     5461 ggagttcaaa aggttcataa gggaatccgg gtgcataccc tcagggcccg gaagcaggtt
     5521 caaagagatc ctcgatggcg aagggtatgg atttgcggag ttgattgtca ggccgaaggc
     5581 ctccatcatc ggcaggacca tgcgcgagat caggttcagg cagttgttct cgatcgagcc
     5641 tatcgttcac cagagggggg agatcgagac acgcgctgat ttttcgcata tgccccttgc
     5701 agcgggagat gtaatcgtgg cgttcgggtc gtgggacaca ctcaggctcc ttgcagggca
     5761 caaggatctc ctgctcctca cccatcccga aggtgacgca gtgcggcatg gcatggcgca
     5821 cctcgcggta ctgatatttg cctcgtccct tgccctcacc atgaccggag tccccctgtc
     5881 acttgccctt ctcaccggtt cggcggcgat ggtcgtttca ggggtgctga caatcgacga
     5941 ggcgtaccgg gcaatcgaat ggaagacaat cgccctgatt gccggcctga tccctcttgg
     6001 tattgcgatg gagaagaccg gtgcggcagc gctcgtcgcg ggggccctca tcgggagtct
     6061 cggcggtgca catccccttg ttatcatgtg tgccgtggca gtccttgcga cagtgctctc
     6121 ccttgtcatc tccaatgttg ccgcaactgt cctgctcgtc ccgctggtga tgattgccgg
     6181 ggccgggacc ggcgtggatc cccgcgccct tgccctcctg gttgcagtat gcgcatcgaa
     6241 ctcattcatt ctccccaccc atcaggtcaa tgcactcctc atgggtccgg gagggtattc
     6301 gacaaaggat ttcatcaaag ctggaagtgt catgacggtg atcttcatcg gagtcgccgt
     6361 aacactcatc tatctcctca tcgcatagac cggtgaggag cgtaatgata atccagcagt
     6421 ttttcattcc cggcattgcc cacagttctt acatcgttgc gggaaacagg acgtgtgcgg
     6481 ttgtcgatcc atcgcgcgac gtgggtcgct acatctctgc tgcacgcgag atggggcttc
     6541 gaatcaccca tatcctggag actcacctgc atgcagactt cgtatccggt caccttgacc
     6601 ttgccgaggc aaccggcgca gagatatatg cgccagagtc cggcaaatgt aaatttcctc
     6661 ataatgcact cgttgaaggt gacgagatac cccttgagca catccacttc agggtgatcg
     6721 agaccgcagg ccacactcct gagcatgtct cgtatatcgc aaccgatacc tcccgcggtg
     6781 atacccctgt cgcccttttc tgcggcgata ctctctttgt cggcgatgtc ggccgtcctg
     6841 acctctttcc cgggaaggcg gaagcgcttg cgtcctcgct ctacgacaac ctccatacca
     6901 agatcatgac cctccccgat gagtgcgagg tctatcccgc ccacgggatg gggtccttat
     6961 gcggaagggc gatggcagcc aagaggacaa gtaccgtggg ctacgagaag aagtacaatt
     7021 acgcactccg gataagtgac agggatgagt ttgtccgggc ccttacttcg gacatgcctg
     7081 ctgcccccga tcacttcgca cgatgttctg agatcaaccg gtcgggcccg gtcctgatgc
     7141 ggacactcgc gcaacctgcg cctgtcaacc cgaaagcctt ctccgattgg atccgcaggg
     7201 atgactcggt ccttcttgat gcccggagtt acccggcatt ctcgggagtg cacattcccg
     7261 gtgcctggca tatcgacctt tccggaaact ttgccacgca ggcgggctgg attctcccgc
     7321 cagggcaaga cattctcctg gtcgtcgagt ccaggaacca ggcggaagaa gcggcgcttc
     7381 agctccgccg cgtcggattt gaccgggtgc cggggttcct ggagggcgga atgctttcat
     7441 gggggtcatt gaacctccgg ataaaccgcg tgccggtcat atcggcagag gaggctcatt
     7501 ccatggtcag ttcaggagag gcggtgctcg ttgatgccag gtcaaaggag gagtgggagg
     7561 ccggccatgc tgatcggtcc atgcatatcc cctggcacga cctcaggaca cggcatgccg
     7621 agcttgaccc tgccaggcat accatcgtga tgtgcaaggg aggacagagg gcgagcatcg
     7681 cggcaagcat cctgaaaatg catggctttg cgacggtctc taacctcgcg ggcggataca
     7741 cctcgtatca gcgggcgggt tttgcgccct gatgggagcc attatgacag aagaacccaa
     7801 tcctccctga taccggagat cattcatgtt cacatggctt gcgatggcga cctggtcgcc
     7861 ctacatcgtc ggtgcgggga ttggagttct cgtgtgggtg gcgttcctcg tctcggaccg
     7921 cccgctcggt gcatcgaccg catacgcaaa gactgccggg atggtggaat cggcactctc
     7981 aagaaaaaag gcagcggcaa tgccgtacta caggaaattc acaccgaccg ttgactggca
     8041 gtggatgatt gttgtcggta tagtgatagg agcgttcctc tctgcctgga tatcagggac
     8101 tttttccgtt tttattgttc cacccctctt tgcgggagca ttttctgcgg atccgctcct
     8161 gcgcattgtc gtggcgctga tcgggggggt cctgatgggg atcggtgcaa ggtgggccgg
     8221 tggctgcacg tcaggtcacg ggatatccgg gacgcttcag ctctcggtcg cgagctgggt
     8281 cgcggtggtc tgcttctttg caggcgggat cttcgtagcc ggcctgatgt ttgggttccc
     8341 gttcttttcg ggggtctgaa ccatggcatt tgacacgatc aggtcgaata aaagtgccca
     8401 gatcgttctc gggctcctca tcggcatcgc gtttggcttt cttctccaga aaggcggggt
     8461 gacagagtat gcggtgataa tcggccagct cctcctcacc gatttcaccg tcgtgaaggt
     8521 gatgcttgcc gcgattctcg ttgggatgat tgggatctat gccatgaagg ctgcgggcct
     8581 tgtccggctt cattccaagg gtggttcggt tggtgcgacg gtgatcgggg gattgatctt
     8641 cggtgcaggg tttgcggttc tcggctactg tcccggcact gctgcaggtg cgatcggatc
     8701 gggtgccctg gatgcggtgg tgggggtcct ggggatcgtg atcggcgccg gaatcttcgc
     8761 ccggctcttc ccgtaccttg accgggtgat actgaaccgg ggtgcgtttc ctgcagatac
     8821 catccccgaa ctgctcgggg tccgcccggc gctggtggtc ggaatcgtgg cggtcctcat
     8881 tgtcggggtg ctcgccatgc ttgccatgct gggtctctga ttcccgctct ttcttttaca
     8941 gcgaacgaga caggggattt gggaacccgg gttttctcct gatctctgca cacgggtgtt
     9001 acaccttcct ttatggattg gtgagtttat tcgagccatc ccaatcgttc aaaccgcaga
     9061 aatcgcgcgg aggcaccccc aacagaaaag gtttaatgcg acccggggcg gatctcttct
     9121 gcatggcgca gtctttgcgg gcaggggaaa gcagacagcc cagaaagagt gtgcagatac
     9181 ccctctttta ccaggtgctg gtcagcatga tcttcgtggc agtgataccg gtaatcctcc
     9241 tctcggtggt ctccatgggg ggcactgcca gcatcgtggc caccattggc actccagcta
     9301 ccgtccttct cctcaccata ggcaccgttc ttgtggtcct cctgtggagc tacttcgtgg
     9361 cgcaccgtgt cacaaggccg atcgtagaac tttcagttgt cgccaccagg atcagcaggg
     9421 gatatctgcc ggaaaaggag atggaggtcc aatcccacga tgagatcggg gaactcattg
     9481 ccgcattcaa taagatggta aatacctacc ggatactgga tacgctggcg aaggaagagc
     9541 cggagtgaac ttctgtcaac caggtgcaag gagaaaacga ctgcaatgct gaagaacgaa
     9601 ctcaagcgcc tcttctccct cgaaggagtg aaaatggtgc tcgtcatcga ccccgaaggc
     9661 agggtacatg acgagatggg ggcatccaat atcgagggtg aacagcttgc agcggtggtg
     9721 tccttcgtga tggccgaatc cagggcgatg gcgacgcgcc ttggccagga acagatctcc
     9781 atggtgttcg tggagttccg ggaacttgcc cttgtatccg ttcccctcca ggaggcatat
     9841 ttccttgtcg tgattaccca tgcaggggcg aatatcggcc agatcagcta tgaattgaag
     9901 aagtaccagg atatctcgag tgggtacaca tgaccggaat gctacccgcc gggtttccgg
     9961 tcggagagat gcaggcgccg ttcaagtgga tgctcatcca cgccgcacgc ttccagggga
    10021 tgatccgcgt gcaggcacct gatggggagg ggcatgtcct tgtcagaaag ggcaccccga
    10081 tgggctgttc cttccggagg gggagcgagg atctgaaagg agaggctgca taccagcaca
    10141 tactcagctt tcccgtcatc gacctctcca tccacaggta taccaccgaa gagatggaag
    10201 aggccattcg gctgctccag gcatctggtt cggtttgtgg agggggaggc gcgggacctg
    10261 catacggatc cggtggtcaa cccggggaaa caggacctgc gacaggggaa gagcgttctc
    10321 ccctgccccc tgcagaagct gatgcgccga ctgcgccggt cgaaaagagc aggcataccg
    10381 ccgccggcac gagggagacc tctcccgggt ctctcgagga ggccccaatg gtcctcccca
    10441 gcggcctgga tgtcgagaac ctcgcccacc tgctcctggg gcgcatgctc aggctccagg
    10501 gggtccaggc ggtctcgatc ttcggcaggg gcaagtcggt gctctccata ggggacgtgg
    10561 aactcgactc cctggtggtt ctcgcagagg acatgctcca tgctgcgaaa gatataagct
    10621 ccgtcatgcg taccggcgca ttcgtccacc tgaccctgca gatacctgcc gggaaggtaa
    10681 tcatcgcacc ctatttcaac gagtatatct gcatcctcac cagtcccggg gtcaacctcg
    10741 gccagatacg aaagatctta aaagagatcc cggaaaccgg gagcccgggg aggaccgttt
    10801 cctgatgtac cgtattctca tcgtggacga caacccggcc attaccgata tccttgcaag
    10861 gatggtgagg cacgaggggt tggaggcgat cgaggcccat tccggagagg aggcgcttgc
    10921 catccttggc caggatcacc ctgacctcat cttcctcgac atcctgatgg aaccaatgga
    10981 cgggtgggag actcttgaca ggatcaagcg ggacaaacgg actgccgata tcccggtgat
    11041 gatgatcacc gcaaaaagcc tcgaccccgc agaggtccag gctcacaccg cgcacttcga
    11101 ggactatatc cagaagcctg tcaccaggcg ggaactctgc agggttatca ggcagtattt
    11161 caggaaagag tcggagatta tcgacgaggt ggagaaggcc cgcctgtccg gtatggatgc
    11221 cggtctcgtc gacgagttcc ggagactctc gagggatata gaagttcacc agagactttt
    11281 gctcctgctc tggaacgttt accggacaag ggaagatgac gatccgcaga ggcctgatct
    11341 cctcgaggcc atcgggcgca tggaggcggc tcagaatgcc atgaggagtc gtcatcatga
    11401 gatcaggacc cggatcaggt cccaggggaa agggtgcgga agcgaaggcg agaccggtgg
    11461 ctaggggcat tggtcacagc cgcatcgtgg acctgattgc atgaagaacc tcctccttgg
    11521 catcggcaac cctctccgga aggatgacgg tgcagggaat tacgtggcaa aatctttccg
    11581 gaaagcgggc tggcaagtga tcgactgcgg gactgccccc gaaaatttct cgggaacagt
    11641 gaggcgccac cacccggatc ttctcgtcct cgtggacgcc gcggacatgg gtctcgcccc
    11701 gggggagttt gcgatcatcc cgaaggagaa gatcagggat gtggggattg gtacccacca
    11761 gctcccgctc gatatcttca tcgactttct cggggatgcc ccgggtgcca ctctcgtgat
    11821 tggaatccag cctctgcgga tagagacggg ggaaggcctc actcccgcgg tcagggaagg
    11881 tgtggaccgg ctcattggaa tcctcgagaa aggaaaggtc gggagtattc cggtgcttac
    11941 cggggagtcc tgattgtcgt ttcgggtggg tgcaggtttc ccggatgtgg ggtagcacca
    12001 ccccatcact atcaggcgac tttggagctt acctgcctgc aattccagga ccgcgcagtc
    12061 ccgggtgaaa aagggacgaa cccttcctca ctccacgatc aggttctccg agtaccgatc
    12121 ctcgtcggca aggaggaact catcacccat ggagaggccg cccttcgggc agatactggt
    12181 gcactgggag cagaagatgc attgcccgac ccagacccgt atctgttttg tttcggggat
    12241 gagctctatt gcgtgggagg ggcagacctt cacgcacagt ccgcacccga tgcagcaatc
    12301 gtttttgtag gtgatcttgc cccggaagtt cgggggggtc gggaccggcg gaacgattgt
    12361 ggcctttccc tctcccacag ccttcaggaa acctgtcacc gttggcggga gatacttggc
    12421 cgggaagcgg ttggtcgcag gctcgcggaa gagctggcgg acgagttcgg ggaacagggg
    12481 caggaatttc atgcccccac ccccatgagc gtgggaagcg cggcatctgc catcaggaga
    12541 aggaggcctg taagcccgat gactgaaagg tagatccaat agaccgagac tacctggttg
    12601 atgcgtaacc ttgccattcc cacccggacg agggtgaccg agaagaacag caccaggatt
    12661 gctttcacga ggaagaacag gatatccgcg ggcagcgcca gccacggggg catcgcgagg
    12721 aatcccgaga ggttccacgg gaaaaagatt gcaacgatca cggccgcgag cacgagtgtc
    12781 tttaccccct gggcaaggta gaacatggca aggttcctgc ccgagtactc caccagcagg
    12841 cctcctgcaa gttccgtctc ggcttcctgg gtgtcgaatg ggatccggga gagctcggcc
    12901 ggggtgacga ccgcgagcac caggaggaga aggaggattc ctgcgattcc cagtggcccg
    12961 accacggtcc atatggggtt tgctgccatg gtcccgagcg caaacggcga ggagagaccg
    13021 gccgccccaa ggcgccatgc gacaccgata acgacaaccg cgagcgggaa ctcgtaggcg
    13081 atcattgtca ccatctcgcg ctgggccccg accgttgcat atggagatcc tgacgcaaaa
    13141 ccccctgcga ccatcgcaag tgcgggcacg gtcaggagat acatcaccag caccaggtct
    13201 ccgtacatgc cgagcaccgg caggaggctt cccaccggca ggtagaagag gatgacgatg
    13261 cttgatgcca gcgcgatcaa cggtgataag tggaagacgg ccgaaatggc gtttgcaggg
    13321 accacgttct ctttttgcag aagcttggcg acgtcgataa acggctgtcg gatcggcggg
    13381 ccgatcctgg cctgcatgtg ggccgcaagt ttccggtcca cgccaagaag gaagaggccg
    13441 gccgccagcg caaaaaggga gagcagcaca aaccagctta gcatgaccgc aaggtcgaca
    13501 ggactcatcg ctgcagcctc ctggtcttat ccacggacag gcgatggaga tcctccttcg
    13561 tgaggatgct ctgctgcccg cccctgacca ccgcaacccg gtcggtacag gagaggcagg
    13621 gatcgattga ggcaacgatg atggggatat cggcgatctg ctcgccccgc agcatcggga
    13681 tccaggacat caggttgctg tacgaggatg ccttcacctt ccaggtgtgc ggggcttcct
    13741 ttccttccat tctgacgtag tggaagcact ccccgcgcgg ggcctcgacc ctcccgattg
    13801 cttcgccggt tgccttcttg cagacggcaa ggagttttgc gagtttctcc tcggcagtga
    13861 ccggcccgga aggcatctct gagaggcact gccggattat ctggcaggac tggccaactt
    13921 cgagcagcct taccactatc cggtcgtaga cgtcgccgtg agtctcgcca atgtgctggt
    13981 cagggaggat ggccttcaca tcgaggtcgc cgtatgcagc atatggataa tcctggcgga
    14041 gatccacgtt gactcccgag gcccgcgcag tcggccccac agtgcagagt tcgagcgcct
    14101 ctttctttga gagcaccccg cagtcccggc accgcatcct gattgtttta tcgtcaagga
    14161 agaggttcag aagttttcca agcagcccct cgtacgtggt gaggcagtcc tcaatgacct
    14221 ggtgctgctc cgggacgatg tcccggcgca cgcccccgac ctgcacaatg ccgtagttca
    14281 cccggtttcc agtcaggttc tcaatgacgt ccatcgactg ctcccgcacc ctccaggtga
    14341 ggtgaagaag cgagtcaaag ccgagctcgt gggcggcaac gcccgcccac aggaggtgcg
    14401 actggatacg ctcgaactcg gcaatgatgg tgcggatata atccgcccgc tcggggacgt
    14461 cgatcccggc aatctgctcg actgcgcggg cgaatgcgaa cgtgtgggag accccgcaga
    14521 tgccgcatat ccggtcggtg aggtggacga tctggacggg attccggcgc atccccatcc
    14581 actcaatacc ccggtgcgtc tgcccggggg caaagtccac gtccctgacc atctccccct
    14641 ccatctcgaa ggtgaacatc accggctcct tcaatgcagg gtggtttggc ccgatgggga
    14701 cgatgtaggg tgctttcttc gactcgtcgc tcatgattga ccgccctcct ttccgggctc
    14761 ccggcctgcg ggcgagggag gggcctcttc ggtctccatt tcacatgcct gtgcctctgg
    14821 aaggggtggt gccggcctgt ctgtaggcct cttcgaggcc cagaggtcct ttaccatgcc
    14881 atccggtatt cctgtgccgt cttttctcca ggggtagatg ccctcgggga agtcctctgg
    14941 gaggaaaagc cgccggccat cggggatgcc ggagaccttc acaccgagga actcctgctt
    15001 ctcccgttcg gaaaagactg ctccgggaat caggtcggag atcgtcggga tggtgaggtc
    15061 gccctttgga aggagcacgg agaacgtgac ggtgtactcc ccttttgccc tgccgtagta
    15121 aatggtcata tggtagagga gacggacgga atctcccatg tcgctcccgg agatgaccga
    15181 gaggtgcggg taatcaaggt ccatcagcgt gctgatggca ggtttcagga gatcccgctc
    15241 aaggtcgatc catatgtcgt atccgggctc ctttctcttc ccttccctcc tctcgcttat
    15301 ccgggtgccc ttgattgaag caccgaactt cgtggtgaac cgctccacca cctgctcggg
    15361 tgtcaggaac gcgtgggtca tgactctcct ccctggattc gctcaagttt ctgcagcgct
    15421 ttcaccaccc cgtggatgat tgcctccggc cgcgggggac accctggcac atagacgtcg
    15481 acagggatca cctcgtcgac aggcccctcg aggttgtatg aatcatagaa gactccccct
    15541 gagatcccgc aggccccgat gcacatcacg agtttgggct ccggcatctg cgcatatacc
    15601 cggagcagcc ggtccctcat cttgtggacc acaggcccgg tgacgagcat cacgtcggca
    15661 tgccgcgggc tccccaccag ctttatgccg aaccgctccg ggtcgtaccg tggggtgatg
    15721 gtcgccacta tctcgatctc gcagccgttg caggaacccg agttgaagtg gtagacccag
    15781 agggaccggt tgaatgcagt gttcagcttc atgcggagat caccaccagc acaaggaaaa
    15841 gcgccgttat tcccaggtac cacaggacat agtcatggag gtcgccggtg tggaggggaa
    15901 cgaggcggtc atagtaccct ttgagcgcct cgaggtatcc ccagtagagg ttccctgcac
    15961 ggacatggac catgcccttc tcgggctccg ggttcccgga gataaacggt tcggcctgcg
    16021 cagttcccgc cttgtaccct ttctctccca tcgaatagat cagccaggcg attatggcgg
    16081 ccaccaccat gccgaggacc cagggaaggg ggttccagga acctgccccc gtgaggagag
    16141 tggatgtcag gtccattgtc tcatgcacct ccgaggaccg cgctgatata cgcgccctgg
    16201 tccaccaggg catgggccgc tggcgtgatc aggctctcca ctacgatgcc cgggaagagg
    16261 ccaaagagca ccaccaccag tgcaaggatg cccatcccga ggagcatcgg catcggcgcc
    16321 tccttcacat tggcgaactc cgggagtttc ggacccatga agatggaatg gaagaccttc
    16381 acgaaggatg ccagggtcag gatgctcacc accatcgcga tcactgccag gagcggattg
    16441 aagacaaaga ctgattcgta aatcataagc ttggacgcaa acccgttgaa gggcggaatc
    16501 cctgcaattg ccagcgcgcc aatcacgaag aagaccatcg tccatttcat cgagtggccg
    16561 aggccgccca tcctgttcag gttgtatgtg ccgatgcgga ggaatatcgc acccgcggtg
    16621 aggaagagga gccccttgta catggcatgg ttgatgatgt ggaagatgcc cccctccatg
    16681 gccatgtacc cgaacgcgtc gaggagcgac tggttcccaa ggaccgcaag acccacgccg
    16741 accccaagga gcatgtaccc tgtctgtgag actgcatggt aggccatcag gcgcttcacg
    16801 ttcttctggg ggattgccat cgtcactccc accaccatcg agaggacccc gaggattatt
    16861 atcagccacc ccaccgtcac cgcgttcagg gtgacgttgt agagggaaaa gacggtcctg
    16921 aagagcccat agaggcttgc ctggcttgcg accaccagga gtgcggtgat ggaggaaggt
    16981 gccatcgagt acgcgtcggg ggtccagaaa tgcatcggga ccgcgccgca cttcatcgcg
    17041 agcgcggcaa tgatcagcac cagcgccacc tggtcaagga gggagaactg catcctcgcg
    17101 gcgatcatgg ccatgttcag ggcattatac tccccgtagt agatcccaat cgcgacgagg
    17161 attgcaagtg ccccaatcgt gctcaggacg aagtacttga gggccgcctc caccgccact
    17221 ccgccgtcta tccggtatgc gatgagcccc gcggacgcaa gcgagaggat ctcgaggaag
    17281 acgaagaagt tgaagatatc ccccgtggag accatcccga ggatcccggt gatcataagg
    17341 aggtagagtg tgtagtagct ctcaaggccc gtgtgggacc gctcgctcga gagggagtag
    17401 agtacaatgg caaagcccga taccgcggta atcacgagca tcagggcgct catcgggtcg
    17461 acctcgaata tgatcctgat ggggatgccg cccgagtcgg gggggattgc cgccgatgcg
    17521 gctcttgccc cgaagacata caacctggtc cctgccgagt acacatcgaa tgccaggatg
    17581 agggtgatgg cggatgtgac ggccatggca ccgcccaccc agagcgagcg gatccctgac
    17641 gatatgcgac cgatgaacgg ggtgatgaag gccgcaacga ggggcacgat aaccatcagg
    17701 gccggggagt tctccacaat gaaggagggg atcattcctt cagcctccgg atcctgtcaa
    17761 tatcgatggt cccgtacttc ttccatatga ttatggcaaa cgagaggagc agggcactcg
    17821 tggccacccc gatcacgatc gaggtcaggg tgagggcctg gggggtggga agcaccattg
    17881 cagtcgatgg tgcgttcgtg tagatgggtg cgatccctcc ctcgcggtac ccgagcgata
    17941 tgaggaagag attgactgcg gactcgagga taacgatccc aaggaagacc ttgataaggt
    18001 ttttcttgaa cacgatgatt gcaagtgcaa tgatggtcag aacgaccact gcgatgaggg
    18061 gaaggttcca gaacatggtc aggactcctc cttcctgata ccggagagca tgtagaggag
    18121 gatgaccgca agacctcccc agacctcgat ccccactgcg atattcatga tggggatgac
    18181 tccagcggtg ttcagatcgc cgggattcgg gccggccggg actgacattc cgaagaggag
    18241 gccgctgttt gcaaggaagt tatagaagaa cggagcgccc accgcaagtg cggcgatccc
    18301 ggcaccaatg aagagcagga gcccgatcga ctcgcgggcc ttcatcgccg tcttcttcag
    18361 catgcctgtc acttcatcgt aggaataggc aacgatgacc agggctgcgg cggtcgccac
    18421 caccgcaccc ccctggaacc ccccgccagg cgtgagatgt ccgtgcatga tcacgtagag
    18481 gccgaagatg aggatgaagg gatagagaag gttggcccca gttctcacga tcttgctcat
    18541 cctcattgtt ctttccctcc tttcattgaa cggaacatca gccccacccc caccaccgcg
    18601 gtgaagagga cgacagactc cccgagcgta tcgaatcccc tgtagtcgaa cacgatcgag
    18661 gtgacgatgt tgtttgccgc ggcctccgac tgcccgtgcc caatgaaata ctggtccatc
    18721 tcggacgcag cgggctcgcc gaatgccagg aaagcaggga tggcacagag cccggttgcc
    18781 agcaccacga atatgatcgc ggtcaggatc ttcctcatgg ctgacctccc tcgtatctcg
    18841 tagttgcccg tatggcgatg atgaagatgg cagtggtgag cccggccccg atggcagcct
    18901 gcgagattgc cacgtccggg gcctgcagga tatagaactc gagcgagatg aggaaactga
    18961 agaaacctgc agcgagtgct gccgcaagca ggtcgcggaa atgaaagacc atcagcgcgg
    19021 aggcaacaag cccggcaatt accagggcat ggatgaccag gtcgatcatg gtgtaaactc
    19081 ctccagccgg tccacgattg ctggagctgg ctttatcccg gccttgtgtg cgccgcgtgc
    19141 gatggcatgg gaccctaccg cgttcgtgaa tgcaagggag atcgcagcga tcagggtatg
    19201 aacagagagc gtcacatact gcccgtcccc gctcgaaagg aacagcgcca caccgtagat
    19261 gatcaccgag agggcggtga acattgaccc gaatgtggtc atcttggtcg tggcgtgcat
    19321 cctcgtatag acgtcgggga accggagcag ccctgcgatt ccgagcgcat tgaagaatat
    19381 cccgaccgcg aggcatgcca ggataaggac atccgcgatc acagctcgcc tcccaggtac
    19441 ttggcaatat agagcgtgct cacgaaggag agcaatgcat agacaatcgc cacatcgatg
    19501 aagatgatct gctggtaggc cacgccgagg agtatcagaa cagcgacaga gagcgtgttg
    19561 acggtgtcta ttgccactgc acggtcagca gcggtcggtc ccaggaacaa ccggatcagg
    19621 gaaaggcaga ccaggatgac gaggatgcac actgcgatat accaggggtc gatcattccg
    19681 caatcctccg tatccaggcg gggaggttcg caagcgagaa aagctctctc tcctcaacct
    19741 gcggcctttc ctcccacccc tgtgggatat tgatattatg gacgtagagg tcgtggctct
    19801 tctcgtcgat gtcgacagtg atggtgcccg gggtgagtgt gatggaattt gcaagcatga
    19861 agatgccgag gtcggtcctt aagcccgact gcaccctgat tatcccgggc cggatattcc
    19921 cggtgatcac acggtaggcc acgtcgaggt ttgcctttgt cacttcgagg aggaatggcc
    19981 cgagaaggta tagtgggatg agaagaagcc gcagcgggtt tgccatccgg taactgtccg
    20041 acctgcagag taaattcctt gcgactgcgg cggcaagaag cgagagcgca acacctgcta
    20101 ccagttcgga aggagaccag agcaggacgc tcccgcttcc ggcagcaagc aggagataca
    20161 gcagtagggc gagaatgaac gtgagcagga aaggtatcat ccggacctcc cgcacctgtc
    20221 gccccggtat tcattacgtg caccaatcat catggagatc acaggacgcc tgaatcagga
    20281 tataactatc gggccgaata gacccgaaga ccacacatca ttatgtataa tctatcatga
    20341 tcgattatgt aaatcaaggg acataaacaa tactgtttgt ggcaatgcct aaaacgattt
    20401 ttacgtgatt tattggaatt aaaacgagat ttacacgccc tgtgcggtgg tccatcgaac
    20461 ttttggtcag gcagaagagg aaggcagcta cctgtggata tttatggaaa tatctgcaaa
    20521 aagtaccccc aatgcgctcg ccatcacttc gccggaggat gctctggtgg tgcgattcct
    20581 gccacacccc tgtcctggcc aatgtctgct cccgctgcag cacccgcgcc cgcgaggtca
    20641 tgatcacccc gcctgccgat gcgaggcctg cgtttccttc cgatatttcc cacgtgaaca
    20701 aggtatactc ggagcatttc ggtgttccac tcattccccc cgggcacatt gtgctgctga
    20761 acaaggtgcc tgacccggat cgaatggaag agatcatcgt gggagggggc gtggtcgggt
    20821 cgatcaagta tctccctggg gagtcaaggt gggaagccat cccccgtcca caggcatccg
    20881 tcttcctgac accgcgtcgg aagttcgtgg tcgtcgacaa cggcgcggtc ccgtccatac
    20941 agcaggaggg ggcaagcgtc cttgcaccag gactcgtcga gatcgaacca tccgtctccc
    21001 gcggagacga agtctttgtc cttgacgagg agagagccgt tgtagccgtc gggcgtgcaa
    21061 aggtggatgc aaaggaggcc gggaacatgg agaggggtgc aattgtccgg acaaggcgga
    21121 acttcccttc gaactgcaca aggggggagg cgacgtggga ggatgcagtc gaggcatctg
    21181 ccggggtact ggaagaacag gaggcagaag cgatctcgtt tatccgggat gtcgccgcga
    21241 ggaatcctct cccggtgaat gtctcgtact cgggtggaaa ggacagcctc gcaaccctcc
    21301 ttctcgtgaa taaagcgctt ggcaaagtac ccatgctctt tgcagacacg gggcttgaat
    21361 tcccggaaac ctgccgcaat gttgaggaag tctcccgcga atttgggaac ccggtcgtgc
    21421 gggcacgcgg cgaagaagcc ttctggaagg cgttccaagt gcacgggcct cccgcggtcg
    21481 acttcaggtg gtgctgccgc accgcaaagc tgaacccggt tgagaaggtg atcagggagc
    21541 actggggtga atgcctctca ttcatcgggc agaggaaata cgagtcgttc aggcggatgg
    21601 caagcgggag ggtttggaga aacccgcacc tcaaaagcca gctctctgca gctcccatcc
    21661 atcactggac ggcactgcag gtctggctgt acctgttcag ggagaacgcg ccctataacc
    21721 cgctttacga gtccgggctt gaccgggtcg ggtgcttcat gtgcccatcc agcgatgtgg
    21781 ccgtactccg gcagatcgaa gagagccacc ccctgctctg gcaacggtgg agctcccgcc
    21841 ttgaagagtg gcagaaagaa aaaggtctcc ccgactcgtg gagaggagag gcccggtgga
    21901 ggatgaaggg ggtgcccgtt gaagaagata ataattgttg attacggcct tggcaacctg
    21961 cggtctgtga ggagaggaat cgagaagtcc gggggtgtgc cggtcatctc gactgatatc
    22021 catgagatct cgtctgcaga ggggatcgtg ctgccgggtg tgggtgcgtt ccgggacggg
    22081 atgcagcaac ttggggatct tcaggaagtc ctcgtggata tctcccactc tgttcccgtg
    22141 ctcggtatct gccttggcat gcagatgctg atggaatgga gcgaggaaca tggggtccac
    22201 cggggcctgg ggctggttcc tggcgttgtg cggaggttcc ccaggagaga aggctataag
    22261 atcccgcaca tgggctggaa cacgctctct ctcactgccc ccgaaaaccc gctctttgag
    22321 gggatacagt ccggagaatt catgtatttt gtccattcct attatgcaga tacgcaggcc
    22381 gtgtacacca tggcagcgac tgagtatgtc tgccccttct cgtcagttat ctggaaccgg
    22441 gaccgggtct ttggggtcca gttccacccc gagaagagcg gtgcagcggg cctgcaactg
    22501 ctccggaatt tcatcgcaat ggtctaaaaa aaaattcgat cgtgatggtc tgaccagatt
    22561 aaaaaaagaa cagacgggga ttttttactt tattgcttca gggatcgcgg agttgccatg
    22621 ccgaaagtga gaaggggact tcccttgttg tccgtgaggg taagcagtcc gttttcggtc
    22681 tggtagcccg acacccgctg caggaatgcc atgtatatcg cctcctggct catgacacct
    22741 gacggtgaaa tgcaatactt ctccgtggcc gcaatatccc ggatggatac ttccccctct
    22801 tttgccgagt acgtgccccg gtagtggttg caaccagaag agccgctgat gtcacctttt
    22861 ccgtcgaaaa atgcagtgat ctccgtgccc tgaacgggtt cggccgatcg tcctgacgcc
    22921 gtgtggaata cgaccaggta ccacatcact ccctcgagcc cggcctctcc tcctgggatc
    22981 gatacaacgg gcggcgctgt ccccgcgggc gggctcctgc cgggagatcc aggggtactg
    23041 cacccacagg tgcagagcat aaggacaagg atccctgaaa ggaggcagcc aaaggtctta
    23101 ttcatgcatg aatactcggg ggctgctatc ttaatctatc ggtcacgaaa gttgtggatc
    23161 atgtactgaa agccattttt ttactttcaa aatggctgtg ccattttcat gaatgcgtat
    23221 gcgtgtatct cgaagatcat catgtagttt tttttacctt gaggaaaaaa ccgggcagga
    23281 atccttccag atacccgttc actctccgta tggtaattcc gccttattcc tacctccagg
    23341 gacggcatca tcccccgggg attattccag aatattgatg cgtggatctc acccagtcgt
    23401 aatgtgaaga gcgtgaccag gcttgatgat tttctgggtc cattgcatca ggggatctgg
    23461 gagattaccg tgggaaagga tctggtcacc gagcctctcg attccggatg ggagcggtca
    23521 ctcatcaatg ttcccacccc ggggactatt gcaagctttc ggaagggcca gtaccatgtg
    23581 cacgagactt ccactgaatg gaaagtgcac ctggacaggt atgacccgaa agtccatcca
    23641 ttcctgcacc tcgtggacga tgctccgctc ctcctgatgg ttgccgatac ctttgtgacc
    23701 ctggttgcca ctgcaagggg cactcacggc aattacatgg acgcagagtt aaaggagcag
    23761 aagcgtacct ggcaggtgct tttccttttg ggatttgcac tggcacttgt cggaatatgg
    23821 atctttgccg atccgctcac cactttctca tggataattc acgtccttct ccctgccggg
    23881 attatcgtgc tcgggctatt cgtcgcccgg aaggggataa gcttcatgcg acgggccatt
    23941 gtatcgcccg ggagcttctt tctcgggata tccgttgtgg tgctcgggat agtgaccttt
    24001 gacctccctc tggatgcgtt tgtacagctg ctgctgctcg ttttatcgtt ctgggcgttt
    24061 ggaagcgcct acatgtcgtt ctcgcgggtg gccaggggaa gaaaagcagt gcctgaagga
    24121 tttttccgcc gcctcgggac aggagtgctc tccttcctcc tggcgttggg catactgctt
    24181 gtacctgatg cgatgctcga actgctcatg gatatctttg ggatactggt cttcatgctg
    24241 gggatcgtca tcagtacgag cgggtggagg ttgcgggaga agatgcggtc ctcccgggca
    24301 aacccggcca atgaagcggg gcgatgaacg gcgggagtgc ggagccggtc attgcgtggc
    24361 ctcgatctgt gcatcactct gccgcatgct gatgatctgc cctcgaatag tccctgtcct
    24421 tgcgcgttcc catcaaaaag gataacaccg ctcccggaga tttgtgagta tgcacgccag
    24481 ggtcaggtcc gggcttcgta tcatgaagga gatctcaaag gcgctgatct tctttttcct
    24541 ctttgtgatc gcggtgccgt tgataattgg cgtgatacag gaagtaccgg caggaaacat
    24601 cctgagtttt cttgcatcga cgttccttct ccaggctgcg gcgccgccgc tgggggggcc
    24661 gctcggcctc tctcagatgg tcgtgcttgc cgtcatggca tcgtttgcga tcggcatggt
    24721 ccttgcaatt cttgaaatct gcgaaagcct tgcgctcact tcggaacgtg tctccgggtg
    24781 gattgagaaa gtgggcaaga agatggagag ataccctgcg atccagaagt acggggcaat
    24841 ttcatgcacc ctgatcgcat ggataccagg gatcgggctc tatggcacac cgatcattgc
    24901 atggatactt gggtggaacc ggtggcttgt cgtcgttttt accactgcag gttttgtgat
    24961 cgctgcagcg ttcgtgctct ttgtggccca gcacatccac agcatcgagg atgtattcat
    25021 actcggtgtt gcaggagcag ccggggtcat cgccctgatc cttgccggga agtatgccag
    25081 gaagagagcg tcatagtccc tcgccctcgc ccggcggaaa aaaaatgatg tcaggactca
    25141 gaaaacctgg cacaggcaca gtgactgaga tatccggcga ggaccatcag tatgcccatg
    25201 aggaggaagt agatggcgag caggtacccg aagagtatga tgctgcctat gaatggatag
    25261 aagatgatga gcagggcgaa tatcagggag aggattcctg aaataaagta aagggccctt
    25321 cttttccctt cttcaaagac aaggccgaag agcacctgca tcataccgaa aatgaagatc
    25381 gcggcaccga taatctcggt catgatagcg cccgcgatga tgggatagac gatgattccc
    25441 actccgataa gcagccctat caacccgcca aagagcagga gtgtcgagcg tgtcgcccct
    25501 gaaggcaaaa aaagggacga gaggagcatg atgactccga ggatgatcac aagcactcct
    25561 accagtgtca cgacggcaat cgcggtgagg tccggaaaga gtatcatcag gactccaaag
    25621 agtatcgaga ggagcccgag gatgaccagg gcactccatc ccctgtcaca tggagtgaca
    25681 aggattacag tatcagccat agtgaactat ggcacaggcc atggctatga atgtatcggg
    25741 gatcccttca gggaggaaaa aaccgtggac caaaattatt tggaggcgac aataccattg
    25801 cgggaataaa aaatccctct gaaaaaagat atgcgcgggt tgctctcaac tcccggcacg
    25861 cgtcacctgg aactcttatc ccccatggtg tgcagcgtga tcatcccgtt gcacgagttg
    25921 cagacgtaaa attcaggtgt ccagcaggaa gagcatgccc atctcccgca caggatgcac
    25981 tgcctgagtt catgataggc gaacgggctg ttgcagagat cgcagaggaa ccccgagaag
    26041 tgtgccgggt ccttgtcggg gggctccccc tggcctctca tcattgggac gtcttttttc
    26101 agccgtgaga ccaggtcttc cttctcgctc tcgcgccgtt tgttacctga tgacctgctt
    26161 ttccagagtt tcaagccgct cctccaggtt ggccatctgc tccccgaact gtctctcaaa
    26221 ttctgtgagc gaagccatcc gtttctcgtc ccgggagagg tccgcaagct tctcgtcccg
    26281 gaactcctgt gcctggggct ttcgcagcag agaccattcg tcgatcatcg aatcgaagcg
    26341 cctgtcgaga tactcatcga gcctgctctc catcggccgg gtgatctcga gttttccgga
    26401 tgaccccctg aagtagtagt agatgatgaa gccgatgagg ataatcagca ggaatataag
    26461 gagagcttcg gtcactgtca tctctttgcc cccttcaggt gttccaggcg tttctcaagc
    26521 tctgtcatct tggcagtggc ggtggcttcg aacccggcgc acatcctcgc ctcgtcttcg
    26581 attgcatgga gcctcgagta gaggtctccg aggtcgttcc tcgtggcaag gttccaatcc
    26641 tcgatgagat ccttcatgcg gcggtcgatg taccgctcca tgtagtcaag tcccatcata
    26701 atccctccag cttcttcagc tccttttcaa gttttgccgc cctggcctcg agcgaggtcc
    26761 ggcccttctc caggaaatct atctgctggt gcacgcggga gagcctccgt tcgagttctt
    26821 ttaggtgcac gctgccaaga aggccccact cctcgatgat cgccggcagg ttctggtcga
    26881 agtatgcatc gaggtccttg tccatggtcg ggagccgctg gtcaatgtag ccagggatgt
    26941 ccttgatcga atcaatgaat gaatgttttt caccaattgc tccgggtgtt tcatagaccg
    27001 tcatttgtga tcaccccagt ctgtcctctt cctttttaat cttctcaatc atgtggtcga
    27061 gcttggtcag cttgaccttg ctctcgagtc tctggatccg cttctctacc gcgctctgct
    27121 gggcgaagat atcggtctcg agcatcgtgt tctcctcgtc aaggctccga agttcctcct
    27181 gttttgcggg agagacgcga aagaaaggat gccctttctg ctcgaggtct gccttgatca
    27241 gctggagttt ctccttctca aggtccatct tcgcaatcct cgtctttgta tacatgatcc
    27301 ggacttcccg gatgatgaaa tagacgatga tgaggccaag gatgatgaga aggaggggga
    27361 aggactggtt cacggtcacc catatatctg ccatcggttg tctcccctgc ttacaggtct
    27421 ttgtcctgta gtttcttctt cacagtctcg gtgctcttct cgattgattc ttttgcaacg
    27481 ctcgctccct cttttacctt ttcagcagcc tgtgaggctc cttccttgac tttctctgct
    27541 gcctgggttg cgctctcctt caccttcccg gcagcctcag gggctcctcc cctgatcttc
    27601 tctgcagcct gtgctgcacc ccccttcacc ttttccgccg tcgtttctgc ccctgttttt
    27661 acccggtcag ccaggggcgg tctcctcgtc acaatcacgc tcgaccctgg ggctgcaccg
    27721 atggcggcaa tatcctcggg gctgagacgg atatgccctt cctcaaccag ggtgtcggca
    27781 aagaccgcaa caaccacagg ttttgcctct tccccgaggg agatctccac atcgtcccct
    27841 tctttgatcc caagttgtga aaggtatgat ttgtgcaacc tggcacgtcc cctgctcgga
    27901 aaagcacgtt cctgaacctt cagtttcaca gaatccatat gagagaaaca tgtgctgaca
    27961 atttatatat tttattctga acgaactgtg aaattgatga aaagaagtaa ccaaataaaa
    28021 aatgagtggc aattttcggt tttttgaatt tcttcctgca ctgccttcca cagtgcggct
    28081 catcggggca tcgcggccag tgcgtcggcg atgtcgcgtt cagcagaaac taattatctc
    28141 cagcccactg agagcatgac atgagatcag cggttttttt cctgctcggc ctgctcgtac
    28201 ttgcggggag cgtgaatgcg tacgtgctca cgatatcggc cccatcagag atcagggccg
    28261 gggcacccct cgaggtcgtg ggaaacacca cttttccggt gggaacccag tttgacctgg
    28321 tgatctatcg cctgcaaccg acgaccccgg cggaggtcac gagaacgatc atcatcgtcg
    28381 acgagtcaaa atcattccag gcctcgtttc ccacgactgg catggaggcg ggagactaca
    28441 aggtcgaggc caggttcctg acggatcccg ggtcgaaact cgggtcggac tcggtcacaa
    28501 tgagggtatt ccggattact gacaggtccg gggagatcgt gctgactgtc cccaccgacc
    28561 aggtgctggg agaagcgctc cgggtggaag ggtatatccc gaaggcaggc gtgaccacca
    28621 ttaccctgaa agtcagcggc ccgcagggtg ccgtgctccc cccacaggat atccgcacca
    28681 ctacccggac agggagggac gaggggtatt tcatgaaaat gttccccgta tcagagaggg
    28741 gcaattatta cgtggatttc tatgatgtca aggggttcat gacgaccgtg aagttcatgg
    28801 tcaatgcccc accgcccggg acagaggagg ttgtcactcc cggggcgacc gcgtctccca
    28861 cggagagcgc gattcccacc aggacgccat tccccgttgc cgggtgctgt gccgcgctta
    28921 tcggagcagg gctgattgca cggagggtcc ggaggatata acagggcaat ctcatttttt
    28981 catcctggtg ccagtgatgc ccgtgacgcg gtccccctga cgacgattca ccagaactcc
    29041 tcatgctgac ctgtgggctg cctcgcttcc tgcctacggg catgaatgtc caacggtgat
    29101 tgccccccca aaaaataatt atcaaaagat gggtcgggtg gtatgttggt gaggatgggc
    29161 agagatatct ccttcatcac cgctggagat atttcggatt gtttccctca tttcagcctc
    29221 ctccacaccg ccacttcgta aatgatgagg aggatgatca ccaggcatcc gccaaggaag
    29281 aatgcaagga ctgggtcgag ggccagtccc tggacggggg caaccgcaga gggagcgggc
    29341 tcggccatga tcccgaccga cctctctccc tggaactttg caacgtcttc tccgcccatc
    29401 cctggtggag cagaaaggat aggcagcatc gcgtaagccg ccacagaggc aaggataata
    29461 atgccgaaga gggttgcgta cttgagcagg agagacctga tgttctggac cctcggcgcc
    29521 acgataagga gctggtcctt taacccatag acttttatct ccctcccctt cacgctgtaa
    29581 cgtgtctcgc gtatctcgag gaggcctgca tcgaggaggt tctcgatgtg atacttcacg
    29641 gttcccattg gcatggcgag cgtcgacgca aggtcgcccg cagtctttgg cctctcctgg
    29701 agtgcatgca ggatatcgtt cgcagcctgg cttgcaattg ccttccctat cttctgggcc
    29761 cgctcgtctc ctggctggag cacgaccacc tctcccgccg gagcgccggc ccccctatcc
    29821 cctgcatcat cgtgaagagt gtcggtcaac aacgcatctc cccttcgcct ttatcccctg
    29881 agcgcctcga gaatccgttc cctgcccact ttgagggcaa ggtcgacctg gtgcacttca
    29941 ggacctccac cctgtgccat tgtccttgaa cctccaccct tgccgccaag gagcgagcag
    30001 acctggccga tgatctcccc tgcattcacc ttctccgtgc ccgacatcaa aagcacggtg
    30061 gccggctccg acctgtttgc caggagcgcc actcctccct ttccggagat ctctcccccg
    30121 atcgcagcca gttccttgcc ggagaggtcg atctgcctca caagaagcgg tgtgctgttc
    30181 actgtctcga gggtgaggtt tttgatctcg agttcgacga ggttctggcg gagacgttcg
    30241 atctccttct tctggccctt ccactcagag aagaaccggc tgacggttgc cggcaggttc
    30301 tcgttctgga cagaaaggac gtctgcagcc tgggctacca gtgactcaat gcgatggacg
    30361 tattccacgg cggccaggcc tgcagcaaac tccaggcgtt cgatcccgtc ctggatatgc
    30421 tccacccgga tcaccttgac aaagccgata tcgccggtgc tgcggcagtg agtgccagca
    30481 cacgcttcga catcccctgc gacctgcacg atgcgaatct cccgcccggg tggaacgcca
    30541 ccctggtaaa gggcaaaccc gtagagctgc tcggcacggg tgcggtcaag gtgcttgacg
    30601 tcgaccacct ggtcggccat gaccatgcgg ttggcggcga tctcgatctt cttcaactca
    30661 tcaggagtga tgtgcttgta gtgccggata tcgagcctcg agctctccag gcccttctgc
    30721 gctcctgcct ggtggatgtg cgcaccgagg acctcctttg aagcatggag gagcaagtgg
    30781 gtcgcggtgt ggtggcgcat cagggaccag cggcgctctt cgtccacgat acctttcacg
    30841 cggtcgcccc gccgcagggt accgcctgtg acgtgatgga cgatcacatc gcccactttt
    30901 ttcacgtcgt ccacccgcac catgttctct gcggtcatca gcatgccgca gtccgggggt
    30961 tgtccccccc cttcggggta gaagagcgtc tggtccatga ccacgtaccc gtcaaagaag
    31021 tctatgacca tggcatcgaa ttccatgtcg cagggctgct cgtagaagag cttccttgtt
    31081 gcagggaggg cagagatacg atcgcggtaa cgggcgaacg gatcctcctc tgcaacaggc
    31141 cctgattcgg agtgcatgtc cgcaatcagc gagtaaaagt tgtccggaag gtcaaccacg
    31201 gcaccctcgg ccagcgcgac ttccctcact atctcgggcg ggattccgtg cgaatcgtag
    31261 agggtgatca cctcttttaa gggaactctc tcccgcttgt tcttgtaact gcgggcgatc
    31321 cgctgcacga tcttcgtgcc ccgctccatc gtggcattgc tcttctcaac ttcacgctcc
    31381 actatctcac gcacgatgag cgtgtcctgc tcaaacgagg aggttccgac gattcgcatc
    31441 tgctgctcga tgaggtccgc caggggctct tttatcccaa ggtcattcat catcctgagc
    31501 gtcctccgga gcaccagccg caccaggtac ccttctctca cgttcgaagg cacgatgcag
    31561 tcgccgagca tgtaggcgag gcagcgggtg tggtcgacga ttgcatacac cttctcgatt
    31621 ggggtgatca tcttgtccag ccgctcgatc gggacgtcaa tcgcggtggc gaccttcttc
    31681 cggagctggt aaaggttggt ccccgagata tccatgactc ctgcgaattt tgcgttcagg
    31741 gagaggatct tggtgtactc cttgtcctcg agcgcatggg cgaggccggc ggcctccatc
    31801 accctgctga ccatctcggg gaagacggca tcatatatgg tgggtgagcc gcgggacgcc
    31861 catacgaacc gttccaggcc gtatcccgta tccacgatcc agttggacat gggataatac
    31921 atctcccctg caaggtcgat cccgggcttg ccggtcttct gccgccccag gttcatgaag
    31981 acaagcgtcg ccacctcgag gccgccgatc agcacttcca ggcttgggcc ggcgttgccg
    32041 ccaccgatcc aggggtgctc tttataggtc acgcgagaga ggtccccgcc gatggacgag
    32101 atgaactgct cgcagagttc cagggttcgt tccttccagt atatctcctc aacatcggag
    32161 ttgaatgcgt ggtgggccat catctcgaag agcgtgaggt gccggcctga ccggcctacg
    32221 gaatcaaggt cgttcaacct gatgcagggt tgcgatatgg tcagcgggtt tgcgggaggg
    32281 gggacgacgc ctcctgtcac ccagggctgg aaatcggcta tcgatgcgat ggtgagataa
    32341 atatcatccc tccaccgtgc cgcgacaggg tagcggttga ttctggtgtg gccgttcttt
    32401 tcgaagaatg agaggtatgc ctcccgcatc tcgtccacag tatggcgcct gaaaatcggg
    32461 gcaccgataa aattgtaggt ctcacaggga gcatcgccgc agatctcggt atccggattc
    32521 cttgtccaga aggcagatcc gcacttcttg catatcttcc gcgtgagtcc gtgggtctta
    32581 aaatattcga gctggtattc ctcttcgagc ataaaaccct cgtgttctca atttattcgg
    32641 tttttcacga taaaaattgt tggattccgc tgcttcatat accccaggtg tcctggtacc
    32701 actcttcgcg ctctgcataa cccccgcata tacttgcaag gcgggccgca aggaggtgcc
    32761 agcagcattt tccgcggaat acaaagtccc tgcaggtaca gaaatcctct tccaccacgt
    32821 aactctgcga cgtgcctgcg acgaccacga aatcgaggta cttcctgacc atcccgttct
    32881 tcaaggcatg gagggctttc tgtccgcggt ctccgtatat acggacgaat tcgtcggaga
    32941 gccggggggt gaggacgccc tcctttttca gcaggcgcca gaactcttcg ggatccatgt
    33001 atggagaagc gggggatctt caatgtatgc ccagcccatc ttctgagcga gtgctttcat
    33061 ggcgggggct tccagtgcat aatgcgttgc ttccacgaga ggaatgggag agcccctggc
    33121 caccgagtgc cgcaactctg ccgagaggag ggaatctgca ctttcctctg ccgcctctgc
    33181 gataagatcc ggggagaagc ctgaccctcc caccaccgcg agcatacccg ggagtcgcgg
    33241 gctcccccat gcccgcagcg ggcagcagag ccggtcggcg atctcctgga ggcccagcct
    33301 gcactctccc accactccga gtgagagggg ccgtgcatct tttagatcaa ggagacctgc
    33361 caggatgtcg ttgaccccgc ctggggcatg gtcgtaattc gtgtgcagta cgtagatgtt
    33421 cattcccgag gcaacggctc ttcgcaggag tgcggcgatt gagccccgga acgctgtgac
    33481 aggggtccat aggggcgtgt ggtgcacgac aagcatatca gcgccctctg atacggcctt
    33541 gccgacggtt gcaatcgtcg cgtccagcgc acagcagatg tgatccaggt tttccacacc
    33601 ctccacaaca agcccgatct tcccgtcatc ccattcctcc gcaagatcag ggggtgccag
    33661 tgcttccatg atgcgggtga actccccgat atccatggca ggtatatcgg tgttcaggct
    33721 tattaagagt atattactat tggtaatacg aaacatcttt aaatattctt tatcgaatat
    33781 tattgtatga agaagaccat ccatcagata aatgaacgga ttcgtgacgg gaatgcccgg
    33841 gtggtcaccg ccgaggagat gcctgccatt gttgccgaac tcggcgagga aggcgccctt
    33901 gcagaggtgg acgtggtgac caccggcacg ttcggggcca tgtgctcctc cggagggttc
    33961 ttcaacttcg gccacgcgga tcccccgata cggatggagc gggtctggtt aaacgatgtg
    34021 gaggcgtacg cggggctcgc agcagtcgac gcgtatatcg gggcgaccca gcagtccacc
    34081 acaagagaag accagtacgg gggcgcccac gtcatcgagg atttcatcgc aggaaaatcc
    34141 gtggagctgc gggcgatctc ccgcggcacg gactgctatc cccggaggac catcaccacc
    34201 gatctcctcc tcgagaacct caatcaggcg atcatgtgca atccccggaa cggctaccag
    34261 cggtacaatg cagccagcaa ctcgaccgag agggtcctgc acacctacat ggggatgctc
    34321 ctcccctcgt ttggcaacgt gacctactcg ggcgcaggtc tcctcaaccc catctcaaac
    34381 gatcccgggt tccggttcat agggtcggga gtcccctgtt tcctcggcgg ggccgaaggg
    34441 atggttatcg gcgaagggac ccaggcctct cccgccgcgg gttttgggac cctgatggtc
    34501 accggcgaca tgaaaaagat gaacacggag tacatccggg ccgcaacgat gcatggatac
    34561 ggagtgacca tgtacgtggg gctcggcatt cctattcccg tgctcgatgt cgagacggtg
    34621 aggagcactg cggttaagga cgaggacata tgggtggata tcattgatta tggggtccct
    34681 gcgagggacc gcccgactat cgggaaggtg aactacgcca tgctgaaagc cgggcacctt
    34741 gagatcaacg gcgaggaggt gcggacatcc tcgctctcga gcctccgcag ggcgcgaagg
    34801 gttgcagaag agctgaaagg gtggatcgag tccggaaaga tggcagtctg cctcccgacc
    34861 cggcggatcg atccgggccg aagagccgcc ccgatgcggg agactgtcca ggggccgagg
    34921 gtgcttgaga tcatggacag gcgggtgatc accatatccg agaatgaacc catcagggac
    34981 gctgcacgga aactcctgaa aggggagacc aaccacctcc cggtgatcaa cggggagggg
    35041 gtgctggtgg ggatcgtcac cacttacgat gtctcgaaag cagtggtgaa ccccgcgaag
    35101 atctctcatg tgaaggatat catgcgccgg aaagtggtca ccaccacggc agacgagccg
    35161 gtggacatcg cggtcaggaa gctcgagaag cataatatca gcgcactccc ggtggtcgac
    35221 agagagtacc gggtggtggg gatgctcact gcaatggacc ttggcaagct cttcggcggg
    35281 aggtggctga aatgaagctc ctggtcacct tctcgagaaa ggaaggaaag aaacccatca
    35341 ttgcccaggt ggtgcgcgat acaggggtgc tggtgaacgt ggaacgggcc ctcatcgatt
    35401 catcggaggg agaggcgctc atcgatatcc ctgatgagtc ctgcaggatt gtaaaagaca
    35461 agctggcaga tctcggcgcc tcggtgcgca tcctcgagga ggagataagt ctcgacgaga
    35521 ccgagtgcgt ggactgcggc gcctgcatca gcatctgtcc ccaggaggtc ttctcatttg
    35581 acgaggagtt ccggctccgg atggatgcga aacgctgcgt cctctgcggc aggtgcattg
    35641 agatatgccc ccacggggct ctctcgcgcc gggtagatat ctgatgaggg tcggccggag
    35701 acatcggctt tctcttgata actctcaacc attttcatgt ggtatgcgcg ttttttgcgg
    35761 gcgaacccca gtttttttcc ggttggcacc tttggcctgt aaacctctcc tcatcgattc
    35821 agtttccggc gggcatcgtt gagcgtcggg aactgtgcgt ctcccggaga ggtctcgata
    35881 atatccacga gttcatcaag cgagaggggg acatacccca ccatctccgc gctgacgttc
    35941 accctcctgc ccggcatgtt caggaacggg tattctcccg gttggttgtt gtgcaggtgt
    36001 ccgtgaacca cgaacccagg ataatccggt ggtgcctccg cgggatcgtg caccatcagg
    36061 aagcgccggc tcctccaggt gacctccatg ctcccgctgg cgtgtccaag ccccgaatcg
    36121 tggtttccag ccacgatatg gacatcccct gccaggaggg acaggtagcg tgcagcaggt
    36181 cctgcgttcc gcccatatgc gaggtcgccc aggaagtaga ccgtgtcggt gggcctcacg
    36241 cggaagttcc agttctggat gagcaccgag tccatggttg cggcgtctgg gaagggccgg
    36301 gaggtgtaag tgatgatatt ggcgtgaccc aggtgcaggt ctgagatgac aaacgcagtg
    36361 tcttcctcgc tgaaagaagg gtggtcaatc tggtacccgc tcttttgccg gaacgattcg
    36421 agggtctttt cccacctgtc ctcactgaac gcggccgggc ggccgatcca cgtgcccgtg
    36481 ggaaaatcaa atgcatccat gagcgtcccc ttcctgatga cctcgaggcg aaacaagtcc
    36541 agggtcgtcc ccgccatgca gcgcatgcct tcggcatttc ttctcccggg tggcagagtg
    36601 tccaggaatt cgacgatctc ttctgccttc ctgcggcgca acccaaaccc tgtgctcacg
    36661 tgaaatatcc gctgtcctgc gatctggtcg atccaggtgc aggtccgggt gtggggaagg
    36721 agagagtccc ggacagcggc ggcgagaagg gccagggggt cgcccggggc cgccctgatg
    36781 accgcagcat atccctttcg tccccggagt accagtgcac ccccgagcat ggcggaaaag
    36841 gaggagaagc cgggtgaacg cagaagtgga tcttcgagga gcacccggat gtcgcagcct
    36901 ttgtccaggg taaatggccc aaagagcgtg atgtgggggg catggcgttc tggggggaga
    36961 agtccgaata ttgttgccag gcgcgccatg cactccctgg tccactggct gccgacctgc
    37021 acggtcacca ggtatacggg aggggagtag tgaccggtcc tccgccttcg gtggccggcc
    37081 ggtctctggt gcggcattct atttccagaa gatcgcttga tatatgcgta taaatagatg
    37141 ctgacagcgt atcgggtgat ccgagccctt ttttctctcc caggagaatt actctcatct
    37201 atgatcaggc ggcggttcca tttccgcgag acgatcgcca ccatccttgc agaagaggag
    37261 agccacgtcc aggcagcccg cgaggggttg atgcaggcaa ggcaggagct cgagcattat
    37321 atcgcattcg atcctttctt caggaccacg atggagccct atgaagcggt atcagacagc
    37381 actgtcgtga aaagaatggc cgccgcggga gcttctgccg gagtcggccc aatggccgcg
    37441 gtggcaggcg caatcgcgtg ggcaggcgtg gagtcgatgg cgaggaccgg ggctgaattc
    37501 gccgtgatcg acaatggcgg ggacatcgcc ctcctctctg atcgcgtcct tcttgtgggg
    37561 gtccacgccg gccggtcacc ccacagcgac cgcttcgcgt tccaggtaac tccccgcgca
    37621 gaggttctcg ggatctgtac ttcttctgcc acagtcgggc attcactctc cctcggaata
    37681 gccgatgcag tcaccgtctt ctcccgagat cccggccttg cggatgcctg ggccaccgcg
    37741 atatgcaatg caattagccc ggatgacacc agcatcatcg acaaggtgcc atggggggag
    37801 gttgagggag tcttcgtggt ggcaggggaa tgggcctcat cgtttggcag ccttccgcac
    37861 atggtcaggg caagagtgga ccacgacctt gtcacaggtg gtccatggtg aggatcaaaa
    37921 agactacatg gttcgctaag aagatttacg cgtgcaggtg cgcgaaagcg tggaggtgtg
    37981 aatccttggg aagaccccac cgtgcacaca atttccggcg ggtatgatgg gggtgaatgg
    38041 aaggcgcaat ggacaggacc attctcgtcg ttgacgataa ccgcaggctc gttgatctcc
    38101 tccggaccat cctcgagatg gaggggtaca tcgtccatgc agcctatagt ggcgacgagt
    38161 gcctcgcata cctcgacaga gaaaggccag gcctcattct ccttgacatc gtcatgtctc
    38221 ccatggacgg ctggacggtt ctcgagcgca tacgggagag acctggcacg ggaaagaccc
    38281 gggtgatcat gctgaccgcc aggcatccca cgagaaagga agcagagaag tatgccgcgc
    38341 ttattgatgg gtacatactc aagcccttcg atgtgcgcaa cctcctggag agtatccgtc
    38401 gtgcctttgc agaacaggaa cgccttgaag gcgtcatcag gagcgcgcac gagaagggca
    38461 ccaagcgaag tttccttgcg gaatactgca ggctctcgcg tattgtgaat gcgcaggagg
    38521 cattgagagc cctcctctcg ccgggacccg gccagggaga gggggaagag gagagcgatg
    38581 aagccaggaa acttgcgacg atgaagcaaa tcatccagca ggtggtcggg ataccgggtt
    38641 tggatgaata acgatctgcc tgccaaggta acaggccatg gatctgtttt cagtggggcc
    38701 gcgaaaaggc ccgccatcca tgcacgatcc tagagcgagg catcaataaa cgcaagcgcc
    38761 ctccggtatg catccctgcc gcggtaattg accaggacct ggtcgccgtc actgacgctc
    38821 atcaccggtt ggtcgtcctc gacccggaag atgacctcga gaagttcgag ttccgcgatc
    38881 atcacgccct gcaccagcgc agatgcgggc tggaggatca gccgctcctt ctccagctca
    38941 cccggggcct cccggaaata gtagtagtag acctggtatg cgttcagaaa gtcggataca
    39001 agcagctccc ggcggactgc tgggtcgacg gtgtcgagtt ccctggctaa aagcgcctcg
    39061 tcctcgtccg ccttctcgag gaggtgctca gcgattcccg caagttcccc ataagagggg
    39121 accttctccc gatcttgcat atcaagggat ggagcggcgg ctccttatag atagtgaatc
    39181 tcaatctttg taaggttcca tggatgagag ccgaaagatg ggccagcatg tcattcccgg
    39241 atgcgggcac tgtagaggtg agggtttccc acgccctcaa aagcgcgatg gccgcgtatt
    39301 cctccacgag cattatctcc tcttttttcc ataccttacg ctacgcgaca gcacatgaga
    39361 gaagaagagc tggactggca ggtatatcac ctgctgatgg atgatgcggg cagggacgag
    39421 gatgcgcttg ccgcgcttct gcattgcacg ccgggagagg tgcacacatc catcgggcgg
    39481 ctggagaagg ccatgctcct cgaatgcacg ccgggagggg tccgtgtgct ctctgtacag
    39541 gagatggcac tgcggtgcca ggcccgctac gaccggagtt gtccattctc gattaagggc
    39601 ggagtgatcc gcttgaaagg cggaagtgac gaaaaagatg actgaaatat tcgtcctccg
    39661 gattggacac cgtcccgagc gcgaccagag ggtgaccacc catgtcgggc tcactgcgcg
    39721 ggcgctcggg gcccggggga tgtaccttgc cgcgagggat tccggggtgg tcacaagcat
    39781 ccgcgacgtg gtctcccggt ggggtggtga ttttctcgtg caggaccagg tctcctggaa
    39841 gaagtgcatc cgggactgga aggctgccgg gggcattgtc gtgcacctca cgatgtacgg
    39901 cgaggggatc aggagccacg aagaggcgtt gcagaggtgc caccgaatcc tcgtggtagt
    39961 gggggcagag aaggttccgg gggaggttta tggcctggcc gattacaata tctcggtgac
    40021 cgggcagccc cactcggaga tagcgggcct cgcggtcttc ctcgaccgtt tcttcaaggg
    40081 aaccgaactg gagagggagt ttcccggggc aaagatacgg gtggtccctt cacccgaagg
    40141 gaagatcacc gaggagcaat gaagggcgca gtgctcgtct gcggatttgc gacccggcac
    40201 gtggccaggt ccgcccatgc cgccggatac actgtctacg cagtggacca cttctgcgac
    40261 caggacctct cctggtatac ggccgaccga gagcggttcg aaaacctgtc agaccttccc
    40321 ggcgccgttg agaatatctg cagccggcat cgcgtggact ggctggttcc cacttcgggc
    40381 gcagagacgc tccccgggca gatccccgtg ctcgggacgc ctcccgggcg ggcagcccgg
    40441 ttcatggaca agctgctcac ccaggaattc ttcgagtccc ttcaggtccc tgtcccaaaa
    40501 atactcgggc cgggctgcta ccctgccatg gtcaagccga gagcagggtc tggcggctgg
    40561 cgcaacatgg tggtccacag cgatgcggaa cttgcatgct ggcttgagac cttcgagtgc
    40621 ccgccctatc tttcccagga ggttgtaaca ggtacccctg catcagtctg ctgcgtggcg
    40681 gacgggaagg gcaacgcgcg ggcaattgcc gcaaactacc agatcctcag ggaaaaagcc
    40741 ggagcacaat tcgggttctc cggctcggtc actcctctcg ttcacccgca ggcacagcaa
    40801 atggccgaga tggcagagaa gattgcatcg gcctctgggt gtacaggcac tttcggcatc
    40861 gactttgtca taggagatga gccctgggca attgaggtga atccccggtt ccaggctacg
    40921 cttgatactg tcgaggccgc aacagggctg aacctctttt cggcccatgt gcgtgcctgc
    40981 cagggagaga tccctcccgg ccggccttcg cctgtcgggt ttgcggtgcg ccgcatcctc
    41041 tttgcggaca gggacatggt agtgaggaat gacctctctt ttctcgcacc tgcagtggca
    41101 gacattccct ggcccggcag tttcttcgaa gaggggcatg cggtgatcag cgtctatggg
    41161 tggggcagga atggagaagc agccatgaaa ctgctggata cacatatgaa gagggtccga
    41221 caatatatgg gttgattgcc ctatggaaga gatcctcgca aataccccga tacgtgcata
    41281 cctcgcccgg ctgataggag aggagggcat ctctctcctg gagagcttcc cgagggaagg
    41341 cgagcattcc gatgaggaac tggccgcgca gaccgggatc aacctcaact cggtcaggca
    41401 caccctctat accctttacg agaagcggct cgccgagtac cgcaggatca agaataacga
    41461 aacgggatgg ctcacttacc tctggcacct ccgtattgac agggtttatg acgtgatcgc
    41521 cgaggacatg gaagcgatac ttgagaagct ggaggccagg gagaagttcg aggaggagaa
    41581 cgatttctac atctgcaagt cgtgcgggtt catgttcacc ttcagccagg cgatggactg
    41641 ccagttccag tgcccctcat gcgaaacggg actggaacac tttgacaacg agatcctcct
    41701 ccgtgcattg aagaggcgga tcaggagcat gaaagagtcg ctcggtcatg cctgattcgg
    41761 agaggcactg ggaggctctc atgagatcgt ccggctgcag taagagggtg atcgcacact
    41821 gcagatctgt ccgtgacctg gcaatcgagt acctgcacga tgatatcatg gaccgggaag
    41881 tccttgaagc gggcgccctc ctccacgaca ttgggagagg gatcacccat ggcctgcccc
    41941 atgcgcaggc cggagcagat ctctgccgca ggcgtggggt gccggaagaa gttgcgaggg
    42001 tggttgagcg ccacattggt gcaggcctca ccgcggacga gtgcacactt ctcgggctcc
    42061 ttcccatcga ctgcatgccg cgcaccctgg aggagcgggt agtcgcacat gcggacaaca
    42121 gggttgccgg aacccgccgc atctgcctcg atgagaggct gttgcacgca atccatctcc
    42181 agaagaggca gaaacaacgt ctctaccgtc tctggcagga gatggagatg ttcaggcaga
    42241 cccctggaac gtgatctggc gtacctgggg gagacgcctg atcccttcga tcaccgccgg
    42301 gggaaagtcc ccctcgataa tgaccacgag cctcggctcc tccgaaagga ccgggtcggt
    42361 gacatagatc tggcgtatcg agagcccttc ttctgcgata accctgacgg cggcactgac
    42421 aatacctctc tcgcttgcgt cttttgggag gatggtcatc accgaaaggc cgaggtgccc
    42481 tgccacccgg gagaggtcgg gtgctgccct catgtgcatg aagatattcc ggtgcagggg
    42541 aagggagagg atgcgctggg cggtggtgtc caccaccctc ctgtccgtat caagcgcctt
    42601 tgccacctgc gttgcaggta tctcaatccc gttgcacgtg atcctgccgt ccgcattcac
    42661 accaaaacca ttttcaagga ggaacctcag cacccgggtc tgggaggggg agtctccaaa
    42721 ctccccgatg atctccgacc acatagtcaa gatctatggg cttgtaatta atatacatcc
    42781 ccgttcacat ctgcacactg atgatccgtg gagaccgccc attgcgctcc agcaggaggg
    42841 atcaggactc cacgggtccc aggatatgga taaccttatt tcgaagcggc atccatctaa
    42901 tatggcacgg agcgagggtt gccgagccag gtcaaaggcg ccagcttgag gggctggtct
    42961 cgcaggagtt cttgggttca aatcccatcc ctcgcatccg aacttgagta tctcttctac
    43021 tgacagcgct tgctccatcc ttggcagagg tggcatggcg ccagtgttcc gggaatactc
    43081 cccgggaatg gaagaatgcc tgcgcaatta tcgctctttt ccaaagaatg attgacccgt
    43141 tgccctgatg acaggcatta tatctcacgg cactgacggg gttaccatgg gagactttga
    43201 tcctaaaaaa cacattgccg aagaggctgg ctatgatgcc aaggataatt acgtgcgcgg
    43261 gctcaagatg cccgagacaa ggtttaagaa catggtgcat aaccaccctc cggatgaaca
    43321 gaagagcatg agaacgcagg ttgaccaggc cgaagccgcc gggcttcgca actaccaggt
    43381 caagcattcg aaaggcttct acatcattga gaatggaatc gtggtgggga gcggaagggg
    43441 ctaatttccc cattcaagcc cttttttgaa ccgggaaatt ttttcccgtt tgtttacagg
    43501 tatggggggc ctgaaaatgg aaatcttttt tacaattcac cggatattgc agaggtatcc
    43561 ggcctggctt ttctgaatgt catgaatttt ccgggtcaaa aacgtggtct gccgaaaaaa
    43621 aaggttcaat ctgagaggaa tcacttcagc ctttttctcc ggatactgtt ccccaacagg
    43681 gaaccagcga ttcccattgc cgccaagggg agagagtccc cgagtggcgt ggagggagta
    43741 ggagtcggtg tgggggttgt gggaacggtg gtggtgggta tggtagtcac atcggttggc
    43801 acctgcattg gcactgcagt ggtgggcgtg gtctcgacag gattgaagac cacgagttcg
    43861 acattgtcga tataaccctc caagaagggc ccgaatgcat aatctcccct cgtggtaatg
    43921 gcgaggcggt ccatgaagaa gagatcgcgt ccgacggata caaagtagcc ccataccagt
    43981 tcgccgctct ccttctcggt cacctttata tctgcgtgct ggagctcctt ggtatacctc
    44041 gccgtgacat ggtaggtcat gttctccttg aattccacgg tctggcaccc gggcaggttt
    44101 ccgcagtagg aggtgtagta gctcgtggct tcccggagct ggttgttctg gtcgatcacc
    44161 cggagagaca taatcttccc gtattttcca ttttcaaagc ttgagaggac gttcgtcccc
    44221 ctcgtgaagt ccatctcaga actgatcagg ccgaaccgga acccgctctc cttctgggag
    44281 gtgaggatga ctacatcgaa atcaatgatg aaggactgcc cgttgtactt cacgggaatg
    44341 aatgcatagc cgtttgttcc cccctctgtc ttgaagtgat acatctccag ggatggatcc
    44401 cagtaatacc gcgtggggct gttggtgttc catcccgggc tctccgagaa atccgactgg
    44461 tataatactg acttcactgt cgtttctgct gcggtggcgg gaaaagccag aatggcacag
    44521 cagccgagca gcaggagcat gagtattctg aatcctttca ttggcatatc tcctcgtgaa
    44581 acggtcccca catgaactcc tgttcttccg taaccacagg gagttttccg gtcctcggtg
    44641 aaacgttttt ttggggggtg ctggtccggg gggggtcctt gttgcagatg aaatgtgggc
    44701 ttaatctcta taattatttt ggaaatttcg agtatcatga ccctgttttt cccttcgtgt
    44761 ccacatccct ggatggactc accgcctttt aatttcgttc catccgggga aaaaagcgac
    44821 atgatttatt attcctgcca cacatggtgt ataaggggca gaattttccg tgtatccaca
    44881 cgctgcgtgt gaacgggggg actgatggac ggccagattt attacaaccg gggtgtggcc
    44941 tttcataaga aggggatgtt cgatgaggcg atctcggatt attcccgttc tattgagatc
    45001 aacccgcatg atgcggaggc gcatcacaac cgtggagttg ccagggcagg aaagcgggac
    45061 tactacgggg caattgaaga ctacaattct gccataaggc tcaaccctca cttcaccgag
    45121 gcgtattaca accggggtgt ggcctattcc ctcatgggcc gccctgagga ggccattgcg
    45181 gaattttcga ccgcgcttga gaccgatccg gatttcatcg aggcatatta caaccgcggg
    45241 ctcgagtatt cacggcaggg tgaggacgag cttgcgattg cagacttcac gcgggcactt
    45301 gaactcgacc ccgcatacgt tgcggcctac aataaccggg gcgtcctctt cggccggaag
    45361 ggaatgcatg acgaggcgat tggggattat tcaaaggcgt gcgagatcga tccgaatttc
    45421 atcgaggccc tcttcaaccg gggaatcgag ttctcccgcg cagggcgcct tcaggaggcg
    45481 gtagcagact atacacgggc actcgaaatc gatcccggga acccggatat cctctataac
    45541 cgcgggattg cactcgggaa gatgggccag tccgagccgg cgatcgcaga ctacctcgca
    45601 tgccttgaga agaaccccgg ttcagcggag gcatacaaca acctgggcgt cgagtacagc
    45661 aggctgaatc tctacgatac ctcacttgag cattacgcaa aggcaattgc tctcaatcca
    45721 catttcgtgg aggcgtataa taaccggggg gtgctcttca atcgtctcgg gaggtacgac
    45781 gaggcgatcg cggagttctc gcgggcgata gacatctttg accaggcggc gtccgaggtc
    45841 catgatgaaa caaaaaccgt ctcccatcct cccgctccag cggtcccaga gcctgtcaaa
    45901 gagaaacacc cggaggattc ccatgtaatt ggggagcctt cccctggcgt gcgtgacgag
    45961 gcgcccatgg accctgcaat tctcgaactc tcccgggaga ttgaccatgc ccctgatgat
    46021 ccggaactct acaacaggag aggccttgaa tattcgcgcc tcgcgtctta tgagcaggca
    46081 atcgaggact tcacgcgtgc catcgagata gattccggtt atatcgaggc atattacaac
    46141 cgggggaatg agtacgaccg tctcgggcgg atagaagagg cgatcgctga ttatacccgt
    46201 gccttaaaga tcaatcccca gtatgccgag gcctgctaca agcgggggga cgagtaccag
    46261 aaagccgggc tctcggacaa ggcaatttcg gaatatacga aggctctcga gatcaatccc
    46321 aactaccccg acgcacttta caggcggggg cgggagttct cccgtatcgg gctttctgac
    46381 atggcaatca gggacttttc acgggccctc gaggcccggc caggctacgt tgaggtgttg
    46441 atctcccgcg gcgaggagta cggaagaaaa gggctctccg aaaaggccat tgcggattac
    46501 acctcggtca tatcgcataa cccttcgcag aaggaaacat accacaagag gggtatggaa
    46561 tacgaaaaga gcgggaaggt ggacgaggca atcgcagatt tcaccggtgc gctcgagcgt
    46621 gacccccttt gggccgatgc ctatctccag agggcacatg catactggct ggcaggaaaa
    46681 tacgaggagt ctgtccagga ctcaacaaaa gcctgtgaga tcgaccatga gaacgcggag
    46741 gcattcctgc agcgcggaat gagccagtca gcgatggaca actcatccga agccattgca
    46801 gatttcaccc gtgccatcga ggttgatccc aggtgtcacg aggcttattt ccggcgaggc
    46861 caggcataca gcaacagcgg gaggagtgag gaggcggttg gcgattttgc aagtgccatt
    46921 cacctgaatg aaaatgtcag ggaaacctac attgcaagag ctgccgagtt tggccagtcg
    46981 gggaggaacg gggaggctat ctctgatctc acccgtgcga ttgaactgtc ccctgacgat
    47041 gacagcacgt acctcgcccg aggtctcctc tatgaccggc aggggatggt ggacgaggcg
    47101 atcgcggact tttcccgtgc aatcgagatc aatcccggga acgagatggc atacaacaac
    47161 cgcggaaacg cctatgccgc ccggggactc ttcaacgagg ccatctcgga cttctcgcgt
    47221 gcgattgaac gaaaccccag gaatgccacg tcattttaca accggggcat cgagtatggc
    47281 gagaacggca tgcttgacca agccatcgcc gacctaagcg aggcaatcac gctacggccc
    47341 gatcttggcg aggcgtatta caacagggga gtcgcctttc acaagaaagg gatgctcgat
    47401 gaggcgatac gcgattacga gcgtgcactg gagttccagg ccgataacga gaaggcgtac
    47461 aacaaccgtg ggaatgcgta cgccggactt ggcctctatg acgaggcgat cgcggacttt
    47521 tcccgtgcga tcgcgatcaa tcccgggaac cccactgcgt attacaaccg cggaatcgag
    47581 tacgggagga aggggttgat agaccaggcg atggaagact tttcccgtgc gatcgagata
    47641 aagcctgacc ttgcagaggc ctactacaac cgcggggttg cgtaccagag ggtcgagagg
    47701 ctgaaggagg cgattgcaga ttatacgcgg acgatagacc ttgcccccgg aaatgagaag
    47761 gcatggaaca accgcggcaa tgcacatgcg gggcttggaa tgtacaacga ggcgatctca
    47821 gatttcaccc gcgccatcga gatcaatccc gaaaacccca ccgcgtatta caaccgtggg
    47881 atagagtttg ggaagaaggg gctcagtgac gaagcaattg cggatttttc ccgtgcagtg
    47941 gaactgaaac ctgaccttgc ggaggcccgt tacaacatgg gcaacatcta ctacaaccgc
    48001 aaccagtgcg aggaggccat ccgcgagttt gaccgcgccc tctccatcca gcgtgggtac
    48061 gcccgggctt acctgaacaa ggctctcgca tgcgagaagc tctcccgcat tccagaggca
    48121 aggtcagcct atgaagggtt cctccggtat gcacgccagg agtctaagga actggttgac
    48181 ttcgtatcgc agagactccg tgacctgtga ggccttcgcg tactggcctg caccctttct
    48241 gaaaccaaaa aaaagatgaa cccggggtat ccccgggctt cttgaggcca agcatcacga
    48301 taccgtgtag gaccctgcgc cgccacggta ttcaacggag ccgagcttta ccccaaggct
    48361 catctgtccc tggctgttct cggccaggtt cgagatgatg acctgctgtg cagagccggg
    48421 aatgatcctc ccgttgttta tgaagaggac cgtggtcgca tctcccgggg gaatggttat
    48481 ggcaagggtg gactggagtc ctgcaaacga gggcacgaag atgtcagtgg agatgccgct
    48541 cctggcaggg ttgccgttca cgaacatccg cacattgtcg aagcggaaac cggctattcc
    48601 gcgtgcattc acatcgatca ttccgctctg gctctgatcc acgaagagct gcacggtgtc
    48661 gccgggcgag aaggggtatg tggatccggc aatcttgatc cagccgcctt ccccggtgac
    48721 gaggaactgc aggtaccctg tggagagcgt tcccatccgg ctcccggtca ggtagatatc
    48781 caccctctcg ggctctttta cctggactgc ccctgtctgg atgcgggtgt tgtacccgta
    48841 ctcgttcccg acagtgaggg tcacatcata tgtccctgcc ttcgtgtagg tatggagggg
    48901 gttcttctcg ttcgatgtcg ccttgtcccc gaagtcccat gcatacgcgg tgggaagacc
    48961 tgtcgagagg tcggtgaaat ggacgatcag cgggagcgtc ccctctgcgg ggagcgcagt
    49021 gaactgggcg acgggcaggg tgcctgctga aataaagccg gtctcggtct tctggcttgt
    49081 cccgtactgg ttgcgcaccg tcagggagac ggagtacatc cctgggttct tgtaggtgtg
    49141 aaccgggttc ttctggtctg atgtttctcc gtccccgaag ttccagagcc acgaagtggg
    49201 ggttccggcg ctcgtatcag tgaactggac tgcaagcggg agctggccgg cccgcgggtc
    49261 ggcggtgaag gcagcctgcg gtggctgcgg ggctgatggc gtcgttccgg ttgctgcagg
    49321 agacacaggc gaggttaccg ctccggcacc ggtcggcgtt gcggtcgcac ccggcggggt
    49381 ggtggtcacc gcggtcggca cggtcgagac agggatggga gtctcctgta cagcggccgc
    49441 ttcgtcagag aagacgagca tctccccgct gcctcccgaa tagatcactt ggaccagggc
    49501 gggaattccc ggcccgtcat actgaatgcg catggtcttt ccgggtgtcc agggccagtc
    49561 ctgggcgtgg aggaacgcga tggaggattc agggatatca ttcccgttga tcctcacgat
    49621 ggtccgcccc ttctggagag ggtctccccc gtcatggtag aggtagacga tgttccctga
    49681 ccgttcgata ttgg
//
LOCUS       DAOQYR010000005        47822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_841_length_47822_cov_8.971131, whole genome shotgun sequence.
ACCESSION   DAOQYR010000005 DAOQYR010000000
VERSION     DAOQYR010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 47822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47822
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_841_length_47822_cov_8.971131"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..173)
                     /locus_tag="PKM87_01135"
     CDS             complement(3..173)
                     /locus_tag="PKM87_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28700.1"
                     /translation="MVNEMPNGGPGMGMQRRGVPPDACICPKCKKEYPKTRGVPCRSQ
                     TCPDCNIPLMGK"
     gene            complement(309..1484)
                     /locus_tag="PKM87_01140"
     CDS             complement(309..1484)
                     /locus_tag="PKM87_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNQ28701.1"
                     /translation="MNVTDLCADLVRIRSENPPGNTRDVIEYIRNFTDSLGIVTQVIR
                     GPGGKDNLVSRTVNGKLLFCGHVDVVPALDEGWTHDPFAGTIEDGKVWGRGTTDMKGG
                     CASVLWACRNWLDSGRELPADLAFVCDEETSGTHGIQALLASKCLVPCDTIVAEPTPS
                     LSPNVGQKGLLRLCCRFRGEPGHGSLYPAKGVSAVMEAFSLLEFVRLLHKREFSPADP
                     ALSGIIGESSKILGEIFGIEDTKRVLSHVMYNPGKIEGGEKANIVAQQCSLELDLRIP
                     WGCSLPDLLEEIRRHAPRGEISVTNSSDPTITPPDSPLVTRLLREIEKVHSATARPIV
                     QWAASDARYLRNAGFPVVEYGPGEIRTLHAIDEHVTIESLENAARVYEGMLGRYVMI"
     gene            complement(1496..1999)
                     /locus_tag="PKM87_01145"
     CDS             complement(1496..1999)
                     /locus_tag="PKM87_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28702.1"
                     /translation="MSSDAEYVHVLEHLYEKSLILQDETMWHPVLAFYYMDALAHLDY
                     VISLMAYHYKSPRVMMAGEYLRCRVDQEKLGDRPRFPAFINWLKREHPKRFETLPTVW
                     RRVYDEDDEARYISFRLVFDRDSKDPIRPHVYRSLIEEFFNAEFLKTLYSDASLSALF
                     EEFKRKA"
     gene            complement(2087..2965)
                     /gene="moaA"
                     /locus_tag="PKM87_01150"
     CDS             complement(2087..2965)
                     /gene="moaA"
                     /locus_tag="PKM87_01150"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNQ28703.1"
                     /translation="MKLKDPYNRPVSNLRISLTPACNLHCIYCHAEGEKDPKAPLSLE
                     ELAEIMKVAAKFDIGSVKFTGGEPCLRADLADIVRAVPERMECSMTTNGTLLADMAHD
                     LADAGLSRVNVSLDSLDRATYMKITGSDCLDDVLAGIDAAIEARLTPIKLNMVLLKGM
                     NDHEVDEFIRFVRGNRDLILQVIELMEFRDCTNHHELKDLEVWLASNSQEILTRRMHH
                     RKKYCIDGAEVEVVRPLHNTEFCAYCNRLRVTSDGKLKPCLLRTDNHVDIRGKYGEEL
                     EELFREAVRRRAPFFQ"
     gene            complement(2943..3551)
                     /locus_tag="PKM87_01155"
     CDS             complement(2943..3551)
                     /locus_tag="PKM87_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HNQ28704.1"
                     /translation="MGSQWGKDRTYLRAVREGYRSRAAYKLQEIQERYHLIRERDNVL
                     DLGASPGSWLQVARGLTGGRILGIDLSPIAPLEGVTTLVGDLTDRSLRDEAGKILGTV
                     NVIISDAAPKLSGHRSYDQARAIGLGQEVLAFACDVLKPGGNLVLKSFQGEDFQALLK
                     EMRQYFLSVKTFKCRASRRGSSEIYIIARNFVGCRSETEGSL"
     gene            3652..4395
                     /locus_tag="PKM87_01160"
     CDS             3652..4395
                     /locus_tag="PKM87_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase sliding clamp"
                     /protein_id="HNQ28705.1"
                     /translation="MLKATIDADILKESIDAISALVTECRLHTDENGLSTRAVDTANV
                     AMVSLTLKKEAFSAFESTKGEIGLDIAKMKNIFGMMGKNDVISLELPEDGHKLKVSFE
                     GYRYSITLLDPNTIRKDPNPPNIDLPGKVVLSGNDLNNSIKAAAVVSDKIALGIDPAQ
                     QLFFMAAEGDTDHIRREFGKNEVNFLNGMEARSLFSLDYLKDMGKVMSRAEEVEVSLG
                     IDHPAKFAFYIAGGHGYVEYLLAPRIEAD"
     gene            4395..5423
                     /gene="priL"
                     /locus_tag="PKM87_01165"
     CDS             4395..5423
                     /gene="priL"
                     /locus_tag="PKM87_01165"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase regulatory subunit PriL"
                     /protein_id="HNQ28706.1"
                     /translation="MQVSLETRDFAKYPFLKESQQFMLQNHESVDRFLQSSAGKVAIR
                     YAVERVQRALSHAGQTIEIERIPLPSDSLGVRLEVSSYALARLLVSCGKERALIDRLT
                     RYEALRACAFLEGEDDGKRAYVASQIGFSLGRLSLPVSRYVEITAAMREERWRLVNRE
                     VEDGNVAIEPLEAEELLKEQIRVTLLSQLPLDVPQSLCEEMAPYVSRIQDAFQQKMLE
                     DFGEVEESCFPPCMQAIMSALSGGLNITHSGRFALTAFLHNIGMDGAQIIELYCRAPD
                     FDVSKTQYQVEHISGRGGTGTEYTAPSCAAMRTLGLCVRPDAGCEKVNHPLNYYRSRK
                     KGKKAKPN"
     gene            complement(5416..5592)
                     /locus_tag="PKM87_01170"
     CDS             complement(5416..5592)
                     /locus_tag="PKM87_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28707.1"
                     /translation="MDARVLDILSAVVSFIVLLVFLLVLPLFLEQGIAYLLAIVIFIL
                     TMSGAGFYINKTLS"
     gene            5703..6257
                     /locus_tag="PKM87_01175"
     CDS             5703..6257
                     /locus_tag="PKM87_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate synthase family protein"
                     /protein_id="HNQ28708.1"
                     /translation="MMSTPGDEREVVLRELEESVRALSLGMAVLLIPEVGSNIAYATP
                     TARDPSQVAAVEGRIVRLSGRAHPVGQVSFGASDHVARIVLTAMRFDPQVRCAANIRY
                     SPETVRILEEMLLEICSFDRSSEPPGVKTMDWGVASCCRDGVPDVIYDLGSVGKEPMI
                     RLLGENPGAVVKNILKVSARMNKE"
     gene            6275..6463
                     /locus_tag="PKM87_01180"
     CDS             6275..6463
                     /locus_tag="PKM87_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17e"
                     /protein_id="HNQ28709.1"
                     /translation="MGIKPTYIKAIGIELLNHHGNRFSNNFDENKQTITEVTNIESKR
                     VRNRVAGYITRKVNTKRR"
     gene            6477..7352
                     /gene="dapA"
                     /locus_tag="PKM87_01185"
     CDS             6477..7352
                     /gene="dapA"
                     /locus_tag="PKM87_01185"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNQ28710.1"
                     /translation="MFEGVIPAIVTPFLRNSTRDVDIEGLRSNIEFLLAHGVHGIVPC
                     GSTGESATLTFEEHEKVIEVAIDVADGKVPVIAGTGSNNTAEAIRLTKAARDMGADGA
                     LVISPYYNKPNRSGLVKHYTALADLDIPVIMYNVPGRTGQNLAPDLVSELACHPNIVA
                     IKEASGDIGQISRIIEGTLDEDFQVISGDDNITLPILALGGAGVISVAANVEPARMVA
                     MFESFCDGDLDAALDMHFTLSPLFRAMFIDTNPIPVKKAVELRGMAGGPPRLPLDELD
                     DEKTVKLAEVLSTYD"
     gene            7345..8106
                     /gene="dapB"
                     /locus_tag="PKM87_01190"
     CDS             7345..8106
                     /gene="dapB"
                     /locus_tag="PKM87_01190"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNQ28711.1"
                     /translation="MTNVVVCGALGRMGTTIGRLVNEAPDLTLVGGVDVRAGDLFGTR
                     VVEAARMDALLKELKPDVVIDFTVANAAVENVRTAAKHGAALVVGTTGFSSEQRAEMA
                     RAVEGHVPAVISSNYSIGVNIFWQLLRQAARMLPDYDIEVLEAHHRYKKDAPSGTAKT
                     ILQILEEEVGEREKLYGREGMRERGREIGVHVIRGGDIVGDHAVIFAGNFETIQVSHR
                     AYDRSVFAQGALMAARWVVGKKPGIYGMNDVLGLA"
     gene            8180..8353
                     /locus_tag="PKM87_01195"
     CDS             8180..8353
                     /locus_tag="PKM87_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ28712.1"
                     /translation="MTAVVDKEKCTGCETCVDECPAAAISMEDGKAKVDKDLCVDCGS
                     CVDVCPSEAIHLE"
     gene            8399..9415
                     /gene="asd"
                     /locus_tag="PKM87_01200"
     CDS             8399..9415
                     /gene="asd"
                     /locus_tag="PKM87_01200"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNQ28713.1"
                     /translation="MINVGVLGATGAVGQRFVQLLAEHPWFNLTALTASDRSAGKQYG
                     EVVNWRLDVPFPKNVADIRIRTTDVKSLKDVDLVFSALPAEVAAGVETACADAGLGVC
                     SNASSHRMDPLVPLVVPEVNPDHLALIDAQRDGGRDGFIVTNPNCSTIVMCLSLAPLR
                     KFSFGDVRVATMQAISGAGFQGVPAMGIYDNVIPYIGSEEQKMETETLKILGHLDGKA
                     VKPASLKVSASCHRVPVIDGHTMAVWIDINEPVEMVRDAFREYVPPISGLPTQPERSL
                     LYLDDQDRPQPRLDRNRGNGMTVSVGRVREGIRYVTMGHNTIRGAAGASVLNAELIHT
                     RKYL"
     gene            9428..9703
                     /gene="albA"
                     /locus_tag="PKM87_01205"
     CDS             9428..9703
                     /gene="albA"
                     /locus_tag="PKM87_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006325 - chromatin organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein Alba"
                     /protein_id="HNQ28714.1"
                     /translation="MLKDNTVFVGNKPVMNYVLAVVTQFNNGAQEVSIKARGKAISRA
                     VDTAEIALNRFLEGVGKKEIITSTEIIDTDSGKTNVSSIEIVLSAPG"
     gene            9951..10403
                     /locus_tag="PKM87_01210"
     CDS             9951..10403
                     /locus_tag="PKM87_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28715.1"
                     /translation="MRGVAIAVTVLCILSMVLPVVAANEEKYGYITIQNVDITLEKGK
                     ANIDVQYSLDEPTRLIVLLLGKQDLKNRILKVLNYEEAEVVEIEMNRANLVVEDVSYN
                     YGRGIYWFPAHQFNVLIPTLRITTPQISREFCSTKMVPNGIGYFDNPE"
     assembly_gap    10443..10542
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10829..11956)
                     /locus_tag="PKM87_01215"
     CDS             complement(10829..11956)
                     /locus_tag="PKM87_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF373 family protein"
                     /protein_id="HNQ28716.1"
                     /translation="MYQGRTLVLSVDRDDDIGFKASVNSPVIGRDACLAAANTLGLAD
                     PEDSDVNAIFQAIKIYDELLGRGEDVEVAVIAGNHMHMIEGDRRIAASLGDVVAQTGA
                     SNCIVVTDGAEDEFVIPIVQSKLPVSSIRRVVVSQMPNLEGTYYIIKKLLNDPKVARL
                     VLVPLGLAMLLWAVAYLVDMPQIATFIVIGAIGIYLLYRGFGVDEIFEGFAHALRTSL
                     TRGKFSFITYIAGILLVIVGVIMGLMNLLVYYAEIGLLLYFLIFMYGAVLWLALAGVV
                     SSIGIIIDNYFYDRVGLAKVIVFPFFIGAVGLITYGACIYAISMNNIPDFPYSTSTAV
                     QFIFYGTLGGLICALSGIGIQYMVNKYLASQRSGESVELVS"
     gene            complement(12017..12877)
                     /locus_tag="PKM87_01220"
     CDS             complement(12017..12877)
                     /locus_tag="PKM87_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HNQ28717.1"
                     /translation="MLNELIEKRKKILAESEQHKNRRNELNALASKGARERNTLNNQT
                     REYVEEAQKNKELRDKYNNDVQAIKDERNQINEQANAFFEEIESFKKEHGGMRNRGLK
                     ELQKQIEHLEFRQQTEVFSTDKERELIEKIKQMKESVREQEAELEQNKEIKAKISSAR
                     DLRKQASDLHAKVTEMAELAQKHHDLMVDFYRKADKSREEADAAHKNFVEAQESADAE
                     HKYFIACQKELRDYDKVISGLRKKTKKAKVTKEQKAVRKEAEHLFKMFRAGEKLTTDD
                     ILLLQRSKLI"
     gene            complement(13371..14528)
                     /gene="ftsZ"
                     /locus_tag="PKM87_01225"
     CDS             complement(13371..14528)
                     /gene="ftsZ"
                     /locus_tag="PKM87_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNQ28718.1"
                     /translation="MQSIINDAQKNFEIERELANTSSSIDDEFIGQPRIVIIGCGGAG
                     NNTINRLHHMGVSGAETIAINTDKQHLDMIQSDKRVLIGKSLTKGLGAGGYPDVGKRA
                     AEMARPTLEALLENADLCFITAGMGGGTGTGSAPVVAQIAKEQGAIVVGMVSYPFQVE
                     KARLIRAEEGLEALASAADSVIVLDNNRLKNFVPNLPLGQAFSVMDQLIGETVKGISE
                     TITQPSLINIDYADVRAIMSKGGVAVMLVGESKQQNKSESVVRECLSNPMLDIDYRGA
                     TGSLIHITGGGDLTLQDAEEIASSLTYELDPHADVIWGARVKNDMEGKVRVLAIMTGV
                     QTGKIMGSRVSYKTILEDLEQKKVSPKAPQLPRNRKARDQTAGGTLLEWVG"
     gene            complement(14540..14716)
                     /locus_tag="PKM87_01230"
     CDS             complement(14540..14716)
                     /locus_tag="PKM87_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HNQ28719.1"
                     /translation="MKRITLRLPDQQIEMLEQMVAAGEFPTVSEAVRDAVRQLIERRG
                     GRILKESDQVSFKV"
     gene            14923..15921
                     /locus_tag="PKM87_01235"
     CDS             14923..15921
                     /locus_tag="PKM87_01235"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNQ28720.1"
                     /translation="MNGQHLSKVFHGGTGIRAGKEAGAPLLDFSASINPYPPHLSFQL
                     DPFRLEHYPDDTYTELKGVIAGRFCRDVDEIAIGNGSIEIIRVLCSSVLSPGDSVEIS
                     PPTFGEYEFSARLAGASIGRGSGTRLRFLCNPNNPTGHLVERDEILSMLDRCEEDEAL
                     LAVDEAFIEISDDPMQTLVGIRHPSLFVMRSLTKSFAVPGIRFGYGFGNPDLVGMLEA
                     RRLPWSVNVVAEHLAIEAFLRYEELSISRERIRSERERLCRGLLDLPLEIVPSRTNFL
                     LLNTKGDVVPLVECLMRRGILVRDCHSFGLPNSIRVAVRTREENLRLLEALSECLR"
     gene            15909..16523
                     /locus_tag="PKM87_01240"
     CDS             15909..16523
                     /locus_tag="PKM87_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNQ28721.1"
                     /translation="MLALILAGGIGSRLALGEKPLVTIDGMPMIMYVIEAFESAGFDV
                     VAVLSRKTPYTHNWCRAHGISHYTAEGMGYVEDIAECADAHGITGPFFTSVADIPCLE
                     RDIVQTLHEVYRHSQKDALSSWIPRGLAERFGSRVAYIEKINGLDACPAGINILRGDC
                     MGTPQDETRVILDDFRLAYNINTREALDLVTRVIRARRNLGRNS"
     gene            16633..17865
                     /locus_tag="PKM87_01245"
     CDS             16633..17865
                     /locus_tag="PKM87_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HNQ28722.1"
                     /translation="MQVSREVELEGHIIDSGIMTRVFDKIMDMGGNFEIIVFNVGKRK
                     SDPSYAHLQVNADTENQLHAILSEIHRFGVRLLEIRDVTIVPAEGNRIVPQGFYSTTH
                     HPTTIKFEGNWIPVQDIEMDCLVVVDKRTMSAACTPISKLKKGDLVVVGEEGVRVNPP
                     ERPRHISTFEFMQGTVSPERPSETIIARIATEIIELKKKGGKIAVVGGPAIIHTGAAL
                     ALASIIRKGYVDVLFAGNALATHDIEYNLFGTSLGMDLSTGKPVTGGHKNHFYAISEV
                     MRAGSIKSAVGKGVITGGIMYECVQRDVPYVLAGSIRDDGPLPDVITDSMEAQDRMRE
                     QVRGCNMVLMIATLLHSVAVGNLLPSYVKTICVDINPSSVTKLMDRGTLQAIGVVSDA
                     GTFLPLLEKQLELQSGSA"
     gene            complement(17862..18845)
                     /locus_tag="PKM87_01250"
     CDS             complement(17862..18845)
                     /locus_tag="PKM87_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosine biosynthesis radical SAM protein
                     RaSEA"
                     /protein_id="HNQ28723.1"
                     /translation="MNIPVERPLAAWTGSDRIRDQVMSALTIILKTGGCAWNRCRMCS
                     YRHERYGELGQGGLEERLLSQVSWIRNNFCLDEIEAVKIYTSGSLFDTSEVPVRARDA
                     LAEMVRGKIVIAETRPEYVSDDHLSAFLSAIDSGAHDTPLYVAVGVETSNDMVREKCI
                     NKGFSWDDFVKAAARARSSGAGVKAYLLAKPPFLTEREALDDMISSIHDLDGLADMIS
                     LNPCTVQRNTELEYYWKQGAYRTPYLWSILSILAESPVHLSCDPLGGGQERGPHNCGT
                     CDRTLVKGIRDYSLSADRKLLSALLSTDCPCKEEWKFVLEEERPYCMPLTR"
     gene            19265..20380
                     /locus_tag="PKM87_01255"
     CDS             19265..20380
                     /locus_tag="PKM87_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28724.1"
                     /translation="MTRIRQIIRAKFRVVILMSVFLLLLAGVSAAYELSIYAPMNVQR
                     GMPLVVNGTSNLPAGISVDVILSRSEYTTEEIARETVTLQGPKEFNVVFDTQELSKGQ
                     YKVEVPGVQGYAFLGDSTTIRVVQIIDRTDELTIKSPRTQEMDGSLELRGIIPHMRNS
                     GIQVQVIGPENEVVFGPEYIATQYDGSFSKEIRIDEPGTYEASFTDTKGYIGTYPFEV
                     TPKFEPTPIPTTPEPRMVAVTATAHSSNDGPAYFVVTGGSGKAKISTSAGIDWVVEYT
                     DARGAIQKINNKGQIDPEEFTIDSGGETIYLKVYPYRFSDEGEVTIFAEGADRVGVAT
                     EVPPAFAATATPTPKAPLPGLLALFAVLALGLRRGFR"
     gene            20456..20540
                     /locus_tag="PKM87_01260"
     tRNA            20456..20540
                     /locus_tag="PKM87_01260"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20490..20492,aa:Ser,seq:tga)
     gene            21492..21833
                     /locus_tag="PKM87_01265"
     CDS             21492..21833
                     /locus_tag="PKM87_01265"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ28725.1"
                     /translation="MNRTVLHIAEKAGYYARMGMETATTGNYTNALEYYDRALKEMPG
                     YAAIWREKGNCLDSMGRCDEAVRCFDQALQLDPGDAEAWFDKGLTLKKLGNEEEAFRC
                     MSRGVDLEIGV"
     gene            22086..22889
                     /locus_tag="PKM87_01270"
     CDS             22086..22889
                     /locus_tag="PKM87_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ28726.1"
                     /translation="MNSELTQTLREWCKCRGIPVMGVANASRWEKPPFSPWMHESFYP
                     RSIYPDARSVIVIGLPVHLPVLETTPSIYYHELYQTLNRLLDEHTYQMAEWLNQEGYP
                     SLFVPRDGYGSLEVLLNKPVAFFSHRHAAYLAGLGTFGVNNMILTPGYGPRIRFGSVF
                     TAAELPPDPIMEEDLCIHCMRCVEMCPSRALSGEGYPEGITDKKACAEYSAGLFRRSI
                     SPCGICIKVCPVGADRAAFHREDADIYQKGHTPAGFASAWDHVRAYGGK"
     gene            22972..23202
                     /locus_tag="PKM87_01275"
     CDS             22972..23202
                     /locus_tag="PKM87_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HNQ28727.1"
                     /translation="MCIAVPAEVVEIKDGNIGVVDYGDLKQEVRLDLVDVKVGEFVLV
                     HVGFAIQKLSREEGLETREIFRQVYAAMQESE"
     gene            23358..23537
                     /locus_tag="PKM87_01280"
     CDS             23358..23537
                     /locus_tag="PKM87_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28728.1"
                     /translation="MDKTGVASLIAGIILVILGGYGVWIFLPDVIVFLKGVIGILAIL
                     IGIFLLIFGAILVKD"
     gene            23760..24593
                     /locus_tag="PKM87_01285"
     CDS             23760..24593
                     /locus_tag="PKM87_01285"
                     /inference="COORDINATES: protein motif:HMM:NF039195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HNQ28729.1"
                     /translation="MSASENPVTGAVSRSMMHAYHQAFPPLDNSSASLNSTANATYEM
                     NDTAVYENPGDENLSLRDILISMDDEGTLDEELLLTLLAEREWNMSVGEEEIWVEPEP
                     TPDPNAWKEYSNAKWRFSLIYPPTWEVKEGSSSSPAATFSAPVEAECSSKTTECYQYV
                     ASLTVSIDQKPETIVLEDYFNKKVSKLQKDLAITATSKSAPTTLSGNKAYWIEYYTRD
                     SRGNPSKSYMQYFTILDKKVYILTYSGPFSTAENVYSRNKPEVEKMIKEFVVEREYKT
                     L"
     gene            complement(24851..25105)
                     /locus_tag="PKM87_01290"
     CDS             complement(24851..25105)
                     /locus_tag="PKM87_01290"
                     /inference="COORDINATES: protein motif:HMM:NF013006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFYB/HAP3 family transcription factor subunit"
                     /protein_id="HNQ28730.1"
                     /translation="MPKPVKKAPAKKKAKAAHTSQFDLPLAPVIRIAKRSGAVRISMG
                     GTRAIVVSTEEYIAAIAREAAHSAASDGRKTIRAEDIEKY"
     gene            complement(25426..26076)
                     /locus_tag="PKM87_01295"
     CDS             complement(25426..26076)
                     /locus_tag="PKM87_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNQ28731.1"
                     /translation="MTKEKSGRNKGKKGVFEFIFPIEHDFEAMLVEQAEKTLECVYSL
                     VQWLKKTPTDDPQQLMQLEMQLDAMRYDMEDKLMASFSTPFDRQDIYSLSRQMDYIVN
                     YSYETAREIYAFEVHPDDAILGMAQALLQGTKCTAAGVRAMGRDKKRVEELIRSARSA
                     MRMIDDIYIQSMASLLRTDNPMDAIRKREVYHHLRDAGRALRTTVDILHHAVVGLS"
     gene            complement(26073..27122)
                     /locus_tag="PKM87_01300"
     CDS             complement(26073..27122)
                     /locus_tag="PKM87_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNQ28732.1"
                     /translation="MGTLSMIGFAWLAILLAFLFTFTNGFQDASSVAATFISSRSATP
                     RQGIIFVAAMDFMGALFGGSAVAFTISGLLALEADTHMVPVVLAGLLAALVWNLGTWW
                     TGLPSSSTHGLIGGLVGAGIAAGGLGGVHWGFLEFLAPPHEMTGLTKVVVFLVVSVLL
                     GFMAGYLMRKATRVLLRNAKRMVIRDIVRLNWMAAAVMAFSNGANDSQKQLGIISLVL
                     LSAGLSPSLDIPMWARLGCAALLGAGTLSGGWRIMTTLGRKIFRIEPIHSFDSQVSSG
                     SVLVASTLAGAPVSSSHIISTSVIGVGAAENIRKVQWSVGREIVLVMLVTIPATMVLA
                     FGISLVLFPLTGSIS"
     gene            27336..28532
                     /locus_tag="PKM87_01305"
     CDS             27336..28532
                     /locus_tag="PKM87_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ28733.1"
                     /translation="MTHKVFAKAPTAMKLILLLGVVSLLGDIIYEGGRSVTGPYLLFL
                     GASAFTVAWVSGLGEFIGYAIRLVSGYFADRTREYWTFSLAGYLMIGAIPLLVYTRSW
                     EAAVVLLLIERLGKGIRSPAKDTILSHAAHQVGRGWGFGIHEAMDQIGAVAGPLVFAA
                     SLLLLGGYGEGFALLTIPFVLLIAALVLAWRRYPDPASMEESALSFGYTPKDPRVLLP
                     YGAFTVLTMAGFLVFPLMAYHFSAAAVVPDPQIPIFFAVAMATDALAALLMGRLYDRW
                     GLPVIIAIPIANIPIAPLAFSSGYFGALAAAVLWGVSMGGQETVLRAALADFTSIGKR
                     GFAYGIFNTLYGASWFAGSLVFGLLYYVGAGAICAFSIFMQVAAMGAFFLLWRCAGRS
                     SRGYSP"
     gene            complement(28711..31929)
                     /locus_tag="PKM87_01310"
     CDS             complement(28711..31929)
                     /locus_tag="PKM87_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ28734.1"
                     /translation="MARVHTCTRGKIPFFLFAEGCGIPWAHYFIILAILFLICPVSPA
                     DLSSPERKEVLYLNSYHRGQEWSDPITDAIEDAFLNSSAPINLHVEYMDSKRFTDDEY
                     VRTLFSLYAHKYRNTRFDALIVSDDNAFKFIMDHRDSLFSGTPVIFCGVNYYSPDMIG
                     ERDGITGVVETFDVKGTLDAALSLLPGTKEVFVINDASLTGAANKKVISDIAPLYAGR
                     LNFTYLEEYTMAELQEKVSSLPDGSVILLMTFNQDRAGDDFSYLETLEYLYPHSRVPI
                     FAIWELYIGHGIVGGMLTSGYEQGRAAASLALEVMNGTDPSEIPVVVGPENHYVFDYR
                     EMQRFGLDVSSLPPGSRILNEPSALVPIPEYLVWAAMISGTCMVLLIAVLLHSNARLR
                     RIRNALDSSERRYRAVVEDQTELIFRTAPDGRIVFANDAFCQFFQRESEEILAPAGDP
                     PIPAGNMDALTKHFQSLSLDNPSGILVDMVILPSGSPRWIRWSNRAIYDEQGVLKEYQ
                     SVGRDFTDLKNAEKELLDYQKTLESEVAARTCELASAYSGLKNEIADRIKVEELLAAE
                     KELLSVTFNAITDGVISTDLQGCVRFMNRAAQEMTGWAVEVAEGQTLDRVFPVVSGGR
                     DSDPDLIAEMHGQCGGLEIQRDLTLLPRNRGELPVSVTVTPIRDRVSRIMGVVIVFRD
                     TTMTQKYEQEQLRSQKLEAIGNLAGGIAHDFNNIVTGILGNISLAKLETPGDTPLYTN
                     LSKAEEAIYRARSLTQRLLTFSRGGAPVKRVVAIGPLVRETTEIMLTGSRSKPTFSIP
                     PDAWSVEADPDQIAQVIQNLVLNADQAMPEGGIIGVTVSNTTLGEGSTLPLPPGHYVA
                     ISVTDQGQGVPEDIQSQIFDPYFSTKAEGTGFGLSAALSIVKKHGGIIDLHSRHGEGA
                     CFTVYLPAATSVKPAESSQEKILPSGSGRILILDDEAGIREILHTILTRQGYQVDEVA
                     EGREAIARFRDCMEGGTPYDLVIMDLTIPGGMGGLGAFRQMRDLEPGVIGIVSSGYST
                     DPVMAEYRSYGFSGVIQKPYRLEEIVNLVARLIKERREKEER"
     gene            32294..32584
                     /locus_tag="PKM87_01315"
     CDS             32294..32584
                     /locus_tag="PKM87_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28735.1"
                     /translation="MITLMGIFKSVDGGITRPRLKDELEYAIWKITGLSIPYNEHVIP
                     HLSQEIARKTGEDPGEVSMRLAAQIKEIIWADLQYQFRDRAPCKKAVEHITD"
     gene            32729..33163
                     /locus_tag="PKM87_01320"
     CDS             32729..33163
                     /locus_tag="PKM87_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNQ28736.1"
                     /translation="MSFEECAVFANQHPVCAVATVDGDQPRVRFFLMWYADGTGFYFH
                     TGKMKQIYRQLQQNPKVELCFYDPTPPEQGGGKMLRVTGRVEWKREQALVNRLLAERP
                     WIRSLGTDIDQFLAIFRVKGGEAWFWTMADNLKEDRIPRYEF"
     gene            33221..33691
                     /locus_tag="PKM87_01325"
     CDS             33221..33691
                     /locus_tag="PKM87_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28737.1"
                     /translation="MKDRNLCIGGLFLIIAVVVGGTVLTSGCTEQTSTPKENPFPGIW
                     VYHGKIGTYNATIVFTFNENMTGRYDMLVPDTTPPYMNSMEFPWESDADQLFIGSSSE
                     KQHLDIRYYPDRDRIVVLADEESGIFIGEEFVTGPFGWEFFRVIEGEGTPGTSS"
     gene            33788..34123
                     /locus_tag="PKM87_01330"
     CDS             33788..34123
                     /locus_tag="PKM87_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28738.1"
                     /translation="MTRLQILLALAVFTLACAGCIDEELSPVVGTWEWSDKKGYTERY
                     TFYANHSFFAEALGAGFEGTWEELSFGHYEVTYWNRNDPGRSEVFRERVLYDAETDRV
                     YFPGHERVG"
     gene            complement(34319..34504)
                     /locus_tag="PKM87_01335"
     CDS             complement(34319..34504)
                     /locus_tag="PKM87_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HNQ28739.1"
                     /translation="MAAGKFEVYTDKAGKYRFRLKASNGEIIAVGEAYSSKSACMNGI
                     ESVKKNAATATIEEVKE"
     gene            complement(34615..34689)
                     /locus_tag="PKM87_01340"
     tRNA            complement(34615..34689)
                     /locus_tag="PKM87_01340"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34651..34653),aa:Glu,seq:ctc)
     gene            34880..35992
                     /locus_tag="PKM87_01345"
     CDS             34880..35992
                     /locus_tag="PKM87_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNQ28740.1"
                     /translation="MAGLEEQIKELEEELQKTPYNKATAKHIGRVKAKIARLRDEAVT
                     RAMRAGGGGEGYSVKKSGDATVVLVGFPSTGKSTLLNALTGTKSEVAAYAFTTLTVVP
                     GALEHKGARIQILDIPGLIAGAAMGKGRGKEVIGVVRGADMIVILVDVFNANHVDVLM
                     KELYDAGIRINRPRPDITIKKTALGGVRMNHVGILDLDVEEIRSILSENKMMNADVLI
                     RGNATQDDVIDAMLANRVYIPAFVAVNKVDLIDDTARKAIEEEILARFGDAPLLISAH
                     TGYHMEEMKDEIYSRLGFMRVYLKPQSGAADLEEPLIIRSGSTVEDVCRKLHRDFVDR
                     FRYARVWGHSVKHPGQRVGLPHRLREGDLLTIIIEH"
     gene            complement(36013..36528)
                     /locus_tag="PKM87_01350"
     CDS             complement(36013..36528)
                     /locus_tag="PKM87_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNQ28741.1"
                     /translation="MKILVVIPPDQFRDEELAEPLAAFQNAGISFEVASTRRGTCKGM
                     LGTQAMANFSLEEVEPGEYAGIVIVGGSGSPAHLWDNEILINLVKYFHKNRKLVAAIC
                     LSPVVLAQAGILKERTATCYMSPASKREIIKGGAHISEKDVVVDDSIITANGPAAAKE
                     FAGAIIHHLKG"
     gene            complement(36638..37780)
                     /locus_tag="PKM87_01355"
     CDS             complement(36638..37780)
                     /locus_tag="PKM87_01355"
                     /EC_number="2.1.1.213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004809 - tRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(10)-N(2))-dimethyltransferase"
                     /protein_id="HNQ28742.1"
                     /translation="MKYIEAEEGNTRFFIPVQDPSHPFPPASAPIFYNPRMELSRDAT
                     VVLVSLLSPSQYLDAMAASGIRGLRIASECRVPVTINDRDRGAFALIRRNAGLLGLPV
                     EVLHSDANVLLSGRCFEAVDIDPFGSPAPFVDSGIRGAGRYLFVTATDTAPLCGAHKN
                     AGIRRYFAQAMNNEYHAETALRILLGFLVREAVKYDRGIEPLFCFSREHFVRVHVRLS
                     RGAKAADRTVARMGYVMQCHSCPFRSEETGLLPEPGFCPECGAGLTPVGPLWTGSLQD
                     REILEKMEEAIPAFRLAKSAQLLRLVNTCRQEPDISFHYDYHVLSRHFRLSPPPMEGV
                     IRSLVAGGYAAARAHYSGTALKTDAPLDVIREVLLAGRSGIDDQGI"
     gene            37840..37914
                     /locus_tag="PKM87_01360"
     tRNA            37840..37914
                     /locus_tag="PKM87_01360"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37875..37877,aa:Arg,seq:cct)
     gene            37984..38844
                     /locus_tag="PKM87_01365"
     CDS             37984..38844
                     /locus_tag="PKM87_01365"
                     /EC_number="2.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HNQ28743.1"
                     /translation="MQEEGAILPYPPYLRVIRPLNALVAGCAAIIGYFLAAGTLVPGI
                     AVLFLVVFLVTGAGNAINDYFDVEIDRINRPDRPIPSGEASAKGVLVFSILLFLAGIA
                     LSFLTNIFCIVIAVANSLLLAWYAAALKRTPGAGNAAISYLTASIFLFGGAYAGPDGL
                     QLVSPVALITFFAMFAREIWKDAEDVLGDVASGADTLPIRIGVYPAICLGFVFLVGAL
                     LASLVPVLWWGPYYLAGIACVDILILCVGIRALGCKTAACLKKSRVTTLLKYAMFASL
                     AVFAVSAFLL"
     gene            complement(38998..39702)
                     /locus_tag="PKM87_01370"
     CDS             complement(38998..39702)
                     /locus_tag="PKM87_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HNQ28744.1"
                     /translation="MWQSLVFLIGEITIVVIIGIFFFALILVALSIYSINTGRLYFPR
                     FLKAGMVLVEGLAKSLFRLFGLEDSEVLTFFINLHNTMNKKSFSEIPVTERAIFLPQC
                     LRSSRCPAHLSPEGLKCRSCGECTIGFWRKILEAMGYRVFIVPGSSFIKRMVKKYHPR
                     AILGVGCLSEVKEGLEMSDKMGLVAMGVVTLKDGCVETLVAWDKVMEVATLGVPPAGI
                     PPEAKEVNSWPVAEDY"
     gene            complement(39735..40784)
                     /gene="mtnA"
                     /locus_tag="PKM87_01375"
     CDS             complement(39735..40784)
                     /gene="mtnA"
                     /locus_tag="PKM87_01375"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNQ28745.1"
                     /translation="MRAVETLHWDDEEVALTLIDQTRLPGHFRIVRCRTVDRLITAIR
                     RLEIRGAPALGVAGAYGVVLASRKIREREHFRFMEMLKAEAARITDARPTAVNLSLGV
                     EIVMRVVESAHSVTEAREKALRAAREFTAADSRACHELGDYGAALIGKRCTVLTHCNA
                     GALACSEWGTALGVVRSAVEAGSDVEVIACETRPLLQGARLTAWELQRDGIPVTVIPD
                     SSAAFLMKLGEIDAVILGADRITPDGVFNKIGTYMHAVCASHHGVPFYVAAPLSTFDP
                     HRIAREVTIEQRDRNEVAFCGSTVTVPDGVGVKNYAFDLTPHQLVTAFITDIGVISPP
                     VDWNEIEAARKDRGL"
     gene            complement(40781..42064)
                     /locus_tag="PKM87_01380"
     CDS             complement(40781..42064)
                     /locus_tag="PKM87_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HNQ28746.1"
                     /translation="MSDVVIVGGGVTGIQAALDLANHGIEVHLVEKEPSIGGHMAMLD
                     KTFPTNDCSMCILSPKMMDVLRHPRIHTHTCAKVSAISGKAGQFSVTITKMPRYIDEE
                     RCNGCGDCIDICPVEVYNRFDAGIGVRKAIYKPHQQIVPDIVVKDPEHCIECGLCYDV
                     CGREAIRKEDTERKITVNAASIIIACGYDVFDAGNKEQFQYLRYPDVITSLEFERMMN
                     ASGPTGGKVRRLSNGKTPGSIVFVQCVGSRDMQIDRPYCSGVCCMYAIKNAMLIREKY
                     PETDVTILYMDVRAYGKGYEEYYQRAIDAGVRFVRGMPAEILEQEDSLFIRIENTENG
                     EMLELRPELVVLSVGLQPPADAGEIATLFDIPREPTGFFRPLDDKIETVATVRPGIYL
                     AGTTTQPRDIPDCVAHAGSAAMRAFIDATREGYPE"
     gene            complement(42057..42548)
                     /locus_tag="PKM87_01385"
     CDS             complement(42057..42548)
                     /locus_tag="PKM87_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNQ28747.1"
                     /translation="MAVGRTRTAETFVAENATVLGDVKLGLGVSIWFGAVVRGDRDKI
                     VISDRSNVQDNAVIHTSKGFPARIGEDVSIGHGAILHGCTISDRVLVGMGAIVLNGAV
                     VGEDCLIGAGSVVTEGTTIAPGSVVVGVPGKVIKQVTEAQRQHILQNARNYWEMAGSY
                     LHV"
     gene            42579..43037
                     /locus_tag="PKM87_01390"
     CDS             42579..43037
                     /locus_tag="PKM87_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine adenylyltransferase"
                     /protein_id="HNQ28748.1"
                     /translation="MKVMVGGTFDPLHDGHKRLISRSFELAGNGGEVTIGLTTDIFAS
                     RKSHPVRPFNERMESLERFIRENRWNAAWSIEPLNDRFGSALVAEFDALVVSEETLPI
                     ARDINRIRREKGRSKVDIHQITCVLADDGKWISSTRIYRGEIDDHGRLLT"
     gene            complement(43380..43988)
                     /locus_tag="PKM87_01395"
     CDS             complement(43380..43988)
                     /locus_tag="PKM87_01395"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ28749.1"
                     /translation="MNYRVFLLVALCISGIGVSCATSAPPLEAIDAYNHGIDLAVEGN
                     FSAALGSIDAALSMDPNFTVAWITRAGILNAMGDYNQSLEASDRALAIDENQAEAWTN
                     RASALISLGRNEEALEAADRAIELDPRLSEAWIDRSTALSNLGRTEEADEAVQMAMFL
                     IGELENSPVPQTTTAKAPLHWLLVPLGTGAALGFYSKKRYIP"
     gene            complement(44086..>44443)
                     /locus_tag="PKM87_01400"
     CDS             complement(44086..>44443)
                     /locus_tag="PKM87_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28750.1"
                     /translation="VLDIKPYVPGMYPREGVRIPGWMEKIMTDLGGNCQHDNGAGPGL
                     FTVNESELSYTGDLCDQSGNRGVKPLASLSCRMPITLWFFWGTAWCGSFPGGPMHTKK
                     LLFGSPFSNPGRWTIP"
     assembly_gap    44444..44543
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            44961..45200
                     /locus_tag="PKM87_01405"
     CDS             44961..45200
                     /locus_tag="PKM87_01405"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNQ28751.1"
                     /translation="MIIVNIPVTELRAHQKARIVGLVGGRGFQNRLRSLGIREGKEIS
                     VLVSQPFGGPVVIEIDGRATTIGRGMARAIIVEQA"
     gene            45201..46916
                     /locus_tag="PKM87_01410"
     CDS             45201..46916
                     /locus_tag="PKM87_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HNQ28752.1"
                     /translation="MKKIVLMGNPNVGKSVVFSRLTGARVVSSNYPGTTVDYYKGEML
                     LEKSRVEIIDAPGTYSLTPTNKAEEVAAEMLNEADLIINVVDATNLERNLYLTLELIQ
                     TQKPLIIALNLWDEAKNTGIVIDHRALGTLLGVPVIPTIALSGEGVQNLCRHVPRASA
                     GKIFDFTRDEDKWAQIGAIIKQVQQVHHRHPTIGDRLSLASIKPVSGIPIAIMVILIS
                     FWIVRFIGEALINWIFDPLFELYTPIINAFSQWLGPGLAQDILIGKLIDGQIDYVQSL
                     GLLTTGLYVPIGMVLPYIVAFYFVLAILEDSGYLPRLATLSDSLFHRMGMHGHGIVPL
                     FLGIGCNVPAILATRCLETRKQRFISATLVSISVPCMAQSAMIFAILGPYGVGYILLV
                     FLVLLTVYLTIGLLLNRVVKGMSPELCLEIPPYRVPNPAVIVKKTWMRVRGFLGEAIP
                     YLFLGVLLVNLLFAIGFLEWVGAVCAPITAGMLGLPNEATTALVMGFLRKDLAVGMLI
                     PLDMTPEQLVIAVTMLTMYFPCVATFAVLFKELGIIDLAKSAIVMICAALVVGTILRI
                     ILLGL"
     gene            46927..47346
                     /locus_tag="PKM87_01415"
     CDS             46927..47346
                     /locus_tag="PKM87_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNQ28753.1"
                     /translation="MRKKTIEEYIELIYSLEKRDGHAHTGLIASEMNIKPPSVTQMLQ
                     KLKREGLVCYQSYSGATLTPKGNEMARRLMEKHRVIEEFLKMIGIEQNVAELEACQIE
                     HHVSINTARQVERFVRYLKKKNDDEGWLDEFKKTVMI"
     gene            complement(47465..>47822)
                     /locus_tag="PKM87_01420"
     CDS             complement(47465..>47822)
                     /locus_tag="PKM87_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28754.1"
                     /translation="SSLCLHHVSPADRQEVARKASSVLHAGGRFICGDIFRAAEDWEE
                     RILTASWVEGMKREGAPDEVIEGMLRQRAGRLPEISTITDFRRSLLAAGFERTWVPFT
                     HGFVGLAAGMMSTHSK"
BASE COUNT        10618 a        12844 c        13209 g        10951 t
ORIGIN      
        1 ttttacttgc ccatgagggg aatattgcag tccgggcagg tctggctcct gcagggaacc
       61 ccgcgtgtct ttgggtactc tttcttgcat ttcggacaga tgcaggcgtc aggagggact
      121 cctcttcgct gcatccccat tcccggtcct ccgttcggca tttcgttcac cacgaatgaa
      181 atgatgtgct ggcgatgtgt taaaccatat gaatggggca agggggcggg tgtgtatgga
      241 ggcgacaaaa tagattctta tttggaccgg gaaatcagtc tattccagtc ccatagaggg
      301 tgcatctctt aaatcattac ataccggccg agcatccctt cgtataccct ggccgcgttc
      361 tccagggact ctatggtgac atgctcgtca attgcatgga gtgtccggat ctcgcctgga
      421 ccatactcaa cgacagggaa cccggcgttt cgcaagtacc gggcgtcgct tgccgcccac
      481 tggacgatgg gccgggcggt ggcagagtgg accttctcta tctcccgaag gaggcgggtc
      541 acgagcggcg agtccggagg cgtgatggta ggatccgagc tattggtcac tgatatttct
      601 ccgcgggggg catgacggcg gatctcctcc aggagatcag gaaggctgca cccccagggt
      661 atgcggaggt caagctcgag gctgcactgc tgtgctacga tgttcgcctt ctcgccgcct
      721 tcgatcttcc cggggttgta catcacgtgg gacaggactc ttttcgtgtc ctctattccg
      781 aagatctctc ccaggatctt tgacgattcc ccgataatcc cggagagggc gggatctgcg
      841 gggctgaatt cccgcttgtg gagcagcctg acaaactcga ggagcgagaa cgcctccatc
      901 acggcgctca ctcccttcgc aggatagaga gagccgtgcc cgggctctcc cctgaacctg
      961 cagcagaggc gcagtaagcc cttctgaccc acgtttgggg agagcgacgg tgtcggctcg
     1021 gcgacgatcg tatcgcaggg gaccaggcat ttgctggcga ggagtgcctg gataccgtgc
     1081 gttccgcttg tttcttcgtc gcagacgaat gcgaggtctg caggaagttc cctgccggaa
     1141 tcaagccagt tcctgcatgc ccagaggacc gaggcgcaac cgcctttcat gtcggtagtc
     1201 ccacgccccc agacctttcc gtcctcaata gtcccggcaa agggatcatg ggtccagccc
     1261 tcatcaagtg ccgggaccac gtcgacgtgc ccgcagaaaa gaagtttccc gtttaccgtg
     1321 cgggagacaa ggttgtcctt tcctcccggc ccccggatga cctgcgtgac gattccaagc
     1381 gaatcggtga aattacggat atattcaatg acatccctgg tattcccagg aggattttcg
     1441 ctccggatcc tcaccaggtc tgcgcagagg tcggtaacat tcataaaaaa atgggtcagg
     1501 ctttcctttt gaattcctcg aacagggccg aaagggaggc atcactgtag agagtcttca
     1561 aaaactcagc gttgaaaaac tcctcgatga gggagcgata gacatgcggg cgtatcgggt
     1621 ccttgctgtc ccggtcaaag actaatcgga aactgatata gcgcgcctcg tcgtcctcgt
     1681 catacaccct ccgccagacc gtcggaagcg tctcgaacct ctttgggtgt tctctcttca
     1741 gccagttgat gaacgccgga aacctggggc ggtcgccaag cttctcctgg tcgaccctgc
     1801 aacgcaggta ttcgccggcc atcatcaccc ggggcgactt gtagtggtag gccatgaggc
     1861 tgatcacata atcgagatgg gcaagggcgt ccatgtaata aaaggcaagc acgggatgcc
     1921 acatggtctc gtcctgcagg ataagggact tctcgtacag gtgctcaagt acgtgcacgt
     1981 attcagcgtc cgaactcatg gtagatcctc agacgcgggg gagtaaatat attccggaaa
     2041 cggtatagtt tccttttttt gggaaataat ggcgaaaata atccttttac tggaagaatg
     2101 gcgcccttcg gcggaccgct tcgcggaaga gctcctcgag ttcctcgccg tattttccgc
     2161 ggatgtccac gtggttgtcg gtccggagaa gacagggctt cagttttcca tccgacgtga
     2221 cccgaagcct gttgcaatag gcgcagaact cggtgttgtg aagaggtctc accacctcca
     2281 cctcggcacc gtcaatgcag tacttcttcc tgtggtgcat gcggcgggtg aggatctcct
     2341 gcgaattgct ggcaagccat acctcgaggt cttttaactc gtggtggttg gtgcagtccc
     2401 ggaactccat cagctctatc acctgcagga tgagatccct gttcccccgc acgaacctga
     2461 tgaactcgtc aacctcgtga tcgttcatcc ccttcaggag caccatattc agcttgatgg
     2521 gagtaaggcg tgcctctatc gctgcatcga tcccggccag cacgtcgtca aggcagtcgg
     2581 agccagtgat cttcatgtag gtggcgcgat caaggctgtc aaggctcacg ttcacccggg
     2641 agagacccgc atccgcgaga tcatgtgcca tgtcggcgag gagcgttcca ttcgtggtca
     2701 tcgagcactc catgcgttcc gggacggcgc ggacgatatc ggcaagatca gcgcggaggc
     2761 agggctcccc gccagtgaac ttgacactgc cgatatcgaa cttggccgca accttcatga
     2821 tttctgcaag ttcctccaga gagagagggg ccttcggatc cttttcgccc tctgcgtggc
     2881 agtagatgca atgaaggttg catgcgggcg tcaggcttat gcgcaggttg ctgaccggcc
     2941 ggttataggg atccttcagt ttcactccgg caccccacga aattccttgc gatgatatag
     3001 atctcgctgc tgcctctcct tgaagcccgg catttgaaag tcttcaccga gaggaaatac
     3061 tgccgcatct ctttcagaag tgcctggaag tcctctccct ggaacgattt gaggacgagg
     3121 tttccacccg gcttcagcac gtcgcaggcg aatgcgagaa cctcctgccc aaggccgatt
     3181 gcccttgcct ggtcatagct tcggtggcct gagagtttcg gagcggcatc ggagatgatc
     3241 acgttgacgg tcccgagaat ctttcctgcc tcatcccgca ggctccggtc agtgagatcg
     3301 ccgacgagtg tggtgacacc ctcgagcggg gcaatagggg agagatcgat cccgaggatc
     3361 cttccccccg tgagtccccg tgcaacctgg agccagctcc ccggggatgc ccccaggtcg
     3421 aggacgttgt cacgttcccg gatcaggtga tagcgttcct gtatctcctg cagcttatat
     3481 gcagccctgg accggtatcc ctctcttact gcccgcagat acgtgcgatc ctttccccat
     3541 tgtgaaccca tagtaatatc caccgtcgaa ataccgggaa gatcggatga aatcagatat
     3601 cattaaaacg ttgatggcgt agtttataat tacatatata cggggtgtac gatgttaaaa
     3661 gcaacgattg atgcagatat tcttaaagag tccatcgacg ccatctcagc cctggtcacg
     3721 gaatgccggc tccacaccga cgagaacggg ctttccaccc gggcggtcga taccgccaac
     3781 gtggccatgg tctccctcac actgaagaag gaggcgtttt ccgcatttga atctacgaaa
     3841 ggcgagatcg ggctcgatat cgccaagatg aagaatatct tcgggatgat gggaaagaac
     3901 gatgtcatca gcctggaact cccagaggac ggtcacaagc tgaaggtaag tttcgaggga
     3961 taccggtact ccatcaccct gcttgaccca aacaccatca ggaaagaccc gaacccgccg
     4021 aacatcgatc tccccggaaa agtggtcctc tccgggaacg acctcaacaa ctccatcaag
     4081 gcagcagcgg tcgtctccga caagatcgcg ctcgggatag acccggccca gcagctcttc
     4141 ttcatggcag cggaagggga taccgaccat atcaggagag agttcgggaa gaacgaggtc
     4201 aatttcctca acgggatgga agcccgttcg ctcttctcgc tcgattacct caaagacatg
     4261 ggaaaggtga tgagcagggc cgaggaggtg gaagtctcgc ttgggatcga tcacccggca
     4321 aaatttgcgt tctatatcgc cggggggcac gggtatgtgg agtacctgct cgcaccccgc
     4381 attgaggcgg attgatgcag gtctcccttg agacgagaga ttttgccaaa tacccttttt
     4441 tgaaggaatc gcagcagttc atgctgcaga accatgaatc ggtcgacagg tttctccagt
     4501 ccagtgcggg aaaggtcgct atcaggtacg cagtggagag ggtccagcgc gccctctcac
     4561 atgccggcca gacaatcgag attgaacgaa tccctctccc ttcagacagt ctcggcgtga
     4621 ggctcgaggt gtcatcctat gcgcttgcga ggctccttgt ctcctgcggg aaggaacgtg
     4681 ccttgatcga tcgcctcacg cggtacgagg ccctgcgtgc ctgcgccttc cttgaaggag
     4741 aagatgatgg gaagagggca tacgtcgcat cccagatcgg cttctccctt gggcgcctgt
     4801 ccctccccgt cagccgctac gtggagatca cggcagcgat gagagaggag cgctggaggc
     4861 tggtgaatcg ggaggtggag gatggaaacg ttgcaatcga gccccttgag gcagaagaac
     4921 tgttgaaaga gcagatccgg gtgacactcc tctcccagct gcccctcgat gtcccgcagt
     4981 ccctctgcga agagatggcg ccatatgttt cccgcatcca ggacgcgttc cagcagaaga
     5041 tgctcgaaga cttcggcgag gtcgaagagt cgtgcttccc gccctgcatg caggcgatta
     5101 tgtcggcgct ttcaggtgga ttgaacatca cgcactccgg ccgttttgcc ctcacggcct
     5161 tcctgcacaa tatcggaatg gatggtgccc agataatcga actgtactgc agggcacccg
     5221 acttcgacgt cagcaagacc cagtaccagg tggagcatat ctccgggagg ggcggcacgg
     5281 ggacggaata taccgcgcca tcctgcgcgg cgatgcggac cctcgggctc tgcgtcaggc
     5341 cggatgccgg ctgcgagaaa gtaaaccacc ccctcaatta ctaccggagc cgcaagaaag
     5401 gaaaaaaggc gaagcctaac tgagggtttt gttgatgtag aaaccagccc cgctcatcgt
     5461 caggataaag atcacgatcg ccagcaggta ggcaatcccc tgttcgagga acaaagggag
     5521 cacaaggaga aatacaagca agacgatgaa actgaccacg gcagagagga tatcgagtac
     5581 ccgtgcatcc atgcccataa gttacttcgt tcttgcagat atcattatct tcttcttccg
     5641 ggaactccgt tacccgggga cggtgaagga taggtaaatt accccgggag gtgcaaggga
     5701 taatgatgag cacaccgggc gatgaacggg aggttgtgct gcgcgaactg gaggagagtg
     5761 taagggcact ctcactcggc atggctgtcc tccttatacc tgaagtggga tcgaacatcg
     5821 cctacgccac ccccacagcg cgtgacccct cgcaggtggc ggcggtcgaa gggcgcatcg
     5881 tgcgcctttc agggcgggcg caccccgtgg ggcaggtctc gtttggtgcc agcgatcacg
     5941 tcgcaagaat cgtgctcacc gccatgcggt tcgacccgca ggtgaggtgt gccgcgaaca
     6001 tccgttactc ccccgaaact gtcagaatcc tggaggagat gctcctcgag atatgctcgt
     6061 ttgaccgctc gtcagagcct cccggggtga agaccatgga ctggggggtg gcatcatgct
     6121 gcagggatgg agttcccgat gtgatctacg atcttgggtc cgtgggaaag gagccgatga
     6181 tccgcctgct cggagagaac cctggtgcag tcgtaaaaaa cattcttaaa gtctcggcgc
     6241 gtatgaataa agaataacca agaggaatga ccacatggga atcaaaccca cctacatcaa
     6301 ggcaatcgga attgagctgc tgaaccacca tgggaaccgg ttctccaaca actttgacga
     6361 gaataagcag accatcactg aagtcacgaa tatcgagagc aagagggtga ggaaccgggt
     6421 tgccgggtat atcaccagaa aggtaaatac caaaagacgc tgaatacctc tctcgtatgt
     6481 tcgagggagt tattccggct attgtcactc cttttctccg aaattccaca cgggatgtgg
     6541 atatcgaagg tctgcggtcg aatatcgagt tcctccttgc gcacggtgtg cacggtatcg
     6601 tgccctgtgg ctccacgggg gagtcggcga ccctcacgtt tgaagagcac gaaaaggtga
     6661 tagaggtggc catcgatgtg gcggacggaa aggtgcccgt catcgccggc acagggtcca
     6721 acaataccgc ggaggcaatc cgcctcacga aggctgcgcg tgacatgggt gccgatggcg
     6781 cactggtgat cagcccatac tacaacaagc ccaaccggtc ggggctcgtg aagcactaca
     6841 ccgcgcttgc ggaccttgat atcccggtga tcatgtacaa cgtgcccggg cggacagggc
     6901 agaaccttgc tcccgatctc gtgtcagagc ttgcctgcca ccccaacatc gtcgccataa
     6961 aggaggccag tggggacatc ggccagattt cgaggatcat tgaggggacg ctggatgaag
     7021 acttccaggt gatctcaggg gatgacaaca tcacccttcc gatcctcgca cttggcgggg
     7081 ccggcgtcat ctcagttgcg gcaaacgttg agcccgcacg tatggtggcg atgtttgagt
     7141 ccttctgcga cggtgatctc gacgctgcgc tggacatgca cttcacgctt tcgcctctct
     7201 tcagggccat gttcatcgat accaatccca tccccgtgaa gaaggcagtg gagctcaggg
     7261 gcatggcagg aggtccaccg aggcttccac tggacgagct ggatgatgag aagacggtaa
     7321 aactcgcgga ggtcctctcc acctatgact aacgtcgtgg tgtgcggtgc cctgggcagg
     7381 atggggacaa caataggcag gctggtaaac gaggcccccg atcttaccct tgtcggcggg
     7441 gtggatgtca gagcaggcga tctgttcggg accagggttg tcgaggcagc ccgcatggat
     7501 gcgctcctca aagaattgaa gccggacgtg gtcattgatt tcactgttgc aaatgcggca
     7561 gtagagaacg tcaggactgc cgcgaagcac ggtgcagccc ttgtggtggg aaccacgggt
     7621 ttctcttccg agcagcgtgc cgagatggcc cgggctgtcg aggggcacgt cccggctgtc
     7681 atctcgagca attacagcat cggagtgaat attttctggc agctcctcag gcaggcggcc
     7741 aggatgcttc ccgattacga catcgaggtg cttgaggcgc atcaccgcta caagaaggat
     7801 gctcccagcg gcaccgcaaa gaccatcctc cagatcctcg aggaggaggt gggcgagcgg
     7861 gagaagctct acgggcgcga gggcatgagg gagcgggggc gcgagatcgg agtccatgtc
     7921 atccggggcg gagacattgt cggcgaccat gcagtcattt ttgccgggaa ttttgagacc
     7981 atccaggtct cgcaccgggc gtatgaccgc tcggtctttg cacagggcgc cctcatggcc
     8041 gcccgctggg tggtcgggaa gaagcccggg atctatggca tgaacgacgt gctggggctg
     8101 gcatgaaacg ggcgcaaata cccaaataga aacctatttc tttttttatt atccagtttt
     8161 atgctggata cgtgcaacca tgacagcagt tgttgacaaa gagaaatgta ccggttgtga
     8221 gacctgcgtt gacgaatgcc cggcagcagc catcagcatg gaggacggaa aggccaaggt
     8281 tgacaaggac ctctgtgtcg attgcggctc ctgcgtggac gtatgcccgt cagaggccat
     8341 tcatctggaa taaaatcaaa cgatttaata actccaggga tacagctatt ttttacgtat
     8401 gatcaatgtc ggagtgcttg gtgccacggg agcggttggt cagcgatttg tccagctcct
     8461 ggctgagcat ccctggttca acctgacagc gctgacagcg tcggaccgga gtgcggggaa
     8521 gcagtatggg gaggttgtga actggcgact cgacgtcccg ttcccgaaga atgtcgcgga
     8581 tatcaggatc cgcacaaccg atgtgaagag ccttaaagac gtggaccttg tattctccgc
     8641 cctgcctgca gaggtcgcgg cgggggttga gaccgcgtgt gcggatgcag ggctcggcgt
     8701 gtgcagcaat gcaagctctc accggatgga tccgctggtt cccctcgtcg tacctgaggt
     8761 gaaccccgat catctcgcac tgatcgacgc ccagcgggac ggggggaggg acgggttcat
     8821 cgtgacgaac cccaattgct ccaccatcgt catgtgcctc tctcttgcac ccctgaggaa
     8881 attctccttc ggggacgtcc gggtggcgac catgcaggcc atatcaggcg caggattcca
     8941 gggggtcccc gcgatgggta tctacgacaa cgtgattccc tatatcgggt ccgaggagca
     9001 gaagatggag accgagactc tcaagatcct tggccacctg gatgggaaag ccgtgaaacc
     9061 tgcatcactg aaggtaagtg cgagctgtca cagggtcccg gtgatagacg gccatacgat
     9121 ggcagtctgg attgacatca acgaaccggt ggagatggtc agggatgcat tcagggaata
     9181 cgtccccccc atttcgggac tcccgaccca gcctgaaaga tccctccttt accttgacga
     9241 ccaggaccgc ccgcagccac ggcttgaccg gaacaggggc aacgggatga cggtcagcgt
     9301 cggaagggtg agggagggta tccgctacgt cacgatgggg cacaacacca tcaggggcgc
     9361 agcgggtgcg tcggtactca atgcagaatt gatccatacc aggaagtatc tctgaaggtg
     9421 cctctcaatg ttaaaagaca acacagtatt cgtggggaac aagccggtca tgaattatgt
     9481 cctggctgta gtgacccagt tcaacaacgg tgcacaggaa gtctcgatca aggcacgcgg
     9541 caaagccata tccagggcgg tggacacagc agagatcgcc ctgaaccgat tcctggaagg
     9601 ggtcggaaag aaggagatca tcacttccac ggagatcatc gacaccgaca gcggcaagac
     9661 caatgtctca agtatcgaga tcgtcctttc ggcaccggga taaaccgtct catgcgagtc
     9721 tgtcaggatg ggtcgtgcga cagtgatacc ccgcggtgta gaatgtcacg gggaatccat
     9781 aaaaatctcc tcctttttga ggggcggagt catgacctgc ccggtccagt gcatgcatga
     9841 aaaaactctt taatcaattt gttttcgatt ctgacttgat atatctgtca gatgggggga
     9901 taggtactac cagaaaagat ataaccggca ggttctaata tactacacga atgagaggcg
     9961 tagccatcgc agttacagtg ttgtgcatcc tgtccatggt gctccctgta gtggcggcaa
    10021 acgaggaaaa atatggctac atcaccatcc agaacgtgga catcaccctg gaaaagggga
    10081 aggccaatat cgatgtccag tactcgctcg acgaacccac gcgcctgatc gtcctactcc
    10141 tcggcaagca ggatctcaag aaccggatcc tcaaggtcct caactacgaa gaggccgagg
    10201 tggtggagat cgagatgaac cgggcgaacc tggtagtcga ggatgtttcc tacaactacg
    10261 ggagaggaat ctactggttc cccgcacacc agttcaatgt cttaattccg accctccgca
    10321 tcaccacccc ccagatatcc cgtgaattct gttctaccaa gatggtcccg aatggcatcg
    10381 ggtattttga taatcctgaa tgattggagg ccggaggcct cttatcctga atgattggag
    10441 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgataatc ctgaatgatt
    10561 ggaggccgga ggcctcttat cctgaatgat tggaggctgg ggggcatttt attcatcccc
    10621 gctgccagaa aattaaaaaa ccgggtgttt ccttgatttt ccggcgacat gcatgaccgg
    10681 ataccttgtg atctcaatgc aggacgacag gatttatagg aaaggtgaga gatccgaggg
    10741 acacctttca ggggagcctc cgtcattgag agggtggaag ggggagggac aggctgtccc
    10801 ggagagagag acctgggggc cgcaatgttt aggagacgag ctctacagac tctccgcttc
    10861 gctgtgaagc aaggtatttg ttcaccatgt actggatccc aatacctgaa agggcgcaga
    10921 tcaggccgcc cagggtgcca tagaatatga actggaccgc agtgctggtg ctgtacggga
    10981 aatcggggat gttgttcatc gagatggcat aaatgcaggc cccgtacgtg atcagcccaa
    11041 ccgcgccgat gaaaaagggg aagacaatca cctttgcaag gccgaccctg tcatagaaat
    11101 aattgtcaat gatgatccct atcgaggaga caacgccagc aagtgccagc catagcacgg
    11161 cgccatacat gaatatgagg aagtaaagga ggaggccgat ctccgcataa tagacgagga
    11221 ggttcatcag ccccatgatc acgcccacga ttacgaggag aatccctgcg atatacgtga
    11281 tgaaagagaa cttaccgcgg gtcagagagg tgcgaagtgc gtgtgcaaat ccctcgaata
    11341 tctcgtcgac tccaaaaccc cggtagagga gatagatgcc gatcgcaccg atcacgatga
    11401 aggtggcgat ctgtggcata tcgacaaggt acgcgaccgc ccagagaagc atcgccagtc
    11461 ccagggggac aagcacaagc ctggccacct tcgggtcgtt cagaagcttc ttgatgatat
    11521 aataagtgcc ctcgaggttg ggcatctggc ttaccaccac tctccggatg ctcgaaacgg
    11581 ggagctttga ctggacgatg gggatgacaa actcgtcttc tgcgccatcc gtgaccacga
    11641 tacagttcga ggcccccgtt tgcgccacta cgtccccgag agatgcggca atcctccggt
    11701 caccctcgat catgtgcatg tggttccccg cgatgactgc cacctcgaca tcttctccac
    11761 gtccgagcaa ctcgtcgtat atcttgatgg cctggaagat ggcgttcaca tcggagtctt
    11821 cgggatccgc aagtccaagg gtatttgcgg ccgcaagaca ggcatcacgc ccaatgacag
    11881 ggctatttac acttgcctta aaaccgatat catcgtcacg gtccacggaa aggacaagcg
    11941 tgcgcccctg gtacatcaat atagaaggag caagctatct attaaatgat tgctttacca
    12001 gggtcaaaaa aaggaattaa atgagctttg acctctgcag gagcaggata tcgtctgtcg
    12061 tgagtttctc tcctgcccgg aacatcttga agaggtgctc agcctctttc cgcacggcct
    12121 tctgctcctt ggtgaccttt gccttcttgg tcttcttgcg gagacccgag atcaccttgt
    12181 catagtcgcg gagttccttc tggcaggcaa tgaaatactt gtgttcggca tctgctgact
    12241 cctgcgcttc gacgaaattt ttgtgggcgg cgtcagcctc ctcccttgac ttgtcggctt
    12301 tccggtaaaa atcgaccatc aggtcgtggt gcttctgcgc aagctccgcc atctcggtga
    12361 cctttgcatg caggtcggat gcctgcttcc ggagatccct tgcagaggag atcttggcct
    12421 tgatctcctt gttctgctcg agctccgcct cctgttcccg caccgactcc ttcatctgct
    12481 tgatcttctc gatcaactcg cgttccttgt cggttgagaa gacctcggtc tgctggcgga
    12541 actcgaggtg ctcaatctgc ttctggagct cttttaagcc ccggttgcgc atcccgccat
    12601 gctctttctt gaaggactcg atctcctcga agaaggcgtt tgcctgctcg ttgatctggt
    12661 tgcgctcgtc cttgatggcc tggacgtcat tgttgtattt gtcgcgcagt tccttgttct
    12721 tctgcgcctc ctcaacgtac tccctggtct ggttgttgag agtgttcctc tcccttgccc
    12781 ccttgctcgc aagggcgttg agctcgttcc ttctgttctt gtgctgttca gactcggcaa
    12841 gaatcttctt tcgtttctcg atcagttcat tcaacatgct ctaccatcct cgacggatac
    12901 gtcggaatgc ccgaatccca cgcaagttta ggaaaatgcc ggagaaaaga tggacacaca
    12961 atgcagaaat ttggagatct tgcctctgtc cggagcactc cgtggagaga acgcccacgt
    13021 tcatgcatcc cacggctcca ttgaaaaaag cgccacggca cctgcccggg cctttgttca
    13081 tacggctgtc catcatgttc gactcccgat tccggaaggg gactatcagg ttccctttca
    13141 gccacccttc tctggcaatc ccggatgcat ggaatccggt gattttctat tatttacacg
    13201 atatctatcc tattaattgt ttcgccaggg cggagaaaaa cggccttgga atggtcttca
    13261 cagcgcagga aatcatgaaa aaagtatggg gaacaggaag ccggtattac cggctttcac
    13321 agttctatat tggcagttgt gtgaatcaag gtggtttgtg cagtgctgat ctaacctacc
    13381 cactcgagta aggtacctcc tgccgtctgg tcacgtgcct tcctgttcct cgggagctgt
    13441 ggtgctttcg ggctcacctt cttctgttcg aggtcctcaa ggattgtctt gtaggagacc
    13501 ctgctcccca tgatctttcc tgtctggacc ccggtcatga tggcgagtac cctgaccttg
    13561 ccctccatgt cgttcttcac ccgggcgccc cagatgacat ctgcgtgggg gtcgagctcg
    13621 taggtgaggg aagaggcgat ctcctctgcg tcctgcaggg tgaggtcgcc gcccccggtg
    13681 atgtggatga gacttcccgt ggcaccgcga tagtcaatgt cgagcatcgg gttgctcagg
    13741 cactcgcgga ccacgctctc ggacttgttc tgctgtttgc tctccccgac cagcatcacc
    13801 gccacacctc ccttgctcat gattgccctg acatcagcgt agtcaatgtt gatcagcgag
    13861 ggctgggtga tcgtctcgct gatccctttc acggtctcac cgatgagctg gtccatcact
    13921 gagaacgcct gtccgagcgg gaggttgggt acaaaatttt tcagcctgtt gttatcgaga
    13981 acaataaccg aatccgcggc ggaggcaagt gcttcaagcc cctcttctgc gcggatcagc
    14041 ctggctttct cgacctggaa cgggtagctg accatcccca cgacgatcgc accctgctct
    14101 tttgctatct gtgcaactac gggggcagac ccggttccgg tcccgccgcc catccctgca
    14161 gtgatgaaac agaggtcggc attctccagg agtgcctcga gcgtgggccg ggccatctcc
    14221 gcagccctct ttcccacgtc gggatatccg ccggcaccaa ggccctttgt gagggatttc
    14281 ccgatgagga ctctcttgtc ggactggatc atgtccaggt gctgtttgtc ggtgttgatg
    14341 gcgatggtct ctgcgcccga gacacccata tggtgcagcc tgttgatggt gttgtttccg
    14401 gcgcctccac acccgatgat tacgatacgt ggttgcccta tgaactcgtc atcgattgaa
    14461 ctcgaggtat ttgccaactc tctttcaatt tcgaagttct tctgggcatc gttgatgata
    14521 ctctgcattg ttgaccctcc taaaccttga atgaaacctg gtcactctct ttgaggatac
    14581 gcccgccgcg tcgttctatc aactggcgta ctgcatcccg gactgcttct gagacagtgg
    14641 ggaactcccc tgccgccacc atctgctcaa gcatctctat ctgctggtct ggcagtctga
    14701 gggtgatgcg tttcattttt cctcctgata tgacaattgt ccgacaattg tctgacattt
    14761 gtctgtcaat tagcttgcta atgtctgact atgcagatga aacctacttt atcaaaatag
    14821 tatataaatt tttctattta agtttacaca ggtgatgctg cgttcccatt catttatcat
    14881 actcctggcc acaaatgtac gttcgcatct ccgggttttt aaatgaatgg gcagcatctc
    14941 tcaaaagtct tccatggtgg gaccggtatc cgggcaggca aggaggcggg cgcaccgctc
    15001 ctcgattttt cggcaagcat caacccatac ccgccgcacc tctctttcca gctcgacccc
    15061 tttcgcctcg aacattatcc tgatgacaca tacacggaac tcaaaggggt cattgccggt
    15121 cgtttttgcc gggacgtcga cgagatagca attggtaacg gctcgataga gatcatacgc
    15181 gtcctctgtt catcggtcct ctcaccaggg gacagcgtgg agatctcgcc gcccactttc
    15241 ggagaatacg aattttctgc acgactcgca ggtgcctcca tcggaagggg atctggcaca
    15301 cggctccggt tcctgtgcaa tcccaacaat cccaccgggc atctcgttga gagagatgaa
    15361 atactttcga tgctcgaccg gtgcgaagag gatgaggcac tcctggcggt ggacgaggca
    15421 ttcatcgaga tctcggatga cccaatgcag actcttgtcg gaatacggca tccctcactc
    15481 ttcgtgatgc gatctctcac aaagagcttt gcagtgccgg gcattcgttt tggatatggg
    15541 ttcggcaacc cggacctcgt cgggatgctg gaagcccgcc ggttgccatg gagcgtcaat
    15601 gtcgtggcag agcatctagc gatagaggca tttttgcggt atgaagagct gtccatctcg
    15661 cgtgaacgca tcaggagcga acgtgaacgc ctctgcaggg gcctcttgga tcttcctctc
    15721 gagattgttc cttcccggac aaacttcctc ctcctcaata cgaaaggcga tgtagttccc
    15781 cttgttgaat gcctgatgcg tcgcgggata cttgtcaggg attgccactc atttggcctc
    15841 ccgaattcta tccgtgttgc tgtgcgcacg agggaagaga atctccgcct cctggaggcg
    15901 ctgtcggaat gcttgcgctg attcttgccg ggggtattgg gagcaggctt gctctcggtg
    15961 agaaacctct tgtcaccatc gacggcatgc cgatgatcat gtacgtgatc gaggcattcg
    16021 agtccgcagg attcgatgtg gtggcggtcc tctccaggaa gacgccctat acccacaact
    16081 ggtgccgtgc ccatgggatt tctcattaca ccgcggaggg gatgggatac gtggaggaca
    16141 ttgcagagtg tgcagatgcc catgggataa ctgggccgtt cttcacttcc gttgcggaca
    16201 taccctgtct tgagagagac atcgtgcaga ctctccacga agtctaccgg cacagtcaga
    16261 aggatgcgct ctcaagctgg atcccacggg gccttgcaga aaggtttggc tcacgtgtcg
    16321 cgtacattga aaaaatcaat ggtctggatg cctgcccggc aggcatcaat atcctccgcg
    16381 gggattgcat ggggacaccc caggacgaga cccgggtcat tctggacgat ttccgccttg
    16441 catataatat caatacccgc gaggcgcttg atctcgtcac cagggtgatc cgggccagaa
    16501 ggaacctggg caggaattca tgagaccaat gatctaataa acacgggttc aagcctgttt
    16561 tttatttttc aggaaaatca gggttttttt cttgagaagt aatttaaatc atcgtggcag
    16621 aaataactgt atatgcaggt ctcgcgggaa gtggaactcg aaggccatat cattgactct
    16681 ggtatcatga cccgggtctt tgataaaatt atggatatgg gtggaaattt tgagattatc
    16741 gtttttaatg tcggaaaacg aaaatctgac ccgagttatg cgcacctgca ggtaaatgca
    16801 gacaccgaga accagctcca tgctatcctc tccgagatac atcgctttgg agtgcgtctc
    16861 ctcgagatcc gcgatgtcac tatcgttccc gccgaaggga accggatagt gccccaggga
    16921 ttttactcaa ctacccacca tccgaccaca ataaagttcg aggggaactg gataccggtc
    16981 caggacatcg agatggactg cctggtggtt gtcgataaaa gaaccatgag cgccgcctgc
    17041 acgcctattt caaagctgaa gaaaggggac cttgtggtgg ttggagagga aggcgtgcgg
    17101 gtcaatcctc ctgaacggcc ccgccatatc tcaaccttcg agttcatgca gggcacggtc
    17161 tctcctgagc gcccaagcga gacgatcatt gcgcggatcg cgacagagat catcgagctc
    17221 aagaagaagg gaggaaagat tgccgtggtt ggtggtccgg caatcattca caccggggca
    17281 gcgctcgcac ttgcctccat catcaggaag ggttacgttg acgtgctctt tgcaggaaat
    17341 gccctcgcga cccacgatat cgagtacaac ctcttcggta cctcgctcgg catggacctc
    17401 tcaaccggga aaccggtgac cggtggccac aagaaccact tctacgccat atcagaggtc
    17461 atgagggcgg gatccataaa gagcgcggtc gggaagggtg taatcactgg agggataatg
    17521 tacgagtgcg tccagcggga cgttccttac gtgctcgcgg ggtccatccg ggatgacggc
    17581 ccccttcctg acgtcattac agactcgatg gaggcacagg atcgcatgcg ggaacaggtg
    17641 agagggtgca acatggtcct gatgattgcc accctccttc actctgttgc ggtgggaaac
    17701 ctcctcccct cgtacgtgaa gactatctgc gtggacatca atccttcatc ggtgaccaag
    17761 ttgatggaca ggggaacgct ccaggccatc ggagtggtca gtgatgcagg cacgttcctt
    17821 cccctgcttg agaaacagct tgaattgcag agcggctccg cctagcgtgt cagcggcatg
    17881 cagtaggggc gctcctcctc cagaacaaac ttccattctt ccttgcaggg acaatcggtg
    17941 gagaggagag cagagagaag cttcctgtcc gcagagagcg aatagtccct gatacccttg
    18001 acgagagtgc ggtcgcatgt cccgcagttg tggggccccc tctcctgccc tccgcccagc
    18061 gggtcacatg aaaggtgtac gggagattct gcgaggatgg agaggatgct ccacagatag
    18121 ggagtacggt atgccccctg cttccagtaa tactcaagct cggtattcct ctgcacggta
    18181 catgggttga gagagatcat gtctgcaagg ccgtcgagat catggatcga ggagatcata
    18241 tcgtcgagag cctcgcgttc ggtcaggaaa gggggctttg caaggagata ggccttcacc
    18301 ccggcgcccg acgagcgggc tcttgctgca gctttcacga aatcgtccca ggagaaaccc
    18361 ttgttgatgc acttctctcg caccatgtca ttgctggtct cgacacccac tgcgacatac
    18421 agtggggtat catgggctcc gctgtcgatt gcactcagaa atgcggagag gtggtcatcg
    18481 gacacgtact ccggccgggt ctccgcaatg actatctttc cccggaccat ctctgcaaga
    18541 gcgtcccttg cccgcacggg cacttcactc gtatcaaaga gactgccgga tgtgtagatc
    18601 ttcactgcct caatctcatc gaggcaaaaa ttgttcctga tccaggatac ctgggacaac
    18661 agccgttctt ctagacctcc ttgtcccagt tctccatatc tttcatggcg gtagctgcac
    18721 atgcggcacc ggttccaggc gcacccaccg gttttgagga tgatagtgag ggcagacatc
    18781 acctggtccc ggatgcggtc gctcccggtc catgccgcaa gcgggcgttc aacgggtata
    18841 ttcatggaca cttgctgatt attagagccc ctggctctta agaccctgta tcaacgcaag
    18901 gaggatgctc tggaagggtc cagccagtgc cggtttccta atcgccggtt ggaaaaggat
    18961 ctttcgcgtg tttccgtcga aggcggcaat tctcttttac gaatgatgcc ggggcagttt
    19021 ccccacatga gttgttcctt tctcccaggt attaaggcgc gatgcgtgcc aaaggctcat
    19081 ttcgtctttc tattgcggtt ctcacgaaaa ttcaggatac aacggggtgg gaagaccaga
    19141 agactattca atgctgaacc ggtatattta cagtactaaa tgtgcatgga atggttcaat
    19201 gtggcattcc agggcatata tcggggtgtt tttcccctgc aaagaagagg ccaggtgcaa
    19261 tagaatgaca cgtatccgac agattatccg ggcaaagttc cgggtggtaa ttctcatgtc
    19321 agtattcctg ctattgcttg caggagtgtc tgccgcgtat gagctgtcaa tctacgcccc
    19381 gatgaatgtg cagaggggca tgccgttggt ggtcaacggg accagtaatc tgccggcggg
    19441 gatatctgta gacgtgatac tctcccgctc ggaatatacc acagaggaaa ttgccaggga
    19501 aacggtgacc ctccagggcc caaaagagtt caacgtggtg tttgacaccc aggaactctc
    19561 gaaaggccag tacaaagtcg aggtaccggg agtccaggga tatgcgtttc tcggcgattc
    19621 gaccacgatc agggtggtgc agatcatcga tcgtacggac gaactgacca taaaatcccc
    19681 ccgcacccag gagatggacg ggagcctcga attgcgtggg ataatccccc acatgagaaa
    19741 ttcgggaatc caggtccagg tgatcggccc ggagaacgag gtggtatttg gccccgagta
    19801 tatcgcgacc cagtatgacg gctcattctc aaaggagatc aggatagatg agcccgggac
    19861 atatgaggca agctttaccg acacgaaggg ttacatcggt acatatccat tcgaagtcac
    19921 gccaaaattc gagccgacac ctatacccac tacgcctgaa ccgcgtatgg ttgccgtcac
    19981 tgccaccgcc cattcgagta atgacgggcc agcctatttc gtagtcaccg gggggtccgg
    20041 aaaagcgaaa atctccacgt ctgcgggaat cgactgggtc gtcgagtata ccgatgcccg
    20101 gggtgccatt cagaagatca acaacaaggg ccagatcgat cccgaagaat ttaccattga
    20161 ttcaggaggg gagaccatct acctgaaggt gtacccgtac cggttctcgg acgaaggcga
    20221 ggtgaccatc tttgccgagg gtgcggacag ggtgggtgtc gcgacggagg ttccccccgc
    20281 gtttgcagca accgccacac caaccccgaa ggctcctctg ccgggccttc tcgccctgtt
    20341 tgcggtgctg gcgctgggcc tgaggcgtgg attccgctaa tccctcccgc atccattatt
    20401 tctttgaagt agccggatac atttttgaga atcaagcccc aatactgtta ttcgggccgg
    20461 ggtagtctag tccggaaagg cggtggcctt gaaagccact ggtgcccagc acctcaagag
    20521 ttcaaatctc ttccccggcg tgataacgcc aatttcaact cttttcagaa aaataagctc
    20581 taaaatatct ccgattatta cccaatagga atcgacagat caatatgtaa tgatagcgcg
    20641 ttccagataa aaaaaatata ttcaatgatg aaataattcc gttgcgaaaa attcattccc
    20701 ccgtcgtttt actccaattc ctgcaaatcg atgatgcggg tcgaggatat tttccctatg
    20761 cccggtgtgg ctacgcatcc attgttggtg tagagcccga gcaatgtcgg tatctgtccg
    20821 gatttcatct ttgaacccta tcaccctccc ttttggaatc atcccgcaat tttcatagga
    20881 gccggtgaga gggccgcaat cgccatggaa tatgctccct ctatgtgcca tagtcccgct
    20941 atgcttttcg gcacaatgaa tgagttcctt gctaggaaga acgggatgaa gacctcgacg
    21001 gttgcgggtg ctatttacat aattgagaat ccgactttca atagaagagc atgaatttct
    21061 taccattgtt attcctatta ccaagaatcg gcagtaatct tttataatct ggacttaaaa
    21121 ttaagcctcc tcgctgtttt gtgtggggag actgaatcga ggttgttatc tgcatatcaa
    21181 ttcagtcact ccaaccgccg cgggggcgcc ccgtcgtggg cggcggcgtg catcgcatat
    21241 gggggtgtgg gggcggacag cccccacaat atgtgtccgg ttcaccaatc ctacccacat
    21301 cagatagcaa agagtttttt tgatggaatg ccgtgcactt atgaattcgc acattgttgc
    21361 agaggcgctg tgcactgcat acctgcagag ttgatcggca aaaatataca ataaggggaa
    21421 ttccaggatt gtctgtcggg gggggtgcac tctggacacc ccctgacccg gaaaaaaggg
    21481 ggttttacac catgaacagg acagttctgc atattgcgga aaaagctggc tattatgcca
    21541 ggatgggaat ggagactgca acaacgggaa attataccaa tgcactggaa tactatgaca
    21601 gggccctcaa ggagatgccc ggctacgcag cgatatggcg ggaaaagggt aactgcctcg
    21661 attccatggg caggtgcgat gaggcagtgc gatgcttcga tcaggccctg cagctggacc
    21721 ctggcgatgc agaagcatgg tttgacaagg gattgacact gaagaaactc gggaacgagg
    21781 aagaggcatt caggtgcatg agccggggcg ttgatctgga gatcggtgtc tgaagggatt
    21841 cctcggtcca gtgggggagg gatgatatat ctgactctcc cggataatta ttttctctgc
    21901 attcgacaat tttttttccg aggataattc catataccgg tttcgcagtt caaatgttac
    21961 tcctttcaca ttatccgcca ggatattaca acccattgtt ccgattattg gattgaactg
    22021 gaagccccat tgggcccata tccaattgtt ttaaatgagc caaacagatc acctatgcgt
    22081 gttctatgaa cagcgaactc acacagacac ttcgagaatg gtgcaaatgc cgcgggatac
    22141 cggtgatggg ggtggcgaac gcatccaggt gggagaaacc gccgttttcc ccatggatgc
    22201 atgaatcgtt ctatcctcga tccatatacc cggatgcccg gtcggtaatc gtgatcggcc
    22261 tgcccgtgca cctgccggtc ctcgagacaa ccccctccat ttactaccac gaactctacc
    22321 agacattgaa ccgcctcctg gacgagcata cctaccagat ggcggaatgg ctgaaccagg
    22381 aggggtatcc atccctcttt gtcccgagag acggctacgg cagccttgag gtgttgctga
    22441 ataaacctgt cgcattcttc tcgcaccgcc atgccgcata tctcgcgggg cttggcacgt
    22501 tcggggtgaa taacatgatc cttacacctg gatatggccc gagaatacgg tttggctctg
    22561 tttttacagc agctgagctg ccgcctgacc cgatcatgga ggaggatctc tgcatccatt
    22621 gcatgcgctg tgtcgagatg tgcccctcgc gggccttgtc aggggaaggg tatccagagg
    22681 gtatcactga caagaaggcg tgtgcagagt acagtgccgg gctgtttcgc agatcgatct
    22741 caccatgcgg gatctgtatc aaggtctgcc cggtgggagc cgaccgtgca gcatttcaca
    22801 gggaggacgc ggatatttat caaaaaggtc atacacccgc agggtttgcc agtgcgtggg
    22861 atcatgtcag ggcatatgga gggaaatgac cgggcccttg gggaggttac ccaagagtac
    22921 catttatgcc cttgaaattc caataatcca tcaatatttt gggtgaaggt catgtgtatc
    22981 gctgttccgg ctgaagtggt ggagataaaa gatgggaata tcggcgtcgt tgattacggc
    23041 gatttaaaac aggaagtccg gctcgatctc gtcgatgtga aagtcggaga gtttgtcctg
    23101 gtgcacgtgg gatttgcaat acagaagctc tcccgcgagg agggactcga gacccgcgag
    23161 atcttcaggc aggtgtatgc cgcgatgcag gaatcggaat aaccatctca ttcttttttt
    23221 ggaattgagt attctttttg gtgttacaag acctcattgc accgcctcac aggcctcaca
    23281 gtctttccgg gaatcaatac gcttatcttc taggagatgt aaaaaattgt tgaaaaagag
    23341 gagaggacgt agtcaaaatg gataaaacag gtgttgcgag tcttattgcg ggaatcatcc
    23401 ttgtaattct tggtggatat ggggtatgga tctttctccc cgacgtgatt gtgtttctca
    23461 agggtgtgat cggcatcctt gcgatcctta tcggtatatt cctgttgata ttcggggcca
    23521 tcctggtaaa agactaaaaa gaggttcgca cacggacacg ttcgatttcc gggagcgttc
    23581 acgcttccgg caatctccct ttctggtaag atatcaatct ataaggccta acggctccta
    23641 aattagtgcg ttggggcgca gtatcggcgg ccttctcacc atgaggtgcg actgtggata
    23701 aagtgaaagt tctcatagtg cttctggcca tctccctttg cggaaacgta tttctcataa
    23761 tgagtgccag cgagaaccct gtaaccggtg cagtcagcag gtccatgatg catgcctatc
    23821 accaggcctt tcctccactt gataactcct cggcgagttt gaattccaca gcgaatgcga
    23881 catatgagat gaacgatacg gcggtttatg aaaacccggg ggatgagaac ctctccctcc
    23941 gtgacatcct tatcagtatg gatgacgagg ggacactgga cgaagagctt ctcctcacgc
    24001 tccttgcgga gagggagtgg aacatgagtg ttggggagga ggagatttgg gtggaacccg
    24061 aaccgactcc cgatcctaat gcatggaagg agtattcgaa tgcgaagtgg cgtttctccc
    24121 tcatataccc ccccacatgg gaggtcaagg aaggaagttc gtcaagcccc gcggcgacat
    24181 tcagcgcacc cgttgaggcc gaatgcagtt ccaagaccac cgaatgctac cagtatgttg
    24241 catccctgac cgtttcgatc gaccagaagc ctgagactat cgtccttgag gattatttca
    24301 acaagaaggt gtcgaagctc cagaaagatc tcgcgatcac tgcgacaagc aagagtgctc
    24361 ccacaactct ctccggcaac aaggcttact ggatcgagta ttacacccgg gactccaggg
    24421 gtaatccatc aaagagctac atgcagtatt tcacgatcct tgacaagaaa gtgtatatcc
    24481 tgacctatag cggccccttc tcgactgccg agaatgtcta cagcaggaat aagccagaag
    24541 tcgagaagat gataaaagag ttcgttgtcg agagggaata caaaaccctc tgaaacgcgt
    24601 tttccaattt ttcaagattg tccccttttg tgtactcaaa tcccttcgga caagtacagg
    24661 ttgcgttcat tattgccccc aaggcgtata gtagtcctgc atcagacccc tcaatcggga
    24721 ctcgcctgga ttgggcagga gccgggtcct attgcgggat gacaccatgt gcacctgcaa
    24781 ggggaggttg gactttgatc cgcatcaatg aaagaatgcc ggattttgcc agcagaaatg
    24841 ggttaatttt tcaatatttc tcgatatctt cggctcgtat ggtctttctt ccgtcggatg
    24901 ctgccgagtg tgcagcctcc ctcgcgatag cggcgatata ctcctcggtg ctgaccacga
    24961 tagccctcgt ccctcccatg ctgatccgca ctgcaccact gcgctttgca atccggatca
    25021 caggggcgag gggaaggtcg aattggctgg tgtgtgcggc ttttgccttc ttctttgcag
    25081 gcgctttttt cactggtttt ggcatatttg gtgcctcatt gagggattga aaaggaagta
    25141 tttaaatatt gttgatgaag gaggttcaaa agcccatgtt acgttcactt ctgtgacaat
    25201 tgggcaagcg ttgcctcctt ttcctacacg gatctgtcaa cttcataccc agatggcgac
    25261 gatgaggtgt gcattcaatt ttatccggca atagaaaaaa gggaagtgtg tagcagtgca
    25321 acagatgcag tgacagaaaa atatgcgagg gccattttct cttacatgcc ggttttcgtg
    25381 ttcattcccc aatctggcag tggaatggta attattgcat cattttcaac tcaagcccac
    25441 caccgcatgg tgcaggatgt ccacggtggt ccgcagcgct ctccctgcgt ccctgaggtg
    25501 gtgatatacc tcccttttcc tgatggcatc catcggattg tcggtgcgaa ggagagatgc
    25561 catgctctgg atgtagatat catctatcat ccgcatggca ctccttgctg aacggatgag
    25621 ctcctccacc ctctttttat ccctgcccat cgcccgaaca cctgccgctg tgcactttgt
    25681 tccctggaga agcgcctggg ccattcccag gattgcatcg tcagggtgca cttcaaatgc
    25741 gtagatctcc cgtgcggtct cgtaggaata gttcacgata tagtccatct ggcgggagag
    25801 gctgtagatg tcctgccggt cgaacggagt ggagaatgat gccatgagtt tgtcctccat
    25861 atcgtagcgc atggcatcca gctgcatctc cagctgcatg agttgttgcg ggtcatctgt
    25921 cggtgtcttt ttcagccact ggacaagtga gtatacgcat tcgagagtct tttctgcctg
    25981 ttcaacgagc attgcctcga agtcatgctc tatggggaag ataaattcga aaaccccttt
    26041 tttccctttg tttctaccac ttttctcttt ggtcatgata tactcccggt gagagggaag
    26101 aggacgagag agatcccgaa tgccaggacc atggtggcag ggatggtcac cagcatcacc
    26161 agcactatct ctcttccgac agaccattgc acctttcgga tattctctgc agccccaacc
    26221 ccgatcaccg atgttgagat gatgtggctt gaggagacag gagcccctgc gagggtggat
    26281 gcaaccagca cagagcccga tgatacctga gaatcgaatg aatggatggg ttcaatgcgg
    26341 aatatcttcc tcccgagcgt ggtcatgatc cgccagcctc cgctgagtgt ccctgcgcca
    26401 aggagggctg cacatcccaa acgggcccac atagggatat cgagggaagg ggagagacct
    26461 gcggagagga gtaccagtga aatgatcccg agttgtttct gggagtcgtt tgccccgttg
    26521 gagaacgcca ttactgccgc ggccatccag ttgagcctga cgatatcccg gattaccata
    26581 cgctttgcat ttctcagcag gaccctggtt gccttccgca tgaggtatcc tgccatgaat
    26641 ccgagtaata cggacactac caggaacaca acgaccttgg ttaggccggt catctcgtgt
    26701 ggaggtgcca ggaactccag gaatccccag tgcacaccgc caaggccgcc tgcagcaatc
    26761 cccgcaccca ccagcccgcc gatgagccca tgggtggatg atgaggggag tcccgtccac
    26821 caggtcccga ggttccaaac gagggcagcc agcagtcccg caagtacgac aggcaccatg
    26881 tgcgtatctg cctcaagggc aaggaggcca gatatggtga atgccaccgc gctccccccg
    26941 aagagggctc ccatgaagtc cattgcggca acaaagatga ttccctggcg gggtgtggcc
    27001 gaacgggaag agatgaacgt ggcggcgaca ctgctggcat cctggaaacc attcgtgaaa
    27061 gtaaacagaa aagccaggag tatggcgagc caggcgaaac caatcatcga tagtgttccc
    27121 actgcaatca ggttctggct ctcatttatt tgaggcttgc cttctggaca agaggtaatt
    27181 atgacaggta atacgacccg ggcatgttaa gaagatgtcc ccccaaggca cgtgggttcc
    27241 tgaatcaaag gccgggtctt ctcttcaggg ggattcagag gcaagcaata atgactccct
    27301 tcacaaaaag taggaaatga gagcgatctc acatcatgac ccacaaggta ttcgcgaagg
    27361 cgccaacggc gatgaagttg atcctcctcc tcggcgtagt gagcctcctt ggagatatca
    27421 tttacgaggg gggccggagt gtaaccggcc cgtatctcct gtttcttggg gcttcagctt
    27481 tcaccgtggc ttgggtatct ggcctcggag agttcattgg atacgccata cgtcttgtat
    27541 cagggtattt cgcggaccgc acgcgagaat actggacatt ctctcttgct ggatacctta
    27601 tgatcggggc cattccgctg ctggtctata cccgttcatg ggaggctgca gtggtgctgc
    27661 tcctgatcga acgcctgggg aagggtatac gttcgcctgc aaaagacaca atcctctcgc
    27721 acgcagctca ccaggtaggg agagggtggg gttttggcat ccacgaggca atggaccaga
    27781 tcggggcggt cgccggacct ctcgtcttcg cagcctccct tttgctgttg ggtggatatg
    27841 gcgagggatt tgcactcctt accattcctt ttgttctcct cattgcagca ctggtgcttg
    27901 cctggaggcg ttatcccgac ccggcctcga tggaggaaag cgccctctct tttggataca
    27961 ccccaaagga cccccgggtc ctcctcccct atggtgcgtt cacggtgctc acgatggcag
    28021 ggttcctggt cttcccgctg atggcatatc acttcagtgc cgcagccgtt gtccctgacc
    28081 cacagatccc gatctttttt gccgttgcga tggctactga tgcgctcgct gccctcctga
    28141 tgggaaggct ctatgaccgt tggggactcc cggtgataat cgccatccca atcgcaaaca
    28201 tccctatcgc accacttgcc ttctcttcgg gctactttgg cgcccttgcc gccgcagtcc
    28261 tgtggggtgt ctcgatgggg ggccaggaga cggtcctgcg ggcggcgctt gccgatttca
    28321 cctcaatcgg aaagcggggt tttgcgtacg ggatcttcaa caccctttac ggggcctcgt
    28381 ggtttgccgg gagtctcgtc ttcggcctgc tctactatgt tggtgcggga gcgatatgtg
    28441 cgttttccat attcatgcag gtcgcggcga tgggtgcgtt cttcctgctc tggcggtgcg
    28501 caggaagaag tagtcgtggg tattctccat gacgcaaagg agggttttat tcaggggatt
    28561 catggtccag ccaggcgggg aacgttattc ttaaagatcc ccttgcatgc gctggtgcga
    28621 agattattct cctgttcctg atgacaccta ccccgggatg ccttttctgg tccagtgcgg
    28681 aaataatgca gagggagttc gcttttcgcc ttatctctct tccttttccc gccgttcttt
    28741 tatcagtctt gccaccaggt ttacgatctc ctccaggcgg tagggtttct ggatcacccc
    28801 ggaaaacccg tatgatcggt attctgccat gacggggtcc gtggagtagc cacttgagac
    28861 tatccctatc acgcccggtt caagatctct catctggcgg aatgctccca ggccccccat
    28921 gccaccggga atggtgagat ccattatgac caggtcgtaa ggcgtcccac cctccatgca
    28981 gtccctgaac ctggcgatgg cctcccttcc ttctgcaact tcatccacct gatatccctg
    29041 ccgggtgagg atggtgtgga ggatctcccg aattcctgcc tcatcgtcca ggatgaggat
    29101 ccgaccgctt ccgctcggga gaattttttc ctgcgaagat tcagccggct tcacactggt
    29161 ggcagccggg aggtagacag tgaagcatgc accctctcca tgcctgctgt ggagatcgat
    29221 gatcccgcca tgctttttca cgattgaaag ggctgcggaa agaccaaaac ctgtcccttc
    29281 agccttcgta gagaagtagg gatcaaagat ctgcgactgg atatcttcgg gtactccctg
    29341 tccctggtcg gtgacagaaa tggcgacgta gtggcctgga gggagtggaa gtgtgctccc
    29401 ctcaccaagc gtcgtattgg agaccgtgac gccgataatt cctccttctg gcattgcctg
    29461 gtctgcattg aggacgaggt tctggattac ctgggcgatc tggtcaggat ctgcctcaac
    29521 gctccacgca tcgggaggga tggagaaagt gggttttgaa cgggaacctg tgagcatgat
    29581 ctccgttgtc tccctcacca ggggaccgat cgccaccacc ctcttgaccg gcgcaccccc
    29641 acgtgagaac gtgagcagtc tctgggtcag tgacctcgcc ctgtatattg cttcttccgc
    29701 cttcgacagg ttggtataga ggggtgtatc ccctggcgtt tccaatttcg cgagcgagat
    29761 gttgccgagg ataccggtaa cgatattatt gaaatcatgg gcgattcctc cggcaaggtt
    29821 gccgatggct tccaatttct ggctcctgag ctgttcctgc tcgtattttt gcgtcattgt
    29881 ggtgtcccgg aatacgatca ccactcccat gatccgggaa acccggtcgc ggatgggggt
    29941 caccgtcaca gatacgggca gttcccctct gttacgggga agcagtgtga ggtctctctg
    30001 tatctccagg cctccgcact gcccgtgcat ctcagcaatg agatcgggat cggaatcccg
    30061 cccgccgctc acgacaggga acactctgtc gagggtttgt ccttcggcca cctcaacggc
    30121 ccatccagtc atctcctgtg cggcccggtt catgaacctg acacagccct ggaggtcggt
    30181 gctgatcaca ccgtcggtga tggcgttgaa cgtgacagag aggagctctt tctcggcagc
    30241 gaggagttcc tcaaccttga ttcggtccgc aatctcgttt ttcagaccgc tataggcact
    30301 cgcgagctcg caggtcctgg ctgcaacctc ggattccagg gtcttctgat aatcgaggag
    30361 ttctttctcg gcgttcttga gatcggtaaa gtctctgcct acggactggt actccttcaa
    30421 cactccctgt tcatcgtata tggccctgtt ggaccagcgt atccatctgg ggcttccact
    30481 gggcagtatt accatgtcca cgaggatgcc ggaagggttg tcgagggaca gggactggaa
    30541 atgtttcgta agagcatcca tattccctgc cggtatgggt gggtctccgg cgggggcgag
    30601 gatctcctca ctctctctct ggaagaactg gcagaacgca tcgttcgcaa agacaattct
    30661 tccatcaggg gcagtcctga atatcagttc cgtctggtct tccaccaccg cgcggtagcg
    30721 gcgctcactc gaatcgaggg cattgcgtat gcggcgcaac cgtgcattcg aatgcaaaag
    30781 gactgcaatg aggaggacca tgcatgtccc gctgatcatc gcagcccaga ccaggtattc
    30841 ggggatgggg accagcgctg acggttcatt gagaatccgc gatcccgggg gaagggatga
    30901 aacgtcgagg ccgaaccgct gcatctcccg gtaatcgaag acatagtggt tctcaggccc
    30961 gaccactaca gggatctcgg aaggatcggt cccattcatc acctcaagag ccagggaagc
    31021 ggcagcacga ccctgctcat acccgctggt gagcatgcct cccacgatac cgtggccgat
    31081 ataaagttcc cagatggcaa atatagggac tcgggagtgg ggatacaggt attccagggt
    31141 ctcaaggtat gaaaagtcgt ctcctgcccg gtcctggttg aaggtcatca ggaggatgac
    31201 actcccatcg ggaagtgagg agaccttttc ctggagctcc gccatggtgt attcctccag
    31261 gtaggtgaag ttcagcctcc ctgcatacaa cggggcgata tcgctgatca cctttttgtt
    31321 cgcagcgccg gtgagggacg catcgttgat gacgaagacc tcttttgtac caggcagaag
    31381 tgagagggca gcgtcaaggg tgccctttac atcaaaggtc tcgacgactc ccgtaatgcc
    31441 gtctctctcc ccaatcatgt cgggggaata gtagttcact ccgcagaaga tgaccggggt
    31501 gcccgaaaaa agggagtccc ggtgatccat tatgaatttg aacgcattgt catccgagac
    31561 gatgagggca tcgaagcggg tattccggta cttgtgggca tagagggaaa agagggtccg
    31621 gacgtattcg tcatcggtaa agcgcttgga atccatgtat tccacgtgga ggttaatcgg
    31681 agcagacgag ttcaggaaag catcttcaat tgcgtcggtg atgggatctg accactcctg
    31741 tcccctgtgg taggagttga ggtagagtac ctccttcctc tcgggtgaag acaggtcagc
    31801 aggtgagaca gggcagatga gaaagaggat ggcgagaata atgaaataat gggcccatgg
    31861 aatgccgcat ccttccgcaa ataaaaaaaa cgggatcttt ccccttgtgc atgtgtgtac
    31921 tctggccatc aacactgtct cttccccggg ttgcactcat ccatggttgc aggaaagaaa
    31981 agaaaaagat agctgacatg gaatccgaat atcttcctgg catgccgggt gcaagatgcg
    32041 atttaaatca tcccctgctg aatttattca ggctcagtaa tacaaggggg attgtagaag
    32101 cattctgtat ttcagatcag gagaaatatt gtttgacttt cctgtccgga tttttacaaa
    32161 taaaaatgag aataatgcat tctgctcctc gcaccctccg tcaaagtctt cgaacgagat
    32221 accgaaagga agagaatgca aagggcaggc agtgtagcat gtttgcttca ttcgtcaatt
    32281 ttataaaatt gtaatgataa cactaatggg aattttcaaa tccgtggacg gtggaattac
    32341 gcgacccaga ctcaaggatg aactcgaata tgcgatttgg aagattacag gactctccat
    32401 tccgtataac gaacatgtga tacctcatct ctcgcaggag atcgcgagga agaccggtga
    32461 agatcctggg gaggtcagta tgaggcttgc agcacagata aaagagatta tatgggcgga
    32521 tttacagtat cagttccggg acagggctcc ctgtaaaaag gcagtggaac atataacgga
    32581 ttaatactgg ttgaaagtca gcgaccgcct gtctattccc acattcacct gctctttttt
    32641 ttacataaaa ccacaaaaaa aatcctcttt tcctgatggg ggaaataatt tattctcccc
    32701 tcacatgatc tattcgggag aaaaggcatt gtcgtttgag gaatgcgctg tatttgccaa
    32761 ccaacacccg gtctgtgccg tggcgaccgt ggacggggac cagccgcggg tcaggttttt
    32821 tctcatgtgg tacgcggacg ggaccgggtt ttacttccat accggcaaga tgaaacagat
    32881 ctatcgccag ttgcagcaga acccgaaggt cgagctctgc ttctatgatc cgacaccccc
    32941 tgaacagggc ggaggaaaga tgctgagggt caccgggcga gtggagtgga aaagggaaca
    33001 agctctcgtg aaccggctgc ttgccgagag gccatggatt cggtcgcttg gaacagacat
    33061 agaccagttc cttgcaatct tccgggtaaa gggcggggag gcctggttct ggaccatggc
    33121 ggataatctg aaggaggatc ggataccccg ctatgaattc tgaaaaattc ctcctttgcc
    33181 gactgatacg cacaattact gccagctgag agtatagact atgaaagatc gcaacctatg
    33241 catcggaggg ctttttctca tcatcgccgt cgtggtgggg ggaactgtcc tgacctcggg
    33301 gtgcacggaa cagacaagca ccccaaagga gaaccctttt cccgggatat gggtttacca
    33361 tggaaaaatc ggcacctaca acgctaccat tgtgttcacc ttcaatgaga acatgaccgg
    33421 acgttatgac atgctggtgc cggatacgac accgccttat atgaactcca tggagttccc
    33481 ctgggaatct gatgctgacc agctttttat cgggtcaagc agcgagaagc agcaccttga
    33541 cattcggtac tatcctgaca gggataggat agtggtgctc gcggatgaag aatcagggat
    33601 attcattggt gaggagtttg tgaccgggcc ttttggctgg gagttcttcc gcgtgatcga
    33661 gggagaggga acgccaggga ccagttcatg aaggggaggc attctctctg taatcctttt
    33721 acccttgccc tgggctggtt ggagagagag cataacatct gctgatgagg atgattattt
    33781 ggaacccatg acaagattgc agatcctgct ggcgcttgca gttttcaccc tggcatgtgc
    33841 tgggtgcata gatgaagagc tctccccggt ggtcgggacc tgggagtggt cggacaaaaa
    33901 gggatacact gagcgctaca ccttctatgc aaaccattca ttctttgcag aggccctcgg
    33961 cgcgggattc gagggaacgt gggaggagct atctttcggg cattacgagg tgacgtactg
    34021 gaacaggaat gacccgggga gatcagaggt gttccgtgag agggtgttat acgatgcaga
    34081 gacggatcgt gtctatttcc cgggtcacga aagggttgga taaacccggt ggaaatacca
    34141 ttcagggtga agcaatatgt ccctgcccat ttccgaatca ggttcctatg gtccctgcct
    34201 ccagctgcgt gtccacccaa ttctccgccc cgcacttatc ggtcaacctg cagatgagcg
    34261 atccgggtag aactgtccaa ttgattgaat tattgaaaaa aagtgaagag aaggagaatt
    34321 actccttcac ttcctcgatt gttgcagttg cagcattctt cttcacgctt tcgatcccgt
    34381 tcatgcaggc agatttggaa ctgtatgctt ctccgactgc aataatctcg ccgttcgagg
    34441 ctttgagcct gaagcggtac ttgcctgcct tgtccgtgta gacttcaaat tttccggctg
    34501 ccataatact accagcatta cttcatgtat cgggtgcttt taatagtatt gttgggggta
    34561 acattcagaa aatggagaaa acacaggaaa tggagaaaaa tacaaaatta aaagtgctgc
    34621 tatggggatt cgaaccccag tcgcgggcgt gagaggcccg catgattggc cggactacac
    34681 tatagcagca aattgcctga tgagttagga cttcccggca cttataattg ttgatatacc
    34741 atccatgcac cgggaagatt cctgccattt gcatctgcta tcggacccgc acgcatcttt
    34801 catccacgca atcgcgtcgc ttttgggaga gacccacaac tatatgtcca aaatcccgcc
    34861 attcatatag gaacagcaga tggcaggcct ggaagagcag ataaaggagc tcgaggagga
    34921 gcttcagaag acgccctaca acaaggctac tgccaagcat atcggccggg tgaaggcaaa
    34981 gattgcacgg ctgcgggacg aggcggtcac ccgggccatg cgggccggcg ggggaggcga
    35041 gggctactcg gtgaagaagt ccggggatgc cactgtggtg ctcgtgggct ttccctcaac
    35101 gggaaagagc acgctcctga atgcgcttac cggcacgaag agtgaggtgg ctgcttatgc
    35161 cttcaccacc ctcaccgtgg tccccggcgc ccttgagcac aagggtgcac ggatccagat
    35221 tctcgacatt ccgggcctga tcgcgggagc tgccatggga aagggccgcg gcaaagaggt
    35281 gatcggcgtg gtgaggggag ccgacatgat cgtgatcctg gtggatgtct tcaacgcgaa
    35341 ccacgtggac gtcctgatga aggagcttta cgacgctggg attcgaatca accggccacg
    35401 tcccgacatc accatcaaga agacagcact tggcggggtc cggatgaacc atgtcgggat
    35461 tcttgacctt gacgtagagg agatccggtc catcctctct gagaacaaga tgatgaatgc
    35521 cgatgtactg atccgcggga acgccaccca ggacgatgtc atcgatgcga tgcttgccaa
    35581 ccgcgtatac attcccgcgt ttgtcgcggt gaacaaggtc gacctcattg atgatacggc
    35641 tcgaaaagca atcgaagagg agatcctcgc gcggtttggg gatgcgccgc ttctcatctc
    35701 ggcccatacc ggttatcaca tggaggagat gaaggacgag atctacagcc ggctcgggtt
    35761 catgcgggta tacttgaagc cccagagcgg cgctgcagac ctggaggagc ccctcatcat
    35821 caggtccggg agcaccgtcg aagacgtctg ccgcaagctc catcgcgact tcgtggacag
    35881 gttccggtat gccagggtct ggggccattc cgtgaagcac ccagggcagc gggtagggct
    35941 cccccacaga ctcagggaag gtgacctcct cactatcatc atcgagcact aaaaagcctc
    36001 aaaaacgact gtttagccct tgaggtggtg aatgatggcc cctgcaaact cttttgccgc
    36061 agcggggcca tttgcggtga tgatgctgtc gtccaccact acgtctttct ccgagatatg
    36121 cgcccctcct tttattatct ctctttttga tgcagggctc atgtagcagg ttgcagtcct
    36181 ttccttcagg atccctgcct gggccagcac tacaggggag aggcagattg ccgcaaccag
    36241 tttccggttc ttatggaagt acttcaccag attgatcagg atctcattgt cccacaggtg
    36301 tgcaggagag cccgaacccc cgacaatgac aatgcctgca tactctccag gttccacctc
    36361 ttcgagggag aaattcgcca tagcctgcgt gccgagcatc cccttgcacg tccccctccg
    36421 ggtggaagca acctcaaagg agatccctgc gttctgaaac gcagccaatg gttcagccag
    36481 ctcctcgtcg cggaactggt caggtggaat cacgaccagg atcttcatac agggaaaggt
    36541 tctccatcag ggataagaat gtttcccgca gggtggacct gaaaggcggg tgaatgggag
    36601 gaaggtggcc aggtggggca tctcggatat gaagggatca gatgccctga tcatcgatgc
    36661 cgctcctgcc tgcaaggagc acttcccgga tcacatcgag aggcgcatcc gttttcagcg
    36721 cagtcccgct gtagtgggcc ctcgcggcgg catatcctcc tgccacaagc gaccgaatta
    36781 ctccctccat tggtggcggg gacaacctga agtgccgcga gagcacgtgg taatcgtaat
    36841 ggaaagagat gtcgggttcc tgcctgcagg tgttcaccag tcgcagaagc tgcgctgact
    36901 ttgctagtct gaacgcaggg atcgcctctt ccatcttctc taggatctcc ctgtcctgca
    36961 gagacccggt ccagagcggg ccgacgggtg tgagcccggc gccgcactcc gggcaaaacc
    37021 cgggctccgg gagcaatccg gtctcttccg accggaaagg acatgaatgg cactgcatga
    37081 cataccccat ccgggcaacg gttcggtctg cggcttttgc cccccgggag agcctgacgt
    37141 gcacacgcac gaagtgctcg cgcgagaaac aaaagagtgg ctcgataccc cggtcatact
    37201 tcaccgcctc ccggacaagg aatccaagga gaatccgcaa cgccgtttct gcgtgatact
    37261 cgttgttcat ggcctgggca aaatagcgcc ggataccggc atttttgtgg gcaccacaga
    37321 ggggggccgt atcagttgcc gtcacgaata ggtacctgcc tgcccccctg atccccgagt
    37381 ccacaaatgg cgccggggaa ccaaaagggt cgatatcaac cgcttcaaag cacctcccgg
    37441 agaggagcac gttcgcatcc gagtgcagca cctctacggg gagtcccagg agtccggcat
    37501 tccggcgaat gagcgcaaac gccccacggt cgcgatcatt gatggtcaca ggcaccctgc
    37561 attcagaggc gatgcggagc cccctgatcc cggaggccgc catggcatca aggtactgcg
    37621 acggcgaaag gagagagacg aggacaacgg tggcgtcgcg ggagagttcc atccgcgggt
    37681 tgtagaagat cggggcggag gcgggaggaa acgggtggga tggatcctgc acagggataa
    37741 aaaaccgcgt atttccctct tctgcctcga tatacttcat tgccgcaaat gtggcggttc
    37801 gacagcctta taccttctcc ttaataatga atataggcag ggcgtgtggc ctagtcagga
    37861 cagggcggca gcctcctaag ctgtaggtcg ggggttcaaa tccccccacg cccgtttctc
    37921 tctcgttttt ctcctcatta ctaacgatat taatacttcc cgggttgcac ttagagacca
    37981 tatgtgcagg aggaaggagc cattctcccg tatcccccgt acttgagggt gatccgcccg
    38041 ctcaacgcgc ttgttgccgg gtgtgcagcc atcatcggct atttcttggc tgccggcacc
    38101 cttgttccgg gtatcgccgt cctcttcctc gtcgtgttcc tcgttaccgg ggcaggaaat
    38161 gccatcaacg actattttga cgtggagatc gaccgcatca acaggcctga ccgccctatc
    38221 ccatcaggag aggcaagcgc gaagggagtg cttgtcttct ccattcttct tttccttgca
    38281 ggaattgcgc tctcgttcct gacaaacatt ttctgcattg taatcgccgt cgcgaactcg
    38341 cttctcctcg catggtatgc tgctgccctg aagaggacac ctggggcagg gaatgccgcc
    38401 atttcatatc tcactgcgtc gatcttcctg tttggcgggg catatgccgg ccccgacggc
    38461 ctgcagctcg tatctccggt ggccctgatc accttctttg ccatgttcgc acgggagatc
    38521 tggaaggatg cagaggatgt tctgggggat gttgccagtg gtgccgatac tctccctatc
    38581 aggatcggag tgtatcctgc aatctgcctt ggatttgtat tccttgtggg agcgcttctt
    38641 gcaagccttg tgcctgtgct ctggtggggg ccgtattacc tggccgggat cgcctgcgtc
    38701 gatatcctca tcctctgtgt gggtatcaga gccctgggct gtaaaaccgc tgcctgcctg
    38761 aagaaatccc gggttaccac cctcttgaag tatgcgatgt ttgcgtctct ggcggtattc
    38821 gccgtgtccg cattccttct ctgaacatgc ccctgtcaaa gacttcctca cttcaaccat
    38881 cccctgcagg gacgattcag atgtgcagac gggaaaacga gatagagagt tgcggtgaaa
    38941 aaaggatgcc gcgtctgcat atccggttat tctcatcgtg aatgtgtgtg cgggacttca
    39001 ataatcttct gcgaccggcc agctgttcac ttcctttgcc tcgggaggga tcccggccgg
    39061 gggcacgccg agggttgcaa cctccatcac cttgtcccag gcgacgagtg tctccacaca
    39121 cccatccttg agcgtcacca ctcccatggc cacaagcccc atcttgtcgg acatctcgag
    39181 gccttctttc acttcggaga ggcagccgac accaaggatg gcgcgtgggt ggtatttttt
    39241 caccattcgc ttgatgaagg aagagccggg aacgatgaac accctgtagc ccatcgcttc
    39301 aagtatcttt ctccagaaac caatggtgca ctcgccacaa cttctacact tcaggccctc
    39361 tggagagagg tgggccgggc accgcgacga ccgaaggcac tggggaagga agatcgcccg
    39421 ttccgtgacc gggatctcgg agaaactctt cttgttcatg gtgttgtgca ggttgatgaa
    39481 gaacgtcagg acctcgctgt cttcaagccc gaaaaggcgg aagagtgatt tggcaagtcc
    39541 ttcaacgagc accataccgg ccttgagaaa ccggggaaag taaagcctcc cggtattgat
    39601 tgaatagatg gaaagtgcga cgagaatgag tgcaaagaag aagatcccaa taatgacaac
    39661 gattgtgatc tctccgatca ggaatacgag actctgccac atggaagcat caattggcac
    39721 aggcctgcat ccccctaaag gcccctgtcc tttcgggcgg cctcgatctc attccagtcg
    39781 accggcgggc tgataacacc gatatccgta atgaacgccg tgaccagctg atggggagtg
    39841 aggtcaaatg catagttttt cacacctacg ccgtccggga ccgtgacagt gctcccacaa
    39901 aaggcaacct cgttcctgtc tctctgttcg atggtgacct cgcgggcgat gcggtgtggg
    39961 tcgaacgtgg aaagcggcgc ggcaacatag aacggtacgc catggtggct tgcgcagacc
    40021 gcgtgcatat aggtcccgat cttgttgaag acgccgtcag gggtgatcct gtcggcaccc
    40081 agaatgactg cgtctatctc tcccaacttc atgaggaatg ctgcagaaga atctgggatg
    40141 acggtcacgg gtatgccgtc cctctggagt tcccaggcgg tgagccgggc cccctggagg
    40201 agcggacggg tctcgcaggc gatcacctcc acatctgacc ccgcttcaac cgcggagcgc
    40261 accaccccga gcgcagtccc ccactcgctg catgcgagtg ccccggcatt acagtgggtg
    40321 agcaccgtgc agcgctttcc gatgagggcg gctccatagt cgcccagttc atggcaggca
    40381 cgggaatctg cggccgtaaa ctcccgcgca gcacgcagcg ccttttccct ggcctctgtg
    40441 acggagtgcg cactctcgac aacccgcatt acgatttcca cgccaaggga gaggttcact
    40501 gcggttggcc ttgcatcggt gatgcgtgcc gcttctgcct tcagcatctc catgaagcga
    40561 aagtgttctc tctccctgat ctttctgctt gcaagcacca ctccgtaggc ccctgcgaca
    40621 ccaagtgcag gagctccacg gatctcgagc ctcctgatcg cggtgatgag acggtcgacg
    40681 gtccggcacc tgacgatgcg gaagtgtccc ggcaaccgtg tctggtcgat gagagtgaga
    40741 gcgacctcct cgtcatccca atgcagggtc tcaactgctc tcattcggga tatccttccc
    40801 tggtcgcatc gataaaggct cgcatggctg cagatcccgc gtgggcgaca cagtcaggga
    40861 tgtcccgggg ctgggtcgtg gtccctgcca ggtagatccc cggtcttacc gtcgccacgg
    40921 tctctatctt gtcatccagc ggcctgaaaa agcccgttgg ctcccgggga atgtcaaaga
    40981 gggtagcgat ctctcctgca tctgcaggcg gctggagacc tacggagagg accaccagtt
    41041 caggacggag ttccagcatc tccccgttct ccgtgttctc gatcctgata aatagcgagt
    41101 cttcttgttc aaggatctcg gcgggcattc cgcgcacgaa acggacaccg gcatcgattg
    41161 cccgctggta atactcctca tatcccttgc catacgccct gacgtccatg tagagaatcg
    41221 tcacatcggt ttccgggtac ttctcccgaa tcagcatggc attcttgatg gcatacatgc
    41281 agcagacacc agagcagtat ggcctgtcga tctgcatgtc ccttgaaccc acgcattgga
    41341 cgaatacgat cgagcccggt gtctttccat ttgagagcct tcggactttc ccaccggtcg
    41401 gtccgctggc attcatcatt cgctcgaact cgaggctcgt gatcacgtcg ggataacgga
    41461 gatactggaa ttgctccttg tttcccgcat caaatacatc atatccgcag gcaatgatga
    41521 tgcttgccgc attcacggtg attttgcgct ctgtatcctc ttttcgtatg gcttcgcggc
    41581 cacagacgtc gtaacacagg ccgcactcta tgcagtgctc gggatctttg accacgatat
    41641 cggggactat ctgctggtgg ggtttgtaga ttgccttccg gaccccgata cctgcatcga
    41701 agcggttgta tacctcaacc gggcagatat caatgcaatc cccgcacccg ttgcagcgtt
    41761 cttcgtcgat atagcggggc attttggtga tggtgacaga aaattggccg gccttaccag
    41821 agatcgcact caccttcgcg caggtgtggg tatggatgcg agggtgccgc aagacgtcca
    41881 tcatcttcgg ggagaggatg cacatcgagc agtcattcgt ggggaatgtc ttgtcaagca
    41941 tcgccatgtg accgccgatg gagggctctt tctccacgag gtggacttca attccgtggt
    42001 ttgcgaggtc gagagctgcc tggatgccgg tgaccccccc gccaacgatc accacgtcag
    42061 acatgcaggt aactccctgc catctcccag taattcctgg cattctggag gatatgctgt
    42121 ctctgggctt ccgtaacctg cttgatgact tttcccggga cacctaccac cacggatcca
    42181 ggggcgatcg tggtcccttc cgtgaccact gagcctgcac caataaggca gtcctcaccc
    42241 accactgccc cgttgagcac gatggccccc attcccacga ggaccctgtc cgatatagtg
    42301 cacccgtgca ggatggcccc gtggccgatg gacacatctt ctcctatgcg cgcggggaac
    42361 cccttgctgg tatgaatcac tgcattgtcc tgtacgttcg accggtctga tatgacaatc
    42421 ttatccctgt ctccacggac cactgcgccg aaccatatgc tcactcccaa cccaagtttc
    42481 acatccccga gcaccgtggc attttcagca acaaaggtct ctgccgttcg cgtgcgacca
    42541 acagccataa tagcataaaa aaatcaatat gtggcaccat gaaggttatg gtcgggggaa
    42601 cattcgatcc tctgcatgac ggtcataaaa ggttgatctc ccgttctttt gaacttgcgg
    42661 gaaatggcgg cgaggtgacc ataggcctga caactgatat cttcgcatca cgaaagagcc
    42721 accccgttcg cccgttcaac gagaggatgg agtcactgga gcggttcata cgggagaacc
    42781 gctggaatgc agcgtggtcg atcgagccac tgaacgaccg ttttgggtcg gcactcgtcg
    42841 cggagttcga cgccctggtg gtcagtgaag aaactctccc cattgcccgt gacatcaaca
    42901 ggattcgtag ggaaaagggc cgctcgaagg tcgacatcca ccagatcacc tgcgtccttg
    42961 ccgacgatgg aaaatggatc tccagcacga ggatataccg gggggagatc gacgatcacg
    43021 gcaggctgct cacctgaaca tccctcccag ccctcacatg tactttttca ttcgttgaac
    43081 cacagccatc aacagaattt atatggagat ggggacgagg gtgtgagtga tactcacagc
    43141 atctcttttc catgagtgtg gttttcttcg tgctctcctc gcgacaggag ggtctgtctc
    43201 tcgtcctacg ctggaaaaat tctctcttta tagcatttgc gaatgtccag gaatgacagt
    43261 tgaaccgatc atatcatgta cattgataac aggaaacccc taaatttgag atcgatactc
    43321 tcagcatcat tttttctcaa ttttcacatt cctttccaag acgcaccggt aaaaacagat
    43381 taggggatat atctcttctt tgagtaaaaa cctagtgccg ctccggttcc cagggggacc
    43441 aggagccaat ggagaggtgc ctttgcagtg gttgtctggg ggacggggga attctcgagt
    43501 tcccctatga ggaacatcgc catctgcacc gcttcatctg cttcctctgt ccggccaagg
    43561 ttggagagtg cggttgatcg gtcgatccag gcctccgaga gtcgtgggtc gagctcgatg
    43621 gcgcgatcgg cggcctcgag tgcctcttcg ttccgcccga gggagatgag ggcacttgcg
    43681 cggttggtcc aggcctctgc ctggttctcg tcaatggcaa gcgccctgtc cgaagcctcg
    43741 agcgactggt tgtagtctcc catcgcgttc aggatgccag cccttgtgat ccaggcgacg
    43801 gtgaagttcg gatccatgga gagtgccgca tcgatggacc cgagcgctgc ggagaagttc
    43861 ccctccacag caagatcgat accgtggttg tatgcatcga ttgcctccag gggcggtgcc
    43921 gatgttgcac atgagacccc tatacctgat atacacaacg caaccagaag gaaaactcgg
    43981 taattcatgg aaaaatcctg tcctttctat tcggttttat gtatgaacgg gaaatacttt
    44041 ctgttttccc ttcagccgcg atccgcaggt gcatatgctg tcaggctatg gtatggtcca
    44101 tcttccaggg tttgagaatg gcgacccgaa cagcaatttt ttagtatgca ttggaccacc
    44161 gggaaaagac ccgcaccatg cagttcccca aaaaaaccac agggtgatgg gcatccggca
    44221 ggaaaggctc gccagtggtt tgacccccct gttaccgctc tgatcgcata gatcgccggt
    44281 atatgagagt tcggattcat tcaccgtgaa gagccccggc ccggctccat tgtcgtgttg
    44341 gcagttcccg ccgagatctg tcatgatctt ctccatccag cccgggatcc ggacgccttc
    44401 gcgcgggtac atgcctggaa cgtatggctt gatgtccagc acgnnnnnnn nnnnnnnnnn
    44461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    44521 nnnnnnnnnn nnnnnnnnnn nnncgcaagg agcggcggga ccagaaggtc attttttatc
    44581 acccccacgg catagagggt catctcatct ctaatcactc tgccagtcat tgcgtgggtg
    44641 tacatgcagt tccgtacata tgaggcattc cattacctgt aaatctgata cgaaagttac
    44701 ccatatgacc cgggaaatac cgtatcatgg ctatcgtccc tacgttattt tctcattttc
    44761 cattcaccag aacgttgtaa gaaattatgt tcggacacca ttcccccgcg cctggattaa
    44821 agggaatcca cagatcggat tttttctgct gtggatcagc cagcaaatca gggtattcag
    44881 gcgccagaaa atgagcagca acatggagga tgccaaagga taaatagaaa aaagaaagaa
    44941 attattaggc aagcctaaaa gtgatcattg tgaacattcc tgtcacagag ttacgggcac
    45001 accagaaggc aaggattgtc gggctggtcg gtggacgagg gtttcagaac aggttgcgaa
    45061 gtctcgggat ccgtgaagga aaagagattt ccgtcctggt ttcgcagcct ttcgggggcc
    45121 cggttgttat cgagatagat ggcagggcaa cgacgattgg gcgtgggatg gcgagggcaa
    45181 tcatagtgga gcaagcgtga atgaaaaaga tcgtcctgat ggggaacccc aacgtcggta
    45241 aaagcgtggt gttctcccgc ctgacgggag caagggtcgt ctcatcgaac tatccaggca
    45301 ccacggtcga ctactacaag ggagagatgc tcctggaaaa aagccgggtt gagataatcg
    45361 atgctccagg cacatattcg ctgaccccta ccaacaaggc agaggaagtt gcagcggaga
    45421 tgctcaacga ggctgacctg ataataaacg tggtggatgc gacaaatctt gagcggaatc
    45481 tgtatttgac cctcgaactc attcagacac aaaaacccct gataatcgca ctcaacctct
    45541 gggacgaggc gaaaaacacg ggcatcgtca tcgatcaccg ggctcttggg acattactcg
    45601 gtgtcccggt catcccgact attgccctta gcggggaagg agtccagaat ctctgcaggc
    45661 acgtcccacg cgcgagtgcg ggtaaaatct ttgatttcac ccgggatgag gataaatggg
    45721 ctcagatcgg ggcgattatc aaacaggttc agcaggtgca ccaccgccac cccacaatag
    45781 gagaccggct ctccctcgcc tcaatcaaac cggtttccgg tattccaatc gcaataatgg
    45841 tcatcctcat ctccttctgg atcgtcaggt tcattggtga ggccctcatc aactggatat
    45901 tcgaccctct ctttgaactg tacactccta tcatcaatgc ttttagccag tggctggggc
    45961 ctggccttgc gcaggatatc ctgataggaa aacttatcga cgggcagatc gattacgtcc
    46021 agtcactggg cctcctgacc acaggattgt acgtccctat cggcatggtg ctcccctaca
    46081 ttgtcgcgtt ctatttcgtg cttgcaatcc tcgaggactc gggctacctc ccgcgccttg
    46141 ccacgctctc cgattctctg ttccaccgga tggggatgca cggccatggc atcgtccctc
    46201 ttttccttgg cataggctgc aacgtccctg caatacttgc gacaaggtgc cttgaaaccc
    46261 gaaaacagcg tttcatctct gcgacgctgg tctccatttc cgtgccatgc atggcccagt
    46321 cagccatgat attcgcaatc ctggggcctt acggggtggg atatatcctc cttgtcttcc
    46381 tcgtgctcct cacggtctac ctcactattg ggcttctgct gaatcgcgtg gtaaaaggca
    46441 tgagccccga gttgtgcctg gaaatccctc cctaccgggt ccctaacccg gcagtcatcg
    46501 tgaaaaagac atggatgcgt gtcagggggt tcctgggtga ggccatcccc tacctcttcc
    46561 tcggggtcct gctggtcaat ctcctctttg ccatagggtt tctggaatgg gttggcgctg
    46621 tgtgcgcccc tatcaccgct gggatgcttg gattaccaaa tgaagcgacc acggcactgg
    46681 tgatggggtt cctcaggaaa gaccttgccg tcggtatgct gatcccactg gatatgacgc
    46741 cggaacagct ggtgatcgcg gtgacgatgc tcaccatgta ctttccctgc gttgccacat
    46801 ttgcggtcct tttcaaagaa cttgggatca ttgacctggc gaagtccgcg atagtgatga
    46861 tctgcgcggc gttggtggtt ggaactatct tgagaattat cctgttggga ttatgaggtg
    46921 aggagtgtgc gaaaaaagac gatcgaggaa tacatcgaac tcatttatag tctcgagaaa
    46981 cgggacgggc atgcgcatac cggcctcatt gcatcagaga tgaatatcaa gcctccgtca
    47041 gtgacacaga tgctgcagaa actaaagaga gaggggcttg tttgctacca gtcctattcc
    47101 ggcgcgacac tcaccccaaa aggcaatgag atggcacgca gactcatgga gaaacaccgt
    47161 gtcatcgaag agttcctgaa gatgatcggc atcgaacaga atgttgcaga actggaagcg
    47221 tgccagatag aacatcacgt gagcataaat acagcccgtc aggtggaaag gttcgttcgc
    47281 tatctcaaaa agaagaatga tgatgaaggc tggctggacg aattcaaaaa gaccgtgatg
    47341 atctgagaga aatgttctcg gattaaattc cgattatcgg taccaggaaa actgttgcag
    47401 atggcctatt gggaaaacag aagtaatttt atatcacggt gcggtgaatg gccctgttca
    47461 gttttcattt ggagtgcgtt gacatcattc ctgctgccag gccgacgaag ccgtgagtga
    47521 aagggaccca cgttctctca aatcctgcgg cgaggagcga cctcctgaaa tccgtgatgg
    47581 tcgagatttc cggcagcctc cccgcgcgct ggcggagcat tccctcgatt acctcatcgg
    47641 gtgcaccctc ccgcttcata ccctccaccc aggaagcggt gagtattctc tcttcccagt
    47701 cctccgcggc acggaaaata tctccacata tgaaccgccc tccggcatga agaactgaag
    47761 atgctttccg ggcaacctcc tggcggtctg caggagatac gtggtgcagg cagagggagg
    47821 ag
//
LOCUS       DAOQYR010000006        46728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_876_length_46728_cov_8.817389, whole genome shotgun sequence.
ACCESSION   DAOQYR010000006 DAOQYR010000000
VERSION     DAOQYR010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 46728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46728
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_876_length_46728_cov_8.817389"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="PKM87_01425"
     CDS             complement(<1..985)
                     /locus_tag="PKM87_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28755.1"
                     /translation="MKKLAIIAFTLAILIVGMAMAADPINATVETQGISTTTGTVVLG
                     TMTNSETIVFTGSTMDIRDNPPLGAQGVNIPGTVFTAGVNPNNVWALVQTPMGSIVCL
                     PERQAVMSYTESILADNGYSEFNEIQSMDTGNKVVNQDNFKSTEQFDYVAFSDAMGRA
                     TTSESLLLDLVSQGSLAADRFICPFATGDQGFIPPYCNVYEMGSSFTGTQVSEITQAN
                     TNFIAKSADVPTEAAYTVGLSGTGSAAAWINTHVMEGRTAGYYGAEEEPVGYEYVFWN
                     YDMNTGWLGDGIFNGGGWMQGVDLVYKEKTTASGVIEAFSKSMAIQDGVRRL"
     gene            1345..2454
                     /locus_tag="PKM87_01430"
     CDS             1345..2454
                     /locus_tag="PKM87_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HNQ28756.1"
                     /translation="MATMKIRGGDLDLVEYEFNSFPPGKQIRTLGIERPASLTPVKGT
                     VKVRAPARIHLTVLDMNRFSPARPGGGGVGFAIQLYCSVEARCIERGVEIDYNRPAII
                     RHFVEVFREISGYEGGFAIRATDHQHQHVGLGSTSTIMISLAHALNYAVGSPFNSDQL
                     RRMIGYNYVEETVEGNVAFGFETGVGPAVSIHGGMAITGDELALVYHHPFAEGKNVFI
                     VIPPTDISSAGTQEFDLLMNKARVLDYRDREIKAYMIMMDLIPALEREDLEKIGNIIW
                     EIEFRGSKRAEVEHHSYAIYRYMSLLREAGLEFVGMSSVGPSIAIITSKGRDEIERIV
                     SNLGLGVAVVTKVDNMGLQITHEGGKSPSGRKKAR"
     gene            2697..3038
                     /locus_tag="PKM87_01435"
     CDS             2697..3038
                     /locus_tag="PKM87_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNQ28757.1"
                     /translation="MHEFSIAYDIYATARKAAIEHGAREVRSVSVEFGEMAMVSPEQV
                     SFLFETLVEDDPLMKGASLTVSVIPPLSRCTCGYEGTERFVCPRCGGLPELLKGREIL
                     VTNIEIEVNDE"
     gene            3035..4030
                     /gene="hypE"
                     /locus_tag="PKM87_01440"
     CDS             3035..4030
                     /gene="hypE"
                     /locus_tag="PKM87_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNQ28758.1"
                     /translation="MKVNLMHGAGGEVMGELLQILTRFEHRNAGGVGLESLDDGAVIP
                     IGGVNIVFTTDSHVVRPIFFPGGDIGRIAVCGTVNDLAMMGGKPIALSCGMIIEEGFE
                     SSDLERIVLSMDTALGECGASLVTGDTKVLERGALDGIAVNTAGIGIADRIIRDNGLQ
                     PGDRILVNGTLGDHGMTIMAERAELSLGGQLVSDVAPLWGLVEKAMAAGEIHAMKDPT
                     RGGFASAINEMGRKSNVSVRISEADLPIRRSVRSVADMLGIDPLQVANEGKVVMGVPA
                     RDAEAILEAMRSHPYGREAAIIGEVVAGSHVIMETSVGGERFIEPPVGDPVPRVC"
     gene            complement(4032..4604)
                     /locus_tag="PKM87_01445"
     CDS             complement(4032..4604)
                     /locus_tag="PKM87_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 family protein"
                     /protein_id="HNQ28759.1"
                     /translation="MGLIRDGINEVIATTHWNAAPMGIISREGTQRMVVFHGSHTAEN
                     IRRHGWVVANITFDPVVYVETAFGDIGRERFVPEKIGDLWVHRLAGAEAWAVFSSRVS
                     RETEEALVVDLTPLYEQMLTCAVHPVNRGFSSIIEATVHATRYVAKKDPALMQLIEHH
                     ASIVRRCGGKREMEALSLLQTYLPKGKGFS"
     gene            complement(4595..5413)
                     /locus_tag="PKM87_01450"
     CDS             complement(4595..5413)
                     /locus_tag="PKM87_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HNQ28760.1"
                     /translation="MRMRPLTRADRAQLAMILEVSAYPKPGNVDRCHDYETTRLEHFL
                     ASAILARPALEAAERSEGGPGALIHQAVECTSGHRGGNTHFGAFILLIPLLMGGDIPG
                     ATRVVGSTTVDDAVEFYRAFGKTEVRVIQGHELDVHDPSSIMEIRSRGMTLYDLMLFS
                     APRDMVAREWINGFEMTRRGADLIHAAGSGQQAVVEAFLGLLSLEPDTFVIKKHGPDV
                     ASKTMEKAREVREGLRDLQAFDQECIDKKINPGSIADIIIAALYIALGEGWEWD"
     gene            complement(5385..6527)
                     /locus_tag="PKM87_01455"
     CDS             complement(5385..6527)
                     /locus_tag="PKM87_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 9 domain-containing
                     protein"
                     /protein_id="HNQ28761.1"
                     /translation="MEAMRSYERYGLLLNERVVKTPIAVSAMAGIVDAEYALERAAHI
                     GAAFLGGYSIDGPTMEASRTLAAAGRPEFLYEDPVEALAAQVSRMAPSDVVCGLNLRA
                     GSPDSLAVIASQIGDSVIYEIDAHCRQEPLVRARCGEYLLHHPDLLGEYIRALKREEV
                     SVSVKMRAGVSADDLLLVKKIWGAGADIIHVDLMDFGYQKVRQIRNNSPLFVIANNSV
                     NTFEKMKDMLSHGADMVSLARQSDERTLAGLDAAISRFTDEHGWYNAPKQLCRGGDIR
                     SLAFCCMPVKNCPLIPMLEKIGLSREDYLSFKQNAVAGTPLDFGSQTCFGSLAWCCKD
                     SSPCMFREMTLRQAGLSNREYMRQKKQLADKIMQHLFYEDAATHPC"
     gene            6745..7146
                     /gene="cfbA"
                     /locus_tag="PKM87_01460"
     CDS             6745..7146
                     /gene="cfbA"
                     /locus_tag="PKM87_01460"
                     /EC_number="4.99.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin nickelochelatase"
                     /protein_id="HNQ28762.1"
                     /translation="MSKKGLLLVGHGSKLPYNKELVENTARLITGKYPEFIVKCGFMN
                     MNSPSIEESMAEFQKEAIDVLIVAPLFLAKGVHILKDIPSVIGIEEGKSKGVFSLNGR
                     TVPLLYADPIGSDPLLAELMVKNARQALKTL"
     gene            7205..8359
                     /gene="cfbE"
                     /locus_tag="PKM87_01465"
     CDS             7205..8359
                     /gene="cfbE"
                     /locus_tag="PKM87_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F430 synthase"
                     /protein_id="HNQ28763.1"
                     /translation="MKERGHSVDMVDVYRGAEGISSGQAEQNDYDLVVAPVHLDPGHP
                     LLKSIPAPVVTHHQAVRWLIGTQVPHPLVEITGARGKTTTAHALAHLMEGPGILHTSL
                     GTTRYPSRENFSKTGITPASLIDAASFAHSRSGWLIAEVSLGFTGAGTLGILASMETY
                     RCAGGKKDALEEKLRSGSALPLVLTPPGTGRCQNRLPADEIARVKDDGCQYAWEGIRG
                     EFKNPLLEHAAYRDPLALAAAAACILGIDPACLSTFSPVAGRLALSWTGKTAIIDDSN
                     SGTCAMTAAQAIRFARHATGRAGPLTLVIGKEEGSICEGFPGEAIVDLIQAECPDRVI
                     LVGREYGSLIPGSLRRKIHISCCNSLADGEALALDDGDAGMVVLAVKTWR"
     gene            8365..9429
                     /gene="cfbD"
                     /locus_tag="PKM87_01470"
     CDS             8365..9429
                     /gene="cfbD"
                     /locus_tag="PKM87_01470"
                     /EC_number="6.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase catalytic subunit"
                     /protein_id="HNQ28764.1"
                     /translation="MKYMQPRPSSIVAALYTARDLGVDVAILHGPSGCSFKHARLLEE
                     DGMRVLTTSLADHEFIFGGQQPLEAVLRYAEETFSPRRIAVVGTCVSMIIGEDLQSAI
                     RNAGISTPTISVEIHAGFPENIDGVIATLEPAAAEGWISEDELERQKRLLASANEVER
                     LRGAASRPYIEPSRGDLKHLAAERLLALTRSGRKGVAIMNAKKETAYMFADELLALHQ
                     ACPGASITYIANLEERGLPKVREDARRIRDELCEKGLFPVLCGALDEYGANGDTLGER
                     IRALSPEFAFIVGVPHAIPPRYLEGVECISVTNGPRQVEPLKDQGHRYVVVEVDLHPR
                     TLGVTHLVESELGAVIRSMA"
     gene            9426..10808
                     /gene="cfbB"
                     /locus_tag="PKM87_01475"
     CDS             9426..10808
                     /gene="cfbB"
                     /locus_tag="PKM87_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide synthase"
                     /protein_id="HNQ28765.1"
                     /translation="MKALMIAGDRSGSGKTSITLAIASLFARKMKVQTFKVGMDYIDP
                     SYLTGVTGRPCRNLDTYVMDRDQVRAVYTHGCMGADLALIEGVRGLYEGAEALEETGS
                     SASVARCLDIHVVLVVNARSITRSAAALVRGFQAFDPEIRIAGVILNNVAGDKHIRKA
                     TTAIEHYCGIPVIGAIPRMPEMELGMRHLGLMPYREGQENRKFQEKIRAVTENIGEHV
                     NLDQLLSVACATPPPQHKPDIFKPAKDPDLSIGIAYDEAFNFYYADLFDVLHAGGAQT
                     VLFSPLHDSLPDADGYILGGGYPELFLPELAARDGLKNDIRNASELGVPIYAECGGLM
                     YLTEAVILKDGWQGIQGERMYPMCGIFKGTTTMPARRVVAYVEGKSLPGSPMGESHFR
                     GHEFHYSDVSLCPGTRFCYSLSRGAGISGGSDGALMNRTLGCYTHLHPVASLPMFSHF
                     LDACRKPARA"
     gene            10854..11720
                     /gene="ilvE"
                     /locus_tag="PKM87_01480"
     CDS             10854..11720
                     /gene="ilvE"
                     /locus_tag="PKM87_01480"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HNQ28766.1"
                     /translation="MFVYIDGKFYPKEEARISVFDHGLLYGDGVFEGIRAYNGRVFRL
                     QEHLDRLYDSAKTIDLCVPLSKEEMTSAIIETLRKNNLKEAYIRPIVTRGMGDLGLCP
                     TKCPKATVIIIAVEWGAMYGDLYEKGLKAVTVSVRRNPCCSLPPNVKSLNYLNNILAK
                     IEANCKGGDEAIMFDMQGHISEGSGDNIYIIKNGVILTPPTLNNLRGITRMVVLEIAG
                     QLGIPVQETNLGYFDLYTADEVFVTGTAAEVAPIVFIDGRSIGSGRPGPITRQLMAAF
                     KTVTEREGTPVY"
     gene            11790..11864
                     /locus_tag="PKM87_01485"
     tRNA            11790..11864
                     /locus_tag="PKM87_01485"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11826..11828,aa:Glu,seq:ttc)
     gene            11976..13673
                     /locus_tag="PKM87_01490"
     CDS             11976..13673
                     /locus_tag="PKM87_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ28767.1"
                     /translation="MKIPSRNSQPGSTRIHLMAIALATFTILVLTWIALHSGIAIIYQ
                     NAYYIPIILSCYFFQRRGLYFSIGLICIYLLTLVWAFPTIEIILQGLVRVLFFLLIAL
                     LVTYLSETIQREKSRYKGIFSSSESAIIIFRKAPLEIIEANPQCTEITGYSGEELVGS
                     NVADLFLDKDRLAPSFAGVDGPFTHHETVLMRKDGDLKTVLLSTGSAEHGIGTVTMQD
                     ISLRKQAEETIKASEEKFRMLFEHSGDPIMILGMDGSILEVNSAAVKMLGYTRDELTK
                     MNARDIDTPDNTALFEKRMRELHTSGRILFETAHRTTSGEAIPIEVHATLIRFQGKPS
                     ILSTPRDISERKRAEIAIQQANRKLNLLNSITRHDILNSLTALMGYLELSLEDTKDPQ
                     MREYIHAEMRAAQTIRKQIEFTRDYQDVGVKSPGWFSVPVMIRQMRSRIEDSKARIEI
                     DLPNFEIFADPLVEKVFYNLMENAIRHGEHTTRISFTSEERDGNLVIACSDDGPGVPL
                     DKKKAIFNREYFKHSGFGLFLSREILGITGIQIQENGVPGKGARFELIIPPEGFRYP"
     gene            14114..14437
                     /locus_tag="PKM87_01495"
     CDS             14114..14437
                     /locus_tag="PKM87_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28768.1"
                     /translation="MEEKRGKFVKGAWIEEEQVSAKPGDEVKTEPIDQRIDRVTSVFT
                     TSFQQVIGLARDLVTTPEGHAHIEKQVNQAVTQIERAISDVLKMEREEKPSDSSDGKK
                     EIRIE"
     gene            14501..15217
                     /locus_tag="PKM87_01500"
     CDS             14501..15217
                     /locus_tag="PKM87_01500"
                     /inference="COORDINATES: protein motif:HMM:NF025973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HNQ28769.1"
                     /translation="MENTWEERLITFLRQPEIGYAGPGADAIARYSAAFTHQSYIREA
                     QCGEESPPPYEDYERLEFLGDRVLNLIVAEYLFSLGHHSEGSMTSCMEVVKNQHIGAI
                     VPSLGIGFTEMIRVGRNQQKTTRIIAGSFEAFIGAHYLERGLESTRRMIMRLIGDDIA
                     AFSPGDNYKKILQEELQATLGTLPVYVLHEKKGPDHRPEFVYRVLIGRRVAGEGKGRN
                     KAEATQNAALDALRARGSLS"
     gene            15389..15811
                     /locus_tag="PKM87_01505"
     CDS             15389..15811
                     /locus_tag="PKM87_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNQ28770.1"
                     /translation="MKKILAAVDGSPLTHNVIMAAVEEARCHGAELHIVHVLENAWAE
                     GDVARELAIRDLEEEAASLLSSMKAEAEAQGGEAVLHLLRGHPGDRIVDCAIDIGADM
                     VVLGSLGRSQVKRMIAGSVSTFVVTHCPITTMVVKPGS"
     gene            16026..17453
                     /locus_tag="PKM87_01510"
     CDS             16026..17453
                     /locus_tag="PKM87_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNQ28771.1"
                     /translation="MNDACSGNDGPTLRQGIIQAVVLAAIAMAVIDGIVVSIALPSIT
                     RDFSSDVALSQWIITAYLVTETSLLLIFGRLSEFTGKRTLFLAGLVLFTTSSLACGLA
                     ASLLELIVFRVIQASGSAMIFSISAAILFETSVPGSQGKVMGYIGAVTAAAGLAAPIL
                     GGFITDALGWAFIFLINVPIGALGLVLFARHSPPGERRDTSIRMDWAGSATLVIALLF
                     LVLFLVRVSSALSWSAESMVFLLVSTLSMILFLVAESRSSHPLLDLSIFRKLGFTLPN
                     LAMVCIYMAFFMLNLIGPFYLEGVMDLTPSQVGLVFLIAPLIMVIASPLSGWLYDRYH
                     LFSLPTLGILLTGVSLLLLGFVVVLQDLLLIVLLFIPLSIGISLFHSPSSTDIMRALP
                     IAKSGLASSVSATLRNLGMTLGVSVAGILFTKELYDTGYTGPVQEAAAGVLAGVIGNL
                     LFIAAALCGVGAALYLAKNILIDRV"
     gene            17555..18202
                     /locus_tag="PKM87_01515"
     CDS             17555..18202
                     /locus_tag="PKM87_01515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNQ28772.1"
                     /translation="MKILYVYAHPEPRSFNGALKDAAMVTLAVKGHDVKVSNLYAVRF
                     KAILDAQDFRIRGRHDIFNPAAEQIHGIRTGSLAPDIQEEIERVSWADLIIFQFPLWW
                     SSMPAILKGWFDRVFAQGFVVDIEAGKVYGQGLLKGKRGMLVVTCGSSEEMYSPSGVH
                     GDIRRHLSVIEHNILEFTGIEVLPPFLVFEVPSISADRAGQVLKEYESRLAALPL"
     gene            complement(18219..18614)
                     /locus_tag="PKM87_01520"
     CDS             complement(18219..18614)
                     /locus_tag="PKM87_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28773.1"
                     /translation="MEGISAEAKIGKILILVSLILGILVLLCIGVLAAIMYTAGRFFA
                     LDISLTIPLLILVPIFLVKVIGLLIGFLALNATERSDFSRAGIFAIISCVLPPLDIVM
                     LIGGIFCLVSREANETRVAGESRQKSGPE"
     gene            complement(18773..19375)
                     /locus_tag="PKM87_01525"
     CDS             complement(18773..19375)
                     /locus_tag="PKM87_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2115 domain-containing protein"
                     /protein_id="HNQ28774.1"
                     /translation="MMPGQSRGREYYHIREVCARLSKAPGRGEAGILIAREVSAYSLF
                     DLQVIMGRVRHEVHRLPPSYRNVAGPYLLSQIVDSHHSLLTTCTRGGFSGLKGPIRER
                     ELYLSYLEMVPDGCYAFDLKAEYLPQFHSPLHRLFYYLLACYVMFVLEKPGHPVGTPF
                     PGGGRVEEKKGAFYCPVREKHKDVSFALCNFCPARQDEER"
     gene            complement(19478..20368)
                     /locus_tag="PKM87_01530"
     CDS             complement(19478..20368)
                     /locus_tag="PKM87_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28775.1"
                     /translation="MTCMQDLIPRSCRGRTLIALLTLCLLGMPAAAAVLETTYMGRIT
                     ALDPLSGTITIRAESQYSCDYSGSVAVCSFAPITPMQVVGTVSDEGIFNTFRNNDPVV
                     ATILGASGGEWAGFALVAPVPGTDQWAATEVFGDPHTIPVNLAGNYEMDYSTTPDCSA
                     CTGSVCKAQSAHVVLKSEGNIVLERSLTAGNSARYSGRNDGSSVSILYLSGEADSGPC
                     SPAGAMAGMQPISNFIIHVVPPIGQAGTPGIVTHPTAAGIEPKVTPLATPAATQAPSG
                     CALVVTAFCIVAVGLRRRFS"
     gene            complement(20548..20964)
                     /locus_tag="PKM87_01535"
     CDS             complement(20548..20964)
                     /locus_tag="PKM87_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HNQ28776.1"
                     /translation="MLERLNTFEKYVYYVLIILLAFIIFVSVIELALLIAGGLVIDQT
                     YRLDNHEILTIFGFFLLVLIGVELLDTIKAYIKKQEIHVEIIVLLAVIAVARKIILLD
                     PYSDMPLSDMTLWGLGFIVLCLAAAYYLIKKAGVAT"
     gene            21283..22536
                     /locus_tag="PKM87_01540"
     CDS             21283..22536
                     /locus_tag="PKM87_01540"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNQ28777.1"
                     /translation="MAGILPVNGSQPVAGDAGYFLIESDPSGAQVIFDNRFIGVTPVT
                     YPIMGTSSPPHTITIAASGYYPHTTQYMVNPQPGETIRAYADLEPSQSFGTLVITSTP
                     GGALVTVDGGKGQQAPWTYQDIRAGSHLVQAFLSGYEPYVTITDVPAGGSITIDAVLK
                     PLSDVGTIQVKSTPGGADVYVDGIFRGSTATTVGNLAAGSHFVLLKMVGYQDWSGIVT
                     VRPNQVTILDISLQVQTGMKTGFIHIESTPSGASVFVDGVYQGVTQAGNPLDLTGIAP
                     GEHVLTLKLENYQDYTTRVLVQAGKTASVRATLTPVQAPAGSGVVQITSEPSGANIFI
                     DNRCVGITPLTIPSIPAGIHEIVIRLDGYQDYKNTFNISPGQTAQIQVALTSQIPGIP
                     LPVSSCILALCAAGIIFTFRRWQEK"
     gene            complement(22735..23136)
                     /locus_tag="PKM87_01545"
     CDS             complement(22735..23136)
                     /locus_tag="PKM87_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNQ28778.1"
                     /translation="MIRITREDFDPAQLLDEARSKELGALVSFLGVVRDDGIERMDLE
                     AYDEVAEEDLARIKDEAIARFGVLRVDIVHRVGMLAVGDNIVMIVVGAPHRKAAFQGC
                     EFIIDRIKESVPIWKKEYTADGSRWVPGEHD"
     gene            complement(23153..23434)
                     /locus_tag="PKM87_01550"
     CDS             complement(23153..23434)
                     /locus_tag="PKM87_01550"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNQ28779.1"
                     /translation="MMSVRVRAFARFRELFGDRLEVAVSSPAMIRDVVMALGQVNADG
                     LLELLDGEGRVKNSVIILVNRERITGKGREEHPVADGDEVAIYPPVAGG"
     gene            complement(23431..24195)
                     /locus_tag="PKM87_01555"
     CDS             complement(23431..24195)
                     /locus_tag="PKM87_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HNQ28780.1"
                     /translation="MADMLWVSGLMLSERERERYTRQLPLIGEDGQERLRGASVCVAG
                     AGGLGSPVLLYLAAAGVGRIRLVDPDRVERNNLNRQILHSEADIGRFKVESAAEKLRA
                     LNPDIEVEALPAAVDSGNASQMARGMDLLLDALDNYEGRYILNSAAFERKIPLVHAAV
                     SGFHGQLTTVIPGKTACLQCAMPHPPEGEDTAIIGVTAGIVGLLQALEAIKILLRKGE
                     IAGNVLLVWDGLWCRLDQIPLSRDPDCMVCGTGDYR"
     gene            24287..25105
                     /gene="larE"
                     /locus_tag="PKM87_01560"
     CDS             24287..25105
                     /gene="larE"
                     /locus_tag="PKM87_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HNQ28781.1"
                     /translation="MAERDREDRRRNNAFKALKRNLKGAGSAVISYSGGLDSSFLAAI
                     AGETIPGAVRCVILDSPLVPRRTIQEAKARAALLRIPCDVVPFPILDDPEFSRNPRNR
                     CYICKKKGSALLLEKAREYGTGQAMDGLNASDLEEFRPGTRAADEAGILHPLADCGLG
                     KQDIRSLAKEKGFLFWDQPSTPCLATRIAYGQELAVRDLQRIEAAEQSLGDRGFYRVR
                     VRMHGSLARIEVPEEEIVSLARRAADIARMMRDLGFCYCTLDLEGLRSGSMDEE"
     gene            25525..26697
                     /locus_tag="PKM87_01565"
     CDS             25525..26697
                     /locus_tag="PKM87_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HNQ28782.1"
                     /translation="MSTSRCVTEIPRITCSLFGAIKAAATLKNTVILVHGPKGCVYHV
                     NYILGLRGDRHCPVYCTCMDEHDVVFGAEERLREAIEDLDARKNPEILVVLSCCASGI
                     IGEDVQNACRVARTRSKVVGIDAGGFADDFTGGYARTLHAIVSELAGPAERQDPRRVN
                     LLGMLRAGPDLHEIRRLLSLLGLFTGAVIPAGASYDEIRCIGAASLNIVVCETAGTEA
                     ARYLQEKFKTPYIRTVYPIGAELSRRFLVDIASALGISFLPALVDDPAGNHDTSRRIR
                     IALFSGPTRALALGRFLSSHGLSPAIIILDFENPFTDEIRGAAGNECTVLVSPGWDEI
                     EDALNAHRVDLVLGGLMERPIAARLGIPLIDVMHGSLMTAGPEGGAAILRLIREHT"
     gene            complement(26874..27497)
                     /locus_tag="PKM87_01570"
     CDS             complement(26874..27497)
                     /locus_tag="PKM87_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ28783.1"
                     /translation="MEEMELLERARRAKNMGKHEEAIAAYSNALEASPQDTSIMEAMG
                     DIYYEMGRHEEALSIYQKAQNLEPHNLMVLFKKGYTLRKIHRCEDAIKCFDIALSVNP
                     GFLYALNDRGYCLNELGRYEEALACFDRSLALSPRNIRVITSKGIALRELQRLDESLA
                     CFNRALDLNAVNAFVYYQKSLTLREMGRAQEADECLKRINFPWHGKI"
     gene            27771..31295
                     /locus_tag="PKM87_01575"
     CDS             27771..31295
                     /locus_tag="PKM87_01575"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ28784.1"
                     /translation="MQNSPLSRVKIPLLAALLIITAGLAPVSADQLQANFTASPLSGP
                     HPLNVQFNDTSSGLVTSWLWYFGDNTSTNLQDPLHQYTSPGNYTVSLTVANSTGSHSL
                     LKEGYILVTNTPPVAGFSGTPLTGHVPLPVQFSDMSTGVITSYLWDFGDGNSSTDSDP
                     YHIYGAPGSYNVSLTVENDGGSNTTDAEHYITVTNAPPVSSFSANITTGVVPLPVLFT
                     DESSGEGITNWTWTFGDGGTSAVASPGYVYSTPGTYSVTLTVQNDGGTNTSTRTNYIR
                     VFNEVPIVDFMAVPTSGGEPLTVQFHDTSLGNPTTWQWQFGDGGISPLQSPSHTYQNP
                     GNYTVTLTASTSGGTSTTTKVDYIQVLQHYPVADFSAEPTLGAIPLAVNFTDLSTGTP
                     TSWSWDFGDGNTSNDQNPAHTYESAGIYTVNLTVSNGYGSDSIEKEEYISTGETPQAD
                     FNATSTEIYTNQYIEFNDLSTGSPDSYLWDFGDGSNSTYQYAWHWYPEPGNYTISLTV
                     SNEFGSDTETKADYISVTAHPPEADFQGWPRDGSFPLTVTFNQWSYGGYYENLSYLWN
                     FGDGETSTSSDYELIHTYMEAGVYNVSLTVSANGLSDTEERVNYIGNWSPPPPVAGFT
                     ATPRDGFAPLAVSFIDQSTGSPPLSFAWDFGDGESSTEQNPVHTFAGEGTYNVSLTTT
                     NAGGSSTEMKEGYIIVGGITPLKADFTSNKTSGIAPLSVQFLDASDGNPSSWFWSFES
                     GYYYVLDGAVETSALPYNPGQISSERNPVMVYQSPGNYSVMLTVSRTGESDTIEKVDY
                     IQVSPPPPVAAFNPYPSNGDAPLEVEFDGYIPWWYYYDEWVWDFGDGTTETKDYPWTT
                     HTYTDPGIYDVTLTVNSQYGSNTTTIIDCINVTQPSVPPVPNFEATPLSGNAPLDVAF
                     TDTSTGIVTSRWWDFGDGTTVWSNESADIEHTYAFPGTYTVSLMAGNAAGQETLTKTG
                     YIHVLASGLPPNAFFTMRPMSGVAPLTVSFTDRSMGAPLAWEWDFGDGTTSTEQNPVH
                     TFTTTGTFSVTLKVLNYGGSSSRSAFVFVRTMTPVYKPITPNPSQTVTASPTVTPKDV
                     SPPRPGMTPIAFFAVSKSIGFAPMTVQFTDMSFNSPTSWDWDFGDGGTSNIKNPTHTY
                     TAAGTYTAILTVENTAGSSTSSRKIYVR"
     gene            complement(31633..32130)
                     /locus_tag="PKM87_01580"
     CDS             complement(31633..32130)
                     /locus_tag="PKM87_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28785.1"
                     /translation="MPGPVARKEDDEAIAELRQYLQEIDVSIVKEFDEGEFRGFWVKF
                     GDFPILIENHKGKSYFIVAFQITLPPGQEQDTLNAYYDKKDPHFIFELTKAFTSPLTG
                     FSRILEGGRVIGFTLSRYIYPFYHDFSVKDLDRALQAVVSQGAEGIAFLKTIFPEIHE
                     TAEGS"
     gene            complement(32875..34035)
                     /gene="dinB"
                     /locus_tag="PKM87_01585"
     CDS             complement(32875..34035)
                     /gene="dinB"
                     /locus_tag="PKM87_01585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNQ28786.1"
                     /translation="MKEDRPPLSPDTKIQGDARESRAARRHDTEHRIILHVDMDSFYA
                     SVEIREHPDLAGKPVVIGADPKEGSGRGVVCTCSYEARRFGVRSAMPVSRAYHLCPQA
                     VFLPPRYPLYAKASEGVMAILRGYAERFEQVSIDEAYLDLSYLGDYPRAEAVAERIKE
                     DILTRERLTCSIGIGPGKVIAKIGSDLKKPGGLVVIPPGRVEEIIHPLSAGRIPGVGR
                     KTGEALHQKGICTIGDLAGADIQVLMEILGRWAVPLQNLARGIDESEVREHSASKSVS
                     REITFETDVGNLDLVMETLNDMARQLVGELAERNIFARTVTVKIRFSDFSTVSRARSL
                     PCHTRDFRAIVSAARELVMPLLSEKKIRLAGLRLSSLSHFDRNQKTIGDFIR"
     gene            complement(34032..34262)
                     /locus_tag="PKM87_01590"
     CDS             complement(34032..34262)
                     /locus_tag="PKM87_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28787.1"
                     /translation="MTGAHSLDRDELEKYAEKLEQRIEILGKENAALAMTLNKLMREN
                     MKLKSENKVLERRLASMNTHSEVSLNADAQKE"
     gene            complement(34381..34593)
                     /locus_tag="PKM87_01595"
     CDS             complement(34381..34593)
                     /locus_tag="PKM87_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28788.1"
                     /translation="MREDSYWFYAGLHRRFAFILPALRVKYEGDHIYLLGLHWVAVFD
                     KNYRAGSIREAGNLRLLEILPAHFAI"
     gene            34620..37244
                     /locus_tag="PKM87_01600"
     CDS             34620..37244
                     /locus_tag="PKM87_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ28789.1"
                     /translation="MLVIFIIILVLGVAGIAINYTTMARDYEENEQTLRNMTEAYLSS
                     SFHRIDTGLKMYDNTYNDQMREGFSIVMAEYNRSGGDPSRMNLERLKEATGMSVYIIN
                     QSGVIEYSTSIPDIGLDFSIIYPDFYQYLQRIWHSPGFYPDRIVTEWVSGALTKFAYM
                     PTHDHRYVIEVGLASERITAERKGLRYGDVREEAQEFNPFIEEIRIWQKQKRVVGNSS
                     YQPDDTQSEVLDTILRDRTSHEFPDPDGSRVTRWLFVDMLDPKYAADMSLIVEIVYSK
                     AKVQHDLQLLVLFHILVAGVVLLGGGGLAFILSRRLSRPIQGIVEDVDRIAKGDLEHN
                     ISRPAAEEFERIAQSINAMVETLKGSITQLQQSEDALKKSQERYRSVVETQTEMIARF
                     LPDGTHVFTNEAYCAYFNLPCTQIIGTRFKPLIPPDERAMLREYFAAFTPDRPVGTIE
                     HRIILPGGEVRWQQWNDRAIFGPSGGIIEFQSVGRDITEKKLMESALRESETNYRTLV
                     ESANSIILRFRADGTITFANPYALSFFGFRKDSLVGKNLMETIVPEFDSEGANLREKI
                     LDLAVHPERYRTSQNENVTREQKRVWVSWTNTPLFDAEGNVEEILSIGNDITRIKEME
                     DELQQLNEMLEQKVVERTSELEDANRELEAFSYSVSHDLRAPLRAIDGFSYMIISKYS
                     SQLPGDAIQYLERIRHNISYMGQLIDAILNFSRMSRQPLITQTIFPSAMVKEILDELL
                     ASKGERKVEITQNDLPPLTGDPALVRQVFSNLLSNALKFTKIRKTARIEVGSTVRESR
                     SVYYIRDNGIGFDMQYRDKIFGVFQRLHPAMEYEGAGIGLTIVQRIIQRHRGIIWVES
                     EVDKGTTFYFSFEPGPRE"
     gene            37367..37774
                     /locus_tag="PKM87_01605"
     CDS             37367..37774
                     /locus_tag="PKM87_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNQ28790.1"
                     /translation="MNPSQQTSLQVLDTLDLLVRHWNRADIPAILLHADPSCSGFGPW
                     DREHVQGHDHFRAFLERDTGRLQGLGLTGLQVEAIGTIAWVSGAFHSSSRDGTLNPEG
                     RFTAVLKGTGHAWVLVHLHFSFPHADLGEKNTR"
     gene            complement(37801..39705)
                     /locus_tag="PKM87_01610"
     CDS             complement(37801..39705)
                     /locus_tag="PKM87_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNQ28791.1"
                     /translation="MNSPMVSSRIRIGLVQASAGPDHAGNLEKVSGMVQDAFDRGAQV
                     VCLQELFSAPYFPRVHGADADRYAEHIPGETTRVLGEIARRNQGLIVAPLLEKGAGGR
                     HYNAAVIIRPDGSTGDVYRKVHIPYDPSYYEKEYFSPGSSYVVEETHFGRIAVLICYD
                     QWFPEAARTVSLMGAQVIFYPTAIGTVHGMEDPLEGDWEDAWQTVQRGHAIANAVHLA
                     AVNRVGIEGEITFFGGSFVCDAFGNIIAKAGRGEEIIVAEIDLSQNDLVREGWGFFSN
                     RRPDTYGPLVDTGTFPGAEARAPSGFRMPAEWEPHQGIWLSWPHDNDTFFDLPCVEHS
                     YLEMIQALRGRERVYLMVRDQDMRSRVEEILSCAGIEPWHVKFFVYPYCDVWFRDYGP
                     TFLVHARDSSMAMVNWRFNAWGGKYPELEGDDIIPSVLARSLHIPVFTPDMVLEGGSI
                     DVDGRGSLITTEQCLLHPNRNPSLSREGIEQVLMDFLGVSRVIWLKGGIAGDDTDGHV
                     DDVARFVDERTVVCALEKERGSDNYKILKENYEILRRTLAHDGSPLRVVPLPMPGESE
                     GSVPASYTNFYIGNGTVLVPTFNDPADRRALKTLRKVFPGRVVVGIDCSEMIRGMGAI
                     HCVSQQFPSP"
     gene            complement(39766..40845)
                     /locus_tag="PKM87_01615"
     CDS             complement(39766..40845)
                     /locus_tag="PKM87_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNQ28792.1"
                     /translation="MKTALVFHPKYLLHEQSRDHPERKERLSYTLDQLREEGLFDHPS
                     VEVFTPSEAPMEAVGRVHIPEYLRFLEQASRRGGFIDFDTNVPPGLFSHALLAAGGAL
                     RAADAVLSGEARNAFALVRPPGHHARPGTGAGFCYLNNVAIMVRHVQARGVKKVLILD
                     WDAHHGDGTQQIFYEDPTVLFTSVHQWPFYPGSGAVEETGSGKGAGFTANMPVPAGTG
                     DESYHFLFDTVIAPLAEEFSPDLIAISAGQDNHFTDPLTGLALTARGYADLMKKAVDL
                     AENLCGGRLVAVLEGGYSVEGGLPYTNLGIIAAMAGLDLRNIREPEGYGEWLTRAGDP
                     SAHAVVKEHAAALRANLAPFWQCFR"
     gene            complement(40842..42287)
                     /locus_tag="PKM87_01620"
     CDS             complement(40842..42287)
                     /locus_tag="PKM87_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HNQ28793.1"
                     /translation="MLIGMDAGGTNTDLAMVDREVTSVKVPNTLGIREVLSHARAPGR
                     LAVSTSTPLNRVLTGDPARVQTILIPGPGLFWPGGIRGAVNHRGDIVEHLDSTEVGGV
                     LASSRGDVLAITGKFSVRNPSLEQEVAARALDHYAEERIAVSHHLATLDFPGRAATTR
                     LNARMKEAVCLLAGEIGAERKDFFFFRGDGGLIPPAYAIRDPSVLYHSSTAAAALGAF
                     YLSKVEECLVVDIGGTTTDLVRLSGGKPAMETIVADGERTVTRAVAAITLPYGGDSVI
                     EETIQPRRMGNALAFGGIRPTLTDALNVTGCEIGNAGASGLLDRTSAERALEEYISLV
                     SRAVAASGARMVVGTGYLAQFLVPRIARHSGASFTIPPHAECANAVGVAVSRVTLTLH
                     ARFDSGRGAVVFNGEPQELRTLGDDEAVLDRCRAEVKQRAIAAGADPRDVEDVRVLHF
                     HAYDVVRSSFRSARIADVVVQIAPGITAEAP"
     gene            complement(42448..44331)
                     /locus_tag="PKM87_01625"
     CDS             complement(42448..44331)
                     /locus_tag="PKM87_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HNQ28794.1"
                     /translation="MYTRSLRDPERFWAEAAEEISWVRRWEKVLDTSGLPFARWFPGG
                     ILNTCHNALDRHVEEGRGDRVALVYDSPVTATIRRYTFKELQDLVARCAGGLRDLGVV
                     KGDRVVIYMPMIPEAVISMLACARIGAIHSVVFGGFAPKELASRIRDARPKVVLIASC
                     GIEVSRVIPYKPLIDEAIAIAGIPVAHCVIVQRPECPTNLADGRDREWKGFMDTASPA
                     ECVPVQATDPLYILYTSGTTGIPKGVVRDNGGHLAALLWSMRYLYGLSPGEVFWAASD
                     VGWVVGHSYIVYGPLAYGSTSILYEGKSVGTPDPGAFWRVASQHGVNVLFCAPTALRA
                     IKREDPGGKYLERHDLSRMRTLFLAGERCDPDTLAWAKARLSIPVIDHWWQTETGWAI
                     GANPVGLTMLPVKPGSCTRAMPGFDVRVLDDRGIETPVGVTGNIVIRLPLPPGCFTTL
                     WQKDDECVRTYFSAYPGFYLTGDAGFIDEDRYLWIMGRTDDIINTAGHRLSTGAMEEV
                     LASHPDVAECAVTGVADEVKGEIPVGFVVLKSGVRRENGDVVQELIQMVRDRIGPIAS
                     FKAAVVVRRLPKTRSGKILRGVMKKIADGERYSIPPTIEDQAVLGEVAESLMSIGYPR
                     RAR"
     gene            44526..45089
                     /locus_tag="PKM87_01630"
     CDS             44526..45089
                     /locus_tag="PKM87_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HNQ28795.1"
                     /translation="MEIPLVILIGISLAMDCFAVSLAASAACPSRRIRIALAFGISFG
                     VFQSGMMILGWSLGTAIVALVSGVARWIAFGILSLIGGKMIWEGVWEREAGWNADCLS
                     PATILILATATSIDSLGVGLSLGFVDSAIYLITFVVGAVAFIFSCLGAVLGGALAEKF
                     GKKFEVLGGIILIGFGVWILLGHLAAP"
     gene            45234..45629
                     /locus_tag="PKM87_01635"
     CDS             45234..45629
                     /locus_tag="PKM87_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28796.1"
                     /translation="MSNRGLVKGMPDIYQIIYQKLEKMGVLEVRQYAVIEMPPYVPLC
                     IDRLSEDVYALSQNPEVEGVMVADPDMEIRVDHARKTAEPLTLQSGETRRVVYTAPGR
                     VDLKTRIELSRFLDTWLSDLLDKGFIRHQ"
     gene            45780..46367
                     /locus_tag="PKM87_01640"
     CDS             45780..46367
                     /locus_tag="PKM87_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5591 domain-containing protein"
                     /protein_id="HNQ28797.1"
                     /translation="MPILLTEPPFYHPAFEEAHQYITGCYRIPKRPLAIFIPCALRKP
                     YSQSPSHRLFRRMISDVFDEEDYHLVIFGTCGTVPAELELMYPFAHYQYMLGKCDDPR
                     IRDDFLEIETSRLERYLRKTTHHYMRRCAYCLGVFREAMIHASERSGVPLDLLLPSNQ
                     TIETMRDPDCPFPDGSLSMKEYMDEFRNGLISMKE"
BASE COUNT        10403 a        13455 c        12618 g        10252 t
ORIGIN      
        1 acagccggcg aacgccgtcc tggattgcca tcgacttgct gaacgcttcg atcactccgg
       61 atgcggtagt cttttccttg tatacaaggt cgacgccctg catccagccg ccgccattga
      121 agataccatc acccagccaa cctgtgttca tgtcgtagtt ccagaacacg tattcgtacc
      181 caaccggctc ttcttcagcc ccataatacc cggcagtcct gccttccatg acatgggtgt
      241 taatccatgc tgctgcagat cctgttccag agaggcctac agtatatgct gcctcagtcg
      301 ggacatccgc agacttcgcg atgaagttcg tgtttgcctg agtgatctcg gagacctggg
      361 ttcctgtgaa ggaactgccc atctcgtaga cgttacagta cggcgggatg aaaccctgat
      421 ccccggttgc aaacggacag atgaaccggt cggctgccag cgatccctgg ctcaccaggt
      481 caaggaggag gctctcagag gtggttgccc ttcccatcgc atcgctgaat gcaacgtagt
      541 cgaactgctc ggtcgacttg aagttgtcct ggttcaccac cttgttgccg gtatccatgg
      601 attgtatctc gttgaattcg gagtacccgt tgtcagcgag gatggactcg gtataagaca
      661 ttaccgcctg gcgttctggt aaacatacaa tcgatcccat tggagtctga acgagcgccc
      721 aaacattatt tgggttcaca ccggcagtga aaaccgttcc aggaatattg acaccttgtg
      781 cacccaacgg tggattatct ctgatatcca tagtggatcc ggtaaacaca atcgtctccg
      841 agtttgtcat ggtgcctagg acgactgtac ctgtcgtggt tgaaataccc tgggtttcaa
      901 cggtcgcgtt gatagggtcg gctgccattg ccattcccac gatgaggatg gcaagggtga
      961 atgcaataat tgcaagtttt ttcatcgtac cccctacttt tcatactgaa ctttccattc
     1021 agtatggaat cgcatttttt ttaagtgtta tttatagatt ttccagtttt ctcaatatat
     1081 tgtaaattca ttccgaactt tcagcaccca ttagcagagg gcaacatcaa cacagttatg
     1141 aacatggggt attggcacac cacattttgg gtgggaccaa caagtgttga aatcttcatt
     1201 tgtgttatgt atcacgaata aggaagaaga ttctaatggg ctccttctct tacaaaaaag
     1261 atacccacac tgaataaaaa cgctcgatgc ataaacagga atatttactt tgatccgcag
     1321 taatctggtg tcaggtatat ccgtatggca accatgaaaa tccggggcgg agaccttgac
     1381 ctcgtcgaat atgagttcaa ctccttcccc ccaggcaagc agatcaggac cctcggcata
     1441 gaacgcccgg cttcactcac gcctgtcaaa ggaactgtga aggtcagggc cccggccagg
     1501 atccacctca ccgtgctgga tatgaacagg ttttcacccg cacgccccgg cggtggcgga
     1561 gtggggtttg ccatccagct ctattgcagc gtagaggcca ggtgcattga gaggggcgta
     1621 gagatcgact acaaccggcc agcgatcatc cggcattttg tcgaggtatt cagggagatc
     1681 tcgggatacg agggagggtt tgccatcagg gccaccgacc accagcacca gcatgtcggc
     1741 cttggttcta cgagcacaat catgatctcc ctcgcgcatg ccctcaatta tgccgtggga
     1801 tctccgttca acagtgacca gctccgacga atgatcgggt ataattacgt tgaggagact
     1861 gtcgagggga acgttgcctt cggtttcgag acaggtgtgg ggccggcggt cagcatccac
     1921 gggggtatgg ccatcaccgg cgacgagctc gccctcgtct accaccaccc gttcgcggaa
     1981 ggaaagaacg tattcatcgt gatcccccct acagacatct catccgcggg gacccaggag
     2041 ttcgacctgc tgatgaacaa ggccagggtg cttgactacc gtgaccgcga gatcaaggcc
     2101 tacatgatca tgatggacct gattcctgct ctcgagagag aagatcttga gaagataggt
     2161 aatatcatct gggagatcga gttccgtggt tcgaagaggg cggaggtgga gcaccacagc
     2221 tacgctatct accggtacat gagcctcctg cgagaggccg gtctcgagtt tgtcgggatg
     2281 agctcggtcg ggccctccat tgccatcatc acctccaagg gaagagacga aatcgagagg
     2341 attgtgagta acctcggcct cggggttgcg gtcgtcacca aggttgacaa tatgggcctc
     2401 cagatcaccc atgagggagg caaatcccct tccggcagaa agaaagcccg gtgatcacgg
     2461 aaatggtgga gcggcagggg cgtttgcgca ggacactgcg gatatccact cccccatgac
     2521 gccgcactca accgttttat cagccatttg gtccctggag tgcgacaggg aatgatttat
     2581 gccaggccct tccatatata ttggctcatc atatccctgc attccctgga tgattcgccc
     2641 gtttttcaag gtggcgtgca gctgggtttg ccgggatctc cgcgaggcat tcctgcatgc
     2701 acgaattcag tattgcgtat gatatctatg ccaccgcccg gaaggcagca atcgagcatg
     2761 gcgcacgcga ggtcaggtcg gtgagcgtgg agttcggaga gatggcaatg gtcagccccg
     2821 agcaggtcag cttcctcttc gaaaccctcg tcgaggacga cccgctgatg aagggtgcga
     2881 gcctcacggt gtcagttatt ccgccactct cccgctgcac atgcgggtac gaggggacgg
     2941 agcgtttcgt atgcccccgg tgcggcgggc tgccggaact tttgaagggg agagagatac
     3001 tggtgaccaa tatcgagatc gaggtgaacg acgaatgaag gtcaacctga tgcacggcgc
     3061 ggggggtgaa gtgatggggg aactcctcca gatactcacg cggttcgagc accggaatgc
     3121 agggggcgtg gggcttgaat cactcgatga tggtgccgtg ataccgatcg ggggggtaaa
     3181 catcgtgttc acgactgact cccacgtggt ccggcctatc ttcttccctg gcggggacat
     3241 cgggaggatc gcagtatgcg ggacggtcaa cgatcttgcc atgatgggcg gaaagcccat
     3301 tgccctgtcg tgcgggatga tcatcgagga gggcttcgag agttccgatc ttgaacgaat
     3361 cgtcctgtcc atggatacgg cactcggcga gtgcggggca agtctcgtca cgggtgacac
     3421 gaaggtcctt gagaggggtg cacttgacgg aatcgcggtg aataccgcag gcattggtat
     3481 tgccgaccgg atcatcaggg acaacggcct ccagccgggc gaccgtatcc ttgtgaacgg
     3541 gacactgggg gatcatggca tgacgatcat ggcggaacgg gccgagctct ccctcggcgg
     3601 gcagctcgta tctgatgtcg caccgctgtg ggggctcgtc gagaaggcca tggctgcagg
     3661 cgagatccat gcaatgaaag acccgacgag agggggattt gccagcgcca tcaacgagat
     3721 ggggaggaag agcaacgtct ctgtccggat atcggaggca gatctcccga ttaggagaag
     3781 cgtgagaagc gttgccgaca tgcttgggat cgaccctctc caggtcgcaa acgaaggaaa
     3841 agtggtgatg ggggttcctg cacgcgatgc cgaagcgata ctcgaggcga tgcgttcgca
     3901 cccttatggg agagaggctg ccatcatcgg cgaggtggtc gccgggtccc acgtgatcat
     3961 ggagacatcc gtcgggggcg agcgttttat cgaacccccg gtgggagacc cggttccgcg
     4021 ggtctgctga ttcaggaaaa gccctttcct tttgggaggt acgtctgcag gagcgagagg
     4081 gcctccatct cccgctttcc cccgcacctg cggacaatag atgcatggtg ctcgatgagt
     4141 tgcatcagtg cagggtcctt cttcgccacg taccgcgttg cgtggactgt tgcttcaata
     4201 atactggaaa aacccctgtt caccggatgc accgcgcagg tgagcatctg ttcatagaga
     4261 ggagtgagat ccaccaccag agcctcttct gtctcccggc tcacccttga tgagaacact
     4321 gcccaggcct ccgcaccagc gagacgatgc acccataaat ccccaatctt ctcggggaca
     4381 aaccgctccc ggccaatgtc cccgaacgcc gtctccacgt acaccaccgg gtcgaaggtg
     4441 atgtttgcga caacccagcc atgacggcgg atattttcgg cagtatggct tccgtggaac
     4501 accaccatcc tctgagtccc ctcgcggctg atgatcccca taggggccgc attccaatga
     4561 gtggtcgcaa tcacctcgtt gatcccgtcc cttatcagtc ccattcccat ccctctccaa
     4621 gggctatgta gagggcagca atgataatat ctgcgatgga tcccgggttg atcttcttgt
     4681 caatgcactc ctggtcgaat gcctggagat cgcgcagtcc ctcgcgaacc tcccttgcct
     4741 tctccatggt ctttgatgcc acgtccgggc catgcttttt gatgacgaac gtatccggct
     4801 cgagggaaag caggccaagg aacgcctcga ccaccgcctg ctggccgctc ccggcagcgt
     4861 ggatgaggtc cgcgcctctg cgtgtcatct cgaagccgtt gatccactcc cttgccacca
     4921 tgtccctggg tgcagagaaa agcatgaggt catagagggt catgccccgg gatcgtattt
     4981 ccatgatgga tgaggggtcg tgcacatcga gttcgtgacc ctgaataaca cgaacctcgg
     5041 tctttccaaa cgcgcggtaa aactccacgg catcatcaac agtggtgctg ccgacgaccc
     5101 tcgttgcacc gggaatatct ccccccatca ggaggggtat aaggagtatg aatgccccaa
     5161 aatgggtatt tcctccccgg tggccgctgg tgcattccac cgcctggtgg atgagggcac
     5221 ctgggccacc ctcgcttctc tccgcagcct ccagtgccgg gcgggctagt atcgcggatg
     5281 caagaaaatg ttcgagcctc gtagtctcat aatcatggca gcggtcgacg ttccctggct
     5341 ttgggtacgc cgagacctcc agtatcattg ccagctgcgc acggtcagca cgggtgagtg
     5401 gccgcatcct cataaaagag atgctgcatg atcttatcag ccagctgttt cttctgtcgc
     5461 atgtactctc tgttcgagag tccggcctgc cgaagcgtca tctccctgaa catgcagggg
     5521 gatgagtcct tgcagcacca ggcaaggctc ccaaaacagg tctggctgcc gaaatcaagg
     5581 ggggttccgg ctaccgcgtt ctgcttgaat gagaggtagt cctcccggga gagcccaatc
     5641 ttttcaagca tcgggatcag agggcagttc ttgacaggca tgcagcaaaa ggcaagtgac
     5701 ctgatatctc ctcccctgca gagctgcttt ggggcgttat accacccatg ctcatcggtg
     5761 aacctggaga ttgccgcatc gaggcctgcg agtgtccgtt cgtcggactg gcgggcgagt
     5821 gacaccatgt cagctccgtg ggagagcatg tccttcatct tctcaaaggt attgacggag
     5881 ttattggcaa taacaaacag tgggctgttg ttccggatct ggcgcacttt ctgatacccg
     5941 aaatccatca ggtccacgtg gatgatgtcg gcacctgctc cccatatctt ttttaccagc
     6001 aggaggtcat ccgcactgac ccccgctctc atcttgacag atacggatac ctcctctcgc
     6061 ttcagtgcgc gtatgtattc acccagaaga tcagggtggt ggaggagata ttcgccgcag
     6121 cgtgcccgga caagaggctc ctgacggcaa tgggcgtcga tctcgtagat tacgctgtca
     6181 cctatctgcg aggctatgac cgcgagcgaa tccggactgc ctgccctcag gttcaatccg
     6241 cagacaacat ctgaaggagc catgcgcgat acctgagcag ccagtgcctc gacgggatct
     6301 tcgtagagaa attccgggcg tcctgctgct gccagggtcc ggcttgcttc catggtgggt
     6361 ccatcgattg agtaaccacc caggaacgca gctccaatgt gtgcagccct ctccagtgca
     6421 tactccgcgt caacaattcc cgccatagct gagaccgcta tcggggtctt taccacccgt
     6481 tcattcagca gcaggccata cctctcgtaa ctgcgcatcg cttccatggt acgactctca
     6541 tctccggaaa aatcgcggtc cccgaagttc ttataaattt aatttttcat ggatataaaa
     6601 agttggacgg tatcgagact ttcttctccc cgccgctgtt ccggccttgc atcttggccc
     6661 gccgctgcct tgcagccttt acggggatag gcgttcggcg attatcgata accatttacc
     6721 cgtgccagtg ttactaatat acatatgagt aagaagggac ttttactggt cggacatgga
     6781 agcaaactcc cgtacaacaa ggaactggtg gagaacactg ccaggctcat caccgggaag
     6841 tacccggaat tcattgtcaa gtgcgggttt atgaacatga attcgcccag tatcgaggag
     6901 tcaatggcag aattccagaa agaagccatc gatgtcctta tcgtagcacc cctgttcctt
     6961 gcaaagggcg tccacatact caaggatatc ccttcggtaa taggcataga ggagggaaag
     7021 agcaagggtg tcttctcctt gaatggcaga acagtgcctc tcctctatgc cgatcccatt
     7081 ggatcggatc ccctgctcgc agagctgatg gtcaagaatg ccaggcaagc cctcaagacc
     7141 ctctaatggt atgaaaattc tggtactgga tactattcac ggagggagaa agttagcagc
     7201 tgcattgaag gagcgcggcc attccgtgga catggtggac gtctaccggg gggcagaagg
     7261 gatcagttcg gggcaagccg aacaaaacga ctatgacctc gtggtcgcac cagtccacct
     7321 ggatcctgga caccctctgc tgaagtcgat ccccgctccc gtggttaccc atcaccaggc
     7381 agtgagatgg ttgattggaa cgcaggtgcc acaccccctg gtagagatca caggcgcccg
     7441 gggtaagaca acgacggcgc atgcactggc gcacctcatg gaggggccgg gcattcttca
     7501 tacctcgctc gggaccacca ggtatccttc aagggaaaac ttcagcaaaa cgggcatcac
     7561 acctgcctcg ctcatcgatg ctgcatcttt tgcccattcg cgcagcggat ggctgatcgc
     7621 agaggtatcg cttgggttca ccggggccgg gacacttggc atcctggcct ctatggagac
     7681 ctaccgttgc gcgggaggaa agaaggatgc acttgaggag aaactccgtt ccgggtccgc
     7741 cctccccctg gtcctcaccc cgccaggaac gggccggtgc caaaacaggc ttcctgcaga
     7801 tgagattgcg agggtcaaag atgacggatg ccagtatgcc tgggaaggga tacgcggaga
     7861 gttcaaaaat cccctcctgg aacacgcagc atatcgcgat ccgcttgcac tggcagccgc
     7921 cgccgcctgc atcctcggta tcgatcccgc ctgcctctcg acattctctc ctgttgcagg
     7981 aagactggcc ctctcctgga ctggaaagac cgcaatcata gatgattcaa attctggcac
     8041 ctgcgcaatg actgcggcac aggcgataag gtttgcaagg cacgcgaccg gacgcgctgg
     8101 cccgctcact cttgtcatcg gcaaagaaga gggatctatc tgcgagggat tcccaggaga
     8161 agccatcgtg gacctgatcc aggcggaatg cccggaccgc gtcatcctgg tcggacgtga
     8221 atacggctcg ttgataccag gctcactgcg acgtaagata cacatctcct gttgcaattc
     8281 cctcgcagac ggagaagccc tggccctgga tgacggcgat gcgggcatgg tggtgctggc
     8341 ggtgaagaca tggcggtgaa tcccatgaaa tacatgcagc cgcggcccag ttctatcgta
     8401 gcagccctct ataccgcacg cgaccttggg gtcgacgtgg caatcctgca cggaccttcg
     8461 gggtgctcct tcaagcatgc ccgtctcctc gaggaggacg ggatgagagt cctcacgacc
     8521 tctcttgcgg atcatgaatt catattcgga ggacagcagc cgcttgaggc ggttctccgt
     8581 tacgcggaag agacattttc tccccgaagg atcgcagtgg ttgggacctg tgtgtcgatg
     8641 atcatcggcg aggatctcca gtcagccatc cggaacgcgg gcatctccac tcccacgata
     8701 tcggtcgaga tccatgccgg gttcccggag aatatcgatg gagtaattgc caccctcgaa
     8761 cctgcggccg cagaaggctg gataagcgaa gatgaacttg aacgccagaa acggcttctt
     8821 gcatccgcca acgaggtcga gcggctgcgc ggtgcggcgt cccggccgta tatcgagcct
     8881 tcccggggtg acctgaagca tctcgccgca gaacggctcc ttgccctcac acggagcggc
     8941 agaaaagggg ttgccatcat gaatgcaaag aaagagaccg cgtacatgtt cgcagacgaa
     9001 ctcctcgcac tccaccaggc ctgcccgggt gcatccatca catacatcgc gaacctcgag
     9061 gaaagggggc ttcccaaggt tcgtgaagac gcccgccgca tccgggacga actctgcgaa
     9121 aaggggctct ttccagttct ctgcggagcc ctggacgagt atggggccaa cggggatacg
     9181 ctcggagaga gaatacgggc actctccccg gaatttgcgt tcatcgtcgg cgttccgcat
     9241 gcaatccccc cccgatacct cgaaggagtg gagtgcatct cggtcacgaa tggcccacgg
     9301 caggttgaac ccctcaagga tcaggggcac cggtacgtgg tggtcgaagt ggaccttcac
     9361 cccaggacgc tcggcgtcac ccacctcgtc gagagcgaac ttggtgccgt catcaggagc
     9421 atggcatgaa ggcgctgatg atcgccggcg atcgctccgg gagcggaaag acgagcatca
     9481 cgcttgcaat cgcctccctg ttcgcaagaa agatgaaggt ccagaccttc aaggtcggga
     9541 tggactacat cgacccgtcc tacctcaccg gcgtcaccgg gcgaccctgc cggaacctgg
     9601 acacctatgt catggacagg gatcaggtca gagccgtcta tacgcacggg tgcatggggg
     9661 ccgacctggc cctcatcgaa ggagtgcgtg gcctctatga aggcgcagag gcgctcgaag
     9721 agaccgggag cagcgcctcg gtggcaaggt gcctggatat ccatgtggtg ctggtggtga
     9781 atgcgcggag catcacgcga agtgcggctg cccttgtcag gggattccag gccttcgatc
     9841 cggaaatccg tatcgcaggt gtcatcctga acaacgtggc aggagataag cacatccgca
     9901 aggccacgac tgcgatcgag cattactgcg gcatcccggt aatcggtgca atcccccgca
     9961 tgcctgagat ggaactcggg atgcgccacc tcgggttgat gccctaccgc gaggggcagg
    10021 agaaccgaaa gttccaggaa aagatccggg cggtcacaga aaatatcgga gaacatgtga
    10081 accttgacca gctcctctcc gttgcatgtg ccactccccc gcctcaacac aagccggata
    10141 tcttcaaacc cgcaaaggat ccggacctga gcattggaat cgcctacgac gaggccttca
    10201 acttctatta cgcagacctc ttcgacgtgc tgcatgccgg gggggcccaa accgtcctct
    10261 tcagccccct tcatgattcg ctccctgacg ctgacggcta catccttggc gggggatatc
    10321 ccgagctctt cctccccgaa ctcgccgcac gcgacgggtt gaagaatgac atccgcaatg
    10381 catcggagct tggagttccc atatatgccg aatgcggcgg gctgatgtac ctcaccgagg
    10441 ccgtcatact gaaagacggg tggcagggta tccagggtga gagaatgtac ccaatgtgcg
    10501 ggatcttcaa agggacgacg accatgcctg caaggagggt ggtcgcgtac gtggaaggga
    10561 agagccttcc aggctcccca atgggtgaga gccatttcag gggtcacgag ttccattatt
    10621 ccgacgtgtc tctttgtccc gggacccggt tttgctactc gctctcgcga ggtgccggta
    10681 tatcaggggg atcagacggg gccctcatga acaggacgct tgggtgctac acgcaccttc
    10741 atccggtggc aagccttccc atgttctccc atttccttga cgcctgccga aagcctgcga
    10801 gggcctgatt caatcagcag gtaaaaaata ccaggagaat aaacattcat ttcatgttcg
    10861 tctatatcga tgggaagttc tatcccaaag aggaggccag gatctctgtc tttgaccatg
    10921 gcctcctcta tggagacggg gtgttcgagg ggataagggc atacaacggc cgggtgttcc
    10981 gcctgcagga gcaccttgac cgcctgtacg attcggcgaa gaccatcgac ctctgtgtcc
    11041 ccctctcaaa agaggagatg accagcgcga tcatcgagac gctccggaag aacaatctga
    11101 aggaggcgta tatccgaccc atcgtgaccc gtggcatggg agatcttggc ctctgcccca
    11161 ccaaatgccc aaaagcgacg gtcatcatca tcgcagtgga atggggagcc atgtatgggg
    11221 acctctatga gaagggacta aaagctgtca ccgtctcggt gaggcgtaac ccctgctgtt
    11281 ccctgccgcc gaacgtaaag tccctcaatt acttaaacaa catccttgca aagatcgagg
    11341 caaactgcaa gggaggagac gaggccatca tgttcgacat gcaggggcac atctccgaag
    11401 ggtccggaga taatatctat atcatcaaga acggagtcat cctgactcct cccaccctga
    11461 ataacctgag aggaatcacc cggatggtgg tgctggagat cgccgggcag cttgggattc
    11521 ctgtgcagga gaccaacctt ggctatttcg atctttacac cgcagacgag gtctttgtca
    11581 ccggaaccgc ggcagaggtg gcgcccattg tctttatcga cgggaggagc atcggttcag
    11641 gccggccggg ccccatcacc cggcagttga tggctgcgtt caagaccgtc accgagcgag
    11701 agggcacccc cgtctattga gaaaaactcc ccccatctac cggctccaca acccttttta
    11761 ccgtgaaccg ccaactaaaa agagcaattg ctgctatagt gtagtccggc caatcatgcc
    11821 ggcctttcac gccggcgact ggggttcgaa tccccatagc agcactgttt ttcacatttt
    11881 ttcccccctt taacttagcc atatcctgtt ttattagaga aaacgacgaa tattttttta
    11941 taataatcat tccaacaccc gtaggatctc ccttcatgaa gataccaagc cggaattctc
    12001 agcctggatc tacccgcatc catctcatgg ccatcgcatt ggcaacgttc accatcctgg
    12061 tcttgacatg gattgctctt cattccggta tcgctatcat ctaccagaat gcctattata
    12121 tccccataat cctctcctgc tatttttttc aacggagagg gctttatttc tccatcggcc
    12181 tcatctgcat ctatctcctg acgctcgtat gggccttccc cacaattgaa atcatactcc
    12241 agggactggt ccgggtgctc ttcttcctcc tcattgcatt gctggtaacc tatctctctg
    12301 agaccatcca gagagagaag tcaaggtaca agggtatctt ctcctcctct gaatcggcca
    12361 tcatcatttt caggaaagcc ccccttgaaa tcatcgaggc aaacccgcaa tgcactgaga
    12421 ttaccggtta ttccggtgaa gaactggtcg gttccaacgt cgctgacctt ttcttagaca
    12481 aagaccgcct ggcaccatcc ttcgctggag tggatgggcc tttcacgcac catgaaacag
    12541 tgctcatgcg taaagacgga gacttgaaga ccgtgctcct ctcgacaggc tctgcagaac
    12601 atggaatcgg cactgtcacc atgcaggaca tctcgcttcg caaacaggct gaagaaacca
    12661 taaaagcaag tgaagagaag ttccgtatgc tctttgaaca cagcggggac cctatcatga
    12721 tccttggcat ggacggctcg attcttgagg taaacagcgc agcagtgaag atgctcggct
    12781 acacgcgtga cgaattgacg aaaatgaatg cccgcgatat tgataccccc gacaacacgg
    12841 ccctgtttga gaagcggatg cgagaactgc atacatccgg aagaatcctc ttcgagactg
    12901 cccaccggac gacatccggg gaggcaatcc caatcgaagt gcatgccacg cttatcaggt
    12961 tccagggcaa accttcaatc ctcagcaccc cccgggacat atcagagcga aaaagggcag
    13021 agattgccat ccagcaggca aacaggaaac tgaacctctt aaacagtatt acacgccacg
    13081 acatcctcaa ttccctgacg gcactcatgg gttacctcga actctcgctg gaagatacga
    13141 aagatcccca aatgagagag tatattcacg cggagatgcg ggcggcgcag accatccgga
    13201 agcaaatcga gttcacgagg gattaccagg acgtaggtgt gaaatctccc gggtggttct
    13261 ctgtccctgt catgatccgg caaatgagaa gccgcattga agattcaaaa gcgcggatcg
    13321 agatagacct tcccaatttc gagatattcg cggatccgct tgtcgaaaag gtcttctaca
    13381 atctcatgga gaatgccatc cgccatggag aacacaccac gcggatcagc ttcacttctg
    13441 aagagcgcga cggcaatctt gtgattgcct gctccgatga cgggccggga gttccactcg
    13501 acaaaaagaa ggctattttc aaccgtgagt acttcaagca ttcggggttt gggctcttcc
    13561 tctccaggga gatcctggga attaccggta tacagatcca ggagaacggg gttccgggga
    13621 aaggggcgcg gttcgaactg ataattcccc cggaggggtt caggtatccc tgatacccga
    13681 cgctgccctc accggactcc tgcagcagcc gggccttgag gtgttttcct caaaaaaaca
    13741 catgattctc atcgagacac acgtatacaa aatgaaaatc gcggatgcga ttttcacatt
    13801 aaaaaagggg gcaagagact tccccatggc gcccaattca ggaccaggga acgatgcagc
    13861 acgctgtgag aaatccatcc ctgcaagagc tgatggccgg ctccctgcct ggcgcatcag
    13921 atccaggaac aatttttctc taaagcacgc atatcacact gatacctggc tgaaccatct
    13981 cacgggggct tggcaggcac cctcctgcca aaggaacagg ttcaagaaga ggcctctccc
    14041 aatctcgagc aggatacaat cagagcgggt atggagcgcg aggcaggccg gggaattaca
    14101 cggcaggcag atcatggaag agaaacgtgg aaaatttgtc aagggagcat ggatcgaaga
    14161 ggaacaggtg agcgcaaaac cgggagatga ggtgaaaacc gagcccattg atcagagaat
    14221 cgaccgggtt acctccgtgt tcacgacctc ttttcaacag gttatcggtc ttgcgcgcga
    14281 ccttgtcact actccggaag ggcatgcgca tatcgaaaag caggtcaacc aggcagtgac
    14341 gcagatcgaa cgtgccatct ctgatgtatt gaaaatggaa cgcgaagaga agccttctga
    14401 ctcatccgat ggtaagaagg agatacggat cgaataggca atcaatgcga caatgtatct
    14461 tttttcctga tgcatgccgg cgggattaaa gcgggatcca atggagaaca cctgggaaga
    14521 acggctcatc accttccttc gtcaacccga aatcgggtat gccggtcctg gggcggatgc
    14581 aattgcacgg tattctgcag cattcactca ccagtcgtac atcagggagg cgcaatgcgg
    14641 agaagaatct cctcctcctt atgaggatta tgagaggctt gagtttctcg gcgacagagt
    14701 cctgaacctt atcgttgcag agtacctctt ctctctgggg catcacagcg aagggagcat
    14761 gacctcgtgc atggaggttg tgaagaacca gcacattggt gcaatagtgc cctcactcgg
    14821 tattgggttc acggagatga tacgtgtggg gcggaaccag cagaagacga caaggatcat
    14881 cgcaggctct tttgaggcgt tcatcggagc tcactacctt gagagagggc ttgaatccac
    14941 gaggagaatg atcatgcgcc tcattgggga cgacattgcc gcattctctc caggcgataa
    15001 ctacaagaag atcctgcagg aggagcttca ggcgaccctt gggacgctcc ccgtctacgt
    15061 ccttcatgag aagaagggtc cggaccaccg ccctgagttt gtatacaggg tcctcatagg
    15121 cagaagagtc gctggcgaag gaaagggtcg caacaaggcc gaggctaccc agaacgccgc
    15181 cctggatgcc cttcgggccc gtggaagcct ttcctgaacc gaaattacga caaggccata
    15241 attacatggc ctcgaaatgg ttgccgtggc atgggagctg cggagcactt atgtggtccc
    15301 atgtcactag acatacgtat acgggactgt ttcccacgta agatcgtgcc tggagcagtt
    15361 tcttcaccgc agggggttga tcaggctgat gaaaaagata cttgcggcag tggacggatc
    15421 cccgctcact cacaacgtga tcatggctgc agttgaggag gcaaggtgcc atggtgcgga
    15481 acttcatatt gtccatgtgc tggagaacgc ctgggcagaa ggagacgttg caagggaact
    15541 ggcaatacgt gaccttgagg aggaggctgc atccctcctc tcatcgatga aggcagaagc
    15601 agaagcacag ggtggcgagg ccgtattgca ccttctcagg ggtcatcccg gagaccggat
    15661 cgttgactgc gccattgata ttggtgctga catggtcgtt ctcgggtcat tgggcaggag
    15721 ccaggtcaag cggatgatag ccgggagcgt atccaccttt gtggtcaccc actgccccat
    15781 caccaccatg gtggtaaaac ccggttcctg aatgccgctc ccgcgtgaag agcatgagga
    15841 gagagtggtc tttcctctgc tcctttggga gaatccgcgc cagcccggca attatccaaa
    15901 cctgccacac gccagacaag tcaccccatg aaccccgggc ctttgatccg cttgcaatcc
    15961 ctttgcctgc tgagagggaa gaatttcatc gtcagaagtc taaaactctg cacgattatt
    16021 catcgatgaa cgatgcttgc tccgggaatg acggtcccac cctgcggcag ggaatcatcc
    16081 aggcagtggt acttgcggca atcgcaatgg cagttatcga cgggatcgtg gtgagcatcg
    16141 ccctcccatc gatcacgagg gacttctctt ccgacgtggc cctttctcag tggatcatca
    16201 ccgcctacct cgttaccgag acgagtctgc tcctcatctt cgggagactc tcggagttca
    16261 ctgggaagag gacactcttt ttggccgggc tcgtgctctt caccacgagt tctctggcct
    16321 gcggccttgc agccagcctg ctcgagctca tcgtatttcg cgtgatccag gccagcggtt
    16381 ccgccatgat attctccata agcgcggcca tcctctttga gacttcggtc cccggcagcc
    16441 aggggaaggt gatgggctac atcggggcgg tcacggccgc ggcgggcctc gcggctccga
    16501 tcctgggcgg tttcatcacc gacgccctgg gctgggcatt tattttcctg ataaacgtcc
    16561 ccataggggc tctcggcctg gttctcttcg cccgccattc tccacccggt gaaaggcgcg
    16621 acacgtccat acggatggac tgggccgggt ctgctaccct tgtcatcgcg ctcctctttc
    16681 ttgtactctt cctcgtccgg gtgtcatctg ctctctcgtg gtctgccgag tcaatggtat
    16741 tcctccttgt atcaaccctc tccatgatcc tcttcctggt cgcagaatcc aggagctccc
    16801 atccactcct tgacctctcc atattcagga agctcggttt tactcttccc aatcttgcca
    16861 tggtatgcat ctacatggca ttcttcatgc tgaacctcat cggacctttc tatctcgagg
    16921 gggtgatgga cctgacacca tcccaggtag ggctcgtctt cctgatcgcc cctctgatca
    16981 tggtcattgc ttcccccctc tcgggctggc tgtatgatcg atatcatctc ttttcactcc
    17041 ccacactggg aatcctgctc accggggttt cactgcttct cctcggcttc gtggtggtgc
    17101 tccaggatct cctgctgatc gtcctcctgt tcatcccgct ctcgatcgga atctcgctct
    17161 tccacagccc aagcagcacc gatatcatgc gggcgcttcc catcgcaaag tcgggacttg
    17221 catccagcgt gagtgcaact cttcggaacc tggggatgac actaggcgtc tctgtggcag
    17281 gaatactctt cacaaaagaa ctttatgata caggatatac cggaccggtc caggaagcag
    17341 ccgcgggcgt tcttgcagga gtgattggaa accttctctt tattgcagcg gcgttgtgcg
    17401 gtgtaggagc cgcgctttac ctggcaaaga acatccttat cgaccgtgtc tgaagaagcg
    17461 gaaaatttac aatcctgccc ccatgcgcaa tcccgaaact gggaggggat gctattatga
    17521 aggattcccg caatctccct ttcagggatt ccacatgaag attctatatg tctatgctca
    17581 cccggaacca agatcattca acggtgcact caaggatgct gccatggtca cgcttgccgt
    17641 aaaagggcac gatgtgaagg tctcgaatct ctatgcggtc aggttcaagg ccattctgga
    17701 cgcacaggat ttccggatca ggggccggca cgacatcttc aaccctgcag cggaacagat
    17761 ccacgggata cgcaccggga gcctggctcc cgacatacag gaagagattg agcgtgtgtc
    17821 atgggccgat ctcatcatct tccagttccc cctctggtgg tcttcgatgc ccgccatact
    17881 caaaggatgg ttcgaccgcg tatttgccca gggattcgtg gtggatatcg aggccggcaa
    17941 ggtatacggg caaggccttc tcaaggggaa gagagggatg ctggtggtga cctgtggctc
    18001 gtcagaggag atgtactctc cttcgggggt gcatggtgac atccggagac atctctcggt
    18061 tatcgaacac aacatcctcg agttcaccgg gatcgaagtg cttcccccgt tcctcgtatt
    18121 cgaggtccca tcgatctctg ccgatagggc cgggcaggtc ctgaaagaat atgagagccg
    18181 gctcgccgca cttccccttt gaacctattg ggggaggttc attccggtcc gctcttctgg
    18241 cgactttcac ctgccaccct ggtctcgttc gcttccctcg agacaaggca gaatatcccg
    18301 cctatcagca tcacgatgtc cagggggggc agcacgcagg agatgatggc aaatatccct
    18361 gcccgtgaaa aatcactcct ctctgtggca ttgagagcaa gaaaacctat gagaagcccg
    18421 attaccttga caaggaagat cggtaccagt atcaggaggg gtattgtcag gctgatatcc
    18481 agcgcaaaga atctcccggc cgtgtacatg atcgcggcga gcaccccgat gcacaggagc
    18541 acgaggatcc cgagaatgag tgagaccagg atgaggatct tgcctatctt ggcctctgcg
    18601 cttattcctt ccatgttcaa catctcatct gcaccactct cacatttcat ccatgcatcc
    18661 ccttgaatct attgctgaaa gactggacta aaagcccgtg tgtgaaggga atctgcccat
    18721 gggataaaag gacagcggtt gcaggaacaa tacggctttg aaaatagcgg cactacctct
    18781 cttcatcctg ccttgcaggg cagaaattgc agagggcaaa tgagacatcc ttgtgttttt
    18841 ccctgacggg gcagtagaag gcaccttttt tctcctccac cctgccccca ccgggaaatg
    18901 gtgtcccgac aggatgtccg ggtttctcca ggacgaacat gacatagcat gcgaggagat
    18961 agtagaacaa cctgtgcaga ggggaatgga actgtggaag gtattcggcc ttcaggtcga
    19021 atgcatagca gccatccgga accatttcta gatacgagag atacagttct ctctctctta
    19081 taggcccttt caagccgctg aatccaccgc gcgtacacgt agtgagaaga gagtggtggg
    19141 agtccacgat ctgtgagagc agataagggc ccgccacatt ccgatacgag ggaggaaggc
    19201 gatgcacttc atgcctgacc ctgcccataa tcacctggag atcgaacaga gagtatgcag
    19261 atacctctct tgcgataagg ataccagctt cgccacggcc cggggctttt gacagcctgg
    19321 cgcacacttc ccggatgtga taatactccc gtccccggga ctgccccggc atcatgactg
    19381 gccccgggag gatgtggctc gattgaaagc cgtcccaaaa gtgtgctgac gaaaaattat
    19441 ggggaccggg ttcatgataa ctttagtgta caggcggtca gctgaatcgt cgccttaagc
    19501 ccacagccac aatgcagaat gcggtaacga ccagggcaca cccggagggt gcctgcgtgg
    19561 cggcgggtgt agccagcggg gtaaccttgg gttcaattcc cgcagcagtc gggtgcgtga
    19621 caatccccgg agttccggcc tgcccgatgg gcgggacgac gtgaatgata aaattggaga
    19681 tcggctgcat tccggccatg gcccctgcag gtgaacaggg gcccgagtct gcctcaccgg
    19741 aaagatacag gatactcacg cttgaaccgt cgttcctccc tgaatatcgt gcagaatttc
    19801 cggcagtcaa tgatctctcg agcactatgt tcccctcgct cttcaggaca acatgcgcgg
    19861 attgagcctt gcagaccgat cctgtgcatg cgctgcagtc aggggttgtg gaatagtcca
    19921 tctcgtagtt gcccgcaaga tttacaggga ttgtgtgcgg gtcgccgaat acctcggtcg
    19981 ctgcccactg gtccgttccg ggaaccggcg caacaagggc gaaccccgcc cactcgccgc
    20041 cgcttgctcc gaggattgtg gcgaccacag gatcattatt gcggaaggtg ttgaaaattc
    20101 cctcgtcgga tacagtcccc accacctgca tgggggtgat gggagcgaaa ctgcagacag
    20161 cgaccgatcc cgaatagtcg caggagtact gtgattccgc acgtatggtg atggttccag
    20221 aaagcggatc gagtgctgtg attcgcccca tgtatgtcgt ctccagcact gcggctgcgg
    20281 caggcatccc aaggaggcag agagtgagga gcgcaatgag agtgcgaccc ctgcaggacc
    20341 ggggtatgag atcttgcatg catgtcatgg ttgaggtgag atatggtgcg gttgaaataa
    20401 aaaaccagat gatatagtgg aatggagagc ctggcaggtg tccttcttga aggtattcga
    20461 gagctgaaca agagcctgtg gccgaggcac agggtttctt tgcggcatgg aatgcggccg
    20521 gcgcatgaga aggatgggcc tgtcccctta cgtcgcaact cctgcttttt tgatcaggta
    20581 atatgctgcg gcgaggcaga gaacgatgaa cccaaggccc cacagggtca tgtccgacag
    20641 gggcatatcc gagtacgggt ccaggaggat gatcttcctt gcaaccgcga tgactgccag
    20701 gaggacgatg atctccacgt ggatctcctg tttcttgatg tatgccttga tggtgtccag
    20761 gagttccacc ccgatcaaca ccaggaggaa gaagccgaat atcgtcagta tttcgtggtt
    20821 gtccagccgg tatgtctggt caatgacgag gccgccggca atgaggaggg caagctcaat
    20881 gaccgaaaca aagataatga acgcaaggag gatgataaga acgtaataga cgtatttctc
    20941 gaatgtattc agtctttcca gcatcgattc cctcttttta ccctgggccg gtcactgttc
    21001 ttctgcattg cagggactgc ggtccttttg cacccgcggc acccatattc atacgggccg
    21061 gttgtccata tgccgtggag cctggcagga tgttcactcg tacatgaacc ttcccgtcct
    21121 ataaactctc tgccacacct ggttggacgg tcttttaatc aggactttgg actgctcttc
    21181 ccctggggcg ccgcccatga atttttgtag aagaagagag agaggtgtgc atatatgcga
    21241 atgaacacac atgtgctcat atttctcttt tttttatctg ccatggcagg cattcttcct
    21301 gtcaatggat cccaaccggt cgcaggcgat gctggctatt tcctgataga atcagatccc
    21361 tctggtgcgc aggtcatctt tgacaaccgc ttcattggag ttactccagt cacctacccc
    21421 ataatgggaa cgtccagccc cccgcatacc atcacgattg ccgcaagcgg gtactacccc
    21481 cacaccaccc agtacatggt caatccacaa cctggagaga ccatcagggc atacgccgac
    21541 ctggaaccca gccagtcatt cggcaccctg gtgatcacgt ccacaccggg tggtgcactg
    21601 gtcactgtcg atggcgggaa ggggcagcag gcaccctgga cgtaccagga catacgcgcc
    21661 ggcagccacc tggtacaggc ctttctctca ggatacgaac catatgtcac gatcaccgat
    21721 gttcccgcag ggggcagcat cacgattgac gctgtattaa aacccctctc ggacgtgggg
    21781 accatccagg tcaaaagcac tccgggggga gccgatgtgt atgtcgacgg catcttccgg
    21841 gggtcaactg caaccacagt cggaaatctg gcggcgggat cgcattttgt ccttctgaaa
    21901 atggtcgggt accaggactg gtcaggaatc gtgaccgtgc gccccaacca ggtgaccatc
    21961 ctggatatat ccctgcaggt ccagacgggg atgaagacag ggttcatcca catcgaatcg
    22021 actccgtcag gggcatccgt gttcgtcgat ggagtgtacc agggagtcac ccaggctgga
    22081 aatcccctcg atctcacggg aatcgcaccc ggagagcatg tcctgacgct taaacttgag
    22141 aactaccagg actacaccac cagggtactg gtgcaggcag ggaagacggc gagtgtccgg
    22201 gcaaccctca cgccggtaca ggcacctgcc gggtccggtg tggtgcagat cacttcggaa
    22261 ccttccgggg caaatatctt catcgataac cggtgcgttg gcatcactcc gttgaccatc
    22321 ccctcgattc cggccggaat ccacgaaatc gtcatacggc tggacggata ccaggactac
    22381 aagaatacct tcaacataag ccccgggcag accgcccaga tccaggtcgc gctgacttca
    22441 cagataccgg ggatcccgct ccctgtctct tcatgcatcc ttgcgctttg tgccgcagga
    22501 atcatcttta cgttccggag atggcaggaa aaatagattc ccccttttta ctatcaaaat
    22561 ggctccgcat ttttcatttt gtatgcgtgc gtcctaaaaa agaatcatgt aatttttatg
    22621 gggatgagtt cctggcaggg atgggtgtaa atgacgcctt ttttagttgt cctctggcaa
    22681 cacgcttttt ttcattgagg agggcgctcc tttttcactt accttccgga gagttcaatc
    22741 gtgttcaccg ggcacccatc gcgaaccatc agcggtgtat tctttcttcc agatcgggac
    22801 tgattccttg atgcggtcga tgatgaattc gcatccctgg aatgccgctt tccggtgagg
    22861 agcccccact acgatcataa cgatgttgtc accgacggca agcatcccta ccctgtgtac
    22921 gatatccaca cgcaaaacgc caaaccgggc aatagcctca tcctttatcc gggccaggtc
    22981 ctcttctgcc acttcgtcgt aggcttcaag atccatccgc tcaatcccgt catctctcac
    23041 cacgccgagg aaactgacga gggccccgag ttctttgctt cgagcctcgt caaggagttg
    23101 tgccgggtcg aaatcttccc ttgttatccg aatcatttca tgcaccctgc cgtcacccac
    23161 cggcgactgg aggatatatt gccacttcgt cgccatccgc aacagggtgt tcctccctgc
    23221 ctttcccggt gatccgctcc ctgttcacga gaatgatgac cgagttcttc accctgcctt
    23281 caccatccaa aagttccaaa agaccgtctg cattcacctg tcccaaggcc atcaccacat
    23341 cccttatcat ggctggcgaa gataccgcca cctcgaggcg gtcaccgaac agctccctga
    23401 accgtgcaaa agccctgacc ctgacactca tcatctgtaa tcacccgttc cacacaccat
    23461 acaatcgggg tcccttgaga ggggtatctg atcgagcctg caccataagc catcccagac
    23521 cagcaggaca ttccctgcga tctctccttt acggagcagg atcttgattg cttccagagc
    23581 ctgcagcaga ccgacgatgc cagcggtgac cccgatgatc gcagtatcct ccccttccgg
    23641 ggggtggggc atcgcgcact gaaggcatgc agtctttccg gggatgacag tcgtcaactg
    23701 tccatggaac ccgctcactg ccgcatgaac aagtgggatc tttcgttcga atgcggcgct
    23761 gttcaggata tagcgtccct cgtagttgtc aagtgcgtcc aggagcaggt ccatgccgcg
    23821 tgccatttgg gatgcattcc cagaatccac tgcagcgggc agggcctcga cctctatgtc
    23881 agggttaagc gcacgaagtt tttccgcggc agattccacc ttgaacctgc cgatgtccgc
    23941 ctctgagtgc aggatctgcc tgttcaggtt atttctctcc acccggtctg gatcgacaag
    24001 tcgtatacgg ccgacaccgg cggcagccag gtagagcagg acaggtgagc cgagaccgcc
    24061 tgctcccgcg acacatactg atgcaccgcg gaggcgctcc tgcccatcct cccctatcag
    24121 gggcaactgg cgcgtgtatc gttcgcgctc cctctccgaa agcatcagtc cgctcaccca
    24181 caacatgtcc gccatttcag tacaggcggg cactgtagag actatatagt catcctgaaa
    24241 cataatggtg cgtaaccgca tcatcaatgc tcgcggggag aggggagtgg cagagagaga
    24301 cagggaggat cgaagaagga acaacgcatt caaggcactc aaaagaaacc tcaagggtgc
    24361 cggaagcgcg gtcatctcat attcaggcgg cctggacagc tcgttcctgg cagccattgc
    24421 cggggagacg attccgggag cggtacgatg cgtgatcctc gattctcccc tcgtcccgag
    24481 gcgcacgatc caggaagcaa aagcccgggc cgcacttctc cggatcccgt gtgatgttgt
    24541 accattcccg atcctcgatg acccggagtt ttcccggaac ccaagaaacc gctgctacat
    24601 ctgtaaaaaa aagggatctg ctctcctcct ggaaaaggca cgcgaatacg gaacagggca
    24661 ggcaatggac ggcctgaatg catctgacct ggaggagttc cgccctggca cgagggctgc
    24721 agatgaagcc gggatccttc atccccttgc ggactgcggc ctgggaaaac aggacatcag
    24781 gtctcttgca aaagaaaagg ggtttttatt ctgggaccag ccttcaaccc cctgcctcgc
    24841 aacccggatt gcgtacgggc aggaactggc cgttcgggac cttcagcgaa tcgaggcggc
    24901 agagcagtcc cttggagacc gcggctttta ccgcgtgaga gtgcgcatgc atggaagtct
    24961 tgcccgcatc gaggtcccag aggaagagat tgtcagcctg gcccggagag ccgccgacat
    25021 agcgcggatg atgagggatc ttggtttttg ctactgtact ctggacctgg aaggcctgcg
    25081 gagcggtagc atggacgagg aatgacgcca tgtgttcgct tgcgaaaggt gcatggggct
    25141 gggtggaggt tattctcttc ttttttcgtg aatgtggaga tccgtccccc tttcttcctc
    25201 cctgctctcc atttcttttg ctttccgaaa aataaggctc aaatgtccct gacatgacct
    25261 tccttgcatg ggtgcgaacg atgaaaacga ccggccgaac tgccatgcat tccgttaccc
    25321 ggactgtaat gctctcctgc gtgggaagac gccgaatccc ttacgccccc ggaaagaagg
    25381 tgcgggagag gttggaaacc atgcaaggga aaagcgccat ttcgtcaaag aggcttcctg
    25441 gtattgttca tgtctatgaa accatcccgt cacaccctca accgataagc tataacatcg
    25501 atccctgacc catagacttc tgttgtgtcc acttccaggt gcgtcactga gataccccgc
    25561 atcacctgca gcctcttcgg tgcaatcaag gccgcggcca ccctgaaaaa taccgtaatc
    25621 ctcgtgcatg gaccaaaagg ttgcgtctat catgtcaact atatcctggg gctccgcggt
    25681 gaccgccatt gccccgtcta ctgtacctgc atggacgaac acgacgtggt cttcggcgca
    25741 gaggagcgat tgagagaggc gatcgaggat cttgatgcac ggaaaaatcc ggagatactg
    25801 gtggtcctct cctgctgtgc atcggggata atcggggagg atgtccagaa cgcctgcagg
    25861 gtcgcccgca cccgttcgaa agttgtcggc atcgatgcgg gagggtttgc tgatgatttt
    25921 accggaggat atgcgaggac actgcacgca atcgtctctg aacttgccgg acctgctgaa
    25981 cggcaggacc cgcggagggt caacctcctg gggatgctca gggccgggcc cgacctgcac
    26041 gagattcgcc ggctcctctc gctgctcggt ctttttacag gcgcggttat ccctgctggt
    26101 gcatcatatg acgaaatacg ctgcattggc gctgcatctc tgaatatcgt ggtatgcgag
    26161 actgcaggca ccgaggctgc ccgttacctc caggagaagt ttaaaacccc gtatatacgg
    26221 acagtgtatc ccatcggcgc cgaactgagt cgcagatttc tcgtggacat cgcttctgcc
    26281 ctcggtatct ccttcctccc tgctctcgtt gatgatcccg caggcaatca tgatacgtcg
    26341 cgaaggatca ggatagcact cttctccggc cctaccaggg ctctcgccct tggccgtttc
    26401 ctctcctcac atggcctgtc gcccgcgata atcatcctcg atttcgagaa cccgttcacc
    26461 gatgagatca ggggtgcagc aggaaatgag tgcacggttc tggtctctcc tgggtgggat
    26521 gagattgaag atgccttgaa cgctcaccgg gtcgacctcg tgctcggcgg actgatggag
    26581 cgacccatcg ccgcgagact tggaattccc ctgatcgacg tgatgcacgg gagcctaatg
    26641 actgccggcc cggaaggggg ggccgccatc ctccgcctga tcagggagca cacctgaacg
    26701 ggcgcattcc acccaattcg cttcaattca atcacgcgat gggaagcctg caggaacggg
    26761 acggaaggga agcgatcgcc tcgccgggag gttcatgcca gagaagaaac ggcccggttg
    26821 cccggtcgct tcggagatcc catcatgcca tttttttggg aggggaaccg ggactatatc
    26881 ttcccgtgcc agggaaaatt aatcctcttc aggcactcgt cggcctcctg cgctctcccc
    26941 atctcccgga gcgtgaggga cttctggtaa tatacaaacg cgttcaccgc attcagatcg
    27001 agggcacggt tgaagcacgc cagagactca tccagccttt gaagttcacg gagtgcgatt
    27061 cccttgctcg tgatgacccg gatattacgc ggagataaag cgagcgagcg gtcaaaacat
    27121 gcaagagcct cctcatacct ccccagttca ttcaggcagt aacccctgtc attcagtgcg
    27181 tataaaaagc cgggattgac cgagagcgcg atatcgaagc acttaattgc atcctcacac
    27241 cggtggatct tccgcagcgt atatcctttc ttgaagagga ccatgaggtt gtgtggttcg
    27301 aggttttggg ccttttggta gatcgataat gcctcctcgt gccttcccat ctcgtaatat
    27361 atgtctccca ttgcttccat gatggatgtg tcctgaggcg acgcttccag ggcatttgaa
    27421 tatgctgcga tcgcctcttc atgcttcccc atgttttttg cccgcctggc cctctcaagc
    27481 aactccatct cttccatgaa tgtgaaggtt cacctcccgc tatttaatgg atttcccatg
    27541 agccggcaat atgaaaaatc atgcattcac gctccacttc gactttggaa ttttatatgt
    27601 agagatatca tggatacgat cttcagaaag atttatacgt aacaagttta tattctcaca
    27661 atacaaggtg gcattcagag gcagtgcgta cgaggagctt caggctagac ttcaagcctg
    27721 gcagtttcga ctctcacatc ctccgagtgt acactccggg atggtttcgt atgcagaatt
    27781 ctcctctctc cagggtaaaa atcccgcttc tggctgccct gctcataatt actgcaggac
    27841 ttgcccctgt gagcgccgat caactccagg cgaatttcac cgcttcgcct ctctctggtc
    27901 cgcatccgct caatgtacag ttcaacgaca catcatctgg ccttgtaacc tcatggctct
    27961 ggtactttgg tgacaacaca tccaccaacc tgcaggatcc gcttcaccag tataccagcc
    28021 ccgggaatta cacggtcagc ctcaccgtcg caaacagcac cgggagccac agcctgctga
    28081 aggagggcta tatcctggtg accaacaccc cacctgttgc cgggttcagc ggcacaccac
    28141 tcaccggtca tgtcccgctg cctgtgcagt tcagcgacat gtccacaggt gtgattacaa
    28201 gttacctgtg ggattttggc gatgggaaca gttccactga ttcggaccca tatcacattt
    28261 atggcgcacc cggttcctac aacgtgagcc tcacggtcga aaatgacggg ggaagcaata
    28321 ccaccgacgc tgaacattat atcacggtca ctaatgcgcc tcccgtttca tctttctcag
    28381 ccaatatcac cacaggggtt gtcccactcc cggtactctt taccgatgag tcctctggcg
    28441 agggtatcac caactggaca tggacattcg gtgatggcgg aacatctgca gtcgcaagcc
    28501 ccgggtacgt atactcaacg ccggggacat attcagttac gctgaccgtc cagaacgacg
    28561 gggggaccaa caccagcacc agaaccaact acatcagggt attcaacgaa gtccccattg
    28621 tagatttcat ggctgttccg acatcgggcg gagagccgct caccgtccag ttccatgata
    28681 cgtcgcttgg gaatccaacc acctggcaat ggcaattcgg tgatggtggg atctcaccac
    28741 tacagagccc gtcacatacc tatcaaaacc cgggtaatta cacggtgacc ctcactgcca
    28801 gtacatccgg cgggaccagt acgacaacaa aggtggacta catccaggtc ctgcagcatt
    28861 atcctgtggc tgatttctcc gccgaaccaa cactcggagc gataccgctt gccgtcaact
    28921 tcacagacct ctccaccggt acgccgacct cctggtcctg ggactttggg gacgggaata
    28981 cgtcaaacga ccagaacccg gcgcatacct atgaatctgc cggaatctat accgtgaacc
    29041 ttacggtctc gaatggctat ggttctgact ccatagagaa agaggagtat atttcgaccg
    29101 gagaaacccc gcaggctgac ttcaatgcca ctagtacgga gatttataca aatcaataca
    29161 tcgagttcaa tgacctttcg accggcagtc ccgattccta cctatgggat ttcggggacg
    29221 ggtcgaacag cacatatcaa tatgcatggc actggtatcc agagcccggg aactatacca
    29281 tctccctgac ggtgtcaaac gagttcggca gcgataccga gacaaaggcg gactatattt
    29341 ctgtcacagc acacccaccg gaggctgatt tccagggatg gccacgggac ggatcattcc
    29401 ccctcactgt caccttcaac cagtggtcgt acggcggcta ctacgagaac ctctcttacc
    29461 tgtggaattt tggggatggc gagacatcga caagcagtga ctacgaactc atccacacgt
    29521 acatggaagc aggagtgtac aacgtgagcc tcaccgtctc tgcaaatggc ctgtctgata
    29581 ccgaagaaag ggtcaattac attggcaact ggtcaccgcc tccaccagtt gcaggattta
    29641 ctgcaacgcc gagagacggg tttgcaccct tggccgtcag tttcatagac cagtcaaccg
    29701 gctccccacc tctctccttt gcatgggact tcggggatgg cgagagctcg accgaacaaa
    29761 accctgtaca tacctttgct ggtgaaggca cttacaacgt gagcctcaca accaccaatg
    29821 caggtggaag cagcacggaa atgaaagagg ggtacatcat tgtcgggggc atcaccccgc
    29881 tcaaggctga cttcacctcc aacaaaacaa gtgggattgc gccgctctct gttcagttcc
    29941 tcgacgcttc agatggaaat cccagttcat ggttctggag cttcgagagc ggatattatt
    30001 atgtccttga tggcgcagtc gagaccagcg ctcttcccta caatcccggc cagatatcat
    30061 ccgagagaaa tcccgtaatg gtttaccagt cccctggtaa ctattccgtc atgctcaccg
    30121 tgagcaggac cggggagagc gacacaatcg aaaaggtcga ctatatacag gtgtcgccgc
    30181 cgcctcccgt cgcagcgttc aatccatatc cgtcgaatgg agatgcacct ctcgaagtgg
    30241 aattcgatgg atacatcccg tggtggtatt attacgacga atgggtgtgg gactttggcg
    30301 acgggacaac agaaacgaag gactatccct ggaccaccca tacctacacg gacccaggca
    30361 tctatgacgt aacactgaca gtcaacagcc agtacggaag caacacgacc acgataatcg
    30421 actgcatcaa tgtcacgcag ccttccgtgc ctcctgttcc caacttcgag gccactccgc
    30481 tgtccgggaa tgcgccactc gacgtcgctt tcaccgacac gtcgacgggc attgtcactt
    30541 cacgctggtg ggacttcggg gatggaacga ccgtgtggag caatgagagc gcggatatcg
    30601 agcacacgta tgccttcccc ggcacctaca ccgtctccct gatggcaggc aatgccgctg
    30661 gccaggagac actcacaaag acggggtata ttcacgtgtt ggcgtccgga ttacccccca
    30721 atgcattctt cacgatgagg ccgatgtccg gtgtcgctcc attgaccgtt tccttcaccg
    30781 atcgctcaat gggcgcgccg ctcgcatggg agtgggactt cggggatgga accacctcca
    30841 cggaacagaa ccctgtacac acattcacga cgaccggcac gttttccgtc accctgaaag
    30901 tcctcaacta tggcgggagc agttcccgct cggcctttgt ctttgtgagg acgatgacgc
    30961 ctgtttacaa gccgatcacc cccaacccgt cacagactgt gactgcgtct ccgaccgtca
    31021 ccccgaagga tgtcagcccc cccaggcccg gtatgacccc gatcgcattc tttgcggtga
    31081 gcaagagcat cggatttgcc ccgatgaccg tgcagttcac cgacatgtcc ttcaactccc
    31141 cgacctcctg ggactgggac ttcggtgacg gcgggacctc gaacataaag aacccgaccc
    31201 acacatatac cgctgcaggg acctataccg caatcctcac cgtggagaac acggctggga
    31261 gcagcaccag ctcgcgaaag atatatgtaa ggtaagtgag acccgtttcc tttctttttt
    31321 cgcgtcgacc acccctttct ccctttctgg aggtaaatgt ggtcaaacgc tccgcttatt
    31381 gatgagagta aaaaagcatt ctcgaaattc gagatctatt tcagaatgga aggattgacg
    31441 ggagactgaa tgctcctccc agcgcaccgg atggagcatg gaacaatccg gtcatggaga
    31501 ttacacgatc attgagaaat atgcagccag gtattgatga acgcttgaaa gggttaccag
    31561 gtggagttgg gagggtgcaa ctcatatggt gaacttcttt cgcgaatgat aacggtgaaa
    31621 ttggccaggg tttcaggaac cctctgcagt ctcatggatt tcagggaata ttgtcttgag
    31681 aaaggcaatt ccttccgcac cctggctcac cacagcctga agagcccgat cgaggtcttt
    31741 cactgagaaa tcgtgataaa acgggtagat ataccgtgac agtgtgaacc cgatcactct
    31801 tccgccttcc agtatccttg aaaaacccgt gagcggagag gtgaaagcct tggtcagctc
    31861 aaagatgaaa tgggggtctt tcttgtcgta ataggcattt aatgtatcct gttcctgtcc
    31921 tggcgggagc gttatctgga aggcgacaat aaagtaactt tttcccttat ggttctcgat
    31981 caaaattgga aaatctccaa acttcaccca aaatccacga aattcccctt cgtcaaattc
    32041 tttaacgatg gatacatcga tctcctgcaa atactgccgc agttcagcta ttgcctcatc
    32101 atcctctttc ctggcaaccg gtcctggcat atgtgagggt tcatcgcccg aacagtttag
    32161 tcttttcgcc agtaggtttt ccttatgatt caaacccctt ggttcaatta ttataaatac
    32221 cgcacggtca taactctccc ttggcgccag gaacgtgcgt gctcgccacc tgacgcccgg
    32281 gaaaggcagg ctggctatat ccgcacggaa tatagctttg aacgcgtgtt atttcccgaa
    32341 tgcaagatgt aggaggtgaa atcacattgg agtacctgaa ccggaccctc atactccctg
    32401 gcgtactcct ggtatgcatg ggaatcttct ccccggcagg agcatctctg gtcatagatc
    32461 tccaggaaaa ttccttctgt gacccgcagt tggagtaccc ccgcaacctg gacgatcttt
    32521 actccttatg caccctatcg tcccttcagg agggaggaga cttggccacg gtgcagggtg
    32581 tcacggacca ggttggttcc gtctccccgc aacgcccttt tgcacagggc actgtctctg
    32641 cctgggtgaa tcagaactcc atgcagggga acgcgaacag tgtgtcttcc atgatcgagt
    32701 atcaccagaa ggtgaccgca tcaggtaaga tagagaagtt tgtgttctct gtatcatgtg
    32761 tactctagca ggagagttgt cccccgacag gaaactccct caatactttt ttcccccaag
    32821 acctgcgcag cagaaatgtt gggtgatgac caattaggtg ctccaggtcg tatgctatcg
    32881 tataaaatca ccaatagtct tttggttccg gtcaaagtgt gaaagcgatg agagcctgag
    32941 accagcaagt ctgatcttct tctccgagag gaggggcatc accagttctc ttgcagccga
    33001 gactatcgcc cggaagtctc tcgtgtggca tggaagcgaa cgggccctgc tgaccgtgga
    33061 aaaatccgag aaccttatct ttaccgtgac agtcctggca aagatattcc gttctgcaag
    33121 ttcaccaacc agttggcgtg ccatgtcgtt taacgtctcc attaccagat ccaggttacc
    33181 tacatcggtt tcaaaggtaa tttccctgct cacagacttg cttgccgaat gctcccttac
    33241 ctcggactcg tcgatacccc gtgcaaggtt ctggagcggg actgcccatc ttccgaggat
    33301 ctccatgagc acctggatgt cggcccctgc caggtcgccg atggtgcaga ttcccttttg
    33361 atgaagggcc tctcctgtct ttcggccaac acccggaatc cggcctgcgg aaagggggtg
    33421 gatgatctcc tcgactctgc ccggcgggat gaccacaagg cccccgggct tctttaggtc
    33481 tgatcctatc tttgctatga cctttccagg ccctattccg atagaacagg tgaggcgctc
    33541 cctggtgagt atatcctcct tgatccgctc tgcaaccgct tctgcacgcg ggtaatctcc
    33601 caggtacgag agatccaggt aggcttcatc gatgctcacc tgctcgaatc gttctgcata
    33661 ccctcgcagt atagccatca ctccctctga agctttcgca taaagtggat aacgcggggg
    33721 caggaagact gcctgcgggc agagatgata ggcccgggag actggcatgg cagaacgaac
    33781 accaaatctc ctggcctcgt aggagcatgt gcagacaaca cctctcccgc ttccttcctt
    33841 tgggtccgca cctattacga cgggctttcc agcgagatcc ggatgctcgc gtatctccac
    33901 cgatgcatag aaactgtcca tgtccacatg gaggatgatc cggtgctccg tatcatgccg
    33961 gcgggcggcc cggctctcac gtgcatcgcc ctgaatcttg gtatccggag agagtggagg
    34021 cctgtcctct ttcattcctt ctgtgcatcc gcgttgagac tcacttcaga gtgggtgttc
    34081 atcgaggcaa gcctcctctc aagtaccttg ttctccgatt tcagcttcat gttctctctc
    34141 atcagcttgt tcagagtcat ggcaagggct gcgttctctt tcccgagtat ctcgatcctt
    34201 tgctcaagtt tctccgcgta cttctccaat tcatctcgat ccaaagagtg cgcccccgtc
    34261 atcacacatc tcactaatcc ggtcagatat agttttgcat gaggtgattc ctgccggcct
    34321 catacttctg ttcgggttaa aacggattcg tatacgattg aaccgcatgg tgcggcgggc
    34381 tcatatagcg aagtgcgccg gcaggatttc caagagcctg aggttccccg cttccctgat
    34441 tgaccctgcc ctgtaatttt tgtcaaatac cgcgacccaa tgcaatccca gcaagtatat
    34501 gtgatccccc tcatatttta ccctgagggc aggcagaatg aacgcgaacc tgcggtgcag
    34561 acctgcataa aaccaatatg agtcttctct catccaaacc accgttttgg gctcaattga
    34621 tgcttgtcat atttatcatc attcttgtcc tgggagtcgc cggaattgcc atcaactata
    34681 ccaccatggc ccgcgattac gaggagaatg aacagacact ccggaatatg actgaagcat
    34741 acttatcgtc atcattccac cgtatcgaca ccggcctgaa gatgtatgac aacacctaca
    34801 acgaccagat gagggaggga ttctcgatcg tgatggccga atataaccgg tccgggggtg
    34861 acccgtcccg gatgaacctg gagagactca aagaggctac tggcatgtcc gtgtatatta
    34921 tcaaccaatc gggtgtgatc gagtattcca cctccatacc ggatatcggc ctcgatttct
    34981 ccattatata tcctgacttc taccaatacc ttcagaggat atggcattca cccgggtttt
    35041 atcccgatcg gatcgtgacc gagtgggtga gcggtgcact gaccaagttc gcatacatgc
    35101 ccacgcacga ccaccgctac gtgatagaag tcggccttgc atccgaaagg ataactgcag
    35161 agaggaaggg tcttcgatac ggggacgtga gggaggaggc tcaggagttc aatccattca
    35221 tcgaggagat ccgcatttgg cagaagcaga aaagggtggt gggaaattcg agttaccagc
    35281 cggacgacac gcagtcagaa gtgctggaca caatactccg ggaccggaca agccacgagt
    35341 tccctgaccc ggatggaagc agggtaacga gatggctctt tgtggatatg ctggacccaa
    35401 agtatgccgc ggacatgagc ctgatcgtgg agattgtcta cagcaaagcc aaggttcagc
    35461 atgacctgca gctccttgtc ctcttccata tcctcgtggc gggtgtggtg ctcctcggag
    35521 gcggagggct tgcattcatc ctttcacgga ggctctcccg ccccatccag gggatagtcg
    35581 aggatgtgga caggatcgcg aagggggacc tcgaacacaa catttcccgc ccagcggcag
    35641 aggaattcga gcggattgcg cagtcaatca acgcgatggt ggagacgctc aaggggagca
    35701 tcacgcaact gcagcagagc gaagatgcac tcaaaaagag ccaggagaga taccggtccg
    35761 tggtggagac ccagaccgag atgatcgcca ggttccttcc cgacgggaca catgtcttca
    35821 cgaacgaggc ctattgtgca tatttcaacc tcccctgcac acagatcata ggtacccggt
    35881 tcaaaccatt gataccccct gatgagaggg caatgctcag ggaatacttc gcagccttca
    35941 cacccgatcg acctgtcggg accatcgagc atcgcataat acttcccggc ggcgaggtac
    36001 gttggcagca gtggaatgac agggcaatat tcggaccttc cggggggatc attgagttcc
    36061 agtcggtagg acgggatatc accgagaaga aactgatgga gagcgccctc agggagagcg
    36121 agacaaatta ccggaccctc gtcgagagcg ccaacagcat tatcctccgg ttcagagcgg
    36181 acgggaccat caccttcgca aacccgtacg cactctcgtt ctttggattc agaaaggata
    36241 gtcttgtcgg gaaaaacctg atggagacga ttgtccccga gttcgacagt gaaggagcaa
    36301 accttcggga gaagatcctc gacctcgctg ttcacccgga gcgctacagg acttcccaga
    36361 atgagaatgt cacccgcgaa cagaagcggg tatgggtctc gtggaccaac acacctcttt
    36421 ttgatgcaga agggaacgtg gaagagatcc tctcaatcgg aaacgacatc acgcgtataa
    36481 aagagatgga ggatgagctc cagcagttga acgagatgct cgaacagaaa gtcgttgaaa
    36541 ggacatcgga actcgaggac gccaaccggg agcttgaagc tttcagttat tccgtctctc
    36601 acgacctgcg ggctcccctc cgcgcaatag acggcttttc ctatatgatc atctcaaaat
    36661 attccagcca gttgcccggg gatgccatcc agtaccttga gcgcatccgc cataacatct
    36721 cctacatggg gcagctgatt gacgccatcc tcaacttttc ccgcatgtca aggcaacccc
    36781 tcatcaccca gacaatcttt ccctctgcga tggtgaaaga gatactggac gaactcctgg
    36841 cctccaaagg agagaggaaa gtcgagatca cgcagaacga tctcccccca ctgaccggcg
    36901 atcccgcact tgtcaggcag gtattctcca atctcctttc aaatgccctg aagttcacca
    36961 agatacgtaa aactgccaga attgaagtag gaagtacagt ccgtgagagt cgttctgtct
    37021 actacatccg tgacaacggg atcgggttcg acatgcagta cagggacaag atcttcgggg
    37081 tattccagag gcttcatccc gccatggaat acgagggtgc agggataggc ctgaccatcg
    37141 tgcagcggat catccagagg cacaggggga tcatatgggt ggagtcggaa gtcgacaagg
    37201 gaacaacctt ctatttcagt ttcgagccgg gccccaggga gtaaaaaacc aggggatctg
    37261 ctggccagcc tgaagagttc acaagaatag tcaatccttt ctctcctgcc gggggatcct
    37321 ggcaggcgat atcattgtat tgaatatcag ccataccata tgttgcatga acccgagtca
    37381 gcaaaccagt ctccaggtgc tcgatacgct ggatctcctc gtcaggcact ggaaccgggc
    37441 cgatatccct gcaatcctgt tgcatgcaga tccctcgtgc tcgggattcg gtccctggga
    37501 cagggagcac gtccaggggc acgatcattt ccgggcattt ctcgagagag atacaggccg
    37561 gcttcagggg cttggactga caggcctcca ggtcgaggcg atcgggacca tcgcgtgggt
    37621 ttccggggca ttccattcat cttctcgcga tggcactctg aaccctgaag gccgtttcac
    37681 ggcagttctg aaggggaccg gccatgcatg ggtgctggtc caccttcact tctcgttccc
    37741 gcatgcagac ctgggcgaaa agaataccag gtaagccggc tcccccttca cctgcctcta
    37801 tcagggtgag gggaactgct ggctcacgca gtggatggca cccattcccc ttatcatctc
    37861 gctgcagtcg attcccacca cgactcttcc cgggaagacc ttcctgagtg tcttgagggc
    37921 gcgcctgtcg gcagggtcat tgaacgtcgg aacaagaacc gttccgttcc cgatgtaaaa
    37981 attggtgtag ctggccggca cggagccttc tgattcgcct ggcatgggga gcggcaccac
    38041 ccggaggggc gatccgtcgt gcgcgagggt cctgcgcaga atctcgtaat tctctttgag
    38101 tattttgtag ttatcgctcc cgcgctcctt ctcaagggca cacacgacag tcctttcgtc
    38161 cacgaacctg gccacgtcat cgacatggcc atcggtgtcg tcgcctgcaa ttcccccctt
    38221 cagccagatg acacgggaga ctccaaggaa gtccatgagc acctgctcga tcccctccct
    38281 cgagagtgaa gggttacggt tggggtggag gaggcattgt tcagtggtga tcaggctgcc
    38341 gcgaccgtcg acatcgatag atcctccttc aagaaccatg tcgggggtga atacaggaat
    38401 atgcaatgac ctggcaagga ctgaagggat gatgtcatcc ccctcgagct ccgggtattt
    38461 tccaccccac gcattgaatc tccagttcac catagccatc gaagaatcgc gcgcatgcac
    38521 gaggaacgtg gggccgtaat ccctgaacca cacgtcgcag taagggtaaa cgaagaactt
    38581 cacatgccat ggttcgatgc cggcgcaaga gaggatctcc tccaccctgg atcgcatgtc
    38641 ctggtctctg accatcaggt ataccctttc tctccccctg agtgcctgga tcatttctaa
    38701 gtaggagtgc tcgacgcatg ggagatcgaa gaacgtgtcg ttgtcgtgcg gccaagaaag
    38761 ccaaatcccc tggtggggct cccattccgc aggcatgcga aaaccagagg gcgctcgcgc
    38821 ctccgctcca ggaaatgtgc cggtgtccac cagagggcca taggtgtcag gacgccggtt
    38881 actgaagaat ccccatcctt cccggacgag gtcgttttgc gagaggtcta tctctgcgac
    38941 aataatctcc tcccccctgc ctgcctttgc gataatattc ccgaacgcat cgcagacaaa
    39001 ggacccgcca aagaacgtga tctccccttc aatccccacc ctgttcactg ccgccaggtg
    39061 gaccgcgttg gcaatcgcgt gacctctctg taccgtctgc caggcatcct cccagtctcc
    39121 ctccaggggg tcttccattc catggacggt gccaatcgcg gtggggtaaa agatcacctg
    39181 cgcccccatc agcgaaaccg tcctcgccgc ttcggggaac cactggtcgt agcatatgag
    39241 gaccgcgata cggccaaaat gggtctcctc cacgacgtag gatgaccctg gtgagaaata
    39301 ctccttctcg tagtaagacg gatcgtacgg aatgtgcact ttccggtata catcccccgt
    39361 tgaaccgtca ggccggatga tgactgccgc gttgtagtgg cggccacccg cgcccttctc
    39421 gaggagaggt gcgacgatta gcccctggtt ccgccgcgca atctctccaa gcaccctcgt
    39481 tgtctccccg gggatatgtt ccgcgtaccg atccgcgtcg gcaccatgga ccctcgggaa
    39541 atagggtgcg gagaagagct cctgcaggca gacgacctgc gctccgcggt cgaacgcgtc
    39601 ctgtaccatc cctgaaacct tctcaaggtt ccctgcgtgg tcaggcccgg cggaagcctg
    39661 gaccagcccg atccgtatgc gcgatgatac cataggcgag ttcatgtagg cgtttttcct
    39721 atttcatccc caccatacgt acaggacaag ccgttgccgc agatatcatc ggaaacattg
    39781 ccagaaaggt gcaagattcg ccctgagcgc ggcagcatgc tcctttacca ctgcatgtgc
    39841 cgagggatcc cctgcccttg tcagccattc cccgtaacct tccggctcgc ggatattccg
    39901 aaggtcgagg cccgccatcg cggcaatgat cccaaggttt gtgtatggaa ggcctccttc
    39961 gacgctgtac ccgccttcga ggaccgcgac cagcctccct ccacagagat tttcggcgag
    40021 gtcaactgct tttttcatca ggtctgcata tcccctcgcg gtgagggcaa gtccggtaag
    40081 gggatcagtg aaatggttgt cctgtcccgc agagatggcg atcaggtccg gtgagaactc
    40141 ttctgccagc ggcgcgatca cggtgtcaaa gagaaaatgg taactctcgt cccctgtccc
    40201 tgcggggacc ggcatgttcg cggtgaatcc cgcgcccttc cctgaccctg tctcttccac
    40261 ggctcccgat ccaggataga atggccactg gtggactgaa gtgaagagca cggtcggatc
    40321 ttcgtagaag atctgctggg tgccatcgcc gtgatgtgca tcccagtcca ggatcagcac
    40381 ctttttgact ccccgtgcct ggacatgccg caccatgatg gccacgttgt tcaggtagca
    40441 gaaccctgca ccggtccccg ggcgcgcgtg gtgtccaggg ggcctcacga gggcaaacgc
    40501 gttcctcgcc tcccctgaaa ggaccgcatc ggcagcccgg agcgcacccc cggctgcaag
    40561 cagggcatga gagaagaggc ctggagggac attcgtatca aaatcgatga acccgcctct
    40621 tctgctcgcc tgctccagga accgcaagta ctcggggata tgcacgcggc cgactgcctc
    40681 catgggagct tcggatggcg tgaacacctc cacagacgga tgatcgaaca gcccctcctc
    40741 gcgaagctgg tcgagtgtgt acgaaagcct ctcctttcgc tctgggtggt cccgggactg
    40801 ctcgtgcagg aggtattttg ggtgaaagac gagcgcggtt ttcatggggc ctccgccgta
    40861 atacccggcg cgatctggac caccacgtct gcgatccgcg cactgcggaa cgagcttctc
    40921 accacgtcgt acgcgtggaa atggagcacc cgcacatcct cgacatcacg gggatcggcg
    40981 cctgcggcaa tcgcccgctg tttgacctcc gcccggcacc gatcaagcac tgcctcgtcg
    41041 tccccaagcg ttctcaactc ctggggttca ccattgaaga ccacggcacc cctgccggag
    41101 tcgaacctcg catggagggt gagagtcacg cgggagaccg cgacccctac ggcattcgca
    41161 cactctgcat gaggcggtat ggtgaaggac gcgccggaat gccgcgcgat gcgggggacg
    41221 aggaactgtg caaggtaacc agtaccaaca accatccggg cgccacttgc ggcaacggcc
    41281 cttgacacga gggagatata ctcctccagt gcacgttcgg cggacgtccg atcgagtagt
    41341 cccgatgccc cggcatttcc tatctcgcat cctgtcacgt tcagcgcatc cgtcagagtg
    41401 ggccgaatcc ccccaaatgc aagggcgttt cccatccgcc ggggctgtat tgtctcctct
    41461 atcaccgagt ccccgccata agggagcgtt atcgccgcga cagcacgcgt caccgtccgt
    41521 tcgccatcgg ctacgattgt ctccatcgcg ggctttcccc ctgaaagtct gaccaggtcc
    41581 gtggtggtgc ccccgatatc cactaccagg cactcctcga ccttgctcag gtaaaatgcg
    41641 ccaagtgcgg ccgctgccgt gctcgaatgg tagaggacgg aagggtccct gattgcatag
    41701 gctggtggaa tgagcccccc atcgcccctg aagaagaaaa agtccttcct ttcagcccca
    41761 atctctcctg caaggaggca gaccgcctct ttcatgcgtg cattcagcct cgtggttgcc
    41821 gccctccccg ggaagtcaag ggtggcaagg tgatggctta cggcaatccg ctcctcggca
    41881 tagtggtcca gggcacgggc cgccacctcc tgctcgagcg aaggatttcg caccgagaac
    41941 tttccggtaa ttgcaaggac atcgccacgg gaggaggcaa gaactccgcc gacctccgtg
    42001 gagtcgaggt gctccacgat atcgccccgg tggttcaccg cgcccctgat acctccgggc
    42061 cagaagagac ctggcccggg tatcaggatc gtctgaaccc tggctggatc accggtgagc
    42121 acccggttga gcggagtcga ggtgcttacc gccaggcggc cgggcgccct ggcgtgtgac
    42181 agcacctccc ggatgccgag tgtgtttgga accttgacgg atgtgacctc gcggtcaacc
    42241 atggcgagat ccgtgttggt cccgccggca tccatcccga taagcatctt ttgatgaccc
    42301 ccttcaccac acgttcatcc tggtaccaaa aaaaaatctc tggtatcctt ttcgggcacg
    42361 tgaacagatg gggagtgcca gggtcccgcc ggcatcacca atcggcgccc cggaatggcg
    42421 tgatgagtgc ccttgcacaa ggactgttca ccgggccctg cgggggtacc cgatactcat
    42481 gaggctctct gccacctcgc caaggaccgc ctggtcctct atcgtcgggg ggatagaata
    42541 gcgttcgccg tccgcaatct ttttcatcac gccccggagt atcttccctg aacgtgtctt
    42601 gggaaggcgc ctgacaacga ccgccgcctt gaacgatgcg atgggaccga tgcgatccct
    42661 gaccatctgg atcagctcct ggacgacatc gccgttctca cgccgcaccc cggacttgag
    42721 tacaacgaac cccaccggga tctccccctt cacctcgtcc gccacgccgg tgaccgcaca
    42781 ctccgccacg tccgggtgcg atgccagcac ctcttccatc gcaccggtag agagccggtg
    42841 ccctgcggtg ttgatgatgt catcggtcct gcccatgatc cagagatacc tgtcctcgtc
    42901 aatgaacccg gcatcgcctg tcagatagaa tcccgggtat gccgagaagt atgtccggac
    42961 gcactcgtcg tccttctgcc agagcgtggt aaaacagccg gggggaagcg gcaggcggat
    43021 cacgatgttt cccgtgaccc ccacgggggt ctcgattccc ctgtcatcga ggacacgaac
    43081 atcgaagcct ggcatcgccc gggtgcacga gccgggcttg acaggaagca tggtaagtcc
    43141 gacagggttg gccccgattg cccatccagt ctcagtctgc caccagtgat cgatgacggg
    43201 tatcgagagg cgcgctttcg cccaggcaag ggtgtcaggg tcgcaccgct ccccggcgag
    43261 gaagagggtt ctcatccggg acaggtcgtg cctctcgagg tattttcccc ccgggtcttc
    43321 ccgcttgatg gcgcgaagcg cagtcggtgc gcagaagagc acgttcactc catgctggga
    43381 cgcgacgcgc cagaaagccc cgggatccgg tgttcccacg gattttccct catagaggat
    43441 gctcgtgctc ccataggcga ggggtccata gacaatatag gagtggccca ccacccatcc
    43501 gacatcagat gccgcccaga agacctctcc cggcgacagg ccgtagaggt atctcatgct
    43561 ccagaggagc gctgcgagat gaccgccgtt gtccctcacg actccttttg ggatacctgt
    43621 cgtcccagat gtatagagga tatagagagg atcggttgcc tgaacgggaa cgcattctgc
    43681 cggcgacgcg gtgtccatga accccttcca ctctctgtcc ctcccgtctg cgaggtttgt
    43741 ggggcactcg ggtcgctgta cgatcacgca gtgtgcgaca gggattcctg caatggctat
    43801 tgcttcgtcg atgagcggtt tgtaggggat gacacggctt acttcgatcc cgcaggacgc
    43861 gataagcact actttcgggc gggcatcgcg gatcctggat gcgagttctt ttggggcaaa
    43921 cccaccaaag accaccgagt ggatagcgcc gatccttgcg caggcgagca tggagatgac
    43981 tgcctccggg atcatgggca tgtagatgac cacccggtcc cccttcacca ccccgaggtc
    44041 ccggagcccg cctgcacacc tcgcaacgag atcctgcagc tccttgaacg tgtatctcct
    44101 tatggtcgca gtgacagggc tgtcgtacac gagggcgacc cggtcacctc ttccttcctc
    44161 aacgtgccgg tcgagggcat tgtggcaggt gttgaggatg cctcccggaa accacctggc
    44221 aaatggcagc ccggatgtat ccagcacttt ttcccaccgc cggacccatg agatctcttc
    44281 cgcagcctct gcccagaaac gctccgggtc acggagcgac cgggtataca catcgtcata
    44341 cccggcctga tgaataccag accctccctg ccatgctata acatgacaaa tctttctccc
    44401 tgccggtcaa atacctttcc cgcagggttg tccgtttctc tgacacggcg ggaattggag
    44461 gatcagtcct ttatagaggg gattcgccat ctctttatca tcttcacgcc aatgtgtaat
    44521 ggcagatgga gattccgctg gtcatcctca tcggtatatc gcttgcaatg gactgcttcg
    44581 ctgtctcgct ggccgcgtcg gccgcatgtc cctcacgaag gatcaggatc gccctggcat
    44641 tcggcatctc ttttggagtg ttccagtccg ggatgatgat cctggggtgg tccttgggta
    44701 ctgccatcgt tgcccttgtt tccggagttg cccgctggat tgcctttggg atactctccc
    44761 tcatcggggg gaagatgatc tgggaggggg tatgggaaag agaggcagga tggaacgctg
    44821 actgtctctc ccccgcaacc attctcatcc tcgcgacggc caccagtatt gactccctcg
    44881 gagtcggtct gtcactggga tttgtggata gtgcaatata ccttattaca tttgtagtag
    44941 gtgcggtggc attcatcttc tcctgcctcg gtgctgtgct tggaggagcc cttgcagaga
    45001 agttcggcaa gaaattcgaa gtcctgggtg gaatcattct tattgggttt ggagtgtgga
    45061 tcctgctcgg acatcttgca gccccctgat tggcctttct gcaggaagaa tcttttcagt
    45121 acctcctctt cgctttctcc tgctttttcg cacgaatttc actctcgtga aaataaaatt
    45181 gcagatctcc cgccaatcct tgagaatatt tatcactccg atcctctcac ctattgagca
    45241 acagaggtct ggtgaagggg atgccggata tttaccaaat catataccag aaacttgaaa
    45301 agatgggagt cctcgaggtc cgccagtacg ccgtgatcga gatgcctccc tatgttccgc
    45361 tctgcataga ccggctctcg gaggatgtct atgccctgtc ccagaacccg gaggtggagg
    45421 gggtgatggt cgctgacccc gacatggaaa tacgagtgga ccatgcaaga aagacagccg
    45481 aaccgcttac cttgcagtcc ggtgaaacaa ggcgggtggt gtacaccgct ccgggcaggg
    45541 ttgacttaaa gacgcggatc gaactctcaa ggtttctgga tacatggctc tcggacctct
    45601 tagacaaagg attcatccgt caccagtaga aggtgtccgc tcttgtccct gtgctggaga
    45661 tcgcagtcag gatgagggag atcattcatc ccgttgtgtt tcagagacag tgtgcatatt
    45721 tcccattttt gccaatacca taactgctcc gggcaggcag aaggacccgg gatcctgaca
    45781 tgcctattct cctgacagag ccgccttttt atcatcccgc atttgaggag gcccaccagt
    45841 acatcaccgg gtgttaccgc ataccaaaaa gacctctggc aatattcatc ccctgcgcat
    45901 tgcgaaaacc ctacagccag agcccaagcc acaggctctt caggcggatg atcagcgatg
    45961 tatttgacga ggaagactac cacctggtga tcttcggcac ctgcgggaca gtccctgctg
    46021 aactggaact catgtacccc tttgcccatt accagtatat gctcgggaag tgcgacgatc
    46081 ccaggattcg agacgatttc ctggagatag agacatcccg gctcgagagg tatctccgga
    46141 aaacaacaca ccactacatg aggcgctgtg catattgcct gggagtgttc cgcgaggcaa
    46201 tgatccacgc ctccgagcgg agcggcgtgc cgctcgatct tctgcttccc tcgaatcaga
    46261 ccatagaaac catgcgggat ccggactgcc ccttccctga cggctcgctc tccatgaagg
    46321 aatacatgga tgagttcagg aacggactca tctcgatgaa agaatgatcg tcccgttgtt
    46381 ttcccccaaa aaacgcacca tcattcattc caggttcact ttaaataaaa aacacccgca
    46441 gtcctgtgca ggcatgtact gggatatggt cgatcaccac ggtaccatgt tttttaaata
    46501 cttctccgtt tctttcagga acatgcccca tttctcacca gagatgtcct tttttttcta
    46561 aataagcgca tttcggggcc ctgatgattc caaaaggttt aattatgatt gctctccatg
    46621 taatatcagg tgtattagtg tccaactaag tcaccgactg aacgttccaa tccatggtgg
    46681 aaaccctgcc ctgaaaggca gggtggacgc ctcctgttga tgccacca
//
LOCUS       DAOQYR010000007        44689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_946_length_44689_cov_8.954922, whole genome shotgun sequence.
ACCESSION   DAOQYR010000007 DAOQYR010000000
VERSION     DAOQYR010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 44689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44689
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_946_length_44689_cov_8.954922"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PKM87_01645"
     CDS             complement(<1..629)
                     /locus_tag="PKM87_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNQ28798.1"
                     /translation="MPTCAACGADMKEGATFCPSCGMKQGQDASREEALFWTAKARVI
                     SSRPVVTQLIMVFGVGFLFVMVLLLILDVESAIMLAPYVIATAVFIIVLGLIIAAAIQ
                     FFTKGGLTTDFAVTPEGIGYRAGKESKAINRATLAGTAIGGSLAGAGGSMINISREMD
                     FMSWAEVKSVTIYPRERSLVFYRKALIFPFALYCTPENFEQVKTLVKKYA"
     gene            837..1337
                     /locus_tag="PKM87_01650"
     CDS             837..1337
                     /locus_tag="PKM87_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNQ28799.1"
                     /translation="MPQVHIIRMPFCTSCGQQLADTDRFCTSCGAPMEPADKPAPSPA
                     DTKEIRWTNSVLGMTSDFFVNLEGAGQEASKKTSTVNTGIAVAGGLLGSLSAAGGGMI
                     VKSRENDFIRWNETRSVALNRKKKTVTVTRKSLIFPIRLYCTEENYEQVAAFIRQHVN
                     PVLIKE"
     gene            1546..2364
                     /locus_tag="PKM87_01655"
     CDS             1546..2364
                     /locus_tag="PKM87_01655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNQ28800.1"
                     /translation="MGMSGLLVLAIAPAIFILCYFYLRDRYEPEPLGWVVKVFLVGAI
                     MVIPAALIETPFPSGLLTAVFVAPIVEESLKFAAVYFTVYRDEEFDEPVDGIVYASAA
                     ALGFAMLENVMYVMEGGVAVGIVRAVVSVPAHALFSSIWGYSLGIARFRHEKDRLFLI
                     AIGLAGAMAFHGIFNLMASDSELLGLIVILVLLVPGGWWLAHRHIGHAHAHPASAQFL
                     TGKRAPPKESPGIPGAGIPRERGSLSSGYCTKCGNPLSPDTKFCGMCGEPVERV"
     gene            complement(2401..2901)
                     /locus_tag="PKM87_01660"
     CDS             complement(2401..2901)
                     /locus_tag="PKM87_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ28801.1"
                     /translation="MHNPLSALVLVLLLLSAGCITTAEEWNQRGETHHTMGQYDEAVA
                     AFDRAIALDPDSHHAWKNRGLSLALLGRDEESRASFSRAIALCPNCSDAYYYQALARN
                     ATGDHRGAMESLDHAIAIAPQNQDQVVTLFQSLIMRGDLLTVENRLEEANLSYRIANE
                     VMMGTI"
     gene            3007..3447
                     /locus_tag="PKM87_01665"
     CDS             3007..3447
                     /locus_tag="PKM87_01665"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HNQ28802.1"
                     /translation="MAQFCESCGARIKEGDKFCEQCGAIVPGPAGVPQAQGAPGEVAH
                     PPKNPTLALILSFFFSGLGQIYNGDTLKGVAIYFGTLIGALLFIVPGIIVWIYGVYDA
                     YTTAKKMNEGTVPYKKTNTLFMIGFVVMVLVIGGIVLIMSLALV"
     gene            complement(3467..4042)
                     /locus_tag="PKM87_01670"
     CDS             complement(3467..4042)
                     /locus_tag="PKM87_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNQ28803.1"
                     /translation="MPLFCKWCGAQISPDSRFCEECGHAKDPTTDVDSGCQAIPLLEL
                     HEGLLGAGRALAVMEVFPGELRIYRLPDYFTAEVEDLRNRLDNAWLQNVENLRDGKEA
                     VVSWDWCSEPLLSAVAECRSDLVQGLIVMRTEEIISVVIEQIQSDTAWDLMTISSTSK
                     KCIFDLTGPVAWHAVKVLRAVLGERVRYEEG"
     gene            4841..5428
                     /locus_tag="PKM87_01675"
     CDS             4841..5428
                     /locus_tag="PKM87_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNQ28804.1"
                     /translation="MPFCPDCGARLEAGSKYCTDCGARVPVDLDSGPVFDAAIESTGA
                     ETVVAIIPNLMRVRGLGILLKGPVWQHLVLTSQRIIVVQRSRKGLDRLKQDIGIIGPD
                     YEFIFLKTMKPERILDENPDSKVIPLIELVALEVLKFSSYSTEDGIEPYWQVQITTKK
                     GILKLRTDYHDDPGEYFQNPALKRLLGERLVLQEM"
     assembly_gap    5631..5730
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5731..6078
                     /locus_tag="PKM87_01680"
     CDS             <5731..6078
                     /locus_tag="PKM87_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28805.1"
                     /translation="AVEADPSWMAAWYLKAYSLAMLNRTNEACAAVDQALLLDPFDRD
                     SNNLKADILESLGRDEEAARFRDVAAGGGSISQPDVPIMPPPTTKSPLNPLVSVSTLL
                     VVACLIGIRAGGR"
     gene            6109..6825
                     /locus_tag="PKM87_01685"
     CDS             6109..6825
                     /locus_tag="PKM87_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28806.1"
                     /translation="MQNHEWKWAYLAAGGAVFFILLIAFANQAMDGMSGGYAVMFVSF
                     FLAVTLVAVSLLFWSRARTMDAILEGRNLLAHWEYSAEDSLASAGREFFEYREANRAL
                     FMVVGGFFLIAIVLLLIFGGEAGVLTSLVLIGAVGIIALVAWGAPRLVYSRAIRASPE
                     AFIASNGVIYREAVYPFRSFLNRLEGVSYQDTTPDTQALLIFSFIQVVGLYILRPYEV
                     RIPVPGGEESHARDIARVLG"
     gene            complement(7071..7742)
                     /locus_tag="PKM87_01690"
     CDS             complement(7071..7742)
                     /locus_tag="PKM87_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNQ28807.1"
                     /translation="MEILSAWWMAFLAGLYAPLGSPCVIPLYPGFLSFLAGRKGERER
                     STLTLGMIVVAGVILSMLVFGIIFVAVVQVSLSRFLSFLSPLAFLVLAAFSIVLILDI
                     DLSRWTGSITQPRSGRPFLDAFLLGLFFGVIILPCNAAAVVALLAIGTTATGFLANLG
                     SFFAFGLGMSLPLLAFAAVSGTRSLEILAWFSRHRRGIHVVAGVVMLGVSLYYLLFVF
                     SPLCG"
     gene            complement(7746..8303)
                     /locus_tag="PKM87_01695"
     CDS             complement(7746..8303)
                     /locus_tag="PKM87_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNQ28808.1"
                     /translation="MNVLKFSPVLPPAGLVLATCCIIFSAGCMGTTGHVNGGTMTGEN
                     WRNIELRDVQTGETFSVASLSAKPLILYTFTVSCPICTLQQKEISTLKKELGEDVAVI
                     GIDIDPKEEEAVLKSHIEKNGFLGYYTLSPPGMAGSLTERFGPVVVTPASAPVILVCP
                     GGDARLLGTGIKTSAQLSSLIRAVC"
     gene            complement(8329..9030)
                     /gene="radB"
                     /locus_tag="PKM87_01700"
     CDS             complement(8329..9030)
                     /gene="radB"
                     /locus_tag="PKM87_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadB"
                     /protein_id="HNQ28809.1"
                     /translation="MNPPPLSTGSVDLDEILGGGLARGVITQVFGEPASGKSTICVMA
                     SVSALRSGDQVLFLDSEGFSAERFRQIAGEDAEKLAAGLYLYEPEDFTQQGVMVKSLD
                     AMLRGGGTGLVVVDSATGLYRSQLERGRDAMQKLTGQVLMLLGYARRYRIPVLISNQV
                     YTDTTRNVFVGLGGTALEHLSKTIIRLERRDHLRRATLAKHRSLPPGAWFDFEITQNG
                     IRAWEPPHNPKKEGI"
     gene            complement(9027..10214)
                     /gene="larC"
                     /locus_tag="PKM87_01705"
     CDS             complement(9027..10214)
                     /gene="larC"
                     /locus_tag="PKM87_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HNQ28810.1"
                     /translation="MRVLVFDPFRGAAGDMICGALLHLGAGADLVSRAMESVVAEPSI
                     KEVTRAGIRAVKVDTRAPVLLRSLTEVLERVNGADAPAGAIGMACRVFERIAAGEAVI
                     HGECPHFHEVGADDAIADVVGACTALSSLGVDAVAVGTIAVGGGTVRTAHGTVPVPAP
                     ATLAILQGTGLAVQYGTGEDGELCTPTGAALLAEFATAFPGVPPRGRVVATGYGAGER
                     NPPSIPNVLRATLIETSHFSGGDEVDILETNIDDVTGEILAGCVDRLMSVGARDVCAV
                     PCTMKKGRPGYLVRVVCFPGDSARLSVALAEETGTLGVRCIPSIHRFVAERETRIVTI
                     DLSGRRWDVPVKCGTLGGKAFSVKAESDRVFEVARECSVPAREVARLAEAEAWKAAGR
                     MSE"
     gene            10254..12674
                     /locus_tag="PKM87_01710"
     CDS             10254..12674
                     /locus_tag="PKM87_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HNQ28811.1"
                     /translation="MPELHLKVDSAYPGDQGGGKARLDPETMLFLKISPGDIVVVEGK
                     RRTVAKVWRSLVEDWNQNKIRIDNFTRMNAGVSIGDTVRISRVQDEIEAKRVVLAPPE
                     DLPRNVPINITPHIVNALIDFPVVKNDLVPLSSGLPFLQTQFVPFKVVEIEPEQAVII
                     TKNTHIEFSEKPAPGVEGVKRISYEDIGGLKDELQRVREMIELPMRHPELFQKLGIEP
                     PKGVLLFGPPGTGKTLIAKAVASESGAHFISIAGPEVISKYYGESEQKLREIFDEAEQ
                     NAPSIIFIDELDSITPRREEVTGEVERRVVAQLLTMMDGLEERGQVVVIGATNRVDAI
                     DPALRRPGRFDREIEIGVPTEPDRIEILKIHTRGMPLSEEVNLEQLAQQTHGFVGADL
                     AALAREAGIRALRRYLPEIDLDEEEIPEEVLDTMEVTAGDFRDALRDVSPSAMREVML
                     EVSHVTWQDVGGLSEAKQEVREAVELPLTDRERFEELGIRPPRGVLLYGPPGTGKTLI
                     AKAVANESGANFIAIRGPQLLSKWVGESERAVREIFKKARQVAPAIIFFDELDALAPT
                     RGGEVGTHVMESVLNQILTEIDGLEELKDVVVLGATNQPMLVDPALLRAGRFDRLVYI
                     GEPGMDDRARILAIHLRGMPVEGSAMEALLGSLEGFDEGSIDELGEALGREKTVSPGE
                     FARIAKTLKVPEKSPLSRSQRRRYLSELMISRSLKVPDEALEELVRDIAARTEGYVGS
                     DLEALCREAGVFAMREGSRQVAKRHFDESLAKVHPTMNPRLTEYYERIRQSFKGGLPK
                     EVQPPEYQ"
     gene            12738..14279
                     /locus_tag="PKM87_01715"
     CDS             12738..14279
                     /locus_tag="PKM87_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNQ28812.1"
                     /translation="MQNLSAFLDNHALDPSACAIVWPATGERCTYQDLLSRVQRLASA
                     LSGRGISKGDRVCIYLDSCPEYLVSYFAIWRTGAVAVPANIVLKEGELRYLLQDSGAR
                     GIICSSKGYPVARTAVSSCPSLNLVVCTGDAPEGTVPWDLVMQEEPSTGSAAPCGFDD
                     PCQLQYTSGTTGRPKGAILTQGNWMAALEAEREVLGLRPGDRYLGIYPMAHVGVSWGI
                     SVLRAKGTYVIMERFEEEEYLRLAREHRITVLSAMPPVIHGLCDAPKEAEEALGTVRV
                     IISGGGQLLPAVWEAFDRRYRIPIANSYGLSETIVVGTGTATVPGLPELTRDYRSVGI
                     PIGYSEVRIVDSSDPSVVLGPGETGEIALRGPSVAIGYWNMPRETAETFLPGGWFLTG
                     DMGHLDEGGVLSITDRKKDMIVMSGWKVYPTEVENILLQHPDIADAAVFGTPDERKGE
                     IPVAAVVAREGCRLDGEDVSAFCRSRMAGYKVPRRVIFVESLPRVHGWKLLRRTLREE
                     FSNTS"
     gene            14337..14921
                     /locus_tag="PKM87_01720"
     CDS             14337..14921
                     /locus_tag="PKM87_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HNQ28813.1"
                     /translation="MAKDNRRSTGISGLDLAMEGGFPLGTSVVVQGSPLTGIDHMARQ
                     FWGSDSSSGSYLMIDGEVEEGMVPAAGLTIDQLAEQCRGDKIVVDSLSSIIITSGIDT
                     AISFLGRAKKDVAARNANIMLLLYPDLHPSHEEIRIMRMADVVIELKEVIFMNEIERQ
                     LAVHKIRRGQVPKRLIPFNITDKGIELSTTSRVV"
     assembly_gap    15043..15142
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15260..15359
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15360..16228)
                     /locus_tag="PKM87_01725"
     CDS             complement(<15360..16228)
                     /locus_tag="PKM87_01725"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNQ28814.1"
                     /translation="MDPFARLPILPTTVVGSFPVERGSGLGGLLDPFRHAVKVAVDAQ
                     VRAGIDIISDGQVRGDMIQAFSSRLPGIRGQDVVNKVLPASGPITVNDTRYALSCHPM
                     VKGILTGPTTLSCGLHIATPVYRDRQDLALDLAAALVPEALALERAGACMVQVDEPIL
                     STGAADLYAGEEALSRVTSTLQVPVSLHVCGDLSGIVDRLLGFPIAMIDIECAKSPEN
                     LELFDRNALNGKRLGVGCVDSSDTAVEPVSVIRERIEHAVDLLGPEFLLIDPDCGLRM
                     LPKDVAFAKLSHMV"
     gene            complement(16235..17527)
                     /gene="aspS"
                     /locus_tag="PKM87_01730"
     CDS             complement(16235..17527)
                     /gene="aspS"
                     /locus_tag="PKM87_01730"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050560 - aspartate-tRNA(Asn) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HNQ28815.1"
                     /translation="MRIPIQEVTPGVERAEIVGFVHELRDLGGLSFLLVRDRTGIIQV
                     TVPKKKVPEAVLSAVRLVSRESVVRVAGTVKAIDKAPGGRELVPETFEIVSLAESPLP
                     LDVVEKVPAELDTRLDSRFLDARRPRVAAVFQVRNACMRAIYEHLFSSGFIQITTPKV
                     VAAATEGGTELFPIAYFEKEAFLNQSPQLYKQMMMGAGFEKVFEIGPIFRAEEHNTTK
                     HLNEATSIDVEVSYADHHQVMALLEEIVIAAYRRVDSECRPFLELLDLPDFEVPSSAF
                     PRLPYEEAITIAQKKTDEPITYGDDIGTAAEKAIGEEMGCHYFIVDWPTAIKPYYAMP
                     CENDPSVCRAFDLMHPRMELSSGSERVHQHDLLVRQIQSKGLNPDSFAFYLTPFRYGM
                     PPHSGWGLGAERLVMTMLGLQNIREAVLFPRDRHRLVP"
     gene            complement(17644..18624)
                     /locus_tag="PKM87_01735"
     CDS             complement(17644..18624)
                     /locus_tag="PKM87_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28816.1"
                     /translation="MTQSYNLSPVLRELLEFAETSLGTEIQLVRRTDVPPQGVLIDDF
                     TFGTGKHVIAFSSSQLGMLKDYTICRHCLELLAKGCAAQHNEYRVISFSKDCALPACR
                     QVYLDILKDEGTRNLAVWRKKQLVFLLYMLFHEAFSDLPLTLLANIVIARRYPVIRNA
                     QVYFLLKESMRDMHDLVPVKEFLPQRFFVLHNGMYYARDMLLAYVLSEYKLNPVINIP
                     ELQRFRNLDVKEMMSHRWSRSPWYHTKMVGDALSNILKLTVTMDMERDLDAGYFQELF
                     ALSREMLSRWWVMMGMQDWYVWESPGHLKAAVAAQAGMEEAIRQEIFGTE"
     gene            complement(18744..19394)
                     /locus_tag="PKM87_01740"
     CDS             complement(18744..19394)
                     /locus_tag="PKM87_01740"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNQ28817.1"
                     /translation="MPPQERPSTREEVILVLNEKFAVMRRLYGVKKIGLFGSVARGDE
                     RPESDVDIEVEFESGMDTYANYIGLSLYLDELLVRRVDLVMSRVLASYLRPELGAEFA
                     ALSRDKVYLGQILDEIAYLSHRTKGVSAKDFQKDETLRRAVTRSMEIIGEAAARVSGA
                     CRQDSRGVSWNELEGLRTRLIHPYFSPDWGLVWNVLNVYLPTVEPAIRKLHARLAR"
     gene            19534..20043
                     /locus_tag="PKM87_01745"
     CDS             19534..20043
                     /locus_tag="PKM87_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28818.1"
                     /translation="MHIPEILALVMITVLLPAAFSGCLMQPGTELGEVPTPPPAGLPS
                     PHGTAVGVEVEVAAGPANMTLTFLPGGEFWAGDRILLAGTTNLSPGNHLLVEVIPVSF
                     GPTRKGEQVAVSGSSGVVEVVAGKNGLPNAWSFTIDTRGWEPDEYLVRVQGIEVPASL
                     LTSRFTLRP"
     gene            complement(20062..21324)
                     /locus_tag="PKM87_01750"
     CDS             complement(20062..21324)
                     /locus_tag="PKM87_01750"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNQ28819.1"
                     /translation="MSIFRSLKGALGGNSAHQMDEVEREDVESVRRALAYGDVEARWN
                     AIRAAGELGDAFIDPLVNALKDEHWIVRRGAGETLARIGLPAVPPLIEMLGSEDESVR
                     QEAMRACACAGEPAVDSLIRALSRENPAIRRGAAEILGEMQVQAATGPLIETLKDSDP
                     GVRREAAAALKLLGSEEAIAPLISLLGDESGYVRISAAEALCSLGGRSIDPLVAALAH
                     PQPEIRQRAGLALASIGTLAVEPLIAALSHGDSLVRQGAAGVLGRIGDPRAIPGLIRV
                     LGDKERKVRQEAVRALAALGYPAITPLVAAFREGDVVVRNCTMEALWLIGGPATRPLL
                     ELLSDQNSEVRRRTALLLGEIGDAAAVDGLTRLLSDQVPSVRREGFEALEKIRKKQGE
                     GSLGTGPVPRQPDQDPEKQGEGKKPGSW"
     gene            complement(21405..22307)
                     /gene="cofD"
                     /locus_tag="PKM87_01755"
     CDS             complement(21405..22307)
                     /gene="cofD"
                     /locus_tag="PKM87_01755"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HNQ28820.1"
                     /translation="MITFLSGGTGTPKLVRGMRRHVPDHEISVVVNTAEDLWMSGNFL
                     SPDIDTLIYLFSGLLNTTSWWGQEGDTFTTHDELTRLDSGEYVAIGDRDRAIHILRGE
                     LLRRGMNLTRATAHLCASLKVRAQVLPMTNTQVETIIMTGGREIHFQEYWVRHRGTLP
                     VDEVKRRWETPPAATPEVLSAINSAEAVIIGPSNPITSILPILECGGVVEALESVPVV
                     AVSPFIGDAPVSGPAAALMRARGFAPDSAATRDLYARFLDFFVQDTRDTVGVPGATRM
                     DTLMNREGVDESLAAGILSLVRGT"
     gene            22359..23564
                     /locus_tag="PKM87_01760"
     CDS             22359..23564
                     /locus_tag="PKM87_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNQ28821.1"
                     /translation="MKIIKDPVHGYVEVPSSMVSLLDSAPMQRLRYVRQLGFSYLVYP
                     GAHHTRFEHSLGTMHLAGVMARQLDLDADERLLVAAAGLLHDIGHGPFSHAIEPLSEE
                     FAAKSHQDVEGLLEQPETGGILERMGVDPDGVTSMIKGEHPLSGIIHGELDVDRMDYL
                     LRDAHYTGAPYGTVDAHRLIRSTTLADGRVVLEEAGINAAESLLIARTLMRPAVYFHH
                     VSRIAEMMFQGAVMAHVESNAKSNLLSLLYDDDAACMQALLTSPSPVARDLSRRIYQR
                     RLYKRAIYVNRNQVNAARVAQCSPYPKRAALAAKIAAVAGLSPHEVLVDIPPFPGDMS
                     LHVQVRNRHRAVGISELSPLLHTLNETRRGQWRLGVYTLPEHRDEVAGIATDVLHVRP
                     ETMQERLFG"
     gene            23645..24088
                     /locus_tag="PKM87_01765"
     CDS             23645..24088
                     /locus_tag="PKM87_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28822.1"
                     /translation="MIEYWIVVTTGVAAVAIVLCGAYLYNEIKSREFVQNRVKLKVYV
                     ALLQAVTEINVAGNDAYALDRAKRNLASTLNQINLIAGPQVLLHVNELLDFLNETREG
                     DYDVLKEKNILNSIVKAARRELDPASARALEESQFRFRFYNPPRP"
     gene            24208..25455
                     /locus_tag="PKM87_01770"
     CDS             24208..25455
                     /locus_tag="PKM87_01770"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HNQ28823.1"
                     /translation="MREFIEQMRERSLVVDVDERPRGEFDAPKMASTTDSLLFFHDLP
                     GGRAIMNVTATRKALSLALGLGEREIGMKLSKASYDGSVVREGSLSMRPADLSRIPAM
                     KFFPKDAGKYLTAGIVFSGYGGVENASIHRMLVLDQERVAARLVEGRHTYTLHREALA
                     RGEELPVAVAIGVHPAVTFASCSRVPKGKELPFAAELLGGEVRVHQCSNGVLVPEAEI
                     VLEGYIGSDTAREGPFVDITGTYDSVRVQPVIRFTGMHTKNDCIYHSILPGGNEHRIL
                     MGVPYEPIIFRAVSAVTEARNVVLTTGGCGYFHAVVQIKKSTQGDAKNAIMAAFAAHT
                     SLKHVVVVDEDIDPSDPAEVEFALATRVRGDRDILVVTGARGSSLDPCRVGDGTNVKV
                     GVDATMVLGQEAEFLRVGWQEGQ"
     gene            25452..26633
                     /locus_tag="PKM87_01775"
     CDS             25452..26633
                     /locus_tag="PKM87_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HNQ28824.1"
                     /translation="MILSAEEEAILAGEEGETRARMLEILLALGKVYGAERLIPIRSA
                     QVSGASYKTIGRFGLDWLQQLDTKVSVPTVLNPVGMDRSRWREMGITPEFARVQEQVL
                     DAYRRLGVTMECTCTPYYLYMTRFGDHLAWSESSAVAYANSVIGARTNREGGPSALAA
                     AIIGKTPEYGLHLVGARKPQVVIRVEEGDRLVDAAHFGALGYLAGRLVGSRIPLLEGI
                     RPKKDLLKALGAAMAASGAVALFHVKGITPEARVFSYPTEGLEVIEIGYGEISALMKE
                     IPVDAVALGCPHCSPEELSRIAGLLAGKKVGIPVFVFAARDVAVHHPAEVAAIEESGA
                     HVFADTCMVVSPAMERFGAIMVNSGKAFAYIPNMCGAAVRLGSTEECIEVATRPAAGA
                     S"
     gene            26718..27959
                     /locus_tag="PKM87_01780"
     CDS             26718..27959
                     /locus_tag="PKM87_01780"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNQ28825.1"
                     /translation="MAFSDLHLNTMDRYKPAILLLLVAVSVILEAVVHHAMGIEVVYS
                     HFFYIPVVLGAIWYGKKGVLVAVFLAAVYAGDYYLINGVVHTDAVMRALMFVVVAFVI
                     GMVMDYIHREQEQVMTQMANAALGGGTSGGFRGNLGELKAKLRSSVNVKRLKEEGDIG
                     GLIGALGHRDAAIQYEAAEALGVLKEPSAVTPLMTALTGDRYSGVRWKAAEALARIGG
                     PAVEPLVGALGHSDEDVRWKAAIALGEIGDARAIPPLVALLEDQDRFVQSRAAYSLGQ
                     FGNAALSPLLLVLETGSPAARRGAAIALGKVGDPAVMEPLLSALSDPDEGVRSAVIET
                     LAGLGEGVHSRVLAHIRGLSPGERDRVFQSLREATNPRFIEGFLPVLSMADEDAKAVI
                     LSLVRQGGSGEQGGKDPAPGG"
     gene            28029..28739
                     /locus_tag="PKM87_01785"
     CDS             28029..28739
                     /locus_tag="PKM87_01785"
                     /inference="COORDINATES: protein motif:HMM:NF016166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF432 domain-containing protein"
                     /protein_id="HNQ28826.1"
                     /translation="MFGIYQIPFQTDAPGITVSVESGLATSTYRRTSPEGTLAKRLGY
                     REGRVIINPVEPINLPTEVTGMLEFHFMPVTMRPNSRITICLKFPVEIGVFVGADDNY
                     TLLDVFSLARPKYSLYGTPEKGVITRHVECEVYEDAPPTNPLEEGVLFLTLRNSGRDW
                     IEVTRAVFESSALRLFYDKTVAMAGEMEVFSRMVAETKVIDRPPEEGMKQALRVYPTK
                     KSLIPERPPFMMEYGVGD"
     gene            28742..29596
                     /locus_tag="PKM87_01790"
     CDS             28742..29596
                     /locus_tag="PKM87_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNQ28827.1"
                     /translation="MAFESLRMELFGNVTLLDLIVFAIAVFVALVIAKIVAIYLKKSL
                     SDRIPPAEMDFLIKVIQAAIVIIGVYLALPSFNINLADMLVVGGTMGLVIAFASQRIV
                     SNFGSGLFLIAERPMKPGDNVNIGTVSGTVEEIRVLSTIIKTHEGVYVRVPNEKIFTS
                     EITNFVANVARRFEYTIGISYQSDAEHAIRIVKTLLEDHPFVLRHPSPSVFVDTLADS
                     SVNLRVFIWAPSRVWWSVRTEMLWAIKKALENEGIEIPFPQQVVTFAPGTGLSLNPGC
                     GGEGEGHG"
     gene            29589..30104
                     /locus_tag="PKM87_01795"
     CDS             29589..30104
                     /locus_tag="PKM87_01795"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNQ28828.1"
                     /translation="MGDPVLEGLTDTLVNGTMAERREAVRRLAGRGEGAIQPLLALLR
                     QPVGNDARWYAAVALVKIGPAAIGPLIEAVAGNDDQDFRRYVAAALGHLGEPAVAPLI
                     EAMRDADPKMRGFLAQALCRIGGPAIPAVRSMLGSDDPVVRECATLTLWQMGEEGIAS
                     LLSSVDEESLQ"
     gene            30216..34076
                     /locus_tag="PKM87_01800"
     CDS             30216..34076
                     /locus_tag="PKM87_01800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II large subunit"
                     /protein_id="HNQ28829.1"
                     /translation="MMQCSPGMERYLAGLQEGLEAAMAVAEKARAAGIDPRTTVEIPV
                     ASDLADRVEALLGIKGLAKRIREMEGEMSREEVALHIGEDFVQRRFGEETDEEILDHA
                     IRTAMAVLTEGVVAAPTEGIAKVGIGRNDDGSRYLKIYYAGPIRSAGGTAQALSVLVG
                     DFVRRALGMNRYVPRQEEVERYIEEIRQYNSIMNLQYLPSEREIRLIVQNCPVCIDGE
                     GTEQEEVSGYRNLERVETNTVRGGMALVLAEGLALKAPKVQKNVRALSLDGWDWLDEL
                     IGGAAKSGDGDDAAGIKPKDKYLRDLIGGRPVFSYPMRKGGFRLRYGRSRNTGFAAAG
                     LNPATMHILGDFLAVGTQMKIERPGKAAGIVPVDSIQGPTVRLKSGDVLRVDDAVQAR
                     ECAPEVDYILDIGEILIGYGEFLENNHPLVPPAYCHEWWCLEGGAGRPGSEEEAIALC
                     FEGAFLHPDFTWLWDDLSCTDIAELADFVSQTGELRHGILVLPPDERFKGMLEEILIP
                     HRVREGLVCISDTYVFLACLGLDLKLKKRPAWQNAPMDDPLSLVSHLSGFGMRSRGGT
                     RIGGRMGRPGKSRPRKMNPPPHSLFPLGEAGGSRRSFQEASNHAPQPNMDGGVITIET
                     GRRRCPSCGAETFRNRCECGAHTEPVYSCPRCKREQAGPGCPSCHTALACSQVVTLNV
                     KQEYAAAMERLGLRESSIALVKGVKGLISRERTVEPMEKGILRAARDLFVFKDGTIRF
                     DMIDLPLTHIRPGEVGVLPERLRELGYAEDIAGEPLLSPSQVVELKAQDILVSDSCAE
                     YMVKVAQFLDELLEKCYGLPPFYRVQTRDDLLGHLVIGLAPHTSAGVLARIVGFSRAN
                     VGYAHPFFHAAKRRNCFFGDTEIETFDGREWCTRPIRQIVAENFDLSRPGIDRLGTYY
                     SDPSSTLLVRTVDREGKPHLRRVTSVSLHRSPEALIRFETRGGREIAVTPDHAMLVWD
                     ICSLRKIRAVELKEGDPVPVMVGANVLTDHIIRREVVPSPDTRVFCLTVAEDHTVLAN
                     GIFTGQCDGDEDCIMLLLDGLINFSRSFLPETRGGSMDAPLVLTTRIDPAEIDKESHN
                     LDVGPGYPLELYLATLNYAHPKEVEGLVDRVGRRLGTPAQIEGFSFTHDTTDISAGPL
                     ESTYTQLKSMIEKLDAELALAGKIRAVDEDDVAERVLTTHFIRDLQGNLSAFSKQKFR
                     CVKCNTSYRRMPLAGKCNRCGGNIIPTVHEGSVKKYLEMSRDICRKYKVSEYTRQRVQ
                     VLDMAIESTFGQEKSQQMGLADFM"
     gene            34091..35023
                     /locus_tag="PKM87_01805"
     CDS             34091..35023
                     /locus_tag="PKM87_01805"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ28830.1"
                     /translation="MSSGSGQVTTGMDALSTSGLLDILKHHTPSGHGSIQAIRKDFSM
                     LYCSFQHDRHAHQQPILLSGTIPACWISALQSRPDRTVLFFHGGGFSLGSTADHLGLC
                     TRIAEEAEARVISIDYRLAPEHPFPIAIEDCINAYLSLIGKGIPAKRIILAGISAGGT
                     LALSTLLRARERDLPLPAAAVCMSPAVDMNFRGNSIYTNQETDWVTPGRLEYIREVYL
                     NGHNPSDPLASPIFADLSGLPPLYIQAGGNEVLIDGIQAFVARAEDAKIEVQFDCYDG
                     MFHCWQVFADVVQEGVVAITRIGEYIQSRLIQNS"
     gene            35183..36349
                     /locus_tag="PKM87_01810"
     CDS             35183..36349
                     /locus_tag="PKM87_01810"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNQ28831.1"
                     /translation="MYDLSRLRQEFPVLSRCTYLDSASTSQTPKRAVEAMCAYFYEYA
                     ANHGRGSHRLARRTTEEYEQAREALGGFLGADPGHLVFAKNATDAINMVAHGTCWEPG
                     DEVVTTALEHHANLLPWMALQGRGVRVKVLPQRDGMVDPADLQEALTPRTRLVAVTHV
                     TNVLGSVQPVEEMASIAHDAGVRILLDAAQSAGHMPLSVGGFDFVAIPGHKGLLGPQG
                     TGALFVADYSDLSVTCHGGGIVSEVSLSGFTLLPPPARFEPGTPNIPGVIGLGAAIGL
                     VEELGVGEIHHHETSLGIQAHEGLSSIEGVAVYSPRGSPVVSFNIPGMSPDDCAAALD
                     RRKGICVRSGLHCAHPLASSLHPLGTVRASMGCYTTGEEVAMLIETVEEIARSR"
     gene            complement(36385..37266)
                     /locus_tag="PKM87_01815"
     CDS             complement(36385..37266)
                     /locus_tag="PKM87_01815"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNQ28832.1"
                     /translation="MKRREVFTLLLLLAILAGGLWSAGCVSPDIPGALSSQKASEGAM
                     GVDYNYAPYSTPSPAPVAGAWAGDEQKIIRTARIDLEVRDVMATRDALQSIAAENEGY
                     VGSLSLERYRGDRLSGTLVMRVPAASFEKALATVAALGTLQSQSVQAEDVTEEYVDLQ
                     ARRAALANQLVQYQRIMEKAENVSEILEVQEQIERVQVEIDRIDGRLKYLDSRIDYAT
                     ITVSFREPEPVGAGGGPSITSVINEGIAGFLGVAAALVIILISIIPLVVLGLVAYAAY
                     RWWKGRKGTRPEEKKPE"
     gene            37417..37499
                     /locus_tag="PKM87_01820"
     tRNA            37417..37499
                     /locus_tag="PKM87_01820"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37451..37453,aa:Leu,seq:caa)
     gene            37636..38886
                     /gene="hisD"
                     /locus_tag="PKM87_01825"
     CDS             37636..38886
                     /gene="hisD"
                     /locus_tag="PKM87_01825"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNQ28833.1"
                     /translation="MFNALDVDRWVAERRSSLDEAKESVAAIIQAVRKGGDGALLAMA
                     RKHEPDITSVRVTEEEIAAAYDEVEDRLVESLVEAEARITRFHELQKERSLWLEEVEP
                     GIVLGVKTTPLDRVGLYVPGRRAAYPSTALMNAVPAKVAGVPEMCACTPPPVLPLTLV
                     ALDIAGVGEVYRIGGAQAVAAMALGTETIRPVQKIVGPGNVYVTAAKMMLREYAEIDF
                     PAGPSEIGIIADGTANPRFIAIDILAQAEHDPDAACVLVTTEKTLADRVRAEIGRLLE
                     KAPRREIIDKALGHSGYVIVPTMEEAVSGMNEIAPEHLSIQVADALTVLNAVRHAGSI
                     FVGPYTPVAAGDYASGTNHVLPTAGYAKTYSGLNVSHFCKTSTVQIIDREGLDAIGDV
                     IEAIADAEGLNAHAESVRIRRSFN"
     gene            complement(39090..39956)
                     /locus_tag="PKM87_01830"
     CDS             complement(39090..39956)
                     /locus_tag="PKM87_01830"
                     /inference="COORDINATES: protein motif:HMM:NF025091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HNQ28834.1"
                     /translation="MRAPTRLRELIAADPILVIPVVHDPLCARIAEQAGFSAIFSAGY
                     ANSAALLGRPDVGLLTLTEMVDCASRIVDATTIPVFADGDTGYGNVTNVVRTVSLYEK
                     AGVGGVFIEDQVSPKRCGHMEGKALIPAEEMAAKVRAAVDAKVDPDLVIMARTDAIAV
                     NGIEDAIERANLYYEAGADLTFVEAPESVEQMRRICREVNGPAFANMLPGGKTPVLSA
                     PELDEIGYAVVADPVSCTYAITHAVRHVFSELKRTGSSASYPGHLVGFDEFNRLVGLQ
                     EIREREQSYYRK"
     gene            40095..41387
                     /gene="rbcL"
                     /locus_tag="PKM87_01835"
     CDS             40095..41387
                     /gene="rbcL"
                     /locus_tag="PKM87_01835"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III ribulose-bisphosphate carboxylase"
                     /protein_id="HNQ28835.1"
                     /translation="MAIDWYMDFVDKGYEPAKDELDALFYFEPAAGISSEEAAGRIAS
                     ESSTGTWTTLHALPPRMRDLQATVYEIDGRYVRIAYPQGLWEEGNAAQLLSGIAGNVF
                     GMKAIKNLRLIDVSLPQGYIRHFKGPHFGMEGIRKMMRIEGRPLTGAVPKPKVGFSAR
                     EHAEIGYETWMGGFDFVKDDENLTSTSFNRFEDRVREMTRMRDRAEQATGEEKSAFLN
                     ITGETETMKERAKMLADSGWNYAMIDVVVAGTAAVSTLRDYCSDLGLAIHAHRAMHAS
                     FDRNPKHGITMQFLAKMMRLIGVSQIHTGTAVGKLTGSKKEARILAEVLRERRVKEVR
                     HALLSQEWGPIKSAFPVSSGGLHPGLVPDVLDIYGTDLVLLVSGGIHGHPKGTRAGAI
                     AAMKAIEAWEEGMSLDEKAKSSPELAQSLEKWGYYRPK"
     gene            41520..42065
                     /locus_tag="PKM87_01840"
     CDS             41520..42065
                     /locus_tag="PKM87_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28836.1"
                     /translation="MRPEYREIFSPHLSLWQFLAALGFGILAALTLFSDLAIPVGHGL
                     LAEPSEVFVTLGAAFSGPFGGIIVGLLQGVVYAPERNIPAHMLAGFLWGAWYVVLYRW
                     SLGKGRWTRAALWAASIPVYYYVLLLPLHIAIASFTLGIPLLPLYAEAAGGVLPEMAF
                     TLLVTSAVMAALPERWAAPLD"
     gene            complement(42068..43246)
                     /locus_tag="PKM87_01845"
     CDS             complement(42068..43246)
                     /locus_tag="PKM87_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNQ28837.1"
                     /translation="MQYRFAGRMHCAPPSFLGKLFAVSSDPRIISFAGGLPSSAFIDV
                     EGLADASRRVFEEEGREALQYSTTDGYLPLREFISRRYRQRLGLDIPASEIQVVNGSQ
                     QCLDLVAKIFLDRGDPVAMERPGYLGAIEAFSLYEPTFYDVAFGAEGPDIDEFSRVIR
                     EVRPKFFYGIPNSQNPSGQTYSAERRKAVAEVLEGSETVFYEDDAFGELFFDGRPRKP
                     VKALAPDCCILSGSFSKVIAPGMRIGWICAPKEILAQFNCAKQAADLHSNFLCQKVLS
                     RYLETCDLDDHVRRISAVYGQNCRLMCDLLDDLFSPGMYHTEPEGGMFLLATLPKGLD
                     SMKVFEEGLKGGVAVLPGTPFYVSGGGDSTIRLNFSSMDGERIRVGMERLARVIRALV
                     "
     gene            43421..43606
                     /locus_tag="PKM87_01850"
     CDS             43421..43606
                     /locus_tag="PKM87_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28838.1"
                     /translation="MVKTTIALSPETRDLIRDLGNKGETYDGIIIRLLKDAGWKHLDT
                     RWNEILENDEFIPLDEL"
     gene            complement(44018..44422)
                     /locus_tag="PKM87_01855"
     CDS             complement(44018..44422)
                     /locus_tag="PKM87_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNQ28839.1"
                     /translation="MTGTRADADTVGAIVKDLYSHDWSIRQAAVRRIGSLASAEDLSI
                     LFSRLRIEKWYIREAAAAGIRALDCPNLIPVLRSVMRDGDDILRNAATCALGNMRSRE
                     AEAILEKAAEDPDCRVRRNAKVSLQRIRAPLP"
BASE COUNT         8732 a        13319 c        13807 g         8531 t
ORIGIN      
        1 gcgtattttt tcacgagtgt tttgacctgc tcgaaatttt cgggggtgca gtagagggcg
       61 aacgggaaaa taagcgcttt cctgtagaag acgagggacc gctcccgggg atatatggtg
      121 acgctcttca cttcggccca gctcatgaag tccatctcgc gacttatatt gatcatgctc
      181 ccccctgctc ctgccagcga acctccgatc gcggtccctg caagagttgc gcggttgatg
      241 gctttagact cctttccggc ccggtagccg ataccttccg gggtgacggc gaaatccgtt
      301 gtgaggcccc ccttcgtgaa gaactggatg gcagcagcga tgatcaggcc gagaacgatg
      361 atgaacactg ccgttgcgat gacataaggc gcgagcatta tcgcagactc cacatcgagg
      421 atgaggagga gaaccatgac gaagaggaac ccgaccccga agaccatgat cagctgggtt
      481 accactggcc ggctggagat gacccgggct tttgctgtcc agaacagggc ttcctcccga
      541 cttgcgtcct gtccctgttt catcccgcat gatgggcaga aggttgcccc ttccttcatg
      601 tctgctccgc acgctgcaca ggttggcatt ggatcgctcc tttatccagt atgtgttttc
      661 cataaggtaa aaaaagggca catatatcca gggaaaggag gttccctccg taagatcctg
      721 tacctgtccc tcatccattg cgaggttcca actgcgggaa gggcaggaaa cgagcaggct
      781 ggctctactc caagcgtccc cccgggatgt aacctttgca tgccaaatca tttttcatgc
      841 cacaggtcca catcatccgc atgccgttct gcacctcctg cggacagcag cttgctgaca
      901 ccgaccgctt ctgcacctcc tgcggggctc ccatggagcc agcggataag cctgccccat
      961 cacccgccga tacgaaagag atccggtgga ccaacagtgt cctcggcatg acctcagact
     1021 tcttcgtgaa tcttgaaggg gcggggcagg aagcatcgaa aaagaccagt actgtcaata
     1081 caggaatagc cgttgcaggc ggcctccttg ggagcctgtc cgcggcaggg ggcgggatga
     1141 ttgtcaagag cagggagaac gatttcatca ggtggaatga gacacggagc gttgccctca
     1201 accggaagaa gaaaacggtg acggtaaccc gaaaatccct aatcttccct atcaggctct
     1261 attgcacaga ggaaaactac gagcaggtcg ccgcgttcat caggcagcac gtgaaccctg
     1321 ttctcatcaa ggaataacag ggtgaggaga ccaatccccc caacacgcga tgtgcactgc
     1381 agtccagcca tatcaggatg cgaggggtaa tgctactctc ggttttttta ttcacctgcc
     1441 gttccgggga tacctgaaaa cagcgcattc gtgcagttgc ggtccaggga tcatatcagg
     1501 ccgttccgtt tcgcagcggt tatcacgtgc cggaaaatac cggtaatggg aatgtccggc
     1561 ctccttgtcc ttgccattgc gccggcgata tttatcctct gctacttcta cttgcgggac
     1621 cgctacgagc cagaacctct cggctgggta gtgaaggtct tcctggtcgg cgccatcatg
     1681 gtgataccag ccgctctgat cgagaccccg ttcccctccg gcctcctcac cgcggtcttc
     1741 gtcgctccca ttgttgagga gtcactcaag ttcgcagcgg tctatttcac cgtgtatcgt
     1801 gacgaggaat ttgatgagcc ggtggacggg attgtctacg cctcggctgc agccctcggg
     1861 tttgctatgc ttgagaacgt gatgtacgta atggagggcg gggttgccgt tggaatcgtc
     1921 cgggccgtgg tctcggtccc tgcccatgcc cttttttcct caatctgggg ctacagcctc
     1981 ggtatcgccc ggttcaggca tgagaaggac cggctgttcc tgattgccat tggccttgcc
     2041 ggggcgatgg ccttccacgg cattttcaac ctcatggcat cggattccga gctgcttggc
     2101 ctgatcgtga tcctcgtcct gctggtcccg ggagggtggt ggctggccca tcggcacatc
     2161 ggccatgccc atgcgcatcc ggcatcggcg caatttctca ccgggaaacg agcgccaccc
     2221 aaggaatccc ccggaatacc gggagcaggt attccccggg agcggggctc cctttcaagc
     2281 ggttattgta ccaaatgtgg aaatccactc tcacctgaca caaagttttg cgggatgtgc
     2341 ggggaaccag tggagcgggt ctgaacgtac gtcccgggct acctgcgccc ctgcaggcct
     2401 tcagatagtg cccatcatga cctcgttggc aatccggtag gagagatttg cctcttccag
     2461 ccggttttcc acagtgagaa ggtcgcccct cattatcagc gactggaaga gcgtgacgac
     2521 ctggtcctgg ttctgcggcg caatggcgat ggcatggtct aagctctcca ttgcaccccg
     2581 atggtcaccg gttgcattcc gcgcaagagc ctggtagtaa tatgcatcgc tgcaattggg
     2641 gcagagtgcg atggcacggg aaaacgatgc cctggactct tcatccctcc caaggagcgc
     2701 aagcgacagt cccctgttct tccatgcgtg gtgcgagtca ggatcgagcg caatggcccg
     2761 atcgaaggct gccactgcct cgtcgtattg ccccattgtg tggtgggtct ccccccgctg
     2821 gttccactct tccgcggtgg taatgcaccc ggcagagagg aggaggagca cgagcacgag
     2881 agcggagagt gggttgtgca taccctcatt ctgcgattcg gggttcaatc aaggtttcgg
     2941 caaggagact gtgcggcatt cggggtcgaa aaaaacctat ttattcccgc atttcgattg
     3001 ggtaccatgg cacaattttg tgagtcatgt ggagcccgga tcaaggaggg ggacaagttc
     3061 tgcgaacagt gcggggcgat cgttcccggt cctgcaggtg tgccgcaggc gcaaggagca
     3121 cccggagagg tcgcacaccc ccccaagaac ccaaccctgg cccttatcct ctcatttttc
     3181 ttctctggcc tggggcagat ctacaacggc gataccctga aaggcgtagc gatatacttc
     3241 gggacgttga tcggggccct cctcttcatc gtaccgggaa taattgtctg gatatacggc
     3301 gtctatgatg cctataccac ggcaaagaaa atgaatgagg ggacagtgcc ttataagaaa
     3361 accaacactc tgttcatgat cgggttcgtg gtgatggtcc tggtgattgg cggaatcgtg
     3421 cttatcatga gcctggcgct ggtgtaggac atggccactt ttttctctac ccctcttcgt
     3481 atctgacccg ctccccgagc acggcccgta gaactttgac ggcgtgccag gcaaccgggc
     3541 cggtgagatc gaagatacac tttttactcg tggatgagat ggtcatcagg tcccaggcag
     3601 tgtcggactg gatctgctcg ataacaaccg agattatctc ctcggtcctc ataactatga
     3661 gcccctgaac caaatcactg cgacactctg caaccgcgga aaggagcggc tcggaacacc
     3721 agtcccacga cacgacagcc tctttcccat ccctcagatt ttccacgttc tgcagccagg
     3781 cattgtcgag cctgtttctc agatcctcaa cctcggctgt aaaatagtca ggaagccggt
     3841 atatacgcag ttctcccggg aaaacctcca tcacggcaag ggcccttcct gcgccaagca
     3901 gaccttcgtg gagctcgaga agcgggatag cctgacagcc ggagtccaca tccgtggtcg
     3961 gatctttcgc atgtccgcac tcctcgcaga accgtgagtc gggggagatc tgtgcaccgc
     4021 accacttaca gaataaaggc atgggaattc ctttccagcg gtagggtgct caaggctcat
     4081 aaagagagga gatatcgtcc tggagtgccg ggcggttagc cgaaggagta tgcatttgtc
     4141 atctttgctc acttctcggt atactccacc aatgacaatt tcgaccaaca tgccgattta
     4201 ccggcttgtt tgtttcaggt gtcggagttt cgggcctctt tcctgccagt ccagaattcg
     4261 ccggggcagc actccgacac gtcatcctgt tttcctggct ctattcgcca tcacaaatcc
     4321 atctttactt ccaatggtcg ggaagacatg gtcacttttt cattgtaagt cttccatccc
     4381 agtggcgggg acttcctttt cgctatcacc ttcgagtttt tcttcctgat gttttccctc
     4441 tctttcatgg tattggagat ttatttcagc atgagtaact cactccccga cagggaaccc
     4501 gatctccaat gcgggaaaag gcatgccgtg gcttggttcg gccttacatt tgcaatgaga
     4561 ggaattggta tctaaggcct gcgagtggtg gtcctttaac tgatccctgg acgcggggtt
     4621 tttccttgcc ccctgcccaa tgggggatat tttcctctct tttctcgttc cactcgcggc
     4681 cggacactcc tgccatcact cgatcggcaa ttaaccgggg aggggtgggg gcttatattg
     4741 aaccgttctt tgccataccg ggagatgcgt gtcccgggat gatgcagaaa gattatggtg
     4801 caattggaga ataccccgat aagaggaggg agaccacagc gtgccattct gcccggactg
     4861 cggggccagg ctcgaggcgg gatcgaagta ctgcaccgat tgcggggcgc gggtaccggt
     4921 ggatttggat agcggaccag tatttgacgc agccatagaa tcgaccgggg ctgagacggt
     4981 ggtcgcgatc ataccgaacc ttatgagggt ccggggcctg ggaatcctcc tgaaggggcc
     5041 ggtgtggcaa cacctcgtcc tgacatccca gcgaattatc gtcgtgcagc gcagcaggaa
     5101 ggggcttgat cgcttgaagc aggatatcgg catcatcggg ccagactatg aattcatttt
     5161 tttgaaaaca atgaaaccgg agcgaatcct tgacgagaat cccgacagca aagtaattcc
     5221 gctcatcgaa ctcgtcgccc tggaagtctt aaaattttcc agttacagca ctgaagatgg
     5281 catcgagcca tactggcagg tgcagatcac gacgaaaaaa gggatcctga aactcagaac
     5341 cgattatcac gatgatccag gtgaatattt ccagaatcca gccctcaaac gactcctcgg
     5401 agaaaggtta gtcctgcagg agatgtaatg gggcccatgt cgaaggggga aagggatcca
     5461 ttttgttacc tgcaactctc cctgcagccc tttctttgga aaagccataa gtcccgggaa
     5521 gtgattctgt ggcgactgcg gcaatacact gggaaaaatc cccttgtttg agtgttcccg
     5581 tccggagttg ctgtccgtct cccggaaatt aaattgatta ccttgcccgc nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcggttgagg cagatccttc gtggatggca
     5761 gcctggtatc tgaaagctta cagccttgct atgctgaaca ggacaaacga ggcatgtgca
     5821 gcagtcgacc aggcactcct cctcgatcct tttgatcggg actcaaacaa tctcaaggcg
     5881 gatattctcg aatcactcgg cagggacgaa gaggcagcca gattccggga tgtggcagcc
     5941 gggggaggat caatatctca gcctgatgtt ccaattatgc ccccaccgac cacgaaatcc
     6001 ccactaaacc cgctggtttc ggtctctact ctcctggttg tggcctgtct catcgggata
     6061 cgtgctggag gcaggtaaga gggggctttt tgcgcaggag agccaggaat gcagaatcat
     6121 gagtggaagt gggcatatct tgcggcagga ggagcggtat tcttcattct cctcattgcg
     6181 tttgcgaacc aggccatgga cggcatgtcc ggggggtatg cggtcatgtt cgtctcattc
     6241 tttcttgccg tcaccctggt tgcagtgtcc ctgctgttct ggagccgggc gaggacaatg
     6301 gacgcgatcc ttgaggggcg gaatctcctc gcccactggg agtattcggc agaagattca
     6361 ttggccagcg caggtcgtga gtttttcgag taccgggagg caaacagggc gctcttcatg
     6421 gttgtcgggg gttttttcct cattgcaatc gtccttcttc tcatctttgg tggcgaggcg
     6481 ggagttctca cttcccttgt tcttataggt gcagtcggta tcatcgccct ggtcgcatgg
     6541 ggtgccccgc gtttggtcta ttcccgggcg atccgggctt cacccgaggc attcatcgcg
     6601 agcaacgggg tcatataccg ggaggccgta taccccttcc gttcctttct gaaccggctt
     6661 gagggggtct cttaccagga taccacgcct gacacgcagg ccctgcttat cttttcgttc
     6721 atccaggtcg tgggcctcta catcctccgc ccatacgagg tcaggattcc ggtccctggc
     6781 ggggaggaga gccatgcgcg ggacatcgca cgggtgctcg gatgacaagg gattgaaatg
     6841 catactgcga atgcagttat acgaagcgct catgcacata caattgcaga ggcggatacg
     6901 atggcctcac ctacgatgag taacaatcag ggcccgggaa aaattgttcc ttccagtttg
     6961 cgctcctcgc aatctgggca ggcgaagatg gagggccctg cgaccacggt cttttcgggg
     7021 acgatcacgt tctgatacct tttttgttcc tcctgtaaga catctcctgt ctacccacag
     7081 aggggagaga atacgaagag gagataatag agcgacaccc cgagcatcac cacccccgcc
     7141 acgacatgga ttcctctgcg gtgccgggag aaccacgcga ggatctcgag gctccgggtt
     7201 ccagagacgg cagcgaatgc caggagcggg agggacatgc cgagtccgaa cgcgaagaac
     7261 gagcctaggt ttgcaagaaa gcccgtggca gtcgtcccga tcgcaaggag ggccaccacg
     7321 gccgcggcgt tgcatggaag gatgataacc ccgaagaaaa ggccaagcag gaaagcgtcg
     7381 aggaatggtc tcccgctccg gggctgtgtg atgctcccag tccaccggct gagatcgata
     7441 tcgagaatca ggacgattga aaaggcggcg aggaccagga atgcgagcgg ggagaggaac
     7501 gagaggaacc gggagaggga gacctgcacg accgcaacaa agatgatgcc gaacacgagc
     7561 atggagagaa tgactcctgc caccactatc atcccaaggg tgagcgtgga gcgttcgcgt
     7621 tcaccttttc ttcccgcgag gaacgagagg aaccctgggt agagtgggat gacgcaggga
     7681 gagccgagcg gggcgtaaag gccggcaagg aacgccatcc accaggccga gaggatctcc
     7741 accgctcagc acaccgccct gatcaacgat gagagctggg ctgaggtctt gatgccggta
     7801 ccaaggagcc gggcatcccc gccaggacag accagtatca caggggctga ggcgggtgtc
     7861 accaccaccg ggccgaaccg ttcggtgaga gagccggcca ttcctggcgg ggaaagggtg
     7921 taataaccca ggaaaccgtt cttttcgatg tggctcttca gaacagcctc ctcctccttt
     7981 ggatcgatgt caattccgat gacagcaaca tcttcgccaa gctccttctt gagggtcgag
     8041 atctccttct gttgcagggt gcagattggg caggagacag tgaaggtata caggattagg
     8101 ggtttcgcgg ataacgaggc cacggagaat gtctcccctg tctgcacgtc acggagttcg
     8161 atgttcctcc agttctcgcc ggtcatggtt cctccattga catgaccagt agttcccatg
     8221 caccctgcgg agaagattat gcagcacgtg gccagaacaa ggcctgccgg ggggagaacc
     8281 ggcgagaact tcagcacgtt cataccagta cagctcgaaa atcgggggtt aaataccttc
     8341 cttcttcggg ttatgcggcg gttcccaggc gcggatcccg ttctgcgtga tctcgaagtc
     8401 gaaccaggct ccaggaggga gggagcggtg ctttgcgagg gttgcccggc ggaggtggtc
     8461 cctcctctca aggcggatga tggtctttga gaggtgctcg agggcggtgc ccccaaggcc
     8521 gacgaagacg ttcctcgtgg tgtcggtata gacctggttc gagataagca ccgggatgcg
     8581 gtacctcctc gcatacccaa gcagcatcag cacctgcccc gtcaatttct gcatggcgtc
     8641 gcggccacgt tcgagctggc tccggtagag acctgttgca gagtcgacca ccacgaggcc
     8701 ggtcccccct ccgcggagca tggcatcgag gctctttacc atcacgccct gctgcgtgaa
     8761 atcctcgggt tcatagagat agagtcctgc agccagtttt tcggcgtctt ctcctgcgat
     8821 ctgccggaag cgctcggcag aaaagccctc gctgtccagg aagagcacct ggtctcctga
     8881 acggagcgcc gagaccgaag ccatcacgca gatggtgctc ttcccgctgg ccggttcacc
     8941 gaagacctgg gtgatcactc cgcgtgcaag gccgcccccg aggatctcgt caagatcgac
     9001 agagcccgtg gagaggggag ggggattcat tcactcatcc tgcccgccgc cttccaggcc
     9061 tctgcttctg ccagccgggc aacctcgcgg gcggggactg agcactccct cgccacttcg
     9121 aacacgcgat cggactcggc cttgaccgag aaggcctttc ccccaagggt cccgcatttg
     9181 accgggacgt cccatcgcct gccggacaga tcgattgtga cgattcgtgt ctccctctct
     9241 gccacgaacc tgtggatgct cgggatgcag cgcacgccaa gggtgccggt ctcttccgcg
     9301 agggccaccg agagccgggc tgagtccccg ggaaagcaga ctacccgcac gagatagccc
     9361 gggcgccctt tcttcatggt gcagggaacg gcacatacgt cccgtgctcc cacactcatc
     9421 agcctgtcca cgcatcctgc aaggatctcc ccggtcacgt cgtcgatgtt ggtctccagg
     9481 atgtctacct cgtctccgcc cgaaaaatgg gatgtctcga tgagggttgc acggagcacg
     9541 ttcgggatgg aaggcggatt cctttccccg gcaccatacc cggtggcgac caccctgccc
     9601 cggggaggaa cgccgggaaa cgcggtggca aactctgcaa ggagtgcagc gcccgtggga
     9661 gtgcaaagct ccccgtcttc gcctgttcca tactgcacag cgaggccggt tccctggagg
     9721 atggcgaggg tcgcaggagc cgggacaggg actgtcccgt gagcggtgcg gaccgtcccg
     9781 cccccgacag caatagtgcc gacggcaacc gcatccacgc cgagagagga gagtgcagtg
     9841 caggccccga cgacatcggc gattgcgtcg tccgccccca cctcgtggaa atggggacat
     9901 tcgccatgga tgaccgcctc ccctgcagcg atcctctcga acaccctgca ggccattcca
     9961 atcgcccccg cgggtgcatc tgcgccgttc acccgttcaa ggacctctgt caacgaccgg
    10021 agcaggaccg gtgcccgggt gtccaccttc acggcccgga tccctgcccg tgtcacctct
    10081 ttgatggagg gttcggcgac gaccgactcc atcgcccggg acacgaggtc cgcgcctgca
    10141 ccaaggtgca gcagcgcccc gcagatcatg tcaccggcgg ctccccggaa cggatcaaag
    10201 accaggactc gcatacgtac ttataaatcc cagcgtattg cacctttaaa ttaatgcctg
    10261 aactccatct gaaggttgac agcgcgtatc cgggcgatca gggcggcgga aaggcgaggc
    10321 tcgacccaga gacaatgctc tttttgaaga tctcccccgg cgacatcgtg gtggtggaag
    10381 ggaaacgccg gaccgtggcc aaggtgtggc ggtctctcgt ggaggactgg aaccagaaca
    10441 agatacggat cgacaacttc accaggatga acgccggggt cagcatcggc gatactgtca
    10501 ggatctcccg cgtacaggac gaaatcgagg caaaacgggt ggtgcttgcg ccccccgagg
    10561 acctcccgcg gaacgtcccc atcaacatca cccctcacat cgttaatgct ctcatcgact
    10621 tcccggtggt gaagaacgac cttgtgcccc tctcgagcgg cctgccgttc ctccagacgc
    10681 agttcgtgcc gttcaaggtg gtggagatcg agccagagca ggcggtgatt atcaccaaga
    10741 atacccacat cgagttctca gagaagccgg ctcccggagt tgagggggtc aagcgcatct
    10801 cttacgagga catcgggggg ctgaaagacg agctgcagcg cgtgcgggag atgatcgagc
    10861 tgcccatgcg gcaccccgag ctcttccaga aactcggaat cgagccgccg aagggagtcc
    10921 tcctcttcgg gccccccggc actggaaaga ccctgattgc aaaggcggtc gcaagcgaga
    10981 gcggggcgca cttcatctcg atcgcaggcc ccgaggttat ttccaagtac tatggcgaga
    11041 gcgagcagaa gctccgcgag atattcgacg aggcagagca gaacgccccg tcgatcatct
    11101 tcatcgacga acttgactcc atcaccccga ggagggagga ggtgaccggt gaggtggagc
    11161 ggcgtgtggt cgcccagctc cttacgatga tggacggcct cgaggaacgc ggccaggtgg
    11221 tggtgatagg ggccacaaac cgcgtggacg cgattgaccc cgccctgcgc aggcccggga
    11281 ggttcgaccg cgagatcgag atcggggtcc ccaccgagcc cgatcgcatc gagatcctga
    11341 agatccacac caggggaatg ccgctttcgg aggaggtgaa cctcgagcag ctggcccagc
    11401 agacccacgg gttcgtcggg gcggaccttg cagccctcgc gagggaggcg gggatccggg
    11461 cgctgcgccg gtacctcccc gagatcgacc tcgacgagga ggagatcccc gaagaggtgc
    11521 tggacacgat ggaggtcacc gccggggact tccgcgatgc gctgcgggac gtcagcccga
    11581 gcgccatgag ggaggtgatg ctcgaggtct cgcatgtgac gtggcaggac gtgggcggcc
    11641 tctcggaggc caagcaggag gtccgcgagg cagtcgaact ccctctcacc gacagggaac
    11701 gattcgagga actgggcatc cgcccgccaa gaggcgtgct cctttatggc cccccgggta
    11761 caggaaagac cctgattgca aaggcggttg cgaacgagag cggggcgaac ttcattgcaa
    11821 tccgcggccc ccagctcctc tccaagtggg tgggggagag cgagcgtgcg gtgcgggaga
    11881 tattcaagaa ggcccgccag gtggcgcccg ccatcatctt cttcgacgag ctcgatgccc
    11941 tggcccccac acggggcggc gaggtgggaa ctcacgtgat ggagagcgtg ctgaaccaga
    12001 tcctgacgga gatcgacggg ctcgaggaat tgaaagacgt cgtggtcctc ggtgccacca
    12061 accagccgat gcttgtcgac ccggcgcttc taagggcagg ccggtttgac cgcctggtct
    12121 acatcgggga gccgggaatg gatgaccggg cgcgtatcct ggccatccac ctccgcggaa
    12181 tgccggtcga gggatctgcc atggaggcac ttcttggctc tcttgaaggc tttgacgagg
    12241 ggtcaatcga tgagctgggc gaggcgctcg gccgggagaa gactgtctct cccggggagt
    12301 ttgccaggat cgcaaagacc ctcaaggtcc cggaaaagag ccccctctcg cggtcccagc
    12361 ggcgccgata cctgtccgaa ctgatgatct cccgcagtct caaggtgccg gacgaggcgc
    12421 tcgaggaact ggtgcgggat atcgcagccc gcacagaggg atacgtgggc tctgacctgg
    12481 aggcactctg ccgggaagcc ggtgtctttg cgatgaggga agggtcccgg caggttgcga
    12541 agcggcactt cgacgagagc cttgcgaagg ttcatcccac gatgaatccc cgcttaacag
    12601 agtattacga gcggatacgc cagtccttca agggcgggct cccgaaagag gtccagcccc
    12661 cggagtacca gtagcggctg gagagaaaag agggcgttta atatcgatac ctgacccatt
    12721 tttccctcag gtcttccatg cagaacctat cggccttcct cgacaaccat gccctcgacc
    12781 cctcggcgtg cgcaattgtg tggccggcga ccggcgagag gtgcacttac caggacctcc
    12841 tctcgcgggt gcagcgactc gcgtcggcgc ttagtgggag gggaatatcg aaaggcgacc
    12901 gggtctgcat ctatctcgac tcctgccccg agtaccttgt cagctacttt gccatctggc
    12961 ggacaggtgc cgtggcggtc ccggcaaata tcgtactgaa agagggtgaa ctgcgctatc
    13021 tcctccagga ttcaggcgcc cgcgggatca tctgcagcag taaggggtat cctgtggcca
    13081 ggaccgcggt atcctcctgc ccctctctga atctggtggt ctgcacgggg gatgcaccgg
    13141 aaggcacggt cccgtgggac ctggtaatgc aggaggagcc atcgacagga agtgcagccc
    13201 cctgcgggtt tgacgatccc tgccagcttc agtatacgtc tggcactacg gggaggccga
    13261 agggcgcgat cctcacccag gggaactgga tggctgcgct tgaggcagag agggaggtac
    13321 tcgggcttcg ccccggcgac aggtacctcg ggatctaccc catggctcac gtgggggtat
    13381 cgtggggaat ctcggtcctg cgggcaaagg gaacgtacgt gatcatggag cggtttgaag
    13441 aagaggagta cctgcggctc gcaagagagc accgcatcac tgtcctctcc gcgatgcccc
    13501 ccgttatcca cgggctctgc gatgccccga aggaggcaga ggaggcgctt gggaccgtgc
    13561 gggtgatcat cagcggggga ggtcagctcc tgcccgccgt ctgggaggca tttgacagga
    13621 gataccggat tcccatcgcc aactcgtacg ggctctccga gacgattgtc gtcgggaccg
    13681 ggactgcgac tgtccccggg ttgccggagc tgacccgcga ttaccggagc gtggggattc
    13741 ccatcgggta cagcgaggtc cgtattgttg acagctcgga tccatcagtg gtgctcggac
    13801 cgggcgagac aggcgaaatc gccctccgtg ggccatcggt ggcaatcgga tactggaata
    13861 tgccgagaga gaccgccgag acgttccttc cagggggctg gttcctcacc ggggacatgg
    13921 gacaccttga cgagggaggg gtcctctcca tcacggacag gaagaaggac atgatcgtca
    13981 tgtcgggatg gaaggtgtac ccgaccgagg tggagaacat cctcctccag cacccggata
    14041 tcgcggatgc ggcggttttt ggcacacctg acgagaggaa aggcgagatc cctgtcgccg
    14101 cggtggttgc gagggaaggg tgccggctgg acggggagga tgtgtcggcg ttctgccgga
    14161 gccgcatggc gggatacaag gtgccgagga gagtcatctt tgtcgaatca ctcccccggg
    14221 tccatggatg gaaactcctc cgccgcacgt tacgggaaga gttctcaaat acgtcctgat
    14281 tttgtgcaag gctataagtg aaaggacaga aatagtacgt tttggagata tcccctttgg
    14341 ccaaggacaa caggcgatcc actggcattt ctggcctgga tctcgccatg gagggaggct
    14401 tccccctcgg tacatcggta gtagtccagg gttcgccgct gaccgggata gatcatatgg
    14461 cccgccagtt ctggggaagt gacagcagtt cggggagtta cctgatgatc gacggggagg
    14521 tcgaggaagg catggtcccg gctgcaggat tgactattga ccagctggca gaacagtgca
    14581 ggggcgacaa gatcgtcgtg gactcgctct cgagcatcat catcaccagc ggaatcgata
    14641 ccgccatctc ttttctcggg agggccaaga aggatgtggc agcacggaac gccaacatca
    14701 tgctcctcct ctatcccgat ctccaccctt cccacgagga gatacgcatc atgcgtatgg
    14761 ccgacgtggt gatcgagtta aaagaggtca tcttcatgaa cgagatcgag cgccagctcg
    14821 cggtgcacaa gatccggagg ggccaggtgc caaagcgcct gatcccattc aacatcactg
    14881 ataaagggat agagctctcg accacatcga gggttgtatg aacatcgctt cagcgatgtt
    14941 cattgggtgc ttttttggag ttgcacccct ccaataacca agaggatatc gcttcagcga
    15001 tgttcattgg gtgctttttt ggagttgcac ccctccaata acnnnnnnnn nnnnnnnnnn
    15061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15121 nnnnnnnnnn nnnnnnnnnn nncaagagga tatcgcttca gcgatgttca tccaagggtc
    15181 cccctcacct cccgcactgc ggcgaccatg tgggagagct tggcgaacgc gacatccttt
    15241 ggaagcatcc gcagtccgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
    15361 ccatgtggga gagcttggcg aacgcgacat cctttggaag catccgcagt ccgcagtcgg
    15421 ggtcaatgag gagaaattcg gggccgagca ggtccactgc gtgctcgatt ctttcccgga
    15481 tgacactgac aggctctacg gctgtgtcgc ttgagtctac gcagccgacc ccgagccgtt
    15541 tgccgttgag ggcgttcctg tcaaagagtt cgaggttttc gggacttttt gcacactcga
    15601 tatcgatcat ggcaatggga aacccaagga gccgatcgac gatccccgag aggtctccac
    15661 agacgtgaag ggagaccggc acctggagtg ttgaggtcac gcgtgagagc gcttcttctc
    15721 ccgcatacag gtctgcggct cccgtcgaga ggatcggctc gtccacctgg accatgcagg
    15781 ccccggcccg ctcaagggca agggcctcgg ggacaagggc ggccgcaagg tcaagtgcca
    15841 ggtcctgtct gtccctatag acaggggtgg cgatatgcag gccgcaagag agcgtggtcg
    15901 gacccgtgag gatacccttc accatcggat ggcaggaaag tgcgtaacgg gtgtcattga
    15961 cggtgatagg ccccgatgcc gggagcacct tgttcaccac gtcctgcccg cggataccgg
    16021 ggaggcggga agaaaaggcc tggatcatgt ccccacggac ctgaccgtcg gagatgatgt
    16081 cgatccctgc ccggacctgt gcgtccaccg ccaccttcac cgcgtgcctg aacgggtcga
    16141 ggagcccccc cagccccgag ccccgctcca cgggaaagct tcccacgacc gtcgtgggga
    16201 ggatcgggag gcgggcgaac gggtccatgg aagatcaggg tacaaggcgg tgccggtcgc
    16261 gggggaagag gactgcttcc cggatgttct ggagcccgag catggtcatg accagccgct
    16321 cggccccgag gccccagccc gagtgagggg gcatcccgta ccggaacggg gtgaggtaga
    16381 atgcgaaact gtcggggttc agtcccttcg actggatctg gcggacgagg agatcgtgct
    16441 ggtgcacgcg ctccgaaccg gacgagagct ccatccgcgg gtgcataagg tcaaatgccc
    16501 tgcacaccga tgggtcgttt tcgcacggca tcgcatagta gggcttgatc gcagtcggcc
    16561 agtcgacgat gaaatagtga cagcccatct cctcccctat ggccttctcc gccgcggtac
    16621 cgatgtcgtc cccgtaggtg atcggctcgt cggtcttctt ctgggcgatc gtgatcgcct
    16681 cttcgtaggg gaggcggggg aaggccgagg acgggacctc gaaatcgggg aggtccagca
    16741 gttcaagaaa cgggcggcat tcagagtcga cccgccggta tgccgcgatg acgatctctt
    16801 cgaggagcgc catcacctgg tggtggtcgg cgtacgagac ctcgacgtcg atcgaggtcg
    16861 cctcgtttaa gtgtttcgtg gtgttatgct cctcagcccg gaagattggt ccgatctcga
    16921 agaccttctc aaatccagcc cccatcatca tctgcttgta aagctggggg ctctggttca
    16981 ggaacgcctc cttctcgaag tacgcgatgg ggaagagctc ggtccccccc tcggttgccg
    17041 cggccaccac cttgggcgta gtgatctgga tgaacccgga agagaagagg tgttcgtata
    17101 tcgcccgcat gcaggcgttc ctgacctgga agaccgcagc aaccctggga cgcctcgcat
    17161 caaggaagcg cgaatcgagc ctggtatcca gttctgcggg taccttctcc acgacatcaa
    17221 gggggagcgg gctctctgcg aggctcacaa tctcgaaggt ctcggggacc agctccctgc
    17281 cgcccggtgc tttgtcgatg gccttcacgg tccctgccac cctcaccacg gactcgcggg
    17341 agaccagacg gactgcggag agcacggcct ccgggacttt cttcttgggg acggtcacct
    17401 ggatgatccc tgtgcgatcc ctgacgagca ggaaggacag tcctccaagg tcccgcaact
    17461 cgtgcacgaa acccacgatc tctgcccttt ccacgccggg agtcacctcc tgtatcggta
    17521 tccgcatatg aaatccaata cctatggtcc gtgattgata atcttgtttt cctgcgctgg
    17581 ctggaagaca accacgggac cgggggggga aacccaggca tcctgggtgg tcacctcact
    17641 ctgtcattcg gtcccgaata tctcctgcct gatggcctcc tccatgccgg cctgcgcggc
    17701 aaccgcggct ttcaggtgcc cggggctctc ccagacatac cagtcctgca tccccatcat
    17761 cacccaccag cgggagagca tctcccgtga aagcgcgaag agctcctgga aatatcccgc
    17821 gtccaggtcg cgttccatgt ccatggtgac ggtcagcttg aggatgttcg agagtgcatc
    17881 gcccaccatc ttggtatggt accatgggga acggctccag cggtggctca tcatctcctt
    17941 gacgtccagg ttcctgaacc gctggagctc ggggatgttg atgaccgggt tcagcttata
    18001 ttcggagagc acgtaggcga gcagcatgtc ccttgcataa tacatcccgt tatgaaggac
    18061 aaaaaagcgc tggggcagaa attccttgac cggcaccagg tcgtgcatgt cacgcatgct
    18121 ctcttttaag aggaagtaga cctgtgcgtt ccggatgacg gggtatctcc tggcaatcac
    18181 gatgtttgcg aggagggtga gcggaaggtc cgagaatgcc tcgtgaaaga gcatgtagag
    18241 gaggaagacg agctgcttct tcctccagac cgcaaggttc cgggtacctt cgtccttgag
    18301 gatgtcgaga tagacctgcc tgcaggcggg gagcgcacag tcctttgaga aggagatgac
    18361 ccggtattca ttgtgttggg cggcacaccc ctttgcgagc agctccaggc agtggcggca
    18421 gatggtataa tccttgagca tcccgagctg ggagcttgag aatgcgatca cgtgcttccc
    18481 ggtcccgaac gtgaaatcgt cgataaggac accctggggt ggaacgtcgg tccgcctgac
    18541 gagctggatc tcggtcccaa gggacgtttc tgcgaactca aggagttcgc gcaggacagg
    18601 ggagaggttg taggactggg tcatccgtca cttccagcga gaaggcatgc cggaccgggt
    18661 cgcatctcaa ttagatgtaa cttcgtcacc gatgatacat aaacgatacc aaacagaagg
    18721 atccagccga aagaaagggg gtctcacctt gccaggcggg cgtggagttt cctgattgca
    18781 ggctcaactg tggggagata gacattcagc acgttccata cgaggcccca gtcaggggag
    18841 aaataggggt ggatcagccg ggtgcggagg ccctcgagtt cattccagga aaccccgcgc
    18901 gagtcctgcc tgcacgcgcc cgataccctc gccgccgcct ccccgatgat ctccatgctc
    18961 cgtgtgaccg cacgcctgag ggtctcgtct ttctggaagt ccttggccga gacacccttt
    19021 gtccggtggg agagataggc gatctcgtca aggatctggc cgagatacac cttgtcgcgg
    19081 gagagcgcgg caaattcggc cccaagttcc ggacggaggt acgaggcaag gacgcgggac
    19141 atgacaaggt ccaccctcct cactaaaagc tcgtccaggt acagtgagag cccgatgtag
    19201 tttgcatagg tgtccatccc cgactcgaac tcgacctcga tgtccacgtc gctttccggc
    19261 cgttcatcac cgcgggcgac agaaccgaag agccctatct tctttacccc gtaaagcctg
    19321 cgcatcactg cgaacttttc attcaggacg aggataacct cttccctggt tgacggtctc
    19381 tcctgtggcg gcattgtgca cccaatccct ccttctcctc attcctatgc atgccatggc
    19441 cttatgggta tcgtcaatgc atgcggggtg ccacgggaca gggcacgaac cggcccccga
    19501 accttcttta tcctgtcatg ccccctcttg atcatgcaca tcccagagat cctggcactc
    19561 gtcatgatca cggtactgct gccagctgcg ttctcagggt gcctcatgca gccgggaacg
    19621 gaattgggag aagttcccac ccctcccccc gcggggctcc cctctcccca cggcacggcc
    19681 gtgggagtgg aggtggaggt ggctgcagga ccggcgaaca tgaccctgac tttcctgccg
    19741 ggaggcgagt tttgggcggg agaccggata ctccttgcag ggaccacgaa cctctcgcca
    19801 ggaaatcacc tcctggtcga ggttatcccg gtctcgtttg gacccacgcg gaagggtgag
    19861 caggtcgcag tatcagggag ttccggtgtc gtggaagtcg tggcagggaa gaatgggctg
    19921 ccgaatgcgt ggtcctttac aatcgatacc cggggatggg agccggacga gtacctggtg
    19981 agggtgcagg ggatcgaggt cccggcatcc cttctcacct cccggtttac gcttcgtccc
    20041 tgatattccc ctggttttcc ctcaccagga gccaggcttc ttcccttctc cctgcttctc
    20101 cggatcctgg tccggttgcc gggggacagg tccggtaccg agcgatccct ctccctgctt
    20161 cttccttatc ttctcaagcg cctcgaaccc ctctctccgg accgatggca cctggtcgga
    20221 caggagcctg gtgagcccgt cgactgcggc tgcgtcgcct atctccccga gaaggagcgc
    20281 ggtcctcctg cggacctctg agttctggtc tgagaggagc tcgagaagcg ggcgggtggc
    20341 cggcccccct atgagccaga gcgcttccat ggtgcagttc cgcaccacca cgtcaccctc
    20401 ccggaacgct gccacgaggg gggtgattgc gggatacccg agcgcggcaa gcgccctgac
    20461 cgcctcctgg cggactttcc tctccttatc gccgaggacc cgaatcagcc cgggtatggc
    20521 ccgcgggtcc ccgatcctcc cgagcactcc cgctgcgccc tgcctgacca gtgagtcccc
    20581 gtggctgagt gccgcgatga gcggttccac cgcgagggtg ccgatcgagg cgagcgcaag
    20641 ccccgcacgc tgacggatct cgggctgcgg gtgggcgaga gccgccacaa gggggtcgat
    20701 gctccttcct cccagggaac agagcgcctc ggccgcggag atgcggacat agccggactc
    20761 gtcgccgaga aggcttatga gcggggcgat cgcctcctcg ctcccaagca gtttcagggc
    20821 cgccgccgcc tcccgccgca cgccagggtc gctgtccttc agtgtctcga tcagcggtcc
    20881 ggttgcggcc tggacctgca tctcccccag tatctcggcg gcaccgcggc gtatcgcggg
    20941 gttctcacgg gagagggcac gtatcagcga gtccacggca ggctcccctg cacatgcaca
    21001 tgcacgcatg gcctcctggc ggaccgactc gtcttcgctt cccagcatct cgatgagagg
    21061 gggcacggca gggaggccga tcctggcaag ggtctcccct gcacccctcc ggacaatcca
    21121 gtgctcgtct ttcagggcat tgacaagcgg gtcgatgaag gcgtccccga gttcacctgc
    21181 cgcccggatc gcgttccagc gggcctccac gtccccgtag gcgagtgccc tcctcacgct
    21241 ctccacgtcc tccctctcca cctcgtccat ctggtgcgcg ctattcccgc caagcgctcc
    21301 cttgagtgat cgaaagatgc tcatccgggt cctcttcctc acacctgtgt ctgccgcgca
    21361 ggtaaaaaac cctttttgtt gccgggggaa gtaaaaccgg cctttcacgt cccccgtaca
    21421 agggagagaa tccctgcggc aagtgactcg tcgaccccct cgcggttcat cagcgtgtcc
    21481 atccgggtcg cgcctggaac gcccaccgtg tcccgcgtgt cctgtacgaa gaagtccagg
    21541 aacctggcat agagatccct cgttgcggca gaatcggggg caaaccccct tgcacgcatc
    21601 aatgcggcgg caggcccgct taccggcgca tccccgatga aggggctcac tgcgaccacc
    21661 ggcacgcttt ccagggcttc aacgactccc ccgcattcaa ggataggcag gatgctggtg
    21721 attgggttgc tgggcccgat gatcaccgcc tccgcggaat tgatcgcgga gaggacctcg
    21781 ggggtggccg cgggaggcgt ctcccatctc ctctttacct cgtcgacagg gagcgttccc
    21841 ctgtgcctga cccagtactc ctggaagtga atttcgcgcc cacccgtcat gatgatggtc
    21901 tcgacctggg tattcgtcat gggcaggacc tgtgccctga ccttgaggga cgcgcacagg
    21961 tgcgcggtcg ctctggtgag gttcatcccc ctcctcagga gttctccccg caggatatgg
    22021 atggcccggt cgcggtcgcc tatggcgacg tattcaccgc tgtccaggcg cgtcagttca
    22081 tcgtgggtgg tgaacgtgtc cccctcctgc ccccaccacg acgtggtgtt gagcagtccc
    22141 gagaagaggt agatcagggt atcgatgtcg ggcgacagga agttcccgct catccacagg
    22201 tcctcggcgg tgttgacaac gacggagatc tcgtggtcgg gaacatgccg cctcatgccc
    22261 cggaccagct tgggggtccc ggttcccccg gaaaggaagg tgatcatact tctatactat
    22321 ttataggagg gtgagaagag tgttgtttcg gattgggcat gaagatcata aaggaccctg
    22381 tgcatggcta cgtggaagtt ccgtcgtcca tggtctccct ccttgactcg gctcccatgc
    22441 agaggctccg gtacgtgcgg cagctcgggt tctcctacct cgtataccct ggcgcccacc
    22501 ataccaggtt cgagcactcc ctcgggacga tgcaccttgc aggggtgatg gcccgacagc
    22561 tcgaccttga cgcggatgag cgccttcttg tggccgcggc ggggctcctt cacgacatag
    22621 ggcatgggcc cttctctcac gcaatcgagc cgctctcgga ggagttcgca gcgaagagcc
    22681 accaggacgt cgaaggactc ctggagcagc cggagaccgg ggggatcctg gaacggatgg
    22741 gtgtcgatcc tgacggggtg acctccatga tcaaggggga gcatcccctc tcgggtatca
    22801 tccacggcga gcttgacgtg gaccggatgg attacctcct gcgggacgcg cactataccg
    22861 gggccccgta cggcacggtg gatgcccacc ggctgatccg gagcaccacc cttgcagatg
    22921 gcagggtggt cctggaggag gccgggatca atgcggccga gtcgctcctt attgcccgca
    22981 ccctgatgcg ccccgcggtc tacttccacc atgtgagccg gatcgcggag atgatgttcc
    23041 agggggcggt gatggcgcac gtcgagagta atgccaaaag taaccttctc tcgctccttt
    23101 acgatgacga tgctgcctgc atgcaggctc tcctcacctc cccctcgccg gtcgcgcggg
    23161 atctctcccg ccgaatctac cagcgcaggc tctacaagcg ggcgatctac gtgaaccgca
    23221 accaggtgaa cgcggcgaga gttgcccagt gctccccgta tccgaagaga gcggcgctgg
    23281 ctgcaaagat cgcggccgtt gccgggctct ccccccacga ggtgctggtg gacatcccgc
    23341 cgttccctgg cgacatgtcg cttcacgtcc aggtcaggaa ccgtcacagg gctgtcggca
    23401 tatcggaact ctccccgctc cttcacaccc tgaacgagac ccgcaggggc cagtggcggc
    23461 tcggggtcta taccctcccc gagcacaggg acgaggtcgc gggtatcgca acagacgtgc
    23521 tccacgtgag gcccgagacc atgcaggaga ggctctttgg gtgagcctct ctgaaatgga
    23581 tgcgagagag cgaaaaagaa tctttaagaa cgcctgccgc caagagagag cgagggggtc
    23641 ttcattgatc gaatactgga tcgtggtcac caccggcgtg gccgctgtgg caatcgtcct
    23701 ctgcggcgca tatctgtaca atgagataaa atcgcgggag ttcgtgcaga acagggtgaa
    23761 gctgaaggta tacgtggcac tcctgcaggc ggtgaccgag atcaacgtcg ccggaaacga
    23821 tgcatacgcg ctcgatcggg caaaacggaa ccttgcatcc acgctgaacc agatcaacct
    23881 gatcgccggg ccgcaggtgc tcctccacgt caacgagctg cttgacttcc tgaacgagac
    23941 gcgggaaggc gattacgatg tcctcaagga gaagaacatc ctgaactcca tcgtgaaggc
    24001 ggcgaggagg gaacttgacc ctgcgagcgc acgggcgctt gaagagtccc agttccggtt
    24061 ccgtttctac aacccgcccc ggccctgaga gtcccgtcct atttcttttt tcatcccggc
    24121 ggtgcgttcc caggcaggtt tcgggggctt gtagatttat cagagtgcag ctcacacctt
    24181 atcgcatggg tgggaggcaa gactgcaatg cgtgaattca tcgagcagat gcgggaacgg
    24241 tccctggtcg tcgatgtgga tgagcggccc cggggagagt tcgacgcacc aaaaatggcc
    24301 agcaccaccg attcgctcct cttcttccat gatctgcccg gcggccgggc gatcatgaat
    24361 gtcacggcca caaggaaggc cctctccctc gcgcttggcc ttggagagag agagatcggg
    24421 atgaaactct cgaaggcgtc ctacgacggc agcgtggtcc gcgaagggtc gctttcgatg
    24481 cgtccggcgg acctctcccg catcccggca atgaagttct tcccaaaaga tgcggggaag
    24541 tacctcactg ccgggatcgt cttctcgggc tacgggggag tggagaacgc ctccatccac
    24601 cgcatgcttg tccttgacca ggaacgggtg gcggcaaggc tcgtcgaggg aaggcacacc
    24661 tacacgctcc accgggaggc actggcccgc ggggaggagc tccctgtggc cgttgccatc
    24721 ggtgtccacc cggcagtgac cttcgcgagc tgctcaaggg tcccgaaggg aaaggagctc
    24781 ccctttgccg cagagctgct gggcggcgag gtacgggtgc accagtgcag caacggggtg
    24841 ctcgtccccg aagcagagat cgtgctcgaa gggtatatcg ggagcgatac ggcacgcgag
    24901 ggaccgtttg tcgacatcac gggaacgtac gacagtgtcc gcgtccagcc ggtcatccgg
    24961 ttcaccggga tgcataccaa gaatgactgt atctaccaca gcatcctccc cgggggcaac
    25021 gagcaccgca tcctgatggg ggtcccctac gagcccatca tattcagggc ggtctcggcc
    25081 gtcaccgagg caaggaacgt ggtgctgacc accggcgggt gcgggtactt ccacgcggtg
    25141 gtccagatca aaaagagcac ccagggggac gccaagaatg ccatcatggc cgcgttcgcc
    25201 gcccacacct ccctgaagca tgtggtcgtg gtcgacgagg acatcgatcc ttccgacccc
    25261 gcggaagtcg agtttgccct tgccacaagg gtcaggggtg accgggacat cttggtggtc
    25321 accggcgcaa ggggctcgtc ccttgacccc tgcagggtgg gggacgggac caacgtgaag
    25381 gtgggagtgg acgcgacgat ggtcctcggc caggaggccg agttcctgcg tgtcggctgg
    25441 caggaagggc aatgatcctc tcggctgaag aggaggccat cctcgccggg gaagagggcg
    25501 agacgagggc caggatgctc gagatcctct tggcgctggg gaaagtctac ggcgcggagc
    25561 ggctgatccc catccggagc gcccaggtga gcggggcctc ctacaagacg ataggccgct
    25621 ttggcctcga ctggctgcag cagctcgaca caaaggtctc tgtcccgacg gtcctcaacc
    25681 cggtgggcat ggatcgctca cggtggcggg agatggggat caccccggaa tttgcccggg
    25741 tgcaggagca ggtgctggat gcctacaggc gccttggggt gaccatggag tgcacctgca
    25801 cgccgtatta cctgtacatg acgcggtttg gcgatcacct cgcctggtct gagtcttcgg
    25861 ccgtcgcgta tgcaaactcg gtgatcggtg cgaggacgaa ccgcgaaggg gggccgagcg
    25921 cactcgctgc ggcgatcatc ggcaagacgc ccgagtacgg gctccacctt gtcggggcaa
    25981 ggaagcccca ggtggtcatc cgcgtggagg agggtgaccg cctcgttgac gcggcacact
    26041 tcggggcact cggatacctt gcaggcaggc tcgtggggag tcgtatcccc ctgctggagg
    26101 gaatccggcc gaaaaaggac ctcctgaagg cgctcggcgc cgccatggca gcctcgggcg
    26161 cagtcgccct cttccacgtg aaagggatca cccccgaagc ccgggtcttc tcctatccca
    26221 cagaagggct ggaggtcatc gagatcgggt atggggagat atccgcgctc atgaaggaga
    26281 tccccgtcga tgcagtcgcc cttggatgcc cccactgctc gccggaagag ctctccagga
    26341 tcgcaggact cctggccggc aaaaaggttg ggatccctgt ctttgtcttt gcggcaaggg
    26401 atgtcgcagt gcaccaccct gccgaggtcg cggcgatcga ggagagcggg gcgcacgtct
    26461 ttgcagacac ctgcatggtt gtctctcccg cgatggagcg gttcggcgca atcatggtga
    26521 actcggggaa ggcgtttgcc tacatcccga acatgtgcgg tgcggcggta aggctcggga
    26581 gcaccgagga gtgcatcgaa gtggcgacgc ggccagctgc aggggcctcc tgaacacggc
    26641 gcattcgcgc cggggaccgg gcacatctct ttgttcccgg tggatttatc aacgatttta
    26701 tgcaaatacc caactgaatg gcattctcgg acctgcacct gaacacgatg gaccgttaca
    26761 aaccggccat cctcctcctg ctggtcgcgg tctccgtcat cctcgaggcg gtcgtgcacc
    26821 atgcgatggg gatagaggtg gtctactccc acttcttcta catccctgtc gtcctcgggg
    26881 cgatctggta cgggaagaag ggtgtcctgg tcgcggtttt cctcgcggcg gtctacgccg
    26941 gcgattacta tcttatcaac ggagtcgtgc ataccgacgc ggtaatgaga gccctgatgt
    27001 tcgtagtggt tgcgttcgtc atcgggatgg tgatggacta tatccacagg gagcaggagc
    27061 aggtaatgac ccagatggcc aacgccgccc tgggaggggg cacttccggg ggattccggg
    27121 gaaacctcgg ggaactgaag gcaaagctcc ggtcttcggt caacgtgaag cggctcaaag
    27181 aggaggggga catcgggggg ctcatcggtg ccctcggcca tcgggatgcc gcgatccagt
    27241 atgaagcggc cgaggccctg ggtgtgctga aggagccgtc cgcggtcact cccctgatga
    27301 cagccctgac cggcgaccgc tactcagggg tgcggtggaa agcggccgaa gcccttgcac
    27361 ggatcggcgg acctgccgtg gagcctctcg ttggcgcact cgggcactcc gacgaggacg
    27421 tgcggtggaa ggcagcgatt gcccttggcg agattggcga cgcccgtgca atccccccgc
    27481 tcgtcgcgct cctcgaagac caggaccggt ttgtccagag cagggcggcg tactcgctgg
    27541 ggcagttcgg caacgccgcg ctctctcccc tgctgctcgt gctggagacc gggagcccgg
    27601 ccgccaggag gggagccgca attgcgcttg gaaaggtcgg agaccccgcg gtaatggaac
    27661 cgctcctctc cgcgctctcg gatcctgacg aaggagtgcg ctcggcggtg atcgagaccc
    27721 tcgcaggcct cggggagggt gtgcactcac gcgtcctcgc tcatatccgc ggactctctc
    27781 caggagagag agaccgggtc ttccagtctc tccgggaggc gaccaacccg aggttcatcg
    27841 agggattttt accggtcctc tccatggcag atgaagacgc gaaagcggtt atcctctccc
    27901 tggtccgcca gggaggaagt ggggaacagg gagggaaaga cccggccccc ggtggataaa
    27961 cccctcctgc ttcccgctga ttcccgggtc ccggtcaaat atatgtgatg cgagtcccat
    28021 ctcttcctgt gtttggaata taccagatcc ctttccagac agacgcaccc ggaatcacag
    28081 tctctgtgga gagcgggttg gccacttcca cctaccggag gacctctcca gaagggaccc
    28141 tcgcaaagcg gcttgggtat cgggaaggga gggtgatcat caaccctgtc gagccgatca
    28201 accttcccac ggaggtgacg gggatgctcg agttccactt catgcctgtc actatgaggc
    28261 cgaactcacg gatcacgatc tgcctgaagt tcccggtgga gatcggggtg ttcgtggggg
    28321 cggatgacaa ttacacgctc ctcgacgtct tctctctggc caggcccaag tactcgctct
    28381 acggcacgcc tgaaaaggga gtcattacaa ggcacgtgga gtgcgaagtg tacgaagatg
    28441 ccccccccac gaaccccctc gaggaggggg tactctttct cacgctcagg aactcgggca
    28501 gggactggat cgaggtcacc cgcgccgttt tcgagagctc ggcgctccgg cttttctacg
    28561 ataagaccgt ggcgatggcc ggggagatgg aggtcttctc gaggatggtg gccgaaacaa
    28621 aggtcatcga ccgccccccc gaggagggaa tgaagcaggc cctcagggta tacccgacaa
    28681 aaaagagcct gatccccgag aggccgccgt tcatgatgga gtacggggta ggtgactgac
    28741 aatggcgttc gagtccctca ggatggaact gttcgggaac gtgaccctgc ttgacctgat
    28801 cgtctttgca atcgctgtct tcgttgccct ggtcatcgca aagatcgtgg ccatctacct
    28861 gaagaagtcc ttaagcgacc ggatcccgcc tgcagagatg gacttcctga tcaaggtcat
    28921 ccaggcggca atcgtcatca tcggggtata cctggcgctc ccgagtttca acatcaacct
    28981 cgcggacatg ctggtggtgg gcggaacgat ggggcttgtc atcgcgtttg cgagccagag
    29041 gattgtctcg aacttcggat ccggtctctt cctcatcgcc gaacgcccca tgaagcccgg
    29101 ggacaacgtc aacatcggca cggtgagcgg cactgtcgag gagatacggg tcctctccac
    29161 catcatcaag acgcacgagg gggtgtacgt cagggtcccg aacgagaaga tcttcacctc
    29221 cgagatcacc aatttcgtcg caaacgttgc gcgcaggttc gagtacacca tcgggatcag
    29281 ctaccagtcc gatgccgaac acgcaatccg gattgtcaaa acgctcctcg aagatcaccc
    29341 gttcgtgctc aggcacccct caccgagcgt attcgtcgac accctcgcgg attcgagcgt
    29401 gaacctccgg gtgttcatct gggcaccttc cagggtgtgg tggtcagtga ggaccgagat
    29461 gctctgggcg atcaagaagg cgctcgagaa cgagggaatc gagatcccgt tcccgcagca
    29521 ggtggtcacg ttcgcaccgg ggacggggct ttccctcaat cccgggtgcg gcggggaagg
    29581 agagggccat gggtgacccg gtgcttgagg gactcactga taccctcgta aacggcacga
    29641 tggcggaacg ccgcgaggca gttcggcggc ttgccggcag gggtgagggc gccatccagc
    29701 ccctgctcgc actcctccgc cagcccgtcg gaaacgatgc acgctggtac gccgccgttg
    29761 cgctcgtgaa gatcgggccc gctgccatcg ggcctctcat cgaagcggtc gcaggcaacg
    29821 atgaccagga cttccggcga tatgtcgctg ctgcgcttgg ccacctcggc gagccggcag
    29881 tggcaccgct gatcgaggcc atgcgggatg ccgatccgaa gatgcgggga ttcctggcgc
    29941 aggcgctctg caggatcggc ggtccggcga tccccgcagt ccggtcgatg ctcgggtccg
    30001 acgaccctgt ggtgagggag tgtgcgacac tcaccctctg gcagatgggc gaggagggga
    30061 tcgcgtctct cctctcctcg gtggacgaag agtctctgca atagcggtcc cccgcgaatc
    30121 cgggctgcgt cagatggcat ctgcaacgtg aaatgcgggc gtctcctgca ctatatccca
    30181 actgataagt ccctgccgcg gcaagagaga tggtgatgat gcagtgctct cccgggatgg
    30241 agcggtacct cgcggggctc caggaggggc tggaggcggc catggctgtc gctgagaaag
    30301 cgagggcggc agggatcgac ccccgcacca ccgtcgagat ccccgtggca agcgaccttg
    30361 cggacagggt cgaggcgctg ctcgggataa agggcctggc aaagaggatc cgggagatgg
    30421 agggggagat gtcccgcgag gaggtggccc tccacatcgg cgaggacttc gtgcagcgca
    30481 ggttcgggga ggagaccgac gaggagatcc tcgaccatgc catccggacc gcgatggcgg
    30541 tcctgacgga aggcgtggtc gcggccccga cagaggggat cgcaaaggtg ggcatcggca
    30601 gaaacgacga cggcagccgg tacctgaaga tctattatgc agggccgatc aggagtgccg
    30661 gggggaccgc ccaggcactc tctgtgctcg tgggcgactt tgtccgtcgg gcgctcggga
    30721 tgaaccgcta cgtcccgagg caggaggagg tggagcgtta catcgaggag atacgacagt
    30781 acaactccat catgaacctg cagtatctcc cttcggagcg cgagatccgg ctgatcgtcc
    30841 agaactgccc ggtctgcatc gacggggagg gaacggagca ggaggaggtc agcggctacc
    30901 ggaacctcga acgggttgag acgaacaccg tgcggggcgg catggcgctc gtcctcgcgg
    30961 aaggcctcgc cctcaaggcc ccgaaggtcc agaagaatgt gagggcgcta tcactggacg
    31021 ggtgggactg gctggacgag ctgataggcg gcgctgcaaa aagtggcgat ggtgacgacg
    31081 cggccggcat caagccaaag gacaagtacc tcagggatct catcggcggc cggcccgtat
    31141 tctcctaccc gatgaggaaa ggagggttcc ggctccgcta cggccggtcc cggaacaccg
    31201 gctttgccgc ggcgggactg aacccggcga cgatgcatat ccttggggac ttcctggccg
    31261 tgggcaccca gatgaagatc gagcggcccg ggaaggctgc cgggatcgtc cccgtggaca
    31321 gcatccaggg ccctacggtc aggctcaaat cgggagacgt gctcagggtc gacgatgcgg
    31381 tgcaggcaag ggagtgtgcc cccgaggtgg attatatcct cgatatcggg gagatcctga
    31441 tcggctacgg ggagttcctg gagaacaacc atccccttgt cccccccgcc tactgccacg
    31501 agtggtggtg cctggagggg ggggccggcc ggcccgggag cgaggaggag gccatcgcac
    31561 tctgcttcga gggggccttc ctccaccccg acttcacctg gctctgggac gacctctcct
    31621 gcactgacat cgcggaactg gcggactttg tctcgcaaac aggggagttg aggcacggca
    31681 tcctggtcct gcccccggac gagcggttca aagggatgct cgaggagatc ctgatccccc
    31741 accgcgtgag ggaggggctc gtctgcatca gcgacacgta tgtcttcctc gcatgccttg
    31801 gcctcgacct gaagctgaag aagcggcccg cgtggcaaaa cgcaccgatg gacgaccccc
    31861 tctctctcgt cagccacctc tcaggctttg ggatgcggtc caggggcggc acccgcatcg
    31921 gggggcgcat gggccggcct ggcaaatcaa ggccccggaa gatgaacccc cctccccact
    31981 cgctcttccc cctgggcgag gcggggggca gccgccgatc gttccaggag gcctcgaacc
    32041 atgccccgca gccgaacatg gacggagggg tgatcaccat agagaccggg aggaggagat
    32101 gcccctcctg cggcgccgag acgttccgga accggtgcga gtgcggggcg cacaccgaac
    32161 cggtctacag ctgcccccgc tgcaagcggg agcaggcggg ccccgggtgc ccctcctgcc
    32221 acacggcgct tgcctgctcc caggtcgtaa ccctcaacgt gaagcaggaa tacgcggcgg
    32281 cgatggagcg cctcggcctc cgggagagca gcatcgcgct cgtgaagggc gtcaaggggc
    32341 tcatctccag ggagcggacg gtcgagccga tggagaaggg gatcctccgg gctgcacgcg
    32401 acctctttgt cttcaaggac ggcacgatcc gcttcgacat gatcgacctg cccctcaccc
    32461 acatccgtcc cggcgaggtt ggagtcctgc ctgaacggct ccgggagctc gggtacgcgg
    32521 aggacatagc cggggagccg cttctctctc cctcgcaggt ggtggagctg aaagcccagg
    32581 acatcctggt ctcagactcg tgcgcggagt acatggtgaa ggttgcccag ttcctggacg
    32641 agctcctgga gaagtgttac ggcctgcccc ccttctaccg ggtgcagacg agggacgacc
    32701 tgctcggcca cctggtgata ggccttgcac cccataccag cgcgggagtg ctggcgagga
    32761 tcgtcgggtt ctcccgggcg aacgtcgggt acgcccaccc cttcttccat gcggccaagc
    32821 ggcggaactg cttctttggc gacaccgaga tcgagacctt tgacgggcgg gagtggtgca
    32881 ccaggccgat caggcagatc gtcgccgaga actttgacct ctcgaggccc gggatcgacc
    32941 ggctcgggac atactactct gacccgtcct ccacccttct cgtgcgcact gtcgaccggg
    33001 aggggaagcc ccacctgagg cgggtgacgt cggtctctct ccacaggtcc cccgaggccc
    33061 tgatccgctt cgagacccgc ggcggccggg agatcgcggt gacccccgac cacgcgatgc
    33121 tcgtctggga catctgctcg ctccggaaga tccgggcggt cgaactgaag gagggggacc
    33181 cggtcccggt gatggtgggg gcaaacgtcc tcaccgacca catcatccgc cgggaggtcg
    33241 tcccgtcgcc ggacacgcgg gtattctgcc tgacggtggc agaggaccac acggtgctcg
    33301 caaacgggat cttcaccggg cagtgcgacg gggatgagga ctgcatcatg ctcctcctcg
    33361 acgggctgat caacttctcg cgctcgttcc ttcccgagac gcgggggggc tcgatggacg
    33421 ccccgctggt cctgaccacc cggattgacc cggctgagat cgacaaggag agccacaacc
    33481 tggacgtcgg gccgggctac cccctggagc tctacctggc aaccctcaat tacgcccacc
    33541 caaaggaggt ggaggggctc gtcgaccggg taggccgccg cctcgggacc ccggcccaga
    33601 tcgaggggtt ctctttcacg cacgacacga cggatatctc cgccggccct ctcgagtcca
    33661 cctacaccca gctcaagagc atgatcgaga agcttgacgc agaactggct cttgcaggga
    33721 agatcagggc cgtggacgag gacgacgtgg ccgaacgggt cctcaccacc cacttcatcc
    33781 gggacctcca ggggaacctc tcggccttct cgaagcagaa gttccggtgc gtgaagtgca
    33841 acacgagcta ccggcgcatg ccgcttgcgg gcaagtgcaa ccgctgcggg gggaacatca
    33901 tccccaccgt gcacgagggt tcggtcaaga agtacctgga gatgtcccgg gacatctgca
    33961 ggaaatacaa ggtgtcggaa tacaccaggc agagggtgca ggtgctcgac atggcgatcg
    34021 agtccacctt cggccaggag aagagccagc agatgggcct cgcggacttc atgtaaggag
    34081 tatcccgcac atgtcgtcgg gatcagggca ggtgacaacc ggcatggacg ccttatccac
    34141 gtccggactc ctggatattc tcaagcatca cacaccttcg ggccatggtt cgatccaggc
    34201 aatccgcaag gatttttcca tgctatactg cagtttccag catgacaggc atgcccacca
    34261 acagccaatc ctgttgtccg ggaccatacc cgcatgctgg atatccgcat tgcaatccag
    34321 gccagaccgg acagtactct tcttccatgg aggtggtttc tctctgggtt cgacagccga
    34381 tcatctcggc ctctgcaccc ggatcgcaga agaggcagag gctcgggtga tcagtatcga
    34441 ttaccgcctt gcacccgaac acccctttcc catagcaatc gaagattgca tcaatgccta
    34501 tttatcactc ataggcaagg gaattcccgc aaaacggata attctcgcag gcatttctgc
    34561 cggggggaca ctcgcgctct ccacattatt gcgtgcacgt gagagagatc tcccccttcc
    34621 cgctgctgcg gtctgcatgt cacctgcagt tgacatgaat ttccgcggga attctattta
    34681 taccaaccag gagaccgact gggtgactcc tgggcgattg gagtacatac gcgaagtata
    34741 cctgaacggc cacaaccctt ctgatccgct agcctcacca attttcgcgg atctttcggg
    34801 tcttccgcct ctttatatcc aggccggggg aaacgaggtg ttaatcgatg gaatacaggc
    34861 atttgtggcc agagcggagg atgcgaaaat cgaggtgcag tttgactgct acgatggcat
    34921 gttccattgc tggcaggtat ttgccgatgt agtgcaggaa ggagtagttg ccattacccg
    34981 aatcggggag tatatccaat cgcgtttgat tcaaaactcc tgaagtttga catttttctc
    35041 acaagttttt gataagtgca tgcccttttc catgtaccct ttttttcagc aatacgggtc
    35101 ctcctctcac aattccaggc tcaccttgcg gaggagggga agagcaattc tcatcatcgt
    35161 ccagggtcta tatctctgga gaatgtacga cctgtcccgt ctcaggcagg agttcccggt
    35221 cctctcccgc tgcacctacc ttgactccgc ttccacgagc cagaccccga aaagggcggt
    35281 cgaagcgatg tgcgcctatt tctatgaata cgccgcaaac cacgggaggg gttcccaccg
    35341 cctggcgaga aggaccaccg aggagtatga acaggcccgc gaggcgctgg gcgggttcct
    35401 cggggcggac cccgggcacc tcgtctttgc gaagaacgcg acggatgcga tcaacatggt
    35461 cgcccacggt acctgctggg agccggggga cgaggtggtc acaacggcgc ttgagcacca
    35521 cgcaaacctc ctcccgtgga tggcgctcca gggcaggggg gtccgggtga aggtcctccc
    35581 tcagcgggac gggatggtcg atcccgccga cctccaggag gcgctcaccc ctcgcacgag
    35641 gctcgttgca gtcacccacg tcacaaacgt gctcgggagt gtccagcctg tggaggagat
    35701 ggcttcgatc gcgcacgatg ccggggtgcg catcctcctc gatgcggccc agtcggcggg
    35761 gcacatgccg ctctccgtgg gcgggttcga cttcgtggcc atccccgggc acaagggcct
    35821 gctcggcccc caggggacgg gggcgctctt tgtcgccgat tactccgacc tctcggtcac
    35881 ctgccacggc ggtggcatcg tcagtgaggt ctcgctctcg ggcttcaccc tcctcccccc
    35941 gccggcacgg ttcgagcccg ggaccccgaa catccctggg gtaatcggcc tgggcgcggc
    36001 aatcgggctc gtggaggagc ttggggtcgg cgagatccac caccacgaga ccagtctggg
    36061 gatacaggcc cacgaaggcc tctcatccat tgaaggggtg gccgtctaca gcccccgcgg
    36121 gtcgccggtg gtctcgttca acatcccggg gatgagccct gacgactgtg cggccgcact
    36181 cgaccggagg aagggaatct gcgtccggag cgggttgcac tgcgcccacc ccctcgcgtc
    36241 atcgcttcac ccccttggaa cggtcagggc ctcgatgggg tgctatacca cgggtgaaga
    36301 ggtggccatg ctcatcgaaa ccgtggagga gattgccagg tcccggtaac ggtggatgaa
    36361 cggaagggct ggggggcaaa aaagttattc gggtttcttc tcttcgggcc gcgttccttt
    36421 cctccccttc caccaacggt aggctgcgta ggcgaccagg ccgaggacga ccagcgggat
    36481 gatggagatc aggatgatca cgagtgcggc tgcaaccccg aggaacccgg cgatgccctc
    36541 gttgatcacc gaggtgatcg atggacctcc cccggcgccc acgggctcgg gctcccggaa
    36601 ggagacggtg atcgtcgcgt agtcgatccg gctgtccagg tacttcagtc tcccgtctat
    36661 gcggtcgatc tccacctgga cgcgctcgat ctgctcctgg acctcgagga tctcggagac
    36721 gttctctgcc ttctccatga tccgctggta ctggacgagc tggtttgcga gcgccgcacg
    36781 ccgggcctgg aggtcgacat actcttccgt aacgtcctct gcctggaccg actgggactg
    36841 gagggtgccg agtgcggcaa cagtggcgag cgccttctca aaggaggcag ccgggacccg
    36901 catgaccagg gtgccagaga ggcggtcccc gcggtagcgc tcgagcgaga gtgacccgac
    36961 atacccctcg ttttcggccg cgatggactg cagggcatcc ctcgtggcca tcacgtccct
    37021 cacttcgagg tcgatcctgg cagtcctgat gatcttctgc tcgtcgcctg cccaggcacc
    37081 ggcgacgggt gcaggggatg gcgtggagta gggcgcgtag ttatagtcaa cacccatcgc
    37141 cccctcgctg gccttctggg aggacaatgc tcccgggata tcgggggata cgcaccccgc
    37201 actccagagc ccccccgcaa ggattgccag gagaaggaga agggtgaata cttctcttct
    37261 cttcatacaa gtccctcccc tgcgaagaag aaaaatgatt ccgtgagtgc gaatcgtttc
    37321 gcagtctttt tcccctgtca gcggtcgggg gtttgcaacc tggtgaatat ttatcccggc
    37381 tgcccaccct tgataatagt atatccattc ccgaccgcga gtgtgactga gaggccaaaa
    37441 gtgaccgact caagatcggt tctcgcagga gttcgcaggt tcgaatcctg ccactcgcat
    37501 ccggttttcc gggagtcgtg gctgtcccgg gaactctttc tgatccgcgg ctgcaccaag
    37561 gcgcctccca cacccacccg gagctgtccc cccggacaca tccttatctc acccgaagag
    37621 aaaccgtgta accgcatgtt caacgcgctt gacgtggacc gctgggtggc ggaacgccgt
    37681 tcgagcctcg acgaggcaaa ggaatcggtc gcggcgatca tccaggctgt ccgcaaaggg
    37741 ggtgacgggg cgctgcttgc catggcgagg aagcacgagc cggacatcac atctgtccgg
    37801 gtcaccgaag aggagattgc ggcggcgtat gacgaggtgg aagacaggct cgtggagagc
    37861 ctggtcgagg ccgaggcgcg gatcaccagg ttccacgagc tgcagaagga gcggagcctc
    37921 tggctcgaag aggtggagcc cgggatcgtg ctcggggtca agaccacgcc cctcgaccgc
    37981 gtgggccttt acgtgccggg caggagggcg gcatacccct cgaccgccct gatgaacgcg
    38041 gtcccggcaa aggtcgcggg ggtcccggag atgtgcgcct gcactcctcc gcccgtcctc
    38101 cccctcaccc tcgttgcgct cgacatcgcc ggagtcggcg aggtctaccg tatcggcggc
    38161 gcccaggcgg tcgcggcgat ggcgctcggg acagagacca tccggcctgt ccagaagatc
    38221 gtcgggccgg gcaacgtgta cgtgacggcc gcgaagatga tgctcaggga gtacgccgag
    38281 atcgacttcc cggcgggacc aagcgaaata gggatcatcg cggacgggac tgcaaacccg
    38341 cggttcatcg cgatcgacat cctggcccag gcggagcacg acccggacgc agcctgcgtc
    38401 ctcgtcacca ccgagaaaac gctcgcggac cgggtgaggg ccgagatagg aaggctcctc
    38461 gaaaaggcgc ccaggcggga gatcatcgac aaggcgctcg ggcattcggg atacgtcatc
    38521 gtgcccacca tggaggaggc ggtctccggt atgaacgaga tcgcacccga gcacctctcg
    38581 atccaggtcg ccgatgcgct cacggtcctc aacgcggtca ggcacgccgg gtccatcttc
    38641 gtcgggccgt atacccctgt tgcggccggt gactatgcat ctgggacaaa ccacgtcctg
    38701 cctacggcag gatacgcgaa gacctactct gggctgaacg tctcgcactt ctgcaagacc
    38761 tcgacggtcc agatcatcga ccgggagggc cttgacgcca tcggcgatgt catcgaggca
    38821 attgccgacg ccgaggggct caatgcccat gccgagtctg tgcgcatcag gcgctcattc
    38881 aactgaataa cccctcttct ttcttgcgtg cgcacctctt tttcggacac cggaccggga
    38941 gggagtcggg aatgtataag gacccaatac aatctccgat acctgccttg tcactgaagt
    39001 gtaattgggg cccgtgatcc cgaaaagcgt gcggatcgtc cggaactttc tgcaggcacc
    39061 cggatgggga tatccctgtc gaagaaccgc tacttcctgt aatacgactg ctcgcgctcc
    39121 cgtatctcct ggaggcccac caggcggttg aactcgtcga accctaccag gtgcccggga
    39181 tacgatgcag aggacccggt ccgcttcagt tccgagaaga cgtgccggac tgcgtgcgtg
    39241 atagcatacg tgcaggagac agggtctgcg acgacggcgt acccgatctc atcaagttcg
    39301 ggagcggaga ggaccggggt ctttccgcca gggagcatgt ttgcaaacgc cgggccattg
    39361 acttccctgc atatccgccg catctgctcc actgactcgg gggcctcgac gaacgtgagg
    39421 tccgcgccgg cctcatagta gaggttcgcc ctctcgattg catcctcgat gccgttcacg
    39481 gcaatcgcat cggtccttgc catgatcacg aggtccgggt ccacctttgc atcgactgcg
    39541 gccctgacct tcgctgccat ctcttctgcc gggatgagtg cttttccctc catgtgaccg
    39601 caccgcttcg gggagacctg gtcctcgatg aagactcccc cgacgcccgc tttctcatac
    39661 agggacacgg tacggacaac gttcgtcacg ttcccgtatc ccgtgtcgcc atccgcaaag
    39721 accgggatcg tcgtcgcgtc cacaatcctc gaggcgcagt cgaccatctc agtgagcgtg
    39781 agcagcccga cgtcgggcct gcccaggagc gcggcggagt tggcgtagcc cgccgagaat
    39841 atggcggaga accccgcctg ctcggcgatt ctcgcgcaga gggggtcgtg gacaacgggg
    39901 atgacaagaa tagggtctgc tgcgatcagt tcgcggaggc gggtgggggc tctcattggt
    39961 tttcctcacc aggagtaaaa accggccggg cttattcttt tcgccggaat ggaccgtgtg
    40021 ggccttgccg ggcctgcacc atcttcatcc ttattatgca tcccctcacc taactctaca
    40081 ggggattttt gaaaatggcg atcgactggt acatggactt cgtggataag gggtatgaac
    40141 ccgcaaagga cgagctggac gcactctttt acttcgagcc ggcagccggg atcagcagcg
    40201 aggaggcggc agggagaatc gcgtccgaga gctcgaccgg gacctggacc acgctccacg
    40261 cgctcccgcc caggatgagg gacctccagg ccacggtgta cgagatcgac gggagatacg
    40321 tgcggatcgc gtatccacaa ggcctctggg aggaggggaa cgccgcccag ctcctgagcg
    40381 ggattgcagg gaacgtcttc ggcatgaagg caatcaagaa cctgaggctc atcgacgtga
    40441 gtcttcccca ggggtatatc cggcacttca aaggcccgca cttcgggatg gagggcatcc
    40501 ggaagatgat gcggatagag ggccgcccgc tgaccggcgc ggtcccgaag cccaaggtcg
    40561 gtttttccgc acgggagcat gccgagatcg ggtacgagac ctggatgggc ggctttgact
    40621 ttgtaaaaga cgacgagaac ctcacctcga catccttcaa ccggttcgag gaccgcgtcc
    40681 gggagatgac caggatgcgt gatcgggccg agcaggccac cggtgaggag aagtccgcct
    40741 tcctcaacat cacgggagag acggagacga tgaaagagcg tgcgaagatg ctcgcggaca
    40801 gcgggtggaa ttacgcgatg atcgacgtgg tggtggcagg gactgccgcg gtctcgacgc
    40861 tccgtgacta ctgctcggac cttggcctcg ctatccacgc ccaccgggcg atgcacgcgt
    40921 cattcgacag gaacccaaag cacgggatca ccatgcagtt cctggcaaag atgatgcgcc
    40981 tgatcggcgt ctcccagatc cataccggga ccgcggtggg caagctcacc gggtcaaaga
    41041 aggaggcccg tatcctcgcc gaagtgctcc gggagcgccg cgtgaaggag gtccgccatg
    41101 ccctcctctc ccaggaatgg ggcccgataa agagtgcatt cccggtatcc tccgggggcc
    41161 tgcatcccgg cctcgtcccc gatgtcctcg acatctacgg gacagacctc gtgctcctcg
    41221 tcagcggggg tatccacggg catccgaagg ggacccgtgc aggggcgatc gcggccatga
    41281 aggccatcga ggcatgggag gaggggatga gcctcgacga gaaggccaaa tcatcgcccg
    41341 aacttgctca gtccctggag aaatggggat actacaggcc caaatgagtg cagtacggaa
    41401 atgacggcag cgagggatcc ggcccccgct cctggatgga attcccccgg aacaggcaat
    41461 cggggatcat tggccggtct tcccaaactc ttttgcatct cccgacatag gttcattgca
    41521 tgcgcccgga ataccgtgaa atcttttctc cgcacctctc tctctggcag ttcctcgccg
    41581 ccctcgggtt tggcatcctg gcagcgctca ccctcttctc ggacctcgcg atcccggtgg
    41641 gccatgggct ccttgcagag ccgagtgaag tgtttgtcac gctcggggcg gcattctcgg
    41701 gcccgttcgg gggaatcatc gtcggcctcc tgcagggggt ggtgtacgcc ccggagagaa
    41761 acatccccgc ccacatgctg gccgggttcc tgtggggggc ctggtacgtg gtgctgtacc
    41821 ggtggagcct cgggaaaggc aggtggacga gggcagccct ctgggcggcg agcatccccg
    41881 tctattatta tgtcctgctg ctcccgctgc atatcgcgat cgcctcgttc accctgggga
    41941 tccccctgct ccccctctac gcggaggctg cagggggcgt tctccccgag atggcgttta
    42001 ccctcctcgt gacatccgcg gtgatggctg cactccccga gagatgggcc gctcccctcg
    42061 attgaggtca gacgagggcc cggatcaccc gggcgaggcg ctccatgccc accctgatcc
    42121 gctcgccgtc catgctcgag aagttgaggc ggatggtgga atcgcccccg cccgatacgt
    42181 agaagggggt tccgggaagg accgcgaccc cgcctttgag cccctcctca aagaccttca
    42241 tcgagtcgag ccctttcggg agggtggcga gcaggaacat cccgccttcg ggctccgtgt
    42301 gatacatccc gggtgaaaac aggtcatcca ggaggtcgca catgagccgg cagttctgcc
    42361 cgtacacggc agaaattcgg cggacatgat catcgaggtc gcatgtctcg aggtaccgcg
    42421 aaaggacttt ctggcagagg aagttcgagt gaaggtcggc agcctgcttg gcacagttga
    42481 actgcgcgag aatctctttt ggagcacata tccacccgat gcgcatccct ggggcgatga
    42541 ccttcgagaa ggagcccgag aggatgcagc agtcgggagc gagggctttc acgggcttcc
    42601 tgggacggcc gtcaaagaag agctccccga acgcgtcgtc ctcgtaaaat acggtctccg
    42661 acccttccag gacttcggcc acggcctttc gcctctctgc cgagtaggtc tggcccgagg
    42721 ggttctggga gttcggaatg ccgtaaaaga acttcgggcg gacctcccgg atgacccgac
    42781 tgaactcgtc gatatccggg ccctctgcgc cgaacgcgac atcgtaaaag gtcggttcat
    42841 aaagggagaa ggcctcgatc gccccgaggt acccgggccg ctccattgcg accgggtcgc
    42901 ccctgtcaag aaagatcttt gccacgaggt cgaggcactg ctgggatccg ttcacgacct
    42961 ggatctctga tgcgggaatg tcgaggccga ggcgttgccg gtatcgccgg gagatgaact
    43021 cccgcagggg gaggtagccg tcggtggtgg agtactggag ggcttctctc ccctcctcct
    43081 cgaagacgcg acgggatgcg tccgcgagac cctccacgtc gatgaaggcg gagctcggca
    43141 gccccccggc aaaggagatg atgcgggggt cgctggagac cgcaaacaac tttccgagaa
    43201 acgatggcgg tgcacagtgc atccggccgg cgaatcggta ctgcatgacg gaaacttccc
    43261 ctgattctac aggtacttgg tggcgggaag gaaaaagtgt gaccctttgg cccgggatga
    43321 gggcacctga aaggcccggg agcgtggggg tcatccggga acgaaacgtt ccgccaacgt
    43381 gagagcacta attattaaag ccattataat ggtgaatgcc atggtgaaga cgaccatcgc
    43441 gctatccccg gaaacacgcg atctcattag agacctgggg aacaaggggg agacatacga
    43501 tggtatcatc atccgtctcc tgaaggatgc gggctggaaa caccttgaca cacgctggaa
    43561 cgaaattctc gagaacgatg aattcatacc cctggatgag ctctgatgtt cagagaactg
    43621 gcatccagga cattccagag ggagttttaa aaaaattccg ttaaattctc aaaccaggat
    43681 caggaaaagc cagacatgcc tggaggaaga tccatatgct ccccgtgccc atgccgacat
    43741 caagtcatta aaagacacgc atccccagaa ataccggtta agagttagga attttcggat
    43801 tgtctataca ttcgggggca atgaggtgaa gatcctcgat attcttcatc gcaggcgagg
    43861 ctatttgggc caataacgcc tcgtttttga atgcgaagat cgggaaaaag aaggagtcac
    43921 ttccactggg catgatttca caaccgggaa tcaccacatg ccttattgac agatgaaacc
    43981 agtcggaaca gcgcgatgaa tgtccttgtt gtggggttca ggggaggggt gccctgatgc
    44041 gctggagcga gaccttcgcg ttcctcctca cccggcagtc gggatcttct gccgccttct
    44101 cgaggattgc ctcggcttcc cgcgaacgca tgtttccgag tgcgcaggtg gcggcgttcc
    44161 gcaggatgtc gtctccgtca cgcatgacgg agcgaagcac cgggatcaag ttgggacagt
    44221 cgagggcccg tatcccggcc gccgcagcct cccggatgta ccacttctcg atacggagcc
    44281 tcgaaaagag tatggagagg tcttcagcgc tcgcaaggct cccgatgcgc cgcaccgcgg
    44341 cctggcggat gctccagtca tggctgtaga ggtcttttac gatggcgccg accgtatcgg
    44401 catctgcacg ggttccggtc atcctgcgta ctcttttgtg cgcaaacaat atatattatt
    44461 ggatggaagt ttccttcttt aaccctggct ctgccgccgc ccacatccag cacttcccgg
    44521 catacaaagg gccacgctct gtcctcctcg cctggatccc gtgccgctca ggcaggtaaa
    44581 tccgggaaaa ttctataagc tcacacttca aaaaatgaaa tatttatcgc ggacacgagg
    44641 gcaggagccg aggagttgat tcgctgatcc agaggaagac cgacgagga
//
LOCUS       DAOQYR010000008        42791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_1009_length_42791_cov_8.175847, whole genome shotgun sequence.
ACCESSION   DAOQYR010000008 DAOQYR010000000
VERSION     DAOQYR010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 42791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42791
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1009_length_42791_cov_8.175847"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..779)
                     /locus_tag="PKM87_01860"
     CDS             complement(222..779)
                     /locus_tag="PKM87_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNQ28840.1"
                     /translation="MHVTHPQVYPPQEDTYLLLEAALQEAKPGDFVLEVGCGSGHIAG
                     ILAGKNRVLASDINPHAVRETYARKVDAVRADLLAGIRGPFDLVIFNPPYLPTLPDER
                     IDDWLELALDGGRDGREVIGRFAVQVGRVLAPGGRVLLLLSSLTGLERIRGIFSEQGF
                     DCEIVGEGRVEGEILYAIRCIPHRG"
     gene            complement(770..1540)
                     /gene="rsmA"
                     /locus_tag="PKM87_01865"
     CDS             complement(770..1540)
                     /gene="rsmA"
                     /locus_tag="PKM87_01865"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNQ28841.1"
                     /translation="MSARNDQHFLVDRNAVARIAGAIEVTGRTVLEIGPGRGILTRAL
                     LGRGARVNAVEIDPELVECLTLEFEEEIRAGRLTLTRGDAVRCTLPQFDVVVSNLPYS
                     ASSKITFRLLERGFDEAVLMFQSEFADRMLAHPGTPECGRLSIMVQTYCAAGRLFDLP
                     PRAFSPQPRVRSTVVHLIPRDPFFPIHDRRLYADVVRALFSHRRKTVRNCLKGAGGMI
                     APQQVERMLAGLPDEILSSRPEALYLEDFATIANLGCT"
     gene            complement(1537..2097)
                     /locus_tag="PKM87_01870"
     CDS             complement(1537..2097)
                     /locus_tag="PKM87_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF655 domain-containing protein"
                     /protein_id="HNQ28842.1"
                     /translation="MKTEKKEVNAIVLDVLQKGHPDDPRPVFKREPLVQGVGLDQFKL
                     LEMVPKTLEIQIHESVYIGDGEREKIERVKRRISYDDLTQTARLELPFAVERIVKEKE
                     PEFVQFFNKSISISPKLHMLHLLPGIGKKLMWEILQEREKKPFESYSEISQRIKSIPH
                     PEKMIIARVLEELQDPTVKYHLFTSR"
     gene            complement(2106..2456)
                     /locus_tag="PKM87_01875"
     CDS             complement(2106..2456)
                     /locus_tag="PKM87_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase Rpb4 family protein"
                     /protein_id="HNQ28843.1"
                     /translation="MKVKGIIREERITLPELREVLHQVETERIDAGKEMSYELRKSIE
                     HANQLSKASSEKSRLLVSELLKMEKMKPDIAFRIANVMPRTRDELRAIYAKEKFTLQT
                     DELDTIIEIVRSHS"
     gene            complement(2491..2781)
                     /locus_tag="PKM87_01880"
     CDS             complement(2491..2781)
                     /locus_tag="PKM87_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21e"
                     /protein_id="HNQ28844.1"
                     /translation="MAHHNGPRKKTRYKFKKDLRKRGVVPVTSLIQKFDLGQKVHVVC
                     EPSIQKGMPHRRFHGMTGTVVGQRGRAWLLSIRDGEKEKIVIARPQHLKAQR"
     gene            complement(2787..4016)
                     /locus_tag="PKM87_01885"
     CDS             complement(2787..4016)
                     /locus_tag="PKM87_01885"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(54/55) synthase Pus10"
                     /protein_id="HNQ28845.1"
                     /translation="MVILEDAQKILSYGEICDHCLGRFFGKRSFGLTNEERGRALRIA
                     LSLEDNTPYQSPGPCWICGDLFSQVEVWADRVIAATGGTEFERFVIGTRVPPLMAESE
                     EIVWSDLSLTHAEPLKSEMNREVGKAVARKTGRIADPKQPEVVAILNLPEECVEVQIN
                     PIFFYGRYRKLERGIPQTHWHCRACQGQGCEACNFTGKQYLDSVEELIGRPVMRLFLA
                     ENAILHGSGREDIDALMVGTGRPFVMEVVAPRVRNPDLHTIEEAVNREAGGRVSVTLE
                     RWSSRDEVEIIKSTKGYKKYRILVEIDGPYSYEELQSAIRALKGAKINQRTPLRVAHR
                     RADRVRERRVLDIGIAGEADARVILEVTGEAGLYIKELVSGDQGRTEPSLSGLLGRSA
                     RVCSLDVVQVQGHNEGE"
     gene            complement(4010..4585)
                     /gene="trmY"
                     /locus_tag="PKM87_01890"
     CDS             complement(4010..4585)
                     /gene="trmY"
                     /locus_tag="PKM87_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (pseudouridine(54)-N(1))-methyltransferase
                     TrmY"
                     /protein_id="HNQ28846.1"
                     /translation="MRRVAVVGHLARTAGDFNLSDMPGGAGRMDVLCRCVNSAFFLSH
                     EFRRDVECYLVLLGEPDAPKTVRFRGENVRYLSPDERSAGSLIKKALAVPAGTGFRES
                     TPGVEVRRGGLSELLAEYSFSVLEESGTDARGMAGLPDSYLLSDHLDFTAPERELLEG
                     LERISVGPRSLHADHAITVLWNEIDRREAGW"
     gene            complement(4595..5914)
                     /locus_tag="PKM87_01895"
     CDS             complement(4595..5914)
                     /locus_tag="PKM87_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein Srp54"
                     /protein_id="HNQ28847.1"
                     /translation="MLDRLGTSLKDALKKLAGKTVVDRAAVEELVKDLQRALIQADVN
                     VKMVMQLSQAIKTRSLEEQPPKGMTVREHVLRIVYQELVRLVGSAGEARLEPQVILMA
                     GLQGSGKTTTTAKLARYFQRKGLRVGVICADTFRPGAFEQLNTLCSRINVSCFGDPKE
                     KDAIRIVREGLPAMSGNEVVIIDTQGRHALEAELIQEIIDIQKIASARHRWLVIDAAL
                     GQQAAEQARRFHEAINIDGVIITKMDGTAKGGGALSAVAETRSGIVFIGSGETSDDLE
                     RFEPDGFISRLLGMGDLKALVERAEETMAGQEVDVNAMLRGKFSLRDMYQQLEAVNRM
                     GPLKQIMSMLPLGNMEIPQDAYDVTSTKMQRYRIIMDSMTSTELDDPSVMGSSRIYRI
                     ATGAGASPEEVRELLRYYKMMKKALKGVRGGQGKFNMQRMMKKFGGM"
     gene            complement(5914..6936)
                     /gene="ftsY"
                     /locus_tag="PKM87_01900"
     CDS             complement(5914..6936)
                     /gene="ftsY"
                     /locus_tag="PKM87_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNQ28848.1"
                     /translation="MFEGLRARLQKVQGTLEKNIAKTASPADTAAVPEECEGAVSGKD
                     PSLIAKVKVLITDREFFIAEKDIEEPLFELEMILLENDVALPVTDEILTRVRADLTGK
                     RRKIGESVDEIVVTALKGALLGVLGEGFDLLAYIRTHEKPVKILFTGVNGTGKTTTVA
                     KVASYLKKNGCSVVIGAGDTFRAGAIEQIDVHAGRIGIKVIQHQAGADPSAVLYDTVQ
                     YAEAHRVDAVLADTAGRFHNRANLMNQLEKIRRVMKPDLVVYVDEAVAGNDAVVRAFE
                     FEKTVGADAVVLTKADMDSKGGAAISIAHTIGKPVMFLGVGQGYDDIIPFSPTRVVDE
                     LLGVEA"
     gene            complement(6959..7429)
                     /gene="pfdA"
                     /locus_tag="PKM87_01905"
     CDS             complement(6959..7429)
                     /gene="pfdA"
                     /locus_tag="PKM87_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit alpha"
                     /protein_id="HNQ28849.1"
                     /translation="MVSKVEGIDPRELQSLQLYLNEYRQQAEIFSQQLALLEEGRVEA
                     LAAIDAITGIKESSEDTVLLQIGGGVSVRARIEEPERVLLNIGADVVVEQSTEAAVEY
                     LKDRLTEMDASAKKVMETLDQLRYQMNEIARRLEAGYQQYQMQQARPVQGEELG"
     gene            complement(7419..7610)
                     /gene="rpl18a"
                     /locus_tag="PKM87_01910"
     CDS             complement(7419..7610)
                     /gene="rpl18a"
                     /locus_tag="PKM87_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18Ae"
                     /protein_id="HNQ28850.1"
                     /translation="MADQKYEVKGDFRMGDEWRPYAKVVEAPNAAQAKERTFALIGSK
                     HRLKRSYITVQSITLLSGE"
     gene            complement(7618..8280)
                     /locus_tag="PKM87_01915"
     CDS             complement(7618..8280)
                     /locus_tag="PKM87_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-6"
                     /protein_id="HNQ28851.1"
                     /translation="MDRTISIAGDSNIGVFTRVIGGLAVVPPVAPAEFQEAIIELLDV
                     EIIPTTIQGSEIVGSLLAGNRRGFVVSGLAHDREIALLEEYGQVYLLEHTMNAAGNVI
                     MANDSFAAVHPDMPREMAGEVGEFLGVRVFPLTLGGIKTVGMAGVATNKGVLVHPRSS
                     RAEIQVLEQAAGVPIGTGSVNCGVGLVGTGLLVNGHGYATGLETTGFELGRVEDVFGF
                     LE"
     gene            complement(8282..8545)
                     /locus_tag="PKM87_01920"
     CDS             complement(8282..8545)
                     /locus_tag="PKM87_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31e"
                     /protein_id="HNQ28852.1"
                     /translation="MADKMEEQIYIIPLRDVKRAPRWKRSNTAIKDIRKFLAKHMKSQ
                     DVKLDRTINERVWDRGAEKPPSRIRIRAMKFDDGQVQAELAEE"
     gene            complement(8551..8703)
                     /locus_tag="PKM87_01925"
     CDS             complement(8551..8703)
                     /locus_tag="PKM87_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L39e"
                     /protein_id="HNQ28853.1"
                     /translation="MSRSTKGYKVRLAKACEQNRRVPAWVMMRTKRQVSTHPRRRNWR
                     RSTLKV"
     gene            complement(8712..9308)
                     /locus_tag="PKM87_01930"
     CDS             complement(8712..9308)
                     /locus_tag="PKM87_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha hydrolase"
                     /protein_id="HNQ28854.1"
                     /translation="MKAGVLFSGGKDSSLAAVLLSRDYSVELNTFVFSHQRSLAAVER
                     AALALGFPYRRRTFREDLLGRAVEMVLSSGYPNAAIQMVHKEALCILSTEYRVVADGT
                     RFDDRVPMLSRDEVQSLQDTSGCSYLRPLLGYPRREVDRIAARHLEIAFGETGRIENG
                     DYEAEIREEVRARGEDSGKYFPLHHEQSLVTGTITISG"
     gene            complement(9305..9646)
                     /locus_tag="PKM87_01935"
     CDS             complement(9305..9646)
                     /locus_tag="PKM87_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNQ28855.1"
                     /translation="MGDDELAEIRRRRLAQMQQQSIDQQVMQEEMERQKQVEAQIHAV
                     LMQILEPEARERLNTIKLTRPDFARAVEQQLVMLAQSGRLKQKITDTQLKSLLQQLQP
                     AKKDFRITRKG"
     gene            complement(9657..10094)
                     /locus_tag="PKM87_01940"
     CDS             complement(9657..10094)
                     /locus_tag="PKM87_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19e"
                     /protein_id="HNQ28856.1"
                     /translation="MTTVYDVPADRFIRTLAEELKKKPEITPPAWAAFAKTGVHKEMP
                     PQDPEWWFVRVASILRRVYIEGPIGVQRMRSIYGGKKDRGSRPSQFRKGSGSILRKSL
                     QQLESAGLIQHDKTGRRVSPSGISYMDGLADRIAKEGFAQVQK"
     gene            complement(10216..10461)
                     /locus_tag="PKM87_01945"
     CDS             complement(10216..10461)
                     /locus_tag="PKM87_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HNQ28857.1"
                     /translation="MRDERRRSGMQEMKATVWIGKQGLTPTVVEEVVQQLKKRHQVKV
                     KWLRNTEVDPAELARSAGAVLQEVRGRTAVLAERKAL"
     gene            complement(10458..10772)
                     /locus_tag="PKM87_01950"
     CDS             complement(10458..10772)
                     /locus_tag="PKM87_01950"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component 4"
                     /protein_id="HNQ28858.1"
                     /translation="MARKVPRSDTRRIARERIEILFSRAGECFHADPESSNRYVALAR
                     KISMKHRVRLEPCFRRQFCHHCGNFLVPGENVRIRIRRGMVVVTCLSCGKHMRFPVRR
                     QR"
     gene            complement(10832..11452)
                     /gene="purN"
                     /locus_tag="PKM87_01955"
     CDS             complement(10832..11452)
                     /gene="purN"
                     /locus_tag="PKM87_01955"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNQ28859.1"
                     /translation="MKRVAVLASGRGSNFQALIDAIRDGAIQAVCVGLVTDNPRAHAI
                     SRAEDAGIPVHVVDYSRFPGKEEYEAALLTAMKGLRSDLFVLAGYMRILGPEIVREFS
                     GKMINIHPALLPSFAGLHAQRQAIEYGVKVSGCTVHLVDEGMDTGPIIVQACVPVLDD
                     DDEESLAGRILAEEHRCLPMAVKLFCEGRLAVDGRRVVLREGGPQG"
     gene            complement(11449..12729)
                     /locus_tag="PKM87_01960"
     CDS             complement(11449..12729)
                     /locus_tag="PKM87_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNQ28860.1"
                     /translation="MIEDFLLLLLFILCLVLSGFFSASEVALVSITRAKVRTLANEHR
                     PGSDALATLKEHPNRFLITILIGNNIVNVAAAAIATAVTISIFGDIGVGIATGVVVIL
                     LLLFGEIGPKIYAARNTERFALAVARPIYYLSRILSPLVWAIEMVAVRRTGEGGFAEP
                     AVTEEEIREWIEVGKEEGAIEQEEREMLYSVLEFGDTTAREIMTPRVDVALIDDQSTL
                     EDALHFFNETGFSRIPVYHEHMDNIIGVLNVKDVISSQISGKKNIPIKDLMYDPFFVP
                     ESKKIDDLLKELQLRKVQIAVVLDEYSSFVGIVTVEDILEELVGDILDEFDREEPEIQ
                     KISDGVYLVDAKVWVEDLNDELDISLPVSEAYESLGGLIIERLGHIPHPGESVYIQES
                     HISLVVTQMLSRRIVKVKLILHHVGEESAEETRR"
     gene            complement(12821..13642)
                     /locus_tag="PKM87_01965"
     CDS             complement(12821..13642)
                     /locus_tag="PKM87_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNQ28861.1"
                     /translation="MVHFAVSSMFFHEYPLEEIFSYIVEAGFDSVEFWVETPGYWMAG
                     QPEEELMKNLGAFSQLVSPTIHAPVLDLNPTSINPAVAEVSVRYTVEAVCLAARVGSG
                     VVTVHPGRRTAKRPPSAPDYERFARYIRALEEAATGFGVTVAMENMERKINSLLCTPE
                     GMKELLDREPWLGFTLDISHAMGAGPGEAQRYIELCKDRIVNVHVGRAESGKMHLPID
                     GDEEIADILGQLSASGYDGNLTLEIEDRNFSHDLSSEEKILVLSRELAFMRDCFV"
     gene            13681..14928
                     /gene="xseA"
                     /locus_tag="PKM87_01970"
     CDS             13681..14928
                     /gene="xseA"
                     /locus_tag="PKM87_01970"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNQ28862.1"
                     /translation="MTWYQKGISDERGGHTTEVLSVSQVSSLVEALLDDTRLQDIWVR
                     GEITNLKLHGSGHCYFSLGEKSRDHSAVLSCVMWRNDARRVECEFRDGMDVVVYGSIG
                     HYAPQGRYQLYVREVRPAGRGEKHLMVEKWKVQLTSEGCFDPQKKRPLPAYPLKVGVV
                     TSETGAVLQDIRNVVSRRYPLELVVSPTAVQGEGAHLEIARALQRVERDVDVIILARG
                     GGSFEDLFPFNYPEVVRAIVSCPVPVVSAIGHEVDITLADFAADVRAPTPSAAAELVV
                     PDRIALMDSLALFRKTLGNRLIVRLDRAGTDLSEVRERLSPRRVSQRVTAKREDVSVL
                     SGRMERAVLSRIALERERLSAAVGVLAARNPAQLLRRGYCILQKDGRVIHSADEIACG
                     DRLTLRLVDGRAVTRVEEVHHDS"
     gene            14918..15100
                     /gene="xseB"
                     /locus_tag="PKM87_01975"
     CDS             14918..15100
                     /gene="xseB"
                     /locus_tag="PKM87_01975"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNQ28863.1"
                     /translation="MTHKFEDLMKELKEILAKIEDNQTGLDEAIALYERGAVLVRQAE
                     ELLASAEMKITMLGRD"
     gene            complement(15115..15546)
                     /locus_tag="PKM87_01980"
     CDS             complement(15115..15546)
                     /locus_tag="PKM87_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF371 domain-containing protein"
                     /protein_id="HNQ28864.1"
                     /translation="MKREARAIIHCRGHPHVRAAHPSTLEITCEHEMTPNGDCIIAVG
                     ADCSARDLPEEFRILLCRDDASLHTVFSCGGMSVEVRGQGSSSMTLDHPADMVWRRSC
                     YVCGRTVAIGSDTTARMMPREFVEALRKGGEVVATLTVRVP"
     gene            complement(15692..16531)
                     /locus_tag="PKM87_01985"
     CDS             complement(15692..16531)
                     /locus_tag="PKM87_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28865.1"
                     /translation="MVLDSLKRACGLLLKNPFLWLPGLAAGALGAADLLLQYYLGAFL
                     ATRLWFIEALLIPFLLAGAYVQIRSGEKGAVAFVAGATAYYFRILLPSLVIVFAVLAT
                     VFLVVIPLTFMGLAGTALPFVVMGCTIPIIFFTFFFDTAAVFEDRRVFDCIRRSVEVV
                     MNRPGRVIVFYLVCLGIVVLVAIPLAVIWTGLLYEQLLPLATMDPSQVQAFSVSMFNE
                     MLGQEGILITAIFYLVAMMVAGNIICTFKAVFFRDTSDEIPADASPVQQGEFDEKGRW
                     YKY"
     gene            complement(16536..17126)
                     /locus_tag="PKM87_01990"
     CDS             complement(16536..17126)
                     /locus_tag="PKM87_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HNQ28866.1"
                     /translation="MSFVERNCYYFDAPGPANTVDCARFALERARELSIERVVVASTS
                     GDTALAFYRMLRGSGIKLVVVTHVVGFSRPGEWEFSPEAARTLKLEKIPLVTGTHVLS
                     GMERAFSRSPKVGGGSRTEAVAEALRRVISVGLKVAVECTLIAADQGVIPVDKEVIAV
                     GGTASGADTVCVVRPSHTASFFDLQVREIVAMPRNR"
     gene            17170..18339
                     /locus_tag="PKM87_01995"
     CDS             17170..18339
                     /locus_tag="PKM87_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNQ28867.1"
                     /translation="MITLALAGKPNCGKSTFFRAATMAHAEIANYPFTTIDANFGVAY
                     VRTECPCKSLGLQCGNCADGIRFVPVNLIDVAGLVPDAHKGKGLGNQFLDHLRQADAI
                     IHILDASGSTDTEGNPVETGSHDPREDIAFLQKEMTMWVYGILEKHWARLQRQAQSKA
                     YSIHRGISEVFTGLGITPEDVQDAERSVAIDLARAEPEGLVKFCAEIVAISKPMIPVG
                     NRVDMAPAPLLDTLRSAGITFATAAGELALRNAVSGGLISYHPGDPAFSIIKEESLSP
                     AQKAGLQTIAAVMKANGGTGVQQAIDRAVYELLGMIVVYPVEDEHKFSDSRGRILPDA
                     FLMKRGSTPRDLAFQVHTDIGKAFLYAVDARTKMRVKDTHELKPGDIIRIVSAAK"
     gene            18423..18743
                     /locus_tag="PKM87_02000"
     CDS             18423..18743
                     /locus_tag="PKM87_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28868.1"
                     /translation="MGGDVFQAQILRNFFDTITGTDRNLTRIYMCVVSLAKIRMEPPE
                     KFTFLLEQLRKSKQHKELSVDILDYMCDVAAQLEIAAVQTAFGVKEVGEIQQEFGSIS
                     MDSL"
     gene            complement(18825..19685)
                     /locus_tag="PKM87_02005"
     CDS             complement(18825..19685)
                     /locus_tag="PKM87_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28869.1"
                     /translation="MSANSSQNPDHVRKKREIQLALAVPLCAFGGLILAILLKEAGLI
                     ADAADFYWGCVAASLILAYLAYIKPRKDLVSLFAPAYALLAFVIPLETKASFLLQILY
                     AVSITLLVIRLHYRFSTPKTIAKEEDSMEKYLYNYIHRMTPLFRGIDPETAHEIASAV
                     LSFKFGLYAKTVTDAGKASSRIPKDGAGEVIGKALRILMDRALALEEARVGDYSPVRF
                     EAGDASWLPIVLKEEQIENQDTLTLDNALLLLYTVAYLQSPDDGQSLDEHQNFVLQIL
                     ESYREPLNLK"
     gene            19821..20999
                     /locus_tag="PKM87_02010"
     CDS             19821..20999
                     /locus_tag="PKM87_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HNQ28870.1"
                     /translation="MKVCIMCGGEGTRLRPLTFERPKPCIPIVNRPSIQHLVSHLSNL
                     GFREIVVTLGYMGDAIEDALGDGSLFGADITYVHEKIKLGTAGSVKNAQAYLEEGDFL
                     VVGGDHVTDLNLLDFYREHSRHQPPVSIGLISIDEPGEYGIAEIDADFQIKRFKEKPA
                     PGEIFSNLASTGIYVCRPEIFDEIPLGRKFDFARDLFPHLMESGHVLRAWLARGNWTD
                     VGNPASLRQAERWKLQEIPYTSISGDLTIRAGQIQGPVQLGTSFSMGVNSRIIGPVAI
                     GPGTAIGDRVLIGPYTSIGENCLVKSNAKIFSSSLYNRVVVGRNSTVSGSIIDNDTVI
                     GDDCSLENDTVIGPRVVFRNGVVVHSRTRIWPEVVVPDGTVVKEHLLNDEYAVKCEGS
                     "
     gene            complement(20996..21781)
                     /locus_tag="PKM87_02015"
     CDS             complement(20996..21781)
                     /locus_tag="PKM87_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNQ28871.1"
                     /translation="MDKKKVRDYMTYDVVTVDAQGTVREVMEKIQKTGHDGFPVVMGN
                     EVVGYVAARDLLFQPVTTPVRKVMSEHLIVADPDMSINDAARVIFRSGIQKLPVVDEQ
                     NRLLGIISNTDVIRSQIEHVSPEKVFNFITTLKTLHGISPELKREVVPIDQLLPTQSR
                     IYEDELEGRIYEIKKGLAEPLIVVRRPNKLILVDGHHRAIAARKMGIKTLDAYVIEVN
                     EDIELGMERTARAMNLRSLDDIQVLDYARHPLVAITHRLVRKG"
     gene            complement(21834..22304)
                     /locus_tag="PKM87_02020"
     CDS             complement(21834..22304)
                     /locus_tag="PKM87_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNQ28872.1"
                     /translation="MVYRRRYPFGWMMRDFEDLMGEMENRFWGGGGRLLPPGGVADRM
                     LPAIRGEFRVDVREHEDEVMIVADLPGVDKEDVSISLLNPNTLEISCERKMDKEEKEE
                     GYYVRERMYGSMARLVALPHDVTEKGATASFKNGVLEIRMKKSKEARGTRIKIE"
     gene            22526..23230
                     /locus_tag="PKM87_02025"
     CDS             22526..23230
                     /locus_tag="PKM87_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28873.1"
                     /translation="MKAQEKGKGKKKPASRWTAKKIGIIIIGVGFAAIMVVSSLGMGW
                     LVSMNPAAAGDGAVVDVTFFDGMDRPVLTTNPRTFNATFEAGNMVWLCGQFSTRVKST
                     SQTELISIPIYNYYYGEAKYALFSPEWDTISSELEGMKQGESRRITFPALEGFEREMT
                     AEEFNGIGGNFTTVTNGDQVVLAFAENPEISLEENVTPSYVIRTGYVTAKTDEGIRVN
                     YACPSAQISLVQLTKA"
     gene            23275..23541
                     /locus_tag="PKM87_02030"
     CDS             23275..23541
                     /locus_tag="PKM87_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNQ28874.1"
                     /translation="MAIRILYFSQDGCMACSEQEPILLEVERNCGVKVERIAIDPTKG
                     RSLIKEHRLSVTPTILFVREGKELERFEGLVHQEQLSEAIRRHL"
     gene            complement(23536..24117)
                     /locus_tag="PKM87_02035"
     CDS             complement(23536..24117)
                     /locus_tag="PKM87_02035"
                     /inference="COORDINATES: protein motif:HMM:NF039705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HVO_0476 family zinc finger protein"
                     /protein_id="HNQ28875.1"
                     /translation="MERSEEYFCPVCDEETGHEVISRGRNPIARCLSCGSVHPYSPEK
                     EKKLVQVKAIVSAEGTSRVCRVEMEEGEPCRVGDHFVAECGDEYIGVEVMAIERGESR
                     VRKAISSEIATLWTRKVEEVAVRISLHSGRTTASLRTLVPGEEPFVIGEVYKVGQKRI
                     RISRIKMRRGMMLRREGAKAIAREIRRIFAYPL"
     gene            complement(24311..25510)
                     /locus_tag="PKM87_02040"
     CDS             complement(24311..25510)
                     /locus_tag="PKM87_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00297 family protein"
                     /protein_id="HNQ28876.1"
                     /translation="MMNRLGERLAAVLVLSFILIAPFVQPPWFLAIMVVLFSLVLYLI
                     NKTRFLAMALIPIALLYGITILPLLVFSCTLTIMVMGELAFRETEERLYSYIAYIAAA
                     LVGCTLVMAYLGTWAPLVVLFGVVVAVLLKAILKEREDALMIEALGIAMTMFLIYELN
                     YHADMALIGIAVAIAFSFAYFSYRAKTADVSGLFSAALIGIILIIFADIRWFLVVLAF
                     FIMGSASTKYKYDYKLRIGVEQVHGGARGYLNVFANGGVSAAAAILWGVTGSPVFLAL
                     FVGSVATAAGDTMASEIGVTGGTPFLITTFERVPAGTNGGVTLTGEGVALVGAFVISL
                     LAFLLGIVDLPTAAACMLAGFIGTNIDSVVGALMENPGLVGNAGTNFLATLGGGVCAI
                     ALSYPWW"
     gene            25676..28270
                     /locus_tag="PKM87_02045"
     CDS             25676..28270
                     /locus_tag="PKM87_02045"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNQ28877.1"
                     /translation="MSPSLDLPKNYEYSEVERRWQDAWRDEDNYFDPASTKPKFIIDT
                     PPPYPTGTFHIGNAFNWCYIDFIARYRRMRQFNVMFPQGWDCHGLPTEVKVEEIHGIT
                     KNDVPREEFRKMCRELTLSNIEKMRRTLRRMAFSVDWSHEYITMLPAYYGKTQLSFLR
                     MLAAGYIYQSEHPVNFCTRCETAIAFAEVAYEPRETTLNYFDFDGLEIATTRPELLAA
                     CVAVAVHPDDERYRGIPGRRLSVPLFGHEVPLITDPAVDPGFGSGAVMICTFGDKQDV
                     HWWKQHSLELRKAIDRQGRMTEIAGKYAGMKSSECRKAILEDMKGAQILTRQEVLEQR
                     VGTCWRCKTPIEIMSERQWFVKIDQDAIMKAAREITWNPEHMFLRMENWVSQMEWDWC
                     ISRQRLFATPIPVWFCNRCNEMVVPDEKDLPVDPTTSRPSHPCPRCGSTDFVGEEDVL
                     DTWMDSSISVMNVTGWNGASMPPLFPAELRPQGHDIIRTWAFYTILRSVALTGSRPWD
                     QILINGMVLGEDGFKMSKSRGNIISPEEIIEKYGADALRQWAAAGAATGSDIMFNWND
                     VVAASRFQTKFWNIARFVLLQLQRQPYSRDAPIGALADRWLMARLSGTVREVTAAMEA
                     SQFDRALKAIREFSWDVLADNYIELVKGRLYSEGSGRDGACRVLYRTLDTLCRLMAPF
                     TPYFAEECYSYLGKGGVHACTWPEPETEDPEELSNGNLLVQIVAELRRYKHEKGLALN
                     APMGRVAIFSSPITDDCGDAARALNAPLDWHAEPPHLEKVPGDLQFNMAVIGPALRKD
                     AGAFMKAARALPPGLQENPPMELDIGGRKVTVPEGAFSLQYRYLEAGEQVDVISVGDV
                     IVTIHTTP"
     gene            complement(28227..28847)
                     /gene="tmk"
                     /locus_tag="PKM87_02050"
     CDS             complement(28227..28847)
                     /gene="tmk"
                     /locus_tag="PKM87_02050"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNQ28878.1"
                     /translation="MVLITLEGIDGSGKSTLNASLQHALLDLAPVFTREPGATWIGEQ
                     VRRAIADEVDPVTEALLFAADHAAHLDSVIRPALAAGKHVISDRYTDSRYAYQWATLE
                     GVLPDPLTWLRQVHDGWTVPPDHTLLLVLPVEVALSRKKLAGKREHFEEASLLARVQE
                     NYLRLAGEEPSRFVVVDAEMEEGELASFSERLIREWCGSSRSHRRR"
     gene            complement(28838..29509)
                     /locus_tag="PKM87_02055"
     CDS             complement(28838..29509)
                     /locus_tag="PKM87_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNQ28879.1"
                     /translation="MELILAMDLKAGLVVHGASGNRGSYLPLTWGLSSTADPAGYVQQ
                     LAPKHLYVADLDRIMGAGDHTGHVRTCSRYVETCYLDRGCRGPEDYLHDPGIIDIAAT
                     ETSQADLSTYEGGYLSVDVRNGSVMPSGEDPCLLLERAEEWSFEGAIILNVEAVGTGK
                     GVPCQSLAACRSAYDSPLLYGGGVAAPDDLDRLAALGFDGAIVATAVHRGSIPVEDLR
                     RGTWS"
     gene            29554..30240
                     /locus_tag="PKM87_02060"
     CDS             29554..30240
                     /locus_tag="PKM87_02060"
                     /EC_number="4.2.3.153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HNQ28880.1"
                     /translation="MQLLVSPRDIEDARRSLAADIVDVKKPSEGSLGANFPWVVRSIK
                     ALSPKPVSAAIGDFDFRPGGASMAAYGAACAGADYIKVGLMFDGAERARELIQAVVRA
                     VKEEFPEKKVVIAGYSDYQRLGSISPFVISPLAAEAGADVAMVDTGIKDGRSTFEFMS
                     EEALVEFCSVNRESGMATALAGSLRFEDIPALKRIGPDIIGVRGMVCGGDRNDGIREE
                     LVNQLVRMVH"
     gene            30243..31709
                     /gene="guaB"
                     /locus_tag="PKM87_02065"
     CDS             30243..31709
                     /gene="guaB"
                     /locus_tag="PKM87_02065"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNQ28881.1"
                     /translation="MFRDKLEVPLALTFDDVLLIPAPSLVEPNEADIHTRFSRNISLN
                     MPLVGAAMDTVTESAMAMALAREGGIGVIHRNMSPEQEIEEVRRVKRAEELIERNVVS
                     VSPETSVSDVERLMVEHGIGGVPVLEHEKIVGIVSRRDVRAIASRRGSESVKAIMTRQ
                     PITAGEDISMEQALETMYTNKVERLPVVDKAGHLIGIITMQDILEKRQYPNATRDARK
                     KLRVAAAVGPFDIERAHRLDECGVDALVVDCAHGHNLNVVKAIRDIKGSTNADIVAGN
                     IATAAAAEDLIDTVDGLKVGIGPGSICTTRIVAGVGVPQITAIASVADVTRDTGIPVI
                     ADGGIRFSGDVAKAIAAGADCVMIGSLFAGTDESPGRVITMKGRRYKQYRGMGSLGVM
                     SGGVSSDRYFQKKEIGKTKFVPEGVEGVTPYVGTVSDVVYQLVGGLKSAMGYTGSKTI
                     QEMHEKTRFVRITAAGHTESHPHNIMITDEAPNYRISD"
     gene            31814..32431
                     /locus_tag="PKM87_02070"
     CDS             31814..32431
                     /locus_tag="PKM87_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNQ28882.1"
                     /translation="MLDQVEVARLLDILGNRNRRRIIELLRQKPCFVTEISDRLMLSP
                     KAVIEHLQLMEREEILAFRLDERRRKYYFLANDIDVMVRLLRQDQISLVMKDNSHRLR
                     SALARLQKMVETREELAESLKNLERDIDQQIKEIISFGHDVLENETQIDLLISLAHCN
                     LTIQELSELVEAPRDAVSDSLERLRKKHIIEREGETFRLCDAHAQ"
     gene            32421..32858
                     /locus_tag="PKM87_02075"
     CDS             32421..32858
                     /locus_tag="PKM87_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28883.1"
                     /translation="MPSNENPYDEFIRNIARMVEDLLKNMPEENTTRIIGCTIITGNS
                     PNKPHVIFNGDRQDDEITFEVIEGPDRIFVTANLPAFLHSAPYADITPESLHIVVNEK
                     RTKVELPCRVDVIHSYYHVRHGVIDIILKKKAAAPANAGIGQG"
     gene            complement(33073..36678)
                     /gene="nrdD"
                     /locus_tag="PKM87_02080"
     CDS             complement(33073..36678)
                     /gene="nrdD"
                     /locus_tag="PKM87_02080"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HNQ28884.1"
                     /translation="MPTRDTRQSTLDGVFIPSMPPVRTSDGHILEWDRQRIVRQILEE
                     TTLVSTFYGYESASEEMAQEIARLVEERIKSMNLRTLSGPLIREIMNITLLEKGMVQY
                     RNVCTRVGTPVFDAHLIDVGRGFEAKDNANLQENAETSHKKKADKISKEQYLLQLPPH
                     LADHHLSGDIHIHDLEYFGTRPFCIDGATHIPAVIGGRHQVIRPDAFPYDGDVHVPGD
                     LSVLSQKGFRKVRKLTRRPVRGERLLKIRTGSGRALRVTGEHRIPVNEAGMKVVRADT
                     LNVGDRLFSLPENREIRGEPVYSIHLLHELVTRVPPDHLGNVYVRGAGEIFSRLHEEG
                     IKYSEISRELGVSHRKQWYSRGIMPVAVFHRLCERFDITDLSGIRIGAAGSEHDLPLE
                     LPIDPSLVRLLGYFVAEGNYNVSDGKHYNLVITENHQSSAIIADAHASLQCCATRTGG
                     AIASTEIYGVPVTRPRAMQVYMCGKLLWLLFRYVFGVPENSVNKRLPWIVWHLDDELL
                     AEFLSALFTGDGSVYYRPEKSDCIVNYTTSSPALRQELSLLLSSLGMKPHIVELYEEK
                     DRTTIYRIQMNGAVNIRIFSRYARFNDDRQEHINAFLSRVKAISRNTEPEMIVSIEEV
                     DSTGEFVYDIFLEGDGSDESHTYYAADGLLIHNCQDWDLRYFFYYGLMPDGNGTKASV
                     AGPAKRAEVAVLHAVKALGSAQTNFAGGQGYYNFLTFLAPYFEGMDYGEIKQLMQMFV
                     YEMTQMMVARGGQLVFSSVQLSPGVPSLWKDKPVVFQGRVWEGISGPRRTYGEFEREV
                     RLLFKALMEVMLEGDAWGKPFSFPKPEISIEPDFMQEDQAYNEAHPDLPSYQDLYLLT
                     FELASKFGTPYYDNQIPPYRGAGKGISCYQCCAYQFSAVADQDAEFDQKLYFQDGKHF
                     SMGSWQVVSLNCPRAAYRAGGDNERLFSELRALMDVCVEVFRIKRRWLETIRSQGRMP
                     FAMQRPKDPVTGERGSVAVDLDGLVYTIGVVGVNEMVQHHVGSQLHESRDAFRLAILA
                     MTHMEQYARELSKRHNMTIALARTPAETTGQRFAVADLLNKEYRGGAMKVIKGDLEKA
                     LPRLGSTRDLPIYYTNGTHVAPGANVTMAERMSIEHVFFPIVDGGNIFHIWLGESRPN
                     PKGLMEMAMNLCRTTQIGYFAFTRDLTVSLHQFHEYRSDRNRISEWVSAGGRIQA"
     gene            complement(36678..36947)
                     /locus_tag="PKM87_02085"
     CDS             complement(36678..36947)
                     /locus_tag="PKM87_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HNQ28885.1"
                     /translation="MSRQNDLIIYTLEFCPNCDTLKHFLKEKAVPFQERDLSTAESLT
                     ELRINGVFVSEAPVLQRDTRFLTSAEMFSQGAIREEQVIPFIEGE"
     gene            37130..37669
                     /gene="thpR"
                     /locus_tag="PKM87_02090"
     CDS             37130..37669
                     /gene="thpR"
                     /locus_tag="PKM87_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNQ28886.1"
                     /translation="MDRLFVAVELPGEVKEALSSVQDALKASQARLTLVDPDIIHITL
                     KFIGDVPESHTRKIGEVLGMVKADPFPLQVRGIGGNNPRQPRVIWAHIDDGGKCGLLH
                     AQIEDALAPLGVKREARAFRPHATVARVKEFHPDLLERVKPFRERDFGEGVVPGFVLK
                     RSTLTPRGPVYEDVKEVRF"
     gene            37666..39060
                     /gene="cca"
                     /locus_tag="PKM87_02095"
     CDS             37666..39060
                     /gene="cca"
                     /locus_tag="PKM87_02095"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HNQ28887.1"
                     /translation="MTRTPLEEEVLFAIRPAAEETRHIHEVAERILSRVERSGKATAM
                     VVGSVARGTWIHGDRDLDIFLLFDPSLPREELELQGLSLAREVANAESGRFREKYAEH
                     PYINASIDGLDVDLVPCYQVESAARIQSAVDRTPFHTRYIRERIQPFLDDVLLLKQFA
                     KAGGIYGSDQMTEGFAGYLCELLILHYGGFRPLLEAASRWRPGVLIDPEGHRSRDFTE
                     PLVMVDPVDPGRNVSASVSLTRMCEFIELARGYLDHPSREFFFPLHGPQLSIDEAADL
                     LARRETFLYAITLQTPPYIEDIVVPQLRKSLDALQALLDRHGFQVNRADCEMHEEQSM
                     LLFELLVEELPAVKRHAGPPVWNPVNAEKFTAKYLDADERGFFSGPFIDEGMYVVEIR
                     RPYTRAIDLLRSEQVLQVALGKHVKQSMEHSTQVGRGIECYQEEFAQFISGFLQKTSP
                     MMRIERSRRDRTGA"
     gene            39353..40747
                     /locus_tag="PKM87_02100"
     CDS             39353..40747
                     /locus_tag="PKM87_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28888.1"
                     /translation="MPSRRREDALSEVIGFILILAIITILISLYLVYVVPAEGRQNEI
                     GHMNQINYQFSQYKTAIDSLIINEQKDLSIYNTFTLSTQQISSSSGGLFILPLFQPAG
                     SSGAILINQRTDNIEIRGELLQEYAGLGNATPNLSVISGKPSHIYTNFTLRSIYPDKG
                     LNVSWVPPNSGFMVIPLNNPSWKIWINHTPRVDHVSVERVNYETDLTVTVEKNGLVTI
                     QELIIQKNITNNTRYYVDLVDPTYGLGDVLQESEVLQRIPTSAYIEFEPLATNYGYNS
                     TSLVEANTLGSLEFNSENNYFINQDYYYQMGGIFLKQDDGMVNKITPSITLSRDVDNI
                     TYVSIDEIIILGDGIIGGTSPVQVQSFISEIQKPIKTPKGFPNAKWIAVTIETDPESS
                     TMWNETFSRIRLGSSKSPYNVPLEWSSVIKATNSSTFYINGEDDTNTTYDIELNLRRI
                     YLLASLQRIGTILQ"
     gene            40805..>42791
                     /locus_tag="PKM87_02105"
     CDS             40805..>42791
                     /locus_tag="PKM87_02105"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ28889.1"
                     /translation="METDAAVSELVGTILLISLVVIAASIVAVAILSQPIPEDVPQLT
                     AIAENQSQTVYLLHTGGDSLQENEIKTIVNGQDSPFSLDENENWPWSAGKLLKVDYSG
                     PEMPEYVQLIYTGGSTQTLILTTYFVPADVFGAPPTIPPTLPTSIPTTIVTTLPTTIP
                     TPDNGGCSDIIANFNANPITGDIPLLVTFTDTSSSFDPITNRTWNFGDGNVTITSNPT
                     ISHAYTSVGSYTVTLFVANVCGNSNSTTIPNLIVTSCPPVTANFTGSPTSGEIPLTVL
                     FTDTSVSGVPITSRTWNFGDGNITTTNSTTISHTYTSVGSYTVTLTVTNACGNSNSTT
                     IPNFIVTYCPPVTASFTGSPTSGDIPLTVLFTDTSTSGVAITSRTWNFGDGNVTTTSN
                     PTISHIYTTVGSFTVTLTATNGCGQTGTKSTPAFIVTSLACGTITGTKYNDLNANGQR
                     DPGEPGLPGWTIEVYRKQGNNWIFVKNTTTTSDGTYILAGLQYQPAQQYLVKEIIQPG
                     WVRSQPVNADYYNFIVLNPAQCSKTDIDFGNYLPPLRTTLMNTNRGGSIKGGGQVSFT
                     IEEGSSSSNIQIGGMLYNFAVGDAVSLTVNGDSTNVEIDMNGNRISRFSFPDVSVHRN
                     GAFMARGQVTDIWISEYSNIASSLSILVPSMSPVWTRF"
BASE COUNT         9114 a        12673 c        11856 g         9148 t
ORIGIN      
        1 gcggatacgc tccttccagt ccttttgacg ggttctcgtc tggtgggtat accccctcga
       61 gtcccttcga cgatatcccg ttcggcggat acgctccttc cagtcctttt gacgggttct
      121 cgtctggtgg gtatacccct tccaacccct tcatctaatt cttatttcac tcaggttact
      181 gcaaaatttt taccaattca cagggctaaa tccttatatt cttatcccct gtggggtatg
      241 catctgattg catagagaat ctctccctca acccttcctt cccctacaat ctcgcagtcg
      301 aatccctgtt ctgaaaaaat accccttatc ctttcaaggc cagtgagtga agagagcagg
      361 agaagcaccc ttccccctgg tgcaagaacc cttcccacct gcacagcgaa ccgacctatc
      421 acttctcttc cgtcacgacc cccatcgagc gcaagttcaa gccagtcatc aatcctctcg
      481 tccgggaggg tagggaggta aggcgggttg aaaatgacaa ggtcgaaggg cccccggatg
      541 cccgccagga ggtccgcccg tactgcgtcc accttcctcg catatgtctc gcggacggca
      601 tggggattaa tgtccgaagc aagcacccgg ttcttccctg cgagtatccc tgcaatatgc
      661 ccgctcccgc accccacctc gagcacgaag tctccgggct ttgcctcctg aagtgcagcc
      721 tcgagtagga gatacgtgtc ctcctgcggc gggtatacct gggggtgtgt cacgtgcatc
      781 ccaggttggc gatggtggca aagtcttcca ggtagagcgc ctccggcctt gatgagagga
      841 tctcgtctgg cagaccggca agcatgcgtt ccacctgttg cggcgcaatc atcccgcctg
      901 ctcccttcag gcagttacga accgtcttcc tgcggtggga gaagagcgcc ctgacaacat
      961 ctgcgtagag ccggcggtca tggatgggga agaaggggtc gcggggtatg aggtggacca
     1021 ccgttgacct cacgcggggt tggggggaga acgcccgggg ggggaggtca aaaagtcttc
     1081 cagccgcaca gtaggtctgg accataatcg agagtcgtcc gcattcggga gtgccggggt
     1141 gggcaagcat ccgatccgca aactcgctct ggaacatcag gacagcttcg tcaaatcccc
     1201 tctcaagaag gcggaaagtg atcttagagg aggcagaata agggagattc gagacaacca
     1261 cgtcgaactg gggcagcgtg cagcggactg cgtcccccct ggtgagggtg agccggccgg
     1321 cgcggatctc ctcctcgaac tccagggtaa ggcactccac gagttcggga tctatctcaa
     1381 cggcgttgac tcgtgcgcca cgcccgagaa gggcacgggt caggatgccc ctccccgggc
     1441 cgatctcgag cacggtgcgt cctgttactt caatcgcacc ggcaatacgc gcgaccgcat
     1501 ttcggtccac aaggaagtgc tgatcgttcc gtgcgctcat cgggaggtga agaggtgata
     1561 tttgacggtg ggatcctgca gctcctcgag caccctggcg attatcatct tctcggggtg
     1621 aggaatggac ttgatcctct ggcttatctc ggaataactc tcgaacggct tcttctcccg
     1681 ctcctgcagg atctcccaca taagtttctt cccgatgccg ggcaggagat gcagcatgtg
     1741 aagcttgggg ctgatggaga tcgacttgtt gaagaactgc acgaactcgg gctccttctc
     1801 cttcacaatt cgctcgactg cgaaaggaag ctcaagccgt gccgtctggg tcaggtcgtc
     1861 gtagctgatc cggcgcttca cccgctcgat cttctcccgc tccccatcac caatgtacac
     1921 gctctcgtgg atctggatct cgagcgtctt tggtaccatt tcgagaagct tgaactggtc
     1981 cagccccacg ccctggacca gcggctcccg cttgaacaca ggtcgaggat cgtcgggatg
     2041 ccccttctgg aggacatcca gtactatcgc gttgacctcc ttcttctccg tcttcatatg
     2101 atcactcagg agtgcgatct gacgatctcg ataatggtat cgagttcatc ggtctggagg
     2161 gtgaacttct ccttggcgta gatggcgcgg agttcatccc gtgtccgggg catgacattg
     2221 gcgatacgga acgcaatgtc gggcttcatc ttctccatct tcaaaagctc gctcaccagg
     2281 agacgggact tctctgacga tgccttcgag agttgattgg catgctcgat gctcttgcgg
     2341 agctcgtacg acatctcctt ccctgcgtct atccgctctg tctccacctg gtggagcacc
     2401 tcgcggagct cgggaagggt aatcctttct tcacgaataa ttcccttcac cttcatgcca
     2461 atcaccaaaa tgttcgcgtg tgttgagtgt ttacctttgt gcttttagat gttgcggtct
     2521 ggcgatgacg atcttctctt tctccccgtc ccggatcgac aggagccatg cgcggccccg
     2581 ctgcccgacc acagtcccgg tcatcccatg gaaccgcctg tggggcatgc ccttctggat
     2641 gctgggctca cagacgacgt gcaccttctg cccgaggtcg aatttctgga taagcgaggt
     2701 cacagggacc actcctcgct tgcgaaggtc tttcttgaac ttgtaccgtg tcttcttgcg
     2761 tgggccatta tgatgcgcca tgttgcttac tctccctcgt tatgtccttg tacctgcacc
     2821 acgtccaggc tgcacacccg ggcggatctc cccaggaggc cggagaggct cggctcggtt
     2881 ctgccctggt caccggatac cagctccttg atgtagaggc ccgcttcacc agtgacctcg
     2941 aggatcaccc tggcgtctgc ctctcctgcg atgccgatat ccaggacccg gcgttcccga
     3001 acccggtctg cccggcggtg tgcgacccgt agtggggtgc gctggtttat ttttgcccct
     3061 tttagggctc ggattgcgga ttggagctct tcgtaagagt atggtccgtc aatctcgacc
     3121 aggatcctgt attttttata acccttggtc gatttaataa tttccacctc atcgcggctg
     3181 ctccaccgtt cgagggtgac tgagaccctt cccccggcct cccggttgac tgcctcctcg
     3241 atcgtgtgca ggtctgggtt cctcacacgg ggggccacta cctccatcac gaaaggccgg
     3301 ccggtcccga ccatcagcgc gtcgatatcc tccctccccg agccgtggag gatcgcgttt
     3361 tcggccaaaa ataaccgcat aaccggcctg ccgatcagct cctcgactga gtccaggtac
     3421 tgcttgccag tgaaattgca ggcctcgcat ccttgcccct ggcaggcccg gcagtgccag
     3481 tgcgtctggg gaatgccacg ctcgagcttc cgatacctcc catagaagaa tatcggattg
     3541 atctggactt cgacgcactc ctccggaagg ttgaggattg cgaccacctc gggctgcttt
     3601 gggtccgcga tcctgcccgt cttccttgca acagcctttc ccacctcccg gttcatctcg
     3661 gacttcaggg gctctgcatg ggtaagtgag aggtcgctcc atacgatctc ttcgctctct
     3721 gccatcagcg gcgggaccct ggtgccgatc acaaaccgtt caaactcggt ccctccggtt
     3781 gccgcaatca cgcggtctgc ccacacctcc acctgcgaga agaggtcccc gcagatccag
     3841 cacggcccgg gtgactggta tggggtgtta tcctcaaggg acagcgcgat ccggagcgcc
     3901 cgtccccgct cctcgttcgt gaggccaaaa gaccgcttcc cgaagaacct gcccaggcag
     3961 tggtcgcaga tctcaccata cgagaggatc ttctgtgcgt cttcgagtat taccatcccg
     4021 cttccctccg gtcgatctcg ttccagagca ccgtgatggc gtgatcggca tggagcgagc
     4081 gggggcctac tgatatcctt tccagccctt ccaggagttc tctttcaggc gcggtgaaat
     4141 ccaggtggtc gctcagaaga tatgaatcgg gaagtccggc cattcccctg gcatcggtcc
     4201 cgctctcctc gaggactgaa aaggagtact cggccaggag ttcggagagc ccccctcttc
     4261 ggacctcaac gccaggagtc gactcgcgaa acccggtgcc tgcagggact gccagcgcct
     4321 tttttatcag cgatcccgca ctcctctcgt ctgggctcag gtagcggaca ttctctcccc
     4381 tgaaccggac agtcttcggc gcatcgggct cgccgagaag taccaagtaa cactcgacat
     4441 ccctccggaa ctcgtgggag agaaagaatg cggaattgac acacctgcac aggacatcca
     4501 tccggccggc gccaccaggc atatccgaga ggttgaagtc tccggcggtc cgggccaggt
     4561 gccccaccac ggctacccgt cgcatcccgc cccgtcacat cccgccgaac ttcttcatca
     4621 tccgctgcat gttgaacttt ccctgcccgc ccctgacgcc tttcagcgcc ttcttcatca
     4681 tcttgtagta gcggagaagt tccctcacct cttctgggct ggcccctgcg ccggtcgcaa
     4741 tgcgatagat ccgcgaactg cccatcactg aagggtcatc gagctcggtg ctcgtcatgg
     4801 agtccatgat gatgcggtag cgctgcatct tggtgctggt gacgtcgtac gcgtcctggg
     4861 ggatctccat gttcccgagg gggagcatac tcatgatctg cttgagcggt cccatgcggt
     4921 tcaccgcctc cagctgctgg tacatgtccc gcagcgagaa cttgccccgg agcatggcat
     4981 tcacgtcgac ctcctggccg gccatggtct cctcagcccg ctcgacgagt gctttcaagt
     5041 cccccatgcc caggaggcgc gatatgaacc catcgggttc gaagcgttcc aggtcgtcac
     5101 tcgtctcccc gcttccgatg aacacgatcc ctgacctcgt ctcggccacc gcggagagcg
     5161 ccccgccacc cttggcggtc ccgtccatct tggtaatgat gaccccgtcg atattgatcg
     5221 cctcgtggaa ccggcgggcc tgctcggctg cctgctgacc gagggcagcg tcgatcacga
     5281 gccagcggtg ccgtgcggag gcgatcttct ggatgtcgat gatctcctgg atcagctctg
     5341 cctcgagggc atgccgtccc tgtgtatcga tgatcacgac ctcgtttccg gacatcgcgg
     5401 ggaggccttc gcgcactatc ctgatcgcgt ctttttcctt tgggtcccca aaacaggaca
     5461 cgtttatccg ggagcagagg gtattcagct gctcgaaggc acccgggcgg aaggtatcgg
     5521 cgcagatcac cccgacccgg agacccttcc gctggaagta ccgcgcaagt tttgcggtgg
     5581 tggtggtctt tccgctcccc tggagtcctg ccatcaggat gacctggggt tcaagtctcg
     5641 cctcgcctgc agagcctacc agccgtacga gctcctggta cacgatgcgg agcacgtgct
     5701 ccctcaccgt catgcctttc gggggctgct cctcaaggga ccgggtcttg atggcctggg
     5761 agagctgcat caccatcttc acgttcacat cggcctggat gagcgcccgc tgcaggtctt
     5821 ttacgagttc ttcgactgcg gcccggtcga ccacggtctt tcccgcgagc ttcttcagag
     5881 cgtccttgag ggaagtcccc agcctgtcca gcatcaggcc tctaccccca ggagctcgtc
     5941 gaccactcgg gtgggggaga aggggatgat atcatcgtag ccctggccca ccccgaggaa
     6001 cattaccggc tttccaatcg tgtgggcgat tgagatcgcc gcacccccct tcgagtccat
     6061 gtcggccttc gtgagcacga ccgcatccgc cccgactgtc ttctcgaact cgaatgccct
     6121 caccacggca tcgttgccag caacggcctc gtccacgtac acgaccaggt ccggcttcat
     6181 cactctccgg atcttctcca gctggttcat gaggtttgcg cggttgtgga accttcctgc
     6241 agtgtcggca aggacagcat ccacccggtg cgcttctgcg tactggaccg tgtcgtagag
     6301 gactgccgaa gggtcggcgc ccgcctggtg ctggatcacc ttgatcccaa tccttcctgc
     6361 gtgcacgtcg atctgctcga tagccccggc acgaaaagtg tcgcctgcac cgatgaccac
     6421 cgagcagcca ttcttcttca ggtacgatgc cacctttgca accgtggtgg tcttgccggt
     6481 tccgttcacc ccggtgaaga ggatcttcac cggtttttcg tgggtcctga tatacgcaag
     6541 aagatcaaag ccttcaccga gtaccccgag taacgctccc ttcagcgcgg tgacgacaat
     6601 ctcgtcaacg gactctccga tcttcctccg cttcccggtg agatccgccc ttacccgcgt
     6661 gagtatttcg tcggtgacgg ggagagcgac atcgttctca aggagaatca tctccagttc
     6721 gaaaagcggt tcttcgatat ccttctcggc aatgaaaaat tcgcgatcgg tgatgagaac
     6781 tttgaccttc gcaatcaggg aagggtcttt cccggatacc gcaccctcgc attcctcagg
     6841 gacagccgca gtatcggccg gagatgcagt ctttgcgata ttcttctcca gcgttccctg
     6901 gaccttctgg agccttgccc gcagcccctc gaacatcacg cacccccggc aggccgcatt
     6961 atcccaattc ttcgccctgg accggccggg cctgctgcat ctggtactgt tggtacccgg
     7021 cttcaaggcg gcgggcaatc tcattcatct ggtacctgag ctggtcgagg gtctccatga
     7081 ccttcttggc agaggcgtcc atctcggtga ggcgatcctt gaggtattcc accgccgcct
     7141 ccgttgattg ctctaccacg acgtcggccc cgatgttgag gagcactcgt tctggctcct
     7201 caatccgggc ccggacactg acgccccctc cgatctgcag gagtacggta tcttcggacg
     7261 actcctttat gcctgttatc gcatcgatcg cggccagcgc ctccaccctc ccttcttcaa
     7321 gaagggccag ctgctgtgag aagatctcgg cctgctgccg gtattcgttc aggtacagct
     7381 ggagcgactg cagctccctc gggtcaatcc cttccacctt actcaccact gagaagagta
     7441 atggactgca cggtgatata acttcttttc agcctgtgtt tgctcccgat aagggcaaat
     7501 gtccgctctt ttgcctgggc agcgttcggg gcctccacca ctttcgcgta tgggcgccac
     7561 tcgtctccca tcctgaaatc gccttttacc tcatactttt gatccgccat tctctcatca
     7621 ctccaaaaat ccaaatacgt cctctactct tccaagttca aacccggtgg tttcgagccc
     7681 tgtcgcatag ccatgcccgt ttaccaggag gcctgtcccg acaaggccga cgccacagtt
     7741 taccgatccg gtaccgatcg gcactcccgc tgcctgctcc agcacctgga tctcggccct
     7801 cgagctgcga ggatgcacaa gcaccccctt gttggtggcg actccagcca tcccaactgt
     7861 cttgatgccg ccaagggtga gggggaacac ccggactccc aggaactctc ccacttcccc
     7921 cgccatctcc cttggcatat cggggtgcac cgccgcaaag gagtcatttg ccatgatcac
     7981 gttgcctgcg gcgttcatgg tgtgctcgag gaggtatacc tgaccgtact cctcgaggag
     8041 cgcgatctcg cggtcatgcg caaggccgct caccacaaac cccctccggt ttccggcgag
     8101 gagcgacccg actatctcgc tcccctggat agtggtgggg atgatctcca cgtccagtaa
     8161 ctcgattatg gcctcctgga actccgcagg tgcaaccggg gggaccacgg cgaggcctcc
     8221 gattacccgg gtaaaaacgc cgatattcga gtccccggcg atggagatcg tcctgtccat
     8281 ctcactcctc ggcaagttcg gcctggacct gcccgtcatc gaacttcatg gcccggatgc
     8341 gtatgcgcga cggtggtttc tccgccccgc ggtcccagac cctctcgttg atggtgcggt
     8401 ccagtttcac gtcctggctc ttcatatgct ttgccaggaa cttcctgata tcctttatcg
     8461 cggtgttgct cctcttccat cggggagccc tttttacgtc cctcaagggg atgatgtaga
     8521 tctgttcctc catcttgtct gccataaccc ttacaccttc agcgtactcc ttctccagtt
     8581 acgcctgcgg ggatgggtcg agacctggcg tttggtccgc atcatcaccc atgcaggaac
     8641 cctccggttc tgctcacacg cctttgcaag gcgtactttg tatcctttgg ttgatctgct
     8701 catgtgaatt ctcaccctga tattgtaatg gtccccgtga caagggactg ctcgtggtgc
     8761 agggggaaat acttccccga gtcctcgccc cttgcccgca cctcctcgcg aatctccgct
     8821 tcgtagtccc cgttctcgat gcgtcccgtc tccccgaacg caatctccag gtgccgtgca
     8881 gcaatgcggt caacttccct gcgcgggtac ccgagcagcg gtcgcaggta gctgcagccg
     8941 ctcgtgtcct gcagggactg cacttcgtcc cgggaaagca tggggacacg atcatcgaac
     9001 cgggtgccgt cagcgaccac ccggtactcg gtggagagga tgcagagtgc ctccttgtgc
     9061 accatctgga tggcggcatt gggataccct gaggagagga ccatctctac ggcacggccg
     9121 agtaggtcct ccctaaaagt cctccggcga tagggaaacc cgagagcaag tgccgccctt
     9181 tcgacggcag caaggctcct ctgatgactg aacacgaagg tgttcagttc aaccgagtaa
     9241 tccctggaga ggaggactgc cgcaagagag ctgtccttcc cgccgctgaa gaggactccc
     9301 gctttcatcc tttcctggtg atcctgaaat ccttctttgc cggctgcaac tgctgcagga
     9361 gggatttcag ctgcgtatcc gtgatcttct gcttgagacg accgctctgg gcgagcatca
     9421 ccagctgctg ctcaacggcc ctggcaaaat ctggccttgt cagcttgatg gtgttgagcc
     9481 tctctctcgc ttccggctcc aggatctgca tgaggacagc gtggatctgg gcctcaacct
     9541 gcttctggcg ctccatctcc tcctgcatta cctgctggtc gatggattgc tgctgcatct
     9601 gcgccagcct tcttctccgg atttctgcga gttcgtcgtc ccccatggcc ttcaccttac
     9661 ttctggacct gggcgaatcc ttcctttgca atccggtcgg ccaggccgtc catgtaggag
     9721 atgccggaag ggctcaccct gcgaccggtc ttgtcgtgct ggatgaggcc agcgctttcg
     9781 agctgctgga gtgacttcct taagatggac ccgctcccct tcctgaactg ggacgggcgg
     9841 gaacccctgt ccttcttccc tccatagatc gaacgcatcc gctggacgcc gattgggccc
     9901 tctatgtaga ccctccggag gatggatgcc acccggacga accaccactc agggtcctgg
     9961 gggggcatct ccttgtgcac tcctgttttg gcaaatgctg cccatgccgg gggagtgatc
    10021 tccggcttct tcttcagttc ctcggccagg gtgcggatga acctgtcggc cggaacatca
    10081 tagacggttg tcatatgtac tagtacctca ctgtcaccga ttggatataa cagtctttac
    10141 ttatttgttg cctgcacttt tatatatttc gagcatgtgg acgaatccgt gccggcgcat
    10201 tggataatct tcaaattaca gggcttttct ctcggcaagc accgcagtgc gccccctgac
    10261 ctcctgcagt actgccccgg cactccgggc cagttccgcc gggtcgacct ctgtattgcg
    10321 cagccatttc acctttacct ggtggcgttt cttcagctgc tggacgacct cttcgaccac
    10381 ggtcggcgtg agcccctgtt ttccgatcca taccgtggcc ttcatctcct gcatgccgga
    10441 ccgccgccgc tcatccctca tctctgcctc cgcacgggga acctcatatg ctttccgcag
    10501 gagaggcagg tgaccaccac catcccccgc ctgatcctga tcctcacgtt ctcacccggg
    10561 accaggaaat ttccgcaatg atggcagaac tgcctccgga agcacggttc gaggcgtaca
    10621 cggtgtttca tggatatttt ccgggcaagg gcgacatacc tgttgcttga ttccgggtcc
    10681 gcatgaaaac attcgccggc cctggaaaag aggatttcaa tccgttcgcg ggcaatgcgc
    10741 ctcgtgtctg aacgcgggac ctttctcgcc atcacactct ccaaaacagg gaacgggccg
    10801 atgcaccggc agggcatcgc atccatcctc gtcagccctg cgggcctcct tctctcagga
    10861 caacccggcg gccgtcgacc gccagtctgc cctcgcagaa taatttgacc gccatgggaa
    10921 ggcaccggtg ctcttcggca aggatccggc cggcgagact ctcctcgtca tcgtcgtcaa
    10981 ggaccggtac gcatgcctgg acgatgatgg ggcccgtgtc catcccctca tcgacgaggt
    11041 ggactgtgca cccggatacc ttcaccccgt actcaatggc ctgccgctgc gcgtggagac
    11101 cggcaaaact cgggagaagc gccgggtgaa tgttgatcat tttccccgag aactcgcgga
    11161 caatttccgg gcccaggata cgcatgtacc cggccagcac gaagagatcg gaccgaaggc
    11221 ccttcattgc ggtgaggagt gcggcctcgt actcttcctt gcccgggaac cgggaatagt
    11281 ccaccacgtg aacggggatc ccggcatctt ctgccctgct gatggcatgg gcgcggggat
    11341 tgtcggtgac aaggcccacg cacacggcct ggatggcacc gtcccggatc gcatcgatca
    11401 gcgcctggaa attcgacccc ctgcccgagg ccagcaccgc tacccgcttc atctccgggt
    11461 ctcctccgca ctctcctcac ccacatggtg caggatgagc ttgactttca cgatacgccg
    11521 ggagagcatc tgggtgacca cgagggagat atggctctcc tggatgtaga cactctcccc
    11581 cgggtgggga atatggccga ggcgctcgat gataagccct ccgagactct cgtaggcctc
    11641 ggataccgga agagagatgt caagctcgtc gtttaaatcc tccacccata cctttgcatc
    11701 caccaggtag acaccgtcgc tgatcttctg tatctcaggt tcttcgcggt cgaactcatc
    11761 cagtatgtcc cccacaagct cctcgaggat gtcctcgacg gtgacgatcc cgacaaaact
    11821 gctgtactcg tcgagcacga ccgcaatctg gactttccgg agctggagtt ccttcagcag
    11881 gtcgtcgatc ttcttgctct cggggacaaa aaaggggtcg tacatcaggt ctttgattgg
    11941 gatgttcttt ttgccggaga tctggctcga gatgacgtcc ttgacattca gcaccccgat
    12001 gatattgtcc atgtgctcgt ggtagacggg gatccttgaa aacccggtct cgttgaagaa
    12061 atggagtgcg tcctcgagcg tgctctggtc atcgatgagc gccacatcca ccctcggggt
    12121 catgatctcc cgggccgtgg tgtcgccaaa ctcgagcacc gaatagagca tctccctctc
    12181 ttcctgctca attgccccct cttccttccc gacctctatc cattcccgga tctcttcctc
    12241 cgtcacggca ggttcagcga atccgccttc ccccgtcctc cttaccgcaa ccatctcgat
    12301 tgcccagacg aggggggata agatgcggga gaggtagtaa atgggccttg caaccgcgag
    12361 agcaaaccgc tccgtgttcc gcgcagcata tatctttggc ccaatctccc caaataacag
    12421 gaggaggatg accaccaccc cggtggcaat ccctaccccg atgtcgccga atatgctgat
    12481 agtcaccgcg gttgcgatcg ccgcagccgc gacattcacg atgttgttgc caataaggat
    12541 ggtaatcagg aagcggttcg ggtgctcctt gagtgttgca agggcatccg aacctggcct
    12601 gtgctcattg gcaagagtgc ggaccttggc cctggtgatg gagacaagcg ccacctcgga
    12661 tgccgagaaa aatccggaga gtaccaggca gaggatgaac agcaaaagga gtaaaaagtc
    12721 ctcgatcatt cgatccacgc cgcctggacg gcttttttat cgttgttcat atgtcctggg
    12781 acactacatg tatcgtgctg accttttata aaaccgtcga ttatacgaag caatcgcgca
    12841 tgaatgccaa ttccctggag aggacgagga tcttctcctc ggatgagagg tcatgggaga
    12901 agttccggtc ttctatctca agggtcaggt tcccgtcgta gccggacgcc gacaattgac
    12961 cgaggatgtc tgcgatctcc tcgtcaccat cgattgggag gtgcatcttt ccggactccg
    13021 ccctccccac atgcacattc acaatcctgt ctttgcacag ttcgatgtag cgttgggcct
    13081 cgcctgggcc tgctcccatg gcatgagaga tatcaagtgt gaacccaagc catggttccc
    13141 gatccagcag ctccttcatc ccttcaggag tgcagaggag cgagtttatt tttcgctcca
    13201 tgttctccat ggccactgta acgccaaaac ccgttgctgc ttcttcgagt gcgcggatat
    13261 acctggcgaa gcgttcataa tcgggagcgc tgggaggcct ctttgcggtc cgccggcccg
    13321 ggtggaccgt gacgacaccg ctgccgaccc gtgccgcaag gcagaccgcc tccaccgtgt
    13381 accgcacgga aacctccgcg actgcggggt tgattgaggt cgggttcagg tcgagaacag
    13441 gtgcgtgaat ggtaggagag accagctggg aaaacgctcc caggtttttc ataagttcct
    13501 cttcgggttg tcccgccatc cagtaacccg gggtctccac ccagaattcc accgaatcga
    13561 agcctgcctc cacgatgtat gagaatatct cttccagggg atattcatgg aaaaacatgc
    13621 ttgatacggc gaaatgcacc atgatcctaa ttcattaccc gccggccata atagttgttg
    13681 atgacctggt accagaaggg gatctccgat gagcggggcg ggcacaccac agaggtgctc
    13741 tccgtctcac aggtctcctc gctcgtggag gcgctcctgg atgacacgag gcttcaggac
    13801 atctgggtcc gtggagagat cacgaacctc aagcttcacg gctcgggcca ttgttacttc
    13861 tcgctcgggg agaagagcag ggaccattca gcggtcctct cctgcgtgat gtggcgtaat
    13921 gatgcaagaa gggtggagtg cgagtttcgc gacgggatgg acgtggttgt gtatggctcg
    13981 atcgggcatt atgctcccca gggccgttac cagctctatg tgagggaggt caggccggcc
    14041 ggccggggcg agaagcacct gatggtggaa aagtggaagg tccagctgac ctccgagggg
    14101 tgctttgatc cgcagaagaa acggccacta cccgcgtatc cgctcaaggt gggagtggtc
    14161 acctccgaga ccggcgcagt cctccaggac atccggaacg tggtatcccg tcgctatccc
    14221 cttgaactcg tggtctcccc cactgcagtg cagggagagg gggcgcacct tgagattgca
    14281 cgtgccctgc aaagggtgga gcgggacgtg gatgtgatca tccttgcaag gggcggggga
    14341 agtttcgagg acctgttccc cttcaactac ccagaggtgg taagggcgat agtctcgtgt
    14401 cccgtgccgg tggtgagcgc gatcgggcac gaggtggaca taaccctcgc ggactttgcc
    14461 gccgatgtca gggccccgac cccttcagcg gcagccgaac tggtggtccc cgacaggata
    14521 gcactcatgg attctcttgc tctgttcagg aaaaccctgg gaaacaggct cattgtgcgg
    14581 ctggaccgtg cagggaccga tctctccgag gtgcgtgaga ggctctcccc ccggagggtc
    14641 tcgcagaggg tgaccgcgaa aagagaagac gtctcggtcc tttcaggacg gatggaaagg
    14701 gcggtcctgt cgaggatcgc gctggagagg gaacggctgt ctgccgctgt gggggtgctt
    14761 gccgcaagaa atcccgccca gctcctccgg aggggctact gcatcctgca gaaggatggg
    14821 cgggtaatcc attcggcaga cgagatcgcc tgtggagacc gcctcaccct ccgtctcgtc
    14881 gatggccggg cggtcacgcg cgtggaagag gtgcatcatg actcataaat ttgaagacct
    14941 gatgaaggaa ttgaaagaga tcctggcaaa gatcgaggac aaccagaccg ggctcgacga
    15001 agcgatcgcc ctttatgaac ggggcgcggt ccttgtccgg caggcagagg aactcctggc
    15061 atccgcggag atgaagatca cgatgctcgg gagggactaa cctccctgtg actctcaggg
    15121 gaccctgacc gtgagggtgg ccacaacctc gcctcccttt cggagagcct ctacgaactc
    15181 ccggggcatc atgcgggcgg tagtgtcaga accaatcgcc acggtcctgc cgcacacgta
    15241 gcagctcctt ctccatacca tgtcggccgg gtggtcaagt gtcattgaag aggacccctg
    15301 cccccggacc tccacgctca tccccccgca tgagaagact gtatgaagag aggcgtcatc
    15361 cctgcagagg aggatgcgga actcctccgg gaggtcgcgt gcactgcaat cagcaccaac
    15421 cgcgataata cagtccccat tgggggtcat ttcgtgctcg caggttatct cgagcgtgga
    15481 gggatgagcc gccctcacat gcggatgacc cctgcaatgg ataatggccc tggcttccct
    15541 tttcattcct gaataatggt atatgaaaga agagtaaaga gatgatgcat tgggagtgtc
    15601 aggctcccgt gacggcacaa cccctaaaaa ggggcattcc tttttttttg actcacgcat
    15661 gccccatatc tccctttttt cgaacttttt tttagtattt gtaccagcgc cctttctcgt
    15721 cgaattcacc ctgttggact ggggaggcgt ccgcgggaat ctcatctgaa gtatcgcgga
    15781 agaagactgc cttgaaagta cagatgatat ttcccgcgac catcattgca acaaggtaga
    15841 atatcgcggt aatgaggatc ccttcctgcc caagcatctc gttgaacatg gagacagaga
    15901 atgcctggac ctgggagggg tccatggtag cgagagggag gagctgttcg tagagaagac
    15961 ccgtccagat caccgccagg ggtattgcca ccagcaccac gatgccaagg cacaccaggt
    16021 aaaatacaat caccctccct ggccggttca tcaccacctc cacgctccgg cggatacagt
    16081 caaagaccct gcggtcctcg aagactgccg cggtatcaaa gaagaatgtg aagaagatga
    16141 tgggaattgt acatcccatg accacaaacg ggagggcggt cccggccagg cccatgaacg
    16201 taaggggtat tacgaccaga aagactgttg caagcacggc gaacacgatc acgagcgagg
    16261 ggaggaggat ccggaaataa taggctgtcg ccccggcaac aaaggctact gcgcccttct
    16321 cgcccgagcg tatctggacg tatgcgccgg caaggaggaa cgggatgagg agggcctcga
    16381 taaaccagag ccgtgttgca aggaatgcac ccagataata ctgcagcagg agatccgccg
    16441 caccgagcgc acccgccgcg aggccaggga gccagaggaa tgggtttttc aacagcaggc
    16501 cacacgcacg cttcagcgag tcgaggacca tccgttcacc tgttccttgg catcgccacg
    16561 atctcgcgaa cctgcaggtc aaagaacgag gcggtgtgtg acgggcggac cacgcagacc
    16621 gtatctgctc cgcttgccgt tcccccgacc gcgattacct ccttgtcgac aggaatgacc
    16681 ccctggtcgg cggcaatcag cgtgcattcg actgcaacct tcagaccgac cgagatcaca
    16741 cgccggagcg cctccgcgac ggcctccgtc cgggagcctc cgcccacctt cggggagcgg
    16801 gagaacgcac gctccatccc ggaaaggaca tgcgtcccgg tcacaagggg gatcttctca
    16861 agcttcaggg tccgggcagc ctcgggcgag aactcccatt cacccggccg cgagaacccc
    16921 acgacgtgcg tgacgaccac cagcttgatg cctgagcccc ggagcatccg gtaaaacgca
    16981 agggccgtgt ctcccgaagt gctggccacc acaaccctct caattgacag ttcacgcgcc
    17041 ctttcaaggg caaacctggc acagtccacg gtgttggcag ggccgggcgc atcaaaataa
    17101 taacagttcc tctcgacaaa cgacataggc accctaaggt taaggtggta ccaggtaaag
    17161 ggaattgcta tgatcacgct cgcactcgcc gggaagccaa actgcggcaa gtcgaccttt
    17221 ttccgggcgg cgacgatggc acacgccgag attgcaaatt acccattcac caccatcgac
    17281 gcgaacttcg gagtggccta cgtccgcacc gaatgccctt gcaaaagcct cgggctccag
    17341 tgcggcaact gtgccgatgg catcaggttc gtgccggtga acctgatcga cgtggctggc
    17401 cttgtcccgg atgcccacaa gggaaagggc ctcgggaacc agttcctgga ccacctgcgc
    17461 caggcggacg caatcatcca catcctcgac gcgagcgggt ccacggacac cgaggggaat
    17521 cccgtcgaga caggcagcca cgacccgcga gaagatatcg cattcctcca gaaggagatg
    17581 acaatgtggg tatacgggat cctggagaag cactgggcaa ggctccagcg ccaggcccag
    17641 tcaaaggcgt attcgatcca caggggcatc tccgaggtct tcacgggcct cggcatcacg
    17701 ccggaagatg tccaggacgc ggagaggagt gttgcgatcg acctggcccg ggcagaaccc
    17761 gaaggccttg taaagttctg tgccgagatt gttgcgatca gcaagcccat gatcccagtc
    17821 gggaaccggg ttgacatggc tcctgctccc ctgctggaca cgctccgctc cgcggggatc
    17881 acatttgcca ccgcggcagg ggagctcgcg ctccggaatg ccgtgtcggg cggcctgatc
    17941 tcctatcacc ccggcgaccc cgcgttctca ataatcaaag aggaatccct ctcacctgcc
    18001 cagaaagcag gtctccagac aatcgctgca gtcatgaagg ccaacggggg gacgggggtt
    18061 cagcaggcca tcgacagggc agtctatgaa ctgctcggga tgatcgttgt ctaccccgtc
    18121 gaggacgagc acaagttctc ggactcgcgc ggccggattc tccctgacgc gttcctgatg
    18181 aagagaggct cgaccccgag ggaccttgca ttccaggtcc ataccgatat cggaaaggcg
    18241 ttcctctacg ctgtcgacgc aaggacgaag atgagggtga aggacaccca cgaactcaaa
    18301 cccggcgata tcattcgcat cgtgagtgcc gcgaaatgac cgcttttttg gggaatacgg
    18361 ggagaaaacc gttatttctg agatagtttt atatagtggc aattgccaac cggagtatta
    18421 gcatgggtgg cgacgtattc caggcccaga tattgaggaa tttcttcgat accatcacgg
    18481 gtaccgaccg gaacctcacc cgcatttaca tgtgcgtggt gagccttgcc aagatccgga
    18541 tggagccccc agaaaaattt actttcctgc tcgaacagct gcggaagagc aagcagcaca
    18601 aggagctctc ggtggatatc cttgactaca tgtgtgacgt ggctgcccag ctcgagatcg
    18661 cggcggtcca gaccgcgttc ggggtaaaag aggtcgggga gatccagcag gagttcggaa
    18721 gcatcagcat ggactctctc tgatccttcc ataccatact gttttcccgc agccgcagtt
    18781 gcaatcccat cccgctggaa cgagagagag aaaaatggcg tgagttattt gaggttcagt
    18841 ggctcgcggt aactctcgag gatctggagg acgaagttct ggtgttcgtc caaagactgc
    18901 ccgtcgtccg gggactggag atatgcaaca gtatagagaa gcaggagggc attgtcaagc
    18961 gtcagggtat cctggttctc gatctgctcc tctttgagga caatgggcag ccaggaagca
    19021 tcccccgcct cgaaccttac cggcgagtaa tcgccaaccc tggcctcttc gagcgcaagg
    19081 gcacgatcca tgagaatccg gagtgccttt ccaatcacct cgccggcccc gtcttttggg
    19141 attcgggaag aagcttttcc ggcatcggtt actgtctttg catacagccc aaacttgaag
    19201 gagaggacag ctgatgcaat ctcgtgcgca gtctccgggt caatgccccg gaagagaggg
    19261 gtcatgcgat gtatataatt gtacaggtat ttttccatgg agtcttcctc tttggcaatg
    19321 gtttttggag tgctgaagcg gtaatgaagc ctgatcacca ggagcgtgat actcacggca
    19381 tacagtatct ggaggaggaa ggacgccttc gtctcaaggg gaattacaaa ggcgaggagt
    19441 gcatatgccg gtgcgaagag agagacgagg tctttgcggg gctttatgta tgcgagatac
    19501 gccaggatca gcgacgcggc aacgcacccc cagtaaaagt cggccgcatc cgcgatgagg
    19561 cccgcctcct tgagaaggat tgcgagtatg agacctccga atgcacagag cggcacggca
    19621 agggcgagct ggatctcacg cttcttcctt acatggtcag ggttctgtga tgaattggct
    19681 gacaagatac cattcagatg tggggacccc accagaaaaa gatttcaata tgcccccggg
    19741 agtctgctca tttgtcctgg cagaccaaag aacgccattc tcccagatgg tttaaacgta
    19801 tgcaggtgga accaatggat atgaaggtgt gcatcatgtg cgggggagag gggacacgcc
    19861 tccgccccct cacgttcgag cgccccaagc cctgcattcc catcgtgaac cgcccctcga
    19921 tacagcacct tgtctctcac ctctccaacc tcgggttccg cgagatcgtg gtcaccctcg
    19981 ggtacatggg ggatgcaatc gaggatgcgc tgggcgacgg gtcgctcttt ggggccgata
    20041 tcacctacgt gcacgagaag atcaagctcg ggactgcagg gagcgtgaag aatgcccagg
    20101 cataccttga agaaggagat ttcctcgtcg tgggtggaga ccatgtcacc gatctcaacc
    20161 tccttgattt ctaccgggag cattcccggc accagccgcc ggtctccatc gggctgatca
    20221 gcatcgatga acccggagaa tacgggattg cggagatcga tgcggacttc cagatcaaga
    20281 ggttcaagga gaaaccggcg ccgggagaga tcttctcgaa tctcgccagc accgggatat
    20341 atgtctgcag gccggagatc ttcgatgaga tccctctggg aaggaagttt gactttgcca
    20401 gggatctctt cccgcacctc atggagtccg gccatgtgct ccgggcctgg ctcgcacggg
    20461 ggaactggac cgacgtgggg aaccctgcat cactccgcca ggctgaacgc tggaaactgc
    20521 aggagatccc gtatacgagt atcagcggcg atctcacgat cagggccggg cagatccagg
    20581 ggccggtcca gctcgggacc tcattctcga tgggtgtcaa ttcgcgaatc atcggcccgg
    20641 tggccatcgg gcccgggacc gccattggag atcgtgtcct catcggccct tacacgagta
    20701 tcggtgagaa ttgcctggtg aagagcaacg cgaagatatt ctcctcctcc ctttacaacc
    20761 gggtggtggt agggaggaac agcaccgtgt caggaagcat catcgacaat gacacggtga
    20821 ttggggacga ctgcagcctc gagaacgaca cggtcatcgg gccgagggta gtgttcagga
    20881 acggggtggt cgtccattca aggacgagga tctggcccga ggtggtggtg ccggacggaa
    20941 cagtcgtaaa agaacacctc ctgaatgacg aatacgcggt gaaatgcgag gggtcctagc
    21001 ccttccggac caggcggtgc gtgatggcga ccagaggatg gcgggcgtag tcgaggacct
    21061 ggatatcatc aagcgatcgc aggttcatgg cccgggcggt cctctccatg ccgagttcga
    21121 tatcctcgtt cacctcgatg acataggcat cgagtgtctt aattcccatc ttccgggcag
    21181 caatcgccct gtggtgcccg tcgacaagga tgagcttatt cggcctcctc acgacaatga
    21241 ggggttcggc gagtcctttt tttatctcgt agatgcgccc ctcgagttcg tcttcgtaaa
    21301 tgcgtgactg ggtgggaagg agctggtcta tcgggacgac ttcccgcttg agctcagggg
    21361 agatcccatg gagcgtcttt aacgtcgtga tgaagttgaa gaccttctct ggggagacat
    21421 gctctatctg ggatcggatc acgtcagtat tggatatgat tccaagcagc ctgttctgct
    21481 cgtctaccac ggggagtttc tggatgccgg aacggaagat gaccctcgct gcatcgttga
    21541 tgctcatgtc agggtcagcc acgatcaggt gctcggacat caccttccgg acaggggtcg
    21601 taactggctg aaagaggagg tccctggcgg ccacgtaacc caccacctcg tttcccatca
    21661 cgacagggaa cccgtcatgg ccggtcttct ggatcttctc catgacctcc cgcacggtac
    21721 cctgggcatc cacggtgacg acgtcgtagg tcatgtagtc cctgaccttc ttcttatcca
    21781 tcgtacctca tttcagggtc aaaagactaa aaaccatcgg aaaaaaaggg agattattcg
    21841 atttttatgc gggtcccccg cgcttccttc gatttcttca ttcggatctc gagcaccccg
    21901 ttcttgaagc tcgcggtggc ccccttctcc gtgacatcgt ggggaagggc gacaagcctt
    21961 gccatggagc cgtacatcct ctccctgacg taatacccct cctccttctc ctccttgtcc
    22021 atcttccgct cgcacgatat ctcgagggtg tttggattca ggagggagat cgagacatcc
    22081 tccttgtcga cgcctgggag atccgccacg atcatgacct cgtcctcatg ctccctgaca
    22141 tccacccgga actctcccct gatcgccggg agcatccggt ctgccactcc gccaggcggg
    22201 aggaggcgcc cccctccacc ccagaagcga ttctccatct ctcccatcag gtcctcgaag
    22261 tcgcgcatca tccagccaaa cggatatctc cttctgtata ccatctgaaa cctcctttcc
    22321 ttctccaccg gcaaaggcca atggactaac tttatgtgac tgtttataat atataaacca
    22381 tttgtatgag gaaataccca gttatccaca gaccatgtca gacctggatc gtttttcaaa
    22441 ggacccgata ggtttgcatg gtagtagagg ctctctccac atacacgctg taacgtttat
    22501 tttcccagga accaatgata aaaggatgaa ggctcaagag aaaggaaagg ggaagaagaa
    22561 acccgcttca cgctggactg ccaagaagat cggcattatt atcatcggag tcggttttgc
    22621 ggcaatcatg gtagtcagct ctctcgggat ggggtggctt gtgtccatga accctgctgc
    22681 tgcgggcgac ggggcggtgg tggatgtcac cttttttgac gggatggatc gccccgtgct
    22741 gacaaccaat ccgaggactt tcaatgcgac attcgaagca gggaacatgg tatggctctg
    22801 cggccagttc tcgacgcggg tgaaaagcac cagccagacg gagctcatct ccatccccat
    22861 atacaactac tattacggcg aggcgaaata tgccctcttc tctcctgaat gggacactat
    22921 ctcaagcgaa ctggaaggaa tgaaacaggg tgaatcccgc aggatcacat tccccgcgct
    22981 cgagggattc gagcgcgaaa tgactgcaga ggagttcaat gggatcgggg gtaactttac
    23041 caccgttaca aacggtgacc aggtagtcct tgcttttgcc gagaaccccg agatctcgct
    23101 tgaagagaat gtcaccccca gttatgtaat ccgcaccggg tacgtcactg caaagaccga
    23161 cgaaggcatc cgggtcaact atgcctgccc atcggcccag atctcccttg tacaactcac
    23221 caaagcctga aaaattccat agctttttta taatcccccc tcgttttttt ctgcatggca
    23281 atccggatac tctatttctc ccaggatggg tgcatggcct gcagcgaaca ggaaccgata
    23341 ctacttgagg tggagaggaa ctgcggggtg aaggtggaga ggatcgcaat cgaccctacg
    23401 aagggacgct cactcatcaa agagcaccgc ctgagtgtca ccccaaccat actctttgtc
    23461 cgtgagggaa aagaactgga acgtttcgag gggctggttc accaggagca gctcagcgag
    23521 gcaatccgcc ggcacctata gcggatacgc aaaaatcctc cggatctcgc gggcaatggc
    23581 ctttgcaccc tccctgcgga gcatcatccc cctccgcatc ttgatcctgg agatcctgat
    23641 cctcttctgg ccaaccttgt agacttcccc tatcacgaac ggctcttccc ctggcaccag
    23701 agtccgaaga gacgcggtgg tccggcccga atgaagagag atcctcaccg ccacttcctc
    23761 aacctttctc gtccagagcg tggcaatctc gctgctgatc gcttttctta ccctcgattc
    23821 accgcgctcg attgccatca cctcaacacc gatatactcg tcaccgcact ctgccacaaa
    23881 atggtctccc acacggcagg gctcgccctc ctccatctcg accctgcaga ccctcgatgt
    23941 cccttcggcg ctgacgatgg ccttcacctg gacaagcttc ttctcttttt ccggtgaata
    24001 ggggtgaacg ctcccgcacg agaggcacct ggcaatcggg ttccttcccc gcgagatcac
    24061 ctcgtgaccg gtctcttcat cgcacacagg gcagaagtat tcttcgctcc tctccatttc
    24121 cccatcacca ttcctggtat accattccat ctttcatgaa atatacactc tggcccctct
    24181 acctcaaatt cgaaacaatg gcacctggcg ccaccctctc tcccgcacat cccggtgaaa
    24241 tgactgccct ggcatgatag atagtggggg tttttgcaac cagggggcct ttcagaaaga
    24301 ggttcctccc ttaccaccac ggatatgaga gggctattgc acagacccct ccgccaagcg
    24361 tcgcaaggaa gttcgtgccg gcatttccga caagccccgg gttctccatc aatgccccaa
    24421 ccacgctgtc gatattggtc ccgatgaacc ctgcaagcat gcaggccgca gcggtcggga
    24481 gatcgacgat gccaaggaga aacgcaagca gtgagatgac aaaggcgccg acaagcgcaa
    24541 cgccctcccc tgtaagagtc actcccccgt tcgtgcccgc ggggacacgc tcgaacgtgg
    24601 tgatgaggaa tggtgtgccc cccgtcaccc cgatttcgct tgccatggtg tcgcctgcgg
    24661 ctgttgccac gcttcccacg aagagggcga ggaatacggg ggaccctgtc actccccaga
    24721 gaattgcagc ggcggcagag acacccccgt tcgcaaagac attcaggtac cccctggcac
    24781 ccccatgaac ctgctcgacg ccaatccgga gtttatagtc gtacttgtat tttgtactgg
    24841 cagagcccat gatgaagaac gccaggacca ccagaaacca gcggatatcc gcaaagatga
    24901 tgaggatgat tccaatcagc gctgcagaga aaagaccgct cacgtcggct gtctttgcac
    24961 ggtacgagaa gtatgcaaaa gagaaggcga ttgcaacggc gataccgatc agcgccatat
    25021 cggcatgata attcagttca tatatgagga acatggtcat ggcgatacca agggcctcga
    25081 tcatcagggc gtcctcccgc tccttgagga ttgccttgag gagaacggcg accacgacgc
    25141 caaagagcac cacgagcggc gcccaggtcc caaggtaggc catcacgagc gtgcagccta
    25201 ccagtgccgc ggcaatatag gcgatgtaag aatagagccg ctcctctgtc tccctgaacg
    25261 caagctctcc catcaccata atcgtgagag tgcatgaaaa gacgagaagc gggaggatgg
    25321 tgataccata aaggagggca atagggatga gggccatcgc gagaaaccgc gtcttattga
    25381 taagatagag aaccagcgag aagagcacca ccatgatcgc gaggaaccac gggggctgga
    25441 caaagggtgc gataaggatg aaagagagca cgagaacggc agcgagtcgc tcccccagtc
    25501 ggttcatcat tctttttttg ttggtccgga caagataaga gcttttgtct gcgcaagaca
    25561 ggaacgcaat acagagccgt ttcccctgaa tcagtcttat tccggagatg ccggccagtc
    25621 tgccagtatt tgcattcgat tagatatatc tcctgcaaag acccatattg agaaaatgtc
    25681 gccgtctttg gatctcccca agaattacga atattcggag gtggaacggc gctggcagga
    25741 cgcctggcgc gatgaagaca actattttga cccggcatcc acgaagccca agttcatcat
    25801 cgacacccca cccccctatc ccaccggcac gtttcacatc ggaaatgcct tcaactggtg
    25861 ctatattgat ttcattgcca gataccggag gatgaggcag tttaacgtga tgtttcccca
    25921 gggatgggac tgccatggcc tgccgaccga ggtgaaagtg gaggagatcc acggcatcac
    25981 caagaacgat gtcccaaggg aagagttcag gaagatgtgc agggaactga ccctctccaa
    26041 catcgaaaag atgcgccgta cactgcgccg catggccttc tccgttgact ggagccacga
    26101 gtatatcacg atgctccctg cctactatgg aaagacgcag ctctctttcc tgcgaatgct
    26161 tgccgcaggg tatatctacc agagcgagca ccccgtcaac ttctgcacgc ggtgcgagac
    26221 cgccatcgcg tttgctgagg ttgcatacga accccgggag actaccctca attacttcga
    26281 cttcgacggc ctggagatcg cgacaacgag accagaactg ctcgcggcat gcgtggcggt
    26341 tgcagtccac ccggacgacg aacgttaccg tggcattccg ggacgaaggc tctccgttcc
    26401 tctcttcggc cacgaggtcc ccctgatcac cgaccccgcc gtcgacccgg ggttcggatc
    26461 cggggccgtg atgatctgca cgttcggaga caagcaggat gtccactggt ggaagcagca
    26521 cagcctcgaa ctccggaaag ccatagaccg gcagggccgg atgacggaaa tcgcaggaaa
    26581 atatgcaggc atgaaatcct ccgagtgccg caaggcaatc ctcgaggaca tgaaaggagc
    26641 acagatcctc acccgccagg aagtcctgga gcagagggtc ggcacctgct ggaggtgcaa
    26701 gactccgatc gagatcatga gcgagcgcca gtggttcgtg aagatcgacc aggacgcgat
    26761 catgaaggct gcccgcgaga tcacatggaa cccggagcac atgttccttc ggatggagaa
    26821 ctgggtctcg cagatggaat gggactggtg catctcccgg cagagactct tcgcaacgcc
    26881 gattccggtc tggttctgca accgctgcaa cgagatggtg gtccccgatg aaaaagacct
    26941 ccctgtagat cccaccacgt cccggccttc ccatccctgc cctcgctgcg gctcgacaga
    27001 ttttgtcggg gaagaggacg tcctcgatac ctggatggac tcttccatct cggtcatgaa
    27061 cgtaaccggg tggaacgggg cgtccatgcc gccgctcttc cctgcagagc tccgcccgca
    27121 ggggcacgat atcatcagga cgtgggcgtt ctacaccatc ctccgttccg tggcccttac
    27181 cgggtcgcgg ccatgggacc agatcctcat caacgggatg gtgctcggcg aggacggctt
    27241 caagatgagc aagagccgcg gcaacatcat ctcccccgag gagatcatcg agaagtacgg
    27301 ggccgatgct ctccgccagt gggcggccgc cggtgcagcc accggctcgg acatcatgtt
    27361 caactggaac gacgtcgttg ccgcctcccg cttccagacc aagttctgga atatcgcccg
    27421 attcgtgctc ctccaactcc agcgccagcc ctattcccgc gacgcgccca tcggggctct
    27481 tgccgaccgc tggctcatgg cccgcctctc cggcacggtg cgggaggtga ccgctgcgat
    27541 ggaggcaagc cagtttgacc gcgccctgaa ggccatccgc gagttctcgt gggatgtgct
    27601 tgccgacaac tacatcgagc ttgtgaaggg gaggctctat tccgagggaa gcggccggga
    27661 cggtgcctgc agggtgcttt accgcaccct cgacaccctc tgccgcctga tggcgccatt
    27721 caccccttac tttgcggaag agtgctactc ttacctcggc aaggggggcg tgcatgcctg
    27781 cacctggccc gagcccgaaa cggaggaccc ggaggagctt tcgaacggca atctcctcgt
    27841 gcagatcgtt gccgaactgc ggcgatacaa gcatgagaag ggccttgccc tgaatgcccc
    27901 gatgggcagg gttgccatat tctcgtctcc catcacggat gactgcgggg acgcggcgag
    27961 ggcgctcaat gctccactcg actggcacgc cgagccgcca caccttgaaa aagttccggg
    28021 cgatctgcag ttcaacatgg cagtcatcgg tcctgcgctc cggaaagatg cgggagcatt
    28081 catgaaggca gcacgggcgc tgcccccggg cctccaggag aacccgccca tggaactcga
    28141 tatcgggggg cggaaagtta cggttcccga gggagcattc tcacttcagt acagatacct
    28201 cgaggcggga gagcaggtgg acgtgatcag cgtcggcgat gtgatcgtga cgatccacac
    28261 cactccctga tcaaccgttc agaaaaagag gcgagttctc cctcctccat ctccgcgtcc
    28321 accaccacga accttgaagg ctcttctcct gccagtcgga ggtaattttc ctgtacccgt
    28381 gcgagcaggg acgcttcttc aaagtgctcc ctctttcccg ccagtttttt ccgcgatagc
    28441 gccacctcaa ccgggaggac gaggaggagc gtatggtcag gggggaccgt ccacccgtca
    28501 tgcacctgcc ggagccaggt gagggggtcg gggagcaccc cttcgagagt agcccactgg
    28561 taggcatacc tgctgtcggt gtaccggtcg gagatgacat gcttcccggc tgcaagtgca
    28621 ggcctgatca cagaatcgag gtgagcagcg tgatcagcag caaagaggag tgcctcggtc
    28681 accgggtcca cttcatctgc gatggccctc cggacctgct cgccgatcca ggtggcgccc
    28741 ggctccctcg tgaataccgg tgcaaggtcc aggagagcat gctggagcga ggcattgagg
    28801 gtgctcttgc cactgccgtc tattccttcc agggtgatca ggaccatgta cccctccgca
    28861 ggtcctcgac cgggatcgaa ccgcggtgca ccgcggtcgc gacgattgcc ccgtcgaagc
    28921 caagcgccgc cagcctgtcc aggtcatcgg gagccgccac gcctccgcca taaaggaggg
    28981 gagaatcgta cgcactcctg caggcggcca gcgattggca gggcactcct ttccctgtcc
    29041 ccaccgcttc cacgttcagg atgattgcac cctcgaaact ccattcctcc gccctttcga
    29101 gaagtaagca gggatcctcg cctgatggca tgacgctccc gtttcgcacg tccacgctca
    29161 gatagcctcc ctcgtaggtt gaaaggtcgg cctgagaggt ctcggtcgct gcaatgtcga
    29221 tgatcccggg atcatgcagg taatcttccg gtcccctgca accgcggtct aggtagcagg
    29281 tctctacgta acgactgcag gtcctgacgt ggcctgtgtg atcgccggcc cccatgatcc
    29341 ggtccaggtc ggcaacgtac aggtgtttcg gggcgagttg ctgcacgtat cccgcaggat
    29401 ctgctgttga tgagagcccc caggtgagcg ggaggtagga accacggttc cccgatgccc
    29461 cgtgcactac caagccggcc ttcaaatcca tggccaaaat caattccatg ctattagcgc
    29521 aatcactatt agccagcagg atcattaaat cctatgcaat tacttgtcag cccgagggac
    29581 attgaggatg ccagacgttc tcttgctgct gatatcgtgg acgtgaagaa gccgtccgag
    29641 ggatctctgg gtgcaaactt tccatgggtg gtacggagca tcaaggccct ctccccgaag
    29701 cctgtcagcg ccgcaatcgg tgatttcgat ttccggccag ggggcgcttc catggccgca
    29761 tacggagctg catgcgcagg ggctgactat atcaaggtag ggctgatgtt cgacggggcc
    29821 gaacgtgcga gggagctgat ccaggccgtc gtgcgggccg tcaaggagga gttccctgag
    29881 aagaaggtgg tgatcgcggg ctactcggat taccagcgcc tggggagcat ctcccccttt
    29941 gtgatatccc cccttgctgc ggaggccggg gccgatgttg caatggtcga caccgggata
    30001 aaggacggac gcagcacgtt tgaattcatg agcgaggagg ctctcgtgga gttctgctcc
    30061 gtcaaccggg agagcggcat ggcaaccgca cttgcagggt ccctccgctt cgaggatatt
    30121 cccgccctga agcgaatcgg ccccgacatt atcggggtca ggggaatggt gtgcggtgga
    30181 gaccggaatg acggaatacg cgaagaactg gtcaaccagc tggtcaggat ggtgcactga
    30241 ccatgttcag ggataagctc gaagtcccgc tggccctgac ctttgatgac gtcctcctta
    30301 tccctgcacc gtcgctcgtc gagccaaacg aagccgacat tcatacccgt ttctcacgta
    30361 acatctccct gaacatgccg ctcgtgggag cggcaatgga caccgtgacg gaatcggcaa
    30421 tggccatggc gctggcccgc gaggggggca tcggggtcat tcaccggaac atgtcacccg
    30481 agcaggagat cgaggaggta cgccgggtca agcgcgctga ggagctgatc gagcggaacg
    30541 tcgtctcagt cagccctgaa acgagcgtct cggacgtcga gcgcctgatg gtggagcacg
    30601 ggatcggggg agttccggtc ctcgaacacg agaagatcgt ggggatagta agccgaagag
    30661 acgtccgtgc aatcgcttca cgccgcggct ccgagagcgt gaaggcaatc atgacgaggc
    30721 agccgatcac tgccggtgaa gacatctcca tggagcaggc gctcgagacc atgtatacca
    30781 acaaggtgga acggctcccg gtggtggaca aggcgggcca cctgattggg atcatcacca
    30841 tgcaggacat cctcgagaaa cgccagtacc cgaatgccac acgcgacgca aggaagaaac
    30901 tcagggttgc cgctgcggtc ggaccgtttg acatcgagcg ggcgcaccgc ctcgacgaat
    30961 gcggcgtaga tgcactggtg gtggactgcg cccacggcca taacctcaac gtggtaaagg
    31021 caatcaggga catcaagggg agcacaaatg cggatatcgt tgccggcaac atcgccaccg
    31081 ccgccgcggc cgaagacctg atcgataccg tcgacgggct gaaagtcggt atcgggccgg
    31141 ggtcgatctg cacgacccgc atcgtggccg gggtcggcgt cccccagatc accgccatcg
    31201 cgagcgttgc agacgtgaca agggataccg gaatcccggt catcgccgat ggggggatac
    31261 ggttcagcgg agacgtggcc aaggccatcg cggcaggagc cgactgcgtg atgatcggaa
    31321 gcctctttgc agggaccgac gagtctccgg gaagggtaat cacgatgaaa ggacgccgtt
    31381 acaagcagta taggggaatg gggtctctcg gcgtgatgag cggcggagtc tcaagcgacc
    31441 gatatttcca gaagaaggag atcgggaaga caaagtttgt ccctgaggga gtggagggtg
    31501 tgacccccta cgttggcact gtctccgatg tcgtatacca gcttgtcggc gggctcaagt
    31561 ctgcgatggg gtacacggga tcaaagacca tccaggagat gcacgagaag acaaggttcg
    31621 tgcggatcac cgctgccggg cataccgaga gccaccccca caacatcatg atcactgacg
    31681 aggctccgaa ttaccggatc tcggattgaa gacccaattc tttttcgatc ggaaaaatct
    31741 ctcttttata tcggcttccc tggtgaattt ttcccaaggt ataaatactt ccataactaa
    31801 catgaagtaa tcaatgctgg accaggtaga ggttgcccga ctccttgaca tccttggaaa
    31861 ccggaacagg cgccgtatca tcgagctttt gagacagaag ccctgtttcg tcaccgagat
    31921 ctccgaccgc ctgatgctct ccccgaaagc cgtgatagag cacctccagc tgatggagcg
    31981 cgaggagatc ctcgcgtttc gcctcgatga gcgcaggagg aaatactact tcctcgcaaa
    32041 cgatatcgac gtgatggtca ggcttctcag gcaggaccag atcagcctcg tcatgaagga
    32101 caactcccac cgccttcgct ccgcgcttgc ccggctccag aagatggtgg agaccaggga
    32161 ggaactggca gagagcctga agaaccttga gagggacatc gatcagcaga tcaaggagat
    32221 catctcgttt ggacacgatg tcctggaaaa cgagacccag atagacctcc tcatcagcct
    32281 ggcccactgc aatctcacca tccaggaact ctcggaactc gtggaggccc cgagggatgc
    32341 cgtatccgat agccttgaaa ggctccggaa gaaacacatt attgagcggg aaggggagac
    32401 cttccgcctc tgtgatgcac atgcccagta acgagaaccc ctacgacgag ttcatcagga
    32461 acatcgcgag gatggtggaa gacctcctga agaacatgcc ggaggagaac accacccgca
    32521 tcatcggctg cacgatcatc accgggaaca gtccgaacaa gccccacgtc atcttcaacg
    32581 gcgaccggca ggacgacgag atcacatttg aagtgatcga ggggccggac aggatctttg
    32641 tcaccgctaa cctgcccgcc ttcctgcact cggcccccta cgcagacatc acgcctgaat
    32701 ccctgcatat cgtagtgaac gagaagcgga cgaaggtgga actcccctgc agggttgacg
    32761 tgatccacag ttactaccat gtccgccacg gggtcattga cattatcctg aagaaaaaag
    32821 ccgccgcccc cgcaaatgcg gggatcggtc agggatagtg ccgctaccct attattccgt
    32881 ccctttttta ctcaattgca tgaggagcct gaccccgggt atcctcgctc taaaaggtac
    32941 ttgacagttg agaatggtgt ggagcgtcgg agtacagccg cgcaggttcc acccggcgaa
    33001 aacaggtgag ggaccggata tggtcccccg ttgctccttt ccacagaaga aatgacgatt
    33061 gagaaaaatg gttcaggcct ggatgcgtcc gccggcagac acccactctg atatccggtt
    33121 cctgtcgctc ctgtattcgt ggaactggtg aagagagaca gtaaggtcgc gggtaaatgc
    33181 aaaatacccg atctgtgtgg tcctgcagag gttcatcgcc atctccatca ggccctttgg
    33241 attcggcctc gactcgccaa gccagatatg gaagatgttg ccgccgtcca caatagggaa
    33301 gaagacatgc tcgatggaca tccgctccgc catggtgaca tttgcaccgg gggccacgtg
    33361 ggtcccgttc gtgtagtata tgggcaggtc ccttgtagaa ccgagacgtg gcagggcttt
    33421 ctcgaggtcc cccttgatca ccttcatcgc accgcctcgg tactccttgt tgaggagatc
    33481 cgcgaccgcg aaccgctgcc cggtcgtctc ggcaggggtg cgggcgagtg cgatggtcat
    33541 gttgtgccgc ttcgagagct cgcgggcata ctgctccatg tgggtcattg caaggattgc
    33601 cagcctgaag gcatccctcg attcatgaag ctgcgacccg acgtgatgct gtaccatctc
    33661 gtttactccc accaccccga tcgtgtagac caggccgtcc aggtcgactg caaccgatcc
    33721 ccgctccccc gtgacaggat cctttgggcg ctgcatcgca aacggcatcc tcccctggga
    33781 ccggatcgtc tcgagccacc tccgcttgat gcggaagacc tcgacacaca cgtccatcag
    33841 tgcccggagc tctgaaaaga gccgctcgtt gtcccctccc gcacggtagg cggccctcgg
    33901 gcagttgagg gagaccacct gccaggagcc catcgagaaa tgcttgccat cctggaaata
    33961 cagtttctga tcgaactcgg cgtcctggtc ggcgacagca gagaactggt aggcacagca
    34021 ctggtagcat gagatcccct tgccggcccc cctgtagggg gggatctggt tgtcatagta
    34081 gggggtgccg aacttcgatg ccagttcgaa cgtgaggagg tagaggtcct ggtagctggg
    34141 aaggtcgggg tgcgcctcgt tgtatgcctg gtcctcctgc atgaagtcag gctcgatgct
    34201 gatctctggt tttgggaaac tgaacggctt gccccaggcg tccccctcga gcatgacctc
    34261 catcagtgcc ttgaagagga ggcggacctc ccgttcgaac tccccgtatg tcctgcgggg
    34321 gccgctgata ccctcccaga cccttccctg gaagacgacc ggcttgtcct tccagagaga
    34381 gggcacgccc gggctgagct ggaccgagga gaacacgagc tgcccgcctc ttgcgaccat
    34441 catctgggtc atctcgtaga caaacatctg catcagctgc ttgatctccc cgtaatccat
    34501 cccttcaaag tagggggcga ggaatgtgag gaaattgtaa tatccctgtc cgccggcaaa
    34561 gttggtctgg gcgctcccga gcgctttgac tgcgtggagc accgccacct ccgcacgctt
    34621 cgcaggcccg gcaaccgatg ccttggtccc gttcccgtcg ggcatcaggc cgtagtagaa
    34681 gaagtagcgg aggtcccagt cctggcagtt gtggatcagg agaccatcgg cggcataata
    34741 ggtatggctc tcatcggatc cgtctccttc aaggaaaatg tcgtatacaa attccccggt
    34801 agagtcaacc tcttcgattg agacgatcat ttcaggttcc gtattgcggg agatggcttt
    34861 tacgcgggag aggaacgcat tgatatgctc ctggcggtcg tcgttgaaac gggcatatct
    34921 tgagaagata cggatattta ccgccccgtt catctggatg cgatatatgg tggtccggtc
    34981 cttttcttcg tatagttcaa cgatatgcgg cttcatcccc agtgacgata ggaggaggga
    35041 aagttcctgg cggagggcgg gcgaagaggt ggtataattg acgatgcagt cggatttctc
    35101 cgggcggtaa tagactgatc cgtctcccgt gaagagcgcg gagaggaact cggccaggag
    35161 ctcgtcatca aggtgccaga cgatccaggg gaggcgctta ttcacagagt tttccgggac
    35221 gccaaacacg taacggaaga ggagccagag gagcttgcca cacatgtaca cctgcatggc
    35281 ccttggccgc gtgaccggaa cgccgtaaat ctcggttgag gcgattgcac cccctgtcct
    35341 cgtggcgcag cactgcaggg aagcatgggc gtcagcgatg attgccgacg actggtggtt
    35401 ctcggtgatg acaagattgt aatgctttcc atcactcaca ttgtagttcc cctctgcaac
    35461 gaaataacca aggagccgga caagggaggg atcgatcggc aactccaggg gaaggtcatg
    35521 ttcgcttccg gcagcaccga tcctgatgcc ggagaggtca gtaatgtcga acctctcgca
    35581 gaggcgatgg aagactgcaa caggcattat cccccggctg taccactgct tccggtggga
    35641 caccccgagt tcccgggaga tctcgctata ctttatcccc tcttcgtgca ggcgggaaaa
    35701 gatctcccct gctcccctga catacacatt cccaaggtga tcaggaggta cccgcgtgac
    35761 gagttcgtgg aggaggtgaa ttgagtagac cggttccccc ctgatctcgc gattctctgg
    35821 gagggaaaag agcctgtctc caacgttcag ggtatctgca cggacgacct tcatccccgc
    35881 ttcattgaca gggatgcggt gttcccccgt tacccgaaga gcccttcccg acccggtcct
    35941 gatcttcagc agccgctctc cccttacagg ccgcctggtg agtttgcgta ctttcctgaa
    36001 tcctttctga gagaggacag agagatcgcc aggcacgtgg acatctccat cgtaggggaa
    36061 cgcatcaggc ctgattacct gatgccggcc cccgatgaca gcggggatat gcgtcgcgcc
    36121 atcgatgcaa aacggccttg taccaaagta ttccaggtcg tggatgtgga tatcgcccga
    36181 gaggtggtga tccgcgaggt gcgggggcag ctgcaggagg tactgctcct tgctgatctt
    36241 gtctgccttc ttcttgtgag atgtctcggc attctcctgg aggttggcat tgtccttggc
    36301 ctcgaaaccg cgcccgacat cgatcaggtg cgcgtcaaag accggtgtcc ccaccctcgt
    36361 gcagacgttc ctgtactgca ccattccctt ctcaaggagg gtgatgttca tgatctcccg
    36421 gatgaggggg ccgctcagcg tgcgaaggtt catcgacttg atacgctcct cgacgagccg
    36481 cgcaatctcc tgggccatct cctccgatgc gctctcgtag ccgtaaaagg tggagacaag
    36541 cgtggtctct tcgaggatct ggcgcacgat ccgctgcctg tcccactcca ggatatgccc
    36601 gtcagaagtc ctgacaggcg gcatgctggg gatgaagacc ccgtcaagag tggactggcg
    36661 cgtatcgcgg gtgggcatca ttcaccctcg atgaagggga tgacctgctc ctctcttatt
    36721 gccccctggg agaacatctc ggccgacgtg agaaaccggg tatccctctg gaggaccggg
    36781 gcttcggaga caaagacacc atttatgcga agttcggtga gcgattctgc ggtagaaaga
    36841 tcccgctcct ggaatggcac tgccttctct ttcaggaaat gcttcagggt atcgcaattc
    36901 ggacagaatt ccagtgtata tataatcagg tcgttttgtc tgctcaaagg tgtccccccg
    36961 gtttggatct gtttcaatga tagctcttct gtgaaaaaat atttgttgat ctgacagtaa
    37021 ttatacctta agaattgaga atttttgcaa aaaaaagcac atttaacgcg tgatagatga
    37081 tgaaccgcac aatggtctgc gacgggatgc ctgcgcggga gcccatctaa tggacaggct
    37141 ctttgtggcc gtggaactcc cgggagaggt gaaagaggcg ctttcttccg tgcaggacgc
    37201 ccttaaggcg agccaggcac ggcttaccct tgtggacccg gacatcatcc acatcactct
    37261 caagttcatc ggggacgtgc ccgagtccca cacaaggaaa attggagagg tgctcggcat
    37321 ggtgaaggct gacccgttcc cgcttcaggt gcgtggcatc ggtgggaaca acccgcgtca
    37381 gcccagggtg atttgggcac acatcgacga cggggggaag tgcggcctcc ttcatgcgca
    37441 gatcgaggat gcgctcgcac ccctcggggt aaaaagggag gcccgcgcgt tccgtcccca
    37501 tgccacggtg gcacgtgtaa aagagttcca ccccgatctc ctggaacgcg tgaagccatt
    37561 cagggagagg gactttggcg agggagtggt gccggggttt gtcctgaagc gaagtaccct
    37621 gaccccccgg ggtcccgtat acgaggacgt gaaggaagtg cgcttttgac ccggacccct
    37681 cttgaagagg aagtactctt tgcaatccgg cccgccgccg aagagacccg acatatccac
    37741 gaagtggcgg agaggatcct ctcccgcgtg gagaggtccg gcaaggcaac cgccatggtg
    37801 gtgggatcag ttgcgcgggg cacctggatc cacggcgacc gggacctcga catctttctc
    37861 ctctttgacc cctcgcttcc aagggaggaa ctcgagttgc aggggctgtc ccttgcccgg
    37921 gaggtggcaa acgccgagtc cgggagattc cgggagaagt acgccgagca cccatacatc
    37981 aatgcgagca tcgacggcct ggacgtggac ctggtgccct gctaccaggt ggagtccgcg
    38041 gcccggatcc agagcgcggt cgaccggacg ccgtttcaca caaggtacat acgggagagg
    38101 atccagccgt tccttgacga cgtcctcctc ctcaagcagt ttgcaaaggc tgggggaatc
    38161 tatggatccg accagatgac ggaagggttt gcagggtacc tctgcgaact cctcatcctc
    38221 cactacggcg ggttccgccc gcttctcgag gcggcgtcac gctggaggcc cggcgtcctc
    38281 atcgacccgg aaggacaccg ttcgcgtgac tttaccgagc cgctcgtgat ggtcgacccg
    38341 gtggatcccg ggaggaacgt ctcggcatca gtctccctca cccggatgtg cgagttcatc
    38401 gagctcgccc gcgggtacct ggaccaccct tcaagggagt tctttttccc gctgcacggg
    38461 ccgcagctct caattgacga ggcggcagac ctcctggcac ggagggagac cttcctctat
    38521 gcaatcaccc tccagacccc gccctacatc gaggacatcg tcgttccgca actgcgaaag
    38581 agcctcgatg cattgcaggc cctccttgac cggcacgggt tccaggtgaa ccgtgccgac
    38641 tgcgagatgc acgaggagca gtctatgctc ttgttcgagc tcctggtgga ggagcttccc
    38701 gcagtgaaac ggcatgccgg cccgccggtc tggaacccgg tcaatgccga gaagttcacc
    38761 gccaagtacc tcgatgcaga cgagcggggg ttcttctccg ggcccttcat cgatgaaggg
    38821 atgtacgtcg tcgagatccg ccggccgtac acccgtgcaa tcgacctttt gcggtcagag
    38881 caggtgctgc aggtggcgct cggaaagcat gtcaagcagt cgatggaaca ctcaacacag
    38941 gtggggcggg gcatcgagtg ctaccaggaa gagttcgctc aattcatatc ggggttcctc
    39001 cagaagacgt ctccgatgat gcggatcgaa cggagcagga gagatcggac cggggcctga
    39061 aggggcgatt ccgggacttt tttcgaatga aacgcacatg ataccgatcg gtcagatgcc
    39121 cccctggtga aaaccgcgga tgcggttttc ataccaaaat gcctgcagtt tttttatgcg
    39181 tgtgtcagga agagaatcat gtagtttttt ccctgtgttt tccagttacc cgaattctgc
    39241 agatgccttc cattctggta aaaaggactc tttttgcgca aaaatccccg tttaccgata
    39301 gatcttttga tacttttata taatgacccg ccacacatac tcaataatct ccatgccgtc
    39361 cagacgccgc gaggatgccc tctccgaggt tatcgggttc atcctgatcc ttgctattat
    39421 taccatcctc atatccctct atcttgtgta tgtggtaccg gcagaaggac gacaaaatga
    39481 aattggccac atgaaccaga tcaactacca attcagccaa tataaaacgg ctattgattc
    39541 attgataatt aatgagcaga aagatttgag tatctacaat acgttcacgt tgagtaccca
    39601 acaaatcagt tcgtcatcgg gaggtttgtt tatactcccc ttgttccagc ccgcaggatc
    39661 atccggggca attctcatca accagaggac agataacatc gaaattcggg gagaactact
    39721 tcaggaatac gccggattgg gaaatgcaac tccgaatttg tccgttatat ccggtaaacc
    39781 atcgcatatc tacaccaatt tcaccctgcg ttccatctac ccggataaag gattgaacgt
    39841 cagctgggtt cccccaaatt caggtttcat ggtcattcct ttgaacaatc cgtcatggaa
    39901 aatctggatc aaccacactc ccagagttga ccatgtgagt gtcgagaggg taaattatga
    39961 gaccgatctg actgtcacgg ttgaaaagaa tggccttgtt actatccagg aacttattat
    40021 ccagaaaaat atcacgaata atacccggta ttacgttgac cttgtagatc ccacatatgg
    40081 tttgggtgac gtactccagg aatctgaagt gcttcagcgt atccctacca gcgcctacat
    40141 tgaatttgaa ccgctggcaa cgaattatgg atataacagc acatcccttg ttgaggcaaa
    40201 cacgttaggt tcgctagaat tcaattcaga gaacaattac ttcattaacc aggattatta
    40261 ctaccagatg ggaggtatat tcctgaaaca ggatgatggg atggtgaata aaatcacccc
    40321 gtcgatcaca ctctcccggg atgttgacaa catcacctat gtttccattg acgaaataat
    40381 aattctggga gatggaatta tcggggggac ttcacctgtc caggtacaat cgttcatatc
    40441 ggaaatacag aagccaataa aaacccccaa aggatttccg aatgcgaaat ggatagcagt
    40501 tacgattgag acagatcccg aatcatcaac gatgtggaat gaaacgttct ccaggatccg
    40561 gcttggttcc agtaaatctc cttataatgt cccattggaa tggagctctg taataaaagc
    40621 aaccaattca tctacttttt atataaacgg agaagatgac acaaatacga cctatgacat
    40681 tgaactgaat ttaaggcgga tatatctcct tgcctctctc cagagaattg ggactattct
    40741 ccaataatac cccccccaca atggaagctt ttatctgttc tatccttaaa ggatgaagtg
    40801 aacaatggaa accgatgctg ccgtatctga acttgttggt acaatacttc tcatttctct
    40861 tgttgttata gctgcctcga ttgttgcagt cgcgatacta tcgcaaccta ttccagagga
    40921 tgttccgcaa cttactgcta tcgctgaaaa ccagagtcag acggtatatc tccttcacac
    40981 cgggggtgat tccctccagg aaaacgagat taagactatt gtaaatggac aggattctcc
    41041 attttccctg gacgagaacg aaaattggcc gtggtccgca ggaaagctgt tgaaggtgga
    41101 ttattccgga ccagaaatgc cagaatatgt ccaattgatt tacactggtg gatcgaccca
    41161 aacgttaatt ttaacgactt atttcgtccc tgctgacgtg ttcggtgcac ctccgaccat
    41221 ccccccgacg ctacccacat ccattcccac aaccattgtc acgacattac caacaacgat
    41281 tcctacacct gataatggag gatgctctga tattatcgcg aattttaatg caaaccctat
    41341 tacaggcgat attcctcttc ttgtcacatt tacagataca tccagctcat ttgatccaat
    41401 aacaaacaga acttggaact tcggcgatgg taatgtgaca attacctcaa accccaccat
    41461 ctcgcatgca tacacttcag ttggaagtta tactgtcaca ctgttcgtag cgaacgtttg
    41521 tgggaatagt aattcaacca caattccgaa tcttattgtg acttcctgtc ccccggtcac
    41581 cgcgaacttc accgggagcc caacttctgg agagatacct cttaccgtcc tatttaccga
    41641 tacgtcagtt tcaggagtac ctattacaag cagaacatgg aacttcggcg atggaaacat
    41701 taccaccaca aattccacca ccatctcgca tacttacact tcagttggaa gttataccgt
    41761 tacattgacc gtgacgaatg catgtgggaa tagtaattca acaacaattc cgaatttcat
    41821 tgtaacttac tgcccgccgg tcaccgcgag cttcaccggg agcccaactt ccggcgatat
    41881 tcctcttacc gtgctgttta ccgacacatc aacatctggc gtcgcaatta cctccaggac
    41941 ttggaatttc ggcgatggca atgttacgac cacctccaat ccgacaatat cccatattta
    42001 tacaacagtc ggctctttta ctgtcactct tactgcaacg aacggatgtg ggcagactgg
    42061 aacgaaatca actccagcgt ttattgtgac ttccttggct tgtggtacga ttacggggac
    42121 aaaatacaat gacctaaacg cgaatggcca gcgcgatcca ggagagccgg gactacccgg
    42181 ctggaccatc gaagtctata gaaaacaggg gaataactgg atctttgtaa aaaacacaac
    42241 aacaacatca gatgggacat atatacttgc aggcctgcaa taccaaccgg cacaacagta
    42301 cctggtgaaa gagattatcc agccaggatg ggtaaggtct caaccggtaa atgcggatta
    42361 ttataatttc atcgtactca atccagcaca atgttccaag actgacattg actttggtaa
    42421 ctaccttcca ccattaagaa ccacactcat gaatactaac cgcggaggat caataaaagg
    42481 tggcggccag gtatcgttta ctattgaaga gggaagcagc agttcaaaca tacaaattgg
    42541 tggcatgctg tataattttg cagtcggcga cgcagtctca cttactgtca acggggacag
    42601 cacaaacgtc gagattgata tgaatgggaa ccgtatctcg aggttctcat tcccggatgt
    42661 ttctgtccat cgcaacggag cttttatggc acgtggacag gttacagata tttggatcag
    42721 cgaatacagc aacattgcct catctctttc cattctcgtc ccatccatgt ctcctgtatg
    42781 gacacggttc g
//
LOCUS       DAOQYR010000009        42025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_1041_length_42025_cov_8.559018, whole genome shotgun sequence.
ACCESSION   DAOQYR010000009 DAOQYR010000000
VERSION     DAOQYR010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 42025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42025
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1041_length_42025_cov_8.559018"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..933
                     /gene="rfbA"
                     /locus_tag="PKM87_02110"
     CDS             52..933
                     /gene="rfbA"
                     /locus_tag="PKM87_02110"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNQ28890.1"
                     /translation="MKGIILAGGFGTRLYPVTKSVSKHLLPVYDKPLIYYPLSVLMLS
                     GIREILIISTARDVPLYRELLGDGRQVGLSFSYAVQEKPGGLAEAFIVGEKFIGTDAV
                     ALVLGDNIFYGQHFSELLRKAASQKHGATIFGYWVRDPRPYGVVEVGPDGQALSIEEK
                     PAKPRSHYAVPGLYFYDNRVIGIAKSISPSARGELEITDLNREYMKKGELFVLVFGRG
                     MAWLDAGTHDSLLEAGNYIETIQKRQGLYVACIEEIAYRQGFIGKDQLRALADGLKNT
                     EYGRYLAELAENESEMQ"
     gene            1077..1370
                     /locus_tag="PKM87_02115"
     CDS             1077..1370
                     /locus_tag="PKM87_02115"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNQ28891.1"
                     /translation="MKSRLEILSILNNLKQELSPRYHIRRLGIFGSVAKDAHTPTSDV
                     DILIEFDYPISGFSFVHLKELLSSSLEMKVDLVTPGGLHPLLADSIQSDVLYV"
     gene            1363..1734
                     /locus_tag="PKM87_02120"
     CDS             1363..1734
                     /locus_tag="PKM87_02120"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNQ28892.1"
                     /translation="MSEKRAYALFLYDMACAVDKILEYTKSLSRQNLLENELVKDAIL
                     RNIQVLGDAAKKVPPEFIASHQEIDWKGIAGLRDIITHQYFRVDWNILWTTIQEDLPP
                     LSSTLHSLLERSLADDNGRSV"
     gene            1731..2321
                     /gene="rfbC"
                     /locus_tag="PKM87_02125"
     CDS             1731..2321
                     /gene="rfbC"
                     /locus_tag="PKM87_02125"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011450012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNQ28893.1"
                     /translation="MTSPKFSPIPTSLSGAVLIEPRVFGDDRGFFLESYTRRDMAALG
                     IPEEFVQDNHSRSAKGVLRGLHFQCPFAQSKLVRVARGAIYDVIVDIRRGSPTFGRHE
                     SFLLSEENHRLLYVPVGFAHGFLALAEGTEILYKAGEYYHPEADAGIRWNDPDLAIPW
                     PVSEHGIAAPLLSDKDARLPLFSDIESPFLYQGASR"
     gene            2494..3345
                     /gene="rfbD"
                     /locus_tag="PKM87_02130"
     CDS             2494..3345
                     /gene="rfbD"
                     /locus_tag="PKM87_02130"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011450011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNQ28894.1"
                     /translation="MKVMITGAGGQLGQEMTKQAATRGYDTIPFTSRELDITVAGAVR
                     GAILRHRPDAVINCAAYNAVDQAERDWKRAFLVNGLAVRNLALAAHEAGAVLVHYSTD
                     YVFDGLSPRPYTIADRPNPLSRYGESKLLGEQAVRDHSRRYFLIRTSWVFGPGNTNFA
                     QKVIEWSRQNDRLKIVDDQVSSPTCTRDLADATFGLLETGQFGLYHLTNGGSCSRYEW
                     AKYILDATGWKGVLLPAKSEEFPTPALRPAYSVLDNFGAAEVRGQELPPWEDATRAYL
                     EEIGGIP"
     gene            3342..4406
                     /gene="rfbB"
                     /locus_tag="PKM87_02135"
     CDS             3342..4406
                     /gene="rfbB"
                     /locus_tag="PKM87_02135"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNQ28895.1"
                     /translation="MTHILVTGVAGFIGSNFVYHVLGTHPEISIVGLDSLTYAGNLEN
                     IAALGRKERERFTFVRGDIGDRPLADSLISEHAIDAVVNFAAESHVDRSIEDPQLFLR
                     TNILGTCTLLDAARAAWQRNGRMTPGCRFLQVSTDEVYGSLGPEGYFTEETPLDPHSP
                     YSASKAAADMIAKAYHDTFGMPVLITRCSNNYGPYQFPEKLIPLIIRNALQHRPLPVY
                     GDGKNVRDWLYVEDHCRAIDVVLRSGQPGEVYNIGGNNEWENIAIVRLLLSILQEETG
                     DPGINESLIQFVKDRPGHDQRYAINSTKISRELGWEPATPFPDGIKKTVRWYLDHQDW
                     MERVISGEYQSFYERNYEHR"
     gene            4560..5642
                     /gene="wecB"
                     /locus_tag="PKM87_02140"
     CDS             4560..5642
                     /gene="wecB"
                     /locus_tag="PKM87_02140"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNQ28896.1"
                     /translation="MICLVLGTRPEVIKLSPLIRECGRLEIPYYLLHTGQHYSYEMDR
                     VFFEDLELPAPQFNLDTGSGLHGEQTGKILAGAERVFMEHRPGAVLVQGDTNTVLAGA
                     LAAAKLHVPVGHVEAGLRSFDRRMPEEINRIVADHVSDLLFAPTETAAGYLRSEGIPG
                     EKIFVTGNTVVDAVFQNLEIARKKTGVMERMGAKEGDFILVTAHRAENVDDRGRLAGI
                     LEGIGAVGEETGMQVIFPVHPRTAKMIGEFGLDTPGIRIVPPLGYLEFLLLESKAALI
                     LTDSGGIQEEACVLGVPCVTLRENTERPETLQVGSNVLAGTNPTTIRESAREMRSRDR
                     GWENPYGDGHAGERIIGILATMGLTG"
     gene            5639..6964
                     /locus_tag="PKM87_02145"
     CDS             5639..6964
                     /locus_tag="PKM87_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28897.1"
                     /translation="MTTGRCRDQKDSYPHLGAVAPWTGMRAGLVTGSRVRVRDTPRNN
                     VTRAFSPSLPFQQRVIPMRSVRFFVLFILVCLALPAAAVTITERPDTIANGQPIFVTI
                     RDLPGNSSFSLLVQADCAVEPNTEFRFQMSQFVMPFALKKSSITATLSGTSTNRLEVM
                     KESTIVTVSGQSRDGQFSTTKHYDITPGTYDYFRLSGTSLPTARSITAQLQVTGTTQG
                     PKDSEISFVVEGLTAGTITIAALVDGTQVLYKSIPIGVSESSSAGDGVPVATTAPPPE
                     QWNTFTSADGLVSVTVSHAGPIGFLKVPPVDVTWGMQAISGPYTLVPQDTSFLPPGRI
                     EFRIPAGAPRDGLVVASLDRETWTILSSTIGNTSVTAPVTISGTYALLGPVPVLATTT
                     TTAITPIATTVEETPPTPTPADTTKAGIGGATLILAICAGCAVAMRKIQ"
     gene            complement(7026..>7769)
                     /locus_tag="PKM87_02150"
     CDS             complement(7026..>7769)
                     /locus_tag="PKM87_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ28898.1"
                     /translation="CHYAVDPAGWFVAEADGEIVGTVAVTNYDSRFSFGGFFVIREDM
                     RSKGVGWQLSSAAIRHAGDRNLGIDGVYEMQEKYSSRMGFRFAYRNIRWRGIADGRRQ
                     AGLGSAQEVPFSALVAYDALHFRAERKRFLERWIRTPGSWSSALVEGGSLRGYGVIRR
                     CFEGYKIGPLFADTPEIGECILEDLTGRDEVRGQLFFFDTPEPNRDAVAMAQEREMEE
                     VFGTARMYTKEIPPLPVGRIFGVTTFEMG"
     assembly_gap    7770..7869
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8164..9825)
                     /locus_tag="PKM87_02155"
     CDS             complement(8164..9825)
                     /locus_tag="PKM87_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNQ28899.1"
                     /translation="MKVRILLILLVLGAALLASGCITPEKEKTEASVASATLNLAATA
                     IDAELADVRALNLESAGRLSRTGLAGPDAEAVLFEKLDALPWALSSVTISPEGILVAA
                     VPEHYRGIVGRDVNYDGPVQEALAMKVPRVSEVFMMEEGFAGISQSAPVFAADGTYLG
                     YTDVTYRPETLIGRAIAPVVEGTAYDIWVTQTDGTVIYDTTQEEIGKNLFSDPVYQEP
                     GLQEFFRRVVSEPAGTGSYRFWDREWDKEIEKEAAWETAGIDGAAWRVVLTEGKIQSG
                     TADASFVRNASLAGLSDLDADVQSAAAFAAMEGKEAALREFNDPAGRFVDGDQYIFAY
                     DMNGTVRALPFQQGLIGQNRLDIRDAHGVAFIRALVGAASRGGGHVYYVYPNPAAGFA
                     EELKVSSVMPVDDEWFIGSGIYLSHVNATFGRQEKDALMQRVHAARDFAQREGMDAAL
                     AAFNDLSGQWAPGGSYIFSYAMNGTTLALPHQPELIGTSRLGFLDHYGVAIIDWEIEV
                     ARGGGGFVYVVYLNPDTGSDALKLCYVVPVDREWFVGSGVYGAEV"
     gene            complement(9890..10813)
                     /locus_tag="PKM87_02160"
     CDS             complement(9890..10813)
                     /locus_tag="PKM87_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ28900.1"
                     /translation="MHYVVTGGAGFIGSHLCEVLAHAGHHVTIIDDLSSGRMEHIAPL
                     LPSPRVSFIQDTVQDFPSLLEYLHGVDGVFHLAAMVSVPRSIAHPLASHEVTLTGTLN
                     VLLAARDSGVKRVVHASSAAVYGNLPDLPKGEDMPVDPLSPYAVAKYAGERYCRAFSL
                     LYGLQAVSLRFFNVYGPRQDPRSDYAAVIPRFIARIREGKAPLIYGDGGQTRDFVYVK
                     DVVTALIHAMESEVCGVFNIASGSETSVNELASMLMDLFDCRLDPIHCPERPGEVRRS
                     VADISLARTVLAFSPQFSLREGLSDTLASMK"
     gene            10938..12155
                     /gene="ilvA"
                     /locus_tag="PKM87_02165"
     CDS             10938..12155
                     /gene="ilvA"
                     /locus_tag="PKM87_02165"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HNQ28901.1"
                     /translation="MVSLAEIQAARSLLEGKVIQTPLVFSPTFSEVTGADVYLKLETM
                     QKAGSFKVRGAANRIFGRRGEIGKGGVIAASAGNHAQGVAVAASMAGVAATIVMPEWV
                     SLSKQEATVGYGARVILRGSSLEESIAYARKAALEEDMTFIHPYDDPLVIAGQGTIGL
                     EILEDLPDTDLVVVPVGGGGLIAGIATAVKSSRPGARVVGVQAAACPSAAEAFREGGP
                     ITVETLPTIADGIRVKRTGDLTYPVIRDLTDGVVLVGEQAIMEAMLQLLERKKVLSEG
                     AGAAPLAALLSKGVVAAKGEKVVLVISGGNVDTFLLERILKKGLYASGRMVQLSVPVE
                     EGAHSLPALLQILAREGGVINRIDQERSAPDLPVHQMRVILELEVRGRPHLKALIVAL
                     HEAGFPARPFPAD"
     gene            complement(12412..12975)
                     /locus_tag="PKM87_02170"
                     /pseudo
     CDS             complement(12412..12975)
                     /locus_tag="PKM87_02170"
                     /inference="COORDINATES: protein motif:HMM:NF027889.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide 5'-hydroxyl-kinase"
     gene            complement(13042..13494)
                     /locus_tag="PKM87_02175"
     CDS             complement(13042..13494)
                     /locus_tag="PKM87_02175"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ28902.1"
                     /translation="MTGTETRSLTVPATLEHLEALTGMVTGFMREQGFPEKTVFEMDL
                     AVDEACTNVIRYAYAPGEGEMTVECTVSSTCARVCITDSGHPFDPLAVKAPDLTGGVE
                     ERPIGGLGVHLIRSLMDMVTYEYAAGKNILCMTKSREGVPSGSESDHL"
     gene            complement(13491..14855)
                     /locus_tag="PKM87_02180"
     CDS             complement(13491..14855)
                     /locus_tag="PKM87_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNQ28903.1"
                     /translation="MVSELVTASIVLFQMMCVVVVFAYLVTRTRFYSEVLEGTFSWKN
                     QAILILVFGIISIYGSESGIVILGAPLNIRDLGPMVAGFVGGPVVGLGAGLIGAGYRM
                     TMGGFTMVACSLATVLAGLLAGLINLANRRRFPGTLLAVSGAVLIEGMHMGLVLLISR
                     PLSQAVELVSDVAFPMIAANAAGMFIFAFITVNLENERRTCRERDRFQAELQRKNAEL
                     QIAREIQESFLPPSLPALPGYTLAAESRPAREVGGDFYDVIASPGGRVGLVIADVSDK
                     GVPAALFMALSSTVMRASAAWHQRPRDAVSDANALICREAGSGMFVTLFFCILDPSEQ
                     TLTCVNAGHNPPLLARADGSLVELTMNGIALGADSGATYEEQKVALCPGDMLVLYTDG
                     VTEAEDPSQQQFGDDRLAELAKSERARTASQALAAIQAAVAAHTRTAPQYDDMTLLVL
                     KVGS"
     gene            complement(14895..15248)
                     /locus_tag="PKM87_02185"
     CDS             complement(14895..15248)
                     /locus_tag="PKM87_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNQ28904.1"
                     /translation="MSTILQVVEERRGVVPVLFLKGRMDATTSPEAEAHINRLIDAGE
                     HHLVVDLSGLEYISSAGLRVLLAGLKRLKQCGGTMKLSALGPEIRKVFDIAGFNRLFA
                     IHGSLDEALGAGPAT"
     gene            complement(15767..16729)
                     /locus_tag="PKM87_02190"
     CDS             complement(15767..16729)
                     /locus_tag="PKM87_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNQ28905.1"
                     /translation="MQGGFFSEWLNRYRHYLRMRNYSPRTIQSYEQVVKHFAYYVWLR
                     RNIDPQKLMVYWEDFSKARLETSVDVEPVLLEDYLSFMTSMRTYKPKTLHRIISTLSS
                     FYRFLYSQGAVSANPMVAVERPKIKDQELRYLKHSQVLRLIDSVEDQRDRLIVRVIYA
                     TGVRVSELCDMNIEDIDFEDGTIRVRGKGGKIRIVFVDPDTLGEMDAFIEKRIEGPLF
                     VGQQGHHISPRAVQHIFRKYAPDGITPHKIRHSYASELYRRSKNLRVVQENLGHTSIK
                     TTEIYLHTDLEERKRVYQEFFPLSGRKDDEEKRGQKPRQVNEQG"
     gene            19668..20777
                     /locus_tag="PKM87_02195"
     CDS             19668..20777
                     /locus_tag="PKM87_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ28906.1"
                     /translation="MITVLFVDDEPALLDVSRLYLEKTGDLKVDTCFSTEQAIEMLKE
                     RSYDVIVSDYEMPGIDGIEFLKLLRNLGDSTPFIIFTGRGREHVVIEALNSGADFYLQ
                     KGGDPKSQFAELIHKIRLAVERQRIERSLQITREAVEKASVRIFWLDSRGRFIYANEA
                     TCSALGYTHEELMRLTIGDIDPFFSRDEWNRFTERLRSHRSMVLQAFHTRKDRMKISV
                     EMAFNIGEIDERGIIFAYSWNARERTALDVLAAQADARCHRIAEAFPHLYFEVEADGR
                     LRFWNNAASAFTGQEYKDRTHMGGTFLDIVMKGQQAHVANTISRVGEGHQDEVIQVPL
                     TGHGDMRRNVIFRARGFEGEHGGHFVQLLEIPPES"
     gene            21145..21711
                     /locus_tag="PKM87_02200"
     CDS             21145..21711
                     /locus_tag="PKM87_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5806 family protein"
                     /protein_id="HNQ28907.1"
                     /translation="MTQYGQEDVNKYRKFKKVDGATYRKVNQFLRKRTYVTAREWAIA
                     RLCTDFRTRSGAEMTFIGQHLPELVPFMQECYTPQAVNQARNSFKKKVYKSGATFFYG
                     AMCGFFTSEELDDLLFEASEVARFLLEVERTSLDIDEEIEIEDRITDVMRSVGEAAAQ
                     ILRARMHNEDQTGGEPEVAGNPPDQEQV"
     gene            21708..22466
                     /locus_tag="PKM87_02205"
     CDS             21708..22466
                     /locus_tag="PKM87_02205"
                     /inference="COORDINATES: protein motif:HMM:NF015181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNQ28908.1"
                     /translation="MRVIHIAGVSGSGKTTFIRSLIPLLEQKGPTAVAKHLGHHCYSL
                     EKGKDTTIFMEEGAHVSAGVDIEKTVVVARGLSFGDILPLLSSIGTEYLLVEGWKTQP
                     LPKVAIGDLPGAAEVILSNPTAAEVIASLDLFPRYYSLEGLAQKVQEGCTGGGVVLTG
                     RYPVPDHAGNPESRREFYLRFSPTLHEISRVAESRPGGVRTMVHLHQGLLFGGEDGIL
                     VAVGAPTPADALDAFSSCHRHLLSALGTGSPHQG"
     gene            22469..23824
                     /gene="glmM"
                     /locus_tag="PKM87_02210"
     CDS             22469..23824
                     /gene="glmM"
                     /locus_tag="PKM87_02210"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNQ28909.1"
                     /translation="MEGQKLEKKLFGTNGVRGVAGREMTPALALSIGAALGTMRQGTI
                     AVGRDTRTSGPALACALKAGLLSAGCDVVDLGVLPTPALQYLVRDHFDAGSVVTASHN
                     PPEYNGIKVVEPDGTEMGDEQSILLEDHIFRGTAYQAAWDHVGREHAAPYMVEDYVRA
                     VAGSFPTGTGGGTLVAADPGCGAAAWTTPEILTRMGCRVFSINSQMDGTFPGRLPEPS
                     PEGLAPLADLVKATGARFGVAHDGDADRAVFIDDKGRFIEENRQFGLIADFICRNRRG
                     VVVTPVSTSRLIEVIAHRHGCSVVYTAVGSIYVARTMIGLSKQGTAVAFGGEGNGGLI
                     FPAHQLCRDGGMTAATMVALLADTSQPLSTLVDALPPFFMMKEKLQEKDAPGLIRRLK
                     DAYRGESLDLTDGIRINREDSWALVRPSGTEPLVRVMVESTSKTGARQFHEELMAHIR
                     Q"
     gene            24289..24573
                     /locus_tag="PKM87_02215"
     CDS             24289..24573
                     /locus_tag="PKM87_02215"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNQ28910.1"
                     /translation="MKTVEIRISGRVQKVGMRNCVRHIAGKLSIRGDVMNQADGTVRI
                     RATGDPIILDKFVSMLYSCPRALIRDIEILDIQFAQFPDFAVVRTAEETR"
     gene            complement(24583..25527)
                     /gene="cas1"
                     /locus_tag="PKM87_02220"
     CDS             complement(24583..25527)
                     /gene="cas1"
                     /locus_tag="PKM87_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HNQ28911.1"
                     /translation="MEWSVIEPVPWYLVSGFGAHLKATPRTLTVQKNGHTEELPLSRV
                     RHLLVMGGHHIHTSVVARLLASGSCISFFEADGEPLGIAKPFGFRYDEVVREAQAKAY
                     SHSFALAVAKGSIHSRIQALVGLQEARDEPLFYEGELEILEKSYAELEFLVRVEELRR
                     VHRLASDMYYEILARTIPQETGFRRRTARPHVDVVNTMLSFGYGLLFGNACVAAIGAH
                     LDPDLGFLHKGKGGLVYDIIDPFKPAMVDGMVSAIAREGIPEEEYECGPSRCILSDDL
                     LVVLTKALHETIRQEVIDEQVMALQGALIKKNDYLVIT"
     gene            25825..27114
                     /locus_tag="PKM87_02225"
     CDS             25825..27114
                     /locus_tag="PKM87_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNQ28912.1"
                     /translation="MFWDEKTETLPKRDLEELQLTRLKKTIAQVQNVEFYRKKLKEAG
                     VRPDDIRTLGDIEKIPFTRKQDLRDGYPFGFLAVPLQQVVRIHTTSGTTGKPTVVGYT
                     RSDLETWAGLIARNMTMVGLTDRDIFQNMVNYGMFTGGLGFHYGAEKVGMTVIPSATG
                     NTRRQIEMIQDFGVTAIHCTPSYAMHLAEVAEEVGAHLDSLSTGLFGAEPWSDSMRAE
                     LEKKLELTAFDSYGLSELFGPGVAFECPERDGLHIWHDCYLVEIIDPVTGETLGDGER
                     GELVVTPLVKEAMPLLRYRTGDITMRFEEDCLCGRAKKIARITGRTDDMLVIRGINVF
                     PSQIEHVLLRIPEVGNQFMVYVDRINHLDEMTIDVEINRDYFSGELQDLARIQKKVVK
                     ELRDTLELRTTVKLVEPGSLPRFEGKAKRVIDRRGEM"
     gene            27114..28415
                     /locus_tag="PKM87_02230"
     CDS             27114..28415
                     /locus_tag="PKM87_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNQ28913.1"
                     /translation="MRAWDPRIEEMPKADLEVMQYRLLKTLVYRLYSFSPFYHRRMEE
                     NHVHPDDIRSLGDIRKLPFMYKRDLRDNYPDGLFVSTQEDLVRYHVSSGTTGKPTVVG
                     YTERDLENWTASLARGLVSCGLGRGDVIQVSYGYGLFTGGLGLHYGAERIGATVLPTS
                     VGNTERQIELMQDLKATAIACTPSYLLHMGEVAEKMGVSIKDDTRLKVGILGAEPWSE
                     QMRQRIQDWLGIRAYDIYGTSELSGPMFCECSEQKGFHVWGDLAYVEILDPDTLEPLP
                     AGERGEMVITMLQKEALPMVRYRIGDISAIDDSVCACGRTHPRVQRITGRVDDMLIIR
                     GINVFPSQIEYALMSIPEVGQHFQIIVDRKGALDEMLVQVEVKREAFSDKITDLMSIR
                     KKVEHKLRNILNVAVNVELVEPGSLPRFEGKAKRVIDRRKI"
     gene            28417..28851
                     /locus_tag="PKM87_02235"
     CDS             28417..28851
                     /locus_tag="PKM87_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNQ28914.1"
                     /translation="MKESRFVIKQISIFSENKPGRLAAIARALQEEKINILAFSIAEA
                     DGFGVVRALVDKPKKAHEKLGALGFNVAFTDVIAIRMKDEPGGLYETARILGEGHINI
                     EYAYAYSGKDAAVLILRVDNVEAAISAIQKGGGTLLESSLFQ"
     gene            complement(28858..30144)
                     /locus_tag="PKM87_02240"
     CDS             complement(28858..30144)
                     /locus_tag="PKM87_02240"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HNQ28915.1"
                     /translation="MKRTILQVALDITELDRAVQIAREAVEGGADWIEAGTPLIKSEG
                     MEAVRRIRALFPGREVVADMKVADTGALEVEMAAKAGASVVCILADADDSVIAEAVRA
                     SDLYGVRLMADLINVRDPILRARELEQMGVHIINAHVGIDQQMIGKSSLSMLASLRAS
                     VNVPIAAAGGLDATTAPQAVIGGADVVIVGGAITRSATVTESARLIREAIDSPTLPRE
                     EQVSPDQAIRDILRVVSTPNISDAMHRKGAMAGISPLCRDVKMAGKAVTVQGFAGDWA
                     KPVEAIDVAGQGDVLVIDNQGVTHVACWGELATMSAMERGLAGVVIDGAARDVDDIRK
                     MRFPLFSRAVVPNAGEPKGFGEINAEIRCGGQVVRPGDWVAGDESGVVVIPRERAMEI
                     ARRAMEVKKNEERVREEIRRGSTLSRVSDLIRWEKK"
     assembly_gap    30326..30425
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(30980..31489)
                     /locus_tag="PKM87_02245"
     CDS             complement(30980..31489)
                     /locus_tag="PKM87_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNQ28916.1"
                     /translation="MNVGTTIIKSRHSVRKYKPDPVDETTIRDVLECARHAPTAMNLQ
                     PWLFGVVRDRDLLKKIADLTDHGKFIADAAICIAVFGEKKAKYYLEDCCAATETLILG
                     LQSYGVGSCWVAGEKKEYAEAVRKLLDVPGNYTLVSLVPAGFPAEIAIQKKKDLDDIT
                     FSDKYKEQQ"
     gene            complement(31554..32546)
                     /locus_tag="PKM87_02250"
     CDS             complement(31554..32546)
                     /locus_tag="PKM87_02250"
                     /inference="COORDINATES: protein motif:HMM:NF024270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3821 domain-containing protein"
                     /protein_id="HNQ28917.1"
                     /translation="MKRSNEGHYNYKAILALAVLLILVAPFSAAENTIPQGGTVFVGE
                     EDLDITLTGATAGSSLAWYGTGGQVSHAPAAQVTVGDPSAFYVSPITFGGKTGPWFLL
                     PENTVAFYLEEPSLGIRVVDYSSGFVVSPSATWIPKGDTAGFRVDTNLWVMANRPGTA
                     GAPLSIRLNGPDSLTFSSLGGYSLEGVVVSSSPFETGPVWATGSAEYPIGEYSVYVRC
                     DANDMSDNYPAAGKAISEKVTFLVQRVNPLITGTTPLPVTSVPTATRTPTPAGSPPAS
                     PIETATTATPTVQETGTPLPPTPTPTPAPGFLGFAAVLAFLAALALLVRKGRMP"
     gene            complement(32620..33822)
                     /locus_tag="PKM87_02255"
     CDS             complement(32620..33822)
                     /locus_tag="PKM87_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNQ28918.1"
                     /translation="MTGELRIVGTAHVSAKSVEEVRAAIEEFSPDVVAVELDPARYAV
                     IRKQAKDPSVQDILEAKNFTQILVQWILAYLQRRIGLDLGVEPGAEMKAAIEEAESRG
                     IAIGLVDRDINITLRRFWRSLSFFEKLKMLYALAVSVASIDGEEIDVEQLKEQDMLTA
                     AMEEFRKFSPNGARALIDERDAYLAHNLAGLTTRHARVLAILGAGHVEGVRKYLDAPG
                     TLPPIQQLTAEVKSRPWGLIFGVAVTVLFVLLLLALAFSGVGFEVLGMALVYWVLVHG
                     ILTAAFTLAARGHPLSALTGFGVSWFTALNPLIAAGWFSAIVEAKIRKPAPADFRRIF
                     EAESFSEMMKVPLFRVVLVAALANLGSTLGTVLYFMFIFPVLGIDPGVLITQGFSNMW
                     NAITGIFS"
     gene            complement(33819..34370)
                     /locus_tag="PKM87_02260"
     CDS             complement(33819..34370)
                     /locus_tag="PKM87_02260"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNQ28919.1"
                     /translation="MKENESSMCEETLAARLQEIGFSCTRCGECCRAGNGDDNLVMVS
                     PAEVRRISAHCRLDAAEVAEPYPESVEISGGRRITFGWAIRRISGDCMFYEGGSCRAY
                     ATRPWICRTYPFMLDGDTLLTFPCPGLGSPVGPEEARALARDLLARRAAEQEQEERIR
                     RAFSMLPQGEGRLIVDGEGVKGV"
     gene            complement(34367..34930)
                     /locus_tag="PKM87_02265"
     CDS             complement(34367..34930)
                     /locus_tag="PKM87_02265"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase subunit A"
                     /protein_id="HNQ28920.1"
                     /translation="MLPIFVVNNHGQFNHLILRMFRDLDIDARMVPNTTPPEELAGAC
                     RGIVLGGGPDMGRSGLAGRYLHLGLPVLGICLGLHIIAMEFGGRVQPGRSGGYGPVQV
                     EVVDAGEILSGYPETISVWASHADEVAEAPPGFRLLARSAICPHEAIARPDLHLYGLQ
                     WHPEVSHTCDGLRVYENFDAICREQGA"
     gene            complement(35085..36128)
                     /locus_tag="PKM87_02270"
     CDS             complement(35085..36128)
                     /locus_tag="PKM87_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00303 family protein"
                     /protein_id="HNQ28921.1"
                     /translation="MRPRLLKKSVSMAFLSKDPGIAAENPLFCSILANTMLSVVPGIS
                     GAGPTPEKTLLTPVLDAELIAKGAITSVPARPDTPTGCPTPASITRSIMELCGTRALF
                     INAGLSHLPTVPCMDVYGEAGNDPRFMDAVPRAEALFQSGESLGEFLSRMCDLLIVGE
                     CVPGGTTTALCVLRALGYPARVSSSFIENPVKMKEEVWNAVAGRIRAEGVESPMDILR
                     CCGDPMIPVCAGMGSTCTRRIVLAGGTQMLAVAAVMKAMEIPLPLVATTAYVRDDPSA
                     NFCEIAERIGVEVVYVDPGFGDLGHSGLARYCIGEVKEGMGAGGAMLMAYLLGNSPSA
                     IRAKILETVASYH"
     gene            36212..36646
                     /locus_tag="PKM87_02275"
     CDS             36212..36646
                     /locus_tag="PKM87_02275"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNQ28922.1"
                     /translation="MFEIGERLRQKGAGMDEIVDAAMELYVPHGVSAGEARDLIRKMI
                     HKYMDDPNIASLLLGAVLLEDELYWKRVNSEIKDDPVFLLSDEIIGMAIAECIGGTYA
                     RFEFTRYDQKKPGILGALGPFLDDAVAGLIAGCTSRFYSECH"
     gene            36637..37353
                     /gene="cobS"
                     /locus_tag="PKM87_02280"
     CDS             36637..37353
                     /gene="cobS"
                     /locus_tag="PKM87_02280"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNQ28923.1"
                     /translation="MPLTALVSLLQFTTVLPLGRTRDFSAFAERSWIYPLAGYVVGGI
                     AAAAVFFIGEPMVAGALAIALLFFLSGCNHLDGLMDFGDGLMAHGGREKRVGALTDRQ
                     IGTGGVAMAIAVTLLSFGSLAAAPCLPCALIVAEVGGKFSMAFLSTFGRPFREGIHSS
                     IQSHSKPYFPVLAAILCIPLVLLPIAPASLGLASVMMIACPTALLLVSRKLFGGVNGD
                     VTGASNEITRAAVLVSLCLL"
     gene            37398..38297
                     /locus_tag="PKM87_02285"
     CDS             37398..38297
                     /locus_tag="PKM87_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ28924.1"
                     /translation="MRRENIVHMRGGVITERDPEFCTIRTRIPAGVVTPAMLRGIADI
                     ADSCQAPHLHLTTRQTVEIPRVDPGRLEEISRALGENGTPIGSERDEVVNITACPGID
                     RCVFANIDSISLAVEIDRRLFGREMPVKVRIAISACPNACTSPMLNEVGITGRIMPIR
                     TPGVCTGCGTCVEYCKEEAISIKKGISVLDPSKCVQCGTCIRSCPFHLLKSSGEHYLI
                     SVGGRRGRHPRVGRELITVNSPGAAADVVEKVVQWVYRRAWSGRLLSEQLDEIQFEKF
                     REEIRSQIPASSIFEAQDPDQSR"
     gene            complement(38285..38587)
                     /locus_tag="PKM87_02290"
     CDS             complement(38285..38587)
                     /locus_tag="PKM87_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28925.1"
                     /translation="MKTLPITKDASGNRATVECATGEVSVHKFCAFCEHCKGIKVGPR
                     VYPAPQEQVQKEMKRGSASDEALMVAALQFNQLVRDGTAIECADDQSQGFRPRYRL"
     gene            38766..40043
                     /gene="tuf"
                     /locus_tag="PKM87_02295"
     CDS             38766..40043
                     /gene="tuf"
                     /locus_tag="PKM87_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor EF-1 subunit
                     alpha"
                     /protein_id="HNQ28926.1"
                     /translation="MAAEKPHMNLAVIGHIDHGKSTTVGRLMFETGTVPAHIIEGYRK
                     EAEAKGKATFEFAWVMDNLKEERERGITIDIAHKRFDTPKFYFTVVDCPGHRDFVKNM
                     ITGASQADAAILVVAAPDGVMEQTKEHVFLARTLGIGQLIIAVNKMDAAKYDQKRFEE
                     VKKDLSALMQMVGYKPSETLFIPISSLAGANISKKSADTPWYTGPTLLEALDTLKEPE
                     KPVDKPLRLPIQDVYSISGIGTVPVGRVETGVMKKGMKVSFMPANKEGEIKSIEMHHE
                     EIPQALPGDNVGFNVRGIAKGDLRRGDVCGPVEAPPTVAEDFTAQIVVLQHPSAITVG
                     YTPVFHCHTTQTACTFIELKKKLDPRTGQTKEENPTFIKTGDAAIVQVKPSKPMVIEN
                     VKELPQLGRFAIRDMGSTIAAGMCIAITPKQMR"
     gene            40077..40385
                     /gene="rpsJ"
                     /locus_tag="PKM87_02300"
     CDS             40077..40385
                     /gene="rpsJ"
                     /locus_tag="PKM87_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNQ28927.1"
                     /translation="MQKARIRLTGTDFKKVEMVCDRIREIAERTGVNLAGPIPLPTKR
                     LVIPIRKSPDGEGTATWDRWQMRVHKRLIDIDADERALRQLMRTQVPKDIGIEIVLES
                     "
     gene            40396..>42025
                     /locus_tag="PKM87_02305"
     CDS             40396..>42025
                     /locus_tag="PKM87_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HNQ28928.1"
                     /translation="MTAAGIAQRIRPLFSFHTLFFLIVLGGFLLRFLALDLKLLHHDE
                     AIHAWFAYELLTKGTYIYDPMYHGPLLYYVTAGMFYVFGQSDLVARLLPALFGAAIIP
                     LVYAIYRLGYLDQRQALIAALFIAISPDLVYFSRFLRHDIFQLFFTALILVALLAYLE
                     RGKVWYALLAGLAAGGGMCLKEDMPLFLVIVALFGIYLLWKQPLPIPRTWKRDIVLAV
                     LLAVAIMAAFYSSFGVHPEILVTGLEKAYTHWASMHGMCRICGPWFFYILLFLLYEVP
                     VFLMAIFGMVQFADRHNPVPAWIARARAFLAARRPANQEESEAVMTPRSASVPVAWDK
                     KEIFFLFCILWFLAALGAYAYIGEKVPWLIIHQLLPAIFVSVYLMSKKKAVFALAGCV
                     FLVLMTWHVAFIPADVNEPIVQVQNSEDMRLVMALIDVSDSVAIASENYWPLPWYYRG
                     EGWSRMHFYGKIVDEYTIYQVNPDMVITHDQASYASLDGYEKHTYKLSYWFSIYDNEN
                     RLPEYYVKRDGKMGSINIDVFTKPGLYQKAGIVPP"
BASE COUNT         8804 a        12726 c        12157 g         8138 t
ORIGIN      
        1 gcaacaacct ataaaactcc tcaggccaga cctcaatagc agcgaattcc catgaaaggc
       61 atcatcctcg ccggcgggtt cgggacccgc ctctaccccg tcacgaagtc tgtctcaaag
      121 cacctcctcc cggtgtacga caagcccctc atctactacc cgctctcggt cctgatgctc
      181 tccggtatcc gcgagatcct gatcatctcc acggcgaggg atgtccccct ctaccgggaa
      241 ctcctggggg acgggcgcca ggtcgggctc tcgttctcgt acgccgtcca ggagaagccg
      301 ggaggcctcg cggaggcctt catcgtcggg gaaaaattca tcggcaccga tgccgtggcg
      361 cttgtcctcg gggacaacat tttctacgga cagcacttct cggaactgct gaggaaggca
      421 gcctcgcaga agcacggtgc cactatattc gggtactggg tccgggaccc gaggccgtac
      481 ggcgtagtcg aggtgggacc agacggccag gcactctcga tcgaggagaa accggcaaaa
      541 ccgaggtccc actacgcagt ccccgggctg tacttctacg acaaccgggt gatcgggata
      601 gcaaagtcta tctcaccctc tgccaggggg gagctcgaga ttaccgacct gaacagggaa
      661 tatatgaaga agggcgagct cttcgtgctg gtcttcggga ggggaatggc gtggctcgat
      721 gccgggaccc acgactcgct cctcgaggcc ggaaactaca tcgagaccat ccagaagcgg
      781 caggggctct acgtggcctg catcgaagag atcgcgtacc ggcaggggtt catagggaag
      841 gaccagcttc gggcgctcgc agacggtctg aagaacacgg agtacggacg gtacctggca
      901 gagcttgcag agaatgagtc cgagatgcaa taacgcaggg gggagatacc gctgtgtcac
      961 cctgtgattg ctctgcagcg ggggcctgaa gataaaaggc ggggcagata atggagatat
     1021 tctcattcgg atgtggagta tactcttata ggggaaccgc aaaactgtaa acaggaatga
     1081 aatcgaggct ggaaatcctt tcaatactga ataatctcaa acaggagctg tcccctcgtt
     1141 accatatccg aaggctcggg atcttcggtt cggtcgcgaa agacgctcac actccgactt
     1201 cggacgtgga tattctcatc gagttcgatt acccgataag tggattctcc ttcgtgcatc
     1261 tcaaggaact cctctcttca tctctcgaaa tgaaagtgga cctcgtgact ccgggaggac
     1321 ttcatcctct tctcgcagat tctatacaga gtgatgtgct gtatgtctga gaagagagcc
     1381 tacgcactct ttttatacga tatggcatgt gccgtcgata aaattctcga atataccaaa
     1441 tctctctcaa gacagaatct tcttgagaac gaactcgtga aggacgcgat ccttcgtaat
     1501 atccaggtcc tcggggacgc agcaaagaag gtcccgccgg aattcatcgc atctcaccag
     1561 gagattgact ggaaaggaat cgcaggactc cgtgatatta ttactcacca gtattttcgg
     1621 gttgactgga acatactctg gactaccatc caggaagacc tccctccact ctcctctacc
     1681 ctccatagtc tgcttgaaag atccttggca gatgacaacg gacgaagcgt atgacatccc
     1741 caaaattttc tcccatcccg acctctcttt cgggcgctgt gctgatcgag ccccgcgtct
     1801 tcggcgacga ccgcgggttc ttcctcgagt cgtacacgag aagagacatg gcagcgctcg
     1861 gcatcccgga agagttcgtc caggacaacc actcgcggtc ggcgaaaggg gtcctccggg
     1921 gactgcactt ccagtgcccc ttcgcccaga gcaaactcgt ccgggtggcg agaggggcaa
     1981 tatacgacgt gatcgtggac atcaggaggg gctcgcccac gttcgggcgc cacgagtctt
     2041 tcctgctctc cgaggagaac caccggctgc tctacgtccc ggtgggattc gcccacgggt
     2101 tccttgcgct cgcggaaggg accgagatcc tctacaaggc aggcgaatac taccaccccg
     2161 aggcagacgc cgggatacgc tggaacgacc ccgacctcgc aattccctgg cctgtctcag
     2221 agcatgggat cgcggcaccc cttctctccg acaaggacgc acgcctcccc cttttttcgg
     2281 acatcgagag cccgtttctt taccaggggg caagcagatg agtcttcctg aagagtccct
     2341 cctttccctc ccggtggaga gagaaccacc ctctccgcat cactcccata tccacgggtc
     2401 aggaatgcgg aaaagtgaga gaggtccccc gcgtatcacg accgatcgat ccgccaccct
     2461 ttttcctggc agcatctccg ggagggcaca ccaatgaaag tcatgatcac cggtgcagga
     2521 gggcagcttg gccaggagat gacaaaacag gcagcaacga ggggatacga taccatcccg
     2581 ttcacctcac gcgagctcga catcaccgtg gcaggcgccg tccgcggggc catcctccgc
     2641 caccgccccg acgcggtcat caactgcgcc gcgtacaacg ccgtcgacca ggccgagcgc
     2701 gactggaagc gggcgtttct cgtcaacggc ctcgcggtgc gaaacctcgc gctcgcggca
     2761 catgaggcgg gcgccgtcct cgtacactac tcgaccgact atgtcttcga cgggctctcc
     2821 ccccgcccct acaccatcgc cgaccgcccg aaccccctca gcaggtacgg ggagagcaag
     2881 ctcctgggag agcaggcggt ccgcgaccat tccaggcgct actttcttat ccggacctcc
     2941 tgggtctttg gacccgggaa caccaacttc gcgcagaagg tgatcgagtg gagccgccag
     3001 aacgatcgcc tgaagatcgt ggacgaccag gtctcttcgc ccacctgcac gcgtgacctg
     3061 gcagacgcaa ccttcggcct cctggagaca gggcagttcg ggctgtacca cctcaccaac
     3121 gggggatcgt gctcccggta cgaatgggcg aagtacatcc tcgatgcaac cgggtggaag
     3181 ggcgtgctcc tccctgcgaa gagcgaagag ttccccaccc ccgccctgcg gcctgcgtac
     3241 tcggtcctcg acaacttcgg ggctgccgag gtgcggggac aggagctccc tccctgggag
     3301 gacgcgacgc gtgcgtacct cgaagagata ggagggattc catgacgcat atccttgtca
     3361 ccggggtcgc cgggttcatc ggcagcaact tcgtatacca cgtcctcggg acccaccctg
     3421 agatatccat cgtaggcctc gactctctca cgtatgcagg gaacctcgag aacatcgcgg
     3481 cgcttggaag gaaagagcgg gagcggttca ccttcgtgcg gggagacatc ggcgaccgcc
     3541 ctctcgcaga ctcgctcatt tcggagcacg cgatcgatgc cgtcgtcaac ttcgcggccg
     3601 agtcccacgt tgaccgctcg atcgaggacc cccagctctt cctccgcacg aacatcctcg
     3661 ggacctgcac cctcctcgac gcggcaaggg ccgcctggca acggaacgga cgcatgaccc
     3721 ccggatgcag gttcctccag gtctcgaccg acgaggtgta cggatcgctc ggcccagagg
     3781 gatatttcac cgaagagact cccctcgacc cccacagccc ctactctgcg agcaaggccg
     3841 ccgcggacat gatcgcaaag gcataccacg acacattcgg gatgcctgtc ctgatcaccc
     3901 gctgctcaaa caattacggc ccctaccagt tccccgagaa gctgatcccc ctgataatcc
     3961 gaaacgccct ccaacaccgc ccgcttccgg tctacgggga cggaaagaac gtcagggact
     4021 ggctctacgt cgaggatcac tgccgggcga tagacgtggt cctcagatcc ggccagccag
     4081 gggaggtata caacatcggg gggaacaacg agtgggagaa catcgcgatc gtgagactcc
     4141 tcctttccat cctgcaggag gagactgggg accccgggat caacgagagc ctgatccagt
     4201 tcgtgaaaga ccggcccggc catgaccagc ggtatgcgat caactcgacc aagatctccc
     4261 gggaactcgg gtgggagcct gcaaccccgt ttcctgacgg gataaaaaag accgtccgct
     4321 ggtacctgga ccaccaggac tggatggaga gagtgatctc gggggaatac cagtcgtttt
     4381 acgagcggaa ctacgaacac cggtgaacag gtgtggtccc atcccacctg gccacacgga
     4441 aacgcgagcc ggcatgaact cctgactggc acagggaggg aagagcccgc atcgaccatc
     4501 cggggcggca gtccctcctc ctccttttta tccttcagtc ccctaccttc agagtgatca
     4561 tgatctgcct cgtgctcggc acccgccccg aggtcatcaa actctccccc ctcatacggg
     4621 agtgcgggcg actggagatc ccgtactacc tcctccatac cgggcagcac tactcgtacg
     4681 agatggacag ggtctttttt gaggacctgg aactgccggc cccgcagttc aaccttgaca
     4741 ccggctcggg cctgcacggg gagcagacag gaaagatcct tgcgggcgcc gagcgcgtct
     4801 ttatggagca ccgacccggt gcggtcctcg tccagggcga cacgaacacc gtgcttgcgg
     4861 gagcgctcgc ggcagcaaaa ctccatgtcc cggtcggcca cgtcgaggct ggcctccggt
     4921 ccttcgaccg ccggatgccc gaggagatca accggatcgt cgcggaccac gtgagcgacc
     4981 tcctcttcgc acccacagag accgcagcag gataccttcg cagcgagggg ataccgggcg
     5041 agaagatctt cgtcaccggg aacaccgtgg tggacgcagt cttccagaac ctggagattg
     5101 cgcggaagaa gaccggcgtc atggagcgga tgggagccaa agagggtgac ttcatcctcg
     5161 tgacagccca ccgggccgag aacgtcgacg accggggccg gctggcagga atcctcgaag
     5221 gcatcggggc agtgggagag gagaccggca tgcaggtcat cttcccggtc cacccccgga
     5281 ctgcgaagat gatcggggag tttgggctgg acaccccggg aatccgcatc gtcccgcccc
     5341 tcgggtatct cgagttcctt ctccttgaat caaaggccgc cctcatcctg accgattcgg
     5401 ggggcatcca ggaggaggcg tgcgtgctcg gcgtcccctg cgtcacgctt cgggagaaca
     5461 ccgagcggcc agagaccctc caggtgggat caaacgtgct cgcggggaca aatccaacga
     5521 ccatccggga gagcgcaaga gagatgcgct cccgggaccg cggctgggaa aacccctacg
     5581 gcgacggcca tgcaggggag cggatcatcg ggatccttgc caccatgggg ctgacgggat
     5641 gaccacgggt cgttgccggg accagaagga ttcgtatccc cacctggggg ccgtggcgcc
     5701 atggaccggc atgagggcag gcctggtcac cggtagccgg gtgagggtcc gcgatacgcc
     5761 ccgcaacaat gtaacacgcg cattctcccc ttctctcccc ttccagcaaa gggttattcc
     5821 catgagatcc gttcgctttt tcgtgctctt catcctcgta tgccttgccc tccctgccgc
     5881 tgccgtaacc atcacggaga gacccgacac gatcgcaaac ggccagccga tattcgtgac
     5941 catccgcgat ctccccggaa actcctcgtt ctcgctcctc gtgcaggcag actgcgccgt
     6001 tgaacccaac accgagttcc ggttccagat gagccagttc gtgatgccgt ttgcgttaaa
     6061 gaagagcagc atcacggcga ccctctcggg aacctccacg aaccgcctgg aagtgatgaa
     6121 ggagtcgacc atcgtcacgg tctcgggcca gtccagggat ggccaattct ccaccaccaa
     6181 gcattacgac atcactccag ggacctacga ctacttccgg ctctcgggga cctcccttcc
     6241 gacagcaagg agtatcaccg cacagctcca ggtgaccggg accacgcagg ggccaaagga
     6301 ctccgagata tcgttcgtgg tcgagggcct taccgccggg accatcacca ttgcagccct
     6361 cgtcgacggg acgcaggtcc tttacaagag catcccgatt ggggtgtcgg aatcgtcctc
     6421 tgcaggggac ggcgtgcctg tggccactac tgcacccccg ccggagcagt ggaacacgtt
     6481 cacctcagcc gacggcctgg tcagcgtcac ggtctcgcac gcgggaccca tcgggtttct
     6541 gaaggtccct cctgtggacg tgacttgggg gatgcaggcc atctccgggc cttacaccct
     6601 ggtgccccag gatacctctt tcctcccccc gggaagaatc gagttccgca tcccggccgg
     6661 ggctccgagg gatgggctcg tcgtcgcttc ccttgaccgc gaaacatgga ccatcctttc
     6721 ctcgacgatt ggaaacacct ccgtgacggc tcccgtcacc atcagcggca cctatgccct
     6781 gctggggccg gtcccggtcc tggcaaccac cacgaccact gcgatcacac ccattgcgac
     6841 cactgttgag gagactcccc ccacccccac gcctgcagac accacgaagg caggtatcgg
     6901 gggtgccacg cttatcctcg cgatttgtgc cgggtgtgca gtcgcgatgc ggaagataca
     6961 atagcgcatg ggtccggaag gagcagtgca tctccaccgc agcggaggtc gcctgctccc
     7021 cgctcttacc ccatctcgaa cgtggtgacc ccgaagatac ggccgaccgg aagcggaggg
     7081 atctccttcg tgtacatcct cgcggtgccg aagacctcct ccatctcccg ctcctgcgcc
     7141 attgccaccg cgtcgcggtt cggctccggg gtgtcgaaga agaacagctg ccccctcacc
     7201 tcatccctcc cggtcaaatc ctccaggata cactcgccga tttccggcgt atcggcaaag
     7261 agcgggccga tcttataccc ctcgaagcac ctccggatta ccccgtagcc ccgcagtgat
     7321 cctccctcca ccaaggcgga ggaccacgat ccgggcgtgc ggatccatcg ctccaggaag
     7381 cgcttccgtt ctgcgcggaa atggagggca tcatacgcaa cgagtgcgga aaagggcact
     7441 tcctgcgcag agccgagccc tgcctgcctc cttccgtccg cgatgccccg ccaccggatg
     7501 ttccggtacg cgaagcggaa ccccatccgg gaagaatact tttcctgcat ctcgtagacc
     7561 ccgtcgatgc ccaggttccg gtcgcccgcg tggcggattg ccgcgctcga gagctgccag
     7621 cccaccccct tcgaccgcat atcctccctg atgacaaaaa aacccccaaa agagaaccgg
     7681 gagtcgtaat tggtgaccgc aacagtcccg acgatctcgc cgtccgcctc cgcgacaaac
     7741 cacccggccg ggtccaccgc gtagtggcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnt ctcgccgtcc gcctccgcga caaaccaccc ggccgggtcc accgcgtagt
     7921 ggcattcccc gtcatgcagc cccgggttcc acccctctct cctcgcccac tcgatgaaga
     7981 ccggcacctc ctcctcccgc gccgtccgga tcacaaggtc atccatatga gaggatgaag
     8041 cggcaatgga cttatagttc agtgctgcgg ggagcagttg ggccatcggc ccccactgcg
     8101 gggaattccg tcgtgaaatg cccgtattac atatgatcca cgggtcgaac tgaacccacc
     8161 cactcacacc tccgccccgt acactcccga ccccacgaac cattcgcggt caaccggcac
     8221 cacgtaacag agcttgaggg catcggaccc ggtgtcaggg ttcaggtaca ccacgtagac
     8281 gaaccccccg ccaccgcgtg ccacctcgat ctcccagtcg atgattgcaa ccccgtaatg
     8341 gtccagaaag ccaaggcggc tggtcccgat caactcaggc tggtgcggga gggcgagggt
     8401 cgtcccgttc atcgcatacg agaaaatgta gcttccgccg ggtgcccact gcccggagag
     8461 gtcgttgaac gcagcgaggg ccgcgtccat tccctccctt tgggcaaagt cacgggccgc
     8521 atgcacccgc tgcatcaggg cgtccttctc ctgccggccg aaggtggcgt tcacatggga
     8581 gaggtagata ccggacccga tgaaccactc atcgtcgacc ggcatcaccg aggagacctt
     8641 cagttcctct gcgaaccctg ctgcggggtt ggggtataca tagtacacgt gcccgccccc
     8701 gcgggaggct gcccccacca gggcccggat gaaggcaacg ccgtgtgcgt cacggatatc
     8761 aaggcggttc tgcccgatga ggccctgctg gaacggcagc gcacgcaccg tcccgttcat
     8821 gtcgtaggcg aagatatact ggtccccgtc cacaaaccgg ccggctgggt cattgaactc
     8881 cctgagggcg gcctcttttc cctccattgc ggcaaaggcg gcggccgact gcacgtccgc
     8941 atcaaggtcg cttagtcccg ccagggaagc gttcctcacg aaggatgcat ccgcagtgcc
     9001 ggactggatc ttcccttctg tcagcaccac ccgccacgcc gctccgtcaa ttccggcagt
     9061 ctcccaggcg gcctcttttt cgatctcctt gtcccactca cggtcccaga accggtagga
     9121 gcctgttccc gcgggttcgg ataccactct ccggaagaac tcctggagtc ccggttcctg
     9181 gtagacggga tcagaaaaga ggttctttcc tatctcctcc tgcgtcgtgt cgtagatgac
     9241 cgtcccgtcc gtctgtgtca cccagatgtc ataggctgtc ccctccacca cgggcgcaat
     9301 agccctccct atgagggtct cggggcgata cgtgacgtcg gtatagccaa ggtaggtgcc
     9361 gtctgcggca aagaccgggg cactctggga aatcccggca aacccttcct ccatcatgaa
     9421 gacctcgctg acccggggca ccttcatggc cagggcttcc tggaccggac cgtcgtaatt
     9481 cacgtccctc ccgacaatcc cgcggtaatg ctccgggacc gcggcgacga ggattccctc
     9541 aggggagatg gtgaccgacg agagggccca aggaagcgcg tcgagtttct cgaagagcac
     9601 cgcctctgca tccgggcctg cgagccctgt ccgggacagc cggccggcac tttcgaggtt
     9661 gagagcccgt acatcggcga gctccgcatc gatggctgtt gcggccaaat tgagcgtggc
     9721 gctggccaca gatgcctctg tcttttcctt ttccggggtg atgcacccgc tggcgagaag
     9781 cgctgctccc aggacaagga ggatcaacag tatgcgtacc ttcatgcatt gacttttgat
     9841 caaccggccc gcataagcat tgtgaaaaga gcgacagggt gtctgcgggt catttcatcg
     9901 acgcaagcgt gtcagaaagc ccttctctca agctgaattg cggggaaaac gcgagcactg
     9961 tccgtgccag ggagatgtcg gcaacagatc gcctcacctc gccgggcctc tcggggcaat
    10021 ggatgggatc cagcctgcaa tcgaagaggt ccatgagcat cgaggccagt tcgttgacgc
    10081 tggtctcgct cccggatgcg atgttgaaca ccccgcacac ctctgactcc attgcatgga
    10141 tgagtgccgt cactacgtcc ttcacgtaga caaagtcccg tgtctggcca ccgtcgccgt
    10201 agatcagcgg ggcctttcct tcccggatcc tcgcgatgaa cctggggata accgctgcat
    10261 aatcggaacg cggatcctgc cgcgggccat acacgttgaa gaagcggagg gatactgctt
    10321 gcaggccgta aaggagcgaa aaagccctgc agtaccgctc ccccgcgtac ttggcgaccg
    10381 cgtaggggga gagggggtcg accggcatgt cctccccctt ggggagatcc gggaggtttc
    10441 cgtacacggc ggcagaggag gcgtggacca cccgcttcac gccggaatcc ctggcagcga
    10501 ggagcacgtt cagggtgccg gtgagggtga cctcgtggct ggcaaggggg tgcgcgatgg
    10561 accgcgggac cgataccatc gccgccaggt ggaagacccc gtccacacca tggagatatt
    10621 cgaggagcga cgggaagtcc tggaccgtgt cctggatgaa actgaccctg ggactcggca
    10681 ggagaggggc gatgtgctcc atccttccgg aggagaggtc gtcgatgatc gtcacgtggt
    10741 ggccggcatg cgccaatacc tcgcagagat gcgacccgat gaacccggcg ccgccggtga
    10801 cgacgtagtg caatccttcg ccccttttgt acccctttgg catcaagggg taaaaaaccg
    10861 catgaagcac ccctggtgag cgtgccttgg gcccttcccg aatccttaat gtccacgtgc
    10921 ggcagaacct atcacccatg gtctctctcg ccgagatcca ggccgcccgc tcactgctgg
    10981 aggggaaggt gatccagacc cccctcgtat tctcccccac cttctcggag gtgaccggtg
    11041 cggatgtcta cctgaagctt gagaccatgc agaaggcagg gtcgttcaaa gtccgcgggg
    11101 cagccaaccg gatcttcgga agaaggggcg aaatcggcaa aggcggtgtg atcgcggcgt
    11161 cggccgggaa ccatgcccag ggggtggcag ttgccgcatc gatggcggga gtcgcggcca
    11221 ctatcgtcat gcccgagtgg gtctcccttt caaagcagga ggccaccgtc gggtacgggg
    11281 cccgggtcat cctccgggga agcagcctcg aggagagcat cgcatatgcg aggaaggctg
    11341 ccctggagga ggacatgaca ttcatccatc cctacgacga ccccctggtg atcgccggcc
    11401 aggggacgat cgggctcgag atcctcgagg accttccgga caccgatctc gtggtcgtcc
    11461 cggtcggggg cggcgggctg attgccggga tcgccacggc ggtaaagtcc tcacggcccg
    11521 gcgcccgggt ggtcggggtc caggcggccg catgcccctc cgcggcagag gctttccggg
    11581 agggcgggcc aatcaccgtc gagacgctgc ccaccattgc tgacgggata cgggtgaaac
    11641 ggaccggtga cctgacgtat cccgtgattc gggaccttac ggacggggtg gtccttgtcg
    11701 gggagcaggc gatcatggag gcgatgctcc agctcctcga gcgaaagaaa gtcctctccg
    11761 agggggcagg tgcagccccg ctggccgcgc tcctctcaaa aggggtcgtt gccgcgaagg
    11821 gggagaaggt ggtcctcgtg ataagcgggg ggaacgtgga cacctttctc ctggagcgga
    11881 tcctcaaaaa aggcctctac gcaagcggca ggatggtaca gctttcggtc cccgtcgagg
    11941 agggggccca ctccctcccg gcactcctgc agatcctcgc acgggaagga ggggtaatca
    12001 accgtattga tcaggaacgg agcgcccccg acctccccgt gcaccagatg cgggtgatac
    12061 tggagcttga agtgcggggt cggccccatc tgaaagcgtt gattgtcgcg ctgcatgagg
    12121 ccggcttccc tgcacggccc ttcccggcgg attagtgagg tccacctgca atgacccgac
    12181 atggacagtt gccaccctgt gcacgtcgtg agggggggcc ctgaaggtca gggtgcccga
    12241 cacgaggtca agcctgtcca cgagcccgag ggcaaccacc atcatctcgg cgtcgcagag
    12301 ggcgatcagg aggtccttcc acgactcctc ccggaacgac tcgggcatcc tcccgtgcac
    12361 cccgaggccc gagaggaatc gggcctgcgg gaccgcgtct gcaaagtact cggagaaccg
    12421 ctggaagcgg tagaggcgcc tccagtcgcg cttccgctcc accacctgag ggggcgcggc
    12481 gtattcgatg atccgtatcc tgggatgacg gcggaagttc accagtatcc cctcgagctc
    12541 cggcccgcgt tcaaatccca ccacgaggtc cggctggagg agatcgatca tccggacatg
    12601 gaactcctgg gcggccggct ccaccaccca tcccggcgag tccaccacca ggaacggtgc
    12661 accctcctcc cttgcccgct ctttgagccg aagcgccccg acaagctcct ggaccatgtg
    12721 gccgctcggg gtcactgccc ccacgaaccg gaaaaaggtg gcccgggggg aatcgagggc
    12781 cccgcggtag agggcaaggc cgacagtcgc cggggggccg agggtggact gcccgatgtc
    12841 gcagtcgagg aaacccgaaa gggccgtggc ggcaagctgt gcggtcagcc agcggcagaa
    12901 cgtggtcttc ccccggtccg cagcaccaac tacgtatact gtccccgatc cccgatccct
    12961 gagcgtgcgg agcaccgcct cccattcgcc cgggaccact gtatcagacg ccactacact
    13021 ctccctcttg gtgctctttc ctcataaatg atcagactcg ctccctgaag gaacaccctc
    13081 acgggacttt gtcatgcaga ggatgttctt tcccgccgcg tattcgtacg tcaccatatc
    13141 catcaggctc cggatgaggt gcaccccgag tcccccgatg gggcgctcct ccaccccccc
    13201 cgtgagatcc ggggctttga ccgcgagggg gtcgaacggg tgaccgctgt cggtgatgca
    13261 caccctcgca catgtggaag acaccgtgca ctccacggtc atctcgcctt cgccgggtgc
    13321 atacgcgtac cggatcacgt tggtgcaggc ctcgtccacc gcaaggtcca tctcaaagac
    13381 ggtcttctcg ggaaagccct gctccctcat gaagcctgtg accatgccag tgagcgcctc
    13441 gaggtgctcg agtgtcgcag gaacagtgag gctccgggtc tctgttccgg tcacgatccc
    13501 accttcagca caaggagagt catatcgtcg tactgcggcg cagtgcgggt gtgtgcagcc
    13561 accgcggcct ggatggcggc cagcgcctgg ctggcggtgc gggcccgctc gctcttcgcc
    13621 agttcagcca gccggtcgtc cccgaactgc tgctgtgacg gatcttctgc ctccgtgact
    13681 ccgtcagtat agaggaccag catgtccccg gggcacagcg caactttctg ctcttcgtaa
    13741 gtcgcaccgc tgtctgcccc aagcgcgatc ccgttcattg taagctcgac gagcgacccg
    13801 tctgctcttg cgagcagcgg ggggttgtgc ccggcattca cgcaagtaag cgtctgctcc
    13861 gaagggtcca ggatgcagaa gaagagcgtc acgaacatgc ccgaccctgc ctcgcggcag
    13921 atgagggcat tcgcatcgct caccgcgtcg cgtggccgct ggtgccaggc ggcgcttgca
    13981 cgcatcactg tggaggagag ggccatgaag agcgcggcgg gaactccctt gtcggagaca
    14041 tcggcgatca ccaggcccac tctccccccc ggcgaggcga tcacgtcgta gaagtcccca
    14101 cccacctccc gtgcgggccg gctctcggcg gcaagagtat atccggggag ggcggggaga
    14161 gagggcggga gaaagctctc ctggatctcg cgggcgatct ggagctcagc gttctttcgc
    14221 tgcagctctg cctggaagcg gtcccgctcc ctgcaggtcc tccgctcgtt ctccaggttg
    14281 accgtgatga acgcaaagat gaacatgcct gccgcgttgg cggcgatcat cgggaacgcc
    14341 acatcggaga cgagctcgac cgcctgggag aggggccggc ttatcagcag cacgagcccc
    14401 atgtgcatcc cctcgatgag gacagccccc gagaccgcga gaagcgtccc gggaaaccgc
    14461 cgccggttgg caaggttgat cagccctgca aggaggccgg caagcacagt cgcaagcgag
    14521 caggcgacca tcgtgaaccc gcccatggtc atacggtatc ctgccccgat caaacccgcg
    14581 ccgagcccca cgaccgggcc cccgacaaac ccggccacca tggggccgag gtcgcggatg
    14641 ttgaggggtg ccccgaggat cacgatcccg ctctcggagc cgtaaatgga gatgatccca
    14701 aagaccagga tgaggatggc ctggttcttc caggagaatg tcccctccag cacctctgaa
    14761 tagaaccggg tccgtgtcac caggtaggca aagaccacca ctacgcacat catctggaag
    14821 aggacgatgg acgccgtgac cagttcggag accatggtat cagcggacag gggtgcgaat
    14881 tacaggtgcg gaggtcacgt cgcaggacct gccccaaggg cctcgtcaag gctgccgtga
    14941 atggcaaaga ggcggttgaa cccggcgata tcaaagacct tccggatctc ggggccgagt
    15001 gcggaaagct tcatggttcc cccgcactgt ttcagcctct tcagacctgc aagcagcact
    15061 cgcaggccgg cgctgctgat gtactcgagg cccgagagat cgactaccag atggtgttct
    15121 cccgcgtcga tcaggcggtt gatgtgcgcc tccgcctccg gtgatgtggt ggcatccatc
    15181 ctccctttca ggaacaaaac gggaacaaca ccccggcgct cttcgacaac ctgcagaatc
    15241 gtgctcattg atggatctct ctttatctca tcccatatga gggtagcgag aactgcgttc
    15301 tgccatgaga ggcagcactg tttctcctgg aaaccagggg tttcccgggt ctaaaatacc
    15361 ttcaaataat cgtttttaat cgcttttgaa ttcgaataga ggctcatttg gcataaggaa
    15421 aaatatccac gatcaactat ctcaggaatt cgatgttgct gggaatgacc tgaaaactgg
    15481 ttcggcggat gaaccttctg ggaaaaatcc atccctcttt acatatagca tcatatggtt
    15541 tattggtttt tcggtgctat acctggctgc aggaaaacag cgccctgaaa ccggattatt
    15601 gtaaaaaacg gaaaaattac gcagaaaaag gaacggcagg atcgattacg ccgagaaggg
    15661 gtccgggaag cgacagtgct ccgaagaccc gccaggcact ttgcccccag ataagaagag
    15721 tcgccttttc ccacaagata tggcgccttg tgcgaatgga aataccctat ccctgctcgt
    15781 tcacctgccg gggcttctgt ccccgcttct cttcgtcatc ctttcgcccg gagaggggga
    15841 agaactcctg gtagaccctc tttcgctcct ccaggtcagt gtgcaggtag atctcggtgg
    15901 tcttgatgga agtgtgtccc aggttctcct ggacaacccg caggttcttt gacctgcggt
    15961 agagctcgct tgcatagctg tgccggatct tgtggggagt gatgccgtca ggcgcatact
    16021 tcctgaagat gtgctggaca gcacgcggag agatatggtg gccctgctgg ccgacgaaga
    16081 gaggcccctc gatcctcttt tcgatgaacg catccatctc gccaagcgta tcagggtcca
    16141 cgaacacgat ccttatcttg ccacctttcc cacgcaccct gatggtcccg tcctcgaagt
    16201 cgatgtcctc gatgttcata tcgcagagtt ctgagacccg gacaccggtc gcgtagatga
    16261 cccgcacgat cagccggtcc ctctggtcct cgacggagtc aatcagacgg agcacctggc
    16321 tgtgcttcag gtaccggagc tcctggtcct tgatcttggg ccgctcgacc gcgaccatcg
    16381 ggttggccga gaccgctccc tgggagtaga ggaaccggta aaaggaactt aaggtggaga
    16441 ttatccggtg gagggtcttt ggcttgtagg tccgcatcga ggtcatgaat gagaggtagt
    16501 cttcaaggag cacgggctcg acgtcgacgc tggtctcaag cctggccttc gagaagtcct
    16561 cccagtagac catcagcttc tggggatcga tatttctccg gagccacacg tagtaggcaa
    16621 agtgcttcac cacctgctcg tagctctgga tggtccgggg ggagtaattg cgcattcgca
    16681 ggtagtggcg gtagcggttg agccattccg agaaaaatcc cccctgcatg ctctattctt
    16741 tttttcctcg aattataaac tttttgcgca gaatctccat ttcgcgacaa aaccagatgt
    16801 ccgcccctgg cggaccatgg ggagctccca tgcgggtgca gccattttgg atcatcctct
    16861 ttggagaagg ggttgaacgc gccggatctt ggtcccggac aggattttta aaaaactaaa
    16921 aattccggaa cttcaattta catgaaattg gcgtatgtat cgattatact attcgtcctc
    16981 tttttttttt ggcgaatgtg cgaaccttgt gcttctgtga cgcgtatacg agatcttcca
    17041 ggttcatgcc cgggtcatgt gaccgatgaa ggaaatgttc attcctcaat tgcaagggac
    17101 tatttcgagg ggcccgggta tcggaccccc tcctcacagg cagggtgatt accccatgag
    17161 tccatgaatt cccctgcttt taaaatgacg gcatccgtcg gacagcgatg cattgggaca
    17221 agtaattaaa acggattatc cctcctttac cttaaaagag cacaaacttt cattgggagc
    17281 ccgaggcact gctcaagtct ctcgcacgat tttattcccg ctcgatgata ttctctattt
    17341 tgcattttcc cacctctcat actataaaag acggcgaatg gctaaatgga ctgaagggaa
    17401 gtccgtttcg ggaagatagc cggagacggc ggtgttgaca ctctcctgcc gatgtttata
    17461 ctcatggaga atcgcaggga ggcaaagcgt aagtcccgaa gtacagttgg atgattcaca
    17521 tgcgctggtt caccagatac gcaaaatggt cgtgattttg gagatgaaac ggagaatgcg
    17581 aagagattcg cggttccaac ctccccttcc acttccattt cggcaggtag ccggtggaat
    17641 cgctcacagg atcagcgcaa tatacagtgg ggagagactg aatcgggcga attaccttca
    17701 gacctgtcca gtcactgaac cgccgccctc ttgcgtgact tgttttatag ttcgtcgtta
    17761 tctggtgacg acaggattgg cgaactgaat acatttgtgg gggctgtccg cccccacacc
    17821 ccaaatgcaa tgcaccccgc cgcccttgaa cataccgcgg ggcttccgcc ctcgacccgg
    17881 ggcgacctgc ggtcaccttc agatttaggg ccattcctga gtaatctggg atgagttctg
    17941 tgaaaaagca cggcggtagt tggagtgcct gatgtgctac acagataata aaattgattc
    18001 tatattccat acaaaacagc gaggaggctt ttttattgtc atgaactcac cctcgtttta
    18061 ttggatggca tgattggcga tttgaaaatc ctctcggacc ccaatgagta gagcctgcga
    18121 agagagggct ggggcagcca ggatcacttg cggatgcgcg gtgtaacaat tcacccacac
    18181 tttttctgcc aggactatta tagccgtgtt tcccgatgat aacccgagat ttgagggaaa
    18241 aattgttggt gacagtgacc tgttacccca atccgttccc agggagatgc atcccgactt
    18301 tttttattat ctggattgtc ctccttcgtg cgatgcatat agtgcaggag gtgggagctc
    18361 taggtttttc tgcaataatt ggcaataccc gtgtgtgccg gcagccagtc ccgggggaag
    18421 gctcgtgaca tggggagaga gaaatgcctc gtttggtccc aacctccatg cttcatgaat
    18481 tgttcatttc attttctgcg gacgttcatc catggagaaa agataatttc cctttttgcc
    18541 gtgatctttg aacgaggaat cctttcattc aatcctccca cccattcggg atatggcggt
    18601 ctctctcacg aaatcacggg gatatccctg cttttactca aaatcacctc ctttcatcgt
    18661 gatacacacc agccggatct cagatccacg gcgcagttgt tcgccctcac accccatggt
    18721 gcagcacaca tcgtttgccc atggaattgc cgcgaaaggg tccatctttc gaaaccggag
    18781 gcagcacttc ggccgttaat ggagccttca atgaggcacg attcaagcga attaccagga
    18841 accgtctcct ggagacggct gcggtcgaga tgaatggata tgtacgcaac tcacaccctc
    18901 gatccagtct cccatcgaaa aaaagcgaaa ggatacggga agaaaaacca gactggacct
    18961 catctcatgt atccccgggt ttttcattat gatgggagaa agggagaatg aattgtgccc
    19021 tccattccgt aacatccggg ttggaaattt cactttcacc ctgcccggcg gatatttttg
    19081 agcggtgcct ccaacatagg aaaatgtctg tatcaatgcc accttgagga tacagacatg
    19141 ggcagaaacg cccatggaaa aaggtactct gccaacagaa acctggagtt acacgggatc
    19201 ccggtacccc ctttcttacg ggatccccac ttttctcctc tgcagattac cattcgaagg
    19261 tttctaaaaa atcggttata aatgactcat tttgaatgaa agccatgaat ctctgaaatt
    19321 tctatccagc cggaacatcg cttccaatta tccatacacc gcatgttgct atatgttacc
    19381 ttggttgcat tgggctgcca ataacgagaa aagaaggcgt ttggaagaga tacgatacta
    19441 ccggattgcc aacaggcagg acaacgggtg ggaggggatt tgagaacaga aggaatagta
    19501 ttcaccgaaa cagccccggt tcaaacgaga cggtccaatc ggtgatcctc gggcaacgaa
    19561 acccaaagag cgcccttgat tacccagata cctatttaaa gggaacagaa accatatatg
    19621 cataaaaaca gcccccccct gctggcatta catcccaatg atagaccatg attacagtcc
    19681 tgttcgtgga tgatgagccg gccctcctgg atgtctcaag gttatacctg gagaagactg
    19741 gggatctcaa ggtcgacacc tgtttctcca cagagcaggc aatagagatg ctcaaggaac
    19801 gatcttatga cgtgatcgtc tccgactacg agatgccagg gatcgatggc atagagtttt
    19861 tgaaactgtt gagaaacctc ggagacagca cgccgttcat catcttcaca gggaggggaa
    19921 gggagcatgt cgtcatcgag gccttgaaca gcggggccga tttctacctc cagaagggag
    19981 gagaccccaa gtcacagttc gcagaactca ttcacaagat caggctcgcg gtagagaggc
    20041 agagaatcga gagatcgctt caaatcaccc gcgaggctgt ggagaaggcg tcagtcagga
    20101 tcttctggct tgattcgcgc ggcaggttca tctatgcaaa cgaggcaacc tgcagtgcgc
    20161 tcggatacac acacgaggaa ctcatgcgcc tgaccatcgg ggacatcgat cccttttttt
    20221 cgagggacga atggaaccgg ttcacagagc gattgcgttc gcaccggtcg atggtcctgc
    20281 aggcatttca tacgagaaag gaccgcatga agatcagcgt ggagatggcg ttcaatatcg
    20341 gagagatcga tgagcgggga atcatatttg cctactcctg gaatgcgagg gagagaacag
    20401 ccctcgatgt actggcagca caggcggatg cccgctgcca caggattgca gaagcctttc
    20461 cccatcttta cttcgaagtc gaggccgatg gaagactccg gttctggaac aatgctgcat
    20521 cggcattcac cgggcaggag tataaagacc gcacacacat ggggggaaca ttccttgaca
    20581 ttgtcatgaa agggcagcag gcgcacgttg ccaataccat ctccagggtg ggggaaggtc
    20641 accaggacga ggtcatccag gtcccactca ccggacacgg ggacatgcga aggaacgtca
    20701 tcttccgggc acgtggattc gagggagagc acgggggcca ttttgtccag ttgctggaga
    20761 taccccccga gtcctgaatg aaacctccat catcgggcgg ctcctccacg cccgttcttt
    20821 ggtgaggtct ggcacccacc tggaacctgg acatcgtgga ggagcgtcga atcaggcaaa
    20881 aaattggggg gaatgttgaa aagagagagc catgcatgtt tttcgcgttt acgcttcacc
    20941 aaacgatccg cactgcctgc tgatcttttc ctgaaataat tcattatcca cctttttttt
    21001 aaaaaaattg cgtgatattg cactttaaat aaaaacatcc gcagcactat aataaactat
    21061 ataatgcgat atgataagaa attgaaagag tatacgggcg caggaagcga tcggggctca
    21121 aatgcgcaag ggaggaccca aatcgtgacg caatatggac aggaggatgt gaacaagtac
    21181 cggaaattca agaaggtgga tggtgcgacc taccggaagg tgaaccagtt cctgagaaag
    21241 cggacctacg tcaccgccag ggaatgggca atcgcacgtc tttgcactga cttccggaca
    21301 cggagcggcg cggagatgac cttcatcggg cagcacctcc cggaactggt accgttcatg
    21361 caggaatgct atacccccca ggcagtaaac caggcccgca attcgttcaa gaagaaggtc
    21421 tacaagtcgg gagccacgtt cttctacggg gcgatgtgcg ggttcttcac gagcgaagag
    21481 cttgatgacc tcctcttcga agcaagcgaa gtagcccgtt ttctcctgga ggtagaacgg
    21541 acgtcacttg atattgacga ggagatcgag atcgaggacc ggatcacgga cgtgatgcga
    21601 agcgtggggg aggctgctgc acagatcctt cgggccagga tgcacaatga ggaccagaca
    21661 ggaggagagc ccgaagttgc cgggaaccct cccgaccagg agcaggtatg agggtaatcc
    21721 acattgccgg agtctcggga tcaggcaaga ccaccttcat ccggtcgctc atccccctcc
    21781 ttgagcagaa gggccccacc gccgttgcaa agcacctggg gcaccattgc tactccctcg
    21841 agaaggggaa ggataccacg atattcatgg aagagggggc acatgtctcc gcaggggtag
    21901 acatagagaa gaccgtggtg gtggcacgcg ggctctcttt tggagatatc ctcccgctgc
    21961 tgtcatctat tggtacagaa tatttgctgg ttgaggggtg gaagactcag ccactcccga
    22021 aggtggccat cggtgacctg ccgggcgcag cagaagtgat tctttccaac ccaaccgccg
    22081 cggaggtcat tgcatccctg gatctcttcc cccggtatta ctctctggaa gggcttgcac
    22141 aaaaagtgca ggaaggatgc acgggggggg gcgtggtgct gacaggccga tacccggtgc
    22201 cggatcatgc aggtaacccg gagtcaagaa gagagtttta tctccggttt tcacccaccc
    22261 tacatgagat atcacgggtt gcggaatccc gccccggggg agtacggacc atggtgcacc
    22321 tgcaccaggg gctcctcttc ggcggggagg atgggatcct cgtggcagtg ggcgcaccaa
    22381 ctcccgcaga tgccctggat gcgttctcat cctgccatcg gcacctgctc tccgcacttg
    22441 gaaccggcag cccgcaccag ggatgaacat ggaaggacag aaactcgaga agaagctctt
    22501 cggaaccaac ggcgtccgtg gagtggcagg aagagagatg acccccgcgc ttgcgctctc
    22561 gatcggagca gcactcggca caatgcgcca gggcaccatc gccgtgggca gggacacccg
    22621 gacatcgggg ccggcgcttg catgcgccct gaaagcaggg ctcctctcgg cagggtgtga
    22681 cgtggtggat ctcggggtcc tgcctacccc tgcgctccag tatcttgtca gggaccactt
    22741 cgacgcagga tcagtggtga ccgcatcgca caaccccccc gagtacaacg gcattaaggt
    22801 cgttgagccg gacgggacag agatgggcga cgagcagagc atcctcctcg aagaccatat
    22861 cttcagggga actgcctacc aggccgcatg ggaccacgtg ggccgcgaac acgcggcgcc
    22921 gtacatggtt gaggactacg tgcgggcagt cgcaggttcc ttcccaaccg ggaccggcgg
    22981 cgggacactg gtggcagcgg atcccggatg cggggcggcg gcctggacca cgcccgagat
    23041 cctcacccgg atgggatgcc gcgtcttctc tatcaacagc cagatggacg ggaccttccc
    23101 gggccggctg cccgaacctt cccccgaagg gctcgccccg ctcgccgacc tcgtgaaggc
    23161 aacgggggct cgcttcgggg tggcccacga cggggacgcc gaccgggcgg tctttatcga
    23221 cgacaagggg aggttcatcg aggagaaccg gcagttcggg ctgatcgctg acttcatctg
    23281 ccggaatcgc cgcggggttg tggtcacgcc ggtcagcacg tcaaggctga tcgaggtgat
    23341 cgcacatcgc catggatgca gcgtggtcta caccgcggta ggcagcatct atgtggccag
    23401 gaccatgata gggctctcga aacagggcac tgcagtcgca ttcgggggcg aagggaacgg
    23461 cggactcata ttccccgcgc accagctctg cagggacggg gggatgaccg cagcgaccat
    23521 ggttgccctt cttgcagaca cgtcgcaacc gctctccacc ctcgtggatg cgctcccacc
    23581 gttcttcatg atgaaagaga agctgcagga gaaggatgcc ccggggctta tcaggcgcct
    23641 gaaggatgca tacagaggag agtcgcttga cctcaccgac gggatccgca tcaaccgtga
    23701 agactcgtgg gcacttgtcc ggccgtcggg aacagagcca ctcgtgcggg tgatggtcga
    23761 gtcgacctca aagacagggg cgaggcagtt ccacgaggag cttatggccc atatcaggca
    23821 gtaaaccaca gccaggcatc ccattttccc ctctttccaa tgaaacgcac atgataccga
    23881 tcggtcacat gcccccctga tgaaaaccgc ggatgcggtt ttcataccaa aatgcctgcg
    23941 gcattttcat gcgttatgct cgactgcaag gcggtcatgg gtggttttta tgatgcaggg
    24001 cttccttgga cgatctccct ggcaatggtc gcgaaccggg catctcgcca aacatgggcc
    24061 actatcctta tgcaagctca ataattcata gaaatgtcat atttttggga aaaaacgctt
    24121 ttttactcat tttttagttg atggcgtgta cctttataca cttcatgttg cgatatcata
    24181 acaggcgaac gctggaacgg gaatactgat atgccacgcc gtcgacaaat ccaatcgtct
    24241 gaccacaaaa gggtgaatga agggagaccg cattgaatgt gaggcaccat gaagacagtg
    24301 gagatacgca tctccggcag ggtccagaag gtagggatgc gtaactgcgt ccggcatatt
    24361 gccgggaaac tcagcatccg gggagacgtg atgaaccagg cggacggaac cgtcaggatc
    24421 agggccaccg gggaccccat catccttgat aagttcgtct ccatgctgta cagctgcccg
    24481 cgggccctca tcagggacat cgagatcctc gatatccagt ttgcccagtt tccggacttt
    24541 gcagtagtca ggaccgcaga agagacccgt taggataccg tctcaagtga tgacgaggta
    24601 atcgttcttt ttgatcaatg caccctgaag tgccatcacc tgctcgtcga tgacttcctg
    24661 gcggattgtt tcgtggagag ccttcgtgag caccacgagc agatcgtcgg agagaatgca
    24721 gcgggaagga ccgcactcgt attcctcctc ggggattccc tccctcgcaa tggccgagac
    24781 catcccgtcg accatcgcgg gcttgaacgg gtcgattata tcatagacga ggccgccctt
    24841 tcccttgtga aggaacccta agtcagggtc aaggtgtgcg ccgatcgcgg ccacacaggc
    24901 atttccaaag agaaggccgt agccgaagga gagcatggta ttcacgacat ccacatgggg
    24961 ccgggcagtc cgccgcctga accctgtctc ctgaggaatg gtgcgggcaa ggatctcgta
    25021 atacatatcc gatgcgaggc ggtgaaccct gcgcaactcc tccactctga cgaggaactc
    25081 gagctcggcg taactcttct ccaggatctc gagctctccc tcgtaaaaga gtggttcgtc
    25141 gcgtgcctcc tggagcccga caagcgcctg gatccgtgaa tggatactgc ctttcgctac
    25201 tgcaagggca aacgaatggg agtatgcctt tgcctgcgct tccctgacca cttcgtcata
    25261 acggaaacca aatggttttg cgattcccag cggttctcca tctgcttcga agaaagatat
    25321 gcaggacccc gatgcgagca gccgtgcaac cacggaggta tgaatgtgat ggccgcccat
    25381 caccaggaga tgccggaccc tggagagtgg caactcctca gtatgcccgt tcttctgcac
    25441 ggtcagggtt cggggtgttg ccttcaggtg ggccccgaat ccggacacca ggtaccaggg
    25501 gaccggctcg atgacgctcc actccatatt tttcttaaac gaggggaagg tttccgatat
    25561 actcatttcc cggattcttg tacttcaatg catcgttatc ctggagaatc ctccttttat
    25621 tggcaggata cccggactat cgacgggagc catgctatga cttgacaatg tttttcatcc
    25681 gccggcacaa gactctatga gaggccgcgt tggatctcgc acgtgcaaca attccctgca
    25741 cggaaagcag acgaaaaacg ggctgaaccg gtggcagaat gacgagaatg acaggagccc
    25801 gagaggacct ggggacaagg gaccatgttc tgggatgaga agaccgagac gctcccaaaa
    25861 agagacctcg aggagttaca actcacgcgt ctcaagaaga ccatcgcgca ggtccagaac
    25921 gtggagttct atcggaagaa actgaaggaa gccggggtgc ggccggatga tatccggact
    25981 ctcggagaca tagagaagat cccttttaca agaaagcagg acctccgcga cggctacccg
    26041 ttcgggttct tagccgttcc cctgcagcag gtggtccgca ttcataccac ctcggggact
    26101 acgggaaaac cgaccgtggt gggatataca aggagcgacc tcgagacctg ggcgggcctg
    26161 attgccagga acatgacgat ggtggggctg acagaccggg acatctttca gaacatggtc
    26221 aactatggga tgtttacggg cggtctcggg ttccattacg gtgccgagaa agtaggaatg
    26281 acagtgatcc cgagcgcgac agggaatacc aggcggcaga tcgagatgat ccaggacttc
    26341 ggggtcacgg ccattcactg cacccccagt tacgcgatgc accttgcaga ggtcgccgag
    26401 gaggtgggag cacacctgga ctccctttcc accgggctct tcggtgccga accatggtcc
    26461 gattctatgc gggcggaact cgagaagaag cttgaattga ccgcatttga ctcctacggc
    26521 ctctccgagc tcttcgggcc gggtgtggcc ttcgagtgcc cggaacggga cgggctgcac
    26581 atctggcacg actgctacct tgtcgagatc atcgatcccg tgaccgggga aacgcttggc
    26641 gacggcgagc ggggcgaact ggtagtcact cctctcgtca aggaggcgat gccgctcctc
    26701 cgctaccgca cgggagacat caccatgcgc ttcgaggaag actgcctctg cgggcgggca
    26761 aagaagatcg cccgcataac agggagaacc gacgacatgc tggtcatacg aggaatcaac
    26821 gtgtttccgt cccagatcga gcacgtgctg ctgcggatac ccgaggtcgg aaaccagttc
    26881 atggtctatg tcgaccgcat caaccacctg gacgagatga cgatcgacgt ggagatcaac
    26941 cgtgactact tctcgggcga actgcaggat ctcgcccgca tccagaagaa ggtagtcaag
    27001 gaactgaggg acacactgga gctccggacc accgtgaagc tggtggagcc gggttcactt
    27061 ccgaggttcg aagggaaggc gaagcgcgtg atcgaccgaa ggggtgagat gtgatgaggg
    27121 catgggaccc ccgcatcgag gagatgccaa aagccgatct cgaggtgatg cagtacaggc
    27181 tcctaaagac gctcgtctac cggctctaca gtttcagccc gttctaccac cggcggatgg
    27241 aagagaacca tgtccatccc gatgacatcc ggtcacttgg ggacatcagg aaactgccgt
    27301 tcatgtacaa gcgggatctc cgggacaact acccggacgg gctctttgtc tctacgcagg
    27361 aggacctggt ccgctaccac gtctcttcgg ggacgaccgg gaagcccact gtcgttgggt
    27421 ataccgagag ggaccttgag aactggacgg cctcgcttgc gagggggctt gtctcctgcg
    27481 gcctcgggag gggggatgtc atacaggtga gctatggcta cggcctcttt accggcgggc
    27541 ttggcctgca ctacggggct gagcggatcg gggcaaccgt gcttccgacg agcgtgggga
    27601 ataccgagcg ccagatcgag ctgatgcagg atctcaaggc caccgcgatc gcctgcaccc
    27661 cgtcgtatct cctgcacatg ggagaggtcg cggagaagat gggagtcagc atcaaggatg
    27721 atacccggtt gaaggtcggg atcctcggcg cagagccatg gtctgagcag atgaggcagc
    27781 ggatccagga ctggctcggc atccgggcct atgatatata cggcaccagc gaactttcgg
    27841 gcccgatgtt ctgcgagtgc agtgagcaga aaggtttcca cgtctggggg gaccttgcat
    27901 atgtcgagat cctggatcct gacacactgg agccgcttcc ggccggagaa cggggggaga
    27961 tggtgatcac catgcttcag aaggaagcgc tccccatggt gcgataccgg atcggggaca
    28021 tcagcgccat cgatgactcg gtctgcgcgt gcgggcggac ccacccgaga gtacagcgga
    28081 ttacggggag ggtcgacgac atgctgatca tccgcgggat caacgttttc ccctcgcaga
    28141 tcgagtatgc gttgatgtca attccggagg tggggcagca cttccagatc atcgtggaca
    28201 ggaaaggcgc gcttgacgag atgctggtac aggtcgaggt gaagagagaa gcgttctcgg
    28261 acaagatcac tgacctgatg agcatcagga agaaggttga gcacaagctt cggaacatcc
    28321 tgaacgtggc ggtgaacgtc gaactggtcg aaccgggatc cctccccagg ttcgagggaa
    28381 aggcaaagcg cgtgatcgac aggaggaaga tctgaaatga aagagagcag gttcgtaatc
    28441 aagcagatat ccatattctc cgagaacaag cccggccggc tcgcagccat cgcccgggca
    28501 cttcaggaag agaagatcaa catcctcgcc ttctctatcg ccgaggcgga cggcttcggg
    28561 gtggtgcggg cgctggtgga caagccgaag aaagcccatg aaaaactggg agcgcttggg
    28621 ttcaacgtgg cctttaccga tgtgattgcc atccggatga aggacgagcc cggcggcctc
    28681 tacgagaccg cgcggatcct gggtgagggg cacatcaaca tcgagtacgc ctatgcctac
    28741 tcgggaaaag atgccgcggt gctcatcctc agggtcgaca acgtcgaggc ggcgatctct
    28801 gcgatccaga agggtggggg gacgctcctc gagagctccc tcttccagta aaccctccta
    28861 cttcttctcc caccgtatca ggtccgagac acgcgagagg gtgctccccc tccgaatctc
    28921 ctccctgacc cgttcctcgt tcttttttac ttccatcgcc cggcgggcaa tctccatcgc
    28981 ccgctcccgc gggattacca ccactccgct ctcgtcccct gccacccagt ctccggggcg
    29041 gaccacctgg cccccgcacc tgatctcggc gttgatctcc ccaaaaccct tcggttcacc
    29101 tgcattcggg accacggcac gggagaagag cggaaaccgc atcttccgga tatcatcaac
    29161 gtcccgtgcg gccccatcga tcaccacccc tgcaaggccc ctctccatcg cgctcatggt
    29221 ggcgagctct ccccagcacg ccacgtgggt tacgccctgg ttgtcgatca ccagcacgtc
    29281 gccctggccg gcaacgtcga tagcctcgac gggcttggcc cagtccccgg cgaatccctg
    29341 gaccgtcacg gcttttcccg ccattttcac atccctgcac agcggagata tccccgccat
    29401 cgcaccttta cggtgcatgg catcggagat attgggggtg gagaccacac gcaggatgtc
    29461 ccggatcgcc tggtccgggg atacctgctc ctcgcggggg agggtgggac tgtcgattgc
    29521 ttccctgatc aatctcgcag actccgtcac cgttgcggac cgggtaatcg ctcctcccac
    29581 gatcaccacg tccgcacccc cgatgaccgc ctggggggcc gtggtggcgt caagacctcc
    29641 cgcagccgca atcgggacat tcaccgaggc tcggagcgat gcaagcatgg agagggagct
    29701 cttccctatc atctgctggt cgatgcctac atgggcgttg atgatgtgca cgcccatctg
    29761 ttcaagttcc ctcgcacgca agatcgggtc ccggacattg atcaggtcgg ccatcagccg
    29821 gaccccatac aggtcggaag cacggactgc ctccgcgatc accgagtcgt ccgcatcggc
    29881 aaggatgcag actacagagg ctcccgcttt tgccgccatc tccacttcga gcgcccccgt
    29941 gtcggcaacc ttcatgtcag ccacgacctc gcggccggga aagagggcgc ggatcctcct
    30001 caccgcctcc atcccttcgc tcttgatgag cggagtccct gcctcgatcc agtcggcccc
    30061 tccctctacc gcctcccggg caatctggac ggcccggtcg agctctgtga tgtccagggc
    30121 tacctggagg atcgtccgtt tcatgtgaga gatgtggtat ggcatgggct aaattgttgc
    30181 tgaccggggg ctggcgcccc tcgaccccca ggcgacctgg cggtcaccga atggtgttcc
    30241 taaaaaaact gactgaacat atccctgcgt ttttcatcat ctttactcag gctggcgccc
    30301 ctcgaccccc gggcgacctg gcggtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30421 nnnnngaaca tatccctgcg ttttcatcat ctttactcag gctggcgccc ctcgaccccc
    30481 aggcgacctg gcggtcgccg aatggtgttc ctaaaaaaaa ctgactgaac atatccctgc
    30541 gttttcatca tctttactca ggctggcgcc cctcgacccc cgggcgacct gaaggtcgcc
    30601 gcagtccaat ggtgttccaa aaaaaacact gactgaacaa atccctgcat ttttcatcat
    30661 ctttactcgg gcttcgcctt tcgaaaccaa ggcgacatag cgatcgccgc agtgcgagaa
    30721 ggaaaaatga aatcccggag cagaaaggac ccaggaaaaa cagagtgatc cggggccatt
    30781 cctcgcacaa ccccgcagtg ggggctccat cccccaactg ctccctcgca taggcccaaa
    30841 tcgcatttca gtgacgcgca cgcgtgtgcg gaaagctcct ggcgcatcac cgggggtgaa
    30901 gtgcggtcgg gtttgcccga tgaaaagggt attcctggaa cacccgcctg cacacccccc
    30961 gcagcaatag gcattgtgcc tattgctgct ccttgtactt atcggagaac gtgatgtcgt
    31021 ccaggtcctt cttcttctgg atcgcaatct cggccgggaa ccccgcaggg accagcgaga
    31081 cgagcgtgta gttgcccggc acatcgagga gcttcctgac tgcctcggca tactctttct
    31141 tctcccccgc aacccagcag ctgccgacgc cataagactg gaggccgagg atgagagtct
    31201 cggtcgccgc acagcagtct tcaagatagt acttggcctt cttctcacca aacacggcaa
    31261 tgcagatcgc agcatcggcg atgaacttcc cgtggtcggt gaggtcggcg atctttttga
    31321 ggaggtccct gtcgcggacg acgccgaaga gccagggctg caggttcatc gcggtcgggg
    31381 catgcctggc acattcaagg acatcccgga tcgtggtctc gtccaccggg tcaggtttgt
    31441 acttgcgcac gctgtgccgt gacttgataa tggtggttcc gacgttcatg gagattccat
    31501 cggtgcatga gaaaaaaaag gtgtccttcc cgcactgacc gggaggacga ttgtcagggc
    31561 atccttccct ttcgcaccaa aagggccagg gctgccagga acgccagcac cgcggcgaaa
    31621 cccagaaatc ctggtgccgg agttggggtg ggcgtgggag gcaggggcgt tccggtctcc
    31681 tggaccgttg gcgttgcggt agtagcggtc tcaatcgggc tcgccggagg agacccggca
    31741 ggcgtgggag tgcgggtcgc ggtcggcaca ctcgtcaccg gcagtggagt cgtccctgta
    31801 atcagcgggt tgactctctg gacgaggaat gtgaccttct cgctgatggc ctttcccgcg
    31861 gccgggtaat tgtcggacat gtcgtttgca tcgcaccgta cataaacaga atactccccg
    31921 atgggatact cggcactccc ggtcgcccac accgggccgg tctcaaacgg cgacgaggag
    31981 acgaccaccc cctcgagaga atacccgccg agggatgaga atgtcagcga gtccgggccg
    32041 ttcagcctga tggacagcgg cgcgccggca gttcccggcc ggttcgccat gacccagagg
    32101 ttcgtatcga cccggaatcc tgcggtgtcc cccttgggaa tccaggtcgc cgacgggctg
    32161 accacgaacc cggaagagta gtccaccacc cgtatcccga ggctcggctc ttccaggtag
    32221 aatgcgacag tattctcggg gaggaggaac cacgggccgg tcttccctcc aaacgtaatt
    32281 ggagagacat agaacgcgga cgggtcaccg accgtcacct gggccgcggg ggcgtgcgag
    32341 acctgcccgc cagtaccata ccaggcaagc gaggaccccg cggtggcacc ggtcagggtg
    32401 atgtcaaggt cctcctctcc gacaaagacc gtccccccct ggggtatggt attctctgcc
    32461 gcggagaagg gggcgaccag gataaggagg actgccaggg caaggatcgc tttgtaatta
    32521 tagtgaccct cattcgatcg tttcatagac aggagagcgc aaacccgcct cataaacctt
    32581 ctcctgcgat gaagaaggag gttttttaag gacttccttt caggagaaga tcccggtgat
    32641 ggcattccac atgttggaga acccctgggt gatcagcacc ccgggatcga ttcccagcac
    32701 cgggaatatg aacataaagt agaggacagt cccgagcgtg ctccccaggt tggcaagcgc
    32761 ggcaacgagg acgacccgga agagcggcac tttcatcatc tccgagaaac tctcggcctc
    32821 gaagatccgg cggaagtcag caggtgccgg ttttcgtatc ttcgcctcca cgatggccga
    32881 gaaccagccc gctgcaatca gtgggttcag cgccgtgaac caggagaccc caaatcccgt
    32941 gagtgcggag agggggtggc ccctggcggc aagggtaaat gccgcggtca ggatgccgtg
    33001 aaccagcacc cagtacacga gtgccatccc cagcacctcg aagcccacgc ccgagaacgc
    33061 gagcgccagg aggaggagga caaagaggac cgtcaccgcc actccgaaga tcaggcccca
    33121 gggccgcgac ttcacctccg cagtcagctg ctgtatcggc gggagagtcc cgggtgcatc
    33181 gagatacttc cggacaccct ccacatgccc cgccccgagt atggcaagga cccgcgcatg
    33241 ccgcgttgtg aggcctgcga gattgtgggc aaggtaggca tccctctcat cgatgagtgc
    33301 gcgtgcgccg ttcggggaga acttccggaa ctcctccatg gcggcagtga gcatgtcctg
    33361 ctccttcagc tgctcgacgt ctatctcctc gccatcgatg ctggcgaccg agaccgcgag
    33421 ggcatagagc atcttcagct tctcgaagaa cgagagcgac ctccagaacc tgcgaagggt
    33481 gatgttgatg tcccggtcaa cgaggccgat ggcgatcccc cgcgactctg cctcctcgat
    33541 ggctgccttc atctcggcac ccggctccac gcccaggtca agcccgatgc gtcgctggag
    33601 gtatgcgagg atccactgga cgaggatctg ggtgaagttc tttgcttcca ggatatcctg
    33661 gaccgacggg tcttttgcct gttttcgtat caccgcatac cgggcggggt caagttccac
    33721 tgccaccacg tcgggagaga actcctcgat ggctgcccgg acctcctcaa cgctctttgc
    33781 cgagacatgg gccgtgccca cgatccggag ttcccctgtc atactccctt cactccttca
    33841 ccatcaacga taaggcgccc ctccccttgc ggaagcatcg agaacgcccg ccgtatcctc
    33901 tcctcctgtt cctgttctgc agctctccgc gcaagcaggt cgcgggccag ggcgcgggcc
    33961 tcttccggac caacagggct cccaagcccg gggcagggaa acgtgagcaa cgtatcgccg
    34021 tcaagcatga agggataggt gcggcatatc cagggcctcg tggcataggc ccggcacgat
    34081 cccccttcgt agaacatgca gtcgccgctg atacgccgta tcgcccatcc gaacgtgatc
    34141 ctgcggccgc ccgaaatctc cacggactcg gggtagggct cggcgacctc tgcggcatcg
    34201 aggcggcagt gtgcggatat ccggcggacc tctgcaggag ataccatcac caggttgtcg
    34261 tccccgttcc ccgcgcggca gcactcgccg cacctggtgc aggagaaccc tatctcctgg
    34321 aggcgggcag ccagggtctc ctcgcacatg gatgattcgt tctccttcat gctccctgtt
    34381 ccctgcagat tgcgtcaaaa ttctcataga ccctgagacc atcgcaagta tggctcactt
    34441 cagggtgcca ctggaggcca tacaagtgca gatctggcct tgcgatcgcc tcatgcgggc
    34501 agatggcaga cctcgcaagc agccggaaac caggaggtgc ctccgcaacc tcgtccgcgt
    34561 gggacgccca cacgcttatc gtctccgggt atccggagag gatctccccc gcgtccacca
    34621 cttccacctg caccgggccg tatccgccag acctcccggg ctgcactctt cccccgaatt
    34681 ccatggcgat gatatggagg cccaggcata tcccgagcac agggagaccc aggtgcaggt
    34741 aacggcccgc aagcccgctc cttcccatgt ccggcccgcc gccaagcacg atccctctgc
    34801 aggcacctgc cagctcttca gggggtgtcg tgtttggaac catcctggca tcgatatcga
    34861 ggtcccggaa catgcgcagg atgaggtgat tgaactgccc gtggttgttg accacgaata
    34921 tggggagcat gatcttcact agaatggcgt gtgaaagttc aaaatgtctc cggcatgtac
    34981 attttgcgtc catgccatcc tttggaggtc atgagttctc acttcatgtc ccggccatgc
    35041 acccggttgc ggcagatcag acatctgtta cctccggttc cctttcagtg gtaggatgcg
    35101 accgtctcga gtatctttgc ccggattgcc gatggggaat tgccgaggag ataggccatc
    35161 agcatggccc cgcctgcacc cattccctcc ttgacttcgc ctatgcagta ccgcgcgagg
    35221 ccggaatgcc cgagatcccc gaatccgggg tccacatata ctacctccac cccgatcctc
    35281 tccgcgatct cgcaaaaatt cgctgatggg tcgtcccgga catacgcggt ggttgcaaca
    35341 agggggaggg ggatttccat tgccttcatc accgcggcca cggcaagcat ctgggtacct
    35401 cccgccagca caatgcggcg ggtgcaggtg ctccccatcc ctgcgcagac agggatcatc
    35461 gggtctccgc agcacctgag aatgtccatg ggagattcca cgccctcagc cctgatccgg
    35521 ccggcaaccg cgttccatac ctcctctttc atcttcacag ggttctcgat gaaacttgag
    35581 ctgacccttg caggataccc gagggcacga agcacgcaga gcgcggtggt cgtcccaccc
    35641 ggaacacact cccccacaat aaggaggtcg cacatccggg agaggaactc cccgagcgac
    35701 tcgccggact ggaacagagc ctctgcacgc ggaaccgcgt ccatgaaccg cgggtcgttt
    35761 cctgcctccc catagacgtc catgcatggg actgtaggaa ggtgggagag ccccgcattg
    35821 ataaacaggg cgcgtgtccc gcagagctcc atgatggagc gggtgatgga tgcaggagtg
    35881 ggacagcccg taggcgtatc gggccttgcc ggcacgctgg taattgcccc cttggcgatc
    35941 agttccgcat cgagtaccgg ggtgagcagc gtcttctccg gggtcgggcc ggccccggag
    36001 atgccgggga ccacggaaag catcgtgttc gcgaggatgc tgcagaacag gggattttct
    36061 gcagcaatgc ctggatcttt tgagaggaat gccatggaca cactcttctt caacaatcgg
    36121 ggccgcatgt ccatcaacct agcgccccgt ggcgagtggc aaccctgata accccgcaga
    36181 agagaccagt gtgagaggaa ggatacctgt catgtttgag attggtgaac ggttgcgcca
    36241 gaaaggggcc gggatggatg agatagtcga tgcggcgatg gagctctacg tcccgcacgg
    36301 cgtttcggca ggggaggcac gggacctcat caggaagatg atccataagt acatggacga
    36361 cccgaacatt gcatcgctcc tccttggtgc ggtcctgctc gaggacgagc tctactggaa
    36421 acgggtcaac tccgagatca aggacgaccc ggtcttcctc ctctcagacg agatcatcgg
    36481 aatggcgatc gccgagtgca tcggtggcac ctacgcacgc ttcgagttca cccgttacga
    36541 ccagaagaag cccggcatac tcggggcact cggccccttc ctcgacgatg ccgtcgccgg
    36601 gctcatcgcc ggctgcacct caaggttcta cagcgaatgc cactaaccgc cctcgtctcc
    36661 ctgctccagt tcaccacggt cctcccgctc ggcagaaccc gcgacttctc cgcgtttgcg
    36721 gaacgctcgt ggatatatcc cctggcaggc tacgtggtgg gagggattgc tgcagccgca
    36781 gtcttcttca ttggcgaacc catggtcgca ggtgccctcg ccatcgcgct cctcttcttc
    36841 ctgagcgggt gcaaccacct cgacggtctg atggactttg gtgacggact gatggcccac
    36901 gggggcaggg agaagcgggt gggtgctctc accgaccgcc agattggaac aggaggggtt
    36961 gcgatggcga ttgcggtcac cctgctctcg ttcggatcgc ttgcggccgc tccgtgcctg
    37021 ccttgtgcac tcatcgtcgc cgaggtggga gggaagtttt caatggcatt cctctcgacg
    37081 ttcggcagac ccttcaggga ggggatccac tcgtcaatac aatcccattc aaagccatat
    37141 tttccggttc tcgcagcaat cctctgcatc ccgctcgtgc ttctccccat cgcgccggcg
    37201 tccctcgggc tcgcgtccgt catgatgatt gcctgcccca cggcccttct ccttgtttcc
    37261 cggaaactct ttggaggtgt gaacggggat gtgacaggcg caagcaacga gattacgagg
    37321 gcggcggtcc tggtctccct ctgcctgcta tagcaaggac gcgcatcttt ttcccatgca
    37381 gggcgcagtg tactaccatg cgcagggaga atatcgttca catgcgggga ggggtgatca
    37441 cagagaggga cccggaattc tgcaccatcc gcaccaggat ccccgcaggg gtcgtgaccc
    37501 cggcgatgct gcggggaatc gcagatattg cggattcctg ccaggccccc catctccacc
    37561 tcactacccg gcagaccgtt gagatcccaa gagtcgaccc cgggcggctc gaggagatct
    37621 cccgagccct cggtgagaac gggacaccga ttggctcaga gagggacgag gtggtgaaca
    37681 tcaccgcatg ccccggcatt gatcggtgcg tatttgcaaa tatcgactcg atctccctcg
    37741 ccgtggagat cgaccgccgc ctctttggaa gggagatgcc ggtcaaagtc cgcatcgcaa
    37801 tctcggcctg cccgaatgcc tgcacgagcc ccatgctgaa cgaggtcggg atcaccggcc
    37861 ggatcatgcc catacggaca ccgggcgtct gcaccgggtg cgggacatgc gtggaatact
    37921 gcaaggaaga ggcaatctca atcaagaaag ggatatcagt ccttgaccct tcgaagtgcg
    37981 tgcagtgcgg gacgtgcatc cgttcctgtc ccttccacct cctcaagtcg tccggggaac
    38041 actacctcat ctctgtgggc ggaaggagag ggagacaccc acgggtggga agggaactta
    38101 tcacggtaaa ttcacccggt gcggctgccg acgtcgtcga gaaggtggtg caatgggtat
    38161 accgccgcgc atggagcggg aggctcctct cagagcagct cgatgagatc cagttcgaga
    38221 agttccggga agagatcagg agccagatcc ccgcctcatc gatattcgag gcgcaagatc
    38281 cagatcagag ccggtaacgc ggcctgaatc cctggctctg atcgtccgcg cactcgatcg
    38341 ccgtgccgtc cctgacaagc tggttgaact ggagggcggc gaccatcagc gcctcgtcag
    38401 atgcagaacc cctcttcatc tccttttgca cctgctcctg gggggcagga tagacacgtg
    38461 ggccgacctt tatacccttg cagtgctcgc agaatgcgca gaatttgtgg acggatacct
    38521 cccctgtggc acattcgacc gtggcgcggt tgccgctcgc atccttggtt attggcaggg
    38581 tcttcatgct tccggaatgc attttatacc gtcaccgata tgattctgtc gataataccc
    38641 cggtttccgg cgaaatcgcc cagaatccgg tcaatcgaaa cgattaatat ggatcccttt
    38701 gcaatatttt atactcgtat aaatgggctg aactctgaca ggctcatttt tatcggagga
    38761 atgacatggc agctgaaaag cctcacatga atctggcggt catcggacac atcgaccatg
    38821 ggaagtccac cacggtcgga aggcttatgt tcgagaccgg gaccgtcccc gcgcacatca
    38881 tcgagggata ccgcaaggag gccgaagcca agggcaaggc cacattcgag ttcgcatggg
    38941 tcatggacaa cctgaaggaa gagcgtgaga ggggtatcac catcgatatc gcccacaagc
    39001 gttttgacac tcccaagttc tacttcactg tcgtagactg ccccggacac agggactttg
    39061 tgaagaacat gatcaccggc gcttcgcagg ccgatgccgc catcctggtc gtagccgcac
    39121 ccgacggggt catggagcag accaaggagc acgtattcct ggcaaggacg ctcggtatcg
    39181 gtcagcttat catcgcggtc aacaagatgg acgccgcgaa gtatgaccag aagcgcttcg
    39241 aagaggtgaa gaaagacctc tccgcgctga tgcagatggt cggctacaag ccttccgaga
    39301 ctctcttcat ccccatcagc tccctcgcag gcgcaaacat ctccaagaag agcgccgaca
    39361 ccccgtggta taccggcccg actctccttg aggcactcga tacgctgaag gaacccgaga
    39421 aaccggtgga caagcccctt cgcctgccca tccaggacgt atacagcatc agcggcatcg
    39481 gcaccgtccc ggtaggccgc gtcgagacag gggtcatgaa gaaggggatg aaagtctcct
    39541 tcatgccggc caacaaggag ggcgagatca agtcaatcga gatgcaccac gaggagatcc
    39601 cgcaggccct gcccggcgat aacgtaggct tcaacgtgcg cggcatcgcc aagggcgacc
    39661 tccgccgcgg tgacgtctgc ggccctgtcg aggctccccc gaccgtcgca gaggatttca
    39721 ccgcccagat cgtggtgctc cagcacccca gcgccatcac cgtgggatac acccctgtgt
    39781 ttcactgcca cacgactcag actgcctgca ccttcatcga gctgaagaag aaactcgacc
    39841 cgaggacagg tcagaccaag gaagagaacc cgacgttcat caagaccggg gatgcggcaa
    39901 tcgtccaggt gaagccgtca aagcccatgg tcatcgagaa cgtaaaagag cttccccagc
    39961 tgggaaggtt tgcaatcagg gatatgggat cgactattgc agcgggaatg tgcattgcca
    40021 ttaccccgaa gcagatgcgg tgaacccccg atcacctaaa tttttggtga gcatccatgc
    40081 aaaaagccag aatacgcctt acagggacag atttcaagaa agtcgagatg gtatgcgatc
    40141 gtatccgcga gatcgcggag cgcacgggcg tgaatctggc tggaccgatt ccgctgccca
    40201 ccaagcgcct ggtcatcccc atccgaaaga gccccgacgg agaaggcact gcaacctggg
    40261 accggtggca gatgcgtgtg cacaagagac ttatcgatat cgacgccgac gagcgggcac
    40321 tccgccagtt gatgcgcacg caggtcccaa aagacattgg catcgagata gtcctggaaa
    40381 gttgagggga gcggtttgac cgcagccggc attgctcaga gaatccggcc cctcttctcc
    40441 tttcatacgc tctttttcct gatagtactg ggtgggtttc tcctcaggtt cctagccctc
    40501 gatctcaagc tgctgcacca cgatgaggcc atccacgcgt ggttcgccta cgaactgctg
    40561 accaagggga cctacatcta cgacccgatg taccacgggc cgctcctcta ctatgtcacg
    40621 gccgggatgt tctatgtctt cggccagtcc gaccttgtcg cgcggcttct gcccgcactc
    40681 tttggtgctg ccatcattcc cctcgtatat gcgatttacc gattgggtta cctcgaccag
    40741 cgccaggccc tcatcgctgc gcttttcatt gccatatctc ctgacctggt gtatttttcg
    40801 cgtttccttc gccacgatat cttccagctc ttctttacgg cgctgattct ggttgcactt
    40861 ctcgcatacc tggagagggg aaaggtctgg tatgcgctgc tcgcgggact tgccgccggc
    40921 ggagggatgt gtctcaagga agacatgccc ctctttctcg tgattgtcgc cctctttggc
    40981 atatacctcc tatggaagca gccgcttccc atcccccgca cgtggaagcg agacatcgtc
    41041 ctcgcggtgc tcctggcagt ggcgatcatg gccgcgttct actcatcgtt cggggtccac
    41101 cccgagatcc tcgtgacggg gctggagaaa gcgtatactc actgggcatc catgcacggc
    41161 atgtgccgca tctgcggccc atggttcttc tatatcctcc tcttcctcct ctacgaggta
    41221 cccgtttttc tcatggccat cttcggaatg gtccagttcg ccgaccggca caacccggtc
    41281 cccgcctgga tagcccgtgc ccgcgcattc cttgcagcaa ggcgtccggc gaaccaggag
    41341 gagagcgaag cagtcatgac gccccggtct gcctctgtcc cggttgcctg ggacaagaag
    41401 gagatcttct tcctcttctg catcctgtgg tttcttgcgg cactgggcgc ctatgcgtac
    41461 atcggagaga aagtgccatg gctcatcatt caccagctcc tccctgccat ctttgtgtcc
    41521 gtctacctga tgagcaagaa gaaggcagtc tttgcactgg ccgggtgcgt cttcctggtc
    41581 ctgatgacct ggcatgtcgc gttcatcccg gccgacgtga acgagccgat cgtccaggtg
    41641 cagaactccg aggacatgag gcttgtaatg gcattgatcg atgtgtcgga ctcagtggcg
    41701 atagcttccg agaattactg gccgctcccc tggtactacc ggggagaggg atggtccagg
    41761 atgcatttct atggaaagat tgtggatgag tacaccatct atcaggtgaa ccctgacatg
    41821 gtgatcactc acgaccaggc cagttacgcc tcgcttgacg ggtacgagaa gcatacctac
    41881 aagctctcct actggttctc gatatacgac aacgagaacc gcctccccga gtattacgtc
    41941 aagcgggacg ggaagatggg aagtatcaat attgacgtgt tcacgaaacc aggactttat
    42001 cagaaagctg ggattgttcc ccccg
//
LOCUS       DAOQYR010000010        41198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_1070_length_41198_cov_8.734560, whole genome shotgun sequence.
ACCESSION   DAOQYR010000010 DAOQYR010000000
VERSION     DAOQYR010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 41198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41198
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1070_length_41198_cov_8.734560"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PKM87_02310"
     CDS             <1..558
                     /locus_tag="PKM87_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNQ28929.1"
                     /translation="KNVQEKDMGAVKGTNLRDLCELVGGMSPGDTVKVRAADGFSREF
                     AYQNVYSFPPRQGPLVVTWWKADAGYVPDYREGMRLVFFADTSTNPWGIHAFGNWDWH
                     ESADEEHWYYYRQGDQKYPTTTGLSVQYISDILIYPGSAAQAEPRRMAIPNAPEAPTQ
                     AGFSLHVIMGALTAGVLLVYATKKR"
     gene            624..1466
                     /locus_tag="PKM87_02315"
     CDS             624..1466
                     /locus_tag="PKM87_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNQ28930.1"
                     /translation="MFPCIIAAILLFGLIHVTCAAPTTEVRAAKYAADGTTVLNETKV
                     SYQWMEANLQVYGDGLTHYFHQGPVFVSDKEGQWDVNETTNFKDMGAVRGTSVRDLCD
                     LVGGMNPGDEVMVKASDGYHVEFPYENIYHPLPRQGSVVVCWINGEETAVGERQGTGY
                     PPSYHVGMRLVFFADNSTNHEGKNVFGNTDMRETMPPDSVHLFDNLYPSTSGYTVKWV
                     DEVRIYSGGYHGSSELLPKSYESKMDETYPPPPTRSPLLLGIPLVVCLACALMLVKRK
                     DRES"
     gene            1463..2044
                     /locus_tag="PKM87_02320"
     CDS             1463..2044
                     /locus_tag="PKM87_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNQ28931.1"
                     /translation="MKLTIPVLLISFLAVGFLMAAGCSDTKSPVAEAAPDYSDWRLTL
                     KGASEMVFTLDELRNLPSVNGHGYCVSTTGFRFGPYRCKGVDLRILASMAGGMEPGDT
                     IWISAPDGYLWVFDLDQMEGRGFVTFNEGLAEIPSPPLRIILMYEQDGKPLSYNDGGP
                     ARIAIISEEPGVITEGSAWVKWVDRIEIKEKTA"
     gene            2041..3036
                     /gene="wtpA"
                     /locus_tag="PKM87_02325"
     CDS             2041..3036
                     /gene="wtpA"
                     /locus_tag="PKM87_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tungstate ABC transporter substrate-binding
                     protein WtpA"
                     /protein_id="HNQ28932.1"
                     /translation="MNRAIVPVILMLIFCTLYSGCSTHPNTGKIPLKVVAAGSLLVPF
                     SELESQYEALNPDIDVQVEGHGSIQAIRQVTDLYRKFDVVAVADESLIPDLMYRPSRE
                     TGKNFTDWYVPFARNEMVIAYTEKSLYHTEINETNWPEILAKPDVRVGFSNPMLDAAG
                     YRALMVTMLAGDYYGDQTIFPRVIGNHFDPPLVMENSAQGYTVRLPEVPHPSDQHIVI
                     RDGSIFLLSLLAAGGIDYAFEYRSVAEAQNLSYVNLPPEINLASTDYEDWYAKSHVIL
                     GFPRFSSIGQERGGRPIVYAMTVPSDATHPDEGQKFLQFVLGHEKIGERGWPASL"
     gene            3558..4724
                     /locus_tag="PKM87_02330"
     CDS             3558..4724
                     /locus_tag="PKM87_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ28933.1"
                     /translation="MQPHRTAFFTDGYIHHTFLLENLADATRYEYRIWSGETEYGIYH
                     FLTYPCSGPVTFVVYGDTRDELPRISQEERHRPVADVIAAEPGVSLVVHTGDLVNNGE
                     NMSDWDRFFATGAVMFANVTLVPVMGNHERNSDLYFEIFRTSPNYTVEAGNILIVVLD
                     SNDWAWNDLPAQSAWMNESFSRDVPWKFVVLHHPIYSSEEKHPGGFQNLRREWEPVFR
                     ACGVIAVFQSHVHVYERDVAHGITYITEARGGAPFYTLAEQKIPEYRVSSENTLGYTV
                     VRTENNSDFVHMRVMAVSPAGGKGESRASIIEEITLPEPVEWADDDSVDKNQRSLFPA
                     RLQTLQTCRDALFAGSGEACSGAEECRNLISSLFNPEEEDGHLLQTRDIPERKL"
     gene            4781..7978
                     /locus_tag="PKM87_02335"
     CDS             4781..7978
                     /locus_tag="PKM87_02335"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNQ28934.1"
                     /translation="MISCVLVGASGVTSAATTEIRVVKYAADEFTILTEKTVDYRWME
                     KNLRVYGDGFTHYYHQGPVFVDDPDPVKEEELRWNPDEDTNVQEKDMGAVKGTSVKDL
                     CDLVGGMGSEDRVKIRSADGWSMLFAYKNVYEYSSREGPIVICWSKNGMFPDTGYSDG
                     MRMVWFADTSTNPWGIHAFGNYDWHEAADSEYWYFYHSGDERYPTTTGLSGQIVSEIL
                     IYSQEEPAGSIEVTSTPAGAEIVLDGEETGEITPFTLVDIPAGSHTVSVQKDGYLDPI
                     EEWVDVSHGVVVPVHFNLEREYSVTSSSGDLASTQDGGYVSEQISFGLVATAITGNIS
                     LHVAPGERQICRSGQSITYRIPPDSLPPSSFAWARLYLFSDAGYTHEQRIEVGINGMS
                     PGTPERSTFRLPDGKVTETLAFNVTRMLRTDAPMEISARNPPGGNEWTLYPPLLLVVA
                     GDDGDVHRENWISEGAGLVHAMKEYSYQDLPNLTVSDFPDIRTGLSGAELQAVSTIPD
                     DGGASSPMAFQNSVVIPGEYAHNHAGIWVSRYNVTGTVSEYTHNELFWKTTPGRDPAV
                     IGPRIVILTVSYPPGLSTTDYTSKNRSAIGPIDTLSPVSESPPPSPSTSAVTTITPDL
                     IGNGREEAIRDDSFFSRIWEVLFWLLGMPIPGHGDQGQAHVTGDSPKGDLPNVESPSH
                     APDSSSGYPLQVSTTPPGALVSIRGGSDVQISPATFVLPGGEYRLAAEMDGYLSCQTM
                     VRLAGPQEITLVLTPEGGTDESLPGEAPERSRHGGLLIVTYPGDLELSVDNQQMERKS
                     PLMLYGLKEGYHTVKATRPSASAGKGETLITRAWVYHDALAICELDFVVARLDRRIRI
                     TDPSGNSTAFTLNGIYPLLRTPVTLEVPGTGSYITLIGESTYTNIPIQDGLADGSELL
                     LPTYNGNYHAISIESAPSGAEIFMDGARTGNTTPSLVRGLSEGTHLVMVSLPGHLPTH
                     RVISIPRTQEEYIKGTVSFILETYPSGPLRVESVSAGAAIFIDGIATGEKTPFSFTGI
                     PIGTHELTLRSGDTTRSRDIIVRPDNPNRYIVHLL"
     gene            8171..13210
                     /locus_tag="PKM87_02340"
     CDS             8171..13210
                     /locus_tag="PKM87_02340"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ28935.1"
                     /translation="MRSKYALTLCSFLLIALVIVPACAATTQLHVIRYANDNTTVLNE
                     TTVTYQWLEEALPVLGDGSTHYYHQGPVFVNDPDPDIEEQLRWNPAEDTNEKDQGAVK
                     GTNLKDLCDLVGGMSPGETVELEASDGFSKRFAYENVYSYPIRQGPMVITWFKDGTYP
                     DTGYSDGMKLVFFADDSVNPWGYHMMGNYDWHESAAPEYWYYYTSGSEQYPTTTGLSV
                     KYISDIKIYSQEAPPVPVDTLFDGTVTLIEGETFTVTAYNNASGNYTVDYTTPLGALE
                     TASKAGSGFTYDITDKNYASSGALLLDNIGSYIYQKTPRKAWYAYVNDVYKDGYNNPA
                     SALNLIPLNDGDTVEYYFVDGTVADPTDYAAVTAAATAAVKIIADIQPAGPVVDTLFD
                     GTVTLTPDTTFTVTAYNNASGNYTVDYTTPLGALETASKAGSGFTYDITDKNYASSGA
                     LLLDNIGSYIYQKTPRKAWYAYVNDVYKDGYNNPAGALNLIALNDGDTVEFYFVDGTV
                     ADPTDYAAVTAAATAAVKITVDISGSTPTEPDWELTLTGARDQTVTKTYFEQGLACPS
                     SGHMVSWTDAEGDEWSGVPFWLLVAMVDDDPDSGPDHYNFNDALAAEGYSIKVTADDG
                     YSVNFESADIARSDDYIVANALNGEPLPLLRPNSTKPCFPLQLIGPAIISGKMIGNIS
                     SIELIGLPKPPEGWTLTMEGDVVDAISQAYFEEAMDCIHPASWTDGTGNVWTGIPLWE
                     IVGSVDDIETAPHWTFNDDRAAAGYTIRVIAGDGYNRTFASGDVARSGNYLVANTKNG
                     APLVGTEAPLRLVGTNATGGKSVGNIATIRLEGLPAYPAGNWSLLLDGAIRDTIPEPE
                     FEDWAACHSATYDDGTGNVYEGIPLWRLMGWVDDRIPHGPNGFNNALASAGYKVIVTA
                     GDGYSKEFTSQEIGTTNGFIIANRINGQTLPTDGAHPPYPLRLVGAGLPSVHYSVGNV
                     VKIELADFQEPTEIPQIHIIKYDADGVTIINQTTLDHIWMEANLPVIGDGVTHFKYQG
                     VTFDPGDLWDPTETKGMNPPKIDNAIKGTKVRDLCDLVGGMEPGTEVTFVATDGWETT
                     LPYDCIYTNPHVYSHLGETIIAWYADGQYVPQYGDGPRLFFTPEDHVAGQWNMHEALA
                     QQYWHYYWDGGIQYPSVAGLSAKYVDTIKIYASPVTDWTLILDGEAIEGLNQTISRNY
                     FESALACQFGADHKASYTDSQGRNWEGMPLWFLCGFVDDPDQHSDNAYNDTKALDGYR
                     IIVTGSDGYNATFNSQDTIRNANFIVANTLNGTRIAEGDSSWPLRLVGQNVTGGKVVR
                     SVASIVLAPLAEPPVADFEASPLSGNAPLLVQFTDLSTGNPLEWAWDFQDDGTIDSTA
                     QNPSCLYQDPGTYSIRLTVTNAGGTSTKTKSQYVTVIVGPPIADFSASPVTGPSPLQV
                     QFTDLSTGNPLEWAWDFQDDGTIDSTAQNPSCLYQDPGTYSVRLTVTNAGGTSTKTKS
                     QYVTVTVGPPIASFTASPRSGNWPLTVSFTDTSTGSQIQSWEWDFGDGKKSPKRNPIH
                     TYLKPGVYTATLTIRYPEGSDNASETIEVKGDFTASAVIKTPVALFSQSSVIGTPPLS
                     VSFTDLSLKNPTTWLWDFGDGGASNEQNPSHTFDKAGMYTVRLNVTNDYGFSSAVKRV
                     FVTPILSFFSR"
     gene            complement(13253..14350)
                     /locus_tag="PKM87_02345"
     CDS             complement(13253..14350)
                     /locus_tag="PKM87_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HNQ28936.1"
                     /translation="MTRNISFNIRELAGSVGDFGTILPLILAVSAVAGLSLGYILLFF
                     GIWFIITGYYYQIPVPIEPMKVIAVVAIAENMSAGQIAAAGLILGLLFFVLGYGKWLD
                     ALEKYIPQSVIRGIQLGLALLLVKTSTGFMTGDLLVFGLAVLLMAAVYVIARFTEIPD
                     LSAVILIGVAFGTGVILHGFPAMALLPPPHLVIPSMSDIPAALGTLVVPQALLTITNA
                     ILATSLLTRDLFGQEVRPARLSRTIGLMNLTTVPFGGFPMCHGAGGMAGQYRFGARTG
                     GANIYAGIFFLMLALLFASPDVLSLVSGGFYAALLVFVAIELGRHGIKTDSYLVTGLI
                     AVLSVLVNMTAAFAAGICLAYGIRYVKKRIS"
     gene            complement(14387..15208)
                     /locus_tag="PKM87_02350"
     CDS             complement(14387..15208)
                     /locus_tag="PKM87_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridylate kinase"
                     /protein_id="HNQ28937.1"
                     /translation="MHRKRVERFEVANRLQGESLVRESLLRSFEGGEQIRLVPDLNVV
                     KIGGHGIMDYGKEVVLPLVEEIGDLSKEHKILVVTGGGVRVRHILDLGLDLGMPTGVL
                     AELAGKISEQNAIMMSLLFSRWNGMRIHTADLLELPMLFHLGVLPVTHGTPPYGLYEQ
                     PAEIGTIPPHRTDTGAFLAAEVLGAKNLVLVKNVDGLYTANPFLHEDAERIPDITASE
                     LLDMNLDDMVLEKKVVELLLYAKNVHEVKIVNGHTPGNVRKVLSGGMPGTIIRAR"
     gene            15329..15532
                     /locus_tag="PKM87_02355"
     CDS             15329..15532
                     /locus_tag="PKM87_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28938.1"
                     /translation="MKIYVRERQKVREGAKQPMFRIVAVTGGQVQIELTHFRKFEIEQ
                     IAKDIGAEVVYLQEIPDEEKKKA"
     gene            15638..15994
                     /locus_tag="PKM87_02360"
     CDS             15638..15994
                     /locus_tag="PKM87_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28939.1"
                     /translation="MAALSIDLIFTAAIRIFLEEEKAITISYSPGEVRIRFPTTRRLA
                     EFLEVPHYYVLPYFAMMEEMELVTRAERVGILTTSSGTRKLITMMSKDFRPQASAVMG
                     EPILAYLESSVSSQLV"
     gene            16134..16820
                     /locus_tag="PKM87_02365"
     CDS             16134..16820
                     /locus_tag="PKM87_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ28940.1"
                     /translation="MNEIIEGFVQAGELIIRLDPEVLEITARSLLISLSATLIASLIA
                     IPIGGFIHFREFGGKRGLITIIQTLYALPTVIAGLFVFLLLSRVGPFGFLGLLFTPTG
                     MIIGQTVLVIPLMVGMTLIALSGVKKTIQDEVISLGATSSQIIWTIVKEARFAILGGV
                     ILGFGRAISEVGAAMIIGGNIRGYTRILTTAIALETSMGNIAFSIALGIILLIIALVV
                     TTALFLVQER"
     gene            16822..17901
                     /locus_tag="PKM87_02370"
     CDS             16822..17901
                     /locus_tag="PKM87_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ28941.1"
                     /translation="MIEFVHVDKRFGEKVVLKDLTFRIEKGEIFTFIGPSGTGKTTIL
                     RLIDLLENPSSGSVLIEGNDTKVSEGERVALRRRMSVVFQKPAALRGSVYENVALGLK
                     FRGTPADEMRVRVTEALGLVGLDGYEERKATTLSGGELQRVAIARALVSRPEILLLDE
                     PTANLDPASTERIENLISSINERFGTTIVLSTHDMIQGQRLATRIAVILNRTIGQVGD
                     SHQIFYQPKSKEVARMVGVENIFDGVIAENTGGLATIDIGNTCIVAICPYPEGTRVTS
                     YMRPEDVIFLIEKDGKSTARNEIEGTISRVTPMGPSVKVRMDGGIRVVAVITRRSYDE
                     LGLSPGAHVYASFKASAIHVVKNEP"
     gene            18084..18872
                     /locus_tag="PKM87_02375"
     CDS             18084..18872
                     /locus_tag="PKM87_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ28942.1"
                     /translation="MRCIALASGSKGNCLYIEGSSSSLLIDAGLSRREILSRLHSVGG
                     STEHLSAILVTHEHIDHIASVEALARHLSIPVYATGGTLLDLLDSRPVRRNPIRAVRV
                     DYGERFTIGDFSVEAFEVSHDAREPCGFLVTEGDLTVGCCTDTGMVNDHMMDCFCRCD
                     ALVLESNHCPEMLKNGPYPEVLKRRIRSRRGHLSNPDAARCVNELCTRVHMIMLAHLS
                     EMNNTPEKALSTARQGAGLFSTDIEITVATNPGTGQGWPHGIRL"
     gene            complement(19147..20340)
                     /locus_tag="PKM87_02380"
     CDS             complement(19147..20340)
                     /locus_tag="PKM87_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28943.1"
                     /translation="MPEEWIPDALVEQKMGQTKREMMEYVEKNSEKIDSNIPIFFGHV
                     VSILEKNYPDIPDQAYDRFIDDVTVKVLEESRHSTDIEYIEKLFRHATRVKRKYTGRA
                     IFDIVLGIKLIDMGKYSDAIEVLKKYRTVDAMVCTAIAFSYYELSSLELPPDKKPDKA
                     TTSAMALSAREQMIELARLRPPVNRLRFPQVVQELRVNKVFWFMIRLAIDWFPNEPEY
                     LKIGLEKAKKDGNREMRGELLKIASERYYDDMFFLRDLYHFKLEGRDAGGATAVVRQM
                     MQQHPDELEPIYYGLQLSIISSQESAYQKFRSTAISKKMPAHIILMLDLSFMIITGRK
                     ADAEVMLEDTKKKLPALGYYLGVLEYLMGDIFSEDEKRSRTAKKLFLDSIDMYSLKLL
                     KMKEG"
     gene            20485..22149
                     /locus_tag="PKM87_02385"
     CDS             20485..22149
                     /locus_tag="PKM87_02385"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HNQ28944.1"
                     /translation="MKFAEFARICEEIEGISGRLEMADKISGILPGLDSRDLAIFVHF
                     ITGKIFPDWSPLKMGIGPNLLYDAVTLIVDGTRSDVIGEINRRGDVGLAVQVILGQKK
                     VSRRSPRTATIQTSLFSQELDLFGVYQKLEKIARQGGRASQQEKLSLIQSLFQNASPL
                     EARYLARLLMEDLRIGVGEGTIRDAIARAFSVDPSNVEHAYQARNDLGEVAILARAGE
                     NALRDVRILPFHPVKMMLAQAGDIHGAIAENGAMAAEFKYDGSRFQFHKAGGESRIYS
                     RKLEDVTDALPDILSALYGATTHDVILDGEVIAVKDGKPLPFQAVLRRFRRKHGIEEM
                     QKTVNVQPFVFDILYLDGETLIDLPFRERRARLEATVSDYVTPQLVSGEPDAIERFYK
                     GSLAAGHEGLMLKVLSAPYTPGIRGKNWLKIKPSVDTLDLAVIGAEWGEGKRAHLFGS
                     FLLACQDQGELQALSKVATGFSDEQLAELYDLLRDHVLSESGKEVQFEPILVFEVGYS
                     EIQESPNYECGYALRFPRFIRVREDKGIADIETLDGIRERYIRQKK"
     gene            complement(22340..23452)
                     /locus_tag="PKM87_02390"
     CDS             complement(22340..23452)
                     /locus_tag="PKM87_02390"
                     /inference="COORDINATES: protein motif:HMM:NF021416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2117 domain-containing protein"
                     /protein_id="HNQ28945.1"
                     /translation="MNKKDDTATHPCQVRTDTPGDRTKNSDIRQMVMVLHGPEVFDSG
                     NAEWLSRVLAPKKIVVAGVMARAAAEESGLPCEFMSVPPSVAIRMLEAPSFLANEGKT
                     PESGRIFGEIVASRVGGEGLIQVECARREVICWNRKPDAAVHKISGLTGYPVVERMAP
                     ALMPDEGYRTIRGCLPGEAVFVNGVVIGTATGREVVIRSEGEKIFPLSGLQVKDHGLE
                     KLERAGPVDVSRAWCKSGRLRSKGPLAGPRHAIVGKVIFVDHCGHHLYRMIGNGNICG
                     MVTVGDDTTAVCGHIGAHLGIPILGIVDGDSDNIVPERYAKGSILAVAQGISDDELGR
                     EVAGIVPEGEASWEECTARIIAHIGDRAVIRKPPEP"
     gene            complement(23579..24934)
                     /locus_tag="PKM87_02395"
     CDS             complement(23579..24934)
                     /locus_tag="PKM87_02395"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HNQ28946.1"
                     /translation="MESIKSVILAGGVGTRLWPLSRTYNPKQFLKVNGTSLFQDTWRR
                     ALLISGPEEVWVVTNEAHQFLVRSQLEEVGVHVKDERILKEPVAKNTLPAITWAMQVI
                     REEYGDSVVAVFPSDHNLENGAMKEIAAARCISDKYLVTFGVPPTTPHTGYGYIDPGD
                     PLALGARVRKFREKPDWETAKVYLEAGFLWNSGMFLFSTGVFFDELAKYQPELYSQFR
                     DGIREYANLPSLSVDYGLLEHSEKVAVVPLKSRWTDLGTFRAWYELRPHDDYGNAGHA
                     EMLDSERNFVHAPGKRAALIGVRDLVLVDTGDALLACHMDMTEKVSELVKRFRDRGDT
                     ITDYHLQVHRPWGSYTELERSRFFRIKRVTVKPGKRLSLQMHHHRSEHWIIVSGMADV
                     IVGDRTIHLRQGESTFVPAATRHRLANTGKIPLEVIEVQIGEYLEEDDITRFSDDFNR
                     V"
     gene            complement(25021..25554)
                     /locus_tag="PKM87_02400"
     CDS             complement(25021..25554)
                     /locus_tag="PKM87_02400"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HNQ28947.1"
                     /translation="MKQAKAGPNILITGAPACGKTTLVEGIAQSLAPIRPEGFVTREV
                     REKGERRGFEMVSFCGKTGILADISIRSGWRVGKYGVDVAAFDAFLEELHFDESPFVI
                     IDEIGKMECLSSLFCMRILDLLDSFPVLLATIALRGTPFIESLKCRDDVEVFCMDSHT
                     RDQVTKHVIQRLRSALD"
     gene            complement(25570..26304)
                     /locus_tag="PKM87_02405"
     CDS             complement(25570..26304)
                     /locus_tag="PKM87_02405"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ28948.1"
                     /translation="MRWRPILGIMALLVILALHVAPVLAEDCGCGGDSGSGDAGDSSD
                     GDSSSGYGSSQDPAAMAATAQDLYMSGHYFEALQAFNDSLAIDPYNAPALMGKAEVLF
                     TLRQYPEAAETYRKVVTISPGHDRAYFCLGNTYLVMGEYQEAAEAYERSLGIRPGNTL
                     AEENLRVARSFMMERVPTEVPTIGISVAPEHETVQATMPPASIPPTDPTTAHSAPMLD
                     GWTGVLSLILSVTIAIAGMKKRTNGA"
     gene            complement(26434..26811)
                     /locus_tag="PKM87_02410"
     CDS             complement(26434..26811)
                     /locus_tag="PKM87_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ28949.1"
                     /translation="MTKSILVVEDDPVISQLISWRLKKLGYEVSGTAPTVEKARSLLK
                     SKVPDLVLIDIALQEGVDGTVLGSQIRKEYCIPFIYLTAHSDNETLSRAIETGPSGFL
                     LKPFKDEELRVAIEVALKQVQGA"
     gene            complement(26896..28734)
                     /locus_tag="PKM87_02415"
     CDS             complement(26896..28734)
                     /locus_tag="PKM87_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ28950.1"
                     /translation="MVDDEASLLDVTKIFLEKGGAMEISTALSAHEALEMLKTRGFDV
                     IVSDYEMPQMNGIEFLRAIKETHPHIPFIIFTGRGREHVAIEALNLGAAFYLQKGGHP
                     RSQFAELRNMIEQAVSRKRAELALQRSEQRLSDIINFLPDATFAIDREGRVIAWNYSI
                     EEMTGVPAREILGKGNYEYSIPFYSERRPILIDLIFESSEEIVKRYYGVVHKKGDLLI
                     GETDLPRLGGQKAILWGKASPLYDHEGNIIGAIESIRDVSERRRMEEALKESEARYKG
                     IVESQEDLIARFLPDGTYIFVNPAFCRYYELSYDEIVGSRFRPHVPPEEMPLVRKHFA
                     SLTPKNPTGYIQHRIILPGGEVRWQSWTDQAIFDEKGGIREFQSVGRDITDVVKAEEA
                     VQMANRKLSLLSRITRHDILNQVTALMLYLDMAKEQAESPWLVDFIEKIQRVGQNIER
                     QVAFTKEYEDIGVRAPTWQDMNGVLQRALQSFDLSAIRMHCEVGDLEVYADPLLERVF
                     HNLVDNAMRHGDGITAISISTTMSSYGLILSVEDDGSGIPHEEKEKIFEKGYGKGSGF
                     GLFLAREILDITGISLIETGEPGTGARFEVFFPKGTFRSLKRSNAP"
     gene            complement(28835..29248)
                     /locus_tag="PKM87_02420"
     CDS             complement(28835..29248)
                     /locus_tag="PKM87_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF61 family protein"
                     /protein_id="HNQ28951.1"
                     /translation="MYDESVLRRWMSLEMGKINDGVVSARVPLVSLLEMEKPMAVNRG
                     GREHHFDREVIRKIGAPLPVNLRSSLKLPIIFYFDMEVSNSCMLADEVAIAVLQELGD
                     LSRERRIEGGRMWVARALVFALLRKYPTAVQIMMR"
     gene            complement(29574..30425)
                     /locus_tag="PKM87_02425"
     CDS             complement(29574..30425)
                     /locus_tag="PKM87_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNQ28952.1"
                     /translation="MDISAIRKRVYLILEPTYKGDRISYWFDVFITAVILLSISVMVL
                     SSVSSLSARYSLLFKIVFLFTTLVFSIEYPLRLWSCTSNPSYSHPVRGRLAWALTPFA
                     LIDLIVVFPFYLNIFTGINLTGLVVLRVFRIFKLVRYSDSINMIVRVVKAQRDMLITA
                     YLVLFIALISASTLMFQIEQPGQPEVFTSIPATMWWGIVTLTTVGYGDMVPMTAPGKI
                     LGGCITLLGIGIFALPAGILASGFTQERERRTNAKKKEQQEHHGEVYLCPHCKAEIRE
                     SDLWRKK"
     gene            30600..30809
                     /locus_tag="PKM87_02430"
     CDS             30600..30809
                     /locus_tag="PKM87_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28953.1"
                     /translation="MDIISLAGFLALLIIGIALVLFLIKIVWSLVPAAIVALVVYLFT
                     ASLWWTGAAFLAVVILTFLYKILAK"
     gene            complement(30844..31476)
                     /locus_tag="PKM87_02435"
     CDS             complement(30844..31476)
                     /locus_tag="PKM87_02435"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ28954.1"
                     /translation="MALKRIHEERYDAIVSDYMMPGMDGISLLKEIRKDGDDVPFIMF
                     TGRGREEVVIEAFTSGATFYIQKGGDPLAQFAELSHKIRQAVDKRRAEEMVRQAQWEW
                     EHIFNAVENPIVILSPDHRVLAANRATREALGRTAPELLGQRCYELFHHRESPPPSCP
                     LEAMITSKKYEAMQMEVEAMKGRYLVSCTPELDCDGNLIKVIHISTLING"
     gene            complement(31467..31661)
                     /locus_tag="PKM87_02440"
     CDS             complement(31467..31661)
                     /locus_tag="PKM87_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28955.1"
                     /translation="MRVGIRIKPCVFFFSGEEYTWGGLEFMISLLYIDDEPDLLELGK
                     RFYSGKRIFQSIPLIPRHWH"
     gene            complement(31793..31866)
                     /locus_tag="PKM87_02445"
     tRNA            complement(31793..31866)
                     /locus_tag="PKM87_02445"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31830..31832),aa:Val,seq:gac)
     gene            31999..32310
                     /locus_tag="PKM87_02450"
     CDS             31999..32310
                     /locus_tag="PKM87_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HNQ28956.1"
                     /translation="MADMNVSQLFLYEHDAGRIELLVRIVYWIAIGIVAWIYGLITII
                     CLVVQWFHILILGRRSRGLSDFAKGYLEYMVHRMPYMYIMTDRRPAIMPDTVKIYEET
                     G"
     gene            complement(32398..33999)
                     /gene="serA"
                     /locus_tag="PKM87_02455"
     CDS             complement(32398..33999)
                     /gene="serA"
                     /locus_tag="PKM87_02455"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNQ28957.1"
                     /translation="MTFRVLVSDPLAEEGLEILRQECTVDVKTDLGEDTLVSIIGEYD
                     ALLVRSGTEVTSRVIEAGKNLRFIGRAGAGVDNIDMDAATRKGIIVANAPEGNTLAAT
                     EHTMAMMLSLARSIPQANASLKKKEWKRSRFMGVELNEKTLGIVGLGRIGREVAKRAQ
                     AMGMHVVGYDPFISKDRAAQLGVEALSLEDLFKVSDFITVHTPLIKETKHIVNTKTIA
                     TMKEAVRIINCARGGIIDEKALYEGIKSGKVAGAALDVFEEEPPLNSPLLELDQVITT
                     PHLGASTVEAQQNVAVSIARQCLSVLKGGSARYIVNAPIVPAEHADVIEPFAILAHKM
                     GRFLTQMVGGRMEKVELTYSGELATLGNNTRFITLMALKGLLDPILQVPINIVNAEFI
                     AKERGISVSETMTPDSGGWKSLVTIRVKTDTMEETMSGTVFYKGRSRIVAIGGYTMDM
                     IPEGYVIVSRHLDKPGVIGRASTILGKGNVNIAGMQVGRIKPGEEAIMVLNVDSEVSS
                     DMMDEIRSMPGIFTAVFAKISDTLI"
     gene            34145..36415
                     /gene="ppsA"
                     /locus_tag="PKM87_02460"
     CDS             34145..36415
                     /gene="ppsA"
                     /locus_tag="PKM87_02460"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNQ28958.1"
                     /translation="MKKMPDILWLEEIRKEDIAAVGGKGASLGEMSAIGLPVPRAFVV
                     TSNAFRRFLIESGIEKALFAGLANLDVEDSGELEKAANKAKELVMKARVPASIKQKVK
                     EAYLKMEGGLMVVAVRSSATAEDLPDASFAGQQETFLNVKGEKNLLLAMQKCWASLYG
                     ARAIYYRVKQGFDHQSVNIAVVVQQLVNAEKAGVMFTSHPVTGEPLSIIEGSWGLGEA
                     VVSGSVSPDKYVYDQRSEKVIDRLIANKRYQILSDGDNGTKTVEVERERQDTPLLSDE
                     EVRKLATFGRIAETHYDVPQDVEWAIVGDDIYILQSRPITTIKPPSASTPAHEAGAGK
                     IILQGQGASPGIASGNVVIISDAKDTGRVREGDILVTRMTNPDMVPAMRKVAAIITDE
                     GGMTCHAAIVSRELGTPAIVGTKTATQVLKTGQTVTVDGEKGFIYEGRLEVPREKAQA
                     AVTAAVPLITATSVKVNVSMPEAAARAAATGADGVGLLRIEHLILGLNKTPGWFIANN
                     REEEFIQELYNGIKTVLDAFYGKPVWVRTLDAPTDEFRNMEGGEHEPIEHNPMLGWRG
                     IRRDLQSPDQFRLQVETFKRLWTQGYDNLGIMFPMVSHPSEFTRAREMMQQWGVDVES
                     ATLGVMIEIPSSAILIEDFIRAGIRFASFGTNDLVQYTLAIDRNNEHVAGMYQPKHPA
                     VLALIDSAIRFCREHGVEVSICGQAGSEPPMVKWLVEHGITSVSANIDAIAKIRETVA
                     RTEQQILLESARSRNA"
     gene            36408..37505
                     /gene="mfnA"
                     /locus_tag="PKM87_02465"
     CDS             36408..37505
                     /gene="mfnA"
                     /locus_tag="PKM87_02465"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase MfnA"
                     /protein_id="HNQ28959.1"
                     /translation="MREIGLSEESLFSFLLSKREEDVDHAHILSSMCTIPHPVAVRAH
                     VQFMETNLGDPGLFPGTASLERLLVERLGTLFQLSGACGYATSGGTESNIQALRMARQ
                     LGPKEKPNVIIPESAHFSFKKACDILCLEMRAVPLDENYRMDPVAAAEMIDRNTCGIV
                     GIAGTTEYGMVDPIAELSKIAGDHGVFFHVDAAFGGLVLPFLDDAPAFDFSLPGVSSI
                     AVDPHKMGMSTIPAGVLLVRDSESLCSLSVDTPYLTVKQEFTLSGTRPGASVAGALAV
                     LEYLGIQGLRDIVAGCMKNTRRLIDGMEAYGIMRAATPDVNVATFSPARIPPPWKVSW
                     TRRGDLRLVCMPHVHAGAVESFLKDIGEQNA"
     gene            37498..38046
                     /gene="hpt"
                     /locus_tag="PKM87_02470"
     CDS             37498..38046
                     /gene="hpt"
                     /locus_tag="PKM87_02470"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine/guanine phosphoribosyltransferase"
                     /protein_id="HNQ28960.1"
                     /translation="MLERLVASLETCPLVKRGEYNYFIHPITDGVPEVDPHLLREVCM
                     AMIKALDLEGVNRIIAAEAMGIPIGAVLSLATDLPMNIVRKREYQIPGEVAVHQATGY
                     SKGQLFLNGVKRGDRVVIVDDVISTGGTTRALLWALDQVGAEVRDICFVINRGSPDIG
                     RPYKTLVSVEVTDRVRVIDRLF"
     gene            38030..38971
                     /gene="dph2"
                     /locus_tag="PKM87_02475"
     CDS             38030..38971
                     /gene="dph2"
                     /locus_tag="PKM87_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide biosynthesis enzyme Dph2"
                     /protein_id="HNQ28961.1"
                     /translation="MTGYSDLAERLRARGARAVALQFPAGLKRKAGVIAQFLKEQGFE
                     VIVSGDPCYGACDLARETLGYADVLVHFGHTPLGDPDPRVIFEPYRVDFDPEILKNAI
                     PCLKATRVGLVTTAQHLHLLDAMKTVLAAIGVEAVTTRGTRGCGEGQVLGCSFGAARV
                     IGTEEILYVGTGVFHPLGVQLATGLRVVALDPFTGEVQEVNAEKFLRRRHALIEKARS
                     ADQFGLLVSTKTGQERYEMARGMAARCSRAILILMREISPEELVNLGFPAYVNFACPR
                     LAYDDQVRFPAPVLTPQEFSILLGECLFSDYRIDELT"
     gene            38977..39594
                     /locus_tag="PKM87_02480"
     CDS             38977..39594
                     /locus_tag="PKM87_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="METTL5 family protein"
                     /protein_id="HNQ28962.1"
                     /translation="MRLKRLEMLLSRVKGFPLPSARYEQYLTPPDLAARLLFHAHMRG
                     DIAEKRVCDLGCGTGILAIGAALLGAAEVVGVEFDPRALQIASGNGKELDVFPRFIEL
                     HLPNPDLASLVGPCDTVVMNPPFGAQEPHADRPFIDAALSIAPVIYGIFNAGSRDFVK
                     AYTEERAKVEEVVGGTLSMKRTFSFHAKDIRETPVEIIVLRRRMN"
     gene            39591..40757
                     /locus_tag="PKM87_02485"
     CDS             39591..40757
                     /locus_tag="PKM87_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ28963.1"
                     /translation="MKSLTGPIFRLFLAHMVTDLYMPVIAAILPLLISTRGYSYLLAG
                     LLITSYNLTSSASQPVFGWLSDRKGWQVHISVSVLISAVFIGLMGVVEPYPILIACAA
                     LAALGHACFHPHALTMVSRLATSLNRGKVTALFVVGGNIGYALGPILAGAVIVTFGLP
                     GLPLLVIPALVMAVVLHKSSPHPGEHPASLESPAKDPVSLASFSPVFILFSASTLRAW
                     AVFAAIAFLPPFLVLRGLDIFTANLLVSLMLLAGVAGQVSGGILADIYGKKEFVILGL
                     VFSIPAFFAFLATDGLPSIAALLVFGFALWSSFALTVAMAHEMMPDHVGLTSGLMLGV
                     AVGAGGLGVAATGMIADRISLDAALSTIPILVIGSLLLMLLVRYPWKSLSLPGA"
     gene            complement(40779..>41198)
                     /locus_tag="PKM87_02490"
     CDS             complement(40779..>41198)
                     /locus_tag="PKM87_02490"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HNQ28964.1"
                     /translation="VYRSRVFDEPVDGIIYAAAAGLGFATIENILYLFSAFDESLLLA
                     LQTGVVRGLISVPGHVLFSVMWGSALGMARFMPEQEGSALVRNGLVLAMAAHSLFNLL
                     LFSAVGFALLVLVLVPALWMLTERHIRHAVAHSLFKK"
BASE COUNT         9207 a        11662 c        11252 g         9077 t
ORIGIN      
        1 aaaaacgtcc aggaaaagga catgggtgcg gtgaagggga ccaatctccg tgacctctgc
       61 gaactggtgg gagggatgtc cccgggagac acagtgaagg tcagggctgc agacggtttc
      121 tccagggaat ttgcatacca gaacgtgtat tccttccctc caagacaggg gccacttgtc
      181 gtgacctggt ggaaggctga tgccggatac gttccggatt accgggaagg gatgcggctc
      241 gtattctttg ctgacaccag cacgaatccc tggggtatcc atgcctttgg aaactgggac
      301 tggcacgagt cggctgatga agagcactgg tattattacc gccaggggga ccagaaatac
      361 ccgaccacga caggcctctc ggtccagtat atttcagaca ttttgatcta tcccggcagc
      421 gctgcacagg cagaacctcg tcgcatggca atacctaacg caccggaggc tccgacacag
      481 gcaggattct cactccatgt gatcatgggt gctctcacag ccggcgttct ccttgtatac
      541 gccactaaaa agaggtaaaa gcgatggatt taagttcttt ttcccttcgt atttttgaca
      601 cacaacgaaa tcacaggatc ggattgttcc cgtgcatcat cgccgcgatc ctgctcttcg
      661 ggctgataca cgtcacgtgc gcagctccca ctacagaagt gcgtgccgcg aaatatgccg
      721 cagacggcac tacggttctg aacgagacga aggtgtcgta ccagtggatg gaagcaaatc
      781 tccaggtata tggtgacggc ctgacgcact acttccacca gggtccggtc tttgtgagcg
      841 acaaggaagg gcagtgggac gtgaacgaga ctacgaattt caaggacatg ggggccgtca
      901 gaggcacttc ggtaagagac ctctgcgacc tcgtgggggg aatgaatcca ggcgacgaag
      961 tgatggtgaa agcttccgat ggctatcacg tggagttccc ttacgaaaac atctaccacc
     1021 cacttccccg ccaggggtca gtcgttgtct gctggatcaa cggtgaggag accgcggtgg
     1081 gtgagcgcca ggggacggga tacccgcctt cctaccatgt cggtatgcgg ctggttttct
     1141 ttgctgacaa ctcgacaaat catgagggga agaatgtctt tgggaacacg gacatgaggg
     1201 aaacgatgcc tccagattcg gtccatctct ttgacaatct ctatccttcg acaagcggct
     1261 ataccgtcaa gtgggttgac gaggtgcgga tttatagtgg aggatatcac gggtcctccg
     1321 aattactccc gaaatcctac gagtcgaaga tggatgagac ataccctcct ccacccacca
     1381 ggagcccgtt gctcttgggt attcccctcg tggtatgtct tgcttgtgca ctcatgcttg
     1441 tgaaaagaaa ggatcgtgaa tcatgaaact cactatccca gttctcctca tctcatttct
     1501 cgctgtcgga ttcctgatgg ctgcaggatg ttccgacaca aagagcccgg tggccgaggc
     1561 cgctcctgac tattctgact ggcggctcac cctgaaagga gccagtgaga tggttttcac
     1621 actggatgaa ttacgtaatc tgccttcggt gaacggtcat ggatactgcg tctcgactac
     1681 gggattcagg ttcggaccct accgctgcaa gggggtagac cttcgtatcc ttgcatccat
     1741 ggctgggggt atggaacctg gtgacaccat ctggatatca gctccggacg gatatctctg
     1801 ggtttttgac ctggaccaga tggaaggcag aggcttcgtc accttcaacg agggccttgc
     1861 agaaatccct tcgccgcctc ttcgcatcat cctgatgtat gaacaggatg gaaagccact
     1921 ctcctacaat gacggcggtc ccgcacgcat tgccatcatc tccgaagaac cgggagtaat
     1981 cactgaagga agcgcgtggg tgaaatgggt agaccggatc gagattaagg agaagacggc
     2041 atgaacaggg cgatcgttcc ggtgattctc atgctcattt tctgtaccct ctactccggg
     2101 tgcagtacgc acccgaacac cggaaagatc cccctcaagg ttgttgctgc gggaagtctc
     2161 ctcgttcctt tctcggaatt ggaatcccaa tacgaggcac tgaacccgga cattgacgtc
     2221 caggttgaag gtcacggaag catacaggca attcgccagg tcacggacct ctaccggaaa
     2281 ttcgacgtgg tcgcagttgc agatgaatca ctcattcctg atttgatgta tcgcccttcc
     2341 cgggagaccg ggaagaactt taccgactgg tatgtccctt tcgcccggaa cgagatggtg
     2401 atcgcgtata ccgaaaaaag cctgtatcat acagagataa atgagacaaa ctggccggaa
     2461 atccttgcaa agccggacgt cagggttggt ttctcaaatc ccatgctcga tgctgccgga
     2521 taccgggccc tcatggtcac catgctcgcg ggagactatt acggtgacca gacaatattc
     2581 ccgagggtga tcgggaatca ttttgaccca ccccttgtaa tggaaaattc agcgcaaggt
     2641 tacacggtcc gtctcccgga ggtcccccat ccatcagacc agcacatcgt catccgtgac
     2701 gggagcatct ttctcctgtc tctccttgcc gccggaggaa ttgactatgc attcgaatac
     2761 aggagtgtcg cagaagccca gaacttatcg tatgtcaacc ttccgccaga gatcaacctt
     2821 gcctccactg attacgaaga ctggtacgca aaatcccatg tgatacttgg ctttccccgc
     2881 ttctcctcaa tcggccagga acggggcgga agaccgattg tctatgccat gacagttcca
     2941 tcagacgcaa cacacccgga tgaaggacag aaattcctgc aatttgtact gggtcatgaa
     3001 aagataggag agagaggctg gccggcctct ctctaggcaa ttgtctccat aatacctatg
     3061 caagaaataa ccggaaattt tctgatgcaa ggcagaaaag gagaggaaat ctcaacccgc
     3121 atgccgggat atgagggatc aatggggacc ccagctgcgg cgcatgttac agggagatct
     3181 ctcgcggcag cagttttttt cctgttttta tctcaagggc ctctgccctt ccctagagag
     3241 gagtgtggat gattctaccc acaggaaaag gtcgcattac tccagggaga cgacaaattg
     3301 agcggatctc ccgtatttcg agaatcattt ttgctgtatt cctgttatcg gctcttgcat
     3361 ggacttctgg tgccgttcct gaagattttg gccataataa cgctttcccg gtcaataatc
     3421 acaccatgca ggacgtaata cgatggggac cctatatcac tggtacaaca tccaatcaca
     3481 cgaatattca tctctggacc tctgaccctg tccttccgac cattcaatac gcaaatgata
     3541 gtttcaggag cgatttgttg cagcctcatc gcacagcgtt ttttaccgat ggctatatcc
     3601 accacacatt ccttctggag aatctcgctg atgcaacacg ctatgagtac cgcatctgga
     3661 gcggagagac cgagtacggt atttatcact ttctcaccta tccgtgttcg ggaccggtca
     3721 cgttcgtggt ctatggggac acgagggacg agcttccccg catctcacag gaggaacggc
     3781 accgtccggt tgcggatgtt attgccgcgg aacccggcgt ttcactggtc gtgcatacag
     3841 gcgaccttgt gaacaatgga gaaaatatgt cggactggga ccggttcttt gccaccggtg
     3901 cggtgatgtt tgcgaatgtc acccttgtcc cggtgatggg gaaccatgag aggaacagcg
     3961 atctgtattt tgaaatattc cggacttccc cgaattacac cgttgaggcc ggcaatatcc
     4021 tgatcgtggt cctcgattcc aacgactggg catggaatga cctgcctgca cagtctgcat
     4081 ggatgaatga atcattctcg cgggatgtcc cctggaaatt cgtcgtcctt caccatccaa
     4141 tctatagttc tgaagagaaa cacccagggg gctttcagaa cctccggaga gaatgggaac
     4201 cggtctttcg ggcgtgtggg gttattgccg tcttccagag ccatgttcac gtctacgaaa
     4261 gggacgttgc acatggaatc acgtatatta ccgaagcaag aggaggagct ccattttaca
     4321 cacttgccga acagaagata cccgaatacc gggtgagctc ggagaatact ctcggataca
     4381 ctgtcgtgcg gacagaaaac aattcggatt tcgtgcacat gagggtaatg gccgtctccc
     4441 ctgcaggagg gaagggagaa tccagggcca gtatcattga agagatcact cttcctgaac
     4501 cggtggaatg ggcggatgat gacagcgttg acaaaaacca gcgttcactg ttccctgcca
     4561 ggctgcagac cctgcagacc tgcagggatg ccctcttcgc agggtcggga gaggcatgtt
     4621 caggggcaga ggagtgcagg aatctcatat cttccctctt caaccccgaa gaagaggatg
     4681 gccacctgtt gcagacccgg gatatacccg agaggaaact atgacgagaa attctctcat
     4741 tacattccat gcgaatctcc atccgctcct gctcttactt atgatctcat gcgtgctggt
     4801 aggtgcatcc ggagttacat ctgctgcgac aaccgagatt cgtgtagtaa aatatgcagc
     4861 cgacgagttc accatactta ccgaaaagac cgttgactat cgatggatgg agaaaaacct
     4921 ccgtgtatac ggagatggat tcacccatta ctaccaccag ggaccagtct ttgtggacga
     4981 tccggaccct gtgaaggagg aggaactgag atggaacccg gatgaagata ccaatgtcca
     5041 ggagaaggac atgggtgccg tcaaaggaac gagtgtaaaa gacctctgtg acctggtagg
     5101 ggggatgggg tctgaagaca gggtaaaaat acgttccgct gacggctggt ccatgctctt
     5161 tgcgtataaa aacgtttacg aatattccag tcgcgaaggc cccatcgtga tctgttggtc
     5221 taagaatggg atgtttcccg atacaggata ctcggatggt atgcggatgg tgtggtttgc
     5281 cgacacctcg actaatccct gggggatcca tgcctttggg aattacgatt ggcacgaagc
     5341 ggctgattcg gaatactggt atttttacca ttcgggagac gagagatatc ccaccaccac
     5401 tggcttatca gggcagatcg tctctgaaat cctgatatac agccaggaag agcccgctgg
     5461 cagcatcgaa gtcacatcaa cccctgcggg tgccgagata gtgctcgacg gagaagagac
     5521 cggagagatc actcctttca ctcttgtcga cattcctgcc ggcagtcaca ctgtgagtgt
     5581 ccaaaaggat ggttatctcg atcccattga agagtgggtc gatgtatctc acggggttgt
     5641 cgttccggtc catttcaacc tggaacggga atattccgtc acatcgtcat ccggggatct
     5701 ggcatccacc caggacggcg ggtatgtgtc cgaacagatc tcattcggct tggtcgcaac
     5761 tgcgataacc ggcaatatat ccctccatgt tgcacctgga gagcgccaga tctgcaggag
     5821 cggacagtcg attacgtacc gcattccacc agattctttg ccgccgagtt ctttcgcatg
     5881 ggccaggctc tatctgttct ctgatgcggg gtatacccac gaacagagga ttgaggtcgg
     5941 aatcaacggg atgagccccg gcactccgga gcgttccaca tttcgcctcc ctgacggcaa
     6001 agtcactgaa acgctggcct ttaatgttac gcgtatgctg aggaccgatg ccccgatgga
     6061 gatatctgcc aggaatcccc ccggcgggaa tgaatggact ctctatcctc cccttcttct
     6121 tgttgtggcc ggagacgatg gtgacgtcca tcgcgagaac tggatttcgg aaggagcggg
     6181 ccttgtccac gccatgaagg agtattccta tcaggacctc ccgaacctca ctgtttcaga
     6241 tttcccggat atccggacag gtctttccgg agcagaactc caggcagtca gtacgatccc
     6301 ggacgatggt ggggcatcct ctcccatggc attccagaac tcggttgtca taccgggtga
     6361 atacgcgcac aaccatgctg gaatatgggt ctcacgttac aatgtcaccg gaactgtctc
     6421 ggagtataca cataatgaat tgttctggaa aacaacaccg ggaagagatc cggctgtaat
     6481 cgggccaagg atagtcatcc ttaccgtttc gtatccaccg ggactttcaa caactgatta
     6541 tacgtcaaag aatcgttcgg caatcggacc tatcgatact ctttcccctg tctctgaaag
     6601 ccccccgcca tctccgtcca cctctgctgt cacgaccatt actcccgatt taatcgggaa
     6661 tggacgtgaa gaagcgataa gggacgactc cttcttctcc cgcatatggg aggtactatt
     6721 ctggctactt gggatgccaa tacccggaca cggggaccag ggccaggcgc acgttaccgg
     6781 ggacagccca aaaggggact tgccgaatgt tgaatctccc agccatgccc cggattcttc
     6841 ttccggttac cctctccagg tcagcacgac ccccccgggg gccctcgtct cgattcgtgg
     6901 aggatcagat gtgcaaattt cgcctgccac ctttgtactg ccagggggcg aataccgcct
     6961 cgcggccgag atggacggct atctctcctg ccagacaatg gtccgtctcg ccggtccaca
     7021 ggagataacc cttgtcctga ccccggaagg aggaacagat gaaagcctac ccggagaggc
     7081 accggaaagg tcccgccatg gcggcctcct gatcgtcacc tacccggggg atctggaatt
     7141 atcggtggat aatcaacaaa tggagagaaa aagtcccctt atgctctatg gactgaaaga
     7201 gggataccat accgtgaagg caacccggcc gagtgcgtcg gccggcaagg gcgagacgct
     7261 cattacaagg gcttgggtat atcacgatgc actggccatc tgtgaactgg atttcgttgt
     7321 tgccagactg gatcggagga tacggatcac ggacccttca ggaaattcca ctgcattcac
     7381 gctcaacggg atatatccac tgctgcggac tccggttacc cttgaagtcc cgggaaccgg
     7441 gtcatatatc accctcatcg gcgaatccac ctacacgaac atccctatcc aggacggcct
     7501 tgcagacggt agcgaacttc tcctccccac atataacgga aattatcatg ccatctcaat
     7561 tgagtccgcc ccttccggtg cagagatttt tatggacggg gcaaggaccg ggaacaccac
     7621 tccttctctc gtgagggggt tatctgaggg gacacacctc gtaatggtat cattgccggg
     7681 tcacctgccg acgcacagag tgatcagcat tcccaggaca caggaggaat atatcaaagg
     7741 gactgtttca tttatcctgg aaacatatcc atccggccct ctccgggtcg agagtgtttc
     7801 cgctggtgct gcaatcttca tcgacgggat tgcaacaggt gagaagacac cgttctcatt
     7861 caccgggatt cctatcggga cccacgaact gacactccgc tcaggagata ccacgcggtc
     7921 ccgggatatc atcgtccgtc ctgacaatcc gaacaggtac attgtccatc ttctttaggg
     7981 atccctcagg tccctctcat ttaacaacag gtatatctgt tggtataacg tttgatatca
     8041 acaattgcct caagttaaat atataaagat ttagttttaa gataacattg ttcctgaccg
     8101 ggttattcac ccgctcgggg agtcgtgcga ctcgataaaa ctcctggtga ggcaaggtgg
     8161 cgtgaatact atgcgttcca agtatgcact tactctatgt tcctttcttt tgattgccct
     8221 ggtcattgtc ccggcttgcg cggcgacgac ccagctgcat gtaatacgat atgcgaatga
     8281 caacaccacg gttctcaatg agaccaccgt cacgtaccag tggcttgaag aggccctccc
     8341 tgtccttggt gatggatcca cacattatta ccaccaggga ccggtctttg tcaatgaccc
     8401 tgatcctgat atcgaagagc aactcaggtg gaatccggct gaagacacta atgaaaagga
     8461 tcagggtgcc gtcaaaggga ccaatctgaa ggatctctgc gatctcgtgg gaggaatgtc
     8521 cccaggagag accgtcgaac tcgaggcttc tgacggtttt tccaagaggt ttgcgtatga
     8581 gaatgtctat tcgtatccca ttcgccaggg gcccatggtc attacatggt tcaaggatgg
     8641 gacctatccc gacacgggct attccgacgg tatgaaactc gtattcttcg ccgatgactc
     8701 tgtcaacccg tggggatacc acatgatggg gaattatgac tggcatgagt cggcagcacc
     8761 cgaatactgg tactattaca caagtggatc ggaacaatac cccacgacaa caggtttatc
     8821 ggtaaaatac atttctgaca tcaagatata cagccaggaa gcgcctcctg tccctgtcga
     8881 taccctcttt gatggcaccg ttactctcat tgagggtgag accttcaccg tcaccgccta
     8941 caacaatgcc agcgggaact acacggtgga ctataccacg ccactcggtg ccctcgagac
     9001 cgcatccaag gccggatcag ggttcaccta cgacataacc gacaagaact acgcctcatc
     9061 aggagcactg ctcctggata atattggctc gtatatttac cagaagacgc ccaggaaagc
     9121 ctggtatgcc tatgtcaatg atgtatacaa ggacgggtat aacaatccgg ccagtgccct
     9181 gaacctgata ccgctgaatg atggcgacac agtcgagtat tacttcgttg acggcactgt
     9241 ggccgatcca actgattatg ctgcagtgac cgcagcagcg accgctgcgg taaagatcat
     9301 tgcggatatc cagcctgcag gaccagttgt ggacaccctc tttgatggca ctgtcacgct
     9361 tactccggac accacgttca ctgtcaccgc ctataacaac gcgagcggga actatacagt
     9421 ggactatacc acgccactcg gtgccctcga gaccgcatcc aaggccggat cagggttcac
     9481 ctacgacata accgacaaga actacgcctc atcaggagca ctgctcctgg ataatattgg
     9541 ctcgtatatt taccagaaga cgcccaggaa agcctggtat gcctatgtca atgatgtata
     9601 caaagatgga tacaataacc cggccggtgc cctgaacctg atagcgctga atgatggcga
     9661 cacagtcgag ttctacttcg ttgacggcac cgtggccgat ccgactgatt atgccgcagt
     9721 gaccgcagca gcgaccgctg cggtgaagat tacagtcgac atcagcgggt ccacacccac
     9781 ggaaccggac tgggaactca ctctcactgg tgcccgtgac cagacagtta caaagactta
     9841 tttcgagcag ggacttgcat gcccatcgtc cggtcacatg gtgagctgga ccgatgcgga
     9901 aggggacgag tggagtggag ttccattctg gctcctcgtg gcaatggtcg atgacgatcc
     9961 agactccggc cctgaccatt acaacttcaa tgatgcgctt gctgcagagg gttactcgat
    10021 aaaggtgacc gcggatgacg ggtattccgt caactttgag agtgcagaca ttgcgcgcag
    10081 cgatgattac atcgtggcaa acgccctgaa cggcgaaccg ttgccactgc tccgtcccaa
    10141 cagcacaaag ccctgcttcc cgctccagtt gatcgggccc gcaatcattt cagggaagat
    10201 gattgggaac atctcctcta tcgaattgat tggtctccca aaacctccgg agggatggac
    10261 actgacgatg gagggtgacg tggttgatgc gatcagccag gcctacttcg aagaggccat
    10321 ggactgcatc caccctgcga gctggaccga tgggacaggg aatgtctgga caggaattcc
    10381 actctgggaa attgtcggat ctgtcgatga catcgagact gcgccacact ggaccttcaa
    10441 tgatgaccgt gccgccgccg gctacacaat cagggttatc gctggggacg ggtataacag
    10501 gacctttgca agcggtgacg tggcacgaag cggcaactac ctggtagcaa acacaaagaa
    10561 cggcgcacct ctcgtcggga ctgaagctcc gctccgcctg gtgggaacaa atgccacggg
    10621 tggaaagagc gtcgggaaca ttgctacaat caggctggaa ggcctccctg cataccctgc
    10681 cggcaactgg agcctgcttc tcgacggtgc tatccgcgac acaattccgg aacccgagtt
    10741 cgaagactgg gccgcctgcc attccgcgac gtatgacgac gggacaggga acgtgtacga
    10801 aggcatcccg ctctggcggc tgatgggctg ggttgacgac cggattcccc acgggccgaa
    10861 cgggttcaac aatgcgctcg cttctgccgg atacaaggtg atcgtgactg ccggggatgg
    10921 atacagcaag gagttcacga gccaggagat aggaacgacg aacggcttca tcatcgcgaa
    10981 caggataaac gggcaaacgc ttcccactga cggagcgcat ccaccgtatc cgctccgcct
    11041 cgtgggtgca ggcctcccct cggtccatta cagcgttggc aacgtcgtca agatcgagct
    11101 ggccgacttc caggaaccaa cagagatccc ccagatccac atcatcaaat atgatgctga
    11161 cggcgtaaca atcatcaacc agaccaccct cgaccatatc tggatggagg cgaacctccc
    11221 ggtcatcggg gacggggtca cacatttcaa gtaccaggga gtcacgtttg atcctggcga
    11281 cctctgggac ccaaccgaga ccaaggggat gaaccctccc aagatcgata acgcgatcaa
    11341 gggaacaaaa gtcagggatc tctgtgacct ggtgggcgga atggagcctg gcaccgaggt
    11401 tacctttgtc gccaccgatg ggtgggagac gacacttcca tacgactgca tctacaccaa
    11461 tccgcatgtc tattcccacc ttggtgagac gataattgcc tggtatgctg acgggcagta
    11521 tgtcccccaa tatggtgacg ggccgcgcct cttctttact ccggaagacc atgttgcagg
    11581 gcaatggaac atgcacgaag ccctggccca gcaatactgg cactactact gggacggggg
    11641 gatccagtat ccctcggtcg ccggtctctc ggccaagtac gttgacacca tcaagatcta
    11701 cgcttccccg gtaacggact ggacgctcat ccttgacgga gaggctatcg aaggcctgaa
    11761 ccagacgatc tcccgcaatt actttgaatc tgcactcgcc tgccagttcg gtgcggacca
    11821 caaggcttcc tacacggact cgcagggacg gaactgggaa gggatgccgc tgtggttcct
    11881 ctgcggcttc gtggacgatc ccgaccagca ctctgataat gcatataacg atacaaaggc
    11941 cctcgatggg taccggatca tcgtcacagg gtcagacggg tacaacgcta cgttcaacag
    12001 ccaggacacg atccgcaacg ccaactttat cgttgcgaac accctgaacg gaacccgcat
    12061 cgcggagggc gattccagct ggcctctcag gctggttggc cagaacgtga caggtggaaa
    12121 ggtcgtaagg agtgttgcgt cgatcgtgct cgcacccctt gcagaacctc ctgttgcaga
    12181 ttttgaggcc tcccccctca gtgggaatgc accgctcctg gtccagttca ccgatctctc
    12241 cacaggcaac cccctcgaat gggcctggga cttccaggat gacgggacca tcgacagcac
    12301 cgcccagaac ccgtcctgcc tctaccagga cccggggaca tactcgatcc ggctgaccgt
    12361 cacaaatgca ggagggacca gtacaaaaac aaagagccag tatgtcaccg tgatcgtcgg
    12421 gccacccatc gcggactttt cagcctcacc cgtgacaggt ccttcgccgc tccaggttca
    12481 attcaccgat ctctccacag gcaaccccct cgaatgggca tgggacttcc aggatgacgg
    12541 gaccatcgac agcaccgccc agaacccgtc ctgcctctac caggacccgg gaacatactc
    12601 ggtccggctg accgtcacaa atgcaggagg gaccagcaca aaaacaaaga gccagtatgt
    12661 caccgtgacc gtcgggccac ccatcgcttc gttcaccgcc agtccccgga gcggaaactg
    12721 gccactcacg gtcagtttca ctgatacatc cactggtagc cagatccaat catgggagtg
    12781 ggactttgga gacgggaaga agagtcccaa gaggaatccc attcacacgt atctgaaacc
    12841 aggggtctac accgccaccc tcacgatcag gtacccggag ggatctgata acgcttcgga
    12901 gacaattgaa gtgaagggtg atttcaccgc cagtgcagtg ataaaaactc ctgtcgcgtt
    12961 gttcagccag agttcggtca ttggcactcc tccactctcg gtctcgttta ccgacctctc
    13021 gttgaaaaat cccaccacct ggttgtggga ctttggagac ggtggcgctt ccaacgaaca
    13081 gaacccgtca catacatttg ataaggctgg gatgtacacc gtcaggctga acgtgaccaa
    13141 cgattacggg ttcagcagcg cagtaaaacg tgtctttgtg acacccatcc tctctttctt
    13201 ctccagataa ttttttttcc ccttatctcc ggacctgatt tccagtttga tttcaggaaa
    13261 tgcgtttctt cacatagcga atgccatacg cgaggcagat ccctgccgca aatgcagcag
    13321 tcatgtttac aaggacagag aggaccgcaa taagaccggt aacgaggtaa gagtccgtct
    13381 tgattccgtg gcggccgagc tcgatcgcca cgaagacgag cagggccgcg tagaaacccc
    13441 ctgaaacaag tgaaaggaca tcggggctcg caaagaggag cgccagcatg aggaaaaaga
    13501 tacccgcgta gatattcgca cctcccgtcc gcgccccgaa ccggtactgt cccgccatcc
    13561 cgcccgcgcc atggcacatg ggaaaacccc cgaaggggac cgtggtcagg ttcatcaggc
    13621 cgatagtcct cgatagccga gcaggccgta cttcctgtcc aaagaggtca cgggtcagca
    13681 gtgatgttgc cagtatcgcg tttgtgatgg tgaggagcgc ctgaggaacg acaagcgtac
    13741 caagtgccgc agggatgtca ctcatggagg ggatgacaag gtgtggggga ggaagaagtg
    13801 ccatcgcagg gaacccatgg aggatcacgc cggttccgaa tgccactccg atgagtatga
    13861 cggcagagag gtcaggaatt tcagtaaacc ttgcgatcac gtagactgct gccatgagaa
    13921 gcacggcaag cccaaatacg aggaggtcgc ctgtcatgaa ccctgtcgaa gtttttacaa
    13981 gaaggagcgc aaggccgagc tggatgcccc ggatcacgct ctgcgggata tacttctcca
    14041 gcgcgtcgag ccattttccg tatccgagca cgaagaagag gagacccagt ataagacctg
    14101 ccgcggcgat ctggcctgcg ctcatgttct ctgcaatggc gaccaccgcg atcactttca
    14161 tcggttcgat ggggacgggt atctggtaat agtaaccggt aatgatgaac cagatgccaa
    14221 aaaagaggag gatgtacccc agagagagcc ccgctactgc cgagactgca agtataaggg
    14281 gaagtatggt gccaaagtcc ccgacacttc cggcaagttc gcggatatta aatgaaatat
    14341 tccgggtcat ttccacccgt cccccgggaa agagggggat atccttctag cgggccctga
    14401 tgatggtccc tggcatgcca cctgaaagaa ccttcctgac attccctggt gtgtgaccgt
    14461 tcacgatctt cacttcgtga acgttcttcg catacaggag gagttcgacc accttcttct
    14521 caaggaccat atcatcaaga ttcatgtcaa ggagctctga tgcggtaatg tcaggaatac
    14581 gctcggcatc ctcgtggagg aagggattgg cagtatacag tccgtccacg ttctttacaa
    14641 ggacaaggtt cttcgcgccg agcacttcag cggcaaggaa tgcaccggta tctgtccggt
    14701 gaggagggat ggtcccgatc tcagccggct gttcgtagag accgtaggga ggagtcccat
    14761 gagttacggg gaggactccc aggtgaaaga gcatcggaag ctccaagaga tccgctgtat
    14821 gaatacgcat cccattccag cgcgagaaga ggagggacat catgatggcg ttctgctcac
    14881 ttatctttcc cgcgagttcc gcaaggaccc cggtgggcat cccgagatcc aggcccaggt
    14941 caaggatgtg acggaccctt acccccccac cggtaaccac caagattttg tgctcttttg
    15001 agagatcccc gatctcctct accaggggca ggaccacctc tttgccatag tccatgatcc
    15061 catgtcctcc tatctttacc acattcaggt ctggaaccag gcggatttgc tccccaccct
    15121 cgaaagagcg aagcaggctc tctctcacca gggactcccc ctgcagcctg ttcgccactt
    15181 caaatcgttc aactctcttt ctgtgcacga tagttttata tgatggcatg agtaatacaa
    15241 tctatgtaaa ccaactggta tatcacgtgt tatgcttaac actatatacc cgggacgtga
    15301 caagatatca actggagtga gtgaaatgat caaaatttat gttcgtgagc gccagaaagt
    15361 gcgcgagggt gcgaagcagc cgatgttccg gatcgttgca gtgacgggcg ggcaggtaca
    15421 gatcgaactg acgcatttcc gcaagttcga gattgagcag attgcaaagg acatcggggc
    15481 agaggtggta tacctgcagg agatccccga cgaagagaag aagaaagcct gaaattcacc
    15541 cccttcggat ggagggaacg gttcaatttt tttctccaaa cgctcatgaa tttaatttat
    15601 attgtttccg tgcgtataac tgccctgact ggatgccatg gctgcgttaa gtatcgacct
    15661 catattcact gcggcgatcc gaattttcct tgaagaggag aaggcgatta ccatcagtta
    15721 ttcacccgga gaagtccgga tcaggtttcc caccaccagg aggcttgccg agtttcttga
    15781 ggtcccacat tactatgttc tcccctactt tgccatgatg gaggagatgg agcttgtgac
    15841 aagggctgag agggttggaa tcctgaccac ctcaagcgga accaggaagc tcataacgat
    15901 gatgagcaaa gacttcaggc cgcaggcgtc tgccgtgatg ggagagccca tccttgccta
    15961 cctcgagtca agtgtcagct cgcaactggt atagtaactg atataccact agatacgccc
    16021 tttttcaaaa ttgagccctt ttatcaggaa tattgataaa tcttgtcgtg aaagagaatc
    16081 gttgatgtga tgctgggtat gtatacccag tcacggtcac gcaaggggca tgcatgaacg
    16141 agatcatcga aggtttcgtc caggcaggtg agctgatcat acgcctggac cccgaagtcc
    16201 tggaaatcac tgcgagatcc ctccttatct ctctctctgc gacactcatt gcgtcactca
    16261 tcgcgattcc catcggcggg ttcatccatt tcagggagtt cgggggaaag cgcggcctca
    16321 tcaccatcat ccagacactc tatgcacttc cgaccgtcat tgccggactc ttcgtattcc
    16381 ttctcctctc cagggtgggg cctttcggat tcctcggtct cctcttcact cctacgggga
    16441 tgatcatcgg ccagacagtg cttgtcatcc cgctgatggt gggaatgacc ctcatcgcac
    16501 tctccggggt gaaaaagacc atccaggacg aggtgatctc tcttggggcc acctcctccc
    16561 agatcatctg gacgattgtg aaggaggcac ggtttgccat cctcggtgga gtgatactgg
    16621 gattcggccg cgcgatctcg gaggtcggcg ccgccatgat catcggcggg aatatcaggg
    16681 gatacacccg gatactcacc accgcgattg cacttgagac ctccatggga aacattgcct
    16741 tctcgatcgc acttggaatc atcctcctta tcatcgccct tgtggtcacc accgcactct
    16801 tcctggttca ggagcggtaa gatgatcgag tttgttcatg tcgacaagag gtttggggag
    16861 aaagtcgtcc tcaaagacct tacgttcagg atcgaaaagg gagagatctt caccttcatc
    16921 ggtccgagcg gcacaggcaa gaccaccatt ctccgcctga tcgacctgct ggaaaacccc
    16981 tcgtcaggat cggtcctcat cgaaggaaac gatacaaaag tttccgaagg ggaacgcgtt
    17041 gcacttcgga ggcggatgag cgtcgtcttc cagaaacctg cagcactccg cgggagtgtc
    17101 tacgagaacg tggccctcgg gctgaaattc cggggcaccc ccgctgacga gatgcgcgtg
    17161 agagtcacgg aagcgctcgg cctcgtaggc ctcgatggct acgaagagag gaaagcgacc
    17221 accctctcgg gaggtgagct ccagcgggtg gccatcgcac gcgcacttgt cagcaggccg
    17281 gagatcctcc tgctggacga gcccaccgca aacctggacc cggcatccac agaacgcatc
    17341 gagaacctga tctcttccat aaacgagcgg ttcgggacga cgattgtcct ctccacccac
    17401 gacatgatcc agggccagcg gctggcgaca cgcatagcag tgatactgaa ccggaccatc
    17461 ggacaggtgg gggactccca ccagatcttt taccagccga aaagcaagga ggttgcccgg
    17521 atggtcggtg tcgagaatat ctttgatggt gttatcgctg agaatacagg tggcctggcc
    17581 acgatcgaca ttggaaacac gtgtattgtg gccatatgcc cctatcctga agggacacgt
    17641 gtcacctcgt atatgcgccc tgaagatgtc attttcctca ttgagaagga tggaaaaagc
    17701 accgcgcgca acgagatcga gggaaccatc tctcgtgtga ctcccatggg gccgagcgtg
    17761 aaggtcagga tggatggcgg catcagggtt gttgcagtca tcacccgtcg ttcatacgat
    17821 gagcttggcc tctcgccagg cgcccatgtc tatgcctcct tcaaggccag cgccatccat
    17881 gtagtgaaga acgaaccgta aatccgatgg cttgccggaa aaggttggtg ttcaggcagc
    17941 ctcactgaac accagtcata cccgctaccc tgcataactc tgacccttca tatctaccgg
    18001 atctccttct gcctccctag tggaacgcac ctcattgcat tgaccggcac ccatttctcg
    18061 catgcccgcg tatacatgat gcaatgcggt gcatcgccct tgcaagcggc agcaaaggga
    18121 actgcctcta tatcgaaggg agttcatcct cgctcctcat tgatgcaggg ctctccaggc
    18181 gcgagatcct ctcccgcctg cattccgtag gcggatccac ggagcatctc tcggcgatcc
    18241 tcgtgacaca cgagcatatc gaccatatcg catcagtaga ggcacttgcc cgccacctct
    18301 ccatcccggt ttatgcgacc ggcgggaccc tccttgatct ccttgattca cggcctgtcc
    18361 ggagaaatcc catcagggcg gtgcgggtcg attacgggga gcggtttact atcggggatt
    18421 tctctgtgga ggccttcgag gtctcccacg atgcgaggga accctgcggg ttcctggtca
    18481 ccgaagggga tctcacggtt gggtgctgca ctgacaccgg gatggtgaat gaccatatga
    18541 tggactgctt ctgccggtgc gatgcactcg tcctcgagag caaccactgc cccgagatgc
    18601 tgaagaacgg gccatacccc gaggtgctga agcgtcggat ccgttcacga agggggcacc
    18661 tctccaaccc cgatgccgcc cgctgcgtga acgaactctg cacgcgggtg cacatgatca
    18721 tgctcgcgca cttgagcgag atgaacaaca ccccggaaaa ggcactttcc acggccaggc
    18781 agggagccgg gctcttctca accgacatcg agatcaccgt tgccacgaat cccggcaccg
    18841 ggcagggctg gccccacggc atcagattgt gaatttttcc tggaagaatt caaaaactgc
    18901 ccggaggacc gattctcccc ccttcacatg agagcgtaac gatacctacg caggcatatg
    18961 ttgagccaaa atatccgttt ggtttctttg tttccttcca tgggaattct gaactcccgc
    19021 gccatggagg aatgggcgta atgccctgct ctgaaaggga atgagagtga tttttttggt
    19081 gcgacatcat ggttttaaaa aaaagtcttt ttcttggccc tttttccgaa tccggggaga
    19141 aattcttcat ccttccttca tcttcaacag cttgaggctg tacatgtcga ttgaatcaag
    19201 gaacagcttc tttgcagtcc tggagcgttt ctcatcctct gaaaagatgt cccccatgag
    19261 gtattcgagg acccccaggt aatacccgag tgccgggagc ttcttcttgg tatcttcaag
    19321 catcacctcc gcatcggcct tcctgccggt gatgatcatg aacgagaggt ccagcatgag
    19381 gatgatgtgg gccggcatct tttttgagat cgcggtgctc cggaacttct ggtacgcaga
    19441 ctcctgggat gagatgatgg agagctgcag gccgtaatag atcggctcga gctcgtctgg
    19501 atgctgctgc atcatctgcc ttacaacagc ggtcgcgccc ccggcatctc tcccctcgag
    19561 cttgaagtga tacaggtcgc ggaggaagaa catgtcgtcg tagtaccgtt ccgatgcaat
    19621 cttcaggagc tcgcccctca tctccctgtt tccatccttc ttcgcctttt cgaggccgat
    19681 cttcaggtac tccggctcgt ttgggaacca gtcgatggca agcctgatca tgaaccagaa
    19741 gaccttgttc acgcgtagct cctgcacaac ctgcgggaac ctgaggcggt tgaccggcgg
    19801 ccggagccgg gcaagctcga tcatctgctc ccgtgcggat agcgccatgg cactcgtagt
    19861 ggccttgtcc ggtttcttgt ccggagggag ctcgagggag gagagttcgt aataggagaa
    19921 ggcgatcgcg gtgcatacca tcgcatcgac cgttcggtac ttcttgagca cctcgattgc
    19981 atccgagtac ttccccatgt cgatcagttt tattccaagg acgatgtcga agattgccct
    20041 ccccgtgtac ttcctcttga cacgggttgc gtgtcggaaa agcttttcga tgtactcgat
    20101 gtcggtggaa tgcctgctct cctcgaggac ttttacggtc acgtcgtcga tgaaacggtc
    20161 gtatgcctgg tcggggatgt cagggtagtt cttctcgagg atcgagacca cgtggccaaa
    20221 gaatatcggg atgttcgagt cgatcttctc agagttcttc tctacgtact ccatcatctc
    20281 ccgcttcgtc tgccccatct tctgctcgac gagggcatcc gggatccact cctccggcat
    20341 tgtatacagg aatgacctgt aagaatataa gaaccttgat atactgtgcc gaaatcgggt
    20401 attcaaccgt tcagataact gacccttcgc agaagggaac ccttatcctc gcccaacgtg
    20461 actattctaa atatccaggc aatcatgaaa tttgcagaat ttgcaaggat ctgcgaggag
    20521 atcgagggta tttcagggag gctagagatg gcggacaaga tctccggcat cctcccgggt
    20581 ctcgattcca gggacttagc gatattcgtt cattttatca cagggaagat cttccctgac
    20641 tggagtccgc tcaagatggg gatcggtcca aacctcctct atgacgcggt caccctcatc
    20701 gtcgatggaa caaggagtga cgtgatcggt gagataaacc gccgcggaga tgtcggcctt
    20761 gcggtgcagg tgatactcgg gcagaaaaag gtatcgcgaa ggtcgccgcg gactgcgacc
    20821 atccagacct ctctcttctc ccaggaactc gacctcttcg gcgtctacca gaaactcgag
    20881 aagattgcgc ggcagggtgg acgggcttca cagcaggaga aattatccct catacagagc
    20941 ctcttccaga acgcgtcgcc gcttgaggca cggtaccttg caaggctcct gatggaggac
    21001 ctgcgtatcg gtgtggggga ggggacgata cgggacgcca ttgcccgggc attctctgtt
    21061 gatccttcga acgttgagca cgcgtaccag gccaggaacg accttggcga ggtggcaata
    21121 cttgcaaggg ccggtgagaa tgcactgcgc gacgtgcgca tcctgccgtt ccacccggtg
    21181 aagatgatgc ttgcccaggc gggggatatt catggggcga ttgccgagaa cggagccatg
    21241 gccgctgagt tcaagtacga tggcagcagg ttccagttcc acaaggcggg cggcgagagc
    21301 aggatatact cgaggaaact cgaggacgtg acagacgccc ttccggatat cctttccgcg
    21361 ctttacgggg ctaccaccca tgacgtgatc cttgacggcg aagtgattgc ggtcaaagac
    21421 ggaaaacccc tcccgttcca ggccgtgctg cgaaggttcc gtcggaagca cgggatcgag
    21481 gagatgcaaa aaacggtgaa tgtgcaaccg ttcgtctttg atatcctcta cctcgacggt
    21541 gaaaccctga tcgacctccc cttcagagag cggagagcgc ggctcgaggc gacggtcagt
    21601 gactatgtca cgccccagct ggtgagtggc gaacctgatg caatcgagag attctacaaa
    21661 ggaagcctcg ctgctggcca tgaaggactg atgctgaagg tgctctctgc accgtatacc
    21721 ccgggaatac ggggaaagaa ctggctgaag atcaagccct ccgtggatac cctggacctc
    21781 gcagtcatcg gggcggaatg gggagaaggg aaaagagcgc acctctttgg ttcattcctg
    21841 ctcgcatgcc aggaccaggg ggaactccag gcactgagca aggtcgcaac cgggttctcg
    21901 gatgagcagc tcgcggagct gtatgatctc ctccgggacc acgttctctc agagtccgga
    21961 aaggaagtgc agttcgagcc aatcctggta ttcgaggtag ggtactcgga gatccaggaa
    22021 agccccaact acgagtgcgg atacgcactc cggttccctc gcttcatccg cgtccgtgaa
    22081 gacaagggta tcgcagatat cgagaccctt gacgggataa gggaacgcta catacgccag
    22141 aagaagtgat tgtgcagcag gcgcagggtc gaagaactgt tgtcgctgga ttgcccgttg
    22201 ctttgaggcc tggaaagccc cttttagtgc atgcgtcgcg cctcccggct tcaactgatc
    22261 cgactgtaac tatcaggaca acagtgggcc aggggtaata gtatcttatt tgccagggaa
    22321 ttggatccgg gtcgaaagat catggttcag gtggttttcg gatcacagcc ctgtcaccga
    22381 tatgcgcgat aattcgcgcg gtacattcct cccacgaggc ttccccctcc ggaacaatgc
    22441 ccgcgacctc cctgccgagt tcgtcgtcgg aaatgccctg ggcgaccgca agaatggagc
    22501 ccttcgcata tcgttcgggg acgatattat cgctatcacc gtcaacaatg ccgaggatgg
    22561 ggattcccag gtgcgccccg atatgtccac agacggcagt ggtatcgtct cccaccgtta
    22621 ccatcccgca gatgttccca ttgccgatca tccgataaag gtgatgccca cagtggtcga
    22681 caaatatcac ctttcccact attgcatgcc ggggtcctgc cagagggcct tttgacctga
    22741 gtctcccact cttgcaccat gcacgggaca cgtcgacggg accggccctc tccagttttt
    22801 caagcccgtg gtccttgact tggagacccg ataaggggaa aatcttctct ccctcggacc
    22861 gaatgacgac ttctctccct gttgctgtcc ctataactac cccgttgacg aacactgctt
    22921 cccctgggag acatcccctg attgtccgat atccttcatc gggcatgagg gcgggagcca
    22981 ttctctcaac caccgggtaa cctgtcagcc ccgagatttt atgcactgcc gcatcaggct
    23041 tcctgttcca gcagatgact tccctgcgtg cacattccac ctggatgagc ccttctccac
    23101 ctacgcgtga ggcgactatc tccccgaata tccgccccga ttcaggagtt ttcccctcgt
    23161 ttgcgaggaa ggaaggggcc tccagcatgc gtattgccac gcttggggga acgctcatga
    23221 attcgcatgg aagtcctgat tcctctgcgg cagctctcgc catcacccct gcaacgacaa
    23281 tctttttcgg ggcgagcacc cgagagagcc actctgcatt tcccgaatcg aagacctcag
    23341 gcccatgcag gaccatcacc atctggcgta tgtcactgtt cttcgtgcgg tctcccggag
    23401 tgtctgtcct tacctgacaa ggatgtgtcg cggtgtcgtc ttttttattc atgggatcgt
    23461 cctgtgagag aatactgggt gtcgtgtcat acgacaagtt acgcagctgc cggtgtgaat
    23521 ggtcctgaag gtatgccttt tgcaaccggt cagttgcgcg gaaatggtcc catcctcttt
    23581 atacacggtt gaaatcatca gagaaccggg tgatgtcgtc ctcttcgaga tactcgccga
    23641 tctggacctc gatcacctcg agcgggatct tgccggtatt ggcaaggcgg tgcctcgtgg
    23701 cggcaggaac gaacgtgctt tcgccctgcc ggaggtggat ggtccggtcg ccgacgatca
    23761 catcggccat tccgctcacg atgatccagt gctcgctccg gtggtgatgc atctgcaggg
    23821 agagtctctt gccgggtttt accgtcaccc gcttgatcct gaagaacctc gaacgttcaa
    23881 gctcggtata ggagccccag ggccggtgca cctggagatg gtagtcggtg atcgtatcgc
    23941 cccggtcgcg gaatctcttc acaagttcgc tgaccttctc ggtcatatcc atgtggcagg
    24001 ccaggagcgc atcaccagta tccaccagca caaggtcacg aactcctatg agcgctgccc
    24061 gtttcccggg agcatgtaca aagttacgct ctgaatcgag catctccgcg tggcctgcat
    24121 tgccgtaatc gtcgtgtggc ctgagttcat accatgcccg gaacgtccca aggtccgtcc
    24181 agcgggattt gagggggacc actgccacct tctccgaatg ctcgaggagc ccgtagtcaa
    24241 ccgagagcga gggaaggttc gcatactccc ttataccatc cctgaactgc gaatagagtt
    24301 cgggctggta ttttgccagt tcgtcgaaga agaccccggt tgagaagagg aacatcccgc
    24361 tgttccagag gaacccggcc tccaggtata cctttgcggt ctcccagtca ggtttctctc
    24421 tgaactttct tacccgggcc cctaacgcga ggggatcccc tggatcgata tacccgtacc
    24481 cggtatgagg cgttgtggga gggactccga aggtgacgag atacttgtct gatatacatc
    24541 ttgccgcggc aatctccttc atggcgccat tttcgagatt atgatcgctt ggaaagaccg
    24601 caacgacaga gtctccatat tcttccctga ttacctgcat ggcccaggtg attgccggaa
    24661 gggtattttt cgcgacaggc tctttcagga ttcgctcatc ttttacatgg accccaacct
    24721 cctcaagctg gctcctgaca aggaactggt gggcttcgtt cgtgacgacc cagacctctt
    24781 caggccctga tatcaggagc gccctccgcc aggtgtcctg aaagagcgag gtgccattca
    24841 ctttcaggaa ctgcttgggg ttatatgtgc gtgacagggg ccagaggcgg gttcctaccc
    24901 cgcccgcgag gatgacggac ttgatggatt ccattcggtt aactctcttt ggacgtgttc
    24961 gcacttatga tttacccgac cgtatcctct aaaaaatatg aaccgactcc ccccactccc
    25021 tcaatcaaga gcactccgca gacgctgtat gacatgcttt gttacctgat cccgggtatg
    25081 ggaatccatg cagaagacct ccacgtcatc ccggcatttc agcgactcga taaagggggt
    25141 ccccctgagt gcaatggtgg caagcagtac cgggaaggag tccaggagat ccaatatacg
    25201 catgcaaaag agagaagaga ggcattccat cttccctatc tcgtcaatga tcacgaatgg
    25261 tgattcatcg aagtgcaatt cttcaaggaa cgcgtcaaac gcagccacat cgaccccata
    25321 tttccccact ctccaccctg accggatact gatatccgcg agaatgccag tctttccgca
    25381 gaaagagacc atctcgaacc ccctcctctc tcccttctcc ctgacctccc tcgtgacgaa
    25441 gccttcaggc cggattggcg caaggctttg ggcaatcccc tccacgaggg ttgtctttcc
    25501 gcacgccggg gcgccggtga tgagaatatt cggcccggcc tttgcctgct tcatggattc
    25561 acccgccggt tacgcgccat tcgtgcgttt tttcatccct gcaattgcga ttgtcacgga
    25621 taaaatgaga gaaagcacgc cggtccaccc gtcaagcatt ggcgcagaat gggccgtggt
    25681 gggatctgtg ggggggattg acgcaggggg catggtggcc tgtacagtct catgctctgg
    25741 ggcaacagaa attccaatag tcggaacctc ggtcggcact ctctccatca taaatgaccg
    25801 ggcgacacgg agattctcct ctgcgagcgt gtttcccggg cggatgccaa gcgatctctc
    25861 gtaagcctca gccgcctctt gatactctcc catcacgaga taggtatttc caaggcagaa
    25921 ataggccctg tcgtggcccg ggctgatcgt caccactttc cggtaggtct cggctgcttc
    25981 agggtactgg cgcagtgtga agagaacctc cgcttttccc atcagggcag gggcattgta
    26041 gggatcgatc gcaagtgagt cattgaatgc ctggagtgcc tcaaaatagt gtccgctcat
    26101 atacaggtcc tgcgcagttg ctgccatggc agcagggtcc tgcgaagagc cataccctga
    26161 tgaactgtct ccatcagacg aatcaccggc atcgccggaa cccgaatccc cgccgcaacc
    26221 acagtcttcc gcgagcacgg gagccacgtg gagagccagg atgacaagga gagccattat
    26281 gcccagaatc ggccgccacc tcatgtacta tgaccctctg cccacccttg atttaaaggt
    26341 aatgaaaata gtaaaaaacc gctgattctg atgaccccag ggtgtactgc gggtatcgat
    26401 tgtgaaaggg gatgtctgat acctccggaa gtatcatgcc ccttgaacct gcttgagcgc
    26461 cacttcgatt gccactctca gctcctcgtc cttgaatggc ttgagtaaaa atccgctcgg
    26521 accggtctca attgccctcg agagagtctc gttatcggaa tgcgcagtca ggtaaatgaa
    26581 aggtatgcag tactctttcc tgatctgcga accaagtaca gttccatcca ccccttcctg
    26641 cagggcgata tcgataagga cgaggtcggg aaccttcgat tttaacaaag aacgtgcctt
    26701 ttccactgtc ggtgcagtcc cgctcacctc gtacccgagt ttcttcaggc gccacgatat
    26761 cagctggctg atgacgggat cgtcttcaac aacgaggatg gacttcgtca cttgtgtata
    26821 cccccctcaa tcctctcctt atcaaaggta tctcccaatc ccgctttatt aaactgatga
    26881 tcctcatgaa aatgatcagg gagcattact ccgcttcaat gacctgaacg tgcccttcgg
    26941 aaagaagacc tcgaaccgcg cccctgtccc cggctctccc gtctcgatca gagagatgcc
    27001 ggtaatatcg aggatctccc gggcaaggaa gaggccaaaa ccgctgccct tgccatagcc
    27061 cttctcaaag atcttctcct tctcttcgtg tgggatgcca gatccatcgt cttcaacgga
    27121 gaggatcagc ccatatgaac tcatggtggt actgatggat atcgcagtta tcccgtcccc
    27181 gtgcctcatc gcattatcca cgaggttatg gaacacgcgc tcaaggagag gatcggcgta
    27241 cacttccagg tcgcccacct cacagtgcat gcggattgca gagagatcga atgactgcag
    27301 tgccctctgc aggaccccat tcatgtcctg ccatgtggga gcacgcaccc cgatatcttc
    27361 gtactccttg gtgaacgcaa cctgtcgctc gatattctgc cccacgcgct gaatcttttc
    27421 gatgaagtca accagccacg gactctctgc ctgctccttt gccatgtcca ggtaaagcat
    27481 gagagcggtg acctggttga ggatgtcatg acgggtgata cgggagagga ggctgagctt
    27541 ccggttcgcc atctgcaccg cttcctctgc tttcaccaca tccgtaatgt cccgtcctac
    27601 cgactggaac tcccgtatcc cgcccttctc gtcaaagatt gcctggtcag tccagctctg
    27661 ccagcgcacc tctccacccg gcaggatgat acggtgctgg atgtatccgg tcggattttt
    27721 aggggtgaga gatgcaaaat gcttcctcac aagcggcatc tcttccgggg gtacgtgggg
    27781 acggaaacgg ctcccgacaa tctcgtcgta cgataactcg tagtatcgac aaaacgcagg
    27841 attcacgaag atatacgttc catccgggag gaaacgggca atgagatcct cctggctctc
    27901 aacaatcccc ttgtacctcg cttcgctctc cttgagcgcc tcctccatcc ggcggcgctc
    27961 tgatacgtcg cgaatcgatt cgatcgcacc gataatgttg ccttcatggt catagagcgg
    28021 ggaggctttc ccccagagga tcgctttctg gcctcccagc ctggggagat cggtctcgcc
    28081 gataaggaga tcccctttct tatgcaccac gccgtaatac cgcttgacta tctcctcgga
    28141 ggattcgaag atgagatcaa tgaggattgg gcgcctctca ctgtaaaagg ggatcgagta
    28201 ttcataattc cccttcccta aaatttcgcg ggcagggaca ccggtcatct cctcgataga
    28261 gtagttccag gcaatgaccc tgccttccct gtcgattgca aatgtcgcat ccggaaggaa
    28321 attgatgata tctgagagcc tctgttccga tctctggagg gcaagctcag cccgtttacg
    28381 gcttaccgcc tgctcgatca tattccgcag ctctgcaaac tgcgaccggg ggtgaccacc
    28441 tttctggagg tagaaggcag caccgaggtt gagggcctcg atcgcaacat gctctcttcc
    28501 gcgccccgtg aagatgataa aggggatgtg ggggtgggtt tccttgatgg cccggaggaa
    28561 ttcaatccca ttcatctgcg gcatttcgta atccgacacg atgacgtcga aaccacgtgt
    28621 cttcagcatt tcgagggctt catgagcgct tagcgcagtg gagatctcca tggccccacc
    28681 tttttcgagg aatatcttcg ttacatccag gagggatgct tcatcatcga ccaggagaac
    28741 ctcaatcagg accgatcacc ccgggggcac gcgtatttat cactccttga cctgctgaaa
    28801 gataataata acaggttctc caggataatg tacattatct catcatgatc tgcacggctg
    28861 tcgggtattt cctcaggagt gcaaatacga gcgcccgtgc cacccacatc ctgcccccct
    28921 caatacggcg ctccctcgag agatcgccga gttcctgcaa cacggcaatg gcaacttcat
    28981 ctgcaagcat gcagctgttt gaaacctcca tgtcaaagta aaagatgatg gggagtttga
    29041 gcgaggaacg cagattcacg gggagtggtg ctcctatctt tctgatcacc tccctgtcga
    29101 agtgatgctc cctgcctccc ctgttgactg ccatcggctt ctccatctcc aggagggaaa
    29161 cgagggggac cctggccgac acgaccccgt cgttgatctt tcccatctca aggctcatcc
    29221 accggcggag cacggactcg tcatacattg gaggattctc ctcctgacac acacactctt
    29281 ttcaccccgg aaagttgcat tgggcaagtg cgcctggacg gggcaggatg tcgagtgaaa
    29341 ctcacttcgt gattttcatc gtgtatgttc aacggcaggg cggtcatgcg cggttcctat
    29401 gaaaatgccc ccggcatttt catgcattgg acctgatgca cattgcatta ttcttgtttt
    29461 tatggatatc ctgccttttc atacgagaac ccgatccatc cccactattt ccgtcagaat
    29521 gtcccgggtc ttatgtccat gaggaaacaa cccgactcct ttccttgctg atctcacttc
    29581 ttcctccata agtcagattc ccgtatctcg gccttgcagt gggggcagag atacacctcc
    29641 ccatggtgtt cctgttgctc tttcttcttt gcattggtcc gtcgctcccg ttcctgcgtg
    29701 aatccggacg ccaggatccc ggccggaagc gcaaagatac cgatcccaag cagcgtgatg
    29761 cacccgccaa gtatcttccc tggagctgtc ataggaacca tgtcgccata gcccactgtg
    29821 gtgagcgtga caatgcccca ccacatggtg gcggggatgc tcgtgaagac ctcgggctgg
    29881 cccggctgtt ctatctggaa catgagcgtc gatgcggaga tgagcgcgat gaagaggaca
    29941 aggtatgcgg tgatcagcat atccctctgt gccttgacca cccggacgat catgttgatg
    30001 gagtcagaat agcgtacgag cttgaatatc cggaagaccc gcaataccac cagaccggtg
    30061 aggtttatcc cggtaaagat gttcaggtaa aaggggaata cgacgatcag atcgataagg
    30121 gcaaagggcg tgagagccca tgcgagcctc cccctgacag ggtgggaata actgggattt
    30181 gaagtgcatg accacagcct gagcgggtat tcgattgaga agacgagggt agtgaagagg
    30241 aagacaatct tgaagagcag tgaatacctg gccgagaggg agctcacgga tgagaggacc
    30301 atgacggaga tggagaggag gatgaccgcg gtgatgaaca cgtcaaacca gtaactgatc
    30361 cggtcacctt tgtacgtggg ttcaagtatc aggtatactc gctttcttat cgcggaaata
    30421 tccataatag ccatggattg agcgttcaat ttaaagattc tcttcgcctg tggaatggga
    30481 accagggtcc ggaatgaatg cggtcatccc ggtctgatgt ccaccagggc tgcaggaacc
    30541 ggttttgccc tcaaaaacac cttatagggg aagacccaac acaattccct gaggttgcaa
    30601 tggatatcat atcgcttgca gggttcctgg cccttttgat cataggaatt gcccttgtac
    30661 tctttctcat taagatcgtg tggtcgcttg tcccggcagc aattgtggcg ctggtggtgt
    30721 acctgtttac tgcaagcctc tggtggacgg gcgcagcatt cctcgccgta gtgatcctta
    30781 ccttccttta caagatcctt gccaaataag gagagcaccc aggcaatcgg gtttgggtgt
    30841 ttcttacccg ttgatcaggg tgctgatatg gatgaccttg atgaggtttc catcgcagtc
    30901 gagttcagga gtgcaggaga cgaggtagcg gcccttcatt gcctcgacct ccatctgcat
    30961 cgcttcatac tttttcgagg tgatcatcgc ttccagcggg caactgggtg gagggctctc
    31021 ccggtgatgg aagagttcgt aacacctctg tccaagcagt tccggtgcag tccgcccaag
    31081 tgcctcccgc gtggccctgt tcgccgcgag gacccggtga tcgggtgaaa ggattacaat
    31141 ggggttttcc acggcattga agatgtgctc ccactcccac tgcgcctggc ggaccatctc
    31201 ctcagccctt ctcttatcta ccgcctggcg tatcttgtgc gagagttctg cgaactgggc
    31261 aagggggtct ccacccttct ggatgtaaaa tgttgccccg ctcgtgaatg cctcgatcac
    31321 cacctcctcc cttcccctcc cggtgaacat gatgaacggc acatcatccc catcttttcg
    31381 gatctccttg aggagggaga tgccatccat gccgggcatc atgtaatcag agacaatcgc
    31441 atcgtatcgc tcctcgtgga tccgtttcaa tgccaatgtc gcggaatcaa gggtatcgac
    31501 tgaaaaatcc tcttcccgct gtaaaacctc tttccgagtt ccaggagatc cggctcatcg
    31561 tcgatataca aaagcgagat catgaattcc aacccacccc atgtgtattc ctccccggag
    31621 aagaaaaaca cgcatggttt aatcctgatc cctactctca tgggggaaaa aatgaataaa
    31681 cctgatgtct tcacatggag aaacggagcc acattcgctg gagcggcgat catgtcctgg
    31741 ataaatcgcg agaataccga caatccttac atgaagaagt gagaggaacg aatgggctcg
    31801 aggagattcg aactcctgac ctccgccatg tcaaggcgac gtcataacca gctagaccac
    31861 gagcccgcaa ttcttctgcg aaatgcgcgg agaaatgtgg gtgaacacag tataaaatta
    31921 ttctcttttc tcgcgtttgg aaagggaata ttgaagcgat ggcagggtga ctggaagagg
    31981 tgatggaagt gcgacacaat ggcagacatg aatgtttcac aactttttct ctacgagcat
    32041 gacgcaggga ggatagaact ccttgtccgg attgtatact ggattgccat cgggatcgtg
    32101 gcctggatat acgggctcat caccattatc tgcctcgttg tccagtggtt ccacatcctc
    32161 attcttggca ggaggtcgcg gggcctctcg gatttcgcga agggttacct cgagtacatg
    32221 gtccaccgga tgccatacat gtacattatg accgaccggc gcccggcaat catgcccgac
    32281 accgtgaaga tatacgagga gacgggctaa aagcccgcaa caaatgaggt cgcggatgac
    32341 ggtatgcccg ccccgcattc cttttctttg tccattcgaa ggaaaaaggg tgaggtatca
    32401 gatcagggta tccgagatct tggcaaacac tgcggtgaat atccccggca tcgacctgat
    32461 ttcatccatc atgtccgacg agacctcgct gtccacgttc aggaccatga tggcctcttc
    32521 tccgggtttg atccgtccga cctgcatgcc ggcaatattc acattcccct tgccaaggat
    32581 ggtcgaggcg cggccgatta cgcctggctt gtcgaggtgg cgggagacga tgacgtatcc
    32641 ttcggggatc atgtccatcg tgtatcctcc tatggctacg atccgtgacc gtcccttgta
    32701 aaagaccgtg ccgctcatgg tctcctccat ggtgtcggtc ttcacccgga tggtgacaag
    32761 gctcttccat ccacccgagt ccggggtcat tgtctcagat acgctgatcc cacgctcctt
    32821 ggcaatgaac tcggcattga caatattgat gggcacctgg agtatggggt cgaggaggcc
    32881 ctttaacgcc atgagagtga tgaagcgggt attgttccca agcgtcgcaa gctccccgct
    32941 gtaggtcagc tctaccttct ccatcctccc acccaccatc tgggtgagga acctgcccat
    33001 cttgtgagcc aggatggcaa acggttcgat gacgtcggcg tgctcggcag ggacgattgg
    33061 cgcatttacg atatagcgcg ccgaacctcc cttcaggacc gagaggcact gcctggcgat
    33121 agagactgcc acgttctgct gggcctcgac cgtgctcgcg ccgaggtgcg gggttgtgat
    33181 tacctggtcg agttcgagaa gaggcgagtt gagtggcggc tcctcttcga agacatcgag
    33241 ggccgcacct gcgaccttcc cagatttgat cccttcgtaa agagccttct cgtcaatgat
    33301 cccgcccctt gcacagttga tgatccggac cgcctctttc atggtggcga tggttttggt
    33361 attgacgatg tgtttggtct ccttgatcag gggggtatga accgtgatga aatccgagac
    33421 cttgaagagg tcttcgagcg agagtgcttc gactcccagc tgggcggcac ggtccttgct
    33481 gatgaaagga tcatacccca cgacatgcat tcccatcgcc tgggcacgtt tcgcaacttc
    33541 cctcccaatc ctcccgagac ctacgatccc gagcgtcttc tcgttcagtt ccactcccat
    33601 gaacctggag cgcttccatt ccttcttttt aagcgaggcg ttcgcctggg gtatgcttcg
    33661 tgcaagcgaa agcatcatcg ccatggtatg ctcagtggcg gcgagagtgt ttccttccgg
    33721 cgcattggca acgatgatcc cctttctcgt tgcggcatcc atatcgatat tgtcgacgcc
    33781 cgctccggcc ctcccgataa aacgcagatt cttcccggcc tcgatcaccc gcgaggtcac
    33841 ctcggtcccg ctccgcacca gcagggcgtc atactcgccg atgatggaga cgagcgtatc
    33901 ctccccaagg tcggtcttca catcgacagt gcactcctgt cgcaggatct ccagcccttc
    33961 ctcggccagc gggtcgctga ccagtactct gaacgtcact ataaaaccca cattatatat
    34021 gcatcaaact aattgagcgt ttttgtctcc ctgcgttatt tctcccatgg atgacatcca
    34081 ggtctcttca caggtgatga gaatgtatta aacagggagg ggtgcagatt atagtggtgc
    34141 gtgaatgaaa aagatgcccg atattctctg gcttgaggag atacggaaag aggatattgc
    34201 tgcagtcggg ggaaaaggtg cgtccctcgg ggagatgtct gccatcggtc tccctgtccc
    34261 gcgggcgttc gtggtcacca gcaacgcctt ccgccggttc ctgatcgaga gcggcattga
    34321 aaaggccctc tttgcaggcc ttgcaaatct cgacgtggaa gattccgggg aactcgagaa
    34381 ggcagcaaac aaggccaagg agttggtgat gaaagcccgc gtccctgcat caataaagca
    34441 gaaggtcaaa gaggcttatc taaagatgga gggcggcctg atggtggttg ccgtccggtc
    34501 aagtgcaacc gcggaagacc tgcccgacgc aagtttcgca gggcagcagg agaccttttt
    34561 gaatgtaaag ggcgagaaga acctccttct cgccatgcag aagtgctggg cctcgctcta
    34621 tggggcaagg gcaatctatt accgggtcaa gcagggcttc gaccaccaga gcgtgaatat
    34681 cgcggtggtg gtgcagcagc tggtgaacgc agagaaggca ggggtgatgt tcacctctca
    34741 cccggtcacg ggagagcctt tgagcatcat cgaagggtcc tgggggctcg gcgaggctgt
    34801 cgtctcgggc tcggtctccc ctgataaata cgtatacgac cagcgcagcg agaaggtcat
    34861 cgaccggctc atcgcgaaca agcggtacca gattctctct gacggggata acggaacaaa
    34921 gaccgtcgaa gtggagcggg agcgacagga cacccccctc ctctccgatg aagaggtgag
    34981 aaagctcgct acattcggca ggatcgctga gacccactac gatgtcccgc aggacgtgga
    35041 atgggcgatc gtgggagatg acatctacat cctgcagtcg cgtccaatca ctacgataaa
    35101 gcctccttcg gcatcaacgc ccgcccacga ggccggggcc gggaagatca ttctccaggg
    35161 ccagggggca tctccgggaa tcgcgagcgg caatgtcgtg atcataagtg acgcaaaaga
    35221 caccgggagg gtcagggagg gagacatcct cgtcacccgg atgaccaacc cggacatggt
    35281 ccctgccatg agaaaagtcg ccgccattat caccgatgaa ggcggcatga cctgccacgc
    35341 cgccatagtg agcagggagc ttgggacacc cgcgatcgtc ggtacgaaga ccgccaccca
    35401 ggtgctgaaa acagggcaga cggtcacggt tgatggtgaa aagggcttca tttatgaagg
    35461 gaggcttgaa gtcccgaggg agaaggccca ggctgcggtt acagccgccg tccccctcat
    35521 caccgctacc agcgtcaaag tcaacgtctc catgccggag gctgcggccc gggcggcggc
    35581 tacaggtgcc gacggggtag ggctgctccg gatcgagcac ctgatcctgg gcctgaacaa
    35641 gactcccgga tggttcatcg ccaacaacag ggaggaggaa ttcatccagg aactttacaa
    35701 cgggataaag acagtccttg acgcgttcta cggtaaacct gtctgggtca ggacgctgga
    35761 tgcccccacc gacgagttcc ggaacatgga aggcggcgag cacgagccga tcgagcacaa
    35821 cccgatgctt ggatggagag gtatcaggag ggacctccag tcccccgacc agttccgcct
    35881 ccaggttgaa acattcaaaa gactctggac gcagggatac gataacctgg gcatcatgtt
    35941 ccccatggtc tcccaccctt ccgagtttac cagggcacgg gagatgatgc agcaatgggg
    36001 tgtcgacgtt gaatcagcca ccctcggggt gatgatcgag atcccgagca gcgccatcct
    36061 gatcgaggac ttcatccgtg ccgggatcag gtttgcatcc tttggcacaa acgaccttgt
    36121 ccagtacacg ctcgccatcg accggaacaa cgagcacgtc gcagggatgt accagccgaa
    36181 gcacccggcg gtccttgcac tgatcgattc tgccatccgg ttctgcaggg agcatggagt
    36241 ggaagtatcc atctgcggcc aggcgggctc ggagccgccc atggtgaaat ggctcgtgga
    36301 gcacgggatc acgagtgtct ctgcgaatat cgacgccatc gcgaagatcc gcgagaccgt
    36361 tgccaggacc gagcagcaga tactcctgga gagcgcgaga tcccggaatg cgtgagattg
    36421 ggctctcaga ggaatcactc ttttcattcc tcctctcgaa gagagaggaa gatgtcgacc
    36481 atgcacatat cctgagttcc atgtgcacta tcccacaccc cgttgcggtc agggcacacg
    36541 tgcagttcat ggagacgaac ctcggggacc cggggctctt ccctggaacc gcatcgctcg
    36601 agcgcctcct tgtcgagcgg ctcgggaccc ttttccagtt gtccggcgcc tgcgggtatg
    36661 ccacatcagg ggggaccgag tcgaacatcc aggccctgcg gatggcaagg cagctcggcc
    36721 cgaaagagaa gccgaacgtc atcatccccg agtccgccca cttctcgttc aaaaaggcct
    36781 gtgacatcct ctgcctggag atgagggccg ttcccctgga tgaaaattat cgcatggacc
    36841 ctgtggctgc ggcagagatg atcgaccgta acacctgcgg catagtgggg atcgccggca
    36901 cgacagagta tgggatggta gaccccatcg ccgagctctc gaagattgca ggagaccacg
    36961 gggtcttctt tcacgtcgac gcggcattcg gagggttggt cctccccttc cttgatgatg
    37021 cgccggcttt tgacttctcc ctgcccggtg tcagcagcat cgcggtcgac ccgcacaaga
    37081 tggggatgag caccatcccg gctggcgtgc tcctcgtgcg ggactctgaa tctctctgct
    37141 cgctatcggt cgatacccca taccttaccg tgaaacagga gttcacgctt tcagggaccc
    37201 gtcccggcgc ctcggtcgca ggggcgctgg cagtcctcga atacctgggg attcaggggc
    37261 tgcgcgatat tgtggcaggc tgcatgaaga atacacgcag gcttatcgac ggcatggagg
    37321 cctacggcat catgcgggcc gcgactcccg atgtgaacgt ggccactttc tcccctgccc
    37381 gcatcccacc cccctggaag gtgtcctgga cacggagagg ggatctccgc ctggtatgca
    37441 tgccgcatgt gcatgccggg gcggtggaat cgtttctgaa ggacattggt gaacagaatg
    37501 cttgaacgac ttgttgcatc actcgagacc tgcccccttg tgaagcgggg ggagtataac
    37561 tatttcatcc accccatcac cgacggagtt ccggaggtgg acccgcatct cctgcgcgag
    37621 gtctgcatgg cgatgatcaa ggctcttgac cttgaggggg taaaccggat tattgccgcc
    37681 gaggcaatgg gaattcccat aggagctgtc ctctccctcg cgactgatct cccgatgaac
    37741 atcgtccgca agagggagta ccagatcccg ggcgaagtcg ccgtccacca ggcaacaggg
    37801 tattcgaagg ggcagctctt cctgaacgga gtgaaacgtg gcgacagggt ggtgatcgtt
    37861 gacgacgtga tcagtacagg gggaacaacg agagcgctgc tctgggccct cgatcaggtg
    37921 ggagccgagg taagggacat ctgcttcgtc ataaacaggg ggtcgcccga catcgggagg
    37981 ccctataaga ccctggtgag cgtcgaggtg acagacaggg tgcgtgtgat tgacaggcta
    38041 ttctgacctc gcagaacggc tcagggccag gggggccagg gctgtcgctc tccagttccc
    38101 tgccgggttg aagagaaaag caggcgtcat cgcgcagttc ctgaaggagc agggctttga
    38161 ggtgatcgtg agcggcgacc cctgctacgg ggcatgcgac cttgcccgag agacgctggg
    38221 atatgcagat gtcctggtac attttgggca tactcccctc ggagaccctg acccgcgggt
    38281 catctttgag ccttacaggg tagatttcga cccagaaatc ctgaaaaatg caatcccatg
    38341 cctgaaagcc acaagggtcg gtctcgtcac caccgcgcag cacctccacc tccttgatgc
    38401 aatgaaaacg gtgctcgcgg caataggtgt ggaggcagtg accacgaggg ggacaagggg
    38461 ctgcggggag ggccaggtac tcgggtgctc atttggggcg gcccgggtca ttggcacgga
    38521 agagatcctt tatgtcggca ccggtgtctt ccaccccctg ggggtgcagc tggcaacagg
    38581 cctccgggtg gttgcactcg accccttcac gggcgaggtg caggaggtga atgcagagaa
    38641 gttcctccgc cgccggcatg cattgataga gaaggccagg tccgcggatc agttcggcct
    38701 cctggtcagc acgaagaccg gccaggaacg atacgaaatg gcgcgcggaa tggctgcccg
    38761 gtgcagcagg gcaatactca ttctcatgcg ggagatctct cccgaagaac tcgtcaacct
    38821 cgggtttccc gcctacgtga acttcgcgtg cccgaggctc gcctatgatg accaggtaag
    38881 gttcccggca ccggttctca cgccccagga gttttcgatc cttcttggcg agtgcctgtt
    38941 ctcggactac cggatcgacg agctcacatg acaggcatgc gtctgaaacg gctcgagatg
    39001 ctcctctcaa gggtgaaggg gtttcctttg ccttcagcgc gatacgagca gtacctcact
    39061 cctcccgacc tcgccgcccg tctcctcttc cacgcccaca tgcgcgggga tatcgcggag
    39121 aagagagtct gcgatctcgg gtgcggcacc ggcatccttg ccatcggggc cgcgctcctc
    39181 ggtgcggcgg aggtggtcgg tgtggaattc gaccctcgcg cactccaaat cgcctccgga
    39241 aatggaaaag aactcgatgt cttcccgcgt ttcatcgagt tgcacctccc gaacccagac
    39301 ctcgcttcgc tggtcggccc gtgcgataca gtggtcatga accctccctt cggagcccag
    39361 gaaccccatg ccgaccgacc ttttatcgac gcggccctct ccattgcccc tgtgatatac
    39421 gggatattca atgcggggag cagggacttt gtgaaagcgt atacagaaga gagggcgaag
    39481 gtcgaggagg tggtgggggg caccctctcg atgaagagaa ctttctcctt ccatgcaaag
    39541 gatatccggg agactcctgt ggagatcatc gtgttgagga ggcgcatgaa ctgaaatcac
    39601 tcaccgggcc catattcagg ctattcctgg ctcacatggt caccgacctc tatatgccgg
    39661 tcattgcggc cattctcccg ctgctcatct ccactcgggg gtattcctat ctcctggcag
    39721 gcctcctgat cacctcctac aacctcacct cctcggcgtc ccagccagtc tttgggtggc
    39781 tttcggaccg gaaaggctgg caggtccata tctccgtctc ggtcctcatc tcggccgtct
    39841 ttatcgggct gatgggggtt gtcgagccct accctattct catagcctgt gccgcgctgg
    39901 cagcgcttgg tcacgcgtgc ttccatcccc atgcccttac catggtgagc cggcttgcga
    39961 ccagcctgaa cagagggaag gttactgccc tctttgtagt agggggaaac atcgggtacg
    40021 cgcttggccc catcctcgca ggggcggtca tcgtcacctt cggcctccct ggcctccccc
    40081 tcctcgtgat ccctgccctg gtgatggctg tggtgctcca taagtcgtca ccccaccccg
    40141 gcgaacaccc cgcatccttg gagagtcccg caaaggaccc ggtatcgctc gcctccttct
    40201 ccccggtgtt catccttttt tcggcctcca cgctccgtgc ctgggcggtg tttgccgcga
    40261 tcgcattcct tccccccttt cttgtccttc gggggctcga catcttcact gcaaacctcc
    40321 ttgtctccct gatgctcctt gcaggtgtcg ccggtcaggt gtcaggcgga atcctggcag
    40381 acatctacgg aaagaaggag ttcgtgatcc tcggccttgt cttctcgatt cccgcgttct
    40441 ttgcctttct tgcaacagac ggactcccct cgatcgccgc gctcctcgtc ttcggatttg
    40501 cgctctggtc gagttttgcg ctcaccgtgg caatggccca tgaaatgatg ccggatcacg
    40561 tgggcctgac ttcggggctg atgctcgggg tggcggtggg agcaggcggc ctgggagttg
    40621 ccgcaaccgg catgatcgca gatcgtatct cccttgatgc ggcgctgtcc accatcccga
    40681 tcctcgtcat cgggtctctc ctcctgatgc tgctcgtgcg gtatccatgg aaatcgctct
    40741 cccttccggg ggcctgaccg ggagacgtgt cttcgctctt actttttgaa gagggaatgc
    40801 gcaaccgcat gcctgatatg ccgctctgtg agcatccaga gtgccggcac aagcaccagt
    40861 acgagaaggg caaacccgac cgccgagaag aggaggaggt tgaagaggga atgggcggcc
    40921 atggcgagca cgaggccgtt tcggaccagt gcacttccct cttgttccgg catgaacctg
    40981 gccatgccga gcgcagaccc ccacatcact gaaaagagga catgcccggg gaccgagatg
    41041 agcccccgga caacccctgt ctggagcgca aggaggaggg actcatcgaa tgccgagaag
    41101 aggtagagga tgttttcaat ggtcgcaaac ccgagacctg ccgcggcggc atagatgatc
    41161 ccgtcgaccg gctcgtcgaa cacccggctc cggtagac
//
LOCUS       DAOQYR010000011        41014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_1080_length_41014_cov_8.190532, whole genome shotgun sequence.
ACCESSION   DAOQYR010000011 DAOQYR010000000
VERSION     DAOQYR010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 41014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41014
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1080_length_41014_cov_8.190532"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            851..1126
                     /locus_tag="PKM87_02495"
     CDS             851..1126
                     /locus_tag="PKM87_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28965.1"
                     /translation="MITASPFGEEETGPEDAERGGYSPIRRRDEKGRGVASSQMPDAK
                     GRYQRFSWFFKVRKIKTISEKPYLISHYFTFDSSVRYKRTKKDAHII"
     gene            1206..3392
                     /locus_tag="PKM87_02500"
     CDS             1206..3392
                     /locus_tag="PKM87_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ28966.1"
                     /translation="MSVLFVDNEPHILDVTKITLEKSGLFSVDISESASAAMEKLKHA
                     SYDAIVSDYQMPDMDGIEFLKKLRDSGNNIPFIIFTGKGREEVVIRAFENGANTYVQR
                     GGDSNAQYAELKQKILSAVKAYRDEKALKESRERLRALIEKSSDLIRILDRDGKIIFD
                     TAASEQVLGYPPGYTLGRSPLEFIHPEDVEYVRRELFTVYEHTNTGVPARFRIRRSDG
                     TYTWVESVGQNYIGVPGLDGVVINTRFIDDRVAAEQALRASEERYRTFVENASEGVVV
                     VQDGLVEYANPAALGLVHVTLPNIHGHPISEFIHHDDQSMVNERYRKRILGEPVPHTY
                     DLRLIGKDTSEIWVQLSAVRITWKGRPATLNFLSDITRRKAVEQELTKERQRLFDVLE
                     TLPVMICLLTPDYHVPFANRAFREKFGEPQGRHCYEFCYVQNEPCSFCQTYNVFKTGK
                     PHNWEVRLEDGTYIHAYDFPFTDTDGSPLILEMDIDMTEQKLIEAEIRSLNQVLEQRV
                     DERTAQLTALLKERELLLREVHHRVKNNLQIIISLLNLQSRYLTDEKTLTAIHESQNR
                     IRAMALVHERMYRSEEISHIAFKDYVKYLISHLFGFYGVETRRVSYTVSMEGLPIGID
                     TAIPLGLIMTELVSNSLKYAFPGSKKGTISLEGSALDDHTYRFIVKDNGVGIPEEIDW
                     RNPDSLGLRLVNSLVTQLNGTIELDRTDGTTFTMMVHSKERNGKGG"
     gene            3591..4193
                     /locus_tag="PKM87_02505"
     CDS             3591..4193
                     /locus_tag="PKM87_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28967.1"
                     /translation="MNKYTRNIGYIAISAVFFSFLVFLITNSYPYEIFYFGKERYDAI
                     SFFLGLVSCGGVFVFLYSIKNDVQMWHLGLPLLVTIVVVTISSLLSPIFPEYFGYPMD
                     GNATPFNLLSGQIMPYLLAPFSALFFYSLKEMKGDVRILTLISFGISILFSGSLFIEI
                     PMYDVSMSFLLYWVYGMPLIGALYLASCTGFDYHKPQRPN"
     gene            4889..6262
                     /locus_tag="PKM87_02510"
     CDS             4889..6262
                     /locus_tag="PKM87_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HNQ28968.1"
                     /translation="MTGQGPDACLEGILALSGDDRWKIDHSHAVINVTAYVVRSFDGN
                     ALIFAMPLHVLRPLLSEVPLDLRGSPGAVACIIEQIKQRAQSDPALGPLVGRGTRFPH
                     QFASITEPYTVVGSSAALASDLWHAGDRNFADASGVHLLLNGAVPCPQQFTQADVASV
                     IETVARLCDAVTAIACFVPVRELETAWISTLDQQLLREMLPSLGLVSFIGDGARLARH
                     YTRYRCYFRTAGPKTGVHIPFACPLELDPCELELPASNRTITGLGIRRREVFAVAGSN
                     AQGKTTFLEGIHAGMDDHATGDGRELAVTVPGLCTAEAMNCMLTGADVSMFFSALPPG
                     ISGTAHAASGMGSGSMNMAYQVQRAIGRDCPLLVIDEDRAAPNLLVRSCLQTHEITPL
                     SEILGHDRGKMGETALIFAACAMDVLVAQADRIMLLDNHTAYAVDREVFRKRVAESLE
                     KIAGDLR"
     gene            6638..8152
                     /locus_tag="PKM87_02515"
     CDS             6638..8152
                     /locus_tag="PKM87_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HNQ28969.1"
                     /translation="MEPSVEYILLVIAVLILVSILANKVSGRLGVPALLIFLVVGMLA
                     GSEGPGGIYFDNPWIAQAIGVIALAYILFSGGLDTRWSEARPVLPQAVVLSTAGVLLT
                     ALLMAAIAVVVFKVTLLEGFLLGAVVSSTDAAAVFSILRTKRAGLKGILRPLLELESA
                     SNDPMAVFLTIGAITLILNPGMPVLAMVPLFIQQMAVGGLIGYGMGRVVVYLINRLKL
                     EFEGLYPVLTLSLVMMTFGLTASLGGSGFLAVYIAGLVLGNSSFIHKNSLMRFHEGIA
                     WLMQIAMFLTLGLLVFPSHLIAVAGAGILFSFFLIFIARPLSVFLVLSPWKIALRDKV
                     MVSWVGLRGAAPIVLATFPLLAGIPQAGTIFNLVFFIVITSALLHGTSIPFIARRLGV
                     SSPLQDSPKLAMSLVAGCDSGNDLVEIVVSPGAVVAGKQIVEIGLPPETLIIMMEKSG
                     RRFVPCGSTIIEAGDTLLILTSPELGGRVRSLFSAAHPGTGVTARETDEEKDTI"
     gene            complement(8450..8815)
                     /locus_tag="PKM87_02520"
     CDS             complement(8450..8815)
                     /locus_tag="PKM87_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ28970.1"
                     /translation="MSKSRIFIVEDDDVIARLVEWRVKKLGYDVCGRAAEGKEAISLI
                     QSMSPDLVLLDIGLPGNCDGISVAHEIRRTHDLPIVFVTGYSQGEIIDRAKKANPNGF
                     ILKPFSDDDLRVGIELALR"
     gene            9122..9817
                     /locus_tag="PKM87_02525"
     CDS             9122..9817
                     /locus_tag="PKM87_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNQ28971.1"
                     /translation="MKYEIKYKPSYSLLVVTLAPGESIAAESGSMTYMQPTIEVKTQK
                     REKSFWGSLGLALVGGQSFFINEFTATTGTGEAGFSAAPLGDIDTIAVSQGKGFILQK
                     SAYIASQGTVNLDVKWEGFTKGLFGQGLFMIRATGEGTIFINTFGAIDRHTLKDGEKL
                     VVDNFHLVAFSDTCQYKVTKFGGLKETILGGEGLVTEIQGPGEVFIQTKNIRELADWI
                     WTIIEPRVQAKAR"
     gene            9962..10480
                     /locus_tag="PKM87_02530"
     CDS             9962..10480
                     /locus_tag="PKM87_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HNQ28972.1"
                     /translation="MESQHEGRVPDGTGWIRSPLKLLAAVIICNMAGIIGSFVTVTGE
                     GSWYAAIQKPPFNPPGFVFAPVWTTLFVLMGISLYLIWMEGMERREVRVAIALFAVQL
                     ILNTIWSFLFFGLQSPFLGLVEIIILWIFIVATTVAFYRINKKAAYLMVPYLAWVTFA
                     AFLTYNIWLLNG"
     gene            complement(10638..11108)
                     /locus_tag="PKM87_02535"
     CDS             complement(10638..11108)
                     /locus_tag="PKM87_02535"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HNQ28973.1"
                     /translation="MQIHRIILLSALAVLACASAPVMAATYQFSLENNGDSVNVIPGD
                     TITLSLKENPSTGFRWIMETSGGLQTLRDTYSPSSTGLIGAAGMRTWKFLVTGTGTHT
                     ISAIYKQSWMPTTGDEQKFTLDVISGYSAIQKPVSTSKFRSYQKPILYKTVHYP"
     gene            complement(11537..12277)
                     /locus_tag="PKM87_02540"
     CDS             complement(11537..12277)
                     /locus_tag="PKM87_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28974.1"
                     /translation="MKKDICDFTATLCHFMVPLPVIEVGEKEFQLSSFLRAYWFLFIF
                     VSIFTALTAFLTQTFSGHGSREPIFELLGIPFDLQEISIASCLLITYLGVFALLWAAF
                     AEPRDRPIFYYFIGVEAFKRLLLVIPLVVLMLTLTTWINLVHTQVMAVIFSLIGFCLG
                     IVVFSSILVLVSRNTRIRRFVLATMTYALVVFVVSLASLLAINLRSVPLVYTPAIFFI
                     YAMGISSVFYIIATLIFEIILPLFQGWK"
     gene            12391..13167
                     /locus_tag="PKM87_02545"
     CDS             12391..13167
                     /locus_tag="PKM87_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ28975.1"
                     /translation="MISILLVDDEVQFLEVTRMFLEKGRGIRVTTATSGDQALEVLAH
                     HKFDAIISDYAMPGMNGIEFMKQVKALGDDTPFIIFTGKGREDVVIDALNFGADLYLE
                     KSPDPRTQFSEMMQKVRNIVTIRADVQVAREKMRIFSSVMERFPGMIFRMKLNGRWAF
                     LFASAGADRLTGYSGDTLVKGDPAFEMIVIPDDRERVDQSRRSALDSGGEYQVKYRIR
                     TSLGEEKRVVEGGFVVPGSDEEILEGYILDLDIVGNGSVR"
     gene            complement(13209..15014)
                     /locus_tag="PKM87_02550"
     CDS             complement(13209..15014)
                     /locus_tag="PKM87_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ28976.1"
                     /translation="MMQILLVDDDPDLLDLICRFLEKEEEMRIDTCMSGTEALDALNT
                     THYDAIVLDYELPGMNGIDFLRMIKGRGNDTPIIVFTGKGQEETVIKALNYGADYYLQ
                     KRGDPRLNFLELLYMIREAVRRHEARKELLESERRFRESLENARLIAFQLDRNLSISF
                     CNAFLLTLTGYEKADLVGKSWTETFIPDNEQRMVEQFFLSAMDGDLNALYFTCSIRTR
                     EGKVRKIDLNNTLLCDLEGNVTGISCIGQDITDKVIMEGQKAASEERLRLLFEYAPYA
                     YFLTDLRGTFVDGNFAAENLIGYPRRDLIGKNFLEAGILSPEEVPKAIGLLARHVEGT
                     PTGPEEFVIHKKDGSRVVAEIRNHPVVIGGKTVVLGSAHDVTSRHMAEESLRRMNAKL
                     TLLNIITRHDILNTVTALLLYIELLKEDPKDDAQEETLSKVEQLTQTIRRQIEFTKVY
                     QDIGIEVPQWQSLGEIIGRASASATMKDVAVKSDIGDIEVYADPLLENVIQNLIDNSL
                     RHGQGADSIWLTVLHDGQGMKIVYEDNGQGIPSREKEEIFKRGYGKNTGFGLFLAREI
                     LAITGLTIKETGTFGKGARFEIRIPQGCFRQAQKQ"
     gene            complement(15324..15659)
                     /locus_tag="PKM87_02555"
     CDS             complement(15324..15659)
                     /locus_tag="PKM87_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28977.1"
                     /translation="MRQAPVSVFTRAGTARRSGKGMSLSLGDRIFYVPPEEVGGLFSD
                     RNAMILNDEGEQEGIAWLSPVLARKKQDLTALIHDRLFVVSCRDLHALLSGDREGIIV
                     REYNAGRAA"
     gene            complement(16183..17919)
                     /locus_tag="PKM87_02560"
     CDS             complement(16183..17919)
                     /locus_tag="PKM87_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2070 family protein"
                     /protein_id="HNQ28978.1"
                     /translation="MSVMGGELKIERLGRYLFNAPSWPISLALLLLLGFILDGVSLKL
                     GSPLWLGTRAFTIPALFAFLVTKPAVELIGRPMTWNRSALLALSCTLFGVIITLSSLT
                     ISQSMLPLFYAISLGIILGLRLLVLVAIADYRVLRMILPAASQSLAGIAMGMVLFPGN
                     FFILALVLQVFFGLGFILLVWAIDRPLYRTFQIRGLDFLNSFLAHLTDGSKALEDFFR
                     KIGEEVTVPQVTLFFRRNEKTGLLLTVPNVHPGPMGEIGGGNLPRGMQSGFSEMVMVP
                     HGAATHDFNLVSEQEIDKLVQAVAASRDNLVFSSSATRSVRYQEGSVSLLCQVFRDTL
                     LMVSTRSPERTEDLDFNIGMTIMAEGHRSFPHIAFIDAHNCYAGDITYVLPATRLAME
                     YYHAGIKAIDETPGMPRFSLELGIAHVQVPFSREQGFGDQGIQLAVVRAGEQSTAYLL
                     IDGNNMESGVRETIRDFLLESVDEAEVMTTDSHVVNTVSGKNPVGLHVPVTEIIPCVD
                     AALRQALADMAPAETAGSTAWCEGVVVFGSHRITQMASTVNTILLFIPVLSAGMLLLV
                     FLLSILVYVMIG"
     gene            complement(17926..18846)
                     /locus_tag="PKM87_02565"
     CDS             complement(17926..18846)
                     /locus_tag="PKM87_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNQ28979.1"
                     /translation="MIYVVGHTAIDHICRVPAFPPRNGSATITERKVYFGGGAANIAA
                     GIARLGEPCTLVSAIGGDFPGSEYDRWQKSLGLNQRFFEVPDAHTATAFMFTDEDGDQ
                     ITFFEWGASRIFSDSEAPALPFVHMATADPDFNTRVAEKAAFSTFDPGQDLHRYSRTQ
                     LDAILSHINILFANQHEVESMCRTLGLSREGLAARVPLAVFTMGAGGSEYFHGEECSH
                     IPAIRVRMADPTGAGDAYRAGFLAAFRRGYSPRDCCRIGTVTASFAVEVTGCQNNLPS
                     WEEMETRFRQVFGDVPVIEPGAGEAQRSRV"
     gene            complement(18843..19181)
                     /locus_tag="PKM87_02570"
     CDS             complement(18843..19181)
                     /locus_tag="PKM87_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF555 domain-containing protein"
                     /protein_id="HNQ28980.1"
                     /translation="MPDYRVILEGAWVVKDVSSLEDAVSIAISEAGKRLNPVAAYVEV
                     EVGMLACPFCEGELNSALVVARTALVGLLLEMKVFRAESPEHATRIARSVIGKALRDV
                     PLEVKEVCAL"
     gene            complement(19192..19719)
                     /locus_tag="PKM87_02575"
     CDS             complement(19192..19719)
                     /locus_tag="PKM87_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNQ28981.1"
                     /translation="MDSSEFLQFLVSRCSVREYDTREVPDADLTYILDCASTAPSAGN
                     LEAWDVVIVDGQEEKERVAEASFGQEHVVSAALLLVVCANYVRSMSRYGDRGVLFAIQ
                     DATIACTYMMLAAHARRLHTCWVGSFQDDEVRDMLDLPPHVRPIAILCVGKGFSPGSF
                     TERMPVVEHVHHQLW"
     gene            complement(19706..20062)
                     /locus_tag="PKM87_02580"
     CDS             complement(19706..20062)
                     /locus_tag="PKM87_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ28982.1"
                     /translation="MDLKKAVKGYQFAERAKSELIISSQLTIALAGFPESERAGGKRM
                     LVLLLEAVRSELEFAWKGTEMSDFRRAINLLSESISTVESENYGAASLKISESISAVT
                     TAAQGSWQVLEAHGLI"
     gene            complement(20151..20480)
                     /locus_tag="PKM87_02585"
     CDS             complement(20151..20480)
                     /locus_tag="PKM87_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5350 domain-containing protein"
                     /protein_id="HNQ28983.1"
                     /translation="MGKTGSVQWTQVKGVKGQIRLVPKSEGELKKPGPNQRFKAGASL
                     RKLEQLTGEGQGGFRGGRGGRGGRGGRGGRGGRSSESGPQANPMVRKAMRRPKVSVLG
                     AKQKSGR"
     gene            complement(20552..22420)
                     /gene="gatE"
                     /locus_tag="PKM87_02590"
     CDS             complement(20552..22420)
                     /gene="gatE"
                     /locus_tag="PKM87_02590"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HNQ28984.1"
                     /translation="MDYNALGLVAGIEIHQQLNTREKLFCRCPTLLRPFEEHDGEFSR
                     YLRATESELGEIDRAAREEMKNFRRFLYYTYDSTCLVENDEEPPAPLNPEALATCLQI
                     AKMFGMAPIPQVHTMRKLVIDGSNTSGFQRTALVAVNGTLPNGGTIETICIEEEAAQR
                     VKDEVFSLDRLGIPLVEITTSPCMHTPEEVQEIAEYLGMVLRSTGKVKRGLGTIRQDI
                     NISISGGARVEIKGVQELDLIAEVVRREVERQERLLSIRDRLKERGASVSGTPVDVTE
                     IFSHTGSGILKKASRIMAVTLAGFAGLVGDEIQPARRLGSELSDYAKKCGVGGIFHTD
                     ELPAYGVNAEEVTALRDMVGAGESDCIVIVAGTPRQAGCACQQVIRRAELALLGVPEE
                     TRKMLEGGSTSYMRPLPGAARMYPETDVLPVTISPEYWEDIPRVELLQAKAERYMTAH
                     GLDAAVAKQVAYSEHLPLYERSVGLGIPATLSARTLIATLKELSRDGLDIRPLVADCD
                     AAGNAVMPAVLQVLKGVVEERVGKEAIPDVLRRIVQGESAETAMQAVEATVAGTDLDA
                     LARRIVSERMTFVSEKGMGALGPLMGVMMKEVGGAVDGKKVSQVLKAALDQALSGR"
     gene            complement(22410..23645)
                     /gene="gatD"
                     /locus_tag="PKM87_02595"
     CDS             complement(22410..23645)
                     /gene="gatD"
                     /locus_tag="PKM87_02595"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HNQ28985.1"
                     /translation="MSGFLPGDLVNCTVGGTGRRGTYITDRDGMAVLKMDSGYNIGVF
                     PGDCTLIERPSPLRVERAEVEQDPSLPLISIVSTGGTIASRIDYRTGAVTSQFDAADI
                     LRAIPGLSAIARCRTRKLYTILSENMTPSIWQELARAVYEEIQDGARGVIVTHGTDTM
                     AYSAAALSFMLDTPVPVVFVGSQRSADRPSSDNVMNALCSASTAVGDLGEVAVVMHET
                     TSDDRCAIHRGTRVRKMHTSRRDAFQSIGAPPLGFVDYPSLEVKLSPGAVRRGACEPA
                     LHDRLEPSCGLISFFPGMSPDLISSCADVKGLVIAGSGLGHVSTSLIPAIRELIDQGT
                     LVVMTSQCLHGRVCDRVYDTGRDLIRIGVVEGEDMLPETALVKMMWVLGNEPDAERAR
                     CMMQENQKGEYTRRSLHGL"
     gene            complement(23642..25123)
                     /gene="argH"
                     /locus_tag="PKM87_02600"
     CDS             complement(23642..25123)
                     /gene="argH"
                     /locus_tag="PKM87_02600"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNQ28986.1"
                     /translation="MEQDVVRRGRLTGERSGEMMHFLSSMGADRCIADADILVDIAHV
                     LMLSRQKIIGKEQAGRLLETLLELYENGIPEAAFDDRFEDIHAGIETLLIATIGEDAG
                     GRLHIGRSRNDEVATCIRLRVRDDLVRQLSILIRMREVLLDLAGEHIESVMPGFTHLQ
                     HAQPTTLAHHLLAHEAAFARDYQRLSGALSRVDWCPLGSAAFASTSYPVQREFVAGLL
                     GFQGVLENTMDAVSSRDFALEVLSCLSIMMTHASRVCEELVVWSSGFARFVELDDAFC
                     STSSIMPQKKNPDTAEVMRAKAASVLGSATASFAIVKGLPLSYNRDLQELNPHLWRGI
                     RDAKHSTRLLIDMLSSATFNTAVMAGEAGKGFSTATDVADMLVQSHGFAFRTAHTLVG
                     KAIQKGALDIPALDQAAEEMGIQPPSKMGIREEEIAAALDVRACIERRRAPGSPSPFA
                     VRISLSSRRTALHEDLRSLEERMAHYAESVRRLISDAKKEVGK"
     gene            complement(25167..25697)
                     /locus_tag="PKM87_02605"
     CDS             complement(25167..25697)
                     /locus_tag="PKM87_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ28987.1"
                     /translation="MQRDPVDRLVNAALISDDEFVHALREVMQRDLRISVRELAEKSG
                     VAPSSLYKMLHGKRSPNLTTLRAIVQAMRRITLSSEGEFIGLIAARPVLDVILERNAE
                     VDGRKVRIREYPVHTMEEAIVAAVRAEREGAVAIVCAPIVSSVIEQLVKVPVATIIPR
                     ESVQRAIELAARKAWL"
     gene            25887..27122
                     /locus_tag="PKM87_02610"
     CDS             25887..27122
                     /locus_tag="PKM87_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ28988.1"
                     /translation="MKYSHVCAIIGVILCAALIAGCITPTQHQGTPTPTVSPSGGDVI
                     VTYTKGTGPMPTLLGTDQIDGYIAWQPFVEVPPMAGIGKILLYSGDLPPQGKWKDHPC
                     CVFTANQKVIAQNPDLTNALTASMILATQYLEEHPEESAEIVADWLAGKGNFTYGEIS
                     VSSVDVLKRAFPTVKFVNDPTDKWMDSNIEFVHALRELEVLTGSLKASSDADSKTILF
                     DTAPYNAAQSMIGAGKITTPTPVKGSVGIGYLMSDHHAALFVAIKKWEYFNDTYGIAI
                     KPRDTAASRPDIADFIVNGEKVAEIKLISGDAGPQLMQLMATDNIQYALVGNPPAISA
                     ADKGTPVKIIMSINSEGSGVVVTEDSPANDWNSFVAWAKERSAAGKPLRIAAPGKGSI
                     QDVMIRYSLETSGLSIKEG"
     gene            27135..27899
                     /locus_tag="PKM87_02615"
     CDS             27135..27899
                     /locus_tag="PKM87_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ28989.1"
                     /translation="MRYLRFLLPILLVIGWEVASWVVNNPFIVPSLETVIPILLHPFS
                     PEYTLGTGSMVENALISIQRVGLGFLVAAVVAIPIGILMGRSAFLNDFFDSTIELLRP
                     IPPLAWVPLALAWFKIGLTSIVFIIFIGAFFPILLNTVDGVKSVKKTWLEVATTLGAK
                     EQQIMTKVVLPGAAPTIWTGLRVGFGIAWMCVVAAEWLPGISQGLGYLILYAYNFGQT
                     NLIIAGMVVIGVIGLLIDFVFKHVEKRWFSWRGLER"
     gene            27931..28701
                     /locus_tag="PKM87_02620"
     CDS             27931..28701
                     /locus_tag="PKM87_02620"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ28990.1"
                     /translation="MPVTLVLKDLSKSFAGEGKAEVEALSHINLEVSDGEFVSILGPS
                     GCGKTTLLRIIAGLDHATGGTILLDGNKIEGPCPRMAMIFQEYSLYPWRTVIDNIAFG
                     LEMKGMEKQKRREMAFQFLELVGLVDFAGSYPYELSGGMRQRVAVARALAIEPQILLM
                     DEPFGALDAQTRNMLQKELLEIWQKTRKTVIFVTHSVDEAVYLSDRVVVLTARPGTIK
                     DMVHIDLPRPRERTGAEFARIRRYVLDHVEKTPFSSKV"
     gene            28755..29291
                     /locus_tag="PKM87_02625"
     CDS             28755..29291
                     /locus_tag="PKM87_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNQ28991.1"
                     /translation="MVRKPGKMYRNISKKAYTRREYMGGVPGSKVVQFDMGNLSGEFP
                     MEVSLAVEEACQIRHTALEAARITINRHLIKDLGRMNYRLKLRVYPHHVLRENKQATG
                     AGADRVSEGMRLSFGKAVGTAARVAPGQEIFTVYTIPQYLPQVKTALKSGGHKLPSPS
                     YINISERTVPESPAPQQA"
     gene            29485..30321
                     /locus_tag="PKM87_02630"
     CDS             29485..30321
                     /locus_tag="PKM87_02630"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNQ28992.1"
                     /translation="MIPSPGPALFAAVTKATCEAFRRAEITLPSDVIEALRNARESET
                     SEIARGELANILANLEMAERLSVPICQDTGVPVVYLTIPPHVPLTTEIYGAIAEGVRM
                     ATEEVPLRPNVVDPLTRENSGDNTGTGMPAIHVRPGDSLTITVLPKGAGAENMSRISM
                     MLPSEREQIPKFLAETMLIAGGKPCPPVILGVGIGGTFDEAASLAKEALLDPIDTMTP
                     YEQELLDAVNMLGIGPMGLGGSTTALAVKVKTAGCHTASLPVAVNVQCWACRRATVEV
                     PT"
     gene            30327..30884
                     /locus_tag="PKM87_02635"
     CDS             30327..30884
                     /locus_tag="PKM87_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HNQ28993.1"
                     /translation="MDLCTPLSHEVLMLRAGDRVTLSGTVYTARDEAHLRMMEEGIPF
                     DPHGAVVYHCGPVIQGQRVIAAGPTTSARMNSLSGFLIEKGVRALVGKGGMGRQVVDQ
                     LKGRGVYLAFTGGCAALAASRMTLKGVFFEDLGMAEAVWAIELDRLPLVVGIDAAGND
                     LFEGVHRNAQAGFKKRFQARRDVPA"
     gene            30881..31153
                     /locus_tag="PKM87_02640"
     CDS             30881..31153
                     /locus_tag="PKM87_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNQ28994.1"
                     /translation="MRLVIDERRCKGCNLCTLVCPYRIFTPGTQPNARGIVVPVLDRP
                     ERCANCRLQALYGRTLCGMCQMICPDQAISWVEEKPGEARKVAIEF"
     gene            31150..32250
                     /locus_tag="PKM87_02645"
     CDS             31150..32250
                     /locus_tag="PKM87_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNQ28995.1"
                     /translation="MTRIDFMQGNTACAEGALAAGCRFFGGYPITPSTEVAEHMAAKL
                     PKVGGVFIQMEDEIAGIASVIGASWTGARAMTATSGPGFSLMMENIGFAVMTETPCVL
                     VNIQRGGPSTGQPTMSAQGDMFQCRFGSHGDYSIIALSPGSVQEMYELTVKAFNLADR
                     FRVPTFLMADEIIGHMRERIEIPDSVPRVERKPLVKGVLPFHADDDMVPGFPQFGTGH
                     HVHVTGLTHDERGYPCATSPSLHEDLVKRLVAKVESARHEIADYKVTNPDAEVVFITY
                     GSPSRTVDQVLHDRQAERIGHLRFRMVWPFPENALEQFPNAKVFLVPEQNLGLMAREI
                     ERHTCVPVVPLPRFGGALHTPAELEKAMEAWR"
     gene            32247..33032
                     /locus_tag="PKM87_02650"
     CDS             32247..33032
                     /locus_tag="PKM87_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNQ28996.1"
                     /translation="MTYDDWLRADRLPHIYCAGCGNGTVINCTLNAVDRMGWKKEETI
                     FVSGIGCSSRAPGYIMTDSLHTTHGRALAFATGIKMANPDLHVVVFTGDGDLAAIGGN
                     HFIHACRRNIDLTVVCMNNQIYGMTGGQGSPTTPRGALSTTTPYGSQEPAFDLCELAS
                     AAGANFVSRWTSYHVKELTRSIHEGLGIPGFSFIETLVQCPTNYGRRNKFRQVMDQVE
                     YFKAHGILVQKAARLREEGKAIPGDAIILGDIVRRDRPAMGVR"
     gene            33032..33571
                     /locus_tag="PKM87_02655"
     CDS             33032..33571
                     /locus_tag="PKM87_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HNQ28997.1"
                     /translation="MRHEVRFSGFGGQGIILSAVILGRAAVMYDRNYAVQTQVYGPEA
                     RGGASMSAVIIDDQPILYPKVTDPDIFVIMSQEGYEKYGARATKEAVMVLDSSLVQSR
                     PACRFIEIPATREAKQTLGRDIVANIIMLGALVKTTHVVGEEAIEKAILDSVPKGTEA
                     LNLKAMKLGFELAEKGKMK"
     gene            33568..34653
                     /locus_tag="PKM87_02660"
     CDS             33568..34653
                     /locus_tag="PKM87_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-CoA synthetase subunit beta"
                     /protein_id="HNQ28998.1"
                     /translation="MKLLEHEARNIFREYGIPVPAGFLIRSPGELASRMGELGERFVL
                     KAQVEVGGRGKAGGIVVADPTTGERIAGDLFRKEIKGVPVREVLAVEYLDIQKEYYAS
                     IAIDRSTKQALILFTEEGGVDIEALAKERPEAVRRASLPLLMRDIPPFMIRDLLGAAP
                     KEIGKVLNQLYRVFSEKDALLAEINPLVTTSEGIFAADAKLIVDDNALSRQGIKLNRD
                     LTDREREAEMHGFSYVELKGDIGVIGNGAGLTMATLDLIEYYDGKAANFLDVGGGADQ
                     ERVMHAVRLVAADPSVNVIVVNLLGGITRCDEVARGIISAGISQPVIVRLAGTNEAEG
                     RQILDGSGYRMMESMEQAVKAAVEAVQ"
     gene            34650..35516
                     /gene="sucD"
                     /locus_tag="PKM87_02665"
     CDS             34650..35516
                     /gene="sucD"
                     /locus_tag="PKM87_02665"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNQ28999.1"
                     /translation="MIYGDKHTGVIVTGATGKQGAFHIALMNEYARQVGGEGVVAGVT
                     PGKAGQEVAGVPVYNSIHDALKEHDASASVLFVPGFAAGDSIMEAASAGLSLVVAITE
                     HIPVHDTMRAISFARIHGCDVIGPNCPGLLSPGEIKMGIMPVHLSSRGHVGVISRSGT
                     LTYEIVDQLTRAGLGQSTIVGIGGDPVIGQTFRDVLRRFEEDDQTEAVVILGEVGGSL
                     EEEGAKATDLPIAAYIAGISAPPEKRMGHAGAIIEGGEGDARSKIERLRRMGVPVASR
                     PSEVPRLIKEIL"
     gene            35603..36457
                     /locus_tag="PKM87_02670"
     CDS             35603..36457
                     /locus_tag="PKM87_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNQ29000.1"
                     /translation="MSDHPSTGYDPMTGEEELQGPERYLRPGEELLIYTPDIQIKKFR
                     FDAYLTNTRLFLTDQDEKTPGVTAKEIPIESVADTYLEHSPVQEPILVLSIRTSDDDL
                     RTMKMTFVHNGADRLPQAEEWMHLIMHGHAKRPSGGTAVRPASPQPVPAISPEARALS
                     ETIIYPEKTTVEPGPISSAGPAVVQVEGRTRTPPPQPRQPEAPRAASTPPGAATTQIQ
                     FCFHCGHKLPPNANFCPYCGTRLHQAGAPLQSPPPIQIQRTEDEPASLASKEKEKRGL
                     FGWLFRKK"
     gene            36699..37901
                     /locus_tag="PKM87_02675"
     CDS             36699..37901
                     /locus_tag="PKM87_02675"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNQ29001.1"
                     /translation="MRRNIKIEALHQIPLERQEIELVERKCIGHPDSLADGIAESISR
                     ALSKAYLEECGAILHHNTDQGEIVAGESQPKYGGGKVIRPIYMLIDGRATKQFDGINI
                     PTDAIAVEAARTYLRETLTNLNMERDIIVDCRLGTGSTDLRDVFKPCQNTPPRANDTS
                     FGVGHAPFSETETIVKAVSDYIDGTFRPRYPAVGQDIKIMGLRDGDTITLTLGCAMVD
                     RYCSGLPEYLEYKDLLREHILNVARQHTGRKVIAAINTADDIDTGSVFLTVTGTSAEM
                     GDDGSVGRGNRCNGLITPNRPMSMEATSGKNPINHIGKIYNVISTQLAQECVAKVDGI
                     EEIYIRLLSQIGKPIDQPLVASVQILPGEGVDMKTISPDIEAIIDNGLAEITCITEKI
                     IRGEIKTF"
     gene            37879..38796
                     /gene="hisG"
                     /locus_tag="PKM87_02680"
     CDS             37879..38796
                     /gene="hisG"
                     /locus_tag="PKM87_02680"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNQ29002.1"
                     /translation="MARSRRSEGEESPGPVARPQVLRLAIPNKGRISVPVNELMEKSG
                     LHLLDVGERRLVSRTPDPHVEILFARPVDIPEYVANGAADLGITGHDMVLERKSDVSE
                     LLDLKIGSASLVLAVREDAGYTNPLDLEGTRVATEFPVISKEYFQEKGISVTLVPVGG
                     ACEATPYLGIAEAIVDLSSSGNTLRSNRLRVIDTVLPTTTMLIANPQSLASKGGKIEE
                     IVLALESVVRARGQCYLMMNAKRACVDEVTSVLPGLSGPTVMEVASRQDMVAIHAVVD
                     EERVYQLIHLLKRAGARDILVMPIERMIP"
     gene            38798..39508
                     /gene="hisA"
                     /locus_tag="PKM87_02685"
     CDS             38798..39508
                     /gene="hisA"
                     /locus_tag="PKM87_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNQ29003.1"
                     /translation="MRIFPAVDILNGQCVQLVQGRKESATVFGDPLSCAHRWLDLGAR
                     CLHVVNLDGAFGKSSANAGKIRELVRETGVEIQLGGGIRSFSDASSWLETGIERVILG
                     TLATRDPPVLKRLSDEFGSARVMAGVDARGGEITIEGWQKAGGDYLVWAERFENMSVG
                     SLLFTNVDVEGLQSGIDPGPVARLLRRTRLPVTVAGGVTTAGDIRVLRSLGASGVVLG
                     SALYSGRLHLHEAMEAAE"
     gene            39505..40086
                     /gene="hisB"
                     /locus_tag="PKM87_02690"
     CDS             39505..40086
                     /gene="hisB"
                     /locus_tag="PKM87_02690"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNQ29004.1"
                     /translation="MRCVTLTRKTKETSIDLVLDLDSPGRGEISTGIAFFDHMLTAMA
                     THGRFGLSCRAKGDLEVDVHHTIEDTGIVLGQAFRDAIGDGRGITRFSHAIIPMDESL
                     ATVALDCGGRGYLVYRGAFAHPRVGGIEREIFEHFFYSLCIHGGITAHLTMEGKNDHH
                     KCEALFKAFGIAIGAATRLGGDADHVPSTKGVL"
     gene            complement(40172..40567)
                     /locus_tag="PKM87_02695"
     CDS             complement(40172..40567)
                     /locus_tag="PKM87_02695"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNQ29005.1"
                     /translation="MMTGHLLGVAEKTSPDIVQSITELGKKGQPAVDFLLLALNDGDK
                     RVRIAAAHALGKIGDSRSVEPLISMLQDRDRDIRFISASALGMIGDPRAIEPLARACI
                     DENCFVRITAKEALTRLSSPVENNSLRFF"
     gene            complement(40803..41009)
                     /locus_tag="PKM87_02700"
     CDS             complement(40803..41009)
                     /locus_tag="PKM87_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFYB/HAP3 family transcription factor subunit"
                     /protein_id="HNQ29006.1"
                     /translation="MADLPIAAVVRIAKKNGAERVGSDAAAALVAKAEEYIANLTKEA
                     NRLALHAGRKTIKEEDVELAAKSA"
BASE COUNT         8988 a        11535 c        11725 g         8766 t
ORIGIN      
        1 ctgaggagaa ccatctgcag aaggtgtttg gacaggagta tgtgaaatat cgcaacgggg
       61 taaggcggta tatctaactg aggaaggcgg acgtattgag gagcgaggat ggtgacccaa
      121 cctcagtatt gtttcggatt tgttcgaaaa tatttcatgc cttacagtca tttcaccata
      181 aaaactccgt tacacttttt cacccgcatt tctgcgcgag cagaaaaata actgatttta
      241 gggctccgtt gccaaaccgg ctggggcttt tagagagaat atcaaaatgc cgtaattcga
      301 gagaggcctg ggccccaaca tcttgagcgt catcatgtcc cggaaaacag cgacaagcac
      361 gatgcggagc atggtgcgag gagcgggagg gtgcagccgc cggggtggag gccccgcccc
      421 ttcggtttgg tagggacagg gttgggagat agggtcgatt gcagtggcga tgaaatgcgc
      481 ggtcagaaaa aggacagcat agaacactct gttccttgtc ccacccgcag cattccggac
      541 ttttattgct tgggaattat atgggctcgc atctgactat cgcccaatgc tcgcattacg
      601 ctcgcttcgg ggcttggccg gcagcggggt gataggatgt atcggacgct tcgcttcggc
      661 gacgccctcg ctctcgctcg gtggcggact ccgcttcgct ggtatgaggg gaccggatgt
      721 gttgacggct cgacaggctt gactctcccg gccggcctgc tccgctgccg ctccgcttac
      781 ccgtccggca ggtcaaccgg tcggcctggg tgatttcggt aaaccgatgt tggcccgaag
      841 ggcatctatc atgatcactg cctctccttt tggagaggag gagaccggtc cggaggatgc
      901 ggagagaggg ggatactcgc ctatccgccg gcgtgacgag aagggcaggg gagtggcttc
      961 ctctcagatg cctgatgcga agggaaggta tcagagattt tcctggtttt ttaaagtgag
     1021 gaaaatcaag acaatttcag aaaaaccgta tcttatcagt cattatttta catttgacag
     1081 ttccgtaaga tataaaagaa caaaaaaaga tgctcatata atctaattga acgctcaggt
     1141 ggatttcagg aaatggtgat gcacgctttt ctggatcgtg actgttctgg agtggatccc
     1201 ccctgatttc tgttcttttt gtcgataatg aaccgcacat cctcgatgtg acgaaaatta
     1261 cccttgaaaa atcgggttta ttttcagtcg atatttctga atcggcatcc gccgcaatgg
     1321 aaaaattgaa gcacgcatcc tatgatgcca tagtctccga ctaccagatg ccggacatgg
     1381 atggaatcga atttctcaaa aaactccggg attccggaaa caatattccg ttcatcattt
     1441 ttaccggaaa gggacgggaa gaagtggtga tccgggcgtt cgaaaacggg gcgaacacct
     1501 atgtccagag agggggagat tcgaacgcgc agtatgcgga gttgaagcag aagatcctct
     1561 cggccgtgaa agcctaccgg gacgaaaaag cgttgaagga gagcagggag cgcttacgtg
     1621 ccctgataga gaagtccagc gacctgatca ggatactgga cagagacggt aagatcatct
     1681 ttgacacagc cgcgtcagag caggtgcttg gatatcctcc cgggtatacc cttggcagga
     1741 gccctcttga gttcattcac cccgaagatg tcgagtatgt ccgcagggaa cttttcactg
     1801 tctacgagca cacgaacacg ggtgtcccgg ccaggttccg gatacgaaga tccgatggca
     1861 cctatacgtg ggtagagtcg gtcgggcaga actacattgg cgtgccgggc cttgacggcg
     1921 tggtgatcaa cacccggttc atcgacgaca gggtagccgc cgagcaggca ctgcgggcaa
     1981 gcgaagagcg ctaccggacg ttcgtcgaga acgcctcgga aggcgtggtg gtcgtccagg
     2041 acgggctggt ggagtatgca aacccggcag cactcgggct cgtccatgtg actctcccga
     2101 atattcatgg acatcccatc tcggaattta tccaccacga tgaccagtcc atggtcaacg
     2161 aacggtacag gaaacgcatc ctgggtgaac cagttccaca tacctacgat ttgcgattga
     2221 ttggaaagga tacgagcgag atctgggtac agctctccgc ggtccggata acctggaagg
     2281 gacggccggc cacgctcaac tttctctccg acatcacccg gagaaaagcg gtagagcagg
     2341 agcttacaaa agagcgccaa cgacttttcg atgtgttgga gacgctcccc gtcatgatct
     2401 gcctcttaac acccgattat cacgtcccgt ttgccaaccg agctttccgc gagaagttcg
     2461 gggagccgca ggggcggcat tgctacgagt tctgttacgt gcagaacgaa ccgtgcagtt
     2521 tctgccaaac atacaatgtg ttcaagaccg ggaagcctca caattgggaa gtcaggttgg
     2581 aagacggaac gtatatccat gcctatgatt tccccttcac cgacacggat ggttccccgc
     2641 ttattctcga gatggacatc gatatgacgg aacagaaact gatcgaggcc gagatccgat
     2701 ccctgaacca ggtcctcgaa cagcgagtgg atgagcggac tgcccagctg actgcactat
     2761 tgaaggaacg ggaactcctg ctccgcgagg tccaccaccg ggtgaagaat aacctccaga
     2821 ttatcatctc cctcttaaac ctccagtccc ggtatctcac tgatgaaaag accctcaccg
     2881 ccattcacga gagccagaac cgtatcaggg cgatggcact ggtccatgag cggatgtacc
     2941 gatccgagga gatctcgcac atcgcgttca aggactacgt caagtacctg atctcccacc
     3001 tcttcgggtt ctatggtgtc gagacccgca gagtctccta cacggtttcg atggagggcc
     3061 tccccatcgg aatcgacacg gccatccccc ttgggctgat catgacggaa ctcgtctcca
     3121 acagcctgaa gtacgcgttt cccggcagca agaagggaac gatctcactt gaggggagtg
     3181 cgctggacga tcacacctat cggtttatcg tgaaagacaa tggcgtcggt attcccgaag
     3241 agattgactg gaggaatccc gattcacttg gccttcgcct ggtgaactcg ctggtcaccc
     3301 aactgaacgg gactatcgaa ctggaccgga cggacgggac gacgttcacg atgatggtgc
     3361 actcaaagga gaggaatggg aaaggggggt aggctatccc gctcacgcca cagcggcctg
     3421 agcaaccgga aaggatgagt tactgagcgt cactggatca cgttcaacag cgatgagaat
     3481 ctgcgacgaa cggggaagag tatctcgcgt tgaggagttc acgacgaggg gaatttcaac
     3541 gaaaagtaga atagagagat gattaaaaga atggaaaagt aagaattcat atgaataaat
     3601 acactagaaa tattggatat atcgcgatat ccgcggtatt ctttagtttc ctagtcttcc
     3661 tcatcactaa ttcgtatcct tacgaaattt tctatttcgg taaagaacga tatgatgcaa
     3721 tctctttttt tctaggtttg gtatcatgtg ggggcgtttt cgttttcctt tattccataa
     3781 aaaatgatgt ccaaatgtgg catcttggac tcccgcttct cgttacaata gttgtagtaa
     3841 ccatctcatc gcttttatca ccgatttttc cagaatattt tggatatcct atggatggca
     3901 atgcaacgcc gttcaattta ctgagtggac aaattatgcc atatcttctt gcaccgttta
     3961 gtgcactttt tttctattca ctcaaagaga tgaaaggtga tgtgagaatt ctaaccctga
     4021 tttctttcgg aattagtatt cttttctcag gaagtctctt catagagatc ccgatgtatg
     4081 acgtatctat gtcattcctc ctatattggg tatatggcat gcctctcatt ggtgccctgt
     4141 atctcgcgtc atgcacaggg tttgattatc ataaaccgca gcggcctaac tgacctgagg
     4201 taacgattca ctgagcgtca tcgtggtccc gtaaacagcg aggagcagtc tgcgatagca
     4261 gtctgcgacg agggggagag tgcagcctcc ggcgaggagt tcacgacgaa gccgggggcg
     4321 tcagaggacc aggacaaaga agagaagcag gggcatggtg cgagtgcagc ggagtggagg
     4381 agcaccggac caaagggagt agcggacaaa gaaggagcga aaggagcaag gtgccccgtt
     4441 gttgtgtata tctttggtcc cctccctcat gcgacgaggt tctgaccgga gggaggaacc
     4501 gcacgggcga aggagcccgt gccgaaggag gaagggcggg gttggggaaa ggggcggtcg
     4561 caggggcgag aatgtgggcg gtgggaaaaa gaaaacggaa ttgaactcca tgcccccttt
     4621 cctcccccaa aggctctggg catcaagttc gattctcctg tctgtttttt taaaaaaaaa
     4681 tcaaaggtaa cgctttaacg cattgcgctg ttcaggatac ttccctggag agtgaaaacc
     4741 gttcacgtcc attcttcacg accgtacgaa cggagtagat atcccttatc ctgtcatcgc
     4801 aaaggtggta acgtgtgcag gaatgtgaga gtgtgaagtc tggcgtccac ccctcgtttt
     4861 ttcctgtctg aaggtaaacc cactctgtat gacagggcaa ggaccggatg cctgtctcga
     4921 aggaattctc gcactttccg gggacgaccg gtggaagatt gaccactcgc acgcagtcat
     4981 caatgtcacg gcgtacgtcg tccggtcatt tgatggtaat gccctcatct ttgccatgcc
     5041 attgcatgtg ctccgtcctc tcctttcaga ggttccgctc gacctccggg ggtcacccgg
     5101 tgcggttgcc tgcatcatcg agcaaatcaa gcagagggca cagtcggatc cggcactggg
     5161 gccgcttgtg ggacggggca cccggtttcc ccaccagttt gcctcaatca ccgagcccta
     5221 cacggttgtt ggatcttcag ctgcactggc atcagatctc tggcatgcag gggaccggaa
     5281 ctttgcagat gcgagcgggg ttcacctcct cctgaacggg gcagtcccct gtccgcaaca
     5341 gttcacccag gctgatgtcg catcggtcat agaaacggtc gcacgcctct gcgacgcggt
     5401 cacggcaatt gcgtgttttg tcccggttcg cgaactggaa accgcctgga tctcgacact
     5461 cgaccagcag ctgctcagag agatgctccc atctttggga cttgtctctt tcattggtga
     5521 tggagcacgg cttgcacgcc actacacgcg gtatcggtgc tacttccgga cggccgggcc
     5581 aaagaccgga gttcacatac cgtttgcctg tcccctggaa ctggacccgt gcgaactgga
     5641 actccccgct agcaaccgga caatcacagg tctggggatc cgcagacgtg aggtgtttgc
     5701 tgttgccggg tcaaacgcac agggcaagac gacgtttctt gagggtatcc atgccggaat
     5761 ggacgaccat gcgacaggtg acggcaggga acttgcggtc acagtccctg gactctgcac
     5821 cgccgaagca atgaactgca tgctgaccgg ggctgacgtg agcatgttct tttccgcact
     5881 tcctccaggc atcagcggga cggcacatgc cgcgtcggga atgggcagcg ggtcgatgaa
     5941 catggcgtac caggtgcaga gggccattgg gagggattgc ccgcttcttg ttatcgacga
     6001 ggaccgggcc gcaccgaacc tgctggtccg aagctgcctt cagactcatg agatcacgcc
     6061 cctttccgag atcttaggcc acgaccgtgg aaagatggga gagaccgccc tgatatttgc
     6121 cgcatgtgcg atggacgtgc tggtagccca ggcagaccgg atcatgctcc tcgacaacca
     6181 taccgcgtat gccgtcgatc gtgaagtgtt caggaagagg gtggcagagt ccctggaaaa
     6241 gatagcagga gatctgcggt agtgggtttt ttcaggggag aaaagattat ggattcttct
     6301 ctgcggatgg tcctgttgaa tccctggaat aaaagcagcc atacacattt ttcttgtgta
     6361 tctccaggtt ttttgttttc ccgcgatagt ccgggcagca taattcctgc acactgggat
     6421 tgacattctg cgataccagt ggtattctca tacggaaaag tcagtattcc tgagaaattt
     6481 cattcccgcc ggagggtagg ctgaaaaggg tcaagggtga tcaccttttt tgtattcttc
     6541 cgctgaatga tgcatcatcc aggcatgggg acatgacacg caggccaaaa tatcccatgg
     6601 atgcgtgaac ctccgcctta tttccggaga aggagagatg gagccgtccg ttgaatacat
     6661 actcctcgtc atcgctgtgc tgatccttgt cagcatcctc gccaacaagg tatctggaag
     6721 gctcggtgtg cctgccctcc tgatcttcct cgtcgtgggg atgctcgcgg gatcggaggg
     6781 gccgggaggg atctattttg acaacccctg gatcgcccag gcaatcgggg tgatagcact
     6841 cgcgtatatc ctcttttcgg gaggcctcga tacccgctgg tcggaagcca ggcctgtgct
     6901 cccccaggca gtcgtgctct cgaccgcggg cgtcctcctc accgcgcttc tcatggccgc
     6961 catcgcggtg gtggtgttca aagtcaccct gctcgaaggg ttcctcctgg gggcggtcgt
     7021 ctcctccacc gatgctgccg cggtcttctc gatactcagg acaaagaggg cagggctgaa
     7081 gggaatcctt cggccgctcc tcgaactcga gtccgcgagc aatgacccga tggcggtctt
     7141 tctcaccatc ggggccatca cgctcatcct gaacccaggg atgccggtac tcgccatggt
     7201 cccgctcttc atccagcaga tggcagtcgg cgggctcatc gggtacggga tggggagggt
     7261 cgtggtctac ctgatcaacc ggctcaagct cgagttcgag ggattatacc ccgtgctcac
     7321 cctttcgctc gtcatgatga cattcggcct gacggcgtcg cttgggggaa gcgggttcct
     7381 cgcggtctac atcgcagggc tcgtgcttgg gaacagctcg ttcatccaca agaacagtct
     7441 gatgcggttc cacgagggta tcgcctggct gatgcagatc gcgatgttcc tcaccctcgg
     7501 tctgctcgta ttcccctcgc acctcattgc agttgcaggg gccgggatcc tcttctcgtt
     7561 cttcctcata ttcattgcga ggcctctctc tgtcttcctc gtgctcagcc cgtggaaaat
     7621 agccctccgc gacaaggtga tggtctcctg ggtgggtctc cggggggccg cccccattgt
     7681 ccttgccacg ttccccctcc tcgcggggat tccccaggcc gggacgatat tcaacctggt
     7741 cttcttcatc gtgatcacgt cggcgctact tcacggaacg tctatcccgt tcatcgcacg
     7801 ccggctgggc gtatcctccc ccctgcagga ttccccgaaa ctcgcaatga gccttgtggc
     7861 agggtgcgat tccgggaacg acctcgtcga gatcgtggtg tcaccgggtg cagtggttgc
     7921 aggaaagcag atcgtcgaga tcgggctgcc ccctgagacc ctcatcatca tgatggagaa
     7981 aagcggaagg cggtttgtgc cctgcgggag caccatcata gaggcggggg acaccctcct
     8041 cattcttacc agccctgaac ttgggggcag ggtccgttcc ctgttctcgg ccgcgcaccc
     8101 cgggaccggg gtcacggcca gggaaacgga tgaagagaaa gatacgatat gagtcgttcg
     8161 cacggacgtc gatttcgggg gaacactggg cactcactct tccctttcac aaagacagca
     8221 gggaactcag gggttgcatg cacgtcaggc cctgccgagg ccgggaggtt cttccaggtt
     8281 attcgggctc gccaagatcc cttgctcttc tggaccggca gactggttcg tctcctttgg
     8341 gacaccgatc gtgatccggt caccctcacc cgcatctttt catatatcga tagagaggat
     8401 gcactgatat caaaaaacct ggagaaacaa cagctcacaa tatgggttcc tatcgcaggg
     8461 ccagttcaat tccgacccga aggtcatcat cactgaaggg cttcaggatg aacccgttag
     8521 gatttgcctt ctttgccctg tcgatgatct ctccttgtga atatccggtc acaaatacaa
     8581 tcggtaaatc atgggttctg cggatttcat gggcaaccga gatgccgtca caattcccgg
     8641 gaagcccgat atcaaggagt acgaggtccg gagacatgct ctggatgaga gatattgctt
     8701 cctttccttc tgctgcccgc ccgcatacat cgtacccgag tttcttcacc cgccactcca
     8761 ccagtctggc gatgacgtca tcatcttcaa caatgaatat gcgacttttt gacattgggc
     8821 cgatttatct ctccctttta ataaaaactg cttcaattac gcttataaaa atttgccatg
     8881 cactcgttca tcggagcaat gaatattcat cggtgcgatg aacgaaccat taaaattatc
     8941 gccgattgcg atagcccggc aatctccatc tctcaggaat ttcgcagcat atcagagttt
     9001 actgtctcaa tacaatagag gtgcagcggc cagaaatact gaatgcatta cagtattcac
     9061 ctgttaccgg aatagtccag gtgaggaaag ccctttctct gagaaacaga gattgtatag
     9121 catgaagtac gagatcaagt acaaaccctc ttattcgctt cttgtggtca ccctggcccc
     9181 aggcgagagc atcgcagcag agtcggggtc gatgacctac atgcagccca cgatcgaggt
     9241 gaagacccag aaaagggaga agagtttctg gggctcactg ggccttgcac tcgtgggcgg
     9301 gcagagcttc ttcatcaacg agttcaccgc cacgacagga accggcgagg cagggttcag
     9361 cgccgcccct ctcggtgaca tcgacaccat tgcggtcagc caggggaaag gattcatcct
     9421 gcagaaatcg gcatacatcg cgtcccaggg gaccgtgaac cttgacgtca agtgggaagg
     9481 gttcacgaaa gggctcttcg ggcagggcct cttcatgatc cgggcgacgg gcgaggggac
     9541 catcttcatc aacacgttcg gggcaattga ccgtcataca ttaaaggacg gggaaaaact
     9601 tgtcgtggac aatttccacc tggtggcatt cagcgacacc tgccagtata aagtgacgaa
     9661 gttcggcggg ctgaaggaga cgatactggg cggagaaggg cttgtcaccg aaatccaggg
     9721 gcccggcgag gtcttcatcc agaccaagaa catcagggaa cttgccgact ggatttggac
     9781 aatcatcgag ccgagagtgc aggccaaggc aaggtgaaac actccccact ttggccgggt
     9841 cggatcccga tcaaccgttc ccggggaaag taaatctctc ctgaagaagg aacccgtttc
     9901 agtggtgccc gaatccttct gcgaggggga caactaaata ccaaaggaca agataactgg
     9961 catggaatct caacacgagg gaagagtacc agatggaacc ggctggattc gttcgccgct
    10021 gaaacttctc gccgcggtca tcatatgcaa tatggcggga atcatcgggt ctttcgtgac
    10081 ggtcacggga gaagggtcct ggtatgctgc catccagaaa cccccgttca atccgcctgg
    10141 ttttgtcttc gccccggtgt ggaccaccct ctttgtcctg atgggcatct cgctttacct
    10201 tatctggatg gaagggatgg agaggaggga ggtccgggtc gcaattgccc ttttcgcggt
    10261 ccagcttata ctcaacacga tctggtcctt cctcttcttt ggcctccagt ctccattcct
    10321 tggccttgtc gagattatca ttttgtggat cttcatcgtc gcaaccaccg ttgcctttta
    10381 ccgcatcaat aaaaaagcgg catacctgat ggtgccgtat ctcgcctggg tcacatttgc
    10441 ggcattcctc acctacaata tctggctgtt gaatggatga agggatccaa aattcttttc
    10501 acatggtgca agaaacgggc tggccaggac ccggctcctg cgttctgaat tgatatatcc
    10561 ggaatagcaa taccagttgt cccgctttta ttgacagggt atgctgatcc aaataactgg
    10621 tgaaaaagag gataaaatca aggataatgc acggttttgt acaggatcgg tttttggtaa
    10681 gacctgaact tgcttgtgga tactggtttt tggatcgccg aatatccact gatcacgtca
    10741 agggtgaact tctgttcatc gcccgttgtc ggcatccatg actgcttgta gatggccgaa
    10801 atggtatggg ttcctgtccc ggtcacgaga aacttccatg tccgcatccc cgctgccccg
    10861 attaatcccg tgctggatgg ggagtatgta tcccgcaggg tctgtaatcc gccactcgtc
    10921 tccattatcc atcgaaaacc ggtactcggg ttctctttta aggagagggt gatggtatca
    10981 ccagggatga cattcacgga atctccattg ttttccaggg agaattggta ggtggctgcc
    11041 atgaccggtg ccgacgcaca tgcaaggacc gcaagcgctg acaggagaat tattctgtgt
    11101 atttgcattg gtgaacacac ctctcgctgt gagaaatatc gtcgcgttcg tatataaaag
    11161 gctccacgct atcaggtgcg ggtaagagtg tttgaccgga ttcattctgt ggagcatgta
    11221 ttctccctgc atttccggta tgcaatccat cgcgtcaatt atggaattac accaaaagct
    11281 tccattttta aaccgaaagg ggcactttgg ccaccaatgg tgcatttggc gaggtgaaac
    11341 tgatttcggg accataaaac ggattgtaga gaagggggga aggtggtttg gtgatcggac
    11401 ggggatgcag atcccaacct ctggtccgcc tcccccatat acgagtgcaa ggagtatgta
    11461 aatggggcca cacatgcaga gggttcctga tggaagcgca tgggctggga gggcaggtga
    11521 agggtgtacg tcttatttat ttccatccct ggaagagtgg gaggattatc tcgaagatga
    11581 gcgtggcgat gatataaaag accgacgaga ttcccatcgc gtagataaaa aagatcgccg
    11641 gggtatacac gagcgggacg ctgcgcaggt tgatcgcaag gagggatgca agagacacaa
    11701 cgaatacgac cagagcgtag gtcatcgtcg cgaggacaaa cctccggatc cgggtattcc
    11761 tgctgacaag gaccagtatg gaggagaaca ccacaatccc gaggcagaac ccgatcagcg
    11821 agaagatgac tgccatgacc tgcgtgtgga cgagattgat ccacgtggtc agcgtcagca
    11881 tcagcaccac cagtgggatc acaagaagaa ggcgcttgaa cgcctccacc ccaatgaaat
    11941 agtaaaagat aggcctgtcc ctgggctcgg cgaatgccgc ccatagcagt gcgaacacac
    12001 cgaggtaggt gatcagcagg caggatgcga tgctgatctc ctgaaggtca aagggaatgc
    12061 caagcagctc gaatatcggt tccctgctcc catgacctga gaacgtctgg gtgagaaagg
    12121 cggtgagtgc ggtaaaaatc gagacgaaga tgaagagaaa ccagtacgcc cttaaaaacg
    12181 agctcagctg gaattctttc tctcccacct cgataaccgg taaaggcacc atgaaatggc
    12241 agagtgtcgc tgtaaaatca catatatctt tcttcatgct cgccggattg atatttgcgc
    12301 ggaagatgta tggccatttc cgcctgattt aaatagtacc cgggtcatct tcctgctgat
    12361 gtatcctacg gcactggacg ggggcgtggc atgatatcaa tactcctggt agatgacgaa
    12421 gtgcagttcc tggaggtgac tcgcatgttc cttgagaagg gccggggcat ccgggtgacg
    12481 accgcaacgt caggagacca ggcactcgag gtcctcgcgc accataaatt tgatgccatc
    12541 atttcggatt atgcaatgcc gggaatgaat gggatcgagt tcatgaagca ggtgaaggcc
    12601 ctgggtgacg acacgccctt catcatcttc accggaaagg gcagggagga cgtggttatc
    12661 gacgcgttga attttggtgc ggacctctac cttgaaaaga gcccagaccc ccggacacag
    12721 ttctcggaga tgatgcagaa ggtccggaat attgtcacaa ttcgggcgga tgtccaggtg
    12781 gcacgggaga agatgcgcat attctcttcg gtgatggaac ggtttcctgg gatgatcttt
    12841 cgcatgaaac tcaacggcag gtgggcattt ctctttgcga gcgccggtgc ggaccgcctg
    12901 accgggtact cgggtgatac acttgtgaag ggtgacccgg cattcgagat gatcgtaata
    12961 cctgacgaca gggaacgggt cgatcagtca cggagatctg ctctcgattc ggggggtgaa
    13021 taccaggtga agtaccggat acgcaccagt ttgggagagg agaagagggt tgtcgaaggg
    13081 ggttttgttg tcccgggttc ggatgaagag atactggaag gatatatcct cgaccttgat
    13141 atcgtaggaa acgggtcagt ccggtagggc tcacaaccac ccgttacaga ttggacccat
    13201 ctcccttgct attgcttttg tgcctgccgg aaacaccctt gcggaatgcg gatctcgaac
    13261 ctggcccctt tcccaaatgt ccccgtttct tttattgtaa gaccggtgat ggccaggatc
    13321 tcgcgggcga ggaaaagtcc gaaacctgta tttttaccgt atccacgttt gaatatctct
    13381 tctttctcgc gtgaaggaat tccctggcca ttgtcctcgt atacgatctt cattccctgt
    13441 ccatcatgga gtaccgtcag ccagatggag tctgcaccct gcccgtgccg aagagagttg
    13501 tcaatgaggt tctggatgac gttttcaagg agtggatctg cataaacctc gatatccccg
    13561 atatcgctct tcactgcaac atccttcatc gtcgcagagg ctgatgcccg gccgatgatc
    13621 tcgccgagtg actgccattg ggggacctcg atcccgatat cctggtatac ctttgtaaac
    13681 tcgatctgcc tcctgatggt ctgggtcagc tgctctacct ttgacagggt ctcctcctgc
    13741 gcatcgtctt ttggatcttc cttcaagagt tcgatataca ggagaagggc agtgaccgta
    13801 ttgaggatgt cgtgcctcgt aatgatattc aggagggtga gttttgcatt catcctgcga
    13861 agcgactcct cagccatgtg cctgctggtc acatcgtgtg ccgaaccgag gacaacggtc
    13921 tttccgccga tcaccacagg atggttcctg atctcggcaa ccacgcggct cccgtctttt
    13981 ttgtgtatca cgaattcctc gggaccggtg ggtgtgcctt cgacgtgccg ggcaaggagt
    14041 ccgatggctt ttgggacctc ttcaggggag aggatacctg cctcgaggaa gtttttcccg
    14101 atgagatctc tccttggata gccgatcagg ttctcggccg caaagtttcc atccacgaat
    14161 gtcccccgga gatcagtaag gaaatacgca tagggggcat attcaaagag cagtctcagc
    14221 cgctcctctg acgcggcttt ctgcccttcc attatcacct tgtcggtgat gtcctgccca
    14281 atgcaggaaa tccctgtcac gtttccttca aggtcgcaga gaagtgtatt gttcaggtcg
    14341 atctttcgta cctttccttc ccttgtcctg atgctgcacg tgaaatagag cgcgttcagg
    14401 tccccatcca tcgcggagag gaagaactgc tcaaccatgc gctgttcgtt gtcggggatg
    14461 aatgtctctg tccaggactt acccaccagg tccgccttct cgtatccagt gagggtcagg
    14521 agaaacgcat tgcaaaaact gatcgaaagg ttcctgtcca gctggaaggc gataaggcgt
    14581 gcattctcga gcgattcacg aaacctcctc tcgctctcga gaagttcctt cctggcctcg
    14641 tgtctgcgca cagcctccct tatcatatag agaagctcga ggaagttaag cctgggatct
    14701 cccctcttct ggaggtaata atcagcaccg taattgagcg ccttgatgac agtctcctcc
    14761 tggcctttgc cagtgaatac gatgatggga gtgtcattac cccggccctt gatcatcctc
    14821 aggaaatcga tgccattcat gccgggcaat tcgtaatcaa ggacgattgc atcgtaatga
    14881 gtggtgttca gggcgtcgag ggcctcggtg ccggacatgc acgtgtctat ccgcatctcc
    14941 tcttccttct cgaggaaacg gcatatgaga tcaagcagat cggggtcgtc atcgaccagg
    15001 aggatttgca tcatctcttc tatctccggc aaggaaccgt ttcagcagcg cgtggaaccg
    15061 cgcattccag gatgatagtc atggcacctg gagtcttttt atccttccgc tcctttatgg
    15121 tggtatccgc gatgaaattg caggagaaaa aaatccatgc ctggcgtggg aacgcatctc
    15181 cgtttcgcac acagaaaagg gtgtcactcc ctgacgggag tttaaaagag ttccgtgcat
    15241 ccccggggaa aaaagactgg acttcgcttc ccactgtacg gccgaaagga tcaacgggat
    15301 catcctcgat aacgattgca ggatcacgct gcccggccgg cattgtattc gcggacaatg
    15361 atcccctcgc ggtccccaga gaggagcgcg tggaggtccc tgcaggagac cacgaaaagc
    15421 ctgtcatgga taagggcagt caaatcctgt ttctttctcg cgagcaccgg ggagagccac
    15481 gcgattcctt cctgttcacc ctcgtcattg agtatcatgg cgttccggtc tgaaaatagt
    15541 ccacctacct cttcgggggg gacatagaag atgcggtccc caagggaaag ggacatcccc
    15601 tttccagaac gccgggccgt gcccgcccgg gtaaagacag ataccggtgc ctgcctcatg
    15661 atgtcaccgg ttattctgtt gttcttcttc acatgtaaac ctggcgtcag acgacaaatt
    15721 gggttcagat tgatgaataa acgttaaaat gcaggattca aacttcacag atgcggggtc
    15781 ggatctgcgt gtgcgccatg cgaaaagacc gctttgggaa tcggtggcgt cagcaagtgg
    15841 cacataatcc gtcagacatt tgtctgacag attgtgcgct tctcagacaa aatgcctgca
    15901 tttttgcctc aagtagagat tgtaaagtga tttgcgatta ttttccaaaa agtttatatg
    15961 gaggacaata caacaaaaaa tgtgaaacca acatggttca gtgtcagtta atgctgatac
    16021 tccaattgat cgttgcctga tactctcacg catcaattga agaaccactg cctggaccat
    16081 ggtggtcatt tcctttgagt tctccttctg ccaatcaata tctccttaag aaaagtcgta
    16141 actccggtca tccgcgttat tttcgttttc agccaacgaa tgtcagccaa tcatgacata
    16201 gacaaggatg gagaggagga agaccaggag gagcattcct gcactcagta ccgggatgaa
    16261 gaggagaatg gtattgacgg tgctcgccat ctgggtgatc cgatgggaac cgaataccac
    16321 gaccccctcg caccaggcag tgctccccgc ggtttctgca ggggccatgt ctgcgagggc
    16381 ctggcgaagc gccgcatcca cacacgggat gatctcggtg accgggacat ggaggccgac
    16441 ggggttcttt ccgctcaccg tattgaccac gtgggaatcg gtcgtcatca cttcggcctc
    16501 gtcaactgat tcgagtaaaa aatcccgtat ggtctccctt accccgctct ccatgttgtt
    16561 tccgtcaatc agaaggtagg cggtgctctg ttctcctgcc cgcacaacag cgagctggat
    16621 gccctggtct ccaaatccct gttcgcggga gaaggggacc tgcacatgcg cgatgccaag
    16681 ctccagcgaa aacctcggca tcccgggtgt ctcgtcaatc gcttttatcc ccgcatggta
    16741 atactccatc gcaagcctgg tcgcggggag gacataggtg atatccccgg catagcaatt
    16801 gtgggcatcg atgaacgcga tgtgcgggaa ggaccggtgc ccctcggcca tgatggtcat
    16861 tccgatgttg aagtcaaggt cctcggtccg ttcgggtgac ctggtactta ccatcaggag
    16921 agtatcgcga aagacctggc agaggagtga tactgatccc tcctggtacc ggaccgaacg
    16981 ggttgcggaa ctagaaaaga cgaggttgtc cctggatgcg gccactgcct gcacgagctt
    17041 gtcaatctct tgctcagata caaggttgaa gtcatgagtg gcggccccgt gcgggaccat
    17101 caccatctcg gagaatcccg actgcatccc ccttgggagg tttccgcccc cgatctcccc
    17161 catgggcccg gggtgcacgt tcgggacagt gaggaggagg ccagtctttt cgttcctgcg
    17221 aaagaatagg gtgacctgcg gcacggtcac ctcctccccg atcttgcgga agaagtcctc
    17281 gagggctttt gacccgtctg tcaggtgcgc aaggaacgag ttgagaaaat ccaggcccct
    17341 gatttggaat gtccggtaaa ggggcctgtc gattgcccac acaagcagga tgaagccgag
    17401 gccgaagaac acctggagga caagtgcaag gataaaaaaa ttcccaggaa aaaggaccat
    17461 tcccatggca atccccgcca ggctctggct tgctgccggg agaatcatcc ggaggacccg
    17521 gtaatccgcg attgcaacca ggacgagaag ccggaggccc aggattatgc ccagtgagat
    17581 agcgtaaaag agggggagca tcgactggga gatcgtcagg gatgagaggg tgatgatcac
    17641 cccgaagagg gtacaggaga gcgccaggag cgcggacctg ttccaggtca tgggacgccc
    17701 gatcagttcc accgcgggct tcgtcacaag gaatgcgaag agggccggga tggtgaaggc
    17761 acgcgtcccg agccagagcg gggatccgag tttcaggctg accccatcca ggatgaaccc
    17821 gagcagcagg agaagggcaa gcgagattgg ccaggacggg gcattgaaga ggtagcgccc
    17881 caaccgctcg atcttcaact ccccccccat cactgacatg tccactcaca cccgggaacg
    17941 ctgagcttct cctgccccgg gctcaatcac tggaacatcc ccgaatacct gccggaacct
    18001 cgtctccatc tcctcccatg aggggaggtt gttctggcac ccggtgactt cgacggcaaa
    18061 agaggcggtc accgtcccga tacggcagca atcccggggg gagtatcccc gcctgaacgc
    18121 cgcgaggaac cctgcacggt aggcatcccc cgcgccagta ggatctgcca tcctgacgcg
    18181 tatcgcgggg atatgggagc actcttctcc atggaagtac tcgcttcctc ctgcccccat
    18241 cgtgaagacc gccagaggga cgcgggcggc gaggccctcc cttgagaggc cgagagtccg
    18301 gcacatgctc tccacttcgt gctggttcgc aaagaggata ttgatgtggg aaagaatggc
    18361 gtccagctgg gtacggctgt acctgtgcag gtcctgcccc gggtcgaacg tggagaaggc
    18421 tgccttctct gcgacgcggg tgttgaagtc cggatcggcc gtggccatgt ggacgaaagg
    18481 gagggcaggg gcctctgagt cggaaaagat gcgggacgct ccccattcaa agaacgtgat
    18541 ctggtcccca tcctcgtccg tgaacatgaa cgcggtggca gtgtgggcat cgggaacctc
    18601 gaagaaccgc tggttcaaac caagcgattt ctgccatcga tcatactcgc tgccgggaaa
    18661 atctccccca atcgcgctca cgagggtaca gggctcgccg agcctggcga tgccagcggc
    18721 aatattggct gctcccccgc cgaagtatac cttccgctcg gtaatggtgg cagacccgtt
    18781 cctgggcggg aaagcgggga ccctgcagat gtggtcgatg gcggtatggc cgacgacgta
    18841 tatcacagtg cgcagacctc cttgacttcc agggggacgt cgcggagggc ttttccaatc
    18901 acggatctcg cgatgcgggt ggcgtgttcg ggcgactcgg cacgaaagac tttcatctcg
    18961 aggaggagcc cgacaagggc ggtgcgcgct accaccagcg cactgttcag ttcaccttcg
    19021 cagaacgggc aggcaagcat gcccacctcc acctccacgt aggctgctac cgggttcagc
    19081 ctctttcctg cctcgctgat ggcaatgctc accgcatcct cgagcgatga gacatccttc
    19141 accacccatg cgccttcaag tattacacga taatctggca ttcctggtcc ctcaccataa
    19201 ctggtggtgc acgtgttcaa ccaccggcat tcgttctgta aagctcccgg gcgaaaagcc
    19261 cttccctacg cagaggattg cgatcggccg gacatggggc ggaagatcga gcatatcccg
    19321 cacctcgtcg tcctggaacg agccgaccca gcacgtatgg agccttcttg catgagccgc
    19381 gagcatcatg taggtgcagg caatggtcgc gtcctggatg gcaaagagga cacccctgtc
    19441 gccatatctt gacatggatc tcacgtaatt cgcacatact acaaggagca gggcggcaga
    19501 tacaacatgc tcctggccga aagaggcctc tgccaccctc tctttctcct cctgcccgtc
    19561 cacgatgaca acgtcccagg cctcgaggtt tcccgcactt ggggcggtgc ttgcgcagtc
    19621 caggatatag gtgaggtcgg cgtcgggcac ttcgcgtgtg tcgtattccc tcaccgagca
    19681 gcgcgacaca aggaattgaa ggaattcaga tgagtccatg tgcttcgaga acctgccacg
    19741 acccctgggc tgcagtagtg actgcactga tggactcgct tattttcagg gacgccgccc
    19801 cgtaattctc gctctcaacc gtgctgatcg attccgagag gaggttgatg gcacgccgga
    19861 agtccgacat ctcggtccct ttccaggcaa attcaagttc tgaacggacg gcttcaagga
    19921 ggagtacgag catccgtttt ccgccggcgc gttctgattc agggaaccct gcgagtgcga
    19981 tggtgagctg cgaggagata atcagttcac tcttcgcccg ttcggcgaac tggtaacctt
    20041 tcaccgcttt cttcagatcc atactttcac tgtactgcag ggatacaaga tacttctgtg
    20101 atctaagtca aatggccaaa aaaaaattga gaatcggaga tatattgtgg ttatctgcca
    20161 gatttctgct tggcgccgag caccgagacc ttgggcctcc gcatggcttt cctgaccatg
    20221 gggtttgcct gcggaccgga ctctgaagag cgcccgccgc gcccgccgcg tcctccacgg
    20281 ccaccgcgtc ctccacggcc accgcggaaa cctccctgcc cttcgccggt caactgctcg
    20341 agcttccgga gcgaggctcc cgccttgaac cgctggttcg gccccggttt cttcaattca
    20401 ccctcagact ttggcaccag gcgtatctgc cccttaacgc ctttcacctg cgtccattgt
    20461 acggatcctg tttttcccat aggcaaactc cttaatttgt ttgcgatgtg gtcttttaat
    20521 actttggacc tctcccgtgc ggaatgagag gtcacctgcc tgaaagtgcc tggtctagcg
    20581 cggctttcaa cacctgggag acttttttgc cgtccaccgc gccccccact tccttcatca
    20641 tcactcccat caggggacca agggcgccca tgcccttctc actcacaaac gtcatcctct
    20701 cggatacgat gcgtcttgcg agggcgtcga ggtcggtccc tgcaaccgtc gcctcgacag
    20761 cctgcatggc tgtctctgcg gattctccct ggacgatcct tctgaggacg tcggggatcg
    20821 cctccttgcc cactcgttct tccacgaccc ctttcagcac ctgcaggact gccggcatca
    20881 ccgcgttgcc agccgcatcg caatcggcca cgagtgggcg gatatcgagc ccatcccgcg
    20941 agagttcctt caaggttgca atgagagtcc tggcagagag cgtggcgggg atgccgagcc
    21001 ccacagacct ctcatatagg gggaggtgct cggagtatgc cacctgcttt gccaccgccg
    21061 catcgaggcc atgcgccgtc atatacctct ccgcctttgc ctgcaggagc tcgacacggg
    21121 ggatatcctc ccagtattca ggggagattg taacggggag cacgtcggtc tcggggtaca
    21181 tcctcgcggc cccgggcagc ggccgcatgt acgaggtgct tcccccctca agcatctttc
    21241 tcgtctcttc ggggaccccg agaagggcca gttccgccct tcggatgacc tgctggcagg
    21301 cgcacccggc ctgtcggggg gttcctgcca cgatgacgat gcagtccgac tctcctgcac
    21361 cgaccatatc ccgaagcgcg gtcacttctt ctgcgttcac gccgtaggca ggaagctcgt
    21421 cggtgtggaa gatgccaccc accccgcact tctttgcata gtccgagagc tcgctcccaa
    21481 gcctcctggc tggctggatc tcatccccga caaggccggc aaacccggcg agggtgacgg
    21541 ccatgatccg ggaggccttc ttcaggatcc ctgacccagt gtgggaaaag atctccgtga
    21601 catccacagg agtccccgaa acagaagcgc cgcgttcttt caggcggtcc ctgatggaga
    21661 ggagccgctc ctgcctctcc acttccctcc gaaccacctc tgcgatgagg tccagttcct
    21721 ggacgccttt gatctctacg cgggcgccgc cgctgatcga gatgttgata tcctgccgga
    21781 tcgtccccag gccccgcttc actttccccg ttgagcggag caccattccc aggtactcgg
    21841 caatctcctg cacctcttcg ggcgtgtgca tgcagggtga agtggtaatc tccaccaggg
    21901 ggataccgag gcggtcaagg gagaagacct cgtcctttac ccgctgggca gcctcctcct
    21961 ctatgcagat cgtctctatg gtccccccgt tcggaagcgt tccgttcacg gcaaccagtg
    22021 cagtccgctg gaagccgctg gtgttggagc cgtcgatgac gagcttccgc atagtgtgca
    22081 cctgtggtat cggggccatc ccgaacatct ttgcaatctg gaggcaggtt gcgagtgcct
    22141 cggggttgag gggtgcgggc ggctcctcgt cattctctac gaggcacgtg ctgtcgtagg
    22201 tgtaatagag aaaccgccgg aaattcttca tctcttcgcg cgccgcccgg tcaatctccc
    22261 cgagttcgct ctcggtcgca cgtaggtaac gcgagaactc gccgtcatgc tcctcgaacg
    22321 gtcgaagcaa agtcgggcac ctgcagaaga gtttctccct cgtgttcagc tgctggtgga
    22381 tctcgatccc ggcaacgaga ccaagggcat tataatccat gcagcgacct ccgcgtatac
    22441 tcgcctttct ggttctcctg catcatgcag cgtgctctct cagcatccgg ctcgttcccg
    22501 agcacccaca tcatctttac aagcgctgtc tcgggaagca tgtcctcccc ctcaaccacg
    22561 cctatcctga tcaggtctct ccctgtgtcg tagacgcggt cacacaccct gccgtggaga
    22621 cactgggatg tcatcacgac gagcgtgccc tggtcgatca gctcccgtat cgcggggatg
    22681 agggaggtgc tgacgtggcc gagcccgctc ccggcaatca cgagcccttt tacatccgca
    22741 cacgagctga tcaggtcagg tgacatcccg gggaaaaatg agatgagacc gcacgacggt
    22801 tcgagccggt catggagcgc aggctcgcag gcacccctcc tcaccgcacc tggggagagt
    22861 ttcacttcga gcgacgggta gtccacgaat cccaggggag gggccccgat gctctggaac
    22921 gcatcgcgcc gtgaggtgtg cattttcctg accctggtcc cgcggtggat ggcgcagcgg
    22981 tcatcgctcg tagtctcgtg catcactact gccacctccc cgaggtctcc gaccgcagtg
    23041 ctcgcactgc agagcgcatt catcacgttg tccgagctgg ggcggtccgc cgatctctgc
    23101 gagcctacga agaccaccgg cacgggagta tcgagcatga agctcagcgc ggccgccgaa
    23161 taggccatgg tgtcggtgcc gtgggtgacg atgacccccc gtgccccgtc ctgtatctcc
    23221 tcgtacacgg cccttgccag ttcctgccaa atcgaaggag tcatgttctc ggagaggatg
    23281 gtgtagagct tccttgtccg gcacctggcg atcgcgctga gtcccgggat cgcgcgaagg
    23341 atgtccgcgg catcgaactg gctcgtcacc gccccggtcc ggtaatctat cctgcttgcg
    23401 atcgttcccc ctgtggagac gatcgagatg aggggaagcg acggatcctg ctcaacctcg
    23461 gctctctcaa ccctcagggg agaagggcgc tctatgagcg tgcagtcccc gggaaaaacc
    23521 cctatattgt acccgctgtc catcttcagg accgccatgc cgtcccggtc ggtaatatag
    23581 gtccctctcc gcccggtgcc gccgacagtg caattcacga ggtcgccggg aagaaagccg
    23641 ctcatttccc tacctctttc ttcgcatcgc tgataaggcg gcggacggat tcagcataat
    23701 gcgccattct ttcttcgagt gaccggagat cttcgtggag tgcagtcctc ctcgaagaga
    23761 gagagatcct gactgcgaac ggcgagggac ttccaggagc cctccgccgt tcgatgcagg
    23821 cccgcacgtc gagcgctgcg gcaatttctt cctccctgat ccccatcttc gaggggggct
    23881 gaattcccat ctcctcggcc gcctggtcaa gtgcagggat atcgagagcc cccttctgga
    23941 tggcctttcc cacaagggta tgcgccgtcc gaaaggcgaa cccgtgcgac tggaccagca
    24001 tgtctgcgac gtccgttgcg gtggagaagc cttttccggc ttcgcctgcc atcacggcag
    24061 tattgaatgt ggcagaagag agcatgtcga tgaggagccg ggtgctgtgc tttgcgtccc
    24121 gtatccctct ccagaggtgt gggttgagtt cctgcaggtc tcggttgtaa ctcaaaggaa
    24181 gtcccttgac gattgcgaag gatgcagtgg cagagccaag gaccgatgcg gccttggccc
    24241 gcatcacctc tgccgtatcg gggttctttt tctgcggcat gatggagctc gtggagcaga
    24301 aggcgtcatc gagctccacg aacctggcaa agcctgagct ccacaccacc agctcctcgc
    24361 agacccggct tgcatgggtc atcatgatag agaggcagga gagtacctcc agggcaaagt
    24421 cgcgcgagga aacggcatcc atggtattct cgagcactcc ctggaaaccg aggagcccgg
    24481 cgacaaattc tctctggacc gggtaactcg tggaggcaaa agcggcggaa ccgagcgggc
    24541 accagtcaac ccgtgagagc gccccggaaa gcctctggta atcccttgca aacgctgcct
    24601 cgtgggcgag aaggtggtgc gcaagcgtgg taggctgggc gtgctgaagg tgggtgaacc
    24661 caggcatgac agactctatg tgctccccgg caaggtcaag cagcacctcc ctcatacgga
    24721 tgaggatgga gagctggcgt acgaggtcat ccctcacgcg aagccggatg caggtcgcca
    24781 cctcgtcgtt cctcgatcgc cctatgtgca gccttccccc tgcatcctcg ccgatagtgg
    24841 cgatgaggag ggtctcgatg cccgcatgga tatcctcgaa ccggtcgtcg aaggccgctt
    24901 cagggatccc attttcatac agctccagga gtgtttccag tagtcttccc gcctgctcct
    24961 ttccaataat cttctgccgg gagagcatca gcacatgggc gatgtctacc aggatgtctg
    25021 catcggcgat gcaacgatcc gcacccattg aagagagaaa atgcatcatc tctcctgaac
    25081 gctcaccggt gagccgtccc ctgcgcacca cgtcctgctc catcctaaac cataacctcg
    25141 cgtataacta ggacgctgga atgagattaa agccacgctt tccgggccgc aagttcaatt
    25201 gccctctgga ctgattcacg agggatgatc gtggcgaccg gtaccttcac cagctgctcg
    25261 atgacgctgc tgacgatggg tgcgcagaca atggctaccg ccccctcccg ttcggccctg
    25321 accgcggcga cgatggcctc ttccattgtg tggaccggat actcgcggat tctaaccttc
    25381 ctgccatcga cttcggcatt ccgctccagt atcacgtcga gaacaggccg tgccgcgatc
    25441 aggccgataa actccccctc ggaagagagg gtgatcctcc tcattgcctg gacgatcgca
    25501 cgcagcgtgg tgagattcgg agacctcttt ccatgcagca tcttgtagag ggagctcgga
    25561 gcgaccccgc tcttttcggc aagctccctg acgcttatcc tcaggtcacg ctgcatgacc
    25621 tcgcggaggg catgcacgaa ctcatcgtca gagatgagtg ctgcattcac gagcctgtcg
    25681 accgggtctc tttgcatggg agttcctgga atgtgagttt gggagttccc gtggtataac
    25741 ctttttgtcc atttgcaccg gatatgatct ctccttgtca caatatttaa cccaataata
    25801 ctattaatct caaatttttg ctacaaatag acagtaaata gtcatctttc ggagaattta
    25861 tttgtgcata tgagaacagt gtgtgcatga agtattcgca tgtgtgtgca atcatcggtg
    25921 tcatactctg tgcggccctg atcgcaggct gcatcacacc tacccagcac caggggactc
    25981 ccacccccac cgtttcccct tcaggtggcg atgtaatcgt cacctacacc aaggggaccg
    26041 ggcctatgcc cactctgctt gggacggacc agatcgacgg ctatatcgcc tggcagccct
    26101 ttgtcgaggt cccgcccatg gcagggatcg gaaagatcct tctgtattcc ggcgacctgc
    26161 ctccccaggg caagtggaag gaccacccat gctgtgtatt caccgccaac cagaaggtga
    26221 tcgcccaaaa tcctgacctc accaatgccc tgactgcatc gatgatcctc gccacccagt
    26281 accttgaaga gcaccccgag gagagtgccg agatcgtggc ggactggctt gcagggaaag
    26341 gcaactttac ctacggagag atctcggtct catcggtaga cgtgctgaag cgcgccttcc
    26401 ccacggtgaa gttcgtgaat gacccgaccg acaagtggat ggactcaaat atcgaattcg
    26461 tccatgcatt gagagagctg gaggttctca ccggttccct gaaggcctcc tctgacgctg
    26521 attcaaagac cattctcttt gacacggcgc catacaatgc ggcacagtcc atgatcggcg
    26581 ccgggaagat taccacaccc acacccgtga agggatcggt tggaatcggt tacctgatgt
    26641 ctgaccacca cgcggccctg ttcgtggcca tcaagaagtg ggagtacttc aacgatacat
    26701 acggcatcgc catcaaaccc agggataccg ccgcatccag gccggatatc gccgacttca
    26761 tagtcaacgg agaaaaagtt gctgagatca agctgatatc gggtgatgca ggcccgcagc
    26821 tgatgcagtt gatggcaact gataatatcc agtatgcact ggtcggaaac ccgcctgcaa
    26881 tctccgcggc cgacaagggc acccctgtca agatcatcat gtccatcaat tcagagggct
    26941 ccggagtcgt ggtgaccgag gactctcctg ccaatgactg gaacagcttc gttgcatggg
    27001 caaaggagcg ctctgcggcc ggaaagccgc tcaggatcgc ggctcccgga aagggatcaa
    27061 tccaggacgt catgatccgg tactccctcg aaacaagtgg tttatcaata aaggaaggat
    27121 aaccttaggg gacaatgagg tatctccggt tcctcctccc cattctcctt gtcattgggt
    27181 gggaggttgc gtcgtgggtt gtcaacaatc cctttatcgt cccgtccctt gagaccgtaa
    27241 tccccatcct cctgcacccg ttcagccccg aatatacgct tgggaccggg agcatggtgg
    27301 agaatgcact tatcagtatc cagcgcgtgg gccttggttt ccttgtcgcc gcagtcgtgg
    27361 cgataccgat cggcatcctg atgggaaggt cggcattcct caacgacttc ttcgattcaa
    27421 caatcgagct gctcaggcca attcccccac ttgcctgggt ccctctcgca ctcgcctggt
    27481 tcaagatcgg gctcacttcc attgtcttca tcatcttcat aggtgcattc ttcccgatac
    27541 tcctgaacac tgtcgatggc gtgaaatcgg tgaagaagac atggctcgag gtcgcgacca
    27601 cgcttggggc gaaggaacag cagatcatga caaaggtcgt gctccccggc gccgccccca
    27661 ccatctggac cggccttcgc gtggggttcg ggatcgcctg gatgtgtgtc gtcgccgcgg
    27721 agtggctgcc gggcatctcg caggggctcg ggtatcttat cctctatgcc tacaactttg
    27781 gccagaccaa cctgatcatt gccgggatgg tcgtgatcgg cgtaatcggc ctcctgatcg
    27841 actttgtctt caagcacgtc gagaagagat ggttctcgtg gagggggctc gagcgatgag
    27901 ccgggtacgg aaaacattgc cgggtggtga atgcctgtga ccctggtgct caaggatctc
    27961 tcaaaatcct tcgcagggga agggaaggca gaggtggaag cgctctccca catcaacctt
    28021 gaggtgtcgg acggcgagtt cgtctcgatc ctcggccctt ccggatgcgg gaagactacc
    28081 cttctccgca tcatcgccgg gcttgaccat gcgaccggcg ggacgatcct tctcgatggg
    28141 aataagatcg aaggcccctg cccccggatg gccatgatat tccaggaata ctctctctat
    28201 ccgtggcgga cggtaatcga caacattgcg ttcggcctcg agatgaaggg aatggagaag
    28261 cagaagcgca gggagatggc ttttcagttc ctggaacttg tcggtctcgt cgattttgcc
    28321 ggaagttacc cgtacgagct ctccggcgga atgcgtcagc gggtcgcagt agcccgggcc
    28381 cttgccatcg aaccacagat cctcctgatg gacgagccgt tcggggcact ggacgcccag
    28441 acaaggaaca tgctccagaa ggaacttctt gagatctggc agaaaaccag gaagaccgtc
    28501 atctttgtca cccacagcgt ggacgaagct gtatatctct ctgatcgcgt ggtggtgctc
    28561 accgcccgcc ccgggaccat aaaagacatg gtccatatcg atctcccgcg cccaagggag
    28621 aggacaggcg cagaatttgc aaggatccga cgttatgtcc tcgatcacgt ggagaaaact
    28681 ccgttttcca gtaaggttta agaagatata tccccatttt ataaagcaca gacagggata
    28741 aggagtattc agtcatggta cgaaaaccag gaaagatgta ccggaacatc tcgaaaaaag
    28801 cgtatacccg ccgggagtac atgggcgggg ttcccggcag caaagtagtt cagttcgaca
    28861 tggggaatct gtccggggag ttcccgatgg aggtcagcct cgcggtagag gaggcctgcc
    28921 agattcgtca caccgcgctc gaggcagcac gtatcaccat caaccggcac cttatcaagg
    28981 accttgggag gatgaactac cggttgaagc tccgtgtgta cccgcatcac gtacttcgcg
    29041 agaacaagca ggcaaccgga gcaggggcag accgtgtatc cgaggggatg cggctctcgt
    29101 ttgggaaggc agtcgggact gccgcacgag tcgcacctgg acaggagata ttcaccgtct
    29161 ataccattcc ccagtacctt ccccaggtga agaccgcact caagtcgggc ggccacaaac
    29221 ttccctcccc ctcgtacatc aacatatccg agaggactgt tcccgagtcc cctgcacccc
    29281 agcaggcata aacccctttt ttggctattt accccaggtt ttttcccctt tatctatgcc
    29341 ggttctggct gtgcaatcaa aaagatctcc aatccaggtg cctgccaggt aatgaaatac
    29401 gagggggaac ctgccgaagg gaatgtcccc tgttcgggcc ttatcacaat ttatattccc
    29461 attcactcca aaccttttat cagcatgatc ccatcccctg gccccgccct cttcgctgcc
    29521 gtcacaaaag caacctgcga ggcattccgg cgggcagaga ttacccttcc ctcggacgtg
    29581 atagaggcac tccgcaatgc aagggagagc gagacaagcg agatcgcaag gggggaactc
    29641 gcgaacatcc ttgccaacct ggagatggca gagaggctct ctgtccccat ctgccaggac
    29701 acaggggttc cggtcgtcta tctcaccatc cccccccacg tcccgctcac aaccgagata
    29761 tacggggcga tcgcagaagg ggtgaggatg gcgactgaag aggtaccgct ccggccgaac
    29821 gtggtcgatc ccctcacgcg ggaaaattcc ggggataata ccgggacagg aatgcctgcc
    29881 attcacgtca ggcccggcga tagcctcacg atcacggtgc tgcccaaggg cgccggtgcc
    29941 gagaacatgt caaggatctc catgatgctc ccatcagagc gggaacagat cccgaagttc
    30001 ttggcagaga caatgctgat cgccggtggg aagccctgcc ccccggtcat ccttggcgtg
    30061 gggatcgggg gaacgttcga tgaggctgcg tcgctcgcca aggaggcact gctcgatccc
    30121 atagacacga tgactcccta tgagcaggaa ctacttgatg cggtcaacat gctgggcata
    30181 ggcccgatgg gcctcggggg gagtaccacg gcgcttgcgg tcaaggtgaa gaccgcgggg
    30241 tgccacacgg cgtctcttcc cgttgcggtc aatgtccagt gctgggcgtg ccgcagggca
    30301 accgtggagg tgcccacttg aacgtcatgg acctctgcac cccgctctcc catgaggtgc
    30361 tgatgctccg tgccggagac cgggtcacgc tctcggggac ggtgtatacc gcgagggacg
    30421 aggcgcacct cagaatgatg gaagagggca tcccctttga tccccacggt gcagtggtct
    30481 atcactgtgg ccctgttatc cagggacagc gggtgatcgc cgcggggccc accacatctg
    30541 ccaggatgaa ctcgctctcg ggattcctga ttgagaaggg tgtccgcgca ctggtgggaa
    30601 aggggggaat gggccggcag gtggtagacc agctgaaggg ccggggggtg tacctcgcgt
    30661 ttaccggagg ctgcgccgca cttgcagcct cccggatgac cctgaaagga gtcttcttcg
    30721 aggacctcgg catggcagag gccgtgtggg ccatcgagct cgatcgcctt ccgctggtcg
    30781 tggggatcga cgcggccgga aacgatcttt tcgaaggggt tcaccggaat gcgcaggccg
    30841 ggttcaaaaa gaggttccag gcaaggaggg atgtgccggc atgagactcg taatcgatga
    30901 acgaaggtgc aaggggtgca acctctgtac actggtctgc ccgtaccgga tatttacccc
    30961 tggaacgcag ccgaatgcca gggggatcgt tgttccggtg ctggaccggc cggaacgctg
    31021 tgcgaactgc cgtttgcagg cgctctatgg ccgtactctc tgcggaatgt gccagatgat
    31081 ctgccctgac caggcgatct cctgggtcga ggagaagcct ggcgaggccc ggaaggtggc
    31141 gattgaattt tgaccagaat tgacttcatg cagggcaaca cggcatgtgc ggagggggcg
    31201 ctcgccgccg ggtgccgttt ctttggcggg tatccgatca ccccatcgac cgaggttgcc
    31261 gagcacatgg cggccaaact cccgaaggtt ggcggcgtgt tcatccagat ggaggacgag
    31321 attgccggga tcgcctcggt aatcggggca tcatggaccg gtgcaagggc gatgaccgcg
    31381 acgagcggcc ccggattctc gctcatgatg gagaatatcg gctttgcggt gatgacagag
    31441 accccctgcg tcctcgtcaa catccagcgg ggcgggccgt ctacagggca acccaccatg
    31501 tcagcgcagg gggacatgtt ccagtgccgt ttcgggtccc acggggatta cagcatcatc
    31561 gccctctcgc caggaagcgt ccaggagatg tacgagctga cggtgaaagc cttcaacctc
    31621 gcggaccgtt tcagggtccc gacattcctc atggccgacg agatcatcgg gcacatgagg
    31681 gagcggatcg agatcccgga cagcgtaccg cgggtagaac gaaaaccgct cgtaaaaggg
    31741 gtgctcccat tccatgcgga tgacgacatg gtacccgggt ttccgcagtt cgggacggga
    31801 caccatgtcc acgtgacagg cctcacccac gatgagcggg gatatccctg tgccaccagc
    31861 ccgagcctcc acgaggacct cgtgaagagg cttgttgcaa aggtcgaatc cgcccgccac
    31921 gagattgccg attacaaggt cactaatcct gatgcagagg tggtcttcat cacctacggg
    31981 tcgccctccc ggacggttga ccaggtgctc catgaccgcc aggcggagag gattggccat
    32041 ctccggttcc gcatggtctg gccgttccca gagaacgcgc ttgagcagtt cccgaacgcg
    32101 aaggtcttcc ttgttcccga gcagaacctc gggctgatgg caagggagat cgagcggcat
    32161 acctgtgtcc cggtggtccc tctcccaagg ttcggggggg ccctccacac tcccgctgaa
    32221 ctggagaagg ccatggaggc gtggcgatga catatgatga ctggctccgg gcagataggc
    32281 tcccacatat ttactgcgcg ggatgcggga acgggactgt gataaactgc actttgaacg
    32341 cagtggaccg gatgggctgg aagaaggagg agaccatatt tgtctcaggg attggctgct
    32401 cctcccgtgc cccgggatac atcatgacag attccctcca caccacccat gggagggcgc
    32461 ttgcttttgc caccgggata aagatggcaa accctgacct gcacgtcgtc gtctttacag
    32521 gggacgggga cctcgctgcc atcggaggca accacttcat ccacgcctgc cggaggaaca
    32581 tcgacctcac cgtggtctgc atgaacaacc agatttacgg gatgacgggg gggcagggga
    32641 gcccgaccac cccgagagga gcgctctcca ccaccacgcc gtacggctcg caggaaccgg
    32701 cgttcgacct ttgcgaactc gcatcagcag ccggtgcgaa ttttgtctcg cgctggacct
    32761 cctaccacgt caaggagctg acccggtcga tccatgaagg gcttggcata cccggcttct
    32821 cgttcatcga gacgctggtc cagtgcccga ccaactatgg gaggagaaac aagttccggc
    32881 aggtgatgga ccaggtcgag tatttcaagg ctcatggaat cctggtgcag aaagccgcaa
    32941 ggctccgaga agaggggaaa gcaattccgg gagatgcgat cattctcggc gacatcgtcc
    33001 ggagggacag gcctgcaatg ggggtgaggt gatgaggcac gaggtgcgct tctcaggatt
    33061 cggcggccag ggcatcatcc tctctgcggt gatcctcggc agggctgcgg tgatgtacga
    33121 caggaattac gcggtccaga cccaggtcta cggtccggaa gcccgcggcg gcgcttccat
    33181 gagcgcggtg atcatcgatg accaaccgat tctctacccc aaggtaaccg atcccgacat
    33241 cttcgtgatc atgtcccagg agggttacga gaagtacggg gcacgggcga caaaggaggc
    33301 ggtgatggtc cttgactcat ccctcgtcca gtccaggcct gcgtgcaggt tcatcgagat
    33361 ccctgcaaca agggaagcaa agcagaccct gggccgtgat attgtcgcca atatcatcat
    33421 gctcggggcg ctggtgaaga caacgcacgt ggtaggagag gaggccatcg agaaggctat
    33481 cctcgattca gtgccgaaag ggaccgaggc cctcaacctg aaagcaatga aacttggatt
    33541 tgaactcgcg gagaagggga agatgaaatg aaactgttgg aacacgaggc aaggaacata
    33601 ttcagggagt acgggatacc tgtcccggca ggctttctca tccgctcccc gggagaactc
    33661 gcatcccgca tgggagaact gggtgaacga ttcgtgctca aggcccaggt ggaagtcggg
    33721 ggacgcggaa aagcgggcgg gatcgtcgtc gccgatccca ccacagggga gcgcatcgcg
    33781 ggggatctct tcaggaaaga gataaagggc gtccctgttc gcgaggttct tgcagtggaa
    33841 taccttgata tccagaagga atactacgca agcatcgcca tcgaccgctc cacgaagcag
    33901 gccctcatcc tcttcactga agaggggggg gtggacatcg aggcactcgc aaaggagagg
    33961 ccggaggcag tgcgaagggc ttcgcttccg ctcctgatgc gcgacatccc gcctttcatg
    34021 atccgtgacc tgctcggggc tgctccaaaa gagatcggaa aggtgttaaa ccagctctac
    34081 agggtattca gcgagaagga tgcccttctt gcggagatca accccctggt cacgacctca
    34141 gagggcatct ttgccgccga tgcgaaactc atcgttgatg ataatgcact ttcccgccag
    34201 ggcatcaaac tgaaccgtga cctgaccgat cgggaacgcg aggccgagat gcacgggttc
    34261 tcgtacgttg agctcaaggg cgatatcggg gtgataggca acggcgccgg cctcactatg
    34321 gcaaccctgg acctgatcga gtattatgac gggaaggcag cgaacttcct cgacgtaggc
    34381 ggcggtgccg accaggagcg ggtaatgcat gccgtaaggc tcgttgccgc agacccatcc
    34441 gtgaacgtaa tcgtcgtcaa cctcctcggg ggcatcacga ggtgcgacga agtagcgcgg
    34501 ggaatcatca gtgcagggat cagccagccg gtgatcgtca ggctcgccgg gacaaacgag
    34561 gctgagggac gacagatcct cgatggcagc gggtaccgca tgatggagag catggagcag
    34621 gcagtgaaag cggcagtgga ggcggtccag tgatctacgg agacaaacac accggcgtga
    34681 tcgtcaccgg cgcgacagga aagcagggtg ccttccacat tgcgctgatg aatgagtatg
    34741 cccggcaggt aggaggagag ggcgtggtcg ccggcgtgac gccagggaaa gcagggcagg
    34801 aggtcgcggg cgtcccggta tacaacagta tacacgatgc actaaaagag cacgacgcga
    34861 gtgcaagcgt cctctttgtc ccgggctttg ccgcgggcga ttcgataatg gaggcagcaa
    34921 gcgccggact ctcgcttgtc gtggcaatca ccgagcacat cccggtacat gacaccatgc
    34981 gggcgatctc gtttgcccgt atccacggct gcgacgtgat cggcccgaac tgcccagggc
    35041 tcctctctcc aggggagata aagatgggga tcatgcctgt ccacctctcc tcgcggggac
    35101 acgtaggggt catctcaagg agcggcacgc tcacctacga gattgtcgac cagctgactc
    35161 gggcggggct tgggcagagc accattgtcg gcatcggagg tgacccagtc atcggccaga
    35221 ctttccgcga cgtgctccgc cgcttcgagg aggatgacca gacagaggcg gtggtgatcc
    35281 ttggggaggt cgggggttcc cttgaagaag agggtgcgaa ggcgactgat ctgcccatcg
    35341 cggcatatat cgcaggaatc tctgccccac ctgagaagcg gatgggtcat gccggggcca
    35401 tcatagaagg aggcgagggg gacgcccgct cgaagattga gcggctcagg agaatgggag
    35461 tccccgtggc atcgaggcca tccgaagtcc cgcgcctgat aaaggagata ctctgatgtc
    35521 tcaccgatcc cggctcccgg accctgaaaa ccatttaacc catcacgtag taccacatgt
    35581 ataattctct accggaggaa cgatgagcga tcacccaagt accggctacg atcccatgac
    35641 aggagaagag gagctccagg gccccgagcg atacttaagg cccggcgaag agctcctgat
    35701 ttacacccct gatatccaga ttaagaagtt caggttcgat gcctatctga cgaacacaag
    35761 gctctttctc accgaccagg atgaaaagac ccctggtgta actgcaaagg agataccaat
    35821 cgagtccgtg gcagatacct accttgagca ttcccctgtc caggagccga tccttgtcct
    35881 ctccatacgg acttcggacg acgatctccg tacaatgaag atgacatttg tccataacgg
    35941 tgccgacagg ctcccgcagg cagaggagtg gatgcacctt atcatgcatg ggcatgcaaa
    36001 gcgtccttcg ggtggcaccg cggtccggcc cgcgtcgccc cagcccgttc cagccatatc
    36061 cccggaggcc cgtgcactct ctgagacgat catatatcct gaaaagacca ctgttgagcc
    36121 aggccccatc tcatcagcgg gtcccgctgt tgtccaagta gagggacgaa cacgaacacc
    36181 cccgcctcag cccaggcaac ctgaagcgcc acgggcggca tcaacacctc ccggggccgc
    36241 gacaacccag atacaattct gcttccactg tggtcacaaa ctgcctccga atgcaaattt
    36301 ctgcccatat tgcgggaccc gccttcacca ggccggtgca ccccttcaat cgcctccgcc
    36361 aatccaaatc caaaggaccg aagatgagcc tgccagcctt gcgtcaaagg agaaagagaa
    36421 gagaggtttg tttggctggc ttttccgaaa gaaatagatc ccctcctttt tcgtcttccc
    36481 gacaactcta aggcttgttg ccggaaaacg cgtaattcat tgaaggatta aaaaaaggat
    36541 aatacctgac ttgtggcaac ctgccaactg ttcttccagc cggagctata ttcgcgcctt
    36601 cctccgttca tgagaaaagc ccaggcatca aaaggaatga ttatgttacc ttccgtcgtt
    36661 ttaataatga ttacgcgtcc ggtgatcgag ggatttcatt gagacggaat ataaagatcg
    36721 aagcattgca ccagatcccg ctggaacgcc aggagatcga actcgttgag cggaaatgca
    36781 tagggcaccc tgacagcctt gccgatggga ttgccgagtc catcagccgg gctctctcaa
    36841 aggcatacct ggaggagtgc ggtgcaatcc tgcatcacaa taccgaccag ggcgagattg
    36901 tggccggaga gtcccagcca aaatacgggg gagggaaagt catccggccc atctacatgt
    36961 taattgacgg aagggccacc aagcagttcg atgggatcaa tatacccacc gatgcaatcg
    37021 ccgtcgaggc agcccggact tacctccgcg agactctcac caacctcaac atggagcggg
    37081 atatcatcgt cgactgtcgc ctggggacag gttcaacaga cctcagggat gtcttcaagc
    37141 cctgccagaa cactccgccg cgcgccaatg acacgtcttt tggtgtaggg catgcgccat
    37201 tctccgagac cgagacgatc gtcaaggcag tgtcagatta cattgacggg acgttccgcc
    37261 cccggtaccc tgcagtcggg caggatatca agatcatggg gcttcgggac ggggacacca
    37321 tcacgctcac actggggtgt gcaatggttg accgctattg ttcaggcctc cccgagtacc
    37381 tcgaatacaa ggatctcctc agagagcaca tcctcaatgt cgcacgccag cacaccggac
    37441 gaaaagtcat cgccgctatc aacactgccg acgacatcga taccgggagc gtctttttga
    37501 ccgtcacggg gacatcggcc gaaatggggg acgacggctc ggtggggagg ggaaaccggt
    37561 gcaacgggct cattaccccc aaccggccca tgagcatgga ggccaccagc ggaaagaacc
    37621 ccatcaacca tatcggaaag atttacaacg tcatctccac gcagcttgcc caggagtgcg
    37681 ttgcaaaggt tgacgggatt gaagagatct atatcaggct tctctcccag atcggcaagc
    37741 caatcgatca acccctcgtt gcaagtgtgc agatcctgcc aggcgagggt gtggatatga
    37801 agaccatcag cccggatatc gaagccatca tcgacaatgg gctggccgag atcacctgca
    37861 tcaccgagaa gatcatccgt ggcgagatca agacgttctg aaggtgagga gagccccggg
    37921 ccggttgcca ggccccaggt tctgcgcctg gcaatcccga acaagggccg gatatctgtc
    37981 ccagtcaacg agctgatgga gaagagcgga ctccacctcc tcgatgtcgg ggaacgccgc
    38041 ctcgtttcac gcactcccga cccccacgtg gagatccttt ttgcgaggcc cgtggatatc
    38101 cccgagtacg tggccaacgg ggcagctgac ctcgggatta ccgggcacga catggtcctc
    38161 gagcggaaat ccgatgtatc cgagctcctt gacctgaaaa tcgggtccgc gtccctggtg
    38221 cttgccgtcc gtgaagatgc cgggtatacg aatccgttgg atctcgaggg aacgagggtt
    38281 gcgaccgagt ttcctgtcat ctcaaaggaa tatttccagg agaagggtat ctcagtcacc
    38341 ctggtgcctg tcggcggggc atgcgaagcc acaccgtatc ttgggatagc cgaggcgatc
    38401 gtcgacctct ccagctcggg gaacacgctc cgttcgaacc gcctccgggt aattgatacc
    38461 gttcttccca cgaccaccat gctgatcgca aacccccagt cccttgcctc gaagggagga
    38521 aagatcgagg agattgtcct tgctctcgag agcgtggtcc gggccagagg gcagtgctat
    38581 ctgatgatga atgcgaagcg tgcctgcgtc gatgaggtca catccgtcct cccggggctc
    38641 tccgggccga cggtgatgga ggtggcctcg cggcaggaca tggtcgctat ccatgcagtc
    38701 gtcgacgaag agcgggtcta ccagttgatc cacctcctga agcgggcagg agcgcgggat
    38761 atcctggtca tgcccatcga gcggatgatc ccctgacatg cgcatatttc ccgccgtcga
    38821 tattctgaat ggccagtgtg tccagctggt gcagggaagg aaggaatcgg ccacagtctt
    38881 cggtgacccg ctctcctgtg cgcatcgctg gctcgacctc ggcgccaggt gcctgcacgt
    38941 cgtgaacctt gacggcgcat ttggaaaatc ctcggcaaat gcaggaaaga tcagggaact
    39001 cgtcagagag acgggcgtcg agatacagct tggcggggga atacggtctt tctcggatgc
    39061 ctcctcgtgg ctcgagaccg gcatcgagcg ggtcatcctc gggaccctgg cgacccggga
    39121 tccgcctgtc ctgaaacggc tgagcgatga gttcggcagt gcccgcgtca tggcgggtgt
    39181 cgacgcccgt ggtggggaga tcaccataga ggggtggcag aaagccgggg gagactatct
    39241 tgtatgggca gaacgatttg agaatatgag tgtgggatca ctcctcttta ccaacgtgga
    39301 cgtggagggg ctgcagtcag ggatcgatcc cggacccgtt gcccggctgc tgcggcgcac
    39361 ccgcctccct gtcacggttg cgggaggtgt caccacggcc ggggacatca gggtcctcag
    39421 gtcccttggt gcttccggcg tagtgctcgg gtctgcactt tacagcggga ggcttcacct
    39481 gcatgaagcc atggaggcgg cagaatgaga tgcgtaacct tgacaagaaa gacaaaagag
    39541 acttcaattg atctggtcct cgaccttgac agcccgggga gaggagagat ctccactgga
    39601 atcgcgtttt ttgatcacat gctcaccgcc atggccactc acggacgttt cggcctctcc
    39661 tgcagggcga aaggcgacct cgaagtggac gtccatcaca ccatagagga caccgggatt
    39721 gtccttggcc aggcattcag ggatgcgata ggggacggca gggggatcac gaggttttcg
    39781 catgcgatca tccccatgga tgaatcgctt gccactgttg ccctcgattg cggcggtcgg
    39841 ggatacctcg tctacagggg tgcattcgct catccccggg tggggggaat tgagcgagaa
    39901 atattcgagc acttcttcta cagcctctgc atccatggcg ggatcaccgc tcaccttacc
    39961 atggagggga agaacgatca ccacaaatgc gaggcactct tcaaagcatt cggcattgcg
    40021 atcggtgcag ccacccgcct tggcggagat gccgatcatg taccgagcac caagggagtc
    40081 ctctgatttt aaaaaatggt gcaggagagc ggaaaagggc atttcagggt ccctcccccc
    40141 atgggatccc agaaataacc cgctcaattc cttaaaaaaa gcgaagggaa ttgttttcaa
    40201 caggtgatga aagcctggta agcgcttctt ttgcagttat gcgtacgaaa cagttctcgt
    40261 caatacatgc acgggcgagc ggctcgattg cccggggatc tccgatcatc cccagtgccg
    40321 atgcagagat gaaccgaata tccctgtcgc ggtcctggag catggatatg aggggttcga
    40381 cactgcgcga gtccccgatc ttcccaagag catgtgcggc tgctatcctg acccgtttat
    40441 ccccatcatt caacgcaagg agaagaaagt ccaccgcggg ttgtcctttc ttccccagtt
    40501 ccgtgattga ctggactatg tccggactgg tcttctctgc cacacccagc agatgccctg
    40561 tcatcattct ccccctacaa caaccctctt tcgaaggcag atgtaacata aatcgaaaga
    40621 gtaaaaatga atgcgccaat cagctatgca cagcatagtt ttatcaattc gttcgtattc
    40681 ccataggttc ttttcacggg acgcgtccct acctggggaa tcgatcagaa aagggtttat
    40741 gccaccttga ttgttgggtt gctggtcagt gtggtgaggc aatttcaggg aaaaggtatg
    40801 ggttatgcgg acttggccgc aagttccacg tcctcttcct tgatcgtctt tctccctgcg
    40861 tgaagtgcaa gcctgttggc ttccttggtc aggttcgcaa tatactcttc tgcctttgca
    40921 acgagtgcag cagcagcatc gctgccgact ctttcagcac cattcttctt ggcaattctc
    40981 acaactgcag caataggtaa atctgccatt gtat
//
LOCUS       DAOQYR010000012        39060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_1200_length_39060_cov_9.474837, whole genome shotgun sequence.
ACCESSION   DAOQYR010000012 DAOQYR010000000
VERSION     DAOQYR010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 39060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39060
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1200_length_39060_cov_9.474837"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..1374)
                     /locus_tag="PKM87_02705"
     CDS             complement(166..1374)
                     /locus_tag="PKM87_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29007.1"
                     /translation="MAIGKGAYLKAITSSRLGSSRILDRTMEGSTPPSVFIGSWNYPK
                     VYAGPLIAPVREECGIMDTPEAWIPAGWSQEEILSCRLDLVRGKRVCDVTRVDDPDAC
                     RLQEIALSAGSIESQALFSETPAGTSFSEEHTPFGPSAHLLEFEAESSRFEPHLERCY
                     YDSDLPASEAILTLHRDGVPFSSIQKALSVGSLGTGGKRRMVPTRWSITACDTTLADR
                     FLSRVRHNRLIDTIRVHEFSSLNNHYAVILLPTPWQYEWMEAFLHIRGNEELLFSDYE
                     EFRGKKGYSCVGGCYYSCKMAVLEGLEKEGVQAGAIVLREARKGYIPMGVFNVRENVR
                     QAMHQPFREFEDLPSALADVSMRMTLPMGRFVNESTVLKSQFRARQTTLDSFFRPGKS
                     SVCIPPDSTA"
     gene            complement(1438..1989)
                     /locus_tag="PKM87_02710"
     CDS             complement(1438..1989)
                     /locus_tag="PKM87_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29008.1"
                     /translation="MHEPSRKIVHMFFGVGIAALAYLLPKDLIVLLLAASIFIGFILA
                     EGVLRGYRIPLVSLLIRNLERDGEYPGKGAFYFAASALFCVLFFDAEISVPAILSLAV
                     LDGAATLVGLRWGRHRITNGKSVEGSAGGVLVNILVLVFFLPPLPAIAASVAAGAVEL
                     VSPVDDNLTIPVCICILLTLMGV"
     gene            2060..3301
                     /locus_tag="PKM87_02715"
     CDS             2060..3301
                     /locus_tag="PKM87_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNQ29009.1"
                     /translation="MSKRGSILHAMTGAGDLRFRGAGDVITESLSVYGIQVIRSTGEF
                     WTARQRQGSSLHEVSYRGCFKPQLPAYFINLLTKPGDLVYDPFSGRGTTVIEAGLLGR
                     AVAANDANPLSRLLTRPRFFLPDTADVKSRLEAIPIDTGAGAEMDLSMFFHPGTEAEI
                     SSLRNYLLERERDGDIDHVDDWIRMVATNRLTGHSSGFFSVYTLPPNQAVSPMRQIRI
                     NQKRDQVPPYRDTRALILKKTLSLLKDVSEIQKERLRKSGENALFLSGDSRRTPGIGD
                     GTVQLTITSPPFLDVVQYSSDNWLRCWFNGIDPGTVEKNLTVTRSLPDWRKVMQQTLY
                     ELFRVTRSGGHIAFEVGEVKGGTVRLDETVVPLGVRAGFSCEGILVNLQDFTKTSHIW
                     GIGNNAHGTNTNRIVVFKKEG"
     gene            complement(3308..4462)
                     /locus_tag="PKM87_02720"
     CDS             complement(3308..4462)
                     /locus_tag="PKM87_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ29010.1"
                     /translation="MPRVTVYYTQNCPYCRMLKAFLEKHSVDYMAIDVGTDREQAGKM
                     VELSGQYGVPVTTVDDEVIVGFDAQRLNELFGTAREGDIYDVLIVGAGPAGLTAGVYC
                     SRKMVKTVIISENIGGQALESWAIENYMGYRMVTGEDLMHKFEEQVRGEHIHLELDRI
                     QAIRREGDLFVVDTATGGTYSTKTVILAQGKRPRKLGVEGEERYLGRGLSVCSTCDGP
                     LFRDKVVAVVGGGNSALQTAIEMSKIAREVHLVVRSTIKADEAYVKQYEQQGNIRTYL
                     HHTVAALHGNAMLNGITLKDRESGKETTISLDGVFAEVGWIPNTDFLEGFLRLNGQKE
                     IEIDINCHTSVPGVFAAGDVTNIRTKQIITAAGEGAKAALEAYDYLVKLE"
     gene            complement(4626..5102)
                     /locus_tag="PKM87_02725"
     CDS             complement(4626..5102)
                     /locus_tag="PKM87_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29011.1"
                     /translation="MMILRISLLILACVLVLAAGCLGNAPGSPITTASPAPTTMPPRT
                     PTLSLVPEPTDIMPPTYSVTVQVVKNTIATNPTITVTYEGGQGLAFTQSMTAEVIRSD
                     GTVDQQTVQSPQMGSEIVLTGTTRTDRVIVYVTMVNGVTYRVFDKDMPFQPINPQY"
     gene            complement(5215..5715)
                     /locus_tag="PKM87_02730"
     CDS             complement(5215..5715)
                     /locus_tag="PKM87_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNQ29012.1"
                     /translation="MATMENAMEAFAGESQANRKYAAFADQAESEGYKNIARLFRAAS
                     EAEAIHAKKLLKAMGKIHATKENLAESVSGETHEFKEMYPAFLKEAESEKKSDIGLVF
                     TYAMKAEEVHATLYKEALNNLNHGQDMSNRTVYICPVCGNVFLGTAPEKCPICMAVRK
                     VFRTVE"
     gene            5867..6586
                     /gene="radC"
                     /locus_tag="PKM87_02735"
     CDS             5867..6586
                     /gene="radC"
                     /locus_tag="PKM87_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNQ29013.1"
                     /translation="MRAIFLVPLHMLPLRSQMKRMRELDRCDRPREKIAKKGARSLSD
                     QELIAAIIGNGMQGRDVFQVAREIARLFRESADTITYNDLTSIRGVGEGKASQILASM
                     ELARRYLKNEEEARVKIQAPADILPLVAGLNGQRQEHFICVTLNGAHEVIATRTITVG
                     LLNHSLVHPREVFADAITDRAAAVICVHNHPSGTLEPSSQDLAITRQLRDAGDLLGIR
                     LLDHLIVARTGYLSLKEQGHL"
     gene            complement(6587..7189)
                     /locus_tag="PKM87_02740"
     CDS             complement(6587..7189)
                     /locus_tag="PKM87_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNQ29014.1"
                     /translation="MGYRIIGLLGSPLPHGNTAKLLDAALKGASDAGCGVEKIEVAHL
                     AFRPCREIFYCREHETCAMKDDMTAIYEKFRELDSLIVATPVMTMGIPGALKSFMDRF
                     QVFFMAKYMRKKALVPKERRAHRRGLYLGISGMKVANVFDGAKMTMSAFFSIIDVQYW
                     DDLLVPDMDTIQDITTRPDLLESAYKKGYEMGRLLVEGSA"
     gene            7344..7577
                     /locus_tag="PKM87_02745"
     CDS             7344..7577
                     /locus_tag="PKM87_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29015.1"
                     /translation="MIDLTEKAKAGQKVGPGKYVCLDCGRELTLDAVEQDLRKCPSCS
                     CEEYQCFPMTHIRPDIKTPEDVMNPPKRGKSNQ"
     gene            7688..7813
                     /locus_tag="PKM87_02750"
     CDS             7688..7813
                     /locus_tag="PKM87_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin FeS4 iron-binding
                     domain-containing protein"
                     /protein_id="HNQ29016.1"
                     /translation="MVNVSKEGQQFECEICGNVVVVKVVGGGELVCCGQPMKLKK"
     gene            7821..8174
                     /locus_tag="PKM87_02755"
     CDS             7821..8174
                     /locus_tag="PKM87_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNQ29017.1"
                     /translation="MAKETKNIKDLEKMIGKVPKFFKELAEKDPMMYEMVMKLEGHIW
                     DDGALTRKTKKLIAIAIAASLRDQHATRAQLAGAANLGIKKNEVEEALRVAFLLAGMP
                     AYVYGKAQLDELMKE"
     gene            8181..8822
                     /locus_tag="PKM87_02760"
     CDS             8181..8822
                     /locus_tag="PKM87_02760"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNQ29018.1"
                     /translation="MCGEFSSMPILGEPAPDFEALTTQGMVKLSDFKGKWLVLFSHPA
                     DFTPVCTTEFIAFSAIAEDLKSLNVQLMGLSIDSVHSHLAWIRNVKEKMGVDIPFPII
                     ADLDMKVAKKYGMIHPGQSSTAAIRAVFFIDDKGILRAMIYYPLTAGRFMPEIVRLVK
                     SLQTTDKYGVATPANWQPGDKVVVPAPKNKEEMDRRLSEGYECKDWYLCFKKI"
     gene            8915..9529
                     /locus_tag="PKM87_02765"
     CDS             8915..9529
                     /locus_tag="PKM87_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNQ29019.1"
                     /translation="MEIKVLAFAGSPRRGGNSETLLDWVLSSMAEEDGVVIEKIPLTE
                     ANVNPCRGCNACETLNRCVQRDGMDVLHDKIIEADCIILAAPIFCMGICAQAKALIDR
                     FQVFRSRKYVLKLPVVPPERKGKRLGIFLSTAGQNWGHVFDGAVPSVKCFFHVIDVRD
                     ADIHYLMVNNVDEKGAILQHPTAQADATRIGKEVITEVKKRLGG"
     gene            9533..10150
                     /locus_tag="PKM87_02770"
     CDS             9533..10150
                     /locus_tag="PKM87_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNQ29020.1"
                     /translation="MPVSVLGLSGSPHRHGNTETLLDAFLEGADSAGGQVEKIILKDL
                     DYHPCRGCNACHKTGECVVKDDAIPLFNRILAAECLAVASPIYSMGITAELKGLIDRA
                     QFMWARKFILKNLYFPNEQIRRHKGIFISTAGLSWDNVFDAAFPAVTAFFNTLGFEYY
                     DNIIANDMDRYQGIRGHPTAIAEAKKKGTEVVLLLEKMKEEEAST"
     gene            complement(10155..10532)
                     /locus_tag="PKM87_02775"
     CDS             complement(10155..10532)
                     /locus_tag="PKM87_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29021.1"
                     /translation="MTRWSDTAAIPSRADSETLSVAFTLVFRQGRAPPSCPSPREAEL
                     LNQICDRVQAASPAACRDALIRVRKLSYDVYIVCDEFREGIFGTGDEAQAAAINALAE
                     INPGFSKEEYRTAFVTGMMWTAF"
     gene            complement(10673..11845)
                     /locus_tag="PKM87_02780"
     CDS             complement(10673..11845)
                     /locus_tag="PKM87_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ29022.1"
                     /translation="MSRIILEARDIRYRYPRGMEAIRGISFHVRRGEKIALVGPNGAG
                     KSTILLMFNGMIRPDSGTILFDNVPISYDRASLRTLRKSVGFVLQNPDRQIISPTVYQ
                     DVAFGPTNLGFTDEEVKEAVEGALGRVGLSGFGRRPPHHLSGGEKKRVAIAGVLAMDP
                     DVLVFDEPTSGLDPSGSEDIMELLDELNQQGKTVIISTHDIELAYPWADRAILLLNGR
                     ILQEDVPDVAFGNPELVRKARLSMPTVLELYLELGRRGFCLPERRPKTVLDMLNVIDR
                     AFRNKPCYTQPGSITLFDIDSSHDTDIPGWCAAHPGVPVGAMGTRAKQRAADEKIWLD
                     FTYGVIDKCILKALLGEHSLIIVTGSMVGRVHERVREFCRENQVEIQVSSLSKRHG"
     gene            complement(11857..12645)
                     /gene="cbiQ"
                     /locus_tag="PKM87_02785"
     CDS             complement(11857..12645)
                     /gene="cbiQ"
                     /locus_tag="PKM87_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HNQ29023.1"
                     /translation="MYEELLEDIAQQNRLREVNTYTKLAAGLGAILLCLLSSSYVAPL
                     FIALVFAGALIFIARIDPAIYAKAFVAPLLFAGLGAGAIILLAGGGEIFWSWQIFPWL
                     SLSVTRESINQGVFVFFRVVGGTSALLFIAFSTPMTDLFQVMRRARVPEEVLDLAMII
                     YRTIFLIMDHLVMVYQAQMMRLGYRTFRESIRSFATLAGAVFIASWEAGEDLTRAMEA
                     RCYEGKFAVLGEGRPFSLPSVLAVISFLFMSAGVVAATTHVTLI"
     gene            complement(12674..12979)
                     /locus_tag="PKM87_02790"
     CDS             complement(12674..12979)
                     /locus_tag="PKM87_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011450133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transport protein CbiN"
                     /protein_id="HNQ29024.1"
                     /translation="MWAKYRLEIIAVAAIVCFCGVFLYTSSLNADAEYAGSDTLGSSR
                     AAELAGISEEDFQPLVPQWEPPSGEIESGLFALQAAVGGIIVGWVFGFWRGQKNRST"
     gene            complement(12979..13671)
                     /locus_tag="PKM87_02795"
     CDS             complement(12979..13671)
                     /locus_tag="PKM87_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HNQ29025.1"
                     /translation="MHIMEGFLPLQWAVFWWVLALPCLAFGVLQLKRILGQDREALPL
                     LGVTGGFIFILSSLKLPSVTGSCSHPTGTGISSVCFGPWVTSVICAIVLLFQSLFLAH
                     GGLSVLGANIISMGVVGPLAGWAIYRALRDTGVNMYFVVFFACAIADLCTYIVTSLEL
                     ALAYPAEVGGFAGSFVLFMGIFAITQVPLAIVEGVVLALVFKYIVHLRPEILIRLKVF
                     PEEKIRAAAGDA"
     gene            complement(13772..14398)
                     /locus_tag="PKM87_02800"
     CDS             complement(13772..14398)
                     /locus_tag="PKM87_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50"
                     /protein_id="HNQ29026.1"
                     /translation="MLERITRRERSDLLIAWLAISIAFSLIFIRGGVTVELFVIYLLV
                     SMFTVGIGFILHEMAHKFTAMKYGFWAEFRKDNLMLLVAVAMAALVGVVFAAPGATVI
                     YGTYISREQNGKISAAGPAVNILLCIPFAFILFVSGMFPVSFLSRNLLALVGMVGLQV
                     NAMIAAFNMLPVSVLDGKKVLAWNPAVFAVLIIAAFGILIASFYPSFF"
     gene            14501..14785
                     /locus_tag="PKM87_02805"
     CDS             14501..14785
                     /locus_tag="PKM87_02805"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNQ29027.1"
                     /translation="MPLDALRSEINRIDEQIIDLIAERQHLAARMAQVKMNEGLPVHD
                     VKRTHEVLDRAFNYAVEKNINPVSVKHIFALLIEMSEEKQRECQGDGNLP"
     gene            complement(14791..15972)
                     /locus_tag="PKM87_02810"
     CDS             complement(14791..15972)
                     /locus_tag="PKM87_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNQ29028.1"
                     /translation="MGTTRPEVRKKRLSNLCKLVISLIFVTLFLANAWADSPGPAPEG
                     GEKRESPIPSTLLYEDGKPVVHFFYNTYCTECQKTVPYMQEIAPLYPGVVFRFHDIRE
                     SDEARILFQEFKQQYGREFLPVPSLFLGPYVLSGYEEITANLGDAIRLTMMQGEVRPT
                     IPVPEVTGTKEVLTIPLVVGAALVDGINPCAFAVLIFLVLSLIALDTRKRVVTVGATF
                     ILAVFCFYFLSGLGLFAIVQSSGMSLLISVAAAAIAILAGVISIGEALWKRKTPFLSI
                     PESTRGIIDTWAKKGSVPGAFVLGVLVGMFELPCTGGIYLAILSLLSNRMTMYEGIPY
                     LLLYNLIFVFPLVLILAVVALGIAPEKLEKLREEKRPIIRLAMGGVMILLGIVLILEI
                     I"
     gene            complement(15977..16849)
                     /gene="argB"
                     /locus_tag="PKM87_02815"
     CDS             complement(15977..16849)
                     /gene="argB"
                     /locus_tag="PKM87_02815"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNQ29029.1"
                     /translation="MKREEVLTEALPYIQQFHGKTMVIKLGGHAMVDAAILETAIRDA
                     VLLQLVGIRVVLVHGGGPEITDKMKAMGKEPKFVAGLRITDEETLEIAQMVLVGKINS
                     NIVSLIAKSGGKGVGLSGNDGNLIIARRMDPQKVHVAGREQEVDLGHVGEIEEINPAV
                     LFTLLDRGYIPVVAPIAIDRSGQSLNINADTAAGEIAISLEAYKLINMTDVDGIMDAS
                     RTTVYRRLRVNEAEQLMGSGVISEGMIPKVSSLVRAVNSGVSFGHVVNGNIAHNLLLE
                     MFTDEGVGTMICAR"
     gene            complement(16846..17997)
                     /gene="argJ"
                     /locus_tag="PKM87_02820"
     CDS             complement(16846..17997)
                     /gene="argJ"
                     /locus_tag="PKM87_02820"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HNQ29030.1"
                     /translation="MKSICGVEGVTAWGLKEGKFGLALIRAEGEAAGVFTSNLMKAPP
                     VSLMEERIRGGVLSAVMANSGCANAYTGARGYRDAAEMAEICAEVMHVTPEEVGVAST
                     GVIGRYLDLPLIRDQCKRVAPNLARSEEAEVRAARAIMTTDITEKHALVRCENFSVGG
                     ITKGSGMIAPNMGTMLAFIYTDAEVPAPALREALKAAARRTFNRVVVDGDMSTNDIAL
                     CTATGKAGRVNPREFSQALEECCRMLAIQIAEDGEGASKLLEVTVKGAPGEEEAATVA
                     KTVVGSPLVKTAVYGADPNWGRVIAAAGRAGVEFDPDCVNLWMGNGTESVPLVESGEI
                     VVDLARAKALMKGKKVLFVLDLAAGDGEATAWGCDLTEKYVEINGRYTT"
     gene            complement(18014..18487)
                     /locus_tag="PKM87_02825"
     CDS             complement(18014..18487)
                     /locus_tag="PKM87_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNQ29031.1"
                     /translation="MLIREVMTKNPVTVQGDAPVRDAAALLRAHKIGGLPVMDGEKLV
                     GMITESDILRLLETGKISDDLWLPSPLEVIEVPIREIINWEKTKHALASIGNIQVRKV
                     MSHPAITVREDEEIEDAASVMLSKKIARLPVVRGETLVGIVARADIVRGVAAPGQ"
     gene            complement(18506..19492)
                     /gene="argC"
                     /locus_tag="PKM87_02830"
     CDS             complement(18506..19492)
                     /gene="argC"
                     /locus_tag="PKM87_02830"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNQ29032.1"
                     /translation="MDIAIIGASGYAGGDLIRLLLGHSRARVSCVTSRKLAGKPVESI
                     HPQFKGLLDLEFSNPSPKDLEADVAFLAVPHTAAMAYARPLLSRGIKVIDLSADYRLP
                     KEIFEKVYGVPHTDYFTAPYGIPELRRDSYRDADFVSNPGCFPTGATLAAAPLARYAH
                     TVIYDSKTGVSGAGDNPSATTHYPNVGDNVNPYKWTSHRHLAEMKQELVSLGSHAKCY
                     FTPHLVPVNRGILTTAHILLKEPIGQEEVELLYRNYYEGEFFVRLQNPVLAAVRGTNF
                     CDIRVESEGDRVVAVSAIDNLGKGASGQAIQNMNIICGFDERDGLTGAGLLP"
     gene            19957..21858
                     /gene="acs"
                     /locus_tag="PKM87_02835"
     CDS             19957..21858
                     /gene="acs"
                     /locus_tag="PKM87_02835"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNQ29033.1"
                     /translation="MAESFEVPLSGDEKTYIPDPSYKQNAWTKDYTTAYKEFMADPDG
                     FWEKQAHHFEWIRKWDRVLEWNYPYAKWFVNAKLNITANCLDRHVNNHRRNKVALIWR
                     GEEGEERTFTYKKLFGEVCRFANGLKKLGVAKGDRVCIYMPLVPEQVIAMLACARIGA
                     VHSVVFGGFGVQALNMRIKDAEAKIVITADVTYRRGKTIPLKTIVEEAVINAPSVEKV
                     VVLSREVEQPVEIHKEMEVDYFELMEGVPTTCEAEVMDAEDPLFILYTSGSTGTPKGI
                     VHTCAGYMVGTAYTTQHVFDVKDNDVYWCTADTGWITGHSYVVYGPLAIGATIVLAEA
                     VPDYPDAGAFWKIVEDLGVTIFYTAPTAIRMFMKVGEQWPNKYDLSSLRILGSVGEPL
                     NPEAFEWYYQVIGKKKCPIVDTWWQTETGMQMITTMVGEPMRPGFAGRPIPGVVADVV
                     DKKGKPVAPGNGGFLVIQRPWPAMLRTVYKNDERYRTYWNTIPDCYTAGDLAVKAKGD
                     EYIMIIGRADDIIIVAGHNIGTAEVESALVSHQSVAEAAVIGKPEPMKGNIIKAFVIL
                     RVGFSPSEKLKSDLIYHVRMTLGPIAMPSEIEFVDKLPKTRSGKIMRRVLKAQEMGID
                     PGDVSTLEE"
     gene            21919..23145
                     /locus_tag="PKM87_02840"
     CDS             21919..23145
                     /locus_tag="PKM87_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HNQ29034.1"
                     /translation="MPAEKGRWVLVFLGMAINLCLGSIYSWSVFVNPLTDYFTGSLGQ
                     SVTANEVLMPFSVFLAFFAIAMPLAGKYIETLGPRNVTIIGGVLTGLGWLLASMVTSV
                     QMLYLVYGVIGGVGVGIAYGVPVAVAARWFPDRRGLAVGLTVLGFGFSAFLTANIAGY
                     LISSFGVMDTFRIFGIAFVVLIIVLALPLRFPVPGWKPRGWNPPVPKPGEETCECDRG
                     AMLRTPTFYGLWACFFIGCLAGLMAISIAKPVGTEIGIEAGLATLLVGFFAVFNGGGR
                     PIFGSLTDRITPRNTALASFVLIALASLALWQVPAIPVYILAFAILWGCLGGWLAIAP
                     TATGTYFGTCDYPRCYGLVFLAYGAGAIAGPQLAGFIKTTTGNYMGVFPYVLALSLIG
                     LAIAWVLVKAPKKMTS"
     gene            23258..23875
                     /locus_tag="PKM87_02845"
     CDS             23258..23875
                     /locus_tag="PKM87_02845"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNQ29035.1"
                     /translation="MEEVEKSRLEALSDGIFAFAMTLLVISLAVPEIPYEQAPRVLPV
                     LIAGMAPEFLLFVIAFFILAGYWISHHRILEQVKYVDGVLVRINILLLFFIVLIPFTT
                     SISGDYTNVLEAVLFFHVNLLLASFTLSGMGWYIHRHMATLAPGMQPPWKSGLEKAVV
                     MHGVILLAIGVSFMNTRASMWCYALIPIIYFVLGAIHRILEAEKR"
     gene            23872..24627
                     /locus_tag="PKM87_02850"
     CDS             23872..24627
                     /locus_tag="PKM87_02850"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ29036.1"
                     /translation="MKGLDILYRIHSGLPRQGPGSTECTARAFRHVNLQNMHPLVLDI
                     GCGSGASALVLSRLTGGTVVAVDNHQPFLVELKERAWALQGPGRKEVQPLQASMSSLP
                     FLNGSFDLIWSEGAAYLMGFEEALLSWRPLLKREGFLVVSEVTWLECNPPEEIRSYWE
                     ANYPAIASDSSHIETIARSGYVLHRSFTLPESTWWNYYRPLQGKMRKFRREYADDPVA
                     QSVLDEIDEEIGMYENYHGSYGYRMYVMEKRDD"
     gene            24620..25144
                     /locus_tag="PKM87_02855"
     CDS             24620..25144
                     /locus_tag="PKM87_02855"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HNQ29037.1"
                     /translation="MIDRRKEAIRESGLPPLIGPSPEVLVLGSFPSRMSLAAGRYYAN
                     PRNHFWPIMQQVLSLEGEGIPEWEEGLKSAGVAVWDVIASRRFQPGSMDRDICDEEWN
                     DIPGLLVTHPTIRCICLNGGKAKESFRKSMRAVPLPQPTAVHPLPSTSPANARQTLYK
                     KIETWQVLRQYLRD"
     gene            complement(25177..25479)
                     /gene="eif1A"
                     /locus_tag="PKM87_02860"
     CDS             complement(25177..25479)
                     /gene="eif1A"
                     /locus_tag="PKM87_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HNQ29038.1"
                     /translation="MVDETAPPGMETIRVRLPKKRNREQFAIAELMLGSNHIKVRCID
                     GVTRMGRIKGKIKKRAWIREGDTLIITPWSFQDEKCDISYRYTKPQVDWLRRNRYL"
     gene            25698..26480
                     /locus_tag="PKM87_02865"
     CDS             25698..26480
                     /locus_tag="PKM87_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29039.1"
                     /translation="MQLRVFFSLALLFLVGCLAFSGCLTSPPVEEPIQETPTTVPTAI
                     PATAGPLTETATPTPTPTAIGLSSEDINLHFMDVAFGGGNLFLERLPLSSKKTTISLN
                     AGTEPDKIVVESFATRFNVLSQSNKLFENIKEGQTGDIRIKFLPPAGLKAIDLTSEMG
                     WLNEEYTCNGVTCAKIKGYDIYLNAEMEGDQRVHYLLRSLLYTLGFKGASLRYPDSIF
                     AYPDNAATELSFLDEKALQVMYGLGMDNGMTVDDVKRVLFFR"
     gene            26550..27182
                     /locus_tag="PKM87_02870"
     CDS             26550..27182
                     /locus_tag="PKM87_02870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ29040.1"
                     /translation="MAPDMDDIYRKIPPGKIPWNIQSPPDILVDLVESRTITPCNTID
                     LGCGAGNHAVYLAGRGFRVTGVDMSPAAIEMARKNAEKRGVLCTFIVADVLDDLGGVG
                     GPFQFAYDWELLHHIFPPDRDRYVRNVSNLLVSGGMYLSVSFHEDDPHFGGSGKYRRT
                     PIGTTLYFSSGEELFDLFSPHFTIIESKVRTIPGTSGTHEANCFLMVKVS"
     gene            27284..27955
                     /locus_tag="PKM87_02875"
     CDS             27284..27955
                     /locus_tag="PKM87_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNQ29041.1"
                     /translation="MKVLGLNASPRGKESRTLRLVEAVLKGAAAEGAETDLVDVYSLR
                     IEYCTACTSCYASGECIISDDFPLLFEKMMCADGIVLGAPNYIDSVPAPMKAVFDRMA
                     DAIHCRMFTGKYGCAVCTSGGPNHGPIVEYMNHALSSLGAITVGGVGVAVGRGLEAIP
                     AAEEDSRSLGRILVRAIRGELRYPDQEEIQQQRRDYFRQLILWNRDRWTHEYDWYARM
                     GWLDE"
     gene            27984..28754
                     /locus_tag="PKM87_02880"
     CDS             27984..28754
                     /locus_tag="PKM87_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HNQ29042.1"
                     /translation="MDISLRDRFIGLWQEYFPGAELPVTFELTEDDRGVERAPAPNGW
                     RCIVCDLARVRRGKSLVFTQESLSCSGAKYYMGFVKGRAPDFRYFLSCGKPGSVRGER
                     YKKNPEIVDELVQKGTFLPAGSRHYLFSRWDNLNPDDNPDVVIFFARAEVIAGLFTLA
                     NFDRAEPHGVICPFGSGCSSIIHYPLHEGMAECPRAVLGMFDPSARPCVPVDMLTMAI
                     PMRLFGQIVESMEESFLITDTWEKVKKRIEMSEPRNPR"
     gene            complement(28863..30071)
                     /locus_tag="PKM87_02885"
     CDS             complement(28863..30071)
                     /locus_tag="PKM87_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ29043.1"
                     /translation="MRGIILQLSLRNIRIHFLRSVLAALGIVIGVVAISSMGMMGANM
                     TLSVTEELSGMANNLVVRADTGGGGFGGGPGGGGGSGNGDESISEEEFREITKIAEKY
                     GLVYGVQSESDRIEVGDRRGRSTVFGLDDTVIPTLFTLSEGSFPKSTSSVVIGPTLAN
                     RFDLKTGSRIDIGDPEGGVTTTVRVVGILEERGMSMDINSDSAIIANQKLFNGIYGKE
                     GEYDQVNIILNDINDTARVKSDILDKMNRKEETVTIQDSSRMLESITSTLSTMTTFVM
                     AIAGISLLVAATSIFNVMMMSVTERVREIGIMRSIGTQKSEVRKMFIYEAVILGAIGA
                     VIGAVLSLAIGWIVVLAMVGSTDYFFSPASLVYIPSAMVVGIVICVASGVYPAWRASN
                     LDPIEALRAE"
     gene            complement(30068..30742)
                     /locus_tag="PKM87_02890"
     CDS             complement(30068..30742)
                     /locus_tag="PKM87_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ29044.1"
                     /translation="MDEKPLIELAGVTKVYPLPSGDVVALDRVSLTIEDGEFVAIMGP
                     SGSGKSTLLNHLGCLDRPTSGDLYIRGRNTREMNDCELTELRLNTIGYIFQRFNLIPL
                     LTAYENVEYPFLLKHRKPDDTGWVDSLLGMVGIDRAMAAHKPPELSGGQQQRVAIARA
                     LVNDPMLLLCDEPTGNLDSVTGHQIMEVLSELNEKGRTILMVTHDPEVAKCARRTIVM
                     RDGRVA"
     gene            complement(30750..32027)
                     /locus_tag="PKM87_02895"
     CDS             complement(30750..32027)
                     /locus_tag="PKM87_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29045.1"
                     /translation="MKKKNGMIPSRMTGIILLLAISCIAATVLAATSAEDAASYVSVN
                     KVTVEPATFMPDDAGTITVDISNGGTAPVAIYRAEILSQDIKVLNYQTYDSVGAIGPG
                     TTMQFTFRIKPEVEDGMYFPMFYLDFTDAGSLRYPVPLEVDSTPVTVSVVSAPASFSS
                     GTKEQVVLSVSNPRNNAVNSVTAIPGGDGIRSTQSSVFIGTLEPDEEKKATFEITAER
                     DTTMAFDVSYRNGNNLHHLVLEMPVTVGEGGTAAEMVVNNVEVVQAGSTFTLSGDVTN
                     AGLRDAKSVKVTVDSPAVPVDPNPVYVIGALEPDDFSSFEVTCTVRDVSSIPLIIQYR
                     DKDGKVFEDRVNISIRSTAVPASGEASGASQGLPSGNTRRPGGMLGFGNGISKIPFTE
                     ILIVIAACIAVAVAWRKGYLGKAVDIVRKRSGR"
     gene            complement(32360..32557)
                     /locus_tag="PKM87_02900"
     CDS             complement(32360..32557)
                     /locus_tag="PKM87_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNQ29046.1"
                     /translation="MKNKIKVFRAMHDLTQEALAQEMGVARQTILAIEKGKYDPSLDL
                     AFKIARRFGVSVDEMFTCWEQ"
     gene            complement(32547..33002)
                     /locus_tag="PKM87_02905"
     CDS             complement(32547..33002)
                     /locus_tag="PKM87_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2178 domain-containing protein"
                     /protein_id="HNQ29047.1"
                     /translation="MKQNTFFLMAGLIGLIEVGIFWLSVLLKESMLITIAFVVGILVI
                     YIARRTISDKRDDERSALITQKAGARTLEVFWILFFAVSLGSLVMGFATPLGIQPPPK
                     PFPRAPPDEPHIGYFGLLQMILLCCMAFLYIGFRIYYARKYGEWDDDEE"
     gene            complement(33128..34321)
                     /locus_tag="PKM87_02910"
     CDS             complement(33128..34321)
                     /locus_tag="PKM87_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNQ29048.1"
                     /translation="MHDHVPARELQDRMSRFLARMDDDSQGWEMVAIFGKINLYYFTG
                     TIQDGVLLVGRDKDAVFWVRRSYDRAIEESNFPDIRPMRSYRDAALDTVSHPNRIYLE
                     TEVVPLALAERFRKHFPFSEVSPMDLQAARVRSIKSPYELALLEKAGEIHRIVLEEEA
                     PKVLRQGMSEAEFGTAIYSLMVERGHQGVVRFGRFNTEIEVGQIGFGESSLYPTCFDG
                     PGGCYGAYPGAPVLGSHTRKLRSGDLVFIDNSCGVEGYQTDKTMNYMFGRPVPEYAIE
                     IHSRCVELQDEIASLLKPGVAPSTIYTTIMAGLDQEFLTNFMGFGERTVQFLGHGVGL
                     VVDEIPVIAMGFDEPLQERMVIAVEPKKGIPGVGMVGTENTYVVTPDGGRSITGKCRG
                     LIPVY"
     gene            34448..34828
                     /locus_tag="PKM87_02915"
     CDS             34448..34828
                     /locus_tag="PKM87_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HNQ29049.1"
                     /translation="MLDIFKCEKCGKIVMVVSDGDGQLVCCGMPMKRQDENTVDASNE
                     KHVPVVEPHGKEILVRVGSVPHPMEPAHHLQWIEVMSGPMLYVKGLKPGEKPEAVFPV
                     STTDVKVRTYCNLHGLWSNKPHHR"
     gene            34974..35798
                     /locus_tag="PKM87_02920"
     CDS             34974..35798
                     /locus_tag="PKM87_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ29050.1"
                     /translation="MKMERPGTGSHADTPPLLEFAHVTVMKGEKKLLDNLTVCIRDGE
                     HVAILGPNGSGKSSFIRTVNREYYPVLGTDGEVIFRVCGRDVWDVFDLRGLFGIVSPD
                     LQYIYTRNVTGREVILSGFFSSVGLFNHHITPEMEERADQIIQFLEVGHLVNRRMDAM
                     SSGEARRFLIGRALVHNPRALILDEPTNSLDLHALHTFRQALRKIAGSGVGIIMATHN
                     LHDIIPDITRVILMRDGGFFMDGPKEDVLTDANIGDLFRVPFRIREEGGWYYASGY"
     gene            complement(35846..36085)
                     /locus_tag="PKM87_02925"
     CDS             complement(35846..36085)
                     /locus_tag="PKM87_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HNQ29051.1"
                     /translation="MRKDPSFHYRAPEESRKDGRNRSGIFPGAMVRIVKKQDQGTGRL
                     TEGKVKEVLTNSTFHPHGIKVRLEDGSVGRVREFC"
     gene            36228..37328
                     /locus_tag="PKM87_02930"
     CDS             36228..37328
                     /locus_tag="PKM87_02930"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNQ29052.1"
                     /translation="MTHMQFTFFALLFAVALAGVVTADVTMQAGAAGSGSVYTTGNIY
                     VSSAPAGASAILDGGHSQLFTAGTFRGVEPGTHQVLITLQGFQPYTTTVKVSAGATGN
                     VIATLPPVTSPGGISVSSTPRGAGVYVDDIYMGKTNQVVGNLAAGPHRVVVSEAGYVT
                     WEETVQVRSKEVIPVAVTLVAEVNAASGDLLVSSTPTGASVYIDGNYRGSTPVDDSLD
                     INDLSPGTYTISVKKPGYQEYATTITLQAGRKVQVFAPLTPAEQTAASVEISSTPAGA
                     DVFVNNLYMGITPLSFHDVSAGTYTVDIRMEGYVPFSSSGQVIPGQSVHVIAALSPVQ
                     TPAPTTKAPTGALAVVMAFVMTGIVCLLVARR"
     gene            complement(37276..37662)
                     /locus_tag="PKM87_02935"
     CDS             complement(37276..37662)
                     /locus_tag="PKM87_02935"
                     /inference="COORDINATES: protein motif:HMM:NF020549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1894 domain-containing protein"
                     /protein_id="HNQ29053.1"
                     /translation="MSPEPCFNRIWSDIILKDATPRRADNRVRKACREHYLITPGYVF
                     RGVTILIERPMLVGIDEEAGRILMPFRKPCYGTSLYAIESDRVEIAALREALSVAEPE
                     NPGKKIVDSSISLQPGGRQSRSLQKP"
     gene            37868..38290
                     /locus_tag="PKM87_02940"
     CDS             37868..38290
                     /locus_tag="PKM87_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29054.1"
                     /translation="MFAQKPTVFKSRAWITVMFIFGIVLIGIGMMNLAFLAGEESPGL
                     ISMFISAGFVLTILSGFRLWKGETNYMQDERTKRIGAYGLSWSWFLTFLFLFGIFWAD
                     YLHLWSPDAQTLSVLLILFMGISAKGFQAYLFRKGDVD"
     gene            38310..38531
                     /locus_tag="PKM87_02945"
     CDS             38310..38531
                     /locus_tag="PKM87_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNQ29055.1"
                     /translation="MQTRIKEFRAKRALTQAELADLVGVRRETIVFLEAGKYNPSLRL
                     AHQVAKELGTTIDTLFIFGDEGHYTVTEE"
     gene            complement(38582..>39060)
                     /locus_tag="PKM87_02950"
     CDS             complement(38582..>39060)
                     /locus_tag="PKM87_02950"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ29056.1"
                     /translation="HLRFAQDYQEFGSNQPAWQDLGHMVAHAIEDVPHEGIGVMPTLE
                     RVEINADPLARRVFSNLLDNAMRHGEDVSSVRIWTKEGPAGELILVFEDDGVGIPDNE
                     KEMIFRYGYGKHTGIGLAISRDILSLTGITIRETGKAGKGARFEVHIPPRAWRRPE"
BASE COUNT         8415 a        11360 c        10897 g         8388 t
ORIGIN      
        1 cttccctcgc ggtcagtaga gcatggccgg cctgcaccgg taaaacctga attcatcgtg
       61 cctgtaaaag agaacagcga gaaggcagct cgtgatgcca tggaggggct gtaccggatc
      121 gtgtttgatt gagaggtggg gacctcggga tgtcccggaa agatcttatg cagtggaatc
      181 cgggggaatg catacactgc tctttccagg tctaaaaaaa gaatcaagtg tggtctgcct
      241 cgcccggaac tgtgacttca gtaccgtgct ctcgtttacg aacctcccca tgggaagagt
      301 cattcgcatc gatacatccg cgagagccga ggggaggtct tcgaattcac ggaacggctg
      361 gtgcatcgcc tgcctcacgt tctccctgac attgaagacc cccatgggga tatatccctt
      421 gcgggcctcc cgaaggacga tcgccccagc ctgcaccccc tccttctcga ggccctccag
      481 cactgccatc ttgcaggagt agtagcagcc ccccacgcag gaatacccct tcttcccacg
      541 gaactcctcg taatctgaaa agagcagctc ctcgtttccc ctgatatgga ggaatgcctc
      601 catccactcg tactgccagg gggtggggag gaggatgacc gcgtagtgat tgttgaggct
      661 cgagaactcg tggacccgta tcgtgtcgat caggcggtta tgacggaccc tggaaaggaa
      721 ccggtcggcg agagtcgtgt cgcaggcggt aatcgaccac cgggtgggga ccatcctcct
      781 ctttcctccc gtcccaagcg atcccaccga gagagccttc tggatgctgc tgaagggaac
      841 cccatccctg tggagagtga ggatagcctc agatgcaggg agatcagaat cgtagtagca
      901 ccgctccagg tgtggctcga accggctgct ttcggcctcg aattcgagca ggtgggcgct
      961 cgggccaaac ggggtgtgct cttcagagaa agaggtcccc gccggggtct ccgagaataa
     1021 cgcctggctc tcgattgatc ccgccgagag agcaatctcc tgcagccggc aggcatcagg
     1081 gtcatcgaca cgggtgacat cgcagacccg ctttcccctc accaggtcga ggcggcatga
     1141 gaggatctcc tcctgtgacc accctgccgg gatccatgcc tccggggtgt ccatgatccc
     1201 acactcctcg cgaaccggcg cgattagcgg gccggcgtag accttcgggt agttccagct
     1261 cccgataaag actgatgggg gcgtgcttcc ctccatcgtc ctgtcaagaa tgcgggatga
     1321 gccaagcctc gacgaagtga tggccttgag gtacgcgcct ttcccgatgg ccatgtcaga
     1381 tgagtattcc aggtatatcc agataaagtt gcacggtgca gggagaaagt gatgccacta
     1441 tacgcccatc agggtgagaa gtatgcagat gcagaccggg atggtgaggt tgtcgtccac
     1501 gggagagacc agttccaccg caccggcggc gaccgaggct gcgattgcag ggaggggcgg
     1561 gaggaaaaat acgagcacca ggatgttgac gagcacccct cctgccgagc cctccacaga
     1621 tttcccgttc gtgatccggt gcctccccca ccgcagtccc acgagcgtgg ctgcgccgtc
     1681 aaggaccgcg agcgagagta ttgcagggac cgaaatttcc gcatcgaaga agagtacgca
     1741 gaagagggcg cttgcggcga agtagaaagc ccctttcccg gggtattcgc cgtcgcgctc
     1801 aaggttgcgg atgagaaggg aaacgagcgg gatacgatat ccccgcaaca ccccttcggc
     1861 gaggatgaac ccgataaata ttgaggccgc gagaaggagg actatcaggt cttttgggag
     1921 gagatatgca agtgcggcaa ttcccactcc gaagaacatg tggacaatct tccgggacgg
     1981 ctcgtgcatg cccagagtat gggtatcccg gaccattaac tggtcggatt gcgatccttc
     2041 atcccgggtg gagaggttga tgagcaaaag gggctcaata ctacatgcaa tgacaggggc
     2101 aggcgacctt cggttcaggg gagccggaga tgtcatcacc gaatcccttt cggtatatgg
     2161 aatccaggtt atacgcagca ccggcgagtt ctggaccgcg aggcagcggc agggttcatc
     2221 cctccacgag gtctcctacc gcggctgctt caagccgcag ctgcccgcct acttcatcaa
     2281 cctcctgacg aaaccaggtg acctggtata cgaccccttc agcgggaggg ggaccaccgt
     2341 catcgaggcc gggttgctgg ggagggctgt tgcggcaaac gacgccaatc cactctcgcg
     2401 cctcctcacc cgcccccgtt tctttctgcc tgatactgcc gatgtgaagt caaggctcga
     2461 ggcgataccc attgataccg gtgcgggggc cgagatggac ctctccatgt tctttcaccc
     2521 cgggacagag gcggagatct cctctcttcg caactacctg ctcgagagag agagggacgg
     2581 ggacatcgac catgtcgacg attggatacg catggtcgcc accaacaggc tgacaggcca
     2641 ttcaagcggc ttcttctcag tctacacgct cccgccgaac caggcagtct cacccatgcg
     2701 gcagatacgg atcaaccaga agagggacca ggtaccgcca taccgggaca caagagctct
     2761 gatcctgaaa aagacccttt ccctcctgaa ggacgtgagc gaaatccaga aggaacgcct
     2821 gcggaaatcg ggggagaatg ccctcttcct ttcgggcgac tcgcggcgga ctccggggat
     2881 cggtgacggc accgtccagc ttaccatcac ctctcctccg ttccttgacg tggtccagta
     2941 ctcgtccgac aactggctcc gttgctggtt caatgggata gaccccggaa ctgtggaaaa
     3001 aaacctcacc gtcacccgct cactccccga ctggaggaaa gtaatgcagc agacgctgta
     3061 cgaactcttc cgcgtcacga ggagcggggg ccatattgcc ttcgaggtcg gtgaagtaaa
     3121 gggggggact gtcagactcg acgagacggt ggtgccgctc ggtgtccgcg ccgggttctc
     3181 gtgcgagggg atactcgtga acctgcagga cttcaccaag acatcccaca tttggggcat
     3241 cgggaataac gcccatggga ccaacaccaa caggatagtg gtcttcaaaa aagaggggtg
     3301 agatggttca ctccagtttt accaggtaat cgtaggcctc gagcgccgcc tttgccccct
     3361 ctccggcggc agtgatgatc tgcttggtcc tgatattcgt cacgtcaccg gccgcaaaga
     3421 ctccgggaac gctcgtgtgg cagttgatgt cgatctcgat ctccttctgg ccgttgagac
     3481 gcaggaatcc ttcgaggaaa tcggtgttcg gtatccatcc cacctccgca aagaccccgt
     3541 cgagggaaat tgtggtctcc ttgccggact cgcggtcctt tagcgtgatg ccgttgagca
     3601 tggcgttccc gtgcagcgct gcgaccgtgt ggtgaaggta agtgcggatg tttccctgct
     3661 gctcgtactg cttcacgtag gcctcgtctg cctttatcgt gctcctgacg acaaggtgga
     3721 cctcgcgtgc aatcttgctc atctcgatcg cggtctggag ggccgagttc ccgccgccga
     3781 ccacggcgac caccttgtcc cggaagagcg ggccgtcgca ggttgagcag acggaaaggc
     3841 cgcggcccag gtatctctcc tccccttcca ccccgagttt cctgggacgc ttcccctggg
     3901 caaggatgac tgtcttggtg gaataggtgc ctcccgttgc ggtatcaacc acgaagaggt
     3961 ccccttccct cctgatcgcc tggatccggt caagttcgag gtgtatgtgc tctcccctga
     4021 cctgctcctc gaacttgtgc atcaggtctt cccctgtgac catgcgatac cccatgtaat
     4081 tctcgatcgc ccagctctca agggcctgtc ccccgatgtt ctcagagatg atgactgtct
     4141 ttaccatctt cctcgagcag tagacacctg ccgtgagccc tgcgggcccc gcaccgacaa
     4201 tgagtacgtc gtagatgtct ccttcccttg cggtcccgaa gagctcgttc aaccgctggg
     4261 catcaaatcc cacgatgacc tcatcgtcga cggtcgtgac cgggactccg tactgtccgg
     4321 agagctccac catcttcccg gcctgctccc ggtcggtccc tacgtcgatt gccatgtaat
     4381 cgacgctgtg cttttccagg aaagccttca gcatgcggca gtaggggcag ttctgggtat
     4441 agtagacggt gaccctgggc atgatggggg gtttgccggg gatgagtaat aaacgtggtg
     4501 gagcggggtg tgtagctgat tcctgtcacc tgaatgagat gccctgacag aatacccggt
     4561 gagaagagga gtcgtcccat ccctcattcg cccgggggat tttggaaaat ggggttgtag
     4621 gaaaatcagt actgggggtt gatcggctgg aatggcatat ccttgtcaaa caccctgtag
     4681 gtaaccccgt tgaccatagt cacgtatacg attacccggt cggtcctggt cgtgccggtg
     4741 agcacgatct ctgatcccat ctgcgggctt tgcacagtct gctggtcgac cgttccatct
     4801 gacctgatca cttccgctgt catggattgc gtgaatgcca gcccctggcc tccttcatac
     4861 gttaccgtga tggtgggatt ggtggcgatg gtgttcttta ccacctgcac tgtcaccgag
     4921 tatgtggggg gcatgatgtc ggtaggctcg gggacgaggg acaacgtggg cgtacgaggg
     4981 ggcatagtcg tcggtgcagg tgaggctgtc gtgatggggg aaccgggggc attcccaagg
     5041 cagcctgctg caagaacaag gacacatgca aggattagaa gagagatgcg caagatcatc
     5101 atgggaatat ctctgctcca ggttctatat caatacacaa gaagcctatg cccgcaggca
     5161 tgggacctcc tctctcctcc gcaaaagaca gaacgcaaga gaaaatgaat ggggttattc
     5221 aaccgtcctg aacacttttc ggactgccat gcagatcggg cacttttccg gggccgtccc
     5281 aaggaagaca ttgccgcaga cagggcagat gtacaccgtg cggttggaca tatcctggcc
     5341 gtggttcagg ttattgagcg cctccttgta gagcgttgca tggacctcct cagccttcat
     5401 cgcgtaggtg aagacaagcc cgatgtcgct ttttttctcg gactcagcct ccttcaggaa
     5461 cgcggggtac atctccttga actcgtgggt ctcccccgaa accgattctg ccaggttctc
     5521 tttggtggcg tggatctttc ccatcgcctt gaggagcttc ttcgcgtgga tcgcctcggc
     5581 ttcagaggcg gcccggaaga ggcgggcaat gttcttgtac ccttcggatt ctgcctggtc
     5641 tgcaaacgcg gcatacttac ggttggcctg cgactcacct gcaaaggcct ccatcgcatt
     5701 ctccatggtt gccatggtgg tatgtttgag agagggcgcc ttaaaagtag gtattctccc
     5761 cagccaggag caggagatat cccggggcca cccggtatat tcttgtcctg gcacatgcct
     5821 gcaaccccaa attatttcga acagcagccc tggcttttgg gtatgaatgc gtgccatttt
     5881 tctggttccc ctgcacatgc ttcccctgcg atcccagatg aaacggatga gggaactcga
     5941 caggtgcgac aggccccgtg agaagatcgc gaaaaagggg gcacgctcac tctcggacca
     6001 ggagctgatt gccgccatca tcggaaacgg catgcagggc cgcgatgtct tccaggtggc
     6061 aagggagatt gccaggctct ttcgggaaag cgcggataca atcacctaca atgacctgac
     6121 ctccatacgc ggggtcgggg aagggaaggc atcccagatc ctcgcgtcga tggaactcgc
     6181 ccggcgatac ctcaaaaatg aggaagaggc cagggtaaag atccaggcgc ccgccgacat
     6241 cctccctctc gtcgccgggc tgaacgggca gcgccaggag catttcatct gcgtcacctt
     6301 aaacggtgca cacgaggtga ttgccacccg gaccatcacc gtcggcctcc tgaaccacag
     6361 ccttgttcat ccccgcgagg tgttcgcgga cgccatcacc gaccgggcgg ccgcggtgat
     6421 ctgcgtgcat aaccaccctt cggggactct tgagccaagt tcccaggacc tggccatcac
     6481 tcgacaactg agggatgccg gcgatctcct cggtatcagg cttctcgatc atctcattgt
     6541 cgcacggact ggctacctga gcttaaaaga gcagggacat ctctgatcag gcgctccctt
     6601 ccacgagcag ccgacccatc tcatagccct ttttgtatgc cgactccaga aggtccggtc
     6661 ttgtggtgat atcctggatg gtgtccatgt caggtacgag aagatcatcc cagtattgca
     6721 cgtcgattat ggagaagaat gcgctcatcg tcatcttcgc accgtcaaag acattcgcaa
     6781 ccttcatccc ggaaatcccg aggtagagcc cccggcggtg ggcacgacgc tcctttggga
     6841 ccagggcttt tttccgcatg tattttgcca tgaaaaagac ctggaaccgg tccatgaagg
     6901 acttcaatgc cccggggatc cccatcgtca tgacgggggt cgcaacgatc aggctgtcca
     6961 gttcacggaa tttctcatag atggcagtca tgtcatcctt catggcgcag gtctcgtgct
     7021 ccctgcagta gaatatctcc ctgcagggcc ggaacgcgag gtgtgcgacc tcgatcttct
     7081 ccactccgca accggcatcg gaagcgccct taagcgctgc atccaggagt ttcgcggtgt
     7141 ttccatgagg gagcgggctt ccgagcagcc cgatgatgcg gtatcccata cagttaggtg
     7201 gcagggcccc cgcatatatc ttcggattgt tgcccttata tttggcagca tccaggtata
     7261 caggtgcgtg ttaccccaaa tatcggggtt tgatggggaa gaacaaaagc cataaataat
     7321 gacttgaacc atataccgag ttggtgatag acctgaccga gaaggcaaaa gccgggcaga
     7381 aagttggccc cggcaaatac gtctgtctgg actgcggacg ggaactgacg ctcgacgctg
     7441 tcgagcagga cttgagaaaa tgcccgtcat gctcctgtga agagtaccag tgtttcccga
     7501 tgacacacat ccgcccggac atcaagaccc cggaagatgt gatgaacccg ccgaagcgcg
     7561 gaaagagcaa ccagtaactt tcattttttt cttcacagcg ggagcaagtc cagggtatcg
     7621 tcttcagaat atggatggtt cccggatgca tagtatttcc gggacttttt aagcttggag
     7681 ggggatcatg gtgaatgtat caaaggaagg acagcagttc gaatgtgaga tctgtggaaa
     7741 tgtcgtcgtg gtgaaggttg tcggcggggg agaactcgtc tgctgcggcc agcccatgaa
     7801 gctgaagaag tgatgcggct atggcaaaag agaccaaaaa tatcaaagac cttgagaaga
     7861 tgatcggcaa ggtcccgaag ttcttcaagg aacttgcaga gaaggacccc atgatgtacg
     7921 agatggtgat gaagctcgag gggcacatct gggacgacgg agcactcacc cggaagacaa
     7981 aaaagctcat tgcaatcgcc atcgccgcat cgcttcgcga ccagcatgcc acaagggcgc
     8041 agcttgcagg tgccgcaaac ctggggatta aaaagaacga ggtggaagaa gcattgagag
     8101 ttgcattcct cctggcaggc atgccagcct acgtatatgg aaaggcgcag ctcgatgaac
     8161 tgatgaagga gtgaattgaa atgtgcggtg aatttagttc aatgcccatt ctcggcgagc
     8221 cggcccctga ttttgaggcc ctcaccaccc agggcatggt aaagctctct gatttcaagg
     8281 ggaaatggct cgtgctcttc tcgcaccctg cggattttac cccggtgtgc actaccgagt
     8341 tcatcgcatt ctccgcaatc gcagaggatt taaagtcact caacgtccag ttaatgggcc
     8401 tctccatcga ctcggtccat tcccacctcg catggatcag gaacgtgaag gagaagatgg
     8461 gggtcgatat cccgttcccc atcattgccg accttgacat gaaggtcgcc aagaagtatg
     8521 ggatgattca tcccggccag agcagcactg ctgcaatcag ggcagtcttc tttatcgatg
     8581 acaaggggat actgcgggca atgatctatt acccgctgac tgccgggcgg ttcatgcctg
     8641 agatagtccg cctcgtcaag tccctccaga caaccgacaa gtacggggtc gcgacccctg
     8701 cgaactggca gcccggtgac aaggtggtgg tcccggcacc gaagaacaaa gaggagatgg
     8761 acaggcgtct ctccgagggg tacgagtgca aggactggta cctctgcttc aagaagattt
     8821 aaaatcctaa aaaactctca tttttccgcg gtcgcttctc ttatataccc ctgttcaaat
     8881 cattttttca tctccggtgt cataccggtg ttcaatggag atcaaggtcc ttgcgtttgc
     8941 cgggagcccg cggcggggcg ggaactccga gacgctcctc gactgggtcc tctcgtcaat
     9001 ggcagaggag gacggggtgg tcatcgaaaa aattcccctg acggaagcca atgtgaaccc
     9061 ctgcaggggc tgcaatgcct gcgagacgct gaaccggtgc gtgcagcgtg atgggatgga
     9121 cgtcctgcac gacaagatca tcgaggccga ttgcatcatt ctcgctgccc cgatcttctg
     9181 catggggata tgcgcccagg ccaaggcgct catcgaccgt ttccaggtct tccggtccag
     9241 gaaatacgtc ctcaagctcc ctgtcgtgcc gcccgaacga aaggggaagc gcctcgggat
     9301 attcctctcc accgccgggc agaactgggg ccatgtcttt gacggcgcgg tcccgagcgt
     9361 caagtgcttc ttccacgtca tcgatgtcag ggacgcagac atccactacc tgatggtgaa
     9421 caatgtcgat gagaagggtg ccatcctcca acaccccact gcacaggctg atgccacgag
     9481 gatcgggaag gaggttatca ccgaagtgaa gaagaggctc ggggggtaat tcatgccggt
     9541 cagcgtcctc ggcctgtccg gaagccccca caggcacggg aatacggaga ccctcctcga
     9601 cgccttcctc gagggcgcgg attcagcggg cgggcaggtg gagaagatca tcctcaagga
     9661 tcttgactac cacccctgca gggggtgcaa tgcctgccac aagacagggg agtgtgtcgt
     9721 gaaagacgac gcaatccctc tcttcaaccg gatccttgcg gccgaatgcc ttgcggtcgc
     9781 ctcccccatc tacagtatgg ggatcaccgc ggaactgaaa ggactcatcg atcgggcgca
     9841 gttcatgtgg gccaggaaat tcatcctgaa gaacctgtac tttccaaacg agcagatccg
     9901 ccggcacaag ggcatcttca tctccaccgc aggtctctcc tgggacaatg tctttgacgc
     9961 ggcattcccg gctgtcaccg catttttcaa cactctcggc ttcgagtact acgacaacat
    10021 catcgcaaac gacatggaca gatatcaggg aatccgggga cacccgaccg caatcgcaga
    10081 ggcgaagaag aagggaacgg aagttgtttt gctccttgaa aaaatgaagg aagaggaagc
    10141 gtcgacatga cctgtcagaa tgccgtccac atcattcctg ttacgaatgc cgtccggtac
    10201 tcctctttgg aaaaaccggg gttgatctca gcaagggcat tgatcgccgc tgcctgtgct
    10261 tcatccccgg tgccgaaaat cccttcccgg aattcatcac agacaatata cacgtcatag
    10321 gagagttttc tcacgcgaat gagtgcatcc ctgcacgcgg caggagatgc cgcctgaacc
    10381 ctgtcgcata tctggtttaa gagttctgct tcgcgggggc tcgggcacga gggaggagcc
    10441 cggccctgcc gaaagaccag ggtgaatgct acgctgagcg tctcgctgtc agccctcgaa
    10501 ggaatagcag ccgtatcaga ccacctcgtc actctctgct cccatagttg ccggcctggt
    10561 tcgaatcaac gtatggcaca cagcaggatt gacaaatatt ggcatctgat cgtatctcct
    10621 ttgcgaagat gagaactgtt gtcaacccat tgggtcctgc caggccgggg cgtcatccat
    10681 gcctttttga gagagaggag acctgaatct caacctggtt ctcccggcag aactcccgaa
    10741 ccctctcgtg gaccctcccc accatgctcc ctgtgacgat aatgaggctg tgctcaccga
    10801 gcagcgcctt caggatgcac ttgtcgatga ctccataggt gaagtcaagc catatcttct
    10861 cgtctgcagc ccgctgcttc gcacgggtcc ccattgcccc cacgggaacg cccggatgtg
    10921 cggcgcacca tcccgggata tccgtatcgt gtgaagagtc gatgtcaaag agggtgatgc
    10981 tgcccggctg tgtgtagcag ggcttgttcc tgaaagcccg gtcgatgacg ttgagcatat
    11041 cgagcaccgt ctttggcctg cgctccggga ggcaaaaacc ccttcggccc agttccaggt
    11101 atagttcgag gaccgtgggc atggagaggc gggctttccg cacgagttcc gggtttccaa
    11161 aagcgacatc cgggacgtct tcctggagga tccgcccatt caggaggagg atggcacggt
    11221 cggcccaggg ataggcgagc tcgatgtcgt gtgtcgagat gatgaccgtt ttcccctgct
    11281 ggttcagctc gtcaaggagc tccatgatgt cctctgaccc cgacgggtcg aggccgcttg
    11341 tcggctcgtc gaacaccagc acatccgggt ccatcgcgag cacgcctgca atggccacac
    11401 gcttcttttc tcctcctgat aggtggtggg gagggcggcg cccgaaccca gagaggccca
    11461 cacgccccag ggcgccctcc accgcctcct ttacctcttc gtcagtgaac ccaaggttgg
    11521 tcggaccgaa tgcgacgtcc tggtataccg tgggggagat gatctgccgg tcaggattct
    11581 ggagcacgaa ccccacgctc ttccggagcg tcctgagaga tgcccggtcg tacgaaatgg
    11641 gcacgttgtc aaagagaatg gtccctgaat cgggacgaat catgccattg aacatcagga
    11701 ggattgtgga cttcccggca ccgtttggac cgaccagtgc gatcttctcc cccctccgga
    11761 catggaagct gatcccgcga atcgcctcca ttcccctggg ataacggtac cggatgtccc
    11821 ttgcctcaag gatgattctg ctcattttgg tgactcctat atcaacgtga catgggttgt
    11881 ggccgcaacg actcctgcag acatgaaaag aaacgagatg accgcaagga cggacggaag
    11941 ggagaatggt ctcccttctc ccagcactgc aaatttcccc tcgtagcaac gggcctccat
    12001 tgcccgggtc aggtcttccc ccgcctccca gctggcgata aagactgcac ccgcaagggt
    12061 ggcaaacgag cgtatcgatt cgcggaatgt gcggtacccg aggcgcatca tctgtgcctg
    12121 gtagaccatc accaggtggt ccatgatcag gaagatagta cggtagatga tcatggcaag
    12181 gtcaagcact tcctcgggaa cccgtgcacg ccgcatcacc tggaagagat ccgtcatggg
    12241 ggtggaaaac gcgataaaga ggagggcgga agtgccacca acgacgcgga agaagacaaa
    12301 caccccctgg ttgatgctct cccgtgtgac agagagcgag agccatggaa agatctgcca
    12361 gctccagaag atctctccgc ctccagcaag gagaatgatt gcgcccgcgc ccaggcctgc
    12421 gaagaggagc ggcgcaacga aggcctttgc gtagatcgcg ggatcgatgc gggcgataaa
    12481 aatgagagct cccgcaaata cgagggcaat gaagaggggg gccacgtaac tcgaagagag
    12541 gaggcagagg aggattgccc cgagccccgc ggccagcttg gtataggtat tgacctccct
    12601 cagcctgttc tgctgggcga tgtcctcaag gagctcttca tacatggagc actgcacata
    12661 gatgttggtc ccttcaggtg cttctattct tctggcctct ccagaacccg aacacccatc
    12721 ccacgatgat tccacctacc gctgcctgga gcgcaaagag accggactcg atctcaccgc
    12781 tcggcggctc ccactgcggg acaaggggct ggaaatcctc ctccgagatg cctgcaagtt
    12841 ccgcggccct tgatgacccc agggtgtcgg aaccggcata ttcggcatcg gcattcaggg
    12901 aacttgtata gaggaagacc ccgcagaagc agacgatagc tgcaactgcg atgatctcga
    12961 gacggtactt cgcccacatc aggcatcacc tgctgctgcc ctgatctttt cctcggggaa
    13021 caccttcagg cggatgagga tctcgggcct gagatggacg atgtacttga agaccagggc
    13081 gagcacgacc ccttccacga tcgcaagcgg cacctgggtg atagcaaaga tacccatgaa
    13141 caagacgaag gaccctgcaa acccacccac ttcggcagga tatgccagtg cgagctccag
    13201 tgacgtcacg atatacgtgc agaggtcggc aatggcacag gcaaagaaca ctacaaaata
    13261 catgtttact cctgtatctc gaagggcacg gtagatcgcc catccggcga ggggaccgac
    13321 gacccccatg gagatgatgt ttgcaccaag taccgagagg cctccatggg ccaggaagag
    13381 gctctggaag aggagcacta tcgcgcagat gaccgatgtg acccacggcc caaagcagac
    13441 cgaagagatg cctgttccgg tcggatgcga acagcttccc gtgaccgaag ggagtttcaa
    13501 ggacgaaagg atgaagatga agcctcccgt cacccccagc aggggcaggg cttcgcggtc
    13561 ctggccgagg atcctcttca actggaggac gccgaacgca aggcacggga gggcgagcac
    13621 ccaccagaac accgcccatt ggagagggag aaagccttcc atgatatgca tgatcagatc
    13681 accttaaaca atttaatttg atttagaaca aattaatctg attaatattt atgccttccc
    13741 ttttcaccga ccggtgaaaa ggaaaaaagg ttcagaaaaa ggagggataa aaagatgcga
    13801 tgagaatgcc gaaggcggca atgatgagga ccgcgaatac cgcggggttc cacgcgagca
    13861 ctttctttcc gtcaagcacg ctcaccggga gcatgttgaa cgcggcgatc atcgcattca
    13921 cctgaagccc caccatccca acgagcgcaa ggaggttcct cgaaaggaag gaaacaggga
    13981 acatcccgga cacgaagagg atgaacgcaa acgggatgca gaggaggata ttgaccgcag
    14041 gccctgccgc ggatatcttc ccgttctgct ccctgctgat gtaggttccg tagatcactg
    14101 tggccccggg tgctgcaaag acgactccga caagggctgc catcgccacc gccacgagga
    14161 gcatcaggtt atccttccga aactcggccc agaacccgta cttcatagcc gtgaacttgt
    14221 gcgccatctc gtggaggatg aacccgatcc ccacagtgaa catggagacc agaaggtaga
    14281 tcacgaagag ctccaccgtg accccacccc tgataaagat cagggaaaac gcgatcgata
    14341 tggcgagcca tgcgatgagc aggtcggacc gttcccggcg ggtgattcgt tcgagcatgg
    14401 ttcttgtccc ttcattgtat ggtcttcctc ccccttatca cctttggatg gggcgcgtgc
    14461 caccaccttt aaatcccttc actccctgaa ggtagagtgt atgcccctcg acgcactcag
    14521 gagtgagatc aaccggatcg acgagcagat catcgacctc atcgcggaaa ggcagcacct
    14581 ggcggcacgc atggcgcagg tgaagatgaa cgagggactg cctgtccacg acgtgaagag
    14641 gacccacgag gtgctggacc gggccttcaa ttatgcggtt gagaagaata tcaacccagt
    14701 ctctgtcaag catatctttg ccctcctgat cgagatgagc gaggagaagc agcgggagtg
    14761 ccagggggac gggaaccttc cctgaattga tcagatgatc tcgaggatga ggactattcc
    14821 aaggagtatc atcactcccc ccatcgcaag cctgattatc gggcgcttct cctcccgcag
    14881 cttctccagc ttttcggggg cgatcccgag cgcgaccacg gcaaggatca gcaccagggg
    14941 aaatacgaag atcaggttgt agaggagaag ataggggatt ccctcgtaca ttgtcatcct
    15001 gttcgagagg agcgagagga ttgcaaggta gatccccccg gtgcagggca gctcgaacat
    15061 ccccacaagc actcccagca caaatgctcc cggcacggac cccttcttcg cccacgtatc
    15121 gattatcccc cttgtcgact cggggataga gaggaatgga gtttttctct tccacaatgc
    15181 ctcccctatg cttatcaccc ctgccaggat tgcgatcgcc gctgccgcca cagagatgag
    15241 aagagacatc ccggatgact ggacgattgc gaagagaccc aggcccgaga ggaagtagaa
    15301 gcagaagacc gccaggatga aggttgcccc caccgtcacc accctcttcc tggtatcaag
    15361 ggcgataaga gagaggacga gaaatatcag caccgcaaac gcacaggggt tgattccatc
    15421 cacgagcgcc gcgccgacaa ccagcggaat tgtaagcacc tctttggtcc cggtgacttc
    15481 gggaacggga atggtcggac gaacctcccc ctgcatcatg gtaagcctga tggcatcgcc
    15541 gaggttggcc gtgatctcct cgtacccgga gaggacatag gggcccagga agagcgaagg
    15601 aaccggcagg aactcccggc cgtactgctg cttgaactcc tggaagagga tccgggcctc
    15661 gtcgctctcc cggatgtcgt ggaaccggaa gacgaccccg ggatagaggg gagcgatctc
    15721 ctgcatgtag gggacggttt tctggcattc agtgcagtat gtgttgtaga agaagtggac
    15781 taccggcttt ccatcctcgt aaaggagggt ggaaggaatc ggcgactctc ttttctcccc
    15841 tccctcaggg gccgggccgg gagaatccgc ccaggcattc gcgaggaata gagtgacgaa
    15901 tatgagggaa ataaccagtt tacagaggtt tgataacctt tttttgcgca cttccggcct
    15961 cgtcgtgccc atctccctag cgggcgcaga tcatggtccc cacgccctcg tcagtgaaca
    16021 tctcgaggag gaggttgtgg gcgatgttgc cattcactac gtgcccgaac gagacaccgc
    16081 tgttcaccgc tctcacgagg gagctgacct ttggaatcat cccttccgag atcacgccac
    16141 tccccatcag ttgctcggcc tcattgaccc ggagcctcct gtagaccgtg gtcctcgagg
    16201 catccatgat gccgtccaca tcggtcatgt tgatcagctt gtaggcctca agcgagatag
    16261 caatctcgcc tgccgcggta tcggcgttga tgttcaggct ctggccgctc cggtcgatag
    16321 cgatgggcgc caccaccggg atgtatcccc ggtcaaggag cgtgaagaga acggccgggt
    16381 tgatctcctc gatctccccc acgtgcccga ggtcgacctc ctgttccctg cctgcgacat
    16441 ggaccttctg ggggtccatc ctcctcgcga tgatgaggtt cccgtcgttg ccagagagcc
    16501 ccaccccttt tcctccgctc tttgcaatga gggaaacgat gttgctgttt atctttccta
    16561 cgaggaccat ctgggcgatc tcaagggtct cctcatcggt gatgcgaaga ccggccacga
    16621 acttgggctc ttttcccatt gccttcatct tgtcggtaat ctctggaccc ccgccgtgaa
    16681 ccaggacgac gcggatgcct accagctgca ggagcaccgc atcccttatg gcagtctcga
    16741 ggattgccgc atccaccatc gcatgccccc ccagcttgat caccatcgtc tttccatgga
    16801 actgctggat ataagggagg gcttcagtca ggacttcctc tcgtttcatg tcgtatacct
    16861 cccattgatc tccacatact tctccgtcag atcgcagccc cacgccgtgg cctccccatc
    16921 tcccgcggcg agatccagga cgaagaggac cttttttccc ttcatgaggg cttttgcacg
    16981 ggcgaggtcg acgacgatct cgccgctttc caccagcggc actgattccg tgccgtttcc
    17041 catccagagg ttcacgcaat ccgggtcaaa ctccacgcct gccctccccg ccgcggcaat
    17101 tacccgtccc cagttgggat cggcgccgta caccgcggtc ttcacgaggg gtgacccgac
    17161 caccgtcttt gccaccgtgg ccgcctcctc ctctcccggg gctcccttca ccgtgacctc
    17221 caggagcttg cttgcaccct ctccatcttc agcgatctgt atggcgagca tccggcagca
    17281 ctcctcgagg gcctgcgaga actcgcgtgg attgacgcgt cctgcctttc ctgtcgcggt
    17341 gcagagcgca atatcattgg tgctcatgtc gccgtccacc accacgcggt tgaacgtccg
    17401 cctggctgca gccttgagcg cctcacgaag tgcgggggcg ggcacctccg cgtcggtgta
    17461 gatgaatgcg agcatcgtcc ccatgttggg ggcaatcatc ccagaaccct ttgtgatccc
    17521 gccgaccgag aagttctcgc acctgacgag cgcgtgtttc tcggtgatat cagttgtcat
    17581 gatggctcgg gcggcacgca cctcggcttc ttcgctcctg gcaaggttcg gggcgaccct
    17641 tttgcactgg tcccgtatga ggggaaggtc gaggtagcgc cctatcactc cggtactggc
    17701 cactcccacc tcctcagggg tgacgtgcat cacctctgca cagatctcgg ccatctccgc
    17761 ggcatcccgg tagcccctgg cccctgtgta tgcattcgcg cacccgctgt ttgccatgac
    17821 cgccgagagc acgcccccgc ggatacgctc ctccatcaaa ctaaccggcg gggccttcat
    17881 gaggttcgaa gtaaatactc ctgccgcctc gccttcagcc cttatcaggg caagaccaaa
    17941 ctttccctcc ttcaagcccc acgctgtcac cccttccacg ccgcagatgc tcttcattca
    18001 gtgactccct ccattactgg cccggagccg ccactccccg gacgatatcc gcccgggcca
    18061 cgatcccgac gagggtctca cccctgacca cgggaagacg cgcgatcttc ttcgagagca
    18121 tcaccgacgc agcgtcctcg atctcctcgt cctcgcgaac ggtgattgcg gggtgactca
    18181 tcaccttcct gacctggata ttcccgatgg atgcaagagc gtgcttcgtc ttctcccagt
    18241 tgatgatctc cctgatcggc acctcgatga cctcaagcgg ggaaggaagc cacagatcgt
    18301 ctgagatctt ccctgtctcg agcagcctga gaatgtcgga ctccgtgatc attcccacca
    18361 gcttctcgcc gtccattaca gggagacccc caatcttgtg tgcacggagc agcgcggctg
    18421 cgtcccggac cggggcgtcc ccctggaccg tgaccggatt ctttgtcata acctctcgta
    18481 tcagcattct tttcattcac ctcgttcagg gaagcagccc tgcacccgtg aggccgtcac
    18541 gttcgtcaaa gccgcagatg atgttcatgt tctggattgc ctggccgctc gcacccttgc
    18601 caaggttgtc gatggccgat accgccacca cccggtcacc ctcgctctcc accctgatat
    18661 cgcagaagtt ggtgcctctg accgctgcaa gtaccgggtt ctgaagcctg acaaagaact
    18721 ccccctcgta gtagttccgg tagagaagct cgacctcctc ctgccctatc ggctctttta
    18781 gcaggatatg ggcggtagtg aggattcccc ggtttaccgg gaccaggtgc ggagtgaagt
    18841 agcactttgc atgggaaccg aggcttacga gctcctgctt catctctgca aggtgccggt
    18901 gggaggtcca cttgtagggg ttcacgttgt cccccacgtt cgggtagtgg gtcgtggccg
    18961 aagggttgtc cccggctcca gagactccgg tcttggaatc gtatatcacc gtgtgggcat
    19021 agcgtgcgag cggcgcggcg gcgagggtgg ctccagtggg gaagcatccc gggttgctga
    19081 caaaatcggc atcccggtaa gaatccctgc gcagttccgg gataccgtag ggagcggtga
    19141 aataatcggt atgcggcact ccatagacct tctcaaagat ctcttttggg agccggtagt
    19201 cggcagagag gtcgataacc ttgatcccgc gggagaggag gggccttgcg taggccatgg
    19261 ccgcggtgtg ggggaccgcc aggaatgcaa catcggcttc aaggtctttt ggggaaggat
    19321 ttgaaaattc caggtccagc aggcccttga actggggatg gatggattcg acaggctttc
    19381 ccgccagttt ccgggatgtc acgcatgaga cgcgtgccct ggaatggccg agcagcaggc
    19441 ggatcaagtc gccgccggcg tagccggagg ccccgatgat ggcaatatcc atacatctcg
    19501 tttcgatttg cagatgttaa tcctttttcc cttcattttc accggtattt gcctcttgcg
    19561 cgaccccgtg ccccagattc cgggtcccgc ggaaatgccc ggagtcagga acccggcccg
    19621 gcacggagcc atgttgcccc ctttcccgga catgtgtaga aaatgaacct ggaaaatggc
    19681 agtttttatt gcactgcaaa atcaccaggt tttaataatt ataatgatta atcattaaaa
    19741 tagcgcgtgg gggcatgtga agagaacgtg aagaaaaagg cgtgattttg gtttccgggc
    19801 ggtttttccg cccgtggtag cgtgaagcgg gtgaacactc gaacttataa tcacggagtg
    19861 tacattagag gaggtttctg gcgggagaaa ggctcccgcc tgcttctcac ataacaatgg
    19921 ctgattgaat gagtccaaac ggaaaggtgc aatacaatgg cagaatcatt tgaggtgccg
    19981 ctctcagggg atgagaagac ctacatccct gacccatcct acaaacagaa tgcatggacg
    20041 aaagactata ccaccgcata caaggaattc atggcagatc ccgatgggtt ctgggagaag
    20101 caggcccacc actttgagtg gatcaggaag tgggaccgcg tccttgagtg gaactatccc
    20161 tatgcaaagt ggttcgtcaa cgccaagctc aatatcaccg caaactgcct tgaccgccat
    20221 gtcaacaacc accgcaggaa caaggtggcc ctcatctgga ggggtgagga gggtgaggag
    20281 cggaccttca cctacaagaa gctcttcggg gaggtctgcc ggtttgcaaa cggcctcaag
    20341 aagctgggcg tggcaaaggg ggacagggtc tgcatctaca tgcccctcgt ccccgagcag
    20401 gtgatcgcga tgctcgcatg cgcccgcatc ggcgcagtgc acagcgtggt cttcggagga
    20461 ttcggggtgc aggcactcaa catgcgcatc aaggacgccg aggccaagat tgtcattacc
    20521 gcagatgtca cgtaccgccg tgggaagacc attccgttga agaccatcgt agaagaagcc
    20581 gttatcaacg ccccgagtgt ggagaaggtc gtggtgctct cccgcgaggt cgagcagccc
    20641 gtggaaattc acaaggagat ggaggtggac tatttcgagc tgatggaagg agtgcctacc
    20701 acctgcgagg ccgaagtgat ggatgccgag gaccccctct tcatcctcta taccagcgga
    20761 tccacgggga ctcccaaggg aatagtccac acctgtgcag gatacatggt cgggaccgcc
    20821 tataccaccc agcacgtctt tgatgtcaag gacaacgacg tctactggtg taccgccgac
    20881 accgggtgga tcacggggca tagttacgtc gtgtatgggc cgctcgccat aggagcaacc
    20941 attgtcctcg ccgaagcagt gcctgactat cccgacgccg gggcattctg gaagattgtc
    21001 gaagaccttg gggtcactat cttctacacc gctcccacgg ctatccgcat gttcatgaag
    21061 gtgggggagc agtggcccaa caagtacgac ctctcatccc tgcggatcct gggatcggtc
    21121 ggagagccgc tgaaccccga agcattcgaa tggtattacc aggttatcgg gaagaaaaaa
    21181 tgccccatcg tggatacctg gtggcagacc gagaccggga tgcaaatgat caccaccatg
    21241 gtgggagaac cgatgcgccc cggttttgcg gggagaccga tcccaggtgt ggttgcggat
    21301 gtcgtggaca agaagggcaa acccgtcgca cccggtaacg gcgggttcct tgtgatccag
    21361 aggccatggc cggccatgct ccggacggtc tacaagaacg atgaacgtta ccgcacctac
    21421 tggaacacca ttccggactg ctacaccgcc ggcgacctcg cagtgaaggc aaagggcgat
    21481 gagtatatca tgatcatcgg gagagcagac gacatcatca tcgtggcagg ccataatatc
    21541 ggaaccgcgg aggtggagag cgcactggtc tcccaccagt ccgttgccga ggcggcagtg
    21601 atcggaaagc cagagcccat gaaggggaac atcatcaagg cattcgtgat cctgcgggtc
    21661 ggattctccc cgagcgagaa gctcaagtcc gacctcatct accacgtccg catgacactt
    21721 gggcccatcg ccatgccctc cgagatcgag ttcgtggaca agctcccgaa gacccgctcc
    21781 ggaaagatca tgcgccgggt cctgaaagcg caggaaatgg gaatagaccc cggggacgtc
    21841 tccacattgg aggaatgatt cccccctttt tccggatcga aaaggactgt tatgcctgac
    21901 gaggggagca tgttcggtat gcccgcggag aaagggcgct gggtccttgt attcctcggg
    21961 atggccatca acctctgcct tgggagcatc tactcctgga gcgtctttgt caaccccctg
    22021 actgactact ttaccgggag cctcgggcag tcggtcaccg cgaacgaggt gctgatgcct
    22081 ttctctgtct tcctggcctt ctttgccatc gcgatgcccc tggcagggaa gtacatcgag
    22141 acactcgggc caaggaacgt cacgattatc ggtggagtcc tcaccggcct cgggtggctg
    22201 cttgcttcga tggtcacctc tgtccagatg ctctacctgg tctacggggt gatcggggga
    22261 gtgggtgtcg ggatcgccta tggggtccct gttgccgtcg ccgcgaggtg gtttcccgac
    22321 aggcggggac ttgccgtcgg gcttacggtg ctcgggttcg ggttctctgc attcctgacc
    22381 gcaaacatcg ccggttacct catctcctcc tttggggtga tggacacttt ccgcatcttt
    22441 gggatcgcgt tcgtggtgct tatcatcgtc ctggccctcc ccctccggtt tcctgtgcca
    22501 gggtggaaac cccgcggctg gaatcccccg gtgccaaaac ccggagagga gacctgcgag
    22561 tgtgacaggg gtgcgatgct ccgcaccccc acgttttacg gcctctgggc ctgtttcttc
    22621 atcggctgcc tggcaggcct gatggcgatc tccattgcaa aacccgttgg gaccgagatc
    22681 gggattgagg cggggcttgc aacgctgctc gtcgggttct ttgctgtctt caacgggggc
    22741 ggccggccga tcttcgggag cctcaccgac cggatcaccc cgagaaacac ggcgcttgcc
    22801 tctttcgtcc tcatcgcact cgcatcgctt gcgctttggc aggtgccggc tatcccggtc
    22861 tacatccttg cgttcgccat cctgtgggga tgcctgggcg ggtggcttgc catcgccccg
    22921 acggccactg ggacgtactt cgggacctgc gattacccac ggtgctacgg cctggtcttc
    22981 ctcgcctacg gggcaggggc aattgccggc ccccagctcg cggggttcat caagacgacc
    23041 accgggaact acatgggggt ctttccttac gtgctcgcgc tctccctgat agggctcgcg
    23101 atcgcctggg tgcttgtgaa ggcgccgaag aaaatgactt cctgattcat tccttttttt
    23161 ccccctttga tcgttccctg ttccggcaca cccggattaa atagggggaa ttcctacctc
    23221 atatccagca gacaccaggc agcgggagag tcacgcgatg gaagaagtgg agaagtcaag
    23281 gcttgaggcg ctgagcgatg gcatctttgc ctttgcaatg accctgctcg tcatatcgct
    23341 ggcggttccc gaaatcccgt acgaacaggc accccgcgtc ttaccggtat tgattgcagg
    23401 aatggccccc gaattcctgc tcttcgtgat tgccttcttt atccttgcag gctactggat
    23461 ctcgcatcac cggatcctgg aacaggtgaa gtatgttgac ggggtgcttg tacggatcaa
    23521 catcctcctc ctcttcttca tcgtgctgat accattcacc acatcgatat caggagatta
    23581 taccaacgtc ctcgaggcgg tcctcttctt ccatgtcaac ctcctccttg caagcttcac
    23641 tctttcgggg atggggtggt acattcaccg ccatatggca acacttgctc caggaatgca
    23701 accaccatgg aaaagcggcc tggaaaaagc cgtcgtcatg catggggtga tacttcttgc
    23761 aatcggtgtg tcgttcatga acacccgcgc aagcatgtgg tgctatgcgc tcatccccat
    23821 aatctatttc gtgcttggag ccatccatcg tatcctggag gcggaaaaga gatgaaaggg
    23881 ctcgatatcc tgtaccggat ccattccggc cttccacgac aggggccggg aagcaccgag
    23941 tgcacggccc gtgcgttccg gcacgtcaat ctccagaaca tgcatccact cgtccttgac
    24001 atcgggtgcg gttccggtgc atcagccctc gtcctctcac gcctcacagg tggaaccgtg
    24061 gttgccgttg acaatcacca gcctttcctc gttgagctga aggagcgggc ctgggcgctc
    24121 cagggcccgg ggagaaaaga ggtgcagccg ttgcaggcct cgatgtcgtc cctcccattc
    24181 ctcaacggga gtttcgacct aatctggagc gagggggctg cgtatctcat gggatttgaa
    24241 gaggcgctct tgtcgtggcg gccccttctc aaaagagagg ggttccttgt cgtttctgag
    24301 gtgacatggc tcgagtgcaa tcctccagaa gagatccgct cttactggga ggcaaattat
    24361 ccggcaatag catccgattc ctcacacatc gagacgatag cccgttcggg atacgtcctc
    24421 catcgctcct tcacgctccc agaatcgacc tggtggaact attatcgccc cctacagggg
    24481 aagatgagga aattccgaag ggaatatgca gacgacccgg tcgcgcagtc ggtacttgac
    24541 gaaatcgacg aggagattgg aatgtacgag aactaccacg gttcgtatgg ctataggatg
    24601 tacgtgatgg agaagcggga tgattgaccg cagaaaagag gctatcaggg aatccgggct
    24661 tcctccactc ataggcccct caccggaagt ccttgtactg ggaagcttcc cgagcaggat
    24721 gtcccttgcc gcaggtcgtt actatgccaa cccaagaaac catttctggc caatcatgca
    24781 acaggtcctc tctcttgagg gcgaagggat accggaatgg gaggagggac taaaaagcgc
    24841 aggtgttgcg gtctgggatg tgatcgcctc ccgcaggttt cagccgggaa gcatggaccg
    24901 cgacatatgc gacgaggaat ggaacgatat tccgggcctc cttgtcacgc atcccacgat
    24961 ccggtgcatc tgtctgaatg gtgggaaggc aaaagagtcg ttcagaaagt caatgagggc
    25021 agtgccgctg ccacaaccaa cagccgtcca cccgctcccc tcgacaagcc ccgccaacgc
    25081 gcgccagaca ctttataaga agatcgagac gtggcaggta ctccggcagt acctccggga
    25141 ctgagtggta actgcacccc ggggaaaaag gaggccctag aggtaccggt ttctccgcag
    25201 ccagtcgacc tgcggcttcg tgtaccgata ggagatatcg cacttttcgt cctggaagct
    25261 ccacggggtg atgatgagcg tgtccccttc ccggatccag gcccgcttct tgatcttgcc
    25321 cttgatccga cccatgcggg tgaccccgtc aatacaccgg accttgatat ggtttgagcc
    25381 gagcatcagt tcggcgatag caaactgttc cctgtttcgt ttttttggca gtcgcacgcg
    25441 gatggtctcc attcccggcg gtgccgtttc atcaaccact acttcttcgt tcctgtcttc
    25501 tcttttataa ttactaatag gccagcaact ccactgggaa catttttttt atccccttgg
    25561 gtttagtatt ggcgttcctg ccttatacca gtgatcatgc gggccggctc ctgtatcaca
    25621 agggtattga tgctgggtga agggcgtttt tttcccgcaa tgccaaccat tatgcgtcct
    25681 cgtaatgaaa tttattcatg caattacggg tatttttttc cctggcatta ctcttcctgg
    25741 tgggctgcct ggcgttctcc ggttgcctta cttcaccccc cgttgaggag cccatccagg
    25801 agaccccgac aaccgtgccg accgccattc cggccactgc cggccccctc acagagaccg
    25861 caacacccac tcccaccccc actgcgatag ggctctcgag cgaggatata aacctgcact
    25921 tcatggacgt ggccttcgga ggaggaaacc ttttcctgga acggctcccc ctctcctcga
    25981 agaaaaccac catctccctg aatgcgggaa ccgaacctga taaaattgtt gtcgaatcgt
    26041 tcgcgacgag gttcaatgtc ctctcccagt caaacaagct cttcgagaat atcaaagagg
    26101 gacagaccgg tgacatccgc atcaaattcc tccctcccgc gggattgaag gcgatcgatc
    26161 tcacatccga aatggggtgg ctgaacgagg aatatacctg caatggtgtt acctgtgcca
    26221 agatcaaggg gtatgacatc tatctgaatg cagagatgga aggcgaccag cgtgtccact
    26281 acctgctcag atcgctcctc tatacgctcg ggttcaaggg ggcatccctc cggtacccgg
    26341 acagcatttt cgcctacccg gacaatgcgg cgaccgagct ctccttcctg gatgagaagg
    26401 ccctccaggt gatgtacggc ctgggcatgg acaacgggat gacggtcgat gatgtcaaga
    26461 gggttctttt cttcaggtga ccgctcctcc cattcttttt ggcatatgaa cccccgtctt
    26521 ctcgaggatg aggcgtctgc ctgacggaca tggctcctga catggacgat atctaccgga
    26581 agattcctcc cgggaagatc ccgtggaaca tccagtcgcc ccctgatatc ctggtcgacc
    26641 tggtcgaatc ccgcacgatt accccctgca acacgattga cctgggctgt ggggcgggca
    26701 atcatgcggt gtacctggca ggcaggggat ttcgggtcac tggggttgac atgtcgcctg
    26761 cggcaatcga gatggccagg aagaacgccg agaagcgggg agtattgtgt acttttattg
    26821 tcgcagatgt tctcgacgac ctgggaggtg tcgggggccc gttccagttt gcctacgact
    26881 gggagctcct gcaccacatc tttcctcccg acagggaccg ctatgtcagg aacgtctcaa
    26941 atcttcttgt ctccgggggc atgtacctat cggtcagttt ccacgaggac gaccctcatt
    27001 tcgggggatc aggaaaatat cgcaggacac caataggaac caccctttac ttctcctctg
    27061 gagaggaact ttttgatctc ttctccccgc acttcaccat cattgaatcg aaggtgcgta
    27121 ccatacccgg cacttcgggc actcatgagg cgaactgctt cctgatggtg aaggtctcgt
    27181 aaaaatccac cctgctgcct gcagacccca atatgcggaa accggcaggt ttatcctgat
    27241 cctctcccga ttcccatcac acacgaggta aaatagtatg gacatcaagg ttctcgggct
    27301 gaatgcaagc cccaggggaa aggagagcag gacgcttcgc ctggtggagg cggtgctgaa
    27361 gggtgccgcg gcagaaggcg cggagactga tctggtagac gtgtattcac tccgcatcga
    27421 gtactgcacc gcgtgcactt cctgttatgc gagcggggag tgtatcatct ccgatgattt
    27481 tcctctatta ttcgagaaga tgatgtgtgc ggatggaata gtgctggggg ctccgaatta
    27541 catcgattct gtccctgccc ccatgaaggc ggtctttgac cggatggccg atgcaatcca
    27601 ctgccggatg ttcaccggga agtacgggtg cgctgtctgc acatccggtg gtccgaacca
    27661 cggaccaatc gtggaatata tgaaccatgc actcagttcc cttggcgcca tcactgtcgg
    27721 gggcgtgggt gtcgcggtag gcaggggcct cgaggcaatt ccggcggcgg aggaggactc
    27781 gcgttccctc gggcgaattc tcgtccgggc gatcaggggc gaactccggt atcccgacca
    27841 ggaggagatt cagcaacagc ggagggacta tttccgccag ttgatcctct ggaacaggga
    27901 caggtggacc cacgagtacg actggtatgc caggatggga tggctggacg agtagtggcg
    27961 aaccctctcg cggcgtgatt cacatggaca tttcattgcg agaccggttt atcgggctct
    28021 ggcaggagta ttttcctggt gcagaacttc ctgtcacgtt cgaactcaca gaggacgaca
    28081 gaggggtcga gagagcccct gcgccaaatg gatggcgatg catagtatgc gaccttgcac
    28141 gggtgcgaag agggaaatct ctcgtgttca cccaggagtc cctctcatgc agcggtgcaa
    28201 agtattacat gggctttgta aagggaaggg cccctgactt tcgctacttt ctctcctgcg
    28261 gcaaacctgg atctgtgaga ggcgagcgat acaaaaaaaa cccggagatt gtcgacgaac
    28321 tcgtgcagaa gggaactttc ctgcctgcgg gatcacgcca ctacctcttt tctcggtggg
    28381 ataacctcaa ccccgatgac aaccctgatg tggtaatctt ctttgcaagg gcggaggtga
    28441 ttgctgggct cttcaccctc gcaaacttcg atcgcgcaga accgcacggc gtgatctgcc
    28501 cgtttggatc agggtgcagc tccatcatcc attatcccct ccatgagggg atggcagagt
    28561 gtccgagagc ggtgctcggg atgttcgatc catcggcgcg tccctgcgtt ccggtcgaca
    28621 tgctgacaat ggcaattccc atgcgcttgt ttgggcagat tgtcgaatcg atggaggaaa
    28681 gcttcctgat cactgataca tgggaaaagg tgaagaagag gatcgagatg agtgaaccga
    28741 ggaatccccg gtaaaaggga aatccctcct tccaagggat cctatctccc acgcctgatg
    28801 gcgcgcggat tgtctgcatc accaggtcgg caatgaatgg atatgaggga atcccttccc
    28861 actcattctg ccctcaatgc ttcgatggga tcgaggttcg atgcccgcca tgcgggatag
    28921 acgccggatg caacgcatat gacgatgccg accaccatgg cagacgggat gtataccagg
    28981 cttgcaggtg aaaagaagta gtcggtgctc cctaccatcg caaggacaac gatccacccg
    29041 atggcaaggc tgagcacggc gcctatgaca gccccgatcg cccccagtat caccgcttcg
    29101 tagatgaaca tcttcctgac ctcggacttc tgggtgccga tgctccgcat gatgccgatc
    29161 tccctgaccc tctctgtcac cgacatcatc atgacattga agatcgaagt cgctgcaaca
    29221 agaagtgaga ttcccgcgat cgccatcacg aaggtggtca tggtcgagag ggtcgaggtg
    29281 atgctctcca gcatcctgct gctgtcctgg atggtcaccg tctcctcctt cctgttcatc
    29341 ttgtcgagga tgtcgctctt taccctggca gtatcgttga tatcgttgag gatgatattg
    29401 acctggtcgt actctccttc cttcccgtaa atcccgttga agagcttctg gttcgcgatg
    29461 attgccgagt cggagttaat gtccatcgac atcccgcgct cttcgaggat ccccacaact
    29521 ctcaccgtgg tggtcacccc tccttctggg tcgccgatat cgatcctgct cccggttttt
    29581 aagtcgaacc ggttggcaag tgtcggccct atcacgacag aactcgtgct tttcgggaat
    29641 gatccctccg agagggtaaa aagcgtgggg atcaccgtgt cgtccagtcc aaagaccgtc
    29701 gacctccccc tcctgtctcc tacctctatc ctgtcagatt ccgattgaac tccgtataca
    29761 aggccgtatt tctccgctat tttcgtgatt tccctgaatt cttcctccga tattgattca
    29821 tccccattgc ctgaaccccc gccgccgcca gggccgcccc cgaatccgcc acccccggtg
    29881 tcagccctga ccacgaggtt gtttgccatc ccagaaagct cctctgtaac cgagagtgtc
    29941 atgttcgctc ccatcatgcc cattgatgag attgccacta caccaattac aatgccaagg
    30001 gccgcaagca ccgaccgcag gaagtggatg cggatatttc tcaaagagag ctgcaggatg
    30061 atgcccctca tgccactctc ccgtcccgca tgacgatagt ccgcctcgca cacttcgcca
    30121 cttcagggtc gtgagtgacc atgaggatgg tcctcccctt ctcgttcagt tcagaaagca
    30181 cctccataat ctggtgaccg gtcaccgagt cgaggttccc ggtcggctcg tcacagagga
    30241 gtagcatcgg gtcatttaca agggcacgtg caattgccac ccgctgctgc tgcccaccgg
    30301 atagttccgg gggtttatgg gcagccatcg cccggtctat tcccaccatg ccgaggagcg
    30361 agtccaccca gcccgtatca tcaggtttcc tgtgtttcaa aaggaaggga tactccacgt
    30421 tctcatacgc ggtgaggagg ggaatcaggt tgaatctctg gaatatgtag cctatggtgt
    30481 tcaggcggag ttccgtcaat tcacaatcat tcatctcgcg ggtattcctc ccgcgaatat
    30541 agaggtctcc gctcgtcggc ctgtcgaggc acccaagatg gttcaggagt gtagatttac
    30601 ccgagcctga cggtcccatg atggccacaa actctccatc ctcgatcgtc agggagaccc
    30661 ggtcgagtgc gaccacgtcc ccggacggaa gcgggtagac ctttgtcacc cctgcaagct
    30721 cgatgagggg cttttcatcc acggttatct catctcccgg aacgcttccg cacaatatcg
    30781 actgcttttc ccaggtatcc tttccgccag gcaaccgcaa ctgcaatgca tgcagcgatg
    30841 acgatgagga tctccgtgaa aggaatctta cttatgccgt tcccaaaacc gagcatccca
    30901 ccgggtctcc tcgtgttccc cgagggtagc ccctgcgatg caccggatgc ctcacctgaa
    30961 gcaggcactg cggtgcttcg aatggatata ttcacacggt cttcgaacac tttcccatcc
    31021 ttgtccctgt actggatgat caaaggtatc gaggatacat cgcggactgt gcaggtgacc
    31081 tcgaaactgg agaagtcatc cggttcgaga gcccctatca catagaccgg attggggtcg
    31141 accggcacag caggtgaatc caccgttact ttcactgatt ttgcatcgcg gagaccggca
    31201 ttcgtgacat caccacttaa cgtgaacgtg gaccccgcct gcaccacctc cacgttgttc
    31261 actaccatct ccgcagctgt cccgccctct ccaacagtca ccggcatttc gaggaccagg
    31321 tggtgaaggt tattgccatt cctgtaggag acgtcaaagg ccattgtcgt atccctctcc
    31381 gcggttattt caaatgtggc cttcttctcc tcatcgggct caagagtccc gatgaatacg
    31441 ctgctctggg tcgaccggat gccatcacca ccaggaatgg ccgtcacgct gttcactgca
    31501 ttgttacgag gattgctgac agacaggaca acctgttctt ttgtcccgga agagaatgat
    31561 gcaggtgcgc tcaccacgct cactgtgacg ggtgtggaat caacttccag ggggacaggg
    31621 taccgaagtg acccggcatc cgtgaagtcg agatagaaca tcgggaaata catcccatct
    31681 tcaacctcag gtttgatacg gaatgtgaac tgcatggtgg taccgggacc aatggctcct
    31741 acggaatcat atgtctggta gttcagcact ttgatatcct gcgagaggat ctctgccctg
    31801 taaatcgcaa ccggggctgt cccgccattg gagatatcca cagtaatggt gccggcatcg
    31861 tcaggcatga atgtcgccgg ctctactgtc accttgttga cagacacgta tgaggcggcg
    31921 tcctctgcgc ttgttgcagc caggacagtc gccgcaatac acgagattgc gagtaggagg
    31981 atgatacctg tcatcctgga tgggatcatt ccgttcttct ttttcatagt aattcccgcc
    32041 tggattcacg ctcttgccga ttatcgggac atacaactcc ccgcagcccg cacgttcatt
    32101 gggggaagac gcttgaggtt cccctgtttg cagtctatcc ggactctccg gaagagagtg
    32161 acgctattcc tcaatcttaa gtaaaaaata ttttacatta agttatttaa atataactat
    32221 tagtcagtct gcactgacac ttccggcaca cagaaaggcc atgcacccgg aatgatacac
    32281 catatacctc atagggtccc attcccagac gagtctccgg cgtcttttcc tctttttcga
    32341 atgaaacact tcgtggtttt cattgttccc agcacgtgaa catttcatcg acacttacgc
    32401 caaagcgccg ggcgatttta aaggcaagat cgagcgaggg gtcgtacttc cccttctcta
    32461 tggcaagaat tgtctgcctc gcaacgccca tctcctgggc cagtgcttcc tgagtgaggt
    32521 catgcatggc ccggaacacc ttgatcttat tcttcatcgt cgtcccattc tccgtatttc
    32581 ctcgcataat aaattcggaa accgatgtac aggaatgcca tgcagcagag gagaatcatc
    32641 tggagcagcc cgaaataccc gatgtggggt tcatcaggag gtgctctggg aaagggcttg
    32701 ggagggggct ggatgccgag aggggtcgca aagcccatca ccagactgcc caggctgacg
    32761 gcaaaaaaga gtatccagaa tacttccaga gtcctcgccc cggccttctg cgtgatgagc
    32821 gcggaacgtt cgtcatccct cttatctgaa atggtcctcc ttgcaatgta aatcaccagg
    32881 attcccacta caaacgcaat cgtaatcaac atcgattctt tgagaagaac cgacagccag
    32941 aagatcccca cctcgataag gccgatcagg cctgccatga gaaaaaaggt gttctgcttc
    33001 attctatgta aatataatat tacattaagt aataaaaact atactatgtt cgtacccctt
    33061 cggcatattg aggcaggcgc aggcttgaga gcagagggga tttaaaaaac cggttctgga
    33121 acgggcatca atataccggg atgagtcccc tgcatttacc cgtgatgctc cggccaccat
    33181 ccggtgtcac gacataggtg ttctcggtgc cgaccatccc cacccccggg atccccttct
    33241 ttggctcaac cgcgatgacc atgcgctcct ggagaggttc atcaaaaccc attgcaatca
    33301 ccgggatctc atccacaaca agccctacgc cgtgaccaag gaactgaact gtccgctctc
    33361 caaagcccat gaaattggtg aggaattcct ggtcaagacc cgccatgatc gtggtgtaaa
    33421 tcgtcgaggg ggccacccct ggtttcagca gcgatgcgat ctcgtcctgc agctccacgc
    33481 agcggctatg gatctcaatt gcatactccg ggacaggtct cccgaacatg tagttcatcg
    33541 tcttgtcggt ctggtatccc tctacgccgc atgaattgtc gatgaataca aggtccccgg
    33601 atcgcagctt ccgggtatgg ctaccaagca ccggagcccc cggatatgct ccgtaacacc
    33661 cgccaggccc gtcgaagcag gtcgggtaga gggaactctc tccaaatcca atctgcccca
    33721 cctcgatctc ggtgttaaaa cgtccgaaac ggaccacccc ctggtgtccc cgctcaacca
    33781 tcagggagta tatagcggtc ccgaactccg cctcgctcat cccctgcctg agaaccttcg
    33841 gcgcttcctc ttcgagcaca atcctgtgaa tctccccagc cttctcaagg agtgcaagct
    33901 catacgggct cttgattgac ctgacacggg ctgcctgcaa atccattggc gaaacctcgg
    33961 agaagggaaa gtgtttcctg aaccgttctg caagcgcgag cggcacaacc tcggtctcca
    34021 ggtatatccg gttcggatgg cttacggtat cgagagccgc gtcgcgataa ctcctcattg
    34081 gcctgatatc agggaagttt gactcctcaa tcgcacggtc gtagctccgg cgcacccaga
    34141 agaccgcgtc tttgtccctg ccgacgagga gcacgccgtc ctggatggtc cctgtaaaat
    34201 agtacaggtt gatcttcccg aagatcgcca ccatctccca accctggctg tcatcgtcca
    34261 tccgggcaag gaaccggctc atgcggtcct gaagttccct ggcgggtacg tggtcatgca
    34321 tgcaacagag gaatgggacc atggtgttat ttacccctta ctttttttcg gcattcgcac
    34381 atcccggtca ggaactatta aatcccttcc ggggaagaaa tctctcctgt aggagtgatc
    34441 atgatccatg cttgacatat tcaaatgcga gaagtgcggc aagattgtca tggtcgtcag
    34501 cgatggcgac gggcagcttg tctgttgcgg gatgccgatg aagagacagg atgaaaacac
    34561 tgtcgacgcc agcaatgaga agcacgtgcc ggtggtcgaa ccccatggca aggagatcct
    34621 cgtcagggtc gggtcagtcc ctcacccgat ggagcctgcc caccacctcc agtggatcga
    34681 agtaatgtcg ggccccatgc tctacgtgaa ggggctgaaa ccgggcgaaa agccggaagc
    34741 cgtgttccct gtctccacga cagatgtcaa ggtaaggacg tactgcaacc tgcacggatt
    34801 gtggtcaaac aagcctcacc acaggtgaac gcctcccact cacattcttt taccagtgcg
    34861 cagtagcccg gggaccgcgc tcccataccg taatctggag agagttccat aattcctgtt
    34921 cagatgccga ccggtaaaga cgcggcccag atctgatggt aagttgacca attatgaaga
    34981 tggaacgacc cggaaccggt tctcatgctg acacacctcc actgctcgaa tttgcacatg
    35041 tcacggtgat gaaaggagag aagaaactcc tcgacaacct cacggtgtgc attcgggacg
    35101 gcgagcatgt cgcaatactc ggcccgaacg gatcagggaa atcatccttt atccggaccg
    35161 tcaacaggga gtattatccg gtcctcggaa cggatggtga agtgatattc cgggtctgcg
    35221 gcagggatgt ctgggatgtc tttgacctga gagggctctt tggtatagtc tccccggacc
    35281 tccagtatat ttatacgagg aatgtgacgg gaagagaggt gatcctctca ggattcttct
    35341 cgagcgtcgg cctcttcaac caccatatca ccccggagat ggaagagagg gccgaccaga
    35401 tcatccaatt cctcgaagtc gggcatctcg tgaaccgaag gatggatgcc atgtcatccg
    35461 gagaggcgcg ccgtttcctg atcggaaggg ctctagtaca taatccacgg gccctcatcc
    35521 ttgacgagcc gacgaacagc cttgacctcc acgccctcca cacgttccgg caggccctcc
    35581 ggaagatagc cgggtcgggt gtgggtatca tcatggccac acataacctt cacgacatca
    35641 tccccgacat tacccgggtg atcctgatgc gggatggggg gtttttcatg gatggaccaa
    35701 aagaggacgt gctgaccgat gccaatatcg gagacctgtt ccgggttcct ttcaggatca
    35761 gagaagaagg cggatggtat tatgcaagcg ggtattagat gagatgtgcc gggattcaag
    35821 ctgggaaaaa aggctttctg cgcccttaac agaattccct gacccttccc acgctgccat
    35881 cctccagccg gacctttatt ccatggggat gaaacgtcga gttggtaagg acctccttca
    35941 ccttcccctc cgtaagtcgc ccggttccct ggtcctgttt cttgactatc ctcaccatcg
    36001 ccccggggaa gatcccgctc cggttcctgc cgtccttcct cgactcttcc ggggctctgt
    36061 aatggaatga gggatctttt ctcatgagat gcgccaccgc gccagatttg gcgtcttgac
    36121 agttccctgg atcttccact tctggagagt aaataccatg ggtcgcggat ccctacctgc
    36181 tggtattatc actcaccaga aagattctct ctcggaagcg attcaacatg acgcacatgc
    36241 agttcacctt ctttgcactc ctctttgcgg tcgcactggc gggggtcgtc accgcagatg
    36301 tgaccatgca ggccggcgcc gctggctcag gcagcgtata tacgacagga aacatctacg
    36361 taagctctgc tccggcagga gcttctgcca tacttgacgg ggggcattca caactcttca
    36421 ccgccggaac cttcaggggc gtggagccgg gaacccacca ggtgctgatc acattgcagg
    36481 gcttccagcc ctatacgaca accgtaaaag tcagcgccgg tgccaccggg aatgtcattg
    36541 cgacactgcc accagtcaca agcccggggg gtatatcggt cagctcgacc ccgaggggag
    36601 caggggtata cgtagatgac atctacatgg gcaagacgaa ccaggtggtg ggaaaccttg
    36661 cagcaggccc gcatcgggtg gtcgtctctg aagcagggta cgtgacctgg gaagagaccg
    36721 tccaggtaag gtcaaaggag gtcatccccg tggcggtcac cctcgtggca gaggtgaacg
    36781 cggccagtgg ggatctcctt gtctcatcca cccccacggg tgcgtccgta tacatcgacg
    36841 ggaactatcg cggctcaacg ccggttgacg actcccttga catcaacgat ctctctcccg
    36901 gcacctacac gatctccgtg aagaaaccgg gataccagga gtatgcaaca accatcaccc
    36961 tccaggcggg gaggaaagtg caggtctttg ccccgctcac gcctgccgag cagaccgcag
    37021 cgagtgtaga gatcagctca acgcctgccg gggccgacgt ctttgtcaac aacctgtaca
    37081 tgggcatcac cccactctcc ttccatgacg tttctgccgg gacctacacc gttgatatcc
    37141 ggatggaggg gtatgtcccc ttctcttcca gcggtcaggt gattccaggc cagagcgtcc
    37201 acgtgattgc ggcgttgtca ccggttcaga cccccgctcc cacgacaaag gcgccgaccg
    37261 gggcattggc ggtagtcatg gcttttgtaa tgaccgggat tgtctgcctc ctggttgcaa
    37321 ggagatagag gaatcaacaa tttttttgcc cggattctcg ggctcagcaa cactaagcgc
    37381 ctcgcggagg gctgcaatct cgacacggtc cgactcgatg gcatagagtg aagtgccgta
    37441 gcatggcttc ctgaacggca tcaggatcct gcctgcctcc tcgtcgatcc cgaccagcat
    37501 tggcctctca atcaggattg tgacaccccg gaagacgtat ccaggtgtga taaggtagtg
    37561 ctccctgcat gcctttctca ccctgttgtc tgcacggcgg ggggtagcat ccttgaggat
    37621 aatgtctgac cagatccggt tgaaacaggg ttccggagac atgatgggaa agtaagcacc
    37681 ggggaggaaa agatttgcgg cgaagcccgg ccgggccgaa acgatatttt cgattcccgg
    37741 ggtatccatt cacatcacga tcgagagtgc gtccgtgatg aggggcagtc gggataagtt
    37801 atgtttaaat aacataatgt tgtatatttc tcatattagt tagaaatatt taacagagag
    37861 gggcaaaatg tttgcacaga aaccaaccgt cttcaagtcg cgtgcctgga tcaccgtgat
    37921 gttcatattc gggatagtgc tgattggaat cggcatgatg aatctggctt tcctggcagg
    37981 tgaagaatcg ccgggcctca tttccatgtt catcagcgcc ggttttgtcc tcacgatcct
    38041 ctcgggtttc cgtctctgga agggcgaaac gaattacatg caggacgaac ggacaaagag
    38101 gatcggtgca tacggactct cctggtcctg gttcctcaca ttcctgttcc tcttcgggat
    38161 cttctgggca gattacctcc acctctggtc tcctgatgcg cagacgctct ccgtgctcct
    38221 catccttttc atgggcatat cggccaaggg cttccaggca tacctcttcc ggaaaggaga
    38281 cgtggattga cgaacccggg gatgtttcca tgcagacaag aataaaagag tttcgcgcga
    38341 agagggccct gacacaggcc gaacttgccg acctcgtagg ggtccgcaga gagactatcg
    38401 tctttttgga ggcaggcaag tacaacccct cacttcgact cgcccaccag gtggcaaaag
    38461 agcttgggac cactatagac acgctcttta tcttcggcga tgagggtcac tacacggtga
    38521 ccgaggaata acctgccagg ggagttcccg tcatgccgat atgctccacc acacgaccac
    38581 cttattctgg tctccgccat gcccgcgggg ggatatggac ctcgaacctc gcccccttcc
    38641 cggcctttcc agtctcccgg atggtgatcc cggtgagcga gaggatgtcc cgggagattg
    38701 caagcccaat ccccgtgtgt ttcccatatc catagcggaa aatcatctcc ttctcgttgt
    38761 cgggaatgcc aaccccgtca tcctcgaaga ccaggattag ctcgccggcg ggcccttctt
    38821 tggtccatat ccggacagag ctcacatcct caccgtgccg catggcgttg tcgagaaggt
    38881 tcgagaaaac ccttcttgcg agagggtcgg cattgatctc cacccgctcg agagtcggca
    38941 tgacccctat accctcgtgg gggacatcct caatggcatg tgcgaccatg tgtccgagat
    39001 cctgccaggc cggctggttc gagccgaatt cctggtagtc ctgtgcgaac cggaggtggc
//
LOCUS       DAOQYR010000013        38951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_1208_length_38951_cov_8.833402, whole genome shotgun sequence.
ACCESSION   DAOQYR010000013 DAOQYR010000000
VERSION     DAOQYR010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 38951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38951
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1208_length_38951_cov_8.833402"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="PKM87_02955"
     CDS             complement(<1..1286)
                     /locus_tag="PKM87_02955"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNQ29057.1"
                     /translation="MLLFTILVFFCLLSAFITFGLGIYVYAKNSASPVNRLFLVVMLA
                     ATYWAMGEFLIWHAADYGNVWLWLKFSSFWPFVIAFTVHFVLVFTEHPLSRPGKSNFL
                     VIILYLPAAIISLFGVFSDTIYSVAYAEGLGYIYLPVQGSPVYAAEVIYVLAAMIWAV
                     YVAIDSWRSASRGRVRRQNRLVCAGLVTIIVCGALSGIILPAFGIFTPNLVFIGIVAF
                     TLIITYAVHTCGLFVLSPKTALPEILRTMPDGMVLLNQGGEVIAANEAALKLFAAEEQ
                     GIRGREISALLPGREYERIRNVILGEGSVSDVEAAIGKDYCISVSIAGALVKGPEGEP
                     AGSVLVIRDITARKESEKALRIANEKISLLTKLTRHDISNLVSALLAYLELMREDADS
                     RTHDAYLSSCIQIVERIASHLRFAQDYQEFGSNQPA"
     gene            complement(1426..1626)
                     /locus_tag="PKM87_02960"
     CDS             complement(1426..1626)
                     /locus_tag="PKM87_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNQ29058.1"
                     /translation="MEVYIDRIFCNGCGTCEEMCSMGVFQVNHRTHKAEAIRPHLCIG
                     CFKCREFCPTQAIAPRWIMRVS"
     gene            complement(1866..2294)
                     /locus_tag="PKM87_02965"
     CDS             complement(1866..2294)
                     /locus_tag="PKM87_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29059.1"
                     /translation="MDTRFCARYQGDGFPPSNRFCRTCKTEACSALWEKVIALSRSHD
                     GMPVGLPPTRAVMLPHDANGSMVKMRVNVTWTLPKEDFLHYVATGKAGMGRKGDRQNP
                     ESSPSMTRQEPYVQAIIEAIGGWGIPEILMVREVQKGVEM"
     gene            2374..3003
                     /locus_tag="PKM87_02970"
     CDS             2374..3003
                     /locus_tag="PKM87_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29060.1"
                     /translation="MPSNPSRILLLVLLSTGFAICSAQALLAPGNIQVSPPGDSMPVG
                     SIVNVSATIEIIPSGATTFAETHTLSLSTGLVDARWHVVVTVDGQQAAVIPKDGPQVF
                     VNGFLLSYPTTRDVSVSIAVDGRAPPDPAAGTVVVLEWAELNSQGLVVAGSDYQVTRQ
                     AETFPASPVVTTATQAPVLTTSPANIPFPVMGAAASLCLVFGWIGRRKG"
     gene            3445..4227
                     /gene="modA"
                     /locus_tag="PKM87_02975"
     CDS             3445..4227
                     /gene="modA"
                     /locus_tag="PKM87_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNQ29061.1"
                     /translation="MDRLHRVLSIVVFTALIAGIVFFTGCTTSPTTTEKPNLNLYVAA
                     GMKKPMDVIVDKYKAGSTGNVTPNYGPSGGLYTQITLGQPCDLYYSADWMYIEKLQNE
                     SRTSEVHKFLKDYIVMVVSKTGKEKGITSTADLTGDGVVIAVADPAAPVGAYAENALK
                     SLGLWDQLNAMGNIKTRPNTVNQLALMVQNDEVDVGFVYSSTANLYGLDVVEKFSHDQ
                     TGEIVFGAAVIKGGNEAAAKDFLAFSTQNAGEFIAYGWEPYA"
     gene            4220..5008
                     /locus_tag="PKM87_02980"
     CDS             4220..5008
                     /locus_tag="PKM87_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ29062.1"
                     /translation="MRKVSTWELLFVPLFFLLIVYPIAAIVGNVTIEGMERTFADPIF
                     WDAVKNTLITAFFAALLGVLMALGFGYFYLFHPGTIVYKIADFMNDFPIALPHTVAGL
                     ALLLAFGRKMFWFAGPTGLAFTLLAVALAMFFVSYPLAARTIAASADEIDMDLIAVAR
                     TLGDSPAHAYSRVVIPILGEAIFSAYVLSFARSLSEFAAVIMFGGNVPGMTQVLASYV
                     FSKVEEGEIGMAVTASAFCILLSLLLVLILRLRSKYYARNTGCA"
     gene            4983..5669
                     /locus_tag="PKM87_02985"
     CDS             4983..5669
                     /locus_tag="PKM87_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ29063.1"
                     /translation="MLEIQDVHKSFGDQVVLKGVNLEVSPGELQVLIGLNGSGKTTLL
                     KILTGLLTPDKGRVRIDGRDVTLFPPEERYIGYVPQHTALFNHLTVKENILYSLRHKR
                     GSEETFHQMVDLLALDPYLERKPRELSGGYRSRVALARALTSMPRAMLLDEPITEVDR
                     AKKEHILPLFRRVLVKMNIPVLYITHDPWEAEQIGTSFSIMKEGSVTRINSPAEAFAI
                     VREQALCDEE"
     gene            5871..6611
                     /locus_tag="PKM87_02990"
     CDS             5871..6611
                     /locus_tag="PKM87_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNQ29064.1"
                     /translation="MHPTGATFMEMTRYEHLSPSDQYQGLPAPPLEKPFPRDGNFIPL
                     PDPDTLQVPMLDLRDAIEERRSLRHYTRDPLSLEELSYLLWCTQGIVQVQDPYYTLRN
                     VPSAGGRHSFETYLLLNRVTGVKPGLYRYLSFSHRLLEMDTRLGIADRVMAACLGQAF
                     VRASAVTFLWACVVYRMAWRYAERAWRLVHIDAGHAGQNLYLAAGQVGCGACAIGAFD
                     DQQAADVLGIDGVNEFVIYCAAIGKKGG"
     gene            complement(6620..7414)
                     /gene="trpA"
                     /locus_tag="PKM87_02995"
     CDS             complement(6620..7414)
                     /gene="trpA"
                     /locus_tag="PKM87_02995"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNQ29065.1"
                     /translation="MTTRIVRAFSARTRPLLVACLVAGDPDYGESLAMMREVDRAGAD
                     IIEVVMPFSDPVADGPVMQQAIQRALSAGMNTDSLFSLIRDFRAESETPLVVLTYANP
                     VVQRGIGAFYRDAGGAGVDGVAVADVPLEEADSFCRAARKAGVDPILFVSQTTSMERL
                     KGITAKAGGFLYLVAALGVTGMRGEVDPATIGCIRKIKEMSSLPVVPGFGISSPGQVQ
                     AYSRAGADGIIVGSAIVREVGDRLGRPGEMRAAVGEMVRSLVAGMA"
     gene            complement(7411..8583)
                     /gene="trpB"
                     /locus_tag="PKM87_03000"
     CDS             complement(7411..8583)
                     /gene="trpB"
                     /locus_tag="PKM87_03000"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNQ29066.1"
                     /translation="MIESTRFGDYGGCFVPETLMAALEELEEGFRTIVPGASFQQEMH
                     YYLHEYAGRETPLTFCRNLSRDLGCRVYLKREDLLHSGAHKLNNALGQALLAKCMGKR
                     RIIAETGAGQHGVATAIAGAALGMKVEVYMGEEDIGRQALNVARMRMLGASVLPVESG
                     SRTLKDAINEALRDWVANVRDTHYLIGSVVGPHPFPTLVRDFQTVIGRETRVQVLEKE
                     GRLPDAIVACVGGGSNAIGIFTPFLDDGTLLFGAEAGGEGLEGRHGATLCRGRPGVLH
                     GTYTYLIQDAFGQILESHSVSAGLDYPGVGPEHSFLRDKGRVTYRAVTDDEALDSFLY
                     LSRTEGIIPALESAHAVSLARRIAQDLEKDDVLVVNLSGRGDKDVAQVAALGGDSL"
     gene            complement(8576..9157)
                     /locus_tag="PKM87_03005"
     CDS             complement(8576..9157)
                     /locus_tag="PKM87_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNQ29067.1"
                     /translation="MRVKICGITSPGDAVMADQAGADAIGVVMYSDSPRSVDIETAEE
                     IFSAAGPYIARVCVSHTRSLGDIEAMLDLNPDVIQISSSLALPHPFPVRLIRVVAGGR
                     ALPIDADAVLVDGSAGKGMVYDEEYASRVVRESRVPVILAGGLNPGNVALAVRRVRPY
                     GVDVATGVEDAPGVKNERKVREFVRIAKGAVYD"
     gene            complement(9148..9909)
                     /locus_tag="PKM87_03010"
     CDS             complement(9148..9909)
                     /locus_tag="PKM87_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HNQ29068.1"
                     /translation="MILDEIISSTGVRVAGLPEQYPVGVTGGKQKMSLVGVMGERNGR
                     NAIISEIKYASPSRGVIRESGDPVELARMMVSAGCAALSVITEPSFFHGHPGLIPLIR
                     PHVEVPILRKDFIIDERQLAETRALGADAVLLITSVLRDRLPEFVDMAAGYSLEPLVE
                     VRTSGEVANAVDSGARLVGINNRNLADFSIDLSTTRRLAPLIRDAGCRVVSESGFVWP
                     YDVRSLRKVVDGFLIGSSVMSAKDPKKRLGGFVSA"
     gene            complement(9906..10922)
                     /gene="trpD"
                     /locus_tag="PKM87_03015"
     CDS             complement(9906..10922)
                     /gene="trpD"
                     /locus_tag="PKM87_03015"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNQ29069.1"
                     /translation="MMQTVLQVLSEGRLLSDPEAREAMEYIASGRATDAQMGAFLAAL
                     RIRGENAEEIATFGRVLQHYAVTIRPRVAGTLVDTCGTGGDGCRTFNISTAAAIVAAG
                     AGVVIVKHGNRSVSSSCGSADVLEALGVKIDIQPERVCRIVEETGIGFLFAPAFHPAF
                     RNAARVRKDLGFHTVFNMLGPLLNPAGAQSRLMGVYSPAFLRTLAESLQLMGAERAMV
                     VHGSGLDEITTTGPTQVAELAGGTIREYTFTPGEFGIPVSSGNALRGGTPAENAAVVL
                     RILEGGEGPGRDVVVMNAGAAIYLAGKADDLAEGITLAKESIRTGAALGKLEALVTAS
                     GRAS"
     gene            complement(10919..11494)
                     /locus_tag="PKM87_03020"
     CDS             complement(10919..11494)
                     /locus_tag="PKM87_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNQ29070.1"
                     /translation="MRVVIVDCFDSFTYNLYQMVGMLGAVPVPVTCDRTLSAVRKESP
                     DRIILSPGPGTPEDSGVCPEVVSEFEGRVPILGVCLGHQTIIHCHGGAIRRMDAPVHG
                     KTSRIVHDGQGLFAGLPNPFTATRYHSLAAPADRVPEEFETVALSEEDRCVMGVTHRE
                     YPLYGLQFHPESIMTPEGRVIMENFLFRGGA"
     gene            complement(11491..13059)
                     /locus_tag="PKM87_03025"
     CDS             complement(11491..13059)
                     /locus_tag="PKM87_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HNQ29071.1"
                     /translation="MYINKHDKRERTTPGDAAGMGIAAFAAAYSDRPEGLIPAGRNAG
                     PIVVLPVTRRIPLPAADPYALFRGLGAGTGCILESMEGVPRRAVRSIVGLEPPLVFTL
                     DEKVTCKGDQRLSNTDSFPQGSDPVEQLRRLCNRFRCEGGGVTGFAGGLVGYCAYDMV
                     TDLSAGLVPAGKEGPLARFMLCTRGIVYDHVHGSCLLFDDPLLIEGEDTGEAYDRANE
                     RMDLLEEAVMAVPGADPGEQGPGGGMELPLRTTMDREEFMTAVRKAKEHIRAGDIFQV
                     VLSRRLSCPYTGDPVLLYGAIRTINPSPYLYCIRFGDETVIGSSPEMLVKVEGKAVQT
                     VPIAGTRPRGSDLREDDQLAADLLADPKERAEHLMLVDLARNDIGRVSAFGSVQVPEF
                     MEVERFSHVQHLVSRVCGTLRDGCDRFDALAACFPAGTVSGAPKIRAMQIIADLEAHP
                     RGLYAGAVGYIGFNDALEFAIAIRTLVAREGVVEFSTGAGIVADSVPEREFEETGYKA
                     GAMEKAIRQAGVGR"
     gene            13099..13227
                     /locus_tag="PKM87_03030"
     CDS             13099..13227
                     /locus_tag="PKM87_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29072.1"
                     /translation="MYRYPLDEAGLVKTLATVRITTFLMAALPETPHGNVYPFGIQ"
     gene            13254..13616
                     /locus_tag="PKM87_03035"
     CDS             13254..13616
                     /locus_tag="PKM87_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNQ29073.1"
                     /translation="MVTIVHFDIPADIPDRARNFYAALFGWKFTSPPGYPDFSLIETT
                     GLEGHPGVGGGLGRRGEPGQDITLYFGVESVERYLALVIREGGKVVMPYTPVPGYGAL
                     AICTDTEGNRFGLWENTA"
     gene            13670..14161
                     /locus_tag="PKM87_03040"
     CDS             13670..14161
                     /locus_tag="PKM87_03040"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNQ29074.1"
                     /translation="MAAQRPDPMTGQPAPSFCLPDSKGNEVCLPQFRGKWVVLYFYPR
                     DNTPGCTLEALSFHNALESFAGMGAQILGVSADTPESHQRFADRHNLSILLLSDTGHQ
                     VIEAYHSWKPKKIFGKEVLGTQRDSFLIDPEGRVVEVWRKVSPKGHADEVREALVGRL
                     GRS"
     gene            complement(14195..14869)
                     /locus_tag="PKM87_03045"
     CDS             complement(14195..14869)
                     /locus_tag="PKM87_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNQ29075.1"
                     /translation="MENVREAFDAIASGYDRQRRFVIPQMEEFYEAAVWAAAWPGESP
                     SILDIGAGTGLLSAFLLEKYPRATLTLLDISEKMLEVATERFAGNPRVRFVAADYSRE
                     ELPGRYDLVCSALSIHHLEHGDKRRLFRRIFEALNSGGLFVNADQAEAQSEWLSRRNI
                     EYWDAFVSASPFPRDELRAAMTRRDTLDRNATLLDQISWLSEAGFSGVDVVYKNRTFC
                     VFVGRR"
     gene            15025..15840
                     /locus_tag="PKM87_03050"
     CDS             15025..15840
                     /locus_tag="PKM87_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNQ29076.1"
                     /translation="MTIGDPAGYVHGHSEREAVRLSDQACTLEELLHHDTFYPPQSLV
                     LEAGCGVGAQTGILAARSSEARIVSLDLSLSSLSCARASAAAGGWEEVTFARGDLHSF
                     PFRNGSFDHVFICFVLEHLQAPLIALQSLREVLKPGGTLTVIEGDHGSAFFHPESRHA
                     SDAILALESLQRYSGGNPRIGRELYPLLTSSGFHDVHVSPRQVYVDDSKPGLVEGFTR
                     QTFTAMVEGVREKVITSGMMPEEDFDRGVRDLYRTSEGGGTFCYTFFKAVAVR"
     gene            complement(15989..17359)
                     /locus_tag="PKM87_03055"
     CDS             complement(15989..17359)
                     /locus_tag="PKM87_03055"
                     /inference="COORDINATES: protein motif:HMM:NF025774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ29077.1"
                     /translation="MNTPRIKEQGGLAPGDHAAILYQQSGEIVEVTGSILDQCRRNRW
                     HLLYLSRLPDENPLIIGKTGRVAEENNVTVLSHQDEEFHRHFATARNAKAFITERVRM
                     ARSAGSPALCIMREVPPISARRSSQHLVGDMAGLDHLFKEKSLLLLCAYRMDLFPAPV
                     LQDVLRTHPLILLGGRVVENLFYIPAMDALRYNLPSLELQHWLDTLRKLSSARAELAE
                     SEGRLQDLLENANDLIQSVGPDGRFLYVNQAWRKTLEYSPEEIAGLNIFDIIDPSSME
                     HCRITFGRVMTGEDVGRIEATFRSKTGRKVYVEGNVNCHMENGIPQYTRAIFRDVTDL
                     KREEMARREREQVLDAVLNLTPHATVITTPDGTMLYANKPARSISPKTSTGGVHGKPL
                     WSFLSPGGEEEVRRSMADLTMVGEKTTSCRVIGCDGRDWEIEWSRARFGSKDASYLMA
                     VLRRIE"
     gene            complement(17484..18638)
                     /locus_tag="PKM87_03060"
     CDS             complement(17484..18638)
                     /locus_tag="PKM87_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-NAD kinase family protein"
                     /protein_id="HNQ29078.1"
                     /translation="MCTIGFLVNPFAGMGGAVGLKGTDGMVEAALARGAVPRAPGRAA
                     ETILQVADIGAHFITCSGVMGEDILAGAGIPGYEVVYRPASETTGAADTMAACRAFLD
                     RDADLILFCGGDGTARDVYSVIGDLVPMLGIPAGVKMYSAVFAIHPRAAAGIVREGCT
                     PGGKFLLRDAEVVDVDEEAYREGSLRTRLFGIAKSPYRPGLVQGTKQVFEDSDEERAK
                     KDIARFMREVIEGTPGILYILGPGSTTAAIGEEIGLKKTLLGFDAVKGGRLIATDLNE
                     EGLLGLLEEGTSARLVISIIGAQGSVLGRGTQQVSPAVLRRIGRENVIIVATPHKLAE
                     TPMVFVDSGDRDMDLAFGDHLSVVSGYRIAQRKRIGRVEGPERTSQVGGE"
     gene            complement(18732..20096)
                     /locus_tag="PKM87_03065"
     CDS             complement(18732..20096)
                     /locus_tag="PKM87_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNQ29079.1"
                     /translation="MHTKIVLDEDHMPKKWYNILPDLPTPLDPPLHPATGKPLVPEDL
                     TPIFPMELILQEMSQKRTIDIPSDVQDVLRLWRPSPLYRAHRLEKYLKTPAHIYYKYE
                     GVSPPGSHKPNTAVPQAYYNMKAGTERIATETGAGQWGSALAFATQIFGLECTIYMVR
                     SSYMQKPYRKSMMQVWGAECIPSPSTKTKAGTAVLQKDPETTGSLGIAISEAVEDAAT
                     HGNTNYSLGSVLNHVCLHQTVVGLECMEQLASVEDYPDVVIGCVGGGSNFAGISFPFA
                     GEKLKGKHKETEIIAVEPASCPTLTKGLFAYDYGDVAGLTPLLKMFTLGHDFVPPSIH
                     AGGLRYHGDNPTISRLVYDGVMKAVSYHQSEVFDAAQTFARTEGIIVAPETSHAVRAT
                     IDEALKCKKSGEAKTLLFNCSGHGNFDMSAYDAFYANQLVDYEYPEQLIRESLARLPK
                     IPGA"
     gene            complement(20203..22278)
                     /locus_tag="PKM87_03070"
     CDS             complement(20203..22278)
                     /locus_tag="PKM87_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HNQ29080.1"
                     /translation="MESQIWQPLPGVEGVSIYPFLRKVDIISSNSYLVAAPRLIVLVD
                     PGGLPGQADTLLGEIRKLITEKPRPVLICLTHVHLDHCHQLLHHPGFRTLPHMLVAVQ
                     EAGARALESRDRAATIADLIRQEVGTITIDIRLLPSGNGGEHRDEILNIAGSSVITCL
                     DTLPLGGGLTISRQTLPLEGDTMIEFYPAPGHSPDSICIRMGETLFLGDLLFSTAPGV
                     AGLKGWDLKALLFTLDQVIALLQKGGITVCLPGHGRPLDAATTTRTLQAMKKEASRLE
                     GIEIVNPEWVKDTAAYAGELMGEVDRLFTIIAGRLVYVAHVLDELEESGEAERFRGLI
                     NSDLVDEILSDFNRFSLDFHSGQALEIHLALKANQISQKLEKVYRGEMLGSVLDLSLV
                     SRVGRLLEDYTVTFRGFRPRIDLAATDLSAAIGEVVAAISRPPYEEEGIIEAQTEEEF
                     ARALAVRIAYVNPFEHLKIEYSPGGNLPPAMMDRERFGDMFLYILEKVSVAGAQSLHI
                     ATGCTKDQVWVKLQFPASSAFTEENVRRFAARSCSLSNGSCSVSSTRGSTTVFIEYPA
                     AGKSSDTAIGEVPCRMEEPVPAGHRIIAPCGRIDAASAPMLEGELSKAIDEGHRRIVV
                     NLSSVDYMSSAGLRVLLAAMKRLKKAEGNLVLCAMKPFVQEVFDLTGFSRIFTICDSE
                     EEATHLLGP"
     gene            22392..>22885
                     /locus_tag="PKM87_03075"
     CDS             22392..>22885
                     /locus_tag="PKM87_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29081.1"
                     /translation="MQEPDDRQPIRFSAPVNDQWNYLLSTIWNQMMHLVVILDGRLDE
                     ERLRHAAELVMEAEPVLACRFVEAAVPYWEWIPSLRSESVFSIAGQGSICETSLKGAL
                     ARKYDPAAGPQAHFTLLRGDRDSLVISVNHAVCDARGILHISSLFARAYRALESNSGF
                     SLSCA"
     assembly_gap    22886..22985
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22986..23327
                     /locus_tag="PKM87_03080"
     CDS             <22986..23327
                     /locus_tag="PKM87_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29082.1"
                     /translation="IRLEETFSSGFHSVREHLLDAKRRSLIEGYPKNPFFSNIGILPI
                     PCADFKDIPTRQAFIMPPVEYSPGFGITASTFRDTLTLSCGFCEGPLKGGTVERVLFS
                     MEEFISSLTRE"
     gene            23324..23515
                     /locus_tag="PKM87_03085"
     CDS             23324..23515
                     /locus_tag="PKM87_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29083.1"
                     /translation="MRTAQSGRPGGIRSVTGTMVYIHRMTGTTPVTLRVKPARIMSLG
                     LLCPVPYVMVLAAAAPRTS"
     gene            complement(23635..24132)
                     /locus_tag="PKM87_03090"
     CDS             complement(23635..24132)
                     /locus_tag="PKM87_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ29084.1"
                     /translation="MKIGIIGDTHDHLPNIGRAVEALIQEEVDMVLHTGDYIAPFVIP
                     LLGRIPVRVVGVFGNNDGDRELLATRSREQQNIELSGFFADIRVDGERVALMHGHDRA
                     LLAECIGSAKYDIVVSGHTHQCSIYREERTLGINPGEICGYLTGNPTYAIYETGERVA
                     RLLPL"
     gene            24210..24575
                     /locus_tag="PKM87_03095"
     CDS             24210..24575
                     /locus_tag="PKM87_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29085.1"
                     /translation="MTGREDEREIVCGLERAIVGNDRIARRRLLDRLVVLLFTTDTAA
                     PALVSHGGIRVLLTAARDRDEKIRLHAVHALSRLADSGFASELRDAGARTSIAPLMED
                     PYLPLRAMAEKLLEKIDGS"
     gene            24726..26156
                     /locus_tag="PKM87_03100"
     CDS             24726..26156
                     /locus_tag="PKM87_03100"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNQ29086.1"
                     /translation="MKMRIGGVEVAGADERWMPVVNPATGEEIDRVPEGTLYEVDDAV
                     KAAGDAFSSWAGRAPRERGLLLFRGAALVRERHENLARLLTMEQGKPLKDAVDEVRGF
                     ANVLEYYAALSGSLAGEYIHLGKAGDAVVAREPFGVCGAIVPWNMPALLMAWKVAPAL
                     VAGNTMVFKPASTTPLTSIRLAHILDEAGLPPGVLNVVTGRGEVVGEALVRHPGVQKI
                     SFTGDAETGLQIQELASGSLKPVTLELGGSDPMIVWKDADIEKAVAGAVRGRFYNAGQ
                     TCTAVKRLFVHQSIGEEFVRRLMEAVRTLKVGNGLSPGVDMGPLSGAPQRERIGTLME
                     TLKNRGQGKVLTGGMPLKGPGYDAGYFYAPTLVTDVHPESALLTKEVFGPVLPVICIP
                     DLDTAIMHANNTRFGLGASVWTHDLSVAREVFSRVRAGVVWVNRHLTLPPDIPFGGVK
                     TSGIGSENGTQALSSYTRLRSLYIGW"
     gene            26388..26756
                     /locus_tag="PKM87_03105"
     CDS             26388..26756
                     /locus_tag="PKM87_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HNQ29087.1"
                     /translation="MLKITADELDRLLSPAHVVGQAVDLGDKAVIPVAEFGFGMGAGG
                     GKGKDGEGEGAGCGGKIAPVALVLVYKDVKGPDGIQVLSLHRDNPVAQIINALSESLA
                     PQVIKAIRAMSESQEKKQEG"
     gene            26772..27437
                     /locus_tag="PKM87_03110"
     CDS             26772..27437
                     /locus_tag="PKM87_03110"
                     /inference="COORDINATES: protein motif:HMM:NF022615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HNQ29088.1"
                     /translation="MDLHPVILVAAIVLALVVLELCLYAIPLRLRVRMDYANGAGMAF
                     ISACWLCLGVRSTLAPEGRTFHLLLGDLPLPLPAFLGSYAPGGRRPGDMALQEPEDLP
                     ASSYIQSAVPYVPYLISFARAAVRSLSFRRLDCRAVVGLSGPAETGMLYGYFWAAKPL
                     LCSSGRINLEMVPDFTREYLEGHLELDVNISYPFNLLARLIRLFTLLQENKTQSRKSG
                     VPA"
     gene            27434..27700
                     /locus_tag="PKM87_03115"
     CDS             27434..27700
                     /locus_tag="PKM87_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29089.1"
                     /translation="MMMSRELRIGPALKTGDRIVLAIFRQTKMTWPGGCTGNCTPVAL
                     VIVEGEECTVWQLGGDAAMEEIKDTLAGIAESLTLDAPITRSCL"
     gene            complement(27780..28949)
                     /locus_tag="PKM87_03120"
     CDS             complement(27780..28949)
                     /locus_tag="PKM87_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ29090.1"
                     /translation="MQRSLFFDFAVRNVRLHWFRSLLAVIGIIIGVVAIASMGILGNS
                     LVLAISDSLSTVGDSIIVTPHAGGGMGPSMGGSSELITDRKVEQIRRAVAPDLTIPVY
                     SGTARMRIGTEDLVGVVYALKPDDVPILLELEEGAFLRGSSGAMAGAKFAADNNLKVG
                     SRITIRDKGSLRVVGILAERGMGFDINPDYGIVVEDKWYQQAYDQRDYDMVIVKVKDL
                     TRIEQTKEAIEKQLNRRETEVDVIDTKAILETILTTFGQISTFTTAIGGISLIVAGVS
                     ILNIMMMSVTERIKEIGVLRSIGTMRKEVRSMFLFEALILGLIGSILGGVLSIFGGYA
                     VSIVMLQTTEYLFVPSSLVYIFYGMAFGVGTSLLSGLYPAWKASNLNPIEALRHE"
     gene            complement(29026..30228)
                     /locus_tag="PKM87_03125"
     CDS             complement(29026..30228)
                     /locus_tag="PKM87_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ29091.1"
                     /translation="MAGILISLAMRNLQRHWVRSLLAGIGIMIGVFAIASLGLLGNSL
                     VILFSGMITDVGDTLVITPHLAAASGDPFDPRSAVGAGIAERDLARITKAAGPNQVIP
                     VVEAGERLEVRDRGGYAILLALKSEHLPLLLSVREGVFPRETGVGVLVGALLAEEYDI
                     APGHIINVGGEPARVVGVLEERGMAIDINPDYAVVVTHGWFADRHPDPGYTRVLVKVG
                     DIGSMAAVKEDIDFQVNRQRERFDILDSREILDLFYSTYDAITLFLLGIGGVALLVSG
                     VSILNVMIISVTERTKEIGILRSIGTLRGQVMRMFLYEALVIGSIGSILGGVLSIAGG
                     YYLSVVVADTLTSSFSTVPDVRVFDLQGIGYIVLGVFFGILVSCLSGLYPAVRAVRIR
                     PIEALRYE"
     gene            complement(30236..31435)
                     /locus_tag="PKM87_03130"
     CDS             complement(30236..31435)
                     /locus_tag="PKM87_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ29092.1"
                     /translation="MIFLDLAKRNLKLHWIRSFLAILGIIIGVLAIASMGILGHSISL
                     LVANLISDVGDTVIVTPHVAAGQGFIGDPRVSVMARIPESDVEAIKKVVGSGRVIPVL
                     RSAEEMTYGDDSGYVTLLGLPAGEIPGLLTVEKGLYLKENAPGCLVGYYLAREFGISP
                     GSRVGIGNQTQRVSGILKERGYAADINPDYAIVVTDQWFFETFGDEKEYDQVIVKASD
                     ISQIDTLKKEIDQRLNRRDKVVDIYDSRDLLRQYDQIYNQIMVFLLGIGAISLLVAAV
                     SILNVMIISVTERIREVGILRSIGASRKDILMMFLYEAMMLGIIGSTIGGILSVVGGY
                     FISKVAIGVMTAGTTFGESASVFGPGTFVFIAGGVLFGVIVSTLSGLYPAWQASRMTP
                     IEALRHE"
     gene            complement(31432..32121)
                     /locus_tag="PKM87_03135"
     CDS             complement(31432..32121)
                     /locus_tag="PKM87_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ29093.1"
                     /translation="MISSPDGEKVIQFRDVTKIYPLPGGDVVALDHVSLEIRQGDFIA
                     IMGPSGSGKSTLLNLMGCLDTPTAGQLFIKGRDVGTMSDDELTKLRRDHIGFIFQQFN
                     LIPLLSALENVQYPLILKTGERTCTGRCVEVLRSMDLDEQFHSHKPSELSGGQQQRVA
                     IARALVNDPEILLADEPTGNLDTKTGSAIMSLLKELNEKGGKTIIMVTHDPNIAGFAH
                     RTIRIVDGKIT"
     gene            complement(32118..33254)
                     /locus_tag="PKM87_03140"
     CDS             complement(32118..33254)
                     /locus_tag="PKM87_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29094.1"
                     /translation="MNRAELLLFFIAVMVVFLPVSAVEQQTAAAQVMVTNVTLDPQVF
                     MVDDTGTVTVEVINNGAQSVAISRVTMYDEKIRIESRAYDTTMYLGAGNRMTFTFTVT
                     TTVPDGIYYPVFSMDFRDAGYLRYPVKLQVQDKPLKVSILSKPDVFTSGKKEQVEVLI
                     GNPRDNGVSGVIVYPMGEGIEASPSSIFIGMIAPDQSQKVTFSITPHQTTILEIHVDY
                     MNGINPHAEVIQFPIGIGKSKTRADPILSNIQVEKSGKTYSITGDVNNAGLEVANAVV
                     ISTGDSVVPVDPYRKYVVGTLQPDDFSAFEVTFTAEGTDKVPVEVSYKDNDGNLYTTM
                     TIIDIPVTKPGAQETEGMPLMMIAVILVCAAVIGGIVWFSWRKR"
     gene            complement(33256..33945)
                     /locus_tag="PKM87_03145"
     CDS             complement(33256..33945)
                     /locus_tag="PKM87_03145"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNQ29095.1"
                     /translation="MIHLLDLLVHPERFFSTLEGKEPDLKIPAAIALAGAVVAAVAGY
                     AQSSLFADMFAEMGAGAGMGAFMGIMGAAGAFLGFLIIWWLVMAGVFHVISMAFKGTG
                     KFTRTLESTGYGLLPTVIGSIISTLILFSYLPRIAVPVVRDLQDPVAIQKAMQELMLD
                     PAMKEFTQVSIAISILFLIWSANIWIFGVRQARGITLKHACITVIIPVAVYILYMVYM
                     LVSGIPLPGGA"
     gene            complement(34312..35244)
                     /locus_tag="PKM87_03150"
     CDS             complement(34312..35244)
                     /locus_tag="PKM87_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNQ29096.1"
                     /translation="MITIYRGGTDGLERVNKYESGTWVNVVNPTQKEIEELVSWFNIP
                     SDFLTDPLDVDERARIEREEGCILILLRSPRRELPDADIPFTTLPIGIIFTQNVLITV
                     SLNESDVIQEFFTGKVKHFSTDQQTRFVLLLFLRSTLVFMRYLKEINKMTTTVERSLY
                     KALKNEQLIRLLMLEKSLVFFVTSLRSNALMMEKFNTTSCMKMSEEDRDLFEDLIIEN
                     KQAIEMANIYSSILSDMMDAFASVISNNLNVVLKLLTTVTIILMIPTLVASIYGMNVE
                     LPYQHTPYAFLVTVAISLALSAVGIIIFWRKEFF"
     gene            complement(35440..36249)
                     /gene="budA"
                     /locus_tag="PKM87_03155"
     CDS             complement(35440..36249)
                     /gene="budA"
                     /locus_tag="PKM87_03155"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HNQ29097.1"
                     /translation="MQDRMVVAVCAVCVAAGFATGFWLAAMPENEIDQEVIYQVSTID
                     ALLQGSCEGTMTFGEVKKHGDFGIATFDSLDGEMIALGGDFYQIKADGQVVAVTDDMT
                     TPFGSVTFFSQDCTFPIRYAANFSDFSRQAEAFLPSRNHMYAIQIRGEFPYVKARSIP
                     RQETPNARLVDAAANQSIFEFHETRGTVVGFWTPDLVGGLNVPGFHLHYITEDRSAGG
                     HILDFVLENATVEMDVTSRFTVVLPTEGSFTEVDLSGDLSHELAVAEKGNA"
     gene            complement(36373..36822)
                     /locus_tag="PKM87_03160"
     CDS             complement(36373..36822)
                     /locus_tag="PKM87_03160"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNQ29098.1"
                     /translation="MKDKMHHRWMDCAVAEARAGLSEGGIPIGSVLVRDGQILGRGHN
                     RRVQEGNPILHAEIDCLQNAGRVGSFRNCVLYSTLMPCYLCAGAAVQFGIPVVVAGES
                     ENFPGARDLLEAKGVQVIDLHREDCISMMKEFITRCPELWNEDIGEL"
     gene            complement(36991..37410)
                     /locus_tag="PKM87_03165"
     CDS             complement(36991..37410)
                     /locus_tag="PKM87_03165"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNQ29099.1"
                     /translation="MTMRILFVCSRNAGRSQMAEGIARALLPTTSQVFSAGERPSVLN
                     GTAVRVMAEKGVDISRQSSKGLSSLPFREYDVLVVLGEWEDPSRLPPSRRVIREIFPD
                     PAAFSGADGDVLEGFRALRDCLWDRIFAWEREGQFRA"
     gene            complement(37442..38575)
                     /locus_tag="PKM87_03170"
     CDS             complement(37442..38575)
                     /locus_tag="PKM87_03170"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNQ29100.1"
                     /translation="MRELSRKVAGIAASATIEISNLARQMEKKGIPVISLSIGEPDFD
                     TPQHIKDACMEALKSGQTHYAPSNGIPELLNAISEKINRENGFSCIPEQVLVTCGAKD
                     AIYDAMEAVLNPGDEVIILDPSWVSYEPCVQVAGGRPVHHPLDTKAFQVSESVLDRVT
                     PRTKMVVVNSPSNPSGAVLDDASMKLISDMCTDYDLFALSDEIYEKLVYGKVPRSLAS
                     IGDMAERTITINGFSKAYAMTGWRLGYAVAPLPIIRQMSKIQQHSVSHPTTFAMYGGV
                     AALSCDQSCVEVMRKEFNRRRDYLVQELLLMGFEVAPADGAFYAYVKVEGDDMEIARR
                     WLEEVHVAVTPGSAFHTPGWERISYATSMENLREAVLRIRRCR"
     gene            complement(38572..>38951)
                     /gene="ribH"
                     /locus_tag="PKM87_03175"
     CDS             complement(38572..>38951)
                     /gene="ribH"
                     /locus_tag="PKM87_03175"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNQ29101.1"
                     /translation="EFNRDITYMMEVEGREHAAFLGAEVTECTYVPGAYDMPLAIKRM
                     LKRGKVDAVVTIGCVIEGATQHDEIVVQHAARKIIDLSLEFDKPVSLGISGPGMTRLE
                     ANERIDYARRAVESAVKMVQRSK"
BASE COUNT         7930 a        11804 c        10791 g         8326 t
ORIGIN      
        1 caggccggct ggttcgagcc gaattcctgg tagtcctgtg cgaaccggag gtggcttgca
       61 atcctctcca cgatctggat acacgatgag aggtacgcat catgcgtccg gctgtcggca
      121 tcctcgcgca tcagctcaag gtacgccagg agagcggata cgaggttgct gatatcatgg
      181 cgcgtcaatt tggtgagaag agatattttt tcgttggcta tcctgagcgc cttctcggat
      241 tccttgcgtg cagtgatatc cctgatgaca agcacgctgc cggcaggttc tccttcggga
      301 cccttcacga gtgctcccgc gatgctgact gaaatgcagt aatctttgcc gattgcagcc
      361 tccacgtcgg ataccgaccc ttcaccaagt atcacattgc ggatgcgttc atactctctc
      421 ccggggagga gggccgatat ctccctccca cgtataccct gttcctccgc tgcgaataat
      481 ttcaaggctg cctcatttgc agcgatcact tctcctccct gattaaggag gaccatccca
      541 tccggcatcg tcctgaggat ctcaggtaag gcggtcttgg ggctcagtac aaagaggccg
      601 caggtatgga ccgcatacgt gatgatcagg gtgaaggcaa cgattcctat gaagaccagg
      661 ttcggggtga atatgccgaa tgccgggagg attatgccgg agagggcacc gcacacgatg
      721 atggtgacaa ggccggcgca taccaggcgg ttctgccttc gaacccgccc ccttgatgcg
      781 gagcgccatg agtcgattgc cacgtacacc gcccatatca tcgctgcaag gacatagatc
      841 acttcggccg cgtaaaccgg gctcccctgc accgggaggt aaatatatcc caagccttcc
      901 gcgtaagcca cggaatagat agtgtctgaa aagaccccga agagggagat tatcgcggcc
      961 gggagataca gtatgatgac gagaaagttc gacttccctg gtcgggagag gggatgttct
     1021 gtaaaaacaa ggacgaaatg gacggtgaat gcgatgacaa acggccagaa cgaactgaat
     1081 ttaagccata gccatacatt cccgtaatct gctgcatgcc atatgaggaa ctctcccatg
     1141 gcccagtacg tggcggcaag catcacgacg aggaagaggc ggttgacagg agaagcagaa
     1201 ttcttggcat atacatagat accgagcccg aacgtgatga atgccgagag gaggcagaaa
     1261 aatacgagga ttgtgaagag gagcatggat atcaattgcc cgggaccagt cagcagcagg
     1321 gtgcggggcc cacccttttt tttatccttt ccagttacag tatctcctgc tgattctgtt
     1381 tccggaggag aaaacgcttg tgcatgccat cgccttttcc gctgttcacg agaccctcat
     1441 gatccagcgg ggggcgatcg cctgggtcgg gcagaattcc cggcacttga aacaaccgat
     1501 gcagaggtga ggccggatcg cttcagcctt gtgagtgcga tggttcacct ggaacacccc
     1561 catcgagcac atctcctcgc aggtcccgca cccgttgcag aaaatccggt cgatatatac
     1621 ctccatcagg agggtggggg agggtgttat ctccataatt ctatgccaga agtatccttc
     1681 gtgaacgcga tgataatcgt tgtggatgaa acgatatacc cattccccgg ttcctggaaa
     1741 caaccggcat ggtgttcatc aacacagttt cattcaacgg atgaaaatgc cattgtttta
     1801 aagggaccca tttcaacggg attaagaaga atgtcgaccg gtaactacca ctcaaaaaga
     1861 cactcttaca tttcaacccc tttctgaact tcccgaacca tcaaaatttc cggaatcccc
     1921 caacccccaa tcgcctcgat gattgcctgc acatacggct cctgcctcgt catgctgggg
     1981 gaggactcag ggttctgtcg gtctcccttc ctccccatcc ctgcctttcc ggtggcaaca
     2041 taatgaagaa aatcctcctt cgggagggtc cacgtgacgt tgacccgcat cttgaccatt
     2101 gacccgtttg catcgtgcgg gagcatcact gccctggtgg gagggagccc aacaggcatc
     2161 ccgtcatgcg atcgggagag ggcgatgact ttctcccaca gagcactgca cgcttcagtc
     2221 ttgcaggtcc ggcagaagcg gttcgatggc gggaacccgt ctccctggta ccgggcgcag
     2281 aacctcgtgt ccatcgcagt gctatggtac gtggcgggag taaaaaaaat gcgggggaag
     2341 gtatatcatc cacttacgcc actctcacaa tttatgccgt ccaatccttc ccgaatcctg
     2401 ctcctggtat tgctctcaac gggtttcgcc atctgttccg cccaggcgct ccttgccccg
     2461 ggcaatatcc aggtatcccc tcctggtgac tccatgcccg tgggatccat cgtgaacgtg
     2521 tctgccacca tcgagatcat tccatccggt gcgaccacct tcgccgagac ccatacgctt
     2581 tccctctcga cgggtctcgt ggacgcccgc tggcatgttg tcgtgacggt ggacgggcag
     2641 caggccgccg tcattccaaa agacggcccc caggtattcg tcaacgggtt cctgctctcc
     2701 tacccaacta cccgtgacgt gagcgtcagc atcgcggttg acgggagagc gcctcccgat
     2761 cccgccgcgg ggacggtcgt ggtgctcgaa tgggcggagc tgaactcaca gggccttgtc
     2821 gttgccggat cagactacca ggtcaccagg caggcggaaa ccttccctgc atctcctgtg
     2881 gtcacaaccg ccacgcaggc cccggttctg accacgtcgc ctgcgaatat cccgttcccg
     2941 gtgatgggtg ccgcagcttc actctgcctt gtctttggat ggataggaag gaggaaaggg
     3001 taagacgggg tcctgtccct tttcatcgcc atcttgccgg ggaacaggaa tgcaactggt
     3061 ctttgggtga ataatcaccc ctgtcccctt tggatctgga gtcgtgaata ccgtaatggt
     3121 gagcgcatcc gattggcgcg ttgtcaaatg gccatatgca gcctgtgcca tcccaatcca
     3181 aaaaaatgca ggcagaatct catatttctg gccctgatca acagatcgag agcatgtatt
     3241 tcacatgaat taaaccacca gatttgtcaa ttggtatagc gaattgattg tcccgcatat
     3301 ttttcctttt ccacatttaa ttctggattt ttctgcgatt gttaaaaccg aattattgat
     3361 atgagttttt atttcacccc tctttctgta atatcaagac ccgcttcatc gattgttggt
     3421 attactcttt atatggtgat gaccatggac agattgcatc gtgtacttag tattgttgta
     3481 ttcactgcac ttattgcagg gatagtgttt ttcactggct gcaccaccag cccgacaacc
     3541 accgaaaagc ccaacctcaa cctctacgtg gcagcaggaa tgaaaaagcc tatggacgtg
     3601 atagtggata aatacaaggc tggatcgacc gggaacgtaa cccccaacta cgggccttcc
     3661 gggggcctct acacccagat aaccctgggg cagccttgtg acctctacta ttcagccgac
     3721 tggatgtaca tagaaaagct ccagaacgag agcaggacat ccgaggtcca caagttcctt
     3781 aaggactata ttgtaatggt ggtctcaaag accggaaagg agaaggggat tacttcgacc
     3841 gcagacctca ctggcgatgg ggtagtgatt gcagttgcgg atccggcagc accagtcgga
     3901 gcatatgcag agaatgcgct caagagcctt ggactctggg atcagctcaa tgcaatgggc
     3961 aacatcaaga cacggcccaa cacagtcaac cagcttgccc tcatggtcca gaacgacgag
     4021 gtcgatgtgg gattcgtata cagcagcact gcgaacctgt atggccttga tgtggtagaa
     4081 aagttcagcc acgaccagac cggcgagata gtgttcggag cagccgtcat caagggtgga
     4141 aacgaagcag ccgccaagga cttcctggcc ttctccacac agaacgccgg tgagttcatc
     4201 gcatacggct gggaaccgta tgcgtaaggt cagtacctgg gaactcctct tcgttcccct
     4261 ttttttcctt ctgattgtct acccgatcgc agcaatcgtg gggaatgtaa caatcgaggg
     4321 catggagagg acgtttgccg atcccatctt ctgggatgca gtgaaaaata cccttatcac
     4381 tgcgttcttc gccgcactcc ttggcgtgct gatggcgctc gggtttgggt acttctacct
     4441 cttccacccc ggcactattg tctacaagat cgcggacttc atgaacgact tcccgattgc
     4501 actcccgcac actgtcgcag gcctcgcact cctcctcgca ttcgggagga agatgttctg
     4561 gtttgcgggc ccgacgggac ttgcattcac cctgcttgcc gtcgcccttg ccatgttctt
     4621 tgtctcgtat ccccttgccg cccggacgat tgctgcaagt gccgacgaga tagacatgga
     4681 cctgatagca gtggcacgta ccctcggcga ctctccggca cacgcgtata gccgtgtggt
     4741 tatccctatc cttggtgaag ccatattctc tgcatacgtg ctcagtttcg cccgatcgct
     4801 ttctgaattt gccgcagtga ttatgtttgg agggaatgtt cctggaatga cccaggttct
     4861 cgcaagttat gtcttttcca aggtcgaaga gggcgagata ggcatggcgg tgaccgccag
     4921 tgctttctgt attctcctct cgctgcttct ggtcctgata ctcagattaa ggagtaaata
     4981 ctatgctcga aatacaggat gtgcataagt cgttcggaga ccaggtcgtg ctcaagggag
     5041 tgaacctcga ggtctccccc ggagagcttc aggtcctgat agggctgaac gggagcggaa
     5101 agaccaccct tctcaagatc ctcacagggc ttcttacgcc agacaaggga cgggtcagga
     5161 ttgacgggag ggacgtcacg ttgttccctc cggaggagcg ttatatcgga tacgtcccac
     5221 agcatacggc gctcttcaat cacctcacgg tgaaggagaa catcctctat tccctccgcc
     5281 ataagagggg atctgaagag acgtttcacc agatggtgga ccttctggca ctggacccct
     5341 accttgagag gaaaccccgc gagctctcgg ggggataccg gagccgcgtt gcccttgcac
     5401 gcgccctgac ctccatgccc agggcgatgc tcctcgacga gcccatcacc gaggtggacc
     5461 gggctaagaa agagcatatc cttccccttt tcaggcgagt gctggtgaaa atgaatatcc
     5521 cggtcctcta catcacccat gacccatggg aggcggagca gatcggcaca tcattctcca
     5581 tcatgaagga agggagtgtg acccggatca attcgcctgc cgaggccttt gcaatcgtaa
     5641 gggagcaggc gctctgtgat gaggaatgag tgagaaggcg tgtggaaacc cttgaattta
     5701 atcaattttt tttctctgat ctcccgtgta tagcatctta ttccaagtac cgacgttaat
     5761 cctgcggcat ttaattgctc tcctgccttt tcccataacc tgtttcttta cccgggattt
     5821 ttaacagacc tcccgccgca tttatctttc caggttcata ctcattcacc atgcacccta
     5881 ccggcgctac gttcatggag atgaccaggt acgagcacct ctccccttct gaccagtacc
     5941 agggcctacc cgcccctcca ctggagaagc ccttcccgcg ggacggaaat ttcatccccc
     6001 ttcccgatcc tgacacgtta caggttccaa tgctcgattt gcgggatgca atagaggaac
     6061 ggaggtcact ccgtcactat acccgcgacc ccctctcgct cgaggaactc tcctaccttc
     6121 tctggtgcac ccagggcatc gtacaggtgc aggaccccta ttacacgctg aggaacgttc
     6181 cctctgccgg gggacgccac tcctttgaga cttacctcct cttgaaccgg gtgacagggg
     6241 tgaaacccgg actttaccgt tacctctcat tctcccatcg ccttttggag atggacacgc
     6301 gtttggggat cgccgaccgg gtgatggccg catgccttgg ccaggcgttt gtccgggcct
     6361 ccgcggtcac gttcctgtgg gcctgcgtag tgtaccggat ggcatggcgt tacgcggagc
     6421 gtgcctggag gctggtgcac atcgatgccg gccacgcagg ccagaacctc taccttgccg
     6481 ccggacaggt ggggtgcggt gcctgtgcaa tcggggcgtt cgacgaccag caggcggccg
     6541 atgtgctcgg gattgatggc gtgaatgagt tcgtcatata ctgtgcagcg attggaaaaa
     6601 aaggcggata attgatggtt caggccatgc ctgccaccag ggaccggacc atttccccga
     6661 ccgcagctct catctcgccg ggtcggccaa gccgatctcc cacctccctt acgattgcgg
     6721 acccgacgat gattccgtct gccccggccc tggaataggc ctgcacctgg cccggcgagc
     6781 tgatcccgaa tccggggacg acagggagcg acgacatctc cttgatcttc ctgatgcacc
     6841 cgatcgtcgc aggatcaacc tcccctctca tcccagtgac gccgagtgct gccacaaggt
     6901 acaggaaacc ccctgctttt gcggtgatcc ccttcaaccg ttccatcgag gtggtctggc
     6961 tcacaaaaag gatggggtcg acccctgcct tcctggctgc cctgcagaag gaatctgcct
     7021 cctcgagcgg cacgtcagcg accgcgaccc cgtctacgcc tgcacccccg gcatcacggt
     7081 aaaaggctcc gatcccccgc tgcaccaccg ggttggcata tgtaaggacg acaagcggcg
     7141 tctccgattc agcccggaag tccctgatca atgagaagag ggagtcggta ttcatccctg
     7201 ccgagagcgc ccgctggatg gcctgctgca tgacgggtcc gtccgcgacc gggtccgaga
     7261 acggcatgac aacctcgatg atgtcggcac cggccctgtc cacctccctc atcatcgcga
     7321 gcgattcccc gtaatcagga tcacccgcca cgaggcaggc gacgagcagg ggacgcgtac
     7381 gggcggaaaa tgcacgcacg atgcgggtgg tcacaggcta tctcctccga gtgccgccac
     7441 ctgagccacg tccttgtcgc cacggccgga gaggtttacg accaggacgt cgtccttctc
     7501 caggtcctgt gcgattctcc gtgcgagcga aacagcgtgg gcggattcga gcgcagggat
     7561 gataccctct gtccgggaga ggtagaggaa cgagtcgagt gcctcgtcat ccgtcaccgc
     7621 gcgataagtg accctccctt tatccctgag gaagctgtgc tccggaccca cgcctgggta
     7681 atcgagcccg gctgagacgc tgtgcgactc caggatctgg ccgaatgcgt cctggatcag
     7741 gtaggtgtaa gtcccatgga gcactcccgg cctcccgcgg cagagcgtag ccccatgccg
     7801 accctccagg ccctcccctc cggcctctgc accaaagaga agcgttccgt catcgaggaa
     7861 cggggtgaag atcccgatgg cattggaccc tcctcccaca caggcgacga tcgcatccgg
     7921 aagcctcccc tccttttcga ggacctgaac ccgtgtctct ctccctatca ccgtctggaa
     7981 gtccctgaca agggtgggga aggggtgagg gcccactacc gacccgataa ggtagtgggt
     8041 gtcgcgaacg ttcgcaaccc agtcgcgaag cgcctcgttg atggcatcct tgagggttct
     8101 cgagccggac tctaccggga gcacgctcgc gccgagcatc ctcatcctgg ccacgttgag
     8161 ggcctgccgg ccgatgtcct cttctcccat gtacacttcc accttcattc caagcgccgc
     8221 ccccgcgatc gccgtcgcga caccgtgctg gcctgcaccg gtctctgcaa taatccgtct
     8281 cttccccatg cactttgcaa gaagggcctg cccgagggcg ttgttcaact tatgcgcccc
     8341 ggagtgaagg aggtcttcgc gcttcaggta gaccctgcac ccgagatccc gggagaggtt
     8401 ccggcagaaa gtgaggggag tctccctgcc tgcatattcg tgcaggtaat agtgcatctc
     8461 ctgctggaag gatgctcccg gcacgatggt ccggaacccc tcctcgagct cctcgagtgc
     8521 cgccatgagt gtctccggga caaaacaacc cccgtaatcc ccgaaccttg tggattcaat
     8581 cataaaccgc tcctttcgca atcctcacga attcccgtac tttgcgttcg ttcttcacac
     8641 cgggcgcgtc ctcgactccc gtggccacgt ccacgccata ggggcggacc cgccggactg
     8701 ccagcgccac gttcccgggg ttcagacccc cggcaaggat gaccgggacc ctgctctctc
     8761 tcaccacgcg ggaagcatac tcctcgtcgt acaccatccc ctttcccgcg ctcccgtcaa
     8821 caaggacagc gtccgcatcg atcggaaggg cccgtccgcc ggcgacgacc cggatgagcc
     8881 ggacagggaa cgggtgaggc agtgcgagtg atgacgatat ctgtattacg tcgggattca
     8941 ggtcaagcat cgcctcgatg tccccaagag atctggtatg cgacacgcac accctggcaa
     9001 tgtaggggcc cgccgcgcta aagatctcct ccgccgtctc gatgtcaaca gaccgcgggg
     9061 agtccgagta catcaccacc ccgatagcgt cagccccggc ctggtctgcc atgacagcgt
     9121 cgccgggaga cgtgatcccg cagatcttca cgcggatacg aatcccccca gcctcttttt
     9181 tgggtctttt gcagacataa ccgaggatcc gatcagaaac ccgtccacaa ccttccgaag
     9241 gctcctgaca tcgtacggcc agacaaaacc gctctccgag actaccctgc acccggcgtc
     9301 tctgatcagc ggggccaggc gccgcgtggt ggagagatcg atgctgaaat cagccaggtt
     9361 gcggttattg atcccgacaa gccttgcacc ggaatcgact gcgtttgcga cctctcctga
     9421 tgtgcggacc tccacaagcg gctcgaggga gtatccggca gccatatcca cgaactcagg
     9481 gagcctgtcc cggaggacgg atgtgatgag gaggaccgcg tccgccccaa gagctctcgt
     9541 ctccgcgagc tgtctctcat caatgatgaa atccttcctg aggatcggga cctcgacatg
     9601 cggcctgatc agggggatca gccccggatg cccgtggaag aaagatggct cggtgataac
     9661 cgaaagggcc gcgcaccctg ccgaaaccat catgcgggcg agctccactg gatcgccgct
     9721 ttctctgatg accccgcggg aaggcgatgc atacttgatc tcagatatga tagcatttcg
     9781 tccatttctc tctcccatga ccccaacaag gctcattttt tgcttgccgc cagtgacccc
     9841 aactgggtat tgttccggga ggccggcgac ccgaactccc gttgatgaga tgatctcgtc
     9901 cagtatcatg acgccctccc ggaggcggtc acgagcgcct ccagttttcc aagtgcggcc
     9961 ccggtacgga tggactcttt tgcgagggtg atcccttccg caaggtcatc cgccttccct
    10021 gcaaggtaga tggcagctcc cgcgttcatc accaccacgt cgcgacctgg gccctccccg
    10081 ccttccagga tgcgcaggac gaccgccgcg ttctccgcag gggtgcctcc ccgaagggcg
    10141 ttcccggatg agacgggtat cccgaactct ccgggcgtga acgtatactc acggatagtc
    10201 ccacccgcaa gttcggcgac ctgcgtgggg cctgtcgtgg tgatctcgtc aaggcccgaa
    10261 ccatggacga ccatggccct ctcggccccc atgagttgga gtgactctgc aagagtcctt
    10321 aagaaggccg gggagtacac ccccatcaat ctcgattgtg cgcctgcagg gttgaggaga
    10381 ggtccgagca tgttgaatac cgtgtggaac ccgagatcct tcctcaccct cgcagcattc
    10441 ctgaacgccg ggtggaatgc cggcgcaaag agaaaaccga tacccgtttc ctcgacgatc
    10501 ctgcatacgc gctcaggctg gatgtcgatc ttcaccccga gggcctccag cacgtccgcc
    10561 gaaccgcatg agctgctcac gctcctgttc ccatgtttca cgattaccac gccggcaccg
    10621 gcggcgacga ttgccgcagc ggtgctgata ttgaaggtcc ggcacccgtc acctcccgtc
    10681 ccgcaggtat cgacgagggt tcctgcaacc cgtggcctta tcgtgaccgc gtaatgctgg
    10741 aggacccgtc cgaaggttgc aatctcctcc gcattctcgc cccttattcg aagggcggca
    10801 aggaacgccc ccatctgcgc atccgtggcc ctaccggacg cgatgtactc catcgcctcg
    10861 cgtgcttccg ggtcggacag cagcctgccc tcgctcagca cctggaggac tgtctgcatc
    10921 atgcacctcc cctgaacagg aagttctcca tgatcaccct cccttcaggg gtcatgatgc
    10981 tctcgggatg gaactggagg ccatataagg gatattcgcg gtgcgtcact cccatcacgc
    11041 accggtcctc ctctgagagc gcaacagtct cgaattcctc cgggaccctg tccgccggtg
    11101 ctgcaagcga gtggtaacgc gtggcggtga aggggttcgg gaggcctgca aagagtccct
    11161 gaccgtcatg gacgatcctg ctcgtcttcc cgtgcaccgg cgcatccatt ctcctgatcg
    11221 cccccccatg gcagtggatg atggtctggt gtccgaggca cacgccgagg atgggaactc
    11281 ttccctcaaa ctcgcttacc acctcggggc atacccctga gtcttcgggg gttcccggcc
    11341 cgggcgagag gatgatgcgg tcaggtgatt ctttcctgac cgcggaaaga gtccggtcgc
    11401 aggtgacggg gacggggact gccccgagca tccccaccat ctggtagagg ttgtaggtga
    11461 agctgtcgaa gcagtcgacg attaccaccc tcatctcccc acccctgcct ggcgtatcgc
    11521 cttttccatc gcccctgcct tgtacccggt ctcctcgaac tcacgttccg ggacagaatc
    11581 cgccacaatc ccggcgcccg tcgagaactc caccacccct tccctcgcga cgagcgtgcg
    11641 gatggcgatg gcaaactcaa gggcatcatt gaaaccgata tacccgaccg cccctgcata
    11701 gagcccccgc gggtgtgctt cgagatctgc gatgatctgc attgcacgga tctttggcgc
    11761 accactgacg gtcccggccg ggaaacaggc tgcgagcgcg tcgaagcggt cgcagccgtc
    11821 acggagggtt ccgcacaccc tggaaacgag gtgctggaca tgcgagaaac gctcgacctc
    11881 catgaactcg gggacctgca cagacccaaa tgccgacacc ctcccgatgt cgtttcgggc
    11941 caggtcgaca agcatgaggt gctccgcccg ttcctttgga tctgcaagga ggtcggctgc
    12001 aagctggtca tcctcgcgaa gatcgctccc acggggcctt gtccccgcga tggggaccgt
    12061 ctggacggct ttcccctcga ccttcacgag catctcgggg ctcgacccga ttaccgtctc
    12121 gtccccgaac ctgatgcaat aaaggtacgg ggacgggttg atggtccgaa tcgccccgta
    12181 gaggaggact ggatcgcctg tgtacgggca ggagagcctg cgggagagga ctacctggaa
    12241 gatgtcccca gccctgatgt gctcctttgc tttcctgacc gcggtcatga actcttcacg
    12301 gtccatggtc gtgcgaaggg gaagttccat tccaccccca ggcccctgtt ctcctgggtc
    12361 agccccggga actgccatga cggcctcctc gagcaagtcc atccgctcgt tcgccctgtc
    12421 atacgcctcc ccggtgtctt ccccctcgat gagcaggggg tcgtcaaata aaagacagga
    12481 gccatgcaca tggtcgtaca cgatcccgcg ggtgcagagc atgaaccgcg cgagcgggcc
    12541 ttcctttcct gcaggcacaa ggcctgccga gagatcggtc accatgtcgt acgcgcagta
    12601 tcccacgagg cctcccgcaa agccggtgac acctcccccc tcgcagcgga accggttgca
    12661 gagtctgcgc aactgctcga cagggtccga tccttggggg aaggaatccg tattggagag
    12721 cctctggtcc cccttacagg taaccttttc atcgagagtg aagacaagtg gcggctcgag
    12781 gcccacgatg gacctgacag ccctccgggg aacgccctcc atcgattcga gtatgcaccc
    12841 cgttcctgcc ccgaggccac gaaagagggc gtacgggtct gcggcgggga gcgggatacg
    12901 acgtgtgacc gggaggacca cgatgggccc ggcatttcgc cctgcaggta tcagtccttc
    12961 gggacgatct gaatatgccg ccgcgaaggc ggcaatcccc attccggcgg catcaccagg
    13021 ggttgttcgt tctcttttat catgtttatt tatgtacatt attgattata tatatacatt
    13081 ggtgttaatt ttcgagaggt gtataggtat cccctggacg aggccggtct ggtaaagaca
    13141 ctcgcaactg tccggatcac tacattcctc atggcggcat tgccggagac accgcacgga
    13201 aatgtctatc ccttcggtat ccaataacgc cataccacga aggagaagag agtatggtca
    13261 caatcgtgca tttcgatatc ccggcggata tcccagacag agcaaggaat ttttatgccg
    13321 cactcttcgg atggaagttc accagtccac cgggctaccc cgacttttcc ctgatcgaga
    13381 ccaccggttt ggaaggtcat ccgggtgttg gcgggggact cgggaggaga ggagagccgg
    13441 ggcaggatat cacgctgtat ttcggggtgg aatcagtgga gagatatctt gcccttgtca
    13501 ttagggaagg ggggaaagtc gtgatgccct atacccctgt ccccggatac ggcgcgctcg
    13561 ccatctgcac cgacaccgag ggaaacaggt tcggtctctg ggagaatacg gcgtagccct
    13621 ggacccatac attcaaggca actgcagggc tttccttcta ctggtataca tggcagcaca
    13681 gagacccgat ccaatgactg gccagcctgc tccctcgttc tgcctgccgg actcgaaggg
    13741 aaacgaggtg tgcctcccgc agttccgcgg gaaatgggtg gtcctctact tctatccgcg
    13801 tgacaacacc cctgggtgca ctctcgaggc cctctcgttt cacaatgcgc tcgagtcgtt
    13861 cgcagggatg ggcgcacaga tcctcggtgt aagcgcagat acgccggaga gccaccagcg
    13921 gtttgccgac cgccacaacc tctccatcct gctcctctcg gacacaggcc accaggtcat
    13981 cgaggcctac cattcatgga agccgaagaa gatctttgga aaggaggtcc tcggcaccca
    14041 gcgcgattca ttcctgatcg accctgaagg aagggtggtg gaggtatgga ggaaagtatc
    14101 cccgaaaggg cacgcggatg aggtcaggga agcccttgtc ggcaggcttg gccgttcctg
    14161 aaacaggagg cagggcctct cccccgcccc attcttacct cctacccacg aacacgcaaa
    14221 aagtccggtt cttgtagaca acatccacac ccgagaaacc ggcctccgag agccaggaga
    14281 tctgatccag aagtgttgca ttcctgtcaa gcgtgtccct gcgtgtcatc gcggctctca
    14341 gttcatccct tgggaaaggg ctggcactga caaacgcatc ccagtattcg atattcctcc
    14401 ttgagagcca ttccgactgc gcctcggcct gatcggcgtt gacaaagaga cccccggagt
    14461 tcagggcttc gaatatccgg cggaacaacc tgcgcttgtc accgtgctcc aggtggtgga
    14521 tggagagtgc cgagcagacc aggtcgtaac ggcctggcag ttcctcccgg ctgtaatctg
    14581 ccgccacgaa tcgtacccgc gggttcccgg caaagcgctc cgtcgccacc tccagcatct
    14641 tctccgagat gtcgagaagg gtgagggtcg cacgtggata cttctccagg aggaatgccg
    14701 aaaggaggcc tgtcccggca ccgatgtcga ggattgaagg gctctccccg ggccaggccg
    14761 cggcccagac cgccgcctcg tagaactcct ccatctgtgg gatcacaaac ctgcgctggc
    14821 gatcataccc ggaggcaatg gcatcgaatg cctccctgac gttctccatg aaaggatgtt
    14881 ggaatggcca taccataatc ccgttcccct tgcacaggaa acaaaggatt actcccttca
    14941 gaagcgaagt ccctgccggg atgcgtccca cgctgcccat gcaccgaaac agacatgccg
    15001 cgtcccttcc aagctgcatg gagaatgacc atcggtgatc cggccgggta tgtccacggc
    15061 cactcggagc gcgaggctgt ccgcctctcg gaccaggcct gcacgcttga ggagctcctg
    15121 caccacgaca ctttttatcc tccccaatcc ctggtcctcg aggccggctg cggcgtgggg
    15181 gcccagaccg ggatcctcgc tgcaaggagc agtgaagcgc gcatcgtctc gctcgatctc
    15241 tcgctctcct ctctctcgtg cgcaagggca agcgccgccg ccggtggatg ggaagaggtc
    15301 acgtttgcaa ggggagacct ccactccttc ccgttcagaa atggctcatt cgaccatgtc
    15361 ttcatctgtt ttgtcctcga acacctccag gcccccctga ttgccctcca gtcgctgcga
    15421 gaggtcctga aacccggcgg aaccctgacc gtcatcgagg gggaccatgg atcggcattc
    15481 ttccaccccg agagccggca tgcatcggac gcaatcctcg ctcttgagag cctccagaga
    15541 tattccggtg ggaatccacg tatcggccgg gagctctatc ccctcctcac ctcgtcgggg
    15601 ttccatgatg tccacgtctc accgcggcag gtatacgtcg acgactcgaa acccggcctc
    15661 gtcgaaggct tcaccaggca gacgttcact gccatggtgg aaggtgtccg ggagaaggtg
    15721 ataacctccg gaatgatgcc ggaggaggac ttcgaccggg gggtccgcga tctctaccgg
    15781 acaagcgaag gtggcggaac cttctgttac acgttcttca aggcggtcgc ggtaaggtga
    15841 gatgcgagag gtggagcggg tagtatttcc cgaaatttcc gggagaaccg gtgagcgagc
    15901 cgtttggatt tacggcgttg aagataggtc ctttggattc atatgggaat ctgggttttc
    15961 cctttccaat acgtgacggg atattcgcct attcgatcct cctgagaact gccatgagat
    16021 aagaggcatc ctttgatcca aaacgggccc tgctccactc gatctcccag tccctcccgt
    16081 cgcagcctat gacacgacac gatgtggtct tctccccaac cattgtcaaa tcggccatgc
    16141 tccgcctgac ctcttcctcg ccgccgggag agaggaatga ccagagcggt ttgccatgca
    16201 caccccccgt gcttgtcttt ggcgaaatgg agcgggcagg cttgttggca tacagcatgg
    16261 tcccatcggg tgtcgtgatg accgtggcat gaggcgtcag gttcaggaca gcatcaagga
    16321 cctgctccct ctccctgcgt gccatctctt cgcgcttgag atcggtgaca tcacggaata
    16381 tcgccctggt atactgtggt atgccgtttt ccatgtggca gttcacgttc ccttcgacgt
    16441 acaccttcct gccggtcttc gatcggaacg tggcttcgat gcgacccacg tcctccccgg
    16501 tcatcacccg cccgaaagta atcctgcagt gctccatcga tgagggatcg atgatatcga
    16561 agatattgag acctgcgatc tcctcaggac tgtattccag ggtctttctc cacgcctggt
    16621 tcacgtagag aaaacgcccg tcggggccga cactctggat gaggtcgttc gcgttctcca
    16681 ggagatcctg gagccgaccc tcgctctcgg caagctctgc cctggctgaa gagagcttcc
    16741 gaagggtatc cagccagtgc tgcagttcaa gggaagggag gttgtaccgc aatgcatcca
    16801 tggcagggat gtagaagagg ttctcgacca cccttccccc gaggagtatg agtgggtggg
    16861 tccgcaggac atcctggagt accggggcgg gaaagagatc catccggtat gcacacagga
    16921 gaaggaggct cttttctttg aagagatgat cgaggcctgc catgtcgcct acgaggtgct
    16981 ggctggagcg ccttgccgag ataggcggca cctcgcgcat gatgcagaga gcggggctgc
    17041 ccgccgatcg tgccatcctg accctttctg tgataaatgc cttggcattc ctggcggtgg
    17101 caaagtgcct gtgaaactcc tcgtcctggt gggagaggac ggtcacgttg ttctcttctg
    17161 caaccctccc cgtcttccct atgataaggg ggttttcatc cggcaacctg gaaagataga
    17221 ggagatgcca cctgttcctc ctgcactggt ccagaatgga ccctgtcacc tccacgatct
    17281 ccccggactg ctggtaaagt atggcggcat gatctccagg tgccaggcca ccctgctctt
    17341 taattctcgg tgtgttcatc ccatccccct cccgcgccac aagccccggt atccatggcc
    17401 tgtcacggat aggtacgttc agaaaggtag caccctcatg aaatactttc cggggcactc
    17461 cctcccgcct gtgccaggaa aggtcactct ccccccacct ggctcgtccg ttccggtcct
    17521 tccacccggc caatccgttt tctctgtgca atccggtacc cggacaccac ggagagatgg
    17581 tcaccaaatg ccagatccat gtccctgtcc cccgaatcta cgaagaccat gggggtctcg
    17641 gcgagcttgt gcggtgtggc aacgatgatg acgttttctc tcccgatcct tcgaagaact
    17701 gccgggctca cctgctgagt gccccttccc agcaccgagc cctgtgcacc gatgatgctg
    17761 atgacaaggc gggcactggt tccttcttca agaagcccca gtaatccctc ctcgtttaaa
    17821 tccgtggcga tgagccttcc tcctttcacg gcatcgaagc cgaggagggt ctttttcagg
    17881 ccgatctcct ccccgatggc cgcagtggtg ctccccgggc caaggatgta gagaataccg
    17941 ggggtccctt cgatcacctc ccgcataaac cgtgcaatat ctttctttgc acgttcctca
    18001 tcagaatcct cgaacacctg ctttgtcccc tgcacgaggc ctgggcggta cgggctcttt
    18061 gcgataccaa agaggcgggt ccgtagcgat ccttccctgt atgcttcctc gtccacgtcc
    18121 accacctccg catcacggag gagaaacttg cctcccggtg tgcatccctc gcgcactatc
    18181 cctgcggctg cgcgagggtg aatggcaaag accgccgagt acatcttcac ccctgcgggg
    18241 attccgagca tggggaccaa gtcccctatc acggaatata catccctggc agtgccgtca
    18301 ccgccgcaga agaggatgag gtctgcatcc ctgtcaagaa acgccctgca tgctgccatg
    18361 gtatccgctg ctcccgtcgt ttctgaagcg ggacgatata ctacttcgta tcccgggatt
    18421 cctgctcctg ccaggatatc ctcccccatg accccggaac aggtaatgaa atgagccccg
    18481 atgtccgcga cctgcaatat cgtctctgcc gccctccccg gagcccgggg tacggcaccc
    18541 ctggcaaggg ccgcctcaac cattccatcc gtccctttca ggcccactgc tccgcccata
    18601 ccggcaaagg gattgaccag gaaccctatg gtgcacatgc gcgtgaaata actgatggag
    18661 gaaggggtga tatgtgttgg ggtactaatg gcaaaaaatg ggggagtctt ttgtggtaac
    18721 ccattctcct gtcatgcgcc cgggatcttc gggagacggg caagggactc gcgtatcagc
    18781 tgctcgggat actcgtaatc cacgagctgg ttggcataga acgcatcata cgcagacatg
    18841 tcaaagttgc cgtgccctga acagttaaag aggagggtct ttgcctctcc gctctttttg
    18901 catttgagtg cttcatcgat ggtcgccctg acggcatgcg aggtctcggg agcgacgata
    18961 atcccttctg tcctggcaaa cgtctgtgca gcatcaaaga cctcgctctg atggtacgag
    19021 accgccttca tcaccccgtc atacacgagc cgggagattg tcggattgtc gccgtggtag
    19081 cgtagtcccc ctgcatggat ggagggcggc acgaagtcat ggccgagggt gaacatcttc
    19141 aagagcggag tcagtccggc cacgtcacca tagtcatagg caaagagccc cttcgtgagc
    19201 gtcgggcagg atgccggttc caccgcgatg atctcggttt ccttatgctt tcctttcagc
    19261 ttctcaccgg caaacgggaa cgagatcccg gcgaagttgg accctccgcc cacgcagccg
    19321 attaccacgt ccggataatc ctcgacggat gcgagctgct ccatgcattc cagtccgacc
    19381 accgtctggt ggaggcagac gtggttcagg acggagccaa gggagtaatt ggtgttcccg
    19441 tgagtggccg catcctctac tgcctccgat atggcgattc caaggctccc cgtggtctca
    19501 ggatccttct gaagtacggc ggttccggcc ttcgtcttcg tgctcgggct cgggatgcac
    19561 tctgctcccc atacctgcat catcgacttg cggtagggtt tctgcatgta actagacctg
    19621 accatgtaga tggtgcactc aagcccgaag atctgggttg cgaacgcgag ggccgagccc
    19681 cactggcctg cgccggtctc agttgcaatg cgctcggtcc cggctttcat gttgtaatag
    19741 gcctgcggaa ccgcagtgtt gggcttgtgg ctcccgggtg ggctcactcc ctcgtacttg
    19801 tagtatatgt gggcgggagt cttcagatac ttctccagac ggtgagcgcg atagagcggg
    19861 ctcggcctcc agagcctcaa cacgtcctgc acatcggaag gaatatcgat ggtgcgtttc
    19921 tggctcatct cctggaggat aagttccatc gggaagatgg gggtcaggtc ttccgggaca
    19981 agcggttttc ccgtggccgg gtgcagcggg ggatcaagcg gggtagggag gtctggcagt
    20041 atattgtacc actttttggg catgtggtcc tcatcgagaa cgatcttggt gtgcatactc
    20101 atcgtatgcc gtgtataaat atatacattg ttgtacaatt atgattattg gtggcgggta
    20161 ttctggtgtt ctaaaattgc aaatatctcg aatatgggtt attcaggggc cgaggaggtg
    20221 ggttgcctct tcctctgaat cgcagatggt aaatatcctg gagaacccgg tcaggtcaaa
    20281 gacctcctgg acaaatggct tcatggcgca gaggacaagg ttcccttccg cctttttcag
    20341 ccgtttcatt gccgcgagga ggactctcag gcctgcgctg ctcatatagt ccactgagga
    20401 gaggttcacc actatcctcc tgtgcccttc atcgattgcc tttgagagct ccccctcgag
    20461 cattggcgcc gaggccgcgt cgatcctgcc gcagggcgcg atgatgcggt gccctgccgg
    20521 cacaggttcc tccatcctgc aggggacttc gcctattgcc gtatccgaac tctttccagc
    20581 cgcgggatat tcaatgaata cagtggtact cccccgtgtc gaagatacgc tgcagctccc
    20641 gttggagagg ctgcagcttc tcgccgcgaa acgccgcacg ttctcttccg tgaatgccga
    20701 ggatgccggg aactgaagct tcacccaaac ctggtctttc gtgcatccgg ttgcaatgtg
    20761 caggctctgt gctcctgcaa ccgagacctt ctcgaggata tacaggaaca tgtccccgaa
    20821 gcgttcccgg tccatcatcg caggcggcag gtttccgcca ggagaatact ctatcttcag
    20881 gtgctcgaat gggttgacgt acgcaatcct gactgcaagt gcccgggcaa actcctcctc
    20941 tgtctgagcc tcgatgatgc cttcctcctc gtaggggggg cgggaaatag ccgcaactac
    21001 ctcccctatg gcagcagaga ggtcggtcgc ggcaagatcg atccgcggcc ggaatcccct
    21061 gaacgtgaca gtgtaatcct cgagcagcct gcctacccgg ctcacgagag agagatcgag
    21121 caccgatccg agcatctccc cccggtatac cttctcgagt ttctgggaga tctggttcgc
    21181 cttgagcgcc aggtgtatct cgagcgcctg cccggagtgg aaatcaagag agaacctgtt
    21241 gaaatcagag aggatctcat cgacaagatc gctgttgatc agcccccgga acctctccgc
    21301 ctcccccgac tcttcgagct cgtcaagcac atgggccaca tacacgagcc tgccggcaat
    21361 gatcgtgaag aggcgatcca cctcacccat cagctctccc gcatatgccg cggtatcctt
    21421 cacccactca ggattcacga tctcgatccc ctcgaggcgc gaggcctcct tcttcattgc
    21481 ctggagcgtg cgggtggttg ttgcggcatc cagcggacgt ccgtgccctg gaaggcagac
    21541 ggtgattcca cccttctgca ggagcgcgat cacctggtcc agggtgaaga ggagagcttt
    21601 taagtcccat cccttcagtc ctgcaactcc cggtgcggtg gagaagagga ggtctcccaa
    21661 aaagagcgtc tcccccatac ggatgcagat gctgtccggg ctgtgaccag gtgcagggta
    21721 gaactcgatc atcgtatctc cttccagcgg cagcgtctgc ctcgatatgg tgagtcctcc
    21781 tcccagcgga agggtgtcca ggcaggtgat caccgaagac ccggcgatgt tgagtatttc
    21841 atccctgtgt tctcccccgt ttccggatgg caggagcctg atatcgatcg ttatcgtccc
    21901 cacctcctgg cgaatcaggt ccgcgatggt ggccgcgcgg tcacggctct ccagcgcccg
    21961 cgcacctgcc tcctggaccg cgaccagcat gtggggaagc gtccggaacc ccgggtggtg
    22021 caggagctgg tggcagtggt cgaggtggac gtgcgtaagg cagatcaaca ccggtcgcgg
    22081 cttttctgtg atcagctttc gtatctctcc aagaagagta tctgcctgcc ccggaaggcc
    22141 accggggtcg accaggacga tgagtcgtgg agctgccacc aggtacgagt tcgaggatat
    22201 gatgtccacc ttgcggagga acggatagat cgaaacaccc tccacaccgg ggaggggctg
    22261 ccatatctgg ctctccatat cagccaattg caatcacctg cctcatccac tgcacacggg
    22321 gaatccacgc gtgcgatcat attctcttcc ccaggagctg atatctgata ccctgatatc
    22381 aggagtgccc catgcaggag ccagacgacc gccagcccat ccggttctcc gcgccggtga
    22441 atgaccagtg gaactacctc ctctcgacga tatggaacca gatgatgcac ctcgttgtca
    22501 tacttgatgg ccggcttgac gaagaaaggc tccgccatgc tgcggagctc gtaatggagg
    22561 ctgagcctgt cctggcctgc aggtttgtcg aggctgcggt gccttactgg gaatggattc
    22621 cctctctccg gtcggaatcg gtcttctcaa tcgccgggca gggaagcatc tgtgaaacat
    22681 ctctaaaagg cgcgctcgcc cgaaagtatg accctgcagc aggcccgcag gcacatttca
    22741 cccttcttcg tggagacagg gactcactgg tcatctctgt caaccatgcc gtgtgcgacg
    22801 cccgcggcat cctgcacata tccagccttt ttgcccgcgc ctaccgggca cttgagagca
    22861 attccgggtt ctccctgtcc tgcgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22981 nnnnnatacg gctcgaagag acgttctctt caggatttca ctccgtgcgg gagcatctcc
    23041 tcgatgcaaa acgccgtagc ctgatcgaag gctacccaaa gaatcctttc ttcagcaata
    23101 tcggaatcct gcccatcccg tgcgcggact tcaaggatat cccgaccagg caggcgttca
    23161 ttatgcctcc cgttgagtat tccccgggct tcggtatcac tgccagtacc ttcagagaca
    23221 ccctcacgct ctcgtgtggc ttctgcgaag gcccgttaaa gggtgggacg gttgagaggg
    23281 tgctgttctc gatggaagag ttcatctcat cgctcaccag ggagtgagaa cagcccagtc
    23341 agggaggccg ggcgggatac gaagcgttac aggcacgatg gtctacatcc acaggatgac
    23401 cgggacaaca cctgtgacat tgagggtaaa acctgcacgg atcatgtccc ttggtctttt
    23461 gtgcccggtc ccgtatgtga tggtactggc tgcggcggct ccccgcacgt cgtgaggggc
    23521 cccctctcct ggtggggatg agtacgagga aaaatactcc aatcccggcc atcaacccga
    23581 ttacacggcg catgtgagag agaggttctg aaaaatctct cttctcacct gcgattagag
    23641 agggagcagc cgggcaaccc tctcgccggt ctcgtagatg gcgtatgtcg gattcccggt
    23701 aagatagccg catatctcgc caggattgat tccaagcgtg cgttcctccc ggtatatcga
    23761 gcactggtga gtgtggcctg aaacgacgat gtcatacttt gcgctcccga tgcactcggc
    23821 aaggagcgcc cggtcgtggc cgtgcatcag cgcaacacgc tcgccatcca cccggatgtc
    23881 ggcaaaaaat cccgacaact cgatattctg ctgctcgcgg gaacgcgtcg ccagcagttc
    23941 gcgatccccg tcgttgttcc cgaatacccc aaccacccgg accgggatcc ttcccaggag
    24001 agggataacg aatggcgcga tgtaatcccc ggtatggagc accatgtcaa cctcctcctg
    24061 gataagcgcc tccaccgcgc gcccgatgtt ggggaggtga tcgtgggtgt ccccgatgat
    24121 gccaatcttc atctcatgcc tcacatcctc aatattggca ggactacata aaggaagcat
    24181 tattggtgca aaaaagccat ctggagagga tgaccgggcg cgaggacgag agggagatcg
    24241 tatgcggact tgagcgggcg attgtcggca atgaccgcat tgcacgacgt aggctccttg
    24301 accgcctggt ggtgctcctc ttcaccaccg ataccgccgc accggcgctc gtctcccatg
    24361 gtggaatccg cgtcctcctg accgctgcaa gagacaggga cgagaagatc cgcctgcacg
    24421 ccgtccatgc tctttccagg ctcgcagatt ccggctttgc gtcagagttg agagatgccg
    24481 gtgcaaggac ttccatcgcg cccctcatgg aagacccgta ccttcccctg cgggccatgg
    24541 cagagaagct tcttgaaaaa attgacggtt cctgacatac cgactgtgcc agcgacaggc
    24601 gccggccaat gacaccacat ggctttttac acccgcttcc ggcgcgaatg cttctttagg
    24661 gttccactcc acacgccacg ggccagtgtg aaccaataat atggatcggc cataatcctt
    24721 cgtttatgaa gatgaggatt ggcggcgtgg aggtggcagg agccgacgaa cgctggatgc
    24781 cggtcgtcaa cccggcgacc ggagaggaga tcgaccgcgt accggaagga accctctacg
    24841 aagtggacga tgcggtaaaa gcggcaggag acgcgttctc ttcctgggcc ggacgggctc
    24901 cgagggagag gggcctcctt ctcttcaggg gtgcggcact cgtccgggaa cggcacgaaa
    24961 atctcgccag gcttctcacc atggagcagg gaaagcccct gaaggacgcg gttgacgagg
    25021 tcaggggttt tgccaatgtc ctcgagtact atgccgcgct ctccggcagc ctcgcaggag
    25081 agtatatcca cctagggaaa gcgggcgatg ccgtggtggc ccgggagccc tttggggtct
    25141 gcggggcgat tgtcccatgg aacatgcccg cacttcttat ggcctggaaa gtggcgcctg
    25201 ccctcgtggc aggaaacacc atggtattca agcctgcatc cacgaccccc ctcacgagca
    25261 tacgcctggc ccatatcctc gatgaggcag gtcttccccc tggcgtcctg aacgtcgtca
    25321 ccgggcgggg agaagtggtg ggagaggcac tggtccgaca tcccggcgtg cagaagatct
    25381 cattcaccgg cgacgccgag actggtctgc agatccagga gctcgcatcc ggctcgctca
    25441 aacctgtgac actcgaactt ggaggctcgg accccatgat cgtctggaag gatgcagata
    25501 ttgaaaaggc cgtggcggga gcggtgcgcg ggcggttcta caatgcgggc cagacatgca
    25561 cggcagtcaa acggctcttc gtccaccagt caatcggtga agagttcgtc cgacgtctca
    25621 tggaagcggt aagaacgctc aaggtcggaa acggcctctc ccctggtgtt gacatgggac
    25681 cattgagcgg tgcgccccag cgggagagga taggcacgct gatggagaca cttaaaaacc
    25741 gcggccaggg caaggtgctg acaggaggga tgcccctcaa aggaccagga tacgatgccg
    25801 gttattttta tgcccccaca ttagtgaccg acgttcaccc cgaatccgcg ctccttacaa
    25861 aagaggtatt cggcccggtc cttcccgtga tatgcattcc tgaccttgat acagcaatca
    25921 tgcacgcaaa caacaccagg ttcggcctcg gagcctcggt gtggacccat gatctctctg
    25981 ttgcaaggga ggtcttctcg cgcgtgcggg caggggtggt ctgggtgaac cggcacctca
    26041 ccctgccccc cgatatcccc ttcggcgggg tgaagacttc tggtattggg agtgagaacg
    26101 gaacccaggc actctcttct tacacccggc tgcggtccct ctatatcggg tggtgaaccg
    26161 cagggatgcc gtgaggccct gccactcctt ttaaaaaaac cctgcagtcg aacgggggat
    26221 tgtcgatgga cctcctggat tcccaccacc ctgctcccgg cacccggatc cattcccggc
    26281 catccagtgg ggcctgcagg aaataacccg gggaggtctg caggagcctt catatatcct
    26341 tccgccttac gtctcttccg gtagacattc aaatgactgg agaagagatg ctcaaaatca
    26401 ccgccgatga actggaccgg ctcctctcgc cggcccatgt cgtcggccag gcagtcgacc
    26461 ttggcgacaa ggcggtcatt cccgttgcgg aatttggatt cggcatgggt gcaggaggag
    26521 ggaaaggaaa ggacggtgag ggtgaaggtg ccggatgcgg tggaaagatc gcccccgtag
    26581 ccctggttct ggtctacaag gatgtgaagg gaccggatgg aatccaggtg ctctctttgc
    26641 accgggacaa ccctgttgcc cagattatca atgcgctctc cgagtctctc gccccgcagg
    26701 taataaaggc catcagggcc atgtcagaat cacaggagaa gaaacaggaa ggttgagaca
    26761 gggcacgttg catggatctc cacccggtga tcctggtcgc cgccatcgtc ctggccctcg
    26821 tagtgctgga gctctgcctc tatgcgatcc ccctccggct cagggtgcga atggactatg
    26881 ccaacggcgc gggaatggcg ttcatctcgg cctgctggct gtgcctgggc gtccgaagca
    26941 cgctcgctcc cgagggacga acatttcatc tcctcctggg agacctcccg ctcccactgc
    27001 ccgcattcct tggatcatat gctcccggcg gccggcggcc gggggatatg gctttgcagg
    27061 aaccagagga cctgccggca tcgtcataca tccaatccgc agtcccgtat gtcccttacc
    27121 tgatctcgtt tgccagggcc gctgtacggt ctctctcttt caggcggcta gactgcaggg
    27181 cagtggtggg cctgtcaggc cctgcagaga cgggaatgct ctatggatat ttctgggcgg
    27241 caaaacccct cctctgcagt tcggggagga tcaacctgga aatggtcccg gatttcacaa
    27301 gggagtatct tgaaggacac cttgaactgg acgtcaacat ctcctatcct ttcaaccttc
    27361 tcgcgaggct gattcgcctc ttcacactcc tccaggagaa taaaacgcaa tccaggaaga
    27421 gcggggtccc tgcatgatga tgagcaggga gttgcggata gggccggcat tgaaaacggg
    27481 agacaggatc gtgcttgcaa tcttccgcca gacaaaaatg acgtggccgg gggggtgtac
    27541 agggaactgt acgccggttg ccctggtgat cgtggaaggt gaggagtgca ccgtgtggca
    27601 actgggtgga gacgccgcca tggaggagat caaggataca ctcgcgggaa ttgccgaatc
    27661 tctcactttg gatgcgccaa tcacccgatc gtgcctgtga tcctcttcct tgtatcggcg
    27721 atttttcagg gatctgaagc agtgtccggt cgcaaaatac catgtcccta aaaaaatggt
    27781 tactcgtgtc ttaatgcttc tattgggttc aggttcgatg ctttccatgc cgggtacaac
    27841 cctgacagca ggctcgtccc cactccgaat gccataccat agaagatata gacaaggctt
    27901 gacggcacga acaggtactc tgtggtctgg agcatcacga tgctcacggc gtatcctccg
    27961 aagatgctca gcacccctcc gaggatgctc ccaataagcc cgaggatcag ggcctcgaag
    28021 agaaacatgc tccggacctc ctttcgcatc gtgccgatgc tccgcaacac tccgatctcc
    28081 ttgatccgct ccgtgaccga catcatcatg atattcagga tgctcacccc cgcaacaata
    28141 agggagatcc cgccgattgc cgtcgtgaac gtggagatct gcccaaacgt ggtgagaatg
    28201 gtctcgagga ttgccttggt atcgatcacg tcgacttcgg tctcccggcg gttcagctgc
    28261 ttctcgatgg cctccttggt ctgctcgatc cgggtgaggt ccttgacctt cacgatgacc
    28321 atgtcgtaat ccctctggtc gtacgcctgc tgataccact tgtcctccac cacgatccca
    28381 tagtcggggt tgatgtcaaa gcccattccc cgttccgcga ggatcccgac cacccggagc
    28441 gagcccttgt cccggatcgt gatgcgggac ccaaccttca ggttgttgtc ggcggcaaac
    28501 ttcgcacctg ccatggcccc cgagctcccc cgcaggaatg ccccttcctc gagctcgagg
    28561 aggatgggga cgtcgtccgg tttcaaggca tataccaccc ccaccaggtc ctcagtcccg
    28621 atcctcatcc tcgcagtccc agagtagacc ggaatagtga gatcaggcgc aaccgctctc
    28681 ctgatctgct ctaccttgcg atcggtgatc agctcgctgc ttccacccat ggacgggccc
    28741 atgccacctc ctgcgtgggg agttacgatg atgctgtctc ccaccgtcga aagactgtcg
    28801 gagattgcga gcacgaggct gttgccgaga atccccatgg aagcgatggc aaccaccccg
    28861 atgatgatcc cgatcaccgc gaggagtgaa cggaaccagt ggagccgcac gttcctgacc
    28921 gcaaaatcaa aaaataagct tctttgcact aatcatcacc agtccgaccc gctcgcccga
    28981 tctcctctgc cagataagtg gtatgtcccc cgtgctatat cattctcatt catagcgcaa
    29041 tgcttctatc ggtcttatac ggactgccct gactgcgggg taaagccctg agaggcagct
    29101 gaccaggatg ccgaagaaga caccaagaac gatatatccg atcccctgca ggtcaaaaac
    29161 cctcacatcc ggcacggtgc tgaaggaaga ggtgagcgtg tccgcaacca ccacactcag
    29221 gtaatacccc cccgcaatcg agagcactcc tcctaggata cttccgatag atccgatcac
    29281 cagcgcttcg tagaggaaca ttcgcatcac ctgccctctc aacgtgccga tactccggag
    29341 gattccaatc tcctttgtcc tctcagtgac tgagatgatc atcacgttca ggatgctgac
    29401 cccggagaca agcagggcaa ctccgccgat cccgagaagg aagagggtga ttgcatcgta
    29461 ggtggaatag aacaggtcca ggatctccct gctgtcaagg atatcgaacc tctccctctg
    29521 gcggttcacc tggaaatcaa tatcctcctt cactgctgcc atgctcccta tgtccccgac
    29581 cttcacgagg acacgggtat agccgggatc gggatgccgg tcagcgaacc agccatgcgt
    29641 gaccaccacc gcataatcag ggttgatatc gatggccatt cccctctctt cgagaactcc
    29701 caccaccctc gctggctcgc ccccgacgtt gatgatatgc ccgggggcga tgtcgtactc
    29761 ctctgcgagg agcgctccca ccagcacccc gacaccggtc tccctgggaa acactccttc
    29821 gcgcacagag aggagcagag ggaggtgctc ggacttcaat gccagcagga ttgcatagcc
    29881 ccccctgtcc ctcacttcga ggcgctcgcc ggcctcgacc accgggatca cctggtttgg
    29941 ccctgctgcc ttcgtgatgc gggccaggtc gcgctctgcg atgcctgcgc ccaccgctga
    30001 ccggggatca aacgggtcac cgctcgccgc ggcaaggtgg ggtgtgatga cgagggtatc
    30061 gccgacatcc gtgatcatgc cagagaagag aatgacgagg ctgtttccga gcagcccgag
    30121 cgatgcgatt gcaaacaccc caatcatgat cccgatgccc gcaagaagcg agcgcaccca
    30181 gtgccgctgc aggttccgca tggccagtga gatgagaatg ccggccatca ccgcatcact
    30241 cgtggcgcag ggcctcgatc ggcgtcatcc gcgaggcctg ccaggctggg taaagtcctg
    30301 agagcgtgct cacgatcacc ccgaagagga cccctccggc gatgaagacg aatgttcccg
    30361 gcccaaagac cgaggcgctc tctccgaacg tggtccctgc tgtcatcaca ccgatagcca
    30421 ccttgctgat gaaatatccg ccaaccaccg acaggatccc gccgattgtg ctcccgatta
    30481 tcccgagcat catcgcctcg tagaggaaca tcatgagaat gtccttcctg gatgctccga
    30541 tgctcctgag gatcccgacc tcgcgtatgc gctcggtcac ggagatgatc atcacgttta
    30601 agatgctcac cgcagcgacg agaagggata tagccccgat accaaggagg aataccatga
    30661 tctggttgta aatctggtcg tattgtcgca gcaggtcacg tgaatcatag atgtccacca
    30721 ccttgtccct acggttcagg cgctggtcga tctctttctt gagcgtgtcg atctgggaga
    30781 tgtcgctcgc ttttacgatc acctggtcat actccttctc atctccgaac gtctcgaaga
    30841 accactggtc cgtaacgacg atcgcatagt ccgggttgat atcggcggca tatcccctct
    30901 ccttaagtat tcctgatact ctctgggtct gattcccgat accgacccgg ctccccgggc
    30961 ttataccaaa ctcccttgcc aggtagtatc ccaccaggca cccgggggcg ttttctttga
    31021 gatacaagcc cttttcaacc gtgaggaggc cggggatctc cccggcagga agaccaagaa
    31081 gcgtgacata gccggaatca tcgccatatg tcatctcctc agcacttcgg agaacgggga
    31141 ttaccctgcc tgagcccacg acctttttta tcgcctcaac atccgactcg gggatccttg
    31201 ccatgactga aaccctcggg tccccgatga acccctgacc tgctgcaacg tgcggggtga
    31261 cgatgacagt gtctcccacg tcgctgatga ggtttgccac cagcaggctg atactgtggc
    31321 cgagtattcc catcgatgca attgccagta caccgatgat aatcccaaga atcgcaagaa
    31381 atgaacgtat ccagtggagt ttcaggttcc ttttcgccag gtccaggaag atcatgtgat
    31441 cttgccatca acaatccgga tggtgcggtg ggcaaaacct gcgatgttgg gatcgtgggt
    31501 caccatgatg atagtcttgc cccccttctc gtttaattcc ttcaggaggc tcataatggc
    31561 agaaccggtc tttgtgtcca ggttcccggt cggctcgtcg gcaaggagga tctcggggtc
    31621 gttgacgagt gcccgggcaa tcgctaccct ctgctgctgg cctcccgaga gctcgctcgg
    31681 cttgtgagaa tggaactgct cgtcgaggtc catcgacctg agaacctcca cgcagcgccc
    31741 cgtgcatgtg cgttcccctg tcttgaggat gagcggatac tggacattct caagcgctga
    31801 aaggaggggg atgaggttga actgctggaa gataaatccg atgtggtccc tccggagctt
    31861 cgtcagttcg tcgtcactca tggtgccgac atccctcccc ttgatgaaga gctggcctgc
    31921 ggtaggggta tcgagacacc ccatgaggtt caggagtgta gacttgcctg aacccgaggg
    31981 gcccatgatt gcgatgaagt ccccctgccg tatctccagg cttacatggt ccagggctac
    32041 gacgtctccc cccggaagtg gatagatctt tgtcacgtcc cggaactgga tgaccttctc
    32101 tccatccggc gacgagatca tctctttctc caggagaacc agacgatgcc tccgatcacc
    32161 gccgcacata ccaggatcac cgcgatcatc ataaggggca tcccttccgt ctcctgtgca
    32221 ccgggttttg tgaccgggat atcgattatc gtcatggtgg tgtagaggtt tccgtcattg
    32281 tccttgtaac tgacctctac ggggacctta tctgttcctt ctgccgtgaa tgtgacctca
    32341 aatgcggaga agtcatcggg ctggagggtc cccaccacgt atttcctgta agggtcaacc
    32401 gggaccaccg aatcaccggt gctgatgacc actgcattgg caacctcgag gccggcattg
    32461 ttcacgtcac ctgtgatgga gtacgttttt cctgacttct cgacctggat gttggagagg
    32521 atggggtcgg cccttgtctt gctcttccca atgccaatcg ggaattggat cacctcggca
    32581 tggggattga tgccgttcat gtagtccacg tggatctcca ggatggtggt ctgatggggt
    32641 gtgatcgaga aggtgacttt ttgagactgg tcgggggcga tcattccgat gaatatgctc
    32701 gatgggctcg cctcgattcc ctcccccatc ggatatacga tgacccccga gaccccgtta
    32761 tccctcgggt ttccaatcag cacttcgacc tgctcttttt tcccgctggt gaatacatcg
    32821 ggtttggaga ggatggagac cttcagcggt ttgtcctgga cctgaagttt cacggggtac
    32881 ctgagatatc cagcatcgcg gaagtccatt gagaagaccg gataataaat cccgtcaggc
    32941 accgtggtgg tcacggtgaa cgtgaaggtc atgcggttcc ccgcacccag gtacattgtc
    33001 gtgtcatagg cccgggactc tatcctgatc ttctcatcgt acatcgtgac cctgctgatt
    33061 gccacgctct gtgcgccgtt attaatcacc tccacggtga cagtcccggt atcgtcaacc
    33121 atgaacacct gggggtcgag cgtcacgttc gtcaccatga cctgcgcagc tgcagtctgc
    33181 tgctccaccg ctgatacagg gaggaagacg accatcactg caatgaaaaa gaggagcagt
    33241 tccgcacggt tcatctcatg caccacccgg cagggggatt ccgctcacca gcatgtagac
    33301 catgtagagg atatagactg ccaccggaat aatcacagtg atgcacgcat gtttcaacgt
    33361 gatgccgcgg gcttgtctga caccaaagat ccagatgttc gcgctccaga tcaggaacag
    33421 gatggagatt gcgattgaga cctgggtgaa ctccttcatt gccggatcga gcatcagctc
    33481 ctgcatggct ttctggattg cgacgggatc ctgaaggtcc cggacaacag ggaccgcgat
    33541 cctgggaagg tacgagaaga ggatcagggt ggagatgatg ctcccgatca cggttggcag
    33601 cagtccgtat cccgtgcttt caagggtccg ggtaaacttc cctgtcccct tgaaggccat
    33661 ggaaatcaca tggaagactc cggccattac cagccaccaa atgatgagga aaccgagaaa
    33721 cgcgcctgct gcacccatga tccccatgaa tgcccccatt cctgcacccg cacccatctc
    33781 cgcgaacata tctgcaaaga gagaggactg cgcatagcct gcaaccgcgg cgacgaccgc
    33841 ccctgcgagg gctattgccg ccggtatctt cagatccggt tccttccctt caagggttga
    33901 aaaaaaacgt tccgggtgga ccagaaggtc cagcaggtga atcatggtac aatcatctcg
    33961 cttgatcggt atatggcctt tccctccctc aatacttctc cctgctccat tcttgcccat
    34021 ccctcttccc acccgggtct gggatccccc gtttaaaaaa ttctccgggc aaacgggtgc
    34081 gtcccgtaag gctgggtcag cgtgcgctgc gtttcccacc ccgggtagta tctccacctg
    34141 catgtgagcg cgcatatcct aaagaaggct gaagcgtttc ctttgggtcc ccttctcctc
    34201 ctgaagtgcg tgctgaattc gggcaggaaa tctctttaga ttcttaaaat aagaccttcc
    34261 tgggctggat accagtcaaa ggcttctctt tcacatgaga ccctcgtgct gtcagaagaa
    34321 ctccttcctc cagaatatga tgatcccaac cgctgaaagg gcaagggata tcgcgaccgt
    34381 gacgaggaag gcatagggcg tatgctggta gggaagctcg acgttcattc cgtagatgct
    34441 ggccacaagg gtcggtatca tgagaatgat ggtcaccgtg gtgaggagct tcaacaccac
    34501 gtttaagtta ttggagatga cagatgcgaa cgcatccatc atgtccgaga gaatgctgct
    34561 gtagatattt gccatctcga tggcctgctt gttctcaatg atcaggtcct cgaagaggtc
    34621 gcgatcctcc tcgctcatct tcatgcagga ggtggtgttg aacttctcca tcatcagtgc
    34681 gttggagcga agcgacgtca cgaagaatac gagacttttt tccagcatca gcaggcggat
    34741 cagctgttcg ttcttcaggg ccttgtaaag agatctctcg acggttgtgg tcatcttgtt
    34801 gatctccttt aagtaccgca tgaagacgag cgtgctgcgc aggaagagga ggaggacaaa
    34861 acgggtctgc tgatctgtgg agaagtgttt caccttcccg gtgaaaaact cctggatgac
    34921 gtccgattca ttcagggaca cggtgatgag gacgttctgc gtgaagatga tcccgatggg
    34981 aagcgtggta aatggaatgt ctgcatcagg taattccctc cgcgggcttc gcaacaggat
    35041 gaggatgcac ccctcctctc gctcgatgcg ggcacgctcg tcgacgtcca ggggatcggt
    35101 gaggaaatca gaggggatat tgaaccagga gaccagctcc tcgatctcct tttgggtggg
    35161 atttacgacg ttgacccagg tcccgctctc gtacttgttg acccgctcca gcccatccgt
    35221 cccacccctg taaatggtta tcatggctgc ccacgtcccc cgggccgctg gaggaatcat
    35281 cggggtttgg gacagggaag acgaccccgc acgcattccc ccccttcacc cgttccaact
    35341 gtccgaatga tcctagatct ggtcgttttc ccggatacgc aaaatacctt tcgatcttct
    35401 gccccaaaat agccggaaaa gaggcttttt tccctggatt taggcgttgc ccttctccgc
    35461 cacggcaagt tcatgactca ggtcgcctga aagatctacc tccgtgaaac tcccctcggt
    35521 cgggaggaca acagtgaacc ttgacgtgac atccatctcc accgtcgcat tctcaaggac
    35581 aaaatccagt atgtggccac cggccgatcg atcttccgtt atatagtgga gatggaatcc
    35641 gggcacattc aggcccccga caagatcggg ggtccagaaa ccgacaaccg ttcctctcgt
    35701 ctcatggaat tcgaatatgg actggttggc tgcagcatcc acgagccggg cgttcggcgt
    35761 ctcctgccgt gggatgctcc gggccttgac gtatgggaac tctccgcgga tctgaattgc
    35821 atacatgtgg ttcctggagg gaaggaacgc ctccgcctgc ctggaaaaat cactgaaatt
    35881 ggcagcatac cttatgggga aggtgcaatc ctgggagaaa aaagtgacac tcccaaaggg
    35941 cgttgtcatg tcatcggtaa ccgcaacgac ctgaccgtca gcctttatct ggtagaagtc
    36001 cccgccgagc gcgatcatct ccccgtcgag gctgtcgaag gtggcaatgc cgaaatcacc
    36061 gtgttttttg acctcgccaa aggtcatggt gccctcacag gacccctgca ggagggcatc
    36121 gattgtggac acctggtaga tgacctcctg gtctatctca ttctcgggca tggccgcaag
    36181 ccagaacccg gtcgcaaacc cggctgctac gcagacagcg catactgcaa ctaccatccg
    36241 gtcctgcatt cagggtccgc ctccttctgc tgcgtctttt ttaatatctc tcatttcatt
    36301 gcactcccgt gcctcgcgta cctgttattg tttgccccga ttcataggga cacgctgtcc
    36361 gcattgatcg aatcagagtt cgccgatatc ctcgttccag agttcggggc aacgggtgat
    36421 aaactccttc atcatggaga tgcagtcctc cctgtgcagg tcgattacct gtaccccttt
    36481 tgcctccagg aggtccctgg caccgggaaa attttctgat tcgcccgcga caaccacggg
    36541 gatcccgaac tggactgccg cccccgcaca gagataacag ggcatcagcg ttgagtatag
    36601 cacacaattg cggaaactcc cgacccggcc ggcattctgg aggcaatcta tctccgcgtg
    36661 aaggatgggg ttgccctcct gcaccctgcg gttatgacca cggccaagga tctgcccatc
    36721 tctgaccagg acagacccga tagggatgcc tccttcggag aggccggccc tggcctcggc
    36781 caccgcacag tccatccagc ggtggtgcat tttgtctttc atgtattcca tcctttttta
    36841 ccctcctctc actctccccc tgattcccgg attgcctgac gggcggcgaa cacaagggta
    36901 atgcccggtt cctcccatct gaagatcttc cctggggtcg ttttccatga tgggattggg
    36961 actcctgtcc tgactaacgt ttccttttta ctacgcgcgg aactggcctt cacgttccca
    37021 tgcgaaaatc ctgtcccaca ggcagtcgcg gagagcccgg aacccctcca gcacatcccc
    37081 atccgccccg gaaaaagccg ccggatcggg gaatatctcc ctgatcaccc gccgggaagg
    37141 ggggagacgc gagggatcct cccactcccc gaggacaacg aggacgtcgt actcccggaa
    37201 ggggagtgaa gagagcccct tcgatgactg gcgggagata tcgactccct tctctgccat
    37261 taccctcacc gccgtgccgt tcagcactga tggcctctcc cctgcgctga acacctggct
    37321 ggtcgtgggg aggagtgccc tcgcaatccc ctcggccatc tgggaacgcc cggcattcct
    37381 ggagcagaca aagaggatcc tcatggtcat gcaaaagagg gggaataata agggaaggcc
    37441 ctcaacggca gcgccggatg cggaggactg cctcccggag gttctccatg gaggtggcat
    37501 aggagatacg ctcccatccc ggggtgtgga acgcggaccc gggagtcact gcaacgtgca
    37561 cctcctcgag ccagcggcgg gcaatctcca tgtcgtcccc ttccaccttc acgtacgcgt
    37621 aaaacgcacc atccgcaggg gcgacctcga agcccatcag gaggagctcc tggacaaggt
    37681 agtcccttct ccggttgaac tcctttctca tcacctcgac gcatgactgg tcgcaggaga
    37741 gggctgccac cccgccgtac atggcaaatg tggttgggtg gctgacggag tgctgctgga
    37801 tcttcgacat ctggcggatg atggggaggg gtgcgaccgc ataaccgagg cgccatcccg
    37861 tcattgcata ggccttcgag aagccgttga ttgtgatcgt ccgttcggcc atgtccccga
    37921 tcgacgcaag ggacctgggc accttcccat agaccagttt ctcgtatatc tcatccgaga
    37981 gcgcgaagag atcatagtcc gtgcacatgt ccgagatgag cttcatcgag gcgtcgtcga
    38041 ggaccgcccc ggatgggttc gagggggagt tgaccaccac catcttggtg cgtggcgtga
    38101 cccggtcaag cacgctctca ctcacctgga atgcctttgt atcgaggggg tgatgaacgg
    38161 gccttccgcc tgccacctgg acacacggct cgtacgagac ccaggaaggg tcgaggatga
    38221 tcacctcgtc tcctgggttc agaaccgcct ccattgcgtc gtagatggca tccttggcac
    38281 cacaggtgac cagcacctgc tcggggatgc aggaaaaccc gttctcccgg ttgatcttct
    38341 ccgagatggc gttcagcagc tccgggatcc cgttgctcgg agcgtaatgg gtctgaccgg
    38401 atttgagcgc ctccatgcag gcatccttaa tgtgctgggg ggtgtcaaaa tcgggttccc
    38461 cgattgacag gcttatcacc gggatgcctt tcttctccat ctgcctggca aggttcgata
    38521 tctcgatggt tgccgaggcc gcgatccctg ctaccttgcg ggagagctcc ctcatttgga
    38581 tcgctggacc atctttactg ccgactcgac tgcccggcgg gcatagtcaa tccgctcgtt
    38641 cgcctcgagc cgcgtcatcc caggtcccga gatgccaaga cttacgggct tgtcgaactc
    38701 gagcgagagg tcgatgatct tccgggccgc atgctgaacc acgatctcgt cgtgctgcgt
    38761 ggccccctcg atgacgcacc cgatggtcac cacggcgtcg accttccccc gcttcagcat
    38821 ccgcttgatg gccaggggca tgtcgtatgc cccgggcaca tacgtgcact cagtgacttc
    38881 ggcgccgaga aatgcggcgt gctcccgtcc ttccacttcc atcatgtagg tgatgtcccg
    38941 gttgaactcc g
//
LOCUS       DAOQYR010000014        35817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_1380_length_35817_cov_9.210307, whole genome shotgun sequence.
ACCESSION   DAOQYR010000014 DAOQYR010000000
VERSION     DAOQYR010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 35817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35817
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1380_length_35817_cov_9.210307"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1356..2618
                     /locus_tag="PKM87_03180"
     CDS             1356..2618
                     /locus_tag="PKM87_03180"
                     /inference="COORDINATES: protein motif:HMM:NF024420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HNQ29102.1"
                     /translation="MNEITIRDDIFSANFELFRQKLRLYNKGEEFKSFYEGIPEKWEF
                     YKRFVRIEGQERLDLKSWKKEDIGNGNIIKKVIYAIEIDSLKNSVYKIELKNNLLTWD
                     LRNGEKGRDHKSLYDALENPGITRIYETLLFNFYFDLIDDKTAFNQFIELAGKKYSFI
                     AYLFFLKNWMEYLPIAPETFDTAFKMLKISFKTSRRCSWDNYQTFISILRLIKEKIGI
                     ETGLTNIELVDAHSYCWMLVRIPGDEKIDIEKRSSPKRKPLKIDHNKSVKFPEVTPKR
                     GMVVSYEEYLKKYHKNLTIGRKAEIIVCNSERDRLKEAGRPDLAKDVTRVSDNPTLGY
                     DIDSFEIDETRIYIEVKAVRSFKDILSFYLSRNEWEKSQSMDNYYFYFVSFSKEEGEE
                     IEFIQGKELLSQYMMASEYFVKLPLPGK"
     gene            complement(2620..2796)
                     /locus_tag="PKM87_03185"
     CDS             complement(2620..2796)
                     /locus_tag="PKM87_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29103.1"
                     /translation="MSKEIEDHKKDNNFTDYEKSLKGKEKITIEFEREVIEKIILNIL
                     SSENGQQILKKCNI"
     gene            complement(2800..3240)
                     /locus_tag="PKM87_03190"
     CDS             complement(2800..3240)
                     /locus_tag="PKM87_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29104.1"
                     /translation="MENICEICGFKYPPEAHYCGKCGVDLTDKTELQPPANPKKESKQ
                     SKKRNATKDTRLNENVIKWCEIHAVMTWHEMTFPFFLNLVLLFAEGKKDLGFCDCAVC
                     NLGYRELQTTIINLTEKKKLKPSSREIVSKYHGADIAGMKIHFP"
     gene            complement(3473..4111)
                     /locus_tag="PKM87_03195"
     CDS             complement(3473..4111)
                     /locus_tag="PKM87_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2115 domain-containing protein"
                     /protein_id="HNQ29105.1"
                     /translation="MMYTEEIAGTCSRLASLKTRGELGHAIAGIVLSYSPKDLQRMRW
                     NFTGKIQDMPLELRNKLEETITGHLHGTYQALRLNNQQGTFSHMGEPLPKDVGAYWKM
                     VAAQCSTGDPEKVRLRFLKFLLSGFCMFVQELPGHPVGMPFPGGEKVKVIDGVYYCPV
                     REKANDVDSALCPFCPALQTPEIGYLKPPVGAGKHRKEEFLRQTFDRHHYNG"
     gene            complement(4127..4813)
                     /locus_tag="PKM87_03200"
     CDS             complement(4127..4813)
                     /locus_tag="PKM87_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ29106.1"
                     /translation="MAHHTGAILQRDGKTFGIVTRVPGGYVTPEALENLAAVARKYHV
                     PIVKLTSGQRFMLIGIPEKDLAAIYKDLGPLGERETAPCVKYVQTCLGTDVCKYGVQD
                     STEFGLELEKKYHGVTFPAKLKFGVSGCPRCCGESYIRDIGVIGTQRGWTVVFGGNSG
                     RRPSLGTVIAEDLSRDDALDLVHRLLEYYRNNANPKERTARFLERIGFEQLKSELLTL
                     LPYIPLEDVR"
     gene            complement(4829..5167)
                     /locus_tag="PKM87_03205"
     CDS             complement(4829..5167)
                     /locus_tag="PKM87_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNQ29107.1"
                     /translation="MKIVDATKTASSSNPHHVDARKLCEIPHAVVVMITLQPGESLKR
                     HMTPVDVIFYVLEGTGVVEIGDEKATAEKDTLIESPARIPHRWINESTSVFRVLVIKV
                     PKPTEETKLL"
     gene            complement(5224..5583)
                     /locus_tag="PKM87_03210"
     CDS             complement(5224..5583)
                     /locus_tag="PKM87_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ29108.1"
                     /translation="MQESCTVSQTVRYLSKRWTLLIILELYKGDEYTRRFSELRDALP
                     GITPKVLSERLKELEEEEIIIRNVDATAFPVRTEYTLTESGLEIIEIIRDIKRWALKW
                     KIENIACGSQDCKECVL"
     gene            5669..7285
                     /gene="hcp"
                     /locus_tag="PKM87_03215"
                     /gene_synonym="priS"
     CDS             5669..7285
                     /gene="hcp"
                     /locus_tag="PKM87_03215"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNQ29109.1"
                     /translation="MFCYQCEETVKGTGCIVKGVCGKDEEIAAYQDVLVYLCKGLSVR
                     NLAAMEKGEGNPDAGLFIADALFATLTNVNFNRDRFTAMINTAVAIRDALPKTAAPEP
                     DACTWKPKGEQDILGKASTVGTLASENEDVRSLRSTLLFGLKGVAAYYTHAAVLGRTD
                     AAITTFMQKALASMLKDLPVPEMVALVLECGKVGVSVLALLDEANTSAYGKPQITTVK
                     TGVGKHPGILITGHDLKDLEMLLEQSRNAGVDIYTHGEMLPAHAYPVFKKYPHLYGNY
                     GGSWPHQREEFEQFNGPVLFTTNCLVPPKESYKGRLFTTGLTGYPGVPHIEAAADGRK
                     DFSGIIAFAKTCQPPNALPGSGNDLITGCAHDAVLALAEKVVAAVKNGDIRRFIVMAG
                     CDGRQKEREYYTEFAKALPKDTVILTAGCAKYRYNSLDLGTISGIPRVLDAGQCNDCY
                     SLVVIAQALAKAFGVGINELPVSYNIAWYEQKAVLVLLALLSLGVKDITLGPKLPAFI
                     SPAVLNVLVKEFDLKPNTTVPADLARMVGA"
     gene            7341..7712
                     /locus_tag="PKM87_03220"
     CDS             7341..7712
                     /locus_tag="PKM87_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNQ29110.1"
                     /translation="MEFKPESTEKTDKPDKKRDELKGKVLVLGDLVNYQVGTVASRMV
                     VNRKAGSITLFSFDENEGLSEHTAPYDAVVTILDGECEVWVAGKTFQMKEGETIIFPA
                     NAPHALSAITRFKMMLIMIRD"
     gene            7715..8002
                     /locus_tag="PKM87_03225"
     CDS             7715..8002
                     /locus_tag="PKM87_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAC family transcription factor"
                     /protein_id="HNQ29111.1"
                     /translation="MVGDEKDGYYCSICGGIPPDKVKTKRVLIDGKETGIDHLDFIFE
                     NVKTLQLADGSAISGEIMKRVKEFNYVPTKKEAVYAAALLEEYRQWEKEHA"
     gene            7995..8240
                     /locus_tag="PKM87_03230"
     CDS             7995..8240
                     /locus_tag="PKM87_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29112.1"
                     /translation="MLDRCPGAASIRTPTLTIKKCPQCGEEIEIFSNDVSVKCSRCGF
                     TVYNDILSCVQWCKYARECVGEETYRKLTEKKTNTSQ"
     gene            8264..9121
                     /locus_tag="PKM87_03235"
     CDS             8264..9121
                     /locus_tag="PKM87_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ29113.1"
                     /translation="MKRKIIHIDEEKCTGCGQCIPDCPEGALQLIEGKARLVSDLFCD
                     GLGACIGTCPEGAITVIEREAGAYDEKAVMANVVPQGPAVITAHLEHLLGHGEQALYK
                     LAIEYLDENHIPVPKHRIPGSHPDHEMHGHPSFMGCPGSAARSIPRRYQTESRQQAGA
                     TSSELRQWPVQLKLLNPYAGYFDDADLLIAADCVPFAFADFHREFLKDKIVIIFCPKL
                     DSDIEGYITKLSEIFSRHPINSITILHMEVPCCSGAAYIVNQAMERSEKKIPVREFTI
                     TIEGKVIKD"
     gene            complement(9283..9367)
                     /locus_tag="PKM87_03240"
     tRNA            complement(9283..9367)
                     /locus_tag="PKM87_03240"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9329..9331),aa:Leu,seq:taa)
     gene            complement(9640..10269)
                     /locus_tag="PKM87_03245"
     CDS             complement(9640..10269)
                     /locus_tag="PKM87_03245"
                     /EC_number="2.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF040696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-S-isoprenylcysteine O-methyltransferase"
                     /protein_id="HNQ29114.1"
                     /translation="MLIPWSGPSTMTPPIFLSLYISGFLLAAAIRLWYTQEYRRKIKE
                     QHWKPALDTWLVYLPALGMFIIPGIYILTPYLAPADYTLPESTGWVGAGIFGAALLLL
                     WRSHADLGANFTPNAEVQQGQRLVTTGVYEYIRHPMYASHFLWAFAQPLLLWNWIAGF
                     GLLITFIPLYVVRVPVEEKMLAGRFGAAYEQYMERTGRIIPRFGRSGTP"
     gene            10484..13117
                     /locus_tag="PKM87_03250"
     CDS             10484..13117
                     /locus_tag="PKM87_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNQ29115.1"
                     /translation="MREPAVKKTLYWCEQCNVPLIGRSCGCGAQGKALPLLQPYDVRP
                     ALSADRELIHTLLIERFGPIPLPQIILLNKTGGVDRADLVIMHGDRFGWLTFDPVTRR
                     FSLDIAPEALPYLIPHVTQGIVRLGTDADLGPEGGRIGGKRFPLKKPAGEGTVIVTYK
                     NRFGTGVVKEGFIRVKEVIPVTPKTPPDPDWDTAIRQNQYHLKNLERNAVRTIKRHLH
                     DRPCVNVSFSGGKDSMAALLLARKAGVEKAFFLDTGIEFPETVAFVESLGVEVIRQAG
                     DFWKAAEKAGPPGKDHRWCCKLQKLHPLKIYLETTGPCLTVQGNRWYESWNRADLDET
                     SQNPANPLQLNISPIRNWRALEVFLYLWWQHAPINPLYEQGIERIGCMVCPAMLESEY
                     EILRTLHPGETTRWDAFLDRWAGKKGFPEEFRSWGLWRWKALPPKMRELCRSRGIPFN
                     EDFTLKTVPGWRTPGGEGRESTMKPEGERKDRESFAVEEIRKDFPILGEIIYLDNAAT
                     GFSPEPVVEAMIEFEHRYRANVGRGVHRYTRIATQRYWHAHEKVANLISGNDGTTVFS
                     KNTTEAINMVAHGLSWRPGDRVVTTILEHHSNLLPWRELSRMGVTVEIIGIAEDFQLD
                     LDAFRAALCEPVRLVAMTHASNVLGVVTPVAEIARMCRKNGALLVVDGAQSVPHLPVD
                     VRALDCDFLCFSGHKMLGPTGTGVLWMKEPLLEPSILGGGMVESVTADGYVPLGGYEK
                     YEAGTPNIAGGIGLGVAAEYLMANGMDRIHRHESALTTRLIDGLQGIDDVRVYAPRDP
                     DARIGVVSFTIKGMHPHEVAQQLDDQAEILVRSGYHCCQPLMESLGLPDGTVRASIAL
                     YTTEQEIDLLLAAVRAISRGR"
     gene            13482..14393
                     /gene="nadA"
                     /locus_tag="PKM87_03255"
     CDS             13482..14393
                     /gene="nadA"
                     /locus_tag="PKM87_03255"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNQ29116.1"
                     /translation="MAEDSELREEILTLKEERDAVILAHNYQIDAVQDIADFVGDSLE
                     LARAAASQKAPVIVFCGVDFMAETAAILSPEKTVLLPAAGACCPMAEMVTAGELAVLQ
                     EQYPDAAVVAYVNTTAEVKAVSDICCTSANAVKVVESLSEQQVIMVPDRNLARYVARF
                     TTKEILPWDGFCLVHERFTCKDIQRARSRHPGSEVLVHPECRPEVIDAADRVFSTSGM
                     IRHVCADPAGEFIIGTEIGILHQLSRRCPEKHCYPLSSRAICVNMKKTDLEKVHAALD
                     RMLPRVQVPERIAIPARRAIERMLALP"
     gene            complement(14405..15049)
                     /gene="thiE"
                     /locus_tag="PKM87_03260"
     CDS             complement(14405..15049)
                     /gene="thiE"
                     /locus_tag="PKM87_03260"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNQ29117.1"
                     /translation="MAFDLYVITDPAIARGMPHAGIARRVVEGGADAVQLRDKILPGR
                     DLLAVACEIREITWEAGVLFFVNDRIDIALASEADGVHIGQDDLPIRDARSISPEGFI
                     IGVSVGNVNEAVAAESAGADYLSLSPIFPTTSKDDAGPGHGIAELQAICASVTLPVIA
                     IGGITPRNAPEVIAAGAEGVAVISAVMAQDDITAAARHMKAIIKDSKEKVSKVR"
     gene            complement(15039..15848)
                     /gene="thiM"
                     /locus_tag="PKM87_03265"
     CDS             complement(15039..15848)
                     /gene="thiM"
                     /locus_tag="PKM87_03265"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HNQ29118.1"
                     /translation="MDSTVYGNLFALVRKGRPLVHHITNRVTINDCANITLCAGAAPV
                     MAEAPEEAAEMAGLAGALVLNIGTLTQAQVGAMLLAGERANELGIPVILDPVGAGATR
                     MRTETVLLLLDELDVAILKGNTGEIGVLSGLGGTVRGVDSGGVSGDPIEVARECARAT
                     GTVVAMTGEIDVVSDGRRTFLVANGTPMMGNLSGTGCMAASVTGAFAAISDDTTTAAV
                     AALAAFGLAGERAMEGCFGPYSFRMALFDAMYRLGAADLAAGAKVSVPDGL"
     gene            complement(15848..16705)
                     /gene="larE"
                     /locus_tag="PKM87_03270"
     CDS             complement(15848..16705)
                     /gene="larE"
                     /locus_tag="PKM87_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HNQ29119.1"
                     /translation="MTGTTIEERMGQLRRRISGCDQLMVSFSGGVDSGLLAALGKEIL
                     GDNLQAVFIDSPLVPRSAVSEAREMAGLLGIPLGIITVPFPGEIVSRNLPDRCYHCKK
                     SFSELLRAWADEQGISCIADGVTESDRSEYRPGTRAATEEGIIHPFLEARITKPEIRQ
                     LARERGLPFWDKPSSACLASRIPYGEALTRENLHMVEEAEAGLAEAGFAGSRVRNHGG
                     IARIEVPEADIPRLVAARETIVALVKEAGFSYVTLDLMGYRSGSMDEGSGSMDEVRGN
                     VAVHGGEGS"
     gene            17015..17716
                     /locus_tag="PKM87_03275"
     CDS             17015..17716
                     /locus_tag="PKM87_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HNQ29120.1"
                     /translation="MMLFGKEGQEHLQDATIFIAGAGGLGSPVSMYLAVLGVGTLIIA
                     DNDRVDQTNLNRQILYRDQDIGRRKAAVAGERLQELNPDITVRAVDATIDQNTVSSLT
                     RDADGIVDALDDFPTRYLLNSAALERGIPLFHGGISGFFGQATTILPGKTPCLRCIIP
                     RPPPAEVFPVAGVTAGFIGMIQVTEVCKYLLCRGDLLAGRLLLWDGMQGRIEEIPVET
                     DPDCEACAGKYTAIS"
     gene            17722..18000
                     /locus_tag="PKM87_03280"
     CDS             17722..18000
                     /locus_tag="PKM87_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNQ29121.1"
                     /translation="MKITVRFFARFGELLGNTREIEAREGISVIDLVKEAASGNKAGF
                     DTIFDEEGAFRDFVIVMRNKKRIRTQDAGSIPVGEGDEIAVFPPVAGG"
     gene            complement(18170..18820)
                     /locus_tag="PKM87_03285"
     CDS             complement(18170..18820)
                     /locus_tag="PKM87_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5591 domain-containing protein"
                     /protein_id="HNQ29122.1"
                     /translation="MGGQEKGDPGQGERTVLTGPPFYLPEFERSYRYIIDEYEVEPRE
                     IAIFIPCAVRKPYSTSPSHQLIRMIISQVLDPSRYHIIVFGTCGIVPAELEEMYPYAH
                     YRYMLGKCTDETIRQDFLRIETERVAGYLEKTRDAYHYRIGYCLGLFREALIRGSEQA
                     GVPIDLLLPSRERINKIIEEEDCSFQEGSLSMDEYLGEFCEELIRFRNSLDRASSI"
     gene            18982..19563
                     /locus_tag="PKM87_03290"
     CDS             18982..19563
                     /locus_tag="PKM87_03290"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNQ29123.1"
                     /translation="MSEMDQIGKISLGKNAFPFPMPVTLLGATVHGKPNFMALGWVSR
                     VNANPPMIGCGVGRHHYTSAGIKQNKTFSVNVPGRSMMAEADYCGIVSGETTDKAGLF
                     SVFYGELKTAPMISECPVCIECQLVQSVTYPTNEFFIGEIVGSYTEERYLTGGKPDMK
                     KIDPLLLSMPDNRYWSLGEYAGDAWSIGKSLKK"
     gene            complement(19700..20680)
                     /locus_tag="PKM87_03295"
     CDS             complement(19700..20680)
                     /locus_tag="PKM87_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNQ29124.1"
                     /translation="MLRAGKHRNIMVQAVAVDLGATRTRVAIIGEEGQIGERREARTP
                     AKESDPSRLAGFLSSLISGVRAAHGEESLSGIGLSVAGPVDITRGVLVNPPNMAFRDV
                     PLREELSGIFHCPVRMVNDCHAGVLGEMHYGKGQGKQDVVYVTISTGIGGGVVSRGRL
                     LLGRKGNAAEIGHFLVDNTYDLPCSCGHRGHWEGYASGRFLPWFFAEWCRFHAKPHWG
                     PDAAEEIFASARQGDDDVLRFVRELSRINARGISDVIVAYDPEIIILDGAVMQSNADL
                     LLPDMREFIDRFLPLPEIVLSELGGDAPLLGAAVIAGGYETGYGDFRSAG"
     gene            20740..21744
                     /locus_tag="PKM87_03300"
     CDS             20740..21744
                     /locus_tag="PKM87_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HNQ29125.1"
                     /translation="MDLKGWVELDGKLISFRELETLLRTDPRTVARFGGEFLLSWDGC
                     IARDHLGIIPGPCPAGTISCEGREIGKVCPDPPAMSLEEAIVTAVRLRSTEGICALSG
                     GVDSSLVAFLAQRPCIAIGLAGSHDLARARLAAEAMQLTCTFIEVTSDDIREGLHATI
                     RVIPEPTPVNTAIAITQYLITRSAGELGHERVLTGQGADELFGGYARYRTGIDPAGRL
                     ARDFAGLAAQGLRDQSVAALNGTYLSQPFLDIRVVRAARSLPASGMVRDGVGKWPLRE
                     VAARHIPREIAWHGKKAMQYGTGIAKVLQGFARDKGYKRSLQRYLDQIRTSGEARNGI
                     "
     gene            21734..22003
                     /gene="gatC"
                     /locus_tag="PKM87_03305"
     CDS             21734..22003
                     /gene="gatC"
                     /locus_tag="PKM87_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNQ29126.1"
                     /translation="MVSEKDVAHIAELADIGIEKEELAEFTGRFNEILDYFDILDQVE
                     QGSRGGTDLVNIFRDDIVEDSLPVEEVLANTREKEDGFFRAPRVM"
     gene            22004..23302
                     /gene="gatA"
                     /locus_tag="PKM87_03310"
     CDS             22004..23302
                     /gene="gatA"
                     /locus_tag="PKM87_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNQ29127.1"
                     /translation="METLTFTRDDRYNAFITMLDKADTGQGKLSGIGIAIKDNISTRG
                     IQTTCASRTLEGYLPPYDAHVVTLVKKAGAGIVGKTNMDEFGMGTTTENSAFGPTKNP
                     ADTSRVPGGSSGGSAAAIASGMVQMALGTDTGGSIRCPAAFCGIVGHKPTYGRVSRYG
                     LVAYANSLEQIGPMARTVSDISLLMEVLAAYDPRDSTSVNCPYAHIPDPDVKGLKIGV
                     PEEYFGEGVDPRVASVVWDGISLLERKGADIITCRMPSMAYALAAYYVTCTSEASSNL
                     ARFDGVRYGPPADTRQTWHESFQERRRDGFGTEVKRRIMLGTFALSSGYYGKYYAKAQ
                     AARQQVRNDFLRLFREVDVIAGPTMPTVAFRIGEKTDPLSMYLSDILTVPANLAGIPA
                     ISVPCGTSEGLPVGLQIMGPHFRDERVIDVAYAFEQGVSR"
     gene            23341..24834
                     /gene="gatB"
                     /locus_tag="PKM87_03315"
     CDS             23341..24834
                     /gene="gatB"
                     /locus_tag="PKM87_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNQ29128.1"
                     /translation="MDFSDDEVIIGLEIHCQLDTLTKLFCGCSTDYRDDGPNTHVCPI
                     CLGLPGSLPRVNRKAIEYALRVAKALNCTIVEESEFARKNYFYPDLNKGFQITQYDRP
                     LAVKGYLDIEGDDGEKRVRITRVHVEEDPGRLVHKGGADRAKYTLVDYNRAGIPLIEI
                     VTEPDIRSPKEARKFLNKLRATLEYLGVFDSEKEGSLRVDANISIQPRGWFDREGKGK
                     ADRGRAEVKNISSYKGVEKALTFEVTRQKNALRRGQRLGTETRHFVETRGVTTSSRSK
                     EMEQEYRYFPEPDLRPLRVCAWADEIVLPELPDARRARFLTQYGCSPIHARTLTGDLR
                     VAEFYEQVAGIDPVLTATWVADTLLGELNYRDMSVGRVSPDWFGELIVLVKEGSVTDK
                     GGVEVLRSLLDQIRESGSCETPGVCVGRLGLRVLERTAETGSDDDPVVKAAREVVAEQ
                     PQAVADFAAGKKEAFNFLVGQVMKKTRGRAKPADVNQVLSAILGKEG"
     gene            24840..27677
                     /locus_tag="PKM87_03320"
     CDS             24840..27677
                     /locus_tag="PKM87_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HNQ29129.1"
                     /translation="MHLIVAEKNISAHRIASILAGKTKVATDKDGGVPVYRFGDTAVI
                     GLRGHVVEIDFEEGYSNWRSTERTPRSLIDARTIKVPTEKKIVHILQKLAKKSSLITI
                     ATDFDTEGELIGKEAYELIRQVSREVPVRRARFSAITPDEIRQAFSETTEIDFDLASA
                     GEARQVIDLMWGASLTRFISLAARRGGSNILSVGRVQTPTLAMIVDREKEIEEFVSQQ
                     YWQLSLMTEKDGEEIEARHATPRFCDKAEAVRARDRTLPPLIVTEVKEGAKIERAPVP
                     LDTTGFIVAAARLGFSAAGAMRIAEDLYMNGYISYPRTDNTVYPPTLDLGGVLKALSA
                     TEFEGEVAWVTTHRRPAPTRGKKTSTDHPPIHPASAATRHSLGEERWKIYELVVRRFL
                     ATLSPDARWKTMKVNFRAGEEPYTATGGQLTEPGWRKVYPYSTATEYILPPMKVGEQL
                     PIREVTLEEKETQPPARFTQSKLIQKMEELGLGTKSTRHEVIQKLLSRKYIEGNPLRP
                     TLVGRAVTESLERHANTITRPAMTQTLEEHMQQIKEQHRSRDDVVAESRGMLHSVFDQ
                     LEPNEQVVGQEIMEQTAEELTLGPCPVCSHDIRIRHMRNQTQFIGCTHYPDCTFNIGL
                     PQAMWGFAVRTDDTCPIHQLHFVRLVRKGSRPWEIGCPLCHQIASNKEALFLMASMTP
                     EIHERMIAAHLYTVTDLAGRDPAQLAPLLGISGEKAGDLVREAEGVLGLLRRRSDCRK
                     LIRRMLVPRKGRSPASVIKKLQEAGINDIASLGTADPALLKRVGIADKEAESILHEAK
                     MVENERFLKQAGIPAASMKKYFSAGISSPEDFLSSHPVFISDTTGISLDTVQKHVSLV
                     AAAKGRPAPVRYTKVQVAKGREELTSLPGFSEEDLRRLWRAGIINGLLLLESDPESLG
                     ERTGISPARLRRYQAVLQERAKKAEPDIIVV"
     gene            27687..28373
                     /locus_tag="PKM87_03325"
     CDS             27687..28373
                     /locus_tag="PKM87_03325"
                     /EC_number="2.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerol geranylgeranyltransferase"
                     /protein_id="HNQ29130.1"
                     /translation="MHTRWKNWVHVTKLDPDKMLPAGAIAEIVSSGTDALMLSGTLNV
                     TAENMAALAKEARASGLPLVVEPAGPEAVLWDGVDLVFVPSVLNSPEVQWIVGKHRSW
                     IQRQQVEWEKVVPEAYIVLNPDSAVGKLTKATCTLTGKEVASFASLADHYFHFPIVYI
                     EYSGIYGDPGVVRETAAAVENAVIYYGGGINSAAKAAEMARYADTIVVGNAVYDQGIG
                     VLKETVRAIQ"
     gene            28386..29135
                     /locus_tag="PKM87_03330"
     CDS             28386..29135
                     /locus_tag="PKM87_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNQ29131.1"
                     /translation="MPEIEIALVSPLYEGNVGFAARVMKNFGFTRLVLIDPCEIGDEA
                     FMRAAHASDVLENAERLSFEEVCARSSIVIATTGEVSKSVCNPMRMPYFSPNEIRELI
                     ADIDGRISILFGRENWGLNNEEIQKSDIVCTIPSSKEYPILNLSHAVGIVCYELAHIR
                     RGEYLLATREEMEYLYAHIDSYLTQIDHPDFKRVPTMLLIRRVLGRAMLTSREATTLH
                     GLMRRSEWKINPAAFERIKGNSSGDDDQSSG"
     gene            29536..31437
                     /locus_tag="PKM87_03335"
     CDS             29536..31437
                     /locus_tag="PKM87_03335"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNQ29132.1"
                     /translation="MQEFAGEGNRDYTCSYGTKYLLKQISALRKQIPGVIAAHDIEHI
                     HQMRVATRRLRSALPLFPLCLPQKKIQRWRRDLRNTARSLGKARDLDVQISFLTEFIA
                     DLKAGRPMENRVLLPSVVPETPPPVPFNPKWYTLSISYRKLVTTFQSALSRAWNYFSH
                     TAVEGDLAGGDCFSCQADLIPGLEYLLFRKNKERSDYQSEVKKTIWQMEKAGILGAME
                     KYLQRHRPVERASLQVGRETYATAFTSIALRIEEVLSFSESLADPGKIREHHALRIAV
                     KRLRYTLEVWRDLFDGTINKEIGALKEFQDLLGDLHDCDVWTEYLPEFLKGEEERCRV
                     FFGNDTHFKSLVPGIEALARERRERRGQLHEITLTTWGELESRQFWYGLMEKFILPLA
                     LREDLPVRIGILGNISGDTTALRRVLAECYSRDASLVLNTGTSLGSPRASEKTVDLLR
                     EEGVVSIATANDLQILDGSSSVMTDGDSGVIQSRKRQSKKTRKFFRTLPTSLRVFVAG
                     KSILITGDSSETAIWSFDEKTTTERLCEIANEARASVIVSGSSRMPFTIRACDVLFVH
                     PGSVGREGVPFSTFSILEVGLGGSMAVTRHEIVIDPVGPGSDSTLRQPESSSPSLGEE
                     GQNHQPERC"
     gene            complement(31535..34351)
                     /locus_tag="PKM87_03340"
     CDS             complement(31535..34351)
                     /locus_tag="PKM87_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNQ29133.1"
                     /translation="MADKIQQPPKWYSVPPEAIAQQLGVDPGKGLSADEVHQRQQKYG
                     PNILKARRKEPGWYAFLRQYRDFMQIILVGAALLSLVITREAGTTVVLLGLTVFNAVL
                     GLRGEAKAEASLAALEKMMKNITRVRRDGRAVEIESEGLVPGDIVLMEAGNRVPADGR
                     LFVAANLEIEEAALTGESVASPKNTESIPRPEVPLGDRHNMAFMNTSVTRGRGEMIVT
                     ATGMETEMGHIADLLNRTRSDKTPLQKQLDRLTIIIAGMAGIAFVLMVILGLNRGDSF
                     DTIFVAGIALAIAAIPTGLPAVVTTMYSMGTRALASLGAIVKRLPSVETLGSVSAICS
                     DKTGTLTLNKMTAREFSIPGQNRFRVTGEGYGTQGTMELSTGMTEKQPWQKGTLSYTT
                     EGKLLSAGGARIDLDPILLPMALCADARLDNGTLIGDPTEGALIVLAEKGGISVDGAR
                     DMYPRIAEVPFDSDYKFMATFHAMTNEDGKPVVRCFVKGAPDVLIARSSSYWMPGGGV
                     LPAIEENRPLALKENDRMAEGGERVMVVARRDFDPKTFDPNANLIEQVRDLTLLAMVG
                     IVDPPRPEARDAIAKCHSAGIQVRMITGDHAVTAAAIGRELGIVGKALTGAEFAAMSD
                     EQLLKELSDIGVVARVAPEDKIRLVDLLQRQQNIVAMTGDGVNDAPALKKADIGVAMG
                     ITGTEVSKGAAVMILTDDNFATIVKAVEFGRAIYGNLFNFVRFQMTSLVGYIAAYLGA
                     AIFVITGGVPFAPFVVLWVNFFVQVPVAIALGFDKPEPGLMEKKPRPLKQPILNRPQW
                     LRIGFIGVLTAAGTLSLAAVAESTGFTLAATMAFVSFSLFCVTRGLACRSETQSALNR
                     DVLHDRNQSMLFGLALLLIFLPTQLDFLQRGLGLTSLEGSQWLLCIAAAIALLLVDEI
                     VKVFLRSRHMKDVIPPQGAVPARV"
     gene            complement(34367..35266)
                     /gene="ppk2"
                     /locus_tag="PKM87_03345"
     CDS             complement(34367..35266)
                     /gene="ppk2"
                     /locus_tag="PKM87_03345"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HNQ29134.1"
                     /translation="MAKKKGKKKKLRKAERQQQDEVQSGIPAVSDRPAPGATPESKPK
                     MSRKEFEKELVNLQVELVKMQEWVKATGAKVCVLFEGRDAAGKGGIIKRITERTSPRV
                     FRLVALPTPTEREKSQMYVQRYIPHLPAAGEVVLFDRSWYNRAGVERVMGFATKEQVE
                     NFLRWVPPVEHAMIASGIILVKYWLEETMEDQTQRFEERITDYRKIWKLSPMDLESHR
                     RWYDYSRARDAMFAATDTPESPWFVVDSHDQRRGRLNCIAHLLSLIPYKEIPREKVKL
                     PKRQPKGDYVEPDHPFRWIPEKY"
BASE COUNT         8073 a        10129 c        10135 g         7480 t
ORIGIN      
        1 atccctcctg caggtcccgc caccgcctcc cgcctgccgg cccgcgacag ctcgccccca
       61 gcgagccgta accggggccg tcttcccacc cactcggcgt tctgtgattt ctgtccgttc
      121 gtcacccctc gacttcgggc tgatgccctc agttcccggg gttttcctcg ctggtctccg
      181 gttctctgga tatagctgac ggtcgaccgt gctcatctcc actccctccg gtccgttccg
      241 ctcgcaccgt ctccctggtc tctcttcctg aactgttgat acgttgacgc ccatgatttc
      301 gtcgcaggct cctcatcacc ggctgcacat cccgctcctc gcggaccgct acacggaccg
      361 ctcgtcgctg ttacataaac tcctttgacg ttcctcagaa tattccagaa aaggtaagtg
      421 cccgaagggc atgcttcatg atcactgcct ctcccttggg agaggaggag gccatcccat
      481 cggggcgagg agcgaagggg gtagcagtgc ccccctggaa gcgatgagag gccgggggag
      541 tggggacttg cgacgcatca gcggagccgg actggccaca gcggcccgag cgagcgccgg
      601 ttaccgagtg catcagcacg aggtcgagcg ctgcgagtca ctgagcgtag gtggcgtacg
      661 gaacacagcg acaagcacga tgcgaagcat ggtgtgagga gcggggggga gcaggcgttg
      721 cgatgtgagc gaagcgagtg agcatggccg tcatcaggac caggagcgtt ctgaaaaaca
      781 gacacacgat gtgagtgaag ggaggagaga gcaccggacg aacggaggag cggaacgacg
      841 accgttgcga gcatggtgtg gcgtctaaat attacttgta ggaaattact tcgactgggg
      901 tacaaatgta tttacaatca agattccgtt ggaagtaaaa ggtccccgaa cccgattgat
      961 tagggagtaa ttctgcaata aagacacttc cttcagtatt ttcacaccaa ccggaattgg
     1021 aattacactc aattttaata tttaagttat atttactgaa gttttcatta ttttttgcaa
     1081 caacggtgac cgaataatta cgatttaagg gatctattgg tatagaattg taatattttg
     1141 gttcgagttt gttatcgaca atacaattga cggaaacgtt gtttcgctca tcgacggtag
     1201 taatgcaaca agcacccaat gcaaaaatta tcatgaggat cattagcatc agaaggtgca
     1261 gtctcataat tcacagatta ttcattccta ttaaaaagct ctctccgaaa aacgatgctg
     1321 cggttcttat atttataaaa aaatgaactc ttaatatgaa cgaaattaca ataagagacg
     1381 atatttttag tgcaaatttc gagttattca gacaaaaact tcgattatat aacaagggag
     1441 aagaattcaa atcattttac gaaggtattc cggaaaaatg ggaattctat aagagatttg
     1501 tccgtattga aggacaagaa cgattggacc tgaaatcatg gaaaaaggaa gatattggta
     1561 atggtaatat tattaaaaaa gtaatttacg cgatagaaat tgattctctg aaaaattctg
     1621 tttataaaat tgaattaaag aataatctcc tcacctggga tttaagaaat ggagagaaag
     1681 gcagagacca taaatctctc tatgatgcat tggaaaatcc tggaataacc cggatatatg
     1741 aaacattact ttttaatttt tattttgacc ttattgatga taagactgca tttaatcaat
     1801 ttattgagtt agcggggaaa aaatactcct ttattgccta tcttttcttt ttaaagaatt
     1861 ggatggagta tctaccaata gcacctgaga catttgatac cgcattcaaa atgttgaaaa
     1921 tatcttttaa aacaagccga agatgttcct gggataacta tcaaactttc atttccattt
     1981 tacgtctgat taaggaaaag attggaattg agaccggatt aaccaatatc gaattagtag
     2041 atgctcattc atattgctgg atgcttgtaa ggataccggg tgatgaaaaa attgatattg
     2101 aaaagaggtc ctcgccaaag agaaaacctt taaagattga tcataacaaa tcggttaaat
     2161 ttcctgaggt aaccccaaaa aggggaatgg tggtatccta tgaggagtat ctgaaaaaat
     2221 atcataagaa tctcacaatt gggcgaaaag ctgaaattat tgtttgtaat tcggaacgtg
     2281 atagattaaa agaagcagga aggccagatt tagcgaagga tgtaactaga gtgtctgata
     2341 atcccactct tggatatgat attgactcgt ttgaaattga tgagaccagg atttatattg
     2401 aagtaaaagc agtaaggagt ttcaaagata ttttatcgtt ttacctctcg agaaatgaat
     2461 gggaaaagag ccagtcaatg gataattatt atttttattt tgttagtttt tcaaaagagg
     2521 aaggcgagga gattgaattc attcaaggta aggagttatt gtcccaatat atgatggcct
     2581 ccgaatattt tgtgaagttg cctctaccag gaaaataaat caaatattac atttttttag
     2641 tatttgctga ccattctcgc tcgaaagaat attcaggatt attttttcaa taacttctct
     2701 ttcaaattca attgtaattt tttccttccc tttcaaagac ttttcataat cggtgaagtt
     2761 attgtctttt ttatgatctt caatttcctt gctcattaac tatggaaaat ggattttcat
     2821 accggcaata tctgcgccgt gatacttgct aacgatttct ctgctcgatg gttttagttt
     2881 cttcttttcc gtcaggttga tgatcgtcgt ctgaagttca cgatatccca aattgcagac
     2941 tgcacaatca caaaacccta gatccttctt accttcagca aataataaaa caagattgag
     3001 gaagaaaggg aatgtcattt catgccacgt catcactgca tgaatttcac accacttgat
     3061 aacattctca tttaatcgag tatctttagt ggcattcctt ttcttcgatt gttttgattc
     3121 cttcttagga tttgctggtg gttgcaattc ggttttatcc gttaggtcaa ctccacattt
     3181 cccacaataa tgagcttctg gcggatattt gaatccgcaa atttcacaga tattttccat
     3241 gattcagaga aggtcataca gattaaaatt gatctgccgc acggtacatt ccctataaaa
     3301 accaccttac acttttccac ccgtattcct gcgcgagcat aaaaataact gatttagggc
     3361 tccgttgaca acccggctgg ggcttgggat ttgaatataa tccataaatc acccattttt
     3421 gcacttttgc cagatcgttc agtacaatac ccgtaatttc cgtccaaatc ttttacccgt
     3481 tgtagtgatg ccggtcaaag gtctggcgga ggaactcctc ttttcggtgc ttccctgcac
     3541 ccactggcgg cttcaggtat ccgatctccg gggtctggag ggccgggcag aaggggcaga
     3601 gagcggaatc gacatcgttt gccttctccc tgaccgggca gtaatagacc ccatcgatga
     3661 ctttgacctt ttcacctccc ggaaacggca taccgactgg atggccgggg agttcctgca
     3721 cgaacatgca aaagccggag agcaagaatt tcaggaagcg taacctgacc ttttcgggat
     3781 cgccggtgga acactgggct gcgaccatct tccagtatgc acctacatcc ttgggtaatg
     3841 gttcacccat gtgggagaat gtcccctgct ggttattgag ccggagtgcc tggtaggtcc
     3901 cgtgaaggtg cccggtgatc gtctcttcca gtttgtttcg cagttccagt ggcatgtctt
     3961 ggatcttccc ggtaaaattc cagcgcatcc gctggaggtc cttgggagag tacgagagca
     4021 cgatccctgc tattgcatgc ccaagctcac cacgggtctt cagtgatgcg agtctcgagc
     4081 aggttcccgc aatttcttcg gtatacatca tgacagcctc tgtcccctac cgtacatcct
     4141 caagggggat atacggaagg agggtgagaa gttctgattt caactgttca aatccgatcc
     4201 gctccaggaa ccgggctgtc ctctcctttg gattggcatt gttccggtaa tattcaagca
     4261 gacggtggac gaggtcgaga gcgtcgtccc tggagagatc ctcggcaatc acggtcccaa
     4321 gagagggtct ccgcccgctg tttccaccga atacgacagt ccaccctctt tgggtaccga
     4381 tgaccccgat atcccggatg taactttcgc cacaacaacg ggggcagccg gacaccccga
     4441 acttgagctt tgccgggaag gtgacgccat ggtacttctt ttcgagttcg agcccgaact
     4501 cggtggagtc ctggacaccg tacttgcaga catccgtccc caggcaggtc tgaacatact
     4561 tgacacaggg ggcggtctct ctctcgccta atggcccaag atccttatag attgccgcaa
     4621 ggtccttttc agggatcccg atcagcatga accgctggcc tgaggtgagc ttcactatcg
     4681 gcacatggta tttccgggca acggcggcaa gattctcaag cgcctcggga gtgacgtacc
     4741 ctcccgggac ccgggtcacg atcccaaagg tctttccgtc gcgctggagg atagcaccgg
     4801 tatgatgagc catggtaatc tccttccctc acaacagctt ggtctcttcg gttggtttcg
     4861 gaaccttgat gaccagcacc cggaacaccg atgtgctctc attgatccat cggtggggaa
     4921 ttctggcggg gctctcgatc agggtatcct tctcagcggt cgccttctca tcgccaatct
     4981 caaccacacc ggtcccttcc aggacgtaga agattacatc gaccggcgtc atatgccgtt
     5041 tcagcgattc accgggctgg agtgtgatca tgaccacgac ggcatggggg atctcgcaga
     5101 gctttcttgc gtccacatgg tgggggttag atgagctggc ggttttggtt gcatcaacga
     5161 tcttcataac aaacacatct cttcgctttc tctgttcttt ttgcgatcat acctggacag
     5221 gattcagagg acacactctt tacagtcctg gcttccgcag gcaatgtttt caatcttcca
     5281 cttcagggcc caccgcttga tgtcacggat gatctcgatg atctcaaggc cgctctcggt
     5341 caaggtatac tcagtcctga cagggaatgc ggtcgcatcc acgttccgta tgatgatctc
     5401 ctcctcctcg agctctttca accgttcgga cagaaccttg ggagtgatgc cgggaagggc
     5461 gtctcggagc tctgagaacc tcctggtata ctcatcgcct ttataaagtt ccaggatgat
     5521 cagcagtgtc caccgtttgg aaagataccg gaccgtctgg ctgaccgtgc aggattcctg
     5581 catggtatct ttatggatac ttctggatat ttatacttta gtatgaaata gatattttgt
     5641 atcaaaaata aatcatagga gtctgatgat gttctgctac cagtgtgaag agaccgtgaa
     5701 aggaaccggt tgtatcgtga aaggtgtctg cgggaaggat gaggaaatcg cagcatacca
     5761 ggatgtcctc gtgtacctct gcaagggatt gtccgtgcgg aaccttgcgg cgatggagaa
     5821 gggagaaggg aatcctgatg ccggcctgtt catcgcggac gcactctttg ccaccttgac
     5881 caatgtcaac tttaacagag accgtttcac ggcaatgatc aataccgctg ttgcgatccg
     5941 tgacgcactt ccgaaaaccg ctgctccaga accggatgct tgcacctgga agccgaaggg
     6001 tgagcaggac atccttggga aagcatccac agttggaacc ctcgcgagcg aaaacgagga
     6061 tgttcgctct ctcagatcaa cactcctgtt cggcctcaag ggtgtagctg cctattatac
     6121 ccatgcggca gtgctcggca ggaccgatgc ggcgatcacc actttcatgc agaaagcgct
     6181 cgcctccatg ctcaaggatc tcccggtccc cgaaatggta gcactcgttc tggaatgcgg
     6241 gaaggttggg gtcagtgtcc ttgccctgct cgacgaggca aacacttcag cgtacggaaa
     6301 gccgcagatc acgactgtga agaccggagt gggaaaacac ccgggcatcc tgatcaccgg
     6361 ccatgacctg aaagaccttg agatgctgct ggagcagtcg aggaacgctg gggtggatat
     6421 ctatactcat ggcgagatgc taccagcaca cgcgtatccc gtgttcaaaa aataccctca
     6481 cctttacggt aattatggcg gttcatggcc acaccagcgg gaggagttcg agcagttcaa
     6541 tgggccggtc ctgttcacga ccaactgcct cgtcccgccg aaagaaagct acaagggccg
     6601 gctctttacg accgggctga ccggctatcc aggcgttccg catatcgaag cggcagcaga
     6661 cgggaggaag gacttctccg ggatcatcgc cttcgccaaa acctgccagc ccccgaacgc
     6721 actgcccggg agtgggaatg atctcatcac cggatgcgcc cacgatgccg tcctcgcact
     6781 tgcggagaag gtggtagcgg cggtcaagaa cggggacatc aggaggttca tcgtcatggc
     6841 cgggtgcgat ggcaggcaga aggaacggga atactacacg gagtttgcga aagccctccc
     6901 gaaggacacg gtcatcctca ccgcgggctg cgccaagtac cgctacaatt cgctcgatct
     6961 cggtacaatt agcggcatcc cccgggtcct cgatgccggc cagtgcaatg actgctactc
     7021 gctcgtagtg atcgcccagg cactcgcgaa ggcattcggt gtcgggatca acgaactgcc
     7081 ggtatcgtat aacattgcgt ggtacgagca gaaggccgtg cttgtcctcc tcgccctgct
     7141 ctccctcggg gtaaaagaca tcacgctcgg cccgaagctc ccggcattca tatcacccgc
     7201 ggtgctgaac gtgctcgtca aggagttcga cctcaagccg aacaccacgg tgccggccga
     7261 tctcgcccgc atggttgggg cctgaaaccc ctatgatttt tatcgcagga agatgatgat
     7321 ttcgagaaga gcgaattgat atggagttca aaccagaatc cacggaaaag accgataagc
     7381 cggacaagaa acgggacgaa ctcaagggga aggtactagt cctcggggac ctcgtgaact
     7441 accaggtcgg gacggtcgcg agccggatgg ttgtgaaccg gaaagcgggc agcatcacgc
     7501 tgttttcatt cgatgagaat gaagggctgt ccgagcatac cgccccgtac gatgccgtgg
     7561 tcaccattct tgacggagaa tgcgaggtct gggttgcagg aaaaaccttc cagatgaaag
     7621 aaggtgaaac aattatcttc ccggccaacg ccccgcatgc actctcggca atcaccaggt
     7681 tcaagatgat gctgatcatg atcagggatt gaccatggta ggcgatgaga aagacgggta
     7741 ttactgctcc atatgtggag ggatccctcc ggacaaagtc aagacaaaac gggtcctgat
     7801 agatggaaag gagaccggca tcgatcatct cgatttcatt ttcgagaacg tgaaaacgtt
     7861 gcagctggcc gatggttctg ccatttccgg agagatcatg aaacgggtca aggaattcaa
     7921 ctacgtgccg acgaagaagg aggccgtgta tgcagcggcg ctcctggagg aataccgcca
     7981 gtgggagaag gagcatgctt gaccgctgtc ccggagcggc aagcatccgg acaccgaccc
     8041 tgacgataaa aaagtgtccc cagtgcggcg aggagatcga gatcttctca aacgatgttt
     8101 ccgtgaagtg cagcaggtgc ggcttcaccg tctacaacga tattctctcc tgtgtccaat
     8161 ggtgtaaata tgccagggaa tgtgttgggg aggagacgta tcggaaactg acggaaaaga
     8221 agacgaatac atcgcagtga agaatttccc tgaagagaat gccatgaaaa gaaagatcat
     8281 tcatattgat gaagaaaagt gcacgggatg cggccagtgc atccccgatt gtcctgaggg
     8341 cgcattgcag ctgattgagg gaaaagcacg cctggtaagc gacctcttct gcgacgggct
     8401 cggtgcctgt atcggcacat gccctgaagg agcgatcacg gtcattgaac gggaagccgg
     8461 ggcctatgat gagaaggctg tcatggccaa cgttgttccg cagggacccg cggtgatcac
     8521 cgcccatctt gaacacctcc tcgggcatgg agagcaggcc ctctataagc ttgcaataga
     8581 ataccttgac gagaaccaca ttcccgttcc caagcaccgt atccctgggt ctcaccctga
     8641 tcacgagatg catggccatc cttcctttat gggctgcccc ggatctgcag cacggagtat
     8701 tccccgaaga taccaaactg aatcccggca gcaggcggga gcaaccagtt cagagctgcg
     8761 tcagtggccg gtgcagctca aactccttaa cccatatgcc ggatacttcg acgatgccga
     8821 tctgctcatt gctgccgatt gtgtaccttt cgctttcgca gattttcacc gtgaattcct
     8881 gaaggataaa atcgtgatca tattctgccc gaaactggat tcggatattg agggctacat
     8941 tacaaaactg tccgagatat tctcccgtca cccaatcaac tcaattacca tcctccatat
     9001 ggaagttccg tgctgcagtg gtgcagccta tatcgtgaat caggcaatgg agcgatcaga
     9061 gaaaaaaatt ccggtaaggg agttcacgat cacaatagag gggaaggtga tcaaagattg
     9121 atggcgatta gtcatctgcc gcacggtcat tccactataa aaactccctt acactttttc
     9181 acccgcattt ctgcgcgagc agaaaaataa ctgatttacg ggctccgttg ttaacccggc
     9241 tggggcttca gaactcaatg ttcaaatacc ctaatgaaaa catgcgaggg gtgggattcg
     9301 aacccacgaa cacctccgtg acgggatctt aagtcccgct cttttggcct ggcttagata
     9361 ccctcgctca aaatgtgatt ctgtaaataa taatcgtgaa gaatcataag acagtcgatc
     9421 aatatttttt actcaaatga gtaatatcca aacatgatcc gacgccatta aatggcagca
     9481 ggtcttaagt ccggctcttt tggcctggct tagataccct cgcccggatt aatacatatg
     9541 aaaggattgc aagatattac tttttagacg agtggaacgt atcattggaa cagaggatac
     9601 gaaaaaattg ttatgccctc tgcaaaactc cccgacattt cagggtgttc ccgatcgacc
     9661 gaaccttgga atgatccgcc ctgtccgttc catatactgc tcgtatgctg caccgaacct
     9721 tcctgcaagc attttttcct cgacagggac ccggacgaca tagaggggaa tgaaggtgat
     9781 gagcaggcca aacccggcaa tccagttcca gagcaggagc ggctgggcga atgcccagag
     9841 gaaatgggag gcatacatcg gatgcctgat gtactcgtag accccggtgg tgaccagccg
     9901 ctgtccctgc tgcacctcgg cattgggggt gaaatttgca ccaaggtctg catgggaccg
     9961 ccagaggaga aggagggcgg ctccgaatat gccagccccg acccagccgg tagattccgg
    10021 gagggtgtaa tcggccgggg caaggtacgg ggtgaggatg tagatcccag ggatgatgaa
    10081 catcccgagg gcaggcaggt acacaagcca ggtatcaagc gccggtttcc aatgctgctc
    10141 ttttatcttt ctccggtact cctgggtata ccagagacgg atggctgccg caaggaggaa
    10201 accggagatg taaaggctga ggaagatcgg cggggtcatt gtcgaaggac cactccaggg
    10261 aatcagcata gcgtgctcct ggcatatctt tttttctctg ccgggaacgt tccgagacac
    10321 gggtagtttc agtgatcacc catcactcat cctctaagaa tcatatgaaa atggatcaac
    10381 aatcattact ccaggatcac tcctttacaa tcgttccccg tttcatgagc tctcccctca
    10441 acaggttctc ttatgtcttc gtccggcaca tgatcctgta gaaatgaggg agcctgccgt
    10501 caagaaaacc ctgtactggt gcgaacagtg caatgtgccg ctcatcggcc ggagctgcgg
    10561 ctgtggagcc cagggtaaag cactccccct cctgcaaccc tacgatgttc gtcccgctct
    10621 ttctgccgac cgggagctga tccacaccct cctcattgag cggttcggtc ccatcccgct
    10681 gccgcagatc atcctcctga acaagactgg cggggtcgac agggctgacc tcgtgatcat
    10741 gcacggggac cggttcggct ggctgacctt tgacccggtg acccgccggt tcagcctcga
    10801 tatcgcccct gaagcactcc cgtatctcat tccccatgtt acccagggga tagtccgcct
    10861 tgggaccgat gctgatctcg gcccggaggg cggtaggatc ggagggaaac ggttccccct
    10921 caaaaagccg gcaggggagg ggaccgtgat cgtaacctac aagaaccggt tcgggaccgg
    10981 cgtcgtgaaa gaagggttta tacgggtcaa ggaggtgata ccggtgacgc cgaagacacc
    11041 tcccgacccg gactgggaca cggccatccg gcagaaccag taccatttga agaacctgga
    11101 acgaaacgcg gtccgcacca taaaacggca cctccatgac aggccctgcg tcaatgtctc
    11161 cttctcggga ggcaaagaca gcatggcagc actgctcctg gcaaggaaag caggcgtaga
    11221 gaaggcattc ttcctcgaca ccggcatcga gttccctgag accgtggcgt ttgtcgagtc
    11281 gcttggggtg gaggtgatcc ggcaggccgg tgacttctgg aaggcggccg agaaggccgg
    11341 gccaccggga aaggatcacc gctggtgctg caagctgcag aagctccacc ccctgaagat
    11401 ctacctcgag acgaccggcc cctgcctcac ggtccaggga aaccgctggt acgagtcctg
    11461 gaaccgggct gacctcgacg aaaccagcca gaacccggcc aatcccctgc agctgaacat
    11521 ctcgccgatc cggaactggc gggcgctcga agtattcctc tacctctggt ggcagcatgc
    11581 ccccatcaat cccctctacg agcaagggat cgagcggatc ggctgcatgg tctgcccagc
    11641 gatgctcgag agcgaatacg agatactcag gaccctgcac cccggggaga ccacgcggtg
    11701 ggatgcgttc ctcgaccggt gggccgggaa gaaagggttc cccgaagagt tccggagctg
    11761 gggcctctgg cgctggaagg cactcccgcc caagatgcgg gagctctgca ggagcagggg
    11821 tatccccttc aatgaagatt tcaccctgaa gaccgttccc gggtggagaa cgcccggggg
    11881 agagggaaga gagagtacca tgaaaccaga aggagagaga aaggatcgcg aatccttcgc
    11941 cgttgaagag atccggaagg acttcccgat cctcggagag atcatctacc ttgacaacgc
    12001 cgccaccggg ttctccccgg agccggtggt cgaggcgatg atcgagttcg agcaccgcta
    12061 ccgagccaac gtcggacgcg gggtgcaccg ctatacccgt atcgccaccc agcgatactg
    12121 gcatgcccac gagaaggtag caaatctgat cagtggcaac gacgggacca cagtattttc
    12181 gaaaaacacc accgaggcca tcaacatggt cgcccatggc ctctcctgga ggccggggga
    12241 ccgggtcgtc acgaccatcc tcgagcacca ttcaaacctc ctcccctggc gggaactctc
    12301 gcgcatgggg gtgacggtcg agatcattgg gatcgctgag gatttccagc tcgatctcga
    12361 tgccttcagg gcagccctct gcgaaccggt acggctggtc gccatgaccc atgcatcgaa
    12421 tgtgcttgga gtggtcacac cggtggcaga gatagcccgg atgtgccgga agaacggggc
    12481 actgctggtc gtggacgggg cacagtcggt cccccacctg ccggtcgatg tccgggcgct
    12541 ggactgcgac ttcctctgct tctccggcca caagatgctc gggccgaccg gtaccggggt
    12601 cctctggatg aaggaaccac tccttgagcc ctccatcctc ggcgggggaa tggtcgagtc
    12661 ggtcactgcg gacgggtacg tccctctcgg ggggtatgaa aaatacgagg cggggacccc
    12721 gaatatcgcc ggggggatcg gccttggcgt tgccgcagag tatctcatgg caaatgggat
    12781 ggatcggata caccgccatg agagcgccct taccacccgg ctgattgacg ggctgcaggg
    12841 gatcgatgat gtccgggtat atgccccacg ggatcctgat gcacggatcg gcgtggtctc
    12901 gttcacgatc aaagggatgc acccccacga agtcgcccag cagctggacg accaggctga
    12961 gatccttgtg cgatcagggt accattgctg ccagccgctg atggaatccc tcggtctccc
    13021 ggatggcacg gtcagggcga gcatagccct ctataccacg gaacaggaga tcgatctcct
    13081 ccttgccgcg gtacgggcga tctcaagggg gaggtaatcc tgccaggaga ctggaaggcg
    13141 aatcctccct gatgcaggaa gagaaggacc ctccttcttt actgtccgca ccctttttaa
    13201 tatgaaaatg cctccggcat tttcatctcg tatacatgtg tccctcgaag gaatcaggta
    13261 ttttttaact tggtgactga ggtaccctgg agagagttca gggcagcatt tccattcccg
    13321 gattccctgc cgaaattcac cactatagag aagagagcct taacgaagag gcatcactcc
    13381 tgcctgcccc cgccatcctt gtcgcccgca tggccagtac aaaggaggat gccagcccat
    13441 gtgttttttt acccgccaga attcatcata gagtaggtag tatggcggaa gatagtgaac
    13501 tgagggagga gatcctcaca ctcaaggagg aacgggatgc ggtcatcctg gcccataatt
    13561 accagatcga cgccgtgcag gacattgccg attttgtcgg ggactcgctt gagctggcac
    13621 gggctgccgc gtcgcaaaaa gcccctgtca tcgtgttctg cggcgtggac ttcatggcag
    13681 agactgcggc aatcctctct ccggaaaaga cggtgctcct ccccgccgcc ggtgcctgtt
    13741 gcccgatggc agagatggtc acagccggtg aacttgccgt cctgcaggag caatatcccg
    13801 atgctgccgt ggtcgcctat gtcaacacca ctgcggaggt caaggcagtg agcgatatct
    13861 gctgcacctc ggcaaatgcc gtgaaggtgg tggaatccct gtcagagcag caggtgatca
    13921 tggtgccgga ccggaacctt gcacggtatg tcgcccgttt caccacaaag gagatcctcc
    13981 catgggatgg attctgtctc gtccatgaac ggttcacctg caaggacatc cagagagcga
    14041 ggagccgcca ccccggttca gaagtgctcg tccaccccga atgccggccc gaggtgatcg
    14101 atgcagccga ccgggtcttc tccacgtccg ggatgatccg ccatgtctgt gcagatccgg
    14161 caggagagtt catcatcggc accgagatag ggatcctgca ccagctctca cgccggtgcc
    14221 cggaaaaaca ctgctacccg ctctcctcgc gtgcgatctg tgtcaacatg aagaagacgg
    14281 acctggaaaa ggtccatgcc gccctcgata ggatgttacc ccgggtccag gtccctgaga
    14341 ggatcgccat cccggcacgg agggctatcg agcggatgct tgcacttccg taaagccgga
    14401 ccggttatcg gaccttgcta accttttctt tcgaatcctt tatgatcgcc ttcatgtgcc
    14461 gtgcggccgc cgtgatatca tcctgcgcca tgaccgcgga gatcaccgca accccttcag
    14521 caccggcagc gatcacctcc ggcgcattcc ggggagtgat cccgccaatc gcgatcacgg
    14581 ggagtgtcac cgatgcacaa atcgcctgta actctgcaat cccatggccg ggcccggcat
    14641 catcctttga cgtggtcggg aagatcgggc tgagcgagag gtagtctgcc ccggctgact
    14701 cggcagcgac tgcctcattg acgttgccga ccgagacccc gatgatgaac ccttccgggg
    14761 agatcgaccg ggcatccctg atagggagat catcctgtcc gatatggact ccgtcagctt
    14821 ccgaggcaag ggcgatatcg atcctgtcgt ttacaaagaa gagcaccccg gcttcccatg
    14881 ttatctcccg gatctcgcag gcgacagcca gcaggtctct gccagggagg attttgtcgc
    14941 ggagctggac tgcatcggcg cctccctcca ccaccctgcg ggcaatcccg gcatggggca
    15001 tcccgcgggc tattgccgga tcggtgatca cgtaaaggtc aaaggccatc cggcacgctc
    15061 accttcgccc cggctgcaag gtcagccgct cccagcctgt acatcgcatc gaacagtgcc
    15121 atccggaacg agtacggacc aaagcacccc tccatagccc tctctccggc aagcccgaac
    15181 gcagcaaggg ctgcaacagc ggcagtggtt gtgtcgtcgg agatcgcggc aaacgcacca
    15241 gtgaccgagg ccgccatgca gccggttccc gagaggtttc ccatcatcgg ggtgccgttt
    15301 gcgacaagga aggtgcggcg tccatcggag acgacatcga tctcacccgt catcgcgaca
    15361 accgtcccgg ttgcccttgc acattccctg gcgacctcga tcgggtcccc ggacactccg
    15421 cccgaatcaa cccccctgac cgtccccccg agacccgata gcaccccgat ctccccggta
    15481 tttcccttca ggatggccac gtcaagctca tccaggagca ggaggacggt ctcagtccgc
    15541 atcctggtag ccccggcccc gaccgggtcc aggatcaccg ggatcccgag ttcgtttgcc
    15601 cgttcgccgg caaggagcat cgccccgacc tgggcttgtg tcagggtccc gatattcagg
    15661 accagggctc cggcgagacc ggccatctcg gctgcctcct caggtgcctc tgccatgacc
    15721 ggggcagcac ctgcgcagag ggtgatgttg gcacagtcgt tgatggtgac gcggttggtg
    15781 atatggtgca ccagcggccg ccccttacga acgagggcaa acaggttgcc atacaccgta
    15841 ctgtccatca agagccctcc cctccgtgaa ctgccacgtt cccccgtact tcatccatgc
    15901 tcccgctccc ttcatccatg ctcccgctcc ggtaccccat caggtcgagg gtgacgtagg
    15961 agaagccggc ctccttcaca agggcgacga tcgtctcccg tgctgccacc agccggggga
    16021 tatctgcttc cggaacttct attctcgcga tgccgccatg gttccggacc ctgctcccgg
    16081 cgaatcccgc ttccgcgaga ccggcttcgg cctcctccac catgtggagg ttctcccggg
    16141 tgagtgcctc cccgtatggg atccgggagg ctaggcaggc ggacgatggc ttgtcccaga
    16201 acgggagccc ccgctcccgg gcgagctgcc gaatctcagg cttcgtgatc cgggcttcaa
    16261 gaaacgggtg gatgatcccc tcctcggttg ctgcgcgggt accggggcgg tattcagatc
    16321 gatcggactc agtgacaccg tcggcaatgc aggagatgcc ctgctcgtcc gcccatgccc
    16381 ggaggagttc cgagaatgac ttcttgcagt gataacaacg gtcaggaagg ttccgtgaca
    16441 cgatctcccc gggaaacggg accgtgataa tcccgagtgg tatcccgagg agcccggcca
    16501 tctcccgggc ctccgatacc gccgatcgcg ggaccagggg gctgtcgatg aagactgcct
    16561 gcagattatc accgagtatc tcttttccca gagcagcaag gagaccgctg tcaacccctc
    16621 cggagaaaga gaccatcagc tgatcgcatc cggagatacg cctcctcaat tgtcccatcc
    16681 gttcttcgat tgtggtaccg gtcatcctgc gcacctgtct ttgcaggggt attgttcccg
    16741 ttgcagcagc aatcatgctc cttttggtac caaatggttc gggatacaag attatccact
    16801 ttcccatcaa tgactataat ggagacagaa tcgaggatgg gttcattttt tatgccatta
    16861 cgaagaactc cggagcatac ccaacagatg accatgagga agcaacggat cgacagaaac
    16921 gtattccgga ccgctggcgg caagggatca gagtggatgc gaggaggaga tagccctgct
    16981 ctccgtccgt gagcatgaac gctatctccg gcagatgatg ctctttggca aggaggggca
    17041 ggagcatctg caggatgcaa cgatattcat tgccggggct ggagggcttg gatcgccggt
    17101 ttcgatgtac cttgccgtcc tcggggtcgg caccctcatc atcgcggaca atgaccgggt
    17161 agaccagacg aacctgaacc gccagatcct ctaccgcgat caggacattg ggcggcgaaa
    17221 agcagcggtc gcaggagaac ggttgcagga gctcaatccc gacatcacgg tgagagcagt
    17281 tgatgcaacc atcgatcaga acacggtctc ctccctgaca agggatgctg acggcatcgt
    17341 cgatgccctt gatgatttcc cgacccggta tctcctgaac agcgcagccc tggagagagg
    17401 gatccccctc tttcacggag ggatcagcgg cttcttcggc caggcgacca cgatcctccc
    17461 gggaaaaacc ccctgcctcc gctgtatcat cccccgcccc ccgccagccg aggtctttcc
    17521 cgttgccggt gtaaccgcgg gatttatcgg catgatacag gtgaccgagg tctgcaagta
    17581 cctcctctgc cggggtgatc tcctcgccgg ccgcctcctg ctctgggacg ggatgcaggg
    17641 ccggattgag gagatcccgg ttgaaacgga tccagattgc gaagcttgtg caggaaaata
    17701 tacagcgatt tcgtgatacc catgaagatt accgtccgat ttttcgcccg gttcggagaa
    17761 ctcctcggga atacccggga gatcgaggcc agggaaggca tctcggttat tgacctcgta
    17821 aaagaggcag catccgggaa taaggcaggg ttcgatacga tctttgacga agaaggtgca
    17881 ttcagggatt ttgtgatcgt gatgcggaac aagaagagga tccggacaca ggatgccggc
    17941 tccatcccgg tcggtgaagg ggatgagata gcggtcttcc ctcccgttgc cggcggataa
    18001 tccggctttt tgggatacaa tcctgttttt ttccctgccg cccaggtccc tcatctcctc
    18061 ctggaaactc ccccaaagcc tcgttcagaa ggtcgccgga ctcctgcttc gccatcttat
    18121 cgatggaact ggcacagaac cgcatcgcgt tctgccaggg aaagttggat tatatggacg
    18181 aagctctgtc gagagaattc cggaaccgga tcagctcctc gcagaactcg cccaggtatt
    18241 catccatcga gagactgcct tcctggaacg aacagtcctc ctcctcaatg atcttattga
    18301 tcctctcccg gctcggcagg agcagatcga tcggcacccc ggcctgctct gaccccctga
    18361 tgagtgcttc acggaacagc ccgaggcagt acccgatccg gtaatggtat gcatcccgtg
    18421 tcttttccag gtatccggcc accctctcag tctcgatacg caggaaatcc tgccggatgg
    18481 tctcgtccgt gcatttcccg agcatgtacc ggtaatgggc atacgggtac atctcctcga
    18541 gctcagccgg gacgatcccg caggtcccga acacaatgat atggtaccgc gagggatcga
    18601 gcacctggga gatgatcatc cggatcagct ggtggctcgg gctcgtactg tagggctttc
    18661 tgaccgcaca ggggatgaag attgcaatct cccggggctc tacttcatat tcgtcgatga
    18721 tatagcggta cgaccgctcg aattcgggga ggtagaaggg tggacctgtc aggacggttc
    18781 tctcgccctg tccgggatct cccttctcct gcccgcccat gatgctcctt caaaaatttg
    18841 tcaggggacc tgaaagaatc accggatgag cgctgggggg tgaaccggga accaggctga
    18901 tgcaggacgg gatgattcgg atggccggtg cctcacggat aatccgaaca attgatggtt
    18961 cccgtttgca aagatggacc tatgagcgag atggaccaga tagggaagat cagtctgggg
    19021 aagaatgcat tcccgttccc catgccggtg acccttctcg gggcgaccgt gcacgggaag
    19081 ccgaatttca tggcacttgg gtgggtgagc cgcgtgaatg ccaatccccc gatgatcggc
    19141 tgcggtgtcg ggaggcacca ctatacctcc gccgggatca aacagaacaa gaccttctct
    19201 gttaatgtcc ccggccgttc gatgatggcg gaggcagatt actgtgggat agtctcgggg
    19261 gagacgacag acaaggccgg ccttttctcc gtcttttacg gggaactgaa gactgccccc
    19321 atgatcagcg aatgccccgt ctgcatcgaa tgccagctgg tccagtcagt gacctacccg
    19381 acgaacgaat tcttcatcgg ggagatcgtg gggtcctaca ccgaagagcg atacctaacc
    19441 gggggaaaac cggatatgaa gaagatcgat cccctcctgc tctcgatgcc ggataaccgg
    19501 tactggagcc tgggagagta tgccggggat gcgtggagca tcgggaagtc actgaaaaaa
    19561 taaccgtatg acgagaaaga cagggacgaa cagggggggt cagcccgctg atccagatcg
    19621 acaggaatac ggtaatcatg actggcgaga gaggcccctt cagccagatc ggcaaaggat
    19681 gaggttcctg acaggagcgt cagcccgctg atcggaaatc cccatagcct gtctcgtacc
    19741 ctcccgcgat gaccgcggcc ccgaggagag gcgcatctcc tccaagctct gacaagacga
    19801 tctcagggag gggaaggaac cggtcgatga actcccgcat gtccgggagg aggagatcgg
    19861 cattggactg catgacagcc ccatcaagga tgatgatctc cgggtcgtac gcgacaatga
    19921 catccgagat gcccctggca ttgatccggg agagctcccg gacaaaacgg agcacgtcat
    19981 cgtccccctg ccgggctgat gcaaagatct cctctgccgc atcaggcccc cagtgcggct
    20041 ttgcatggaa ccggcaccat tccgcaaaga accaggggag gaacctcccg gaggcatacc
    20101 cctcccagtg cccccggtgg ccgcagctgc aggggagatc ataggtattg tcgacaagga
    20161 agtggccgat ctccgcggca ttcccctttc tgccgaggag caaccgtccc cttgatacga
    20221 cccctccccc aatcccggtc gagatcgtca cgtagacaac atcctgtttt ccctgtccct
    20281 tcccgtaatg catctccccc agcactccgg catggcaatc gttgaccatc ctgacagggc
    20341 aatgaaatat cccagagagc tcctcacgaa gaggcacatc ccggaaggcc atgttcggcg
    20401 gattcacgag cacgccccgg gtgatatcga ccggcccggc aacagagagc ccgatcccgg
    20461 agagagattc ttccccgtgg gcggccctga ccccactgat cagggatgag aggaacccgg
    20521 caagccttga gggatcgctc tcttttgccg gggtccgtgc ctccctccgc tccccaatct
    20581 gcccttcctc cccgataatc gccacccggg tgcgtgtcgc ccccaggtcc actgccactg
    20641 cctggaccat gatgttccga tgtttgcctg cccggagcat aaggttcgcg gcccatccac
    20701 aaagggtatg aaggggcgga gggcagagct tataaacaga tggacctgaa gggttgggtg
    20761 gaactggacg ggaaactgat ctcattccgg gaactggaga ccctgctccg caccgatccg
    20821 cggacagttg cccggttcgg tggcgaattc ctcctctcgt gggatggctg catagcccgc
    20881 gaccacctcg ggatcattcc cggaccatgc cctgccggga ccatctcctg cgaaggaaga
    20941 gagattggga aggtatgtcc tgaccccccg gcaatgtccc ttgaagaggc gatcgtcacg
    21001 gcagtgcggc ttcggagcac cgaggggatc tgtgcccttt cgggtggcgt ggactcatcc
    21061 ctcgtggcat tccttgcaca acgcccctgc atcgcgatcg gacttgctgg atcgcatgac
    21121 cttgcacggg cacggctggc agcagaagcg atgcagctga cctgtacctt catcgaggtg
    21181 acttccgatg atatcaggga agggctccat gcaacgatca gggtgatccc tgaaccgacc
    21241 ccggtcaata ccgcaattgc catcacccag tacctgatca cccggtctgc gggagagctc
    21301 gggcacgaac gggtgctgac cggccagggt gctgatgagc tcttcggagg ttacgcccgc
    21361 taccggaccg ggattgatcc cgctggccgg ctcgcccgcg actttgccgg gcttgccgcc
    21421 cagggactcc gcgaccagtc agtggccgcg ctgaacggga cctacctctc gcagccgttc
    21481 cttgatatca gggtggtgcg cgctgcacgg tccctcccgg ccagcgggat ggtccgggac
    21541 ggggtcggga aatggccgct ccgcgaggtc gcagcccgcc acatcccgcg ggaaatcgca
    21601 tggcacggaa agaaagccat gcagtacggg accggtattg ccaaggtact gcaggggttt
    21661 gcacgggaca agggttataa aaggtcttta caacggtact tagatcagat cagaacgagc
    21721 ggggaggcac ggaatggtat ctgagaagga tgtagcacat atcgcagagc ttgccgatat
    21781 cggtatcgag aaagaggaac tggcagaatt cacgggccgg ttcaacgaga tccttgacta
    21841 ttttgatatc ctcgaccagg tggagcaagg atcacgcgga gggaccgacc ttgtgaacat
    21901 cttccgggat gatatcgtgg aagattccct ccctgtcgag gaggtccttg ccaatacccg
    21961 cgagaaggaa gatggatttt tcagggcgcc gagggtgatg tgaatggaga ccctgacgtt
    22021 cacgcgcgac gatcggtaca acgcctttat caccatgctt gacaaggctg ataccggtca
    22081 ggggaaactc tccgggatcg gcatcgcgat caaggacaat atctccaccc gcggcatcca
    22141 gaccacctgc gcctcccgca ccctggaagg atacctccct ccctacgatg cccacgtggt
    22201 cacgctcgta aaaaaggccg gtgcaggaat tgtcggcaag acgaacatgg acgagttcgg
    22261 gatgggcacc acgaccgaga acagtgcatt cgggcccaca aaaaatccgg ccgatacctc
    22321 ccgggtgccg ggagggtcct cggggggaag tgccgctgcc atcgcatcag ggatggtcca
    22381 gatggcgctt ggcacagata ccggtggatc gatccgctgc ccggccgcat tctgcggtat
    22441 cgtcgggcac aagcccacct atggcagggt ctcgcggtac ggcctggtcg cctatgccaa
    22501 ctcgctcgag cagatcggcc ctatggccag gaccgtctcc gatatctccc tcctcatgga
    22561 ggtcctggct gcgtatgatc cccgcgactc caccagtgtc aactgtccct atgcccatat
    22621 ccctgatccc gatgtcaagg gcctcaagat cggggtcccg gaagaatact ttggcgaggg
    22681 ggtcgatccg agggttgcct cggtggtatg ggacgggata tcactcctgg aacggaaggg
    22741 tgccgatatc atcacctgcc ggatgccgag catggcctac gccctggctg cttattacgt
    22801 gacctgcacc agcgaagcca gctccaacct cgcccgtttt gacggtgtca ggtacgggcc
    22861 cccggccgat accagacaga cctggcatga atcgttccag gagcggagga gggacgggtt
    22921 tggaaccgag gtgaagagga ggatcatgct cgggaccttt gcgctctcct ccggatacta
    22981 cgggaaatac tatgcaaagg cccaggcagc ccgccagcag gtccggaacg acttcctccg
    23041 cctcttcagg gaagtcgacg tgatcgcagg acccaccatg cccaccgtcg cgttccggat
    23101 cggcgagaag accgatcccc tctcgatgta cctctccgac atcctgaccg tcccggccaa
    23161 cctcgccggg ataccggcca tctcggtccc gtgcggcacc tccgagggtc tcccggtcgg
    23221 cctccagatc atgggacccc acttccggga cgaacgggtg atcgatgtcg cctatgcctt
    23281 tgagcagggg gtgtcccgat gactggaacg gcagacactg caggacagga aagcagggtg
    23341 atggacttct ccgatgacga ggtgatcatc ggcctcgaga tccactgcca gctcgacacc
    23401 ctgaccaagc tcttctgcgg ctgctccacc gattaccgcg atgatggccc gaacacccac
    23461 gtctgcccga tctgtctcgg cctcccgggc tcgctcccgc gggtgaaccg gaaggcgatc
    23521 gagtatgccc tcagggtggc aaaagcgttg aactgcacca tcgttgagga gtcggagttt
    23581 gcacggaaga actacttcta cccggacctg aacaaaggat tccagatcac ccagtacgac
    23641 cgtcccctcg ccgtgaaggg ctatcttgac atcgaaggtg atgacggtga gaaacgggtc
    23701 cggatcacgc gggtgcatgt tgaggaagat cctggcaggc tcgtgcacaa gggcggtgcc
    23761 gaccgggcga agtacaccct cgtcgattat aaccgggccg ggattcccct gatcgagatc
    23821 gtgaccgagc cagacatccg gtcgcccaag gaagcccgga aattcctgaa caagctcagg
    23881 gcaaccctcg agtatctcgg tgtcttcgac agcgagaaag aggggtccct ccgtgtcgat
    23941 gcaaatatct ccatccagcc acgaggctgg tttgaccggg aaggcaaggg aaaggcagac
    24001 cgggggaggg cagaggtcaa gaacatctcc tcctataaag gggtggagaa agcgcttacc
    24061 tttgaggtga cccgccagaa gaatgcgctc cggcgggggc agcggctcgg gaccgagacc
    24121 cgccattttg tcgagacacg gggagtgacc acctcttcgc ggagcaagga gatggagcag
    24181 gagtaccggt acttcccgga gcctgacctc cgccccctgc gggtctgtgc ctgggctgac
    24241 gagatcgtgc tcccggagct cccggatgcc aggagggcac ggttcctcac ccagtatgga
    24301 tgctcgccca tccatgcacg gacactcact ggtgatctcc gggtggcgga attctacgag
    24361 caggttgccg gaatcgaccc ggtgcttacc gccacgtggg ttgccgatac ccttcttggt
    24421 gagctcaact accgggacat gagtgtcggc agagtatccc cggactggtt cggtgagtta
    24481 atagtactgg taaaggaagg gagtgtgact gacaagggcg gggttgaggt actccgatcc
    24541 cttctcgacc agatcaggga gtcggggtcc tgtgaaactc cgggtgtctg tgtcgggagg
    24601 ctcggactcc gggtcctgga acggaccgca gagaccggct ctgatgacga cccggtcgtc
    24661 aaagcggcac gggaagtggt tgccgagcag ccccaggcag tcgccgattt tgcggcagga
    24721 aagaaggaag cgttcaattt cctcgtcggg caggtcatga aaaagaccag ggggcgtgcc
    24781 aagcccgctg atgtgaacca ggttctgtct gcaatcctcg gaaaagaggg ctgaacgcga
    24841 tgcacctgat cgttgccgag aagaatatct cggctcaccg catcgcctcc atccttgccg
    24901 gcaaaacaaa agtggccacc gacaaggacg ggggggtccc ggtctaccgg ttcggggata
    24961 ccgcggtcat cgggctccgg gggcatgtgg tcgagatcga ttttgaagag gggtactcga
    25021 actggcggag caccgaacgg accccccggt ccctgatcga tgcccggacg atcaaggtcc
    25081 ctacggaaaa gaagatcgtc catatccttc agaaactggc aaagaagtcc agcctcatca
    25141 caatcgcgac cgacttcgat accgaggggg agctgatcgg aaaggaggcg tacgagctga
    25201 tccgccaggt gagccgggag gttcctgtcc ggcgggcccg gttcagcgcg atcacccccg
    25261 atgagatccg gcaggcgttc agcgagacca cggagatcga ttttgacctt gcctcggcag
    25321 gagaggcaag acaggtgatc gatctgatgt ggggggcctc cctcacccgg ttcatcagcc
    25381 ttgctgcccg gaggggtggc agcaacatcc tctcggtcgg ccgggtccag actcccaccc
    25441 ttgccatgat cgttgaccgg gagaaggaga ttgaggagtt cgtctcccag cagtactggc
    25501 agctctcgct catgaccgag aaggatggcg aggagatcga ggcccgccat gccacccccc
    25561 gtttctgcga caaggctgaa gcggtgaggg cccgcgaccg taccctccct ccgcttatcg
    25621 tgaccgaggt caaggaaggg gccaagatcg agcgggcgcc tgtcccactt gacaccacgg
    25681 gattcatcgt tgctgccgcc cgtctcggct tctcggctgc cggtgcaatg cggattgcag
    25741 aggacctgta tatgaacggg tatatctcct atccccggac ggacaacacc gtatatcccc
    25801 cgacccttga cctcggggga gtgctgaaag cactctccgc gaccgagttt gaaggggagg
    25861 tggcctgggt caccacccac cggagacctg cgccgacccg cgggaagaag acgagcaccg
    25921 accaccctcc catccacccc gcctcggccg caacccgcca ttccctcggg gaagagcgtt
    25981 ggaagatcta cgagctcgtg gtccggcggt tcctcgcaac gctctcccct gatgcccgct
    26041 ggaagacgat gaaggtgaac ttcagggcag gagaggagcc ctacaccgcc accggagggc
    26101 agctgaccga gcccggctgg cggaaggtat acccctacag taccgcgaca gagtatatcc
    26161 tcccccctat gaaggtcggc gagcagctcc ccatccggga ggtaaccctc gaagagaagg
    26221 agacgcagcc gcctgcacgg ttcacccaga gcaagctgat ccagaagatg gaggaactcg
    26281 ggctcgggac caagagcacc cggcacgaag tgatccagaa gctcctctcc cgcaagtata
    26341 tcgaaggaaa tcccctccgc cccacgctgg tcggcagggc agtcaccgag tccctggagc
    26401 ggcacgccaa caccatcacc cggcctgcca tgacccagac actcgaagag cacatgcagc
    26461 agatcaagga acagcaccgg agcagggacg atgtcgttgc cgagtcccgc gggatgctgc
    26521 actcggtctt cgatcaactc gaacccaacg aacaggtggt cgggcaggag atcatggagc
    26581 agactgcaga ggaactgacc ctcgggccat gcccggtctg ctcccatgac atcaggatcc
    26641 gccacatgag gaaccagacc cagttcatcg ggtgtaccca ttatcctgac tgcactttca
    26701 atatcgggct tccccaggcc atgtggggat ttgcggtccg gaccgatgat acctgcccga
    26761 tccaccagct ccattttgtc cggcttgtca ggaaggggag caggccgtgg gagatcgggt
    26821 gccccctctg ccaccagatc gcctcgaata aagaagccct cttcctcatg gcatccatga
    26881 ctcctgagat ccatgaacgg atgatcgctg cacacctcta cacggtgacc gaccttgcag
    26941 gcagggatcc tgcgcaactg gccccgctcc ttgggatcag cggggagaag gccggcgacc
    27001 tggtacgtga agcggaaggg gtccttggac tgctcaggcg gagatcggac tgccggaaac
    27061 tcatccggag gatgcttgtc ccgagaaagg gcaggagccc ggccagtgtg atcaagaaac
    27121 tccaggaagc agggatcaac gacatcgcct cccttggaac ggctgatcca gccctgctga
    27181 agagggtcgg gattgctgac aaagaggcag agtcgatcct ccatgaggca aagatggtcg
    27241 agaatgagcg gttcctgaaa caggccggca ttcccgcggc gagcatgaaa aaatattttt
    27301 ctgccggcat cagctccccg gaggattttc tctcctccca cccggtcttc atctctgaca
    27361 ccaccgggat cagcctggat accgtccaga aacatgtaag ccttgtcgcc gcggcaaaag
    27421 ggaggccggc accggtgcga tacaccaagg ttcaggtggc gaaagggaga gaggaactca
    27481 cctcgctccc cggtttttcg gaagaggact tgcgccggct ctggagagca gggatcatca
    27541 acggcctcct ccttcttgaa tcagatccgg agagtcttgg tgagcggacc ggtatctccc
    27601 ctgcaaggct ccgcaggtac caggccgtgc tgcaggaaag ggcaaaaaag gcggagcccg
    27661 atattatagt ggtataggtg ctgatgatgc atacccgatg gaagaactgg gtccatgtga
    27721 ccaagcttga ccctgacaag atgctccctg cgggggcgat cgcagagatc gtctccagcg
    27781 ggaccgatgc cctgatgctc tctgggacgc tcaatgtgac ggccgagaac atggcggcgc
    27841 ttgccaagga ggcacgggca tcagggcttc cgctggtggt ggaacccgcc ggcccggagg
    27901 cggtgctctg ggacggcgtg gaccttgtct ttgtcccctc ggtactgaac tctccggaag
    27961 tccagtggat cgtggggaaa caccgttcct ggatccagag gcagcaggtg gagtgggaga
    28021 aggtggtccc cgaggcatac atcgtgctga atcctgattc tgcagtgggg aagctgacga
    28081 aggcgacctg taccctgacc ggaaaggagg tcgcttcatt cgcctccctc gctgatcact
    28141 acttccactt ccccatcgtg tacatcgagt attccgggat ctatggcgat cccggggtcg
    28201 tcagggagac agcggctgcg gtggagaacg cggtcatcta ttatgggggc gggatcaatt
    28261 cggccgcaaa agccgccgag atggccaggt atgcagacac catcgtggtc ggcaatgccg
    28321 tctatgacca gggcatcggg gtcctgaaag agaccgtcag ggcaatccag tgatcttccc
    28381 cttccatgcc tgaaatcgag atagccctcg tctctccgct ctatgaaggg aacgtcggat
    28441 ttgccgcacg ggtgatgaaa aatttcgggt tcacccggct tgtcctgatc gatccttgtg
    28501 agatcgggga cgaagcgttc atgcgggcag cccatgcctc ggacgtactc gagaacgccg
    28561 aacggctctc ctttgaagaa gtctgtgccc ggagcagcat cgtgatcgct accacgggcg
    28621 aggtgagcaa gtcggtctgc aacccgatgc ggatgcccta cttctcccca aacgagatac
    28681 gggagcttat cgctgatatc gatggtcgca tctccatcct ctttggaagg gagaactggg
    28741 ggctgaacaa cgaggagatc cagaagagcg atatcgtctg caccatcccc tcatcaaagg
    28801 aatatccgat cctgaacctc tctcatgccg tggggatcgt ctgctacgaa cttgcccata
    28861 tcagaagggg ggagtatctc cttgccacga gagaggagat ggaatacctg tatgcccata
    28921 tcgactcata cctcacccag atcgatcacc ctgatttcaa gcgggtgcca accatgctcc
    28981 tgatccggag agtcctcggc cgtgccatgc tcacctcgcg ggaagcaacg acgctgcatg
    29041 gccttatgcg gcgatccgaa tggaagatca acccggctgc attcgagcgg attaaaggga
    29101 actcttctgg tgacgatgac cagtcttcgg ggtaatcgtg acatccacgc tgcaatcgat
    29161 accagatcct gagccaatac ttccacatgg cgcaagacgc cttgaatttt caggatctga
    29221 aagccctttg gaaggtgaag tcggactatg agaagttgga tgacttaccc aatctttcag
    29281 gatacctgat atacgggagg acctaatgaa acagagcgtt aaaatcgaac gagatcaata
    29341 cggtctggcg aggacagatt ggggcgtcat ttatcgaaga tggggcagtg caggtgattt
    29401 agggcgatgt tcctggcagg caccacatct gccgtttccc cctttttgag aatactggcc
    29461 taggaaagac aggtacctta taatttcaga gagggaatat cagacaataa aagtacctcg
    29521 cagaggagag gcgctatgca ggaattcgct ggagaaggca acagggacta tacctgcagc
    29581 tatggaacga aataccttct gaaacagatc tcagctttga gaaaacagat ccccggtgtc
    29641 atagctgcac acgatattga acatatccac cagatgcggg tggcgacacg acgcctcagg
    29701 agtgccctgc ctctcttccc tctctgcctt ccacaaaaaa agattcaacg gtggcggcgg
    29761 gatctgcgga atactgcccg gtccctgggg aaggcacgtg atttggatgt ccagatctcc
    29821 tttctgacag aatttattgc cgacctgaaa gcggggaggc cgatggaaaa ccgggttctg
    29881 ctcccttctg tggttcccga aacaccccct cccgtgccgt tcaacccaaa atggtatacc
    29941 ctgtccatct catatcggaa actagtgacc acattccagt ctgctctctc acgggcctgg
    30001 aactatttca gtcatacggc agtggaaggg gatctggcag gtggggactg cttctcctgt
    30061 caggcagacc tgataccggg cctcgagtac ctcctcttca ggaaaaacaa ggagaggtca
    30121 gactaccaga gtgaggtcaa gaagaccatc tggcaaatgg agaaggccgg gatcctcgga
    30181 gccatggaga agtatcttca gaggcacagg ccggttgaaa gggcatcact gcaggtgggg
    30241 cgcgagacct atgccactgc cttcacttcg atagcattga ggatagagga ggtactttct
    30301 ttcagtgagt cattggctga tcccgggaag atcagggagc accatgccct gcgaattgcg
    30361 gtgaagcggt tgcggtatac ccttgaagtc tggcgggatc tctttgatgg aacgattaac
    30421 aaggagatcg gtgccctgaa ggaattccag gatctcctcg gagatctcca cgactgcgat
    30481 gtctggactg aataccttcc ggagtttctc aagggagagg aggaacgatg cagggttttc
    30541 tttggaaacg acacccattt taaaagcctc gtcccgggta tcgaggctct tgcccgggaa
    30601 cggagggaac gcaggggaca actccatgag atcaccctca ctacctgggg tgaactcgaa
    30661 agtcgacagt tctggtatgg cctgatggaa aagttcatcc tgcccctcgc actgcgtgaa
    30721 gatcttccgg tcaggattgg aatccttggt aatatttcgg gagatacaac agccctccgc
    30781 cgcgtcctcg ctgaatgtta ttcgcgggat gcatcgcttg tcctgaacac gggcacctcc
    30841 ctgggatcac cccgggcatc tgagaagacg gtggatctac tgagagaaga aggcgttgtc
    30901 agtattgcga cagccaacga tctccagatc ctcgacggga gttcatcggt catgacagat
    30961 ggtgattccg gtgttattca atcccggaag agacaatcga agaaaaccag gaaattcttc
    31021 cgcaccctcc cgacttcact ccgggtcttt gttgccggaa aaagcatcct gatcacgggt
    31081 gactcatcgg aaaccgccat ctggagcttt gatgagaaaa caaccaccga gagactgtgc
    31141 gagattgcaa acgaggcccg tgcttccgtc atcgtctccg gcagctcccg catgcccttc
    31201 acgattcggg catgtgatgt ccttttcgtg catcccggtt ctgtggggag ggagggagtg
    31261 cccttctcca cattttccat acttgaggtc ggactgggcg ggagtatggc cgtaacccgt
    31321 catgagattg tgattgatcc ggtgggaccc ggatccgata gcacgctacg gcaacctgaa
    31381 tcctcatcac cctcgctggg ggaggaggga cagaaccacc agccggaaag gtgttgacgg
    31441 gggaaaaatg caagggggcc atttcattcc agactcctga gaaaaccggc cataagaagt
    31501 gttctcaaat aaaaacaagc acccgcccgg gtgatcatac ccgggctggt actgcaccct
    31561 gaggcggtat cacatccttc atatgacggc tccgcaggaa gactttgacg atctcatcaa
    31621 caaggagcag ggcaatagca gcagcgatac agagcagcca ctggctgcct tcgagagagg
    31681 tcagtcccag cccgcgctgc aggaagtcca actgtgtcgg caggaagatg agcagcagag
    31741 ccaggccgaa gagcatcgac tgatttcgat catgcagaac atcccggttg agtgcgctct
    31801 gggtttcact acggcaggca agaccgcggg tgacacagaa gagagagaag gagacaaaag
    31861 ccatggtggc agcgagcgtg aaccctgtcg actcggcgac tgccgcgagg gacagggtgc
    31921 cggctgcggt gagaaccccg atgaatccga tgcgtaacca ctgggggcgg ttcaggatgg
    31981 gttgtttcaa cggccgcggc tttttctcca tcagccccgg ttcgggtttg tcaaagccca
    32041 gcgcaatggc aacgggtacc tgcacgaaga agttgaccca gaggacaacg aacggcgcaa
    32101 acggcacacc gcccgtaatc acgaagattg ctgcaccaag gtatgctgcg atgtatccca
    32161 cgagcgatgt catctggaag cggacgaaat tgaagaggtt cccataaata gccctgccga
    32221 actcaaccgc cttgacaatc gtggcgaagt tgtcatcggt caggatcatc actgcagccc
    32281 ccttcgagac ctcggtgcct gtgatcccca tcgccacgcc gatatcggct tttttgagcg
    32341 caggcgcatc gttcacccca tcaccagtca tggcaacaat gttctgctgc cgttgcagga
    32401 gatcgacgag gcggatcttg tcctccgggg caacccgggc caccaccccg atatcggaca
    32461 gctccttcag gagctgctca tcgctcatgg ccgcgaactc agcgccggtg agggccttac
    32521 ccacgatgcc caattcgcgc ccgatagcag ccgcggtcac ggcatgatcg ccagtgatca
    32581 tgcgcacctg gataccggcg ctgtggcact tcgcgatggc atccctggcc tccgggcgcg
    32641 gcgggtccac gatgcccacc atggccagca gggtgagatc tctcacttgt tcgataaggt
    32701 tggcattcgg gtcaaaggtc ttgggatcga agtcacggcg ggcgaccacc atcacccgtt
    32761 cccccccctc tgccatccgg tcattctcct tgagggccaa aggacggttt tcctcaatag
    32821 caggcaacac accgcctcct ggcatccagt acgagctgct gcgggcaatc agcacatcag
    32881 gggcaccttt gacgaaacaa cgcacgaccg gtttgccatc ctcattggtc atggcatgga
    32941 acgtggccat gaacttgtaa tccgaatcga acggaacctc ggcgatacgg gggtacatat
    33001 cccgtgcacc gtccacgctg atgccccctt tctcagccag cacgataagg gcgccctcgg
    33061 ttgggtcacc gatgagggta ccgttatcaa gccgggcatc ggcgcacaac gccatgggca
    33121 ggaggattgg atcgaggtcg atcctggcac ccccggcgct cagcagcttg ccctcagtgg
    33181 tatacgacag cgttcccttc tgccagggtt gtttttctgt catacccgtg gacagctcca
    33241 tggtcccctg cgtaccatac ccttcaccgg tcaccctgaa gcggttctgg ccggggatgg
    33301 agaactcacg ggccgtcatc ttgttgagcg tcagcgtgcc ggtcttgtcc gagcagatgg
    33361 ccgagaccga acccagggtc tcgaccgacg gcagccgctt gacaatcgca cccagactgg
    33421 ccagagcccg cgtccccatg gagtacatgg tggtgacgac tgccggcagg ccggtgggga
    33481 tagccgcaat cgcgagcgcg atgccggcga cgaagatcgt atcgaacgag tccccgcggt
    33541 tcaggccgag gatgaccatc aggacgaatg caatgccggc catgccggcg atgataatgg
    33601 tcagcctgtc gagctgcttc tgcagcggcg tcttatcgga cctggtcctg ttgaggaggt
    33661 cagcgatgtg gcccatctcg gtttccatgc cggtggcagt cacgatcatc tcgccacgcc
    33721 cgcgggtcac cgaggtgttc atgaaggcca tgttatggcg gtcgcccagg ggcacttccg
    33781 gccttgggat cgactcagtg ttctttggcg aggcgacact ctcaccggtc agggcggctt
    33841 cctcaatctc gaggttcgct gcgacaaaca agcgcccgtc ggccggtacg cggttgccgg
    33901 cttccatcag cacgatgtcg ccgggcacca gtccttccga ttctatctcg acagcccggc
    33961 cgtcacggcg cacgcgggtg atgttcttca tcatcttctc aagcgctgca aggctcgctt
    34021 cagcctttgc ttcaccacgg agccccagca ctgcgttgaa aaccgtcagg cccagcagta
    34081 ccacggtcgt gcctgcctcc cgcgtgatta ccagactgag cagggcagcg ccgaccagga
    34141 tgatctgcat gaagtcccgg tactggcgca ggaatgcata ccagccaggc tcttttctcc
    34201 ttgctttcag aatgttgggg ccatacttct gctggcgctg gtgcacttcg tcagcactca
    34261 gcccttttcc tggatctacg ccgagctgct gtgcaattgc ctctggcggc actgagtacc
    34321 atttcggagg ttgctggatc ttgtcagcca ttctgtgtac tcccagtcaa tacttctcag
    34381 gaatccagcg gaagggatgg tccggctcca cgtagtcgcc cttcggctgg cgtttgggga
    34441 gtttcacctt ctcacggggg atctccttgt aggggatcag gctcagcagg tgggcaatac
    34501 agttcagtcg tccgcgacgc tggtcatgcg aatccacgac aaaccagggc gattcggggg
    34561 tatccgtggc cgcaaacatg gcgtcacggg cccgcgaata gtcgtaccag cggcgatggc
    34621 tctcgaggtc cattgggctg agcttccaga tcttgcggta gtcggttatg cgttcctcga
    34681 agcgctgggt ctggtcctcc atggtctctt ccagccagta ctttacgaga atgatcccgg
    34741 acgcaatcat cgcgtgttcg accgggggca cccagcggag gaagttctcc acctgttcct
    34801 tggtggcaaa tcccatcacc cgctcgacgc ccgcccggtt gtaccagctg cggtcaaaga
    34861 gtaccacttc gccggctgcc ggcaggtgcg ggatgtagcg ctggacatac atctgggact
    34921 tttcccgttc tgtgggcgtt ggcagggcta ccagccggaa gacacgcggg ctggtgcgct
    34981 cggtgatccg cttgatgata ccgcccttgc cggcagcatc acggccttcg aagagcacgc
    35041 agaccttggc gccggttgcc tttacccact cctgcatctt gacgagctca acctggaggt
    35101 tgaccagttc cttctcgaat tccttgcggc tcatcttcgg tttcgattcc ggcgttgcac
    35161 cgggagccgg acggtcagac acagcgggaa ttccgctctg gacttcgtcc tgctgttgac
    35221 gctctgcctt gcgcagtttc ttctttttgc ctttcttttt cgccatagca atactccaat
    35281 gctccatcgg ggtttataaa acttgcatga tcagggtgat aaggtaacct tgcaccatga
    35341 aacctcatca tccccgctgg gggaggaggg acagaaccac cagccggaag ggtattggcg
    35401 aaaaaaaggc aagggggcca gttcattcca gactcctgag aaaaccgggc tatgagaagg
    35461 tgctcctgtt tgaaggtggg tatataaaca taacctgagg gattggaagt tatagtataa
    35521 ttcaagtggc aggaatcgaa cctgcgaact cctgcgagaa ccgatcttga gtcgttcacc
    35581 tttggcctct tggttacact cgctcagaag gattgccgaa aggaaatctt acgctatgat
    35641 tctcaatggg cttgagaaag gtccctgcac ctggttgaga acctgactgc agaaattttt
    35701 accagtgaca gttcctcaaa aacggatccc agtgcttgaa tcatcacgac cgatgactac
    35761 cctagcccaa atgagaactc ctcttccagg ataggtctta tatagaatat tccatat
//
LOCUS       DAOQYR010000015        34991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_1429_length_34991_cov_8.398014, whole genome shotgun sequence.
ACCESSION   DAOQYR010000015 DAOQYR010000000
VERSION     DAOQYR010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 34991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34991
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1429_length_34991_cov_8.398014"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="PKM87_03350"
     CDS             complement(<1..1333)
                     /locus_tag="PKM87_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNQ29135.1"
                     /translation="MSGKDTSPPKAGPAPTKVLGIFALAMINVAAVLSIRNFPSMAEF
                     GWSCIGWYLLGTVMFLIPLSLAGAELATMLSDQGGGVYAWCREAFGEKGGFVAIFCEW
                     SNNLVWFPTVLAFIASTFAFALSPSLGDSPWYLFVVMMIAFWGTTAIACFGEDISTKF
                     QNAGVILGSIIPSVLIIMLGVWWFASGATIVLPPFSPDQVIPEVSLSTLPFFATVILL
                     FAGMEMAGFHALETRNPGADYPKAMALSALIIFGCTVLATLAIAFVIPADELNLASGV
                     MQAIQYFFNAARIPGAVAPMALLIAIGGVVSLAAWLIGPAKGLGVVAEEGNLPPIFNR
                     TNRYGSPVAVLIVQALIGTLISLLYVFLPSVNQAYWILSAITVELLCIVYFLVFAALI
                     RLRYTRPDAPRPFRIPGGTAGAWLIGGMGAGGVIFSFFVGLMPTGDFSATRA"
     gene            complement(1330..3672)
                     /locus_tag="PKM87_03355"
     CDS             complement(1330..3672)
                     /locus_tag="PKM87_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HNQ29136.1"
                     /translation="MLTGKRVGGAVLNLEERLNVVIIDDSIQTDTPRGRTISRIAKGL
                     ADYQIAVTTMASIEDARSAYARLPEADCIMVNWNLGGTGAKDHNDARRLITEIRKRNE
                     DIPIFVIAEPVSGATASMTVDVIREVNEFVNIMDDTPEFIAGRITAAAHRYKESLLPP
                     FFGAMVKFSKDFEYSWHTPGHAGGTAFRKSPAGRLFYNFFGEQLFRSDLSISVGELGS
                     LLDHSGPLGEAERYAAKVFGADETYFVTNGTSTANKIVFFGRVTKDDIVLVDRNCHKS
                     AEHALTMTHSLAVFMVPTRNRYGIIGPIPPGEMTPAAIRAKITACPFVEDSRAAAPVH
                     AIITNSTYDGLCYHAAQVEGLLEKSVDSIHFDEAWYGYARFNPLYRDRFAMRDEARDP
                     NGPTVFATQSTHKLLAALSQASMIHIRNGRVPIEHARFNEGFMMHSSTSPLYPIMASL
                     DVSSKMMEGAAGRVLTTESIEEAIRFRLTMARIHREISKSGKRKDWWFGMWQPDTVND
                     PKARKKIPFADAPLEMLRDNPDAWVLNPGDGWHGFSGLPDNYCMLDPIKVTVVMPGVN
                     EDGSLGSWGIPAAVVTKFLDTRGIVNEKSGDYVILFLFSMGNTKGKWGTLITELFEFK
                     RHYDEKTPIEEIFPELVNEYPERYSGMTLRSLADEMHSFKRERKMCELLEEAYSTLPE
                     PAVAYAEAYRRIVRGEVEKVPVGKAGGRVVATGIYPYPPGIPILAPGERAGPGEGPIL
                     QYLSCLQDFDARFPGFEHDIHGVENVRGEYMMYCIREESA"
     gene            complement(4058..4645)
                     /locus_tag="PKM87_03360"
     CDS             complement(4058..4645)
                     /locus_tag="PKM87_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00296 family protein"
                     /protein_id="HNQ29137.1"
                     /translation="MDALTSEEGTLAVRLARSVLEHAIGGMPEAAIPLPPVFREKRGV
                     FVTLTKKGELRGCIGFPQPHLPLQQAVREAAYAAAREDPRFPPVQARELPGIRVEVTV
                     LSVPMLLTVAPKDRTEAVIVGRHGLIVRGYGRSGLLLPQVPVEWNWDAREFLDHTCMK
                     AGLPAGCWKERGVEVYTFEGQIYSETPPDTGRNTG"
     gene            4680..5031
                     /gene="rnpB"
                     /locus_tag="PKM87_03365"
     ncRNA           4680..5031
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKM87_03365"
                     /product="RNase P RNA component"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00373"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00373"
     gene            5095..5655
                     /locus_tag="PKM87_03370"
     CDS             5095..5655
                     /locus_tag="PKM87_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNQ29138.1"
                     /translation="MHIPTPEEIKARRELLGMKQAELAERAGISQSMVARIESGSVDP
                     RASTLAKIVHVLNIMARPRTTAARVMHTPVISVGVEDTITHAVDIMEKYNISQVPVLE
                     NGVPVGCISESAIVAAIEEQGGHRTQGEQVKHYMESGFPTVPHDMDIETVIRILQHHH
                     AVLVMEEGRVRGVITKHDLIPLIMDR"
     gene            complement(5735..6208)
                     /locus_tag="PKM87_03375"
     CDS             complement(5735..6208)
                     /locus_tag="PKM87_03375"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNQ29139.1"
                     /translation="MGTFICTRCGKCCISLGRHIRIERSLSSVQHYVRNAISGEVTLV
                     TIHPERRALFSEKPDEGWCPFLRREQDGRFTCTIYENRPAICRNFRCLNMVISSQDGT
                     EVGQIVGKATITTKDPVLKELWNNLKKQMPMSDPRWFEYMQKELKMHGYTLEPVT"
     gene            6327..6542
                     /locus_tag="PKM87_03380"
     CDS             6327..6542
                     /locus_tag="PKM87_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29140.1"
                     /translation="MPVEGVLSSPGMSACRVGTSRAATAHGVRGFAQQQGVRIPPILH
                     DMDIETGLHDTPAPPQGAGDEGGGPVT"
     gene            complement(6581..7249)
                     /gene="tpiA"
                     /locus_tag="PKM87_03385"
     CDS             complement(6581..7249)
                     /gene="tpiA"
                     /locus_tag="PKM87_03385"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNQ29141.1"
                     /translation="MGTPFILLNLKTYSEGSGQRAHHIAEAAQQVAEESGVEIGIAPS
                     YMNIHPMSMHYSLPVYAQHVDAAAPGAHTGSVTVEGIRSAGAVGSLVNHSEHRLTLAD
                     IDAAVSALRSAGLISVVCSNNVATSAAAASLGPDYVAIEPPELIGGTVSVSQANPEII
                     TGTVTAVRNVNPSVRILTGAGIHSGDCVKVAVELGTAGVLLASSVVKAKAPISVLRDL
                     VSKL"
     gene            complement(7292..9913)
                     /locus_tag="PKM87_03390"
     CDS             complement(7292..9913)
                     /locus_tag="PKM87_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNQ29142.1"
                     /translation="MPSRSSPHTSVRCPELLAPAGSKEALHAAIAAGADAVYLGGRKF
                     GARHFAANFSEDEIREAVAHAHLRGVLIYVTVNTLVQDGELPDVLAYLLSLYSMGIDA
                     VLIQDGGLLALARGMLPGLSLHASTQCTISTSEGVAWAGKAGFSRVVLARETPLTEID
                     RIMAIPPGERPGIEVFVHGALCYSYSGQCLLSSAIGGRSGNRGMCAQPCRKPYCLVRG
                     VPDRYGRLAGAEEVACPDRFLLSTRDLCCYPGLREIVRRGVESLKIEGRMRSAEYVAA
                     VVRAYRAALDALREGKEWRSTADMEEMATAFNRGFTGGYLLGERGPALMGRDRQDNRG
                     LLLGCVLASPAKGGILVRTQAGYVPAAGDGILIADPGGAVRSGYALNRDAVQHGDALF
                     LPGVGGGAKPGSLVFLTRSMRLSARLKETLHAGSRRSPIPATLRVRIAEGLPLEGTAT
                     CPGPRGGRTEAKCTTGFIPPAIKNAPFSKEAARRQLRKSGGTPFAVEAVDLDCRGDPF
                     VPLGELNRLRRDILSSLQEAWLREFRPEERLIDEAAARVQRFSQEHATKIAQVERMKK
                     GDSIPSLTVYCATREEIAAACEGGCDSVCYEPSTEEWAGYSGELAEGARYCREQRVSF
                     TWKWPRITGPRFLKEALPLVPGLFRDGLARVMVEEPGIAEAILSVEPGMEVIGGQGLN
                     IYNAYAARAFHPPISSFTLSPELSGAQAAELIAISDRVTPGIQYEIFCQGSLEVIVSE
                     DGLISTLAGSRKRKDNVAFGLRDETGRLFPVYEDMMGRTRILNAVETTLIDRIPELLA
                     MGIHSIAVDARGRGERYAGEMTRLYREGLGAAAGDGCSRDAMESLKDRARMMARGGLT
                     SRHFDRGLAGYCDNEAE"
     gene            9976..10611
                     /locus_tag="PKM87_03395"
     CDS             9976..10611
                     /locus_tag="PKM87_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HNQ29143.1"
                     /translation="MRCAVSVPDLAHLAFALSAGTDIIEIRLDLTGNIPEQTSHEVFS
                     ELPVPLIFTLRSTAEGGSFAGSPGKWWENLRPFLPYATYIDVEERFAQFAPRIREMGK
                     TVISSLHTGTMPDPHALVSLEARLRSFGDIPKIVVAPRTEEDVLALLSFTHRAERPII
                     TSIMGTGFRYARLLLPLFGSYLVFCHCGTAASPGQYHIREWREFCRLVRDH"
     gene            complement(10598..11014)
                     /locus_tag="PKM87_03400"
     CDS             complement(10598..11014)
                     /locus_tag="PKM87_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29144.1"
                     /translation="MRETRWRYSLLLALMALATLLSGGCLSVLSQSGPDSVPAAAPGS
                     GLRAALVDQEADWGFSRGCTWTAELQVANPGNTSEKNVHLQVELVNARTGAVRDAKDL
                     FIGAIGPGEERTVRVVLDGECLDEYTVRAIPVVSGP"
     gene            complement(11120..12124)
                     /locus_tag="PKM87_03405"
     CDS             complement(11120..12124)
                     /locus_tag="PKM87_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNQ29145.1"
                     /translation="MNTFGRSFRITTFGESHGPALGVVIDGCPPGIALSEGDIQPFLD
                     RRRPGRSPHSSPRREEDRVEILSGVFEGRTTGAPIALLFRNSDSRPGDYGSLREVFRP
                     GHADYTWWRKYGIRDHRGGGRSSGRETVARVAAGAVAAKVLEQKGIRIGSRVLEIHGR
                     TSPEEMEAAIADAIQAGDSVGGVVEVTAGGCPPGLGDPVFGKLDAMIGLAMLSIGAVK
                     GVEIGDGFAAARVRGSEQNDQMEREGFLTNHAGGILGGISTGAEIVVRIAVKPTPSIA
                     KEQRTVDNAGEERVISVKGRHDPCIVPRLVPVAEAMLALVLADALMEQEKIGTWVTGG
                     "
     gene            complement(12121..13506)
                     /gene="aroE"
                     /locus_tag="PKM87_03410"
     CDS             complement(12121..13506)
                     /gene="aroE"
                     /locus_tag="PKM87_03410"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNQ29146.1"
                     /translation="MNVVLTGFRGTGKTESGRLLARLLDVPFYDTDALVEEEAGATVH
                     EIFEQEGEEGFRAREKRAIAGLPRGPGVFATGGGAVIDPENVEHLRRGRTVVLLNADE
                     STIEKRIQHSTRPALTRMGLRAEIHALMESRRDVYLAAADYCIDTSARSANEVAIGIK
                     RLLAEGVTKESGRREALRAFAGSGIHQSEAAELATMVKALSENPVLRFYGIAGYPSLH
                     SRSPPLFRRLFSYFDINAYYTRFHDPALTRILGTARDLGVRGLSVTLPFKQEIMGHLD
                     RMDAHCRAIGACNTVLFCGGEAHGYNTDWLGIRDPLASLRGSRAVVLGAGGAAAAAAY
                     ALRALEMEVTLLNRTVERAEALAERFGCHHGSLRDFGSIRPDVVVNATPVGMESDTAS
                     PLKKSDLAPGMTVFDLVYTPPETPLIRIARSAGCTAITGKEMFIRQARAQFRQFTGID
                     APLEMIREFLG"
     gene            complement(13503..14771)
                     /gene="aroA"
                     /locus_tag="PKM87_03415"
     CDS             complement(13503..14771)
                     /gene="aroA"
                     /locus_tag="PKM87_03415"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNQ29147.1"
                     /translation="MEVTLQRAGRVDLSFQAPPSKSFTHRALIAAALAEGDSVIQNPL
                     DAEDTRVTRTALIGMGVRVAAVDRLCPVAGTGGALSCPRGLVLDMQNSGTSMRLLTSV
                     ALLCRSHVILTGSARMKERPLGPLAAALTALGGSVSFLEKEGFPPLDVSGSLRGGTAR
                     IDAGMSSQFVSSILMAAPCAMQDVTLELEPGVASPSYLDVTTAVMEAFGARVEREEYR
                     RFHVPAARSYLGRTYQVEGDYSSASYFFAIAALCRGKVSVSNLNPSSCQGDRRFLSAL
                     ESMGCRVRSTEDTVEVSCDNDLDGIEIDMSSSPDTVQTLCMVAANAKSPTRITGIAHL
                     KWKESDRLETTTGLLRSLGGDARVEEDAILIRPAPLHGGMIDPGDDHRTAMSFAVLGL
                     ATGNVKITHAECVSKSFPGFWDQLGGQGLV"
     gene            complement(14762..15559)
                     /locus_tag="PKM87_03420"
     CDS             complement(14762..15559)
                     /locus_tag="PKM87_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HNQ29148.1"
                     /translation="MKVFVLGPEGTFSHELGWRVFGDRVTLVPTIRRIFEAVEAGEGT
                     GLVPLENSEAGGVGPSLDGLLRHNVWITGDLYMPVHHYLVSMGPVGEAEVLFVHPQTH
                     EQCSELVDALEIPVVHTSSNAQSAREMQQSGRGAAIVSQMTARLHNLPIVRERVENNP
                     RNITRFVTLSSEPYHGSDAGKGSIIIDPREDRAGLLYDILGIFARKGINLSRIESRPS
                     KRGIGSYVFFLDFKYNGNADEAVRELEMMTAVKDLGRYPELEVPEWR"
     gene            complement(15556..16380)
                     /locus_tag="PKM87_03425"
     CDS             complement(15556..16380)
                     /locus_tag="PKM87_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNQ29149.1"
                     /translation="MKIGIIGGTGKMGAFFREVFENAGWVVHVSGRSTGLRAADIARV
                     CDVVMVSVPIRETVGMIRSIAPLLSEEQVFCDLTSLKSEPVEAMLASRARVVGLHPMF
                     GPSAGGLSGQTIIATPARCDPATLESLLSVFRSQGARVTLATPVEHDRIMAVVQGLTH
                     FVTLGVAETMRRVGMSPADTLAFMSPVYQMEMGLVGRLLSQDPQLYADILELNPHVPP
                     VLEACEEALSLLRRIVEAGDTGAFEEVFRENSKNFGEYCQRAARESDMLISAMVRE"
     gene            complement(16387..17373)
                     /locus_tag="PKM87_03430"
     CDS             complement(16387..17373)
                     /locus_tag="PKM87_03430"
                     /EC_number="1.4.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase II"
                     /protein_id="HNQ29150.1"
                     /translation="MKEFWVDARPWKKEVVTAALESGADTIVAEESGKVRALGRVRVV
                     APDGDLAIGRDVWEVRIAGKESEENAAQLAKSGYVIVSAEDWTVIPLENLVAQSDRII
                     AAVHDAGEAELALQVLEKGVAGVLLQTGDPKRVKETGAVVHRGQERCELVPFTVTAIR
                     PAGMGDRVCVDTCSMLKEGEGMLVGNTSSAFLLVHAETMENPYVAPRPFRVNAGAVHA
                     YVLSPGGKTAYLSDLAAGDRVLVTGTGGETREVTVGRVKIEERPLLLVEAEADGRPYS
                     LVLQNAETIRLVDEDGRARSVVEIAPGDRVLGCIKESGRHFGMAVKEKILEK"
     gene            complement(17370..18155)
                     /locus_tag="PKM87_03435"
     CDS             complement(17370..18155)
                     /locus_tag="PKM87_03435"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HNQ29151.1"
                     /translation="MIGKEIRIERIMDRNTGRSVVVPMDHGFTLGQIDGLLDMTTTIS
                     EVSEGGANAIVLHKGIVKRGHRRRGRDIGLVVHLSGSTSLNPDPNDKVLVCTVEEAVA
                     LGADAVSIHINLGAPNESRMLESAGKVVKDCNRWGIPLLVMIYPRGKGIDPTSPATVG
                     HCVRVAEELGADLIKTNYTGDPVSFKKIVTSCSVPVFIAGGEKAGDLETLKIIRDSVK
                     AGGAGVCVGRNAFQRENTSAFVRALCRVVHEEMDPEQALMEMG"
     gene            complement(18155..18952)
                     /locus_tag="PKM87_03440"
     CDS             complement(18155..18952)
                     /locus_tag="PKM87_03440"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HNQ29152.1"
                     /translation="MRGKEIRLERIMDRNTGKTIIVPMDHGVTNGPIEGLIDLGRAVD
                     LVATGGANAVLGHVGLSLYGHRKSGRDVGLILHLSASTSIGPDPNEKVLVNSVTSALK
                     MGADAVSIHVNIGADSEARMLSDLGRVAIDCMEWGMPLLAMMYPRGRKIKDEHQVEHV
                     CLAARVAAELGADLVKTVYTGDPDSFRQVTKGCPVPVVVAGGSKTSDLATLEMIEGAM
                     EGGAAGISIGRNAFQHPAPDRFVSAAAEIVHRGCPAEEALEILKEKR"
     gene            complement(19225..19743)
                     /locus_tag="PKM87_03445"
     CDS             complement(19225..19743)
                     /locus_tag="PKM87_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein bridging factor aMBF1"
                     /protein_id="HNQ29153.1"
                     /translation="MSIKMFATSMQCELCGAKIVGPPKTVRVEGAELMVCASCARYGT
                     EVQSPARSSPRAGAAPRAGRTVPARRSRDVFDLIEGEIVEDYGERIRDARMEKGLSQK
                     DLAMQLKEKELLIKKIEKGDLIPEDEVRRKLEKALDIRLIDSPSEEEGKRGGGKVVPT
                     LGDVISIKKVHK"
     gene            19775..21013
                     /locus_tag="PKM87_03450"
     CDS             19775..21013
                     /locus_tag="PKM87_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-activating nucleotidase"
                     /protein_id="HNQ29154.1"
                     /translation="MVESARQPQEPQNPEELYRYLLDRVTNMETRNLELREQIRQIES
                     DKRYIETQKIRYEREVRKLKSEIEQLRSPPLVIGTVTDVLDNGRVVVRSSAGPRFMVR
                     SSMCIDPEKLKPGARCTLNQQSLAVIDVLPESYDAQVYGMEVVESPDETYADIGGLNP
                     QINEVREAVELPLKRPDLFDRIGIEPPKGVLLHGPPGTGKTLLARAVAHETNAHFLRV
                     VGSELVQKYIGEGARLVRELFDLARKKAPTIIFIDEIDAVGASRTEATTSGDREVQRT
                     LMQLLAGMDGFERRGDVKIIGATNRIDILDRALLRPGRFDRIIEIPLPDIAGRVAILD
                     VHTRRMRLEESVDLSELASLTEGKNGADIKAICMEAGMFAIRRDRDAVSKEDFLDAIE
                     KIGLDFDRHVCKSGYGAMFA"
     gene            21312..21611
                     /locus_tag="PKM87_03455"
     CDS             21312..21611
                     /locus_tag="PKM87_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HNQ29155.1"
                     /translation="MVKEFMDFLYEYKVIGLAIAFIMGLAANQLVKSFVDNIIMPVIA
                     VLIPGGDWQTSKLVLGPIVISWGQFLADLIYFIIVAFVIFLIAKKVLREDKVTKK"
     gene            complement(21598..22098)
                     /locus_tag="PKM87_03460"
     CDS             complement(21598..22098)
                     /locus_tag="PKM87_03460"
                     /inference="COORDINATES: protein motif:HMM:NF039541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5804 family protein"
                     /protein_id="HNQ29156.1"
                     /translation="MLVLFVPKEAVALYRELLSSETSRDALRFYRPVQTPAGVQISMA
                     SLGSALSLASDLRWYVRRYMRDVLFEISDGVYCTRPLAQEIYYGRLAVLHTPWKFRKT
                     YTFESGQVIREDPLIVDVGRVQATARSAGGDVAVLEVWCTEEEFLGKKWSEEKSDDVE
                     DDITSS"
     gene            complement(22236..23399)
                     /locus_tag="PKM87_03465"
     CDS             complement(22236..23399)
                     /locus_tag="PKM87_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-activating nucleotidase"
                     /protein_id="HNQ29157.1"
                     /translation="MEESVIRNAGPDAELKYQLKLQDLESQVLDLKVKNDEVLRENAQ
                     LKRENNQLKRMPLFVAVVVDLLDRGEVYLRQQGNNQEYLTHVNDEIFRQLKPGTKVAV
                     NNALSIVKIVGNIFDSRVRVMELDESPEITFDQVGGLAKEIEEVREAVEYPLTKPEIY
                     VRVGVEPPKGILLYGSPGTGKTLIAKAVAHQAKATFIRMSGSELVHKYIGEGAQLVRE
                     LFSLAREKSPSIVFIDEIDAVGSTRTNDGTSGSAEVQRTLMQLLAEMDGFDTRGDVRI
                     MAATNRVDMLDPALLRPGRFDRVISIPLPNPEGRKEILKIHSSRMVLGKDVNLEAIVE
                     MTENATGADLQAVCREAGMMAVRREARTISHDDFVRAVRKVRAEVSTDMRMYT"
     gene            23444..23866
                     /locus_tag="PKM87_03470"
     CDS             23444..23866
                     /locus_tag="PKM87_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HNQ29158.1"
                     /translation="MLIRGRGISKGNGRGRVLTSPEPISFLSGVDPETGMIVEKGHPL
                     EGALITGTVLVFPHGKGSTVGSYIVYALSRNGKAPAAMINLEAEPIIAVGAIIAGIPL
                     VDRLEGGLSLVPDGAIARVDGGEGTVEILEEEAPGETA"
     gene            23893..24327
                     /locus_tag="PKM87_03475"
     CDS             23893..24327
                     /locus_tag="PKM87_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29159.1"
                     /translation="MRLLSLLCMMLLISGVAICGCTQSPAGSAQSPQGTSRTGMIPGP
                     TVTLPPEHAVEIQINEKDPIYATISVIFAGGKGQVMVTDILVRVTRSDGQVTEKHLEA
                     VKGAELKFDGTKEDDRIEAFVSLTNGNTYKVIDQLVPYRTRG"
     gene            24389..25489
                     /locus_tag="PKM87_03480"
     CDS             24389..25489
                     /locus_tag="PKM87_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04013 family B12-binding domain/radical SAM
                     domain-containing protein"
                     /protein_id="HNQ29160.1"
                     /translation="MDVHWRFIPAARNSYAALYAACEQEGYILEPVRTPAPDVTCYSL
                     NSLTASRYLPEIRDAPCITIVGGPHATARTEEMARIADYVVVGEGEFVLPALLRSLES
                     GGPVPPGVATKEGFSPAPHCVRLDAYPPFSTMKGYIEISRGCPHACAYCQTPRIFGPH
                     MRHRSIDAVTHAARRFRDARFVTPNALAYGSDGRTPRLEKVESLLSSLRHEEQHIYFG
                     TFPSEVRPEWVTREAIDLITTYCSNTRLHFGAQSGSDRVLSSLHRGHTVQDVISAVEL
                     CLDAGLTPVVDFIVGIPGEEEDDQRMTARLVEWISRRGRVHLHRFIPLPGTPMEKTAP
                     QPLLPEVERLLGALALRGRVTGSWGDPTRRFF"
     gene            25514..26155
                     /locus_tag="PKM87_03485"
     CDS             25514..26155
                     /locus_tag="PKM87_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNQ29161.1"
                     /translation="MMDVLLLADLHGEFGKLESFLELSPDAIFIAGDITNMGPADAVE
                     SLFSRIDVPSFAVPGNCDPREIIDVLEHSDCVCLHGSSLNLGKISIVGIGGSNPTPFN
                     TPFEMTDKQIDDLLTALVRKMERATHNVLISHAPPQGFLDEAGGNHVGSASIRKHMGS
                     FDLVCCAHIHEQRGVVEEKGVKVVNPGMAALGQCAMVHFGTEPKEIRIELLQV"
     gene            complement(26361..26903)
                     /gene="cyaB"
                     /locus_tag="PKM87_03490"
     CDS             complement(26361..26903)
                     /gene="cyaB"
                     /locus_tag="PKM87_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HNQ29162.1"
                     /translation="MLEIELKVRVDDLAAVRERLCEQQAELESRVMERDLYLNAPHRD
                     FGVTDEALRIRQAGERWTVTYKGKKEPGFHLKAREELNCRVESGDVMARIFISLGFTP
                     VAEVRKWREYYHFKDATVCLDQVEGLGEFVEIELNNPGNVERPAEYVMALGEEIGVTG
                     KPILSSYLEILLAKGQGTEE"
     gene            27166..27858
                     /locus_tag="PKM87_03495"
     CDS             27166..27858
                     /locus_tag="PKM87_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNQ29163.1"
                     /translation="MAIKVGDFIRISYTGRVDGKVFDTTDEDIAKDAGIFNPEASYGP
                     VTIRVGSHHVILGLDEALDGKEIGEEGEVDVPPEKAFGPHEPTQVEAFNKNSFKDKPK
                     KGMSIKVPEKGEGTVVDVIGNRVLIDFNHPLAGKPLSYSFRVEDIVEGAVEQMKGLIK
                     LYAGRDMDVSLNEGALTLHLPPGITYDRRWLLWRSRVIHEGFEHIPTIDEITLVETFA
                     RPEKAEAETPEE"
     gene            complement(28080..29429)
                     /gene="purB"
                     /locus_tag="PKM87_03500"
     CDS             complement(28080..29429)
                     /gene="purB"
                     /locus_tag="PKM87_03500"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNQ29164.1"
                     /translation="MAIHPIEFRYGTAEMRAVWGEEYRFSCIVQAEVALARASAAHLF
                     IPAAAAEAIASCAGKATLARAKEIEEEISHDMMAIVKAIAEQCGEGGGYVHYGATSND
                     ILDTATGLQIRAALDILEGNLRRLLAALLKRADETKMLVCAGRTHGQIGVPTTYGLRF
                     AIWASEVARHIERLGQMAPRVEVGQLTGAVGTQAALGERGMEIQATMMEYLGLTAVDV
                     SNQVVSRDRYAEYFMFLANVATTLDKIGIGIRTLQRTEIGEVEEAFGARQVGSSTMPH
                     KRNPIKSEQVCGLSRVVRSAVEPALQNNTLWDERDLTNSSCERVLFPEATILADHILA
                     LMIRVIEGLTIKPERIAQNLSVLQGVNMAEAVMIELTRRGMDRQEAHESMRLASMQAL
                     EKKVPLAKVLSSDEKVMRFLSPDEVGALLDPLHYIGTAPAQVERLIRKLAPLCRVSV"
     gene            29491..31497
                     /locus_tag="PKM87_03505"
     CDS             29491..31497
                     /locus_tag="PKM87_03505"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNQ29165.1"
                     /translation="MDSLDDWFPYADYRPCQREMLEFGLSCAQEGTIGLIDAPTGSGK
                     SSLLAALLAGREKRRVLVTVRTVSQLNTFIRELSLIREKKPGLKVAYLIGKRGMCPLS
                     GEGDVYRRCEGVKGFSTTLMRQRAERGSLVPSRDPYIQQQIRRMDPQRPLLCPHFIGS
                     RVFLQSEGGLRMVPSPALQTKAERVTSQTVWPHQLPEVASGVCPYEMMILAAQKADVI
                     LLNYRHLFDEDIREALYASLALDPADAILLVDEAHNCGDAVTAAQSVDLDQDTLTLAG
                     TELSGLRRAKAGVEALQQVIPRISGFMEGLQNSFETEDWFDPAIFDRMVVKGSLYHSM
                     AGIVDDLEEISGKLREKNIKAGEFRETALERLTRFLQKIAASSKDPASLTLFRKEDGL
                     VSLEVRSIDPGPPLQEIARSHHACLLISGTLSPVEAYARFYFGDLPARTLSLPNAFPK
                     ENRLVVCASDITTAFSMRQNEKNLQLIRAYIEAFSRLDGNLAVYFPSYQILEGFSKDL
                     TTAGRKKKKIFVEPRDAKDAEESLREFLSLPGSGRSGLLLAVCGGKWSEGLDYRGDLL
                     QGAMVIGLPLAPFNQVRRMVIGYFRRRFGDEGEFLCYTLPAINRGQQALGRVLRTPDD
                     RGVLVLGEKRFLEGKVSRGLPEWMRNEMRSCTLPEFEDLVGRWR"
     gene            31488..31931
                     /locus_tag="PKM87_03510"
     CDS             31488..31931
                     /locus_tag="PKM87_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNQ29166.1"
                     /translation="MEMREGSCRFGLWHVHVHWSKDTVRLVRFSTRPLGGDVPLPIRR
                     YCAGFPEGLHELASIALSPEYPFSAIYRRVREIPYGNTETYGEIARDLNTSPRLVGLA
                     MKRNPTPLVIPCHRVVSKTGTGGFSPDPAIKEALLAMERRNTRCL"
     gene            32099..32683
                     /locus_tag="PKM87_03515"
     CDS             32099..32683
                     /locus_tag="PKM87_03515"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNQ29167.1"
                     /translation="MDRSAVILVGGEAKRANGLEKYFFTFGGKTFIERLTDTLAEVVD
                     EIILVAKDPAQCSRFSHLDNVRCVTDIRRGYGPIGGLHAGALAAKGDALFVCACDMPC
                     VSTQVVESLFSLLEGYDAVIPQWATDMIEPLHAVYRRSALLSALESHESLSLRRMVKG
                     IRTRYVDVAGIRELDPELRTFININKIEDLGKLS"
     gene            complement(32678..33370)
                     /locus_tag="PKM87_03520"
     CDS             complement(32678..33370)
                     /locus_tag="PKM87_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29168.1"
                     /translation="MKLLGLSPALAVVLAVCLLFSGCITPPKETPPGPMTQNGGMATT
                     VPTPSPTQEIMDTQYVTPATPFPTGTPATTRPTYSSTPNFTFEPTVYKVIYLNTIDFK
                     YSVSAYSTTVENPPLIIEVCLAPKMVTRNIWYESRYTSREDVYTTQTYVSPASYFEIR
                     VRDKASGDIVEKEGFGKTYSVDTKKVLQVRTSGEYLVEFSGNDIVATVQMRVPAPAGA
                     DVTPAGTLACPS"
     gene            33525..33926
                     /locus_tag="PKM87_03525"
     CDS             33525..33926
                     /locus_tag="PKM87_03525"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ29169.1"
                     /translation="MEKKSQFWKKTDKSLNTADGYVLKADLLAHDQKFEEAVEYYDKA
                     LEIVPKNADVWAFKGITLQGGLGRDEEARACWEKAKALDHDIAMAVDMTKEEPARGED
                     LESIRWSDLHDSCREKLKRMMLKNVDAGTRK"
     gene            complement(34020..34202)
                     /locus_tag="PKM87_03530"
     CDS             complement(34020..34202)
                     /locus_tag="PKM87_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29170.1"
                     /translation="MVDFTAYLVISIVTLCFGLLVSFQVAHIMFVRRLTRLAKRCVDT
                     GTIAPLLVELEIAHKK"
     gene            complement(34228..34416)
                     /locus_tag="PKM87_03535"
     CDS             complement(34228..34416)
                     /locus_tag="PKM87_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29171.1"
                     /translation="MSKAMDDVSVYLVIGVLMFCIGLMAGYLLMTTYYSRRFLSVAKE
                     CERADSIAPLITEMERES"
     gene            complement(34506..>34991)
                     /locus_tag="PKM87_03540"
     CDS             complement(34506..>34991)
                     /locus_tag="PKM87_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29172.1"
                     /translation="ERLIRALQEETAIPEKTRDEAIGPLKEEHAQNFSAFHDFLVNFV
                     SLALQGLHRADISLEFSFTEGVPRCHRALLHVDGHPRDLPVEEGRRLLACLPLTGEDP
                     HPEQSLLRFYEAMEQRFDRDQKGELDRCSLEIRQEIYPGSAFHARLHLPAQVFIEGIS
                     R"
BASE COUNT         6843 a        11000 c        10226 g         6922 t
ORIGIN      
        1 cagccctcgt tgcggagaag tctcccgtag gcatcagccc gacaaaaaaa gagaagatca
       61 ctcctcctgc acccatgccc ccgatgagcc acgcgcccgc ggtccccccc gggattcgga
      121 acgggcgggg tgcatcgggg cgtgtgtaac ggaggcggat gagtgcggca aagacgagga
      181 agtacacgat gcagagcagt tcgacggtaa tcgcggagag tatccagtac gcctggttta
      241 ccgaaggcag gaagacatag agcagggaga tgagagtccc gatcagggcc tggacaatga
      301 gcactgcgac aggcgaaccg tagcggttgg tgcggttgaa gattggcggg aggttccctt
      361 cctctgcaac caccccgagg ccctttgcgg ggccgatcag ccaggcggca agggagacca
      421 ctcccccgat tgcaatcagg agggccatcg gtgcaacggc tccgggaatg cgggcggcat
      481 tgaagaagta ctggatcgcc tgcatcacgc ccgatgcaag gttcagttca tctgccggga
      541 tgacgaaggc gatcgcaagc gttgcaagga ccgtgcaccc gaagatgatc agcgcggaga
      601 gcgccattgc tttcgggtaa tcggcaccag ggttccgcgt ctccagggca tggaacccgg
      661 ccatctccat tccggcaaag aggaggatga cggtcgcaaa gaatgggagg gtggagaggc
      721 tcacttcggg aatcacctgg tcaggggaaa aaggaggcag aacgatcgtt gcgccagacg
      781 cgaaccacca gacgccgagc ataatgatca ggacagacgg gatgatgctc ccgagaatca
      841 ccccggcatt ctggaacttt gttgatatat cctcaccaaa gcaggcgatc gccgtcgtgc
      901 cccagaacgc gatcatcatc accacgaaaa ggtaccatgg gctgtcacca agggagggcg
      961 agagcgcaaa cgcgaaggtc gaggcgatga atgcgagcac cgtcggaaac cagacgaggt
     1021 tgttcgacca ctcgcagaat atggcgacaa aaccgccttt ctcgccgaat gcctccctgc
     1081 accaggcata gacaccacca ccctggtccg agagcatcgt tgcaagctcc gcccccgcga
     1141 gagagagggg gatgaggaac atgactgtcc ctagaaggta ccacccgatg caggaccagc
     1201 cgaactctgc catggaagga aaattcctga tgctcagcac cgccgcgaca ttgatcattg
     1261 cgagggcaaa gatgccaagt acctttgtgg gggccggccc ggccttcggc ggggatgtgt
     1321 ctttcccgct cacgcggatt cctccctgat gcagtacatc atgtattctc ccctcacatt
     1381 ctcgacgccg tggatatcgt gctcgaatcc cgggaaccgt gcatcgaagt cctggaggca
     1441 tgagagatac tggaggatag gcccttctcc cggccctgcc cgttcaccgg gagcgaggat
     1501 gggaatcccc ggcgggtacg gatagatgcc ggtggcgacg acccggcccc ctgccttccc
     1561 cacgggaacc ttttcgacct ccccccgcac gatccgccgg tatgcttccg cgtacgcaac
     1621 ggcaggctcc ggcagggttg aataggcctc ctcgagcagt tcgcacatct ttcgttccct
     1681 cttgaatgaa tgcatctcgt ctgcgaggct cctcaaagtc atccccgaat accgctccgg
     1741 gtattcgttc acgagttctg gaaagatctc ttcgatcggg gtcttttcgt cgtaatggcg
     1801 cttgaactcg aagagctcgg tgatgagtgt cccccacttg cccttggtgt ttcccatcga
     1861 gaagaggaag aggatgacat agtcgccgga cttctcgttc acgatcccgc gcgtgtcaag
     1921 gaactttgtg accaccgccg caggaattcc ccacgagcca agggagccgt cctcattgac
     1981 acccggcatg accaccgtga ctttgatggg gtcgagcatg cagtaattgt cagggagccc
     2041 ggagaacccg tgccacccgt cgccagggtt cagcacccag gcatccgggt tgtcgcggag
     2101 catctcgagg ggtgcatcgg cgaagggtat cttcttcctt gccttcggat cgttcaccgt
     2161 atccggctgc cacatcccga accaccagtc cttgcgcttc ccggacttcg agatctccct
     2221 gtgtatccgc gccatcgtca ggcggaacct gatcgcctcc tcgatcgact cggtggtgag
     2281 gacccggcct gccgcaccct ccatcatctt ggacgagaca tcgagagagg ccatgatggg
     2341 atagagggga gacgtcgagc tgtgcatcat gaacccttcg ttgaaccgcg catgctcgat
     2401 cgggacgcgc ccgttccgga tatggatcat ggacgcctgg gagagcgccg cgagaagctt
     2461 atgggtggac tgagtcgcaa acaccgtcgg gccattggga tcccgggctt cgtcgcgcat
     2521 tgcgaagcgg tccctataca gggggttgaa cctggcgtat ccataccacg cctcgtcgaa
     2581 atgaatgctg tccacactct tttcgagcag gccctcgacc tgcgctgcat ggtagcagag
     2641 tccgtcgtat gtcgagttcg tgatgatggc gtggacaggt gcggcggccc tggagtcttc
     2701 cacgaacggg caggccgtga tctttgcgcg tatcgccgcg ggagtcatct cccccggcgg
     2761 gatggggcct atgatcccgt agcggttccg cgtggggacc atgaagaccg cgagagaatg
     2821 ggtcatcgtg agcgcatgct cagccgactt gtggcagttc ctgtccacga ggacaatatc
     2881 gtcctttgtg acccgcccaa agaagacgat cttgttcgca gtcgaggtcc cgttcgtcac
     2941 gaaataggtc tcgtcagccc caaagacctt tgccgcgtac cgctctgcct ctccgagcgg
     3001 tcccgaatgg tcgagaagcg acccgagctc gcctactgaa atcgagaggt ccgacctgaa
     3061 gagttgctcg ccaaagaagt tgtaaaagag gcgcccggca ggagacttcc tgaaggccgt
     3121 tcccccggcg tggccgggag tgtgccatga gtactcgaag tcctttgaga acttcaccat
     3181 cgcaccaaag aacggcggga ggaggctctc cttgtaacgg tgcgcagccg ccgtgatcct
     3241 tcccgcgatg aattcggggg tatcgtccat gatgttgacg aactcgttga cctcgcggat
     3301 gacatcgact gtcatcgaag cggtggcccc ggataccggc tctgcgatca caaatatggg
     3361 gatgtcctcg tttcgtttcc tgatctcggt gattagtctc ctcgcatcgt tgtggtcttt
     3421 tgcgccggtg ccaccgaggt tccagttcac catgatgcag tcggcctcgg gcaggcgtgc
     3481 atacgcagac ctcgcgtcct cgatggacgc catcgtcgta accgcgatct ggtagtctgc
     3541 aagccctttt gcgatgcgcg agattgtcct gcctcttggg gtatcggttt gtatggaatc
     3601 atcgatgatg acgacattca acctctcttc caggttcaac actgctcctc ccactctctt
     3661 tccggtgagc atgcaggcat tcgccccgtg agagtaggcc accaggcata ttcatgccaa
     3721 cgggaccggc acccatcctg cgggaaccag aggtaccttt tctcctgtgg gaaacgatcc
     3781 cgtgagcata cgagtatacg gacctgcatg gggcaagcgc agccggaggg gtttcatatt
     3841 acctccaggg gacgatgaac ccatcaaccc ggctccactc acttatccaa gccttccgtc
     3901 aaggactatt taaatgttct tcaccatcga ggatgggtca cggacatgcc gaacggatac
     3961 aggacaggtg aggaaaaaaa gggtgtgtga gtggactatt ttgccgtatc cttgcgcctg
     4021 cctggccttt cttctcctct cggaatctcc aggtcattta ccctgtattc ctgccggtgt
     4081 ccggaggtgt ctcggagtag atctgcccct cgaacgtata gacctctacg cccctctcct
     4141 tccagcaccc ggccggcagt cctgccttca tgcaggtatg gtcaaggaac tccctggcgt
     4201 cccaattcca ctcaaccggg acctggggaa ggagaagccc gctccggccg tatccgcgca
     4261 cgatcaaccc atgccgcccg acaatgacag cctccgtccg gtcttttggc gcaacggtca
     4321 gcagcatggg aacggaaagc acggtcacct caaccctgat ccccggcaac tcgcgggcct
     4381 gcacgggcgg aaacctcggg tcctcccgtg cagccgcata tgccgcctcc ctgacggcct
     4441 gctgaagcgg caggtgcggc tgggggaaac cgatgcaacc ccggagttcc ccttttttcg
     4501 tcagggtcac gaagactccc ctcttttccc ggaaaaccgg ggggaggggt atggctgcct
     4561 ccggcattcc gcctatggca tgctcaagga cagagcgggc cagccgcact gcgagggttc
     4621 cctcttctga tgtgagcgcg tccatcaggt gtaaataagg tgccgcccta ttaaataggg
     4681 agagggagag tgcggccgac ggtggcacat ggtgctgctg aggaaagtcc ccccaccagc
     4741 tgagcacgca gccgccacgc aagtgcgggt ggcgagagtc acggcaatgg cacagaaacg
     4801 acacggcctg cccccagcga tgatgcgatc gagccccacc aaaaagggca atgcatcccc
     4861 gcttttcatg ggcgcggatg actcgctgat gcataagggc aggaatcgat gaaacggtga
     4921 atccctgcgg gtgcaagcca aaacagggca tgtaggctgc tcagtgcctg cccgggtatg
     4981 gcgcgtagct gaatgccgca ccgaacagaa gggggcttac tcctcccact ctattcctgt
     5041 atgcctattc tgcagtacat ttatccactc atatgcacca gggtaatacc actgatgcat
     5101 atccccactc ccgaagagat aaaggcaagg cgggaactcc tgggcatgaa acaggctgag
     5161 ctcgcggaga gggcagggat cagccagtcg atggttgcgc ggatcgagtc cgggagcgtc
     5221 gaccccaggg caagcaccct tgcaaagatt gtgcacgtcc tcaacatcat ggcaagacca
     5281 aggaccaccg ccgcacgcgt gatgcacaca ccggtcatca gcgtcggggt ggaggacact
     5341 atcacccatg ctgtggatat catggagaag tacaacatct cccaggtccc ggtgctcgag
     5401 aacggggtgc cggtcgggtg catctctgaa tcggcgatcg tggctgccat cgaggagcag
     5461 ggaggtcacc gtacccaggg cgagcaggtg aagcactaca tggagtcagg gttccccacc
     5521 gtcccccacg acatggacat tgagacagtg atacggatcc tccagcacca tcacgcggtg
     5581 ctggtgatgg aagaggggcg ggtccgcggc gtcatcacca agcacgatct catccccctg
     5641 atcatggacc gctagagctt cgggacgagg tcagggaaca tgctgatggg agccttggcc
     5701 gtgactatac ctgatgccag agcacccatg cggttcaggt cacaggttcg agggtgtagc
     5761 cgtgcatctt cagttctttc tgcatgtact caaaccatct cggatcgctc atcggcatct
     5821 gttttttcag attgttccaa agttctttca gtacagggtc cttggtagtg atggtggctt
     5881 ttcccacgat ttgtcccact tccgttccat cctggctgga aatgaccatg ttgaggcacc
     5941 ggaaattccg gcagatggca ggccggttct cgtaaatggt gcaggtgaag cgcccgtcct
     6001 gttctctccg caggaacggg caccacccct catctggctt ttctgaaaag agggcacgcc
     6061 tttccggatg gatggtcaca agggtaacct ctcccgagat cgcattccgg acgtaatgct
     6121 gcactgatga gaggctccgc tcgatccgga tatgtcgacc aaggctgatg cagcacttcc
     6181 cgcaccgggt gcagataaat gttcccatgc ctacctgggg ttttcctggg aaatatcata
     6241 aaggtgcata aaaggacatc cccgtgagag aatctttggc cgccctgctc ttctccccat
     6301 ggtatgagtg aggagtatca acattgatgc cagtcgaagg tgttctgtct tcacccggga
     6361 tgagcgcctg ccgggtggga acttcaaggg cggccaccgc acacggggtg agaggctttg
     6421 cgcaacaaca gggagtcagg attcccccca tcctccacga catggacatc gaaacgggat
     6481 tacatgatac tcctgcaccg ccacaaggtg caggtgatga ggggggcggg cccgtgacgt
     6541 aatcaccacg caatgacctc atccccctga tcagggaccg ctagagtttt gaaacgagat
     6601 cccggagcac gctgattggg gcctttgcct tgaccacgct cgatgccagg agaacgcctg
     6661 ctgtgcccag ctcgacggca accttcacgc agtcacctga gtggatcccc gctcccgtca
     6721 ggatcctgac ggaggggttg acgttccgca ccgcggtaac agtccccgtg atgatctcgg
     6781 gattcgcctg ggaaactgag actgtcccgc cgatcagctc ggggggttcg atcgcgacgt
     6841 aatcaggccc gagcgaggca gctgcagcgc tggtggcgac attattcgag cagactaccg
     6901 aaatgagccc tgcggaccgg agcgccgaga ccgccgcatc gatatctgcc agggtgaggc
     6961 ggtgctcgga atggttcacg agggagccaa ccgctcccgc ggacctgatc ccctcgacag
     7021 tcacagagcc tgtgtgggcg ccaggcgcag cagcatccac atgctgggcg tacacgggaa
     7081 gcgagtagtg cattgacatc gggtggatgt tcatgtagct tggggcgatc ccgatctcca
     7141 cgcccgactc ctcggccacc tgctgggccg cttcggcgat gtggtgggca cgctggccgc
     7201 tgccctcgga gtatgtcttc aggttgagaa ggatgaatgg ggttcccata cggatctctc
     7261 agccagtggt atggatcccc caggggaaaa cctattctgc ttcattatca caatacccgg
     7321 ccagcccccg gtcaaagtga cgggaggtta gcccgcccct cgccatcatg cgcgcccggt
     7381 ccttcagact ctccatggcg tccctggaac acccgtcgcc ggccgcggca ccgagcccct
     7441 cgcggtagag gcgggtcatc tcaccggcat aacgctcccc tctcccccgg gcatcgaccg
     7501 cgattgagtg gatgcccatg gccagaagct cgggaatacg atcgatcagg gtggtctcga
     7561 ctgcgttcag aatgcgggtc ctccccatca tgtcctcgta gacaggaaag agtctgcctg
     7621 tctcatcacg gaggccaaat gcgacattat ccttcctttt tctgcttcct gcgagcgtgg
     7681 agataagccc gtcctcgctc acaatcacct caaggctccc ctggcagaaa atttcgtact
     7741 ggatgccagg tgtcacccgg tctgagatcg caatgagctc tgccgcctgt gccccggaga
     7801 gctcgggcga gagggtgaac gaggatatgg ggggatggaa cgcccgtgcg gcgtacgcgt
     7861 tgtagatatt caggccctgc ccaccgatga cctccattcc gggttccact gaaagaatcg
     7921 cttcggcgat gccgggttct tcgaccatca cgcgggccag gccatccctg aaaagcccgg
     7981 gcacgagtgg aagggcctct ttgaggaacc ggggtcccgt gatgcgtggc cacttccagg
     8041 taaacgagac ccgctgttca cggcagtacc tggccccctc ggcgagttcc ccggagtacc
     8101 ctgcccattc ctccgtggaa ggctcgtaac agacagagtc gcatccgcct tcacaggctg
     8161 ctgcaatttc ctcgcgtgtc gcacagtaca cggtcagtga cgggatcgaa tcccctttct
     8221 tcatcctctc cacctgcgcg attttggtag catgttcctg cgagaaccgc tgcacgcgcg
     8281 cggctgcctc gtctatgagc cgttcttccg gccggaactc acggagccat gcctcctgga
     8341 gggaggaaag gatatccctt ctcagccggt tcagctcgcc gagcgggacg aacgggtcac
     8401 cccggcagtc aagatccacc gcctccacgg cgaagggtgt gccgccggac ttccggagtt
     8461 gtctccgggc agcctccttt gaaaaagggg cgttctttat agccgggggg atgaacccgg
     8521 tggtacattt ggcctctgtc ctgccaccgc gcgggccagg acatgtggca gtcccttcca
     8581 gggggagacc ttcggcaatc cggacccgga gtgtcgcggg tataggggac ctccgtgatc
     8641 ccgcatggag ggtctctttc agccttgcgg agagccgcat gctcctggtg agaaagacga
     8701 gggagccagg cttcgcacct ccccccactc cgggcaggaa gagtgcgtcc ccgtgctgca
     8761 cggcatcccg gttgagggcg tacccgctcc gcaccgctcc tccgggatcc gcaatgagga
     8821 tgccgtctcc tgctgccggc acgtagcccg cctgggtgcg gaccaggata cctccctttg
     8881 caggcgatgc caggacacac cccaggagga gtccccggtt gtcctgccgg tcccttccca
     8941 tcagggcagg gccgcgctcc cccaggagat accctcccgt aaaccccctg ttaaaggccg
     9001 tggccatctc ctccatgtcc gcagtggagc gccattcctt cccctcccgc agcgcgtcga
     9061 gggccgcccg gtacgcccgg accactgccg cgacgtactc cgcggaacgc atccggccct
     9121 ctatcttcag ggactcgacc cccctcctga cgatctcgcg gagccccggg tagcagcaga
     9181 ggtcccgcgt ggagaggagg aagcggtcgg ggcaggcgac ctcctccgca cccgcgagcc
     9241 gtccataccg atcgggaacg cctcgcacca ggcagtacgg cttccggcag ggctgtgcgc
     9301 acatgcccct gttcccgctc cttcccccga tcgcagagga gaggaggcac tgcccggaat
     9361 acgagtagca gagtgcccca tggacgaaga cctcgatccc cggtctctct ccggggggaa
     9421 tcgccatgat ccggtctatc tcggtgagcg gtgtctcgcg cgccagcact acgcgggaga
     9481 atcctgcctt cccggcccat gccaccccct cactcgtgga gatggtgcac tgggtcgagg
     9541 cgtgcaggga gagacctggc agcatcccgc gggcgagtgc aaggagccca ccgtcttgta
     9601 tgagcactgc gtcgattccc atcgaataca gcgaaaggag gtaggcaagg acatcgggga
     9661 gttccccgtc ctggacgagg gtgttgaccg tgacatagat gaggacgccg cggaggtgtg
     9721 catgcgcgac cgcctcgcgt atctcgtcct cgctgaagtt ggccgcgaag tgccgtgccc
     9781 cgaacttcct cccccccagg tacaccgcat ccgctccggc ggctatcgcc gcgtgaagtg
     9841 cttccttcga tccggcgggt gccaggagtt cggggcagcg gacagaggta tgaggggatg
     9901 aacgcgaagg cattctcaac aatgagaggt cgcccgtggg caggttaaag actcgcccgg
     9961 gcataaagtg gagtaatgcg gtgtgccgta tcggtcccgg accttgccca tctcgcgttc
    10021 gccctgtccg ccggaacgga tatcattgaa atcaggctgg atctcaccgg aaatataccg
    10081 gaacagacct cacatgaagt cttttcggaa ctccccgtac cgctcatctt cacgctccgg
    10141 agcaccgccg aggggggatc ctttgctggc agccccggga agtggtggga gaatctccgt
    10201 ccgttccttc cctatgcaac ctacatcgac gtggaggagc ggttcgcgca gttcgccccg
    10261 cgcatcaggg agatgggaaa gaccgtgatc tcctcgctcc ataccggcac tatgcctgat
    10321 ccccacgcgc tggtgtcgct ggaggcgaga ctgcgctcct tcggggacat cccgaagatc
    10381 gtcgtcgccc cgcgtacgga ggaggacgtc ctggcactcc tctcctttac ccaccgggcc
    10441 gaaagaccca tcatcaccag tatcatgggg accgggttcc ggtatgcccg gctcctgctc
    10501 ccgctgttcg ggtcgtacct ggtattctgc cactgcggga ccgctgcatc gcccggccag
    10561 taccacatcc gcgagtggag ggagttctgc cggctggtca gggaccactg acgaccggga
    10621 tcgccctcac ggtatactcg tcgaggcact cgccgtccag cacaacgcgg accgtccttt
    10681 cttcgccggg accgatcgcc ccgataaaga ggtcttttgc atccctcacc gctcctgtcc
    10741 tcgcgttcac gagctccacc tggaggtgca cgttcttctc gctcgtgttc ccgggatttg
    10801 cgacctgtaa ctcggcagtc caggtacaac ccctcgagaa tccccagtcg gcctcctggt
    10861 caacgagggc ggcccggagc ccggaaccag gggcggctgc cgggacgcta tccggccctg
    10921 actgtgagag gaccgagaga catccaccgg aaaggagggt cgccaatgcc atgagggcaa
    10981 ggagaagcga atatctccac ctcgtctctc tcaccgggtt cggcccccct tattcaggcg
    11041 tcgtatctgg aatggatgaa agacttgcgt gcatcgcacc ataactgcag gaagggttga
    11101 ccgggcagga taaatgcttt catcccccgg tcacccacgt ccctatcttc tcctgctcca
    11161 tcagggcatc ggcaaggaca agcgcgagca tcgcctcggc gacagggaca aggcggggta
    11221 cgatgcaggg atcgtgcctt ccttttaccg agatcactcg ctcttcccct gcattatcca
    11281 ccgttcgctg ctcctttgcg atggagggag tcggctttac cgctatccgc acgacgatct
    11341 ccgcacccgt gctgatgccg ccgaggatgc cccccgcatg attggtgaga aatccctctc
    11401 tttccatctg gtcgttctgt tcgctgcccc gcaccctcgc cgcggcaaag ccgtccccaa
    11461 tctcgacgcc cttgaccgcg ccgatggaga gcatcgccaa gccgatcatg gcatccagct
    11521 tgccgaacac cgggtccccg agcccaggcg ggcacccgcc agcggtgacc tccacgactc
    11581 cccctaccga gtcccctgcc tggatggcat ccgcgatcgc ggcctccatc tcctcagggg
    11641 aagtgcgacc gtggatctcg aggaccctgc tcccgattcg gatccccttc tgttccagga
    11701 cctttgccgc gaccgcaccc gccgcaacgc gggcgaccgt ctccctgcca gagctcctcc
    11761 cgccaccccg gtggtccctg atgccgtatt tcctccacca cgtataatcc gcatgcccgg
    11821 gcctgaacac ctccctgagc gacccatagt ccccgggccg tgagtcggag ttcctgaaaa
    11881 ggagcgcgat cggtgccccg gtagtcctcc cctcgaagac tcccgagagg atctccacgc
    11941 ggtcctcctc ccggcgggga gaagaatgcg ggctcctgcc aggcctcctc cggtccagga
    12001 acggctggat gtccccctcc gagagggcaa tccccggcgg acacccgtct atgacgacgc
    12061 cgagtgcagg cccatggctc tccccgaatg tcgtaatcct gaaggaacgg ccaaaggtgt
    12121 tcatcccagg aactccctga tcatttcgag cggcgcatcg ataccggtga actggcggaa
    12181 ctgtgccctt gcctggcgta tgaacatctc cttcccggtg atggccgtgc acccggcgct
    12241 ccgggcgatc ctgatcaggg gggtctcggg gggcgtgtag acgaggtcaa agaccgtcat
    12301 cccaggagcg aggtccgact ttttcagcgg gcttgccgtg tcggactcca tccccaccgg
    12361 cgtcgcgttc accaccacgt cgggccgaat gctgccaaag tcacggaggg acccatggtg
    12421 gcagccgaag cgctctgcca acgcctcggc acgctcgaca gtccggttca ggagggtgac
    12481 ctccatttcg agcgcccgca atgcatatgc tgcagcagca gcagcacccc cggcaccgag
    12541 gacgactgcc ctgctcccgc gaagggatgc cagggggtcc cggatcccca accagtcggt
    12601 gttgtagcca tgggcctctc cgccgcagaa gagcacggtg ttgcaggcgc cgattgccct
    12661 gcaatgggcg tccatgcggt cgaggtggcc catgatctcc tgcttgaagg gtaacgtcac
    12721 cgagaggccg cgcacgccaa ggtcccgggc agtcccgagg atacgggtaa gcgcggggtc
    12781 gtggaacctc gtgtaatagg cgttgatatc gaagtaggag aagaggcgcc ggaagagcgg
    12841 ggggctcctg ctgtggaggc ttgggtatcc cgcgatgccg tagaaccgaa ggacaggatt
    12901 ctcggagagt gccttgacca tggttgccag ttccgcggcc tcggactggt ggatgccgga
    12961 acccgcaaac gcgcggagcg cttccctgcg cccactttct ttcgtgacac cctcggcaag
    13021 gaggcgcttg atcccaatcg cgacctcgtt tgcactccgt gcgctggtgt cgatgcagta
    13081 gtcagcggca gcgaggtaca cgtccctcct ggactccatg agggcgtgga tctcggcccg
    13141 caaccccatc ctcgtgagcg cagggcgtgt gctgtgttgg atgcgcttct cgatcgtcga
    13201 ctcgtctgcg ttcaggagga cgaccgtcct cccccttctc aggtgttcca cgttctcggg
    13261 atctatcacc gccccgccgc cggtcgcaaa cacccccggt ccgcggggaa gccccgcaat
    13321 cgcccgcttc tccctggcgc ggaacccttc ttcgccttcc tgttcgaaga tctcgtgcac
    13381 cgtagccccc gcttcctcct cgaccagtgc atcggtatcg taaaagggga catcgagcag
    13441 gcgggccaga agccggcccg actctgtttt ccccgtcccc cggaaccctg tcagcacaac
    13501 gttcatacca gtccctgccc cccgagctga tcccagaaac ccgggaacga tttactgaca
    13561 cattccgcat gcgtaatctt tacgtttcct gtcgcaaggc cgaggaccgc gaaactcatc
    13621 gccgtccggt gatcatcgcc cgggtctatc atcccgccgt gcaggggtgc aggccgaatc
    13681 aagattgcgt cttcttccac cctcgcatcg cctccgaggc tgcgcaggag tcccgttgtc
    13741 gtttccaggc ggtcgctctc cttccacttc aggtgggcga tgccggtaat ccgggtcggg
    13801 cttttcgcgt tcgctgcgac catgcagagg gtctggacgg tgtcgggtga cgaggacata
    13861 tcgatctcga tcccatcgag gtcattgtcg cacgagacct cgactgtgtc ttctgtgctc
    13921 ctcaccctgc accccatcga ctcaagggcg gagaggaacc tcctgtctcc ctggcacgag
    13981 gaggggttca ggttcgagac ggagactttc cccctgcaca gggcggcaat tgcaaagaag
    14041 tacgacgctg atgagtagtc cccctcgacc tggtaagtcc ggcccaggta cgatcgtgcg
    14101 gccggaacat ggaaacgccg gtactcttcc ctctccacac gtgcgccgaa cgcctccatc
    14161 accgcggtgg tcacgtcaag ataagagggg gatgcaaccc caggctcaag ctcgagtgtc
    14221 acatcctgca tcgcacatgg tgctgccatc aggatcgagg agacgaactg gctgctcatc
    14281 ccggcatcga tgcgtgccgt cccgccgcgg aggcttcccg atacgtctaa cggcgggaag
    14341 ccctccttct ccaggaagct gacactcccg ccaagcgcgg tgagcgcggc ggcaaggggc
    14401 ccgagcgggc gttccttcat gcgagcggat cccgtgagga tcacgtgact cctgcacagg
    14461 agcgcaacag aagtgagaag ccgcatgctg gtccctgaat tctgcatatc caggacgagg
    14521 ccgcgcgggc aggagagcgc gccgcccgtg cctgcaaccg ggcacaggcg gtccacggcg
    14581 gcgacccgga cacccatccc gataagcgcc gttctggtca ccctggtgtc ctccgcgtcg
    14641 agcgggttct ggataaccga gtcgccttcc gcgagcgccg cggcgatcag ggcccggtga
    14701 gtgaagctct tcgacggagg ggcctggaaa gagaggtcga ccctcccggc cctctggagc
    14761 gtcacctcca ttcaggaacc tccagctccg ggtacctccc cagatccttg accgccgtca
    14821 tcatctcgag ctcgcgtact gcctcgtctg cattcccgtt atatttgaaa tcgaggaaga
    14881 agacgtaact cccgatgccg cgcttggagg gccgtgactc gattctggag aggttgatac
    14941 ccttccttgc gaatatcccg agaatatcat agaggagtcc tgcacggtcc tcccttggat
    15001 cgatgatgat gctccctttc ccggcatcgc ttccgtggta aggctcggac gagagggtca
    15061 cgaacctcgt gatgttccgg ggattgttct ccacgcgctc gcggactatc ggcaggttgt
    15121 ggaggcgcgc ggtcatctgc gagacgattg ccgccccccg cccggactgc tgcatctccc
    15181 gggcgctctg cgcattgctc gaagtgtgca ccaccgggat ctccagggca tccaccaact
    15241 cgctgcactg ctcgtgggtt tgtgggtgta caaacaggac ttcagcctcc ccgaccggtc
    15301 ccattgagac caggtagtgg tggacgggca tgtagagatc tcccgtgatc cagacattgt
    15361 gccggagcag gccgtcgagg gatgggccga ccccgcccgc ctcgctgttc tcaagtggga
    15421 caagccccgt cccttcgccc gcctccaccg cctcgaaaat tcgcctgatg gtggggacga
    15481 gggtgacccg gtccccgaag acgcgccagc ccagctcgtg gctgaacgtg ccttccgggc
    15541 cgagcacaaa gaccttcatt ccctcaccat ggccgagatc agcatgtccg actcccttgc
    15601 cgccctctgg cagtactcgc caaaattctt tgaattctcc ctgaacacct cctcgaatgc
    15661 ccccgtgtct cccgcctcta cgatccgccg gagcaaggag agcgcctcct cgcaggcctc
    15721 gagcacaggg gggacatggg ggttgagctc caggatgtct gcgtacagct gcgggtcctg
    15781 ggagaggagc cgcccgacga gtcccatctc catctggtag accgggctca tgaacgccag
    15841 ggtatccgca gggctcatcc ccacccgcct catcgtctct gcgaccccga gggtgacgaa
    15901 gtgggtcagg ccttgcacca ccgccatgat gcggtcgtgt tccactggag tcgcaagggt
    15961 gaccctcgca ccctgggacc ggaataccga gaggagggac tcgagcgtag ccgggtcgca
    16021 gcgggctggt gttgcgatga tggtctggcc cgacagcccg cctgccgaag ggccgaacat
    16081 cgggtggagc cccaccaccc gggcccggga agcgagcatg gcctccaccg gctccgactt
    16141 cagcgatgtg aggtcacaga agacctgctc ttctgacagg agcggtgcga tcgagcggat
    16201 catgccgaca gtctccctga tggggaccga gaccatgacc acgtcgcaaa cccgcgcaat
    16261 gtccgctgct cgaagcccgg tactcctccc cgagacatgg accacccatc ccgcgttctc
    16321 gaagacttcc cggaagaacg cccccatctt gcccgtgccg ccgataatcc cgatcttcac
    16381 ctgcgatcac ttctccagga tcttctcttt tacggccatc ccgaagtggc ggccggactc
    16441 cttgatgcac cccagcaccc tgtcgccggg agcgatctcc accacggacc gggcacgacc
    16501 gtcttcatcc accagccgga tggtctcggc gttctggagg acgaggctgt atggcctgcc
    16561 atctgcctcg gcctcgacga ggagaagagg cctctcctca atcttcactc ttccgactgt
    16621 cacctcccta gtctcgcccc cggtcccagt caccaggacc cggtcgcccg cggcaaggtc
    16681 cgagaggtag gcagtctttc cacccggaga gagcacgtac gcatgcaccg ccccggcatt
    16741 gacgcggaac ggccggggtg cgacataggg gttctccatg gtctctgcgt ggacgaggag
    16801 gaatgcagag gacgtgttcc cgacgagcat cccctccccc tccttcagca tggagcaggt
    16861 gtcgacgcag acgcggtctc ccatccccgc cggccggatc gcggtgactg tgaacggcac
    16921 cagttcgcag cgctcctgtc ccctgtggac aacagcccct gtctctttca ctctcttcgg
    16981 gtcaccggtc tggaggagga cccctgccac tcccttctcg agcacctgga gggcaagctc
    17041 cgcctcccct gcgtcatgca cggcggcaat gatccggtcc gactgcgcca cgaggttttc
    17101 gagcgggatg accgtccagt cctccgcgct gacgatgaca tacccgctct tcgcgagctg
    17161 ggcggcgttc tcctcgctct ccttgcccgc aatcctcacc tcccagacgt ccctgccgat
    17221 cgcgaggtcg ccgtctgggg ccaccacgcg gaccctcccg agcgccctca cctttccact
    17281 ctcttcggca acgatggtgt ctgcaccgct ctcgagagcg gcggtcacca cctctttttt
    17341 ccagggccgg gcatccaccc agaactcctt catcccatct ccatgagcgc ctgctccggg
    17401 tccatttcct catggaccac gcggcagagg gccctcacga acgcactggt gttctccctc
    17461 tggaaagcgt ttcgcccgac gcacacgccc gccccgccgg ccttcacaga gtcgcggatg
    17521 atcttgagcg tctcgaggtc tcctgccttc tccccgcccg cgatgaagac cgggaccgag
    17581 caggaggtga cgatcttttt gaaggagacc gggtctcccg tgtagttggt cttgatcagg
    17641 tctgcgccga gctcttctgc taccctgaca cagtggccca cagtcgcggg cgaggtaggg
    17701 tcgatcccct ttccccgggg gtagatcatg acgaggagcg ggattcccca gcggttgcag
    17761 tccttcacca ctttccccgc ggattcgagc atcctcgact cgttcggagc tcccaggttg
    17821 atgtggatgg agactgcgtc tgcgccgagc gcaacagcct cttccacggt gcagacaagc
    17881 accttgtcgt tcgggtcggg gttgagggag gtgctccccg agaggtggac gacaagcccg
    17941 atgtcccgcc ccctcctcct gtgcccgcgc ttgacgatcc ccttgtggag gacgattgcg
    18001 ttcgccccgc cctcgctcac ctcggagatg gtcgtggtca tgtccagaag cccgtcgatc
    18061 tgccccagcg tgaacccgtg gtccatgggg actaccacgg accggcccgt gttccggtcc
    18121 atgatgcgct caatccgaat ctcttttcct atcatcatct cttctccttc agtatctcaa
    18181 gtgcctcttc cgccgggcat cccctgtgga cgatctctgc cgcggcgctg acgaaacggt
    18241 ctggggcggg gtgctggaag gcatttcgcc cgatggagat ccctgcggca cccccttcca
    18301 tcgccccctc gatcatttcc agggtggcaa ggtcgcttgt cttcgagccg ccggccacta
    18361 ccacaggcac cgggcagccc ttcgtcacct ggcggaagct gtccgggtca ccggtgtaga
    18421 ctgtcttcac caggtccgca ccgagctccg ccgccaccct ggcggcaagg cagacgtgct
    18481 ccacctggtg ctcgtccttg atcttcctcc ccctcgggta catcatcgcg aggagcggca
    18541 tcccccactc catgcagtcg atggcaacgc gcccgaggtc cgagagcatc cgtgcctccg
    18601 agtcggcccc gatgttcacg tggatggaga ccgcgtcggc ccccatcttc agggcgctcg
    18661 tcaccgagtt caccagcacc ttctcgttcg ggtcgggccc gatcgaggtg ctggccgaga
    18721 ggtggaggat aagccccacg tccctcccgc tcttcctgtg gccgtacagg gagaggccca
    18781 cgtggccgag gactgcattc gcacccccgg tcgccaccag gtcgaccgct ctcccgaggt
    18841 ctatcagccc ctcgatcggt ccgttcgtga caccatggtc cattgggacg atgatcgtct
    18901 tgccggtgtt ccggtccatg attcgttcca ggcgaatttc ttttcctctc atgtaacact
    18961 cctatccatc cagcgcattt gtgtgagaaa tggcagatgg aatcagtaaa aataccagaa
    19021 ataaccaaaa tagcggtgat agcgggaatt agccgcggct attggtttta cgttcgaaag
    19081 aatggtctca acgatgctcg gtgcggacgc caggctagcg gggataaaaa ccctcaccgg
    19141 cgaaaaaaca ctcaccttgc attgaagaac agaatggaac gaacaagtat ataaatatga
    19201 tgagattgcc gggatacccc ggcactactt atggaccttt tttatggaga tgacgtcccc
    19261 gagcgtgggg actaccttcc cgccgccgcg tttgccttcc tcctcggagg gagagtcgat
    19321 gagccggata tccagtgcct tctcaagttt cctcctcacc tcgtcttccg ggatgagatc
    19381 ccccttctcg atcttcttga tcaggagttc cttctccttg agctgcattg ccaggtcctt
    19441 ctgcgagagc cccttctcca tccgggcatc gcggattctt tcgccgtagt cctccacgat
    19501 ctccccttcg atcaggtcaa agacgtccct cgacctcctg gcaggcaccg tccgacctgc
    19561 acggggtgca gccccggccc ggggcgaaga tcttgcgggg ctctggacct cggttccata
    19621 ccgggcgcaa cttgcgcaca ccatgagttc ggccccttcc acgcgtacag tctttggagg
    19681 gcctacaatc tttgctccgc acaattcgca ctgcatactc gtcgcaaaca tctttataga
    19741 catttttccc tatatactta tttgaggcag aaaaatggtg gaaagcgccc gacaacccca
    19801 ggaaccccag aaccccgagg agctctaccg ctatctcctg gaccgggtca ccaacatgga
    19861 gacccgcaac ctggaactgc gcgagcaaat ccgccagatc gagtcggaca agcgctatat
    19921 cgagacccag aagatacggt acgagcggga agtcaggaag ctgaagagcg agatcgagca
    19981 gttgaggagc cctcccctcg tcatagggac ggtcacggat gtgctggaca acgggagggt
    20041 ggtcgtgcgg agcagtgccg gcccaaggtt catggtcagg agttccatgt gcatcgatcc
    20101 cgagaaactc aaaccgggtg cccggtgcac cctgaaccag cagtccctcg ccgtcatcga
    20161 cgtccttcca gagagttacg acgcccaggt gtacggcatg gaggtggtcg agagccctga
    20221 tgagacatat gccgatatcg ggggtctgaa cccgcagatc aacgaggtga gggaggcggt
    20281 cgagttaccg ctcaagaggc cggatctctt cgaccgcata gggatcgaac ccccgaaggg
    20341 agtgctcctc cacgggcccc cggggacggg aaagaccctc ctcgcgaggg ccgttgccca
    20401 cgagaccaat gcacacttcc tgcgggtggt cggatctgag ctcgtgcaga agtatatcgg
    20461 ggagggagcc cgccttgtcc gggagctctt cgaccttgcg cggaagaagg ccccgactat
    20521 catcttcatc gacgagatcg acgcggtcgg cgcctcgcgg acggaggcca ccacttcagg
    20581 cgatcgcgag gtgcaacgga cgctgatgca gctcctggcc gggatggacg gattcgagcg
    20641 gcgtggcgac gtcaagatca tcggggcgac caaccgtatc gatattctcg accgggcgct
    20701 gctccggccc ggaaggttcg accgcatcat agagatcccc ctccccgaca ttgcagggcg
    20761 ggtcgccata ctcgatgtcc ataccaggag gatgcgcctt gaagagagtg tcgacctctc
    20821 tgaacttgcg tccctgaccg aggggaagaa cggggccgat atcaaggcca tctgcatgga
    20881 agcaggcatg tttgcaatcc ggagggacag ggacgcggtc tcgaaggagg actttctgga
    20941 tgccatagag aagatcgggc ttgacttcga ccggcatgtc tgcaagagcg ggtacggcgc
    21001 aatgttcgcc tgaatatttt tcttctttta gttttttccc ggttccctac cgcttgacgt
    21061 ctggaaggca ttcaaggggg tggcagcaca ctctcctttc cctaaacttc cgaacgccgt
    21121 tttgacgttc atacgttaag tcaactacct atgcctaatc gtgatgaaaa ttgatcgggg
    21181 acctgtttgg atttgaacaa agaaaaatgg aaataatgca cttggaattg taaaaatcga
    21241 aatggggtgc gccatccaag gttttattag ccaccccgtg cttttctcaa ctacaggtgt
    21301 acaaaatgag catggttaaa gaatttatgg acttcctgta cgagtataag gtcatcgggc
    21361 ttgccatcgc atttatcatg ggtttggctg caaaccagct ggtcaagtcc ttcgtggaca
    21421 atatcatcat gccggtcatc gcagtgctca tccctggcgg agactggcag acctcgaaac
    21481 tggtactggg tccgattgtt atcagctggg gacagttcct ggcagacctg atctacttta
    21541 tcatcgtggc gttcgtgata ttcctcatcg caaagaaagt gctccgcgag gacaaggtca
    21601 cgaagaagtg atgtcgtcct ccacgtcatc actcttttcc tcgctccatt ttttccccag
    21661 gaactcctct tcggtgcacc agacttcaag caccgccacg tcccctcctg cagacctggc
    21721 agtcgcctgt accctgccaa catctacgat gaggggatct tcccggatca cctggcccga
    21781 ctcaaacgta taggtcttcc tgaacttcca gggagtatgc aggaccgcaa gcctcccata
    21841 gtatatctcc tgcgccagtg gcctggtgca atacactcca tcggagatct caaagaggac
    21901 atcacgcatg tacctcctca cgtaccaccg gaggtcggac gcgagcgaga gtgcgcttcc
    21961 aagcgacgcc atcgatatct gcacgcctgc aggggtctgg accggccggt aaaaccgcag
    22021 cgcgtccctg ctggtctccg aagataagag ctcccggtag agcgccactg cttccttggg
    22081 gacgaagagc accagcacgg cgatacactc ctgtgcgaac tattggtccc gccaggagtt
    22141 aagatgcagt gctcaggcgc ggtcgccacg agatggcgaa tatctcacat tccggggcag
    22201 acaaggaaaa ttgaatggaa aataggggca cttcttcagg tatacatcct catatcagtg
    22261 ctgacctctg cacggacttt cctgaccgcc ctgacaaaat catcgtgcga gatggtccgt
    22321 gcctcccttc gcactgccat catcccggcc tccctgcaga cggcctggag gtccgcccct
    22381 gtggcgttct ctgtcatctc gacgatcgcc tcgagattta cgtcctttcc aaggaccatt
    22441 cgcgaggagt ggatcttcaa tatctccttc ctcccctctg ggttgggaag gggaatggag
    22501 atgacgcggt cgaacctgcc tggccggagc agcgccgggt caagcatgtc cacccggttg
    22561 gtggcggcca tgatccggac atccccgcgg gtatcgaacc catccatctc cgcgaggagc
    22621 tgcataaggg tccgctgcac ttccgcactt cctgaggtcc cgtcgttggt ccgtgtgctc
    22681 cccaccgcgt cgatctcgtc gatgaagacg atggatgggg acttctccct ggcgagcgag
    22741 aagagctccc gcacgagctg ggccccctcg ccaatgtact tgtgcacgag ctcgctcccc
    22801 gacatccgga tgaacgtagc cttcgcctgg tgtgcgactg cctttgcgat gagggtcttt
    22861 ccggtcccgg gggagccgta gaggaggatc cccttcggcg gctccactcc aaccctcacg
    22921 tagatctctg gtttggtcag cgggtattcc accgcttccc tgacttcctc gatctccttt
    22981 gcgaggcctc ctacctggtc gaaggtgatc tccggcgact catcaagctc catcaccctg
    23041 acccttgagt caaagatgtt tccaacaatc ttcacgatcg agagggcatt gttcaccgcc
    23101 accttggtgc ccggcttgag ctggcggaat atctcgtcgt tgacgtgagt aaggtactcc
    23161 tggttgttcc cctgctggcg gagatagacc tcgccacgat cgagcaggtc cacgaccact
    23221 gcgacaaaca ggggcatccg cttgagctgg ttgttctctc gtttcagctg cgcattctcc
    23281 cgcaggacct cgtcgttttt taccttgagg tccaggacct gcgactcgag gtcctggagc
    23341 ttcagctggt atttgagttc tgcgtcgggt cccgcgttcc gtatgacgct ctcttccatg
    23401 ctaccattat atcttgagtt ccctacattt atgtggtgtg atcatgctca tcaggggtcg
    23461 cggcatctcg aaaggcaatg gtcgcggcag ggtgctgaca agtcccgagc ccatctcctt
    23521 cctctccgga gtggacccgg aaaccgggat gatcgtggag aagggacacc cgctcgaggg
    23581 cgcccttatc accgggacgg tgctcgtatt cccccacggc aagggctcga cggtcggctc
    23641 ttacatcgtc tatgctctca gcaggaacgg gaaagccccg gcagccatga tcaatctcga
    23701 ggccgagccc atcatcgcgg taggagccat cattgcgggg ataccgctcg tggaccgcct
    23761 agaagggggt ctctcccttg tcccggacgg cgcgatcgca agggttgatg gcggggaggg
    23821 gaccgtggag atcctggaag aagaggcacc cggagagaca gcctaatcaa ttcccacttc
    23881 ctacactcac ccatgagact tctgtcctta ctctgcatga tgctgctcat ctcaggggtg
    23941 gcaatctgcg ggtgtaccca gtccccggca ggtagtgccc agtccccgca gggaacgtcc
    24001 cggaccggga tgattcccgg cccgactgtc acgcttcccc ccgagcatgc cgttgagatc
    24061 cagatcaacg agaaagaccc gatatacgcg accatcagcg tcatttttgc ggggggcaag
    24121 ggccaggtca tggtgaccga tatcctggtc cgggtgaccc ggtctgacgg gcaggtgacc
    24181 gagaaacacc tcgaagcggt aaagggcgcg gagctgaagt tcgacgggac gaaggaggac
    24241 gaccgtatag aggccttcgt ctccctcacg aatggcaaca cctacaaggt aatcgaccag
    24301 ctcgtcccct acaggaccag gggttgaccg gggacagggg gagttttcct gtccccctgc
    24361 cagatccatc ctttctccgc tctttataat ggacgtccac tggagattta tccctgcggc
    24421 gaggaactca tatgctgccc tgtacgcggc atgcgagcag gaaggctaca tcctcgaacc
    24481 ggtccggacg cccgcgcctg acgtgacctg ttacagcctc aactcgctca ccgcctcacg
    24541 ctacctcccc gagatacggg atgccccctg catcaccatc gtgggaggtc cccacgcaac
    24601 ggcacgcacc gaagagatgg cccgcattgc cgactacgtc gtggtgggtg aaggcgagtt
    24661 cgtcctccct gccctccttc gctcactgga gtcgggaggg cccgttcccc cgggtgtggc
    24721 gacgaaagag ggcttttccc ccgcgcccca ctgcgttcgc cttgatgcat accctccctt
    24781 ctcgacgatg aagggctaca tcgagatttc ccggggatgc ccgcatgcct gcgcctactg
    24841 tcagacaccc cgtatcttcg ggccgcacat gcggcaccgg agcatcgatg ccgtcaccca
    24901 tgcagcccgg cggttcaggg atgcacggtt cgtgacgccc aacgcccttg catatggttc
    24961 agacggccgg acaccccgcc tcgaaaaggt tgaatccctc ctctcttcgc tccggcatga
    25021 ggaacagcat atctacttcg gtacattccc aagtgaagtg cgcccggaat gggtgacccg
    25081 ggaagcgata gatctcatca ccacctactg tagcaatacc cgcctccatt tcggggcgca
    25141 atccggaagc gacagggttc tttcttcgct ccaccgggga cacaccgtgc aggacgtcat
    25201 atcggccgtg gagctctgcc ttgatgcagg actgacccca gtggtggact tcatcgtggg
    25261 aatcccgggc gaggaggagg atgaccagcg catgactgca cgcctcgtgg aatggatcag
    25321 ccgcaggggg cgcgttcacc tccaccgctt catcccgctg ccggggacac ccatggaaaa
    25381 gacggccccg cagccactcc tgcctgaagt ggaacggctc cttggagcgc tcgcgctgag
    25441 gggaagagtc accggatcct ggggtgaccc tacacgaaga tttttttaaa ggccccctcg
    25501 aatgaatatt gacatgatgg atgtgctttt gctggcagac cttcacggag agttcggaaa
    25561 gctggaatcg ttcctggaac tctccccgga tgccatcttc attgctggcg acatcaccaa
    25621 tatgggccct gcggatgcag tagagtcgct cttttcacgc attgacgtgc cttcgtttgc
    25681 agtgcctggt aactgcgatc cacgggagat catcgacgtc ctggagcatt cggactgcgt
    25741 ctgcctccac ggctcctctc tcaacctggg gaagatctcc attgtaggga tcggcgggtc
    25801 caacccgact cccttcaata ccccgtttga gatgacggac aagcagatcg acgacctcct
    25861 caccgcgctc gtcaggaaga tggagcgggc aacccacaac gtgctcatct cccatgcccc
    25921 gccccagggg ttcctggacg aggctggagg aaaccacgtg ggaagcgcga gcatcaggaa
    25981 gcacatgggt tcctttgacc tggtctgctg cgcccacatc catgagcaga ggggagtcgt
    26041 ggaggagaaa ggggtgaagg tagtcaaccc cggcatggca gcgctcggcc agtgcgccat
    26101 ggtccacttc ggcacggagc caaaggagat caggatcgag ctgctgcagg tctgatcatc
    26161 ccggcctgcg ggaactgcac acaccttttt ttaaaaaaac tacatgattc tttccgaaac
    26221 acacgcatat gcattcatga aaatggcaca gccattttca tagtacccac ctattaccgc
    26281 ctggcggtcg agcataattg atgaaaatca cgaagtgata ttcattcgaa aaaagttgtc
    26341 aaggagcggt gggtccgttc ctactcctct gtcccctggc cttttgcgag gagaatctcc
    26401 aggtatgacg agagaatcgg cttccccgtg acccctatct cctctcccag tgccatgacg
    26461 tattcggcag gccgctcgac attcccggga ttgttcagtt ctatctccac gaactccccg
    26521 agcccctcca cctggtccag gcagacggtt gcatctttga agtggtaata ctccctccac
    26581 ttcctcacct cggcgaccgg ggtaaaccca agcgagatga agatgcgggc catcacgtca
    26641 cctgattcca ccctgcagtt gagctcctcc cttgccttga ggtggaaccc cggctccttt
    26701 ttgcccttgt aggtcaccgt ccagcgctct cccgcctgcc tgatacggag cgcctcgtcc
    26761 gtcaccccga agtccctgtg gggagcattg aggtacaggt cacgctccat caccctcgac
    26821 tcaagctctg cctgctgctc gcagagccgt tcccttaccg cggcgaggtc atccaccctg
    26881 accttcaact cgatctcaag catgctctcc ccttcccatc tcccccatga ggacaataat
    26941 acgtgtggtt tggaacgcgg ggcgaccggc gacagctccc tctccgggtg cagcagaatt
    27001 gtgacccgca aaagaaaaaa aaccaggcga caggagggtg ttcccgggag tgccgccgtc
    27061 aatcgattaa ctatttaaga ccgtccccgc aaatgtataa agaacctcta cccgggaagc
    27121 gccaggattt ttctccccgg gtagccggag attccaggtg aagaaatggc aataaaggtt
    27181 ggagatttta tcagaatcag ctacacgggc agggtggacg ggaaggtctt tgacacgact
    27241 gacgaggata tcgcaaagga cgcgggcatc ttcaaccccg aggcatctta cggcccggtg
    27301 accatccgtg tcgggagcca ccatgtcatc ctcggcctcg acgaggccct cgatgggaag
    27361 gagataggag aggaaggtga agtggacgtc ccgcccgaaa aggcgttcgg accccatgag
    27421 cccacccagg tcgaggcgtt caacaagaac tccttcaagg acaagccaaa gaaggggatg
    27481 agcatcaagg tcccagagaa gggagagggg acggtggtcg atgttattgg caaccgtgtc
    27541 ctcatcgact tcaaccaccc cctcgccgga aaaccgctct cctattcttt ccgggtcgag
    27601 gatattgtcg agggtgcagt ggagcagatg aaagggctca tcaagctcta cgcgggccgg
    27661 gacatggacg tgagcctcaa cgaaggagcc ctcaccctgc accttcctcc aggcatcacc
    27721 tacgaccgcc ggtggctcct ctggaggagc agggtcatac acgagggatt cgagcatatc
    27781 cccacaatcg acgagatcac acttgtcgag accttcgcgc gccccgagaa ggccgaggcc
    27841 gaaactccgg aagaataatt caacgctcac accttttttc caatgaaacg cacatgagac
    27901 tttcgtggta catgcccccc tgatgaaaac cgtggaatgt ggttttcatg ccaaaatgcg
    27961 tgcggcattt tcatgaatgc gaatgcgtgt gtcttggaga gaatcatgta gttttttccc
    28021 cggtcatcga tacgggtgac cggaaatggc gtgaaaagaa agggtccctg aaggtccgat
    28081 caaacggata ccctgcagag cggtgccagt ttccggataa gcctttccac ctgtgcaggg
    28141 gccgtgccga tgtagtgcag gggatcaagg agtgccccga cctcatcagg ggagaggaaa
    28201 cgcatcactt tctcgtctga cgagaggact tttgcaagcg ggaccttctt ttcgagcgcc
    28261 tgcatgctgg ctaggcgcat ggattcgtgc gcctcctggc ggtccatgcc tctccgggtc
    28321 agctcgatca tcactgcctc ggccatgttc accccctgga ggaccgagag gttctgagca
    28381 atccgttctg gcttgatggt gagcccctcg atcaccctga tcatcagggc aaggatgtgg
    28441 tcggcgagaa tagtcgcctc ggggaagagg acccgctcgc acgaggagtt ggtgagatcg
    28501 cgctcgtccc agagagtgtt gttctgcagg gcaggctcca ctgccgaccg gaccacccgc
    28561 gagaggccgc agacctgctc gctcttgatg gggtttctct tgtgcggcat ggtgcttgac
    28621 ccgacctgtc gggccccgaa cgcctcctcc acctcgccga tctcagtcct ctgcagtgtc
    28681 cggatcccga tgccgatctt gtcgagggtg gtggcgacat ttgccaggaa catgaaatac
    28741 tccgcgtacc tgtccctcga gacgacctgg ttcgagacgt ctaccgccgt cagcccaaga
    28801 tactccatca ttgtcgcctg gatctccatt cctctctccc cgagtgccgc ctgggttccc
    28861 actgcgccgg tcagctgccc gacctccacc cgcggggcca tctggccaag ccgctctatg
    28921 tgccgggcga cttcgctcgc ccagatggcg aaccgaagac cgtaggtggt gggcaccccg
    28981 atctggccat gcgtcctccc cgcacagaca agcatcttcg tctcatcggc ccgcttcagg
    29041 agggcggcaa gaagcctgcg gaggttccct tcaaggatgt cgagagccgc cctgatctga
    29101 agaccggttg cagtatccag gatatcgttc gacgtggccc cgtagtggac atagccccct
    29161 ccctcgccgc actgctctgc aatggccttc acgatcgcca tcatgtcgtg gctgatctcc
    29221 tcctcgatct ccttcgccct tgcaagcgtt gccttcccgg cgcacgaagc gatagcctcg
    29281 gcggccgcgg cggggatgaa caggtgtgct gccgatgctc ttgcaagggc cacctctgcc
    29341 tgcacgatgc atgagaaccg gtactcctcc ccccagactg cccgcatctc tgcggtcccg
    29401 taccggaact cgatgggatg gattgccatg ggagaagggt tggtgtttcc cgataataaa
    29461 aaagagtcag ggctccaatg gtatggtacg atggacagcc tcgacgactg gttcccctac
    29521 gcggattatc gcccatgcca gcgggagatg cttgaattcg gcctctcgtg cgcacaggaa
    29581 gggacgatcg ggctgatcga tgccccaacc gggagcggaa agtcgagcct cctcgcagcg
    29641 ctcctcgcgg ggagggagaa gcgccgcgtc ctggtcactg tacgaaccgt gagccagctg
    29701 aacacgttca tccgggagct ctcgctcatc agggagaaga agccggggct taaagtggcc
    29761 tacctcattg gaaagagagg gatgtgcccc ctctcgggag agggggatgt gtatcgccgg
    29821 tgcgagggag taaaggggtt cagtaccacc ctgatgcgcc agcgagccga gagagggtcg
    29881 ctcgtgcctt ccagggaccc gtacatccag cagcagatac ggcggatgga cccccaaagg
    29941 cccctgctct gcccccactt catcgggagc cgcgtctttc tccagtcgga gggcggcctg
    30001 cgcatggtcc cctctcctgc actccagaca aaagccgaga gagtcaccag ccagacagta
    30061 tggccccacc agctgccgga agtggcctca ggtgtctgcc cctacgagat gatgatactc
    30121 gcagcccaga aggctgacgt gatcctgctg aattaccggc acctctttga cgaggatatc
    30181 agggaagccc tgtacgcaag cctcgccctc gacccggccg atgccatact gctcgtcgac
    30241 gaagcgcata actgcggtga tgcggtcacg gccgcccaga gcgtggatct ggaccaggac
    30301 acgctcaccc ttgccggtac cgaactctcc gggctacgcc gcgcgaaggc gggagtcgag
    30361 gccctccagc aggtgatccc gcgcatctcg gggttcatgg aaggactgca gaactccttt
    30421 gagaccgagg actggttcga cccggcaatc tttgacagga tggtagtgaa gggatcgctt
    30481 taccattcaa tggcagggat cgtggacgac ctggaggaga tatcgggaaa gttgcgggag
    30541 aagaatatca aggccgggga gttccgggag actgcgctcg agcgcctcac ccggttcctt
    30601 caaaagattg ccgcatcctc gaaagatcct gcctcgctca ccctgttcag aaaggaggac
    30661 ggtctcgtct cccttgaggt tcggagcata gacccggggc ctccgctcca ggagatcgca
    30721 cgttcccacc acgcatgcct gctcatctcc gggacgctct cccctgtcga ggcatacgcc
    30781 aggttttact tcggcgacct ccctgcaagg actctctccc ttccgaatgc cttcccgaaa
    30841 gagaatcgcc ttgtcgtgtg tgccagcgac atcaccaccg ccttctccat gcggcagaac
    30901 gagaagaacc tccagctgat acgcgcatac atcgaggcat tctcccgcct ggacggaaac
    30961 ctcgccgtct acttcccatc gtaccagatc ctggagggat tctcaaagga tttgacaacg
    31021 gcaggcagga aaaagaaaaa aatcttcgtg gagcccaggg acgcaaaaga tgcagaagaa
    31081 tccctacgcg agttcctctc tctccctggt tccgggagat ccgggcttct cctcgctgtc
    31141 tgcgggggga agtggagcga aggcctcgat taccgcgggg atctcctgca gggcgcgatg
    31201 gtgatcgggc ttccccttgc tcccttcaac caggtccgaa ggatggtgat cgggtatttc
    31261 cgccggaggt tcggggatga gggggagttc ctgtgttaca ccctcccggc catcaaccgc
    31321 ggccagcagg cactcgggag ggtgctccgg actcccgacg accggggagt tctcgtcctg
    31381 ggggagaaac ggttccttga ggggaaggtc tccaggggtc tcccggaatg gatgagaaac
    31441 gagatgcgtt cgtgcaccct cccggagttc gaggatctgg tcgggaggtg gagatgagag
    31501 aggggtcatg ccggttcggg ctctggcacg tgcacgtcca ctggtcaaag gacaccgtga
    31561 ggctggtccg gttctcgacc cggcctctcg ggggagacgt gccgctcccg atcaggagat
    31621 actgcgccgg cttcccggaa ggactgcatg aactggcaag tattgccctt tctcccgaat
    31681 acccgttctc ggccatctac cggcgggtaa gggagatacc gtacgggaac acggagacgt
    31741 atggcgagat cgcgcgcgac ctcaacacct ccccccgcct cgtgggcctg gcgatgaagc
    31801 gaaaccccac cccactcgtc attccctgcc acagggtcgt ctcgaaaact gggacagggg
    31861 gattttctcc cgaccccgcc atcaaggagg cgctccttgc catggagcgg cgaaacacaa
    31921 ggtgtttatg aggataaggc ccagtcttgg aggaaaggcg tcccatgact tcgcgtgtcc
    31981 cgatggtctt cgaacgcgtc atttcaaccg cggcatcttt catgagccct ggccagcaga
    32041 ccgcgacccg tgggatgtct ccgcatcgat acgacctggt gctgcctgga gggccctgat
    32101 ggaccgctct gcggtgatcc ttgtgggcgg cgaggctaag cgcgccaacg gtcttgagaa
    32161 gtacttcttc acgttcgggg gaaagacgtt catcgagagg ctgaccgaca cccttgcaga
    32221 ggtggttgat gagatcatcc ttgtcgcaaa ggaccctgcc cagtgcagcc gtttttccca
    32281 cctggacaat gtccggtgcg tgaccgacat caggcggggg tacggcccca ttggggggct
    32341 tcacgccggg gcacttgccg cgaagggaga tgccctcttt gtctgtgcgt gcgacatgcc
    32401 ctgcgtctcc acgcaggtgg ttgagagtct cttctcactc ctcgaagggt acgacgcggt
    32461 catcccgcaa tgggcgacgg acatgatcga gccgctacac gcggtatacc ggcgcagtgc
    32521 cctgctcagc gcactcgaga gccacgagtc cctctccctc cgtagaatgg tgaaagggat
    32581 tcgcacccgc tatgtggatg tggcaggcat ccgggaactc gacccggagc tcaggacatt
    32641 catcaacatc aacaagatag aggacctggg aaagctctca tgaggggcag gcaagggttc
    32701 ccgcgggggt gacgtctgca cctgccgggg cagggacccg catctggacg gtggccacga
    32761 tatcgttccc cgagaactcg accagatact ctcccgatgt ccgtacctgg aggaccttct
    32821 ttgtatccac gctataggtc ttcccaaacc cttccttctc gacaatgtct ccggacgcct
    32881 tgtccctgac cctgatctcg aagtacgacg ccgggcttac atacgtctgg gtggtataga
    32941 catcctccct cgaggtatac ctgctctcgt accagatatt cctcgtcacc atcttcgggg
    33001 cgaggcatac ttcgatgatc agcgggggat tctccacggt cgtggaatag gcggagacgc
    33061 tgtacttgaa atcgatggtg ttcaggtaga tgaccttgta gaccgtgggt tcgaatgtga
    33121 aattcggcgt gctgctgtag gtggggcggg tggttgctgg cgttccggtg ggaaaaggcg
    33181 tcgccggcgt cacgtattgc gtgtccatta tctcctgggt cgggctcggg gtcggaacgg
    33241 tcgtcgccat tcctccattc tgggtcatgg gtccgggagg ggtctctttg ggcggggtga
    33301 tgcacccgga gaagaggagg cagacggcga ggacaaccgc gagtgcagga gagagcccca
    33361 gaagcttcat tcaatctggg ttgaatgccc gggtatttat tagtttatcc tgcgaggcag
    33421 cgcccgtgta tgccagggac ctttccgttc tttcccttcc ggctgccata cccttatata
    33481 aatcccggtg gcataggtca atggttatcc ccgggttggc tgcaatggag aagaagtccc
    33541 agttctggaa gaagaccgac aagtcgctca ataccgcaga cgggtatgtc ctgaaagccg
    33601 atctcctggc ccatgaccag aagttcgagg aggccgtgga atactacgac aaggccctcg
    33661 agatcgtgcc aaaaaacgcc gatgtatggg cattcaaggg aatcaccctc caggggggcc
    33721 tcgggcggga cgaggaggca cgggcgtgct gggagaaggc aaaggcgctc gaccacgaca
    33781 tcgccatggc cgttgacatg acaaaagagg agccggcaag aggcgaggac cttgagtcga
    33841 tccgctggtc cgacctccat gacagctgca gggagaaact gaagcggatg atgctgaaga
    33901 acgtcgatgc agggacacgc aagtgatgcg tctacagacc tggtaaccac cgagtcgcca
    33961 aattttcccg ggaccggaaa ttcgaagccc caatcaggat tattctgcga atatccgtat
    34021 catttcttat gggctatttc caactctacc agcagcgggg cgatcgtgcc ggtatcgacg
    34081 cacctctttg caaggcgggt gagccttcgg acaaacatga tatgtgcgac ctggaaactg
    34141 accaggaggc cgaaacagag cgtgacgatg ctgatgacga ggtatgcggt gaaatctacc
    34201 attgccctaa cctcccttcg tccccgctca tgactccctc tccatttccg tgatcagggg
    34261 ggcgatggag tccgcgcgct cgcattcctt cgccaccgaa agaaaccgcc tggaataata
    34321 ggtggtcatc aacaggtatc ctgccatgag gccgatgcag aacatcagca cccctatcac
    34381 caggtacacg ctcacgtcat ccatcgcttt actcattgac tgatacagaa ggacgatata
    34441 tatattttcc tcattctgcc cagactgcgg gaatttatag atgagagatc cctggccggc
    34501 atgccctacc ggctgatccc ctcgataaag acctgtgccg ggaggtggag ccttgcatgg
    34561 aatgcagatc ccgggtagat ctcctgcctg atctcaaggc tgcagcggtc cagttcgcct
    34621 ttctggtccc gatcaaacct ctgttccatg gcctcgtaga accgaagaag agattgctcc
    34681 ggatgcgggt cttcgcctgt caatggcagg catgcaagga gacgccggcc ctcttcaacg
    34741 ggaaggtccc ggggatgccc gtcaacgtga aggagggccc tgtggcaccg cggcaccccc
    34801 tccgtgaagg agaactcaag ggagatgtcg gcccggtgga ggccctgcaa cgccaggctg
    34861 acgaaattga ccaggaaatc gtggaaggca gaaaagttct gggcatgttc ttccttcagc
    34921 gggccaatcg cctcgtccct tgtcttctca gggatagcgg tctcttcctg cagcgccctg
    34981 atgaggcgct c
//
LOCUS       DAOQYR010000016        32525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_1602_length_32525_cov_8.637512, whole genome shotgun sequence.
ACCESSION   DAOQYR010000016 DAOQYR010000000
VERSION     DAOQYR010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 32525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32525
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1602_length_32525_cov_8.637512"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PKM87_03545"
     CDS             <1..197
                     /locus_tag="PKM87_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HNQ29173.1"
                     /translation="PSNKSKGYGKPFNEIFKEAGYDFYKIDTSLFSPAEVVINEISEG
                     VVYHCGAVNPEVTLKSFGLI"
     gene            321..980
                     /locus_tag="PKM87_03550"
     CDS             321..980
                     /locus_tag="PKM87_03550"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ29174.1"
                     /translation="MSGMDASREAWDTDYAMRGRRWARGHCRMPSFHPHERVLDAGCG
                     DGKGLASLACNGDPSNMAGARCPEVVAFDFSRSAIGICAHRFSQNEKVHLLTADAARM
                     PFRAASFDAVLLFHLIGHASRRPRSEICEEVQRVVRRGGYVYLRVFSRADFRAGRGEE
                     IEEMTFLRGDGTFTHYFLPEEIAALFPQLTLISIEHVPWILRVKGDRFQREEIHAVLR
                     A"
     gene            1102..1175
                     /locus_tag="PKM87_03555"
     tRNA            1102..1175
                     /locus_tag="PKM87_03555"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1136..1138,aa:Val,seq:tac)
     assembly_gap    1775..1874
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1875..2113
                     /locus_tag="PKM87_03560"
     CDS             <1875..2113
                     /locus_tag="PKM87_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29175.1"
                     /translation="NGFFGAPFSGRRLPEKALYNQGGINIGSTPGFPKTGRFSVHQNE
                     ISHCDKKGIGSHRAARPGAIQVTPYKGLFLIMLL"
     gene            complement(2187..2723)
                     /locus_tag="PKM87_03565"
     CDS             complement(2187..2723)
                     /locus_tag="PKM87_03565"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNQ29176.1"
                     /translation="MALAMSGMVLLLVIIAGCSTTSGVKAGDTIRVFYTVSFPGTEPF
                     EGNVNGTPLEFTVGDGTVIQGFENAVIGMNPGETKTVTISPEEGYGLRNETLVKEVDI
                     ETALAFVQNLDQMGTSRILFFPGMEPVFEYTFPGNVVVHYTFYNITGESTTIDQNHPL
                     AGKDLVFTITLEEIVKKQ"
     gene            2954..3847
                     /gene="nifB"
                     /locus_tag="PKM87_03570"
     CDS             2954..3847
                     /gene="nifB"
                     /locus_tag="PKM87_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
                     /protein_id="HNQ29177.1"
                     /translation="MAENEYKVAEVAGKKVQWDPEQLRKIREHPCFSEKACHAFGRCH
                     VPVAPKCNIQCNYCIRDFDCVNESRPGVTSKVLNPQEAVTLVRDAIQKFPYIKVIGIA
                     GPGEPLANEETFETLRLLKEEFPNVIKCLSTNGLLLPDKIDLLHKYDVGNLTVTLNAI
                     DPEIGAKIYQFIDYKGKRYEGVEGAKVLLKNQLEGIRMAIERKMIVKINTVYIPGINE
                     DHIPEIAKKVGEMGVYNFNVIPLIAQYRFSHITPPTPEMKVKMQDECAKYVRQMRHCQ
                     RCRADAVGRLGQDVQSCLYHE"
     gene            3948..4139
                     /locus_tag="PKM87_03575"
     CDS             3948..4139
                     /locus_tag="PKM87_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29178.1"
                     /translation="MPVSEEIHPVAIANEFRQCRTCGYDRGFHTSLHRIAAGHPHFRV
                     VLICPECGTRYDARWVMEI"
     gene            complement(4163..4552)
                     /locus_tag="PKM87_03580"
     CDS             complement(4163..4552)
                     /locus_tag="PKM87_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29179.1"
                     /translation="MDEALPAGEKPSPPGVLIEYSRTGGIAGFSDYMVVFSDGWVVYN
                     STHHGTGGFSLENRDVDLLRGLIRDANIPSLKDEYPAPVQGADYITYVIVVGNRTITM
                     ETTSVPAQMVPVITTMDDLLGTHGSAP"
     gene            complement(4994..5320)
                     /locus_tag="PKM87_03585"
     CDS             complement(4994..5320)
                     /locus_tag="PKM87_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29180.1"
                     /translation="MELNEVLRAFLEGGEDWERKNTSLKGVSIIKLPGTKSRAPSLAI
                     EVNPVNEKGAPMKKKGIMIMSASELSAFREIFSNPKVDGLIRSIEEITPGKKSQKSGA
                     GDILEI"
     gene            5546..6427
                     /locus_tag="PKM87_03590"
     CDS             5546..6427
                     /locus_tag="PKM87_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HNQ29181.1"
                     /translation="MPSISLRPDQPFDLDLTLSCGQVFRWERDARGVWSGVVGDSLIR
                     ICQQGQSLTFEGADRKFIQTYFQLEEDLPAILGSIDRDPLVHSAICRCRGLRIVRQPG
                     WECLASYIIATFANIPGIKRRISHLCRCFGNPFFGSGGECYTFPSPETIAFREPCRIA
                     ECRLGYRAPYLKETARLLAGDPGWEERLRELPYDAARRDLLRLKGVGKKVADCVLLFA
                     FGKYEAFPVDVWIQRILATRYPGGESLRTYDQCSRFGRSHFGEYAGYAQEYLFCDRAI
                     LAGEGRGDQVPVSQPER"
     gene            complement(6397..7623)
                     /locus_tag="PKM87_03595"
     CDS             complement(6397..7623)
                     /locus_tag="PKM87_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNQ29182.1"
                     /translation="METGEKKMAWARQFMPVLSSIREEFIREMPFRGIRVGMALHVEA
                     KTACLVETLAAGGADVYITGCNPLSTQDDVAAALLRVKGVHCYAKRGATTGEYYAAID
                     RVLDARPQVTIDDGMDLIHRLHTARTDLLPDVIGGCEETTTGVHRLRAMAGDGKLRFP
                     VIAVNDTPMKRFFDNVHGTGESALSAIMLTTNMLIAGKAVVVAGYGFCGRGLARKLHG
                     LGARVIVTEVDPRRALEAYMDGFLVMPMDAAAERGEIFITTTGNTSVITARHFRVMRP
                     GAVLANAGHFNVEIDLQWLEANAGSVTRRDGIDTYRVDGKEIHVLAEGRLVNLAIPKG
                     MGHPIEVMDLSFALQALCARHIVSCGRSMSPGVYEVPSDIDDAVARKKLASLGLSIDE
                     LTPEQRDYLSGWETGT"
     gene            7759..8565
                     /gene="hisF"
                     /locus_tag="PKM87_03600"
     CDS             7759..8565
                     /gene="hisF"
                     /locus_tag="PKM87_03600"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNQ29183.1"
                     /translation="MTLTRRIIPCLDLKGGRVVKGTHFLDLRDAGDPVELAQRYNEQG
                     ADEVVFLDITASKEQRGTIIEVIERAADQLFLPLTVGGGIRSVEDIQQILRAGADKVS
                     INTSAVHDPSLLSRGAEKFGTQCIVLAEDVKRNFSDNPAATPVTLSDGRTCWYEVVIY
                     GGSKPTGIDAVAWAREAEERGAGEILLTSMETDGTKNGFDIPITAAISEAVGVPVIAS
                     GGVGTLEHFYNGFVRGKADACLAASVFHYGEMTVRQVKEYLARRGIPVRL"
     gene            complement(8522..10162)
                     /gene="pheT"
                     /locus_tag="PKM87_03605"
     CDS             complement(8522..10162)
                     /gene="pheT"
                     /locus_tag="PKM87_03605"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNQ29184.1"
                     /translation="MAVITLYYRYLERLVGSDRETILARLPMIGSDIERVEEDHVDVE
                     FFPSRPDLFSVEGVARAMRGFLGLETGLPQYHVTPSGISFSVDPGLASIRPYLGTAVI
                     RGIQLDEEAILSLMGLQEALHWALGRGRSKVAIGIHDLDRVTPPFRYIAEERTRAFVP
                     LDYEREMTMEEILDEHPKGRAYADIVRGFPKFPLIVDACNQVLSFPPIINGELTRVTG
                     ESRNLLLDTTGTDERAVMTAVNIICTALADAGGRIESVDVAGNHIPTLAPSTRLVSAR
                     ECSALLGADLTPAGLSLLLGKMRFSAEPAGDDKVRVMVPCYRADIMHDWDIFEDAAIA
                     YGYENFRAELPPTFTIGRAHPVMIVASAVRSLGSGLGYQEVIPFTLTSEKVMYGKMQR
                     PVAPETLSVLHPISEENTIVRTGLLPLLIETLQFNRHRELPQRLFAVGDVVRDLLTFQ
                     KAAFVSLHPAADFSEAYAVADAMCRELSIPYTSEESEDPAFIGGRRGSIVAAGRPIGV
                     FGEIHPAVLNAFELEHPVAAVEIDLTAVPGYPAGLGTP"
     gene            complement(10164..11612)
                     /locus_tag="PKM87_03610"
     CDS             complement(10164..11612)
                     /locus_tag="PKM87_03610"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNQ29185.1"
                     /translation="MDLSLNEKRLLRVLEPLKRADPATLAARMETTAESVIQFAFLLE
                     NRGLANVMKKVDTTYHLTEEGARYARDGLPERQLLESFGERVPMSELTTHPLSRVGIG
                     QMRKKGWVKVCDGVVEKTGRDAPGEDEVALSDLSRGGDGVAILQKRGLLTEHQETRYL
                     VSLTPEGEALAAKGLDLEEEVGTLSREQIKSGAWKDLRIRRYSLETLPRKVFPGKVHP
                     YQRILDEMRQILLEMGFSEIYGGLAQSSFWNFDALFQPQDHPAREMQDTFFLKETVSL
                     PAGFERVRDMHLHGGTTSSTGWGGQWNREKAEQCVLRTHTTSLSIQHLVAHPEPPVKV
                     FCIGRVYRRESIDATHLAEFEQLEGIVMDRDVTFRHLLGFLKEFYGRMGFEDVRFRPG
                     YFPYTEPSVEPEVYVDGLGWVEMGGAGIFREEVRAPWGVTHPVLAWGLGVSRVAMLRL
                     GLKDLRQLYRTDIDFVRETPLWHQPGKTRGGA"
     gene            complement(11614..12870)
                     /locus_tag="PKM87_03615"
     CDS             complement(11614..12870)
                     /locus_tag="PKM87_03615"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNQ29186.1"
                     /translation="MQAPLNPWSSQQSDDIEKLFSMFGIEPISSIDGILPHVPSFISR
                     GVVVGHRDYRQIAEAIRDNRRFHVLTGFMPSGHPHLGHLMVMKEVVWHVQQGGTGYIT
                     IADREAHAVRDIPWQKCDEYGKEYLQCLHALGYQGHTYFQSRNKRLMDLAFEAATKVN
                     FSELQAIYGFSDETALAHAESVITQVADILYPQVDSPPAPTIVPVGIDQDPHIRLTRG
                     VAHKLRMFTVEERDGYISVRSKNAPPAALGEVHRRFAGSKKYEGHVDIPGGRCTDVSV
                     EVRNIEIAHGGYGFFTPSSTYHRFIPGLQGGKMSSSVPESTITFTEPDEVVRKKVMAA
                     LTGGRPTLAEQKEQGGEPDRCPLYLLNLFHMVDDDAELAEIRRRCLAGELMCGQCKKE
                     TAERVLAFVRDFRERMEAVAHLVKVE"
     gene            complement(12882..13889)
                     /gene="endA"
                     /locus_tag="PKM87_03620"
     CDS             complement(12882..13889)
                     /gene="endA"
                     /locus_tag="PKM87_03620"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HNQ29187.1"
                     /translation="MKATFDGTWVRAGHEGRALYDQSGYGRPEKEGIRLAPEEALYLV
                     HRGRLEVAGYSFDRLLAVCAERPEFMRSYLVYRDIRERGYVVQTGPHDFRVFRRGERP
                     GTGQSQYLVRVISERDLIDFSGLLGEAAASLNLRKQHVLAVVDDENELTYYEVKMPTL
                     PQVEKEEEEWNTRGELVGKYAIVHVPPSGSAVPGSYGMQLDPGRLVLAPLEILNLMRS
                     GRLTLQRNGEPIDPERYYGMAHESDIEFPEKVAVYEDMRNRGFVPRTGYKFGHHFRVY
                     SGKKVHSEMLVQALPAGASLPMSAISRSVRLAHSVKKKMLFGCEHSQGIQYVEFARIK
                     L"
     gene            14224..14847
                     /locus_tag="PKM87_03625"
     CDS             14224..14847
                     /locus_tag="PKM87_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S single cluster domain-containing protein"
                     /protein_id="HNQ29188.1"
                     /translation="MRWRFSTRRGEGDMINCAGFLERSAVNGPGRRAVVWVQGCPLRC
                     PGCFNRDLWSFRKANFVHEEMLAERILSIPGIEGVSFSGGEPFCQAAPLATLGKIIRD
                     AGRSVVTFSGYPIENLLRSRRQDWRALLQETDLLVAGPYLQELRMESSLCASSNQVAA
                     FLSGRITPYQEEADKGESVEFTICRDGEITTTGFPKRAPLPFERGCT"
     gene            14851..15246
                     /locus_tag="PKM87_03630"
     CDS             14851..15246
                     /locus_tag="PKM87_03630"
                     /inference="COORDINATES: protein motif:HMM:NF018565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1257 domain-containing protein"
                     /protein_id="HNQ29189.1"
                     /translation="MSHFSKVKTAFKDQSLLAQCLEEMGYQVETGGTIRGYHGVQQVD
                     LSVAGRDGYGVGFVRNGDGCYDLLADSWGVKGADKKILDSLRRNYPRIQQEYARRTVR
                     EQAEREGYTMVEEVEQEDGSVRIVVRRWA"
     gene            15243..16811
                     /locus_tag="PKM87_03635"
     CDS             15243..16811
                     /locus_tag="PKM87_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ29190.1"
                     /translation="MRTDASEQDIPSQVDLCLRARITLLILHTAEEERAIRMLQDVCG
                     RFSPKRPFFTWDHSDGFAGPAGTPCRGSSARDPLVALEEMEKTDEASVIVLKDFHEFW
                     GNPGVKRRLRNLAQKFRFSRRTMVVVTPSLKVPEEIRDDAVVLHVPPPSARDLEHELD
                     RLLRGSGAGDSLTPLGREKLIQAALGMSLNQACRSFSKAIVRRGRLDDRDIDSVVSEK
                     KDILSQSEALEFYSATETAENVGGLSVLKEWLRLRERAFSREAREYGLPSPKGIALIG
                     IPGTGKSLTAKMIAGMWRLPLLRLDVGALFGSFVGESEERTRRALALAETISPCILWI
                     DEMEKAFSFGNGDSGTSQRVFATILTWMQDKTSPCFVVATANDISALPPELLRKGRFD
                     EVFFLDLPSLGERREIFSVHLRRRGLLPDDFELDRLAAASEGYVGAEIEQTVIDAMYH
                     AFNQDMRRVTTGDILTALGAQVPLSISQKEVIGGLRAWLTEGRALSASPPATPAGIPG
                     GSICLEPVVSPANR"
     gene            16818..19664
                     /locus_tag="PKM87_03640"
     CDS             16818..19664
                     /locus_tag="PKM87_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNQ29191.1"
                     /translation="MPAMDRMITRVSCGTPLIGRLIRSSAITGLAVAARCGDNGAVRA
                     LSRAFVDSVHPDVRDAARECLSSLESPDALEAFCDCVLGEGDPRLISIARKCGYLPRS
                     DERKALFLFLTGEIAAWRELDLLEGHPLLEKSLLSAPPDIRSRALAQAREQGIARLFH
                     ALADRPDAPGWSTGDWSAHFDFCRKERRWDELYALLFLAPLPYAVDAVQTLRVSGYKP
                     PDTDLALWKDLLALVPDRWRYPELPEAPSGMLSGPGGRVEQAAFSPDGTHLATLGCDG
                     TLCQWSLPGGVLLSSCRPACGGITTLAYAPGGLALVLGGNDGRVRVLGTGNSTEIREW
                     QAHGSAVAAIAFPPEGDTLLSAGFDGTVCTWTWPGGEVADRRHLHDCAVAALACHGDL
                     VASGGRDGTVMLWKRGDMEYAEIQGGGDAIRGLTFSCEGAALAGIDERGTFRTWNCED
                     GALRMLTPSHAKRFIAWCHTPSGDFGAMATDEHTAVLFSPSEAREVLRFDVPGQGITC
                     LALHPGGAFLLAGCRDGYLHTWSIPEGKRLHFMKAHQGQVQLLAVNSSGTGAISAGWD
                     GSVRLRALPGGELQTVMQGPGAAIRCLAATPCGEVLSCGSEGGILMVWETRSQELVHW
                     HNLFCGRIECLALDPSGTLAACGDAQGRVSLWDIETGSLRATLEGHEGGVHALAVSLE
                     GDLLASAGWDGVVRVWSLPEATHVASLEGHGSAVTSLSFSPAGRFLVSGSHDRSAIVW
                     DLESCEMHARISGHSHVVSCLGISGDGRILATGSWDRAIRLWSLPSGAPMGTPLIHAG
                     RVRSLAVHPDGNLLVSAVDGGTISIWALPGGELIRNRVVRADARNGICIIPGMHWVAS
                     AGLDGSLNLSGLPWAKPMSRTTPADIGYVQTCISRASSHQDIRQWKFLERLLAGRFRQ
                     CIAYGGAGMPAGPHDIEIVEEPVRSEAVACGVRM"
     gene            19664..21256
                     /locus_tag="PKM87_03645"
     CDS             19664..21256
                     /locus_tag="PKM87_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNQ29192.1"
                     /translation="MPLRVSIDFGTSNTVIACWDEGQGSARVREIPGWSRPCNAEPRS
                     TPVIPSLIHYGGEEARWIGQQVHDRGLLDSPSTVRWMKHYISTRSPVRVPLPGGSRSY
                     REVGADFLQAILSSILPAQGCEEIEAVFPVPVESFEHYQDWIISLAGSAGIGRVRVVD
                     EATAAAVASGFTLIPGEVFMLFDMGGGTLDVSVVRLEDKGDSPGRRCRVLGKAGDDIG
                     GMRMDQWIFGEVWERCMSTCDERSRKALSRPLLSACERAKEALSLGEETEISACTGTH
                     DRCELSIPYTRTEFERLMEEKGVFPRLNAVIQRALQGAYSRGFAEDDLAGVVMVGGCS
                     QIPAIKKALEYRFGRQNVRHAHPLETVARGAAACAAEIDLDDHVQHDYALRFWNPRSA
                     EHEYRILVRQGDPYPSIRPVARLLVRATYDGQSQLGVPIFEMGKARGNARSSQRELVS
                     EPGGGVRLMDIPDDEQAGTHAFWMNENAPFFITANPPAARDESRFELSFSLDGNKRLL
                     VTARDNRTGQVVVKDQPVVRLV"
     gene            21271..21492
                     /locus_tag="PKM87_03650"
     CDS             21271..21492
                     /locus_tag="PKM87_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2997 domain-containing protein"
                     /protein_id="HNQ29193.1"
                     /translation="MEMQELEIVIGRDGQVAVSVKGARGPGCLALTRALEERLGDVVG
                     RTHKAEFYSSSEGAMVQERPLENVGERRA"
     gene            complement(21606..22460)
                     /locus_tag="PKM87_03655"
     CDS             complement(21606..22460)
                     /locus_tag="PKM87_03655"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HNQ29194.1"
                     /translation="MVRVGVHVSIAGSISLAVERAADIGCDTFQIFSRNPRGWGYRDL
                     SPGDVEQFKESLAGSGISPVFDHMPYLPNLATPREDMYEKSCATLAAELLRCGTLGIP
                     FLVTHLGHHLGDGIEAGRARVIHAVNKALEAAPNRVMLLLENTAGEKNGVGSTFEDIR
                     AVMDGISRQERVGVCFDTCHAFGAGYELRNSKGLAETLGRLEEVIGLAHVRLIHLNDS
                     KGTLGSGLDRHEHIGLGSIGEEAFSLMLHDDFFRTRPLICETPVDERRDDRGNIAKVR
                     ELAGSRRG"
     gene            complement(22574..22945)
                     /locus_tag="PKM87_03660"
     CDS             complement(22574..22945)
                     /locus_tag="PKM87_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ29195.1"
                     /translation="MGKVLLVDDSELTRYLLGQILISGGHEVVGEASDGVEALQKVRE
                     TNPDVVILDVIMPKMRGPQTLEEIRKISPSTRVIICSGDHQEYTIRDVVKKGAAGFII
                     KPFKKEAVLQEINSVLAGVGK"
     gene            complement(23004..23975)
                     /locus_tag="PKM87_03665"
     CDS             complement(23004..23975)
                     /locus_tag="PKM87_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HNQ29196.1"
                     /translation="MRVAVVEGDGIGHEVIPVAKRALEVLRPDMEFFPVEVGYGRWER
                     TGQAITEDEIDALASADTILFGAVTTPPSPDYRSVIVRIRKELDLYANLRPVWGEGFD
                     ILVVRENTEGLYSGVEKIEQDSACTVRCITRKGSERIARFACGFARSRTGLTIGHKAN
                     VLKSDVFFRGICIAEANAAGVPWKECFIDALCLDILQHPERYDVIVTTNMFGDILSDV
                     AAYLVGGLGLLPSGNLGERHALFEPVHGSAPDIAGKNLANPIAALRSASMLLMHRAND
                     PESARLIETAINRVRARDVRTRDMGGMYGTVEFGNEIIRELESLIRR"
     gene            complement(23972..24775)
                     /locus_tag="PKM87_03670"
     CDS             complement(23972..24775)
                     /locus_tag="PKM87_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29197.1"
                     /translation="MIFPRLCKEVGYASSRPCGDKVYFLSRFLVRETEDGPELLEVHL
                     DEKGPGLMRAIRNECVLAAPGEVTVYPEKVQIHDRALLVRLAMESGTRCTIFTGLDEH
                     STFVLDPDIGAFQTVHVYDVVPPRPSLSSAIRELEATGLFGDLDVVFEHSLQDISLQQ
                     ADVYPCRAAGFTHTLDADPLEGGERVAGCMTGAQLIRECYGDDFDIVEICPLKMVKDE
                     PFIARCCRKEREGVGVYNGKIGAVVHWGANPAQIYHAVCRLLERWRERA"
     gene            complement(24772..25242)
                     /locus_tag="PKM87_03675"
     CDS             complement(24772..25242)
                     /locus_tag="PKM87_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HNQ29198.1"
                     /translation="MIIEGHALCLGPDIDTDVIIAGRYLRTRDRSEWARHAFEDLDPT
                     LSSRLEGTVILAGKNFGCGSSREQAAIALKEAGVRGVIAPSFARIFYRNAINVGLPVI
                     ECECTCTDGEMVRADPAAGKVAIGDRSYLTRPLSPRMREILSAGGLVSYWRGRR"
     gene            complement(25239..26453)
                     /locus_tag="PKM87_03680"
     CDS             complement(25239..26453)
                     /locus_tag="PKM87_03680"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HNQ29199.1"
                     /translation="MSTLSEQILGAPAGDYVDCPVDRSYVHDGTGVLTLEAWRAMQAG
                     VLARPDRCYVLFDHIVPANNSVTAGLQQDLRMFAREHGLNFTDVGEGICHQLMGEGIA
                     LPGEIIVGADSHTCTLGAFGAFATGVGATDMAAIWAEGGTWFRVPETIAIRLHGALAL
                     PAEAKDLALTYVARLTMDGATYRALEFVGDGAATLSIDSRMTLSNLAVETGAKAGLFY
                     ADGATCRYLEGYGHHARPQQPGECCYERELHIDMSAVVPVVAVPPRVDTIRPVEEMEG
                     LPLDQVFVGTCTNGRYEDLQRFSAMVKGKRVKVRTLIAPASRNVLARATGSGVLSDLV
                     RSGCTILPPGCGPCLGAHQGVLGEGEVCLSTANRNFRNRMGVGGEIYLGSVSTAAASA
                     LAGALASPGAVR"
     gene            complement(26450..27580)
                     /locus_tag="PKM87_03685"
     CDS             complement(26450..27580)
                     /locus_tag="PKM87_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase family protein"
                     /protein_id="HNQ29200.1"
                     /translation="MRSWHIEICDVTLRDGEQTPGVSFTCEEKTRIAEVLDQIGVEVI
                     EAGFPATSPYEKRCVSAVASLGLDARICCLARARRADVDAAIEAGVDMVSIFMPTSEL
                     HIRHKFHAPRENVLDEALRMVDYTHDHGLQVRFAAEDASRTDLPFLMEVYRRGEEHGA
                     DLLSFADTVGALTPLQMYRTMRKIVRAVGKPLCAHCHNDMGMATANTLAAAEAGAFQL
                     HTTVNGIGERAGNAALEEVLVGLHMKGGVRRYDLSHLVPLSEMVVHYSGVGIDRLKPV
                     VGDHAFRHESGIHIAAILEDPETYEYFPPELVGTTRKFTLGKHTGKKALEHEVARLGY
                     HLPEKELCQVLEMVKDRGEKKVDLTDETLIDLIKQVMGGPPL"
     gene            complement(27552..28841)
                     /locus_tag="PKM87_03690"
     CDS             complement(27552..28841)
                     /locus_tag="PKM87_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNQ29201.1"
                     /translation="MKGVEALAHAILDATDRRYTVPGYPVTELGPMTGAEMVINEKTG
                     LEYALGDSLLGRRAAVIVKNVGVNACVDPLLQATAQGLVGGVLLVAGDDPDAVGSQTS
                     QDTRIIGELAEIPVIEPGSGSAFRAVEAALQASEEFSRVAMIRVTPGFLEGEATDSPV
                     PRKDSTGRLSDRSYTMHGRVMASRTLFRKMQEWADASPLNAWCGEPAGVGPEPGRSRV
                     VTVFPPPARLAEFRQVREAGLTFTKDHCRHEILPEEEGRAPPQTLEERGYYRTFCRDC
                     PFKPVISLMQDLGMRPVCDAGCVVLAMNPPYCIGVASYGMGSSVAVAARSTGVALTGD
                     YAFLHSGINALMDVYAKELSLLCVIFDNSCAAMTGKQPSLPVERYLGWADPHVVDVQD
                     IEEIRHQLQHAARPGVIVIRGSCPEGQEHEIVAYRDM"
     gene            complement(28838..29809)
                     /locus_tag="PKM87_03695"
     CDS             complement(28838..29809)
                     /locus_tag="PKM87_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ29202.1"
                     /translation="MRWNMLKARLLEAGAARLEGEPADGYISASTAGPGARGPGSVFF
                     SHGGRRVRLAIDPESSIRVMHRGGGEAVLSVDGKEISGILEKIALHCPRQAYITISSG
                     CIFSCRYCEVPGHPPRRKSVDEIVAMVEGVADRIDAISLTSGVMGSVEEEERYVLDVV
                     QKLGKFGIPIGVSIYPTNESAERLCALGVREVKFNVETATDALFEKMCRGLSRDDIWR
                     ALAQSVPLFGKNRVFSNLIIGLGETDEQVEECIQDLTRMGVIPVVRPLSPAAGCRGYR
                     RPSKERILSINRIHAEALCEAGLDPGAALTMCVACTGCDLVPGRDDT"
     gene            complement(29791..30708)
                     /locus_tag="PKM87_03700"
     CDS             complement(29791..30708)
                     /locus_tag="PKM87_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1743 domain-containing protein"
                     /protein_id="HNQ29203.1"
                     /translation="MRDPENAGQESLKDPYVIRYPEIVALSSRDGKQVELVEFFECVG
                     GAMWARLHYAKSPLVRSVRTVGGSTRYLLSPGQADLGLEGSRFPAGICGVEVSRDEIA
                     VSYVGLGGGGVGASGCRSLAGGVLRSECDPSGGGKKAGATLWLPRRERVLIGVDDTDT
                     PEEGATWTLCHNIASAVQDADSRYLSHTIVQLFPVPYRTKNCVAVVCEFATCDPARLV
                     SSFQRLLERYTLSEKTGMAVFTGFDPAPLGEYGWAVKRGEVNRERLAALKGRDLEYLM
                     EGRGIIGAVAAIPFSTRFEEALQLCDGTC"
     gene            complement(30984..31868)
                     /gene="fhcD"
                     /locus_tag="PKM87_03705"
     CDS             complement(30984..31868)
                     /gene="fhcD"
                     /locus_tag="PKM87_03705"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HNQ29204.1"
                     /translation="MQLNGVTIDDTYAEAFPTWIARVIITAVTEEWAKKAATEATGFA
                     TSAIGCPCEAGIESVLRPDETPDGRPGVSILICASKKKLKEQVVERLAECVLTSPTTA
                     VFNGLVGTDEKIPVKLHFFGDGHEYQKEVGGRKCWVIPIMEGEYVGEEEFGIVKGVAG
                     GNFFVMGENQMAALMGAQAASDAIASVSGVITSFPGGVVASGSKVGSNKYKFMPASTN
                     EKYCPTLREKVPDSKVPAGITALYEIVIDGLDEKAVSEAMAKGIKAAVMVPGVKFISA
                     GNFGGSLGPFKIELHKIL"
     gene            32291..>32525
                     /locus_tag="PKM87_03710"
     CDS             32291..>32525
                     /locus_tag="PKM87_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ29205.1"
                     /translation="MAFALHVNMDRCTGCNNCVVACPVDALELFTVDPATNEKIYKVR
                     HGKAMILDFKSELCAGCGVCVEACPHDVIRLAGR"
BASE COUNT         6288 a        10028 c         9597 g         6512 t
ORIGIN      
        1 tcccaagcaa caagtccaag ggatacggaa aacccttcaa cgagatcttc aaggaggcag
       61 gatacgactt ttacaagatc gatacttccc tcttctcacc cgcggaggtc gtcatcaacg
      121 agatctccga aggcgtggtc taccactgcg gcgcagtaaa tcccgaagtg acgctcaagt
      181 cattcgggct catctgaacc actcactctt ttatctcctt ccctgcacgt tgcatgcgac
      241 ccctctccag cacagaagtc cggaaacgaa gagaccacgg gtgatacccc caaaaagacc
      301 gttgggaaat gggaaacgac atgtcaggaa tggatgcctc ccgcgaggca tgggataccg
      361 actatgcaat gagggggcgc cggtgggcca ggggccactg caggatgccg tcatttcatc
      421 cgcacgagcg ggtgctcgat gccggctgcg gggacggaaa aggcctggca tccctggcct
      481 gcaacggtga cccttccaat atggcgggcg cacgttgccc ggaggtggtg gcttttgact
      541 tctccaggtc ggcaatcggc atctgcgctc accggttctc gcagaatgaa aaggttcacc
      601 tgctgaccgc agacgctgca cggatgccat tccgtgccgc gtcgttcgat gcagtcctcc
      661 tcttccacct catcggccat gcctcccgca ggcccaggag cgagatctgc gaagaggttc
      721 agcgggtggt ccgtcgcgga ggatacgtct accttcgcgt cttctcgcgt gcggactttc
      781 gggccgggag aggggaagag atcgaggaga tgacatttct tcgcggagac gggaccttca
      841 cccactattt tttgcctgag gagatcgcgg ccctgttccc gcaactcacg ctcatatcga
      901 tagagcacgt cccatggata ctccgggtga agggcgatcg tttccagagg gaagagatac
      961 acgccgtatt aagggcatga aaaaaaacct gcgacccatc cctttcatcg acgggtcagg
     1021 gcatggagat cacttgccat gaggggaaaa aaccgatgga tgagtttaat actctcaccg
     1081 ttgaatcttc caggcaaatc agggcccgtg gtctagtcgg ttatgacgtc gcccttacac
     1141 ggcggaggtc gccggttcga atccggccgg gcccattcca tctcttactg gcgaaaacgc
     1201 cgtcgtatat tcaccatgcc gggtgaagcg tgcatggtcg tttcttcacc ctgccggacc
     1261 agtgaggcat tcaatggttt tcccgcaccg ctcaattgtt ccccacaggt gtaatcacca
     1321 gtcactccca caacctatga tcggttcagt ttccatgatc ccaccggggt gtccctggat
     1381 atgcaatacg tcaccatttc tgcgtcgatt caccaaaaga gttaaataat gcaccctgcg
     1441 gcaccacccc agatttcccg gaaaaaaagt ccccatttat gcgttccatg gtgaatcggt
     1501 cttctcactc tgcctgaaac gatgagaaaa gcaaattttg acggaaatgt aatttctgat
     1561 taaaacgctt ttttgtggtt tttaacctct atacgcggtt ttttgtatta gcatgaccat
     1621 aaggctcccg atttgcgatt ccgtcataaa gcgtcagacg catgccaaaa gaaacgcgaa
     1681 ataatcactt tggaccatgg attttatata catgaagaag aaatggattt tttggtgcac
     1741 cgttcagcgg gcgacgcctg cctgaaaaag cactnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnngaaatg gattttttgg tgcaccgttc agcgggcgac gcctgcctga
     1921 aaaagcacta tacaatcaag gtggcatcaa tataggcagt actccaggct ttccaaagac
     1981 ggggcgcttc tcggtacacc agaatgagat atcacattgt gataaaaaag gtatcggatc
     2041 gcatcgggca gcccgccccg gtgccatcca ggtaacgcca tataaagggt tatttctgat
     2101 aatgctcctt tgaaagctct tcttttgggt atccagcacc gcccaggccc ggtgagaagg
     2161 gttgagacga ggtgggatcc ccgctctcat tgcttcttta caatctcttc gagggtgatg
     2221 gtgaagacaa gatcctttcc agccagcggg tggttctggt caatagtagt ggattccccg
     2281 gtaatgttgt aaaaggtgta atggaccacg acattcccgg gaaatgtata ttcaaagacg
     2341 ggctccatcc cggggaagaa gaggatccgg cttgtcccca tctggtcgag gttctggaca
     2401 aacgccaacg ctgtttcaat atccacctct tttacaagtg tctcattcct gaggccgtac
     2461 ccctcttcgg gagagattgt caccgtcttt gtttcgccgg ggttcatccc gatcactgcg
     2521 ttctcgaatc cctggatcac cgtgccgtct ccaacagtga actccagggg ggtcccgttc
     2581 acattgccct cgaatggttc agttccagga aaggagacag tataaaagac acggatggta
     2641 tctccggcct ttaccccgga agtggtgctg caccctgcga tgatcacgag cagcaacacc
     2701 atcccggaca ttgcaagcgc catgcggctt cccatcaatc ttttctgacg aggatcggaa
     2761 ctactcataa tctctccgta tacatttgat cggcaataat ccctgcggga ttgcctgaat
     2821 gagcgccccc tttattcact ccggtttgca ggaaaacggg agaattacgt tcccgaatcg
     2881 aattgtattt atcatgacaa ccctcacact ctagcatacg cgcgccgtgt gcgcggtgcg
     2941 ggagaagagc aaaatggcag agaatgaata caaggtggca gaggtggcag ggaagaaggt
     3001 acagtgggac cccgagcagc ttcggaagat acgtgagcat ccctgttttt cggagaaggc
     3061 atgccacgcg ttcgggcgat gccatgtccc ggtcgcgccg aaatgcaata tccagtgcaa
     3121 ctattgcatc agggactttg actgtgtcaa cgagagccgc cccggcgtga cctccaaggt
     3181 cctcaacccg caggaggcgg tcacactcgt gcgcgatgca atacagaagt ttccctatat
     3241 caaggtcatc gggattgccg ggcccgggga gcccctcgcg aatgaagaaa ccttcgagac
     3301 gctccgcctg ctgaaggagg agttcccgaa cgtcatcaag tgcctgagta ccaatggcct
     3361 tcttctccct gacaagatcg acctcctcca caagtacgac gtcggcaacc tcaccgtcac
     3421 cctcaatgca atcgatcccg agatcggggc aaaaatctac cagttcatcg attacaaggg
     3481 aaaacgctac gagggcgtcg agggtgcaaa ggtactctta aagaaccagc ttgaggggat
     3541 ccggatggca atagagcgca agatgatcgt gaagatcaat acagtctaca tccccggaat
     3601 caacgaagac catatccccg agattgccaa aaaagtcgga gagatgggag tgtacaattt
     3661 caacgtgatc cctctcatcg cccagtaccg gttctcccac atcacgccac ccacacccga
     3721 gatgaaagtg aagatgcagg acgagtgcgc aaaatacgtc cgccagatga ggcactgcca
     3781 gcgttgcagg gccgatgcag tcggacggct tggccaggac gtccagtcct gcctctatca
     3841 cgagtgaacg gcccccaccc catccctttt ttttgaaaaa tccttcctgc ccctgcacag
     3901 gggacagggg gtatcctttt tttcctctgg cgtggaagaa gacgtctatg cccgtctctg
     3961 aagagataca ccccgttgcc atcgccaacg agttccggca atgccgaaca tgcgggtacg
     4021 accgcggatt ccatacctcg ctgcatcgca tcgcggccgg gcacccccat ttcagagtgg
     4081 tgctgatatg ccccgaatgc ggaacaaggt atgatgcccg gtgggtaatg gagatctaaa
     4141 aaaaaagaga gtatttggca tctcaggggg ctgacccgtg ggtgccgaga aggtcgtcca
     4201 tggtggtgat gaccggcacc atttgtgcag ggacactcgt agtctccatg gtgatggtcc
     4261 ggttcccgac aacgatgaca taggtgatgt agtcggcccc ctgaacaggt gcggggtatt
     4321 catccttcag gctcgggata ttcgcatccc ggatcaggcc gcgaaggagg tcaacatccc
     4381 ggttttccag ggagaaaccc cctgttccat ggtgggtgga attgtacacc acccacccgt
     4441 cgctgaacac gaccatgtag tccgagaaac ccgcaatgcc tccggtcctt gaatattcaa
     4501 tcagcacccc ggggggactc ggcttctcgc ctgcaggtaa ggcttcatcc atgcaccctg
     4561 caaggagaat aaatgcaaga aggacacaga gtcctgagag cccataccaa gggattttct
     4621 ttctccccgg tgccggcaga gggttcgccc gcggaaaccg cctcatgaaa aaacagcctc
     4681 tatcttacat ctcaccggga aaataaagcc ttatccctca tcctgtgtcc tagtatagcg
     4741 accggaaaag agagggggcg cgggaccggc tggccgtccc caccggcagg agtttttaaa
     4801 aaaaccacat gattctcatc gagacataag aataaaaaat gaaaatcgcg ggtgcgattt
     4861 tcatataaaa agattgcctg ccccgggggt tgctgctgat tccccggcat cccgcaggtc
     4921 tgcaggcatc atgccagtca gttgataact ggcaaatcat cacccacaat ggagcatcgt
     4981 tgccgttgcc cggttatatc tccaggatat ccccggcacc gctcttctgg ctcttctttc
     5041 caggggtgat ctcctctatg ctcctgatca ggccgtcgac tttcgggttg gagaagatct
     5101 cgcggaacgc cgagagttcg ctggcactca tgatcattat ccctttcttc ttcatgggtg
     5161 ctcccttctc gttgacaggg ttgacctcga ttgcgaggga aggggcacgt gacttggtgc
     5221 cgggaagctt gatgatcgag acacctttga gcgaggtgtt cttcctctcc cagtcttccc
     5281 caccctcgag aaatgcccgc agtacttcgt tcaattccat agggaagaga tgtgggacag
     5341 gatataaaaa accctgcggt ttgcggggtg aaagagcaca tctgcggtcc catgtcttca
     5401 tggtcggggc atcggtcagg gagaccccct cttctcccaa tcgtatcatg gttcaacgcg
     5461 aaccccctgc ctgcagtgca tgtgccctca ccgccagggt ttagtgtgcc ggggacagat
     5521 cagacatgca cagaagtggt cccccgtgcc atccatttcg cttcgtcccg accagccgtt
     5581 tgacctcgac ctgaccctat cctgcggcca ggtcttccgc tgggagagag atgcccgggg
     5641 agtatggagc ggggtcgtag gagattccct tatccggatc tgccagcagg ggcagtcgct
     5701 cacattcgaa ggggcagaca ggaagttcat ccagacctat ttccagcttg aggaggacct
     5761 tcccgcgatc ctcggctcca tagaccggga cccgcttgtc cattccgcca tctgccggtg
     5821 ccgcggtctc cggatcgtcc gccaaccagg atgggagtgc ctcgcctcct acatcatcgc
     5881 tacgtttgcc aacatcccgg gtataaagcg gcggatatct cacctctgcc gctgtttcgg
     5941 gaaccctttc tttgggtcag gaggagagtg ctataccttc ccctctccag agacaatcgc
     6001 gttcagggag ccctgccgga tcgcggaatg ccgcctgggg taccgggcac cttacctcaa
     6061 ggagactgca cggctccttg ccggagatcc gggctgggaa gaacggctcc gggaactccc
     6121 ctacgacgcg gcgagacggg atctcctccg cctcaagggg gtcgggaaaa aagtcgccga
     6181 ttgcgtgctc ctctttgcat tcggcaaata cgaggcgttc cccgtggatg tctggatcca
     6241 gcgcatcctg gcgaccaggt atcccggggg agagtccctc cgcacgtatg accagtgcag
     6301 caggttcggg aggtcccact ttggggagta tgccgggtat gcgcaggaat atctcttctg
     6361 cgacagggca attcttgccg gagagggcag gggggatcag gtccccgtct cccagcctga
     6421 aaggtaatcc cgttgttctg gagtgagctc gtcgatagaa aggccgagcg atgcgagttt
     6481 cttcctcgca accgcatcgt cgatatccga agggacttcg tagacgccgg gggacatgct
     6541 ccgcccacag gagacgatat ggcgggcaca gagcgcctgc agggcaaagc tcaggtccat
     6601 gacctcgatg ggatggccca tccccttggg aattgccagg ttcaccagcc ggccctccgc
     6661 gaggacatgg atctcttttc cgtccacgcg gtaggtgtcg attccgtcac gccgggtcac
     6721 ggaccctgcg ttcgcctcca gccactggag gtcgatctca acgttgaaat gcccggcatt
     6781 cgccagcacc gcaccgggcc gcattacccg gaagtgccgg gcggtaatca ccgaagtgtt
     6841 ccctgtggtg gtgatgaaga tctcccccct ttccgcggcc gcatccattg gcatgacgag
     6901 aaacccgtcc atgtaggcct cgagcgcacg acgcgggtcc acctcggtga cgatgacccg
     6961 tgcgccgaga ccgtggagtt tgcgtgcaag gcccctcccg cagaagccgt atcccgcgac
     7021 cacgactgcc ttcccggcga tcagcatgtt cgtggtgagc atgatggccg agagtgcgct
     7081 ctcaccggtg ccatggacat tgtcaaagaa ccgtttcatc ggggtgtcgt tcaccgcgat
     7141 gaccgggaac ctgagcttcc catcgcctgc catggccctg agccggtgca ccccggtggt
     7201 ggtctcttcg cacccgccga tgacgtccgg gaggagatcg gtcctcgcgg tatggagacg
     7261 atggatgagg tccatgccgt cgtcgatagt cacctggggg cgggcatcca gcaccctgtc
     7321 gatggccgcg taatattccc ctgtcgtggc accgcgcttt gcatagcagt gcacaccttt
     7381 cacccgcaaa agcgccgcgg caacgtcgtc ctgcgtgctg agagggttgc aaccggtgat
     7441 atatacgtca gcgccgccgg ctgcaagcgt ctccacgagg cacgcggtct ttgcttcgac
     7501 gtggagggcc atgcctaccc gtatcccccg gaacggcatt tccctgatga actcctcgcg
     7561 tatggaggag agcaccggca tgaactgacg ggcccatgcc atcttcttct ctccggtctc
     7621 catggaacac accatccctc cggggagatc acaccagtcc ccggcatttc tcttaagaaa
     7681 ttccgtctgc agagtgataa aaaccccggg tcgcgcatgt cataatcctc ttatcccctc
     7741 cgcacccaca ctctttccat gacgcttacc cgtcggatca tcccctgcct tgacctgaaa
     7801 ggcgggaggg tagtgaaggg gacccacttc ctggatttga gggacgcagg ggacccggta
     7861 gagctggcgc aacggtacaa cgaacagggt gctgacgagg tggtcttcct tgacatcacc
     7921 gcgtcaaagg agcagcgcgg gaccatcatc gaggtgatcg agcgggccgc cgaccagctc
     7981 ttcctccccc tcactgtcgg tgggggaata cgctcggtcg aggatatcca gcagatcctc
     8041 cgcgccgggg ccgacaaggt gagcatcaat acgagcgcgg tgcacgaccc gtcactgctg
     8101 agcagggggg cagagaagtt cgggacccag tgcatcgtgc tcgcggagga cgtgaagcgg
     8161 aacttttccg acaatcccgc tgccaccccg gtcacccttt cggacggcag gacctgctgg
     8221 tacgaggtgg tgatctacgg agggagcaag cctaccggga tcgatgccgt tgcctgggcc
     8281 agggaggccg aggagagggg tgcaggcgag atcctcctta ccagcatgga gacggacggg
     8341 accaagaacg ggtttgatat ccccatcact gccgcgatat cggaggcagt gggggtaccg
     8401 gtcatcgcca gcggcggtgt ggggacactc gagcacttct ataacgggtt cgtgcgtgga
     8461 aaagcggacg catgcctcgc ggcaagcgtc tttcactacg gggagatgac cgtccgccag
     8521 gtcaaggagt acctagcccg gcggggtatc ccggtacggc tgtgaggtca atctccacgg
     8581 ccgcaacggg atgttcgagt tcgaaggcgt tcagcaccgc gggatggatc tccccaaaga
     8641 caccgatcgg acggccagcc gcgacaatgc tgcccctcct ccccccgatg aaggcaggat
     8701 cctccgactc ctccgaggta taggggatcg acaactccct gcacatcgcg tccgcaacgg
     8761 catacgcttc tgaaaagtcc gctgccgggt gaagtgagac aaaggccgcc ttctggaagg
     8821 tcagaagatc cctgaccacg tcaccgaccg caaagagccg ctgcggaagc tcgcgatgcc
     8881 ggttgaactg gagtgtctcg atgagaagag gaaggagccc ggtcctcacg atggtgttct
     8941 cctcgctgat agggtgaagg acggagaggg tttcgggagc aacggggcgc tgcatcttcc
     9001 catacatcac cttctcgctt gtgagcgtga atggaatcac ctcctggtac ccgaggcctg
     9061 acccgagcga acggacagcg gatgcaacaa tcatcaccgg gtgggcccgg ccgatcgtga
     9121 aggtcggcgg gagctctgcc ctgaagttct cgtacccgta cgcgatcgct gcgtcctcga
     9181 agatatccca gtcatgcatg atatcggccc ggtaacaggg aaccatgacc ctgaccttgt
     9241 catctcccgc gggttctgcc gagaaccgca tctttccgag gaggagggag agtccggcag
     9301 gggtgagatc agcgccgaga agggcgctgc actcgcgggc gctcaccagc cgggtcgagg
     9361 gtgccagggt gggaatatgg tttcccgcga catccacgct ctcgatccgg ccgccggcat
     9421 cggcaagggc cgtgcagatg atattcaccg cggtcattac cgcccgttcg tctgttcccg
     9481 tcgtatcaag gagaaggttt ctcgattcac cggtcacgcg ggtcagctcg ccgttgataa
     9541 tgggaggaaa cgagaggacc tggttgcagg catccacgat cagggggaac ttcggaaagc
     9601 ccctgacgat atcggcgtag gcgcgcccct tggggtgctc atcgaggatc tcctccatgg
     9661 tcatctctct ctcgtagtcc agaggcacga atgccctggt acgttcctcc gcgatgtacc
     9721 ggaatggcgg ggtcacgcgg tcaaggtcgt ggatccctat cgccaccttg ctcctgccgc
     9781 ggcccagcgc ccagtggagg gcctcctgga ggcccatcag cgagaggatc gcttcctcgt
     9841 caagttgtat tcccctgatg accgcggtgc cgagatacgg gcgtatcgag gcaagccccg
     9901 ggtcgaccga gaaactgatc ccggagggcg tgacatggta ctggggaagt cccgtctcaa
     9961 gcccgaggaa ccctcgcatc gccctcgcaa caccctcgac cgagaagagg tcggggcgcg
    10021 aggggaagaa ctccacatcg acatgatcct cctcgacccg ctcgatatca gaccctatca
    10081 tgggaaggcg cgcgagtatc gtctccctgt cggatcccac cagcctctcg aggtaacggt
    10141 aatacagcgt gatgactgcc atatcaggca cctcccctgg tctttccggg ctggtgccac
    10201 aggggcgtct cccgcacaaa gtcgatatcg gtcctgtaca gctgccggag atccttcaat
    10261 cccagccgga gcattgcgac ccggcttacg ccaaggcccc atgcgagcac gggatgggtg
    10321 acgccccacg gtgccctcac ctcttccctg aagatccctg caccccccat ctcgacccag
    10381 cccaggccgt cgacatacac ctcgggttcc accgacggct cggtataggg gaagtatccg
    10441 ggcctgaacc ggacgtcctc gaatcccatg cgaccgtaga actccttcag gaacccgagg
    10501 aggtggcgga acgtgacgtc ccggtccatg acgattcctt caagctgctc gaattcggcc
    10561 aggtgggtgg cgtcgattga ctctctccgg tagaccctgc cgatgcagaa gacctttacc
    10621 gggggctcgg gatgcgcaac aaggtgctga atggagaggc tcgtcgtgtg ggtgcgcagc
    10681 acgcactgtt ctgccttctc cctgttccac tgtcctcccc accctgtcga ggaggtggtc
    10741 cctccgtgga ggtgcatgtc tctcaccctc tcgaacccgg ccgggagcga aacggtctcc
    10801 ttcaggaaga acgtgtcctg catctccctg gccgggtgat cctgtggctg gaagagggca
    10861 tcgaagttcc agaacgaact ctgggcgagg cccccgtaga tctcggagaa acccatctcg
    10921 aggaggatct ggcgcatctc gtccaggatc ctctggtaag gatggacttt cccggggaag
    10981 accttccggg gcagggtctc aaggctgtat cgcctgatgc gaagatcctt ccaggcgccg
    11041 ctcttgatct gctcgcgaga gagagttccc acctcctcct cgaggtcaag ccccttcgct
    11101 gcgagggcct cgccctcggg ggtcagggat accaggtagc gtgtctcctg gtgctcggtg
    11161 agaaggcccc gcttctgcag gatcgcgacc ccgtcgccac ccctggatag gtcagagagg
    11221 gcgacctcgt cctccccggg cgcgtcccgg ccggtcttct ccaccacccc gtcgcagacc
    11281 ttcacccacc ccttttttcg catctggcct atcccgacac gggataatgg atgcgtggtg
    11341 agttcggaca tcgggacccg ctccccgaaa ctttcaagca gctggcgctc gggcaggcca
    11401 tccctcgcat accgggctcc ctcctctgtg agatggtatg tggtgtcgac cttcttcatc
    11461 acattggcaa ggccgcgatt ctccagcaga aaggcaaact ggatcaccga ctcggcggtc
    11521 gtctccatgc gggccgcaag ggtggccgga tctgctcttt tcagcggttc aagaactctc
    11581 aagagtcgtt tttcattcag tgacaggtcc atctcactcc accttcacca ggtgtgccac
    11641 agcctccatc ctctcccgga agtcccggac aaaggccagc acccgttctg cggtctcctt
    11701 cttgcactgc ccgcacatca gctcgcccgc gaggcagcgc ctccggatct cggcaagctc
    11761 ggcatcgtca tccaccatgt ggaagaggtt caggaggtag aggggacacc tgtcgggctc
    11821 accgccctgc tctttctgct ccgcgagcgt cggccggcct cccgtgaggg cagccatcac
    11881 cttctttctc accacctcgt ccggctccgt gaaggtgatc gtgctctccg gtacactgct
    11941 cgacatcttt ccgccctgga ggccggggat gaagcgatgg taggtcgacg agggagtgaa
    12001 aaagccatac ccgccatggg cgatctcgat attacgcacc tcgacgctca catcggtgca
    12061 tctccctccg gggatatcga cgtgaccttc atacttttta gatccggcaa atctccggtg
    12121 cacctccccg agggctgcgg gaggggcatt ctttgaccga acactgatgt atccgtccct
    12181 ctcttctacg gtgaacatcc tgagcttgtg ggcgaccccc ctggtcaggc ggatgtgcgg
    12241 gtcctggtcg atgccgaccg ggacgatggt gggagccggg gggctgtcca cctggggata
    12301 gaggatatcg gcaacctggg tgatcacgct ctcggcatgc gcaagtgcgg tctcgtccga
    12361 gaacccgtag attgcctgga gttcagagaa gttcaccttc gttgcggcct cgaaggcgag
    12421 gtccatcagg cgcttgttgc ggctctggaa ataggtgtgc ccctggtagc ccagcgcatg
    12481 gagacactgg agatactcct tcccgtactc gtcgcacttc tgccagggga tatcgcgcac
    12541 cgcgtgggct tcccggtcag caatggtaat ataccctgtc cctccctgct gcacgtgcca
    12601 taccacctcc ttcatcacca tcaggtggcc gagatgcggg tggccggaag gcatgaatcc
    12661 ggtcaggaca tgaaagcgcc ggttgtccct gatggcctcg gcgatctggc gataatcccg
    12721 gtggcccacc accacacccc ggctgatgaa tgacggtaca tgggggagga tgccatcgat
    12781 gctgctgata ggttcgatgc cgaacattga gaagagcttc tcgatatcgt cggactgctg
    12841 gctggaccag gggtttaagg gtgcctgcat gcgttatcta ctcacagttt aatccgggca
    12901 aattccacgt attggattcc ttgactatgt tcgcacccga acaacatctt ctttttcaca
    12961 ctgtgagcca ggcggacgga cctcgatatg gcgctcatcg gaaggcttgc gcctgcagga
    13021 agggcctgca ccagcatctc ggaatggacc ttctttcctg aatagacacg gaagtgatgg
    13081 ccgaacttgt agccggtccg gggtacgaac ccgcggttcc tcatgtcttc gtagaccgca
    13141 accttctccg ggaactcgat gtcggactcg tgcgccatcc cgtaatagcg ctcagggtca
    13201 atcggttctc cgttcctctg cagcgtgagc cggcccgagc gcatcaggtt caggatctcg
    13261 agcggagcga gcacgaggcg gcccgggtca agctgcatac cgtaactgcc gggaaccgcc
    13321 gacccggatg gcgggacatg gacaatcgca tacttcccga ccagttcgcc cctggtgttc
    13381 cattcctcct cctccttctc cacctgtggg agtgtcggca tcttcacctc gtaataggtc
    13441 agctcgttct catcatcgac caccgcaagg acgtgctgct ttcgcaggtt gagcgaggct
    13501 gccgcctctc cgagaagccc ggagaagtcg atcaggtccc tctcggagat taccctgacc
    13561 aggtactggg actggcccgt tccaggcctc tctcccctcc ggaagacgcg gaagtcatgc
    13621 gggccggtct gcacgacgta tcccctctcc cggatgtcac ggtacaccag gtaactccgc
    13681 atgaattcgg gcctctctgc gcagacggcc aggagacggt cgaatgaata ccctgcgacc
    13741 tcgagccttc cccgatgaac aagatagagg gcctcttcgg gcgcgagccg tattccctcc
    13801 ttttccggcc tgccataccc gctctggtcg tagagtgccc gcccctcgtg gcctgcccgc
    13861 acccacgtcc cgtcaaaagt cgctttcact gtatacccat tggaagaatg cgctcataat
    13921 atcatgccgc gatgcagtat gccttatgga acaggattac cccaaaaaca ggcatcagat
    13981 cactcttata gaataccagg agatatgccc tgaataccct tttgtgggat atatataact
    14041 cctttttgca tttcgaagag attctggctg cgcgaggggg ccaggttgcc aggggaattg
    14101 cattacttat gtaatacaat tgtagtactt tttcgcggaa aaacggccgc agagagaagg
    14161 acttatacca ttccggctgg aaacctctct tttcatgtat cagggggaga ttgccccggg
    14221 ggaatgcgat ggcggttttc gaccaggcgg ggtgaagggg atatgatcaa ttgtgccggg
    14281 tttctcgagc gatccgcggt gaacgggccc gggagaagag cggtcgtatg ggtccagggg
    14341 tgcccgctcc gctgccctgg ttgtttcaac cgggaccttt ggtcattcag gaaggcaaat
    14401 ttcgtccacg aggagatgct ggcggagcgc attctctcca tccccgggat cgagggggtg
    14461 tcgttttccg gcggggaacc gttctgccag gcagcccccc ttgcaacact cgggaaaata
    14521 atccgcgatg cgggcaggtc cgtcgtgacg ttcagcgggt atccgataga gaatctcctg
    14581 cgaagccggc ggcaggactg gagggcgctc ctgcaggaaa ccgatcttct cgtggcagga
    14641 ccctacctcc aggaactccg catggaatca tctctttgtg cctcttcaaa ccaggtggca
    14701 gccttccttt ccgggaggat cactccttat caagaagagg cagacaaagg agagtcagtc
    14761 gagttcacca tatgccgcga tggggagatc accaccaccg ggttcccgaa gagggcccct
    14821 cttcctttcg agcgggggtg cacctgatcc atgtcgcact tctcaaaagt gaagaccgcc
    14881 ttcaaggacc agtctcttct cgcacaatgc ctcgaggaga tgggctacca ggtcgagaca
    14941 ggggggacca tcaggggtta ccatggcgtg cagcaggttg acctctctgt tgctggaaga
    15001 gacgggtacg gcgtggggtt cgtccggaac ggagatggct gttacgatct cctggcagac
    15061 tcctggggag tgaagggtgc agataaaaaa atcctcgatt ccctgcgccg gaactacccc
    15121 cgaatccagc aggagtacgc ccggaggacg gtgcgtgagc aggcagaacg tgaaggatat
    15181 accatggtag aagaggttga gcaggaagac ggctcggtcc gcatcgtggt ccggagatgg
    15241 gcatgagaac agatgccagc gagcaggata tcccctccca ggtcgacctc tgcctgaggg
    15301 cacgcatcac cttgctcatc ctgcacaccg ccgaggagga gagggcgatc aggatgctcc
    15361 aggatgtctg cggccgcttc tcccccaaaa ggcctttttt cacctgggac cattcagacg
    15421 ggtttgcggg gccggccggc acaccctgca ggggctcttc tgcgagagat cccctcgtag
    15481 ccctcgaaga gatggagaag accgacgaag cctccgtgat cgtgctcaag gacttccatg
    15541 agttctgggg aaaccctggc gtgaagcgaa ggctccggaa cctggcccag aagttccggt
    15601 tctcccgcag gaccatggtg gtggtcaccc cgtcattgaa ggtgcccgag gagatccgtg
    15661 acgatgcggt cgtgcttcac gtgcccccgc cttctgcgag ggatctcgag cacgaactcg
    15721 accggctgct gcgcgggagc ggggcaggag attccctcac ccccctcggg cgggagaagc
    15781 tgatccaggc agccctcggg atgtccctca accaggcctg caggtcgttt tcgaaggcga
    15841 ttgtccgccg cgggcgcctc gatgaccgcg acatcgattc agtggtctcc gagaagaagg
    15901 acatcctctc ccagagcgag gcgctcgagt tctacagcgc cacagagacc gcagagaacg
    15961 taggcgggct ttccgtgctg aaggagtggc tccgcctgag ggagcgggca ttctcgcgcg
    16021 aggcccggga gtacgggctg ccgtctccga aggggatcgc cctgataggc atccccggga
    16081 ccggaaagag cctcacggca aagatgattg ccgggatgtg gaggctcccg ctgctccgcc
    16141 tcgacgtggg agcgctcttc gggagctttg tcggggagtc tgaggagcgc acccgccgtg
    16201 cacttgccct tgcagagacc atctcgccct gcatcctctg gatcgacgag atggagaagg
    16261 cattttcgtt cgggaacggt gactccggga ccagccagag ggtctttgcg accatcctca
    16321 cctggatgca ggacaagacc tccccctgct tcgtggtggc gacggcaaac gacatctctg
    16381 cgctcccccc ggagctcctt cgcaagggga ggtttgacga ggtgttcttc ctcgacctcc
    16441 cctcgcttgg agagcgcagg gagatattct cggtccacct gcggaggcgc ggactccttc
    16501 ccgacgactt cgaactggac cgcctggctg cggcgtccga gggttacgtg ggtgccgaga
    16561 ttgagcagac ggtcatcgat gcaatgtacc atgcattcaa ccaggatatg cggcgggtca
    16621 ccaccggcga catactcacc gcgctggggg cacaggtgcc gctctcgatc tcgcagaagg
    16681 aagtgatcgg gggacttcgg gcgtggctga ctgaggggcg ggcactgtcg gcgtctccac
    16741 cagctacccc tgccgggatt ccgggcggat cgatctgcct ggagccggtc gtctcccctg
    16801 ccaaccggtg aatacccatg cccgcgatgg accggatgat cacccgcgtc tcctgcggga
    16861 ccccgctgat cgggaggcta atccgctcgt ctgcaattac agggctcgcg gtcgcggcca
    16921 ggtgtgggga caatggtgca gtacgtgcac tctcgcgggc atttgtggat tcggtccatc
    16981 ctgatgtgag ggatgcagca cgggagtgcc tctcctccct tgaatccccg gacgccctcg
    17041 aagccttctg tgactgcgta ctcggggagg gggacccccg cctcatctcc atcgccagga
    17101 aatgcggata tctcccccgc agcgatgaga gaaaggcact gtttctcttc ctcaccgggg
    17161 agattgcggc ctggcgggaa ctcgatctcc tcgaaggtca ccctttgctc gaaaagtcgc
    17221 tcctctctgc accgccggat atccgttcga gggcgctcgc acaggccagg gagcagggaa
    17281 tcgcacgcct tttccacgcg ctcgcagacc gccctgatgc gcccgggtgg tcgactggag
    17341 actggagcgc acacttcgat ttttgcagaa aggagaggcg atgggatgaa ctgtatgcac
    17401 tcctcttcct cgcaccattg ccatacgccg tggacgcagt ccaaacgctc cgtgtatccg
    17461 ggtacaagcc cccggacaca gacctcgcgc tctggaagga cctccttgcc ctggtcccgg
    17521 accgctggag ataccctgaa ctcccggagg caccatccgg gatgctttca ggccccggcg
    17581 gcagggtcga gcaggcggca ttctcccctg atgggacaca ccttgcgacg cttggttgcg
    17641 acggcacact ctgccagtgg tccctgcccg gaggagtcct cctctcttca tgcagaccgg
    17701 catgcggagg catcaccacc ctcgcatacg ccccgggagg acttgccctt gttctggggg
    17761 gaaacgacgg gcgggtgagg gtgctcggga cagggaacag cacggagatc cgcgagtggc
    17821 aggcccacgg cagcgcagtg gccgcgattg cctttcctcc cgaaggcgac acccttctct
    17881 cggcaggttt tgacgggact gtctgcacct ggacctggcc tggtggagag gtggcggacc
    17941 gaaggcacct ccatgattgc gccgtcgcag cccttgcatg tcatggcgac ctggttgcct
    18001 ccggtggcag ggacgggacc gtgatgctct ggaagagagg cgatatggaa tatgcggaga
    18061 tccagggggg aggagacgcg attcggggtc tcaccttctc ctgcgaagga gcggccctgg
    18121 ccgggattga cgagagaggc acgttcagga cctggaactg cgaggatggc gctctccgca
    18181 tgctcacgcc gtcccatgca aagcggttca tcgcatggtg ccacactccc tccggggact
    18241 tcggggcgat ggccactgac gaacacaccg cggtgctttt ctccccctcc gaagccaggg
    18301 aggtgctccg cttcgatgtg ccggggcagg gaatcacgtg ccttgcactc cacccggggg
    18361 gagcctttct ccttgcaggt tgccgggacg gatacctcca cacctggtca atccctgagg
    18421 gaaagagact ccactttatg aaggcccacc agggacaggt ccagctcctg gcagtcaaca
    18481 gttcggggac cggggcgatc agcgcggggt gggatggatc ggtccggctc agggcgctgc
    18541 ctggcggtga actgcagacc gtcatgcagg gccccggcgc tgctatccgc tgccttgcgg
    18601 ccaccccatg cggagaagtc ctctcctgcg ggagcgaggg cgggattctc atggtgtggg
    18661 agacacgatc gcaggaactg gtgcactggc acaacctctt ctgcggccgg atagaatgcc
    18721 tcgcactcga cccttccggc accctggccg catgcgggga tgcgcagggc agggtctcgc
    18781 tctgggatat cgagaccggt tctcttcggg caaccctgga gggacacgag ggcggggtgc
    18841 atgcccttgc cgtgagcctc gagggggatc tcctcgcgag cgcgggctgg gacggcgttg
    18901 tccgcgtctg gtcgcttccc gaagccaccc acgtcgcatc ccttgagggg catggaagtg
    18961 ccgtcacttc cctttcattc tcgccggcag ggcgcttcct cgtcagcggt tcacacgacc
    19021 ggtctgccat cgtgtgggat ctggaatcgt gcgaaatgca tgcccgcatt tccggccact
    19081 cgcacgtggt ctcctgcctt gggatttcgg gcgatggccg tatcctcgca accgggagct
    19141 gggaccgggc aatccggctc tggagcctcc catcaggtgc gccgatggga actcccctca
    19201 tacatgccgg gagggtccgg tccctcgcgg tgcaccctga cgggaatttg ctcgtgagcg
    19261 ccgtcgatgg cgggaccatc tccatctggg cactccccgg aggggagctc atccgcaacc
    19321 gcgtggtcag ggccgatgcg cgcaacggca tctgtatcat ccccggtatg cactgggtgg
    19381 cttccgcagg cctggacgga tcacttaacc tctccgggct cccctgggca aaaccgatga
    19441 gccggaccac ccccgccgat attggatacg tgcagacatg catcagccgc gcttcctctc
    19501 atcaggatat ccggcagtgg aaattcctcg agcgcctcct ggccggcagg ttccgccagt
    19561 gcatcgcgta tggcggtgcc gggatgccgg ccggcccgca tgatattgag attgtcgaag
    19621 aaccagtgag atccgaagcc gtcgcgtgcg gggtgaggat gtaatgccac tcagggtctc
    19681 catcgatttt ggcacttcga acaccgtgat tgcatgctgg gacgaaggcc agggatccgc
    19741 acgggtgcgg gagattcccg ggtggtcccg cccctgcaat gcagaaccgc gaagcacccc
    19801 ggtgatcccc tcccttatcc actacggcgg ggaagaggcc cggtggatcg gacagcaggt
    19861 gcatgaccgc ggcctcctcg attccccttc caccgttcgc tggatgaagc actacatctc
    19921 caccaggagc ccggtccggg tcccgctgcc cggagggagc aggagttacc gcgaggtggg
    19981 tgccgatttc ctccaggcca tactctcatc catcctgcct gcacagggtt gtgaggagat
    20041 cgaggctgtc ttcccggtgc cggtagaatc gttcgagcat taccaggact ggatcatctc
    20101 ccttgccggc tccgcgggaa ttggcagggt ccgggtagtc gacgaggcga ctgccgccgc
    20161 ggtggcgtcc ggcttcacgc tcatacccgg ggaggtgttc atgctctttg acatgggggg
    20221 cgggaccctt gacgtctcgg tggtccgcct cgaggacaaa ggtgatagtc ccggccgccg
    20281 ctgcagagtg ctcgggaaag cgggggatga tatcggcggg atgcggatgg accagtggat
    20341 cttcggcgag gtatgggaga ggtgcatgtc cacctgcgat gagcggtcga gaaaggcgct
    20401 ctcgcgtccc ctgctctcgg catgcgagag ggcaaaggag gcactatccc ttggagagga
    20461 gacggaaatt tccgcgtgca ccggcactca tgaccggtgt gaactctcga ttccctacac
    20521 gaggaccgag ttcgagcgcc tcatggaaga gaagggggtc tttccccgcc tcaatgcagt
    20581 tatccagagg gcactccagg gggcctattc gcggggtttt gcagaggacg accttgccgg
    20641 cgtggtcatg gtgggggggt gcagccagat cccggccata aaaaaagcgc tcgagtaccg
    20701 gttcgggcgg cagaatgtcc ggcacgccca tcccctggag acggttgccc ggggagcggc
    20761 agcgtgcgcg gccgaaattg accttgacga ccatgtccag cacgactatg ccctcaggtt
    20821 ctggaacccg cggagcgcag agcatgaata ccgtatcctc gtccggcagg gtgacccgta
    20881 cccgagcatt cgccccgtgg cccgtctcct cgtcagggca acctatgatg gccagtccca
    20941 gttgggagtt cccatcttcg agatgggaaa agccaggggt aatgcccggt cttcgcagag
    21001 ggaactcgtg agcgagccgg ggggcggggt ccggctcatg gacatcccgg acgatgaaca
    21061 ggccgggacg cacgccttct ggatgaacga gaatgccccc ttcttcatca cggcgaatcc
    21121 tcccgccgca agagacgagt ccaggttcga gctctccttc tcccttgacg ggaacaagag
    21181 gctccttgtc actgcccgtg acaaccggac ggggcaggtg gtggtgaagg accagccggt
    21241 ggtgaggctg gtatgaagtg gtgatgcaaa atggagatgc aggaacttga gatagtgatc
    21301 gggcgggacg gccaggtggc cgtctccgtg aaaggcgccc gggggccagg atgcctggcc
    21361 ctcaccaggg cgcttgaaga gcggctcggc gacgtggttg ggaggaccca caaggcggag
    21421 ttctattcgt cgtcagaagg tgcaatggtc caggaacgcc cgctggaaaa tgtgggcgaa
    21481 aggagggcgt aaaccacata ctgcgatatg atggagcata ggtttagaga caggccccca
    21541 taaagacagg agggggttca ccctccctgg actctccccg cgcacctccc atcgcaaggg
    21601 gacttttagc cccttctgct cccggcaagc tccctcacct tcgcgatatt ccccctgtcg
    21661 tcacgcctct catcgacagg tgtctcgcag atcaggggcc ttgtgcggaa aaagtcatcg
    21721 tggagcatca gggagaacgc ctcttcgcct atggagccga ggccgatgtg ctcgtgccgg
    21781 tcgagaccgc tcccaagagt ccccttcgag tcgttgaggt ggatgagacg gacgtgtgca
    21841 agcccgatga cctcctcgag cctccccaga gtctctgcga gtcccttgga gttgcggagc
    21901 tcgtatcccg cgccgaaggc gtggcaggtg tcaaagcaga cccccacccg ctcctggcgt
    21961 gatatcccgt ccatcacggc ccggatgtct tcgaatgtgc tcccgacacc gttcttctct
    22021 cctgcagtat tctcgaggag gagcatgacc cggttcggtg cggcctcgag cgctttgttg
    22081 acggcatgga tgacccgggc cctcccggcc tcgatgccgt caccgagatg atgccccagg
    22141 tgggtgacga ggaacgggat cccaagcgtg ccgcagcgca ggagttcggc agcaagggtc
    22201 gcgcacgact tttcatacat gtcctctcgc ggcgttgcaa ggtttgggag atagggcatg
    22261 tggtcaaaca ccggggagat accggatcct gccagggatt ctttgaactg ctccacgtcg
    22321 ccgggagaga ggtccctgta cccccatccc cgcgggttcc gtgagaatat ctggaatgta
    22381 tcgcagccaa tgtctgcagc acgctccacc gcaagggaaa tagaccctgc gatggagacg
    22441 tggactccaa ccctgaccat gcgggctcag ttcctcccgc aattaccatg cgacatacca
    22501 tctgatcggg acccgcgggg aaaaaaagga ttggtacggg aaggtggccg tggacagaac
    22561 tggcaggacg ggattatttc ccgaccccgg cgaggacgct gttgatctcc tggaggaccg
    22621 cctctttctt gaacggcttg ataatgaacc ctgctgcccc cttcttcacg acgtccctga
    22681 tggtatactc ctggtggtcc ccggaacaga tgatcacccg ggtggagggg ctgatcttcc
    22741 ttatctcctc gagcgtctgg ggcccacgca tcttgggcat gatgacgtcc agtatgacca
    22801 cgtcgggatt ggtctccctg accttctgca gggcctcgac cccatcgctc gcctcgccca
    22861 ctacctcgtg gccgcctgat atgaggatct gcccaaggag gtaccgggtg agctcggaat
    22921 catcgacaag gaggactttt cccataatct ctatgtctct tttggggact cattatataa
    22981 aacatgcccg gaaacctgca tgtttaacgc cggatcaatg attccaattc ccggataatt
    23041 tcgtttccga actcgactgt cccgtacatt cctcccatgt ccctggtcct gacgtctcgt
    23101 gcacgcaccc tgttgattgc cgtctcgatg agcctcgcag actccgggtc gtttgcgcgg
    23161 tgcatgagca gcatcgaggc gctccggagt gcagcgatag ggtttgccag gttcttcccg
    23221 gcgatatcgg gggcgcttcc gtgcaccggc tcaaagagcg cgtgcctctc tccgaggttt
    23281 ccgctcggca ggagcccaag accccccacg agatatgccg ccacgtcgga gaggatgtcg
    23341 ccgaacatgt tggtggtcac gatgacatca tacctttcgg ggtgctggag gatgtcgagg
    23401 cagagggcgt cgatgaagca ctccttccag ggcactccgg cagcgtttgc ctccgcgatg
    23461 cagatcccgc ggaagaagac atcggacttg aggacatttg ccttgtgccc gatggtgagg
    23521 ccagtcctcg accgggcaaa accgcatgcg aacctcgcga tccgctcgct ccccttccgt
    23581 gtgatgcagc gcacggtgca tgccgagtcc tgctcgatct tctccacccc ggagtacagc
    23641 ccctcggtat tctcgcgcac gaccaggatg tcgaaccctt cgccccacac aggccggagg
    23701 ttcgcgtaga ggtccagttc tttccggatg cgcacgatta cgctccggta atcgggcgag
    23761 gggggagtgg tgaccgcacc aaagaggatg gtgtcggcgg acgcgagagc atcgatctca
    23821 tcttccgtga tagcctggcc tgtccgctcc cacctcccgt atcccacctc gaccgggaag
    23881 aactccatgt caggccggag gacttcaagc gccctctttg ccaccgggat gacctcgtgc
    23941 ccgatcccgt ctccctcgac gaccgctacc ctcatgccct ctccctccag cgctcgagga
    24001 ggcggcagac cgcatggtag atttgcgcag ggttcgcacc ccagtggacc accgcgccaa
    24061 tcttcccgtt atagaccccg accccctccc gttccttccg gcagcagcgg gcaataaacg
    24121 gttcatcttt taccatcttc agcgggcaga tctcgactat gtcgaaatca tccccgtagc
    24181 attcccggat cagctgggca ccggtcatgc accctgcaac cctctctcct ccctctaggg
    24241 ggtcggcgtc cagggtgtgc gtgaatcctg ctgccctgca cgggtacacg tctgcctgct
    24301 gcagggatat gtcctggagt gaatgttcga acacgacatc gagatcgccg aacagcccgg
    24361 ttgcttcgag ttcacggatc gcggacgaga ggctgggccg gggaggtacg acatcataga
    24421 catgcaccgt ctggaacgca ccgatgtcag ggtcgagcac gaacgtggag tgctcgtcga
    24481 ggccggtgaa gatagtgcac cgggtgcccg attccattgc caggcggacc agcagggccc
    24541 gatcgtggat ctggaccttc tccgggtaca cggtcacctc tccgggagca gcgagcacgc
    24601 actcattcct gatagcccgc atcaggccgg gccctttttc gtccaggtgc acctccagga
    24661 gctccggccc gtcttctgtc tccctgacga ggaaccggga gagaaaatac accttgtccc
    24721 cgcagggccg ggaagacgcg tatcccacct ccttgcacag gcgggggaag atcacctccg
    24781 gcccctccag tacgagacca gccctccggc agagaggatc tcccgcatcc gtggagagag
    24841 cggccgggtc aggtaggacc ggtccccgat tgccaccttc cctgcggccg gatccgccct
    24901 caccatctcc ccatcggtgc aggtgcattc gcactcgatc accgggaggc cgacgttgat
    24961 ggcgtttcta tagaatatcc gggcgaaact tggtgcaatc acccccctca cgcctgcttc
    25021 cttcagggcg atcgcggcct gctcccgcga agagccgcac ccaaagttct tccctgcgag
    25081 gatcaccgtt ccttccaggc gggaggagag ggtagggtcc aggtcttcga acgcatgccg
    25141 tgcccattcg gacctgtccc tcgtgcgcag gtagcgcccg gcgatgatca catcggtatc
    25201 gatgtccggc ccgaggcaga gtgcatgccc ctcgatgatc atcgtaccgc ccccgggctt
    25261 gcgagcgccc cggccagggc acttgcagca gcagtggaga ccgagccgag gtagatctcg
    25321 cccccgactc ccatgcggtt ccggaagttc cggttcgcgg tggagaggca gacctctccc
    25381 tcccccagca cgccctggtg tgcaccgagg cacgggccgc agcccggagg gagaatggtg
    25441 cagcctgacc tgaccaggtc tgagagcacc cccgatccgg tggcccgtgc aaggacgttt
    25501 cgcgaggcgg gtgcgatgag cgtgcggacc ttcaccctct ttcctttcac catcgccgag
    25561 aaccgctgga gatcctcata cctcccgttg gtgcaggtgc cgacgaatac ctggtcgagc
    25621 gggagacctt ccatctcctc cacgggccgt atggtatcga cccttggggg aaccgcgacc
    25681 accgggacga ccgcggacat gtcaatgtgg agctcccgct cgtagcagca ctcgcctggc
    25741 tgctgtggcc tggcgtggtg gccgtaccct tcgaggtacc tgcaggtggc accgtcggcg
    25801 taaaagagac ctgcctttgc ccccgtctca accgccaggt tggagagcgt catcctggag
    25861 tcgatggaga gggtggctgc cccgtctccc acgaattcga gcgcccggta ggtcgccccg
    25921 tccatggtga gccttgccac gtaggtgagg gcgaggtctt ttgcctccgc ggggagagcg
    25981 agcgccccgt ggagcctgat tgcgatggtc tccggcacgc gaaaccacgt ccctccctcg
    26041 gcccagatag ccgccatgtc cgttgcgcct acgccggtgg caaatgcccc gaatgccccg
    26101 agagtacagg tgtgcgagtc cgcccccacg atgatctcgc ctggaagggc aatcccctca
    26161 cccatcagct ggtggcagat cccctccccg acatcggtga agttgaggcc atgctcccgg
    26221 gcaaacatgc ggagatcttg ctgcaatcct gcggtgaccg agttgtttgc agggacgatg
    26281 tggtcgaaga ggacataaca gcggtcgggc cttgccagga cccctgcctg cattgccctc
    26341 catgcctcga gcgtgagcac ccctgttccg tcgtgcacgt aagatcggtc tactgggcag
    26401 tccacgtagt cgcctgcagg cgctccgagg atttgctccg aaagcgtgct cacagtggcg
    26461 gcccccccat cacctgcttg atgaggtcga tcagagtctc gtcggtcagg tccaccttct
    26521 tctccccccg gtccttgacc atctcgagca cctggcagag ctccttttcc ggcagatggt
    26581 acccgaggcg ggccacctcg tgctcgagcg ccttcttgcc ggtgtgcttt ccgagggtga
    26641 acttccgcgt cgtcccgacg agttccggcg ggaagtactc gtaggtctcg ggatcttcga
    26701 ggattgcggc gatgtggatg ccgctctcgt gccggaaggc atgatcgccc accactggct
    26761 tcagccggtc gatcccgaca cctgaataat ggacgaccat ctccgagagg gggacgaggt
    26821 gggagaggtc ataacgcctg actccgccct tcatgtgaag tcccacgagc acctcctcga
    26881 gcgccgcatt cccggcacgc tccccgatgc cgttcaccgt ggtgtggagc tggaacgccc
    26941 ctgcctccgc ggcggcaaga gtattggccg tggccattcc catgtcattg tggcagtggg
    27001 cgcagagtgg ctttcccacc gccctgacta tcttccgcat tgtccggtac atctggaggg
    27061 gagtgagtgc ccccaccgtg tctgcgaatg agagcaggtc tgcaccgtgc tcctcgcccc
    27121 tcctgtacac ttccatgaga aacggcaggt ctgtcctgga cgcgtcctct gcagcgaacc
    27181 tgacctggag gccatggtca tgggtataat ccaccatccg aagtgcctca tcgagcacgt
    27241 tctcccgtgg ggcatggaac ttgtgccgga tgtgcagttc cgatgtgggc atgaagatgc
    27301 tcaccatgtc gacgccggcc tcgatggccg cgtccacatc cgctctcctg gcccgtgcga
    27361 ggcagcagat gcgggcatcg agcccgagcg aggcgacggc gctgacgcac cgtttctcgt
    27421 acggcgaggt tgccgggaac ccggcctcga tgacctccac cccgatctgg tccagtacct
    27481 ctgcaattct cgtcttctcc tcgcaggtga aggagactcc aggtgtctgc tccccgtcgc
    27541 gaagggtgac gtcacatatc tcgatatgcc acgatctcat gttcctgccc ctccgggcat
    27601 gatcccctga tcacaatcac tccgggccgc gcagcatgct gcagctggtg ccggatctct
    27661 tcaatatcct gtacatctac cacgtgcggg tcggcccagc caaggtaccg ctcgaccggg
    27721 agggacggct gcttccctgt catcgcggca cagctgttat cgaagattac gcagaggagg
    27781 gagagctcct tcgcgtacac gtccatcagc gcgtttatcc cgctgtggag gaaggcatag
    27841 tcccccgtga gggcaacacc cgtgctcctg gccgccacgg ccacgctcga tcccatcccg
    27901 taactggcca cccctatgca ataggggggg ttcattgcca gcaccacgca cccggcatcg
    27961 cagaccggcc gcatcccgag atcctgcatg agggagataa ccggtttgaa cgggcagtcg
    28021 cggcagaacg tcctgtaata cccgcgttcc tcgagcgtct gcggcggggc ccggccttcc
    28081 tcctcaggga ggatctcgtg gcggcagtgg tccttcgtga aggtgaggcc ggcctccctc
    28141 acctggcgga attctgcaag ccttgccgga ggcggaaaga cagtgaccac gcggctcctg
    28201 ccgggctccg ggcctacccc cgcgggctct ccgcaccagg cattcagcgg cgatgcatcc
    28261 gcccattcct gcattttccg gaagagggtg cgtgaagcca tcacccttcc gtgcatggtg
    28321 taactcctgt ccgagagccg ccctgtggag tctttccgtg gcacagggct gtcggttgcc
    28381 tctccttcaa ggaacccggg ggtgaccctg atcattgcca ccctcgagaa ctcctcagat
    28441 gcctggaggg cggcctccac cgcccggaac gcgctgccgg aacccggctc gatgaccggt
    28501 atctctgcaa gctccccgat gatcctcgtg tcctgtgatg tctgcgagcc cacggcatcg
    28561 gggtcatccc ctgccaccag cagcacaccc ccgacaaggc cctgggcggt tgcctgcagg
    28621 agcgggtcca cgcacgcgtt gactcccacg ttcttcacga tcaccgccgc ccgcctcccc
    28681 aagagagagt cgccgagggc gtattcaagg ccggtcttct cgtttatcac catctctgcc
    28741 ccggtcattg gaccgagttc cgtgacagga tacccgggga ccgtataccg ccggtcagtt
    28801 gcgtcaagga ttgcgtgtgc aagtgcttcg acccctttca tgtatcgtcc ctcccaggca
    28861 ccaggtcgca cccggtgcag gcaacgcaca tcgtcaaggc cgcgcccggg tcgagccccg
    28921 cctcgcacag cgcctctgca tggatcctgt tgatcgagag gatccgctcc tttgaagggc
    28981 gccggtatcc cctgcaccct gccgcaggcg agaggggccg gaccaccggt atcacgccca
    29041 tccgggtgag gtcctgaatg cattcctcca cctgttcgtc cgtctccccg aggccgatga
    29101 taaggttcga gaagacccgg tttttcccaa agagggggac tgactgcgcg agcgcccgcc
    29161 atatgtcgtc ccgggagagg ccccggcaca tcttctcaaa gagtgcgtcg gttgcggtct
    29221 caacgttgaa cttcacctct ctcacaccga gggcgcagag ccgctccgca ctctcgttcg
    29281 tcgggtaaat ggagactccg atggggatgc cgaacttccc aagcttttgc accacgtcga
    29341 ggacatacct ttcctcctcc tccacgctcc ccatcacgcc gcttgtcagg gagatggcat
    29401 cgattcgatc tgcgactccc tctaccatgg ccacgatctc atcgaccgac tttctcctcg
    29461 ggggatgccc tggcacttcg caatacctgc aggagaagat gcacccgctg ctgatcgtga
    29521 tgtatgcctg cctcgggcag tgaagtgcga tcttctcgag gattcccgag atctcttttc
    29581 catccacgga gagcactgcc tcaccgccac ccctgtgcat gacccggatg ctgctttccg
    29641 ggtcaatggc caggcggacg cgccttcccc cgtgggagaa gaataccgaa ccggggcccc
    29701 gtgcaccggg ccccgcggtg gaggcggaga tgtagccgtc cgcaggctct ccctccagtc
    29761 gggcggcccc cgcctcgagg agcctggcct tcagcatgtt ccatcgcaca gctgcagcgc
    29821 ctcctcgaac cgggtggaga acgggatggc ggcgaccgcc ccgatgattc ccctcccctc
    29881 catgagatat tccaggtctc tgcccttcag cgccgcgagg cgctccctgt tcacctcgcc
    29941 gcgcttgact gcccacccat actcaccgag cggagcggga tcgaacccgg tgaacactgc
    30001 catcccggtc ttctcggaga gggtgtagcg ctcgagaagc cgctggaacg atgagaccag
    30061 ccgggccggg tcgcaggtcg cgaactcgca gacgacggcg acgcagttct tggtccggta
    30121 cgggaccggg aagagctgga cgatggtgtg ggagagatac cgggagtctg catcctgtac
    30181 ggcgcttgcg atgttgtggc agagagtcca tgtggcgccc tcctccgggg tgtcagtatc
    30241 gtcaaccccg atcaggacgc gctcgcggcg ggggagccag agagttgccc cggctttctt
    30301 cccaccgcct gaagggtcgc attcggatct cagcaccccc cctgcgaggg agcggcagcc
    30361 cgacgccccc actccgcctc cgccaaggcc tacgtaggag actgcgatct cgtcccggct
    30421 cacctccacc ccgcagatgc cggccgggaa cctcgatccc tcaagtccga ggtccgcctg
    30481 cccgggcgag aggagatacc gggtgctccc ccccaccgtc cggaccgagc gcacgagggg
    30541 gctctttgca tagtgaaggc gtgcccacat ggctccgccg acgcactcga agaactccac
    30601 cagttcgacc tgttttccat cccgggagga gagtgccact atctcaggat acctgatgac
    30661 gtacgggtct ttgaggctct cctgccctgc gttttccggg tctctcatga tccacctgga
    30721 gccacggtgc ctggtgcgga acgcgtgggg caaccgtgga gcgtctctcc cggttgcgcc
    30781 cggttgccct gccatgcttc ctaaacactg gttctcccct catcgccagg actacatgaa
    30841 tcgcaggcga tcccggcagt ttcgccaggg aagacgaaaa gggcggccgc attttgtcac
    30901 cggaattttc gtgaacaaga tatccggcaa caaggtataa aaaaaaatcg atcggattac
    30961 caggagggag aaaaagagag agttcagaga attttatgca gttcgatctt gaatggcccg
    31021 agcgagccac cgaagttacc tgcactgatg aacttcaccc cgggaaccat gactgccgcc
    31081 tttatcccct tggccattgc ctccgagacc gccttctcat ccaggccatc gatgacaatc
    31141 tcgtacaacg cagtaatccc ggcaggcacc ttgctgtcag ggaccttctc acggagcgtg
    31201 ggacagtatt tctcgttggt gcttgccggc atgaacttgt acttgttgga cccaaccttc
    31261 gagccgctcg caacaacccc tcccgggaag ctggtgatga ccccgctcac gctggcgatt
    31321 gcgtctgatg ccgcttgtgc acccatgagg gctgccatct ggttctcgcc catcacaaag
    31381 aaattgccgc cggcaacacc ctttacaatc ccgaactctt cctcgccgac atattcaccc
    31441 tccatgatcg ggatgaccca gcatttcctt cctcccactt ccttctggta ttcatggccg
    31501 tcgccaaaga aatggagctt gaccgggatc ttctcatcag tgccgaccag gccattgaag
    31561 acagccgttg tcggcgaggt gaggacgcac tcggcaaggc gctcgacgac ctgctccttg
    31621 agcttcttct tggatgcaca gatgaggatg gagacgccgg gccttccatc gggggtctcg
    31681 tccggccgga ggaccgattc gatccctgcc tcgcaggggc agccaattgc ggacgtggca
    31741 aagcctgtcg cctcggtcgc tgctttcttt gcccactcct ctgtcaccgc ggtgatgatc
    31801 acgcgggcga tccaggtcgg aaatgcctct gcgtacgtgt cgtcaatggt tactccgttc
    31861 agttgcatgc aactattctc cttatggaga aggggtacca tgatgatggc agaagtaatt
    31921 ttctaaattt tctttggcgg ggaagaggcg gaaccgatca tggcaatttg aacgaagatt
    31981 cttaccaacc aggagcgcag cggggacatc tgcacgatcc tgcataaata tttccatcac
    32041 caatggtaac cagacttata aacattttta tttgactttt gaaattgtcc tctcctgcca
    32101 aaacgcagca ttttacgggg tttttgaggg cgattctatt aatatcagga aatgttaata
    32161 aaaaaaagtt accacaaaac atccgttttt tccctaatgg ccgggaaaat cagacaggtt
    32221 tatgtatata cgttaaccat tgttttttag tcgatttttg aatcgattga aagctggtgc
    32281 gtgaaaaaga atggcatttg cattacacgt gaacatggac cgttgtaccg gttgcaacaa
    32341 ctgtgtggtg gcatgccctg tcgatgcgct cgaactcttc acagtggatc ccgcaacaaa
    32401 cgagaagatc tacaaggtga ggcacggaaa ggccatgatt ctcgatttta agtcagagtt
    32461 atgcgccggt tgtggagtct gcgtcgaagc ctgcccgcat gatgtgattc ggctggcagg
    32521 tcgtg
//
LOCUS       DAOQYR010000017        30526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_1778_length_30526_cov_7.920285, whole genome shotgun sequence.
ACCESSION   DAOQYR010000017 DAOQYR010000000
VERSION     DAOQYR010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 30526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30526
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1778_length_30526_cov_7.920285"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..163)
                     /locus_tag="PKM87_03715"
     CDS             complement(26..163)
                     /locus_tag="PKM87_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29206.1"
                     /translation="MNDQLVVTREAIEAYERRTGFVGIGEFFQRKGLFVLKDGEACNE
                     K"
     gene            complement(302..814)
                     /locus_tag="PKM87_03720"
     CDS             complement(302..814)
                     /locus_tag="PKM87_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29207.1"
                     /translation="MLSPLDIKELFSKMAAHTAACSSSQFLCSTRASFESWFRVELVP
                     VLVHMGIPFQDISPDFTFPDGGKADLCIQSSSEVILFELKPFVSGQDSNKKIEYPRQI
                     NRLLKYLSENSNFLQVITFTTFYGYTEYRMRKFSEDFFSDPRWEIVGPNPLVEGYPLY
                     LVLTSAVRPK"
     gene            1002..2195
                     /gene="yaaA"
                     /locus_tag="PKM87_03725"
     CDS             1002..2195
                     /gene="yaaA"
                     /locus_tag="PKM87_03725"
                     /inference="COORDINATES: protein motif:HMM:NF015820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HNQ29208.1"
                     /translation="MNRLKDPQAIKLLYQVRNFSFDLIFNGGLENKGTLLEQHPLNLG
                     LRRGEDFNGKANARYLPAIDRYTGGFYAGKGSDGKTYELKSSLNHSSHHLLIISGLYG
                     LLLPEEQIQLYESPLEDLQEIQEIWKTENRLTNLLSAYIRAQGINLVVDMTGQRAYQQ
                     LIHWPAIEALKDVRVLHAMAKIGPGEDQIKTFAAALCDPLLRMPASELLALPDSWMLE
                     THHLMLRKTLSPPKGENWPKEPTPIDEFADSLLQFINQMPTSSEESVYSLFVHRNARE
                     LLNEMARKQTEWRLIIHPHVRKDIDSYDNPHIKRLFLEKMQQVLMAYPISRKMKEITE
                     TGRIKEFTIWRIRINDYRLHFCTDETNRFFYIFRFEKKSEDEQTYDYSNLDISTLRRL
                     MLRET"
     gene            complement(2743..3639)
                     /locus_tag="PKM87_03730"
     CDS             complement(2743..3639)
                     /locus_tag="PKM87_03730"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNQ29209.1"
                     /translation="MDIFTHAAAITILLYASGNVALIPFGILGAVIIDIDMAYSVIAR
                     RNTSLFLFFHGGINHSIVGATILSAVMLGITFLLSSAGLILFLFNGQEILPAFAAILS
                     GAYLHLFLDYLAAPGLSVFYPISEKKFGLSLFPMAFYFLFTAISLVMLGFVLLRGLTP
                     ALAVIYSAVFLGIIVLCLGLRWFVSSKFDGKTYSSFTFHPFRRVVIRENPSSWSVTVF
                     EIFRGVIRKETFEKYRNIAQSEVKRYFNVPEIKRHRYFSYISIVEKNDSEITFFDPVR
                     LGDFITYPTWYPSVTVLPDDSL"
     gene            complement(3646..5118)
                     /locus_tag="PKM87_03735"
     CDS             complement(3646..5118)
                     /locus_tag="PKM87_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ29210.1"
                     /translation="MTRTSPENQRYKWTALVVASLGSLLGVLNSTTLIIALPTLMLDL
                     NTTLIGVMWVLISYMLIITILAPASGRLADIYGRKKLYIFGIVAFTIASLLCGVAADI
                     TQLIVFRILQAIGGAVIVANSTLLVVDAFPRHELGRAMGILSVIMAAAFIVGPILGGL
                     LTLIDWRLNFFLNLPIGIAVAYLAHTKLCEVKEFSVKEPFDIVGMLLFAVAFVTLTIY
                     LGIAVLVGPLSVSMLAILAVGIISLAAFIRRELAAPHPLIDLSMFKIRIFAYGQASSF
                     LNAIARGAVMILLILFFQGLRGYDPLMASILIAPMAIGLIFGGPIGGSLSDKYGSRLI
                     STIGLIISLFGLAGLAMMQYDTPYWVLAAWMLVNGLGSGLFQPPNTSAIMASVPFERR
                     GVASSMRAFLGNTGMVISMTIALPLLVATVPLDTMMNMFVMGGMNMPMDTQVTFTNGI
                     AFVFVISSVLTVPAIIVSALRGRDDAGRNMEIVGTSSETP"
     gene            complement(5202..5579)
                     /locus_tag="PKM87_03740"
     CDS             complement(5202..5579)
                     /locus_tag="PKM87_03740"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNQ29211.1"
                     /translation="MLPQPVIPGQVAAFIYLTQDGEIRQDELVSKIGVDKAIGTRVIK
                     KLDEGGYVRRRNDPEDRRVSLISLTNDGAAMKRDIIEVLTSLNRSLLKDFSEEEQETI
                     LRLLDRIIANTDQLFSGISEDSS"
     gene            complement(5985..6962)
                     /locus_tag="PKM87_03745"
     CDS             complement(5985..6962)
                     /locus_tag="PKM87_03745"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ29212.1"
                     /translation="MKSGKNEFGLTFTLSEKVKLEKVSFKNRYGIPVAGHLYVSKDMD
                     TSRKYPALIIGTPYGGVKEQGAGIYAQNMAERGFVALAFDESYNGESGGEPRHVSSPD
                     IFVEDFSAAIDYIGSREFIDRDRIGVIGICGSGAFSITAAQVDPRIKAIATTSMYDMS
                     RMIRKGWADTMTDDQRAQTLVQLGEQRWKDFEHGNPSLPTGFPREPATSIPEGLDPIT
                     SEFFEYYAMERGFHPNAGAAFTMTSSMSFMNFPLMNYIETISPRPILFIIGEHAHSRY
                     FTEDAYKRAAEPKELYIVPGARHIDLYDGGASSYIPFDKLVSFFTENLK"
     gene            complement(6966..7400)
                     /locus_tag="PKM87_03750"
     CDS             complement(6966..7400)
                     /locus_tag="PKM87_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNQ29213.1"
                     /translation="MTKSNVLQERIIFPKGEKIAGEGIANDYFTGTVWLQMLVPPEKI
                     FNCPVYNVTFEPGARNNWHKHPGGQILLVTGGRGYYQEEGKPARLVKAGDVVEILPTV
                     KHWHGAAADSWFVHIGITTNPHAGDAEWLEEVRDEEYAKLGE"
     gene            complement(7533..8075)
                     /locus_tag="PKM87_03755"
     CDS             complement(7533..8075)
                     /locus_tag="PKM87_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HNQ29214.1"
                     /translation="MNTNDSKCLVAYFSRPGNNYVGGTIVNLQVGNTEVVAKMIQELT
                     GGDLFRIEAVHAYPEDYTETIEIAKQEMHTNARPKLTSRLECVDSYDEIFLGYPIWWG
                     TMPMPVFTFLEEHNLSGKTIAPFCTHEGSGLGRSVTDIKKICQQSTVREALAIRGGDV
                     KNAKEAVAGWLREIGVNRDQ"
     gene            8200..8553
                     /locus_tag="PKM87_03760"
     CDS             8200..8553
                     /locus_tag="PKM87_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ29215.1"
                     /translation="MEETPKKYKYSWPIEATLDVIGGKWKPLVMYQLKDGTLRFSEIM
                     DRVHPKITQRMLTKQLRELENDGLIIRKVYPQVPPKVEYSLTEKGRSVMPILDDLCDW
                     GSEHMADEIEFKCED"
     gene            complement(8885..9991)
                     /locus_tag="PKM87_03765"
     CDS             complement(8885..9991)
                     /locus_tag="PKM87_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HNQ29216.1"
                     /translation="MEPYVPQTLPIETIDWIRHVPLIGKANRALARYDGLVQGIVHPE
                     ILLSPLTMREAVLSSRIEGTQASLEEVLEFEAQAKAKYETEKEKDIQEIINYRLAMNS
                     ALELLNERPITINMIRELHRILLTSVRGRDREPGQIRRLQNYIAAPGTPIERAIFVPP
                     APPQLMDALTNWEIYLHADEKDPLVQLAVLKAQFELIHPFRDGNGRIGRMLVPLILYS
                     KGVLSRPIFYISAYLDHHRDEYYARLLGVSRDEDWDGWIAFFLNALVEQAADNTHKAR
                     AILDLYEELKISVPEATRSPYAIQAIDAMFSRPVFKRTDFIAESHIPKQSAYRILQNL
                     QKNGILTEIQEGRGKRAAIYSFPRLLDITEGTIP"
     gene            complement(10267..11316)
                     /locus_tag="PKM87_03770"
     CDS             complement(10267..11316)
                     /locus_tag="PKM87_03770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ29217.1"
                     /translation="MKVAIVGAGIAGSYLGCILEEGGIIPDVYEQGPHDTTCGFRSCG
                     WGAPSAIGAHLADAGLDLDDYYLEPMKSMEFDGLIARTPLCTLDKPALIRDLRARLKI
                     IPHQLTDRESDVYDVVVDATGISRTILPPCTSDLTLPTLQHRVAIEPVAGRAPGPGVY
                     GSQVPGLGYIWIFPLGKSQYHIGAGGLLCDNYAGILNRFLSDLSRGCSITHQCTCPGE
                     IRVASPFFSQPLVAKRRRESGELQRIIGVGESIGTVSPFTGEGILHSMESARILADSW
                     LDEDRYVSRILSRFSWMKKERETLDYLLSDSRDSAPRIRDRWRFYRNARRSGVKLPLQ
                     EAFRRMGSLTRWIEG"
     gene            complement(11698..14169)
                     /locus_tag="PKM87_03775"
     CDS             complement(11698..14169)
                     /locus_tag="PKM87_03775"
                     /inference="COORDINATES: protein motif:HMM:NF041431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEMAR_RS02690 family S-layer glycoprotein"
                     /protein_id="HNQ29218.1"
                     /translation="MLSLALGLIILHSAGAPNVIGPGDQIFIGEEGLDISAALPADVN
                     QIAWFSPGSNIRADAPALLLTIGSRQNFYVSPSQFGHATGTWYAWNQTIGDPAFQVLY
                     PSLNMRVWNQETGMDISGTSIAPGTTVNFRIETNMFPITQRAGYDPATDGYISVLLIS
                     PTGATLTAVADTNGTMIPLQRLDVNTLPWYWVPPGSDAGWDTSAMSETGARLYPTGTY
                     TARLDYNVNRLQDNLQGVPGIGRPIPVHVTLASDRVAITTSGTEIVRGNPFTVTITGV
                     PGADYYLWVRNTGSMTGNPGNQPPLITPNQEGVRMDPPNGPYPIGSYPISTRQGATIR
                     DDVPLSPEGGTRYYALVTLPSSGTRTVQWQTSSATDDGRYLFQAERTVAGRMSSAETS
                     IRVVRGSISIVTGEPRSFSLGEEVVLSGTNTDSDTVYLFMTGPNLPSSGGSLTNPRSA
                     VRTGQPDTFTMVPVDSNNRWEYRWLTGNLGIDAGAYTVFVVSGPNSRNDLGDRRYSTI
                     SISLTRPFVAASIPRTLVARGDPVEIRGTATGNPSPGVAIWIFGVNRFIYSTEAVNPD
                     SSFAYSLREGQTADLSVGQYYVIIQHPGYTNTLDIYPDTARQLVLSTRPVPGSAVFRI
                     GGAGALMSAQAADALIRALASPFIDDTYSAMSFMLVNPQVTITSPGNATIGERITLSG
                     TTNLASGNRLLVEVTSQAFGPTPKDQGSPFSGASGAVTVQAGAGDLNTWSFAFDTASF
                     EPDTYLVRVTGVTVPNAIATRSLLIETFTPTPTPTPPLTTPPTTLTTPEPTTMPPTTA
                     GLTLIPLIVAVAGFVITVGIGRKRI"
     gene            complement(14645..15478)
                     /locus_tag="PKM87_03780"
     CDS             complement(14645..15478)
                     /locus_tag="PKM87_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNQ29219.1"
                     /translation="MHRIIDWNELWKAIHISSPGRTAKDEEPASVWDKKAASYHRNTR
                     EERSATLRDLERLDLQPSDTVLDMGAGTGRLAVPIAARVAHVTALDASGGMLGILRER
                     MVAEGRTNYSCVQKRWEDTLIGQDIWPHEVAIAAFSLGFYDLKAALLKLDAAAKRSVY
                     LFWHAGEWRGPEEMALYRAVFGEYGVLKKGYPDYQFPLNILHDEGIYANVRIYPAIWE
                     ATYESAEEAARHWVTLHYPGLEDLSPVIDFFSRVLRRDTSGRLVHSAIRRTAVIWWTK
                     E"
     gene            15875..16486
                     /locus_tag="PKM87_03785"
     CDS             15875..16486
                     /locus_tag="PKM87_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HNQ29220.1"
                     /translation="MTFSAEQLDALQELTNIGAAHAATALSQMLGCEIGMSVPEINIV
                     DIGKVGTILGEEVSTMVLFQLQGDIPEGGFLILQFSDGSAVRTASMMCGLPDTDRPLG
                     DLDQSALLETGNIMISSFLCASSELLGIMLLPSPPVLVIDIAHAIIESLIAQMRVDAD
                     DVIVFQITLKSEQHGISGNILIFLDEPSLQKISRMLEKILSPS"
     gene            16566..17228
                     /locus_tag="PKM87_03790"
     CDS             16566..17228
                     /locus_tag="PKM87_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HNQ29221.1"
                     /translation="MMIPESFAVELVPWKEAVALSKDLGECIRGKYHPDLVIAIGRGG
                     YVPGRVVCDSLLITTLTSIKIEHWGEAANCYGEAHIRYPLSVDVEGQDLLVVDDVTDT
                     GKTLIAAIEYLQSLRPGSIKTGVLHHKTTSTFIPDFYAKMVPEWHWIVYPWALHEDLV
                     GFVEKVMTGRSATPGKIRSALNKRYHMRPGMDDVVFAARELVERGIAEETRDGYRLKN
                     IL"
     gene            complement(17318..17722)
                     /locus_tag="PKM87_03795"
     CDS             complement(17318..17722)
                     /locus_tag="PKM87_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HNQ29222.1"
                     /translation="MDLQEFLHVHPVKSRLLKLAAGGACEHCGETYPLSLLEMHVIDP
                     RTGAEGDRPDMQKELLILCPECHRFFHARPVQKSVQRELVRYRPKDVKAAMRRILGTR
                     PRTYVPPETDDPEAIFAEMFESGALDLCLNGG"
     gene            complement(17740..18633)
                     /locus_tag="PKM87_03800"
     CDS             complement(17740..18633)
                     /locus_tag="PKM87_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNQ29223.1"
                     /translation="MINERYLKRIQVKPGMKVRLGEYYTGWAQNDALKEAGKEAVKER
                     AMEILEENRAQLASAQELLWASNIYSVLIILQGMDTAGKDGTIKHVMSGVNPQGCKVH
                     SFRVPTDEELDHTFLWRYWRALPNRGEIGIFNRSYYEDVLVVKVHPERLVNLPPGKRG
                     KKFWNARYEDINTFERHLIRNGTVILKFYLHISEDEQKKRLLDRLEDREKLWKFSLSD
                     LEERKFWGTYQEAYEDALTMTSTEEAPWYIIPADHKWVARTLIADIITTRIQSLDLAY
                     PTVSGEAIAQLKDARRQLEEE"
     gene            18894..19868
                     /gene="arcC"
                     /locus_tag="PKM87_03805"
     CDS             18894..19868
                     /gene="arcC"
                     /locus_tag="PKM87_03805"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNQ29224.1"
                     /translation="MRRVRWPPGDERTMRVVIALGGNALLRRGEPMTAEVQRGNVSVA
                     ARLIAPLAGDHELVITHGNGPQVGLLALQAACYRPVEPYPLDVLDAQTEGMIGYMIEQ
                     ELASNLPASQPCATILTMVEVEPDDHAFLHPTKFVGPVYSEDEKEQVSREKGWTFRRD
                     GNAWRRVVPSPLPRRIVEIQPIRWLLSHGAIVICAGGGGIPVIAAPGGRRVLAGVEAV
                     IDKDRASALLAEELKADLLVMATDVEGVFLDWNTPSARLIHSTNPARLRKHHFAAGSM
                     GPKVEAACSFVDATGSRAAIGALSDIRGLVLGDAGTQVSAAGSGETEA"
     gene            19871..21130
                     /locus_tag="PKM87_03810"
     CDS             19871..21130
                     /locus_tag="PKM87_03810"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF002381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HNQ29225.1"
                     /translation="MSEFGVYSEVGRLRKVLVHRPDLSARRLTPGNHQDLLFDDVLWV
                     ERAMEEHDAFVRVLEEEGVRVYRLASLLADVLAGKEARRWVIERAVHPMTVGRSACGS
                     VRACLMEMEPGALAEHLIGGLTRSELECIYLEGMTRSSLQVAASDPDSFILPPLPNTL
                     YTRDTSSWICGGFTLNPMFWPARRAEVVNTAAIYRFHPLFSNGGIRTWYSCLDHGNAA
                     PPASSATASMEGGDIMPIGRDTLLVGMGERTGGPMVEELARVLLMSGAVERIIVAQMS
                     HDRAHMHLDTVCTMLDESTVTVFPKVVEKIRAFSIRPGEGERIFEVTREEDFLGAVAD
                     ALGVGRLLVIPTGGDEYQAAREQWDDGNNVLAIAPGRVVAYDRNTCTNRNFEKAGIDV
                     IEIAGSELSRGRGGGHCLTCPLKRDPI"
     gene            21207..21956
                     /locus_tag="PKM87_03815"
     CDS             21207..21956
                     /locus_tag="PKM87_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNQ29226.1"
                     /translation="MTPVLLPLFQKRIFDRRQELEAVREYPVEKLADIIGEVGFSCTY
                     CARCCTRAFNGHVLLLDEDAARIRSIDPTALEPQPEYDLCDQYGTFYVSGYTLRAMPG
                     ESGECHFLTDGRCRIYPDRPLICRVYPYMLHREPDEVGVVDWRQVSGLDQHGDYHCTV
                     PRDIAREIACEVKAFECAVISHEIAFLLYAGSYFSVHGLRHVRKRFDDGMRSLRTGTA
                     VRVKVFFGGTFESWVVRGGKCTLSDEKRQGV"
     gene            complement(21957..22373)
                     /locus_tag="PKM87_03820"
     CDS             complement(21957..22373)
                     /locus_tag="PKM87_03820"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HNQ29227.1"
                     /translation="MGLFDKLKGSKAVELNPKSALVLSAITVIASDGVIDEAEMMDLA
                     KIARGDRNAVDTAVKILQGITLPEAVDLVAKALNDQQRIATMAILTDLAMSDGVLAGN
                     EQKILQLYMEKFGITETALKPIIDAIAIKNDFSVFS"
     gene            complement(22468..23493)
                     /locus_tag="PKM87_03825"
     CDS             complement(22468..23493)
                     /locus_tag="PKM87_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNQ29228.1"
                     /translation="MYTGIQLFRPYYILIVLACGVILLAGMKAASPILGPVLCAGFIS
                     ILFIVLKHWLEKKGFSPRLAVVAVILLYFTTLAGFVLAVGVSLLQLASQLPLYQEGLE
                     SLLPSATECGQVSFADLLTLLPSDAIDLLMTTGRGVIALLGDLLTAALIVVFTPLFIL
                     AESAGLTRKLQGVIVHLSGRMESAERFGRQMVEYLVVRTEVNLANGVVNGLFFACIGV
                     DFAILWGFLAFVLGYVVYVGFWLAVIPPMVLAWLEMGPAAALLVLIGAGIINTVAEYL
                     LFPHLAGYQLNLSPLVIFLSIFFWWLILGAFGVLLAVPLTMAVQAILETWDETRWLGE
                     LLGNESP"
     gene            23651..24214
                     /locus_tag="PKM87_03830"
     CDS             23651..24214
                     /locus_tag="PKM87_03830"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HNQ29229.1"
                     /translation="MTDEVTSAALKVDDVMHLFPWWIVLLQGIVSLLLGIMFLAYPMG
                     TLFVMVTFLGAYWFVSGIFALVSLATDKTNMGVKVVLALLGIIAGILILAYPYYSTII
                     VPEIFVIMIAVWGVMMGCVSLFSSFKGGGIGAAVIGVLAIIFGCIILFNPLITVIYLP
                     FVLGIFGIIGGLAAIFFSFQVKSAAGA"
     gene            24402..24863
                     /locus_tag="PKM87_03835"
     CDS             24402..24863
                     /locus_tag="PKM87_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29230.1"
                     /translation="MKKLIGLFTLCMVAFMVAGCCMPPPTTEGIQTISPTSTAPEPSI
                     GHNMIPQPTDVIPPFYSITVQVGKNTVSVDPYITVSFRGGPGQEFTQSMHAIVYRSDG
                     STESGTVAYPQVGSEIILSGTTGTDRVVVDVTLVTGETYHAYDALVPFRNL"
     gene            24979..26322
                     /locus_tag="PKM87_03840"
     CDS             24979..26322
                     /locus_tag="PKM87_03840"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNQ29231.1"
                     /translation="MIEGDCVNGFKACVLVLGIVMCLAAGASGAEPLPGTTAAPSTLP
                     GADQGFFLIDSLPRDADVYFDKNFLGETPVTVAVSITGKPAHEIDIIRGGYEPWSSVY
                     EGNPRPGQTVTVMATLIHATASGAIQVTSSPSGAISTMDRAQTMETPCTYATVPIGDH
                     EVSVYLPGYQTYYTSVNVQKGETAHVSAELSLVRAGGAISVSSTPAQAAVYVDGIYRG
                     VTPTTAGNLAPGQHSVKISRSGYHDWTGQVEILAGIESAISPTLVRDPEPASGTVSIT
                     SSPPGADVYADGVYVGQTRTGSPLVYREGQPGLHELHLAMTGYQDYTATGVVRAGENY
                     DIVISLAPDPQASTGKISLVSMPSGADVFLNNAHRGPTPITLHSLEPGSYTLLVRLSG
                     YRDWQSDVRVSAGETVEIKATLIQKPPSDRPQSGIPAYLTLGSLTIAALLWFSRK"
     gene            complement(26384..29068)
                     /locus_tag="PKM87_03845"
     CDS             complement(26384..29068)
                     /locus_tag="PKM87_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ29232.1"
                     /translation="MNHLSEERLSDIRDLLASHPQGLSINEISARLHRHRNSVSRDLH
                     ALSLSGQVVQHAFGTTRVYSLSRRVPITSILHYIHDMVLVLDNNGVILSANAPLLAFL
                     SCTSEALKGVDIKSKDHPLLAAICAQLVEDSQKEVVSTHAFRICRGDEPFFLKTKMVP
                     IVFENGKSGVVCILTDITNEIRSKEALKTSKLQYMALVDAMPEMVAQFLPDGTIISAN
                     DVFSSFFSLSLKGDSYGSLFSLIYPEDTNAFLSHLATLSPEQPTATVSFRVSPKGGLP
                     VWHTWVIQGLYDKDSMPLVYQGIGHDITEELVNRNLDVARTRELAFVASKSREFMAMD
                     PVADMYECIASHITEMIPGSIAAVFEIDSGIWTCTLKATAPRNSSGRGFLPGPIDPDV
                     HTCSLAGKDGSLVNFREALLSGSLVHPGGFLLQRIFPQGYIAVDTLPDQERRDTDACS
                     AGLVWEGSLYGAIEVILPQSEWHNYSRCLDTYLGVASLALQKRMFEHALRTSEERFKR
                     IATTNPHPISIIDSSGRYIYLSPRFTELFGYTQEDIPTGREWFMQAFPDLDEQKRARE
                     TWKMDLAESRPGEIRPRQFRVRCKDGLFKEISFLPVGMSNGQQLIVYEDVTPRLEAVQ
                     TRNLLFDIFRSSHDGIFSATIDGRILSWNPAAERIYGYSAEEVEGGDVCILEPPSLKG
                     EIPAILERVRRGEHIVNFETQRIKKDGKLIDVSLTVSPIYSKGRTIMGASTIIRDITA
                     RKSEERLRRAEMQYRELVDNINVGVYRSTGDPEGRFVWGNTSLLRILGYDTLDDLQDI
                     PVSDMFMHAGGRADLLAELMQQGFVKNREIILKKADGSPIHVLVTALATFAPEGSISH
                     INGIVEDITSQRVLEQKVARLSDFHDEVHLPVPPPPRD"
     gene            29329..29709
                     /locus_tag="PKM87_03850"
     CDS             29329..29709
                     /locus_tag="PKM87_03850"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ29233.1"
                     /translation="MGGTLCMDHITQHTGKSVLNISHQADYSFRVGTESAHRGEYLRA
                     LEHFEKALSSDPHFAMAWHEKGNCLDELGRCDEALSSYDTAIQLDPHHAEAWFNKGLT
                     LKKMGREKEAYSCMNHGVDLALGR"
     gene            30244..>30526
                     /locus_tag="PKM87_03855"
     CDS             30244..>30526
                     /locus_tag="PKM87_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNQ29234.1"
                     /translation="MNAKYVQTTCPYCGTGCSFNLVVMDGKVTGTAPYQRSPVNEGKV
                     CPKGTYAHEFINAPDRLTKPLIKKDGKFVEASWDEAYDLIAKKFKQYKPD"
BASE COUNT         7237 a         7855 c         8238 g         7196 t
ORIGIN      
        1 gatacgtcag gtctggagga gcccactact tctcgttgca tgcttcaccg tccttgagga
       61 caaagagccc tttcctctga aagaactccc cgatgcccac gaaccctgtc cggcgttcgt
      121 aggcctcgat tgcttcccgt gtgactacga gttgatcgtt catgtgtcat tcctgcaggg
      181 aaacgatgga agtcatttcc tgcagactag catggcaaaa tgggtgaaat aaggataaac
      241 gggggcttct gaaagtgggg aaggaaaagc gttactgtat cggataaaga aatcaaatta
      301 tttatttcgg ccttacagca cttgtcagaa caagataaag gggatatcct tcgaccaaag
      361 ggttggggcc aacaatttcc caacgaggat ctgagaaaaa gtcttcggaa aattttctca
      421 ttctgtattc agtatagcca taaaatgttg tgaaagtgat aacctgcaaa aagttagaat
      481 tttcactaag atattttagt agtcgattga tttgacgagg atactcgatt tttttatttg
      541 aatcttgccc tgaaacaaaa ggtttcaatt cgaaaagaat tacttcactg ctcgattgta
      601 tacaaagatc ggctttacct ccatccggaa aagtgaaatc aggagagata tcctgaaaag
      661 gtattcccat atgtactaaa actggaacca gttctactct gaaccatgat tcaaaggatg
      721 ctcgggttga acaaagaaat tgagacgatg agcaggccgc agtatgagcg gccatttttg
      781 aaaagagttc cttgatatca agcggcgata gcataattcc tccttatttc catctccttt
      841 atctcatctc ctttatatat ccaatttgag tagtcatgtc ctcgaatatt aattcggtaa
      901 atattcatca cccaaaaagt ccagttttgt ttcttgggac ctgttcgttg aataaatcga
      961 tcatttccaa caatgagtca tatcagaagg atctatctat tgtgaaccgt ctcaaagatc
     1021 cacaagcgat taaactactc tatcaagtga gaaatttttc ttttgaccta attttcaatg
     1081 ggggccttga gaataaggga actttactcg aacagcatcc actgaatctt ggattgcgtc
     1141 gaggagaaga ctttaatgga aaggcaaatg cgagatatct gcccgcgatt gatcgctata
     1201 ctggaggatt ttatgcggga aaaggcagtg atggtaaaac ctacgagctt aagtccagtc
     1261 ttaatcactc ttctcaccac ctgctcatta tttctggact ttatgggcta cttcttccag
     1321 aagaacagat tcaattatac gaatcacccc tcgaagatct ccaagagata caggaaattt
     1381 ggaaaacaga aaataggcta accaatctcc tctccgcata tattagggca caaggaataa
     1441 atttggtcgt tgatatgacg gggcaacgag catatcaaca actaattcac tggcctgcaa
     1501 ttgaagcgtt aaaagatgta cgggttctcc atgcaatggc aaagattggt cctggcgagg
     1561 atcaaattaa gacatttgca gcagcgctat gtgatccact tctccgaatg cctgcgtcag
     1621 agctgctcgc acttcctgat tcgtggatgc ttgaaactca tcacctgatg cttcggaaga
     1681 cgttatcacc gccaaaggga gagaattggc cgaaagaacc caccccaatt gatgaatttg
     1741 cagattcctt gcttcagttt attaaccaaa tgccgaccag cagcgaggaa tcagtttatt
     1801 cattgtttgt tcatcgaaat gcaagggaat tactcaacga aatggcacga aaacaaacag
     1861 aatggagatt aatcattcat ccccatgtgc ggaaagatat tgatagttat gataatccgc
     1921 atataaagcg tctattcctt gaaaaaatgc aacaggttct catggcttat ccgatatcgc
     1981 gtaaaatgaa agaaataact gaaacaggac gaattaagga gttcacaatt tggagaataa
     2041 gaataaatga ttatcgtctt catttttgca cagatgagac taatcgattt ttctatatat
     2101 tcagatttga aaagaagagc gaagacgaac agacctacga ttattcgaac ctggatatct
     2161 caacattaag gcgtttaatg ttacgtgaga cttgattttt ttatttatct caatattctt
     2221 ccaaggaaat tgtgattctg cgcagatagt tgaaagagag atatgcggca ggtccgggaa
     2281 tcatcagatc cagatactta tactttgcat cttctctttg cgctggtacg aatcggagcg
     2341 attttcacgc tccttttgga cgccgggctg aggcactttt cgacaaatag ccataaatta
     2401 cacttatttt tagatttaga gcctaattgt aaggaattta atgacttaat ataagaggtc
     2461 aaaatttttc cggactccca aacactgaaa agacccatcc ctccaagggc gaggagtgcg
     2521 ggaactagcg actgcatcag cggagcggaa aaaaattctt caaaaaagaa aatggagaat
     2581 ttacggcggt tggccgtatg cgattataaa aaggcggcaa aggattgtcc cttatgccga
     2641 tcctcgatca gctctgcctg aggtgatgtg aacacatgaa ggatgacatt gaatttctct
     2701 gcgacaggga gttgacaccc aaagatccac caatccatac ctttaaagtg aatcatcagg
     2761 gagtactgta acagagggat accaggtggg ataggtgatg aaatcaccca gccgcaccgg
     2821 gtcaaagaag gttatctcgc tgtcgttctt ttctacaatg ctgatatagg aaaagtacct
     2881 gtgccgcttt atttccggga cattgaagta tctttttact tcggactgtg caatattacg
     2941 atatttctca aatgtctctt tccggataac acccctgaaa atctcaaaaa ccgtaacaga
     3001 ccaggacgaa gggttttcgc gtataacaac cctccggaaa ggatgaaatg taaacgagga
     3061 atacgttttc ccgtcgaatt tactggatac aaaccatctc aatcccaagc agagaacgat
     3121 gatccccaga aaaaccgcac tgtaaatcac tgcaagtgca ggcgtaagac ctctcaggag
     3181 aacaaatccc agcataacaa gcgaaatagc ggtaaacagg aaatagaatg ccatagggaa
     3241 caaactcaac ccaaactttt tttcactaat ggggtagaag acagagagtc cgggtgctgc
     3301 cagatagtcc aggaacaggt gaaggtatgc accggagagt attgccgcaa acgccggaag
     3361 aatctcctgt ccattaaaaa ggaatagaat aagtcctgca gaggacagga gaaatgttat
     3421 acccaacatt acagccgaaa gaatggtggc tccgacgata ctatggttga tcccaccgtg
     3481 aaagaagaga aatagagagg tatttctgcg ggcaataacg gagtatgcca tgtcaatgtc
     3541 aataattact gctccaagta tcccaaacgg aataagtgcg acattgcctg atgcatagag
     3601 cagtattgta atggctgctg catgagtgaa aatatccata attaatcagg gggtctctga
     3661 agatgtgccg actatctcca tgtttcttcc cgcatcatct ctgccccgca gtgctgaaac
     3721 aataattgcc gggaccgtga gcaccgatga gatcacgaaa acaaacgcaa tcccattagt
     3781 aaacgtgacc tgtgtatcca tcggcatatt catgccgccc atgacaaaca tgttcatcat
     3841 cgtatcgagt ggaacggttg cgaccagcag agggagggca atggtcatcg agattaccat
     3901 gcccgtgttt ccgaggaatg cacgcatcga tgatgccact ccccggcgtt cgaagggaac
     3961 ggacgccatg atcgcactgg tattcggggg ctggaaaagc ccgcttccta atccgtttac
     4021 cagcatccag gctgcaagaa cccagtacgg cgtatcgtat tgcatcatgg caagcccggc
     4081 cagcccgaag agagagatga tgagtccgat ggtgctgatc agccgggacc catatttatc
     4141 ggaaagggat ccgcctattg gcccgccgaa gataagcccg atcgccattg gtgcaatgag
     4201 gatacttgcc ataagcggat cataccctct cagaccctgg aagaagagga tcaacagaat
     4261 catcacggca ccgcgggcaa ttgcgttcaa aaacgagctt gcctgcccgt aggcaaagat
     4321 gcgaattttg aacatggaca gatcgatgag cggatgtggg gcggcaagct cgcgccggat
     4381 aaatgcggca agcgaaatga tcccgaccgc gagaattgcc agcatcgaaa ccgacagagg
     4441 acccacgaga acggcgatgc ccagatagat ggtcagcgtc acgaatgcga ccgcaaagag
     4501 aagcatcccc actatatcaa acggctcctt cacgctgaat tctttcactt cgcaaagctt
     4561 ggtatgggcg agatatgcaa cagcgattcc gatgggcaga ttgaggaaaa agttcagccg
     4621 ccagtcgatc aatgtcagaa ggccgccaag gattgggccg acaataaaag cggctgccat
     4681 tataacggaa aggattccca tggccctccc gagttcatga cggggaaatg catcgacaac
     4741 gaggagcgtg ctgttcgcaa caataactgc gccgccgata gcctggagga tgcggaatac
     4801 aatgagttgg gtgatatctg ccgctactcc gcagaggaga gatgcgatgg tgaaagcgac
     4861 aatcccgaag atatagagct tttttcgtcc gtaaatgtca gccaaacgtc cgcttgctgg
     4921 tgcaaggatg gtgatgatca gcatatatga gatcagtacc cacatgaccc caatgagggt
     4981 ggtattgagg tccaacatta atgtcggaag tgcaatgatc agggtggtag agttcagaac
     5041 cccaagaagg gaacccagcg atgcaactac aagtgctgtc catttatagc gttggttttc
     5101 aggagatgtt cgagtcaaac gaatatcctt ccttttggtt ttattgaggc attcatggta
     5161 agggaaaaga tgcgaacgca ggatctcttt ttcactattt ttcaggagga gtcttctgat
     5221 atgccggaga ataactggtc tgtgtttgcg attattctgt ccaggagcct gagaatcgtc
     5281 tcctgctcct cctcggaaaa atccttcaaa agggatctgt taagagaagt gagaacctcg
     5341 ataatatccc gtttcatcgc ggcaccatca ttcgtgaggc tgatgaggga gacccggcga
     5401 tcttccgggt cattacgccg tcggacatat ccgccctcat cgagtttttt tatcacccgt
     5461 gtgccgattg ctttatctac gccgattttc gagaccagtt catcctgtcg tatttcacca
     5521 tcctgcgtga gataaatgaa ggctgccacc tggcccggaa tgaccggctg aggcagcatt
     5581 tttttcagaa ttttttgatt ggaccagtaa aggattccaa tctttttcgt tatgcgttcg
     5641 gtttccatgt gcttttagtt gatatgtcaa ccgattatat aagattatgc aaaaggccgg
     5701 atcccccgcc gctctcaacg gcctcatcat acgaaatctc aaatttcact tcatcctctc
     5761 aaaatggtaa acaggtgtcc catccaggga gaggatggtg atgcacaaag tcaatttaaa
     5821 agttctctca tggagcattg agcgaaaata atgaatctgt ccgacggagc gtgaaatccc
     5881 ctcaacacgt taaggaaaca agtcacctcc ggtaatttcc cggttcatct ttatcacaag
     5941 atattttcag atctatcttt ttttccttca cccttcactt aagttcactt cagattttct
     6001 gtgaagaaag ataccaattt atcgaaggga atataactac tagctcctcc atcgtaaaga
     6061 tcgatatgcc gtgctccggg aacaatgtag agttccttgg gttctgcagc ccttttgtag
     6121 gcatcttcgg tgaaatacct ggagtgagca tgctcgccga tgatgaacaa tataggtctt
     6181 ggtgagatcg tttcgatgta gttcatcaaa ggaaagttca tgaatgacat gctgctggtc
     6241 atggtgaatg ctgctccggc attgggatgg aatccacgct ccattgcata atattcaaag
     6301 aactcacttg taatggggtc caatccttcc ggaattgaag tcgctggttc acgagggaat
     6361 ccggtaggca acgatgggtt gccatgttca aagtccttcc atctttgctc gccaagctga
     6421 accaatgtct gggcacgctg atcatcagtc atcgtgtctg cccatccctt tcgtatcatc
     6481 ctgctcatgt catacatgct tgtagtggca atcgccttga tacgagggtc gacctgggcc
     6541 gcagtgattg aaaatgctcc gcttccacag atgccgataa ctccaatcct atcccggtct
     6601 ataaactccc gggacccgat atagtctatc gccgcactga aatcttcgac aaaaatgtcc
     6661 ggagaagaca cgtgccgtgg ttccccgccg ctctctccgt tatacgattc gtcaaacgca
     6721 agggctacaa aacctctctc tgccatattc tgcgcataga taccggcacc ctgctctttt
     6781 acacctccat acggtgttcc tataatgagt gcaggatact tcctggacgt atccatgtct
     6841 tttgacacat acaagtgtcc tgcaacaggt atcccgtacc tgttcttgaa cgagaccttc
     6901 tccagcttta ccttttcact caatgtgaat gttaacccaa attcattttt tccagatttc
     6961 attttttatt cccccagttt agcatactct tcatcacgta cttcctcaag ccattctgca
     7021 tctccagcat gtggattcgt agtgattcca atatgcacaa accaactatc tgctgctgcc
     7081 ccatgccagt gcttgacagt tggaaggatt tctacaacat ctccagcttt caccaatctg
     7141 gcaggtttgc cttcctcctg ataatatccc ctgccaccgg tcacgagcag gatctgtccg
     7201 ccgggatgct tgtgccagtt gttccgtgca cccggttcaa atgtgacatt gtataccggg
     7261 cagttgaaga tcttttccgg cgggaccagc atctggagcc aaaccgtccc ggtgaaataa
     7321 tcgtttgcaa ttccttcacc ggcaatcttt tccccttttg ggaaaataat ccgttcttgt
     7381 aaaacgtttg atttcgtcat aagacatcat ctcctgattt ttggtaagag tgacatttgg
     7441 ttatctatac agccgaagct actcgcagag acttccaatc cgttccttcc taatgttcgt
     7501 gtgtgcatag gttgacctcc tttagaacat gatcactgat cccggttcac cccgatctcc
     7561 cgcagccacc cggccacggc ttccttcgcg tttttcacgt caccgccccg gattgcaagg
     7621 gcctcacgaa cggttgactg ctggcatatc ttcttgatat cagtcacact ccgcccaagg
     7681 ccgctccctt cgtgcgtgca gaacggggcg atggtcttcc cggagagatt gtgttcctca
     7741 aggaaggtga agaccggcat cggcatcgtt ccccaccaga taggatagcc gagaaagatc
     7801 tcatcataag aatccacgca ttccaaccgg ctggtaagct tcggtctggc attcgtgtgc
     7861 atttcctgct tagctatttc aatggtttca gtatagtcct cgggatacgc atgcactgct
     7921 tcaatacgaa acagatcgcc tccagtcaat tcctggatca tcttggctac aacttccgtg
     7981 ttgccgacct gcaggtttac gatagttccg ccgacatagt tgtttcccgg gcgcgagaag
     8041 tatgcgacca ggcattttga atcatttgtg ttcattttca tctcttccac ctatgacccc
     8101 cgggcggtat cgctcgtgtt cctgtagtat atattaagta tgtatgacat atatagtata
     8161 aacaaacata tatgtgatat acttacagga ttgatacttt tggaagaaac tccgaaaaaa
     8221 tacaaataca gctggccgat cgaggctaca ctggacgtaa tcggcggcaa atggaaaccg
     8281 ctcgtaatgt accagctcaa ggacgggaca cttcggttca gcgagattat ggaccgggtg
     8341 caccccaaga tcacgcagcg gatgcttaca aaacagctcc gcgaactgga gaacgacgga
     8401 ctcatcatac ggaaggtgta tccccaggtt ccgcccaagg tggagtattc gctcacggaa
     8461 aagggccggt cggtgatgcc gattctcgat gatctctgtg attggggatc cgagcacatg
     8521 gcggacgaga tcgaatttaa gtgcgaggat taactaaaag aaatcgacga attactcatt
     8581 acactggatg aggcattgaa ccttctttgc acaaactccg ggctgaggca tgattggtat
     8641 tttaccaaca attttcggtt ataatcaaat ttaaagccta tatgttgcgt atttttaata
     8701 ttttttccga ttggcagatc aatctggtta ctaagtgcac tctgaaatag aagacttccc
     8761 ttcgacccag atgagattgg gcttttgaca tgttctctcg ttgtgtaaca cattgcatga
     8821 tatggtgtta cacaagcggt ttaatgtgct acaagtgtta cacaagggga tcctggcacg
     8881 tggattatgg aatcgttcct tccgtgatat cgagtaaacg cgggaagcta tagatggctg
     8941 cacgtttccc gcgtccctcc tgaatctcag tgaggattcc attcttctgc aggttttgaa
     9001 ggatccggta agcgctctgt ttcgggatgt gggattctgc aatgaaatca gtcctcttga
     9061 agacagggcg ggaaaacatc gcgtcgattg cctggattgc atagggagag cgggtagcct
     9121 ctgggacact gatcttcagt tcctcgtaga ggtcgaggat ggccctggct ttatgggtat
     9181 tgtcggcagc ctgttcgacg agcgcattga ggaaaaatgc aatccatccg tcccagtcct
     9241 cgtcgcgaga tactccaagg aggcgtgcat aatactcatc ccggtggtga tcgagatacg
     9301 cactgatata gaatatcggc cgggacagga cccccttgct atacagtatc agcggaacca
     9361 gcatccgccc gatcctcccg ttaccatcgc ggaacggatg gataagctcg aactgggcct
     9421 tgaggacggc gagctggaca agcgggtctt tctcatctgc atggagatag atctcccagt
     9481 tggtgagtgc atccatcagt tgcggaggag cgggtgggac aaagatcgcc ctttcgatgg
     9541 gtgtccctgg agcggcgata taattctgca ggcgcctgat ctgcccgggc tcccggtccc
     9601 gcccgcggac actcgtcagg aggatccggt gaagctcgcg gatcatgttg atggtgatag
     9661 gtcgctcatt gagaagttca agggcggaat tcattgcgag ccggtaattg ataatctcct
     9721 ggatatcctt ctccttctcg gtctcgtatt tcgccttggc ctgggcctcg aactccagga
     9781 cttcctcgag ggaagcctgg gtgccctcga tacgtgaaga cagtaccgct tcccgcatcg
     9841 tcaatgggga gaggaggatt tcgggatgca cgatgccctg gacgagtccg tcataccgtg
     9901 ccagcgcacg gttcgctttc cctatgagcg ggacgtgccg tatccagtct atggtctcga
     9961 tcggcagggt ctgtgggacg tagggctcca ttgttgatga ccgggctctc ttcctatatt
    10021 catgatcttg ttctggaggt aaattagttg gtggaaaaat ttgatcgaac tcactccgga
    10081 taatcgttgt tctttaaaga aatatacagg gcggcaggtc cgggaatcac cagatgcgga
    10141 tacttatact ttgcaacttc agttcgcgcg agtgtaaatc ggagcgattt tcaggcttct
    10201 tttggacgcc gggctgaggc atcaagtttc actggtatcg cgtttatccg tttttttccg
    10261 ctctatctac ccctcgatcc accgtgtgag gctccccatc cgccggaacg cctcctgcag
    10321 cggtagtttg actcctgatc tccgtgcatt ccggtagaag cgccagcggt ccctgattct
    10381 tggggctgaa tccctgctat ctgaaaggag ataatccagg gtctcccgct cctttttcat
    10441 ccaggaaaac cgtgagagta tacgggatac gtatctgtct tcgtcgagcc atgaatcagc
    10501 aaggatcctt gcagattcca tggagtggag aatcccttct ccggtaaatg gtgatacggt
    10561 gccaatcgat tctccgacac caatgatccg ctgcaattct ccgctctccc gtctcctttt
    10621 cgcaaccagt ggctgtgaga aaaagggtga tgccacccgg atctcgccgg gacaggtgca
    10681 ttggtgggtg atggaacagc ctcttgaaag gtcgctgaga aaccggttca ggatccctgc
    10741 ataattgtca cataagagtc ccccggcacc gatgtgatac tggctttttc cgagtgggaa
    10801 gatccagata tagccaagcc cggggacctg tgaaccatag actcccggcc ccggcgccct
    10861 gccggccacc ggctcgatag ccaccctgtg ctgaagcgtt ggcagggtga ggtcggaggt
    10921 gcaggggggg aggatggtcc gggatatccc tgttgcatcc accaccacgt cgtacacgtc
    10981 gctctcgcga tccgtcaact gatgcggaat tatcttcaga cgtgccctga ggtccctgat
    11041 gagcgcgggc ttatcgagcg tgcatagcgg ggttctggca ataagaccgt cgaattccat
    11101 tgatttcatc ggttccaggt aataatcgtc aagatcaagc cctgcatcgg cgagatgcgc
    11161 acctattgcc gaaggtgcac cccacccgca ggagcgaaaa ccgcacgtgg tgtcatgggg
    11221 cccctgctcg tagacatccg gaattatccc accttcttcg aggatacagc caaggtagct
    11281 cccggcaata cccgccccga caattgcaac cttcacagta tccctcaaaa ggtcagcatc
    11341 aacatctatt gcgtgatggc gtgtacaagg aatgtgtcct ctttgcggga gtatcaatct
    11401 gctggcaccc atcgtgaaat cctcaggatt ttgtggtaac atgaaagatc cctgtttttt
    11461 gttcgcaaca atccaggcaa ggcatggatc agcgatagca ggcctcaatg gaaccgcatt
    11521 ttgctggaac ccagttcgga gtgacgagca agacactcac atgcaatcgc aagaaatgtc
    11581 cggatgattg atattggaat ccaccggttt ttttcatacg aataatcccg ctgtaattca
    11641 gagtatctct ggatgggggc aggcggatgc aggtggcacg gattgcaaaa aaatggttca
    11701 gatcctcttt cttccaattc caaccgttat cacgaagccg gcaacggcta cgatgagggg
    11761 aatgagggtt aggcctgcag tggtgggggg catggtggtg ggctcagggg ttgtcagggt
    11821 cgtagggggg gtagtgagtg gtggtgtggg cgtgggggtt ggagtgaagg tctcgatgag
    11881 gagagacctt gttgcaatgg cattggggac cgtcactccg gtcacccgga cgaggtacgt
    11941 gtcaggttca aacgatgcgg tatcgaaggc aaatgaccat gtgttcaggt ctcccgcccc
    12001 cgcctgcacc gtgacagccc cggaagcacc ggaaaacgga ctgccctggt cctttggagt
    12061 gggaccgaat gcctgtgacg tcacctccac aagcaaccgg ttgcctgacg caaggttggt
    12121 ggtgcccgag agggttatcc tctccccgat ggtggcattc ccgggtgagg ttatcgtcac
    12181 ctgcggattc acaagcatga acgacatggc gctgtaggta tcgtcaatga acgggctcgc
    12241 aagtgccctg atcagggcat cggcagcctg cgcgctcatc aacgcacccg ctccccctat
    12301 cctgaagacg gcgcttccag ggacagggcg cgtggagagg acaagctgac gggcggtgtc
    12361 cggatagata tcaagggtgt tggtatatcc cgggtgctgg atgataacat aatactgccc
    12421 gacagacagg tccgcggtct gtccctcgcg tagactgtag gcaaacgaac tgtcgggatt
    12481 gaccgcctcg gtcgagtaga tgaacctgtt cacgccgaag atccagatcg ccacgcctgg
    12541 agaggggttc ccggtcgcgg tgccccggat ctccaccggg tcaccccggg ccacaagggt
    12601 ccggggaata ctcgcagcga caaatggcct tgtaagggat atggatatgg tcgaataacg
    12661 acgatctccc aggtcgttcc tcgaattcgg tccggaaacc acgaagacgg tataagcccc
    12721 ggcatcgatg ccgagattcc cggtaagcca ccggtactcc cacctgttgt tggagtcgac
    12781 cggcaccatc gtgaacgtat caggctgccc ggttctcacg gcactgcgcg ggttggtaag
    12841 actgcctccg gaagagggta ggtttgggcc ggtcatgaag agatacacag tatccgaatc
    12901 agtattcgtc cccgagagga ctacctcctc cccaagggag aaggacctgg gttcgccggt
    12961 tacaatactg atcgatcccc tgacgacccg gatgctggtt tccgcggatg acatccgccc
    13021 ggcaacggtg cgctctgcct ggaataaata ccgtccgtca tccgtggcag aggacgtctg
    13081 ccactggacg gtacgggtcc cgcttgatgg gagggtcacg agcgcataat agcgtgtccc
    13141 gccttcaggc gagagaggca cgtcatcccg aattgttgct ccctgtctcg ttgatatggg
    13201 atacgatccg atggggtaag ggccgttggg agggtccatc cgtacccctt cctggttcgg
    13261 agtgataaga ggcggctggt tgccgggatt gccggtcata ctcccggtat tcctgaccca
    13321 gagatagtag tctgcaccag gcacccctgt gatagtgaca gtgaacgggt ttccacgcac
    13381 aatttcggta ccactcgtcg tgatagcaac ccggtcagat gcgagggtga catggacagg
    13441 gattgggcgg ccgattccgg gaacaccctg gagattgtcc tgcaacctgt tcacattgta
    13501 atccaggcgt gcagtatacg tcccggtggg gtagaggcgg gcccctgtct cgctcatggc
    13561 agaggtgtcc cacccggcat cggagcctgg tgggacccag taccagggga gagtgttgac
    13621 atcaaggcgc tggagaggaa tcatagtgcc gtttgtatca gcgacagcgg tcagtgtcgc
    13681 ccccgtgggg gatatgagga gcaccgagat atacccgtct gttgcgggat cgtagccggc
    13741 ccgttgtgtg atcgggaaca tgttggtctc gatcctgaag ttcacagtgg ttcctggcgc
    13801 aatgcttgtg ccggatatat ccatcccggt ctcctggttc catactctca tattcagcga
    13861 aggatacagc acctggaatg ctgggtcgcc gatagtctgg ttccatgcgt accaggttcc
    13921 cgttgcgtgc ccgaactggc ttggtgaaac atagaagttt tgcctgctcc cgatcgtgag
    13981 caacaatgcc ggtgcatcag cccggatgtt cgaccctggg gagaaccagg caatctggtt
    14041 cacgtctgct ggtaaggcag cagagatatc cagtccttct tccccgataa atatctggtc
    14101 tccgggcccg atgacatttg gtgctcctgc ggagtgaaga ataataaggc cgagtgccag
    14161 gctgagcatc aaagcagatg atagtctcat tggtattcga ttgagatccg tctcaatacc
    14221 tcgtgtgtgc agactcatgg ttcccgtcac tccatagaat tcctgcggaa acgatccaac
    14281 aggtgacctt gcctctgatg gagagtaggg tgtgagtggg tataaaaaac cactgatggt
    14341 cccatgtgat gcgggtggtg ccatggcagg atgaacaaaa accctttcct tttccttttc
    14401 ataccatggg tgcggaatta ttctgaacga aaaattacgc tctcgaaaag aaccgtccgg
    14461 actgcatggg tgtgcgaact gacatcagaa cgggcaggat gagatgaatg agagttgccc
    14521 ttttatcgat gagatggggt accatggcca cctgtagccg aacggtatgc atcatatcat
    14581 ttctgtcaag ggaggttccc catcaaatgg gtgaccgtga cctcgctgaa agttcatcca
    14641 cgattcactc ttttgtccac cagatcaccg cagtacgacg gatcgcggag tgcaccagcc
    14701 tgccagaggt gtcccggcgc aacacacgtg agaagaaatc gatgaccggc gagaggtcct
    14761 cgagtcccgg gtagtgcagc gttacccagt ggcgggctgc ttcttcggct gactcgtagg
    14821 tggcttccca gatggcggga tatatccgga cattcgcata gatcccctcg tcatggagga
    14881 tattcagggg gaactggtag tcgggatacc ccttcttcag gaccccgtat tccccgaaca
    14941 cggcgcggta cagcgccatc tcctccgggc ctctccactc tcccgcgtgc cagaagaggt
    15001 agacggagcg tttcgcagcc gcatcgagtt tcagtagcgc agccttcagg tcataaaagc
    15061 caagggaaaa cgctgcgata gcaacttcat ggggccagat atcctgcccg atgagggtat
    15121 cctcccagcg cttctgcacg caggagtaat ttgtccttcc ctcggcaacc atccgctctc
    15181 gcagtatccc gagcatgccc cctgatgcat cgagtgccgt cacgtgtgcg acacgcgcag
    15241 caatggggac cgcaagcctt cctgtcccgg cacccatgtc gaggacagta tctgacggct
    15301 gcaaatcgag gcgctcaagg tcccgcagag ttgcgctccg ttcctccctc gtgttcctgt
    15361 gatacgaagc ggccttcttg tcccacaccg atgcaggctc ctcgtctttg gcggtcctgc
    15421 ctggggagct tatgtggatc gccttccaca gttcgttcca gtcgatgatc cgatgcatgc
    15481 gaatacctct gcatcacaat agtatatgct cttggtatca ggcgagggag atgctgacac
    15541 agggcagaag cgaggttatt ggaatcgaca atatcgtgaa tcgatccgca taatacagat
    15601 ctaattcttc ggaaacaatc ttcacaatca acaggcatca agaaacggat gtgcacgcca
    15661 aattcagtgg aagtgtctgt tcgaagaaag gggtaaaagg acacattctc tctccactct
    15721 ctacagcgta agatttcata ggttcgggtt atgtggaata cgagtgaaag aaaaaatagt
    15781 atgagataga attgaccgtc agctttttta taagccttga taaacgcctt ctaagaatgt
    15841 ttcgcggttc gatctcccta tcaggcggtg tggattgacc ttctctgccg agcagcttga
    15901 cgcactacag gaactcacca atattggagc cgctcacgcg gcaaccgcac tctcgcagat
    15961 gctcgggtgt gagatcggca tgagcgtgcc tgagattaat atcgttgata ttggcaaggt
    16021 ggggacgatt ctcggcgagg aggtctccac catggtactc ttccagctcc agggagacat
    16081 ccccgaaggg ggttttttaa tccttcagtt ctccgatggc tcggcggtca ggaccgcgag
    16141 tatgatgtgc gggctacccg acacagaccg tcctcttggt gatttggacc agagcgcact
    16201 tcttgagacc ggaaatatca tgatctcctc cttcctctgt gcatcgtccg aactcctcgg
    16261 gatcatgctg ctcccgtcac ccccggtcct tgtgattgat atcgcacacg caatcatcga
    16321 gtccctgatt gcacagatga gggttgacgc tgacgatgtc attgtcttcc aaatcacgct
    16381 caaatctgaa cagcacggga tctctggaaa cattctcatt tttcttgacg aaccctccct
    16441 ccagaaaata tcccgcatgc tggagaagat actctctccc tcatgaatga ttcatacatg
    16501 tttccccctc tggcacacgc ctgaatgccg gaaaagttca aatccaagag gggattcagg
    16561 aatatatgat gatcccggaa tctttcgcag tagaacttgt tccctggaaa gaagcagtgg
    16621 cgctctcaaa ggacctcggc gagtgcatca gggggaagta tcatccggac cttgtcattg
    16681 caatcggcag gggcggctac gttcccgggc gggtggtgtg cgactccctg ctgatcacga
    16741 cgcttaccag tatcaagatc gagcactggg gtgaggcagc caattgctac ggcgaagcgc
    16801 acatccgcta cccgctttcg gtcgatgtag aggggcagga ccttctcgtc gtcgatgacg
    16861 tgactgatac cggaaagacg ctgattgcgg caattgaata tctgcagtcg cttcggcccg
    16921 ggagtataaa gaccggcgtg ctccatcata agaccacctc cacgttcatt ccggactttt
    16981 acgcgaaaat ggtccccgaa tggcactgga tcgtgtatcc ctgggctttg cacgaagacc
    17041 tggtggggtt cgtggagaag gtgatgaccg gaagatcggc aactcccgga aagatccggt
    17101 cggcactcaa taaaaggtat cacatgaggc ctggaatgga tgacgtggtc tttgcggcca
    17161 gggaactggt agagagagga atagccgagg agaccaggga tggctacagg ctgaaaaaca
    17221 tcctttaatc cttttctcta tccgtgagcg actctcggtg ttccggattg cccactccta
    17281 attttttctc ctcgtcccag ccaacagtcc tgtgcgctca acccccgttc aggcagaggt
    17341 ccagtgcccc gctctcgaac atctccgcaa aaattgcttc aggatcatcg gtctctggcg
    17401 ggacatacgt gcgtggccgg gtacccagga tgcgcctcat ggcagctttc acatccttgg
    17461 ggcgatatct gacaagttcc cgctgaacgg atttctggac cggtcttgca tggaagaagc
    17521 ggtggcattc cgggcagagt ataagcaatt ctttctgcat atcaggacga tcaccttccg
    17581 caccggtgcg gggatctatc acgtgcatct cgaggagcga aagggggtat gtctctccgc
    17641 aatgctcgca cgccccgcct gcagcaagtt tcagcagcct cgatttgacg ggatgaacgt
    17701 gtaggaattc ctggagatcc atgacttttt cgcctcaatc tactcttctt ccagctggcg
    17761 gcgtgcatcc ttcaactgtg caatcgcctc tcctgaaacc gtgggatacg caaggtcaag
    17821 ggattgtatt cgcgtggtga tgatgtctgc aatcagggtg cgtgcgaccc acttgtggtc
    17881 tgcggggatg atgtaccagg gggcttcctc cgtgctggtc atggtaagtg catcctcata
    17941 cgcttcctga taggtacccc agaacttcct ttcttcaagg tctgagagtg agaacttcca
    18001 taacttctcc ctgtcttcga gtcggtccag gagtcgtttc ttttgttcgt cttcagaaat
    18061 atggaggtaa aacttgagga ttacggtgcc attccgtata aggtgccgct cgaacgtgtt
    18121 gatatcctcg taacgggcgt tccagaactt cttcccccgt ttcccgggag gaaggttgac
    18181 cagccgctcc gggtgtacct ttaccaccag gacgtcctcg taataggagc ggttgaagat
    18241 gcctatctcc ccgcggtttg ggagggctct ccagtacctc cagaggaacg tgtggtcgag
    18301 ctcctcgtcg gtggggaccc ggaagctgtg caccttgcac ccctggggat tcaccccgga
    18361 catcacgtgc ttgatggtgc cgtctttccc cgcggtgtcc atcccctgga ggatgataag
    18421 gaccgaatag atattactcg cccacaggag ttcctgcgca gatgcgagct gcgcccggtt
    18481 ctcttcaagg atctccatgg cacgctcttt caccgcttct tttcctgcct ccttcagggc
    18541 atcgttctgg gcccagccgg tatagtactc gccaagcctg accttcattc ctggtttgac
    18601 ctggatgcgc ttgagatacc gttcgtttat cacctgtgtt catcccctgc gtgacatctc
    18661 gctggtgcag catttactct ctttgcaggg cgaaccttta tcacttggca tacagttggg
    18721 agaaatcggg gcggtcgttg tggaggagga attttcacaa gaagatcgca aatagtgagg
    18781 taataccaat cattcacggt ttttttcaca aatttgtcgt ggccgttgtc tgggagtcat
    18841 ttatttacca gaggaaaacg gtagtctggt tgtacgggtg agtggccggt ctcatgagaa
    18901 gagtgaggtg gccgccgggg gacgaaagga ccatgcgggt cgtcattgca ctgggcggaa
    18961 atgccctcct gaggaggggc gaaccaatga ctgcggaggt gcagcgaggg aacgtgagtg
    19021 ttgcggcaag gctgattgcc ccactggcag gtgaccacga actcgtcatc acccacggga
    19081 acggtcccca ggtggggctc cttgcgctcc aggcagcctg ttaccggccc gtggagccgt
    19141 atccccttga tgtcctcgat gcacagaccg agggcatgat cggctacatg atcgagcagg
    19201 aactggcgag caacctgccc gcatcgcaac cgtgcgccac gatcctcacc atggtcgagg
    19261 tcgagccgga cgatcatgcg tttctccacc ccaccaaatt cgtgggccct gtttacagcg
    19321 aggatgagaa ggagcaggta tcgcgggaga agggctggac ctttcgaagg gacggaaatg
    19381 cctggcgaag agtcgtgcct tcaccccttc cccggaggat cgtggagatc cagccgatca
    19441 ggtggcttct ttcccatggt gcgattgtca tctgcgcagg cggagggggt attcctgtta
    19501 tcgcggcccc cggcgggagg cgggtgctcg caggagtcga ggcagtgatt gacaaggacc
    19561 gcgcaagtgc gctgctggca gaggaactca aagcggatct cctcgtgatg gcaactgacg
    19621 tggagggtgt gttccttgac tggaataccc cttcggcgcg ccttatccat tccacgaacc
    19681 cggcaaggct ccggaagcat cactttgcgg cgggatccat gggcccgaag gtggaggcgg
    19741 cgtgcagttt tgttgacgcg acggggagcc gggcagcgat tggggcgctc tccgatatca
    19801 ggggactggt ccttggggat gcagggaccc aggtgtctgc ggcagggtca ggagagactg
    19861 aggcataggc atgtcggaat tcggggtata ttcggaagtc ggaaggctgc ggaaggtgct
    19921 cgtccacagg ccggacctga gcgcaaggag gctcacaccg gggaaccacc aggacctcct
    19981 ctttgacgac gtcctctggg tggagagggc gatggaggag cacgatgcct ttgtgcgggt
    20041 actcgaagag gagggggtcc gggtgtatcg tctcgcctca ctccttgcgg acgtgctcgc
    20101 aggcaaagag gcccggaggt gggtgatcga gagggccgta cacccgatga cagtcgggcg
    20161 ttccgcgtgc gggtcggtga gggcgtgcct catggaaatg gagcccggcg cactggcaga
    20221 gcacctcatc gggggactca cacggagcga gcttgagtgt atctatctcg aggggatgac
    20281 cagatcgtca ctccaggtcg cggcatcgga ccccgactcc ttcatcctcc ccccgctccc
    20341 gaacaccctg tacacccgcg acacttcttc gtggatatgc ggggggttca cactgaaccc
    20401 catgttctgg ccggcacgaa gggccgaggt ggttaacacc gcggcaatct atcgcttcca
    20461 cccgctcttc tcaaatggcg gtatccggac gtggtactct tgtctcgatc acgggaatgc
    20521 cgcgcctcct gcgtcatccg caacagcctc gatggaaggg ggggatatca tgccaatcgg
    20581 cagggatacg ctccttgtcg ggatgggcga gaggacggga gggcccatgg tcgaagagct
    20641 tgcccgggtg cttcttatgt caggcgcggt ggagaggatc atcgtagccc agatgagcca
    20701 cgatcgggcg cacatgcacc ttgacacggt ctgtaccatg cttgacgaga gtacggtcac
    20761 ggtattcccg aaggtcgtcg aaaaaatcag ggcattctcc atccgccccg gggaagggga
    20821 aaggatcttc gaggtgaccc gcgaggagga cttcctcggg gcagtagccg acgccctcgg
    20881 agtcgggcgt ctcctggtca ttcccaccgg cggcgatgag taccaggccg cacgggagca
    20941 gtgggacgac ggcaacaatg tccttgcaat cgcgcccggc cgggtggtgg cgtatgacag
    21001 gaatacctgc acgaaccgga acttcgagaa ggccgggatc gatgtcatcg agattgcggg
    21061 atcagagctc tcgaggggac gcggaggcgg gcactgcctg acctgccccc tcaagagaga
    21121 cccgatctga ctcctgaaat cactccccgg catctttgat ctcattgctt tttcatcacg
    21181 ggtgacagac acttctccag agaacgatga ctcccgtgct tctcccgctg tttcagaagc
    21241 ggatttttga tcgacggcag gaactggagg cagtgaggga gtacccggtg gagaagctcg
    21301 ctgatattat aggggaggtg ggattctcat gcacctactg tgcaaggtgc tgcacgaggg
    21361 cattcaacgg ccacgtcctc ctcctcgacg aagacgcggc gaggatccgc tcgatcgatc
    21421 ccaccgccct cgagccgcag ccggagtatg atctctgcga tcagtacggg acgttctacg
    21481 tgtcaggata cactctcagg gcaatgccgg gtgagagtgg ggaatgccat tttctcacgg
    21541 acggccggtg ccggatctat ccagaccggc ccttgatatg ccgcgtatac ccctacatgc
    21601 tccaccggga gccggatgag gtgggggtgg ttgactggcg gcaggtcagc ggccttgacc
    21661 agcacggcga ttaccattgt actgtcccgc gcgatattgc gagggagatc gcctgcgagg
    21721 tgaaggcatt tgaatgcgca gtgatttcac acgagattgc cttccttttg tatgcgggca
    21781 gttatttctc agtccacggc ctccgccatg tccggaagcg gtttgatgac ggaatgcggt
    21841 ccctccgcac cgggaccgcg gtcagagtaa aggtcttttt cggaggaacg tttgaatcct
    21901 gggtagtgcg gggaggaaaa tgcacgttgt cagatgaaaa aaggcagggg gtctgatcag
    21961 gagaagaccg agaaatcgtt cttgatcgcg atggcatcga tgatgggttt gagtgcggtt
    22021 tctgtgatcc cgaacttttc catgtacagc tggaggatct tctgctcgtt tcctgcgagg
    22081 acaccgtcag acatggcgag gtccgtcagg atcgccatgg ttgcaatcct ctgctgatca
    22141 ttgagcgcct ttgcgacgag atcgactgcc tcggggaggg tgatgccctg gagtatcttg
    22201 actgcagtat ccacggcatt cctgtcaccg cgtgcaattt tcgcaaggtc catcatctcg
    22261 gcctcgtcga tcacgccatc ggaggcgatc actgtgatcg cggacagcac gagtgctgat
    22321 ttcgggttca gttcaacggc cttgctccct tttaacttgt caaagagtcc cattctgttc
    22381 acctgcgtca gatgacgttc cttctaggta agtcgtgctt catcatctgc ctttcgcagg
    22441 ggctggaaaa cccgggcgtc gaagtaatca gggtgactcg ttgccaagaa gttctccgag
    22501 ccatcgcgtc tcgtcccagg tctcgaggat tgcctggact gccatggtga gagggacggc
    22561 gagcagcacc ccgaatgcac cgaggatcag ccaccagaaa aagatggaga ggaatatgac
    22621 gagtggcgag aggttgagct gataccctgc aaggtggggg aagagcaggt actccgcgac
    22681 ggtgttgatg attcccgctc ctatgagcac caggagagct gcggcgggcc ccatctccag
    22741 ccaggcgagc accatcgggg gaatgactgc aagccagaac cctacgtaga ccacgtaccc
    22801 gaggacaaac gcgagaaacc cccacaggat ggcgaagtcc acgccgatac acgcaaaaaa
    22861 aaggccgttt acgacgccgt ttgcgaggtt gacctcggtt cggaccacga ggtattccac
    22921 catctgcctt ccgaaccgtt cagcactctc cattctcccc gaaaggtgta caatgacccc
    22981 ctgcaatttc ctggtcagcc ctgccgactc ggccaggatg aagagcgggg tgaagacgac
    23041 gatgagtgca gcggtgagga ggtctccaag gagtgcgatg acacctcttc cggttgtcat
    23101 cagcaggtca atcgcatcgc tgggaagaag tgtgaggaga tccgcgaaag agacctgccc
    23161 gcattccgtt gcgcttggaa gaagagactc gaggccctcc tggtagagcg gcagttgact
    23221 ggcaagctgc aggagggaaa ccccgactgc caggacgaat cccgccagtg ttgtaaagta
    23281 aaggaggatg actgccacga ctgcaagacg gggagagaac cccttcttct cgagccagtg
    23341 cttcagcacg atgaagagga tagagataaa gcctgcacag aggacagggc caaggattgg
    23401 agaagcagcc ttcattccgg cgaggagtat cactccgcat gcaaggacaa tgagaatgta
    23461 ataaggccga aagagctgaa tgcccgtgta cacaggggaa aaagacgcgg tcgcgtgtta
    23521 tcaatcttgc cccccgggat tcaaaactcg cacatcagga atggcaaagg gggtgagagt
    23581 gaggaaataa gagagaaacg ttcatatact cctcattgag acgtaatctc tgcgagagaa
    23641 gtgtgaactc atgacagatg aagtgacttc agctgcattg aaggtggatg acgtgatgca
    23701 cctctttccc tggtggatcg tgctcctcca gggtatagta tccctccttc ttggaataat
    23761 gttccttgcc tatcctatgg ggaccctctt tgtaatggtg acgtttcttg gagcgtactg
    23821 gtttgtgagt gggatctttg ccctcgtgag ccttgccacc gacaagacca atatgggagt
    23881 aaaggtcgtg cttgcattac tcgggatcat cgcgggcatt ctcatccttg cctaccccta
    23941 ttacagcacc atcattgtcc cggagatttt tgtcatcatg atcgcagtct ggggtgtcat
    24001 gatggggtgc gtgagcctct tctcctcctt caaggggggc ggtatcgggg cagcagtgat
    24061 tggcgtgctc gcaatcatct tcgggtgcat catcctcttc aacccgttga tcacggtcat
    24121 ttaccttccg tttgtgctgg gcatatttgg tatcattggc ggacttgcag cgatattctt
    24181 ctcgtttcag gttaagagtg cggcaggggc ataaccccct ccgaatccgg ggaaccatat
    24241 ggaaggtccc cccgtttttc acagcaaggg atcgcccaag ttattcctat tttcatacac
    24301 gccggctacg gaaggggaag ttggttggcc gggaaaaaaa ttctccgtca tacctgcggg
    24361 ttgaatgaat ttttatggga ccggtaagag ataggtgacg catgaagaaa ctgattggac
    24421 ttttcactct ctgcatggtg gcctttatgg ttgcaggctg ttgcatgccg cctcccacta
    24481 cggaaggcat ccagaccata agccctactt caactgcacc tgaaccctct atcggacata
    24541 acatgatccc gcaacccacc gatgtcatcc ccccattcta ttcaatcacg gttcaggtgg
    24601 gcaagaacac ggtctctgtc gatccctaca tcacagtcag tttccgggga gggccggggc
    24661 aggagttcac ccagagcatg cacgcaatcg tgtaccggtc agacgggtcc actgaatccg
    24721 ggacggtcgc atacccccag gtgggatcag agatcatcct ttccgggacc acaggaactg
    24781 accgggttgt ggtggatgtc actctcgtga cgggagagac gtatcatgca tacgatgccc
    24841 tggtgccgtt caggaatctt taattcctga accctctttc acgggaggca cattgtattt
    24901 tgatatgtac ggtccgcacc cgttttgggt gcggtcaacc ggcagtctta tccgcggaga
    24961 gcgtggacat ctcctcgcgt gatagaaggc gattgcgtga acggttttaa agcctgtgtg
    25021 ctggtccttg gaatagttat gtgccttgct gccggagcgt ccggagccga gcccctgccg
    25081 ggaacaaccg cagcaccttc gactcttccc ggagcagacc agggattttt cctgatcgac
    25141 tcgctccccc gggacgcaga tgtatatttt gacaaaaact tcctcggaga gacgcccgtc
    25201 acggttgccg tgtctatcac gggaaagcct gcccacgaga tcgacatcat ccgcggcgga
    25261 tacgagccat ggagtagcgt atacgaggga aatccgcggc ccggccagac tgtcacagtc
    25321 atggccaccc tcatccatgc caccgcatcg ggggcaatcc aggtgacttc ttcgccatcc
    25381 ggcgccattt caaccatgga ccgcgctcaa accatggaga cgccatgtac ctatgccacc
    25441 gtcccgatag gagaccatga agtatctgtg taccttccag gctaccagac atactataca
    25501 agtgtcaacg tgcagaaagg cgagacagcg cacgtatcag ccgagctctc tttggtaaga
    25561 gcgggcgggg caatctctgt aagctccacc cccgcacagg ctgcggtgta tgttgatggc
    25621 atatacaggg gtgtgacacc caccaccgcg ggaaaccttg ctcctggcca gcattccgtg
    25681 aagatatcaa gatccggata tcatgactgg acaggtcagg tagagatcct ggccggcatt
    25741 gagtcagcca tcagtcccac acttgtgcga gatcccgagc ccgcgtccgg aaccgtgagt
    25801 atcacgtcct cgcctccggg agctgacgtg tatgcagatg gcgtgtatgt aggacagacc
    25861 cgcaccggaa gcccgctggt ctacagagag ggtcagcccg gcttacacga gctccacctg
    25921 gcaatgacag ggtaccagga ttacactgcc acgggagtcg tgagagcagg ggagaattac
    25981 gacattgtca tctcgcttgc cccggaccca caggcatcaa ccgggaaaat ctctctggtc
    26041 tcgatgccat caggagcaga cgtgtttctc aacaatgcac accgcggccc cacccctatc
    26101 accctgcatt ctttggaacc ggggtcgtac actctcctgg tgagactatc cgggtatcgg
    26161 gactggcagt cggatgtgcg ggtctctgcg ggagagactg tcgaaataaa ggccacgctg
    26221 attcaaaagc ccccgagtga tagaccccaa agcggtattc ctgcatactt aacgctggga
    26281 agccttacga ttgctgcatt actgtggttc agccgtaaat aataatcccc gaccagatgg
    26341 tgcatagggc catggagaat atttctccct gtcccccatt gcttcaatcg cggggtggag
    26401 gagggacagg taggtgcacc tcatcgtgaa aatccgataa tctggccact ttctgctcaa
    26461 ggacgcgctg gctggtgata tcctccacta tcccattgat gtgggagata gacccctcgg
    26521 gtgcaaatgt tgcaagcgcg gtgacgagaa catggatggg agaaccgtca gcctttttga
    26581 gtataatttc ccggtttttc acaaatccct gctgcatgag ttcggcgagc agatccgccc
    26641 tcccccccgc atgcatgaac atgtcactca ccgggatatc ctgcaggtca tcgagggtat
    26701 catagcccag gattcggagc agcgaagtat ttccccacac gaatcttcct tcagggtcgc
    26761 cggtgctgcg gtatactccc acgtttatgt tgtcgaccag ttccctgtat tgcatctccg
    26821 cacgacgcag gcgttcttcg gatttccggg cggtgatgtc ccggattatt gtagaggcac
    26881 ccatgatcgt cctccctttc gagtatatcg gggagacagt gagcgataca tcgatcagct
    26941 tcccgtcttt tttgatcctc tgggtctcaa aattcacgat atgctccccc cgccgcacgc
    27001 gctccaggat ggcggggatc tcacctttga gggatggtgg ctctaagatg cagacgtcgc
    27061 ccccttcgac ctcttcggcg gaatagccat aaatccgctc cgcagcaggg ttccacgaga
    27121 ggattctgcc atcgatggtg gcactgaata tcccgtcatg tgatgatctg aaaatgtcaa
    27181 aaaggagatt cctggtctgg accgcttcca gtcgcggagt cacatcctcg tatacgatca
    27241 gttgctgtcc attggacatg ccaacgggca gaaacgagat ctccttgaag agtccgtcct
    27301 tgcatcgcac cctgaactgc cgcggtcgga tctccccggg ccgggattct gcaagatcca
    27361 ttttccatgt ttcccttgca cgtttctgct catcaaggtc aggaaatgcc tgcatgaacc
    27421 actccctccc tgtcgggata tcctcctgtg tatagccgaa gagctcggtg aatctcgggc
    27481 ttaaatagat atagcggcct gaagaatcga tgatggatat aggatggggg ttcgtggtgg
    27541 caattctctt gaagcgttct tcgcttgttc tcaacgcatg ctcgaacatt ctcttctgca
    27601 gggcgaggga tgcgacgccg aggtaggtat caagacagcg ggagtagtta tgccattcgg
    27661 attgcggaag gattacctct atggctccat agagtgaccc ttcccacacc agccccgcgg
    27721 aacaggcgtc agtgtcccgg cgttcttgat caggaagagt gtccacggca atatatcctt
    27781 gagggaagat acgctgcagg agaaatcccc ctgggtgaac gagggagccc gagagaagag
    27841 cctcccggaa attgacaagg ctgccatcct tcccggcaag ggaacaggtg tgaacgtcag
    27901 ggtcgatagg accaggcaaa aaacctctgc cggaagaatt gcgcggtgcg gtggctttga
    27961 gcgtgcaggt ccatatacct gagtcaattt caaacactgc cgcgattgaa ccagggatca
    28021 tttcggtgat gtgcgaggcg atacactcat acatatccgc aacgggatcc attgccatga
    28081 attcccgcga tttcgaagca acgaacgcga gctccctcgt gcgtgccaca tccaggttgc
    28141 gattcaccag ctcttccgtg atgtcatgcc cgattccctg gtagacaagg ggcattgagt
    28201 ccttgtcata cagcccctgg ataacccaag tatgccatac aggaagtccg cctttgggag
    28261 agacccggaa cgaaacagtt gcagtcggtt gctcgggaga gagggtagca aggtgcgaga
    28321 ggaatgcatt agtgtcctct ggataaatca gggagaacaa agatccgtaa ctgtctcctt
    28381 tcagggaaag ggaaaaaaag gatgagaaaa catcgtttgc agagatgata gtcccgtcag
    28441 ggaggaactg ggccaccatc tcgggcatgg catccaccag ggccatgtat tggagtttgc
    28501 ttgttttcag ggcctccttt gagcgtatct cattcgtgat gtctgtgagt atacatacga
    28561 cgccggattt tccgttctcg aagacaatgg ggaccatctt tgtcttcagg aaaaaaggct
    28621 cgtcacccct gcatatcctg aaggcatggg tcgagaccac ctctttctgg ctatcttcca
    28681 ccaactgtgc acagatggcg gcgagcaggg gatggtcttt ggattttatg tccacccctt
    28741 tcagtgcttc cgacgtgcat gataaaaacg caaggagggg ggcattagcg gaaagtatca
    28801 ctccattgtt atcgaggacg aggaccatat cgtggatata atgcaggatg cttgtgatgg
    28861 gaacacgcct ggagagcgag tagacgcggg tcgtcccaaa tgcgtgctgc accacctgtc
    28921 ccgagaggga gagtgcatgc aagtcccttg aaacagagtt gcggtgacgg tgcagccgtg
    28981 cagatatctc gttgatactc aaaccctggg gatgagaggc gagcaggtcc ctgatatcgg
    29041 aaaggcgctc ttcagacagg tgattcatac atggcactcc agccaggcag gaaggggtat
    29101 ccggttttgc tgcattctcc tggcgcttct cttcattggg taatggatct ccaggcataa
    29161 aatggtattt tgccagactc ttctgcatgt cacgaatgcc gtgcattcat gtgagcagga
    29221 acgcgccggt atgcgccgtt cgctccttat atgcagtgct ttctggcaaa tgattaatat
    29281 ttgaaggaat tcatcagagt gtgccaaggg ggtgaaaccc cggcatgaat gggaggaaca
    29341 ctctgcatgg accatatcac gcaacataca ggaaaatcgg tattgaacat ctcccaccag
    29401 gcggattact ctttccgcgt gggcacggaa tctgcgcaca ggggagaata cctaagagcc
    29461 ctcgaacatt tcgagaaggc gctctcgtca gacccacatt ttgccatggc ctggcatgag
    29521 aaggggaact gcctggacga gttgggccgc tgcgatgagg cactctccag ttacgacact
    29581 gccatccagc tggacccgca tcacgctgag gcctggttca acaaggggct cacattgaag
    29641 aagatgggca gggagaaaga ggcctactcc tgtatgaacc atggagtgga cctggctctg
    29701 gggagataaa cagtcctctt ttttttcagt gagcctaaaa aaacttcact tctgaagcca
    29761 tcccacccaa gggtggcagt tttccaggtc atgaacccgg taattgaata gccttgctcc
    29821 agttgaacag aatagatgca agagtcgcgg atcctcacct aaaattgcct tgtattccct
    29881 ccactgcaga cgcgcacggt cctccagcat ttcaatgaaa aaacgagcac atccgggagt
    29941 ggggtgcccc actttcagga agcaccctga aaaaaattgc ctgggattct ggttgttttt
    30001 ttaattacgg tctgtgtgga ataccggccc aatagtctaa taagggcaag ttgctttttt
    30061 ttgctatcta ttatgaacat atgccgggcg gattctgcac tcataagtgc acggcgctcg
    30121 gcgagttaac tacattaagt taacataaaa aataagtcaa tatcacgttc aaaatttaaa
    30181 aataacgttt agatactttt attttctgtt ggcaataact gcccctggta tacaggtgtt
    30241 taaatgaacg caaagtatgt acagacgacc tgtccctact gtgggaccgg gtgctcgttc
    30301 aaccttgtag tgatggacgg gaaagtgaca gggaccgcac cttaccagcg ctcccccgtc
    30361 aacgagggga aggtctgccc gaaggggacc tatgcccacg agttcatcaa cgcgcccgac
    30421 aggctgacga aaccgctgat caagaaggac ggcaagttcg tcgaggccag ctgggacgag
    30481 gcgtatgacc tgatcgcaaa gaagttcaag cagtacaagc ccgacg
//
LOCUS       DAOQYR010000018        29296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_1866_length_29296_cov_8.480387, whole genome shotgun sequence.
ACCESSION   DAOQYR010000018 DAOQYR010000000
VERSION     DAOQYR010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 29296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29296
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1866_length_29296_cov_8.480387"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..901
                     /locus_tag="PKM87_03860"
     CDS             65..901
                     /locus_tag="PKM87_03860"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HNQ29235.1"
                     /translation="MDTTEAFFDKLIIQLAPTGMVPNKSDTPHVPITPEEIVEDTVKA
                     YKKGVSFVHIHARDEQGNPTYKKEFFYQIFDGIRKRCPGIIICATTSGRCDRDLSHRT
                     EVLELEPEMASLMLGSVNFPNKSSINTIDDIRFLAKAMERNGVKPELEIFEPGFIGMA
                     KHLQEKGYLSQPLHFNLLLGTPGGFPAGIRDLSYLVDSLPEGSTWSAAGIGKYQLNIN
                     SAAILMGGHVRVGLEDSIYYDYRKRILAKNEQLVSRIAAIANELGRDIASPKDAREIL
                     HI"
     gene            876..2738
                     /locus_tag="PKM87_03865"
     CDS             876..2738
                     /locus_tag="PKM87_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HNQ29236.1"
                     /translation="MREKYFTFNNVEIDNTGGINLEKLFPSVFDGVFKGCNSEKVAIE
                     GIERNPMTYGELSHLVKYTVTDLNEMGYGRGDRLALMLPNGPEMATAFLSVVSGFSCA
                     PLNPLYREKELGFYLSDMNASAIIVHPDLEPYVRGVAYSLGIQVIILNPAGDTAGMFS
                     LCGENQERTHETGFAEPEDIGLILHTSGTTSRPKLVPVTQSSIVASSVYMAEPLGLKE
                     IDKCLNVMPLFHIHGLIGAVITSLVSGGSVVCTPGFNAECIMKWIGEHRPTWYTSVPT
                     IHQYVLEQAVLHKDQARMSSLRFIRSSSASLPPTLKEGLEDVFGVPVVEAYGMTEAPH
                     QISTNPLPPRIRKTGSVGMPTGLEMAVLGSQGEILPPHMTGEIALKGSRLFRGYENNP
                     EANEKAFLNGWFLTGDLGHMDEDGYFFIDGRIKEMINWGGEKISPREVEEALLSNTAV
                     AEAIAFSIPDPVLGEKVGAAVVLKETQEINVNDLKKHVSGNLAYFKVPACFWIVDEIP
                     KGPTGKIQRIGMYERLKDRTVATGDQTGPDYAPPISKTEKILADIWTEVLKKDQVGRN
                     DSFFDLGGDSLLATMAVSRIAQAFELKISIASVMDYDTIARLGELIDRKLKYKR"
     gene            2735..6871
                     /locus_tag="PKM87_03870"
     CDS             2735..6871
                     /locus_tag="PKM87_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HNQ29237.1"
                     /translation="MTIPVIPADSDRFGTRASFAQERLWFIEALDPESALYNQLICYR
                     ISGEVDPALLQQSIAEVIRRHIVLRTNLFVVDGLVYQRSSSLIPELPTIDISDKNEGS
                     ALDEAARILKELARQPYDLANEPLIRLRLIRLGIDDLLLAFVTHHTIMDGWSMGILIK
                     EVTSIYDALASGLDAKTALPPVGLQYDQYSIRQRAAYDSGEYAEQLEYWMDRLNGDLP
                     ILELPFDNPRPALQTYDGKALTLVLPSDLRDALQKVGRKEKATLFMTLFSAFIVLLHR
                     YSGQGDIIVGCPIAGRNQFDVEGMIGLCVNTLAIRAAIVPNLSFRELLRHVRSRTLEA
                     YENQDLPFEKLVEKLHVRRSLNVPPVFQTLFQLRNFPAPPRRIGGCQIERFELEEVLV
                     KTDLTLEITDTPDGLSCRFEYPVALFDHDSIVRMAGHWQTLLKDIVKDPDKKISYLQL
                     LTEEEKYDMLQMGKGKKSNFPRENIASIFEARAREYPEAIAIRHSNERMTYREVDERS
                     NRLAGRLREMNVRKGDIVVLFMERSASMVIAMLATLKAGGGYLPISPGEPESRIIQMI
                     KSVHPRAAITDPGLLSRTEKFLKPVFTVDNGLLEKRPLCHEGDPGPDDLAYVMFTSGS
                     TGTPKGVCVPHRGISRLVLNTDYIQIYSNDTVGHLSDPAFDASTFEIWGALLNGATLA
                     VIDKDVALSPSGLKMEIKDSHIDVLFMTTPLFHAIVSEDPSALCGLRYLLVGGDVMNP
                     AHARAFLEHCNTARLLNGYGPTENTTFSTCHFTRPDDCSSDSIPIGRPIANSDLYILD
                     NAMHPVPVGITGEIYVSGDGVALGYLDSPGLTASKFLPDPYVPGQILYKTGDLGKFLP
                     EGEVRFIGRIDSQVKIRGFRVEMGEVTSAIESHPSVKQAVVTPYQGPGGMTSLACYLV
                     KIDGMPEVTVEELRKYVADMLPSHMIPSVFIWIDAIPLTSRGKINFSALPLPPTNNAP
                     SPPNASYDDRTALIASIWRKVMGLADIGLDDDFFSIGGHSLLAIRLISTIEERFSIQI
                     PISVIFTAPTIRQMSAVIKNLDDERHYRNLVPLQKEGDRPPLFCIHGVSGVLHEFGLF
                     SRKIGNNQPVYGIQSSGLDGKEQPLDSVETMASRYIEAIRKIQPRGPYHLLAYCAGAV
                     IAYEMACRLEADGEKPGFFGIIDYSAPKQEMKSSFWLLFMFISDNVGGALKHLNIFVT
                     ESRRKKQQYLKNLPGFIGKKIIAFPVQLSGMIKSQSMENAPGRVGPATVNSDTPGFIK
                     GNTTAPALYPDWVMRYPEPQGTVARTIFDAIEAYSPGEYTGKIVLFISTNMIRSSRRE
                     GRYRHDLAWGNISTGSIDVHFIKGDHLSIVLEEENILRITPIIRKEIEQAILHVKSHE
                     "
     gene            6864..7646
                     /locus_tag="PKM87_03875"
     CDS             6864..7646
                     /locus_tag="PKM87_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNQ29238.1"
                     /translation="MNRNGSDLQPGEIHLYFVDLRNYRCEYPVTILSPDERERARRYH
                     STNASLQYIHARCLLREVLSLYLDHNPGDIIFEYSKNGKPHIRGESLDGVQFNISHSG
                     DAVAIVVSRGRRVGIDVEKIRDDIPDLELAKRFFSYAEAAAISRVHGLKRAEFFLKIW
                     TRKEALLKATGEGIGGLDNSIDLINKCSISHNGILWQVHDFAVPSEYTSAFVVEGGEC
                     FIKKREVSFQGGLKVFYSVGRDFINNLSHDSSKHTRILHSPQ"
     gene            7829..9307
                     /locus_tag="PKM87_03880"
     CDS             7829..9307
                     /locus_tag="PKM87_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNQ29239.1"
                     /translation="MKDRRRIDLKAIAREAMEKYGFEPEFPKTVIKEVNAMLPDLSAP
                     GRHDIRDMRSLLWSSIDNYDSEDLDQLEYCEAGSRGEILVRVAIADVDHFVTKGSHTD
                     RHAAHNGTSVYLGVETFPMLPDRLSKGISSLLPGEDRCAMVVEYTVLTGGSVRNGGIY
                     RALVNNKAKLVYEEVGDWLEGAAPVPVSVSSVPGLEEQIRLQAEVTRRLRSRRMQKGA
                     LDLETLEAEPVMKGETVHSLVIQEQNAARQIIEEFMVAANGTMVHVLGGAKVPMIQRV
                     VRVPKHWDGIMETAAAYRYKLPKQPDSKALAKFLDRQRAADPVRFPDLSLTIVKLMGP
                     GEYVPFVPGDTPIGHFALAVVDYTHSTAPNRRYVDIINQRLLKAVLDGEAVPYSGHEL
                     GRLAEWLSDREKASQKAERFMRKVAAALLLERRIGETFDAIVTGAAEKGTYVRLLDPP
                     AEGRVVEGERGLRVGNKVTVRLLSTDPPRGYVDFACVKKPPR"
     gene            9349..>10110
                     /locus_tag="PKM87_03885"
     CDS             9349..>10110
                     /locus_tag="PKM87_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNQ29240.1"
                     /translation="MAGQVILVTGSSDGIGKAAARELAARDAEVIVHGRDPGKGRQVI
                     RELKSQVWNRNLDLVTADFTSLEQVRRMAAEVSSRYTCLDVLVNNAGTYEKQRSLTPD
                     GIETTFAVNSLAPFLLTHELLPLLRKGHLPRVVNVASIAHREIRQVDWENLQGEKRYD
                     AFEAYALSALAHVTLTFGIARRIAGSGISVNCLHPGIVATKMLRAGFPGIRGKAPSEG
                     AKLPVYLAISPDVEGITGEYFEESVQPTLPSDLSRS"
     assembly_gap    10111..10210
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10211..11592
                     /gene="pap"
                     /locus_tag="PKM87_03890"
     CDS             <10211..11592
                     /gene="pap"
                     /locus_tag="PKM87_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HNQ29241.1"
                     /translation="AHWDLRIPIVIVVEGWNASGITTVISELIQYLDPRGFVLHSIGS
                     PKDEERARPLLWRFWTRTPARGRMAIFARSWYSRSLAEQVSGIDWKSRIKRAIFDINT
                     FERQLTDDGVVIIKFFLHISKEEQRKRLNEREKDALTSWMITQGDWDFHAHYDDYLPI
                     IEDYLSGTDTRHSPWTVIGATDGNYTRFKVYSTVIRTLEEKQKILEKKRQEKCGPALA
                     KPPRNKIKRSAEAPRQYTRAEYEMILQECQEQVHDIQYLLFKRRIPLIIVYEGWDAAG
                     KGGNIMRLVRAMNPRGYDVVPIARPDQTELEHHYLWRFYRRFPAAGHITIFDRSWYGR
                     VLVERIEGFCTESEWKRAYHEINEMEEAYVEGGGGLIKFWLEVSREEQYRRFIARQND
                     VLKEWKMTEEDWRNREKWKEYENAVDEMLARTSTSIAPWTVIESDDKYYARLKAIQTV
                     ISYGSEALE"
     gene            11687..11881
                     /locus_tag="PKM87_03895"
     CDS             11687..11881
                     /locus_tag="PKM87_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29242.1"
                     /translation="MHDIVTTRKMENGVACYYGESGKEKFESFTYRELIDMKINALDL
                     LDDPRNYAVDTEKHTLVMKK"
     gene            complement(12158..>12396)
                     /locus_tag="PKM87_03900"
     CDS             complement(12158..>12396)
                     /locus_tag="PKM87_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29243.1"
                     /translation="AFFLASVVFIRLTYHDYWRGLRHLAAALCIGGVLSVILGFFWGG
                     IPLDQLLSLSYFTTDALVALITGLALSLFMGSMR"
     assembly_gap    12397..12496
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12497..12792)
                     /locus_tag="PKM87_03905"
     CDS             complement(<12497..12792)
                     /locus_tag="PKM87_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29244.1"
                     /translation="MVDLSPITYVQVRATEYVSTLPDEVLRGNVFAIVIFLFLLYIAI
                     YIIQKFTTLLVFVLKKVFLLIIVVLAFYEFARIFVLKIAIEGLTADTIIFGIAGT"
     gene            complement(13140..14060)
                     /locus_tag="PKM87_03910"
     CDS             complement(13140..14060)
                     /locus_tag="PKM87_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HNQ29245.1"
                     /translation="MIIPLILFSAGLVLLLKGADLTVEGAEGIASRLGISATFIGLTV
                     VAFGTSLPELVVTSEAFRAGNFGIGTGNIIGSNISNIALILGLYAVLAPPAFRVHLSR
                     QRFMLHTALMLSATVAFALLCWRSVLDLLSGIVMLALFAALVVILWRSGHVMERAAEK
                     DSPYSLLFTIGGLAAVIIGAHLLLTGAVEIAALFAIPPVVIGLSMVAVGTSLPELATT
                     VVAAYRNRPGIAIGNIIGSNIFNLLFVMGINALFIPIPPPEMVTIGPLLLFSMAILPL
                     FSGKMTVTRAWGALLVGGYLVYILSLYWVF"
     gene            complement(14178..14822)
                     /locus_tag="PKM87_03915"
     CDS             complement(14178..14822)
                     /locus_tag="PKM87_03915"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HNQ29246.1"
                     /translation="MTLVRLGVVAVLLLTLAITAIVYPALPDQVPSHWNAAGEVDGYL
                     PRLWGAAIVPLIMIPLTALFFLIPRIDPLRENYRKFQPYYEGFILVFALFFAIIQVQI
                     LLWGIGIEISPNIVIPPLFGALFIYIGFLLDHAEPNWFVGIRTPWTMSSEKVWKKTHA
                     MGGRLFKIAGIVSIVGVFFGSFALWFCLVPVLLVAGYTVVYSYIQFQRERISTG"
     gene            14940..15269
                     /locus_tag="PKM87_03920"
     CDS             14940..15269
                     /locus_tag="PKM87_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29247.1"
                     /translation="MTGFRRSHLRLLGMAVLAGAGFRLGIPGLAEVIEGIWSHAEPAS
                     RALLPLELQGMIGIFGILVLGIVIYLNYEIFMKGAKRGRRGLLITVCGFCAGILLANI
                     VIAALFQ"
     gene            15368..16285
                     /locus_tag="PKM87_03925"
     CDS             15368..16285
                     /locus_tag="PKM87_03925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ29248.1"
                     /translation="MRAGLRMLDHNREKFGIILLNWNAEELTTRCVRALQEWHKLRPH
                     IYVVDNGSSGTGLYFEDSRDVPISLIKSPKNRGYAGGNNLGIRKAIEDGCEYLVLLNS
                     DAYISENNMEILLDQFRRYDDIGCLGPGICEGDLLFLGGRDMGLHPNTRNTLSQETSM
                     KGIMYVDYVPGMVFLTTKDVIKRIGFLDEQFFFSGEIADFCKRVLKAGYRCAVCPDVR
                     ANHVVVEDNPLRSSLYAYYSFRNRFLFVRKHHSNIRWILEPFWILWGMHKYVRGVILG
                     LDNESAAYRMAIMDGVRGVFGDRNDLFSV"
     gene            16266..17216
                     /locus_tag="PKM87_03930"
     CDS             16266..17216
                     /locus_tag="PKM87_03930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ29249.1"
                     /translation="MTSFPYSASIIIPNWNGMNWLPGCLAALKDQTVQDFEIIVVDNG
                     SSDGSVPYIQSRYPAVRIITFRKNCGFSKAVNEGIKKASGKYIVLLNNDTLPEKEWLI
                     SLLNEIRAAPIEVGSLASLMIRMDNPSLTDDAGDYLDWYGLAFKRGNGEPIFQWIENE
                     PVFSACAGAVLYRREFLERTGLFDEKFWSYLEDVDLGLRGNLLGFTCIFVPSARVLHK
                     SHGSAMPSGAYVYHVTKNRVMLLLKNIPSILLAQNARTLISGQILTLRINKNPSSSIR
                     AYLYIVFLLPKILHDRRCFLPRVVLSIDAINTLLRKTGNQ"
     gene            17357..18508
                     /locus_tag="PKM87_03935"
     CDS             17357..18508
                     /locus_tag="PKM87_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011450005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ29250.1"
                     /translation="MEKDIEITVIIPTLNEEISIHRCISKILAVFREHDLEGEIIVSD
                     SSTDRTPEIAASLGAMVVRPEQKGYGAAYLAAFPHARGKYVVIGDGDDTYDFSQIPKL
                     VEPLKHGADMVIGSRFNGKIRPGAMTPLHQYIGNPILTWMVNVLFHADFSDVHSGFRA
                     IRREALERLDLRSPGMEFASEMMVRARQKNLVIEEVPITYYPRQAPSKLHSFADGWRH
                     VRFVLLLNPLPFIAIPGAIFAFVGFLLMILFALWGQVTTSHLHSFILGAFLLCGGVQI
                     VVIGVLIKIYSVTHRYEEKAGIIVSIMNYHSLERFLLVGGLILSLGFLSGLSVIIAWI
                     QSGFGQIAEIVNAVAALCLGTIGLQIIFMAIFTSMMLLREENGDTKNPA"
     gene            18505..19665
                     /locus_tag="PKM87_03940"
     CDS             18505..19665
                     /locus_tag="PKM87_03940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNQ29251.1"
                     /translation="MKIAFVYDMVYPFRIGGVEKRVWEISRRLSARGHEVHVFGLKMW
                     EGPPDTFREGVYLHGVCRPMPFHTGESGRRTIFPALWLSLFLFLPLLRKGRFDVIDCQ
                     NFPYFPCFPAGVVAALRKSLFLVTWHEVWGRYWFYYMGRAGSVGIFIERLVKNLTRCH
                     IAVSPTTKAMLLDLGVEEPVAVVPNGVDFERIRKVPPSSEQSDIICIARLIPDKHVDV
                     LIGAVALLKASIPEICCTIVGRGPEEERLNAQVSLSGLDKNIRFTGFVKDQDDILALL
                     KASRACVLPSTREGFGMVALEALACGIPVVTADHPGNAIRDLAEMGGVRAVPLTAQDF
                     AVAIQETLQSPLSTFVPSLEGAWDWDAVTEQWLGMAEEFLRISKEHAAKGSL"
     gene            19706..20977
                     /locus_tag="PKM87_03945"
     CDS             19706..20977
                     /locus_tag="PKM87_03945"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNQ29252.1"
                     /translation="MRISIIGAGYVGLVTGACFAELGHEVHLVEIDREKAGVIDRGIA
                     PFYEPGLEALLKKHIHRTLAVSCEYQEITGSDCTFICVGTPPSPDGSADLRFIRSAAH
                     SIGTALKGGRDPHTVVVKSTVPPGTTRSIVAPAVMEASGRHDGPITFAMNPEFLREGR
                     AVEDFFHPDRIVIGCSEDIACRKVAALYDGIDAPLVTAGLDAAEMIKYVSNAFLAAKI
                     SFSNEVGNICKKLGIDVYEVMKGVGLDTRISAHFLNAGAGFGGSCFPKDVSALISLAE
                     SMGENPRILKSVMEVNDSQALRMVTMLEARIGNICGKQVAVLGLAFKDDTDDVRDSRA
                     IPVIQSLVDRGALVRAFDPIANEAMSRIFPGINYYGSAEEALTSADACLVMTEWPEFS
                     QLDLEFDRMKSRVIIEGRRILSCGGKEGICW"
     gene            21142..22041
                     /gene="galU"
                     /locus_tag="PKM87_03950"
     CDS             21142..22041
                     /gene="galU"
                     /locus_tag="PKM87_03950"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HNQ29253.1"
                     /translation="MKHQHVSKVVIPAAGLGTRFLPMTKAQPKEMLPVVDKPVIQYVV
                     EEAVASGIDDILIITGRGKRAIEDHFDRSFELEHRLKESGNLELHSETEELSQLAEIH
                     YIRQKEPKGLGHAILCAERHCNGEPFAVLLGDTITVPYPGEKPCTAQMVKAFAEWNSS
                     IIAIEEVRKEKIQDYGIIDGTEVRSGLYRIDAIVEKPKPADAPSNLASIGRYVLTPDI
                     FDLLHGTRPGYGGEIQLTDAIQQLPTKMGLVTRCTRYDIGDKLGWLKANLEFCLQREE
                     FNDEVLGFLTSLQTKNRVKHPEK"
     gene            22576..28041
                     /locus_tag="PKM87_03955"
     CDS             22576..28041
                     /locus_tag="PKM87_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HNQ29254.1"
                     /translation="MDSCTAFHGICRTERDNKASGEDGVAMEGKAAFIILCVLLTTGI
                     SGTLAQNNTSLVFLENESNNSLQIDPVILNSTVFHGYDTGSEDDEDVGNPYPVDMSES
                     STEDQGGPGGIVTGEAVEGEGSSNGNLTNGKEKKDKEKAQSSEDEIIIRFRVEPGKEM
                     SAGAAETAHKDASTKAKKDSKVKEDFRVKNDFDKKGLPGTQVVKVPPGLSVDEAIALY
                     SENPDVLSAEPNHRIELMSIPDDPGFSFQWGLLNAGNPGADIRAVSAWNISTGSSGVL
                     IAIPDTGVDISHPDLTSNIWSNSREIPGNGLDDDGNGYINDVNGWDFVNNDSSPVDDN
                     GHGTHVAGIAGAVGNNGLGISGVMWNTRLLPLKVIGASGYGYESDAIEAVLYAQQAGA
                     QVISISWGSYGESQALKDAIAGYPGLVVCAAGNSAQDNDLYPLYPASYPSKNIISVTA
                     TDEQDSLAPFANYGAVSVDIAAPGVGIYSTVPGSGYDMRSGTSMAAPYVAGVAGLVLS
                     VNGDLDGAQLADILIASADRLSSLEGRISSSGRVNAESAIVLATGQTNIPPTPSPTIT
                     VTPTLTPVMPEGEPQLAPLNPEFVAYIQSHTANTSVTTDEGFGLGYIPPPLDMTWLEG
                     QVLHISDNMILSLPPSYSLKTLGCVTSVKSQGSCGSCWAFATYGSAESVLMPEEAWDF
                     SEDNLKNLHEFDYGPCSGGQYYMSTAYLVRWSGPLTEAADPYSDSPTSYSPTGLLPVK
                     HVQNVIFLPSRGTALDNDNIKNAIMTHGGVSTTFYWASGSYNSNTKAYFSTYSGENHG
                     VTIVGWDDSYSKSNFLTSPAGDGAFLAKNSWGPGWGNEGYFWISYYDTSLGKSLNAVF
                     LSEPAENYDYIYQYDPLGWVSSVGYGTNTAWGANKFTSARDEQLEAVGFYTASPNTAY
                     EVYIHRNPTDGPLSNTGAVSSKTGIIDVPGYHTIPLSNPVSLNSGDTFSIVVKITTPG
                     YAYPVPFEYPTGGYSSQATSKAGESYISPSGASGTWVDLTDWRSNSNVCIKGYTTTWK
                     PPVANFTATPVTAIVPARIQFNDTSTNVPTSWQWKFGDGNTSSERNPVHTYEGVGTYD
                     VNLTVWNAYGSDSVTKTDYITVVEAPVFLEGWTYRKLVTIAGSPDGVLTDYQMRFVVH
                     QGEGIDSGEDVYVGTTVKEDYSDLRFTTIGNAPMPYWIEASDAGSAVVWVKVPEIPVT
                     GTQVYVYYGNPGAEAVSDGEATFILYDDFSGSSLDTSKWTNMLGSGVTVSGGVLTCAA
                     STSASYGVESKNTDLGVNISIRAKMYPFHPKSTTLLEGLYARHGPTTPYQNALYFSHV
                     VSSYSGKYLSRNGGSVTTESVTGLGGAGWYILEGRMDSSSIKWSLNDANPVTISTNYY
                     PTSSALRIVSYASGAKISTDWIFVRKYSATEPSVIDWTQQEQAEIAPVAAYCSNVNEG
                     SKPLTVQLTDLSLFQPTFWQWDFGDGNTSSERNPVHTYEGVGTYDVNLTVWNAYGSDS
                     VTKTDYITVVEAPVFLEGWTYRKLVTIAGSPDGVLTDYQMRFVVHQGEGIDSGEDVYV
                     GTTVKEDYSDLRFTTIGNAPMPYWIEASDAGSAVVWVKVPEIPVTGTQVYVYYGNPGA
                     EAVSDGEATFILYDDFSGSSLDTSKWTNMLGSGVTVSGGVLTCAASTSASYGVESKNT
                     DLGVNISIRAKMYPFHPKSTTLLEGLYARHGPTTPYQNALYFSHVVSSYSGKYLSRNG
                     GSVTTESVTGLGGAGWYILEGRMDSSSIKWSLNDANPVTISTNYYPTSSALRIVSYAS
                     GAKISTDWIFVRKYSATEPSVIDLPSFNIQHLKIIER"
     gene            28110..29180
                     /locus_tag="PKM87_03960"
     CDS             28110..29180
                     /locus_tag="PKM87_03960"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HNQ29255.1"
                     /translation="MNFPGNERLFIGNMKDCYLGYSNYQSIRKHCASGGIISNFLIFL
                     LDCKIIDGALVCRSKVIDNKISYEVKIVTCSEDILDYASSVYFNIPLLEYIPMIKKFD
                     GKIAFVGLPCQIRILRNLMKNDIILREKVFLIIGLFCGHNSRRNLLIDVLNQKGIREF
                     DVKEIIFRQGLWRGNMQVTLKDDKKISFPFQDFSIYQNLHFDSLKKCLFCVDHTAEDA
                     DISCGDAWLSYLRNDPIKHSLIITRTDFSNYFIHEMRRKGDIFLMQIEPEIIYKAQKR
                     SLISHKSIEARSKIGKIFGYSITYPSEYYNTARWNDYLASFILIINIQISSNEKFRKY
                     IFFIPKIILYPYLFLFKSLTNF"
BASE COUNT         7624 a         6615 c         7771 g         7086 t
ORIGIN      
        1 ttttctgttc aattacgatt atttattact gactcagtaa gaataatcta aataaggata
       61 atagatggac acaaccgaag ccttttttga taagcttatc atccagcttg ctccgacggg
      121 gatggtgccg aacaagtccg acacacccca tgtgccaata actcctgaag agattgtcga
      181 ggatactgtc aaagcgtata aaaaaggagt ctctttcgtc cacatacatg caagggatga
      241 acaaggaaac ccgacttata agaaagaatt tttttatcaa atattcgacg gaatcaggaa
      301 aaggtgccct ggaatcatca tctgcgccac gacgagcgga agatgtgaca gggacctgag
      361 tcaccggaca gaggtgcttg agcttgaacc tgagatggcg tctttaatgc tcggctctgt
      421 gaactttcct aataaatcga gtatcaacac cattgatgat atcaggtttc tcgcaaaggc
      481 aatggaaaga aatggtgtga agcccgaact tgaaattttt gaaccaggat ttatcggtat
      541 ggcaaagcac ctgcaggaga agggatattt atcccagccc ctccatttca atctgctgtt
      601 gggaaccccc ggaggattcc cagcagggat tcgagacctt tcgtatcttg ttgactcatt
      661 gccagaaggc tcaacatggt cggcagcagg tattggtaaa taccaattga atataaacag
      721 cgctgcaata ctcatggggg ggcatgtccg ggttggcctt gaagattcca tttattacga
      781 ttatcgaaag cgtattctcg ctaaaaatga acaattagtg agcagaatag ccgcgattgc
      841 aaatgagctt ggcagggata ttgcttcacc aaaggatgcg agagaaatac ttcacattta
      901 acaatgtaga gattgacaac actggaggga taaacctgga aaaattattt ccatcggtct
      961 tcgatggggt ttttaaaggg tgtaattcag aaaaagttgc cattgaagga attgagagaa
     1021 atcccatgac ctatggcgaa ctgagtcacc tggtgaaata tactgttaca gatttgaatg
     1081 aaatggggta cgggaggggg gatcgtcttg cattaatgct cccgaatggg cctgaaatgg
     1141 cgactgcttt cctgtctgtt gtttctggat tcagctgtgc tccattgaat cctctataca
     1201 gggagaaaga acttggtttt tatctttccg acatgaatgc ttcagcgatt attgtccacc
     1261 ccgatttgga gccgtatgtg cgtggagtag catattcgct aggaattcag gtcattatac
     1321 tcaatccagc tggcgatact gcaggtatgt tcagcctttg cggtgaaaat caagaacgga
     1381 cgcatgagac cggatttgct gaacctgaag atattgggct tatactacac acctccggga
     1441 caacatcccg tcccaagctt gtgccggtga cccaatcgag catcgtcgcc tcgtcagtat
     1501 acatggctga gcctctgggt ctgaaagaaa tcgataaatg tcttaacgtg atgccactgt
     1561 tccatattca cgggctcata ggcgcggtaa tcacgtcact tgtgtcaggc ggcagtgtgg
     1621 tttgcacacc cgggttcaat gcagagtgta ttatgaagtg gataggtgaa catcgtccga
     1681 cgtggtatac atcagtcccc actatccacc agtatgtgct cgagcaggca gttttgcata
     1741 aagatcaagc acgaatgtca agtctgaggt ttatcagatc ctcttcagca tcgttgccgc
     1801 caactttgaa ggaaggactt gaggatgtgt ttggtgttcc ggtagtcgag gcgtatggaa
     1861 tgactgaagc gccacaccaa atctctacaa atccattacc tccccgtatc cgtaaaaccg
     1921 gctctgtggg gatgccgaca gggctggaaa tggcagttct cggtagccag ggagaaatat
     1981 tgcctccaca catgacaggc gagattgcgc tcaagggttc tcgcctgttc aggggatacg
     2041 agaacaatcc cgaggcgaat gaaaaagcgt ttttgaacgg ctggtttctc acaggtgacc
     2101 tggggcacat ggatgaggat ggctattttt tcatcgacgg gcggattaaa gaaatgataa
     2161 attggggggg tgaaaagata tcaccacgcg aggttgaaga agccctcctg tcgaacacag
     2221 cggtggcaga agctatcgcg ttttccatac ctgatccagt gctcggagaa aaggtcggag
     2281 cggcagtggt attaaaagaa acacaagaaa taaacgtaaa tgacttgaaa aagcatgtgt
     2341 ccggcaatct ggcatacttt aaagtcccgg cttgtttctg gattgtcgat gagattccca
     2401 aagggcctac cggaaagatc cagagaattg gcatgtatga aaggctgaaa gatcggacag
     2461 tcgcgacagg tgatcaaacc ggaccagatt atgcgcctcc gatatcaaaa actgaaaaaa
     2521 tactggcgga tatctggact gaggtgttaa aaaaggatca agtcggcagg aacgacagct
     2581 tttttgacct tggcggagac tcactcctcg cgaccatggc ggtttcccgt attgcccagg
     2641 catttgagtt gaagataagc atcgcaagtg tcatggacta tgataccatt gccaggcttg
     2701 gagaactgat cgatcgcaag ttaaagtata agcgatgacg attccagtaa ttccggcgga
     2761 tagtgacaga tttggaacga gggcctcctt tgcacaagag aggctctggt ttattgaggc
     2821 tcttgaccct gaaagcgccc tctataatca actgatttgc tatcgcatca gcggtgaggt
     2881 tgatccggct ctattacagc agagtatcgc agaagtcatc cgacgtcata ttgtactcag
     2941 gacgaatttg tttgtcgttg atggcctggt ttatcaacgc tcctcatcac tgattcctga
     3001 actacccacc attgatattt cggacaagaa tgagggtagt gcactcgatg aagcggctcg
     3061 tatattaaaa gaattagcca ggcagcctta cgatctggcc aatgaaccat tgatccgcct
     3121 gcggctgatt cggctcggaa ttgatgatct cctgcttgcc tttgtaacgc atcacacaat
     3181 catggacggg tggtcaatgg gcatcctgat aaaagaggtg acatccatat atgacgcgct
     3241 cgccagtgga ctggatgcca aaacagcact gcctcccgtc gggctccagt acgaccagta
     3301 ttcgattcgg cagcgtgctg cctatgattc aggagagtat gccgagcaac tggaatactg
     3361 gatggacagg ctcaacggtg atcttcctat actcgaactc ccatttgata atccccgccc
     3421 ggcgctccag acatatgatg ggaaagcctt gacccttgtc ttaccctctg atctgagaga
     3481 tgcgcttcaa aaagtgggac gaaaggagaa agcaacactc tttatgacgt tgttttcggc
     3541 gtttattgtg ttattacacc gatattcagg gcagggagat atcatcgtgg gctgtcctat
     3601 cgcagggagg aaccagttcg acgtcgaagg gatgattggc ctgtgtgtga atacactggc
     3661 aataagagcg gctatagttc caaacctgtc gttcagggag ctactcaggc atgtcaggtc
     3721 aaggacactg gaagcatacg aaaaccagga cctgcctttc gagaaacttg tggaaaagtt
     3781 gcatgtcagg agatcgctaa atgtcccccc ggtttttcag acactattcc aactcaggaa
     3841 cttccctgcc ccgcccagaa gaattggcgg atgccagata gagcggtttg aactggaaga
     3901 agtgttagtc aagacagatt tgactctcga aattaccgac acaccggacg ggctttcatg
     3961 cagatttgaa taccctgttg cgttatttga tcatgactct atcgtccgga tggctggaca
     4021 ctggcagaca ttgctgaaag atatcgtaaa agaccctgac aaaaagatat catacctgca
     4081 acttctcacg gaggaagaga aatacgatat gttgcagatg ggaaaaggta aaaagagcaa
     4141 ttttcccagg gaaaatatcg cgagtatctt cgaggcaagg gcaagagagt atccggaggc
     4201 aatcgcaatc aggcattcaa atgaaagaat gacctatcga gaggtcgatg aacgctccaa
     4261 ccgtcttgca ggccgactaa gagaaatgaa cgtgaggaaa ggcgacatcg tcgtgctctt
     4321 catggagcgt tctgcgagta tggtcattgc catgcttgcg actctgaaag caggtggtgg
     4381 ttatttgccc atcagcccgg gagagcctga aagcaggatt atccagatga ttaaaagtgt
     4441 acacccacgt gctgcgatta ccgatccggg actgctttcc agaactgaaa aattcttaaa
     4501 gcctgtgttc acagtcgaca atggattact ggagaagcga cctctctgtc atgagggaga
     4561 tccaggacca gatgacctcg catacgtgat gttcacgtct ggatctacag gtacgcctaa
     4621 aggggtgtgc gtacctcacc gcggaatctc ccggctagtc ctgaatacgg actatattca
     4681 aatatattcc aacgataccg ttggtcacct gtcagatcct gcattcgacg cttcgacatt
     4741 tgaaatatgg ggagcgctct taaacggtgc taccctggca gttatcgaca aagacgtggc
     4801 attgtctcca tcaggattaa aaatggaaat aaaagacagt catatcgatg tgctgttcat
     4861 gacgacgccc cttttccatg cgatcgtctc tgaggaccct tcagcgctct gcggcctccg
     4921 ctacttgtta gtagggggag atgtcatgaa ccccgctcat gcgagggcgt tcctggaaca
     4981 ctgcaatacg gcgcggttgt tgaatggata cgggcctacc gaaaacacta cattcagcac
     5041 ctgccatttc acgaggccgg atgattgctc cagcgacagt atacctattg gccgtccgat
     5101 agcaaattcc gatctctata ttctcgacaa tgcgatgcat ccggttcctg tgggtattac
     5161 aggagaaata tatgtttccg gggatggtgt tgctttggga tacctcgaca gccccgggct
     5221 tactgcatca aaatttttac ccgatcctta cgttcccggt cagattcttt ataagactgg
     5281 cgaccttggg aagtttctcc ctgagggtga ggttcggttt atcggtcgaa tcgacagcca
     5341 ggtaaaaatc agggggttca gggtcgagat gggcgaggtc acatctgcta tcgaatccca
     5401 cccctcggtc aagcaggcag tcgtaacccc ttaccagggt cctggaggaa tgacatcatt
     5461 agcctgctac ctggtgaaga tcgatgggat gccggaggtg acagtcgaag aactgcgaaa
     5521 atatgtcgca gacatgctac cttcccacat gataccatct gtcttcatct ggatagatgc
     5581 aattcccctg acctcgagag gtaagatcaa cttcagcgcc ctcccactgc cccccaccaa
     5641 caatgccccc tctccaccaa acgcgtctta tgacgatagg actgctctta tagcttccat
     5701 ctggaggaaa gtgatgggcc ttgccgatat aggattggac gatgattttt tcagtatagg
     5761 tggacattcg ctgcttgcaa tcaggcttat ttcgactatt gaggagaggt tttccatcca
     5821 aatccccatc agcgtaatct tcaccgcacc tacaatcagg cagatgtctg cggtgattaa
     5881 aaatctggat gacgagcgtc attaccgaaa tctcgttcct ctacagaaag aaggcgacag
     5941 gccgcccctg ttttgtatcc atggtgtctc gggagtgctg cacgagtttg gtcttttctc
     6001 acgaaagatt ggcaataacc agccggtcta tggtatccag tcatcggggc ttgatgggaa
     6061 ggagcaaccg cttgatagtg ttgaaacgat ggcatcccgg tatatcgagg caattcgaaa
     6121 gatccaaccc aggggacctt accatttgct tgcatattgt gcgggtgctg taatagcata
     6181 cgaaatggcc tgcagacttg aggctgatgg agagaaacca ggattttttg gaataataga
     6241 ttattcggca ccaaaacaag agatgaagag ttcattctgg ttattattca tgttcatctc
     6301 agataacgtg ggcggtgcac tgaaacattt gaatatattt gtcactgaaa gccgtagaaa
     6361 aaaacaacaa tatctgaaaa atctcccggg ctttattggt aaaaagatta ttgccttccc
     6421 ggtgcagttg tccgggatga tcaaatcaca gtccatggaa aatgcaccag gacgcgtggg
     6481 gcctgcaact gtcaattcag acaccccagg attcatcaaa ggaaatacga ctgcaccagc
     6541 tctgtatccg gattgggtaa tgcgctatcc tgagccgcag gggaccgttg ccagaaccat
     6601 ttttgacgcc atcgaggcgt actctccagg ggaatacacc ggaaagatag tgctgttcat
     6661 atcaacaaac atgatcagat cttcaaggag ggaaggcagg taccggcacg atttggcctg
     6721 gggcaatatt tcgacgggat ccattgatgt ccattttatc aaaggggacc atctttcgat
     6781 cgttctcgaa gaagaaaaca tactccgaat cactcccatc ataaggaagg agattgaaca
     6841 agcgatatta catgtaaaaa gccatgaata gaaatggaag tgatcttcaa cccggagaga
     6901 tacacttgta ttttgtcgat ttgagaaact atcggtgtga gtatccggta acgattctta
     6961 gtcccgatga gcgggaaagg gcaagaaggt atcattcaac aaatgcctcc ctccaataca
     7021 tacacgcacg gtgtttactc cgtgaagtac tctctttgta cctggatcat aacccgggtg
     7081 atattatttt tgagtattca aagaatggta aaccccacat tagaggtgaa tcccttgatg
     7141 gtgtgcagtt caatatctcc cattccggag acgcagttgc tatagttgta tcacgcggcc
     7201 ggagagttgg gatcgacgtt gaaaagattc gtgatgatat cccagatttg gaacttgcca
     7261 aacgtttttt ttcatatgca gaagctgctg cgatcagccg tgttcatggc ctcaagaggg
     7321 ctgaattctt cctgaaaata tggacgagaa aggaagcact cctaaaagcc actggagaag
     7381 gaatcggggg tttagacaac agtattgatc tcattaataa atgcagtatt tcacacaatg
     7441 ggatattatg gcaggttcat gactttgcgg tcccctcgga atatacctca gcctttgtgg
     7501 tagagggggg agaatgtttc attaaaaaaa gagaggtatc tttccaaggt ggtttgaagg
     7561 ttttttactc ggttgggaga gacttcatca ataatctctc tcacgattca agtaaacata
     7621 cccggattct tcactcgcca cagtgaattc catttaaata cacaagtaaa catatttggg
     7681 gtgtttgtgg gaacgctcta ctttaatatg cgaaacgaga tattcgccag gttgatgtaa
     7741 agctggcacc aggaattaat ccagcacctg caaatgagac tctttcccac actatttaag
     7801 cattgaaaca aaccactctc aatcctgcat gaaagaccgg cgccgcattg acctgaaggc
     7861 catcgcccgc gaggcgatgg aaaagtatgg attcgaaccg gaattcccca aaacagtgat
     7921 aaaagaggtc aatgccatgt tacctgacct ctccgcacct gggcggcacg atatcaggga
     7981 catgagatcc ctcctctggt cttctataga caattacgat tcggaagacc tcgaccagct
     8041 ggagtactgc gaagctggca gtaggggaga gatcctggtc cgggtcgcca tcgcggacgt
     8101 ggaccacttt gtcacaaagg gctcgcatac cgatcggcac gctgcccaca acggcacgtc
     8161 cgtatacctg ggagttgaga cgttcccgat gcttcccgac cggctctcga aggggatctc
     8221 gtctcttctt cccggagagg accggtgcgc gatggtagtg gagtatacgg tccttacggg
     8281 cgggagcgtc cggaacggtg gaatctaccg cgcgctggtc aacaacaagg caaagctcgt
     8341 gtatgaggag gtcggcgact ggctcgaggg tgccgcccct gttcctgtct ctgtcagcag
     8401 tgtcccgggc ctcgaagagc agatccggct tcaggcggag gtgacccgcc ggttgaggag
     8461 ccgccgcatg caaaagggtg cccttgacct ggagaccctg gaggctgagc cggtcatgaa
     8521 aggcgaaacg gtgcattccc tcgtgatcca ggagcagaat gccgcccgcc agataatcga
     8581 ggagttcatg gtggctgcaa acggcacgat ggtgcacgtc ctcgggggtg cgaaggtccc
     8641 catgatccag cgggtggtca gagtcccaaa acactgggac gggatcatgg agactgctgc
     8701 agcataccgg tacaaattgc caaaacagcc ggactcaaaa gcgctcgcaa agttcctcga
     8761 caggcagagg gcggcagatc ccgtgcggtt ccccgacctc tccctgacga tcgtgaagct
     8821 gatggggccg ggtgaatatg tcccctttgt tcccggcgat acaccgatcg gacactttgc
     8881 gctggcagtg gtggattaca cgcactccac cgccccgaac cgccgctacg tggatatcat
     8941 aaaccagcgc ctcctgaaag cggtgcttga cggcgaggcc gtgccctact cgggccacga
     9001 acttggccgg ctcgcagagt ggctctcgga ccgcgagaag gcctcccaga aggcagagcg
     9061 gttcatgagg aaggttgcag ctgcgttact cctcgagaga cgtattggcg agacattcga
     9121 tgcgattgtg acaggtgccg cagaaaaagg gacgtacgtg cgtcttctcg atccccccgc
     9181 ggagggaaga gtggtggaag gggaacgcgg gctccgggtg ggcaacaagg tcacagtcag
     9241 gctgctctcg accgatcctc cacggggata tgtggatttt gcatgcgtga agaagcctcc
     9301 acggtaaagg cacaacgttc atcgccatcc ttcgcgaaac accaccccat ggcaggccag
     9361 gtcatcctcg taaccggttc ctcggatggg atagggaagg ctgctgcccg tgagctggcg
     9421 gcacgcgatg ccgaagtgat cgtccacggg agggaccccg ggaaggggag gcaggtgatc
     9481 agggaactga aaagtcaggt ctggaaccgg aacctcgacc tcgtcaccgc ggattttact
     9541 tcacttgagc aggtgaggag aatggcagcc gaggtctcgt cgcggtatac ctgcctggac
     9601 gttctggtga acaacgccgg gacctacgag aaacagagga gcctgactcc ggacggcatc
     9661 gagaccacgt ttgccgtgaa ctctctcgct ccgttcctcc tcacgcatga acttcttccc
     9721 ctgctccgga aaggccatct cccgcgggtg gtgaacgtcg catccatcgc acaccgggag
     9781 atcaggcagg tcgactggga gaacctccag ggagagaagc gatacgacgc cttcgaggca
     9841 tatgccctct cagcgctcgc acacgtgacc ctcacattcg gaatagcccg gagaatcgca
     9901 ggcagcggta tttcggtaaa ctgcctccat cccggaattg ttgccaccaa gatgcttcgt
     9961 gcaggattcc cggggatacg cgggaaagca ccctctgaag gggcgaaact cccggtgtac
    10021 cttgcgatat cacctgatgt cgaggggatc acaggagagt acttcgaaga gagcgttcaa
    10081 ccgacgctgc catctgacct ctcacggagc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn gggcacactg ggatctcagg attcccatcg tgatcgttgt cgagggctgg
    10261 aatgcgtcgg gcatcaccac ggtgatcagt gagctgatcc agtacctcga tccccgtgga
    10321 ttcgtgctcc attccatcgg gtcaccaaaa gatgaagagc gtgcacgccc cctcctgtgg
    10381 aggttctgga cgagaactcc tgcaagggga aggatggcca ttttcgcccg gagctggtat
    10441 tccaggtcgc ttgccgagca ggtatcgggt attgactgga aatcccgaat aaaacgcgcc
    10501 atctttgata tcaacacctt tgagcggcag cttaccgacg atggtgttgt catcatcaag
    10561 ttcttcctcc atatcagcaa ggaagagcag agaaagcggc tgaacgagag ggagaaggat
    10621 gcactcacct cttggatgat cacccagggc gactgggact ttcatgcgca ctatgatgac
    10681 tatctcccga ttatcgagga ctacctttct ggcaccgata cccgccactc accatggact
    10741 gtgataggtg caacggatgg caattacacg cggtttaagg tatattcaac agtgatcaga
    10801 acccttgaag aaaaacaaaa gatcctggag aagaaacggc aggaaaaatg tggacctgcg
    10861 ctggcaaaac cccccaggaa caaaataaaa cggagcgcag aagcaccaag gcagtatacc
    10921 agagcagaat atgaaatgat cctccaggag tgccaggaac aggtccatga tatccagtac
    10981 ctgctcttca agcgcaggat tccccttatc atcgtatatg aagggtggga cgccgcgggg
    11041 aaggggggaa acatcatgcg gctggtacgc gcgatgaacc cccgcggcta tgatgtggtt
    11101 cccatcgccc gcccggacca gaccgagctc gagcaccatt acctctggag gttctaccgg
    11161 aggttccccg cagcaggtca tatcaccatc tttgaccgga gttggtacgg aagggtactg
    11221 gtggagagga tagaggggtt ctgcacagag agtgagtgga aacgtgccta ccacgagatc
    11281 aacgagatgg aggaggcgta cgtggaaggt gggggagggt tgattaagtt ctggctcgag
    11341 gtgagcaggg aagagcagta caggcggttc atcgccaggc agaacgacgt tctcaaggaa
    11401 tggaagatga ccgaggagga ctggcgcaac cgggagaaat ggaaagaata cgagaatgcg
    11461 gtcgacgaga tgcttgcccg caccagcacc agtatcgcac catggaccgt gatcgagtca
    11521 gacgacaagt actatgcaag gctgaaagcg attcaaacgg tcatttcgta tggaagcgag
    11581 gcacttgaat gaccgtatca ttcgcacttt cgcgatagca ggagcatcgg aaaaggggtt
    11641 taccataaca caaagtttct taaaggcggc tgaaggaatt gagactatgc atgatattgt
    11701 cactaccagg aaaatggaga atggtgtcgc ctgctactat ggtgagagcg ggaaagagaa
    11761 attcgagagc ttcacctata gggagcttat tgacatgaag atcaacgcgc tagaccttct
    11821 ggatgatcca aggaattacg cagtcgacac ggaaaagcat acactcgtga tgaagaagta
    11881 ggtaagatcc acacttctct ttctcccctc tgcatggcga ctagagaatg aggatagtgg
    11941 ttgataaaaa accatcgtct cctttctgca agtggaaccc gtaaatttcc agatggaaga
    12001 aaatgaaagc agggcagggt tgtttgctcc atttatttgg attggaaaca atctgcaggt
    12061 tattcgcact ttccgaagaa caccaaatat acgcgtttca taccattgtc caggttaaag
    12121 aaagatgctc tttcatatga acaatcaggg aaaggtatta cctcatgctg cccataaaca
    12181 ggctaagcgc gagtccggta atgagagcga ccagtgcatc tgtggtaaag tatgagaggg
    12241 aaagcagctg atcaagtgga attcctcccc aaaaaaaacc aaggatgact gaaaggaccc
    12301 ctccgataca gagcgctgca gcgaggtgcc gaagtcccct ccagtaatca tggtaggtca
    12361 ggcggataaa caccacgctt gccaggaaga acgccannnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnnngtcc cggctatccc gaagataatg gtgtcagcag tcaatccctc
    12541 aatcgcaatt ttaaggacaa atatccgggc aaactcatag aaagccagaa caacgattat
    12601 aaggaggaat acctttttta agacgaaaac gaggagtgtg gtgaattttt gaatgatgta
    12661 gattgcgata taaagaagga agaggaatat cacaatggca aagacatttc ccctcaatac
    12721 ttcgtccgga agggtggata catactcagt ggcccttacc tggacatagg taattgggga
    12781 gagatcaacc attggcagtc accctggcat ctatcaccta caacggcctt gaatatatcc
    12841 gttcctgtta ttttcatcct gtttggcagg agatatctca agaaaacaat cactggtgtg
    12901 tcggttgtat gttgaaacac ccgaatcagt gatatatttg cgagatcgcc cagatggtgt
    12961 ctccgtgaca tgtcattaca ggaacccaac tgggtaattc ctccgccatt ggcttcgttc
    13021 ccggagtgtt cagttatcgc agcgaaattc tattaggatt actaaattgg atgttttttg
    13081 agggaaagta aaggtatctt ttcggtgtgg ggatacaaag gagggatatc ctccgggcgt
    13141 cagaacaccc agtagagaga gagaatatat acaagatatc cccctacaag cagggcaccc
    13201 caggctcttg tcacggtcat tttccctgaa aacagaggga ggattgccat ggaaaagagg
    13261 agcaatgggc caatcgtcac catctccggg ggagggatag ggatgaagag ggcgtttatc
    13321 cccatcacga agaggaggtt gaagatattg cttccaatga tattcccgat cgcgataccc
    13381 ggcctgttgc ggtatgccgc aaccacggtg gtggccagtt caggcagtga agtccccacc
    13441 gcaaccatag agagcccgat gactaccggg gggatggcaa agagggcggc gatctccact
    13501 gcaccggtca gcagcaggtg ggcgccgatg atcactgccg caagaccgcc gatggtaaag
    13561 aggagggaat aaggagagtc tttctcggca gctctctcca tcacatggcc tgatctccat
    13621 aaaatgacca cgagggctgc aaagagtgcc agcataacga tacccgagag gaggtcgagg
    13681 acgcttctcc agcacaggag agcaaaagcc accgtggcgc taagcatgag ggcggtgtgg
    13741 agcatgaatc gttgccgcga gagatgcacg cggaaggcgg gaggtgcaag tacggcatag
    13801 aggccgagta tgagtgcgat gttcgagatg ttgctcccga tgatgttccc ggttccgatc
    13861 ccgaaatttc ctgcccggaa ggcttcggag gtgaccacca gctctgggag agaggttccg
    13921 aatgccacga ctgtcagacc gataaacgtt gccgagatcc ccaatcggga agcgatcccc
    13981 tctgcacctt ccacagtgag gtcggctccc ttgaggagca ggacgagacc agccgaaaag
    14041 aggatgagcg ggatgatcat gtggcattca ccgttgtcgg actggatgcg tgatgcgtgg
    14101 agtcatccat gatttctcgt ggttgccggc actgtctttt cgaaaaccca tccccaggac
    14161 cacatcgaat ctccgcatca tcctgtgctt atccgttccc tctggaattg aatatacgag
    14221 taaaccacgg tgtaccctgc cacaagcaaa accggaacaa ggcagaacca cagcgcaaag
    14281 gacccgaaga agactcccac aatggatacg atgccggcga tcttgaagag cctgccaccc
    14341 atagcgtgcg tctttttcca gaccttttca ctgctcatgg tccagggagt acggatccct
    14401 acgaaccagt tcggctctgc atggtcgagc aggaacccta tataaatgaa gagcgccccg
    14461 aaaaggggag ggatgacgat attgggactt atctcgatcc caatgcccca gaggagtatc
    14521 tgcacctgga tgatcgcaaa aaacagggca aagacgagaa tgaatccttc gtagtagggc
    14581 tggaacttcc tgtaattctc acgcaagggg tcaatccgcg gtatcaggaa gaagagtgcc
    14641 gtgagaggga tcatgatcaa gggaactatc gcagcacccc agagtctcgg gaggtacccg
    14701 tccacctcgc cggcggcgtt ccagtgcgat gggacctgat cgggaagggc cgggtagact
    14761 atggctgtga tcgccagggt caggaggagc acagctacaa cgcctaagcg gaccagggtc
    14821 atctgatcag tacaatggtg cggagggaga aggatgttgt cttttccacc cggttggcat
    14881 ttggggggat catcgttgtt ccgggtatct tatgcgttcg gggaataaaa tattctccaa
    14941 tgacaggttt caggcgttcg cacctccgcc tgttaggcat ggcagttctt gccggtgctg
    15001 ggtttcgtct tggcattccg ggtcttgccg aagtgatcga aggcatctgg agccatgcag
    15061 aaccggcatc gagagccttg ctccctcttg aactccaggg gatgataggg atatttggca
    15121 tccttgtgct gggtatcgtg atctacctca attacgagat atttatgaag ggggcaaaga
    15181 gaggaaggag aggactttta attaccgtat gcgggttttg tgcgggtatt ctgctggcaa
    15241 acatcgtcat tgcagcacta ttccaatgat cccctgattt cgggagaaat tttttacctg
    15301 cccggacaat gaatgaaggg agagagaaaa atgctgttga atattgagat tttgatatac
    15361 atatctcatg agggccggat taagaatgct ggatcataac cgggagaagt tcgggatcat
    15421 tcttctcaat tggaacgccg aggagctcac tactcgctgt gtcagggcat tgcaggaatg
    15481 gcacaagctg aggccccata tttatgtcgt tgataacgga tcaagtggaa ctggcttgta
    15541 ttttgaggat agccgggatg tgccgatatc tttgataaag tcaccgaaga atcggggata
    15601 cgccgggggc aacaatctgg gaataagaaa ggcaatcgaa gatggttgtg agtatctcgt
    15661 ccttcttaat tctgatgcat acatatccga aaataatatg gaaatacttc tggaccaatt
    15721 caggagatac gatgatattg gttgtttggg cccaggtatc tgcgaaggag acctgttatt
    15781 ccttggtggc agggatatgg ggttgcatcc caatacaagg aataccttgt ctcaggagac
    15841 atccatgaag ggaatcatgt atgtcgatta tgttcccggc atggtttttc tgaccacgaa
    15901 ggacgttatc aaacgcatcg ggtttcttga cgaacaattc tttttcagtg gcgaaatcgc
    15961 tgacttctgc aaaagggtgc tgaaagcggg gtatcggtgc gcagtctgcc cggatgtcag
    16021 ggctaatcac gtggtagtcg aagacaatcc cctccgaagc tcactgtatg cgtattattc
    16081 ttttagaaac cgatttttat tcgtcaggaa gcaccattca aatatccgat ggatccttga
    16141 gccattctgg atactttggg gcatgcacaa gtacgtccgg ggagtgattt tgggcctcga
    16201 taacgaatct gcagcatacc ggatggcgat tatggacgga gtgaggggtg tgtttgggga
    16261 ccgaaatgac ctcttttccg tatagtgcat cgatcatcat tccaaactgg aatggcatga
    16321 attggcttcc aggatgcctg gctgccctga aggaccagac cgtgcaggac tttgagataa
    16381 tagtcgttga taatggatca agtgatggtt cggttcctta tatccagagt aggtatcctg
    16441 ctgtacggat cattacattc aggaagaatt gcggtttttc caaagcagtg aatgagggga
    16501 taaaaaaagc ttcaggaaaa tatattgtcc ttctcaacaa cgataccctc cccgagaaag
    16561 aatggcttat cagtcttctc aatgagataa gggctgctcc gatagaggta gggtcccttg
    16621 cttcactcat gataaggatg gataatccat ccctcacaga tgatgccggc gattacctcg
    16681 attggtatgg actcgcgttc aagaggggca acggagagcc aatctttcaa tggatagaga
    16741 acgagcctgt attctctgcg tgtgcgggag cagtattata cagacgtgaa tttctcgaga
    16801 gaacgggatt gttcgatgaa aaattttggt cataccttga ggacgtcgat ctcggcctca
    16861 ggggaaacct cctgggattt acctgtattt tcgttccctc ggcaagggta cttcataaga
    16921 gccatggatc agcaatgcct tcaggggcat atgtatatca tgtaacaaag aacagggtga
    16981 tgctattgtt aaaaaatatc ccttcgattt tactcgcaca gaatgcccga acacttattt
    17041 ccggccagat tctgacactc cggatcaaca aaaatcccag ttcatccatt cgggcctatt
    17101 tgtatatcgt gtttctcctt ccaaagatcc ttcatgacag gcgttgtttt ctcccacgtg
    17161 tggtcctttc gattgatgcc ataaacactc tcttgcgaaa aacggggaac caatgaaagc
    17221 gaatcttcaa aataatgagg gatgctctgg atgatgtatc ttgctttttt tcttgggcta
    17281 atgataccaa tattcgaata gtataaggtg aattgtgtaa atactatgat tggcaggtct
    17341 ttttggagag ttaggtatgg agaaagatat cgagattacc gtcatcattc cgacgttgaa
    17401 tgaagaaatc tccatacacc gttgcatctc caaaatattg gcggtgttcc gcgagcatga
    17461 cctcgaaggg gagatcattg tttccgattc ctcgaccgac cgtaccccgg agattgcagc
    17521 ctcccttggt gcaatggtcg ttcgccccga acagaaaggg tatggcgcag cctatcttgc
    17581 ggcttttccc cacgcccggg gaaaatatgt cgtaatcggc gatggcgatg acacctacga
    17641 tttttcccag atcccaaaat tggtcgaacc actgaagcac ggtgctgaca tggtgatagg
    17701 gtcgcgattc aatggcaaga ttcgtccagg ggcaatgaca ccgctgcacc agtatatcgg
    17761 caatccaatc ctcacctgga tggtgaacgt actctttcat gcggatttct ccgatgtgca
    17821 cagcggtttc cgtgcaatca ggagggaggc actggaacgg cttgatctcc ggagtcccgg
    17881 gatggagttt gcatccgaga tgatggtcag agcaaggcag aaaaacctgg tgatagaaga
    17941 ggttccgatc acctattatc caaggcaggc cccgtccaag ctgcacagtt ttgccgatgg
    18001 ctggcgacac gtccggttcg tgctcctttt gaatcccctt ccattcattg ccatacccgg
    18061 tgcgatcttc gcgttcgtcg gatttttatt gatgattctt ttcgcattgt ggggccaggt
    18121 cacgacgtcg catcttcact cgtttattct tggggcattt ttactgtgtg gcggagtcca
    18181 gatcgtggtc attggtgtcc tgataaagat atattctgtg acacaccggt acgaggagaa
    18241 ggcaggaatt atcgtatcga taatgaatta ccatagcctg gagaggttcc tgctggtcgg
    18301 aggactcatc ctctcgttgg gttttttatc gggattgagt gtgataattg cctggattca
    18361 gtcaggattt ggccaaatcg ccgagattgt gaacgcggtc gcagccctct gcctgggcac
    18421 aataggcctc cagatcatct tcatggccat ttttaccagc atgatgctgc ttcgtgaaga
    18481 gaacggggat accaagaatc ctgcatgaaa attgccttcg tgtatgacat ggtctaccca
    18541 ttcaggattg gcggggtaga gaaacgggtc tgggagatct cacgaaggct ttcggcgagg
    18601 ggccacgagg tacacgtttt tgggttgaaa atgtgggagg ggccgccgga cacttttcgg
    18661 gaaggagtgt atctgcatgg agtctgtcgc ccgatgccgt tccatacagg tgagagcggg
    18721 agaaggacta tctttcctgc gctctggctc tcattgttcc tgttcctccc cctcctgcgc
    18781 aaaggaagat tcgatgtgat cgactgccag aacttcccgt attttccctg ttttcctgcc
    18841 ggagtggtcg cagcactccg gaagtctctc ttccttgtca cctggcacga ggtatggggc
    18901 aggtactggt tctattacat ggggagggcg ggatcggtcg gaattttcat tgagaggctc
    18961 gtgaaaaatc tcacccggtg tcatattgcg gtctccccca ccacgaaggc gatgctgctc
    19021 gatcttggag ttgaggagcc ggttgccgtt gttccgaacg gggtggactt cgagaggatc
    19081 cggaaggtcc ctccctcttc tgaacaatcg gacatcatct gtatcgccag gctgattcca
    19141 gacaagcacg ttgatgtgtt gattggtgca gtcgcgctgt tgaaggcatc tatccccgag
    19201 atttgctgca ctattgtggg aagggggccg gaagaagaga ggttgaacgc gcaggtttcc
    19261 ctgtcagggc tggataagaa tatcaggttc accggttttg tcaaggacca ggacgatatt
    19321 ctcgctctcc tgaaagcgtc cagggcatgt gtgctccctt caacgaggga gggattcggg
    19381 atggttgcgc ttgaggcgct tgcatgcggg atcccggtcg taacggccga tcatccagga
    19441 aatgcgatac gcgaccttgc ggagatgggg ggggtcaggg cagtgccgct tactgcacag
    19501 gattttgcgg ttgcaataca ggaaacactc cagtctcctt tatctacctt tgttccttct
    19561 cttgaaggcg catgggactg ggatgcagtt accgaacagt ggctcgggat ggccgaagaa
    19621 tttttacgga tatcgaaaga acacgcggca aaaggttcat tgtgagaacc tgcaaagtgt
    19681 atgtttgaga aaagggctcc ggattatgag aataagcatt atcggcgcag ggtatgtcgg
    19741 gcttgttact ggtgcctgtt ttgcagagct cggccatgaa gttcacctgg tagagatcga
    19801 cagggaaaaa gcaggtgtga tcgacagagg cattgcgcct ttctatgaac ctggcctgga
    19861 ggccctttta aaaaaacata ttcaccgaac ccttgcagtt tcatgtgaat accaggaaat
    19921 cactggatca gactgcacgt tcatctgcgt cggaactcct ccctcacctg atggtagcgc
    19981 cgacctccgt tttatccgta gtgccgctca ttccattggc actgctctga aaggagggag
    20041 agatccgcac accgtggtag tcaagagcac ggtgccaccc ggaaccacca ggtctatcgt
    20101 ggcgcctgcg gtaatggagg catctggaag gcatgacggc ccgataacct ttgccatgaa
    20161 tcccgaattc ttgcgggagg gaagggcagt cgaggacttc ttccatccag atcgcatcgt
    20221 aataggatgc agtgaggata ttgcgtgcag gaaggtagcg gctctttacg acggtatcga
    20281 tgcccctctg gtgacggcag gacttgatgc tgccgagatg atcaagtatg tctcaaatgc
    20341 atttcttgcc gcaaagatct cgttctcaaa cgaggtcggg aacatatgca agaaactcgg
    20401 gatcgacgtg tatgaagtga tgaagggtgt cggtctcgat acccgtatct cggcgcattt
    20461 cctgaatgcg ggggcaggct ttgggggaag ctgcttcccg aaggatgtct ctgccctgat
    20521 tagcctggca gagtcaatgg gagaaaatcc gagaatcctc aaatcggtaa tggaggtaaa
    20581 tgacagccag gctcttcgca tggttacgat gcttgaggcc cgcatcggga acatttgtgg
    20641 gaagcaggtt gcagtgcttg gccttgcgtt caaggacgat accgatgatg tgcgggactc
    20701 gagggctatc ccggtcattc aatcgctggt ggacaggggt gcgctggtgc gggcatttga
    20761 tcccattgcg aatgaggcga tgtccaggat ttttcctggc atcaactact atggatctgc
    20821 agaggaggcg cttaccagtg ctgatgcttg ccttgtaatg actgagtggc cggagttctc
    20881 acagctcgac cttgagttcg atcggatgaa atcccgggtc atcattgagg gaaggagaat
    20941 cctctcctgc gggggaaagg aggggatatg ctggtgaggg ttcagaggtg cagtggtgca
    21001 atgtgatggt ggggagaaaa gaaatttgaa agttgatcat actttttgct gcttattttc
    21061 tgattcagtg attaggaaaa aggaattaat gccacgttct ttgtgtaatg cggtaaggga
    21121 atttatagag aggtcttctc catgaaacat caacatgtct cgaaagtggt gatccccgcg
    21181 gcggggctcg gtacgcgctt cctcccgatg accaaggccc agccaaaaga gatgctccct
    21241 gtggtcgaca aacctgttat ccagtacgtg gtggaggagg cagtggcatc aggcatcgac
    21301 gatatcctga ttataacggg gcggggaaag cgtgcgatcg aggaccattt cgaccgttcg
    21361 tttgagctgg agcaccggct caaggagtcg ggaaacctgg agcttcactc cgagactgaa
    21421 gaactttctc agctcgctga aatccactat atcaggcaga aggagccgaa aggtcttggc
    21481 catgctattc tctgcgccga gaggcactgc aacggagagc cgtttgccgt gctgctcggg
    21541 gataccatca ccgtgcccta tcccggagag aaaccgtgca cggcgcagat ggtgaaggcg
    21601 tttgctgaat ggaatagttc gattatcgca atcgaggagg tgcgaaaaga gaaaatacag
    21661 gactacggga tcatcgacgg gacagaagtc aggagcgggc tctacaggat agatgcaatc
    21721 gtggagaaac cgaaacctgc cgacgcaccc tccaaccttg cctccatcgg ccgctatgtc
    21781 cttaccccgg atatattcga tctcctgcac gggactcgac ctgggtatgg cggggagatc
    21841 cagctgactg atgccatcca gcaactcccc acaaagatgg gcctggtgac acgatgcacg
    21901 cgatacgaca tcggggacaa gctcgggtgg ctgaaagcaa acctcgaatt ctgcctccag
    21961 cgggaagagt tcaatgatga agtactcggg tttctcactt cccttcaaac gaagaataga
    22021 gtcaaacatc cggagaaata gcaccgattg acaccatact gcatgaatcc atttttttat
    22081 cgtccgaaac gagttatact ggtgtcttca agggggcctt tatatcaatt tgtcctggag
    22141 caccccgggg tattttatgc aagagtgtgt ctctattcgt atggttattc ttgatgtcgg
    22201 acgtaagtga atgcggggaa cctcctggtt gtacttacga tttgccattc caggaatata
    22261 cggcaccagt gaatcttttg caggaatata tgaggattcc cggtaattcg caacctctat
    22321 tatgaaactc acatttgcga tctccatttt gtatgcgcgt gtcttgaaga gaaccatgtg
    22381 gttttttatt catcctgccg caagagaaat cattttttag tttttccttg tatattatct
    22441 catgactggc cagagtgaca cccatccatc ccttccaggt actgccttcc cccggttctg
    22501 tggccaggta attgcctatc ttatcgacgc gcctgtacat ttttcatcca gaaattcata
    22561 tccccccttc aatttttgga cagctgcaca gcgtttcacg ggatatgcag aactgagaga
    22621 gataacaagg catcaggcga ggatggtgta gctatggagg gtaaggccgc gttcattata
    22681 ctctgtgtcc ttcttacgac cggaatttcc gggacactgg cacagaataa tacttctctt
    22741 gtatttttgg aaaacgagtc aaataattct cttcagatcg atccggttat tctcaattct
    22801 actgtttttc atggctacga taccgggagc gaggatgacg aagacgtagg gaatccctac
    22861 cctgtggata tgtccgagtc ctcaaccgag gaccaggggg gcccgggcgg gattgttaca
    22921 ggagaagcgg ttgaagggga agggtcaagc aacggtaacc tgacaaatgg aaaagaaaag
    22981 aaggataagg aaaaagccca atcctccgag gacgaaatca tcatcaggtt cagggttgag
    23041 cccggcaagg agatgagcgc cggagcggcc gagacggctc acaaggatgc cagcacaaag
    23101 gcaaagaagg actccaaggt gaaggaggac ttcagagtaa agaatgactt cgacaaaaag
    23161 gggctccctg gcacccaggt cgtgaaggtg cctcctgggc tttcagtcga tgaagcgatc
    23221 gctctgtatt cggagaatcc cgatgttctc tctgcagaac caaatcatcg tattgaactc
    23281 atgagtatcc ccgatgatcc gggcttctcc ttccagtggg gcctccttaa tgccggcaat
    23341 cctggtgctg atatccgggc agtttctgca tggaacattt ccaccgggag ttcaggagtt
    23401 ttaattgcca ttcctgatac cggggtagac atatcccacc ctgatttaac gtcaaacata
    23461 tggtccaatt cccgggagat tcccggcaac ggactcgacg atgatgggaa cgggtatatt
    23521 aatgatgtga acggctggga ctttgtcaac aatgattcct ctccggtgga cgacaatgga
    23581 cacgggacgc acgttgccgg catcgcaggc gcagtcggca acaatggcct cgggatttcc
    23641 ggagtgatgt ggaacacgcg gctccttccc ctgaaggtaa tcggggcttc gggatatggt
    23701 tacgagagcg atgcgattga ggcagttctg tatgcacaac aagcgggagc gcaggtcatc
    23761 tccatctcct ggggcagtta tggagaaagc caggcgctga aggatgccat agcaggatat
    23821 ccaggcctcg tggtctgcgc tgcgggtaac agtgcccagg ataatgacct gtatccgctc
    23881 tatccggcat catacccgtc gaagaacatc atctctgtta ctgcgacaga cgagcaggat
    23941 agtctggcac catttgcgaa ttatggcgct gtatcagtgg atatcgctgc tcccggagtc
    24001 gggatctaca gcacagtccc tggatccgga tatgatatgc gatccgggac atccatggct
    24061 gcaccatatg tagcaggggt ggctgggctc gtactgtctg tgaatggaga tctggatggc
    24121 gcgcaactcg cggatatcct cattgcaagt gctgacagat tgagttccct ggaagggagg
    24181 atctcgtctt ctgggcgggt caatgcggag tcggcgattg ttcttgcgac cgggcagacg
    24241 aacattccac cgacgccctc tcccacgatc acggtgacac cgacgcttac acccgtgatg
    24301 ccggaggggg aaccgcagct tgctccgttg aatccggaat ttgtggcata tatccagtct
    24361 catacggcaa atacgtcggt gactaccgat gaaggttttg ggcttgggta tatccccccc
    24421 cctcttgata tgacatggct ggagggacag gttctacaca tttccgacaa tatgattctc
    24481 tcccttccgc catcatatag cctcaaaact ctcggctgcg tgacatcggt caagtcccag
    24541 ggcagctgtg gctcatgctg ggcatttgcg acatatggat cggcggaatc agttctgatg
    24601 cctgaagaag catgggactt ttccgaagac aacctgaaaa atttacatga gtttgattat
    24661 ggtccatgtt caggtgggca gtattacatg tccactgcat atctggttcg ttggagcggc
    24721 cctctcacag aggcagcaga tccttacagt gattccccaa caagctattc tcctacagga
    24781 ttacttcctg taaagcatgt gcagaatgtg atatttctcc ccagccgtgg gactgccctg
    24841 gataacgata atataaagaa tgctatcatg acccatggcg gcgtaagtac tacattctac
    24901 tgggcatccg gttcatataa ttcaaacacg aaagcgtatt tttcgacgta ttcaggagaa
    24961 aaccatggtg taaccatcgt tggctgggat gatagctata gtaaatctaa tttcctcacg
    25021 tccccagcag gggatggtgc attccttgcg aaaaacagct ggggaccggg gtgggggaat
    25081 gaaggatact tctggatatc ctattatgat acaagccttg gtaagagttt gaatgcggtg
    25141 tttctatcgg agcctgcgga aaattatgat tatatctacc agtatgaccc gctcggatgg
    25201 gtatcatcag tgggatatgg gacgaataca gcatggggtg cgaataaatt cacttcagcc
    25261 agagacgagc aactcgaagc agtcggattc tacactgcat ccccgaatac ggcatatgaa
    25321 gtctatatcc atagaaatcc aactgatggt ccactcagta acacaggagc agtgtcctcc
    25381 aaaactggta taatcgatgt gccgggatat cacactatcc cactttccaa tccagtttcg
    25441 ttaaactcag gcgatacttt ttccattgtc gtgaagataa ccacccctgg gtatgcttat
    25501 ccagttcctt ttgagtatcc taccggggga tattcaagcc aggcgacgtc taaagcaggg
    25561 gagagttaca tcagtccttc cggggcatct gggacatggg tggatcttac cgactggcgc
    25621 tcaaattcga atgtgtgcat taaggggtat acaactacat ggaagccccc tgttgcgaat
    25681 ttcacagcga cacctgttac agcgatagtg ccagctcgca tacaattcaa tgatacatcg
    25741 acgaatgtac ccacatcctg gcaatggaaa tttggggacg ggaacacgtc gagcgagcgg
    25801 aacccggtgc acacatatga gggagttggg acgtacgacg tgaatctaac ggtgtggaat
    25861 gcgtatgggt cggacagcgt caccaagacg gattacatca cggttgtaga agctccggtg
    25921 tttctggaag gatggacata ccggaagctg gtgacgatcg cgggttcgcc ggatggtgtg
    25981 ctgaccgact accagatgcg gttcgtggtt caccagggcg aggggataga cagcggagag
    26041 gatgtctatg ttggcacgac tgtgaaggag gactattcgg atttgaggtt cacgacgata
    26101 gggaatgcac cgatgccgta ctggattgag gcatcggatg caggttctgc ggtggtgtgg
    26161 gtgaaagttc cggaaattcc ggtgacaggg acgcaggtgt atgtgtatta tgggaatcct
    26221 ggtgcggagg cagtgagtga tggagaagcg acatttattc tctatgacga tttctcgggg
    26281 tcttcactgg atacaagtaa atggaccaat atgttaggga gtggtgtgac tgtatctggc
    26341 ggagtcctga catgtgcagc atcgacaagc gcatcatatg gagttgaatc aaaaaatact
    26401 gatttgggtg taaatatatc aataagagca aaaatgtatc cgttccatcc aaaatcaacg
    26461 acacttcttg aaggcttata tgcgcgacat ggtcccacta cgccttacca gaatgcactc
    26521 tatttttcgc atgttgtttc aagttattct ggaaagtatc tctcacgaaa cggtgggagt
    26581 gtaacaactg aatcggttac aggattgggt ggtgcgggat ggtatattct cgaagggagg
    26641 atggactcgt catcaatcaa gtggagcctc aacgatgcga atcctgttac aatatcaact
    26701 aactactatc caacaagtag cgcattgaga attgtgtcat atgcatcagg agcaaaaata
    26761 agcacggatt ggatatttgt tcgtaaatac tcagcaacag aaccttcggt tattgattgg
    26821 acacaacagg aacaggccga aattgcaccg gtcgcagcat attgcagtaa tgtaaatgaa
    26881 ggttcgaaac cactgacggt tcaactcacc gatctctctt tattccaacc cacattctgg
    26941 cagtgggatt ttggggacgg gaacacgtcg agcgagcgga acccggtgca cacatatgag
    27001 ggagttggga cgtacgacgt gaatctaacg gtgtggaatg cgtatgggtc ggacagcgtc
    27061 accaagacgg attacatcac ggttgtagaa gctccggtgt ttctggaagg atggacatac
    27121 cggaagctgg tgacgatcgc gggttcgccg gatggtgtgc tgaccgacta ccagatgcgg
    27181 ttcgtggttc accagggcga ggggatagac agcggagagg atgtctatgt tggcacgact
    27241 gtgaaggagg actattcgga tttgaggttc acgacgatag ggaatgcacc gatgccgtac
    27301 tggattgagg catcggatgc aggttctgcg gtggtgtggg tgaaagttcc ggaaattccg
    27361 gtgacaggga cgcaggtgta tgtgtattat gggaatcctg gtgcggaggc agtgagtgat
    27421 ggagaagcga catttattct ctatgacgat ttctcggggt cttcactgga tacaagtaaa
    27481 tggaccaata tgttagggag tggtgtgact gtatctggcg gagtcctgac atgtgcagca
    27541 tcgacaagcg catcatatgg agttgaatca aaaaatactg atttgggtgt aaatatatca
    27601 ataagagcaa aaatgtatcc gttccatcca aaatcaacga cacttcttga aggcttatat
    27661 gcgcgacatg gtcccactac gccttaccag aatgcactct atttttcgca tgttgtttca
    27721 agttattctg gaaagtatct ctcacgaaac ggtgggagtg taacaactga atcggttaca
    27781 ggattgggtg gtgcgggatg gtatattctc gaagggagga tggactcgtc atcaatcaag
    27841 tggagcctca acgatgcgaa tcctgttaca atatcaacta actactatcc aacaagtagc
    27901 gcattgagaa ttgtgtcata tgcatcagga gcaaaaataa gcacggattg gatatttgtt
    27961 cgtaaatact cagcaacaga accttcggtt attgatttgc cttcgtttaa tattcaacac
    28021 cttaaaataa tcgaaagatg aacaaaaaaa agataaaaaa attcattgta tattgaatga
    28081 aaataaaaaa atattaaggt tttattgaaa tgaatttccc tggtaatgag aggttattta
    28141 ttggaaacat gaaagattgc tatttgggat attccaatta tcaatcaatt cgaaaacatt
    28201 gtgcttctgg tgggataatt tcaaattttt taattttttt gctggattgt aaaataattg
    28261 acggtgcttt agtatgtaga tctaaagtaa ttgataataa aatctcgtat gaggtgaaaa
    28321 ttgttacatg ttccgaagat attttagatt atgcatcgag tgtatatttt aatattcctt
    28381 tattagaata tattcctatg attaaaaaat ttgatgggaa aattgctttt gtaggtcttc
    28441 cctgtcagat ccgaatcctt cgaaatctta tgaagaatga tataattctt cgtgaaaaag
    28501 tttttttaat aattggacta ttttgtgggc ataattcaag gaggaatctt ctcattgatg
    28561 ttttaaatca aaaaggaatt cgagaatttg acgtgaaaga aataatattt cgtcaggggc
    28621 tatggagagg gaatatgcag gtaaccctca aggacgataa aaaaatatct tttccatttc
    28681 aggattttag tatttatcaa aatttacatt tcgattcatt aaaaaaatgc ttattctgcg
    28741 ttgatcatac ggcggaagat gcagatattt catgtggcga tgcttggctt tcttatctca
    28801 gaaatgatcc aataaaacat tcacttataa ttactaggac agatttttca aattatttta
    28861 ttcatgaaat gagacgtaag ggagatattt ttttaatgca aattgaacca gaaataatct
    28921 ataaagctca aaaaagatct ctaatatctc ataaaagtat agaagcacgg tcaaaaatcg
    28981 gaaaaatttt tgggtattct atcacatacc cttcagagta ttacaatact gctagatgga
    29041 atgattatct cgcctcattc attttaataa taaatattca aatttcatcg aatgaaaagt
    29101 ttagaaagta tatatttttt attcctaaga ttatcttata cccatacttg tttttattta
    29161 agtcattaac aaacttttag caatctcaca attaattctt gcagaattat caacatttgg
    29221 aagaaatttt tcaaattttt ctgtaactgt tcagggtcaa ccgcaagatc ctagtacaat
    29281 gacgtcatag taacaa
//
LOCUS       DAOQYR010000019        26043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_2183_length_26043_cov_8.314222, whole genome shotgun sequence.
ACCESSION   DAOQYR010000019 DAOQYR010000000
VERSION     DAOQYR010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 26043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26043
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2183_length_26043_cov_8.314222"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PKM87_03965"
     CDS             complement(<1..614)
                     /locus_tag="PKM87_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNQ29256.1"
                     /translation="MDPLIARIRKELSEHADPQIQKNSQRFFKEEIRCYGMKTARVVA
                     IAKQYWKEVKPRDKGEIFALCEDLYRSGYMEESFIVSEWAQRLSDNYEREDFSVFRHW
                     IGTYITNWASCDGFCNHTMGAFIEQYPDTIDELKRWTRSENRWMRRAAAVSLIIPAKH
                     GKFLEESLAIADLLLTDGDDLVQKGYGWLLKEASRMHTGEVFSFV"
     gene            836..1228
                     /locus_tag="PKM87_03970"
     CDS             836..1228
                     /locus_tag="PKM87_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29257.1"
                     /translation="MSTLRFLLEHPIRARKVKEAVGSKCELCGKISNTDDLEVHTFID
                     PGKEQEMPAEELECFLLVLCQQCHEDLHNLVAGSRAQEILVRQREESVRKKIRAILGY
                     SPRPYNPPDSDVEGAYKDACASKFGNLI"
     gene            1422..2348
                     /locus_tag="PKM87_03975"
     CDS             1422..2348
                     /locus_tag="PKM87_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNQ29258.1"
                     /translation="MKQQILISGALILLSALLGVAHSLLDDLVVTNLFLTSLVLAASY
                     LVFSVLLGQLVVRRIQDQKTRYTARKVLSIISIVVILVATLRIWITDTSSLIVSYGII
                     GAALAFAVQDVFKNFVGGILIIASGYFRVGDRISIGDHVGDVMDIGIMNTTMMEIRGW
                     VKGDQPSGRLVVVPNAQVINHVFFNYTRDHSFVWDEISIPLTYESDWRKARDIILSIL
                     KRETDTMTEQAEIEIERIGEKYFLPRKVVEPSIYLSLTDNWILLEARYVADTRTRRIL
                     RSRLSELILAALETEETITVASETMTVTTVQK"
     gene            2640..3134
                     /locus_tag="PKM87_03980"
     CDS             2640..3134
                     /locus_tag="PKM87_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HNQ29259.1"
                     /translation="MSDFTLLPLAGSDGAEVIDLFNYYVRSSFAAFPEKPVPPEFFDR
                     LTQAAEGYPAVAARDRDGALAGFGMLRPFNPMPAFAHTAEVTYFVRPDVTGRGIGTAM
                     LRHLTAEGKKRGISVILAHISSRNEGSIRFHEKQGFFEVGRFRGAGKKRGLPFDSVWM
                     EKLL"
     gene            3345..3794
                     /locus_tag="PKM87_03985"
     CDS             3345..3794
                     /locus_tag="PKM87_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HNQ29260.1"
                     /translation="MVFICGLLAGLGLSPLLFSQKPHTEKTVIVSSNAPEPVGPYHQA
                     VRYGDLVFLSGQIGLYPGNGSLAGTTEEQTVQAMENLNAILQESQLDFSDVVQARIYF
                     INASDWDTINKVYGRYFEGAYPARAAVKVAGLPKDAKVEIEMIARVC"
     gene            3926..4972
                     /locus_tag="PKM87_03990"
     CDS             3926..4972
                     /locus_tag="PKM87_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNQ29261.1"
                     /translation="MLPEEWPSGSRALVLAGFFFLVIIGLKFCAYVVTIIVISLILTL
                     IACPAMDRMRKKGIPDIASVTILTLVAIAAVLVLIGISLYSFGVLMHDLPLYQAELNQ
                     RMAQISGMLAGHGLPTGAIPPSPDLGSLVGLILSSVMNLSDLLLYLFFIGVATFFMML
                     EAPHLADRLTRLPGVPPEKMARLSRMSGYMIDFVVVRTETNLIHGILFGGSLYAMGVH
                     AAMLWGVLTFVLAYIPFIGLIIAAIPAIFFAWLQFGLWGAAAVIAIVCILNLVVENPV
                     FSYFAARKFEIPALIVILSVIFWGWLLGIAGMVFAVPLTLMVLITIQYSDDLAWVNTL
                     LGVDRIFGESTENT"
     gene            complement(5187..5645)
                     /locus_tag="PKM87_03995"
     CDS             complement(5187..5645)
                     /locus_tag="PKM87_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNQ29262.1"
                     /translation="MRVIGGCLAGPVDEMKFYPPKDPARFVPKVALDRLRSYILASYL
                     SGNPGEVRLSYGQYGKPIVDTPSCPYTFSTSFSGKNWMLDISHENPVGVDIEQIKEIP
                     DLISNTHQLFHTEQLNLISLLPPIEQTQAFFNLWTLKEAYLKATGKGWSG"
     gene            complement(5891..6184)
                     /locus_tag="PKM87_04000"
     CDS             complement(5891..6184)
                     /locus_tag="PKM87_04000"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HNQ29263.1"
                     /translation="MGSSETVSGRGVLRKKDQIVLREEFDDWALLFDPDTGKVCGLNP
                     VGVRVWKMIDGKRTVHDISMMVQDSYPDAPDSVLEDVTAFITEITEKGYATVL"
     gene            6254..6952
                     /locus_tag="PKM87_04005"
     CDS             6254..6952
                     /locus_tag="PKM87_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HNQ29264.1"
                     /translation="MENDRQIRYVGNSMSPWLLQNDILDWEPINERLPVPGDVIIFRD
                     PANTGCLIIHRVVSKYPDGSYCTRGDNTLSNDGHPVMLSSIMGVVTRGIREERPLDVS
                     MGFAGLMHHQYAQQRVKLLSIFEHFFTRPYRKISQKKVMCRIVPQRYRQRLVIVKTHE
                     GYNLQVYLRKRLVGWKGEKDQDWTILPPYRLFIDWGNLPDCPVKFLNGVYTKTQYSVT
                     DDNQPTPQEGVRGD"
     gene            7124..7981
                     /gene="scmC"
                     /locus_tag="PKM87_04010"
     CDS             7124..7981
                     /gene="scmC"
                     /locus_tag="PKM87_04010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SynChlorMet cassette protein ScmC"
                     /protein_id="HNQ29265.1"
                     /translation="MTGPSYELVIRNGQRWALEASPDLEDWLQHFARIMMLREVDGRR
                     KSPSWRFCFQDSDVPLFHGGGWVTSRSRWMSGYAKTDGTDLVTEIIPFDRGDPKEDIV
                     RMMLAIQTLYLAIWPFGGIPVHAALLERDGEAILIAAPGGTGKSTCASRVPPPWQTLC
                     DDTVLLIKAGGVYYAHPLPTWSDFLIRDLYDRRWDVMYAVPVTGIWFLEHGEQDQAVP
                     LGKGQAASLLYQSAVQVSSICLRKENNDDIYAEWHMGLFHRSCDVVSHLSCGILRATL
                     DGSFWECLS"
     gene            8271..9380
                     /gene="scmE"
                     /locus_tag="PKM87_04015"
     CDS             8271..9380
                     /gene="scmE"
                     /locus_tag="PKM87_04015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SynChlorMet cassette radical SAM/SPASM protein
                     ScmE"
                     /protein_id="HNQ29266.1"
                     /translation="MKSPKSMDLDITSRCNLKCKYCFQRTSPTDTKTELPTEEWMAFC
                     GELGDCAVMSVCIGGGEPFIRDDLLEIIDAVIENRMRFSILSNGGLIRPEHAEYIAST
                     GRCDYVQISVDGSCPETHDSLRGSGSFEAAMRGVRTLQDAGVPITSRITIHRYNLDDL
                     ENTVHLLLDEIGMPSISTNSTCYLGVARKNPDEVALTPAEFCDAMERSLIIAEQYPRR
                     ISAADGPLALGRTFQEMEKARAEGRAIPGRGYLTGCGCMWSKLAVRPDGVIVPCNMLA
                     HIELGRMNRDLLLRIWQDHPELNRLRNRYTIPLSTFSECADCPWQLTCSGNCPASSYT
                     RTGNVFAPSPDGCLKRFLEHGGRIIRFPQSTWKRA"
     gene            9499..10578
                     /gene="scmF"
                     /locus_tag="PKM87_04020"
     CDS             9499..10578
                     /gene="scmF"
                     /locus_tag="PKM87_04020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SynChlorMet cassette radical SAM/SPASM protein
                     ScmF"
                     /protein_id="HNQ29267.1"
                     /translation="MGKKNIPPLRILYFYLTGDCNMACRHCWIAPDFLNADTSKKALP
                     FDLFKKIVKEARDIGLTSVKLTGGEPLIHPDIIPILRYIRDEELGVSVESNGVAMTPH
                     IADLIKSCKGHFISISIDGMKRNHEWMRGVSGSFERASQGVRTLVETGINPQVIMAVA
                     GRNRDEIGSLARYARDLGASSVKYNFIMPTVRGEMMEQKGETLTVQDQIALSNWIVKD
                     LQQEIRIPLLTNIPKAFVPLSAMFTSDNACGGCGIFSILGVLYDGTFALCGIGKNVPE
                     LCFGHAKEDTVRDIWENARALNNLREHLPGGLKGICARCLMKNICLGQCIAGNYYVYH
                     DLLAGHKFCEDAAEAGLFPETRLYR"
     gene            complement(10669..11928)
                     /locus_tag="PKM87_04025"
     CDS             complement(10669..11928)
                     /locus_tag="PKM87_04025"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNQ29268.1"
                     /translation="MNYRIMVTRLHILHEKQFPSVQPIIPDLIFHTSPPLLNLIQLLN
                     RNILHNEPLSAILWIEIIHFAKKMHLMPLLYSSIKNKEDIFPPPDIYIQLKSFYRENA
                     VRNLSLTYSLRILLTEFEKNNIRAIPLKGIYLANCVYDNPSARQMNDLDIMVRWKDVP
                     DARNIVEKLGFKASDTCHLDNTYRRTKHHAPDFLHPSGTRVEIHWNILSPNGGNRGKE
                     LEDMIWLHTVPGEILGKKTLFLKPELSILHSTIHLTFLHMFNSGIRDIYDIDSVYRKF
                     RGTIDWDFLVSFARRFRFERALHLVLALTDIITGSDTLSELKKNGLYLEIPNKILIFA
                     ANEIIRGGSKDSLSIKLLTSNPGPFATTRALVNWLFPGRDEMRDKYHVPPGHFGVYRC
                     YPMLNYHRVTHFNEIVRDYSFGRWMNG"
     gene            12619..13074
                     /locus_tag="PKM87_04030"
     CDS             12619..13074
                     /locus_tag="PKM87_04030"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNQ29269.1"
                     /translation="MNLNIVIILLFSLNISLFLLFRQYFQKKPYAIIINKSEKKCRID
                     LEIADSPEKWEKGLMHRKNLPDNSGMIFVFEKPHRIAFWMKDTLIPLDIIVISSDLFV
                     IEIKKNLVPMSEELITSDELTTCIIEVNGGYSERNGIGIGDKVILDLIE"
     gene            complement(13298..15829)
                     /locus_tag="PKM87_04035"
     CDS             complement(13298..15829)
                     /locus_tag="PKM87_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ29270.1"
                     /translation="MVEKWAARSADSPAIIDTSGMIISYRDLSEIISSWKQALTHQGI
                     SNGSRVAVVLPNGSDIAATFLGITSVATCVPLNPSYSRDEFFFYLQDTGCAELLVGDN
                     YPIAAEEAAQDLGIPCYKLRPIKEILFGASPIYGEMHTHNPMITPVEPDSHPEDSALI
                     LHTSGTTGKPKRVPLRQDRICRNAAAIAGSLHLTPADRCLNLMPLFHVHGLVGCLLAT
                     LHSGGAIITTPGFQSDKIAGWLHDFEPTWLSAVPAIHHSLLRVLGNYEEFVHHLRFIR
                     SCSAPLPPSLLQKMEERFKVPVIEAYGMTEASHQIASNPLPPAPHKPGSVGIPTGYTI
                     RIQDRHGQECGPDMRGEVWIFGKNLFSGYEDNPAANAEEFHKGFFRTGDEGYIDPDGY
                     LFLTGRLKELINKGGEKVAPREIEEVFLRCTGVDQAVAFGIPHRELGEDIGLIIVPSP
                     GYELALTNLKQHALQHLAPWKVPSKIQIMDEVPKGPTGKVRRRELAALLSDLDRHHEP
                     QYHNRGGETPSILEEQIQKIWEKALQHSPIGLDEDFFSIGGYSLTAMAITTELERAFA
                     VELAPTILFSAPTIRELTAVIAGERLGGSLPTLVPFQEKGDLTPLFLVPPGHGNAFFY
                     QDFAALLESDQPFYSFSRYQMPKSTSSIEGIAARNVDEIIRIKPEGPFLVGGYCFGAV
                     VALEMAHQLQDRGLEVRGLMIIDPDQLPNGPGWRWDPQRQNWKEFPLWLMRGGPRRWL
                     WFASRFITRIICPTHLSEEQRNHLEVILSNVQMLRNYHARQYTGHALVFHHGTVDGSL
                     VTQWGMVLGKYTESVVIVGAKHHEVLDKGGEQVAEHVRFLSNSTN"
     gene            complement(15846..16145)
                     /locus_tag="PKM87_04040"
     CDS             complement(15846..16145)
                     /locus_tag="PKM87_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29271.1"
                     /translation="MHSGKGIVCDLFIIETRRLVISLFQARNLPMGKRKVSYFPIPDK
                     IMQIDGKFRLLSKHPENKSRSLGWIIIKEDTLSRSQIIRKAISIAMKVPAAHASH"
     gene            complement(16169..17866)
                     /locus_tag="PKM87_04045"
     CDS             complement(16169..17866)
                     /locus_tag="PKM87_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ29272.1"
                     /translation="MIAVILVFTALRSFCMAFIPYSSKILIDSVFGNNPVQVPASPGG
                     LVSQPVHQVITFMGSSLFSFIVVVIVVGLVIGVLDIIRTYLTIRVREGFIVSLRTSLY
                     THVLFVPIRFFRSKKTGSIISRISSDTAYLQGLVITQFPQITSSMFTLLFTIGAMIFI
                     SPYLTLVFLGVFPIMAVINYIISGKIRQLTYMELERLSEIFARMGEAIAGIETVKTHG
                     AEQREAEEIATQVRDLGDTRIGNSLISALADKSRALVAGAAFLIILWMGGNQVLSGIM
                     SLGDFVAFIAFLPIVSAALQSLLSFPLQMQGSIAAAGRIKDLFSILKEYDPSDHSEKP
                     KIAIRGEVIFENVFFSYDQRRTILRNISLHVREGEVVGIIGETGVGKTTLIQLILRFY
                     SPTKGRILLDGIDISKIRHDHIRTLISFISQDLFLFHDTIRNNIRYSIPHATDEEIIE
                     AARAAQLHETIQTFPEGYDTIVGERGLQLSAGQRQRISLARAFLRNAPIMIMDEPSSS
                     LDPETEFRLLSAFAEKSRGKTVIIITHRESILPVCDRVLQLVDGNLVERQNHHRSII"
     gene            complement(18528..18905)
                     /locus_tag="PKM87_04050"
     CDS             complement(18528..18905)
                     /locus_tag="PKM87_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29273.1"
                     /translation="MTPPGGKRHQRSIRAGKPAEWPVKHYRQHHESKEQEGWDTPSSK
                     EEYIQYSSHQDEIEKKEVDYHHEREDYEHCNEHSTDNKEKTLLPELPFFYRNEVREFL
                     DRTDRAGIPTKTSPQRQRRDDEE"
     gene            19234..19611
                     /locus_tag="PKM87_04055"
     CDS             19234..19611
                     /locus_tag="PKM87_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29274.1"
                     /translation="MADRPLGVTIIGILWIIGGLLMLLAGLGLAALGAVIAGPIGGVI
                     GLALGFVLIIIGIIEILLGIGCFKAWGWVWTVGVIITIISLVMGIITLFSDWMSGLIS
                     IIISAIILWYLFQANVKAYFGKS"
     gene            19714..20337
                     /locus_tag="PKM87_04060"
     CDS             19714..20337
                     /locus_tag="PKM87_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNQ29275.1"
                     /translation="MSAIEFLIVAITSIIAIMNPVSTAAVFSILTERFTPEQQREVML
                     NSLRISLVVLIFFALTGQVIFTVLGLTIPAFKIAGGILLVSFATGMLTAKKEVYSPEE
                     LENIAVVPLAFPLTCGAGTITVVILISSQAIGILENLLVIVAVLVAIGASYIAMKYSS
                     RILRFIGDQELRIITKFLAVFVLAIAIQFILNGISDAMPDILAHVMD"
     gene            20448..21122
                     /locus_tag="PKM87_04065"
     CDS             20448..21122
                     /locus_tag="PKM87_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29276.1"
                     /translation="MERSCMLVLLALATVLAFAAGCTSSAPGSNVMPQQTTQVPGPAA
                     TATETAQATAVATDVGTPPSASPTAPLRALVLEAADLPDGYSLIYEGEMLPGAEDCPD
                     GEFCYLEGYSVSMVTGDAENSTLVDQTISRYSGHATEETLDAVLADQFPEIMAADIIA
                     LKAPPLGDASAAYRFRFPSTGDAVEGYLVIFGKGEVHEMIMVIGTGAGENLALDLAKK
                     AASKIT"
     gene            complement(21207..21818)
                     /locus_tag="PKM87_04070"
     CDS             complement(21207..21818)
                     /locus_tag="PKM87_04070"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNQ29277.1"
                     /translation="MPNDNGGSPVLAMATLAGEIASCRKCPLCASRTKTVPGEGPVPC
                     RVMLVGEGPGRKEDETGKPFVGRAGAILSGLLEEIGLSREEVFITSVVKCRPPKNRSP
                     KRDEIAACMPYLGLQIAHLSPEVLVPMGQVAAKAILEHFGLPFPSYREVRGREYGVPC
                     NAGDRELVIIPVYHPAVITHNPPARSALEEDFQRLKTVLRASR"
     gene            22046..22693
                     /locus_tag="PKM87_04075"
     CDS             22046..22693
                     /locus_tag="PKM87_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNQ29278.1"
                     /translation="MQERYHPMLQTFLLGLIIGLTGALVPGPTLVATINSSARGGWST
                     GPRVMAGHAVVEAAIFLLIIIGLGSVNALQEYTAFIAAVGGIALLVFGFLTIRGAGNA
                     MPEGKTEQAVTNPYLAGALTSVSNPYFWMWWFSVGSALVLAAAQKAFPFALAFLIGHW
                     SADFGWYTIVSTSVHQGRRVFDERVYAWILRGCGAFLLVFGAYYLATAFAWISAC"
     gene            complement(22732..23556)
                     /locus_tag="PKM87_04080"
     CDS             complement(22732..23556)
                     /locus_tag="PKM87_04080"
                     /inference="COORDINATES: protein motif:HMM:NF019949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1724 domain-containing protein"
                     /protein_id="HNQ29279.1"
                     /translation="MDILEIYHQHSRLIHSIYNSRLKIQVLLALLEGKASLSTLRSIT
                     GSTSPALIPKIRNLEALALVEAEGYEYRLSTLGKVVAEEVKSYVTLMGGIASHQQFWV
                     THDLSGLPPRFLARIGELQVSHIQTDTTVDMFSVYTHYLAILKEAAYIHGISSVASPG
                     LAQFLSEKVDEGVPVELVVSHEVIDILKQEPYASHMKALASSDNFHVWVTKEILKVGL
                     TVTDRNLSLGLFKKESPLYDSSSDLFSSDPAAVGWGEDLFQHYRKRSTELDISAFF"
     gene            complement(23633..24580)
                     /locus_tag="PKM87_04085"
     CDS             complement(23633..24580)
                     /locus_tag="PKM87_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNQ29280.1"
                     /translation="MEYASTYHHEITGQDERRSWLDYIEKTWEFFLYSSLYLGIAAAG
                     MAYVTCLIEGAGFSLPAAAILGLVVFSVYNLNRKTDEAEDALNHASRHRITSTFSSPL
                     LAMAVIAYAAALCLAAPFGIAALGITLVPLVAGLLYSLPLLPPTTGYRRLKEIPVCKN
                     LVVAFSWGLSFSLLPVAIACIDPGPATLAAFFFIFSWTFIASVLPDTRDRAGDAATGV
                     VTIPVLFGVPGTRRLLSAFNLISGFLLLVPWTSVPSRIGAVLIAFSLMYSQACIIAIG
                     RTCHEGFLCDVVSDGQFIPIAILGFLVARGFPVFGTWVL"
     gene            24733..25281
                     /locus_tag="PKM87_04090"
     CDS             24733..25281
                     /locus_tag="PKM87_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29281.1"
                     /translation="MQKEPDTIYLGVERAPDFSLPFNQRMGIRDRGEPPVNGRCESRM
                     GEILLQFLLAHQVISHTDCGCQSDFFRQFLAPFHIAVRDVDIHILERTTLGGDEVAIG
                     SFYLCFEREGSSAGKNVAAQGLYTVRCNGCDTWVKFTSSTYQFRVHIRGPDPASVTIS
                     HNGQVMETGPLSSGTAPDPTRS"
     gene            25668..>26043
                     /locus_tag="PKM87_04095"
     CDS             25668..>26043
                     /locus_tag="PKM87_04095"
                     /inference="COORDINATES: protein motif:HMM:NF014527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29282.1"
                     /translation="MALKDDLRGTLPDEVAKRIPDGFQIIGDVAVISFPPELHEFRHL
                     VAGAIISRHRNVRTVAAREKIPRGDLRVANLEVILGVDSTTTLYREYGFCYRLDVEVT
                     FFSARLASERRRVWMQVRSGEEV"
BASE COUNT         6408 a         6384 c         6734 g         6517 t
ORIGIN      
        1 acgaaggaga agacctcccc cgtgtgcatc cggctcgcct ctttcaggag ccagccatag
       61 cctttctgca ccaggtcatc cccatcggtg agcaggaggt cggcaatcgc gagcgactct
      121 tcaaggaact tcccgtgctt tgcgggaatg atcagcgaga ctgctgccgc ccttcgcatc
      181 cagcggtttt ctgaccgggt ccagcgcttc agttcgtcga tggtgtcggg atactgttcg
      241 atgaaagccc ccatcgtgtg gttgcagaat ccgtcgcagg atgcccagtt ggtgatgtac
      301 gtcccgatcc agtgcctgaa tacggaaaaa tcctcccttt cgtagttgtc agagaggcgc
      361 tgcgcccatt cagatacaat gaacgactct tccatgtagc ccgatcggta aagatcctcg
      421 cagagtgcaa aaatttcccc tttatccctt ggtttgacct ccttccagta ttgcttcgca
      481 attgcgacca ccctggcagt cttcatcccg taacaccgta tctcctcctt gaagaacctc
      541 tgggagttct tctggatttg ggggtccgca tgttcggaca gttcttttcg gatacgagcg
      601 ataagtgggt ccatgatgaa acgtgggcga gaccctggca taaagggtga accccgcggg
      661 aggtgagagt aacccatcgg cagatcgatg aagcagaaga gtagggggct gtcccggcat
      721 acctgttctt atatggggaa aaggcaaccc ctcaggggcc ccttatgggg gcaaaccccg
      781 aacccaatcc ttatcatccc gcactgccat cccgggaaat gggagatgca gccggatgtc
      841 gacactgcgg ttcctgctcg aacaccccat cagggccagg aaggtgaaag aggcggtcgg
      901 ttcaaagtgc gaactctgcg ggaagatctc gaacacagac gatctcgaag tgcatacctt
      961 catcgaccct gggaaggaac aggagatgcc tgccgaggaa cttgaatgct tcctgctggt
     1021 cctctgccag caatgccacg aagatctcca taatctcgtt gcggggagca gggcgcagga
     1081 gatacttgtc cgtcagaggg aggaatcggt ccgaaagaag atacgtgcaa tcctcggtta
     1141 ctcccccagg ccctacaatc cgccagattc ggatgtggag ggggcataca aggacgcatg
     1201 cgcctcgaag ttcgggaacc tgatctaaaa agggatgcac atctctcttt cggcgggcga
     1261 tgaggtcaga ggttcgtgca ggtccccgcg catcatttca attttatcaa cgtgcctggc
     1321 cacatgtgat gttagcgagt ttgggggact gcactgagaa aagtaagtgg ggaaacagtc
     1381 tcatctaaat aattggagcg tgaacgcaat ggtaaccacg catgaagcag cagatcctga
     1441 tctccggggc ccttatcctg ttgtcggcgc tcctgggcgt tgcacactcc ctgctcgatg
     1501 acctcgtggt cacaaatctc ttcctcacct cgctcgtcct tgccgcttcc tacctggtct
     1561 tttccgtact actcggccag ctggtggtca ggcgcatcca ggaccagaag accaggtata
     1621 ccgcacgaaa ggtcctctcc atcatctcga tcgttgtgat ccttgtcgcg accctccgga
     1681 tatggatcac cgacacctcg tcgctcatcg tgtcgtacgg gatcataggg gcggcgctcg
     1741 cgtttgcggt ccaggacgtc ttcaaaaatt ttgtcggggg tatcctcatc atcgcatcag
     1801 ggtatttcag ggtaggcgac cgcatctcga tcggtgatca tgtcggagat gtcatggaca
     1861 ttgggatcat gaatacgacc atgatggaga tccggggatg ggtgaaggga gatcagccat
     1921 cgggaaggct cgtcgtcgtc ccgaatgcgc aggtaataaa ccacgtcttt ttcaactata
     1981 cccgggacca ctcttttgtc tgggatgaga tctcgatccc tctcacctac gaaagcgact
     2041 ggcggaaggc ccgtgatatc atcctgtcaa tactgaaacg tgagaccgat accatgaccg
     2101 agcaggcaga gatcgagatc gagcggatcg gggagaagta tttcctccca aggaaagtgg
     2161 tcgaaccttc catctacctc tcgctcacgg ataactggat cttgctcgaa gcgcggtatg
     2221 ttgccgacac acggacccgg cggatcctcc gctcgcggtt gagcgagctg attttggccg
     2281 cccttgaaac agaggagacg atcaccgtcg cttccgagac catgaccgtg accaccgtcc
     2341 agaagtgatt tccgcgcacc acgcgagtaa ttttgagatg cttcttttga ataataatca
     2401 gtctgcaatt ttcacggggc atccagaaac tccggagtga aatgcacccc caagtgtata
     2461 cggttcgtca atcctgccca cgttagatag cgaagagccc aaaaaatttt tcccgcattt
     2521 tgcactcact ccaaaattgt attggagaga acagtgcctt tttcgcagat gtgtccatga
     2581 ggccgcatcg catatcaggg tttgatgcac catctgtatg ggaataaagg atgtgaaaaa
     2641 tgtccgactt cacactcttg cctcttgccg gatccgatgg agccgaagtg atagatctct
     2701 tcaactatta cgtcaggagc agttttgcag cctttccgga gaagccggtg ccgcctgaat
     2761 tctttgacag gctaacacag gcagcagagg ggtaccccgc ggttgccgcc cgcgacaggg
     2821 atggggctct ggccgggttc gggatgctcc gtcctttcaa ccccatgcct gcgtttgccc
     2881 atactgcaga ggtcacctac tttgtcagac ctgacgtgac cggaagggga atcgggaccg
     2941 cgatgctccg tcatctcacg gcggaaggga agaaacgggg aatctccgtt atcctcgccc
     3001 acatctcctc ccgaaacgag ggcagcatcc ggttccacga aaagcaggga tttttcgagg
     3061 tgggacggtt ccgcggagcg ggaaaaaagc gggggttgcc ctttgattcc gtgtggatgg
     3121 agaagttgtt gtaaagaaaa actcacagtg taaaaataaa tacagttcat gagaataaga
     3181 ggagaaagga atcaattcat cccatgaatt aacgtgagaa tgtggagatt taaaaaatgc
     3241 cacgccgaaa aacctgatat ttaatcctga tattttatct cctttgtatc aagagagata
     3301 tcatgatctg aatggggaac cgaacctata cctgggttgt tatcttggtt tttatatgtg
     3361 gtcttctggc aggcctcggc ctttctccac tgttattctc tcaaaagcca catactgaaa
     3421 agaccgttat cgtcagcagt aatgcacctg aaccagtcgg cccttaccac caggcagtca
     3481 ggtacggcga tctggtattc ctctcgggcc agatcggtct ttaccccggg aacggttcac
     3541 ttgcagggac cactgaagaa cagaccgtgc aagcgatgga gaacctgaac gcaatattac
     3601 aggaatccca acttgatttt tctgacgtgg ttcaggcaag gatctacttc atcaatgcat
     3661 cagactggga taccatcaac aaggtctatg ggcggtattt tgagggggca tatcctgcca
     3721 gagctgccgt gaaagttgca ggattgccga aggatgcaaa ggttgagatt gagatgattg
     3781 cgcgggtttg ctgatatgat cttcgggatt gttcgtattt ccagacgcga gccggttgtc
     3841 atcatcgatc cccttacgaa tccctatatc ttagattata aaatcaggga tcatttgaaa
     3901 gggatgaaag agatatcttc ctttaatgct ccctgaagag tggccatccg gttcccgggc
     3961 ccttgtgctc gcaggattct tctttctcgt aatcatcggc ctcaagttct gtgcgtacgt
     4021 tgttacgatc atcgtcatct ccctcatcct caccctcatc gcctgccctg caatggaccg
     4081 gatgagaaaa aaggggattc cagatatcgc ctcggtgacc atcctgacac ttgttgccat
     4141 cgcagcagtc ctcgtgctca ttggtatctc tctctattcg tttggggtcc tgatgcacga
     4201 tctcccactc taccaggcgg aactcaacca gcggatggcg cagatctcgg ggatgctcgc
     4261 cgggcatggg cttccgacgg gagccatccc cccttctcct gaccttggtt cccttgtcgg
     4321 cctcatcctc tcttctgtga tgaacctctc tgacctcctc ctctacctct tcttcatcgg
     4381 ggtcgccacg tttttcatga tgctcgaagc cccccacctt gcagacagac tcacccgcct
     4441 tcccggggtc cctcctgaaa aaatggccag gctgtcgcgc atgagcgggt atatgataga
     4501 cttcgtggtg gtcaggaccg agacgaacct gatccatggc atcctctttg gtgggtccct
     4561 ctatgccatg ggggtccatg ctgccatgtt atggggggtg ctgaccttcg tcctcgcata
     4621 catcccgttc atcggcctca tcatcgcggc aatcccggcc attttcttcg cctggctcca
     4681 gttcggcctg tggggcgccg cggcagtcat cgcgattgtc tgcatcctga acctcgtggt
     4741 agagaaccct gtattctcct attttgccgc acggaagttc gaaattcctg cgctgattgt
     4801 gatactctcc gtcattttct ggggctggct ccttgggatt gcagggatgg tctttgcagt
     4861 gccacttacc ctgatggtcc tcatcacaat acagtacagc gacgacctgg cgtgggtgaa
     4921 tactctgctc ggtgtagacc ggatatttgg ggaatcaaca gaaaatacct gacactggag
     4981 gatagatgta gattccaatg cgttctgacc aatgcgtaca cttgattgta cttattgagt
     5041 cattactgcc gggggcagga tacaattgcc ctgcatatca ttaagctggt catcaggaat
     5101 gcacgacatt ctccgacttt gtggcaggtc caagcagggc ctgcaaggcc cctggagatg
     5161 gaatgggtca gatccgggag ggattctcaa cctgaccacc ctttcccggt tgctttaagg
     5221 taggcttctt tgagtgtcca gagattaaag aatgcctgtg tctgttcaat gggaggaagc
     5281 aagctgatta ggttcaattg ctctgtatga aacagctggt gtgtatttga aatgagatcg
     5341 ggtatttcct tgatttgttc aatatcgact ccaacagggt tttcgtgact gatatccagc
     5401 atccagtttt ttcctgaaaa agacgttgag aaggtatatg gacaggaagg agtgtcaaca
     5461 atgggttttc catattgtcc atatgaaagc cgtacttccc caggattgcc tgataggtaa
     5521 gatgcaagga tgtaagacct gagacgatca agggccactt taggaacaaa cctggccgga
     5581 tctttgggag gataaaattt catttcatca actggtccag ctagacatcc tccaattacc
     5641 ctcaattttt ctggaatctc aagtccccag aggtgaaatc tgacttagat gaagcggata
     5701 tgtcctgatt tgatctccca gtgactcgca tgatccatta aattcaaact tcagatttcc
     5761 tgacaaaagg atacccctat ctttttcaaa cagattcaca catgccccag gaatatcacc
     5821 atctccctaa aggagatgcc ataaggaaaa ttcgtcaaac ctgattatat gaacgatcgg
     5881 aaaacagggt tcaaaggaca gtggcatacc ctttttcagt aatttcggtt ataaaagccg
     5941 taacatcctc aagcacggaa tctggcgcat cggggtagga atcctggacc atcatactga
     6001 tatcatgaac tgtgcgtttt ccatcgatca tcttccagac tcggacaccg acagggttga
     6061 gaccgcagac ctttcctgta tccgggtcaa agaggagcgc ccagtcatca aattcctccc
     6121 tgagtacgat ttggtccttt ttccggagga cccctcttcc tgacactgtt tcgcttgatc
     6181 ccatgaattt gttattcgag attgtaggat aaaaaggttt atccgagaga aatatgactc
     6241 tttttctgtt ttcatggaaa atgatcggca gatcagatat gtgggaaaca gcatgagtcc
     6301 ctggttgctg cagaatgata tcctggattg ggaaccgata aatgaacggc tacctgttcc
     6361 cggcgatgtg ataattttca gggatcctgc aaataccggt tgtctcatca ttcatcgtgt
     6421 cgtcagcaaa taccctgatg gatcatattg cacacggggt gacaacacgc tatccaatga
     6481 tgggcatcct gtgatgctct cttcaatcat gggagtggtg accagaggta tccgggaaga
     6541 acgacctctg gatgtctcta tgggattcgc tggcctgatg catcaccaat atgcacaaca
     6601 acgggtaaaa ctactctcta tatttgagca ttttttcacc aggccttacc ggaaaatctc
     6661 acaaaaaaaa gtcatgtgtc gtatcgtccc gcagcggtat cggcagcggc ttgtcatcgt
     6721 aaagacccat gaaggataca atctccaggt gtacttaagg aagcgacttg tggggtggaa
     6781 gggagaaaag gaccaggatt ggacaatcct ccctccctac cggctgttca ttgattgggg
     6841 gaatctcccg gattgtcccg taaaattttt aaatggtgta tatacaaaaa cgcaatactc
     6901 agtcaccgat gataaccagc caacccctca agaaggggtg cgaggagatt aatcaatatt
     6961 gaagaggatc ccgataacgg aatgattgct ctatcgatta tgaaaaagga ttcgagttat
     7021 ttcgttgtca tgttgtttat ctcataataa ggatgagtat tgaaacaatg agaaaggata
     7081 gaataatcat tttcagggaa tcgggaaaag atgggatatc accatgaccg gaccatccta
     7141 cgaactcgtc attcggaacg gtcagcgatg ggctttggaa gcttcccccg atcttgagga
     7201 ctggttgcag cactttgcac gcattatgat gcttcgcgaa gtagatggaa ggagaaaaag
     7261 tccttcctgg aggttttgtt tccaggattc ggatgtaccc ttatttcatg gcggaggctg
     7321 ggtaacatca cgatcgcgct ggatgagcgg ctatgcaaaa acagatggga cagatctcgt
     7381 cactgagatt attcccttcg accgtgggga tccaaaagag gatatcgtga ggatgatgct
     7441 tgcgatccag accctttacc tcgcaatctg gccatttgga ggcattcctg tccatgcagc
     7501 acttctcgag cgtgatgggg aggcgatatt gatcgctgct cccgggggaa cggggaaatc
     7561 cacatgtgca agccgtgttc cccccccatg gcagactctg tgtgatgata ccgttctttt
     7621 gataaaggca ggtggcgtct attatgcaca cccgcttcct acttggagtg attttctcat
     7681 acgcgattta tatgacagga gatgggatgt aatgtacgcc gttccagtga cagggatctg
     7741 gttccttgaa catggggaac aagaccaggc agtcccactg ggaaaaggcc aggcggcatc
     7801 cctcctctat cagtctgcag tccaggtgag cagcatttgt ctcagaaaag aaaacaatga
     7861 cgatatatat gctgaatggc atatgggact cttccatcgc agctgtgatg tagtatcaca
     7921 tttatcctgt ggtatccttc gggctaccct tgatggctca ttctgggagt gcctgtcttg
     7981 atcgacattt ttgagcgggg aatggtgacg attggaacat acaatcccag gagggcccta
     8041 ttatcaggcc agagtgaacg ccatgcccgg aaggaggatg gaatcattcc ttgcaaatat
     8101 ccaatccttt atgatatgat agccaacatt ttttggtaat caactcaatg gatgacccat
     8161 tattagggag ataaattttg tttccattct atgaagagaa tacaatgata tggatggaaa
     8221 aggggggaag gcaataggac cgatgaaaaa tggttgaacc gagaaagctg atgaagtctc
     8281 cgaaaagcat ggacctggac atcacctccc gctgcaatct gaaatgcaag tattgcttcc
     8341 agaggaccag ccctactgat accaagaccg aactgcctac ggaggaatgg atggcttttt
     8401 gcggagaact tggtgactgc gcggtgatgt ctgtctgtat cgggggagga gaaccgttca
     8461 tcagggatga tcttcttgag atcattgatg cggttattga gaaccggatg cggttctcca
     8521 tcctctcaaa cgggggcctc atccgccccg aacacgctga atatattgcg tctaccggcc
     8581 gctgtgacta cgtgcagatc tcggttgatg ggtcctgccc cgagacacat gattccctga
     8641 gaggctcagg gtcctttgaa gcagcgatga ggggagttcg aactctccag gatgcgggcg
     8701 ttcccatcac gtcaaggatt accattcatc ggtacaacct ggatgatctc gaaaatactg
     8761 ttcacctcct tctcgatgag attggtatgc catcaatttc caccaacagc acctgttacc
     8821 ttggagttgc ccggaaaaat cccgatgaag tcgcgttgac tcccgccgag ttttgcgatg
     8881 cgatggagcg ctccctaatt attgcagaac aatacccccg ccgaataagt gctgcagatg
     8941 gtcccctggc tctgggcaga accttccagg agatggagaa ggcacgagcc gaagggcggg
     9001 caatccctgg caggggctac ctgacagggt gtgggtgcat gtggagcaag cttgccgtcc
     9061 gcccggatgg agtgattgta ccctgcaaca tgctcgccca catcgaactc ggtcggatga
     9121 atcgtgacct tttacttagg atttggcagg atcaccctga attgaaccgc ttaaggaacc
     9181 gctatacaat tccgctctcc acattttccg aatgtgccga ttgtccgtgg cagttaactt
     9241 gcagcgggaa ctgcccggcg agcagttata ctcgtactgg taatgtattt gccccaagcc
     9301 cagacggctg cctgaaacgg ttcctggaac acggagggcg tatcattcgc tttccacaaa
     9361 gcacctggaa aagagcatga cgaatcactg tgcgccggtt cggacgaaag atacagtaga
     9421 atgttgcaaa ggcaggatgg atcctgtcct gtaattaccc tgatatgtat ggacacccct
     9481 gaccctctta cgaatcccat gggaaaaaag aatatccccc ccctccggat cctctatttc
     9541 tacctgaccg gggactgcaa catggcatgc cggcactgct ggattgctcc cgatttcctg
     9601 aatgctgata cttcaaaaaa ggcgcttccc ttcgatctct tcaaaaaaat tgtgaaggaa
     9661 gcgagagata ttgggcttac ttcagtgaaa ctgaccgggg gagagccttt aatccacccg
     9721 gatataattc ccatacttcg gtatatcagg gatgaagagc ttggtgtctc tgttgaatca
     9781 aacggcgttg ccatgacacc ccacattgcg gaccttatca agtcctgcaa gggccatttt
     9841 atttcgatca gtatcgacgg catgaagcgg aatcatgaat ggatgagggg ggtctccggc
     9901 tcgttcgaaa gagcatccca gggagtccga acgcttgtcg agaccgggat caatccacag
     9961 gttatcatgg cggttgcggg ccggaacaga gatgagatcg gcagccttgc ccggtatgcc
    10021 cgcgatctcg gggcctcatc ggtgaaatat aattttatca tgcccacggt gaggggggag
    10081 atgatggagc agaagggaga gacacttaca gtgcaggatc aaatcgcact ttcgaactgg
    10141 atcgtcaagg accttcagca ggaaattagg attccactgt tgaccaacat cccaaaggcc
    10201 tttgttccgc tctcggcgat gttcacttcc gacaatgcct gtggcggatg cggcattttt
    10261 tcgatccttg gagttctcta tgacggaacg tttgcccttt gtgggatagg aaagaacgtc
    10321 cccgagctct gtttcggtca tgcaaaggaa gatactgtca gggatatctg ggaaaatgca
    10381 cgtgcactca acaatttgcg ggaacacctt cccggaggac tgaagggtat ctgtgcgaga
    10441 tgcctcatga agaacatatg ccttggccag tgcattgcag ggaattacta tgtataccac
    10501 gatctcctcg ccgggcataa gttctgtgag gatgccgcgg aggccgggct atttcccgag
    10561 acaaggttat acaggtgatg atgagggaga ggacaaatcc tgtaaatggt atatcgtctt
    10621 aagaaaatat attgtgtttt tttggcactc tgcccaaaga gcagaatgtc atccgttcat
    10681 ccatctccca aaagaataat cgcgtactat ctcgttaaaa tgggtaactc gatggtaatt
    10741 gagcattgga taacaacgat aaaccccaaa atgacctgga gggacatgat atttatctct
    10801 catctcatca cgaccgggga ataaccagtt caccaatgca cgtgttgtgg caaaaggacc
    10861 aggatttgac gtcaacaact tgattgaaag gctgtctttc gatcctcccc gtatgatttc
    10921 attcgctgca aagatgagaa ttttattagg aatttcaaga taaaggccat tttttttcaa
    10981 ttcgcttaaa gtgtccgagc ctgtgatgat atcggtgagt gcaagaacaa ggtggagcgc
    11041 ccgttcaaac ctgaacctcc ttgcaaaaga gacgagaaag tcccagtcaa tagtaccccg
    11101 gaatttcctg taaacactgt cgatatcata aatatcgcga atcccggagt taaacatgtg
    11161 aagaaatgtc agatgaatcg tggaatgaag tatggatagt tccggtttta gaaatagtgt
    11221 cttttttccc aaaatttcgc ctggtacagt gtgaagccag atcatgtctt ccaattcttt
    11281 ccctctattg ccgccatttg gagagagaat attccagtga atttctactc tggttcctga
    11341 tggatgaaga aagtcaggcg catgatgctt tgtccgacga taggtattgt caagatggca
    11401 cgtgtcggag gctttgaatc caagtttctc aacgatattc cttgcatctg gaacatcctt
    11461 ccaacgtacc atgatgtcca ggtcgttcat ctgtcttgca gagggattat catacacgca
    11521 attggcaaga tatatccctt tcaatggtat agcccggata ttattttttt caaattcagt
    11581 taacaaaata cgcaggctat aagtgagtga aagattacga accgcatttt cgcgatagaa
    11641 tgatttcaac tgtatgtaaa tatcaggagg tggaaaaata tcttctttat ttttaattga
    11701 agaatagaga agaggcatta aatgcatctt tttggcgaaa tgaatgattt caatccagag
    11761 aatagcagat aatggttcat tatgcaatat attccgattc aatagttgaa taaggtttaa
    11821 aagtgggggg gatgtatgaa aaatcaaatc cggaattatt ggttgaacag aaggaaattg
    11881 tttttcatgc aaaatgtgta aacgtgtaac cataattcta tagttcatct ttcaggatta
    11941 tccctatcaa ttattatcat atcaattgac atagtatttt ccgcacaacc tccctgtgtt
    12001 cctgatatgc gtaaatgtaa tatttttctt cctgaatcct tgtgaatcca gaaattatcg
    12061 ttattacccg cgaatcattc aaataccttg gcaaagaggt actattcttt ggagattatt
    12121 caaaacagaa tctacttaag agaaacgaaa ttttttttgg agatgataaa taaatgaatt
    12181 taggatttgt tagattaata cctatgattt gttgatactc tttatggcct gaaaccacct
    12241 tgaccaccgg cacaaatatc aacacctggg cctgaaccag tcaaacaggg cccccttgct
    12301 ccactcccac ttttacattc catcatttga ccatgtccag tttcagggtg ctcactcaaa
    12361 tcaatcagct tcggcttctc gtataccata taaatgtaaa gagattttta aatggttata
    12421 tagattgtgg tccagataga gagtgtgagt tgcacatctt gccttaaggg aatcaactaa
    12481 aaaaaagagg aaaatatcta tatgctgctc tcctgcaatt aattttggaa aaataaaaat
    12541 tttaagcact agatgatata aatagaaaca tggaataatt attatctttg cttctgacaa
    12601 aaactacact agtcatctat gaatttgaat attgtcatta tactactttt ctctctcaat
    12661 atcagtcttt tcctgctgtt cagacaatat tttcaaaaaa aaccatatgc aatcattata
    12721 aacaaaagcg aaaaaaaatg caggattgat ctcgaaattg cagattctcc tgaaaaatgg
    12781 gaaaaaggtc ttatgcatcg aaagaatctc ccagataatt ctggaatgat cttcgtattt
    12841 gagaaacccc accggattgc gttctggatg aaggatacac taattccatt ggatataatt
    12901 gtcatatcat ccgatttatt cgtgatagaa atcaagaaga atctcgttcc catgagtgaa
    12961 gagttgataa cttccgatga actaacgact tgtataatcg aggtaaacgg tgggtacagc
    13021 gagaggaatg gaataggaat aggggataag gtgattctcg atttaataga ataaagactc
    13081 tgtttaaatc ctattcctcc accaaatttc attgagattt caggaattaa tcaatcgaat
    13141 agaccagata tttttagttt cagttcccgg aattaattca catatttgta tcctgcacaa
    13201 tcaccaattt tctatatgta cacggaccga aataaaattc ctggtttttc atgaaactta
    13261 gagtgttgga taattcttcg caatatttct ggatcgttta attggtagaa ttcgaaagaa
    13321 atcgcacatg ttctgcaacc tgttcacctc ctttgtccag cacctcgtga tgtttcgccc
    13381 ccactatgac aaccgattct gtatattttc caaggaccat accccattgt gttaccaggg
    13441 atccatcgac agtcccatga tggaacacta atgcatgtcc tgtgtattgt ctggcatggt
    13501 aattgcggag catctggaca ttggagagga tgacttccaa atggttcctc tgttcttcag
    13561 aaagatgtgt tgggcagatt atccgtgtga taaatctgga tgcaaaccat aaccagcgtc
    13621 gtgggccccc ccgcataagc cacaggggga actctttcca gttctgcctt tgggggtccc
    13681 atctccagcc cgggccgttt ggaagttggt cgggatcgat aatcatgagg ccccgcactt
    13741 ccaatccccg gtcctgcaac tggtgagcca tctcgagggc gacaactgca ccaaaacaat
    13801 aaccccccac caggaacggt ccttcaggtt tgattcgaat gatctcatcg acatttctgg
    13861 cagctatccc ctcgatagag ctggtggatt tgggcatctg ataccgggaa aatgaataaa
    13921 aaggctgatc cgattcaagg agagcggcaa aatcctggta gaaaaatgca tttccatggc
    13981 ctggcgggac aagaaagagg ggggtaaggt cccctttttc ctgaaagggg accaaagttg
    14041 ggagtgaccc ccctaacctt tctcctgcaa tcactgcagt gagttctctg atagtggggg
    14101 cactgaagag gatggtggga gcaagttcaa ctgcaaaagc tcgttcgagt tctgttgtaa
    14161 ttgccatggc tgtgagtgag tatcctccga ttgaaaagaa atcttcatca aggccaattg
    14221 ggctatgctg gagggccttt tcccagattt tctggatctg ttcctctagt attgagggag
    14281 tctccccccc gcgattgtga tattgtggct cgtggtgcct gtccaaatcc gatagtagcg
    14341 ctgcaagctc acgtctcctg acctttcctg tcgggccttt gggaacctca tccatgattt
    14401 gaatctttga ggggactttc cagggagcaa ggtgctggag ggcatgctgc ttaagattcg
    14461 ttaatgccag ttcgtaaccg ggggatggaa caatgataag cccgatgtcc tccccgagtt
    14521 ctctgtgcgg aatgccaaat gcgaccgcct ggtcaacgcc ggtgcagcgc aggaacactt
    14581 cttctatctc acgtggagca actttttccc cacccttgtt gatcaattct tttaaccgtc
    14641 cggtgagaaa cagataccca tcaggatcga tatatccctc atctccggtt ctaaagaatc
    14701 ccttgtggaa ttcttcggcg tttgcagccg gattgtcctc gtaacctgag aagagattct
    14761 ttccaaagat ccagacttct ccccgcatgt cgggaccgca ctcctgacca tgccggtcct
    14821 ggattctgat ggtgtagccg gttggtatac cgaccgaacc cggtttgtga ggggcaggag
    14881 gaagtgggtt tgacgcaatc tgatgggatg cctccgtcat cccgtatgct tcgattacgg
    14941 ggactttaaa tcgctcttcc attttttgaa gcagagatgg ggggagcgga gccgaacatg
    15001 atctgataaa ccgaagatgg tggacgaatt cttcataatt cccgagtacc cgcaggagtg
    15061 aatggtggat tgcggggacc gccgaaagcc acgtgggttc gaaatcgtgc aaccagcctg
    15121 caatcttatc cgactgaaaa ccaggggtgg tgataatcgc tcccccggaa tgaagggttg
    15181 cgaggaggca gcctaccagc ccatgtacgt gaaagagtgg catcaggttg agacaacggt
    15241 ctgctggagt gaggtgcaag gatcccgcaa tggcagcagc attcctgcag atgcgatcct
    15301 gcctgagtgg caccctttta ggtttcccag tggtgccgga agtatgaagg ataagggcgg
    15361 agtcttcagg gtgagaatcc ggctcgaccg gggttatcat gggattgtgt gtgtgcatct
    15421 ctccataaat cggggatgcg ccaaagagta tctctttgat tggcctgagc ttgtagcagg
    15481 ggatacctag atcctgagcg gcctcttctg ctgcgatcgg atagttgtcg ccgaccagta
    15541 attcagcgca ccctgtgtcc tgcaagtaaa agaagaactc atcacgtgaa taagaggggt
    15601 ttaatgggac acaggttgca acggaagtaa tcccgaggaa tgttgcagct atgtctgatc
    15661 cattggggag tacaacagcg acccgggaac cgttgctgat gccttggtga gttagtgcct
    15721 gtttccacga ggagatgatt tccgaaagat cccggtaaga gattatcata cccgaagtat
    15781 cgataatcgc aggggaatcc gcacttcttg ctgcccattt ttcgaccatt ccggacgtgg
    15841 tcgggtcaat gggaggcatg tgctgccggc accttcattg caattgagat ggctttcctg
    15901 attatttgtg agcgggaaag agtatcctct tttattatga tccaaccaag ggatctcgat
    15961 ttattctctg gatgtttcga taacagtctg aattttccat cgatctgcat gattttgtca
    16021 ggaataggaa agtaagatac ttttcgtttt cccataggta ggttccttgc ctgaaaaaga
    16081 gatatgacga gcctccgggt ttcaatgatg aacaggtcac agacaatgcc cttcccggaa
    16141 tgcatcgctg atgaaatggg tgctcacatc agataatgct tcggtgatgg ttctgcctct
    16201 caacaaggtt cccgtcgaca agctgaagaa cccgatcgca gaccgggagg atactttcac
    16261 ggtgcgtgat tattatcacg gtttttccgc gtgacttttc agcgaatgca gaaaggagac
    16321 ggaattcagt ttccggatcc agcgatgaac tgggttcatc cataatcata ataggtgcat
    16381 tgcggagaaa tgcgcgtgcg agagagatcc tttgccgctg accagccgaa agctggaggc
    16441 cccgttcgcc gacgatagtg tcgtacccct cagggaacgt ctggatggtc tcatggagct
    16501 gtgctgcccg tgcagcctca atgatctctt catctgttgc atgggggata ctgtaccgga
    16561 tattattcct gatggtgtca tgaaacagga agagatcctg agagataaat gagatcagcg
    16621 tcctgatatg gtcatgacgg attttcgaaa tatcaatccc gtcaaggagg atccttcctt
    16681 ttgtcggaga ataaaaccgc aggatcagtt gtatgagcgt cgtcttgcct acgccggtct
    16741 ctccaatgat ccctacaacc tctccttccc ttacgtgcaa cgaaatattc ctgaggatag
    16801 tcctgcgctg atcataggag aaaaagacgt tttcaaaaat aacctctccc ctgatagcga
    16861 tcttcggttt ctcggaatgg tcggaggggt cgtattcctt caggatcgag aagaggtctt
    16921 ttattcttcc cgcagcagcg atgcttccct gcatctgaag ggggaaagaa aggagggatt
    16981 gtaatgcagc actgacgatg ggaagaaagg cgatgaatgc cacgaaatcc ccaagagaca
    17041 tgatgccgct caatacctga ttgccaccca tccagaggat gataaggaag gctgcacctg
    17101 caaccagggc acggctcttg tctgcgagag cgctgatgag gctgttgccg atcctggtat
    17161 ctcccaggtc acggacctgg gttgcgatct cttccgcttc tctttgctct gccccgtggg
    17221 tcttcactgt ctctattccc gcaattgctt cacccatcct ggcaaaaatt tcggatagtc
    17281 tctcgagttc catgtaagtc agctggcgga tctttcccga gatgatgtag ttgataaccg
    17341 ccattatcgg aaaaacccct agaaacacga gtgtaaggta tggtgagata aagatcatcg
    17401 cgcctatggt gaagaggagg gtaaacatcg acgaggtgat ctgtggaaat tgcgtgatta
    17461 ccaagccctg gagatatgcg gtatctgatg atatccggga gatgattgac ccggtctttt
    17521 tagaccggaa gaaccggatg ggaacaaata gtacgtgggt gtaaagggag gtccggagtg
    17581 atacaatgaa accctccctt acccggatag taagatacgt gcggatgata tcaaggacgc
    17641 caataaccag tcctacaaca ataacgacta caataaatga gaaaagtgaa ctccccatga
    17701 atgtgatgac ctggtgcaca ggctggctca ccaagccgcc aggagaggcc gggacctgaa
    17761 cggggttgtt accgaacacc gaatcaataa ggatttttga tgaatacggg atgaatgcca
    17821 tgcagaatga gcgaagtgcg gtaaaaacaa gaatgaccgc aatcataccg aggtaaggtc
    17881 tgaaaagcgg gaaaaaaaac aggagatctt tccaggaata attaatggcg cccgctccct
    17941 gcgccctatc aggaatgaac cgggtaatca tggtctggac gaatcgtttt atgcggttca
    18001 acgttcatcc tttgttctcc cactttaaat tcatatgagt tcaatcgatc tgcacctcga
    18061 gatcttttct tccctgcatt tgattgttat ttaaagggat gcgcttctta tctgtcgtgt
    18121 aacaaggtta gaaagttatg aaaatggaat ggaaaataaa ttgaagtttc cagtggaaaa
    18181 tgaggcaaaa atcctgagac caatgaatgt aataaaaaca tcattggtgt ccgttcgcgt
    18241 atgcatacca aaggctattt cgattcattc tgccgaaata ctcttgaatg ccactcgaag
    18301 gggctccaag aatcctgggt tttttatatg ggattatttt aaccgtcgta gaaccatatc
    18361 ttctttataa gtcaagattc ccggtaaggg taaggattgt atttctcaca ggttccctcc
    18421 tctccggcat cctgctcttt acgccgatga caccactctt tttccccgca agcctttacc
    18481 agatgaaaga gtttgatgcc gccatcccgg ttatcatcgc agtgttctca ttcttcgtca
    18541 tcgcgacgct ggcgctgggg agaagttttt gtgggtatgc ctgccctatc ggtgcgatcc
    18601 aggaactccc ttacttcatt cctataaaaa aatggaagtt caggcaatag ggtcttctcc
    18661 ttgttgtcag tggagtgctc attgcagtgt tcatagtcct cgcgttcatg atggtaatcc
    18721 acctcttttt tctcaatctc atcctgatgc gaggagtact ggatgtattc ctctttggag
    18781 gatggagtat cccagccttc ctgttccttg ctctcgtggt gctgtctgta atgttttacc
    18841 ggccattctg ccggtttgcc tgcccttatg gagcgctgat gtctcttgcc gcctggagga
    18901 gtcacttttg cgtagtgaaa acagctgcct gtaaagactg cagaaaatgc gatgatgtgt
    18961 gtcccaccgg caaggttctt tcaggccatg aagggaatga gtgttatctc tgcgggcgct
    19021 gtattgatgt atgtcctgtg cagggcggaa tgatctattc aaggcggagg aacaagagat
    19081 aaccgttccg ggtgaaagga caatccgata ttcttcaacc acctcctcaa ttcgaacagc
    19141 aatgccactt ccggggggat ggatcgaacg ccagcgaatc gtctctcctt ccgcgcccat
    19201 acttttatta tctcctgctg taaagccaca catatggcag acagaccatt aggagtcaca
    19261 ataattggta tcctctggat tattggagga ttgctcatgt tacttgccgg gcttggcctt
    19321 gccgctctcg gtgcggtgat tgccggccct attggaggag tgataggact ggcgctgggc
    19381 tttgtcctga tcatcatcgg aatcatcgag atcctccttg ggatcggctg cttcaaggca
    19441 tggggctggg tgtggacagt gggcgtcatc atcacgatca taagcctcgt catggggatc
    19501 atcactctct tctcggactg gatgtccggg ctcatcagca tcatcatttc tgcgatcatc
    19561 ctctggtacc tcttccaggc gaacgtgaaa gcgtatttcg ggaaatcctg acaccaaggc
    19621 ccctaaaaac tctttttttt gaataacgcc ttcctggagg ggtcaaaagg tatatctgct
    19681 cttcggccca tgccctggta ggactcaaaa gatatgtctg ccatcgagtt ccttattgtc
    19741 gctatcacct cgatcatcgc gatcatgaat cctgtcagca ccgcggcggt cttctctatc
    19801 ctgactgaga ggttcacccc ggaacagcag agggaggtga tgctcaactc gcttcgcata
    19861 tcacttgtgg tgctgatatt ctttgctctc accggccagg tcatcttcac ggtcctaggg
    19921 ctcaccatcc ccgcgttcaa gatcgccggc ggcatcctcc ttgtctcgtt tgcaaccggg
    19981 atgctgacgg caaaaaagga ggtgtattct ccggaagaac tcgaaaacat cgccgtggta
    20041 ccgcttgcat tcccgctcac ctgcggtgcc gggacgatca cggtggtgat ccttatctcg
    20101 tcccaggcca ttggcattct cgagaacctg ctcgtgatag ttgcggtgct ggtcgcaatc
    20161 ggggcatcgt acatcgcgat gaagtactca tcgcggatac tccgtttcat cggcgatcag
    20221 gagttgcgga ttatcaccaa gtttcttgcc gtatttgtac tcgccatcgc gatacagttc
    20281 atcctgaacg ggatctcgga tgcgatgccc gatatcctgg cccatgtcat ggattaaaat
    20341 gtccagcgta tcgagaaata ccctcatgtg cctttttttg gtctatggac ataccaaaac
    20401 aaatttttcc tcctactcgc aaatgccgtt accacaggaa gattctcatg gaaagatcat
    20461 gtatgctggt tctcctcgcg cttgccacgg tgctcgcgtt cgcggcggga tgcacatcaa
    20521 gcgctcccgg ctcaaatgtc atgccccaac aaacgactca ggtcccggga cctgctgcca
    20581 cagcaaccga aaccgcgcag gcaaccgcgg ttgcgacaga tgtgggaact ccccccagcg
    20641 cttcccctac agccccgctc agggcactgg ttctggaagc tgccgatctc ccggacggat
    20701 attctctcat ttatgagggg gagatgcttc ccggagccga ggattgcccg gacggtgaat
    20761 tctgttacct cgagggatac tctgtctcga tggtaacagg agacgcggaa aactccaccc
    20821 ttgtcgacca gacgatctcg cggtattctg ggcatgcgac ggaggagacg ctggacgctg
    20881 tcctcgccga ccagttcccc gaaattatgg cagcagatat catcgcgcta aaagcccctc
    20941 ccctcggtga tgcgagcgca gcatacaggt tcaggttccc ctcgacaggg gacgctgttg
    21001 aaggctatct cgtcatcttc gggaagggag aagtgcacga gatgatcatg gtcatcggga
    21061 cgggcgcagg ggagaacctt gctcttgacc ttgcgaaaaa ggcggcttca aaaattacct
    21121 gaagaaggga gagtgtgggg tctcactctt atcctcaata acctcatttt tcctgggaat
    21181 ggaacaatct gcgtggatcc ctcaattcac cggctggccc ggaggacagt cttaagcctc
    21241 tggaagtcct cttcgagcgc tgaccgggcc ggcgggttgt gggtgatcac cgccgggtgg
    21301 tatacaggga tgattacgag ctcccggtcc ccggcattgc aagggacacc gtattccctc
    21361 cccctcacct ccctgtatga tggaaagggc aggccgaagt gttccagtat cgccttggcg
    21421 gcgacctgcc ccatcgggac aagtacttcg ggtgagaggt gagcgatttg cagtccgaga
    21481 tagggcatgc atgcagcgat ctcgtccctt tttggagagc ggtttttcgg cggacggcac
    21541 ttcactacgc tcgtgatgaa cacctcttcc cgggagaggc caatttcttc gaggagacca
    21601 gagaggattg cgccggcccg gccgacgaac ggtttccctg tctcatcctc cttcctgccc
    21661 ggcccttctc caaccagcat cacacggcag ggaaccggcc cttcacccgg gaccgttttc
    21721 gtccgcgagg cgcagagcgg acatttccgg caggacgcaa tctcccctgc aagcgtcgcc
    21781 atagccagga ccggagagcc gccattgtca ttgggcatag agggtcacca tggggtggca
    21841 tgtacagtac ataaggcaat ccaggggatg tgggatggga ttcatcgggt gggaaaatac
    21901 ctcttgcatg aaggaatggt ggaattgcat ggggcaagga tgcttcgatt caagccggca
    21961 tgggacaggg aataatccgg atccgtggca gcaatcccag ggcacgggga gaccaaatac
    22021 cccataacct cacggggcaa atatcatgca ggaacgatat caccccatgc ttcagacatt
    22081 cctcctgggc cttatcatcg gtcttaccgg ggcgcttgtc cctggcccaa cgctcgttgc
    22141 caccatcaac tcatcggcaa ggggcgggtg gagcacgggc ccccgcgtca tggcaggaca
    22201 tgcggtcgtc gaggcggcaa tatttctcct tatcatcatt ggtcttggct cggtgaacgc
    22261 attgcaggag tatacggcgt tcatagcggc agtagggggc attgcgctcc tggtctttgg
    22321 attcttgacc atcagggggg caggaaatgc gatgccggag gggaagacgg aacaggcagt
    22381 cacaaatcct tatctcgcag gagccctcac cagcgtttca aacccgtatt tctggatgtg
    22441 gtggttctcg gtcggcagcg cactggtcct tgccgcggcc caaaaggcgt tcccgtttgc
    22501 cctcgcattc ctgatagggc actggtcagc tgatttcggg tggtacacca tcgtatcaac
    22561 aagcgtgcac cagggacggc gggtctttga cgagcgggta tatgcctgga tattgcgcgg
    22621 ctgcggcgcg ttcctccttg tttttggcgc atattacctg gcaacagcat ttgcgtggat
    22681 ctcggcctgt taaccaggat tgccggggcc ggactcctgc cagggcagtc cttaaaaaaa
    22741 ggccgagatg tcgagttccg tgcttcgttt ccggtaatgc tggaacagat cctcgcccca
    22801 tccgactgcc gcgggatcgc tgctgaagag atcagaggag ctgtcgtaga gggggctctc
    22861 tttcttgaag agcccaagtg agagattcct atcggtcact gtgagcccaa ccttcaggat
    22921 ttccttcgtg acccagacat ggaaattatc agaagatgca agagccttca tgtggctcgc
    22981 atagggctcc tgcttcaaaa tatcgatcac ctcatggctt acgaccaact ctaccggaac
    23041 cccctcgtcg accttctccg agaggaactg ggccagacca gggcttgcaa cagacgatat
    23101 gccatggata taggcagcct ccttgagtat agccaggtaa tgcgtgtaga ccgagaacat
    23161 gtcaactgtc gtatcggtct ggatgtgcga cacctgcagc tcaccaattc tcgcgaggaa
    23221 acggggaggg agtcccgaga ggtcgtgagt gacccagaac tgctgatggg aggcgatgcc
    23281 ccccatgagg gtcacatagc tcttcacttc ttctgcaacc accttcccaa gggtactcag
    23341 tcgatattcg tacccctcgg cctccaccag tgcaagtgct tcgaggttcc gtatcttggg
    23401 aatgagggcc ggggaagtgc tgccggtgat gctccggagt gtcgacagcg acgccttgcc
    23461 ctctaggaga gccagcagca cctggatctt gaggcgcgag ttgtagatgg agtggatcag
    23521 cctggaatgc tgatggtaga tctccaggat atccatatca gatacatgta ttgctgaccg
    23581 ggaaatatgt cattcgatcg gaatccgaca gggacaatcc aacaagggat cttcatagta
    23641 cccatgtccc aaagacgggg aaccccctgg caacgagaaa ccctaatatc gcgatgggaa
    23701 tgaactgccc atctgacacg acatcgcaga gaaacccttc atggcacgtg cggccgattg
    23761 caatgatgca tgcctgtgaa tacatgaggg aaaaggcaat caaaacggct ccaatccgtg
    23821 acgggacact ggtccaggga acaagcagga gaaatcctga aattaaattg aacgcggaaa
    23881 gaagcctcct ggtccccggc acgccgaaga gaacggggat ggtgaccacg ccggttgccg
    23941 catcaccggc acggtccctg gtatcaggaa ggaccgacgc aatgaatgtc cagctgaaga
    24001 tgaagaaaaa cgcagcaagg gttgcggggc ccgggtcgat gcaagcgatg gcgacaggaa
    24061 gaagtgagaa ggagagaccc caggagaaag ctaccaccag gttcttgcaa accggaattt
    24121 ccttgagacg acgataccct gtcgttggtg gaaggagcgg caagctgtac agcaatcctg
    24181 cgacgagagg gacaagggtg attccaaggg ccgcgatccc gaacggcgcg gcaaggcaca
    24241 gggcggctgc gtacgcgata actgccattg cgagaagcgg gctggagaat gtactggtga
    24301 tcctgtgacg gctggcatgg ttaagggcgt cctctgcctc gtcggtcttc cggttcagat
    24361 tataaaccga aaagaccacg agaccaagta ttgcggcggc cggaagggag aagcctgcac
    24421 cttcgatgag gcaggtcacg tatgccatcc ctgcggcggc gatgccgaga tagagcgagc
    24481 tgtacaggaa gaattcccat gttttttcta tataatcaag ccaggagcga cgttcatcct
    24541 ggcccgtaat ctcatggtgg tatgtacttg cgtattccat gagatgaaga attgccgatg
    24601 agattcacat tcattgtgat gatgaatatt catcgctgcg atgaatatga aactccgtga
    24661 taaatatggc accacacatt caacacactc actggagtca gggttcactg gagagagtat
    24721 gcttcaacga tcgtgcagaa agaaccagac actatctacc tgggcgtgga gcgtgctcct
    24781 gacttcagcc tcccctttaa ccagagaatg ggaatccggg acagggggga accaccggtg
    24841 aatggacggt gtgagtcccg gatgggggag atcctgctcc agtttctcct tgcacaccag
    24901 gttatttcac acaccgattg cgggtgccag tcggatttct tcaggcagtt ccttgctcca
    24961 ttccatattg ccgtgcgcga tgtggatatt catatccttg aaaggaccac tctcggtggg
    25021 gacgaggttg caattggttc tttctatctc tgttttgaga gagagggttc ttctgcggga
    25081 aagaacgtag cagcacaggg actgtatacc gtccgttgca atggttgcga tacctgggta
    25141 aagtttacat cgagcacgta ccagttcaga gtgcatatca ggggccccga tcccgcatca
    25201 gtcaccatat cccataacgg acaggtcatg gagacagggc cactctcttc aggcacggca
    25261 ccggatccca cccgttccta aaaaaaactt gagtggaggg agtcgtactt catgagtggt
    25321 ctctccttct cgtggaagtt tttatacggt tcttcgctat ctgttgtggg caggattggt
    25381 gaatcggaca cattttgtgg gggctgtccg accccgcacc cccatatgcg atacacgccg
    25441 ccgcccccga acataccgcg gggtatacgc cctcgacccg gggcgtcctg cggtcacctt
    25501 cagatttacc gccattactg agtaatctgg gagaagttct gtgcggaagc acggcggcgg
    25561 ttggagtgac tgaattgata tgcagatacc aacctcgatt cagtctcccc acataaaaca
    25621 gcgaggaggc ttttttcatt catgcgacaa tcacctggta tcatacaatg gctttgaagg
    25681 atgatctgcg gggaacgctt cctgacgagg ttgccaaaag gatcccggat ggcttccaga
    25741 tcatcggcga cgttgcggtc atctcattcc ctcctgaact gcatgaattc aggcacctgg
    25801 tagcaggagc gatcatctcc aggcatcgca atgttcgaac cgtcgcagcc cgggaaaaga
    25861 tccccagggg agacctgcgt gtggcgaacc tggaggtgat tttgggtgtt gactccacca
    25921 ccactttata cagggaatat gggttctgct acaggctgga cgtggaagtt acgttcttct
    25981 ctgcgaggct ggcatctgaa cggcggcggg tgtggatgca ggtgagaagt ggcgaagagg
    26041 tgc
//
LOCUS       DAOQYR010000020        25542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_2256_length_25542_cov_8.618825, whole genome shotgun sequence.
ACCESSION   DAOQYR010000020 DAOQYR010000000
VERSION     DAOQYR010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 25542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25542
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2256_length_25542_cov_8.618825"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1816)
                     /gene="infB"
                     /locus_tag="PKM87_04100"
     CDS             complement(41..1816)
                     /gene="infB"
                     /locus_tag="PKM87_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNQ29283.1"
                     /translation="MTQKTHIRTPIVCVLGHVDHGKTSLLDRIRGSSVVSTEDGAITQ
                     HIGATIVPIDAIRKMSGSMEKLALNVPGLLFIDTPGHHAFTTLRARGGALADMAILVV
                     DINQGFQPQTIEALQILRTYKTPFVIAATKIDRLHGWRVHRDEGFLASFSKQNDRVRE
                     LVETRVYEIVGELSDLGFSGERFDRVSDFRRNLAIVPVSAHTGEGVAELLMVMIGLAQ
                     RYMEQDLSLSVEGPGMGTILEVKEEKGLGTTLDVILYDGKLAVGDELAVARQDGIVVT
                     KVRALLQPRPLKEILVEDRFERVKSVTAAAGIKVSAPNLEGAIAGSPVQVLRGNQEEV
                     IERIRREMEEVNVRLSPEGVIIKADTIGALEALCKELDEKDIGVLRAEVGSVSRHDLI
                     DAETIKNPYFRVLIAFSTSLLPDAADMVKEPGYPVKVFEGRVIYKLVDDYIQWRDELK
                     RKTEQQRFEQIIHPAKIRVLPHCIFRQSNPAVVGVRVLGGTLRTDVNLIQPDGRKVGH
                     VKSIQLNQETIREAQTGAEVAISVEGATVGRQLDVEDDLLVDIPERHVKVLEKEMLPH
                     LPVHTQEVLAEFTAMRRRENPFWGK"
     gene            2033..2105
                     /locus_tag="PKM87_04105"
     tRNA            2033..2105
                     /locus_tag="PKM87_04105"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2067..2069,aa:His,seq:gtg)
     gene            2362..3867
                     /locus_tag="PKM87_04110"
     CDS             2362..3867
                     /locus_tag="PKM87_04110"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNQ29284.1"
                     /translation="MRSTTLQYIYLLLVASLIAGMIISSGCTNTEKKPGRLDGFDEFV
                     TAKMAEYEVPGAVVGIVENDSVVYLKGFGVREIGKPDKVDPDTRFQIASVSKYITAGA
                     IGSLVDEGKLDWDEPVVTYLPNFALKDAYAGEHATLRDLLAHRTGLRHFDGDLLGRLG
                     YSNAELLERMRYLEPGTSFRERYIYSNAGFFIAGEAAAKVDNRSWEDLTDARIIQQLN
                     MTRSGAHPETLYLDDNHVSGHAGMGGDVHVIPLEQASLPAAGQVVSTGRDMTQWLRMM
                     LNDGRVDGTQVLTPETVKEIHAASLVAGHGGPLSDPNAAVGLGCDSYNFLDTRVIEKN
                     GALDGVRAIVILIPGMKNGIVVIANKQLTVFPEAIRDEFLERYIGKSGVDLQANEKTN
                     QVGWNSLIITVERPANPGPASINQSAIFGTYTSELYGTMQVKAGSDAENMTIELGPVK
                     YPGTLMHVTNDTWYLSFPNPDDQVGYLTFEVDPSGSVSGCSSEEFGMFARL"
     gene            complement(4071..4232)
                     /locus_tag="PKM87_04115"
     CDS             complement(4071..4232)
                     /locus_tag="PKM87_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29285.1"
                     /translation="MNQNRGRRGGHCGGTMPGAVCVCPLCGYRIERPQVAPCYTFHCP
                     RCEAQMVPG"
     gene            complement(4262..4669)
                     /locus_tag="PKM87_04120"
     CDS             complement(4262..4669)
                     /locus_tag="PKM87_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29286.1"
                     /translation="MSTEVKAGKLVFVVGIVIGCALVFLLVSAGAASTSSRLIPIHLV
                     AEYLYILLALKITGLVLAIFSVYFAEKKCIQRAGVIGLVASLLPPVDILFLLGSLLFF
                     VSPEERGGTLARDACPGTQGEERYTDSDEIRVV"
     gene            4925..5797
                     /locus_tag="PKM87_04125"
     CDS             4925..5797
                     /locus_tag="PKM87_04125"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNQ29287.1"
                     /translation="MQRFSLKCAILCAILILSVCSCVEAQVEGTADAGIYRFHGNVEG
                     AEVYVDDQMVGTIKNGLLDLPVSVSGTPYQTYTFKKEGYYTYNGTINTVPAKGQVFHI
                     YVSMTAMPLVEYSRVHLLVSPPDAKVTWDGEPAGDVPPTGILIIYNVAPGKHTVKVTK
                     EGFLPIEQALTVTGNDVMRVPLSLTPVPLGSITFESVPAGASVYLDGQFRGMTPLSLH
                     DVPAGSHAVMITSEGFQDVVSAVEVTGGKTTLVSETLNPGTPVPGSTRAALSPMVLAG
                     GLAIAGLLVAYRRP"
     gene            complement(5932..7188)
                     /locus_tag="PKM87_04130"
     CDS             complement(5932..7188)
                     /locus_tag="PKM87_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNQ29288.1"
                     /translation="MEIAERKESGVVVLVCRGRLDGHGAMVLEQAAKEALHDDDRSLV
                     LDMKNVPYLSSAGIRVMLVLQKRLRDRGGKVALAGTGDFPRKVLEMAGFLSIFPDYPT
                     AEEAVRVLGKAEERASLLADLQSPSFEVNGARVRIEQGSTRPSALRLWGDMDVVLNSR
                     IDAPHLAVIPFSGIEYALGLGALGKSPEDVLPFLGEMVALHGSLVWLPADGNDTPDFF
                     TPVKDTGEVRIFSGYSVSLEGPFREFVILESIPREGMRLGEIYRLLIERAKKEHLEFR
                     GVLAVAWWAVLEGLQSQDIPTRNPSVQDSRFAQGAIPRYRGDTLVGFGIAVDLDLAEK
                     HFDRAVLNSFFSPHGEENEGGPRMITHTHGVVFRNVAYDPGASLEGQMKKILGQGEFV
                     DMRHLLDETRIRKAKIGIAPVSSLIT"
     gene            7304..7804
                     /gene="msrB"
                     /locus_tag="PKM87_04135"
     CDS             7304..7804
                     /gene="msrB"
                     /locus_tag="PKM87_04135"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNQ29289.1"
                     /translation="MKDPSKDPSRICIFSLSTGKLELVDRVVLSQEEWQRRLSAGQYR
                     VARMKGTEPAFSGEYHDLHDVGLYRCICCGTDLFASYSKFDSGTGWPSFSAPVHMNNV
                     GFHEDRSFGMVRTEVTCARCDAHLGHVFDDGPPPAKTRYCINSASLSFVPAHGKNREK
                     GDMDGR"
     gene            7912..8718
                     /locus_tag="PKM87_04140"
     CDS             7912..8718
                     /locus_tag="PKM87_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNQ29290.1"
                     /translation="MAEGQGTILTADDAEFLEELSEYLEILGNSTRLRILRLLEKNPL
                     DARSVSIATGISYENTKKHLERLLHAGLITRSPGLGRETSRGVHPVWEYSPVPDVIRR
                     VQRDLGFLFALPPHAGWSGLHGQVSTVRQRLSLEWGTLEKLLLVMGGEWDGRTLPLAS
                     PCTALGRVEPGVDASLPRCGCISFPESYRSLTRISRPHALITERDGRCVLEDRGSTGG
                     TYLNGKRIAERQQVLLRDGDRISLSLGEFGATLLFLHKPDQDTGEPGAGM"
     gene            8715..10481
                     /locus_tag="PKM87_04145"
     CDS             8715..10481
                     /locus_tag="PKM87_04145"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNQ29291.1"
                     /translation="MKHPLIILTVLLAGMMVCSQFCSAWITYESNASRGIPSELVEKN
                     KAIQEKYKEIAESGLPQFIKPTNPFPLPATTVPTVVVTSAIPTDYPTPVPTTASPEPT
                     TNVTPIASGDQEEPATTAAFFSAVLVLVLATISAILNLAVRWRDREPPGLAAPWWSRW
                     HGVIAGVHALMAVIFFLEGLVFIDSGMLWFLSSGREIAWIILGGFLLVNGAVSSAGLA
                     ISSFFRRERRNVSIVHAALLAAGLAVYAVFLFAPIFPFPQAVPVAAFLGGLGIILIQA
                     NVPRETAAEPPSAYTDTMLFEDNPAAATPFFPPELSGRYFDARFLHQGGIARVFSARR
                     RSDGTMVAVKVPIHTDEQTGRSLLREMSVWKTLIHPGIVQVNAANILPVPFVEMEYLP
                     GSLEDIPLPMEPGDAAALVRKVAEALLFAHSGGVIHRDLKPGNILLTARGEPKIGDWG
                     LSRNDGIPSETTLHGFSLSYAAPEQLDPGRFGRSTGKTDIYQIGIIFYQLLTGTLPFP
                     GESIAEVTTERLSGISKPFFGNNPSLSMFGPVIGRCLETSPDERYSSLKDLLSDLEAA
                     ERKYRALAGKSPGHEEEDFGSG"
     gene            10474..11751
                     /locus_tag="PKM87_04150"
     CDS             10474..11751
                     /locus_tag="PKM87_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNQ29292.1"
                     /translation="MVEHPGDAEEPHFRHPFRQDGRARVPVALVRCAEYQPDQVFRAL
                     NEAITLAGGIDSFVTPGTRVLVKPNLLQGLPPERAVCTHPVVVGAVIRLLKEHGCTVT
                     LADSPGAGIRYTPTSLARQYERAGYEGLALDPGCVLNLDADSRLVKYPEGKVCREFFL
                     ISPFFSSDHVVVVSKAKTHVLTLLSGATKNVFGLVPGLEKPLLHSRFQTGERFGDMLV
                     DLNLLVRPSLQVADAIVAMEGDGPTSGSPRHLGFLLASPSPLALDMVLCRLMSLDPLD
                     VPYLKCAIVRGLIAPDGRDIELLGDPLPPSMEHPVRKPGTYINHSGSMPVTPLFRAVH
                     TLGSLCAMRPIVTHQKCTGCAKCATICPARAITVSGGRARISLHRCIRCYCCHEMCSE
                     GAISLERSLAGSLLYRVMQACGWAQGKKGHPLS"
     gene            12225..13013
                     /locus_tag="PKM87_04155"
     CDS             12225..13013
                     /locus_tag="PKM87_04155"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNQ29293.1"
                     /translation="MRQRTFTTVIISLTILALSCSALCGACAGGSITATIGDSIPLNG
                     TVQLADTVYLFVTGPGMPPNGARMENSNAAVITGDPDTFTQVMVENDRWAYTWNTGRV
                     SGGLAPGSHTVFVSTQPAAANALSGVNYAEIEVILHAAVTTATLEVESSPADAAIYLN
                     GKYSGNSPRVFRDLAPGEYAIRLEKQGYTDETGSVTLAAGDDVKFTRTLSPEGTPDTT
                     VATLPATFPKVGETPPPAPTTTPLTSACIAGTLVLAGLFFRKPD"
     gene            13173..13328
                     /locus_tag="PKM87_04160"
     CDS             13173..13328
                     /locus_tag="PKM87_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29294.1"
                     /translation="MCTVGGPVDMDLPPERVKGKNYRCKDCGEKFKGVGRHPMCPSCQ
                     SEDVEEV"
     gene            13548..13991
                     /locus_tag="PKM87_04165"
     CDS             13548..13991
                     /locus_tag="PKM87_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29295.1"
                     /translation="MRLQPIFLVVLVCGMIFITGCTQQEVQVTPTPTPTPEAPPPDIS
                     PLPTDTVPTEYRVFVTVTRNTYSFHPLIKVEFRGGQGMGLLSSLDAEVIREDGSAGTA
                     SLQRPAIGEFMEVQGSTGKDRVIVHAHMMNGARYRIYDDVLEFRT"
     gene            complement(14041..18327)
                     /locus_tag="PKM87_04170"
     CDS             complement(14041..18327)
                     /locus_tag="PKM87_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ29296.1"
                     /translation="MISVLLVDDEPALLDITQIFLEKDGKMSVTLSESAGSALRMLDT
                     ERFDIIVSDYEMPQMNGIDFLKAVKARGVDIPFIIFTGRGREHVAIEALNIGASFYLQ
                     KGGDPKSQFAELRNMIVQAVQRKKAEEEVRLNQHRLQAMIDLHQMSAAHKRDICTYAL
                     ETAISLTGSTMGYIAFLNEEETVLSMYAWSKTALSECRVAEKQVIYPVEKTGLWGEPV
                     RQRRPVITNDYAAPNPAKKGHPEGHVKMIRHLGVPVMDGKRIVMVAGFGNKRSEYNED
                     DIRQVTHLMGGLWQILKRKRIEAELVESEKKFRSYFDLPLVGIAITSPDMQWINANQK
                     LCHMLGYSLDELKALKWSDLTPPEELAKELELYTRVVSGESESHTVKKRYIRKDGRLI
                     DVEVSSLPVRKENGELDYFVALIHDISPLMQAKEELEASNNQMAATLEELRATQDSMN
                     DYCRRLEQEEAALRRSEEKFRTMVETAPSMLIITDLSGRITYASPNVRDFTGYLPEEF
                     EGQALSLVHPEDRERVKDDISTAFRELRGVRNLEFRSQKKGGEVWYASSSLEPLRDPQ
                     GAIQGFLIQTIDITSRKKAEEALLKSEEKYRELVESINDIVFSLDSDGVVTYASPAVV
                     RILGYLPEEVTGHRFTEYLHPSDIQEVMEGLALLQKERNSKPFSVEWLVKTKVGDERW
                     VRISLRTLWDGERYSGCTGTITDINEQRRAGEELREREALLQGIFRAAPVGIGIVQDR
                     VLSWVNAQVARMTGYSPEELAGRSARILYESDEEFVRVGEEKYPPMWKTGVGSVQSRW
                     VRKDGTVFDVLISSSPLNPVDRHSPIVFTVSDITDQKRAEAAARAMERKYRELVDDIP
                     DILYTADLEGHITFMNQAGLGAFEVTEEEMIGQTFEQNLHPEDIRAGIAIFQGILQTG
                     EPVMNFECRFVARRGEGRVFPVINNVSVMRDEHGRIVGTRGIALDITEKKKAEDELRE
                     REERLQMIAKQVPGSLWTTDTDLVFTSSYGAGLGDMGLLTNQVVGMKVEAFHGNSPGA
                     LEAVSAHRRALNGESVSYTAYYRDRVYKAHLEPLKGNDGHIIGTLGIAFDITDLQKAE
                     EALTESEHLLRTFINAIPEPACLIDAEGRIILANAAFCSRYSVPDTAVAGMNAYTLVN
                     GEMGGTMQARVGDVFSTGKEIRFEIAQKDRVSLANVYPVRDSIGKVTRAAVFSVDITD
                     QKKAQEVLSLLNKKLSLLSRITRHDALNRIAVLASYLELMEEQTDDPLLITYIRKEKR
                     GIEALQALLAFTESYQNVGMKSPSWQDVSAAVRRGAELQDLVHVTIYLDTEGLEIYAD
                     PMLEKVFFSLIENAVRYGGSLTRITFSSQEDEGGLLLVCEDDGTGIPAEQKEAVFSLG
                     VGQNTGYGLFLVREVLAITGFSIRETGEPGKGARFEIRVPHGAFRRSASP"
     gene            complement(18439..19203)
                     /locus_tag="PKM87_04175"
     CDS             complement(18439..19203)
                     /locus_tag="PKM87_04175"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HNQ29297.1"
                     /translation="MRVILKTKPRAKPRIPIEAEEIIPQNFLRKNDIKIFEGNKERSL
                     DDLFFVTREGPAVSPDQVEVILTGDTGVIKRVGEYMNAGRIIIEGDIGMHCGNFMSAG
                     EILIRGNADAWLGREMRGGRISCEGNAGHYCAAGYRGEKRGMRGGTVEVKGNAGDFCA
                     EYLSGGEVIVHGNCGDMPGVEMRGGTLVIGGDCLRPCGNMTGGTCMVFGQARQFLPTF
                     RRNGSQEAEYAGKRYRMNVFRGDVANRGKGTLMVRG"
     gene            complement(19200..20903)
                     /locus_tag="PKM87_04180"
     CDS             complement(19200..20903)
                     /locus_tag="PKM87_04180"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HNQ29298.1"
                     /translation="MTPGLLIKNAFVIDPLSGVKGEIMDIAISNGRIVDEAPRDAEVI
                     DAAGCLTLPGGIDSHSHVCGTKVNFGRYMSPEDMRAGRTARKGVMHVTSGYSVPTTFG
                     NSYRYSTMGYTTVLEGAMAPLEARHTHEEFTATPHQDMLANTLFDGNWSIMEAVREKD
                     VKRAAAIVAWTLAAVRGFAIKLTNPGGTEAWGWGEDVHSIQDLVPHFEVTPAEIIGTM
                     IRANEMLNLPHSVHLHCNNLGVPGNFRTTLDTIDLVPDLNKERQSLYLTHVQFHSYGG
                     STWRDICSRSEDIARAVNGKPQVVMDMGQIMFGRTTTMTADGPMEFKLFMLHHNKWSN
                     HDVELETGSGIIPVYYGRKSLVNCVMWSIGLELALLAKNPWQCLLSTDSPNGAPFVKY
                     PEIIALLMSQRLRESERKMIDPRTEHFVPLFAIERELDWYEIAVMTRAAQAKALGITR
                     IGKGYLAPGAEADVAIYPISPDQVDPSVDYQQVMAAFSRSRFTIKRGRVVARDGEVIV
                     EGANSTIWSKAHVPPEYDMGTDPEFTKKFEQYYTVRMRNYPVQDVYLPRGLRIDTEAS
                     L"
     gene            complement(20900..22186)
                     /locus_tag="PKM87_04185"
     CDS             complement(20900..22186)
                     /locus_tag="PKM87_04185"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HNQ29299.1"
                     /translation="MRIEDVVCSFCGCLCDDLVVEVEGTRVINVENGCTLANEKFMGD
                     HRLEAPIRRSGGKWEKVSYEEAIAETAGILLDADRPLLYGWSGTYGEAQTIGVHLAEV
                     LGAVIDNTSTVCHGPSIMAIQEVGHPGCTLGQVKNRADVVVYWGANPVDAHPRHMSRY
                     TTYCDGFFVKNAFRERKVVVVDVRETESSQVADEFVKVNPGGDYAVLSALRAIVRGKR
                     DIIPPDVEGVSREQLFRVADMCIAAKFGAFFFGVGLTMAPGKNKNIRNAIELTEELNR
                     HTKFTISPMRGHWNVYGSNEVFTWITGYPFAVDFARGIAFYNPGETTSVDILRRGECD
                     ACLVVASDPGAHFPRACAEHLARIPTVQIDPHVNVTTHLSRIQIPVAVAGIDAEGTAY
                     RMDGVPIRTRKLFQGRYPDDLEVLEKIYAIVKEAKG"
     gene            complement(22176..22565)
                     /locus_tag="PKM87_04190"
     CDS             complement(22176..22565)
                     /locus_tag="PKM87_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNQ29300.1"
                     /translation="MKFLFNTGRTIRQGSYVERKNAPSYREEASAIRMNPVDMFEMMV
                     EEGDRVRVKSSTGEVVMKARADPGLVRGRVFCSLGPYANHIVSPETHGTGMPDFKVTE
                     VEIEPTQDPVSSVAMLMGQCGGVPYED"
     gene            complement(22562..23173)
                     /locus_tag="PKM87_04195"
     CDS             complement(22562..23173)
                     /locus_tag="PKM87_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ29301.1"
                     /translation="MPLSIVYYAKEFFRGEWARKFLAAKTAPLVTPTYFRNYPELTGK
                     TCTHHLFCMMACPAPGAIDVVRTPEGWMPRIHVGHCIRCGLCVEACPNGVLRSGRILE
                     SMFYDNTKLSFSFRIDVDTERCMGCGNCAVACPVNKLIDSQMSHGGRASTDDLLLKVN
                     EGKCQVLHVEKCTGCKTCEHHCPNEAIHVARLLEAVHAPEDEL"
     gene            complement(23175..24266)
                     /locus_tag="PKM87_04200"
     CDS             complement(23175..24266)
                     /locus_tag="PKM87_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNQ29302.1"
                     /translation="MTKRTVDVSIPVGPVHPCWKEPARVKCETRGEFVLSAEVELGYM
                     KKGIERIMRGRPWQEVMFLAERVCGICSVIHNMVFIEALEKISAIAVPERAALLRVIV
                     NELDRMQSHILANFSYCYTIEHETLGMYLLDTREKVMDELERITGARVTCAYIVPGGV
                     RFDLRAEDALHLRASLEALEKDITRYMGMFEHGPLIALRSRGIGILTKDAAQEACAVG
                     PTARASGLPESDRRLRHPTYQKLGFTPVSREEGDNFARIMMRFQEVLQSIALIRTCLD
                     TLPEGPIRGGGIPGEGEVAYSGEAPRGELSYFIRTDAAGRVVEISIQTPSIMNIEACC
                     HYMLKGVASLADVPSTFVSSDPCIACTER"
     gene            complement(24263..24715)
                     /locus_tag="PKM87_04205"
     CDS             complement(24263..24715)
                     /locus_tag="PKM87_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29303.1"
                     /translation="MTLLQSLKNLVRSKSIHVAYVDVGSCNGCDIEVLACLAPRYDIE
                     QYGIFVHNNPREADVLLVIGAYTPGWEEKLEGLWEKIPDPKVAIAIGNCPISGCLFNR
                     PHTLVDPPVAKHIPIAAQVPGCPPRPTEILSAILSVAPTVFKDYEVRK"
     gene            complement(24814..25101)
                     /locus_tag="PKM87_04210"
     CDS             complement(24814..25101)
                     /locus_tag="PKM87_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1959 domain-containing protein"
                     /protein_id="HNQ29304.1"
                     /translation="MREPMYEKDLTAMKYAILESRRHDGMVREIAAEIGVPQIRMRRY
                     LMDRFDMLLLENLPARYEQGKKIRENSPEPERQLGSHLYTHAVPIIDREAM"
     gene            complement(25098..25340)
                     /locus_tag="PKM87_04215"
     CDS             complement(25098..25340)
                     /locus_tag="PKM87_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29305.1"
                     /translation="MIREVLVLATLIYLLVIAVQLIDRPALWLQSLAVAAALGLAAGV
                     YWFREELRLGRWEAVALWAAVLLFVAYGLARLGGLV"
     gene            complement(25337..>25542)
                     /locus_tag="PKM87_04220"
     CDS             complement(25337..>25542)
                     /locus_tag="PKM87_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase"
                     /protein_id="HNQ29306.1"
                     /translation="VLLMTFILVFIGSLPFWMILVAMILVTVSLSFVCALSPMLSPYD
                     SVTIQTLIAGLMVAYVALLGVMS"
BASE COUNT         5088 a         7771 c         7146 g         5537 t
ORIGIN      
        1 atgaagtacc tatgaaattg gatgaaagat atattaaaga ctactttccc cagaaagggt
       61 tctcccttcg cctcatcgcg gtgaactcgg caaggacctc ctgggtatga accgggagat
      121 gggggagcat ctccttctcc aggactttta cgtggcgttc cgggatatcc acgagcaggt
      181 catcctccac atcgagctgc ctccccacgg tggcaccctc aacagagatt gcaacctccg
      241 cgccggtctg cgcctcccgg atcgtctcct ggttcagctg gatcgacttc acgtggccca
      301 ccttcctccc gtcaggctgg atgaggttta cgtcagtccg aagcgtccct ccaagcaccc
      361 gcacccctac caccgcgggg ttgctctgtc ggaagatgca gtggggaagg acccgtatct
      421 ttgccgggtg gatgatctgc tcgaaacgct gctgctcggt cttcctcttc agttcgtccc
      481 tccactggat ataatcgtca accagtttgt agatcaccct cccctcaaag accttcacag
      541 gatacccggg ctccttcacc atatcggctg catcaggcag gagggaggtt gagaacgcga
      601 taagcacgcg gaaatacggg tttttgattg tctcggcgtc gatgaggtca tggcggctca
      661 cagaacccac ttccgcccgc agcaccccga tatccttctc gtcgagctcc ttgcagagtg
      721 cctccagggc gccgatggta tcggccttga tgatcactcc ctccggggaa agccggacat
      781 tcacctcctc catctctctc ctgatcctct caatcacctc ttcctggttg cctctcagca
      841 cctggaccgg ggatcccgca atggcacctt caaggttcgg ggcactcacc ttgatccccg
      901 cggcggcagt gaccgacttg acccgctcga accggtcctc tacaagtatc tccttcaagg
      961 gtcggggctg gaggagggca cggacctttg tgaccacgat gccgtcctgc cgggcaaccg
     1021 cgagctcgtc gccgaccgcg agctttccgt catagaggat gacatcgagg gtggtcccaa
     1081 gccctttctc ctcttttact tcgagaatgg tccccatgcc agggccttcc accgagagcg
     1141 agaggtcctg ctccatgtac cgctgcgcaa gccctatcat caccatcagg agctctgcca
     1201 ctccctcccc tgtatgggcg ctgacaggga caatcgcaag gttccggcgg aagtccgaga
     1261 cacggtcgaa ccgttccccc gagaacccga gatccgagag ctcgccaaca atctcataca
     1321 cgcgggtctc caccagttcc ctgaccctgt cattctgctt tgagaaactc gccaggaaac
     1381 cctcgtccct gtgaaccctc cagccgtgaa ggcggtcaat tttcgttgcg gcaatgacaa
     1441 acggggtctt gtaggtgcgg aggatctgga gtgcctcgat cgtctggggc tggaaaccct
     1501 ggttgatatc caccacaaga atcgccatgt ccgccagtgc acctcccctt gcccgcaggg
     1561 tggtgaacgc atggtgaccg ggggtatcga tgaagaggag gcccgggaca ttcagcgcga
     1621 gcttctccat gctcccgctc atcttcctga tggcgtcgat gggaacgatg gttgccccga
     1681 tatgctgcgt gatcgcgcca tcctccgtgc tcacgacaga tgaccccctt atccggtcaa
     1741 ggagcgatgt tttgccgtga tccacgtgac cgagcacgca gacaatgggc gtcctgatat
     1801 gcgtcttctg tgtcattctc ctccacccca ggggtaattc acaccggata tcggcagggc
     1861 ctgcaggcaa agccccgaaa cagaaagcca tggcgggaat ccggaggcat caactgtgca
     1921 atgtaatgta tcaccaggaa ttaaatacac accggcgatc ttttgggtgt gagggttgtg
     1981 aggaggagat atattaaaga gtgccggggt gaaataaagg agggtacatc ctgccagggt
     2041 ggggtagttg gttatcctag gggactgtgg atcccccgac ccgggttcga gtctcggccc
     2101 tggcctaatc acctctattc ggagcctttt cttgattcca gtcataattt aaccatttat
     2161 tgtaactatt cttgaaaagg cacgcaggga gcaaactggt tcttcttcaa taaaagcatt
     2221 ccagtaacag gagaatatga tggagaactg tattctcatc ctactccccc gatgtttctt
     2281 ttctcaatag ccagtatatc cctgacatag gacttaaaat caaagggcaa atatattgag
     2341 aacagcccct gaatcccttt catgagatcc accaccctgc aatacatata cctcctcctt
     2401 gtagcctccc tcattgctgg catgattatc tcttcgggtt gcaccaatac cgaaaagaag
     2461 cccggcagac tcgacggttt tgatgagttt gttaccgcga agatggccga atacgaagta
     2521 cctggtgcgg ttgtcgggat tgtcgagaac gacagtgtgg tgtacctgaa aggttttggt
     2581 gtgcgcgaga ttggcaaacc ggataaggtg gatccggata cacgcttcca gatcgcatca
     2641 gtgtccaaat atatcactgc aggggcgatt gggtccctgg ttgacgaagg aaaactggat
     2701 tgggatgagc cggttgtcac ctatctgcca aattttgcac tgaaagatgc gtatgctggt
     2761 gagcacgcga cactccgtga cctgctcgcc catcgcacgg gccttcgaca tttcgatggc
     2821 gacctgctgg gaaggctcgg atactcaaat gccgagctcc tggagaggat gcgctacctt
     2881 gagcctggca ccagtttccg ggagaggtat atttactcga atgccggttt tttcattgcc
     2941 ggggaagcag cggcaaaagt ggacaatcga agctgggagg acctcacaga tgcacggata
     3001 atccagcaac tcaatatgac acggtcgggt gcccaccctg agacattgta cctcgatgac
     3061 aaccatgtat caggccatgc cggcatgggt ggagatgttc acgttatccc ccttgaacag
     3121 gcctctctgc cggctgccgg ccaggtggtc tccacagggc gggatatgac ccaatggttg
     3181 cggatgatgt taaacgacgg gcgtgtcgat ggaacgcagg tcctcacgcc agagaccgtg
     3241 aaggagattc atgctgcaag cctggttgca ggacatgggg gccctctttc cgatccgaac
     3301 gcagcagtag gccttggctg cgactcgtac aatttccttg acacgcgggt catcgagaag
     3361 aacggggcac ttgacggcgt ccgtgcaatc gtcatcctca ttccagggat gaagaacggc
     3421 atcgtggtga ttgcaaataa acaactgacg gtgtttcccg aggccatcag agatgaattc
     3481 ctcgaacgat acatcgggaa gagcggcgtt gacctgcagg ccaatgagaa aaccaaccaa
     3541 gtagggtgga actctctgat aataactgtc gaacgacctg caaatccagg cccggcgtct
     3601 atcaaccagt cagcaatctt cgggacgtat acgagcgagt tgtatgggac aatgcaggtg
     3661 aaggctggct ctgatgccga gaacatgacg atagagcttg gcccggtcaa gtacccgggc
     3721 actctcatgc atgtaacgaa tgatacttgg tatctctcat tcccaaaccc tgacgaccag
     3781 gttggctatc tgacattcga agtggacccg tctggttctg tcagcggatg tagctcagaa
     3841 gagttcggta tgtttgcacg gttgtaaacg aagggacctg gtttttccct ccagccgtct
     3901 catcgaggga ggagaacccg ggcatatccc aatttatcgg ccgatcaata tccaatttaa
     3961 acagcacgga atagacttat tctcttttgg gttttcctgc ggccccgatc ttccatatat
     4021 ctcaccctga ttacttctct ttctcacact gtaattgtgg aaccttttac tcaccccggg
     4081 accatctgcg cctcgcagcg gggacaatgg aacgtatagc agggagcgac ttggggccgc
     4141 tcgatccgat agccgcagag cgggcagaca cagactgcgc caggcatggt gccaccgcag
     4201 tgcccacccc gcctgcctct gttctggttc atcgaatgta tcacctcccg gcatgtctcg
     4261 atcagacgac cctgatctca tcgctgtcag tatatctctc ttccccttga gtacccgggc
     4321 atgcgtccct ggccagggta ccacccctct cctcaggact gacgaaaaat aagaggcttc
     4381 ccaggaggaa gaggatatcg acaggcggga gcagggatgc aacgagcccg atgactcccg
     4441 cacgctgaat gcacttcttt tccgcaaaat ataccgaaaa tatcgccagc acgagcccgg
     4501 ttatcttgag tgcaagaagg atatacaggt attctgcgac gagatgaatg ggaatcagtc
     4561 tcgaggaggt tgaagcggcc cctgctgaga cgagcaggaa cacaagagcg catccgatca
     4621 cgattcccac cacgaagaca agcttcccgg cctttacctc tgtgctcacc ccgttctcct
     4681 ttggcagttc ggttccctcc ctgtaaccag actcgtttta gacggataaa agtatattca
     4741 ccacgttcgt gcatgcaccg gcagggcgca tgtcccctct accacggtcc cttccatggg
     4801 tggaccagga gcatggattt ccctccatgg gccatatggc aaaaaacccg cgccactgcg
     4861 ggcgaactga tacatttaaa tatcgacact ccccccagat atatatagtt gattggtgga
     4921 gtgtatgcaa cgattctctc ttaaatgtgc gattctctgt gccatcctca tcctttcagt
     4981 ctgctcctgc gtggaggctc aggtggaagg gacggcagat gccgggattt accggtttca
     5041 cggaaatgtc gaaggcgctg aggtgtatgt cgacgatcag atggtaggta caatcaagaa
     5101 cgggctcctc gacctccctg tctctgtttc gggaacccct taccaaacgt ataccttcaa
     5161 gaaagagggc tattacacct acaatggcac catcaatacc gtgcccgcga aaggccaggt
     5221 cttccacatt tacgtatcga tgaccgcgat gccactcgtt gaatacagca gggtgcacct
     5281 gctggtcagc cctcccgatg caaaggttac ctgggacggt gaaccggcag gagatgtgcc
     5341 accgacagga atcctgatca tatacaatgt cgcgcccggc aagcataccg tgaaggttac
     5401 caaagagggg ttcctcccga tcgagcaggc cctgacggtc accgggaacg atgtaatgag
     5461 ggtgccgctg tcacttacgc ctgtgcccct cggttccatc acttttgaat ccgtcccggc
     5521 gggtgcgtct gtctatctcg acggccagtt caggggaatg actccgcttt cactgcatga
     5581 tgttcctgcg ggaagccacg ccgtcatgat taccagtgaa gggttccagg acgtggtctc
     5641 cgcagtcgag gtcacaggag gaaagactac cctggtgagc gagacgctca atcctgggac
     5701 gccggttccc ggaagcacga gggccgcgtt gtcgccaatg gtgctggcgg ggggcctggc
     5761 gattgcaggg ctgctcgtgg catacagaag gccctgactt cctgttactt ttttcctgcc
     5821 tgttctgcct gtattctcct ggcgggttct cctcagtgaa tgcagcaatg tttcttgtga
     5881 gggagacccc gcggattatt ctccctgccg gctgcaggtg gcgttgagat ttcacgtgat
     5941 gagcgagctg acaggagcga tgccgatctt ggccttgcgg atccttgtct cgtcaaggag
     6001 gtggcgcatg tccacgaact ccccctggcc aagtatcttc ttcatctgcc cttccagcga
     6061 tgcgccaggg tcgtatgcga cgttcctgaa taccactcca tgagtatgtg tgatcatccg
     6121 cggccctccc tcattctcct ccccatgcgg ggagaagaat gagttcagga ctgccctgtc
     6181 gaagtgcttc tccgcgagat cgaggtcgac cgcgattcca aaacccacca gggtatcacc
     6241 cctgtacctg gggatcgctc cttgcgcgaa ccgtgagtcc tgcaccgaag ggttcctggt
     6301 ggggatatcc tggctctgca gtccttcgag gactgcccac cacgccactg ccaggactcc
     6361 cctgaactcg aggtgctctt tcttcgccct ctcgatgagg aggcggtaga tttctccaag
     6421 gcgcattccc tcgcgcggga tactctcgag gatgacaaac tcccggaaag gaccctccag
     6481 ggatactgag tacccggaga agatcctgac ctccccggtg tccttcaccg gagtgaagaa
     6541 gtcaggcgtg tcgttgccgt ccgccggcag ccagaccagc gagccgtgga gcgccaccat
     6601 ctctccaagg aacgggagga catcctcggg actttttccg agagccccca ggcccagggc
     6661 atattcgatc ccgctgaagg gtatcaccgc gaggtgcggt gcgtcgatgc gggaattcag
     6721 caccacatcc atgtcacccc atagccggag agccgacggc cgagtggaac cctgctcgat
     6781 acgtaccctg gccccgttca cttcaaagga tggggactgc agatcggcaa gcagggatgc
     6841 acgctcctcc gccttcccga ggacgcgaac ggcctcttct gcggtgggat aatccgggaa
     6901 gatggagaga aaacctgcca tttcaaggac tttcctggga aaatcacccg ttcccgcgag
     6961 agccaccttc ccgccgcgat cccgcagcct cttttgcagg acaagcatga ccctgatccc
     7021 ggcgctggaa aggtagggga cattcttcat gtcgagcaca agtgaacggt cgtcgtcgtg
     7081 cagggcttct tttgcggcct gttccaggac catcgcgccg tgcccgtcca atcttccgcg
     7141 gcacaccagc accaccaccc cgctctcctt cctctctgct atctccatag gggcatcacc
     7201 gatacgctcc agaggtttcg cgggcaacat gatagcattc gtggtttttc cactgccaca
     7261 aactccttct ttatgcccag ctgaagaaaa gagtgagtgc agcatgaaag acccctcgaa
     7321 ggatccgtcc cgcatctgta tcttctccct ctcgaccggg aagctggaac tggtggacag
     7381 ggttgtcctg tcccaagagg agtggcagag gaggttatcg gccgggcaat accgcgttgc
     7441 caggatgaaa gggacagagc ctgccttctc gggagaatac catgaccttc atgatgttgg
     7501 gctctaccga tgcatctgct gcgggacaga cctcttcgct tcatactcaa agtttgattc
     7561 cggtacagga tggccgagtt tcagtgcacc ggtgcatatg aacaatgtcg gttttcatga
     7621 ggaccggagt ttcgggatgg tgaggacaga ggtcacatgc gcccgttgcg atgcgcacct
     7681 cggccacgtc ttcgacgacg gtcctccgcc tgcgaaaacg agatactgca taaattcggc
     7741 atctctctca tttgtcccgg ctcacgggaa aaaccgggaa aagggagaca tggacggccg
     7801 gtgacaaaag agagttccgg tggcaggcgg ggccggggcc gtccaaccgg cactctcttg
     7861 aagacgtccc gcatacctct cctgcaggaa tggtcccgtg aggagagatg catggcagag
     7921 gggcagggca ccatactgac cgccgatgat gcggagttcc tggaggagct ctcggaatac
     7981 ctcgagatcc ttggaaacag cacgcgcctc cgtatcctca ggctccttga gaaaaatccg
     8041 ctcgatgcac gctcggtctc gattgccaca gggatctcct acgagaacac caagaaacac
     8101 ctggagcgtc ttcttcacgc aggtctgatc accaggagcc cggggctcgg gagggagaca
     8161 tcccgcggag tccaccccgt atgggaatat tctcctgtcc ctgacgtcat ccggcgcgtc
     8221 cagcgcgacc tcgggttcct cttcgcgctg ccccctcatg ccgggtggag tggcctgcat
     8281 ggacaggtgt ccacggtcag gcaacgtctc tccctcgaat gggggacgct cgagaagtta
     8341 ctcctcgtaa tggggggcga atgggatggc cggacactcc ctcttgcatc cccctgcact
     8401 gcgctgggaa gagtggagcc tggcgtggat gcgagcctcc cgcgatgcgg atgcatctct
     8461 tttcccgagt cttacaggtc gctgacacgg atctcgaggc ctcatgcact gatcaccgag
     8521 cgggacggga ggtgtgttct cgaggaccgc ggcagtaccg gaggcacgta tcttaacggg
     8581 aagaggattg cggagcgtca acaggtgctt ctcagggacg gcgaccgcat ctcgctctca
     8641 ctcggggagt tcggcgcaac tctcctcttt ctgcataagc ctgatcagga cactggtgaa
     8701 ccgggggcag ggatgtgaag catcctctca ttattctcac ggtgctcctt gcaggaatga
     8761 tggtatgcag ccaattctgc tcggcatgga tcacctacga gagcaacgct tcacggggca
     8821 tcccgtcgga actggttgag aagaacaagg caatccagga gaagtacaag gagattgcag
     8881 agagcggtct tccccagttt ataaagccca ccaatccatt cccgctgccc gcaacgactg
     8941 tccccaccgt ggtcgtcacc tctgccatcc ctacagatta cccgacccct gttcccacca
     9001 cggcatcgcc agagcccacc accaacgtca cacccatcgc atccggcgat caggaagagc
     9061 cggcaacgac tgccgctttt ttttccgctg tcctcgtgct cgtgcttgca acgatcagcg
     9121 ccatcctgaa ccttgccgtt cgatggaggg accgcgaacc ccccggactt gcggccccat
     9181 ggtggagccg ctggcacggg gtaattgccg gggttcatgc actgatggcg gtgatcttct
     9241 tccttgaagg cctggtcttc atcgattccg ggatgctctg gttcctctct tccggaaggg
     9301 agattgcatg gatcatcctt ggtgggttcc tgcttgtcaa cggagccgtt tcatcggcgg
     9361 gattggccat ctcttccttt tttcggagag agagaaggaa tgtctcgatc gttcatgctg
     9421 cactcctggc cgcaggtctt gcggtgtatg cagtattcct ctttgccccc atctttccct
     9481 tccctcaagc cgttcccgtc gccgcgttcc tcggtggcct cggcatcata cttatccagg
     9541 caaatgttcc acgggagact gcggcagaac cgccatcagc ctacaccgac accatgctct
     9601 tcgaggacaa ccctgcggct gccacgcctt tcttcccccc cgagctctcc gggaggtact
     9661 ttgacgcacg gttcctgcac cagggtggaa tcgcgcgcgt cttttctgcc aggcgcagga
     9721 gcgatggcac aatggtggcc gtgaaagtgc ccattcatac ggatgagcag acaggaagat
     9781 ccctgcttcg cgagatgagc gtctggaaga cacttatcca ccccggaatc gtgcaggtca
     9841 acgctgccaa catccttccc gtcccgttcg tggagatgga atatctcccg ggaagcctcg
     9901 aagacatccc gctccccatg gaacccggtg atgctgcagc cctcgtcagg aaggtcgcag
     9961 aggctctttt gttcgcgcac agcggcggcg tgatccaccg cgacctcaag ccaggaaaca
    10021 tcctcctcac tgccaggggg gagccgaaga tcggagactg ggggctctca cgcaatgacg
    10081 gcatcccttc ggagaccacc ctgcacgggt tctcactctc ctatgccgct cccgagcagc
    10141 ttgaccccgg aaggttcggc cggagcaccg ggaagaccga tatctaccag atcgggatca
    10201 ttttttacca gctcctcaca ggcacgctcc ccttcccagg agagagtatt gcggaagtga
    10261 caacggagcg gctttccggg ataagcaaac cattctttgg caacaatcct tccctttcca
    10321 tgttcgggcc tgtcatcggg aggtgcctgg aaacaagccc cgatgaacgt tattcctctc
    10381 taaaagatct cctctccgac cttgaggctg cagaaaggaa gtaccgggca ctcgcaggga
    10441 agtcccccgg ccatgaagag gaggattttg gcagtggttg agcaccccgg tgacgcagaa
    10501 gaaccccatt tccggcatcc tttccggcaa gacgggagag ccagggtccc tgtcgcactt
    10561 gtgaggtgcg ccgagtacca gccggaccag gtctttcgtg cgctcaatga ggctattaca
    10621 cttgcgggag gcatcgactc gtttgtcact cctggcacac gcgtgctggt caagcccaac
    10681 cttctgcagg ggctcccacc ggaacgtgcc gtgtgcaccc accctgtcgt ggtcggggca
    10741 gtgatccggc tgctgaaaga gcacggctgc acggtcaccc tcgccgacag ccccggggcc
    10801 ggcatccgtt acacgccgac gtcgctggcg cgccagtacg agcgggcagg atatgagggg
    10861 ctcgctctgg atcccgggtg cgtcctgaac cttgatgcgg actctcgcct ggtgaagtat
    10921 cctgagggga aggtatgccg ggagtttttc ctcatttcac ccttcttctc ctctgatcac
    10981 gttgtcgtgg tctcgaaagc caagacgcat gtcctcaccc tcctctccgg ggccaccaag
    11041 aacgtctttg ggctcgtacc gggactggag aaaccgctcc tccactccag gttccagacc
    11101 ggggagagat tcggggatat gcttgtcgac ctgaacctgc tggtgcgtcc ttccctgcag
    11161 gtcgcggatg ccatcgtggc aatggaagga gacggaccga cgtcaggctc cccccggcat
    11221 cttgggtttc tccttgccag tccaagccct ctggcgctcg acatggtcct gtgcaggctg
    11281 atgtcgcttg atccccttga cgtcccctac ctgaagtgtg ctatcgttcg cggactcatc
    11341 gccccggacg gacgcgacat cgaactcctc ggcgacccgc tgcctccatc catggaacac
    11401 cctgtcagga agccagggac ctacatcaac cacagcggaa gcatgccagt cacccccctc
    11461 ttccgggcgg tccacacgct ggggagcctg tgtgcaatgc ggcccatagt cacgcatcag
    11521 aaatgtaccg gctgcgcaaa gtgcgcaaca atctgccctg cccgggccat cactgtctcc
    11581 gggggacgcg cccgcatcag cctccaccgg tgcatacgct gttactgctg ccacgagatg
    11641 tgcagcgaag gcgccatctc gctggagaga agtcttgccg gatcgctcct gtacagggtc
    11701 atgcaggcct gcggatgggc gcagggcaaa aagggtcacc ccctgtcttg aaccacgggt
    11761 atccagctaa tttttaacca ggcctcctcc actcgtgtag cggagcaatc tgatgagaga
    11821 gaaggtcatg atgatactgg caatcacagt atttgccgct gtacccatgg tacagctcgt
    11881 cgtcccaggc tgagtggtga acatgggtgg gaaaggatcg cctttcaata ctcacccgga
    11941 accaggattt ttttttctta taacaatccg caaccactct ataggcagac agggaatatc
    12001 cttctcatga tggcagtcct ccctcatacc ccgaggcttt tggaaagata caggtatgga
    12061 gacatcgcga ccggaactct cttttacgcc cgtaaagcct ggaagagggc aattgccgta
    12121 aacaagtggc cccggtgatc acgggcgcgt tacatgattg tcagattggt ctttctccac
    12181 atacccactc attcatcgcc tatggctgca aatggggaga tgatatgaga cagaggacat
    12241 ttacaacggt cattatctcc ctgactatcc tggcactctc gtgttcggca ttgtgcggag
    12301 cctgcgctgg aggctctatc acggcaacaa tcggggattc tatccccctg aatggcaccg
    12361 tgcagctcgc tgacacggtc tatctcttcg tgacaggtcc agggatgcct ccaaacggcg
    12421 cccggatgga gaacagcaat gcggctgtca tcaccgggga tccggacacc ttcacccagg
    12481 tcatggtcga gaacgaccgg tgggcttaca cgtggaacac gggacgggtc tcagggggac
    12541 ttgccccggg aagccatacc gtgttcgtct cgacacagcc tgcggctgca aatgcactct
    12601 ccggcgtgaa ctatgccgag atcgaggtta tactccatgc cgcggttacg accgcgaccc
    12661 tcgaagttga atcgagtcct gcagacgctg cgatatactt aaacgggaag tactcaggca
    12721 acagccctcg cgtattcaga gaccttgccc caggggagta tgcgatccgg ctggaaaagc
    12781 agggatatac agatgagacg gggtcggtca ccctggcagc aggagacgac gtgaagttca
    12841 cacgcacgct ctcgccggaa ggaacacctg ataccacggt ggcgacactg cccgccacat
    12901 tcccgaaagt gggggagact ccgcccccgg caccgaccac cacgcctctc acttcagcct
    12961 gcatcgccgg aacgctggtc cttgccggac ttttcttcag gaagcctgat tgatccctgc
    13021 atcttctttt cccggcgcgg gatgttctgg gaacctccgg ccctgacagt catgcatacc
    13081 ctgaggattc aaggtgattt ttcccgatcg agccccgaag agttaacctt atatctcctc
    13141 ctccccatcc cttctggtga ggtgaaacga gcgtgtgcac agttggcggc cccgttgaca
    13201 tggacctccc cccggaacgg gtgaagggca agaattatcg gtgcaaagac tgcggggaga
    13261 agttcaaggg agtgggcagg cacccgatgt gtccttcctg ccagtcagag gacgtggaag
    13321 aggtataacc ggagatccgg agaatagatc tccccacaat caattgtcca tccatttcca
    13381 ggcagatacc tgactgcgag gggatgtccc gaatggagag gcaaatcaaa gaatttttca
    13441 ggatcttttt tctttttcag gggaagccat agcgtgggtt atatattgaa gcacctgctg
    13501 aaggggagat cgctcatttt tttaagcctg gggacataaa aggtcccatg agactacagc
    13561 caatctttct cgtcgtcctc gtgtgcggca tgatctttat caccggctgc acgcagcagg
    13621 aggtgcaggt cactcccaca ccaaccccca caccagaagc tccaccccct gatatcagcc
    13681 cgttaccgac cgacacagtg cccacagaat acagagtatt cgtgacagtg acaaggaata
    13741 cctactcctt tcaccctctc atcaaggttg aattcagggg ggggcagggg atgggcctcc
    13801 tctcttcgct tgatgcagag gtcatccggg aagacggctc tgcagggaca gccagccttc
    13861 agagaccggc gatcggagag ttcatggaag tgcaaggctc gaccggcaag gatcgggtaa
    13921 tcgtccatgc acacatgatg aacggcgccc ggtatcgtat ttatgacgat gtcctggagt
    13981 tcaggacgta aaatcaactt cttttccaga ttttttttga acctgaaatc cgccgacccg
    14041 ttacggggac gcggaacgcc ggaatgcacc atgcggaaca cgtatctcaa accgcgcccc
    14101 tttccccggt tcgccggtct cccttatcga gaatcccgtg attgcgagca cctcgcggac
    14161 aaggaacaac ccgtaccccg tgttctggcc cacgccaagc gagaaaacgg cctctttctg
    14221 ttcggcggga atcccggtcc cgtcgtcctc gcagacgaga aggagcccgc cctcgtcctc
    14281 ctgcgatgag aatgtgatcc gcgtgaggct tcctccgtac cggacagcat tctctatcag
    14341 ggagaagaag accttctcga gcatcgggtc cgcatagatc tcgagccctt cggtatccag
    14401 gtagatggtg acatggacga gatcctgcag ttcggcacct ctcctgactg ccgcactcac
    14461 gtcctgccat gagggggatt tcattcccac gttctggtag ctctcggtga acgcaaggag
    14521 ggcctgcagc gcctcgatac cccgcttctc cttcctgatg tacgtgatga gaaggggatc
    14581 atctgtctgc tcttccatca gttcgaggta gcttgccagg acggcaatcc tgttgagggc
    14641 atcgtggcgg gtgatgcggg agaggagtga gagcttcttg ttgagaagag agagaacttc
    14701 ctgggccttc ttctggtcgg tgatatcaac ggaaaatacc gctgcgcggg ttaccttgcc
    14761 aatcgaatcc cgcacaggat agacatttgc aagagagacg cgatcttttt gggcaatctc
    14821 gaaacgtatc tcctttcccg tggagaagac atcgcccacg cgagcctgca ttgttcctcc
    14881 catctccccg ttgaccaggg tgtaggcatt catccccgct accgcagtat ccggtaccga
    14941 ataacgagaa cagaacgccg cattcgcaag gataatccgg ccctcggcat caatgaggca
    15001 tgcgggttca gggatagcgt tgatgaacgt gcggagcagg tgttcgctct ctgtcagtgc
    15061 ctcctctgct ttctggagat cagtgatgtc gaatgcgatt ccaagggtcc cgatgatatg
    15121 cccgtcattg cccttgagcg gctccagatg ggccttatag accctgtccc ggtaataagc
    15181 ggtataactc accgattcac cattgagcgc tctcctgtgt gcagaaaccg cttcgagcgc
    15241 ccccgggctg ttgccatgga atgcctccac cttcattccc accacctggt tggtcagaag
    15301 gcccatgtcg ccaagccccg caccatagga tgaagtgaat acgaggtcgg tatcggtggt
    15361 ccagagagat ccggggacct gctttgcgat catttgcagc cgctcttccc tctccctcaa
    15421 ctcgtcctca gccttcttct tctccgtgat atcaagggcg attccacggg tgccgacgat
    15481 acgcccgtgt tcgtccctca tcacgctcac attgttgatc accgggaaca cacggccctc
    15541 ccccctccgg gcgacgaagc ggcactcaaa attcatcaca ggctccccgg tctggaggat
    15601 tccctggaat atcgcaatcc ctgcgcggat atcttcgggg tgcaggttct gttcgaacgt
    15661 ctggccaatc atctcctcct ccgtgacctc gaatgccccg agcccggcct ggttcatgaa
    15721 ggtgatatgc ccttcaaggt ccgcagtgta gaggatatcg ggaatatcgt ccacgagttc
    15781 ccgatacttt cgttccattg cgcgggccgc agcctcggct cttttctggt cggtgatatc
    15841 cgagaccgtg aacacaatgg gcgagtgtcg gtcgacggga ttgaggggcg aggaactgat
    15901 aaggacgtca aagacagtgc cgtccttcct gacccaccgg ctctggacgg acccaacccc
    15961 ggtcttccac atgggggggt acttctcctc gccgaccctc acgaactcct cgtcgctctc
    16021 gtagagtatc cgggcgcttc tccccgccag ttcttccgga gagtagccgg tcatcctggc
    16081 aacctgtgca ttgacccagg agaggacacg gtcctgcacg atgccgatgc ccacgggagc
    16141 cgcccggaag atgccctgga ggagcgcctc gcgctcacgg agttcttcgc ccgccctcct
    16201 ctgctcgttg atatccgtga tagtgccggt gcaaccagaa tatcgctccc catcccacag
    16261 ggtgcgaagg gatatcctga cccatcgctc gtcgcccacc tttgtcttca ccagccattc
    16321 tacagaaaat ggctttgaat tacgctcctt ttgcagcagg gcaagtcctt ccatgacttc
    16381 ctggatatct gaggggtgca ggtattcagt gaaccggtga cccgtcacct cctccggcag
    16441 gtacccgagg atacggacca cagccgggct cgcataagtc acaactccat ccgaatcgag
    16501 cgagaagacg atgtcattga tgctctccac gagctcgcgg tatttctcct cgctcttcag
    16561 gagggcctcc tccgcctttt tccgcgaggt gatatctatc gtctgtatca ggaacccctg
    16621 aattgccccc tgcggatcgc ggagcggttc gagagaactt gaggcatacc acacttcacc
    16681 tcctttcttc tgcgaacgga actccaggtt ccgtaccccc ctgagttccc tgaaggcggt
    16741 ggagatatcg tcctttaccc gttccctgtc ttcggggtgg acgagcgaga gcgcctgtcc
    16801 ttcgaattct tcgggcaggt accccgtgaa atcgcggacg ttcgggcttg catacgtgat
    16861 tctgcccgaa agatcggtga tgatgagcat gcttggcgcc gtctccacca tcgtcctgaa
    16921 cttctcctcg ctgcggcgaa gcgctgcctc ttcctgctcg aggcgccggc agtagtcgtt
    16981 catcgagtcc tgggtggccc ggagctcctc gagcgttgcc gccatctggt tattggaggc
    17041 ctcgagctcc tctttcgcct gcatcaatgg agagatatcg tgtatgaggg ccacaaaata
    17101 gtccaattcc ccgttctctt tcctgacagg gagagaagag acctccacgt cgatgagccg
    17161 tccgtctttc ctgatatacc gtttcttgac cgtgtgtgat tcgctttcac cggaaacgac
    17221 tcgcgtgtag agctcgagct ccttcgcaag ctcttccgga ggggtgagat ctgaccactt
    17281 gagcgctttc aactcatcaa ggctgtaccc gagcatatgg cagagcttct ggtttgcgtt
    17341 gatccactgc atgtcgggtg acgtgatggc aattcccacc agcggcaggt cgaagtagga
    17401 ccgaaacttc ttctcgctct ccacaagctc ggcctctatc cgctttcgct tgaggatctg
    17461 ccacaatccc cccatcaggt gcgtgacctg ccggatatca tcttcgttgt actcggagcg
    17521 cttgttcccg aaccctgcaa ccatcacgat ccttttaccg tccatcacag ggacgccgag
    17581 gtgccggatc atcttcacgt ggccttccgg gtgccccttc tttgccgggt ttggcgccgc
    17641 gtagtcgttc gtgatcaccg ggcgcctctg gcggaccggc tccccccaga ggcctgtctt
    17701 ctcgacagga tatatgacct gtttttctgc aacacggcac tcggagagcg cggtcttcga
    17761 ccaggcgtac atggagagca ctgtctcttc ttcattcaga aacgcaatgt agcccatcgt
    17821 gcttcccgtg agagagattg ctgtctccag ggcataggtg cagatatccc gtttatgtgc
    17881 ggccgacatc tggtggagat cgatcatcgc ctggagcctg tgctggttta agcgcacctc
    17941 ctcctccgcc ttctttcgct gcaccgcctg cacgatcatg ttgcgaagct cggcaaactg
    18001 ggacttcggg tcaccccctt tctggaggta gaacgaggcg ccgatgttca gcgcctcgat
    18061 ggcgacatgc tccctcccgc ggcctgtgaa gatgatgaat gggatatcga cgcctcttgc
    18121 cttcactgcc tttaaaaaat cgatcccgtt catctgcggc atctcgtaat cagagacaat
    18181 gatgtcgaac ctttcagtat ccagcatgcg gagggcggat cccgccgatt ccgagagggt
    18241 cacagacatc tttccatctt tttcgaggaa tatctgggtg atatccagga gagctggttc
    18301 gtcgtcaacg aggagtacgg agatcatggg gtatcacggt ggaattacag gagatacact
    18361 aaaaaagatg catttccagg agtaaaagga tatgggaggg attgtgccgc atggtatctc
    18421 ggacctcttc aggaagtgtc atccccggac catcagggtt cccttccccc tgttcgcgac
    18481 atctcccctg aagacgttca tccggtatcg cttgcccgcg tactctgctt cctgggaccc
    18541 gttcctgcgg aatgtcggga ggaactgccg ggcttgtcca aagaccatgc acgtcccgcc
    18601 ggtcatgttc ccgcatggcc gcaggcagtc ccccccgatg acaagcgtcc cgccccgcat
    18661 ctccacgccc ggcatgtcgc cgcagttccc gtggacgata acctcgccgc ccgagaggta
    18721 ctcggcgcaa aaatcaccgg cgttcccctt cacctccacg gtgccgcctc tcatgccacg
    18781 cttttccccg cggtatccgg ccgcacagta atggccggca ttcccctcgc aggatatgcg
    18841 cccgccccgc atctcgcgcc cgagccacgc gtcggcattt ccccggatga ggatctcccc
    18901 ggcgctcatg aagttgccgc agtgcatccc aatatccccc tcgatgatga tcctcccggc
    18961 gttcatgtac tcgcccaccc tcttgatcac ccctgtgtca ccggtgagga tcacctctac
    19021 ctggtccggg gagaccgcag ggccctctct ggtaacaaag aagaggtcat caagggaccg
    19081 ttccttgttc ccctcgaaga tctttatgtc gttttttcgg aggaagttct ggggtattat
    19141 ctcctctgcc tcgatgggga ttcttggttt cgcacggggc ttggtcttca ggatgaccct
    19201 catagtgacg cctccgtatc gatccgaagg cccctcggca ggtagacgtc ctggaccggg
    19261 taattgcgca tcctgaccgt atagtactgc tcaaacttct tcgtgaactc cgggtcggtc
    19321 cccatgtcgt attcgggcgg gacgtgtgcc tttgaccaga tggtgctgtt tgccccttcc
    19381 actatcacct cgccgtcgcg tgccactacc cggccgcgct ttatggtgaa gcgggagcgg
    19441 gagaacgcgg ccatcacctg ctggtaatcg actgaagggt ccacctggtc cggactgatc
    19501 gggtagatgg cgacatcggc ctcggctccc ggcgccaggt atcccttccc gatacgggtg
    19561 attccaagcg cctttgcctg tgcggcgcgg gtcatcactg caatctcgta ccaatccagt
    19621 tctctctcga tagcgaacag gggcacgaaa tgctcggtcc tcgggtcgat catcttccgc
    19681 tcgctctcgc gcagcctttg gctcatgagg agcgcgatga tctcggggta tttcacgaac
    19741 ggtgcaccat tcgggctgtc ggtggagagc aggcactgcc aggggttctt cgcgaggagt
    19801 gcaagctcga gtcctatgga ccacatgaca cagttgacga ggctctttct cccgtagtag
    19861 acagggatga tcccggaccc ggtctcgagc tcgacgtcgt gattgctcca cttgttatgg
    19921 tggagcataa agagcttgaa ctccataggg ccatccgcgg tcatggtggt ggtcctcccg
    19981 aacatgatct gccccatgtc catgaccacc tgcggcttgc cgttcaccgc acgggcgatg
    20041 tcttcagacc gtgagcagat gtccctccat gtggaccctc cataggagtg gaactggaca
    20101 tgcgtgaggt acaggctctg gcgttccttg ttcaggtcag ggaccaggtc gatcgtgtcg
    20161 agggtggtcc ggaagttccc gggcaccccc aggttgttgc agtggaggtg gaccgagtgc
    20221 ggcaggttca gcatctcgtt tgccctgatc atcgtcccga tgatctcggc aggcgtcact
    20281 tcgaagtggg ggaccaggtc ctggatgctg tgtacatcct cgccccagcc ccacgcctcg
    20341 gtgccccctg ggttcgtcag tttgatggca aatcccctga ctgccgcaag cgtccatgcc
    20401 acgatggcgg ccgccctctt cacgtccttc tccctgaccg cttccatgat ggaccagttg
    20461 ccatcaaaga gcgtgttggc aagcatgtcc tggtgaggag tcgcggtgaa ctcctcgtgg
    20521 gtatgcctcg cctcaagagg tgccatcgcc ccctcgagga ctgtcgtgta ccccatggtg
    20581 ctgtaacggt aggagttgcc gaacgtcgtg ggcacgctgt aaccggatgt cacgtgcatg
    20641 acgcccttcc gcgcggtcct ccctgcccgc atgtcctccg ggctcatgta cctgccgaag
    20701 ttcaccttgg tgccgcagac gtgggaatgg gagtcgatcc cgccagggag ggtgaggcat
    20761 ccggctgcgt ctatgacctc cgcgtctcgg ggtgcctcgt cgacaatcct cccgttactg
    20821 atcgcgatat ccatgatctc tcctttcacg cccgagagtg ggtcgatcac gaacgcattc
    20881 ttaatcagga gccctggcgt catccctttg cctccttcac gatggcataa atcttctcaa
    20941 gcacctcaag gtcgtcagga tatcttccct ggaagagctt ccgggtccgg atcgggacgc
    21001 cgtccatccg gtaggcagtc ccttcggcat cgatacccgc gaccgcgacc gggatctgaa
    21061 tgcgcgagag gtgggtggtg acgttgacat gggggtcaat ttggaccgtg gggatgcgtg
    21121 cgaggtgctc tgcgcatgcc cgcgggaaat gtgcgccggg gtcgcttgcg acgacaaggc
    21181 aggcatcgca ctctcctctt cggaggatgt ccacgctcgt ggtctccccg ggattgtaga
    21241 atgcaatccc cctcgcaaaa tcgaccgcga agggataccc ggtgatccat gtgaacactt
    21301 cattcgaacc atacacgttc cagtgccccc tcatcgggga gatggtgaac tttgtgtgcc
    21361 ggttcagctc ctcagtcagc tcgatggcgt tccggatgtt tttattcttc ccaggagcca
    21421 tcgtgagtcc cacaccaaag aaaaaggccc cgaacttggc tgctatgcac atatcggcaa
    21481 ccctgaagag ctgctcgcgg gagactccct cgacatcagg cgggatgata tcgcgcttgc
    21541 ccctgacgat cgcccggagt gccgagagga ccgcgtagtc gcccccgggg tttaccttca
    21601 caaactcgtc tgcgacctga gaggactcgg tctctcggac gtccacgacc accaccttcc
    21661 tctccctgaa tgcgttcttc acaaagaacc cgtcgcaata ggtggtgtac ctgctcatgt
    21721 gcctgggatg tgcgtcgacc gggtttgcac cccagtatac caccacgtcc gcccggttct
    21781 tgacctgccc gagagtgcag ccggggtggc cgacctcctg gatcgccatg atggacgggc
    21841 cgtggcatac cgtcgaggtg ttgtcgatga ccgcgccgag gacctctgcc aggtgaaccc
    21901 cgatggtctg ggcctctccg tacgtcccgc tccatccgta gaggagcgga cggtcggcgt
    21961 caaggaggat gccggcggtc tccgcgatcg cctcctcgta ggagaccttc tcccacttcc
    22021 cccctgacct tcttatcggg gcctccagcc ggtggtcccc catgaacttc tcattcgcga
    22081 gcgtgcagcc attttcgacg ttgataacgc gggttccttc aacctctacg acgaggtcgt
    22141 cgcacaggca cccgcaaaac gagcagacca cgtcctcaat cctcatacgg gacacccccg
    22201 cactgcccca tcagcatcgc gaccgaggat accgggtctt gcgtcggctc gatctccacc
    22261 tcggtgacct tgaaatccgg catcccggtg ccgtgggtct ccgggctgac gatgtggttg
    22321 gcgtacggcc cgagactgca aaagaccctc ccacggacaa gtccgggatc ggctcttgcc
    22381 ttcatcacga cctctccggt gctgctcttc accctcacgc ggtcgccctc ctccaccatc
    22441 atctcgaaca tgtccacggg gttcatgcgg atcgctgatg cctcttcccg gtacgaaggg
    22501 gcgttctttc gctcaacata cgatccctgc cggatcgtcc tgcccgtgtt gaagaggaac
    22561 ttcatagttc gtcctccggc gcatgaaccg cctcgaggag gcgggcaacg tggatcgctt
    22621 cgttcgggca gtggtgctcg caggtcttgc accccgtgca cttctccacg tggagcacct
    22681 ggcacttccc ctcgttcacc ttaaggagga ggtcatcggt ggatgcgcga ccaccgtgag
    22741 acatctgtga atcgatcagc ttgttcaccg ggcaggccac tgcgcagttc ccgcacccca
    22801 tgcacctctc ggtgtccacg tcaatccgga acgagaacga gagtttggtg ttgtcgtaga
    22861 acatcgactc gaggatccgg ccgcttctta gcaccccgtt cgggcacgcc tcgacgcaga
    22921 ggccgcacct gatgcagtgg ccgacatgga tgcggggcat ccacccttca ggcgtcctga
    22981 cgacgtcgat tgcgcccggg gcgggacatg ccatcatgca gaacaggtgg tgagtgcagg
    23041 tctttccggt aagctcgggg tagttcctga aatatgtggg ggtgacgagc ggagcggtct
    23101 tcgcagcgag gaacttcctt gcccactcgc cacgaaagaa ctcctttgcg tagtagacga
    23161 tcgagagcgg catgtcaccg ctccgtgcag gcgatacagg ggtcagacga cacgaacgtc
    23221 gaggggacgt ccgcaagcga cgcgaccccc ttcagcatgt agtggcagca cgcctcgatg
    23281 ttcatgatgg agggggtctg gatggagatc tcgacgaccc tcccggcagc atcggtcctg
    23341 atgaagtagg agagctctcc ccgtggagct tcgcccgagt aggcgacctc tccctcgccc
    23401 ggtatccccc ctccccggat cggtccttcc gggagcgtgt ccaggcacgt gcggatcagg
    23461 gcaatactct ggagcacctc ctggaacctc atcatgatcc ttgcaaagtt atcgccttcc
    23521 tcgcgggaca cgggggtaaa gccaagcttc tggtaggttg ggtgccggag ccgccggtcg
    23581 ctctccggaa ggccgcttgc ccgggcagtg gggccgacgg cgcaggcctc ctgggcggcg
    23641 tcctttgtca ggatgccgat cccccggctc ctgagggcga tgagggggcc gtgctcgaac
    23701 atccccatgt accgggtgat gtccttttca agcgcctcca ggctcgccct gaggtggagc
    23761 gcatcctcgg cacgaaggtc gaaccgcacc ccgcccggga cgatgtaggc gcaggtgacc
    23821 cgggcaccag tgatcctctc gagctcgtcc atgaccttct ccctggtgtc aagaaggtac
    23881 atcccgagcg tctcgtgctc gatggtgtag cagtatgaga aattggcgag gatgtggctc
    23941 tgcatccggt cgagctcgtt tacgatcacg cgcaggagcg ccgcccgctc gggcaccgcg
    24001 atggcgctga tcttctcgag cgcctctata aagaccatgt tatggatgac cgagcagatc
    24061 ccacagaccc gctctgccaa aaacatcact tcctgccagg gcctcccccg catgatccgc
    24121 tcgatcccct tcttcatgta gccaagctcg acctcggcag agaggacgaa ctcgccgcgg
    24181 gtctcgcact tgacacgggc cggctccttc cagcagggat ggaccgggcc gaccgggatg
    24241 gagacgtcga ctgtccgctt cgtcacttcc tcacctcgta gtccttgaac accgtaggtg
    24301 cgaccgagag tattgccgag aggatctcgg ttggcctcgg cgggcagccg gggacctgtg
    24361 cggcaatcgg gatatgcttt gcgaccggag ggtcgacgag ggtgtgcggg cggttgaaga
    24421 ggcaccccga tatggggcag ttcccgatgg caattgccac cttcgggtcc gggatcttct
    24481 cccagagacc ctcgagcttc tcctcccatc cgggagtgta ggcgccgatt accaggagga
    24541 catctgcttc ccgcgggttg ttgtgcacga agatcccgta ctgctcgata tcgtaccgcg
    24601 gggccaggca tgccaggacc tcgatatcgc agccgttgca ggacccgacg tcgacgtacg
    24661 ccacgtggat ggattttgac cggacgaggt ttttcaggga ctggagcagg gtcatgcggt
    24721 gatctccttt cgcatcctct cccttccgga atggatggcc tggtcgaggg gagtcccctt
    24781 gcggaccatc tccctgacgc tttcgagtat ctgctacatc gcctccctgt cgataatcgg
    24841 aacggcatgc gtgtagaggt gtgatcccag ctgccgctcg ggttcgggtg agttctccct
    24901 tatcttcttc ccctgctcgt accgtgcggg caggttctcg aggaggagca tgtcgaagcg
    24961 gtccatgagg tagcgccgca tccgaatctg ggggacgcca atctcggcgg caatctcccg
    25021 caccatcccg tcgtggcgcc ggctctcgag tattgcgtat ttcatggcgg tgaggtcttt
    25081 ctcgtacatc ggctccctca taccaggcct cccagccttg caaggccgta tgcgacaaag
    25141 aggaggaccg cggcccacag cgcgaccgct tcccacctcc caagacggag ttcctcgcgg
    25201 aaccagtaga cccctgctgc caggccgaga gcggcggcga ctgcaaggct ctgaagccag
    25261 agggccggcc tgtctatcaa ctgcacggca attaccagga gatagatcag ggtggcaagc
    25321 accaatactt ctctgatcat gacatcaccc cgaggagggc aacgtatgcc accatgagcc
    25381 cggcgatcag cgtctggatt gtgacgctgt cgtacggcga gagcatgggg gagagggcgc
    25441 atacgaacga gagggagacg gtaaccagga tcattgcaac gaggatcatc cagaaaggaa
    25501 ggcttccgat gaagacgagg atgaatgtca tgaggaggac at
//
LOCUS       DAOQYR010000021        24942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_2325_length_24942_cov_9.028288, whole genome shotgun sequence.
ACCESSION   DAOQYR010000021 DAOQYR010000000
VERSION     DAOQYR010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 24942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24942
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2325_length_24942_cov_9.028288"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="PKM87_04225"
     CDS             <1..890
                     /locus_tag="PKM87_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNQ29307.1"
                     /translation="FLFTPDFSVLSDPLLWSAAFGQAFFSLSVGTGILLTYGAYMERG
                     MHIPRSALIITLSDIAVAILAGIVIFPLVFSFGLTPAAGAELAFTTLPMAFAAMPGGV
                     FFAVSFFLVLFFAALTSSVSMLEVSVSSLQEAAGWSRKKATLLLGILMAAVGLPSALS
                     YSSFHLTFNGIKVLDFMDETVGTLGLPIAGILLAVAFTWFVPRDVLSREIGTALSRVV
                     HPLCRFVIPSVLVVTTVARLLAGIDLADLRLLPGSRWIGTVLQAEGMAAIIVIVLLLI
                     MLLCRVVSCPLGAWLLGKR"
     gene            complement(918..1607)
                     /locus_tag="PKM87_04230"
     CDS             complement(918..1607)
                     /locus_tag="PKM87_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HNQ29308.1"
                     /translation="MPFTGGISLFTSGKDDSLTFTVLRLLFPVLLAFTYFFSSLLVLP
                     PEKSLILGGLMIVYYIPPAGKESIIPLGIGLGIPWWLMAASLAFLDVITSLFVILNFR
                     IALRIPVLGPWISRFLASGDAFMEKHPWLSRWSVLGVAGFVLLPLQGTGGVGATLVGM
                     MAGLSPGKILLAIGIGASAECLIFALGSELIWRLIVENLVLGLGVALAVIIAGIILYL
                     AFRRRFRELHG"
     gene            1881..2336
                     /locus_tag="PKM87_04235"
     CDS             1881..2336
                     /locus_tag="PKM87_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2178 domain-containing protein"
                     /protein_id="HNQ29309.1"
                     /translation="MKQNTFFLLAGIVGLVEVGLFWLSVVMRNPLIITAVFIMGVGLL
                     YVARMTITDQREDERAVLITQKAGSRTLEVFWVLFFAVSLGSAVIGFSTPLGLPRPRE
                     LPHDFPRDEPHLGYFGILQMILLCCIAFLYIGFRVYYARKYGEWETDEE"
     gene            2326..2517
                     /locus_tag="PKM87_04240"
     CDS             2326..2517
                     /locus_tag="PKM87_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNQ29310.1"
                     /translation="MKNKIKVFRAMHDLTQEALAQKLNVTRQTILAIEKGKYDPSLEL
                     AFKIARFFGVSVEEVFIYD"
     gene            2723..3412
                     /locus_tag="PKM87_04245"
     CDS             2723..3412
                     /locus_tag="PKM87_04245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HNQ29311.1"
                     /translation="MTATSLFILQHEKAEPAGIFEEVARTRGISSTVVPLFETHEVPP
                     VTSTHLLVLGGSMSVNDGARYPFIDQEKRLIRSWIAKGRPVLGICLGAQMIAAACGGE
                     ISPCRKEIGWVPLEVIDIAVLPGVPRSFMAFQLHGETFSLPAGADLVCRGKEVPHQAL
                     AVGSALGLQFHLEPTEQMIRLWLSEFHPGEQSRILQDTARYLAESHRLCRIVCDRFFQ
                     AAPRGFCWRCP"
     gene            complement(3462..3854)
                     /locus_tag="PKM87_04250"
     CDS             complement(3462..3854)
                     /locus_tag="PKM87_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HNQ29312.1"
                     /translation="MEDAEVFFGKDAYARHTGIELLEIGPGTARVRMEIREMHENSHG
                     TVHGGAIYTLADAAFALASNSHGIPAAAINASISYMKSARGGSLYAEAREFALNPKLA
                     TYTVEVTNGDGEKIAIFQGMVYRKSPRR"
     gene            complement(3978..4454)
                     /locus_tag="PKM87_04255"
     CDS             complement(3978..4454)
                     /locus_tag="PKM87_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNQ29313.1"
                     /translation="MDVLKIPKMEKQEYDRIIHEGHVSRIAFRGENFPYIAPFMYVFD
                     GKYLYFLSTKYGRKIGYFRSNPAVSVEIEQYSPDLSTFRFVSLQGRLEEVRDPVISRD
                     VRARFVDMMKRRGLSSAAMSALGHSPEDPPEKILEEERNLVWKLVGVKDIVALKNA"
     gene            complement(4537..5007)
                     /locus_tag="PKM87_04260"
     CDS             complement(4537..5007)
                     /locus_tag="PKM87_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29314.1"
                     /translation="MAFDCRQCGRCCMHLGDYIEIEREVGPFEFTCCSVSSGTTFTAR
                     VDADKQDLFSDRTWIREHPAACPFLRPSGTRIVCTIHHTSPAQCKAYRCVIARIYSPD
                     GVEMGYITGTRALHSSDPALRAAWDEVERAIAACPEDAESRIAPLLARRGYRCE"
     gene            5178..5603
                     /locus_tag="PKM87_04265"
     CDS             5178..5603
                     /locus_tag="PKM87_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNQ29315.1"
                     /translation="MTAEGGSDECLQCGLCCKIFGDRIQPTLENLFSWMEDGREDILS
                     FFMACLEDGRWVRCTEIAPGDLSGIHAVELRDPATGGYLPVCPFLKRVAKNRYLCGIH
                     AVKPEMCRNYQPWIWGETYFNRCKALKKREEYLWAHIFE"
     gene            5686..5961
                     /locus_tag="PKM87_04270"
     CDS             5686..5961
                     /locus_tag="PKM87_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNQ29316.1"
                     /translation="MKEILARVSGRVQGVGYRYFVRDQAERLGVTGWVRNNADGTVTI
                     SAAGDYGVLDEFLSLVRAEGDRVIHVDDIDVEWQDRCNSGNGFEIRK"
     gene            5998..7257
                     /locus_tag="PKM87_04275"
     CDS             5998..7257
                     /locus_tag="PKM87_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HNQ29317.1"
                     /translation="MQYELIIFLFALIFITVMSIWVRAPPFFTLLAGALFYGLLAGME
                     PDQVIIAAIGGLGKVFALFAVIILCGAVIAKTLQAQGQIEGLVADLEKRTSNTHALSG
                     LAGYLFAIPTACCITAFVMLTPVLERLSRVGEKRKYLYLAAVGSVLSYTLIYPTPVVI
                     PLFSGLAGGTSPLIFDLVAVPLSLLALGAAVFAFRARSRKGGNGGEDAGNTTAGGGIH
                     WRAWAPFLAILASIPVFWLAIPLSHESMIQCIMIAGLLTAMVLGTPEARNEGLNRGMK
                     HAGIILFDICGAGAFGSVIVASGFSEEVFPGISVALPLVLIPFSFTAIIQAAQGSRVV
                     SAIIASQVLAGTAVAASMHPVPLILSVAAGTCVISYLSDPYFWLLQRTTGGSVGEVVR
                     NYTFPLILCGLFVLAVAVLLQAALGAV"
     gene            complement(7286..8362)
                     /locus_tag="PKM87_04280"
     CDS             complement(7286..8362)
                     /locus_tag="PKM87_04280"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNQ29318.1"
                     /translation="MCNTIQETTPVTPLDPSDARCNAVKYAILAPSPHNTQPWRVAFQ
                     GDERIILSIDHSRLIPGCDPLGRQAFISVGAFLENLDLAAKNSGLRADIDLFPAGWPD
                     SRLVQDTPVARVDLVEDPHIEPDPLFLQIPLRHTSRRRFEKRKVPLDAAGEITAAYDF
                     SLVPLGFSHDDDLIHAIADLAADAMEIELADPERFRETLQYFRFTDAEAHTAQDGFGH
                     AQSGYGPVMRFFIGRFILPRSKAASSPESFSRMAKRSTREQAASAGGIGWLSTKGDHR
                     VDQVRTGRAFQRVHLKACALGLALHPMSQLLADYPGMAGLRSVLRDLLGVPDTQAVQM
                     LFRMGYARPVLPTPRRRPGDFFFP"
     gene            complement(8702..10102)
                     /locus_tag="PKM87_04285"
     CDS             complement(8702..10102)
                     /locus_tag="PKM87_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ29319.1"
                     /translation="MQGHVQGVNPRTRTLILVVVTIATFLNPFTGSSINLALPLIGEE
                     FGTDAVILSWIPTSYLLSTAIFLLPAGRFGDMMGRGRVFAWGTLLYTSASLLTVFTPH
                     IPFLLGCRFLQGLGSAMVFSNAVAIIAEVYPPGERGRALGLNVMAVYGGLTLGPFLGG
                     LLTQYLGWRSIFGVTIFLGAVVLASVRRLPASLSGTVREPFDIRGAVLSAAIMVLFFV
                     GISTLPATSGIAAVVLSLGLLLVFVRVEDAAPSPLFPLHLFRENRSFAYSNASALINY
                     CATFAVTFLMSLYLQVVQGLNPAFAGLVLLVQPMVQMIFSPFTGRLSDRVPPGDLASI
                     GLIISAASLLGFSFLSEETPLFVVILLLAVLGTGLAFFSAPNTNAIMGAVSRHHYGVA
                     SAMLATMRSIGMMLSMGMVMVAFALLLGDVALTPAVSGPFLTCMQAVFLVLFLLSVAG
                     ALVSRQKETGLQFPAG"
     gene            complement(10250..10675)
                     /locus_tag="PKM87_04290"
     CDS             complement(10250..10675)
                     /locus_tag="PKM87_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNQ29320.1"
                     /translation="MSIYTVCQKSVVSVTPDTSIRFVADLMRGKNLGCVVVAAEQKPV
                     GIVTDRDLALRRDRICGEEADEAHVSTIMTREVCTIRKDTGIFDAIQEMKSSGVRRLP
                     VVDAGGRLVGLITLDDIIRLLARELGEVARIIGKESPKI"
     gene            complement(10726..11496)
                     /locus_tag="PKM87_04295"
     CDS             complement(10726..11496)
                     /locus_tag="PKM87_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ29321.1"
                     /translation="MIPRPTWNAWKVWRRNWDVFFKTYRVNFLPPFIEPVLYLLAIGY
                     GLGMFVGEIEGIPYVLYIAPALLAISMMNSSFFECTYSSFVRMYYQKMFDAMVSTPLS
                     IEDVIAGELLWGATRSFIYATAMLVVLVAFGVVSLPTSLLVIPFSFLAGLLFAGIAMC
                     FTAITPGIDTLNYPAFLFITPMFLFSGTFFPLSVLPGPLQVFALAVLPLAQVVTVTRS
                     LTLASLSPLFLYSLAWIGAVTAVFFFLSIYLMKRRLVV"
     gene            complement(11503..12411)
                     /locus_tag="PKM87_04300"
     CDS             complement(11503..12411)
                     /locus_tag="PKM87_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ29322.1"
                     /translation="MQPIIEARGLRKDFGALKAVDGISFSVMPGEVFGFLGPNGAGKT
                     TTMRMIECVSPATAGMLTVFGIDVNRDAREIKRRLGVVPQETNLDPDFTCFENLVVYA
                     RYFGIPRAEARERAEELLSFVQLLEKRDVPIEKLSGGMKRRLILARALVNSPGLLVLD
                     EPTIGLDPQARHLMWEKLKALQAAGNTIVLTTHYLEEAERLCNRLVIMDHGKILIEGT
                     PAELVHQYAGEEVVEVDHTPEVVACLQEMGARYEVFGDMIEVFTDQPREVAKRLLSDC
                     NPGRVFVRRGSLEDVFLSLTGRVLRD"
     gene            complement(12728..12907)
                     /locus_tag="PKM87_04305"
     CDS             complement(12728..12907)
                     /locus_tag="PKM87_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29323.1"
                     /translation="MKPLMKGATTTTMRPGHMARSMSLATACAVKYESFSLPSLISLT
                     AAPPMPPSAPRAARL"
     gene            13049..14302
                     /locus_tag="PKM87_04310"
     CDS             13049..14302
                     /locus_tag="PKM87_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ29324.1"
                     /translation="MPIQELAVSNFKSFRHLEIQPDNFNVIIGSNASGKSNFIQVIKF
                     IRDIAKYGLSNAISLQGGVEYLRNTRIGAGEPFSINIRYLLEEQKTDIVAMSGGFPVS
                     FRPREICYAFSLAFPDAGETFEIASDALEVEGEFFRQEEGEQRSAGTGTIALVRAGGP
                     IQYCICPPKGLELREEELFPYYFQRVDIPEGTLLMTLPLFVPGIPSLEWIFRGMKVFD
                     FDPRLLKKAVPIMGKTDLEKDGSNLAIVIKRILEDPGKKETFTRLLRDVLPFVDDVAV
                     EKFMDMSLFLKLREVYSREKYLPASMISDGTINICALLIALYFEERPVTIIEEPERNI
                     HPYLISRLVEMLKDASRSKQVIVTTHNPEMVKHVDIHDILLISRDSEGFSRLIRPANK
                     KEIRAFLEDEIGIEELFIQNMLGIE"
     gene            14299..14886
                     /locus_tag="PKM87_04315"
     CDS             14299..14886
                     /locus_tag="PKM87_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29325.1"
                     /translation="MTERRLYILVEGNDDERFFGRIILPLISHRYRSVRLIKYACLKS
                     ARVCKFIQSIRRTGDEYLVVTDIDQAPGVMAKKQVILDRFCVVNPGAVMVIIQEIESW
                     YLAGLGPSDARILGVSVPDSTDHLTKEEFNRTIPSQYVSRIAYMLEILSRFSIPEACR
                     KNRSFQYFMGRHHIAPPARSLPETGELKDQGEPES"
     gene            14953..17007
                     /locus_tag="PKM87_04320"
     CDS             14953..17007
                     /locus_tag="PKM87_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ29326.1"
                     /translation="MWKHRIPAILEQKNFWTVILVISTFFAFVLNFYSLERDLTVVTP
                     HLFYIPIVIAAYWFPRRGVIYTVAIAMGYLAMAYIEGYPDIEILTQSTARFYVFVAIG
                     VIVASLSNNLKEQEDRYHGIFDYSEAGVFLAMNLRHGFVIEEVNERGANLLGYRAREL
                     VGRTLLDFFVEKQEKALIQEKVSPGGAVTEFECHLKRKDGYVIHGLMSAGPLPGRKVV
                     FTLVDITGMKKAEEELRESKERYQGLYTNAQVGLARASVTDGRILEANDQMANMFGYS
                     SVDEFIREFRFPEHYADKGVREQLFATLADNDTISNFEARFFRKDGSILWARFWARIF
                     PEKGYLEEVFTDITEEKLSHRALDESEERYRKLVESLPDYVLVFANDRILFVNPSAAN
                     TMGKTPDEMVSHPIYDYLSPESVETIRTHSKKRVAGGHVNPYEIDLSLNDGRSRTVLA
                     DATPITYRNQDAILAVLTDITDRKRADEKLKASKDQYMATIDAMVDGIFLVNQDLEIV
                     LVNSSFRRWLERIGRHHEVVGMNAGDALPCVGDPGLKAIKNVFSTGKILETTVDFHLN
                     GRGYFFENRHIPVFEKKHVARVMTIMRDITRSRMIEEEKRLAYEQIEKNIEQFAILGD
                     HLRNPMQVIVGLADLEGGPLAEQIHRQTEEIDRTVSQLDRGWIESEKIREFIRKYYGI
                     GK"
     gene            17217..17708
                     /locus_tag="PKM87_04325"
     CDS             17217..17708
                     /locus_tag="PKM87_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HNQ29327.1"
                     /translation="MKLEIKDAPGQLVAALKPISEVGGNIIAVIHQRDEKQRGGFLSV
                     QIVLELPENRLKDLTRLIREQGVNIQRIGHERLLYQKAVMVIGHLIHTDLGDTVDRID
                     RTGFAEVTELNISMPAISQVSSARLLIKSVNKEDMEQAMAILRRVAKEKEFLLVEPLE
                     EVE"
     gene            17705..18703
                     /locus_tag="PKM87_04330"
     CDS             17705..18703
                     /locus_tag="PKM87_04330"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNQ29328.1"
                     /translation="MRVALLGMGSVGRGVLQMIGEKDLGITVTAVADSRSALIDRDGI
                     SIEEVLAAKARNGFCGDKAISAMDVVTGSAYDVLVEVTPTNATTGEPALTHMLCALER
                     GRHVVTSNKGPISREFSRLREIAVEKGIQLRYEATVGGAIPIMHTLEQGLAGNRVLAI
                     FGVLNGTCNFILTRMAAEGLTYEQALLEAREMGYAEADPTYDVKGIDPAIKLVILANT
                     FCSHSVSLEDVDITGIDRLTNDALRLAEEQQCTIRLIAEAIPDRGILRVAPRLLPLSH
                     PLVVEGTLNAITIETDMAGEITLIGKGAGSVETASAIIGDLLYIRDTHAGRHSKTA"
     gene            18675..19790
                     /locus_tag="PKM87_04335"
     CDS             18675..19790
                     /locus_tag="PKM87_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ29329.1"
                     /translation="MRGVIQKRREYLHLMRACTLERGHFTVTDIQKGAGVPRSTAQDW
                     INRLLDEGCVVIRERKMGRNPAKYAAISALPSSACRRIFTTIDGDSVEIHHECMSSAC
                     AAFCAYHHSHARGILQGVHRDGTLIREWARLGREDIDIGLFPSSAVGVAGVERRGEDI
                     IQHIRCIGGPAYSLTDMMSHAEGVCEVSIQRSGEVVEGSVRTRALAHLIIGIDDTDSR
                     EGGATFALALALLQHLETLKGVLPISHHVVMLNPAVSEKTAGNSCSYIEVAIPPGMYS
                     RVRDRSLVFVEDEALSREWGMAFKQGFQVPQSLRAYGDMARRGIVTREEAAETAAQHH
                     IEVIGGRGIVGALAAVALSGLSHEVLLRAEARIPGTS"
     gene            complement(19787..20782)
                     /locus_tag="PKM87_04340"
     CDS             complement(19787..20782)
                     /locus_tag="PKM87_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HNQ29330.1"
                     /translation="MKKILITVGNEDAANIPQLLKGHFHLRENFGEKTRFSIVVPDDE
                     LDETVAKIKVTLGYMDKNTLVEVYSPDFTIYPLFDEKKEKEETPDAKKKKRNHSPIEK
                     LLDTVKSYERLDADILSLAAVASIVGLVGLFLNNVGIIIGAMLIAPMIGPIYAFALHS
                     ATGNAMGVLRDILNIIFLILSFILFSLVVTWILGLFIPLEVTGEMLSRTHSSPIYAIM
                     AIFLGFATIVALSKGIPDSIAGVAVAAALLPPALVSGLTVVLYPEGTLGALVLTLQNA
                     IGLMAGSIMATIALQIQPRSYREKAAAKAILRRVAILLLILVLLLALIPLITVSG"
     gene            21008..21901
                     /locus_tag="PKM87_04345"
     CDS             21008..21901
                     /locus_tag="PKM87_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNQ29331.1"
                     /translation="MPVEKTGKEIPAQLKAIIYRFQRDEVTEYQIYRMLAKAVRDPHN
                     ASVLLRMADQELGHSRIWEHYTGKVAGPAVARVWFYYIIARLLGYTFAAKLMENRESS
                     AIQDYGTLVGKIPEVEGIIAEEEQHEMEILGMLEEDRLRYVGSIVLGLNDALVEFTGS
                     LAGFTFALQDTTVIAAVGCIMGVAAALSMASSEYLSQKADQTGREPAKAALYTGGAYI
                     FTVFLLVVPFLLMGNPFSALVVSLITAVAIIVCFTFYTSVAGGLPFWRRFLEMVAISL
                     GIAGVSFLIGLLIRGLFNVDV"
     gene            complement(21885..22607)
                     /locus_tag="PKM87_04350"
     CDS             complement(21885..22607)
                     /locus_tag="PKM87_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29332.1"
                     /translation="MKHQYLGDTRDLFKYDLIETLLSTLPSLTQAFFIPMLTPDDGSG
                     HGRRTGYFRTRAGNLNAGLADFLSKLRMRKSRDCFMVRDYFQEKSCEVSFYRARFTHR
                     GRGAYFSRIAKKVHGLPHTLVFLDPDTGLEVASPGAGHLLYSEVSLIARSLGPGSLLM
                     IYQHIPRTPRIAYIEHRCASLFEHTGILPSWISDGQVVFFFLCPEQNVGYELDHCLER
                     YRIRYPLLLARQEEGAGFIHRR"
     gene            complement(22734..23006)
                     /locus_tag="PKM87_04355"
     CDS             complement(22734..23006)
                     /locus_tag="PKM87_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNQ29333.1"
                     /translation="MTLASTPAQVNGPDIRGPVNSIDCLPSSAQAVFKLLGDGKERTL
                     NEIIGRVAFSPRTIRNALNKLRQSGLIVAKFNFKDARKPLYKLKTG"
     gene            23206..23994
                     /locus_tag="PKM87_04360"
     CDS             23206..23994
                     /locus_tag="PKM87_04360"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNQ29334.1"
                     /translation="MRTDAMSTPLRNQDSPGRYIQIVASDQGLTSIDSQLKGKILEML
                     QEKDMDFEEIVARSGRAKSTISAHMKALSLAGITTTRPDPRDARKRIFSLNGRVLVRA
                     HTDDTELLWADRFFPDALPGDATTRDVFRFILTSLRVSLLAEGISIYPILERAGRKAG
                     AAIYPQVRAAELSVFLGNLSAFWARFGLGEMEVEREDPLTIVVRDCFECMDLPITGRP
                     ECAFDTGLLATLFSAYYGEMHEVVETHCYATGSNFCRFEVWNAR"
     gene            complement(24010..24513)
                     /gene="sixA"
                     /locus_tag="PKM87_04365"
     CDS             complement(24010..24513)
                     /gene="sixA"
                     /locus_tag="PKM87_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HNQ29335.1"
                     /translation="MDLYIMRHGKAGVADHSDPRDSERCLSKPGREEISLAGAWMKAR
                     EIEFSTIASSPLRRAAETASIIARALGDPDRVTFWDELAIGPPPGTVVQKIREIMPEN
                     RILLVGHEPQLSSLVSLLICSRHECNIVLKKGGIARVRFHGDPGSGELLWLLSPGMIQ
                     EWHSKDR"
     gene            complement(24854..24936)
                     /locus_tag="PKM87_04370"
     tRNA            complement(24854..24936)
                     /locus_tag="PKM87_04370"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24900..24902),aa:Leu,seq:tag)
BASE COUNT         5368 a         6915 c         7526 g         5133 t
ORIGIN      
        1 tattcctgtt caccccggac ttctccgtcc tctcagatcc cctcctgtgg agcgccgcgt
       61 ttgggcaggc gttcttctcg ctctcggtgg ggaccggaat cctccttacc tacggtgcct
      121 acatggagcg ggggatgcac atcccccgct ccgccctcat catcaccttg tccgacattg
      181 cggtcgccat cctcgcagga atcgtcatct tcccgctggt cttttcgttc ggtctgacac
      241 ctgcagcggg agccgaactc gctttcacca ccctccctat ggcattcgcc gcgatgcccg
      301 gaggagtgtt ctttgccgtg tcattcttcc tcgtcctttt ttttgccgcg ctcacttcgt
      361 cggtatcgat gctcgaggtg agtgtctcat ccctgcagga ggcagccgga tggtcgagga
      421 agaaggccac gctcctcctc ggaatcctca tggcagccgt gggccttccc tcggcgctga
      481 gctacagctc ttttcacctg accttcaacg gtataaaggt acttgacttc atggacgaga
      541 cagtggggac actggggctc ccgattgccg ggattctcct ggcagtcgcc ttcacctggt
      601 ttgtcccacg ggacgtgctc tcaagggaaa tcgggacagc gctgtccagg gtcgtgcatc
      661 ctctctgccg gtttgtcatc ccctcggtcc tggtcgtgac caccgtcgcc cgcctgcttg
      721 cggggatcga tctcgccgat ctccgcctcc tccccggaag caggtggatc ggtactgtcc
      781 tccaggcaga agggatggcc gccatcattg tgatcgtcct cctgctgatc atgctgctct
      841 gccgcgttgt gagctgcccg ctcggtgcct ggctgctcgg aaagagatga attatttttc
      901 actcgcagca ggggagttca accatgcaac tccctgaagc ggcggcggaa cgccaggtag
      961 aggatgattc cggcgatgat gactgccagg gcgaccccga gcccgaggac caggttctcc
     1021 acgatcaacc gccagatcag ctcggagccg agggcgaaga tcaggcactc ggccgatgca
     1081 cctattccaa tggcaaggag gatcttccca ggagagagcc ctgccatcat cccgacaagc
     1141 gtcgctccga caccccccgt tccctggagg gggagaagca cgaagcccgc gacgcccagt
     1201 accgaccatc gggagagcca ggggtgtttt tccatgaagg cgtcccctga tgcgagaaac
     1261 cgggagatcc agggcccaag tacgggaatg cgaagtgcaa tccggaagtt gaggatgaca
     1321 aagaggctcg tgatgacatc gaggaatgcg agtgaggccg ccatcagcca ccagggtatg
     1381 ccaagcccga ttccaagcgg gatgatggac tcctttcctg cgggggggat atagtagact
     1441 atcatcagcc ctccgaggat cagggacttc tcaggaggga ggacaagaag gctgctgaag
     1501 aaataggtga aagcgaggag aacggggaat aaaagccgca acacggtaaa ggtgagcgaa
     1561 tcatccttcc cggaagtaaa tagagagatc ccacccgtga acggcattcc tgttagagta
     1621 aggtagggac aggcacttta ttccttccct cctctcccgg acgggcgtcc cagtacacgg
     1681 aaaggggcgg tcaagttgtc gaccggaccc gtgtggtgag aaaaacccgt cttgtgggtg
     1741 atggcaggaa taatgaaaga aacactgcac ggattccggg aatggccccg gaacatcgtt
     1801 gaatgaaaaa aaccagggtg agaacatcaa ggttaccctt atgtgacagg gatgtcaaat
     1861 acctgttaca cttgagcggc atgaaacaga acacgttttt tctcctggca gggattgtag
     1921 gccttgtaga ggtagggctc ttttggctct cggtcgtgat gcgaaatccc ctcatcatca
     1981 cggcagtgtt catcatgggt gtcgggcttt tgtacgtggc caggatgact atcacagacc
     2041 aaagggagga tgaacgggcg gtactcatca cccagaaggc agggtcaagg acgctcgagg
     2101 tcttctgggt cctcttcttt gcagtcagcc tcgggagtgc ggtcattggg ttcagcaccc
     2161 cactcggtct cccgcgcccg agggaattgc cgcacgactt tccaagggac gagccgcacc
     2221 tcgggtactt cggcatcctc cagatgatcc tgctctgctg catcgcgttc ctctacattg
     2281 gattccgcgt gtattacgct cgaaaatacg gggaatggga gacagatgaa gaataagatc
     2341 aaggtattcc gtgccatgca cgacctgacc caggaggcgc ttgcgcagaa gctcaacgtg
     2401 acccgccaga ccatccttgc catcgagaag gggaagtatg acccatccct cgagctggct
     2461 ttcaagatcg cgaggttctt tggcgtctct gtcgaagagg tcttcattta cgactgaacc
     2521 ccatgcgatc gatcacgaat gcaggtttga tcgccctgtt aggcgcgctc caacatggcg
     2581 gcgtctcccg gcagcctcca atgccggcgg cgtccatacc gggacgtatc cgagaaaaag
     2641 gcgcaaatcg tctttcttct ctccaattct ccacggagag cacaaggttc accatctccc
     2701 cgagccccct cttctgtaga gaatgactgc gacaagtctt ttcatcctgc agcacgaaaa
     2761 agcggaacct gcgggtattt ttgaagaggt cgcccgcacc agaggaatat ccagcaccgt
     2821 tgtgcccctg ttcgaaacac acgaagttcc gcctgtcacg tcgacccatc tcctcgtcct
     2881 gggtggatcg atgagcgtga acgatggtgc ccgatatccc ttcatcgacc aggaaaagag
     2941 gctcatccga tcctggattg caaaggggcg accggtcctc gggatctgcc tcggcgccca
     3001 gatgatcgcg gctgcctgcg ggggagagat ctcaccgtgc aggaaagaga ttggctgggt
     3061 tccactggag gtcattgata tcgctgtgct ccccggggtt cctcgctcgt ttatggcgtt
     3121 ccagctgcat ggtgagacgt tctcgctccc tgccggggcg gacctggtct gccggggcaa
     3181 agaggttccc caccaggccc tcgcagtcgg gagtgcgctc gggctgcagt ttcacctgga
     3241 gccgacggag cagatgatcc ggctgtggct ctctgaattc catcccgggg aacagtcccg
     3301 gatattgcag gacactgccc gttacctggc cgagtcacac aggctctgcc gaatcgtctg
     3361 cgatcggttt ttccaggccg ctccccgggg gttctgctgg cgctgcccgt gagtgctgca
     3421 tttcagggcg accgcgcgct cccccggatt ctttccaccc gctacctgcg gggtgatttc
     3481 cgatacacca tgccctggaa gattgcaatc ttctcgccat ctccgttggt cacctcgacg
     3541 gtgtaggtcg caagcttggg gttgagcgca aactcccttg cctcggcata gagcgacccg
     3601 ccccttgcag atttcatgta ggagatgctt gcattgatgg ctgcggccgg aatcccgtgg
     3661 gagttcgagg caagcgcaaa tgcggcgtct gcgagggtat agatagcgcc gccatgcacc
     3721 gtgccatggc tgttttcatg catttcccgg atctccatcc tgacccgggc cgtccctggt
     3781 ccgatttcga gcagttcgat ccccgtgtgc cgtgcgtagg cgtccttccc aaaaaagacc
     3841 tcggcgtctt ccatggtcgt agagtatccc ggtacctgga ttaaaacatg cgcacggtat
     3901 tcgaaagacc aaagaaaatg cctgttggtg gagggagtcg tcggtgacga aaaagtgaga
     3961 taaaaaaggt ggatctctca cgcgtttttg agcgcgacga tgtcttttac gcccacgagt
     4021 ttccagacga ggtttcgctc ctcctcaagt atcttctcgg gcgggtcctc gggagagtgg
     4081 ccgagcgcgg acattgcggc cgatgaaaga ccccggcgct tcatcatgtc cacgaacctg
     4141 gcgcggacat cacgggaaat cacagggtcc ctcacctcct cgaggcggcc ctggaggctc
     4201 acgaaccgga acgtggagag atctggcgaa tattgctcga tctcgacgga gactgcggga
     4261 ttgctcctga agtatccgat cttccgcccg tacttcgtgg agaggaaata gaggtatttt
     4321 ccatcgaata cgtacatgaa cggagcgata tagggaaaat tctccccgcg gaaggcgatt
     4381 cggctcacgt ggccttcgtg aatgatccgg tcatactcct gcttctccat ctttggaatc
     4441 ttcaggacat ccatgaaaaa ggagtgaccg ggcgccatat taatgtattc tcaggggaag
     4501 gggggggcgt tccgggcaga acaggcctgg cccccttcac tcgcaccggt atcctctccg
     4561 ggccagcagt ggggcgatcc ggctctcggc gtcctccggg catgcagcga tcgcccgttc
     4621 cacttcgtcc caggccgccc tgagcgcagg gtcgctgctg tggagggcac gcgtcccggt
     4681 gatgtatccc atctcgactc cgtcagggga ataaatgcgg gcaatcacgc agcggtatgc
     4741 cttgcattgc gcggggctcg tgtggtggat agtgcagacg atgcgggtac ccgaagggcg
     4801 gagaaagggg catgcagcag ggtgttctct gatccaggtg cggtctgaaa agaggtcctg
     4861 cttgtcggca tcgaccctgg cagtaaacgt ggtgccggac gagaccgagc agcaggtgaa
     4921 ctcgaaggga ccgacttccc gttcgatctc gatatagtcc ccaaggtgca tgcagcatct
     4981 gccgcactgc ctgcagtcga atgccattgc cataccattc ggaccgaata cgggataaat
     5041 ccaggggcac gatctgccgg aatggcagtg cacggatggc tcgaccgcgg tggagaggaa
     5101 tgaaagagag ataagggttc gggagaacaa taccaccaat gggatttgtg aatcctgaac
     5161 agtctgggga caaccgcatg actgccgaag gggggagcga cgagtgcctc cagtgcgggc
     5221 tttgctgcaa gatattcggg gacaggatcc agcccaccct cgagaacctt ttctcgtgga
     5281 tggaggatgg ccgcgaggat atcctttcgt tcttcatggc atgcctcgaa gacgggaggt
     5341 gggtgcggtg cacggagatt gcccccggag acctctcggg tatacacgcg gtggagctgc
     5401 gggaccctgc cacgggcggc tacctcccgg tctgcccgtt cctgaagagg gtcgcaaaaa
     5461 acaggtacct atgcgggatt cacgccgtga agcccgagat gtgcaggaac taccagccat
     5521 ggatctgggg cgagacctac ttcaaccggt gcaaggcgct gaaaaagagg gaggagtatt
     5581 tgtgggcgca cattttcgaa taaggaggga agccagttgc ctgccggtat ctgcccaagg
     5641 atatgggagg ccaaagacac tggtatcata ggaggggacg aacgcatgaa ggagatcctg
     5701 gcacgggtat cagggcgggt ccagggggtc gggtaccgct acttcgtgag ggaccaggca
     5761 gagcgccttg gtgtgacggg ctgggtgagg aacaacgccg atggcaccgt caccatttct
     5821 gcggctgggg attatggggt ccttgatgag ttcctttccc tggtccgggc tgagggggac
     5881 cgcgtcatcc acgtggatga tatcgacgtg gagtggcagg ataggtgcaa ttctggtaac
     5941 ggctttgaga tacggaagta gggcccggga agtacgggcg agaatgcgga agtaaccatg
     6001 cagtatgaac tgatcatttt tctctttgcc cttattttca tcaccgtgat gagtatatgg
     6061 gtccgggccc ctcccttctt caccctcctg gcgggagccc tattctacgg gttactggct
     6121 ggaatggagc cggatcaggt gataatcgca gccatcggcg gtctcgggaa agtatttgcc
     6181 ctctttgccg tcattatact ctgcggggca gtcattgcaa agacactcca ggcgcaggga
     6241 cagattgaag gcctggtggc agacctggaa aagaggacca gcaacactca tgccctctcg
     6301 ggcctggccg ggtacctctt tgccattcca accgcttgct gcatcaccgc gtttgtcatg
     6361 cttaccccag tgctcgagcg gctgagccgt gtgggtgaga agaggaagta tctgtacctt
     6421 gctgcggtgg gaagcgtcct ctcctacacc ctcatctacc cgacgccagt ggtcatcccc
     6481 ctgttcagcg ggctcgcggg aggaacctcc ccgcttattt ttgaccttgt cgcagtcccg
     6541 ctctcgctgc ttgccctcgg cgcagcagtc tttgcgttcc gggccaggtc gcggaagggc
     6601 gggaacgggg gagaggatgc ggggaacaca acagccggcg gagggatcca ctggagggca
     6661 tgggccccgt tcctggcaat ccttgcgtct atcccggtct tctggctcgc cattcctctc
     6721 tcccacgaga gcatgatcca gtgcatcatg atcgcggggc ttctgactgc catggtatta
     6781 ggcacgccgg aggcgagaaa cgaagggctc aatcggggaa tgaaacatgc agggatcatc
     6841 ttgtttgata tctgcggggc aggggccttc gggagcgtaa tcgttgccag tggattttcg
     6901 gaagaagtct ttcccgggat ctcggttgcc ctgccgcttg tcctgattcc gttctcgttc
     6961 accgcgatca tccaggctgc ccagggctcg cgggtggtaa gcgccataat cgcgtcgcag
     7021 gtcctggccg ggaccgccgt tgcggcatcg atgcacccgg ttcccctcat cctctcggtt
     7081 gcggccggaa cctgcgtcat ctcctacctc tctgacccct atttctggct gctgcagcgg
     7141 accaccgggg gaagcgtcgg tgaggtggtc cggaactaca cgttccccct catcctctgc
     7201 ggcctttttg tcctcgccgt ggcagtcctg ctccaggctg cgctcggagc ggtgtaacca
     7261 cccctgccgg gaacgagtgc ccgcgtcagg ggaagaagaa atcccccggc ctcctccggg
     7321 gcgtaggcag gacaggacgt gcatatccca tgcgaaagag catctggacc gcctgggtat
     7381 cagggacgcc cagcaggtct ctgagaaccg accggaggcc tgccattccc gggtaatcgg
     7441 caagaagctg gctcatcggg tgaagcgcga ggccgagagc gcatgccttg aggtgaaccc
     7501 tctggaaggc tctcccggta cgcacctggt cgaccctgtg gtcccccttt gtcgagagcc
     7561 aaccgatccc gcccgcactt gccgcctgtt ccctggtgct ccgcttcgcc atgcgcgaga
     7621 acgactccgg ggaagaggca gctttgctcc tggggaggat gaacctccct atgaaaaacc
     7681 gcattaccgg tccatagccg gactgggcgt gcccgaaccc gtcttgcgcg gtgtgcgctt
     7741 ccgcgtccgt gaacctgaaa tactggaggg tctcgcggaa ccgttccggg tctgcaagtt
     7801 caatctccat cgcgtctgcg gcaaggtccg caatggcgtg gatgaggtcg tcgtcatggg
     7861 agaagccgag aggcacaagg gaaaagtcgt aggcagcggt gatctcccct gcggcatcaa
     7921 gggggacctt ccgtttctcg aagcggcgcc ggctggtatg ccggaggggg atctgcagga
     7981 agagcgggtc gggctcgatg tgcggatcct ctacgaggtc cacgcgggcg acgggtgtgt
     8041 cctgcaccaa acggctgtcc ggccaccctg ccgggaagag gtcgatatct gcacggagac
     8101 cggaattttt cgcggcaagg tccaggttct cgagaaatgc ccccacggag atgaaagcct
     8161 gccttccaag agggtcgcac cccgggatca gccggctgtg gtcgattgag aggatgatcc
     8221 tctcatcgcc ctggaacgcg actctccagg gctgggtgtt gtgcgggctc ggcgcaagaa
     8281 tggcatattt gaccgcgttg cacctggcat ctgagggatc gaggggggtc actggagtgg
     8341 tctcctggat tgtgttgcac attctctcat cctgcaggga taagagctat gcgcatcaag
     8401 gcgggccggc acctcgttcc ggcgtctccc atggagtgat ggggtcggcc gatggcgacg
     8461 acgagcgttt ccattcgtca aggtattcgc tgcggtgacg acgtggtccc ttatgtgcaa
     8521 ccgccataga gcgcccgtag gcccactcat caggggaaag ggataaatgt gagtgggcat
     8581 cgtgtccccg gcacgctatg tacaacacac cattgggatc agcggcatgt agctgaatcc
     8641 aggggggcgc cgcgctcacg actcccagca gcatgcacct ttcccgtctc cttctgccgg
     8701 ttcagcccgc agggaactgc aggcccgtct ccttctgccg ggaaacaagt gctcctgcga
     8761 ctgagaggag gaagagaacg aggaagacag cctgcatgca ggtgaggaag ggcccggaca
     8821 ctgccggcgt gagcgcgacg tctccgagga ggagggcgaa cgcgaccatc accatcccca
     8881 tcgagagcat catcccgatg gagcgcatgg tcgcaagcat cgcggatgcg acaccgtagt
     8941 ggtgccggga gaccgcgccc atgatggcgt tggtgttcgg tgcggagaag aaagcaagac
     9001 cggtcccgag gacggcaagg aggagtatta ccacaaagag cggtgtttct tccgagagaa
     9061 acgagaaccc gaggagactt gccgcggaga tgatgaggcc gatggatgcc aggtcccctg
     9121 gagggaccct gtcggagagc cttcctgtga acggggagaa gatcatctgg accattggct
     9181 gcacaaggag gacgaggcct gcgaatgccg ggttgaggcc ctgcaccacc tggagataca
     9241 ggctcatcag gaaggtgacc gcaaacgttg cgcagtagtt gataagggcc gatgcattcg
     9301 aataggcaaa cgacctgttc tctcggaaca ggtggagggg gaaaaggggg gagggtgccg
     9361 cgtcttccac ccgcacgaag acaaggagga ggccgagtga gagaacgacc gccgcgatgc
     9421 cggatgttgc cgggagtgtc gaaatcccca cgaagaagag aaccatgatc gcggccgaga
     9481 ggacggctcc gcgtatatcg aagggctctc ggacagtccc ggagagggag gcgggaagcc
     9541 tgcggacgga cgcgagcacc accgcgccga ggaagatggt gacgccaaag atgctcctcc
     9601 accccaggta ctgggtgagg aggccaccga ggaacgggcc gagggtgagg ccgccataca
     9661 cggccatcac gttgaggccg agtgccctcc cccgctcgcc gggcgggtac acttcggcga
     9721 tgatggccac ggcgttcgaa aagaccatcg cgctcccgag gccctgcagg aaacggcatc
     9781 caaggaggaa agggatgtgt ggtgtgaaga ccgtcaggag cgaggcagag gtgtaaagga
     9841 gcgttcccca tgcaaagacg cggccccttc ccatcatgtc gccaaagcgg ccggcaggga
     9901 ggaggaagat cgcggtggag aggaggtagg aggtcgggat ccaggagagg attaccgcat
     9961 cggtcccgaa ttcctcgccg atcaggggga gcgcgaggtt gatgctcgag cccgtgaacg
    10021 ggttgaggaa ggtggcgatg gtcaccacca cgaggataag ggttctggtc cggggattta
    10081 ccccctgcac gtggccttgc atggtacaat caccgggaac gcaacggact aaatgcggcc
    10141 cctgtggtgg atatcgtaaa taatgaggag attggacgtc atcaaagttt gcggatacgg
    10201 cacgctggag gggggggagt cgaagaaaac aggagtgggg ataaaaaggt cagatcttcg
    10261 ggctctcttt cccgattatc ctcgcgacct ctcccagttc ccgcgcgagc aggcggatga
    10321 tgtcatcgag ggtgatcagg ccgaccaggc ggccccccgc atccacgaca ggcagccggc
    10381 ggacacccga cgacttcatc tcctggatgg cgtcgaaaat gcctgtgtcc ttcctgatag
    10441 tgcagacctc gcgggtcatg atggtggaga cgtgtgcctc gtccgcctcc tcgccgcaga
    10501 tgcggtctct tcttaaggcc aggtccctgt cagtgacgat gccgacgggc ttctgctctg
    10561 ctgcaacgac cacgcacccg aggttcttgc ccctcatcag atcggccacg aaccggatcg
    10621 aagtgtcagg cgtgacgctt accacgctct tctggcagac ggtatatatg ctcattcgag
    10681 ttcctgtctc ctactgtgtg catcgatagg ataaaaatgg ccctttcaga ccacgagcct
    10741 tctcttcatc aggtagattg agaggaagaa gaacacggcg gtcaccgcgc ctatccacgc
    10801 aaggctgtac agaaagaggg gggagagcga tgcgagggtg agcgagcggg tgacagtgac
    10861 cacctgggca agggggagga cagcgagggc gaacacctgg agcggcccgg ggaggaccga
    10921 gagggggaag aaagtccccg agaagaggaa catgggggta atgaagagaa acgccgggta
    10981 attcagggta tcgatgccgg gagtgattgc ggtaaaacac atggcgatgc ctgcaaagag
    11041 gaggccggcg aggaacgaga aggggatgac caggagtgaa gtgggcagtg ataccacccc
    11101 gaatgcgacg aggaccacca gcatcgctgt tgcgtaaatg aagctccgtg tggcgcccca
    11161 gaggagctcg cctgcaatga catcttcgat agagagggga gtcgagacca ttgcatcgaa
    11221 catcttctgg tagtacatcc gcacaaacga tgagtaggta cattcaaaga acgaggagtt
    11281 catcatcgag atggcgagga gtgcgggcgc gatatagagg acgtatggga tcccctcgat
    11341 ctctccgacg aacattccga ggccgtaccc gatcgcaagg agatagagca ccggctcgat
    11401 gaaggggggg aggaagttca cccggtaggt cttgaagaag acatcccagt tcctccgcca
    11461 tactttccag gcgttccatg taggtcgggg gatcatcaag catcagtccc gaagaaccct
    11521 ccccgtaagg gaaaggaaga cgtcttcgag cgatccgcgg cgcacgaaca ctcttcccgg
    11581 gttgcagtcc gaaaggagcc tctttgcgac ctccctcggt tggtccgtaa agacctcgat
    11641 catgtccccg aacacctcgt accgggcgcc catctcctgc aggcaggcca cgacctcggg
    11701 ggtgtggtcc acctccacga cctcctcacc tgcgtattgg tgcacgagtt cggcgggggt
    11761 tccctcaatg aggatctttc catggtccat gatgaccagg cggttgcaga gccgctcggc
    11821 ctcctcaagg tagtgagtgg tgagcacgat ggtgtttcct gcagcctgga gagcctttaa
    11881 cttctcccac atcaggtgcc gggcctgggg gtcgagaccg atggtcggct cgtcgaggac
    11941 caggagcccc gggctgttca cgagcgccct tgcgaggatg agcctccgct tcatcccccc
    12001 agagagcttc tcgatgggca cgtccctctt ctccaggagc tggacgaagg agaggagttc
    12061 ttctgccctc tctcttgcct ctgcccgggg gatcccgaag tagcgggcgt atacgacaag
    12121 gttctcgaaa caggtgaaat cagggtctag gttcgtctcc tgggggacga ctccaagcct
    12181 ccgcttgatc tcccttgcgt ccctgttcac atcgatgcca aagaccgtga gcattccggc
    12241 cgttgccgga gatacgcatt cgatcatccg catggtggtg gtcttgccgg cgccgtttgg
    12301 cccgagaaat ccgaagacct cgccgggcat caccgagaag ctgatcccgt ccacggcttt
    12361 caatgcccca aagtctttcc tgagcccccg cgcctcgatt atcggctgca cagacagatc
    12421 acacctgcat ggaaaggatt tgtgcacccg gccacaacac tctttcggtg aagacagggg
    12481 attctcatcc ttctctcccg gccaggagag gtcatctaaa agtggtccat cagcgttcaa
    12541 taagaggatt gatggactcc cgacacatcg tacgccagat gcttgggatc gaatccgtcc
    12601 gccgtcaggc tatcctctcc gttggggcca atgtggggat tactgccatt gggtacctgg
    12661 caacggttta tatcgcccat accgcggatg ccgaggtgct gagtgcctac ttcctcttcc
    12721 tcgcccatta cagcctggcg gccctcgggg ctgacggcgg catggggggc gcagcggtca
    12781 agctgatcag tgaagggagg gaaaaggatt catacttcac cgcacaggcc gtcgccagac
    12841 tcatgctcct tgccatgtgc cccggtctca tagtcgtcgt ggttgcaccc ttcatgaggg
    12901 gcttcactgg tgctgggctc cttccctggc ttctcatggc gcttgccacc ttggcctccc
    12961 gtggccagcg cggatgtagg agtgcccgca atccccggtc atctcccttg gataaatacc
    13021 atatggtgga ggagtctaac cctatctcat gcccatccag gagcttgcag tctccaactt
    13081 caagagtttc cggcaccttg aaatccagcc ggacaacttc aatgtcatca tcggttccaa
    13141 tgcatcgggg aaatcaaact tcatacaggt catcaagttc atccgcgaca tcgccaagta
    13201 cgggctctcc aacgcaatct ctctccaggg aggagtcgaa tacctgcgaa atacccgcat
    13261 tggtgccgga gaaccattct ccatcaatat caggtacttg ctagaggagc agaagacaga
    13321 tatcgtggcg atgagcgggg ggttcccggt ctccttcagg cccagggaaa tatgctatgc
    13381 attctctctt gcgtttcccg acgcgggaga gacgttcgag atcgcatccg atgccctgga
    13441 ggtcgaagga gagttcttcc gccaggaaga gggggagcaa aggagtgcag gaaccggaac
    13501 tatcgcgctt gtccgtgccg gtggccctat ccagtattgc atctgcccgc caaagggcct
    13561 cgagctccgg gaagaagagc tctttcccta ctactttcag agggtggata ttccggaggg
    13621 aacactgctg atgacgctcc ccctctttgt tcccggcata ccctcgctcg aatggatatt
    13681 cagagggatg aaggtgtttg actttgatcc ccgccttctc aagaaggcgg tgcccatcat
    13741 gggcaagacc gatctcgaga aggacgggag caaccttgcc atcgtgatca agaggattct
    13801 cgaggacccg ggaaagaagg agaccttcac aaggcttctt cgggacgtgc tccccttcgt
    13861 cgacgacgtg gcggtcgaga agttcatgga catgtccctc tttttgaaat tgcgggaggt
    13921 ctacagcagg gagaagtatc tcccggcgtc catgatctcg gacgggacca tcaatatctg
    13981 cgccctcctt atcgccctct actttgagga gcggccggtc accatcatcg aagagccgga
    14041 acgtaacatc cacccgtatc tcatatcccg gctggtggag atgcttaaag acgcctcgcg
    14101 gtcaaagcag gtcatcgtca ccacccacaa cccggagatg gtcaagcacg tggatatcca
    14161 cgatatcctc ctcatcagcc gggacagcga gggcttctcc cggcttatca ggcctgccaa
    14221 caagaaggag atccgggcgt tcctggagga tgagattggc atagaggagc tcttcatcca
    14281 gaacatgtta gggattgaat gaccgaacgc cgtctgtaca tcctcgttga ggggaatgat
    14341 gacgagaggt tctttggcag gattatcctc cctctcatct cacaccggta ccgctcggtc
    14401 agactcatca agtatgcctg cctgaagagc gcaagggtat gcaagttcat ccagagtatc
    14461 cgcaggacgg gagacgagta cctggtggtc acagatatcg atcaggcacc aggcgtaatg
    14521 gccaagaagc aggtcatcct tgacaggttc tgcgtcgtaa acccaggcgc ggtaatggtc
    14581 atcatccagg agatcgagag ctggtacctt gccgggctcg ggccttcgga cgcacgcata
    14641 ctcggtgtaa gcgtgcccga cagcaccgac cacctgacaa aagaggagtt taaccggacc
    14701 atcccttccc agtatgtctc gaggattgca tacatgctgg agatcctttc caggttctct
    14761 attcccgagg catgccggaa gaacaggtcc ttccaatact ttatgggtcg ccatcacatt
    14821 gcgccgcctg cccgttcact ccccgaaacc ggggaactga aggatcaggg agagccggaa
    14881 tcctgatggc accttttatc atggtgagca cccaccgaat gtgtatctaa aaggggtgaa
    14941 agagaggtgg gggtgtggaa acataggata ccggcaatcc tcgagcagaa aaatttctgg
    15001 acagtaatcc ttgtcatcag cacattcttt gcattcgtcc tgaattttta cagcctcgag
    15061 agagacctca ccgtcgtcac tccgcacctc ttctacatcc caatcgttat cgcggcttac
    15121 tggttcccga gacggggagt catttatacc gtggcaattg ccatgggcta cctggcgatg
    15181 gcgtacatcg agggataccc cgacattgag atcctcaccc agtcaaccgc acggttctac
    15241 gtcttcgtcg ccattggggt cattgtcgcc tcgctctcga acaatctcaa agagcaggag
    15301 gaccgctatc acgggatctt cgattattcg gaggcggggg tcttcctggc gatgaacctc
    15361 cgtcacgggt tcgtgatcga ggaagtaaac gagcgcggcg ccaatctcct cgggtacagg
    15421 gcgcgtgaac tggttggaag gacgctcctg gacttttttg ttgaaaaaca agagaaagcg
    15481 ctgatacagg agaaggtctc gccaggaggc gcagtcacag aatttgaatg tcacctcaag
    15541 agaaaagatg gctatgtgat ccatggactg atgtcggcag ggccgcttcc cggcaggaag
    15601 gtcgtgttca ccctcgtcga catcacgggc atgaagaagg ccgaagagga gctgcgggag
    15661 agcaaagagc gataccaggg gctttacacc aatgcgcagg ttggcctggc acgggcaagc
    15721 gtcacggacg gcaggattct cgaagcgaat gatcagatgg ccaacatgtt cgggtactct
    15781 tctgtcgatg aattcatcag ggagttccgg tttccagaac attatgcgga caaaggtgtg
    15841 agagagcagc tctttgccac gctggccgat aacgacacga tctccaactt cgaggcccgt
    15901 ttcttcagga aagacggctc cattctctgg gcacggttct gggccaggat cttcccggaa
    15961 aaaggctacc tcgaagaggt gttcaccgat atcacggagg agaaactctc gcaccgcgcc
    16021 ctcgacgaga gcgaggagcg ttatcgcaaa ctcgtggaga gcctccctga ttacgtgctg
    16081 gtctttgcga atgaccggat actcttcgtg aatccgtcgg ccgcgaacac gatgggaaag
    16141 acccctgacg agatggtgag ccaccccatt tacgactatt taagccccga gtccgtagag
    16201 acaatcagga cccacagtaa aaaacgagtg gccggcgggc acgtcaaccc ctatgagatt
    16261 gacctctctt tgaacgacgg gcggtccagg acggtgctgg cggacgccac ccctatcacc
    16321 taccggaacc aggacgccat ccttgccgtg ctgaccgata tcaccgaccg gaaacgagcg
    16381 gatgagaaat tgaaagcatc caaggaccag tacatggcaa ctatcgacgc catggttgat
    16441 ggcatattcc ttgtgaacca ggaccttgaa atcgttctcg tcaactcttc attcaggagg
    16501 tggcttgagc gtattggacg acatcacgaa gtcgtcggca tgaacgcagg agacgcgctg
    16561 ccatgtgttg gcgatcccgg actaaaagcc ataaaaaacg tcttttcgac cgggaagatc
    16621 ctcgagacga ccgtagattt ccatctcaac ggaagaggct attttttcga gaaccgccat
    16681 atcccggtct ttgaaaagaa gcatgttgca cgggtaatga ccatcatgcg tgacatcacc
    16741 cggtccagga tgatcgagga ggaaaaaagg ctcgcctacg agcagatcga gaagaacatc
    16801 gagcaattcg caatactcgg cgatcacctg agaaatccca tgcaggtgat cgtgggcctc
    16861 gcggacctcg agggagggcc tcttgcagag cagatccacc gacagaccga ggagattgac
    16921 aggacagtct cccagcttga caggggctgg atagagtcag agaagatacg tgagttcatc
    16981 cgcaaatatt acgggatagg gaagtgatct cccggggacc ttcccgcgat cattcatctc
    17041 gccccaaaca ggggtgtaac agtctcaaaa gtcatttctc cgttgcgagt cctatcgggg
    17101 gcttccacca acccggattc acaagaaatc tcccttctgg ggagaactcc ggggtaaaag
    17161 gcttatcttc ccggccgatc ttatctaaca gtacgggtga acctgtgcag gtatccatga
    17221 aactggaaat taaggatgcc ccggggcagc tggtggcagc cctcaagccc atttcggagg
    17281 ttggaggcaa catcatcgcc gtgattcatc aacgcgacga gaaacagcgt ggcggctttt
    17341 tgagcgtcca gatcgtgctg gaacttcctg aaaacaggct gaaggacctg acccggctca
    17401 ttcgggagca gggagtcaac atccagcgta tcgggcacga acgcctcctg taccaaaagg
    17461 cggtaatggt gataggccac ctgatccata ccgatcttgg ggataccgtt gacaggatcg
    17521 accgcacagg gtttgccgag gtgaccgaac tgaacatcag catgccggca atttcacagg
    17581 tctcatccgc ccggctcctg ataaaatcag tgaacaagga agatatggag caggcaatgg
    17641 caatccttcg cagggtggca aaggagaagg agttccttct cgtcgagccc ctggaggagg
    17701 tagaatgaga gtcgcactgc tcggcatggg ttcggttggc cgcggggtcc tccagatgat
    17761 cggggagaag gaccttggca tcacggtcac ggcagttgcg gattccagga gtgcactcat
    17821 cgacagggac ggcatcagta tcgaggaggt actggctgca aaggcaagga acggtttttg
    17881 tggcgacaag gccatcagtg caatggatgt cgtgacaggg tcggcgtatg acgtgctggt
    17941 tgaggtgacc ccgacgaatg caactacggg ggagccagcc ctcacccata tgctctgcgc
    18001 actggagagg ggccgccacg tggtcacgtc gaacaagggc cccatttcaa gggagttttc
    18061 ccgcctccgg gagatcgccg tggagaaggg catacagctt cggtatgagg caacggtggg
    18121 tggcgccatc cccatcatgc acaccctgga gcagggcctg gcaggaaaca gggtcctcgc
    18181 catcttcggt gtcctcaacg ggacctgcaa cttcatcctt accaggatgg cggcggaggg
    18241 tcttacctac gagcaggccc tgctggaggc gagggagatg gggtacgcgg aggcggaccc
    18301 cacctatgac gtgaagggta tcgatccggc tatcaagctg gtaatccttg ccaatacgtt
    18361 ctgttcgcat tcggtcagtc tcgaggacgt tgatattacg gggattgatc gcctcaccaa
    18421 tgatgccctc cgtcttgcag aagagcagca atgtaccatc aggctgattg cagaggcgat
    18481 tcctgaccgc ggaatactgc gggtggcacc aaggctcctc cctctctcgc acccgctggt
    18541 ggtcgaaggg acactcaatg ccatcacgat cgagacggac atggcagggg agatcacatt
    18601 gatcgggaaa ggcgcgggat ccgtggaaac cgcgagtgca atcatcgggg atctcctcta
    18661 catacgggat actcatgcgg ggcgtcattc aaaaacggcg tgagtacctg cacctcatga
    18721 gggcatgcac cctggagcgc ggccatttca cggtgaccga tatccagaag ggagcaggag
    18781 ttccacggag caccgcccag gactggatca accgcctgct cgatgagggg tgcgtggtga
    18841 tccgggagag gaagatgggc cggaaccccg caaagtatgc ggctatcagt gcgcttccct
    18901 ccagtgcctg caggaggatc ttcaccacta tcgacgggga cagtgtcgag attcaccatg
    18961 agtgcatgag cagtgcctgt gctgcgttct gcgcgtacca ccactcccat gccaggggga
    19021 tcctccaggg cgtgcacagg gatgggaccc tcattcgaga atgggcccgc cttgggcgcg
    19081 aggacatcga tatcgggctt ttcccttcgt ctgcagtagg ggtcgcagga gtggagaggc
    19141 ggggggagga catcatccag cacatccgtt gcatcggggg gccggcatac tctctcaccg
    19201 acatgatgtc ccatgcggag ggcgtatgcg aggtgagcat acagcgatca ggggaggtcg
    19261 tggaaggcag cgtccggacc cgtgccctgg cccacctgat catcgggatc gatgacaccg
    19321 acagccggga gggtggagcc acgtttgccc tcgcccttgc gctcctccag catctcgaga
    19381 cactgaaggg cgttctcccc atcagccacc atgtggtcat gctcaaccct gcggtgagtg
    19441 agaagacggc ggggaactcg tgcagttata tcgaggtcgc gatcccgccc ggcatgtact
    19501 cacgggtacg tgacaggtcc ctggtctttg tcgaggacga agccctctcc cgggaatggg
    19561 gaatggcttt caagcagggg ttccaggtcc cccagagcct tcgtgcctac ggcgatatgg
    19621 caaggcgcgg tatcgttacc agggaggagg cagcagagac tgctgcgcag caccatatcg
    19681 aggtcattgg cggccggggg atcgtgggtg cccttgcggc ggtcgcactc tcgggcttat
    19741 cccacgaggt tctgctcagg gccgaagcga ggattccagg cacgagttag ccgctgaccg
    19801 taatcagcgg gataagggcc aggagaagga cgaggatcag cagaagtatc gcgacacgcc
    19861 tgagtatggc ctttgccgca gccttttccc tgtaggacct gggctgtatc tggagcgcga
    19921 ttgtcgccat gatgctcccg gccatcaggc ctatggcgtt ctggagggtg aggacaaggg
    19981 caccgagggt tccctccggg tacaatacca cggtaagacc gctcacgagc gcaggcggca
    20041 gcagtgccgc tgcaacggcg actcccgcga tactgtccgg gattcccttc gagagcgcaa
    20101 cgatggttgc aaagccgagg aatatcgcca tgatggcata tattgggctt gaatgcgtcc
    20161 tgctgagcat ctcgcctgtc acctcgagag ggatgaacaa cccgaggatc cacgtcacga
    20221 caagcgagaa gaggatgaag gaaaggatga ggaagataat attaaggata tcacggagga
    20281 cccccatggc atttcctgtc gctgagtgga gagcaaacgc gtagataggc ccgatcatcg
    20341 gggcaatcag catggcaccg atgatgatcc ccacgttgtt caaaaagagg cctaccagcc
    20401 ccacgatgct cgcaactgct gccaggctga gtatatccgc gtccagccgc tcgtaagatt
    20461 tgacggtgtc gaggagtttc tctatgggag agtgattcct cttcttcttt ttagcatcgg
    20521 gcgtctcctc cttctctttt ttctcgtcaa aaaggggata tatggtgaaa tcaggagaat
    20581 atacctcgac aagcgtgttc ttgtccatgt agccgagcgt caccttgatc tttgccaccg
    20641 tctcatcgag ttcatcgtca gggaccacga tggaaaaacg cgtcttctca ccaaaattct
    20701 cgcggaggtg aaaatgaccc tttaacagct gcgggatatt ggctgcatcc tcgtttccga
    20761 cggtgatcag gattttcttc atggcatggc ctcccctgaa tggtatgagc ttgagtggtg
    20821 ttcatccttc agtgatgtat acttaccgat gacaccagcc ccggcaggca gatacggata
    20881 gggggatcaa cgtcagccat cttcagcctc cggcgggttc tcttatccat acagatcctg
    20941 ttttttggta aaagaggaat aattaacagc caggcattca gacactcaca gggaggggat
    21001 cgcgagcatg cccgttgaaa agacagggaa agaaataccc gcgcaactca aggcgataat
    21061 ctacaggttc cagcgggatg aagtaacaga gtaccagatc taccgcatgc ttgcaaaagc
    21121 ggttcgcgat ccccacaatg catctgtcct cttgaggatg gctgaccagg aactcgggca
    21181 ttccaggata tgggaacact acacgggaaa ggtggcagga cctgcggtcg ccagggtatg
    21241 gttttactac atcatcgccc gtctccttgg gtataccttt gcggccaagc ttatggagaa
    21301 ccgggagagt tccgccattc aggactatgg tacccttgtt ggaaaaattc ctgaagtgga
    21361 ggggatcatt gccgaagaag agcagcacga gatggaaatc ctcggcatgc ttgaggagga
    21421 cagactccgg tacgtgggat cgatcgtgct tggattaaac gatgccctgg tggagttcac
    21481 ggggagcctt gccggattca cgtttgctct ccaggatacg actgtcattg cagcagtggg
    21541 gtgtatcatg ggtgtggcag ctgcactctc catggcgtca tcagagtatc tctcacagaa
    21601 ggcggaccag acaggccggg agcctgcaaa agctgcgctc tacacggggg gcgcatatat
    21661 tttcacggtc tttctcctcg tcgtaccgtt cctcctgatg ggcaacccgt tcagcgccct
    21721 cgtcgtctct ttgatcactg cggttgccat tatcgtctgt ttcacattct atacttcggt
    21781 tgcaggcggt ctcccattct ggaggagatt tctcgagatg gttgccatat cacttggtat
    21841 tgcgggagta tcgttcctta tcggcctgct gatacggggg ctgttcaacg tcgatgtatg
    21901 aagccggctc cctcctcctg ccgggcaagg agcaatggat accgtatccg gtatctctcg
    21961 aggcagtggt caagctcata tcccacgttc tgttcaggac aaaggaagaa gaagacgacc
    22021 tgtccatccg agatccatga gggaagaatc ccggtatgct cgaagagcga tgcacacctg
    22081 tgctcgatat aggcaatccg gggcgtcctt ggaatgtgct ggtaaatcat cagcagggaa
    22141 cccggtccga gggagcgggc gataagactg acttcggaat agaggaggtg tccggcaccc
    22201 ggactggcga cctcgaggcc agtgtcggga tccaggaata cgagggtgtg cgggagtcca
    22261 tggacttttt tggcaatgcg cgagaaatat gcccctctgc cgcggtgagt gaaacgcgcc
    22321 ctgtaaaagc tgacctcgca cgatttctcc tgaaaatagt cgcgtaccat gaagcagtcc
    22381 cgggatttgc gcatgcgaag cttcgagagg aaatctgcaa gaccggcatt cagattcccg
    22441 gctcttgtac gaaaatatcc tgttctccgg ccgtgccctg acccgtcatc cggggtgagc
    22501 atgggaatga agaaagcctg tgtgagggat gggagagtgg aaagcagcgt ctcgatgaga
    22561 tcgtacttga agaggtcccg tgtatcaccc aggtactggt gcttcatcgg gaagagttct
    22621 cttgcgggac gggaaagaaa ccttccggtg ttttcatgaa gatgaggaga aaagatgacg
    22681 gatacagggt cccacccgga tggggggaca ggcatattcc tgcagccgcc ggatcacccg
    22741 gtctttaact tatagagggg ttttcgtgca tccttgaaat tgaattttgc cacaatcagt
    22801 ccgctctgcc tgagcttgtt taaggcattg cgtatggtcc gtggtgaaaa tgccaccctg
    22861 ccgatgatct cgttcaacgt ccgctctttt ccatcgccga gcagcttgaa tactgcctgt
    22921 gcagaggagg ggaggcagtc gatgctgttg actggccccc tgatgtccgg cccattcacc
    22981 tgggctgggg ttgatgccag tgtcatgaat tttttcctgt gttcatgaga ggttcgatag
    23041 tcgttctctc cgggatgtct tcccacatta ttcgcgggaa tataatccct ggatgcgtgc
    23101 cggttgcccg ggtcatgggc gggtggcact cacttcgttc gggtgtatac gaacattcta
    23161 ctatatacga actataatta tatatatttt tgcccccact gagatgtgag aactgacgcc
    23221 atgtccactc cccttcgcaa tcaggacagt cccgggaggt atatacagat cgtagcctcc
    23281 gatcaaggcc tcacgtcaat tgacagccag ctgaagggaa agatcctcga gatgcttcag
    23341 gagaaggaca tggattttga agagattgtc gccaggtccg gaagggcgaa atcgacaata
    23401 tctgcccaca tgaaagccct ctctcttgca ggtatcacca ccaccaggcc cgatccgagg
    23461 gatgcgagga aacggatatt ctccctgaac gggcgggtcc ttgtccgggc tcacacggat
    23521 gataccgaac tgctctgggc cgatcgattc tttcctgatg cgcttcccgg cgatgcaacc
    23581 acgagagatg tcttccgttt catccttaca tcgcttcgcg tgagccttct tgcggaaggg
    23641 atcagcatat acccgatact cgagagggcc gggcggaaag ccggcgccgc catttacccc
    23701 caggtcaggg ctgcggaact ctctgtcttc ctcgggaatc tctcggcgtt ctgggcccgt
    23761 ttcggactgg gggagatgga ggttgagcgg gaggaccccc tgactatcgt ggtcagagac
    23821 tgcttcgagt gcatggacct ccccatcaca gggaggcccg agtgtgcatt cgatacgggg
    23881 cttcttgcaa ccctgttctc cgcatattat ggcgagatgc atgaggtcgt cgagacccac
    23941 tgctatgcca cggggagcaa cttctgcagg tttgaggtct ggaacgcccg ttgagtggcg
    24001 ttttatccct tacctgtcct tggaatgcca ttcctgaatc atgcctgggg aaagcagcca
    24061 gaggagttct ccgcttcccg ggtctccatg aaaccgtaca cgcgcgatgc cgcctttttt
    24121 taagacaata ttgcattcat gccggctgca gatgaggaga gagacgagtg aagagagctg
    24181 aggctcgtgg cctaccagca ggatcctgtt ctcgggcatg atctccctga tcttctgtac
    24241 gaccgttccc ggcgggggac cgatggccag ttcgtcccag aatgtaaccc gatcaggatc
    24301 tcccagtgct cttgcgatga tcgacgcagt ctctgcggcc cgcctgagcg ggctcgatgc
    24361 gattgttgag aactcgatct cccgtgcctt catccaggcg cctgccaagg agatttcctc
    24421 gcgccccggt ttcgaaagac acctctcgct gtcccgggga tcgctatgat cggcgactcc
    24481 cgctttgccg tgtctcatga tatagaggtc catgagagga gaggagtata gatgattaaa
    24541 aaaacattcg ggttcccggg atgcaagggg gaatgcgttt gagatatcgc aggatgatga
    24601 gtggaccttg caaggggatg tgctgctggc cggacattgc aggatcgaga tggtccccct
    24661 gcgtgagata cgccgcggca acgagagagg ccccgtatta cgacaattgc gggggatcga
    24721 taccccggaa tccatctcgt gccgttatcc ggaaaatcac tcttttcttg tgttcccgga
    24781 ccacatacaa attgacgtga attcggaagg atcccgggaa aaaagatata aagcgcagaa
    24841 cccgaaatgc cgatgcgagg gaggggattc gaacccccga actcctgcga gaatggaccc
    24901 taaatccatc gcctttgacc gctcggcaac cctcgctctc ct
//
LOCUS       DAOQYR010000022        24666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_2356_length_24666_cov_8.789525, whole genome shotgun sequence.
ACCESSION   DAOQYR010000022 DAOQYR010000000
VERSION     DAOQYR010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 24666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24666
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2356_length_24666_cov_8.789525"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PKM87_04375"
     CDS             complement(<1..572)
                     /locus_tag="PKM87_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HNQ29336.1"
                     /translation="MDQEEDRNAIYQEALALHEEHHGKLEIRSRVPLETRRDLSRAYT
                     PGVAEVCRAIQKDRDMAYRYTLKRNSVAIITDGTAVLGLGNIGGYAAIPVMEGKAILF
                     KKFAGIDAFPICFDGPQSDFVDQVRNIAPVFGGINLEDIAAPRCFEVEEGLQDLGIPV
                     MHDDQHGTAITVLAALLNACRATKKQFEDL"
     gene            714..1610
                     /locus_tag="PKM87_04380"
     CDS             714..1610
                     /locus_tag="PKM87_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNQ29337.1"
                     /translation="MLLGIALGALLAAAGCTVPVNVTLDPSGHPAYMCGVPVDARVTR
                     VIDGDTIAVKIAGGREETVRVLGIDTPETEEHGNWGNEYEGISNPSYLTSWGHRASNQ
                     TESLVKGKSVTLMADCMTGERDRYGRLLAYVGIEGSDLGSLLLQGGYARVYTEESFGK
                     KQLYLLIQSEAQLAGTGLWSAAKTPESSQKSPVSIASVQYDAPGDDRENPNGEYIVLA
                     SEETVNLSGWTIADNSGTVYTFGDVVIPAGDRVTLYIGSGTPTGTRLYWNLSAPLLGN
                     DADAVTLRDPEGNAVAVYRWGP"
     gene            complement(1681..2661)
                     /locus_tag="PKM87_04385"
     CDS             complement(1681..2661)
                     /locus_tag="PKM87_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNQ29338.1"
                     /translation="MSHATGMEDVSVLDASTLAKKEKTVFVSLVVDFILWIPDIIAAI
                     LAGSITMFADVVKCGNELLATFFAYLTIRKLSRGGKGVYDYGMGKFETVTSIITGAVM
                     LISLVLVFYTAITRILAPEALHISGVALALVMMSIGVTVNTWLWFKNYRIAQKEYSPI
                     MESQWRLFRTKAFSDLSVLLALIFSIVLHQFWWAVYIDPFASMIIVGFLFTTGYRVIS
                     ASLPDLLDKTLDESLQITIIRELTEFFCEYEAFHGVRSRRSGSNVYIEIFLEFDGDRR
                     MCEVQEAIDRMKASLESKIPKSSVTIVPSSGSCMIPSKREPQPSANEGGV"
     gene            2860..3192
                     /locus_tag="PKM87_04390"
     CDS             2860..3192
                     /locus_tag="PKM87_04390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNQ29339.1"
                     /translation="MELQCENRGEIRVVHVAGRIDSSNAEEFGRDIRGVITGGSRQLL
                     LDFAGVPYINSAGLRAVLVAAKSLKMPGDRCVICGLSPEVKEIFELAGFMNILRTYPS
                     VEDAISHW"
     gene            complement(3362..5347)
                     /locus_tag="PKM87_04395"
     CDS             complement(3362..5347)
                     /locus_tag="PKM87_04395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B DNA-directed DNA polymerase"
                     /protein_id="HNQ29340.1"
                     /translation="MWILDTCSRNGQVEIWERGRGGRHHVVPAPSSFLLHLPDPHAHG
                     DLLEGLESRFPVEECSIRTVYGTLDGYRVQAGREAAALVEKQTRYEARIFNADLRPDQ
                     CVLAAGGLVPCSDEGESRFSTDLSFDLPAMEIAVADNPFARDPVTTLSLSVDGRQVWV
                     GGDERTILSDMAGLVRSVDPDVILFSQADIWVPRLAESARSHGLDTGLSRTGRFRRLQ
                     AKSYWSYGKTEFRVGALIPDGRLLVDTRQSFHYRESGLSGILLGARLSGLSPNLAARF
                     TSGTLISSYEVYEALRRGIAVPWRKDDPEVVRRFPELKAADRGGMMFQPVPGIYTGVH
                     EIDFTSLYPSLIVRDNLSPETLADPVKEGFLPCVLAPLIAMRMETKQKKARDPRYAGL
                     DAILKWMLVTCFGYTGYRNAKFGRIEVHEAITGSAREVLIRTRDIAEEMGFSVLHGIV
                     DCLWVQGGPVAAFKERVERETRLHTVVDAYDWLVFLPMADGTGAYTRYFGRLSDGSLK
                     VRGIAARRGDTPPFIRDVQEEMLARMQQARTPEEIAALREPLRERYRRAMEELPSARP
                     AEMAVSRRISTLRYRHACAEASAVQACAAEGIALSPGMEVSFVVTDSRTWAVDLDWKA
                     ARFDVGYYRKLLGKAWEEVIFGADKAAMREKRGEQVT"
     gene            complement(5334..5576)
                     /locus_tag="PKM87_04400"
     CDS             complement(5334..5576)
                     /locus_tag="PKM87_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29341.1"
                     /translation="MGRSFASVRMGVREVLSRWERAARALPGEDREHALRVVAMARVH
                     ASECFYAFGDPLEATLFSVLLEVAKEQEGGRRRVDP"
     gene            complement(5578..6108)
                     /locus_tag="PKM87_04405"
     CDS             complement(5578..6108)
                     /locus_tag="PKM87_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29342.1"
                     /translation="MEFDLHPAVTLRSGTFSAVVAPGEVVLAGLNDNPVLRRFLFLFV
                     SGNCSRLLSGINRRSVSIEVRRAFTAFQLLQIIRESYHTIVFVEHDPSLYEGAGEEMA
                     PQVGEGLRQLGHGSLVVLYAEFPDPSFEAMARLADRVFYLASPAARPPAPRARGRGRE
                     RYPGGLPRGQQTLGGV"
     gene            complement(6388..7212)
                     /locus_tag="PKM87_04410"
     CDS             complement(6388..7212)
                     /locus_tag="PKM87_04410"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ29343.1"
                     /translation="MQVTNYRFPPLILVLTALLLIAFPASAQTAGTYAAEGDELSDQG
                     KFEDALVKYDLAITMEPKLAFAWCGKGLALYNLTRYDEALDAFDQAIAINPNYAKAQY
                     GRGLVLYELGRYDDAIASYDIALSIYPEYAYLVYYGKAQAYAARGDYSQAIPLFDQAL
                     TLQPSKYPGVWVEKGDAMAASGDSQGALAAYQNALALEPGFALAEAGVAAVQGDKSGG
                     VVVTTAIPQATGTPSPQTTTVHTTAPAATRTKAPLSSLTCGAGLLLAAGLALYRRH"
     gene            7719..8138
                     /locus_tag="PKM87_04415"
     CDS             7719..8138
                     /locus_tag="PKM87_04415"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNQ29344.1"
                     /translation="MASRLMEYFNRQPRIGTLSTSGKDGKVDVAVFGSPHMTDGKTVI
                     MGLGKNRSLSNLMENPHAVYMIMEPGKSIMDWKGIRVYLVAKDIATSGPMLNDLKNQI
                     AKIAGEAAAGMIHAAARFEATEVRPLVDMGQGWEKSI"
     gene            complement(8290..8730)
                     /locus_tag="PKM87_04420"
     CDS             complement(8290..8730)
                     /locus_tag="PKM87_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29345.1"
                     /translation="MKRFVCVIMVLAIAVLACGCTTPAPSSQAATEAAAVPDLIGNWA
                     GTMKGFIEEKGYTDYPDEAMTMRVTEQRGRIFSGEIVFSNQTATHVVKAFAGAINPDG
                     RTFSIVEHGGGSSTGTLLSPGELELVYIERTVPFTIAIDTLKKS"
     gene            8940..11882
                     /locus_tag="PKM87_04425"
     CDS             8940..11882
                     /locus_tag="PKM87_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNQ29346.1"
                     /translation="MASHIHRIEVRYRDDPRQKTRTDRIRSLGFPVDAVHLVDVYTIA
                     TRARDFSHAELSEIAAQLVNPVVQEYTVDQATNAAFDSAIEVGFLPGVTDNVGTTARQ
                     TIEDFSGYRFGEGEAVFSSQLYFVCGALSPESVRGLSTTLANPLVNRVHVKTRDEYGE
                     KGMDPVAPFVLLHELPTAESVNLELDDKELARLGKEGIRDPLTEERRGPLALDLPELH
                     AIRDYFRSLGRRPTDVELESLAQTWSEHCKHTIFASAMDDDVPEGLYRTCIQAATNAI
                     RKKKGDRDICVTVFTDNSGAIVFDDTFLVTHKVETHNSPSALDPFGGALTGIVGVNRD
                     TIGFGLGAKPCINVYGYCVGDPGTEPALFRGKDRERPILSPRRILDGVVHGVGVGGNC
                     SGIPTPQGWCYFHDRYVGKPLVFAGTVGVMPRVHGDEKLYEKRALPGDLIVMVGGRVG
                     KDGIHGATFSSEALDPASPVTAVQIGDPITQKKFSDVIVKEARDQGLYRSITDNGAGG
                     LSCAVAEMAKESNGCHVFLDRVPLKYPGMAPWEIWISESQERMTLAVPEERLDAFMDL
                     MQRREVEATVIGRFTSSGKCVVECHGAVVMDIGLDFLHDGLPKKHLKTTPVVARNPEP
                     AAPCPDRLDATLLAMLKRKNICAKEFISTQYDHTVQGGHVLGPVQGAGRVQGCATLTK
                     VVPGSDRGVGLSQGLFPIYSEIDPYRMAIAAIDLAIRGLIAIGVPLDWIALLDNFCWC
                     SSDDPERLWQLKRAAFGCYDGATAFSTPYISGKDSMFNDFSGFDEENNPVKVSVLPTL
                     LISSIGIHPDVTKAVSMDVKFAGDLVYVIGMTAEELGGSEYFMHLGSPGNRVPALDLD
                     AARERYGRLAKAIDKGLVASAFPVSHGGLLVALAKVSIAGRLGMEIAIPSTIRPDYYL
                     FSESLGRIVVTVSPGSRQAFEETIGSDAALLGRVGGPDLRVTAGRPIVDLRVADMEAA
                     YRAPFGGY"
     gene            11885..12751
                     /locus_tag="PKM87_04430"
     CDS             11885..12751
                     /locus_tag="PKM87_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNQ29347.1"
                     /translation="MASIHADHVKPFGGGVPGRTSTIPEKALIMSGYGINSEMETQEA
                     LYRAGMRSDIVHINDLIAGTKRLSEYRLLVFPGGFSYGDDTGAGNAYANRVRNNLWDD
                     VCEFLDGDNLVLGICNGFQILANLGLVPAFGRTPAREIALMPNRKGVLECRFVTLKPA
                     SGNLWTRGISHLYCPVSHGEGNFYCTDETLKRLESGGMIAFTYCRDDLGPANGEFPYN
                     PNGSLADIAGITSEDGKVLGMMPHPERAMEFVNLYDWPRQKERLVREGKPLPTESVNM
                     RLFWNIVDYFRG"
     gene            12760..13332
                     /locus_tag="PKM87_04435"
     CDS             12760..13332
                     /locus_tag="PKM87_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ29348.1"
                     /translation="MPPASTIRAERLVLIPATVEMLVHDRGDRRGLARLLDAVVPAAW
                     PPPLLEGETISQFIRMMADGSDPNFCSWYWVRSDRAGEVRTLIGCGGTGSWSGTPDAV
                     MIGYSVLDEFQNLGYATEAIRCIAREVFSWPGIRKIIATTYPDLKASIRVLEKVGFVP
                     AGGKEQGEGMEEGTVVYILQPPPRQGPGKG"
     gene            complement(13373..14866)
                     /locus_tag="PKM87_04440"
     CDS             complement(13373..14866)
                     /locus_tag="PKM87_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNQ29349.1"
                     /translation="MRNIHGDSIHPSFFLMILSISLAIFMSSLDGTIVNIALPTISEA
                     FSISSSTVSWVATAYLLVMAGCVLVFGKISDVIGFKKVFLSGFVIFTIGSFACGALPD
                     LFNSFPILVGSRVFQAIGGAMITAIAPAMVTAYIPMEKKGKAMGIIMTLAALATAIGP
                     TIGGALTQYTSWHWIFFINVPIGIFAVLLGARVIPASKAPGVLTGFDRYGAALIFIGL
                     ATLLFAVTEGYSLGWSSPVIIGMLIVAAVAFAAFLWHELRAADPILELRLFSYRNFLF
                     TNMIMVLVFLSFSGINYLLPFYLQYVQGYEVSTAGLILTSLSVAMMISGIIAGLLFNR
                     VGARPLCITSAIILLAGYFMIWHLGVDTAMGYIVACLSAIGFGMGMLITPVANMIMNS
                     VAKGYQGMVSSLTSLERFAPMTIGIALFNLVFLQGIQAIAGHRGVTDAAPVSLKLGVL
                     AAGFDLAFFGSFLLGIGIVVLAILARQEIHPDYLEGDEGESPLAGMM"
     gene            complement(15191..16030)
                     /locus_tag="PKM87_04445"
     CDS             complement(15191..16030)
                     /locus_tag="PKM87_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ29350.1"
                     /translation="MKHLAFLTLALLSGLLLASGCISAPPAQAPSATTPIPPLEKPHY
                     TIGIDADYPPFTYRDSTANFTGLDIEAARWIAERQGFEVGFVPVPWDRVLEALMDGHI
                     DMVYSGMTITPEREKEVAFTIPYFTVTKSVAVRSGSNVTLDDLNAGRLRVGAQAGSTG
                     ESWVKEHLVNTGILDPGQAFMYPDVLTLTDALVNGSIDASLSDTPTQQQAIAGKPLII
                     IGEIPAVEQYAVAVRKNDTHLRAVMDEGLRQLMADPHWQELLEKYGLNHFTNFSFRKP
                     SQD"
     gene            complement(16257..17060)
                     /locus_tag="PKM87_04450"
     CDS             complement(16257..17060)
                     /locus_tag="PKM87_04450"
                     /inference="COORDINATES: protein motif:HMM:NF015166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29351.1"
                     /translation="MRTMKVRYPVLPALTFLLLCGIAIAHSPSDMRLDYDHGLGTLLV
                     SVTHQVDDSSTHYVREMVIQKNGVTVDTVTYASQPSRETFTYSYPVTLEPGDVARVTA
                     RCNIGGSITREYTRPAAQGTPVTVIPTTPSLAPAPWVVHAALLLAAILLLLGVAILPT
                     YGKGIAGWYRIHTALAMVGGILALVAIAMVFGPIPSGTIRDAWDAHVALGLVVLFLLL
                     CTLGAGIYRKWAGRHTGQVRTVHLWFGRVLVVLVLANIVLGLSAVGVLG"
     gene            17355..17873
                     /locus_tag="PKM87_04455"
     CDS             17355..17873
                     /locus_tag="PKM87_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29352.1"
                     /translation="MKSKDIAIVGILLAIGAILRYFLAMLHTPLTPNMIIAFYCLAII
                     LVRPKVLEALGIGIVAGILSMLISSSIFPPANLISEPIGALVCFGLYAVLKDRIGGPA
                     VATFTTTLASGFSFAAIALLAVAPKILDKYSTVFGFILVFVPIVVITAVFNAIIVQIL
                     YYPANRVLNRGS"
     gene            17874..>18118
                     /locus_tag="PKM87_04460"
     CDS             17874..>18118
                     /locus_tag="PKM87_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ29353.1"
                     /translation="MIELENVSYCYPQGGKDAISGVSLSVRPGECIFVTGPSGAGKTT
                     LCMAAAGILEHEYGGKKGGRVTIRGRDVKEYPSLSDIS"
     assembly_gap    18119..18218
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18219..19007
                     /locus_tag="PKM87_04465"
     CDS             <18219..19007
                     /locus_tag="PKM87_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ29354.1"
                     /translation="GGRIRASGPPDQVAAEESVRRILLPDFSGIRDFSVPAPQNGNKP
                     LISVQGLEHFYGDVPALRGVNLEILPGEFIAIVGENGSGKTTLIKHFIGLLRPARGTV
                     TVDGKNAATAQVSELAHTVGLVFQNPDHMFFANTVAEEIKFGIANLGIPDPDKVLERV
                     MEKTGLSHARDLYPRWLSRGERQRLAIACVLAMQPKVIVLDEPTTGLDGRESREIMEI
                     LKHLQRDGRAIVMVTHSRQIAEECTDRIIAMEGGKIASDITRRR"
     gene            19012..19800
                     /locus_tag="PKM87_04470"
     CDS             19012..19800
                     /locus_tag="PKM87_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNQ29355.1"
                     /translation="MSEILQYIHKDGLFHRMHPGTKIFLILVVGILTILTLDIPLLCL
                     ILLAVLLLALAGKVLREVLQQMALILAMSVVFILITLITMPEGEVLGYLIPGGFLPVT
                     TGALYAGVLLTLRFAILIISFQVFVITTQPRDLVNTLEKAGVPIDYNLMFLIALRFIP
                     TLQIEARRIHEAQLARGYAPGTGFVGRIRSVAPVMVPLVSNALSRATVLGLTIDMRGY
                     RTRARTPFREERMNLLDRAVIVILAVILVAFLAGTILPFFGYAL"
     gene            19842..19979
                     /locus_tag="PKM87_04475"
     CDS             19842..19979
                     /locus_tag="PKM87_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29356.1"
                     /translation="MPTCSDCFLYTPGKGGKEGECRINGPAPPDRDADRCPSRTFRPK
                     E"
     gene            20035..20358
                     /locus_tag="PKM87_04480"
     CDS             20035..20358
                     /locus_tag="PKM87_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29357.1"
                     /translation="MKKNAICVLALLLAAILIVAPAAAGIYSPQKVSPPQFSSQRSFL
                     AGVDINNPASFQSFMATVRGMDLSPSSQPNPVLPAYLGTGVTYQNLFSSGCAGCNCCG
                     CCGCC"
     assembly_gap    20375..20474
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            21056..23101
                     /locus_tag="PKM87_04485"
     CDS             21056..23101
                     /locus_tag="PKM87_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNQ29358.1"
                     /translation="MSAIYKLMLTLISCGLLYTIASAGAPVASFSVEPSSGHAPLTVS
                     FMDTSTGSPTGWAWYFGDEDHHLTEWVEQNGSAPWYGRFGHSSVALPDGSIVLMGGGG
                     GYKNDVWRSMDQGVTWTQQNASAGWAGREFHTSVALPDGSIVLMGGYNGAYLRDVWRT
                     ADQGVTWTRQNASAPWPARAKHTSVALPDGSIILMGGFNSTYMNDIWQSDDQGVTWTC
                     INPHATWSARYGMSSVVLPDGRIALVGGMYYSSAERYSRDLWISDNAGLTWTLQSYPP
                     GQSWTPRRYHTSVALADGSILIIGGECSGYLNDVVHLVKEGTPWSVRKYLLPLDQGRY
                     HHSSAVLPDGSVILSGGQTPGGIKNDVWRFPTANKEKNPRHVYKAAGTYSVTLQVFNE
                     DGSNTTRKGSNITVIPELPAPEPVGIDPGVGQNTGKINIINLSGANFDTINTPSVRLN
                     RTGHPDILASNVTTPSSSRITCTFDLAEQPAGFWDVAVENPDGKTGVLGQGFCITQGP
                     PTASFVGSPISGNTPHKVSFTDTSTGFPTSWRWTFGDGGTSEERSPSHSYTVAGTYTV
                     ALTVQNEGGTDALIRTGYITVTAARPPVITSFAPAKMVHGKTVNVAIIGKYFQTGATA
                     KLVWGAVTIPVKIKTLTPPSKIAGSVTIPAPARGKYSLVVTNPDGGTCTRANVITIT"
     gene            23157..24131
                     /locus_tag="PKM87_04490"
     CDS             23157..24131
                     /locus_tag="PKM87_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29359.1"
                     /translation="MKFDEIRVSWFIAVIITFAITSPAWADVPVNSTRETTGISSMTS
                     LDCMGAVTQSNSLAWEQSSSSLHNPPLSPGGIYSVWFDIFGNPVFGWTADPLLAELLE
                     EPIPPGEVRYTAGYTATTLATQGTTHLTRMVDVDTSNKAGYENNIDVSTHLAFLAENP
                     FGRVTSNEDLLIDAAGTHTTAESSTLCLFATDESLFVPPFCNIVRMGSSIDLSQGSIA
                     TSARSRFVSESNMFPVTMDYSIAGEGISSHQKSTYVTGSMSAYIRAHLQEGRMAEITP
                     FNPEAPPDTPRGFIPLQSTDVTYSESTTATGAIAVFTKEMEYKSGIGW"
     gene            24461..>24666
                     /locus_tag="PKM87_04495"
     CDS             24461..>24666
                     /locus_tag="PKM87_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29360.1"
                     /translation="MRKISLWIVVITLVFAFFSMSCMAYPTVSPFGEVTIGVYAPSSP
                     FDGFSFDSHTPTSPFDDIPFGGYAP"
BASE COUNT         5160 a         7202 c         7268 g         4836 t
ORIGIN      
        1 tggaggtcct cgaactgctt cttcgtggcc cggcaggcgt tgagaagggc cgcgagtacc
       61 gtgatagcgg tgccgtgctg gtcgtcgtgc atcacaggga tgccgagatc ctgcaggccc
      121 tcctccacct cgaagcaacg gggggcggcg atatcctcca ggttgatccc cccgaagacc
      181 ggggcgatgt ttctgacctg gtccacgaag tcagactggg gaccgtcgaa acagatggga
      241 aacgcgtcaa ttccggcaaa cttcttgaag aggatggcct ttccctccat caccgggatg
      301 gcggcatacc ccccgatgtt cccgaggccg agcaccgctg tcccgtcggt gatgattgcc
      361 accgagttcc gcttgagggt gtagcggtat gccatgtccc tgtccttctg gatcgccctg
      421 cagacctctg ctacgccggg agtataggcc ctggagaggt cgcggcgggt ctcgagcggc
      481 acccttgatc gtatctccag ctttccatgg tgctcctcgt ggagcgccag cgcctcctgg
      541 tagatcgcgt tacggtcctc ttcctggtcc atgattccat caaggtcgcc tcatcacata
      601 aaacccgggt ctttcctcaa gttgcgtgaa ttcaaacccg ggcggcccgg caacacttat
      661 ttcgatgcac gacgaccaga atggctatgt ccaggaaatg cccctttcac ttcatgctcc
      721 tcggaatcgc actcggcgct ctcctcgccg ccgctgggtg cacggtgccg gtgaatgtca
      781 cgctggatcc ctcggggcac cctgcctaca tgtgcggggt accggttgat gcccgggtca
      841 caagggtcat cgacggcgac accatcgcgg tgaagattgc cgggggaagg gaggagaccg
      901 tgcgtgtgct cggtatcgac acgccggaga ccgaggagca tgggaactgg ggcaatgaat
      961 acgaggggat cagcaatccc tcctacctga catcgtgggg gcaccgggca agcaaccaga
     1021 cggagagcct ggtgaaaggg aagagcgtga cgctcatggc cgactgcatg accggcgagc
     1081 gcgaccggta cggccggctt ctcgcgtacg tcgggatcga gggatccgac cttgggtccc
     1141 tgctcctcca gggcgggtat gcgagggtct acaccgaaga atcgttcggg aagaagcagt
     1201 tgtacctcct gatccagtcc gaagcacagc ttgccggcac ggggctctgg agtgcagcaa
     1261 agaccccgga gagttcgcaa aaaagcccgg tgtcgatcgc ttctgtccag tacgatgccc
     1321 ccggcgacga cagggagaac ccaaacggcg agtatatcgt gctcgcctcc gaggaaacgg
     1381 tgaacctctc ggggtggacg atcgccgaca acagcgggac ggtctacacg tttggcgacg
     1441 tggtcatacc ggctggcgat cgcgtgacac tctacatcgg ttcaggcacc ccgaccggca
     1501 ccaggctcta ctggaacctc tccgcaccgc tgctcggaaa cgatgccgat gccgtcacgc
     1561 tcagggatcc tgaaggaaat gcggtggctg tctaccggtg gggtccgtga gcgttccccc
     1621 aaaagggagc tgaagtgtgg attccgggcg gccttccaga gggacccgcg gtacccccgc
     1681 tcacacccct ccttcattcg cagacggttg tggttccctt ttcgacggta tcatgcagga
     1741 accgctgctc gggacgatgg tgaccgagct cttcgggatc ttggactcaa gcgacgcctt
     1801 catccggtcg atcgcctcct gtacctcgca catgcgccgg tcgccgtcga attcgaggaa
     1861 gatctcgatg tacacgttgc tcccgctcct ccgggagcgg acaccgtgaa atgcttcgta
     1921 ctcgcagaag aactctgtga gttccctgat gatcgtgatc tggagcgact catcgagcgt
     1981 cttgtcgagg agatcaggga gcgacgcgga gatgacccgg tagccggtgg taaagaggaa
     2041 gccgacgatg atcatcgatg cgaagggatc gatatagacc gcccaccaga actggtgcag
     2101 cacgatagag aagatgagcg cgagcagcac cgagaggtcc gagaatgcct tggtcctgaa
     2161 gagtcgccac tgcgactcca tgatgggaga atactccttc tgggcgatgc ggtagttctt
     2221 gaaccagagc caggtattca ccgtaacacc aatactcatc atgacgagcg cgagcgccac
     2281 cccgctgata tggagggctt cgggtgcgag gatcctcgtg atcgcggtgt aaaagaccag
     2341 gaccagggag atgagcatca ccgcgccggt gatgatgctc gtcactgtct cgaattttcc
     2401 catcccgtag tcatagaccc ccttcccgcc tctcgagagc tttcgtatgg taagataggc
     2461 aaaaaacgtg gcaaggagct cgttcccgca tttcaccaca tccgcgaaca tggtgatcga
     2521 gccggcgagg atggcggcga tgatatcagg tatccagagg ataaagtcca cgacaaggct
     2581 gacaaagacc gtcttctcct tctttgcaag ggtcgacgca tcgagaaccg atacgtcctc
     2641 catcccggtc gcgtggctca tctgcagtcc ttttctcttc gtatgcgctg aaaggagaaa
     2701 atacccgcag aattgatgca gagtggggat gcaggatctt ccctgctgcc gcgcaccgga
     2761 agatccgggt cgatcgcccg ctcccgcccg ccaactttta tcgtgtgcca gattccagaa
     2821 ttttccatgc gaaaggggga ccccgccgga gagtgaacca tggaactgca atgcgagaac
     2881 aggggagaga tcagggttgt ccacgtggcg ggcaggatcg acagcagcaa cgcggaagaa
     2941 ttcggcaggg atatcagggg tgtgatcacg ggcggaagcc gccagctgct cctcgacttc
     3001 gcaggcgtcc cctacatcaa cagtgcaggt cttcgggccg tccttgttgc ggcaaagagc
     3061 ctgaagatgc ctggcgaccg ctgtgtcatc tgcgggcttt ctcctgaagt aaaagagata
     3121 ttcgaactcg cgggattcat gaacatactc aggacatatc cgtcggtcga ggacgcgatc
     3181 tcgcactggt gagagaggga gtgccgcgat ccccgtcgcg gggggagtgt gaaggggtag
     3241 tttctttccc gtgaagaccg caccagccca tggcagcctg cggccgccgg cacacctcct
     3301 gtcgccccac ccttccggca ctccggcggg aatcagccgc ccccggggct cccgggcaac
     3361 atcaggtcac ctgctccccg cgcttctccc gcatcgctgc cttgtccgca ccaaaaatta
     3421 cctcctccca ggccttcccg agcagcttcc ggtagtagcc cacgtcgaaa cgggcggcct
     3481 tccagtcaag gtcaacagcc caggtgcgtg aatcagtgac aacgaagctc acctccatcc
     3541 cgggggagag ggcgatcccc tccgccgcgc aggcctgcac cgccgatgcc tcggcgcagg
     3601 cgtggcggta gcggagggtg ctgatcctcc tgctgaccgc catctctgca gggcgggctg
     3661 aagggagctc ctccattgcc ctgcggtagc gttcccggag cggctcacgg agggctgcaa
     3721 tctcttccgg cgtgcgggcc tgctgcatac gcgccagcat ctcctcctgc acgtccctga
     3781 tgaagggagg ggtgtctcca cggcgggccg cgatccccct taccttgagg ctcccgtccg
     3841 agagcctgcc aaagtaacgg gtgtaggccc cggtcccgtc ggccatcggg aggaagacaa
     3901 gccagtcata ggcgtccacc accgtatgca ggcgggtctc cctctccacc cgttccttga
     3961 acgcggcaac cggtcctccc tgcacccaga ggcagtccac gatcccgtgc aggacagaaa
     4021 agcccatctc ctcggcgata tccctggtcc ggatgaggac ttcgcgggcg ctgccggtga
     4081 tggcctcgtg cacctcgatc ctcccgaact tcgcgttccg gtacccggtg tacccgaagc
     4141 atgtcacgag catccacttg aggatggcat cgagacccgc ataccgcggg tcgcgggcct
     4201 tcttctgctt ggtctccatc cgcatggcga tgaggggagc cagcacgcag gggaggaagc
     4261 cttctttcac cgggtctgcc agggtctcgg gggagaggtt gtccctgacg atgagggatg
     4321 ggtagaggga ggtgaagtcg atctcgtgca ccccggtata gatcccgggg accggctgga
     4381 acatcatccc tccccggtcc gccgccttca gttccgggaa ccgccgcacc acctcagggt
     4441 cgtccttcct ccatgggact gcgatacccc tgcggagcgc ctcgtagact tcgtaggacg
     4501 aaatgagggt cccggaggtg aaccgggcgg cgaggttcgg ggagagcccc gagaggcgtg
     4561 ccccgagcag gatgccggag agcccgctct cccggtagtg gaagctctgg cgggtgtcaa
     4621 ccagcagccg gccgtcgggg atcagggccc ccacccggaa ctccgtcttc ccgtagctcc
     4681 agtaggactt tgcctggagc cgccggaacc tccccgtccg ggagaggccg gtatcaagcc
     4741 cgtgactgcg ggcgctttcc gccagccggg gcacccagat atcagcctgg gaaaagagga
     4801 tcacgtccgg gtcaacggag cggacgaggc ctgccatgtc cgagaggatg gtcctctcgt
     4861 cccccccgac ccacacctgc ctcccgtcga cagagagcga gagcgtggtg actggatctc
     4921 tggcaaaagg gttgtccgcg accgcgatct ccattgctgg gagatcgaaa gagagatcgg
     4981 tggagaaacg cgactctcct tcgtcgctgc aggggacaag gccacctgct gccaggacgc
     5041 actggtccgg cctgagatcg gcattgaaga tcctcgcctc gtaccgcgtc tgcttctcta
     5101 cgagtgctgc ggcctccctg ccggcctgca cccggtaccc gtcgagggtt ccgtacacgg
     5161 tgcggatgct gcactcttcc acggggaagc ggctctccag gccctccagg agatctccgt
     5221 gggcatgcgg gtccgggagg tgaaggagga aggaggaagg agcggggacg acgtggtgcc
     5281 ggccgccgcg gcccctctcc catatctcca cctgcccgtt ccgggagcag gtgtcaagga
     5341 tccacacggc gcctcccccc ctcctgctcc tttgcgacct caaggaggac cgagaaaagc
     5401 gtggcctcga gcgggtcgcc gaatgcgtaa aagcactcgc ttgcatgcac ccgcgccatc
     5461 gcgaccaccc ggagggcgtg ctcccggtct tctccaggga gggcgcgggc ggcccgctcc
     5521 cagcgggaga ggacttcacg gacccccatc cgcacgctgg caaaactcct ccccatctca
     5581 cacccccccg agggtctgct ggccgcgggg gagcccgccg gggtagcgct ccctcccccg
     5641 ccccctcgcc ctgggcgccg gaggccgtgc cgcggggctc gccaggtaga agacccggtc
     5701 cgcgagccgc gccatcgcct cgaacgaggg atctgggaac tcggcataca gcacgacgag
     5761 cgacccatgc ccgagctgcc ggaggccctc tcccacctgg ggggccatct cctcccccgc
     5821 accctcgtag agcgaggggt cgtgctcgac gaaaacgatg gtgtggtagg actcgcggat
     5881 gatctgcagg agctgaaagg cggtgaacgc ccgccgcacc tcgatgctga ccgacctgcg
     5941 gttgatcccc gagaggagcc gtgagcagtt cccgctcacg aagaggaaca ggaaccgcct
     6001 gagcaccggg ttgtcgttta acccggccag caccacctcc cccggcgcca ccacggcaga
     6061 gaaagtcccg ctccggaggg tgaccgcggg gtgcagatcg aactccatgc ctgtgagact
     6121 ggcacctcgc acttccttaa gggtgggaga gcacgcgggg tgaaagtgga cccggcatct
     6181 tcaaataggg gggcaaaaga ccccccttct tcatcgtcgc ccctctcccg ggtcttcaat
     6241 ctgggcaaaa acggatggaa aaatttgtgt gcgtcggaag aagaagccat ccgcaaaaac
     6301 accggagaac agctgggaat ttcggtttgc ccggcgagac ggactggaca gggggtgaga
     6361 acctgcagac tggcaccgga aaaaaggtta gtgtctccgg tacagagcca ggcctgccgc
     6421 gagcaggagc ccggctccgc aagtcaagga agagagcggt gcttttgtcc tggtcgccgc
     6481 gggggctgtg gtgtgcacgg tggtcgtttg cggtgaaggg gtaccggtcg cctgagggat
     6541 ggccgtcgtc accacgacac ctccgctctt gtctccctgg accgctgcga ctcccgcttc
     6601 tgccagggca aacccgggct caagggcgag cgcgttctgg taggcggcga gcgcaccctg
     6661 tgaatctcct gacgcggcca tggcatcccc tttctcgacc catactcccg gatacttcga
     6721 aggctgcagg gtgagtgcct ggtcgaagag agggatggcc tgcgagtagt cgcccctggc
     6781 tgcgtacgcc tgggccttgc cgtaatagac caggtatgca tattcagggt agatactgag
     6841 tgcaatgtca taggaagcga tggcatcgtc ataccggcca agctcgtaga ggacgagccc
     6901 ccgcccgtac tgggcttttg cataattggg gttgatggcg atggcctggt cgaatgcatc
     6961 aagggcttcg tcataccggg tcaggttgta gagggcaagc cccttgccgc accaggcaaa
     7021 cgccagtttg ggttccatgg tgatggccag gtcatacttc acaagtgcat cctcgaactt
     7081 tccctgatca gagagctcgt caccctctgc cgcataggtc cctgcagtct gtgcagaggc
     7141 ggggaatgcg atcaggagca gggcggtgag cacgaggatg agggggggaa aacggtaatt
     7201 tgtcacttgc atggagatat ctcctttaaa tctgcgtgtg tggctcttgg tataaaggag
     7261 cataaaggtt ttggactgca ggtacacagg tggagtggcc tcgtcagatg cacgtggttt
     7321 gagagagaaa aagatattcc tgttgcagta ctccaaaaga catgagcaaa aagcggaacc
     7381 gaagcgttga accgttttcc cgcccttgta tttcggcatg gaacgattga agtgtgccaa
     7441 gtgccacagg gaattctact taaattccgg atgctccatc tgtgcccccc ctccccaggg
     7501 cccccatttc tgccccgagt gcggcggtgt actccagcgg atggccccct catacttcat
     7561 ctcccaggac aggtgggaga cagactaggg ggcctgtatc cccggaactg tcatctgtgc
     7621 ccccatctcc atggcgcgat ctcccctggc ggcacaggtg cccccccgca acttttaagc
     7681 agcgtgcctg aaaagccaga tgtaaaggag gaatgcatat ggcttcaaga ctgatggagt
     7741 acttcaacag gcagccgagg atcgggacgc tcagcacctc cggaaaagac gggaaggttg
     7801 acgtcgccgt cttcggctcg ccgcacatga ccgacgggaa gaccgtgatc atgggccttg
     7861 ggaagaaccg ctcgctctca aacctcatgg agaacccgca cgcggtctac atgatcatgg
     7921 agccgggaaa gagcatcatg gactggaaag ggatacgtgt ctacctggtg gcaaaggata
     7981 tcgccacttc ggggcctatg ctcaacgacc tcaagaacca gatcgcgaag attgcaggag
     8041 aggctgcggc agggatgatc catgccgcag cgaggtttga ggcgaccgag gtacggccgc
     8101 tcgtagacat ggggcagggg tgggagaagt cgatctgaac aggtgttcct gatctcccgg
     8161 agaacctgca gcgcactcag gggatggcag cgttgcaagc cgcaacggag tataccgctc
     8221 catgccgcat gccggcccgg gctcccgatt acagatccct ctcttcagag agagaggggg
     8281 ttctctctct caactcttct tcagcgtgtc gatcgcgata gtgaacggaa ctgtcctctc
     8341 gatgtagacg agctcgagtt cgccgggtga gagaagtgtc ccggtcgatg acccaccccc
     8401 atgctcgact atggagaagg tccggccatc aggattgatc gctcctgcaa atgccttgac
     8461 cacgtgggtg gcggtctggt tcgagaagac gatctctccc gagaatatcc ttcctctctg
     8521 ctcggtgacc cgcatggtca tcgcctcgtc cgggtagtcg gtgtatcctt tctcctcgat
     8581 gaaccccttc attgtgcctg cccagttccc gatgaggtcc ggtaccgctg ctgcctctgt
     8641 tgcggcctgg gaagagggcg cgggggtcgt gcacccgcag gcaaggaccg cgattgcgag
     8701 aaccatgatt acacacacga atcgtttcat acacttcctg ttgacgtgcg aagagaaaaa
     8761 cccggatact tcggtattat aaaaaaatcc ggatcgaacc ggatctccgt ggaatgggtt
     8821 ccccatcgga tatgagtggg acgtgcaagc tgggtcaatg ggctgggccg gatgacgcag
     8881 atgcacgagg cccgcttgcg tcagtagcta ttttgtcggt ttacgcccca atattatcca
     8941 tggcttctca catccaccgg atagaagtcc gctaccggga cgacccgcgg caaaagaccc
     9001 ggaccgatcg gatccgctcc cttgggtttc ccgtcgatgc agtgcacctg gtggatgtct
     9061 ataccatcgc cacccgcgca agggactttt cgcacgccga actctcggag atagccgccc
     9121 agctggtcaa ccccgtcgtg caggagtata ccgtcgacca ggcgaccaat gcggcctttg
     9181 actctgcgat cgaggtcgga ttcctgcccg gcgttaccga caacgtgggc acgaccgcac
     9241 ggcagaccat cgaggacttt tccggatacc ggttcggcga gggcgaggca gtcttctcct
     9301 cgcaactcta ctttgtctgc ggagcactct cccccgaatc cgtcaggggg ctttcgacca
     9361 cgcttgcaaa ccccctcgtg aaccgcgtgc acgtgaagac acgggacgag tacggggaga
     9421 agggcatgga tcctgtcgca ccattcgtct tgctgcacga gctgccgact gccgagtccg
     9481 tcaacctcga actcgacgat aaggagctcg cgaggcttgg aaaggaggga atccgggatc
     9541 ccctcacaga agagcggcgc gggcccctcg cactcgacct ccccgagctc catgcaatac
     9601 gggactattt caggagcctc gggcgcaggc ctacggacgt cgagctcgag tcgctcgcgc
     9661 agacatggag cgagcactgc aagcacacca tcttcgcctc ggcaatggac gacgacgtcc
     9721 cggaggggct ctaccggacc tgcatccagg cggcaaccaa tgccatccgg aaaaagaagg
     9781 gagaccggga tatctgcgtg acggtgttca ccgacaactc cggggcaatt gtctttgatg
     9841 acaccttcct cgtgacgcac aaggtcgaga cccacaactc cccgtctgcg ctcgacccct
     9901 tcggcggagc cctgaccggg atcgtggggg taaaccggga cacgataggg ttcggcctcg
     9961 gggcaaagcc ctgcatcaac gtgtacgggt actgtgtcgg cgaccccggg acggagcctg
    10021 cgctcttccg gggaaaagac cgggagaggc caatcctctc accccggagg atcctcgacg
    10081 gagtcgtcca cggggttggc gtggggggca actgctcggg catcccgacg ccgcaggggt
    10141 ggtgctactt ccacgaccgg tacgtgggaa agcccctcgt attcgcaggg acagtcggcg
    10201 tgatgccgcg tgtccacggc gatgagaagc tctacgagaa gcgggcactg ccaggagacc
    10261 tcattgtcat ggtcggcggc cgggtgggaa aagacggcat ccacggcgcc acgttctctt
    10321 cagaggcgct tgacccggca agcccggtga ccgcggtcca gatcggggac cccatcaccc
    10381 agaagaagtt ctcggacgtc atcgtcaagg aggcgcgtga ccaggggttg tacaggagca
    10441 tcaccgacaa cggcgccgga ggactctcct gtgccgtcgc ggagatggca aaggaatcca
    10501 atggctgcca cgtcttcctc gacagggttc cgctgaagta ccccggcatg gctccctggg
    10561 agatctggat ctcggagtcc caggagcgga tgacacttgc cgtgccggag gagcgtctgg
    10621 acgcgttcat ggacctgatg cagagacgcg aagtggaggc cacggtgatc ggcaggttca
    10681 cctcaagcgg aaagtgcgtg gtcgagtgcc acggcgcggt cgtgatggat atcgggcttg
    10741 acttcctgca cgacgggctt ccgaagaagc acctgaagac gacgcccgtc gtcgccagga
    10801 atcccgagcc cgcggcaccc tgccccgacc ggctcgacgc tacgctcctt gccatgctga
    10861 agagaaagaa catctgcgca aaggagttca tctccaccca gtacgaccac accgtccagg
    10921 ggggacatgt gcttgggccc gtgcaggggg cggggagggt ccaggggtgt gccacgctca
    10981 cgaaggtcgt gccgggatcg gaccggggtg tcgggctctc gcaggggctc ttccccatat
    11041 actcggagat agacccctat cggatggcaa tcgcggccat cgacctggct atacggggac
    11101 tcatcgcgat cggcgtgccg cttgactgga tcgccctcct cgacaacttc tgctggtgct
    11161 cttccgatga cccggaaagg ctctggcagc tgaagcgggc agccttcggg tgttacgacg
    11221 gcgccaccgc gttctcgact ccctacatct ccgggaaaga cagcatgttc aacgacttct
    11281 cgggattcga tgaagagaac aaccccgtga aggtctcggt cctccctacg ctcctcatct
    11341 cctcgattgg catccacccc gacgtcacga aggcggtctc gatggatgtg aagttcgcag
    11401 gagacctcgt ctacgtcatc gggatgactg cggaggaact cggggggtcc gaatacttca
    11461 tgcacctcgg gagcccgggg aaccgtgtcc cggcccttga cctggacgcg gcgagggaaa
    11521 ggtatgggcg gctggcaaag gcgatagaca agggactcgt cgcgtccgcg ttcccggtca
    11581 gccacggagg ccttctcgtc gcactggcaa aggtctccat cgcagggaga cttggaatgg
    11641 agatcgccat cccctcaacc atccgcccgg attactacct cttctcggag tcgcttgggc
    11701 ggatcgtggt caccgtctcc ccggggagcc ggcaggcatt cgaggagacc atcggaagcg
    11761 acgcggcgct cctcggccgc gtggggggcc cggacctgcg ggtgactgca ggaagaccga
    11821 tcgtcgacct tcgggtcgca gatatggagg ccgcctacag ggcgccgttc ggggggtact
    11881 gaccatggca tccatccacg cagatcatgt gaagcctttt ggagggggcg tgcccggccg
    11941 gacatcgacg atccccgaga aggcgctcat catgagcggg tacgggatca actccgagat
    12001 ggagacccag gaagctctgt atcgggcggg gatgcggtcg gatatcgtgc atataaacga
    12061 cctcatcgcg ggaacgaagc ggttgtcgga gtaccggctg ctggtcttcc cgggcggctt
    12121 ctcctatggc gacgacacgg gtgcaggcaa cgcctacgcc aaccgggtgc gtaataacct
    12181 ctgggacgac gtctgcgagt tcctcgacgg ggacaacctc gtgcttggca tctgcaacgg
    12241 gttccagatc ctcgcaaacc tcggcctcgt gccggcgttc ggccgcaccc cggcgcgcga
    12301 gatcgccctc atgccgaaca ggaagggcgt gctcgagtgc cggttcgtca cgctgaagcc
    12361 cgcatccggg aacctctgga cgcgtggcat ctcccacctg tactgcccgg tctcccatgg
    12421 cgaggggaac ttttactgca cggacgagac cctgaagcgg ctcgaatcag gggggatgat
    12481 cgcattcacc tactgcaggg acgaccttgg gcctgcgaat ggagagttcc cctataaccc
    12541 gaacggctct ctcgcagaca tcgcgggcat cacctccgag gacggcaagg tgctcggcat
    12601 gatgccgcat cccgagcggg ccatggagtt cgtgaacctg tacgactggc cgcgtcaaaa
    12661 ggagcggttg gtccgggaag gaaagcccct ccccacggag tcggtcaaca tgcggctctt
    12721 ttggaacatc gtcgactatt tccggggttg agccgtccaa tgccgcctgc atccaccatc
    12781 cgggcggaac ggctcgttct catcccggca acggtcgaaa tgctggtgca cgacaggggc
    12841 gacaggagag gactcgcccg gctcctcgat gccgtcgttc ctgctgcgtg gccccctccg
    12901 ctgcttgaag gggagacgat ctctcaattc atccgcatga tggcagacgg gtcagacccg
    12961 aacttctgct catggtactg ggtgcggtca gatcgtgcag gggaagtgcg gacactcatc
    13021 gggtgcggcg gaacggggtc ctggtccggc accccggacg cggtgatgat cggatactct
    13081 gtcctcgacg agtttcagaa cctggggtat gcgaccgagg cgatccgctg catcgcaaga
    13141 gaggtcttct cgtggcccgg catccggaag ataatcgcga ccacgtatcc tgacctgaag
    13201 gcatcgatca gggtgctcga gaaagtgggg tttgtccctg ccggcggaaa ggagcaggga
    13261 gaagggatgg aggaggggac tgtggtgtat atcctgcagc caccaccccg gcagggtcct
    13321 ggaaaggggt gacctggccg gagaacccgg gaaaaagaag cagattttcc tgttacatca
    13381 tccctgccag tggcgactct ccttcatccc cttcgaggta gtcagggtgg atctcctgtc
    13441 gcgcgaggat ggcgaggacc acaataccta tgccgagcag gaacgacccg aagaacgcga
    13501 ggtcgaaccc cgcggcgagc accccgagct tgagcgacac cggcgccgca tctgtcacac
    13561 cacggtgccc tgcgatcgcc tggatgccct ggagaaagac caggttgaag agggcgatcc
    13621 cgatcgtcat cggcgcaaac cgctccaggc tcgtgagact cgagaccatc ccctggtagc
    13681 ccttcgccac cgagttcatg atcatgttgg caaccggcgt gatcagcatc cccatgccaa
    13741 acccgatcgc ggacaggcat gccacgatgt agcccatggc ggtgtccacc ccaaggtgcc
    13801 atatcatgaa ataccctgcg aggaggatga tcgccgaggt gatgcagagc ggccgggcac
    13861 cgacccggtt gaagaggagc cctgcaatga tgccgcttat catcatcgca accgagagcg
    13921 aggtcaggat caacccggcg gttgatacct cgtatccctg gacgtactgg aggtagaacg
    13981 ggagcaggta gttgatcccc gagaagctca ggaataccag gaccatgatc atgttggtaa
    14041 aaaggaagtt gcggtacgaa aagagccgga gttcaaggat ggggtccgcg gcccggagtt
    14101 cgtgccacag gaaagcggcg aatgcgactg ccgcaacgat cagcatgccg atgatgacag
    14161 gcgaactcca tccaagcgag tacccctctg tcaccgcgaa gaggagtgtt gcaagcccga
    14221 tgaagatgag cgcggcacca taccggtcga acccggtcag gacacccggg gctttcgatg
    14281 ccggtatgac ccgtgcgccg agcagcacgg cgaagatccc gattggcacg ttgatgaaga
    14341 agatccagtg ccacgaggtg tactgggtca gtgcaccccc gatcgtgggg ccgatcgcgg
    14401 tcgcgagcgc ggcaagcgtc atgatgatgc ccatcgcttt tcccttcttt tccattggga
    14461 tataggcggt caccatggcc ggcgcaatcg cggtgatcat cgcacccccg atggcctgga
    14521 acacccgtga cccgacgagg atgggaaacg agttgaacag gtccgggagt gccccacagg
    14581 cgaatgaccc gatggtgaag atgacgaaac ccgaaaggaa taccttcttg aacccgatga
    14641 catccgagat cttgccgaat accaggacgc acccggccat cacgagcagg taagcggtcg
    14701 cgacccagct caccgtgctc gaagagatgg agaacgcctc ggagatggta gggagggcga
    14761 tattgacgat ggtgccgtcg agggacgaca tgaagatcgc aagggagatg gagaggatca
    14821 tcaggaagaa ggaggggtgg atggaatctc cgtgtatatt cctcattagc agatattcca
    14881 tggtggagta cattatggtt ttcgaatgga tcatggaatc cctgtcctcc catcggaaag
    14941 acgctccata ttcaagggaa gatcctccgt atcacacgga aggccacgcc gggaaaaaat
    15001 ccctttcctg ctcctattgc atcgtagcgc gatggcatgg atcgtccggg tatccccatc
    15061 tcacttgggg accaggcgcc cgaacatcat actggagaac tgcaacattc ctgcgaacac
    15121 cggaaggacc aggacccatg atatcagtca aactccagtt gaattgaaaa ccgccatttt
    15181 aatcggcact ttaatcctgc gaaggcttcc tgaaagaaaa attggtgaaa tggttgaggc
    15241 cgtacttctc caggagctcc tgccagtggg gatctgccat cagctgccgg agcccctcgt
    15301 ccatcacggc acgaaggtgc gtatcgttct tccggactgc gaccgcgtac tgttcaacag
    15361 ccggtatctc tccgatgatg ataaggggtt tccctgcaat cgcctgctgc tgggtaggag
    15421 tatccgatag agaagcatcg atgctcccgt tcacgagagc gtcagtgagg gtaaggacgt
    15481 cggggtacat gaacgcctgc cctgggtcga ggatgccggt attcacgagg tgttctttca
    15541 cccaggactc gcctgtcgat cccgcctggg cgcctaccct caaccggccc gcattcaggt
    15601 cgtcgagggt gacattcgag cctgaacgaa cggcaacgct cttcgtgacg gtgaagtacg
    15661 gtatggtgaa ggcgacctcc ttttcgcgct ccggagtgat cgtcatcccg gaatagacca
    15721 tgtcgatatg gccgtccatg agtgcttcaa gtaccctgtc ccaggggact ggaacaaacc
    15781 cgacctcgaa accctgcctt tccgcgatcc accgggcagc ctcgatgtca aggcctgtga
    15841 agtttgcggt actgtcccga taggtgaacg gcggataatc cgcatcgatt ccgatggtgt
    15901 aatgcggttt ctccaatggc ggtattggtg tggttgctga cggggcctgg gcgggtggtg
    15961 cagatatgca ccctgatgcg agcagcaggc cagagaggag ggcgagggtg agaaaggcga
    16021 gatgcttcat acaatacgca gggattacag gggaattaac ggtttctcca attatttggg
    16081 atgtgttgga tgatactctg ttggataatt acatggtgtt ccgcttgcgc ctgctccatc
    16141 cataaaagag agatatctga gagagggggt tgaattaaat caggtcctct tcaacctcac
    16201 cctattttcc cgggggcctg gagcaaaggg gaggaaacac catccaggct ccagatttac
    16261 cccaacaccc ccaccgcact caatccaagg acaatattcg caaggaccag cactaccagt
    16321 acccttccga accagaggtg tacggtcctg acctggcccg tgtgcctgcc ggcccatttc
    16381 cggtatattc cggcaccaag cgtgcagagc aggaggaaaa ggacaacgag cccgagtgcc
    16441 acatgggcat cccaggcatc ccggatagtt cctgacggga ttggtccaaa gaccatcgcg
    16501 atcgccacga gtgcaaggat tcccccgacc atcgccaggg cagtgtgaat gcgataccag
    16561 cctgcaatcc cttttccata tgtcggcagg atggcaacac cgaggaggag caggattgca
    16621 gcgagaagca atgcggcatg cacgacccag ggtgcgggag ccaacgaggg cgtggtggga
    16681 atcacggtga caggcgtgcc ctgggcggcg ggcctcgtat actcgcgggt gatagagccc
    16741 ccgatattgc acctggccgt gacgcgtgcc acgtcccctg gttcaagggt caccggatag
    16801 gagtacgtga aggtctctct cgacggctgg ctcgcatagg tgaccgtgtc aaccgttact
    16861 ccgttcttct ggatcaccat ctccctgacg tagtgggtgg aggaatcgtc gacctggtgg
    16921 gtcacggaca cgagaagggt gcccaggccg tggtcgtaat cgaggcgcat gtcagaaggg
    16981 gagtgtgcaa tggcgatgcc gcagaggaga aggaaggtga gagcgggaag gacaggatac
    17041 ctgactttca ttgtgcgcat gcactaccct ctgtgacaga agtgataagg gttctccccc
    17101 gaaaagggct tatttttcaa cattttttta tacatgaatg tataaattta tacatcaaag
    17161 tacataggtg tagaaacgga aatgccctgc agtgatattg cctacgcgac tccgcgaaag
    17221 atgcagaaat cttctgtgta ctttccggtg ctggccccct ggcgtttttc cttttgacag
    17281 taccatatca ttgctcgttt gagccatctg atctgccttt cgaataccaa tctcctacca
    17341 gacgtgatgc acacatgaaa tcaaaagaca ttgccattgt tggcatattg ctggccatcg
    17401 gcgccatcct gcgctacttt ttggccatgc tgcatacccc gctgaccccg aacatgatca
    17461 tcgccttcta ctgcctggcg atcatccttg tcaggccgaa ggtgctcgaa gccctcggca
    17521 tcggcatcgt cgcagggatc ctctcgatgc tcatctcgag ctccatcttc cccccggcaa
    17581 acctcatcag cgaaccgatc ggggcactgg tctgcttcgg gctgtacgcc gtgctgaaag
    17641 accgtatcgg cggacccgcg gtcgccacgt tcacgaccac ccttgcaagc ggtttctcct
    17701 ttgccgcaat cgccctgctc gcagtcgctc caaagatcct cgacaagtac tcgacggtct
    17761 ttgggttcat cctggtcttt gtgccgattg tcgtgatcac cgcggtgttc aacgccatca
    17821 tcgtccagat cctgtattac cctgcgaaca gggtgctgaa ccgggggtcg tagatgatcg
    17881 agctcgagaa tgtcagctac tgctacccgc aggggggaaa agacgcgatt tcgggcgtct
    17941 cgctctcagt gaggccagga gagtgtatct tcgtaaccgg cccttcgggg gcgggaaaga
    18001 ccaccctctg catggcggca gcaggcatcc tcgagcatga atacggcggg aagaagggcg
    18061 gccgggtcac catccgggga agggacgtca aagagtaccc aagcctctcc gatatctcnn
    18121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg ggggagaata cgggcttccg
    18241 gcccccccga tcaggtcgca gcggaagagt ccgtgaggag gatacttctc ccggattttt
    18301 ctggaatcag ggacttttca gtccctgcgc cgcagaacgg caacaagccg ttgatatcgg
    18361 tgcaggggct tgaacacttc tacggagatg tcccggctct tcggggcgtg aaccttgaga
    18421 tcttgcctgg agagttcatc gccattgtcg gagagaatgg ctcggggaag accacgctga
    18481 tcaagcattt tatcggcctg ctccgacccg cgcgggggac cgtgaccgtt gatgggaaga
    18541 atgccgcgac cgcacaggtc tcggaacttg cccacactgt cggcctcgtc ttccagaacc
    18601 cagaccacat gttctttgcc aacaccgttg ccgaggagat caaattcggc atcgcaaacc
    18661 tcgggatccc cgaccctgac aaggtgctgg agagagtgat ggagaagacc ggcctctcac
    18721 atgcccgcga tctctatccc cggtggctct cgcgggggga gcggcagagg cttgccatcg
    18781 catgcgtgct tgcaatgcag ccgaaggtga tcgtcctcga cgagcccacc accgggctcg
    18841 acggccgcga gtcccgcgag atcatggaga tcttaaagca cctccagcgg gacggccggg
    18901 ctatcgtgat ggtcacccac agccggcaga tcgccgagga gtgcacggat cggatcatcg
    18961 cgatggaagg cgggaagatc gcatcagaca tcaccaggag gaggtgagcg agtgtccgaa
    19021 atcctccagt acattcacaa ggacgggctc ttccaccgga tgcatcccgg caccaagatc
    19081 tttttgatcc tggtcgtcgg cattcttacc atcctcactc ttgacattcc cctgctctgc
    19141 ctgatcctcc tcgcagtact tctcctggca ctggcaggaa aggtgctgcg cgaggtactc
    19201 cagcagatgg cgctcatcct ggcgatgagc gtggtattca tcctcatcac gctgatcacc
    19261 atgccggaag gcgaggtcct cgggtatctc ataccgggag gcttcctccc ggttacgacc
    19321 ggggcactgt acgcgggagt cctcctcacg ctccggtttg ccatcctcat catctccttc
    19381 caggtcttcg taatcaccac ccagccgcgt gaccttgtga acacgctcga gaaggcaggg
    19441 gttcccatcg actacaactt gatgttcttg atcgcccttc ggttcatccc gacgctgcag
    19501 atcgaggcga gaaggatcca cgaagcccag ctcgcacggg gctatgctcc cggaaccggc
    19561 tttgtcggca ggatcaggag cgtcgcaccg gtgatggtcc cgctggtctc aaacgccctc
    19621 tcaagggcaa cggtgcttgg ccttaccatc gatatgaggg gctaccgcac cagggcccgg
    19681 acacccttcc gcgaggagcg gatgaacctc ctggaccgtg cggtcatcgt catcctcgcg
    19741 gtcatcctgg tcgcattcct tgccggcacc atcctcccgt tttttgggta tgccctttga
    19801 taggcacaac atttttatcg cgctggccgc atctctctca catgccaacc tgcagcgact
    19861 gcttcctcta tacccccggg aagggtggga aagaagggga gtgccggatc aacggcccgg
    19921 ctccccctga ccgggacgca gaccggtgcc cctcgcggac cttccggcca aaagaataac
    19981 gcgtggcact ctacccgggt aacattgaaa taatgcgatt gagtactctt attcatgaag
    20041 aagaatgcca tctgtgtact ggctctcctg ctcgcggcga ttcttatcgt ggctcccgca
    20101 gctgccggta tctactctcc ccaaaaagtg tcacccccac aattctcctc gcagagatcc
    20161 ttccttgccg gggtggacat caataacccc gcatcattcc agtcgttcat ggccacggta
    20221 agggggatgg atctttcgcc atcatcacaa cccaatccgg tgctgccagc ataccttgga
    20281 acgggtgtga cctaccagaa ccttttcagc agcggatgtg ccggctgcaa ctgttgcggc
    20341 tgctgcggtt gctgctgatt cttcgcatct ctttnnnnnn nnnnnnnnnn nnnnnnnnnn
    20401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20461 nnnnnnnnnn nnnngccggc tgcaactgtt gcggctgctg cggttgctgc tgattcttcg
    20521 catctcttta tttctaattt ttttacttct attcccgaag gacgcacttt tgtattccaa
    20581 tctgtcgatg aacgtagaga tgatccattc aatccccaca catgatctga agcgattgtt
    20641 gtatcgataa aggggagtcc ggagaccgac tggaaaatgc atgaaatttt aatcgatgag
    20701 aaagaccctt gaatttcatg gtatgaatgc actatctcca gggcaaatcg atatcttctt
    20761 accaaatcat gaaaaaataa aagagaggca tgtgaagaat attttgaaag ttaggctgca
    20821 aatatttcac tttcagataa tccggagcag gatatcggaa ttttgatatt actgataatt
    20881 tcatccaatt acaggattgc ggccacaacc cgccctattt cctcgtgtgc caggaactcc
    20941 catcgaatag atgtaaagga tccacaactt cagaacaaag aacacacaaa aacccggata
    21001 tcgcatacca cgcgctcccg atccgaatta tacccccatc tctgaaggtg aacgaatgtc
    21061 tgccatatac aaattaatgc tcactctgat ctcctgcgga ttgttataca caatagccag
    21121 cgccggagca ccagttgcct cattttctgt cgagccctca tcaggacacg ctcctctcac
    21181 tgtctcgttc atggatacgt caacaggttc cccgacgggt tgggcatggt atttcggtga
    21241 cgaggatcac cacctgactg aatgggttga gcagaatgga tctgccccat ggtatggcag
    21301 gtttggccac tcaagcgtgg cgttgcctga cgggagcatc gtactgatgg gaggcggcgg
    21361 cgggtataag aatgatgtgt ggcgctccat ggaccaggga gtcacatgga cacagcagaa
    21421 tgccagtgca ggatgggcag gacgcgagtt ccatacgagt gttgcgctcc cggacgggag
    21481 catcgtgctg atgggcggct ataatggcgc atatttgaga gatgtctgga gaactgccga
    21541 tcagggggtc acgtggacca ggcagaatgc cagtgctccc tggcctgcac gtgcaaaaca
    21601 taccagtgtc gcattgccgg atgggagcat catcctgatg gggggattta acagcacata
    21661 catgaacgac atatggcagt ctgacgacca gggtgtgacc tggacctgca tcaaccccca
    21721 tgccacctgg tcggcccgat acggaatgtc cagtgtcgtg ttgccggatg gccggatcgc
    21781 ccttgttggg gggatgtatt actcttcagc ggagagatat tccagggacc tctggatttc
    21841 ggacaatgct ggcctgacct ggaccctgca gagctatcca ccgggacagt cctggacacc
    21901 ccggcgatac cataccagcg tcgcactggc tgatggaagc attctgatca taggtggcga
    21961 gtgctcggga tacctgaacg atgtggtgca ccttgtaaaa gaaggaacgc cctggtccgt
    22021 ccggaaatat ctgttgcccc tggatcaggg acgataccac cactcaagtg ccgtgttgcc
    22081 ggatggaagt gtcatcctct cgggcggaca gacgccaggt ggtatcaaga atgatgtatg
    22141 gcgatttcca actgcgaaca aagagaagaa tccccgacat gtctacaagg ctgcaggcac
    22201 ctattccgtg acgctccagg tattcaatga agatggctcc aacaccacaa gaaaggggtc
    22261 gaatatcact gtcattcctg aactgcctgc accggaaccg gttggaattg atcctggagt
    22321 gggtcagaat acgggaaaaa tcaatatcat aaacctttcc ggggcgaatt tcgataccat
    22381 taacacccct tcggtgaggt tgaaccggac aggccatcct gatattttgg cgagtaatgt
    22441 caccacgcct tcgtcatccc ggatcacctg cacgtttgac ctggcggaac agcccgccgg
    22501 cttctgggac gtggcggtcg aaaaccccga tggaaagaca ggggttcttg ggcaggggtt
    22561 ttgcatcaca cagggacccc cgactgcatc gttcgtcggt tcaccaatat ccgggaatac
    22621 accgcataag gtctctttca ctgacacgtc gaccggtttc cctaccagct ggcgatggac
    22681 attcggggat ggaggaacaa gtgaggagag aagtccatcc cattcctata ccgtggcggg
    22741 aacctataca gtggcactga ccgtccagaa tgaaggagga acagacgcgc tcataagaac
    22801 agggtacatc acggtcactg cggcccggcc ccctgtaatc acctcgtttg cccctgcgaa
    22861 aatggtccac gggaagaccg tgaacgtagc aattatcggc aaatatttcc agaccggtgc
    22921 gaccgcgaag ctcgtttggg gggctgttac tattcctgtg aagataaaaa ccctcacacc
    22981 cccgtcaaaa atcgcggggt cggtcactat tcccgcaccg gcaaggggga agtatagtct
    23041 ggtcgttacc aatcccgatg gaggaacgtg cacgagggcg aatgtcatca cgattacctg
    23101 aaaggtgtcc taataatgaa cgcgacgatt gaagggaatc aaggatgtga aggagaatga
    23161 aattcgatga gattagggtc tcctggttta tcgcggtcat aattactttc gcaatcacca
    23221 gcccggcatg ggcagacgtc ccggtcaact caacgcggga aacaacgggc atctccagca
    23281 tgacaagcct cgactgcatg ggcgcggtca cgcaatcgaa ctcgctcgcc tgggagcagt
    23341 cgagctcatc gctccataat ccgcctcttt ctcccggcgg tatctactcg gtatggttcg
    23401 acatctttgg aaatcccgta tttggctgga ctgctgatcc attgcttgcg gagttgctgg
    23461 aagagcctat tccaccgggg gaggtccggt ataccgcagg atacactgcg actactctgg
    23521 caacgcaggg aaccactcac ctgaccagaa tggttgatgt cgatacctcg aataaggcag
    23581 gatacgagaa caatatcgat gtctccaccc acctggcgtt cctcgcagaa aacccgttcg
    23641 gacgagtcac atccaatgaa gatctcctga tcgacgccgc cggtacacat acaacggcag
    23701 agagttccac gctctgcttg ttcgcgacag atgagagcct ctttgttccc ccgttctgca
    23761 atatcgtgcg gatgggcagc agcatcgatc tctcgcaggg atctattgca acctctgcac
    23821 gctcacgatt cgtgtcggaa tcgaacatgt ttccagtgac gatggattat tcaatcgctg
    23881 gagagggcat ttcctctcac caaaaaagta cttatgtgac aggctcgatg agtgcataca
    23941 tccgtgcgca cctccaggag ggccggatgg cggaaatcac cccgttcaac ccggaagctc
    24001 cccctgacac gccgagaggg tttatcccgc tccagtccac ggacgtcact tactccgaat
    24061 ctacaactgc aacgggtgca attgcagtgt ttacgaagga gatggaatac aagtcaggga
    24121 ttggatggtg acccggagta tccttaaaaa gtgaaagatg aagagaaacg taaatttttt
    24181 tttccctgcc atttacttcc gtttttcggc caatattcac acattcccat tttacaaaga
    24241 aatggctgcg tcattttcat aaatgcgtat catgtagttt tttttagatt tcttcctgtt
    24301 ttcatccaat ccctttgcct gtataaataa aaactggacc atggaatcgc tatttcgtga
    24361 cccataccgg cgaatcctgg acctcttacg ttcacaagag gagattgatt gcgagagatc
    24421 ccagacatct atttataaga ttgcaatgag attctcatat atgagaaaga tatccctctg
    24481 gatcgttgtt atcacccttg tttttgcgtt ttttagtatg tcctgcatgg cataccccac
    24541 cgtgagtccc tttggcgagg taaccatagg ggtatacgct ccctccagtc cctttgacgg
    24601 gttctccttc gattcgcata cccccacgag ccccttcgac gatatcccgt tcggcggata
    24661 cgctcc
//
LOCUS       DAOQYR010000023        24450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_2385_length_24450_cov_9.602705, whole genome shotgun sequence.
ACCESSION   DAOQYR010000023 DAOQYR010000000
VERSION     DAOQYR010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 24450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24450
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2385_length_24450_cov_9.602705"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PKM87_04500"
     CDS             <1..188
                     /locus_tag="PKM87_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron cofactor
                     biosynthesis protein"
                     /protein_id="HNQ29361.1"
                     /translation="NLIPLIAQYKFADITPPTPEMKRKMQDECSKYVKQMRHCQRCRA
                     DAIGRLGHDVQSCMYSE"
     gene            complement(334..669)
                     /locus_tag="PKM87_04505"
     CDS             complement(334..669)
                     /locus_tag="PKM87_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29362.1"
                     /translation="MDYTRTGGIAAFDDRLVIFDNGQAVYSRRITKGEFTLPEDRLSE
                     LKSLLSDADFPSLASSYPAPSPGADYFSYTLTHDGKTVTTETGGIPDPLVAVISRLDA
                     ILADYAPLT"
     gene            complement(916..1242)
                     /locus_tag="PKM87_04510"
     CDS             complement(916..1242)
                     /locus_tag="PKM87_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29363.1"
                     /translation="MELVDTLRVFLENGDDWERKLTSIRGVTILKLPQTKSRPASLAI
                     EINPLTDKGTPMKKKGVMIMGQAELNAFREIFNNEKVGVLLSSLESLVPARKGAKGEE
                     GDVLQI"
     gene            1411..2289
                     /locus_tag="PKM87_04515"
     CDS             1411..2289
                     /locus_tag="PKM87_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HNQ29364.1"
                     /translation="MPRITLGELCPFSLDLTLGCGQVFRWQKSGEWWTGVVEQDVIRI
                     RQKGRTLIWEGAPWERIRDYFGLDHDLGRIIESVDHDPVIHLAIASSTGLRLIHQPPW
                     ECLASYICATYANIPGIRRKIALICDTFGDEIPLKEDFGLSTFPSAEILAESAPCDLL
                     QCSLGYRAPYLCATAREIAADPGWIRRIESLDYPAARRDLMRLRGVGPKVADCVLLFA
                     FRKYEAFPVDVWIARIMRRYYGAGEKEGYEQMSRRGREYFGPCAGYAQEYLYSERDRL
                     LSSELGVQVPSSQLAR"
     gene            complement(2259..3485)
                     /locus_tag="PKM87_04520"
     CDS             complement(2259..3485)
                     /locus_tag="PKM87_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNQ29365.1"
                     /translation="MSTGMQKIEWARQYMPVLEAIRERFSKEHPLRGTRIGMALHVEA
                     KTANLVATLVSGGAEVFITGCNPLSTQDDVASALNSMDGVECYAKRGASVQEYYAAID
                     RVLDAEPDVTVDDGMDLIHRIHTKRTELIPKVIGGCEETTTGVHRLRAMARDQQLMFP
                     VIAVNDTPMKRFFDNVHGTGESALTAIMATTNVLMAGKCVVVAGYGYCGRGLARKLHG
                     LGARVIITEIDPRRALEAYMDGFLVMPMDEAVRNGEIFITTTGNTSVITSRHFSMMRD
                     GAILANAGHFNVEIDVKWLEEHAGRIERRDGIDSYQVGAKKVHVLAEGRLVNLASPKG
                     MGHPVEVMDLSFALQALCVLHIAGHGRNLSPGVYPVPESIDEEVARTKLTALGLAIDS
                     LTPSQEHYLASWDEGT"
     gene            3628..4434
                     /gene="hisF"
                     /locus_tag="PKM87_04525"
     CDS             3628..4434
                     /gene="hisF"
                     /locus_tag="PKM87_04525"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNQ29366.1"
                     /translation="MVLTRRIIPCLDLKDGRVVKGTHFLSLRDAGDPVELAQRYNEQG
                     ADEVVFLDITASREHRGTMLEVIGRAADQLFLPLTVGGGIRSVDDIQQILRAGADKVS
                     INTSAVLDPMLITKGSSRFGTQCIVLAEDVRRNYSEHPEATMIPLPDGSRCWYEVVIY
                     GGSKPTGIDAVAWARDAEQRGAGEILLTSMETDGTKNGFDLPVTRAISEAVGIPVIAS
                     GGVGTLEHFYDGFVKGKADACLAASVFHYGEYTVRQVKEYLASRGIPVRL"
     gene            complement(4391..6031)
                     /gene="pheT"
                     /locus_tag="PKM87_04530"
     CDS             complement(4391..6031)
                     /gene="pheT"
                     /locus_tag="PKM87_04530"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNQ29367.1"
                     /translation="MAVITLPYRYLERLTRTDRKTILEKISLIGSDVERIEEDHADVE
                     FFPDRPDLFSPEGVARAMRGYLGIETGLPVYRVRPSGISFTVDPNLASIRPVLGAAVI
                     RGVSFDDESIQSIMSLQESLHWAVGRGRSKVAIGIHDLDTVTPPFRYIASPRSRRFVP
                     LDYREEMSMDEILEKHPKGRDYARIVRDFPLFPLIVDQDDHVLSFPPIINGERTRVTI
                     DTKNILLDTTGTDTRAVGVAVNIICTALAEAGADIESVAVGGEEIPTLAPGERSVSVK
                     ECSRLLGIELTAPQMALLLQKMRFGAEAEDTETVKVQVPCYRADIMHDWDLFEDVAIA
                     YGYDRIISQPPPTYTVGKPNAVLVRADRVREVLCGLGYLEVMPFTLTSEEIMYSRMQR
                     PEHPGVLHVMHPITIENTIVRTDLIPLLLEFLAMNRHRELPQRLFTVGDVVHHCTTHQ
                     QAAGVSTHPEADFSEAYATADALLHELSLEYTAMESADPAFIDGRRVDIMAGNRKIGM
                     FGEIHPAVLNGFELEHSVAAFEIDLRALPGYRGMPGTP"
     gene            complement(6034..7476)
                     /locus_tag="PKM87_04535"
     CDS             complement(6034..7476)
                     /locus_tag="PKM87_04535"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNQ29368.1"
                     /translation="MAELTQNEKRLLAVLGKEERTFATPLAGLLGATPEAVVQWAHLA
                     QEKGLVATERIVARELVYTDEGKRYLETGLPETQLLREIRQGSTVDDLRKHAAFAIGF
                     GQLRKKGLIRVTGSAVETIPGASTDADEAALKNPAPDDPRTKDLIKRGILAEHETVQH
                     KIAITPEGLALVQAGLDLRAETGTLTRDQIVSGTWRDVNFRRYDVKKLPRRLYPGKVH
                     PYQRIIGEMREILLTMGFEELYGEIVQQCYWNFDALFQPQDHPAREMQDTFYLSETLP
                     LPHGYEKVKAMHINGGGTSSTGWGGTWKEEKARQCVLRTHTTCLSIQHLAEHRDPPVK
                     AFAVGRVYRRETIDTTHLAEFEQLEGIVMDEDVTFANLLGILREFYNRMGLESVRFKP
                     SYYPYTEPSLDAEAYVEGIGWVEMGGAGVFREEVTAPLGITCPVLAWGLGISRIAMLR
                     LGLKDLRLLHKSDIQFLRETPVIREAEGGI"
     gene            complement(7478..8728)
                     /locus_tag="PKM87_04540"
     CDS             complement(7478..8728)
                     /locus_tag="PKM87_04540"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNQ29369.1"
                     /translation="MEEINPWSSQPLVDVEKLFTDFGIETIENVLPMIPEVPSFMRRR
                     IVIGHRDYQQIAGCIRDKRPFHVLTGFMPSGHPHLGHLMVMKEVVWHVQQGGSGYVAI
                     ADREAHAVRDISWEKCDEFGKEYLACLCALGFEGKTYFQSRNNRLKDLAFEAATKVNF
                     SELTAIYGFGADTELAHAESVITQVADILFPQVDSSPAPTVVPVGVDQDPHIRLARGI
                     AHKMRMFTIEEREGYISVRSKNAPETALNAVKSAFPHAKKYEGHVDIKGGTCAEVAAR
                     VREIERAHGGFAFYTPSSTYHIFMPGLTGGKMSSSIPESTISFHEPDPVVRKKVMNGI
                     TGGRITLEEQKRLGGEPDRCSLYLLNLFHMVTEDAELAEIRRKCLGGEITCGQCKKDT
                     AERVVAFLKEFREKIDACADTIRV"
     gene            complement(8738..9745)
                     /gene="endA"
                     /locus_tag="PKM87_04545"
     CDS             complement(8738..9745)
                     /gene="endA"
                     /locus_tag="PKM87_04545"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HNQ29370.1"
                     /translation="MKARLDGVSVRLGSEGRSLYDQSGYGRPDGSGLRLSPEEACYLL
                     SRKRIEIPGYDFDQLSAHFAKNPEFLRTFLVYRDLRERGYAVQTGPQDFRVFRRGHKP
                     GSGQSQYMVRVLSERDVVDFSLLIKETTSSTHMRKQHILAIVDDESELTYYEIKVQNL
                     PAALPEEPFPPLKGLLVGKSAFVCPREEERTRLASFGIALDDERLILSPLEIIYLARA
                     GVLTLWNEEGPVRLDDFLSLVADQDKELEQKMVVYTNLRQEGFIPRTGYKFGHHFRVY
                     SGLKPHSEMLVHAIAPGDMVPMSSISRSVRLAHSVKKKMLFAAQGTAGIQYVEFARIK
                     L"
     gene            complement(10278..11120)
                     /locus_tag="PKM87_04550"
     CDS             complement(10278..11120)
                     /locus_tag="PKM87_04550"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HNQ29371.1"
                     /translation="MVTIGVHVSIAGGISKAVGRASATGCDTFQIFSRNPRGWMFRDL
                     EPAEVVLFRQELGRSKLAPAVDHMPYLPNLATMNPDFAEKSGSTLVAELSRCGTLGIP
                     YLVTHLGHYGPEGKDAGQRRVSTLINDALDKVDNPVLLLLENTAGEKNGIGSRFEDIA
                     TVMEGIKKSSRIGFCFDTCHAFGAGYDLRSGEGLEETLNRFDETLGLKHLRLIHLNDS
                     KGDLGSGLDRHEHIGLGRIGEEGFARILSTPPLRNLPLICETPVDERRDDVGNIKKVR
                     HLAG"
     gene            complement(11437..12408)
                     /locus_tag="PKM87_04555"
     CDS             complement(11437..12408)
                     /locus_tag="PKM87_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HNQ29372.1"
                     /translation="MRVAIIEGDGIGHEVIPPAREAIHIIRPDIEFFEVEAGFDRWQR
                     GGEAITEDDIALLATADSILFGAVTTPPSRDYRSVIVRIRKELDLYANVRPVWGEGFD
                     IIVVRENTEGLYSGLEKIEHDSACTLRCITRKGSERIARYACTLARRRHDITIGHKAN
                     ILKSDVFFRDICLEEARKAAIQIKERFIDALCLDVLQHPDRYDVIVTTNMFGDILSDV
                     TAYLVGGLGLLPSGNIGTDHSLFEPVHGSAPDIAGRNIANPVAAFRSAAMLLHHIGEH
                     ASCGLVEQAIRATIARGIKTRDLGGTAGTSEFAGAVLAELEALVPRC"
     gene            complement(12405..13208)
                     /locus_tag="PKM87_04560"
     CDS             complement(12405..13208)
                     /locus_tag="PKM87_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29373.1"
                     /translation="MIFPRQCKEVGIAATRPCGDQVYFLSRYLIHDTPSGPEILEVTT
                     DPAGKGLMREVVETRVLAGPGETWLYPDKVQLHNRALLVRLASDSGKRCTIFTGIDEH
                     TTFVCDPDLSLFQTVAVYDVIPPRPSLSACIRDLEEAGLFSGLDVTFEHTLRDISQVD
                     ADIYPCRASGFDRTLDADLLLGGEKVAGCLTATQFLRECYGEGFEVTDICPLSMVAEE
                     PFIARCCRKEREGIGTHGGKFGAVVHWGASPSEIFSAVSDLLARWRAGR"
     gene            complement(13205..13675)
                     /locus_tag="PKM87_04565"
     CDS             complement(13205..13675)
                     /locus_tag="PKM87_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HNQ29374.1"
                     /translation="MKIEGHAVCLPADIDTDQIIAGRYLRTKDRSIWKRHVFEDLDPD
                     LAEKLHEAVIVAGKNFGCGSSREQAPVALKEAGVRAIVAPSFARIFFRNAINIGLPLA
                     EAEIPCSDGDMVEIDLTAGLITVSGISRDIRPLSPRMLQMLAAGGLVSHWRQKR"
     gene            complement(13672..14886)
                     /locus_tag="PKM87_04570"
     CDS             complement(13672..14886)
                     /locus_tag="PKM87_04570"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HNQ29375.1"
                     /translation="MPTLSERILAGGAGEYVDREVDRAYVHDGTGVLTYETFRALGVT
                     EVSHPSRAYVLFDHIVPANNSTTADLQHELRHFARSSGLQFSDIGGGICHQLMGEGIA
                     LPGEIIAGADSHTCTLGAFGTFATGVGATDMAAIFASGMTWFRIPDTIGLFLSGTLSG
                     GAEAKDLALTYVARLGMDGATYHALEFIGDGVSSLSMDSRMTICNLAVETGAKTGMFY
                     ADEVTRAYLLSYRHETAVQDPPDCAYSQEYTFDLEAIEPVIAVPPRVDTVRPVDELAG
                     IPLDQVFVGTCTNGRYEDLFRFASIVRGKKVKIRTIIGPASKNVLRKAAETGVLSDLI
                     TAGCTVLPPGCGPCLGAHMGVIGEGEVCLSTSNRNFRNRMGVGGEIYLSSVATAAASA
                     LKGEITAPGGVV"
     gene            complement(14890..16014)
                     /locus_tag="PKM87_04575"
     CDS             complement(14890..16014)
                     /locus_tag="PKM87_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase family protein"
                     /protein_id="HNQ29376.1"
                     /translation="MKPWNIEICDVTLRDGEQTPGVSFSCEEKQAIASKLDEIGVEVI
                     EAGFPVVSPYEKQCVKTVAGMGLDARICCLARARKGDVEAAVDCNVDMVSIFIATSDR
                     HIRHKYHKPREAVLEEALRMVDFARDHGLAVRFAAEDASRTDLEFLLEVYRGGADHGA
                     DLLSFADTVGSLVPMQMFRVMSTLVSTVDRPFCAHCHNDMGCATANTVTAAEAGAFQL
                     HTTVNGIGERAGNAALEEVLVALRMNGGVDRYDLSHLCDISMMVQAYSGISLQKTKAV
                     VGEHAFSHESGIHIAAILEDPQTYEYFLPDMVGGERRFILGKHTGKKALEHVVATLGY
                     SLSEQQICKVLDEVKELGESKCGITPDTLTALIRHVRKEQ"
     gene            complement(15995..17266)
                     /locus_tag="PKM87_04580"
     CDS             complement(15995..17266)
                     /locus_tag="PKM87_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNQ29377.1"
                     /translation="MKGFEVLASALSRSAYRCYAVPGYPVTELSAMAKAEPVIAEKVA
                     LEYAIGDSIRGKRAAVIMKNVGLNNGADLLVQATTQGLVGGVVIVAGDDPMAAGSTNA
                     MDSRYFGELAQTPVLEPDPEVCSESVEEAFSASEAFSRIAILRVTPQLVFGEAVATAV
                     PQRPGEGRIADPRLTMKGRVDQADSLLPGMFAWSARSRLNRMNGAVAGVGAASGDSRI
                     VTVYPPPPGLAGFTRINEIGRPFVAEHLAVLPPAAIPPPQTSESRGFYRTFCRNCPFT
                     PVFDIMKRKRIQAIADAGCSILALNPPYRIASASYGLGSAIGVAARSTHVALIGDYAL
                     LHSGLQSLIDVYEKRLALLCIVLENRCMGMTGGQEAPSPCKYIQWADPLAVRASATEQ
                     LEELITIPEEPVTLVVQGTCPEGRHHETVEY"
     gene            complement(17266..18237)
                     /locus_tag="PKM87_04585"
     CDS             complement(17266..18237)
                     /locus_tag="PKM87_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ29378.1"
                     /translation="MDWPELKARLLAEGTVRLTGKPALRYRSSSTAGPGAGTGGSVFF
                     SCGKGRVRLAIDQNSPLEIVHLGSGRAILKAWHLDIRGNLEPVALHCPRQAYITVSGR
                     CLYRCRYCEVPAIGAGRKTPEEIEAMVEQVRDRSDSIALTSGVLESIEEEEEYVIRVT
                     GRLQRFGLPIGVSIFPGKGTAERLHAAGVAEVKFNVEAATEQLFTDMCPGLSYRSVWD
                     ALEDSVPLFGRNHVFSNLIIGLGETDKEAECCIGNLCELGVVPVLRPLNPAGELSGFR
                     RPSAERLLRLFAFHTEALSAAGLDPCRALTMCIECTGCDLVPGRDRA"
     gene            complement(18219..19199)
                     /locus_tag="PKM87_04590"
     CDS             complement(18219..19199)
                     /locus_tag="PKM87_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29379.1"
                     /translation="MAKRRKEADACPELRISERPGTSPQGGEEDELPGNPYEIRYPGI
                     VALADEKGTVIELIEFFDCIGGAMWSRHHYVGSPLVRSARSVGASVRYLLRPGTAYLD
                     LQGSSFPAGICGAEIGDNEIAISYIGLGGGGVGASAGRSYAGGVIRHQTDPSGGGRKA
                     GATIWLPRMQRVLIGVDDTDTPDQGASWTLAHNIARAVEDETARYLSHTIVQLFPVPY
                     RTKNCVSIVCEFASANPGRLVARFHNLLRKYTLSDETGMAAFTGFDPSPLLPFAWMVK
                     KGEVRRDLLPEIEGGHLELIMEGRGIIGAVAAIPFYTRFEEALSLWTGQS"
     gene            complement(19323..20207)
                     /gene="fhcD"
                     /locus_tag="PKM87_04595"
     CDS             complement(19323..20207)
                     /gene="fhcD"
                     /locus_tag="PKM87_04595"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HNQ29380.1"
                     /translation="MEINGVPIDDTYAEAFPTWISRIIITAVNTEWARKAATEATGFA
                     TSAIGCPCEAGIESELRADQTPDGRPGVSILICAAKKKLKEQVVERLAECVLTSPTTA
                     VFDGLPQAAEKIAVKLHFFGDGYEYQKEVGGRKCWVIPIMEGEYIGEEEFGIVKGVAG
                     GNFFVMGENQMAALMGAQTAVEAINKVCGVITSFPGGVVASGSKVGSNKYKFMPASTN
                     EKYCPTIREKVPDSKVPAGIKGVYEIVIDGIDEKCVGEAMAKGIHAACMVPGVKYITA
                     GNFGGSLGPYKFELNKIL"
     gene            20852..21118
                     /locus_tag="PKM87_04600"
     CDS             20852..21118
                     /locus_tag="PKM87_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ29381.1"
                     /translation="MAFALHVNMERCTGCNNCVVACPVDALELFTIDPATNEKIYKVK
                     HGKAMVIDFKSELCAGCGVCVEACPHDVIRLAGRGSVTSEAKVG"
     gene            21152..22312
                     /locus_tag="PKM87_04605"
     CDS             21152..22312
                     /locus_tag="PKM87_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ29382.1"
                     /translation="MTLFPKFSKKRDGVNVIQEQKLLQQVNHLILNSEVCTGCGICVE
                     ACPEEAIALGLVGATRRGAVSYAAPVDIDEAKCSYCGVCVVLCPFNALTLKIDGEERL
                     PIVEKEGFPTYDMVADIDDEKCVRCTICEEVCPRDAIDRDVTEFEGTRADGKERQLAI
                     KSKTTFKVDDEKCTLCGICGVLCPAITVKHKPFTAESGKVEGEVVWDETKCDACKVCV
                     EACPEECITVEREVESDKLEGQVDIDQKMCCTCTWCSRSCPTEAITVEKLFEGDIEFF
                     PEKCPGGCSTCVEICPANAIYLPSSVPASQLKGKVEPTIAVNKDFCILCGACVNACPG
                     EDIIRLHRTGIRVKGKETDLFNKIKEKLCTPRTSKVKEETPGSVQLKMMEKA"
     gene            22324..22905
                     /gene="hdrC"
                     /locus_tag="PKM87_04610"
     CDS             22324..22905
                     /gene="hdrC"
                     /locus_tag="PKM87_04610"
                     /EC_number="1.8.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase subunit C"
                     /protein_id="HNQ29383.1"
                     /translation="MPRTKGYPEALDKKLADTRLYEDERHPEFTKTVEKLSMSIPHMC
                     YQCGTCTASCPSAPRSSYRIRKFVRRALLGLEDEALTDPDLWLCTTCYSCTDRCPRDI
                     APTDVIMAMRNLAFKRDIIPVNFLKTVQAIYTTGHGVPNNDVNRAARKKLGMNPEPPT
                     THAFPQYVKGIQKIIDYYELKKNADRIVKEREG"
     gene            22912..23802
                     /gene="hdrB"
                     /locus_tag="PKM87_04615"
     CDS             22912..23802
                     /gene="hdrB"
                     /locus_tag="PKM87_04615"
                     /EC_number="1.8.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase subunit B"
                     /protein_id="HNQ29384.1"
                     /translation="MREYAFFLGCIAPNRYPGAESASYKTAEKLGIKLLDLEGASCCP
                     APGAFGSISLEIWYAMAARNLCLAEQMKKDIALICNGCYKSIWEVNHKLKQHKELKEK
                     VNEVLKTIDMEFKGTIDVWHLAELFYDPEIVGVQKIRDSVKVSLEGTRIAVHYGCHLM
                     KPRKERHFGTTEHPTWLEEMVGALGAEPVEYRNKMLCCGAGGGVRGYDLVHSLDITNE
                     KMINLKEARVDALTEVCPFCQLQFDRGQIEIEEKFGVKYNLPVLHYCELLGLAQGMSP
                     QELALDLHGISCEPFLQKVL"
     gene            23817..>24450
                     /locus_tag="PKM87_04620"
     CDS             23817..>24450
                     /locus_tag="PKM87_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide reductase"
                     /protein_id="HNQ29385.1"
                     /translation="MAEKKTKKETASKKKETPKAAVPAKTEKAPAVKAPATEQKKARV
                     GVFICHCGTNIAGSVDIPDVQEYAKSLPNVAFADNYQYMCSTPGQSKIIAAIKENDLT
                     GIVVAACSPRLHEPTFRTATKEGGLNPFRFEMANIREQGSWVHMHDREGATDKAKDAI
                     RIAVAKASLLQDLIPKTVPVEHAAMVVGAGIAGMQAALDLANAGIKTYLIE"
BASE COUNT         5485 a         6966 c         6644 g         5355 t
ORIGIN      
        1 ttaatctcat tcccctgatc gcccagtaca aattcgctga catcaccccg cccacccctg
       61 agatgaagag aaagatgcag gatgaatgtt caaaatacgt aaaacagatg cgtcactgcc
      121 agcgatgccg ggcggatgca atcggacgtc tcggtcatga cgttcagtca tgcatgtatt
      181 cagagtagac catttctttt tatcctcccc cattttttcc tctgattcat tccactgtat
      241 tcccgaagag atgctcccct tcaccatacg acctgccata cagggtccag ggagaaagag
      301 agcgtaatga gagaaaaggg gggatttttt gcttcaggta agaggggcgt aatcagcaag
      361 gatggcatca agacggctga tcaccgcaac gagtggatcc ggtatcccgc cggtctcggt
      421 tgtgaccgtc ttcccatcgt gggtgagggt ataagaaaaa taatcagccc ccggggaagg
      481 tgccggatac gaagaggcaa gtgagggaaa atcagcatca gaaaggagac tcttcagctc
      541 tgaaagccgg tcttccggga gtgtgaattc tcctttggta attctccgtg aatagaccgc
      601 ctgaccatta tcgaaaatga caagcctgtc atcaaaggcc gcgatccccc ccgttcttgt
      661 ataatccacc aacaccttct gagtcgtatg attgtcagag ggaaccggag gtctggtgac
      721 gcatcctccg atgagactca ggaccaccag acatgaaaaa aacaggataa tccctgctcg
      781 tgaacgggtt gagagagcag agagggaatt tcttcgtctt gcatgattat tctgcattga
      841 ttctcttctt gcatctgggg gcatatatga ctattccagt gaggaagcgg gaaaaagaaa
      901 tggagtggct atcggttaaa tctggaggac atctccctcc tcacccttcg ctcccttccg
      961 ggccggcact aatgactcga gagaagagag caggaccccg accttctcgt tgttgaagat
     1021 ctccctgaac gcgttgagtt cagcctgccc catgatcatc acccctttct tcttcatcgg
     1081 cgtcccctta tctgtcaggg gattgatctc aattgcgagg gaggcaggtc tcgatttggt
     1141 ctgcgggagt ttcaggatcg taacgccccg gatggaggtc agcttccgct cccagtcatc
     1201 gccgttctcc aggaaaacac gtaaggtatc aaccagttcc ataccagaac atcagcggca
     1261 tgggattaaa aggatacgcg tgcagggtga aataaccaga ccgaaaagga tcaccgggca
     1321 tcctgtcgtt cccgttttcg tctcccgctc ctgctaagac gggagggtcc gatacctctt
     1381 attggaccag aatccatata ctctccatca atgccacgta tcacccttgg ggagctctgt
     1441 cctttctctc tcgacctgac cctcggatgc ggacaggtgt tccgatggca gaaatccggc
     1501 gaatggtgga ccggagtggt ggagcaggac gtgatcagga tccgccaaaa aggacgaacg
     1561 ctgatatggg agggcgcacc ttgggagcgg atacgtgact acttcgggct cgatcatgat
     1621 ctcggccgga taatcgagtc ggtggaccat gatccggtca tccacctcgc catcgccagc
     1681 agtacagggc ttcggctgat acaccagcca ccctgggaat gccttgcctc ctatatttgt
     1741 gccacctatg ccaatattcc cggcatcagg agaaagatcg ccctcatctg tgatacattc
     1801 ggcgatgaga tcccccttaa agaggatttt ggactctcaa cgtttccttc cgccgagata
     1861 ctggctgaat cggccccctg tgatctgctg caatgcagcc tggggtaccg ggcaccctac
     1921 ctgtgtgcaa cggcaaggga gattgccgcc gacccgggat ggattcgaag aatagagtca
     1981 ctcgattacc ctgcagcccg gcgggatctc atgcggctga ggggtgtcgg gcccaaggtc
     2041 gcagactgcg ttcttctttt tgccttccgg aaatacgagg catttcctgt cgatgtatgg
     2101 attgcccgga tcatgcggcg gtattacggg gcgggggaga aagaaggcta cgaacagatg
     2161 agcaggagag gaagggaata ctttggtccc tgtgccgggt atgcacagga atacctgtat
     2221 agtgagcggg acaggctcct ttcatcagag ctgggcgttc aggtcccttc gtcccagctc
     2281 gccaggtaat gctcctgcga aggggtaagt gagtcgatgg cgaggcccag ggcggtgagc
     2341 ttcgtccgcg caacctcttc atcgatggac tccggcaccg ggtagacgcc ggggctgaga
     2401 ttgcggccat gaccggcgat atggagcacg cagagcgcct ggagtgcaaa actcagatcc
     2461 atcacctcca ccggatgtcc catccccttc gggctggcaa ggttgacgag tctcccctcg
     2521 gcaagcacat gcaccttttt tgccccgacc tggtagctgt cgatgccatc cctccgctcg
     2581 atccggcctg cgtgctcctc gagccacttc acatcaatct cgacattgaa atggccagca
     2641 tttgcaagga tggctccatc ccgcatcatg gaaaaatggc gggaggtgat aacagacgta
     2701 ttgcctgtcg tcgtgatgaa tatctcgcca ttcctgacgg cctcgtccat gggcatcacc
     2761 aggaagccat ccatatacgc ttcaagcgca cgccgtggat cgatctcggt gatgatgacc
     2821 cgtgcaccga gcccatgcag cttccgcgcc agtccccggc cgcaatagcc gtacccggca
     2881 acaacaacac attttcccgc catcagcaca ttggtggtgg ccatgatcgc ggtgagggca
     2941 ctctccccgg taccatgcac gttgtcaaag aaccgtttca tgggggtgtc attgaccgcg
     3001 atgaccggga acatcagctg ctgatcccgg gccatcgccc tgagtcgatg gacaccggta
     3061 gttgtctcct cgcagccacc aatcaccttc gggatgagtt cggtcctttt cgtatggatc
     3121 cgatgaataa ggtccatccc atcgtcaacg gtcacatccg gctcagcatc gagcacccgg
     3181 tcaatggcag catagtattc ttggactgaa gcgccccgct tggcataaca ttccactcca
     3241 tccatcgagt tcagggcaga agcgacatca tcctgggtgg agaggggatt gcacccggtg
     3301 atgaatactt ccgcgccacc agaaacgagg gttgccacca ggtttgccgt cttggcctcg
     3361 acatgaagag ccatcccaat cctggttccc ctgaggggat gttcttttga aaagcgttcc
     3421 ctgatcgctt ccagtaccgg catgtactgc cgggcccatt caatcttctg cattcccgta
     3481 ctcattctac cccacaccag tggtgacacc agaccttgct ggtgcctccc atccgtcagt
     3541 tcctgcctat aggttcctcc gcccaccaga taaactgtta ccggtctttc aaaaggatga
     3601 tgatgaaggc ggtagagaag ctggtacatg gttctgacaa ggcgcataat tccgtgtctc
     3661 gatctcaagg atgggcgtgt ggtgaaagga acccatttcc tctcccttcg ggatgcgggc
     3721 gatcccgtgg agcttgcaca gcggtacaat gagcaggggg ctgatgaagt ggtgtttctt
     3781 gatatcaccg cttcacgtga acaccggggc acgatgcttg aggtgatcgg gcgggcggct
     3841 gaccagctct ttctccccct gaccgtggga gggggaatca ggtctgtcga tgatatccag
     3901 cagattctca gggcaggggc agacaaagtc agtatcaaca cgagtgcggt ccttgatccc
     3961 atgctgataa ccaagggatc ttcccggttt ggtacgcaat gcattgtact tgccgaagac
     4021 gtaaggagaa attattcgga acatcccgag gcaacgatga tcccgcttcc tgacggttcc
     4081 cgctgctggt atgaggtcgt gatttacggc gggagcaagc caacgggaat cgatgcagtg
     4141 gcatgggcca gggatgcaga gcagcgggga gcgggggaga tactcctcac cagcatggaa
     4201 acggatggga ccaaaaacgg ttttgacctc cctgtcacca gggccatctc cgaagcggtc
     4261 ggcatcccgg tcatcgccag tggcggtgtc ggcacgctgg agcatttcta tgatggattt
     4321 gtcaagggaa aggcagatgc ctgcctcgcg gcaagcgtct tccattacgg agaatatacg
     4381 gtacgccagg tcaaggagta cctggcatcc cgcggtatcc cggtaaggct ctgagatcga
     4441 tctcgaaggc agcaacggag tgctcaagct caaatccgtt cagcactgcg ggatggatct
     4501 ccccaaacat cccgattttt cggtttcctg ccattatatc caccctgcgc ccgtctataa
     4561 atgccgggtc agccgattcc atggcggtat actcgagcga aagctcgtgc aggagtgcat
     4621 cagccgttgc atacgcttct gaaaagtcag cttcagggtg agtgctgaca cccgcagcct
     4681 gctggtgggt ggtgcagtgg tgcacaacgt cccccacggt gaaaagacgc tgcggcagct
     4741 cccggtgccg gttcattgcc agaaactcga gcagcagcgg gatgaggtcg gtcctgacga
     4801 tggtgttctc gatggtgatg ggatgcatca catggaggac cccagggtgt tcaggccgct
     4861 gcatccgcga atacatgatc tcctcgctcg tgagggtaaa cggcatcact tcgaggtatc
     4921 caaggccgca gaggacctct ctgacgcggt ctgccctgac cagtacggcg ttcggttttc
     4981 caacggtgta tgtgggaggc ggctgggaga tgatccggtc atatccgtaa gcgatcgcca
     5041 catcctcgaa aaggtcccag tcatgcatga tatcagcccg gtagcagggg acttgcacct
     5101 tcaccgtctc tgtatcctcc gcttcagccc cgaagcgcat cttctggagg agcagggcca
     5161 tctggggggc ggtcagttca atgccgagga gacgggaaca ttcctttacg ctcactgacc
     5221 gttccccggg ggcaagggtc gggatctcct cccccccgac tgccacgctc tcgatgtccg
     5281 cccctgcttc tgccagggca gtacagatga tattcacggc aacgccgacc gcccgcgtat
     5341 cggttccagt ggtatcgagc aggatgttct tcgtgtcgat ggtaaccctt gtccgctccc
     5401 cattgatgat gggggggaac gagaggacat ggtcgtcctg gtcaacgatc agcgggaaga
     5461 gcggaaagtc cctgacaatc ctggcatagt cccgcccttt cggatgcttc tccaggatct
     5521 catccatact catctcttca cggtaatcga gcgggacaaa cctcctgctc cttggtgatg
     5581 cgatataacg gaatggaggg gtgaccgtgt cgaggtcatg aatgccgata gccaccttgc
     5641 tcctgccgcg gcccaccgcc cagtgaaggg actcctggag cgacatgatg ctctgaatcg
     5701 actcgtcatc gaacgatacc ccgcggatca cagcggctcc gagcaccgga cggattgagg
     5761 ccaggttcgg atcgaccgta aacgagatgc ctgatggcct gacccggtac acgggcagac
     5821 ccgtctcgat cccgaggtag ccccgcattg ccctggcaac accttccgga gagaagagat
     5881 cagggcggtc agggaagaat tcgacatcgg catgatcttc ttcgatcctc tccacatcgg
     5941 agccgatcag ggatatcttc tcgaggatgg tcttccgatc ggtccgggtg agccgctcga
     6001 gataccggta ggggagggtg atcactgcca tcgtcagata cccccttctg cttcccggat
     6061 gacaggtgtc tcccgcagga actggatatc actcttgtgc aggagacgca ggtcttttaa
     6121 tccgagacgg agcatcgcaa tcctgctgat gccaagaccc catgcgagca cggggcaggt
     6181 tatcccgagc ggggcggtca cttcctcgcg aaagaccccg gcccccccca tctcgaccca
     6241 gccgatccct tccacatatg cttctgcatc gaggctcggc tcggtgtaag gataatacga
     6301 gggcttgaac cggacgcttt ccagtcccat gcggttgtag aactcccgga gtatcccgag
     6361 gaggtttgcg aatgtcacgt cctcgtccat cacgatccct tcgagttgtt cgaactcggc
     6421 aaggtgcgtg gtatcgatcg tctcgcggcg gtatacacgc cccacggcga atgccttgac
     6481 aggcggatcc cggtgctcag cgaggtgctg gatcgagagg caggtggtgt gggtccggag
     6541 aacgcactgc cgagccttct cttctttcca ggtccctccc cagccggtcg atgacgtccc
     6601 gcctccattg atatgcatgg ccttcacctt ttcgtagcca tggggaaggg gaagggtctc
     6661 gctcaaataa aaggtgtcct gcatctcccg tgccgggtgg tcctgaggct ggaagagagc
     6721 atcgaagttc cagtagcatt gctggacgat ctccccatac agttcctcaa accccatcgt
     6781 gagaaggatt tcacgcatct caccgatgat ccgctggtac gggtgtacct tcccgggata
     6841 gagccgccgt ggcagcttct tcacatcata gcgcctgaaa ttaacgtccc tccatgtgcc
     6901 tgatacaatc tggtcacggg tgagggtccc ggtctctgcc cgaaggtcga gccccgcctg
     6961 caccagggcc agtccttcag gggtgatggc aatcttatgc tgcacggtct catgttccgc
     7021 caggataccc cgtttgatca gatcttttgt ccttggatca tcaggagcgg gattttttag
     7081 ggctgcttca tctgcatcag tggaggcccc cgggatcgtt tccacggcag atcccgtcac
     7141 cctgatgagg cctttcttcc ggagctgccc aaacccgatc gcgaacgcag cgtgtttgcg
     7201 gaggtcgtca accgttgatc cttgcctgat ctcgcggagg agttgtgtct cgggcaaacc
     7261 ggtctcaaga taccgcttcc cttcatcggt atagacgagt tccctcgcga cgatccgttc
     7321 ggtcgccacc agcccttttt cctgtgcaag atgtgcccat tgcacgaccg cctccggggt
     7381 ggcacccagc agtccggcaa gaggggtggc aaacgtcctc tcctcttttc caagtactgc
     7441 cagcaggcgt ttctcattct gcgtcagttc agccatgtca caccctgatc gtgtctgcgc
     7501 atgcgtcgat cttctccctg aactctttca ggaacgccac cacccgttca gcggtgtcct
     7561 tcttgcattg tccgcacgtg atctccccgc ccaggcattt gcgccttatt tctgccagtt
     7621 ctgcgtcttc cgttaccatg tggaagaggt tgaggaggta cagggagcac cggtcgggtt
     7681 caccaccgag acgcttctgc tcctcaagag ttatcctccc cccggtgatg ccattcatca
     7741 ccttctttct gacaaccggg tcaggctcat ggaacgagat agtgctttcg gggatgctcg
     7801 aggacatctt ccccccggtc aggcccggca tgaagatatg gtacgttgag gagggggtat
     7861 agaaagcaaa tcccccgtga gcccgttcga tctcacggac ccgggcagcg acttcggcgc
     7921 acgtcccccc cttgatatcg acatggcctt catacttttt cgcatgggga aatgccgatt
     7981 tgactgcatt gagcgcagtt tcaggggcat tctttgaacg gacactgatg tatccctcgc
     8041 gctcttcgat ggtaaacatg cgcatcttgt gagcgatgcc ccgtgcaagg cggatatggg
     8101 ggtcctggtc gaccccgacc gggaccacgg tcggggcagg gctgctgtcc acctggggga
     8161 agaggatatc agcaacctgg gtgatgacgc tctctgcatg ggcgagctcg gtatcagccc
     8221 cgaatccata aatagccgtg agctccgaga agttcacctt ggttgcggcc tcgaatgcaa
     8281 gatctttgag ccggttattc ctgctctgga aataggtctt accttcgaac ccgagggcgc
     8341 acaggcaggc gagatactcc ttgccgaatt cgtcacattt ctcccaggat atgtcccgca
     8401 cggcatgggc ctccctgtct gcaatggcca cgtatccgct gccaccctgc tgcacatgcc
     8461 agaccacctc cttcatcacc atcagatggc cgagatgggg atggccggag ggcatgaagc
     8521 cggtgaggac atggaacggt cttttatccc ggatacatcc tgcgatctgc tggtaatcgc
     8581 ggtgacctat cacgattctc ctgcgcatga aggaggggac ttccggtatc atgggcagga
     8641 cattttcgat tgtttcgatg ccaaaatcgg taaagagttt ctcaacatcc accagtggct
     8701 ggcttgacca gggatttatc tcttccatgc accacgctca cagtttgatc cgggcaaatt
     8761 cgacgtattg gattcccgca gtaccttgtg ccgcaaacaa catcttcttt ttcacgctgt
     8821 gggcgaggcg gactgatcgg gagatactgc tcattggaac catgtccccc ggtgcaatgg
     8881 catggaccag catctcggaa tgtggcttta atccggagta taccctgaaa tgatgcccga
     8941 atttataccc tgtccgcgga ataaatccct cctgccgtaa attggtatag actaccattt
     9001 tctgttcaag ctccttgtcc tgatcggcaa cgagagagag aaaatcgtcc agccgtactg
     9061 gtccttcttc gttccagagg gtgaggactc ccgcacgggc aaggtagatg atctcgaggg
     9121 gggagaggat caggcgttca tcatcgagcg ctatcccaaa tgatgcgaga cgtgttcgtt
     9181 cttcctcgcg agggcaaacg aatgccgact ttcccaccag cagccctttc aggggcggga
     9241 agggttcttc gggaagagcc gccggaaggt tctggacttt tatctcataa tacgtcagtt
     9301 cactttcatc gtcaacaata gccaggatgt gctgtttccg catgtgggtt gatgaagtcg
     9361 tttcctttat gaggagggag aaatccacga catcccgctc ggaaagaacc cggaccatat
     9421 actgggactg cccgctcccc ggcttgtggc ctctcctgaa gacgcgaaaa tcctggggac
     9481 cggtctggac cgcatacccc ctttcacgga gatcacggta taccagaaaa gttcgtaaaa
     9541 attctggatt tttcgcaaaa tgcgcagaaa gctggtcgaa gtcatacccg ggtatctcaa
     9601 tccgtttcct tgaaaggagg tagcatgcct cttcaggaga gagccgtaat ccactcccgt
     9661 caggccttcc atacccgctc tggtcgtaca gggagcgccc ttcacttcca agacggactg
     9721 agaccccgtc aagcctcgct ttcattggta ttgttattcg ctccccgttg taataatatg
     9781 ctcaccagat caggtaaggc ggcagtagac acagagattg atcgcctccc ttttccacga
     9841 agagtgacaa catactgccc cgaaatttgc aaccttaaaa cccgtgaatc acgattccgc
     9901 cccgaatttt ctcgtattct ataaaccggg aatcggcctc gtttccctgg aataaaccat
     9961 atgccgctat atcttacgcc ggcgtggaag gagagggagt gagagggcag acctctggtt
    10021 tccggatgtg cttcatgatg atttgaagat tttaaaatca aaggctccca ccgggagaaa
    10081 tgcaggcccg gaccttgtct tccacggggg acttatcttc cactaagagg agtgaaaaga
    10141 cacgaacagg agtgagaaga ggagtgaata aaagataccg ggagatggga gaagcggctt
    10201 tcagatcaac agggagcggt ttcacctatc caacggatct ccctccttcg ccagctcaac
    10261 agcagaaaca ccattcctca ccctgcaagg tgacggacct ttttgatatt ccccacgtca
    10321 tcccttctct catcaaccgg ggtctcacag atcaggggga gatttcgcag ggggggagtg
    10381 gagaggatcc gtgcaaaacc ctcttcaccg atccggccca gaccgatatg ttcatggcgg
    10441 tcgaggcccg agccgagatc gcctttcgaa tcgttcaggt gaatgagccg caggtgtttc
    10501 agaccgagtg tctcatcaaa ccggttcagg gtctcctcaa gtccttcacc tgaacgaagg
    10561 tcgtaccccg ccccgaatgc atggcaggtg tcaaagcaga agccaatccg ggaggatttt
    10621 ttgatacctt ccatgacagt agcgatatct tcgaaacggc tcccgattcc gttcttctcg
    10681 ccggctgtat tttcgaggag gagaagcacc ggattgtcta ccttgtccag ggcatcgttg
    10741 atcagggtgg agacacgccg ctgcccggca tccttccctt ccggcccata gtgtccaagg
    10801 tgggtcacca ggtaggggat ccccagcgtc ccgcaccgtg agagctcggc aaccagcgta
    10861 cttcctgatt tttccgcgaa atccggattc atggtagcca ggttggggag gtagggcatg
    10921 tggtcaacgg caggggctag tttgcttctc ccgagctcct ggcggaaaag gaccacctcg
    10981 gcaggttcga gatccctgaa catccagcct cttggattcc tcgaaaatat ctggaaggta
    11041 tcacaccctg tggctgatgc ccgtcccaca gcttttgaga tacccccggc gattgaaaca
    11101 tgtactccta tcgttaccat caaccggtct ccttttatca cctagggttg ggtgtgttga
    11161 tacttcgttt ttgggttcct cccttacctg ttcctgatct caaggggaaa caaacctcca
    11221 ttcaggcacg tcaggggaga aaggataaga atatgggatg atgatatcaa aaacaagccg
    11281 cacatcggac gatatgccgg cagttcttcg cctcaatatt ccggatggaa agaaaacggg
    11341 ggaggtgaga atcctatcga tataccccgg gaaagagagc agacggatac accaggatcc
    11401 agtcctgcca gggagggggg gcgatacccg cttttttcag caccggggca ccaacgcctc
    11461 gagttctgca aggacagccc cggcaaattc agaggtccct gcagttcctc ccaggtccct
    11521 ggtctttatt ccccgggcga ttgtcgccct gatagcctgc tcaacaagcc cgcaggatgc
    11581 gtgctctccg atatggtgga ggagcatggc tgcactcctg aatgctgcga cagggtttgc
    11641 aatatttctt ccggcaatgt ccggtgcact cccgtgcaca ggttcgaaaa gggagtgatc
    11701 ggtaccgata tttccgcttg gcagaagacc aagcccccca accagatatg cggtcacatc
    11761 tgacagaata tctccaaaca tgttggtagt gacgatcaca tcatagcgat ccggatgctg
    11821 aagcacatcg aggcaaagcg cgtcaatgaa gcgttccttt atctggattg cggctttccg
    11881 ggcttcctca aggcagatgt cccggaagaa tacatccgat ttgagaatgt tcgctttgtg
    11941 accgatcgtg atatcgtggc gtctcctggc aagggtgcag gcataccggg ctatccgttc
    12001 acttcctttt ctcgtgatgc agcggagcgt gcatgccgag tcatgctcta tcttttcaag
    12061 accggaatac agtccctcgg tattctccct gacaacgatg atgtcaaaac cctcacccca
    12121 gacaggcctg acattggcat agagatcaag ctctttccgg atccggacaa tgacgctccg
    12181 gtaatcacgc gagggagggg tggtaaccgc cccaaacagg atcgaatcgg cagtggcaag
    12241 cagcgcgata tcatcctccg ttattgcctc tccgccccgc tgccacctgt caaacccggc
    12301 ctccacctcg aaaaattcga tgtccggccg gatgatatga atcgcctcac gagccggcgg
    12361 tatgacttcg tgaccaatcc cgtctccttc gatgatggca accctcatct tcccgccctc
    12421 catctggcaa ggagatcact gactgcggag aagatttcag agggggacgc cccccaatgg
    12481 accactgccc cgaacttccc cccatgtgtc ccgattcctt ctctctcttt ccggcagcac
    12541 cgtgcgatga atggttcttc ggcgaccatt gaaagggggc agatatccgt cacctcgaac
    12601 ccttcaccat aacactcccg aagaaactgg gtggcagtca ggcacccggc caccttctcc
    12661 cctcccagga ggaggtcggc atcaagggtc cggtcaaacc ctgatgcccg gcagggatag
    12721 atgtcagcat ccacctgcga gatgtcccgg agggtgtgct cgaaggttac atcgagcccg
    12781 gagaacagac ccgcttcttc aagatccctg atacaggctg aaaggcttgg acggggaggg
    12841 atgacatcat acacggccac ggtctggaag agcgagaggt caggatcaca gacaaacgtg
    12901 gtatgttcat cgatcccggt gaagatggtg caccgcttac ccgaatctga ggcgaggcgc
    12961 acgagcagag cccggttatg cagctgtacc ttgtccgggt agagccaggt ctcaccaggg
    13021 ccagcgagaa cccgcgtctc tacgacctcc cgcatcaggc ctttccctgc cgggtccgtt
    13081 gtcacttcga ggatttccgg gccggaaggg gtatcatgga tcaggtagcg ggagaggaag
    13141 tacacctgat ctccacaggg gcgtgttgct gcaatcccca cctccttgca ctggcggggg
    13201 aagatcatcg cttctgcctc cagtgcgata cgaggcctcc ggctgcaagc atctggagca
    13261 tccgggggga gaggggccgg atatcccggg agatcccgga tacggtgatc agccccgccg
    13321 tgagatcgat ctcaaccata tccccgtcac tgcatggtat ctccgcctca gcaaggggaa
    13381 gcccgatatt gatggcattc ctgaagaaga tccgtgcaaa ggaaggggcg acgatggcgc
    13441 ggacccctgc ctccttcagg gctaccgggg cctgctcacg ggaggagccg catccgaagt
    13501 tctttccggc aacaattact gcctcgtgaa gcttttcagc aagatccgga tcgagatcct
    13561 caaagacatg acgtttccag atagaacgat ccttcgtccg gagataccgt ccggcaatga
    13621 tctggtctgt gtcgatatct gccggaagac agaccgcgtg cccttcgatc ttcataccac
    13681 tcctccgggg gcggtaatct cccctttaag ggctgatgct gcagcggttg ccactgaaga
    13741 gagatagatc tcccctccta cccccatcct gttcctgaaa ttccggttcg aggtcgagag
    13801 gcacacttct ccctctccga tcactcccat gtgtgcccct aggcagggac cgcaccctgg
    13861 cgggagcact gtgcagcctg cggtgatgag gtctgacaaa acgccggttt cggctgcctt
    13921 gcgaaggacg ttttttgagg cagggccgat gatggtccgt attttcacct ttttgccgcg
    13981 gactatcgag gcaaaacgga agagatcctc ataccgtccg tttgtacagg tcccgacaaa
    14041 aacctggtcg agggggatcc ctgccaactc atcgacgggc ctgacggtat ccacgcgggg
    14101 ggggacggca atcactggtt cgatcgcttc gagatcgaac gtatactcct gggagtatgc
    14161 acagtcaggg gggtcctgca ccgcggtttc gtggcggtat gacaggaggt acgcacgggt
    14221 cacctcatcg gcgtagaaca tcccggtttt tgcccctgtt tcaaccgcga ggttgcagat
    14281 cgtcatgcgg gagtccatag agagggagga tactccgtct ccaatgaatt cgagggcatg
    14341 gtaggtggcc ccatccatgc cgagccgggc aacgtacgtg agggcaagat cttttgcttc
    14401 ggctccgccc gacagagttc ctgaaaggaa aagccctatc gtatccggta tcctgaacca
    14461 ggtcatcccg gatgcgaaga tcgccgccat gtcagtagct cccacccctg tcgcaaatgt
    14521 tccaaaagct ccgagggtgc aggtatgtga atcggcgccg gcaatgatct caccggggag
    14581 cgcgattccc tctcccatca gctgatggca gatcccgcca ccgatatcag agaactggag
    14641 tcccgaagag cgggcaaagt gccgcagttc gtgctggaga tctgcggtgg tggaattgtt
    14701 ggccggcacg atatggtcaa agaggacata cgcccgggaa ggatggctca cttcagtgac
    14761 accaagtgcc cggaacgtct cataggtcaa tacacccgtg ccatcatgaa cataggcacg
    14821 gtccacctcg cggtcaacat actcccccgc gcctcctgcg aggatccgct ctgacagggt
    14881 tggcatcgct tactgctcct tccttacatg ccgtatcagg gcagtgagag tatcaggggt
    14941 aataccgcac ttactctccc cgagttcctt cacttcatcg agcactttgc agatctgttg
    15001 ttctgagagc gagtatccga gcgttgccac gacatgctcg agcgcctttt tgccggtgtg
    15061 cttcccgagg atgaaacgcc gttctccccc gaccatgtcc gggaggaagt actcataggt
    15121 ctggggatct tcgagtatcg cggcgatatg gatgccactt tcatgagaga aggcatgctc
    15181 cccgaccacc gctttcgtct tctgaaggga aatgcctgaa taggcctgga ccatcattga
    15241 gatatcgcac agatgggaga gatcataccg atccaccccc ccgttcatgc ggagcgccac
    15301 cagcacctcc tcaagcgcgg cattcccagc ccgctccccg atcccgttga ccgtggtatg
    15361 gagctgaaaa gcacctgcct ctgcagccgt gaccgtgttg gccgtggcac aacccatgtc
    15421 attgtgacag tgggcacaaa aaggccggtc aaccgtggag accagcgtgc tcattaccct
    15481 gaacatctgc atggggacca ggctgcctac ggtatcagca aaactgagaa ggtccgcacc
    15541 atgatccgca ccgccccggt atacttcgag gaggaactcc aggtcggtgc gtgaagcatc
    15601 ctcggcagca aaacgaacgg cgagcccgtg gtccctggca aaatcaacca tccggagtgc
    15661 ctcttcgagc acggcctccc tgggcttatg gtacttgtgc cttatatgcc gatcggaggt
    15721 ggcaatgaag atgctcacca tgtccacatt acagtcaact gccgcctcga catccccttt
    15781 acgcgcccgt gccaggcaac agatccgggc atcaagcccc atccctgcaa ccgtctttac
    15841 acactgtttc tcgtagggtg atacaacggg gaagccggct tcgattacct ccaccccgat
    15901 ctcatcgagt tttgaggcga ttgcctgttt ctcctcgcag gagaatgaaa caccaggggt
    15961 ctgctccccg tccctgagtg tcacatcaca gatctcaata ttccacggtt tcatggtgtc
    16021 tcccttccgg gcaggttccc tggacaacga gggttacggg ctcttccgga atggtaatca
    16081 attcttcgag ttgttctgtg gccgatgccc tgactgcgag gggatctgcc cactggatat
    16141 atttgcaggg ggacggagcc tcctgccccc cggtcattcc catgcaccgg ttctcgagca
    16201 cgatgcagag gagggcgagt cgcttttcat acacatcgat gaggctctgg agccccgaat
    16261 gcaggagtgc atagtcccct atcagggcaa catgcgtgct ccgggcagca accccgatgg
    16321 cactgccaag accataactg gcgcttgcga tgcgataggg aggattcagc gccaggatcg
    16381 aacatcctgc atcagcgatc gcctgtatcc ttttcctctt catgatgtca aacaccgggg
    16441 taaacgggca gttccgacaa aatgtccggt aaaagcccct gctctccgag gtttgtggcg
    16501 gagggattgc tgctgggggg agaaccgcca gatgttcggc aacaaaaggt ctcccgatct
    16561 catttatccg ggtgaatccc gccagtccgg gaggaggggg gtagaccgtc acaatccgcg
    16621 aatcgccact ggcagctccg acaccggcaa cagcgccgtt catcctgttc agccggctcc
    16681 tcgccgacca tgcaaacatc ccgggaagga gcgaatccgc ctgatccacc cttcctttca
    16741 tggtaaggcg gggatcagca atcctgcctt cgccgggcct ctgcggaaca gcagtcgcca
    16801 cggcctcgcc aaacaccagt tgcggagtaa cccggaggat ggcgatccgg gagaacgctt
    16861 ctgatgcgga gaatgcttct tcaacacttt cagaacagac ctccggatca ggctcgagca
    16921 cgggggtctg tgccagctcg ccaaaataac gggagtccat cgcgttcgtg gaacctgctg
    16981 ccatgggatc gtccccagca acaatcacca ctcccccgac cagtccctgg gttgtcgcct
    17041 ggacaaggag atcggcaccg ttgttcagtc ccacgttctt catgatcacg gccgccctct
    17101 tcccccggat cgaatcgccg atggcatact cgagcgcaac cttctccgca atcaccggtt
    17161 cggcctttgc catcgcggaa agttccgtga ccgggtatcc cgggaccgca tagcagcgat
    17221 aagcactccg ggaaagggca gaagccagca cctcaaatcc tttcattacg ctctgtccct
    17281 ccccggcacc aggtcacacc cggtacattc gatgcacatg gtgagagcac gacagggatc
    17341 aagccctgca gcggagagtg cctcggtatg gaatgcaaag aggcggagga gccgttcagc
    17401 agagggacgg cggaaccctg ataactcccc ggcgggattg agcggccgga ggacagggac
    17461 gaccccgagt tcacagaggt tcccgataca gcactctgcc tctttgtcgg tctccccaag
    17521 tccgatgata agattggaga atacatggtt cctcccaaac aatggtacgg aatcctcaag
    17581 ggcgtcccat acggaacggt acgaaagtcc ggggcacatg tccgtgaata actgctccgt
    17641 tgccgcctcc acattgaatt tcacctcagc caccccggca gcatgcagcc gctctgcagt
    17701 acctttgccg ggaaagattg agactccaat cgggagaccg aaccgctgca gtctcccggt
    17761 cacccggatg acatactcct cttcctcctc gatgctctcc agcaccccgc tggtaagggc
    17821 gatcgaatca ctcctgtccc gtacttgttc caccatcgct tcaatctctt cgggggtctt
    17881 tcttcctgcg ccaattgctg ggacctcgca gtatctgcat cggtacaaac acctcccgga
    17941 gaccgtgata tacgcctgcc tggggcagtg gagcgcaaca ggctcgagat tcccccttat
    18001 atcaagatgc caggctttca ggatggctct cccggatccg agatgaacga tctcgagcgg
    18061 gctgttctga tcaatagcaa gcctgacccg gcctttgccg caggagaaaa acaccgaacc
    18121 ccccgttccc gcacccggac cggcagtgga tgatgaacgg taccgcaatg caggttttcc
    18181 tgtgaggcgg accgtccctt ccgcaaggag ccttgccttc agctctggcc agtccataac
    18241 gagagtgcct cctcaaaacg ggtgtagaac ggaatcgcag cgaccgcacc aataattcct
    18301 ctcccttcca tgatcagttc aagatgccct ccctcgatct cgggcaggag atcgcgccgt
    18361 acctccccct tcttcaccat ccaggcaaag gggaggaggg gggagggatc aaatcctgtg
    18421 aatgcagcca tccctgtttc atccgagagt gtatacttcc ggagcagatt gtggaacctg
    18481 gcgaccagcc tgccgggatt ggcggaggca aattcgcaga cgatactgac acagtttttg
    18541 gtccggtacg gaacggggaa gagctgcacg atggtatgcg agaggtaccg tgccgtttca
    18601 tcttcaactg cccgggcaat attatgggca agcgtccagc tcgccccctg atccggggta
    18661 tcggtatcgt caactccgat caggacccgc tgcatccggg ggagccagat cgttgcacct
    18721 gccttcctgc cccctcccga aggatcggtc tggtgccgga tgacgccccc tgcatatgat
    18781 cggccggcag aagcgcccac tcctcctccg ccaagcccga tataggagat ggcaatctcg
    18841 ttatcgccga tttctgctcc acaaatcccg gcagggaagg acgacccctg gagatccaga
    18901 tacgcggttc ccggcctgag gaggtatcgg accgatgccc cgacactccg ggcagaccgg
    18961 accagcgggc tcccgacata gtggtgcctc gaccacatgg caccaccgat gcagtcaaaa
    19021 aattcgatga gttcgatcac tgttcctttt tcatcggcaa gagccacaat cccgggatac
    19081 cgtatttcat acggattgcc gggcagctcg tcctcttccc ctccctgagg cgatgtcccc
    19141 ggcctttctg aaatccggag ttctgggcat gcatcagctt cttttctcct ttttgccatg
    19201 agcaaaaaac gctcccgcga gatgacagtc tcacacccat attttcgttg ccgaaaatat
    19261 cgttaacaaa ataatcggca acaagatatt taatcctgat tgaaacgggt gggggagaaa
    19321 aattagagga ttttgttcag ttcaaatttg taaggaccaa gagatccgcc aaagttaccg
    19381 gcggtgatat attttacccc cggaaccatg caggcggcat ggatcccttt tgccattgct
    19441 tcacctacac atttctcatc gatcccatcg atcacaattt cgtacactcc ttttattcct
    19501 gccggcacct tgctgtccgg aaccttctcg cggatagtcg ggcagtactt ctcgttggtg
    19561 ctcgccggca tgaatttgta cttgtttgaa cctaccttgg aaccgcttgc caccacgccc
    19621 cctgggaaac tcgtgatgac cccgcagacc ttattgatag cctccactgc tgtctgggct
    19681 cccatgagtg ctgccatctg gttctctccc atgacaaaga aattgcctcc ggcaacccct
    19741 ttcacaatac caaattcttc ttcaccgata tattcgcctt ccatgatagg aatgacccag
    19801 catttcctgc caccgacttc tttctggtac tcatagccgt ccccgaagaa atggagtttg
    19861 accgcaatct tttctgccgc ctggggaaga ccatcaaaca ctgcggtggt aggagaggta
    19921 aggacacatt cggcgagacg ctcgaccacc tgctctttca gcttcttctt tgcagcgcag
    19981 atcaggatcg acaccccggg tcttccatca ggggtctggt ctgccctgag ttcactctcg
    20041 atcccggcct cgcaggggca tccgattgcc gatgtcgcaa aacccgtggc ttcggtcgct
    20101 gctttccggg cccattccgt gttcactgca gtgatgatga tccgggagat ccatgtgggg
    20161 aaggcttccg cgtaggtatc atcaattggt actccgttta tctccatgaa attcctcttt
    20221 ttttatacca aagatagtag gatagtgcta gaataaattt tctaaatacc tttccatgaa
    20281 aggttccccc ctgaactgca gaaaaaaaga ggtctttcat cagatgtgat cattacaatt
    20341 ggggaagaat tgaacaggga attactattt cgtacaggga agtgcgattt ttttccatga
    20401 aatgccccat gataccgatg ggtcacatgc ctccctcatg aaaatcgcgg atgggatttt
    20461 gatgccaaaa cgcctcctgc agtttcatcc cgttatgccc gaccgaagga gggatatcaa
    20521 ctctgtatat gaaaatgctc cggcaggtcc cccttgtatg cttctgttcc ctcaatgaaa
    20581 tcaggtaatt ttttccagga ggacgggtaa gggatatgta ttatatataa tttcctattt
    20641 gatgttagta cttaacgaag cgcagccaga ataagcggtg atttctcagc attatattaa
    20701 ttatacgggt taataaacaa tcttttattg acactggtcc tgttgaatcg gcctgaatgc
    20761 aggggggatt tagacaagtt tatgtgtata atttaaccat tcttttatag tcgatttttg
    20821 aatcgatcta aagctggtgc gtgatacaag catggcattt gcattgcacg ttaacatgga
    20881 gcgttgtacc ggctgcaata attgtgtggt cgcatgcccc gttgatgcgc ttgaactctt
    20941 caccatagat cctgcgacaa acgagaagat ctacaaggtg aagcacggga aagcgatggt
    21001 aatcgacttc aaatccgagc tctgtgccgg atgcggagtc tgtgtcgagg catgtccaca
    21061 tgatgtgatc cggctggcgg gacgcgggtc ggtaacatcg gaggccaaag ttggttaacg
    21121 ttctgaacat ggacaaactt gaggttattg aatgacactg tttccaaaat tctcaaagaa
    21181 gcgggatggc gtcaatgtca tccaggagca gaagcttctc cagcaggtga accacctgat
    21241 cctcaattcc gaggtctgca ccgggtgcgg tatctgtgtc gaagcctgcc ctgaagaggc
    21301 aatcgcactc ggccttgtcg gtgccacaag gcgaggcgcg gtctcatacg cagcgcccgt
    21361 agatatcgat gaggcgaaat gttcctactg cggagtctgc gtcgtcttgt gtccgtttaa
    21421 tgcactgaca ctgaagattg acggtgagga gcgtctcccc attgtggaaa aagagggctt
    21481 cccgacatat gacatggtgg cggatattga cgatgaaaag tgcgtcagat gtaccatctg
    21541 cgaggaggtg tgcccgagag atgccatcga tcgggatgtg accgaatttg aaggaacccg
    21601 ggctgatgga aaagaacgtc agctggccat caaatccaag accaccttca aggtagacga
    21661 tgagaagtgc accctctgcg gtatctgcgg ggtactctgc ccggcaatta ccgtcaagca
    21721 caagcctttc accgcagagt caggaaaggt cgaaggtgaa gtggtctggg acgagacgaa
    21781 gtgcgatgca tgcaaggtct gcgtggaggc atgccccgag gagtgcatca ccgtcgagcg
    21841 tgaggtcgag agtgataaac tggagggtca ggttgacatc gaccagaaga tgtgttgtac
    21901 ctgtacctgg tgttcgcgga gctgcccgac cgaggccatt accgtcgaga aactcttcga
    21961 aggggatatc gaattcttcc cggagaaatg ccctggtgga tgttccacct gtgtcgagat
    22021 ctgcccggca aatgccatat atctgccatc ttccgtgcca gcaagccagt taaagggcaa
    22081 agttgagccg accatcgcgg tcaacaagga cttctgtatc ctctgcggtg catgcgtgaa
    22141 tgcctgcccc ggtgaggaca tcatcaggct ccaccggacc ggcatccgcg tcaagggcaa
    22201 ggagaccgat cttttcaata aaatcaagga gaagttgtgc acccccagga cctccaaggt
    22261 caaagaggag accccgggaa gcgtacagct gaaaatgatg gagaaggcgt gaggggataa
    22321 gacatgccaa gaacgaaagg ataccctgaa gcactcgata agaaactcgc tgacacccgt
    22381 ctttatgaag atgagcgcca ccccgaattc acaaagaccg ttgaaaaact ctcaatgagc
    22441 atcccccaca tgtgctacca gtgcgggacg tgcacggctt catgcccttc agctccccgc
    22501 agctcatacc ggatccggaa gtttgtcagg agagcgctcc tcggccttga agacgaagcc
    22561 ctcaccgacc ctgatctctg gctctgcacc acctgctaca gctgcaccga ccgctgcccc
    22621 agggatatcg ctccgaccga tgtgatcatg gcgatgagga acctcgcatt caagagagac
    22681 atcatcccgg tgaacttctt aaagaccgtc caggccattt acaccaccgg gcatggtgtc
    22741 ccgaacaacg atgtgaaccg tgcagcacgg aagaagcttg ggatgaaccc tgaaccgcca
    22801 accacccatg cattcccgca gtatgtcaaa ggaatccaga agatcatcga ctactatgaa
    22861 ctcaagaaga acgccgacag aattgtcaaa gagagagagg gatgatgact gatgcgtgaa
    22921 tatgcatttt tcctcggttg tatcgcacca aaccggtacc ctggtgcaga atctgcatcc
    22981 tacaagaccg cagaaaaact tggtatcaag ctgcttgacc ttgaaggtgc cagttgctgc
    23041 ccggctccgg gcgcattcgg ttcaatatcc cttgaaatct ggtatgcaat ggccgcacgg
    23101 aacctttgcc ttgccgaaca gatgaagaag gatatcgccc tgatatgcaa tggttgttac
    23161 aagtcaatct gggaagtcaa ccacaagctc aagcagcaca aggaactgaa ggagaaggtg
    23221 aatgaggttc tcaagaccat cgacatggag ttcaagggga ccatcgatgt ctggcacctt
    23281 gcggaactct tttatgatcc tgaaattgtc ggtgtccaga agatacggga cagcgtgaaa
    23341 gtctccctgg aagggacaag gatcgccgtg cactatggat gccacctgat gaaaccaagg
    23401 aaggagaggc actttggcac caccgaacat cctacctggc tggaagagat ggttggagca
    23461 ctcggcgcag aacccgtcga ataccgaaac aagatgctct gctgcggcgc aggtggcggg
    23521 gtccgaggat acgatctggt ccactcactg gatatcacca atgagaaaat gataaacctc
    23581 aaggaagccc gcgtggatgc cctcaccgaa gtatgtccgt tctgccagct ccagtttgac
    23641 aggggacaga tcgagattga ggagaagttt ggtgtgaaat acaacctgcc ggtactccac
    23701 tattgtgagc tgctgggact ggcacaggga atgagcccgc aggagcttgc acttgacctc
    23761 catggtatca gctgcgaacc attcctgcag aaggtcctgt aaaggaggga gaaaagatgg
    23821 cagaaaagaa gacaaagaaa gagactgctt ccaagaaaaa ggaaacaccc aaggctgccg
    23881 taccagcaaa gactgagaag gcacccgccg taaaagctcc cgcgactgag cagaaaaagg
    23941 cccgcgtcgg tgtattcatc tgtcactgtg gtaccaatat tgccggatca gttgacatcc
    24001 ccgatgtaca ggagtatgca aagagcctgc caaacgttgc atttgcagac aactatcaat
    24061 acatgtgctc caccccgggt cagtcaaaga ttattgctgc gataaaagag aatgacctga
    24121 ccggcattgt ggtggcagcc tgttcaccac gtctgcatga accaacgttc aggaccgcca
    24181 ccaaggaagg agggctgaac ccgttccggt tcgagatggc caatatccgc gaacagggct
    24241 cgtgggtgca catgcatgac cgtgaaggtg ccaccgataa agccaaggat gctatccgga
    24301 tcgccgttgc gaaagcgtcg ctcctgcagg acctcatccc gaagaccgtt cccgttgagc
    24361 atgcagccat ggtggttggt gcagggattg caggcatgca ggccgcactc gacctcgcca
    24421 atgccgggat aaagacctac cttatcgaga
//
LOCUS       DAOQYR010000024        24366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_2404_length_24366_cov_9.028464, whole genome shotgun sequence.
ACCESSION   DAOQYR010000024 DAOQYR010000000
VERSION     DAOQYR010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 24366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24366
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2404_length_24366_cov_9.028464"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PKM87_04625"
     CDS             complement(<1..573)
                     /locus_tag="PKM87_04625"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNQ29386.1"
                     /translation="MNITALRPRFVRYLEPVAAFFCRIGATPNQVSLLALFFGLLCAY
                     LFFRQMFLAGSLALIISAVLDLVDGAVARQTSRQTQFGAVFDWIVDKYVDALVLLGIG
                     LSGIAIITRFTPLPPLADFAVVAFAITGSLMNTFIKPVVYAEIGYQERVDGKIEDPLE
                     GVGFFGRPETFLVLLAGGLTGFIWLSVLVIA"
     gene            743..1459
                     /locus_tag="PKM87_04630"
     CDS             743..1459
                     /locus_tag="PKM87_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNQ29387.1"
                     /translation="MPEPKRRPHVLMMSEITVDGKLTLKKGASSKILMKYMDPATEIL
                     LHKTRAACDAIMVGSNTIRIDNSFLTVRHVEGKSPLRVIPSSMADIPPDANVLGPGAP
                     TVVAVAARAPQERVEAIRRRGVHVLVSGEERVQLPLLMEQLFDQFSVRTMMIEGGPTL
                     NWHMLHHRLVDEIRLIHLPFIVGGADTPSLVGGMHIEDESEMICLTLKNHYLCGTNLV
                     TEYQVRYNPGGAGDAGDSGR"
     gene            1437..2210
                     /gene="sppA"
                     /locus_tag="PKM87_04635"
     CDS             1437..2210
                     /gene="sppA"
                     /locus_tag="PKM87_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNQ29388.1"
                     /translation="MQETPAAEGTGRRRNQLLVVLVLAGAVICAAASFILFTTYAKDL
                     GISVVRVEGMIVTGNSRGDGYVGSEYVGRMIREAADDPIVDAIVIRVNSPGGTPAAAQ
                     EIAADIRYARNKKPVVVSMGDIATSAAYYVSAYADRIYANPDTLTGGIGTSWSFLDIS
                     GWLEEKNISVVVFKSGRMKDMGSDHRPLTPEEREYAQMVVNQSNERLLTGIIAERGIN
                     QSDIEDARVIRGEQALLLGLVDEIGNLNDAIAGARRLAG"
     gene            complement(2252..3520)
                     /gene="truD"
                     /locus_tag="PKM87_04640"
     CDS             complement(2252..3520)
                     /gene="truD"
                     /locus_tag="PKM87_04640"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HNQ29389.1"
                     /translation="MRETPYPLEQSLGMRWYASNTPGIGGRLRTTAEDFVVEETSSGP
                     GDEGPYLICRLTKKDWELQRAVREIAHALSISHRRIGWTGTKDKHAVTTQLISIYGVS
                     EDDLGRIRLKDLEVVPVGRSREPLLLGAHDSNHFTITIRDTTSPDLGSEVAEVAAACG
                     RGIPNYFGIQRFGVIRPITHLVGEHILKGDFDLAVDCYVGMACPGEPPAVREAREAYA
                     EEKDTLAALRSLPVPLTYERAMLHHLVSHPGDFRGALLSLPPKLLSMFVSAFQSYLFN
                     MALSARMERDPDFSLPAPGDRLIFSNGREDTVTVANRDAAMMQVARGRCKVAIMMPGS
                     RAVRETGEDDRAVAALLSMHDIVPDSFSRASSFVKARYDGALRPVPLTAEVMSTVTGP
                     DVTLSFSLAPGQYATTVCREFMKADPRAMV"
     gene            complement(3567..3929)
                     /gene="pth2"
                     /locus_tag="PKM87_04645"
     CDS             complement(3567..3929)
                     /gene="pth2"
                     /locus_tag="PKM87_04645"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase Pth2"
                     /protein_id="HNQ29390.1"
                     /translation="MEKEPRFEWKQCLVVRSDIRMSCGKTCAQVAHAAVIAYDRTDAS
                     TRKKWLQEGQKKVVLKVPSERALLELKTLAEYAGISTGLVQDAGLTEIPPGTVTALGL
                     GPAKSEDLDRITSSLSLF"
     gene            4273..5439
                     /locus_tag="PKM87_04650"
     CDS             4273..5439
                     /locus_tag="PKM87_04650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ29391.1"
                     /translation="MTRQYDVVVVGGGPAGSTCGRLCAEQGLSVCLVEEHGAAGIPVQ
                     CAGLLSMNAFRECEVSQSSILATVRGAKVVSSLGPVLEFDARMPKAHVVDRAVLDREM
                     LARAAGAGAEVRMKTSFADRKGQRIVTRGVNGKEEILFRILVAADGARSRVARVMGMR
                     RPEVFLAGLQADILHGMDGSRVEIHPDAAPDFFGWAIPIGAGRARVGLAGRTGVRERF
                     RSFLSLISQDSHQAQVHLVSGTIPLGTMARTFGDRVLFVGDAAGMAKPTSGGGVYTGV
                     RAARHAARVAGEAVALEDFGDGMLSRYERAWKEDFGRELEQGMQVFRLRQQISPGDMA
                     KIVSALSDPAIVEDIVELGDMDRPGRLARRLLTRPSLYPLLGILIRSGVRLIYE"
     gene            5501..6139
                     /gene="cbiM"
                     /locus_tag="PKM87_04655"
     CDS             5501..6139
                     /gene="cbiM"
                     /locus_tag="PKM87_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HNQ29392.1"
                     /translation="MHIPDAFIPLTQAAVYWVIALIFIALSLRWARNEMKEEKVPLVA
                     VLAAGIFAIQAVNVPIPWGTSGHMMGAALAAIVLGSPFAAIFVLTLVLIVQGVLFGDG
                     GITVMGANIINMGVIGGFVGFYSYKGLNSAIRNPYPAAFIAGWLSLFVAALVCAVEMA
                     IAGTFPLEIGLISMGLYHAVIGVIEGGITAVALYLIYTARPDLISDNAGVAA"
     gene            6139..6486
                     /locus_tag="PKM87_04660"
     CDS             6139..6486
                     /locus_tag="PKM87_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HNQ29393.1"
                     /translation="MGRENLIYFGVIIALVIAVAAPFVASSNPDGLESAFFGVFGAKE
                     VQGSDLDEEAAGAAEEQVQEVTGNTFSFASPFPDYSIEGMEKAGEALVIVIGTLLVLA
                     IAFGLGRVLSRSE"
     gene            6538..7371
                     /locus_tag="PKM87_04665"
     CDS             6538..7371
                     /locus_tag="PKM87_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ29394.1"
                     /translation="MHLIETRDLSYTYPGNVRGLDHVNFIAPRNARIAVIGANGAGKS
                     TLFKHFNGIFRPTSGSVLVHGEPITRENIKEVRKFVGVVFQNPDDQIISPTVEQDVAF
                     GPTNLGLDEETVNHRVEEALRVVGIEHLKGRVPHHLSGGEKKRVAIAGIIAMEPQVLV
                     LDEPTAGLDPQGVRDLVGFINSLSRTYGMTVIFSTHDVSLVPEVADIIYVMHRGRVVA
                     EGDCREIFGRSDLLSSVRLDVPVLPKLISSLRDQGVEVSMAYTFNEAERNFLAAYKRN
                     G"
     gene            7368..8195
                     /gene="cbiQ"
                     /locus_tag="PKM87_04670"
     CDS             7368..8195
                     /gene="cbiQ"
                     /locus_tag="PKM87_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HNQ29395.1"
                     /translation="MIEDLFFIEKQTYKKSLIHDLDARVKIAVCFAVIVAMVAVPYSP
                     LVFPVAVTFAVFFAALWALSRLSPLAYVKRLVMILPFGLFIILVQVFVQNPYYTVFTP
                     IADLPMGLHIYRESIEFATILLLKFLVCVSFIILLSSTTRVQDLLEGAGRLGLPAEFT
                     LTLGMMIRYIFVFGYMYRKINESLATRYFVPFDSHLPYRYRLRTIAYTIGTLFIRSYE
                     QGERTYTAMLCRGYGRESHLYIRKKPLFRKDHAFLAAGLLVAVCTPIFFWVSWIGLI"
     gene            8234..8542
                     /locus_tag="PKM87_04675"
     CDS             8234..8542
                     /locus_tag="PKM87_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29396.1"
                     /translation="MASTFGYGFITNLMHICKHFSLKPEEAFYGAADHLDGFVIPDQF
                     KGTEIEEIADRLRKRIVWHQPGTLDKEEAAEVVRLINRLIIAIDKALGIKDPDLGEFH
                     "
     gene            8645..10699
                     /locus_tag="PKM87_04680"
     CDS             8645..10699
                     /locus_tag="PKM87_04680"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HNQ29397.1"
                     /translation="MAETYDASHITILEGLQPVRERPAMYIGSTDSRGLHHLVYEVVD
                     NSIDEALAGYCDRIWVSINKNGSVTIEDNGRGIPVDRMEKNGKCALEVVLTVLHAGGK
                     FDKDTYQVSGGLHGVGVSVVNALSSWLDARVYRNGNIYEMRFSQGKTISQCNEREESL
                     EELRARLKHWYLEPEIPEGSAGQAGSIPAKELADDSKLDRNQFLQKFGPKLSGTRITF
                     NPDSSIFETVHFDYDVLAHRLRELAFLNAGLIIFITDERNGDHARYQYLGGLSEFVRY
                     LNEGAECLNPVPIFISKKDEENRLEFDVAIQYTTGYDEKIFSYVNSVNTREGGTHLEG
                     FRSAITRAINTMAKRNGLIKESSSLSLRGEDVREGMTAVVSIKMANPQFEGQTKMRLG
                     NSNVKGIADSLVYSALSEYFDENPKVLQIIVEKALSAAKAREAARNARELARRKGALE
                     SGGLPGKLADCSERNPEKSEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNVEKAG
                     EHQLLKNAEIQTLVSAIGTGIGENFDISRARYHKIVIMTDADVDGSHIRTLLLTFFHK
                     SMPKLIDAGYIYIAQPPLFSVSKGKVEKYAYREEDMKRLLADMGEKGISVKRYKGLGE
                     MNAQQLWDTTMNPENRIFKKVMIEDAMEANEIFKTLMGKDVEARKDFIKRHAREVKNL
                     DV"
     gene            10689..13190
                     /gene="gyrA"
                     /locus_tag="PKM87_04685"
     CDS             10689..13190
                     /gene="gyrA"
                     /locus_tag="PKM87_04685"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNQ29398.1"
                     /translation="MTSDPDLPRDDAVTERVEEVSIEKEMKTSFIDYAMSVIIQRAIP
                     DVRDGLKPVHRRSLFAMWEMGNTSDKPTKKSARVVGDVMGKYHPHGDASIYDTIVKMA
                     QPFSYRHTLVEGQGNFGSVDGDAAAAQRYTEVRLRPYAEALLEDIEKETVPLVPNYDE
                     SLTEPVVLPAKIPNLLVNGTDGIAVGMATKMPPHNLREVCTAVVAYLDNPAITVEELM
                     TSLPAPDFPTGGILMGVEGVRNIFTSGQGRLVVRGVATIEEPKSGSRNPRIIVSELPY
                     QVNKAQWITAIADMVKNKRIEGISDIRDESDKEGIRVVFDLKKDAIPSVVLNNLYKNT
                     QLESSFWAINYAIVENQPRLLNLPELLDHFVRHRIEIITKRSEFDLKKALEKVHILNG
                     LLIALARIDDVIAAIRASETVEDARNALITRFGLDEPQANAILQMQLRRLAALEQKKI
                     IDEKAGLEAEIARLQHILSSEETIKAEIRRETMEVSEKFGDARRTQISLNFGDISNED
                     LIEDKTVLVSITTANYIKRTDIDVYRKQRRGGHGITGMTTKEDDVVDSIFTASLKDYL
                     LCFTNTGRVYWLKVYQIPETSRTAKGKPIVNLLNLKDEVVTTVIPIREFRENQFLLFA
                     TKLGQIIKIPLKDFSFPRSVGTNAIKLRGSDQLVEVILTDGNKEIILTSRFGQSLRFH
                     EETVRTVGRTAQGVRGMKMKGGDTVVAVTLVEKDHLLTISDVGFGKRTEFDEFRGHGR
                     GTMGVRNIQLERDAVVIASCAVMDTDEIVVMTASGIVIRIHVNEIRVIGRGTKGVKIM
                     RLDENDRIIGIAIVPQDDESGQNGTENGTFTPDDE"
     gene            13401..13814
                     /locus_tag="PKM87_04690"
     CDS             13401..13814
                     /locus_tag="PKM87_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNQ29399.1"
                     /translation="MYQTTLHLSTCGEGDIVDITPRIREEVKTSRVAEGLVNVFVTGS
                     TAAVTTIEYEQGVLSDLQRALSVVAPDNIPYAHDSRWGDGNGRSHVRAAIVGPSLTIP
                     VAGGEPLLGTWQQVVLLELDVRKERARTVVITVIP"
     gene            complement(13874..14137)
                     /locus_tag="PKM87_04695"
     CDS             complement(13874..14137)
                     /locus_tag="PKM87_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0147 family protein"
                     /protein_id="HNQ29400.1"
                     /translation="MTNPDKTIESCIQMLQHIMDDSTIPRNIRRVADETRQLLMNNSK
                     NSGLRAAEAISKIDEISNDPNMPVHARTRIWELVSQLETIPLD"
     gene            14322..14702
                     /locus_tag="PKM87_04700"
     CDS             14322..14702
                     /locus_tag="PKM87_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sjogren's syndrome/scleroderma autoantigen 1
                     family protein"
                     /protein_id="HNQ29401.1"
                     /translation="MKREDEIMAEHLLRGGKMLSASCPVCGCPLFEVKGKTLCVVCAE
                     GKAEKEEENQAVIPGQATAAQAPPPAASSPRPIASSRLADALEETLVALCARIREEKD
                     PRCVAALMEAVVTGIEGLGALRPR"
     gene            complement(14705..16939)
                     /locus_tag="PKM87_04705"
     CDS             complement(14705..16939)
                     /locus_tag="PKM87_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNQ29402.1"
                     /translation="MNTITHPFIAPGRLESRTYQLSIAMKALEGHTMVVLPTGLGKTA
                     IALLVAASRLYNQGGRVVMMAPTKPLVEQHLRFFRQFLSLPSQEDDTLAMFTGETPSG
                     ERAAAWKKARMVFATPQVIKNDVLAGSYDTRDVSLMIFDECHRAVGNYAYVFLAQRYL
                     ETAASPLVLAMTASPGGQVEKVEDVCRNLGIVQVESRTEHDRDVAPYIHEREIEVVEV
                     DLPPELKKAASDLDALVDSRLTQLKHLGFRVPPRQQLSMKSLQALNAQVQEQIQARES
                     SGYAAASIYAELMKLRHALSLAESQGSRVLAGYITRLFSEGSGGSGPKATARLARDPV
                     FIRLLERCMSWNEELHPKMEILSRVVKDQLEASPESRIIVFATFRDMVQQIVDHLNSE
                     GIACQRFVGQATKDSEKGLSQKRQIEALQKFRAGDFRVLVATSVGEEGLDVPSTDLVV
                     FYEAVPSEIRSIQRKGRTGRSGKGRIVVLVTKGTSDEVFRYVSQNRERSMLSGIKHIS
                     PSSQGLPGKRPQPPVQSSIGEFAGRGPEVRVDDRETSSKVVEILSNMGVSLVLGRLPS
                     GDYAIGDRILIERKTARDFVDTLVERDLFGQLKALAESAPRPVLIIEGGDLYTARDIH
                     PNAVRGSLAAIAVDLGISILFTKDEEDTAQMILVLARREEGGERERTLPMKRHGHSLR
                     EQQELVIASFPDIGLKNARLLLDHFGTVRGVVNATEEELLTIRGIGEQRSKKIHELAR
                     RPYR"
     gene            complement(16936..18660)
                     /gene="glyS"
                     /locus_tag="PKM87_04710"
     CDS             complement(16936..18660)
                     /gene="glyS"
                     /locus_tag="PKM87_04710"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNQ29403.1"
                     /translation="MSDIYDKIMDLARRRGFIWPASECYGSVAGFIDYGPLGSMMKRR
                     IEDTWREFYVVREGYYEIECPTIGQEDIFIASGHVKGFSDKMCQCPHCREYLRADHIA
                     EGQGIPGASTMDTGELSRVLSTLPCPVCGEVLGEVEVFFFNLMFQTSIGPGSQRKGYL
                     RPETAQGMFTDFQRLLRFYRDTLPFGAIQIGKSYRNEISPRQGMIRLREFTQAEAEIF
                     VHPDEKDHPFFSRYAGYRMPLLGIRQQQEETPALETTMDEAVREGLIANQYVAYYLAL
                     THHLLVTVGVDPEKLRFRQHLPDERAHYAEDCWDAEIFSERFGWVETVGIADRTDYDL
                     RAHEKQSGQRFAVFIPYETPRKERRVRVVANMGALGPRFRGKAKAVADALADVVPGPD
                     GVTLTIDGEEVFISPDLYQVREEEVEIPGEEITPHVIEPSYGIDRMIYAVLEHRYAEE
                     TVNDEERRVLRLPPRIAPIQAAVFPLMNRDGLPEIARDIVTSLKARGIMAEYDDSGAI
                     GRRYRRQDEVGTPFAITVDYETPETGTVTLRDRDSMEQVRVPAETLPDLIRSLVEGSA
                     TFASLSPA"
     gene            18877..19485
                     /locus_tag="PKM87_04715"
     CDS             18877..19485
                     /locus_tag="PKM87_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver protein"
                     /protein_id="HNQ29404.1"
                     /translation="MQDDKRKKELLQLFTNISGKTKIVEPMKKIHGTLRDRDAVEREI
                     ALVMREILEQGFFKTKLKPIQLARLVSAYYAGKNDTEIARELGDEKLSKTVARARVRL
                     KLFRELDFKMPFDQDRMEDLLISGKTMKEVSEDLGVSPSTLREYRHVIEQRDDVTIDP
                     FLERIKDVMEDRDLSEQMTRSATKDSLGEAIDITEAEMIDVT"
     gene            19556..20209
                     /locus_tag="PKM87_04720"
     CDS             19556..20209
                     /locus_tag="PKM87_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNQ29405.1"
                     /translation="MKLTWFGHSCVLLEGSRKVLIDPFIPEGSIDVEPDVVAVTHGHA
                     DHMGDAASYGVPVVAINEVAKYLRTKGVEAEAMNIGGTIEVKGVRFTMTPALHSAWIE
                     EAGIGCNGGCAAGFVIRMDGKAIYHAGDTGLFSDMSLIGELYHPDVALLPIGGRFTMG
                     PEEAMVAARMIGSPLVIPIHYNTWPAISQDAEGFKRTLERTTDCRVRVLAPGGSITL"
     gene            20670..22937
                     /locus_tag="PKM87_04725"
     CDS             20670..22937
                     /locus_tag="PKM87_04725"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNQ29406.1"
                     /translation="MHVITGEYQIRRSIPRISSLVLVLAICTFCNCMIAVADATNGEG
                     TEQAIGIDMSDAAHSSKAEPNYEVVFPADRVNIMTITITPGDWEKMRENMTEMYGEFG
                     EGISGGGPGQAGEPGGNPGERQKIVGMFSEVDPVYVPADVSFEGVGWQNVGIRFKGFN
                     SLQGAWREGSYKISLKLNFDKFEDEFPEIKNQRFYGFDELNLQGNFNDDSLLREMVVP
                     SIFRESGVPAPYTAFYRLYVDYGDGPKYFGVYTMVEAVEDTLIETQFADDSGNLYKPE
                     GNGATFAAGTFDRDSFEKKTNEDEADWSDVETLYAVLHSDMRLSNPVKWRAELEEILD
                     VDVFLRWLATNTVIQNWDTYGGNCRNFYLYTDPADGRITWIPWDNNYALDNGMGGGGQ
                     VPGGTNPGDPGGRNWTPGGMNPGEMEGRSRTPGEINPGDLGERNWTPGDMNETPGVGM
                     GSTLSLGLDEVGENWPLIRYIADDPVYYTRYQEYLGIVVTTSFEPSKMEETYEYYHAL
                     IEPYVTGQYGEQAGYTHLDNPRDFDTALDDLIEHVKNRYDAVMEFLTLAPVAKPTPTP
                     TMAGLKDLTPPTVRITTPAQSAQTAPGEALQIAWSATDNTGVEGVAIFYTLDRGKTWI
                     QIADDLPEEGSFTWTVPKDAGSTLIVKVSAADAAGNVGSASRVCTCTSATTTAVLKGG
                     NLIPSPIKPVSSFSDSWNIKSLQNGNTNSEFIANSPESARFLSPVTKPVTLSLQKAAT
                     FSSQSGRDSLSSGRG"
     gene            22949..23776
                     /locus_tag="PKM87_04730"
     CDS             22949..23776
                     /locus_tag="PKM87_04730"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HNQ29407.1"
                     /translation="MNEETAGMQYGPTHCAGPGSRMLTATRDFGSISLKGLEEAKAGL
                     LIREESKHLMTLEQCLAFLAGLPDSYCVLEVNNSRITRYETLYYDTPSFLTFLQHHNG
                     KGNRYKLRIRRYDSSKETYLEVKKKTSKGTTEKSRVRTWWTTAGFTPEQEEFLEAAFP
                     YDYREFFPVVKTVYDRLTLVSTKSPERITLDSNISFLSGQEQKTYPGLVIVEIKHEKG
                     IRASPALSALHTLGIRKRGFSKYCIGVSLLYDRVKHNRFKQNLLLLSKLDSVRCQAC"
     gene            23770..24360
                     /locus_tag="PKM87_04735"
     CDS             23770..24360
                     /locus_tag="PKM87_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HNQ29408.1"
                     /translation="MLIDATPLFIAGFVLNFLAAFIIVRFVYYPRGRCNNYVFTFLAF
                     NTVIYFIMGMFTSVEISIGAGFGLFALFSILRYRTETVPIREMTYLFVMAALPILNSI
                     FWQSAQYEKLLIVDILVIAVIWFLDREWGFNREQFQMNVMYEKIDLVKRERRREMLAD
                     LCERTGLAVTDAEVQSINFLRDVAELKVTYTNRRHD"
BASE COUNT         5343 a         6830 c         7274 g         4919 t
ORIGIN      
        1 ggcaatcacc aggaccgaga gccagatgaa cccggtcagc ccgcccgcaa ggaggacgag
       61 aaacgtctcc ggcctgccga agaaccccac tccttccagg gggtcctcga tcttcccgtc
      121 cacccgctcc tggtagccga tctcggcgta gaccaccggc ttgatgaacg tgttcatcag
      181 cgaacctgtg atcgcgaagg caaccaccgc aaagtctgcc agtggaggga ggggagtgaa
      241 ccttgtgata atggcgatgc cggagagacc aataccgagc agcaccaggg catcgacata
      301 cttgtctacg atccagtcaa agactgcccc gaactgcgtc tgccttgagg tctgccgggc
      361 cactgcaccg tccaccaggt cgaggacggc ggagatgatg agtgcaaggc tcccggccaa
      421 aaacatctgc ctgaagaaga ggtaggcgca caggagcccg aaaaagagtg caagaaggga
      481 cacctggttt ggggttgcgc cgatccggca gaagaatgct gccaccggct cgaggtaacg
      541 cacgaacctt ggccggagag cagtaatatt catgcctaag taccatgccg ggtggaccaa
      601 taatgattcc catcccgcat cgtatcgcag ggcgcatatc ctctgttttg gaggaatcgc
      661 gaatcaattc ttctcgtgcg gagccaggga acttctatat gagagcaaca agatatgagt
      721 ctatcaggag actgaatctg ccttgccgga accgaaacga cgaccgcatg tcttgatgat
      781 gtcagagatc accgtggacg ggaaactgac cctgaaaaag ggggcatcga gcaagatcct
      841 gatgaagtac atggaccctg cgaccgagat cctcctccat aaaaccaggg cggcctgcga
      901 tgccatcatg gtgggctcaa ataccatccg aatcgacaat tcgttcctca cggtcaggca
      961 tgtggaagga aaaagccccc tgcgggtcat cccgagcagc atggccgata tccccccgga
     1021 tgcgaacgtc ctcgggccgg gtgccccaac ggtggtggcc gtggccgccc gggccccgca
     1081 agagagggtg gaggccatcc gaaggcgggg agtccacgtg ctggtctcgg gcgaggagcg
     1141 ggtgcaactc cccctcctca tggagcagct ctttgatcag ttctctgtcc gcaccatgat
     1201 gattgagggt ggtcctaccc tcaactggca catgctgcac caccggctcg tggatgagat
     1261 ccggctcatc catctcccct tcatcgtcgg gggggcagac accccctcgc ttgtcggggg
     1321 aatgcacatc gaggacgaga gcgagatgat ctgcctgacc ttaaagaacc attatctctg
     1381 cgggaccaac ctggtgaccg agtaccaggt ccgttacaac ccgggaggtg ccggggatgc
     1441 aggagactcc ggccgctgaa gggacgggca ggcgaaggaa ccagttactc gttgtactcg
     1501 tccttgccgg cgcggtcatc tgcgcggctg catcatttat cctgttcacc acctatgcaa
     1561 aggaccttgg catctccgtg gtgagggtgg agggcatgat cgtgacaggg aacagccgcg
     1621 gggacgggta cgtgggttcc gagtacgtgg gccgcatgat cagggaggcc gcagacgacc
     1681 cgatcgttga tgcaatcgtg atcagggtca acagcccggg cggaacccct gccgcggcac
     1741 aggagatcgc ggccgacatc cgctatgccc ggaataagaa accagtcgtg gtctccatgg
     1801 gcgatattgc cacctcggcc gcatactacg tgagcgcgta tgcggaccgg atttacgcaa
     1861 atcccgacac cctcaccggg gggatcggga catcgtggtc gtttctcgac atatcggggt
     1921 ggcttgaaga gaagaacatc agcgtcgtcg tcttcaagtc cggccgcatg aaggacatgg
     1981 gctcggatca ccgtcccctc actcccgaag agagggagta tgcgcagatg gtggtgaacc
     2041 agagcaacga gaggctcctc acgggtatca ttgcagagag ggggataaac caaagcgata
     2101 tcgaggatgc aagagtgata cggggcgaac aggccctcct tctcggactg gtggacgaga
     2161 tcggcaacct caacgacgcg atcgcggggg ccaggcggct tgcggggtaa tatcctggga
     2221 taccagtaac gcccccacca ctaccacagc attagaccat tgccctggga tcggctttca
     2281 tgaactccct gcacaccgtg gtggcgtact gccccggcgc aagagagaaa gagagggtca
     2341 catccggacc cgtgacggtg ctcatgacct ccgccgtaag cgggaccggc cggagggcac
     2401 cgtcgtacct ggctttcacg aacgatgagg cacgggagaa cgagtccggg acgatatcat
     2461 gcatcgagag gagtgcggca acggcccggt cgtcctcccc tgtctcccgc acagccctgc
     2521 tgccgggcat catgatggcc accttgcagc gcccccgtgc aacctgcatc atggcggcat
     2581 cccggttcgc gacggtgacg gtatcttccc tcccgtttga aaagatcagc cggtcaccgg
     2641 gggcagggag ggagaaatca gggtcccgct ccatcctggc agagagcgcc atgttgaaga
     2701 ggtaggactg gaaggcgctc acgaacatcg agaggagctt tggggggagg gagaggaggg
     2761 caccccggaa atcaccgggg tgcgagacga ggtgatggag cattgccctt tcgtaggtga
     2821 gggggacggg gagggaccgg agcgctgcaa gggtgtcttt ttcttctgca tatgcttccc
     2881 gtgcttcccg caccgccgga ggctcgcccg gacaggccat gcctacgtag cagtcaaccg
     2941 ccagatcaaa atcacctttc aggatgtgtt ctcccacgag gtgggtgatg ggccggatca
     3001 cgccgaagcg ctggatcccg aagtagtttg gaatgccccg gccacatgcg gcagccacct
     3061 ccgcgacttc ggaacccagg tcgggggaag tggtatcgcg gatggtaatg gtaaagtggt
     3121 tggagtcgtg ggcgccgagg aggagcggtt cacgggacct cccgaccggc acgacctcga
     3181 ggtccttcaa ccggatccgg ccgaggtcat cctcgctgac cccgtaaatc gagatgagct
     3241 gggtggtcac cgcgtgcttg tccttggtcc cggtccaccc gatccgccgg tgggatatgg
     3301 agagggcatg tgcgatctcc cggaccgccc gctggagttc ccagtctttc ttggtgagcc
     3361 tgcagatgag gtatggcccc tcatcgccgg gcccagaaga ggtctcctca acaacaaaat
     3421 cctctgccgt ggtgcgaagc cttccgccaa taccaggagt gttgctggca taccagcgca
     3481 taccgaggga ctgttccagg gggtaggggg tctctctcat gtgggattct cgcatctata
     3541 cgggtggacc cgggcgcgtt ttgccatcag aacagggaaa gcgaactggt gattcggtcc
     3601 aggtcctcgc tctttgccgg gccaaggccg agtgcggtca cggttcccgg cgggatctcg
     3661 gtgagccccg cgtcctggac aaggccggtg gagatgccgg catactcggc cagggtcttt
     3721 aattccagga gcgcccgctc tgaaggcacc ttgagcacca ctttcttctg accctcctgc
     3781 agccatttct ttcgtgtcga ggcgtcggtc cggtcgtatg cgatgactgc agcatgggca
     3841 acctgcgcac aggtcttccc gcagctcatc ctgatatcgc ttcgcaccac caggcattgt
     3901 ttccattcaa accggggctc tttctccatc tgtggtgaaa tattcgcggg cagaggagaa
     3961 aataggtgtg aaggggggaa acgccgcaag ggctcctcgc ctcgaccccg gggcgatatg
     4021 tctgtcgcca ggattcaccg tttttttcag cggggagggg gagaatcccc ccaccccccc
     4081 ctcctattcg cgatagactg catgtaaggt acagagggca gatatgatct cctttggatt
     4141 tcccagcact ctccactgca gtaagatgaa cgctatcagg aacgccggga cgtggtgcag
     4201 gaaatgaagt tttcagaaga cgtggacaac cttatacctt caagcagctc tggcaccagt
     4261 tcattatcag gaatgaccag gcagtatgac gtcgtggtgg ttgggggcgg gccggcaggg
     4321 agcacctgcg gccggctctg cgcggagcag gggctctccg tctgcctggt cgaggagcac
     4381 ggcgcggcgg gaatccccgt ccagtgtgcc gggctcctct cgatgaatgc gttcagggaa
     4441 tgcgaggtct cgcagtcgag catcctcgcg acggtccggg gtgcgaaggt ggtctcgagc
     4501 ctcggcccgg tgctcgaatt tgacgcacga atgccaaagg cccatgtcgt agaccgtgcc
     4561 gtgctcgacc gcgagatgct tgcccgggcg gcgggcgccg gggccgaggt caggatgaag
     4621 acctcgtttg cagaccggaa aggccaacgc atcgtcacac gcggcgtgaa tgggaaggag
     4681 gagatattgt tcaggatact cgttgcagct gacggagcac ggagccgggt ggcgagggtg
     4741 atgggaatgc gccggcccga ggtcttcctt gccggtctgc aggcggacat cctgcacggg
     4801 atggacggat ccagggtaga gattcacccc gatgcggcgc cggatttctt cgggtgggca
     4861 attcccatcg gtgcgggaag agcgagggta gggctcgcgg ggaggactgg tgtccgcgag
     4921 aggttcagga gttttctatc gctcatttca caggattcgc accaggcgca agtgcacctt
     4981 gtcagcggga ccatcccgct cgggaccatg gccaggacct ttggggaccg ggtgcttttt
     5041 gtcggggatg ccgcggggat ggcaaagcca acatcagggg gaggcgtgta taccggtgtc
     5101 agggccgcac gccacgccgc gagggtggca ggggaggccg ttgccttgga ggatttcggc
     5161 gacgggatgc tctcccggta cgaaagagcc tggaaggagg attttggaag ggaacttgag
     5221 caggggatgc aggtcttccg cttaaggcag cagatctcgc cgggggacat ggcaaagatc
     5281 gtctcagccc tctctgatcc cgcaatcgtg gaagatattg tcgaacttgg ggatatggac
     5341 cggcccggca ggttggcccg ccggcttctc acccgcccct ctctctatcc tcttcttggc
     5401 atactcatcc gttcgggagt acgtcttatt tacgaatagt aagatttttt atctaacaat
     5461 taatacgatt gggctagtca gtaatactct ggtgaatatc gtgcacattc ccgatgcgtt
     5521 tattccactc acccaggcag cggtgtactg ggtcatcgcg ctgatcttta tcgccctctc
     5581 actcaggtgg gccaggaacg agatgaaaga agagaaagtt cccctggtgg cggtcctcgc
     5641 tgccggcata tttgccatcc aggctgtcaa tgtgccgatt ccctggggaa cgagcgggca
     5701 catgatgggt gcggcccttg cggcaatcgt gctcggctcc ccctttgcag ccatcttcgt
     5761 cctcacgctc gtccttattg tacagggagt cctctttggg gacgggggca tcacggtaat
     5821 gggtgcaaac atcatcaaca tgggagtgat agggggcttt gtgggtttct acagttacaa
     5881 gggactgaat tccgccatca ggaacccgta ccctgccgcc ttcatcgccg gatggctctc
     5941 gctcttcgtg gcggcgctcg tatgcgcggt ggagatggcg attgccggga cctttccgct
     6001 cgaaatcgga ctgatctcca tggggcttta ccatgcggtc atcggggtga ttgagggcgg
     6061 aatcaccgcg gtagccctct acctcatcta caccgcaagg cccgacctga tcagcgacaa
     6121 cgcgggggtg gcagcctgat gggccgggag aacctcatct acttcggggt gatcattgcg
     6181 ctcgtcatcg cggtggccgc cccgttcgtc gcgtcaagca accccgatgg cctggagagt
     6241 gcattcttcg gggtctttgg ggcaaaggag gtccaggggt cggacctgga cgaggaagcc
     6301 gccggggccg ccgaggaaca ggtgcaggag gtcaccggga acacgttctc gtttgcgtcc
     6361 ccgttccccg actacagcat tgaagggatg gagaaagcgg gtgaggcatt ggtcatcgtg
     6421 atcgggaccc tcctcgtgct ggcaatcgca ttcggtctcg ggcgggtgct ctcccgttcc
     6481 gaataaaaga atacctctat tatttttcac gtcttaacgt cgcgggatac aaattccatg
     6541 cacctgatcg agaccaggga tctctcctat acctatccag gaaacgtcag aggactggac
     6601 cacgtgaact tcatcgcgcc ccggaatgcc cggatagcgg tgataggcgc gaacggggca
     6661 ggcaagagca cgctcttcaa gcacttcaac ggcatcttca gacccacgag cggaagcgtc
     6721 ctggttcacg gcgaaccgat cacccgcgaa aacataaagg aggtccgaaa gttcgtggga
     6781 gtggtctttc agaaccctga cgaccagatc atctcaccca ctgtagagca ggacgtggcc
     6841 ttcgggccca ccaacctcgg cctcgacgaa gagactgtca atcaccgggt cgaggaggca
     6901 ctcagggtgg tcgggatcga gcacctgaaa ggccgggttc cccaccacct gagcgggggc
     6961 gagaagaagc gggtggctat tgccgggatt atcgcgatgg aaccgcaggt tctcgtcctc
     7021 gacgaaccca cggcgggcct cgatccgcag ggggtgcgtg acctcgtcgg gttcatcaac
     7081 tccctctcgc ggacatacgg catgacggtg atcttctcca cccatgacgt atctctcgtc
     7141 ccggaagtgg cagatatcat ctacgtgatg caccgcggaa gggttgtggc tgaaggggac
     7201 tgccgggaga tcttcggcag gtctgacctc ctctcatcgg tgaggcttga tgtgccggtg
     7261 ctcccaaaac tcatcagcag cctgcgtgac cagggggtgg aggtgagcat ggcctataca
     7321 ttcaacgagg ccgaacgcaa cttccttgcc gcgtacaaga ggaatggatg atcgaagacc
     7381 tgttctttat cgagaagcag acctataaaa agagcctcat tcacgacctt gacgccaggg
     7441 tcaagatagc tgtctgtttt gcggtgattg tggcgatggt ggcggtcccg tattcgcccc
     7501 ttgtcttccc ggtggcggtg acattcgcgg ttttttttgc ggcactctgg gctctctcac
     7561 gcctctctcc cctcgcgtac gtgaagcggc tcgtaatgat cctcccgttc ggcctcttca
     7621 tcatcctcgt gcaggtattc gtgcagaacc cctattacac ggtattcaca cctatcgcgg
     7681 accttcccat ggggctgcac atctaccgtg agtccatcga gtttgcgacc atcctcctcc
     7741 tcaagttcct cgtctgtgtc tccttcatca tcctgctctc gtccaccacg cgggtccagg
     7801 atctcctgga gggggcaggc agactcggcc tgcctgcaga gttcaccctg acgctcggta
     7861 tgatgatccg gtatatcttc gtcttcggat acatgtacag gaagatcaac gaatcgcttg
     7921 ccacccggta ttttgtgccg tttgattcgc acctccctta tcggtaccgc ctccggacga
     7981 tcgcctacac catcggcacc ctcttcatcc gctcctatga acagggcgag cggacttaca
     8041 cggccatgct ctgccgggga tacgggaggg agagccatct ctatatcaga aaaaagccac
     8101 tgttccggaa ggaccatgca ttcctggcgg cgggtctcct cgtcgcagta tgcaccccca
     8161 tcttcttctg ggtctcctgg atcggcctga tctgatcgca cctttttttc cttcccgcga
     8221 cattcatcct catatggcca gtacattcgg gtatggattc atcacgaacc tcatgcacat
     8281 ctgcaagcac ttctccttaa agcctgaaga ggcgttttac ggggcagcgg accacctgga
     8341 cggcttcgtc atccccgacc agttcaaggg gaccgagatc gaagagatcg cggacaggct
     8401 tcgcaagcgg atcgtctggc accagcctgg cacccttgat aaagaagaag ccgcggaggt
     8461 ggtccgtctg atcaacaggc tgatcatcgc aatcgacaag gcccttggga taaaagaccc
     8521 ggatctgggc gaattccact gactgcggcc cattcccacc ttcattatta agtggtcagg
     8581 agtcgaatac tgtatgtgac gtccgttttg cggcacgcag cgtatttttt cgcaggggga
     8641 agcaatggcc gaaacgtatg atgcctctca tattactata ctggaggggt tgcagccggt
     8701 gcgggagcgg cccgccatgt acatcgggag caccgactcc cgcgggctcc accaccttgt
     8761 ctacgaggtc gtggacaact ccatcgacga agccctcgca gggtattgtg accgcatctg
     8821 ggtcagtatc aataagaatg gttcagtcac catcgaggat aacgggagag gaatccccgt
     8881 tgaccggatg gaaaagaatg gaaaatgtgc gctggaagtc gtgctcaccg tcctccatgc
     8941 gggaggaaaa ttcgataagg acacctacca ggtgtccgga ggcctgcacg gtgttggggt
     9001 ctcggtggtg aatgcactct caagctggct cgatgcccgt gtatacagga acgggaatat
     9061 ttacgagatg cggttttcgc agggaaagac catttcacaa tgtaacgaac gcgaggagtc
     9121 gctcgaagaa ctgcgtgccc gtttgaagca ttggtacctg gaaccggaga tacccgaagg
     9181 atcggcaggt caggcgggat cgattcccgc aaaggaactg gcagacgaca gtaaacttga
     9241 cagaaaccag ttcctgcaga aattcggtcc gaaactatca ggaacgagga tcacgttcaa
     9301 ccccgattcg tcaatcttcg agacagttca ttttgattac gacgtcctcg cccacagatt
     9361 aagagaactg gcgttcctga acgcagggct gatcatcttc atcactgacg agaggaacgg
     9421 ggatcatgca aggtaccagt acctgggagg cctgtcggag ttcgtgagat atctgaacga
     9481 aggggccgaa tgcctcaatc ccgtacctat ctttatctca aaaaaggatg aggagaacag
     9541 gctcgagttc gatgttgcca tccagtatac caccgggtac gatgagaaga tcttctcgta
     9601 tgtgaattcc gtcaataccc gcgaaggggg gacgcatctt gaagggttca ggagtgcaat
     9661 tacccgcgct atcaatacga tggcgaaaag gaacggcctg atcaaggaaa gttcctccct
     9721 ctcgctgcgg ggggaggatg tcagggaggg aatgaccgcg gtggtctcga tcaagatggc
     9781 gaacccccag ttcgagggac agacaaagat gcgcctcggg aacagcaacg tcaaggggat
     9841 cgccgattcc ctcgtctact cggcactttc cgaatacttc gacgagaatc cgaaagtcct
     9901 ccagattatt gtcgaaaaag cgctctccgc ggcgaaagcc cgcgaggccg cccggaatgc
     9961 ccgggaactc gcacgccgga agggagccct ggaaagtggc ggcctccctg gaaaactcgc
    10021 cgactgctcc gagcggaatc ccgaaaagag cgagatctac atcgtggaag gagattctgc
    10081 cggaggcagt gcgaagcagg gaagggacag gaagttccag gcaatcctgc cgctccgcgg
    10141 gaagatcctg aatgtcgaaa aggccggcga gcaccagctc ctgaaaaatg cggagataca
    10201 gaccctggtg tccgcgatcg ggaccgggat cggggagaac tttgacatat cccgcgcccg
    10261 ctatcataaa atcgtgatca tgacggatgc cgatgtggac ggctcacaca tacggaccct
    10321 cctcctcacg ttcttccata aatcgatgcc caagctgatc gacgccgggt atatctacat
    10381 cgcacagcct cccctcttct ccgtctcgaa aggcaaggtc gagaaatacg catacaggga
    10441 agaggacatg aagagactcc ttgccgatat gggtgaaaaa ggcatctcgg tcaagagata
    10501 caagggtctt ggagagatga atgcccagca gctctgggat acgacgatga atcccgagaa
    10561 ccggatcttc aagaaggtca tgatcgagga tgcgatggag gcaaacgaga tattcaagac
    10621 attgatgggc aaggacgtgg aagccaggaa ggacttcatc aagcggcacg caagggaggt
    10681 gaagaacctt gacgtctgat ccggaccttc cccgggacga tgcagtgacc gagagggtgg
    10741 aggaggtaag catcgagaag gagatgaaga cctccttcat cgattatgcg atgagcgtca
    10801 tcatccagcg ggccatccct gatgtccgtg atggtttgaa gcccgttcac cgccgctcgc
    10861 tcttcgccat gtgggagatg ggcaacacca gtgacaagcc taccaagaag agtgcgaggg
    10921 tggtgggaga tgtcatgggg aagtaccatc ctcacggcga tgcctccatt tatgacacca
    10981 tcgtcaagat ggcgcaacca ttctcgtatc gccacacgct cgtggaaggg caggggaatt
    11041 tcgggtccgt tgatggcgat gcggcggcgg cgcaaaggta caccgaagtc cggctcaggc
    11101 cgtatgcaga ggcgctcctc gaggatattg agaaggagac cgtcccgctc gtcccaaact
    11161 acgatgaatc actcaccgaa cctgttgtcc ttcccgcaaa aatcccgaat ctcctggtga
    11221 atggcaccga cggcatcgcg gtcggaatgg caacaaagat gcccccgcac aacctgcgtg
    11281 aagtctgcac tgcagtcgtg gcatacctcg acaatcctgc aatcaccgtc gaggagctga
    11341 tgacatcgct gcccgctccc gatttcccga caggtgggat cctgatgggg gtcgagggag
    11401 taagaaacat cttcacgagc gggcagggac gcctcgtggt tcgcggcgta gcaacaatcg
    11461 aggaaccaaa gagcggttcc cggaacccac gtatcatcgt ctccgagctt ccctaccagg
    11521 tcaacaaggc gcagtggatc actgccattg cggacatggt gaagaacaag aggatcgagg
    11581 ggatctccga catccgcgac gagtccgaca aggagggcat ccgtgtcgtg ttcgatctca
    11641 agaaagacgc gatcccatct gtcgttctca acaacctcta caagaacacc cagctcgagt
    11701 cgagtttctg ggcaatcaac tacgcaattg tcgagaacca gcccaggctc ctgaatctcc
    11761 ctgaattgct cgatcatttt gtcaggcacc ggattgagat catcacgaag cgatcggaat
    11821 tcgacctcaa aaaagccctc gaaaaggtcc atattttaaa cggccttctg atcgcgcttg
    11881 ccaggatcga tgacgtgatc gctgcgatca gggcgtccga gacggttgag gacgcacgaa
    11941 atgccctcat caccaggttc gggctcgacg agccgcaggc aaatgccata ctccagatgc
    12001 agctccgccg ccttgccgcc cttgaacaga agaagataat cgacgagaag gcagggcttg
    12061 aggcagagat cgcccggctc cagcacatcc tgtcaagcga ggagaccatc aaggcggaga
    12121 tccggcgcga gacgatggag gtctccgaga aattcgggga cgcccggagg acccagatat
    12181 cgctgaactt cggggacatc tcaaacgaag acctgattga agacaagacg gtcctcgtct
    12241 ccattactac cgcaaattac atcaagcgga ccgatatcga cgtgtaccgg aaacagaggc
    12301 gcggcgggca cgggatcacg ggaatgacga ccaaggagga cgacgtcgtc gattcgatat
    12361 ttactgcgag cctcaaggat tacctcctct gtttcaccaa taccgggcgg gtttactggc
    12421 tgaaagtcta ccagataccg gagaccagcc gtactgcgaa gggaaaaccc atcgtcaatc
    12481 tcctcaatct caaggacgaa gtggtaacca cggtgatacc cattcgtgaa ttcagggaga
    12541 accagttcct tctgtttgcc acgaaactcg ggcagatcat caaaataccg ctcaaagatt
    12601 tctccttccc gagatctgtc gggacgaacg ccatcaagtt gcgcggctcc gatcagctgg
    12661 ttgaggtcat cctcaccgac gggaacaagg agatcatcct gacaagccgg ttcggccaga
    12721 gcctccggtt ccacgaggag accgtgcgga cagtggggag gacagcccag ggtgtcaggg
    12781 gcatgaagat gaagggcggc gatacggtcg ttgcggtcac gcttgtggag aaggatcacc
    12841 ttctcacgat ctcggatgtc gggttcggca agaggaccga atttgatgag ttccggggcc
    12901 atggcagggg aacgatgggg gtcaggaaca tccagctgga acgcgatgcc gttgtcattg
    12961 cgtcatgtgc ggtgatggat accgacgaga tcgtagtgat gactgcctct gggatagtaa
    13021 tccggattca cgtcaacgaa atacgagtca tagggcgcgg gacgaaggga gtaaagatta
    13081 tgcggctcga cgaaaacgac cgcattattg gaattgcaat cgtgccgcag gatgatgaga
    13141 gcgggcagaa cggaacggaa aacgggacct ttacacctga tgacgagtaa ggccctctat
    13201 ttttctcccc acccccacac attttcatca tattcgacaa ttctcgcatc gggaaatttt
    13261 tccagttgaa aaacgcgaga ctcccaattc ccggacgaga tacgccttat ccacccccct
    13321 tcgcccgcaa atggcggccg gcaggctcac actgcacccg ctccatcaca ttcatccctt
    13381 cccgctccct cctctcctcg atgtaccaaa ctacccttca cctctccacg tgtggagagg
    13441 gagacatcgt ggatatcacg ccccggatac gggaggaggt aaaaaccagc cgagttgcgg
    13501 aagggctggt caacgtcttt gtcaccggct caacggcggc cgtcacgaca atcgagtatg
    13561 agcagggagt gctttctgac ctgcagaggg ccctctcggt ggtggcccct gacaacatcc
    13621 cctatgccca cgacagccgc tggggagacg ggaacggcag gtcgcatgtc agggcagcga
    13681 tcgtcgggcc ctcgctcacc atccctgttg ccggaggcga gccgttgctt gggacctggc
    13741 agcaggtggt gctgctggaa ctcgacgtgc ggaaagagcg cgcaagaacg gtggtgatca
    13801 cggtgatacc gtgacacgta aaagagaata ttctcacaga gccgggtttc tgataaaaaa
    13861 gggttgtaaa gtgttaatcc aggggaatcg tctccagctg ggagaccagt tcccatatcc
    13921 gggtccgggc atggaccggc atgtttgggt cgttgctgat ctcgtcgatc ttggagattg
    13981 cttctgcggc acggagcccg gagttttttg aattgttcat cagcagctgc cgggtctcgt
    14041 ccgcgactct tcggatattc ctgggtatgg tgctgtcgtc catgatgtgc tggagcatct
    14101 ggatacagct ctcgatggtc ttatcagggt tggtcatttg cactcccctc cggtgttctc
    14161 ctcgaaaaac tcctcaataa cgagtcattt tcggtccact atttatatcg ggagcaggtc
    14221 ttataaacca tccgacatgg tcgcccggcc gcagtgcccc ttcgcaaagt cgatatgggt
    14281 ggcccgcaca cctgcctacc acagagcgaa tggtggatct gatgaagcgc gaagacgaga
    14341 tcatggccga gcatctcctg agagggggaa agatgctctc ggcgtcctgt ccggtgtgcg
    14401 ggtgcccgct cttcgaggtg aagggaaaga ccctttgcgt ggtctgtgcc gaagggaagg
    14461 cggaaaagga agaggagaac caggctgtca ttccagggca ggcaacggcc gcacaggctc
    14521 ccccacctgc cgcgtcatca ccccgcccca ttgcatcctc ccggcttgca gacgcgctcg
    14581 aggagaccct cgtcgcactc tgcgcaagga ttcgcgagga gaaggacccc aggtgcgtgg
    14641 cggcactgat ggaggccgtt gtcaccggga tcgagggact tggcgccctg cgtccccggt
    14701 aaccttaccg gtacggcctg cgtgcaagtt cgtgtatctt ttttgaccgc tgctccccga
    14761 tgcccctgat cgtgagcagc tcctcttccg ttgcattcac cactcccctg acagttccga
    14821 agtggtccag gagaagccgt gcgttcttta acccgatgtc cggaaaggag gcgatcacaa
    14881 gttcctgctg ctccctcaag gaatgcccgt gacgcttcat ggggagggtc cgctcccgct
    14941 cgccgccctc ctccctccgt gcgagcacga ggatcatctg tgccgtgtcc tcctcgtcct
    15001 tcgtgaagag gatgctgatc ccaaggtcga ccgcgatggc ggcaagggag cccctgacgg
    15061 cgtttgggtg gatgtccctt gccgtgtaaa ggtcgccccc ctcgatgatc aggacgggcc
    15121 tcggggcgct ctctgcaagg gccttcagct gcccgaagag gtcacgctcc accagggtgt
    15181 ccacgaagtc ccgtgcagtc ttcctctcaa tgaggatgcg gtccccgatc gcatagtctc
    15241 ctgacgggag ccttccgagc acgagggata cccccatgtt gctcaggatc tcaaccacct
    15301 tcgacgaagt ctcccggtcg tcaacccgga cttccgggcc ccgcccggca aactccccga
    15361 tactcgactg gacgggtggc tgcggtctct tcccggggag tccctgcgat gagggggaga
    15421 tatgctttat tcccgagagc attgagcgct ccctgttctg gctcacgtac cggaagacct
    15481 cgtcggaggt tcccttcgtg acaaggacga cgatcctccc cttcccgctt ctcccggtcc
    15541 gccccttcct ctggatgctc cggatctcgg aggggactgc ctcgtagaag accacaaggt
    15601 cggtcgaagg cacgtccagg ccctcctcac cgactgaggt cgcaacaagc acgcggaagt
    15661 cgccggcccg gaatttctgc agggcctcta tctggcgctt ctgggagagg cccttctcgc
    15721 tgtcctttgt cgcctggccc acgaagcgct ggcaggcgat tccttcggaa ttcaggtggt
    15781 ccacgatctg ctgcaccatg tcgcggaacg tcgcaaagac gatgatccgg ctctccggag
    15841 aagcttcgag ctgatccttc accactctgg agaggatctc catcttgggg tggagttctt
    15901 cattccatga catgcagcgc tccagaagcc ggatgaatac cgggtccctt gcgagccgtg
    15961 ccgtcgcttt cgggccggaa ccacctgacc cctccgagaa gagccgggtg atgtagcctg
    16021 cgagcacgcg gctgccctgg gactctgcga gcgagagcgc atgccgcagt ttcatcagtt
    16081 ccgcgtagat ggaggctgcg gcgtaccctg acgactcccg cgcctgtatc tgttcctgga
    16141 cctgcgcgtt gagtgcctgg agggacttca tggacagctg ctgacggggc gggacgcgga
    16201 acccgaggtg ttttaactgg gtgagtcgcg agtccacgag cgcatccagg tctgatgctg
    16261 ccttcttcag ttcgggcggg aggtcgactt ccacgacctc gatctcccgc tcgtggatgt
    16321 agggggccac gtcccggtcg tgctcggtcc tgctctcgac ctgtacgatc ccgaggttcc
    16381 ggcagacgtc ctcgaccttc tcaacctggc ccccgggcga ggctgtcatt gccaggacaa
    16441 ggggtgaggc tgcggtctcg aggtaccgct gcgccaggaa gacgtaagcg taatttccga
    16501 ccgcacggtg acactcgtcg aagatcatca gggagacgtc ccttgtgtcg taactgcctg
    16561 caaggacgtc gttttttatc acctgcggcg ttgcaaaaac catcctggcc ttcttccagg
    16621 cggccgcacg ttctcctgaa ggtgtctccc ctgtgaacat tgccagggta tcgtcttcct
    16681 gcgaggggag cgagaggaac tgccggaaga agcggagatg ctgctccacc aggggcttgg
    16741 tcggggccat catcaccacc ctgccgccct ggttgtagag cctcgaggcc gcgacaagaa
    16801 gtgcgatggc ggtcttcccg agtcccgtgg gcagcacgac catggtgtgc ccctcgagcg
    16861 ctttcatcgc gatggagagc tggtaggtgc gcgactccag cctgccgggc gcaatgaagg
    16921 ggtgggtgat ggtgttcatg caggggagag ggatgcgaag gttgcggacc cttccacgag
    16981 ggaccggatg agatctggga gggtttcggc cgggacacgc acctgctcca tgctgtcgcg
    17041 gtcccggaga gtcactgtcc cggtctcggg agtttcgtaa tcaacagtga tggcaaaggg
    17101 agtcccgacc tcgtcctgcc tccggtaccg acgcccgatg gctccggagt cgtcgtattc
    17161 agccattatc cccctggctt tgaggctggt gacgatatca cgcgcgatct cggggagccc
    17221 gtcgcggttc atcaggggga agaccgccgc ctggattggg gcgatgcgcg ggggaaggcg
    17281 caatacccgg cgttcctcgt cgtttactgt ctcctcggca tagcggtgct cgagcaccgc
    17341 gtagatcatc cggtctatgc cgtaacttgg ctcgatcacg tggggtgtga tctcctctcc
    17401 cgggatctca acctcctcct ccctgacctg gtagaggtcg ggggagatga agacctcctc
    17461 gccatcgatg gtgagggtga ctccgtccgg gccggggacc acgtctgcca gtgcgtctgc
    17521 aaccgccttt gccttccccc tgaaccttgg gccgagggcg cccatgttgg cgaccacgcg
    17581 cacccggcgc tccttccgcg gggtctcgta aggaatgaac accgcaaatc gctgcccgct
    17641 ctgcttctcg tgcgccctta agtcgtagtc ggtgcggtcc gcgataccca cggtctcaac
    17701 ccagccaaac cgctccgaga agatctccgc gtcccagcag tcctctgcat agtgggcgcg
    17761 ttcgtccggg aggtgctggc ggaagcggag tttctccgga tcaaccccca cggtcacgag
    17821 aaggtgatgg gtgagggcaa gataatatgc cacgtactgg ttggcgatca gcccctcgcg
    17881 aacggcctca tccatggtgg tctccagggc aggggtctct tcctgttgct ggcgtattcc
    17941 aagcaggggc atgcggtacc cggcgtaccg ggagaagaac ggatggtcct tctcatctgg
    18001 atggacaaat atctctgcct cagcctgggt gaactcccgg aggcgtatca tgccctgcct
    18061 gggggagatc tcattcctgt aggacttgcc tatctggatc gccccgaacg ggagcgtatc
    18121 gcggtagaaa cgcaggaggc gctggaaatc ggtgaacatc ccctgtgcgg tctcggggcg
    18181 gaggtaaccc tttctctggg aaccggggcc aatagaagtc tggaacatca ggttaaaaaa
    18241 aaagacctcc acctcgccga gcacctcgcc gcagacagga cagggtaggg tggagagcac
    18301 gcgggagagt tcaccagtgt ccatggtcga ggcccccggg atcccctgcc cttccgcgat
    18361 atggtcggca cggagatact ccctgcagtg ggggcactgg cacatcttgt ccgagaaacc
    18421 cttcacgtgc cccgaggcga tgaagatgtc ctcctgcccg atggtggggc actcgatctc
    18481 gtagtacccc tcccggacga catagaattc cctccacgtg tcctcgattc gcctcttcat
    18541 catggagccc agagggccgt aatcgatgaa tcccgcgacc gaaccgtaac attcggacgc
    18601 cggccagata aagccccttc tcctggccaa atccattatt ttatcgtata tatcgctcat
    18661 ggacacactt ctctcttata tctggtgctg cggccagaaa aactgtgcgg gaaggcgcat
    18721 gtctttcggg ggagataccc ttttctcaga tcccagggaa ctgcctccat atgtccgtat
    18781 ctccatgata aagccttaaa gctggagagc ggggcagaag gatagtgtta tatatattgc
    18841 tgggtatctt attcgataat tcacgagcga ggtttcatgc aggacgacaa gagaaagaaa
    18901 gagctgctcc agttgttcac caatatatca gggaaaacaa agatcgtgga acccatgaaa
    18961 aagatccacg ggaccctcag ggaccgtgat gccgtggagc gcgagatcgc gctggtcatg
    19021 cgggagatcc tggaacaggg tttttttaag acaaaactca agcccatcca gctggcaagg
    19081 ctggtatctg cgtattacgc ggggaagaat gataccgaga tagcccgcga gctcggggac
    19141 gagaaacttt caaagacggt tgcccgtgcc agggtgaggc tcaagctctt tcgcgaactg
    19201 gacttcaaga tgccgtttga ccaggaccgg atggaggatc tcctcatctc cggcaagaca
    19261 atgaaggagg tgagcgagga tctcggggtc agcccgtcca cacttcggga ataccggcat
    19321 gtgatcgagc agagggacga tgtcacgatc gatccattcc tcgagcggat aaaggacgtg
    19381 atggaggaca gggacctctc cgagcagatg acccggagcg ccaccaagga cagcctcggg
    19441 gaggcaatcg acatcaccga ggcagagatg atcgacgtca cgtgaagatg ccaataccct
    19501 ttttttcccg cgccaaccca atatcccgta ccggggcata gagggaggga cccagatgaa
    19561 actcacatgg tttggacatt cgtgcgtgct cctggaaggg agccggaaag tgctcattga
    19621 tccgtttatc cctgaaggat ccattgatgt ggaaccggac gtggtagcgg tcacccatgg
    19681 gcatgccgat cacatggggg atgcggcgtc atatggagtg ccggtggtcg ccatcaatga
    19741 agtggcgaag tacctgcgga cgaaaggtgt tgaggcagag gccatgaaca tcgggggcac
    19801 gatcgaggtg aagggcgtgc ggttcaccat gacccctgca ctccactccg cctggatcga
    19861 agaggccggt atcggatgca acggcggctg tgcggcaggg ttcgtcattc gaatggatgg
    19921 aaaagcaata tatcatgccg gcgatacggg gctcttctcg gacatgtccc tgatcgggga
    19981 actctaccac ccagatgtgg cgctcctccc catcgggggg aggttcacga tgggcccgga
    20041 ggaggccatg gtcgcggccc ggatgatcgg ctctcccctg gtgatcccga tccattacaa
    20101 cacctggccg gcgatctcac aggatgcgga agggttcaag cgtacgctcg aaaggacaac
    20161 tgactgcagg gtcagggtac ttgcaccggg aggatcaatt actctttaaa ggaacgttcc
    20221 cgtcaagagt tcggggagta ctcccgaaca tcccctgcag atgcacgata catttctcgc
    20281 agcgaaccgg attaaaaggg aaccctcatc agatatcctt atcttgtccc actggaattt
    20341 tgatgcatgg aagagtgtgt gtaagatgga acttgacgga aagacagtga ttggtgcagg
    20401 cgttgtcctg tttgtgatcg tagtcgggat atggtacatg ttcctcaggt gacggaagcg
    20461 taacagatcc cagttgaaga acaacacggt ccctcattta ccttttttca tccagttctc
    20521 ctgtatcccg caggtatggc gatccccata agaggctgcg cgtaaccccc gcattggaac
    20581 cggattaacg gcatgcgatg ttgataattg taatctataa ccaactaata gtaaggtata
    20641 tttaacaata tgttaaacct aatgggcata tgcacgtgat aacaggtgaa tatcaaatta
    20701 ggaggtccat cccaaggatc tcatccctgg ttcttgtgct tgcgatatgc acattctgca
    20761 actgcatgat tgcagttgcg gacgccacga atggtgaggg aacggagcag gctatcggaa
    20821 tagacatgtc cgatgcagcc catagcagca aggcagaacc caattacgaa gtggtctttc
    20881 ccgccgatcg ggtcaacatc atgacgatca ccatcactcc gggagactgg gagaaaatgc
    20941 gtgagaatat gaccgagatg tacggcgagt ttggtgaagg aatctcaggg ggaggtcccg
    21001 ggcaggctgg tgagccgggc ggcaatccgg gagaaagaca aaaaatcgta ggaatgttca
    21061 gcgaggttga cccggtctac gttcctgccg atgtatcgtt cgagggagtc ggctggcaga
    21121 atgtcggtat ccggttcaaa gggttcaatt cgctccaggg cgcatggagg gaaggttcct
    21181 acaagatatc gctgaagctc aatttcgaca agttcgagga tgaattcccc gagatcaaga
    21241 accagcgttt ttacggattc gatgaactca acctgcaggg aaatttcaat gatgactctc
    21301 tcttgaggga gatggttgtc ccttcgatat tcagggagtc cggcgttccc gcaccgtata
    21361 cggcatttta tcgcctttat gtggactatg gggatggccc gaagtatttt ggtgtataca
    21421 cgatggtcga ggcagtcgag gatacattga tagaaacgca gtttgcggac gactccggga
    21481 acctgtacaa acctgaaggc aacggggcta cctttgcggc cgggacattc gatcgggaca
    21541 gtttcgagaa gaagaccaac gaggatgagg cagactggag cgacgtggag acgctgtacg
    21601 cggtccttca ttcggacatg cggctttcaa atccggtgaa atggagggcc gagctcgaag
    21661 agatcctcga tgtggacgtc ttcctgcgat ggcttgcgac caataccgtg atccagaact
    21721 gggatacgta tggtggcaac tgcaggaact tctacctcta caccgacccc gccgatggga
    21781 ggattacctg gatcccgtgg gacaacaact acgccctcga taatggtatg ggaggaggag
    21841 gccaggttcc aggaggtacg aacccgggag atccgggagg gaggaactgg acaccagggg
    21901 gtatgaaccc gggagagatg gaaggaagga gtcggactcc gggggagata aatccaggag
    21961 acctgggaga gaggaactgg actcccgggg acatgaatga aacacccggc gttggcatgg
    22021 gcagcacact ctctcttggt cttgatgaag taggtgaaaa ctggccgctt atccggtata
    22081 tcgcggatga cccggtctat tatacccggt accaggagta tctcggaata gtggtgacga
    22141 catcatttga accctcgaag atggaggaga catacgagta ttaccatgcc cttatcgaac
    22201 cgtatgtgac ggggcaatat ggtgaacagg caggatacac ccacctggac aatccccggg
    22261 atttcgatac cgcgctcgac gacctgatcg aacatgtgaa gaaccgctat gacgcggtga
    22321 tggagtttct cacccttgct cctgtcgcga aacccacccc cacacctact atggcgggtt
    22381 tgaaggatct cacgcctccc accgtccgca taaccacacc tgcacagtcc gctcaaacag
    22441 cgcccgggga ggcacttcag attgcctggt ccgcaaccga taacaccgga gtggaaggcg
    22501 ttgccatctt ttatacactg gatagaggca agacatggat ccagattgcg gacgacctcc
    22561 cggaggaagg atccttcaca tggacggttc caaaagatgc aggctcgaca ctgatcgtga
    22621 aggtgagtgc ggccgatgct gcaggcaacg tgggatccgc gagcagggtc tgcacatgca
    22681 catcagccac cacaacagca gtgttaaaag gcgggaacct cattccctca ccaataaaac
    22741 ccgtttctag cttttctgat tcatggaaca tcaaatctct tcagaacggg aatacgaatt
    22801 cggaattcat cgccaacagc cctgaatcgg cccggtttct cagccccgtc acaaagcctg
    22861 tcacactgtc gttgcagaag gcggccacgt tctcatccca atctggaagg gacagtcttt
    22921 caagtggcag gggatgagtg tgatcgagat gaacgaagaa accgccggca tgcagtatgg
    22981 accaactcat tgcgctggcc ccggaagcag aatgctgact gcaacgcggg atttcgggag
    23041 tatatccctt aaaggtctgg aagaagcaaa ggccggactg ctcatccggg aggagagcaa
    23101 gcacctgatg acgctggagc agtgcctggc cttcctagca ggacttccgg actcctattg
    23161 cgtgcttgag gtgaacaact cgagaattac acgatatgag actctctact acgatacacc
    23221 ctcgttcctg accttcctcc agcaccacaa cgggaaaggc aaccgctaca agctgcgcat
    23281 ccggcgttat gactcgtcca aagagaccta tctcgaagtg aagaagaaga ccagcaaggg
    23341 aacgaccgag aagagccgtg tcaggacctg gtggaccaca gccggcttca cacccgagca
    23401 ggaggagttc ctggaggccg cgtttcccta cgattaccgc gagttctttc cggtggttaa
    23461 aacagtatac gaccggctga cactcgtctc tacgaaatcc cccgagcgca ttacgctcga
    23521 ttcgaacata tcattcttga gcgggcagga gcagaagacc tatccgggac tcgttatcgt
    23581 ggagataaag cacgaaaaag ggatcagggc ctcaccagcc ctgtctgccc tccataccct
    23641 tggaatcagg aagagaggat tttccaagta ctgcatcggg gtatccctgc tctatgaccg
    23701 ggtgaagcac aaccggttca agcaaaacct tctcttgctt tcgaagcttg attcagtgag
    23761 gtgtcaggca tgctgatcga tgccacaccc ctcttcatcg cgggattcgt gctcaatttc
    23821 cttgcagcgt tcattatcgt acgcttcgta tactacccga gagggcggtg caataattat
    23881 gttttcacat tcctcgcgtt caacacggtg atctatttca tcatggggat gtttacaagc
    23941 gtcgagatct cgatcggtgc aggtttcgga ctgttcgcac tcttctcgat cctccgttac
    24001 cggaccgaga cagttcccat ccgcgagatg acctatctct tcgtgatggc cgccctcccc
    24061 atcctgaact cgatcttctg gcagtcagcc caatatgaaa agctgctgat cgtcgacatc
    24121 ctcgtgatcg ctgtcatctg gttcctggac cgcgaatggg ggttcaaccg ggagcagttc
    24181 cagatgaacg tgatgtacga gaagatcgat ttggtcaaga gggagaggcg ccgggagatg
    24241 ctggccgacc tctgcgagag gaccgggctt gcggtcaccg atgccgaggt gcagagcatc
    24301 aacttcctgc gggatgtcgc ggaactgaaa gtcacgtata cgaacaggag acatgactga
    24361 tatttt
//
LOCUS       DAOQYR010000025        22717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_2623_length_22717_cov_8.620113, whole genome shotgun sequence.
ACCESSION   DAOQYR010000025 DAOQYR010000000
VERSION     DAOQYR010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 22717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22717
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2623_length_22717_cov_8.620113"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PKM87_04740"
     CDS             complement(<1..476)
                     /locus_tag="PKM87_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ29409.1"
                     /translation="MAAAEHNMHNYREVYDHFSLKVPEYYNFGFDQVDRLAAKDRNRL
                     AMIWVDQEGHEKTFTFRQMMNLSNSAANMLLKYGIKKGDRVIVMLTRVPEWWIFALAC
                     IKLGAVFCPCPTMLTGKDLKYRINAAKIRMVITDNENAAKVEEICTECPSLRSRLV"
     gene            complement(603..2270)
                     /locus_tag="PKM87_04745"
     CDS             complement(603..2270)
                     /locus_tag="PKM87_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ29410.1"
                     /translation="MEGTTESQGCYEDLCRNFEIHVPKYFNFGFDVIDSWAEKDRNKL
                     AMIWVNQEKEEKKFSFRDMKNHSNQAANILIKYGIQKGDRVLLVLPRVPQWWVFVIAL
                     IKLGAVVCPCPTMLTPKDIRYRLNAGKFKMVITSLEHAGKVDEIAEECPSLSCRFLLD
                     GERPGWISYPHELFFPAPVSHHSVSMPVDKKTLATDPMLIYFTSGTTGEPKMVLHNHA
                     YPLGHLVTAGLWQDLTQNDLHFTYSDTGWAKCAWGKIFGQWIQGACLFVYDVRGKFKA
                     TELLPLMEKYELTTFCVPPTIYRMLILADLEKFDLRELRRCVSAGEPLNPEVVRVWQE
                     GTGLPIAEGYGQTETVCCIGTFRGMKVKPGSMGRPAPGWHIELHDEEGNRVPKGEEGR
                     IAVKLDPRPVGLFVEYLNNPEANAESFVNGFYYTGDKAYMDDEGYYWFVGRDDDIIKS
                     SGYRIGPFEVESALLEHPAVAESAVVGSPDLIRGTIVKAFVVLKPGFEPSERLVKDIQ
                     THVRKNTAPYKYPRAIEFTHDLPKTLSGKIRRGELRVRELERFNSSR"
     gene            2398..2952
                     /locus_tag="PKM87_04750"
     CDS             2398..2952
                     /locus_tag="PKM87_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HNQ29411.1"
                     /translation="MVGSRYESLKIENIVASGAIADAIDLADVSKKIEHCELNQKRFP
                     GAVFRMENPKIASLIFSSGKVVLTGIRDQDSLDKGLSMIIAAMKEAGVDTYPEPRVGV
                     TNIVCSYDIGKNINLNKVVITLNLENIEYEPEQFPGLVYRIKDPKIVALLFSSGKIIL
                     TGGKTLDEIRRGLDFLEQKIGGIL"
     gene            complement(2953..5646)
                     /locus_tag="PKM87_04755"
     CDS             complement(2953..5646)
                     /locus_tag="PKM87_04755"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNQ29412.1"
                     /translation="MEILRTLDRRVQECIRSRGFSRLSEIQENAIPLVREGRHLLLIA
                     PTGTGKTESAMIPVFDAMMSHTGSGFCTLYITPLRSLNRDMLQRLEWWCRELGLSVGV
                     RHGDTPQSERRKQALHPPDLLITTPETLQALFMGKVLRKHLSDVRYVVVDEIHELAGS
                     KRGAQLAVALERLKEYAGEFQRIGLSATVGNPEDVGRFLCGARDFSIVSVPVAKHLEI
                     SVDFAGDSFEKQAAWVSRAIGRHESTLVFVNTRVTAEALGHHLYHRGDVEVHHGSLSK
                     EVRIEAEERFRSGAVRALISTSSMELGIDIGRIEHVIQFGSPREVARLVQRVGRAGHQ
                     MDAVSRGTVLATGFDDLLESLVIARRAKSAQVEDVSLHLNAADVLANQVAALAVEYRE
                     ISRQKIRDIVERSYPFAMAGNLVDEVCTQMAEHGLIRIEGDMVRSGARSRRYLSSNLS
                     MIHDERKVVVFDMVSRKTVGTLDESFVVGFLHTGAVFITKGQLWRVLDMEEGKVTVEP
                     AKKAKGELPSWEGEQIPVPFSVAREVGALRRSRDFSPYHAREEPLGFAHRFLVEMDRN
                     RSRVPDDTLITLENAPEGVVCNVCAGHKANEALARVLSILLSARYGTTVGIEIDAYRI
                     YLRLPSAVKAADVREILLSLDPIHLEGILKLAMKRTALFKWKMVQIAKKFGAIDPDAD
                     YERISTHRLVEIFDGTVVQKEAYRELFSVYMDVPAAAQIVSLIRDGSIDVAVSPLSIL
                     GAEGLFASRDQISPPTADQAVIGTLKRRLEQQEVVLACMHCRKWKSRTTVARVPDPLR
                     CPLCGAGLVAVLKPWEEPLVALAKKKKTQNEEERATEQRLLRNANIVLSGGKKAVTAL
                     AARGVGPENASRILSTLAEGDGFYREIIKAERNYIRTRRFW"
     gene            complement(5624..6391)
                     /locus_tag="PKM87_04760"
     CDS             complement(5624..6391)
                     /locus_tag="PKM87_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ29413.1"
                     /translation="MRLEFLNAGPALLVEGDVRVLVAGDLHLGIESDLSRHGLHIRSR
                     SRERKERLIACIEAADPELFVLLGDVKHNVPLTSRQEYRELPAILDAFRSKVPLRLLP
                     GNHDPGIEKFFHDDEVLPKTGAVIDGVGYIHGHTCPDPSLQGHLIIAGHHHPMVSIRD
                     EVGCALRAPGYLLAPLDDACLQFARAADTQGEETRVLFIPALNELAGYDIMRTVREPF
                     SPLSRCIKAEQSEVFLADGTFLGPLSSLEDHGDTPDA"
     gene            complement(6388..7164)
                     /locus_tag="PKM87_04765"
     CDS             complement(6388..7164)
                     /locus_tag="PKM87_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29414.1"
                     /translation="MNGTYQRVFAREYNGSIATFTESGTHSPVAVLTPGGAICTTLFC
                     AGAVLERQGRKGEFMHARLADSTAAFELHIDRTDQVTADILESMEIPCFATVIGEARI
                     TGKGGAGRCTVRVIEMREVDRYVRDAWVLRTADLTMRRIEDLRTALDEGRRDARLDAL
                     VLHYHLDASRLRDLALMVKEAIKGVNAPHASSPEAGDPVALVLEIIREHGGKTGISLE
                     EVTRKAAKHGLAASEVKQAVESLLLEDECYQPARGVLKVL"
     gene            7430..>10040
                     /locus_tag="PKM87_04770"
     CDS             7430..>10040
                     /locus_tag="PKM87_04770"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNQ29415.1"
                     /translation="MRTERLRWLVILVSLFITVVSGTIGFPLVDLHIGDAGFGNELPG
                     IAPSVFDGGERGLQERVTTMPLLFIENAGQSDDEVEFVVISDGGSIFFTREGVTVKLV
                     TLMEDTPAATLVGYRFVGAEPSPRITGLDPLEGKVNFLVGNEPDGWRTGVPTWRAIRY
                     HDLYRGVDLVYEGTPEGLKSAFTVAPGASPGDITLEYSGILGLVLAEDGSLHIKTSAG
                     ELVESAPVCYQEIDGEVIPVGACYVLKGSSRVGFMIGPYDQTCPLVIDPVMKYGLYLR
                     GIGVAYGRSVALDDTGNAYLTGETFPAPYTMSPGSNDMSGDGTDVVVAKINPDGTVPL
                     YVTYIGGDGDDIGRGIRVDAEGNAYVTGQTASADYPVVNAVKSTLSGPSDAFATRLSQ
                     DGATLVFSTYLGGAQDDAGNAVALDSAGDIYIAGNTMSSDFPGIRVPHNTNYGGNQDA
                     FILKMSGDGSQVAFFEFIGGSKSDSGYGVAVDSEGNSYVTGETFSLNFPVINAMQGSL
                     AGGRLSDAFITKVSPDGSSFVYSTYLGGSQVDSARSIGVDAKGRAHVTGATILGVFPV
                     KDAFQPVAGGCTDAFYSRLSPDGSSLEYSTYIGGVNIDEGKGVAVDECGVAYVTGLTF
                     SPDLPVINPYQRFLGGESPFSVEVSDAYVAKFVPWDTIPEYVTYLGGDGDDVGAAIAI
                     NRQACVDCIAERTCASITGYTDSTNFPVSDPYPISFQPKGEGGFLFTLCDDGESPGMP
                     VADFTAEPTSGCPAPLLVQFTDLSTGIPTSWYWEFGDGATSTLQHPTHTYTTPGTFNV
                     TLTVTNVCGSDSLTKYEYISICPIYVPVANFTAEPTSGCPAPLLVQFTDTSLHDPTSW
                     NWSFGDGSFSTLQH"
     assembly_gap    10041..10140
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10141..13994
                     /locus_tag="PKM87_04775"
     CDS             <10141..13994
                     /locus_tag="PKM87_04775"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ29416.1"
                     /translation="VTLTVTNEAGSDSLTKHEYIYVCPFIPPVADFTAEPTSGCPAPL
                     LVQFTDTSLNNPTSWNWSFGDGSFSTLQHPNHTYTTPGTYNVTLTVTNEAGSDSLTKH
                     EYIYVCPFIPPIANFTYISECPDSEFVRFTDTSLHDPTSWNWSFGDGETSSLQHPNHT
                     YAETGGYNVTLTVTNEAGSDSTTQIICVVPCIDPPVANFTYISECPDSEFVRFTDTSL
                     HDPTSWNWSFGDGSFSNLQSPTHNYAETGGYNVTLTVTNEAGSDSTTQIICVVPCIDP
                     PVANFTYISECPDSEFVRFTDTSLHDPTSWNWSFGDGSFSNLQSPTHNYAETGGYNVT
                     LTVTNDAGSDSTSQIICIPECPGPVDPPVADFTYVSECPDSEFVRFTDTSLNNPTSWF
                     WTFGDGETSDEQNPTHTYSSTGTYNVTLAVSNSGGSDSITKNVYVPYCPGPVDPPVAN
                     FTFCSNPSDPLTVYFTDTSLNDPTSWFWQFGDGTTSNLQNPSHTFPAVSGYEVTLTVT
                     NEGGTDSISKILCIACIPPCPEPPVADFTYISECPDSQFVRFTDTSIGNPAAWFWQFG
                     DGATSTLQHPNHTYAHTGGYNVTLTVSNKGGSDSITRIICIPECPPPNPPIACFYASP
                     RTGYAPLEVEFCDCSETDILTWSWDFGDGGTSTEQTPRHIYQEYGSYTVSLYVTNATG
                     YDTETKADYIRVSSPLPGPPSAYFTANQTTGHVPFAVAFTDGSTGVPETWSWNFGDGG
                     TSTDRNPVHVYTMPGWYSVSLTVENSYGSSTYSRPEYITAQDSTGGDKGYFLVHCNVD
                     GAAVTFNDDYKGLTEDGTLLVMVYVTGTHYTHYTVGKDGYYPVTLPISTYPAKDQTLH
                     LYATLEPVPGDYTITATAGPNGAIIPNGTFTVPAGSDREFTVIPNPGYKVENLRLDGQ
                     GLGALTSYTLEDISDNHTLAATFVQNETVLHVISATAGPHGQIVPSGQILVPDGATAG
                     FAITPHLGYSISDVRVNNISVGMPSSYQFINVTHDNTIVAEFGKTNFSISALPTENGT
                     IIPSGVTEVAYLESFTYEIVPNSGFFTAQLFIDGESVTPAPTYTFDNVAANHTISAVF
                     LPDGSLTFYIQATAFTGGNIIPSGNVSVLGGNDITFTIYPDSGHVVQNVMVDGDSKGG
                     LLDWTFRDVAANHTISATFAPSGGGGGGGGGGGGGGYSVSTTTQPTTVPTTPVAETEG
                     YESGTSEETITPTPTATAEVTGVETIVTTVPTTIPPAEPFWTRFPLYWLVPLIIVILI
                     LAAVAYYYWKKKQQQEVLFEE"
     gene            14181..15149
                     /locus_tag="PKM87_04780"
     CDS             14181..15149
                     /locus_tag="PKM87_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C small subunit"
                     /protein_id="HNQ29417.1"
                     /translation="MNDTTMIWIEKYRPQTLADIVGQDEIVERLQSYVKTGNLPHLLF
                     TGSAGVGKTTAAVALAREFFGESWNLNFRELNASDERGIDVVRNQIKQFARTSPLGGA
                     TFKILFLDEADALTPDAQAALRRTMESYATTCRFILSCNYSSKIIDPIQSRCAIYRFR
                     SLSGEAITKEILRIAENEKISITEPAIQAIVYIAQGDMRKAINALQGAAILAAEIDAG
                     MVYAITATARPDEIEDLLATSLSGDFEGAEAILHHLLQDRGIAPNELINQCYRTIVKR
                     DMDPELRVALIDQLGTTDFRLSEGAGTEIQMEAMIAQFVLQAKKHG"
     gene            15142..17283
                     /locus_tag="PKM87_04785"
     CDS             15142..17283
                     /locus_tag="PKM87_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minichromosome maintenance protein MCM"
                     /protein_id="HNQ29418.1"
                     /translation="MAKRADSPAAEAASEIVDRDADWSRFLKSRYKKQLSELAREYPY
                     RRSLQIDYRELESYGRAGLRMADELLDNPGKVIEDVRNAIKNHQLVKGRDGKPTGEVN
                     VRFVNLPRKIGIRHIRSDHINRFISVEGILRKTTEVRPRIVQAVFRCPGGHITIKEQG
                     YGKFREPDGCATEGCTFKKLDLIARRSRFVDSQKLRIQESPEGLRGGEQPQTLDVDMT
                     DDLTGMVAPGDRVVINGILRSMQRVTHGEKNTLFDIYLECNSIEIAEKEFEEVQIDEK
                     DEEEILTLSRDPNIYRKIIHSIAPTIYGNEDVKEAIALQIFGGISKEMPDGSHLRGDI
                     HVLLIGDPGIAKSQLLRYVVKLSPRAIYTSGQSSTSAGLTATAVKDEFGDGRWTLEAG
                     ALVLADMGIAAVDEMDKMQKEDRSALHEAMEQQSISVAKAGITATLKSRCALLGAANP
                     KLGRFDAYLPIQDQINMPGSLLSRFDLIFKMTDIPDHTRDSAIAIHILKAHSIGETIA
                     QHKRTPIPGVDDAYIEEQLRPVTPEIEPALFRKYVAYARRTCFPRLGDAAREALIGYY
                     MQLRDLAAGSDKPVPVTARQLEALVRLAEASARIRLSPNIEAEDSERVIKIVDTCLRE
                     VAYDAKTGTFDIDKVVTGISKGRRDLIRVIKQTIRQIQKDTGNDRIAVAELIDLLTPQ
                     GFSRDEIDAQIKVFLREGEAMEPKSGFVKLI"
     gene            17324..17647
                     /locus_tag="PKM87_04790"
     CDS             17324..17647
                     /locus_tag="PKM87_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase family protein"
                     /protein_id="HNQ29419.1"
                     /translation="MHTAREKAAFEAGIKLGALYHQWVGTPVSPLTAASIESAIEKSV
                     GLQPHVKGISVRLDRDIMKPNVFGYSELQGLMLTVEIETRVDGEVCRARLRREGDYPM
                     MEIVE"
     gene            17648..18475
                     /locus_tag="PKM87_04795"
     CDS             17648..18475
                     /locus_tag="PKM87_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNQ29420.1"
                     /translation="MGGPRFPITDHHIHLDPRNGRGIDAAKDFRRSGGTHLFLVSKPS
                     SSFGILPVKGEDFLPVFEETLAVARMVSETGITVYPVLGVHPAEITRLMESFPLSRVV
                     EIMKEGIALAAGFVIDGAAVGLKSGRPHYEVSPEVWDAANDVLSYSFQKAGEAGCAIQ
                     VHAESGPCADMREMARAVGLSPDQVVKHYAIPETTLVPSLLATHPDIPALAMEGRAFM
                     MESDYMDELSRPGAVIGPRSVPRVTTRLLSTGQIDEDATHRIHVSTPSQVYGVDISL"
     gene            18525..18818
                     /locus_tag="PKM87_04800"
     CDS             18525..18818
                     /locus_tag="PKM87_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF424 domain-containing protein"
                     /protein_id="HNQ29421.1"
                     /translation="MYLKVHRSPAGDEVVAVCDRELMNTTLSGNEVEIRITGSFYGTE
                     LATPEEVSSALSRAENANLMGERTVALAVEMGLLSRSACLMFGDVPHAQLFRI"
     gene            18823..19863
                     /locus_tag="PKM87_04805"
     CDS             18823..19863
                     /locus_tag="PKM87_04805"
                     /inference="COORDINATES: protein motif:HMM:NF016842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_function: GO:0043023 - ribosomal large subunit binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="60S ribosomal export protein NMD3"
                     /protein_id="HNQ29422.1"
                     /translation="MHIRESICPLCGNPSPSGEVCGQCRALSTRWLECPPRVSTVLCP
                     TCGARKDAGGWSDLQLEREELARQLIKRAIWLHPDLRDVSMNIALDEISTNRSRAECR
                     ISGILFGAPVEGSCSLEVELRREQCDRCSRISGSYYEGVVQVRATGRRPSQRELEETA
                     RIACAVEASMTESGERLSFISRLEEHRDGIDIIVGSQRLGSEIATAISRDLGGRFTTH
                     PKLVGEKAGRQVFRITYSLRLPRYSRGDIVLVSGRHLEVLGVDGKNIRCLDHASGMVR
                     SVPERQVERKAGHKGDALEWMVAYRDGDLLGVLDPRTGVTKEVRIPRGKETSAGTTVR
                     ILEDGDLLVLLE"
     gene            19869..20738
                     /locus_tag="PKM87_04810"
     CDS             19869..20738
                     /locus_tag="PKM87_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNQ29423.1"
                     /translation="MRVREIPVNELCSLKGADWVDRNRRTYLDGNIAFVPVREGCPCT
                     STLPQRRPYQGRGYFMLGDVAVFHGRRPGEDEVRNLMEWKRPAGVLWVQSCKGTMRTP
                     QTEVLAGTVQEVRHRECGITYWLDPASIMFSMGNRDEKERIRSLVHPGERIADMYAGI
                     GYFTLPSALAGARVHAMEIRPLAFSYLERNIIGNDVAHLVTAELGDCRDCLLGTYDRI
                     IMGHFGSIDRLTDALAHSGPGTVLHVHSAGALKDKIQAILEDAGVTAAIAIHKVKKLA
                     PHTWHYVQDVTLE"
     gene            20735..21907
                     /gene="coaBC"
                     /locus_tag="PKM87_04815"
     CDS             20735..21907
                     /gene="coaBC"
                     /locus_tag="PKM87_04815"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNQ29424.1"
                     /translation="MSGINTLEGKEIVLAVTGSIAAVDTVRLAHALRRRGARVQAVMS
                     SAACGILHPAALTYATGRPAITGITGMVEHVAWCGEGGMADLLLVAPATANTLCKIAA
                     GIDDTPVTTFATTAVGRGMPIVVAPAMHESMFRHPGVAGCLRTLEGWGITVVGPRIEE
                     GKAKIADIGDIVLHCERELMGKPLLGHHVLVTSGPCRERVDDVRVLTTRSTGLMGRAL
                     ALQAFRLGARVTVVHADRFPAVENVRTTDAAGMLKEVTRICREDPVDLFISAAAVSDF
                     CPEPCPGKIPSGNTVTLTLNPLPKIIDDVMDRFHVPTIAFKVGDDAVTRGRTMLGDSI
                     GLVVANDPNVMGEVDTRVTMLGQGGFVREASGSKEAVAAQILDCAMEIFRFPKKGR"
BASE COUNT         4729 a         6870 c         6445 g         4573 t
ORIGIN      
        1 atcaccaggc gggagcggag gctcgggcac tcggtgcaga tctcctcgac tttggctgcg
       61 ttctcgttgt ccgtgattac catccggatc ttggccgcgt tgatccggta tttcaggtcc
      121 tttccggtca gcatcgtggg gcaggggcag aagaccgccc cgagcttgat gcaggcgagc
      181 gcgaatatcc accactccgg gaccctcgtc agcatgacaa tcacccggtc gcccttcttg
      241 atgccgtact tgaggagcat gttcgccgcg gagttggaga ggttcatcat ctgcctgaag
      301 gtgaacgtct tctcgtgtcc ctcctggtcg acccagatca tcgcgagccg gttcctgtcc
      361 tttgcggcga gacggtcgac ctggtcgaac ccgaagttgt agtattcagg gaccttgagg
      421 gagaagtgat catagacttc ccggtaattg tgcatgttgt gctccgctgc agccatatga
      481 actgatatgt tgtgcgagaa ccgacaaaaa gatgctcttt tttctcgcat caggcattca
      541 aaatcaggaa tttgcagggg agaaagatta tccggagaaa atgaggtcaa aaaagatggg
      601 aattaccggg agctgttgaa cctctcaagc tcccgtaccc gaagctcgcc ccgcctgatc
      661 tttccggaga gtgtctttgg gagatcgtgt gtgaactcga tcgcccttgg gtacttgtag
      721 ggagcagtgt tcttccgcac gtgggtctgg atgtccttca ccaggcgctc ggagggttcg
      781 aacccgggtt ttaagaccac gaatgccttc acgatggtcc cgcgtatgag atcgggcgat
      841 ccgaccaccg ccgactcagc gaccgccggg tgctcgagca gtgcagactc cacctcaaag
      901 gggccgatgc ggtagccgga actcttgatg atgtcgtcgt cgcggccgac aaaccagtaa
      961 tacccctcgt cgtccatgta ggccttgtcg ccggtgtagt aaaacccgtt cacgaacgac
     1021 tctgcgtttg cctcggggtt gttgaggtac tcgacgaaga ggcccacggg tcgcgggtcg
     1081 agcttcactg cgatccttcc ttcctcgcct ttcggaacgc ggttcccctc ctcgtcgtgg
     1141 agctcgatgt gccagccggg agcgggcctg cccatcgagc cgggcttgac cttcatcccc
     1201 cggaaggtgc cgatgcagca gaccgtctcg gtctggccgt acccctcggc gatgggaagg
     1261 ccggtcccct cctgccagac ccggaccacc tcggggttca ggggctcgcc ggcactcacg
     1321 cagcgccgga gttcgcggag gtcaaacttc tcgaggtcgg cgaggatcag catccggtag
     1381 atggtcgggg ggacgcagaa ggtggtgagc tcgtacttct ccatcagcgg gagaagctcg
     1441 gttgccttga acttgccccg gacgtcgtag acaaagaggc atgctccctg gatccactgc
     1501 ccgaagatct tgccccaggc gcatttcgcc cagccggtgt ccgagtaggt gaagtggaga
     1561 tcgttttggg taaggtcctg ccatagcccc gcagtgacaa ggtggccaag ggggtaggcg
     1621 tggttatgga ggaccatctt cggctcacct gttgtccctg aggtgaagta gatgagcatc
     1681 gggtcggttg cgagggtctt cttgtcgacc ggcatgctca ccgagtggtg cgagaccggc
     1741 gcagggaaga agagctcgtg ggggtagctg atccacccgg gacgctcgcc atccaagagg
     1801 aaccggcagg agagggaggg acactcctcg gcgatttcgt cgactttccc tgcatgctcg
     1861 aggcttgtga tgaccatctt gaacttcccc gcgttcaggc ggtacctgat gtcctttggg
     1921 gtgagcatgg tcgggcaggg gcagaccacc gccccgagct tgatcagtgc gatcacgaat
     1981 acccaccact gggggaccct cggcaggaca aggagcaccc ggtctccctt ctggatgccg
     2041 tatttgatca ggatgtttgc cgcctggttg gagtggttct tcatgtcgcg gaacgagaac
     2101 ttcttctcct ccttctcctg gttgacccag atcattgcca gcttgttccg gtccttctcg
     2161 gcccatgaat cgatgacgtc aaagccaaaa ttgaaatatt ttgggacgtg gatctcaaag
     2221 ttcctgcaga gatcctcgta acacccctgt gattcggttg ttccctccat aaaaacctat
     2281 attatttctc aattcccgga tttgaatctg tccatttttt ccgggaaaaa gtccccaagt
     2341 tgtgacataa aggtatccga ttagtattat gctgcgggaa aggggatgtt attagcaatg
     2401 gtcggttcga ggtacgaatc cttaaagatc gagaatatcg tggcctccgg agccattgcc
     2461 gatgcgatcg acctcgcaga cgtatcgaag aagatcgaac actgcgagct taaccagaaa
     2521 cggttcccag gcgcggtatt ccggatggaa aacccaaaga tcgcgtctct catcttctct
     2581 tcaggcaagg tggtgctcac cggtatccgc gaccaggact cgcttgacaa agggctctcc
     2641 atgataatcg cggcaatgaa agaggcgggg gtggatacgt acccggagcc gcgggtgggc
     2701 gtcacgaata tcgtctgctc ctacgatatc ggcaagaata tcaacttaaa caaggtcgtt
     2761 atcaccctga acctcgagaa tatcgagtac gaacccgagc agttcccggg gctcgtatac
     2821 cggatcaagg acccgaagat cgtggcactc ctcttctcgt ccggaaaaat aatcctgacc
     2881 gggggaaaga ccctcgatga gatcaggcgg ggcctcgact tcctcgagca gaagatcggc
     2941 gggatcctct gatcaccaga accgcctggt gcggatgtag tttcgttccg ccttgatgat
     3001 ctcacggtaa aacccatccc cctccgcgag ggtggagagg attcgcgagg cattctcggg
     3061 gccgactccc cttgcggcaa gcgcggtcac cgcctttttt ccccccgaga gcacgatgtt
     3121 tgcattccgg aggagccgtt gttcagtcgc ccgctcctcc tcgttctgcg tctttttctt
     3181 ctttgccagg gcaacaagcg gctcctccca tggctttaat accgcaacaa gccctgcacc
     3241 gcagagcggg cagcgaaggg gatctgggac tctcgcgacc gtcgtcctgc tcttccactt
     3301 cctgcagtgc atgcaggcaa ggaccacctc ctgctgctcc agtctgcgct tgagggttcc
     3361 gatcaccgcc tggtccgcgg tgggcgggga gatctggtcc ctggatgcga acagcccttc
     3421 tgccccgaga atgctcaatg gcgagactgc cacatctatg gacccatccc tgatcagcga
     3481 gacgatctgt gccgcggccg ggacatccat gtacaccgag aaaagctcgc ggtaggcctc
     3541 cttctgcacc accgttccat caaagatctc gacgagccgg tgggtgctga ttcgctcgta
     3601 atctgcatcc gggtcgatcg ccccgaactt ctttgcgatc tgtaccatct tccacttgaa
     3661 gagtgccgtc cgtttcatgg caagcttcag gatgccctcg aggtgaatcg ggtcgagaga
     3721 gagtagtatc tcccgtacgt ctgccgcctt caccgccgaa gggaggcgaa ggtagatccg
     3781 ataggcatcg atctcgatcc ccacggtggt cccgtacctt gccgagagga ggatggagag
     3841 gacgcgggca agggcctcgt ttgccttgtg tccggcacag acgttgcaga cgaccccctc
     3901 gggggcgttc tcgagggtga tcagggtgtc atcggggacc cttgatctgt tcctgtccat
     3961 ctccacgagg aacctatgtg caaacccgag cggttcttct ctggcatggt agggagaaaa
     4021 gtcccttgac ctcctgagcg cccccacttc acgggcgaca gagaacggga cagggatctg
     4081 ctccccctcc cacgagggga gttcgccctt tgccttcttt gccggctcga cagtgacctt
     4141 cccctcctcc atgtcaagta cccgccagag ctgccccttg gtgatgaaga ctgccccggt
     4201 gtgcaggaac ccgacgacga acgactcgtc gagcgtcccg acggtcttcc ttgagaccat
     4261 gtcaaagacg acaacctttc gttcgtcgtg gatcatcgag aggttcgacg agaggtaccg
     4321 ccgcgatctt gccccggacc ttaccatgtc accctcgatg cgaatgaggc cgtgctcggc
     4381 catctgggtg cagacctcgt caacgaggtt tccggccatg gcaaacgggt agctcctctc
     4441 cacgatgtcc ctgatcttct ggcgggagat ctcccggtac tcaaccgcca gcgctgctac
     4501 ctggtttgca aggacatccg cggcattgag gtgaaggctc acgtcttcaa cctgggctga
     4561 tttcgccctt ctggcgatga cgagggactc gagcaggtca tcaaaaccgg tcgcaagcac
     4621 tgttccccgg gagaccgcgt ccatctggtg accggcgcgt cccacacgct gcacgagtcg
     4681 tgcgacctcc cgcggagacc caaactggat cacatgctct atccggccga tatcgatccc
     4741 aagctccatc gatgatgtgg agatcagggc acgcaccgca ccggacctga accgctcctc
     4801 tgcctcaatc cgcacctcct tcgagagcga tccgtggtgc acctcaacgt cgccgcgatg
     4861 atagaggtga tggccgagtg cctcggcggt tacacgggta ttcacgaata ccagtgtgga
     4921 ctcgtgccgt ccgatggccc gggagaccca tgcggcctgc ttctcgaaag agtccccggc
     4981 aaaatcgacg gatatctcga ggtgctttgc gaccgggaca gagacgatgg aaaaatcacg
     5041 tgccccacag aggaaccggc ccacgtcctc cgggtttccc accgtggccg agagccctat
     5101 ccgctggaac tcccctgcat actccttcag ccgctcgagt gccaccgcga gctgggcacc
     5161 ccgcttgctc cccgcaagct catggatctc gtcgaccacc acgtaccgta catcggaaag
     5221 atgttttcgc agcaccttgc ccatgaagag cgcttggagg gtctcagggg tagtgatcag
     5281 gagatcgggg gggtggagcg cctgtttcct gcgctctgat tgcggcgtgt caccatgcct
     5341 gaccccaacc gagagcccaa gttccctgca ccaccattcc aggcgctgca acatgtcccg
     5401 gttcagcgac cgcagggggg tgatatagag cgtgcagaac ccgctcccgg tatgggacat
     5461 catcgcatcg aagaccggga tcatggcgct ttcagtcttg cccgtgccgg tgggggcaat
     5521 caggagaagg tggcggccct ccctgaccag gggaatggca ttctcctgga tctcggaaag
     5581 gcgggaaaag ccccgggacc ggatgcactc ctgcaccctc cggtcaagcg tccggagtat
     5641 ctccatggtc ctccagggac gaaagcggcc cgaggaacgt cccgtctgcc aggaacacct
     5701 ccgactgttc ggcctttatg cagcgggaga gggggctgaa gggttccctt accgtgcgca
     5761 tgatgtcgta ccctgcaagc tcgttcaacg cggggatgaa gagcacccgg gtctcttccc
     5821 cctgggtatc agcagcccgg gcaaactgca ggcatgcatc atccagcggg gccagcaggt
     5881 atcccggtgc cctgagcgca caccccactt cgtcgcggat ggacaccata ggatggtggt
     5941 gaccggcaat gatgagatgg ccctgcaagg agggatcggg gcaggtatgc ccatggatat
     6001 atcccacccc atcgatcact gcaccggttt ttgggagcac ctcgtcatcg tggaagaact
     6061 tctcgattcc cggatcatgg ttccccggca gcagacggag cggaaccttc gaccggaatg
     6121 cgtccaggat agcggggagt tcccggtact cctgcctgct tgtgagggga acattgtgct
     6181 tcacgtcacc aagaaggacg aagagttccg ggtcggctgc ttcaatgcag gcgataagcc
     6241 gctccttcct ctccctgctc cggctcctga tgtggaggcc atgccgcgag agatcggact
     6301 cgatgccaag atggaggtct cccgcgacga ggacacgcac atccccctcc acaaggagtg
     6361 caggacctgc gttcaggaac tcaagcctca taacactttc aggacgcccc gtgcgggctg
     6421 gtagcattcg tcctcgagca gtagcgattc cacggcctgt ttgacctcgg acgcggccag
     6481 cccgtgcttt gcagccttac gggttacctc ctcgagggaa atcccagtct ttccgccatg
     6541 ctccctgatt atttcaagga cgagtgccac gggatcccct gcttccggag aggaagcgtg
     6601 tggggcattc actcccttaa tcgcctcttt caccatgagg gccaggtctc tcagccgcga
     6661 ggcgtcaaga tggtagtgca agacaagggc atcaagccgg gcatccctcc tcccttcgtc
     6721 aagtgctgtg cggaggtcct caatgcgccg catggtaaga tccgcggtgc gcaataccca
     6781 tgcatccctt acataccggt caacttcccg catctcgatg accctgactg tgcacctccc
     6841 cgccccccct ttccccgtga tacgtgcctc accaatgacg gttgcaaagc acgggatctc
     6901 catcgactca aggatatctg ccgtgacctg gtcggtgcga tcgatgtgga gttcgaatgc
     6961 agcggtcgaa tcggcgaggc gtgcatgcat gaactcccct ttccggccct gccgctccag
     7021 gacggctccc gcacagaaga gggtggtgca gatcgctcct cccggcgtga ggactgcaac
     7081 gggagagtgg gttccactct ccgtaaatgt cgcgatggac ccattatact ccctggcaaa
     7141 caccctctgg taagttccgt tcatgtaatt ttacactctg gaatgtggca catggctcag
     7201 cctctccaat cctgcctgaa ctatgtacga gtggaatgca gatagccatt ctgataccgg
     7261 aatcaacctg cctttcagcg atcagctgcc agcaaccaac tgaaatattt ttcgcatcaa
     7321 accttgccgg taattattgg cagattgaaa aaatacgtcc ttttcaccaa atttttacca
     7381 ctacatttat atattgtaac ttactccaga tcacagtaca gggtttttca tgaggacaga
     7441 aaggctaagg tggctagtca tacttgtctc tctcttcatt acagttgtat ccggtacgat
     7501 cggtttcccc ctggttgacc tgcacatcgg tgatgcaggt ttcggaaatg aactgccggg
     7561 tattgccccc tccgtctttg acgggggaga gagagggctg caggaaaggg taacaacaat
     7621 gcccctgctc ttcatcgaga atgcagggca gagcgatgat gaagtcgagt tcgtggtcat
     7681 ctcggacggg ggatccatct tcttcaccag ggaaggcgtg acggtcaaac tggtgacatt
     7741 gatggaggat acccctgccg cgacattggt tggatacagg ttcgtcggtg cggaaccatc
     7801 tccccggatc acaggtcttg acccccttga gggaaaagta aatttccttg tcgggaatga
     7861 gcctgatggg tggcgaacgg gcgtcccgac atggcgcgca atacggtacc atgaccttta
     7921 caggggtgtc gatctcgtgt acgagggaac tcctgaaggc ctgaagagtg cattcaccgt
     7981 ggcacccggt gcgtccccgg gggatatcac actcgaatat tcgggcattc tcggcctggt
     8041 ccttgcagag gatggatcac tgcatataaa aacatccgcc ggggaactcg ttgagtccgc
     8101 tcctgtgtgt taccaggaga ttgacgggga ggtaatacct gtcggtgcct gttacgtgct
     8161 gaaaggaagc agcagggtgg gctttatgat tgggccgtat gaccagacct gcccccttgt
     8221 catcgacccg gtgatgaaat atgggctcta cctgcggggg attggagttg catacgggag
     8281 atctgttgcc ctggatgata caggaaatgc ctacctgacc ggcgagacct ttcccgcacc
     8341 ctacaccatg agtccgggct cgaatgacat gagcggtgat ggaaccgacg tggtggtcgc
     8401 aaagattaac ccggacggaa ccgttcctct ctacgtcacc tatatcgggg gagatggcga
     8461 tgatatcggc cggggaatac gcgtcgacgc cgaagggaat gcatatgtca ccgggcagac
     8521 cgcctcagcc gattatcccg tcgtgaatgc ggtcaagtcc accctgtcgg gccccagtga
     8581 cgcgttcgct acccgccttt cacaggatgg tgcaacgctt gtcttctcga cataccttgg
     8641 cggggcacag gatgatgccg gaaatgccgt ggccctggat agtgcaggag atatctatat
     8701 cgcgggcaat acgatgtcct cggacttccc gggaatcagg gtccctcaca acaccaacta
     8761 tggcggaaac caggacgcgt ttatcctgaa gatgagcggt gatggatcgc aggtggcctt
     8821 ctttgagttc atcggcggga gtaaatcgga ttcagggtat ggtgttgccg tagattctga
     8881 gggaaactcc tacgtcaccg gcgagacgtt ctcactcaac ttccccgtga tcaatgcgat
     8941 gcagggttcc cttgccgggg ggaggctgag cgacgcattc atcaccaagg tcagccctga
     9001 cgggagttcg ttcgtctatt caacctatct cggcggatcc caggtggact cggcccggtc
     9061 catcggggtt gacgcaaaag gtcgtgcaca cgtgaccggg gcgaccattc tcggagtatt
     9121 ccctgtgaaa gacgcattcc agccggtggc aggcggctgc actgacgcat tctacagcag
     9181 gctctctcct gacggcagct cgcttgagta ttcgacctat atcgggggag taaatattga
     9241 cgaaggaaaa ggtgtcgcag tcgacgaatg cggggtcgca tatgtgacgg ggctcacctt
     9301 ctctcctgac ctcccggtca tcaatcccta ccagcgtttc cttggagggg aatcaccgtt
     9361 cagtgttgag gtatctgatg cctatgtcgc gaaattcgtt ccatgggaca cgatacccga
     9421 gtatgtcacc tatctcgggg gagacggtga cgacgtggga gcagcgattg caatcaaccg
     9481 ccaggcatgt gtcgattgca ttgccgaacg cacgtgtgct tccattaccg ggtataccga
     9541 ttctaccaat ttccctgtga gcgatcccta ccccatctcg ttccagccaa agggggaggg
     9601 aggatttctc tttaccctgt gcgacgatgg cgaatcgcct ggaatgccgg ttgctgattt
     9661 cactgcagag cctacttctg gttgcccggc gcctctcctc gtccagttca ccgacctttc
     9721 cacggggata cccacctcct ggtactggga attcggtgac ggtgcgacct cgaccctcca
     9781 gcaccccacc cacacctaca ctacacccgg cacctttaat gtcaccctca ccgtgaccaa
     9841 tgtctgcggg tctgactctc tcacaaagta cgagtacatc tcgatatgcc ccatctatgt
     9901 cccggtagca aacttcactg cagagccaac ttcaggctgc ccggcgcctc tcctcgtcca
     9961 gttcaccgat acctccctcc atgatcccac atcctggaac tggtccttcg gtgacggatc
    10021 gttctccact ctccagcatc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 acgtcaccct caccgtgaca aacgaagccg gctcagactc actcaccaag cacgagtata
    10201 tctacgtctg cccgttcatt cccccggttg ctgacttcac tgcagagcct acttcgggat
    10261 gcccggcgcc tctcctcgtc cagttcaccg atacctccct caataacccc acatcctgga
    10321 actggtcctt cggtgacgga tcgttctcca ctctccagca tcctaaccac acctacacca
    10381 caccgggaac ctataacgtc accctcaccg tgacaaacga agccggctca gactcactca
    10441 ccaagcacga atacatctac gtatgcccgt tcattccccc catcgcgaac ttcacgtata
    10501 tctcggaatg cccggactcc gaattcgtcc ggttcaccga tacctccctc catgatccca
    10561 catcctggaa ctggtccttc ggtgatggtg agacctcctc tctccagcac cccaaccaca
    10621 cctatgccga gacaggggga tacaacgtca cccttaccgt gacaaacgag gctggctccg
    10681 actccaccac ccagatcatc tgcgtggttc catgcatcga tcctcctgtc gccaacttca
    10741 cgtatatctc ggaatgcccg gactccgaat tcgtccggtt caccgatacc tccctccatg
    10801 atcccacatc ctggaactgg tccttcggcg acggatcatt ctccaatctt cagagtccaa
    10861 cccacaacta cgctgagaca ggcggttaca acgtcaccct taccgtgaca aacgaggctg
    10921 gctccgactc caccacccaa atcatctgcg tggttccatg catcgatcct cctgtcgcca
    10981 acttcacgta tatctcggaa tgcccggact ccgaattcgt ccggttcacc gatacctccc
    11041 tccatgatcc cacatcctgg aactggtcct tcggcgacgg atcattctcc aatcttcaga
    11101 gtccaaccca caactacgct gagacaggcg gttacaacgt caccctcacc gtaacgaacg
    11161 acgcaggctc cgactccacc tcccagatca tctgcatccc cgagtgtccg gggccggtgg
    11221 acccgccagt ggcagatttc acctatgtct cggaatgccc ggactccgaa ttcgtccggt
    11281 tcaccgatac ctcactcaac aaccccacct cgtggttctg gaccttcgga gatggtgaga
    11341 cctccgacga gcagaacccg acccacacgt actcttcaac tggcacctat aacgtcaccc
    11401 ttgcagtctc caattccggt gggtccgatt ctatcacaaa gaacgtgtat gtgccctact
    11461 gtccaggacc cgtggatccc ccggtcgcga acttcacgtt ctgcagtaat ccctccgatc
    11521 cgctcaccgt atacttcacc gacacctccc tcaatgaccc cacttcctgg ttctggcaat
    11581 tcggcgacgg aacaacctca aatctccaga accccagtca tacattccct gcagtcagcg
    11641 gctatgaggt caccctgacc gtaacaaacg aaggtggaac ggattccatt tccaagattt
    11701 tatgcatcgc ctgcataccc ccgtgccctg aaccacccgt cgcggatttc acctatatct
    11761 cggagtgccc ggactcccag ttcgtccggt tcaccgacac ctctattggt aaccctgctg
    11821 catggttctg gcagttcggc gatggggcta cctccactct ccagcacccc aaccacacct
    11881 acgcccatac gggcggatac aacgtcaccc tgacggtctc aaacaaggga ggttcagact
    11941 caatcaccag gatcatctgc atccccgaat gcccaccgcc gaatccgccc atcgcgtgct
    12001 tctacgcaag cccccgcacc gggtatgcac cgctggaagt ggaattctgc gactgctcgg
    12061 agactgatat cctcacctgg agctgggact tcggtgacgg cggcacatcg acggagcaga
    12121 ccccgcggca tatttaccag gaatacgggt cgtacacagt gagcctctat gtgaccaatg
    12181 caaccgggta tgataccgag acgaaggcag actatatccg cgtatcttct cctctgcccg
    12241 gaccgccttc cgcgtatttc accgcgaacc agacaacggg gcacgtgcct ttcgccgtcg
    12301 cattcactga cgggtctacc ggggttcccg agacctggtc atggaatttc ggcgacggag
    12361 gcacctcaac tgatcgaaac ccagtccacg tgtatacgat gccaggttgg tattccgtca
    12421 gcctgacggt cgagaattca tatggttcct cgacttactc aaggcctgag tacatcacgg
    12481 cccaagacag cacgggcggc gacaaaggtt acttcctcgt tcactgcaac gtggatggtg
    12541 cagcagtcac gttcaacgat gattacaagg ggctcacaga ggatggaacg ctgcttgtca
    12601 tggtctacgt gaccgggaca cactataccc actacacggt tgggaaggat ggctattacc
    12661 cggtgacgct gccaatctcc acttatcctg cgaaagacca gacactgcac ctgtatgcca
    12721 ccctcgagcc ggtccccggt gattatacta tcactgcgac tgcaggtccg aacggggcca
    12781 tcatcccgaa cggcaccttc acggttccgg ccggttctga ccgcgagttt accgtaatcc
    12841 ccaacccagg ctacaaggtg gagaaccttc gccttgacgg gcaggggctg ggagcgctca
    12901 cctcctacac gcttgaggat atcagcgaca atcacaccct ggccgccact tttgtccaga
    12961 atgagaccgt attgcacgtc atctctgcca ctgcagggcc ccatgggcag atcgttcctt
    13021 cgggccagat cctcgttccg gacggtgcaa ctgcagggtt tgcaatcact ccccacctgg
    13081 gatacagtat ctccgatgtg cgggtgaaca atatctcagt cgggatgccc tcgtcgtacc
    13141 agttcatcaa tgtgacccac gacaacacga tcgttgccga gtttgggaag acgaatttca
    13201 gtatttccgc acttcccacg gagaacggga ccatcatccc ctccggtgtg accgaagtgg
    13261 cctacctgga gagtttcacg tatgaaattg tacctaattc agggttcttc accgcgcagc
    13321 tctttatcga cggggaaagt gtgactccgg cccccaccta cacgttcgac aacgttgccg
    13381 caaaccatac catctccgca gtcttcctgc cggacgggtc cctgaccttc tacatccagg
    13441 caactgcctt tacggggggg aacatcattc cctcagggaa cgtgagtgtc cttggaggaa
    13501 acgacatcac gttcaccata tatcctgact ccggccatgt cgttcagaac gtcatggtgg
    13561 acggtgacag caagggcggg ctccttgact ggacattcag ggacgttgcg gcgaaccata
    13621 ccatcagtgc aaccttcgca ccctccggtg gcggcggcgg aggcggtggt ggcggtggcg
    13681 gtggcggcta ctccgtgagc acgacaaccc agcccaccac ggtccccact acgccggtcg
    13741 ccgagaccga gggatacgag agcggtacaa gtgaagagac gatcaccccc actcctacag
    13801 ccactgccga ggtgaccggg gttgagacga ttgtcaccac ggtacctacc acgattcccc
    13861 ctgccgaacc tttctggacc agattcccgc tctactggct cgtgccactg atcattgtca
    13921 tcctcatcct ggccgctgtt gcatactact actggaagaa gaaacagcag caggaagtcc
    13981 tgttcgaaga ataacgattt gaattctctt tttttaccct ctcatagaca gcgtgccagg
    14041 gcgtctccgg gctttcccag caggattgtt acaccatgca cttcgacatg gcatcccccc
    14101 aatcacaaca catttcttct cctgcccccg atatagtgga ggtaagattt gcggactttt
    14161 tacaggggtg gagaagcgcg atgaacgata ccacgatgat atggatagag aagtaccgtc
    14221 cgcaaaccct tgcggatatc gtgggccagg acgagatcgt cgagcgcctg cagtcctatg
    14281 tgaagaccgg gaacctgccg cacctcctct tcaccggaag tgcgggggtg ggcaagacca
    14341 ccgcggcagt cgcactggcc cgggaattct tcggggagtc ctggaacctg aacttcaggg
    14401 aactgaacgc gtcagacgag cggggcatag acgtcgtacg gaaccagatc aaacagttcg
    14461 cccggacttc tccgcttggc ggtgcaactt tcaagatact cttcctggac gaggccgacg
    14521 ccctcacccc cgacgcccag gcggcgcttc ggcgaaccat ggagagctac gccaccacct
    14581 gcaggttcat cctctcctgc aactactcct cgaagatcat cgatcccata caaagccggt
    14641 gcgcaatcta ccggttcagg agcctctcgg gtgaggccat cacaaaggag atactgcgaa
    14701 tcgcagagaa cgagaagatc agcatcacgg agcccgcaat ccaggcgatc gtatacatcg
    14761 cccaggggga catgcggaaa gccatcaatg cactccaggg cgcagccatc cttgcggccg
    14821 agatcgacgc cgggatggtc tatgccatca ccgcaacggc acgccccgac gagatcgagg
    14881 atctgctggc cacgtcactc tccggtgact ttgaaggggc ggaggcgatc ctccaccatc
    14941 ttctccagga ccggggaatt gccccaaacg aactgatcaa ccagtgctac aggaccatcg
    15001 tgaaaaggga catggacccc gaactccggg tagccctcat cgaccagctt ggcaccacgg
    15061 atttccgcct ctctgaagga gcgggcacag agatccagat ggaagccatg atcgcccagt
    15121 tcgtgctcca ggcaaagaag catggctaaa cgagcagatt ccccggccgc cgaggccgcg
    15181 tccgagatcg tcgatcgcga tgccgactgg agcaggttct taaaaagccg ctataaaaag
    15241 caactctcgg aacttgcccg ggagtatcct taccggcgct ccctccagat cgattaccgc
    15301 gagctggaga gctatggccg tgcgggcctc cgcatggccg atgagctcct ggacaacccc
    15361 ggaaaggtca tcgaggacgt gcgaaacgcc ataaagaacc accagctcgt gaaggggagg
    15421 gacgggaagc ctaccggcga ggtgaatgtc cggttcgtga accttccccg caagatcggg
    15481 atccggcaca tccgttcgga ccacatcaac cggttcatca gcgtggaggg gatcctcagg
    15541 aagacgaccg aggtccgccc ccgcatcgtg caggcggtct tcaggtgccc gggagggcac
    15601 atcactatca aggagcaggg gtatggcaag ttcagggagc ctgacgggtg cgcgacagag
    15661 ggatgcacct tcaagaagct tgatcttatc gcaagacggt cacgtttcgt ggattcgcag
    15721 aaactccgaa tccaggagtc cccagagggt ctcagaggtg gcgagcagcc ccagaccctt
    15781 gacgtggaca tgaccgacga cctgacagga atggtcgcac cgggcgaccg ggtggtcatc
    15841 aacgggattc tccgatcaat gcagcgggtc acccacggtg agaagaacac cctctttgac
    15901 atatacctcg aatgcaactc catcgagatt gccgaaaaag agttcgagga ggtgcagatc
    15961 gacgaaaaag acgaggagga gatcctcacc ctgtcccgcg atccaaacat ctaccggaag
    16021 atcatccact ccatcgctcc caccatctat gggaacgagg acgtgaaaga ggcaattgca
    16081 ctccagatct tcgggggtat aagcaaggag atgcccgacg gaagccacct ccgcggagac
    16141 atccacgttc tcctcatcgg ggacccgggg attgcaaaga gccagctcct ccggtatgtc
    16201 gtcaagctct ctcccagggc aatttacacg agcggccagt cctccacgtc ggctggcctt
    16261 actgcgaccg cggtaaagga cgagttcggg gacgggagat ggacgcttga ggccggggcc
    16321 ctggtgcttg cggacatggg aatcgcggcc gtggacgaga tggacaagat gcagaaggag
    16381 gaccggtccg cgctgcacga ggccatggag cagcagtcca tcagcgtcgc gaaggccggg
    16441 atcaccgcca ccctcaagtc gcgctgtgcg cttctcggcg ccgcgaaccc aaaacttggc
    16501 cggttcgacg cgtacctccc catccaggac cagatcaaca tgcccggctc cctcctctcc
    16561 aggtttgacc tcatcttcaa gatgaccgac atccccgacc acacgaggga cagcgcgatt
    16621 gccatacaca tcctcaaggc tcacagcatc ggggagacca tcgcccagca caaaaggact
    16681 ccaattcccg gggtggacga cgcgtatatc gaggagcagc tccggcctgt gacccctgag
    16741 atagaaccgg cactcttcag gaaatacgtg gcatatgccc gccggacatg cttcccgcgc
    16801 ctcggtgatg ctgccaggga ggcgctcatc ggctactaca tgcagctgcg tgacctcgcg
    16861 gcaggctccg ataagcccgt gccggtcact gcgcgccagc ttgaggcgct ggtccgcctc
    16921 gcggaagcga gcgcacggat ccggctctcc cccaacatcg aggctgaaga ttcggaacgt
    16981 gtcatcaaga tcgtggacac gtgcctgcgt gaagtggcct acgatgccaa gaccggcacg
    17041 tttgacatcg acaaggtggt gacgggaatc tcgaagggaa ggagggacct gatcagagtg
    17101 atcaagcaga cgatacgcca gatacagaaa gacaccggga atgaccggat cgcagttgcc
    17161 gaactgattg acctcctcac cccgcaggga ttctcccggg atgagatcga tgcccagatc
    17221 aaggtgtttc tccgcgaagg cgaggcgatg gagccgaaga gcggctttgt caagcttatc
    17281 tgaaccccct tacttttttg cttcccggcc gagaactaca tccatgcaca ctgcaaggga
    17341 aaaagcagct tttgaggccg ggatcaaact cggggccctg taccaccagt gggtaggaac
    17401 ccctgtttca cccctcacgg ccgcatccat cgaatccgcg atagaaaaga gtgtcgggct
    17461 ccagcctcat gtcaagggca tctccgtcag gctcgaccgg gatatcatga agccgaacgt
    17521 gttcgggtac tccgagctgc aggggctcat gctcacggtc gagatcgaga cccgcgttga
    17581 cggcgaggtc tgccgtgccc gcctccggcg cgagggtgac tacccaatga tggagatcgt
    17641 ggagtagatg ggcggcccgc ggttcccgat cacggaccat cacatccacc tcgatccccg
    17701 gaacgggcgg ggtatcgatg ctgcaaagga cttccggcgt tcgggtggaa cgcatctctt
    17761 cctggtcagc aagccctcct cctcctttgg catcctgccc gtgaagggag aggacttcct
    17821 cccggtcttt gaggagaccc tggcggtcgc gcggatggtc tcagagactg gcatcacggt
    17881 ctaccccgtt ctcggcgttc atcccgccga gatcacgcgc ctgatggaat cgtttccact
    17941 gtcccgcgtg gtggagataa tgaaggaggg tattgcactt gcggccgggt tcgtcattga
    18001 cggcgcggcg gtcggcttaa aaagcggaag acctcactac gaggtctccc ctgaggtctg
    18061 ggatgcggca aacgacgtcc tctcatactc cttccagaag gccggcgagg ccgggtgcgc
    18121 gatccaggtg catgccgaga gcggtccctg tgccgacatg agggagatgg cgcgggctgt
    18181 cgggctctcc cccgatcagg tggtaaagca ctacgcaatc cccgaaacaa cccttgttcc
    18241 ttcgctgctt gccacccatc ccgatatccc ggcactcgcg atggaggggc gggcgttcat
    18301 gatggagagc gattatatgg acgagctctc ccgtcccggg gcggttatcg ggccacgctc
    18361 ggtcccgcgt gtcaccacca ggcttctctc gaccgggcag atcgatgagg atgcaacgca
    18421 ccggatccac gtatccacgc catcgcaggt ctatggcgtg gatatctcgc tctgatgacc
    18481 tccagttgca gtattaatac gcatccctcc ctacatgtgg tatcatgtat ctgaaggttc
    18541 accgctctcc tgccggggac gaggtggtgg cagtatgcga ccgggagttg atgaatacca
    18601 cgctctctgg aaacgaagta gagattcgca tcaccgggtc attttatggg acagaacttg
    18661 caaccccgga agaggtgagt tcggcactct cccgcgccga gaacgcaaac ctcatgggtg
    18721 agcgaaccgt ggccctggcg gtggagatgg ggctcctctc ccgctcggcg tgcctgatgt
    18781 tcggtgatgt gccccacgcg caattgttca ggatttaaaa atatgcatat cagggagagc
    18841 atctgccccc tctgcggaaa tccctcaccc agcggagagg tctgcgggca gtgcagggct
    18901 ttgagtaccc gctggctgga atgccccccg cgggtatcca cggtgctctg ccctacctgc
    18961 ggagcgagga aagacgcagg aggatggtct gatctccaac tcgagaggga ggaacttgcc
    19021 cgccagctta tcaagagggc aatatggctc catcctgacc tccgcgatgt gtcgatgaac
    19081 atcgcactcg acgaaataag caccaaccgc agcagggccg aatgcaggat ctccgggata
    19141 ctctttggtg cgccggtcga ggggtcctgc tcacttgagg tggaattgag gcgggagcag
    19201 tgcgaccgct gcagcaggat ctcggggagt tactacgagg gagtggtaca ggtacgggct
    19261 acgggaagga gaccttccca gagggaactg gaagagaccg cccggatcgc ctgcgcggtc
    19321 gaggcgtcga tgacggaatc cggggaacgc ctctcgttca tctcccgcct cgaggagcac
    19381 agggatggca tcgatatcat tgtcggctcc cagcggctgg gctccgagat agccactgca
    19441 atctcccggg accttggcgg caggttcacc acccacccaa agctcgtggg ggagaaggcg
    19501 ggcaggcagg tcttccggat cacctactcc ctccggcttc cccgctattc ccggggcgat
    19561 attgtcctgg tctctggcag gcacctcgag gtgctcgggg tggacggaaa gaacatccgg
    19621 tgccttgacc atgcatccgg catggtacgg tctgttcccg aacggcaggt tgaacgaaag
    19681 gctggccaca agggagacgc tcttgagtgg atggtcgctt atcgcgacgg cgacctcctg
    19741 ggagtccttg acccgaggac cggagtcaca aaggaagtgc ggatcccgag gggaaaggag
    19801 acatctgccg gaaccacggt acgcatcctc gaagacgggg accttctggt cctgctggag
    19861 tgagcctgat gagagtcagg gaaatacccg tcaatgaact ctgcagcctg aaaggcgccg
    19921 actgggtcga ccggaacagg cggacatatc tcgacggcaa cattgccttt gtccctgtca
    19981 gggaaggctg cccgtgtacc tccactctgc cccagaggag accataccag ggcagggggt
    20041 atttcatgct cggggacgtg gcggtcttcc acgggaggcg gcccggagag gacgaggtcc
    20101 ggaacctgat ggagtggaag aggcctgcag gagtcctgtg ggtccagtcc tgcaagggaa
    20161 cgatgagaac cccacaaacg gaggtccttg cagggactgt gcaggaggtc cgccacaggg
    20221 agtgcgggat tacgtactgg ctggaccccg catccatcat gttctccatg gggaaccggg
    20281 acgagaagga gcggatacgc tcccttgtcc atcccggcga gcggattgcc gacatgtatg
    20341 cagggatcgg gtattttacc ctcccttcgg ccctggcagg tgccagggtc catgcaatgg
    20401 agatacggcc cctcgcgttc tcttaccttg agagaaacat catcgggaac gatgtcgccc
    20461 acctcgtaac cgcagagctt ggcgactgca gggactgcct tttggggacc tatgacagga
    20521 tcatcatggg gcatttcggg tccatcgacc gcctcacgga tgccctggcc cattcagggc
    20581 cgggaactgt cctgcacgtc cattcggcag gtgcgctgaa ggacaagatc caggcgatcc
    20641 ttgaagatgc aggtgtcact gccgcaatag cgatccataa ggtaaagaaa ctggcgccgc
    20701 atacctggca ttacgtgcag gacgtgaccc tggaatgagc ggaatcaaca cgctggaagg
    20761 aaaggagatc gtgctcgcgg tgacagggag cattgctgca gtggacaccg tgcgcctcgc
    20821 ccatgccctc cggaggaggg gtgcgcgggt gcaggcagtg atgagcagcg cagcctgcgg
    20881 catcctccac cccgctgccc tgacctatgc gaccgggaga cccgcgatca ccgggattac
    20941 cgggatggtc gagcacgtcg cctggtgcgg tgagggtggc atggcagacc tgctcctggt
    21001 tgccccggcg accgcaaaca ccctctgcaa gatcgccgca ggaatcgacg ataccccggt
    21061 taccacgttt gccacgaccg cggtgggaag ggggatgcca atcgtcgtgg ccccggccat
    21121 gcacgagagc atgttcaggc acccgggggt tgcaggctgc ctgcgcaccc tggaagggtg
    21181 ggggataacg gtggtgggcc cccgtatcga ggaagggaag gcaaagattg ccgatattgg
    21241 ggatattgta ctccattgcg agcgggaatt gatgggaaag cccctcctcg ggcaccacgt
    21301 cctcgtcacc agcgggccct gcagggagag ggtggatgat gtgcgggtcc tcaccacgcg
    21361 ctccaccggg ctgatggggc gggcgcttgc actgcaggcg ttccggcttg gggcacgggt
    21421 gacggtcgtc cacgctgacc ggttccccgc ggtggagaac gtgcgcacca cggacgcggc
    21481 cgggatgctc aaagaagtca cccggatatg cagggaggac cccgtcgatc tcttcatctc
    21541 tgctgcagct gtctcggatt tctgccccga gccctgcccg ggaaagatcc cgagcggaaa
    21601 taccgtcacc ctcaccctca atcccctccc gaagatcatc gatgacgtaa tggaccggtt
    21661 ccatgtgccg acgatcgcct tcaaggtcgg ggatgacgct gtgacaaggg gcaggaccat
    21721 gctcggggac agcatcgggc tcgtggtggc aaacgacccc aacgtcatgg gtgaggttga
    21781 cacccgcgtg accatgctcg ggcagggggg attcgtgcgc gaggcatccg gaagcaaaga
    21841 ggccgttgcc gcccagatac tcgattgcgc gatggagata ttccggtttc cgaaaaaagg
    21901 aagataatcc cctctttttc tgcaggataa gagtatacct tttcaagact ttgcttcagg
    21961 ggctttcgcc cccgcacgct tgcgcaaccc taaatgcgat aaggctggtg ctggggatag
    22021 atcaggatta gtcgcacctg ctcttgctct ccccacagac tatcgcattg acggggggag
    22081 ggggttttct tccctatccc gtcaaagatc tcgtttgatt tgtatcacaa tgcgaacaca
    22141 accgcactca tcacacgatg acgctccagg ggctccgccc cgccgctgcg gcgaccctcc
    22201 caatgcgata ggaccagtgc tggaaataga ccagaatcag aggttttcca gtttgatctt
    22261 cttgcagact atcgtgaatc gggggagggg tcggggagga ggattctccc cctcccccct
    22321 acgcgaaaat cgcactccac acacgatgac gttcttgggg ctaacgcccc cgccattatg
    22381 gcatccccca agcgcgatag agccagtgct ggggacagat caggattagt ctcacctgat
    22441 cttgctttcc ctgcaggcta tcgcggagca gttggggcat tatccccaac tgcggggggt
    22501 tgtacagatg ctggctttgg tgcaatctgt tcatggattg aacacaaccg cactccatac
    22561 acgatgacgt tcttggggct gacgccccgc cgctgtggca tccccatagc gcgatagggc
    22621 cagtgctggg gagtaggtag gattcagaag aatccggtgc tgccctgccc acaggctatc
    22681 gcgtatcagg gggaggtccg gagggggatt ctccccc
//
LOCUS       DAOQYR010000026        22446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_2660_length_22446_cov_8.631459, whole genome shotgun sequence.
ACCESSION   DAOQYR010000026 DAOQYR010000000
VERSION     DAOQYR010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 22446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22446
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2660_length_22446_cov_8.631459"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1841
                     /locus_tag="PKM87_04820"
     CDS             <1..1841
                     /locus_tag="PKM87_04820"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNQ29425.1"
                     /translation="IVIMFLMAAAYAQAYSWTVQQDHEQALFTEMYLEESILLADRGL
                     SLYDSTLDSRMEEAFLGYLSAYHASEGDISRIDLPAIRDALDSGFRGQLDLYIINESG
                     VIVASTVPGVMYLDFKNYPEFFRSITRIREGDEFVADRVVRSVVNTTEGTVSGQLRKF
                     AFYPTPDHRYLLEMGLVMEGFENQRSELSYVEVTRRIQEMNPSIELIRIFDVNGNLLL
                     ERGVNPHTGANQTFIGEIFSTGKDAEILEQDRNRRLHYIYVDLRESSTASDMSLVAEI
                     TYTQEPLRKALSQILLYYTLIGFIAIVMGIVMAYVIARFLTTPITEILEDVSRIAQGD
                     LGHTIRSMPNPEFRRLEESINLMIGKIQQFSREIEREKAEIRIAAEIQRTFLPREIPE
                     SERFEVAAMNIPAQEVGGDFYDFIPLNERSLGLVIADVAGKGIPAALFMALSRSILRA
                     IATADQRVEETIEESNSLIAADAAAGMFVTLFYCTLDKETGEVSYVNAGHNPPLHYSQ
                     KEKRLRTLLPTGMAMGVMPDLSYSVGHLTLETGDILVLYTDGVTEAFNQNDEEFGEGR
                     LMDAAVSSRGMSAGEILDTIYRKVTEFTGGAMQSDDITLVVVRCRR"
     gene            complement(1956..2153)
                     /locus_tag="PKM87_04825"
     CDS             complement(1956..2153)
                     /locus_tag="PKM87_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29426.1"
                     /translation="MTKKEHPGYEKVRCCHCEGAGCVYCDKTGYVLVKSPACLCRHCE
                     GGCCIYCGFTGWAGLKGKYDE"
     gene            complement(2291..3829)
                     /gene="pap"
                     /locus_tag="PKM87_04830"
     CDS             complement(2291..3829)
                     /gene="pap"
                     /locus_tag="PKM87_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HNQ29427.1"
                     /translation="MEQGDRAAGITLPAHVSSREINDDQYHAIHESQRTQAGALQRQA
                     HDLGIPVIILFEGWYGSGISQMVRDLNRTLDPRGFSYHPIAAPGPLAREHHFLWRFWI
                     RTPARGRIAIFDRGWYARTILDRMDPRKESRVSGSYVDEILGFERTLADSGVALVKMF
                     LHISKKEQKQRLRRLKKDPLTMGHLEGDLDRYIRYRKYYSLVEDLLSRTSTGQSPWHV
                     IDAEEKKYTAVTVFDTILRRLEGAIGEAAKSIVHEAKPERATGENQPSIEHSAIGKAD
                     LTLSIDRTEYDSRMRSLGKALAESQIRLYQEKVPLILIFEGWDAAGKGGNIMRVTRSL
                     NPRGYAVMPVGPPDAGERGRHYLWRFFPALPARGHITIFDRSWYGRVLVERIEGLCRR
                     DEWTRAYREIREFEGSLAEDGAIIRKFWLHIDRDEQLSRFHQREADPEKQWKITEEDW
                     RNRARWDEYEVAVDQMIHLTSTPEAPWVVVEANDKYYSRVKTLQAIVDAVDSCLADKS
                     CSLL"
     gene            complement(3837..5495)
                     /gene="ilvD"
                     /locus_tag="PKM87_04835"
     CDS             complement(3837..5495)
                     /gene="ilvD"
                     /locus_tag="PKM87_04835"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNQ29428.1"
                     /translation="MQSLRSDEVKRGYQRAPNRALLRSLGVTDREIDLPYIGIANAWN
                     DIVPGHTHLRMLAGKIREGIAAAGGVPFEFGVIGICDGVAMGHEGMRYSLPSRENIAD
                     AVELMVEAHRFDGMVLLGTCDKIVPGMLMAAARCNIPAIAVTGGAMLPGYQEGRDLSL
                     IDVFEGVGKVAAGSMSEEELVTLECAAMPGCGSCQGLYTANTMACMTEAMGMSLPGCA
                     AMPAVEAAKLRIARESGERVLDLVNEGICPRDIITRRSLENAIRVDMALGGSSNTVLH
                     LMAIAAEAEVDLSLDSFTRIGEQIPHLCNMMPAGPHSMQALHRSGGIPAVLKAISDHL
                     EDSPTVSGRRIKEIAHDVKVPSRSIIRSTRDPVSPAGGLRILFGSLAPQGSVIKSIAV
                     NREMWTHTGPARVFDGEQEAMKAILAREIHEGDVIVIRYEGPKGAPGMPEMLSPTSAI
                     VGLGYSRVALVTDGRFSGGTRGPCIGHVAPEAAAGGPIGLVRDGDRIAIDLHGRTLDL
                     LVDASELGKRKTGWSPKERSLKGVLARYAAMVGQADRGAVLERR"
     gene            complement(5621..6958)
                     /locus_tag="PKM87_04840"
     CDS             complement(5621..6958)
                     /locus_tag="PKM87_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HNQ29429.1"
                     /translation="MIRIPSIPRIVIAGTHSGCGKTTVARGIMEALTRRGYTVQPFKV
                     GPDFIDPTHHSAICRRVSRNLDAFMMGENGVVETFRRASAGAEIAVIEGVMGMYDGVD
                     GGDFASTAHVMRILSAPALLVADARGMSRSVHALVKGFLEFEPGMRIGGVVFNRIGSP
                     RHRELIDCGRTAPAIGYLPRTPGLEIESRHLGLAMAHETAPSAGLGELMEEHCDIDQV
                     IRIAGEAPFLPRSDAEEQKGSARARIGIARDEAFCFYYQDNLDLLERSGARLVPFSPL
                     RDDFPEVDAVYIGGGYPELYAAALESSSCRDRIAKATSDGMPVYAECGGLLYLCESLE
                     TDRTHRMAGVLPADAWMTEKVQALGYSDGDWSGGPTLAPLSGRILGHEFHYSQVECRQ
                     DARFAISLTRGRGIASGKDGLYAGESLAAYTHAYFPASFASSLVEAASAYRRA"
     gene            7083..7523
                     /locus_tag="PKM87_04845"
     CDS             7083..7523
                     /locus_tag="PKM87_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29430.1"
                     /translation="MTPMQKAILAIAVILAAGGIALLAACVHAPGIQHAVPPDQDVFF
                     RSVAGGFEDRIIIHLIPVGGNVETCLASWSVAEDGNILIDRKDQRISRVTTEDHIILE
                     VPRRAQSEYTVNMIIRSPDGRELDNSSLRIGPLAPATQETSGGE"
     gene            complement(7529..9949)
                     /locus_tag="PKM87_04850"
     CDS             complement(7529..9949)
                     /locus_tag="PKM87_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase"
                     /protein_id="HNQ29431.1"
                     /translation="MSSPAGEMGISITQVEYSNTPNGPLIHIFGRDPDGGARHLQVTG
                     FQPYFYAPVDQVRQRPPASHAVPDWESVHTSIKGNRVVRLYTPRPTDVREARDQYQHF
                     EADIPFATRFMIDCGLTGGVIAPGEYADYLEIRPADVYAPARVCMLDIECEDQRGFPE
                     PSRDAIICITCWDSYLDRYATFLYPSPGQEEGALQLGLATPRTTGCFDPSRHKVEVYP
                     SETEMLKGFANYVKQTDPDILSGWNFLEFDLPYIVRRMEALSLSPTMLSRLEGQVERL
                     ALRGRATFDLLPAYRKMHATQKESYRLDAVAEAEVGETKIRYTGTISDLWKRAPCLLV
                     EYNHKDVELCVAINEKNRIIEFYREIARYVGCPLEKTLNSSNVIDIFVLRKAHGRYVL
                     PSKGYAPAEEFEGATVFDPSRGVKENVVVLDLKSLYPMAMMTINASPETKDPQGELRA
                     PNGVRFRRAPDGLTRSIISDLLIERDEKKATRNRYPFGSPEYILYDMQQNVLKVIMNT
                     YYGVSGYTRFRLYDREIGAAITSVGRAIIEHTRKVIESLGSHVIYGDTDSCMVALPGL
                     DREATIRKAREIEATLNASYADFARTELNAETHYFSIKFEKVYKRFFQAGKKKRYAGH
                     LVWKEGATVDEIDIVGFEIRRSDYPQVTKVVQRRVMELILRGEGYDEVKKFLGEVIRD
                     FRAGKYTLDEIGIPGGIGKDLSDYQTDDAHVRGAKYANQHLGMEFAKGSKPKRVYIKN
                     VTSKYQKTDVLCFEYGDQVPPEFQVDWEMMMEKTVKQPISRIIEALGWEWADIDPSRT
                     TLAQWGIG"
     gene            complement(10007..10873)
                     /locus_tag="PKM87_04855"
     CDS             complement(10007..10873)
                     /locus_tag="PKM87_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HNQ29432.1"
                     /translation="MTTLSVLGVGRIGGEIAYLSAILGLADELVMYDAVPAFLHSQVL
                     DLKHTGLDLPVSTDWKDVKQSDICVFSAGLPRNPDVKTRADLLQANLPVAFECSQALR
                     HFDGVLITVTNPMDANNYYLCRKNHLEPSRCIGFGGQLDSARFGLELKSRGIRGEPWV
                     IGDHGEFQVPLFSHLKDPVGNTTRGEILSNLRGASMEIIKGKGGTVFGPAYHIAGLIQ
                     AVAEDRRATLACSCILDGEYGLHGCSIGVPAVIGREGVSEILEWKMDAWEESHFKSAG
                     EFVTGLCRELGV"
     gene            10936..11823
                     /locus_tag="PKM87_04860"
     CDS             10936..11823
                     /locus_tag="PKM87_04860"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNQ29433.1"
                     /translation="MLTIAPGPINVGGVTLGNHLLLAAGILGTTGSSLLRVLSSGAGG
                     VVTKSIGPVPLEGHPGPCLVAVEGGLLNAMGLPNPSMEFLDELAHLAGKPVIASIFGA
                     SPAELAEVAGWFSGKVCAIELNLSCPHASGYGASLGSDPVLVEECTRAVAQKGLPTWV
                     KLTPNVTDIAEIGRAAERGGARAIVAVNTLKAMRISVGLRRPVLGNRVGGLSGKAIFP
                     VAVRCVYDLYEACSIPIIGCGGISTAADVLEMMMAGASAVEIGTAVIDDITIFQKIAA
                     DLYDPRGDPVESVVGCAHG"
     gene            11816..12580
                     /locus_tag="PKM87_04865"
     CDS             11816..12580
                     /locus_tag="PKM87_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNQ29434.1"
                     /translation="MAEQLPVPVRITGVTSDTPNVRTFTFDQEFGFVPAQFVMVWVPG
                     VDEIPMALSSSSSITVQRVGDATSALFSLSEGDMIGIRGPFGNGFPPARRILAIAGGV
                     GAAPLLPVVRAGGVRKLLLGARSAEELLFHDIFREHTELAVSTDDGTYGHRGFVVDLL
                     AGENLGSHDCIFCCGPERMMAAVLAYLARSGCPEKGFFSLHRYMKCGVGVCGSCAIDP
                     QGLRVCRDGPVFRGDILLKSEFGHYSRDASGRRRPV"
     gene            12786..13664
                     /locus_tag="PKM87_04870"
     CDS             12786..13664
                     /locus_tag="PKM87_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNQ29435.1"
                     /translation="MVFHPPVAIVAKAGDAGKYKVGLPSWNMLLRGFMAGAYIAMGGA
                     LATMCSTGIVATDAAMRYGTASAGFGQLITGAVFPVGLIIIVLTGAELFTGDAMLAPM
                     AAFIHKISWGSVLNLWVWVYIGNLIGSIWYAYLMAYGPFCTVDAAGLITANAFGSRAI
                     AIAAAKVSYVSMAAHWSAFLKAIGCNWLVNLAVLLGICADDLIGKFFGIWFPIMAFVS
                     SGFEHCVANMYFIPAGVMTQGFITDPTKINAGLNWMTFITNNLIVVTIGNIVGGLFFV
                     GVIYWVAFRKEIAALK"
     gene            13675..14205
                     /locus_tag="PKM87_04875"
     CDS             13675..14205
                     /locus_tag="PKM87_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HNQ29436.1"
                     /translation="MKIGKIRLKISPIILVVGGFFAILLAIAYILGRDSTLLVWGIPT
                     LILLMIMPLGLNYLSQKEYADVRPQYEREARSVRIKLINLNMVGEPVRIEGVVEKVRF
                     QFLNRPQYIVADRSGEISVKMFTHPGVTIKVGDVVEVLGMVMKRYLAAGDAIVNGVSI
                     KKIDKVVEVKPQAKGK"
     gene            14331..16121
                     /locus_tag="PKM87_04880"
     CDS             14331..16121
                     /locus_tag="PKM87_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNQ29437.1"
                     /translation="MNYGGTTFQRLLMIAIVVGVVSGFGSLLFFEGLTIATQFFMGTC
                     LGYSYPVEGQSAAEIAGWAPPPNIWVLLPIICFGALASGILVTKLAPETEGHGTDAAI
                     RAFHGEGTIRKRVPFLKAITAILTISTGGSAGREGPTAQIAAGIGSMIADMLKLPLHE
                     RRMALNIGIGAGIGTIFLAPLGGAILAAEILYRRDFESEVIVPAFLASIIGYSIFGLF
                     EGFSPVFAEAPIVWNVFQIPFFLVLGLICAGYGWLYIHTFYRSHEVFADLVRKYHFPP
                     FIKPVIGAFLTGLIVIILFLFIPNGAEIGLGSLGSGYGFVQLALYSMLPIGVLVALSF
                     LKILTTSLTIGSGGSGGVFAPGLVIGGMVGGMCGVIFNILFPAYFPVETIPAFVIIGM
                     IALFGCVSYAPIAVMIMVVEMTGNFSLLVPAMGAVAIACLFTRGSGIYRAQVETKADS
                     GAHRGEYEVSILQKIPAGTSMTPAEKVLVLHPEDKSREVLHLIRLHGHNGFPVIDNGK
                     LVGIMTIQDVRQVRKKQDLDAPVKDGMSRDLFTLTPDTTLDNALKIMIERDINHIPIV
                     DTIESQKLVGFLTRTDIMNAYARYSEENSL"
     gene            16242..16652
                     /locus_tag="PKM87_04885"
     CDS             16242..16652
                     /locus_tag="PKM87_04885"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNQ29438.1"
                     /translation="MNRIIHFEIQADEPEQVAKFYRSVFGWEINEWVLPGFEMKDEDR
                     YWIVITGAEGEPGINGGLVFRHGPRAPGGLSANAFFCTIGVADLDKSVKKVTDAGGKI
                     VVPRMAIQGVGWWVSCLDPEGNQFGMMQEDPDAG"
     gene            16810..17901
                     /locus_tag="PKM87_04890"
     CDS             16810..17901
                     /locus_tag="PKM87_04890"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HNQ29439.1"
                     /translation="MNSSEILSLRLSNSGLSHSPFQSPTDAVTQLAAVQAQDFTAAKW
                     SLGLRVKKSTDADIERAFNEGRIQRIHVMRPTWHFVMPEDIRWMLELTARRVKSLLAA
                     SNRRIGLDEALLAKSNAAIVKALEGHRYLTRQEMKTVLNDIGIRTDVRQLAHAIMQAE
                     LDGLICSGPKRGKQFTYALLDERVRKSARISREEALKKITLRYFTSHGPAQQKDFSWW
                     SGLAEKDAENAIELIGSHLDRKIVNGKEYWFAPREPGEIPDPPTALLLSVYDEYTIAY
                     KDRSDLSEAREIERMIARGNSFPAVLILDGKVAGTWNKRMKSQALEIRLDPFRILDDE
                     EQEAVEAEASRYGRFFGVPVIFVMEPSGR"
     gene            complement(17964..18737)
                     /locus_tag="PKM87_04895"
     CDS             complement(17964..18737)
                     /locus_tag="PKM87_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ29440.1"
                     /translation="MDLEQFFREHGIDVFSEVGLDALSIDDQSSIREFFPDALSVIVF
                     GKEVPMDVYRMPAGEKTRGMFQIAESVEQAALNLTRLFFAEQSAARPVPLLFPIRIVE
                     GRLMGLVRLKQIAEAGGLGSIGKSTLLLTPQFGPRVILGGVVTSRPAQKTGGFKRPES
                     SLCTGCERCIRACPGHAIGGDGVDAFRCQSLSAWIPGCLVPAAKWMLRRALLLRFVGP
                     LVPWIARYAAMPCSMCVTECPVCVTDTEKGKLSDPGHYV"
     gene            18854..19633
                     /locus_tag="PKM87_04900"
     CDS             18854..19633
                     /locus_tag="PKM87_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HNQ29441.1"
                     /translation="MNDTKIIDLTPDTIADYGVCGYKDARKHVELQRKIAWFKKYYTK
                     GLRIKALVSETGGYQGMLEYMPGEYAHRPVDADGYMFIQCIFVGFRNEFKGKGYASSM
                     IDACIKEARDAHMKGVAVVTRKGSFMAKRDIFLKYGFSPADTAEPDFELLVLKFDPDT
                     ANPRFKPTLKKNRDLYADGLTIFRSPQCPYSEKNVKAIMETARKKYGLSPRLVELEDA
                     ESVQNSPCAFGTFCIVYNGEVISHHPISNGRFVNIMDKIRK"
     gene            19653..20063
                     /locus_tag="PKM87_04905"
     CDS             19653..20063
                     /locus_tag="PKM87_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HNQ29442.1"
                     /translation="MNATIRGRCGFLCSNCPAYKENIHSDSDRERVSVVFQKLYGHII
                     EPEGVYCDGCLEPDENNIRRLGTERCPMRDCVLDKNIPHCGKCSVFPCELMERHLATV
                     ETVVTKARTMLTPEEYRDFVEPYLSREFLSGKTG"
     gene            20175..20459
                     /locus_tag="PKM87_04910"
     CDS             20175..20459
                     /locus_tag="PKM87_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNQ29443.1"
                     /translation="MDVLTLLRQHEPELKSRFGVAKIGIFGSYARGEERLDSDVDVLV
                     IFQKGKKTFDNFMGTKFYLEDLFRRRVDLVTEAALKPLIRDPILKDVLYA"
     gene            20452..20802
                     /locus_tag="PKM87_04915"
     CDS             20452..20802
                     /locus_tag="PKM87_04915"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNQ29444.1"
                     /translation="MPRSQLLYLNDIFEAIQNIRLYVGALTLGEFQNDQMRVDAVVRN
                     FEVIGEAVKNLSDELKTQFPDTDWKAVAGFRDTLIHGYFGIDLEILWDIISNKIPVLE
                     KEVADMIAYENAKN"
     gene            21198..21455
                     /locus_tag="PKM87_04920"
     CDS             21198..21455
                     /locus_tag="PKM87_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29445.1"
                     /translation="MGIFDIFKNKKQVNDEKVQIIPQNKSNIKINSSHSLVPYFGNLI
                     IPENIRHLLWFGDGIFQNYNPDNDHRGFRKTRFELQELSLS"
     gene            21470..22415
                     /locus_tag="PKM87_04925"
                     /pseudo
     CDS             21470..22415
                     /locus_tag="PKM87_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315771.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         5039 a         6196 c         6084 g         5127 t
ORIGIN      
        1 tcattgtgat catgttcctg atggccgccg catatgcgca ggcctattca tggacggtgc
       61 aacaggatca cgagcaggcg ctgttcaccg agatgtacct ggaagagtcg attcttctcg
      121 ctgacagggg cttaagcctc tacgacagta cgcttgattc ccggatggag gaggcgttcc
      181 tggggtacct gtcggcatat cacgcatcag aaggtgacat cagccgcatt gacctgcccg
      241 ccatacggga cgccctggat tccggctttc gggggcagct cgacctctac attataaacg
      301 agagcggggt tatagtcgcg tccacggtcc ccggagttat gtatctcgat tttaaaaatt
      361 accccgaatt tttccgctcc atcacccgta tccgtgaagg cgacgaattc gtagccgaca
      421 gggtggtccg ctctgtcgtg aacacgacgg aaggcaccgt ctccgggcag ctccggaaat
      481 ttgcttttta tccaacgccc gatcaccgct accttttgga gatgggcctt gtcatggagg
      541 gattcgagaa ccagcggtcg gaactctcct atgtcgaggt gacgagacgg atacaggaga
      601 tgaacccatc catcgaactg atacggatct ttgacgtaaa tggaaacctc ctcctggaac
      661 ggggggtcaa cccccacacg ggtgcaaacc agacattcat tggagagatc ttttcgaccg
      721 ggaaggacgc ggagatcctg gagcaggatc ggaaccggcg cctccattat atctatgttg
      781 accttcgcga gtcatcaacc gcgtctgata tgagcctagt cgcagagatc acctataccc
      841 aggaaccact gcgcaaggca ctctcacaga tcctcctcta ttacaccctc attgggttca
      901 ttgccatcgt aatgggtatc gtcatggcct atgtcatcgc ccggtttctc accacaccca
      961 tcaccgagat actggaggat gtcagcagga ttgcacaggg cgacctcggc cacaccatcc
     1021 ggagcatgcc gaaccccgaa ttccggaggc tggaggagag catcaacctg atgatcggca
     1081 agatacagca gttctcccgg gagatcgaac gagaaaaggc agagatcagg atcgccgccg
     1141 agatacagag gaccttcctg cccagggaga tccccgaatc ggagaggttc gaggttgccg
     1201 caatgaatat cccggcgcag gaggttggtg gcgacttcta cgacttcatc cccctaaacg
     1261 agcgcagcct tgggctggtc atcgcagacg ttgccgggaa ggggattcca gccgcactct
     1321 tcatggccct ctcccgctcg atactccgtg caatcgcgac cgccgaccag cgcgtggaag
     1381 agacgattga ggaatcgaac agcctgattg ctgccgatgc tgccgcaggg atgttcgtga
     1441 cgctctttta ctgcaccctt gacaaggaga caggcgaagt cagttacgtg aatgccggtc
     1501 acaacccccc gctgcattac tcacaaaagg agaaacggct gcgcaccctt ctccccacag
     1561 ggatggcaat gggtgtgatg ccggatctga gctactcggt gggccacctc acccttgaaa
     1621 caggggacat acttgtcctc tacactgacg gggtaacaga ggcattcaac cagaatgatg
     1681 aggagttcgg ggaagggagg ctcatggatg cagcagtctc gtccaggggc atgagtgccg
     1741 gggagatact cgataccatt tacaggaaag tgacagagtt taccgggggt gcgatgcagt
     1801 ccgacgatat cacgctggtg gtcgtcaggt gcaggagata atgtctccga ttgggccagg
     1861 caacccagga ctgccgccct gtttcctgcg gttgaatgcc cagaaaaaaa atattatacg
     1921 gctatcagcc agagtcgacc tgtgtgagcg cgtggctatt cgtcatattt ccctttcagt
     1981 cctgcccaac ccgtgaaacc gcagtagatg cagcagcccc cctcgcaatg cctgcacagg
     2041 catgcagggg atttcaccag cacgtacccg gtcttgtcac agtagacgca gcctgcccct
     2101 tcgcagtggc agcagcggac cttttcgtat cccggatgtt ccttttttgt cattcttctc
     2161 agggtagtgt cggcaggcac ggggttaagc atttgcaaaa gggtcaggaa tggcgtgcag
     2221 tcttttccat tcatgaacac ccgggcaacc atgccgggaa aaaggaggag ggtgcgaacc
     2281 gccggcaccc tcacaagagg gaacaggatt tatctgcaag gcagctgtcg actgcgtcga
     2341 caatggcctg gagtgtcttc acccgggaat agtacttatc attcgcttcc actacgaccc
     2401 agggggcttc tggagtgctt gtgaggtgaa tcatctggtc caccgcaact tcatactcgt
     2461 cccatcgtgc gcggttcctc cagtcctctt cggttatctt ccactgtttt tcagggtctg
     2521 cttcacgctg gtggaaccga ctgagctgct cgtcccggtc gatatgcagc cagaacttcc
     2581 tgataattgc cccgtcttct gcaagagagc cttcaaactc cctgatctca cggtatgccc
     2641 tcgtccattc gtcccttctg cagagacctt cgatacgttc tacgagcacc ctgccgtacc
     2701 agctgcggtc gaagatggtg atgtggcccc tggcagggag cgcagggaag aaccgccaga
     2761 gatagtggcg gccacgttcg ccagcgtccg gaggcccgac aggcatcact gcatatcccc
     2821 tcgggttgag cgagcgggtc accctcatga tattcccccc ctttcctgct gcatcccatc
     2881 cttcgaagat gaggatgaga gggactttct cctggtaaag ccttatctgg ctctccgcga
     2941 gggcctttcc aagcgatctc atgcgggaat cgtattctgt cctgtctatc gagagggtga
     3001 gatcggcctt cccaattgca gaatgttcaa tcgagggttg gttttcgccg gtggctctct
     3061 ctggttttgc ctcatgaaca atgctctttg cagcctcgcc gattgcccct tccagcctcc
     3121 ggagtatcgt gtcaaagacg gtcactgcgg tatatttctt ctcttccgca tcgatcacgt
     3181 gccagggcga ctggccggtg ctggtacggg agaggaggtc ttctacgagg gagtagtact
     3241 tgcgatacct gatataccgg tccaggtcgc cttcgaggtg acccatcgtc agcgggtctt
     3301 ttttgagcct ccgcagcctc tgcttctgct cttttttact gatatgcaga aacatcttca
     3361 cgagcgcgac tccgctgtct gcaagggtgc gctcgaagcc gaggatctcg tcaacatatg
     3421 aaccggatac acgactctct ttcctggggt ccatccggtc gaggatggtc cgtgcatacc
     3481 agcccctgtc aaagatggca atccgccctc ttgcaggagt tcttatccag aaacgccaga
     3541 gaaaatggtg ctctcttgcg agtgggccag gtgctgcgat gggatgatac gagaatcccc
     3601 gggggtcaag ggtacggttg aggtcacgca ccatctgtga tatccccgat ccgtaccacc
     3661 cctcaaagag gatgatgaca ggaataccca ggtcatgtgc ctgccgctgg agggcccctg
     3721 cctgggttcg ctgggattcg tggatggcat ggtactggtc atcgtttatc tcgcgggacg
     3781 aaacgtgcgc agggagagtg ataccggcag ccctgtcccc ctgctccatc agctcctcac
     3841 cgtcgctcca gcactgctcc cctgtctgcc tgtcccacca tcgcagcgta tcgtgcgagc
     3901 acgcctttca gggacctctc cttcgggctc caccctgtct tccgctttcc aagttcggat
     3961 gcatcaacga ggagatccag cgtccgtcca tggaggtcga tcgcgatccg gtctccgtcc
     4021 ctgacgagcc cgatcggccc gcctgcagcc gcctccggtg ccacgtggcc gatgcacggc
     4081 ccacgcgtcc cgccagagaa cctgccatct gtcacgagtg caacgcgcga ataaccgagg
     4141 ccaacgattg ccgacgtggg ggagagcatc tcgggcatcc ctggtgctcc cttcggcccc
     4201 tcgtacctga tcacgattac gtccccctcg tggatctccc gggcaagaat cgccttcatg
     4261 gcctcctgtt caccatcaaa tacccttgcc ggcccggtat gcgtccacat ttccctgttc
     4321 acggcgattg acttgataac tgatccctgc ggtgcaaggc tcccgaagag gatcctcagt
     4381 cccccggcag gactgacagg atctcgtgtg ctcctgatga tactccggga aggaaccttc
     4441 acgtcatgcg cgatctcttt gatccgtctc cccgaaactg tcggggagtc ctcaaggtgg
     4501 tcagatatcg ccttcaggac cgctggaatg ccccccgacc ggtggagtgc ctgcatagaa
     4561 tgcggcccgg caggcatcat gttgcagaga tgagggatct gctcccctat ccttgtgaaa
     4621 gagtcaagcg aaagatccac ctccgcttct gccgcgatgg ccatcaggtg gagaaccgtg
     4681 ttcgaagacc ctccaagcgc catgtccacg cggatggcgt tctcgaggct ccggcgggtg
     4741 atgatatccc gggggcaaat cccctcgttc acgagatcaa ggacccgttc gccggactcc
     4801 cttgcaattc tcagtttcgc cgcttccacc gcaggcatgg cggcacagcc gggaagagac
     4861 atccccatcg cctcggtcat gcaggccatg gtgtttgcag tataaagtcc ctgacagctg
     4921 ccgcagcctg gcatcgccgc gcattccagg gtcaccagct cctcctcgct catgctgccc
     4981 gcagcaacct ttcccacccc ttcgaagaca tcgataagcg agagatcgcg gccttcctgg
     5041 taacccggga gcatggctcc gccagtgacc gcaattgcag ggatattgca gcgggccgcg
     5101 gccatcagca tgccaggcac gatcttgtcg caggtcccga gcaggaccat cccgtcgaac
     5161 cggtgtgcct ccaccatcag ttccacggca tctgcgatat tctcgcgcga ggggagggaa
     5221 taccgcatcc cctcgtgacc catcgctacc ccgtcacata tcccgatcac gccgaactcg
     5281 aacggtaccc cgccggcggc tgcgataccc tcgcggatct ttccggccag catccggagg
     5341 tgggtatgac ccgggacgat atcgttccag gcatttgcaa tcccgatgta agggaggtca
     5401 atctcgcggt cagtgacccc aagggaccgg agaagcgccc ggttcggcgc cctctggtac
     5461 cctctcttca cctcgtcact ccgtaatgac tgcattgtct tttaatctcc tctgcatgag
     5521 ttctcttctt cgggtataat caaatgcgca cctcgcatcc gcgctgcttg cagggtttcg
     5581 attcaggaaa gcgggcaaga tgccaaagga gatggagcaa tcaggccctg cgataggcgg
     5641 aagctgcctc tacgagcgaa ctggcgaatg aagccgggaa ataggcatgg gtgtaggccg
     5701 caaggctttc gccggcatac agcccgtctt ttccgctcgc aattcccctg ccgcgtgtca
     5761 gactgatggc aaacctcgcg tcctgccggc attcaacctg tgaatagtgg aactcgtgac
     5821 cgaggatccg gccggacaat ggtgcgaggg tgggtccgcc cgaccagtca ccatccgagt
     5881 atccaagggc ctgaaccttt tcggtcatcc aggcatccgc cggcagaacc cctgccatcc
     5941 tgtgcgtcct gtcggtttca agcgattcgc agaggtagag gagcccccca cactcggcat
     6001 agacaggcat tccatccgat gtggccttcg caatccggtc ccggcaggat gaggattcca
     6061 gcgcggctgc atagagctcc ggataccctc ctccgatata taccgcatcc acttcgggga
     6121 aatcgtccct gagcggtgag aatggtacga gtcgggctcc tgatcgttcg aggaggtcaa
     6181 ggttgtcctg gtagtaaaaa cagaatgcct cgtccctggc aataccaatc cttgcccggg
     6241 cacttccctt ttgctcctct gcgtcacttc gaggaagaaa tggagcttcc cccgcaatcc
     6301 ttatcacctg gtcgatatcg caatgttcct ccatcaactc gccgagacct gcggagggtg
     6361 cagtctcgtg tgccatcgcg aggcccaggt ggcggctctc tatctcgagg ccgggcgtcc
     6421 ttgggaggta cccgatggca ggggcggtcc ggccgcagtc gatcaactcc cggtgccttg
     6481 ggctcccgat ccggttgaag accacccccc cgatcctcat tcccggttcg aactcgagga
     6541 accctttcac aagggcatgg acgctccggg acatcccccg ggcatccgcg accaggagtg
     6601 caggtgccga gaggatccgc atcacgtgag cggtgcttgc gaagtcgccc ccgtccacgc
     6661 cgtcatacat tcccatcact ccctcgatga ccgcgatttc cgcgcctgct gatgcacgtc
     6721 tgaaagtctc cacaaccccg ttctcgccca tcatgaatgc atcgaggttg cgtgaaaccc
     6781 ggcggcaaat cgcagaatgg tgcgtcgggt cgatgaagtc aggacccacc ttgaagggct
     6841 gaaccgtgta tcccctgcgg gtcagcgcct ccatgatccc gcgcgcaact gtggtcttcc
     6901 cgcacccgct gtgggtcccg gcaatgacaa ttctcgggat tgacggtatc cgtatcacct
     6961 ctcaccttgt aggtcgccgt attgggtatt gaaaggtgtc ctcctcctgg aaggcccagg
     7021 acggctgcga aaaaatgaca accctggtaa atttcttctt tttttgcgcc gattcctgca
     7081 gtatgacacc gatgcaaaag gcaatactgg caatcgcggt catccttgct gccggaggca
     7141 tcgctctcct tgccgcatgc gttcatgcac ccggtatcca gcatgcagtc ccgcccgacc
     7201 aggatgtctt tttccggagc gttgcagggg gtttcgagga ccgcatcatc atccatctca
     7261 tccccgttgg cgggaacgtg gagacctgcc ttgcaagctg gagcgtggcc gaggatggca
     7321 atatcctcat cgaccggaaa gaccagagga tctcacgcgt aacgacagag gaccatatca
     7381 tcctcgaggt tccgcggaga gcacaatcgg aatatactgt caacatgatc atccggtcac
     7441 cagatggcag ggaactggat aacagttcac tgagaatagg gccccttgca ccggccactc
     7501 aggagacttc gggaggcgaa tagccgcctc acccgatccc ccactgtgca agtgtcgtcc
     7561 gggatgggtc gatgtcggcc cactcccagc caagcgcctc gatgatccgt gagatcggct
     7621 gcttcaccgt cttctccatc atcatctccc agtccacctg gaactccggg ggcacctggt
     7681 ccccgtactc gaagcagagt acgtccgtct tctggtactt cgaggtcacg tttttgatgt
     7741 acacccgctt tggcttgctg cccttggcaa actccatccc aaggtgctgg tttgcatact
     7801 ttgcgccccg tacatgggcg tcgtccgtct ggtaatctga aaggtccttt ccgatcccgc
     7861 ccggtatgcc gatctcgtca agggtatatt tccctgcccg gaagtcccgg atcacctccc
     7921 ccaaaaactt cttgacctcg tcgtatcctt ccccgcggag gatcagctcc atcaccctcc
     7981 gctgcacgac cttcgtgacc tgggggtagt cgctcctccg tatctcaaag ccgacgatat
     8041 cgatctcgtc aaccgtggcg ccctccttcc agacgaggtg ccccgcgtat cgttttttct
     8101 tcccggcctg gaagaaccgc ttgtaaacct tctcgaactt gatagagaag taatgggtct
     8161 cggcgttcag ttccgttcgc gcaaagtccg cataactcgc gttcagagtc gcctcgatct
     8221 ctctcgcctt cctgatggtc gcctcgcgat cgaggccggg aagcgccacc atgcaggagt
     8281 cggtgtcccc gtagatcacg tgcgagccaa gggactcgat cacctttctc gtgtgctcga
     8341 tgatggcacg ccccacggaa gtgattgcag ccccaatctc cctgtcatac agccggaagc
     8401 gcgtgtaccc gctcacgcca tagtatgtgt tcatgatcac cttgaggaca ttctgctgca
     8461 tgtcgtagag gatgtactcc ggtgacccga agggataccg gttgcgcgtt gccttcttct
     8521 cgtcccgctc aatgaggagg tcgctgatga tgctcctggt gaggccgtcc ggggccctcc
     8581 tgaaccgcac tccgttcgga gcgcggagct ccccctgcgg atccttcgtc tcgggagagg
     8641 cattgatggt catcatggcc atcgggtaga gggacttgag gtcgaggacc accacgttct
     8701 ccttcacccc gcggctcggg tcaaagactg tcgccccctc gaattcctcc gcgggggcat
     8761 accccttgga tgggaggacg tagcggccat gagccttccg gagcacgaag atgtcaatga
     8821 cgttcgagga gttgagggtc ttttcgagcg ggcagccgac ataccttgcg atctcccggt
     8881 agaactcgat gatccggttc ttctcgttga tggcgacgca aagctcgaca tccttgtggt
     8941 tgtactccac aaggaggcag ggggccctct tccagaggtc cgagatcgtc cccgtgtatc
     9001 gtatcttggt ctcacccacc tccgcctccg cgaccgcgtc gagccggtac gactccttct
     9061 gggtcgcgtg catcttacgg taagcaggca ggaggtcgaa cgtcgccctt ccgcgcaggg
     9121 cgagcctctc cacctggccc tccaggcggg agagcatggt aggagagagg gagagcgcct
     9181 ccatcctccg gacaatatag gggaggtcga attcaaggaa gttccacccc gagagaatat
     9241 caggatcggt ctgcttcacg taatttgcaa aacctttcag catctcggtc tctgaagggt
     9301 acacctccac cttgtgtcgc gatgggtcga agcagcctgt cgttcttggc gtcgctaatc
     9361 ccaactgcag tgcaccctct tcctgcccgg gagaagggta gaggaaggtc gcatacctgt
     9421 caaggtatga gtcccagcag gtgatgcaga tgatggcatc ccgggagggt tcaggaaacc
     9481 cccgctggtc ctcgcactcg atatccagca tgcatacccg cgccggtgcg tagacatcgg
     9541 caggccggat ctcaaggtaa tccgcgtatt cccccggcgc gatcactccc ccggtaaggc
     9601 cgcagtcgat catgaaccgg gttgcaaacg ggatgtctgc ctcgaagtgc tggtactggt
     9661 ccctcgcctc gcgcacatca gtcggccgcg gggtgtagag gcggaccacg cggttcccct
     9721 tgatagaagt gtgcaccgat tcccagtcgg gaacggcgtg tgatgccggg ggtctctgtc
     9781 gtacctggtc gaccggagca tagaaatatg gctggaaccc cgtcacctgc aggtgccgcg
     9841 cgcccccgtc tgggtccctc ccgaatatat ggatgagtgg gccattcggg gtattcgagt
     9901 actctacctg ggtaatagag atccccatct ctccggcagg ggaactcatt gggttgggtc
     9961 ctttctcctg cgcttccgtg ctccggccac cggaatccat ggtgcgtcag accccgagct
    10021 ccctgcagag gccggtcacg aactcgcccg cgctcttgaa atgcgactcc tcccatgcgt
    10081 ccatcttcca ctcgaggatc tcgctgacac cctccctccc gatgaccgcg gggaccccga
    10141 tagaacatcc atgcagacca tattcgccgt caaggatgca cgagcacgca agagttgccc
    10201 tccgatcttc ggctacggcc tggatcagac ctgcaatgtg gtaggccgga ccaaataccg
    10261 tgcctccttt ccccttgatt atctccatgc tggctccgcg caggttcgaa aggatctccc
    10321 ctcttgttgt gttcccgaca ggatccttca ggtgggaaaa gagcggcacc tggaactctc
    10381 cgtggtcccc aatcacccag ggctcgccgc gaattcccct cgacttcagc tcaagcccga
    10441 accgtgcaga gtcgagctgg cccccgaacc cgatgcagcg ggacggttca agatgattct
    10501 ttctgcacag gtagtagttg ttcgcatcca ttggattggt gacggtgatg agcactccat
    10561 cgaaatgccg gagcgcctgg ctgcattcga acgcgacagg gaggtttgcc tggaggaggt
    10621 cggctcttgt ctttacgtct gggttgcggg gaagtcccgc cgaaaaaaca catatgtcgc
    10681 tctgtttcac gtccttccaa tcggtgctca cggggagatc gagccccgta tgcttgagat
    10741 ccaggacctg ggaatgcagg aacgcaggga ccgcgtcata catgaccagt tcgtccgcaa
    10801 gacccagaat tgccgagagg tacgcaatct ccccccctat cctgcctact cccagcaccg
    10861 atagtgtcgt catactcttc tatcattctc cgtccggaga ataaataatt acaatcacgc
    10921 tgatctcttc tctacatgct gactattgca cccggcccca tcaatgtggg gggtgtaacg
    10981 ctgggaaacc atctcttgct ggccgccggt atcctcggta ccacgggctc gtctcttctg
    11041 cgggtccttt catccggcgc gggaggggtg gtcaccaagt ccatcggccc ggtcccgctc
    11101 gagggccacc ccgggccctg cctcgtcgcg gtggaggggg gcctcctgaa cgcaatgggc
    11161 cttccgaacc cctcaatgga gtttctggac gaactcgcac accttgcagg aaaacccgtg
    11221 attgccagca tattcggggc aagcccggca gaattagccg aggttgcagg gtggttctcg
    11281 ggtaaagtct gtgctatcga gctgaacctc tcctgccccc atgccagcgg atacggggcg
    11341 tcgcttggaa gcgatcctgt cctcgtggaa gaatgcaccc gcgcagttgc gcaaaaagga
    11401 ttacccacct gggtcaagct gacgccgaac gtgaccgaca tcgcggagat cgggagggca
    11461 gcagagcggg gcggggcgag ggcaatcgtg gcggtcaaca ccctgaaggc catgcgtatc
    11521 tcggtcgggc tgcggcggcc ggtacttggc aaccgcgtgg gcggcctctc tggaaaagca
    11581 atattccctg tcgcagtgcg gtgcgtatat gatctctacg aggcctgttc catacctatc
    11641 atcgggtgcg ggggtatttc cactgcagca gatgtcctgg agatgatgat ggcaggcgcc
    11701 tccgcagtgg agatcggtac tgccgtcatt gatgatatca cgatatttca aaaaattgcg
    11761 gctgatctct atgacccccg aggagaccct gtcgagagcg tagtggggtg tgcccatggc
    11821 tgaacagctc cccgtcccgg tccggattac aggggttacc tctgatactc cgaatgtcag
    11881 gacatttaca tttgaccagg agttcggatt tgttcccgcc cagttcgtga tggtctgggt
    11941 cccgggagtg gacgagatcc ccatggcact ctcgtcatca tcgtcgatca cggtccagcg
    12001 cgtgggggat gcaacctctg cgctcttttc cttaagcgaa ggcgacatga tagggataag
    12061 agggcctttt ggcaatggct ttcctcccgc ccgccgcatc cttgcaattg cgggaggtgt
    12121 cggcgcggcc ccactcctac ctgtcgttcg cgcggggggt gtcagaaagc tgcttcttgg
    12181 tgcgcgttcg gcagaggaac tcctcttcca tgacattttc agggaacata ccgaacttgc
    12241 cgtctctact gacgatggga cgtacggcca caggggtttt gtcgtcgatc tgctcgccgg
    12301 agagaatctc ggatcgcatg actgcatatt ctgctgtggc ccagagagaa tgatggcagc
    12361 ggtcctcgcg tacctcgcac gatcaggatg tccggagaag ggcttcttca gcctgcacag
    12421 gtatatgaag tgcggggttg gcgtctgcgg gtcatgcgcc atcgaccccc agggcctgcg
    12481 ggtctgcagg gatggcccgg tgttccgggg ggatattctc ttgaagagtg aattcggaca
    12541 ttattcccgc gatgcaagcg gtaggcggcg ccctgtctga tgcatcgtgg gaagagcggc
    12601 aaacagtaat gaatagtact ccttttctct aatattttaa tttaaaatat tgcaaaacgc
    12661 caatgcagtg caaaaatcat ttagaaaggt attaataaca ccagaaagga ttttaatttt
    12721 gaacctatac gcgaagtgaa ttcgtgggtt aggtataggt aggacattcg agaggtgtgt
    12781 aaaaaatggt gtttcatccc ccagtcgcaa tcgtcgcaaa ggccggcgat gctgggaagt
    12841 acaaagtagg actcccctca tggaacatgc tccttcgtgg attcatggcc ggtgcgtaca
    12901 tcgcaatggg aggcgccctc gcaactatgt gcagcacagg gattgtagcc accgatgcag
    12961 caatgcgtta tgggactgcg agcgctggtt ttggccagct cattacgggt gctgtgttcc
    13021 ctgtggggct tattatcatc gtgctgaccg gtgcagaact cttcactggc gacgcgatgc
    13081 tggccccgat ggcggcattc atccacaaga tcagctgggg ttcagtgctt aacctctggg
    13141 tctgggtcta catcggaaac ctgattggat cgatctggta tgcatatctc atggcatatg
    13201 ggccattttg cactgtagac gccgccggac tgattactgc aaatgcgttc ggatcacgcg
    13261 caatcgccat cgcagctgca aaagtaagct atgtgagtat ggcagcgcat tggtctgcgt
    13321 tcctgaaggc aatcggttgt aactggcttg tcaacctggc cgtgctgctc ggtatctgcg
    13381 ccgacgacct gattggaaaa ttcttcggga tctggttccc gatcatggcc ttcgtctcgt
    13441 ccggattcga acactgtgtc gcgaacatgt atttcattcc tgccggtgtt atgacgcagg
    13501 ggttcatcac cgatcccaca aagatcaatg ccgggctgaa ctggatgaca ttcattacga
    13561 acaaccttat cgtggtcacc atagggaaca tcgtcggtgg tttgttcttc gttggcgtca
    13621 tctattgggt tgcgttccgc aaggaaattg cagcactcaa gtaaacggaa ttgaatgaag
    13681 atcggaaaaa taaggctgaa gatttccccc atcatcctcg tggtgggagg attctttgcc
    13741 attcttttag caattgcata tatcctcggg agggattcca cactcctcgt gtggggtatc
    13801 ccgacgctga tcctcctcat gatcatgccg cttgggctca attatttgag ccagaaagaa
    13861 tacgctgatg tgagaccgca atacgaacgc gaagccagat ccgttcgaat aaaacttatc
    13921 aatctgaaca tggttggtga accggtccgg attgaaggag ttgtggagaa ggtccgtttt
    13981 cagttcttaa accgtcccca atacatcgtt gccgaccgca gcggtgagat ctccgtgaaa
    14041 atgttcaccc atcctggcgt gacgatcaag gttggggatg tcgttgaggt cttgggaatg
    14101 gttatgaagc ggtatcttgc agcgggtgat gcaatagtga acggggtatc gatcaagaag
    14161 atagacaagg tcgtggaagt taagcctcaa gcaaaaggga aataattcgg cttccctttt
    14221 tcagagtcag aattctcctt gctcatatct ttccttggtt gttgatccat attccgaccc
    14281 ccaatttaaa tcgaaatgta attaatttat gggattgtta gtacgataac atgaactacg
    14341 gtggcactac ctttcagcga ttactcatga tcgccatcgt ggtaggagtg gtatccggtt
    14401 ttgggtcgct tcttttcttc gagggtctca ctatcgcaac ccagtttttt atgggtacgt
    14461 gcctcggata ttcataccct gttgaaggac aatccgcagc cgaaattgca ggttgggctc
    14521 cgccacccaa tatctgggtc cttctcccaa tcatctgctt tggagcgctc gcttccggaa
    14581 tacttgtcac aaaacttgct cctgagaccg aggggcatgg taccgatgcc gccatcaggg
    14641 ccttccatgg cgagggaacc atacggaagc gcgtcccttt tcttaaagcg atcaccgcca
    14701 tcctgaccat atcgaccggt ggtagtgcag gaagagaggg gcctacagcc cagattgcgg
    14761 ccggcatagg gtcgatgatc gctgatatgc tcaaattacc actccatgag cgaagaatgg
    14821 ctcttaatat tggaataggg gcaggaattg gcacaatttt cctggcacct cttggcggcg
    14881 ctatccttgc tgctgagatc ctttatcgaa gagactttga atcagaagtt atcgtccccg
    14941 cgttcctcgc ctctatcatc ggctattcaa tattcggact cttcgagggg ttcagtccgg
    15001 tgtttgcaga agctccgatt gtatggaacg tgtttcagat tccatttttc cttgtccttg
    15061 gcctgatatg cgccgggtat gggtggctat acattcatac cttttacagg agccatgagg
    15121 tatttgcaga ccttgtgagg aagtatcatt ttccaccgtt tataaagccc gttataggcg
    15181 cttttcttac cggtctaatc gtcattatcc tctttctctt catccccaac ggtgcagaaa
    15241 tcggcctggg cagcctgggg tcaggatatg ggttcgtcca gcttgcgctg tattcgatgc
    15301 ttcccattgg ggttctcgtg gccctctcgt tcctgaagat tcttacgacc tcactcacca
    15361 ttggttcagg tggcagcggg ggtgtctttg caccgggtct cgtgattggg ggaatggttg
    15421 ggggaatgtg cggagtgata ttcaacatcc ttttcccggc ctatttcccg gtcgagacca
    15481 tccctgcatt tgtcataatc gggatgatcg cactctttgg atgcgtatca tatgcgccca
    15541 tcgcggtgat gatcatggtc gtcgagatga ccggaaactt ttctttgctg gtccccgcaa
    15601 tgggcgcagt cgcaatcgca tgtctgttca cgaggggcag cgggatatac cgcgcacagg
    15661 ttgagacaaa agcagattcc ggggcccatc gcggagaata cgaggtcagc atccttcaaa
    15721 aaatccctgc agggaccagc atgactccgg ctgagaaggt ccttgtactt catcccgagg
    15781 ataagagcag ggaagtcctt catctcatcc gtttacacgg gcataacgga tttccggtca
    15841 tcgataatgg gaagctggtc gggatcatga ccatccagga cgtgcggcag gtaaggaaaa
    15901 agcaggacct ggatgcacca gtgaaggatg gaatgagcag ggatcttttt actctcacgc
    15961 cagataccac gcttgataac gcacttaaga tcatgattga gcgtgatatc aatcatattc
    16021 cgattgttga tacgattgaa tcacagaaac tggttggttt cctgacccgc acggatatca
    16081 tgaatgcgta tgccaggtat tcggaagaga attcgctttg atgcggggat tattgcagat
    16141 taccggatac cttcattaca cctttttgta atttttttta gaaaggtgtc attgtaacta
    16201 ataaggacgt ccaaccagag agtaagtcta agagaatatt catgaaccgg atcatccatt
    16261 ttgaaattca ggctgatgaa cctgaacagg ttgcgaagtt ctacagatcc gtattcgggt
    16321 gggagatcaa cgaatgggtt cttcccggat ttgagatgaa ggacgaggac cggtactgga
    16381 ttgtgatcac cggggctgaa ggcgaacccg gcatcaacgg cggcctcgta ttccgccatg
    16441 gacccagggc tcccggcgga ttgtcggcga acgcattctt ctgcacgatc ggtgttgccg
    16501 acctcgacaa gtccgtcaag aaagtgaccg atgccggagg aaaaatcgtc gtccccagga
    16561 tggcaatcca gggagtgggc tggtgggtgt cctgcctgga ccctgaagga aaccagttcg
    16621 gcatgatgca ggaagacccg gatgctggat agcgtgccgt gctatccatg tccatccctg
    16681 gatagtccca gtggatacgg ctcctttcta ttttttgaac aggacaccgg gaggatcagg
    16741 acagaattcc atgcggtgaa ttcagcagat acacttccgc agattcatcg gatccccgat
    16801 ggacgataga tgaactcttc agagatcctc agtctccgtc tttccaattc cggattaagc
    16861 cattctccgt tccaaagccc aacggatgct gtcacccagc ttgcggccgt ccaggcccag
    16921 gatttcactg ccgcaaaatg gtccctggga cttcgcgtaa aaaaatccac cgatgctgac
    16981 atcgaacggg ctttcaacga aggcagaatt cagcgtatcc acgtaatgcg tcctacctgg
    17041 catttcgtca tgccggagga tatccggtgg atgctcgaac ttacggctcg acgggtgaaa
    17101 tctcttctcg cggcttccaa ccggaggata ggtctcgatg aggcgttgct tgcaaagagt
    17161 aatgccgcca tcgtgaaagc cctggaaggt caccgctatc ttacacggca ggaaatgaaa
    17221 acggtcctga atgatatcgg gatcaggacc gatgtccggc aactggctca tgccattatg
    17281 caagccgagt tggacggatt gatctgcagc gggccaaagc gcggcaaaca attcacatac
    17341 gctcttctcg acgagagggt caggaaatca gcaaggatca gccgggaaga agccctgaaa
    17401 aaaatcacac tcagatactt taccagccac ggacccgcac agcagaagga tttctcctgg
    17461 tggtcagggc ttgcagagaa ggatgcagaa aacgcaattg aattgattgg atcccatctg
    17521 gatcggaaga tcgtgaacgg aaaagaatac tggttcgctc cacgggaacc gggggagatc
    17581 cccgatcctc ccactgcatt gctgctttca gtatacgatg aatatacgat cgcgtacaag
    17641 gacagaagcg atctcagcga agcacgggaa atcgagcgga tgattgccag ggggaactct
    17701 tttcccgcag tcctcatact cgacgggaaa gtggcgggca catggaataa acgcatgaag
    17761 agccaggccc ttgagatccg tctggatcct ttccgaatcc tggatgatga agagcaggag
    17821 gcggtggagg cagaagcctc ccggtacgga aggttttttg gagttcctgt gatattcgtg
    17881 atggaaccat ccggtcggta gatcgctgcc atcctgagtg ttgatccggg ttgaaccaat
    17941 gatccgcagg ttctgttccg tccttacaca tagtgtccgg gatcggacag cttccctttc
    18001 tccgtatctg tcacacacac cgggcactcc gtaacacaca tgctgcatgg catggcagca
    18061 tacctggcaa tccacggcac cagcggtccg acgaaacgaa gaagcagtgc ccggcggagc
    18121 atccatttcg cagcagggac aaggcacccg ggaatccatg cactcaacga ctggcacctg
    18181 aacgcatcga caccatcccc accaatcgca tgtccggggc atgcacgaat gcaccgctcg
    18241 cacccggtgc acaacgagga ctctggtctt ttgaatcctc cggttttttg ggctggtcgt
    18301 gaggtgacga ctccgcccag tatcacacgc gggccgaact ggggagtgag gaggagggta
    18361 ctctttccga tcgatccaag tccccccgct tctgcgatct gcttcaggcg gacgagcccc
    18421 ataagccggc cctcaacgat cctgatcggg aacaagagcg gaacaggccg tgccgcactc
    18481 tgttctgcaa aaaagaggcg tgttagattc aatgcagcct gttctaccga ttccgcgatc
    18541 tggaacatcc cgcgtgtctt ttctcctgcg ggcatccggt acacatccat aggcacctcc
    18601 tttccgaaaa caatcacgga cagtgcatcg ggaaaaaatt cacgtattga ggattgatcg
    18661 tctatggaaa gcgcatccag tccgacttcc gaaaagacat cgataccgtg ctcccggaag
    18721 aattgttcaa gatccaccac ggtattccct gaaagatcat ggatgcagga tacatcttga
    18781 taatgtcggt gtttgaagcg caggaatcaa agcctgcaaa aatatttggg gtgagggctg
    18841 ctctatctgt tcaatgaacg atacgaaaat catcgatctc acccccgaca ccatcgcgga
    18901 ttacggcgtc tgcggttata aagacgcgcg aaaacacgtg gagctgcaga gaaaaatcgc
    18961 gtggttcaaa aaatattaca cgaaaggcct gaggataaag gcactcgtgt cagaaacagg
    19021 tgggtaccag ggaatgctcg aatacatgcc cggggagtat gcccaccgcc ctgtcgatgc
    19081 ggacgggtac atgttcatcc agtgcatttt tgtggggttc cgcaatgaat tcaagggaaa
    19141 aggctatgcg tcatccatga ttgatgcctg cataaaggag gcaagggatg ctcatatgaa
    19201 gggcgttgcc gtcgtgacaa ggaaaggctc cttcatggca aaacgggata ttttcctcaa
    19261 atacggattt tctccggcag atacggcgga acctgatttt gaactgttgg tcctgaaatt
    19321 tgacccggat accgcgaatc cccggttcaa accaaccctg aagaaaaacc gcgacctgta
    19381 tgcagacggc ctgactatct ttcgttcgcc ccagtgcccc tattccgaga agaatgtaaa
    19441 agccatcatg gagactgcac ggaaaaagta tgggctttct ccccggttgg ttgaattgga
    19501 agatgcagaa tccgtccaga attccccatg tgcgtttggg actttctgca ttgtgtataa
    19561 cggggaagtc atcagtcatc atccgatcag caacggaagg tttgtcaata tcatggataa
    19621 gatcaggaaa tgatgtttca gggggagaat cgatgaatgc gactatccgc ggacgctgcg
    19681 gatttctctg cagtaactgc cccgcttaca aggagaacat tcactccgat tccgaccgtg
    19741 aacgggtgag tgtcgtgttc cagaaacttt atgggcatat tatcgagccc gaaggcgttt
    19801 attgcgacgg ttgtctcgaa cctgacgaaa ataacatcag gcggcttggg accgaacgct
    19861 gccccatgcg ggattgcgtg ctcgacaaga atatccccca ctgcggtaag tgcagcgtgt
    19921 ttccctgcga gctgatggag aggcacctgg caaccgttga gacagttgtc accaaagccc
    19981 gcacaatgct gacgccggag gagtaccggg atttcgtgga gccgtatctg tcgcgggaat
    20041 tcctgtcggg aaaaaccgga taaaaataaa aatagtctga ttaaaaatcc agggaataga
    20101 tcaaggggct ccctgaaaat tgataatcaa tgaattaata catacaccac gccaaccaat
    20161 gtgacaggga taacatggat gtcctcactc tgctccggca gcacgaaccg gaactgaaga
    20221 gtcgattcgg tgtcgcaaag ataggcatat tcggctctta tgcgcgcgga gaggaacggc
    20281 tggacagcga tgtggatgtt ctcgtcatct ttcagaaggg aaaaaaaacc tttgacaatt
    20341 tcatggggac caaattctat cttgaagacc ttttccgtcg gagagttgac ctcgttacgg
    20401 aagcagcgct gaaacctctc atccgcgatc ctattctgaa ggacgtcctg tatgcctagg
    20461 tcccaactcc tctatctcaa tgatattttt gaggcgatac agaatatccg gttgtatgtc
    20521 ggggcactca cattggggga gtttcaaaac gatcagatgc gggtagacgc agttgtccgg
    20581 aatttcgagg ttatcgggga ggctgtcaaa aatttgagtg atgaactgaa gacccagttc
    20641 ccagatacag actggaaagc cgttgccggg ttccgagata ccctgatcca tggttatttc
    20701 ggcatagacc tcgagatcct ctgggatatt atttcaaata agatcccggt gttggagaaa
    20761 gaggtagccg atatgatcgc atacgagaac gcgaagaatt aaatcaaaaa tcaattttca
    20821 tttttttccc tttagggtat ttggtatcag aatttttcga tcgggatcct aaatatcagt
    20881 tacacctggg ggaaaagtgc agagaacttc tctagaaaga ttatttccca tgcaagatgt
    20941 gtgattcgca ccacgtttcg gtgtgaactg acaaaaaggc actatatata cattgaatcg
    21001 gagatctcca aagcccacga gatgatgatg ctagaggaat accaagattt cgtgaagcca
    21061 tatttaagtt ggaagtgcct gtcaggaaac gatttgaaat gaaagtattt gataattgaa
    21121 aatctccctg atattctgta gtctaatgaa aaagtataac aaaacgaata tacaagcagg
    21181 attttaaaga gagaactatg gggatttttg acatctttaa aaacaagaaa caagtaaacg
    21241 atgaaaaagt ccaaatcatt ccccaaaata aatcaaatat caaaatcaat tcatctcatt
    21301 cactcgtgcc gtattttggg aacttgataa ttcctgaaaa tatccgtcat ttgctttggt
    21361 ttggtgatgg aatatttcaa aattataatc cagataatga ccatagagga tttcgaaaaa
    21421 cccgcttcga actgcaagag ctttccctta gctgatcgaa gtattctcaa tgacctttga
    21481 gacatttgga ttaaacgagg catataagca ggtacaaaag ggaggagacc atctgtcaga
    21541 atttgcaact ttgatcgatt gggatgtatt tcttcccata ttcgagggac tctagcacaa
    21601 caaaacaagc aaaggaggaa ggccgaacgt cgatccaatt gtgatgatca aattattggc
    21661 actccagtct ctctacaatc tctctgatcc tgaactggaa cgccaggtga cagcccgcat
    21721 ctcttttcgg aaatttatcg gattcacgac tgaaataccc aatttctcga cagtctggct
    21781 ctttcgggaa cgtgtttatt gaatcaggga aatacgagga agtcaggaac acattccgaa
    21841 cccaaatcga tctcaagggc ctaaagataa catgaggagt cattcaggat gcaactctca
    21901 ttactgcgga tcccggaacc acctcatcag aggtgtcaca ggggaaagga gggaagacgc
    21961 ggagaagcaa ggagaatacc tggacaaaaa gaggacagcc accatacttt ggatacacgc
    22021 ttcacgcgct aattgacaaa gaatgccatc tgatccgacg catcgagaca accactgcac
    22081 cggtccatga tagccaagga aacctctcga tgcctggtga gactgtgtat cgggataagg
    22141 ggtatttcgg agtgaaaccc tgtgcggcaa tggacaaaac catgaaacga gcggtccggg
    22201 gtcacccact caccatcaag gagaaacgca ggaatcgcgc aatcagcaga gtgagaaggg
    22261 tgattgaacg ttcatttgcc gttataaaac gaaccttcca ttgagatcat gtccttgtca
    22321 cgactattga gagagcatat gtcaaaaact tgttttcatc attttcgctc aatctagtcc
    22381 agttaggcac gctcaaacgg cggggggtct ggtagcggta gctatccaag attttagaga
    22441 aaacac
//
LOCUS       DAOQYR010000027        22078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_2713_length_22078_cov_9.028289, whole genome shotgun sequence.
ACCESSION   DAOQYR010000027 DAOQYR010000000
VERSION     DAOQYR010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 22078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22078
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2713_length_22078_cov_9.028289"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PKM87_04930"
     CDS             complement(<1..566)
                     /locus_tag="PKM87_04930"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ29446.1"
                     /translation="MTSRDDPGKHHQHSVFSASHAAHLDTRFRRILYRPDHLSERYVK
                     QGDRVLDFGCGPGFFTREFAKRAGDTGRVYAVDLQDEMLRIVEGKMESEGLLPRVTTH
                     QCRPDSIGLPPELNDTFDVAFAIFVVHEVPDPEKLFREIAALLKPGGTFFFSEPPIIV
                     PGREFEDKVRLAGEAGFRPVDRSWFFVNR"
     gene            863..3082
                     /locus_tag="PKM87_04935"
     CDS             863..3082
                     /locus_tag="PKM87_04935"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HNQ29447.1"
                     /translation="MKTISAFIVASVLICSFAAGVHGETTTEGSDGSLITLQGNTITL
                     EGSGAKIGASTVTITSGGTYTICGTLNNGQVIVDCPDTEKVTLVLSGAHISCATSAPI
                     YVASADKAVITLADGTSNSVTDGSSYVFPDAASDEPNAAIFSKDDLTINGGGALSVTG
                     NYNDGITSKDDLKITGGSVTVNAKNDGVRGRDSVVVREGTLTITASGDGIQSNNDEDA
                     EEGYVSIESGTISIIAGADGIQAESTLAISGGSITITSGGGAGNGNTATPRDNQGRST
                     ITVESDTGSAKGLKAGAALEVSGGSITINSADDAIHSNGSITIDGGTMTLASGDDAIH
                     ADSSIETNGGYIDITNCYEGLESEIITLNEGTIHLTSRDDGINAVSKTAVAVTARQPG
                     QQPFEASGNCALGIHGGYLVVNAGGDGFDINGPVTMTGGTVIINGPTDNGNGAIDYLG
                     SFTTSGGTLLAVGSSGMAQAPGTSSSQNSVMVTYSSLQQAGTLVHIETSDGDNVLTFR
                     PAKAYQSVVFSSPSLKRSTEYVVYSGGSTSGTSSDGLYFGGIYTPGTEVARFTISGTV
                     TMIGSSTAQGPGNLPGNRNQPMPGNTANNSTGTRDTAVPGSWGTAMPGNISGTMNGMI
                     SNARRSTVPGASGPMNGMIPGSWGTMVPGNASNPRDGVVPGTWATRVPGMPGTANGME
                     SIPGDTFTYRVYSPVPRITPDMGTTSFPTTHSGTFSSSFSTSTSGGVTYSRPSGIRV"
     gene            complement(3300..4094)
                     /locus_tag="PKM87_04940"
     CDS             complement(3300..4094)
                     /locus_tag="PKM87_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29448.1"
                     /translation="MNRISKKTVLTSLLIAAVIALVAWVGSVAFFFHQSMAESERHDF
                     HYSISLSYTTTITNVTILLPVPSLNGTPVLADAFVQRSAPGIPGDWNLSIEEVNGTPM
                     LAIRADKMVPEYHGYPIPIEPGQSPLPATIAPRTEYSLETPVLRPIQIGTTLQVNRTI
                     ETRDPVGREPVFAQTGEFVLRKENAGPHTQGLEFVHPVPVYVWFEREKPGEFSLQASI
                     QGTNSIWRGGWVFNSYQDSVSVELKDPPGWTEAVGILRTGEGAYYT"
     gene            4283..5098
                     /locus_tag="PKM87_04945"
     CDS             4283..5098
                     /locus_tag="PKM87_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29449.1"
                     /translation="MTVSKEQGHVYAGIVILVLLVLGVTLGAGCLEDPAHETLGLQNE
                     AAIAIALRDTIVREQIPIESGEYEIVDVGPEFCMQTGPGSTFSRTCTAVTFRCSSQRS
                     LYHVIIDDADETVVGRYWQWVKEPMPCSGGEPPVEYSTIEEASIAVKPGCPLATLPVI
                     PAAYSFSIVRIYGEPCPRRDIVYTRGSEELRLIHVCPGHPPYAFAITGKGADEVAIRG
                     SPGKFVQGIGQNQLAWADAHGSYWLLGDMSREDLVAVASSVEPYSRAGGTVSG"
     gene            complement(5173..6642)
                     /locus_tag="PKM87_04950"
     CDS             complement(5173..6642)
                     /locus_tag="PKM87_04950"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HNQ29450.1"
                     /translation="MEEKKKLTTAAGAPVADNQNAVTAGPRGPMLMQDVWFQEKLAHF
                     DREVIPERRMHAKGSGAFGTFTVTHDITRYTKAKIFSKIGKKTEVFARFSTVAGERGA
                     ADAERDIRGFALKFYTEEGNWDLVGNNTPVFFLRDAHKFPDLNRAVKRDPHTNMRSAK
                     NNWDFWTSLPEALHQVTITMSDRGIPLSYRHMHGFGSHTFSMVNAKNERVWVKFHFVT
                     QQGIKNLTDEEAEAIIAKDRESHQRDLLTSIEKGDFPRWKMYIQVMTEEQATQMPYNP
                     FDLTKVWYKKEYPLIEVGVLELNRNPDNYFQDVEQAAFNPAAVVPGIGFSPDKMLQGR
                     LFSYGDAQRYRLGVNHHQIPVNRPKSAPAHPSHRDGQMRVDGNYGATIAYEPNSYGEW
                     QEQPGYREPPLELSGAAAAWNFREDDSDYYTQPGKLFRLMSPAQQKVLFENTARAMGD
                     APEFIKIRHIQNCLKADPAYGKGVADACGIPMDAVMKSS"
     gene            6797..7579
                     /locus_tag="PKM87_04955"
     CDS             6797..7579
                     /locus_tag="PKM87_04955"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HNQ29451.1"
                     /translation="MMPPGITDRVLVVEGDITRQRVDAIVNAANPSLLGGGGVDGAIH
                     RAAGPELLGECRDLGGCNTGEAKITSGYRLPARFVIHTVGPVWKGGGAGEDTLLSSCY
                     QNALALAELIGLRSIAFPAISTGAYGFPLERAARIAVCTIIGFLTYHPAIERVVLACH
                     GNAAHTLYQDILGQEKTINTEYEMAQALFSHISMIEASHGIRIKNREAIARIIAPTIK
                     SDTQALSVGISLNNWVAVAGAYGEVTVSESVIRTILDSLKSA"
     gene            7806..8696
                     /locus_tag="PKM87_04960"
     CDS             7806..8696
                     /locus_tag="PKM87_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HNQ29452.1"
                     /translation="MPGLLLLTGYFAVMVFIVTSMLAMGLNISICDLISPLRNRRLVI
                     LSLLANFVIVPLFAFVLIYVFPVSRDLAAGLILVSIAAGAPSTPKVAELTEGNIAYAV
                     SLTLLMTILTIILMPILVPYIMEGMGMNPAKVAFNLVIFMLLPILVGMLLRNRAPERA
                     QKVLPLAELVSNASIGVIFLAFGIIFFAHVVDLFGHPGGFEIAAIAVLFTVGALLIGY
                     FMGGPERGHRDVLAFSTGFRNITAALVVITASYSDLDNDVFLMVLMITLFSVIIVSAI
                     VGIIIKKRIDAGKQSRLQPS"
     gene            8783..9382
                     /locus_tag="PKM87_04965"
     CDS             8783..9382
                     /locus_tag="PKM87_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29453.1"
                     /translation="MARSLTIYLAYFFQALIALNGIYALVTGQYQAMFTAFLMFGLTL
                     VPYIVAQRMHINLPWFVYLLIALALWIHTAGYIQGYYTLFYPYYDKVAHLISGTAVAL
                     LGFLGVIFLDKYWKMTLTTPFIIAFTIIFSVALGAFWEMYEFFVDMVFGGSLAGPMQN
                     GLNDTMFDMIFVLAGSIIVAILGHSYLKKNPKEKITGDA"
     gene            complement(9506..10411)
                     /locus_tag="PKM87_04970"
     CDS             complement(9506..10411)
                     /locus_tag="PKM87_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ29454.1"
                     /translation="MSRWSFLLPTRYFAISAVLLVVAGAGAGYAYWEGAHPQVTVLSI
                     DGAPEGIVFIADPHVKPGNIEKVRKVIQTINELNPSIVLIGGDFLFGEEDNLSCQEIW
                     SEIDAPVYAVLGNHDYHAGIDATSAIAKSIAVSRTDRDLLTYDMSGLFDETTNLASAA
                     ALEEALEAHGVMVQRNEYVMREVSGTPLLLVGVDDGWAGMADPPAVPATDAFSIYMIH
                     EPECRADWDADLILSGHTHGGQFGGTALQFINDAGIMELSGYVRDSRVPVYITRGIGT
                     SNLPWDLRIGATPEIVIINPASPVQ"
     gene            complement(10503..11750)
                     /locus_tag="PKM87_04975"
     CDS             complement(10503..11750)
                     /locus_tag="PKM87_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29455.1"
                     /translation="MQKTARMIYPVLAAMLLLFAVFSGCVTEIPDSCNLTEGELADRY
                     LAQAATIRDLQSEYVVYSGMAAGDLRVEKISFYFKTPSFTRIEMEPETAGEQGSFATS
                     SGTSTSWYDSRTRVYDLYSGMNLSREYDYQRMVRHIIADRDFAILERDTEHGASRYLI
                     EVVTDPWSDRYTPYISSRVRARVEPSTGLAWEVATYYDSSVAGQPTLAPGMVLPTPTP
                     PPIDMAPPGTIDRSELPNREVRYESIAVNSGIPDSYFEFVPPEGSSPRCIPKYVNYVE
                     PPRTDPSVPISEPLPGRVRYSLNETDSGRVVTLERGEILEITLRIIPSLATRWLMPAE
                     GSGLELLNAGAFSVLPDDLDLNSSGAMVYFKSNGYYRWRFLAKDPGTTTFDGIFSLDG
                     CDIGGAKRFNLTVRVIDNTSPAL"
     gene            12030..12218
                     /locus_tag="PKM87_04980"
     CDS             12030..12218
                     /locus_tag="PKM87_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29456.1"
                     /translation="MIKNSARPTVIGTRSGYTIRFTCPNCAKENSIVYNMPKAFYKES
                     RDAVCGKCRKHFTVLTPD"
     gene            complement(12318..13832)
                     /locus_tag="PKM87_04985"
     CDS             complement(12318..13832)
                     /locus_tag="PKM87_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNQ29457.1"
                     /translation="MNRILSYSLIVLFLIAAATLAAGCISEKDAGASSTVDEPAAKAA
                     AEYEASNYRSAAELYMQAYSDYQARGDTVSARKAMREALVSQRMIFEFPFNRSAVESE
                     IIQAYPEFSAREIADLLSEEKSIRIVSDGEHLYFSDTVSNIMFHNLTLARRMTKEFNH
                     SPFYDETCSLAFATPLPNKGPYGEPVVFEITQRLLLPTVLLPETGTMRIWVPAPIESG
                     SQSDVGILSIEPAQYVRSWPDTSADIGLAYLEIPLADVKGDLLNITTRFRFTQHEVRF
                     VIDPSRVKAYNLSNPEYLQYTMSEKNIAVTQEIKKKAHEIVGDETNPHLQAEKIYWHI
                     MNTLPYSHVPHFMLETAGIPESTYVLETGFGDCGSQSMYFAALCRSLGIPARSTGGFQ
                     LVPGLAGEHFWAEYYLEGYGWIPVDVTVAESADWSHDATDEDRHRYKEYYFGSLDPYR
                     YIIQKNVDAPLTPDPGDAVMFRLAVQNPKIACDTCTTDMEILLVEYWTTNVTQG"
     gene            14018..14347
                     /locus_tag="PKM87_04990"
     CDS             14018..14347
                     /locus_tag="PKM87_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HNQ29458.1"
                     /translation="MKVGIIRCLMTEDLCPGTMDFRVTAEGKGAFADTGPVEIVGFVT
                     CGGCPGKRAIPRAKMLVERGAQAIVFASCITRGNPIGYPCPHYADMRDCVKKAIGPDI
                     SIIEWTH"
     gene            complement(14363..14674)
                     /locus_tag="PKM87_04995"
     CDS             complement(14363..14674)
                     /locus_tag="PKM87_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNQ29459.1"
                     /translation="MAKIAAELVRDAADLLGIRDQASLHEIRALYHDRVREWHPDVSR
                     KDPVESHEMTIRLREAYELLVEYCMNHPFSFRAEDLARELADAAADYWMERFGDDPIW
                     G"
     gene            14779..15948
                     /locus_tag="PKM87_05000"
     CDS             14779..15948
                     /locus_tag="PKM87_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNQ29460.1"
                     /translation="MAEDRPLIYLNNAATSWPKPPEVFAAVLESLSLPVFGSGRTTVS
                     QGEDYVTKARDALSGLFGVETAEHVVFTQNATDSLNVLIQGFLERHAGDCHVLTTALD
                     HNSVLRPLHEYERQGRIRLTVLPFEDGRVSPAAVTEAITPMTRLMVTTHGSNVLGTAA
                     DIRGIGEVLHDHGAYFVVDGAQAAGHVPIDFESLPVDAYAFTGHKALLGIAGTGGFVI
                     KDPEAIAPVRFGGTGTDSFSLHHPAGMPERFEAGTHNYPGLAGLAAGVSFILSTGIET
                     IMEKAERQTAYLVGELAKEHNIRIYQDRPDLPIIAFNIRNLPNDDIGFILARRYGIIV
                     RTGLHCAPLVHRAIDGGEGCVRLSHSWFTTDEECRIAAGAVREIAQNADRPVSSA"
     gene            15920..16288
                     /locus_tag="PKM87_05005"
     CDS             15920..16288
                     /locus_tag="PKM87_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29461.1"
                     /translation="MRIVRSVRHKVCADGSFMKEFLVDEPIPASFFSFLTNFGKVEAL
                     PQIGEGFFRFEKPDWFSIKGFAGDTTVEVRFKKEVMDLTMDFAYSLFSSFQNEKPDLS
                     GLKQREEAVAGRVRRRLHGE"
     gene            16303..16875
                     /locus_tag="PKM87_05010"
     CDS             16303..16875
                     /locus_tag="PKM87_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ29462.1"
                     /translation="MPEDVFERYAEDYDLWFEEHPAEYRSELGRVRALSPPIAPFSLE
                     VGAGSGRFAAPLGIGLGVEPSVALCRMARWRGIEVVRAIGEAIPFRDASIPSVLMVTV
                     ICFLEDPSRVLAELQRILVPGGAVVIAFIERGGPIHQKYLYEGGKGRFLSCARFYTKE
                     EVLSLLENNGFWVTAVDPRAGFCVIAAQKG"
     gene            17047..19389
                     /locus_tag="PKM87_05015"
     CDS             17047..19389
                     /locus_tag="PKM87_05015"
                     /inference="COORDINATES: protein motif:HMM:NF040095.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HNQ29463.1"
                     /translation="MKSVTMREGEVLVLSLEGRLDAQGAVEIDAVLKDLLRETDSTII
                     FDMSGVTYMSSAGIRTIVATEKRMKERGGRIHLSCLQPYPRSVLDMTGFSTVLSIHST
                     RKEAVRAARATVGREIGVPGHAPLAVQTRGAEFVATCTGQDACTLDITGFPTDERSGG
                     RDTGPAIPVTIPVSACSLGFGSPGLPDDDEESAMGDFLSVGHIAAWVLPDSGDTLDYL
                     VLEKNVARIPLATSFLISPSAPPGCEVRVHAGSPGGITLSDLFDSLHEIAKEMDPRYC
                     GVLCTSFCAESPDVQTLNPAEGTPPTATLAGCAVIVDAAALPDHLGGVVTDALVRHLP
                     GRPGIVPRATALVFRDLAAGEGESACEAVARGLSSDVQALLRHLSPRTRIFQATLQLF
                     VISDIRLHTGTTIVFKGDVPGWNPDYERITKSVHHDCSEVRLHPISGGYSGSLVFRDD
                     AYDRSGRREMPFVLKLDRWENIRAEIEGYEGHVKRYIQNNATQIIQKARSGEYGGILY
                     TFVGIQGPQSRIFSLEDYYRTHPTDEVLAVFEILFRRVLRAWYGQPRLRDLPLYDVYS
                     DIFRYEDVRRWAESRYSITAADETIDLPFGLGRSANPLHFMEHILPVRRSRTWSVYEG
                     SVHGDLNMKNVLMDDEQNLWLIDFAMTGHSHILRDVAKLESVLKLEMIPIESQERLFE
                     LVALEQVFLTPKKLGEIPSLPEGIADPDIAKAFQVVHQLRRYADTITLLDEDILQYYL
                     ALLYYTLCVPAFVSVNDYMREYAWISSSLLCEALRIHGEH"
     gene            19752..20234
                     /locus_tag="PKM87_05020"
     CDS             19752..20234
                     /locus_tag="PKM87_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HNQ29464.1"
                     /translation="MDIVNAFVGLIQLVIAIILAVAALYIGYATFSKITKGMDEAAEL
                     RKGNVAVGIVIASILFAIAIVVQSGIAGISIGIMNALAGDWFSLIAAVIQLILGIILA
                     VAVSYFALHVMDRLTKGLNEFEELKKGNVAVALLMAGVIIATAVIIQSGVIGITSALI
                     "
     gene            complement(20481..21179)
                     /locus_tag="PKM87_05025"
     CDS             complement(20481..21179)
                     /locus_tag="PKM87_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29465.1"
                     /translation="MATTDIATQTMTSLGDSLTQMFSSVVAYIPYLIAAIIILLIGWV
                     VGRVLGSLVSKILDKVGVDDALRKTSLGKAIEGTGMHIVRLFDLIVRWFIYLIAIAAA
                     ASVLQIGFISELFEKIILYMPHVAMFLIILVAGFVIVDYFSDLMHAWGKTQNIEFLGV
                     IIMLLRVFFYFVVLILALSQLLIDLTIIYTFITPIAWGVGIGVGVGVAVFLAFGLKDR
                     APEMIDNMLKKIIK"
     gene            complement(21542..>22078)
                     /locus_tag="PKM87_05030"
     CDS             complement(21542..>22078)
                     /locus_tag="PKM87_05030"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HNQ29466.1"
                     /translation="GDNLSMISKSVGSDEKSVQSALGMGLPMIMGSMAQTSQKPGGAD
                     MITSMMGQMGGSNPLDNLGGFLGSSAASGGSGMASSLLGSQMAPISNAIAQKTGLPSA
                     VVEKILAIATPMVMGYVTKSMGGKQMDQQGLTSLLGEQSKMAMQSSPDAARMAEQMLG
                     SQKEAAGVSGIFKKFLGK"
BASE COUNT         4651 a         6318 c         6339 g         4770 t
ORIGIN      
        1 cggttgacaa agaaccagga gcgatcgacc ggccggaacc cggcctctcc cgcaagccgg
       61 accttgtcct caaactccct gcccgggacg attatgggcg gctccgagaa gaagaatgtc
      121 ccgcccggct tcaaaagtgc agcgatctcc cggaagagtt tctccgggtc aggaacctcg
      181 tggaccacga agatggcaaa cgccacgtcg aaggtgtcgt tcagttcagg aggaaggcca
      241 atcgaatccg gcctgcactg gtgcgtcgtg acccttggga ggaggccttc agactccatc
      301 ttgccctcaa cgatcctgag catctcgtcc tgcagatcga cagcgtagac ccggcctgta
      361 tcgcctgccc gtttcgcaaa ttcgcgggtg aagaaccccg ggccgcaccc gaaatcgagc
      421 acccggtcac cctgcttgac gtatcgttcc gagagatggt cagggcggta gagaatgcga
      481 cggaaccggg tatcaaggtg tgcagcatgg gatgcggaga atacactgtg ctggtggtgt
      541 ttcccggggt catcgcggga agtcatgcga ggagtatgtg cgcagcgtcc cgataacagt
      601 tctgtaataa ttgtgggatc ggttttcctc ccaacgaggg gtgatgaacg cttctgccgg
      661 agagggcggc ctgcctcttt cccgctgcta tgcggggtac tggattgaag attcttggca
      721 aaagatattt acacaatgta ttgtatactt tacattgtca aatgagcggg tcttccccac
      781 gcgaaggaaa tatcctctgc gggataggag gcctgcagag taaattcaag aaaataccca
      841 gaaaaacagg agagattcct ctatgaagac catatctgca ttcatcgttg catccgtcct
      901 gatctgctcg tttgccgcag gcgtgcatgg cgagacgacg acagaaggct ccgatggatc
      961 cctgatcaca ttgcagggca acacaatcac actcgaagga agcggtgcga aaattggagc
     1021 ctcgacggtc accatcactt ccggcggaac ctacacgata tgcggcactc tgaacaatgg
     1081 acaggtcatt gtcgattgcc ccgatacgga gaaagtcacc cttgtccttt ccggagctca
     1141 tatctcctgc gcaacaagcg cccctattta cgtggccagt gccgacaagg ctgtgattac
     1201 ccttgcggac ggaacttcca attcggtgac ggacggaagt tcttatgtct tcccggatgc
     1261 cgcttcggat gaaccgaacg ctgccatctt ctcgaaagac gacctcacca tcaacggtgg
     1321 cggagccctc tcggtcaccg ggaactacaa tgacggcatt accagcaaag acgacctgaa
     1381 gataaccggc gggagcgtca cggtcaatgc caagaacgac ggtgtccgcg gcagggactc
     1441 ggtggttgtc agggaaggga cccttacaat caccgcgagc ggtgacggga tacagtccaa
     1501 taacgacgag gacgccgagg agggctacgt ctctatcgag agtggcacca tctctatcat
     1561 tgctggggcg gacggtatcc aggcagagtc taccctcgcg atcagcgggg gcagtatcac
     1621 gataacatcc ggaggaggtg caggaaacgg taacaccgca actccccggg acaaccaggg
     1681 caggagcacc attactgtcg agagcgatac gggcagcgcg aaagggctga aagcaggggc
     1741 agcgttggaa gtgagcggag gttcgatcac catcaactcg gccgacgacg cgatccactc
     1801 caacggcagc atcacgatcg atggtggcac aatgaccctt gcctccggtg acgatgctat
     1861 ccatgcggac tcctcgatag agaccaatgg cgggtatatc gatatcacga actgctacga
     1921 ggggctggag agcgagatca tcacacttaa tgaggggacg attcacctca catcccgcga
     1981 tgacgggatc aacgccgtat cgaagactgc tgttgccgtg acggcgaggc agcctggcca
     2041 gcagccgttc gaggcctccg ggaactgtgc cctgggcatt catggcggat atctcgtggt
     2101 taatgcagga ggagacgggt ttgatatcaa cggtcccgtc accatgaccg gcggcaccgt
     2161 gatcatcaac ggtccgacgg acaatggcaa cggcgccatc gattaccttg gaagtttcac
     2221 gacttccgga gggacactgc tcgccgtggg cagttcaggc atggcccagg ctccgggcac
     2281 ttcatcctcc cagaattcag tgatggtgac gtactcctcg ttgcagcagg ccggcaccct
     2341 tgtgcacatc gagacttcgg atggcgataa tgtactcacg ttcagaccgg caaaggcata
     2401 ccagtctgtc gtgttcagtt ctccctcact gaaacgcagc accgagtatg tcgtctattc
     2461 cggaggcagt acgagcggaa cttcatcaga cggcctctat ttcggcggta tatatactcc
     2521 cgggaccgaa gtcgctcggt tcacgatatc aggaacggtg acaatgattg gctcctcgac
     2581 tgcccaggga ccagggaacc tgccaggcaa caggaaccag ccgatgcctg gtaacactgc
     2641 caacaacagc acggggacaa gggatactgc tgtgcccggt tcatggggga ctgccatgcc
     2701 agggaatata tcgggtacta tgaatggcat gatttcaaac gccagaagaa gcactgtacc
     2761 tggtgcatct ggtcccatga acgggatgat tcccggttca tggggcacca tggtgcctgg
     2821 aaatgcatct aatcccaggg atggcgtagt gcccggcacc tgggcaacga gagtgccagg
     2881 tatgcctggc accgcgaacg ggatggagtc catccctggc gatactttta cctatagagt
     2941 gtattctcca gtcccccgta ttaccccgga tatgggaaca acttcttttc ctactaccca
     3001 ttcgggcaca ttttccagtt cattctccac cagtacatcc ggcggggtta cctattcccg
     3061 gccaagcggt attcgtgtat gaaagacaca tcactttttt ctctgaaatg tacatgtggg
     3121 gggatatcag ttgccatacc cctgggttac gccggtaacg caattccttc agcatttcca
     3181 agagtcgcct tcagttgagt cccataatct cctggcgata ttcgcaacct gaattctgaa
     3241 gattattcca ctacatatct aaaagataat agattgggga gccagcttgt gtcgatgtat
     3301 tatgtgtaat atgccccctc accggtccgg aggattccca ccgcctcggt ccatcccggt
     3361 gggtccttca gttccactga tacgctgtcc tggtaactgt tgaaaaccca tccacccctc
     3421 cagatcgagt tggtcccctg aatgctggcc tggagtgaga actctcctgg tttttcgcgc
     3481 tcgaaccaga catagaccgg cacggggtgc acaaactcca gcccctgggt gtgcggccct
     3541 gcattctctt tccttaggac gaattccccg gtctgcgcaa acacgggctc ccgcccgacc
     3601 gggtcgcggg tctcgatggt ccggttcacc tggagcgtcg tcccgatctg aattggccgg
     3661 agaacgggag tctctaaaga gtactcggtc cgtggcgcga tggtcgcggg aagcgggctc
     3721 tggcccggct cgatggggat cgggtaccca tggtattcgg ggaccatctt gtccgcacgg
     3781 atagcaagca tcggggttcc gttcacctct tcgatcgaga ggttccagtc acccggtatt
     3841 ccaggagcac tcctttgtac aaacgcatcg gcgagaaccg gcgtcccgtt gaggctgggg
     3901 actgggagca ggattgtcac gttggtgatc gtggtcgtgt atgagaggct gatggagtaa
     3961 tggaagtcgt gccgctcgct ctctgccatg ctctggtgga agaagaatgc gacagagccg
     4021 acccatgcga cgagagcgat gaccgctgcg atcagcagag aggtgaggac tgtctttttc
     4081 gaaatgcgat tcatgcgagt gtgtcctggc aacaatggac tggttctccg tgaaggagaa
     4141 aagcaatttc gatatgttcc caatgagagc aaaaagagca catccgagtc attttcaccc
     4201 gctgtatacc ccatgatggg accgcagtgc cgccgtcact ccaggtctca tttatcgtgc
     4261 ctgaaaagaa caatcccctg tagtgactgt cagtaaagag caagggcacg tgtatgcggg
     4321 cattgtcatt cttgtattgc tggttcttgg tgtgaccctt ggcgcggggt gcctggagga
     4381 cccggcacat gagactctgg gcctgcaaaa cgaggcagcc atcgcgatcg ccttgcgcga
     4441 caccattgtc agggagcaga tccccattga gagcggggaa tacgagatag tcgacgtggg
     4501 gcctgaattc tgcatgcaga ccggtccggg aagcaccttc tcacggacct gcacggcagt
     4561 aacattccgc tgctccagcc agagatcgct ctaccatgtc atcatcgatg atgcagatga
     4621 aaccgttgtg ggcagatact ggcagtgggt gaaagaaccc atgccgtgca gtggcgggga
     4681 acctccggtt gagtattcca caatcgaaga agcatccata gcagttaaac cagggtgccc
     4741 gctcgccact ctcccggtca ttccggccgc atactccttc tccatagtgc gtatctacgg
     4801 ggaaccctgt cccaggcgcg atatcgtgta tactcgcggc agtgaggagt tgcgactgat
     4861 tcatgtctgc cccgggcacc ccccgtatgc ctttgccatc acaggaaagg gggcagacga
     4921 agttgccatc cgcgggtctc ccggtaaatt tgtgcaggga atcgggcaga accagctcgc
     4981 gtgggctgat gcgcacgggt catactggct ccttggagac atgagcaggg aagacctcgt
     5041 ggcggtggca tcgtcggtcg agccgtattc ccgcgccggc ggcaccgttt ccggctgaca
     5101 cgacagggag tacaggagcg ggagggcgac aggccggctt ttaaaaaaac tgaaggaaat
     5161 aaaaaagcct cttcaggagg atttcatcac ggcgtccatc gggatgccac atgcatccgc
     5221 gacgcctttt ccgtaggcag ggtccgcctt caggcagttc tggatatgcc gtatcttgat
     5281 gaactccggc gcatctccca tggcccgtgc ggtgttctcg aagaggactt tctgctgggc
     5341 agggctcatc aggcggaaga gcttgccggg ctgggtgtag taatcggaat cgtcctccct
     5401 gaagttccag gcagccgccg caccggagag ctcaagcgga ggttcgcggt acccgggctg
     5461 ctcctgccac tcgccgtagc tgttcggttc atacgcgatt gttgcgccat agttcccatc
     5521 gacccgcatc tggccgtcgc ggtgcgaagg gtgtgccggt gcgcttttcg ggcggttgac
     5581 cgggatctgg tggtggttga ctccgaggcg gtaccgctgg gcatcgccat acgagaagag
     5641 gcggccctgc agcatcttgt cgggcgaaaa accgatgccc ggcacgactg cggcagggtt
     5701 gaacgctgcc tgctccacgt cctggaagta attgtcgggg ttccggttca gctcaagcac
     5761 ccccacctcg atcaggggat actccttctt gtaccacacc ttggtgaggt cgaaggggtt
     5821 gtagggcatc tgtgttgcct gctcctcggt catcacctgt atgtacatct tccagcgggg
     5881 gaagtcaccc ttctcgatgc tcgtgagcag gtcgcgctgg tggctctccc ggtcctttgc
     5941 gatgatcgcc tcggcttcct cgtcggtcag gttctttatc ccctgctggg tgacgaagtg
     6001 gaacttgacc cacacacgtt cgttctttgc attgaccatg ctgaacgtgt ggctgccaaa
     6061 cccgtgcatg tggcggtagg agagggggat tccgcggtcg ctcatggtga tggtcacctg
     6121 gtgcagagcc tcgggaagcg acgtccagaa gtcccagttg ttctttgcag aacgcatgtt
     6181 ggtgtggggg tcgcgcttga ccgcccggtt gagatcaggg aacttgtggg cgtcccgcag
     6241 gaagaagacc ggcgtgttgt tcccgacaag gtcccagttc ccctcttcgg tgtagaactt
     6301 gagggcaaag ccgcggatat cgcgctccgc gtcggctgcc cctcgttcgc cggcaacggt
     6361 tgagaatcgg gcgaagacct ctgtcttctt tccgattttg gagaagatct ttgccttcgt
     6421 ataccgggtg atgtcgtggg tgaccgtgaa tgtgccgaat gcgccggacc ccttcgcatg
     6481 catccgccgt tccgggatca cctcgcggtc gaagtgtgca agtttctcct ggaaccagac
     6541 atcctgcata agcatggggc cacgggggcc ggcagtgacg gcattctggt tgtcggcaac
     6601 aggtgctccc gcagcggtgg tcagtttctt cttttcttcc attatgtgcg ctcctttctt
     6661 ctctgtgccg gataagaggc gccatccggc cagagccgat agggtaatct tgttgcccac
     6721 cccgtatcaa ctttgccgac aggaacagtg ctgcattgag ccaaaaccca tatctctcca
     6781 tccatccccc ctctctatga tgccgccagg aatcacggac cgggtcttgg tggtggaagg
     6841 agacatcacc cgccagcggg tcgatgctat cgtgaatgcc gccaacccct cccttctcgg
     6901 cgggggaggc gtcgacgggg cgatccaccg tgccgccgga cccgaactgc tcggagaatg
     6961 ccgggatctc ggcgggtgca acacaggcga ggcgaagatc actagtggct atcgcctccc
     7021 ggcccgcttc gtgatccaca cggtcggccc tgtctggaag ggaggaggcg ctggtgaaga
     7081 tacactcctt tcgtcatgct atcaaaacgc ccttgccctt gcggaactga tcggcctccg
     7141 gagcatcgca ttccccgcca tcagcaccgg tgcctacggg ttcccgttgg agcgggcggc
     7201 tcgtatcgcg gtttgcacca tcatcgggtt cctcacttat caccccgcca tagagcgcgt
     7261 ggtgctggcc tgccacggga atgccgctca taccctctat caggatatcc ttgggcagga
     7321 gaagacgatc aacaccgagt atgaaatggc gcaggcactc ttctcccaca tttcgatgat
     7381 cgaagccagc cacggaatcc ggattaagaa cagggaggca attgcacgca tcattgcacc
     7441 cacaattaaa agcgacaccc aggcgctctc ggttggaatc tcgctgaaca actgggtcgc
     7501 agtagccggc gcatacggag aagtaacggt gtctgagagt gtgatccgga caatcctcga
     7561 ttcactgaag agtgcgtgaa ggtgcaatgc atccccttat cgggggatgg catttgtaga
     7621 ggaaacaacc tgtttcgcaa tgatgtgcca ggaaagagac aggcagcgcc gctttcggaa
     7681 aaactgccga cactgtgccc gggtagcggg accgggacgg gaccgcaccc ctgttatcga
     7741 attccggacg ggaaggatac ctatatgcgg aaaacgggtg aactcaatgg cagaggattc
     7801 acccgatgcc aggattgctg ctgctgacag gctattttgc ggtaatggta ttcattgtca
     7861 ccagcatgct ggcgatggga ctcaacatct ccatttgcga tctcatctcg cccctccgga
     7921 accggaggct cgtgatcctc tccctcctgg caaacttcgt gattgtcccc ctcttcgcat
     7981 tcgtgctcat ctatgtcttt ccggtgagca gggatctcgc ggcagggctc attctcgtca
     8041 gcattgccgc aggggcgccg tcaacgccga aggtagcaga actgaccgaa ggaaacatcg
     8101 cttatgcagt ctccctgacc ctcctgatga ccatcctcac catcatcctg atgccgatcc
     8161 tcgtccccta catcatggaa gggatgggga tgaacccggc aaaagtggca tttaacctcg
     8221 tcatcttcat gctccttccc attcttgtcg gcatgctgct ccgtaaccgg gcgccggaaa
     8281 gggcgcagaa ggtactcccg cttgccgaac tggtctcgaa tgcctccatc ggggtgatat
     8341 tcctggcatt tgggatcatc ttctttgcac acgtcgtgga tctcttcggg catcccgggg
     8401 gtttcgagat tgccgcgatc gccgtgctgt tcacggtcgg ggccctcctg atcgggtact
     8461 tcatgggagg ccctgagcgg ggccacaggg atgtccttgc gttcagcacg gggtttcgga
     8521 atatcacggc ggcactcgtt gtcatcacgg caagttacag cgaccttgac aacgacgtgt
     8581 tcctgatggt cctcatgatc acgctcttca gcgtgatcat cgtctcggcc attgtcggga
     8641 tcatcataaa gaaacggatt gatgccggga agcaatcgcg ccttcagcca tcctgaagtc
     8701 atccataccc ctcttccggc ctgaacgacc agggtctcta gaccagaatc tagaaatgac
     8761 aggcaaaacg atctcactgt atatggcacg atccctgacg atatacctcg catatttttt
     8821 ccaggccctc atcgccctca acgggatata tgcccttgtg acggggcaat accaggcgat
     8881 gttcaccgca ttcctcatgt tcgggctcac cctggtcccc tacatcgtgg cccagaggat
     8941 gcacatcaac ctcccgtggt ttgtgtacct gcttatcgcc ctggcactct ggatccacac
     9001 tgcaggatat atccaggggt actacaccct cttctacccg tattacgaca aggtggccca
     9061 cctgatctcg gggacggctg tggcgctcct cgggttcctc ggtgtcatct tcctcgacaa
     9121 gtactggaag atgaccctca ccaccccgtt catcatcgca tttaccatca tcttctccgt
     9181 ggcattggga gcattctggg agatgtacga gttcttcgtg gacatggtct ttggcgggtc
     9241 gcttgcagga ccgatgcaga acggcttaaa cgacacgatg ttcgacatga tcttcgtcct
     9301 tgcaggttcg atcatcgtgg ccattctcgg gcactcgtac ctcaagaaga acccaaagga
     9361 aaagatcacg ggggatgcct gaaccgcgtc cggttcctcc ctggcggttt gccccggata
     9421 cagggatatt tccaaggaca acgcatttcc gggaaaaatg ccctggtatc ctcttcccct
     9481 gggagaaaat cccccggacg atctctcact gcaccgggga tgccgggttg atgatcacga
     9541 tctcgggcgt cgcccctatg cggaggtccc agggaaggtt cgaagtccct atccccctcg
     9601 tgatgtaaac agggacgcga ctgtcccgga cgtatcccga gagttccatg atcccggcgt
     9661 cgttgatgaa ctgaagcgcg gtgccgccga actgcccgcc gtgggtgtgc ccggaaagga
     9721 tgagatccgc gtcccagtcg gccctgcact ccggttcgtg aatcatgtag attgaaaacg
     9781 catcggtggc gggaaccgcg ggggggtccg ccatccctgc ccacccgtca tcgactccga
     9841 cgagcaggag cggtgtcccc gatacctcgc gcatcacgta ctcgttcctc tgcaccatca
     9901 cgccatgtgc ttcgagggct tcctcgagcg ctgccgcgga tgcaaggttg gtggtctcgt
     9961 caaagaggcc gctcatgtcg taggtgagga ggtcccggtc cgtgcgcgag actgcgatcg
    10021 acttggcgat ggccgaggtg gcgtctatcc ctgcgtggta gtcatggttc ccgaggaccg
    10081 catagaccgg ggcatcgatc tcgctccaga tctcctggca ggagaggtta tcctcctccc
    10141 cgaagaggaa gtcgccccct atcaggacaa ttgaagggtt gagttcattg atcgtctgga
    10201 tgacttttcg cactttctca atattccctg gcttgacgtg cgggtcggcg atgaagacga
    10261 ttccctccgg tgcaccgtca atggagagaa ccgtgacctg cgggtgggcc ccctcccagt
    10321 atgcatagcc tgcgcctgcg ccggcaacta ccagcaggac ggcggagatc gcaaaatacc
    10381 gcgtggggag taagaacgac cagcgggaca tctgatggga tcaggtgggt caatcagcct
    10441 caaataggtg ccacatacgg caaaggggcc gatctcccgt ggcgggaccc cgaagaccgg
    10501 gatcacagag caggtgatgt attgtcaatc acccgtaccg tgaggttgaa ccgcttcgca
    10561 ccgccgatat cgcacccgtc gagtgagaaa atcccgtcaa aggtcgtggt ccccggatct
    10621 tttgccagga aacgccaccg gtaatatcca ttcgacttga aatataccat ggccccgctg
    10681 ctgttgagat cgagatcatc gggtaatacc gagaacgctc cggcattcag caattcaagg
    10741 ccggacccct cggctggcat caaccaccgg gtcgcaaggc tcgggatgat ccgaagcgta
    10801 atctcgagga tttcgcccct ctcaagcgta acgactctcc ccgaatcggt ctcgttcaat
    10861 gaatacctca ctcttcctgg aagtggctcg ctgatgggga ctgatgggtc cgtccttggc
    10921 ggctcgacgt agttcacgta tttcgggatg cagcgggggc tggatccttc agggggaacg
    10981 aattcgaaat acgaatcagg aattcccgag ttgactgcaa tcgactcgta gcggacctcc
    11041 cggttcggca gttcgctgcg gtcgatggtg cctggcggcg ccatgtctat gggcggaggt
    11101 gtcggggtcg gcaataccat gccgggtgca agggtcggtt gtcctgccac gctgctgtcg
    11161 tagtaagtgg caacctccca tgcaagaccg gttgaaggct ctacccgggc ccggacccgc
    11221 gagctgatgt agggagtgta ccggtcggac catgggtcgg tcacgacctc gataaggtac
    11281 cgggaagccc cgtgttccgt gtctcgctca agtatcgcaa aatcacggtc cgcgatgata
    11341 tgccggacca tccgctgata gtcatactcg cgggagaggt tcatcccgga atagagatca
    11401 tagacacggg ttcgggagtc gtaccacgaa gtggatgtgc cgctggaggt cgcaaacgat
    11461 ccctgctctc cagcagtttc aggctccatc tcaatccgcg taaaggaagg cgtcttgaag
    11521 tagaacgaga ttttctccac tcgcagatcc cctgcggcca ttcccgaata cacaacgtat
    11581 tccgactgca aatccctgat tgttgcggcc tgggcaaggt agcgatcggc aagctcgcct
    11641 tcagtcaggt tgcaggagtc agggatctcc gtgacacacc cggaaaagac cgcgaagaga
    11701 aggagcatcg cggcaaggac aggatatatc atccgggctg tcttctgcat ggtatccccg
    11761 ttcctcaaaa cggcacgata agtgtttcgc tttatggttt cagatcccct ccttctcccc
    11821 atcccatggt tatttaccct tgcacggcct atgataatat tccagacaaa ccctggattc
    11881 cgggagcgcc ttttgattgg acacgggaat ttcatatccg ccccgtatcg tttgccccgg
    11941 gcataccgca tgtatcggct gtcagaacga aagatgaatg ccgtggatca tcaatcctgt
    12001 tgctccacgc atcacacaga caggattaga tgataaagaa cagcgcaaga cccacggtaa
    12061 tcgggaccag gtcagggtat accatacgct ttacctgtcc caattgtgca aaagagaact
    12121 caatcgtata caatatgccc aaggccttct acaaggaatc aagggacgca gtgtgcggga
    12181 agtgcaggaa gcacttcacc gtgcttaccc ctgattgata tacagaaacg gcggggagaa
    12241 gaggagagat tcttcctttt ccctcccttt tctccctcct caaaaacccc tttcctgcgt
    12301 gattctgaac gaactcctca tccctgtgtg acattcgtcg tccagtactc tacaagcagg
    12361 atttccatgt ccgtggtaca cgtatcgcag gcaatcttcg ggttctgcac tgccaggcgg
    12421 aacatcactg cgtcaccggg atcaggggtg agaggagcat ccacgttttt ctggatgata
    12481 tacctgtagg gatcgaggct tccgaagtaa tactccttgt agcggtggcg atcctcatca
    12541 gtcgcatcat gggaccagtc tgccgattct gcaaccgtga cgtcaaccgg tatccagcca
    12601 tacccctcca ggtaatattc tgcccagaag tgttcacccg caagccccgg gaccagctgg
    12661 aaccctcccg tggaccgtgc ggggataccg agtgatcggc acagggcagc aaaatacatg
    12721 ctctgcgatc cgcagtcgcc gaacccagtc tcaaggacat aggtggattc ggggatccct
    12781 gcagtctcca gcatgaagtg cggaacgtgg ctgtagggaa gtgtgttcat gatatgccag
    12841 tagatcttct ccgcctggag gtgtgggttc gtctcgtcac cgacaatctc gtgtgctttc
    12901 ttctttatct cctgtgtgac ggcaatgttc ttttcagaca tcgtatactg caggtattcg
    12961 ggattgctca ggttgtaggc cttcaccctg gaagggtcaa tgacaaaccg tacctcgtgc
    13021 tgggtaaatc ggaacctggt cgtaatgttg aggagatcgc cctttacatc cgcgagcggg
    13081 atctccaggt acgcaagccc gatgtcggct gaggtatccg gccaggaacg tacgtactgt
    13141 gccggctcga tcgagaggat cccaacatcc gattgtgaac cggactctat gggggccggg
    13201 acccatatcc gcatggtgcc ggtctccggg agaagtactg ttggaagcaa gaggcgctgg
    13261 gttatttcaa atacgaccgg ttcgccgtag ggccccttgt tgggaagcgg agtggcgaac
    13321 gcaaggctgc acgtctcgtc atagaaaggt gaatggttga actcttttgt cattctccgg
    13381 gccagtgtga gattatgaaa catgatattg ctgactgtat cggagaaata gaggtgttcg
    13441 ccgtcactca ctatgcggat actcttctct tccgagagga gatcggcaat ctccctggct
    13501 gaaaattccg gatacgcctg gattatctct gactcgactg cggaccggtt gaacgggaac
    13561 tcgaagatca ttcgctgcga tacgagtgcc tccctcatgg ccttccgggc agagacagtg
    13621 tcacccctgg cctgataatc gctgtatgcc tgcatgtaga gttcagcggc agaacggtaa
    13681 ttcgatgcct cgtactccgc ggcggccttt gcggcgggtt cgtcaactgt ggaggaggcc
    13741 ccggcatcct tttccgatat gcagcctgcc gcaagcgtcg cggcagcgat aaggaacagg
    13801 actatgaggg aatatgataa gattcgattc atgcatcacc ttgcgttgag gatctattga
    13861 atagggaact gagcggaagt gactttaagc tgatgtgtga aaagttgcga aaattctcct
    13921 ttccggcctg aactgggtgg atcctgcatc aaggtgaatt ctggtcttct gtacaaatcc
    13981 aaacgcttat cgcccgaaac cgctcaacgc ctcttttatg aaggtgggaa ttatccgctg
    14041 cctgatgacc gaggacctct gcccggggac gatggacttt cgcgtgactg ccgagggtaa
    14101 gggtgcgttt gcagatactg gcccggtcga gatcgtcggc ttcgtgacgt gcggggggtg
    14161 cccgggcaaa cgggccatcc cgcgtgcaaa gatgctcgtg gagaggggtg cgcaggcgat
    14221 cgtgttcgcc tcgtgcatca ccagggggaa cccgatcggg tacccgtgcc cgcactatgc
    14281 cgacatgagg gactgcgtga agaaagccat cggcccggat atctccatca tcgaatggac
    14341 tcactgacgc agctttcccg cttcagcccc agatagggtc gtctccgaac cgctccatcc
    14401 agtagtcagc ggcggcatct gcaagttccc gtgcgaggtc ttcagccctg aaagagaacg
    14461 ggtggttcat gcagtactct acgaggagtt catacgcctc cctgagccgg atggtcatct
    14521 cgtgcgactc gacagggtct ttccgggaga catccgggtg ccactccctg acccgatcgt
    14581 ggtagagcgc acggatctcg tgaaggctcg cctgatccct gatcccgagg aggtcagccg
    14641 cgtccctcac caattcggcc gcaattttgg ccattgcata ctggtttcgg gagcgccggg
    14701 ataatggttg ccaccgctgc tgcagaaccg atcacttttt cgtcaccctc gcgagaactt
    14761 cgagtgagaa cgagtgccat ggcagaagac cggcccctca tctacctgaa taacgccgcc
    14821 accagctggc caaaacctcc cgaagtcttc gcagcggtct tagagtccct ctcgctcccg
    14881 gtctttgggt cggggcggac cacggtgtcc cagggcgagg attatgtcac gaaggcccgc
    14941 gacgcacttt cgggcctctt tggagtggaa accgccgagc acgtggtctt cacccagaac
    15001 gcgaccgact ccctgaacgt cctcatccag ggattcctcg aacgccacgc gggtgactgc
    15061 cacgtcctta ccacagcgct cgaccataac tctgtcctcc gccctctcca cgagtacgag
    15121 cgccagggga ggatccgcct gactgtcctc cccttcgaag acggccgtgt cagccccgct
    15181 gcggtcacag aggcaatcac ccccatgacc cggctgatgg tcacgacgca cgggagcaac
    15241 gtgctcggga ccgcggcgga catccgggga atcggagagg tgttacacga tcacggagcc
    15301 tatttcgtag tcgacggggc ccaggcagcc gggcatgtgc ccatcgactt tgagagcctc
    15361 cctgtcgatg cctatgcctt caccggccac aaggcactcc tcggcatcgc agggaccggg
    15421 ggctttgtga taaaagaccc tgaggcaatc gcaccggtca ggttcggggg aaccgggacc
    15481 gattccttct cgctccacca ccctgccggg atgccggagc ggttcgaggc ggggactcac
    15541 aactacccgg gccttgccgg gcttgccgcg ggcgtctcgt tcatcctctc gaccgggatt
    15601 gaaacaatca tggagaaggc agaacgccag accgcgtatt tagtcgggga acttgcaaag
    15661 gagcacaaca tcaggatcta ccaggaccgc ccggaccttc ccatcatcgc cttcaacata
    15721 aggaatcttc caaacgacga catagggttc atcctggccc gcaggtatgg gattatcgtc
    15781 agaacggggc ttcattgcgc cccgctcgtt caccgggcaa tcgacggggg agaggggtgc
    15841 gtacggctga gccactcctg gtttacgacc gacgaagaat gcaggatcgc ggcgggggcc
    15901 gtgagggaga ttgcgcagaa tgcggatcgt ccggtcagtt cggcataagg tctgcgcgga
    15961 cggctccttc atgaaggagt tcctcgtcga cgagcccatc cctgcgtcgt tcttttcgtt
    16021 tcttacaaac ttcgggaagg tcgaggcgct cccgcagatc ggcgaaggat tcttccggtt
    16081 cgagaagccg gactggttct ccatcaaggg gtttgccggc gacacgactg tcgaggtgag
    16141 gttcaaaaaa gaggtgatgg acctgacgat ggactttgcg tattccctct tctcctcgtt
    16201 ccagaatgag aaacccgacc tttcaggcct gaaacagcgg gaggaggcag tcgccgggcg
    16261 ggtccgccga cgcctccacg gggagtgagc gggggaagat tcgtgcccga agatgtcttc
    16321 gagcggtatg cggaggacta tgacctctgg ttcgaggagc accccgccga gtaccgttca
    16381 gaactcggcc gggttcgcgc actttcccct cccatagccc ccttttccct cgaagtggga
    16441 gcgggatcgg gacggtttgc cgcaccgctc gggatcgggc tcggggtcga gccctcggtg
    16501 gcgctctgcc ggatggcgcg atggaggggg atcgaggttg tccgggcaat cggcgaggcg
    16561 atccccttca gggatgcatc catcccgtca gtcctgatgg tgaccgtcat ctgcttcctg
    16621 gaagacccct cccgggtgct cgccgaactg cagcgaatcc tcgtccccgg gggcgctgtc
    16681 gtgattgcgt tcatcgaacg cggcggccca atacaccaaa aatacctcta tgagggaggg
    16741 aagggacgat ttctctcctg tgcacgcttc tataccaagg aggaggtgtt atctctcctc
    16801 gaaaataacg ggttttgggt caccgctgtg gatccccggg cagggttctg tgtcatcgcg
    16861 gcgcagaagg gctaatcgga gagggattgg gtttttgccc agattgtcat tggttccgga
    16921 atcaatcgac gacatttgat tttaatccca caataccccc tttttttgcc ggccgatttt
    16981 tccgaggcat ttatccgcat ttgcgacgtt gatagtagta ttgctgccgg taactggtga
    17041 tccctggtga agagcgtaac gatgcgtgaa ggagaagtcc tggtcctctc ccttgaggga
    17101 aggctcgatg cgcagggggc cgtggagata gacgcggtgc tgaaagatct cctccgtgag
    17161 accgacagca cgataatctt tgacatgtcg ggagtcacct acatgagcag cgccggcatc
    17221 aggacaattg ttgcaaccga gaagaggatg aaggagaggg gcggtcgaat ccacctctca
    17281 tgtctccagc cctatccccg ctcggtcctc gatatgacgg gtttctctac cgtgctctcc
    17341 atccattcca cccgcaaaga ggcggtccgg gcggcaaggg caacggtcgg acgggaaatt
    17401 ggagttcccg gacacgcccc tctcgccgtg cagaccaggg gcgcggagtt tgttgccacc
    17461 tgcacggggc aggatgcctg cacccttgat attaccggat ttcccaccga tgagaggagt
    17521 ggaggaaggg acactggccc cgccatccca gtcacgatcc ccgtgtccgc atgttccctc
    17581 ggattcggtt caccaggcct cccggatgac gatgaggaga gcgcgatggg ggactttttg
    17641 tctgtcgggc acatcgcggc gtgggtcctc cctgacagcg gcgacaccct cgactacctg
    17701 gtcctcgaga agaatgtggc aagaatcccg ctcgccacct cgtttctcat ctctccttcc
    17761 gcgccacccg ggtgcgaggt ccgggtgcat gccggatcac cgggcggaat cacgctctcg
    17821 gatctcttcg attccctcca tgagattgca aaagagatgg atccccgcta ctgtggtgtt
    17881 ctctgcacct cgttctgcgc cgaatctccg gacgtgcaga ccctcaatcc ggcggaaggc
    17941 acgcctccaa ctgccacgct cgcgggatgt gcggttattg tcgatgctgc agccctcccg
    18001 gatcacctgg ggggagtggt caccgatgcg ctggtccgcc accttcccgg aagaccgggc
    18061 atcgtcccac gggccacagc gctcgtcttc cgggatctcg ctgcaggcga gggtgagtcc
    18121 gcctgcgagg cggtcgcacg cgggctttcc tcggatgtgc aggcgctcct ccgtcatctc
    18181 tcgccccgca cgaggatatt tcaggcgact ctccagctct tcgtcatctc cgacatccgg
    18241 cttcataccg gcaccacgat cgtctttaag ggggatgtcc cgggatggaa ccccgactac
    18301 gagcggatca cgaagagtgt tcaccatgac tgctcggagg tgcgcctcca cccgatatcc
    18361 ggcggctatt ccgggtcgct cgtcttccgc gacgatgcct acgaccgcag cggcaggaga
    18421 gagatgcctt ttgtattaaa actcgaccgg tgggagaaca tccgggcgga gatcgagggg
    18481 tacgaggggc atgtgaaacg gtatatccag aataacgcga cccagatcat ccagaaggcg
    18541 cgaagcggcg agtacggcgg catcctctac acgttcgtcg ggatccaggg gccccagagc
    18601 cgcatcttct cgctcgagga ctattaccgc acccacccga ctgacgaagt gctcgcggta
    18661 tttgagatac tcttccggag agtgctcagg gcatggtacg gccagccgcg gctccgggac
    18721 cttcccctgt acgatgtcta cagcgacatc ttccggtacg aggatgtccg caggtgggca
    18781 gaatcccgct acagcatcac cgcggcggac gagacaatcg atctcccctt tggactgggg
    18841 aggtcggcaa accccctgca cttcatggaa cacattctcc cggtccgccg atcgcggact
    18901 tggagcgtgt acgaggggtc ggtccacggt gacctgaaca tgaagaacgt gctgatggac
    18961 gacgagcaga acctctggtt gatcgacttt gcgatgaccg ggcactcgca catcctgcgt
    19021 gacgtcgcaa aacttgaatc cgtgctgaag ctggagatga ttcccatcga gtcgcaggaa
    19081 cgcctctttg agcttgtcgc acttgagcag gtcttcctga ccccaaaaaa gctcggcgag
    19141 atcccttcgc ttcccgaagg tatcgctgac ccggatatcg caaaggcatt ccaggtagtc
    19201 catcagctca ggcggtacgc cgacaccatc acgctgctcg acgaggacat cctgcagtat
    19261 tacctggcgc tcctctatta cacgctctgt gtcccagcat tcgtgagcgt aaatgattac
    19321 atgcgggagt atgcctggat ctcctcgtcc ctcctctgcg aggccctccg gatacatggg
    19381 gagcactgat ctgcgacgaa tcgctatggc agggtttttg agggattgct gtccagaggc
    19441 atcggataag agttcccttt tcgcagggct gctgatgcgt gagacatcac tcatccagac
    19501 gaaccttcca gagcggtgct gccacctcca tcaccggtat cttgtgtgtt ctctcctcct
    19561 gcaatacgaa tacaggtact cgtaaaagtg caccggacgc gggtgtggac tggcataaac
    19621 gcagtcgggc atgcccattt gttgtattgt acaaatacaa gcaaacacgt cggatttgta
    19681 acattacgca gcgattgttt gcggaaatga atttaagccc tcatccccat ggatcctctg
    19741 aggtgtaaca gatggatata gtaaacgcat tcgttggact gattcagctc gtcatcgcca
    19801 taatcctggc agtagcagcc ctctacatcg ggtacgccac gttctcgaag atcaccaagg
    19861 gaatggacga ggccgctgaa ctcagaaaag gcaatgtcgc ggtgggcatc gtcatcgcct
    19921 ccatcctctt tgccatcgct atcgtggtac agtccgggat cgccgggatc tccataggta
    19981 tcatgaacgc tcttgcaggg gactggttct cgctcatcgc cgcagtcatc cagctgatcc
    20041 tcggcatcat ccttgccgtc gcggtcagct actttgcgct gcacgtcatg gaccggctca
    20101 cgaagggact caacgagttc gaggaattga agaaaggaaa tgtcgcggtt gccctcctga
    20161 tggcaggagt catcattgcg accgcagtca tcatccagtc cggggtcatc ggcatcacct
    20221 cggcactcat ataacccaac aatattttta ttgacctgta gcgtcaggcg ccagggttgc
    20281 cctgcgacag gttgcggaaa gcgaattccc cgccttccgg tcgttcatgg ctttccccgg
    20341 cgactctatt ctccttcagc caccgctatt caggcatgat gaatggattc ccattatcgt
    20401 atccgataag agactgtgcg tatactcccg gttatcggga gaggatgaat gctgcaatgg
    20461 cagagggaaa aaaaggggaa ttacttgatg atcttcttca gcatgttgtc tatcatctca
    20521 ggggcacggt cctttaagcc aaatgccagg aacaccgcga cgccgacacc aactccaatc
    20581 cccacacccc atgcaatcgg cgtgatgaac gtgtagatga ttgtaaggtc gatcagcagc
    20641 tgggagagcg cgagaatcag gaccacgaag tagaagaata cccgcagcag catgatgatg
    20701 acaccaagga actcgatatt ctgggtcttt ccccatgcat gcatcaggtc cgagaagtag
    20761 tcgacgatga cgaaccctgc aaccaggatg ataaggaaca tcgcgacgtg gggcatgtag
    20821 aggatgatct tctcgaagag ttcgctgatg aatccgatct gaaggacgct tgccgcggcg
    20881 gcgattgcaa tcaggtagat gaaccatctg acaatgagat caaagagccg tacaatatgc
    20941 attcccgtcc cttcaatcgc ctttccaagg ctggtcttcc ggagtgcgtc gtcaactccc
    21001 accttgtcga ggatcttcga tacgagagaa ccgagaaccc ggccgacaac ccacccgatg
    21061 agcaggatga tgattgctgc aatgaggtac gggatgtatg cgacaaccga ggagaacatc
    21121 tgggtcaggg aatcaccgag ggaggtcatg gtctgtgtag ctatgtctgt tgttgccata
    21181 acgatcaaaa ccccaataag ggacacgagt atttaacaat attccacgtg gcatacgttt
    21241 ccaattaata ttttgaaaac tccagcccag attccggttt cacgcgacgg aataggtgaa
    21301 cggcggaatc aactgaatgg caggcgttcc gcacaacaat aggtaaaatc ccgggatcat
    21361 ggagcgtgtc gcaaagggag atgccttttc attgagaaag gtcaggggtc acatcatccg
    21421 tgttcggatg ggacacgtgt aatgatgacg atcatgtgaa gggtagagtg tcctctttcg
    21481 tcaattgcca ggaatatacc gggtagcatc tcgaaaaatg aggaaattaa tatggttctg
    21541 attatttccc gaggaatttt ttgaagattc ccgagacgcc ggctgcctct ttctgcgatc
    21601 ccagcatctg ctctgccatg cgtgcggcat ccggtgatga ctgcatcgcc atcttcgact
    21661 gctcgccgag gaggctggtg aggccctgct ggtccatctg tttgccaccc atgctcttcg
    21721 tgacgtaccc catgaccatc ggggtcgcga tggcgaggat cttttcaacc actgcagagg
    21781 gaagaccggt cttctgtgca atcgcattcg agatcggcgc catctggctt ccgagaaggc
    21841 tgcttgccat cccggatccc ccggatgcgg ctgaactgcc aaggaagcca cccaggttat
    21901 caagggggtt actccctccc atctgcccca tcattgacgt gatcatgtct gctccacccg
    21961 gtttctgtga ggtctgtgcc atggagccca tgatcatcgg aaggcccata ccaagtgcgg
    22021 actgcacact cttctcatcc gaaccgacag atttgctgat catggagaga ttgtctcc
//
LOCUS       DAOQYR010000028        21592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_2795_length_21592_cov_8.175744, whole genome shotgun sequence.
ACCESSION   DAOQYR010000028 DAOQYR010000000
VERSION     DAOQYR010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 21592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21592
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2795_length_21592_cov_8.175744"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PKM87_05035"
     CDS             complement(<1..440)
                     /locus_tag="PKM87_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HNQ29467.1"
                     /translation="MDYGNMLGDSLGYAKDGLVGHWKRWILLIISTIIFPLIMGYTME
                     IWRGKTPAPEPAQWGKMFIDGLKLLVAAIIYAIPVILIILVFGGFAFFTAIQEAAMSG
                     DPEFFTNNMEVLMPLVTAFMVGLLVAFIVAIILSLFSTIGFVRMA"
     gene            complement(663..1361)
                     /locus_tag="PKM87_05040"
     CDS             complement(663..1361)
                     /locus_tag="PKM87_05040"
                     /inference="COORDINATES: protein motif:HMM:NF024595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HNQ29468.1"
                     /translation="MAYTETDIPASLIATLIMGFVIAFVMALILGAFSMGYLLKILRG
                     EVPLPEVTGFGTMFVDGIKLLVIEIIYLIPVLIILAVTAGAALISALPILMSMPAEPD
                     FEALMPGLMPIIGGMIAGILIAVIAAIILWLLAVVGVVRFARTGKIGEAFNFSAILAT
                     IGKIRWGTYILALIIVVAIVAIVEVILSFIPYIGSLILILISPFIMVFMHRYVCILYD
                     SAGNPADPSRSFTP"
     gene            complement(1597..3081)
                     /gene="purH"
                     /locus_tag="PKM87_05045"
     CDS             complement(1597..3081)
                     /gene="purH"
                     /locus_tag="PKM87_05045"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNQ29469.1"
                     /translation="MKWALLSVWNKEGIVDLARELHRHRLQLLSSGGTGAALKEASIP
                     FTEVSAYTGSSEMMDGRVKTLHPRIHGGLLGRRGIDDGVMREEGIHQIDLLVANLYPF
                     EEMANKGLPLGELVEYIDIGGPAMIRAAAKNHKDVAVVTDPSDYPLVKEALESGGFTA
                     DQRLLFAKRAFARTAAYDAAISNYLHLLGSEFPGVYTVQFRNGRTLRYGENPHQRGAV
                     YGQAGVAGSEPLQGKQMSYNNYLDLSSAAGLVREFSEPAAVIVKHNNPCGVAVGKNLA
                     DAYIQARDVDPVSAYGSVVALNREVDRHLAEDIASTFVEVVAAPEYTRNALQVFGKKE
                     NLRVLHLPAAERGWELRSIDGGLLVQDTPPYQERWEVVTDRDATPAEMKAMKFACIVC
                     AHTRSNAIVFADATRTLGIGAGQMSRVDAAKIAIEKSRFSLAGSAVASDAFLPFPDTL
                     EVAAAAGATALVQPGGSIRDKEVIEAANRLDVAMVFTGVRHFRH"
     gene            3210..4553
                     /locus_tag="PKM87_05050"
     CDS             3210..4553
                     /locus_tag="PKM87_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HNQ29470.1"
                     /translation="MREPPVKKVLYWCPRCNIPLLGRSCMCGAEGRAIPLLRPYDARP
                     ALKADRDLLAWLLDERYGTDTLPRVIVLNKTGGIDRKDLVIANGGRFGWLSYDPAQQR
                     FDLELTFESLPFILPSARRNVLELAQLQGAARDVESGRRIGGKRYPAEGKVPDGGVLV
                     RWNGHGGVGVRTGGMVKVKEVGRVSPGEYPDPGWDEVISRNRLHLKNLERNAIRFIRQ
                     QAEGHECVNISCSGGKDSTAALELARRAGFEDSYFVDTGMEFPETLGFVKSLGIRTIL
                     RARNFWREVERRGPPRKDDRWCCELQKLAPVKEWLEGKGECITIQGNRWYESFARAGL
                     PAAARNPFHPMQINVSPIRNWRALEVFLYIWWRNLPQNPLYEMGMERVGCWMCPAMLE
                     SEFAVVRTLHPDLHRVWMEFLGEWFRDRGLDKEALSAGAWRWKQLPPKMRGWDRD"
     gene            4695..5222
                     /locus_tag="PKM87_05055"
     CDS             4695..5222
                     /locus_tag="PKM87_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNQ29471.1"
                     /translation="MAESREEENFDSDAQKEQESGTNSVEPPSGREELQKKYDELNNR
                     FIRLAADFDNYRKRSDRDVETRVKFAIERFAVDLLEVVDNFDRALSADPHSAREGLEQ
                     IHKQFQAILERHGICPIEASGKKFNPEEHEAVYCEPSGEEDGMVLKEICCGFRMHDKV
                     IRYAKVVVSKGKETE"
     gene            5225..7069
                     /gene="dnaK"
                     /locus_tag="PKM87_05060"
     CDS             5225..7069
                     /gene="dnaK"
                     /locus_tag="PKM87_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNQ29472.1"
                     /translation="MVKEKILGIDLGTTFSCMAIMEAGKPIVIPNAEGQRTTPSVVGF
                     TKDGERLVGSLAKRQAVTNPTRTIQSIKRKMGTTEKVKIDDKSYTPQEISAMILQKMK
                     TDAEAYLGEKIRKAVITVPAYFNDAQRQATKDAGQIAGLEVLRIINEPTASALAYGID
                     KEQDVTVLVYDLGGGTFDVSILTLGDGVFEVKSTSGNNHLGGDDFDNRIMDYLIEEFQ
                     KKEGINLRTDPIAIQRLRDAAENAKVELSQKVKTNINLPYITTGPSGPKFLDIDLTRA
                     KLEQLIGDLVESTVGPVKQALSDAKLTPDQIDHVLLVGGSTRVPLVQETVRKILGKEP
                     DKGINPDECVGLGAAIQGAVLSGETKDIVLLDVTPLTLGIETLGGIATKLIERNTTIP
                     TRKGQIFSTAADGQTSVEIHVVQGERALAKDNFTLGRFQLTGIPPAPRGIPQIEVTFD
                     IDANGIIHVSAKDLGTGNEQAITIKGDRKLTEDDIKRMTEDAKKFEAEDAKKKEEIEL
                     RNNADIAIFSAEKMLKEQGNNIEPADREKVETGVADLRKVLEADNSDEIRAKMDTLTE
                     AVYTITTKIYQKAQAERQAQQAESGDKGGSTTPGDENVVDADFKVKDE"
     gene            7075..8205
                     /gene="dnaJ"
                     /locus_tag="PKM87_05065"
     CDS             7075..8205
                     /gene="dnaJ"
                     /locus_tag="PKM87_05065"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNQ29473.1"
                     /translation="MSEGDYYEILGVSRNAEEKEIKKAYRNLARKYHPDVCKEPGAEE
                     QFKKINEAYSVLSDSQKRAQYDHLGHDTYTSASKGSYSGTGGFHGGGFSADFSGFGDI
                     FDFFGGGFSGSRQTGPQSGSDLLMKMQISLEDAVFGADREIEVNHTEACTHCNGTGSE
                     SKKLVKCPRCGGSGQMRQMSQTIFGQFVRMSTCTECRGRGRIPEKRCTVCKGSGHTQV
                     HRKVTVHIPAGIDSGMRLRMEGYGEAGDYGAPNGDLFIEVYVRPHDRFRRTGDHLETE
                     VEVSPAQAVVGSIVDVETIEKRTVELTIPAGIQHNTALKIPGEGVRRRGKPGDLLVRV
                     RVVIPKQPKAEIRSLYEKILEMEGHRMADSRKGFFSGFMGKK"
     gene            8338..9300
                     /locus_tag="PKM87_05070"
     CDS             8338..9300
                     /locus_tag="PKM87_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNQ29474.1"
                     /translation="MHLLVELAGDHPQLPFAELDILGPVLERGTQVALVDCQHAEEIP
                     RLALAHSVMEHLGSCPAEKGAFSRMLNDLAISSPRPFAGRVKKVYGNTMDASQAELER
                     LIGSLVQGKVDLQNPAEEFRAIVAGSRCYLGRVLWRSDPRRFGDRRPGDRPFFHPGVM
                     MPRMVRALMNISCARQGERLLDPFSGTGGMIMEAALLGIIGIGSDIDPEMVRGSRMNA
                     REGVILRADGRRLPFRDESFDAVVTDLPYGQSVSIRARSLDSLYHDALGEISRVVKPG
                     RRVVLVTHRDIRMRAGNFMRVEGCYSQRVHRSLTRNILVLVRDG"
     gene            9387..10190
                     /locus_tag="PKM87_05075"
     CDS             9387..10190
                     /locus_tag="PKM87_05075"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNQ29475.1"
                     /translation="MKEGEVRGGGVIWTCSRTDPGVRDRNEDASGTFTLPHREGALYL
                     LAVADGLGGHPAGEVASAIAISALRSAVSSTVEALEGMDVSSLQAILATGFSHANREV
                     IRYAATTPCCLGMGTTLVAALMNGEGAGVVGNVGDSRAYLFGEGTVRITRDHSRVQEM
                     VDQGLISQEQAGNHPLRHIVTRIIGRTDDIPDFFPFRLKDDLLVLCSDGLVDGVSEGE
                     LQSLAGRHGSPDICERLVEYARGRSRDNITIVAAGRRRGLAFHGDCILA"
     gene            complement(10207..10923)
                     /locus_tag="PKM87_05080"
     CDS             complement(10207..10923)
                     /locus_tag="PKM87_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HNQ29476.1"
                     /translation="MDETIMKRMDYKDASDALKKALGLNYSPVAIRMATSHDDIPDGM
                     KQLEGNFRHCKMVDMARREGKVFYAKVENHECMGGAWALGLRPLTETLKNGHFYYKLG
                     KFESTAACKRTIDRIPHVESGVTVATLYAPLEKTNFSPQVVLIIAPARVMLKLAQATL
                     FSLGGRISSEFSGIQSVCADACAQTYLSGKANYSLGCDGSRKFSGIEDGEMVMGFPAE
                     MLPAMVEAVQIVTAAPGSKK"
     gene            complement(11006..11818)
                     /locus_tag="PKM87_05085"
     CDS             complement(11006..11818)
                     /locus_tag="PKM87_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNQ29477.1"
                     /translation="MKIVMVSRIDDEKALEYTRDLGDALLQDENQVCFEPATARALGV
                     DAAWTWDSQSDLVVVVGGDGTILHSVQKMEKQVPVLGINWGEVGFLADLEPGEARAFI
                     KSLRPGFAIEPRMRISLAREGVVLGQALNEALIVTNRPAKMLRFVVVVDGIPAERFRA
                     DGLLISTPTGSTAYAMSAGGPIVDPRIEGFLLVPLAPYMLSSRPHLISNERSVEVRLE
                     SAKPASLVIDGQKSIELGMEGSIVVTRAARPALFVDAKKNFFRKVDHKLRRL"
     gene            complement(11829..12605)
                     /locus_tag="PKM87_05090"
     CDS             complement(11829..12605)
                     /locus_tag="PKM87_05090"
                     /EC_number="3.1.3.11"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     fructose-bisphosphatase/inositol-phosphate phosphatase"
                     /protein_id="HNQ29478.1"
                     /translation="MEFLDACTLLAREVRGAIRDLVGSDEGGRTVRMGADRTPTKLID
                     QVAEDCVLRFLQDHPLCRTLISEEAGKVEISGDAGTIFLDPIDGTYNAVAGIPFYALS
                     LAYAEGGCVLQGFVEDLASGETFTAIRGKGAWLDGRPMTASRTTNLDESAMSIYGRKF
                     DPRRVMHIGQKIRRWRLLGASALELSYIGCGRIDGFIDIRGTLRVTDAAAGMLICTEA
                     GGKVSDLEGMPIHFPDEVTIGRCMVATNGVLHHKVIEYLR"
     gene            12694..13077
                     /locus_tag="PKM87_05095"
     CDS             12694..13077
                     /locus_tag="PKM87_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-5A"
                     /protein_id="HNQ29479.1"
                     /translation="MKEQTEVGKLKEGRYMVIEDEPCKILGISVSKPGKHGAAKARID
                     AVGLFDGVKRSIVQPVSAKTYVPVVERKSGQVITISGNTVTLMDMKEYHNFEIEVPAD
                     KMDRIEMGKEIQYMEALGKRKLDLD"
     gene            complement(13249..13992)
                     /locus_tag="PKM87_05100"
     CDS             complement(13249..13992)
                     /locus_tag="PKM87_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29480.1"
                     /translation="MEKRDMAILLVVLGVVVVMAVVVKPILTGKSPDLTLPFLPGSSP
                     GPTSPPPGYQEVATPSTPEKPASKAPTPTPTWSGTSQSLGYVSLQSPASTPTFTRLPE
                     ETPVPERLLTYASIESKGGGTTQTISMPFPYWELHYTVDPWKTTFFGETESKAAGIAS
                     FTASEVFPSFSIEVKDAADHSLVRRIEPRGGLDAELWEKEKENDPRPWVEKFYEGTTT
                     RSYYFVVHTHMVYSYRMEVKVPERYLGKY"
     gene            complement(14046..14498)
                     /locus_tag="PKM87_05105"
     CDS             complement(14046..14498)
                     /locus_tag="PKM87_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNQ29481.1"
                     /translation="MEKLVVQLDFLEFLPTHTCDGDDSSPRIRLGNLASASLAIMAVN
                     PFIPSCCSFSPWLAWNIPPMEIIPPGLPKEGVVESPVRAFQGRNDYGRIGYTGPCPPR
                     GSTHRYTFKVWGLDTMLDLAPGANKADLVGAMRGHVLQYGETAALCTR"
     gene            14563..14982
                     /locus_tag="PKM87_05110"
     CDS             14563..14982
                     /locus_tag="PKM87_05110"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HNQ29482.1"
                     /translation="MMAMTLYGYTDGASRGNPGPAACAFLLRDQEGRLLAKEALVLGT
                     RTNNEAEYHAVIEALRKAGSFASRNVVIYSDSQLVVRQLRGEYQVRKPHLRELHGVAK
                     DLERSFLSVEYRSVGRDDPWITMADRLCNEALDSANA"
     gene            15075..15965
                     /locus_tag="PKM87_05115"
     CDS             15075..15965
                     /locus_tag="PKM87_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNQ29483.1"
                     /translation="MTNEAGNTPEECNGVCEGCPSAQQCDSDKKDPKAKGLPPKAEID
                     VDHVILVLSGKGGVGKSTVAVNLSFALSAQGYNVGVLDLDIHGPNIPKMLGLEGQHLA
                     AMGKKIQPLRVTGNLSVVSMAFLLPSISTPVIWRGPMKMSAISQFLNDVDWGHLDYLV
                     VDLPPGTGDEALSIVQLAPNVRGAVIVTTPQEVATMDAMKAAKFVEQLNIPVLGIVEN
                     MSGMICPHCKKEIDLFGKGGGEKIAAELGVAYLGSIPLDPEMRKAGDEGKPFIIRSKD
                     SPTWKSVDKVMEALVRVVEE"
     gene            15966..16274
                     /locus_tag="PKM87_05120"
     CDS             15966..16274
                     /locus_tag="PKM87_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29484.1"
                     /translation="MTLLEVLTGEEKPLDIYDDIVPVLEEINLFPARVEGIYRKAMTY
                     DDDSLDRLRFALLRLQIHADIHRNEDMERAQAIKYASVVLEKVIFGSLMMGHEELEHD
                     "
     gene            16393..17925
                     /locus_tag="PKM87_05125"
     CDS             16393..17925
                     /locus_tag="PKM87_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ29485.1"
                     /translation="MEKYAFVARVPDQPGSLHKAAEIISRHQGNINRIQFDRRIDPQT
                     VFFEVTSTPEARKEIREGLLDLGYLQTALKPLSFLKISIHIPHTPGALLEFLRCTTAL
                     KANIALIDFDDRGSNPDRLTVSLNLEDSSAVSSLVDQLKTRYRIEVLEYDTSGKHLDD
                     AVFYLRFAQAIRGIVGESEDAFLLRLLGDINHIVQELMNRGQDPRLVFESILMTGETL
                     RNTSGTGFYAEVQSVRLADDLTLVCIQPPCGGNVFLLDTPAECVMVDTAYGIYSHDIA
                     TVIRTTQPGALDRLSRIVITHADADHCGAGGHYPVPALIHPGTMDIIRVSNRAYGSRS
                     QSSILEEVYTTLINLFSQFCPPRDAVLLPCQGEEKRGCFPVIAGLEVGKHRFDVLEGL
                     GGHLHGQVYLYSSTLGVFFSADTVINFDHLTPDRARYNTLAVNLVTSVNVDSGKAKEE
                     RSAILDIVRGTAGPFYGQRERCLVCGGHGPVSVLEGRILSPYGTMVHIPPAAEGCPRE
                     RP"
     gene            complement(17880..19169)
                     /locus_tag="PKM87_05130"
     CDS             complement(17880..19169)
                     /locus_tag="PKM87_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNQ29486.1"
                     /translation="MSPPAPCDFMQSLDEYLRDHIPELVALYRDLHAHPELSGQEART
                     STVIATEMGSAGFRVTRGIGGHGVVGVLENGIGPVVMIRADMDALPIDEDTGLSYASL
                     ERAVAPDGKEVGVMHACGHDLHCTVLAGTAHYLSGIRDHWTGTVVLVAQPSEEKGSGA
                     QGMIDDGLYTRFPRPDCALSFHVSPELPAGHVGYREGVFTAGSESLDIIVRGIGGHAA
                     HPDRCRDPIVLAAQLILQFQTIITREVPAGEFGLITVAAVHGGTKHNSIPDQVNLQVN
                     IRFFSRAVRDLILDSIARVCEHSARSMGMPEDRLPVITLIDESVSPIENDPELTSGII
                     SALAPCVGRDRIHRMAPLTGSEDFGLFGEVSPPVPLCYLRLGTGSLGEIYLHSPRFSL
                     DPEDCTRNGVVVMSLAALSVLGLQGRSRGHPSAAGGM"
     gene            complement(19166..19984)
                     /locus_tag="PKM87_05135"
     CDS             complement(19166..19984)
                     /locus_tag="PKM87_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNQ29487.1"
                     /translation="MDPIPYYQDGHFTLYHGDCLEILAEMPEGAFDLVFADPPYNLSN
                     GGFTCQSGRRAPVDKGAWDASNGIEEDFLFHRDWIKACRRVMCEDGSIWISGTYHSIY
                     ACGFALQREGFHVLNDICWFKPNAPPNLSCRYFTASHETLVWARKSRKGTHVFNYQDL
                     KCGDWSEDALKRRGRQMRTVWSIPTSRASEKGNGSHPTQKPLALLTRIVAACTTEGDR
                     VLDPFCGSSTTGLAAWSLGRQFVGIDTEEEYLEMSVRRYLSMKANGNAMRRKAK"
     gene            20181..20780
                     /gene="hxlB"
                     /locus_tag="PKM87_05140"
     CDS             20181..20780
                     /gene="hxlB"
                     /locus_tag="PKM87_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HNQ29488.1"
                     /translation="MAAHRVQDMMRLMARRIGRIAESLSDDEVEEFVEEILRARRIYV
                     MGAGRSGLVAKAFAMRLMHLGLSSFVVGETITPAMSVGDLIVVFSGSGRTKTVADIAE
                     TAKEIGGRLALITSNRDSRIGRIADVIVIIERHRDEVKDDGAEFEIRQMMGEHKSFAP
                     LGTLFETAAMIFADGVISRLMEITQIDEKALKDRHANIE"
     gene            20792..>21592
                     /locus_tag="PKM87_05145"
     CDS             20792..>21592
                     /locus_tag="PKM87_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNQ29489.1"
                     /translation="MEKMRCAGRCLGIEISGIRKLFEAAGPGSINMGLGQPDFDTPGH
                     IKEGAIRAIREGKTAYTSNMGIPELREALSSKFRNENGLSYSPDQILVTAGASEALHI
                     IMHALVEEGDRVLYADPGFVSYGALAVLAGGRPEGVPLDSNLRIDVEAAKKMMDGARL
                     FVLNSPGNPTGAVETKENIRALVEYGDDRGVTIVSDEVYEHFIYGATHWSAARFGEDV
                     ITVNAASKTYAMTGWRLGYLAGPKEYVEQCLKVHQYCQACATSISQYAA"
BASE COUNT         4832 a         5967 c         6491 g         4302 t
ORIGIN      
        1 gccatccgga caaacccgat ggtggagaag agcgagagga tgatggctac gatgaaggca
       61 accaggaggc cgaccatgaa tgccgtaacg agcggcataa gtacttccat gttgttggtg
      121 aagaactcag gatcaccgct cattgctgcc tcctggatgg ccgtgaagaa tgcgaaccct
      181 ccgaacacga ggatgatgag gatgacgggt atcgcataga tgatcgcggc gacaaggagc
      241 tttaagccgt cgatgaacat ctttccccac tgtgccggtt cgggagcagg cgtcttcccc
      301 cgccagatct ccatcgtgta tcccattatc agggggaata tgatcgtgga tatgatcagc
      361 agtatccagc gcttccagtg gccgacaagg ccgtccttgg cataaccaag agaatctccg
      421 agcatgttgc cataatccat gtgaatcacc acatattgca gagatatgtg ccatcagttg
      481 cgatttaaag gtatcctcga acttgagtca atcgactttt ttttacaggc ggatgttcat
      541 ttcgtgtccc gttggaacgt gaatgataaa atatggtctg actgtggtgt cacgacacct
      601 ccgttcagga catacaagga tacagtagtt ttttgtcagg caagtgggac gaaaaaaaag
      661 tgtcaggggg taaaagagcg ggaagggtca gccgggtttc ccgcagaatc gtagagaata
      721 cagacgtacc tgtgcatgaa aaccatgata aatggtgaaa tgaggatcag gatgagagaa
      781 ccgatgtagg gaatgaaact taagataacc tcgacgatag caacgatcgc caccacaatg
      841 atcagtgcca ggatgtaggt tccccacctg atcttcccga tggtggccag aattgcactg
      901 aagttgaagg cttctccgat ttttcctgtc cgggcaaacc ggacaacccc gacgactgca
      961 aggagccaga ggataatcgc tgcaataact gcaatcagga tgccggcgat cataccgccg
     1021 ataatgggca tcaggcctgg catcagtgct tcaaagtcag gttcggccgg cattgacatg
     1081 aggataggta acgcgctgat gagtgcggca cctgcagtga cagcgaggat gatcaacacg
     1141 ggtatgagat agattatctc tattaccagg agcttgatcc catcaacaaa catcgtgcca
     1201 aaaccggtca cttcgggtag cgggacttcg cccctgagga tcttgagcag gtatcccatg
     1261 gaaaaggcac caagtatgag ggccatcacg aatgcaatga caaaccccat gatcaacgtc
     1321 gcgatgagcg aggcaggaat gtccgtctca gtgtacgcca tcaatgaagc ccccatgagt
     1381 aacactaaga gaatgaatgg aatgactgga aggagggtca gcactatcag cattatccag
     1441 gtctttgggt tttttgtaag cccatccttt gcatactcaa acgaatcgcg aatcattttt
     1501 ccgtaatcca tatgaatcac cgatattctg gagagcaggg tgggatctcc gcttattaat
     1561 aggtatccga aaaaaagggt gagcaggcgg tccctttcag tgccggaaat gccggacacc
     1621 agtaaagacc atcgcgacat ccaggcggtt tgcggcctcg atgacctctt tgtcccggat
     1681 tgatccgccg ggctggacaa gagcagtggc cccggcagcg gcagcaacct ccagggtatc
     1741 agggaatggc aggaaagcgt ccgaagcgac cgcggatccc gcaagggaga accgggattt
     1801 ttcgatggcg atctttgccg cgtccacgcg gctcatctgc cctgccccga tcccgagcgt
     1861 cctcgttgca tccgcaaaga cgatggcatt gctccgggta tgagcgcata caatgcaggc
     1921 aaacttcatc gccttcatct ccgccggggt ggcgtcccgg tcggtgacga cctcccatcg
     1981 ctcctggtag ggaggggtgt cctgcacgag caggcctcca tcgatgctcc gcagttccca
     2041 gcctctttca gcggcaggga ggtgtaacac acggaggttc tccttctttc caaatacctg
     2101 cagggcgttg cgggtatact cgggggccgc cacgacctcg acaaaggtgc tcgcaatatc
     2161 ctctgcgagg tgacgatcca cctccctgtt cagagcgacg accgatccat atgccgagac
     2221 cgggtccaca tcgcgggcct ggatataggc atcggccagg ttctttccga ctgcaacgcc
     2281 gcatggattg ttgtgcttga caatcactgc tgcgggctcg gaaaactccc gcacaaggcc
     2341 tgcagcggac gagaggtcaa gatagttgtt gtaggacatc tgctttccct gcaggggctc
     2401 actccccgcg acaccggcct ggccgtatac ggcaccgcgc tggtgcgggt tttctccata
     2461 ccgaagcgtc ctcccgttcc tgaactggac ggtgtagacc ccggggaatt ctgacccgag
     2521 aaggtggagg taattgctga tcgccgcatc gtatgccgct gtccgggcaa atgcccgttt
     2581 tgcgaacagc aggcgctgat ctgccgtaaa ccctcctgat tcaagcgctt ctttaacaag
     2641 cgggtaatcc gagggatccg tgaccactgc cacgtcttta tggttcttgg cagcagctcg
     2701 gatcattgcg ggccctccga tatcgatata ctcgacgagc tccccaagcg ggaggccctt
     2761 attcgccatc tcttcgaagg ggtagaggtt cgcgacgaga aggtcgatct ggtggatccc
     2821 ctcctcccgc atcactccgt catcgatccc cctcctgcca agaaggccac cgtggatgcg
     2881 ggggtggagt gtcttgacgc ggccgtccat catctcggac gagccggtat acgccgatac
     2941 ttctgtgaag gggatggaag cctccttcaa tgctgcgccg gtccctcccg agctgagcag
     3001 ctgcaaccgg tggcggtgca gttctcgggc gaggtcgacg ataccctcct tgttccacac
     3061 agagagcagt gcccacttca tggtcatcta ttgggagata atgggataag agagtgatga
     3121 aagggatcat ggaaaaggag acggcggtcg tcacccccct ttcgtggggt caagcattat
     3181 cctgtcacgc catgatacct atctgcagga tgagagaacc ccccgtcaag aaggtgctgt
     3241 actggtgccc gcggtgcaat atcccgctcc ttggaaggag ctgcatgtgc ggtgcggaag
     3301 gccgcgctat cccgctcctt cgcccctatg acgcccgccc cgcgctcaaa gcggaccggg
     3361 acctcctggc ctggctgttg gacgagcggt atggaaccga cacactccca cgcgtcatcg
     3421 ttctcaacaa gacgggaggc attgacagga aggacctggt gatcgccaac ggaggacggt
     3481 tcgggtggct ctcgtacgac ccggcgcaac aaaggttcga tctcgagctc acgttcgaat
     3541 ccctgccgtt catcctgccg tctgcaagga gaaatgtcct tgagctcgcg caactgcagg
     3601 gtgccgccag ggatgttgag tcaggaaggc ggataggtgg gaagcgttat ccagcagagg
     3661 gaaaggttcc ggatgggggg gtgctcgtcc ggtggaatgg gcatggcgga gtgggagtgc
     3721 gcacaggggg aatggtgaaa gtaaaggagg tcggccgggt ctcacccggg gaatacccgg
     3781 atcccggctg ggacgaggta atctcccgga acaggctgca cctgaagaac ctcgagcgga
     3841 atgccattcg cttcatcagg cagcaggcag agggccatga atgcgtgaat atctcgtgct
     3901 cggggggaaa ggacagcacc gcggccctgg aactcgcccg gagggctggg ttcgaggaca
     3961 gctactttgt agacacaggc atggagtttc ccgagaccct cggtttcgta aaaagtctcg
     4021 gcatcaggac gatcctcagg gcgaggaact tctggcgcga ggtggaacgg cgtggaccac
     4081 cgagaaaaga cgaccgctgg tgctgcgaac tgcaaaaact tgctccggtc aaggaatggc
     4141 ttgaagggaa gggggaatgc atcacgatcc aggggaaccg ctggtacgaa tcatttgccc
     4201 gggcaggcct tcccgctgca gcgaggaatc cctttcaccc gatgcagatc aacgtatcgc
     4261 cgatcaggaa ctggagagcg ctcgaggtgt tcctctatat ctggtggcgg aacctccctc
     4321 agaatcccct ctacgagatg ggcatggagc gtgtcgggtg ctggatgtgc cctgccatgc
     4381 tcgagagcga gttcgcggtt gttcgcaccc tgcaccccga tctccaccgg gtctggatgg
     4441 aattcctcgg agaatggttc cgggatcggg gtctcgataa ggaggcgctt tctgccggtg
     4501 cgtggcggtg gaaacagctt ccaccgaaga tgcgcgggtg ggacagggac tgaatatctt
     4561 attccgacct gcttgcagaa caaaccgaat ttcaaaccgt ttttgattga atttcacctg
     4621 taaagtggtt gcagggcgtg aaaatataag tacgtttata tagaactaca atgcaataga
     4681 taattgatag cggtatggct gaatccagag aggaggaaaa ttttgactct gatgcacaaa
     4741 aggagcaaga atctggtacc aattccgttg aacctccttc cggccgggag gagttgcaga
     4801 agaagtacga tgagctgaac aatcggttca tccggcttgc agcggacttt gacaactaca
     4861 gaaaaagatc agacagggat gtcgagacca gggtgaaatt tgccatagag agatttgcag
     4921 tggaccttct tgaggtggtg gataatttcg accgggcgct ttctgctgat ccccattccg
     4981 cccgggaagg cctggagcag attcacaaac agttccaggc tatcctggaa cgtcatggta
     5041 tctgccccat cgaggccagt ggaaaaaaat tcaaccctga ggaacatgag gcggtctact
     5101 gtgaaccctc tggtgaagag gatggaatgg ttctcaaaga gatctgctgt gggttccgca
     5161 tgcacgacaa agtcatacga tatgcaaaag tggtcgtttc gaaaggaaag gagactgagt
     5221 gaacatggta aaagagaaga tactcggtat tgatctgggg accacatttt catgcatggc
     5281 catcatggag gccggcaaac ccattgtcat ccccaatgca gaaggacaga gaactacccc
     5341 ttcggtggtt ggattcacga aagacggtga gcgcctggta gggagccttg cgaagaggca
     5401 ggcagtcacg aatcccaccc gcacgataca gtccatcaag cgaaagatgg gcaccaccga
     5461 gaaggtgaag atcgacgaca agagctacac tcctcaggag atatcagcga tgatcctcca
     5521 gaagatgaag accgatgcag aagcatacct gggggagaag atcagaaaag cggttatcac
     5581 ggtcccggca tatttcaacg atgcccagcg ccaggccacc aaggatgcag gccagatcgc
     5641 gggtctcgag gttctgagga tcatcaatga accaacggcc agtgcgctcg cgtacggtat
     5701 tgacaaggag caggacgtca ctgtgctcgt ctatgacctc ggcggcggga cattcgatgt
     5761 ctcgatcctg accctcggag atggggtgtt cgaggtaaaa tccacatcgg gaaacaatca
     5821 cctgggaggg gacgacttcg acaacaggat catggactac ctcatcgagg agttccagaa
     5881 gaaggagggc atcaatctcc gcacggaccc gatcgcgata cagagactga gagatgctgc
     5941 cgagaacgcc aaggtcgagc tctcccagaa ggtgaagaca aacatcaacc tgccgtatat
     6001 cactacggga ccaagcggcc ccaagttcct ggatatcgac ctgaccaggg caaagctcga
     6061 acagttgatt ggcgacctgg tggagtcgac agtggggcct gtcaagcagg cactaagcga
     6121 cgcaaaactc acccccgacc agatcgacca tgttctcctc gtcggcgggt ctacgagggt
     6181 accgctcgtc caggagaccg tcaggaagat actcggcaaa gaacctgaca aaggtatcaa
     6241 tcctgacgaa tgtgtagggc tcggcgctgc cattcagggg gcggtcctct ctggggagac
     6301 gaaggatatc gtgctcctgg atgtcacacc actcaccctg gggatcgaga ccctcggcgg
     6361 gatcgcgacc aagctgatcg aaagaaatac caccatccca acccgcaagg gccagatttt
     6421 ctccactgcg gctgatggcc agacgagcgt ggagatccac gtcgtgcagg gcgagcgtgc
     6481 gcttgcgaag gataatttca ccctcggccg gttccagctg accggcatcc ccccggcacc
     6541 gcggggaatc ccgcagatcg aggtcacttt cgatatcgat gccaacggta tcatccatgt
     6601 ctctgcaaaa gacctcggca cagggaacga gcaggcgatc accatcaagg gtgacaggaa
     6661 actcaccgag gacgatatca agagaatgac cgaggatgca aagaagttcg aggcagaaga
     6721 cgccaagaaa aaagaagaga tcgaacttcg gaataatgcc gatatcgcaa ttttctctgc
     6781 cgaaaagatg ctgaaagagc aggggaacaa tatcgaaccc gctgacaggg agaaagttga
     6841 gacaggggtt gccgatctcc ggaaagtgct cgaggcagac aacagcgatg agatccgtgc
     6901 gaagatggat acgctgaccg aagcagtgta taccatcacc accaagatct accagaaagc
     6961 ccaggcggaa cgccaggcac agcaggcgga atccggggat aaggggggct ccaccacccc
     7021 gggtgacgaa aacgtcgtcg atgccgattt caaggttaaa gacgagtgag cccgatgagc
     7081 gagggtgatt actacgagat cctgggtgtc tccaggaacg cggaagagaa ggagattaag
     7141 aaggcctacc gcaacctggc ccggaagtat caccccgatg tctgcaagga gcctggcgca
     7201 gaggagcagt tcaagaagat caacgaggct tacagtgtcc tctccgattc acagaagcgt
     7261 gcgcagtacg accatcttgg tcacgacacc tataccagcg cctcgaaagg gtcgtacagc
     7321 gggacagggg gttttcatgg cggaggcttc agcgccgact tcagcgggtt cggcgacata
     7381 ttcgattttt ttgggggtgg gttctccggg tcgcggcaga cgggcccgca gtcgggttcc
     7441 gacctcctga tgaagatgca gatctcgctc gaggatgcag tctttggtgc agaccgcgag
     7501 atcgaggtga atcataccga ggcgtgcacc cactgtaatg gcaccgggag cgagagcaag
     7561 aagcttgtaa aatgcccaag gtgcgggggt tcgggccaga tgaggcagat gagccagaca
     7621 atctttggcc agttcgtgag gatgagcacc tgcaccgagt gccgcggacg gggaaggatc
     7681 ccggagaagc ggtgcacggt atgcaaggga tcggggcata cccaggtgca caggaaggtc
     7741 acggtgcaca tcccggccgg catcgacagc gggatgcggc tccggatgga agggtacggg
     7801 gaggccgggg actacggcgc accgaatggc gacctcttca tcgaggtcta cgtgcggccg
     7861 catgaccgtt tccgccggac cggtgaccac cttgagacag aagtggaggt aagcccggca
     7921 caggcagtag ttggctccat tgtggacgtg gagacaatcg agaagcggac cgtggaactg
     7981 accatccctg cggggatcca gcacaatacc gccctgaaga tcccgggaga aggggtcagg
     8041 cgcaggggga agcccggcga cctccttgtc agggtcaggg tggtgatccc aaagcagcca
     8101 aaagctgaga tccgaagcct ctacgagaag atactggaaa tggaggggca ccggatggct
     8161 gactcaagga aagggttctt ttcggggttc atggggaaga aataacaaat tcccctcgtt
     8221 ttctgcgtaa atccctcttc caatggtaat ttcactcaat ccctgggatt gagatcatca
     8281 ctctcatgac ctcttttatc ccctggtttt tttgccttca ccgtcctatt ggtgggcgtg
     8341 cacctcctcg ttgagctcgc cggagaccac ccgcagctgc cgttcgccga gcttgacatt
     8401 cttggacccg tgctcgagag aggcacccag gtcgccctgg tcgactgcca gcatgcagag
     8461 gagatcccac gtctcgcact tgcacattcg gtcatggagc acctgggctc ctgccccgct
     8521 gaaaagggcg cattttcacg catgttaaac gacctcgcga tatcgagtcc ccggccgttc
     8581 gcaggaaggg taaagaaggt ctacggcaac accatggatg cgtcccaggc ggagctcgaa
     8641 cggctcatcg ggtcgctcgt gcagggaaag gtggacctgc agaacccggc cgaggaattc
     8701 cgggcaatcg tggcagggtc caggtgctac cttggccgcg tcctgtggag gagcgacccg
     8761 cgacgatttg gagaccgccg tccaggtgac aggcccttct tccatccggg ggtcatgatg
     8821 ccccggatgg tgcgtgcgct gatgaatatc tcctgtgccc ggcaaggcga aaggctcctt
     8881 gaccctttct cgggcaccgg cgggatgatc atggaggcgg cccttctcgg cataattggg
     8941 atcggaagcg acattgaccc tgaaatggtc agggggagca ggatgaatgc acgggaaggc
     9001 gtgattctcc gtgccgacgg caggagactg ccgttccgtg acgaatcgtt cgatgcggtg
     9061 gtcaccgatc tcccctatgg gcagtctgtc tcaatcaggg cacgcagcct tgactcgctt
     9121 tatcacgatg cccttgggga gatcagccgc gtggttaaac cgggccgccg ggtggtcctg
     9181 gtcactcacc gggacatccg catgcgtgca ggcaatttca tgagggtgga gggatgctac
     9241 tcccagaggg tccacaggag cctgacgagg aacatactcg tccttgtaag agatgggtga
     9301 atataatggc atacctaagg actgacacca ttcaaatgga atgactgcga aaaattgagg
     9361 gggatcttct cagaatgcat tatgagatga aagagggaga agtccgtggc ggtggggtga
     9421 tatggacctg ctcacgcact gatccgggag tccgtgaccg gaacgaggat gctagcggca
     9481 cattcaccct ccctcacagg gaaggagcac tctatctcct tgcagtcgct gacggactgg
     9541 gaggtcaccc tgcaggggaa gtggcaagcg ccatcgctat ctccgccctg cggagtgccg
     9601 tgtcttcgac ggttgaggca ctcgagggca tggacgtctc ttccctgcag gcgatccttg
     9661 ccacggggtt ttcacacgcg aaccgcgagg tgatacgcta tgcagcgacg actccctgct
     9721 gccttggaat ggggaccacc ctcgttgcgg ctctcatgaa cggggaagga gctggcgtcg
     9781 tggggaacgt gggagacagc cgcgcctacc tctttggaga aggtaccgtg aggatcaccc
     9841 gggaccactc aagggtgcag gagatggtgg accaggggct gatatcgcag gagcaggctg
     9901 ggaatcatcc gttgaggcat attgtcacaa ggattatcgg gagaacggat gatatccctg
     9961 actttttccc tttccgcctc aaggacgatc tccttgtcct ctgctcggat ggactcgtcg
    10021 atggtgtcag cgagggggag ctgcagtccc tggctggcag gcatgggtcc cctgatatct
    10081 gcgagcgcct cgtggagtat gcgagaggga ggagccgtga taatatcacc atagtagccg
    10141 caggccgcag gcgaggcctg gcttttcacg gtgattgcat cctggcctga tcacaagaaa
    10201 aactggttat tttttggatc ctggtgcagc cgtcacgatc tggacggctt ccaccattgc
    10261 agggagcatc tcggccggga accccatcac catctccccg tcttcaatcc ctgagaactt
    10321 gcgtgagccg tcgcacccca gggaatagtt ggctttcccg gagaggtagg tctgggcgca
    10381 ggcatctgcg cagactgact ggataccaga aaactccgac gagatccttc cgcccaggga
    10441 aaagagagtc gcctgagcca atttcagcat tacccgggcc ggcgcaatga taaggacgac
    10501 ctgcggggag aaattggtct tctcaagggg ggcatagagg gttgcgactg tcacacccga
    10561 ctccacgtgg gggattcggt cgatcgtcct cttgcaggcc gcggtgctct cgaacttccc
    10621 aagcttatag tagaaatggc cattcttcag ggtctcggta aggggccgga gcccgagtgc
    10681 ccatgcaccg cccatgcact cgtggttctc gactttcgca tagaagactt ttccttctct
    10741 cctggccatg tccaccatct tgcagtggcg gaaattgccc tccagctgtt tcattccatc
    10801 ggggatatca tcgtggctgg tcgccatcct gattgcgaca ggggagtaat tgaggcccag
    10861 cgcttttttc agggcatcgg acgcatcttt gtaatccatt cgcttcatta tagtctcgtc
    10921 catttttgca tcacttccgt caattcctta aaaaagatgc tcgggctcat caaagataaa
    10981 actgctccta tgagtataaa atgcgttaca ggcgccgcaa cttgtgatcc actttcctga
    11041 agaagttctt tttggcatcg acgaagagcg cgggtcttgc agcccttgtg accacgatgc
    11101 ttccctccat cccaagctcg atggacttct gcccgtcgat gacgagactt gcgggcttcg
    11161 cgctctcgag gcgcacctcc acgctccgtt cattgctgat caggtggggg cgcgaggaga
    11221 gcatgtaggg tgcaaggggg acgagaagga atccctcgat gcgcggatcg acaatgggtc
    11281 ccccggcgct catggcatat gcggtggaac cggtgggtgt gctgatcagc agcccgtctg
    11341 ccctgaaccg ttcggcgggg atgccgtcta ccaccaccac aaaacggagc atcttggccg
    11401 ggcggttggt cacgatcagg gcttcgttca gtgcctgccc gagcaccacc ccttcccttg
    11461 cgagagagat tcgcatccgg ggctcgattg caaaccctgg ccgaagggac ttgataaacg
    11521 ccctcgcctc gcccggttcc aggtcggcga gaaagcctac ctctccccag ttgatcccga
    11581 ggacagggac ctgtttctcc atcttctgga cggaatgaag gatggtcccg tcaccaccta
    11641 ccaccaccac gagatcggac tgggaatccc atgtccacgc cgcatcaacg ccgagtgccc
    11701 gggcggtggc aggctcgaaa cagacctggt tttcgtcctg caggagtgca tctccgaggt
    11761 ccctcgtata ttcgagggcc ttctcgtcgt ctatccgtga caccatcacg atcttcattg
    11821 tgtggtccct acctcaggta ctcgatcacc ttgtggtgca ggacgccgtt tgttgccacc
    11881 atgcacctcc cgatggtcac ctcgtcgggg aagtggatgg gcatcccctc aaggtcgctg
    11941 accttacccc ctgcctcggt gcagatcagc atgccggcag ccgcgtccgt gaccctcaat
    12001 gtgccacgga tatcgataaa gccatctatc cgcccgcacc cgatgtagga gagttcgagt
    12061 gcggaggccc ccaggagacg ccaccggcgt atcttctggc ctatgtgcat caccctgcgg
    12121 gggtcaaatt tcctcccgta tatgctcatg gcgctctcat cgaggtttgt cgtccgggaa
    12181 gcggtcatcg gccgcccgtc cagccaggcg cccttcccgc gaatggcagt gaaagtctcc
    12241 ccgctcgcga ggtcttccac gaacccctgg agcacgcatc ccccctccgc ataggcgagg
    12301 gagagggcat agaaggggat gcctgcgact gcattgtagg tcccgtctat cgggtcaagg
    12361 aagatagtcc ctgcatcgcc ggagatctcc acctttccgg cctcttcgga gataagggtc
    12421 ctgcagaggg ggtggtcctg caggaaccgc agtacacagt cctctgccac ctggtcgatc
    12481 agcttggtag gagtgcgatc cgcacccatg cggacagtcc ggccgccctc atcgctaccc
    12541 accaggtcac ggattgcgcc ccgcacttca cgggcaagga gtgtacatgc gtctaaaaac
    12601 tccatggtgc ctgatccgac cgtttctgta ggagtattta tctgtagata taaagataca
    12661 tgttactgcg ttttcacgcg ggcgtgaacc ataatgaagg aacagacaga ggtaggaaag
    12721 ctcaaagaag ggcggtacat ggtaattgag gacgagccct gtaagatcct cgggatatcg
    12781 gtctcaaaac caggcaagca cggagccgcg aaggcccgaa tcgacgcggt gggtctcttt
    12841 gacggggtca agcgctccat tgtccagccg gtctctgcga agacctacgt gcctgtggtg
    12901 gagaggaaga gcgggcaggt cattaccatc agcgggaaca ccgttaccct tatggacatg
    12961 aaggaatacc acaacttcga gatcgaagtc cctgccgata agatggacag gatcgagatg
    13021 ggaaaagaga tccagtacat ggaagcgctg gggaagagga agctcgatct ggattgatac
    13081 cacctctgca ttcccgcgag cccttctttt caaccatttt tcccgctgaa acaccaaaag
    13141 aatcctcgaa cggtgactga acgggtcctg cctgtccggt tctcgacccc gcaaaaacta
    13201 aaagagcggc ttccatgagg aatacccgca gacttttcac gaggtttttt agtatttccc
    13261 gagataccgt tccgggactt taacctccat cctgtacgaa tataccatgt gcgtgtggac
    13321 cacgaagtag taggaccttg tggtggtccc ctcgtagaac ttctccaccc acggtcgcgg
    13381 atcgttctcc ttctccttct cccagagctc ggcatcgagg ccgccacgag gttcaatcct
    13441 ccgtacaagg gagtgatcgg ccgcgtcctt cacttctatc gagaaagacg ggaacacttc
    13501 agaagcagta aaactcgcaa tccctgcagc ttttgattca gtctctccaa aaaaagtcgt
    13561 cttccagggg tctacggtgt aatgcagctc ccagtagggg aacggcatgg agatcgtctg
    13621 ggtcgtccca cctcccttgg actcgatcga ggcgtacgtg agcagtctct ctggcaccgg
    13681 ggtctcttcg gggaggcggg taaaagtggg ggtggaggca gggctctgca gcgagacata
    13741 gccgaggctc tgcgaagtgc cgctccaggt cggggtcggg gtaggtgcct tcgatgcggg
    13801 cttttccggt gtggagggtg tcgcaacctc ctggtatccc ggtggaggtg acgttggccc
    13861 gggagacgat ccaggcagga aagggagggt gagatccggg gacttgccgg tgaggattgg
    13921 cttgactacg actgccatga ccaccaccac tccaaggacg acgaggagta tggccatgtc
    13981 cctcttctcc atggaaggat cttcgactgt ttgatattaa gaggttaccc gtaagggaac
    14041 catgcctacc tggtgcagag cgcagcggtc tcgccgtact ggagcacgtg accccgcatt
    14101 gctcccacga gatcggcctt gtttgcaccg ggggcaaggt caagcattgt gtcgaggccc
    14161 cagactttga aggtataccg atgggtgctc ccgcgtgggg gacacgggcc ggtatacccg
    14221 atacggccgt agtcgtttct tccctggaac gcacgcaccg ggctctccac aactccctct
    14281 tttggaaggc ccggggggat gatctccatc ggcgggatgt tccaggccag ccacggggag
    14341 aacgagcagc aggaagggat gaacgggttc accgccatga tggcaagcga ggctgaggcg
    14401 aggttgccga ggcgtatccg cggcgaggag tcgtcgccgt cacaggtgtg ggtgggcaaa
    14461 aactccagga agtcaagttg cacgacaagt ttctccatct ctctcaaccc aactgtacct
    14521 gttctgcatg catgaatgtg acggacttgc aggcgccggg caatgatggc catgacactc
    14581 tacgggtaca cggatggcgc gtcccgggga aaccccgggc ctgccgcatg cgcatttctc
    14641 ctgcgtgacc aggagggaag gctcctcgca aaggaggctt tggtcctcgg gaccaggacc
    14701 aacaacgagg ccgagtacca cgcggtgatc gaggcactca gaaaggcggg ttcctttgct
    14761 tcccgcaacg tcgtgatcta ttcggacagc cagctcgtgg tccggcagtt acggggcgaa
    14821 taccaggtca ggaagcctca cctgcgcgag ctgcacgggg tggcaaagga cctggagcga
    14881 agcttcctct cagtggaata ccgatcggtc gggagggacg acccctggat tacaatggcc
    14941 gacaggctct gcaacgaagc cctcgattcc gcaaatgcct gatattcgtg cagggattgc
    15001 caccatgatc aatatttaaa gccctttcag ctaatatata tagactactc aatcgcacaa
    15061 taacgaggat atccatgaca aatgaggccg gcaatacgcc agaggaatgc aacggggtct
    15121 gcgaagggtg ccccagcgcg cagcagtgcg acagcgacaa gaaggatccg aaggccaagg
    15181 gcctcccgcc aaaagcggag attgacgtgg atcacgtcat ccttgtcctc tcagggaagg
    15241 ggggggtggg caagtccacc gtggcagtca acctctcttt cgcgctctcg gcacagggat
    15301 acaatgtggg ggtcctggac ctggacatcc atggacccaa catcccgaag atgcttggat
    15361 tggaaggcca gcaccttgcc gccatgggga agaaaatcca gccgcttcgg gtcaccggga
    15421 acctctcggt ggtatccatg gcattcctcc tcccgagcat cagtacccct gtcatctggc
    15481 ggggaccgat gaagatgagc gcgatcagcc agtttttgaa cgatgtcgac tggggacacc
    15541 tcgactacct ggttgttgac cttcccccgg gaacaggcga cgaggcactc tccattgtcc
    15601 agcttgcacc caatgtgaga ggtgcggtga tcgtgaccac cccccaggag gtcgccacca
    15661 tggacgcgat gaaggctgca aagttcgtgg aacagctcaa catcccggtg ctcgggatcg
    15721 tggagaacat gagcgggatg atctgcccgc actgcaagaa ggagatagac ctcttcggaa
    15781 agggcggggg cgagaagatt gcagcagaac ttggtgtcgc gtaccttggc agcattcccc
    15841 ttgaccccga gatgcgcaaa gcaggtgacg agggcaagcc attcatcatc aggagcaagg
    15901 acagccccac ctggaagagc gtggacaagg tcatggaagc gctggtcagg gtggtcgaag
    15961 agtaaatgac gctgctcgag gtgcttaccg gagaggagaa acccctggat atttacgacg
    16021 acatcgtgcc ggtcctcgag gagatcaacc tttttcctgc ccgcgttgaa gggatttacc
    16081 gaaaggcgat gacctatgat gacgattcgc ttgaccggct ccgctttgca ttgttgcggc
    16141 tccagattca tgccgacatt caccggaacg aggacatgga gcgtgcccag gccatcaagt
    16201 atgcgtcggt ggtcctcgag aaggtcatct ttggttccct gatgatgggg cacgaagagc
    16261 tcgagcacga ctgaaggctt ctcatttttc ccgttctcct ttcaccactc cgaaaatccg
    16321 cgatcattct cttccctgaa caaagtatat ggcggcaggt tgcgcacctg atccatattc
    16381 agcggtgaaa gaatggagaa gtatgcattc gtggcccggg tcccggatca gcccggctcc
    16441 ctgcacaagg ccgccgagat catctcccgt catcagggaa acataaacag gatacagttt
    16501 gatcgccgga tagatccaca gacggtcttt tttgaggtta cttcaacccc tgaagcaaga
    16561 aaggagatac gcgaggggct ccttgacctg ggatatctcc agactgccct gaaacctctg
    16621 agctttctga aaatctcgat ccatatccct cacacccccg gagccctgct cgagttcctt
    16681 cggtgcacga ctgcgttgaa ggccaacatt gcccttatcg attttgatga tcgcgggagc
    16741 aaccccgatc ggttgaccgt gagcctgaac ctggaggata gttccgcagt atcatccctc
    16801 gttgaccagc tcaagacacg ttaccggatc gaggtcctgg aatacgacac gagcgggaaa
    16861 caccttgacg atgcggtctt ctacctcagg ttcgcccagg cgatacgcgg aatcgttggc
    16921 gaatcggagg atgcattcct tttgaggctc cttggggata tcaaccacat cgtgcaggaa
    16981 ctgatgaacc gcgggcagga cccaagactt gtcttcgaga gcatccttat gacgggggag
    17041 acgctccgga acacttcggg caccgggttc tacgccgagg tacagagtgt gcggcttgcc
    17101 gacgacctca cccttgtctg catccagccg ccctgcgggg gaaacgtgtt cctccttgac
    17161 actcctgcgg aatgcgtaat ggtcgacacg gcgtacggca tctactcgca cgacatcgcg
    17221 accgttatcc ggaccaccca gccgggagcc cttgaccggc tgtcacgtat cgttatcaca
    17281 catgcggatg cggatcactg cggggcggga ggtcactatc ccgttcccgc gctgatacac
    17341 ccgggaacga tggacatcat cagggtttcc aaccgggcgt acggttcaag gagccagtct
    17401 tcgatcctgg aagaggtgta cactaccctc atcaacctct tctcgcaatt ctgtccgcca
    17461 cgggacgcag tgctcctccc ctgtcaaggg gaggagaaaa ggggctgttt tcccgtcatt
    17521 gccggactcg aagtcggaaa gcacaggttc gatgtgctgg agggattggg gggccacctc
    17581 cacggtcagg tctacctcta ctccagcacc ctgggggtct tcttctccgc agatacggtg
    17641 atcaacttcg atcacctgac acccgacagg gcacgttaca acaccctggc cgtgaacctt
    17701 gtgacgtcgg tgaacgtgga cagcgggaaa gcaaaggagg agcgttccgc gatcctcgac
    17761 atagtgaggg ggaccgcagg tccattctac gggcagagag agagatgcct ggtgtgtgga
    17821 ggccacggcc ctgtctcggt cctggaaggg agaatacttt caccgtatgg aacaatggtt
    17881 cacatccccc cggcggcgga ggggtgcccc cgtgaacggc cctgaagacc aaggacagag
    17941 agcgcggcaa gcgacatgac cacgacgcca ttcctggtgc agtcttcggg atcgagtgag
    18001 aagcgtgggc tgtggagata aatctctccc agactgcccg tgccgaggcg gagatagcag
    18061 agcgggacag gtgggctgac ctccccgaac agcccgaagt cctcgctccc ggtgagaggc
    18121 gccatgcggt ggattcggtc ccttccgacg cacggagcga gtgcggagat gatacccgag
    18181 gtgagctccg gatcgttctc gatgggagac actgactcat caataagggt tatcaccggg
    18241 aggcggtctt ccggcatccc catgctcctt gcggagtgtt cgcagaccct tgcaatggaa
    18301 tcgaggatca ggtcgcggac tgccctggaa aagaagcgga tattcacctg gaggttcacc
    18361 tggtccggga tcgagttgtg cttggtgccc ccgtggacgg ctgccaccgt gatgagaccg
    18421 aactcccctg cgggcacttc tcttgtgatg atggtctgga actggaggat cagctgggca
    18481 gcaaggacga ttgggtcgcg gcagcggtca gggtgcgcgg catggccgcc gatgccacgc
    18541 acgatgatgt ccagcgattc tgatcccgcg gtgaagactc cttcgcgata ccccacgtgg
    18601 ccggcgggaa gttccgggga gacatggaag gaaagggcgc agtcaggcct gggaaaccgt
    18661 gtatagagcc cgtcatcgat catcccctgc gcacctgaac ctttctcttc agaaggctgt
    18721 gcaacgagga ccaccgtccc ggtccaatgg tcacgtattc ccgagaggta atgtgcagtc
    18781 cccgcgagca ccgtgcaatg caggtcatgc ccgcaggcat gcatgacccc gacctccttt
    18841 ccatcgggag ctactgccct ctcaaggctc gcataggaaa ggcccgtgtc ctcgtcgata
    18901 gggagtgcgt ccatgtcggc ccggatcatc accaccgggc ctatcccgtt ctccagcacg
    18961 ccgactacgc catgcccccc gatcccacgc gtaacgcgga accccgcgga gcccatctcc
    19021 gtcgcgataa cagtggacgt gcgggcttcc tgccccgaaa gttcggggtg tgcgtgaagg
    19081 tccctgtaca gggcgaccag ttccggaatg tggtcgcgaa ggtattcgtc gagcgattgc
    19141 atgaaatcgc agggggccgg tggactcatt ttgccttcct cctcattgca tttccgttcg
    19201 ctttcatcga gaggtagcgc cggacagaca tctccaggta ctcctcctct gtgtcaatcc
    19261 ccacgaactg cctccccagg gaccatgcgg caaggccggt agtggaactc ccgcagaaag
    19321 ggtcgagcac ccggtcccct tcagtggtgc aggccgcaac gatacgtgtg agaagggcaa
    19381 gtggtttctg ggtgggatgg gacccattgc ctttctccga ggcacgtgaa gtgggaatac
    19441 tccagacagt gcgcatctgc cgcccacggc gtttcagtgc atcctcgctc cagtccccgc
    19501 acttcaggtc ctggtagtta aagacatgtg ttcctttcct ggacttcctc gcccagacga
    19561 gagtttcgtg gctggcagta aaataacggc agctcaggtt tggtggggcg ttgggcttga
    19621 accagcagat gtcgtttaag acatgaaacc cttctctctg cagggcaaat ccgcaggcat
    19681 agatggaatg gtatgtcccg ctgatccaga tgctcccatc ctcacacatc actctcctgc
    19741 aggcctttat ccagtccctg tggaacaaga agtcctcctc gatgccgttg cttgcgtccc
    19801 aggcaccctt gtctaccggt gcccgccttc ccgactggca ggtaaagcct ccattggaga
    19861 ggttataggg tggatcggca aaaacgagat cgaacgctcc ttccggcatc tctgcaagta
    19921 tttcgaggca gtcaccatgg tacagggtga aatggccatc ctggtagtag ggtattggat
    19981 ccattcacag ttcccgggtc aattcgtggg cgtgtcccgg aatttttgca aagggatagg
    20041 tgcacatgtc cgaaacatgc ctgtatcacc atattctgtg acctggcgga gataaaagca
    20101 tagtctgtcc gggagcagga aacctttctt tcaccaacaa cttctatcgt ctcgaaatac
    20161 ctaattacac cgggaagcgc atggcggcac acagagtcca ggatatgatg cgcctcatgg
    20221 ccaggaggat cggaaggatc gcggaatccc tctctgatga tgaggttgag gagttcgtgg
    20281 aggagatact ccgtgcacgg cggatctacg tcatgggggc agggaggtcg ggactcgtgg
    20341 caaaggcgtt tgccatgcgg ctgatgcacc tcgggctctc ctccttcgtg gtgggggaga
    20401 ccatcacccc tgcaatgagc gtgggggatc tgatcgtcgt attctcgggg tcgggccgga
    20461 cgaagaccgt ggcagatatc gcggagactg ccaaggagat cggggggaga ctcgcgctca
    20521 tcacctcgaa ccgggactcg aggattggcc ggattgcgga cgttatcgtg atcatcgagc
    20581 gccacaggga cgaggtcaag gacgacgggg ccgagttcga gatcaggcag atgatggggg
    20641 agcacaagtc ctttgccccc ctcgggaccc tctttgagac cgcagccatg atctttgctg
    20701 acggcgttat ctcgcgcctt atggagatca cccagatcga cgagaaggca ttgaaggatc
    20761 ggcacgcgaa cattgaatga ggaagattag catggaaaag atgcggtgtg cagggaggtg
    20821 cctggggata gagatctcag gcatccgcaa gctattcgag gcggcgggac ctggttcgat
    20881 caacatgggc cttgggcagc cggatttcga cacccccggg catatcaagg aaggggccat
    20941 ccgcgcgatc cgcgagggga agaccgcgta tacctcgaac atggggatac cggaactgcg
    21001 cgaggcgctc tcctcgaagt tcaggaatga gaacgggctt tcttactccc ccgaccagat
    21061 cctggtcacg gcgggtgcga gcgaagccct ccacataatc atgcacgccc tcgtggaaga
    21121 gggcgaccgg gtgctgtatg cggacccggg ctttgtctcg tacggagccc tggccgtgct
    21181 cgccggcggg cggcccgaag gggtgcccct ggacagcaac cttcgcatcg acgtggaggc
    21241 ggcgaaaaag atgatggacg gtgcccgcct cttcgtcctg aactctccgg ggaaccccac
    21301 cggcgcggtg gagacaaagg agaacatcag ggcccttgtg gaatacgggg acgaccgggg
    21361 ggtgaccatc gtgagcgacg aggtatacga gcacttcatc tacggtgcca cgcactggag
    21421 cgccgctcgt ttcggggagg acgtgatcac ggtgaacgcg gccagcaaga catatgccat
    21481 gactggctgg aggctcggat accttgcagg gccgaaggag tacgtagagc agtgcctgaa
    21541 ggtgcaccag tactgccagg catgtgcgac atccatctcc cagtatgcgg cg
//
LOCUS       DAOQYR010000029        21206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_2862_length_21206_cov_8.369108, whole genome shotgun sequence.
ACCESSION   DAOQYR010000029 DAOQYR010000000
VERSION     DAOQYR010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 21206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21206
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2862_length_21206_cov_8.369108"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            355..987
                     /locus_tag="PKM87_05150"
     CDS             355..987
                     /locus_tag="PKM87_05150"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HNQ29490.1"
                     /translation="MARYPILILGLALACFMVVFMAGCTQPQGGDGKPTPTPTAPVIP
                     SQSFDQESNGKQVTAPAGSAFSVTLPENPTTGYSWNLVHSAGLTLLNDEFIPPSTQLV
                     GAGGTRLWLFSAVEKGNQSVHGEYRRPWVPAGTVVFQDLEGGFYGIVGDDGKDYLPLN
                     LGADYRVDGLRVSFESEPAGDVSTIYMWGTPVNLTFIETTDVYDLHVLVN"
     gene            complement(1087..1635)
                     /locus_tag="PKM87_05155"
     CDS             complement(1087..1635)
                     /locus_tag="PKM87_05155"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HNQ29491.1"
                     /translation="MSGGGVAAAEGLKGKKKSVLVCYASRYGSTKDIAEWIEEELIRL
                     GMAVDCRPAGRDVDPAAYDAAVLGSPLYMGKWLPEAREQVSRERGAFGRIPVAVFSVG
                     YSLKEPNEKALKGGEAALSDVRLFITPRDAAFFPGMVDPGRLTPADKAILTLAGISGG
                     DFRDKERVCSWARTLPAVLGLL"
     gene            1746..2228
                     /locus_tag="PKM87_05160"
     CDS             1746..2228
                     /locus_tag="PKM87_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2124 domain-containing protein"
                     /protein_id="HNQ29492.1"
                     /translation="MKQKEVFQGVPGMLRPFKEFIEKKGMKAGDLVVYYGCPGTCTPF
                     VELLGFSVRSLELAQVFVPYVDEAKAQKIALVPDIGMQAKGAAKIANPAAVVVMGGLS
                     MPNVPVTADQVKSAIEKYPDAAKIGVCFMNMFEKAGWLKSIDFDLLIDASIDPVNVWK
                     "
     gene            complement(2297..2785)
                     /locus_tag="PKM87_05165"
     CDS             complement(2297..2785)
                     /locus_tag="PKM87_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNQ29493.1"
                     /translation="MAGGKLKIVVFGAYNAGKSTFIQAIDPKARHVEAHTNEGDTTVA
                     LDYGRVEIHALQVYLFGTPGQERFEFVRQIISRGMDAAILLVDCSVATDEFTLSLYET
                     LSKMQIPLAVMLNKCDLPGSCPATVRSAFESSLVYEICASERALALPALTAFVGTLAG
                     EL"
     gene            3009..3488
                     /locus_tag="PKM87_05170"
     CDS             3009..3488
                     /locus_tag="PKM87_05170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNQ29494.1"
                     /translation="MTERKESLAGTRVTMETTLGTIEIELFLDMPVTAGNFASLVKKG
                     FYDGVIFHRVIDGFMIQGGDPTGTGRGGPGYTIKDEFVAGRRNERGTISMANAGPNTG
                     GSQFFINLVNNHYLDSKHPVFGRVVVGMDVVDKIAKVKKDRNDRPLQPVTITRATLA"
     gene            complement(3895..6087)
                     /locus_tag="PKM87_05175"
     CDS             complement(3895..6087)
                     /locus_tag="PKM87_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor EF-2"
                     /protein_id="HNQ29495.1"
                     /translation="MARGRKSIEKVTELMSDPVHIRNIGIVAHIDHGKTTLSDNLLAG
                     AGIISEELAGKQLFMDSDEEEQARGITIDASNVSMVHEYEGMEFLINMIDTPGHVDFG
                     GDVTRAMRAVDGAVVLVDAVEGTMPQTETVLRQALKEGVRPVLFINKVDRLINELKVD
                     EMEMQIRLGRVIDKVNKLIKGMNEEAYNAGWKLDASMGTVAFGSALYNWAVSVPFMKR
                     SGVSFKEVYESCRTGDMKNLAKRSPLCEVVLDMVVKHLPNPKDSQKRRIKVIWHGDAT
                     TPEGKAMLDCDPNGPATLMVTDISFDPHAGEVATGRLFSGRIKRGTELYVMGTAKKVN
                     RLQQVGIFMGPTRVEVEEIKAGNIAAVTGLKDASVGSTVTTLLDMTPFESLKHYSEPV
                     MTVAVEAKNMKDLPKLVEVLRQVAKEDPTLQININEETGEHLISGMGELHLEIVTGRI
                     KRDKGVEIVTSEPIVVYRETVTQKAGPVEGKSPNRHNRFYLELEVLPDAIVDLIKNGQ
                     VSMNQSQLERRDALIAAGMEKDEAKSVKDIYGNTMFIDMTKGVQYLNETMELIIEGLH
                     EALDGGPLADEKVQNLKLRLVDVKLHEDAIHRGPAQVIPAVRSAVKAGMLIAGDTLLE
                     PVQKIQITVPTDQMGNATSQIQGRRGQVFDIQSEGDTVTVVGKAPVAELFGFAGDIRS
                     ATEGRAMWSTEFVGFELVPSNLMKEVVTSIRKRKGLKEQMPTPADYLA"
     gene            complement(6119..6700)
                     /locus_tag="PKM87_05180"
     CDS             complement(6119..6700)
                     /locus_tag="PKM87_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNQ29496.1"
                     /translation="MTETEPATRLLFNKWDLGEVQVTDPSLVRYISLSPVIVPHSTGK
                     FTRQEFNKANMLIVERLINRLMQSEMNTGNKQLATRIVRDAFEIVHKKTKKNPVQVLV
                     EAIANTGQREETVRLKYGGINVPKSVDTAPIRRVNTSLYFLSEAVSKAAHKSKKPVAV
                     CLADELIAASKGDMKAYSVNKKEERERIAKSAR"
     gene            complement(6707..7135)
                     /locus_tag="PKM87_05185"
     CDS             complement(6707..7135)
                     /locus_tag="PKM87_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNQ29497.1"
                     /translation="MGKGKFAARKLLKDAKKFRWSDPKFVRSQGGVNLKSDPLEGAPQ
                     ARGIVLEKIGVEAKQPNSAIRKCVRVQLIKNGRQVTAFAVGDGAINFIDEHDEVEIEG
                     IGGRLGRSMGDIPGVRYVVTKVNNVSLHEMVIGRKEKPRR"
     gene            7304..7717
                     /locus_tag="PKM87_05190"
     CDS             7304..7717
                     /locus_tag="PKM87_05190"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNQ29498.1"
                     /translation="MISRIFKEVHFDASHRLLHYKGKCACLHGHRWKVEVWMEGQVDG
                     RTGILVDYNTIKNVIERYDHQIILNEQDPMVAAILAFHPVITTPGEPTSELLATLIAG
                     MLNEECERLGLTARVTRIRVWESPTCHAEVIYEGQ"
     gene            7704..8312
                     /locus_tag="PKM87_05195"
     CDS             7704..8312
                     /locus_tag="PKM87_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ29499.1"
                     /translation="MKVSEIFRSIQGEGIRQGRPCTFIRFAGCNLNCSWCDTPQARIG
                     GREMDAGEINARVRELGGTYLCITGGEPLLQGAPLLSLLSSFHSSGYMIDIETNGTLD
                     FSPFQALASICMDVKCPSSGEESDLSLLSRITTNDSVKFVVRDEEDCRYACDVLMEHP
                     VRGEVFFSPVEGSDARAIARFLIDEDLPARLQLQLHKILGVC"
     gene            8316..8987
                     /gene="queC"
                     /locus_tag="PKM87_05200"
     CDS             8316..8987
                     /gene="queC"
                     /locus_tag="PKM87_05200"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNQ29500.1"
                     /translation="MRAVCLLSGGMDSTTLAYLAREMGFRILALHFNYGQRTEKKERS
                     AARTIAALLEAEEFREVDLGYLSSFGASSLTDHSVPVDRYNPEGLDVPNTYVPFRNGN
                     LLSIATSYAEARGAEAIFIGVQSQDYSGYPDCRPAFIDAFQRVIDTGTRGETSIRLHT
                     PFIHMNKKEILEEGMRLKVPYEHTWSCYQDEDAACGACGACHFRKAAFSALGITDPIP
                     YRGEE"
     gene            8984..9493
                     /locus_tag="PKM87_05205"
     CDS             8984..9493
                     /locus_tag="PKM87_05205"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNQ29501.1"
                     /translation="MTDLYRGKRLWVDRKEYALPNGSKVEKVAVHPGNAVVILPFRAD
                     GSCYLIRQYRFVIGEYILEAPAGTLNPGEDPVTAAHRELIEETGFQAETLIPRGSIYT
                     TPGFSDEVLHLYEAHGLSPSCLYEKDDDEIIELIRVRSEDLPGMISDGRICDAKTISI
                     ICRCIGATR"
     gene            9497..9844
                     /locus_tag="PKM87_05210"
     CDS             9497..9844
                     /locus_tag="PKM87_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29502.1"
                     /translation="MLFCIPCLALALLPVFARCVDERGRLSGDYLINEHGCLSLECPA
                     CTLEEKSFPGEGGYSVTRVAFHTPEGDVDASPRFLTFLSQASCSHPAQQEEGKTPQQG
                     RGVRTVRLRFPGQ"
     gene            9991..10122
                     /locus_tag="PKM87_05215"
     CDS             9991..10122
                     /locus_tag="PKM87_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29503.1"
                     /translation="MYLCYAMEESSGGRYAAIAVALDPEFAGFIGVSTSGFSRAGDS"
     gene            10106..10375
                     /locus_tag="PKM87_05220"
     CDS             10106..10375
                     /locus_tag="PKM87_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29504.1"
                     /translation="MPETRNSGDLRRFLLSIDPDACTERMAPRNIWILHSPGDTVIPF
                     ADGQALYQVLPEPKSFFPFNGTHGLNEEADAWIPGECAQIYGPAR"
     gene            10413..11687
                     /gene="prf1"
                     /locus_tag="PKM87_05225"
     CDS             10413..11687
                     /gene="prf1"
                     /locus_tag="PKM87_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor aRF-1"
                     /protein_id="HNQ29505.1"
                     /translation="MTGTPEMDEGRRRYEFKKTLEKLMAKQGSGTELISLYIPPDKQI
                     HDVTAQLRDEFGQCANIKSKQTRTNVQSAISSILSRLKYYKNPPLHGMAVFCGTIQLQ
                     GDRTDLECTIIEPPEPLNLYMYRCSSAFELDPLKQMLEEKNVYGLLVLDRREAYWGFL
                     RGNRIEPIGGVTSTVPGKQRKGGQSSVRFQRLREIAINEFYTKIGERSSDIFLAEKDF
                     FERFKGVLIGGPSPTKEEFEAGQYLHHEIQKRITGLFDVAYTNESGLAELVDAAKDAL
                     KGMEVIKEKEFMNRFLKELVKENGLAAYGEESVRKNLDVGSVDILLLSGSLRKSRVSI
                     RCQACGHADERTIQLEPGKTVEDILASQTCRKCNGPLVVDQEVDIIEELTRLADQTST
                     RVEIISDDFEEGSILYTAFGGIAAILRYRTGF"
     gene            11691..13355
                     /gene="argS"
                     /locus_tag="PKM87_05230"
     CDS             11691..13355
                     /gene="argS"
                     /locus_tag="PKM87_05230"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNQ29506.1"
                     /translation="MLSTLYHTIEYALKEATGQEEVFLVEGGEHADLASTVAFGLAKV
                     RRKNPAMISRELAETLAAREDFGDIAVEALGPYLNFHFSREVIERALQDATRPGYGTH
                     PRREERVVLEHTSANPNGPLHVGHIRNTILGDTLGRCFRKAGYPLEVQYYVNDMGRQI
                     AIVVWGFCTIADTSLAGEKGDHHVARIYIAANRALEARPECVAEVDRLMQQVERGDQA
                     TVLLFRKAVTECLEGFRVTLQRLGARHDRFVWESDFIRNGDTDRVLSRISHMPECHDD
                     GKLWLDLSESGFEKEYVLRRSDGTSVYAARDLAYHTWKGRNFDRVIDVLGADHKLIGA
                     QLIATLRLLGEKCPEIVHFEFVSLPEGSMSTRAGKFVSADELLEEVERRAREEVTVRR
                     PELTETERLAIARSVALAAIRYDIARISPEKSTVFDWNEALNFERQSGPYIQYAHARA
                     CSILEKAGEFEECLALENEHEMALARHIIRFPMVIESVVRELKPHLLAAYARELADLF
                     NAFYHFEPVLKSEGITRRSRLTLVKATQNTLAEALQTLGIDALRTM"
     gene            13336..14229
                     /gene="twy1"
                     /locus_tag="PKM87_05235"
     CDS             13336..14229
                     /gene="twy1"
                     /locus_tag="PKM87_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-demethylwyosine synthase TYW1"
                     /protein_id="HNQ29507.1"
                     /translation="MHSGPCDALRRQGYQFFSPDSSAALKPCLWCKRALRGGDMCYKH
                     QFYGIASHRCVQMTPTLRCNQRCLFCWRSFEHEYPGERDLSPGEILSMVKPLQKKALS
                     GYKPSPYVTPERFAEALDPKHVAISLSGEPTLYPHLPELIDLLTREGFSTFLVTNGTR
                     PDVVARCRPFQTYVSLTAPDNETYRKVCRPMEDTWEAIQQSLSLLGSRRSAIRVTLVR
                     GYNDFSPDAYARMIQDSGASFVEVKGYMFLGYSRKRLERGNMPSFAHVKEFAEKIAAA
                     CDYEARDENPASRVVCLERIR"
     gene            14226..15812
                     /gene="sepS"
                     /locus_tag="PKM87_05240"
     CDS             14226..15812
                     /gene="sepS"
                     /locus_tag="PKM87_05240"
                     /EC_number="6.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043816 - phosphoserine-tRNA(Cys) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-phosphoserine--tRNA ligase"
                     /protein_id="HNQ29508.1"
                     /translation="MRFDPEEYRKKAREHFERAWHEGPSVLTPPSFGERYPRLAYCRA
                     AAHPIYEMVNRLRETYLSLGFTETCNPIIVDEQDVYRQFGPEASAVLDRVFYLGGLDR
                     PNQGIGREELEGISRITGKAVDPAAEERLREILQQYKKSAIDGDDLVHATSAALGIDD
                     GQTVEIYERVFSAFRELVPRSSRSTLRSHMTSGWFLTLSAIWEKYPLPLRLFSIDRCF
                     RREQEEGPTRLMSYHSASCVIAGEDITNEEGKAVSQALLSAFGYTDFRFRPDEKRSKY
                     YMPDSQTEVYARHPVHDWVEVATFGMYSPSALAEYGIGIPVMNLGLGVERLAMIAYNS
                     QDIRQLTYPQFHPRALTDLDIARAITVREEPSSMEGRRLARAIAATAREHATEPGPCA
                     FVAYDGPLLGNLVRVTVEEQESGAKLCGPACGNEVFVHQGSVLGVPDTPQWAAVRTEG
                     TPAGISFLEAVSARAAARIEEAARCGKSAHVQVKMAKLPSDINLRIDEHAMRFITDQK
                     KKVDVRGPVFLSVRSDVTGG"
     gene            16061..16561
                     /locus_tag="PKM87_05245"
     CDS             16061..16561
                     /locus_tag="PKM87_05245"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNQ29509.1"
                     /translation="MPSYRTAMQLRVVGVGRVRESFLNDGIQEYRVRISPYFHVELAD
                     VREGRHPRDPSPATRAGVLKEEGERLLSAARGSGVLVLLDIRGESWSSEDLAARLSSW
                     VLEGHSTVSFLIGGPFGVSEEVKNEATHVLSLSRMTYPHQMVRLILLEQLYRAARIHR
                     GDPYHK"
     gene            complement(16574..17695)
                     /locus_tag="PKM87_05250"
     CDS             complement(16574..17695)
                     /locus_tag="PKM87_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04084 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HNQ29510.1"
                     /translation="MFFHLILTDDCNLCCTYCRGKIFSEEAKWCGRNVLVDDLLPPDL
                     SIDLGDLYAFLRRDPDPCLTFYGGEPLLRPGLIMEIMDNAPPCRFMVQTNGILLRDLP
                     PEYLRRFETILVSLDGCAVVTDRHRGRGTYHRVMENIRYLEHTGFHGELIARMTVGDD
                     ADIAEEVKYLSRNPDHPFFSIHWQLDAEFSGDTGVRNFRKWLDESYNPGIRLLIDTWV
                     ERMAAGEGVARWYPFLQPVQDFLEGRKSPLRCGAGHSNYTIMTDGHIGPCPAMIGMTE
                     YYAGHISSAHPLSLPVIEVESECTKCPIRGFCGGRCLYSQIIRPWPDDMRVAVCEAVK
                     NLREGLLDALPRIRECIEMGLVLGEDFSHTRFNGCEIIP"
     gene            complement(17724..18587)
                     /locus_tag="PKM87_05255"
     CDS             complement(17724..18587)
                     /locus_tag="PKM87_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ29511.1"
                     /translation="MQLLPDPGGGRFRALLLATLAVSFILLQVCADNPTEVAMTLSKR
                     ADDFVAQERYMEAIDLYNEALALDPYSSATWNRLGRAEMSVGRYPDAVAAFQKSLDLD
                     PYNSAAWRNKGDALYAQGEYGAAIDAFDRALAVNANDLYALLQKGVCLQMMGRTAQAM
                     DTYNEVIRLAEREVRRNPNEAKYDATLWTNKADALTKLGRYHEAVEAYQNAIAINPKY
                     ERAVTGIEHVNETLFRARGSPELLNTPILPVETTPGEMPVPVSILSVPFAVGILAVLS
                     CLACQRRRKCG"
     gene            18770..19852
                     /locus_tag="PKM87_05260"
     CDS             18770..19852
                     /locus_tag="PKM87_05260"
                     /inference="COORDINATES: protein motif:HMM:NF019949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1724 domain-containing protein"
                     /protein_id="HNQ29512.1"
                     /translation="MDHPTNTGNETRPVPTIHLDAAEMAILLLLGRRPATLADLAHST
                     WLDPSQLYLVLGRLVHRWLAEQSGAHYGLTRSGMLLSSKIRAVVDAMGEGQGSDDEKR
                     ARRGESPPGPTRDTLERVEDLLKEAYWNPAHLSGASSAVIPVGETGNTRIDQELGDLS
                     AMAMTMEKFIDFFESHSLEGVPQEALDSLGDLLHAELTCDISLNFLHGWSQYRRIIEE
                     AQWVHGVSSFATPDIAEVIGKRVLGGVRVVLIITPDLADTILKEPYLEMARDLWRYRN
                     LKFRVSRIPVRIGLTVTDKALSFGLFTRSGQTYDAVYDLVSRSPESLLWGERLFAYYR
                     EHSDPLPGYMIKSALSSFLRGDIQEK"
     gene            complement(19931..>21206)
                     /locus_tag="PKM87_05265"
     CDS             complement(19931..>21206)
                     /locus_tag="PKM87_05265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ29513.1"
                     /translation="QAIQSNQSVRFEEKYGDKWYDSLICPILSDQGKVLQLVITARDI
                     TERIRIEEALREREGEIQNLIENTRDGIIITDEEGRVTRWNKGAELITGLTATDVRGV
                     PAWEIQARNVTTEWAGPDPLSWYKTLWSRLLADGNDQLFTGLFDGQIRTPNGDIKYIE
                     QSVFRIPSERGFRIGAIFRDVTDRKKEEQKIKEFADNLKRSNEDLELIVNIAAHDLQE
                     PLRGIVAYSQLLINRCRDEKDPLIEKYLKVIETSGLQMNRLLDDLRVYTRVRIHGNQF
                     SPVDTEKVLLNAQNNLELAIRENHAVIIHDPLPIVHGDAMQITQVFQNIIDNAIKFCG
                     DGVVPEIHISAESRQEAWQFAIRDNGIGIPEKYHNKLFILFERLHSRDTYPGTGLGLA
                     LCKKVIERHGGRIWVESEFGKGSTFYFTLPKE"
BASE COUNT         4399 a         6267 c         6008 g         4532 t
ORIGIN      
        1 aagtagtagg attaaacggt agattgtgcc ccccccggcc cacaggctat cgcggaccgg
       61 gggagggggc aggggagggg gattcccccc ctccccccaa acgaagaatt tattcactga
      121 aatcccgttt cacctgcctc ccctttgaga aaactataaa tcgcggcgac cgaagatcgc
      181 ctcggcgtcg attcgcgcat cccccgccat atttccctct gtattgataa tccccaaaac
      241 cgaatgcatc attcaataaa agtccatcaa tcacttcaaa cggattcgca gtcatcggaa
      301 agcgtttatc cggatgcatc catatgctgt cgtaccagag gtgattgcac aatcatggcg
      361 cgatatccaa tcctgatcct tgggctggca ctggcctgct tcatggtcgt attcatggcc
      421 gggtgcacac agccacaggg cggagacggg aaaccgactc cgacccctac tgcacccgtt
      481 atcccatcgc agtcgtttga ccaggaaagt aacggaaagc aggtgactgc ccccgctggc
      541 tcggcattct cggtcacgct gccggaaaac ccgactaccg gctattcgtg gaacctggtc
      601 cactcggccg ggctcacact gctgaatgac gagttcattc cgccttccac gcaactggtc
      661 ggagcaggcg ggaccaggtt atggttattc agcgcggtgg agaaggggaa ccagtctgtg
      721 cacggggagt accgtcgccc ctgggtgcct gcgggaaccg tggtcttcca ggacctggaa
      781 gggggtttct acggtattgt tggtgatgac gggaaagatt acctcccgct gaatctcggt
      841 gcggattaca gggtagacgg cctccgggta tccttcgaat cggagcctgc aggtgatgtc
      901 tctaccatct acatgtgggg gaccccagtg aacctcacgt tcatcgagac caccgatgtc
      961 tatgaccttc atgtgctggt gaattgaggg gggcgctcta cccagctcat ttttcaccgt
     1021 tgtcctcctc ggttggatgc acagagtatc gctgttccag ccagcaaccc gccgcctcat
     1081 cctcagttag aggagcccga ggactgcagg gagcgtccgc gcccatgaac agaccctctc
     1141 tttgtcacgg aagtcccccc ctgagatgcc tgcaagggtg agaattgcct tgtctgccgg
     1201 ggtcaaccgg ccgggatcca ccatacccgg gaaaaatgcc gcatccctgg gagtaatgaa
     1261 gagacggaca tccgagagtg cagcctcccc tcccttcagc gctttttcgt tcggctcctt
     1321 taacgagtag cccaccgaga aaaccgcgac gggaatccgc ccgaacgccc ccctctccct
     1381 tgatacctgt tccctggctt ccgggagcca cttgcccatg tagagggggc tcccgagcac
     1441 agccgcatcg taggccgctg gatcaacatc ccttccggcc ggacggcagt ccacggccat
     1501 cccgaggcgt atcagctcct cttcaatcca ttcggcaatg tctttcgttg acccgtacct
     1561 gctggcatag cagacaagga ccgacttttt ctttcccttc aggccttctg ccgctgccac
     1621 gcctccgcca gacatctctc actctcctcc ctcgtgccgg ccatccggtt cttccccggc
     1681 aggcttcgta cctatatatt catatgcacc gtgaatgtta ttcattctca taggagtaga
     1741 attgcatgaa gcagaaggaa gtgtttcagg gcgtcccggg catgctccgc ccgttcaagg
     1801 agttcatcga gaagaagggc atgaaggcgg gagacctggt ggtctactat ggctgtccag
     1861 ggacctgtac gccgtttgtc gagctccttg gcttttcggt gcgaagcctc gaacttgccc
     1921 aggtctttgt cccttacgtt gacgaggcca aggcgcagaa gatcgcgctc gtccccgaca
     1981 tcgggatgca ggcgaagggt gcagcgaaaa tcgcaaaccc tgccgcggtg gtggtcatgg
     2041 gcggcctctc gatgccgaac gttccggtca ctgcagacca ggtgaagtcc gcgattgaaa
     2101 agtacccgga cgctgcaaag ataggcgtct gtttcatgaa catgttcgaa aaggccggat
     2161 ggctcaaatc catcgacttt gacctcctga tcgatgcctc gatcgacccg gtcaacgtgt
     2221 ggaagtgaga agacccgtgc tcgtttttcc ccgggtgccg ttctccggag gggaacaggc
     2281 cccctgggaa ctctctttat aattctcctg ccagggtccc tacaaaagcc gtgagtgcgg
     2341 gcaatgccag ggcgcgctcg ctggcacaga tctcgtacac aagggaactc tcaaatgccg
     2401 atcgtacggt ggcgggacat gatccgggca ggtcgcactt gttcagcatc accgcgaggg
     2461 gtatttgcat cttcgagagc gtctcgtaga ggcttaacgt gaactcgtcg gtagcgacgc
     2521 tgcagtcgac gaggaggatc gccgcatcca tcccgcgcga gatgatctgg cggacaaact
     2581 cgaaccgctc ctggccgggt gtcccgaaga ggtacacctg cagtgcatgg atctcgaccc
     2641 tgccatagtc aagcgcaacg gtagtgtcgc cctcgttcgt gtgcgcttcc acgtgccgcg
     2701 ccttcgggtc gatcgcctgg atgaacgtcg acttccccgc attgtatgcc ccgaagacga
     2761 cgatcttcag ttttcctcct gccatcgaaa ggatctattc actccccggt ttttttaccg
     2821 tttctatgcg catctccagc gtatgcacca ggccccgggc acgaggagac gttgccgttc
     2881 ttttttatag cagcattccc gttattccct catgttcgca ccttcattcc ccctcaccct
     2941 cgaacctgcg ggtgctgcgc gacccagaac atccttctgt cttcacggta caataatact
     3001 gagtaactat gaccgagagg aaagaatccc ttgcagggac gcgcgtgacc atggagacca
     3061 cccttggcac catagagata gagctctttc tagacatgcc cgtgacagcg ggaaattttg
     3121 cctcgctcgt gaagaagggg ttctacgacg gggtcatctt ccaccgggtc atcgacggtt
     3181 tcatgatcca gggcggcgat ccgacgggta ccgggagggg agggccgggc tacactataa
     3241 aagacgaatt tgtggcaggg aggagaaatg aaaggggcac catctcgatg gccaacgccg
     3301 gcccgaacac aggaggaagc cagttcttca taaacctggt aaacaaccac taccttgaca
     3361 gcaagcaccc cgtattcgga cgcgtggtgg tcggaatgga tgtggtggat aagattgcaa
     3421 aggtaaagaa agacagaaac gatcgccccc tccagcctgt taccattacc cgggccacgc
     3481 tggcgtaagt gcccgcgtct ggcaagggtc aaaaccctgc cttcttttta aaaccacata
     3541 cgtccccgga tgggacacat gcattcgaga atgaaattgg cggaaccatt tttcatagta
     3601 accgctcatg accgccctgc cgtcgaatat aaacgatgaa aatcacgaag tgatttacat
     3661 tcaaagaacg tgtgtgggac tttaaacacg gccacctcgc cggccagatg cttctccatt
     3721 ttctgttctc gcctccaggg aagatggccg aaaacgatat cccccggagg ttacgtatta
     3781 tccatcttca tgggtggcac taaaaaaccg gttatgccag gaacgcacga taagtgggaa
     3841 agggatgcaa tgggagacgc tcacccacgc acctggataa aaaagagggg tttttcaggc
     3901 caggtaatca gccggtgtgg gcatctgctc cttgaggccc ttccgctttc tgatggaggt
     3961 cacgacctcc ttcatcaggt tgcttggaac gagttcaaaa cctacaaact cggtgctcca
     4021 catcgcccgc ccctctgtgg cggaacggat gtcccctgca aacccgaaga gctcggcgac
     4081 gggggccttg ccgacgaccg tgaccgtgtc cccttcgctc tggatatcga atacctgtcc
     4141 ccttctgccc tggatctggg aggtcgcatt ccccatctgg tctgtgggga ccgtgatctg
     4201 gatcttctgc acgggctcaa ggagcgtatc gcccgctatc agcatccctg ccttgactgc
     4261 ggacctgacg gcggggatga cctgtgcagg gcccctgtgg atggcatcct cgtggagttt
     4321 cacgtccacc agcctgagtt tcaggttctg caccttctca tcggcaagtg gaccgccgtc
     4381 gagtgcttca tggagacctt cgatgatcag ctccatggtc tcgttcaggt actggacacc
     4441 cttggtcatg tctatgaaca tggtgttacc gtagatgtct ttcacactct tggcctcgtc
     4501 tttttccatg ccggctgcga tcagggcgtc acgccgctcc agctgggact ggttcatgga
     4561 gacctgcccg ttcttgatca ggtcgacgat tgcgtccggc agaacctcga gctccagata
     4621 gaagcggttg tgccggttgg gggacttccc ctccaccggg cccgctttct gggtcaccgt
     4681 ctcccggtag accacgatcg gctcagaggt gacgatctcc acgcccttgt cccgcttgat
     4741 acgaccagtg acgatctcca ggtgcagctc tcccatccct gatataagat gctcgcctgt
     4801 ttcttcgttg atattgatct ggagagtggg atcctccttg gccacctggc ggagcacctc
     4861 gacaagtttc gggagatcct tcatgttctt ggcctccacc gcaaccgtca tcacaggctc
     4921 actgtagtgc ttcagggact caaacggagt catgtccagg agcgtggtca ccgtggaccc
     4981 tacagaagca tccttaagac cggtcaccgc cgcgatgttc ccggccttga tctcctccac
     5041 ctccaccctg gtgggcccca tgaagatccc cacctgctgc aggcgattga ccttcttcgc
     5101 ggtgcccatc acgtaaagct cagtaccgcg cttgatccgg cccgagaaga gccggccggt
     5161 ggccacttcc ccggcgtggg gatcgaacga tatatcagtg accatcaggg tggcagggcc
     5221 gttcgggtcg cagtcaagca tcgctttccc ttcgggggtg gtggcatccc cgtgccagat
     5281 aaccttgata cgccgcttct gcgagtcttt tgggttcggc aggtgcttga ccaccatgtc
     5341 cagcaccacc tcgcagaggg gactcctctt ggcaaggttc ttcatgtccc ctgtccggca
     5401 gctctcgtag acctccttga acgagacccc gctcctcttc ataaagggga ccgagactgc
     5461 ccagttatag agggctgacc caaacgccac ggttcccatg gatgcatcga gtttccaccc
     5521 ggcattgtag gcctcctcgt tcatcccctt aatcagcttg ttgaccttgt caatgaccct
     5581 gccgaggcgg atctgcatct ccatctcgtc caccttgagc tcgttgatca gccggtcaac
     5641 cttgttgata aagagaaccg ggcggacacc ctctttcaag gcctgccgga gcactgtctc
     5701 tgtctggggc atggtcccct ccaccgcgtc gaccagcacg actgcgccat cgaccgcccg
     5761 catggcacgg gtgacatctc ccccgaagtc aacgtggccg ggcgtgtcga tcatgttgat
     5821 cagaaactcc atcccttcat attcgtggac catggagacg ttgctcgcat cgatggtgat
     5881 gccgcgggcc tgctcctcct catccgagtc catgaagagc tgtttgccgg caagctcctc
     5941 ggatatgatc cctgcccccg caaggaggtt gtcagagagg gtggtcttgc catgatcgat
     6001 gtgggcaacg ataccgatat tccggatgtg taccgggtca ctcatcagtt cagtgacttt
     6061 ctcgattgat tttctgcctc gggccattaa aacaccacaa aaaagtgaat attgttcctt
     6121 atctggcgga cttcgcaata cgctcgcgtt cttccttctt gttcaccgag taagccttca
     6181 tgtcgccctt cgaggcagcg atcagctcgt ctgccaggca tacggctacc ggcttcttgc
     6241 tcttgtgggc tgccttggag actgcctcgg agaggaagta gagggacgtg ttgaccctcc
     6301 ggatgggtgc ggtgtccacc gacttcggga cattgatacc gccatacttc agcctgactg
     6361 tctcctcgcg ctggccggtg ttggcaatcg cctcgacgag gacctgcacg gggtttttct
     6421 tggtcttctt gtgaacgatc tcgaatgcgt cgcgcacgat gcgggtcgcg agctgcttgt
     6481 ttcccgtgtt catctcggac tgcatcagcc tgttgatgag gcgctccacg atcagcatgt
     6541 ttgccttgtt gaactcctgg cgggtgaatt ttccggtcga atgagggacg atcacggggc
     6601 tcaggctgat gtaccggaca aggctcgggt cggtcacctg cacctcgccg agatcccact
     6661 tgttgaagag gagccgggtt gcgggttctg tctctgtcat ttcagatcac ctgcgtggtt
     6721 tctccttgcg tccgatgacc atctcgtgga gggagacgtt gttgaccttg gtgacaacgt
     6781 aacggacccc ggggatgtct cccatggacc tccccagtct cccgcctatc ccctcgatct
     6841 cgacctcatc gtgttcgtcg atgaagttga tggccccatc gccgaccgcg aatgcagtga
     6901 cctgcctgcc gttcttgatc agctgcaccc tcacgcactt gcggatggca gagttcggct
     6961 gcttggcctc tacccctatc ttctcgagca cgatcccgcg ggcctgggga gcaccctcca
     7021 gggggtcgga cttgaggttg accccgccct ggctccgcac gaatttcggg tcgctccagc
     7081 ggaacttctt tgcgtccttg agcagttttc ttgctgcaaa tttaccctta cccatgaaat
     7141 accctcatgt acaagttcat ttgtggtgga ctcagaattc acccaatgtg gatatataaa
     7201 cgcgcggtgg cgacttatat attatggagg agtccccgat tgcctgtatt ggttgtatcg
     7261 cattgctaat aatattatat cccgttcccg ccaaacttct ctcatgatct cccgtatttt
     7321 caaggaggtg catttcgacg cgagccacag gctgctgcat tacaagggga aatgcgcctg
     7381 cctgcacggg caccggtgga aggtggaagt gtggatggag gggcaggtgg acgggcggac
     7441 cggcatcctg gttgactaca ataccataaa aaatgtcatc gagcggtacg atcaccagat
     7501 catactcaac gagcaggacc cgatggtcgc ggccattctt gcattccacc cggtcatcac
     7561 gacaccagga gagcccacga gcgaactttt ggcaaccctg atcgcgggga tgctcaacga
     7621 agagtgcgaa aggctgggtc tcacggcaag ggtgacccgt atccgcgtct gggaatcccc
     7681 cacctgccat gcagaagtga tatatgaagg tcagtgagat attccggagt atccagggag
     7741 aggggatacg ccaggggagg ccctgcacct tcatcaggtt cgccggatgc aacctcaact
     7801 gctcatggtg cgacaccccc caggcccgta tagggggaag ggagatggac gcaggcgaga
     7861 tcaatgcccg ggtgcgggaa ttgggcggca cctacctctg catcaccggg ggagagcctc
     7921 tcctccaggg tgcaccgctc ctctcccttc tctcatcctt ccattcctcc gggtacatga
     7981 ttgacatcga gaccaatggg actcttgact tctcgccctt tcaggcgctc gcgagcatct
     8041 gcatggacgt gaaatgccct tcttcgggtg aggagagcga cctttcactc ctctcccgca
     8101 tcaccacgaa cgactcggtc aagttcgtgg tccgggacga ggaggactgc aggtacgcct
     8161 gcgatgtgct catggagcat cccgtccgtg gggaggtctt tttctccccg gtggaaggat
     8221 cagacgcacg cgccatcgcc cgcttcctga ttgatgagga cctgccagcg cggctgcagt
     8281 tgcagctcca caagatcctg ggggtgtgct agcgcatgcg ggcagtgtgc ctgctctcag
     8341 gggggatgga ctcgacgacc ctggcctacc tggcaaggga gatgggcttt cgaatcctcg
     8401 cgctccactt caactatggc cagcgaacag agaagaagga gcgttccgct gcaaggacga
     8461 ttgcggcgct tcttgaagcg gaagagttcc gagaggtcga tctcggctat ctctcatcct
     8521 ttggagcgag cagcctgacg gatcactcgg tccctgtcga ccgctacaat cctgaaggtc
     8581 tcgatgttcc caacacctat gtcccgttcc ggaacggaaa cctcctctcg atcgccacga
     8641 gttacgcgga ggccaggggt gcagaggcaa tcttcatcgg tgtccagtcc caggactaca
     8701 gcgggtatcc cgattgccgg ccggcgttca tcgacgcgtt ccagagagtg atcgacacgg
     8761 ggacccgtgg cgaaacatcc atccggctcc atactccctt tatccatatg aacaagaagg
     8821 agatcctcga ggaggggatg cgcctgaagg tcccctacga gcatacctgg tcatgctacc
     8881 aggacgagga tgcagcctgc ggtgcctgcg gcgcctgcca tttccggaag gccgcgtttt
     8941 ctgcgctcgg gatcacggat cccattccgt accgggggga ggaatgaccg atctgtacag
     9001 gggaaagagg ctgtgggtcg acaggaagga gtacgccctt cccaacggct caaaagttga
     9061 gaaggtggcg gtgcacccgg ggaatgccgt ggtcatcctg ccattccggg cagacggttc
     9121 ctgctacctc atcaggcagt accggttcgt gataggggag tatatcctcg aagcgcccgc
     9181 agggaccctg aaccccggcg aggaccccgt caccgccgcg caccgcgagc tgatcgagga
     9241 gaccgggttc caggcagaga cgctcatccc caggggctct atttatacca cgccgggttt
     9301 ctctgacgag gttcttcacc tctacgaggc gcacggccta tccccctcct gcctttatga
     9361 aaaagacgac gacgagatca tcgaactcat cagggtaagg agtgaggatc tccccgggat
     9421 gataagtgac ggcaggatct gcgacgcgaa gacgatcagc atcatctgcc gctgtatcgg
     9481 ggcaacgcgg tgagacgtgc tcttttgtat cccctgcctt gcacttgccc tgctccctgt
     9541 gtttgcgcgg tgcgtggatg agcgcggcag gctatccggg gattatctga taaacgagca
     9601 tggctgcctc tccctggaat gcccggcatg caccttagaa gagaagtcct tcccaggaga
     9661 gggagggtat tcagtcaccc gggtggcatt ccataccccc gagggagatg tcgatgcttc
     9721 gccgcgtttc ctgacgttcc tgtcgcaggc ttcgtgctcg cacccggcgc aacaggaaga
     9781 aggaaagaca ccgcaacagg gcagaggcgt tcgcacggtc cggttacgct ttcctggtca
     9841 gtgacatgag gggaaacggc ggcgagaccg ggggataccc cctggacctt gagcgcgatt
     9901 accagaggtc cctttcccac gagtggcccc aatactctct ctcaatatgc gacatccagt
     9961 gcgccagggc atttctgcag gagaagactc atgtacctgt gttacgccat ggaggagagc
    10021 agcgggggac ggtacgcggc cattgctgtg gcactggacc cggagtttgc agggttcatc
    10081 ggtgtctcga cctcgggatt ctcccgtgcc ggagactcgt aactcaggcg atctccggcg
    10141 gttcctcctc tcaatcgacc ccgatgcctg tacagaacgc atggcacccc ggaacatatg
    10201 gatactccac tcgcccgggg atacggtcat ccccttcgcc gacgggcagg cattatacca
    10261 ggtgctgccc gagccgaaat cgttcttccc cttcaacggc acccacgggc tcaacgaaga
    10321 ggcagatgca tggatacctg gggaatgtgc gcaaatttat ggccccgcgc gttgattcat
    10381 gttgaagctt tgatatgtcc gcagagaggg agatgaccgg gaccccggag atggacgagg
    10441 gccgaaggcg ctatgagttc aagaagaccc ttgagaagct tatggccaag caggggagcg
    10501 gcaccgagct catatccctc tacatacctc cggacaagca gatccacgat gtgactgcgc
    10561 agctccggga tgagtttggc cagtgcgcga acatcaagag caagcagacg cgcaccaacg
    10621 tccagagcgc catatcctcg atcctctcgc ggctcaagta ttacaagaac cccccgcttc
    10681 atggcatggc ggtcttttgc gggaccatcc agctccaggg cgaccgcacc gacctggaat
    10741 gcaccatcat cgaacctccc gaaccactga acctctacat gtaccggtgc agttcggcat
    10801 tcgagctcga tcccctcaag cagatgctcg aggagaagaa cgtctacggg ctcctcgtgc
    10861 ttgaccggag agaagcatac tgggggttcc tccggggaaa ccggattgag ccgatcgggg
    10921 gtgtcacctc gactgttccg ggcaagcaga ggaagggagg ccagtcgagc gtccgtttcc
    10981 agcgtttaag ggagatcgcc atcaacgagt tctacacgaa gatcggtgag cggtcaagcg
    11041 acatcttcct tgccgagaag gacttcttcg aacgtttcaa gggcgtcctc atcggggggc
    11101 cgagccccac caaggaggaa ttcgaggccg gccagtacct ccatcacgag atccagaagc
    11161 ggatcaccgg actcttcgac gtggcctata cgaacgagag cggccttgca gaactcgtgg
    11221 atgcggcaaa ggacgccctg aaggggatgg aggtgatcaa ggagaaggag ttcatgaacc
    11281 ggttcttaaa agagcttgtg aaggagaacg gccttgctgc ctacggggag gagagtgtca
    11341 ggaaaaacct ggatgtcggc tctgtggata tccttctcct ctccggctcc ctccggaagt
    11401 ccagggtcag catcaggtgc caggcatgcg ggcacgctga cgaacggacc atccagctcg
    11461 agccggggaa gaccgtcgag gatatcctgg ccagccagac atgccggaag tgcaacgggc
    11521 ccctggttgt tgaccaggag gtcgatatca tcgaggaact gaccaggctc gccgaccaga
    11581 ccagcacgag ggtcgagatc atctccgatg atttcgagga ggggtcgatc ctttatactg
    11641 ccttcggggg aatcgcggct atcctccggt accggacggg gttctgaacc atgctgtcaa
    11701 cactctacca taccatcgag tatgccctca aagaggcgac cggccaggag gaggtgttcc
    11761 tggtcgaggg gggagaacac gcggatctcg caagcaccgt tgcattcggg ctcgcgaagg
    11821 tgaggcggaa aaacccggcc atgatctccc gggagctggc agagacactt gcagcgaggg
    11881 aggactttgg ggatatcgca gtcgaggccc ttggacccta cctgaatttc catttcagcc
    11941 gggaggtcat cgagcgggca ctccaggatg ccacgcgtcc cggctacggt acacacccgc
    12001 gcagggagga gcgtgtggtc ctcgagcaca ccagcgccaa cccaaacggc ccccttcacg
    12061 tcgggcacat caggaacacg atcctcggag acacgctggg caggtgtttc cggaaggcgg
    12121 ggtatcctct tgaagtccag tactatgtca atgacatggg ccgccagatc gccatcgtgg
    12181 tgtggggctt ttgcaccatt gcggacactt ccctggccgg tgagaagggc gaccaccatg
    12241 ttgccaggat ctatatcgcg gccaacaggg ccctcgaggc gaggcccgaa tgcgtcgccg
    12301 aggtggaccg cctgatgcag caggtcgaga gaggtgacca ggcgaccgtc ctcctcttca
    12361 ggaaggctgt cacggagtgc cttgaagggt tcagggtgac ccttcagcgg ctgggcgcca
    12421 ggcacgaccg tttcgtgtgg gagagcgact tcatccgcaa cggtgatacc gaccgtgtgc
    12481 tgtccaggat atcgcacatg ccagagtgcc atgatgatgg caagctctgg ctcgatctct
    12541 cggaatccgg cttcgagaaa gaatacgtgc tccggaggag cgacgggacc tccgtctatg
    12601 ctgcccggga ccttgcctac cacacatgga agggccgcaa cttcgatcgc gtgatcgatg
    12661 tcctgggggc tgatcacaag ctgattgggg cgcagctgat cgcaaccctc cgcctcctcg
    12721 gcgagaagtg ccctgaaatc gtccacttcg aattcgtctc tctcccggaa ggctccatga
    12781 gtacccgggc aggaaagttt gtctccgcag acgagcttct cgaagaggtg gaacggagag
    12841 cgagagagga ggtgaccgtc cggaggccgg agcttacgga gactgagcgc ctggcgatcg
    12901 cccgatcggt ggccctcgcc gccatccggt acgacattgc caggatatcc cccgagaaga
    12961 gcacggtctt tgactggaat gaagcgctta acttcgaacg ccagagcggc ccctacatcc
    13021 agtacgccca cgcacgtgcc tgcagcattc tcgagaaagc cggggagttc gaagagtgcc
    13081 tcgcgctcga gaacgagcac gagatggcac tcgcccggca tatcatccgg ttcccgatgg
    13141 tcatcgagag cgtcgtccgc gagctgaaac cgcacctcct ggcagcgtat gcaagagaac
    13201 tcgcggacct cttcaatgcc ttttaccact tcgagccggt gctgaaaagc gagggcatca
    13261 cccggcggtc ccgcctgaca ctggtgaagg cgactcagaa tacccttgca gaggcgctgc
    13321 agacacttgg catcgatgca ctcaggacca tgtgatgccc tgcgccgcca ggggtaccag
    13381 ttcttctccc ctgattcttc tgccgccctg aagccctgtc tctggtgcaa gagggcgctt
    13441 cgcgggggcg acatgtgtta caagcaccag ttctacggga tcgcgagtca ccgctgcgtc
    13501 cagatgaccc ccaccctccg gtgcaaccag cggtgcctct tctgctggag atcgttcgaa
    13561 cacgaatatc ccggggaacg ggatctttct cccggcgaaa tcctctcgat ggtaaagccc
    13621 ctccagaaga aggcgctctc gggatacaag ccctcgccgt atgtcacccc ggaacggttc
    13681 gccgaggccc tcgatccaaa gcacgtcgcg atatcgctct cgggggagcc tacgctctac
    13741 ccgcacctcc ccgagttgat cgacctcctc acacgcgagg gattctcgac gttccttgtc
    13801 accaacggta ccaggcccga tgtcgttgcc aggtgcaggc cgttccagac atatgtctcg
    13861 ctcaccgcgc ccgataatga gacctacagg aaggtctgcc ggcccatgga agacacatgg
    13921 gaggccattc agcagagcct ctcgctcctg ggctcgcgga ggtcagcaat ccgcgtcacg
    13981 ctggtgaggg gatacaacga tttctctccc gacgcgtatg cacggatgat ccaggactcg
    14041 ggcgcatctt tcgtggaggt gaagggatat atgtttctcg gatacagtag aaagagattg
    14101 gaaagaggga atatgccgag ctttgcccac gtaaaggagt ttgcagagaa gattgccgca
    14161 gcgtgtgact acgaggccag ggacgagaac cctgcaagcc gcgtagtctg cctggagagg
    14221 atccgatgag gtttgaccct gaagagtaca ggaaaaaggc aagggagcac ttcgagcggg
    14281 cgtggcacga gggcccctcg gtgctcaccc ctccttcttt cggtgaacgg tacccgaggc
    14341 tcgcctactg ccgggctgcc gcccacccca tctacgagat ggtgaatcgc ctcagggaga
    14401 cttacctctc cctcggcttt accgaaacct gcaaccccat aatcgtggac gagcaggatg
    14461 tgtaccgcca gttcgggccc gaggcgagcg cagtgctcga cagggtcttc taccttggcg
    14521 ggcttgatcg ccccaaccag ggaatcggcc gggaggaact ggagggcatc tcccgcatca
    14581 cgggaaaggc ggtcgatcct gcagcagaag agaggctccg tgagatactg cagcagtaca
    14641 agaagtccgc catcgatggc gacgacctcg tccatgccac gtcggccgca ctcgggatcg
    14701 acgacgggca gactgtcgag atctatgaac gcgtcttctc tgccttccgc gagcttgtcc
    14761 cccgttcctc ccggagcaca ctccgcagcc acatgaccag cggctggttc ctcaccctct
    14821 cggcgatctg ggagaaatat cccctcccat tgaggctctt ttcgatcgac cgctgtttcc
    14881 ggcgcgagca ggaggagggg ccgacgcgcc tcatgagcta ccactccgcc tcctgcgtga
    14941 tcgcggggga ggacatcacc aacgaggaag gcaaggctgt ctcccaggcc ctcctctctg
    15001 cgttcggata cacggacttc cgcttccgtc ccgatgagaa gcggtccaag tactacatgc
    15061 ctgactctca gaccgaggtc tatgcccgcc atcccgtcca cgactgggtg gaggtggcaa
    15121 ctttcgggat gtactcccca tccgcgctcg cggaatacgg gatcgggatc ccggtaatga
    15181 acctcggtct cggcgtggag cgtctcgcga tgattgcgta caactcccag gatatcaggc
    15241 agctcaccta tccccagttc cacccgcggg cgctcactga tctcgatatc gcccgggcaa
    15301 tcacggtccg ggaggagccg tcgtcgatgg aggggaggcg gcttgcgcgt gccattgctg
    15361 cgactgcaag ggaacatgcg acagagcccg ggccctgcgc ctttgtcgcc tatgacggac
    15421 cactcctcgg gaaccttgtc agggtgacag tcgaagagca ggagagcggc gcaaagctgt
    15481 gtggcccggc ttgcgggaac gaggtctttg tccaccaggg ttctgtgctc ggggtgcccg
    15541 acacccccca gtgggccgcg gtcaggacgg aagggactcc tgcggggatc tccttccttg
    15601 aagcggtcag tgcgcgtgcg gccgcccgca tcgaggaggc agcgcgctgc gggaaatccg
    15661 cacacgtgca ggtgaagatg gcgaagctcc caagcgacat caacctgcgc atcgatgagc
    15721 atgccatgag attcatcacc gaccagaaga agaaggtcga tgtccgcggc ccggtattcc
    15781 tctcggtccg gtcagatgtg acgggtgggt gatcctggag ggccccgccg ctcctccagc
    15841 gacctgctgt cgcgatacgt ggaagaggga acccgaaaag agcaagggga gttgccgggt
    15901 tcctctgata tcattgggtt tgagggctgc agagggaggc tcactctcgc ctccacacat
    15961 cttcatcttt tcatgggagc atataagagg cagccgtggg gggtgaaagt gaacctttcg
    16021 gacctgtccc ctggcaggat tgcgtctcct ttaaaatccc gtgccgtcct accgtaccgc
    16081 gatgcagttg cgggtcgtgg gggtagggag agtgagggag tctttcctca atgacgggat
    16141 acaggagtat cgtgtgagaa tctctccgta cttccatgtc gagcttgccg atgtgcgcga
    16201 gggcaggcat cctcgcgatc cctcccctgc cacacgtgcc ggggtgctca aagaggaagg
    16261 ggaaaggctg ctctcggctg cccggggaag cggggttctg gtgctcctag atatccgcgg
    16321 cgagtcctgg agcagtgagg acctcgcagc ccggctctcc tcttgggtcc tcgaaggcca
    16381 ctccaccgtc tcgtttctca tcggcggccc cttcggagtg tcagaggagg tcaaaaacga
    16441 ggccacgcat gttctctccc tctcccgcat gacctatccc caccagatgg tccgactcat
    16501 ccttctcgaa caactgtatc gcgcggccag gatccaccgc ggcgatcctt accataaata
    16561 ggcaaaaggg ggatcagggg atgatctcgc acccgttgaa gcgcgtatgg gagaagtcct
    16621 ccccaagaac caggcccatt tcgatgcatt cccttatgcg gggcagtgca tccagcagcc
    16681 cttcccgcag gttcttcaca gcctcgcaaa ccgcgaccct catatcatca ggccatggcc
    16741 tgatgatttg ggaataaagg caccttcccc cgcagaaccc ccgtatcggg cacttcgtgc
    16801 actcgctctc cacttcgatg acagggagag agaggggatg tgcacttgaa atatgccctg
    16861 cgtaatactc tgtcatcccg atcatcgccg ggcagggccc gatatgccca tcggtcatga
    16921 tggtgtagtt cgagtgcccg gcaccgcagc gaagcgggct cttcctccct tccaggaagt
    16981 cctggaccgg ctggaggaac gggtaccacc tggcaacccc ttcccctgcc gccatcctct
    17041 ccacccatgt atcgatcagc aggcgtatgc cagggttgta ggactcatcg agccattttc
    17101 ggaagtttcg cacccccgtg tcaccggaga actccgcgtc aagctgccag tgaatggaaa
    17161 agaatgggtg gtccgggttt ctcgagagat acttgacctc ctctgcgatg tctgcatcgt
    17221 ccccgactgt catcctggcg atcagttcac cgtgaaaacc ggtgtgctca aggtacctga
    17281 tgttctccat cacccggtgg taggtcccac gaccacggtg cctgtcggtg acaacggcac
    17341 agccgtcaag agagacgagt attgtctcaa aacgcctgag gtattccggc ggcaggtcac
    17401 ggagaagaat cccgttcgtc tggaccatga acctgcacgg cggtgcattg tccatgatct
    17461 ccatgataag gcctggccga agcaggggtt ccccgccata aaaagtgagg cagggatccg
    17521 ggtccctgcg aagaaatgca tacagatccc caaggtcgat gctgaggtca ggtgggagca
    17581 ggtcgtctac caggacgttc cttccacacc actttgcctc ctctgagaag atcttgcccc
    17641 ggcagtaggt gcagcaaaga ttgcagtcat cggtcagaat gagatggaag aacatggctg
    17701 gcgcgtggtg agggattata cgatcaaccg catttccggc ggcgctggca ggcaaggcag
    17761 gataatacgg ccagaatacc gacggcaaac ggcaccgaca gaatagagac agggaccggc
    17821 atctcccccg gcgttgtttc gacgggcaga atgggtgtgt tgagaagttc cggtgaaccc
    17881 cgtgccctga acagcgtctc gttcacgtgc tcaattcccg tgaccgcacg ctcgtacttg
    17941 ggattgatcg cgatggcgtt ctggtatgcc tcgactgcct catggtagcg gccgagcttt
    18001 gtgagggcgt cagccttgtt ggtccagagg gtggcatcgt acttcgcctc gttcggattc
    18061 cgcctcacct ccctctctgc aagccggatc acctcgttat aggtatccat tgcctgggca
    18121 gtcctgccca tcatctgaag gcagaccccc ttctggagca gggcgtacag gtcattggca
    18181 ttgacggcaa gcgcccgatc aaaggcatca atcgcagccc catactctcc ctgcgcatac
    18241 agggcatctc ccttgttcct ccatgccgct gagttatacg gatcgagatc aagcgacttc
    18301 tggaacgctg cgaccgcatc ggggtaccgc cccacgctca tctctgctct cccaagcctg
    18361 ttccaggtcg cgctgctgta gggatcgagg gcaagggcct cattatagag atcgatcgcc
    18421 tccatgtacc tctcctgggc tacgaagtca tcagccctct tggagagggt catcgccact
    18481 tcggtgggat tgtcggcaca tacctggagg agaatgaagg atactgcaag tgttgccagc
    18541 agcagtgcac ggaacctgcc accacccgga tcggggagga gttgcatacc cgttgatata
    18601 ttgcctccat gcgcttaata tgataacgaa tacacaaaaa aaccctttaa tacttttctg
    18661 gtctcaccaa tctcatgcat cgtattccat tctacgtatt ggataatgca atgtgatgca
    18721 ccgacttcac cattgtcgtc tttccccaag agttatccct gcacacccta tggaccaccc
    18781 gacgaataca gggaatgaaa cccgccctgt ccccacaata caccttgacg cggcagagat
    18841 ggccattttg ttgttacttg gccggcgccc tgcgacgctt gccgatcttg cacattcgac
    18901 atggctcgat ccatcgcagc tctacctggt cctcgggcgt ctcgtgcacc ggtggcttgc
    18961 agagcaatcg ggagcacatt acggccttac caggtctgga atgctcctct cttcaaagat
    19021 cagggcggtg gtggatgcaa tgggtgaagg ccagggatcg gatgacgaga agagagcgcg
    19081 caggggagag tcccctccag gtccaacaag ggataccctg gagagggtgg aagacctcct
    19141 gaaggaggca tattggaacc ctgcgcacct atcgggtgcc tccagtgcgg tcatccctgt
    19201 cggcgaaacc gggaatacca ggattgacca ggaactcggc gatctctctg caatggccat
    19261 gacgatggag aagttcatag atttcttcga gagccattca ctcgagggag tgccccagga
    19321 ggcactcgat tcccttgggg accttctgca cgccgaactt acctgtgata tctcgctcaa
    19381 tttcctgcat gggtggtccc agtatcgccg gattattgag gaggcccaat gggtccatgg
    19441 ggtatcctct tttgcaactc cggatatcgc cgaggttatc ggcaaaaggg tacttggagg
    19501 agtgagagtg gtgctgatca tcacccccga tttagcagat accatcctga aagagccata
    19561 cctcgagatg gccagggacc tctggagata ccggaacctg aagttcaggg tcagcaggat
    19621 accggtcagg attgggctta cagtgaccga caaggccctc tcttttggac ttttcaccag
    19681 gagcggacag acatacgatg cggtctacga cctggtcagc aggtcgcccg agtcactcct
    19741 gtggggagag aggctctttg cctattaccg ggaacactcg gaccccctcc cagggtatat
    19801 gatcaagtcc gccctgtcct ctttcctccg gggggatatc caggaaaagt gataggcttg
    19861 gcgaaaactg cacctttcgt tgtaacagag gatatgtgaa atttttttta aaaacccggt
    19921 atgagatagt tcactctttg ggcagggtga aataaaaagt agatcctttt ccaaattctg
    19981 actctaccca gatacgtccc ccatgccgtt cgataacctt tttgcagagt gcaagtccga
    20041 gccccgttcc tggatacgta tccctgctat gaagacgttc aaacaggatg aataacttgt
    20101 tgtggtattt ttccgggatg ccgattccat tatccctgat agcgaactgc caggcctcct
    20161 gccgggactc agccgagata tgaatctccg ggacaacccc atctccgcag aattttatgg
    20221 cgttatcaat aatattctga aaaacctgcg tgatctgcat cgcatctcca tgaacaattg
    20281 gaagcgggtc atgaatgata actgcatggt tctccctaat cgccaattcc agattatttt
    20341 gggcatttaa aagaactttt tccgtatcaa ccggactgaa ttgatttcca tgtatcctta
    20401 cacgggtata tacacgaagg tcgtctaaaa ggcggttcat ttgaagaccg gacgtttcaa
    20461 tgaccttcaa atacttttcg atcaggggat ccttttcatc cctgcagcgg ttgatgagaa
    20521 gctgagaata cgccacaata cctcgaagag gttcctgtaa atcatgagcc gcaatattca
    20581 caattagttc cagatcttca ttgctccgct tgagattatc ggcgaactct tttatttttt
    20641 gttcctcctt ttttcgatcg gttacatccc tgaaaatcgc gccaatacga aaaccgcgct
    20701 cgctaggaat cctgaataca ctttgctcga tatacttgat atctccatta ggggtacgga
    20761 tctgcccatc aaaaagccca gtgaagagct gatcgttgcc gtcagcgagt agtcgggacc
    20821 agagtgtctt gtaccaactc aatggatcgg ggccggccca ctccgtggta acattgcgtg
    20881 cctggatttc ccatgcaggt actcctctaa catctgtcgc ggtaaggccg gtaattagct
    20941 ctgctccctt attccacctg gtaacccgac cttcttcatc agtgatgata attccgtccc
    21001 gggtattctc tatcaagttt tgtatttctc cctcccgttc ccgtaatgct tcttcgatcc
    21061 ggatcctctc ggtgatatca cgagcggtaa tgacgagttg caggactttt ccttgatcgg
    21121 agagaatggg acaaataagc gaatcgtacc atttatctcc atatttctct tcgaatcgaa
    21181 cagactggtt cgattgaatt gcctgg
//
LOCUS       DAOQYR010000030        21007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_2895_length_21007_cov_8.834002, whole genome shotgun sequence.
ACCESSION   DAOQYR010000030 DAOQYR010000000
VERSION     DAOQYR010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 21007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21007
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2895_length_21007_cov_8.834002"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..331)
                     /locus_tag="PKM87_05270"
     CDS             complement(89..331)
                     /locus_tag="PKM87_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNQ29514.1"
                     /translation="MFEESHEGKHIFGTVKVGERGQIVIPKKAREVFSIQPGDTLLVL
                     GDEKRGIAIVKADAFHAMAMKMLKIFDSNPEEAPED"
     gene            complement(694..1110)
                     /locus_tag="PKM87_05275"
     CDS             complement(694..1110)
                     /locus_tag="PKM87_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HNQ29515.1"
                     /translation="MAAKNPFQTTLIAPCGMDCAICMAYLREKNHCDGCYSANCSCRR
                     YCTIATCEHVRDRYRHTCEKYPCRRLTQLDKRYRTKYGMSMVENLNTIREHGIRAFVR
                     IERERWTCKECGGVINVHTSRCASCGKERDDHNNFN"
     gene            1899..2543
                     /locus_tag="PKM87_05280"
     CDS             1899..2543
                     /locus_tag="PKM87_05280"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ29516.1"
                     /translation="MVDAERFWAHAASVYDDGIDYVFGNSMRQILLDALKRESNLGRT
                     VEFGCGTGYYTSLLAEMSGSVVATDIAEEMLERTRKRILGLPTVKVQKENCEKTTFPP
                     SSFDTVFFGLTFHMVDGPATLAEMYRILTPGGRLIIVTPVIDGLRISDILRTILRNKR
                     VFGLFRQPGTVLYTEQSLCDLVSHEGFLIQTTEHLQDPAHPGGFSGLYLTAGKA"
     gene            complement(2703..3137)
                     /locus_tag="PKM87_05285"
     CDS             complement(2703..3137)
                     /locus_tag="PKM87_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29517.1"
                     /translation="MKAILYAGILVMLAMLICGCTTTPPTGEKPPIAPKIPNILGNWS
                     GKMTGYVDGQGFTDYSGTQITMMVTGQKDRIFTGKFVYTEVSWEPTSFAGVIGPDGMT
                     LTIVEQEGGYSYGTFIGPDEIDLVYADNAVPFNVAIDSLRRD"
     gene            complement(3359..3661)
                     /locus_tag="PKM87_05290"
     CDS             complement(3359..3661)
                     /locus_tag="PKM87_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29518.1"
                     /translation="MRKNTYFIAALLLAIAFLVAPAAAGFYSPAKSAMPQISAPRTYL
                     SGFNVKDSSTFHQFQVQIQGMNLTPPPIPQMSLKSNDGKWDYNWGTARFNPGGYWL"
     gene            4065..4421
                     /locus_tag="PKM87_05295"
     CDS             4065..4421
                     /locus_tag="PKM87_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29519.1"
                     /translation="MPANSYTPSAYFDTPGYQPAAFFNEIGYTPSAYLNTPGYQPTAF
                     FDEMMYTPSAYFDTPGYQPAAFFNEIGYTPSAYFDTPGYQPTAFFDEMMYTPSAYFDT
                     PGYTPSIYFDFDGFIP"
     gene            complement(4561..5307)
                     /locus_tag="PKM87_05300"
     CDS             complement(4561..5307)
                     /locus_tag="PKM87_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HNQ29520.1"
                     /translation="MYQNMSPPLDAAIRSLAFSMGADLYGVADLGPARDFISRQGGER
                     IVRYPIAVAVGIRLSDDLVNLLAEHDPEGEILYRHTGYDVTNQLLDQIAVRLAGVLQR
                     EGYHAFPVPASRRTDDGEIRSVFSHKLAAHLAGLGWIGKSCLLVTPNHGPRVRWVSVL
                     TDAPLKPSGSPMKPRCGDCTACVDICPQHSFTGRMFDPDDPREARFNAAACDRYFEEL
                     QQSGGVAVCGLCLYVCPYGKRIESAHPGIK"
     gene            5416..5676
                     /locus_tag="PKM87_05305"
     CDS             5416..5676
                     /locus_tag="PKM87_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29521.1"
                     /translation="MKTQDIVRRLWDEAGRGNIAVWADGTITLVPKDYKGETGGKKPV
                     AILKPIALVNKYDFLDFALADEELLTTIEETIRAGGGTVSRG"
     gene            5904..7490
                     /locus_tag="PKM87_05310"
     CDS             5904..7490
                     /locus_tag="PKM87_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HNQ29522.1"
                     /translation="MNPIILFILAISPIILIFAGIVFLKQSGTLMAAVGWLLTVAIAI
                     FFFDTSPEVAVYASLGGILASFGISLMVLFTILQVTMMDLTGAIQRISASIKSIAAER
                     YEQIMILNVGFGSFLVSIGATPVTMLPPIMMALGFSPLASVALPCLGYDPLTSFSLLA
                     VPITLPAAIFGFDAVVMGSHVALFLPVISTGLSMGMLWVADGWEGIRKGWIPAVLAGL
                     SLGIFSIVFVNMLPASAIGLVGVFSGLLTIALLFLYRGVRGRRLIARKEDAPGQVVSP
                     SASAMMPLWKAVLPWVLLVVLVVIISIEDIQSGLYHLLGEAQVIHVVGNRAVDLRLMN
                     QAYFWVVVSTLVAAPFLVRSRNEGKKIIRLWLKRAWSPTLAAAVFFAIAYVMDWSGQC
                     IIDGTLTFVAGSTDLNMNAVIGLTLAMAFGIAFPIISPLLGLLGSFVSGSETSSNVMF
                     YGILQKATNVLSLDFEKVYAAHMVAGGIASGIAPAKIINAAAVIDQASIEGEVIKKSA
                     VVVILLALLTSALLVLSLVI"
     gene            complement(7575..8462)
                     /locus_tag="PKM87_05315"
     CDS             complement(7575..8462)
                     /locus_tag="PKM87_05315"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNQ29523.1"
                     /translation="MRWRTERRSTNIEDRRSSGIPMGRSSGIPGGKATGIGGLALILV
                     LVLAVAMGVDPGQIISGTDYASDSYGYQSPLTDTQQDELADHVALVLGDTESTWATIF
                     EDQLGKTYREPKLVLYTGYVDSACGQASAAVGPFYCPADEKVYLDLDFFYALQQEYHA
                     PGDFAQAYVISHEIGHHVQNLLGTIGYVDRKTRYLSDADANELSVALELQADCYAGVW
                     AKQAMYDDLILESGDLEEAMNAASAVGDDTLQMQYQGYVVPDSFTHGTSAQRVSWFRR
                     GMDAGLVRDCNCPGLPVIG"
     gene            complement(8797..9555)
                     /locus_tag="PKM87_05320"
     CDS             complement(8797..9555)
                     /locus_tag="PKM87_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ29524.1"
                     /translation="MIADFDRNQLAAEAVAAWAIAKKDMIIYYLKPNIIIAGLLFPLF
                     MFLAFAAGKNVPLGALIPGLISVTILFSSSTIEPVSIPIERRTKTFERLLSAPVSLHA
                     IVVGESLSGFLYSIGIALVPFVVGVAAFGNTISSPLALTVGMLLSALCFSTMGTLFAA
                     YPTESPGDIMSMLNLVRLPLIFVSGTFIPLDAMPPIGTAIAYFSPLTYGNDLIQCAYG
                     HPASFDPLVDVAMLGIFFVMFQLIAHRLYAHFND"
     gene            complement(9552..10505)
                     /locus_tag="PKM87_05325"
     CDS             complement(9552..10505)
                     /locus_tag="PKM87_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ29525.1"
                     /translation="MHAIEVKGLEKRFGEILALDRVSFAVETGEVFGYLGPNGAGKTT
                     TIRILTGITAPTGGSATIFGWDIVRDTIKARRDMGIVYETSNVYDDITAWQNMMFAGE
                     LYHVGKDDRESRGRELLEVFGLWDRRASRVKGFSKGMKRRLTLAMGLINDPKLLFLDE
                     PTSGLDVQSNLIIRDVLHSLTERGVTVFLTTHNIEEANQTCDRVAIINRGRIAAIDSP
                     ERLKKTIQSVQSVEVAFERGGARVLSDLAGLPMVSEARKVGDKVRLYTEDPGGLLREI
                     VTYADSHQDRVVSATTLGPTLEDVFIRLTGLRRPKNGGEEP"
     gene            complement(10678..11310)
                     /locus_tag="PKM87_05330"
     CDS             complement(10678..11310)
                     /locus_tag="PKM87_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ29526.1"
                     /translation="MRENSWKVANDYDKEATAIGWDAPAIVYALASPYTRPGQEIIDI
                     GIGTGLGSELFHCAGLRVVGLDISEEMLDACRAKGCVERLVRHDLTVFPYPFENDSFD
                     HAVSTGVFQFFENLSLIFQEVNRILKSGGIFVFVTGDRRQGEDAAAVIPPEHSRTTAP
                     VRMFRHSPREVRPWLEESGFRLFETREFSVWIDTARSDRFFLRAYLARKS"
     gene            complement(11523..12014)
                     /locus_tag="PKM87_05335"
     CDS             complement(11523..12014)
                     /locus_tag="PKM87_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ29527.1"
                     /translation="MAGSHYRGNAVDDPVVWKSSGNKFFKKGLYGIALRFYARAIQLN
                     PDFIEAWNNIGLSFLKLGRVDEARRCNEKVKELRMRSGNPLDPVGSATRVRTAASGSL
                     LNDNFWYALCIAVLVGISLTILTFDPGSTGPFQALAFIWGLSLMWFVIAYLLLQYVFH
                     RPK"
     gene            complement(12234..12671)
                     /locus_tag="PKM87_05340"
     CDS             complement(12234..12671)
                     /locus_tag="PKM87_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNQ29528.1"
                     /translation="MRTIRAEIRISAPDDVVWKALLASNAIPPEIGDAVHEMRIGQPF
                     RVMMSSGGRTMALTVTLLAASPFRYIRWKGCLWIPGFFDGEHSFEIIEESSECSRLIQ
                     HEKFSGVLVPFLSRTIDQTKEKFEEANAAVKSLAEQGYARRKP"
     gene            complement(12753..13244)
                     /locus_tag="PKM87_05345"
     CDS             complement(12753..13244)
                     /locus_tag="PKM87_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29529.1"
                     /translation="MNLNNQEPAGFDRSGKPMEWEYEHTQRGTLIIAAVLAVLLVIAV
                     SIVVFGPVWVTVIVGGIMVLMLAIFSTLTVSVRKDALRICFGPIRAFKKIWPICEIAS
                     VTTVTNPWYYGWGIRWTPYGPLYNIAGFEAVEVTLVSGKKFRIGTDQPDKLKRAIEKA
                     QYG"
     gene            complement(13249..13443)
                     /locus_tag="PKM87_05350"
     CDS             complement(13249..13443)
                     /locus_tag="PKM87_05350"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ29530.1"
                     /translation="MIRPVLSSPDACTSCGRCVIACRDGGYHAIALHDGKPEFDNARC
                     DGCSLCFHVCPESAIILTRD"
     gene            complement(13534..14448)
                     /gene="preA"
                     /locus_tag="PKM87_05355"
     CDS             complement(13534..14448)
                     /gene="preA"
                     /locus_tag="PKM87_05355"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HNQ29531.1"
                     /translation="MTADHPLATTLGPFHLENPFLLSSAPPTASYEQIRRAFSVGWAG
                     AVIKTIRPDGMVIEDVSPRFHAIKGSDAEVQGFENIELLSKKTVSYWTSQIPRLKQEF
                     PDRLLVASIMGTTDPEEWRNLAGQVEEAGADAIEMNVSCPHGMPEQGVGAAIGQNPAM
                     VRNLTRAVKDAADIPVIVKLTPNVTDVLLVADAASAGGADMISAINTVQCLSGIDLDS
                     FEPLPSVAGYSTYGGYSGPAVKPIGLRVVSQVASAVDLPVIGIGGISGWRDAAEYLLA
                     GASSVQVCTAVMWHGFGIVKEMQSGLSS"
     gene            complement(14614..15198)
                     /locus_tag="PKM87_05360"
     CDS             complement(14614..15198)
                     /locus_tag="PKM87_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HNQ29532.1"
                     /translation="MHELAAVWTERRGLAWIAATAFLYAMILIPFNQLDLHVAGISIR
                     PAAALPVLFGILFGPAAAWGLAIGNIPGDFYGSWSPMSIFGFLVNFLSPYLSYLLWHR
                     LMRCHEIRVDKESTGIFLLVSFVVILACMLLLAACGTIFFGRPFESKFVSYFGNNILW
                     ALTVGLVLFWLALEPAVRNEYVYGREWARRRTIQ"
     gene            complement(15581..16159)
                     /locus_tag="PKM87_05365"
     CDS             complement(15581..16159)
                     /locus_tag="PKM87_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29533.1"
                     /translation="MNVVFSALAIAFGVQYFLSSVLGLAEGDPFSLWRITSAAFSMAG
                     CGLGLFWVISGVKIMDGLDDIRSASRDRKAPVPGEVTICGIIRMIAHYLEDMKTIRTM
                     ICICTLGGFCFLALEIFSSIEFFSFCLTSGTITLGNARLIPPALPALGIAFVSLLSSL
                     YFWRFSNAWDVREGEISRAEQILADTLAGYSR"
     gene            complement(16321..17064)
                     /locus_tag="PKM87_05370"
     CDS             complement(16321..17064)
                     /locus_tag="PKM87_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ29534.1"
                     /translation="MLVTFLGTNGWYDTLTGNTCSVLVQSKDYNVILDAGNGIAKADQ
                     YLDQELPTFLLISHCHLDHIAGLHTLNKFRFSLPLHICGQKGISRELHSIVREPFTVP
                     FDHLPFPVRFIELVEGTHEFPFSIECRQLVHTAPCVGYALTLDGKKIAYCTDTGFCAN
                     AVLLARDADLLITECGLKPGETSPEWPHLNPGDAIRIAREARAKRLALVHFAAHTYTT
                     LEERFEIERMYEEECPGLVAATDGLAIEV"
     gene            complement(17286..17828)
                     /locus_tag="PKM87_05375"
     CDS             complement(17286..17828)
                     /locus_tag="PKM87_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29535.1"
                     /translation="MSTMADEPHSGGGKDERPPAPPTEKKIPRFSLIAGAVIVILVIL
                     IIAAFLTLSVQADLPPEPGTVYPYITTYAVLIPDGLPIRIAGTPILVLTSGDELIMKI
                     GDQNEKFVAGQTKTISERRAEFRALGIPLLSTNYLIEATYRGRSGPNADFFLIVRTSR
                     QVPSFLIERILPAEIQARPA"
     gene            complement(17965..19830)
                     /locus_tag="PKM87_05380"
     CDS             complement(17965..19830)
                     /locus_tag="PKM87_05380"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNQ29536.1"
                     /translation="MYGWAGATLRIDLTTSSIERRALDAGRARKFLGGRGFNSRVLWE
                     EVAPGTDPLGPGNVLCYAPGMLTGTPLALTGRVSVSTLSPLGGILGDGSGGGEFAWQL
                     KRAGYDQIVVKGRSPYPCVLSIDDERVEIRDARSLWGLDTWEATDRLRQDLGDDTSVA
                     CIGPAGERLVRFASTIIDKYASAAIGSGAVAGSKNLKAIAVRGSNPPRLADPEGFRDL
                     AKIDREIFRNDPFFRNTVSKYGSIIGVEDWGPGFRNSQRRWTGADVPPALRKDAWKEY
                     EVGHTGCHGCPLRCKNIIRIPEGPRKGEVGEGLEYEAIHWLGINSGILDPIAIMEMSN
                     YADRQGMDVIGLGSVVGFAKELFEKGIISLEDTGGISLDWEDSESQRLLITMTAERKG
                     FGDLLALGMAGLAERLGKDAARYCFDVKGVTRGVYPSGIYSLAHATSTRGADHLRGRT
                     WAGGENEEGLYRDLVQSGYIPTDPAGALRVGERATTLADALGRCKGAVNSWNSSVPLV
                     RKFPLWGGAIRLIRAATGIEYTPRELEDALDRIYLQEMSFNVRQGMTRADDRMPQRPE
                     VAGTSKGKEDLARHQVLLDAYYSLHGCTPLTGIPNRTELERLGLRDVADSLHMEN"
     gene            complement(19917..20765)
                     /locus_tag="PKM87_05385"
     CDS             complement(19917..20765)
                     /locus_tag="PKM87_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNQ29537.1"
                     /translation="MTGTGRPVVLVHGWKSHPGIWDRLVEALDKDAIPSWTFDHSRVR
                     GAAVESLAALLQEFIRDQRADTGYCGPIDMVCHSMGTGIARYLIEVLDKNDRCEKVRL
                     LVGLGPPNNGSCLAELFFDPVHSPRILKTLGGIFVPRRFNPADDIIVQQFRPGSEAMA
                     ALRSAGIREDIRYRFILTGNCDADPEFFPPFHGKTWVMTANGWETTYAGDGIVPHADS
                     WLQGAEYDILPLDPSSLARSPDLYCHLHLPRNPDVIGKILDSLSDPDTCAGTGCPECL
                     AMYRHL"
     gene            complement(20853..>21007)
                     /locus_tag="PKM87_05390"
     CDS             complement(20853..>21007)
                     /locus_tag="PKM87_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNQ29538.1"
                     /translation="WLLKEASRMHTGEVFSFVMERKRNMPRTALRYAIELMPKELKAE
                     AMKKDW"
BASE COUNT         4704 a         5836 c         5696 g         4771 t
ORIGIN      
        1 ggacatactg cagggtgata ctctcctcac ccttggggct cccggagatg atgcatatct
       61 tcatactagt cttcccccag aaccaatcct agtcctcggg ggcctcctct gggttgctgt
      121 cgaagatctt cagcatcttc atagccatgg cgtgaaaagc atccgccttg acgatggcaa
      181 tgcccctctt ctcatctccc agcacaagaa gcgtatcgcc aggttggatg gagaagacct
      241 cccgggcttt cttcgggatg acgatttgcc ccctctcacc tacctttacg gtgccgaaga
      301 tgtgtttccc ttcatggctc tcttcaaaca tgacttttca cactaatcat atctttcata
      361 tattcgtatt tgtatgatta atatgataaa tgtgttggcg ggagatcgag agaggacttt
      421 cccttccgcc ctgacgagcg aaggcaggtt cggccttcct ttatcccggc cattcatcaa
      481 ggcaaaccat tccattcggg atgaaaatga ccatcaggaa aagatacaaa gagattagag
      541 tttatcccag gtgcagtctt aacatctggg acatggaggt aaatcatcag tgtcattgtc
      601 aaggagaatc acctctctgt atgttcgaca acggtacatc ccttttcctg gtttatcgcc
      661 acacgtagat cccgtgatat atcccaacta tcctcaatta aaattattgt gatcatcccg
      721 ctcctttcca catgacgcac accgcgacgt gtgcacattg atcacacccc cgcattcctt
      781 acaggtccag cgttcccgct cgatcctcac aaaagcccgg atgccatgct ccctgattgt
      841 attgagattc tcgaccatgc tcattccata cttcgtccgg taccgcttgt ccagttgtgt
      901 gagccgtcgg cagggatact tctcgcaggt atgacggtac cggtcccgaa catgctcgca
      961 ggtcgcgata gtgcagtacc tcctgcatga acagttcgcg gaatagcacc catcgcagtg
     1021 gttcttctcc cggagataag ccatgcagat ggcacagtcc atcccgcagg gtgcgataag
     1081 cgtggtctga aaagggttct tcgctgccat cagatctgct tctcgcttca ggcattatta
     1141 aaggatttac ccgcttgttc cccagtttcc aggtagttgt gcatccgggt ccctaaacct
     1201 tttccgatat ttccgggatg gattaaaatg gtgatctcag cgggagtggg ggaattcagg
     1261 gactgttttg tgcgagagga gcatcccgaa cctggcaaag ggtcttaccc acatactggc
     1321 tgccaccgcg ggatatcccc ggaacatctg catcatcaga aaacaggaag gatccacccc
     1381 aggatcatag gtgtcgcaag caaataccac agatatgcga gaggaaaaaa aagagtccaa
     1441 gaacccatca ggtcggcccg gccggactca tctcatcgtc agggagtgcg acgagcgtca
     1501 ttatctccat ccggtccagc agtttcaggg ccaggtgcat cgcgactttc tcatcagggg
     1561 cttcaaagat cgcgacggca tcatatcccc ccgggagtcc ggtagatctc cctgtattta
     1621 acctttcact ttgtatcggc ctcaatatcc ctcatgttct cggcaaccac atctgtcgag
     1681 aggtccttct tgaacttcgc cagtgcgata aaatgaatca ttcacctcat cccttattgc
     1741 gggcacttca gcatatgaag ggggaaattt agatgtgggt cattggaggc agagatgagt
     1801 gttcatcacc cattttcact gccctcgcgt tacacgcgtt ggccagagtg gcccggtaac
     1861 cccaaagatt caagattttc tgtgcccacg ttcctgtaat ggttgatgcg gagagattct
     1921 gggctcacgc ggccagcgtc tacgatgatg gaatagatta cgtttttggg aattccatgc
     1981 ggcagatcct cctggatgcc ttgaagcgag agtcaaacct tggcaggacc gtagagtttg
     2041 gctgtggtac cggatattat acatcgctcc ttgcagaaat gtccgggagt gtagttgcga
     2101 ccgacatcgc cgaagagatg ctcgaacgga cgcggaagcg gatcctcggc cttcccacag
     2161 tgaaagtgca gaaagagaac tgcgaaaaga ctacctttcc tccctcatcc tttgataccg
     2221 tattttttgg tcttaccttc catatggtcg acggcccggc gacccttgcc gagatgtacc
     2281 gcatccttac tcctggcgga aggcttatca tagtcacacc tgtcatcgat ggtctccgca
     2341 tctcagatat cctgcgcact atccttcgta ataaaagagt gtttgggcta ttcaggcagc
     2401 cgggcactgt cctgtataca gaacagtcgc tgtgcgacct tgtcagtcat gaaggatttt
     2461 tgattcagac aacagagcat ttacaggatc cggctcatcc cgggggattt tcagggctct
     2521 atctcactgc ggggaaggca taacatctcc cggtgcattt cccggcagtc actgaccctc
     2581 caggcatttc gttctcagaa ataatgagtt gggtattacc agccatcgct ctgcaaaaaa
     2641 ggatgtgaaa acccgggaaa gaatgacagg tgtgcgtctt tttaaaaaaa gcgatggatt
     2701 tttcaatccc tccggagaga atcgatcgcc acattgaaag gtacggcatt atctgcatat
     2761 accagatcga tctcgtccgg gccgataaat gtgccataac tgtatccccc ctcctgttcg
     2821 acgatggtga gggtcattcc gtcaggacca atcacccctg caaaactcgt aggctcccag
     2881 ctcacttctg tgtatacgaa ttttcccgtg aatatccggt ctttctgccc tgtgaccatc
     2941 atcgttattt gcgtgccgga atagtcagta aatccctggc catccacata tccagtcatt
     3001 ttgccggacc agttgccaag gatgtttgga atctttgggg ctatcggggg tttttctcca
     3061 gtgggcggcg tggttgtgca tccgcaaatg agcatcgcca gcatcacaag aatacctgca
     3121 tagagaattg ctttcatgat ccatggattc caatgtaaaa agagataaat catatctatc
     3181 tcctctggag atattactgc tcaccattct ccggagcacc cttcatcaaa gagaacagga
     3241 ttcccgcaga atgtttttcc cttggaatgt cattccaaat tgatgtgtgc aatggctttt
     3301 acgaattccc tcattaaaaa tagggaggtt cagtgttccc tgctccagta tcccattctt
     3361 acagccaata accgccggga ttgaatcgtg cggttcccca gttatagtcc catttcccat
     3421 catttgattt cagggacatc tgaggtatgg gtgggggtgt caggttcatt ccctggatct
     3481 gcacctggaa ctggtggaat gtcgatgaat ccttcacgtt aaacccggac agataggtcc
     3541 tcggcgcaga gatctgcggc atggcgctct ttgcggggga gtagaatcca gctgcagcag
     3601 gagccacgag aaacgcaatc gccagcagga gggcggcaat gaagtacgta ttctttctca
     3661 tgagagaaag aaatggcaga ccgattatat aaattttgag attgaagacc atagataatc
     3721 ggacaaagcc ctacaaacag gggatctctc tctcccggat tgaattatat cagcatgcga
     3781 cttgagtggg aaattttaga ggagagatca aaaccgacga agtgaagggt catgcgcaga
     3841 aaattctgac actgttccac tccaatcctc cttgcattta ctgcgtctca tacagtccat
     3901 caattggtgg aggtttgttc cttccggcat cagattttta taaaggagcg ttgcaaggtt
     3961 gcactatgaa aaagttcttc tctgtcgttt gtatcctcgt ggtgatatgt tgcctcattt
     4021 ctgtatcctg tgtatctgga gaatattctg gatcggacat attcatgcca gccaattcgt
     4081 atacgccatc ggcatatttc gatacccccg ggtatcaacc cgcggcattc tttaatgaga
     4141 tcgggtatac gccatcggcg tatctcaaca ctcccgggta tcagccgacg gcattctttg
     4201 atgagatgat gtatactccg tcagcgtatt tcgacactcc cgggtatcaa cccgcggcat
     4261 tctttaatga gatcgggtat acaccatcgg cttatttcga cactcccggg tatcagccga
     4321 cggcattctt tgatgagatg atgtatactc cgtcagcgta tttcgacact cccgggtata
     4381 ctccctcgat ctactttgat tttgacggct tcataccctg atcgtcagac atatcacctg
     4441 atcaaagaaa cactccacag aggcccctcc tgacaatgag gggacccctg ccgtcaggta
     4501 gcatgaacca cattgcagca tagcaaagac gatttataaa attgaaaaag atcacgggct
     4561 ttattttatt ccaggatgtg ccgactcaat cctttttcca tatgggcaga catacaggca
     4621 caacccgcaa accgcgacgc cgcctgattg ttgcagttcc tcaaaatacc tgtcgcaggc
     4681 agcggcattg aaccgtgcct cccgggggtc gtccgggtcg aacattcgcc ccgtgaacga
     4741 gtgctgaggg cagatatcaa cgcatgcggt gcagtcgccg cagcgtggtt tcatcggcga
     4801 cccggaaggt ttgaggggag cgtcggtgag cacgctcacc caccggacac ggggaccgtg
     4861 attcggggtg acgagaaggc agctctttcc tatccacccc aggcctgcaa gatgtgcggc
     4921 gagcttgtgc gagaagaccg aacgtatctc tccatcgtca gtgcgcctgg aggcaggaac
     4981 ggggaatgca tggtagcctt ctcgttggag aacgcctgcc aggcggaccg cgatctggtc
     5041 gagtaactgg ttagtcacgt cgtagccggt gtgccggtag aggatctcac cctcgggatc
     5101 atgttcagcc aggaggttta caaggtcatc cgacagccgg attccaaccg ccacggcaat
     5161 gggataccgg acgatacgtt cgccgccctg cctggagatg aagtccctgg ccggaccaag
     5221 atccgcaacc ccgtataagt cggcccccat cgagaaagcg agagatctga ttgcggcatc
     5281 aagtggagga gacatatttt ggtacatgta tcaccaccga tcgtgtgcac ttgttgatct
     5341 cttttaatgc cttatcaatc ccggttaaat acactgcagt tcatatcctg ttccaggtaa
     5401 aaggagtgac atggaatgaa aacacaggat atcgtaagga gactctggga cgaggcagga
     5461 cgcgggaata tcgcggtgtg ggctgacggt acgatcacat tggtaccaaa ggattacaag
     5521 ggagagaccg gcgggaagaa accggttgca atcctcaagc ctatcgccct ggtaaacaag
     5581 tatgatttcc tggattttgc gcttgcggat gaagagctgc tcaccaccat cgaggagacc
     5641 atccgggctg gcggcggcac ggtcagccgg ggctgaccct gtatgtatac ggggatttaa
     5701 accctgcgat gagtatcctc cgtatgggaa tggctttccc gtaatggatg tcctaccaag
     5761 agcaaagaca taaacgtcgc acccgggaga gtatgtagtt gattcatcgt acatctctct
     5821 gatttgcgcg catgaatgca ccgggtgcaa ttcacaaccg ttatgtaacg gcgaaagaaa
     5881 cgggtaggaa ggcaggcatc cggttgaatc cgataatcct ttttatcctt gcgatatccc
     5941 ccattatact catttttgcg gggattgtat tcctgaagca gtcgggcacc ctgatggcag
     6001 cagttggatg gctcctcacg gttgccattg ccatcttctt tttcgatacc tctccagagg
     6061 ttgccgtgta tgcttcgcta ggggggatac tggcctcttt tgggatatcc ctgatggtac
     6121 tcttcacgat cctccaggtc accatgatgg atctcacagg ggcaatacaa cgcatctcgg
     6181 catctatcaa atcaatcgcc gcggaaaggt acgagcagat catgatcctg aacgtcgggt
     6241 tcggatcatt ccttgtctcg atcggggcga ccccggtgac aatgcttccc cccatcatga
     6301 tggcgcttgg gttctcaccc cttgcatccg tggcactccc gtgccttgga tatgatcctc
     6361 tcacctcgtt ctcactcctt gcagtcccta tcaccttgcc tgcggcaata ttcgggtttg
     6421 atgccgttgt catggggagc catgtcgcac tgttcctccc cgtgatctcc acaggtctct
     6481 cgatggggat gctctgggtc gcagacggct gggagggaat acggaaaggc tggatccccg
     6541 ccgtacttgc cggcctcagc ctcggaatct tttcgatcgt gttcgtcaac atgctccctg
     6601 catcggcaat agggctcgtc ggcgtgttct ccgggctttt gacgatcgcc ctcctcttcc
     6661 tgtaccgggg ggtccgggga aggcggttga tagcccggaa agaagatgca ccgggacagg
     6721 tggtctcacc ttcagcatcc gccatgatgc cgctctggaa ggcagtcctc ccttgggtgc
     6781 ttctcgttgt tctggtggtt atcatcagca tcgaggacat ccagtccggc ctctaccacc
     6841 tccttgggga agcccaggtc atccatgtcg tgggaaacag ggccgtcgat ttacgactca
     6901 tgaaccaggc ctatttctgg gtcgtggtga gcacgctcgt tgcggcaccc ttccttgtcc
     6961 gatcccggaa tgaaggaaaa aagatcatca ggctctggct gaaacgtgca tggagtccca
     7021 cgcttgccgc ggcagtcttc tttgcaatcg cgtacgtgat ggactggtct ggccagtgta
     7081 tcatcgacgg caccctcacg ttcgttgcgg gttcgaccga cctgaacatg aacgccgtga
     7141 tcgggcttac cctggcgatg gcgttcggga ttgccttccc aatcatcagc ccgctgcttg
     7201 gattgctcgg atctttcgtt tccgggagcg agacctcttc gaacgtgatg ttctatggga
     7261 tcctgcagaa ggcaacgaac gtactatcgc tcgactttga gaaagtctat gccgcccaca
     7321 tggtggccgg tggcatcgca tccgggatcg ccccggccaa gatcatcaat gccgcagcag
     7381 tgattgacca ggcctcgatc gagggagaag tgatcaagaa atcggccgtc gtggttattc
     7441 tcctcgcatt gctcacatct gcactcctgg tcctttcact tgtcatctga agggattgag
     7501 aatcccggcc gccgtcccat gcgaaggccg ataagagaca ggcaggatgg aagggattgt
     7561 tccctttgcc atcctcaccc gatgactggg aggccgggac agttgcagtc cctcacgaga
     7621 ccggcgtcca tccctcttct gaaccacgat acccgctgtg cggaagtacc gtgggtgaaa
     7681 ctgtccggca cgacgtatcc ctggtattgc atctggagag tgtcatcacc gaccgcactc
     7741 gccgcgttca tggcttcttc gagatcgcct gattcaagga tcaggtcatc gtacattgcc
     7801 tgcttggccc acacacccgc atagcagtcg gcctgaagct cgagcgccac agagagctcg
     7861 ttcgcatcag cgtctgagag atagcgtgtt ttcctgtcca cgtacccgat ggtcccaaga
     7921 agattctgga cgtggtggcc aatctcatgg gatatcacat atgcctgggc aaaatcgccg
     7981 ggggcatggt attcctgttg cagcgcatag aagaaatcaa ggtcgaggta caccttctca
     8041 tcggcggggc agtagaatgg ccccaccgct gcgctcgcct gtccgcatgc tgaatctaca
     8101 tatcccgtgt agaggacaag ttttggttcc ctgtaagttt tcccaagctg gtcctcgaag
     8161 atggtggccc atgtactctc cgtatcgccg agcacgagtg ccacgtgatc agcaagttcg
     8221 tcctgttgtg tgtctgtgag cggggactgg tatccatacg agtccgaagc gtaatcagtt
     8281 ccgctgatga tctggccagg gtcgacaccc atggccactg caaggacaag aaccaggatg
     8341 agcgcgagcc ccccgattcc tgtcgctttg ccccctggaa tccccgagga tcttcccata
     8401 ggaatccccg aggatctccg gtcctcaata ttggtgctcc gtcgttccgt ccgccacctc
     8461 atatctctca cattccttat ctagtccctg ccatgaatcc ggaaccttcc tttgcgttcc
     8521 cctcataccc gattgtcctg tgttgattga taaaatggtt cctggaatcc atcgcaggtg
     8581 atcctgcact tcccgattgt gagggattgg gaaaaatatt ttccggaatg ggcagggcga
     8641 cataggtcat ggtaaccctc atccatcgcc atacctcatg tgagagcgta atccttgaga
     8701 tatagttggt gaaggtgctg aaatgtgacg cgccttctca atggagagta aagaaaatcg
     8761 gctgtcgtga ccacggtgcg gcggatggga cgttcctcaa tcgttgaaat gcgcatagag
     8821 ccggtgggca atgagctgga acatcacgaa gaaaatcccg agcatcgcca cgtcaacgag
     8881 agggtcaaag gatgccggat gcccgtaagc gcattggatg aggtcgttgc cataggtaag
     8941 gggggagaag tatgcaattg ccgtcccgat aggtggcatg gcatcaaggg gaataaatgt
     9001 ccccgaaacg aagatcagcg ggagccggac gaggttcagc atcgacatga tgtctcccgg
     9061 gctctcggtc ggataggcgg caaagagagt tcccatggtg gagaagcaga gtgcggaaag
     9121 gagcattccc accgtgagtg caaggggcga ggatatggtg ttcccgaacg cggcaacccc
     9181 cacgacaaag gggacgagcg cgatgccgat gctgtacagg aacccggaga gactctcgcc
     9241 gacgacgata gcgtgaagag agaccggtgc cgagaggaga cgttcgaagg tctttgtcct
     9301 ccgctcgatc gggatagaaa ccggctcgat ggtggaagag gagaacagta tggtcaccga
     9361 gatgaggcct gggatcaggg cgccgagcgg gacgttcttt ccggccgcaa acgcaaggaa
     9421 catgaagagg ggaaagagaa gcccggcgat gatgatgttt ggcttcaggt agtaaatgat
     9481 catgtccttc tttgcgatag cccaggcagc aaccgcctcc gcagcgagct ggttcctgtc
     9541 aaagtccgca atcacggctc ttctccccca ttcttcgggc gccgaagccc cgtcagccgt
     9601 atgaagacat cctcgagtgt tggtccgagt gtcgtggcgc tcactacccg gtcctggtgc
     9661 gaatccgcgt aggtgacgat ctcccggaga agcccacccg gatcttccgt gtagagtcga
     9721 accttgtccc cgaccttcct cgcctcgctc accatcggga ggcctgcaag gtcagagagc
     9781 acccgggcgc ccccccgctc aaatgccact tcgaccgact ggacgctctg gatggtcttt
     9841 ttcaatcgct cgggcgaatc gatcgccgcg atccggcccc tgttgatgat tgcgacccgg
     9901 tcgcatgtct ggttcgcctc ctcgatattg tgagtggtca aaaagacagt aaccccccgt
     9961 tccgtcaggg agtggagcac gtcgcggatg atcaggttgc tctggacatc gagcccggag
    10021 gtcggttcgt cgagaaagag aagcttgggg tcattgatga ggcccattgc gagggtgagg
    10081 cgccgcttca tccccttcga gaaccccttg accctgctcg cgcgacggtc ccagaggcca
    10141 aacacctcga ggagttccct tccacggctc tcgcggtcat cttttcccac atgatagagc
    10201 tcccctgcaa acatcatgtt ctgccacgcg gtgatgtcgt cgtagacgtt cgaggtctcg
    10261 tacacgatcc ccatgtccct cctcgccttg atcgtgtccc tgacaatgtc ccatccaaag
    10321 atggtggcag aacctcccgt aggagcggtg atgcctgtca ggattcggat cgtggttgtc
    10381 ttgccggcac cgttcgggcc gagatagccg aacacctctc cggtctcaac ggcgaaactt
    10441 acacggtcga gtgcgaggat ctcgccgaac cgcttctcga gacctttcac ctcaatggca
    10501 tgcatggaat gggcgcgaca cctgcccttc accgtgtcag gtatttaaag agagacaaga
    10561 taagggttta gaaaaagtcc cgggacagaa gcgtttctca tcctggctcg cagcattgcg
    10621 aaaaattcct gcggcctcac ctgccaggta tccggaggaa aataataatc ccggccttca
    10681 ggatttgcgc gcaaggtacg cacgcaaaaa gaaacgatcc gatctggcgg tatcaatcca
    10741 gactgaaaat tcgcgggttt caaaaaggcg gaaaccggat tcttccagcc agggacgaac
    10801 ttcccggggc gaatgccgga acatcctgac aggcgccgtt gttctggaat gttcgggagg
    10861 gatgactgct gcagcgtcct ccccctggcg ccggtcgccg gtcacgaaca caaaaatccc
    10921 gccgctcttc agaatgcggt tcacttcctg gaatatcagc gagaggtttt cgaagaactg
    10981 gaataccccc gttgagaccg catggtcgaa ggaatcgttc tcgaaaggat acgggaatac
    11041 ggtgagatca tgacggacaa ggcgttctac gcaacccttt gcacggcatg catcgagcat
    11101 ctcctcggag atgtccaggc cgaccacgcg taagccggcg caatggaata attcggaacc
    11161 aagcccggtg ccgatcccga tatcgattat ctcctggcct ggacgggtat aaggtgatgc
    11221 aagcgcatac acaattgcag gggcatccca gccgatcgcg gttgcctctt tatcgtagtc
    11281 atttgcaacc ttccaactgt tctctctcaa tttttattcc tccccctggt atatcgcagg
    11341 atggtgggtg tattcatccc catccaatgg tgattgggca gcacgtgcat aattctagta
    11401 accgttattt gctcaacaat cttgataggt tcaaatcagg ggaatacact attgatgctt
    11461 tctttttagt gcacaaacag gaggcaggga gacgccggac tatggagaag ccatgattct
    11521 tgctatttcg ggcgatgaaa aacatactgg aggagcaggt aagcgattac aaaccacatc
    11581 agggataatc cccagatgaa cgcaagcgcc tggaatggac cagtggagcc gggatcaaac
    11641 gtcaggatgg tgaggctgat tcctaccagg accgcgatac ataacgcata ccaaaaattg
    11701 tcgttgagga gagaacctga tgcagcggtg cgaacccggg tggcactgcc taccgggtcg
    11761 agagggttgc ccgatctcat cctcagctcc ttcactttct cattgcaccg tctcgcctca
    11821 tcgacccttc ccagtttcag aaatgagagc ccgatattgt tccatgcctc gatgaagtca
    11881 gggttcagct ggattgccct tgcatagaac ctgagtgcaa tcccatagag tcctttcttg
    11941 aagaacttgt ttcccgagct cttccagacc acggggtcgt cgactgcgtt accccggtaa
    12001 tgactgcccg ccatgatgcc aagtattgca tatacgataa agggaaataa atatgtctcc
    12061 tgttcaaggg atggaccctg gtgaggaaaa ggccggacat gatctggaag cgacccgcct
    12121 ttgccccatt cgggatcccc cggataaaaa acgctcacgg tcaaaaggaa ataatttttg
    12181 gatggaggga tgggtgctct ctaaagatac attggaacaa accaaaagac cacttatggt
    12241 ttccttcgtg catatccctg ttctgccagg gatttaactg ctgcattcgc ctcttcgaac
    12301 ttctcctttg tctggtcgat tgtgcgagag agaaatggaa ccagtacccc ggagaatttt
    12361 tcatgctgga taagccggga acactcactg gattcctcga ttatctcaaa actgtgttca
    12421 ccatcaaaga atcccggaat ccagaggcat cctttccaac ggatgtaccg gaatggggat
    12481 gcggcgagaa gcgtcaccgt cagggccatg gtccttcctc cgctgctcat catgaccctg
    12541 aaaggctgtc caatcctcat ttcgtgaaca gcatccccaa tctccggagg aatagcattc
    12601 gatgcgagaa gggctttcca taccacatca tcaggtgcgc tgatccggat ctcggcacga
    12661 atggttcgca tggacaggtc tctggaacag gagtctgact acaaaggttt aaaaggctgc
    12721 catatcattt ctgtcggtca gccctcacaa tttcagccat actgagcctt ctcgatcgcg
    12781 cgcttcagct tgtcaggctg atcagtcccg atccggaact tctttcccga gacaagtgtc
    12841 acctccactg cctcgaaccc tgcaatatta tagagcgggc cgtaaggtgt ccagcggatg
    12901 ccccacccat agtaccacgg gttggtgacc gttgtcactg aggcaatctc acagattggc
    12961 cagatcttct tgaaagctct tatgggtcca aagcatatcc gcagtgcatc ctttcgtacc
    13021 gacaccgtca gcgtggagaa gatcgcgagc ataaggacca tgatcccgcc tacaatcacc
    13081 gtaacccaga cagggccgaa gacgactatg gatacggcga tgaccaggag cacagccagg
    13141 actgctgcga tgatgagggt tccccgctgg gtgtgctcgt actcccattc catgggtttt
    13201 cctgaacggt cgaatcctgc aggttcctgg ttattcaggt tcacttcatc aatcccttgt
    13261 aagtatgatg gcgctttcgg gacagacgtg gaaacataat gagcagccgt cgcaccgggc
    13321 attatcgaat tcgggtttcc cgtcatggag ggcgatcgca tggtagcccc cgtccctgca
    13381 cgcgataacg cagcgaccgc aggacgtgca ggcatcaggc gaagagagta ctggccgtat
    13441 catgcgtgtc ctgctgagtg actggtggga ctgaagtctc ccgagcgcaa ggcccctgag
    13501 atggtcgggg gaagagaatc cctttccggc aagttacgat gaaaggcctg actgcatctc
    13561 ttttacgatc ccaaagccgt gccacatgac cgcggtgcag acctggaccg aggaggcgcc
    13621 ggccagcaga tactctgcgg cgtcgcgcca cccggatatg ccgccgatac caatcaccgg
    13681 caggtctacg gcagatgcga cctgcgagac gacccgcaat cctatgggtt tgaccgccgg
    13741 gcccgagtaa ccgccatagg tcgagtaccc ggcaacagag gggagaggct cgaaggagtc
    13801 gaggtcgatc cccgagaggc actggactgt gttgatcgcc gagatcatgt cagcgccgcc
    13861 ggcagaagca gcatctgcga ccaggaggac atcagtcaca tttggtgtca gcttcacgat
    13921 gaccgggatg tcggctgcgt ccttgaccgc acgggtcagg ttccggacca tcgcagggtt
    13981 ctgcccgata gccgcaccca cgccctgctc aggcattcca tgcgggcatg aaacgttcat
    14041 ctcgatcgca tcggctcccg cttcttcgac ctggccggca aggtttctcc attcctccgg
    14101 gtcggtggtc cccatgatgc tcgcaaccag gaggcgatcc gggaactcct gcttgagcct
    14161 cggtatctgt gaagtccagt aggatacggt cttcttgctg agcagctcga tattctcgaa
    14221 tccctggact tctgcatcgc ttccttttat cgcgtgaaat ctcggcgaga catcctcgat
    14281 gaccattccg tccgggcgga tggtcttgat cacggccccg gcccaaccaa ccgagaatgc
    14341 cctccttatc tgttcatacg atgcagtggg gggagcggac gagagcagga acgggttttc
    14401 caggtgaaac ggtccaagcg tggtggcgag gggatgatct gcagtcattt cttttctact
    14461 caccatgggg aatgggaggt gtttgttgtt gtgatcgatt atctgacctg gactttgtga
    14521 tcgtgtggtc accatccttg tctgttcctc gacggtatcc cggtcgccga tgaccttccg
    14581 gcgatcaatc ctggcgaagg atgcattctt gtatcattgg atcgtccttc gtctcgccca
    14641 ttccctgccg tatacatatt cattcctaac cgccggctca agggccagcc agaacaggac
    14701 gaggcccaca gtaagtgccc acaggatgtt gtttccaaag taactgacaa acttgctctc
    14761 aaaggggcgc ccgaagaaga ttgtcccgca cgcggcaagg aggagcatgc aggcaagaat
    14821 gaccacgaac gagaccagca ggaaaatgcc cgtactctcc ttgtccaccc gtatctcatg
    14881 gcatctcatc agcctgtgcc agagcagata cgagagatac ggggagagga aattcacaag
    14941 aaacccgaag atgctcatcg gggaccagga accatagaaa tcaccgggga tgttcccgat
    15001 ggcaaggccc catgcggctg caggaccaaa gaggatgcca aagagtaccg ggagcgctgc
    15061 agccggccgg atggatatcc ctgccacgtg aaggtcgagc tggttgaagg ggattaggat
    15121 catggcataa agaaatgctg ttgctgcgat ccaggcgagc cccctccgtt cggtccatac
    15181 cgcggcaagc tcatgcatac caattcgtta ctccgccggt gaaataaaaa agccggatat
    15241 gagcacaggg ggtcagcgtt ctgtatcaag tgggatgacc taaatcaggg cgaccgatcc
    15301 cgggaattac agcctgaagc cggtgcagta tcgaacagtg tctgatacaa cccggcaaag
    15361 agggcggggt cttcaccggc aggcgcagtg gcaacccggg tggtcttctc cccgtcaatt
    15421 ctccaggaga tatacccctc tcagtaagaa aaccgatata gtcctgcccg gtcttcgagt
    15481 tgagggtgca gcgatggaaa atcgaagtga atgatcttgg gggtttgcaa aagacgagga
    15541 tctccctgta gatctggata tggcatgcgg cgctcggcgt tcatcgggag tatcctgcca
    15601 gcgtatccgc gagtatctgt tctgcccggg aaatctcacc ttcccggaca tcccatgcgt
    15661 tcgaaaacct ccaaaagtag agggaactca acaagctgac gaatgcaatg ccaagagcgg
    15721 ggagggctgg ggggatcagg cgtgcattgc ccagggtgat tgtgccagaa gtaaggcaga
    15781 acgagaaaaa ttcgatgctg ctgaagattt caagagcaag gaagcagaac cccccaagcg
    15841 tgcagatgca gatcatggtc cggatggtct tcatgtcctc aaggtaatgg gcaatcatcc
    15901 tgatgatccc gcatatcgtg acttctccag ggactggggc tttccgatct ctggatgcac
    15961 tccggatatc gtcaaggcca tccatgatct tcactcctga tatgacccaa aagaggccga
    16021 ggccacatcc tgccatggag aatgcggcgg atgttattcg ccacaatgag aatggatctc
    16081 cctctgcaag gccaagcacc gaggataaaa aatactgcac tccgaatgcg atcgccagtg
    16141 cgctaaagac cacgttcatg aggatgatca gagagaaaat attgagtttg taaaaaatcg
    16201 tgaattcacc gtgtcagtca tgtgtgttcc ccatcaacag ttatacctgc gatttcactt
    16261 gaatctccat gaacccgtga tacatacctg gaatcttgct tcacaggcga acgatcgatt
    16321 tcacacctcg attgccaggc catcagttgc agcaacgagg cccgggcact cttcttcgta
    16381 catcctctca atctcgaacc tctcttcaag ggtggtgtag gtgtgggcgg caaaatgaac
    16441 cagggcaagc cgttttgcac gtgcttcccg tgcaatccgg atggcatccc cggggttcag
    16501 gtggggccat tccgggctcg tttctcctgg tttaagtccg cattcggtga tgaggaggtc
    16561 cgcatcgcgt gcaagtaaga cggcattcgc gcagaagccc gtgtctgtgc aatacgcgat
    16621 cttctttcca tcgagagtaa gtgcgtaccc aacacacggg gcagtgtgga caagctgcct
    16681 gcactcgatc gaaaatggga attcatgggt cccttcgaca agctcgatga acctgacagg
    16741 aaaaggcaga tgatcgaacg gtacagtgaa cggttcccgc acaatcgagt gaagctcccg
    16801 ggagatacct ttctggccgc agatgtgcag gggaagactg aacctgaatt tattgagagt
    16861 gtgcagcccg gcgatatggt cgaggtggca atggctgata agaaggaacg tgggcaattc
    16921 ctggtcgaga tactggtcag cctttgcgat gccgttgcca gcatctagga tcacgttata
    16981 gtcttttgac tggaccagta ccgagcaggt attcccggtg agggtgtcat accacccgtt
    17041 tgtcccaagg aatgtaacca acattctcca gagtggttgg cgcggaaggt gaaaaaccgg
    17101 gcactctcaa cttttgaaaa aaccctggga atgaacggga tattatgtcg agaaaggaaa
    17161 tggggtcccg tcttgtccaa ggcccggatg aagggaacag gatgtgcgta atgcagggag
    17221 catcgcagga catccctggc ccggagcgaa ccaatccgcc ggaaaaatga tatttatggg
    17281 taagatcagg ccggcctcgc ctggatctcg gcgggaagaa tccgttcgat gaggaaggag
    17341 gggacctggc gcgaggtgcg gacgatcagg aaaaagtccg cattcgggcc tgaacggccc
    17401 cggtacgttg cctctatcag gtagtttgtg gagaggaggg ggattccgag cgccctgaac
    17461 tctgccctcc gttcggagat ggtctttgtc tgcccggcaa cgaacttctc gttctggtcc
    17521 ccaatcttca tgatcaactc gtcgcccgaa gtaagaacaa ggatcggagt ccccgcaatt
    17581 ctgatcggca gcccatcagg aatcaggact gcatacgtcg tgatataggg atagacggtg
    17641 ccaggttccg gcgggaggtc tgcctggacg gaaagggtaa gaaatgcggc gatgatcagg
    17701 atgacgagga tgacgattac cgctcccgcg atcagggaga acctgggaat cttcttttca
    17761 gttgggggag caggaggcct ctcatccttc cctccccctg aatgtggttc atcggccatt
    17821 gtactcatca ggtagagtcc agattatctc acttatatct tgcgtaatcc agcatgcttc
    17881 atcagggaag atgccggggg cgtcattctt ggttgcctgg ctctcagatt gcccgcggaa
    17941 cagggaaaga cgatcgcact accttcaatt ctccatgtgc agcgaatccg ccacgtcccg
    18001 aaggccgagg cgttcgagtt cggtccggtt cgggatgccg gtcagagggg tgcagccatg
    18061 gagggagtaa taggcatcca ggagtacctg gtgccgtgcc aggtcttctt ttcccttgct
    18121 ggtcccggca acctccgggc gctgaggcat cctgtcatct gcgcgggtca tcccctgccg
    18181 cacgttgaac gacatctcct ggaggtagat acggtcgagg gcatcctcca gttccctggg
    18241 ggtgtactca attcctgttg cggcccggat gaggcggatc gcccctcccc agagaggaaa
    18301 cttcctgacc agcggcaccg atgagttcca gctgttcacc gcccccttgc acctgcccag
    18361 cgcatcggca agcgtagtgg ctcgctctcc caccctgagc gcacctgccg gatcggttgg
    18421 gatgtatcct gattgcacga ggtcccggta gagcccctcc tcgttctcgc ctcctgccca
    18481 ggtcctgccc ctgagatgat ccgctcccct ggtcgaggtt gcatgggcaa ggctgtaaat
    18541 cccagaggga tacacgcccc gggttacccc cttgacgtca aagcaatacc gggctgcgtc
    18601 ttttccgagt ctctctgcaa gtccggccat gccgagcgcg agaaggtccc caaaaccctt
    18661 ccgctccgcg gtcatggtga tgagaagtct ctgcgactct gaatcctccc agtcaaggga
    18721 aatcccaccg gtatcctcca gggaaatgat gcccttctca aagagttcct ttgcaaaacc
    18781 aaccacgctc ccaaggccga tcacgtccat gccctgccgg tcggcgtagt tcgacatctc
    18841 catgatggca atggggtcca gtatcccgct gttgatcccc agccagtgga tggcctcgta
    18901 ctcgagccct tcccctacct cgcccttccg gggaccttcc gggatacgga tgatgttctt
    18961 gcaccggagc gggcagccat ggcagccggt atggccgact tcatactcct tccatgcatc
    19021 cttccgcagc gccggtggga cgtccgcacc cgtccatcgc ctctggctgt tccgaaagcc
    19081 cggcccccag tcctctaccc cgatgatgga accatatttg ctgacagtgt tgcggaagaa
    19141 cgggtcgttt ctgaatattt cccgatcaat ctttgcgagg tcacggaacc cttcgggatc
    19201 agcaagtctg ggagggttcg aacccctcac tgcgatggct ttcaggtttt ttgaaccggc
    19261 gaccgccccg ctcccgattg ccgccgaggc atacttgtcg atgatcgtcg aagcaaagcg
    19321 gaccagccgc tctcccgccg ggccaatgca ggcgacgctc gtgtcatcgc cgagatcttg
    19381 ccggagacga tccgtcgctt cccacgtatc cagtccccat agtgaccggg catcacgtat
    19441 ctccaccctt tcgtcatcga tggagaggac gcaggggtaa ggggatcgtc ccttcaccac
    19501 gatctggtcg tagccagcac gcttgagctg ccaggcaaat tccccgcccc cgctcccgtc
    19561 gcctagtatc ccgccaagcg gcgagagcgt gctgaccgag accctccccg tcagcgcaag
    19621 aggggtcccg gtgagcattc ccggtgcgta acagaggacg ttccctgggc cgaggggatc
    19681 tgtcccggga gctacctcct cccagagcac tctcgaatta aaaccccttc cgcccaggaa
    19741 tttccgcgcc ctgccagcat caagggcgcg ccgctcgatt gaggaggtgg tgagatctat
    19801 ccggagtgtg gcccctgccc agccgtacat ggagaatcat ctctcttcga agaggataaa
    19861 tcacaccatc aagacgaagg agcccgctga gactccagat ccccagcgtt ctcttgttaa
    19921 aggtgcctgt acatggccag gcattccggg cagccggtcc ccgcgcaggt atcgggatca
    19981 gagagggagt cgagtatctt cccgatcaca tccgggtttc tcggcaggtg caggtggcaa
    20041 tagagatcgg gggagcgggc gagagaggag ggatcgaggg ggaggatgtc gtattctgcc
    20101 ccctggagcc aggagtctgc gtgggggact atcccatctc ctgcataggt cgtctcccag
    20161 ccattcgcgg tcatgaccca ggttttcccg tggaaaggag ggaagaactc cgggtcagcg
    20221 tcgcagttcc cggtgaggat gaaccggtat ctgatatctt cgcggatccc cgcagaacgg
    20281 agtgccgcca tcgcttcgct cccgggcctg aactgctgga cgatgatgtc gtcagcagga
    20341 ttgaaccttc tcggcacgaa gatcccgcca agggtcttca gaatgcgcgg cgagtgaact
    20401 ggatcaaaga agagctccgc gaggcaagac ccgttgttgg gtggcccgag tccaacgagg
    20461 agccgcacct tctcacacct atcgttttta tcaaggactt caatgaggta ccgggcgatc
    20521 cctgtcccca tggagtggca gaccatgtcg atcggtccgc agtaccccgt atcagccctc
    20581 tggtctcgaa tgaactcctg caggagagcg gcaagcgatt cgactgcagc tccgcgaacc
    20641 ctggagtgat cgaacgtcca ggaagggatt gcgtccttat ccagtgcttc cacgagccgg
    20701 tcccagatac cggggtggct cttccaccca tggaccagga caacaggcct gccggttccc
    20761 gtcacgaaca gaatatgtcg tctgcttcac cgatagaggt tgtgaatcgg gcaggcggcc
    20821 cccgcccgaa cgcgggccga ttgccccatc cgctaccagt ccttcttcat cgcctccgct
    20881 ttcaactcct ttggcatcag ctcgattgca tagcggagcg ctgtcctggg catgttcctc
    20941 tttctttcca tgacgaagga gaagacctcc cccgtgtgca tccggctcgc ctctttcagg
    21001 agccagc
//
LOCUS       DAOQYR010000031        20438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_2986_length_20438_cov_8.780994, whole genome shotgun sequence.
ACCESSION   DAOQYR010000031 DAOQYR010000000
VERSION     DAOQYR010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 20438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20438
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2986_length_20438_cov_8.780994"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..3480)
                     /locus_tag="PKM87_05395"
     CDS             complement(232..3480)
                     /locus_tag="PKM87_05395"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ29539.1"
                     /translation="MKPGNQIGITGKGAGYAALIIAGILLVCTVASADEVLPLPDFTR
                     ISEGGFGDPQNNYAWAVAEFNGDLYVGTGRNIPYFVAQAMKARGAFPANWTLSFLTTP
                     SGSPPPPLVLPNHTPPSQEDVIEWSNDMRAEIWRYSGDSWERVHQATTFINPANGYTY
                     PEGIGYRAMTTFSDVNGTEGIYAGVGFGFGPILVVGSTDGDTWVPIRTSSIPSRDTRA
                     MISHNGRLYMGTGEGIYATGSPSPHEDNWVKVADFQTSSLESYNGYLYAGTGNPIGPS
                     ETNGFEVWRSTTASPEGPDDWVKVVSGGAGDAWNVLAATMREYDGDLYVGSMNLPFGT
                     GTEGVKGFDLIRLDSDDSWDLIVGNIHPRIPTYPRGLPMSGWPSGYANPFNLYAWSME
                     EYHDDLYLGSFDIFSFGRFIEEVPGGTDILFEALESLERGEMSGEFSEELEVLRDMDL
                     EHMEEVDLALIIKLLAIHFGGADLWRSQDGVHWVPMNLNGLGDPDNYGLRTMRATDDG
                     MIIGTANPFAGCQVWVATTPDPEPPVADFFAIPRSGDEPLSVIFADQSTGGHLSYYWD
                     FGDGTYSTKKENVHTYVSAGTYNVTLTVTNSAGSSTECREKYIKVTKDEPLKANFTSN
                     LTIGAVPVTVQFYDTSTGSPSSWSWVFQKDSYYLLRDAATKQAIPYLGNEFSAEKDPV
                     VIYTSPGNYSVSLTVSRTGETDTVTKEGYIQVDPPAPEADFNAYPMEGDAPLEVEFWE
                     MVPYSWCYDEFLWDFGDGTTGSGSWLYHTFDAPGLYNVTLTVTSAYGNSTLTKEGFIN
                     VTQSLPPVPDFEASPLSGSAPLGIAFTDTSTGLVTSRLWDFGDGTNEWRNESTDVDHT
                     YPFPGPYTVSLTVGNAAGQETVTKPGYIQVSSSGAPPMAFFTMRPMSGIAPLTVAFTD
                     RSMGTPLAWEWNFGDGTTSTEQNPVHTYTGTGTYTVTLRVFNHGGSNSHSSYVFVRSA
                     STVFLPVTTSSSGTAAPTATATPAPPLMPGRAPISFFMVSKSIGFAPMTVAFTDMSFN
                     GPTSWDWNFGDGQTSTIRSPSHTFIIPGTYSVSLKVANTHGESTTSRRIYVR"
     gene            complement(4059..4265)
                     /locus_tag="PKM87_05400"
     CDS             complement(4059..4265)
                     /locus_tag="PKM87_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFYB/HAP3 family transcription factor subunit"
                     /protein_id="HNQ29540.1"
                     /translation="MADLPIAAVVRIAKQSGAERVGSDAAAALVAKAEDYIAELVKEA
                     NKLAMHAGRKTIKEEDVELAAKSV"
     gene            4763..5053
                     /locus_tag="PKM87_05405"
     CDS             4763..5053
                     /locus_tag="PKM87_05405"
                     /inference="COORDINATES: protein motif:HMM:NF020317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HNQ29541.1"
                     /translation="MPCFIILGKLSDRAIERMKDAKDRDAETKKIIEDAGGTLIAHYY
                     TIGRYDVVAIVNLPSEEVLAKVLIGIGKCGTVSTETMTALLPEQMYRMAKGT"
     gene            complement(5274..5765)
                     /locus_tag="PKM87_05410"
     CDS             complement(5274..5765)
                     /locus_tag="PKM87_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29542.1"
                     /translation="MNMINFTRLTGIVMVVLAIFTCSAGASFVSGNFESPFDTYPFSG
                     YQFDGYPFDGYPYDGYTPGGNPFDGYPFYESPFDTYPFSGHQFDGYPFDGYPYDGYTP
                     RGYPFDGYPFHESPFDTYPFSGYQFDGYPFDGYPYDGYTPGGYPFDGYPFHESPFDSY
                     PFV"
     gene            complement(6008..7483)
                     /locus_tag="PKM87_05415"
     CDS             complement(6008..7483)
                     /locus_tag="PKM87_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HNQ29543.1"
                     /translation="MNPQADIPQEETLDPGDWDSMRALGHRIVDDAMDYLETLRERPV
                     WQHAPPHVKAHFEGPPPAGPMPPGEVYQEYLRYILPHQLGNAHPRYWGWVTGSGTVIG
                     MYAEMLAAATDAVSGAFSYMSNNYVERQVLDWCKDLLGYPRTASGLLTSGCSASNLIG
                     LAVARNTLAGFDVRGKGMTDQTRRMTLYCSEEAHSSIDKAVELLGFGSESLRRVSIDE
                     LLRIDVAALEEAIQRDRKSGCHPICIIGVAGTTNTGAVDDMNALADIAAKERCWFHVD
                     GAFGAWAAIAPRSKHLVAGIGRADSLAFDLHKWMYLAYPIGCILVRNADDHRRAFSLT
                     PSYLAHGEGDRGLTGIDVPWLSDYGFELSRGFHALKAWMTFKEQGTAKYGRLIQQNID
                     QAHYLARQAESAPELELALPVSLNVVNFRFTVAGVPDGRLNEINKQIEIEIQEQGIAV
                     PSTVTIRGRIFLHVAITNHRSRNDDFDLLVREVIRIGKELT"
     gene            complement(7731..8186)
                     /locus_tag="PKM87_05420"
     CDS             complement(7731..8186)
                     /locus_tag="PKM87_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29544.1"
                     /translation="MKLTTTCILACLACILVLAAGCTSAPAPAQTPTPTATTVPATPV
                     PIATTASFSGTWNTKWYDEEGEHPDLMFLEQAGSAVIGMYSESNGTINGTFGGSRLVG
                     TWVDYYENETVTGVFEFTLSPDGNSFTGKWADTLEELANATEFWNGVRV"
     gene            8549..9019
                     /locus_tag="PKM87_05425"
     CDS             8549..9019
                     /locus_tag="PKM87_05425"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNQ29545.1"
                     /translation="MEDITIVEVPSQQVVGIRKSGNYTLIPELLMQLAEYIMENNIAF
                     AGMPMFICHETSPDAVKEANEKGTALVEVAWPVAGKVSGSGDIKVYTLPGGKMVRTVH
                     KGPYDTCEPTYLALFSWMEKHRLQVAGPIREIYPNDPREVKPEDILTEILVPIR"
     gene            9448..9795
                     /locus_tag="PKM87_05430"
     CDS             9448..9795
                     /locus_tag="PKM87_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNQ29546.1"
                     /translation="MKKSFHESFREIVFWMSDGTVSIFGLFAGMVAGTQSSDVILLAG
                     ATAAIAATVSIMAGVFLDLESDKDEARVEAEERAAEILHNPDRAVAKLMNDPERSDLS
                     KGKPRCNPCRPPE"
     gene            9806..10120
                     /locus_tag="PKM87_05435"
     CDS             9806..10120
                     /locus_tag="PKM87_05435"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNQ29547.1"
                     /translation="MQKFEDAVASEEVPAGKKASPCVHACWMGIADFVAAMTPVIPFA
                     FLPPEMTKIACVAGTATLLFLRGIARARPGKRPVVRTVLETMAIATAPGVAGLGVGLL
                     IT"
     gene            complement(10252..10773)
                     /locus_tag="PKM87_05440"
     CDS             complement(10252..10773)
                     /locus_tag="PKM87_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29548.1"
                     /translation="MNSRDERGRTYEKVSADRTREELIYAVLSLPVECDPRVRTLVET
                     LNDLPDIYTCSSCGGHHDNVGRENPAPEGCFYLQFIVEPTEAGFLSLGIIDLAARNVD
                     CERLAVQVLNTTDSPRLVMFHMLGREGADPDAVAEEVRSLCRSWKVSLRGVREHKENV
                     RKKKALEKITSSF"
     gene            11138..14050
                     /locus_tag="PKM87_05445"
     CDS             11138..14050
                     /locus_tag="PKM87_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ29549.1"
                     /translation="MEDSIRVLCVDDEPSLLALARMFLEKSGGFFVGTSTSAEEALDR
                     HEIMSYDAIVSDYQMPGMDGIEFLKAVRLRFGDIPFILFTGRGREEVVIAAINNGADF
                     YLQKGGDPKAQFAELAHKVRQAVRRKKAEVSLKESEEKYRMLVEHNRDGVFIVQDGKL
                     VFYNNALTGLSRYTPEELEGMHLADLIAPEDREMVLSRAKVRAEGGLVPERYEFTLVH
                     KDGIHRSRMRISTYLGSYQGRPASFGTFYDVTEDRRREAALRESEEKYRTILENIQDV
                     YYRTDTHGNLIMLSRSGYRLLGYDRSENILGSSLAGTFYANPEDRRKLLEAISEKGAV
                     HDYEVNFRHKDGSIITVSANSHYYYGNNGKIAGIEGIFRDITEQKRAGEDLKRSENLY
                     RTVFEHTGTATILIGPDTTILKANGEWERLTGVPLAAQENILSWTGFIHQDDRERMKE
                     YHYARRKDPAAAPGIYECRLVDAHGGIHYGFVHVGMIPGTGNSIASLVDVTALTQAQE
                     DLKASERKIREILDHLPDLIVVHREGNILYVNPAMTESMGWQPDEVVGQPILKFIAPE
                     FHAAVQDAARLRLESGIDSPYIINLINRDKKPLTVRVRGTVISVSGSPAIMNILTDIT
                     TVRNAEKALEESERKYRMLVENTNDIIYTTDLAGQITTISPQIRRYGYSPEEIVSENL
                     TSFIVEEDLPRVIADFEKTVSTGQSTLTIFRVRDRSGTIHWFEDNGSALTDASGNVIG
                     ISGILRDITDRRTADDALVRANRQLNLLGGITRHDILNKVSVILGYLEIAKESVKDPE
                     LGGYLEKVESATRAIREQIEFMRLFRDIGTRAPEWISLDSALPHRHVPATISFHAEVI
                     GVDILAEPMVERVFYNLLDNSVRHGGRVSAIRVSAYRSGDSLVIVWEDNGVGIASGEK
                     ERIFDRGVGKNTGLGLFLAREILSLTGITIRETGYPGTGARFEITVPRGNFRMAAE"
     gene            14094..14585
                     /locus_tag="PKM87_05450"
     CDS             14094..14585
                     /locus_tag="PKM87_05450"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNQ29550.1"
                     /translation="MNAIILIGMPGAGKSTVGVILAKALGLKFIDTDIMIQERTGKLL
                     QEILDWEGPRGFERIEEETILSLHPSHAVIATGGSVVCSRAAMAHLKSMGVVVYLKIS
                     YTEMERRIRNITTRGIVLLPGQSLQAMYDQRAPLYQKYADLTIECLDKDFETVVGKVI
                     AGL"
     gene            complement(14638..15303)
                     /locus_tag="PKM87_05455"
     CDS             complement(14638..15303)
                     /locus_tag="PKM87_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29551.1"
                     /translation="MNGRTTSYSDREIIGRWAILKRNPAIDHILAGRGLAPTGGEGVI
                     GYFYVDHEEGISVRIHALCRIEPGKLPHIAANFEDHGEDCVLRYDEFGEFRLLSTEEA
                     NNLLLSDDQRWYIFEDQRWFIYYDPEKLHEIRNRVDLDRFRAAGYFDDVSVILLARDQ
                     ERIPEVVWVRLEELSADGKSFQGILLNEPDMDFGVHEGDMLTVRFAEHEEGRFLVAQT
                     GPA"
     gene            15603..15926
                     /locus_tag="PKM87_05460"
     CDS             15603..15926
                     /locus_tag="PKM87_05460"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HNQ29552.1"
                     /translation="MPGFDGTGPRGSGPAAGRGLGPCGRGMAFQRAYPFGYGCGFGRG
                     RGFGRGYRMGYRLTYSAPAPLNTDAQKQFIEAEIRRIDAEKAELEQMLATLGSCQEEG
                     GAAEE"
     gene            15893..16186
                     /locus_tag="PKM87_05465"
     CDS             15893..16186
                     /locus_tag="PKM87_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HNQ29553.1"
                     /translation="MPGRRRRCRRIGFQPSFTWFGPATAGPAYPEEVLLSFDEFEAVR
                     LKDLEGLDQSACAGMMDISQPTFHRLVVSARRKIADALTHGKILKIEGGHYRF"
     gene            complement(16358..16741)
                     /locus_tag="PKM87_05470"
     CDS             complement(16358..16741)
                     /locus_tag="PKM87_05470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNQ29554.1"
                     /translation="MAHSSCPFCSPGKSDILYRNAHWYARWDAFPATRGHLLVVPFRH
                     VTGYFDTTAEEQHALPEMLIECRKILERQFSPDGYHIVINVGEAAEQKVMHCHVHMIP
                     RYLGKPNDPPGGIRQFILGDHGGEV"
     gene            17302..17541
                     /locus_tag="PKM87_05475"
     CDS             17302..17541
                     /locus_tag="PKM87_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29555.1"
                     /translation="MRYLPIVLLGIGISLYVLLFLLVTYPATCANFERLSGGLYTCGL
                     GPGAYLLAFGIGLALFVWLYLLYSALMSSLEYSWE"
     gene            17797..18573
                     /locus_tag="PKM87_05480"
     CDS             17797..18573
                     /locus_tag="PKM87_05480"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNQ29556.1"
                     /translation="MNESIGWIGGGRVARILLSGWKEAGMTLENIVVSDPDGMALDRI
                     IQDYPGVSVIKDGDAVAAGQDLVFLAIPPAAAETVLPAIKPVLKPGTIVISTIPGLSV
                     DQTSRMLGGFDRIVRIIPNAPSMIGSGYTPVFFPPSFLRQDSEPVEYLMESISHIIDV
                     QEEHLEAYAVISAMGPACLWFQLYELVAIAESMGISRELASDAVCKMAEGAVQMMTWS
                     TLSPSQMMDLVPVHPLAEDEAAIREIYWKRLPDLHKRLKD"
     gene            19459..19689
                     /locus_tag="PKM87_05485"
     CDS             19459..19689
                     /locus_tag="PKM87_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ29557.1"
                     /translation="MLLSELQREHDCIEYGNLRDSQGRYDEAIAAYDSALRIDPDDAD
                     ALFDKGETLEKMGNLLEAKKFYDRAMALYNGY"
     gene            19795..19983
                     /locus_tag="PKM87_05490"
     CDS             19795..19983
                     /locus_tag="PKM87_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29558.1"
                     /translation="MLQGYPDQGCCSGDHDGCIPIRNSSTMTDRAMQGISALRWSGDV
                     TPFRTVAVSFMRIIGFLL"
BASE COUNT         4659 a         5563 c         5603 g         4613 t
ORIGIN      
        1 gcacactctt ctcatccgaa ccgacagatt tgctgatcat ggagagattg tctcccgtcg
       61 ctacctgttt catgagatct gctactatgt tcatactgca ggagaatacg cgatggaact
      121 tatataccta tgtgtaaggt caattggttg acgaaaatta ccacagcatg cagaggtatc
      181 tcacgatgca acccgggaaa tcctcgcaaa aataagggag gagtccgacc atcacctcac
      241 gtatatgcgg cgtgaggtgg tgctctcccc gtgcgtattc gccaccttga gggaaaccga
      301 atacgtcccc ggaattatga acgtgtggga cgggctcctg atagtcgagg tctggccatc
      361 gccaaagttc cagtcccacg aggtcgggcc attgaaggac atgtcagtaa acgcaacagt
      421 catcggggca aacccaatgc ttttactcac catgaagaag gagattggag cccggcccgg
      481 catcagcgga ggcgcaggcg ttgcagtggc cgtgggagcg gcagttcccg aagaagacgt
      541 ggtgacgggc aggaacacgg tagacgccga tctcacgaag acataggaag agtgcgaatt
      601 gctcccgcca tggttgaaga ctctcagggt gaccgtgtaa gtcccggtcc cggtataggt
      661 gtgcacaggg ttctgctccg tggaggtggt tccatccccg aagttccact cccacgcaag
      721 tggcgtcccc atcgagcggt cagtaaaggc aacggtcaac ggagcaatac cagacatcgg
      781 cctcatcgtg aagaatgcca tgggaggtgc accggatgac gacacctgga tgtatcctgg
      841 ctttgtaact gtctcctgtc ccgcggcatt gccaaccgtc agcgagacgg tgtaggggcc
      901 ggggaaggga tacgtgtgat cgacatccgt gctctcgttg cgccattcat tcgtcccatc
      961 cccaaagtcc cacagccggg aggtgacgag gcctgtcgag gtgtcggtaa aggcaatgcc
     1021 aagtggcgca ctccctgaca gcgggctggc ctcgaagtca gggaccgggg gcagggactg
     1081 cgtaacattg atgaaccctt cctttgtgag cgtgctgttg ccgtacgcgc tcgttacggt
     1141 cagagtgacg ttatagaggc cgggggcatc aaaggtatga tacagccagc tcccgctccc
     1201 tgttgtcccg tcaccaaagt cccagaggaa ttcgtcataa caccaggaat atggaaccat
     1261 ctcccagaac tctacctcaa ggggcgcatc gccctccatt gggtatgcat tgaagtctgc
     1321 ttccggtgca gggggatcga cctggatgta cccctctttc gtgacggtgt ctgtctcacc
     1381 ggtcctgctg acggtaaggc tcaccgagta gttcccaggg gaggtgtaga tgacaaccgg
     1441 gtctttctcg gccgagaatt cgttgcctaa atagggaatt gcctgtttcg ttgcggcatc
     1501 gcgcaacagg tagtacgagt ctttctggaa cacccatgac catgaactcg ggcttccagt
     1561 tgaagtatca taaaactgga cggtgacagg gactgcccca atggtgaggt tggaggtgaa
     1621 attcgccttc aggggctcat cctttgtgac cttgatatat ttctccctgc attctgtgct
     1681 cgacccggcg gagttcgtga ccgtaagtgt cacgttgtag gttccggccg agacgtacgt
     1741 atgtacattt tccttcttgg tcgaatacgt cccgtcacca aagtcccagt aatacgagag
     1801 atgacctcct gttgactggt ccgcaaatat gaccgagagt ggttcatcgc cgcttcgtgg
     1861 aatcgcgaag aaatcggcga cgggcggttc cggatcagga gtcgtcgcaa cccatacctg
     1921 gcaccccgca aacgggtttg cggtcccgat aatcatcccg tcatccgtcg cacgcatcgt
     1981 ccggagcccg tagttgtccg ggtctccaag accgttcagg ttcatcggga cccagtgaac
     2041 accgtcctgg ctcctccaga gatccgctcc cccgaagtgg attgcaagga gcttgatgat
     2101 caaagcgagg tctacctctt ccatgtgttc aaggtccata tcgcggagca cttcgagctc
     2161 ttcggaaaac tccccggaca tctcccctct ttcgagcgat tcgagtgcct caaacagaat
     2221 atcagtgcct ccagggacct cttcgatgaa tcggccaaag gagaagatat caaaggaacc
     2281 gaggtacagg tcgtcgtggt actcttccat cgaccatgca tagaggttga aggggttcgc
     2341 atacccggac ggccacccgc tcatcggaag gccacgcggg taggtcggga tcctgggatg
     2401 gatgttcccg acaatcaggt cccaggaatc atcggaatca aggcggatga ggtcgaaccc
     2461 cttgacaccc tcggtcccgg tgccgaacgg gaggttcatc gaacccacgt agaggtctcc
     2521 atcgtactct ctcattgtcg cggcaaggac gttccaggcg tccccggccc cgccggatac
     2581 caccttcacc cagtcgtccg ggccttcagg tgatgccgtg gtgctcctcc atacctcaaa
     2641 gccgttcgtc tccgaaggac cgatggggtt cccggtccct gcatagaggt atccgttata
     2701 actttcgagg gatgaggtct ggaaatccgc caccttcacc cagttgtcct cgtggggaga
     2761 gggcgaaccg gtggcataaa tcccttcacc tgttcccatg tagagcctgc cattgtggga
     2821 gatcatggcc ctcgtgtccc ggctgggaat actcgaggtc ctgattggca cccaggtatc
     2881 accatcggtg gaaccgacga caaggatagg gccgaatccg aacccgactc ctgcgtagat
     2941 gccttctgtc ccattgacat cggaaaacgt tgtcatggcc cggtatccga tcccttcagg
     3001 ataagtgtac ccgttcgcgg gattgatgaa cgtggtggcc tggtgcacgc gttcccagga
     3061 gtccccggaa tacctccata tctccgcccg catgtcattt gaccactcga tcacgtcctc
     3121 ctgggatggt ggcgtgtggt tgggaagcac aagcggcggc gggggcgatc cggacggggt
     3181 tgttaaaaag gagagggtcc agttggcggg gaatgcccct ctcgctttca ttgcctgggc
     3241 aacaaaatac gggatgttcc ggcctgtccc cacgtacagg tcgccgttga actcggcgac
     3301 tgcccacgcg tagttgttct gcggatcacc aaatccgccc tcgcttatcc ttgtaaaatc
     3361 cgggagaggc aacacctcgt cggcagaggc aaccgtgcag acgaggagaa tcccggcgat
     3421 gataagagct gcatatccgg ctcctttccc tgtgatcccg atctgattcc ctggtttcat
     3481 gaaaatcccc tcctcccggt cctgggcggc tcatgtgtcc gggaatcgcg gtccgtccct
     3541 cgctgctcct gtccgggatg caatgatata gggaatatta accccgggac cgccggggca
     3601 ggtattctcc tgtcctcttt tgactgcccc gcaatgatcg cggtatgatc tcttccttct
     3661 cccgctattc aatggaagga tttttttgga attgtgggtt ccccgatatc tttctgatcc
     3721 tcacgcaccc gcacactacg tgttcacgga actctcgttt ctccgcactg tttcgttcgt
     3781 tgccaccttg accgaaaaca ggtaatggtc ttttccaggt tacaaataat aaatgtttcg
     3841 ttaaccctgg caggatgcgt gtctcctgct gcgcgttttt tccttcaaac ggcgattctg
     3901 gagtcttaat ccgatattcc gccagaacgt gtatcccggc acccattcag agcccaatga
     3961 tcttttacgg gtgatgcctg gcaatttttg aaaatgaaaa tggtgtggtc cattttcatt
     4021 ggaaaaaaat aaacccgtga gtcaggagga tgaggacatt atacagactt tgctgcgagt
     4081 tctacgtcct cttccttgat ggtctttctc cctgcatgca ttgcaagctt gtttgcttct
     4141 ttgacaagtt cggcaatgta gtcttctgcc tttgcgacaa gagcagcagc ggcgtcactg
     4201 ccgacacgct ctgctccact ctgcttagca atcctaacaa ctgctgcaat aggtaaatct
     4261 gccaccttga ttacctcaac agtataaaga tttcaggagt atttatatat tcccttgttt
     4321 ttagccgttg aatacaaaaa aaatctgtca agaggtgacc gttttccaag atctgatatt
     4381 cctgttcgcc accaagccca cttgttcctc tttttctctg tcttcgtatc gggccatcga
     4441 cacccggaat tttcagtcaa atctcgccgg tgaggatcgg gcatattact cacaaagaga
     4501 caccccgggt cactctccct ggtggtagga tctccctgca tgcgatctcg tttatcctgc
     4561 aaatagcggg aggttatgcc gggaacacag ccgcatggaa tgcgcggcgg aaacctgcga
     4621 acggcatttc ctccagggga gtgttctgct gctgcacttg aaattcccct ccctggtacc
     4681 cggaagtgac gacgtgtgac cagcagttcc tatctcactg gcgggatcag ctttatcacc
     4741 ttttactgcc atcccgccca ttatgccctg ctttattatt cttggaaaac tgagcgaccg
     4801 ggccatcgag agaatgaagg atgcaaaaga ccgggatgcc gagacaaaga agatcatcga
     4861 agatgcgggc gggaccctta ttgcccacta ctacaccatc ggccgctacg atgttgtcgc
     4921 aatcgtcaac cttccatctg aagaggtcct cgcgaaagtg ctgatcggga taggcaaatg
     4981 cgggacggtc agcaccgaga ccatgactgc gctcctgcct gaacagatgt acaggatggc
     5041 aaaggggacg tgagcgcact cccctggaat gcttggcaat ggtctgttcc tgcaggacgt
     5101 aatcgcgtta ttccgggata aaacgtctgg ggggatgtcc acgagccgcg gggaggttag
     5161 acaccgcatt ctctcgttcc acggctcgcc gctaatcaaa gcacaaaaaa ctgaaaaaat
     5221 gagcgatgca gtggctggag cccccctccg cagtaaatga gtgtgtgaaa aaattataca
     5281 aaaggatagc tatcgaaagg cgattcatgg aacgggtagc catcgaaggg atagcccccg
     5341 ggcgtgtacc cgtcatacgg gtagccgtca aagggatacc cgtcaaactg gtatcccgag
     5401 aatgggtagg tgtcaaacgg cgattcatgg aacgggtagc catcgaaggg atagccccgg
     5461 ggcgtgtatc cgtcatacgg gtagccgtca aagggatacc cgtcaaactg gtgtcccgag
     5521 aatggatagg tgtcaaacgg cgattcatag aacgggtagc catcgaaggg attgcccccg
     5581 ggcgtgtagc cgtcatacgg gtagccgtca aagggatacc cgtcaaactg gtatcccgag
     5641 aatgggtagg tgtcaaacgg cgattcgaaa tttcctgaaa cgaacgaagc ccccgccgaa
     5701 catgtgaaga tggcaaggac taccatcacg attccggtca gcctggtgaa gtttatcatg
     5761 ttcatgtgcg taacagcaca attaaaatat ataaatattg accattccct ccagctgaat
     5821 ttaccgcctc cattcccttt caaaaccaag taagagaaat ccgggaattt ttcatcaaca
     5881 tatctctatg ggaaaaaaat tcccgctcct tcttatggag gttaaaaata aagagaaggg
     5941 aaggaaaggg gagaatatcc gttttttcgc atgcctgtat acccgccctg gtctgtagtg
     6001 ccctgcttca tgtcaactcc ttcccaatcc tgatcacttc ccgcacgagg agatcgaagt
     6061 catcgtttct gctgcgatgg ttagtgatag cgacatgcag aaaaattctc ccccttatgg
     6121 tgacggtaga tggcaccgct atcccctgct cctggatctc gatctcgatc tgcttgttta
     6181 tctcgttgag tcttccatca gggacgcccg ccacggtgaa gcggaagttg acgacgttga
     6241 gggagaccgg cagcgccagc tcgagttccg gggccgactc cgcctgccgg gcaagatagt
     6301 gggcctggtc tatgttctgc tggatgagcc gcccgtattt cgcggtgcct tgctctttga
     6361 aagtcatcca tgccttgagt gcgtggaagc cgcgcgagag ctcgaaccca tagtcagaga
     6421 gccatggcac gtctataccc gtcagccccc ggtctccctc tccgtgggcg aggtaacttg
     6481 gagtcagtga gaacgcccgg cggtggtcgt cggcgttccg caccaggatg cagccgatcg
     6541 gatatgcaag gtacatccac ttgtggagat cgaacgccag tgaatccgcc cgtccgatcc
     6601 ctgcgacgag gtgttttgaa cggggggcga ttgccgccca tgcaccgaac gccccgtcga
     6661 cgtggaacca gcaccgctct tttgcggcga tatccgcaag cgcattcatg tcgtcgacgg
     6721 cgccggtgtt cgtcgtgcct gccaccccaa tgatgcaaat cgggtgacac ccgctcttcc
     6781 ggtcgcgttg aatcgcctct tccagggctg caacatcgat ccggagcaac tcgtcgatgg
     6841 aaaccctccg aagtgactcg ctcccgaagc caaggagttc tactgccttg tcgatcgagg
     6901 aatgcgcctc ctccgagcag tagagggtca tccggcgggt ctgatctgtc attcccttcc
     6961 cccgcacatc gaatccggcc agcgtgttcc tcgcaaccgc gagtccgatg aggttagatg
     7021 ccgaacatcc actcgtgagg aggccgctcg cggtccgggg gtagccgagg aggtccttgc
     7081 accagtcaag gacctgcctc tcgacgtagt tgttgctcat gtaggagaag gccccgctaa
     7141 cggcgtcggt tgcggccgcc agcatctcgg catacatccc tatcacggtc cccgaccccg
     7201 tgacccatcc ccagtacctc gggtgggcgt tgcccaactg gtgggggagg atataccgga
     7261 ggtattcttg gtatacttcc cctggaggca tgggtcctgc cggcggcggc ccttcgaagt
     7321 gcgctttcac gtgaggcggt gcatgctgcc agaccgggcg ttcccggagt gtctcgagat
     7381 agtccattgc gtcatcgacg atacggtggc cgagggcacg catggaatcc cagtcacccg
     7441 ggtccagcgt ctcttcctgg ggaatatctg cctgtgggtt catggtgaaa ctccggtctg
     7501 ttggcgtcga tgcataggcg ccagccgact cctcaatttc ccgctacagg tattggggaa
     7561 ggcaccgaat attgtttatg atacaaaggc gttattgtct ctcaaatccg ggattatccg
     7621 caatcccggc cttttattga gtgaaaccgg ctgaaccgga tcaggctgcc atcggaaaca
     7681 tcggcagatg cgccgaaaaa aaggcaaaga tgacgggaaa tccccgggga ttatacccgg
     7741 accccgttcc agaactccgt cgcgtttgca agttcttcga gggtgtccgc ccacttcccg
     7801 gtaaacgagt ttccgtcagg ggacagcgta aactcgaata ccccggtcac cgtctcgttc
     7861 tcgtaatagt ccacccaggt gccgaccagg cgggatcccc cgaaagttcc gttgatcgtg
     7921 ccattgcttt cagaatacat gccgatcacg gctgatccgg cctgctccag gaacatgaga
     7981 tcagggtgct ctccctcctc gtcgtaccac ttggtattcc aggtcccgga aaaggacgca
     8041 gttgttgcaa tcgggacggg ggttgcgggt acggtcgtgg cagtaggagt gggggtctgt
     8101 gcaggagcag gggcactcgt gcagccggca gcgagcacca ggatgcaggc gaggcatgca
     8161 aggatacatg tagtggtgag tttcatttga aatactctcc aaaaagttga ttcttaccca
     8221 tcggagccgc ggtatttaat ctctcggaaa tgaatcccaa ggcatcattc ttaattgaga
     8281 cggcattcac aaggttagtg cggaggagga attcccggaa actactcctt taaccatcac
     8341 atttcttatc tcttgaaaga ggatgattta aatcaggaac atactatgaa tcaattgtaa
     8401 ggtatcatac acatacaccc gcatcgctga ttccagggca tcacctgttc tcccaatcac
     8461 cccgtgaaga aaacagattc tggacacctt caccctgcac atatcatatc cttaaatacc
     8521 ccttaaaccg agtctttgcc cgatagaaat ggaagatatt accatcgtgg aagtgccatc
     8581 ccagcaggta gtgggtatca ggaagagtgg taactacacg ttgatccctg aacttctcat
     8641 gcagcttgcg gagtatatca tggagaacaa tattgcgttt gcagggatgc cgatgttcat
     8701 ctgccacgag acctccccgg atgcggtgaa ggaggcaaac gagaaaggga ctgcgcttgt
     8761 cgaggtcgca tggccggttg caggaaaagt ctccggcagc ggtgacataa aggtctacac
     8821 cctcccgggc gggaagatgg tgcggaccgt ccacaagggg ccatacgaca cctgtgaacc
     8881 tacgtacctc gcactcttct cctggatgga gaagcatcgt ctccaggttg cagggccgat
     8941 tcgcgagatc tacccaaacg acccccggga agtgaaacct gaagatatcc tcaccgagat
     9001 cctggtgccg atacgatgac cattacgtcc ttaatgtatc aggagtgaga tctcataggg
     9061 gtaccatcac agagccaccc tccacttttt caaacgaatc tcatatagag tgtttgtcct
     9121 gcattccctg gaatcccctt tcatctcaag ggattgttca cgcggctatc ttgcagcccg
     9181 cctccccatt cccgcgttga aataaaaacg ggcggaccgg ttcttcgtca ccattccctg
     9241 ggccatggac aatatgcgat gtcccgacaa cctgaccgga gggaaaattc cctgggagaa
     9301 ttgtcacctc ttgtgaaaag gaagatacgc gggatggcag gtcccattag ccttttagcg
     9361 ttcgcattaa aacgggaatg agacagatga atcatcgcgg aaggcattga catgagtgat
     9421 ggagcgcacg gtgtgtagtc tggaggagtg aaaaaatcat ttcacgagtc ctttagggaa
     9481 atcgtttttt ggatgtcgga tggaactgtc tctatcttcg gcctgttcgc tgggatggtc
     9541 gcaggaaccc agtccagcga tgtcatcctt cttgcgggtg caaccgcagc aatcgctgca
     9601 actgtatcaa taatggccgg tgtcttcctg gatctcgaat ctgataagga tgaagccagg
     9661 gtggaggcag aagagcgtgc cgccgagatc ctccacaatc ctgacagagc ggttgcgaaa
     9721 ctgatgaacg atcctgaacg ctccgacctc tccaagggca agcctcgatg caatccatgc
     9781 cgacctccgg aatgatcccc ataccatcca gaagttcgag gatgcggttg catccgaaga
     9841 agtcccggcg ggtaagaaag cctctccctg cgtccacgca tgctggatgg gcatcgcgga
     9901 cttcgtcgcg gcgatgacac ctgtcatccc ctttgcgttc ctccccccag aaatgacaaa
     9961 gattgcctgc gttgcaggaa ctgccacact ccttttcctg cgtgggattg cccgtgcgag
    10021 gcctggaaag cgtccggtcg tgcggacggt gcttgaaacc atggctatcg caactgcccc
    10081 gggtgttgca gggctgggcg ttggcctgct gatcacgtga cgaaagtggg cctaattccc
    10141 cacccttgaa tatttccatg gatagtatgg ttcggtgcag ggaatcggca taatcagacc
    10201 attatgctgc tcgcacccag aagtggtcat ctcttgccgg gatccttttc cctaaaaaga
    10261 agacgtgatc ttctcgagcg ccttcttttt ccggacgttc tccttatgct cgcgaacgcc
    10321 ccttaaagag accttccagc tcctgcagag cgaccggacc tcctctgcga ccgcatccgg
    10381 gtcggccccc tctctcccca gcatgtggaa catgacgagc cttggcgagt cggtcgtgtt
    10441 caggacctgc acggcgagcc tctcgcagtc gacgtttctc gctgcaaggt cgatgatgcc
    10501 aagcgacagg aaccctgcct ccgtcggctc gacgataaac tggagataga aacagccttc
    10561 cggtgcaggg ttttcgcggc ccacgttatc gtgatgcccc ccgcaggagc tgcaggtgta
    10621 tatgtcgggg aggtcgttga gcgtctcgac gagtgtccgc acccgagggt cgcactcgac
    10681 cgggagggag aggactgcgt agatcagctc ctcgcgtgtc cggtcggccg agactttctc
    10741 gtatgttctc cctctctcat cccgggagtt catgtcccct tctccctcca atatcccttc
    10801 cacctgttct tattggatgc caggactctc caggtacctt tcattggaac aagtccccgg
    10861 tcgtcctcac ctctcaagcc tggcatctcc gcaccaggag gttgcattcc aacctgcatt
    10921 gttctgtcgg tctgttgggc cggtgcacgg gagtgtccat gggacgagag atgaatctgt
    10981 gtgcagtcag agaccctgga tgtgtcagcc tcagggatac tcgctctatc gtgtcttttg
    11041 atctggcctc gaaaatttgt gcatgtggac gagaacagta aacagcaggg gtacgatccc
    11101 aagtgatgat atatatcaat accaatctgg atagcatatg gaggacagta tccgtgttct
    11161 ctgcgtcgat gatgaaccat ccctcctggc ccttgccagg atgttccttg agaagtccgg
    11221 gggatttttc gtagggacat ctacatccgc tgaggaggcg ctcgaccgcc acgagatcat
    11281 gtcctatgat gccatcgtct ccgattacca gatgccgggc atggacggga tcgagttcct
    11341 gaaagcagtc cgcctgcggt tcggcgacat cccgttcatc ctcttcaccg gcagaggccg
    11401 cgaagaggtc gtgatcgccg ctatcaacaa cggagcagac ttctatctcc agaaaggcgg
    11461 cgatccgaag gcgcagtttg ccgaactcgc gcacaaggtg cggcaggctg tcaggagaaa
    11521 gaaggcggaa gtctcgctga aggagagcga ggagaagtac cgcatgctgg tagaacacaa
    11581 ccgggacggg gtcttcatcg tccaggacgg gaaactggtc ttttacaaca atgctctcac
    11641 cggcctctcc agatataccc cagaggaact cgagggcatg catcttgccg acctgatcgc
    11701 gcccgaggat cgcgagatgg tcctctcaag ggcaaaggtg agggcagagg gaggattggt
    11761 cccggaacgc tacgagttca ccctcgtcca caaggacggc atccaccgga gtcgcatgcg
    11821 gatcagcacc tacctcggaa gttatcaagg acgccctgcc tcgtttggaa ccttttacga
    11881 cgtgaccgag gaccggagac gcgaggcagc cctccgcgag agcgaggaga agtaccggac
    11941 aatccttgag aacatccagg atgtctatta ccgcaccgat acacatggca acctcatcat
    12001 gctctcccgg tcaggatatc gcctgctggg atatgaccgc agtgagaaca ttctgggatc
    12061 aagccttgcc gggacattct atgccaatcc cgaagaccgc aggaaattgc ttgaagcaat
    12121 cagtgagaaa ggagccgtgc atgactacga ggtgaacttc cggcacaagg acggctccat
    12181 cattacggtc tcggccaaca gccattatta ttatggaaat aacggcaaaa ttgcggggat
    12241 cgaggggatc ttccgcgaca tcacggagca aaaacgcgcc ggggaggatc tgaaacgatc
    12301 cgagaacctg tacaggacag tctttgaaca caccggtacc gccaccatcc tgataggccc
    12361 cgacacgacc atcctcaaag caaacggcga atgggagagg cttaccggag tcccgctggc
    12421 agcgcaggag aatatcctga gctggaccgg gttcatccac caggatgacc gcgaacggat
    12481 gaaggagtac cactatgcca ggcggaagga ccccgccgcc gcaccgggca tctatgaatg
    12541 caggctggtc gatgcacacg gtggcatcca ttatggcttt gtccatgtcg ggatgatccc
    12601 agggactggc aacagcatcg catcgctcgt ggacgtgacc gcactcacgc aggcccagga
    12661 agatctcaag gcaagcgaaa gaaagatccg cgagatcctc gaccaccttc ccgacctcat
    12721 cgtagtgcat cgcgagggga atatccttta cgtcaacccc gcaatgaccg agtccatggg
    12781 gtggcagccc gatgaggtgg tgggccaacc aatcctgaag ttcatcgctc ccgagttcca
    12841 tgccgcagta caggacgctg cccgcttgcg ccttgaatcg gggattgaca gtccatatat
    12901 catcaatctc attaaccggg acaaaaaacc cctgacggtc agggtccgcg ggactgtcat
    12961 cagcgtatcc gggtctcctg cgatcatgaa catcctgacc gacatcacca ctgtcaggaa
    13021 tgccgaaaaa gccctcgagg agagcgagag gaaataccgt atgctggtgg agaataccaa
    13081 tgatatcatc tacacgacgg atctcgcagg gcaaatcacg actatcagcc cccagatccg
    13141 acggtatggg tactccccgg aagagatagt atccgagaac ctgacatcat ttattgtcga
    13201 agaagacctg cccagggtga ttgctgactt tgagaagacg gtctcaaccg ggcagtcgac
    13261 cctcacgatc ttcagggtaa gggatagatc cggcaccata cactggttcg aagacaatgg
    13321 gagtgcattg actgatgcat cagggaatgt gattggaata tcgggaattc tccgtgacat
    13381 cacggatcgc aggacggccg acgacgcgct cgtgcgtgca aaccgccagc tgaacctgct
    13441 cgggggcatc acgcgccatg atatcctcaa caaggtctcg gtcatcctgg gataccttga
    13501 gattgcaaag gagagcgtga aggatccgga gctgggcgga tacctggaaa aggtcgagtc
    13561 tgccacccgc gcaatccgcg aacagataga gttcatgagg ttgttccggg atatcgggac
    13621 ccgggctccg gagtggatct ctcttgattc cgcacttcct caccgtcatg tccctgccac
    13681 catttccttc catgcagagg taataggggt cgatatcctg gcagagccaa tggtggaacg
    13741 ggttttctac aaccttctcg acaactctgt ccgccacggg gggcgggttt ctgcgatcag
    13801 ggtatctgca tatcgttctg gcgacagtct cgtcatcgtt tgggaagaca acggggttgg
    13861 aatcgcctct ggggagaaag agaggatctt tgaccggggc gtggggaaga ataccgggct
    13921 tggcctgttc cttgcgcggg aaatcctctc cctgaccggg atcaccatca gggaaaccgg
    13981 ttacccgggc acaggtgcac ggttcgagat tacagtgccc cggggaaatt tcagaatggc
    14041 tgcagagtag ggcacggggg atgcaagggt cacggaaggg gttttccatt cgcatgaacg
    14101 ccatcatcct catcggcatg cccggagcag ggaagagcac tgtcggcgtg atcctcgcaa
    14161 aggcgcttgg gctgaagttt atcgatacgg acatcatgat acaggagcgg accggaaagt
    14221 tgctccagga gatcctcgat tgggaaggcc cccgcggatt tgagcggatc gaggaggaga
    14281 cgatcctctc cctgcaccct tctcacgcgg tgattgcgac aggtgggagc gtggtatgca
    14341 gcagggcggc aatggcgcac ctgaaatcca tgggagtggt ggtgtacctg aagatatcct
    14401 acacggaaat ggaacggagg atcagaaaca tcacgacaag gggcatcgtt ctcctccctg
    14461 gccagagctt gcaggcgatg tacgaccagc gggctccact ctaccagaaa tatgccgatc
    14521 tcacgattga atgtttggac aaggactttg agaccgtggt cggaaaggtg attgccgggc
    14581 tgtgaagaat ccgcgtgatg acggatatcc cggtctttta tgagggggac atctcattca
    14641 cgccggtcct gtttgcgcga caaggaaacg cccttcctca tgctcggcga aacgaaccgt
    14701 aagcatatcc ccttcatgca cgccaaaatc catatcgggt tcgttcagga gtattccctg
    14761 gaatgatttc ccgtcggccg agagctcttc caggcggacc cataccactt cggggatccg
    14821 ttcctggtcc cttgcgagca ggatcacaga tacatcgtca aaataccctg ctgccctgaa
    14881 cctgtcgaga tcgaccctgt ttcgtatctc gtgcaatttt tcaggatcgt aatagatgaa
    14941 ccatctctgg tcctcgaata tgtaccatcg ctgatcgtcg gataacagga gattgtttgc
    15001 ttcttctgtc gaaagaagcc ggaactcccc gaattcatcg taacggagga cgcaatcctc
    15061 accatgatcc tcgaaattcg cggcaatatg agggagtttt ccgggttcga tccggcacag
    15121 cgcgtggatg cgaacactga tcccctcctc gtgatccacg taaaaatagc cgattacccc
    15181 ctctcctcca gtgggggcca gtcccctgcc ggccaggata tggtcaatgg ctggatttcg
    15241 ctttaaaatc gcccacctgc cgatgatctc ccggtccgaa taggatgtcg ttctcccgtt
    15301 catagtgcgt cgttacatcc tcttcatggg aagcgtgaaa gtggtttcgg aatatgcatg
    15361 gacagccaga gcctgcccct gttagatgag agccgggcat ggtagaatgg ccggcttctt
    15421 cagttgagaa gggaatcctg attgacaggg tgtcgtgaat atcaatgcca tgatacgaaa
    15481 agagaaggtg aaaccttccc cccttttcac gcattttggg aatgcaccgc gattcgcaca
    15541 tgtgccgaaa cccaaaccat tatgccatta tgaatatata ttcaaaaagc gaggcgaaaa
    15601 agatgcctgg atttgatgga accggcccac gcgggtctgg acccgccgcg ggaagggggc
    15661 ttggcccctg cggcaggggg atggcgttcc aaagagcgta tcccttcgga tatggctgtg
    15721 gattcggacg tggtcgtggc ttcggacgtg gataccggat ggggtataga ctcacgtatt
    15781 ctgcgcctgc acctctgaac acggacgcgc agaaacagtt catcgaggcg gagatcaggc
    15841 gcatcgatgc agagaaggct gaactcgaac agatgctcgc cacgcttgga tcatgccagg
    15901 aagaaggcgg cgctgcagaa gaatagggtt ccagccctct tttacctggt ttgggcccgc
    15961 aactgcaggt ccagcctatc ctgaagaggt cctcctgtcg ttcgacgaat tcgaggcggt
    16021 gaggctcaag gacctcgaag gcctcgacca gagtgcctgt gccgggatga tggatatctc
    16081 gcaaccgact ttccaccgcc tggtggtatc ggcacgaagg aagatcgccg acgctcttac
    16141 ccacggaaaa atcctcaaaa tagagggcgg acactaccgg ttctgaccgg acgagtcgcc
    16201 ggaattttca aaacgacgtg tatccattcc cggaacgcac gcgacaccgc acgaaaatag
    16261 tagagacgtt ttcgcaggga acaagaggtt tcttctcatt ctcaatggtc tggcaacgtt
    16321 ccacgaagga gcggcaagcc catggtgttg aagtccttta cacttcgccc ccatgatcgc
    16381 cgagaataaa ctgacggata cctccaggtg ggtcattggg tttaccgagg taccgcggaa
    16441 tcatgtgcac atggcaatgc atcaccttct gttctgccgc ttccccgacg ttgatgacga
    16501 tatgatatcc atcaggtgag aattgtctct ccaggatttt tctgcattcg ataagcatct
    16561 ccggcaaggc atgctgctct tcggcagttg tatcaaaata cccggtaaca tggcggaatg
    16621 gaacgaccaa gaggtgtccc ctcgttgccg gaaacgcatc ccaccgtgca taccagtggg
    16681 cattgcgata caggatatcg gacttgccag gcgaacagaa gggacaggat gaatgtgcca
    16741 tctcatctcc ctcacaatcg gttcgatccg gttttactgt tatttgtcag gcgaatgcac
    16801 cgcaattctg ctggcatgga gtatgatgag cagcctttaa cgataaattc cccccatttt
    16861 ccccctttga tggaagatat accttctctc ttcccgtttg cccccgcata aagggggatc
    16921 gggcatccaa cccggcatcc agatctaatc gggttatgag tgttcccacc acctttcagc
    16981 agatgtaaaa gcaattctcc cgcatgtcat gaacgagacc cccaccatgt gttatcctcg
    17041 tcacggcggc acaccatttg cttattcaac gaaacgatca ttcatagttc agggttcccg
    17101 attcaagaga attgtgcgaa ggaggcttcc cggctgatgg gtgcgcatgt gcatcgccca
    17161 ggtgacatac attcgtctta ccgggttttc actcgcacac gagttccgaa tggaaccttc
    17221 tcttgattat tttacagatt cctttatttt cccctgcacc aacataacga tgacattatg
    17281 agaatcggga ccttttttcc aatgcgatat ctgccgatcg tgctcctcgg gattgggatc
    17341 tcgctgtatg tcctgctgtt tcttctcgtc acctatcctg cgacctgtgc aaacttcgag
    17401 cgcttgtccg gcgggctgta tacctgcggt cttggacctg gggcctacct ccttgcattc
    17461 gggatcgggc tggcactgtt tgtctggttg tatcttttgt attctgcgtt gatgagttct
    17521 cttgagtatt cgtgggaatg atttgggggg gagtgttccc gaaccttcac cctgcatttc
    17581 ccgggctatg aagccggcac gaatcaatga tcatttgtag actgaaggag aatgttatga
    17641 tccggtaatt tacccggcat atatgccctc tcgtcggcat gcccaggaac gtaacggttc
    17701 ccggtgagat gagggatcgt atgattgcca gatcagttga gccgggggaa tagacccgcg
    17761 ggtgcgatgc agacaggata tacaggtgtg aaaaacatga atgaatcgat cgggtggatt
    17821 gggggaggcc gggtggcccg gattctcctc tccggatgga aggaagccgg gatgaccctg
    17881 gagaacattg tcgtcagcga tccggacggg atggcactcg acaggattat acaggattac
    17941 ccgggtgtga gcgtgataaa agacggggat gccgtagctg ccgggcagga cctggttttt
    18001 cttgcgatcc cgccagcggc tgcggagacg gtgctgccgg cgatcaagcc ggtcctgaaa
    18061 cccggcacaa ttgtgatctc gacgattccc gggctctcgg ttgaccagac ctcccggatg
    18121 ctcggggggt tcgaccggat tgtcaggatc atcccgaacg caccctcgat gatagggagc
    18181 ggctacaccc cggtcttctt cccgccgtca ttcctccggc aggattccga gccggtcgaa
    18241 tacctgatgg aatcgatcag ccacatcatc gatgtccagg aggagcacct tgaggcctat
    18301 gcggtcatct ccgccatggg gcctgcctgc ctctggttcc agctatacga gcttgtggca
    18361 atcgctgaat ccatgggaat cagccgggaa ctggcttccg atgcagtctg caagatggcg
    18421 gaaggcgcgg tccagatgat gacctggtcc acgctttccc cctcgcagat gatggacctc
    18481 gtgcctgtcc accctcttgc agaggacgag gcagcgatca gggagatcta ctggaaacgc
    18541 ctgccggacc tccacaagag actaaaggac tgatatatta tcgtgaatcc ccggctccgg
    18601 ccgagtcaag cggagggtgg aaatcgcgtc ttccatggga ttacggagat gcatgtggag
    18661 gaccctttcc atcaaatttg accatcctgg aaatagcatt ttgttgccca tgtagaatat
    18721 aatcccgagg gtgaccactc attccggcga ttcggccggc ttaaggatac acttgaatac
    18781 cccgaatatc acgcgaaatg aaggatacaa aaaatcatca caaattcctc gtcaggatga
    18841 acatgcgaga cggtccggca tgttctggcc tgcacctgtg ataatgctct gctccgctta
    18901 tttcactaaa ttccgtggaa taaaatctga tacacccatg agggatatat tttcaaatat
    18961 ttttcaacac ggttcctgat accgatcatg aaagatggca tttacctggc tttgtaacac
    19021 atcttccatt catcgaggca ggaagtcgtt tcatcacgac gttcatccat ggctgcaaga
    19081 tcatagaggt ctttctgaaa ttgggaaatt catcaaattc atgtaaattg ttgggaaaaa
    19141 attcacaagg gaattgcttc accctttcca attcttttct tccaggcccc tcactcctgt
    19201 tcctcccatc ctcctgcata atggaaatga gatgacaggt gaatccataa acctgagaga
    19261 gtgcatcatc acagatcctc ctgagaatgg ccaggctggc cgattgtaag gagagagaaa
    19321 tttacttgaa tgcattgaat aatggatcag gaagcactaa tgcccggcac tcgttccccc
    19381 agatgttccc ggcaaattac tattatacca caggatcagt cagtatcagt cacaaaaaag
    19441 tgacgagggg gaaaaaacat gttgctgagc gaattgcaaa gggaacacga ttgtattgaa
    19501 tatggaaacc ttcgtgacag ccaggggcgg tacgatgagg caatcgctgc ctatgacagc
    19561 gccctccgga tcgaccccga cgatgcagat gcattgttcg acaagggaga gacgctggaa
    19621 aagatgggta atctcctgga agcgaaaaag ttctacgatc gtgcaatggc actgtacaat
    19681 gggtattgag attcccactc ctttttttag tgatttctga aagagaattt tacgggacat
    19741 cgtattctgt ccaccgaggc ttttctgaaa gacactggtg ggcgggagga gattttgctg
    19801 cagggatatc ccgaccaggg gtgctgttct ggagaccatg atggctgcat cccgatcagg
    19861 aattccagca cgatgacgga ccgcgcgatg caggggatct ctgcccttcg ctggagcggc
    19921 gatgtgaccc cctttcgcac cgttgcggtc agtttcatga gaattatcgg gtttttactg
    19981 taatggagtg tcgatccaaa agatgacaaa gatattagtc gcgattggaa attgtggggg
    20041 ggaagggttt tggattggga ttttgggaga ggattggaga gagaggagag gaatctttca
    20101 atttcttgaa gctgtactgg attggcgtgt gagatatccc cccatagtct caacgtttcc
    20161 tggttgttgc atgagtgttc cgtgatgaaa catacactca cacttcgagg agcccccgag
    20221 tgtagctcag ccgcccccat ccgatgacaa tccgccgaat atccccgctg cctcccatca
    20281 ccgcgccatc agcgcgaaca caccccatcc aacgtattca cgcgtatagg tggcgtagcg
    20341 tgttggtccc aaagtcagtg tggctctcac ctctttcgcg aactcgtcgt cgggattcgc
    20401 gtcgagccat cggcgcatgg tgagccactt ggccgctt
//
LOCUS       DAOQYR010000032        20379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_2995_length_20379_cov_8.906170, whole genome shotgun sequence.
ACCESSION   DAOQYR010000032 DAOQYR010000000
VERSION     DAOQYR010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 20379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20379
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2995_length_20379_cov_8.906170"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="PKM87_05495"
     CDS             complement(<1..1379)
                     /locus_tag="PKM87_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNQ29559.1"
                     /translation="MLGAWREAGSLGGRAGAVVLLVALALCGIVAGSEIRATPCTIES
                     PGGTADCILVLDRVSPGLSEFEVKVTLGDPATGEITAVRFPSWAGLNSKSKVPSDSVI
                     LKGSDTGRKVGPGSCEVVLATLTIRGDTPGTCGIDVEVIGMRDEVGSPYTLPGKAGTL
                     VVREPLEDTGNQESTAHPSSSPLLPQAGGPMTPLPTLDPISLIKENPCVPTDSPAASP
                     DGPQEAGSGIEEPDTDAGVTTGTLCVDSDPRGAQVFVDGQACGVTPLCLALDQGIHHL
                     SVLAEGYFGLEGEYEVPAGQVLHLPAFVMERPAKCWIIAGAGAHGSVYPSGCVEVGRG
                     GSAEFVFHADPGYRLDALQIDGVWYDAKSPVRFEGVTANHTLWGEFSPFPAPMADFAA
                     NRTEGYAPFAVRFDDLSVGDISGTLWEFGDNTTSFESSPVHRYSQPGTYTVSLEVCGA
                     GGCNLTRKE"
     gene            complement(1522..2514)
                     /gene="ruvB"
                     /locus_tag="PKM87_05500"
     CDS             complement(1522..2514)
                     /gene="ruvB"
                     /locus_tag="PKM87_05500"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNQ29560.1"
                     /translation="MDERVVSPVPEEGEVDDPAIRPDRFSDFIGQEALKETLRIAIEA
                     ARRRGEPLDHTLFSGPPGLGKTTLAHIIAREMGSSIHCTSGPVLEKPGDLAAILTLLK
                     RGDVLFIDEIHRLHTVIEEILYPAMEDFFIDVIIGEGPSARSIKITLEPFTLVGATTR
                     IGLLGSPFRDRFGLISRLSLYGHEDLARIIERSAAIMGIPILPDGSMEIARRSRGTPR
                     IANRLLRRVGDYALVRGGGVVDRDVADRALCMLGVDSLGLDELDRRIIQVIVQEFGGG
                     PVGVKTIAIAVGEDTRTIEEVHEPYLIQAGFIKRTPQGREATPAARGHLTRQWW"
     gene            complement(2521..3117)
                     /gene="ruvA"
                     /locus_tag="PKM87_05505"
     CDS             complement(2521..3117)
                     /gene="ruvA"
                     /locus_tag="PKM87_05505"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNQ29561.1"
                     /translation="MIARLRGRVVAGGDGFVILETGGVGFQVFMPRPLAHEAARSPAE
                     VVIYTHLVARDDTMALYGFLAPGELEMFRLLITVNRVGPALATAILSQMTVSDLAAAV
                     IGGDEKALTRVSGVGQKNAARIILELKDRMKKKAALLVEGRDTGADPTRSDAESALLA
                     LGFSPRDSRDAVSETLAGMDCPTVQDVIKASLRRLRER"
     gene            complement(3114..3596)
                     /gene="ruvC"
                     /locus_tag="PKM87_05510"
     CDS             complement(3114..3596)
                     /gene="ruvC"
                     /locus_tag="PKM87_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNQ29562.1"
                     /translation="MRVIGIDPGLATVGYGVLARHDGRTVPLTYSCIRTAPAVGGTPE
                     RLERIFDAISAILDEYSPGCMAMEKLFFSRNVTSALGVAEVRGVIMLAARKRGIPVVE
                     YTPNQVKQAVTGSGKADKKQVQEMIMRLLGLPDIPRPDDVADGLSIALCHVHTVRNTR
                     "
     gene            complement(3695..4657)
                     /locus_tag="PKM87_05515"
     CDS             complement(3695..4657)
                     /locus_tag="PKM87_05515"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNQ29563.1"
                     /translation="MPKGSGDGRAQVCRYGAWFLSRVALEFREEIEVVKEGRDVEAVH
                     RMRVTSRRLRAGLPLFSQCFPGKAYKKISGGIRRITRDLGDARDLDVQIGYLEALIEE
                     HRDDGGPAVDGCTILLSMKRTRREALQPKVVADCTRLEQKGVIELLEAGIAAWSGPGS
                     ILRESPPGGFPMKRSGSAIKRRAEKLESYSPMVQDPSAVEAHHAMRIAAKRLRYLLEV
                     YAPLYRKEFRPAIRQLKRLQEILGNLHDCDVWIADLQEALGKGRKPAGTPDPVLDEGM
                     VFVLKDRRKARDEYYLLLKKEWEALEAGGFCPHIREIASSGSRR"
     gene            complement(4638..6785)
                     /gene="ppk1"
                     /locus_tag="PKM87_05520"
     CDS             complement(4638..6785)
                     /gene="ppk1"
                     /locus_tag="PKM87_05520"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNQ29564.1"
                     /translation="MSGPSPVKGTAARGRERCDLLEDPGLFINRELSWIEFNRRVLRE
                     AAFQYHPLLERVKFLSIFANNLDEFFMIRVSGLHRQVEQGVLESPPDGMSPSRQLQAI
                     HQALVPLIGEQMALWKEDLLPALASFGIFVYRYGELPKEDREYLGEYFRHEIFPVLTP
                     LAFDRSHPFPFISNLSISLAVQVDDPRTGEDRFARVKVPTHLFPRLVRIPGRDRDSSL
                     REEARFVFLEDLISANLGLLFPGLDVVAAYPFRVTRDADMEIEEDEASDLLTAVEEGV
                     EMRRTGSPVRIEVVRGIPRKTCAILSRKLGVPQHMFYSQEAPLGMADLMQLCSLDRPD
                     LKDPPFAPSVPPALEREHEIFRSIRRKDVLLFHPYESFSPVIAFLRQAARDPQVLAIK
                     MTLYRVGAQSPVVKALLEARERGKAVAALIELKARFDEENNIGWARALEHAGVHVVYG
                     VVGLKVHAKVCMVIRKEKDGIVRYVHLGTGNYNASSSRIYTDIGVFSADPVLGEDVSD
                     LFNSLTGYASIPSYRTLLVAPQSIRSGILSRIGREIARHQEHGDGCIIFKMNALVDRE
                     CIMALYRASRAGVRVILQVRGICCLRPGIPGTSDNISVTSIVGRFLEHARIYYFRNGG
                     KEEILLGSADLMPRNLDRRVEVLFPVKDPELHRAIADVILPVHLRDNQKTMELLVDGN
                     YAHMPGKEGEEPCNAQKWLIAHRGCWQHAKGIR"
     gene            6956..7372
                     /locus_tag="PKM87_05525"
     CDS             6956..7372
                     /locus_tag="PKM87_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29565.1"
                     /translation="MGSNEDIMHDFVRRELKRLYPSVDGWQIKNVPKSGADSGFIISR
                     RLLGRFEGSHVLVSFDRSVTSGTVNALKEMAASNPVSGVSSPRLILMAPQGAEVAGVP
                     PEVQVLPMQSFGYEGKELVWLKRRAQVSEKVAAKSA"
     gene            complement(7369..7872)
                     /locus_tag="PKM87_05530"
     CDS             complement(7369..7872)
                     /locus_tag="PKM87_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HNQ29566.1"
                     /translation="MSGTPDDPTRRTRFLADRMLGTLCRYLRFMGYDTESASGYREGN
                     TREDTLLLKRAQGEGRVLLTMDRELARRGGEGAILLEDRDVMEQLRVLTRTGLIVPAL
                     RLNRCSLCNTLLRAAREDEVSRARHAPRSTPGMEFFWCPRCRKLYWQGSHGKDLEKRI
                     KENILKD"
     gene            complement(7853..8866)
                     /gene="amrS"
                     /locus_tag="PKM87_05535"
     CDS             complement(7853..8866)
                     /gene="amrS"
                     /locus_tag="PKM87_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNQ29567.1"
                     /translation="MHRALQWSPGEDGAVQCSLCSHRCTIREEKTGICGVRRNQGGVL
                     YATTYGKVSAEAVDPIEKKPLYHFLPGTLSYSLGSIGCNFRCEHCQNWHISRAERDAS
                     HLPDLSPGEGVRRAKAAGCASISWTYNEPTIWHEYALDMGTLARKEGLFTVYVTNGYI
                     TEEALRELSSMLTAYRVDIKSFSEEFYKKVCGARLQPVLDSAVVARELGMHVEVVTLV
                     IPGLNDSQEETGALIRWIIEHLGENTPVHFTRFHPDYHMQDRNSTPVSVLERIHRQAR
                     DLGLRYPYLGNVYPHPLESTYCPRCGALCIERRGFSAVFRNLEGNMCTRCGERIEFVG
                     NPG"
     gene            complement(8915..9076)
                     /locus_tag="PKM87_05540"
     CDS             complement(8915..9076)
                     /locus_tag="PKM87_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29568.1"
                     /translation="MLRCPSCGSRDVYCVVGGYIGQIYRCKSCGYRGSLIMECERPKN
                     GEEGEDPHL"
     gene            9122..9844
                     /gene="pyrH"
                     /locus_tag="PKM87_05545"
     CDS             9122..9844
                     /gene="pyrH"
                     /locus_tag="PKM87_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNQ29569.1"
                     /translation="MTRVVLSLGGSILFPTLEAHTLRAWAPVLARLAQSVDLFVVVGG
                     GGEARRYIRAARDTWADEAFCDEIGILVTRLNATLLIGALGAVAYPRVALTPGEAMEY
                     AGQGKIVVMGGVTPAQTTDAVAAVLAERARAALLVNLTSVDGIYSADPKEHPDARRYS
                     SMTPKELVEIVGCGGLAAGSNTVVDMVAAKVVERSRIPLLVLDGRDPALLEKSLFSHE
                     CAGTIVIGDQENPLPLEKGRSP"
     gene            9883..9957
                     /locus_tag="PKM87_05550"
     tRNA            9883..9957
                     /locus_tag="PKM87_05550"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9919..9921,aa:Pro,seq:tgg)
     gene            9963..10688
                     /gene="nth"
                     /locus_tag="PKM87_05555"
     CDS             9963..10688
                     /gene="nth"
                     /locus_tag="PKM87_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNQ29570.1"
                     /translation="MDRETACRIYSLLMKKYDDPRSPRTFLEFENPYQILVLTILSAQ
                     TTDRNVNAIRNEIFERFPEPEDLAKARTEEVEAIIRSLGFYHAKASHIIGTARSLVGR
                     FGGRVPRTMEELLTLPGVGRKTANIVLNHAYGIDEGIAVDTHVRRVSGRIGFSSHPDP
                     SRIEHDLMRLFPVEVWGNLNYLLIRHGRAVCTARNPKCSECPVRPDCAYYSLHSNCVE
                     PRGSIRPRRAHGRPETLQTHSSR"
     gene            complement(10630..11811)
                     /locus_tag="PKM87_05560"
     CDS             complement(10630..11811)
                     /locus_tag="PKM87_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNQ29571.1"
                     /translation="MENIVIAGIFLALLFNFVNGLNDAANSIATVVATRVLSPLQAVL
                     MAAFFNMAGPFIFTTVVAKTIGQGIVESGFLTTEVILVGMCAAVLWVFLSSVAGIPIS
                     ASHALVGGLIGSAIAFGGIGSVLWPSMDLFLRAVVMAAVGGICGAVVLSMTARRKGEE
                     NWPEYTALGALCGISIVIPAGVITGILPVSGILAIVIFIVVSPTLGMIAAYLLGMAII
                     RLFRKANSKWMNFIFSKLQVFSAAFYSMGHGSNDAQNAMGVITALLVAAGLLSEFVVP
                     AWVIIISGLAIALGTFLGGWRVVETMGKKITKLRPYQGFCAETGGGVVLSFVTAFGVP
                     VSTTHAISGSIMGVGATRGYSAVQWGIVRRIVAAWVLTIPLTASCAFAGFLVYRALFG
                     V"
     gene            complement(11818..12447)
                     /locus_tag="PKM87_05565"
     CDS             complement(11818..12447)
                     /locus_tag="PKM87_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNQ29572.1"
                     /translation="MGIKEWIIPQDEVFFTLFERLAAVVVLASYRIVDLVDDYTSVRE
                     KVHQIEQLEHEGDKITHEIYEQLNQTFITPLDPEEISKLASALDDILDYIKSTATRMY
                     HYGIPGTDPHMVELAKLIQLSVVELEEAVKGIRSMKNPRQIEQRCIEVNRLENLADDV
                     LHHAYDDLFRSNDAIAIIKRKDIYEYLEIATDKCEDVANVLSDIAIRHS"
     gene            complement(12516..12587)
                     /locus_tag="PKM87_05570"
     tRNA            complement(12516..12587)
                     /locus_tag="PKM87_05570"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12553..12555),aa:Ala,seq:ggc)
     gene            complement(12693..13844)
                     /locus_tag="PKM87_05575"
     CDS             complement(12693..13844)
                     /locus_tag="PKM87_05575"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HNQ29573.1"
                     /translation="MGRFAIVDDERIKKGECTDIYFSRSEAVLSASGNNPIVAMEVTA
                     SAVPGGMGILCGMEDVLSLLEDLPLDAYAMPEGSLFHPWEPVLRVEGKYRDIARYETA
                     ILGFLCHASGIATAAARIKAVAAERMVFSFGSRRQHPAIATMVERSAWIGGVDGVSNT
                     CAPAGIPLAGTMPHAFVMCYSSPAEAFQAFDRHSPPEVSRIMLCDTFCDEKRESLTGA
                     RCGAAAVRLDTPRSRRGDMRAILEEVRWELDAAGFPEVAIFLSGGVTLEDVARFRDIV
                     GAFGVGGAIANAPVIDFAMDMVEKEGKKCAKRGKRSGAKQVWEYPGGRHVTLPVEAAA
                     PPGAVPLMTPVIRRGAVLIRSTIEEARARVLARIPVFREGVMGDPSRRA"
     gene            13983..14654
                     /locus_tag="PKM87_05580"
     CDS             13983..14654
                     /locus_tag="PKM87_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HNQ29574.1"
                     /translation="MLGIIGGTSLLFARLPDLEKRVVHTPYGNSEVMAGEVALLLRHQ
                     RDIPPHRINYRSHLSALALLGVDRLVVLGSSGSLKKEIPPGSMVIPTDYFSSAAVPTL
                     HDHAICHVHPGFSPELSNHLKEIVPEAIFGGTYVQTRGPRIETVAEVRALALCGDIVG
                     MTLASEATLAIELGIEVAALCTVDNYANGLSDELLTYERIVEYSARYKDRTEEIISRI
                     IRELA"
     gene            14666..>15759
                     /locus_tag="PKM87_05585"
     CDS             14666..>15759
                     /locus_tag="PKM87_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ29575.1"
                     /translation="MKNTQTKGTSLLIERVLVNGTLQDIFVDETGVITSIGEGIARTF
                     RGEAETCIDGAGDLALSGLVNTHTHAAMTLLRGFADDMPLQDWLTQKIWPLEAHLTGE
                     DVYWGTKLACLEMIRSGTVAFNDMYFFMDEAARAVDEMGLCAVLAYGFLDLFSEEKRE
                     AECRATERLVQSIHGMKNPRIKAAVGPHAMYTVSPEGLRWVSEFAAEKEIGIHVHLSE
                     TEKEVTDAVSQWQKRPVKILDECGILTPRTVAAHCCWLDGDECSLLAKRGVHISHNPA
                     SNMKLATGRAMPYHWLKEAGASVTLGTDGCASNNNLDLFEEMKIASLLQKHSWNSQTL
                     LPAGEALAMATSAGAKALGFGRGVIAPGEPA"
     assembly_gap    15760..15859
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16334..16504)
                     /locus_tag="PKM87_05590"
     CDS             complement(16334..16504)
                     /locus_tag="PKM87_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29576.1"
                     /translation="MAAAEIYCFLLIRQQVKQWRRDFTSSRTLSSLRDGLRAGLNVMD
                     FAEIALQELALS"
     gene            complement(16551..18251)
                     /locus_tag="PKM87_05595"
     CDS             complement(16551..18251)
                     /locus_tag="PKM87_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ29577.1"
                     /translation="MDAGSYDCGTSTYPLLGYTIGEMLNRIARKYPRTDALVSVHQGI
                     RYTYGEFLGRVNDLARGLMAIGVEKGDRVGIWAMNHAEWVLTQCATARIGAIMVNINP
                     AYRTYELEYVLKQAEIQTVIVQGRFKTSDYIGMFREACPEVDTSRPGHLETEKFPFLK
                     NVIFMGDEPQQGMFSWSQIISRGQAVSKDELVAREESLDFDDPINIMYTSGTTGFPKG
                     VVLSHHSILNNGYIIGEGMNFSEKDRLCIPVPFYHCFGMVLSNIACMTHGSTMVLPSP
                     TFDALEVLKTVEKERCTALHGVPTMFIAELSHPDFPKYSMKTLRTGIMAGSPCPIEVM
                     KEVNRKMNMSEIVIVYGQTETAPGVTMTTTKDPLERRVSTVGRVFPHTEIKIIDPKSG
                     KIVPRGEIGEICARGYCVMKCYYHNPSATNATLDENHWNHTGDLGTIDEEGYVKIVGR
                     LKDMVIRGGENIYPREIEEFLHHHPKIADVYVVGVPDVKYGEELCAWVMVENGHSLSE
                     EEVKEFCRGKIAHYKIPRYVMFVNEFPMSITGKIQKFKMREASIKALGLEDASKIETA
                     "
     gene            18340..18500
                     /locus_tag="PKM87_05600"
     tRNA            join(18340..18377,18465..18500)
                     /locus_tag="PKM87_05600"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18374..18376,aa:Trp,seq:cca)
     gene            18652..18996
                     /locus_tag="PKM87_05605"
     CDS             18652..18996
                     /locus_tag="PKM87_05605"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNQ29578.1"
                     /translation="MHGAGVDSMLDNLAEIAPDLGRFTVEFPYGDILCRPGLDIRSRQ
                     IATVAALTALGNAPVQLNAHISMALNVGCTREEIVEVITQMAVYAGFPAALNGMAVAK
                     EVFSARDREKTK"
     gene            19208..>20379
                     /locus_tag="PKM87_05610"
     CDS             19208..>20379
                     /locus_tag="PKM87_05610"
                     /EC_number="4.1.2.43"
                     /EC_number="4.2.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,6,7,8-tetrahydromethanopterin
                     hydro-lyase/3-hexulose-6-phosphate synthase"
                     /protein_id="HNQ29579.1"
                     /translation="MYLIGEALVGDGPELAHVDLILGDKEGPVGAAFANALSQLSAGH
                     TPLLAVVRPNLLAKPATLIVPKVTLRKGVQIKEMFGPVQAAVAKAVADCVEEGLFGKS
                     DLESLVIMASIYLDPSAADYNKIYRYNYGATKLALSRAIEKFPDQKTLIYEKDRAAHA
                     VMGFKVQRLWDPPYLQVALDLVDMNKVHSVLAELPQNDHLIIEAGTPLIKRFGLSIIP
                     EIRKVRPNAFIIADMKILDTGNLEARMAADASADAVVISGLAPLPTLEKAIAEAKKTG
                     IYSVVDMLNVSEPLKVIKALKVKPDIVELHRAIDAESSEHAWGDIPALKKAAGGKLLV
                     ATAGGIRVDGVKKAFAAGADIIVVGRAITASKDVHTAADLFLEQMNKEEIDQFRVMT"
BASE COUNT         4104 a         6309 c         5838 g         4028 t
ORIGIN      
        1 tgctccttcc tggtgaggtt gcaccctccc gcaccgcaca cctcgaggct caccgtgtag
       61 gtgccgggct gcgaataccg atgcacggga gaagattcaa aggaagtcgt attgtcaccg
      121 aattcccaga gcgtgcctga tatatcgccc acagacaggt cgtcgaaccg gaccgcgaag
      181 ggtgcatacc cctcggtccg gttcgctgca aaatcggcca tgggggccgg gaacggtgag
      241 aattcccccc acaacgtgtg gtttgcagtc actccttcga accggaccgg tgatttggca
      301 tcataccaaa ctccgtcgat ctgcaatgcg tccagccggt acccgggatc ggcatggaag
      361 acgaactcgg ccgacccccc tctcccgact tcgacacacc cggaaggata cacggaacca
      421 tgggccccgg cccccgcgat gatccaacac ttcgcaggcc gctccatcac gaaggccggg
      481 aggtggagga cctgccctgc cggtacctca tactccccct ccaggccaaa atatccctca
      541 gccaaaaccg acaggtgatg gattccctgg tcaagtgcca ggcagagagg ggtcactccg
      601 caggcctggc cgtcaacgaa gacctgtgcc ccccgtggat cggaatcaac gcagagtgtc
      661 ccggtcgtta cccctgcgtc cgtatccggt tcttcgatcc cgctccctgc ctcctgagga
      721 ccatcagggg atgcggcggg tgagtccgtc gggacacatg ggttctcctt tataagggag
      781 atcgggtcca gagtgggcag gggtgtcatc ggaccacctg cctgggggag gaggggggat
      841 gaggacggat gggcagtgct ctcctgattc ccggtatctt ccaacggctc ccgcaccacg
      901 agagtgccgg cttttcccgg aagggtatac ggcgatccca cctcgtccct cattccaatc
      961 acctccacat cgatgccgca ggtcccggga gtgtctcccc ggatggtcag cgtggcgagc
     1021 accacctcgc agctccccgg gccaaccttc ctcccggtat cagaaccttt caggataacc
     1081 gaatcggatg ggaccttgct cttgctgttc agacctgccc atgacggaaa ccgtaccgcg
     1141 gtgatctcac ccgttgcagg gtccccgaga gtcaccttca cctcaaactc ggaaaggcct
     1201 ggtgaaaccc tgtcaagcac aagtatgcaa tcagcagtcc ccccaggcga ctcgatagta
     1261 cacggagtcg cgcgtatctc ggaccctgcc actatcccgc ataatgccag tgccacgagc
     1321 agcactactg cccctgcacg gccacccaaa ctacctgcct ccctccatgc tcccagcatg
     1381 caagctcctc cagcactccg ccgatctgat actcctcccc aagagccggt cacccggaga
     1441 tctgcgggat tctggtatat ttttcgataa tctctccggg tatttataat atcccccaaa
     1501 atgcgactga atacacgcag atcaccacca ctgccgggtg agatgccccc tcgcggcagg
     1561 tgttgcctct ctcccctggg gggtgcgctt gatgaaacct gcctggatga ggtagggttc
     1621 atggacctcc tcgatagtcc tcgtgtcctc gccgacggcg atggcaatcg tcttcacccc
     1681 cacggggccg ccgccaaact cctggacgat cacctggatg atacggcggt ccagctcgtc
     1741 gaggcccaag ctgtccaccc ccagcatgca gagcgcccgg tcggccacgt cacggtccac
     1801 cactccccct ccccgcacaa gggcatagtc ccccaccctc cggaggagcc ggttggcgat
     1861 tcgtggcgtg ccccggctcc tccttgcgat ctccatgctg ccgtcgggga ggatggggat
     1921 ccccatgatc gcggcgctcc gttcgatgat cctggcaagg tcctcgtggc catagaggga
     1981 gagccgggag atcaggccga accggtccct gaagggggaa ccgagcagcc cgatccgcgt
     2041 ggttgccccc acaagcgtga aaggttcgag tgtgatcttt atggaccgtg cgctcgggcc
     2101 ctcccctatg atcacgtcga tgaagaagtc ttccatcgcg gggtacagaa tctcctctat
     2161 caccgtgtgc aggcggtgga tctcgtcgat gaagaggacg tctccccgct tcaggagggt
     2221 gaggattgcc gcgaggtcgc cgggtttttc gagcaccggc ccgctggtgc agtgaataga
     2281 ggagcccatc tcccgggcga tgatgtgcgc aagggtggtc ttcccaaggc ccggaggacc
     2341 tgaaaagagg gtgtggtcca gtggttctcc acgcctcctg gccgcctcga tcgcaatgcg
     2401 gagggtctct ttcaaggctt cctggccgat aaagtccgaa aagcggtccg ggcggattgc
     2461 cgggtcatcg acctcgcctt cttccggcac gggcgagacc acgcgctcat ccatcccggg
     2521 tcaccgctcc cgcagcctgc gaagggatgc cttgatcacg tcctgaacgg tcgggcagtc
     2581 catcccggcg agcgtctccg agaccgcatc tcttgagtcc cggggggaga aaccaagtgc
     2641 aaggagggca ctctcggcgt cgctccttgt gggatcagcc ccggtatcac gtccctcgac
     2701 gagcagcgct gccttcttct tcatccggtc cttcaactcg aggatgatcc gtgcggcatt
     2761 cttctgccct acgccagata cccgggtcag ggccttctcg tcccctccta tcacggcggc
     2821 cgcaaggtcc gagactgtca tctgcgagag gatggcagtc gcgagcgcgg ggccgacccg
     2881 gttgacagtg atcagcagcc ggaacatctc gagctccccc ggtgcgagga agccatacag
     2941 cgccatcgta tcgtccctcg cgaccaggtg ggtgtagatc accacctcgg caggggacct
     3001 tgctgcttca tgtgccaggg gccgcggcat gaatacctgg aaccccaccc ctccggtctc
     3061 caggatcacg aacccgtcgc ctcccgcgac gacgcggccg cgaagcctcg caatcatcgg
     3121 gtattcctca cggtatggac atggcagagg gcgatcgaga ggccatcggc gacgtcgtca
     3181 ggcctcggga tgtccgggag accgaggagg cgcatgatca tctcctgcac ctgcttcttg
     3241 tcggccttcc ccgacccggt caccgcctgc ttgacctggt ttggggtata ctccacgacc
     3301 ggtatcccgc gtttccttgc ggcgagcatg atcacgcccc tcacctccgc cacaccaagt
     3361 gccgaggtga cgttgcggga gaagaagagt ttctccatgg ccatgcaccc ggggctatac
     3421 tcatcgagga ttgcggatat cgcgtcaaat atcctctcca ggcgctcagg agtccctccc
     3481 actgcagggg ccgtgcggat gcaggaatac gtgagcggga ccgtcctccc gtcatgtcgt
     3541 gccagaaccc catatcccac ggtggcaagc cccgggtcaa tgccaatcac tctcatggta
     3601 tcataaaatg gccgaaaatc actgcgattt attcattcta tcggggaatt taccagttat
     3661 acctttctat cgcggggtga aagagcgtaa ccgctcaacg gcgggagcca gaagaggcaa
     3721 tctccctgat atgcgggcaa aaccccccgg cctcgagtgc ctcccactcc ttcttcagga
     3781 ggagataata ctcatccctc gccttacgcc ggtcttttaa gacgaagacc atcccctcat
     3841 cgagcaccgg gtccggggtc ccggcaggtt tccgtccttt tccaagcgcc tcctgcagat
     3901 ccgcgatcca cacgtcgcag tcatggaggt tcccgagtat ctcctggagc ctcttcaact
     3961 gcctgatggc gggccggaac tccttcctgt acaggggcgc atagacctca aggaggtacc
     4021 gaagtctctt tgccgcgatg cgcatcgcgt ggtgcgcctc cacagccgag gggtcctgga
     4081 ccatcgggga gtatgattca agcttctccg cccgccgttt gatcgcgctt ccggatctct
     4141 tcatgggaaa cccgccgggt ggactctccc ggaggatgct gccgggcccg ctccaggcgg
     4201 caatccccgc ctccagaagt tcgattaccc ccttctgctc gagcctcgtg caatccgcaa
     4261 cgaccttcgg ctgtaatgcc tccctccttg tcctcttcat cgagaggagt atcgtgcacc
     4321 catccaccgc cggtccgcca tcatccctgt gttcctcgat cagtgcctcg aggtacccga
     4381 tctgcacgtc aaggtcacgg gcatccccga gatccctcgt gatgcgccgg atacccccag
     4441 agatcttctt gtaggccttc ccggggaagc actgcgagaa gagcgggagg cccgcccgca
     4501 acctccggga tgtcaccctc atccggtgga ctgcctcgac atccctccct tctttcacca
     4561 cttcgatctc ttcgcggaat tcgagggcga cccgggagag gaaccatgcc ccgtaacggc
     4621 agacctgcgc tctgccatca cctgatccct ttggcatgct gccagcaccc ccggtgtgca
     4681 atgagccatt tctgggcatt gcagggttct tctccctcct ttcccggcat atgggcatag
     4741 ttgccgtcca cgagcagctc catggtcttt tggttgtccc gcaggtgcac agggaggatg
     4801 acatccgcga tcgcccggtg aagttccggg tccttcacag ggaagagcac ctctacccgc
     4861 ctgtcgaggt ttcgcggcat caggtctgcg ctcccgagaa ggatctcctc ctttcccccg
     4921 ttacggaagt aatagatgcg tgcatgctcg aggaaccgcc ccacaatgct cgtgacggag
     4981 atattgtcgc tcgtgccggg tatccccggg cgaaggcagc agataccgcg tacctggagg
     5041 atgaccctga cacccgcgcg ggaggcccgg tacagggcca tgatgcattc gcgatcaacg
     5101 agcgcgttca tcttgaagat aatgcacccg tccccgtgct cctggtgccg cgcgatctcc
     5161 cgcccgatcc gggacaggat cccgctcctg atggattgcg gggctacgag gagtgtccgg
     5221 tacgaaggga tgctggcgta ccctgtgaga ctgttgaaca ggtccgatac atcctccccg
     5281 aggacaggat ctgccgagaa gactccgatg tctgtgtaga tgcggctgct ggaggcattg
     5341 taattgccgg tcccgaggtg cacgtacctg acgatgccgt ctttctcctt cctgattacc
     5401 atgcatacct ttgcatgcac cttgagaccc acgacaccgt acaccacgtg aacccccgcg
     5461 tgctcgagcg ccctcgccca cccgatattg ttctcctcgt cgaacctcgc cttgagttcg
     5521 atcagcgcgg ccactgcctt gccccgctcc cgtgcctcca ggagcgcttt taccaccggg
     5581 gactgtgcgc ccacccggta gagcgtcatc ttgatggcca gcacctgggg gtcgcgcgcg
     5641 gcctggcgta gaaacgctat cacaggggag aaactctcgt agggatgaaa gagcaggacg
     5701 tcctttctcc tgatgctgcg aaagatctcg tgctccctct caagcgcagg cggaacggat
     5761 ggggcgaacg gcgggtcctt caggtcgggc cggtccaggg agcagagctg catcaggtcg
     5821 gccatgccga gcggagcctc ctgggaatag aacatgtgct gcggcacccc cagtttacgg
     5881 ctgaggatcg cgcaggtctt tcgggggatg cccctgacga cctcgattcg taccggcgag
     5941 cctgtccggc gcatctccac cccttcctcg accgccgtca gcaggtccga tgcttcgtcc
     6001 tcctcgatct ccatgtccgc atcgcgtgtc accctgaacg ggtacgcggc tactacatcg
     6061 agcccgggga agaggaggcc caggttggcg ctgataagat cctcgaggaa gacgaaccgg
     6121 gcctcctcgc gaagactgct gtcccggtcg cgtccgggga tgcggaccag gcgggggaag
     6181 aggtgggtgg ggaccttcac tcttgcgaac cgatcctccc cggtcctcgg atcgtccacc
     6241 tgcaccgcga gggatatgga gaggttggag atgaagggga acgggtggga gcggtcgaat
     6301 gccagcgggg tgagcaccgg gaagatctca tgcctgaagt actccccgag gtattccctg
     6361 tcctccttcg gcagctcccc gtaccggtat acaaaaattc caaacgatgc cagggcaggg
     6421 aggagatcct ccttccataa cgccatctgc tccccgatga gcgggacaag ggcctggtgg
     6481 atggcctgga gctgccggga cgggctcatc ccgtccggtg gagattcgag gactccctgc
     6541 tccacctggc ggtgcagccc ggagacccgg atcatgaaga actcgtcgag gttattggca
     6601 aatatcgaga ggaacttgac ccgctccagg agcgggtggt actggaacgc cgcctccctg
     6661 agcactctcc ggttgaactc gatccaggag agttccctgt tgatgaagag tcccgggtct
     6721 tcaaggagat cgcaacgctc cctgccccgg gctgctgtcc ctttcactgg agaaggcccg
     6781 ctcatgttct tctacctatc aaggctactc tatcactctg gaatgccggg tacataagca
     6841 tgctgccgaa aggaggcaat tccaggggga ttatccgggg gattggagat cttcataccc
     6901 atttcaaaac tgttttacca taccagtgca caactaacac ttgctgtgat tcgccatggg
     6961 atccaatgaa gacatcatgc atgattttgt acgaagagag ctcaagaggc tttacccaag
     7021 cgtggatggc tggcagatca aaaacgtccc gaagtccggt gccgactcag ggttcatcat
     7081 atcccggagg ctgctcggtc gcttcgaagg ttcgcatgtc ctggtcagct tcgacaggag
     7141 cgtgacctcc gggaccgtca acgcgctgaa ggaaatggct gcatcgaacc ctgtgtcggg
     7201 ggtttcttcg ccgcgcctga tcctgatggc cccgcagggt gcggaggtcg caggcgttcc
     7261 gcccgaggtc caggtcctcc ctatgcagag tttcggctac gaggggaagg aactggtctg
     7321 gctgaagcgc agggcccagg taagcgagaa ggtcgcagca aaatccgctt aatccttcaa
     7381 tatattctct tttattcttt tttccaggtc ttttccgtga gagccctgcc agtagagctt
     7441 cctgcaccgc gggcaccaga agaactccat gcccggtgtg cttcgtgggg cgtggcgggc
     7501 cctcgaaacc tcatcttccc tggcagcacg aaggagggta ttgcagagcg agcaccggtt
     7561 cagcctgagt gcaggcacga tcaacccggt cctcgtgagc acccgcaact gctccatgac
     7621 atcccggtct tcaaggagga ttgcaccctc tccgcccctg cgtgcaagct cgcggtccat
     7681 cgtgagcagc accctccctt ctccttgggc cctcttcagg agcaatgtgt cctcgcgggt
     7741 atttccttcg cggtaccccg atgcgctctc agtgtcatag cccatgaagc gcaggtaccg
     7801 gcagagggtg ccgagcatcc ggtccgcgag aaaccgggtc ctccgcgttg gatcatccgg
     7861 ggttcccgac aaactcaatg cgttcaccgc accttgtgca catattcccc tcgaggttcc
     7921 ggaagactgc ggaaaatccc ctgcgctcga tgcagagcgc cccgcaccgg gggcagtagg
     7981 tgctctcgag agggtgggga tagacatttc caaggtaggg ataccggagc ccgagatccc
     8041 ttgcctgccg gtggatccgt tcaaggaccg aaacaggcgt ggaattcctg tcctgcatgt
     8101 ggtaatcggg gtggaagcgg gtgaagtgaa caggggtatt ctcgccaagg tgctcgatga
     8161 tccacctgat gagggcgccc gtctcttcct ggctgtcgtt caaccccggg atgacgaggg
     8221 tgacgacctc gacgtgcatc ccgagttccc tcgccacgac cgcagagtcg agcaccggct
     8281 ggaggcgggc tccgcatact ttcttgtaga actcctccga gaacgacttg atgtccacgc
     8341 gataggctgt cagcatggag gagagttcac ggagcgcctc ctcggtgatg tacccgttgg
     8401 tcacgtacac ggtgaaaagc ccttcttttc ttgcaagagt ccccatgtcg agtgcatatt
     8461 cgtgccagat agtgggctcg ttgtaggtcc atgagatact ggcgcacccg gccgccttcg
     8521 ccctgcggac cccttccccg ggtgagagat ccgggaggtg cgatgcgtcc cgctctgcgc
     8581 gggatatgtg ccagttctgg cagtgctcgc aacggaaatt gcagccaatg ctcccaaggg
     8641 agtacgagag ggtgccggga aggaagtggt aaagcggctt cttttcgatg ggatcaaccg
     8701 cttctgcact cactttccca taggtcgtgg catacaggac cccaccctgg ttcctcctga
     8761 caccgcagat cccggtcttc tcctccctga tggtgcagcg gtggctgcag agcgagcact
     8821 gcaccgcacc atcctctccc ggtgaccact ggagcgccct gtgcatgttc tcaatcaggc
     8881 gtgcaggaat tattaaggta atgcaccggg tctcctacaa gtgcgggtcc tccccttctt
     8941 ccccattttt agggcgctcg cactccataa tcagagaccc acggtacccg caggacttgc
     9001 accggtagat ctggccgatg tatcccccga ccacgcaata gacatccctg ctcccgcagg
     9061 acggacatcg cagcatatct tcaacactta tatgtgccgc atggagaaat gatagtgtag
     9121 catgacacgc gtggtcctct ccctcggagg ctcaattctc tttcccaccc tcgaggcgca
     9181 taccctccgc gcctgggcac ccgtgctggc gaggctcgca cagtcggtag acctcttcgt
     9241 ggtcgtgggg gggggcgggg aagcccggcg gtatatcagg gcggccaggg acacctgggc
     9301 agacgaggct ttctgtgacg agattgggat cctggtcacc cggctgaatg cgacacttct
     9361 gatcggtgca ctcggggccg tggcctatcc ccgcgtggcc ctcactcccg gcgaggcaat
     9421 ggaatacgca ggacaaggaa agatcgtcgt gatgggaggg gtcacacccg cgcagacaac
     9481 cgatgcggtc gccgcagtcc tcgcggagcg ggcgagggct gccctccttg tcaacctcac
     9541 ttcggtagac ggcatctatt ccgccgaccc gaaagaacac ccggatgcac gccgctactc
     9601 ctccatgaca ccaaaggagc tggtagagat tgtggggtgc gggggccttg cagccggatc
     9661 gaacacggtg gtggacatgg tggccgcaaa agtggtggag cggagccgga tcccgctcct
     9721 cgttcttgac ggccgcgatc ccgcgctcct cgagaagtcc ctcttctccc acgagtgcgc
     9781 ggggacaata gtcatcggcg accaggagaa cccgctcccc cttgagaagg gacgctcacc
     9841 ataacctatt tgccaagccc tgatcaatac ctgatctcgt tcggggtagt agggtagcct
     9901 ggtccatcct agagcgtttg ggacgctttg acggcagttc gaatctgccc taccccatga
     9961 gcatggacag ggaaacggcg tgcaggatat actccttact tatgaaaaag tacgatgacc
    10021 ccaggtctcc ccgcacgttc ctcgagttcg agaatcccta ccagatcctg gtcctcacca
    10081 tcttatccgc ccagaccacc gaccggaacg tgaatgccat ccgtaacgag atttttgaac
    10141 ggttcccgga gccagaagac ctcgccaaag cccgcacaga agaggtcgag gcaatcatcc
    10201 ggagcctcgg gttctaccat gccaaggcct cacatatcat tggcaccgca cggtcgcttg
    10261 tcgggaggtt tggcggcaga gtgccccgaa ccatggagga actcctcacc ctccccggtg
    10321 tcggcagaaa gacggcaaat atcgtgctga accatgcgta tgggatcgac gaagggattg
    10381 ccgtggacac ccacgtccgg agggtatcag gccgcattgg gttctcttcc cacccggacc
    10441 cctcccggat cgagcacgat ctcatgcgcc ttttcccagt ggaggtctgg ggaaacctca
    10501 actacctcct tatcaggcat gggcgggctg tctgcaccgc ccggaacccg aagtgcagcg
    10561 aatgccctgt acgcccggac tgtgcctatt attccctgca ctccaactgc gtggaacccc
    10621 ggggatcaat cagaccccga agagcgcacg gtagaccaga aaccctgcaa acgcacagct
    10681 cgcggtgagc gggatcgtca ggacccaggc agccacaatc cggcggacga ttccccactg
    10741 cactgccgag taccccctgg tggctcccac ccccatgatc gagccgctta ttgcatgggt
    10801 ggtggagacc gggacgccga acgccgtgac aaaggagagc accactcctc cacccgtctc
    10861 cgcacagaaa ccctggtaag gacggagctt ggtgatcttc tttcccatcg tttccactac
    10921 cctccagccc cctaggaatg tgccgagcgc gatggcaagg cctgaaatga tgattaccca
    10981 tgccgggacg acaaactcgg agaggagccc cgcggccacc aggagcgcgg tgatgactcc
    11041 cattgcattc tgggcatcat tgctcccatg ccccatactg taaaaggcag cagagaagac
    11101 ctggagtttc gagaagataa aattcatcca cttggagttc gctttcctga acagccggat
    11161 gatcgccatc ccgaggagat aagcggcgat catcccaagg gtgggggaga cgactatgaa
    11221 gatcacgatg gccaggatgc cgcttacggg gaggatcccc gtaatcactc ccgccggaat
    11281 cacgatggat atgccgcaca gtgccccaag agcggtatac tcgggccagt tctcctcccc
    11341 cttccttctc gcggtcatgg agaggacaac agccccgcag atgccgccca ccgcggccat
    11401 caccaccgcc ctcaagaaga gatccattga aggccagaga accgacccga tacccccgaa
    11461 cgcgattgca gacccgataa gccctccgac aagcgcgtgg cttgccgata tgggaatgcc
    11521 tgcaacagac gataaaaaaa cccacaggac cgccgcgcac atccccacca ggatgacctc
    11581 cgtagtgaga aaaccggact ccacgattcc ctgtccaatc gtctttgcaa ccaccgtggt
    11641 aaaaatgaaa ggccccgcca tgttgaagaa tgccgccatc agcacggcct ggagggggga
    11701 aagaaccctg gtggctacca cggtggcgat ggaatttgcc gcgtcattga gaccgttgac
    11761 aaaattgaag aggagtgcaa gaaatatccc ggcgatgacg atattctcca tgttcactca
    11821 tgaatgccgt atcgcgatgt ccgagagcac atttgcgaca tcctcgcact tgtcggtcgc
    11881 gatctccagg tactcgtaga tgtccttgcg cttgatgata gcaatcgcat cattcgacct
    11941 gaagaggtcg tcgtaggcgt ggtgaaggac gtcgtcggca aggttctcca gccggttcac
    12001 ctcgatgcac cgctgttcta tctggcgggg gttcttcatt gacctgatcc ccttgactgc
    12061 ctcctccagc tcgacaacgg acaactggat cagtttcgca agctccacca tatgcggatc
    12121 cgttcccgga atcccgtaat gatacatcct ggtggcggtg ctcttgatgt aatcgaggat
    12181 gtcatcgagc gccgaggcaa gctttgagat ctcttccggg tcaagaggag tgatgaaggt
    12241 ctggttcagc tgttcgtaga tctcgtgggt gatcttgtcg ccctcgtgtt cgagctgctc
    12301 gatctggtgg actttctccc ggacagaggt gtagtcgtcc acaaggtcca cgatgcggta
    12361 ggaggcaagg accaccaccg ctgccagcct ctcgaagagg gtgaagaaca cctcgtcctg
    12421 cgggattatc cattctttga tgcccaaagc gtatcacaag gagatttcag agcagcaggg
    12481 aaaaacactc cggtttgatg atcccaagaa tgagatggac cgagcgggaa ttgaacccgc
    12541 ggcctcttcc atgccaagga agcgatcttc cgctgatcta tcggcccgtg cctacaatgt
    12601 atgatgcagg ggcttaaaag aattgtttcc cgccactggg agaaaaaccg caattcattg
    12661 ggttttcctg cgcccttgac ctttcaaccg attcacgcgc ggcgcgaggg atctcccatg
    12721 actccctccc taaagacagg gatgcgtgcc aggacacgtg ctcttgcctc ctcgatcgtc
    12781 gagcggatga ggactgctcc cctgcggatc accggggtca taaggggcac tgctccgggg
    12841 ggggcggccg cttccacggg cagcgtcacg tgccttcccc caggatattc ccagacctgc
    12901 ttggcaccgc tccgcttccc cctcttggcg catttctttc cttccttctc caccatgtcc
    12961 atggcaaaat cgatcacagg ggcgttggcg atcgctcctc ccacaccgaa tgccccgaca
    13021 atatcccgga acctggccac gtcctcgagt gtgacgcctc cggagaggaa gatcgccact
    13081 tcggggaaac cagcggcatc gagttcccag cggacctctt caaggattgc ccgcatgtcg
    13141 cccctccgcg atcgtggggt gtcgagcctg accgctgccg cgccgcagcg ggcaccggtg
    13201 agagactccc tcttctcatc gcagaaggtg tcgcagagca tgatgcggga gacttccggc
    13261 ggggaatggc gatcgaacgc ctggaatgcc tcggccggcg atgaatagca cattacgaat
    13321 gcatgcggca tggttcctgc aagaggaatt ccagcggggg cacaggtgtt gctcaccccg
    13381 tcgacgccgc caatccacgc cgagcgctcg accatcgtgg cgatagcggg gtgctgccgg
    13441 cgtgacccga aggagaacac catccgttcc gctgcaactg ccttgatcct ggcggcggcg
    13501 gtggcgattc ctgatgcatg gcagaggaac ccgaggattg ccgtttcgta ccgggcaata
    13561 tcgcggtact tcccttcgac gcgaagcacc ggctcccatg gatgaaagag cgagccttcc
    13621 ggcatggcat acgcatcaag ggggagatct tcaaggaggg atagcacgtc ttccatcccg
    13681 cagaggatgc ccatgccccc agggaccgcg gacgccgtga cctccatggc aacgattgga
    13741 ttgttgcccg aagcagagag gaccgcttcg ctccttgaga aatagatgtc cgtgcactct
    13801 ccctttttta tccgttcgtc atcgacaatg gcaaacctgc ccatggatgt attgtgggag
    13861 cggtgaaaag atacagatat tgactccccg cgcagagtcg caccgaaagc cagaacaacc
    13921 gcccgttcca gattacttaa ggacaagatc atgaaccgca cgattttcac ggcggtgacc
    13981 tgatgctcgg gatcatcggg ggcacaagcc ttctctttgc gaggcttccc gacctggaga
    14041 agagggtggt gcacacgccg tatggcaaca gcgaggtgat ggcaggagaa gtcgccctcc
    14101 tcctccggca ccagcgggac atcccgccgc accggatcaa ctaccggtcg cacctctcgg
    14161 cccttgcgct cctcggcgtg gaccgcctgg tggtgctcgg gtcgtcggga tcgctcaaaa
    14221 aagagatccc gccggggagc atggtgatcc cgaccgatta cttctcctct gcagccgtcc
    14281 caacccttca cgaccatgcc atatgccacg tgcaccctgg gttctctccg gagttgtcaa
    14341 accatctgaa ggagattgtc cccgaggcaa ttttcggggg gacttacgtc cagacaaggg
    14401 gcccgaggat cgagacggtg gccgaggtca gggcgcttgc cctctgcggc gatatcgtgg
    14461 gaatgacgct tgcaagcgag gcgacccttg caatcgaact cgggatagag gttgcggcgc
    14521 tctgcaccgt cgacaactac gcaaacggcc tctcggacga actgctcact tacgagagga
    14581 tcgtggaata ctcggcgcgg tataaagatc ggacggagga gattatctcc cgcatcatcc
    14641 gggaactcgc ctaggagatt acatcatgaa gaatacgcaa acaaaaggaa cttcgctcct
    14701 gatcgagcgg gtcttggtga acggaacgct ccaggatatc ttcgtggacg agactggggt
    14761 tatcacttcg ataggggagg gaatcgcacg cacctttcgc ggtgaagcgg agacctgcat
    14821 cgacggtgcc ggcgacctgg cgttgtcggg cctcgtgaac acccacaccc acgccgccat
    14881 gaccctgctc cggggctttg ccgacgacat gccgctgcag gattggctga cccagaagat
    14941 ctggcctctc gaggcccacc ttaccgggga ggacgtatac tggggcacga aactcgcgtg
    15001 ccttgagatg atcaggtccg gcaccgtggc cttcaatgac atgtacttct tcatggatga
    15061 ggccgcccgg gcggttgacg agatggggct ctgtgcggtc ctcgcctatg gattcctcga
    15121 cctcttctcg gaggagaagc gcgaggccga gtgccgggcg accgagcgcc tggtgcaatc
    15181 gatacacggc atgaaaaatc ccaggatcaa agcagccgtc gggccgcacg caatgtacac
    15241 cgtctcgccc gaaggactga ggtgggtctc ggaattcgct gccgaaaagg agatcgggat
    15301 ccacgtccac ttaagcgaga cggaaaagga ggtaacagat gcggtctccc agtggcagaa
    15361 gcgcccggtg aagatccttg atgaatgtgg catcctgaca ccccggacgg tggccgccca
    15421 ctgctgctgg cttgatgggg acgagtgcag cctccttgca aaacgggggg tgcacatctc
    15481 ccacaacccg gccagcaaca tgaagcttgc tacagggagg gccatgccct atcattggct
    15541 gaaggaggca ggggcatccg tcaccctcgg gactgacggg tgcgcctcta acaacaacct
    15601 ggacctcttc gaggagatga agatcgcatc gctcctccag aagcattcct ggaactccca
    15661 gacactgctt cccgccgggg aagcgctcgc gatggcgacc tctgcaggcg caaaggctct
    15721 tgggttcgga cggggcgtga tcgctcccgg agagcctgcn nnnnnnnnnn nnnnnnnnnn
    15781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15841 nnnnnnnnnn nnnnnnnnna ctccctgatg agtaaagcgg catccctctc gatgacaatg
    15901 ctccatcggg actgatgcag cgtaggcaca tcccctgaac atgattgaga cagtgctggg
    15961 aaacaggaag taccgattgg tatctcggta aaccctgccc acgggctatc gcgtatggag
    16021 gaagggggat tctcctcctc ccacctatgt gaaaaccgca ctcctcacat gatgacgctc
    16081 tcggggcttc gccccgccac tgtggcatcc cctgagtgcg atagagccag tgctgggtat
    16141 ctcgaagaat cagaggtttc cagtatgctc ttcttgcaga ctatcccgta tttggggggg
    16201 gtcggggagg gggattctcc ccctccctgt caaagaacct agcgaactca atttcccacc
    16261 tcagtcaaac cgcactcacc acacgatgac gctcctgggg ctgatacccc cctatatttg
    16321 cgcaccctcc tgatcacgat agggccagtt cctggagtgc aatttcggcg aaatccatca
    16381 cgttcaaccc tgcccgcagg ccatcgcgta aactggagag tgtgcgggaa gaggtgaaat
    16441 cccttctcca ctgtttcacc tgctgccgga taagaagaaa acagtaaatc tcagcggctg
    16501 ccataccacg tcggcggaag aggaacaagg accccgctct tcatttaccc tcacgccgtc
    16561 tcgatcttcg aggcgtcttc gagcccgagt gccttgatcg aggcctcgcg catcttgaac
    16621 ttctggatct tcccggtgat gctcatcggg aactcgttga cgaacatgac gtaacgcggg
    16681 attttgtagt gggcaatctt cccgcggcag aactccttga cctcctcttc tgaaagagag
    16741 tgcccgttct ccaccatgac ccaggcgcag agctcctccc catacttgac atccgggacg
    16801 ccgacgacgt acacgtctgc gatcttcggg tggtggtgca ggaactcctc gatctctctc
    16861 gggtagatgt tctcgccgcc acggatgacc atgtccttta agcggcctac gatcttcaca
    16921 tacccttcct cgtcgatagt cccgaggtcc ccggtgtggt tccagtggtt ctcgtcgagc
    16981 gtggcattag tggcgctcgg gttgtggtaa tagcacttca tcacgcaata gccgcgtgcg
    17041 catatctcgc cgatctcgcc ccggggcacg atcttcccgc tttttgggtc gatgatcttg
    17101 atctcggtgt gaggaaagac ccgtcccacc gtcgagaccc ggcgttccag cgggtccttt
    17161 gtcgtggtca tcgtcacgcc gggggcggtc tcggtctgcc cgtagacaat gacgatctcg
    17221 gacatgttca tcttccggtt gacctccttc ataacctcta tcgggcaggg ggatccggcc
    17281 atgattccgg tgcgaagcgt cttcatcgag tacttcggga agtcggggtg cgagagctcg
    17341 gcgatgaaca tcgtgggtac cccgtgcagg gcggtgcagc gctctttctc gacggtcttt
    17401 agcacctcca gggcatcgaa tgtgggagac ggcaggacca tcgtgcttcc gtgggtcatg
    17461 caggcgatgt tcgagaggac catcccgaag cagtgataga acgggactgg gatgcagagc
    17521 cggtccttct cggaaaagtt catcccctcc ccgataatgt agccgttgtt gaggatgctg
    17581 tggtgagaaa ggacgacccc cttgggaaag cccgtggtcc cgcttgtata catgatattg
    17641 atgggatcat cgaagtcaag cgactcctcc cgggccacga gctcatcctt gctcaccgcc
    17701 tggccgcggg agatgatctg cgaccaggag aacatcccct gctgcggttc atcgcccatg
    17761 aagatgacat tcttcaggaa tgggaacttc tcggtctcca ggtgccccgg gcgcgaggtg
    17821 tcgacttcag ggcatgcctc gcggaacatc ccaatatagt cagaggtctt gaaccggccc
    17881 tggacaatca ctgtctggat ctcggcctgc ttgaggacat actcgagctc gtacgtgcgg
    17941 taggccgggt tgatattcac catgatggct ccgatcctgg cggtggcgca ctgggtcagt
    18001 acccactcgg catggttcat tgcccagatc cctacgcgat ctcccttctc caccccgatc
    18061 gccataaggc ccctggcgag atcgtttacc ctcccgagga attcaccgta ggtgtagcgg
    18121 atcccctggt gcaccgagac cagcgcatcg gtgcggggat acttccgggc tatccggttc
    18181 agcatctcac ctatcgtgta cccgagcagc ggataggtag aggtcccgca atcatagctg
    18241 cctgcatcca tcctaaagag gtgagtcacg agatcttata tccccaccgg atcaacagca
    18301 taaccattaa atcggcacaa agcgacctta tagagtcacg gggtcgtggc ctagtccgga
    18361 atggcgacgg gctccagcgg tctttcgcgt cgtgtggatg atgaacaatg ggtctcctga
    18421 tgaaaagatg atgatccgtt ggagcactga cgcatgtcgg agagacccgt cgatcgtgag
    18481 ttcaaatctc accgacccca cattttctgc acatccatct tcgccacgga taactccgtc
    18541 cgtaaaccat ggacagggga gaacgtttca tcttatctac gacataacac tcacctggaa
    18601 tgcggtatga cgaatgacag gttagaacga ggaagagcgc agttgaacac aatgcatggt
    18661 gcgggtgtcg attcgatgct cgacaacctg gcagaaatcg ctcccgacct cggcaggttc
    18721 acagtcgagt tcccctacgg agatatcctc tgcaggcccg gccttgacat aagatcgcga
    18781 cagattgcaa cggtcgccgc cctcactgcg ctcggcaacg caccggtcca gctgaacgcc
    18841 catatctcca tggcgttgaa cgtcgggtgc acgagggaag agattgtcga ggtcatcacc
    18901 cagatggcag tctatgctgg gtttcctgcc gcactgaatg gcatggcggt agcaaaggaa
    18961 gtcttttcag caagagaccg ggaaaagacg aagtgaaatg aggacgagtc ggatcacgtc
    19021 tcctgcagag tttttggacc attccatcat tttcggattt tcgcagcacg ggaacgtctt
    19081 caatccggct gcccgatctc cggcatgaaa gggaaccggt agcgcccctc cataccccag
    19141 gattccgacc attacaaggt ttatcatata atgcagccac atattacaca ggagtcgtgg
    19201 caattttatg taccttatag gtgaagcact ggtgggcgac gggccggaac tggcccatgt
    19261 cgacctgatt cttggggaca aggaggggcc ggtaggggcc gcatttgcaa acgccctctc
    19321 ccagctctct gccgggcata cgccgctcct cgcggtggtc cgcccgaacc tcctcgcaaa
    19381 gccggcaacc ctgatcgtcc ccaaggtgac gctgcggaag ggggtccaga tcaaggagat
    19441 gttcggtcct gtccaggccg cggtggcaaa agccgtcgcc gactgcgtgg aggaggggct
    19501 ctttgggaaa tcggatctcg agtcccttgt catcatggca agcatttacc tcgatccctc
    19561 ggccgctgac tacaataaga tctaccggta caactacggg gccacgaaac tcgcgctttc
    19621 gagggcgatt gagaagttcc ctgaccagaa gacccttatt tacgagaagg accgggctgc
    19681 ccacgcagtc atggggttca aggtccagcg gctctgggac ccgccatacc tgcaggtcgc
    19741 ccttgacctc gtggacatga acaaggtgca ctccgtcctc gcagagctcc cgcagaacga
    19801 ccacctgatc atcgaggccg ggacgcccct gatcaagcgg ttcggcctct ctatcatacc
    19861 ggagatccgg aaagtcaggc caaatgcctt catcatcgcc gacatgaaga tcctcgacac
    19921 cgggaacctc gaagcccgca tggccgccga tgcctccgcg gatgccgtgg taatctcggg
    19981 cctcgcccct ctccccaccc tcgagaaggc gatcgcggag gcgaagaaga ccggaatcta
    20041 ctcggtggtt gacatgctca atgtctccga gcccctgaag gtaataaaag cgctcaaggt
    20101 caagcccgac atcgtcgaac tccaccgggc gatcgatgcc gagagctcgg aacacgcatg
    20161 gggcgacatt cccgccttga aaaaggctgc cggaggaaaa ctgctggtcg caaccgccgg
    20221 aggcatccgg gtagacggcg tgaagaaggc gtttgccgca ggtgcggaca tcattgtggt
    20281 cggccgggca atcacggcaa gcaaggacgt ccatactgcg gcggatttat tcctggaaca
    20341 gatgaacaag gaagagatcg accagttccg ggtgatgac
//
LOCUS       DAOQYR010000033        20129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_3034_length_20129_cov_9.105211, whole genome shotgun sequence.
ACCESSION   DAOQYR010000033 DAOQYR010000000
VERSION     DAOQYR010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 20129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20129
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3034_length_20129_cov_9.105211"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PKM87_05615"
     rRNA            complement(<1..330)
                     /locus_tag="PKM87_05615"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01959"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01959"
     gene            complement(900..1640)
                     /locus_tag="PKM87_05620"
     CDS             complement(900..1640)
                     /locus_tag="PKM87_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HNQ29580.1"
                     /translation="MPEEPVEILVQMAERGEIDPWNIDIVEVTDRFLSELERRKELDL
                     RISGRTLFYAALLLRMKSEILEQAEVVEDDEYCGEEDVGEEEFGPDAEFDQEGNALGP
                     IERLEREIRRRLDRKQLRKRPITLFELILELKAAEKEQRRRQRFISPFDPRLIDVEDV
                     VGIAHDEGYRETAMAVLCQCEESIEKHGIMTLEELAEMMGKRIIDVYIPLLYLMFEGK
                     LALWQDEFFGQVFVQVWRAEDVPAAIEN"
     gene            complement(1633..5073)
                     /gene="smc"
                     /locus_tag="PKM87_05625"
     CDS             complement(1633..5073)
                     /gene="smc"
                     /locus_tag="PKM87_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNQ29581.1"
                     /translation="MHITELEIDNFKSFTKKTKIPFYEGFSVISGPNGSGKSNIIDSI
                     LFVLALSGSRTLRAEKLTDLINLNSGRHTAEVTLAFSDGTRIRRRIKRTSSGYYSYIY
                     LNNRLSKQVEVTDFLAKHGIKPHGYNVVMQGDITRIIEMSDFERRKIIDEIAGVAEFD
                     QKKGQALIELEVVRERIEREELLLRELSQRLEELAAEREQALKFRHWQERLQHLEGLR
                     AAATLHAREKDLAALSRVIEDERLQLDRVVSDRSIEENEREYLKKDLADIDSQINQKS
                     GAEYLRLIAELEEAKGTIKLAEQTISRLKKEKEAGLEGINRTYIDQKRAETRVSECTQ
                     AIREMNIDRTNLAMEAATAAAQKEKIEQQIQSQSRDAEGARDELFALMQSIEEKKNER
                     SSLLHQKDMLIEKSRMRTSERERMEERLKALEAEGAGKEQRLSENEREMEALTNEKAE
                     LDRALSELESSVFARRTTLERVREEYRSHEQDVVRLEAQQQARGEAGSRVLDAVLGMD
                     GVLGTIAQLGKAPPEYATALNVAAGQRLHYVVAKDDGVAARAIEYLKEQKMGRVTFLP
                     LSRLKPPDLPPVRDEGVIGYAVQMLEFQPQYADAFRVVFGGTVVVESISRGRRLLGKY
                     RMVTPDGELLEKSGAMTGGSFKKPIRGFGAAVEDEISRARSQMEGLAAEIADIERAVK
                     RGGEEIDAKRARRSELDAAISRLAVISEEFSRQEEVTQREKTQINEVLLALAEEVRSG
                     TAELAALEAALDSITDAIGQAQKKIELTKKKLEDTSIPVLSEQLEKKKRECEDIERRL
                     RNKEADINDLQRERQHFSTRLDELKAEVERIQAKNEEIDREVAQSNSQIETSRARIGE
                     IEERQKQFSSELLELRNRHTQVSDTIRESGQKILEFDAAAERHRIQVSALTERFTALS
                     AEIEALRMEAGETETDLSLQEIEDGMAEAAQQIKRLGAVNMLAIEEYERVEGRIAERG
                     EKKEVLSRERATLLERIEKFEKMKYEAFMTAFKAIDANFREVFARLTSGSGHLILENE
                     EDPFSGGLSFAVQPRDKPVHLLSALSGGEKSLTTLAFIFSIQQHIPAPFYAFDEVDMS
                     LDGSNVERIATMIKELSGTSQFILVSLRKPMIEGADRILGVTLLPDKSSFVTGIKSHA
                     "
     gene            complement(5088..5651)
                     /locus_tag="PKM87_05630"
     CDS             complement(5088..5651)
                     /locus_tag="PKM87_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29582.1"
                     /translation="MIINEGSPECHPPPHHRYERRTSPEESDMEIIKDKDRSLAPVDE
                     RVAALEKKMREMEAMVKGLTEELLDLKSVAMKLSKASEERSRAEMRMSKPAAPAASSP
                     IILQKKSVSAPAMQRPAQAPVETAPEKMDMIMQPNGTLAPEKRKNSDYIIASAGFSKK
                     KQSAAGPGKKNDLIVAEDEETDSAHKK"
     gene            5886..7124
                     /gene="glyA"
                     /locus_tag="PKM87_05635"
     CDS             5886..7124
                     /gene="glyA"
                     /locus_tag="PKM87_05635"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNQ29583.1"
                     /translation="MSNLATTDPEIADIVRKEMERQRDGLELIASENMVSRAVLEAMG
                     SVMTNKYAEGYPGKRYYGGCEFYDMAENLARDRLKELFGAEHANVQPHSGTQANMAVY
                     FATMKLGDLMLSMKLSQGGHLSHGSPVSFTGQFYRVAQYGVDPKTEILDYGVVEEMAR
                     KERPAMLVCGASAYPRTIDFRAFQEIADGVDAYCMADIAHIAGLVAAGLHPTSVGVTD
                     FTTTTTHKTLRGPRGGAIMCNSEYAQIVDKAVFPGMQGGPLMHTITAKAVCFKEALRP
                     SFKEYNRQIIRNSKALAETLHEKNLRLVSGGTDNHLILLDLSDLGITGLEAEVALGRA
                     HITVNKNTIPNEQRSPFVTSGLRVGTPAVTSRGMKEEEMRQIGEWIGTILRDIHNEGA
                     IRRIGEEVVSMAGKFPLYPE"
     gene            7126..7968
                     /gene="folD"
                     /locus_tag="PKM87_05640"
     CDS             7126..7968
                     /gene="folD"
                     /locus_tag="PKM87_05640"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNQ29584.1"
                     /translation="MILDGKKVSEARLARLKEEVEASGLTPELATVIVGKDPASQMYV
                     RMKHRACGQVGIRSRGVELPSDSTTEEVREQVDRLNQDQDVSGILVQLPLPPHVDTHR
                     VVETVLPEKDVDGFHPANLGRLFSGNPIFSPCTPRGIMTLLQEYGVEPEGLRAVVIGR
                     SIEVGRPMAALLLNASATVTICHSRTRELPSFTRQADILVAAVGKARFVEPTMVREGA
                     VVIDVGTNQVDGKLCGDVDFDRVREIASAITPVPGGVGPMTIASLMENTFLAARLQSC
                     TGAR"
     gene            7950..8762
                     /gene="folP"
                     /locus_tag="PKM87_05645"
     CDS             7950..8762
                     /gene="folP"
                     /locus_tag="PKM87_05645"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNQ29585.1"
                     /translation="MHRCTIGGLTVGDGSPVRLMGVINCSPESFYSESYTPAGDVFSK
                     ACQLVEQGADIIDVGARSTAPGAPPLTRGEEAARMMQALSVLEGSGIRVSVDTMDPAV
                     LEACLEYDIAAINDISGLLNPEYAAGVSGSGLPALLMASKWAVGDACSLDETHRALSM
                     VETRAHASGVTEYVLDPAIGLWQASRTADLDWELCRQFRQFSVYQRPLLAAVSRKSFL
                     GNLVGKPPAGRLAASIAMASLLVCMGADIVRTHDVAETRDAILVAEKMRRWQ"
     gene            8759..9565
                     /gene="cofE"
                     /locus_tag="PKM87_05650"
     CDS             8759..9565
                     /gene="cofE"
                     /locus_tag="PKM87_05650"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HNQ29586.1"
                     /translation="MNSSFTTIGIRTPLIREGDEIASILVRAIRETPAREIQDGDILV
                     VAETAISTAEGRVVRLESVVPDRDAQRLAHRYGMDPALVQCILDESDEVVGGIPGFLL
                     CMKHGTLLPNAGVDASNAPPGCVTPLPADPDASALRIKEVIELLTGVRVGVIVADSRT
                     HAMRVGCSGVAIGCAGIPSVMDARGRTDLFGRPLMVTRLALADNLASAAEVVMGEADE
                     RTPAAVIRGLGIQVTDISGIESINAEECIFMGLLKQERKRQSSSSHRNDT"
     gene            9802..10356
                     /locus_tag="PKM87_05655"
     CDS             9802..10356
                     /locus_tag="PKM87_05655"
                     /inference="COORDINATES: protein motif:HMM:NF015827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HNQ29587.1"
                     /translation="MRIQEIFAILLIAAGIFIAAAAADVNDQSSSEQVSLIGMANPSA
                     VWAEQMGYEYLIRTNPDGSQYGVCVLPDGSECDAWELFRQSLAVEQEVSLIGLPDPSA
                     VWASQRGYEYLIRTNPDGSQYGVCVLPDGSEHDSWRTFREFCGISKPAGMSMIQPASP
                     RAILSPKETFGEAHFSARLAALTP"
     gene            10534..10800
                     /locus_tag="PKM87_05660"
     CDS             10534..10800
                     /locus_tag="PKM87_05660"
                     /inference="COORDINATES: protein motif:HMM:NF015827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HNQ29588.1"
                     /translation="MRQLALLLIVIIILAVALFCGCTTREEAPSSHGGQQTIGNPASL
                     YCHSLGYHTEIRTDSNGSQYGVCCMPNGTEVDEWVLYRQGHPEA"
     gene            10866..11447
                     /locus_tag="PKM87_05665"
     CDS             10866..11447
                     /locus_tag="PKM87_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ29589.1"
                     /translation="MTEQVIILEPGEERAQHIARALSSRTAGKVLDILSDGPRQLSFI
                     SDQLDIPLPQVKYHVDNLLDAGILEVIDERYSVKGRRVKIYGLKHQVLVVAPKMVNLK
                     ALLLKYSSVCGLFLLAALAFSLFHDKIFSTETPLLLGGGSIGPAPQPTVMSILLDHIP
                     TDPLTILLFCAGGGVAVGASLILELYFLRKGER"
     gene            complement(11571..12206)
                     /gene="cofC"
                     /locus_tag="PKM87_05670"
     CDS             complement(11571..12206)
                     /gene="cofC"
                     /locus_tag="PKM87_05670"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HNQ29590.1"
                     /translation="MPVVALIPFKPVNPKTRLSCVLEPGERESFARCMLADVIEAVSG
                     AGCEPRIISTEPYACRGATVEVIPGGLNETLNALLALAGAPVLIIMADIPLATPGAVS
                     RLISTGRDMAIAPGRGGGTNAIYLSEGSSYRVDYYGASFLKHARIARERGLSCEVIDS
                     FRLHTDVDEKEDLVELLIHGRGRSREYLESLGFTISIEKGRVGVVRKGVHG"
     gene            12328..13326
                     /gene="cofG"
                     /locus_tag="PKM87_05675"
     CDS             12328..13326
                     /gene="cofG"
                     /locus_tag="PKM87_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase subunit CofG"
                     /protein_id="HNQ29591.1"
                     /translation="MPQRTITYSRNVFLPLTTVCHNRCGYCSFRTPVHEDCVMSPGQV
                     EDILQRGAALGCTEALFTFGERPGLEPGFSRILGKMGYRDIIDYCYDLCEKAIGIGIL
                     PHTNAGILTMEEMDRLSEVNASMGLMLETTARVDAHRNSPGKVPAVRIGMIEDAGKLR
                     IPFTTGILLGIGEGMDDREESFMVIRDLHKRFGHIQEVIIQNFCPKDKTPMAGCSPVE
                     PEEICLTIRLARDILPGEIAIQIPPNLADAASLIPCGVDDLGGVSPLTIDYVNPEHPW
                     PEIRELKRLAGDARLKERLCIYQRYIDKGWCPPRLAPLISRLNQMIQHRSDRSAEG"
     gene            13313..14026
                     /locus_tag="PKM87_05680"
     CDS             13313..14026
                     /locus_tag="PKM87_05680"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNQ29592.1"
                     /translation="MQKGDLLYAGKAKSVFLTDREGILLVEFRDDITAFDGGKKDILD
                     KKGCFNAKVSAYIFRLLTENGIPTHFIDMVDDRRMLVRSLSMIPLEVIVRNVAAGSMS
                     RTYPIAEGTPLSPPVIVIDYKDDSRHDPMLNDDLILALKLLSAQELARVKDLALAVNS
                     VLVRFFESLGITLVDFKIEFGKAGEDIMLGDEISMDSMRLWDMHTGESLDKDVYRFSK
                     GDVMEVYRRVAEKICAGGK"
     gene            14029..14304
                     /locus_tag="PKM87_05685"
     CDS             14029..14304
                     /locus_tag="PKM87_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HNQ29593.1"
                     /translation="MAEDELESEILEWAKKYAQEHTWVLNPDTKKLDIVVRGLARNQR
                     KFGERYCPCRLRSGDPEKDRDIICPCVFHRDEVERDGSCHCNLYFRK"
     gene            complement(14389..16044)
                     /locus_tag="PKM87_05690"
     CDS             complement(14389..16044)
                     /locus_tag="PKM87_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNQ29594.1"
                     /translation="MVTRFRRYWQIADVLFKYGFEIFVQRLFPGVHRFRLRKSSPVDS
                     AASEYQRMRMALEELGPTYVKFGQILSTRQDMLPPGLIEELKRLQDKTNPLPFETIMG
                     VLENACPGYREYFSEIEEIPVASASLSQVHRARLHDGTPVALKVQRPGIEEIIETDIL
                     ILESAARRGERSFPEWKVYNPRGIVRDFAVQIRKELDFLRDGKNADLLRVHMRDLEGV
                     KIPRIYWKYSSRHLLVMEYIEGVRVDNVEAIRAYGHSPKRIAEAGFFAYMKQIFEDGF
                     FHGDPHPGNLIVTKEGELVFLDFGIIGIIRPERRFWFVQLLNSMVLLDAGMLMKSLEG
                     LSVKIPEESRELLRDEIYTAMLEAEGISIGQFNFTAMTNAFTTILREYRIQVPGNLML
                     MLKVIIMVLDVGVTLNPEFRFKDQAESYMARFRKSESIVDQLRSRAGGSMLEAMDGFL
                     DMPRNINQMLKQLSTGIIRIDVVDTDIRRLQMSLDRTSNKVLIGLVVSGMVVGSSFIL
                     RETQLPVPGVVFYIAILAYVIALCIGFYAIYKVLSTGVDEKER"
     gene            complement(16107..17795)
                     /locus_tag="PKM87_05695"
     CDS             complement(16107..17795)
                     /locus_tag="PKM87_05695"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNQ29595.1"
                     /translation="MEDPLRRALLLFALQNAVKHQSIPKAGTVIGMVMGKYPELKPRA
                     REATTLIKEVIDEVTALTPAERADRLCSLAPELVEEMSRPREHAKALPTLEKAENGVV
                     MRFAPNPSGPLHLGHARAAVLNDAYVRRYGGRYVLRIEDTDPKRVDPHAYAMVKEDIE
                     WLGLAISDIVYQSDRFDIYYRYGRELIERGGAYVCTCDNERFKEMKLARKACPCRGRG
                     IGEDLVLFEKMRAGEYGEGEVTLRVRTDLDHPDPAMRDYPAFRILKSPPHPRMDVCMY
                     PLMNFSVAIDDHLLGITHVIRGKDHIANTRRQRYIFDYFGWEPPVYRHYGRMGIEGVV
                     LSTSQMRAGIAAGEYRGWDDIRLGTLRAIARRGISPEAVREAVLDIGIGETDISFSWE
                     NLFARNKAIVDPSSNRYFFVPDPVPVRVQGGPDHVAHALLHPAHPERGTRDLHFTGEV
                     ILPREDLAEGRAMVRLKDLFNIEMTGEGTDISACFAGDSLADARARKAPIIQWLPAGA
                     GVPCTLVTPDGDRAGMCEPRVAAELGQVVQFERVGFARIDAVSAEGIIAYFTHR"
     gene            complement(17797..18759)
                     /locus_tag="PKM87_05700"
     CDS             complement(17797..18759)
                     /locus_tag="PKM87_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNQ29596.1"
                     /translation="MELNSYLEQTANQVDSLIHRYFGSSCDDLGRASAHLLLAGGKRL
                     RPALLLLSADAVNRGSSVDVVPAALALELTHSFTLIHDDIMDGDLVRRGVPTVHTQWD
                     EPTAILAGDVLFASAFEFISLADAADNAKVRATSMLARTCVEICEGQHMDMAFEKRDD
                     VSEGEYMKMVEKKTGALYAAAAGIGSILAGGNPNFTDALYHFGQGIGMAFQIQDDLID
                     LLASPEVSGKDQASDIREGKKTLIHIKALEKGFDLSQFQRRLSQAEIQEIITRLRGIG
                     VISEVQDTARNLVKTGQKRISILPESEERDLLIHMGDHFISRSY"
     gene            complement(18781..20118)
                     /locus_tag="PKM87_05705"
     CDS             complement(18781..20118)
                     /locus_tag="PKM87_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase J family beta-CASP ribonuclease"
                     /protein_id="HNQ29597.1"
                     /translation="MDIEIIAVGGYDEVGRNMTAVRCGKDIVIFDMGLRLDQVMIHEE
                     AEVENMHSLDLIQMKAIPDDTMMNTVEGSVKAIVCSHGHLDHIGAIPKLAHRYNAPII
                     STPYTTELIRQQIAGEQKFGVNNKLFALRAGQRYTLSQQLTLEFVRMQHSIIDTVTAV
                     LHTPKGAIVYACDYKLDRTPVIGEPPDFARLRQIGKEGVLALIVEATYIDNRGRAPSE
                     RIARDLVRDTITSYEDDKSAIIVSTFSSHIARVKTIAECAHEIGRKPVLLGRSMEKYS
                     STAEQMKLVSYPDTMSMFGNRRTVDRTLRRMMKAGKEKFVPIITGHQGEPGSILTRIA
                     LGDTPYKVEKGDKVIFSAKVIPNPMNVGQRYMVEAHLNMAGARIFPDLHVTGHAYRED
                     HYEFIHLLNPQHIIPAHGHIRMTSGYAEFTEAIGYTLHNDVHILSNGQRVKMT"
BASE COUNT         4087 a         6051 c         5481 g         4510 t
ORIGIN      
        1 tgcgccccgt agggcctgga ttcgtgtctc agaatccatc tccgggctct tgctctcaca
       61 acccgtaccg atcaatggct tgttgggccg ttaccccaac aacaacctaa tcggccgcag
      121 acccatcctc aggcggcgga cctttgaatc agggggcatt ccagcgtccc tgatctatgg
      181 ggtattatcc ccagtttccc ggggttatcc ccctcctgag ggtaggttat ccacgtgtta
      241 ctgagcagtg cgccgaggtc ttgcacctct cgactcgcat ggcttaatcg aaccccgata
      301 gcagtggcct ctggcaggat caaccagaat tctcgataaa tccagcacac tatcgttcgc
      361 ttacttcgag gaattagcgg ttaccaaaca aatttccgca ttgccagtac caacgtcaga
      421 accaatgaca gaactattgt ccggtcgtgt ttggttctcc atcacagagg gtaacaatat
      481 aggtgttgtt gctatttaac cttttttgat gtcggttttt caccggcgat aaaggcctgc
      541 aaccacccgc cctgccgggc ggatacaccc cggaacagca caatccgcat ccccgctgcc
      601 cgcaaaggca tggagacgat tttcctgttt ccggtctcaa tcctcagaac cggaacatgc
      661 acccgttgtc cggttcttca ctgtgatatt gagggaatac atgttgaaca cagttgtata
      721 tgaaggtttt ccatgatggg cgatactccc ccggccattg catatctcct gatatgaaaa
      781 aaattgcaat ttaagtccaa aaatttaaaa aaggagttct caacccttta aatcgccatc
      841 gagacagata ataacgaatt gccattcgta tttctctgat tacgcctcta atatccattt
      901 taattctcta ttgcagcagg aacatcttct gcgcgccaga cctggacgaa gacctggccg
      961 aagaactcgt cctgccagag cgcaagcttc ccctcgaaca tgaggtagag gagagggata
     1021 tacacatcga tgatacgctt tcccatcatc tccgcaagtt cctccagcgt catgatgccg
     1081 tgcttctcga tgctctcctc gcactggcag agcaccgcca ttgcggtctc gcggtatccc
     1141 tcgtcgtgcg cgatacccac cacgtcctcc acgtcgatga ggcgggggtc gaacgggctg
     1201 atgaaacgct gtcggcgcct ctgctccttc tccgcggctt tgagttcgag gatcagttca
     1261 aagagcgtga tggggcgctt cctcagctgc ttcctgtcaa gccggcgacg tatctcgcgt
     1321 tcaagccgct cgatggggcc aagcgcgttc ccctcctggt caaattctgc atcgggtccg
     1381 aactcctcct cgccgacatc ttcttccccg caatactcgt catcttcaac gacttctgcc
     1441 tgctcaagga tctcggactt catgcggagc aggagcgccg cgtagaagag cgttcttccc
     1501 gaaatcctca aatcaagttc cttcctcctc tcaagctcgg agaggaaccg gtccgtcacc
     1561 tcgactatat cgatattcca gggatcaatc tccccgcgct ccgccatctg caccaggatc
     1621 tctacgggtt cttcaggcat gcgacttgat cccggtcaca aacgagctct tgtccgggag
     1681 gagcgtgact ccgaggatgc ggtccgcgcc ctcgatcatg ggttttcgga gggataccag
     1741 gatgaactgg gacgtgccgg acagttcctt gatcatggtc gcaatccgtt ccacgttcga
     1801 cccgtcaagg gacatatcca cctcgtcgaa cgcatagaac ggagccggga tgtgctgctg
     1861 gatcgagaag atgaaggcaa gcgtggtcag agacttttca cctccggaga gggcagagag
     1921 gaggtgaacc ggcttgtccc gcggctggac ggcaaacgag agccctcccg agaacggatc
     1981 ctcctcgttc tcgaggatga ggtgcccgct cccgctggtc aggcgcgcaa agacttccct
     2041 gaaattcgca tcgatcgcct tgaacgcggt catgaacgcc tcgtacttca tcttctcgaa
     2101 cttttcgata cgttcgagca gtgttgcccg ctcccgggag agcacctctt tcttctcccc
     2161 gcgttcggcg atcctcccct ccacgcgttc atattcctcg atggcaagca tgttcactgc
     2221 tccaagcctc ttgatctgct gggcggcctc ggccatcccg tcttcgatct cctggagcga
     2281 gagatcggtc tcggtttcac cggcctccat cctcagcgcc tctatctccg ccgaaagggc
     2341 ggtgaaacgt tcggtgaggg ccgaaacctg gatcctatga cgctcagcag cggcatcgaa
     2401 ctccaggatc ttctggcccg actcccttat agtgtctgat acctgggtgt gcctgttccg
     2461 aagttcaagg agttctgaag agaactgctt ctgccgttcc tcgatctcgc cgatccgggc
     2521 ccggcttgtc tctatttgcg agttcgactg cgccacctcc cggtcgatct cctcgttctt
     2581 ggcctggatc ctctccactt cggccttcag ttcgtcaaga cgggtcgaaa agtgctgcct
     2641 ctcccgctgg aggtcattga tgtcggcctc cttgttccgc aggcggcgct cgatgtcctc
     2701 gcactccctc ttcttcttct cgagctgctc cgacaatacc gggatggagg tatcctcgag
     2761 cttcttcttg gtcaactcga tcttcttctg ggcctggccg atggcatcgg tgatcgagtc
     2821 aagagccgcc tccagtgccg caagctccgc tgtgccggag cgcacctcct ccgcgagcgc
     2881 caaaaggacc tcgtttatct gcgttttctc cctttgtgtc acctcttcct ggcgggagaa
     2941 ctcttcgctt atcaccgcga gccgcgagat cgctgcatcg agctccgatc tcctcgccct
     3001 ctttgcatcg atctcctcgc cgcccctctt gacagcccgt tcaatatcgg caatctctgc
     3061 tgccagtcct tccatctggg acctggcacg ggatatctcg tcctccactg ccgcaccgaa
     3121 accgcggatc ggtttcttga acgaaccgcc ggtcattgcc ccgctctttt cgagaagctc
     3181 tccgtcagga gtgaccatac ggtacttccc gagcagcctc cgcccgcggg agatcgactc
     3241 cacgacgaca gtccccccga acaccacccg gaatgcatcc gcgtactgcg gctggaactc
     3301 aagcatctgc accgcatacc cgatcacccc ctcgtccctg acagggggaa gatcaggtgg
     3361 cttgagacgg gagagaggga ggaacgtcac cctccccatc ttctgctcct tcaggtactc
     3421 gatggcccgt gcggcaacgc catcgtcttt tgccaccacg tagtgcagcc tctgccctgc
     3481 cgcaacgttg agggcagtcg catattcggg cggagctttt ccaagctgcg cgatggtccc
     3541 taataccccg tccatcccga gcaccgcgtc gagcacgcgg cttcccgcct caccgcgggc
     3601 ctgctgctgt gcctccagcc gaactacatc ctgttcgtgg gaccgatact cctccctgac
     3661 cctctcgagg gtggtacggc gggcaaacac gctgctctcc agttccgaga gggcacggtc
     3721 aagttccgcc ttctcattgg tgagggcttc catctcgcgt tcgttctcgg aaagccgctg
     3781 ctccttccct gctccctcgg cttcaagcgc cttcagccgc tcctccatcc tctccctctc
     3841 cgatgtgcgc atccggctct tctcgatgag catgtccttc tggtggagaa gcgaggagcg
     3901 ttcgttcttc ttctcctcga tgctctgcat cagggcgaag agctcgtccc tggccccctc
     3961 cgcatccctg ctctgcgact ggatctgctg ctcaatcttt tctttctgcg cagcagccgt
     4021 cgctgcctcc atggcaaggt tcgtgcggtc gatgttcatc tcccggattg cctgagtgca
     4081 ttcggagacc cgtgtctcgg cccgtttctg gtctatatag gtcctgttga tcccctcaag
     4141 cccggcctcc ttctccttct tcaacctgga aatggtctgc tccgcgagct ttatcgtccc
     4201 cttggcctcc tcgagctccg cgatcaggcg cagatattct gccccgctct tctggttgat
     4261 ctgggaatcg atatctgcaa ggtccttctt taggtattcg cgctcgttct cctcgatgct
     4321 acggtcggag acgacccgat cgagctgcag ccgttcatcc tcgatcaccc tggagagtgc
     4381 ggcaaggtcc ttctccctcg cgtgcagggt tgcagctgcc ctgagcccct ccaggtgctg
     4441 gagccgctcc tgccagtgcc ggaatttcaa tgcctgttcc cgttctgccg caagctcctc
     4501 aaggcgctgc gagagctccc gaagcaggag ctcctcgcgt tctatccgtt ccctcaccac
     4561 ctcgagctcg ataagcgcct ggcccttttt ctggtcgaat tctgccactc ctgcaatctc
     4621 atcaatgatc ttgcggcgct cgaagtcact catctcgatg atccgggtga tatcgccctg
     4681 catcaccacg ttgtacccgt gcggcttgat gccgtgcttt gcgaggaaat ctgtcacctc
     4741 gacctgcttg gagagccggt tattgaggta aatataactg taatacccgc ttgacgtgcg
     4801 ctttatacgc cggcgtattc tggtcccgtc cgagaacgcg agtgtgacct ccgcggtatg
     4861 gcggcccgag ttcaggttta tcaggtctgt cagcttctcg gcacggagag tccgcgaccc
     4921 cgaaagggca aggacaaaaa ggatactgtc gatgatgttg ctctttcctg atccatttgg
     4981 ccctgatatg acagaaaaac cctcgtaaaa cgggatcttg gtcttttttg tgaaagattt
     5041 gaaattatca atctcaagct cggtgatatg caaagaccaa ggccccgtta tttcttgtgc
     5101 gcagaatccg tctcctcatc ttcggccacg atgaggtcgt tcttttttcc agggcccgcg
     5161 gcactctgct tctttttgga gaaaccggca gatgcaatga tgtaatcact gttcttccgc
     5221 ttctcgggcg caagagtccc gttcggctgc ataatcatgt ccatcttctc gggagccgtc
     5281 tctaccggtg cctgcgccgg ccgctgcatc gcaggtgcag agacggattt cttctggagg
     5341 attatggggc tgcttgcggc gggtgctgcc ggcttgctca tgcgcatctc cgcccgcgac
     5401 cgctcttcac tcgccttcga gagcttcatg gccacggatt tgaggtccag cagctcctcg
     5461 gtgagcccct tgaccatggc ctccatctcc ctcatcttct tctccagggc cgcgacccgt
     5521 tcgtcaacgg gagcaaggct ccggtccttg tcttttatta tctccatatc tgactcctcg
     5581 gggctggtcc gcctctcata cctgtggtgg ggcggtgggt ggcattcagg agagccctca
     5641 ttgattatca tgaattattc ccctataata attatgctgc cagttgcggc ggtttttttc
     5701 aaatttctct cgcttaatcc cactctcatg gagttgcaac cgaacacccc ggtattccgg
     5761 aacgtttttt taaaccctcg atccggcgca ttttccagtc taactgtgag aagaattgag
     5821 atccttgagt ttttttttca ctggagaaga aaaggtatta cctgatcaga tcaacctttt
     5881 tatacatgtc caatctggcc accacggatc cggaaatagc agatatagtc aggaaagaga
     5941 tggaacgcca gcgtgacggc ctggagttga ttgcctccga gaacatggtc agccgtgcag
     6001 tgctcgaggc catgggctct gtcatgacca acaagtatgc ggagggttac ccggggaaac
     6061 ggtactatgg agggtgcgag ttctacgaca tggcagagaa cctcgcacgg gaccggctca
     6121 aggaactttt cggggcagag catgcgaacg tgcagccaca ctcaggcacc caggcaaaca
     6181 tggcggtcta ttttgccacc atgaaactcg gggacctcat gctctccatg aaactctccc
     6241 aggggggcca tctctcccat gggtcacctg tcagcttcac gggtcagttc taccgggttg
     6301 cccagtatgg agtagacccc aaaacagaga tactggacta cggggtggta gaggagatgg
     6361 cgcggaagga gcgcccggcc atgctggtgt gcggggccag cgcatacccg agaaccatcg
     6421 atttccgggc attccaggag atcgcggatg gagtagatgc ctactgcatg gccgatatcg
     6481 cccatattgc agggcttgtc gccgccgggc tccacccgac atctgtcggg gtgacagatt
     6541 ttaccactac aaccacccat aaaacgctcc gtggaccccg cgggggcgct atcatgtgca
     6601 attccgagta tgcccagata gtggacaagg cggtcttccc gggtatgcag ggcggccccc
     6661 tgatgcatac catcaccgca aaagccgtct gcttcaagga ggcgctccgg ccctctttta
     6721 aggaatataa ccggcagatt atcaggaact caaaagccct cgccgaaacg ctccatgaga
     6781 agaacctccg gttggtttca ggcggtaccg acaatcacct catcctcctt gacttgagcg
     6841 acctcggcat caccggcctt gaagctgagg ttgcgctcgg gcgggcacat atcacggtga
     6901 acaagaacac cattccgaac gagcagcgca gccccttcgt gacgagcggc ctccgggttg
     6961 gcactcctgc cgtgaccagc aggggcatga aggaggaaga gatgcgtcag atcggggaat
     7021 ggatcgggac aatcctccgt gacatccaca acgagggggc tataaggagg atcggggagg
     7081 aggttgtgtc catggccgga aagttccccc tctacccgga gtgacatgat cctcgatggg
     7141 aagaaggtgt ccgaggcacg acttgcacgg ttgaaggaag aggttgaagc atcgggcctt
     7201 actcccgaac tcgccacagt aatcgtaggc aaagacccgg catcgcagat gtatgtccgc
     7261 atgaagcaca gggcctgcgg acaggtgggc atcaggtcaa gaggtgtcga actcccctcg
     7321 gactccacca ccgaggaggt acgcgagcag gtcgataggc tcaaccagga ccaggatgtc
     7381 agcgggatcc tggtgcagct tccgctcccc ccgcatgtcg acactcatcg cgtggtggag
     7441 acagtcctcc ccgaaaaaga cgtagacggt ttccaccccg caaacctcgg gcgtctcttc
     7501 tcagggaacc cgatcttctc gccctgcact ccgaggggga tcatgacact cctccaggaa
     7561 tacggggtcg agccggaagg gctgcgtgcc gtggttattg ggcggagtat agaggtggga
     7621 cgaccaatgg ccgccctcct tctcaatgcc agcgccacgg tgacgatctg ccacagccgg
     7681 acccgcgaac tcccttcatt cacacgccag gccgatatcc tggtggcggc agtgggaaaa
     7741 gcccgttttg tggaacccac gatggtcagg gagggagcgg tggtcattga tgtcggcacg
     7801 aaccaggtag acggaaaact gtgcggggat gtcgactttg accgggtcag ggaaatcgcc
     7861 agtgccatca ccccggtacc gggaggggta ggcccgatga cgattgcatc cctcatggag
     7921 aacaccttcc ttgcggcaag gctgcagtca tgcaccggtg cacgataggt gggctgactg
     7981 ttggtgacgg atcccccgtc cgcctgatgg gggtgatcaa ctgcagcccc gagtcattct
     8041 actcagagtc ctataccccg gcgggggatg tcttttcgaa ggcctgtcag cttgtcgaac
     8101 agggtgcaga tatcatcgat gtgggtgcga ggagcaccgc tcccggcgcc ccgccactca
     8161 cccgtggaga ggaggctgca aggatgatgc aggccctctc ggtgcttgag ggatcaggca
     8221 tccgggtctc ggtggacaca atggacccgg cggtcctcga ggcgtgcctc gaatacgata
     8281 ttgctgcaat aaacgatatc tcgggcctcc tcaaccccga atacgcggca ggggtgtctg
     8341 gatctggtct ccctgcactc ctgatggcat cgaaatgggc ggtcggcgac gcgtgcagcc
     8401 tggatgagac gcaccgggcc ctctcgatgg tggagaccag ggcgcacgca tccggcgtga
     8461 ccgagtacgt cctcgatccg gcaatcggcc tctggcaggc cagccgcacg gcagatcttg
     8521 attgggaact ctgccggcag ttcaggcagt tctctgtata ccagcgccca ctgcttgccg
     8581 cagtttcgcg caagtccttt cttggaaacc ttgtaggaaa accacctgca ggcaggctcg
     8641 cggcatcaat cgccatggcc tcgcttctgg tctgcatggg tgcagatatc gtccgaacac
     8701 acgatgtcgc cgagacacgc gatgccatcc tcgtcgccga aaagatgagg cggtggcaat
     8761 gaattcttcc ttcaccacaa tcggcattcg cacccccctg ataagggaag gcgatgagat
     8821 cgcttcgata ctcgtccggg ccataagaga gactcctgca cgggagattc aggatggtga
     8881 tatcctggtt gtcgcggaga ccgcaatctc cactgcagaa ggacgtgttg tccgcctcga
     8941 atcggtagtc ccggacagag atgcccagcg cctggcccac cggtatggga tggatcctgc
     9001 cctcgtccag tgcatccttg acgagagcga tgaagtagtt ggtggaattc ccggatttct
     9061 cctctgcatg aagcacggga cgctccttcc gaacgcaggt gttgatgcct cgaatgcacc
     9121 gccaggctgt gtgacacccc tgcccgcaga tcccgatgcc agcgcattac gcataaagga
     9181 ggtgattgaa ctgctcaccg gggtccgcgt gggtgtgatt gtcgccgaca gccggacgca
     9241 tgccatgcgc gtggggtgca gtggggtcgc tatcggctgc gcggggatac cctctgtaat
     9301 ggacgcacgc ggccggactg accttttcgg caggccgctc atggtcaccc gcctggcact
     9361 cgcggacaac cttgcctccg ctgccgaggt cgtgatggga gaggcagacg agcgcactcc
     9421 ggccgccgtt atccggggtc ttgggatcca ggtgactgac atttctggta ttgagtcaat
     9481 taatgcagaa gagtgcatct tcatggggct gctgaagcag gagagaaaac ggcaatcctc
     9541 ctcttctcac cgcaacgata cctgaatact ggtggaatac tcttttcaag ggagatcctt
     9601 cctcttcttt ttttttcgcc accctataag tataaaaaaa tttgaactca aagaatgatt
     9661 aatggcctca aatagcaatt ctaaaagtgt tggggctgat tccacaacca gcagacctct
     9721 cgaatcccca ggcaaccgtc caaccagggc atggaggtct gctgcatgga atgggctctg
     9781 tccaacaaga gaacgatgaa tatgaggata caagagatct tcgccatatt gttgattgcg
     9841 gcaggtattt ttattgcagc cgcagccgcg gatgtgaatg accagtcgtc ttcagaacag
     9901 gtgagcctca ttggaatggc caatccttct gcagtatggg ccgagcagat gggatacgag
     9961 tatctcatca ggaccaatcc tgacggcagc cagtacgggg tgtgcgtcct ccctgacgga
    10021 tcggaatgcg atgcctggga gttattcagg cagtccctgg ctgtcgaaca ggaagtgtca
    10081 ctgattggac ttccggatcc ttccgcggta tgggccagtc agaggggata cgagtatctc
    10141 atcaggacca atcctgacgg cagccagtac ggggtgtgcg tcctccctga cggatcggaa
    10201 cacgattcat ggagaacatt ccgcgaattc tgtggaattt caaagccggc aggcatgtcc
    10261 atgatccagc ccgcatcccc cagggctatc ctgtccccga aggagacgtt tggagaggcc
    10321 catttcagtg cccgtctggc ggcacttaca ccctgaattt tataggtaga ggggatggac
    10381 ttgcgttccc gccaggtcgc cggaatggac ctcccccacc atttcacagg cattggtttt
    10441 tttcctgcta ccccctccag ggaacgaact tcttccaggg actgtgagaa ttgatatccc
    10501 tttgctctga aatgatatgt tgcaggtgaa cgcatgagac aactggccct tctcttaata
    10561 gtgataatta tactcgcggt ggcccttttc tgcggctgca ccaccaggga ggaggcgcca
    10621 tcctcacacg gggggcagca gacaatcgga aatcccgcct cgctctactg tcactcgctt
    10681 ggatatcata ccgagatacg caccgattcg aatggatccc agtatggggt ctgctgcatg
    10741 ccgaatggga ctgaagtcga cgagtgggta ctctataggc aaggtcatcc ggaagcgtaa
    10801 cgcaaggaaa tttcctcatc caaaaagaag gtgaacgcac caaggctggg atgtcggggt
    10861 aatgaatgac agaacaggta ataatccttg aacccggaga ggagagggct cagcacattg
    10921 ctcgtgcgtt gtcaagcagg actgccggaa aagtactcga tattctctct gatggtccaa
    10981 ggcaactttc attcatctcc gatcaacttg acatccccct tccccaggtg aaataccatg
    11041 ttgacaacct cctcgatgcg gggatcctgg aagtcataga tgagaggtac agcgtaaagg
    11101 ggcgccgcgt caagatttac gggttgaaac accaggtcct cgtagttgct cccaaaatgg
    11161 tgaaccttaa agcattgctt ttgaagtatt catcggtctg tgggctgttt ctgctggcag
    11221 cccttgcctt ttccctgttc catgacaaaa ttttctccac cgagactccg ctgctcctcg
    11281 gtggaggcag catcggaccg gctccacagc ccacggttat gtcgattctc ctggatcaca
    11341 ttcccactga cccgctgacc attctcctct tctgtgcggg aggcggcgtg gccgttggcg
    11401 cctcactgat acttgaattg tatttcctca gaaaaggaga gagataatct gcttcgcata
    11461 tgtcaatccg gatctgcgaa tctttcacat acttccgata agacgctccg gggttttccg
    11521 ggaagatttc ctgtgacttt taatgtctcc catgcgtagc caaggcgcat tcaaccatga
    11581 acaccttttc ttacgacccc taccctcccc ttttcaatgg agatggtaaa accgaggctc
    11641 tccaggtact ccctgctcct ccctcgccca tggataagga gctcgaccag gtcttccttc
    11701 tcgtcgacat ccgtgtgcag ccggaacgag tcgataacct cgcatgagag cccgcgctcc
    11761 cgggcaatcc gggcatgctt caggaaactg gcaccataat agtcaacacg gtaggaagat
    11821 ccttccgaga ggtatattgc attggttccc ccgcccctgc ccggcgcgat ggccatgtcg
    11881 cgcccggtgg agatgaggcg actcaccgca cccggagtcg cgagtgggat atctgccatg
    11941 atgataagga cgggggcgcc agccagtgca aggagcgcgt tcagggtctc gttcagtcca
    12001 ccggggatga cctcgaccgt tgcccccctg catgcgtagg gctccgtgga tatgatccgc
    12061 ggctcgcatc ccgcaccgct gaccgcctcg atcacgtccg cgagcataca cctcgcaaag
    12121 gactcccgct cccccggctc cagcacgcac gagaggcgtg tctttgggtt caccggcttg
    12181 aatggtataa gcgcaacgac cggcatgcct gcttcttttc cgtttgtgtc catgaccaca
    12241 aaatacctct tgatttggaa tgcgccgcac ccctgccaca tatggtccat tcattaggga
    12301 tgaaaccgat tgaacatggg acgagagatg ccacaaagga ccatcactta ctcgcgcaac
    12361 gtctttttac ctctcaccac cgtttgccac aaccggtgcg gctactgctc cttccgcacc
    12421 cctgtacacg aggattgcgt gatgtccccg ggacaggtgg aagacatcct gcagagaggt
    12481 gccgcacttg gatgcacgga agccctcttc accttcgggg agcggccagg cctcgaaccg
    12541 gggttttcca ggatactcgg aaagatgggg taccgggata tcatcgacta ttgttacgat
    12601 ctctgtgaga aggccatcgg catcgggata ctcccccata cgaatgccgg aatcctcaca
    12661 atggaggaga tggatcggct ctcggaagtg aatgcaagca tgggcctgat gctcgagacg
    12721 acagcccggg tggacgccca ccggaattcg cccggcaagg tccccgccgt ccgtatcggg
    12781 atgattgagg acgctgggaa gctgcggatc cccttcacca cgggcatcct cctgggaatt
    12841 ggggagggga tggatgatcg ggaagagtcg ttcatggtca tccgcgacct ccataaaagg
    12901 ttcggccaca tccaggaggt gatcatccag aacttctgcc cgaaggacaa aacccccatg
    12961 gcaggttgct cccccgtcga gcccgaggag atctgcctga ccatccggct cgcaagggat
    13021 atcctccctg gcgagatcgc catccagatc ccgccgaacc tcgcggatgc tgcatccctc
    13081 atcccctgcg gtgttgacga cctaggcggc gtctccccgc tcaccatcga ttacgtcaat
    13141 cccgaacatc cctggcccga gatcagggaa ctcaagcgcc ttgccggcga tgcccgtctc
    13201 aaggagcggc tgtgtatcta ccagcgctac atcgacaagg gatggtgccc gcctcgtctc
    13261 gcaccactta taagcagact gaaccaaatg atacagcata ggagcgatcg cagtgcagaa
    13321 gggtgacctg ctctacgcgg gcaaggcaaa atcggtcttc ctgaccgaca gggaagggat
    13381 cttactcgtg gagttccgcg atgacatcac cgcattcgat ggaggaaaga aggatatcct
    13441 cgacaagaaa ggctgtttca atgcaaaggt ctccgcctat atcttccgcc tgctgacgga
    13501 aaacgggatc ccgacccatt tcatcgacat ggtggacgac cgcaggatgc ttgtccggtc
    13561 cctctcgatg ataccactcg aggtgattgt ccggaatgtt gctgccggtt ccatgagccg
    13621 cacgtacccg atcgcagagg ggacgcccct ctccccgcct gtcatcgtga tcgattacaa
    13681 ggacgattcg cgccatgacc ccatgctcaa cgatgacctt atccttgccc tgaagctcct
    13741 cagtgcacag gaacttgcca gggtcaagga tctcgccctt gcagtcaatt ccgtgctggt
    13801 gcggttcttt gaatcgctcg ggataaccct tgtggatttc aagatcgaat tcggaaaagc
    13861 gggtgaagat atcatgctgg gtgatgagat cagcatggac tccatgcgcc tctgggacat
    13921 gcacacgggg gagtcactcg acaaggacgt gtaccggttc tcgaagggcg acgtgatgga
    13981 ggtgtacagg cgtgtggcgg agaagatatg tgcgggaggg aaataatcgt ggctgaagat
    14041 gaactggaga gcgagatact ggaatgggca aagaagtacg cacaggagca cacgtgggtc
    14101 ctcaaccctg acacgaaaaa actggacatc gtcgtccggg gacttgcccg aaaccagagg
    14161 aaattcggag agcgctactg cccttgccgc ctccgatccg gcgaccctga aaaagaccgg
    14221 gacatcatct gcccctgtgt cttccaccgg gacgaggtag agcgggacgg cagctgccat
    14281 tgcaacctgt atttccggaa gtaaggcggg tagggatcac gatcgttccc gggatgttgt
    14341 agtcccggcc ctttttctct gtgacatgta tttccggata gaataatttt agcgttcctt
    14401 ttcgtcaacg cctgtcgaaa gaaccttgta gatcgcatag aagcctatgc agagggcaat
    14461 gacataggcg aggatcgcga tgtaaaatac gacgcccggc accggcagtt gagtctcccg
    14521 gaggatgaac gacgaaccga ccaccatgcc cgagacaacg aggcctatca gcaccttgtt
    14581 gctcgtccga tccagcgaca tctgcaaccg ccggatatcg gtatcgacga catcaatcct
    14641 gatgatcccc gtcgagagct gcttcaacat ctggttgata ttccttggca tgtccaggaa
    14701 tccgtccatg gcctcgagca tggagccccc tgcccttgac ctgagctggt ccacgatcga
    14761 ctcgctcttt ctgaacctcg ccatgtacga ttcagcctgg tccttgaacc ggaactccgg
    14821 gttcagggtc actcccacat caaggaccat gatgatcacc ttcagcatca gcatgaggtt
    14881 ccccggcacc tggatgcggt actcgcggag gatggtggtg aatgcgttgg tcatcgccgt
    14941 gaaattgaat tgccctattg agatcccctc ggcctcgagc attgcagtat aaatctcgtc
    15001 gcggagtagc tcccttgact cctccgggat tttgactgat aggccttcca gcgacttcat
    15061 cagcatcccc gcatctaaaa ggaccattga attcaggagc tggacaaacc agaagcggcg
    15121 ttcaggccgg ataatcccaa tgataccaaa gtcaagaaaa acaagctcgc cctccttcgt
    15181 gacgatgaga ttgcccgggt ggggatcgcc gtggaagaac ccgtcctcga agatctgctt
    15241 catgtaggca aaaaatccgg cctctgcaat ccttttcggg ctgtgtccgt acgcccggat
    15301 agcctccacg ttatccaccc gcacaccctc gatgtactcc atcaccagca ggtgcctgct
    15361 gctgtatttc cagtagatcc tcgggatctt cacgccttca agatcgcgca tgtggacacg
    15421 cagaaggtca gcatttttcc catcgcgcaa aaaatccagc tctttcctga tctgcaccgc
    15481 aaaatccctt acgatccccc gggggttata caccttccac tctggaaagc tcctctctcc
    15541 tcgcctggca gcggactcga ggatcaggat atcggtctcg atgatctctt caataccagg
    15601 cctctgcact ttcagcgcga cgggggtgcc atcgtgcagc cgggccctgt gaacctggct
    15661 gagggatgcg gacgcaaccg gtatctcctc gatctcgctg aaatactccc tgtatccggg
    15721 gcaggcattc tcgagcactc ccatgatcgt ctcaaacggg agcgggttgg tcttgtcctg
    15781 caggcgcttg agttcctcaa tcagcccggg cggaagcata tcctgccggg tgctgagaat
    15841 ctggccaaat ttcacgtatg tgggcccgag ttcctcgagg gccatcctca tccgctgata
    15901 ttcgctggcg gcagaatcaa ccgggctgga tttgcggagt cggaacctgt gtaccccagg
    15961 aaagaggcgc tgaacgaaga tctcgaaccc gtacttgaag agtacatcgg cgatctgcca
    16021 gtagcgacgg aatctggtta ccatgtcatc atacctggcc agggggaagt gggagaaggc
    16081 caaaaagccc tccggtgagt gccagattaa cgatgggtga aatatgcgat aataccttcc
    16141 gcgcttaccg catcgatcct ggcaaatccc acccgctcga attgcaccac ctgacccaac
    16201 tcagccgcaa cgcggggttc gcacatcccg gcgcggtccc cgtcaggggt gaccagcgtg
    16261 caggggacgc ctgctcccgc cggaagccac tggataatgg gggccttccg ggcccgcgcg
    16321 tctgcaagcg agtcgccggc aaaacaggca gaaatatccg ttccctcacc agtcatctcg
    16381 atgttgaaga gatccttcaa ccggaccatc gccctgccct ccgccaggtc ttcgcgtggc
    16441 aggatgactt ctcctgtgaa atggaggtcc cgggtccccc tttccgggtg tgcaggatgg
    16501 aggagggcat gagcgacgtg atcgggtccc ccctgcaccc ttaccggaac aggatcgggg
    16561 acaaagaagt aacggtttga acttgggtcc acgatagcct tgttcctcgc aaagagattc
    16621 tcccatgaaa atgagatatc tgtctcaccg ataccgatgt cgaggaccgc ttcccgcact
    16681 gcctccgggg aaatcccgcg gcgcgcaatg gcacggagcg tcccaaggcg gatgtcgtcc
    16741 catccacggt actcccccgc ggcgatgccg gcccgcatct gcgaggtgga gagcaccacg
    16801 ccctcgatgc ccatgcggcc gtaatggcgg tatacgggag gttcccatcc gaaatagtca
    16861 aagatgtagc gctgcctcct ggtattcgca atatggtcct tcccgcggat cacatgtgtg
    16921 atgccgagca ggtggtcatc gattgccacc gagaagttca tcagcggata catgcacaca
    16981 tccatccggg ggtgcggggg gcttttgagg atccggaagg ccgggtagtc cctcatcgca
    17041 gggtcgggat gatcgagatc ggtcctgacg cggagagtaa cttcgccttc gccgtactct
    17101 cccgcgcgca tcttctcgaa caggacaaga tcctccccaa tacctcgtcc gcggcatggg
    17161 caggccttcc ttgccagctt catctctttg aaacgctcgt tgtcgcaggt gcagacatac
    17221 gcgccgcccc tctcgatcaa ctcccgcccg taacgataat agatatcaaa ccggtcgctc
    17281 tggtacacga tgtcgctgat tgctagacca agccactcga tgtcctcctt caccattgcg
    17341 tatgcatgag ggtccaccct ttttggatcg gtgtcctcga tcctgaggac gtagcgcccg
    17401 ccatagcgac ggacataggc atcgttcaag accgccgccc ttgcgtgccc gaggtgaagg
    17461 gggccactcg gattgggggc aaacctcatc acgacaccat tctcggcctt ctcaagggtg
    17521 ggcagcgcct tcgcatgctc gcgggggcgc gacatctcct ccacgagttc cggcgcaaga
    17581 gaacaaaggc gatcagcccg ttccgcaggg gtgagcgcgg tgacctcgtc gatgacctcc
    17641 tttataagtg tcgtcgcctc cctggcccgg ggtttcagtt cagggtactt ccccatcacc
    17701 atcccgatca cagtcccagc tttcgggatg ctctggtgct tcactgcgtt ctgcagtgcg
    17761 aagaggagga gcgctctcct caacgggtcc tccatctcaa tagctccggc tgatgaagtg
    17821 gtcacccata tggatgagaa ggtcgcgctc ctcgctctct gggaggatgg atatgcgctt
    17881 ttggccggtc ttcaccaggt tccgggcggt atcctgcacc tccgagatca cacctatccc
    17941 gcggagacgc gtgataatct cctggatctc ggcctgcgag agccttcttt ggaattgact
    18001 gagatcgaac cccttctcca gcgccttgat atggatcagg gtctttttcc cctcccggat
    18061 gtcagatgcc tggtccttcc cgcttacctc cggggacgcg agaaggtcga tgaggtcgtc
    18121 ctggatctgg aatgccatcc cgataccctg cccaaagtgg tagagggcat cggtgaaatt
    18181 cgggtttccc ccggcaagga tacttccgat gccggctgca gccgcgtaca gcgcaccagt
    18241 cttcttctcc accatcttca tgtattcacc ctcgctcaca tcgtcccgct tctcgaaggc
    18301 catgtccatg tgctggccct cgcagatctc cacgcaggtc cgggcaagca tcgaggtggc
    18361 cctgaccttt gcattgtcgg cagcgtctgc aaggctgata aattcaaatg cgctcgcaaa
    18421 gagcacgtct cccgcaagaa tagcggtggg ctcgtcccac tgtgtgtgca cggtgggaac
    18481 tcccctcctc accaggtcgc cgtccatgat atcgtcatgg atgagggtga aactgtgggt
    18541 gagctcgagc gcgagtgccg cggggaccac gtccacagag ctccccctgt tcaccgcatc
    18601 tgcggagagc aacagcaatg ccgggcggag cctcttgcct cccgcaagaa gcaggtgcgc
    18661 gcttgccctg cccaggtcat cgcatgacga tccgaaatac cggtgaataa gggagtcgac
    18721 ctgattggcg gtctgctcca ggtaactatt cagctccatc tcctcttcgt cctccttgcc
    18781 ttatgtcatc ttcacccgct ggccgtttga caggatatgc acatcgttgt gaagcgtata
    18841 cccgatcgcc tcggtgaact ccgcgtatcc tgatgtcatc cggatgtgcc cgtgggccgg
    18901 gatgatatgc tgggggttca ggaggtggat aaattcgtag tgatcctccc ggtatgcatg
    18961 tcctgtcacg tgaaggtccg ggaagatgcg tgctcctgcc atgttcaggt gtgcctcgac
    19021 catgtatcgc tgcccgacat tcatcgggtt tgggatcacc ttggcgctga agatcacctt
    19081 gtcgcccttc tccaccttgt agggagtgtc tccaagcgcg atccttgtca ggatagaccc
    19141 gggctcaccc tggtggccgg tgatgatggg gacaaacttc tccttccccg ccttcatcat
    19201 cctccgcagt gtgcggtcaa ctgtcctcct gttcccgaac atgctcatgg tatcagggta
    19261 gctgacaagc ttcatctgct cggcggtgga cgagtatttc tccatggacc tgccgaggag
    19321 gaccggtttt cgtccgatct cgtgggcgca ctctgcgatt gtcttcaccc ttgcaatgtg
    19381 cgaggagaac gtggagacta tgattgcaga cttgtcgtct tcgtaactcg tgattgtgtc
    19441 cctgacaaga tcccttgcga tgcgctcgct cggggcacga cccctgttgt cgatataggt
    19501 cgcctccacg ataagggcga gcactccctc cttcccgatc tgccgcaggc gggcaaagtc
    19561 ggggggctca ccaataaccg gtgtcctgtc gagcttgtaa tcacaggcat acacaatggc
    19621 acctttcggc gtgtgaagca ccgcggtcac cgtatcgatg atgctgtgct gcatgcgtac
    19681 gaattcaagc gtcaattgct gggacagggt gtatctctgg ccggctctca gggcaaacaa
    19741 cttgttgttc accccgaact tctgctctcc ggcaatctgc tgccgtatca gttcagtggt
    19801 gtagggggtg ctgatgatcg gtgcattgta gcggtgggca agcttcggga tggccccgat
    19861 atggtcaagg tgcccgtgag aacagacaat cgccttgaca cttccctcca ccgtgttcat
    19921 catggtgtcg tccgggattg ccttcatctg gataaggtcc agggagtgca tgttctcgac
    19981 ttccgcctcc tcgtggatca tcacctggtc cagacggagc cccatgtcaa aaataacaat
    20041 gtcctttcca cagcggacgg cggtcatatt tctcccgacc tcgtcgtacc cgcccaccgc
    20101 gattatttca atatccatta ttttcctcc
//
LOCUS       DAOQYR010000034        20051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_3048_length_20051_cov_9.297960, whole genome shotgun sequence.
ACCESSION   DAOQYR010000034 DAOQYR010000000
VERSION     DAOQYR010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 20051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20051
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3048_length_20051_cov_9.297960"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PKM87_05710"
     CDS             <1..737
                     /locus_tag="PKM87_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ29598.1"
                     /translation="QEVTLDRDMTCWEILEFHGSLYRMPHGERRRRIEELLAVVGLDG
                     KRDTLTKHLSGGMKRRLEIARGLMTRPRVLFLDEPTIGLDPQTRRRMWDYIEAVNEEG
                     TTIFLTTHYMDEADQLSDRISIIDHGEIIATGTGWELKNRLGEDVIYMETSENGRAKE
                     ILGAMEEVTEVKEKTNGFVALVNQDGTRLLPKIMDELRGHGVRILTVNLKKPSMDDVF
                     VHFTGRELREERAERPSAAHSRATRR"
     gene            740..1576
                     /locus_tag="PKM87_05715"
     CDS             740..1576
                     /locus_tag="PKM87_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ29599.1"
                     /translation="MHLGFLTIYWRDMIRFVRFRTLLISSLVQPALWMAFFGIAMSAN
                     FNRLTSEVPPVPGIPDVGYLTFMAAGVIAMTTLFTSLFGGMVLLFDKNWGLLREVMAS
                     PLPRDHVIFGIALSGMTKSFIQAIIIMVFGILLGAEFFRGYGAAEVTISLLGILLFVG
                     IFSLGFLFLSAAIAISLESPEGMQGIMTLLTMPIFFASNALYPVNAFPPVLQAVSVGN
                     PLTHLVNGIRYFAIGDHYQAIGIVYTTTPADMLISFFVLVVFALVMFGLAWWRFQKAV
                     VT"
     gene            complement(1578..3722)
                     /locus_tag="PKM87_05720"
     CDS             complement(1578..3722)
                     /locus_tag="PKM87_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNQ29600.1"
                     /translation="MNSIFSGLHGSLQEVLAHRLGWSDLREVQSRTYREVAEGKDVMV
                     IAPTAGGKTEAALIPVLDCVLKEGSPGVACLYISPLKALINDQEDRFRSFAVPTGLEV
                     RIWHGDIPRGDRIWEDGEPPHLLMITPESLEVLMGEMIQSRDLRHARFVIVDELHAFV
                     ESERGAHLRVLLDRLDRIAGRHVQRIGLSATVGNPDEVLAWFSGGMHKKELVQVAIPP
                     REKRFSFHVEGDERRRMAAIARLVAGRKALVFANSRAEAEQVGRALHGHVSQLFVHHS
                     SLSSEMRRAAEESMAGEASACIICTSTLELGIDIGDLDVVVQLGAPASVSSFLQRMGR
                     SGRRGRSPYMAFVLADAWDLLLCTAVIESASRKKVEPLIPAKRPYNVLVQQVFLETVR
                     HRRTTRARLKKFLGSLPSLKDLPPHTVDLLIAHLEGGKFLVRDGEMFMPGPQMEMLYG
                     RSNWKDLYSVIRGGGEFRAVTPDGDLVGRLDARFVASRGEGSFSLGGKNWTLVKSDES
                     HNLVVVVPGEEAGGKAFWTGGQAGFSPVVCQAVQRILARGRTLLPLLPAEEEVLDGAM
                     AGIPRIHARGIHIWEVPGKRKMDAIALTFLSRADNALLSTMAKKLHGEKTRVRYDDLS
                     LWFPDLAQEGAAERVLSILRQVQVLPADEMAGLLSMPAPETWKFGPALPAAQLREMAA
                     VDTWHLAEFVARFCSLPIYQVSAPGMECGQER"
     gene            complement(3732..3977)
                     /locus_tag="PKM87_05725"
     CDS             complement(3732..3977)
                     /locus_tag="PKM87_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29601.1"
                     /translation="MRETSGKLQGYAATRGPAERQVPYLNRKEKKNTPTWRRTGVLGA
                     TGVLSMGVYLINGENKWYLCVSFPSSTTDNRVQHKTP"
     gene            4090..5655
                     /locus_tag="PKM87_05730"
     CDS             4090..5655
                     /locus_tag="PKM87_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNQ29602.1"
                     /translation="MAASLFWLAIVTAIYLVAILCLGYLGYKKTKAAEDYLVAGRNAH
                     PVVIALSYGATFISTSAIVGFGGQAANLGMGMIWLTVFNIGVGVLLAFVIFGKKTREI
                     GSRVKAITFPDLMGKIYSSQFMQVVSGLVIIVGMPLYTAAVLIGGAQFIKETLGISYS
                     ASLIGFAIIVAIYVIFGGLIAVMYTDAMQGAIMLIGMTFLLIFTYAILGGVTAAHNAL
                     TAMADMIPVKLAEQGMNGWTVMPDLGSPIWFTLITTLVLGVGIGVLAQPQLVVRFMTA
                     KDGRSLNRAVLVGGPFILMMTGVAFTVGALTNVYFNQTQGTIAVAAAGGNVDAIIPLF
                     INLATPDWFTVLFMITLLAAAMSTLSSLFHAMGTALICDVWGRGRECALSLKANQAGV
                     LVMIIASVIVAFLMPGSIIAIATAMFMGLCASAFLPVFAVAVYCKKPDPFAAKVSLVV
                     GAAVWFFWALFVNSRYAAVFGLSSALRGTTSLLPYPWSAIDPLIIGLPLSALAILVLQ
                     ARKNRNRGESAPA"
     gene            complement(5910..6560)
                     /locus_tag="PKM87_05735"
     CDS             complement(5910..6560)
                     /locus_tag="PKM87_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HNQ29603.1"
                     /translation="MPPTIRAMTRVLLPIILGILYIAACALFLPQESALLLAGLMLAY
                     VIPPAGKESVIPLGIALGFPWWLIGTSIALLDVLAGLFMALNFELAFNVPLLGGWISR
                     FMDHGQAFLSRRPYLKKFYFSGLVLFVMFPLQGSGGIGGSLAGRLLGMPRGTVLLAIT
                     LGAFLGCYLIALGSEFIWELVITRPALGITVAVAVIATILAGYLAYRRRMPRVSDE"
     gene            complement(6754..7278)
                     /locus_tag="PKM87_05740"
     CDS             complement(6754..7278)
                     /locus_tag="PKM87_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29604.1"
                     /translation="MVAARYEKSENIVQGSLREYDRLMKFFQRPLFLSLTIGVPFCIF
                     KLLFGMVAIQVVTFPYHGVLAVFGWVVVLWAGTDLVMNAAKALFDLFDRQAPFEYCTI
                     AQMGACFHMPLVFLALDTLLSFVIICVMLWSGWITLLTPVESYFWYAATTMNLISLSL
                     VMLYNEVRKVRSVS"
     gene            complement(7366..8616)
                     /locus_tag="PKM87_05745"
     CDS             complement(7366..8616)
                     /locus_tag="PKM87_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ29605.1"
                     /translation="MKQLGVAVIGGGPAGLFCAICSAAAGKKVAVLEKMPTCGRKLLI
                     SGTGQCNITHDGDVSSFFTHYGAHGKFVKPALLQFSNRDLVDFFRKSGLDMVTEPGGK
                     VFPVTRRAGDILSVLTGECTRRGVDIRCNEPVLGTEQCEGGFTVRTGEGVYFTRNLVV
                     ATGGATYPATGSTGDGYALARSLGHPVTEIGPALAAVRVKDFPYPELAGMSFENLPIS
                     VSRQKRLVCRQRGDILFTHTGLSGPGILDISRDIRPADLVEVSFISTLAPAEARNAVH
                     DALLGGKGMHVATVLRGLNLPERLAKRIPEHAGVPAGATCAHLSKGARNAIAVLVSGH
                     PFEVAGLAGFDQAMVTRGGVALDLVNPRTMESRLHPGLYFVGEVLDIDGDTGGYNLQF
                     ACASGVLAARSITRTAMNQRTTMP"
     gene            8809..9420
                     /locus_tag="PKM87_05750"
     CDS             8809..9420
                     /locus_tag="PKM87_05750"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNQ29606.1"
                     /translation="MKSANFPLFSPFKPEKSLVNMFHVLFIVIMLVFGGPLLVGAYFS
                     ESATFLAACLVMLFAVAGWYLFWVGKYYQTIFFELKDDEVTWKRGVWFRKTGIVPYDR
                     ITNIDIYQGPLMRHFGFSSIKLQTAGYSAQARSEITLEGIAAAEELRETIRSLIRESR
                     RVSGSSDATGSLSRPHAPPQSEPVLLGELRAIRLLLERIEKKS"
     gene            complement(9448..10032)
                     /locus_tag="PKM87_05755"
     CDS             complement(9448..10032)
                     /locus_tag="PKM87_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HNQ29607.1"
                     /translation="MEKTRIIVGLSGASGIVYGIRLLQAARELGLETDLIMTDLAAKM
                     IEIETETDPSEVIALATRVHDSFDFTSPLASGGFSHRGMVVVPCSMKTVAGIACGFAD
                     NLLLRAADCALKERRPLVLVPRETPLSGIHLRNMLTLSEAGAVILPASPGFYHRPRDI
                     GGLVDHVVGKVLDVLSVEHHLYRKWREGEGGSRA"
     gene            complement(10347..10766)
                     /gene="trxA"
                     /locus_tag="PKM87_05760"
     CDS             complement(10347..10766)
                     /gene="trxA"
                     /locus_tag="PKM87_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNQ29608.1"
                     /translation="MEDEELQRIREKKRKELEERLHAPHEMQPSAKVLLVEEANFRSL
                     LSQHPRFVVDFWAEWCGPCRMVGPVIDALCQEMAGKVIFGKCNTDHNPLIAAQFGISA
                     IPTVLLFNGGQLADRIIGAYPKEAIKTRICRAYGLPP"
     gene            10889..10963
                     /locus_tag="PKM87_05765"
     tRNA            10889..10963
                     /locus_tag="PKM87_05765"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10924..10926,aa:Met,seq:cat)
     gene            complement(11045..12466)
                     /gene="purF"
                     /locus_tag="PKM87_05770"
     CDS             complement(11045..12466)
                     /gene="purF"
                     /locus_tag="PKM87_05770"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNQ29609.1"
                     /translation="MCGIVGIRDAGGVSFSIYYSLYALQHRGQESAGISTFDGTRLYK
                     HKGQGLVAEVFDSTILESLEGNVGIGHVRYPTTGANRPENAQPFNFVYRDHIISIAHN
                     GNLVNSRELREEYECRGQIFCSTTDTELIAKVITEEMSNSGCIEDAVHRCMRVLKGSY
                     SVVMMIDGTLYGFRDPLGIKPMCIGKAGQALVLASESVAIDALAGTFLRDVTPGELIC
                     IDDQGMRSMQIAVSRRKAHCIFEYIYFARADAVLDGALVYDVRLKIGQKLFEEEPVKA
                     DSVCPVPDSGTAYAIGYSARSGTPFVESLLKNRYMGRTFIMATQKEREMAVRIKLNPI
                     REHLDDKSVVLVDDSIVRGTTSRRIIGIMRDAGAREIHVRIGSPAIKAPCYLGVDMPT
                     REELIASDKEEEEVRKRITATSLHHISIDALVEAIGIDRANLCTGCLTGCYPVDITGE
                     KSSPPPVDFLDGTYQTRLGSFGS"
     gene            complement(12476..12658)
                     /locus_tag="PKM87_05775"
     CDS             complement(12476..12658)
                     /locus_tag="PKM87_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37e"
                     /protein_id="HNQ29610.1"
                     /translation="MSKGTPSMGKRNHKTHIACRRCGRVSYHLRHKTCSACGFGKSTK
                     IRGYKWTMKRPKVPTH"
     gene            complement(12702..12932)
                     /locus_tag="PKM87_05780"
     CDS             complement(12702..12932)
                     /locus_tag="PKM87_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNQ29611.1"
                     /translation="MTKRPLDILDQVLNRQPVIVSLKGGRELRGVLQGYDVHMNLVLD
                     KAEEIENGQARSVGTLIVRGDNVIYISPSLES"
     gene            13130..13627
                     /locus_tag="PKM87_05785"
     CDS             13130..13627
                     /locus_tag="PKM87_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNQ29612.1"
                     /translation="MSRITPRKRHTIRRSQAQELLSSLREQIGDAAEMFSAERMEILE
                     TDAAISLYLVDRKPLLMRLQGMVFPTLRGLLEHPFPSRRVVVDSGAVPFVIKGADVMR
                     PGITSISDDVTAGMPVQVVEERHGKPIALGIALFDACEMREKNTGKVIKTLYYVGDDL
                     WSLEI"
     gene            13751..14122
                     /gene="sepF"
                     /locus_tag="PKM87_05790"
     CDS             13751..14122
                     /gene="sepF"
                     /locus_tag="PKM87_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HNQ29613.1"
                     /translation="MVKFLDTLLGKSTPSSEDDYMDLDLEAFEEAEAGAPALFVKIAS
                     VGDIKDSPRVKDEIYNGNIVIVDIAKLKADKVMYERVLKDLKEVAKDVNGDIIGLGDQ
                     RYVVITPNSVKISRDKIGGVS"
     gene            14126..14677
                     /locus_tag="PKM87_05795"
     CDS             14126..14677
                     /locus_tag="PKM87_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZPR1 zinc finger domain-containing protein"
                     /protein_id="HNQ29614.1"
                     /translation="MRQVVPGPCPACGTEIEYIYQTEEIPYFFEILIISALCPTCGYR
                     LADTELLKNAEPSRWELTIESPADLDIRVVRSMNGVISIPELGVHVDPGPACEGFVSN
                     VEGVLNRIDAVLESVIGWTDDDDERRNALQLREQIAEVKKGRLPVTLRIDDPTGNSAI
                     LGDRARIYPITAAECGYGPDTAE"
     gene            complement(14683..15678)
                     /gene="purM"
                     /locus_tag="PKM87_05800"
     CDS             complement(14683..15678)
                     /gene="purM"
                     /locus_tag="PKM87_05800"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNQ29615.1"
                     /translation="MSSPRTYRDAGVDIGLEARAISALVQNLSFRRRGAYPMMGGVGH
                     FAGLIDFGDIALALTVDGVGTKMLVADRLQDWSTVGIDCMAMNVNDLYVMNMEPVAFV
                     DYIATDRLSVGQMAQIGEGLNEGARQANVNIVGGETATLKGLVNGLDLAGTCLGVQRK
                     ERVIAGEKIRPGDLVVGLPSSGIHSNGLSLARRVVESHDAWDETLSSGRSIGRELLVP
                     TRIYHEVLDLARETEIHGMCHVTGGGLLNFSRLTGFGFEFTDPMPPQEIFSWVQEHGE
                     VERAEMYRTFNMGMGFACIVPEGAEGPVLKRVRGSQVAGVVTARPGIYLEEEEIR"
     gene            complement(15671..17059)
                     /locus_tag="PKM87_05805"
     CDS             complement(15671..17059)
                     /locus_tag="PKM87_05805"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNQ29616.1"
                     /translation="MKFGGTSVADGTSVSRVVDIVQSCYAAGNEVAVVVSAQRGVTDQ
                     LITMATELANSTDSSGIESFINSLRIRHGKVLLDSAEDYADEVGILLEEQLCNLENIL
                     NAVHNLRELTPRSRDYIITFGERLNSLVVSAAFRQRGIPSMTLDGCEAGIVTTANHGE
                     AMALPMSEGKIQSRVLPLLADSVPVIMGFMGCTDKGVVTTLGRSGSDYSAAIIGAAIN
                     ADEILIWTDVDGIMTSDPRLIPDARVISSISYLEVMELSYFGAKVMHPRSIEPAMNKN
                     ILVRVKNTFNPDHPGTAIVRGEKRDSRVVKALTYIEKVAMVNICGAQMIGRPGVARAI
                     FSQLADRDVNVMMISQGSSEANITLVVEESQEETAREALLALVKQGMVRQVTSNRDVC
                     AVAVVGSGMAGAKGTGGRIFTALGNGGINVMMISQGSSEVNISFVVRQEDGPRAMRIL
                     HEEFHLSEEDNE"
     gene            17133..18758
                     /locus_tag="PKM87_05810"
     CDS             17133..18758
                     /locus_tag="PKM87_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit A"
                     /protein_id="HNQ29617.1"
                     /translation="MRSAELLDCHVLVIGSGGAGVRAAIEASGYGETVLVSKTLTGKG
                     GCTTMAEGGYNAVLREQDSCEIHFEDTMKGGAYLNDPDLVRILVDEAPKRIDDLLQWG
                     AVFDVTDCCEVAQRPFGGQRFPRTCYAGDRTGHEMMATLVERLAGTDVDVMNEVCALD
                     LLKDGDRVLGAVGLTEKDELVAIRADSTVLATGGGTRIYDISTNSSSGTGDGFAIGYR
                     AGAELIDMEMVQFHPTGAVYPYDARGRLITEAVRGEGGVLKNSLGERFMNRYDPERME
                     LSTRDVVSRAIATEILEGRCTGHGGVYLDVTHLPRQQIEEKLPVMLEQFLKFGVDIRE
                     EPMEVAPTAHHMMGGHRITENGETTISGLYACGEVAGGVHGANRLGGNALADTQVFGK
                     RAGEAAGKSGKKSGRIDQRAMDAQEARLSAFFDGEVVPSTVARSLRTAMWEGAGIFRT
                     AKELEKTLGVINHLSSLRLKASGRSNLAECCILQNMLTTASLVVKGALIRKESRGAHV
                     RKDVTQTWDPQTSPYGHSYQSLTQSGIETGVKA"
     gene            18755..>20051
                     /locus_tag="PKM87_05815"
     CDS             18755..>20051
                     /locus_tag="PKM87_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNQ29618.1"
                     /translation="MKKMKVRIQRFDPHQDEKPHYQTYLVEVNEGARVLHVLHAIHDE
                     IDPTLSYRWCCGSGQCGSCAVRINGEPVLACMEEARDGMTIEPLKLPVKKDLIVDLEP
                     ALEALSHLEPGDKPAMPSKEEVELIKPLRSCIECLACVSVCPAMDVTSFAGPTAMRQN
                     LRLALDPRDSKDRVTDAVSKGLFTCTSCQACWKVCPKDIQLPGKAIEKLRSLANRKGL
                     TLPRHQEVEKLVRETGRSVTRTEPTFMEQVGEVIEPYGPVKATVGFFVGCMYNQRLPQ
                     TALDAMEVLRRNGIRVIIPKEQVCCGSPLIRTGQWDYLDTLKRRNIDAFVFRNIDTVL
                     TMCAGCGSTLKNDYRTPFRVLDINELLTKYGIEPPARLPLKATYHDPCHLMRGQGIRD
                     LPRELITQVVDLVEMPAICCGSGGGVRSGVPEEAAALAEK"
BASE COUNT         4116 a         6028 c         5819 g         4088 t
ORIGIN      
        1 tccaggaagt gacgctcgac cgggacatga cctgctggga gatcctggag ttccatggat
       61 ccctctaccg gatgccgcac ggggagcgga ggcggcggat cgaggagctt ctggccgtag
      121 tcgggctcga cgggaagcgc gacactctca ccaagcacct ctccggcggg atgaagcgga
      181 ggctcgagat cgcacgtggg ctgatgaccc ggccgagggt gctctttctg gacgagccca
      241 ccattggcct tgacccccag acgaggagaa ggatgtggga ctacatcgag gcagtcaacg
      301 aagagggcac caccatcttt ctcaccacgc actacatgga cgaggcagac cagttgagcg
      361 atcgcatcag tatcatcgac catggggaga tcattgccac cggaacgggc tgggaactga
      421 agaacaggct tggggaggac gtcatctaca tggagacgag cgagaacggg agagcaaaag
      481 agatcctcgg ggcaatggag gaggtgacgg aggtgaagga gaagacgaac gggttcgtcg
      541 ccctcgtcaa ccaagacggc acccgcctcc tcccgaagat catggacgaa ctcaggggtc
      601 acggggtccg gatcctgacc gtcaacctga agaaaccctc gatggatgac gtgttcgtcc
      661 acttcacggg ccgtgaactg cgcgaggagc gtgcggagcg cccttcggct gcccattcaa
      721 gggcgacgag gcggtgacca tgcaccttgg gtttctcacg atttactggc gtgacatgat
      781 ccggttcgtg cggttccgga cactccttat ctcttccctc gtccagcccg ccctctggat
      841 ggccttcttt gggatcgcga tgtccgcgaa ctttaaccgg ctcacctccg aagtgccccc
      901 ggttccgggg attccggacg tcggctatct cacgttcatg gccgcgggtg tcatcgcaat
      961 gacgaccctc ttcacaagcc tctttggagg gatggtcctg ctctttgaca aaaactgggg
     1021 cctgctccgg gaggtcatgg caagcccgct cccaagagac catgtcatct tcggcatcgc
     1081 cctctcgggg atgacgaagt cattcatcca ggccatcatc atcatggtct tcgggattct
     1141 tctcggcgca gagttcttca ggggctacgg cgccgccgaa gtgacgattt cgctgctcgg
     1201 aatccttctc ttcgtcggga tcttctcact cgggttcctc ttcctctccg cggcgattgc
     1261 catttccctc gagagtccgg aggggatgca ggggatcatg acgctcctga cgatgcccat
     1321 cttctttgca agcaatgcgc tctacccggt gaacgccttt cccccggtcc tgcaggccgt
     1381 atcagtcgga aatcccctca cacacctggt caacggcata cgctactttg ccatagggga
     1441 ccattaccag gcgatcggca tcgtctacac cacaacacct gccgacatgc tcatctcgtt
     1501 ctttgtcctt gtggtattcg cactcgtaat gttcggcctt gcatggtggc ggttccagaa
     1561 ggcggtggtg acctgaatca gcgttcctgc ccgcactcca tgcccggtgc agatacctgg
     1621 tagattggaa ggctgcagaa cctcgctaca aactccgcaa ggtgccaggt gtccacagcg
     1681 gccatctccc ttaactgggc agcagggagg gccggcccga acttccaggt ctccggcgcg
     1741 ggcattgaaa ggagacctgc catctcatca gccgggagaa cctggacctg ccggagtatg
     1801 gaaagaaccc gttctgctgc tccttcctgc gccaggtcag ggaaccagag cgagaggtcg
     1861 tcgtaccgca cccgcgtctt ctcgccgtgc agctttttcg ccatggtgga gaggagcgcg
     1921 ttgtcggccc ttgagagaaa ggtgagtgcg attgcgtcca tcttccgctt ccccggaacc
     1981 tcccagatat gtattcccct ggcatggatg cgggggatgc ccgccatggc cccgtccagg
     2041 acttcctcct cggcgggcag gagcggaagg agcgtccttc ccctggcaag gatcctctgt
     2101 accgcctggc agaccacagg ggagaatccg gcctgcccac ccgtccagaa cgcctttcct
     2161 cccgcctcct cccccgggac caccacgacg aggttgtgcg actcgtcgct ctttacaagg
     2221 gtccagttct tccctcccag ggagaaactc ccctcccccc tgctggccac gaaccgggca
     2281 tcgagccttc ccacaaggtc gccatcaggt gttaccgcac ggaactctcc gccaccccgg
     2341 atcacagagt agaggtcctt ccagttggac cgcccgtaca gcatctccat ctgtggaccg
     2401 ggcatgaaca tctccccgtc ccgcaccagg aacttccccc cctcgagatg agctatcagg
     2461 aggtcgacag tgtgtggggg gagatccttc aggctcggga gcgaacccag gaattttttg
     2521 agcctggccc gggttgtcct cctgtgccgc accgtctcca ggaatacctg ctggaccagg
     2581 acattgtatg gtctctttgc gggaatgaga ggttccacct tcttcctgct ggcgctctcg
     2641 attactgcag tgcacaggag gaggtcccac gcgtccgcca gcacgaacgc catgtagggg
     2701 gaccgccccc tcctgccgct cctccccatc cgctgaagga acgaggagac cgatgcaggt
     2761 gcaccgagct gcaccaccac gtcaaggtcc ccgatatcta tccccagctc gagagtgctc
     2821 gtgcagatga tgcaggcact tgcctcgccc gccatggact cctctgcggc acgccgcatc
     2881 tccgaagaga gtgacgagtg gtgaacgaag agctgggaaa catggccatg gagggcccgg
     2941 cccacctgct cggcttccgc cctgctgttc gcaaagacaa gtgcctttct gcccgcaaca
     3001 agccgggcga tcgccgccat tctccggcgc tcgtcaccct cgacatggaa cgagaacctc
     3061 ttctcccgtg ggggtattgc cacctgcacg agttcctttt tgtgcatacc tcccgagaac
     3121 caggcgagaa cctcatcggg gttccctacc gtcgccgaga gcccgatccg ctggacgtgg
     3181 cgtccggcta tcctgtccag gcggtcgagg agcacccgaa ggtgggcgcc acgttccgac
     3241 tcgacaaagg catgcagttc atccacgatc acgaaccgtg catgccggag gtcccttgac
     3301 tggatcattt cacccatcag cacctccagc gattcagggg tgatcatcag gaggtgcggg
     3361 ggctcgccgt cctcccatat acgatcaccc ctgggaatgt ccccgtgcca tatccgcacc
     3421 tcgagcccgg tggggaccgc gaacgacctg aaccggtcct cctggtcgtt gataagggcc
     3481 ttcaggggtg agatgtacag gcaggccact cctgggctcc cctccttcag gacacagtca
     3541 aggaccggga taagcgcggc ctcggtcttt cctccggcgg tgggggctat gaccatcacg
     3601 tcctttcctt cggccacctc gcggtatgtc cgtgactgga cttcccggag atcggaccat
     3661 cccagccggt gggcaagcac ctcctgcagc gacccgtgca ggccggagaa tatcgagttc
     3721 accccttctc atcaaggtgt tttgtgctgc acccggttat ctgttgtgct ggaaggaaaa
     3781 gaaacgcata aataccattt attctcacca ttgatcaggt aaacacccat ggatagcacg
     3841 cccgtggcac ccagcacacc ggttctccgc cacgtcggcg tgtttttctt ctccttcctg
     3901 tttaggtagg ggacctgcct ctctgccggt ccccgtgtag cggcatatcc ctggagttta
     3961 ccggaggttt ctctcatgct tggaattcct gacccacaaa tatggatcgc gtatctgctc
     4021 tgcatcgcgc ttgcgcttgc ttgcatactc tatggtctcc tcaactggaa ccaggggggc
     4081 gagtgaacaa tggccgcaag cctcttctgg ctcgctatcg ttactgccat ctacctcgta
     4141 gcaatcctct gtctcggtta cctcggctac aagaagacga aagccgcgga ggactacctc
     4201 gttgcgggcc ggaatgccca tccggtggtc atcgccctct cttacggtgc cacgttcatc
     4261 agcacgtctg ccatcgtagg cttcggaggc caggccgcaa acctcgggat gggcatgatc
     4321 tggctcaccg tctttaatat cggcgtgggc gtgcttctcg cgtttgtcat cttcggcaag
     4381 aagacccgag agattggctc acgcgtgaag gccatcacct tccctgatct gatgggaaag
     4441 atctactcct cgcagttcat gcaggtggtc tcgggcctcg tgatcatcgt cgggatgccg
     4501 ctctacactg ccgcggtgct gatcgggggt gcgcagttca tcaaggagac gctcgggatc
     4561 tcctattctg catcactcat aggctttgcc atcatcgtgg ctatctacgt catattcggc
     4621 gggttgatcg cggtcatgta taccgatgcc atgcagggtg cgatcatgct gatcggaatg
     4681 acctttctcc tcatcttcac ctatgcaatt ctgggagggg ttacggccgc acacaacgcc
     4741 ctgacagcca tggccgacat gataccggta aaactcgcag agcagggaat gaacggatgg
     4801 accgtgatgc ccgatctcgg gtctcccatc tggttcaccc tcatcacaac cctggtgctc
     4861 ggggtaggca tcggggtgct ggcccagccg caactcgtcg tgcggttcat gaccgcaaag
     4921 gacggccgtt ctctcaacag ggcggttctc gtcggagggc ctttcatcct gatgatgacc
     4981 ggggtcgcat tcacggtcgg cgccctcacc aacgtctact tcaaccagac gcaagggacc
     5041 atcgcggtcg ccgctgcggg cgggaacgtg gacgccatca tccctctctt catcaacctt
     5101 gcgacccccg actggtttac ggtcctcttc atgatcaccc tcctcgctgc agcgatgtcc
     5161 acactctcct cgctcttcca tgcgatggga acggcgctga tctgcgacgt gtgggggagg
     5221 ggaagggagt gcgcactctc gctcaaggcc aaccaggccg gcgtactcgt catgatcatc
     5281 gcaagcgtca tcgtcgcctt cctgatgcca ggcagcatca ttgcgatcgc cacggcgatg
     5341 ttcatgggcc tctgcgcctc ggccttcctc ccggtcttcg ccgtcgccgt ctactgcaag
     5401 aaaccggacc cctttgccgc gaaggtgagc ctcgttgtcg gagcggcagt ctggttcttc
     5461 tgggcactct tcgtgaactc ccggtacgca gccgtattcg ggctctccag cgcgctgcgt
     5521 gggaccacga gtctcctccc ctatccctgg agtgcgattg acccgctgat cataggcctg
     5581 cccctctcgg cactggcgat cctcgtgctc caggccagga aaaacaggaa caggggtgag
     5641 agtgccccgg cctgaatact ttttttctct ctcgtttcaa tcgtggccat ttccctctgg
     5701 caggacgttg tcgcccccat gtgagccatt tattctgtga ttcacgaggg aatacgaagg
     5761 ggacgcgggt ataatcgcat tatgggtata tggacactta aatacaggag atacccggca
     5821 aggatgagga gtgcccatat ccggagcatc catgtcacca taaattggaa cagaagaaca
     5881 atgcatgggt ggtaatgaga gtggtcatcc tactcgtcgc tcaccctggg catcctcctg
     5941 cggtatgcca ggtaccctgc gagaatcgtt gcgatgactg cgaccgcaac cgtgataccg
     6001 agggcgggtc gtgtgatgac aagctcccag atgaattccg atccgagcgc gatcaggtag
     6061 cacccaagga atgccccgag ggtgattgcc aggagcacgg ttcccctcgg catcccgagg
     6121 agccttcctg caagggaccc cccgataccg cctgatccct ggaggggaaa catcacgaag
     6181 agcacgaggc ccgagaagta gaacttcttt aagtacggcc ggcgggacag gaacgcctgg
     6241 ccgtggtcca tgaaccggga tatccacccc ccaaggaggg ggacattgaa ggcaagttcg
     6301 aagtttaagg ccatgaagag gcccgcaagc acatcgagga gggcgatgga ggtgccaatg
     6361 agccaccacg ggaatccaag cgcgatgcca agggggatga ccgattcctt tcccgccggt
     6421 gggatcacgt aggcaagcat gagccctgcc aggagaagtg cgctctcttg tgggaggaag
     6481 agggcacagg cagcaatgta aaggatccca aggatgattg ggaggaggac cctggtcatc
     6541 gcacgaatgg tagggggcat ggtggattct gccaaatggg ttccagagct acatctcctc
     6601 agttcaacat caacatgcgc atccccgatg caacgcactc cgcactgagt cccataggaa
     6661 caccggatta acctgaaggc tcatccatac ctatcgatca cgaatgtgtt ggtcaggggc
     6721 ccagggggtc tgtactgata ccaatcccct ggctcatgat acagaccgca ccttccgcac
     6781 ctcgttgtag agcatcacga gcgagaggct tattaggttc atggtggtcg cggcatacca
     6841 gaagtacgac tcgacgggag taagcagggt tatccaccct gaccagagca tgacgcagat
     6901 gatgacaaat gagagcaggg tatcaagggc gaggaagaca agaggcatgt ggaaacaggc
     6961 tcccatctgg gcaatggtgc agtactcgaa tggagcctgt cgatcgaaca ggtcaaaaag
     7021 cgcctttgcg gcattcatga ccaggtcagt ccctgcccag agaaccacca cccacccgaa
     7081 gactgcaagg accccatgat atggaaaagt caccacctgg attgcgacca tgccgaagag
     7141 gagcttgaat atgcagaacg ggacaccgat ggtaagcgag agaaagagcg ggcgctggaa
     7201 gaacttcatc agccggtcgt actcgcgcaa cgacccctgc acgatgttct cactcttctc
     7261 ataccgggca gcaaccattg ttcatctcct ttgcagatcc gggtttttgg tgaatagtgg
     7321 ttctttgggg gagatatagc ccccagggag aaagacccgc cgtcgtcatg gcatggtcgt
     7381 ccgctggttc atcgcagtcc tggtaatgct ccgtgcggcc agcacccccg atgcgcaggc
     7441 aaactggagg ttgtaacctc cggtatcccc atcaatgtcg agtacttccc cgacgaagta
     7501 gaggcccggg tggagccggg actccattgt ccgcggattg acaagatcga gcgcaacgcc
     7561 accccgggtc accattgcct ggtcaaagcc ggcaaggccc gcgacttcaa acgggtgccc
     7621 gctcaccagc accgcgatgg cattcctggc acccttcgag agatgagcgc aggtcgcccc
     7681 ggccggaacc cctgcatgct cgggaatcct ctttgccagc cgttccggga gattaagccc
     7741 tctcaagacc gtcgcaacgt gcatcccctt ccccccaagg agagcatcat gcacggcgtt
     7801 cctggcctcc gcgggcgcaa gggtggagat aaacgagact tcgacgaggt ccgccggccg
     7861 gatatccctc gagatgtcca gtattccagg gcctgagagg ccggtatggg tgaagaggat
     7921 gtctccccgt tgacggcaga caagtctctt ctgccgcgag acactgatgg gaagattctc
     7981 aaacgacatc ccggccagtt cgggatatgg aaaatctttc acccgcaccg cggcgagggc
     8041 cgggccgatc tctgtaacgg ggtggccaag cgacctggcc agtgcatacc cgtcaccggt
     8101 ggaccctgtg gccggatagg tggcccctcc tgtcgcgacg acgaggttgc gggtgaaata
     8161 cactccctct cctgtcctca ccgtaaaccc gccctcgcac tgctctgtgc caaggaccgg
     8221 ttcgttgcag cggatgtcca ctcccctgcg ggtgcactcc ccggtcagga cagagagaat
     8281 gtcgcccgct ctcctcgtta caggaaagac cttgcccccg ggttcggtga ccatatcaag
     8341 ccctgatttc cggaagaaat caacgagatc gcggttggag aactgcagaa gcgcaggctt
     8401 cacaaacttc ccgtgcgccc catagtgggt gaaaaatgag gatacatccc catcatgggt
     8461 aatgttgcac tgccccgtcc cggagatgag gagttttctc ccgcaagtgg gcatcttttc
     8521 aaggactgca accttctttc ctgcagcggc gctgcagatg gcgcagaaga ggcctgcagg
     8581 ccctcccccg atcaccgcca cccccagttg cttcacctct ccctccaggg gcgcccgtgc
     8641 ccggaacggg agtctccagg tgagcgccct ttgttgtgga aggtctcggg atggcagagc
     8701 atcgtaccta acctgtccga ccgcgccggc gtaatagtta tgcccgcagg gatctcacta
     8761 cacagatcag ttctgcagcc atttccgccc gctggaggtg taacccacat gaagtcggcc
     8821 aatttccccc tgttttctcc gttcaagccc gagaagagcc tggtgaacat gtttcatgtc
     8881 ctcttcatcg tgatcatgct ggtcttcgga ggacctctcc tcgttggtgc atacttctcg
     8941 gaatccgcca cgttcctcgc tgcatgcctg gtgatgctct tcgctgtcgc cggctggtat
     9001 ctcttctggg tgggaaaata ctaccagacc atcttttttg agctgaagga cgacgaggtg
     9061 acgtggaaga gaggcgtgtg gttccggaag accgggatcg tcccatacga ccgcatcacc
     9121 aacattgaca tctaccaggg gccgctgatg cggcatttcg ggttctcaag catcaagctt
     9181 cagaccgccg gctactccgc acaggcaagg tccgagatca cccttgaagg catcgcggca
     9241 gcggaggagc tgagggagac catcaggtcg cttatcaggg aatcaaggag agtatcgggt
     9301 tcttcggatg cgacaggttc actatcccgc ccacatgccc cgcctcagag tgagcctgtt
     9361 ctcctcggcg aactgagggc aatcaggctg ctgctcgaga ggattgaaaa gaagagctaa
     9421 aaaggcgtgt gtgtgtcccc cgttccctca cgcccggctc ccgccttccc cttctctcca
     9481 cttccggtac agatggtgct cgacagagag gacatcaagc accttcccta ccacatggtc
     9541 cacgaggccc ccgatatcgc ggggtctgtg gtagaacccg ggagacgcag ggaggataac
     9601 agctcccgcc tcggagaggg tgagcatgtt gcggaggtga atccccgaga gtggcgtctc
     9661 ccgggggaca aggaccaggg gtcggcgctc cttgagagca caatctgctg cacggaggag
     9721 gaggttgtcg gcaaacccgc acgcaatccc tgccaccgtc ttcatgctgc aggggacaac
     9781 gaccatcccg cggtgcgaga acccacctga cgcgagaggc gaggtgaagt cgaaactgtc
     9841 atgcaccctg gtagccaggg caatgacctc tgacgggtct gtttccgtct cgatctcgat
     9901 catcttcgcg gcaaggtcgg tcatgatgag atcggtctcc agccccagct cgcgtgcggc
     9961 ctggagcagg cgaatgccgt atacgatgcc gcttgcaccg gagagcccaa cgataatccg
    10021 ggtcttttcc atcgggtaca gtggtgatcc ggcggaactt aatatggttg tgatggtgga
    10081 ttcttcaaaa aagcctcctc gctgttttgt gtggggagac tgaatcgagg ttgttatctg
    10141 catatcaatt cagtcactcc aaccgccgcg ggggcgcccc gtcgtgggcg gcggcgtgca
    10201 tcgcatatgg gggtgtgggg gcggacagcc cccacaatat gtgtccggtt caccaatcct
    10261 acccccacca gatagcaaag agcctcaaaa aagatcattt ggggcagggc aggcgccctc
    10321 cctcatttac cggacggtgc gccccctcac ggggggaggc cataggccct gcagatccgg
    10381 gtttttatcg cttctttagg atacgcccca ataatccgat ccgcaagctg ccctccgttg
    10441 aagagaagga ctgtcggaat ggcagagatg ccgaactggg ccgcgatcag ggggttatgg
    10501 tctgtgttgc acttcccgaa tatgaccttt cccgccatct cctggcagag cgcgtctatc
    10561 accggcccga ccatcctgca tggaccgcac cactccgccc agaagtccac cacgaaccgc
    10621 gggtgctggg agaggagcga tcgaaaattc gcctcctcca cgaggaggac ctttgcggag
    10681 ggctgcatct cgtgcggagc gtgaagcctc tcttcgagct ccttccgctt cttctcacgt
    10741 atacgctgca attcctcgtc ttccatggtt attttcgtgt tgggagcgag aacagataat
    10801 cactcccccg ccatgacctg ccggaacaag tctcgagggg caaactatat caccgcagat
    10861 tgtcaactat gttgcgcgaa tccgcggaag cgaggtgggg tagtcaggat atcccgacgg
    10921 gctcataacc cgtagatcga tggttcaaat ccatccctcg ctatttttga tttttacgtt
    10981 catctttcgt gcgaccgcaa ggtgcctcgt ttttaaaaaa gggaaaggtc caggagaggc
    11041 actttcagga cccgaacgac ccgagccgtg tctggtaggt cccgtcaagg aaatcgaccg
    11101 gtggtgggct gctcttttct ccggtaatat ccacagggta acagccagtc aggcacccgg
    11161 tgcaaaggtt cgcccggtcg atgccgatcg cctccaccag ggcatcgatt gagatatgat
    11221 ggagagacgt cgcggtgatc ctctttcgaa cctcctcctc ctccttatcg cttgcaatca
    11281 gttcctcccg ggtgggcatg tcaactccaa ggtaacaggg ggccttgatg gcaggggagc
    11341 ctattctcac atggatctcg cgggcaccgg catctctcat gatcccgatg atccggcggg
    11401 atgtggtccc ccgcacgatg ctgtcgtcca ccagcaccac ggacttgtcg tccaggtgct
    11461 cccggatggg gttcagcttg atacgtactg ccatctcgcg ctccttctgg gtagccatga
    11521 tgaaggtccg gcccatgtac ctgttcttca ggaggctctc gacaaagggc gtgcccgaac
    11581 gggccgagta cccgatggca tatgcagtcc ccgagtccgg caccgggcag acagaatccg
    11641 ccttcaccgg ttcctcctcg aagagtttct ggccgatctt caggcggacg tcgtatacaa
    11701 gtgcgccatc gaggaccgcg tctgcacggg caaaatagat gtattcgaag atacagtggg
    11761 cctttctccg tgagaccgcg atctgcatgc tgcgcattcc ctggtcatcg atgcagataa
    11821 gctccccggg ggtgacgtca cggaggaatg tcccggcaag ggcgtcgatg gccacgctct
    11881 cagatgcgag gaccagcgcc tgacccgctt ttcctatgca catgggcttt atcccgagcg
    11941 ggtcccgaaa tccatagagg gttccgtcga tcatcatcac gaccgaataa gagcccttca
    12001 atacccgcat gcaacggtgc accgcgtcct cgatacaccc cgagttgctc atctcctcgg
    12061 tgatgacctt tgcaatcagt tctgtgtcgg tggtcgagca aaagatctgg ccccggcact
    12121 cgtactcttc cctcagttcc cggctgttga ccaggtttcc gttatgggcg atcgaaatga
    12181 tatggtcgcg gtagacaaaa ttaaacggtt gtgcattctc cgggcgattc gcaccggtgg
    12241 tcgggtatcg gacgtggccg atacccacgt ttccttcaag gctctcgagt atggtagaat
    12301 cgaatacctc ggccacaagg ccctggccct tgtgcttgta gagccttgtg ccgtcaaacg
    12361 ttgagatacc tgcgctctcc tgtcccctgt gctggagagc ataaagcgaa tagtaaatag
    12421 agaaggaaac accgccagca tccctgatgc caacgattcc gcacatagga ggcctttaat
    12481 gggtcgggac cttcggcctc ttcatcgtcc acttataccc gcgaattttc gtgctcttcc
    12541 cgaatccaca ggcagagcag gtcttgtgcc gcaggtggta ggagacgcgc ccgcacctgc
    12601 ggcaggctat atgcgtcttg tgattcctct ttcccattga cggagttcct tttgacatgg
    12661 ttcacctttt ttttagagat ggatattccc ttatagactg tttatgactc gagggacggc
    12721 gagatgtaaa tcacgttgtc cccgcgcacg atgagggtcc cgacgcttcg ggcctgcccg
    12781 ttctctatct cctctgcctt gtcgaggaca aggttcatgt gcacatcata accctgcagg
    12841 accccgcgga gttccctccc gcccttgagg gatacgatga caggctgccg gttcagcacc
    12901 tgatccaaaa tatccaacgg ccgtttggtc ataatcgtac ccacttctag tcactttggg
    12961 gtaatatatg tgcggtccac ggatacttaa atgtactgtc ttatacggag gcgtttccca
    13021 gccttcattc acctattgag ggcattaatg ggcaataaag aagaaaattt ctccccttct
    13081 gggatggatg cgtttttttt gtgtgtgcag caatatgaat atgaactgaa tgtcgcgaat
    13141 caccccgaga aaacggcata caatccggag atcgcaggcg caggaactcc tctccagcct
    13201 ccgggagcag ataggagatg ccgcagagat gttctccgcc gagcggatgg agatcctcga
    13261 gacggatgcc gctatctcgc tgtacctcgt agacaggaaa cccttgctga tgcgtctcca
    13321 gggaatggtc tttcccacgc tccgcggtct cctcgagcac ccgttccctt cacgcagggt
    13381 agtggtggat tccggtgcgg tgccgttcgt gatcaagggt gcggacgtga tgcgcccggg
    13441 tatcacttcg atctcggacg acgtgactgc aggcatgccc gtccaggtag tcgaggaacg
    13501 gcacggaaag ccgattgccc tcggcatcgc gctctttgat gcatgcgaga tgcgtgagaa
    13561 gaacaccggg aaagtgatca agacgctcta ttacgtggga gacgacctct ggtctcttga
    13621 gatctgatgc ggatgtccgg cactcttttg ccgcaccacg gacactcaat cgggaaacta
    13681 aggcttccca agaggaaata tgccaattta catagatact attaaataaa tgacaataga
    13741 caattttcct atggttaaat tcctggacac ccttcttgga aagagtaccc cctcgtcgga
    13801 agacgattac atggaccttg acctcgaggc attcgaggag gcggaggcag gtgcacctgc
    13861 actgttcgtg aagattgcca gtgtcgggga tatcaaggac agtccgcgcg tcaaggatga
    13921 gatttacaac ggcaacatcg tgatcgtcga tatcgccaag ctgaaggccg acaaggtcat
    13981 gtacgagcgg gtgctcaagg atctcaaaga agtggcaaaa gatgtcaatg gggacatcat
    14041 tgggcttgga gaccagcggt acgtggtcat caccccgaac tccgtgaaga tctccaggga
    14101 caaaatcggc ggggtcagct gaagagtgcg gcaggtggta cccggtccct gcccggcctg
    14161 cggaacagag atagaataca tctaccagac agaagagatt ccctactttt ttgagatcct
    14221 catcatatcg gccctgtgcc caacctgcgg gtaccgcctt gcagacacag agcttctgaa
    14281 gaacgccgaa ccgtcgcggt gggaactcac tatcgagtca ccagccgacc ttgatatcag
    14341 ggtggtccgg agcatgaacg gggtcatcag catcccggaa cttggcgtgc acgtcgaccc
    14401 cggtccggcc tgcgagggct ttgtctcaaa cgtcgagggg gtgctgaacc ggatcgacgc
    14461 ggtccttgag agcgtgatcg gctggacaga cgatgatgac gagaggagga acgcattgca
    14521 attgcgcgag cagatcgcag aagtaaagaa aggcaggctc cctgtcacgc tacggataga
    14581 cgacccgacg ggaaactcgg caatccttgg agacagggca aggatctacc ccatcaccgc
    14641 cgccgagtgc gggtatggcc ccgatacggc tgaataatac gttcaacgga tctcttcttc
    14701 ctccaggtat atgccgggac gggcggtgac gacccctgcc acctggcttc ctctcactct
    14761 cttgaggacc gggccttcgg caccctcggg gacgatgcag gcaaagccca ttcccatgtt
    14821 gaacgtccgg tacatctcgg ctctctcgac ctccccatgc tcctggaccc acgagaatat
    14881 ctcctgggga ggcatcgggt ccgtgaactc gaacccgaaa ccggtcagcc gtgagaagtt
    14941 gaggagacct cctccggtaa cgtggcacat cccatgtatc tctgtctctc tcgcgaggtc
    15001 gagcacctcg tggtagatgc gggtgggcac taaaagttcc ctccctatgc tcctgccgct
    15061 cgagagcgtc tcgtcccatg cgtcgtggct ctccactacc cggcgggcaa gggagagtcc
    15121 gttgctgtgg ataccggaag aggggagccc caccaccagg tccccggggc ggatcttctc
    15181 gccagcaatc actctctcct tcctctggac cccgaggcac gtccctgcaa ggtcaaggcc
    15241 attcacgagc cccttgagag tggcggtctc cccgccgacg atattcacat ttgcctgccg
    15301 agccccctcg tttaagccct cgcctatctg tgccatctgc ccgacagaga ggcgatcggt
    15361 ggcgatgtag tcgacgaatg ccaccggctc catgttcatc acgtagaggt cgttgacatt
    15421 catggccatg cagtcgattc ccactgtcga ccagtcctgg agacggtcag ccaccagcat
    15481 cttcgtgcca acgccgtcaa ccgtgagggc gagcgcaatg tcgccgaagt ctatcagccc
    15541 ggcaaagtgg ccgacgcccc ccatcatggg atacgctccc cttcgcctga acgagaggtt
    15601 ctgcacgagt gcagagatgg cgcgtgcctc aaggccgata tcaaccccgg catcccggta
    15661 ggtgcgtggg ctactcattg tcctcctctg aaaggtggaa ctcctcatgg aggatgcgca
    15721 tggccctggg tccgtcctcc tgccttacca cgaacgagat gttgacttcg gaagagccct
    15781 gggagatcat cattacgttg atcccgccat tcccgagcgc agtgaatatc ctgccgccag
    15841 tcccctttgc cccggccatt cccgaaccca ctactgccac tgcgcagacg tccctgttgg
    15901 aggtcacctg cctgaccatc ccctgcttaa cgagggcaag aagggcctcc ctcgccgtct
    15961 cttcctggct ctcctccaca acgagggtga tgtttgcttc agatgatccc tgggagatca
    16021 tcatcacgtt cacgtccctg tcggcaagct ggctgaatat cgccctggca acaccaggcc
    16081 tcccgatcat ctgggccccg cagatgttga ccatcgccac cttctcgatg taggtgaggg
    16141 ctttcactac gcggctgtcc ctcttctcgc ccctgacgat cgcggttccc gggtgatcgg
    16201 ggttgaaggt attcttcaca cggacgagga tgttcttgtt cattgccggc tcgatggacc
    16261 ggggatgcat cacctttgcg ccgaaatacg agagctccat cacctccagg tacgagatgg
    16321 aagagatcac cctggcatca gggatcagcc tcgggtcgga tgtcatgatg ccgtccacgt
    16381 ccgtccatat caggatctcg tctgcattga tggctgcgcc gatgattgca gccgagtaat
    16441 ccgagccgga tcgtcccagg gtggtgacga cgcccttatc cgtgcacccc atgaagccca
    16501 tgatgacggg gaccgagtcc gcaagaagcg ggaggaccct gctctggatc tttccctcac
    16561 tcatgggaag tgccatggcc tcgccgtggt tcgccgtggt gactatgccg gcttcacacc
    16621 cgtccagtgt catagagggg attccacgct gtcggaatgc ggcagatacc accaagctgt
    16681 tgagcctctc gccaaacgtg atgatgtagt cccttgatcg gggagtcagc tccctcagat
    16741 tatgcaccgc gttgaggatg ttctcgaggt tgcagagctg ctcctccagc aggatgccca
    16801 cctcgtcggc gtaatcctct gccgaatcga ggaggacctt tccgtgccgg attcgcaagg
    16861 agttgatgaa cgattcgatc ccgctggaat cggtgctatt ggccagttcg gtggccatgg
    16921 tgatcagctg gtcggtgaca ccccgctggg ccgagaccac caccgcaacc tcgtttcccg
    16981 cggcataaca ggattgcaca atatccacaa cccgggagac actcgtcccg tctgcaacgg
    17041 aagtgccgcc aaatttcatc aaaaacctca tttgggctca cgctgtcctt tacaagctat
    17101 ttatgtacgt atatgaccca ccttataata agatgcggtc agctgaatta ttggactgcc
    17161 atgtcctggt catcgggagt ggcggggccg gggtccgggc tgccatcgag gcctccggct
    17221 acggggagac tgttctcgtc tcaaagaccc tcaccggaaa aggtgggtgc acgacaatgg
    17281 ccgagggagg ctacaacgcg gtgctcaggg agcaggactc ctgcgagatc cacttcgagg
    17341 acacgatgaa gggtggtgcg tacctgaacg accctgatct cgtacgaatc ctggtggacg
    17401 aggcaccgaa gaggatcgat gacctcctgc agtggggagc ggtctttgac gtgaccgact
    17461 gctgcgaggt ggcccagaga ccattcgggg ggcagaggtt tccccgcacg tgctatgccg
    17521 gagaccgcac cggccacgag atgatggcga ccctcgtaga gcggctcgcg ggaacggatg
    17581 tcgacgtgat gaacgaggtt tgcgcgcttg acctcctgaa ggatggcgat agggtacttg
    17641 gggcggtggg gctcaccgag aaggacgagt tggtcgctat ccgtgccgac agcaccgttc
    17701 ttgcaaccgg tggcgggacc aggatttacg acatatccac gaactcctcg agtggaacag
    17761 gcgacggttt cgcaatcggg taccgtgcag gggccgagct gatcgacatg gagatggtcc
    17821 agtttcaccc gacaggcgcc gtgtacccat acgatgcaag aggccggctg atcaccgaag
    17881 cggtaagggg ggaaggtggc gtcctgaaga acagcctcgg cgagcgcttc atgaaccgtt
    17941 atgaccccga gaggatggaa ctctccaccc gcgacgtggt gtcccgtgca atcgcgaccg
    18001 agatccttga ggggcggtgc actggccatg gcggggtcta ccttgacgtg acccacctcc
    18061 cgcggcagca gatcgaggag aagctccctg tcatgctcga gcagttcctc aagttcgggg
    18121 tggacatccg ggaagaaccg atggaggtcg cacccactgc ccaccacatg atggggggcc
    18181 atagaatcac agagaacggg gagacgacca tttccggcct gtatgcatgc ggtgaagtgg
    18241 ccggcggggt ccatggcgcc aaccggctgg gaggaaatgc cctcgcagac acccaggtgt
    18301 ttgggaagag ggctggagag gctgcaggaa agtcaggaaa gaagagtgga cgtatcgatc
    18361 agagggccat ggatgcgcag gaggcaaggc tctccgcgtt ctttgatggc gaggtcgtgc
    18421 cgtccacagt cgcacgatcg ctccggactg ccatgtggga gggggcaggg atattcagga
    18481 ccgcgaagga actcgagaag acactcgggg tgatcaatca cctttcttca ttgcgcttaa
    18541 aggcttccgg gcggtccaac cttgcggaat gctgcatcct gcagaacatg ctcacgactg
    18601 cctcgctggt ggtgaaaggg gcgctgatca ggaaagagtc gcggggtgcc cacgtccgca
    18661 aagacgtgac ccagacgtgg gacccccaga cctctcccta cgggcacagt taccagtcgc
    18721 tcacgcagtc gggaatcgag acgggggtga aggcatgaag aagatgaagg tgaggatcca
    18781 gaggttcgac ccccatcagg acgaaaaacc gcactatcag acgtacctcg tggaggtcaa
    18841 cgagggagca cgtgtcctcc acgtcctcca tgccatccat gacgagatcg atcccacgct
    18901 ctcctaccga tggtgctgcg gttccgggca gtgcggaagc tgcgccgtcc ggatcaacgg
    18961 ggagcctgtg ctggcctgca tggaagaagc cagggacggg atgaccatcg agccgctgaa
    19021 actcccggtg aaaaaggatc tcatcgtcga cctcgagcct gcgcttgagg ctctttcgca
    19081 cctcgagccc ggtgacaagc cggcaatgcc gagcaaggaa gaggtcgaac tcatcaagcc
    19141 gcttcgaagc tgcatcgagt gtctcgcctg cgtctctgta tgccctgcga tggacgtgac
    19201 ctcctttgcc gggccgaccg cgatgcggca gaacctccgc ctcgcgctcg atcccaggga
    19261 ttcaaaggac cgggtgacgg acgcagtctc gaaggggctg tttacctgca cctcatgcca
    19321 ggcctgctgg aaggtatgcc ctaaagacat ccagcttccc ggcaaagcca ttgagaagct
    19381 gcgttccctc gcgaacagga aggggctgac actcccgcgc caccaggagg tcgagaaact
    19441 ggtgagggag accgggagga gcgtcacgcg gaccgagccc accttcatgg agcaggtcgg
    19501 agaggtgatc gagccgtacg gcccggtgaa agccactgtg gggttcttcg tcgggtgcat
    19561 gtacaaccag cggctgccac agaccgcact tgacgcaatg gaagtgctgc gccggaacgg
    19621 cattcgtgtc attatcccaa aggagcaggt ctgctgcgga tcaccgctga tcaggacggg
    19681 ccagtgggac tacctcgata ccctcaagag gaggaacatc gacgcgttcg tattccggaa
    19741 tatcgacact gtgctcacca tgtgcgccgg atgcgggtca accctcaaaa atgactacag
    19801 gactcccttc agggtgctcg acatcaacga gctgctgacc aaatacggga tcgagccccc
    19861 ggcgaggctg cccctgaagg ccacctacca cgacccatgc cacctgatga gggggcaggg
    19921 gatccgggat ctgccgcgtg aactgatcac gcaggtcgtg gatcttgtag agatgccggc
    19981 catctgctgc gggtctggcg gtggcgtaag atcgggagtt cccgaggagg ccgcagcgct
    20041 cgcagagaag c
//
LOCUS       DAOQYR010000035        19951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_3073_length_19951_cov_9.286037, whole genome shotgun sequence.
ACCESSION   DAOQYR010000035 DAOQYR010000000
VERSION     DAOQYR010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 19951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19951
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3073_length_19951_cov_9.286037"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..1599)
                     /locus_tag="PKM87_05820"
     CDS             complement(190..1599)
                     /locus_tag="PKM87_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29619.1"
                     /translation="MPELFMRMMKEEWRMHSTIFGQVSFALFPVMIFGISFMGSFLLP
                     YLRPALLIGDLALATHAMFLVLGAMVGGFGLMGNEMMNRRFGQASLLAYSARSLPLSG
                     KFIFANFVVKDTVYYFFLWVLPFCAGYIAAAPFTGTSLPSAFLLLLTLALAFMAGLCT
                     VFLLSSLYVRSRAACGILVIVGGLALLASFLSTGPTLALLFPPYMLYSGFSWGLLFAL
                     CAILAAMCALSILLFSAEETGTTKVHGDLFTPLVRRFANLQQSPLVAKDLVDLYRSGI
                     GIGQALFSFTIPLVVIWFFLSLAGSFLPSYGLIVLFAMITGVIGSTMYTWVTEFDSFG
                     PYACLPVSVSTLIQSKMTTCALLQVLPAAFIAIAAVLSGESGNLLPAVVLCVAFSLYA
                     AGVMAYLCGLSPSVLVYDVKVLFTYLVLDGVVFAIFSAVSFANPMYAISSVLLLIPGW
                     LLVKAAMRRWDAVDPAGFG"
     gene            complement(1592..2317)
                     /locus_tag="PKM87_05825"
     CDS             complement(1592..2317)
                     /locus_tag="PKM87_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ29620.1"
                     /translation="MAAVLEVSDLSKVYRDVIALDRVSLSVEEGSLFGLLGPNGSGKT
                     TMIKVLTGQIRPTGGSARVLGLDAEHEGLRIRERVGIIPEQESPPSFLTAMEYLEFVG
                     AVRKIPDIRERADEWFDYLEFGDKKNVLCKDLSRGTRQKLMFAQAFIHEPALALIDEP
                     LINFDPIMQDVVKAYLAEYVKSGKTVFLSTHILEVAEEICSSFGILHRGRLLHSGPVT
                     DLTEKGMHLPGFFLSLVRKGGHA"
     gene            2499..3119
                     /locus_tag="PKM87_05830"
     CDS             2499..3119
                     /locus_tag="PKM87_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29621.1"
                     /translation="MNLSRNTALAIFAVVVLIVIGVYVLSGETLISGGDTHSSTGGQE
                     PLQPTPTPLQTPAEGATIMGTTIPTLSPTTRFTTSTTPSPSPIPQKRPVEFTLDSGTP
                     VNCGLTCRETTATITNTGDEVAHSVCVVLEVFNENGEQIFINSAPSIERCLGDIGGGE
                     SRSETITINADCGFLASKCIGHTLILKARATSLERSQAFPDSYIVV"
     gene            complement(3235..4137)
                     /locus_tag="PKM87_05835"
     CDS             complement(3235..4137)
                     /locus_tag="PKM87_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2099 family protein"
                     /protein_id="HNQ29622.1"
                     /translation="MPETRSADAPGRPDRENRGDEHVIEAIGKCRIVIRNGRVTEVGQ
                     PAIRDCPLAKRFACPIPSIDQEAVKANIEHRIASFGMCTPDRVVLETREFVGFGASEI
                     LAFGLDSGLIDAVVLACDGAGTVVVTTPALVQGIGGRMSGLVSTTPYPEVIQRIEECG
                     GIVVDRHHARIDQAAGTGRAYKEGHRRVAATVALPEEAERIRTSYPDAFIFAVHTTGL
                     SREAAEVIVGSSDLVTACASGPIREIAGKKALLQAGIAIPVFAITARGKELVIEKIRQ
                     GREQVLVKTTRLPALGDQQPDPLV"
     gene            complement(4150..4767)
                     /locus_tag="PKM87_05840"
     CDS             complement(4150..4767)
                     /locus_tag="PKM87_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29623.1"
                     /translation="MRPVWYVLLAAVLAGILFSCGCTSQPASSTQAGAATTSVISAVE
                     TSPDMLPVIALHLSDFPEGFELIYAGDTEPPAESTLLADPAYRGGYSVTVSNESSDFP
                     TGELVDQIVLIYSRPVTRERLAEVFTENYPELSTWPLTTLEDPGIGDASIAYHFTYPD
                     TTLSGYTIAFGRKDVYQILTTMTGDGTADYDFLKGIAEKALAKIP"
     gene            5125..9378
                     /locus_tag="PKM87_05845"
     CDS             5125..9378
                     /locus_tag="PKM87_05845"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ29624.1"
                     /translation="MITVLYVDDEPGLLEVSKLYLERHGEFHVETATSARDALELIKK
                     GNYDAVISDYQMPVMNGIDFLKEVRASGNPIPFIIFTGRGREEIVIQALNEGADFYLQ
                     KGGEPGSQFAELAHKVRQAVQQRRAEIRIRDHERREADILNFLPDATFAIDKSGVVIA
                     WNRAIERMTGVKAEHIIGKGNYEYAIPFYNERRPILIDLVRRDDPATAAKYPLIQREG
                     KNLFSEITIPHFNNGLGAALWFTASPLYNTHGEVVGAIESIREITERKKVVDALNESE
                     RRFRELAEMLPQVIYEADADGRVTYANRIAFEMFGYSEEDLRKGVNAMDMVMPDDRNR
                     AEVAFRNVLEKGSPEQQTREYRALRKDGSTFPVSIYSSPVVEDGRIVGVRGIIVDITD
                     RIRAEEKIRESEQNYRLLFETATEGILVAQGDRMVHVNPALIRLLGYPADILTSKPFT
                     DFIHPEDRGKVLNRHLQRMRGEVPHTGYTFRITRGDGEERWVWINSTRIQWSDSPASL
                     SFLTDITEQKKAEQQLISANREYAHLLDQIQDIYYRSDVQGRLTRASQSWARLLGYGD
                     LLECIGRNIADDFYFNPSDRQPLLEEISLNGKVTNFEVMLRKKDGTPVCVETSSHLYY
                     DEEGNILGVEGTFRDITERKEQESILQAELNLGLALQKVRGLHDTLETCLKAAIDVSG
                     MDSGGIYLVDRGTGAVNLITSINLGDAFVKSVSHYPPTSDTARIIREGTPLFIPYGDL
                     EVSYHTPEAEKEGILSIAVIPITSGGKSISCLNVTSHKDIEIPGNRRVALATIATQIG
                     AAIERIMVEETLAESEQQYRNVVEDQTEFISRFLPDGTHVFVNDAYCRYFGFTRKEIL
                     GHRFKPVIPDEERERVQQFFESLTEENPVGSIEHRIIMPDGSIRWQRWSDRAIFDAGG
                     TLIEYQSVGRDITDIRDTLELLHEQEDWYSGLSQVSPDLIFMVDREDRVVYVNSAAAE
                     MLGKGPDEIIGHGRNSLFPPEVSEKQEYAISRVLETGERFRSEGQMHLGGSPRWFDHL
                     LYPVRDRQDNVTGIFGISRDITEQKKIEEDLRIRSEELDSRHRILTTLLETVPIGIFM
                     VEAPTGKPIIANREATRLLGRGVLPDATEGTLAEVYEAFKAGTCRKYPADEMPIIRGM
                     RGEVSHVDDMIVVRPDNTRAHLEVFGTPVIDRHGNVSASLVSFFDITERKQAEMSMKE
                     INKKIALLSSITRHDVANQVTLLRGLAQIAMEKETDGDISDLLLKIDHAGATIARQIE
                     FTRTYQEIGMHEPAWFSLAAIIDKLQQWGVPLISTCSNAEIYADPMIENVFSNLFDNS
                     MRHGGHVTAISVRCEQNQDHLAIIYEDNGTGIPGEKKEKIFERGFGNHTGLGLFLSRE
                     ILGITGISIIETGVFGKGARFEMHVPTGAFRNLSCSLPG"
     gene            complement(9470..10282)
                     /locus_tag="PKM87_05850"
     CDS             complement(9470..10282)
                     /locus_tag="PKM87_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNQ29625.1"
                     /translation="MNDQNSTSSESTWRCTVCGFLHRGERPPRGCPRCGSPTPEFLPA
                     GERTWLTYDGEPFDVLLVNGSTHRAHNTSYMVDIAERELESRGVSYRRFNLIEYTIDH
                     CWCCYSVKAEYCTYPCRNSQDDMPAFHQMLATAKAVIVASPINWNNMSARLKDFLDRT
                     TCMQNLFHLDRPGLTDGKVIGILISGHEDGAIKTALDISLVFEQMGYILAPFGIAFRT
                     HGSQYNSGTDAEFFRNDEMIKNDVRGVVSNVVELMLLDLESRLKGRLVPVSE"
     gene            10652..12850
                     /gene="katG"
                     /locus_tag="PKM87_05855"
     CDS             10652..12850
                     /gene="katG"
                     /locus_tag="PKM87_05855"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HNQ29626.1"
                     /translation="MTEDTKKPVKGGTRQQATGRSMSVRDWWPHQLDLKVLHQHSPLS
                     NPMDRDFDYAEEFKKLDLAAVKKDLRALMTDSQEWWPADFGHYGPLLIRMAWHSAGTY
                     RTGDGRGGAGSGQQRFPPLSSWPDNANLDKARRLLWPIKQKYGRKISWADLLILAGNV
                     ALESMGFRTFGFAGGRVDAWEPDEVYWGSEAEWLGDARYSGERDLENPLAAVQMGLIY
                     VNPEGPNGNPDPIAAAKDIREVFARMAMNDEETVALIAGGHAFGKTHGAGPASHVGPE
                     PEAAPIEEQGLGWKSSYGTGKGNDTITGGPEVIWTQTPTKWSNNFFRNLFTFEWELEK
                     SSAGAYQWKPKGGAGAGSVPDPHDPATRRAPGMLTTDLALRFDPVYEKISRRFYEHPD
                     QLADAFARAWFKLTHRDMGPRSRYLGPEVPKEELIWQDPIPSVNHPLVDAKDIASLKG
                     KILASGLSVSELVSTAWASASTFRGSDKRGGANGARIRLAPQKDWEVNEPVRLSKVLR
                     TLEGIQREFNSKAPGGKKVSLADLIVLAGCAGVEQAAKNAGHNVTVAFAPGRMDASQD
                     QTDVDSFAVLEPKADGFRNYQKARYAVSAEELLVDRAQLLTLTAPEMTVLFGGMRVLN
                     ANFKKTPHGVFTRRPEALTNDFFVNLLDMSTEWKPVSEDADVFEGRDRKTGELRWTGT
                     RVDLIFGSNAQLRALAEVYASADAEEKCIRDFVAVWTRVMNLDRFDLEWS"
     gene            12886..14010
                     /locus_tag="PKM87_05860"
     CDS             12886..14010
                     /locus_tag="PKM87_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ29627.1"
                     /translation="MDKEEGQLHRRRAEVVKGIFRGEPRLISWNITLRCPLACAHCYV
                     DAGGREADGVLSTKEAFGVIDQVCETGRPIIVLSGGEPLLREDIYEIARYGTEQGLRM
                     VMGSSGYLIDGKTAARLREAGIRAVAISLDSANPAVHDSFRGMPGVWEKAVSAIRNLQ
                     DEGIGVQINMSVMRPDVSEVAGVIDLGVSLGVKDFQVFFPVPTGRARGSDIMNPRVYE
                     GLIRGVLERYRDAPLHIRPTCAPQFRRIADELGIENPSWGRGCIAGITYCRIYANGDV
                     TPCPYLPISAGNVREQSFAEIWHHSEVFSTLRDISRLTGKCGRCHYRSICGGCRARAY
                     QGGGTLSTGWCDGLALPAALHGELCGEDPWCPYDPGEFAE"
     gene            14007..14519
                     /locus_tag="PKM87_05865"
     CDS             14007..14519
                     /locus_tag="PKM87_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HNQ29628.1"
                     /translation="MSGRFIPDKLDIAILQALQEEIPLVSRPWKEISGKIGISEQELL
                     ARLNRIKNAGILKAVSPIIESSECGRGAATLVAMHVPSGRLQEVAAIISGYPEVSHNF
                     RREHYYQVWFTLSGRDEEHIRVLVRDILERTGIGEDDILDLPTVNKLKVDVRFSFHGT
                     KATGGNSGSC"
     gene            14506..14994
                     /locus_tag="PKM87_05870"
     CDS             14506..14994
                     /locus_tag="PKM87_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HNQ29629.1"
                     /translation="MDPVDIRLLSLLEEGLPFVSHPYVEIGRQLQMTESEVLERLKWL
                     REEGVIRKFRARINQRKVGITANALVAWKRGESGNGDVGSLLASYPCVTHCYERLPVP
                     GRWEYIFYTVHHGYSREEVLEEVLALSSRIGVADYLVLFSTEEFKRVPNVRIRENGGI
                     MP"
     gene            14991..16169
                     /locus_tag="PKM87_05875"
     CDS             14991..16169
                     /locus_tag="PKM87_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ29630.1"
                     /translation="MNRITQCIHGRGTVSAVMKHRDRPSGEVPSRYLAFAGMRRPVVF
                     WNITNRCNLGCEHCYSRSGPGLRADDELDTNEGFDLIDDLAGMGVPLILFSGGEPLMR
                     ADIWDLAEHAGEKGIKTALSTNGTLITKEVAEKIRNSGIKYAGISLDGARSATHDRFR
                     NSPGAFDRTIAAFARCREAGVRCGVRITLTKENSHELAELIDLSLAIGASRFCLYWMV
                     PCGRGTDSYHRLQVGSNVVEDALGLLYRRAQEIDPSMMEFLTVDAPQDCIHLIQSMER
                     DGSPDLADAQALLASLNGGCSAGDRVANIDPSGNVYPCQFARSPAFLVGNIRERPFSV
                     MWAEETHPVLHKFRGKKALISGRCRECSYFTLCGGGCRVRAYAETGDISASDPFCFVQ
                     "
     gene            complement(16372..17067)
                     /locus_tag="PKM87_05880"
     CDS             complement(16372..17067)
                     /locus_tag="PKM87_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ29631.1"
                     /translation="MEEDVPGAIKQRDGTTYAIVPRTPSGVVTPDELERIANVARKFK
                     IPVIKMTSGQRMVLVGMKKEEIPSVWKDLGMTVGQATAPCVHYVQACPGTETCKYGVQ
                     DSLGLGLEIERMYQEMNLPAKVKIGVSGCPRCCGESYVRDIGIYGSRKGWTVVFGGNS
                     AGRPRIGNVVAKDLSREEAIDLTRRLLTYYRDHGKQKERTARFVERVGLDAIRSDVLR
                     FAPYIKLDDLSAG"
     gene            complement(17276..17572)
                     /locus_tag="PKM87_05885"
     CDS             complement(17276..17572)
                     /locus_tag="PKM87_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAC family transcription factor"
                     /protein_id="HNQ29632.1"
                     /translation="MSSDKEGNYCSVCGGIPPDEIRIRKILVDGKETGIDKLDFILDD
                     VARLGLSGDAEIIAELLKRVQVFNYIPTKKADLYAQALLKEYRAHLQAGSGATH"
     gene            complement(17569..17919)
                     /locus_tag="PKM87_05890"
     CDS             complement(17569..17919)
                     /locus_tag="PKM87_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNQ29633.1"
                     /translation="MTRESKQDLLGCVFEMKDLVEYQTGAVSSRMIINNKAGSITIFS
                     FDKDEGLSEHTAPYDAVVTILDGECEVWVAGVTHAMKAGQTIIFPAHAPHALSATTRF
                     KMALIMIRGKDVAP"
     gene            complement(17935..18726)
                     /locus_tag="PKM87_05895"
     CDS             complement(17935..18726)
                     /locus_tag="PKM87_05895"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ29634.1"
                     /translation="MPGPGPNTTQLIGIAYALIFILVGTFLWRTGKFSKTVKYLLLLV
                     TLLIGFAFFSPMIPHNFQELVLRSDTFVGFALMGAAIGMGVFFLLAFLFGRHFCGYLC
                     PIGAVQEIAYAAPTPKIRLPWKRALSAIRVIVFILILAAGLGFSISILAFFGIQEFFR
                     LAFSAGFVVFLIIIAISLFVYRPFCRLICPVGAIFQLFASQARWKIRRTDACIECKKC
                     EKVCPTNEAGRDDAKGECYLCGRCIDKCPVEGALRYGGKDTPGNK"
     gene            18797..19603
                     /locus_tag="PKM87_05900"
     CDS             18797..19603
                     /locus_tag="PKM87_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ29635.1"
                     /translation="MEQLIGNLVRLGLKEYEARVYAGLVGIGEGNARQIHEVSRVPRP
                     RVYDILQSLVDRGFVEVRQGTPLSYRPVEPPVVIAHLIEALQRAATESSEALEVLSLS
                     AHQKFSPIWYVQGDWSIRRNLASAIRDARESLYLLVLDPELPVEYADVLHDCPPAITL
                     KVLVARDTAVNVKLPRSEVYLVGEFCPLFREEIFSKIFASEIRKEGSVFRLDGIVLSD
                     DQELMIIYRMNNERMAVVVTLPFITAVQSLFFGQMCRSAEPINPVTHQVV"
     gene            19615..>19951
                     /locus_tag="PKM87_05905"
     CDS             19615..>19951
                     /locus_tag="PKM87_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNQ29636.1"
                     /translation="MKCTKVSETASQPNPHHVDARKVYDSPHAVAVVITLQPGESLKK
                     HATPVDVFFYVLEGTGIVEIGQERETVGKDTLVESPARIPHRWINESRGVFRVLVVKV
                     PRPTEETKLL"
BASE COUNT         4483 a         5569 c         5936 g         3963 t
ORIGIN      
        1 gtaggcgcag gagtggtctg ggtaacggga gaaggctggg tacatccagc gaagaggagg
       61 attgcggcaa gcacgaggag cagtactccg agttttgttc cgggattaat gtccatggaa
      121 gagttctcgg gagtctatca atatagggat tttaatataa tcgccataaa aatgacggga
      181 cggatccctc tacccgaatc cggcagggtc caccgcgtcc caccgcctca tcgcagcctt
      241 cacgagcaac cacccaggga tgaggagcag aacagacgag atggcataca tgggattggc
      301 aaatgacacc gcggagaata ttgcaaacac cacgccgtcg aggacaaggt aggtgaagag
      361 aaccttcaca tcatagacga gcacgctcgg cgagaggcca cagaggtatg ccatcacgcc
      421 tgcggcatac agggagaaag cgacgcagag cacaactgcc ggaaggagat ttcccgactc
      481 tccggacagg actgccgcaa tggcgataaa tgccgcaggc aggacctgga ggagcgcaca
      541 cgtcgtcatc ttgctctgta tcagcgtgct gaccgatacg gggaggcagg catagggtcc
      601 gaacgagtca aattccgtca cccatgtgta catggtcgag ccaatcaccc ccgtgatcat
      661 ggcgaacaga acaatcaggc cgtatgaagg gaggaacgat cccgcaagcg agaggaaaaa
      721 ccagatcacg acgaggggta tggtaaacga gaagagggcc tgcccgatgc cgattccact
      781 ccggtacagg tcgacgaggt cctttgcaac cagcggggat tgctgcagat tcgcgaacct
      841 tctcacgagg ggagtaaaaa ggtccccgtg cactttcgtg gtccctgtct cttccgcgct
      901 gaagagcagg atagagagcg cacacattgc tgcgagtatc gcacacaagg cgaagagcag
      961 gccccacgag aacccggaat agagcatgta gggggggaag aggagtgcga gggtcggccc
     1021 cgtcgagaga aaggatgcaa ggagggccaa tccgccaaca atgaccagaa tcccgcaggc
     1081 tgctctcgat cggacgtaga gggacgagag gagaaagacg gtgcagagcc ctgccatgaa
     1141 tgcaagtgcc agggtgagca ggaggaggaa cgccgatggg agcgatgtcc cggtgaatgg
     1201 cgcggccgcg atatatcccg cgcaaaacgg gaggacccag aggaagaagt agtagaccgt
     1261 gtccttgaca acgaaatttg caaagataaa tttcccggag agaggcaggc tccgggctga
     1321 ataggcgagc aggcttgcct ggccgaaccg ccggttcatc atctcgtttc ccatcaggcc
     1381 gaaccccccg accatggcgc cgagcacgag gaacatcgcg tgggtggcga gtgccaggtc
     1441 accgataagc agggccggcc gtaggtaggg caggaggaag gatcccatga aggagatccc
     1501 aaagatcatg acaggaaaca gcgcgaaaga gacctgcccg aagatcgtgg aatgcatccg
     1561 ccactcctcc ttcatcatcc gcatgaagag ttcaggcatg gccacccttc cggacgagcg
     1621 agaggaagaa cccgggaagg tgcattccct tctcggtcag gtctgtgacg gggcctgaat
     1681 ggaggagtcg tcccctgtgc aggatcccaa atgatgagca tatctcctcg gccacttcga
     1741 ggatgtgagt ggagagaaag actgtctttc ccgacttcac atactctgcc aggtacgcct
     1801 tcaccacgtc ctgcatgatc gggtcgaagt tgatgagggg ctcgtcaatc agggccaggg
     1861 ccggttcatg gatgaaggcc tgggcgaaca tcagcttctg cctggtgccg cgggagaggt
     1921 ccttgcagag cacgtttttc ttgtcgccaa actccaggta gtcgaaccat tcgtctgccc
     1981 gttccctgat gtccgggatc ttcctgactg ccccgacaaa ttccaggtac tccatcgcgg
     2041 tgagaaagct cgggggtgac tcctgctcgg ggatgatccc gactctttcc cggatgcgga
     2101 gcccttcatg ttccgcatca agcccgagga ctcgtgcaga cccgccggtc ggccggatct
     2161 gccccgtcag cacctttatc atggtagttt tcccggaccc gttcgggccg aggaggccga
     2221 agagcgatcc ctcttcgacc gagagggaga cgcgatcgag cgcgatgaca tcgcgataca
     2281 ccttcgagag atccgagact tccagcactg cggccattct ccacctccat ggcatcctgc
     2341 aaggctcaac tgcagtggac gcaaacgtga gagtggttct ccctccatcg caataaaacc
     2401 cgggttttcc ccccgatggt cactggtaag agtgggtacg tgaatacaag ggtggaagat
     2461 gcgaaacttc atgcctgtct ttcacccata tggcgtcaat gaacctctct cgcaataccg
     2521 cgcttgcgat cttcgcggtg gtagttctca tcgtcatcgg ggtatatgtc ctgtccgggg
     2581 agaccctcat ttcaggagga gatacccact cttccaccgg cgggcaggag cccctccagc
     2641 cgacgccaac acccctgcag accccggcag aaggcgccac gatcatggga acaacaatcc
     2701 ctaccctctc cccaacaacc cgcttcacca cctccaccac cccgagccca tcaccgatcc
     2761 cccagaagag gcctgttgaa ttcaccctcg actcggggac ccccgtcaac tgcgggctca
     2821 catgccgcga gacgacggcg acgatcacaa acacggggga cgaggttgcg cattcggtgt
     2881 gcgtggttct cgaggtattc aacgagaacg gggagcagat tttcatcaac tccgccccgt
     2941 ctatcgagcg atgcctggga gatatcgggg gaggagagtc gaggagcgag acgatcacga
     3001 tcaatgcaga ctgcggcttt cttgcgagca agtgcatcgg ccatactctg atccttaagg
     3061 ccagggcaac atccctggag aggagccagg cgtttccgga cagctacatc gtcgtgtgag
     3121 cggaagggaa gagttccgcg agaagggcat cggacccgcg gcggttcgcc aggtttcgcc
     3181 ggatagatgg gaatgtgtga gggaaaatga gaacccccgc ccctgtaccc gttatcagac
     3241 tagcgggtcg ggttgctggt caccgagcgc agggagccgc gtggtcttga cgaggacctg
     3301 ttcgcgtccc tgccggatct tctcgatgac gagctccttt cctctcgcgg tgatggcaaa
     3361 taccgggatg gcaatcccgg cctgcaggag tgcttttttc ccggcaatct cccggatcgg
     3421 cccggatgca catgccgtga caaggtccga tgagccgacg atcacttccg ccgcctctct
     3481 cgaaaggccg gtggtgtgga ccgcaaatat gaacgcatcc gggtaggacg tgcgtatcct
     3541 ctctgcctct tccggcaagg ccaccgtcgc tgcaactctg cgatgaccct ccttatacgc
     3601 acgcccggtg ccggctgcct ggtcaatcct ggcatggtgc ctgtccacga cgataccccc
     3661 gcactcctca atcctttgta tgacctcggg atatggggtg gtcgagacga gccccgacat
     3721 cctcccgcca atcccctgga cgagagcggg ggtggtcacg accacggtcc ccgccccgtc
     3781 gcaggcaagg acgaccgcgt cgatgaggcc tgaatcgagt ccgaacgcga ggatctcgga
     3841 tgcgccgaaa ccgacgaact cccttgtctc cagcacgacc cgatccgggg tgcacatgcc
     3901 gaaggaggcg atccggtgct cgatgttcgc cttcaccgcc tcctggtcga tcgaggggat
     3961 ggggcaggca aaccgcttcg cgagggggca gtccctgatc gcgggctggc cgacttccgt
     4021 gacacgcccg ttccgtatca cgatccggca ttttccaatc gcctcgatga catgctcgtc
     4081 tccccggttc tccctgtccg gccttccggg tgcgtctgcg cttctggttt caggcatgac
     4141 cgttacattt caggggatct ttgcgagggc cttttcggcg atccctttca ggaaatcgta
     4201 gtccgcagtg ccgtcccctg tcatcgtggt gaggatctgg tagacgtctt tcctgccaaa
     4261 ggcgatggta taccctgaga gcgtggtgtc agggtacgtg aagtgatacg caatgctggc
     4321 atcccctatc ccgggatctt caagcgtcgt gaggggccag gtggagagct cggggtagtt
     4381 ctccgtaaag acttcggcga ggcgctcccg ggtcacgggc cgggagtaaa tgaggacaat
     4441 ctgatcgaca agttctcccg tgggaaaatc agaactctcg ttgctcaccg tcaccgagta
     4501 gcccccacgg taggctgggt cggcaaggag cgtgctctct gccgggggtt cggtatctcc
     4561 cgcataaatc agctcgaagc cttcggggaa gtctgacagg tgcagggcaa tgacaggaag
     4621 catgtcaggg gaggtctcca ctgcagagat tacactcgtg gttgccgccc ccgcctgggt
     4681 tgatgatgcc ggctgcgagg tgcacccgca ggagaagaga atccctgcca gcaccgctgc
     4741 caggagtacg taccacacag gtctcattca gaaccacttt gatcagcttg aaatctccca
     4801 cccaatagtg gttttggttc attcctgcac aatgtattgg caggagagca gggtttgctg
     4861 tcccggggtc gagaaggatc tgactgggaa taaagacagg tgggaaataa cggtgaattg
     4921 aaagcaatca agaatcagga tagtttcaaa gtgcaacatt gaatgaaatg ggagtgcagg
     4981 cgcgagatat tgagccgggg aacgggatga ttctccctgt aaggtgtata aattgcactt
     5041 caccagagta tccctttcca gaagaattac caaaagagta actatcaata cactccggaa
     5101 taatgatgca atgtaccaac aaccatgata accgtcctct atgttgatga cgaaccaggg
     5161 cttctcgagg taagtaagct gtacctggaa aggcatggcg aatttcacgt tgagaccgca
     5221 acatctgcga gagatgctct tgagctcatc aaaaaaggaa attacgatgc agtcatctct
     5281 gattaccaga tgccggtgat gaacgggata gatttcttaa aagaggtccg tgcttcgggt
     5341 aatccaattc ccttcatcat ctttaccggg cgtggtcgtg aagagatcgt catccaggcg
     5401 ttgaacgagg gtgctgactt ttatctccag aagggtgggg aacccgggtc tcagtttgcg
     5461 gagcttgcac acaaggtccg gcaggcagtc cagcagaggc gggcggagat acgcatccgg
     5521 gaccatgagc gacgggaggc agatatcctc aatttcctgc ccgacgcgac gtttgccatc
     5581 gacaagagcg gagtggtgat tgcatggaac cgggctatag agaggatgac cggggtgaag
     5641 gccgaacaca tcatcgggaa aggcaactat gaatatgcca ttccttttta caacgagaga
     5701 cgaccgattc tcatcgacct cgtgcgaaga gacgacccgg ctaccgctgc caaatatcca
     5761 ctcatacagc gcgaaggaaa gaacctgttc tccgagatta cgatccccca tttcaacaac
     5821 ggcctcggtg cggccctctg gtttaccgca tcgcctctct ataataccca cggggaggtc
     5881 gtcggcgcca tcgagtcgat ccgcgaaatc accgagagaa aaaaagtggt ggatgctctg
     5941 aacgagagcg aaaggcgctt tcgtgaactt gcggagatgt tacctcaggt aatctacgaa
     6001 gccgatgccg atggcagagt gacgtatgca aaccggattg cgttcgagat gttcgggtat
     6061 tccgaggagg atctccggaa gggagtgaat gccatggaca tggtcatgcc cgatgatcgg
     6121 aatcgggctg aagtcgcgtt tcgcaatgtg ttggaaaaag gatctcctga acagcaaacg
     6181 agggagtaca gggcactccg gaaagacggc agtacgtttc ctgtctctat ctattcctct
     6241 cccgtggtag aggatggcag gattgtcggc gtgaggggaa tcattgtcga tatcaccgat
     6301 cgcataaggg ctgaggagaa gattcgcgag agtgaacaga attaccggct cctctttgag
     6361 actgcgactg aaggcatcct tgtggcgcag ggtgaccgca tggtgcatgt caatcctgcg
     6421 ctgatcaggc ttcttggcta ccctgcagat atcctcactt cgaagccatt cacagatttc
     6481 attcatcccg aagatcgcgg gaaagtactg aaccgccatc tccagcggat gaggggagag
     6541 gtaccccaca caggatacac attcaggata accaggggag acggtgagga aagatgggtc
     6601 tggatcaact cgacacggat tcagtggtca gacagccctg cgtcgctctc tttcctcacc
     6661 gatattaccg agcagaagaa ggccgaacag caactgatct ccgccaaccg ggaatatgca
     6721 cacctgctcg accagatcca ggacatatat tacaggagtg acgtccaggg ccggcttaca
     6781 agggccagcc agtcgtgggc aaggctcctt ggctatggcg atctcctgga atgcattgga
     6841 cggaatatcg cggatgattt ttatttcaat ccttcggacc ggcagcctct cctcgaggaa
     6901 atctccctga atggcaaagt gacaaacttc gaggtaatgc tcaggaagaa ggatggcacc
     6961 cccgtgtgtg tcgagacaag cagccacctc tattacgatg aagaaggcaa catcctcggg
     7021 gtcgagggaa catttcgtga tatcacagag cggaaggaac aggaatccat cctgcaggcc
     7081 gagctcaatc tcggccttgc actccagaag gtgcggggat tgcacgatac gctggagacc
     7141 tgtcttaagg cggcaataga tgtatccggg atggattcag gaggaatcta tctcgttgac
     7201 aggggaacgg gggcagtgaa ccttattact tcaatcaacc taggagacgc gttcgtgaag
     7261 agtgtctctc attacccccc gacgtctgac actgcacgga ttatcaggga gggcactccc
     7321 ctcttcatcc cctacggaga tctggaggtt tcatatcaca cacccgaggc agagaaggag
     7381 ggaatcctgt ccattgcagt aatcccgatc acttcgggtg gaaaaagtat ctcctgtctg
     7441 aatgtcacct cgcataagga tattgagatc cctgggaaca ggcgtgttgc cctcgcgacg
     7501 attgcgacgc agataggggc agcaattgag cggatcatgg ttgaagagac cctcgcggag
     7561 agcgagcagc agtatcggaa cgtggtcgaa gaccagaccg agtttatctc ccggtttcta
     7621 ccggacggca cgcacgtctt tgtcaatgat gcctattgca ggtatttcgg gttcacgcgc
     7681 aaagagatcc tcgggcacag gttcaagccc gtgattcctg acgaggaaag ggagcgtgta
     7741 cagcagttct tcgaatccct gacagaggaa aatcccgtgg gaagtattga acaccggatc
     7801 atcatgccgg atggcagcat acggtggcag agatggagtg accgtgcgat cttcgacgca
     7861 ggaggaacac tcattgaata ccagtcagtg ggcagggata tcactgatat cagggacacc
     7921 ctggagttat tgcacgaaca ggaggactgg tattcaggcc tatcgcaggt atcccctgat
     7981 ctcatcttca tggtcgaccg cgaagaccgg gtcgtatatg taaattccgc cgctgccgaa
     8041 atgctcggga aaggacctga tgagatcatc ggccatggga gaaacagcct gtttcccccg
     8101 gaagtctctg aaaagcagga atatgccatt tcacgtgtgc tcgagacagg ggagcggttc
     8161 aggagtgaag ggcagatgca tctcggaggg tccccgcgct ggttcgacca cctcctttac
     8221 cctgtcaggg acaggcagga caatgtcacg gggatatttg gcatttcaag ggacatcacc
     8281 gaacagaaga agatagagga ggatttacgc atcaggagcg aggagctcga cagccgtcac
     8341 cggatcctca cgacactcct tgagactgtc ccgatcggca tcttcatggt tgaagcgccg
     8401 acgggtaaac ctattatcgc caaccgcgag gcaacccgtc tccttggcag gggtgtcctt
     8461 ccggatgcga ccgagggaac cctcgcggaa gtatacgagg cctttaaggc aggcacgtgt
     8521 agaaaatacc ccgccgacga gatgcccatc atacggggga tgagagggga ggtgagccat
     8581 gtcgacgaca tgatagtcgt gcgccccgat aatacccgtg cgcatctgga agtgtttggc
     8641 actcctgtca ttgaccggca cggcaatgtc tctgcaagcc ttgtcagttt ctttgatatc
     8701 accgagcgca agcaggcgga gatgtcgatg aaggagatca acaagaagat cgcacttctt
     8761 tcgagcatta cacggcatga tgttgcaaac caggtgaccc tcctccgggg gcttgcacag
     8821 atcgcgatgg agaaagaaac cgatggggat atctccgatc tccttctgaa aattgaccac
     8881 gcaggggcca cgattgcaag acaaatcgag tttacccgga cctaccagga gatcgggatg
     8941 cacgaacccg cctggttttc ccttgccgcc atcatagata aactgcagca atggggggtc
     9001 cctctcatca gcacgtgttc aaacgccgaa atatatgcag atcccatgat tgaaaatgtc
     9061 ttctcaaatc tcttcgataa ctccatgcgg catgggggtc atgtgacggc tatctcggta
     9121 aggtgcgaac agaatcagga tcaccttgcc atcatctacg aggacaacgg gacaggcatc
     9181 cccggagaga agaaggaaaa aatattcgag cgcgggtttg ggaaccacac cggacttggg
     9241 ctcttcctgt cacgggagat cctcgggatt accgggattt ccatcattga gacgggtgtc
     9301 tttggcaaag gggcacggtt cgagatgcat gtcccgacag gagcgtttcg gaatctctcc
     9361 tgttccctgc ccggctgagg ggaataccag ggagatacct cttggataat taaaaaatct
     9421 gattactcct tcaaatcctc tcgaattctg tccacattct ctctctggat cactccgaca
     9481 ccggcaccag cctccctttc aggcgcgact ccaggtcaag cagcatcagc tcgaccacgt
     9541 tcgagacgac tccccgtaca tcgttcttta tcatttcgtc gtttctgaag aattccgcat
     9601 cagtgccgct gttatactgc gatccatgcg tcctgaacgc gatcccgaaa ggtgccagga
     9661 tataccccat ctgctcgaat acgagggaga tgtcaagcgc ggtcttgatc gccccgtcct
     9721 cgtggccgga gatgaggatc ccgatgacct tcccgtcggt cagaccgggc ctgtccaggt
     9781 ggaagaggtt ctgcatgcag gtggtcctgt cgaggaaatc cttgagccgg gcggacatgt
     9841 tgttccagtt gatgggagag gcaacgatga ctgccttggc ggtcgcaagc atctggtgaa
     9901 atgcgggcat gtcgtcctgg gagttcctgc agggatacgt gcagtactcc gccttcacgc
     9961 tgtagcagca ccagcagtgg tcgattgtgt actcgatcag gttgaacctc cggtagctca
    10021 cgcccctcga ttcgagttct ctctctgcaa tgtcgaccat gtacgaggtg ttgtgggccc
    10081 gatgggtgct cccgttgaca aggaggacgt cgaatggttc gccgtcgtac gtgagccagg
    10141 tgcgttctcc tgccggtagg aattccggtg tcgggctccc gcacctgggg cacccccttg
    10201 gcgggcgctc gcccctgtgg agaaatccgc agacagtgca tctccatgtg ctctccgaag
    10261 aagtgctatt ttggtcgttc atcgcgggtg cggtgtcacg catgttcttt cacggtctcc
    10321 cattatgcag tggagggagt tggaattgag atgggatata ggttgagact ctgggatggt
    10381 gcgcccggtc acctgtcctg actttccgtg aaccaaatgc ttaaagtgcc ggtgcctgaa
    10441 ttactcacgt gaggaatgga tgtggtgaag aataccttca ccattcacca gagggggttt
    10501 taaactggga ttaccaaaac acccgtacct cgggccagaa gggccctgtt ctccttcagc
    10561 cttgaatacg gcaactgtga accagatgca cagcggagga tttcacgccc cacagtgaaa
    10621 tgacccatac aacaagagga gagacgatgt gatgactgaa gacacaaaga aaccagtaaa
    10681 gggaggcaca cgccaacagg cgaccggaag atccatgtcc gtccgggact ggtggcctca
    10741 ccagttggac ctgaaggttc tccaccagca ctctcccctg tccaacccga tggacaggga
    10801 ttttgactat gccgaggaat tcaaaaaact tgacctggcc gccgtgaaga aggatcttcg
    10861 ggcgctgatg actgattcgc aggagtggtg gccggcggac tttggccact acggtcccct
    10921 gctcatccgc atggcatggc acagcgccgg cacataccgc accggtgacg gtcgcggtgg
    10981 cgccggttca ggccagcagc gctttccgcc actgagcagc tggcctgaca acgcgaacct
    11041 cgacaaggcg aggcggctgc tctggccgat caagcagaag tatggcagga agatttcatg
    11101 ggctgatctc ctgatccttg cgggaaacgt cgcccttgag tcgatggggt tcaggacgtt
    11161 tggctttgcc ggcggccgcg tggatgcctg ggagcccgat gaggtctact ggggatctga
    11221 agccgagtgg ctcggcgatg cacgttattc gggcgagcga gaccttgaaa acccgctcgc
    11281 tgccgttcag atgggactca tctacgtcaa cccggaaggc ccaaacggca acccggaccc
    11341 gatcgcggct gcgaaggata tccgcgaggt cttcgcccgc atggcgatga acgacgaaga
    11401 gacggttgcg ctcatcgccg gcggtcacgc gtttggcaag acccacggtg ccggccccgc
    11461 atcccacgtg gggccggagc ccgaagcggc ccccatcgag gagcaggggc ttgggtggaa
    11521 gagcagttac gggaccggca aaggcaacga cacgattacc ggtggcccgg aagttatctg
    11581 gacccagacg ccgacgaagt ggagcaacaa cttcttccgg aacctgttta cgtttgaatg
    11641 ggaactggaa aagagctctg ccggtgcgta ccagtggaaa ccgaagggcg gcgcgggcgc
    11701 aggcagcgtg ccggatcccc atgacccggc aacgcgtcgc gctcccggca tgcttaccac
    11761 cgaccttgcc ctgcggttcg atcccgtcta tgagaagatc tcgaggcgct tctacgagca
    11821 cccggaccag cttgcggacg cattcgcccg ggcgtggttc aagctgacgc accgcgacat
    11881 gggcccgcgt tcacgctatc ttggccctga ggttccaaaa gaagagctca tctggcagga
    11941 ccccatcccc tcagtcaatc accccctggt tgatgcaaag gacatcgcct cactcaaggg
    12001 caagatcctg gcttccggcc tgtcggtctc ggagctggtc tcgaccgcct gggcgtcggc
    12061 atctaccttc cgcggctccg acaaacgcgg aggtgcgaac ggcgcacgga ttcgtcttgc
    12121 gccgcaaaaa gattgggaag tcaacgaacc tgtccggctc tcgaaggtac tcagaaccct
    12181 ggagggaatc cagcgcgagt tcaacagcaa agcacctgga gggaaaaagg tctcgctcgc
    12241 ggacctgatc gtccttgcgg gttgtgcagg cgtcgagcag gcggcaaaga acgccggcca
    12301 caatgtgacg gtcgcctttg ctccgggaag gatggatgcc tcgcaggacc agaccgatgt
    12361 tgattccttc gccgtgctcg agccgaaggc ggacggcttc cgcaactatc agaaagcacg
    12421 ctatgcggta tcagccgagg aactgctggt cgacagggca cagttgctga ctttaaccgc
    12481 accggagatg acggtgctct tcggcggaat gcgcgtgctg aacgccaact tcaaaaagac
    12541 ccctcacggc gtcttcacca ggcgcccgga ggcgctcacc aacgacttct tcgtaaacct
    12601 gctcgacatg agcacggaat ggaagccggt atcagaagac gccgatgtgt tcgaaggccg
    12661 cgatcgcaag accggggaac tccggtggac cggcacgcgt gttgacctga tctttggctc
    12721 gaatgcccag ctccgtgccc tggccgaggt ctacgcaagt gcggacgcgg aagagaagtg
    12781 tatccgggac ttcgtggcgg tctggaccag ggtgatgaac ctggatcgtt tcgatctcga
    12841 gtggtcgtaa tcttccacgc ctttgatgat ggagaacccc aaatagtgga taaggaagaa
    12901 ggacagttgc atcgaagaag ggctgaggtg gtgaagggaa tatttcgcgg ggagccacgg
    12961 ctcatctcct ggaacatcac gctcaggtgc cccctcgcgt gcgcccactg ctacgtggac
    13021 gccggcggcc gcgaggcaga tggagtactc tccaccaaag aggcattcgg cgtcatcgac
    13081 caggtctgcg agacaggaag gccgattatt gtgctgagtg ggggagaacc cctcctccgc
    13141 gaggatatct acgagatcgc ccggtatggg acagagcagg gactgcggat ggtgatgggg
    13201 tcgagcgggt atctcatcga cgggaagacc gcggcacgac ttcgggaagc cgggatacgt
    13261 gccgttgcca taagccttga ctctgcaaat cccgccgtac atgattcgtt ccgcgggatg
    13321 cccggcgtat gggagaaggc agtcagcgcg atccgcaacc ttcaggacga ggggataggt
    13381 gtccagatca acatgtcggt catgcgcccg gatgtgagcg aggtcgcggg agtaatcgat
    13441 ctcggggtgt cgctcggtgt gaaggatttc caggtattct tccctgtccc caccggccgg
    13501 gcaaggggaa gcgatatcat gaaccctcgc gtgtacgaag gcctgatcag gggtgtgctg
    13561 gagagataca gggatgcccc gttgcatatc cgcccgacat gcgccccgca gttccgccgc
    13621 atcgcagatg aactcggaat agagaacccc tcatggggga gaggctgcat cgcgggcatc
    13681 acctattgcc ggatttacgc aaacggtgac gtgaccccct gcccgtatct cccgatcagt
    13741 gcaggaaacg tgagagagca atcttttgct gaaatatggc atcattcaga ggttttttcg
    13801 acactccgtg acatcagccg gctcacgggc aaatgcggga ggtgtcatta caggtcgata
    13861 tgcggggggt gcagggccag ggcgtaccag ggaggcggga ccctctccac gggatggtgc
    13921 gacggccttg ccctccccgc ggcgctccat ggagaactgt gtggcgaaga cccgtggtgc
    13981 ccatatgatc cgggggagtt cgcggaatga gtgggaggtt catacccgat aaattggaca
    14041 tcgcaatcct gcaggccctc caggaggaaa tcccgctggt ttcccgccca tggaaggaga
    14101 tctccgggaa gatcggcatt tccgagcagg aacttctcgc caggctgaac cgcatcaaga
    14161 atgccggcat cctcaaggcg gtctccccca tcatagaatc cagtgaatgc ggacgtggtg
    14221 ccgcaactct cgttgccatg catgtcccct ccggcaggct gcaggaggtt gccgccatca
    14281 tcagcgggta ccccgaggtc tcccacaatt tccggagaga gcattactac caggtctggt
    14341 ttaccctctc cgggagagac gaggagcata tcagggtcct tgtcagggat atccttgaac
    14401 gcacgggaat aggagaagat gatattctgg atctccctac ggtgaacaaa ctgaaggtgg
    14461 atgtccggtt ctcttttcac gggaccaaag cgacgggagg aaacagtgga tcctgttgac
    14521 atcaggctgc tttccctcct ggaagaaggc ctccctttcg tcagccatcc ctacgtcgag
    14581 atcggaagac agttgcagat gaccgagagc gaagttttgg agaggctcaa atggctcagg
    14641 gaagagggag tgatacggaa gttccgggcc cgcatcaacc agagaaaggt aggcatcacg
    14701 gcaaacgccc tcgtggcgtg gaaacgcggg gaatccggca atggtgatgt gggttccctt
    14761 ctggcatcgt atccttgcgt gacccattgc tacgaacgac tcccggtccc cggcaggtgg
    14821 gagtacattt tctacaccgt gcaccacgga tacagccgcg aggaagttct tgaagaggtt
    14881 ttggctcttt cgtcccgtat cggggtggct gactacctcg tgctgttcag caccgaggaa
    14941 tttaaaaggg tcccgaatgt ccggatccgg gagaacggag gcatcatgcc atgaacagga
    15001 tcacccagtg catccacggg aggggcacgg tgagtgccgt gatgaagcac agggacaggc
    15061 cttcaggcga agtgccgagc aggtacctcg catttgcggg tatgcgaagg ccggtcgtgt
    15121 tctggaacat cacgaaccgc tgcaatctcg ggtgtgagca ctgctatagc cgatcgggtc
    15181 ctggcttgcg ggctgatgat gaactggaca cgaatgaagg attcgatctc atcgacgacc
    15241 ttgcagggat gggagtgccc ctgattctct tttcgggagg ggagcccctg atgagggcgg
    15301 atatctggga cctcgcggag cacgcgggtg agaagggaat aaaaactgcg ctcagcacca
    15361 acgggactct tatcacaaag gaagtagcgg agaagatcag gaattccggg ataaaatacg
    15421 cggggatctc gcttgatggt gcacggtccg cgacccatga ccggttcagg aactcccccg
    15481 gcgcattcga tcggactatc gcggcttttg cacgctgcag ggaggctggt gtccggtgcg
    15541 gggtgagaat cacgctcacg aaagagaact cccacgagct cgctgaactg atcgatctct
    15601 cccttgccat cggggcgtcc cgtttctgcc tttactggat ggtgccatgc ggaaggggga
    15661 ccgattcgta tcaccggctc caggtgggaa gtaacgttgt ggaggatgcc ctcggacttc
    15721 tctaccggag agcacaggag atcgatccct ccatgatgga attccttacc gtcgatgctc
    15781 ctcaggactg tattcatctc atccagtcaa tggagcgaga cggatcgcct gaccttgccg
    15841 atgcccaggc cctgctcgct tcactgaatg gcgggtgcag cgcaggcgac agggtcgcca
    15901 atatcgaccc ttctggaaac gtgtacccct gccagttcgc acggtcgccc gcatttttgg
    15961 ttggaaacat ccgggaacgg ccgttctccg tgatgtgggc ggaagagacc cacccggtcc
    16021 tccacaaatt ccgcggaaaa aaggccctta tctcaggacg atgcagggag tgcagttatt
    16081 tcaccctttg cgggggaggg tgccgggtgc gggcatacgc ggagacagga gatatctctg
    16141 catcggaccc gttctgcttc gtgcagtgaa ggcgcggagc ggcaggaaac cgggaccaag
    16201 ggggtccgtg tactttgaaa gcctgccctg cattcctttc atctttcttt aggagggacg
    16261 acccgggagc cggttgtgca ctcattgcca ggacgcacca ggagagatcc cattaagcgg
    16321 ggcccctcac gccagttttc ggtccggacc gtaccgggat gtgtccatac attatcccgc
    16381 ggagagatca tccagtttta tatacggggc aaaccgaagc acgtcggatc tgatcgcatc
    16441 cagccccacc cgttcgacga accgggcagt ccgctccttt tgttttccat ggtcccggta
    16501 gtaggtcagc agcctccgcg tgaggtcgat cgcctcctcc ctcgagaggt cctttgccac
    16561 gacgttccct atcctcggcc tgccggcgct gttccccccg aaaacgaccg tccagccctt
    16621 cctgctcccg tatatcccga tgtcccggac ataactctcg ccgcagcagc gggggcaccc
    16681 ggagacgcct atcttcactt ttgccgggag gttcatttcc tggtacatcc tctctatctc
    16741 gaggccgagt ccgagcgagt cctgcacccc gtatttacag gtctcggtgc ccgggcatgc
    16801 ctggacatag tggacacagg gggcagtcgc ctgcccgacc gtcatcccaa ggtccttcca
    16861 gacagaaggg atctcctctt ttttcatccc gacgagcacc attctctggc ccgaggtcat
    16921 cttgatcact gggatcttga atttcctggc aacattcgcg atccgctcga gttcatctgg
    16981 cgtcaccact ccggaaggtg tgcgggggac tattgcatag gtagttccat ctcgctgttt
    17041 gattgcacct ggtacatctt cttccatgca tgagaattgc ggccgggaag gaataaacat
    17101 atagtcactg gcagagtaac aacattcccg gtgaggattg ggcagaggga ggtcggcacg
    17161 cccggcctca ctgtccccgg acggatattc ccgctacttg tagcctggaa catccgcctg
    17221 gacatgcaga aaaagaaatc acggggattg cccggttcgg atccccggag aattgtcaat
    17281 gggtggcgcc ggatccagcc tgaaggtggg cccggtactc cttcaggagc gcctgggcgt
    17341 agaggtcggc ctttttcgtc gggatgtagt tgaacacctg cacacgcttc aggagctcgg
    17401 cgatgatctc ggcatctccc gagaggccga gcctggccac gtcatcgagg atgaagtcca
    17461 gcttgtcgat gccagtctcc ttcccgtcca cgagtatttt ccggatcctg atctcgtcag
    17521 gggggattcc gccgcacacc gagcagtaat tcccctcctt gtcgctgctc atggagccac
    17581 atctttcccg cggatcatga tgagcgccat cttgaaccgg gtggtagccg agagcgcgtg
    17641 gggggcgtga gcgggaaaaa tgatggtctg ccccgctttc atcgcgtggg tcacccctgc
    17701 gacccacacc tcgcactcac cgtcgaggat ggtcaccacc gcgtcgtagg gtgcggtatg
    17761 ctccgagagc ccctcgtcct tgtcgaacga aaagatggtg atgctcccag ccttgttgtt
    17821 gatgatcatc ctgctcgaaa cggctcctgt ctggtactca acgaggtcct tcatctcgaa
    17881 gacgcatcca agcaaatcct gtttactttc acgtgtcatg gcgatcgctt cctgtcactt
    17941 gtttcctggg gtgtctttcc cgccgtaccg gagtgccccc tcgaccggac atttatcgat
    18001 gcatctcccg cagaggtaac attcgccctt tgcatcatca cgccccgcct cgtttgtagg
    18061 gcaaactttc tcacactttt tacactcgat acaggcatcc gtccgccgta ttttccaccg
    18121 tgcctggctg gcgaaaagct ggaagatagc tccgaccggg cagataaggc gacaaaatgg
    18181 ccggtagaca aagagggaga ttgcgattat aatcaggaag acgacaaatc ctgccgagaa
    18241 cgcaagcctg aagaactcct ggatgccgaa gaacgcgagg atagagatcg agaacccgag
    18301 acccgcagca aggatgagga tgaatacaat cacccttatt gcagagagcg cacgcttcca
    18361 ggggagcctg atcttcgggg tgggggccgc atacgcaatc tcctggaccg cccctatcgg
    18421 gcagagatac ccgcagaagt gcctcccgaa gaggaaagcc aggaggaaga agacacccat
    18481 tccgatcgcg gcacccatga gggcaaaccc gacaaaagtg tctgagcgga gcaccagctc
    18541 ctggaagtta tggggtatca tgggagaaaa gaatgcaaaa ccaatgagga gtgtcaccag
    18601 gaggagaagg tacttcacgg tcttcgaaaa cttccccgtc cgccagagga atgtcccgac
    18661 gaggatgaat atgagagcat acgcgattcc aatcaactgt gtggtattcg gacccggccc
    18721 gggcatgggt tcacctctct accagacgat agcaccggcc atggtattaa catgctgtca
    18781 ctcccagagt aacatcatgg agcaactgat cggaaacctc gtacggctgg gtctcaaaga
    18841 gtacgaggca cgtgtgtacg caggcctcgt cgggataggg gaagggaacg cccgccagat
    18901 acacgaggtg agcagggtcc cgaggccacg ggtctatgat atcctccagt ccctcgtgga
    18961 ccgcggcttt gtagaagtcc gccagggaac gcccctctct taccggccgg tagagccccc
    19021 ggtggtcatc gcacacctta tcgaagccct gcaaagggcg gccacggaga gcagcgaggc
    19081 gctcgaggtg ctctccctct cggcacacca gaagttctcc cccatctggt acgtgcaggg
    19141 ggactggagc atcaggcgga acctcgcatc tgcgataagg gacgcccgcg aatcgctcta
    19201 tctcctcgtg cttgaccccg aactccccgt tgaatatgcc gatgtgctcc atgactgtcc
    19261 tccggcgatt accctgaagg ttctcgtcgc gagggatacg gccgtgaacg tcaagttgcc
    19321 cagatcggag gtctatcttg tcggcgagtt ctgcccgctc ttcagggagg agatcttctc
    19381 gaagatcttc gcaagcgaga tcaggaagga gggatccgtc ttcaggctcg acggcatcgt
    19441 cctctccgac gatcaggagc tgatgatcat ctacaggatg aacaacgaga ggatggcagt
    19501 cgtggtcact ctcccgttca tcaccgcggt ccagagcctc ttctttggac agatgtgccg
    19561 gtctgcggaa ccgataaatc ctgtgacgca ccaggtggtg tgaggaacac cgtcatgaaa
    19621 tgcacgaagg tcagcgaaac tgcctcacag cccaaccctc accacgtgga cgcccggaag
    19681 gtctacgact ccccgcacgc agtggcggtg gtgatcaccc tccagccggg cgaatcccta
    19741 aaaaagcacg caaccccggt ggatgtcttc ttctacgtac tggaggggac gggcatcgtc
    19801 gagatcggcc aggagagaga gaccgtcggg aaggacacgc tcgtggagag cccggcgagg
    19861 atcccccacc ggtggatcaa cgagagccgc ggggtctttc gggtcctggt agtgaaggtg
    19921 ccgcggccga cggaagagac aaaactcctg t
//
LOCUS       DAOQYR010000036        19560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_3152_length_19560_cov_8.642194, whole genome shotgun sequence.
ACCESSION   DAOQYR010000036 DAOQYR010000000
VERSION     DAOQYR010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 19560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19560
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3152_length_19560_cov_8.642194"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PKM87_05910"
     CDS             complement(<1..316)
                     /locus_tag="PKM87_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNQ29637.1"
                     /translation="MTGKVYLVGSGPGGLGLLTLRAREVIDEADVILYDQLPGEEVLA
                     TLPERAEKIDCGKFGGKHTREQDDIEALMIAKAKEGKKVVRLKGGDPFLFGRGGEELE
                     VMR"
     gene            complement(313..1197)
                     /gene="hemC"
                     /locus_tag="PKM87_05915"
     CDS             complement(313..1197)
                     /gene="hemC"
                     /locus_tag="PKM87_05915"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNQ29638.1"
                     /translation="MPLKIGTRGSALALAQADRVISLLEDEGVESKRVIITTHGDTTM
                     GVPLHEIGGQGVFVRALDDAILEGRIDAAVHSMKDIPAKRPAGLATCGILPRDSPADF
                     LVHAQPFQKIKRIGTSSTRRKAQLLMHHPDWRIEPLRGNVDTRIRKLREGAYDAVVLA
                     EAGLQRLGLCLPGTRLAPEMYVPSPNQGTIAVVSREGDDAALLAGLLDHPETRRDVTF
                     ERVVMEEVGGGCFTPQGVYCRERHIRAEILSLDGCRRVRSEGYLSSADDARKFGQLLR
                     RDALDLIQEAYMRLGLKE"
     gene            complement(1182..2429)
                     /locus_tag="PKM87_05920"
     CDS             complement(1182..2429)
                     /locus_tag="PKM87_05920"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNQ29639.1"
                     /translation="MKSSEFFQEAKALMPGGVSSPVRAIRPYPFYTRDASGSRIRTVD
                     GEEMIDCCMAYGPLILGHSHPAIRTAVATQLEKGWLYGTPTPIEPEYASLLVRDHPGL
                     DMIRFVSSGSEATMAAIRLARGFSGKKEIVKVEGGFHGAHDGVLVQAGSGATTLGIPD
                     SAGVLPEVAARTRQVPYNDLDALEDLLSRSRDIAAFILEPVMGNIGPILPDKGYLAGV
                     REITRAHDVLLIFDEVITGYRVGIGGAQVRYGVLPDLTTLGKIIGGGLPIGAFMGRRE
                     IMEMVAPAGPVYNAGTFSGNPLSLAAGKAMIEWLHANQRIYRTLEEEAGMIAEATAGK
                     RCGTMVTLGSMFKFFFRRSPPRNYVEAKECDTAAFRDFWEKMLRQGIFLPPSQFETNF
                     LSAAHSDHDIDAICTAYRACLSK"
     gene            complement(2416..3399)
                     /gene="hemB"
                     /locus_tag="PKM87_05925"
     CDS             complement(2416..3399)
                     /gene="hemB"
                     /locus_tag="PKM87_05925"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNQ29640.1"
                     /translation="MFPQRRMRRLRGRIVQPLLREQVLQKTDLVAPLFIDETASSRRA
                     IPSMPGQFRYPPREAESVARELWDAGIRAVMLFGVPKEKDAQASGAYDPDGVVQRAVR
                     SIKKSVPGMVVITDVCACEYTDHGHCGIVGDTRSGVDLLNDPSLDIMDRIALSHAGSG
                     ADIVAPSCMLDGVVQSMRHALDREGFEDVLIMSYSTKFASALYGPFREAADSGFQFGD
                     RTTYQIHPGNTREAVMESMLDLDEGADILMVKPAGFYLDILAEIRTLGIPVAAFQVSG
                     EYAMIKAAAGEGWIKEKEVVIESLTCIKRAGADLVITYFARDVAGWLDEEQ"
     gene            complement(3378..4661)
                     /gene="hemA"
                     /locus_tag="PKM87_05930"
     CDS             complement(3378..4661)
                     /gene="hemA"
                     /locus_tag="PKM87_05930"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNQ29641.1"
                     /translation="MNMAPSCLLTPLAIAGVSHHTATVDVLEQARYPDEQAFLEKARD
                     RFRGVMLLQTCNRVEIFVHGEAETLAAFLQEEGRGDFSFCTGADAMRHLLELACGIDS
                     MIVGEDQILGQLKKALQQSRDAGCASPVLELCINKAVHAGTEARRRTHINRGAVSIGS
                     AAVQLAEEQLGTLQGKHILVIGSGEMGMLVAQALAAKDLTAIYVANRTYSRAEMLAKK
                     IGGRAVRLDDLPRYMVQSDVVISCTSAPHPVIHCNTLRDVMKARCWPHDKTPRPLILI
                     DIAQPRDVEEGADVIAGISLFSIDNLRRINEMTMHARRSEAEKAREFLDGELLHFVRL
                     LNSRAADDTLAILHTWAEAIRLRERDRALARLGTGDLRAREIIDDLSRVLVKKILSDV
                     TVSIRHCAEMGELESAESLVSAITRGENLCFRNEE"
     gene            complement(4658..5281)
                     /locus_tag="PKM87_05935"
     CDS             complement(4658..5281)
                     /locus_tag="PKM87_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HNQ29642.1"
                     /translation="MIPLLVEFSKKTVVIFGGGEVGARKAAYFAPEARVHVYSRSFSP
                     GFSGLNVKLDTIDLHGAGDERLPALLQGAFLAIAATPDRELNNRIGELCLERGILFNN
                     AEGDAGDLLIPSVVRGERFLLAISTGGSSPAVSRFLREFLEKFLPDLDQMIALQERLR
                     AYLKECQPDANARKEVVWKVLMDRDVWAALHGGEIAAWSLITRRYLQ"
     gene            5541..6608
                     /gene="wtpA"
                     /locus_tag="PKM87_05940"
     CDS             5541..6608
                     /gene="wtpA"
                     /locus_tag="PKM87_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tungstate ABC transporter substrate-binding
                     protein WtpA"
                     /protein_id="HNQ29643.1"
                     /translation="MNRIVKSCVLILIATALVLSCACVTQEQGAPATPAATTAPAGKI
                     TVKVFHAGSLTGPFEKLKAEFETKYPNTEVLLEPGGSVDVIKKVTDLDKPADVVASAD
                     YALIPQYMVPNDADWYLTFAKNQMTIAYTDKSKFASEITADNWYEILGREGVRWAFSD
                     PNADPCGYRTPMVIQLAEDYYKDDTIFDTLVSAHSKITTSRSGNMWTIDATDPSPDGT
                     KLTVRPKSVELVQMLQAGGLDYAWEYRSVSVQNDLKYLELPEAIDLSSVDYAEAYATV
                     QTKAVKGNGTTLYVGAPIVYGVTVPKIAEHPDYGLLFVKMLIGPEGQAILTADGQPPI
                     VPAGGYGTVPSEIAGMVSMKS"
     gene            6716..7552
                     /locus_tag="PKM87_05945"
     CDS             6716..7552
                     /locus_tag="PKM87_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ29644.1"
                     /translation="MADKGKVSSWVRYRRPDSCLLAFTLIGSTLVIFAFLALATITVT
                     QLADLPFLIQVALDSRVIDSILLTFGAGLAVVGVLMILGTPLAYVLARTHFRLNSLIE
                     ALIDIPLVLPHTVAGIMVYLLFMQRGPLGAPFKDIGIVFEDAFPGIVVAMLFVAMPFY
                     INTVREGFQKVPLHIENVARTLGASRFRAFVLVVLPLSTRHILNGCVLAWGRAISEFA
                     AVIMIAYYPMIVSTLIYYRFTTGGLKESSAIAFLMIVASFSVFLVLRIVTRYLGRYDD
                     RV"
     gene            7536..8603
                     /locus_tag="PKM87_05950"
     CDS             7536..8603
                     /locus_tag="PKM87_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ29645.1"
                     /translation="MMIAFDRVSLALGEFALKEISFRVREGDYFFIIGPSGAGKTILL
                     EAIAGLYVPDKGRILLSGRDVSQIPPEKRGVGLVYQDYSLFPHMNVEKNIAFGLKMQG
                     VPAMEMKRTVDNLISRFGIGNLRARSPMTLSGGEMQRVALARALATSPRVLLLDEPLS
                     ALDPSMKEYFIQELREVHQDTGLTIIQVSHDRHEILSLGTRMALIMDGCLEQEGSVRE
                     VYESPVSRRVAEFIGYENVIEGVTISSGDGECLIDAGGMQLRAPFAVPEGRPVTFCIG
                     ADEVVIHPCPVAPVNDENVIPGVVTSLTQAGYLTRAKIDAGITLTAVMIRETAEEMAL
                     KPGSRVSVSIRKKAIRVIVQD"
     gene            8844..8915
                     /locus_tag="PKM87_05955"
     tRNA            8844..8915
                     /locus_tag="PKM87_05955"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8877..8879,aa:Gly,seq:gcc)
     gene            9211..10140
                     /locus_tag="PKM87_05960"
     CDS             9211..10140
                     /locus_tag="PKM87_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ29646.1"
                     /translation="MQEAIEVKSLSKKFGTTVAVDCISFSVKEGEIFGFLGPNGAGKT
                     TTTRMLTGVIPPDTGSATVLSHDISREPVLAKQGFGVVPETSNAYNDLSAWQNLMLVG
                     ELYGVSRDRAASRASRLLGTLGLLQRKDQKVQAYSKGMKQRLILAMALLHEPQLLFLD
                     EPTSGLDVQSTQMILSMLRELNRQGTTIFLTTHNMQEANSLCDRVGIIHAGRMVAVDA
                     PERLKSAIERVHRIEVSFNREVPDGALDALYGVVGVNRTGDKWQISANDQDTAIKSLV
                     EFCRDHNLAILTLNTHAPSLDEVFLQLTQERGP"
     gene            10137..10892
                     /locus_tag="PKM87_05965"
     CDS             10137..10892
                     /locus_tag="PKM87_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ29647.1"
                     /translation="MNPAGLPEQMRRAWAVAKKDIRIYYVKGPVLIFGVFMPVFLFLA
                     FLMGGRQFPLAFLVSGLVGMTLFFTATAVSPAIFPWEGQARTLERLASCPVAVEAIVL
                     GDMAASTIFGIAITLVTVAIGLMLGLTLLNCFLMFGAIILAACCFSAIGMLLAVPPTN
                     VPSNIMMLSSLLKFPLVFISGIFVPLEQLPPWGMTFAVCSPLTYLTDVIRYSFSGSHY
                     FPLFFDIVMLGVFTLLFSGSAMILHKKFLPMRM"
     gene            complement(11036..11968)
                     /locus_tag="PKM87_05970"
     CDS             complement(11036..11968)
                     /locus_tag="PKM87_05970"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNQ29648.1"
                     /translation="MTTAPPDRTHQRYQKERCRGVTRLSKCAIPAVLLVFAVLLSGCI
                     THPPGTGPEESGWNSSPVTPTPIQEGTLPISGITWVLVSYDSGRLAKESIVPGSVVTA
                     RFEREGVMYGSSGCNRYWARYEASPSKVSIDAPQRTNAVCNTPSGVHSQESMYLLDLG
                     KASTYRVDGDFLSLLDGNGRAVLLFGKETAPEDSAHILQETWYLTALRDDQGFIVNSP
                     EGVLVTARFLDETVSGFAGCNCYVAPAYIRSAAIRIETPLATDTWCRNETVRNQERLY
                     LSRIQEVDAYVPGDNSLILVNGQGETLLVFGTRT"
     gene            complement(12050..12649)
                     /locus_tag="PKM87_05975"
     CDS             complement(12050..12649)
                     /locus_tag="PKM87_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29649.1"
                     /translation="MRSHSGPQMILLTCALFMVAAAGCMVPSTSTTTPPGTAAGTGAH
                     QTGDAVFLSLYDTAQSDIESALERMNRHFPVGAATRGAPYLAGELRSSALELRALADT
                     SHTSMIRIESFQEKENEVGRNEYLKYLSAIRKVAGNTADAAAAEMDLQFGLAQNYAES
                     ARNALKDAVETGETEHRDLLRETGVSLDDYILKMREKRV"
     gene            12823..14565
                     /locus_tag="PKM87_05980"
     CDS             12823..14565
                     /locus_tag="PKM87_05980"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HNQ29650.1"
                     /translation="MQVRRTVVYTILILLLAIAAAIFAGMHFMMIPAIAHIESREASN
                     SVLRTVGMVENELGSLEGTAIDWSQWDDTYFFATDRNGEYVKNNLMDKTFTSLQLNLM
                     LYYDLNGTLFYGKGYDYLEFERLEIPQALAVNPAGDILDEIRRNERVEDASIRGIISL
                     PEGLLLLSINPILKSDSTGPAAGYLCIGRYLGDAEVAKIAQLTSTDLSVISAADAGTA
                     WGVMHETLSTQEGSAIVRISPEGNTITSFVVIDDIHGVPAAVFRVDSPRTTHQIAMES
                     LLLSLYLIAAIGCISVLFVIYILDHRFLKRLSLLEKKMKEIADRQDFSARTRLSGDDE
                     LSSLSAKIDHTLDALEEHINAERAAVESARIANEKLTLLSKITRHDILNQVTVIRGFA
                     DLSRDALASDSPAVPFIDRISSASAAIEEQLAFTREYELGGDHAKPAWFNIRDLATGI
                     VRTMPLCEIDVDIDTGDLEIYSDPLLERVIFNLVDNSLGHGEKVTTIRLRFYEEEGSG
                     VLVYEDNGVGIPYHQKEIIFEQGFGRHRGLGLFLAKGVLSVTGITLKETGVPGEGARF
                     EMRVPKGLFRLKQG"
     gene            complement(14628..15950)
                     /locus_tag="PKM87_05985"
     CDS             complement(14628..15950)
                     /locus_tag="PKM87_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ29651.1"
                     /translation="MKKKYCTIVALLLILVLCAACTDQQPVSQETLKIGVVASMTGPA
                     STTGKDIWQSAVLAADEINAKGGVFVKDLNRKIPITLIQGDDESTREGGQKAVSRMIT
                     QDKVDVLVGGFSSAVVSAHQSIVAEHGIPYIITGASTPTITQRTDIDTATMFHYCPTT
                     DDYGEQTTLFVNDAIRDAVNARFKYPDNRPLRLALLVQDSPYGKGVQNAVHDTIERRG
                     LNIVIVAEETFKMGETDFRTVLTTIKAAQPDVVYPAAFLNEQIPMVTQARRDVGLNTI
                     FLAVECNDDPDYYEGLGKYGEYSIIETRFSPYAIPRGDIADDMAAFRENFQKRWGSYP
                     GMMGASTYEGIYIAAQAVENAGTREKVKVRAALDALTMPQMIEAMQGGVITFTDDFRE
                     AKFDLYMEQLVWNETTKELRPVIVWPDHLKEADFVLPEWYVPGPGSSP"
     gene            16029..16757
                     /locus_tag="PKM87_05990"
     CDS             16029..16757
                     /locus_tag="PKM87_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ29652.1"
                     /translation="MLRVNGVTKSFGGLTAVNRVDLSLNKGEILGLVGPNGAGKTTLM
                     NLISGIYRPDSGGIIFGGEDITRLSLDAVCKKGISKTFQHPRSFPGLTTREAVMISAL
                     YGNGRRPSVKEAHSEAEECLCRVGFPEQKIDSPISSLNTIELRRSQLARALASHPKLL
                     LLDELTTGLTPSEGKEAMRLIQEIRSDGLTILIIEHVMRIIMGVSDRIVVLDHGEKIA
                     EGTPEEIACNEQVIDSYLGERYHF"
     gene            16773..17465
                     /locus_tag="PKM87_05995"
     CDS             16773..17465
                     /locus_tag="PKM87_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ29653.1"
                     /translation="MLTIENLNVSYSHLQVLWDVSLKVSRGEIVTLIGSNGAGKSTLV
                     STIFGLNSSPTGRIELKGENILGLPTYEIVRRRLALVPEKRELFPRMTVKENLELGAY
                     VRENGSALDHVLELFPVLKDRQAQNAGTLSGGEQQMLAIGRCLMADPEMIVLDEPSLG
                     LSPKLVHMVLGTVQRLNDEGLTILLVEQNVRRALEISHRAYVLESGRITRTGPADDLL
                     HDDQIREAYLGM"
     gene            complement(17701..18666)
                     /locus_tag="PKM87_06000"
     CDS             complement(17701..18666)
                     /locus_tag="PKM87_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNQ29654.1"
                     /translation="MKIFDPRDYRHLLAAALLVIAFLIPIFIGSNLYYLTILILMLVF
                     IIYASAWNFLTFSGQGSLGHAAFFGLGGYAASLVAIRFGLPPLVTLLFGGCFAAFIGI
                     LIGLTCVRLREWFLAMVTFGFAVIIQTLIVSQLAPITGGWDGFPVPKLVPTTIPGYLM
                     IEYYLILFIAIGVILIFRYLLQSRIGLALAAIRENEVEARAAGINPVPFKLFAFGVSA
                     FVTGIAGGLEIFHFGYITPEIFGIDLSFWPIIYSITGGLGTLAGPVIGTIVVTLLWDG
                     LNAVGLTYARFIIIGVLLVLIIIFLPRGLVSLPEEIQKRIRQKKT"
     gene            complement(18663..>19560)
                     /locus_tag="PKM87_06005"
     CDS             complement(18663..>19560)
                     /locus_tag="PKM87_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNQ29655.1"
                     /translation="EKDYCMDGTIGIISQVAFWGLYSGCIYILLATGLNLIFGVMKIV
                     NFAHGEFLMLGTYITFFLFVISGFNPYVLLIGSIPILIIVGVIVERLCFRPILGTGKL
                     NEIFISLGLIYIFQNLVAMIWTDEWRSMKSPFADILVSLGPLQLPLDYLIIITVTALI
                     LVSLYLFLQKTPLGRAIRAASQNRKGAMLMGVNVERMDMLTFGIGAGLAGAAGTLWAV
                     SGQVFNPYMGSIPAIKGFAIIILGGLGSIPGAIIGGLAIGMVENFTAFTLGGSWKDAV
                     SFILLIVVLVIRPTGLFGEAEE"
BASE COUNT         4081 a         5625 c         5574 g         4280 t
ORIGIN      
        1 ccctcatcac ctcgagctcc tcgccgcccc tcccgaacag gaaggggtct ccgcccttca
       61 accgcacgac cttctttcct tctttggcct ttgcaatcat cagggcctcg atatcgtcct
      121 gctcgcgggt atgcttcccg ccgaacttcc cgcagtcgat cttctcggcc ctctcgggga
      181 gggttgccag gacctcttct ccggggagct ggtcgtagag gattacgtct gcctcgtcaa
      241 tgacttcgcg tgcccgcagg gtgaggagcc cgaggccccc cgggccggaa cccacaagat
      301 atacctttcc cgtcattctt tcagccccag tctcatgtat gcctcctgta tcaggtcaag
      361 cgcatctctc ctgagcagct gcccaaactt gcgggcatcg tctgccgagg agaggtatcc
      421 ctcactcctc actctcctgc acccgtcgag cgagagaatc tctgccctga tatgcctttc
      481 cctgcagtac accccctgtg gggtaaaaca cccgcctccc acctcttcca tcaccacccg
      541 ctcgaaagtc acgtccctcc gggtttccgg gtggtccagc aagccggcaa ggagagcagc
      601 atcgtctccc tccctgctga ccacagcaat tgtcccctgg ttcggggatg gcacgtacat
      661 ctctggagca agcctcgtgc ccgggaggca gagcccgagc ctctggagcc ctgcctcggc
      721 caggacgacg gcatcgtatg ctccttcccg gagtttcctg atgcgggtgt ccacgttccc
      781 gcggagcggc tcgatcctcc agtcagggtg gtgcatgagg agctgcgcct tcctccttgt
      841 actcgatgtc ccgatcctct ttatcttctg gaacggttgt gcatgcacaa ggaaatcggc
      901 gggagagtcg cgcgggagta ttccgcacgt cgcgagccct gcaggcctct ttgcggggat
      961 atccttcatg ctgtgcaccg ctgcatcgat cctcccctcg aggatcgcgt catcgagcgc
     1021 acggacaaag accccctgcc ccccgatctc gtgcagcggg acacccatcg tagtgtcgcc
     1081 gtgcgtggtg atgatcactc tctttgattc gactccctca tcttccagga gggagatgac
     1141 cctgtccgcc tgggcaagtg caagcgcact ccccctggtc cctattttga gaggcatgcg
     1201 cggtatgccg tgcagatggc gtcgatgtca tggtcgctgt gcgccgcgga gaggaagttg
     1261 gtctcgaact gtgacggggg caggaatatc ccctgcctga gcatcttctc ccagaaatcc
     1321 cggaaggcgg cggtatcgca ctcctttgcc tcgacgtagt ttctcggcgg ggagcgtcgg
     1381 aagaagaact tgaacatcga cccgagcgtg accatcgtgc cgcaacgctt ccccgccgtg
     1441 gcctcagcga tcatccctgc ttcctcttcg agcgtgcggt agatccgctg gtttgcatgg
     1501 agccattcga tcattgcctt ccccgcagcg agtgagagag ggttgcccga gaacgtccct
     1561 gcgttgtaga ccggtcctgc gggtgcgacc atctccatga tctccctcct tcccatgaat
     1621 gcaccgatgg gaagaccccc gccgatgatc ttcccgagcg tcgtgaggtc gggcaggacc
     1681 ccgtagcgga cctgcgcccc tccgatcccc accctgtatc cggtgatcac ctcgtcaaag
     1741 atgaggagga catcgtgggc ccgcgtgatc tcgcggaccc ctgcaaggta ccccttgtca
     1801 ggcaggatgg gaccgatgtt tcccatcacg ggctcgagga tgaaggcggc gatgtccctg
     1861 ctccgggaga ggaggtcttc gagcgcgtca aggtcgttgt aggggacctg ccgggtgcgt
     1921 gcggcgactt cggggagcac gcccgccgag tccgggatgc cgagggtggt ggccccggac
     1981 ccggcctgaa ccagcacgcc gtcatgggcg ccgtggaacc caccctctac ctttacgatc
     2041 tccttcttcc ccgagaatcc ccgggccagc cggatcgcag ccattgttgc ctcggagccg
     2101 ctcgacacga accgtatcat gtcgaggccg gggtggtccc tgacaagaag agaggcatac
     2161 tccggttcaa tgggggtggg agtcccgtaa agccacccct tctccagctg tgtcgccacg
     2221 gcagtcctga tcgccgggtg ggaatggccg aggatgaggg ggccgtatgc catgcagcag
     2281 tctatcatct cctccccatc cacggtcctg atcctcgatc cggaagcatc gcgtgtgtag
     2341 aaggggtatg gcctgatcgc ccggacagga ctgctcacgc cccccggcat cagtgccttt
     2401 gcctcctgga aaaattcact gctcttcatc aagccatccc gcaacgtcgc gtgcaaagta
     2461 ggtgatgacg agatccgccc ctgcacgctt gatgcaggtg aggctctcga ttacgacctc
     2521 cttctctttg atccacccct cccctgcggc ggcctttatc atggcatact ccccgctgac
     2581 ctggaacgcg gcgacaggga tgccgagggt tcgtatctct gccaggatat cgaggtagaa
     2641 ccctgccggt ttcaccatga ggatgtccgc cccctcgtca aggtcgagca tcgactccat
     2701 gacagcctcc cgcgtgttcc cgggatggat ctggtaggtg gtccggtccc cgaactggaa
     2761 ccccgagtct gccgcctccc tgaacgggcc gtagagggcg ctcgcgaact tggtcgaata
     2821 cgacatgatc aggacgtctt cgaacccctc cctgtcaagg gcatgccgca tggactggac
     2881 aacgccgtcg agcatgcagg agggtgcaac aatatccgcc ccgctccctg catggctcaa
     2941 tgcgatgcgg tccatgatgt cgagcgatgg gtcgttcaga aggtcgactc ctgaacgggt
     3001 gtcgcccacg atgccgcagt gtccgtgatc ggtatactcg caggcacaga cgtccgtgat
     3061 caccaccatc ccggggaccg acttctttat cgacctgacc gcgcgctgca ccaccccgtc
     3121 aggatcgtac gcaccggatg cctgtgcatc cttctccttc gggactccga agagcatgac
     3181 ggccctgatc ccggcgtccc agagctccct tgcgacagat tccgcctccc gtgggggata
     3241 acggaactgt cccggcattg agggaatcgc cctcctcgat gatgcggtct cgtcgatgaa
     3301 cagcggggca acgaggtctg tcttctgcag tacctgctcc cggaggaggg gctggacgat
     3361 tcgtcccctg agccttctca ttcttcgttg cggaaacaca gattctcacc tctggtgatg
     3421 gcggatacca gcgactcggc agactcgagt tcgcccatct ctgcgcagtg gcggatggag
     3481 actgtcacat ccgagaggat cttcttgacg agcaccctgg agaggtcgtc aatgatctcc
     3541 cttgcccgaa ggtcgcctgt cccaagcctt gcaagcgccc tgtcccgctc cctgaggcgg
     3601 atagcttcag cccaggtatg caggatggcc agggtatcgt cagccgcccg gctgttaagg
     3661 agccttacaa aatggaggag ctcgccatcc aagaactccc ttgccttctc ggcttcactc
     3721 ctcctggcat gcatggtcat ctcgttgatc ctccggaggt tgtcgattga gaagagggag
     3781 attccggcaa tcacatccgc tccctcctcg acgtcccttg gttgcgcgat gtcgatcagg
     3841 atgaggggcc ggggtgtctt gtcatgcggc cagcagcgag ccttcatcac atcgcggagg
     3901 gtattgcagt ggatgaccgg gtgaggtgca gatgtgcagg agatgaccac gtccgactgg
     3961 accatgtagc gggggaggtc gtcgagcctc actgccctgc ccccgatctt ttttgccagc
     4021 atctcggccc gggaataggt ccggtttgcg acatagatgg cggtgaggtc cttcgccgca
     4081 agcgcctgtg caacaagcat gcccatctcc cctgacccga tcacgaggat gtgcttgccc
     4141 tggagcgtgc cgagctgctc ctccgcgagc tgcactgcgg cagaaccgat cgagacggca
     4201 cccctgttga tgtgcgtccg ccttcgtgcc tctgtccctg cgtggactgc cttgttgatg
     4261 cagagctcga gcaccggact tgcgcatcct gcgtccctcg actgctggag ggccttcttg
     4321 agctggccga ggatctggtc ctcacccaca atcatggaat caatcccgca ggcaagctcg
     4381 aggaggtgtc gcatggcgtc tgctcccgtg cagaacgaga aatcccccct cccttcttcc
     4441 tggaggaagg cggccagcgt ctcggcttct ccatggacga atatctccac acggttgcag
     4501 gtctggagga gcatcacccc tcggaaacgg tcacgggctt tctcgaggaa tgcctgctca
     4561 tccggatacc tcgcctgctc gagcacgtcc accgttgcag tgtggtggct gacacccgcg
     4621 atggcaagtg gggtgaggag gcaggagggg gccatgttca ttgcagatac ctcctcgtga
     4681 taagggacca tgcggctatc tcgcccccgt gaagggctgc ccagacatcc ctgtccatca
     4741 gcaccttcca caccacctcc tttcttgcgt tcgcgtcggg ctggcactct tttaagtatg
     4801 cccgaagcct ctcctggagg gcgatcatct ggtcgaggtc tggcagaaac ttctcaagga
     4861 actcgcggag gaaacgagag accgccgggc tcgatccccc cgtgcttata gccaggagaa
     4921 atcgctcacc cctcaccaca gaggggatca gcaggtcgcc ggcatccccc tcggcattgt
     4981 tgaagaggat ccccctctcc aggcacaact ccccgatgcg gttgttcagc tcccgatcag
     5041 gagttgccgc aatggcgaga aacgctccct gaagaagtgc agggagccgc tcgtctccgg
     5101 ctccatgtaa gtcaatggta tccagcttga cattcagccc tgagaaccca ggagagaatg
     5161 acctgctgta gacatgcacc cttgcttccg gagcaaaata tgctgctttc cgtgctccca
     5221 cctcaccgcc tccgaagatc accactgtct ttttggagaa ttccaccagg agggggatca
     5281 ttgctataga tctaccctgt cttttttaat taagatgttg gattcaggat gaaaattgta
     5341 ctgggggaat atcgcatggc attgaattgt ccggtggcgg ggttcggcgt gtgttctcct
     5401 tcactctgcc aggggaggcg agacgttggc acacaccctc gctcgatccc tcaccaactg
     5461 ttaaatcaat tgattatacc aagtaactat ttttactaca attgattagt aatttataga
     5521 ttctcgggta aaagtggtgt atgaatcgaa ttgtgaaaag ttgtgtactc attctcattg
     5581 cgacggctct ggtactctca tgtgcatgtg tcacgcagga acagggagca ccggcaacac
     5641 cggctgcgac cactgccccg gcaggaaaga tcacagtgaa agtctttcat gccggaagcc
     5701 tgacgggccc gttcgagaag ctgaaagccg agtttgaaac taagtacccc aataccgagg
     5761 tcctccttga accgggtggc agtgttgacg tgatcaagaa agtgaccgat ctcgacaagc
     5821 cggcagatgt ggttgcatcg gccgactatg cgttgatccc acagtacatg gtcccgaacg
     5881 acgctgactg gtatctcacg tttgcgaaga accagatgac gatcgcatat acggacaaga
     5941 gcaagttcgc atcggagatt accgcagaca actggtacga gatcctcggc cgcgagggcg
     6001 tcaggtgggc attttccgac cccaacgcgg atccgtgtgg ataccggacc cccatggtca
     6061 tccagctcgc agaggattat tataaggacg acacgatctt tgataccctg gtgagcgccc
     6121 acagcaagat taccacctcc aggagtggca atatgtggac gatcgatgcc accgacccgt
     6181 cacctgacgg caccaagctt accgtgagac ccaagagtgt cgagcttgtc cagatgctcc
     6241 aggcgggagg cctcgattat gcctgggagt accggagcgt ctcggtgcag aacgatctca
     6301 agtacctcga gctccctgaa gcaatcgacc tctcctctgt tgattatgca gaagcgtacg
     6361 caaccgtcca gacaaaggca gtgaaaggca atgggacaac gctgtacgtg ggagcaccaa
     6421 tcgtctatgg agtcaccgta cccaagatcg ccgagcaccc tgattacggg ctcctgttcg
     6481 tgaagatgct gatcggcccc gaagggcagg ccattctcac cgctgacggc cagccaccga
     6541 ttgttcctgc cggaggatac gggactgtcc cctccgagat cgccggcatg gtctccatga
     6601 aatcctgacc tcttctcttt tttaccagca gacggggatg aatgcagatt gaaaaaagcg
     6661 gataatgcat ctcaattggt ctgattcgca ttggccttaa ggaacggggc aggacatggc
     6721 agataagggg aaggtctcgt cgtgggtaag gtacaggcgg ccggactcgt gcctgctcgc
     6781 attcacgctt atcgggtcaa cgctcgtgat atttgcgttc ctggccctcg cgacgatcac
     6841 ggtcacgcag cttgcagacc tcccgttcct catccaggtc gcactcgact cccgggtgat
     6901 agattccatc ctcctcacgt tcggcgcggg tctcgcggtg gtgggggtcc tcatgatact
     6961 cgggactccg ctcgcgtatg tccttgccag gacccatttc cggttaaaca gccttattga
     7021 agccctcatt gacattcccc tcgtgctccc gcacacagtg gccgggatca tggtgtacct
     7081 gctcttcatg cagagaggcc ccctgggtgc acccttcaag gatatcggga tcgtcttcga
     7141 ggacgcgttc cccgggatcg tggtggccat gctctttgtc gccatgccct tttatatcaa
     7201 taccgtcagg gaagggttcc agaaagtccc gctccacatc gagaacgttg cccgcaccct
     7261 tggggcgagc cggttccggg cctttgtcct ggtcgtgctt cctctctcca cgcggcatat
     7321 cctgaacggg tgtgtactcg cgtgggggcg tgcgatcagc gagtttgcgg cagtcatcat
     7381 gatcgcctac tacccgatga ttgtctccac gctcatctac taccggttca cgacgggagg
     7441 gctcaaggag agcagtgcga tcgcgttcct gatgatagtc gcaagtttct cggtgttcct
     7501 ggtgctgcgg attgtgacaa gatacctggg gagatatgat gatcgcgttt gatcgcgtaa
     7561 gccttgccct cggcgagttt gccttaaagg agatctcatt tcgtgtcagg gagggggatt
     7621 acttcttcat catcggcccg tccggggcag ggaagacgat ccttcttgaa gcgatcgccg
     7681 gactctacgt ccctgacaag gggaggatcc tcctctctgg gagagacgtg agtcagatcc
     7741 ctcccgaaaa acgcggtgtc ggtctggtat accaggatta ttcgctcttt ccgcacatga
     7801 acgtagaaaa gaacatcgcg ttcggcctga agatgcaggg ggtcccggca atggagatga
     7861 agagaaccgt ggacaacctc atctccaggt tcggtatcgg gaacctccgt gcacggtcac
     7921 caatgactct ttcaggagga gagatgcagc gggtcgccct tgcgcgtgcg ctggccacca
     7981 gcccgcgtgt ccttctcctg gacgaacccc tctcggctct cgacccttca atgaaagaat
     8041 acttcatcca agaactccgg gaggttcacc aggatactgg tctcaccatc atccaggtga
     8101 gtcatgacag gcacgagatc ctctcgctcg ggacccggat ggcacttatc atggacggat
     8161 gcctcgaaca ggagggatct gtgcgggagg tctatgaatc tcctgtttcg cggagagttg
     8221 cagagttcat cgggtacgag aatgtgattg aaggagtgac aatctcgtcc ggggacgggg
     8281 aatgcctgat cgatgccgga ggcatgcagt tgcgtgcacc ttttgccgtg ccggaaggaa
     8341 ggcctgtcac cttctgcatc ggggccgacg aagtggtaat ccatccgtgc ccggtggcgc
     8401 ctgtcaatga cgaaaacgtc atccccggag tggtgacctc gctcacccag gccgggtacc
     8461 tcacccgggc aaagatcgat gcagggatta cgcttaccgc ggtgatgata cgggaaaccg
     8521 cagaagaaat ggccttgaaa ccgggttccc gcgtcagtgt ctcgatcagg aagaaagcca
     8581 tccgggtgat cgtgcaggat tgacgtgagt ctccttcata atcaggggaa cggacgcata
     8641 tccactttgc cggaaatgtc atctgcatgc cagggcatgc aaacaaattc aacccggccg
     8701 ggggcaggct attccatttc aatacgtgaa aattgggacc ggggtccggc cattcttaca
     8761 ggtggagagg cagcatatgc ccgttttgcc aggaattcga tatccttatt agcgattacc
     8821 tcatattgtg tagagcaaaa tttgcgccga tagtgtagtg gttatcacta ggcgttgcca
     8881 acgcctaaac ccgggttcga gtcccggtcg gcgcatcatc ttctctgaaa tttcactcgt
     8941 gaatcgctcc tttctccctg ttgtctcgtc atgatcggta tgagggagag gcacaaactg
     9001 aattccggct ttcgatttta tgcacagcct gaacgcgact gcctggaagg tggaagggag
     9061 atcccgaacg caccttcaaa tatggtcgga acacaaagga acagagacgg atactgtggg
     9121 accgattgga aatatctcat cgaggaggag agtgtgccga tcgaatggca cctcccggaa
     9181 tgacttgttc ccttgggtgg agtgattgcg atgcaggagg caatcgaagt caaatccctc
     9241 tctaagaagt ttgggaccac tgttgcggtt gactgcatct ccttctcggt aaaggaggga
     9301 gagatcttcg gctttctcgg tccgaacggg gcagggaaga ccaccaccac ccggatgctg
     9361 acaggggtta tcccgccgga cacaggatca gccacggtcc tctcgcatga tatcagccgt
     9421 gaacctgtgc tggcaaaaca gggattcggc gtggttcctg agacctcgaa tgcctataat
     9481 gacctctctg catggcagaa cctgatgctt gtgggggagc tgtacggcgt atcccgggac
     9541 agggcggctt ctcgtgcatc acgtctcctt ggcacgctcg ggctccttca gagaaaggac
     9601 cagaaggtcc aggcatactc aaaagggatg aaacagcgtc tcattctcgc gatggcactt
     9661 ctccacgagc cgcagctcct tttcctggac gagccgacga gcggcctgga tgtgcagagc
     9721 acacagatga tactctcgat gctgcgggag ctgaaccggc agggaaccac catcttcctc
     9781 accacacaca acatgcagga ggcgaacagt ctttgcgacc gggtggggat cattcatgcc
     9841 ggaaggatgg tcgcggtcga tgcgccggaa cgactcaaat ccgcaatcga gcgcgtacac
     9901 cggatcgagg tgagtttcaa ccgtgaagtg cccgacggcg cccttgatgc gctttacggg
     9961 gtggtcggtg tgaaccgcac cggcgacaaa tggcagatct ccgccaatga ccaggacacc
    10021 gctatcaaat cgctcgtgga gttctgccgg gatcacaacc tggcaatcct gaccctcaac
    10081 acacatgccc catcgcttga cgaggtgttt ctccagttga cacaggagag ggggccatga
    10141 acccggcagg gctccccgag cagatgagga gggcgtgggc agtggcaaag aaagacatcc
    10201 gcatctacta cgtaaagggc cccgtgctga tctttggcgt cttcatgccg gtcttcctct
    10261 ttcttgcgtt cctgatgggg ggaaggcagt tcccgctcgc gttcctggta tcagggcttg
    10321 tagggatgac cctgttcttc actgcaactg ccgtttcccc tgccatattc ccctgggagg
    10381 ggcaggccag aacccttgaa cggcttgctt catgcccagt cgcggtcgaa gcaatcgtgc
    10441 ttggtgatat ggcggcgtcc acgattttcg gcatcgcaat cacgctggtg acggttgcaa
    10501 ttggcctcat gcttggcctc actctcctga attgcttcct tatgttcggt gcaataatcc
    10561 ttgcggcctg ctgcttctcg gcgataggga tgcttcttgc agtaccgccc acgaacgtcc
    10621 cgtcaaatat catgatgctc tcttctctcc tcaagttccc gcttgtcttc atttccggca
    10681 tctttgtccc gctggagcag ctccctccct ggggaatgac atttgcagtg tgctctccgc
    10741 tcacctatct caccgatgtc atccgttact cattttcagg cagtcactat tttcccctgt
    10801 tcttcgacat cgtgatgctc ggggtcttca cattgctttt ttccgggagt gcaatgatcc
    10861 ttcacaaaaa attcctccca atgaggatgt agcggatgcg ggcatttgag gggccggcag
    10921 ggaatcgtta cacccttaac catggtgtac cctgttggtc ttccatcctg cctgtcgcgg
    10981 cattgaaaac cagtactgct cacaggggtc ctggaaaaag taagtagtgt gtatgtcacg
    11041 tccgggttcc aaataccagg agggtctcac cctgtccgtt taccaggatg agcgaattat
    11101 cacctggcac atatgcgtcc acctcctgta ttcgactgag gtagaggcgt tcctggttcc
    11161 tcacggtctc gttccggcac catgtatcgg ttgcaagcgg agtctctatc cggatcgcgg
    11221 cgctgcggat atatgccggc gcaacatagc agttacagcc tgcaaaacct gagaccgtct
    11281 catcaaggaa ccgtgcggtc acaagaaccc cctcaggaga atttacgata aacccctgat
    11341 cgtcccgcaa ggcagtcagg taccaggtct cctggaggat atgggctgaa tcttctgggg
    11401 cggtctcctt cccgaagagg agcactgccc tgccattgcc gtccaggagc gacaggaagt
    11461 ctccatccac gcggtatgta ctcgctttcc ctaaatcgag gagatacatc gactcctggg
    11521 aatgcactcc cgagggtgta ttgcacacgg catttgtccg ttgcggggca tcaatcgaga
    11581 ccttggaggg agaggcctcg taccgcgccc agtaccggtt gcatccggag cttccataca
    11641 taacgccctc gcgctcgaag cgggcagtga cgacactgcc ggggacaatg ctctcctttg
    11701 cgagcctccc gctgtcgtag gagaccagga cccaggtgat tcccgagatg ggaagcgtgc
    11761 cctcctggat gggtgtggga gtgaccggag aggagttcca tccggactcc tccggtcctg
    11821 ttcctggagg atgggtgatg catccggaca ggaggactgc gaagacgagg agtacggcag
    11881 gaatagcaca cttgctcaat ctcgtaacgc ccctgcagcg ctccttttga tatcgctgat
    11941 gcgtccggtc cggcggtgcg gttgtcatcg ctcctgactc gtcaaactct aaaaaaggag
    12001 tacatacttt cctgcgtctc tccgtgagaa gacacgggcg taccactctt cacaccctct
    12061 tctcccgcat cttcaggata tagtcgtcaa gtgagacccc ggtctccctc aggaggtccc
    12121 tgtgttcggt ctctcccgtc tccacggcat ctttcagtgc gtttcgtgca gattccgcgt
    12181 agttctgtgc gaggccgaac tggaggtcca tctcggccgc cgcagcatcg gcagtatttc
    12241 ccgcgacttt ccttatggcg gagagatatt tcaggtattc atttctccca acctcgttct
    12301 ccttctcctg gaaagattcg atcctgatca tggaggtatg ggaggtgtca gccaaggctc
    12361 tcaattcaag tgcagaggag cgcagctcgc ctgccaggta gggggcgccc ctggtcgcgg
    12421 cgccaaccgg gaaatggcgg ttcatacgtt caagcgcgga ctcgatgtct gattgtgcgg
    12481 tatcgtaaag ggaaaggaac actgcatcgc cggtttggtg tgccccggtc cccgcagcgg
    12541 taccgggagg agtggtggtg gaggtggagg ggaccatgca tcctgcagcg gctaccatga
    12601 agagcgcaca ggtgagaagg atcatctggg gtccggagtg agatctcatg aggtactctc
    12661 ttgcacatgg gccgtcatag caattttgat gctctctgaa agagacactc caaaggtgcc
    12721 tatagacgat cctgcacttt cctctttcgc gtgtggcaat gcaggaaaga tctgtattgc
    12781 ttccattgaa cgccggacag gcagggcagg gtgaaaagac atatgcaggt gcgcaggact
    12841 gttgtctata ccattctcat tctattgctc gccattgccg cggcaatctt tgcgggcatg
    12901 cattttatga tgatccctgc catcgcgcat atcgagtcaa gagaggcatc caacagtgtg
    12961 ctccggactg tggggatggt ggagaacgag cttggttctc ttgaagggac cgccatcgac
    13021 tggagccagt gggacgacac ctactttttt gcaacggatc ggaacggaga gtatgtaaag
    13081 aacaacctga tggataagac gtttacaagc ctgcaactca acctcatgct gtattatgac
    13141 ctcaacggga ccctttttta cgggaaaggc tatgattacc tcgaattcga gcgtctcgaa
    13201 attccgcagg cacttgcggt caaccctgca ggggacattc tcgacgagat tcgcaggaac
    13261 gaaagagtgg aggatgccag tatcagaggg atcatcagtc ttcctgaagg gctgctcctc
    13321 ctctcaatca acccaatatt gaagagcgat tctacggggc cggcggcagg ctacctctgc
    13381 atcgggcggt accttggtga cgcggaggtt gcaaagattg cacaactgac ctcgactgac
    13441 ctctcggtca tcagcgcagc ggatgcgggg actgcgtggg gggttatgca tgagaccctt
    13501 tccacacagg aggggtctgc tatcgtgagg atctctccgg agggaaatac catcacttcg
    13561 ttcgtcgtga ttgatgatat ccatggggtg cccgcagcag tattccgggt cgacagtccg
    13621 aggactaccc accagattgc gatggagagc ctgctcctct ccctgtacct catcgccgca
    13681 atcgggtgca tcagcgtcct tttcgtcatt tatatcctgg accacaggtt cctgaaaagg
    13741 ctctcccttc ttgaaaagaa gatgaaggag atcgcggaca ggcaggattt ttcagcccgc
    13801 acccggctct ccggcgacga tgaactctcc agcctctctg cgaagattga ccatactctt
    13861 gatgcacttg aggagcacat caacgcagaa cgcgctgcgg tggagagtgc gaggattgcg
    13921 aatgagaaac tgacactgct ctcgaagatc acgcgccatg acattttaaa ccaagttacg
    13981 gtgatcaggg gttttgcaga cctgtcccgt gatgccctgg cttccgactc acctgcggtc
    14041 ccatttatcg accggatcag ttccgcgtct gcggctatcg aagagcagct ggcattcacc
    14101 cgggagtacg agttaggcgg ggaccatgcg aagccggcgt ggttcaacat ccgtgatctc
    14161 gccaccggca ttgtaaggac catgcctctt tgtgaaatcg atgtggacat cgataccggg
    14221 gatctcgaga tatattccga tccgctcctc gagagggtga tattcaacct ggtggacaat
    14281 tcacttggtc atggagagaa ggtgaccaca atccggctca ggttctatga ggaggaaggt
    14341 tcaggcgtcc ttgtctacga ggacaacggt gtgggaatcc catatcacca gaaggaaata
    14401 atcttcgagc aggggttcgg gaggcatcgt ggcctcggct tattcctggc aaaaggggta
    14461 ctctcggtga ccggcatcac attaaaggag acgggtgtcc cgggagaggg tgcaaggttc
    14521 gagatgcggg tcccgaaggg actgttcagg ctcaaacaag gttgaaaaaa ttgttcaccc
    14581 tcatcaaagg tccttttttt ttcgttcgcc agacaggata aaaaaattca gggggaggag
    14641 ccaggccccg ggacatacca ctccggcagt acgaaatcgg cctccttcaa atggtccggc
    14701 cacactatca ctggtcggag ttcctttgtg gtctcgttcc agaccagctg ctccatgtag
    14761 agatcgaact ttgcctcgcg gaagtcgtca gtgaaggtga tcactccacc ctgcatcgcc
    14821 tctatcatct gtggcatcgt gagcgcatcg agcgcggctc tgacttttac cttctccctg
    14881 gtccctgcat tctcaaccgc ctgagccgcg atatagatcc cctcgtaggt ggatgctccc
    14941 atcatccccg ggtatgaacc ccatcgcttc tggaagttct cccggaatgc cgccatgtca
    15001 tctgcaatgt caccccgcgg tattgcatag gggctgaacc gggtctcgat gattgagtat
    15061 tctccatact tccccagtcc ttcgtagtag tcggggtcgt cattgcattc cactgccagg
    15121 aatatggtgt tgaggcccac gtccctcctg gcctgtgtga ccatcggaat ctgctcattc
    15181 aggaacgcgg caggatagac cacgtcaggc tgggctgcct ttatggtggt aagcactgtc
    15241 cggaaatctg tctcgcccat cttgaaggtc tcttctgcca caatcacgat attgagtccg
    15301 cgcctctcta tggtatcatg cactgcgttc tgcacaccct tgccgtacgg gctgtcctgg
    15361 acgagcaggg ccaggcggag cgggcggtta tcagggtact tgaaacgggc gttcactgca
    15421 tccctgattg catcgtttac aaacaaggtt gtctgctccc cgtaatcgtc ggttgtcggg
    15481 cagtaatgga acatggtcgc agtatcgata tcggttctct gcgtgatggt gggagtggaa
    15541 gccccggtaa tgatgtaggg aatgccgtgt tcagccacga ttgactggtg tgccgatacc
    15601 acagcgcttg agaagccacc caccaggaca tccaccttgt cctgggtgat cattctcgag
    15661 actgctttct ggccaccctc tcttgtcgac tcatcgtcgc cctgaataag ggtgatgggt
    15721 atctttctgt tgaggtcttt tacgaatacc cctccttttg catttatttc gtcggctgcg
    15781 agcacggcag actgccagat gtccttgccg gtggtgctgg ccggcccggt cattgaggct
    15841 actaccccta ttttcagtgt ctcctgggat acgggttgct gatccgtgca tgccgcacac
    15901 agcaccagta tcaggaggag tgcgacaatt gtgcaatact tcttcttcac gtaaattcac
    15961 cccgatggat atgcttatat tgttacatgc taacatatat catgctattt gttagcaggg
    16021 tgcacgaaat gctcagggtc aacggagtga caaaatcttt cggcggtctt accgcggtca
    16081 acagggttga tctctccctc aataagggtg agatcctcgg ccttgtaggc ccgaatgggg
    16141 caggaaagac gacactgatg aacctgatat caggcatcta ccgtcccgat tccggcggca
    16201 tcatcttcgg aggggaggat ataacccgtc tctcacttga cgctgtctgc aagaagggga
    16261 tctcaaagac cttccagcac ccccgctcgt ttcccggact gaccacaagg gaagcggtga
    16321 tgatcagtgc actctatggg aacggtcggc gcccttcggt gaaggaggct cattccgagg
    16381 cagaagagtg cctgtgcagg gttggattcc cggagcagaa gatcgacagc cccatcagca
    16441 gtctcaatac catcgagctt cggaggtcgc agcttgcgag ggcgcttgca tcccacccga
    16501 aacttcttct cctggacgag ctcacgacag ggcttacgcc gagcgagggg aaggaggcca
    16561 tgaggcttat ccaggagata cgaagcgacg gcctgacaat cctgatcatc gagcacgtga
    16621 tgcggatcat catgggagtc tctgaccgta tcgtcgtcct cgatcacggc gagaagattg
    16681 ccgaggggac accagaggag atcgcctgca acgagcaggt catagattca tatctcggtg
    16741 agcgctatca tttctgacgg gagtaggatc tcgtgctcac catagagaac ctgaatgtat
    16801 cgtattccca cctccaggtg ttgtgggacg tctcgttgaa agtctccagg ggagagattg
    16861 ttacgctcat cggatcgaac ggtgcaggaa agagtaccct ggtctccacc attttcggtc
    16921 tcaattcctc tccgacgggg aggattgaac tgaaaggaga gaacatactc ggacttccca
    16981 cctacgagat cgtccggcgc cgcctagccc tcgtccctga aaaaagggag ctcttcccga
    17041 gaatgaccgt gaaggagaac ctggaactcg gcgcatatgt ccgggagaat ggatctgcgc
    17101 ttgaccatgt ccttgagctc tttcctgtac ttaaagacag gcaggcgcag aatgccggga
    17161 ccctgagcgg aggggagcag cagatgcttg caatcgggag gtgtctgatg gcggacccgg
    17221 agatgatcgt cctcgatgag ccgtcacttg gcctctcccc gaaactcgtg cacatggtgc
    17281 ttggaaccgt gcagcgcctc aacgacgaag gcctgaccat tctcctcgtg gagcagaacg
    17341 tgcgcagggc gcttgagatc tctcaccggg cgtacgtcct cgaaagcgga aggatcacga
    17401 gaaccggacc cgctgacgat cttctccacg atgaccagat aagggaagcg tatctcggga
    17461 tgtaaatcca taccccgaag ctctttttcg tgtcatatgc aagagggatg atgccatccc
    17521 cctaatgagt cttggtggtc cagtgcaggt gtaaatgcga ccccttgccc caaaaagcac
    17581 ttttttttgg ttcctggtcg gcagcggtcg cccatatcag gaatgggagg gccagggatc
    17641 gtggcgtttg gacccctgga atagccagga ccggcattaa gtgagattgg tgtgacccaa
    17701 tcaggtcttt ttctgcctga tcctcttctg tatttcctcc gggagcgaga caagtcccct
    17761 tgggaggaat atgatgataa gcacgaggag gacgccaatg atgatgaaac gggcatacgt
    17821 gaggccaacc gcattgaggc cgtcccagag cagtgtcacc acgattgtgc cgatgaccgg
    17881 tcccgcgaga gttccaagcc ccccggtgat tgaatagatg atgggccaga acgagaggtc
    17941 gattccgaat atctcagggg tgatataccc gaagtgaaat atttcgaggc cacctgcaat
    18001 ccctgtgacg aatgcactga cgccgaacgc gaagagtttg aaggggaccg ggttgatccc
    18061 ggcggccctt gcctcgactt cattctcccg tatcgcagca agggcaagcc ctatcctcga
    18121 ctggagcagg taccggaaga tcaggatgac cccgattgcg atgaagagga tcaggtaata
    18181 ctcgatcatc aggtaccctg gaatagtggt tggcacgagt tttggaaccg ggaacccgtc
    18241 ccagccccct gtgattgggg cgagctggct cacgatgagc gtctggatga tcaccgcaaa
    18301 accgaatgtc accattgcga ggaaccattc ccgcaggcgg acgcacgtga ggccgatgag
    18361 gatcccgatg aaggccgcga agcatcctcc aaagagcagg gtgacgagtg gcgggaggcc
    18421 gaaccggata gcgacaaggg atgcagcgta ccctccgagg ccgaagaagg ctgcatgccc
    18481 gagcgacccc tggccagaga aggtgaggaa gttccaggca gacgcgtaga tgatgaagac
    18541 cagcatcagg atgaggatag tcagataata gaggttcgat ccgatgaaga tggggatgag
    18601 aaaagctatc accaggaggg ccgcggcgag caggtggcgg tagtcccgtg gatcgaatat
    18661 tttcattctt cagcctcccc aaagagcccg gtaggccgga tgacgaggac gacaatcagg
    18721 agaatgaagg ataccgcatc cttccaggag ccgccgaggg tgaacgcggt gaagttctcc
    18781 accatcccga ttgcaaggcc accgatgatt gcgcccggga tactcccgag cccgccgagg
    18841 atgatgatag caaagccctt gatggccggg atcgagccca tgtaggggtt gaagacctgc
    18901 ccgctcaccg cccagagggt gcctgccgca ccggcaaggc cggcaccaat cccgaaggtg
    18961 agcatgtcca tcctctccac gttgaccccc atcagcatgg cgcccttgcg gttctggctc
    19021 gcggccctga ttgcccgccc gagcggggtc ttctggagga agaggtagag cgagacaagg
    19081 atcagcgcgg tgaccgtgat aatgatcaga taatcaaggg gcaattggag gggacccagg
    19141 ctgaccagga tatctgcaaa cgggctcttc attgacctcc attcgtcggt ccagatcatc
    19201 gccaccaggt tctggaatat gtaaatcagc ccaaggctta tgaatatctc gttcagcttc
    19261 cccgtgccga gaatcggccg gaagcacaac cgctccacga tgactcccac gatgatgagt
    19321 atgggaatgg aaccgatgag gaggacatat gggttgaacc cgctgataac aaagaggaag
    19381 aacgtgatat acgttccaag catcaggaac tcgccatggg caaaattcac gatcttcatc
    19441 acaccgaaga taaggttgag gccggtggca agcaggatgt agatgcatcc cgaatagagg
    19501 ccccagaagg caacctggct gattatcccg atggtcccgt ccatacagta atccttctcc
//
LOCUS       DAOQYR010000037        19502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_3165_length_19502_cov_8.547385, whole genome shotgun sequence.
ACCESSION   DAOQYR010000037 DAOQYR010000000
VERSION     DAOQYR010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 19502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19502
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3165_length_19502_cov_8.547385"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="PKM87_06010"
     CDS             <1..1395
                     /locus_tag="PKM87_06010"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNQ29656.1"
                     /translation="CIISGSPKGEESITLQYVRFIEQAFPEHTFPVVHAGRDIKKMEQ
                     QREAWDEALAKVYDSDGVVFATPVYFMLVPGQLKRFIEMAFERDARSAFSQKYSAALT
                     TSIHYFDHTAHSYLHGISEDFGMHHAGSYSAHMEDLKSEVEQKRLLLFFSDFLDAISE
                     KRAITREFLPLPKVSPTYYPSTQSTVVDTNGRRVVILHDAKPGSDQEAMVRRMAALFG
                     GKATVADIGDSLMKGGCLGCCRCAFDNTCVYQDGFRDFWERYVVPADIMIMCGTIQDR
                     YLSATWKQWNDRSFFNGHVPALKGKQVGYLVEGPLAYLPNLREVLAARAVIGGANLVG
                     IVTSEQGNPGEIDAALAALAERSVRFADKGYFAPVMFSAIAGRKLFRDEIWAHMRAIF
                     QADDRYYRSHGYYDFPTKNYLQRIGTRAFSLFLAIPGVRKKVEKDMGSYMVRPIRETV
                     QKSRVIKEWRARQE"
     gene            complement(1562..2479)
                     /locus_tag="PKM87_06015"
     CDS             complement(1562..2479)
                     /locus_tag="PKM87_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ29657.1"
                     /translation="MHIRLADHILLLHMRITQSVHALCIPFQISAAPGIVLDRSVYCS
                     IVFGETITLIDTGVAGSESLIFDTIRSAGRHPSEIEWVILTHSHPDHIGAAASIQDAT
                     GCSIAAHPAERQWIEDVNLQNHERPVPGFASLVSGSVQVDRELIDGNIIFLDKSRELS
                     LEILHTPGHTPGSISLFMDCEGVLFSGDAVPVAGELPIYEDAPASARSIRRLQGIAGI
                     EVLVSAWDAPRRGEEAYDRLEQATGYLQRIHETVCAHRGTDSRDLMALSRKIATSLGI
                     PPVAFNPLLARTFAAHLRVCRYQNILESF"
     gene            2564..4027
                     /gene="cls"
                     /locus_tag="PKM87_06020"
     CDS             2564..4027
                     /gene="cls"
                     /locus_tag="PKM87_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HNQ29658.1"
                     /translation="MIEWLMIWDTVLLFIIVLNIGFVISVVFFERKNPSATLAWVMAL
                     LLLPVAGFILYLFLGQTYRKDRMFCIKKEEDQKLREIIESEKEELKKEGAMTRADHTS
                     TKLRRMVCMLLENNDAILTLNNHVNVFADGNEKFDALIEDIEGATLFIHLEYYIWKND
                     GIGNRMKKALTERARAGFEVRLLCDGLGCAGLPGDFFDEFKAVGGKVAFFFPSFLRIL
                     NLRYNYRNHRKIAIIDGKTCYIGGFNIGDDYLGLYEKWGYWRDTAVRIYGNAALTAQI
                     RFFLDWNYAAPDEHLDYDDRYFPDTPMIEGIPIQIVSGGPDTEFNPVKESYLKMISIA
                     TETVYLQTPYFIPDESVLDALRIAALSGTDVRIMMPDRPDHPFVYWAGMSYVKQLLDS
                     GVKAYTYDNGFLHAKTIVIDEIAASVGSANWDVRSFRLNFETNAVMYHPEVAKTLKER
                     FLCDLEVCTPITSGRLDSLPWSRRILMSIGRLFSPML"
     gene            complement(4174..5034)
                     /locus_tag="PKM87_06025"
     CDS             complement(4174..5034)
                     /locus_tag="PKM87_06025"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNQ29659.1"
                     /translation="MIPRIRFGKPDIAVMKREGYAAVDMHVHSNHSDAVPSVRKILNY
                     ARRRGIGVAITDHNEISGVREINGYRPDALVIPGIELDCAEGPHILLYFYSCNDLADF
                     FNRHIRERRVGARYTKSFLATDEILTLAEGYPCLKVAAHPFGYFFLDRGILKCDARGI
                     LPGIVERLDGIEVICGGMTMRVNRMAIDYANSRHIPVTGGSDAHIQSEVGRVVTGVRA
                     ESVGDFLDGIRRCESVVAGSSSGIASRMVTGSVIGYHYLPYSVKVLHSGMGSHAGRIM
                     HSMRRIFRPR"
     gene            complement(5116..5304)
                     /locus_tag="PKM87_06030"
     CDS             complement(5116..5304)
                     /locus_tag="PKM87_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29660.1"
                     /translation="MNTSHMMILIFAVFLLVPLGFFFLVISLGNFMYGDSIAGLVFLV
                     IFMACSGAVYFLLKKYRE"
     gene            complement(5393..7744)
                     /locus_tag="PKM87_06035"
     CDS             complement(5393..7744)
                     /locus_tag="PKM87_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNQ29661.1"
                     /translation="MMFRADPWHSGINDDGGIMPLDTLIWMNTTGDRVYSSPAVVDGV
                     VYVGSDDHTVYAFEAESGTEVWTFETGGRILTSPAVADGVVYVGSYDKSLYALDKDTG
                     SMLWNFPMEGWVTADPVVTNGVLYSGSLDGNLYAINATTGGELWNASFGYYPSIDSSP
                     CVAGGNLYVAGGQRTLYALDAIDGSEVWNFTSGDFISSSASVADGIVYFACYDRKVYA
                     LNATTGAQIWTFETGGGGEVHSSPAVAYGVVYIGSCDSNVYALDALTGEKIWNFTTGN
                     VVYSSPTYANGIVYVGSWDHDMYALDSLTGEKIWNYHTQAPIFSSPAVANGIVYFGSL
                     DDNIYAVGHPLADIPVANFTANETFGIIPMAVQFTDLSTGGGPRSYQWDFGDGTPNST
                     EQNPVHTYTVMGTYDVTLTASNAAGSDTLKKEWYISAFEPMPPVGGDKAYFLVHSNVE
                     GAEVYFNGDWLMGTIENGTLLVQTCTTCTPVRSYTVKKCGYFPFTQQNNRFPGKDETV
                     HLHANLTAPKEPLIADFTANVTEASAPPLTVQFESHCVGIAETWNWSFGDGTFSQERD
                     PVHTYTNFGNYAVSLHVTNSACQENTMEKVDFIQIMEKPPFYADFTVTPVWGYIPLTV
                     RCTDQSHGNPTTIIYDFGDGSRGMGANVAHTYRFPGTYTITETISKYDTATRSVIKSV
                     AVKPGAVEVARPFILPLSAGFSASPVTGTVPLTVIFTDESFGNPTFYIYDFGDGFKSM
                     DPNPAHTYRFPGRYTVSQKVLRVGHGTMQSDVEVKQDLILVSA"
     gene            8081..8653
                     /locus_tag="PKM87_06040"
     CDS             8081..8653
                     /locus_tag="PKM87_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29662.1"
                     /translation="MKTHTPTTPLKALISGIIGIVIFLVGLVVLRFIAHHTSWPLFDG
                     FVDLLFAHAALIIFFSILFTIGEIFAAFSFPFNLPFPVFNAVASVLLVSFLISLLVYV
                     NDFYAIGIGHALGVVRLFLLPLTLIIVLVAGYLSIFVKMKGPEVTPSSPSGGSTEPGR
                     SCPSWETIGEEFRQMIADLIRKIRNEINKD"
     gene            complement(8689..9204)
                     /locus_tag="PKM87_06045"
     CDS             complement(8689..9204)
                     /locus_tag="PKM87_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HNQ29663.1"
                     /translation="MPARILVAYASVHGSTAGVAEAVGKALQEEGYTVDTAVMKTVSS
                     PGDYTAVVIGAPVYMGKMVGDVKKFVGKHRDALVQVPVAAFCVGVAPLGKDPGDVDTI
                     MNIFRTALAPLEPVDVTMFAGKIDMEKLPFMHKWIWKKMDGPVGDFRDWEAIRVWARD
                     LPRKLGISAGA"
     gene            9421..10080
                     /locus_tag="PKM87_06050"
     CDS             9421..10080
                     /locus_tag="PKM87_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNQ29664.1"
                     /translation="MATFVLVQGGNLSTDTWNKLAGRSDYPPGGQLGGAYWNGTVEAL
                     AAHGHQAFAPTLEDEHKTGLAGHIRQIYSLFTGNSLKDIILVGHSYGGMIITGVAAAM
                     PGRIRHLVYLDAALPDSGQSLFDLFALAGSDPLSFIGLEPAKAYTVKIEFDPRHLESI
                     PKTYILCTKSEFRIVTGLAKRKIDADRKGWTYLELPSSHVPMADMPGELLNILLDIAE
                     R"
     gene            complement(10334..11641)
                     /locus_tag="PKM87_06055"
     CDS             complement(10334..11641)
                     /locus_tag="PKM87_06055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29665.1"
                     /translation="MRMVAVLSGLVILLLICALALLAFQSPAPTLHPGSLDCTFNSPQ
                     GYVMSTHAENEKDRGVEVAIQDDGHIVALGYCNNTQNEDLMIARYTSYGALDSQFGSG
                     GFAYYDGGGNDRGLGLVLQGDGKMVATGFTQNGSHRDLLLLRYDLNGTVDPTFGNEGV
                     VMFSAPGSSTDIGFGVVWESDGGIFVAGETANATHQDALVIHYTPEGVYDTRFAGTGL
                     FTYGGTNMDRLFACALQPDGKILVTGSSAANEKDDLLLLRMNHDGTLDGTFGVGGVVL
                     YSGEGDNFDYGNWVLVQPDGKILVSGATSVDNAFDLLLMRYNPDGTPDPAFGIGGVVH
                     HGDAGGRDEYGYAHAVQQDGKIIVAGYAENATYDDVFVVRFYPDGTLDTTFGNGGQVL
                     WNGPGDHKDYGQGIALQRDGKIVVTGFTHHDASEDLLVMRLWP"
     gene            11924..13207
                     /locus_tag="PKM87_06060"
     CDS             11924..13207
                     /locus_tag="PKM87_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ29666.1"
                     /translation="MKYPTLAIAILLTFLMLAAGCMNTAPPQTTGPGSGVSSPVVIDA
                     LVTLTGPYSSQGAIAKAALLTAEEDINRYSAAIGSERRVSVVFHDTASDPATALTQAK
                     EVQKSGRRIILGHMTSAEIAAIKTFVDANGMLVLDAGSTSPSLAFPDDSIYRLISDDS
                     AQGRAMAMWLEEQQIQAIVPLWRGDVWGDNLLNATRSAFLERGGVAIDGIRFGPDTTD
                     FSFHVASLDLLAGDAIQAHGADHVGIYLIGFEESVPILEEISVKENLTRVRWFGCDGN
                     AGIPSLAGVSPAASAAARVNLTSVVWGIAHGDPSSEPQTRIRERLGYSPSSGPIALYD
                     ALWVVHDLLNEIPSNADQTRLKLALERHLNTYQGETRSLATNEGGDRALASYDVFQVM
                     ERDGIPQWKYIAHIIIWEDRSEHIGTDTCPYFRQE"
     gene            13332..13883
                     /locus_tag="PKM87_06065"
     CDS             13332..13883
                     /locus_tag="PKM87_06065"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNQ29667.1"
                     /translation="MAFRLTTERLILEDLNEDDLDNIRRIACNKEVMRYVLVWLENED
                     QIVGFLQRGIEEAKRPDRTAYILAARVAENGEFAGLTFLEIDPQIRCTGEVGIVLLPE
                     YQAFGYGKEILKTYMEFGFDSLSLHRVFGKCDEMNHASARLMEQCGMTFEGTIREHVW
                     LRDHWRSTRYYGILAGEYAALNP"
     gene            complement(14061..14756)
                     /locus_tag="PKM87_06070"
     CDS             complement(14061..14756)
                     /locus_tag="PKM87_06070"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNQ29668.1"
                     /translation="MKRVSILQIGDMQNGFIQKEGNLYVPGAENLIDPANDFLRQVSE
                     DVFDLTLIVLDTHFKEEYYETQESRQFPIHCEYGSRDWELAIDLKGAPNIRYMMKNNF
                     SMWTNLRKRALQCTDPVKRRVYKNLFHILDDPHTPSECIPRDEFLKAFFSGSIPAEVE
                     VTLIGVASDYCNRYSMEGWLSRGARVTILQDLTKGIEKETPLVLAEDRYRCYIPESLR
                     AVESTVYLKEMAD"
     gene            15013..15696
                     /locus_tag="PKM87_06075"
     CDS             15013..15696
                     /locus_tag="PKM87_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNQ29669.1"
                     /translation="MGEIIDDPLLRERTRVFSGRHDAGRRLGVFLLSLPPTSEPLVLA
                     IPAGGVPVGKEIAKTLGAPFSLAIVRKVRIPGTTESGFGAVAWDGTVLINERLRDALF
                     LSSSDVERAVSETAANVKERITRFMAGTPMPSVAGKTVILTDDGLASGFTMLAAIRSL
                     RVRGPARVVVAVPTASRSSAELVGREADQLVCLNIRSGRSFAVADAYEEWYDLDDSEV
                     LAELTSIPR"
     gene            complement(15828..16664)
                     /locus_tag="PKM87_06080"
     CDS             complement(15828..16664)
                     /locus_tag="PKM87_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29670.1"
                     /translation="MSTIPRSTNHSLRAILPGILILAFLLSAAGCSNIPGGGAASHNF
                     WANITGQSGDACSFDRLTGQTDVHLTQGDSCYFTTHTPIEYLDDLRMHPRSHVLVIDV
                     PRDWITPADVEHLMQEIDSTEPAAPVVSPLSSYRPFNQTSTVGNEALYLIEGYRAGKY
                     PPALCSLHYFHPDKDEVRSWWEAYGRWNYPEEREAIRLIKEAYPDLKGYPADTFPVKS
                     IRTERAPEGWYVAFIQEGSGVPIISARCYCVGNDRNITLTGTANQSVMVMVPEFSPKT
                     CK"
     gene            16859..17281
                     /locus_tag="PKM87_06085"
     CDS             16859..17281
                     /locus_tag="PKM87_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNQ29671.1"
                     /translation="MTDIAVFVDSRGGNTKKVADTIAAELGVAAGDPAAMLPADAKVV
                     FLGSGVYGGKPGKAMTAFIASGDFSGLKVALFATSGGAAGADKMLGTMADALKEKGAT
                     VIGNFHCRGKYLFTNRGHPGPEDLENARKFAREMRANS"
     gene            17563..18789
                     /locus_tag="PKM87_06090"
     CDS             17563..18789
                     /locus_tag="PKM87_06090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNQ29672.1"
                     /translation="MIRSGYIFFTFIVALAILSAGCTQPSVEHQSPAASSAVNITVPV
                     PPIPVRSSGGMNLAYELELTPDNGLKFVPEDVEVIDPATGKVLYIMEKELFASLYHPA
                     ADPLPSGTELQNGTGKVPVPRISIWFVVDPNAVPDRLVHRVTLNMSGSGQPPFTQTGG
                     EIAVRKDLSPVVVGSPMRGPGWLALETTAPDTHHFSAQITMDGVTRVPQKYAQDWIFV
                     DPLTGKAVEGDSALARNYYGFGKEILSVANGTVVDMLDGLPDHEMIYSPQGVTIATAA
                     GNYVIVDIGDGKYACYAHMVNGSVRVAPGDRVTDGQVLGLMGNSGNSDLPHLHFQVVE
                     GKASFLGAEGYPHVYRAFNVTGEVDQARGIEMFSDPEFTTEKLWSEFDILVSSYPNPV
                     YQQESLPENWVILDLP"
     gene            complement(18999..>19502)
                     /locus_tag="PKM87_06095"
     CDS             complement(18999..>19502)
                     /locus_tag="PKM87_06095"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNQ29673.1"
                     /translation="RIVVRVNALWHRDPAKAIFDANNIITEDSKSGMFVTLFFGALSE
                     SDRTLKYVNAGHNPPVVVRSRDGTLEELLPTGMAIGADAHQEYASRTIPIGTGDVVVI
                     YTDGVTDAMNAQEKFFGEDRLYTIIRENARLPAQEILDRILSEVREFSKDMPQFDDIT
                     VLVVKGN"
BASE COUNT         4408 a         5434 c         5268 g         4392 t
ORIGIN      
        1 tgcatcatct ccgggagccc caagggtgag gagagtatca ccctgcagta tgtccggttc
       61 atagagcagg catttccaga acataccttc ccggtcgtgc atgcgggacg ggacatcaag
      121 aagatggagc agcaacggga ggcatgggac gaggcgctcg cgaaagtcta tgattccgac
      181 ggcgtggtct ttgcaacacc ggtctatttc atgctcgtcc cgggccagct gaaacggttc
      241 atcgagatgg catttgaacg ggatgcccga tcggcatttt cgcagaaata ttctgcggca
      301 ctcaccacct ctatccatta cttcgatcac accgcccatt cctacctgca tgggatctcc
      361 gaagacttcg gcatgcacca cgccgggtcc tactcggccc acatggaaga cctgaaatcc
      421 gaagtcgaac agaagcgtct gttgctcttt ttctccgact ttcttgatgc aatttcagaa
      481 aagagggcca tcacgcgcga atttctcccg ctaccaaagg tctcacccac ctattatccc
      541 tccacccaat cgactgtcgt ggataccaat gggagacggg tggtaatcct ccatgatgca
      601 aaacctggtt ccgaccagga ggccatggtc cgccggatgg ctgcactatt tggcgggaag
      661 gccacggttg ccgatatcgg ggattccctg atgaaggggg gctgccttgg ctgctgccgg
      721 tgtgcgttcg acaatacatg cgtataccag gatgggttcc gcgatttctg ggagagatac
      781 gtggttccgg ccgatatcat gatcatgtgc gggaccattc aggatcgata cctgtcagca
      841 acgtggaagc agtggaacga ccggagtttc ttcaacgggc acgttcctgc actcaaggga
      901 aagcaggtcg gatacctggt ggaagggccg ctcgcatacc tcccgaatct gagagaagtg
      961 cttgcagccc gggcagtgat cggcggggcg aaccttgtcg ggatagtgac cagcgaacag
     1021 ggaaacccgg gcgaaatcga cgcagccctc gccgcacttg ctgagcgctc ggtcaggttc
     1081 gcagataagg gctactttgc cccggtcatg ttctcagcca tcgcaggaag gaagctcttt
     1141 cgggacgaga tatgggcaca tatgcgggca atatttcagg cagacgaccg gtactaccgg
     1201 tctcatggct actatgactt ccctacaaaa aactacctgc agcggattgg gacccgggca
     1261 ttctcactct tcctcgccat cccgggtgtc aggaagaagg ttgagaagga catgggctcc
     1321 tacatggtcc gccctatcag agagacggtt caaaagagca gggtcataaa agagtggaga
     1381 gccaggcagg aataattggt atcactcgtg ccatgtgagg aatgcgtttg catttatttc
     1441 gcgagaccca ctcacaaaag agtcaaatgc catcaccact gatcatctct tcttctgttc
     1501 tgatcagtca actggaaaaa attaggactc tcgctcagat ctgcaaaagg gactcttctt
     1561 tttagaagct ttcaagaatg ttctggtatc tgcacacacg aaggtgtgcg gcaaaggtcc
     1621 ttgcgagcag ggggttgaat gcaacagggg gaatgccaag ggaagtggca attttcctgg
     1681 agagtgccat gaggtctctg gaatctgttc cccgatgcgc acatacggtt tcgtggatcc
     1741 tctggagata tcctgtcgcc tgttccagcc gatcataagc ctcttctcct ctcctcggtg
     1801 catcccatgc cgatacaagg acctcaatcc cggcgattcc ctggagccgc cggatggacc
     1861 gggcggaggc gggtgcgtcc tcgtaaatcg gcagttctcc tgcgacaggg acggcatcgc
     1921 ccgagaagag cactccttcg caatccataa agagggagat ggatccaggc gtgtggcccg
     1981 gcgtatggag gatctcaagt gaaagttctc tgcttttgtc gaggaatatg atatttccat
     2041 cgattaattc gcgatccacc tgcaccgatc cgctgacgag ggaagcaaaa cccggtaccg
     2101 gccgctcatg gttctggagg ttcacatcct ctatccactg ccgttcagcg ggatgggccg
     2161 caatgctgca gccggttgca tcctggatag atgcggcagc gccaatatga tcggggtggg
     2221 aatgggtcag gatgacccat tcaatctcgg acgggtggcg accagccgac ctgatggtgt
     2281 caaagatcag gctctcgctg cccgcaactc cggtgtcgat gagagtaatg gtctccccga
     2341 aaacgatgga acaatacacg gaccggtcga gaacaatgcc cggggctgcc gatatctgga
     2401 aggggatgca gagtgcatgg actgattgag tgatcctcat gtgcaagagg agtatgtggt
     2461 cagcaaggcg tatatgcatg agcccatcat tcaaatctct gccctttaat tactcccgga
     2521 cgcaatgctg gtatacgcat atcatgttat tgaggctgaa cgaatgatcg aatggctcat
     2581 gatatgggac acggtgttgc ttttcatcat cgttttaaat atcggctttg ttatcagtgt
     2641 cgtgttcttt gagaggaaga acccgagtgc aacgctcgcc tgggtcatgg cactccttct
     2701 gctgcccgtc gccggtttta tcctgtatct ctttctcggg cagacctata ggaaagacag
     2761 gatgttctgc atcaagaaag aggaagatca gaaactccgg gagattattg aatccgagaa
     2821 ggaggagttg aagaaagagg gtgcgatgac ccgggcagac catacctcga cgaaattgcg
     2881 gaggatggtc tgcatgctcc tcgagaacaa cgatgcaatc ctcacgctca ataatcatgt
     2941 caatgtcttt gctgatggaa atgagaagtt tgacgctctc attgaggata ttgagggtgc
     3001 gactctcttt attcacctcg agtattatat ctggaagaac gacgggattg gaaaccggat
     3061 gaaaaaggcg ctcaccgagc gggcccgtgc aggcttcgag gtccggctcc tctgcgatgg
     3121 gctcggatgc gccggcctgc caggtgattt cttcgatgaa ttcaaggctg ttggtggaaa
     3181 agtcgcgttc ttcttcccct catttttgcg gatcctgaac cttcggtaca attaccgtaa
     3241 tcaccggaag atcgccataa tagacgggaa gacctgctat atcggcgggt tcaacatagg
     3301 ggacgattac ctgggcctgt acgagaaatg gggatactgg agggacaccg cggtcaggat
     3361 ctacgggaac gcggcactca ccgcccagat ccggttcttc ctcgactgga attacgccgc
     3421 acccgatgaa cacctggatt acgatgacag atatttcccg gacacgccca tgatcgaggg
     3481 gattcctatc cagatagtat caggaggacc ggacaccgag ttcaacccgg tcaaggagtc
     3541 ctacctcaag atgatctcaa tcgcaacaga gactgtgtat ctccagaccc cgtacttcat
     3601 tcccgatgag agcgtgctcg atgccctccg catcgcggca ctctccggga cagatgtcag
     3661 gatcatgatg ccggacaggc ccgatcaccc cttcgtatac tgggcgggga tgtcgtacgt
     3721 aaagcaactc ctcgactccg gtgtgaaggc ttacacctac gacaacggat tcctccatgc
     3781 caagacgatt gtcattgatg agatcgcggc atctgtcggg agtgccaact gggacgtgcg
     3841 gagcttccgg ctcaacttcg agacaaacgc cgtgatgtac catccggagg tcgcaaagac
     3901 gctgaaagag cggttcctgt gcgacctcga ggtgtgcacc ccaatcacca gtggaaggct
     3961 tgattcactc ccctggagca gacgtatact gatgtccatc ggccgcctct tctcgccgat
     4021 gctctgaaca ggatattgag acacttgcaa catgcaaaaa cacgtcttcg atagggaagc
     4081 aattgcccgg aacctcctgg atatctacga cgacttgaaa gaaaaggtca gtcccgcgac
     4141 atcttcctgg acggaacggg agatacgatg gccttacctc gggcggaaga tgcgcctcat
     4201 ggagtgcatg atccggcctg catgcgaacc cattccggag tggagaactt tcaccgaata
     4261 cggcaggtaa tgatagccga tgacactccc tgtcaccatc cttgaagcga tccccgagga
     4321 tgaccctgcg accacgctct cgcatctccg tatcccgtca agaaaatccc cgacagattc
     4381 ggcgcgtact cccgtgacca cccttccgac ctcactctgg atatgggcat cggaccctcc
     4441 tgtaacagga atatgccggg aattcgcata atcaatcgcc atcctgttca ccctcatcgt
     4501 cattcccccg cagattacct cgatcccgtc aagacgttcg acgattcccg gaaggattcc
     4561 ccttgcatcg cacttcagta tcccgcggtc caggaagaaa tacccaaacg ggtgggctgc
     4621 aaccttcagg catggatatc cctccgcaag ggtgaggatc tcgtctgtgg ccaggaacga
     4681 cttcgtgtac ctggcaccga cacgcctctc cctgatgtgg cggttgaaga aatctgcaag
     4741 gtcgttgcag gaataaaagt agaggaggat gtgaggtcct tcggcacagt caagttcaat
     4801 gccgggaata accagggcat ctggccggta tccgttgatc tcccgtactc cactgatctc
     4861 gttgtggtcc gtgatcgcaa ctcctattcc cctcctacgg gcataattga gaatttttcg
     4921 aacggatggc acagcatctg agtggttgct gtgcacatgc atgtccaccg ccgcgtaccc
     4981 ttctctcttc atcactgcaa tatccggttt cccgaaccgg attctgggga tcatgtcagc
     5041 ggccatgcac ttttccgatt catatgaagg gtatattttt tcacaatcaa gactggtttt
     5101 ttggtatgct cgaggttact cacggtattt tttcaggagg aagtacacag ccccggaaca
     5161 tgccatgaat atcaccagga agacgagtcc cgcgatcgaa tccccgtaca tgaaattccc
     5221 cagggagata accaggaaga aaaatccgag cggtacaagg agaaagactg cgaagatcag
     5281 tatcatcata tgggacgtgt tcatgtcagg cacggattcc tcctttatgt cgtaattgtc
     5341 atctcacctg catgtgcgcg gaaactccgg gtaaaaagga aaagcgcata tctcatgcgc
     5401 tcacgaggat caggtcctgt ttcacttcga catcactctg catcgtaccg tgcccgaccc
     5461 tcaacacctt ctggctcacc gtgtagcgac cagggaaccg gtaggtatgg gccgggttcg
     5521 ggtccatcga cttaaagccg tccccgaagt catagatgta gaacgtcggg ttcccgaatg
     5581 actcgtccgt gaatatgact gtcagcggaa ctgtcccggt aacaggagag gcggagaacc
     5641 ctgcagagag cggaaggatg aagggtctgg ctacttcaac cgcacccggc ttgactgcca
     5701 cgctttttat tacggaccgt gttgcggtat catacttcga gattgtctct gtgatcgtat
     5761 aggtccctgg gaaccggtag gtgtgggcta cgtttgcgcc catccccctg gatccgtccc
     5821 caaagtcata gataattgtg gtgggattcc catgcgattg gtcagtgcag cgcaccgtca
     5881 atggtatgta cccccagaca ggtgtaacag tgaaatctgc ataaaaagga ggcttctcca
     5941 ttatctggat gaaatccacc ttctccatcg tgttctcctg gcaggcgctg ttcgttacat
     6001 gaaggctgac cgcgtagttt ccaaaattgg tataggtatg gacaggatcc cgttcttgtg
     6061 agaaggtccc gtcaccgaaa ctccagttcc aggtctccgc gataccgaca caatggctct
     6121 cgaattggac agtcagcggc ggggcggatg cttcggtgac gttcgcagtg aaatcggcga
     6181 taaggggttc ttttggcgcg gtgaggtttg catgcaggtg aactgtctcg tccttgcccg
     6241 ggaaccggtt gttctgctgc gtgaagggga agtacccgca ctttttcacg gtatacgacc
     6301 ggaccggcgt gcaggtggtg caggtctgca cgaggagagt gccgttttcg atagtgccca
     6361 tcaaccagtc gccgttgaaa tagacttcag ctccttccac gttcgaatgg accaggaagt
     6421 acgccttatc gccgccgacg gggggcatcg gctcaaaggc tgagatgtac cattcttttt
     6481 taagggtgtc cgaaccagca gcgttactcg cggtaagcgt cacgtcatat gtccccatca
     6541 ctgtataggt gtggacaggg ttctgctcag tcgagttcgg cgtcccgtcg ccgaaatccc
     6601 attggtagga gcgcggcccg ccccctgtcg agagatcggt gaactggaca gccatcggga
     6661 tgatcccgaa tgtctcgttc gccgtaaagt tcgcgacagg aatatctgcg agcgggtgcc
     6721 cgaccgcata tatgttgtca tcaaggctcc cgaagtagac aatgccgttc gccaccgcag
     6781 ggctcgagaa aataggtgcc tgggtgtggt agttccagat tttttcgcca gtcagggaat
     6841 cgagcgcata catatcatgg tcccagcttc ccacgtagac gatgccgttt gcataggtcg
     6901 ggcttgagta cacgacgttc ccggtggtga agttccagat cttttcaccg gtaagggcgt
     6961 caagtgcata tacattggaa tcgcaactcc cgatatagac aaccccgtag gcgacggcgg
     7021 gagatgagtg cacctcacca ccaccaccgg tttcaaaggt ccagatttgt gcaccggtcg
     7081 ttgcattgag tgcatacacc ttcctgtcgt agcatgcaaa gtagacgata ccgtctgcca
     7141 ccgaggcact cgaggagatg aaatcaccgg atgtgaaatt ccagacctcc gacccgtcaa
     7201 tggcatcaag tgcatacaac gtcctctgac cgcctgccac atacaggttt cctcctgcga
     7261 cacaaggact ggaatcaatg gatggataat aaccaaatga tgcattccag agctctcctc
     7321 ccgtagttgc gttgatggca tagagattgc catcaaggct cccgctatag aggacgccat
     7381 tcgtcactac gggatctgca gtgacccatc cctccattgg aaaattccag agcattgaac
     7441 ctgtgtcctt atcaagggcg tagagggatt tgtcgtaact tccaacgtag acgaccccgt
     7501 cagcaactgc cgggcttgtg aggattcgcc ctccggtctc gaaggtccaa acctccgtgc
     7561 cgctctctgc ctcaaatgca tacacggtgt ggtcgtcact cccgacatat accacaccgt
     7621 cgactaccgc aggactcgaa tataccctgt cgccggtggt attcatccat atgagggtgt
     7681 cgagcggcat gatacccccg tcgtcgttga tccccgaatg ccaggggtcc gcccgaaaca
     7741 tcatgggcgt gtgatccgcg gccacgcatg gagtgcctgc cgcccatatg aaaagaagaa
     7801 acaggaaaaa aggccctgcc acgccacgca gagctatact gccacctttc acacaagaca
     7861 tgatcggtga tattgcactg attgctaata acctcgcagg gtcatgatgt gggactatcc
     7921 ccagggtccc gcagtgtcca ttgtcgtatc tggtttatca ggctgattgc tccgccagga
     7981 tgggcggaat ctggagatct cgaatactgc accatccctt tattatccca tccaatcaat
     8041 ctgtcagaga taatccaaat cccatacgga tgacacggcg atgaaaaccc acacgcccac
     8101 gacccctctc aaggccctga tttcgggcat catcgggata gttatcttcc ttgtcggcct
     8161 cgtggtcctt aggttcatcg cacaccacac ttcatggccg ctctttgacg ggttcgtgga
     8221 cctcctcttt gcgcacgccg ctctcatcat cttcttctcg atacttttca caatcgggga
     8281 gatattcgct gcgttttcgt ttcccttcaa ccttccgttc ccggtcttca acgccgtggc
     8341 aagtgtgctt ttagtctctt tcctgatatc cctcctcgtg tatgtcaatg acttttacgc
     8401 catcggtatc gggcatgcgc tcggcgtggt ccggctcttt ctccttccgc tcacccttat
     8461 catcgtcctt gtcgcaggct acctctccat atttgtgaag atgaaaggcc ctgaagtgac
     8521 cccatcctca ccatctgggg ggagcacgga accaggtcgg tcctgtcctt cgtgggagac
     8581 tatcggggaa gagttccggc agatgatcgc ggacctgatc cggaagatca ggaatgagat
     8641 caacaaagat tgaggcggga tagagagatg aggggaaccc ttgagtgatc aggcccctgc
     8701 ggatatcccg agttttcttg ggagatcccg tgcccacacc ctgattgctt cccagtcccg
     8761 gaaatccccg acaggcccgt ccatcttctt ccatatccac ttgtgcatga acgggagctt
     8821 ctccatgtcg atttttccgg caaacatcgt cacatcgacg ggctccaggg gtgcaagagc
     8881 cgttcggaaa atattcatga ttgtgtcgac gtcgcccggg tcttttccga gcggtgcaac
     8941 ccctacgcag aatgcagcga ccggaacctg tacgagtgca tcccggtgct tcccgacgaa
     9001 ctttttcacg tcgcccacca tctttcccat ataaacaggt gccccgatga caactgcagt
     9061 ataatccccg ggagacgaca cggttttcat gacagcagta tcaacggtgt aaccctcttc
     9121 ctgcagtgct tttcctaccg cttcggcgac tcccgcagtc gatccatgca ccgatgcgta
     9181 tgcaactagt attcgggcgg gcatactcat tcctctccgg gatattgaga ggaagatctt
     9241 ataaagggtg ggggatgttc aaccataaga tccaattgct gttgtatggg cggccatcat
     9301 caggaataca ggacagtagg atttcagggg atcaccacgt ttttccttgg atcaccacag
     9361 tcattcaggg cgccatagaa aaaaaacaat accaagcctg gcagaaatat cgtgtatcag
     9421 atggccacct tcgtcctcgt ccagggaggc aacctctcca cagacacgtg gaataagctc
     9481 gccggaagat cagactaccc tcccggcgga caactcggag gggcgtactg gaacgggacc
     9541 gttgaggcac tcgccgcaca tggtcaccag gcattcgcac caacgctaga agatgaacac
     9601 aagactggcc ttgcagggca tatcaggcaa atatactcgc tttttaccgg aaactctctt
     9661 aaagatatca tcctggtcgg gcatagttac ggcgggatga tcatcacagg ggtagcggca
     9721 gcgatgcccg ggaggatcag gcacctggtc tatctcgatg cggcattgcc cgattcggga
     9781 cagtcgctgt tcgatctttt cgctctggca ggaagcgatc ccctctcttt tatcgggcta
     9841 gagcctgcga aagcctatac agtgaagata gaatttgacc cgcgtcacct cgaatccatc
     9901 ccgaagacct acatactttg cacaaagagc gaatttcgca tcgtcacagg acttgcgaag
     9961 cggaagatcg atgctgacag gaaagggtgg acctatctcg aactgccgtc gtcccacgtc
    10021 cctatggccg acatgccggg cgagttgctc aatattcttc tagatattgc tgaacggtaa
    10081 ccggcattgt ggagaggtgc ggcttcgcac tcttttttat tctcagccgg tatcctggat
    10141 tctcgatcgc ctctttacct cgacaagagt ggagtgaaaa acctgatttc acgccgcatg
    10201 cagcacaggt ttcaacttcc ggaaatgcag gatgaaacac agctggtccg aagaaatggg
    10261 tgaagcgtgg ccggggacat acctctccct ggactggagg aatggatgat gactggggct
    10321 tgtcccatcc tccttacggc cagagacgca tcaccagcag gtcctcgctt gcgtcgtgat
    10381 gggtgaaccc ggtgacgaca atctttccat cccgctggag cgcaatgccc tgcccgtagt
    10441 ccttgtggtc ccccggcccg ttccagagta cctgcccgcc attaccaaac gtcgtatcga
    10501 gtgttccatc agggtagaac cggacgacaa acacgtcatc gtaggttgcg ttctccgcat
    10561 atcctgcgac aattatcttc ccgtcctgct ggaccgcatg ggcatagcca tactcgtccc
    10621 tcccaccggc atcgccgtgg tggaccacgc ccccgatgcc gaatgcggga tcgggcgtgc
    10681 catcgggatt atatctcatc aggaggaggt cgaaagcatt atcgaccgat gtcgccccgc
    10741 tcacgagtat ctttccgtcg ggctgcacga gcacccagtt cccgtaatca aaattgtccc
    10801 cctccccgct gtagaggacc acccctccaa ccccgaacgt cccatccagc gtcccgtcat
    10861 ggttcatccg gaggaggagg agatcgtcct tttcgttggc cgcgctgctc ccggtcacaa
    10921 gtatcttccc gtcaggctgg agcgcacagg caaacaaacg gtccatgttt gtccccccat
    10981 aggtgaacag tccggtccct gcgaaccggg tatcgtagac cccttcaggc gtatagtgga
    11041 tgaccagggc atcctggtgg gtggcattcg cggtctcccc ggcaacaaag atgccgccgt
    11101 ccgactccca caccacgcca aagccgatgt cggtcgagga gcccggcgca ctgaacatga
    11161 ccaccccctc atttccaaag gtgggatcga ctgtgccgtt caaatcgtac cggagcagaa
    11221 gcagatcccg gtgactgcca ttctgggtga aacccgtggc aaccatcttc ccgtctccct
    11281 ggagcacaag gccaaggccc cggtcattgc cgccgccgtc gtaatatgca aagccgccac
    11341 tgccaaattg cgaatcgaga gcgccgtacg aggtataccg ggcaatcatc aggtcctcgt
    11401 tctgggtgtt gttgcagtac ccgagtgcaa cgatgtgtcc gtcatcctgt atcgcaacct
    11461 cgaccccacg gtccttctcg ttctctgcat gggtcgacat gacatacccc tggggagaat
    11521 tgaaggtgca atccagcgaa ccgggatgca gagtcggtgc gggcgactgg aaagccagga
    11581 gggccagtgc gcaaataaga agaaggatca ccagtccgga gaggacagcc accattctca
    11641 tactatacct ctttttaaag cattctgatg acagccctga taaagctcag gtccactcag
    11701 taccctaact ggatacctgg actggcggtg tcgctccggg tgaatcccgc attatgcatc
    11761 ggataagaaa tacttgccag gggcgatgtg attgcatccc ctctcttgcc gggtttcaaa
    11821 ggtcaatacc ccacagaccg ttaccatcat ggcttccagg gagcattctt ccgttaagaa
    11881 cccctttcga aagagatttt agcccccgga ataatcaccc cttatgaagt accccaccct
    11941 cgcaatcgcc atcctgctta ccttcctcat gctcgctgca ggttgtatga atactgcgcc
    12001 cccccagacc accggacccg gatccggcgt ctcgtcgcct gttgtcatcg acgcgcttgt
    12061 aacactcact ggtccgtatt cttctcaggg agcgatcgca aaagccgcct tgcttactgc
    12121 agaggaggat atcaaccggt attctgctgc catcggttcg gaacgccggg taagtgtcgt
    12181 gttccatgac accgcttcag accctgcgac cgcgcttaca caggccaaag aggtgcagaa
    12241 gagcggtcgg aggatcatcc ttgggcatat gaccagtgct gagatcgccg caatcaagac
    12301 attcgtcgat gcgaacggta tgttggtgct cgacgcaggg agcacgtctc cgtctcttgc
    12361 attccccgat gattcgatct accggctgat ttccgacgat tcagcacagg gcagggcaat
    12421 ggctatgtgg ctcgaagagc aacagataca ggcgattgtc cctctatgga ggggagatgt
    12481 ctggggtgat aatcttctca acgcgacacg gagtgcattt cttgaacgcg gtggggtggc
    12541 tatcgatggg attcggttcg gtccggacac cacggacttc tccttccacg ttgccagcct
    12601 tgatctcctt gcaggtgatg caatacaggc acatggtgcg gaccatgtcg gtatctacct
    12661 gattggattc gaggagagcg tccctatcct tgaggaaata tccgtaaaag agaacctcac
    12721 ccgtgtccgc tggttcgggt gcgacgggaa tgcagggatc ccgtcacttg caggtgtctc
    12781 ccctgcagcc agtgcggctg cacgggtcaa cctcaccagc gtcgtatggg gaattgccca
    12841 tggcgatcct tcaagcgagc ctcagacccg gatacgcgag cggctgggct actccccatc
    12901 gtccggaccc atcgcacttt acgatgccct ctgggtcgtg cacgatctcc tcaacgaaat
    12961 tccctccaat gcagatcaaa cccgccttaa actggccctt gagcggcacc tcaacaccta
    13021 ccagggtgag accagatcgc tcgcgacaaa cgagggaggg gacagggctc ttgcatccta
    13081 tgacgtattc caggtgatgg aacgagatgg gataccccaa tggaagtaca ttgcgcatat
    13141 cattatctgg gaagataggt ccgaacatat agggaccgat acctgccctt attttagaca
    13201 ggaataattc tgctgataac gcaaggacgg ggatcctcac ccatccctgc atctctctgg
    13261 gcagcgaggg agagaaagta tcaatcgtgg cggatgaaat ctataagaaa gaaacatctc
    13321 acaggaacgg catggcgttt cgactcacga cagaacggct gattctcgag gacttgaatg
    13381 aagacgatct cgacaacatc cggaggatcg catgcaataa ggaggtgatg cgctatgtcc
    13441 tcgtctggct cgaaaacgag gaccagatag ttggcttcct ccaacgggga atagaagagg
    13501 caaaacgacc ggaccggacc gcctatatcc ttgcggcaag ggtcgcggaa aacggtgagt
    13561 ttgcggggct cacattcctc gagattgacc cacagatcag gtgcactggg gaggtcggaa
    13621 tcgtcctcct ccctgaatac caggcatttg ggtacggaaa agagatcctt aaaacgtaca
    13681 tggagttcgg gttcgattcg ctctctctcc accgggtgtt cggcaaatgc gacgaaatga
    13741 accatgcctc ggctcgcctg atggagcagt gtggaatgac ctttgaaggc acaatccggg
    13801 agcatgtctg gctccgggac cactggcggt cgacacggta ttacgggatt cttgccgggg
    13861 aatatgcggc actcaacccc tgaaattggg tatagatgcg aaagtgatta gtatagtcga
    13921 tgagattgaa gggctctggc acttttcgaa ggaattgctg gtgataaaca tgaaatctga
    13981 cgaaatttcc tctccgggag atagaccctt atgcactgtc agctctcgta tcccatcctc
    14041 tggcagaagg tatctgttca tcaatctgcc atttccttca ggtatacagt gctctcgacg
    14101 gcccgtagac tctccggtat ataacaccgg taccggtctt cggcaagaac aaggggggtc
    14161 tctttctcaa tcccttttgt taagtcctgg aggattgtga cacgggcacc gcgggagagc
    14221 cacccctcca tcgaataccg gttacagtag tccgacgcca caccaatcag ggtgacctcg
    14281 acctctgccg ggatactgcc cgagaagaac gctttaagga actcatcccg gggaatgcat
    14341 tccgaagggg tatgtggatc atccaatata tgaaacaaat ttttatacac acgtcttttt
    14401 acaggatcgg tacactgcag agcccgtttc cggagattgg tccacatgct gaaattattt
    14461 ttcatcatgt agcggatatt cggagcccct ttcagatcga ttgcgagttc ccagtcccgc
    14521 gagccgtatt cacagtggat gggaaattgc cgggattcct gtgtttcgta gtattcctcc
    14581 ttgaagtgag tatcgagcac gatgagtgtg agatcaaaaa catcctcgct cacctgacgc
    14641 aaaaaatcat ttgcagggtc gatcaggttc tctgctcccg gaacatacag attcccttct
    14701 ttctgaataa agccgttctg catgtcgcct atttgaagaa ttgatacccg cttcatgata
    14761 tggttcatcc atgtattgta tcgggagtac tatttacctg atgattgatt gggagggatc
    14821 aatgcctttc caatcctttt ctcccgaggg gactttcagg gaggaggcat acgggatgga
    14881 ttaccggatc tggtgacctg atcagaagct tttggaatca cctgtgaagg tccgggcagg
    14941 gttgttaccc attcccatcc gaattcaatc caatcctcac gtttatcccc catgaacgca
    15001 agagcaaact ccatgggaga gatcatcgac gacccgctcc taagggagcg gacccgcgta
    15061 ttttccggcc gccatgatgc agggagaaga ctgggagtat tccttctctc gcttcctccc
    15121 accagcgaac ctcttgtcct ggcaattcct gcgggaggtg tgcccgttgg aaaggagatc
    15181 gccaagaccc tcggcgcccc gttctctctt gcaatagtga ggaaggtaag gatacccggg
    15241 acaactgaat ccgggttcgg agccgtggca tgggatggga cggtgctgat caacgagcgg
    15301 ctgcgggatg cgctcttcct ctccagttca gatgtcgaga gggctgtgag cgagacagcc
    15361 gcaaatgtga aagaacgtat cacgcggttc atggctggaa caccgatgcc gagtgttgcc
    15421 ggaaagaccg tgatcctgac cgacgacggg cttgcctcgg ggttcacgat gctcgccgcc
    15481 atccggagtc tcagggtacg agggcccgct cgcgtcgtgg tcgcggtccc tacggcatcg
    15541 cggtcctccg ctgagctcgt gggccgggag gcagatcaac tcgtctgcct gaacatccgg
    15601 tcagggaggt cgtttgccgt cgcggatgcc tacgaggagt ggtatgacct tgacgacagc
    15661 gaggtactcg cagaactcac cagcataccc cgctaaccag agacccgctg catattacac
    15721 ggttcctgga aggggaattg accctgcacc ttacgtgacg aatgagagta ggggtatgtt
    15781 caggaaagca ggggaagcgt tcctgcattc ccttcccatg gaaccgctca cttgcaggtc
    15841 ttcggggaga attccggcac catcaccatg acgctctggt tcgccgtccc cgtcagcgtg
    15901 atgttccggt cgtttcccac acaatagcac ctggccgaga tgataggcac cccggaacct
    15961 tcctggatga acgccacgta ccagccttcg ggtgcacgct cggtcctgat ggatttgacc
    16021 gggaatgtat ctgccgggta tcccttcagg tcaggatacg cctccttgat aagccggatt
    16081 gcttccctct cctcagggta gttccatctc ccataggcct cccaccagga tcttacctcg
    16141 tccttatcgg gatggaagta atgcagcgag cagagtgccg gggggtattt tccggcacga
    16201 tacccctcga tgagatagag cgcctcgttt ccgaccgtcg aagtctggtt gaacggcctg
    16261 taagagctca atggtgatac taccggtgct gcaggttcgg tggaatcgat ctcctgcatc
    16321 aggtgttcca cgtccgcagg ggtaatccag tcccggggta catcaatgac caggacatgt
    16381 gacctggggt gcatgcgaag gtcgtcaaga tactcaatcg gcgtatgcgt tgtgaaatag
    16441 caggagtctc cttgcgtcag gtgcacgtca gtctgtccgg taaggcgatc aaaggaacac
    16501 gcatccccgg actgccctgt gatatttgcc cagaaattgt gactcgccgc ccctcctcca
    16561 gggatattgc tgcacccggc agcggacagc aggaacgcga ggatcagaat gcccggaagg
    16621 attgctcgca ggctatgatt tgtcgaccgc gggatcgttg acatattcag gcctcctgaa
    16681 acaccttgag tcttctgatc aaaaaggtat tttcatacac atgccacggg gttctcgctc
    16741 ccgtcagccg agatccacgg gatggcaatt gctgattctt cagggaaaag tccggttttt
    16801 ttctcgctca ttaaaaagag gagaccttat atatactatt cagcctccat ctgcaattat
    16861 gactgatatc gcggtatttg tcgattcacg ggggggaaat acgaagaagg tggcggacac
    16921 cattgccgcc gagctgggtg ttgcggcggg agacccggcc gccatgctcc ctgcggatgc
    16981 aaaggtggta ttccttggga gcggggtgta tggcggaaag cctggaaaag ccatgaccgc
    17041 gttcatcgcg tcaggggact tctcggggct aaaggtggcc ctttttgcca catcgggtgg
    17101 tgctgccggg gctgataaga tgctcgggac gatggccgat gccctgaaag agaagggtgc
    17161 cacggtcatc gggaatttcc attgcagggg gaaatacctc ttcacgaacc gcggacaccc
    17221 ggggcccgaa gacctggaga atgcaaggaa gtttgcccgg gagatgcgtg caaattcctg
    17281 aaatctgggg agcgaaaaga ataccggcag aacatagagc cgaatctata tacaatctac
    17341 atggtgccat cacgatcagg tgaaaggcag actcgggttt tttggtacag ttgctggctc
    17401 tgtcgagctt gcaaccatag gttcccgcgt acttaatgga aagtgaatgg ggtttttcac
    17461 acgctgctgg cactgcgatc ttttcccttc tcaccccccg gcctcatccg ttgaataatt
    17521 caattattaa cactttgcag caaacatcag tctggtccaa acatgatcag gtccggatat
    17581 attttcttca ctttcatcgt cgccctggca atcctttccg ccgggtgcac gcagccttcc
    17641 gtcgaacacc aatctccagc cgcctcatcg gctgtgaata tcactgttcc tgttcccccc
    17701 atcccggtca ggagcagcgg cggtatgaat ctcgcgtacg aactggaact gacgcctgac
    17761 aatggtctca aattcgtgcc cgaggacgtc gaggtcattg accctgccac gggaaaggtt
    17821 ctctatatca tggagaagga actattcgca tccctgtatc accctgccgc agacccgctg
    17881 ccatctggta ctgaactcca gaacggcacg gggaaggtgc cggtcccgag gatctccatc
    17941 tggtttgtgg tcgatccgaa tgcggttccg gacaggctcg tccaccgggt cacgctcaac
    18001 atgagcggga gcgggcagcc cccgttcacc cagaccggcg gggagatcgc cgtgcgaaaa
    18061 gatctctccc ccgtggtcgt cggctccccg atgcgtggcc cgggctggct ggcactcgag
    18121 accaccgccc cggacaccca ccacttcagc gcccagatta caatggacgg agtgacccgc
    18181 gtcccgcaga aatacgcaca ggactggatc ttcgttgacc ctctcaccgg gaaggcggtg
    18241 gaaggtgact ctgccctagc caggaactac tatgggttcg gcaaggagat cctctcggtt
    18301 gcgaacggca cggtcgttga tatgctggac ggcctcccgg atcacgagat gatctattcc
    18361 ccacagggag tcacgatcgc aacggcggca ggcaactatg taatcgtgga cataggggac
    18421 ggaaaatacg cctgctatgc ccatatggtc aacggctcgg tccgggttgc accaggtgac
    18481 cgcgtcactg acggacaagt cctcggcctg atgggcaaca gcggcaactc ggacctcccc
    18541 cacctccact tccaggtggt ggaggggaaa gcctcgttcc tcggcgccga ggggtatccc
    18601 catgtctacc gggcattcaa tgtgacaggc gaggtcgacc aggcccgcgg cattgagatg
    18661 ttcagcgatc ctgaatttac cacagaaaaa ttatggtcag agtttgatat acttgtctcg
    18721 tcctacccta atcccgtgta ccagcaggag agcctcccgg aaaactgggt aatcctggac
    18781 ctcccctgat cgctaaaatt tttattgcat ctcacaagcg ggaatacttc atccggcggt
    18841 tacgctgaca ttcctgcttt ccaaccatgc cagttacgtc tccctggaca tccctcactg
    18901 cggaggggtt ccaggaaatc gaacgaacaa tttctggatt gcctccattc tgcaatcctg
    18961 acacgaatgt ctgaccgggc agccggaccg ccaatcaatc agttcccctt gacgacaagc
    19021 accgtgatgt cgtcgaactg gggcatatcc tttgaaaact cccgcacttc cgagaggatc
    19081 cggtcgagga tctcctgtgc agggaggcgg gcattttccc tgatgattgt gtagagccgg
    19141 tcttcaccaa agaacttttc ctgggcattc atcgcatccg tgactccgtc ggtatagatg
    19201 acgaccacat ctcctgtgcc gatagggatg gtacgggatg catactcctg gtgtgcgtcc
    19261 gccccgatgg ccatacctgt ggggaggagc tcttcaaggg tcccgtcacg gctcctgacc
    19321 acgacggggg ggttgtggcc ggcattcacg tacttcaggg tccggtcgga ttccgagagc
    19381 gccccaaaaa agagggtgac aaacatgccg gactttgagt cttcggtgat gatgttgttt
    19441 gcatcgaata tcgctttcgc agggtcccgg tgccagagcg cattcaccct caccacgatc
    19501 cg
//
LOCUS       DAOQYR010000038        18850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_3316_length_18850_cov_7.257356, whole genome shotgun sequence.
ACCESSION   DAOQYR010000038 DAOQYR010000000
VERSION     DAOQYR010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 18850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18850
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3316_length_18850_cov_7.257356"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            446..2152
                     /gene="kdpA"
                     /locus_tag="PKM87_06100"
     CDS             446..2152
                     /gene="kdpA"
                     /locus_tag="PKM87_06100"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HNQ29674.1"
                     /translation="MPAANPVFDVTLFILFLIIVTGAAVLAGRYLAAVFSGSLTWAPF
                     SQIEHALFRLVGTSPEQEESWQGYARDLLIFNALGFLILFLILLLQGILPLNPQQVPG
                     FFLIDALNIAVSFVTNTNWQVYSGEASVNYFTQVLGLTVQNFLSAATGICVAIAVMRG
                     HTRKMTDRIGNFWVDMTRAVLYVLLPLACIAALILLSQGVIQNFDPSLKTTGYGGTGS
                     QTIAMGPVASQEAIKELGTNGGGFFNANSAHPYENPTSFSNLFEIFLILLIPAALPFT
                     FGIISGQKRQGWALYAVMMMLFVASLGTMYASELSGNPSRALQDVPGVSMEGKEVRFG
                     LSGTALFSTSTTATACGAVNAMHDSLTPLGGLVPMFLILLGEVVFGGVGSGFATMIAF
                     VIVAVFIAGLMIGRTPEYLAKKVEIFEMKMALIAILIPGILVLVFVGIGVVIEGGLLG
                     IFNPGPHGLSEIVYAFASMANNNGSAFAGLNAALPFYTLSGAVVMVIGRYVPAIAMLA
                     LAGTMARKKTIPPGPGTLPTASPSFVLWLIAVILIIGALTFFPLFAMGPIAEHFALAG
                     GL"
     gene            2154..4223
                     /gene="kdpB"
                     /locus_tag="PKM87_06105"
     CDS             2154..4223
                     /gene="kdpB"
                     /locus_tag="PKM87_06105"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HNQ29675.1"
                     /translation="MARKRVTPVTAFDAALLRDASVSAIRNLDLRRLCKNPVMFSVEI
                     GGILTTLIFLYETFVPAGGSVFFVGMVSAWLWLTVLFSNFAESLAEGRGKARAASLRR
                     SRATMTANKIESQSLSAPVTTVASPDLRCGDLVLVRTGDLIPSDGEVIAGAALVNEAA
                     ITGESAPVVREAGGDRSAVTGGTTVIANEIIVRITAEPGKTFLDCMIQMIEGATRRKT
                     PNEIALEILLFALTGIFLLVVATIWPVSAYSAAVSGSGSPVHVAVLVALFVCLAPTTI
                     AALLPAIGIAGMDRLFQRNIIAMSGRAIEAAGDVNILLLDKTGTITLGNRQAVAFIPV
                     DGMADEDLVTAAYCASYDDETPEGKSIISLACASGCPTTCPPEHAVFIPFSAQTRMSG
                     MDIGGTTYRKGATDAITNEVTSAGREVPPDLVSKVTRIAQTGGTPLVVSKDHSVIGVI
                     HLKDILKSGVRERFFDLRRMGIRTVMITGDNPLTAAAIAAEAGVDDFLAETKPDDKLR
                     LIKEYQDEGYMVAMTGDGTNDAPALAQADVAVAMNNGTQPAREAANIIDLDSDPTKLI
                     DVVEIGKQILMTRGALTTFSIANDVAKYFAIIPAAFLVIYPQLAVLNIMGLATPESAI
                     ISAVIFNALIIPLLIPLALRGVQFRPMPAARLFTYNLVIFGLGGIVAPFLGIKGIDIL
                     ISAVMPL"
     gene            4220..4798
                     /gene="kdpC"
                     /locus_tag="PKM87_06110"
     CDS             4220..4798
                     /gene="kdpC"
                     /locus_tag="PKM87_06110"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HNQ29676.1"
                     /translation="MTWQRPLRTAILLFCILFFITGIVYPLFVTCVAELAFPSQAHGS
                     LVRNGSGAVMGSELIGLPFTGPHYFEGRPSSTPGSPYNATRSGGSNLGPSNPALLVKV
                     KERIRHLETLGISGPWPGDLVTSSGSGLDPHLTIDAALLQVPVVAHARGMDEDAVMAL
                     VYQNAVPDPLSQGHPYVNVFALNRALDEHGVP"
     gene            4795..6735
                     /locus_tag="PKM87_06115"
     CDS             4795..6735
                     /locus_tag="PKM87_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HNQ29677.1"
                     /translation="MTGDWDKDRARPAPEDLLPLANAEERAGHRGKLIIYLGYAAGVG
                     KTYTMLWDALQRNTEGTDVVIGYIETHKRIETDALAASLETLSPQQVWYQGLSISEMD
                     LDAILNRKPSIVLVDELAHTNGPGTRHLKRYHDIEEILRAGISVYTTVNIQHVESLND
                     AISQITGIQVRETVPDTFFDLADDIRLIDLTPEELIARLKAGKVYLGDMAAQAVDHFF
                     STGNLIALRQLTLRYLAQRTDRQMVTHMRARAISGPWPAVERLLVAIRPGPTAEAMVR
                     AAFRDSQRLNTEWVVLSVEEEREGPPTPQEKAWLSDAMDTARKLGGKILIYRGEDVAH
                     EIIRYARHHNVTTIMLGKPRGLDILISPVYRIMRTSPGIDIILFDPKGAPSIPLEQQI
                     PHLLPRDYLITLVMILTVTLLNHLLLGTISTANLIIIQLLPVVASAWFFSRKASLFAA
                     VVSILAFDFAFVAPYFTLNISDWEYFISFAGYVVIAIIISHLANRLRYLIPQIRESEA
                     RVAAVAGLSKDLVDTESYQEVLEILTTHMRAFAPGKVAILVPDDHGIRVATGDREYPF
                     SDKELTIARWVYENGEVAGQGTETLVGGTGHYVPMKAHGLVYGVLAFAFQNPDAVLSL
                     ETKEVPEAMAQLGALALERVMK"
     gene            complement(7226..9736)
                     /locus_tag="PKM87_06120"
     CDS             complement(7226..9736)
                     /locus_tag="PKM87_06120"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNQ29678.1"
                     /translation="MNPHRTLIILFLLLALCACCLPPAAAILPSYCFRGTVTDLSRSD
                     NTVTVLVTHKWGCEYFDAAATCNWKSISPQILSGTAPTPEVFDYVRIGSSVEAGSIGS
                     QGERWVGIGVLTPSYSSEPLHATRLFGDLGILRAPLASRYSLAVFTQPDCGECAGAVC
                     VAQSTNVTISRDGLEMLAVTLLPGEEVICTDPADGSGVTLTFVSGQASVNLCPDTPPG
                     MSGVQPISVFVVHVNQGGPGPSPTRTPVSTGFLSVSSIPSGATIILDGVSKGSTPRTI
                     SGIEPGVHALVLEKDGYAPYERDVTISAGRPTTVTATLTATYGSLRIQSSPSGAAVLF
                     NGTAIGSTPLVANDLEPGTYAVSLSKTGYETANRTVAVTPGKEQLLFVALSKKADGSD
                     KIDAFIAALQDEGFTVQQGKFEAFDVLAMYDAGVIPSCYGNNPSTPYLVYKLPGYPGL
                     AQGGRVSDAPIRPDNKGLWLDYFMEPNEAIVFVGSTPPEVKYYSYRSYIGTRWFTELD
                     DFERIFASLGDTVNNYRINVGTTPGQFTPGPYEKPVMIITTGDMGTNDLVRKAALKAG
                     YPTGIINTDIIPSGLIRMGLSNTSDTITFIHRLAFFANETRGNEYLNGTPGYVFRLTP
                     NTPHTQQPYGVPRLIVRGTGDTHELDLLKTQEALRDAIVARYGNGMVATANESYIWIL
                     EGYDSLQRESDALGDNRDAFYLRNGDYLLSDDDFVIVYGVNHQATGKVVYTNIAVYGT
                     EALNGVVAVTDADIAGSANSYLPGNPNAGLFYVWKFARHCNGEVGCTEIPYCCGGLGI
                     PADVPIRIGYRTYVEPGTGIGPTCTEILYDQAIHFSPV"
     gene            10509..10808
                     /locus_tag="PKM87_06125"
     CDS             10509..10808
                     /locus_tag="PKM87_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29679.1"
                     /translation="MKRKVAGILALLLVATLFLAPAAAGFVTPSKPGLSGNSLDYFTP
                     VSPGNLPGYAAQWRSLDGINPNEPSTMADLASEIRNMRYGPSPQRPFSWTPSCTI"
     gene            11054..13591
                     /locus_tag="PKM87_06130"
     CDS             11054..13591
                     /locus_tag="PKM87_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNQ29680.1"
                     /translation="MASTRPDTKYLATRSQEEVLAQLGSSLARGLSADEVSRRLEKEG
                     YNEITANQVRWWEIALRQFRSAFIYLLLVAAVIVILIGEYVDGVVILGFVLVNALLGF
                     YQEYRSEQALQALQQFITPRTRVLRESRWEVRASRDLVPGDIIRLTTGDVVPADVRVL
                     TSQNLVVNETVLTGESVPVPKLPDTLDAVPVDVYGSHNLCFSGTTVVGGEAVAVVLAT
                     GAQTAMGTIAKMTVETTKESEFSKGIGRFSQFILRMILVTLVFVFVANILLKGGSVDL
                     VGLVVFSIALVVSVIPEALPVVTTVSLSRGALRLAEHDVVVKRLSAIEDIGSIDVLCS
                     DKTGTLTENILTVTGLSPGADPALLSYALLTVAETEEKTEPFDIAIVDAAARLSPGEI
                     LASERLAEIPFDPKTRWNAVLVSLPAGPVLVIRGAPEEILRLSPSLPDAGRMAVADWL
                     SRNGLAGERTIAIAKKALPPGKTGISPGDVRDLEFLGAMAFTDPIKPSAFRAVEMARE
                     LGVALKIITGDSPEVAGAVGVRIGLLKDPAAVVTGSALAAIPPGEREQVIQEHAVFAR
                     ITPEQKFEIIETLQRTCTVGFLGEGINDAPALKRAGVSLVVDSAADIAREAADIILLQ
                     KDLEVIIEGIRSGREVFANSVKYLKATLASNFGNFYAIAIVSLFIPFLPMLPIQILLV
                     NLLSDFPMISIATDTVDEAEFRLPGRYDLKEIVLIATLLGVISTVDDFAFFAVFVGDG
                     AQVLQTSWFIGSILTELVFLFSIRTRLPFYRARRPSIHVLALTTVAAAATILLPYTSI
                     GQALFSFVPPSPGALAIILGLVAAFFVTTELVKVSYYRYAGGLPARS"
     gene            13967..17473
                     /locus_tag="PKM87_06135"
     CDS             13967..17473
                     /locus_tag="PKM87_06135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ29681.1"
                     /translation="MISVLFVDNEPHFCELVKSTLEQSGLFSVDTAESASAALGKMKH
                     GSYDAIVSDYQMPDMNGIEFLKALRASGNEIPFIIFTGKGREEVVIQAYENGADFYVQ
                     KSGDPEVQYADLAQKITRAVGLHRAEHDRLESEDRLRQIIQFLPDATFAIDMEGRVIA
                     WNKAIEEMTGAPASQMIGKGKYEYAIPFYGERRPILIDLAMQPDEETTSRYAFVRKEG
                     RFLEAEASNARLQGKDVVLWGKATPLYDTRCRLTGAIESIRDITGFKKTEETLRESEA
                     RYRRFAENAKDLIYRYTFTPIRGFTYVSPSSTNIVGYTPEEHYADPDIGWKLVFEDDR
                     HLLESLAHDPGVYKKPLVVRWKKKDGTVIWTEQINHPIYDNEGNIIAVEGIARDITDR
                     TRSEEAIREKQIQLANAMDLSHLVMWEYDVASDMFTFNDNFYSLYGTTASQEGGYQMS
                     SKEYAARFVHPDDVHFVAEEVQNALKSTDPSFSSQLEHRIVRRDGEVRFIIVRIAGVA
                     GESGTLIKTYGANQDITDRIKAETTLRESEARYRTFVENAAEGVVVIQDGKIVYANPA
                     ALLLTRESLENLLEKPFGQYIHPDDRDMVVGRYKRRMLGEDLPSIYDFRLAGTDGYET
                     WVQISVAVIPWNGRPATLNFLSDITERKQREQLLQIQHRIATLCSSPAPLSEILPECM
                     HHVLALTRMDAGGIFLVDEHSGLSLVYSTGLSEAFVREEQYHMQDSPQTEFVMQGRPY
                     FGDFSSLQFPPSPARASEGIRALAVVPFVHQGRVTGCLNLASHTRETFPQVIQEAIST
                     LAIQMGSDISRIQASEALRKSEEKFRRMVETAAEGIWEMDQDFHITYANQRMADLLGY
                     TREEMVGREISTFMDRDELPDSLARKERQRQGIADHFERKLIRKDGMPLWIYSSVTPV
                     FDADGRFRGSFAMYSDITDRKLIEAEIRSLNQVLEQRVGERTAELTRLLHEREVLIRE
                     VHHRVKNNLQIIISLLNLQGKYITDQKVLAAIRESQNRIKSMALVHERMYQSEEVSRI
                     TFREYVRFLVSHLFTFYDVRAYQVSYTITMDPFPIDIDTAIPLGLIMTELVSNSLKHA
                     FPAGRKGTISIEGRKVDGHTFRFVVKDNGVGISPEIDWKNSDTLGLRLVKSLVTQLNG
                     TIELDRADGTTFTLHVRAKEAK"
     gene            17704..>18850
                     /locus_tag="PKM87_06140"
     CDS             17704..>18850
                     /locus_tag="PKM87_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29682.1"
                     /translation="METETYDKDKVVFAATMASTFEPDSMTNDKIEAATRGHGALVIP
                     VFAAANSIAEDLFCPITGPEMSLMGRMTVVDASVGELPLDVVIQKAVRAAMSAGAAPE
                     NAALIVASLAYFSGSCARSGVPLGNRKLGAIARMHAGAARTSAIALVTGKSTHRIQSF
                     PAYLAIYEAMMDKKLTKVDGSMLPPFISGGAIYGHSALGEDYNIPELAYNAAHAGTLA
                     MMKSMAGTGITPLALWPALIGAAVTMEIVHPDALLGEEFGKFGRVDSSHLAGKGARDA
                     AKLPEKIHIRGTHEEYDTAKIIGEFGLILKDIGGPSVIGSMALSEIFAAFEEAPMIGA
                     GFSGGPVNPPLGHLEGDCVPALRLLMKHHGDIFAVAEVIRDYKMNSFI"
BASE COUNT         4066 a         5303 c         5328 g         4153 t
ORIGIN      
        1 caatattttt cgttgccatt ctcttcacct ctctctactc cctcccttga ttcacttttg
       61 cgttcagatt ctcctctcgg ttcctgaaca agagggaggc ctccagtttt aagctgggtg
      121 gaaggaaata gttttccttg tatattaaga tatttgtatg gaagaaccac cagaatttcc
      181 ttcagtgctt ccagcttttt gacgggagct gaataaacct ttgatgaaaa accaagactt
      241 tcttttaatg tgaggtccct tgggactata tatgattatt aatagtatta ttcagttcac
      301 aatccagcac ttattacttt aatttatcaa tagtatttcc cggtatttcg ctggctcaca
      361 gacaagttat taaacaatga attaggaagc atccttctaa ggaacatgga atcaccatca
      421 accagagatt agagggagcc ccccgatgcc agccgcaaat cccgttttcg atgtaaccct
      481 gtttatcttg ttcctcatca tcgtgactgg agccgcagtc cttgcggggc gctacctggc
      541 agcggtgttt tccggatcgc tcacatgggc acctttttcc cagattgaac atgcactctt
      601 tcggctggtt gggacttccc cggaacagga agagtcctgg cagggatatg cacgggacct
      661 gctcatcttc aatgcacttg gatttcttat tcttttcctc attctccttc ttcaggggat
      721 attaccgctc aatccccagc aggtccccgg attttttctc attgatgccc tgaatattgc
      781 cgtgagtttt gtgaccaata ctaactggca ggtatacagc ggcgaagcat cggtgaacta
      841 tttcacccag gttctcggcc tgaccgtaca gaatttcctt tctgcagcca ccgggatctg
      901 cgtggcgata gccgtcatgc gggggcatac caggaagatg accgacagga tcgggaactt
      961 ctgggtcgat atgacccggg ccgttctcta tgtcctcctc cccctggcat gcatcgcagc
     1021 actcatcctc ctttcgcagg gagtgatcca gaatttcgat ccatcactca aaacgacagg
     1081 atatggaggg accgggagtc agactattgc aatgggccct gtcgcctcgc aggaggcgat
     1141 caaggagctc ggaacgaatg gaggaggatt cttcaacgcc aactccgccc acccgtacga
     1201 aaatcccaca tcgttctcca acctcttcga gatattcctt atccttctca tccccgcggc
     1261 attgccgttc accttcggaa taatttccgg acagaagagg cagggatggg ctctctatgc
     1321 agtcatgatg atgctctttg tcgcatcact ggggaccatg tatgcctctg aactgtcggg
     1381 gaatccatcc cgcgccctgc aggacgtgcc cggggtatcg atggagggaa aggaggtcag
     1441 gttcgggctt tccgggacgg cactcttcag cacctcgact actgcgactg cctgcggggc
     1501 cgtgaacgcc atgcatgatt cactcacccc gcttggcggg ctggtcccca tgttccttat
     1561 ccttctcggg gaggtagtct tcgggggcgt agggtcaggc tttgcgacca tgattgcctt
     1621 tgtaatcgtt gccgtgttca ttgcgggcct tatgatcggc cggacccctg agtatcttgc
     1681 aaagaaggtg gagatcttcg agatgaagat ggctcttatc gccatcctca tcccgggtat
     1741 tctcgtactg gtctttgtgg gaatcggtgt agtgatcgag ggaggccttt tagggatctt
     1801 caatcccggt cctcatggtc tttctgaaat tgtctatgca ttcgcatcga tggcgaacaa
     1861 caatggaagt gcctttgcag gactcaatgc cgcactgccc ttctacactc tctccggggc
     1921 agtcgtcatg gttataggga gatatgtccc cgcgatcgct atgctggcgc ttgcgggtac
     1981 catggcccgg aaaaagacta tcccccctgg accagggacc cttcccacgg catctccctc
     2041 attcgtgctc tggctgattg cagtgattct gattatcggg gcgctcacct tcttcccact
     2101 gttcgcgatg gggcccatcg cagaacactt cgctcttgca ggggggctct gacgtggcgc
     2161 gaaaaagggt gacgccggtc accgcctttg atgctgcact gttgcgggat gcatcagtca
     2221 gtgccatcag gaacctcgac ttgaggcgac tgtgcaaaaa tccggtcatg ttctctgtag
     2281 agattggcgg aattctgacg acactcatat ttctatacga gaccttcgtc ccggcaggag
     2341 gttccgtctt ttttgtcggc atggtctcgg cctggctctg gctgacggtg ctcttctcga
     2401 attttgccga atcgcttgca gaagggcgtg gaaaggcacg agcggcctcg ctccggcgat
     2461 caagggctac catgactgcg aataaaatag aatctcaatc cttatcagcg ccagttacca
     2521 ccgtggcgtc accggatctc aggtgcgggg acctcgtcct tgtaaggacc ggcgatctta
     2581 tcccgagcga tggcgaagta atcgcgggag ccgctctcgt caacgaggca gcgattaccg
     2641 gtgaatctgc tcctgttgtc agggaagccg gaggggacag gagtgcagta accgggggga
     2701 ccacggtgat tgccaacgaa atcatcgtcc ggatcaccgc ggagcctgga aagactttcc
     2761 tcgactgcat gatccagatg atcgaaggtg caacccggag gaagaccccg aacgaaatag
     2821 cgctcgagat cctactcttt gctctcacag gtatcttctt gctcgtggta gcgaccatat
     2881 ggccggtctc tgcctacagt gcggcggtgt ctgggagcgg gagccccgta catgttgcag
     2941 tcctggttgc cctctttgtc tgccttgcgc ccaccaccat tgctgctctt ctccccgcga
     3001 tcgggatcgc cgggatggac cgcctcttcc agagaaacat catcgcgatg tcggggaggg
     3061 cgatcgaggc ggcaggggac gtgaatatcc tgctgctcga caagaccggc accatcacac
     3121 tcgggaaccg gcaggctgtc gcgttcatcc cggtcgacgg catggccgac gaggaccttg
     3181 tcactgcggc atactgtgca tcctatgatg atgagacccc ggagggaaag agcatcatct
     3241 cccttgcctg tgcatccgga tgtcccacta cctgtcctcc tgaacacgcc gtgttcatcc
     3301 cgttctcggc gcagacacgc atgagcggga tggatatcgg cgggaccact taccggaagg
     3361 gtgccaccga tgccatcacg aacgaggtga cctcggcagg aagagaggtc cccccggacc
     3421 ttgtttcgaa ggtgacccgg atagcccaga ccggcggcac tcctcttgtc gtgagtaaag
     3481 atcattcggt catcggggtg attcatctca aggatatctt aaaatccggt gtccgggaga
     3541 ggttcttcga cctccggagg atgggaatca ggacagttat gatcacaggg gataaccccc
     3601 tcacggcagc agcaatcgct gccgaagcgg gggtcgatga tttcctggca gagacaaaac
     3661 ccgatgacaa gctccggctg attaaggagt accaggatga agggtatatg gttgccatga
     3721 caggcgacgg cacaaacgac gccccggcgc ttgcccaggc agatgtcgcg gttgccatga
     3781 acaatggaac ccagccggcc cgcgaggctg cgaatatcat cgatctcgac tctgacccga
     3841 ccaagctgat cgatgtcgtg gagatcggta aacagatcct gatgacccgg ggggccctca
     3901 ccacgttttc cattgcaaac gatgtggcga aatacttcgc catcatcccg gcggcgttcc
     3961 tagtcatcta tccgcagctc gcagtgctca atatcatggg actggcaacc cccgagtccg
     4021 ccatcatctc tgcggtcatc tttaatgccc tcatcatccc actcttaatt ccccttgccc
     4081 tccggggagt acagttccgg cccatgcctg ctgccaggtt attcacatac aaccttgtaa
     4141 tcttcggact cggcggaatc gtggcaccat tccttggaat caagggtatc gacatcctca
     4201 tctcggcggt gatgcccctg tgacctggca gagacctctc cggacggcaa ttctcctgtt
     4261 ctgtatattg ttcttcatca ccggcatcgt gtacccgctc tttgtgacgt gtgttgccga
     4321 gctcgcattt ccttcccaag ctcatggaag ccttgtcagg aacgggagtg gtgcagtgat
     4381 gggatctgag ttgattgggc ttcccttcac cggcccgcac tattttgagg gccgcccctc
     4441 ctcaacaccg ggatctccct ataatgccac ccgatcaggg ggatccaatc tcggcccctc
     4501 gaatcctgcc ctgctggtga aggtgaaaga gagaatccgc caccttgaaa ctctcgggat
     4561 ttcggggccc tggcccgggg atctcgtcac atcctcaggg agcggccttg atccacacct
     4621 caccatcgat gccgctctgc tgcaggttcc tgtcgttgcg cacgcacgtg gaatggacga
     4681 ggatgcagtc atggctctgg tgtaccagaa tgcagtaccg gatcccctct cccaggggca
     4741 tccttatgtg aacgtctttg ccctcaacag ggcacttgat gagcatggtg tcccatgacc
     4801 ggcgactggg acaaggatcg ggccagaccg gcaccggaag acctgcttcc tcttgcaaac
     4861 gccgaagagc gggctggaca tcgcgggaag cttatcattt atcttgggta tgctgcaggg
     4921 gtcggcaaga cttatacaat gctttgggat gccctccaga ggaatactga ggggacagat
     4981 gtggtcattg gctacatcga gacgcacaag cgtatcgaga ccgatgcgct tgccgcgtcc
     5041 ctggagacac tttctccgca gcaggtctgg taccagggac tctcgattag cgagatggac
     5101 cttgatgcca ttctcaacag gaaaccctct attgttcttg tcgatgagct ggcgcacacc
     5161 aacggcccgg gtacccgtca cctgaagcgc tatcacgata ttgaagagat actaagagcc
     5221 ggaatctcag tctataccac agtgaacatc cagcatgtcg agagcctgaa tgatgccatc
     5281 tcccagatta ccggcatcca ggtcagggag acagtccctg acacgttctt cgacctcgcg
     5341 gatgatatcc ggctgatcga cctcactccc gaagagctga ttgcacgcct gaaagccgga
     5401 aaagtgtatc ttggtgacat ggccgcccag gcagtggacc acttcttctc aaccgggaac
     5461 ctgatcgctc tccggcagct gacgctcagg tacctcgccc agcggaccga ccgccagatg
     5521 gtgacccaca tgagggcccg ggccatttca ggtccatggc ccgcagtcga gcgcctcctt
     5581 gttgccatcc gcccgggccc taccgcggag gccatggtca gggcagcatt ccgggattca
     5641 cagcggctga atactgaatg ggtggtactc tctgtagagg aggagcggga agggccaccc
     5701 actccacagg agaaggcctg gctctctgat gccatggaca ccgccaggaa actaggggga
     5761 aagatcctga tctaccgcgg ggaggacgtc gcccacgaga tcatcaggta tgctcgccat
     5821 cacaacgtga ccacgatcat gctcggcaaa ccccgcggcc tggacatcct tatttcaccg
     5881 gtctatcgga tcatgcgcac ctctccaggg atcgacatca tcctcttcga cccgaagggg
     5941 gcgccgtcga tcccacttga gcagcagatc ccgcatctcc ttccaaggga ttatctcatc
     6001 acccttgtga tgatccttac agttacactc ctgaatcacc tgctgcttgg gacaatcagc
     6061 acggcaaacc tcatcattat ccaactcttg cccgtcgtcg cgagtgcctg gttcttctcg
     6121 cggaaagcct cattattcgc ggcagttgtg agcattctcg cctttgattt tgcgtttgtt
     6181 gcgccctatt tcacgctgaa tatctctgac tgggaatatt tcatctcgtt tgccgggtac
     6241 gtcgtcatcg cgatcatcat cagccacctc gcaaaccggc tccggtacct catcccccaa
     6301 atcagggaga gcgaggccag ggttgctgcg gttgccggtc tttccaagga tctcgttgac
     6361 acagagagtt accaggaagt cctggagatc ctgacaacac acatgcgggc ttttgctccc
     6421 ggtaaagtcg caattcttgt cccggacgat cacggtatca gggtggcaac tggtgacagg
     6481 gagtatcctt tttcagacaa ggagttgacc atcgcccgct gggtctacga gaacggggag
     6541 gtcgctgggc agggcaccga aaccctcgtc gggggaactg gtcactatgt gccgatgaaa
     6601 gcccatggcc tcgtatatgg tgtactggca tttgcattcc agaatccaga tgccgtgctc
     6661 tccctggaga caaaggaagt ccctgaagcg atggcccagc tcggggctct tgcccttgaa
     6721 cgggtgatga agtaaataga agggaagggc gaaggaccgg gtgcagggta taaggtatcc
     6781 acactgccga cggcttctcc gcggctttcc ccagaattcc tatgcgcctc ctgcactgct
     6841 cgcagaactc cggaccctcc aggtcagtgt ccctgatcta ttaggagaag tagatgacgc
     6901 aagcccggat actcgcagtg gggggaggcc gggcgcaagt cacttatcga taatccgtac
     6961 ccggacttga ctgtgacgtc accagtccgt cttgcccttg ccaccgatgt acgcatctcg
     7021 cagtccttga aggtcaatga agtcttcccc ccaaattgtg cgaatatcaa catgatgaag
     7081 atactgggca tctccccgca gccccgtcca gcgcctagat cagaaactcc cccgactgct
     7141 cgtcagtgag gaggagctgg ttggaatctc gctggtcgat caattcacaa tccaacattt
     7201 acgaaaaaag gccgggatgg atctctcaca ctggagagaa gtgaattgcc tggtcgtaca
     7261 ggatctcggt gcaggtcggc cctatccctg tccccggctc gacataggtc cgatacccga
     7321 tgcggatcgg aacatccgcc gggatcccga ggccgccgca gcagtacggg atctcggtgc
     7381 atcccacctc gccattgcag tggcgtgcaa acttccagac atagaagagg ccggcattgg
     7441 gatttcctgg gaggtaagaa ttggcagaac cggcaatatc ggcatcagtg actgcaacta
     7501 cgccgttcaa tgcttcggtc ccgtagaccg cgatgttcgt gtagaccact tttccggttg
     7561 cctggtggtt gacaccgtaa acgatgacaa aatcatcgtc agagaggagg taatcaccgt
     7621 tccgcaggta gaatgcgtcc cggttatccc caagagcgtc ggattcccgc tggagtgaat
     7681 catacccttc aaggatccag atatacgatt cgttcgcggt tgcgaccata ccgtttccgt
     7741 acctggcgac gatggcatcc cggagtgcct cctgtgtctt caggaggtca agctcatggg
     7801 tgtcgcctgt ccccctcacg atgagccgcg gcacaccata gggctgttga gtgtgcgggg
     7861 tgttcggtgt aagcctgaag acatagcccg gagtgccgtt cagatactca ttcccccgtg
     7921 tctcgttggc aaagaaggcc agtcggtgaa tgaatgtgat ggtgtccgaa gtgttcgaca
     7981 ggcccatccg gatgagccct gaagggatga tatcggtatt gattatccct gtcgggtacc
     8041 ctgccttcag ggcagccttc cggaccagat cgtttgtacc catgtcaccg gtagtgatga
     8101 tcatcacggg tttttcgtag gggcccggtg tgaactggcc tggtgtcgtg ccgacattga
     8161 tgcgataatt attgacagtg tcgccgagac ttgcaaatat acgctcgaaa tcatccagtt
     8221 ctgtgaacca gcgggtcccg atataactcc ggtagctgta gtatttcacc tctggagggg
     8281 tggatcccac gaagacaatt gcctcgtttg gctccatgaa gtaatccagc cagaggcctt
     8341 tgttatctgg cctgatgggt gcatctgata ctcttccccc ctgggcaaga ccgggataac
     8401 ccggaagctt gtagacgagg taaggggtgc tcgggttatt gccgtagcag ctcgggatga
     8461 cgccggcatc gtacatggcg agcacatcga aggcctcgaa cttcccctgc tggacagtga
     8521 agccctcatc ctggagtgcc gcgatgaacg catcgatctt gtcggatccg tccgcctttt
     8581 tggacagtgc gacaaaaagg agctgctcct ttcccggcgt cacagccacc gtgcggttgg
     8641 cggtctcgta ccctgtcttc gagagggaga cagcatacgt tcccggctcg agatcattgg
     8701 cgacgagagg ggtagacccg atggcggtcc cgttgaagag gacagctgcc cctgacggtg
     8761 aggactggat ccgcaggctc ccataggtag ctgtgagggt cgcagtcact gttgtcggtc
     8821 tcccggcaga aatggtcacg tctctttcgt atggcgcata cccgtccttt tcaaggacaa
     8881 gggcatgcac ccctggttcg atgccggaga ttgtcctcgg ggttgagcct tttgacaccc
     8941 cgtcaaggat gatggttgcc ccggaaggga tcgaggacac gctcagaaac ccggtggaga
     9001 ccggggtcct cgtcggactg ggccccggtc ctccctggtt gacgtggacg acgaagaccg
     9061 agataggctg gacccccgac atcccgggag gcgtgtcagg gcagaggttc accgatgcct
     9121 gcccggagac gaacgtgagg gtgacgccag acccgtccgc agggtccgtg cagattacct
     9181 cctctcctgg gagaagcgtc acggccagca tctcaaggcc gtcccgggag atggtgacgt
     9241 ttgtggactg tgccacgcat accgcaccag cgcattctcc gcagtccggt tgtgtaaaga
     9301 ccgccaggct gtacctggat gcaagggggg ctcgaagaat gcccaggtcc ccgaagagac
     9361 gggtcgcatg gaggggctcg gaggagtacg agggtgtcag gaccccgatg ccgacccatc
     9421 gttcaccctg cgacccgatg ctccctgcct ctactgatga tccgatcctg acataatcaa
     9481 agacctcggg agtgggtgcg gtgcccgaaa ggatctgcgg gctgatcgat ttccagttgc
     9541 atgtcgcggc ggcgtcgaag tactcgcagc cccacttgtg tgtcacgagg acagtgacgg
     9601 tgttgtctga acgcgacagg tcggtcacgg tcccccggaa gcagtatgac gggaggatag
     9661 ctgccgcggg ggggaggcaa caggcgcaga gggccagtag aaggaagagg atgatgagtg
     9721 tacggtgtgg attcatagaa acatctcctc cggattgatc cgggtttgat ggagaaccat
     9781 ttgcgaggaa gtatataggt gtattgaatt tttatgagga gtggggattt ggggagaacc
     9841 agaggacagg tcgtggactg ctcctgcagg aaacggaatg gattcacaag gccaggtcgg
     9901 gatgcgtggg tgtgatgcca tgcaacacgg tgcgggaccc tgtccttccc tactcagtcg
     9961 attacggggt gtgttccggc gaggatccgg gaggatggcg gcaattttcc gacgaaaatc
    10021 atcacacttt tttttaatgt ccatacaggg agctcaaatc gaaacactct tgctttcagc
    10081 agttatacca gttgatactg cagtgggcat ggaggtatac ctattggctg ctgcaggaat
    10141 aaccggacaa aaagggtgga tctatcctcc ccacactgcc ggatacgcct ggtgtgtccc
    10201 cccttacacc aggacggcag tcctttctct ctatttccca cacgcgatgg gaagagaatc
    10261 tccgaaggaa gagtccgggg tgtcggcgtt ccggacccgg gagggccatg ctacagaagg
    10321 attattcact gattccctgt tgtctgctgc aggtgtactt catccatctg gttaggatct
    10381 ccactggaac acaggtcaac aaggttgcgc tttgccgggc atgcgattca aacgattgag
    10441 agattgcgcg gtatccggga tggtaagacc tgaaactatt tgaatggcaa aattcgacaa
    10501 atgttgaaat gaagagaaaa gtggcaggta tcttggccct gctgctggtc gcaaccctat
    10561 ttcttgcacc ggctgcagcg ggattcgtga ctccatcgaa accagggctt tccgggaact
    10621 ccctggatta ctttaccccc gtctccccgg gaaatcttcc cggttacgcg gcccagtgga
    10681 gaagcctgga tggtatcaac ccgaacgaac cctccacaat ggccgatctc gcctcggaga
    10741 ttcggaacat gaggtatgga ccgtctcccc agcggccttt cagctggacg ccgtcctgta
    10801 ctatttgaga acaccatcgt ctctctctct tttttggcat acatccgcag gttccggcat
    10861 cgcatcaatt gtatgcatgc gttcctggct tgaagtcctg cctttccact tgtggtgaaa
    10921 gggttttcgc tattgtttct ctccccattg gggtctgttt ccggcatccc cccatatggc
    10981 atgcctgcgg gaatgcgaag gttaaacggg agaggatact gaagatcttc ctcacacaaa
    11041 aggatctctc acgatggcat caacccggcc ggatacgaaa tatttagcca cgcgctcaca
    11101 agaagaggtc ctggcgcagt taggcagctc tcttgcccgg gggctttcgg cggatgaggt
    11161 gtcccggagg ctcgaaaagg aaggatacaa cgagatcact gcaaaccagg tccgttggtg
    11221 ggagatcgcg ctccggcagt tcaggtcggc gttcatctat ctcctcctgg ttgccgcggt
    11281 catcgtcatc ctcatcggcg agtatgtgga cggggtggtc atcctgggtt tcgtcctcgt
    11341 caatgcgctc ctcgggtttt accaggaata caggtctgag caggccctcc aggccctgca
    11401 acagttcatc accccgagaa cccgcgtgct gcgggaatcg cggtgggagg tgcgtgcctc
    11461 gagggatctt gtgcctgggg acattatcag gctcacgaca ggagacgtgg ttccagccga
    11521 cgtgcgggtg ctgacctcgc agaaccttgt cgtgaacgag actgtgctga ccggtgaatc
    11581 tgtgccggta ccaaagctcc ctgacacgct tgacgcggtg cctgtggatg tctacgggag
    11641 ccataatctc tgcttctccg ggaccacggt ggttggcggt gaggcggtcg cggtggtgct
    11701 tgcgaccggt gcgcagaccg cgatgggcac gatagcgaag atgaccgtcg agactacaaa
    11761 ggagagcgag ttctcgaagg ggatcgggag gttcagtcag ttcatcctcc ggatgatcct
    11821 cgtcacgctt gtctttgtgt tcgtcgcaaa cattctcctg aaggggggat ccgtggacct
    11881 ggtcgggctc gtggtcttct ccatcgccct tgtggtcagc gtcatccctg aggcgctccc
    11941 tgtcgtgacc acggtctcgc tttcccgcgg ggcgctccgg cttgcagagc acgacgtggt
    12001 ggtcaaacgg ctctctgcaa tcgaggatat cgggagcatt gatgtcctct gctccgacaa
    12061 gaccgggaca ctcaccgaga acattcttac cgtcaccggg ctctcgcctg gggcagaccc
    12121 ggccctcctc tcctatgccc tgctcacagt cgcggagact gaagagaaga ccgagccgtt
    12181 cgatatcgcc attgtcgacg cggccgcacg gctctctccc ggggagatcc tcgcatcgga
    12241 gcggcttgca gagatcccct tcgacccaaa gacacggtgg aacgcggtac tggtgagcct
    12301 gcccgcaggt cccgtcctcg tcatccgcgg ggcacctgaa gagatcctcc gcctctcccc
    12361 ctctctccct gacgccgggc gcatggcagt tgccgactgg ctttcccgga acgggctggc
    12421 cggggaacgg acaattgcga tagcgaagaa ggcccttcca cctgggaaga cgggaatttc
    12481 tccgggtgat gtccgggacc tcgagttcct cggcgccatg gcgttcaccg acccaatcaa
    12541 gccctcggcg tttcgtgcgg tggagatggc ccgcgaactt ggcgtggccc tcaagatcat
    12601 tacgggggac agccccgaag tcgcaggggc ggtcggcgtg cgcatcggcc tcctcaaaga
    12661 cccggcggcg gtcgttacag gatcggccct tgcggcgatt ccccccgggg agcgggaaca
    12721 ggtgatacag gagcacgcgg tgtttgcccg tatcactccg gagcagaagt tcgagatcat
    12781 cgagaccctg cagcggacct gcactgtcgg gttcctcggg gaggggatca atgatgcgcc
    12841 ggcgctcaag cgtgccgggg tctctctcgt cgttgacagc gctgccgaca tcgcccgcga
    12901 ggcagccgac atcatcctcc tccagaagga tcttgaagtg atcatcgagg gaattcggag
    12961 cgggagggaa gtgtttgcaa acagcgtcaa gtacctgaag gcgacgcttg catccaactt
    13021 cggcaacttc tacgccattg ccattgtctc gctcttcatc ccgttcctcc cgatgctccc
    13081 catccagatc ctgctcgtga acctcctctc cgattttccc atgatctcga tcgccactga
    13141 caccgtcgat gaggcggagt tccgcctgcc agggaggtac gacctgaaag agatcgtgct
    13201 gattgcaacc ctccttggcg tgatcagcac agtcgatgac tttgcgttct tcgcggtctt
    13261 cgtcggggac ggggcacagg tgctccagac cagctggttc atcgggagca tcctgaccga
    13321 acttgtcttc ctcttctcca tcaggacaag acttcccttc taccgggcga gacgcccctc
    13381 gatccatgtc ctggccctga cgactgtcgc cgcggccgca accattcttc tcccctacac
    13441 ctcaataggg caggcactct tctcctttgt gccgccttca cccggggccc tcgcgatcat
    13501 cctcggcctg gtggcagcat tctttgtgac cacagaactg gtgaaggtca gttattaccg
    13561 ctacgctggc ggactccccg cacgctcctg atggtggacg aaaataccgt gtttctcctt
    13621 gggagaggag agttgggagg gagaaagggg agatttcctc ctcctgccca tccgacagga
    13681 agggtccagg caggcaaatt tccggctgcc tgaacagggg atagtataac ctgatgtttt
    13741 ttcataattc cccctgtgca gagatgtgat atgcgtcgca tattctggtg aggtcaataa
    13801 aggcgagccc aatgttcagg atttttttga caaataaata atatcgtaaa ttataaaggg
    13861 gtgtaaaaaa aagacgcaaa tagatatcta ttaattcacc aggtggattt tagaaaatgg
    13921 gcaggcatcc ttttttgaat aaaaactatt ttaggggcga tttcctttga tatcagttct
    13981 ttttgtcgat aatgaacccc acttctgtga attggtaaaa agcacccttg aacaatcagg
    14041 cttattttcg gtcgatactg ctgaatcagc atccgccgca ttgggaaaaa tgaaacatgg
    14101 ttcctatgat gcgatagtat cggactacca gatgccggat atgaatggaa tcgaattttt
    14161 gaaagcactc cgtgcatccg gaaacgaaat cccgttcatc attttcaccg gaaaggggag
    14221 ggaagaggtg gtgatccagg catatgagaa cggggctgat ttctatgtcc agaaaagcgg
    14281 agaccctgag gttcagtatg cagatttagc acagaagatt accagggcag tcggactgca
    14341 ccgggctgag catgaccggc ttgaaagcga ggacaggctg cgacagatca tccagttctt
    14401 acccgatgca acgtttgcca tcgatatgga gggaagagtg attgcctgga acaaggcaat
    14461 cgaggagatg acaggagctc cggcgtcgca gatgatagga aaggggaaat atgaatatgc
    14521 catcccattc tacggagagc gccgcccgat ccttattgac cttgcgatgc agccggatga
    14581 agagaccaca tcgaggtacg catttgtccg caaagaaggg aggttcctgg aggcagaggc
    14641 ttcgaatgca agattgcagg ggaaggatgt cgtattatgg gggaaagcca cacccctgta
    14701 tgacacccgg tgccggctga ctggcgcgat cgagtccatc agggatatca ccgggtttaa
    14761 aaagacagaa gagaccctcc gtgaaagtga agcacgatac cgccggtttg cagagaacgc
    14821 aaaggatttg atttaccgct acacattcac ccccatacgg ggtttcacct atgtctcccc
    14881 ctcctccacg aatatcgtgg gatacacccc cgaagagcat tatgctgacc cggacattgg
    14941 ctggaaactt gtatttgaag atgatcgcca tctccttgag tcacttgcac atgacccggg
    15001 tgtatacaaa aaaccgctcg ttgtaaggtg gaagaagaaa gacggcaccg ttatctggac
    15061 agaacagatc aatcacccca tctacgataa tgaaggaaac attatcgccg tcgaggggat
    15121 agcccgcgat atcaccgatc gaacgcgcag cgaagaggcc atccgggaga agcagatcca
    15181 gctcgccaat gccatggacc tttcgcatct tgtcatgtgg gaatacgacg tggcatcgga
    15241 catgttcacg tttaacgaca atttttactc gctgtatggg acgaccgcat cacaggaagg
    15301 gggataccag atgtcgtcga aggagtatgc ggcgcggttc gtccacccgg atgatgtaca
    15361 tttcgttgca gaagaggtcc agaacgcgtt gaagtcgacg gacccttctt tcagctcgca
    15421 gcttgaacac aggattgtcc gaagagacgg ggaggtccgg tttatcatcg tccgtattgc
    15481 aggagtcgca ggagaatcag gaacccttat aaagacctac ggtgcgaacc aggacatcac
    15541 tgaccgcatt aaagcagaga ccacgttgcg ggagagcgaa gcgcgatatc gcacgtttgt
    15601 cgagaacgct gccgaaggag tggtggtcat ccaggacgga aagatcgtgt acgcgaaccc
    15661 cgcagcgctc cttctcaccc gtgaatccct tgagaacctg ctggagaaac cattcggcca
    15721 atacatacat cccgacgaca gggacatggt ggtcgggaga tacaaacggc gcatgctggg
    15781 cgaagatctc ccctcgatat acgatttccg gctggcaggg accgatgggt acgagacgtg
    15841 ggtgcagata tcagtcgcgg tgatcccctg gaacggccgg ccggcaaccc tcaacttcct
    15901 ctccgacatc accgagcgaa aacagaggga gcagctcctc caaatccagc accgcattgc
    15961 aaccctctgt tcatcgcccg cgcccctcag tgaaatcctg cctgaatgca tgcaccatgt
    16021 cctcgctctc acccggatgg acgccggagg gatcttcctc gttgacgagc atagcggact
    16081 cagcctcgta tattcgacag gactctcgga ggcatttgtg cgcgaagagc agtatcacat
    16141 gcaggactcg ccgcaaaccg agtttgtgat gcagggaaga ccctattttg gggatttctc
    16201 atcgctccag tttccacctt cgccggcacg tgcgagcgaa ggaatcaggg cactggcagt
    16261 agtgccattc gtgcaccagg gcagggtgac aggctgcctg aaccttgcgt cccacacacg
    16321 ggagaccttc ccccaggtga tacaggaggc catctcgact ctcgcgatcc agatgggaag
    16381 cgatatctcc cgaatccagg cgagtgaagc gctcaggaag agcgaggaga agttcaggcg
    16441 gatggtcgag accgcagcag aagggatctg ggagatggac caggactttc acatcaccta
    16501 cgcgaaccag aggatggccg acttgctcgg ctatacacgc gaggagatgg tgggaagaga
    16561 gatctcaacg tttatggaca gggatgaact ccccgactct cttgcccgga aggagcggca
    16621 gcggcaggga atcgccgacc acttcgagcg aaagttgatc cggaaggacg gaatgccact
    16681 ctggatatat tcctctgtaa ctccggtatt cgacgctgat ggaagattcc gcggctcttt
    16741 tgcaatgtac tccgacatca cggatcggaa actgattgag gccgagatcc ggtccctgaa
    16801 ccaggtactc gaacaaaggg tcggggagcg gaccgccgag ttgaccaggt tattgcacga
    16861 acgggaagtc ctgatccgcg aggtccatca ccgggtaaaa aacaacctcc agatcatcat
    16921 ctctctcctg aacctccagg gaaaatatat cacggaccag aaagtccttg ccgccatccg
    16981 ggagagccag aaccggatca agtcgatggc ccttgttcac gagcggatgt accagtccga
    17041 ggaggtctcc cggatcacct tcagagagta tgtcaggttc ctggtctccc atctcttcac
    17101 attctacgat gtcagggcct accaggtctc ctataccatc accatggatc cgttccctat
    17161 cgatatcgat accgccatcc ccctcgggct gattatgaca gaacttgtct cgaacagcct
    17221 caaacatgcc ttccccgcgg gcaggaaggg gacgatctca atcgagggga gaaaggtgga
    17281 tggtcacact ttcaggttcg tcgtgaagga taacggcgtg gggatctctc cagagatcga
    17341 ttggaagaat tcggatacac tcggccttcg cctggtgaaa tccctcgtta cccagctgaa
    17401 cgggaccatc gaattggacc gggcggacgg gacgacattc acgctgcatg tgcgtgcgaa
    17461 agaggcgaag tgattccagg atgatgtatc acgcgtgctt cccgctcctt gggttttcaa
    17521 gagagttgcg aaaggataag gccgcgtgcg gggatggcca aaactgcctc attctctgta
    17581 accactcctg cgacgcaggt ccgcaggcct tttccgaccg ggacaactgc gcgctcatct
    17641 cctgtcgccc aacctattaa tacgcttgat tgtgaactgt gcactgttga cagggtgatg
    17701 cagatggaaa cagaaacgta tgacaaggac aaggtggtct ttgcagccac gatggcctca
    17761 acctttgagc ccgactcaat gacaaacgac aagatcgaag ccgcgaccag gggacatggc
    17821 gccctcgtca tcccggtctt tgcagctgcc aattccatcg ccgaggatct cttctgccct
    17881 atcactggcc cggagatgag cctgatgggg cggatgactg ttgtggatgc atcagtcggg
    17941 gagctccccc tcgatgtggt catccagaag gcggtgcggg ctgcgatgag tgccggggcc
    18001 gctcccgaga acgctgcgct cattgtcgcg tcactcgcgt atttttcggg ttcctgtgca
    18061 cggtcgggtg ttcctctcgg caacaggaaa ctcggcgcca ttgcccggat gcatgccggc
    18121 gcagcccgga caagtgcgat tgcgctggtc accggcaagt ccacccacag aatccagtcg
    18181 ttcccggcat acctcgcgat ctacgaagcc atgatggaca agaagctcac gaaagtggac
    18241 ggctccatgc tccccccgtt catctccggc ggggcaatct atggccactc cgcacttggt
    18301 gaggattaca acatccctga attggcgtac aatgccgcac atgcaggcac gctcgccatg
    18361 atgaagagca tggcgggcac cgggataacc ccactcgcgc tctggcctgc ccttatcggc
    18421 gcggcggtca cgatggagat agtgcacccc gatgcactcc tgggcgagga gtttgggaag
    18481 tttggcaggg tcgattcatc gcatctcgcg ggaaaaggag cacgcgatgc cgcgaaactg
    18541 ccagaaaaga ttcacatccg gggaactcac gaggagtacg acacggccaa aatcatcggc
    18601 gagttcgggc tcatcctcaa ggatattggc ggcccttcgg tgatcggatc gatggccctc
    18661 tcagagattt tcgccgcctt tgaagaagcc ccgatgatcg gggcagggtt ctcggggggt
    18721 cccgtcaacc cgccgctcgg ccacctcgaa ggcgactgcg tgccggcgct ccgcctcctc
    18781 atgaaacacc atggcgacat cttcgcggtc gccgaagtga tccgagatta caagatgaac
    18841 tcattcatca
//
LOCUS       DAOQYR010000039        18570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_3371_length_18570_cov_8.207777, whole genome shotgun sequence.
ACCESSION   DAOQYR010000039 DAOQYR010000000
VERSION     DAOQYR010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 18570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18570
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3371_length_18570_cov_8.207777"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..731)
                     /locus_tag="PKM87_06145"
     CDS             complement(54..731)
                     /locus_tag="PKM87_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-related McrA-glycine thioamidation protein"
                     /protein_id="HNQ29683.1"
                     /translation="MPQEIAVFLGPSCERAVAETILPARFFHPPAARGDLTDAAEEGA
                     RIITLIDGVFFQESAVGHREILAALKSGIRVIGASSMGALRAAELDTLGMEGVGLIYR
                     LYRDGVLISDDEVALVYDPEDYTPLSEPLINIRCTIRKARDEGILTNDDAAALLSAAR
                     SLYFPERTYPHICEQAESAVFAPVRKSFLEFARLHAVDQKREDALEALRRTKELLGKQ
                     LGEQLGL"
     gene            complement(691..1905)
                     /locus_tag="PKM87_06150"
     CDS             complement(691..1905)
                     /locus_tag="PKM87_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-related McrA-glycine thioamidation protein"
                     /protein_id="HNQ29684.1"
                     /translation="MILSPCKKAYKNETQRTTSPEETLARVEKALPAAGITRVADITS
                     LDRVGIPVFSSIRPTAGRGAISVYNGKGATPTEARVSAIMEGIERYSAEVPEGAELRI
                     DRYEEVRRDATVLDPLLLVLPRNADPMMPIPWVEGYDLLQEEEVMVPASAVFHPLPAK
                     YPQLFRTSTNGLASGNTLEEAVFHALMEVIERDAWSLAEASRNTGPRVTRINGDLPGD
                     LLSRFESAEVEVRVKDITSDLCIPTFAAVSDDLRLRDPMLLTIGMGTHTSAGIALLRA
                     LTEVAQSRLTQIHGAREDTPTADMRKRMGYERARRMNRYWFDSPEEEQFSEVPSFDSD
                     DFLTDIRMTLDRLEGAGMDRVIVVDLTRPEINVPVVRVVVPGLEVYAMDQERMGARCH
                     AARDRRIPGPKL"
     gene            complement(2000..3361)
                     /locus_tag="PKM87_06155"
     CDS             complement(2000..3361)
                     /locus_tag="PKM87_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNQ29685.1"
                     /translation="MHWLFYVLLAVAAYAIVAGYIYTRKAAYERERADRIAFPEMAEG
                     RPERSFWDAIVFYGPIIAIKTERVAFFDRFTRFSTLLRLYASFGVVMVVIISAGIALI
                     LFVSVQFTLLLRPEPTGIYAPQNILLIPGLNEYVPSTLAVWFAFVLTIAIHEFGHGIL
                     CRVEKIGVRSMGALIAVIPIGFFVEPDEQELEKSRGLPKVRMFGAGITNNIVIGAICF
                     VALFLLAGMAVPPSGPVLAGVYQNYSAWEAGVPTPSLITGINGVPVGTREDVSALLNG
                     TRPGDALALTVEYQGQQSSYALTLSEWPKELGERDSGFMGVHYYDGAMIMDQVTQGIS
                     VPGFFRFLTIPFDPSMGGQALRMLAFETPESQFYQEPFPGFWGAVHLLFWCAWININV
                     GIFNAIPMVPLDGGYILKEGVERLFERRGLSKYALPVVSFISSLMLVMLFSIIFLPYF
                     LHA"
     gene            complement(3421..4071)
                     /gene="rnhB"
                     /locus_tag="PKM87_06160"
     CDS             complement(3421..4071)
                     /gene="rnhB"
                     /locus_tag="PKM87_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNQ29686.1"
                     /translation="MLCGVDEAGKGAVLGPLVVAGVACRRKQDLAKLGVKDSKALSPA
                     RRETLAAELKASFPVEVLVISPGEIDSRGMTMNALMAKSHARVIDALHPAVAVVDACD
                     VVAARYGAMVSSHLDRPCRVVSRHHADQHHPAVSAASIVAKVERDHAIEHLKREFGEI
                     GSGYPADPFTVRFLEEYVSTNQGPPPIARRSWETVRVLLARHEQASLFDFPGGDTE"
     gene            4130..4798
                     /locus_tag="PKM87_06165"
     CDS             4130..4798
                     /locus_tag="PKM87_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNQ29687.1"
                     /translation="MYIIIVGLGGIGRNLTQLAVEHGNNVVIIDRDEQRCSEILEHHD
                     VLAITGNATDKSILEDAGIDRADALVATTSDDSVNLMTCWLAKRFKVPNVVSIVNQKE
                     HSDLFAEVGVKISENPDELVAQRLYYWAENPQMQQLASIPGGTIFEVVAEKGAPIVDH
                     EIRELKVKDFVFIAIRRAGGELIIPSGTVKIRPGDTITVFTKKEAEDDCLAILNRQLR
                     RSPE"
     gene            complement(4787..5983)
                     /locus_tag="PKM87_06170"
     CDS             complement(4787..5983)
                     /locus_tag="PKM87_06170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ29688.1"
                     /translation="MKSRYDVVVVGGGPGGAIAARTAAEKGLSVFLAEKRPAVGAPVR
                     CAEGIGKEALAEFIDPDPRWISAELKGAEIVAPDGTSMKLASEQAGSKVGYILDRKVF
                     DRELVWRAAEAGADVAVKTRAVAPVLDGGAVKGAVFEYCGERQRVEADVVIAADGVES
                     KFSRWCGIDTTVPVREIMTSAQYLMTDIDIDPYTTVFLLGNEVAPEGYLWIFPKGERT
                     ANVGIGISGKKSGAGHRARDYLDRFVSSRFPGGKTIEHIVGGVSVCRPLPCTVADGLM
                     IVGDAARVVDPLTGGGIYNAMFTGKLAAGTAAAAISRGDCAKAALMPYDKAWRDSPMG
                     KAIARNWQIKEYLIKLPDGKLNAIIQSAASLNLKEFSTLSLIKELMKRNPKLVVELAA
                     LKASLG"
     gene            complement(5967..6149)
                     /locus_tag="PKM87_06175"
     CDS             complement(5967..6149)
                     /locus_tag="PKM87_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ29689.1"
                     /translation="MLQVHREICGYCGCCVSVCPEGALELIDAYLEVGDTCTSCGICA
                     KVCPLGALEVKDEVQV"
     gene            complement(6234..7385)
                     /locus_tag="PKM87_06180"
     CDS             complement(6234..7385)
                     /locus_tag="PKM87_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HNQ29690.1"
                     /translation="MNSGTSSDCRHVTCDLHLHSRFSIASSPRMVPHAMVEAARRKGL
                     VAIGSGDALHPAWRREWEVFLSGRDPEEVIVVPSSEVEDRNRVHHLVLMDDFSGFSDL
                     QETFALSGKDIATTGRPRLRHSGEEIACAVHDAGGLVGPAHAFTPWTSMYASHDHVGE
                     CYGSEPIDFLELGLSADNSYGAAIPDLYGVPFISSSDAHGPDPWRMGREYVVLEVKEA
                     RPAGVLEAVSGGRVVQNVGLFPEEGKYNRTACSRCFRQFTSDEAAAHAWKCPGDAGRI
                     KKGVFDRARELSTGESRPRPPYQHMIPLPEILQRVLACSSPQARHVGNLYSRFVERFG
                     DEIRVLTEVPVEDLAAVHEGTASAIDALRCGRVRLSPGGGGRYGTFSFL"
     gene            complement(7363..8115)
                     /locus_tag="PKM87_06185"
     CDS             complement(7363..8115)
                     /locus_tag="PKM87_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HNQ29691.1"
                     /translation="MDEITVRYLGIEPGEVSAPVLIEGLPGIGHVGKLVAEHLIHELG
                     ARKIAEIESIFFPPQVIIGENGTVRLANNEIYFYDGEDRRILFLVGDFQSTSAEGHYL
                     LADTFLSIAEELGVSRTYTLGGYGVGHLVDVPRVLGAVNDGMLRDEIEAAGAVISPDE
                     PGGGIIGAAGLLLGLGRARGIEGICLMGETSGYLVDPKSASRVLDVLCTLLDLRVDAT
                     TLQERGMEMEHMIQKLVDTERAAESDELRYIV"
     gene            complement(8103..8264)
                     /locus_tag="PKM87_06190"
     CDS             complement(8103..8264)
                     /locus_tag="PKM87_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-protein complex protein Nop10"
                     /protein_id="HNQ29692.1"
                     /translation="MSGRLRRCPLDRTYTLQERCPACGEPTVTAHPPRFSPEDRYGHY
                     RRVMKGWTR"
     gene            complement(8261..9046)
                     /locus_tag="PKM87_06195"
     CDS             complement(8261..9046)
                     /locus_tag="PKM87_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     alpha"
                     /protein_id="HNQ29693.1"
                     /translation="MSEREWPEQAELVVCTVKDVKDFAAFVSLDEYPGRVGLIPISEV
                     ATGWIKYIRDHIREGQKVVCKVLHVDTQRGHIDLSLKDVNEHQRREKIREWKNESKAR
                     KWIGFAAQAAGVKAEEIEASIYSHYGDLYSVFEDIVSGEHSLDSLGISPGAASALQQV
                     ARENVKMPRVTVGGNIILTSTKPDGVNIIRRALRSAEPKIPDVDIELSYLGAPAYRIK
                     VTARDYKTAEKAIEKAAAAAIGVVERAGGEGKFIKKPKSGKTA"
     gene            complement(9055..9243)
                     /locus_tag="PKM87_06200"
     CDS             complement(9055..9243)
                     /locus_tag="PKM87_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27e"
                     /protein_id="HNQ29694.1"
                     /translation="MVRQLRENRSKFFRVKCADCENEQVVFEKASTTVECVVCGKVLA
                     EPSGGKAQLKAEVLATLE"
     gene            complement(9249..9527)
                     /locus_tag="PKM87_06205"
     CDS             complement(9249..9527)
                     /locus_tag="PKM87_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L44e"
                     /protein_id="HNQ29695.1"
                     /translation="MKMPVKFKTYCPFCRSHQVHEVEKVKKSRTSGLHWIDRQKARRS
                     TVGSMGKFSKVPGGDKPTKKINVRYRCSTCKKAHLRGGFRAGKFELTE"
     gene            complement(9565..10182)
                     /locus_tag="PKM87_06210"
     CDS             complement(9565..10182)
                     /locus_tag="PKM87_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29696.1"
                     /translation="MHLDELRILLLSERESGRLVQIQKDLFSSVGAGLETHYSSLYAC
                     EDPFSDEARVLIERIAAIRETLLDLYRIRAEKILSLARSQEDGQYVDREELKKLLPEE
                     KDMFDQVVLALEQARCRLVDGSVPRAPSHLGSEREADRCASVLAAAAERPAFVVSRVL
                     QDMEPFMGVDGHIYHLQREDLVTIPERNAEVLCERNIVLNINPGK"
     gene            complement(10169..11335)
                     /gene="priS"
                     /locus_tag="PKM87_06215"
     CDS             complement(10169..11335)
                     /gene="priS"
                     /locus_tag="PKM87_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase catalytic subunit PriS"
                     /protein_id="HNQ29697.1"
                     /translation="MKAATLEFLRQRFSSYYAKEPLLSPSSLPQREWAFVFFDPDFPD
                     IRMRRHLGFETRDEAFSYIRSMAPAHIYYSTAYYARPGAGTMQEKEWLGADLIFDLDA
                     DHIMRGPYDRMLSRVKEETKKLLDMLVLELGFSPREIDLVFSGGRGYHVHVHDLSVRN
                     WGSSERRELIDYVCGIGLDPGRVLAHRTHSPRGWHHRYIAALAEYLLWLESLGEKERV
                     AAIAGLEGVGKTTAEEFSRRLGEVLSRITTDPASLNLKDAVIAKVIRGLSVQKEGQFA
                     AMLRDRAALADEPVTTDTKRLIRLPSSLHGGSGLRVTPLAPKDLDDFDPLVDAVVFGE
                     REVKVDLAFPLSMPILGTSFRLEKGISAVPEALAVFLCCRGAAEIAGGASSAPG"
     gene            complement(11332..12906)
                     /locus_tag="PKM87_06220"
     CDS             complement(11332..12906)
                     /locus_tag="PKM87_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HNQ29698.1"
                     /translation="MARPDISREILEAIFSRDAAPGDITRIKIEVCKKYGAAAVPKNS
                     ALLAAALPGERERLRALLLVKPTRTLSGVAPVAVMTSPSPCPHGKCLPCPGGPDHPFQ
                     SPQSYTGEEPAALRAREHGYDPYSQVRARLEQFELLGHHVDKVELIVMGGTMTARDPE
                     YQEWFVASCIRAMNEYRGHPAGAATDTASVFLQNEMSPVRCVATTFETRPDWCREEHV
                     ERMLALGVTKVELGVQHLDDRILAYNRRGCTVEDTIAANRLLRDAGIKVGFHVMPNLP
                     GSSIERDRWMFQELFENPDFRPDFLKIYPTLVTPGSEIESLWMRGRYAPYDEDDLIGL
                     VAYGKSLLPEYVRLQRIQRDIPAKLIVAGSRHSNFRQLCQQRLAAMGGRCRCIRCREA
                     GRSAGGGAVEYRTFSYACTGGKEHFISAVSGDALIGFARLRCGGQRWREELVDAALLR
                     ELHVYGSIVPIGSSPAHDQRQHRRFGRVLLSTAEETARSAGYREIAVMSGIGVRPYYR
                     RHGYERRGPYMHKRLS"
     gene            complement(12913..13179)
                     /locus_tag="PKM87_06225"
     CDS             complement(12913..13179)
                     /locus_tag="PKM87_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0058 family protein"
                     /protein_id="HNQ29699.1"
                     /translation="MQKEELLHLHMLMIHIKKYYETITSHEIPTKRYNSLHISPVHIH
                     KNKKCHKDAILALGDEIVTHLQSTQMVPVGYATETASEKVAAEH"
     gene            13233..14117
                     /locus_tag="PKM87_06230"
     CDS             13233..14117
                     /locus_tag="PKM87_06230"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNQ29700.1"
                     /translation="MNFISDIRHSTAALLGLAIGDAMGAPLEGVVRPAAPVRRMYAGG
                     RIARHSGACTDDTYQALALAESLAACRGFSPPDFMERLVRDYLFFGEWYGPTSGAVFS
                     LVIGGTPLYSAARLVHARRGWSRSNGSVMRGPPIGVFYFGPEVEAASMACSALTHRDP
                     VAGACSAFVNRMVSDMCRGMPKERAYARALQRCRDAEVAGMLGRWREFPPSPGLDALE
                     ATHAAVSVFMETFSFEEAVPRAVSMGGDSDTVGAIAGALAGAAYGMHSIPEEWIRGLS
                     DSGRVLRAAHALWLASRG"
     gene            complement(14124..14570)
                     /locus_tag="PKM87_06235"
     CDS             complement(14124..14570)
                     /locus_tag="PKM87_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0179 family protein"
                     /protein_id="HNQ29701.1"
                     /translation="MAEVKPKVTLIGTTLARVDLEFIYEGECAECEGCTVRKACHNLK
                     PGRRYRVVGVRKTHHACPLHLEGATAVEVVESSIAALVSADMAITNTRILFEPSCSRD
                     KCEHFRLCNPEGVIPGEKYVVVRVLGNAPPGCVKGRSLQLVELRAV"
     gene            complement(14577..15656)
                     /locus_tag="PKM87_06240"
     CDS             complement(14577..15656)
                     /locus_tag="PKM87_06240"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-1-phosphate
                     dehydrogenase"
                     /protein_id="HNQ29702.1"
                     /translation="MSADPIKVLKTKVFDKSRWMQLPRDVVIGHDVLPQIPSVCADLC
                     LKGRVVVFSGTRTRECAGDHVASLLSERYEVSTFSVREISMDTIHEAEEFSRGAGFLA
                     GVGGGRVIDCAKIVSYNLDRQFISVPTAASHDGIASARASVPMESGSVSLEAHPPIAI
                     VADTGLIAAAPHRLLASGCADVISNSTAVLDWELAHRLKHEEVSEYAVALSRMTAELL
                     MKNAKGIKPHQEESAWMVTKALVSSGVAMSIAGSSRPGSGGEHKFGHALERLAPGAAL
                     HGEACGIGTIIAMYLHGGDWRAIREALRSIGAPITPADLGIDDGVAVEALLAAKDIRP
                     ERFTILDMGLTRESATELVRMLYRE"
     gene            15669..16517
                     /locus_tag="PKM87_06245"
     CDS             15669..16517
                     /locus_tag="PKM87_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF63 family protein"
                     /protein_id="HNQ29703.1"
                     /translation="MIWDFIQKYYIDPIRYGQPYNVVDTTTYALVLILAIYIIYRWLS
                     RSEIIRIDRKFILCTLPFVVMGGLLRVVQDTGMIESDWQYLLITPLIYFVMFAYTAGA
                     LVISVTLQEHGALKDYHRGYLAAGIAGAAATGLVLLWFGAENGIIALDVLAIILAMAV
                     ATTAAVYAAMRYLLKWEYVADPLYLSLLFGQLLDASATSYGIDLHPLHYVEVHVVGSH
                     LIEWTGTAFSMFGLKLLVLFPGIYILERFRHEGPGALWHLILLAMITVGLAPGIRDMM
                     RMVLYV"
     gene            16510..17070
                     /locus_tag="PKM87_06250"
     CDS             16510..17070
                     /locus_tag="PKM87_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNQ29704.1"
                     /translation="MSDLSRNAGIVVILLAVSVAFGAWATMQDPAVGEQLISLLKESI
                     MGEALDSSGAMMAVMLFFNNLQACVLMFIGGASLGLLTVFIISTNGFVIGSVLEMVRE
                     EHSALYVAAAIIPHGIFEIPAFVVSGALGLMLAKALWKEWEGSEDAASRALAYARTFL
                     LAVVPLVAVAACVEAFITPEILRLAI"
     gene            17081..18517
                     /gene="proS"
                     /locus_tag="PKM87_06255"
     CDS             17081..18517
                     /gene="proS"
                     /locus_tag="PKM87_06255"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNQ29705.1"
                     /translation="MAEEDQGALPPKGDFSAWYNELLWRAEVMDVRYPVKGLYVWYPF
                     GFSIRRNVYDMLRRLLDRDHQEALFPLLIPENELAKEAEHIKGFEDEVYWVTHGGLTP
                     LDVKLALRPTSECAIYPMYALWVRSHADLPLKIYQIVNTFRYETKQTRPMIRAREITS
                     FMESHTVHATWDEAETQVEQEIALTLEFYDSLCIPLIISKRPDWDKFPGADYTVAVDT
                     VMPNGRTLQIGTVHHLGTHFSKTFGISFEDEKGAQKPANQTCYGISERSVAALIGMHG
                     DDRGLVLPPGPAPVQVVIIPITIGKRKDEVGAAAAGIEKELSAAGFRVKIDSRDIRPG
                     AKYYYWEMRGVPLRLEVGPRDIDAGQVTAVTRLGEKSQIARDALVEGVTGRLASFAAT
                     LKERAEAHVRTHMRVSRSMDEVAAAVEDGVTLVPWCGSRVCADLIEEKTRAAVLGTEV
                     RSAYLVDASGPCVACGKQGTTALVGRSY"
BASE COUNT         3263 a         5927 c         5833 g         3547 t
ORIGIN      
        1 cggagggggt tcccccctcc ggcgcctccc ccgcacaccc cccgcagttg gggctaaagc
       61 cccaactgct cccccaactg cttccctagc aactctttcg tcctccgcaa tgcctccagc
      121 gcgtcctccc tcttctgatc gaccgcgtgg agacgggcaa actcaaggaa cgacttcctg
      181 accggagcaa aaaccgcact ctctgcctgc tcgcagatgt ggggataggt ccgctccggg
      241 aagtagagcg aacgggctgc agagaggagc gctgcagcat cgtcgttggt gaggatgccc
      301 tcatccctgg ccttcctgat ggtgcagcgg atattgatga ggggctcgga gaggggggta
      361 tagtcttcgg ggtcatagac gagggcgacc tcgtcgtcgg agatgaggac cccgtcccgg
      421 tacaggcggt agatgagtcc cactccctcc atccccagag tgtcgagttc cgcggcgcgg
      481 agcgccccca tgcttgaggc accgatcacc ctgataccgg atttgagcgc cgccaggatc
      541 tcccggtggc cgacggcgct ctcctggaaa aagaccccgt cgataagggt gatgatgcgg
      601 gcgccttcct ccgcggcgtc ggtcagatcg ccccgtgcgg cgggcgggtg gaagaaccgg
      661 gccgggagga ttgtctccgc gactgcccgt tcacagcttg ggcccaggaa tacggcgatc
      721 tcttgcggca tggcaccgtg cccccatacg ttcctggtcc atggcataga cctcgaggcc
      781 cggcacgacc acccttacca ccgggacatt gatctccggg cgggtgaggt ctaccacgat
      841 cacccggtcc atcccggcgc cctcgaggcg atcaagggtc atcctgatgt cggtcaggaa
      901 gtcatcgctg tcaaaagagg ggacctcgga aaattgctct tcttcgggcg agtcgaacca
      961 gtaacggttc atcctcctcg cccgctcata tcccattctc ttgcgcatgt ccgctgtcgg
     1021 ggtgtcctcc cgggcgccgt ggatctgggt caggcggctc tgggcgacct cggtcagcgc
     1081 acgcaggagc gcgatgcctg cgctcgtgtg ggtccccatc ccgatggtga ggagcatcgg
     1141 gtcccgcagc cggaggtcgt ccgagaccgc ggcgaacgtg gggatgcaaa ggtcgctcgt
     1201 gatatccttg acccgcacct ccacttccgc cgactcgaaa cgggagagga ggtctcccgg
     1261 caggtccccg ttgatacggg tgacccgggg ccccgtgttc ctgctcgcct ctgcaagcga
     1321 ccacgcgtcc cgctcaatta cctccatgag ggcatggaag actgcctcct cgagggtgtt
     1381 tccggacgca agcccgtttg tcgaggtccg gaagagctga gggtacttgg ctgggagggg
     1441 gtggaagacg gcgcttgccg ggaccatgac ctcctcttcc tgcaggaggt cgtacccctc
     1501 cacccaggga atcggcatca tggggtccgc atttcgcggg agcacgagaa gcagggggtc
     1561 gaggacagta gcgtcccttc gaacctcctc gtaccggtcg atccggagtt ctgcgccctc
     1621 gggaacctcc gccgagtacc gctcgatccc ctccattatg gccgagaccc ttgcctcggt
     1681 cggggtggcg cccttcccgt tgtagactga gatcgcaccc ctccctgcag tcgggcggat
     1741 gctcgagaag acagggatcc ctacccggtc gaggctcgtg atgtccgcga ccctcgtgat
     1801 ccctgcagcc gggagcgcct tctccacccg tgcgagggtc tcctccggag aggtggtccg
     1861 ctgcgtctcg ttcttgtagg ctttcttgca gggcgagagg atcatggagg gcgtccgttc
     1921 cggttgttgc aggttctagt tttgggagaa actcacatat aggttccttg ggaccgccct
     1981 ctgcatgggg cccagggcgt tacgcgtgga ggaagtaggg gaggaatatg atggagaaga
     2041 gcatcaccag catcagggag gaaatgaagg atactaccgg cagcgcgtat ttcgaaagcc
     2101 ccctccgttc aaaaagtctc tccactccct ccttgaggat atagccgccg tcgaggggga
     2161 ccatcgggat ggcgttgaag atgccgacgt tgatgttgat ccatgcgcac cagaagagga
     2221 ggtgcaccgc tccccagaac cccgggaacg gctcctggta gaactgcgat tcgggggtct
     2281 cgaaggccag catgcggagc gcctggcctc ccatggaagg gtcgaagggg atcgtcagga
     2341 accggaagaa ccctgggacc gagatgccct gcgtcacctg gtccataatc atggcgccgt
     2401 cgtagtaatg cacccccata aagcctgagt ccctctcccc gagctctttt ggccattccg
     2461 agagggtgag ggcgtaactc gactgctgtc cctggtattc cactgtcagg gcgagggcat
     2521 cgcccggccg ggtgccgttc aggagagccg agacgtcctc gcgggtccct accgggaccc
     2581 cgttgatgcc ggtgatgagg gagggagtgg gaactcccgc ttcccacgcg gaatagttct
     2641 ggtagactcc tgcgagcacc ggccccgagg ggggcaccgc cattcccgcc aggaggaaaa
     2701 gcgccacgaa gcaaatcgcc ccgatcacga tgttgttcgt gattcccgcc ccgaacatgc
     2761 ggactttcgg cagcccccgg cttttctcga gttcctgctc gtcgggctcg acgaagaacc
     2821 cgatcgggat gaccgcgatc agcgcaccca tgctccgcac cccgatcttc tccacccggc
     2881 agaggatccc gtgcccgaac tcgtggatcg caatggtcag gacaaacgcg aaccatactg
     2941 ccagggttga ggggacgtac tcgttgagcc ctggtatcaa caggatattc tgtggtgcgt
     3001 agatgccggt gggctcgggg cggaggagga gggtgaactg gaccgagaca aagaggatga
     3061 gtgcaatccc ggcagagatg atcaccacca tgaccacccc gaacgaggcg tagagccgga
     3121 gcagcgtgga gaaccgggtg aaacggtcga agaacgccac ccgctcggtc tttatggcaa
     3181 tgatcgggcc gtagaagacg attgcatccc agaaggaccg ttcaggcctc ccttctgcca
     3241 tctccgggaa ggcgatccgg tcggctctct cacgttcgta ggcggccttc ctggtataaa
     3301 tatagccggc tacaatggca tacgccgcta ccgccaggag cacgtaaaag agccagtgca
     3361 ttcctgtata ttacgcggga aatctacata aacctgtgcg aaatgacagg aatcacgggc
     3421 ttattccgtg tctccgcccg gaaaatcaaa aagacttgcc tgttcgtgcc tggcgaggag
     3481 gaccctgact gtctcccagc tcctccgcgc aatgggcggg ggtccctggt tcgtgctgac
     3541 atactcttcg aggaaccgga cggtgaaggg gtccgcgggg tagccgcttc cgatctcccc
     3601 gaactctcgt ttgaggtgct cgattgcgtg gtcccgctct acctttgcca cgatgcttgc
     3661 cgcagagacc gcgggatggt gctggtcggc gtggtgccgg gacaccaccc tgcaggggcg
     3721 gtccaggtga gacgagacca tggccccgta ccgggcggcc accacgtcgc aggcgtccac
     3781 gacggcgacc gcggggtgga gtgcgtcaat cacccgtgca tgggattttg ccatgagggc
     3841 gttcatggtc atgccccgcg agtcgatctc cccaggggag atcaccagga cctccaccgg
     3901 gaacgaggcc ttcaattccg cggcaagcgt ttcacgacgg gcaggcgaca gggcttttga
     3961 gtccttcacc ccgagtttcg caaggtcctg cttcctccgg caggcaacac ccgccaccac
     4021 gaggggtccg agcaccgcac cttttcctgc ctcgtccacc ccgcagagga tcatcggtac
     4081 agttgcggcc cgcaaaatat ttgaaggccg ctggttgaac ccttaacgca tgtatatcat
     4141 catcgtgggg ctcggcggga tagggcggaa cctcacccag cttgcggttg agcatgggaa
     4201 caacgtggtg atcatcgacc gcgacgagca gcgctgcagc gagatactgg agcaccacga
     4261 tgtgcttgcg atcaccggga acgcaaccga caagtccatc ctcgaggacg ccgggataga
     4321 ccgggcggac gcgctcgtgg ccacgacgag cgacgactcg gtcaacctga tgacctgctg
     4381 gctcgccaag cgtttcaagg tcccaaacgt cgtctccatc gtcaaccaga aagagcactc
     4441 ggacctcttc gcagaggtgg gggtgaagat ctccgagaac cccgacgagc tggtggcaca
     4501 gcggctctat tactgggctg agaaccccca gatgcagcag ctcgcctcga ttccgggcgg
     4561 cacgatcttc gaggtggtcg cggagaaggg tgcgcccatc gtcgaccatg agatccgtga
     4621 gctgaaggtc aaggacttcg tcttcatcgc catcaggcgg gcgggaggcg agctgatcat
     4681 cccgagcggc acggtgaaga tacggccggg ggatacgatc acggtgttca caaaaaaaga
     4741 ggccgaggac gactgcctcg ccatcctcaa ccggcagctg cgcaggtcac ccgagtgacg
     4801 ccttgagcgc tgccagttcc accacgagct tggggttcct cttcatcagc tcttttataa
     4861 gactcagggt gctgaattcc ttcaggttga gcgatgcggc cgactggatg atggcgttca
     4921 gtttcccgtc cgggagcttg attaagtatt ccttgatctg ccagttccgg gcgatcgcct
     4981 ttcccatcgg ggaatcccgc cacgccttgt cgtagggcat aagtgcagcc ttcgcgcagt
     5041 cgcccctgga gatggccgcg gcggcagtcc cggcggccag ttttccggtg aacatggcgt
     5101 tgtagatgcc tccccctgtg agggggtcca ccacccgtgc ggcgtctccg acgatcatga
     5161 gcccgtctgc caccgtgcag ggaagcggcc tgcagaccga gaccccgccg acgatgtgtt
     5221 cgatggtctt tccgcccggg aaccgggagg agacgaacct gtccaggtag tcgcgtgccc
     5281 ggtgccctgc gccgctcttc ttcccgctga tcccgatccc gacgttcgcg gtgcgctcgc
     5341 ctttcgggaa tatccagagg tacccttccg gcgccacctc gtttccgaga aggaagaccg
     5401 tggtataggg gtcgatgtcg atgtcggtca tcaggtactg ggccgaggtc atgatctctc
     5461 tgacagggac cgtggtgtcg atgccgcacc agcgcgagaa cttggactcc acgccatcag
     5521 cggcgatcac gacatctgct tcgacccgct gccgctctcc gcaatactcg aacaccgccc
     5581 ccttcaccgc tcccccgtcg agaacaggtg cgactgccct cgtctttacc gcgacgtcag
     5641 ccccggcctc tgccgcccgc cagaccagct cccggtcgaa taccttcctg tccaggatgt
     5701 acccgacctt gctcccggct tgctcggatg caagcttcat cgaggtcccg tcgggcgcaa
     5761 cgatctcggc ccctttcagt tccgcggaga tccatctcgg gtcagggtcg atgaactcgg
     5821 ccagcgcctc ctttccaatc ccctctgcac accggaccgg cgcaccgacg gcagggcgct
     5881 tctcggcgag gaatacggag agtcctttct cggccgcggt gcgggcagcg atggctcctc
     5941 ctggaccccc gcccaccacg accacgtcat acctggactt catccttcac ctccagcgca
     6001 cccagggggc agacctttgc acaaatcccg caactggtgc aggtgtcgcc cacctcgagg
     6061 taggcatcga tcagctccag ggcaccctcg ggacagaccg agacacaaca cccgcagtac
     6121 ccgcatatct cacggtgaac ctgcaacatc cgggtacaat cggcgcggag caccctaaaa
     6181 cttttgctca tgcctatgga gaatccccgg attctggaaa accggaagga ttgtcagagg
     6241 aatgagaatg tgccatacct tcccccgccg cccggagaga ggcggaccct accgcaccgg
     6301 agcgcgtcga tggccgacgc ggtcccctcg tgcaccgcgg cgaggtcctc aaccgggact
     6361 tcggtcagca cccggatctc gtctccgaac cgttcgacga acctgctgta caggtttccg
     6421 acgtgtcgtg cctgcggcga ggagcaggcg agcacccgct gcaggatctc cggcagcggg
     6481 atcatgtgct ggtagggagg acgtggccgg gactctccgg tggagagttc cctcgccctg
     6541 tcaaagaccc ctttcttgat ccgtcccgca tcaccagggc acttccaggc atgcgctgcc
     6601 gcctcgtcag aagtgaactg acggaaacag cgggagcagg cggtgcggtt gtacttcccc
     6661 tcctcgggaa agaggccgac gttctgcacg acacgcccac cggagacggc ctcgagaacc
     6721 cccgcaggtc tcgcctcctt cacttctagt accacgtatt ccctccccat cctccaggga
     6781 tctgggccgt gggcatcgga actggagatg aacgggacgc cgtacaggtc ggggatggcc
     6841 gcgccgtagg agttgtccgc ggagagcccg agctcgagga agtcgattgg ctcgctcccg
     6901 tagcactccc cgacatgatc gtggctcgcg tacattgatg tccagggagt gaacgcgtgg
     6961 gcggggccga cgaggccgcc tgcgtcgtgc acggcacatg cgatctcctc gccagagtgg
     7021 cggaggcgcg gccttccggt cgttgcgatg tcctttcccg acagcgcgaa cgtctcttgc
     7081 aggtcggaaa aaccggagaa gtcgtccatc aggacgaggt ggtggacgcg gttccggtcc
     7141 tcgacctctg acgagggaac gacgatgact tcctccggat cacggccgga taagaatacc
     7201 tcccattcgc ggcgccatgc cgggtggagc gcatcgccgc tcccgattgc gacgaggccc
     7261 tttcttcttg cggcctcgac catggcatgc gggaccatgc gtggagacga ggcgatggag
     7321 aaacgggagt ggaggtgcag gtcgcaggtc acgtgcctgc agtcagacga tgtaccggag
     7381 ttcatcgctc tcggcggcgc gctcggtgtc cacgagcttc tggatcatgt gctccatctc
     7441 catccccctc tcctggaggg tcgtggcgtc gacccgaagg tcgaggaggg tgcagagcac
     7501 gtcgaggacc cgtgatgcgc tctttgggtc gaccaggtag cccgaggttt cgcccatcag
     7561 gcagatcccc tcgatacccc gcgcccgccc gaggccgagc agcagccccg cggccccgat
     7621 gatcccgccg cccggctcat ccgggctgat gaccgcacct gcggcctcga tctcgtcgcg
     7681 aagcatgcca tcgttgaccg ccccgagcac ccgcgggacg tccaccaggt gcccgacgcc
     7741 gtagccgcca agggtgtagg tgcgggagac cccaagctcc tccgcgatcg agaggaaggt
     7801 gtccgcaaga aggtagtgtc cctctgcaga cgtgctctgg aagtcgccga ccaaaaagag
     7861 gatccgccgg tcctcgccgt cataaaaata gatctcgttg ttcgcgagcc ggaccgttcc
     7921 gttctccccg atgatcacct gcggcgggaa gaagatcgac tcgatctccg cgatcttcct
     7981 tgcgccaagt tcgtggatca agtgctcggc cacgagtttt ccgacgtggc cgatgccggg
     8041 gagcccctca atgaggacgg gggcggagac ctcaccaggc tcgattccga ggtagcggac
     8101 agttatctcg tccatccctt catcaccctc cggtaatggc cgtagcggtc ttccggtgag
     8161 aaacgtgggg ggtgggcggt cacggtgggc tcaccgcatg cggggcaccg ctcctgcagg
     8221 gtataggtcc ggtcaagggg acaccgcctc agccgtccgc tcatgcagtc ttcccggact
     8281 tgggtttctt gatgaatttt ccctcgcccc cggcgcgctc cacgacgccg atggccgcgg
     8341 ctgcagcctt ctcgattgcc ttctccgccg tcttgtaatc gcgggcagtg accttgatgc
     8401 ggtaggccgg ggccccaagg taagagagct cgatgtccac gtcagggatc tttggctcgg
     8461 cgctccggag cgccctcctg atgatgttca ccccgtcggg tttcgtggag gtgagaatga
     8521 tatttcctcc cacggtgacc cggggcatct tcacgttctc gcgtgcgacc tgctggaggg
     8581 ccgaggccgc accgggcgag atgccgagcg agtcaaggga gtgctcgccg ctgacgatat
     8641 cctcgaacac cgagtagagg tcgccgtaat gggagtagat cgacgcctcg atctcctcgg
     8701 cctttactcc tgcggcctgc gcggcaaacc cgatccattt ccgggccttg gactcgttct
     8761 tccactcccg gatcttctcc ctgcgctggt gctcgttcac gtcctttaag gagaggtcga
     8821 tgtggcccct ctgggtatcc acgtggagga ccttgcagac caccttctgc ccctcgcgga
     8881 tgtggtcgcg gatgtacttg atccaccccg tcgccacctc ggagatgggg atcaggccca
     8941 ccctgccggg atactcgtcg aggctgacga acgcggcgaa gtcttttacg tcctttaccg
     9001 tgcaaaccac cagttccgcc tgttcgggcc actctcgctc gctcatcgga ccaatcactc
     9061 gagcgtcgcc aggacttcag ccttgagctg tgcctttccg ccggatggct cggcaaggac
     9121 cttgccgcag accacgcact cgactgtcgt gctggccttc tcgaagacca cctgctcgtt
     9181 ctcgcagtcc gcgcacttga ccctgaagaa tttgctgcgg ttttccctga gttgtcgtac
     9241 catcttcctc actccgtcaa ttcgaacttg cctgcccgga acccgccgcg aaggtgtgcc
     9301 ttcttgcagg tgctgcaccg gtagcgcacg ttgatcttct tggtgggctt gtcccctccg
     9361 ggaaccttgg agaacttccc catgcttccc actgtgctcc tccttgcctt ctgcctgtcg
     9421 atccagtgca gtccggaggt cctgctcttc ttgaccttct ccacctcatg gacctggtgt
     9481 gagcggcaga atgggcagta tgtcttgaat ttgactggca ttttcataga tatggcctcg
     9541 tattcaggat atcgaatatc agtattattt gcccggattg atatttaaga ctatgttgcg
     9601 ctcacagagg acctcggcgt tccgctcggg aatggtcacc aggtcctcgc gctgcaggtg
     9661 gtaaatgtgc ccgtccacgc ccatgaacgg ctccatgtcc tgcaggaccc gtgagaccac
     9721 gaaggcaggc ctctcggcag cggcggcaag gaccgatgcg cagcggtccg cctccctctc
     9781 gctccccagg tgggagggcg cccgcggaac cgacccgtca accagccggc agcgggcctg
     9841 ctcgagggca aggaccacct ggtcaaacat gtccttctct tctggaagga gcttcttcag
     9901 ctcctcccgg tcgacatact gcccgtcctc ctgggagcgt gcgagggaga ggatcttctc
     9961 ggcccggatg cgatagaggt cgagcagcgt ctcgcgtatc gcggcgatcc gctcgatgag
    10021 gacccgcgcc tcgtcggaga acgggtcctc gcacgcgtag aggctggaat aatgcgtctc
    10081 caggcctgct ccgaccgagg aaaagaggtc tttctggatc tgcacgagcc ggcccgactc
    10141 gcgctccgag agcaggagga tccgcagttc atccaggtgc actggatgca cctcctgcga
    10201 tctccgccgc acccctgcag cagagaaaga ccgcgagcgc ctcggggacc gcggagatcc
    10261 ctttctccag ccggaaagag gtgccgagga tcggcatcga gagcggaaag gcgagatcga
    10321 ccttcacctc gcgctcgcca aagacgacgg catcgacgag gggatcgaag tcgtcaaggt
    10381 cctttggcgc aagcggggtc acccggagac cactcccgcc atggagcgat gacgggagcc
    10441 ggatcaggcg cttcgtgtcg gtcgtgaccg gctcgtccgc gagcgcggcc ctgtcccgga
    10501 gcatggcggc aaactggccc tccttctgta cggagagccc ccggatcacc tttgcaatga
    10561 ccgcgtcctt caggttgagg gacgcagggt cagtggtgat gcgggagagg acctcgccaa
    10621 ggcgccgcga gaactcctca gcagtcgtct tccccacccc ctcgaggccg gcaattgcgg
    10681 ccaccctctc cttctccccg aggctctcca gccataggag gtactcggca agtgcagcta
    10741 tatagcgatg atgccatccg cggggtgaat gtgtgcggtg ggcgaggacc cggcccgggt
    10801 caaggcctat cccgcacacg tagtcgatca gctcccgccg ctcgctgctg ccccagttcc
    10861 ggaccgagag gtcgtggacg tggacatggt agccccgtcc tcccgaaaag acgagatcga
    10921 tctcgcgggg cgaaaaccca agttccagta ccagcatgtc gaggagtttc tttgtctcct
    10981 ccttgacccg cgagagcatc ctgtcgtaag gcccgcgcat gatgtggtcg gcgtcaagat
    11041 cgaagataag gtcggccccg agccactcct tctcctgcat ggtccccgcc cccggcctgg
    11101 catagtacgc agtagagtag taaatgtgcg cgggggccat gctcctgatg taggagaagg
    11161 cctcgtcccg cgtctcaaag ccaaggtgcc tgcgcatccg gatgtcggga aaatcggggt
    11221 cgaagaagac gaaggcccac tccctctggg gaagggacga tggagagagg aggggctcct
    11281 ttgcgtaata agaggagaaa cgctgacgca ggaactcgag ggtggcggcc ttcatgaaag
    11341 tctcttgtgc atgtatgggc cccgccgctc atatccgtga cgccggtagt aaggcctcac
    11401 cccgatcccg ctcatcaccg cgatctcgcg gtaccctgcg gatcgcgccg tctcctccgc
    11461 cgtggagagg agtacccggc cgaacctccg gtgctgccgc tggtcatgcg cgggggagct
    11521 tccgatcggg acgatgctcc cgtacacgtg cagctcccgg aggagcgcgg cgtccacgag
    11581 ttcctcccgc caccgctgcc ccccgcaccg cagccgtgcg aacccgatca gggcatctcc
    11641 cgagaccgcc gagatgaagt gctcctttcc tccggtgcag gcataggaga aggtgcggta
    11701 ctccaccgcc ccgcctcccg cgctcctccc tgcctctcgg cagcggatgc agcggcagcg
    11761 cccgcccatc gcggccagcc gctgctggca gagctggcgg aaattgctgt gccgtgaccc
    11821 ggctacgatc agtttcgcgg ggatgtcgcg ctggatccgc tggagccgga cgtactcggg
    11881 gaggagcgac tttccgtatg ccaccaggcc gatcaggtcg tcctcgtcgt agggagcgta
    11941 cctccccctc atccagagcg actcgatctc ggatcccggg gtgacaagtg tcgggtagat
    12001 cttcaggaag tccggccgga agtcagggtt ttcgaagagc tcctggaaca tccaccggtc
    12061 acgctctatg ctgctccccg ggaggttggg catcacgtgg aacccgacct tgatcccggc
    12121 gtcccgcagc agccggtttg cggcgatggt atcctccacc gtgcagcccc tgcggttgta
    12181 ggcgaggatg cggtcgtcaa ggtgctggac gccgagctcg accttggtta ccccgagcgc
    12241 aagcatccgt tcgacgtgct cctcccggca ccagtcaggc cgggtctcaa aggtcgtggc
    12301 gacgcatcga accggcgaca tctcgttctg gaggaagacg gatgcggtat cggtcgcggc
    12361 ccccgccggg tgcccacggt actcgttcat ggcccggatg caggaagcca cgaaccactc
    12421 ctggtactcg gggtccctcg cggtcatcgt ccctcccatc acgatcagtt ccaccttgtc
    12481 cacatggtgg cccaggagct cgaactgctc gaggcgcgcc cgcacctggg agtaagggtc
    12541 ataaccgtgc tcccgggcac gaagcgccgc gggttcctcc ccggtatagc tctggggaga
    12601 ctggaacggg tggtcagggc cacccgggca tgggagacac ttgccgtggg ggcaggggga
    12661 gggagatgtc atcacggcaa ctggtgcgac accagagagg gtgcgggtcg gtttcacgag
    12721 gaggagtgcc ctgagccgct cccgttcgcc tggcagggcg gcggcaagga gagccgagtt
    12781 cttcgggacg gctgcggctc cgtatttttt gcagacctct atttttatcc gcgtgatgtc
    12841 tcccggggcg gcgtcccggg aaaaaattgc ctcgagtatc tcccgggaga tatcggggcg
    12901 tgccatgcaa ggttagtgtt cggctgcgac cttctctgat gcagtctcgg tggcgtaacc
    12961 caccggcacc atctgggtgc tctgaaggtg agtgacgatc tcgtcgccca gggcaaggat
    13021 tgcgtccttg tggcacttct tatttttgtg gatatgaaca ggagagatat gaagggagtt
    13081 atagcgtttt gtggggattt catggctggt gatggtttcg tagtacttct tgatgtggat
    13141 catcaacatg tggagatgga gtaattcttc cttttgcaca ctatcacctt ctgagaatca
    13201 acactatttt cctgatcaaa tatagtttat tggtgaactt tatatccgac atcaggcact
    13261 ccactgccgc cctgctgggg cttgcaattg gcgacgcgat gggtgcgccc ctggaggggg
    13321 tggtgcgccc ggcggccccc gtcagaagga tgtacgcggg gggcaggatc gcgcggcatt
    13381 ccggggcctg cacggacgac acctaccagg cgcttgcact cgcggagtcg cttgcggcgt
    13441 gccgcggctt ctctcctccg gacttcatgg aaaggctcgt ccgggactac ctctttttcg
    13501 gcgagtggta cggccccacc tcgggcgcgg tcttctccct ggtaatcggg gggaccccgc
    13561 tctattccgc ggcccgcctc gtgcacgccc ggcgggggtg gagcaggagc aacgggagcg
    13621 tgatgcgcgg acccccgatt ggggtgttct atttcggccc cgaggtcgag gccgcgtcca
    13681 tggcctgctc ggcactgacc cacagggacc cggtggcagg ggcctgctcg gcgtttgtca
    13741 accgcatggt gagcgacatg tgccgcggga tgcctaaaga gagggcgtat gcccgagcgc
    13801 tccagcggtg ccgggacgcg gaggtggccg gcatgctggg caggtggcgg gaattcccgc
    13861 cgtcccccgg actcgatgcc ctggaggcga cccatgccgc ggtctcggtc ttcatggaga
    13921 ccttctcctt tgaggaggcg gtgccgcgtg cagtcagcat gggcggcgac agcgacacgg
    13981 tcggggcgat cgcaggtgcg ctggccgggg ctgcctacgg catgcattcc atccccgaag
    14041 agtggatccg cggcctctct gacagcggcc gtgtgctgcg ggcggcacac gcgctgtggc
    14101 ttgcgtcccg tggctgatac cgatcagacc gccctcagtt ccaccagctg gaggcttcgc
    14161 cccttcacgc aacccggggg cgcattcccg agcaccctga cgacgacgta cttctcgccc
    14221 gggatgacgc cctcgggatt gcagagccgg aaatgttcgc acttatccct ggagcaggag
    14281 ggttcaaaca ggatgcgggt gtttgtgatg gccatgtccg cgctgacgag ggccgcaatc
    14341 gacgactcca ccacctcaac tgccgtggca ccctcaaggt gcagggggca tgcgtggtgg
    14401 gtcttcctca ccccgacgac ccggtatctg cgcccgggtt tcaggttatg gcacgccttc
    14461 ctgaccgtgc agccctcgca ctcggcgcac tcgccctcgt agatgaactc gaggtcgacc
    14521 ctcgcgaggg tggtgccgat gagggtgacc tttggtttga cttctgccat tctctcttac
    14581 tcccggtaga gcatcctcac gagctcggtt gcagactctc tcgtcagccc catgtccagg
    14641 atggtgaacc gctcgggccg gatatccttt gcggcaagca gcgcctcgac ggcgaccccg
    14701 tcgtcgatcc cgaggtctgc aggggtgatg ggtgcgccga tggaccgcag cgcctctctg
    14761 atcgcccgcc agtctccgcc gtgcaggtac atggcgatga tagtgccgat cccgcaggcc
    14821 tccccgtgga gggccgctcc cggtgcgagc cgctccaggg catggccaaa cttatgctcc
    14881 cccccagacc ccggccgtga ggagccggca atactcatcg cgacccccga ggatacgagc
    14941 gccttcgtga ccatccacgc ggactcctcc tggtggggct tgatgccctt tgcgttcttc
    15001 atcaggagct ccgcggtcat ccgcgagaga gcgaccgcgt actcgctcac ttcttcgtgc
    15061 ttcagccggt gggcgagctc ccagtcgagc acggcggtgg agttcgagat tacgtctgca
    15121 cagccggacg cgagaaggcg gtgcggcgcc gccgcgatga gcccggtgtc cgcgacgatt
    15181 gcaatcggcg gatgcgcctc gagtgagacg ctcccgctct ccatcgggac cgacgccctg
    15241 gcggacgcga taccatcgtg ggaggctgcg gtcgggacgc tgatgaactg gcggtcaagg
    15301 ttataggaga cgatcttcgc gcagtcgatt acccggcccc cgcccacgcc tgccaggaac
    15361 cccgcgcccc gtgagaactc ttctgcctcg tggattgtat ccatgcttat ctccctgacc
    15421 gaaaaagtgg agacctcgta gcgctccgag aggagggatg ccacatggtc gcccgcacat
    15481 tctcgggtcc gggtccccga gaagaccacc acgcggccct tcaggcagag gtctgcgcag
    15541 accgagggga tctgggggag gacgtcgtgg ccgatgacca cgtcccgggg gagctgcatc
    15601 caccgggatt tgtcaaagac ctttgtttta agtactttta tcgggtctgc gctcatatga
    15661 atcaggaaat gatctgggat ttcatacaga aatattacat tgatccaata cgctatggcc
    15721 agccctacaa cgtggtggat acgactactt acgctctggt tctgatcctt gccatatata
    15781 tcatttatcg ctggctctcc aggtccgaga tcatccggat cgaccggaaa ttcattctct
    15841 gcaccctccc gttcgtcgtg atgggagggc tgctcagagt ggtgcaggac acggggatga
    15901 tcgagtcgga ctggcagtac ctgctgatca ccccgctgat ttacttcgtg atgttcgcgt
    15961 acacggcagg ggctctcgtc atctccgtga ccctccagga gcacggggcc ctgaaagatt
    16021 atcacagggg ataccttgca gcgggaattg ccggggcggc ggcgaccggg cttgtcctcc
    16081 tctggttcgg cgcagagaac gggatcatcg ccctggatgt cctcgccatc atcctcgcga
    16141 tggcggtcgc aaccaccgcg gcggtctacg cagccatgcg ctacctcctg aagtgggagt
    16201 atgtcgccga ccccctctac ctctccctcc tcttcggcca gctcctcgac gcgagcgcca
    16261 cgagttacgg gatcgacctg cacccgctcc attacgtcga ggtgcacgtg gttggctcgc
    16321 acctcatcga gtggacgggg acggcgttct cgatgttcgg gttgaaactc ctggtcctct
    16381 tccccggcat ctacatcctg gagaggttcc gccacgaggg tcctggcgcc ctctggcacc
    16441 ttatcctcct tgcgatgatc acagtcgggc tggcgccggg aatccgggac atgatgagga
    16501 tggtgcttta tgtctgacct ttccaggaac gcgggcattg tcgtcatcct tttggccgta
    16561 tcggtcgcgt tcggggcatg ggcgacgatg caggaccccg cggtagggga gcagctgatc
    16621 tcgctcctca aggagtccat catgggagag gcgctcgatt catcgggggc gatgatggcg
    16681 gtgatgctct tcttcaacaa cctccaggcc tgcgtcctca tgttcatcgg cggggcgtcg
    16741 ctcggcctgc tcacggtctt catcatcagc acgaacggct ttgtgattgg gtctgtcctc
    16801 gagatggtga gggaggagca cagcgccctt tacgtggcgg ccgcgatcat cccccatggc
    16861 atcttcgaga tcccggcgtt cgtggtatcc ggggcgctcg ggctcatgct ggcaaaggcg
    16921 ctctggaagg aatgggaagg aagcgaggac gctgcttccc gggcgcttgc ctatgcccgc
    16981 acgttcctgc tggcagtcgt ccccctcgtc gcggttgccg cgtgcgtaga ggcatttatt
    17041 acgcctgaaa tcctacgttt agcaatttga ggtgtgtttt ttggccgagg aagatcaggg
    17101 ggcgctcccc cccaagggtg acttctctgc atggtacaac gagctcctgt ggcgggcgga
    17161 ggtcatggac gtccggtacc cggtcaaggg cctctacgtc tggtacccgt tcgggttctc
    17221 gatcaggcga aacgtctacg atatgctgcg caggctgctc gaccgtgacc accaggaggc
    17281 cctcttcccc ctgctcatcc ccgagaacga gcttgcgaag gaggccgaac acatcaaggg
    17341 attcgaggac gaggtctact gggtcaccca cggcggcctc accccgctcg acgtgaaact
    17401 tgcgctccgg ccgacgagcg agtgcgccat ttaccccatg tacgcgctct gggtccgctc
    17461 ccacgccgac cttcccctga agatctacca gatcgtcaac acgttccggt acgagaccaa
    17521 gcagacccgg ccgatgatcc gcgcccggga gatcacctcg ttcatggagt cgcacaccgt
    17581 ccacgcgaca tgggacgagg cggaaacgca ggtcgaacag gagatcgcgc tcaccctcga
    17641 gttctacgat tcgctctgca tccccctcat catctcgaag cggccggact gggacaagtt
    17701 ccccggcgcg gactacacgg tcgcggtcga cacggtgatg ccgaacggaa ggactctcca
    17761 gatcggcacc gtccatcacc tcggcacgca cttctcaaag acgtttggca tcagtttcga
    17821 ggatgagaag ggagcgcaga agcctgcgaa ccagacctgc tacggcatct cagaacggtc
    17881 ggtcgctgcc cttatcggca tgcacggcga cgaccgggga ctagtccttc cgcctggccc
    17941 cgcacctgtc caggtcgtga tcattcccat tacgatagga aagcggaaag acgaggtggg
    18001 cgcggcagcg gcgggaatcg agaaggagct ctctgcagcg gggttccggg tgaagatcga
    18061 ttcacgcgac atccgccccg gggccaagta ctactactgg gagatgcggg gcgtccccct
    18121 ccggctcgag gtcggcccaa gggacatcga tgccggccag gtgaccgccg tgacccgcct
    18181 cggcgagaag tcccagatcg cgagagatgc gcttgttgaa ggggttaccg ggcggctcgc
    18241 ctcctttgca gccacgctga aggagcgggc ggaggctcac gtccgcaccc acatgagggt
    18301 ctcgcggagc atggacgagg tggcggccgc ggtcgaggac ggcgtcaccc tcgttccctg
    18361 gtgcggcagc cgcgtgtgcg ccgatctcat cgaggagaag accagggccg cggtcctcgg
    18421 gaccgaagtc cggtccgcgt acctcgtgga cgcctccggc ccgtgcgtcg cgtgcgggaa
    18481 gcaggggacg actgcgctgg tgggaaggtc gtattgagca gttggggcat cagccccaac
    18541 tgcggggggt gtgcggggga ggcgccggag
//
LOCUS       DAOQYR010000040        18081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_3490_length_18081_cov_6.733773, whole genome shotgun sequence.
ACCESSION   DAOQYR010000040 DAOQYR010000000
VERSION     DAOQYR010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 18081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18081
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3490_length_18081_cov_6.733773"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..980)
                     /locus_tag="PKM87_06260"
     CDS             complement(24..980)
                     /locus_tag="PKM87_06260"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNQ29706.1"
                     /translation="MEDIVTNSIMERLKNPIRKVIPKKIRLYFFYRNVEREANNFGVS
                     IKKIGINYSIRKDREEIRVSVEHSIYLYDIIQNFEFYFSGVKPIRWHDTDIVDFSTPR
                     LHWVTGFEYFPILFPSFVEPIITTKQYIEFAALRSDSVVIDLGAYSGLTSILFDMAIP
                     TGGQVISIEADRNNAVTCRENLHLYERFFKRKIKFLEMAIWKNDLGVNFSSEGNMGSS
                     VIDIVGPSRGENIIVPSLTLTQLAHRLKLEKIDFIKCDIEGGETEIFDAPEFFSEYLP
                     KILIECHFIDGKRTTENRCREVLEKFGYTCDTAHIPQVEFQK"
     gene            complement(1062..1949)
                     /locus_tag="PKM87_06265"
     CDS             complement(1062..1949)
                     /locus_tag="PKM87_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNQ29707.1"
                     /translation="MRVFLTGGNGFIGRNLVEHLSGSMEIVAPGSRDLDLTDQDAVER
                     FVNTGDFDVVLHTATWNATANSPKDTRMVLEKNLIMFFNLARLHERFDKMVYYGSGAE
                     YDRRYYQPFMREDYFGRYIPVDQYGLSKYVMAKYTEGNAENIVDLRIFGCFGPYEDWE
                     IRFISNAMCKALYGLPITIRQNVFFDYIWVTDLVRMTEWALTIRTKYKHYNACTGRHV
                     DLLTYARMINDVTGADVPIQVGKEGLKPEYSGDNSRIMSEMGGFEFTPLRTALVMLKK
                     YYEGNLSNIDRHSLLVDKG"
     gene            complement(1951..3750)
                     /locus_tag="PKM87_06270"
     CDS             complement(1951..3750)
                     /locus_tag="PKM87_06270"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNQ29708.1"
                     /translation="MIKVSDYIVKVLERIGVDTVFIVTGGGAMHLNDSFGQSSRIRKI
                     FNHHEQACAIAAEGYARIAERLAVVNVTSGPGGLNTLTGVMGQWTDSVPVLYLSGQVR
                     YDTTVESQKGRLDMRQLGDQEVDIISVVRPLTKFAATVKDPHDIRWILEKAISEATTG
                     RPGPVWVDVPHNIQSATVDEHRLLGYDTGVPPDTQRLQGQVQHVIRLLAAAERPIVVC
                     GRGTRISGTIDLVRTFCSNNNIPIVTTFNNYDAVPDDFPLYSGRIGTVGQRAGNFCLQ
                     NADLVLFLGTRNNIRQVSYSWNFFCRAAIRIAVDIDYSELRKPLVTYDLAIRADLGDF
                     MPILLNDAHRATFPDWKWWVEWCQERRLRFPVVTQEQRESERLNPYHFIHELTKMAGP
                     DVVIVAGNGTASLGLFQSSVVHYGQRMLNNSGCAAMGYDLPAAIGAACACGHDVVCLA
                     GDGSFQMNLQELATISYCQLPIKIFYLDNGGYASIRQTQDTFFGRRYGINRQTGIGFP
                     DTKKLADAYGLHHYEIKEPSDMIETLRKVLKADGPVLCHVFLDPAHIFEPKLSSARNS
                     EGQLVSKPLEDMYPFLPRDELKENMIIDLVPGD"
     gene            complement(3734..4828)
                     /locus_tag="PKM87_06275"
     CDS             complement(3734..4828)
                     /locus_tag="PKM87_06275"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNQ29709.1"
                     /translation="MKSELFNDLFVLELANNHWGDLRRGLLIIRTFAKLVRKYEIKAA
                     IKLQFRNASTLIHKDYKGRTDIRYIHRTEARILSEHAYRRMVDEIRAQGMITMSTPFD
                     EESVDFCEDLDIDIIKIASFDLNDWPLVNKVLEKRRPTIVSIGGTGIDDIDRIVNLFN
                     ESKVPLAINQCVSIYPSEPEELELNQVSLMVKRYPDNVIGLSSHEYQDFSLSLAIAYG
                     MGARTFERHIDIPHPVHMAAYNMLPEQCETWFRTYKLIKLMCGDPSDTLRVPPCKEID
                     YINEHVRGLFAKRDLARGDTVSLDNFYMAVPVHRGQMSCREVMWGGTLARAVKKDSPL
                     MVDSIHADYLKDEQLIRRINERGRIFDKSQ"
     gene            complement(4818..6191)
                     /gene="rfbH"
                     /locus_tag="PKM87_06280"
     CDS             complement(4818..6191)
                     /gene="rfbH"
                     /locus_tag="PKM87_06280"
                     /inference="COORDINATES: protein motif:HMM:NF011936.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HNQ29710.1"
                     /translation="MVNPSEKEKVLRSTAIRAALDHFHEYHAKEDYKEGDKIQYGGRV
                     FDEQEIRYLVDSCLDFWLTSGRYADRFEHGLGEYLGVKYVSLVNSGSSANLLAFTALT
                     SPKLGERRILPGDEVITVAAGFPTTVAPILQYGAVPVFLDITLPGYNIDTSLLDKAFS
                     DRTRAVMLAHTLGNPFDLERVKDFCDQNGLWLIEDNCDSLGSRYFYQGEWHYTGTIGD
                     LATSSFYPPHHITTGEGGAVYTNNRELIRIVNSFRDWGRDCQCAPGQDNFCGKRFMQQ
                     FGELPYGYDHKYVYSHFGYNMKATDMQAAIGCAQLEKLPGFTEARKRNWKFLYDSLSD
                     LGHYFVLPEPARNSDPSWFGFMLSVKFGVDISRDSLVDYLESKGIQTRMLFAGNLIKQ
                     PCFNEMRKKKQGFRVVGDLKNTDLIMSQTFWIGVYPGLRQVQLDFMVQCIRNYILDLA
                     PEVGHEI"
     gene            complement(6320..7405)
                     /gene="rfbG"
                     /locus_tag="PKM87_06285"
     CDS             complement(6320..7405)
                     /gene="rfbG"
                     /locus_tag="PKM87_06285"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HNQ29711.1"
                     /translation="MSAYKELADAYQDKTVFITGHTGFKGAWLSLWLESLGARVIGYS
                     LDPPTIPSLFDVLQLGDRIIDIRGDIQDRNTLLDQIKKYRPEYIFHLAAQPLVRLSYQ
                     NPLETFAINVIGTVNVLESIRTLRFPTTCVCITSDKCYENRGVVHAYQETDPLGGHDP
                     YSASKGAAEIAISSYRKSFFHNGKGEPLVSLSSARAGNIIGGGDWAPDRIVPDCIRAL
                     VKGEPVVLRNPHAVRPWQYVLDPLYGYLLLGLKMQEKPREYAEAWNFGPHHGNAVDVQ
                     TLVENLLKEWGSGTWELDAHQERRPHEAAYLLLDISKSKERLGWDPSYDITGGIRRTV
                     EWYKEYYAQSGDINEYSLGLITEFYRH"
     gene            complement(7405..8184)
                     /gene="rfbF"
                     /locus_tag="PKM87_06290"
     CDS             complement(7405..8184)
                     /gene="rfbF"
                     /locus_tag="PKM87_06290"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HNQ29712.1"
                     /translation="MVHTKVVILAGGMGTRLIEETVVRPKPMVEIGGKPILWHIMKIY
                     SYFGFNDFIICLGYKGYMIKEYFANYYLHMSDVTIDLNRNTTEIHHNYSEPWKVSLID
                     TGDNTMTGGRVKRIQQYIGNNRFMLTYGDGVSDLNIHELLSFHEKNGKIGTVTAIRPA
                     GRFGALDIRENTVISFAEKPRGDGDYVNGGFFVFEPDIFDYLENDATILEREPLESLA
                     KKRQLGAFKHEGFWYPMDTLRDKNYLEGIWATGKAPWKLWD"
     gene            8350..9375
                     /locus_tag="PKM87_06295"
     CDS             8350..9375
                     /locus_tag="PKM87_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 10"
                     /protein_id="HNQ29713.1"
                     /translation="MTKIGFYNYYNIYNNNRMFNPEIQAPLGEDLLYPMHYLARVAKE
                     KGISVSTIDTEPLDSYDAIFFIDFPGYGNRYFRKLLSMKFENMYLFLCENENVKPDNW
                     RKENYRHFKKVFSWNDRLVDNKKIFKFFLANKIPTPFSFDVSEKTKFCCMIAANKWND
                     LPGELYSERVRAVRWFEQYHPESFDLYGKGWDFQTPSFFNPLRPYLKQIRNFFPPKYP
                     SYRGEVASKRAILSQYKFSICYENMRDVPGYITEKIFDCFFAGCVPIYLGASNVTEYI
                     PEYTFIDKRKFSDYPELYEFLINLSDSAYRKYLHSIEEYIQSDKISPFGAEYFVNTIL
                     NEVISSA"
     gene            10527..13205
                     /locus_tag="PKM87_06300"
     CDS             10527..13205
                     /locus_tag="PKM87_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006487 - protein N-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharyl transferase, archaeosortase A
                     system-associated"
                     /protein_id="HNQ29714.1"
                     /translation="MRLPELKITRKCLFILVILFILTLFTLWIRVLPLLTLNTDILNI
                     VAADDPMYSLRQIEQMLANYPAYTWYEAMSLYPFGQDIPWGPLFTWITTTIVMICGAA
                     TRDEIISVALWIPPLMAAVMVPVMFVLVRKLTDWKAGLCAAVLIAVVGGQYFFRALAG
                     YLDHHVAEVLFSTIFCIAYIYALAYFREQAVDLKNKETWKVPVFLSVIAGIGYFLGYL
                     TMSTMILFAFIAALSTIFISVVDFYHGKSGEYMVLLNTVSFGLAAVLSLLVSIISGGF
                     DEGFSFNLYTLLHPVSYLLLVIGTWVLYWLARNLKGKSQILYPVSVIGLGFIAFLSLY
                     LFIPGLYGSFVSGIEVFFGLNPYAVTVQEARPWSLVEAWQVFNFGLILMAAGAFVLLY
                     RSWKEYRPQYIFVMVWSVFIIVAAFRQVRYEYYLAVNIALLGGIALGFFLDLAWPDIC
                     AMGRRVTANEARKGPAEETALAKEEERKPELKSEKGKGKKSGKKQEPSHKTVEKPEKA
                     SKKAAPETRIRYIHVLVAALFCACALLFAVGSVQYEYAVSASGGIRMNPDWRESLEWM
                     EAHTPDTGVDYLRIYDENTFKYPDTAYGVMSWWDYGHLITYIAKRIPNANPFQSGVAG
                     ENGAATFFLSETEERNTAIADTLGTRYVMTDIEMSSGKFWAMATWYNSSAGQAPYVKT
                     VLAPEEGPQGTYSAVSLYDASYFNTMIARLHNFDGSMVAGGQAYYVEVDDRLSSYPVA
                     TAAQMMDTAVARAAADAYNQKAAPGTRAMVLSSNLLTPIDTVPALKHYRLVHESPTNV
                     IPAGAGWDIKYVKVFEYVPGARIQGTGVIALDLVSNTGRTFTYKQASTDGEFIVPYST
                     TGSPYEVKAAGRYRIEGTGREIDVPETAVMQGLQVG"
     gene            13206..14186
                     /locus_tag="PKM87_06305"
     CDS             13206..14186
                     /locus_tag="PKM87_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNQ29715.1"
                     /translation="MASQCSVIFGEEFTRHDLGWHDENSRRLTRALECIPKNYKVRPV
                     EEASYDDLARVHHPRHIRMIEELCGYGGRRYLDMDTYITEDSYQVALYAAGSAIAATR
                     LALEGEHCFALIRPPGHHAEPDKAMGFCLFNNAAVAAAWALERVDRVAIVDWDLHHGN
                     GTQKIFYSSDRVLFCSIHQQNVFPWSGWIDEIGEGRGRGFNLNAPLPAGGGIGDYALI
                     FSEVFSDALSHFHPDVVIVSAGQDPLEDDPKGGMRLTPEDFGLLAALIRDACHQPLAL
                     LLEGGYGPSLGDAIACILAVLNEGRTPEIPDVPERESTRRTARLLRRVMI"
     gene            complement(14204..15418)
                     /locus_tag="PKM87_06310"
     CDS             complement(14204..15418)
                     /locus_tag="PKM87_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HNQ29716.1"
                     /translation="METIKLFPANGVVMECELNRVEKRYFHGTHRTCTPKETEKMARP
                     LMKSIGAGPVRDITRSDRVGIPCCVSSRSRIPRGGTGVHPGMGLDLPLARVSAMMAAI
                     ERFSGEYHGDQMVYASYEEAGVARAVDPESLILPRPLEQGEKLHWTPGADIMNCEQVM
                     VPSNAVFFPYDTLGMTMPLFQSDPSGLASGNVREEAILHGLLELLERDAMSRAERKRD
                     MGKRLVLDTGGPGRALLDRFTSAGVAIHLWLLEGRTRVPVVAAAADDQVTRDPALLMM
                     GSGCHTDPEIAAARALTEVAFSRAAYLREGTTSSHREMLLSRAGYERMKRINREWFAD
                     APEIPVSSLPDVSSQLIDEDISLIIDDLRPGVERVCVVDLPRTAVPVVRVVVPGLEVS
                     HLHKDRKVRQQG"
     gene            15435..15728
                     /locus_tag="PKM87_06315"
     CDS             15435..15728
                     /locus_tag="PKM87_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2098 domain-containing protein"
                     /protein_id="HNQ29717.1"
                     /translation="MQADEVEVGMRVRYPRTGTTGRVVALENGMGGLFAELDSTHLRY
                     RVDQLVPAGVAGERARTTKEDLMDRLKKEREFLSATGFQDAISHTDQSCEGGG"
     gene            complement(15725..16147)
                     /gene="nikR"
                     /locus_tag="PKM87_06320"
     CDS             complement(15725..16147)
                     /gene="nikR"
                     /locus_tag="PKM87_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HNQ29718.1"
                     /translation="MTIDTELSRIGISLPKNLLDKFDEIINYRGYSSRSEGIRDAIRN
                     YITYYKWMSDVKGERQGVITMIYDHEQRNLLATLTDIQHEFMGIIQASLHSHVTHDKC
                     LEVVLVRGDGSELKTLAERLMSQKGVESVKLTTIPITD"
     gene            16316..16972
                     /locus_tag="PKM87_06325"
     CDS             16316..16972
                     /locus_tag="PKM87_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29719.1"
                     /translation="MSAPSYHAHSEKGLRERNEDACMAREVGGIHLFAVAEGMGGSPE
                     GPSAAEVAISALCSGESVYAGSLADMAESFLARADDALFHSHESNPVADPPAVAMTLA
                     IVGPGGECLVSSPGQRKVFFMPVPPGENKGPDPNDIQDSPVLHDPGAGMHEAVLPPGF
                     LILCSDGISEFVTDDRISEIVRERGDDLEGACRKLVYEAFQNGSNDNLTMVLVRQPDR
                     "
     gene            17047..17937
                     /locus_tag="PKM87_06330"
     CDS             17047..17937
                     /locus_tag="PKM87_06330"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNQ29720.1"
                     /translation="MDSITHGLVAMLAFHATFPGLLVLFAVLGAVLPDSDVFLSLLSD
                     RDPRLFVFTHGGFTHSIPGSAAIAFGVAAGFQAWLCLAGQGHVGGTFLVLAWALAWAG
                     ALSHIALDALAFPGIPLLYPASTRKYSAGIFPGPSLVLFGTSLGFLLMHFAGYAGTGA
                     LYAYMAFIGVFVAAHVILKAFVAARHPGLTIPTFNPMKWLVVREVEDSYEVCSAGLSR
                     ECTSIAVFPRYDGVRPEYLDRHSNDPEMARVTYHSYITVAARRDGSIVVRDPLRDSGI
                     LRYPPYYRSVRLPEEERGTP"
BASE COUNT         4124 a         4755 c         4641 g         4561 t
ORIGIN      
        1 ctggggccgc cgtatgaaaa atgttatttt tgaaattcga cctgcggaat atgggccgta
       61 tcacaagtat acccgaattt ttcaaggact tcacgacatc tgttctctgt tgtccttttt
      121 ccgtcaataa aatggcattc aatcaatatt ttcggtaaat attctgaaaa aaactcaggt
      181 gcatcaaata tctcagtttc ccctccttcg atgtcacact tgataaaatc aatcttctcc
      241 aatttcagtc tgtgagctaa ctgagttagc gtgagagaag gcacaattat attctcccct
      301 ctcgatggac cgacgatgtc tataaccgaa cttcccatat ttccttctga agaaaaattt
      361 actcccagat catttttcca gatagccatt tccaggaatt tgatttttct tttgaagaat
      421 ctttcataca gatgtaaatt ctctctacat gtaacagcat tattcctatc ggcttctatt
      481 gagattacct gtcccccggt tggaattgcc atgtcaaata agatacttgt taatcctgag
      541 taagccccca gatcgattac gacggagtca cttcttagcg cggcaaattc gatgtactgt
      601 tttgtggtaa tgattggctc tacaaaagaa gggaaaagaa taggaaaata ctcaaaacct
      661 gttacccaat gcaagcgagg cgtagaaaaa tcaacgatgt cggtatcatg ccatcgaata
      721 ggtttaaccc ccgaaaaata gaactcaaaa ttttgaatta tatcgtataa atagattgaa
      781 tgttccacag atactctgat ctcctctcta tcttttctta tactgtagtt aattcctatt
      841 ttttttatag agactccaaa attattcgct tcgcgctcaa cattgcgata aaaaaagtaa
      901 agacgtatct tttttgggat aaccttgcgt ataggatttt tcaatctttc catgattgaa
      961 ttggtaacga tatcctccat gagttaatct ccaatcatta tgaaattacc aatacaaatg
     1021 atccctctca ttggttgatt ttattctcag accttgaatt cttatccctt atcaaccaat
     1081 aaagaatgtc tgtcaatatt actcaggttc ccctcgtaat atttcttgag catcaccaat
     1141 gccgttctca atggtgtgaa ctcgaaccca cccatttcag acatgatcct tgaattatcc
     1201 ccgctgtatt cgggtttgag tccttctttt cccacttgaa tgggaacatc ggcaccagtg
     1261 acatcgttta tcatgcgggc ataagtaaga agatcgacgt gccggcccgt gcatgcattg
     1321 taatgcttgt acttcgtgcg tatcgtcaat gcccattcgg tcatccgaac gaggtccgtc
     1381 acccagatat aatcgaaaaa tacgttctga cgtatagtaa tgggcagccc gtagagagcc
     1441 ttgcacatcg cgttcgatat gaacctaatc tcccagtcct cgtaagggcc gaagcacccg
     1501 aagatccgca aatccacgat attttcggca tttccttctg tgtactttgc cattacgtat
     1561 ttcgacaggc cgtactggtc gactggaata tatctgccga agtaatcctc gcgcatgaaa
     1621 ggctgataat agcgccggtc gtattcggcc cccgacccgt agtataccat cttgtcgaac
     1681 cgttcgtgca gacgtgccag gttgaagaac atgatcaggt tcttttcaag gaccatccgg
     1741 gtgtccttag gagaattggc cgtggcattc caggtcgcgg tatggaggac gacatcgaag
     1801 tcgccggtat tgacaaaccg ttcgaccgcg tcctggtcgg tgagatcgag atcgcggctg
     1861 cccggtgcaa cgatctccat gctgccggaa aggtgttcga cgagattgcg gccgataaaa
     1921 ccgttccctc cggtgaggaa cacgcgcatc tcaatctcca ggtaccaggt cgatgatcat
     1981 gttctccttc agttcgtccc ggggtaaaaa cgggtacatg tcttcgaggg gttttgacac
     2041 cagttgaccc tcggagttgc gggcagacga gagtttcggc tcgaatatat gggccgggtc
     2101 caggaatacg tggcataata ctggcccgtc ggccttcagg accttcctca gcgtctcaat
     2161 catatcgctg ggttctttaa tctcataatg gtgtagaccg taagcatccg ccagtttttt
     2221 tgtatcggga aacccaattc ccgtctgcct gttgatcccg taacgccgtc caaagaacgt
     2281 gtcctgagtc tgccggatgg acgcataccc cccgttgtcc aggtaaaaaa tttttatagg
     2341 aagttggcag taggagatcg tggcaagttc ctggaggttc atctggaagc tgccgtcgcc
     2401 ggcgagacag actacatcgt ggccgcaggc gcaggccgca ccgattgccg cgggaagatc
     2461 atatcccatc gctgcgcagc cggagttgtt cagcatcctc tgcccataat gcactaccga
     2521 tgactggaac aggccgaggg aggcggtgcc attccccgca acaatcacta cgtccgggcc
     2581 tgccattttt gtcaattcgt ggataaaatg gtaaggattc aatctctctg attctctctg
     2641 ctcctgggtt acaaccggga aacgcagcct tctctcctgg caccactcca cccaccactt
     2701 ccagtccgga aaagtcgccc ggtgcgcatc attcaggaga atcggcatga aatcgccgag
     2761 atctgcgcgg attgccaggt catatgttac cagaggtttg cgcaattcgg aatagtcgat
     2821 atcgaccgca atgcgtattg cggcacggca gaaaaagttc cagctgtagc ttacctggcg
     2881 gatattattc ctcgttccaa ggaagaggac gagatcggcg ttctgcaggc aaaaattacc
     2941 ggccctctgg ccaacagtcc ctatccgtcc ggaataaaga ggaaagtcat cggggacggc
     3001 atcgtaattg ttgaacgtgg ttacgatggg tatgttgtta ttgctgcaaa aggtacgaac
     3061 aagatcgatc gtcccggata tacgcgtacc tcgcccacat acgacgatag gccgctctgc
     3121 tgcggcgagc aaccggatga cgtgctgcac ctgtccttga agtctttgcg tatccggagg
     3181 tactccggta tcgtagccga gtaaacggtg ttcatcgacg gttgcggact ggatattgtg
     3241 agggacatcc acccagaccg ggcctggacg cccggtcgtc gcttcggaaa tggctttttc
     3301 caggatccac ctgatatcgt gcggatcctt cacagttgcc gcgaacttgg tcagggggcg
     3361 gacgacggat atgatatcca cctcttggtc gcccagctgc cgcatgtcga ggcgaccctt
     3421 ctgggattcg acggtggtat cataacgtac ctgtcccgag aggtataaca ccgggacgga
     3481 atcggtccat tggcccatca cgccggtgag ggtgttgagg ccgccggggc cggaggtgac
     3541 attgacgacc gccaaccgct ctgcaatccg ggcataccct tcggcggcaa tggcgcacgc
     3601 ctgttcgtga tgattgaaga tctttcgtat ccggctcgac tgcccaaagg aatcgttcag
     3661 gtgcatggcc ccgccaccgg ttacaatgaa cacggtgtcg actccaatcc gttcgaggac
     3721 tttcacgata tagtcactga cttttatcaa aaatcctacc tctctcgttg attcttctga
     3781 tcagttgttc gtccttgaga taatcggcat gtatgctgtc aaccatgagt ggagagtcct
     3841 tttttacagc cctggccaac gtcccgcccc acatcacctc gcggcatgac atctgtcccc
     3901 tgtgaaccgg gactgccata tagaagttgt ccaggctgac agtatcccct cgtgccagat
     3961 ccctctttgc gaacaaacct cttacatgct cgttgatata gtcgatctcc ttgcatgggg
     4021 gcactcgcag cgtgtccgag ggatcaccac acatcagttt gatcagcttg tatgtacgga
     4081 accacgtctc acattgctcg ggcagcatgt tgtaagccgc catatgtacg ggatgcggga
     4141 tgtcgatgtg ccgctcgaac gtccgggcgc ccattccata ggcgattgcc agcgagagcg
     4201 agaagtcctg gtactcatgg gaggataacc cgatgacatt gtcgggatat cgtttcacca
     4261 tcagcgaaac ctggttcagc tccagctctt ccggttcgga aggatagatc gagacgcatt
     4321 ggttgattgc gagggggact ttcgactcat taaagaggtt cactatccga tcgatatcgt
     4381 cgattccagt accccctatc gagacgatgg ttgggcgccg cttctcaaga accttgttga
     4441 caagaggcca gtcgtttaag tcgaatgagg cgatcttgat gatatcgata tcgagatctt
     4501 cgcaaaaatc gacggattcc tcatcgaacg gagtggacat ggtaatcatg ccctgtgccc
     4561 ggatctcgtc caccatccgg cgataggcgt gttctgataa gatacgtgct tccgtgcgat
     4621 ggatgtaacg gatgtctgtc ctgcccttat agtccttatg gatgagggtt gacgcattgc
     4681 gaaactgcag cttgatggct gcttttatct cgtattttct gaccagtttc gcaaaagtcc
     4741 tgatgataag aagtccgcga cgcagatccc cccagtggtt gttggccaat tccaacacga
     4801 acaggtcgtt gaacagttca gatttcatgc ccaacctcag gcgccaggtc cagaatgtaa
     4861 ttccttatgc attgtaccat gaagtcaagt tgaacctgtc tcaatccagg atagactccg
     4921 atccagaatg tctgggacat gatcagatcg gtattcttca aatcgcccac cacccggaaa
     4981 ccttgttttt tcttgcgcat ctcgttaaaa caaggttgtt ttataaggtt gcccgcaaaa
     5041 agcatccggg tctggatgcc tttcgattcc aaataatcga cgaggctgtc cctagaaatg
     5101 tcgacaccga attttaccga gagcataaac ccgaaccagc ttgggtccga gtttcgcgca
     5161 ggctctggaa gaacaaaata gtgacctaga tcggacaggc tgtcgtataa gaatttccag
     5221 ttcctcttcc tcgcctcagt gaacccgggc aatttctcaa gctgggcaca accaatagcg
     5281 gcctgcatgt ccgttgcctt catattgtag ccaaaatggg aatagacgta tttatggtca
     5341 tatccatacg gcagctcccc gaactgctgc atgaatctct tcccgcaaaa gttgtcctgg
     5401 ccgggggcac actggcaatc gcgaccccaa tccctgaaag aattgacaat acgtatcaat
     5461 tcacggttgt tggtatagac tgcccctcct tcacccgtgg taatgtggtg tggagggtag
     5521 aaagaagagg tcgcgaggtc tccgattgtc ccggtatagt gccattctcc ctggtaaaaa
     5581 tagcgcgaac ccagtgaatc acaattgtct tcgataagcc agaggccgtt ttggtcgcag
     5641 aaatccttta ctctctccaa atcgaaaggg ttgccaaggg tatgggcgag cataaccgct
     5701 cttgttctat ctgaaaatgc tttatcaagt aggcttgtat caatgttgta tccgggcaag
     5761 gtgatatcga ggaagaccgg caccgcgccg tactggagta ttggtgcgac ggttgtcgga
     5821 aacccggcag cgacagtgat aacttcatct cccggcagga tgcgcctctc tcctagcttt
     5881 ggggaggtca aggccgtgaa cgccagcaga ttcgccgaag aacctgaatt taccaaagag
     5941 acgtatttca cgcccaggta ctctcccagg ccatgctcga agcgatcggc ataacgacct
     6001 gatgtcaacc agaagtccag gcaggaatca acgaggtatc gtatctcctg ttcatcaaat
     6061 accctgccgc cgtactggat tttatctcct tctttatagt cttctttggc atgatattca
     6121 tggaaatggt ccagagcggc ccggatcgcc gtgctacgca ggaccttctc tttttctgac
     6181 gggttgacca tgttcgctcc tatagaaatg tgagaaaagg ttgttttata gtttatgtga
     6241 tttttgcttt aattcagtca tttattgcga gtttctttcg gatattccag aacatcatgg
     6301 aatgcaagat cctgatcagt tagtgacggt aaaattcagt aattaatcct agcgaatatt
     6361 cattgatatc acctgattgt gcgtaatatt ctttatacca ctcaactgtt ctccgtatgc
     6421 cgccggtgat gtcgtaggat gggtcccatc ccaacctctc ctttgacttt gaaatatcga
     6481 gcagaaggta ggctgcctcg tgggggcgtc gttcctggtg tgcatccagc tcccatgtgc
     6541 cgcttcccca ctcctttaat aggttttcaa cgagggtttg aacgtcgaca gcgttcccat
     6601 ggtgaggacc gaaattccat gcctctgcat attcacgtgg cttctcctgc attttcaatc
     6661 cgagcaggag gtatccgtag aggggatcca gaacgtattg ccagggacgg acagcatgag
     6721 ggtttcgcag aaccaccggt tcccctttca caagggccct gatgcaatcg gggacaattc
     6781 tgtccggagc ccagtcccct ccgccgatga tgttccccgc tctcgcggag gaaagggaga
     6841 ccagcggctc tccttttcca ttgtggaaaa aacttttacg gtaagatgat atcgcaattt
     6901 cagcggcacc cttgcttgcg ctgtatgggt cgtgtccccc gagcggatca gtctcttgat
     6961 aggcgtgcac taccccgcgg ttttcatagc atttgtcact cgtgatgcag acacaggttg
     7021 ttggaaatcg gagtgttcgt atggattcaa gaacatttac ggttccaatc acgtttatgg
     7081 cgaatgtctc aaggggattt tggtaagaaa ggcggaccag cggttgggcg gcgaggtgaa
     7141 agatatattc cgggcggtat ttttttatct ggtccaataa cgtattccgg tcctgaatat
     7201 cacctcttat atcaatgatc ctgtctccca attgcagaac gtcgaacaag gagggtattg
     7261 tggggggatc gagactgtaa ccgattaccc gggcgcccag cgattccagc cataatgaca
     7321 accaggctcc cttgaacccg gtatgtccgg ttataaagac cgttttatcc tggtaagcat
     7381 cagccagttc tttgtatgca gacattaatc ccacaatttc cagggcgctt ttccagttgc
     7441 ccagatccct tcgagataat tcttgtccct cagggtatcc atcgggtacc agaatccttc
     7501 gtgtttgaat gccccaagct gccttttctt tgccaaagac tccaggggct ccctttcgag
     7561 aatcgtagca tcattctcaa gataatcgaa aatatcaggt tcgaacacaa aaaaaccacc
     7621 attcacgtag tccccgtcac ctctgggttt ttcggcgaat gaaatgacgg tattttcccg
     7681 gatatccaac gccccgaatc tgcctgcggg ccggattgcc gtgacagttc cgattttccc
     7741 gttcttttca tggaatgata atagttcatg tatattgaga tcggatactc cgtctccgta
     7801 cgtaagcatg aatcggttat tccctatgta ttgctggatt cgttttaccc gcccacccgt
     7861 catggtgttg tcgcccgtgt caattaacga gactttccat ggttcggaat aattgtgatg
     7921 gatctccgtg gtattgcggt tcagatcgat cgtcacgtcc gacatgtgca ggtaataatt
     7981 tgcaaaatac tcctttatca tgtaaccttt gtaaccgaga cagataataa agtcgttaaa
     8041 accaaaatag gaataaattt tcatgatatg ccataagatt ggtttgcccc caatctccac
     8101 cattggtttg ggtctgacaa cagtctcttc tatgagcctg gtccccattc cccccgcgag
     8161 aattactact ttcgtgtgaa ccacccggat attatgttat ttttttagta ttcgtgcaac
     8221 agtgtaaaaa atgctattct tatctggacc ggacccatgg ttgagtatat agggcactct
     8281 ggcaagtaat ctttcgcata agaaggtatt tagtgaattg ttcaaaatat tgtgtaatag
     8341 ccggtttata tgacgaagat cgggttttac aattattata atatatataa caataaccgg
     8401 atgttcaatc ctgaaatcca ggccccgctc ggggaagatc ttttgtaccc aatgcattat
     8461 ctggcacggg ttgcaaagga aaaagggata tccgtttcta cgatcgatac cgaacccctg
     8521 gactcatatg atgcaatttt cttcatagat tttcctggtt acgggaacag gtatttcaga
     8581 aaattgttat caatgaaatt tgaaaatatg tacctttttc tctgtgaaaa tgagaatgtg
     8641 aaacctgata actggagaaa ggagaattac cggcacttca agaaggtttt ttcgtggaac
     8701 gaccgcctgg ttgataataa aaaaatattc aaatttttcc tggcaaacaa gatcccgacc
     8761 ccgttctcat tcgacgtgtc ggaaaagacg aaattttgtt gcatgattgc agcaaataag
     8821 tggaatgacc tgcctggcga gctgtattct gaaagggtac gggcagtaag gtggtttgaa
     8881 cagtaccatc cggaatcttt tgatctttac ggaaaagggt gggattttca gacaccgtca
     8941 tttttcaatc cccttcgacc gtatctcaaa cagatccgca attttttccc tccgaaatac
     9001 ccgtcctatc gtggagaggt tgcatccaag cgggcaatac taagtcaata caaattttct
     9061 atctgctatg agaatatgcg cgacgttcct ggctatatca ccgaaaagat attcgattgc
     9121 ttttttgctg gttgcgtgcc catttacctc ggggcgtcga acgtcactga atatattcct
     9181 gaatatacgt ttatagacaa gaggaaattt tcagattacc ccgaattata tgaattttta
     9241 atcaaccttt ccgacagtgc gtatcgtaaa tatctgcact ccattgaaga atacatccaa
     9301 agcgataaaa tttcgccgtt cggggctgaa tacttcgtga ataccattct aaatgaggtg
     9361 atctcaagtg catgacataa aaagtgaaat aggaataaag gacgaaaata tgttcaattt
     9421 ttattgatcc cctgtaactt gcgcaattga cgtaatatcg ttgccccaac aagatcataa
     9481 caaatgaagt atgtgatttt tgaatagggc aattccatat ggagaaagag ttggacatat
     9541 ttaatccata tgaattttgg ctttttctca aacggtgcac ggttcgttcg tacagcgaat
     9601 attggtgcgc ttgtaacaca taccattgcg cctttcttca aggaatcgat aatggcgtac
     9661 atatgaccaa aattagtttt tttgcgattt cttgcaattt tagggttaaa aatttccttc
     9721 ttaaatatat tacaggttat caggctgtgg ttcaatatag tccgcggctt atttctcccc
     9781 agaaaatctg tatattttcc acaccagacc cgttcaggtc cctgaccaat cccagaaata
     9841 agcaaggata cccgggatga tttgagaatg tcgataatac tcgcgattgc accttcgcac
     9901 aatatctcgt cgtcgccgaa tatccagacg taatcgccat gcgaacattc gacacatcga
     9961 acaaaattct cgtcgccacc aacgtttgaa tcgttccggt taaaccgtag ttgtgcagag
    10021 tggtttgcga gttcttgtat gtatgaaaac gatccgtcag ttgtacaatt gtcactgatg
    10081 aacagttcga tatctgggta tggttgactt tgtcgtaata attcaggtaa caattcttcg
    10141 agatattcac gacgattata cgtaggaact gctattgtga gaaaagtcat tgttcatttc
    10201 ctgatgaggc acacctgatt ccatgtagca tttctactaa tcttcactgt ttgactcaaa
    10261 ctgttcggca atctatgtgt atggaaagca ccaatcctgt gtttattaac atttgttttg
    10321 gaaataatgg ataataaagg gaattattct gtcccgaaca ggcattcacc catgtgtaga
    10381 aaaaagaagg agaattaaaa ggctatccca cattttcact gtgataattc ccttgcaaga
    10441 accgactccc accattgttg gatcataaca acccttatac aatccggtaa tccataattg
    10501 ttccaacata ccgagtttga tgccatatga ggttgccgga actgaaaatc accaggaagt
    10561 gccttttcat ccttgtcatc ctttttattc tgacattatt tacgctctgg atccgggttc
    10621 ttcccctcct caccctgaat accgatatcc tcaacattgt cgccgccgac gacccgatgt
    10681 acagcctccg ccagatcgag cagatgcttg cgaattatcc tgcgtatact tggtacgaag
    10741 cgatgtcgct gtatcccttc gggcaggaca tcccgtgggg cccgctgttc acgtggatca
    10801 cgacgaccat tgtcatgata tgcggtgccg caaccaggga cgagattatt tccgttgccc
    10861 tgtggatccc cccgctgatg gcggccgtga tggttccagt catgttcgtc ctagtccgca
    10921 agctcaccga ttggaaggcg ggcctctgcg ccgccgttct tattgcagtt gtcggggggc
    10981 agtatttttt cagggcactt gcagggtacc tggatcacca tgttgcggaa gtattgttca
    11041 gcacgatctt ctgcatcgcc tatatctatg cattggccta tttcagggag caagcagtcg
    11101 accttaaaaa taaggagaca tggaaggttc cggtatttct ttccgtcatt gctggaatcg
    11161 ggtacttcct cggctatctc accatgtcga caatgatcct ttttgcgttc atcgccgcgc
    11221 tctctaccat tttcataagt gttgtcgact tttatcatgg caagtcgggg gagtatatgg
    11281 tgctgctcaa taccgtgtca ttcggccttg cagccgtcct gtctcttttg gtaagtatca
    11341 tttccggcgg ttttgacgaa gggttcagtt tcaatcttta tacacttctc cacccggttt
    11401 cgtatctgct gcttgtgatc gggacatggg tcctctattg gcttgcgcgg aatcttaaag
    11461 ggaaaagcca gatcctctat cctgtctcgg tcattggtct cggctttatt gcgtttttaa
    11521 gcctgtatct cttcattcct ggtctttatg gatctttcgt cagtggaatc gaggtgttct
    11581 tcggcctcaa cccttacgcg gttacggtac aggaggcgcg cccatggagc ctcgttgagg
    11641 catggcaggt attcaacttc ggcctcatcc tgatggcggc gggagctttc gtcctcctgt
    11701 acaggagctg gaaggaatac aggccccagt atatattcgt catggtctgg tctgtattca
    11761 ttattgtcgc cgcgttccga caggtcaggt acgaatatta tcttgccgtg aacatcgccc
    11821 tcctaggagg catcgcactt gggttcttcc tcgacctggc atggccagac atctgcgcaa
    11881 tggggagacg cgtgactgca aacgaggcac ggaaaggacc agccgaggaa acagcccttg
    11941 ccaaagaaga ggaacgaaaa cccgaactca agtcggagaa aggaaagggg aagaagtccg
    12001 ggaagaaaca ggaaccgtca cataaaactg ttgaaaaacc tgaaaaggct tcgaagaaag
    12061 ctgcaccgga aactcgcatc aggtacatcc atgtcctcgt cgcggcgctc ttctgcgcct
    12121 gtgccctcct ctttgcagtg ggatcggtcc aatacgagta tgcggtctca gcatcgggag
    12181 gtatccggat gaacccggac tggcgcgaat ctctggagtg gatggaggcg cacacccccg
    12241 acactggggt tgattacctg cgaatctacg atgagaacac cttcaagtat cccgatactg
    12301 cgtacggggt tatgtcctgg tgggactacg gccacctgat cacttatatc gcaaagagga
    12361 tcccgaacgc aaacccgttc cagtcaggag tcgcgggaga gaatggtgcc gcgacgttct
    12421 tcctctccga gaccgaggag aggaacaccg cgattgcgga tacactcggg accaggtacg
    12481 tgatgaccga catcgagatg tcgagcggga agttctgggc catggcgacc tggtacaact
    12541 cgagtgcggg ccaggcccca tacgtcaaga ccgtgcttgc gccggaggag ggtccccagg
    12601 gcacctacag cgcggtctcc ctctatgatg catcgtattt caacaccatg attgcccggc
    12661 tccacaactt cgacggctca atggttgcgg gaggccaggc ctactatgtc gaggtcgatg
    12721 acaggctgtc ttcctacccg gtggcgacgg ccgcgcagat gatggatacc gcggttgcaa
    12781 gggcagctgc cgatgcctac aaccagaagg ctgcaccagg gacccgtgcg atggtcctct
    12841 cctcaaacct cctgaccccc atcgacaccg tccctgcgct caaacattac cgcctggtgc
    12901 atgaatctcc caccaatgtc attccggcag gtgcaggttg ggatatcaag tacgtgaagg
    12961 tattcgagta tgtcccgggc gcacggatcc agggcacggg ggtcattgcc cttgacctgg
    13021 tgtccaacac ggggaggacg tttacctata agcaggccag caccgatggg gaattcatcg
    13081 tcccctactc gaccaccggc tctccctacg aggtaaaggc ggcaggcagg taccgcatcg
    13141 agggcaccgg ccgggagatc gacgtcccgg aaactgcggt catgcagggg ctgcaggtcg
    13201 ggtgaatggc atcccagtgc tccgtcattt ttggagagga gttcacccgt cacgacctgg
    13261 gctggcacga tgagaacagc cggcgcctga cccgggcgct tgagtgcatc ccgaagaact
    13321 acaaggtaag gccggtggaa gaggcgtcgt acgatgacct tgcccgcgtg caccatcccc
    13381 ggcacatccg gatgatcgag gagctgtgcg ggtacggggg acggcgctac ctggacatgg
    13441 acacctatat caccgaggac tcttaccagg tcgccctgta tgccgccgga tcggcgattg
    13501 ccgccacgcg cctcgcgctg gagggcgaac actgctttgc cctgatacgt ccaccgggtc
    13561 accatgccga acctgacaag gccatggggt tctgcctctt caacaacgcg gcagtggcgg
    13621 cagcctgggc gctcgagcgc gtcgaccggg tcgcaatcgt ggactgggac ctccatcacg
    13681 gaaacggcac ccagaagata ttttactcaa gcgacagggt tctcttctgt tccatccacc
    13741 agcagaacgt gtttccctgg agcgggtgga tagacgagat cggcgaggga aggggccggg
    13801 gcttcaacct gaacgcccct cttcccgccg gcggagggat cggcgactat gctctcatct
    13861 tctcagaggt attctcagac gccctctcgc atttccaccc ggacgtcgtg atcgtctccg
    13921 caggccagga tcccctggaa gacgacccaa aggggggtat gaggctcacc ccggaagatt
    13981 tcgggctcct ggcagctctt atcagggatg catgccacca gccgctggca ctgctcctgg
    14041 agggcggata tggtccctcc ctcggcgatg cgatcgcatg catccttgcc gtgctgaatg
    14101 aagggaggac tccggaaatc ccggacgtgc cggaaaggga gagcaccagg aggactgccc
    14161 ggctcctcag gagagtgatg atctgaatgg tccctgggcg ccgctaaccc tgctgcctta
    14221 ccttccggtc cttgtggagg tgcgagacct cgaggccggg gaccaccacc cggacgaccg
    14281 ggaccgctgt cctggggagg tcgaccacgc acacccgctc aacgcctgga cgcaaatcgt
    14341 caatgatgag cgatatgtct tcgtcaatca actgtgagct cacatcaggc agagacgaga
    14401 cgggtatttc cggggcatcg gcaaaccact cccggttaat ccgtttcatt cgttcgtacc
    14461 ccgccctcga gaggagcatc tcccggtggg aagaggtggt cccttcgcga agatacgcgg
    14521 cacggctgaa cgccacctcg gtcagtgccc tggctgccgc gatctcggga tcggtgtggc
    14581 accctgaacc catcatcagg agcgcaggat ctctcgtgac ctggtcgtct gcagcagcgg
    14641 caaccaccgg gacccgtgtc ctcccttcga ggagccagag gtgaattgcc acgcctgccg
    14701 aggtgaaccg gtccaggagc gcccgtccag gcccacccgt atccaagacg agcctctttc
    14761 ccatgtccct cttcctctcc gcacggctca tcgcgtcccg ttcgaggagc tccaggagcc
    14821 cgtgaaggat ggcctcctcg cgcacgttcc cgctcgcaag gcccgacggg tcgctctgga
    14881 agaggggcat ggtcataccg agcgtgtcgt agggaaagaa aacggcgttg ctcgggacca
    14941 tgacctgttc gcagttcatg atgtctgcgc caggtgtcca gtgcagcttt tcgccttgct
    15001 cgagcggcct cgggaggatc agcgactcgg ggtcgactgc cctggcaacc cccgcctcct
    15061 cgtagctggc atacaccatc tggtccccgt gatattctcc cgagaacctc tcgattgccg
    15121 ccatcatcgc agagaccctt gcgaggggaa ggtccagccc cataccgggg tggacccctg
    15181 tccccccccg cgggatgcgg ctgcgggagg agacgcagca cgggatcccc acgcggtcac
    15241 tcctggtgat gtccctgacc gggccggccc cgatggactt catcaggggg cgggccatct
    15301 tttcggtctc tttgggtgta cacgtgcggt gggtcccatg gaaatagcgt ttctccaccc
    15361 ggttcagttc gcattccatc actaccccgt tcgccgggaa gagttttata gtttccaagc
    15421 tcgggagagt gtgtatgcag gctgatgaag tagaggtagg gatgcgggtt cgctatccga
    15481 ggacagggac cacgggccgg gtcgttgccc tggagaacgg gatgggcgga ttattcgcgg
    15541 aactggactc gacccacctg cgttaccggg tcgaccagct ggtccctgca ggtgtcgcgg
    15601 gtgaacgtgc caggaccaca aaagaagacc tgatggaccg gctgaaaaag gaacgggaat
    15661 tcctctcagc gacggggttc caggacgcga tatcccacac ggaccagagt tgcgagggcg
    15721 ggggctagtc ggttatcggg atggtggtga gtttcaccga ctctacccct ttctgggaca
    15781 tgaggcgctc agccagggtc ttgagttctg atccatcgcc cctgaccagc accacttcca
    15841 ggcacttgtc gtgggtcacg tgggagtgga gcgatgcctg gatgatgccc atgaactcgt
    15901 gctggatatc ggtgagcgtc gccaggaggt ttctctgttc gtggtcgtag atcatggtta
    15961 tcactccctg gcgctcccct ttcacgtccg acatccattt ataataggtg atgtagttgc
    16021 gtatggcatc ccggatgccc tcggaacggg atgaataccc gcggtaattg atgatctcgt
    16081 cgaatttatc gaggaggttc ttcgggagcg agattccaat cctcgagagt tcagtatcta
    16141 tggtcatggt tgtgatcctc tccacatggg atctcttata tataagtact taaaacttcg
    16201 gaaaatcacc cctttccagt gctacttagt aatactggct ggggcatatt tcctgtgcaa
    16261 gtgccggcat tcggtacttt tatctggttt tcgctccgag tgtgttgaag agagcatgtc
    16321 agccccttcg taccacgccc attcggagaa aggcctgcgg gagaggaacg aggacgcctg
    16381 catggcgagg gaggtgggcg gcatacatct ctttgccgtt gcggaaggaa tgggcggatc
    16441 cccggaagga cccagtgcgg ccgaggtggc catcagcgcc ctctgcagcg gcgaaagcgt
    16501 atatgccggt tcgctggcag acatggcgga atccttcctg gcccgtgcag acgatgcgct
    16561 cttccactcg cacgagtcaa accccgtggc tgatccccct gcggtggcca tgacgcttgc
    16621 aatcgtggga ccaggcggtg agtgcctggt gagcagccct ggccagcgca aggtcttttt
    16681 catgccggtc ccaccaggag agaacaaagg accggatccc aacgatattc aggactcacc
    16741 cgttctccat gatcccggtg ccgggatgca cgaggcagta cttcctcccg ggtttctgat
    16801 cctctgctcg gacggcatca gcgagttcgt tacagacgac cggatctccg agatcgtgag
    16861 ggagagaggc gatgacctgg agggtgcctg ccgcaagctg gtgtacgaag cgttccagaa
    16921 tgggagcaat gacaacctta ccatggtgct ggtcaggcaa cctgaccggt gaggccgctc
    16981 ttcacctgag tggagatccg gccatgaacg attattcgcg ctcttttacc tgccgctggg
    17041 ggaatcgtgg atagcatcac ccacgggctc gttgcgatgc tggcgttcca cgcgacgttt
    17101 ccgggcttgc tggtactctt tgcggtgctg ggggcagttc tccccgattc agatgtcttt
    17161 ctgtcgctcc tctctgaccg tgacccgcgc ctctttgtct tcacccatgg gggattcacc
    17221 cacagtattc ccgggtccgc agccattgcc ttcggtgtcg cggctgggtt ccaggcatgg
    17281 ctgtgcctgg caggccaggg gcatgtgggg ggtacgttcc tggtcttggc ctgggcgctc
    17341 gcatgggcgg gtgcgctctc gcacattgcc ctcgatgcgc tcgcgtttcc cggcatcccc
    17401 ctcctgtatc ccgccagcac caggaagtat tctgccggta tattcccggg cccgagcctt
    17461 gtgctgtttg gaacgagcct gggcttcctg ctgatgcact tcgccggata cgccggcacc
    17521 ggcgcactct atgcctacat ggcttttatc ggggtgttcg tcgcggcgca tgtcattctg
    17581 aaggcctttg ttgcggcacg tcatccggga ctcaccatcc ccaccttcaa ccccatgaag
    17641 tggctggttg tcagggaggt ggaggactca tacgaggtat gctcggccgg actttcacgg
    17701 gagtgcacga gcatcgcggt atttccaagg tacgatggag tcaggccgga gtacctcgac
    17761 cggcattcca atgacccgga gatggcaagg gttacttatc actcgtatat caccgtggct
    17821 gcccgaagag acgggtcgat cgtcgtgcgt gaccctctgc gggattcggg catactccgc
    17881 tatcctccat actaccggag cgtgcgcctc ccggaagagg aaaggggcac cccctgatcc
    17941 cgtcttcttt tgggcacatc ccggcaggag ctgcgccccg gccactctgc agaccggaga
    18001 aaaaccacac cttcgcgaaa aaatccctgc tcgcagggta ttaagtagga ttgttcttat
    18061 atagatcagc gaggtttgat c
//
LOCUS       DAOQYR010000041        18018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_3505_length_18018_cov_8.608640, whole genome shotgun sequence.
ACCESSION   DAOQYR010000041 DAOQYR010000000
VERSION     DAOQYR010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 18018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18018
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3505_length_18018_cov_8.608640"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..1152
                     /locus_tag="PKM87_06335"
     CDS             4..1152
                     /locus_tag="PKM87_06335"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNQ29721.1"
                     /translation="MYAERMEMLPPYLFARIDEMKAKKIQAGVDVIDLGVGDPDLPTP
                     PFVVEELCRAARDPAHHHYPSYAGMTDYRRAVAGWYTRRFGVELDPSTEVLALMGSKD
                     GIAHVPEAFVNPGDYVLAPSPGYPVYRTSTLFAEGKVYEMPLLAKNHFIPDLDDIPNK
                     VADAAKIMFLNYPNNPTSAVTLPGFFESAVEFAREHEIVIIHDNAYSEISFDGYRAPS
                     FLETEGAMDVAIEMHSLSKTFNMTGWRIGMAVGNREILAGLGRVKTNVDSGVFDAIQR
                     AAIAALSGPDDCVRDACRVYQERRDVLVGGLRRLGFSVETPRATFYVWMAVDDCMAFA
                     TRMLDEAGIVVTPGIGFGSGGDGYVRFALTRSTDRIRQALERMEAMDL"
     gene            1149..2441
                     /gene="lysA"
                     /locus_tag="PKM87_06340"
     CDS             1149..2441
                     /gene="lysA"
                     /locus_tag="PKM87_06340"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNQ29722.1"
                     /translation="MKLPPHLGIQDSRLTIGRLDCVSLADRFGTPLYVTDQDRIVDQF
                     RRYRSALASHYPEVQVLYAAKANGNLAILSALAREGAGADVFSSGELYLALDAGIPHG
                     RLLFNGSSKSRADLKLAVEKDVPVSVDSLDELHQLDEVAGSLGRTARIAFRVNPALDV
                     PTHPKIATGLATSKFGIAHHLIPSAYREALGCTHIRPCGLHCHIGSQILDLEPFRKAA
                     EVMMRIASDLVAMGIDLEFIDLGGGLGIPYRHGTDMAPTPEEYATVVVPAVARGVEAL
                     GITPELWIEPGRFLVADSTILLTRVNSVKKAHKNFVNVDAGFNLLVRPAMYDSYHEIV
                     AAQSADRIPEETYTITGPICESGDILASDRRLPLVRAGDLIAILDTGAYGFAMSSQYN
                     SRPRCAEVIIHDGAPALMRRAETIEDIKATMRQPPWQS"
     gene            2457..3794
                     /locus_tag="PKM87_06345"
     CDS             2457..3794
                     /locus_tag="PKM87_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29723.1"
                     /translation="MQFHYALVDDLFSREEFERRVEEKMESCGDLVDEVTAAMMVVQD
                     CGRHHVKIKDLSGRSSLFSFFGKVIAKNPPREFERPGGEKGLVTSLILGDETGQVRAI
                     LWDEKAMAAQEIEVGEVLEVIGRHPNRTPTEITVMAMRQAMVEIVGPTQVEPGLAPPK
                     RKEITARVMAVGEPRMVPRRDGTETEVLDLVVRSIEGIFRISCWVPDLIEGIEEGATL
                     RVQGVLEKVRKYGKEYSIDERSIIEIADEDIPQEIDSISDVKEGGIFSVCGSVRSCQP
                     ARSFTTRDGRPSCVRNLLLTDGSHEIRVVFWGEEHARTELLPEDRVTLYLCSARQGRS
                     GELELHAGSGSMVKVHHEGEAGEGEITGTVLATSGGTFLDTGAEHFLVRGHLPHGHEV
                     RARVTRRGKRISINSFEEVLPDLEELNARIDAFLAMTEAGNTFTPHGRQDISK"
     gene            3828..4799
                     /gene="radA"
                     /locus_tag="PKM87_06350"
     CDS             3828..4799
                     /gene="radA"
                     /locus_tag="PKM87_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadA"
                     /protein_id="HNQ29724.1"
                     /translation="MSNGPIELEDLPGVGPTTAEKLREAGFLSVENIATASPQDLAET
                     AEIAESTAKKMIKAAREMVDLAGFRTGKDVFEQRKEVRKLKTRVPELDALLGGGLETQ
                     AITELYGEFGSGKSQVAHQAAVNVQLSEDEGGLNGSAIFIDTENTFRPERIEQMVIGL
                     GIDADPQEFLENIHVARAHTSDHQMLMIESAKEKAQELKNSDRPLRLIIVDSLTAHFR
                     AEYAGRGTLAARQQKLNRHLHDIFRLVDDHNAVGMVTNQVLSNPAVFFGDPTKPIGGN
                     IVGHTATFRIYLRKSKGGKRIARLVDSPNLPEGEAAFMVEEAGLKPV"
     gene            5374..6087
                     /locus_tag="PKM87_06355"
     CDS             5374..6087
                     /locus_tag="PKM87_06355"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HNQ29725.1"
                     /translation="MNRSFCLKGLVTDIDGTITDPRRRIHTGAIEILRDLMEKGVYVV
                     LASGNTSCFMDAVSKMIGTPGTYIGENGGIIRNGYGQDITLLGDGAAPRKALCDLVEA
                     YQRKGISIEHYSLPYRFVDVAFARTLPVDEVRGILEHHPVEVLDTGFAIHIHPPGVNK
                     GVAFSELARAMGLDTRDFLAVGDAENDIELLRRAGIGVAVANAHPQLVLYADHVTERE
                     CGDGFIEGVKKYMAYFLER"
     gene            complement(6069..6320)
                     /locus_tag="PKM87_06360"
     CDS             complement(6069..6320)
                     /locus_tag="PKM87_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNQ29726.1"
                     /translation="MSRVLARSLSKKKIVTNEGKVIGTLKNLVVDFETGQVVDLVIYP
                     DPSFDTSGYRTEGDRLYIPFEAVKDIKDYIVVDRYLSRK"
     gene            complement(6452..7330)
                     /locus_tag="PKM87_06365"
     CDS             complement(6452..7330)
                     /locus_tag="PKM87_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNQ29727.1"
                     /translation="MDLSLIQKDILITLITLYHQHSHPIKGEEIAEVIKRNPGTVRNQ
                     MQALKAIGFVDGIPGPKGGYNPTAQAYRALNLDISGNEYDVKIARNGEEVQGVKVVEI
                     AFTTLCHPDICRANATLIGSVKAFGIGDQITIGPTPVNKLLIRGEVYGKDEVSQTLLI
                     SIFEMISLPKKPIRYYMSSPLISLCRADPLKNALHLFNSHHIRGAPVVEKDELFGIVT
                     MSDVANAIEKNVPMDTPVSTIMTPDVVFAPSSVQLFEVIRSFKERDIGRLIVMEEGRP
                     VGILTQSDIIKVFPSL"
     gene            7378..8334
                     /locus_tag="PKM87_06370"
     CDS             7378..8334
                     /locus_tag="PKM87_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNQ29728.1"
                     /translation="MEFETIPTVPTADEILDRSFRRAASKMKLKKNKDRANEEFVRAV
                     SQAIHDRLVKVIQSFPDFDAVSPFYRDIVDIMWGLDRVRKALGGVGWAARWARTHGPG
                     LAYQTRKSENPSQIRKRAVARLSSVVHQVEEDLLFLNEVRNVVRKLPHVSDEFTVVVA
                     GYPNVGKSSFIRLVSTAVPEVASYPFTTKGIIVGHRAVGKERMQFIDTPGVLDRPSDE
                     RSGIERQALTAMVNLASVILVIIDASEHCGYPLEDQFRLLHEIEEMVSVPVKAVVNKS
                     DLAYFDGYINMSTETGEGVDEVLESLLAYRKRSEGESQAVPG"
     gene            complement(8302..9189)
                     /locus_tag="PKM87_06375"
     CDS             complement(8302..9189)
                     /locus_tag="PKM87_06375"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042500 - aspartic endopeptidase activity,
                     intramembrane cleaving [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presenilin family intramembrane aspartyl
                     protease PSH"
                     /protein_id="HNQ29729.1"
                     /translation="MNLSRHIPIIAMPAMLVLVEIGAVLLSLPMQQAGLAAFEDPASV
                     ANPLIFIGILLVFTAFLLVLIKYKFRRFIGAIIAVSIFFTLIYIFGSITYALTDDSLA
                     GLVGSFLPAIVATLLLYLYPEWYVIDTLGLLIAAGAASIFGISLEILPVIILLILLAA
                     YDAISVYKTKHMIALAEGVVETKSPILFVVPKKRGYSYRKEGLGPLEEGERGAFIIGM
                     GDMIMPAILVVSANVFFQGAKLGFLTIPALGAMAGSVAGLSVLLVYVNRGKPQAGLPP
                     INGGAILGFLAGYGLALAL"
     gene            9246..9560
                     /locus_tag="PKM87_06380"
     CDS             9246..9560
                     /locus_tag="PKM87_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29730.1"
                     /translation="MDIREYLGDLLLLLILVISTLILVFNLNFDLIKSFAAALMMLSL
                     GGLIFLVHWKVRRIEKNLANRERMVRMNMEEIAAKMAQKYDTHVSHIDSVVEEFAKRI
                     YR"
     gene            9589..10593
                     /gene="fen"
                     /locus_tag="PKM87_06385"
     CDS             9589..10593
                     /gene="fen"
                     /locus_tag="PKM87_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease-1"
                     /protein_id="HNQ29731.1"
                     /translation="MGVAFRDILTEYKDPVGWETLPGVAAVDAHNALYQFLSIIRQPD
                     GTPLMDQEGRITSHLSGILFRTANFLEKGIRTVWVFDGTPPDFKQQTIEVRRAIRDQA
                     GEKWQDALARGDIAEAYKQARSSSRIDEDVIKTSRELLTLLGLPCVQAPSEGEAQAAH
                     LVGKGDARYVVSQDYDTLLFGAPVLVRNLTVSGKRKVRGRTISVFPEKILLSEVLKGL
                     SLTREDLVRIAILIGTDFNSGIRGVGAKTALKMVQKGEFERTMREKLPNTDWEPIMEF
                     FMNPPVTENYSLQWNSPDGEGIVRMLCDRYDFSPERIQKALDGMGAKSGQKTLDSWFS
                     "
     gene            complement(10699..11295)
                     /locus_tag="PKM87_06390"
     CDS             complement(10699..11295)
                     /locus_tag="PKM87_06390"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNQ29732.1"
                     /translation="MTLEILRPHMKEIMAPFISVSKKIGLSPNNLTIGALITAAAAGV
                     FFYLGNVLAGTAFVAFNALLDGLDGALARAENIQGPKGDFLDHVVDRYADIFIITGIF
                     AGGFASWQVGVLALTGVLMSSYLGTQAQAVGVGRYYGGVLGRADRLVLILCAGVLTVF
                     VPDTFFGLSILGWLLLLFGVMGHVTAAQRFFYVWRKMP"
     gene            complement(11292..11804)
                     /locus_tag="PKM87_06395"
     CDS             complement(11292..11804)
                     /locus_tag="PKM87_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase family protein"
                     /protein_id="HNQ29733.1"
                     /translation="MMIGITGTPGTGKSTVAQELKARGYRVTSLSTTTTPYILARDEA
                     RHTLVIDEEAWASEFQKVEGFVEGHLAHLLPCDLIVVLRCRPDVLAARLRARGYPEAK
                     VSENVEAEAIDVSLIETLEEFSGDQVLEIDTTERDIGECAGLIEEFARGKVPPSFGKI
                     DWSAYLGEPK"
     gene            complement(11801..12880)
                     /gene="hisC"
                     /locus_tag="PKM87_06400"
     CDS             complement(11801..12880)
                     /gene="hisC"
                     /locus_tag="PKM87_06400"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNQ29734.1"
                     /translation="MAKENDSRYTSLVRDCYRSGGYVFARRAGNTGEDDEACRVARLA
                     SNENPRPPSPLALERGCQALREVNRYPDERMADLVRALAKFHGQYHFVTGVGMDGVIE
                     TVIRTLVCPGERVAVSTPTFSFYGLAAMAQGAEVVNSPRRPDFSVDVHQFVRSARDAK
                     ISFLCTPNNPTGNVTSRDDVEEILSAIHGVLFLDNAYVEFCDEDYRPLMNRYENLILG
                     RTFSKVFSLAGLRVGYAFVPLWLVPCYLRAATPFTVNSVSAAAATGALADRLHLKETV
                     EGVKRLRELVVRECKYPVFHSGANFVMVDVSPSSGREMADRLAERGVLVRSCESFPGL
                     GPHYIRVSIGEEWENARFLDVINAL"
     gene            13078..13695
                     /locus_tag="PKM87_06405"
     CDS             13078..13695
                     /locus_tag="PKM87_06405"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNQ29735.1"
                     /translation="MEQNRLYTLPALPYDYKALSPHISEEQLRIHHTKHHQGYVNGAN
                     AIYEKLDKARREGSDLDVKAVHKELSFHIGGFLLHALYWENMAPAGKGGGAPKGPLSA
                     HIEKDFGSFERFKKEFTTAANSVEGSGWAALTFCQKTGRLLLMQIEKHNVNVYPAFPI
                     LMAIDVWEHAYYVDYKNDRAKYIDNFWGIVNWDTVNARLEKVLKK"
     gene            complement(13708..14838)
                     /locus_tag="PKM87_06410"
     CDS             complement(13708..14838)
                     /locus_tag="PKM87_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HNQ29736.1"
                     /translation="MQNKEDYRAIEARYYMPAFARDLMIVRGEGSRVWDADGKEYIDC
                     VAGIAVCSTGHCHPAVTRAICEQAKRLIHCSNLYYVPGQAELAQKLVEKTGMAKAFFS
                     NSGAEATDGALKLARVRTGRKKFVAFTHGFHGRTIGSLAVTHKPAIREPFEPLGISKT
                     FVEYGDIDGLRKVVDSDTAGVVVEPIQGEAGVIIPPDSFLHGIREICDESGALMIMDE
                     VQTGMGRTGKWLAIQHAGVKPDIVMLAKGIASGFPMGALLAREGLEFRRGEHGSTFAG
                     GPLACAAALATFGVIDEILPTVSEKGERFCNALSKYAPRARGLMVGMTVGDRCAEVQK
                     RCAENGVLVNCAADGNLRLVPPLVITNAEIDRACSVLKDALG"
     gene            complement(14867..15784)
                     /gene="guaA"
                     /locus_tag="PKM87_06415"
     CDS             complement(14867..15784)
                     /gene="guaA"
                     /locus_tag="PKM87_06415"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNQ29737.1"
                     /translation="MINAEKFISNAVEEIRREAGERRVVMALSGGVDSSVCAALASRA
                     IGDRLEPIYVDTGLMRKGETERISQVFGKLHLHVVPAEEEFLGALRGVRDPEEKRKVI
                     GERFIRVFEREARRSGARALLQGTIYPDRIESEGGIKSHHNVGGMPLHMEFETVIEPL
                     RDLYKDEVRVLAGALGLPHEIQHRMPFPGPGLAVRVIGEVTRENIEVVRQANWIVERE
                     IVEQFNPWQCFAALLGLGTGVKGDNRVHGWVVAVRAVNSRDGMTADPVQIPWDVLDRI
                     ASRITSEIPSVARVVYDITPKPPATIEYE"
     gene            complement(15798..17384)
                     /locus_tag="PKM87_06420"
     CDS             complement(15798..17384)
                     /locus_tag="PKM87_06420"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNQ29738.1"
                     /translation="MKYIFITGGVMSGLGKGITAASVGRILKNRGYGITAVKIDPYLN
                     IDAGTMNPGQHGEVFVLSDGSEVDLDLGNYERFLDIDLSSAHNITTGKVYRTVIEKER
                     RGDFLGETVQIIPHITDEIKACIRAAADMEIPGQKKADICLVEIGGTVGDIESMPFLE
                     AVRQMRGDLTLQDTVIIHVTLIPEDTMGDLKTKPTQHSVKALRELGLHADIIVGRSER
                     PIGSHTKRKISAFCDLPLKGVISAATVPDIYQVPLELEKEGLGDVLSEHLGLEKRRAD
                     TSWYHLVSREYTDRVSVGIVSKYGIEDVYLSIKEALKHAGRALSTEVRITWLDAERYE
                     QKQLAEFDGILVPGGFGKRGIEGKVDAIQWARTNRTPFLGLCLGFQMAVVEYSRHVLG
                     WEDATSAEIGEGTHVIAILPEQHEVKELGGTMRLGDSPITLKEGTLAMQVYGQSAITE
                     RHRHRYEVNPRFIPDLEKAGLVFSGLFQNRMEICEIPDHPFFMATQFHPEFKSRPTRP
                     SPPFVGFVKACSSRNGRRAR"
     gene            complement(17589..18011)
                     /locus_tag="PKM87_06425"
     CDS             complement(17589..18011)
                     /locus_tag="PKM87_06425"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNQ29739.1"
                     /translation="MNYLARIREMGSKANPFFGLMGIEVEHAGEGEAIISMTVRPDML
                     NGEGWLQGGVFTALADEAMVLAIYSLIGPDDRIATITESTTFIGGSREGCLYALGKVV
                     KKGRRVVFAEGEVRDGARAGPLLSRSTAAFAVRAGHRT"
BASE COUNT         3887 a         5398 c         5043 g         3690 t
ORIGIN      
        1 cacatgtacg cggaacgaat ggagatgctc cctccctacc tgttcgccag gatagacgag
       61 atgaaggcaa aaaagataca ggcgggagtc gatgtcattg acctgggcgt gggtgacccg
      121 gacctgccca cccccccttt cgtcgtggaa gagctctgcc gggcggcaag ggacccggct
      181 caccaccatt acccgtccta tgcagggatg acagattacc gccgcgctgt cgcgggatgg
      241 tacacacgtc gtttcggcgt ggaactggac ccttccactg aagtgcttgc cctgatgggc
      301 tcaaaggacg ggatcgcaca tgtaccggag gcattcgtaa acccgggaga ttatgtcctc
      361 gcaccgagcc cgggttaccc ggtataccgg acatccaccc tctttgccga gggaaaagtc
      421 tacgagatgc cacttctggc caagaaccac ttcatccctg atctcgatga catccccaac
      481 aaggttgcag atgccgcaaa gatcatgttc ctcaactacc cgaacaatcc cacatctgcg
      541 gtgaccctcc cgggattctt tgagagcgcg gttgaatttg cacgcgaaca cgagatagtc
      601 atcatccatg acaacgccta ctcggagatc tccttcgacg gctaccgggc tccctcgttt
      661 ctcgagacgg aaggcgcgat ggacgttgca atagagatgc attccctctc gaagaccttc
      721 aacatgacag ggtggcggat cggcatggcg gtgggaaaca gggagatact cgcagggctc
      781 gggagggtga agaccaacgt cgactccggg gtctttgacg caatacagag ggccgcgatc
      841 gccgccctct cgggcccgga cgactgtgtc cgtgatgcct gccgcgtcta ccaggagcgg
      901 cgcgacgtgc ttgtcggggg actccggcgc cttgggttct ctgtcgagac cccgcgtgcc
      961 accttctacg tctggatggc agtcgatgac tgcatggcct ttgccacacg gatgctcgac
     1021 gaggccggga tcgtggtcac tcccggcatt ggttttggat cgggcggcga cgggtatgta
     1081 aggttcgccc tcacccgttc cacggaccgc atcaggcagg cgctcgagag gatggaggcg
     1141 atggacctgt gaaactccct ccccacctgg gtatccagga cagtcgcctc accattgggc
     1201 ggcttgactg cgtctctctc gcagaccggt tcggtactcc cctctatgtc acggaccagg
     1261 accgcatcgt cgaccagttc cgccgttaca ggtcggcact cgcctcgcat tacccggaag
     1321 tgcaggtgct ttacgcggcg aaggcgaatg gaaaccttgc aatcctttcg gcccttgcgc
     1381 gggagggagc gggggccgac gtcttctcgt ccggagaact gtaccttgcg ctcgacgcag
     1441 gtattccgca tggtcggctc ctgttcaacg ggagttccaa gagcagggct gaccttaaac
     1501 ttgccgtgga aaaggatgtc cccgtctcag tggactccct cgatgagctt caccagctcg
     1561 acgaggttgc cgggagcctt ggcaggaccg cgagaattgc gttccgggtc aacccggccc
     1621 tcgacgtccc cacccacccg aagatcgcga ccggccttgc caccagcaag tttggaatag
     1681 cccaccacct catcccttca gcctacaggg aagcgcttgg gtgcacacac atccggccct
     1741 gcggcctcca ctgtcacatc ggttcccaga tactcgatct cgagccgttc agaaaggccg
     1801 cggaggtgat gatgcggatt gcctccgacc tggtggcgat ggggattgat ctcgagttca
     1861 tcgaccttgg aggggggctc gggatcccat accggcatgg gaccgacatg gcgccgaccc
     1921 ctgaagagta cgcgaccgtc gtggtcccgg cggtcgcaag aggggtcgag gcactcggga
     1981 tcaccccgga actctggata gagcccgggc gttttctcgt ggcagactcg accatcctcc
     2041 tgacgagggt gaactccgtc aagaaggccc acaagaactt cgtcaacgtg gatgccggtt
     2101 tcaacctcct cgtccgcccg gccatgtacg attcctacca cgagatcgtg gccgcacaga
     2161 gcgcagatcg catccccgaa gagacctaca ctatcacagg gcctatctgc gaatcgggcg
     2221 acatccttgc ctctgatcgc cgtcttcccc tggtccgtgc aggagatctc atcgccatcc
     2281 tggacactgg ggcatacggt tttgccatgt cctcgcagta caacagcagg cccaggtgcg
     2341 cggaggtcat catccatgac ggcgcgccgg ccctgatgcg cagggcagag acaatcgagg
     2401 acatcaaggc cacgatgcgg cagcctccat ggcagtcctg aaggggagag gggccggtgc
     2461 agttccacta cgctctcgtt gatgacctct tctcgaggga ggagttcgag cggcgggtgg
     2521 aggagaagat ggagtcctgc ggggacctcg tcgacgaggt gaccgcggcg atgatggtgg
     2581 tgcaggactg cgggcgccac cacgtgaaga taaaggacct ctccgggaga tccagcctct
     2641 tctctttctt tggaaaggtg attgcaaaga acccgccccg ggaattcgag cgccctggcg
     2701 gggagaaggg acttgtcaca agcctgatcc tcggggacga aacaggccag gtgagggcca
     2761 tcctctggga tgagaaggcc atggccgccc aggagatcga ggtgggagaa gtgctcgagg
     2821 tcatcggtcg ccaccccaac cggacgccga cggagatcac ggtcatggcc atgcgccagg
     2881 caatggtgga gatcgtgggg cccacccagg tcgagcccgg ccttgcccct ccaaagagga
     2941 aggagatcac ggcccgggtg atggcggtcg gggaaccccg gatggtacca cgaagggacg
     3001 gaaccgagac ggaagtgctg gacctcgtgg tccgcagcat tgagggcatc ttccgcatct
     3061 catgctgggt gcctgacctc atagagggga tcgaagaggg ggcgactctc cgggttcagg
     3121 gagtcctcga gaaagtgcgg aagtacggca aggagtacag catcgatgaa cggagcatca
     3181 tcgagatagc tgatgaggat atcccccagg agatcgactc tatctccgat gtgaaggagg
     3241 gcggcatatt ctcagtatgc gggtccgtcc gatcctgcca gccggcacga tcgtttacca
     3301 cccgggacgg gaggccctca tgcgtccgca acctcctgct cactgacggg tcgcacgaga
     3361 tcagggtcgt cttctgggga gaggagcatg cccgtaccga gctcctcccg gaagaccggg
     3421 tgacactcta tctctgttct gcccggcagg ggagatcagg agaactggag cttcatgccg
     3481 gcagcggaag catggtaaag gtgcaccacg agggcgaggc cggggaagga gagatcaccg
     3541 gaacggtcct ggcgacttcg ggagggacct ttcttgacac gggtgcagag catttcctgg
     3601 tccgcggcca cctcccccac gggcacgaag tcagggccag ggtgacgagg agggggaagc
     3661 ggatctccat caattcgttc gaagaagtcc tccccgacct ggaggaactg aatgccagga
     3721 tagatgcatt cctggcgatg acagaagcag ggaatacttt caccccgcac ggccgccaag
     3781 atataagcaa gtgagtgtac cctctctcca cgacaggtga acaactgatg agtaacggac
     3841 cgattgaact tgaagacctg cccggcgtgg gacccaccac cgcggagaag ctccgcgagg
     3901 caggattcct gagcgtggag aatatcgcga ctgcttcgcc ccaggatctt gcagagactg
     3961 ccgagatcgc cgagtcaacg gcaaaaaaga tgatcaaggc cgcccgcgag atggtggatc
     4021 tcgcaggctt ccggaccggg aaggatgtct tcgagcagag gaaagaggtg cggaagttga
     4081 agacgcgggt gccagagctt gacgccctcc tcggcggcgg gctcgagacc caggcgatta
     4141 ccgagctcta cggcgagttc gggtcaggaa agagccaggt ggcccaccag gcagccgtca
     4201 acgtccagct ttccgaggac gagggggggc tcaacgggtc tgccatcttc atcgacaccg
     4261 aaaatacctt ccgccccgag cgtatcgagc agatggtgat cggcctcggt atcgacgccg
     4321 acccccagga gttcctggag aacattcacg ttgccagggc ccacacgtcg gaccaccaga
     4381 tgctgatgat cgaaagtgcg aaggagaaag cccaggagct gaagaactcg gaccgcccgc
     4441 tgcgcctgat aatcgtggac tcgctgactg cgcatttcag ggccgagtac gcgggccgcg
     4501 gcaccctcgc cgcccggcag cagaaactga accgacacct ccacgatatt ttcaggcttg
     4561 tcgatgatca caacgcggtg gggatggtca ccaaccaggt actttcgaac cccgcggtct
     4621 tcttcgggga tcccaccaag cccattggcg gaaacatcgt gggccacacc gcgaccttcc
     4681 gcatctacct gcggaagagc aagggaggaa agcgcatcgc ccgcctcgtc gacagcccca
     4741 acctccccga gggcgaggcg gcattcatgg tggaagaggc cggactcaag cccgtataat
     4801 tctcaagcca tactcttttt cccggcacga aaccttcggg aggccatgat gtgagagata
     4861 ttgatttcat accttcacag tgcaccactc atcccatggg aacgtgcctg gcattgatga
     4921 acccttcacg cttgcgcaac ccctgacggc ggtgaggccg gagccgggga acaggtgcag
     4981 atcaaccacc gtgcgaatct gccctgccca tggattatcg agtgttggga gatgggaaat
     5041 gggagcagat tgtcttttcc ctgggtttca ttgtaaccct gctccctggc tatcttgggg
     5101 gggttgaacc tgatgcaagg gtatctcttc cacctcataa gaaccgcgaa tcattgcgac
     5161 ctccaggtcg ccccgggatc aaagggcggt accctcgcgc cgtctcccca tcgtctgcga
     5221 aaaaacaccg aggatccggg tgatcgccat ataaccatat gttcgacagg caaggccttc
     5281 gggggaaatc aggaaagaag ccctcgttca attattctca aatccgaaag gcataaggat
     5341 cccgggacga gagtccatat ccggaataca caaatgaaca ggtctttttg cctcaagggc
     5401 ctcgtcaccg atatcgatgg aaccatcacc gacccaagac ggaggatcca tacgggagcc
     5461 atcgagatcc tccgtgacct gatggagaaa ggtgtgtatg tagtgctggc aagcggcaat
     5521 acttcatgct tcatggatgc cgtctccaag atgatcggca ctcccgggac ctacatcggt
     5581 gagaatggcg gcatcataag gaacgggtac ggacaggata ttacccttct cggggacggg
     5641 gccgcaccaa ggaaggcact ttgcgacctg gttgaggcat accaaaggaa gggcatatca
     5701 atcgaacatt acagcctccc ctaccgtttc gttgacgtgg cttttgcccg cactctccct
     5761 gtcgatgaag tccgcgggat cctcgagcat caccccgtcg aagtgcttga tacagggttt
     5821 gccatccaca tccatccgcc gggtgtaaac aagggtgtgg cgttttccga gctggcacgg
     5881 gccatgggcc ttgataccag ggattttctt gccgtgggcg atgcagagaa tgacatcgaa
     5941 ctactcaggc gtgcaggcat cggtgtggct gtcgcgaatg cgcacccgca attggtcctc
     6001 tatgcagacc atgtaacaga aagagagtgt ggtgacggtt ttatcgaggg tgtaaaaaag
     6061 tatatggcct acttcctgga gaggtagcgg tctactacga tatagtcctt gatatctttc
     6121 accgcctcga acgggatata gaggcggtcc ccttcggttc ggtatcccga ggtatcaaac
     6181 gaagggtcag gatatataac caggtcaacc acctgcccgg tctcgaaatc taccaccagg
     6241 ttcttcaggg tgccaatcac cttcccctcg ttcgtgacaa tcttcttctt ggaaagacta
     6301 cgggccagga ctcgtgacat gaaaaagggt gtggccctat tcatatataa acatgtttaa
     6361 aatcgccgga atagtcgccg ttctggtaca aattcatgcg cttctgcgtg cctgaaaaaa
     6421 ggcacaatgg tccatgcaac ccgcatcgca cctagagaga cgggaagacc ttgatgatat
     6481 cggattgcgt gagtattcca accgggcgtc cctcctccat cacgatgagc cggccgatat
     6541 cacgctcctt gaaactccga attacctcga agagctgaac cgaggacggg gcaaatacca
     6601 catcgggggt catgatagta gagacaggag tgtccatcgg gacgttcttc tcgatcgcgt
     6661 ttgccacgtc gctcattgtc acgatcccga acagctcgtc cttctcgacc accggcgcac
     6721 ccctgatgtg gtgcgagttg aagagatgga gcgcattttt caggggatct gccctgcaaa
     6781 gcgagataag cgggctgctc atgtagtacc tgatcggctt tttcggcagg gatatcatct
     6841 cgaagatgga gatcagcaac gtctgagaga cctcgtcctt tccatacacc tcgccacgta
     6901 tcaggagctt gttcaccggg gtcggcccga tcgttatctg gtcccctatc ccgaatgctt
     6961 tcaccgagcc tatcagcgtg gcattcgccc ggcatatatc cgggtggcat agggtggtaa
     7021 acgcgatctc gaccaccttg acaccctgca cctcctcccc gttcctggct atcttcacat
     7081 cgtattcatt cccagagata tccaggttca gggcacggta tgcctgggca gttggattat
     7141 atcccccctt tggcccgggt attccatcca caaagccaat tgcttttaat gcctgcatct
     7201 ggttccggac cgttccaggg ttcctcttta tcacctccgc gatctcctcc ccttttattg
     7261 gatgcgaatg ctggtgatac agcgtaatca gcgtgataag gatgtctttc tggattaggg
     7321 acaaatccat aatggtttat catctgcatg ggcatatata agttaggttg aaccacagtg
     7381 gaatttgaga ccattccaac ggtgcccacc gctgacgaga ttctcgatcg tagtttccgc
     7441 cgggcggcat ccaagatgaa actcaagaag aacaaagacc gggcaaacga ggaatttgtc
     7501 cgggccgtga gccaggcaat ccatgaccgc ctggtcaagg tgatacagtc tttccccgat
     7561 tttgatgcag tctctccatt ctaccgtgat atcgtggata tcatgtgggg gcttgatcga
     7621 gtccggaagg cccttggcgg ggtgggatgg gctgccaggt gggcccgcac ccacggcccg
     7681 ggccttgcct accagaccag gaaatcggag aatccatcgc agatacgaaa gagagccgtg
     7741 gccaggctct catcggtggt gcaccaggtc gaagaagacc tgctcttttt gaacgaagtc
     7801 aggaacgtcg tccgcaaact cccccatgtc agcgatgagt tcaccgtggt ggttgcagga
     7861 tacccgaacg tcggcaaatc ctctttcatc cggctcgtct ccacggcagt tcctgaagtt
     7921 gcctcctacc cgttcaccac caagggcatc attgtcggcc accgggctgt cggaaaggaa
     7981 cgcatgcagt tcatcgatac tcccggtgtg ctcgaccgcc cgtctgatga gcgaagcggc
     8041 atagagcgcc aggccctcac cgccatggtg aacctggcct cggtaatact ggtcatcatc
     8101 gatgcaagcg agcactgcgg gtatcccctg gaggaccagt tcaggctgct ccacgagatc
     8161 gaggagatgg tcagcgtacc tgtcaaggca gtggtgaaca agtcggatct cgcgtacttt
     8221 gacggataca tcaacatgtc gacagaaacc ggtgaagggg tggacgaggt gctggaatcc
     8281 ctgcttgcat accggaagcg atcagagggc gagagccagg ccgtacccgg ctaagaatcc
     8341 aaggatcgct cccccattta ttggagggag gcctgcctgc ggttttccgc ggttgacata
     8401 gaccagcagg acagagagcc cggcaaccga gcctgccatc gcgccaagcg caggaatagt
     8461 caggaagcca agctttgctc cctggaaaaa gacgtttgcc gagacgacga gtatggctgg
     8521 catgatcatg tctcccatcc cgatgatgaa cgcacccctt tcaccttcct caagcggccc
     8581 gagtccttcc ttcctgtagc tgtatccgcg tttttttggg accacgaaga gaatggggct
     8641 tttggtctcc actacccctt cggcaagggc gatcatgtgc tttgtcttgt atacggatat
     8701 ggcgtcgtaa gccgcaagga gtatcaaaag gatgatgacc gggaggatct caagcgatat
     8761 cccgaagatt gatgctgccc ctgcggcaat caagaggccg agggtatcga tcacgtacca
     8821 ttcaggatag agataaagaa ggagtgtcgc cacgatggcc ggcaagaaac tccccacgag
     8881 ccctgccagg gaatcgtccg tcagggcata ggtaatcgac ccgaagatgt agataagggt
     8941 gaagaaaatg gagaccgcga tgatcgcacc aatgaaccgg cgaaacttat acttgatcag
     9001 cacgagcagg aacgcggtaa agacgaggag gatccctatg aagatcagtg gattcgcaac
     9061 agaggccgga tcctcgaatg ccgcaagccc ggcctgctgc attgggagag agagaagaac
     9121 tgccccgatc tccaccagga caagcatggc cggcattgca ataatgggga tgtgacgtga
     9181 gagattcatg ccctgatcac cgtacgcggg ttagttttat acattggtgc tcatcatgta
     9241 aaaccatgga tattcgtgaa tacctggggg atttactcct tcttctcatc ctggtgatct
     9301 ccaccctgat cctggtattt aatctgaact ttgatcttat caaatcgttt gcagccgcac
     9361 tcatgatgct ctctttagga gggcttatct tcctcgtgca ctggaaggtg aggcgtattg
     9421 agaagaacct ggcaaaccgg gaacggatgg tcaggatgaa catggaggag atcgcggcca
     9481 agatggctca gaagtacgac acccacgtct cgcatatcga cagcgtggtg gaagagttcg
     9541 cgaaaaggat ctatcgttga gccctgatct ggcgggaggt gtcgtttcat gggcgtagca
     9601 ttccgggata tcctcacgga atacaaggat ccagtcgggt gggagaccct cccaggcgtt
     9661 gccgctgtcg atgcccacaa cgccctctac cagtttctga gcatcatcag gcagcccgac
     9721 gggacgcccc tcatggatca ggaagggcgc atcacctccc acctctctgg aatcctcttt
     9781 cgaaccgcaa attttcttga gaaaggaatc cgtacggtct gggtctttga cggtacacct
     9841 ccagacttca agcaacagac gatcgaggtc cgccgcgcca tcagggacca ggccggtgag
     9901 aaatggcagg atgcactggc acggggcgat atcgcagagg cgtacaaaca agcccgatcc
     9961 tcttcgagga tcgatgaaga cgtgatcaag accagccggg aactcctcac actccttggc
    10021 ctcccctgcg tccaggcccc aagcgaagga gaggcacagg cagcccacct cgtcgggaag
    10081 ggagatgcaa gatacgtggt ctcgcaggac tacgacaccc tcctctttgg cgccccggtc
    10141 ctcgtgagaa acctgacagt gagcggaaag cggaaggtgc gggggaggac catctctgtc
    10201 tttccagaga agatcctgct ctcggaagtg ctcaagggac tctcgctcac ccgcgaagac
    10261 ctcgttcgga tcgcgattct gatcggcacc gatttcaact ccggtatcag gggtgtcggc
    10321 gcaaagactg ccctgaagat ggtgcagaag ggagaattcg agcgaactat gagggagaag
    10381 ctcccgaaca cggactggga gccgataatg gagttcttca tgaaccctcc cgttacagag
    10441 aattattcgc tccagtggaa ttccccggac ggggaaggga tcgtcaggat gctctgtgac
    10501 aggtatgact tttcgcccga gcgtatccag aaggcccttg acggtatggg tgcgaagtca
    10561 gggcagaaaa cgctcgattc ctggttttcg tagcatctct tccactatcg gattcacttc
    10621 cccctttgca gggcatggtg gagcaaaatg tcatattttt ctttcaacga agattccatc
    10681 gggcgagaga aaagttagtc agggcatctt tctccacacg taaaagaagc gctgtgccgc
    10741 ggtaacgtgc cccataaccc caaataaaag caggagccat ccaaggatcg agaggccgaa
    10801 gaacgtatcc gggacaaaca ccgtgagcac ccctgcgcaa aggatcagca cgaggcgatc
    10861 cgccctcccg aggactccgc cataatatct ccccacaccc acggcttgtg cctgtgtccc
    10921 gaggtatgat gacatcagca cccccgtgag tgccagcact cccacctgcc agctggcaaa
    10981 ccccccggca aatatcccgg tgatgatgaa gatatccgca tacctgtcca ccacgtgatc
    11041 aaggaagtcg ccttttgggc cctggatgtt ctctgcacgg gcaagggcac cgtcaagccc
    11101 gtccaggaga gcgttaaaag ccacgaacgc agtccctgcc agaacatttc caaggtagaa
    11161 gaagaccccc gctgcagccg cagtgatcag agcaccgatc gtaaggttgt tcggggagag
    11221 cccgatcttc ttcgagaccg agatgaaggg ggccatgatc tccttcatgt gcggacgaag
    11281 gatctcgagc gtcatttcgg ttctccgaga taggccgacc agtcaatctt cccgaacgaa
    11341 ggaggcacct tgccgcgtgc aaattcttct atcaaccctg cgcactcccc gatatcgcgc
    11401 tcggtcgtat caatctccag cacctggtcg ccgctgaact cttccagtgt ctctatcagg
    11461 gaaacatcga tcgcctctgc ctccacgttc tcggaaacct tcgcctccgg gtatccgcgt
    11521 gcacggagac gggcggcgag cacatcgggc ctgcagcgga gcacgacgat gagatcgcag
    11581 ggtaggagat gcgccaggtg cccttccacg aatccctcga ccttctggaa ctcactggcc
    11641 caggcctcct cgtcgatgac gagtgtatgc ctggcctcgt ctcttgcgag tatgtaggga
    11701 gtggtggtgg tggagagcga ggtaacccgg tatcccctcg ctttcagttc ctgagccaca
    11761 gttgactttc ccgttcccgg cgtcccggtg atcccgatca tcatagcgcg ttgatcacgt
    11821 ccaggaacct ggcattctcc cattcttcgc cgatgctcac ccggatatag tgtggtccaa
    11881 gcccggggaa gctctcgcag gaccgcacaa ggacccccct ttcagcgagg cgatcggcca
    11941 tctcccttcc gctcgaaggg gacacatcca ccatcacgaa gttcgcaccc gaatgaaaga
    12001 ccgggtattt gcattcccgg acgaccagtt cacggagcct tttcaccccc tcgaccgtct
    12061 ctttgaggtg caaccgatcg gcgagcgcac ctgttgctgc agccgcagag accgagttta
    12121 cagtaaatgg tgtggctgcg cgcaggtagc agggaaccag ccacagggga acgaacgcat
    12181 agccgacacg taggcccgcc agagagaaga cttttgagaa cgtccggccc aggatgaggt
    12241 tctcataccg gttcatcagt gggcggtaat cctcgtcgca gaactctacg tacgcattgt
    12301 cgaggaaaag gaccccatgt atcgcggaga ggatctcctc cacgtcatcc cggctggtca
    12361 cattccctgt tgggttgttc ggggtgcaga ggaacgagat cttcgcatcc cttgcactgc
    12421 ggacgaattg atgcacatca accgagaaat cgggtctcct cgggctattc accacctcgg
    12481 caccctgtgc catcgccgcg aggccgtaaa aggagaacgt gggtgtcgag accgcgactc
    12541 tctccccagg gcataccagg gtccgtatca cggtctcgat caccccgtcc atccccacgc
    12601 cggtcacgaa gtggtactgg ccatggaact tcgcaagagc cctgacaagg tcggccattc
    12661 gttcgtcagg atagcggttt acctcgcgca gggcctggca gcccctctca agcgcaaggg
    12721 gagacggggg acgagggttc tcgttgctcg cgaggcgggc aacccggcag gcttcgtcgt
    12781 cctctcctgt attccccgca cgcctggcaa agacatatcc cccggagcgg taacaatccc
    12841 gcacaagcga ggtgtacctg gaatcattct ctttcgccat tccgcccccc gtgtaagtgc
    12901 ttccagtata agctctccac tcttaataag ggcgttgcag caggcgggcc gggtgccggg
    12961 ctcgcaatcg gaccctcatc atgccgccac aaccttatga tctggcttga gaaaagggaa
    13021 agacccgaac tcttttatgt gtaaatcaga tacaactgta aaaatggggt tgtacacatg
    13081 gaacagaaca gactatacac actgccggca ctgccatacg actacaaggc gctctccccc
    13141 catatctctg aagagcagct gcgaatccac cacacaaaac accaccaggg ctacgtgaac
    13201 ggcgcaaacg caatctacga gaaactcgac aaggcccgga gagagggaag cgaccttgac
    13261 gtgaaggccg tccacaaaga actctctttc cacattggcg gcttcctcct ccatgcactc
    13321 tactgggaga acatggctcc tgcggggaag ggtggcggtg ccccgaaagg gcccctttca
    13381 gctcatatcg agaaggactt tggctcgttc gaacggttta aaaaagagtt caccactgca
    13441 gccaacagcg tcgagggctc cgggtgggca gcgcttacct tctgccagaa gacagggagg
    13501 ctactcctga tgcagatcga gaagcacaac gtcaatgtat acccagcgtt tcccatcctg
    13561 atggccatag atgtctggga gcacgcctac tatgtcgact acaagaacga ccgcgcaaaa
    13621 tacatcgata acttctgggg catcgtgaac tgggatacgg tgaatgcgag gctggaaaaa
    13681 gtacttaaaa aataaataca ctcgttttta gcccagtgcg tctttgagaa cggaacatgc
    13741 ccggtcaatt tccgcattgg tgataacaag gggcggcacc aggcgaaggt tgccgtcggc
    13801 ggcacagttg acaagcaccc cgttctccgc acacctcttc tggacttccg cacaacggtc
    13861 cccgaccgtc atcccaacca tcaggcccct tgccctgggt gcatacttcg agagcgcatt
    13921 gcagaaccgc tcaccttttt cagagaccgt gggcaatatc tcatcgatta ccccaaacgt
    13981 ggctaacgct gccgcgcatg ccagtggacc ccctgcaaat gtgctgccgt gttctcccct
    14041 gcggaactct aggccttccc gcgcgagcag ggcgcccatg gggaaaccgc ttgcaatacc
    14101 cttcgcgagc atgacaatgt caggcttcac acctgcatgc tggatggcta accacttccc
    14161 ggtccggccc atgcctgtct gcacctcgtc catgatcatg agggcgccgc tctcgtcgca
    14221 gatctcccgt atgccgtgga ggaaactgtc aggagggatg atgacccctg cctcgccctg
    14281 gataggttca accaccaccc ccgcggtgtc actgtccacg accttcctca atccgtcgat
    14341 atccccatac tcgacgaacg tctttgatat accaaggggt tcgaacggtt cgcggatcgc
    14401 gggcttgtgc gtcacggcaa gggatccaat ggttcttcca tgaaatccgt gcgtgaaggc
    14461 tacgaatttt ttcctccccg tcctgacacg ggccagcttg agcgccccgt ccgtcgcttc
    14521 ggcccctgag ttcgagaaga acgcttttgc cattccggtc ttctccacca gtttctgtgc
    14581 cagttccgcc tgtcctggca cataatagag gttcgagcag tgtatcaggc gctttgcctg
    14641 ttcgcagatg gcgcgggtca cggcgggatg gcagtggccg gtgctgcaga ctgcaattcc
    14701 tgccacgcag tcgatgtatt cttttccgtc ggcatcccag accctcgagc cctctcccct
    14761 gacaatcatc aggtcacggg caaacgcggg catgtagtac cgggcttcga ttgcccggta
    14821 gtcttccttg ttctgcatga tatcgcacta ctgggtaact gttctcttat tcatactcga
    14881 ttgttgcagg gggtttggga gtgatgtcgt acaccaccct ggccacgctc gggatctccg
    14941 aagtgatgcg ggaggcgatc cggtccagta catcccaagg gatctggacg ggatctgccg
    15001 tcattccatc gcgcgagttg acggccctga cagccaccac ccacccgtgc accctgttgt
    15061 cacccttcac ccctgtcccg agtccgagga gggccgcgaa gcactgccat ggattgaact
    15121 gctcgacgat ctctcgctcc acgatccagt tggcctgcct gacgacctcg atgttctctc
    15181 tggtgacttc gcctataacc cggactgcga gtcccggccc tggaaatggc atgcgatgct
    15241 gtatctcgtg ggggagtccg agtgcacccg cgagcacccg cacctcgtcc ttgtacaggt
    15301 cccgtagggg ctcgatcacc gtctcgaact ccatgtggag gggcatgcct cccacgttgt
    15361 ggtggctctt gattcccccc tcgctctcta tcctgtccgg gtagatggtc ccctgaagaa
    15421 gcgcccgtgc accagacctc ctcgcctccc gttcgaagac acggataaat ctttcaccga
    15481 taacctttcg tttctcctcg ggatcgcgaa cacccctgag tgcccccagg aactcctcct
    15541 ccgcaggcac tacgtgcagg tgaagttttc caaagacctg ggagatccgc tcggtctccc
    15601 ccttacgcat cagacctgtg tccacgtaaa tgggctcgag cctgtccccg attgcccgcg
    15661 atgccagtgc cgcacagaca gagctgtcga caccgccaga gagggccatc accacccttc
    15721 gttcccctgc ctcgcgccgg atctcctcga cggcattcga aatgaatttc tcagcgttga
    15781 tcatgttctg tcgcacctca cctcgctctt ctcccgttcc ggctggagca tgccttcacg
    15841 aaaccaacga acggtggaga cggtctcgtc ggcctcgact tgaactcggg gtggaactgc
    15901 gtggccatga agaatggatg atccggtatc tcacagatct ccatccggtt ctggaacaac
    15961 cccgagaaga ccagtccagc cttctccagg tcaggaataa atcttggatt gacctcgtac
    16021 cggtgcctgt gccgttccgt aatcgcgctc tgcccgtata cctgcatggc aagcgttcct
    16081 tcttttaagg tgatcggaga atccccgagc cgcatcgtcc cgcccagttc ttttacttca
    16141 tgctgttcag gcaggatggc gatcacatgt gtaccttcgc caatctcggc actcgtggcg
    16201 tcctcccacc caagcacatg gcgggaatat tcaaccaccg ccatctggaa cccaaggcac
    16261 aggccaagga aaggtgttct gttggtcctg gcccattgga tggcatccac tttgccctct
    16321 attccccgtt ttccaaatcc tcccgggaca aggatcccgt cgaattccgc aagttgcttc
    16381 tgctcgtacc gttccgcgtc gagccatgtg atcctcacct cagtggaaag cgccctcccc
    16441 gcgtgtttca gcgcctcctt gatgctcaga tacacgtctt caatcccata cttgctcaca
    16501 atccctacac tcacccgatc ggtgtattcc ctgctgacca ggtgatacca ggaagtgtcg
    16561 gccctccgct tctccaggcc aagatgttcg gagaggacgt ccccgagacc ctccttctcc
    16621 agctcaagcg ggacctgata gatatccggg acagtcgccg cactaatgac acccttgaga
    16681 ggaagatcgc agaacgccga gatcttgcgc ttggtgtggg acccgatggg gcgttcgctc
    16741 cggcccacaa tgatgtctgc atggagaccg agttcgcgaa gggccttgac cgagtgctgg
    16801 gtgggctttg tcttcaggtc ccccatggta tcctcgggaa taagcgtgac gtgaatgatc
    16861 actgtgtcct gcagggtgag atcgccccgc atctgcctga cagcctcaag aaacggcata
    16921 ctctctatat ccccgaccgt gcctccaatc tcgacaaggc aaatatccgc tttcttctgc
    16981 cccgggatct ccatatcagc agcggcacgg atgcatgcct tgatctcatc ggtgatgtgc
    17041 gggatgatct ggaccgtctc gccaagaaag tcgccacgcc gctctttctc gatcacggtg
    17101 cgatacacct tccccgtggt gatgttatgc gcagatgaga ggtcgatatc caggaagcgt
    17161 tcatagttgc cgaggtcaag gtcaacctcg cttccgtccg aaaggacaaa cacctctccg
    17221 tgctgcccgg ggttcatcgt cccggcgtcg atattcaggt agggatctat cttgacggcg
    17281 gtgatcccat accccctgtt cttcaggata cgccccaccg atgccgcggt gatgcctttc
    17341 ccgagcccgc tcataacccc gcccgtgatg aagatgtatt tcacaggaat ttcccctctg
    17401 accctctcat gcttgccatt cccggcatca ggaaggccgc aatgcggcgg cgcctcccgc
    17461 ggtccccggt gctcatgatc tcttcctctc ttttatgcct ccaagagaga tagttgtatc
    17521 ccgtcccatg gcctgcaggg ggatggaacg ctctccgatc ccctccgggt tgccggaata
    17581 cccgtatctc acgtgcgatg gccggctctc accgcgaacg ctgcggtgct ccgggagagg
    17641 agcgggccgg ctcttgcacc atccctgacc tccccctcgg caaataccac tctcctccct
    17701 ttttttacca cttttccgag cgcatacagg cagccctccc gggaaccgcc gatgaacgtg
    17761 gtagactcgg tgatggtcgc gatacggtca tcggggccaa tgagggaata gattgccagc
    17821 accattgcct cgtccgcgag cgccgtgaat actcccccct ggagccatcc ctctccgttc
    17881 agcatgtccg gtcggactgt catggagata atcgcctcgc cctcccccgc atgctccact
    17941 tctatcccca tcagaccgaa gaatgggttt gccttgcttc ccatctcccg gatacgtgca
    18001 aggtagttca tacctgga
//
LOCUS       DAOQYR010000042        17775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_3568_length_17775_cov_9.430248, whole genome shotgun sequence.
ACCESSION   DAOQYR010000042 DAOQYR010000000
VERSION     DAOQYR010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 17775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17775
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3568_length_17775_cov_9.430248"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /gene="larB"
                     /locus_tag="PKM87_06430"
     CDS             complement(<1..656)
                     /gene="larB"
                     /locus_tag="PKM87_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HNQ29740.1"
                     /translation="MSPNHRVREVLSLFREGQISLDDAVDDICGLRIEAVAALANLDT
                     GREARCGVPEVVLAEGKSPSQVGEIARNVTAAAGRIIFSRLSPDHVEALIDVCQDCGF
                     SLTLHEQAAMAVVSTGPPTEPGKGVVAIITAGTSDIRVAEEARVIASEMGCRVKTAYD
                     VGAAGIHRLFPALRECVGAHVYIVAAGREGTLPAVVAGLVDRPVIGVPVGTGYGYMGR
                     G"
     gene            706..1044
                     /locus_tag="PKM87_06435"
     CDS             706..1044
                     /locus_tag="PKM87_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ29741.1"
                     /translation="MSYLIYVAAFGTLAVFFLWLRDVRIFLRTGLPGYRNAAYRGVLH
                     GALAILGCAFTFSSTERFPTELIGLGIILAALYFQGKVTRERVFTKESTWDRVAGNAP
                     ARKRTAEPEE"
     gene            1055..2287
                     /gene="hisS"
                     /locus_tag="PKM87_06440"
     CDS             1055..2287
                     /gene="hisS"
                     /locus_tag="PKM87_06440"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNQ29742.1"
                     /translation="MLQKPRGTRDFLPDEMESRREVERVMREVARTWGYREICTPEFE
                     ELEIFTIRSGEGIIQEMYVFEDKGGRKMALRPELTASVLRMFVNEGRTLPKPLRWFYF
                     ADCFRYERPQKGRYRQFWQFGVELIGADSALAEAEVIMLAHEILSASGISFSLHVGHL
                     SFMRSLLAELEPDDQKRARMFLDKKDWRGLEDYLSSRGESGLSERLISLAECRDLREV
                     FSITGENPERKRIEDLFCVLDQSRIPYILNPGIARGLDYYTGMVFEAFAEGLGAENQV
                     LGGGAYRLAHLFGGEDTPSCGFAAGFDRVMVARGARPLSKGVSVGVAFTPEGREWALA
                     AAKEFRNIGIRTHLDLSGRSIGQQLSHISRTADYALVIGSRERDERSVTLKDLRAGTQ
                     KMLSLPDAVSEVSGADSR"
     gene            2274..4079
                     /locus_tag="PKM87_06445"
     CDS             2274..4079
                     /locus_tag="PKM87_06445"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HNQ29743.1"
                     /translation="MTLADDLARQQRSISVAEFFEKNKHLLGFDSPTRGIITTIKEAV
                     DNALDACEEAMVLPDIFVGIKRTESDVFRITVEDNGPGIVPAQVPFVFGKLLYGSRFH
                     QIRQSRGQQGIGISAAVLYAQLTTGIPTIVTSRTGPENPAFRFFIQINIKTNEPEVLE
                     QSEVEWDRTHGTRVQIEFKSTMAAKKRLLEYLKYTSVVNPHARIRVELDDETYLFERV
                     SDEVIACPQAIKPHPHGIEIGQLMRMLGAAEGSLGEFLIESFTRVGKKTAEEMCRSAE
                     ISPGTLASSLSPIEQKRLLSAMQGVKVPAPPTQTCLSPIGEDLITRGLEKEFQMDFVR
                     ARTRPSSVYSGHPFLVEAAIGYGGKLPPDGNAQLLRFANRVPLLYQQGACAITGCIAG
                     VSWKSYNVSQAGLPTGPLLILVHVASTNVPFTSESKDAIAAIPEIEREVTLVLQELGR
                     ELKTYLSRRDRNRLQEDRARAVCAIIPEIASKVAEIVDLPVPDISPIEGRIMKKVVVR
                     KSALEGKVTLDVKNYTSQPGAITLYDTSPERGGDAYPKPAIVTELDGEYTKVWQVELP
                     PGGGWTAVYSGSGGGSLEVRGIPPEHVVVVDLDVQ"
     gene            4069..5160
                     /locus_tag="PKM87_06450"
     CDS             4069..5160
                     /locus_tag="PKM87_06450"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HNQ29744.1"
                     /translation="MSSDEMDQKTQQRLVSIAMRWYEQMKEGAIPSISLPTRTKQNIE
                     FDDDAEVWKYGDRESIRSAHTAKSALHLLKMSYVIAFLKKQLHERRSSTLRELYYISE
                     GWKRAKFSAQDESNLLIEDLEILTDLQREAFHLRPEEDGATIFGPLRLKEMTRRGERV
                     IHCQEDVGEAGYQIPSNVENIDLLEHDARFVIAIETGGMYARLIENGFDQEHQAILVH
                     LKGQPARSTRRMLHRINEAFGLPVLVFTDGDPWSYRIYASVAYGAIKSAHMSELLATP
                     RAQFIGVQPSDIRDYNLPADTLTDKDIAALQSEISDPRFSSEYWKSQIQLQLKLNLKS
                     EQQAFAARGLDFVTKEYLPRRLTEMGILS"
     gene            complement(5448..6434)
                     /locus_tag="PKM87_06455"
     CDS             complement(5448..6434)
                     /locus_tag="PKM87_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNQ29745.1"
                     /translation="MDLSQKEIGQFAREQVEITSKLERHRSQRSGWRKVLKHPVQVLL
                     EKPLNILILTLPLAALLFLIGTAFTIRDYGMSVLFTTTLIDDIVIISILVAIVPVAIM
                     DFRESRRMKNLEEALPNFFRDLAGMNDSGMTLPNAIHLVAQGEYSTLTPFIRRLDNEM
                     SWNTTFIEAIQRFRARLGTPLSDRSIDLIAKASKAGGDVSEVLRAAARDTYEVLALRS
                     ERKNNMLIYVIIVLISFMVFVFVILILVSTFLSTMATAGSAASAAGAGAASFIGNVDL
                     FFYTRLFSHAAMIQGIFSGLVAGQMGEARVVAGLKYSAIMLVIAWLVFRFMV"
     gene            complement(6640..7563)
                     /locus_tag="PKM87_06460"
     CDS             complement(6640..7563)
                     /locus_tag="PKM87_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNQ29746.1"
                     /translation="MNGFERLAFRIMGGYAEKKRDAYSTLRNDLVTARMKVPFEVYLS
                     TAYLSSVIAGIVASIVLGLITYLLNLPAMIKYQGAVPEFMLTINEYSLIIGTVLAIII
                     SFAVIGGITFLIFLIYPSVVAGGRCRNIDATLPYAINYITAMSTAGIPPAEIFRQLGS
                     STMYGESAVEARFIGMEIDLFGRDLIDALRLISATTPSMRMKEFLQGAMGCISAGSSL
                     TEYFRTKAEQYSLENRQTQKLFLDTLGLISESYVTAMVAGPLFLIILQSIMAILSQSS
                     QPIFLYIIIYMMIPFGSIMFVILISSMTPEG"
     gene            complement(7560..9815)
                     /locus_tag="PKM87_06465"
     CDS             complement(7560..9815)
                     /locus_tag="PKM87_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HNQ29747.1"
                     /translation="MTAEDKKGLSFRDLFGRKKGEGPADKPKKEGTQEKNEVSVPPVE
                     REDLDGFMKRLGLSLDTKKTPSPGPQEKVPSGIEPGEEMPPVEVVEKPLAGLDSQKKA
                     LDISAPAEIPPIEKKETVTDRSPPRFEPVKKTVWSEKKPVLEETVISVDQINDLSGLI
                     LPKGATFQIEEIKIQDRSNIFSFKGESGAVAGKDLQKTDLRDSSLKKVSDAAIEVHEE
                     AGKGGLLARLNPINVIHRVPVEYNPKTHGPLVDLTFRPRPGIEQTELYPVNEPYAYVR
                     ITYDNATHEYLYEVLEPKLTPAEVDLFRDIKERIFERLDINTQNVLEEIAKKTLRDVT
                     DDIIHDYGLSLSPVQREKILYTMYKEFLGNGMIDPLMHDRYIEDISCDGVNANLFVYH
                     NRYESMRTNLLYKSSDELDSFVTKLAQRAGKYISIAEPMLDATMSDGSRIQMTLGTEV
                     TAHGSTYTIRKFKDEPITPTDLIEWRTFSPLSIAFFWMAVESGKSCIFAGGTASGKTT
                     SLNAISLFMPPLAKIVTLEDTRELKLPHPNWIPSITRTSFDTSGKGEIDMYELLRAAL
                     RQRPEYILVGEVRGKEALTLFQAMSTGHVTYSTIHADSVASIVHRLENPPLNVPRNML
                     SALDLVSVQVQARIGGQRIRRNKQIIEILDIDPRTNELITNEVFRWHPATDEITYSGK
                     SYVLEEIMESRGWDEDRMREELKMRQEVLEWMRIKKIRNFRDVGNILVQYYRDPAGTM
                     EKIRAELYGGA"
     gene            complement(9880..10578)
                     /locus_tag="PKM87_06470"
     CDS             complement(9880..10578)
                     /locus_tag="PKM87_06470"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNQ29748.1"
                     /translation="MSLPEGTYAGVSSDPVQEDRPGGASPFTGKVEILTDEGAGLLIV
                     DNGRVDAAYYHDNSGDYRGKDAVVHLQHRSEEAGLSIRYIQYRYSPGQASDAIQICRR
                     EGLSLFEEDTHAKKPVRAGLDDALIARLSGQPGVRAVSIFFEGFPVQSVGDGDFEQVA
                     AMAEDFLRAGRKMAENIRMGVPDQITLESDQGKCIIAPYGDLFLCLLTDSDANIGLIR
                     LAIRTIQKELKGRV"
     gene            complement(10582..10929)
                     /locus_tag="PKM87_06475"
     CDS             complement(10582..10929)
                     /locus_tag="PKM87_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNQ29749.1"
                     /translation="MLKQILGEFLKIDGVTAAVVVGRDGFVIESAASGKMDIDALGAM
                     ASTGMGTSEAMGKELGKGELNQMLVELDHGPILMSPLSADELIAVVAGNEANIGRIRY
                     ELKKNKERLVAAL"
     gene            11096..11764
                     /locus_tag="PKM87_06480"
     CDS             11096..11764
                     /locus_tag="PKM87_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29750.1"
                     /translation="MQLPRGTFHSMKKGLELGTILLKLKDENFSGSCSLVCESQAIDM
                     VFQDGRVMLASCDTSCGDAAMDIISQKQDCIVDASLSDLTPAQLRLTLEFNNGCRVRR
                     QRPSAGKTPAPAPDGWTPVPNQETQKVTSQSPPVKNTGKMPAISSSPVPAPVVIPRHQ
                     GERERGPAGSPALDGDEYTIVDRDLDALDAMDLDTMSEKIRMNCKTMIEKLHLEHLID
                     DSGK"
     gene            11777..12349
                     /locus_tag="PKM87_06485"
     CDS             11777..12349
                     /locus_tag="PKM87_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29751.1"
                     /translation="MAAVSFDTFILLVLLVLVGIVLFLSLVTAIHVIRLSREIAFRQK
                     REEKATGRNAPPAGSDVKTQADTVAAATYTPVAGRKRDFDVTQDAPDIPTGMKNLCAH
                     YDLTSLSISSPDGLLIASSGYPGAINDAAQYSYAFQTGVPIKESGVQVFPVDFGTSPL
                     VGILRSTSALPDTSVDSLQDDIAALLKRWI"
     gene            12424..13209
                     /gene="minD"
                     /locus_tag="PKM87_06490"
     CDS             12424..13209
                     /gene="minD"
                     /locus_tag="PKM87_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATPase MinD"
                     /protein_id="HNQ29752.1"
                     /translation="MVRVFTIASGKGGTGKTTVTVNLGTSLAQFGKNTIIMDADIGMA
                     NMGLALGLEDVPITLHEVLAGKANVHEAIYDGPADVKVVPSGISLQGFQQANPERLKD
                     VLKELVEVCDFLLIDAPAGISRDGVIPLAVADDVILVVNPELSSIVDALKTKILTEVV
                     GGHVLGVIINRAGIEKTEVVKQKMEKVLGVKVIGTIPEDPNVRKAASFKTPVVIKYPL
                     SSSSVAIKNIAADLAGIPVEHRGVESQERFIERFAKVLFRGKK"
     gene            13206..13403
                     /locus_tag="PKM87_06495"
     CDS             13206..13403
                     /locus_tag="PKM87_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29753.1"
                     /translation="MSFEMILLIISSVVILFLLILIIFMYIRIRQLIRDFELLESQIM
                     VTDSEFDALMQNVDEIGHLKI"
     gene            complement(13606..15159)
                     /locus_tag="PKM87_06500"
     CDS             complement(13606..15159)
                     /locus_tag="PKM87_06500"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HNQ29754.1"
                     /translation="MDKQDFEAILNGSKDITSYMQMNPLEGVTTCFGVRTASNPNYMV
                     KALYEMYEANLNRKLANKAMRKLFRSVGQGSVGLDKLLKKLGLNLKPEEFLMLVFKLQ
                     HQQGWGSPFELVSASDKRIVLRCKQTFESEVLKDWKMPVCGLHQGWIEGILSAITGKT
                     WYCLEKSCHANGDPYCEFVADRVEPSWKFKAEAVVRGESAITEFIEHKPLEGKISLMD
                     EPVVMMPRFIFTSMTNSLKKTMGEAPASGVNYRAYMDMGKENVEHYKKMSIYFDLSRT
                     KLDGDLKLLHDRFAAYGSPSITGGKIAYGDHVVNDNFEVVDSIEKERGSKATVFQKTG
                     DKAIRISTNVIGADGKRAVGTEISSVVYNAVINKGQTYYGTADVVGKKYVVAYEPIRN
                     PAGEIIGILFVGTPEDMIKEEGQKKINPKTLSDMAFAFYSQMGWFNIVKEEWDEKTKT
                     KTITLSHTVESESFGNTGKPVCFCTAGLLAGIIEGSFGIKVQCREITCKSKGDEHCVF
                     QIKNRQGES"
     gene            complement(15263..15886)
                     /locus_tag="PKM87_06505"
     CDS             complement(15263..15886)
                     /locus_tag="PKM87_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29755.1"
                     /translation="MTSIEILDSLIAESAGDGPASVQALLQMARSKGVYGIARAVERD
                     QRYYILFFAGEPDGAVFNDRKGMLFGNKALYILKGTEQFTFYPVDRAIIERIILGCRI
                     FDRNILDRMLPSDIPQVTPKREGGAGVFAMRVVKEGKPVSGQRVSIRKGGQIVGNDFT
                     SAEGRVSFRLLYDRYECVVHLRDLSTRVYEFEFHPGLLNQVVDLDIS"
     gene            16050..16841
                     /locus_tag="PKM87_06510"
     CDS             16050..16841
                     /locus_tag="PKM87_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HNQ29756.1"
                     /translation="MIIPLAIGSAAVLCTFLYASCRDLKERRVPFRTWYPMLAVTLPM
                     AAWFYASFLIRGEWTIPAYFLALTAIFALVFYLFAWLNLFGGADAWALIFLSVSIPAF
                     PIEPLSGYPPAGFFPFAVLVNALLLNLFTPLLLGLQNLLHGRRAPFPYMLLGYPVPAV
                     ELPGAYGFIMEDIEENEDGSITRRFVRPLEAVRRMFSGEKRIYTKDLRLHPDDYSKEM
                     ALFKLAGQVWISYGIPFIVPLTAGFLSALFFGDILFFLIKSVSGV"
     gene            16843..17211
                     /gene="hisI"
                     /locus_tag="PKM87_06515"
     CDS             16843..17211
                     /gene="hisI"
                     /locus_tag="PKM87_06515"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNQ29757.1"
                     /translation="MLKLSYADGLIPVVVQDAGTRAVLMVAYANAEAVELTKNTGFAH
                     YFSRSRKKIWKKGEESGHLQKVLRILVDCDEDCLLYEVEQAGAACHMGYRSCFFRTIN
                     GDIVEEKVFDPEMVYAKKGH"
BASE COUNT         4041 a         4890 c         4749 g         4095 t
ORIGIN      
        1 cccctgccca tgtacccata tcctgtgccg accggaactc caatgacggg gcggtccacc
       61 agacctgcga ccaccgcggg aagagttccc tccctcccgg ccgcaacaat gtatacatga
      121 gcaccaacac attcgcggag agcggggaag aggcggtgaa tcccggcagc tcccacgtca
      181 tatgccgtct tcacacgaca tcccatctcg gacgcgatga ccctggcctc ttccgccacc
      241 ctgatatccg atgtaccggc agtgataatc gcgaccaccc ccttcccggg ttctgtgggt
      301 ggaccagtgg agaccactgc catggccgcc tgttcgtgaa gagtcaggga aaagccgcag
      361 tcctggcaga catcaataag agcctcgacg tggtccggag acaaccggga gaagatgatc
      421 ctcccggcag cggcagtcac attccgtgcg atctctccca cctgggacgg ggactttcct
      481 tccgccagca ccacctcggg taccccacat cgggcttccc ttcctgtatc aaggttcgcg
      541 agggcggcaa cagcctctat gcgaaggccg cagatatcat cgaccgcatc gtcgagggag
      601 atctggccct ccctgaaaag ggaaaggacc tccctgactc ggtgattcgg ggacatgctg
      661 tgataattaa gatatgggcc tgggtataat aagtgatgca actgcatgag ttacctgatc
      721 tacgttgccg cgttcggaac gctggcagta ttcttcctgt ggctccgtga cgtgaggatc
      781 ttcctgcgca cagggctccc cggatatcgg aatgccgcgt accgcggggt gctccatggg
      841 gcattggcca tcctcgggtg tgcgtttacg ttctcctcaa cggagcgttt ccctacagag
      901 ctgattggcc ttgggatcat ccttgcagcc ctctacttcc agggaaaggt cacgagggag
      961 cgtgtattca caaaggaaag cacctgggac cgcgtggcag ggaatgcccc ggccaggaag
     1021 agaacagcgg aaccggagga gtgaggtctt ttccatgctt cagaaaccac gaggaacgcg
     1081 ggattttctc cctgacgaga tggaatcgcg gcgtgaagtc gaacgcgtga tgcgggaagt
     1141 tgcccgtacc tgggggtatc gcgagatctg cacgccggag ttcgaggaac ttgagatctt
     1201 taccattcgc tcgggagagg ggattatcca ggagatgtac gtcttcgagg ataagggggg
     1261 caggaagatg gcgcttcggc ccgaactgac ggcgtctgta ctcagaatgt tcgtaaatga
     1321 agggaggacg cttcccaaac ctctcaggtg gttttacttc gccgattgtt tccggtacga
     1381 gcgtccccag aaaggccgct accgccagtt ctggcagttc ggggtagagc tgatcggtgc
     1441 cgattcagcg cttgcggagg cggaggtcat catgctcgcc cacgagatcc tctctgcgtc
     1501 cggtatttca ttctccctcc atgtcggcca cctctcgttc atgaggtcac ttctcgctga
     1561 gcttgagccc gatgaccaga agagagcgcg gatgtttctt gacaagaagg actggcgcgg
     1621 cctcgaggat tacctctctt cccggggcga atccgggctc tctgagagac tcatttcact
     1681 tgcagaatgc cgagacctca gggaggtatt ctcaataacc ggcgagaacc ccgaaagaaa
     1741 gaggatagaa gacctctttt gcgtactcga ccagtccagg atcccctata tcctgaaccc
     1801 aggcattgcc cgcggcctcg actactacac cgggatggta ttcgaggcat tcgccgaggg
     1861 actcggcgca gagaaccagg tcctcggggg gggcgcttac cggcttgccc atctctttgg
     1921 tggagaggac accccctcct gcgggtttgc ggcagggttc gaccgtgtta tggtagcccg
     1981 tggggcgcgt cccctctcaa agggcgtgtc tgtaggggtg gcgttcacgc ccgaagggag
     2041 ggaatgggcc cttgctgcgg caaaggagtt ccgcaacatc ggcatccgca cgcatctcga
     2101 cctctcgggg agatctatcg gacaacagct ctcgcacatc tcccggactg ccgactatgc
     2161 actggtcatc ggttcgcggg agcgtgacga gcggtccgtc acgttgaaag atctcagggc
     2221 cgggacgcag aagatgcttt cactccccga cgcagtctct gaggtgagtg gcgctgactc
     2281 tcgctgatga ccttgcacgc cagcagagga gcatcagcgt agcagagttc tttgaaaaga
     2341 acaaacatct cctggggttt gactccccga cacggggcat catcaccacc atcaaggagg
     2401 ccgttgacaa tgccctggac gcctgcgagg aggccatggt cctccccgac atctttgtgg
     2461 gcatcaagcg gactgaaagc gacgtattca ggattaccgt cgaggacaac ggtcccggga
     2521 tcgtccccgc acaggtacct ttcgtgtttg gaaaactcct ttacggctcg cggttccacc
     2581 agatacgcca gagcagggga cagcagggga ttggcatcag tgccgcagtg ctctatgcac
     2641 agcttactac aggcattccc actattgtga cctcccgcac cggcccggag aatcctgcct
     2701 tccggttctt catccagatc aatatcaaga ccaacgaacc cgaggtcctg gaacagagcg
     2761 aagttgaatg ggacaggacc cacggaacac gggtccagat cgagttcaag agcaccatgg
     2821 ccgccaagaa gcggctgctc gagtacctca agtatacatc cgtcgtcaac ccccatgccc
     2881 gcatacgcgt ggaactcgat gatgagacct acctcttcga gcgcgtcagc gacgaggtga
     2941 tcgcatgtcc gcaggcgatc aagcctcacc cgcacggaat cgagataggc cagctgatgc
     3001 ggatgctcgg ggcagccgaa ggaagcctcg gcgaattcct aatcgagagt ttcacacggg
     3061 tggggaagaa gaccgccgag gagatgtgcc ggagcgcaga aatctctcct ggcaccctgg
     3121 cctcatcgct ctcccctatc gaacaaaaga ggctgctctc tgccatgcag ggggtaaaag
     3181 tccctgcccc tcccacccag acgtgcctct cccccatcgg cgaagacctg atcacgcggg
     3241 gacttgagaa ggagttccag atggacttcg tgcgggcgag gacacggccg agttcggtct
     3301 actccggcca tcctttcctc gtggaagccg cgatcggtta tggcggaaaa cttcctcccg
     3361 acggaaacgc acagctactg cggttcgcaa acagggtccc cctcctctac cagcaggggg
     3421 catgtgccat caccgggtgt attgccgggg tctcctggaa gagctacaac gtctcccagg
     3481 ctgggcttcc caccggcccc ctcctcatcc tcgtgcatgt cgcctcgacg aatgtgccct
     3541 tcacgagcga gagcaaagac gccattgccg caatcccgga gatagagaga gaggtaaccc
     3601 tcgtcctgca ggagctcggg cgcgagctca agacctacct ctccaggagg gacaggaacc
     3661 ggctgcagga agaccgggca agggcggtat gtgccatcat cccggaaatc gcctccaagg
     3721 tcgccgagat cgtggatctg ccggttcccg acatctcccc tatagagggg aggatcatga
     3781 aaaaagtggt ggtgcggaag agtgccctgg agggaaaggt cacgcttgac gtgaagaatt
     3841 acacctcaca acccggcgca atcaccctct atgacacctc tcccgaacgt ggcggggatg
     3901 cctacccaaa acctgccatc gtgaccgagc ttgacgggga gtatacaaag gtatggcagg
     3961 tggagcttcc tcccgggggg ggatggactg cagtatacag cggttcaggg ggagggagcc
     4021 tcgaagtacg gggaattccc ccggaacacg tagtggtggt ggatctcgat gtccagtgac
     4081 gagatggacc agaagacgca acagcgcctg gtgtctatag ccatgcggtg gtacgagcag
     4141 atgaaggagg gagcgatccc gtccatctcg ctcccgacca ggaccaagca gaatatcgag
     4201 tttgacgacg atgcagaggt atggaagtat ggtgaccgcg aaagcatacg ctcggcacac
     4261 actgcaaaga gtgcgctcca cctcctgaaa atgtcctacg tgattgcctt cctgaaaaaa
     4321 cagctccacg agagacgctc atcgacattg agagagctct attacatctc ggaagggtgg
     4381 aaacgggcca agttctccgc gcaggacgag agcaacctgc tgatagagga tctcgagatc
     4441 ctcacagacc tccagcggga ggcattccat ctcaggcccg aagaggatgg cgccaccata
     4501 ttcggcccgc tcagactaaa agagatgacg aggagagggg agcgggtgat ccattgccag
     4561 gaggacgtcg gcgaggcggg gtaccagatt ccaagtaacg tggagaatat tgatctcctt
     4621 gagcacgacg cccgctttgt cattgcgata gagaccgggg gtatgtatgc ccggctgata
     4681 gagaacgggt tcgaccagga acaccaggcg atccttgtac atttgaaagg tcagcctgcc
     4741 cgctccaccc gccggatgct ccaccggatc aacgaggcgt tcggcctgcc tgtgctggtc
     4801 tttaccgatg gcgatccctg gtcataccgg atatacgcaa gcgttgctta tggtgccatc
     4861 aagagcgccc acatgtccga gctccttgcc acgccacgtg cccagttcat cggagtgcaa
     4921 cccagcgata tccgggacta caaccttccc gcagataccc tcaccgacaa ggacattgcc
     4981 gctctgcaga gcgagatctc cgacccgagg ttctcttcag aatactggaa gtcccagatc
     5041 caactgcagc tgaaactcaa cctcaaatcc gaacagcagg catttgcagc ccgtggtctc
     5101 gactttgtga caaaggagta cctgccccgc cggctgacag agatgggtat tctctcctga
     5161 taaaaacccc atgattctca ttgagacaca tgaatgcaga ataaaaatgg ccgagccatt
     5221 ttcatagtaa ccgctcatga ctgccattcc attgaatata cacgatgaaa atcacgaagt
     5281 gattttcatt cgaaaaaaga gagggacgct cccccacacg ctccgatgtg agcctcctgt
     5341 tatctggttt ttgggaatat tttctccggt ctgaattggc attcacacca gggtgagaag
     5401 aaaattgttc ctgtattggg gaccacgcgt ccctcacaca attattctca taccatgaac
     5461 ctgaagacca gccatgcgat gacaagcatg atcgcagagt atttcaggcc ggcaaccacc
     5521 ctcgcttcac ccatctggcc ggcaaccagc cccgagaaga tgccctggat cattgcagcg
     5581 tgtgagaaga gccttgtata gaagaagagg tcaacgtttc cgatgaatga cgccgcaccc
     5641 gctcctgctg cagatgccgc ggagcctgca gtcgccatgg tcgagaggaa cgtgctcacc
     5701 aggatcagta tcacaaagac aaagaccatg aaagagatga ggacgatgat cacgtagatc
     5761 agcatgttgt tctttcgttc cgaacgaagg gcaagcacct cgtaggtatc acgggctgca
     5821 gccctgagca cctcgctcac atctccgcct gccttgctgg ccttggcgat gaggtcgata
     5881 ctcctgtcag agagcggagt gcccaggcga gcgcggaaac gctggatggc ctcgatgaat
     5941 gtcgtattcc acgacatctc gttgtcaagg cgcctgataa acggggtgag ggtgctgtat
     6001 tccccctgtg caacaaggtg aattgcattc gggagcgtca ttccgctgtc attcattccc
     6061 gcgaggtcac ggaagaaatt cgggagggct tcctcgaggt tcttcatccg ccggctctcc
     6121 cggaagtcca tgatcgcaac gggaacaatc gcaacgagga tggaaatgat gacaatatcg
     6181 tctatgaggg tcgttgtgaa gagcacgctc atcccgtagt cccttatggt aaaggctgtg
     6241 ccgatcaaaa aaaggagggc ggcaagcggc agtgtcagga tcaggatgtt gagcggtttt
     6301 tcgaggagca cctggacagg gtgcttgagc actttacgcc agcccgaccg ctggctgcgg
     6361 tgccgctcaa gtttgctggt aatttctacc tgctcccttg caaactgtcc gatctccttc
     6421 tggctgagat ccacggaata gggtttttct cccttcaata tcccccataa cccgggcctt
     6481 tttgcaggcc cgcctgccgg aggaagaggt tttcgtctgg gcgacgaagg agatacgggg
     6541 ggcacagctg gcgatgctgg cacctctgct tcactcccgc tggcagatcc agaacctttt
     6601 tttggcagca tccccttgaa agagtctttg aggcccatct caaccctccg gagtcatgga
     6661 actgatcagg atcacgaaca tgatgctccc aaaggggatc atcatgtaaa tgatgatgta
     6721 gagaaagatc ggctgcgacg actggctcag gatggccatg attgactgga ggatgataag
     6781 gaagaggggg cctgcaacca tggccgtgac atacgactcg gatatcagcc cgagcgtatc
     6841 aaggaagagc ttctgggtct ggcggttctc gagggagtac tgctctgcct tggtgcggaa
     6901 atactcggtg aggctgcttc ccgccgaaat gcaccccatc gccccctgaa ggaactcctt
     6961 catgcgcatg ctcggggtgg tcgcagagat gagccggagc gcatcgatga ggtcccgccc
     7021 aaagagatcg atctccatcc cgatgaaacg ggcctcgact gcactctcac cgtacatcgt
     7081 actgctcccc agctgccgga agatctctgc ggggggaatt cccgcggtcg acattgcggt
     7141 aatatagttg atggcatacg gaagcgtggc gtcgatgttc ctgcaacggc ctccggcgac
     7201 cacgcttggg tatatgagaa atatgaggaa tgtaataccc ccgatcaccg caaacgagat
     7261 gattatggca agtacagtgc cgatgatcag gctgtactcg ttgatcgtga gcatgaactc
     7321 cggaaccgca ccctgatact tgatcatcgc agggagattc aggagatacg tgatcagccc
     7381 gagcacgatt gatgcgacga tacctgcgat aaccgaactt aagtatgccg tcgagagata
     7441 gacctcgaac gggaccttca tgcgcgcggt gacaaggtcg ttccggagtg tggaatacgc
     7501 atccctcttc ttctccgcgt aaccccccat tatcctgaat gcgagacgtt caaacccgtt
     7561 catgctcccc catacagttc ggccctgatc ttctccatgg ttcctgcagg gtcacggtag
     7621 tactggacaa gaatattgcc aacgtccctg aagttgcgta tcttcttgat gcgcatccat
     7681 tccagcacct cctgccgcat cttgagctcc tccctcatcc ggtcctcatc ccacccccgc
     7741 gattccatga tctcctcgag gacgtaggac ttcccggaat aggtgatctc gtcggtcgcc
     7801 gggtgccacc tgaagacctc gtttgtaatg agctcgttgg tgcggggatc gatatcaagt
     7861 atctcgataa tctgcttgtt tctccggatg cgctggccac ctatccttgc ctggacctgg
     7921 actgacacga ggtcaagcgc ggagagcatg ttcctcggga cgttcagtgg cgggttttcg
     7981 agacggtgaa cgatgctggc cacggagtcg gcgtggatgg tcgagtaggt cacgtggccc
     8041 gtactcattg cctggaagag agtcagcgct tcctttccac ggacctctcc gacaaggata
     8101 tattcggggc gctgcctgag tgcggcacgg aggagctcgt acatgtcgat ctcccctttc
     8161 ccgctcgtgt caaacgaggt ccgcgtgata ctcgggatcc agttgggatg ggggagcttg
     8221 agctcgcgtg tatcctcaag agtgacaatc tttgccaggg ggggcataaa gagggagatg
     8281 gcattgagcg aggtcgtctt tccagacgcg gttccccctg caaagatgca ggacttgccg
     8341 ctctccacgg ccatccagaa gaaggcgatt gagagggggg agaatgtgcg ccactcgatg
     8401 aggtctgtgg gagtgatcgg ctcatccttg aacttcctga tcgtgtaagt ggacccgtga
     8461 gcagtcacct cagtgccgag tgtcatctgg atacgggagc cgtcactcat ggtggcatca
     8521 agcatcggtt ccgcgataga gatgtactta ccggcacgct gggcaagctt ggtcacaaac
     8581 gagtcgagtt catccgagct cttatacagg aggttcgtac gcatcgactc gtagcggttg
     8641 tggtagacga acaggttggc gtttacgcca tcgcaggaga tatcctcgat ataccggtcg
     8701 tgcatgagtg ggtctatcat cccgtttccg aggaattcct tgtacatggt gtagaggatc
     8761 ttttccctct ggaccggcga gagcgagagc ccgtagtcat ggataatgtc gtcggtaaca
     8821 tcgcggagcg tcttctttgc gatctcctcg agcacattct gggtattgat gtcaaggcgt
     8881 tcgaatatcc gttccttgat gtccctgaac agatcaacct cggcaggagt cagttttggt
     8941 tcaaggactt cgtacaggta ttcatgggtt gcgttatcat aggtgatgcg gacatacgca
     9001 tacggctcat tcacagggta tagctctgtc tgttcaatcc cgggcctcgg ccggaatgta
     9061 aggtccacga gaggaccgtg ggtcttcgga ttatactcga ccggcactct atggatgaca
     9121 tttattggat tgagccttgc aaggagtccg ccctttccgg cctcctcgtg gacctcgatt
     9181 gcggcgtcag atactttctt gagactggaa tcacgaagat cggtcttctg gagatcctta
     9241 cccgcaaccg caccgctttc tcccttgaac gagaagatat tgctgcggtc ctggatcttg
     9301 atctcctcta tctggaatgt ggcccctttt gggaggatca acccggagag gtcgttgatc
     9361 tggtcgacgg atatcactgt ctcttcaaga accggcttct tctctgacca aacggtcttt
     9421 tttacaggct caaagcgtgg agggcttcga tcggtgacgg tctctttttt ttcaatcggg
     9481 gggatttctg cgggggcgga gatatccagg gctttttttt gggagtccag accggcaagg
     9541 ggtttttcca ccacctcaac gggcggcatc tcctctccgg gttcaatgcc ggagggaacc
     9601 ttctcctgcg gcccgggaga gggggttttc tttgtgtcaa gggacaggcc gagacgcttc
     9661 atgaagccgt caaggtcttc gcgttctact ggaggaaccg atacctcatt cttctcctgc
     9721 gtcccctctt ttttgggttt atcggcaggg ccttctccct tctttcttcc aaaaaggtct
     9781 ctgaatgaaa ggcccttttt atcttctgca gtcatcgctg aattccactt ctcctggagt
     9841 tatcccacgt gaggattctc tcgcgaagta tcccccttct tataccctcc ccttcagttc
     9901 cttctgaatg gtacgtatgg caagccttat aaggccgatg ttcgcgtccg aatccgtcag
     9961 aaggcaaagg aagaggtctc cgtatggtgc gataatacac tttccctggt cgctttcaag
    10021 agttatctgg tcgggcactc ccatgcggat attctctgcc atcttcctgc cagcccgtaa
    10081 aaaatcctct gccatggctg cgacctgttc aaagtcgccg tcgccgacag actggacggg
    10141 gaacccctcg aagaagatcg agactgcacg aactcctggt tgccctgaca atcgtgcgat
    10201 aagagcatcg tcgagtcctg ccctgaccgg ttttttggca tgcgtatcct cctcgaacaa
    10261 ggacaatccc tccctcctgc agatctggat cgcatcactg gcctgaccgg gggaatacct
    10321 gtactggata taacggatac taagtcctgc ctcttcagag cgatgctgca gatgtaccac
    10381 tgcatccttt ccccgataat ccccggaatt atcgtggtaa taggcagcgt cgacccgtcc
    10441 attatccacg atgaggagac ctgccccttc gtccgtcagg atctcaactt ttcctgtaaa
    10501 aggactcgct cccccggggc ggtcctcctg gacagggtca gacgaaacac ccgcatacgt
    10561 accttcgggg agactcatgc cttataacgc tgcaacaagg cgttctttgt ttttctttaa
    10621 ttcgtaacgg attcgtccga tgtttgcctc gtttccggcc accaccgcga tcaattcatc
    10681 cgcagagagg ggggacatga ggataggtcc atgatcaagc tcgacaagca tctggttgag
    10741 ctctcctttt cccagctcct ttcccatagc ctcagatgtc cccatccccg tagatgccat
    10801 cgctccgagt gcatcaatat ccattttccc tgaagctgca ctctcaatca caaaaccatc
    10861 acgccccacc acgactgcgg cagtgactcc atcaatcttc aggaattctc ccaaaatctg
    10921 tttcagcata catgccacct acctataaag catctttgtt gatatataaa aataaaaatg
    10981 tttgctaaga tcgttcgcat atcgcacgag gggattcttc gcctgttttc acttcctgtt
    11041 cccggcaatc ccggcaccaa taaccttatg aatcgaagag tattgatcac tactgatgca
    11101 actaccccgg gggactttcc attccatgaa aaaagggctg gaattgggca ctatcctgct
    11161 gaaactcaag gatgagaatt tttccggttc ctgttcactt gtctgcgaga gccaggccat
    11221 tgacatggtc ttccaggacg gtcgcgttat gctggcaagc tgtgacacct cctgcggtga
    11281 tgcggctatg gatatcatca gccaaaagca ggactgtatc gtggatgcgt cgctctctga
    11341 ccttacccct gcccagctga gactcacgct cgagttcaat aatgggtgca gggtccgccg
    11401 ccagagaccc tccgcaggta agacccctgc ccctgcgccc gatgggtgga cgcccgtccc
    11461 gaaccaggag acgcagaaag taacttcgca atctcccccg gtcaaaaata ccgggaagat
    11521 gcccgcaatc tcctcatcgc ccgtcccggc acccgtggta atcccccgcc accagggtga
    11581 acgggaaaga ggcccggctg gcagtcccgc gttggatggc gatgaatata caatcgttga
    11641 cagggacctg gacgccctgg atgccatgga ccttgatacc atgagcgaga agatccggat
    11701 gaactgcaaa accatgattg agaaactcca cctcgaacac ctcatcgacg acagtggaaa
    11761 atgaggaaga acagccatgg ccgcagttag tttcgataca ttcattctcc ttgtgttgct
    11821 cgttctcgtc gggatagtcc tgtttctttc tcttgtgact gctatccatg tcatcagact
    11881 ctcccgcgaa atcgccttca ggcagaagag agaagagaag gccacaggca ggaatgcccc
    11941 ccctgcaggg agtgatgtga agacccaggc agacactgtt gcggctgcga cttatacacc
    12001 cgttgcagga cgcaagagag attttgatgt cactcaggat gccccggata tccccacggg
    12061 aatgaagaac ctgtgcgcac actacgacct cacgtctctt tccatctcct cacccgacgg
    12121 ccttttgatc gcttcttcag gctatcccgg cgcgataaac gatgcggcac aatacagtta
    12181 tgcattccag acaggagttc ccataaaaga gagcggagtg caggtgttcc ctgtggattt
    12241 cggcacctct cccctggtgg gaatccttcg cagtacaagc gcactccctg atacgtcggt
    12301 tgattctttg caggatgata tcgccgcgct attaaagaga tggatttgag agacgttcat
    12361 tttccgtgcg gttcattaac cttatataaa tacaggatga cattacattt tatggagggt
    12421 tgaatggtcc gagtctttac gattgcgtct ggaaagggag gaacaggaaa aactacggta
    12481 accgtcaacc tggggacgag ccttgctcaa ttcggaaaaa ataccattat aatggacgcc
    12541 gacatcggga tggcgaacat gggcctggca ctgggtctcg aggacgtccc cataaccctt
    12601 cacgaggtcc ttgcggggaa agcaaatgtc catgaggcga tatatgacgg cccggcagac
    12661 gtgaaagtgg tgccgagcgg aatctcgctc cagggatttc agcaggcaaa tcccgagaga
    12721 ctcaaggacg tactgaaaga actggtcgag gtatgcgatt ttctccttat cgatgccccc
    12781 gcgggaataa gccgggacgg ggtgatcccc cttgcggtgg ccgatgacgt gatactcgtg
    12841 gtcaaccccg agctctcctc tattgtcgat gcattgaaga ccaagatcct caccgaggtt
    12901 gtaggagggc atgtgctcgg agttattata aaccgggcag gaatcgaaaa gacggaagtg
    12961 gtgaagcaga agatggaaaa ggtgctcggt gtgaaggtaa tcggtactat cccggaagac
    13021 cccaatgtca ggaaagccgc ttcattcaag acaccagtag tgatcaagta tccactctcc
    13081 tcttcatcgg tggccatcaa gaacatagcc gccgacctgg caggaatccc tgttgagcac
    13141 cggggtgtgg agagccagga acggttcatc gagcgttttg caaaagtcct tttcaggggg
    13201 aaaaaatgag cttcgagatg atcctgctta tcatcagcag cgtggtcata ctcttcctcc
    13261 tcatcctgat aatattcatg tatatcagga tcaggcagct tatccgcgat ttcgaactgc
    13321 tggagtcgca gataatggtg acagactcgg aattcgacgc cctgatgcag aacgtggatg
    13381 agattggtca tctcaagatc tgatttcaca ggggatcaga tacctggaat ttttttccca
    13441 ggcctggttt ataaccaatt ttcaattcgg gataccattc ccctaaatta cgacaaatgg
    13501 ataaaagaaa agagattagg ctatcttagc aggacaaagc gggggactcg catacctttt
    13561 tgggtctgga aagagatgag attggggata ttggacggaa aaatatcagg attctccttg
    13621 ccggttcttg atctggaaca cgcagtgttc gtctcccttt gatttgcacg taatctccct
    13681 gcactggacc ttgatcccaa aagaaccttc aatgattcct gcaaggagac ctgccgtaca
    13741 gaagcagacc ggttttcccg tattgccaaa cgattcggac tcgaccgtat gggagagtgt
    13801 gattgtcttc gtcttggtct tctcgtccca ttcttccttg acgatattga accagcccat
    13861 ctgggagtag aaggcaaatg ccatgtcgga gagtgtcttt ggattgattt ttttctggcc
    13921 ttcttcctta atcatgtctt ccggagttcc cacaaacagg ataccgatga tctctcctgc
    13981 gggattcctg attggctcat acgccacgac atacttcttc ccaaccacat ctgcagtgcc
    14041 gtaataggtc tgccctttgt tgatcacggc attgtagacg acacttgaaa tctcggttcc
    14101 aacagcccgc ttgccatcag cgccaataac gttggtagag atacggattg ccttatcacc
    14161 ggttttctgg aagaccgtgg ccttgctgcc ccgttccttt tcaatggaat caacaacctc
    14221 gaagttatcg ttgacgacat ggtcgccgta tgcaatcttg cctccggtta ttgaaggaga
    14281 cccatatgct gcgaaacggt catgcaacag tttgagatcc ccgtcaagtt tcgtcctgga
    14341 gaggtcaaaa tagatactca tcttcttgta gtgttccacg ttctcctttc ccatgtccat
    14401 gtaggcacgg tagttgacac cgcttgccgg cgcctctccc atggtcttct tcagcgaatt
    14461 agtcatggac gtgaagatga accggggcat catgacaacc ggttcatcca taagggagat
    14521 cttcccttca aggggtttgt gctcgatgaa ctcagtgatt gctgattccc ctctgaccac
    14581 tgcctctgcc ttgaacttcc agcttggctc gacccgatcg gcaacaaact cacaatacgg
    14641 gtctccgttc gcgtggcaac tcttctcgag gcagtaccag gtcttcccgg tgattgctga
    14701 aagaatgcct tcgatccacc cctggtgaag gccacagacc ggcattttcc agtccttgag
    14761 cacctcggac tcaaacgtct gcttgcagcg aagcactatg cgcttgtcac ttgcactgac
    14821 cagttcaaag ggggaacccc aaccctgctg gtgctgaagt ttgaagacga gcatcaggaa
    14881 ctcttccggc ttcaggttca gaccgagttt ctttaagagt ttatctagcc cgactgatcc
    14941 ttgtcccaca gaacgaaaca gtttccgcat cgccttgttg gcaagcttcc tgttaaggtt
    15001 ggcctcgtac atctcataga gagccttgac catgtaattc gggttgcttg cggtccttac
    15061 cccaaaacat gtggtgaccc cctcgagcgg gttcatctgc atgtaggagg taatatcctt
    15121 gctcccattg aggatggcct caaaatcctg cttatccatt ggatccttct cctcaatcca
    15181 gatatctgat gtaagctcac ttaaaaatgt agcatgtcct tttcatgccg cgataaccac
    15241 agccttgcag aatcttcctc aattacgaga tatcgaggtc caccacctgg ttcaaaagac
    15301 caggatggaa ttcaaattca tagacccgcg tggagaggtc acgaaggtgg accacgcact
    15361 cgtaacgatc ataaagaagg cgaaaactga ccctgccttc cgcgctggta aaatcatttc
    15421 cgacaatctg gcctcccttc cggatggaga ccctctgacc gctgacgggc tttccctcct
    15481 tgacaaccct cattgcaaac acccctgcgc caccctccct ttttggtgtg acctgcggga
    15541 tgtcgcttgg gagcatgcga tcgagaatat tcctgtcaaa tatccggcac ccaaggatga
    15601 tcctttcaat gatggcacgg tcaacggggt aaaaagtgaa ctgttccgtc cccttcagga
    15661 tgtataaagc cttattccca aagagcatcc ccttgcgatc attgaatact gcaccatcgg
    15721 gttcccccgc aaagaaaagg atgtagtatc tctggtctct ctcaaccgct cgtgcaatgc
    15781 cgtacacccc ttttgaccgg gccatctgaa ggagagcctg gactgatgcc ggtccatctc
    15841 cggccgattc cgctatcagc gagtccagga tctcaatact ggtcaatgaa gatcatctcc
    15901 tgcccgtctc attgttcatc tcatctctcg cgatatcatg accgaagggg aggattgagg
    15961 ggttccatgc gattccatgg gcctcgtgtt gcaggtgctg ctccggggcc atgcataaga
    16021 acctgcacga ctcagatgta cctgtatcca tgataatccc tctggcaata ggttcagcgg
    16081 cggtgttatg tacttttctc tatgcatctt gcagggattt gaaggagcgg agagttcctt
    16141 tccgcacctg gtatcccatg cttgctgtca ccctccccat ggctgcgtgg ttttatgcct
    16201 cgtttctgat aaggggagaa tggacaatcc ccgcatattt ccttgcgttg actgcaatat
    16261 tcgcgttggt attttatctc tttgcctggc tgaacctctt tggcggggct gacgcctggg
    16321 ccctcatctt cctgtcggtg agcatccctg catttcccat cgagccgctg tccgggtatc
    16381 cccctgcagg gttctttcca ttcgcggtgc tcgtgaacgc ccttctcctg aaccttttca
    16441 ctcccctgtt actgggcctg cagaacctgc tgcacggacg ccgggctcct tttccgtaca
    16501 tgttgctggg atatcccgtc cctgcggtcg aattgccggg ggcttatggt tttatcatgg
    16561 aggatattga agagaacgag gacgggtcca tcacgaggcg gtttgtgcga ccgctggagg
    16621 ctgtaaggag gatgttttca ggtgaaaaaa ggatctatac caaggacctc cggcttcatc
    16681 ctgatgacta ttcgaaggag atggccctct tcaaactggc cggccaggta tggatctcat
    16741 acgggatccc atttattgtc cccctgactg cagggttctt atccgcgctc ttctttggag
    16801 atatcctctt tttcctgatt aaaagtgttt ctggtgtata atatgctcaa gctttcatat
    16861 gcagacggac taatcccggt ggtggtgcag gatgccggca ccagggcagt attgatggtt
    16921 gcttatgcga atgcagaggc ggtcgaactc acgaagaata ctgggtttgc tcattatttc
    16981 tcacgcagcc ggaagaagat ctggaagaaa ggagaggaga gcggccacct gcaaaaggtg
    17041 cttcgcatcc ttgttgactg cgacgaggac tgcctgcttt acgaggtgga gcaggccggt
    17101 gcggcgtgcc acatgggata ccgatcatgc tttttcagga ccatcaacgg agatattgtc
    17161 gaagagaagg tatttgatcc ggaaatggta tatgccaaaa aagggcattg aagggctttt
    17221 tcacactaca cattttttcg taggattctc atgctgggtc cccgcagtac ttcatgagaa
    17281 cctgaaggag atcacactaa tagggtttga aaaacctggt atccgcatcc attacaatgt
    17341 gtatgcagca aaatccgaaa tatcacacat gccaggatat tgatgagggg gcgtggaaat
    17401 tgggctcgag gttatcggag ccttcttttc taaaaaccac ataagtatta ttgagacaca
    17461 tgcatacaaa atgaaaatcg cggatgcgac tttcaaagta aaaaatcatg ctcccctgca
    17521 cgacagaact ttcatgtatt tccgggagta atgataatcg tggaccttat aatgacggct
    17581 gtggggcacc atacactgct gatcagagat acttccagtg acgaaaatat gcccaaacca
    17641 tgcccgaatc cacaatataa aacaaaaaag ggtgaaaatt ttgaaattag taccggatac
    17701 cagtgtagtc atcgacggtc gaatcacctc catgatacaa accggagaat ataagggtgc
    17761 atcaataatc atacc
//
LOCUS       DAOQYR010000043        17632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_3595_length_17632_cov_8.643910, whole genome shotgun sequence.
ACCESSION   DAOQYR010000043 DAOQYR010000000
VERSION     DAOQYR010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 17632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17632
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3595_length_17632_cov_8.643910"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /locus_tag="PKM87_06520"
     CDS             <1..131
                     /locus_tag="PKM87_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HNQ29758.1"
                     /translation="GRGRAALASMLQSCSVIAVVNIDAGFVAGAFAAQIANMVGER"
     gene            128..655
                     /locus_tag="PKM87_06525"
     CDS             128..655
                     /locus_tag="PKM87_06525"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide adenylyltransferase"
                     /protein_id="HNQ29759.1"
                     /translation="MKRGFYIGRFQPYHNGHQYILEKIAKTADEIIIGVGSAQLSHEP
                     ANPFTAGERVLMITRALKHIGCPLYVIPIEDIRRNALWVAHVRSMVPPFYTVYSGNPL
                     VVRLFEEEGIRVESPAMYERDSHSGTEIRKRILEDGDWESLVPDAVVQVIREIDGVRR
                     IRQIARSDGDGREPW"
     gene            662..1942
                     /locus_tag="PKM87_06530"
     CDS             662..1942
                     /locus_tag="PKM87_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29760.1"
                     /translation="MLRKVKDLFGKKEAPVVQEIRLDDIPALISDHEPVWDAELVQAA
                     SVSRKKILSTRDRLLAAVRRLTESKRDAAFHPKLEKIVQNSLPQFEKAIIFALSRPLP
                     EGADEFYQACTESLKGCVKGLAGPGRYLRNVFPEEMKEIRALIDEFGKEVNLLTPMVA
                     QSRARREKVRVILEAYSRAREMETALDGLSKEIPTLEKEIGGLRRRRDENQGTIESCD
                     RQMKDDPAFKEAGALAERSMKEMSEIGRELQGTFSTLVHVLRKAEKITHRAETEKLAR
                     DMRRVIDLVSSKEVPAAMDVEREVGAVLPIVSSMIASGEVQLKNQEERDLFFSPEKIP
                     WILSKMIREREKAGERVAIAEAQLAAHPIRVLRGKAEDDLRSIRSELKEKERQLQDLK
                     GRQKEMEADYPRLSLQMETHLSALLEGTWHIATG"
     gene            2221..2487
                     /locus_tag="PKM87_06535"
     CDS             2221..2487
                     /locus_tag="PKM87_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29761.1"
                     /translation="MPEDEYRVLEVRKGKVPCLPGKEETVEHCRFCVHSRYFRVKGEY
                     VKSPALAYCLRHRDAKEVDLSAVEAVKCGDRRGEGYRSMMSIIG"
     gene            complement(2515..2853)
                     /locus_tag="PKM87_06540"
     CDS             complement(2515..2853)
                     /locus_tag="PKM87_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein E"
                     /protein_id="HNQ29762.1"
                     /translation="MPMRVSITIDPDTMDLVDKFGKEHGLDRNRAILECIEAGYARLT
                     DGTPVSFTQTKTFEEYNDLKKAITDVKESLSNLAEEVRLIHHTIEVEWTKEIRGVPFQ
                     SKRWYEFWKS"
     gene            complement(2980..4416)
                     /locus_tag="PKM87_06545"
     CDS             complement(2980..4416)
                     /locus_tag="PKM87_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HNQ29763.1"
                     /translation="MSLMVLGTSSHAGKSTVVAALCRVLVNRGISVAPFKSQNMSLNS
                     YVTRDGCEIGIAQAMQAFAARIEPAPEMNPILLKPKGDATSQVVLMGHPYKDVPAREY
                     YLETEFLLEKAVSAARTLEERFGHLVVEGAGGAAEMNLYKRDIANILLARELQYPIIL
                     VADIERGGVFAQVYGTVRLLPEDISPLVVGIIINKFRGDPEIFSQGIEIIEEICGVPV
                     LGVLPYAGMNLPSEDSLSIAEKKSLNHPVRIAIIRLPRISNFSDFEALERDASVEYVP
                     LGSPLADYDCIILPGTKNTVDDLAEVRDSGTAEEVRRARKRGTPIIGICGGYQMLGRE
                     VIDSGIESRQGTHPGIGILDVVTRFDRYEKKTEQVTIDACPVGPVLSMMGKVSGYEIH
                     MGMTERAGAVEAFFGEGAATPDGLVFGTYIHGLFSNASAVDALLSFLYARRGISYAPR
                     IAERDPYEDLAAMFSQHVRMDLILPYFE"
     gene            4468..6120
                     /locus_tag="PKM87_06550"
     CDS             4468..6120
                     /locus_tag="PKM87_06550"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNQ29764.1"
                     /translation="MELLDPARVLLASRYLLPGESPEGMCERVAHAVGTQKKRQFLEI
                     IESLQFLPNSPTLMNAGTPTGQLSACFVLPVEDSIRQIFAVLGYMALIHKSGGGTGFS
                     FSRVRPRGDSVAGTAGVASGPLSFIRVYDEATNALRQGGKRRGANMGVLASSHPDVIE
                     FIRAKEAGGLPNFNLSVGFDGAFFACMERNLSYPLVNPRDGSVWDELEPGVLWDFISR
                     SAWRCGDPGVLFLDRINEANTVPGLGEIEATNPCGEQPLLPWESCNLGSVNLSRCVRN
                     GDIDWDVLSRTVWLAVEFLDAVIDVNRFPVPLIREHTLLTRKIGLGVMGFADLLIRMG
                     IPYQSDEALSTAERIMRFIQHESHEASRMLGEGKGSFPAIDQSVFREPMRNATVTTIA
                     PTGSLHLIAGTSSGIEPLFACAGERMIDKKAFRIMHPVLHTILRDLPRGRDLLNEVEK
                     TGSVQHLAVPEEIRELFRNAAEIDPGHHVRMQAAFQKHVDNAVSKTVNLSELTTPEDI
                     SRIFLLARELGCKGVTVYRYRSRKDQVLSRGCDTCRVDSLLL"
     gene            6217..6522
                     /gene="yciH"
                     /locus_tag="PKM87_06555"
     CDS             6217..6522
                     /gene="yciH"
                     /locus_tag="PKM87_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HNQ29765.1"
                     /translation="MNGGICPTCGLPKELCICEEVAKEQQRISVKVDRRRYGKEVTVI
                     EGLNPTDIDLEDLSKYMKGRLACGGTVKGTSIELQGNHKERVKDLLVHKGYSVDNIN"
     gene            complement(6539..6919)
                     /locus_tag="PKM87_06560"
     CDS             complement(6539..6919)
                     /locus_tag="PKM87_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon containing protein"
                     /protein_id="HNQ29766.1"
                     /translation="MPHRCVNCGREYRTNECEILKGCRECGGKKFIFVPLVEREKEAK
                     APDLSEGKEEKVQREEEPAGIARLPGHEPLESIRIVSPGTYELNIEKMASSDERVVGV
                     GKGEGSYLVDLLSMVKPKKKKSKK"
     gene            complement(6926..7291)
                     /locus_tag="PKM87_06565"
     CDS             complement(6926..7291)
                     /locus_tag="PKM87_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2073 domain-containing protein"
                     /protein_id="HNQ29767.1"
                     /translation="MMQGIQIDLISSEKLAALTSMEKIHLILDHVRKGSIIILEKGLS
                     PDEQSTLIEMTMREILPDGFNGIEIETYPSRDQSPGFLQKLLGRSAIESRLTVIGPAN
                     QLRMIKKEKDIISAWVSTR"
     gene            complement(7298..7939)
                     /locus_tag="PKM87_06570"
     CDS             complement(7298..7939)
                     /locus_tag="PKM87_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Era-like GTP-binding protein"
                     /protein_id="HNQ29768.1"
                     /translation="MNILVQTKRRLSKFLMAFFKKKKARIGIYGPPNAGKTTLANRIA
                     RDWTGEDVGLVSEVPHETRRAVRKSDISITGSNGSNITIDIVDTPGVTTKIDYKDFLE
                     YGMDKETAVIRAREATEGVAEAMHWLREDINGVMYMLDATEDPFMQVNIMMIGIIESR
                     KLPVIIVANKIDLPDASPQRIRSAFPQHPVVPISCLEGKNIEDLYEKMVEHFG"
     gene            8151..8720
                     /locus_tag="PKM87_06575"
     CDS             8151..8720
                     /locus_tag="PKM87_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNQ29769.1"
                     /translation="MKKSSDTIRFDTRVPVREVMRRNPTTIGYEGTVARAAMLMCRDE
                     VGSCIVLGRDNLPVGIVTEEDINCKVVAKDLKPSAVQASEIMSTPLITISADKYAKDA
                     AHMMIRHRVRRLPVVDDENKVIGIVTVRDILTVSTEINELMNDLVEINRLEEVEVGTC
                     SRCGQMSDDLRRTDNLMLCPSCREEELLQ"
     gene            8717..9469
                     /locus_tag="PKM87_06580"
     CDS             8717..9469
                     /locus_tag="PKM87_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNQ29770.1"
                     /translation="MKIARDFMVRVPLLKYDEKATRARQVLRDDVYRELYVHDGRRRL
                     MGCVDISGVLNVTATRSNVTIEGFIKEVSPVGPDSSIDTVAKTIRENGTDSIPVVDEG
                     GQVLGGVLLSEIFPVIITRHRLGGLVEEYMSRKVVECPADEPIHRIHTLITESGFSAF
                     PVVKKKRLVGIISRRDLLRDGRWRVSAEGASSTPVAGVMTTPVITVSPGESVQKAAEM
                     LVRHDISRLPVVLGDELIGIIDRHDVLNAIPD"
     gene            9595..10488
                     /locus_tag="PKM87_06585"
     CDS             9595..10488
                     /locus_tag="PKM87_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNQ29771.1"
                     /translation="MPGKLDRGPIEFKSRVVEQEGEIMAIATGDVVSVPQTMSIIGAV
                     ETMTRCGFRRLPVVDAGSRKIRGIVTSGDIINFLGGGDKFNLVQVKHDGNLLSAVNES
                     IRSIMTTQVTSLPDSASLRDALDIIMGKMIGGIPIIDQDEALVGIVTERDVMTVLANE
                     RVSCTVEEIMSRSLRVTEPDCPIGKVTKEMTRYRFRRLPVVSDEVLYGIITTTDIMKY
                     LGSKRVFDQLETGDIAEVMALPVRTLVSGNLYTIAPDRSVNQAAAEMVKRNAGALPVI
                     EDARLIGLVTEFDLVKALSRG"
     gene            10495..11334
                     /locus_tag="PKM87_06590"
     CDS             10495..11334
                     /locus_tag="PKM87_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNQ29772.1"
                     /translation="MQAQDIMTSPVYVVNPGEHVAYARNLMLKHKISRVPVMDAGALA
                     GILTKKDIGYRLQQSEPIWRRRPIDKIPVSILMTRDPLIISPTTTIREIAALMIHWDI
                     SGLPVVDEGEVGGIVTKSDFMESATINSLSQTVADVMEDAISVSRYHSLDHIVDIMKE
                     RNDKVVVVNNDGTLAGIITESNLAFYLYETERRESPRKDVTMLRRGESAGRKQYRHVI
                     EMAAVAEDLMSRPVITVDGNAKLTEAIEIMQANHINSVVAVEGNEIKGILKRDDIIRE
                     VAK"
     gene            11331..12107
                     /locus_tag="PKM87_06595"
     CDS             11331..12107
                     /locus_tag="PKM87_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNQ29773.1"
                     /translation="MSDDILVKDIMSKPVTISKSALITDALDRMLDEGIDPLIVVNHN
                     TVVGTVSRQSVADTLGTKRNSSISPTSIHVANTVEEDFTTVYPDQDLDVVIPLLQAYK
                     LVVVYDEDHRLVGQVSAGDVLRVMRPQGTLPDVMEPACTIDVDERVVHWRRRMIDDNL
                     HRCVVTENDQVVGIVTETDVAVAMRRFREMVEDRHQDHRIRNLLVKDIMTSPVLTVDM
                     SASIDEVVDLMHQRGISSIPVTNGGKIAGLVTRGSLIKAL"
     gene            12314..13315
                     /locus_tag="PKM87_06600"
     CDS             12314..13315
                     /locus_tag="PKM87_06600"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HNQ29774.1"
                     /translation="MGATQPVVPSDNVAELLQGMAKTGFQGRKLGESVEVWKEMLRDP
                     EVTIFFGLSGAMIPAGMQKILIELARRHYIDVVVSTGANIFHDVCEHLGVCHYLGHHH
                     VSDAGLYEKGIDRIYDVFAFEEEFRWVDRGFANFAERIAPFRGSSREFLQRFGEWLIR
                     EVPDGNSFLAACVRERIPVFVPALCDSSIGIGALIARRRGVEIDIDQIADTDEVTSLV
                     EEGRKTGVVYVGGGVPKNFIQQTQVIGSIHEKDLGGHDYAIQYTADSPHWGGLSGCTF
                     EEAISWGKESAATRHVQVHVDATIALPIVVSALIGSGVPRVGRKGAKRSKVLRQGVD"
     gene            13385..16324
                     /locus_tag="PKM87_06605"
     CDS             13385..16324
                     /locus_tag="PKM87_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ29775.1"
                     /translation="MDLPYLRHRILYVDDEPALLDICRLFLEKSGHFEVVTVNSGFLA
                     LDCLAHQHFDAVVSDYQMPGMDGIELLRRVRREGNTIPFIIFTGKGREEVVIQALNEG
                     ADHYLQKGGQPKAQFVELAHKVKTSILKKEAEAALKESEEKYRMLVEINRDIIFSISP
                     DGRVTYVSPQVLPQLGYIQKEVTGSVFSDFVHPDDLEILKQHMQDHFKDGSQVKTDQF
                     RLQNKDGRYRWFEDKSIYTRDIHGMPLLIGALRDITERRRDEETLRLTQLSVDRAAEG
                     VVWISAQGRLLYVNDQECRMMGYSREEMLQLSVWDINPFRTPESFHERWKMFREKKTW
                     VMESVHRKKNGEMIPVEVTGHYISVGEEELYYAFVRDITARKAVESALQESEEKYRLL
                     VEHSEDVIYIHQGEYFLFLNKRGQEILGYTRDQIRTIPIWEFIHPDDRTRTMEYSRKR
                     FLGEDVPSRYVARVKLKSGEVRYGEFSVNRILFQGQPATIGIVRDVTVRHAAEEALRE
                     SEEKYRILVEHTQDGVFIIQEGRLVSMNPAFTQMTGYGEEDSGVKLQDLVAPEDLERV
                     LTTYRDRLLGKPVPDSYEFRGVHKDGRKILHLKMSVGLGVFRGKPASIGTIRDVTREM
                     EERRALQETEERYRRLMEQSFDAVIIHQDGKIMVSNDRAAWIAGAKCGEDLVGRSISD
                     FIHPESHSLVQERVSRMIERPGTAMPLVEETFTKIDGSAVDVEVMATSFSYQGRPGVQ
                     VIFRDISIRKEREREMQVTNQKLNLLSGITRHDIRNKVLVASGFLSLAAQEAQNPGLR
                     AYLEKTDKALQDIRDQIQFAGEYESIGMRSPAWVNVRETIVKSFSGLDLTGIEVDITV
                     NDLEIIADHLIERVFFNLCDNSLKHGGKVSRITIRAFEERGALLLVLQDNGFGIPPTA
                     KTEIFKRGYGKNTGLGLFMVREILGITGIQIRETGSDGEGARFEVVVPAGCFRYVTSR
                     GSSKGSS"
     gene            16442..16526
                     /locus_tag="PKM87_06610"
     tRNA            16442..16526
                     /locus_tag="PKM87_06610"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16476..16478,aa:Ser,seq:gct)
     gene            16561..17013
                     /locus_tag="PKM87_06615"
     CDS             16561..17013
                     /locus_tag="PKM87_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNQ29776.1"
                     /translation="MAQEEEIRYFVRISNTDLDGTKPVLTALTGIKGVGRHTASVIAA
                     MAKVNGRDLMGKLKEEDVEKLRAAVDGYAQKVPGWMVNRPKDVYTGEARHLLGTEVST
                     TLDEDINLMRKIRSYRGIRHETGQKVRGQRTKSTGRTGATVGVKRKKE"
     gene            17031..17576
                     /locus_tag="PKM87_06620"
     CDS             17031..17576
                     /locus_tag="PKM87_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNQ29777.1"
                     /translation="MGYPGKNHKQYQTPKRPFEKTRIEEENRLMIEYGLRNKREVWKA
                     ASHLRRYRKAARDLLALMSTGTDRALFEAKKAELIGHLQRTGLLGPDADIDNVLSLKV
                     QNQLERRLQTLVHRQGLARSPKQARQMITHGHIAIGGKRVSIPGYKVSRSEETEISYY
                     RRSPVANADHAERARIARTGR"
BASE COUNT         4147 a         4562 c         5220 g         3703 t
ORIGIN      
        1 tgggcagggg cagggctgcg cttgccagca tgctccagtc gtgctcggtc attgcagtcg
       61 tcaacatcga cgcgggcttt gtggccgggg catttgcagc ccagattgcg aacatggtgg
      121 gagagagatg aagcgcgggt tttacattgg gagattccag ccgtatcaca acggtcacca
      181 gtacatcctt gagaagatcg caaagactgc agacgagatc atcatcggtg tcggcagcgc
      241 ccagctctcg cacgagcctg caaatccgtt cactgcggga gagcgggtgc tgatgatcac
      301 gcgggcgctc aagcatatcg gctgtccgct ctatgtcatc cctattgagg atatcaggcg
      361 caatgcgctc tgggttgcgc acgtgcgatc gatggtcccg ccgttttaca ccgtgtactc
      421 gggcaatcca ctggtggtcc gcctctttga agaggaggga atacgtgtcg agtcgccggc
      481 catgtacgag cgtgattcac acagcgggac cgagatcagg aaaaggatac ttgaagatgg
      541 cgattgggag agcctcgtcc cggatgcggt tgtgcaggtg attcgcgaga ttgacggggt
      601 aaggaggatc aggcagatag cgaggagcga tggggacggc agggaaccgt ggtgatatgg
      661 catgctccgc aaggtaaaag acctcttcgg caagaaggaa gcgcctgtcg tgcaggagat
      721 caggctggat gatatcccgg ccctcatctc agatcatgaa cctgtgtggg atgcggagct
      781 tgtgcaggca gcatccgtct ctaggaagaa gatcctctcg acacgggacc gtcttctggc
      841 cgctgtccgg cggcttaccg agagcaagag ggatgctgcg ttccacccga aactcgaaaa
      901 aatcgtgcag aattccctcc cccagttcga gaaagcgatc atctttgcac tgagccgccc
      961 actgccagag ggtgcagatg agttctacca ggcctgcacg gaatccctga aagggtgcgt
     1021 gaaagggctt gccgggccgg gacgatatct ccgaaatgtc tttcctgaag agatgaaaga
     1081 gatccgcgca cttatcgacg agttcggaaa agaggtcaat ctccttaccc ctatggtggc
     1141 gcagtcaagg gcaaggcgcg agaaggtccg cgtcatcctt gaggcatatt cacgtgcccg
     1201 cgagatggag actgcactgg acgggctttc aaaggagatc ccgacccttg aaaaggagat
     1261 cggggggctc agaagaaggc gcgacgagaa ccagggcacc attgaatcct gtgatcgcca
     1321 gatgaaggat gatcctgcat tcaaggaagc cggcgctctg gcggaacgtt cgatgaaaga
     1381 gatgtccgaa atcgggcggg agctccaggg aacattttcg acgcttgttc acgtgctacg
     1441 aaaagccgaa aagatcaccc accgggcaga gacggagaag cttgcccggg acatgagaag
     1501 ggtgatcgat ttagtctcgt caaaagaggt ccccgctgct atggatgtgg aacgggaggt
     1561 tggtgccgtc ctgccgatcg tgtccagcat gattgcatca ggggaagtac agttgaagaa
     1621 ccaggaagag agggacctct tcttttcccc tgaaaagatc ccctggattc tctcgaaaat
     1681 gattcgagag agggaaaagg caggtgagag ggttgcaatc gcagaagcac aactggcagc
     1741 tcacccaatc cgggtgctgc gcgggaaggc agaggacgat ttgagatcga tccggtccga
     1801 actcaaggag aaggagcggc aactacagga tctcaaggga agacagaagg agatggaggc
     1861 cgattacccc aggctttccc tgcagatgga aacgcatctt tctgccctgc tcgagggcac
     1921 ctggcatatt gccacgggat gaccgctgca gtttcagcag cagggcatat cgttggtata
     1981 aacctcttat gtatccggag aattgctggc caggaccaac cctttgtgcc tggtgaagcc
     2041 ccctttttaa agttgtttcc ggttcaatga aagttttcca ccatgagtga cgaggactta
     2101 tgacatgcat tgcgcgattg aattacaatc caatagttgt caagccccgt tgcacccgtg
     2161 tttgtccggg actggagggt agatatagat cggaagtgaa cactggaaag gagaattctg
     2221 atgccggaag acgaataccg ggtccttgag gtgcgcaagg ggaaggttcc ctgccttccc
     2281 gggaaggagg agacggtcga gcattgcagg ttctgcgttc attccaggta tttccgggtg
     2341 aaaggggagt acgtgaagtc accagccctc gcatactgcc tccggcaccg cgacgcgaaa
     2401 gaggttgacc tcagcgcagt tgaagccgtg aaatgcggtg acaggagggg ggagggctac
     2461 cggagcatga tgagcatcat cgggtgacga agggatcatc ccggctggta ggggtcagct
     2521 tttccagaac tcgtaccacc gcttgctctg gaacgggaca ccccgtatct cctttgtcca
     2581 ttccacctcg atggtatggt gtatcagcct tacctcttct gcaaggttcg agaggctctc
     2641 cttcacatcg gtaatagcct ttttgaggtc attatactcc tcaaatgtct tcgtctgggt
     2701 gaagctgaca ggtgtcccgt ctgtaagcct ggcatatcca gcctctatgc attcgagaat
     2761 ggcgcgattt ctgtcaaggc cgtgttcctt gccgaattta tcgacaaggt ccatggtatc
     2821 cggatcgatg gtgattgata cccgcatggg catggtagta cctcctccac acttctttcg
     2881 cgagtgagtt tgtgtctcct cactcaaata tctccctgtc tgcagccggg agatccaaac
     2941 agcccggtgc caggtaaaat ccagcgcatt ctgtgaaaat cactcgaaat aaggcaggat
     3001 gaggtccatt cgcacgtgtt gagagaacat ggctgccaaa tcctcgtaag ggtcacgctc
     3061 cgctatccgt ggtgcataag agatcccccg tcttgcataa agaaaactaa gcagcgcatc
     3121 aaccgcggac gcattcgaga acaggccatg tatgtatgtg ccgaagacca ggccatcggg
     3181 ggtagccgct ccctcaccaa aaaacgcttc gactgcccct gcccgctctg tcattcccat
     3241 gtggatctcg taacccgata cctttcccat catggagagc accggtccga ccggacatgc
     3301 atcgatggtg acctgctcgg tcttcttctc atagcggtcg aaccgagtca ccacgtcaag
     3361 gataccgatt cccgggtggg ttccctggcg agactctatc cccgaatcga taacctcccg
     3421 cccgagcatc tggtaccccc cgcatatacc aataattgga gtgccgcgct tccgcgcccg
     3481 ccgtacctcc tctgctgtcc ctgaatcccg gacctctgcg agatcatcga cggtattctt
     3541 ggtccccggc aggatgatgc agtcatagtc ggcaaggggc gatcccaggg ggacatattc
     3601 cacactcgcg tctctctcaa gcgcctcaaa gtcgctgaaa ttggagatcc gggggagtct
     3661 gatgatggca attctgacgg ggtgattaag cgactttttc tccgcgatcg agagcgagtc
     3721 ctcgctcggg aggttcatgc ccgcgtatgg cagcaccccc agcacaggga ccccgcaaat
     3781 ctcctcgatg atctctattc cctgcgagaa gatctccggg tcgccccgga acttgttgat
     3841 aatgatgccc accaccagcg gggagatatc ttctgggagg agcctgactg ttccatacac
     3901 ctgtgcaaac acaccgcccc tctcaatatc ggccacgaga atgatggggt attgcaattc
     3961 cctcgcgagg agaatgtttg cgatgtccct cttgtagagg ttcatctctg ctgcaccgcc
     4021 cgctccctcc accaccaggt ggccgaagcg ctcttccagg gtacgggcgg cagacactgc
     4081 tttttcaagg agaaattccg tctccaggta atactccctg gcagggacgt ccttgtatgg
     4141 gtggcccatc agcaccacct gggatgttgc atcacccttt ggcttgagga ggattgggtt
     4201 catctccgga gccggctcaa tcctggcagc aaaagcctgc atagcctgtg caattcctat
     4261 ctcgcacccg tcacgcgtga cataggagtt caggctcatg ttctgcgact tgaacggtgc
     4321 cacgctgatc cccctgttga caagcacccg gcagagtgcc gccaccaccg tgctctttcc
     4381 tgcgtgagag gaggtgccaa gcaccatcag agacatgtgc ataaaatatg gatggattta
     4441 aataaagagc attcctctca ctgcgttatg gaactcttgg acccggcgag agtgttgctg
     4501 gcaagccgtt acctgcttcc gggggagtcg cccgaaggga tgtgcgagag ggtggcgcac
     4561 gcggtgggga cccaaaaaaa gcgtcaattc ctcgagatca ttgagagttt acaattttta
     4621 ccgaattccc ccacactcat gaatgcggga accccaacag gccagctctc cgcgtgcttt
     4681 gtcctgccgg tcgaggattc catccgccag atcttcgcgg ttcttgggta catggcgctc
     4741 atccacaaga gcggcggcgg tactggtttc tccttttcac gcgtgcggcc ccggggggat
     4801 tccgttgccg ggacggcggg ggtggcgagc ggtcccctct cattcatccg cgtgtatgac
     4861 gaagccacaa acgcgctcag gcagggtgga aagcgccgtg gcgcgaacat gggtgtcctt
     4921 gcgagcagcc accctgacgt aatcgagttc atccgggcaa aggaggctgg cgggctgcca
     4981 aattttaacc tctcggtcgg attcgacggt gcgttcttcg cctgcatgga gagaaatctc
     5041 agctatccgt tggtgaaccc ccgggacggg tcggtatggg acgagctgga accaggggtt
     5101 ctctgggact ttatatcacg ctcggcatgg aggtgcggcg atccaggcgt gctctttctt
     5161 gaccggatca acgaggcgaa cacggtcccg ggcctcggag agatcgaggc cacgaatccc
     5221 tgcggggaac aaccgctcct cccatgggag agctgcaacc tgggcagcgt caacctctca
     5281 cgatgcgtgc ggaatggtga tattgactgg gatgtcctct ccaggacagt gtggctggca
     5341 gtggagttcc tggatgcggt cattgatgtg aaccggttcc cggttcctct catcagggaa
     5401 cacacgctcc tgaccaggaa gatcgggctc ggcgtgatgg gatttgcaga cctgctgatc
     5461 cggatgggaa ttccctacca gtcggatgag gccctctcga cggctgaacg gatcatgcgc
     5521 ttcatacagc acgagtccca tgaggcatca cgcatgctcg gggaggggaa aggttcgttc
     5581 cctgcaatag accagagcgt gttccgtgag ccaatgagaa atgccaccgt gaccaccatc
     5641 gcgcccacgg ggtcactgca cctgatcgcc ggcacaagca gcgggatcga gccgctcttc
     5701 gcatgcgccg gagagaggat gatcgacaaa aaagcctttc gcatcatgca ccctgtcctt
     5761 catacaatcc tccgcgatct tccccgggga agggatctcc tgaatgaagt ggaaaagacg
     5821 gggagtgtcc agcacctcgc agtaccggag gaaatccgcg aacttttcag gaacgccgca
     5881 gagatcgatc ccgggcatca tgtgaggatg caggccgcct tccagaagca cgttgacaat
     5941 gccgtctcca agaccgtgaa cctgtccgaa cttaccaccc ccgaggacat ctcccgcatt
     6001 ttcctcctcg ccagggaact gggctgcaag ggagtcacag tctatcgcta ccgcagccgg
     6061 aaggaccagg tcctgtcacg ggggtgcgat acctgcaggg tggactcgct tctcctgtga
     6121 gacttcgaaa aagacccgga tttccatcgt tatacgtgca ttgcgcgatt ctggggatgc
     6181 caacaaatat ataagcagaa cttgaaggta cttgtaatga atggtgggat atgtccgacg
     6241 tgtggactgc cgaaagagtt atgcatctgt gaagaagtcg caaaagaaca gcagcgtatc
     6301 agcgtgaagg tcgacaggag acgttacggg aaagaagtca ccgtgatcga gggcttgaac
     6361 cccacggaca tcgacctgga agacctctcc aagtacatga aagggagact tgcgtgcggc
     6421 ggaacggtga agggcacctc catcgaactg cagggcaatc acaaggagag ggtcaaggac
     6481 ctgctggtgc acaagggata cagcgtggat aatatcaact gaaaatgaaa taagaaacct
     6541 atttcttgct ctttttcttt tttggcttga ccattgaaag gaggtccacc aggtagctgc
     6601 cctccccttt cccgactccg acgacacgct cgtcggagct ggccatcttc tcgatattca
     6661 gctcgtatgt gccaggagag acgatgcgga tgctctccag tggttcgtgg cccgggagac
     6721 gcgcaatacc tgctggctcc tcctcacgct gcaccttctc ctcctttcct tctgaaagat
     6781 ccggggcttt tgcctccttc tccctctcga cgaggggcac aaagatgaac ttcttgccac
     6841 cgcattccct gcagcctttc aggatctcgc actcattggt gcggtattcc cgcccgcagt
     6901 tgacgcaccg gtgtggcatg agaactcacc gcgtggagac ccaggcgctg atgatgtcct
     6961 tctctttttt tatcatccgc agctggtttg ccgggccaat gacggtcaaa cgcgactcaa
     7021 tcgctgatct tcccagcagt ttctggagaa aaccgggact ctggtccctg gaggggtagg
     7081 tctctatctc tatcccgttg aacccgtccg gaaggatctc gcgcatggtc atctcgatca
     7141 gggtgctctg ctcgtcagga gagaggccct tctctaagat gataatggag cccttccgca
     7201 cgtggtcgag gatgagatgg atcttctcca tcgaagtcag cgccgcgagt ttctcggatg
     7261 atatcagatc tatctggatt ccctgcatga tccaccccta tccaaaatgc tccaccatct
     7321 tttcgtacag gtcctcgatg ttcttcccct caaggcagga tatcggtacc acgggatgct
     7381 ggggaaacgc actgcggata cgctgcggcg acgcgtcggg caggtcgatc ttgtttgcga
     7441 caatgatcac cggcagctta cggctctcga taatcccgat catcatgatg ttgacctgca
     7501 tgaagggatc ctctgtcgca tccagcatgt acatgacacc gttgatatcc tcacggagcc
     7561 agtgcattgc ttccgcgacc ccttcagtgg cttccctcgc gcgtatcacc gcagtctctt
     7621 tgtccatgcc gtattcaaga aagtccttgt agtcgatctt ggtggtgacc ccgggagtat
     7681 cgacgatatc gatggtgatg ttgctcccgt ttgatccggt gatgctgata tcgctctttc
     7741 gcaccgcccg ccgggtttca tgggggacct cgcttaccag gcccacatcc tccccggtcc
     7801 agtccctggc aatacggttg gcgagtgtgg tctttcctgc atttggcggg ccgtatattc
     7861 caattctggc ctttttcttc ttgaagaaag ccattaaaaa ctttgagaga cgtctcttgg
     7921 tctggaccag gatattcatt gtacacctcg tcatgaccag gcagggaatg tgcagtatat
     7981 cccccgggtc atgcgaatcc gttactatcc ttttaggggg ggggtggtta ttagatttat
     8041 tatccttgcg tttctgggcg cactggaact tgcagaaagt atatagactc catttgccgt
     8101 attggtaact gcaataaacc cacttgtgtc aacaacatgg aggtgatcga ttgaagaaat
     8161 catccgatac catccgcttc gatacccgtg taccagtacg ggaggtcatg cggcgcaacc
     8221 caaccaccat cggatatgaa gggacggttg cccgtgcggc tatgctcatg tgccgggatg
     8281 aggtgggaag ctgcattgtg cttggcaggg ataacctccc cgtcgggatc gtgaccgagg
     8341 aggatatcaa ctgcaaggtg gtggcaaagg atctcaagcc aagtgccgtc caggccagcg
     8401 agatcatgag cactccgctc atcaccatca gcgcagacaa gtatgcgaag gatgcagcgc
     8461 acatgatgat caggcaccgg gtccggcggc tccctgtggt tgacgacgag aacaaggtaa
     8521 ttgggattgt aacagttcgt gatattctca cggtatccac agagattaat gagctgatga
     8581 acgacctggt ggagatcaac cgcctggaag aggtggaggt tgggacctgc agcaggtgcg
     8641 gccagatgtc agatgatctc aggcggacag acaacctcat gctctgtccg tcttgccgtg
     8701 aggaggaact gctacaatga aaattgcccg ggacttcatg gtaagggtac ccctcctgaa
     8761 atatgacgag aaagctaccc gggcgaggca ggtgctccgt gacgatgtgt atcgcgaact
     8821 ctatgtgcat gacgggagac gccggctcat ggggtgcgtg gacatatcag gggtactgaa
     8881 cgtcacggca acgcgttcta acgtgacaat agagggcttt atcaaggagg tcagtcctgt
     8941 tggcccggat tcgtccatag ataccgttgc aaaaacgata cgggagaacg ggaccgacag
     9001 tataccggta gttgatgaag gaggccaggt acttggaggg gtgctccttt cagaaatttt
     9061 ccctgtgatc atcacccggc acagactcgg gggacttgtg gaagagtaca tgtcaaggaa
     9121 ggtcgtggaa tgcccggccg acgagcccat tcaccggatc cacaccctga tcactgaaag
     9181 cgggttttcc gcatttccgg tggtgaagaa gaagcgcctg gtgggcataa tctcccgccg
     9241 ggacctgctg agagacgggc ggtggagggt gtctgcagag ggggcatcga gtacccctgt
     9301 tgcaggagtg atgaccacac ccgtgatcac tgtctctccc ggggagagcg tgcagaaggc
     9361 cgcagagatg ctggtcaggc atgacatcag ccgtctcccc gtggttctcg gagacgaact
     9421 gatcggcatc atcgaccgcc acgatgtgct gaatgcgatt ccggattaaa ttggggaagg
     9481 acgagcaatc ccggagttca tcatggaccg ggatattcga gcaatacatt ttcgggggca
     9541 ggaatggtac atcacattga caggaactat aaacagagcg ataaacttct gaaaatgcca
     9601 ggaaaactcg atcgcgggcc gatcgaattc aaatcgaggg tcgtcgagca ggagggggag
     9661 atcatggcga tcgcgaccgg tgacgtggta tccgtgccgc agaccatgag catcatcggg
     9721 gcggtggaga caatgacccg gtgcgggttc cggaggctgc ccgtggtgga tgccggctcg
     9781 aggaaaatcc gtgggattgt cacttcgggg gatattatca atttcctggg aggtggggac
     9841 aagttcaacc ttgtccaggt gaagcatgac gggaacctgc tctcggccgt caacgagagc
     9901 atccgaagca tcatgacgac ccaggtgaca tcgcttcccg actccgcatc gctccgggat
     9961 gccctcgata tcatcatggg aaagatgata gggggcatcc ccatcatcga tcaggatgag
    10021 gcgctggtgg gcatcgtgac agagcgcgat gtcatgaccg tgcttgcaaa cgagagggta
    10081 tcctgcaccg tggaagagat catgagccgg tccctcaggg tcaccgaacc ggactgcccg
    10141 atcgggaaag tcacgaagga gatgaccagg taccggttcc gcaggctgcc ggtggtgagc
    10201 gacgaggtgc tctatggtat catcacgacg acggacatca tgaagtacct cggcagcaag
    10261 cgggtattcg atcagctgga gacgggggac atcgccgagg tgatggcatt gccggtccgg
    10321 acactggtct ccgggaacct ctatactatt gcccctgacc ggagtgtgaa ccaggctgcc
    10381 gcagaaatgg tgaagaggaa cgcgggcgcg cttccggtaa tcgaagatgc gaggctgatt
    10441 gggctcgtga ccgagtttga cctggtcaaa gccctctcaa gggggtgatt cgtaatgcag
    10501 gcacaagata tcatgacatc tccggtctat gtggtcaacc caggagaaca cgtcgcgtac
    10561 gcacgcaatc tgatgctgaa gcacaagatc tccagggtgc cggtcatgga tgcaggagca
    10621 cttgcgggca tcctcaccaa gaaggacatc gggtaccggc tgcaacagag cgaacctatc
    10681 tggaggcgtc ggccaatcga taaaatccct gtgagtatcc tgatgacccg cgatcccctc
    10741 attatctccc ccactactac gattcgcgag attgcagccc tgatgatcca ttgggatatc
    10801 agcggccttc cagtggtaga tgaaggagag gtggggggaa ttgttacgaa gtcggacttc
    10861 atggagtctg caacgatcaa ctctctctca cagactgttg ctgatgtcat ggaagatgcc
    10921 atctctgtca gcaggtacca ctctcttgac catattgtcg acatcatgaa ggaaaggaat
    10981 gataaggtag tcgtggtcaa taatgatggc acactggcgg ggatcatcac ggaaagcaac
    11041 cttgcattct acctctacga gacagaaagg agggaatcgc caagaaaaga cgtgacgatg
    11101 ctgcgccgtg gggaatcggc cgggaggaag cagtaccggc atgtgatcga gatggcggct
    11161 gtcgcggaag acctgatgtc ccgccctgtc attaccgttg acgggaatgc gaaactgaca
    11221 gaagccatcg agataatgca ggcaaaccac atcaacagcg tggtagcagt ggaaggcaac
    11281 gagatcaaag gaattttaaa aagagacgat attatacggg aagtggcaaa atgagcgacg
    11341 atattctggt aaaggacatc atgtccaagc cggtcaccat ctccaagtcc gcactcatca
    11401 ccgatgccct ggacaggatg cttgacgaag gcattgaccc tctcatcgtg gtgaaccaca
    11461 acacggtagt aggcactgtt tcacggcaga gcgtggcaga cacgctcggg acgaagagga
    11521 actcctcaat ctcgcccacc tcgatacatg tggcaaacac ggtcgaggag gacttcacca
    11581 cggtctaccc cgaccaggac ctcgacgtgg tcattcccct cctccaggcc tacaagcttg
    11641 tggtggtcta cgatgaggat caccgcctcg tggggcaggt ctcggctgga gatgtgctcc
    11701 gggtgatgcg gccacagggc accctccctg atgtgatgga gcctgcatgc accatcgatg
    11761 tcgacgagcg cgtggtgcac tggaggaggc ggatgatcga tgacaacctt caccgctgtg
    11821 tcgtaactga gaacgatcaa gtggtgggaa ttgtgaccga gacagatgtg gccgtcgcca
    11881 tgcggaggtt ccgcgagatg gtggaggaca ggcaccagga ccaccgcatc cggaaccttc
    11941 tcgtcaagga tatcatgaca agccctgtgc tcactgtcga tatgagcgca agcattgacg
    12001 aggtggtcga cctcatgcat cagagaggca tcagttcgat acccgtgacg aacggaggga
    12061 agattgcggg gcttgtcacc agggggtctc tgataaaggc cctttaacca cctttttctt
    12121 tcgattgttt tgttcttcag gggttcttgg ggtatccgtt ctcggtgcac cgtcgctctg
    12181 cacggcgcac atttcttatc aattgcccgt ccatctctat gcaatggcag ggaaaaagga
    12241 agagcacctt gggcaacccg tgggaatcga atcgattatg ggcggacccc tgaagaaagt
    12301 ggaacggtac gggatgggtg caacacagcc tgtcgttcct tccgataacg ttgccgagct
    12361 cctccagggg atggcaaaga caggattcca ggggaggaaa ctcggcgagt cggtggaggt
    12421 ctggaaagag atgctgcgtg acccggaggt taccatcttc tttggcctct cgggggccat
    12481 gatcccggca ggcatgcaaa agattctcat cgaacttgcg aggcgccatt atatcgatgt
    12541 ggtggtctct acgggtgcga atattttcca cgatgtctgc gaacacctgg gtgtctgcca
    12601 ctacctgggc caccaccacg tcagcgacgc cgggctgtat gagaagggaa tcgatcgcat
    12661 ctacgacgtc tttgcgtttg aagaggagtt ccgatgggtg gacagggggt ttgcgaactt
    12721 cgctgagagg attgcgccgt tcagggggtc ttcaagggaa tttctgcaga ggtttggaga
    12781 gtggcttatc agggaagtgc cggacggcaa ctcgttcctc gcagcatgcg tgcgggagag
    12841 gatacctgtt tttgtcccgg ctctctgcga ttcatcgatc gggatcggtg cactcattgc
    12901 gaggcgccga ggcgtcgaga tcgacatcga ccagattgcc gatacggacg aggtcacgtc
    12961 ccttgtggaa gagggcagaa agactggggt ggtctacgtg ggtggcgggg tcccgaagaa
    13021 cttcatccag cagacccagg ttatcggttc tatccacgag aaggaccttg gcggccatga
    13081 ttatgcgatc cagtacaccg ccgactcacc gcactggggg ggtctgtcag gctgcacctt
    13141 cgaagaggcc atcagctggg ggaaggagtc agcggcgacc aggcacgtcc aggtgcacgt
    13201 ggatgccacg attgcgctcc caatcgtcgt atccgcgctc atcgggagcg gcgtgccaag
    13261 ggtcggcagg aagggtgcaa agaggagcaa ggtactcagg cagggggtcg attagtaaaa
    13321 ttccaagatg acctgatcag gtactttttt atctcgaaat cgtcacacgc tttcccacac
    13381 aacaatggac ctcccatatc tccgtcaccg tattctctat gtcgacgatg aacccgccct
    13441 tctggacatc tgcaggttat tcctggaaaa atcaggacat tttgaagttg ttacagtaaa
    13501 ctcggggttt cttgcccttg attgcctggc ccatcagcat tttgatgcgg tagtctctga
    13561 ctaccagatg cctggcatgg atggtattga actcctccgc agggtcaggc gggagggaaa
    13621 cacgatccct ttcattatct tcacaggcaa aggcagggag gaggtggtca tccaggcgtt
    13681 gaacgagggg gctgaccact atctgcagaa aggaggccag ccgaaggcac agtttgtcga
    13741 gcttgcccac aaggtgaaga catctatctt aaaaaaagag gccgaagctg cgctgaaaga
    13801 gagcgaagag aagtaccgga tgctcgtcga gatcaacagg gatatcatat tctctatatc
    13861 tcccgatggc cgtgtcacct atgtcagtcc tcaggtcctc ccgcagctgg gatatatcca
    13921 gaaagaggtg accggaagtg ttttttcaga ttttgtccat cccgatgacc ttgagatcct
    13981 caaacagcat atgcaggatc atttcaagga tggcagtcag gtcaaaacgg accagtttcg
    14041 actccagaat aaagatggca gataccgttg gttcgaagat aaaagcatct atacacggga
    14101 tatccatggc atgcctttac tcatcggggc gcttcgcgat attactgaaa gaagaaggga
    14161 tgaagagacc cttcggctga cacagctctc tgttgatcgg gcagctgagg gagttgtctg
    14221 gatctcggcg caaggcaggc tgttgtatgt gaatgaccag gaatgcagga tgatgggata
    14281 ttccagggaa gagatgctgc agttgtctgt ctgggatatc aatcctttcc gaactccaga
    14341 atcctttcac gagcgctgga agatgttccg tgagaaaaag acctgggtca tggagagcgt
    14401 gcaccggaag aagaacggcg agatgatccc ggtggaagtg acgggccatt acatatcagt
    14461 cggtgaggag gagctgtact atgcgtttgt cagggatatc accgcacgga aagcggttga
    14521 atccgcactc caggagagcg aggagaaata ccggttgctg gtagaacaca gcgaagatgt
    14581 gatctacatt caccagggag agtattttct ctttctcaat aaacgggggc aggagatcct
    14641 gggatatacc agggatcaga tacgcacgat cccgatatgg gagttcattc accctgatga
    14701 tcgcacacgg accatggaat attccaggaa aaggttcctc ggagaggatg tcccttcacg
    14761 ttacgtggcc agggtgaaac tgaagtcggg tgaagtccgt tacggcgaat tttcggtcaa
    14821 cagaattctt tttcaaggtc agccggcgac gattggaatc gtcagggacg tcacggtcag
    14881 gcatgcggcc gaagaagcgt tgcgggagag cgaggagaaa tacaggatac tggtggaaca
    14941 tacccaggat ggggtattca tcatccagga gggacgactg gtgtccatga accctgcatt
    15001 cacccagatg acagggtacg gcgaggagga ttcaggggtg aagttacagg acctggttgc
    15061 acccgaagac cttgagaggg tcctgactac ctaccgggac aggctcctgg gcaaacctgt
    15121 ccccgattcc tacgaatttc gcggcgtcca caaggatgga aggaaaatac ttcatctgaa
    15181 aatgtcggtt ggtcttggcg tattccgtgg gaaacccgca agcatcggga caattcgcga
    15241 tgtgaccagg gaaatggagg aaaggcgggc attgcaggag accgaggagc gttaccgccg
    15301 tctcatggaa cagtcattcg atgcggtaat cattcaccag gatggaaaga tcatggtctc
    15361 caatgaccgg gctgcctgga ttgcaggcgc aaagtgcggc gaagacctcg tggggcgatc
    15421 catatcggat tttatccatc ccgagtcgca ttcgctcgtg caggagcggg tgtccaggat
    15481 gattgagaga ccgggaactg ccatgccact ggtggaggag acgtttacga aaatagatgg
    15541 gagtgccgta gatgtcgagg ttatggcgac ctcgttcagc taccagggga gacctggggt
    15601 ccaggtgata ttcagggata taagcatccg aaaggagagg gagcgggaga tgcaggtgac
    15661 gaaccagaaa ctcaaccttc tttcaggtat cacccgccac gatattcgga ataaagtact
    15721 tgttgcttct ggattccttt cactggctgc acaggaagcc cagaatccgg gtctccgggc
    15781 atatctggaa aaaaccgata aagccctgca ggatatcagg gaccagatac aattcgccgg
    15841 tgaatatgaa tctataggga tgagaagtcc ggcatgggtc aatgtcaggg aaacgattgt
    15901 gaagtctttt tcagggctgg atctcacagg cattgaggtg gatattaccg taaatgacct
    15961 ggagataatc gcggatcacc ttatcgagcg ggtctttttc aacctgtgtg acaattcgct
    16021 gaaacacggt ggaaaggtga gccggataac aataagggca tttgaggaaa gaggagcatt
    16081 gctattagtt ctccaagaca acgggttcgg gattcctcca acagcgaaga ctgaaatatt
    16141 taaaagggga tacgggaaga atacaggcct cggtctcttc atggtgcgag agatccttgg
    16201 tattacgggg attcaaatca gggaaacggg ttcggatgga gagggagcaa ggtttgaagt
    16261 ggttgttccg gcagggtgtt tcagatatgt gacatccaga ggcagttcaa aggggtcctc
    16321 ataagggccg gcaacgagat cggagaaagg gtttttataa cgtgtgcacc taataataac
    16381 gcaccattca tggagcagca ccttaccgat gctgcgagta tcgaaagatg cgagattctc
    16441 tgttgaggta gccaagccag gtatggcgca ggtttgctaa acctgtgtcc ccttgggact
    16501 cgagggttca aatccctccc tcagcgctcc agaagaattc tcacaaaaga gaggtattgg
    16561 atggctcagg aagaagagat acgatatttc gtacggatca gcaacaccga cctggacggc
    16621 accaagccgg tcctcaccgc cctgacaggc ataaagggcg tgggaaggca tactgcctcc
    16681 gtgattgccg ccatggcaaa ggtaaatggc cgggacctga tgggcaagct caaggaagag
    16741 gacgtggaga agctccgcgc agccgtggac gggtatgccc agaaagtccc aggctggatg
    16801 gtcaaccgcc ccaaggatgt atacaccggc gaggcgcgcc acctcctcgg gaccgaggtc
    16861 tccaccacgc tcgacgagga tatcaacctc atgcgaaaga ttcgcagtta caggggtatc
    16921 cggcacgaga ccggccagaa ggtgcggggc cagaggacca agtccaccgg tcgcacaggt
    16981 gccactgtgg gcgtgaagag gaagaaggag taaccagcag gtgaatgtgc atgggatacc
    17041 cgggtaagaa ccacaagcag taccagaccc caaagcggcc cttcgagaag acccgcatcg
    17101 aggaagagaa ccgcctgatg atcgagtacg gcctccggaa caagagggag gtatggaagg
    17161 ccgcaagcca cctccgccgg taccgcaagg cagcacgtga cctgctggca ctcatgtcca
    17221 caggtacaga cagggcgctt ttcgaagcaa agaaggcgga actgatcggg cacctccagc
    17281 gtaccggtct ccttggcccc gacgcggaca tcgacaacgt cctctccctc aaggtccaga
    17341 accagctcga gaggcgcctg cagaccctgg tgcaccgcca gggcctggca cggtccccaa
    17401 agcaggcccg ccagatgatc acgcatggac acatcgccat cggcggcaag cgcgtgagca
    17461 tccccggata caaggtttcg aggtcggaag agaccgaaat ctcgtattac cgcaggtcgc
    17521 ctgttgcgaa tgccgatcat gccgagaggg cacgcatcgc caggacaggg aggtaaggaa
    17581 tggcaggcga gaaggaaaaa tggggaattg cgcacatatt cgcgtcattc aa
//
LOCUS       DAOQYR010000044        17594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_3605_length_17594_cov_9.242545, whole genome shotgun sequence.
ACCESSION   DAOQYR010000044 DAOQYR010000000
VERSION     DAOQYR010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 17594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17594
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3605_length_17594_cov_9.242545"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PKM87_06625"
     CDS             complement(<1..292)
                     /locus_tag="PKM87_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29778.1"
                     /translation="MDILLDAVRKLKELSQHSGCEDGNIVLQVNPDCTVCQFERGACM
                     EAVFGGRVAEFVTFEPTRATTRLGFLFDATLEKPRHRAAACALINVMAGFLCI"
     gene            complement(293..910)
                     /locus_tag="PKM87_06630"
     CDS             complement(293..910)
                     /locus_tag="PKM87_06630"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNQ29779.1"
                     /translation="MSWHDVTRPLTGRVLVYPGDHVPSMVQEDRGRYLLSSLHLSTHS
                     GTHIDAPSHYIPGGAPVDAIPPSLLIGRCRVIEIQGRDTEIGAGMIRPFLEGVERLLI
                     KTWFSREDAFREDYPGLSHEAARIIAEAGVGCVGIDSPSIEPFAGDGRVHRTLLSQGI
                     AIVELLDLVCIEEGDYWMVALPLRLEGLDGSPCRVLLQDTDSMVR"
     gene            complement(915..1565)
                     /locus_tag="PKM87_06635"
     CDS             complement(915..1565)
                     /locus_tag="PKM87_06635"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNQ29780.1"
                     /translation="MREDPREAERNAMVETQIAARGVRDPRVLRAMRMVPRHLFVPAG
                     YEGAAYRDGPLPIGHGQTISQPYIVAVMTELLDISPGDRVLEIGAGSGYQAAILATLG
                     ASVITIERIPEVAALAQENLKKAGIHGVRVVVADGSEGYPAEAPYQAILVTAATPRVP
                     EPLVSQIAEGGRLVAPVGGRDIQELVRLVKKEGRVESTFFGGVVFVPLLGRHGWEV"
     gene            complement(1594..2709)
                     /locus_tag="PKM87_06640"
     CDS             complement(1594..2709)
                     /locus_tag="PKM87_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNQ29781.1"
                     /translation="MPEDIGRMEDLSTTDRERLTQTGLEVEMKNRSGSFFQVDQEVKQ
                     VAALQEGIEVLPIQEALERYGWLEDYSWKLVRKDKDQYTRFVADQPDPRGFVVIARKG
                     SRTVFPLQTCLFLAKTPVQHVHNIIIAEEGAELHLISGCASASYTKTGSHIGVSEFYV
                     GRDARITTTMIHNWGDEISVFPRSSARVEENGVFLSNYVCMQPVRKIEMSPLADLAGK
                     NSIARFSSIVVCTPGSHMDLGSVARLSAEGASAELLTRAITTGGTIISRGMIEGKTRH
                     TKGHIECKGLILKDGTIHAIPEIKGDVVDTELSHEAAVGKIAKDEIEYLMSRGLDEDV
                     ATSTIIRGFLDVKIAGLPESLQAQIDAAIDSAEKSGF"
     gene            complement(2694..3425)
                     /locus_tag="PKM87_06645"
     CDS             complement(2694..3425)
                     /locus_tag="PKM87_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ29782.1"
                     /translation="MLNIEDLHVKIGEKEVLHDINLHIKEGETHVLMGPNGSGKTTLL
                     RAIMGFGGLDVTDGHILFKGKDVTRMPIHERAKLGMGMLFQRPPTIAGLKLGKLLAVT
                     SGDRVGMVSEYSRFLRMDTFMDRAINLGFSGGEIKRSEVLQLMVQQPDFVMLDEPESG
                     VDLENISLIGTAIARLLEKDVHIVNRKKSGLVITHTGYILDYLDADFGHVMCDGVFRC
                     HGNPREILRVIKTSGYKECLACQKI"
     gene            complement(3528..4643)
                     /locus_tag="PKM87_06650"
     CDS             complement(3528..4643)
                     /locus_tag="PKM87_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29783.1"
                     /translation="MRPTLAIALLCLLFAAISAPGCLEGLPWIGAPVVYPKIVPVEGG
                     TMPVPPVYTFPFQDRVVTVNIQPDAAVYSGAKAADRSARIYDQSIPDEEWRAGIYRAL
                     MSDPSQEGFFSALVSRFHHERDAYRMDQDEYAELLAVFVQSIPYESQDETSPRFPVEM
                     YVEGIGDCDDKSLLLAALLGREGYRTALLYFEPERHMAVGVGCPGPGYQGTGFAYIET
                     TNVSLLGIPPRELSGGTTLSSLPEVIPIGDGSLCYTRCSETEELWKAVEETGRQIALL
                     EADIRIRESALMEKRDQLDAEKASMDRMRSHGNLVGYNMRVPAYNAQVEEYNKMRSGL
                     MEVVERFNHIAGIHNYLVAHPHDRKGAYAWLSALPEW"
     gene            complement(4654..5442)
                     /locus_tag="PKM87_06655"
     CDS             complement(4654..5442)
                     /locus_tag="PKM87_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HNQ29784.1"
                     /translation="MTGIRGLLIDLDGVVYTGGMQVPGANEAISWLESEGYPFRFVSN
                     TTRKSRRSLSQRLNGMGISIPPSLIFTPAIAAAALVRRRGISKVSLLTTGDVHHDFEE
                     AGLQVVREGAEVVVVGDAGDAFTYQSMNAAFRLILGGVPLIALEKDRYWMDTDGLSLS
                     AGPFVAALEYATGTEAELAGKPSAAFFRMALDSMGLTPGECAMIGDDAESDVGGAMNA
                     GMSGVLVKTGKFRADAVSRLERPPTAILPSIAALPGYLQQQADR"
     gene            complement(5439..5681)
                     /locus_tag="PKM87_06660"
     CDS             complement(5439..5681)
                     /locus_tag="PKM87_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29785.1"
                     /translation="MRAAFEHSLCTVKVKSLHREPLHYQPPARARGGTRMQQRGDERP
                     AGYTVMKSGRAGLLALHRRTRTGGNATRATHSRGCM"
     gene            5704..6774
                     /locus_tag="PKM87_06665"
     CDS             5704..6774
                     /locus_tag="PKM87_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNQ29786.1"
                     /translation="MTPPLAFETASRLWRDRIVEAPDYSVIRNDRLFVAGMSGAPVLE
                     SEYRDIQRFKSILLAQHRETPLEELFPGRTIETPEGPVYCITRRHAVRIPEGARESVR
                     KQLEGDLTLVFGIGRQKERDLKRRGYRTIADLLQHRRFREPAVNCLNVLREGSAAEVL
                     SLVSRWHPVSHPRCLCTAGLYRAEDFLFLDLETLGIYQRPVILSGLAFMEGGDLVTCQ
                     YLVRNMEEELPALLATRNHLAAGKVLVTYNGRSFDVPYLVERYAMYGEDCGVCNPHYD
                     LLHPSRRRWRDTFPDCRLSTLEQRLFSVHRQQDVPSMMVPEFYETFLTTQNPGPLVPV
                     VEHNCQDLVSLARLLCLFLEEN"
     gene            6778..>8756
                     /locus_tag="PKM87_06670"
     CDS             6778..>8756
                     /locus_tag="PKM87_06670"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNQ29787.1"
                     /translation="MGIGDFLRAISEDQDLSPGLAHIEEIPARCAEYGSLDRTLPLPI
                     HRHLESRQIRLYTHQCEAIAASRANESVILTTSTASGKTLAFAIPIFERLIEDPDATF
                     LLVYPTKALTNDQLLAFQSLEKATGIAAHSAVYDGDTETGRRPRIRQTARVLLTNPHE
                     LHHILSWHHQWSRIFRGLRGVVLDEAHRYRGVYGSHVALLMRRLRRVARHYGSEPSFF
                     LSTATIANPGEFASRLCGLPFREIAQDGSPHGKRTFLFYNPYHSWPALRSVHWDTALI
                     FSRCMDHGMQTLCFTGARKSAELVAFRASEMNPGKGGIAAYRAGYLPEERRKIEQGLK
                     SGTLRGVVSTNALEVGIDIGSLGGVILSGFPGTMMAAWQQAGRAGRSLGESLAALVAF
                     ANPLDQYFMRHPQEFFGAAHEHAIVDLGNPYILSGQVLCAGAELPIDCERDQEFFGPS
                     LPDIIAAHASESLLGKTRRGWIYSGSRRASDLVAMDGSSRETFRVLSQGRVIETMDRS
                     QAFREAHPGAILLHQGEQYRVDSCDLAAKTVHATPVDVDYYTRPLKSVNIRVTGIRDW
                     RSLPGAPLTFGDVEVTEQFTAYKLLRSETVIGIEPLDLPPLQFSTRALWVEVPPDAVE
                     AVGQQGGDLPGGLHAMEHAMIAMMPFHVLCDRWDIG"
     assembly_gap    8757..8856
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8921..9718)
                     /locus_tag="PKM87_06675"
     CDS             complement(8921..9718)
                     /locus_tag="PKM87_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HNQ29788.1"
                     /translation="MTNPYLGREDAPIQPETWSLLDSAMVEAAKSVLAGRRLLHIEGP
                     YGLGLKAVPLEDCQVSECMAVSQFVPVHLISTTFTVGIRDLLAFEQNGLPFSAEAAAE
                     AAIECARNEDTLIFEGGMGSPGLLELEGTGALKLSSWSTVGKAADDIIQAMIRLDEAG
                     YHGPYSLALAPSRYNLLFRRYLQGQSTELEHIRAIVTDGVYKAPALKSGGVLLASGRR
                     YASIVLGQDMTVGFIGPIGDSLEFSITESLALLVREPSSICVLKDKE"
     gene            complement(9729..10070)
                     /locus_tag="PKM87_06680"
     CDS             complement(9729..10070)
                     /locus_tag="PKM87_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNQ29789.1"
                     /translation="MNSDRKLSHSELVRAIRLMIADEYEAIQVYTQLAESIDNTLAKE
                     VLLDIADEERVHAGEFLRLLQELDPDEMKFYAEGAAEVEAEIAKISPGGTSPATAEKK
                     GGATTIGSMKE"
     gene            complement(10226..10705)
                     /locus_tag="PKM87_06685"
     CDS             complement(10226..10705)
                     /locus_tag="PKM87_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthase"
                     /protein_id="HNQ29790.1"
                     /translation="MRRVVATGTFDILHPGHLYYLEESGRLGDELYVIVARDRNVRHK
                     PKPVLPEEQRARMVAALKPVDHAVLGDLHDMFRPIEEIRPAVITFGFNQHFDEEKIRA
                     ELGKRGLSVELVRIGSYGGDRYCSSSEIVRQVLARFSSGKEEMHEQENREPPYLGSH"
     gene            complement(10702..11073)
                     /locus_tag="PKM87_06690"
     CDS             complement(10702..11073)
                     /locus_tag="PKM87_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HNQ29791.1"
                     /translation="MQIRGISTDLLDLLLEVGKDRHPYEFAGVIMEEEGILQELFLLP
                     GTVSSEESASLFYDMMPLDTHVAGSAHSHPNGVLFPSNADLSFFPRVGRYHLIVGFPY
                     SREDWRCYRANGEPVALEVVG"
     gene            complement(11174..12586)
                     /locus_tag="PKM87_06695"
     CDS             complement(11174..12586)
                     /locus_tag="PKM87_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNQ29792.1"
                     /translation="MRILLPTGRATLEVVREAAAGIDARVVVTGEIASFLSPARLAEL
                     LSEEKYDMAIVSGMCTASFSSVEAESGVPVYLGPRHAADLDLVLPLLPGVRLSRTIPA
                     DEFLAGERRKKAELRIASAEQESDFLYSVRGVKIGGGSRMKVLAEIMDAHRVPDLRAR
                     VEALFSLGADIVDLGFGFDATFDQVRSAFSALEDIDGPLSVDTQDPALIAAALPRADM
                     VLSLQEENIPLVGSLVAGAGAVAVVVPGRAGLRENLAMARAAGVECLVADPLLPPAGS
                     GLVDSLARFRGWDVPLFFGAGNVVELLDADSVGANALLCAMAAEIGASVIFTSEHSDK
                     TQGAVREMRRATEMMVLSRDRPYPKDLGLDLLVIKEKRRRREPPLEYRTFVQAGPVPA
                     EIEYDPHGNFRIGIEGAEVVAVIRDRAYRGTSWAEVFFAILAGEEVSLLDHAAYLGKE
                     LYKAELAIRFGRSFEQDGPF"
     gene            12629..13297
                     /locus_tag="PKM87_06700"
     CDS             12629..13297
                     /locus_tag="PKM87_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNQ29793.1"
                     /translation="MLNCDLHIHTSFSRDGESSVEEIVRRAELVGLDAIAITDHDTVE
                     GALHALTVPTRLVIIPGIEVSTRQGHLLVLGVTTPIPRGMEVTETIRRARQLGGLVIL
                     PHPYHIWRHGVARRMRSPLSEVDAIEVFNSRYIVGSANRKAARIARRLKKPCVGGSDA
                     HNARFVGYGRTLVDAAPDTASILDAIRAGRTVATGRMTPLRTYTRQSLSNTWKKFTRG
                     MQPR"
     gene            13261..14094
                     /gene="truA"
                     /locus_tag="PKM87_06705"
     CDS             13261..14094
                     /gene="truA"
                     /locus_tag="PKM87_06705"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNQ29794.1"
                     /translation="MEEVHTGNAAQVRLAFRCGYLGEGFFGSQVQPVLRTVEGEFIRA
                     CLNLGLFSSHRGARFLAAGRTDRGVHARGQVFAFSTVHPDRAVSALNRRLPPDFWTTG
                     YAVVEPGFHPRHHAVSRTYRYYFGEPDLDAEAMDRAARIFCGVHDFSLFARVEGRDPV
                     RKVLTSRVFLDQGLCVYEVSAESFLWHMVRYMASTLLMAGLGADGDEVILSRLAGDGR
                     ARLSPAPPEGLVLWDVAYAFPFVPVSAGEKALALLESRGAYHRCMGRVIDTLRDTTND
                     M"
     gene            14688..14921
                     /locus_tag="PKM87_06710"
     CDS             14688..14921
                     /locus_tag="PKM87_06710"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HNQ29795.1"
                     /translation="MPGFDGTGPSGRGPMTGWGRGRCMAYGVPAAAQGQAQVAAPFPP
                     GYAPPAYYGLGRGGIPRGCGRGFGGGRRRRCFW"
     gene            14972..15205
                     /locus_tag="PKM87_06715"
     CDS             14972..15205
                     /locus_tag="PKM87_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29796.1"
                     /translation="MTEHTKDNQGVSLGLPSSSQTPRTHGTKSQVTSIPLRTALRIAR
                     EQQRSKSSRRKIFREEPERYLDIVHLMKDPGSE"
     gene            15311..15688
                     /locus_tag="PKM87_06720"
     CDS             15311..15688
                     /locus_tag="PKM87_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HNQ29797.1"
                     /translation="MDEYIPAACAPRRRGRPRTQRVIGNMFSSRCYSPQCWPHEERAV
                     VTILPEEVELLRLVDLEGMEQEEAAAALGVSRRTAWRDLHEARRKVADALTTGKSLQI
                     GGCSRASRGICPRKCPRFPGNSP"
     gene            15922..17550
                     /gene="atwA"
                     /locus_tag="PKM87_06725"
     CDS             15922..17550
                     /gene="atwA"
                     /locus_tag="PKM87_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl coenzyme M reductase system, component
                     A2"
                     /protein_id="HNQ29798.1"
                     /translation="MKHPFISVQNLSMSFSGKKVLDNLSFDIPEGEIIGIIGRSGAGK
                     SVLMHLLRGVDEPPTEGSIVYHLSRCPKCSYIGLRRELSKPCPRCGEQLFAIDVDFWE
                     PENEQLKSLVMRRTAIMFQRTFALYGDDRVIENVLRALDDVGYSQDGAVNRAADLLDQ
                     VRLSHRMMHIARDLSGGEKQRVVLARQLAKEPCLLFADEPTGTLDPDTARLVHQMLRS
                     AAETTNMGIVVTSHFSRVIQDIATRAMLLEEGRIEKIGAPAEVINHFLRDFHEEENIS
                     EVELGEEILVARDVAKRYMSPDRGLIRAVNGVTFKVNRGEIFGLIGRSGAGKTTLSRI
                     IAGIIEPTAGEMNFLVGDDWVDMTKPGIDQRGRAKSYIGLLHQEYDLYPHRTVLDNLT
                     DAIGLEFPKELAMRKAHITLSMAGFPQSKSATILSQYPDQLSEGERHRVALAQVLIRE
                     PSLVILDEPTGTMDPLTKRDVKHSILHARQEMDQTFILVSHDMEFVQEVCDRVAWMKG
                     GKVVALGSTEEVLAEMPAEDRGMQGLAGQPSPAL"
BASE COUNT         3314 a         5606 c         5180 g         3394 t
ORIGIN      
        1 aaatgcagag gaacccggcc atcacgttga tgagggcgca tgccgccgcc cggtgtctcg
       61 gcttttcgag tgttgcgtca aagagaaaac cgagcctcgt tgttgccctg gtgggctcga
      121 acgtgacgaa ttccgcgacc cgaccgccaa ataccgcttc catgcaggca ccgcgctcaa
      181 actggcagac ggtgcaatcc gggttcacct ggaggacgat gtttccgtcc tcgcatcccg
      241 agtgctggga gagttctttc aacttcctga ctgcgtccag aaggatatcc attcacctca
      301 ccattgagtc cgtgtcctgg agcagcaccc ggcagggtga accgtcgagc ccttcgagcc
      361 tgaggggcag tgccaccatc cagtaatcgc cctcctctat acacacaagg tcgagaagtt
      421 caacgatcgc aataccctgg gagaggaggg tcctgtgcac cctcccatcc cccgcaaagg
      481 gctcgattga cggggagtcg atccccacgc acccgactcc ggcctccgcg atgatccggg
      541 cagcctcgtg agagagcccg gggtagtcct ccctgaacgc gtcttccctg gagaaccagg
      601 tcttgatgag gagccgctcc accccttcga ggaagggcct gatcattccc gccccgatct
      661 ccgtatccct cccctgtatc tcgatgaccc tgcaccggcc gatcaacagg gagggaggga
      721 tcgcatcgac gggagcgcca ccagggatat aatgcgacgg tgcatcgatg tgcgtgccgg
      781 aatgggtgga gaggtgcagg ctggagagga gatacctccc cctgtcctcc tggaccatcg
      841 agggaacgtg gtccccgggg tacacgagca ccctcccggt cagggggcgg gtcacatcgt
      901 gccaggacat cgtttcacac ctcccatcca tgcctgccga ggagtggcac gaagaccacg
      961 ccgccaaaaa atgtactctc cacccgcccc tcttttttga caaggcggac cagttcctgg
     1021 atgtcgcgtc cgcccaccgg ggccaccagc ctccccccct ccgcaatctg tgagacgagg
     1081 ggctccggca cccggggcgt tgcggcggtg acaaggattg cctggtaggg tgcctcagcg
     1141 gggtagccct ccgacccgtc cgcaaccacc acgcggacgc cgtggatccc cgccttcttc
     1201 aggttctcct gtgcgagcgc tgccacttcc gggatacgct cgatggtaat gaccgacgcc
     1261 ccgagcgtcg cgagtatcgc tgcctggtac ccgctgcccg ccccgatttc gagcacgcgg
     1321 tcgcccgggg agatgtcaag aagctcggtc atcactgcca cgatataagg ctgggagatg
     1381 gtctggccat gcccgatggg aagaggaccg tccctgtagg ccgcaccctc gtatcctgca
     1441 gggacaaaga ggtgccttgg gaccatgcgc atggcccgaa ggacgcgggg atcgcggaca
     1501 ccccgggccg cgatctgggt ctccaccatt gcattcctct ctgcttccct tggatcttcc
     1561 cgcatttatc tcccccttca cgcgatcccc gggtcagaac ccgctcttct ccgcggagtc
     1621 tatcgctgca tcgatctggg cctggaggga ctccgggagg cccgcgatct tcacgtccag
     1681 gaacccccgg atgatggtcg acgtggccac gtcctcgtcg agtccgcggg acatcaggta
     1741 ctcgatctcg tcctttgcga tctttcccac cgcagcctcg tgtgagagct cggtgtcgac
     1801 cacgtccccc ttgatctcgg gaatggcatg gatggtcccg tccttgagga tgagcccctt
     1861 gcactcgatg tgccccttgg tgtgccttgt cttcccctcg atcatccccc gcgagatgat
     1921 ggtacccccc gtagtaatcg cgcgggtgag cagctcggcc gaggcgcctt ccgcggagag
     1981 ccgtgccacc gacccgaggt ccatgtggga cccgggtgtg cagacgacga tgctcgagaa
     2041 cctggcgatt gagttcttcc ccgccaggtc tgccagggga gacatctcga tcttgcggac
     2101 cggctgcatg cagacgtagt tcgagaggaa gaccccgttc tcctccaccc tcgcagaact
     2161 tcgcgggaac acgctgatct cgtcacccca gttgtggatc atggtcgtgg tgatcctcgc
     2221 gtcacggccc acgtagaact ccgagacccc gatgtgcgag ccggtcttcg tgtaggatgc
     2281 gctcgcacac ccggatatga ggtgcagctc cgcgccctcc tctgcgatga tgatgttgtg
     2341 cacgtgctgt acgggggtct ttgccaggaa gaggcaggtc tggaggggaa atacagtccg
     2401 gcttcccttg cgcgcgatga ccacgaatcc cctcggatcc ggctggtccg caacgaacct
     2461 cgtgtactgg tccttgtcct tcctgacgag cttccacgag tagtcctcca gccagccgta
     2521 ccgttcgagc gcttcctgga tgggaaggac ctctatcccc tcttgaagcg ctgccacctg
     2581 cttgacctcc tggtcaacct ggaagaagct cccggaccgg tttttcatct cgacctcgag
     2641 gccggtctgg gtcaggcgct cccggtccgt cgtagagagg tcctccatcc tgcctatatc
     2701 ttctggcatg ccaggcactc cttgtatccc gaagtcttga tcacacggag gatctccctt
     2761 gggttcccat ggcagcggaa cacaccgtcg cacatgacat ggccaaaatc cgcgtccagg
     2821 tagtccagga tgtatccggt atgggtgatc acgaggccgc tcttcttcct gttcacgata
     2881 tgcacgtcct tttcaagcag cctggcgatg gcggtcccga tgaggctgat gttctcgagg
     2941 tcgacaccgc tctcgggctc gtcgagcatc acgaagtcag gctgctggac catcagctgc
     3001 aacacctcgc tcctcttgat ctccccgccc gagaacccga ggttgatggc ccggtccatg
     3061 aaggtgtcca tgcgcaggaa ccggctgtac tcgctgacca tccccacacg gtccccggac
     3121 gtgacagcga gcagcttccc aagcttgagg ccggcgatgg tgggaggccg ctggaacagc
     3181 atgcccatcc caagtttcgc ccgctcatgg atgggcatcc ttgtcacgtc cttgcccttg
     3241 aagaggatgt gcccgtccgt cacgtcaagc cccccgaacc ccatgatcgc ccggagcagg
     3301 gtggtctttc cggacccgtt cgggcccatc agcacatggg tctccccctc cttgatgtgg
     3361 aggttgatat cgtggagtac ctccttttct cctattttta catgcagatc ctcgatattc
     3421 agcatggtat ctctccaaaa ttttgagttg cattactatc tcagaggcca agaattaaac
     3481 ggttgtgacc atgctccccc ggctcccggg accgcccgcc acacccgtca ccattccggg
     3541 agggcagaga gccatgcata cgcccctttc cggtcgtgcg ggtgggccac gaggtagttg
     3601 tgtatcccgg caatgtggtt gaaccgctcc accacctcca tcagccctga cctcatcttg
     3661 ttatactcct ccacctgtgc gttgtacgcg gggactcgca tgttatatcc caccaggttc
     3721 ccatgtgatc gcatgcggtc catggacgct ttctccgcat ccagctggtc ccttttctcc
     3781 atcagtgcac tctccctgat ccggatatct gcctcaagaa gagcgatctg ccggcccgtc
     3841 tcttcaaccg ccttccaaag ttcctctgtc tcgctgcagc gtgtatagca gagggacccg
     3901 tccccgatgg ggatcacttc tggcagggag gagagggtgg tgccaccaga gagttcccgc
     3961 ggagggatac ccagcaggct cacgttggtg gtctcgatgt aggcgaatcc cgtaccctgg
     4021 taccctgggc ccgggcagcc cacccccacg gccatgtgcc gctccggctc gaagtacagg
     4081 agcgcggtcc ggtatccttc gcggccaaga agggccgcaa ggagcaggct cttgtcatcg
     4141 cagtccccga taccctccac gtacatctcc accgggaaac gcgggcttgt ctcgtcctgg
     4201 ctctcgtagg ggatcgactg gacgaacacc gccaggagtt ctgcatattc gtcctggtcc
     4261 atgcggtaag cgtcccgctc gtgatgaaaa cgggagacaa gcgccgagaa gaacccttcc
     4321 tgggatgggt ccgacataag cgcccggtaa atcccggctc tccactcctc atcggggatg
     4381 gactggtcat agatccgggc gctcctgtct gcggcctttg cgccggaata cactgcagca
     4441 tccggctgaa tgttcacggt caccacacgg tcctggaacg gaaacgtgta caccggcggg
     4501 accggcatgg tacctccctc aaccggaacg atcttcggat agacgacagg agccccgatc
     4561 cacgggagac cttccaggca ccctggtgca gaaattgccg caaagaggag acagaggaga
     4621 gcgatggcga gtgttggccg catggctcac gcttcacctg tccgcctgct gctgcaggta
     4681 tcccggaagt gccgcaatcg aggggaggat cgcggtcggc ggcctctcga gccgcgagac
     4741 cgcgtcggcg cggaacttcc cggtcttcac gaggaccccc gacatgcccg cattcatcgc
     4801 accccccaca tcgctctccg cgtcgtcgcc gatcatggcg cactccccag gtgtgagccc
     4861 catggagtca agggccatgc ggaagaaggc tgccgagggt ttccccgcaa gctcggcctc
     4921 tgtcccggtc gcatactcaa gcgcggcgac aaacggcccc gccgagagcg aaaggccgtc
     4981 cgtatccatc cagtagcggt ccttctcgag cgcaatcaaa gggactcccc cgagtatgag
     5041 gcgaaacgcc gcattcatcg attgataagt gaaggcatcc cctgcatcgc ccaccaccac
     5101 cacctccgcc ccttcccgga ccacctgaag ccctgcctcc tcgaagtcat ggtgcacgtc
     5161 gccggtggtg aggagagaca ctttcgatat tcccctccga cgaaccagtg ccgcggcggc
     5221 aatggcgggg gtgaagatga gtgagggcgg gatggaaatc cccatgccgt tgagcctctg
     5281 cgagaggctt ctccttgact tccgtgtggt gttggagacg aacctgaacg ggtatccttc
     5341 actctccagc caggagatag cctcattggc accgggcacc tgcatgccgc cggtgtagac
     5401 cacgccatcg agatcgatca ggagcccgcg gatgcccgtc acatgcaccc ccgtgaatgc
     5461 gtcgcgcggg tggcatttcc accggtgcgg gtgcgccggt gaagagcgag aagaccggcc
     5521 cggccgcttt tcatcacggt gtatcctgcc ggcctttcgt ctcccctctg ctgcatgcgt
     5581 gttccgcccc tcgcccgcgc gggtggctgg taatggagtg gttcgcggtg gagactcttt
     5641 actttcacgg tgcacagtga atgctcaaat gcagcccgca agatactcca ccctgagaat
     5701 cagatgaccc cgcccctggc gtttgagact gccagcagac tctggaggga ccgcatcgtc
     5761 gaggcccccg actactccgt catcaggaat gaccgcctgt tcgtggcagg gatgtccgga
     5821 gcgccggtcc tcgaatccga ataccgcgat atccagcgct tcaagtcaat actccttgcg
     5881 caacacaggg agaccccgct cgaggagctg ttccccggtc gcactatcga gaccccggaa
     5941 gggccagtgt attgcatcac acggaggcac gcagtccgga tccctgaggg cgcccgggag
     6001 agtgtccgga agcagcttga aggcgacctc acgctggtgt ttggaatagg ccgacagaag
     6061 gagagggatc tgaaacggcg gggataccgg accattgccg acctcctgca gcaccggcgg
     6121 ttcagggagc cggcggttaa ttgtctcaat gtcctgcgcg aaggaagcgc ggcagaggtg
     6181 ctctccctcg tttcgcgctg gcaccctgta tcccatcccc gctgcctctg cacggccggg
     6241 ctctaccggg cggaggattt cctctttctc gacctggaga cactcggcat ctaccagcgc
     6301 ccggtgatcc tgtcaggcct tgccttcatg gagggaggcg atcttgtcac ctgccagtac
     6361 ctggtaagga acatggagga ggagctcccg gccctccttg caaccaggaa ccatctcgcc
     6421 gcaggaaaag tgctggtcac gtacaatggc aggagtttcg atgtccccta cctcgtggag
     6481 cggtatgcga tgtacgggga agactgcggg gtgtgcaacc cccattacga cctccttcat
     6541 ccttcaaggc ggcgctggcg cgatacgttt ccggactgcc gcctctcgac cctggagcag
     6601 aggctcttct cagtccaccg tcaacaggat gtgccgtcga tgatggtccc cgagttctac
     6661 gagacgttcc tcacaacgca gaatcccggc ccgcttgtcc cggtcgtgga gcacaactgc
     6721 caggacctgg tctcgctggc ccggctcttg tgcctctttt tggaggagaa ctaaaaaatg
     6781 ggcatcggtg atttcctccg cgcgatctcg gaagaccagg atctctcacc ggggcttgcg
     6841 cacatcgagg agatcccggc acggtgcgcg gagtatggat ctcttgaccg cacactcccc
     6901 cttccaatcc acaggcacct cgaatccaga cagatcaggc tttatacgca ccagtgcgag
     6961 gctatcgcgg cgtcccgcgc caatgagagt gtgatcctta cgacctctac tgcgtccgga
     7021 aagaccctcg ctttcgccat tcccatattc gagcggctca tagaggaccc cgacgcgact
     7081 ttcctcctcg tctacccgac aaaggccctc acgaatgacc agctcctcgc cttccagtcc
     7141 cttgagaagg cgacggggat cgccgcgcat tccgccgtgt acgacgggga caccgagaca
     7201 gggaggaggc cacgcatccg ccagacggcc cgggtgctcc tgacgaaccc gcacgagctc
     7261 caccatatcc tctcctggca ccaccagtgg agccgcatct tccggggcct ccgcggggtg
     7321 gtcctcgacg aggctcaccg ctacagggga gtctatggct cgcacgtcgc gctcctcatg
     7381 cggcgcctcc gcagggtcgc gcgacactat ggatcggagc cttcattctt cctctcgacc
     7441 gccacgattg caaacccggg ggagttcgcc tccaggctct gcgggcttcc cttcagggag
     7501 atcgcgcagg acggctcccc ccacgggaaa cggacatttc tcttctacaa cccgtaccac
     7561 tcatggccgg cgctgcgctc tgtccactgg gacactgcac tcatcttctc acggtgcatg
     7621 gaccacggta tgcagactct ctgcttcacg ggagcgagaa aatctgcgga actcgtggcg
     7681 ttccgggcga gtgagatgaa tcccgggaag ggcggcatcg cggcctaccg ggccgggtac
     7741 cttccggaag agaggagaaa gatcgagcaa ggactcaaaa gcgggaccct tcggggtgtg
     7801 gtttccacaa atgcactcga ggtgggaatc gacatcggtt cgctcggggg ggtgatcctc
     7861 tcagggttcc ctgggacgat gatggccgcc tggcagcagg cagggcgggc gggccgctcc
     7921 ctcggggagt cccttgccgc gctggtggcc tttgcaaacc ccctcgacca gtacttcatg
     7981 cggcacccgc aggagttctt cggcgccgcc cacgagcacg caatcgtcga cctcgggaac
     8041 ccctacatcc tctccgggca ggtcctctgc gccggggccg agctccccat agactgcgag
     8101 cgggaccagg agttcttcgg cccatccctc ccggatatca tcgcagccca tgccagtgag
     8161 tcgctgctcg gaaagaccag gagggggtgg atctactccg ggagcaggcg cgcatctgac
     8221 ctcgtggcga tggatggcag ttcccgcgag accttcaggg tgctctcgca gggcagggta
     8281 atcgaaacaa tggaccggtc ccaggcattc cgcgaggccc accccggcgc gatcctcctc
     8341 caccaggggg agcagtaccg ggtggactcg tgtgaccttg cggcaaagac cgtccatgcg
     8401 acccccgtgg atgtcgatta ctatacccgc ccgttgaaat cggtgaacat ccgggtcacg
     8461 ggtatccggg actggcgatc gttgccgggt gctccgctca ccttcggcga cgtggaggtg
     8521 accgagcagt tcactgccta caagcttctc aggtccgaga ctgtcatagg gatcgagccg
     8581 ctcgatctcc ccccgctgca gttctccacc cgcgcactct gggtcgaggt gcccccggat
     8641 gcggtggaag cagtcggtca gcagggtgga gacctcccgg gcggtctcca cgccatggag
     8701 catgccatga tcgcgatgat gcccttccat gtcctctgcg accggtggga catcggnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcag cgctccagct cctctcgctc
     8881 gtatccgggg gtaaatgaaa gggaaaaagg gggatgaaga ttattccttg tccttcagga
     8941 cgcagattga ggacggctct ctcacgagga gtgcgaggct ctctgtgatg gagaactcca
     9001 ggctgtcccc aatcgggccg atgaacccga cggtcatgtc ctggccgagc acgattgacg
     9061 cgtagcgcct ccccgaggcc agcaggactc ctccgctctt gagggcaggg gccttgtaca
     9121 cgccgtctgt gacgattgcc cggatgtgtt cgagttccgt gctctgcccc tgcaggtagc
     9181 ggcggaagag gaggttgtac cgtgagggcg cgagcgccag gctgtacggg ccgtgatacc
     9241 ctgcctcgtc gaggcggatc attgcctgga tgatgtcatc ggcagctttt cccaccgtgc
     9301 tccacgacga gagcttgagt gcgccggtcc cctccaactc aaggaggccg gggctcccca
     9361 tgccaccctc gaatatgagc gtgtcctcgt ttcgtgcgca ctcgatggcc gcctcggctg
     9421 cagcctctgc agagaaggga aggccgttct gctcaaatgc aagcaggtcc ctgatcccca
     9481 cagtgaacgt cgtgctgatc aggtgcaccg gcacgaactg gctgaccgcc atgcactcgc
     9541 tcacctggca gtcctcgagc gggactgcct tgagaccaag cccgtagggc ccctcgatgt
     9601 ggaggagcct ccggccggca aggacgctct ttgcagcctc gaccatggcc gaatcgagca
     9661 gggaccaggt ctcaggctgt atgggagcat cctccctccc aaggtaaggg tttgtcatga
     9721 tcgtcctctc actccttcat ggacccgatg gtggtcgccc cacccttctt ctctgccgtt
     9781 gccggggatg tgccacctgg ggagatcttt gcaatctcgg cctcgacctc ggcggcgcct
     9841 tccgcgtaga acttcatttc gtcagggtcc agctcctgga gcagccgcag gaactcccct
     9901 gcgtgaacgc gctcttcgtc cgcaatatcc aggagtacct ccttcgcaag ggtgttgtca
     9961 attgactctg caagctgggt gtagacctgg atggcttcat actcgtcggc gatcatcagc
    10021 cggatcgccc tgacaagctc gctgtgggag agcttccggt cactgttcat tccagaaaat
    10081 gcagtcgcaa attcaggcat acctcatcaa tcctcatgta acaagggcct gtttaaacga
    10141 gacccctcgt tagccttatt tcaccttcca cgttaatatg gttccctctc cggaaccgtg
    10201 ccggaaggga caacggccga acccttcaat gactgcccag ataaggaggc tctctgtttt
    10261 cctgttcatg catctcctcc ttcccggagg agaaccgggc cagcacctgc cggacaattt
    10321 cactcgagct gcaatagcgg tccccgccat aactccctat ccggaccagt tcgacagaca
    10381 gtccccgttt gccaagctct gcccgtatct tctcctcgtc aaagtgctgg ttgaacccga
    10441 acgtgatgac cgcggggcgt atctcctcga ttggcctgaa catgtcatga agatccccga
    10501 gaactgcgtg gtcaaccggc ttgagggcag cgaccatcct cgccctctgc tcttcaggca
    10561 gcactggctt aggcttatgg cggacgtttc ggtccctggc gacgatcacg tacagttcgt
    10621 ccccgagcct cccggactcc tctaggtagt acaggtgccc ggggtggaga atatcgaagg
    10681 tgccggttgc caccacgcgc ctcacccgac cacctccagc gcaacaggtt ccccgtttgc
    10741 acggtagcac cgccagtcct cccggctgta cgggaatccc acgatgaggt ggtagcgccc
    10801 gaccctcggg aaaaaggaga gatctgcgtt tgaggggaag agcacgccgt tgggatggct
    10861 gtgggcgctg cccgcaacat gggtatcgag aggcatcatg tcatagaaaa ggctggcgct
    10921 ctcctcgctg ctcacggtcc ctggtaacag gaagagctcc tgcagaatgc cctcctcctc
    10981 cataatcacc cctgcaaact cgtaaggatg acgatccttc cccacttcca ggaggaggtc
    11041 caggaggtcc gtgctgatgc cccgtatctg catatgcttc atcataattg gcaagaagac
    11101 ggcattaaac catggtgcag ggagaaaggg tgacatcccc gggtcctgcc tgcaacaccc
    11161 ccgttgtccc ctgtcagaag ggcccgtcct gctcgaaact ccggccgaac cggatcgcca
    11221 gctctgcctt gtagagttcc tttcccaggt acgcggcatg gtcaaggagt gagacctcct
    11281 ctcctgcaag gatggcgaaa aagacttctg cccaggatgt tccccggtat gcccggtctc
    11341 ttatgactgc gacaacctct gccccctcta tcccgatgcg aaagttccca tgcgggtcgt
    11401 actcgatctc tgcaggcacc gggcctgcct gcacaaaggt ccggtattcg agcgggggct
    11461 ctctccgcct ccgcttctcc ttgatcacca gcaggtcgag gccgagatcc ttcggatagg
    11521 gccggtccct ggagaggacc atcatctcgg tcgccctccg catctcccgg actgccccct
    11581 gcgtcttgtc gctatgctcg ctcgtgaaga tcaccgaggc accgatttct gcggccatcg
    11641 cgcacaggag cgcgtttgcg ccgacagaat cggcatccag gagctcgacg acgttgcccg
    11701 ccccaaagaa gagggggacg tcccatcccc tgaaccgggc cagggagtcg accaggcctg
    11761 atcctgccgg ggggaggagt gggtctgcga caaggcactc gactcccgcg gcccgtgcca
    11821 ttgccaggtt ctcccgaagg ccggccctcc cgggtactac cacggccact gctcccgccc
    11881 cggcaacaag agaccccaca agggggatgt tctcctcctg cagggagagc accatgtcgg
    11941 ccctcggcaa cgctgcggca atcagcgccg ggtcctgcgt gtccacggaa agtggcccgt
    12001 cgatatcttc cagggcagag aaggcagagc ggacctggtc aaatgtggcg tcgaacccga
    12061 acccgaggtc aacaatatcg gctcccagag agaagagagc ctccaccctc gcgcggaggt
    12121 ccggtacccg gtgtgcgtcc atgatctcgg caaggacctt catgcgggaa cctcccccga
    12181 tcttcactcc cctgacggag tagaggaagt ccgactcctg ctctgcagat gcgatgcgca
    12241 actccgcctt cttcctcctc tctccggcca aaaactcgtc cgctggtatc gtcctcgaaa
    12301 gccttacccc cgggagaaga gggaggacga gatcgagatc tgcggcatgc ctcgggccga
    12361 gatagaccgg gactcccgat tcggcctcga ccgatgaaaa cgacgccgtg cacatcccgg
    12421 agacgattgc catgtcatac ttctcctcgg agaggagctc ggcaagcctg gccggcgaga
    12481 gaaacgaggc gatctctccc gtgaccacca cgcgggcatc gattccggca gcggcctctc
    12541 tgaccacctc cagggtcgct ctcccggtgg ggaggaggat tcgcataagt cttcttaata
    12601 tcacggagga caaaaaaggt atgatcagat gctgaactgc gatctgcaca ttcacacaag
    12661 tttttcccgc gacggggaga gcagcgtgga agagatcgtc aggagagcag agctcgtggg
    12721 gctcgatgcg atcgccatca ccgaccacga cacggtggaa ggcgccctcc atgccctgac
    12781 tgttccgacg aggctcgtca tcatcccggg aatcgaagtc tccacccggc aggggcacct
    12841 cctggtgctc ggggtcacga cccccatccc ccgcggaatg gaggtgaccg agacgatcag
    12901 gagggcccgg cagcttggcg gcctcgtgat cctgccgcac ccttaccata tatggcgcca
    12961 cggcgtcgca aggaggatgc ggtcgcccct ctcagaggtg gacgcgatcg aggtcttcaa
    13021 cagcaggtac atcgtaggtt cggcaaaccg caaggccgca cggatcgccc gccggctgaa
    13081 aaaaccctgt gtcggcggtt ccgatgccca caacgcgaga ttcgtgggat acgggaggac
    13141 gctcgtggac gcagcaccgg acactgcgtc gatcctggat gcgatccggg cgggaaggac
    13201 ggtcgccacg gggaggatga ccccccttcg cacctacacc cgccagtccc tgagcaatac
    13261 atggaagaag ttcacacggg gaatgcagcc caggtaaggc tggccttccg ctgcgggtac
    13321 ctgggagaag ggttctttgg atcccaggtg cagcctgtgc tccggacagt ggagggcgag
    13381 ttcatccggg cgtgcctgaa cctcggcctc ttctccagcc accgcggggc gcggttcctt
    13441 gcagccggga ggaccgaccg cggtgtccac gcgaggggcc aggtcttcgc tttctcgacc
    13501 gttcatcccg accgggccgt ctccgcattg aaccggcggc tcccaccgga tttctggacc
    13561 accgggtatg cagtggtgga acccgggttt cacccccggc accatgccgt cagccgcacg
    13621 taccgctatt atttcgggga acctgacctc gacgccgaag cgatggaccg tgcagcgcgg
    13681 atcttttgcg gggtccatga cttctcgctc tttgccaggg ttgaaggaag ggacccggtg
    13741 aggaaggtcc tgacctccag ggtattcctg gaccaggggc tctgcgtgta tgaggtctct
    13801 gccgagagct tcctgtggca catggtcagg tacatggcct ctacgctcct catggcaggg
    13861 ctgggggctg atggcgatga ggtcatcctg tccagacttg cgggggatgg gcgtgccagg
    13921 ctttcccctg ccccccccga aggcctggtg ctctgggatg tggcgtatgc gttcccgttt
    13981 gtccccgttt ccgcgggcga aaaagcgctg gcgctgctgg agagccgggg tgcatatcac
    14041 aggtgcatgg ggcgggtgat tgatacgctg cgggatacca cgaacgacat gtgacgagat
    14101 gccagagttc ccccggcctg atgcagaaga tgctgcatca gccttggacg ccacatggtt
    14161 atcaagagaa gcgtatctgc tgttctccca cattctgcgc gttggggtga ctgaggagag
    14221 cctgcccgtg caggtcagcc ctggacttcg cgacctcttt ctctcacgtt ctcctccctt
    14281 cttcgaaaaa gggtggggcc ttttcctcct gagggcggcc gacgggtctt tttcctgcag
    14341 ggtccactcc tcccgtccat tgatatgctg gatgtacagg tgctgcacca ttgcgcattc
    14401 tcaacagcaa tgggtctgaa gccgggaaac gtgaaggaag atgctccctc tctaccagtg
    14461 acccgttact cacaggggtc ttggaggagg tgttgatgcc ctcctccact ctgcctgaag
    14521 accagtttgc ccggcactgc agttcggtcc tcgccacgca cgggtattcc tgcgagatct
    14581 acgacgactg atcccccttt ccgccggatt tgccctcaac aacacattca tgtattttta
    14641 ctcatatgag taattaaggg cacagggccc acaggcaggt tatgaaaatg ccaggatttg
    14701 atggaacagg accctccgga cgcggtccta tgacaggatg gggaagaggc cgatgcatgg
    14761 catacggggt ccccgcggcc gcacagggcc aggcacaggt tgctgcaccg tttccaccgg
    14821 gttacgcacc ccccgcatat tatggtttgg gcaggggtgg catcccccgg ggttgtggaa
    14881 gagggtttgg cggcggaagg cgaaggcgct gcttctggtg atgaggcatc accggatatc
    14941 cacaagtgag caatcctgca gggtcggcgc tatgactgaa cacacgaagg acaaccaggg
    15001 agtctccctt ggactccctt cttcatctca aacacctcgg acgcacggga caaaatccca
    15061 ggtcacctcc atcccgctgc ggaccgcgct tcgcatcgcg cgcgagcagc agagatcgaa
    15121 gtcctcgcga aggaagatat tccgtgaaga gcctgagcgg tacctggaca tcgtgcacct
    15181 gatgaaagac ccaggctccg aataggtatt tcatgccgcg ggaatccccc aatctttctc
    15241 ttatcttttc ttgactgcag tcccgcaaac gcagaaggct agatgtgccc cgggggagac
    15301 aggtacaggc atggacgaat acattcccgc tgcatgcgca cccaggagga gaggaaggcc
    15361 caggacgcag cgcgtgatcg gaaacatgtt ctcttcccgt tgttattccc cccagtgctg
    15421 gccccacgag gagcgggcag tagtcacgat ccttcccgag gaggtggagc tccttcgcct
    15481 tgttgacctc gaggggatgg agcaggagga ggctgcagcg gccctcggtg tctcgcgaag
    15541 gaccgcgtgg cgggaccttc atgaggccag gagaaaagtg gcggatgcac tcacgacagg
    15601 aaagagtctc cagatcggcg gctgctcccg ggcatccagg gggatatgcc cgaggaaatg
    15661 cccccggttc cctggcaact ctccttgaga gggtttcctc gccgaccgtc cccgccaccc
    15721 ccgttatgca cgtgtctcca catccctgac cggatcctcc cctgcagagg gccggaggtg
    15781 acaattacat catctttatt tctttctact catatgagaa ataatagatg acgccacgcg
    15841 gagagaccgg gggtcaagtg atctctgctg cctcccgggg tcagctcctg cagttggaaa
    15901 ccatgcaagt gtgaggatga tatgaaacac ccctttattt cagtccagaa cctctcaatg
    15961 tcattttcag gaaaaaaagt ccttgataac ctttcgttcg atattcctga aggtgagata
    16021 atcgggatca tcgggaggag cggtgccgga aagagcgtgc tgatgcacct gctgcgcggt
    16081 gtggatgagc cccccacgga ggggtcgatc gtctaccacc tctccaggtg tccgaaatgc
    16141 tcctacatcg gcctccggcg cgaactctca aaaccctgcc cgcgctgcgg tgagcagctc
    16201 tttgcaatcg acgtggactt ctgggagcct gagaacgagc agttaaaaag cctcgttatg
    16261 cggcgcacgg ccatcatgtt ccagcggaca ttcgcgctct atggtgacga cagggtcatc
    16321 gagaacgtgc tccgggcact cgatgatgtc gggtactccc aggacggtgc ggtcaaccgc
    16381 gcggccgacc tcctcgacca ggtacgcctc tcccaccgca tgatgcacat cgcacgagac
    16441 ctctcagggg gcgagaagca acgggtggtg ctggcaagac aactcgcaaa ggagccctgc
    16501 ctcctctttg cagacgagcc gaccgggacc cttgaccccg acaccgcccg gctcgtgcac
    16561 cagatgctcc ggtctgctgc agagaccacg aacatgggga tcgtcgtcac ctcccacttc
    16621 tcccgggtca tccaggacat cgccaccagg gccatgctcc tcgaggaggg gaggatcgag
    16681 aagatcgggg cccccgcaga ggtgataaac cacttcctgc gggacttcca cgaggaggag
    16741 aacatatcgg aggtcgaact gggcgaggag atcctggtgg cgcgtgatgt ggccaagcgc
    16801 tacatgtccc ctgatcgggg gttgatcagg gcagtgaatg gggtcacgtt caaggtgaac
    16861 cgaggtgaga ttttcgggct tatcggcaga agcggcgcgg gaaagacaac gctctcccgc
    16921 atcatcgcgg ggatcatcga gcccacggcc ggggagatga acttccttgt cggtgacgac
    16981 tgggtcgaca tgacaaaacc gggtatcgac cagcgggggc gtgccaagag ctacatcggc
    17041 ctccttcacc aggagtatga cctctatcct caccggacgg tgctcgacaa cctgaccgat
    17101 gcgatcgggc tcgagttccc aaaggagctt gccatgagga aggcacatat caccctctca
    17161 atggcagggt tcccccagag caagagcgca accattctct cccagtaccc cgaccagctc
    17221 tctgagggtg aacgccacag ggtcgcgctc gcgcaggtgc tgatacgcga gccgagcctc
    17281 gtgatactcg acgagccgac cgggacgatg gaccctctca caaagcggga cgtgaagcat
    17341 tcgatcctcc atgcccggca ggagatggac cagaccttta tcctcgtctc ccatgacatg
    17401 gaattcgtgc aggaagtgtg tgaccgggtg gcctggatga agggaggaaa ggtggtggca
    17461 ctcgggagca cggaggaggt ccttgcggag atgccggccg aggacagggg catgcagggc
    17521 cttgcgggac agcccagccc ggcactctga acccttcgag aagggttctt tggcctgata
    17581 cgcccacttt tttt
//
LOCUS       DAOQYR010000045        17490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_3633_length_17490_cov_8.572985, whole genome shotgun sequence.
ACCESSION   DAOQYR010000045 DAOQYR010000000
VERSION     DAOQYR010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 17490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17490
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3633_length_17490_cov_8.572985"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="PKM87_06730"
     CDS             <1..1214
                     /locus_tag="PKM87_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNQ29799.1"
                     /translation="LDEAAQRLAEHLGRQEFVEVYAHHDADGIAAGSILCMALLRRGI
                     HFRLRVLARVSPAAISPETPTLLCDFGSGLEDLPEDVMVVDHHIPYFAGELQVNPRQH
                     GIDGDRDLSSAGAAFLVARELGDNRDLAGLVMLGILGDGQALAGTNLSIFNDAIAQGI
                     ITPGQGFLLPGRDDHERLFSALNPYLHQVSGDEMAVSDLLESASSEDGISLEKLLSLL
                     VLRVSPFAPAETLEGIYGARYSLEREVIPDAHALTALVDACGKSGYGGLASSLCLRSS
                     GNLEEAWNLSFRHRDMVVHALRAACTNEGAPGFVEVEDAQVASDVADALSRDCTQQGP
                     VLVAARRDELCHLSARCPRGMEMELGGAVREAAIQCGGFGGGHRLRAGATIPCARLEQ
                     FRTILEKAVGA"
     gene            1211..1498
                     /locus_tag="PKM87_06735"
     CDS             1211..1498
                     /locus_tag="PKM87_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HNQ29800.1"
                     /translation="MIEIRGGIRTRHRHAGCVAPSLLPDNLQSMHTREEGDLVVTTIE
                     GTSLRSVIASVDDYLMNLAVADEMCALEGGQVRPGTNGRDMDQGTRESPGE"
     gene            1528..2121
                     /locus_tag="PKM87_06740"
     CDS             1528..2121
                     /locus_tag="PKM87_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3ae"
                     /protein_id="HNQ29801.1"
                     /translation="MAKKKQAGRRVEGWKAKSWYKVYTPEVLGKTYIGDTIANDAEGV
                     VGRIMQTTLGEIINDYARQNVKMKFRISNVAGDSAYTEFLGHELTRDYMRSMIKRRTS
                     RIDCHVPVMTKDGKKLDLTVTCFTLTRANLSQVHAIRNLISTMVGKIGAESEMNAIIN
                     GIVNGEISREVFKAVKPLFPVRRVEVIKSKVGVAKVV"
     gene            2239..3474
                     /locus_tag="PKM87_06745"
     CDS             2239..3474
                     /locus_tag="PKM87_06745"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNQ29802.1"
                     /translation="MTAHKILLLVLDGISDRPCPELGNRTPLQAALTPVLDRMARDGI
                     CGIMDTIGPGIRPGSDTSHLSLLGYDPYKYYTGRGPLEAEGSGIHMEPGMIGFRCNYA
                     TLDDGGRVTDRRAGRIHGTEPLSAAIQDGADLSSLGVEFLFRSGTGHRAALAFRGEDL
                     GVSVTSNDPKKEGVPPLPFSARDGTDADRKTARALQEFVRQADGILKDHPLNASRASQ
                     GLPSASTILIRGAGRMGHFEPFSSRYGLKGCVISAAALITGIGTAVGLTRIEVPEITG
                     SADTNLAGKVRAAIDAFRTMDFVLMNIKGADEAGHDGKARAKMEFIEKIDSALAPLIS
                     LEDCIVAICADHSTPCSVKDHSADPVPLVICGEGVRVDRVTHFDEVSCAEGGLHRITG
                     ASLMPILLDLLNRSPKYGA"
     gene            3481..4344
                     /locus_tag="PKM87_06750"
     CDS             3481..4344
                     /locus_tag="PKM87_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29803.1"
                     /translation="MAADIPTHETLVSKVLELPGVRFIEPAFADLPEDTFTAWMEDLP
                     ADQGRLLVDEDSDPLAMAFLTGQGWLAGSLHLREPTLALIEHFEEMNGDIFQEDRAVW
                     EEAVREYYSLLLIETTTPALEDLNPARTAQVRDLLAESWGDRAGISCLDCGCGSGLGS
                     FLLRERGMRPLSYDNDASLLSLGLSRGRLLPGETMCIDATYASRYCGGAEAGLGLMFG
                     EINAFNEQDWEMIAAELLALTNDCLVTVGTEREARLIQSWAEARRCRVEVRENDRDPI
                     YDRWVCLIERA"
     gene            4346..4834
                     /locus_tag="PKM87_06755"
     CDS             4346..4834
                     /locus_tag="PKM87_06755"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNQ29804.1"
                     /translation="MADLPSPTSRNLVRLPGGELLYTPSPRLRAFYLLLLILVVWIGI
                     LPWFILAAVTLPGHLTLTLVLPLLLVILAIRWYIPKYWASIRFRFNATGVVCEKGVWR
                     VDRRVVPYSKIRGVEIACGPFCRYQGIASLRIVLDDGSTLRLPGVEDPAEVRRIIKAE
                     IM"
     gene            complement(4885..5661)
                     /locus_tag="PKM87_06760"
     CDS             complement(4885..5661)
                     /locus_tag="PKM87_06760"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HNQ29805.1"
                     /translation="MMQVMLEAKERKNPNVPIEAEEITPAHFIENRQDLSVWRGNRKV
                     PLEEVFEVTINGEASSAEDVEVIFTGESGKIKRVGEYMESGTIRIEGDIGMHCGNFMS
                     GGTIEIRGNADAWLGREMRGGTITCHGNAGDYCGSGYRGEKRGMRGGTIEVLGNAGDF
                     TAETLSGGTIIVHGNVGDLSGVEMKGGMFTIHGNASRVCGNMTGGTCMVYGTVTDMLP
                     TFRKVGTREGNNGGIALTEFTGDVANRGKGRLFVHSYSFA"
     gene            complement(5826..7589)
                     /locus_tag="PKM87_06765"
     CDS             complement(5826..7589)
                     /locus_tag="PKM87_06765"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HNQ29806.1"
                     /translation="MNSGRTYKQGEQLYYKESPEYSEQTSLCFINPIDLFTLGIEEGE
                     NIEIKTSTGNTVFRTVACYDLVPGEIFLPCGPYANFILPPNTHSTGAPDFKTLEVEVR
                     PTERERVSAWDLLEYEGGTRYDAPPEGCPTISLEGDKTVTDVLCPLCGCVCDDIELGI
                     RDHRIVSCQNGCLLCNAKFLAKNRLITPIKKTVGGWEKVSYEEAIEYIADVLVAAERP
                     LLFGWSGTHGEAQCIGVSIAELIGGVIDNCSSECHGPSIMAIQEVGHPGCTLGQVRNR
                     ADVVIYWGSNPIASHPRHMSRYSTYADGFFLDNSFRNRTVIVFDVRKTETAKVADEFV
                     RVRSGGDYAVFSALRAIIQGKEDVLPKSVAGVAKEELIRISRIMLGAKFGTFFTGIGL
                     TQSRGKYKNVRNAIELVDELNRHTKYTLTPMRGHWNVYGTNQTFTYMTGYPYAVDFSH
                     GVAYYNPGETSAIDMLSREEVDACIIIGSDPGAHFPRACNEHFSRIPTIVIDPFPIMS
                     TAVATMHIPVAMTGVDAEGTAYRMDAVPLWVQKVMEPTQPDDARLLSRIYDAVRKRKG
                     MPQIKGEDAGVFGSPVFSTEK"
     gene            complement(7872..8081)
                     /locus_tag="PKM87_06770"
     CDS             complement(7872..8081)
                     /locus_tag="PKM87_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29807.1"
                     /translation="MGLTRLPMGITLDRLHRNIEMGLQKSTSEPDACWIPIDPVGPIR
                     IPMSVAYIDGYYYDTPWIPHVYIPG"
     gene            complement(8407..8880)
                     /locus_tag="PKM87_06775"
     CDS             complement(8407..8880)
                     /locus_tag="PKM87_06775"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ29808.1"
                     /translation="MLRPVLPALIAMLLVSACGCLAFTAQPAGSRGTDPVLPAPGVSD
                     TKMGARVIAMAVCADEIGTPYPEAADFLIKGLHCNANGGRFSEAIDYYDRALAIDPGC
                     STAWLAKGVALHNLGRYDEAILCYERASALDPGNEGIALLKEMAEGDRVEEHDTV"
     gene            complement(8885..10927)
                     /locus_tag="PKM87_06780"
     CDS             complement(8885..10927)
                     /locus_tag="PKM87_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNQ29809.1"
                     /translation="MNVIVQPQKGNYKILFLDNLQVRAVGIAEIDATPKGYRPGHYRV
                     RWGGKHQFRSTPSKELVVSLRKASVYLTQADEAFEGFLHDFQIPFTRTSVCRICLMED
                     RVTPLNRKNGIRYTTGEQICFDCARRELRRELATMGKVGMQALGHLEELLRNYRDMDK
                     VLASVQPEKVKISQALFDRLDAHPVQKTQSLDELPIPRAFIDVAGVETLMPAQQMAVD
                     GGLLYGKHLLVVSATASGKTFIGEMAGMKNYLEGRGHTLFLVPLVALAVQKYQRFEER
                     YGKIAGVGVLIGQARIRLPENAPVGDRNIRAPIVVGTYEGVDHFIRSGKRLPPIGTVV
                     IDEVQMLEDPDRGHRLDGLVARLKYLAPKAQFLYLSATIGVPKVLAKKLDATLVRYDD
                     RPVALERYLIFVEHKQKISTIKRLCTQEYSRVSSKGFHGQTIVFTHARARTHVIADAL
                     GPGYAAYHAGLTSQERREIEAKFLSGKIKAVVTTAALGAGVDFPASQVIFDSLAMGIS
                     WLSVQEFNQMAGRAGRPDFHDLGKVVVLAEPGASYSRSSGSTEEEVAMKLLKGEMEEV
                     APEHDLEQSSEEYVANAVVCQGRLSELDRINGTMVGTMEPVLDILLGKDLVTRQEERV
                     VLTPLAKVMAEHFIGVERLGEIRRLMRKIDDPLEIVAELECAEMEQKKEKEKKRRK"
     gene            complement(10924..11733)
                     /locus_tag="PKM87_06785"
     CDS             complement(10924..11733)
                     /locus_tag="PKM87_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNQ29810.1"
                     /translation="MTEASKDPLSSILRSKREVTRLQILVEIAEHQPAVRQQEIADKL
                     GVTPQAVSEYIRELVDEEMAAAHGRGRYSVTRTGIEWILNHAEALEAYARHIRRDIIQ
                     QVSTWTAIAAEDLFEGDQVGVYMEHGWLYASRESRSAMGTVVMDAKAGEDVGVARLSG
                     LIDHTEGVVHVCKVPRVQRGGSRKVRKDRLLEVIEGVGVVAAVGLEGYVALQQAGRTP
                     DMFFGAREGVIEAAFHGIDCAILVVDEEFTDFLKRLEGVGLSYILHDLIAS"
     gene            complement(11717..12229)
                     /locus_tag="PKM87_06790"
     CDS             complement(11717..12229)
                     /locus_tag="PKM87_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNQ29811.1"
                     /translation="MSGHIRIVNDPVDLVPLLITFHNATYKQVYDLLNKSWMTEEEIC
                     EQLAEGDVGQCLLVLKKGSLVEEKWRMPAPGQKPVKEYRASYNKFRANFQCNFGDLSD
                     LLFIAVSNDEHLRCMVEKVEDELRSGNTSINDIARKFGVNPVFIKGLAKRTPHLDVKG
                     QGLVLLDRSQ"
     gene            12278..14521
                     /gene="hypF"
                     /locus_tag="PKM87_06795"
     CDS             12278..14521
                     /gene="hypF"
                     /locus_tag="PKM87_06795"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HNQ29812.1"
                     /translation="MNGKRRSGRIQIRGIVQGVGFRPFVYATATRLGIRGFVRNRGSE
                     VEIQAEGDQFEAFLEEVSRGPPLSRIDRVEVLQDPGPFPGDFSIQPSGSGSLSGMIPP
                     DIATCDQCLQDIHTAGGRYSGYWCTSCVNCGPRYSIIRDLPYDRERTSMDAFPMCPEC
                     KDEYHDPASRRHHAQTIACASCGPKLRLFDGEGNDVSSGDIIAETARILDAGYIVAIR
                     GIGGFHLACIESSAGELKRRLGRSEQPFAVMVRPDVVDRIAILSAEDQMLLESCQRPI
                     VVLGKRDPDAHQDISNLHTIGCMLPYTGLHHLLFSRLSHPLLIMTSANMPGYPMITGI
                     DVAMARMHGHVDFFLTHDRQIVNRCDDSVVRDGFIIRLSRGMAPRRTAIDLGNTSILA
                     VGPELNANVTIYRDGFCTTSPHVGNVRNPQTYAYLQETIENLGRLLGRDYKYIAHDAH
                     PQFLSTRYARELAGEHGAALVPVQHHQAHIAATTTSPCIGIAIDGVGYGEDGTIWGGE
                     IFAGSIPGYERVGHLEVVPMPGGDLATRFPERMLYGILPTPSIAALLSSRGWTDLELS
                     VLDSQISRGINVVPTSSTGRVLDAAAALLGICRERTYDGEPAMKLESFAVGGKARQWD
                     IDYIQVGGKEVFSTRALLRKAMGEMQRCKDHDQRAMRSIAASFQFNLARGIAGMAIHA
                     AYKRDLPIVALSGGVAYNRVIRETIRNLVTTSGLELRMNPDYPLGDGCISYGQCVYAG
                     VSAGIEK"
     gene            complement(14565..15938)
                     /locus_tag="PKM87_06800"
     CDS             complement(14565..15938)
                     /locus_tag="PKM87_06800"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNQ29813.1"
                     /translation="MKMTYHSTMKLIALILLIGALFSVVQATTITAEGGAIPTVGETR
                     DFPIVADEFPDGLLGYSLNATLTDPSKAEIFDVLFPEWAPSYPFSYYTTTPADEVGIR
                     IFGYQVNSGQTNVTLAILRIRGDAVGQTDVNITVKTLQDGSGAKITATTVPGTIIIGT
                     PPTTTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTT
                     ATTTATTTATTTVTTSPTATATTTATTTATTTATTSPTTTTTTTPPADPGYLTVFSFP
                     LKATASIDGTVVGITPLMGYRVSPGIHTLKVSMPGYLDYTTSVSVTSGGHLQVPLIVL
                     QRGIPTPTQTTQTTPATPTPSVSPTATITTAITTPPTTTTTTSTVTTSPVQTGSLTVN
                     TVPRGVTIYLNNEARGTSPTTIHGLAPGDYELKLMKQGFKTQVRSVTIETGKTTTPPL
                     IILTPIL"
     gene            complement(15971..>17490)
                     /locus_tag="PKM87_06805"
     CDS             complement(15971..>17490)
                     /locus_tag="PKM87_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ29814.1"
                     /translation="GTNSTSKPFYIDVGVPLIVNFSANVSDVECAFCQGQVPFDVNFT
                     DLTNDTPLSWNYSFGDGNFSINQHPNWTYHIPGLYNVSLNVTNEYGFNSTTKYFLVEA
                     GDVPIANFTNATPRFGVVPLNVVFTDLSFGIPNITAWNWSFDNTTAVEWFNTTVPAQR
                     NANHTFLAKGNYTVNLTVFNLYGNDTMSKTDFVRVGELPVANFTADNTNVIEGDLVNF
                     TDWSTGYPEWWQWNFGDGTILDRTENTTMMPVNHTYDNSGNYTVALTVGNEFGTNTLP
                     RSRYIQVSGNVFYTNLYFVPSSFLIPTNSTTQVRLEAEKFDQGLGIYNITVYFANPAA
                     AQFVEMNPPSWADPTWTRNSTVPGSSVWLRVADVPDAIKPGATNVTLATFNATGYVPG
                     STTINVTVELMQADTGDNIQTQSTPADVEIVLLQQFPPTENWPIYGPPTAPYHDGVYW
                     DLNGSGDIDFVDVVLFFLLFDEWMSEPGQPIALFNYDGIDGLDFNDLVLLFNEVP"
BASE COUNT         3421 a         5237 c         5111 g         3721 t
ORIGIN      
        1 cccttgacga ggccgcacag aggctcgccg aacacctcgg caggcaggag ttcgtagagg
       61 tctacgccca ccacgatgcg gacggcatcg cggcaggctc catcctttgc atggctctcc
      121 tgcgcagagg catccacttc cggctccggg tcctcgcccg ggtgagcccg gcggccatat
      181 ccccggagac ccccacgctc ctctgcgact ttgggtcggg gctcgaggac ctcccggagg
      241 acgtgatggt ggtcgaccac cacatcccct actttgcagg agaactccag gtcaaccccc
      301 gccagcacgg gatagacggc gaccgcgatc tctcatcggc gggggccgcc ttcctggtcg
      361 cacgggagct aggagacaac cgggacctcg ccggcctcgt catgctcggc atccttggag
      421 acgggcaggc cctggcgggc accaacctct ccatcttcaa cgacgcgatc gcacagggaa
      481 tcatcactcc cggacagggg ttcctgctcc cgggaaggga cgaccacgag cggctctttt
      541 cggccctcaa cccctacctg caccaggtga gcggggacga gatggcggta tcagacctcc
      601 tcgagagcgc ctcttctgaa gacggcatct cgctcgagaa gctcctctcc ctcctcgtgc
      661 tgcgggtcag cccgttcgca ccggcggaga cgctggaggg gatctacggg gcgcgctaca
      721 gcctcgagag ggaggtgatc cccgatgcgc acgcgctgac cgcactggta gatgcatgcg
      781 ggaagtcggg gtacggcgga cttgcgtcct cgctctgcct ccgctcctcg ggcaacctcg
      841 aagaagcgtg gaatctctcg ttccggcacc gggacatggt ggtccacgcg ctccgtgctg
      901 cctgcacgaa tgaaggggcg ccgggctttg tcgaggtaga ggacgcccag gtcgcaagcg
      961 acgtcgccga tgccctctcc cgggactgca cacagcaggg accggtgctg gtggccgccc
     1021 gccgcgacga gctctgccac ctgtccgccc gttgcccgag gggcatggag atggaactcg
     1081 gcggggccgt ccgcgaggct gccatccagt gcggcgggtt cggggggggc caccggctca
     1141 gggccggcgc caccatcccg tgcgcacgcc tggagcagtt ccgtacaatc ctcgagaagg
     1201 cggtgggcgc atgattgaga tccggggcgg gatccggaca cggcacaggc acgcagggtg
     1261 cgtggcgccg tctctcttgc ccgacaacct ccagagcatg cacacccgcg aggaaggaga
     1321 cctggtcgtc accacgatcg agggaacctc gctccggtcg gtgatcgcgt ctgtggacga
     1381 ctacctgatg aacctggcag tggccgatga gatgtgcgcc cttgagggag gacaggtgcg
     1441 acccggaacg aacggaaggg atatggacca ggggactcgc gagagccccg gagaataaca
     1501 gaacaatcct atcagaatgg tgaatacatg gcaaagaaga aacaggctgg acggagagta
     1561 gaaggctgga aagccaagag ctggtacaag gtgtacaccc ccgaggtgct cggaaagacc
     1621 tacatcgggg acaccatcgc aaacgacgcc gagggcgtgg tgggcaggat catgcagacc
     1681 accctcggcg agattatcaa cgactatgca cggcagaacg tcaagatgaa gttccggatc
     1741 tcaaatgtcg caggggactc ggcctacacc gagttccttg gccacgagct gacccgtgac
     1801 tacatgcgtt ccatgatcaa gcggcgcacg tcgcggatcg actgccacgt cccggtcatg
     1861 acaaaggacg ggaagaagct tgaccttact gtgacgtgct tcaccctcac ccgggcgaac
     1921 ctctcccagg tgcacgccat ccgaaacctc atctccacca tggttggaaa gatcggtgcg
     1981 gagtccgaga tgaacgccat catcaacggc atcgtgaacg gcgagatctc gcgcgaggtc
     2041 ttcaaggcgg taaagcccct cttcccggtc cgccgggtcg aggtcatcaa gtcgaaagtc
     2101 ggcgtggcca aggttgtctg aaaggggcac cgggcccctt cgtatccagg cccaaccgcc
     2161 ttttttatct cttttcgcct cgctgcagcg cgggggatgg gtcaagcata agacagggaa
     2221 cagactactc ttctggtgat gacggcgcac aagatcctcc tccttgtcct ggacggcatt
     2281 tcggaccgcc cgtgcccgga gcttgggaac aggacacccc tccaggcggc actcactccg
     2341 gtgctcgacc ggatggcccg cgacgggata tgcggcatca tggacaccat cgggccgggg
     2401 atacgccccg ggtcggacac gtcgcacctg agcctgctcg gctacgaccc ctacaaatac
     2461 tataccggcc gggggccgct cgaggcagag gggagcggga tccacatgga acccgggatg
     2521 atcgggttcc gctgcaacta cgccacgctc gatgacgggg gacgggtcac tgaccgccgg
     2581 gctgggagga tccacggcac cgaacccctc tccgcggcga tccaggacgg ggcggacctg
     2641 tcatcgctcg gcgtcgaatt cctgttccgc tccggcaccg ggcatcgcgc cgccctggcc
     2701 ttccgcggtg aggacctcgg ggtctcggtc acgtcgaacg acccgaagaa ggaaggggtg
     2761 cctccgctcc cgttctcggc ccgggacggc accgatgcgg acaggaagac cgcacgggca
     2821 ctccaggaat tcgtgcgcca ggccgatggg atactcaaag atcaccccct caacgcgtcg
     2881 cgggcatctc aaggcctccc ctccgcgagt accattctta tccggggagc gggaaggatg
     2941 gggcactttg agcccttctc ctcccggtac gggctcaaag ggtgcgtcat ctccgctgcc
     3001 gccctgatca ctgggatagg gacggccgtc gggctcaccc gcatcgaggt cccggagatc
     3061 accggctcgg ccgatacaaa ccttgccggg aaggtccgtg cagccatcga cgcgttcagg
     3121 accatggact tcgtgctgat gaacatcaag ggtgccgacg aggccggcca cgacgggaag
     3181 gcgcgggcga agatggagtt catcgagaaa atcgattcgg ccctggcgcc gcttatctcg
     3241 ctcgaggact gcatcgttgc catctgcgca gaccattcca cgccgtgcag cgtgaaagac
     3301 cacagcgccg atccggtgcc gctcgtcatc tgcggcgagg gtgttcgggt cgaccgggta
     3361 acgcacttcg acgaggtctc ctgcgccgag ggtggcctcc accggatcac cggcgcctcg
     3421 ctgatgccaa tactgctgga tctcctgaac cggtccccga aatacggagc ctgatgaacc
     3481 atggccgccg atatccccac gcacgaaacg ctcgtctcga aggtcctgga actgcccggt
     3541 gtgcggttca tcgagcccgc gttcgccgac ctgccggagg acaccttcac ggcatggatg
     3601 gaggatctcc ccgccgacca gggccggctc ctcgtcgacg aggactccga cccgcttgca
     3661 atggccttcc tcaccggcca gggctggctt gccgggtccc tccatctccg tgagccgacg
     3721 ctcgcgctga tcgagcactt cgaggagatg aacggcgaca tcttccagga ggaccgcgcc
     3781 gtatgggaag aagccgtccg ggagtactac agcctccttc tgatagagac gaccacgcct
     3841 gcgctcgagg acttaaatcc cgccaggacc gcccaggtgc gcgatctcct cgccgaatcc
     3901 tggggagacc gggcagggat ctcctgcctc gactgcggct gcgggtcggg gctcggctcc
     3961 ttccttctgc gggagcgcgg gatgcgcccg ctctcctacg acaacgacgc ctccctcctc
     4021 tcactcggac tctcccgggg aagactcctc ccgggcgaga ccatgtgcat cgacgcgacc
     4081 tatgcaagcc ggtattgtgg gggcgcagag gcgggtctcg gcctcatgtt cggcgagatc
     4141 aacgccttca acgagcagga ctgggagatg atcgccgccg agctcctggc actcacgaac
     4201 gactgccttg tcacggtcgg caccgagcgc gaggcccggc tgatccagtc ctgggcggag
     4261 gcccggaggt gccgggtgga ggtccgggag aatgaccgcg atcccatcta cgaccgctgg
     4321 gtctgcctga tcgagcgggc atgagatggc ggatctcccc tcccccacat cacggaacct
     4381 cgtgcgcctg ccgggcggtg aacttctcta caccccgtcc ccgcgcctcc gagcattcta
     4441 cctccttctc ctgatccttg tcgtctggat cggcatcctc ccctggttca tcctggctgc
     4501 agtcaccctt cccgggcacc tgacgcttac cctggtgctc ccgctgcttc tcgtcatcct
     4561 cgcgatacgc tggtacatcc cgaagtactg ggcgtccatc aggttccggt tcaatgcgac
     4621 gggagtagtc tgcgagaagg gcgtctggcg ggtggaccgc cgggtggttc cgtattcgaa
     4681 aatcaggggc gtcgagattg catgcggacc gttctgccgc taccagggga tcgccagcct
     4741 gcggattgtg ctcgatgacg gcagcactct ccgccttccg ggagtggagg acccggcaga
     4801 ggtgcgacgg atcatcaagg cggagatcat gtaggttaca tcgttctgcg cccggcacaa
     4861 tcataaagcg gggtcaattc gcgtttatgc aaaggaatag gaatgcacga acagtcgacc
     4921 cttgccgcgg ttcgccacat cgccggtgaa ttcagtgagt gcaattcccc cattattccc
     4981 ttctcttgtg cccactttcc ggaatgtcgg caacatatcg gtgaccgtgc catacaccat
     5041 gcaggttcct ccggtcatat ttccacagac ccgggatgca ttgccgtgaa tcgtgaacat
     5101 ccctcctttc atctcgaccc cggagagatc cccgacgttt ccatggacaa taattgtgcc
     5161 accggagagg gtctctgcag taaaatcacc tgcatttccc aagacttcta tagtccctcc
     5221 cctcatcccc cttttttcgc cgcgatagcc ggaaccgcag tagtccccgg cgtttccatg
     5281 gcaggtgatt gtccctcctc tcatctcgcg gccaagccag gcatcggcat ttccccttat
     5341 ctcgatggtt cctccgctca tgaagttccc gcagtgcatc ccgatatctc cctcgatccg
     5401 gatagtgccc gattccatat attcgccaac ccgtttaatt ttccctgatt ccccggtgaa
     5461 gatcacttct acatcttctg cagaagacgc ctccccgttg atcgttacct caaaaacctc
     5521 ctcaagggga actttccggt ttccccgcca cacggaaaga tcctgccggt tttcgatgaa
     5581 atgtgcaggg gtgatctctt cggcttcgat gggaacattt ggattctttc tctccttcgc
     5641 ttcaagcatt acctgcatca tcacaacgcc tccttttcac caattctgct tccctggttg
     5701 cccgcaggag gaccggatcc tcattattcg tttcgttgtc gctcattcgc atatttaaca
     5761 ccctcatggg aacttgtccc actgaccgga gaatccttcc atatatgtgg tgggaaaaaa
     5821 attggtcact tctctgttga gaaaaccggc gaaccgaaaa cacctgcatc ttctcctttg
     5881 atctgaggca taccttttct ctttctcact gcatcgtaga ttcgactgag tagtcgtgca
     5941 tcatccggtt gcgtcggttc catgaccttc tggacccaca gcggaaccgc atccatccgg
     6001 tatgccgtac cctcggcatc gacaccggtc atggcgacag gtatgtgcat cgtcgcaacg
     6061 gccgtgctca tgatggggaa cgggtcgatc acgatggtcg gaatccttga aaaatgttcg
     6121 ttgcaggccc tagggaagtg cgccccggga tcgctcccga tgatgatgca ggcgtctacc
     6181 tcttcccttg agagcatatc gatggccgat gtctcgcccg ggttgtaata ggcgaccccg
     6241 tgggaaaaat cgacggcgta aggatatccc gtcatgtaag tgaacgtctg gttcgtgcca
     6301 tacacgttcc agtgtccacg catcggggta agcgtgtatt ttgtatggcg gttcagttca
     6361 tccacgagct cgatggcgtt tctcacgttc ttgtactttc ccctggactg ggtcaacccg
     6421 ataccggtaa agaaggtgcc gaactttgcc ccgagcatga tccttgaaat ccggatcagc
     6481 tcctcctttg ccacacccgc cacgctcttg ggaagcacgt cctcctttcc ctggatgatc
     6541 gcacggagcg ccgagaagac tgcataatcc cctcctgatc tgacccgaac gaattcgtcg
     6601 gcgaccttcg cagtctccgt ttttctcaca tcaaagacga taactgtcct gttcctgaaa
     6661 gagttgtcaa ggaagaaccc gtctgcatag gtcgaataac ggctcatatg cctgggatgc
     6721 gacgcgatag gattggatcc ccagtaaatg acgacatccg ccctgttcct gacctgccca
     6781 agggtgcatc ccgggtggcc cacttcctgg atcgccatga tcgaggggcc gtggcactcg
     6841 gacgagcagt tgtcgatgac cccgccgatc agttcggcga tcgagacccc gatgcactgc
     6901 gcttcaccgt gcgtcccgct ccagccgaac agaagcggac gttccgccgc gacgaggacg
     6961 tcggcgatat attcgattgc ctcctcgtaa ctgactttct cccaacctcc cactgttttt
     7021 ttgattggcg tgataaggcg gttttttgcc aggaactttg cgttgcacaa caagcaccca
     7081 ttctggcacg acacgatgcg atggtccctg atcccgagct cgatgtcgtc acagacgcag
     7141 ccgcagagag gacagagtac gtcagtgacc gtcttatcgc cctcgagcga gatggtgggg
     7201 cacccctcgg ggggagcatc atacctcgtc cctccctcgt attcgaggag gtcccaggcc
     7261 gacacccgct cccgctcagt cggacggacc tcaacctcca gggtcttgaa atcgggagcc
     7321 ccggtcgagt gagtatttgg cgggaggatg aagttcgcat acggaccgca ggggaggaat
     7381 atctccccgg gaacaaggtc ataacaggca acagtcctga ataccgtgtt tcccgtcgaa
     7441 gttttgatct cgatgttttc accctcttcg attccgagtg tgaagagatc gatcgggttg
     7501 ataaaacaga gagatgtctg ctcggaatat tccgggcttt ccttgtagta aagttgctcg
     7561 ccctgcttgt aggtccgccc gctgttcatc agcattctca tacctgcatc actcctctca
     7621 tcccaaccct gtccatttca tacgtcagga atgatggtgg tatgttaagt atattagaat
     7681 ttacgatatg agcagaaaca tgaggattct ctcattgaat atcaaataaa aattgatatt
     7741 gtcggtttat tgagcatatt tcttcgtaat aataagtgga ccaggtatat catttttcat
     7801 taacatttat aggggcgatc ccgaaccagg cgggagtgcc ccagaggatt tgcatgatac
     7861 ctgaaattag cctatccggg tatgtagaca tgcggtatcc atggagtgtc gtaataatat
     7921 ccatcgatgt acgcgacgga catgggaatc cgtatcggcc cgacaggatc gatgggaatc
     7981 cagcatgcgt cgggctctga agtactcttc tgaagcccca tctcaatgtt cctgtgcaac
     8041 ctgtcgaggg tgatccccat tggcaaccgt gtcagaccca tgttgaattg cgagacctgt
     8101 agggatgccg gcttaatagt ttcaacttga aacttaccca cggaaggatt ggagatttgg
     8161 gatataatga agggtttttc aaatgaagga aacaagagga gactgcgtga atcttgcagg
     8221 aatgacagtg atgtcattgc tcctgaaacc gcaggagatg ttgtgagaaa tagaatggtt
     8281 accacaaaaa ccggcatctt aagggacatc atatccaaca gtgtgaaatt atacgtaata
     8341 ttacatataa attcgatcct tcgtggagat tatcgccgtt ttttaaaaaa cccactcccg
     8401 ggttcattac accgtatcat gttcttcaac ccggtcccct tctgccatct ccttcaaaag
     8461 cgcaatcccc tcgttccccg ggtcgagcgc agacgccctt tcataacaca ggatggcttc
     8521 gtcgtaccga cccaggttgt ggagcgccac ccccttcgct aaccacgcgg tcgaacatcc
     8581 cgggtcgatg gcgagagccc ggtcgtagta atcgatcgcc tctgagaacc ggcctccgtt
     8641 tgcgttgcag tgcaggcctt tgataaggaa atccgcagcc tcgggataag gagtccctat
     8701 ctcatccgcg cataccgcca ttgcgatgac ccgtgccccc atcttcgtgt ccgatactcc
     8761 aggtgccggg agtaccggat ctgttccccg tgacccagcg ggctgtgcgg tgaacgcaag
     8821 gcaaccgcac gccgatacaa ggagcatggc gatgagtgcg ggcaggacgg ggcgcaacat
     8881 ttcactattt cctcctcttc ttctccttct ccttcttctg ctccatctcc gcgcactcga
     8941 gttccgccac gatctccagc gggtcgtcta tctttctcat cagcctccgg atctcgccga
     9001 ggcgctccac cccgatgaag tgttcggcca tcacctttgc aagcggggtg agcactaccc
     9061 tttcctcctg ccgggtcacc aggtcctttc caagcagtat atcgaggaca ggctccatgg
     9121 tgccgaccat cgtcccgttg atccggtcga gttcggaaag cctcccctgg cagacaaccg
     9181 cgtttgcgac gtactcctcc gagctctgct cgaggtcgtg ctcgggggcg acctcttcca
     9241 tctctccctt gaggagcttc atcgctacct cctcctccgt cgatcctgat gagcgggagt
     9301 aggaggcgcc gggctcggcg agcacgacca cctttccgag gtcgtggaag tcgggccggc
     9361 ccgcacggcc ggccatctgg ttgaactcct ggaccgagag ccaggagatc cccatcgcga
     9421 gggagtcgaa gatcacctgc gaggcgggga aatcgacccc cgcaccgagc gcagcggtgg
     9481 tgaccacggc cttgatcttt cctgatagga attttgcctc gatctcccgc cgctcctggg
     9541 aggtgaggcc cgcatggtag gctgcatatc ccggccccag ggcgtcggcg atgacgtgtg
     9601 tccgagcccg ggcgtgggta aagacgattg tctggccgtg gaagcccttc gaggagaccc
     9661 gcgagtactc ctgggtgcag aggcgcttta tcgtcgagat cttctgcttg tgctcgacga
     9721 aaatgaggta gcgctcgagg gcgacgggac ggtcgtcgta ccggacgagc gttgcatcca
     9781 gtttctttgc gagcaccttt gggaccccga tggttgcgga gaggtacagg aactgggcct
     9841 tcggggccag gtacttcagc ctcgcgacga ggccgtccag gcggtggccc cggtccgggt
     9901 cctcgagcat ctggacctcg tcgatgacaa ccgtcccaat cggcgggagc cgcttccccg
     9961 aacggatgaa atggtcgacc ccctcgtagg tccctacgac gatgggagct cggatgttcc
    10021 ggtccccgac gggagcgttc tcgggcagcc tgatcctggc ctggccgatc aggaccccta
    10081 cccccgcgat ctttccgtac cgctcctcga agcgctggta cttctgcacc gcgagcgcaa
    10141 ccagggggac gaggaagagg gtgtggcccc tcccctccag gtaattcttc atacccgcca
    10201 tctcgccgat gaaggtcttg ccgctcgcag tggccgagac aaccaggaga tgctttccat
    10261 agagaaggcc tccgtcgacc gccatctgct gtgccggcat caacgtctcg acaccggcaa
    10321 catcgatgaa cgcccgcggg atgggaagtt catcaagaga ctgggtcttc tgtaccgggt
    10381 gggcatccag gcggtcgaag agtgcctggg agatcttcac cttctcgggc tgaacgctcg
    10441 cgaggacctt gtccatgtcg cggtagttcc gcagcagctc ctcgaggtgc ccgagagcct
    10501 gcatgcccac cttgcccatc gtggcaagtt cccttctgag ctccctgcgg gcgcagtcaa
    10561 aacagatctg ctcgccggtc gtgtagcgta tgccgttctt cctgttcagg ggggtgacac
    10621 ggtcctccat caggcatatc ctgcagacgg aggtacgggt aaaggggatc tggaagtcgt
    10681 gcaggaaccc ttcgaatgcc tcgtccgcct gggtcaggta caccgatgcc ttccgcaggg
    10741 agaccaccag ctcctttgaa ggggtgctgc ggaactggtg cttgcccccc cacctcacgc
    10801 gatagtggcc cggccggtat cccttcggag tggcgtcgat ctccgcgatc cccaccgccc
    10861 gcacctgcag gttgtcaagg aaaaggatct tgtaatttcc cttctgcggc tggacgatga
    10921 cattcatgag gcgatcaggt catggaggat atacgagagc cccactcctt caagccgttt
    10981 caggaaatcg gtgaactcct cgtctacgac gaggatggca cagtcgatgc catggaatgc
    11041 cgcctcgatc accccttctc ttgccccaaa gaacatatct ggcgtccttc ccgcctgctg
    11101 gagggccacg tagccctcga ggccgactgc cgccaccacg ccgacgccct caatgacttc
    11161 aaggagccga tccttcctca ccttgcgaga cccgcccctc tggacgcggg ggaccttgca
    11221 gacatggacc acaccctcgg tgtggtcgat caggcccgag aggcgggcaa ctcccacgtc
    11281 ctcgcctgct ttcgcatcca tcaccaccgt tcccattgca ctccgggact ccctcgatgc
    11341 atacagccag ccgtgctcca tgtacacccc cacctggtcg ccctcgaaga ggtcctcggc
    11401 ggcaatcgcc gtccatgtgg agacctgctg gatgatatcc cttcgaatgt gccgggcgta
    11461 ggcctccagc gcctcggcat ggttgaggat ccactcgatc ccggtcctcg tgacgctgta
    11521 cctgccgcgg ccatgggcag ccgccatctc ctcgtccacg agctcccgga tgtactcgga
    11581 gacagcctgg ggcgtcaccc cgagcttgtc cgcgatctcc tgctgcctta ctgccggctg
    11641 gtgctcggcg atctcgacca ggatctgcag acgggtcact tcgcgcttgc ttcggagaat
    11701 agacgacagg gggtccttac tggcttcggt caaggagaac cagcccctgt ccctttacgt
    11761 cgagatgggg ggtccgcttc gccagtcctt tgatgaagac ggggttgacc ccgaactttc
    11821 gtgcgatatc gttgatagag gtattccctg aacgaagctc atcctcgacc ttctcgacca
    11881 tgcagcggag gtgttcgtcg tttgagactg cgataaagag gagatctgag agatccccga
    11941 agttgcactg gaaatttgcc ctgaacttgt tgtaggaggc acggtactcc ttcaccggtt
    12001 tctgcccggg ggctggcatc ctccacttct cctcgacaag gctccctttt tttagcacca
    12061 gcaggcattg gcctacgtcc ccctcggcca gctgctcgca gatctcctcc tcggtcatcc
    12121 aggacttgtt caacagatcg tagacctgct tataggtcgc attatgaaac gtgataagca
    12181 gaggaacaag gtccactgga tcgttgacta tcctgatatg gccgctcact ggtaaaaccc
    12241 tagtattata tggcgtgata aatcaataaa tctttgtttg aacggcaagc gcaggagcgg
    12301 aaggattcaa atccgtggaa tcgtgcaggg tgtcggcttc cggccattcg tgtatgccac
    12361 ggcgaccagg ctcgggatcc ggggttttgt caggaaccgg ggcagcgagg tcgagataca
    12421 ggccgagggc gaccagttcg aggcgttcct ggaggaggtc tcgcgtggac ccccactctc
    12481 ccgaatcgac cgggtggagg tactccagga cccagggcca ttcccaggcg atttttcgat
    12541 ccagccaagc gggagcggtt ccctctccgg catgattccg ccggacatcg cgacctgtga
    12601 ccagtgcctc caggatatac acaccgcagg agggcggtat tccgggtact ggtgcacctc
    12661 gtgcgtgaac tgcggcccgc ggtacagcat tatccgcgac ctcccctacg acagggagag
    12721 gacatcgatg gacgcctttc ccatgtgccc ggaatgcaag gatgagtacc atgaccctgc
    12781 cagccggcgg catcatgccc agacgatcgc atgtgcctct tgcggaccga agttacgcct
    12841 ttttgatggt gaaggaaacg atgtctcctc cggagatatc atcgccgaga ccgcccgtat
    12901 cctggacgcc gggtatatcg tggcaatccg gggcattggc gggttccacc ttgcctgtat
    12961 tgagagctca gcaggggaac tgaagcgccg cctgggaagg tccgagcagc cgtttgcggt
    13021 gatggtcagg ccggacgtcg tcgaccggat cgcaatcctc tctgccgaag accagatgct
    13081 tctcgaaagc tgccagcggc ccatcgtcgt cctcgggaag cgtgacccgg atgcccacca
    13141 ggatatcagt aacctccaca ccatcggctg catgctcccc tataccggcc tgcaccacct
    13201 gctcttctca cgcctctccc atcccctcct gatcatgaca agtgcgaaca tgccggggta
    13261 tccgatgatt accggcattg atgtcgcaat ggcacggatg cacggacacg tcgacttctt
    13321 cctcacccat gaccgccaga ttgtcaaccg gtgcgacgat tccgtggtca gggacgggtt
    13381 cattatccgc ctctcgaggg gaatggcccc caggagaaca gccattgacc ttgggaatac
    13441 ctccatactg gcagtggggc cggaactgaa cgcaaacgtg accatctaca gggatgggtt
    13501 ctgcaccaca tcgccccatg tcgggaacgt ccggaaccct cagacctacg cctacctcca
    13561 ggagacgatc gagaaccttg gccggctcct cggcagggat tacaagtaca tcgcccatga
    13621 tgcgcatccc cagttcctct ccacccggta cgcaagagag ctcgcagggg aacatggagc
    13681 cgcgctcgtc cccgtccagc atcaccaggc ccacatcgcg gcgaccacga cctcgccctg
    13741 catcggcatc gcaatcgacg gggtcgggta cggggaggac ggcaccatct ggggagggga
    13801 gatattcgcc ggctccatcc ccggttacga acgggtcggc cacctggaag tggtcccaat
    13861 gcctggcgga gaccttgcaa ccaggtttcc ggagaggatg ctttacggca tccttcccac
    13921 cccttcaatt gcggcgctcc tctcgtcccg tggctggacc gatctcgagt tatctgtcct
    13981 tgactcacag atctccagag ggatcaacgt ggtcccgacg tcgagcacag gcagggtgtt
    14041 ggacgctgcc gccgccctcc tcggcatatg cagggagagg acatacgacg gagagcccgc
    14101 aatgaaactc gagtcatttg ccgttggggg gaaggcccgg caatgggaca tcgattatat
    14161 ccaggtcggg ggcaaggagg tattctccac aagagccctg ctgcggaaag cgatgggcga
    14221 gatgcagagg tgcaaagatc atgaccagag ggcgatgcgt tcgatcgccg catcgttcca
    14281 gttcaacctt gcccgtggga ttgcagggat ggcgatccat gccgcgtaca agagggatct
    14341 tcccattgtt gcactttcag gcggtgtggc ctataacagg gtgattcgcg agaccatcag
    14401 gaacctggtg acgacatcgg gcctcgagct tcgcatgaac ccggattacc cgctcggaga
    14461 tggatgcatc tcgtacgggc agtgcgtgta tgctggggtg tcagccggga ttgaaaaata
    14521 gacagatttt ttttaaaatg ggggttgggg attcttcagg attctcacag gattggggtg
    14581 aggatgatga gaggcggcgt ggtcgtcttt ccggtctcga tggtcacgct cctgacctgg
    14641 gtcttgaacc cctgtttcat gagtttcagt tcataatcgc cgggagcaag cccatggatt
    14701 gttgtcggac tggttcctcg cgcctcgtta ttgaggtata tcgtcacacc acgggggacc
    14761 gtgtttacag taagtgatcc tgtctgcacc ggggaggtgg tgacagtaga ggtggtcgtg
    14821 gtagtggtgg gtggagtcgt aatcgcggtg gtgatcgtgg cggtcgggga gacactcggt
    14881 gtgggtgttg cgggagtggt ctgcgtggtc tgcgtgggag ttggaattcc tctctgcagg
    14941 acgattaacg ggacctgcag gtggcctccc gatgtgacgg agacacttgt ggtataatcc
    15001 agatacccgg gcatgctgac cttgagggtg tgaattcccg gacttacacg gtatcccatc
    15061 agtggagtaa ttcctacaac cgttccgtca atagaagccg ttgccttgag cgggaaggag
    15121 aagacggtta ggtagcctgg atctgcaggc ggtgtcgtgg tcgtggtcgt ggtcggcgaa
    15181 gtggtcgccg tcgtcgtggc ggtggttgtg gcagtcgtgg tggcggttgc cgttggggac
    15241 gtggttaccg tggttgtcgc ggtcgtggtt gccgtcgtcg tggcggtggt tgtggcagtg
    15301 gtcgtggcgg ttgtcgttgc tgtcgtagtg gctgtcgtgg ttgcagtggt tgttgcggtg
    15361 gtggtcgcgg tggttgttgc ggtggtggtc gcggtggttg ttgcggtggt ggtcgcggta
    15421 gtcgtggcag tcgtggttgc agtggttgtc gtagggggtg taccgataat aatggttccg
    15481 gggaccgtgg ttgcggttat tttcgcaccg ctcccgtcct ggagtgtttt gacagtgatg
    15541 ttcacatcgg tctggccaac ggcatcaccc cttatccgga gaatggcgag tgtgacattc
    15601 gtctgtcccg agttcacctg atacccaaat atcctgattc ccacctcgtc cgccggcgta
    15661 gtagtgtagt aggagaaggg gtaacttgga gcccattctg gaaacaggac atcaaaaatc
    15721 tcggcctttg atggatcggt caacgttgca ttgagactgt atcccagcaa tccatccgga
    15781 aattcatcgg cgacaatcgg gaaatctcgg gtctccccga ctgtcgggat agcgccccct
    15841 tcggcggtta tcgtggtcgc ctgcacgact gaaaagagcg ccccgatcaa aagaatgagt
    15901 gcaatcagtt tcatagtcga atggtaagtc attttcatcc tcctctcttc attctgctcc
    15961 agaaaaaata tcatggcacc tcattgaaca gtagcacgag gtcattaaag tccaaaccgt
    16021 caatcccatc ataattaaag agagcgattg gctggcccgg ttcgctcatc cactcgtcga
    16081 atagcaggaa gaacagcacg acatccacga aatcaatatc gccgcttcca ttcaggtccc
    16141 agtagacccc atcgtgatat ggagccgtcg gtggtccgta tatcggccag ttctctgtcg
    16201 gggggaactg ctgaagcagg acaatctcaa cgtctgccgg ggtggattga gtctgaatgt
    16261 tatctcctgt gtctgcttgc atcagttcta ccgtgacatt aatggtcgtc gatccaggca
    16321 catagcctgt ggcattgaac gttgcgagcg tcacattcgt tgccccgggt ttgatcgcat
    16381 caggcacgtc cgccacgcgg agccagactg atgagccggg cactgtgctg ttcctggtcc
    16441 aagtgggatc agcccaagac ggcgggttca tctcgacgaa ctgggctgct gcggggtttg
    16501 caaagtatac cgtgatgttg tagatgccga gcccctgatc gaacttctca gcctcgagcc
    16561 tcacctgggt ggttgaattg gtcgggatga ggaacgatga cgggacgaag tagagattgg
    16621 tgtaaaacac gttgccagat acttggatgt accttgagcg gggtaaggta tttgtcccaa
    16681 actcgtttcc gacagtgagt gcaacagtgt aattcccgga gttatcatag gtgtgattca
    16741 ccggcatcat agtcgtgttc tcggtcctgt ccaatattgt cccatcccca aagttccact
    16801 gccaccactc gggataaccg gtggaccagt cagtgaagtt gacgagatct ccctcgatga
    16861 cattggtgtt atctgcagtg aagttggcga ccggcaactc ccccacgcgt acgaaatcgg
    16921 tcttcgacat ggtatcgttg ccgtacaggt tgaacacggt gaggttgacc gtatagttcc
    16981 ccttcgccag gaacgtatgg ttcgcgttcc tctgggccgg gaccgtcgta ttgaaccatt
    17041 cgaccgctgt ggtgttgtca aagctccagt tccacgcggt gatattcgga attccgaaag
    17101 agagatctgt gaagaccacg ttgagcggca ccaccccgaa cctgggggtt gcattcgtga
    17161 agtttgcaat cgggacatcg cccgcttcga caaggaagta ttttgtcgtg ctgttgaacc
    17221 cgtactcgtt ggtcacgtta aggctcacgt tgtagaggcc agggatatgg taggtccagt
    17281 tgggatgctg gttgatgctg aagttcccgt ctccgaaact ataattccat gagagagggg
    17341 tatcgtttgt caggtcggtg aaattcacat cgaagggtac ctgaccctgg cagaatgcac
    17401 actccacatc ggacacgttc gccgagaagt tcacgatcag gggcacaccc acgtctatgt
    17461 agaagggttt gctcgtggaa ttcgtccctt
//
LOCUS       DAOQYR010000046        16681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_3875_length_16681_cov_5.780645, whole genome shotgun sequence.
ACCESSION   DAOQYR010000046 DAOQYR010000000
VERSION     DAOQYR010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 16681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16681
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3875_length_16681_cov_5.780645"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="PKM87_06810"
     CDS             complement(<1..905)
                     /locus_tag="PKM87_06810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ29815.1"
                     /translation="MRDPVPVDAYKTRKKPYTILIIDSDESSADRIKKIFSRRKKTFQ
                     LTFTRTLEEAREVLGTVEPALIFAALELPDGRGIEVLPKTDGYATLPLVILADRGNQK
                     DAQAVLKSGAVDYVVKSDVSLEGLPFIAERSMAGWGNLMKRVAAEKAAGESREKFQAL
                     IENITAAIGYYDLEGNTLFYNKAAAGAMGGCPEDFVGKSIYDLFDRALADTIFRRIQA
                     TRKSPEKPGVYEDPVTLPTGTKWFLSLYSPVRNKGGAITGVQIISTDITGLKQAEEEA
                     AQFRKRLADILDFLPDATFAIDAEG"
     gene            complement(917..1372)
                     /locus_tag="PKM87_06815"
     CDS             complement(917..1372)
                     /locus_tag="PKM87_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ29816.1"
                     /translation="MADLPGEPLHILLVEDNEAHAELVIRGMRDQQVANRIHHVIDGE
                     QALDYLFHRGKFADVQKNPSPNLILLDLRLPRIDGLEVLKTLKTTPHLLRIPVVVLTS
                     SDAETDIVQSYDYHANSYLVKPLEFKKFTQLMKDLGFYWLGWNTMPIMD"
     gene            complement(1398..2645)
                     /locus_tag="PKM87_06820"
     CDS             complement(1398..2645)
                     /locus_tag="PKM87_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ29817.1"
                     /translation="MTRVLIADDNQRNLYLLEAIMKGNGFEVVSARNGAEALAAAKRD
                     PPDIIVTDILMPVMDGFELCRRWKADPVLAPVPFIFYTATYTDPKDEQFARSLGAERF
                     LIKPQKPDVLVAEIRQVIEESRSLSGRREAKPQADEKEILQEYSEVLFRKLEKKVMQL
                     EEEIAERKALEEQREAIIRDLEQKNAELERFTYTVSHDLKSPLITIQGFAGLLREDAQ
                     KRDMDRLQEDISRITDAAGKMQDLLTDLFNLSRIGRIGSPYEDISLSTIAREAVHFLS
                     APLAEKRIAVEISPDLPVVHVDHTRIREVYTNLIENSIKFMQEQESPKVRIGMRESVD
                     GPVFFVQDNGMGIDSKYLSKIFGLFEKMDGRSAGTGIGLAIVKRIIEVHGGRVWAESE
                     GPGKGTTICFTLLQEKCGATGKE"
     gene            complement(2642..3034)
                     /locus_tag="PKM87_06825"
     CDS             complement(2642..3034)
                     /locus_tag="PKM87_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ29818.1"
                     /translation="MKRKILYIEDNDQNYYLVSFILNAQGHTVTRARDGREGIDLATR
                     EHPDLILLDIQLPVMDGYATARELRRIPGVAATPIVALTSYAMAGDREKALEAGCTGY
                     IEKPINPKTFTEQIKNYFPAGEATGGGG"
     gene            complement(3031..4947)
                     /locus_tag="PKM87_06830"
     CDS             complement(3031..4947)
                     /locus_tag="PKM87_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ29819.1"
                     /translation="MNLQARVIILFLCIALLVLGVIGGVLPSSLHKQNLNTVAKNTID
                     QLTHVDFALSNFIGSVRYDVNELSINEEVRNPNDASFTNFLNATEEAFEYSIGPEEQE
                     IVDTFRDYQVTHPYVSSVYMGRENGAFVRAYPRARPTEYDPRERPWYILAKDHPGEAV
                     VTDPYMAVTTPDVNIGIVNALLDENGTVYGVVGADITLINLTNYISRFTVGHEGEMIL
                     TDKNGMILASRNTSLLFGNISGILKEQTPGFLASQEGVLVLNGTYLMYYTSPELGWKI
                     GAFVPLSSIEREINNSIAGILIFVILALVLLSAITLLIINYTVIRPLANLTEVSRRIT
                     ETGDLDQEIDTRGAGEIGSLARSFEAMVEKIRTEAQGRQRAIEELEAYRDHLEDIVAE
                     RTRELAQAKEAAESADRIKSAFLATMSHELRTPLNSIIGFSGILLQELAGPLNDEQKK
                     QLGMVSDSAEHLLALINDVLDISKIEAGQLKVACEPFDLRPILEKVARSVRPMAESKG
                     LGMHLDIDPGVGNVVADPRRVEQVTLNLLSNAIKFTDRGDIWIRASTGEQEVTVSVTD
                     TGIGIAPENLDKLFRPFTQIETGLSRQYEGTGLGLSISKRLVNLMGGTIRVDSEPGRG
                     SMFSYTIPTERTAS"
     gene            complement(5064..7157)
                     /locus_tag="PKM87_06835"
     CDS             complement(5064..7157)
                     /locus_tag="PKM87_06835"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNQ29820.1"
                     /translation="MLSGVSMAILGFYGRRFVGRVPAATPYVLIMFAASAWAILYTLE
                     LLSTSLPLKVFYHNLRFLFLPYFAVLELWLVTAYVKKTDWLRLDWALAILVIPVLSSV
                     LALASPLHSLFRYNFFLNSTGPMPVLQYTEGVFFIFYSMFSFILLAAAILILINESRK
                     SRTVWEVQTLLLLVALAFPTVINYLWTAGITPVPGVNMAPALLWIPAILYTVALFRYR
                     FLDIIPVARSRIIETLNAPVIVLDTEGRIIDLNPAASPLLTTSLEPALGKKISDLASL
                     HPDLLILCVSETETRTELTWNTGGSSQHFQGSVEILRTPGGQPEGRLILLQDITDLKH
                     TEQSLRESEEKFSKAFSSSPYAITITRMDDGLVLDVNDGFARITGYSPPEVVGKKSPD
                     LQLWADTGDRDAVVRDLREGTRVYSREYRFRTKGGRTITGLFSADIIVVQGREYILSS
                     INDVTDKKIAEEQRENLIRELKLRNVELDRFTHTVSHDLRNPLFTIKGFLDMLKEDME
                     AGDFTRANADISKISIAADKMEELLTTLLALSRSGKSIDSPLPVSFSDVAREAAELLD
                     ATIRSHNVDLVIHGNLPTVMGDRQRLLQVMLNLIDNAVKFMGDQEEPRVEVGVRTEGP
                     DTLFFVRDNGIGIEPKDINSIFGLFTRLDTGVPGSGIGLATVKRIIEVHGGRVWAESE
                     GPGKGTTICFTVPEP"
     gene            complement(7204..11103)
                     /locus_tag="PKM87_06840"
     CDS             complement(7204..11103)
                     /locus_tag="PKM87_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ29821.1"
                     /translation="MSKVTPVDESQKGTRIWLFTGAALLILLILAAGVIFYQSQEKQA
                     RETVTGELVSISTLKADEIARWREDRLHDARTLSASGFFIEGVDDYLSSPDPESEEKI
                     LERFREVNESVHYHNVLLVDPGGTIRISLDPNTTTLAPEGLTALKESLQTGEAFMTDL
                     HLLPGTSHPHIDMVAPLLVESDGKEEPVGAVILSIDPERFLYPLVQSWPVPSRSAETL
                     LVERQGDHVLFLNDLRFQQHTALNLTIPLSETEFPAVMAVLGETGAFEGTDYRGVEVI
                     SVLEPVRGTPWHIVAKVDRGEAFSGWAAQSLLIFSLAAGILASVLVVTGWAWQRRQKH
                     YYRSLYRTEAGRAREEQVNRERLDALVHISEMEAASEKEIADYVLDAACHLTGSSLAF
                     IGSMSPDETVFHLAAWSSSVMEECAVAGVPLHFPIAEAGIWAEAVRTKKPVILNDYPA
                     PHPAKKGFPAGHVQITRFAAVPVLDGERVVMVSAVANKEADYTETDIDQMMLLMQGFW
                     THLQRHAARDALERKRTDLEAAYEEITATQEELQANYEELAKSQIALRESERKYRDLY
                     EYAQVGLFETSLEHATVIACNQRFCELFGFPSASEAIGKDVLALYVDPSDREEIGRIL
                     KEKGAIEYHVVRFRNQSTGRLFWGQFSALYNRERDIIEGSIVDITAQKAAEARLRETN
                     EYLDNLINYANAPIIVWDPGFRITRFNNAFERLTGCTSDTVTGKPVSVLFPERTRERS
                     MEYLHQAMAGERWETVEIPILRRDGSERVVLWNSATLYSEDGKTVIATIAQGQDITGR
                     KAAEQALKESEERYREFFTTSRDSVFITTPEGKWLDFNDTSLEMFGYPTRDELMALPV
                     PQLYADPLERDEFLAVIAREGFVKEHPMRLRRKDGTVIDTLITAVPLRDRENAQKAFM
                     GTIRDVTFRKAAEKALRRSEDTFRHISGLITDFAYSCRKSPGGAFCIDWLTGAVETIT
                     GYTQGEIHILTCWRPLVIEEDLPVFDQNVVGLLPGESSRSEIRIRHRDGRIVWLASFA
                     ECVTDPEDPTFHRLYGGCRDISARKEADRRQNALVRELEQKNAELERFTYTVSHDLRS
                     PLITIRGFAELIDEDAEKGDLPRLHDDLKRIISAADRMQDLLNDLLELSRIGRLVNPS
                     EKVRLDIIAQEAADLLAGPIGNAGVEVDISPDLPVVNVDRTRMREVFTNLIENAIKFT
                     RGQEHPLIQIKMREEGGEKIFFVQDNGIGIEPRYLSKIFGLFEKLDGRSEGTGIGLAI
                     VKRIIEFHGGRIWAESEGPGKGTTICFTLPVLDEGAA"
     gene            11604..13433
                     /locus_tag="PKM87_06845"
     CDS             11604..13433
                     /locus_tag="PKM87_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ29822.1"
                     /translation="MPTARIMVVEDEQIVAEDLRHVLNGMGYRVVGIAGSGEDAVRMA
                     RETMPDLIMMDISLAGPMDGTEAAGKIAAFHDAPVIYLTAFADSAIIEKAKVTRPYGY
                     ILKPYQEREIRTAIEISLYTHDLHRKIAESEERYRGFVQNFLGIAYRRRPDFSPEFFQ
                     GAVEAISGYTEAELLANTPAWEDLIHPADADRIRLHNIEISQTGSGSATRDYRIAKRD
                     GKIAWVHELIRVIPEIDGSTHAILGSIYDITEQKAAEDALQAMNQELEERVRQRTLSL
                     HQQVEFLQQLIDTIPSPIYYKNPSCVYLGCNAAFESYAGLSRSAIVGRTDNEIFPPDF
                     AALTREKDRFLLDHRGIQAYQAKFPHADHFTRHILVKKATYHDSEGKIAGFIGVLVDI
                     TDRIRAEEQVRESERRFRALVQDQTDLIFRFLPDRTILFANEAFLSYFGKSSEDVTGF
                     IFRLPIHPDDLEDVQAHFASLSPPHPVSTTEFRCLRNGDEIRWQQWIDRAFFDERGTV
                     VEYLSVGRDITEKKEIERREREAYQQIEKNMQQFATLNDQIRNPLSVIVMLAGFGSDE
                     NSQKILERARDIDSILDRLDTGWQESEKVRKFLKKHYGIGGGE"
     gene            complement(13477..14247)
                     /locus_tag="PKM87_06850"
     CDS             complement(13477..14247)
                     /locus_tag="PKM87_06850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ29823.1"
                     /translation="MVQKKVMIVEDEGVVALSLKSTLSKMGYTITGIAITGKEAITLA
                     LETQPDVILMDIHLKGDIDGIQTTEELNRVTDIPVIYLTAYADEETLSRALKTESHSY
                     LVKPFNPRELYSNIEFAIYKRRLKEKIGSAREKLELHLTRSPDCAYIIDLHNRIVFAN
                     PSSEILTGYGAEEVAGKNVFALLNIAPSKKEGSGDDTLQRVLSLDAMYYLPSLALITM
                     KSGKMRTVSLKAGIIREEGAEAKNLLLLMKDASSPDLS"
     gene            14405..16450
                     /locus_tag="PKM87_06855"
     CDS             14405..16450
                     /locus_tag="PKM87_06855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ29824.1"
                     /translation="MREISRMNLAIFLITAVITFAIVLTTIFSLKSGIYDIFPYFYII
                     PVILIAYSNPRLGVYFTIVLGWIYLSLVYLYGPFDIRLYATSSAWFYIFITLGVVISA
                     FSNEIAKERKFRDIFLNSQAGIFTFRPGDQLIRVANTQVASLLGYTPEEMQNLHIADI
                     IPDTEEMSGFFQKLENEGKISDAEVTLQKKDGSRVWVLITASRTSGDQVICSAVDITE
                     RKRIKDELRYTEIHYRALFDSAGDAIIIHDFEGNIFEANAIASEQSGYSRQELEQMHL
                     SDLDILPAREAAGTWVQEIRKKGISIRETLLKTREGATIPVEISSKVIEYYRSPAVIT
                     IIRDLRARKEAENALRESERRYRMIGDLIPFGVWITDAGGTLTYASQSFLDLIGMTLD
                     ECRRNGWMKKLPVEQRESTRNDWKQCVETGCFWDYEYRIVDRQGTEHFILSRGAPMRD
                     ASGRIVSWVGIHFDISQRRRYENRLEASLREKEVIIKEVHHRVKNNMQVISGFLQLQT
                     NYLTDQESIEKLEECQMRVKTMALVHEKLYQSKYLGFINTRDYIQSLISDLMNSYLLR
                     SDISIEQDIDDVNINLDTAIPCGLILNELLTNALKYAFNGRESGTISVSLHLNPDHRF
                     SLDISDDGVGLPPGLDIAQAPTLGLQLVTVLVRQIGGEMQVGGPPGTSFHISFPEKF"
     gene            complement(16475..>16681)
                     /locus_tag="PKM87_06860"
     CDS             complement(16475..>16681)
                     /locus_tag="PKM87_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29825.1"
                     /translation="SLMYDGEPGDSLDMDMHGICPSVMKGYDRDEIEPIVSFQAPPPK
                     RPGEGYAGVGLMKIGVQSLRGKAP"
BASE COUNT         3437 a         4809 c         4681 g         3754 t
ORIGIN      
        1 cttccctccg cgtcgattgc aaaggtcgca tcggggagga aatcgaggat atctgccagg
       61 cgttttcgga actgcgccgc ttcctcctcg gcctgtttca agccggtgat gtcggtggag
      121 attatctgca caccggtgat ggcgcccccc ttatttctga ctggcgagta tagcgagaga
      181 aaccacttgg tcccggtggg aagagtgact gggtcttcgt acacccctgg cttttctgga
      241 gatttccttg tcgcctgaat cctccggaaa atcgtatcgg caagagcccg gtcaaagagg
      301 tcgtaaatcg atttccctac gaaatcttcg gggcagccac ccatcgctcc cgccgcggcc
      361 ttgttgtaaa aaagtgtgtt tccttcgaga tcataatagc cgattgccgc ggtgatattc
      421 tcgatcaggg cctgaaattt ttccctggat tctccggctg ccttttcggc tgccacccgc
      481 ttcatgaggt tgccccatcc cgccatggag cgttcggcaa tgaatggcaa cccttcgagg
      541 ctcacatcag attttaccac gtagtctact gcgccagatt tcagcaccgc ctgtgcatct
      601 ttctgatttc cgcggtcagc caggatcacc agggggaggg ttgcataccc gtctgttttt
      661 ggcaggactt cgatcccacg gccgtccgga agctccaggg ccgcgaagat gagggcgggt
      721 tcgacggtac cgagcacttc ccgggcttct tcgagggtgc gggtaaaagt cagctggaat
      781 gtctttttcc gcctggaaaa gattttcttt atacggtctg ctgatgactc gtcggagtcg
      841 ataataagaa tagtataagg tttctttctt gtcttgtatg catccaccgg aacaggatcc
      901 ctcataatac cgggctttag tccataatgg gcattgtatt ccatccaagc cagtagaagc
      961 ccaggtcttt catcaactgg gtgaatttct tgaattccag gggtttgacc aagtaactgt
     1021 tcgcatggta atcatatgac tgtacaatat cggtctcggc atcagaactt gtgaggacga
     1081 caacaggtat tctgagaaga tggggggtcg tttttaaggt cttgagtact tcaagcccgt
     1141 cgatccgcgg caaacggaga tcgagaagaa tgaggtttgg ggaggggttc ttctgcacat
     1201 cagcgaattt accgcggtgg aagaggtagt cgagggcctg ctcgccgtct atcacgtggt
     1261 ggatcctgtt ggccacctgc tggtcccgca taccccgtat caccagttcg gcatgcgcct
     1321 cgttatcttc gacaagaagg atgtgcaggg gttcccctgg taggtctgcc atgaaaaaca
     1381 gttctcactc tgtattctta ttctttgcca gtcgccccgc acttctcctg gagcagggtg
     1441 aagcagatcg tcgtgccctt tcccggtcct tccgactctg cccagaccct gcccccatgc
     1501 acctcgatga tccgcttcac gatggcaagg ccgatgcccg ttcccgcgga ccgcccgtcc
     1561 atcttctcga acaggccgaa tatcttcgag agatacttcg aatcgattcc cataccgttg
     1621 tcctgcacga agaagacagg tccatccacg ctttcccgca ttccaatccg aaccttcggg
     1681 gattcctgtt cctgcatgaa ttttatcgag ttctcgatga ggttggtata gacctcccgg
     1741 atgcgcgtgt gatcgacatg cacaacggga aggtcagggg atatctcgac agcgatcctc
     1801 ttttcggcaa ggggcgcgga gaggaagtga accgcttccc tggcaatcgt gctgagggat
     1861 atatcctcat acgggctgcc gatcctgccg atgcgggaga ggttgaagag gtctgtcagg
     1921 aggtcctgca tcttcccggc cgcatcggtg atccggctga tgtcctcctg gaggcggtcc
     1981 atgtctcttt tctgtgcatc ctccctcagg agcccggcaa acccctggat cgtaatgagc
     2041 gggcttttta agtcatgcga gacagtgtag gtgaaacgtt cgagctcggc gttcttctgc
     2101 tccagatccc tgataatcgc ttccctctgt tcttccaggg cttttcgttc agcaatctct
     2161 tcctccagct gcatcacctt cttttcgagt ttccggaaca gcacctcgct gtactcctgc
     2221 aggatctctt tctcgtctgc ctgcggcttt gcttcccttc tcccgctcaa cgagcgtgat
     2281 tcctctatca cctgcctgat ctccgcaaca aggacatccg gtttctgggg cttgatgagg
     2341 aaccgctcgg ccccgaggct ccgggcgaac tgctcgtcct ttggatcggt gtatgtggcc
     2401 gtgtaaaaaa tgaaagggac aggggcaaga acagggtccg ccttccacct gcggcagagc
     2461 tcgaacccgt ccatcaccgg catcaggatg tcggtgacga tgatatcagg ggggtctcgc
     2521 tttgcagcag cgagtgcctc ggcgccgttc cgcgcagata ccacttcaaa gccgttccct
     2581 ttcatgatgg cctccaggag gtagaggttc ctctggttat cgtccgcaat caggacccgc
     2641 gtcatcctcc tcctcccgtg gcctccccgg ccggaaaata attcttgatc tgttccgtga
     2701 atgtctttgg gttgatgggt ttctcgatgt atcccgtgca ccccgcttca agcgctttct
     2761 cccggtctcc ggccattgca taggaggtga gggcgacaat gggggtcgcc gcaacgccgg
     2821 ggatacgccg gagttcccgg gcggtcgcat acccgtccat gacggggagc tggatgtcga
     2881 gcaggatgag gtcagggtgt tcacgcgtcg cgagatcaat cccttccctt ccgtcccggg
     2941 ccctcgtcac tgtgtgcccc tgtgcattca ggataaagct gaccaggtag tagttctggt
     3001 cattgtcctc gatatagaga atttttctct tcatgatgca gtcctttccg tcgggatggt
     3061 gtaactgaac atgctccccc tgcccggctc gctgtcgacc cggatggtcc ctcccatgag
     3121 gtttacgagc cgctttgaga tcgagagtcc aagccccgtc ccttcgtatt gcctcgagag
     3181 tccggtctcg atctgggtga agggccggaa cagcttgtcc aggttttcag gtgcgatccc
     3241 gatacctgta tccgtcacac tgacagtcac ctcttgctcc ccggttgacg cacgtatcca
     3301 gatatcccct ctatccgtga acttgattgc attggagagc aggttgaggg tgacctgctc
     3361 gaccctgcgc gggtctgcca ccacgttccc cacgccggga tcgatatcga ggtgcatccc
     3421 gagacccttg ctttctgcca tgggccggac actccgtgcg actttttcga gaatggggcg
     3481 gaggtcaaac ggttcgcatg cgaccttgag ctgcccggcc tcgatcttcg aaatatcgag
     3541 cacgtcgttg atcagggcaa gcaggtgctc ggcgctgtcc gagaccattc caagctgctt
     3601 cttctgctcg tcattcagtg gcccggcaag ctcctggaga aggatgcctg agaacccgat
     3661 gatggagttc agcggggtcc gaagttcgtg cgacatggtg gcaaggaatg cagacttgat
     3721 ccggtctgcg gattctgccg cctcttttgc ctgcgcaagc tcgcgggtcc gctcggcaac
     3781 aatgtcctcg aggtgatcac ggtatgcctc aagctcctcg attgctctct ggcgcccttg
     3841 agcctcggtg cgtatctttt cgaccattgc ctcgaaggaa cgtgcaaggc ttccaatctc
     3901 gccggcaccg cgcgtatcga tctcctggtc gaggtctccc gtctcggtta tcctcctgct
     3961 cacttctgtc aggttcgcga gcggccggat gaccgtgtag ttgatgataa ggagggtgat
     4021 cgcactgagc agcaccagcg ccaggatgac gaagatgagg attcctgcga tcgagttgtt
     4081 gatctcccgt tcaatgctgc tgagcgggac gaacgcacct atcttccatc caagttcggg
     4141 cgaggtataa tacataaggt aggtcccatt gaggaccagc accccttcct ggctggcaag
     4201 aaatccaggg gtctgctcct tcaggatccc gctgatattt ccaaacagca gggacgtatt
     4261 ccttgaggcg agtatcatgc cgtttttatc ggtgaggatc atctcacctt cgtgtcccac
     4321 ggtgaagcgg gagatgtaat ttgtcaggtt tataagcgtg atatcggcgc cgacaacccc
     4381 gtatacggtc ccgttctcgt caaggagcgc gttcacgatc ccaatgttga catccggcgt
     4441 ggtgaccgcc atgtaagggt ccgtcaccac ggcctccccg gggtggtctt ttgccagtat
     4501 gtaccagggg cgttcccggg gatcatattc agtaggcctg gcccgcggat acgcacggac
     4561 aaacgcaccg ttctcccttc ccatgtagac cgaactgacg tacgggtgcg tcacctggta
     4621 atcgcggaag gtatcgacga tctcctgttc ctctggaccg atggaatact cgaaagcctc
     4681 ttccgttgca ttcaggaaat tcgtgaagga tgcgtcgttg ggattcctga cctcctcgtt
     4741 gattgagagc tcgttgacat cgtacctgac gctcccgatg aaattggaga gtgcgaaatc
     4801 gacgtgcgtg agctggtcga tagtgttttt tgcaacggta ttgaggtttt gcttatgcag
     4861 ggatgacggc agcacccctc cgatgactcc aagaacaagg agtgcgatgc acagaaagag
     4921 gatgatcacc cttgcctgga ggttcacaat gactggcggt ttaatttata gaataaaaag
     4981 gtttgcgcag ccacatgaat tgagaccgga tgaccttttg cggggttcta tctcttttac
     5041 acctgagcgc tatccgtggt aatctagggt tcaggcacag tgaagcagat cgtcgtgcct
     5101 tttcccggcc cttccgattc cgcccagacc ctccccccgt gcacctcgat gatccgcttg
     5161 accgtcgcaa gcccgatccc gctgcccggg acacccgtat caagccgcgt gaagagtccg
     5221 aaaatgctgt tgatgtcttt tggctcgatg ccaatcccgt tatcccggac aaaaaataag
     5281 gtatccgggc cctctgtacg cactcccacc tcaactctcg gctcttcctg gtcgcccatg
     5341 aacttgacgg cattatcgat gagattgagc atcacctgca gcaggcgctg ccggtcgccc
     5401 atgacggtgg gaaggttgcc gtgtatcacg aggtccacat tatggctgcg gatggtggca
     5461 tcgagcagct ctgccgcttc ccttgcaaca tcggagaaag agaccgggag cggcgaatcg
     5521 atgctttttc cgctccggga aagggcaagg agcgttgtga gcagctcttc catcttgtct
     5581 gcggcgattg aaatttttga aatgtcagcg ttcgccctgg tgaaatcgcc cgcttccata
     5641 tcctccttca gcatgtcgag gaaccctttg atggtgaaga gagggttccg caggtcatgg
     5701 gagaccgtgt gggtgaacct gtcaagctcg acattcctga gtttgagttc ccggatgagg
     5761 ttctcccgct gctcttctgc aatttttttg tcagtgacgt cattgatgct ggagaggata
     5821 tactccctgc cctgcacgac gatgatatcg gccgagaaga ggccggtgat ggtccggccc
     5881 ccttttgtcc tgaaacggta ttccctgctg tacacccttg taccttcacg gaggtcacgc
     5941 accacggcat cacggtcccc cgtgtcggcc cataactgaa ggtcgggact cttcttcccc
     6001 accacctccg gcgggctgta accggtaatg cgcgcgaacc cgtcgttgac atccagtacc
     6061 agcccgtcat ccatccgggt aatcgtgatc gcgtacggag aggagctgaa cgccttcgaa
     6121 aacttctcct cgctctcgcg gagcgactgc tcggtatgct tgagatcggt gatgtcctgc
     6181 agcaggatca gcctgccttc cggctggccg cccggtgtgc gaagtatctc gactgacccc
     6241 tggaaatgct gcgatgatcc ccccgtattc caggtgagtt cggtgcgggt ttctgtctcc
     6301 gatacacaca gtatgagcag gtctggatga agcgatgcaa ggtcgctgat cttcttccca
     6361 agtgcaggct caagggaggt tgtgagcaac gggctggctg cagggttcag gtcaatgatc
     6421 cggccctctg tgtccagcac gatgaccggg gcattcaatg tctcgatgat cctgctgcgt
     6481 gcaacaggga tgatgtcaag gaaccggtac ctgaagagtg caacggtata gaggatggca
     6541 gggatccaga gcagggcagg ggccatgttc actccgggaa ccggggttat gcctgcggtc
     6601 cagagatagt tgatcacggt gggaaaggcg agggcaacga gcaggagcag ggtctggacc
     6661 tcccacaccg tgcgggattt cctgctctcg ttgatgagga tcagtatcgc tgcggcaagc
     6721 aggataaagg aaaacatgga ataaaatatg aagaaaaccc cctcggtata ctggaggacc
     6781 ggcatcggtc ctgtgctatt caggaagaaa ttgtaccgga agagcgagtg gaggggactg
     6841 gcgagggcga gcacggatga gaggacggga atcacaagta tggccagtgc ccagtccagg
     6901 cgaagccagt cggtcttctt cacgtaggcg gtgaccagcc acagttcgag aacagcaaag
     6961 tatggcagga acaggaaccg gaggttgtga taaaagacct tgaggggaag cgaggtcgag
     7021 aggagttcaa gcgtgtacag gattgcccag gcagatgcgg caaacatgat gagcacatag
     7081 ggagtggcag cagggaccct tccgacaaac ctcctcccat agaatccgag gatggccata
     7141 ctcactcccg acaacatcag catcaagacg atgaagtcta atatccacat gttccaggtc
     7201 cttttaggcc gctccttcat ccagtaccgg gagagtgaag cagatcgtcg tgccttttcc
     7261 cggcccttcc gattccgccc atatccttcc gccatggaac tcgatgatcc gcttcacgat
     7321 ggcaaggcct atgccagtcc cctctgatcg cccgtccagc ttctcgaaga ggccgaagat
     7381 cttcgagagg taacgcggct cgatccctat cccgttgtct tgcacgaaga agatcttctc
     7441 tcctccctct tccctcattt tgatctgtat gagggggtgc tcctgccccc tcgtgaactt
     7501 gatggcgttc tcgatgaggt ttgtgaatac ctccctcatg cgggtgcggt ccacgttgac
     7561 aacggggaga tcgggagaga tatctacctc cactcccgcg ttcccgatgg gcccggcgag
     7621 gaggtctgcg gcctcctgtg caatgatgtc tagccggact ttttcggatg gattgacgag
     7681 ccgtccaatc cttgagagtt cgaggaggtc attgaggagg tcctgcatcc ggtcagcagc
     7741 gctgatgatc cttttgaggt catcgtgcag gcggggcagg tcacctttct ctgcatcctc
     7801 atcaatgagt tctgcaaatc ccctgatggt gatgagcggg cttctcaggt cgtgcgagac
     7861 ggtgtaggtg aagcgctcaa gctcggcatt cttctgctcg agttccctga caagtgcgtt
     7921 ttgcctccgg tccgcctcct tccttgcact gatgtccctg cacccgccgt agagccggtg
     7981 gaaggtgggg tcttcgggat cggtgacgca ctcggcaaac gaggcgagcc agacaatcct
     8041 cccgtcgcga tggcgtatcc ggatctcgga ccgggacgat tctcccggca gcaggccgac
     8101 cacattctgg tcaaagacag gaaggtcttc ttcgatgacg aggggacgcc agcatgtcag
     8161 gatatggatc tccccctggg tataacccgt gattgtctcc accgcgccgg tcagccagtc
     8221 gatacagaag gcccctcccg gggatttcct gcaggaatat gcaaagtccg tgatcagccc
     8281 ggatatatgg cggaatgtat cctcgctccg tcgaagtgcc ttttcagccg ctttccggaa
     8341 cgtgacatcc cttatggttc ccatgaacgc tttttgcgcg ttttcccggt cgcggagggg
     8401 gactgccgtg atcagggtgt cgataacggt tccgtccttc ctcctcaacc gcatggggtg
     8461 ctctttgaca aaaccctccc gcgcgatgac tgcgaggaac tcgtccctct ccagtggatc
     8521 cgcgtagagc tgcgggaccg gcagcgccat cagttcgtcc cgggtcgggt acccgaacat
     8581 ctcgagcgat gtatcgttga agtccagcca tttcccttcc ggggtcgtga tgaacaccga
     8641 gtcccgcgat gtggtgaaga actcccggta ccgctcctcg ctctccttta atgcctgttc
     8701 cgctgctttc cgcccggtga tgtcctgccc ctgggcgatc gtcgcgatca cggtcttccc
     8761 gtcttcgcta tagagcgtcg ccgagttcca caggacgacc ctttcactcc catctctccg
     8821 gagaatcggg atctccacgg tttcccatcg ctcgccggcc atcgcctggt gcaggtactc
     8881 catcgagcgc tcccgcgtcc gttcagggaa gagtacgctg accggctttc cagtcacggt
     8941 atccgatgtg cagccggtga gtcgttcgaa cgcattgttg aaccgggtga tccggaatcc
     9001 tgggtcccag acgatgatcg gggcgttcgc gtaattgatc aggttgtcga ggtactcgtt
     9061 ggtctcccgg agcctggctt ccgccgcttt ctgcgccgtg atatcgacga tcgaaccctc
     9121 gattatgtcc ctctccctgt tgtaaagggc tgagaactgc ccccagaaca gtcgccccgt
     9181 cgactggttc ctgaacctga ccacgtgata ttcaatggcg cccttttcct tcaggatcct
     9241 gcctatctct tccctatctg aaggatcgac gtagagcgcg agcacatcct ttccgattgc
     9301 ctcgctggcg gagggaaagc cgaacagttc gcagaaccgc tggttgcagg cgatcacggt
     9361 ggcatgttca aggctggttt caaagagccc gacctgggca tactcgtaca ggtcccggta
     9421 tttccgttcg ctctcgcgaa gtgcgatctg gctctttgcg agctcctcgt agtttgcctg
     9481 gagttcctcc tgcgttgcag tgatctcctc gtaggcagcc tcgagatcgg tacgtttcct
     9541 ctcgagcgca tctctcgcgg catgcctctg caggtgcgtc cagaatccct gcatgaggag
     9601 catcatctga tcgatgtcgg tctctgtgta atcagcctcc ttgtttgcca ctgcgctgac
     9661 catcacaacc cgctccccgt caaggacagg gactgctgcg aacctggtga tctggacatg
     9721 cccggcagga aaccctttct ttgccgggtg cggagccggg taatcgttga gaatgaccgg
     9781 cttttttgtc cggaccgcct cagcccagat gccggcctct gcaattggga agtggagggg
     9841 caccccggcc accgcacatt cttccatgac cgaactcgac caggccgcca ggtgaaagac
     9901 tgtctcgtcc ggggacatgc tcccgatgaa cgcaagcgaa ctccccgtaa gatggcatgc
     9961 agcgtccaat acgtaatcgg caatctcctt ctcgctcgcg gcctccatct ccgagatatg
    10021 cacaagggca tcgaggcgtt cgcggttgac ctgctcttct cttgcccttc cagcctccgt
    10081 gcgatacagc gaacggtagt agtgtttctg cctccgctgc caggcccagc ctgtgacgac
    10141 cagcacgctc gcaagaatcc cggcggcgag tgagaagatg agcagggact gcgctgccca
    10201 ccccgagaat gcctccccac ggtcaacctt tgcgacaatg tgccaggggg tgccacgcac
    10261 aggttccagc accgagatga cctcgacccc gcggtaatcg gtgccctcga atgcccctgt
    10321 ctccccaagg accgccatca ccgcgggaaa ctcggtctcg ctcaggggga tggtgaggtt
    10381 gagcgccgtg tgctgctgga aacgaaggtc attgaggaaa aggacgtgat cgccctgccg
    10441 ttcgaccagc agtgtctcgg cgctcctgct cggaaccggc catgactgga caagcgggta
    10501 caggaaccgt tcgggatcga tcgagaggat gactgctccg accggctctt cctttccgtc
    10561 actctcaacg aggaggggcg cgaccatgtc aatgtgcggg tgggaggtgc cgggaaggag
    10621 gtggagatcg gtcatgaacg cctcccctgt ctgaagcgat tctttcaggg cggtgaggcc
    10681 ctccggggca agggttgtgg tattgggatc gagactaata cgaatggttc ccccggggtc
    10741 tacaaggagg acattgtggt agtggaccga ttcgttgact tccctgaacc gttcaaggat
    10801 cttctcttcg ctttccgggt ccggagagga gagataatcg tctacaccct cgataaaaaa
    10861 cccgcttgcg gagagagtcc gggcatcgtg cagcctgtct tccctccacc gcgcaatctc
    10921 gtcagccttc agggtgctga tcgagacgag ttcccctgtg acagtctctc ttgcctgctt
    10981 ttcctgcgac tggtaaaaaa ttacccccgc ggcaaggata aggaggatga gcaatgcagc
    11041 accggtgaaa agccagatgc gcgtcccttt ctggctctcg tcaacgggtg tcaccttgct
    11101 cacgctccct gtacgggtgt gcctgaaaga tcggtgcggc ttttctttcc cccgcctccc
    11161 gggagagccc ggttcgcatc ccaggcggcc atcatgctga cggggaccgc tggcattcct
    11221 cccccacctg ctctaaaaga ccttcgcccc taaggatggt ggcagtccgg tgaaacatcg
    11281 catttgctcc tctgtaaagg agtgcaataa aaaaccctcg cgactggagg aagagtcctt
    11341 ccgtttccca tcgcgtttcg ccagagacat aaaagatatg gagactgctg gcgggcatcc
    11401 ggggttccat gcgaatatca tcaatgactg agaactgctg atatataagt atagagattt
    11461 gcaggggaag gacggaaatt ccggctctgc accgtccatc cgggcccggt ttctgcctat
    11521 cccctgaatc tccctgttcc gacgtactcc aagagaattg ttgatataca cggtgcacaa
    11581 atgaaccaga taacgaattc tccatgccca ctgcacgaat aatggtcgtc gaggacgaac
    11641 agattgtagc cgaagatttg aggcatgtgc ttaacgggat gggatacagg gtggtgggga
    11701 tcgctggttc gggcgaggat gcagtccgca tggcccgcga gacaatgccg gacctcatca
    11761 tgatggacat ctcccttgca ggccctatgg acggcaccga ggctgccgga aagatcgcgg
    11821 cattccacga tgctcctgtc atctacctca ccgcattcgc cgattccgcg atcatcgaga
    11881 aggcaaaggt gacccgcccg tacgggtaca tactgaaacc ctaccaggag cgggagatca
    11941 ggaccgcgat cgagatatcg ctctatacgc acgacctcca caggaagatc gccgagagcg
    12001 aggaacggta ccgcggattc gtccagaact tccttggaat cgcctaccgg aggaggcctg
    12061 acttttcccc ggagttcttt cagggggccg tcgaggcaat atccgggtat acggaagcag
    12121 aactgctcgc gaatacgcct gcgtgggaag acctgatcca ccctgccgat gcggaccgca
    12181 tcaggctgca caacattgag atctctcaga cgggatcagg gagtgcgacg agggactacc
    12241 gtattgccaa aagagacggg aagattgcct gggtgcacga attgatcagg gtaatcccgg
    12301 aaatcgacgg ctcaacgcac gccatcctcg gttctatcta cgatatcacc gagcaaaaag
    12361 cagcggagga tgcgctccag gctatgaacc aggagctgga agagagggtg aggcagcgga
    12421 ccctctctct ccaccagcag gtggaatttc tccagcagtt gatcgatacc atcccaagcc
    12481 cgatctacta taaaaatcca tcctgcgtct atcttgggtg taatgctgcg tttgagagct
    12541 acgccgggct gtcgagatcg gcgattgtcg gcaggaccga caacgagata ttcccccccg
    12601 acttcgcggc gcttaccagg gaaaaggacc ggttcctcct ggaccaccgt gggatacagg
    12661 cataccaggc aaagtttcca catgccgacc atttcacgcg tcatatcctt gtaaaaaaag
    12721 caacctacca cgacagcgag gggaagatcg ccggcttcat aggggtgctc gtggatatca
    12781 ccgacaggat acgggccgaa gagcaggtgc gggagagcga gaggcggttc cgggcactgg
    12841 tccaggacca gaccgacctg atcttcaggt tcctcccaga caggaccata ctcttcgcga
    12901 acgaggcttt tctctcgtac ttcgggaaga gttccgagga cgttacaggg ttcatcttcc
    12961 gcctcccgat acacccggat gacctggagg acgtgcaggc ccatttcgcc tccctctccc
    13021 ctccgcaccc agtcagcacc accgaattcc ggtgcctgcg aaacggagat gagatcaggt
    13081 ggcagcagtg gatcgacagg gcattcttcg atgaacgggg gacagtcgtc gagtacctct
    13141 ccgtagggag ggacatcacc gagaagaaag agatcgagcg gcgcgagcgc gaggcctacc
    13201 agcagatcga gaagaacatg cagcagtttg ccaccttaaa cgaccagatc aggaacccgc
    13261 tctccgtgat cgtgatgctg gcagggttcg ggtcagacga gaattcccag aagatcctgg
    13321 agcgggcaag ggatatcgac tcgatcctcg accgccttga caccggatgg caggagtcag
    13381 aaaaggtcag gaaattcctc aagaagcatt acgggatcgg cggaggcgag tgagggggca
    13441 atccatgcgg ataccctgga tagtaccttc acatatttag ctcagatcag gtgacgatgc
    13501 atctttcatc aggagaagca ggttcttcgc ctcggcaccc tcttccctga tgatccctgc
    13561 tttcagggag acagttctca tctttccgct cttcatggtg atcagcgcca gcgacggcag
    13621 gtaatacatg gcatctagcg agaggacccg ctggagagtg tcatctccac tcccttcctt
    13681 cttcgatggt gcaatgttca gaagggcgaa cacgttcttt cctgcaacct cctcggcccc
    13741 gtatcccgtg aggatctccg aggatgggtt cgcaaagacg atgcggttgt ggaggtcgat
    13801 gatgtacgcg cagtcggggc tcctggtgag atgcagctcg agtttctccc gtgcgctccc
    13861 gatcttctcc ttcagccgcc gcttgtaaat cgcgaactca atgttggagt acagttcccg
    13921 cgggttgaat ggcttcacca ggtagctgtg gctctcggtt ttaagcgcac ggctgagtgt
    13981 ctcttcatcc gcatacgcag tcaggtagat cacggggatg tcagtgaccc ggttcagctc
    14041 ctctgtcgtc tggataccgt caatgtcccc tttgaggtgg atatccatca ggatcacgtc
    14101 aggctgggtc tcgagtgcca gtgtgatcgc ctctttcccg gtgatcgcga tgccggtgat
    14161 agtgtatccc atcttcgaga gcgtcgattt caacgagagc gccactaccc cctcatcctc
    14221 gacaatcatc actttcttct gcaccatggc gtgaactcct gtacgagata gtctcttgcg
    14281 gatgagatag ataaaatgga cgtttccttc accaggtatc cgtgggtaat tttatcagca
    14341 cagaacccgc gatgaagaat aaggcattct gcatctgttg cggtgtacac aaggagcgcg
    14401 gggcatgaga gagatttcaa ggatgaacct ggcaattttc ctgatcaccg cggtgatcac
    14461 ctttgcgatt gtgctgacca ccatcttcag cctcaaaagc gggatctacg atatcttccc
    14521 ctacttctac atcatcccgg tcatcctgat cgcatactcc aaccccaggc tgggcgtcta
    14581 tttcacgatt gttcttggct ggatctatct ctcccttgtg tacctctacg gccccttcga
    14641 tataaggctc tacgcgacca gcagcgcctg gttttacatc ttcatcacgc tcggcgtggt
    14701 gatctctgca ttttcaaacg agattgcaaa agagcggaaa ttccgcgata tcttcctcaa
    14761 ctcgcaggca ggtatcttca ccttcaggcc gggggaccag ttgatcaggg tagccaatac
    14821 ccaggtggct tccctccttg gatacactcc agaagagatg cagaatcttc acatcgcaga
    14881 tatcataccc gacaccgaag agatgagtgg attcttccag aagctcgaga acgagggcaa
    14941 gataagcgat gccgaagtca ccctgcagaa gaaggatggc agcagggtat gggtcctgat
    15001 caccgcgtcc cggacctcgg gggaccaggt gatctgctcg gccgttgaca ttaccgagcg
    15061 gaagcggatc aaggacgagc tccggtacac cgagatccat taccgcgccc tcttcgacag
    15121 tgccggggat gccatcatca tccacgactt cgaagggaat atcttcgagg caaatgcaat
    15181 cgcgagcgag cagtctggat actcgaggca ggaactcgaa cagatgcacc tctcagacct
    15241 ggacatcctg cctgcacgcg aggctgccgg gacatgggtc caggagatac gaaaaaaagg
    15301 catctccatc cgcgagacac tcctcaaaac ccgcgaagga gcgaccatac cggtcgagat
    15361 cagcagtaag gtaatcgagt actaccggtc cccggcggtc atcaccatca tcagagacct
    15421 ccgggcccgc aaggaggcgg agaatgcatt gcgggagagc gagaggcggt accggatgat
    15481 cggtgacctg atccccttcg gagtctggat caccgacgcc ggcgggacgc tcacctacgc
    15541 ttcgcaatcg ttcctggatc tcatcgggat gacgctcgat gagtgccgga ggaacggctg
    15601 gatgaaaaag ctcccggtcg aacagcgtga atccacccgg aatgactgga agcagtgcgt
    15661 cgagacaggc tgcttctggg actacgagta ccggatcgtc gatcggcagg gaaccgagca
    15721 cttcatcctg agcagggggg caccaatgag ggatgcctcg ggcaggatcg tctcgtgggt
    15781 tggcatccat ttcgatatct cgcagcgcag gcggtacgag aaccgcctgg aggcgtccct
    15841 ccgggagaag gaggtgatca taaaggaagt ccaccaccgg gtcaagaaca acatgcaggt
    15901 catctcgggg ttcctgcagc tccagaccaa ctacctcaca gaccaggagt caatcgagaa
    15961 actcgaggag tgccagatgc gggtaaagac gatggcgctc gtccacgaga agctttacca
    16021 gtccaagtat ctcgggttca tcaacacccg ggactatatc cagagcctca tctccgacct
    16081 gatgaactcg tacctgctca ggtccgacat ctcgatcgag caggacatcg acgatgtcaa
    16141 tatcaatctc gacaccgcca ttccctgcgg cctgatcctg aacgagctcc tcaccaatgc
    16201 cctcaagtat gcattcaatg gaagagaatc cggcaccata tcggtcagcc tgcacctcaa
    16261 ccccgaccac cgcttctccc tcgatatcag cgatgacggt gtcgggctcc cgccgggact
    16321 ggatattgcg caggcgccga ccctggggct gcagctggtg acagtccttg tccggcagat
    16381 aggcggcgag atgcaggtcg ggggtccccc ggggaccagt ttccacatca gtttccccga
    16441 gaagttctga caggggcatg acccggacac tctctcatgg ggccttccct cgtaggcttt
    16501 gcaccccgat cttcatgagt cccacccctg catatccttc accaggcctc tttggagggg
    16561 gagcctggaa agaaacaata ggttcaattt cgtccctgtc gtaacctttc atcacagatg
    16621 ggcagatacc atgcatatcc atgtcgaggg agtcgccggg ctccccatca tacataaggg
    16681 a
//
LOCUS       DAOQYR010000047        15945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_4106_length_15945_cov_8.982127, whole genome shotgun sequence.
ACCESSION   DAOQYR010000047 DAOQYR010000000
VERSION     DAOQYR010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 15945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15945
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4106_length_15945_cov_8.982127"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PKM87_06865"
     CDS             <1..248
                     /locus_tag="PKM87_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methanogenesis marker 12 protein"
                     /protein_id="HNQ29826.1"
                     /translation="RRIPALALFSAMECASLLLLAPGARVALAGTMAPVVAGEVRSLL
                     QTDVAVYDEWCAADGLALIAGEVFSGARDILGLEVHC"
     gene            380..922
                     /locus_tag="PKM87_06870"
     CDS             380..922
                     /locus_tag="PKM87_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HNQ29827.1"
                     /translation="MRELRIHGRGGQGSVTAAELIAVAAFAGGVYAQAFPAFGVERRG
                     APVQAFVRFDNKKVRLRSQVYEPDYIIVQDSTLIRDVNVFQGLKKDGIVIVNTEKALN
                     YPIPEGVKVITIDATSVALKHLGLPITNTTLMGAFAAASGEIALPALEGAIRERFRGE
                     LADKNVSAARDAYNLVKGVS"
     gene            926..1186
                     /locus_tag="PKM87_06875"
     CDS             926..1186
                     /locus_tag="PKM87_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ29828.1"
                     /translation="MALGLGCSVKPGKAKENKTGSWRTFRPEFQHEKCTKCGLCAQIC
                     PEGCIAEDEEGCPVPAYDYCKGCGLCAEECPSQAIEMKKEEK"
     gene            1188..2345
                     /locus_tag="PKM87_06880"
     CDS             1188..2345
                     /locus_tag="PKM87_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNQ29829.1"
                     /translation="MMEFMEGSHAVAHAVKYCRPEVIAAYPITPQTHIVEALADMVAN
                     CQLDAEYMTVESEFSALSACLGASAAGSRVYSATTSQGLALMFEVCFNCAGMRFPVVM
                     SIVNRAMGAPLSIWNDQQDSISLRDSGWLQFYAEDNQEAADLHYLAYKVAEDKNVLLP
                     AFVCFDGFILSHTYEPVDMLTQEQVDNYLPRYSPVQRLDSENPLSFGLFASPDYYMEF
                     RYQIDQAMKGAAKNIASAGSEFGEMFGRDYGDMIESYRLEDADCALVAMGSICGTVKD
                     AIDEMRKDGKKVGLMRLRTFRPFPGKEVRSALSNVSRVAILDKNISLGAKGAVALEVK
                     DALYGTQVPVLDYIIALGGRDVRKKDIRAVVEMAEKGKGDLFYGLRQELLA"
     gene            2342..3235
                     /locus_tag="PKM87_06885"
     CDS             2342..3235
                     /locus_tag="PKM87_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNQ29830.1"
                     /translation="MSGESCGLFEYGHRACGGCAAALAARTILDAAGKDTIVVSSTGC
                     MEVFSTPYPETAWKVPWIHSLFENNSAVASGIVASLKKQGRKEKVIALGGDGATFDIG
                     MLCISGAFERGHDFVYICYDNEAYMNTGIQRSGATPYDASTTTSPAGKCSSGNRRPKK
                     DLPMILAAHGAPYVATASVAYLPDLKKKVEKALATEGPCYLQVHAPCCTGWGFEGEKT
                     IAIGKLAIETGLWVNYEMEDGKVTRVKKVVRKPVETYLKEQRRFRHLFKPARNDAEIA
                     KVQAVADRNAERFGIDIKLKK"
     gene            complement(3345..3959)
                     /locus_tag="PKM87_06890"
     CDS             complement(3345..3959)
                     /locus_tag="PKM87_06890"
                     /inference="COORDINATES: protein motif:HMM:NF039237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5803 family protein"
                     /protein_id="HNQ29831.1"
                     /translation="MRASHGDRCRFALVALCLTALLTILPAAALGAEYRITQNGTSYR
                     AALDLVDAERFEFHEIGMLGERIPLQVSDVALSGDCNPCEFEQSGNAAITFQRGNYTL
                     FFQGPVRDNHFVATFESPRRVSVLLPPGLDVRNPALGMISQGGIVTDSGVEGIIVTWN
                     STRTAEVRFYDEGRENLLYIFANFWIIIAVVLLVPFLLTWKRRE"
     gene            complement(3893..5101)
                     /gene="thrC"
                     /locus_tag="PKM87_06895"
     CDS             complement(3893..5101)
                     /gene="thrC"
                     /locus_tag="PKM87_06895"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNQ29832.1"
                     /translation="MYRLVCVECGESYPPAEILYHCTRCGHLLAVEYDLESAHVDRAA
                     WDHRPLSVWRYREILPVSIPPVSLHEGGTPLYHLSRIGEEIGLPHLYAKHEGMNPTGS
                     FKDRGMTVGVSMALQLGKTSVACASTGNTSASLAAYAAKAGIPAVVLLPAGKVALGKV
                     AQALMHGARVLSIRGNFDRALEMVHELCLSHGLYLLNSVNPFRLEGQKTIGFEALDQL
                     GEIPDRMVLPVGNAGNISAVYKGFTELASLGLMDRIPMMTGIQASGSSPVVRAIHEGL
                     PELIPEANPETVATAIRIGAPVNAEKALTAIRKTGGTAESVTDEEILAMQRELARKEG
                     IGVEPASAASVAGVKKLREMGVIDRDEKIVCVVTGHLLKDPETVIRQCEPPMEIDADL
                     PSLLSALQRS"
     gene            complement(5235..6041)
                     /locus_tag="PKM87_06900"
     CDS             complement(5235..6041)
                     /locus_tag="PKM87_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNQ29833.1"
                     /translation="MKLFCAQVASVWEDPQATLNKVEPCIARASRGGGDLICFPEQFA
                     TGWDPGSSQHVQGIDGAITGRLCSLAQEYSIAILGSFREKTKGNPRNTSVVISSEGEI
                     ISTYAKCHLFSPASEDRHYQQGNTIATFPVGGIECGLAICYDLRFTSLFRLYADAGVH
                     AVLVPAAWPEARLQHWELFIRARALEYQVYVAGINTTGINPIDIYAGCSMVADPTGGV
                     IVRAGPGEALISCELDREYVERVRSALPVTRDRRDDLSPAQETKRQGKAG"
     gene            complement(6675..7124)
                     /gene="ndk"
                     /locus_tag="PKM87_06905"
     CDS             complement(6675..7124)
                     /gene="ndk"
                     /locus_tag="PKM87_06905"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNQ29834.1"
                     /translation="MDRTFVMVKPDGVARGLIGEVVSRLEQKGFKLVAARLEKLPEKR
                     VMEQYREHLEKPFFPSLRHYIESGPCFLMVWEGKDAAAVVRRVVGATNPAEAAPGTIR
                     GDLAMDIGRNVIHASDSPGSAAREIAIHFPATEIVSYKRVDEAVLYE"
     gene            complement(7133..7324)
                     /locus_tag="PKM87_06910"
     CDS             complement(7133..7324)
                     /locus_tag="PKM87_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24e"
                     /protein_id="HNQ29835.1"
                     /translation="MVEQRNCSFCGDAIEPGTGKMFVRRDGTVYYFCSTKCQSNFKLG
                     RAARRVPWTQAGRKAMGKE"
     gene            complement(7332..7541)
                     /locus_tag="PKM87_06915"
     CDS             complement(7332..7541)
                     /locus_tag="PKM87_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S28e"
                     /protein_id="HNQ29836.1"
                     /translation="MADEGTPAEVIEVIGSTGMHGEAMQVKCRILDGNNKGRIITRNT
                     VGPIREGDVLMLLETEREAKKLSRR"
     gene            complement(7549..7917)
                     /gene="rpl7ae"
                     /locus_tag="PKM87_06920"
     CDS             complement(7549..7917)
                     /gene="rpl7ae"
                     /locus_tag="PKM87_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7Ae"
                     /protein_id="HNQ29837.1"
                     /translation="MAKTYVKFQVSEEIQNKALEALEVARDTGKIKKGSNEATKAIER
                     SIASLVLIGADVEPEEIVMHLPLLCEEKKIPFIFINKQNDIGTASGLDVGSAAAAIVK
                     PGKAKEMIDEIAKQVADLRA"
     gene            complement(8056..10107)
                     /locus_tag="PKM87_06925"
     CDS             complement(8056..10107)
                     /locus_tag="PKM87_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNQ29838.1"
                     /translation="MKGAFREFAYPVSFPASLLVFSLLSWYCGLLRLPILLALAPFAI
                     LFLRAAWKGEYSKKAFEGQGVWVFLFLLFFFAMVSVRFVNPSISYAEKFMDHAFLASI
                     MRVPVVPPPDPWFLGGYLNVYYYFGYWMIGAVGIVSGVPSYIAFNLALPTVLGLSAMM
                     MYALGRLLTRRFAYLTLATFLVANPSFIWQIIQGKSLGTVFWDSTRTIPDTINEYPLF
                     SFLWGDVHPHVIGIFNQVLFLFILVYAWTRWKSLQPQDRMGVILLAALSLGAMPLINT
                     WDVIVYAPVTVLIGLVIWYQGGRPSPAPGAGNLEILYSSGRRVISGLARFLEEKTWQA
                     ERTLVPTLERIASAPYAFLLLVPVVSIVLYLPFYFQMNTRGIQGVGLVHTPTGPVDFL
                     LVHGLFLVLMVIYLYRDIWKHPLLLIIPFIIWLSGHGAAALAAIPLVYLLCRRTRNPA
                     EVLAILGLSIAIMCEVFYLVDNMGETYYRMNTIFKFYISAWLLMGASSFAMLAEMLDR
                     RMGEIPSPALLARAALIAVALFLLVTPLVLPLDSPYRGATLDGLDYVNSAYPGDAEAV
                     AFLRTLPGVNGIVEAVGGDYTYYSRVSSFTGIPAVMGWSFHEFMWRDDTDGWYGRRID
                     DVKTMYEKPDQTESLMRSYNATHIYVGDLEREQYGVRVHEAGLLLVYDRAGVQIYALP
                     G"
     gene            complement(10178..11149)
                     /locus_tag="PKM87_06930"
     CDS             complement(10178..11149)
                     /locus_tag="PKM87_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNQ29839.1"
                     /translation="MWRKISTIFISTAIAVGIIAFMLFRIWDDLLVALEHAVWYYLLL
                     ASLVCLAAWWVRGLRYRSILASLGIPVGVNFSTACIFVSQTANLIVPARLGDLVRIFI
                     LGHEKNAPVSKGISSLVVERVFDIVTVALLGAISLLFVLNVPDWFYTLILLPLAAGAV
                     FFAVLLFIGKFNSENRIIKTLLTIMEQIKEASLSVRAVVILSGSSILIWLMDVMVCVF
                     VVMMFQEEIPFAAVVLAIVIGNLVKAVPLTPGGVGTYELALALTFTLVGVPAAEAAMI
                     AVIDHLVKNLITLVGGIASIYYFGGWVLDSIKSAFEKRFGGIKPGGV"
     gene            complement(11133..11849)
                     /locus_tag="PKM87_06935"
     CDS             complement(11133..11849)
                     /locus_tag="PKM87_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ29840.1"
                     /translation="METPEVSAVIPVYNDRDSLREAIPRSIEALAAIDPGFELIVAED
                     GSADGSANEVMVWEQRDARVRLLHSDARLGRGRALTRAFAEARGRIVCYYDVDLATDI
                     SHLPELIGAIRDGADAATGSRLLPESTIQRSGGRELASRGYNLMVRTILGSRLYDHQC
                     GFKAFRKDRLMDLLPSVRDTHWFWDTEVLIRAQRKGYRVVEFPVRWREGSGTTVKKND
                     VYRMGRSILGLWWQLHVEKD"
     gene            complement(11851..13077)
                     /locus_tag="PKM87_06940"
     CDS             complement(11851..13077)
                     /locus_tag="PKM87_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ29841.1"
                     /translation="MKICIIGGGLTGLTAAYCLGQDHEVDLYEKRLSLGGCVSSYEIG
                     DYSIEEFYHHCFAGDSHLFSLMESLHVADRLEWLKGTTGYYADSTIYPLNSPTEILRY
                     PLLSFSDKIRLTFLTLRSRKIDTGPLDGITARDFVTREIGERAYQSFFEPLLASKFGD
                     RKDEISAAWLISRIAIRSNRGLSGEKLGYLKGGFFTLIDALSEQVGKHCVIRLQDPVT
                     SLSHETHEWRVNGQRYDAVISTIPPHTLPGGTLSHLPTIPYQGAACMLLSLDHDVTSG
                     IYWLNMKDPAPYGAVVAHTNFIPLERYGEHLVYLASYFHGTLPPRKDQAMLEDFCGRF
                     HVQPGDIRWHRMAVDQFAGPLYTTGFRKRVLPYQEKGLYLAGMFSGPNYPERSMEGAV
                     CAGEEVAALLARQEGA"
     gene            13260..14438
                     /locus_tag="PKM87_06945"
     CDS             13260..14438
                     /locus_tag="PKM87_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin/FtsZ family protein"
                     /protein_id="HNQ29842.1"
                     /translation="MRIFFIGFGQAGGKIVDMFLEQDRKLGRNSFRGIAVNTARTDLM
                     GLKHIDLKDRILIGQTVVKGHGVGTDNATGAKITFDEIDSIISAIDKRGTHDVDAFMI
                     VAGLGGGTGSGGTPVLTRSLKKVYREPVYVLGILPAPEEGRLYSYNAARSLATLVKEA
                     DNTFIFDNSAWKNEGESIKSAFSRLNDEIVRRFGVLFRAGEVGSAGIGEMVVDSSEII
                     NTLRGGGITSVGYAITEVITKKQKKSVGGILYGIKGSLRKKEAIEEVLLGEDRTAKVI
                     SLVRRAMLGRLTLPCDFSSTERALVLVAGPPDELDRKGIEKAKSWVEENIAGVEVRGG
                     DYPVNSNFVAAVVVLATVGNAPRIKELMEIARSTRDDAAKIEEKKPLSFEEEIEPLFE
                     "
     gene            14496..15584
                     /locus_tag="PKM87_06950"
     CDS             14496..15584
                     /locus_tag="PKM87_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29843.1"
                     /translation="MQGVVTAIAVGNIAITPSTDLVSGQTQVRSTFVINFPASGGYTF
                     DDANSLQLASEMDTPSWTYAIVMDGIENPSKTEIGQNININGWLLSYPSKRELSMKVT
                     MEGVAPTVTGSEEKIVIAVRELDSRGAKITSSEVVKKKMVINPAQIQVSINQAKESLA
                     AMRGQIDQLAASGIDTSPIEQKYSEASTAIHTAEGASDYTRAQQSLNTANAALADAGT
                     MVREFGIQKSINDVDAVIGQVNTLITDFRVNRSMSSDARLTPIILAYDNAANMVSAAK
                     DELSRKEYDQATSKANDARVKADEALAEALKLKEQLDANPLAAAGTMFAGVLTGGIVI
                     IIVVAVIAVIAVLGLLFFRRRRKWDELG"
BASE COUNT         3455 a         4479 c         4862 g         3149 t
ORIGIN      
        1 aaaggagaat tcccgcactc gcgctttttt ctgcgatgga atgcgcctcc ctcctcctcc
       61 tcgcgcctgg agcccgcgtt gcccttgcgg ggaccatggc cccagtggtg gcgggcgagg
      121 tgagatctct cctccaaaca gatgttgcag tctacgacga atggtgtgct gcggatggtc
      181 ttgccctgat tgcaggagag gtcttctcgg gtgccaggga catcctgggc ctcgaagtgc
      241 actgctgatt gcaggtttcc ttgcaggacg aactcggaca cggaacggct tgcgtctgtc
      301 agggtactcc agtccctccg cagcccaaaa tctcaacatt ttagtatgat taatcatgta
      361 aaattatact gtgatttctt tgcgcgagct gcgtattcat ggccgtggcg gacagggttc
      421 ggtcaccgcc gcagaactga tagccgtggc ggcattcgcg ggcggggtgt atgcccaggc
      481 gtttccagcc ttcggggtgg agcgcagggg tgccccggtc caggcgttcg tccggttcga
      541 caacaagaag gtcaggttga gaagccaggt gtacgagcct gattacatca tcgtgcagga
      601 cagcaccctg atccgggacg tgaacgtatt ccagggactc aagaaagacg ggatcgtgat
      661 cgtgaacacc gagaaggctc tcaattaccc gatccccgag ggcgtgaagg tcattaccat
      721 tgatgccact tctgttgcgc tgaagcacct gggcctcccc atcaccaata ccaccctgat
      781 gggggcgttt gccgctgcca gcggtgagat cgcgctcccg gcgctcgagg gagctatccg
      841 ggagcgcttc cggggcgaac tggcagacaa gaacgtctcc gcagcccgcg atgcgtataa
      901 ccttgtgaaa ggggtgtcct gaaccatggc actcggtctt ggctgctcgg taaagccggg
      961 aaaagcaaag gagaacaaaa caggctcgtg gaggacattc cgccccgagt tccagcacga
     1021 gaagtgcacg aagtgcggtc tctgcgccca gatatgcccc gaagggtgta ttgctgagga
     1081 cgaagagggg tgccctgttc ccgcatacga ttactgcaag gggtgcggcc tctgcgcaga
     1141 ggagtgcccg agccaggcaa tcgagatgaa aaaggaggag aaataagatg atggagttca
     1201 tggaagggtc gcatgccgtc gcccacgcgg tgaagtactg ccgcccggag gtcattgcag
     1261 cctatcccat cactccccag acccacatcg tggaggcgct ggcggacatg gtggcaaact
     1321 gccagctgga tgcagagtat atgaccgtgg agagcgagtt ctccgccctc tccgcatgcc
     1381 tcggcgcaag cgctgccggc tcaagggtct actccgccac cacctcccag ggcctcgccc
     1441 tgatgttcga ggtctgcttc aattgtgcag ggatgcgctt cccggtcgtg atgtccatcg
     1501 tgaacagggc gatgggagcc ccgctctcga tctggaacga ccagcaggac tccatctcgc
     1561 tccgggactc gggatggctc cagttctatg cagaggacaa ccaggaggcg gctgacctcc
     1621 actaccttgc atacaaagtg gccgaggata aaaacgtcct tctccccgcc tttgtctgtt
     1681 tcgacggatt catcctctcc catacctacg agccggtcga catgctcacc caggaacagg
     1741 ttgacaacta cctcccacgg tattccccgg tccagcgcct ggacagcgag aaccccctct
     1801 cctttggcct ctttgcatcc cctgattact acatggagtt ccggtaccag atcgaccagg
     1861 cgatgaaggg agccgccaaa aatattgcga gcgcgggatc ggaatttgga gagatgttcg
     1921 ggagggatta cggcgacatg atcgagtcct atcgcctcga agatgcggac tgtgcactcg
     1981 tggccatggg ctcgatctgc gggactgtca aggacgcgat cgacgagatg agaaaggatg
     2041 gaaagaaggt tggtctcatg aggctgcgca ctttccgccc gttccccggg aaggaggtga
     2101 gatcggcact ctcgaacgtc agcagggtgg cgatactcga caagaacatc tctcttggtg
     2161 caaagggcgc agtggcgctc gaggtgaagg atgcgctgta tgggacgcag gtcccggtcc
     2221 tcgattacat cattgccctc ggaggaaggg acgtgagaaa gaaggacatc cgggccgtag
     2281 tggaaatggc cgaaaaaggg aagggagacc tgttctacgg tctccgtcag gagttgttgg
     2341 catgagcggg gaatcgtgcg gcctgtttga gtacgggcac agggcatgcg ggggctgcgc
     2401 ggcagcactc gcagcccgga ccatcctgga tgcggcagga aaggatacga tcgtggtatc
     2461 ctcgacgggg tgtatggagg tcttctccac tccctatccc gagaccgcat ggaaagtccc
     2521 ctggatccat tcgctctttg agaacaactc ggcggttgct tccgggatcg tggcgtcgct
     2581 gaagaaacag ggccggaaag agaaggtgat cgcgctcggc ggcgacgggg cgacgtttga
     2641 tatcggcatg ctctgcatat cgggtgcgtt cgaacggggt cacgacttcg tctatatctg
     2701 ctacgataac gaggcctaca tgaacaccgg tatccagcgg tcgggtgcaa ccccgtacga
     2761 tgcgagcacc accacgagcc cggcagggaa gtgctccagc gggaaccgcc gcccgaagaa
     2821 ggatctcccc atgatcctcg ctgcacacgg ggcgccctac gtggccaccg cgtctgtcgc
     2881 gtacctcccc gacctgaaga agaaagtcga aaaagcgctt gcgaccgagg gtccctgcta
     2941 cctgcaggtg catgctccct gctgcacggg gtggggattc gagggggaga agaccattgc
     3001 gataggaaaa ctcgcaatcg agacggggct ctgggtcaat tacgagatgg aggacggcaa
     3061 ggtgacaagg gtgaagaaag tggtgagaaa gcccgtggag acctacctga aggaacagcg
     3121 ccgcttccgc cacctcttca agccagccag gaacgatgcc gagatcgcga aggtccaggc
     3181 ggttgccgac cggaacgcgg agcggttcgg gatcgatatc aagctgaaga aataacacaa
     3241 ctttttttgt atccccaaac cccgtgtata tttcctgcgt atgccttccc ggacaggcaa
     3301 ctgccccgga atatcccttc ttccttgacg gattaccggg gaccctactc tctcctcttc
     3361 caggtgagga ggaacgggac gagcaatacc acagcgatga tgatccagaa attcgcaaag
     3421 atgtagagca ggttctccct gccttcgtca tagaaccgga cttcggcagt ccttgtggaa
     3481 ttccaggtga caatgatgcc ctccacgcca gaatcagtca cgatacctcc ctggctgatc
     3541 atgccgaggg cgggattcct tacatcgagc ccgggaggga gtaagacgct gacccgcctg
     3601 ggagactcaa acgtggcgac aaagtggttg tcgcggacag gcccctggaa aaagagggta
     3661 taattgcccc gctggaatgt gatggcggcg tttccgctct gctcaaattc gcaggggttg
     3721 cagtcgccgg agagcgcaac atccgacacc tgcaggggga tgcgctcgcc aagcatcccg
     3781 atctcgtgga actcgaaacg ctctgcgtcc acgagatcaa gcgctgcgcg atacgaagtc
     3841 ccgttctggg tgatgcggta ctctgcacca agcgcggctg ccggcaggat cgtcaggagc
     3901 gctgtaaggc agagagcaac gagggcaaat ctgcatcgat ctccatggga ggctcgcatt
     3961 gcctgatcac cgtctccggg tctttcagca ggtgcccggt caccacgcat acaatcttct
     4021 cgtcacggtc gatcacaccc atttcacgga gtttctttac ccctgcaacg gaggccgccg
     4081 aggcgggctc gaccccgatc ccttcttttc gtgcgagttc ccgctgcatg gcaaggatct
     4141 cttcgtcggt cacggactcg gcggtcccgc cggtcttccg gattgcggtc agtgccttct
     4201 cggcattcac cggtgccccg attcggatag cggttgccac ggtctcggga tttgcctcgg
     4261 gaatcaattc gggcagtccc tcgtgtattg ccctgacgac ggggctcgat ccggatgcct
     4321 ggatcccggt catcatcggg atgcggtcca tcagcccgag tgatgcgagt tcggtgaatc
     4381 ccttgtagac ggcggagatg ttgcctgcgt ttccgaccgg gagcaccatc cggtccggga
     4441 tctccccgag ctggtcgagt gcctcaaacc cgatggtctt ctgcccctcg aggcggaagg
     4501 ggttgacgga gttgaggaga tagaggccgt gggagaggca gagttcgtgc accatctcaa
     4561 gcgcccggtc gaagtttccg cggatggaga gtacccgtgc accgtgcata agggcctgtg
     4621 cgacttttcc gagcgccact tttccggccg ggagcagcac gactgccggt atgccggcct
     4681 ttgcggcata ggcggcgagg cttgccgaag tattgccggt actcgcgcag gcgacggagg
     4741 tcttgccaag ctgcagcgcc atgctcacgc cgaccgtcat gccgcggtcc ttgaacgaac
     4801 cggtgggatt catgccctcg tgcttggcat agaggtgtgg aagccctatc tcctccccga
     4861 tacgtgaaag atggtagaga ggagttcccc cttcgtggag cgagacaggg ggaatggaga
     4921 ccgggaggat ctcccggtac cgccataccg agagcggtct atggtcccag gcagcccggt
     4981 ccacgtgtgc ggattcgagg tcatactcca ccgcgaggag gtggccgcag cgcgtgcagt
     5041 gatataatat ctcggcaggc gggtatgatt ccccgcactc tacgcacacg agacggtaca
     5101 tgaatacatg tgtgcatccg gaggggaaaa aggaggtgca cgcggcggaa acggtgcccg
     5161 gaacctgctg aaaagtcgcg aacgggagaa aagaaatctc ctttcgtttt ccggcaatgg
     5221 atcgactgcc ccggtcagcc tgccttcccc tgtcgtttcg tctcctgtgc cggtgagagg
     5281 tcatcccgcc tgtccctggt gacggggagc gcggatctca cccgctccac gtattcccgg
     5341 tcgagttcgc aggatatgag tgcctctccc gggcctgcac ggacgatgac gccgccggtc
     5401 gggtcggcca ccatggagca gccggcatat atatcgattg ggttgatgcc ggtcgtgttg
     5461 atcccggcta catagacctg gtattcgagt gcacgggcgc ggatgaagag ttcccagtgc
     5521 tggagccgtg cttccggcca ggccgcaggt accagcaccg cgtggacgcc agcgtctgcg
     5581 tacagccgga agagtgaggt gaaacgaagg tcgtagcaga tggcgagccc gcactcgatc
     5641 ccgccgacag ggaacgtggc gatggtattc ccctgttggt agtgccggtc ctctgacgct
     5701 ggggagaaga ggtggcactt ggcgtaggtt gaaataatct ctccctcgga agagattacg
     5761 acggacgtgt tccgcggatt tcccttggtt ttctcgcgga aagagccaag gatcgcgatg
     5821 gaatactcct gcgcgagcga acacagtctt cccgtgatgg ccccgtcgat cccctgcacg
     5881 tgctgggacg acccggggtc ccacccggtg gcgaactgct cggggaagca gatgagatcg
     5941 ccaccaccgc gcgatgcccg ggcgatgcag ggctccactt tattcagggt ggcctgcggg
     6001 tcctcccaga cacttgccac ctgggcgcag aagagcttca taccagtggt gtggggggcg
     6061 agaatacttt gatatttcct gccggagcat gagggtgtcc aaaaggagcc cctcgacaag
     6121 ggggcgacct ccagatcacc atgaatcacg gtttaactca acaggatagg aaatctcgca
     6181 ggtaacttgg ccattaatgt tttcatcagc ggggaggggg agaatccccc tccccagtcc
     6241 cctccctcta atatgggata gcctgtgggc agggttttgc agggaaatga tgtgatgccc
     6301 caactgctcc cccgcactgg cctcatcgca atcaggggtt gccacagcgg cggggcgaag
     6361 ccccgagagc gtcatcttga ggggagtcgc ggtttttcat agcgagagaa tttcaagtgt
     6421 attagaaact taaatgaggg ggagaatccc catcccccac cccctccccc taatacgcgg
     6481 taacctgtgg tcagggttgc atggggacgc agatgaaaca ggccttgcgc ccaggcacta
     6541 atcgctccca aatgagagcg tggctccgtc gtcctaaata gtggcaggaa cgcaaatttt
     6601 gcaggagaga aaatttacac tcactcaagg gaataaaaaa cccttgaact ggtgattggg
     6661 aaaaaaagaa tgagttactc gtagagcacc gcttcatcca cccgcttgta ggagacgatc
     6721 tccgttgcgg ggaagtggat ggcgatctcg cgggcggcgc tcccggggga atcggacgcg
     6781 tggatcacgt tcctcccgat gtccattgcg aggtcgcccc ggatggtccc cggcgcagcc
     6841 tctgccgggt tggtggcgcc gacgacccgc ctgaccacag cggcagcatc cttgccttcc
     6901 cagaccataa ggaagcaggg gccgctctcg atgtagtggc gaagcgaagg gaagaacggc
     6961 ttctccaggt gttccctgta ctgctccatg accctcttct cgggcagctt ctcaaggcgt
     7021 gccgccacca gcttgaagcc cttttgctcc aggcgggaga cgacttcgcc gatgagccct
     7081 ctcgcaacgc cgtcaggctt gaccatcacg aaggtgcggt ccatggagtg gctcactcct
     7141 tgcccatcgc ctttctgccg gcctgggtcc agggcacccg ccgggctgcc cggccaagct
     7201 tgaagttgct ctggcacttg gtgctgcaga agtagtagac cgtgccgtcc ctccggacga
     7261 acatctttcc ggtccctggc tcgattgcgt ccccgcagaa gctgcagttt ctctgttcga
     7321 ccatgatctc ctcaccgcct ggagagcttc ttggcctcgc gttcggtctc cagcagcatc
     7381 agcacatccc cctcgcggat gggcccgacc gtgttgcggg tgatgatccg tcccttgttg
     7441 ttgccgtcga ggatcctgca cttcacctgc atggcctcgc cgtgcatccc ggtggagccg
     7501 atcacttcga tcacctctgc gggagttcct tcatctgcca tggcccgctc acgctctcag
     7561 atcggcgacc tgcttggcga tctcgtcgat catttccttc gctttccctg gcttgacaat
     7621 ggcggcggca gcagatccga cgtccagccc gctggcggtg ccgatatcgt tctgcttgtt
     7681 gatgaagatg aatgggatct tcttctcctc gcagagcagc ggaaggtgca taacgatctc
     7741 ttccggctcg acatcggctc cgatcagtac aagcgaggcg atactgcgct cgattgcctt
     7801 cgtcgcctcg tttgacccct tcttgatctt ccccgtgtcc ctggcaacct ccagggcttc
     7861 gagggccttg ttctgaatct cttcagaaac ctggaatttc acatatgtct tggccataac
     7921 tcacctcatt caggagtagc gtagtctccc tcatcactca tcagtcagtg acgacgatgt
     7981 agccatatca tttcgacaga tgcagacata aaggtttgga agaacgcggt tttccactgg
     8041 gtttcaggcc gggaatcagc cgggaagggc gtaaatctgc actccggcgc ggtcgtacac
     8101 gagcaaaagc cccgcctcgt ggacccggac tccatactgc tcccgttcaa ggtctcccac
     8161 gtagatgtga gttgcattgt aggaccgcat cagcgactct gtctggtcag gcttctcgta
     8221 catggtcttc acgtcatcga tccgtctccc ataccatccg tcggtgtcgt cccgccacat
     8281 gaactcgtgg aacgaccagc ccatcacggc cggaatccct gtaaaggaag agacccgtga
     8341 atagtaggtg taatcccccc ccaccgcctc gacgatgcca ttgacgccgg gaagagtccg
     8401 gaggaacgcc accgcctccg cgtctcccgg ataagccgag ttcacgtagt caaggccgtc
     8461 cagcgtcgcg ccacggtagg gggaatcgag aggaaggaca aggggtgtga ccagcaggaa
     8521 caatgctacg gcgatcagcg cggccctggc gagcaatgca ggggatggga tttcgcccat
     8581 ccgccggtcc agcatttctg cgagcattgc aaacgacgag gcacccatca gcagccaggc
     8641 agagatgtag aacttgaaga tggtgttcat ccggtagtag gtctctccca tgttgtccac
     8701 caggtagaac acctcgcaca tgatggcgat ggagagcccg agtatggcga ggacttccgc
     8761 ggggtttctc gtccgcctgc aaagaagata gaccagcggg atggccgcaa gcgcggctgc
     8821 gccgtgtcct gaaagccaga ttatgaaagg gatgataagg agaagggggt gtttccagat
     8881 atccctgtac aggtagatga ccatgaggac caggaacagg ccgtgcacaa gcaggaagtc
     8941 cacggggcct gtcggtgtgt gcacgaggcc cacgccctgg attccccggg tgttcatctg
     9001 gaagtaaaac gggaggtaga ggacaattga gacgaccggg accaggagaa ggaatgcata
     9061 cggggcagat gcaatcctct caagtgtggg gacaagcgtt ctctctgcct gccaggtctt
     9121 ttcttccagg aaccgagcaa ggcccgaaat gactcttctt cctgacgaat acaggatctc
     9181 caggttcccg gcaccgggcg caggagatgg cctgcccccc tggtaccata tcacgaggcc
     9241 gatgaggaca gtcacagggg catagacgat gacgtcccag gtgttgatga gcggcatggc
     9301 gccgaggctg agcgccgcaa ggagaatgac tcccattcgg tcctgcggct ggagcgattt
     9361 ccagcgggtc caggcataga cgaggatgaa gaggaagagc acctggttga agatcccgat
     9421 cacgtgggga tggacgtccc cccacaggaa tgagaagagg ggatactcgt tgatggtgtc
     9481 ggggatggtc cgtgtgctgt cccagaagac ggtcccaagg ctctttccct gtatgatttg
     9541 ccagataaaa gatgggtttg caaccaggaa tgtggcaagg gtaaggtacg cgaaccggcg
     9601 cgtcaggaga cgccccagcg catacatcat catggccgag agcccgagca cggttgggag
     9661 tgccaggttg aatgcgatat acgaaggcac cccgctcacg atcccgaccg caccgatcat
     9721 ccagtatccg aagtaataat agacgttcag gtatcccccg aggaaccagg ggtccggcgg
     9781 tggaacgacc ggcacccgca tgatggaggc gaggaacgcg tggtccatga acttctcggc
     9841 ataagagatg ctcgggttga cgaacctgac actcaccatc gcaaagaaga agagcaggaa
     9901 gagaaacacc cataccccct ggccttcgaa ggctttcttg gaatattcac ccttccacgc
     9961 ggcccgcaga aagagaatcg cgaagggggc gagggcgagg agtatcggga ggcggaggag
    10021 cccgcaatac caggagagca gggagaagac aaggagggat gccgggaacg agaccgggta
    10081 ggcgaactcg cggaatgccc ccttcaggta cggccagaga gccagctgga ggaagaagag
    10141 caggagcagc caggagatga ctgccagggc ctggaactca taccccgcca ggttttatcc
    10201 ctccgaatcg cttctcgaag gcgctcttta tgctgtcaag gacccacccg ccaaaataat
    10261 agatagaggc gatacccccg acgagcgtga taaggttctt gaccaggtga tcgatcactg
    10321 cgatcatcgc ggcctctgca gccgggactc ccacaagggt gaacgtaagc gcaagggcca
    10381 gttcgtatgt cccaacccca ccaggagtga ggggaactgc cttcacgagg tttccaatga
    10441 cgatggcgag gaccactgcc gcgaatggga tctcctcctg gaacatcatc accacgaaga
    10501 cacagaccat cacgtccatc agccatatga gaatggaact gcctgagagg atgactactg
    10561 cccggacgga tagagaggct tcttttatct gctccatgat ggtgagcagg gtcttgatga
    10621 tcctgttctc agagttgaac ttgccgataa agagcagtac tgcgaagaag accgccccgg
    10681 cagcgagcgg gagcaggatg agggtataga accagtcggg gacattcagg acaaaaagga
    10741 gagatatggc cccgagaagg gcgaccgtga cgatgtcgaa cacccgctcc acgacgaggg
    10801 acgagatccc ctttgagacc ggggcgttct tctcatgccc gaggatgaag atcctgacaa
    10861 ggtcgccgag ccttgcgggc acgatcaggt ttgcggtctg tgatacgaag atgcatgccg
    10921 tggagaagtt cactccgaca gggatcccga gcgaagcgag gatgctccgg tatcgaagac
    10981 ccctcaccca ccaggcggcc aggcagacga gcgaggcaag gaggaggtaa taccacacgg
    11041 cgtgctccag ggcgacaagg aggtcatccc agatgcggaa gagcatgaat gcgatgatcc
    11101 ccacggcgat cgcggtagaa atgaagatgg tactaatctt tctccacatg caactgccac
    11161 cacaacccaa gaattgacct tcccatccgg tagacgtcgt tctttttcac tgtcgtcccg
    11221 gatccctctc tccaccggac cgggaactcg acgacccggt aacccttccg ctgtgcccgt
    11281 atcagcacct cggtgtccca gaaccagtgg gtgtcccgaa cagacgggag gagatccatg
    11341 aggcggtcct ttcggaacgc cttgaacccg cactggtggt catagagcct gcttccgaga
    11401 attgtgcgca ccatgaggtt gtagccgcgg ctcgcaagct cgcggccccc gctcctctgg
    11461 attgtgctct cgggcaggag ccgtgagcct gtagcagcgt cggccccatc ccgtatggcc
    11521 ccgatcaact cggggaggtg ggagatgtct gtggccaaat ccacgtcgta atagcagaca
    11581 atacgtcccc gggcctcggc aaatgccctg gtgagggccc ggccccggcc cagccgggca
    11641 tcgctgtgca ggagccttac gcgggcgtcc cgctgttccc ataccatgac ctcattcgcg
    11701 ctcccgtcag cgctcccatc ctcggcgacg atgagttcga aacccgggtc gatggctgcc
    11761 agcgcctcga tcgacctggg aattgcctcc cggaggctgt ccctgtcatt gtacacggga
    11821 atgacggcag agacttccgg ggtctccatg tcaggcccct tcctgccggg cgagcagtgc
    11881 agcaacctct tccccggcac aaacggcccc ctccatgctc cgttcgggat agtttgggcc
    11941 ggagaacatc ccggccaggt agaggccttt ctcctggtag ggcaggaccc gcttgcggaa
    12001 cccggtggta tagagaggcc ccgcgaattg atcgactgcc atccggtgcc accggatgtc
    12061 ccccggctgg acatggaacc tgccgcagaa gtcttcgagc atcgcctggt ctttcctggg
    12121 agggagggtg ccatggaagt aggacgccag gtacacgagg tgctccccgt accgctcaag
    12181 gggaatgaag ttggtgtgtg cgacgactgc tccgtagggg gccggatctt tcatgttcag
    12241 ccagtagatg ccggacgtga cgtcatggtc caatgagagg agcatgcacg cggccccctg
    12301 gtaagggatc gtggggaggt gggaaagcgt tcccccgggg agggtatgag gggggattgt
    12361 cgagatcact gcatcgtatc tctggccgtt caccctccac tcatgggtct catgggagag
    12421 cgacgttacc ggatcctgga gccgtatcac gcaatgcttc ccgacctgct cggagagtgc
    12481 atcgatcagc gtaaagaagc cccctttcag gtatccgagc ttctctcctg aaagaccacg
    12541 gttcgagcgg atggcaatcc ggctgatgag ccatgctgct gaaatctcgt ctttccggtc
    12601 cccgaatttg ctggcgagca ggggttcgaa gaatgactgg taggcacgtt ctccaatctc
    12661 cctcgtgaca aagtcgcgtg ctgtgatccc atccagaggc cctgtgtcga tcttccgcga
    12721 ccgcagcgta aggaatgtca gccgtatctt gtcggaaaaa gagaggagcg gataacggag
    12781 tatctctgtt ggcgaattga gtggatagat ggtactgtcc gcgtaatacc cggtggtccc
    12841 cttcagccac tccagccggt ctgcaacatg gagcgattcc atgagagaga agaggtgtga
    12901 gtctcccgca aagcagtgat ggtagaactc ctcgatggaa tagtccccga tctcgtagga
    12961 tgagacgcac cctccaagtg agaggcgttt ctcatagaga tcgacctcgt gatcctgtcc
    13021 gaggcagtag gccgctgtaa ggcctgtcag gcctccgcct atgatgcaga tcttcatgtc
    13081 cggcgctcag tataggttgg aaaaagggga tgaaaaaggc tccggattcc aaatatcgca
    13141 attaaattca gtttgcatca aatggaaaag aaggaaagta tttgaactcc cggaagaaac
    13201 catattcatt gagtcaattt gagcatcttg cgtaatttgt gggctggtgg gtcgttttca
    13261 tgagaatatt cttcataggt ttcggtcagg cgggcgggaa gatcgtggat atgttcctcg
    13321 agcaggacag gaagctggga cgaaacagtt tccggggcat tgccgtcaat accgcccgca
    13381 ccgacctcat gggtctgaag catatcgatt tgaaagaccg tatcctcatc gggcagactg
    13441 tggtcaaggg acatggcgtg gggacggaca atgcaaccgg ggccaagatc acattcgacg
    13501 agatcgacag catcatcagc gccattgaca agcggggcac ccacgacgtc gatgcattca
    13561 tgattgttgc agggcttgga ggaggcacag ggtctggggg cacaccggtg ctgaccaggt
    13621 cgctgaagaa ggtctaccgg gaaccagtgt acgtcctcgg tatccttcct gcgcccgagg
    13681 aaggcaggct ttattcgtac aacgctgcaa ggagcctggc gacactggtc aaagaggcag
    13741 acaacacgtt catctttgac aacagcgcct ggaagaacga aggggagagc ataaagtcag
    13801 cattttcccg cctgaatgac gagatagtcc gcaggttcgg agtactcttt cgtgcaggag
    13861 aagtgggatc ggccggtatc ggggagatgg ttgtcgactc aagcgagatc atcaacactc
    13921 tccgtggcgg ggggatcacc tctgtcggat atgccatcac cgaggttatt acgaagaagc
    13981 agaagaaatc cgtcgggggt attctctatg gcatcaaggg ctcactccgg aagaaggagg
    14041 caatcgagga ggtgctgctt ggcgaagaca ggacggccaa ggtgatctcc cttgtacgga
    14101 gggcgatgct cggacgcctc acgctcccct gcgatttcag cagcaccgag cgtgcgctcg
    14161 tgctggttgc cggccctccc gatgaacttg accggaaagg catcgagaaa gccaagagct
    14221 gggtggagga gaacatcgcg ggagtcgaag tacggggggg agattacccg gtgaacagca
    14281 atttcgtggc cgcggtcgtt gtccttgcca cggtaggaaa tgccccccgg atcaaggagc
    14341 ttatggagat cgcccgttcc acgagggacg atgcggcaaa gatcgaggag aagaaacctc
    14401 tctcatttga agaagagatt gaacctttgt ttgagtgata taccatgagg agttactgga
    14461 agtgtgtgtt aattctcgcc cttttgtgca tgttcatgca gggcgtggtt acagcgattg
    14521 cggttggaaa catcgccatc acgccctcaa cggatctcgt ctcggggcag acccaggtca
    14581 ggtcaacttt tgtcattaat ttcccggcaa gtggagggta cacgttcgac gatgcgaaca
    14641 gcctgcagct cgcaagcgag atggacactc ccagctggac gtatgcgatc gtcatggacg
    14701 ggattgagaa cccgtcgaag accgagatcg gccagaacat caatatcaac gggtggctcc
    14761 tctcgtaccc gtcaaagcgc gaactttcca tgaaagtgac catggaaggg gtcgcaccga
    14821 ccgttaccgg ttctgaggag aagatcgtca tcgcggtgag ggagcttgac agcaggggtg
    14881 caaagatcac aagctccgag gtggtcaaga agaagatggt gatcaacccg gcccagatac
    14941 aggtctccat caaccaggcc aaggaatccc tcgctgcgat gagggggcag attgaccagc
    15001 ttgccgcgtc cggaatcgac acatccccca tcgagcaaaa gtacagcgag gccagcaccg
    15061 cgatccacac tgctgaaggg gcctccgatt acaccagggc ccagcagtcg ctcaatactg
    15121 caaacgcggc cctggccgat gccgggacca tggtcaggga atttggcatc cagaagagta
    15181 tcaacgacgt ggacgcggtg atcgggcagg tgaacaccct gatcaccgat ttcagggtga
    15241 accggagcat gagttccgat gcccgcctca cgccaatcat tctcgcctat gacaatgcag
    15301 cgaacatggt cagcgcggca aaagacgaac tctccaggaa agagtacgac caggcgacga
    15361 gtaaagcgaa tgatgccagg gtgaaggcgg acgaggccct cgctgaggct ctcaagctga
    15421 aggagcaact tgatgcaaat ccccttgctg cggctgggac catgtttgca ggagtgctaa
    15481 ctggagggat tgtgatcatc atcgtggttg cggtcattgc ggtgattgcc gttctcgggc
    15541 tcctcttctt caggcgtcgc cggaaatggg acgagctcgg ttaaatccct cttttttcgt
    15601 gcgttcttct ctcaggggcg ttgtagtatt ccctgagtgc cttccaagtc ttttgtgggt
    15661 tgggattttt tcgctacagc gctggggcga aagccccagg agtatcatcg tatgagaggt
    15721 ctggtcgttt atttatgagg taacccgaat ttatcattac atatgaccgg agggggagaa
    15781 ttccctctcc cgacctcccc caggatgcga taacccgtgg gcagggttgt atggagcctc
    15841 tttccgaatt ctgccacaga taccggcctc atagtgatta ggcgatcatt cgtacgagaa
    15901 tcaggtaaag ggaacaaatg gagaagagat ccctgccttc tgata
//
LOCUS       DAOQYR010000048        15888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_4118_length_15888_cov_7.898250, whole genome shotgun sequence.
ACCESSION   DAOQYR010000048 DAOQYR010000000
VERSION     DAOQYR010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 15888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15888
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4118_length_15888_cov_7.898250"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="PKM87_06955"
     CDS             complement(<1..493)
                     /locus_tag="PKM87_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose 1,6-bisphosphatase"
                     /protein_id="HNQ29844.1"
                     /translation="MPKTTVSLIKADIGSFPGHSRTHPLILEKAARMLKEQEGKLLID
                     SFVTHCGDDLELIMTHTRGEDDAEIHALSWKVFTECAKVAKEMKLYGAGQDILSDAFS
                     GNVKGMGPGVAEMEFEERGSDPILLFMADKTEPGAWNFYLYKMFADPFNTAGLVIDPQ
                     MHDG"
     gene            complement(585..1028)
                     /locus_tag="PKM87_06960"
     CDS             complement(585..1028)
                     /locus_tag="PKM87_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29845.1"
                     /translation="MTEKPKDTERTLEEMLRHLMQAAQGGGDSRPLFIGMKIIVPPAG
                     DSPTPPQKARGDSTESPIEVHRVGDRVMLVTEMPGMTPENIQVMFRGDRVFIWARDQE
                     RHYQGSATVPPSQKGSEEISFRHGVLEVSYLPVEEKSEGDSSPHS"
     gene            complement(1075..3456)
                     /locus_tag="PKM87_06965"
     CDS             complement(1075..3456)
                     /locus_tag="PKM87_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HNQ29846.1"
                     /translation="MTTTDSIEVIVKEAARDDAGRGIARLSIETMRALGVVSGDAIEI
                     QGKKKATAIVWPGFSQDTGQAILRIDGTVRGNAGTGVDERVRVRKVEVGYAKKVVINP
                     TQPIRLLQGEQYLSRLLRGRAVMEGQTVRVDVIGNPLTLVITKVSPKGVAIVTEDTQI
                     ELKETPYEPAEKKKGETADVHYEDIGGLSRELEMVREMIELPLRHPELFERLGIEPPK
                     GVLLYGPPGTGKTLIAKAVANEVDAHFVSLSGPEIMSKYYGESEGKLREVFDEAQENA
                     PAIIFIDEIDSIAPKREDTKGEVERRVVAQLLSLMDGLKSRGQVVVIAATNIPDAIDP
                     ALRRGGRFDREIEIGIPDKKGRMEIFQVHSRGVPLAEDVKLESYADTTHGFVGADIAL
                     LVKEAAMHALRKILPKLDLDQEIPSELLDQLKVTNADFDEARRHVEPSAMREVLVEVP
                     DVTWEDVGGLEEVKQELREAVEWPLRYPQVFAKLGTKPPKGILLFGPPGTGKTMLAKA
                     VANESECNFISVKGPELLSKWVGESEKGVREIFRKARQASPAIIFFDEVDALVPKRGT
                     YMGSSHVTESVVSQILTELDGLEELKDVTVIGATNRPDMLDPALMRPGRMERHIYVPP
                     PDAESRKKIFEVYMKDADTILTGDVKIDDLVAATEGYVGADIEALVREAKLGAMREFI
                     TVMAEKSEQEMAEAVVNVRITRAHFEEAAKKVKGSMDRESLENAERQAWEILYNQEQR
                     TILENAVAAMKQAELRRDGEYDTRGLREQTYRRVKDWNEIKRLTQGLEEELRA"
     gene            3736..5013
                     /gene="serS"
                     /locus_tag="PKM87_06970"
     CDS             3736..5013
                     /gene="serS"
                     /locus_tag="PKM87_06970"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNQ29847.1"
                     /translation="MLDLKFIRENPDAVRQDLARRGDEEKRAWVDDLIENDRRSRVLK
                     VQADELRRRRNSIAREINEERKAGRDTGALKKEASELPGRIREIEAELDEITATVRFY
                     LMRLPNILHDSVPVGKDDTENVELRRVGSPRTPGFPLASHGQLASDRGWADFERAARA
                     AGAGFYYLKGNLALLDLALQRFAIDLLAEKGFVPVIPPYMINRRSYEGVTDLSDFEKV
                     MYKIEDDDAYLIATSEHPIGAMYQDEIFEERDLPLRLAGISPCFRREIGSHGLDTKGL
                     FRVHQFHKVEQFVFCRPEDSWEIHEELLANAEEIFSSLGLPYRVVNICTGDIGTVAAK
                     KYDIEVWMPREEAYREVVSCSNCTSYQAVRANIRVRDPHDFESKRFLHTLNSTAVATT
                     RTIRAILENYQEEGGAVMVPPALRPYMNDLEYL"
     gene            5261..5392
                     /locus_tag="PKM87_06975"
     CDS             5261..5392
                     /locus_tag="PKM87_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29848.1"
                     /translation="MPYHWKKKIDVDETIVVVKSVLTAEKELPNWLINTIYGAIARL"
     gene            complement(5563..6129)
                     /locus_tag="PKM87_06980"
     CDS             complement(5563..6129)
                     /locus_tag="PKM87_06980"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNQ29849.1"
                     /translation="MSSGKQEIRARAREVRAAIPAEQRVALSRAIREQVLALLDGTDP
                     VLVYASKPPEVDTLPLISTMISRGTRVVVPIIEKEERTLRLSYLDDAALLSVSTFSVP
                     EPIGHEVPADPGEIPVVIVPMIAYDAKGHRLGYGAGYYDRFLSRYPHMRKVGVAFSCQ
                     EVDSIPADENDVCMDIIVTECGITWIKE"
     gene            complement(6278..6583)
                     /locus_tag="PKM87_06985"
     CDS             complement(6278..6583)
                     /locus_tag="PKM87_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29850.1"
                     /translation="MVLLDGQRTSPVLPSLSTHCGANAAGGMCRNTWQTNCNAGTNLV
                     IPRHEKKVPGRECLEVGDVCSVHEAIHLFRAGVPRCPGCWSSRATPDPGCRRVVGGM"
     gene            complement(6764..7366)
                     /locus_tag="PKM87_06990"
     CDS             complement(6764..7366)
                     /locus_tag="PKM87_06990"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HNQ29851.1"
                     /translation="MDTLETIKGFLMQPVESFRKVRGTSLGDAIKYFLILVIINVILT
                     MIVDLVFASAILSTLTETLGQMGMGEVFLIETIGMVVLAIILIILMLVLLFVVAGWLH
                     LFVYLLGGRKGYAETAKALIFGSTPYMLIGWIPLIGLFIGGIWSLILGILGIRELHQV
                     STGRAAGAVVISAIILFIVVVLVAAWFIISLVSVEPVLVT"
     gene            7514..8728
                     /locus_tag="PKM87_06995"
     CDS             7514..8728
                     /locus_tag="PKM87_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29852.1"
                     /translation="MAIPMRHLALLAVVVLFVAVLPASAAHGGGSGSSSSHGGGSGSM
                     MPAGFGNSGMDSTGPASHGPGETAARNQAGQEGGGRSPSQGGVQAGGENRAIAGRAAQ
                     EWSPGGGGAGEAGSPKGPGGVLSFGRIGTVQPSSGDDALPAHPSGRSRGPPSHMQPNG
                     PGTGAGRLPCGPAQTAEPLSGATGPSDPAPSPSASPRSRRDGNDEESAPEPAGVSPAP
                     FFFLPFLGFRRITGKNVLAHDLRGRVYRAIASHPGIDALSLAQLLETNPSTLRYHLFT
                     LLRAEKITAFSRPGVVRYYPNQGMYPPFLQCLLHSLWTDTRREIIVLLWHTPGMTRTQ
                     LAVALACAGPSVTRQMQGLAEDGIVENRGTGRSNHYHLTAAAVEALVILGPRAGGPAL
                     PALAAPALPSAA"
     gene            8844..9176
                     /locus_tag="PKM87_07000"
     CDS             8844..9176
                     /locus_tag="PKM87_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29853.1"
                     /translation="MMRKTLALCLLLGFVLVPATVMAAGGGGQGRSGAQGGAIALLDQ
                     ADGTDAAAGQYQFCAQHGSNDVQKAGCGNMLRIRSCTGEGEMSQAKTMARNQTRLRDG
                     SCGNCPPR"
     gene            complement(9237..9368)
                     /locus_tag="PKM87_07005"
     CDS             complement(9237..9368)
                     /locus_tag="PKM87_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29854.1"
                     /translation="MEQSVMIVAIFVGAGLLAYVIVPGLIVIWDRVLDAVEERKGED"
     gene            complement(9406..9909)
                     /locus_tag="PKM87_07010"
     CDS             complement(9406..9909)
                     /locus_tag="PKM87_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29855.1"
                     /translation="MADFVQKSTTKTAARVLAAPIADVTTFAGIVQSVIDTNPFGCTS
                     QEVGGVTFDPVSKSREAYTARILYQDDDGKTVGQVTARSGSVAGFNAGIAEIMGNAEL
                     TAAMEGDPVRDAEHERYLCALRCHDPSGEVYYVTFARDQVRISSYADDVIVGLVEAWA
                     DTVPALA"
     gene            10130..10660
                     /locus_tag="PKM87_07015"
     CDS             10130..10660
                     /locus_tag="PKM87_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HNQ29856.1"
                     /translation="MDVEHMNLSRFDEMEKGELVSYLGFLLWHYRVVDAFWFIFLAEE
                     YGQPVAERINEQVWGRVASMAARDLKARFGITDTGLKGFVQALRLFPWAILVGYQIEE
                     NADEVVIRVPSCPTQEARLRRGLGEYVCREMHRAEFTAFAHEIDPRIRVECLFAPPDP
                     HPPDMFCMWRFTLSNE"
     gene            complement(10665..11003)
                     /locus_tag="PKM87_07020"
     CDS             complement(10665..11003)
                     /locus_tag="PKM87_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29857.1"
                     /translation="MTTTPRARPVEFEARSVLDSLGYHALRFNGSGSPVNLVGIDDHE
                     VLLVQVRRERLPYAGIREVNAKYRADMDRLRAIGGPRCCTKEIWVCSGHEGFRFYEVF
                     PGGLMEIERP"
     gene            complement(11233..11469)
                     /locus_tag="PKM87_07025"
     CDS             complement(11233..11469)
                     /locus_tag="PKM87_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29858.1"
                     /translation="MYPLLLAGSCGIYRNGATGRSISRRVAAFLHAVVVTIGILLGVA
                     VAGPENAEGTGGIATGPLSGPASPQPFTISPADR"
     gene            11775..11954
                     /locus_tag="PKM87_07030"
     CDS             11775..11954
                     /locus_tag="PKM87_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29859.1"
                     /translation="MDWKESRPCLPGHLLSKPVPGHRERGGPPPRPRSHLVPAMAHLL
                     VFPCSHPGNEGAMAR"
     gene            11970..14906
                     /locus_tag="PKM87_07035"
     CDS             11970..14906
                     /locus_tag="PKM87_07035"
                     /inference="COORDINATES: protein motif:HMM:NF027952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNQ29860.1"
                     /translation="MKRQSHTPWLCAIFVVAAVFYLVPSAMAASAAVLTLSVSPAVVT
                     SGEQVTLEGALSDASTGALIPMQIITVQSSPDGVTWTRVLSTISRTGQFQVTRLMRDP
                     GTYYFRVQYPGSRTYAAATSPPVVVTVKSPSGQKASSLSIYAAPHILAQGGTVTISGT
                     LVASQGGRGIQDRPVIVFSSSDGTSYSPVGTAITDQSGRYSHSTQMNTPGRFFFKAVY
                     NGDSSYTASASAVVAVDVTAVQQKATTLSIVASNSSLSLGQSVNLTGVLKETYGGIRI
                     PDSPVTVQFSFDGGAWSVLGQRTTDRNGEYLAVHTPSKAGKYLYRATYSGSTTHAPSE
                     SPSVTVNVTSLPGPGDSNLSIQVSPKSLGRGGTVTISGNLTAVKSGKGIPGRAVILFS
                     SSDGNIYAPLGVATTSADGKYSSSTQMNTPGRFFFKAVYNGDGTYTASASPVVAADVS
                     DLQQKSTALSIRPSSFTFTLGQSVDLAGVLAEAGTGRRIAGSAVGIQYSLDGAAWNSL
                     GQKITDGNGEYLVVHTPSKAGTYQYRATYSGNATYAPSASPYVSVIVRAPSGPGASAL
                     SINATPRDISPGGTVTISGNLTAAINGKGIPFRSIIVLSSSDNTSFSPLGVVTTSPDG
                     KYTSSTQLNTPGRFFFKAVYNGDSTYTASASPVVAVNVTGPQQKATTLTIGAAPVSLS
                     LGQSVEVAGVLKETQGGTRIPGAVILVQYSVDAGAWSTLGQRTTDEKGEYTLAHTPSM
                     AGTYKYRATYAGSTTHASSASATVTIRVTGMPLAHGTMLTIKSTPASPLRGEPYTLSG
                     LLRDTSTGKGISGKAVRVERSTDLGTWTLVGMAITQTGGSYSVSEVQQAGGRFSYRAA
                     FSGDRSYLGSASPVIPVVIKRVSEVSLSASPVLIPPGGTVNCTGTLVDPQSGAALAGE
                     PVKVMISPENAHWTVFGTAVTGTDGSWKLASRIANAGSYYLAARFEGSASYDEDWSNS
                     VGITVR"
     gene            complement(14949..15021)
                     /locus_tag="PKM87_07040"
     tRNA            complement(14949..15021)
                     /locus_tag="PKM87_07040"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14985..14987),aa:Gln,seq:ttg)
     gene            complement(15293..>15888)
                     /locus_tag="PKM87_07045"
     CDS             complement(15293..>15888)
                     /locus_tag="PKM87_07045"
                     /inference="COORDINATES: protein motif:HMM:NF036704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharyl transferase, archaeosortase A
                     system-associated"
                     /protein_id="HNQ29861.1"
                     /translation="LTPLTLIDAPYYHTMIARLHALDGSMAVPDRAYYLEYQEKAGSV
                     PVITKIEHLSYDAARQKKNGFPGSGGGEISAAVLSSSLSAPLSVVPALQHYRLIYESP
                     HRDPVDNLSDVKIFERVKGARIAGEGTIELVLVTNQGRTFTYRQESRNGTFIVPYSTV
                     DNPYPVRAEGPYRIAGMSLAYDVSEEDIKEGRQVTAR"
BASE COUNT         2975 a         4953 c         4844 g         3116 t
ORIGIN      
        1 acccgtcgtg catctgcggg tcgatgacga gacccgcagt gttgaacggg tcggcaaaca
       61 tcttgtagag gtagaagttc caggcgccgg gctcggtctt gtcagccatg aagaggagga
      121 ttgggtcgga ccctcgctcc tcgaactcca tctccgcgac gcccgggccc attcccttca
      181 cgtttcctga gaatgcgtcc gagaggatgt cctggccggc gccgtaaagc ttcatctcct
      241 ttgcgacctt cgcgcactcc gtgaacacct tccaggagag ggcatggatc tcggcgtcgt
      301 cctctccccg tgtatgggtc atgatcagct cgaggtcgtc gccgcaatgc gtcacgaagg
      361 agtcgatcag gagcttgccc tcctgctcct tgagcatcct tgcagccttc tcgaggatga
      421 gcgggtgggt ccgggagtgg cccggaaagc tcccgatatc agcttttatc agtgaaaccg
      481 tggtctttgg catgaaaatc tcctggtgtc agttcttaca tactaataga tacaggagat
      541 aaaaaactgt gggtttttta aaaaaacgtt ggcggtgctc tcgatcagga gtgaggagaa
      601 gagtctcctt cgctcttctc ttccaccggg agataggaga cctcaagcac cccgtggcgg
      661 aaggagatct cctcggatcc cttctgtgac gggggaaccg tggcactgcc ctggtaatga
      721 cgctcctggt cgcgtgccca gatgaagacc cggtcgcccc ggaacatcac ctggatgttc
      781 tcgggcgtca tccccggcat ctcggtgaca agcatcaccc tgtctcctac ccggtgcacc
      841 tcgatcgggg actccgtcga gtccccgcgg gctttctggg gcggggtcgg agagtcgccg
      901 gccggcggaa cgatgatctt catccctata aatagcgggc gggagtcccc gccgccctgc
      961 gcagcctgca tcaggtgtcg cagcatctct tcgagggttc tctctgtgtc ttttggtttc
     1021 tctgtcatct cgctgctcgt tctggctgaa tggacgagag ggaggggggc ccggtcaggc
     1081 ccggagttcc tcctccagcc cctgcgtcag ccgcttgatc tcgttccagt ccttgaccct
     1141 ccggtaggtc tgctcgcgga gccctcgggt gtcgtattcc ccatccctcc ggagctctgc
     1201 ctgcttcatg gccgcgaccg cgttctcgag gatagtgcgc tgctcctggt tgtagaggat
     1261 ctcccatgcc tggcgctcgg cgttctccag ggactcgcgg tccatcgacc ccttgacctt
     1321 ctttgccgcc tcttcgaagt gcgcccgggt gatgcggacg tttacgaccg cttctgccat
     1381 ctcctgctcg ctcttttcgg ccatcacggt gatgaactcg cgcatggccc caagcttcgc
     1441 ctcgcggacg agggcctcga tgtcggcccc gacgtagcct tccgtcgcgg ccaccaggtc
     1501 atcgattttc acgtccccgg tcaggatggt gtccgcgtcc ttcatgtaca cctcgaatat
     1561 cttcttcctg ctctccgcat ccgggggcgg gacgtagatg tgccgctcca tcctcccggg
     1621 gcgcatcagt gccgggtcca gcatgtcggg gcggttggtg gccccgatca cggtcacgtc
     1681 tttcaactcc tcgagcccgt cgagctcggt caggatctgg ctgaccacgc tctcggtgac
     1741 gtgcgaggag cccatgtagg tgccgcgctt cggtacaagg gcgtccacct cgtcgaagaa
     1801 gatgatcgcg ggcgaagcct gacgcgcctt gcggaagatc tcccgcaccc ccttctcgga
     1861 ctcccctacc cacttcgaga ggagttcggg gcctttcacc gatatgaagt tgcactcgct
     1921 ctcgtttgcg accgcctttg cgagcatcgt cttcccggtc cccgggggac cgaagaggag
     1981 gatgcccttc gggggctttg tgccaagctt cgcaaagacc tgtgggtagc ggagcggcca
     2041 ctccactgcc tcccggagct cctgcttcac ctcctcgagg ccgcccacgt cctcccaggt
     2101 gacatccggg acctccacga gcacctcgcg catcgcgctc ggctcgacgt gcctcctggc
     2161 ctcgtcgaag tccgcgttcg tgaccttgag ctggtcgagg agctctgatg ggatctcctg
     2221 gtcaaggtcg agtttcggga ggatcttccg gagggcgtgc atcgcggcct ccttgacgag
     2281 gagggcgatg tcggcaccca cgaacccgtg ggtggtgtcg gcatagctct cgagttttac
     2341 gtcctctgcg agcggcacgc cgcgggagtg gacctggaag atctccatcc tccccttctt
     2401 gtcggggatg ccgatctcga tctcccggtc gaagcggcct ccgcggcgca gcgcggggtc
     2461 gatggcgtcg gggatgttcg tcgcggcgat gacgaccacc tgtcccctgg acttgaggcc
     2521 gtccatcagg gagaggagct gggcaaccac ccgccgctcc acctcgccct tcgtgtcctc
     2581 ccgcttgggg gcgatcgagt cgatctcgtc aatgaagatg atcgcaggag cattctcctg
     2641 cgcctcgtcg aagacctcgc ggagcttgcc ctcgctctcc ccgtagtact tgctcatgat
     2701 ctcggggccg gagagggaga cgaagtgggc gtccacctcg tttgcgacgg cctttgcgat
     2761 cagggtcttg ccggtccccg gggggccgta gaggaggact cccttcgggg gctcgatccc
     2821 gaggcgctcg aagagctcgg ggtgacgaag cggcagctcg atcatctccc gcaccatctc
     2881 gagttcccgc gacagcccgc cgatgtcctc gtagtggaca tccgcggtct cccccttctt
     2941 cttctccgcg ggctcgtagg gggtctcctt gagctcgatc tgggtatcct cggtgacgat
     3001 cgcgacgccc ttcgggctca ccttggtgat caccagggtg aggggatttc caatcacgtc
     3061 cacccggaca gtctggccct ccatcaccgc ccggcccctg agcaggcggg agaggtactg
     3121 ttccccctgg aggaggcgga tgggctgggt ggggttgatc acgactttct tggcataccc
     3181 cacctcgacc ttgcgcacgc ggaccctctc gtcgacaccc gtcccggcgt tgccacggac
     3241 agtcccgtcg atgcggagga tggcctgccc ggtgtcctgc gagaagcccg gccagacgat
     3301 ggcagtcgcc tttttcttgc cctggatctc gatggcgtct ccgctcacca ccccgagcgc
     3361 cctcatcgtc tcgatgctca ggcgggcgat gccccgtccg gcatcgtccc tggccgcctc
     3421 ttttacaata acctcgatgg aatcagtagt tgtcatggga atatggttcc tgtaatgttt
     3481 acttttagta agtgattcat attatttata cttattttat aaggaaggcc agatcgattg
     3541 gacagaaatc cttcctggac cttcaaaagg catggatcct tgaattttgg tgatttttca
     3601 tgaaaggagc caggtgtctt tttcccttcc ggtggacgtg acggtcgacc cgcaaaggca
     3661 atgcgttcca agcgcacacc taaactggag aggcaagagc ctggatcaac tgcggcgaga
     3721 aggtgcaata aagagatgct cgacctgaag ttcatacgtg aaaatcccga cgcggtgagg
     3781 caggacctcg ccaggcgggg ggatgaggag aagcgtgcct gggtggacga cctgatcgaa
     3841 aacgaccgcc gttcccgggt gctgaaggtg caggccgacg agctccgcag gcgccggaac
     3901 agcatcgcga gggagattaa cgaggagagg aaggcagggc gtgacacggg agccctcaaa
     3961 aaagaggcct ccgagctgcc ggggaggatc agggagatcg aggcggaact ggacgagatc
     4021 acggcaacgg tccgctttta cctgatgcgc ctcccgaaca tcctccacga cagtgtcccg
     4081 gtcggaaaag acgacaccga gaatgtcgaa ctccgccggg tggggtcgcc ccgcacgccc
     4141 ggattccctc tcgcaagcca cgggcagctc gcaagcgacc ggggctgggc ggacttcgag
     4201 cgggcggccc gggcggcagg tgcgggcttt tattacctga agggaaacct cgcgcttctc
     4261 gacctcgcgc tccagcggtt cgccatcgac ctgctcgcag agaaggggtt cgtgccggtc
     4321 atccccccct acatgatcaa ccggaggtcc tacgagggag tgaccgacct ttctgacttc
     4381 gagaaggtca tgtacaaaat cgaggatgac gacgcatacc tgatcgcaac gagcgagcac
     4441 cctatcgggg caatgtacca ggacgagata ttcgaggagc gcgacctccc gctccggctt
     4501 gcagggatat ccccgtgctt caggagggag atagggtcgc atggcctcga tacaaagggc
     4561 ctcttcaggg tgcaccagtt ccacaaggtg gagcagttcg tcttctgccg gccggaggac
     4621 tcgtgggaga tccacgagga actgctggca aacgccgagg agatcttctc gagcctcggc
     4681 cttccttacc gggtggtcaa tatctgcacc ggagatatcg ggactgtcgc cgcaaagaag
     4741 tacgacatcg aggtctggat gcccagggag gaggcttacc gggaggtggt ctcctgctcg
     4801 aactgcacct cataccaggc ggtgcgtgcc aacatccggg tccgcgaccc gcacgacttc
     4861 gagtcgaagc ggtttctgca tactctcaac tcgactgcag tagcgacgac gcggacaatc
     4921 cgggcaatcc ttgagaacta ccaggaagag ggaggggcag tcatggtgcc ccccgccctc
     4981 cggccctaca tgaacgatct cgaatacctc tgatccgttc accccgagac ccggacaacc
     5041 acttttttta gcggcgcctg ggatccatga agatgcgggt cttttcgaag aagggcatcc
     5101 gccacctgaa aagagaagac aacctccatc attctcgacg ggcgggagta aaaacgagag
     5161 gggggattca ttccgggctg tccctttgga gggatggcat ttgtcggtat ccacgtgttt
     5221 agagtttgca tcgcgtactt caccattcgg agggccaaaa atgccatacc actggaagaa
     5281 aaagattgat gtggatgaga ccatcgtcgt cgtcaaaagc gtgctgaccg cagaaaaaga
     5341 actccccaac tggctcatca atacgattta cggggcgatt gcgcgactct gatcccgcca
     5401 tggtgaaata cttctatgca gaagtgaaaa aatacgctcc ggcgaccatg aaatatttcg
     5461 aggaaggttc tacgaggtct ccgatttcac aaccctctcc tttttcccca aataaccagt
     5521 tacccggact gccccgggcc tgttccggaa accggttcca gatcactctt tgatccaggt
     5581 gatcccgcat tccgtgacga tgatgtccat gcagacatcg ttctcgtcgg ctggaatgga
     5641 gtccacctcc tggcaggaga aggccacccc cactttcctc atgtgggggt agcgtgagag
     5701 gaaacggtcg tagtaccctg ccccgtatcc aaggcggtgg cccttcgcgt catacgcgat
     5761 catggggacg atcaccaccg ggatctcgcc ggggtcggct ggcacctcgt gcccgatggg
     5821 ctcggggacc gagaacgtgc tcaccgagag gagggctgca tcgtcgagat aagagagccg
     5881 tagcgttctc tcctcctttt cgatgatggg gaccaccacc cgtgtccccc gcgatatcat
     5941 ggtcgagatg agcgggagcg tatccacttc gggaggcttc gacgcgtaga cgagcacggg
     6001 gtccgtgccg tccaggagcg cgagcacctg ctccctgatg gcccgtgaga gcgcgaccct
     6061 ctgctccgcc gggatggcag ccctgacctc ccgcgccctg gcgcgtatct cctgcttccc
     6121 gctgctcatt ctgctgtact gtaccattcg tcccggaagg agaattaagg gtgcggtccg
     6181 tcaccgggaa gcgggttgcg acagcgaggg aagaggagtg tttccgactt tacaaaactt
     6241 tacttattaa gtgctacgta attaacaaag tttattacta catcccgcca accactctcc
     6301 tgcagccggg gtcgggggtt gcccggctcg accagcaccc ggggcagcga gggacgccgg
     6361 cccggaagag atgaatcgcc tcatgaacac tgcatacatc ccccacctcc aggcattccc
     6421 ggcctggaac cttcttttcg tgacggggga tcaccagatt cgtccccgca ttgcaattcg
     6481 tctgccaggt gttgcgacac atgcctcccg cggcgtttgc accgcaatga gtggagaggg
     6541 acggaagcac cggggaagtc cgctgcccgt ccaataacac cacatgattc tcttcgagac
     6601 acacgcatac gcattcatga aaatggtgca gccattttca aagtaaaatg ggacgccggc
     6661 cagggtaacc ggcctctctt cgggatctcc tctgcgcact gatcccctca cggcctgccg
     6721 cacctggagt tcatgcccca ccgatcagga aaaaaggggg ggttcaggtg acgagcacgg
     6781 gctcgacgct gacaagcgag atgatgaacc aggcagccac gagcacgacg acaatgaaga
     6841 ggatgatcgc ggaaatgact accgccccag ctgcccgccc cgtcgagacc tggtgcagtt
     6901 cccggatccc gagaatcccg aggatcagcg accagatccc cccgatgaag agcccgatga
     6961 gtgggatcca cccgatcagc atgtagggtg tggacccaaa tatcagggcc tttgcggtct
     7021 ctgcatagcc cttccgtcct ccaaggagat acacgaagag gtgcagccag cctgcgacga
     7081 caaagaggag gaccagcatc agtatgatca ggatgatcgc gagcaccacc atcccgatcg
     7141 tctcaatcag gaagacctcc cccatgccca tctggccaag tgtctcggtg agcgttgaga
     7201 gtattgcgct cgcgaacacg agatccacga tcatggtgag aattacattg atgatgacaa
     7261 gaatgaggaa gtacttgatc gcgtccccaa gcgaagtccc cctgaccttg cggaaggact
     7321 cgaccggctg catgagaaac cctttgattg tctcgagtgt atccatagac accactatgg
     7381 ctggtatgat cgcaggatat aagaagagtg cacctttttt ccccggccat tccccccacg
     7441 ttcaccccca tctccccgtg tagctgccgg taccagagtg tatatatcgg acacggccca
     7501 cctgatcacc aaaatggcca ttccaatgcg gcatcttgcg ctgctggcag tcgtcgtact
     7561 cttcgtggca gtcctcccgg cgagcgcagc ccatggcggc ggttccggct cttcttcatc
     7621 acatggcggg ggcagcgggt ccatgatgcc ggcggggttc gggaattccg ggatggactc
     7681 cacgggcccg gccagccatg gcccgggtga aaccgcggcc aggaaccagg caggacagga
     7741 ggggggcggg cgctcccctt cccagggcgg cgtgcaggcc gggggcgaga accgggcgat
     7801 tgcaggccgg gccgcccagg agtggtcccc agggggcggt ggtgccggag aggccgggtc
     7861 tccaaaggga cccggcggtg tcctttcctt cgggaggatc gggacggtgc agccatcttc
     7921 aggcgacgat gcactccctg cacatccttc cggccggagc agggggcctc cctcgcacat
     7981 gcagcccaat ggaccgggga cgggcgcggg acggctcccc tgcggccctg cacagactgc
     8041 ggagccgctt tccggggcga cggggccgtc cgacccggcc ccgtccccct ccgcctcccc
     8101 gagatcgagg agagacggaa acgacgagga atccgccccg gaacccgccg gggtttcgcc
     8161 cgccccattc ttcttccttc cattccttgg cttcaggagg ataaccggga agaatgtcct
     8221 cgcacatgac ctgcggggga gggtgtaccg tgccatcgcg tcgcatcccg ggatcgatgc
     8281 cctctccctt gcacagctcc tggagaccaa cccgagcacg ctccggtacc acctcttcac
     8341 attgctgcgg gcagagaaga tcacggcgtt ctcccggccc ggcgtagtgc ggtattaccc
     8401 gaaccagggg atgtaccccc cgttcctcca gtgcctcctc cattccctct ggaccgatac
     8461 caggagggag atcatcgtgc tcctctggca cacccccggg atgaccagga cccagcttgc
     8521 agttgcgctc gcatgcgcgg ggccgtcggt cacccgacag atgcagggcc tcgccgagga
     8581 cgggatcgtg gagaaccggg gcacgggcag gtcgaaccac taccatctca ccgcggccgc
     8641 ggtcgaggca ctcgtaattc tcgggccgag ggcaggaggc ccggcgctcc ctgcactggc
     8701 cgcgccggcg ttgccttccg ccgcgtgacc gttctcctga tgagtagctg cccgtgcgag
     8761 agatcccttg aatacccctg ccccatgctc ttgtgaggcg ggtcgcccgg cccggtgccg
     8821 gcgacccggc aacggaggta acaatgatga gaaagacact tgcgctctgc ctcctcctcg
     8881 gcttcgtcct cgtgcctgcc acggttatgg cggcaggggg aggcggccag gggaggtccg
     8941 gcgcacaggg aggagcgatc gcgctcctgg accaggcaga cgggaccgat gcagcagcag
     9001 gccagtacca gttctgcgcg cagcacgggt caaatgacgt tcagaaggca ggttgcggga
     9061 atatgctccg catccgctca tgtaccggcg agggggagat gtcgcaggca aagaccatgg
     9121 cgcggaacca gacacggctc cgggatgggt cctgcggaaa ctgccccccg cgctgattgg
     9181 aagtgtgaaa aaattacgaa tttttttacc ggtgaagggg gagagtggga ggatttttag
     9241 tcctctcctt ttctctcttc cacggcgtcc agcacccggt cccagattac gatcaggcct
     9301 ggcacgatca cgtaggcaag gagcccggcc cctacaaaga ttgccacgat catgacggac
     9361 tgttccatgg ttgtctccat gagaccggtc catatcgcgt ggcaatcagg ccagcgcagg
     9421 gacggtgtcg gcccatgcct ccaccagccc cacgatgacg tcgtcagcgt atgaggagat
     9481 ccgcacctgg tcgcgggcga aggtgacgta gtagacctct cccgaagggt cgtggcaccg
     9541 cagggcgcag aggtagcgct cgtgttcggc gtcccggacg gggtcgcctt ccatggccgc
     9601 ggtaagttcc gcgtttccca tgatctctgc aatgcctgcg ttgaaccccg ccacgctccc
     9661 cgagcgggcg gtgacctggc ccacggtctt tccgtcgtca tcctgataca ggatccttgc
     9721 tgtgtacgcc tcgcggctct ttgacacggg gtcgaaggtc acgcccccaa cctcctgcga
     9781 ggtgcatccg aaggggttcg tgtcgatcac gctctgcacg atcccggcaa aggtggttac
     9841 atcagcgatc ggtgcggcca gcacgcgggc cgcggtcttg gtggtgctct tctgcacaaa
     9901 gtctgccatt ctcttctttc ctgccccccc acttgagagg ggcacatacc cggtgggagg
     9961 gaggtaatca aggagattgg gcacagggga taaatggaga ccaccatagc gtctttttgc
    10021 caatatatgg cttgtgagtt gcagattgaa cgatcggtgg caattcacgg agaatattcc
    10081 ccggagacca aaaagggaaa tgtgatgtgg tcccggggcg atctggggca tggatgttga
    10141 acatatgaac ctgtcacggt tcgatgagat ggagaaggga gagctggtct cgtacctcgg
    10201 gttcctgctc tggcactacc gggtggtgga cgcgttctgg ttcatcttcc ttgcagagga
    10261 gtatgggcag ccggtcgctg agcggatcaa cgagcaggtg tgggggaggg tcgcctcgat
    10321 ggcggcaaga gacctgaagg cgcggttcgg gatcactgat acagggctga aggggttcgt
    10381 acaagccctc cgcctcttcc catgggcaat cctcgtcggc taccagatcg aggagaacgc
    10441 ggacgaggtg gtcatcaggg tgccctcctg ccccacccag gaagcgcgcc tgcgccgcgg
    10501 gctcggcgag tacgtctgcc gggagatgca cagggcggag ttcactgcgt ttgcacacga
    10561 gatagacccg cggatcaggg tggagtgcct cttcgctccg cccgacccgc accctcccga
    10621 catgttctgc atgtggcgct tcactctctc aaatgaatga gggttcaggg ccgctcgatt
    10681 tccatcaggc ctccggggaa gacctcgtag aaccggaacc cctcatggcc cgagcagacc
    10741 cagatctcct tcgtgcagca ccgcggaccg cctatcgcac gcagccggtc catgtcggcc
    10801 cggtattttg cattcacctc ccggatcccc gcgtaaggca ggcgttccct ccggacctgg
    10861 acgaggagga cttcgtgatc atcgatcccc accaggttca cggggctgcc tgacccgttg
    10921 aaccgcagcg cgtggtatcc cagggagtcg aggacggacc gcgcctcgaa ctccacgggg
    10981 cgggccctcg gggtggtggt catggtgcgg ttccccccat ggcgtggcaa atggtgcgcc
    11041 ggggtgcatg cgcaggtcgt gcgatgcggc aggtgctgcc tttcagatgg gtatgcgaga
    11101 gtggattgtc gggctgggtg agaggggaca tgaacgggat cccccttttt cgggtatgca
    11161 ggtgtgaatg cagtgcagag tgcatgaccc cgggattttc ctgggatttt tgcgcgggaa
    11221 gagaagcggc gatcacctat cggcggggga aatggtgaat ggctgaggag aggcggggcc
    11281 tgacaggggg cctgtggcga tgccgcccgt cccttccgca ttctccggcc ccgccactgc
    11341 aaccccgaga agtatcccga tcgtgacgac caccgcatgg aggaacgcgg ccacacgtct
    11401 ggagatgctc ctccctgtgg ccccattcct gtaaatgccg cacgatcctg cgagtaaaag
    11461 gggatacatg tcctaaaaaa tagtgttttc catatccatt tgaatattgc ggtggaacga
    11521 ccggaaccgg cctggaaatg cccggttatt ctcccgtgta ctcctccttc aatgggcgtt
    11581 ttgtgaccgg gatcgtgatc gcgcgtggcc cgtcccgccg ggactcaaaa aaactgtgga
    11641 aatggcgtat tttttggatc ctctgtgatg gtgccgcagg gaacgaaaac gaatggtctg
    11701 cagggcattc ccggtttcgg accggccggg acgaatcgga tatttatata tagtccaaag
    11761 tttttccact tctcgtggac tggaaggagt cgcgcccctg tcttcccggg cacctccttt
    11821 caaaaccagt gcctgggcac cgggagagag gcggcccacc tccccggccg cgttcgcacc
    11881 tggtgcctgc catggcgcac ctcctggtct tcccgtgcag ccatcccggg aacgagggcg
    11941 ccatggccag atgacaaggt ggtaatgaaa tgaaaagaca atcccatacc ccgtggctgt
    12001 gtgcgatatt cgtcgttgca gccgtctttt acctggtgcc atccgcaatg gcggccagtg
    12061 ctgccgtgct cactctctct gtgtcaccag cggtcgtgac ttccggagag caggtaaccc
    12121 ttgaaggagc cctctcggac gcctcgacgg gcgccctgat acccatgcag ataatcacgg
    12181 tccagtcttc tcccgacggg gtgacctgga ccagggttct ctccactatc agcaggaccg
    12241 ggcagttcca ggtcacgcgg ctgatgcggg accccgggac ctattatttc agggtgcagt
    12301 accccgggag caggacctac gcggcagcga ccagtccccc ggtggttgtg accgtgaagt
    12361 ctccttccgg gcagaaagcc tcgtccctct cgatatatgc ggctccccat atcctggcgc
    12421 agggaggcac ggtgacgatc tcagggaccc tcgttgcatc ccaggggggc agagggatcc
    12481 aggaccgacc ggtgatcgtc ttctcttcat ccgacggtac gagttactct cccgttggca
    12541 cggcaataac tgatcagagt gggaggtact cccacagcac ccagatgaac acccctggcc
    12601 gcttcttctt caaggcggtc tacaacgggg acagcagcta cactgcctcg gcgagcgccg
    12661 tcgtggcggt ggatgtgacc gctgtgcagc agaaggccac gaccctctcg atcgtggcat
    12721 ccaactcatc gctctcgctc ggccagtctg tgaacctgac aggggtcctc aaggagacat
    12781 acggcgggat tcgcatcccc gattcccctg tgactgtcca gttctcattc gacgggggtg
    12841 cgtggagcgt gctcggccag aggacgacgg ataggaacgg ggagtacctg gccgtgcaca
    12901 ccccttcgaa agccgggaag tatctgtacc gcgccaccta ctccggcagc accacccatg
    12961 caccctcgga aagcccctcc gtgaccgtga acgtgacgtc gcttccgggg cccggagact
    13021 ccaacctctc gatccaggtc tctccaaagt cccttggcag gggagggacc gtgacgatct
    13081 ccgggaacct cacggccgtc aagagcggga agggcatccc cgggcgggcg gtcatcctgt
    13141 tctcctcatc tgacggcaac atttacgctc ccctcggcgt ggcaacgacc tctgctgacg
    13201 ggaagtattc aagcagcact caaatgaaca cccccggccg cttcttcttc aaggcggtct
    13261 acaacgggga cggcacctac accgcctccg caagccccgt cgtggcggcg gatgtcagtg
    13321 acctgcagca gaagtcaacc gccctctcca tcaggccatc ttccttcacg ttcaccctcg
    13381 gccagtccgt ggacctcgca ggggtgcttg cagaggcagg aacgggcagg cgcatcgcgg
    13441 gttcggctgt cggcatccag tactcccttg acggggctgc ctggaactcg ctcggccaga
    13501 agatcacgga cgggaacggt gaataccttg tcgtgcacac tccctcaaag gcggggacat
    13561 accagtaccg cgccacctac tccggcaacg ccacgtatgc gccctcggcc agcccctacg
    13621 tgagcgtgat cgtcagggcc ccctcgggcc cgggggcttc cgccctctcg atcaacgcga
    13681 ctccccggga catctccccg ggtggcacgg tgacaatctc cgggaacctc accgccgcca
    13741 tcaatgggaa agggatcccg ttccgttcga tcattgtgct ctcctcttcg gacaatacca
    13801 gtttctcccc tctgggcgtg gtgaccacct ctcctgatgg gaaatacacg agcagcaccc
    13861 agctgaacac ccctggccgc ttcttcttca aggcagtcta caatggggac agcacttaca
    13921 ccgcctccgc aagccccgtc gtggcggtga atgtcaccgg cccgcagcag aaggcaacga
    13981 ccctcaccat cggtgcggcg ccggtctcgc tctccctcgg ccagtcggtc gaggttgccg
    14041 gagtattgaa ggagacccag ggaggcactc gcatccccgg cgccgtgatt ttggtccagt
    14101 actccgttga tgcgggggcc tggagcacgc tcgggcagag gaccacggac gagaaagggg
    14161 agtacaccct cgcgcatact ccttcgatgg cagggaccta caagtacagg gccacttacg
    14221 ctggcagcac cacccacgca tcctcggcga gcgccactgt gaccatacgg gtcaccggga
    14281 tgcccctggc ccatgggacc atgctcacca tcaagtccac tccggcatct cctctgcgtg
    14341 gagagccgta tactctcagc gggcttctcc gtgacacctc caccgggaag gggataagcg
    14401 gaaaagcggt gagggtagag cgatcaaccg acctgggcac gtggaccctc gtcggcatgg
    14461 ccatcaccca gaccggcggg agctattctg tctcggaggt gcagcaggcg ggcggcaggt
    14521 tctcctaccg ggctgccttc agcggcgacc gttcatacct cgggtcggca agccccgtca
    14581 tcccggtcgt cataaagcgg gtctccgaag tgagcctttc cgcgtcaccg gtcctgattc
    14641 caccgggtgg aaccgtgaac tgcaccggga ccctggtcga cccgcagagc ggcgcagccc
    14701 tcgcaggaga gcccgtgaag gtgatgatct cgccggagaa cgctcactgg acggtctttg
    14761 ggaccgcggt caccgggacc gacgggagct ggaaactcgc gtccaggatc gcgaatgccg
    14821 gctcctacta ccttgccgcg aggtttgagg ggtccgcgtc atacgacgag gactggagca
    14881 actcggtcgg gattactgtc cggtaaccat cctttttttt acaccgggag ccacggtgcc
    14941 ccattcgata cccccgggcg gattcgaacc gccgtcgccg gatccaaagt cctgcatgat
    15001 tgaccgctac actacggggg tgcgggccct gttgaccgct acatcaccgg gaaaggtgtg
    15061 tagccggaca acgtcgtccc aataggaatt gcggcgcaga ataattaatt catcttggga
    15121 ggaggtctct cttcacttcg ggatcggggc cgccgcacag atcgatcccg gaattgccgg
    15181 ggcctttaca cagattattg tcaacgcagg aacgggtgcg gccattccgc gtctgccacc
    15241 atcccggtat cgaccgaaat tttttggggg aggaggccct ttccactgtt tctcaccgtg
    15301 cggtgacctg ccttccttct tttatatcct cctcgctgac gtcgtatgca agggacatgc
    15361 cggcaatgcg gtaggggcct tccgccctga cgggataggg attgtccacc gttgaatagg
    15421 ggacgatgaa cgtcccattt ctgctctcct ggcggtaggt gaacgtcctg ccctggttgg
    15481 tgacgagcac aagctcgatc gtcccctcgc cggcaatcct tgcacccttc acccgctcga
    15541 atatcttgac atcggagaga ttgtcgaccg ggtccctgtg gggggattca tagatgaggc
    15601 ggtagtgctg gagcgcggga accaccgaga gcggggctga aagggaactg ctgagcaccg
    15661 cggcagagat ctcccctccg ccggatcccg ggaacccgtt ctttttctgc cgggcggcat
    15721 cgtaggagag gtgctcgatc tttgttatca ccgggaccga acctgccttt tcctggtact
    15781 cgaggtaata ggccctgtca gggaccgcca tcgagccatc gagggcatgg agccgtgcga
    15841 tcatggtatg gtaatacgga gcgtcgatga gggtaagcgg ggtcaggg
//
LOCUS       DAOQYR010000049        15662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_4205_length_15662_cov_9.353687, whole genome shotgun sequence.
ACCESSION   DAOQYR010000049 DAOQYR010000000
VERSION     DAOQYR010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 15662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15662
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4205_length_15662_cov_9.353687"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(278..350)
                     /locus_tag="PKM87_07050"
     tRNA            complement(278..350)
                     /locus_tag="PKM87_07050"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(314..316),aa:Asp,seq:gtc)
     gene            complement(384..489)
                     /locus_tag="PKM87_07055"
     tRNA            complement(join(384..428,452..489))
                     /locus_tag="PKM87_07055"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(453..455),aa:Tyr,seq:gta)
     gene            620..2158
                     /locus_tag="PKM87_07060"
     CDS             620..2158
                     /locus_tag="PKM87_07060"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNQ29862.1"
                     /translation="MWGIVFFVDSPSPRRVTVFDTTLRDGEQTPGISFKFEQKLEIAR
                     QLSSIGVHAIEAGFPASSKGEMETVKAIADLDLESVVCGLARSRKEDVDACLDCGVDM
                     VHVFIPTSEVQRVHTIKKSCEEVLEITGEIVSYVREHCDLCMFSAMDATRTDLDYLVQ
                     VFRTAADAGATILNVPDTVGVYTPTAMKGLISHLAAEIRCPLDVHCHNDFGLAVANTI
                     TAVEAGASQVQVTVNGLGERAGNADLSSTVMILESIFGMQTGIKKERLVETSRLISRY
                     SGISIPPTYPIVGENAFSHESGIHSHGVIACAATFEPGIMTPEMVGHRRKLTLGKHVG
                     RHAVRQMLEEVHLDPSDSQLDRIVEKIKFISVKGKRVTDADLYEIAESEMGIGNGGRM
                     IEMDELAVMTGNHLIPTASVKAKIGGKEAVYSATGTGPVDAALKAVLGMLPMKVQLKE
                     FSIEAISGGSDAIGHVTIAVEDEQGRIFDASASGDDIVLASAEAMINALNMLHRLDPH
                     TGKK"
     gene            complement(2172..2534)
                     /locus_tag="PKM87_07065"
     CDS             complement(2172..2534)
                     /locus_tag="PKM87_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit beta"
                     /protein_id="HNQ29863.1"
                     /translation="MSSISPKMQNQIAQLQQMQQQMQTIAAQKNQYEMAVREARRATE
                     ELKEVPEENAVFMSVGSVMMQKKKEDVLVKLNDKIETLEIRIKSLEKQETMLQGKFEQ
                     LQTQIKQALEGKSSPGAA"
     gene            complement(2531..2833)
                     /locus_tag="PKM87_07070"
     CDS             complement(2531..2833)
                     /locus_tag="PKM87_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HNQ29864.1"
                     /translation="MPENERACEGEMGGMEHEAVFHFRCPHAETLFRSLSPEMAAELH
                     PRSRGECFMEGQGVLVLKVQARDCAALRAALNMWLRLVNVADEMQALASPAQKERS"
     gene            complement(3122..3256)
                     /locus_tag="PKM87_07075"
     CDS             complement(3122..3256)
                     /locus_tag="PKM87_07075"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit P"
                     /protein_id="HNQ29865.1"
                     /translation="MAGAYKCARCKQKVEIDVNVRCPYCGHRILFKERGAAIKELKAR
                     "
     gene            complement(3264..3554)
                     /locus_tag="PKM87_07080"
     CDS             complement(3264..3554)
                     /locus_tag="PKM87_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37ae"
                     /protein_id="HNQ29866.1"
                     /translation="MSRGQQKAKGRVTGSAGRFGPRYGRFIRKRVVETERISRAAHRC
                     PRCDTISVKRQGTGIWACRKCGFKFAGGAYTPQTPALRIALRAIDRTMEGKE"
     gene            complement(3609..4304)
                     /locus_tag="PKM87_07085"
     CDS             complement(3609..4304)
                     /locus_tag="PKM87_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly factor SBDS"
                     /protein_id="HNQ29867.1"
                     /translation="MIPLEKAVAARLESHGEKFEILVDPDLAVKSRQGEKIELEDMVA
                     ALNVFSNASRGTRASDEALLKVFHTTDFPEIAAKIIQKGEIHLTAEQRKHMIAEKRRQ
                     VINFISRNAINPQTGLPHPPQRIEMAMEEARVNIDLYKHLDELVKETVKALRPILPIK
                     FDELRIAVKIPADYAPRAFGDIQSAATIEREEWQKDGSWICLVRIPAGIQGDFYSLVN
                     RLTKGQGEVKILD"
     gene            complement(4318..5043)
                     /gene="psmA"
                     /locus_tag="PKM87_07090"
     CDS             complement(4318..5043)
                     /gene="psmA"
                     /locus_tag="PKM87_07090"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HNQ29868.1"
                     /translation="MQPQAYQMGYDRAITVFSPDGRLYQVEYAREAVKRGTTAVGIKC
                     REGVVLIVDKRISSRLLEASSIEKIFKIDEHIGVASSGLVGDARALVDRARIESQINR
                     VTYDEKIDVEALAKKLGDHMQTYTQFGGARPYGTALLIAGVSDGEGRLFETDPSGTLL
                     EYKATGIGIGRNAVMKVFEEEYDFDSTINDSIQLGIRALHDATEGKFDVNMVEIGIIE
                     MENPIFRKMTKEEVAAFMDKFEK"
     gene            complement(5050..5535)
                     /locus_tag="PKM87_07095"
     CDS             complement(5050..5535)
                     /locus_tag="PKM87_07095"
                     /inference="COORDINATES: protein motif:HMM:NF014013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpp14/Pop5 family protein"
                     /protein_id="HNQ29869.1"
                     /translation="MKPRPPTMRGKRRYILARLDPPSVAVSGRDLYYAIAESLTSLYG
                     DSGMARIQPSVVSCTGGYAIIRCIRSTEGQVSACLAAVHQVQEVSVAVRSIRISGTMR
                     SLRQHLAGMKPRVPCGEQDAYFQGRSWTGVQYPGAKVDLFEKGFKNQQPLFLTEEDLE
                     E"
     gene            complement(5532..6152)
                     /locus_tag="PKM87_07100"
     CDS             complement(5532..6152)
                     /locus_tag="PKM87_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P subunit p30 family protein"
                     /protein_id="HNQ29870.1"
                     /translation="MYPYPLGDSTVRRMAQEAGELGFDSMVAVGARGCTYKGVEVVGG
                     TIIQGRDQREVQAAMRRIDGNPGILMATAGDYTLNRALLHVGRVRVLQGIHATPKNSF
                     DHILSKMAAERQVAVDLDLSLLVSGRDYARQKALQRYADLAGLQSRFGFPFTLSSGAR
                     SILQMKSTRDMVALCGLFGLDEDGVGGALLGIAKILRPEKAVEVVE"
     gene            complement(6203..6793)
                     /locus_tag="PKM87_07105"
     CDS             complement(6203..6793)
                     /locus_tag="PKM87_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15e"
                     /protein_id="HNQ29871.1"
                     /translation="MAKSMYAYVREAWRRPSESEVKALLWNRMQQWRREGSVVRIERP
                     TRIDRARNLGYKAKQGIVMVRVKVRRGGRRKSRYVRARRSIRMGVNRITAGKSLQRIA
                     EERASRKYPNMEALNSYWVGQDGRQKWYEVILVDGNHPAIRTDSQLAWMSDHVHRGRA
                     ERGKTSAGMKGRGMGTRGKGTEKTRPSIRSHGNLGK"
     gene            complement(6922..7407)
                     /gene="moaC"
                     /locus_tag="PKM87_07110"
     CDS             complement(6922..7407)
                     /gene="moaC"
                     /locus_tag="PKM87_07110"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNQ29872.1"
                     /translation="MTEFTHIGDDRARMVDIGGKGEVYREARASGRILLRRETLSAIR
                     EGTVVKGNVLATARVAATLAVKETPRLIPMCHPIPISSVDVEFNILEDSVEAVIRVRS
                     HGRTGVEMEAITGVSVALLTVWDMVKSAEKDAGGQYPVTCITDIRVLEKRKGESGGSE
                     K"
     gene            complement(7404..8804)
                     /locus_tag="PKM87_07115"
     CDS             complement(7404..8804)
                     /locus_tag="PKM87_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNQ29873.1"
                     /translation="MVLRECREFQETGIITPSRMRAVDENAMALGVSSLQLMESAGKA
                     LADVVRSLKASPVVMLCGKGNNGGDGMVAARHLQDLDVTVVYLDGQGWSPDSGHQLKI
                     LSSCSVELLPVRCPEDVVSLGNHFQGAGCIVDALLGTGSTGNPREPIRSLVEAAQKTP
                     AVKVSADIPTPGFVPEHVLAFHRPKAEGSLVADIGIPLEAEITVGPGDLTILQRKDPG
                     AHKGAGGEVLVVGGGPYQGAPYLAGLGALRAGADIVRIASPVFEPVPDLIYERLEGER
                     IAARHLETLKTLAERADVVVMGNGLGDKSHGVMVELARYCKRAVVDADAIRRPLPQGE
                     DTIYTPHAGEFVRMTGVLPPEELVPRARAVRDAAHIGTILLKGRVDVISDGKRVRFNR
                     TGSPLMTVGGTGDVLSGVAAALFCHLPAFESACLAAYVNGRAGMAVEKAFGGGMLPTD
                     LVDRIPLELFRRNEPT"
     gene            8961..10358
                     /locus_tag="PKM87_07120"
     CDS             8961..10358
                     /locus_tag="PKM87_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNQ29874.1"
                     /translation="MSLCDDARSAAREILSHDEVTVISHIDADGIAGEAILAEALSRA
                     GIIHRSVFVRQLEPMTMRQVPEDTSFKLFCDLGAGQQNLLEERGLRDDEVLIVDHHVS
                     QPAGRAYQQVNCLDHGFSKMSAAGVAFIIAMEMDPGNVDLSRLAVVGNVGDMMAREDC
                     GLVGPARDIALQGASAGCIEVVSRDLNCYGTSTRPLYVCLAYNDDPFIPGITNNMNGA
                     LRFLQRLGIELQTPEKRWRVWEELSPQDKRLVTSSLVQQIISCGERADRLFSESYLFP
                     DEAARTPLRNAQEFATLLNACGRWTRAFVGSSICRGDRGGAYREAEHMLNNHRAVIRE
                     LLEYILTTGVRELSHIQWIHVGNRYPDTIVGIGAGMALSRLNMEKPILVMVEQPEDPG
                     LTKVSMRTTERAIGRGIDLQLALTTASAEVGGAGGGHRIAAGAFVPKESEEHFVDRVN
                     QLIAGQLCTEGQGNR"
     gene            10294..10857
                     /locus_tag="PKM87_07125"
     CDS             10294..10857
                     /locus_tag="PKM87_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNQ29875.1"
                     /translation="MSTGSTSSSQDSCVQRARVIADFQFGRGAGRGLFPDDCRFIFSR
                     TGRIRQVTWNGSRLATLRASDGRLTLGMAGAIRLHQALPAPAYRVRIVPEVAEFILKG
                     KNAFSKHVVEADPGIRSGDEVLVVTGDDELLATGAAVLSGREMLVFNYGVAVKVRQGR
                     SAYDAGKYQPQEDEADDEAAGDAGGAV"
     gene            10781..11125
                     /locus_tag="PKM87_07130"
     CDS             10781..11125
                     /locus_tag="PKM87_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nascent polypeptide-associated complex protein"
                     /protein_id="HNQ29876.1"
                     /translation="MMPGNINPRKMKQMMKQLGMQVEQFEDVRQIVITTGKGRYVFDA
                     AEVAAMTMQGITTYQITGEPRFEAAALEIPDDDVRMVAEQAQVSLDAARDALQKTGGD
                     IAEAIIHLTSHV"
     gene            11118..11843
                     /locus_tag="PKM87_07135"
     CDS             11118..11843
                     /locus_tag="PKM87_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNQ29877.1"
                     /translation="MFEAGERVLLVGGERTFFVRAGTGSFSTDRGVLDLDALLQVSQG
                     DVITTHTGQEFRALSPRPTDFFSRASRSGAPMLPKDIGLVIAYTGMNNRDTVLDAGTG
                     SGIAAIYFGGIAKAVRTCEIRPEFSRLAEKNIQDAKLPNVDVVCGDVLSEEGLYDVVH
                     FDLTLTPDHVHHAHSLLRPGGYLACYTPFIEHLFTVMDAAGELFTEIQAHECIEREMT
                     RSQRGSRPSTRVCHSGYVTVARK"
     gene            12247..13194
                     /locus_tag="PKM87_07140"
     CDS             12247..13194
                     /locus_tag="PKM87_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29878.1"
                     /translation="MAILTVGMAMAVDPVPATTETQGISTFTGAVFYGTMTNSESVVW
                     TESNMDVRDNPPLGQNPFGIPLSSFLWLNLNVNGQEIPILAPERQSVMSYTESVLADN
                     GYGEFNEIQALDTGNKVANQDNFKSTEQFDFVAFSDAMGRATTSESIMLDLASQGSDA
                     TKRFICPFATGDEGYIPAYCNVYEMGSSFSGSQVSMITQADENHIAKSADVPTQLAYS
                     IGLSGTGSTASWINAHIMEGRTGGHLEFGLGPVDIGPFNLGLDGNLLWNPDTGAPFTN
                     LGTGFMQSVDLTYKEKTTISGTIESFSKSMSVQDAVRRL"
     gene            13638..14615
                     /locus_tag="PKM87_07145"
     CDS             13638..14615
                     /locus_tag="PKM87_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29879.1"
                     /translation="MKKFAFAVLALAVLVVGTAMAVDPINATTETQGISTSTGVIVMG
                     TMTNSESVVWTESNMDVRDNPPLGQNPFGIPLSSFLWLNLNVNGQEIPILAPERQSVM
                     SYTESVLADNGYGEFNEIQALDTGNKVANQDNFKSTEQFDFVAFSDAMGRATTSESIM
                     LDLASQGSDATKRFICPFATGDEGYIPAYCNVYEMGSSFSGSQVSMITQADENHIAKS
                     ADVPTQLAYSIGLSGTGSTASWINAHIMEGRTGGHLEFGLGPVDIGPFNLGLDGNLLW
                     NPDTGAPFTNLGTGFMQSVDLTYKEKTTISGTIESFSKSMSVQDAVRRL"
     gene            15059..>15662
                     /locus_tag="PKM87_07150"
     CDS             15059..>15662
                     /locus_tag="PKM87_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29880.1"
                     /translation="MKKFAFAVLALAVLVVGTAMAVDPINATTETQGISTSTGVIVMG
                     TMTNSETVVMTMSNQDIRNNPPLNAWTAIDPVTGGPDLAAPWDNQWTPERQAVLSYTE
                     SILADNGYAEFNEMQSMSTANTVVNQDNFKSTEQYDYVAFSDAMGRTTNSESILLDLA
                     SQGSDAASRMICPFATGDNGYIPAYCNVYEMGSSFTGGQVS"
BASE COUNT         3284 a         4686 c         4305 g         3387 t
ORIGIN      
        1 tatcaagcgc ctcgttgctc tgccgccaca ccagcgaatc actctcagtg aacgttccga
       61 tgcagtcaac ggaagtaaga gtggagatcg actgggtttc cgcggtattc gggggcctga
      121 tatctgctga cactcctgga aaaagaataa tgaataccag aaccaaaagt atgctcaacc
      181 tgaaaaattt cattccttcc gtccccaatt agcaataact cgtaattttc gctaaagggt
      241 tatatatatg tcactattct gcaaaaaaac aggagaacgc ccaggtcgga attcgaatcc
      301 gagtcgtcgg cgtgacaggc cgacatgata ggccgctaca ctacctgggc acaccagtcc
      361 ttttcgcgca tctgattttg agatcccgcc tcccggattt gaaccgggga catcgcggta
      421 gccgcgcaat tgcctgaaac ggcagatcat actacagccg cgcactctac cagtctgagt
      481 taaggcggga tttgcgctca tactataggg ggaataaacc tattaaacgt atcgatttgg
      541 cgggaatggt attgcccggc cgcgtacttt tttatagtac ggacaacgta aaataaaggt
      601 atgacctcca gaaccactga tgtggggaat tgtcttcttt gtcgatagtc cgtcgcctag
      661 aagagtcact gttttcgata ccaccctcag ggatggtgaa cagacacccg gtatctcctt
      721 caaattcgag cagaaacttg agatagcccg ccagctctct tccatcgggg tccatgccat
      781 cgaggcaggg ttcccggcct catccaaagg agagatggag acggtgaagg caatcgcgga
      841 cctcgatctc gagtctgtgg tctgcggcct ggcccggtca aggaaagagg atgttgacgc
      901 ctgccttgac tgcggggtag acatggtgca tgtcttcatc cccacctccg aggtgcagcg
      961 ggtgcatacc atcaagaaga gctgcgaaga ggtgctcgag atcaccggcg agatcgtctc
     1021 ctacgtccgc gaacattgcg acctgtgcat gttctcggcg atggacgcga ccaggacaga
     1081 ccttgactac ctggtgcagg tctttcgcac cgccgcggat gcgggggcca caatcctcaa
     1141 cgtcccggac accgtgggag tctacactcc gaccgccatg aagggcctca tctcccatct
     1201 ggcagccgag atcaggtgcc cgctcgacgt gcactgtcat aacgacttcg gccttgcggt
     1261 cgccaacacc atcaccgccg tcgaggccgg cgcctcccag gtgcaggtga cggtgaacgg
     1321 gcttggtgag cgtgccggga acgcggacct ctcttcgacc gtgatgatcc tcgagtccat
     1381 cttcgggatg cagaccggga ttaaaaagga gaggctggtg gagacctcgc gtttgatctc
     1441 ccggtattcc gggatatcga tcccgcccac ctatccaatt gtcggggaga acgccttctc
     1501 acacgagagc gggatccact cccacggcgt gattgcctgt gccgccacgt ttgagcccgg
     1561 catcatgact cccgagatgg tgggccaccg ccggaagctg acactcggca agcacgtcgg
     1621 gaggcacgcg gtgcgccaga tgctcgaaga ggtccacctg gacccgagcg acagccagct
     1681 cgaccgtatc gtggagaaga tcaagttcat ctccgtgaaa ggaaagcggg tcacggacgc
     1741 cgacctctac gagatcgccg agagcgaaat ggggatcggg aacggcggcc ggatgatcga
     1801 gatggatgaa ctggcagtga tgaccggcaa ccacctcatt cccactgcga gcgtgaaggc
     1861 gaagatcggg ggaaaagaag cagtctacag tgccacgggg accgggccgg tggatgccgc
     1921 cctgaaagcc gtgctcggga tgctgccgat gaaggtgcag ctgaaggagt tctccatcga
     1981 ggccatctcc ggcgggtccg atgccatcgg ccatgtcacc atcgccgtcg aggacgagca
     2041 ggggaggatc ttcgatgcca gcgcatcggg ggacgacatc gtccttgcat cagctgaagc
     2101 gatgatcaat gccctgaaca tgctgcaccg gctggatccg cacaccggaa aaaagtgaat
     2161 gaaatgatcg tttaggcggc gccaggggat gatttcccct cgagcgcctg ctttatctgg
     2221 gtctggagct gctcgaactt cccctgcagc atcgtctcct gcttctcgag ggacttgatc
     2281 cggatctcca gggtctcgat cttgtcgttg agctttacca ggacgtcttc cttcttcttc
     2341 tgcatcatca cagagccaac gctcatgaag actgcattct cctccgggac ctctttcaac
     2401 tcctcggttg cacgcctggc ctccctgacc gccatctcgt actggttctt ctgggccgcg
     2461 atcgtctgca tctgctgctg catctgctgc agctgggcaa tctggttctg catttttggt
     2521 gatatactgc tcatgatctc tccttttgag caggcgatgc cagcgcctgc atctcgtccg
     2581 ccacgttcac gaggcggagc cacatgttca gtgctgcccg gagcgcggcg caatcccttg
     2641 cctgcacctt cagcaccagg acgccttgac cctccatgaa acactccccc cgggagcggg
     2701 ggtgcagctc agcagccatc tcggggctca gcgacctgaa cagtgtctct gcatggggac
     2761 accggaaatg aaagaccgct tcatgctcca ttcctcccat ctcaccctcg cacgccctct
     2821 cgttctccgg caagactggt ccccttcgtc tttttctgac ctttttattt ggacacggtc
     2881 ccacttaaac agattggtca catgcgaaaa acgcctctgt tcaaatagcg tgaccggcta
     2941 tgccactacc tcatacaggt cccattcata aaaatggttc gatgtattga ggaaagagaa
     3001 ctccacgaag tgaaaagatc aaataaaact acatgattct cttcaagaca caaacatacg
     3061 gatttatgaa aatgccgcag gcattttcat tcgaaaaaag gggagtagca ggaaaggcca
     3121 gtcagcgtgc tttcaattcc tttattgccg cgccccgctc cttgaagagg atgcggtggc
     3181 cgcagtaggg gcagcgcacg tttacgtcga tctcaacctt ctgcttgcac cgggcgcact
     3241 tgtacgctcc cgccataccg ggatcactcc tttccttcca tcgtgcggtc gattgcgcgg
     3301 agcgcgatgc ggagcgcggg tgtctgcggc gtatacgctc ctcctgcaaa tttgaacccg
     3361 cacttgcggc aggcccagat cccggtcccc tgcctcttta ccgagatagt gtcgcatctc
     3421 ggacagcggt gcgcagcacg ggagatgcgc tcggtctcga cgaccctctt gcggataaat
     3481 cttccatacc gtggcccgaa gcgccctgcg ctcccggtaa cccttccctt tgccttctgt
     3541 tgtcccctgc tcatatcttc atcccgttct cttgtgtctt ttattattca ctgtatgcct
     3601 tcatatagtt aatcgaggat cttcacttcg ccctggccct tggtaagccg gtttacaagg
     3661 ctgtagaaat ccccctggat ccctgcgggt atcctgacca ggcagatcca ggacccgtct
     3721 ttctgccact cctcgcgctc aatggtggcc gcggactgga tgtccccgaa tgcacggggc
     3781 gcataatctg caggtatctt caccgcgatc cggagctcgt caaactttat gggaaggatc
     3841 gggcgaagcg cctttaccgt ctccttcacg agttcgtcaa ggtgcttgta gagatcgatg
     3901 ttgaccctgg cctcttccat ggccatctcg atcctctgag gcgggtgcgg gagaccggtc
     3961 tgcggattga tggcgttccg ggagatgaaa ttgatcacct gccgccgctt ctcggcgatc
     4021 atatgtttcc gctgctccgc ggtgaggtgg atctcgccct tttggatgat cttcgccgca
     4081 atctccggaa aatccgtggt atggaacacc ttcaggagcg cctcgtccga tgccctggtc
     4141 ccccgcgagg cattcgagaa gacgttgagc gctgctacca tgtcttccag ttcgatcttc
     4201 tccccctgcc gagacttcac cgccaggtcc gggtccacca ggatctcgaa cttctcgcca
     4261 tgactctcca gtcgggctgc gaccgccttc tcaaggggta tcaccaacca ttccccctta
     4321 cttctcgaac ttgtccatga aggccgccac ttcttctttg gtcatcttcc ggaatatggg
     4381 gttctccatc tcgatgatgc cgatctccac catgttcacg tcgaatttgc cctcggtggc
     4441 gtcgtggagg gccctgattc ccagctgtat gctgtcgttg atggtcgagt caaagtcgta
     4501 ctcttcctcg aagaccttca tcaccgcatt cctgccgata cctatcccgg tcgccttgta
     4561 ttcgaggagg gtcccgctcg ggtcggtctc aaagaggcgg ccttctccgt cgctcacccc
     4621 tgcgatcagg agcgcggtcc cgtaaggcct cgcgccaccg aactgggtgt aggtctgcat
     4681 gtggtcgcca agtttcttcg caagggcctc cacgtcgatc ttttcgtcgt aggtgacgcg
     4741 gttgatctgg gactcgatcc gggcccggtc gacaagtgcc ctggcatccc cgacaaggcc
     4801 tgacgaggcg accccgatgt gctcatcgat cttgaagatc ttctcgatag acgaggcttc
     4861 caggaggcgg gagctaattc tcttgtcaac gatcagcacg acgccctccc tgcattttat
     4921 tcccaccgcc gtggtccctc tcttcactgc ttcccgggca tattcgacct gataaagccg
     4981 gccgtcgggg gaaaacactg tgattgcccg gtcatacccc atctgatatg cttgtggctg
     5041 catgattcgc tactcctcca gatcttcctc tgtgaggaac aatggttgct ggtttttaaa
     5101 tcccttttca aataaatcaa ccttcgcacc cggatactgc actccggtcc aggatctgcc
     5161 ctggaaatac gcatcctgct ctccgcatgg taccctgggt ttcatgcccg caaggtgctg
     5221 gcgcagggag cgcatggtcc ccgagatacg gatgctccgc accgccacgc tcacctcctg
     5281 cacctggtgc accgcggcga gacaggcgga tacctggcct tcagtggagc ggatacaccg
     5341 gataatggca tatccccctg tgcaggagac aaccgacggt tgaatcctgg ccatgcccga
     5401 atcgccgtag agcgaagtca gcgattctgc aatggcataa tagaggtcgc ggccgctgac
     5461 agcgacagat ggcgggtcga gccttgccag gatgtaccgc cgctttcccc tcatcgtggg
     5521 ggggcgcggt ttcattccac cacctcgact gccttttccg gacggagaat ctttgcgatg
     5581 ccgaggaggg cccctcccac cccatcttca tccaggccaa agagcccgca cagcgcgacc
     5641 atgtccctgg tggacttcat ctggaggatc gacctcgcac cggacgagag ggtgaacggg
     5701 aacccgaagc gggactggag accggccagg tcagcgtatc tctggagcgc cttctgcctg
     5761 gcataatccc tcccggaaac cagcaaagag aggtccaggt ccactgccac ctggcgttcc
     5821 gcggccattt ttgagaggat atggtcgaac gaatttttcg gagttgcatg gatcccctgt
     5881 aacaccctga ccctccctac gtgcaggagt gccctgttaa gcgtgtaatc ccccgcggtg
     5941 gccataagaa tcccaggatt cccgtcgatt ctccgcatcg cggcctgcac ctccctttga
     6001 tcccttccct ggatgatggt gccgcccacc acctccactc ccttataggt acagcccctt
     6061 gcgccaactg ccaccatgct gtcaaacccg agctccccag cctcttgtgc catgcggcgg
     6121 accgtggagt ccccgagggg ataggggtac acgcaggcgt cagtgcatcg catactaaaa
     6181 aagatcttgg gaagaggata agttatttac ccaggttacc atgagaacgg atgctcggcc
     6241 tggtcttctc ggtccctttc ccgcgggtcc ccatgccgcg gcccttcatc cccgcgctgg
     6301 tcttgccgcg ttctgcacgc ccccggtgga cgtggtcgct catccacgca agctgtgagt
     6361 cagtccggat cgccgggtgg ttcccgtcga ccaggatgac ctcgtaccat ttctggcggc
     6421 catcctgacc cacccagtag gagttcagcg cctccatgtt cgggtacttg cgcgatgcgc
     6481 gctcctcggc gatacgctgc aggctctttc cggcagtgat gcggttgact cccatcctga
     6541 tggacctgcg ggcccggacg tatcgggact tgcgcctgcc tccccggcgg accttcaccc
     6601 tcaccatcac gataccctgc tttgccttgt agccaaggtt ccttgcacgg tcgatacgcg
     6661 tggggcgctc tatccgtacc acgctcccct cgcggcgcca ctgctgcatg cggttccaga
     6721 gcagtgcctt gacctcgctc tcgctcggtc tcctccacgc ctccctgacg taagcgtaca
     6781 ttgattttgc catggttctt gcacctttca ggttcagctc gaaagacgag ccacattcct
     6841 tcacgggacg tatcccgtaa gacctataga agtcgcctgc agggatatta aactgcgcgg
     6901 tcagcgctac ccgtgcactt tttacttctc gctgccaccg ctctccccct tcctcttctc
     6961 gagcaccctg atatcggtga tgcaggtgac gggatactgg ccgcctgcgt ctttctccgc
     7021 ggacttcacc atgtcccaca cagtgaggag ggctactgat acccccgtga tggcctccat
     7081 ctcgacaccc gtcctcccat gcgaacggac tctgatcact gcctcaaccg agtcctccag
     7141 gatgttgaac tccacgtcca cgctgctgat gggaatggga tggcacatgg ggatcaggcg
     7201 gggagtctcc ttcactgcca gggtggcggc gaccctcgcg gtcgcaagta cgttgccctt
     7261 caccacggtc ccttccctga tggcagagag tgtctcccgc cggaggagga tcctccctga
     7321 tgcccgcgcc tcacggtata cttctccttt ccccccgatg tccaccatcc gggccctgtc
     7381 atctccaatg tgcgtaaatt ccgtcatgtg ggttcattcc tcctgaagag ttccagcggg
     7441 atacggtcga ccaggtctgt cgggagcatt cccccgccaa acgccttctc aaccgccatt
     7501 ccggctctcc cgttcacgta tgcagccagg cacgcggact cgaatgcggg caggtggcag
     7561 aagagggctg cggccacccc tgacaggacg tcccccgttc ctccgaccgt catcaggggc
     7621 gagcccgtcc ggttgaacct gacccgcttc ccgtccgaaa tgacatcgac cctccccttc
     7681 agcaggatgg taccgatgtg tgccgcatcc ctgactgctc ttgcccgggg aaccagttct
     7741 tcgggtggta acacgccggt catgcgaaca aattcgcctg catggggagt ataaatggta
     7801 tcctcaccct gtgggagcgg tcgcctgatc gcgtctgcat ccactacagc cctcttgcag
     7861 taccgggcaa gctccaccat cacgccatgg ctcttgtcgc cgaggccgtt tcccatcacc
     7921 accacgtcgg ccctttccgc gagagtcttg agggtttcaa ggtgccgggc ggcgatcctc
     7981 tccccttcca gccgctcgta gatgagatcc gggacaggct caaagacggg cgaagcgatc
     8041 ctcacgatgt cggctccggc ccggagcgcc ccgagcccgg caagatatgg tgctccctgg
     8101 taggggccgc cgccgacaac aagcacctcc cccccggccc ccttgtgcgc accggggtcc
     8161 ttccgttgca ggatagtgag gtcaccgggg cccacagtga tctcggcctc gagcggtatg
     8221 ccgatatccg caacgagcga tccctctgcc ttggggcggt ggaatgccag gacatgttcc
     8281 gggacgaaac cgggtgtggg gatatcggca gacactttca cggcaggcgt cttttgtgca
     8341 gcctccacaa gggacctgat cggctccctc ggattccctg tgctgccagt accaagcagc
     8401 gcatcgacga tgcaccccgc tccctggaaa tggttcccaa gtgagacgac gtcttcaggg
     8461 caccggacag gtagcaactc cactgagcac gacgaaagga tcttcaattg gtgcccgctg
     8521 tcagggctcc agccctgccc atccaggtat accacggtca catcgaggtc ctggagatgg
     8581 cgtgcggcga ccatgccgtc tccgccatta ttcccctttc cgcacagcat aacgaccggg
     8641 gatgccttga gggaccggac aacgtccgcc agggcctttc ccgcagattc catcaactgc
     8701 agtgaggaaa ctccaagcgc catggcgttc tcatcgaccg cccgcattct cgaaggggtg
     8761 atgattcccg tctcctggaa ctcccggcat tctcgcaaga ccatcagtca gatctcccgg
     8821 tacatgaagc gtcctgtacc aggatatcgt ccgttggaga tatgaagtat ccccctttgg
     8881 cgcgaaactt ccagatccgg cccaatttgc acccgtctca tccctacctt ttttctcctt
     8941 gatattccat tactatccca atgagtctct gcgacgacgc aaggtctgcg gcacgggaga
     9001 tcctctccca cgacgaagtg accgtcatat cccatatcga cgccgacggc atcgcagggg
     9061 aggctatcct cgcggaggcc ctctcgaggg ccggtatcat tcatcggtcg gtgtttgtca
     9121 ggcaactcga gcccatgacc atgcgccagg tgccggaaga tacctctttc aagctcttct
     9181 gcgaccttgg ggccggccag cagaacctcc tcgaggaaag ggggttgcgg gatgacgagg
     9241 tgctgatcgt ggaccaccat gtcagtcagc cggcagggag agcgtatcag caggtcaact
     9301 gtctcgacca tggtttttcc aagatgagtg ccgcgggggt ggcgttcatc atcgccatgg
     9361 agatggaccc gggcaatgtc gatctctccc ggctcgcagt ggtgggcaac gtgggggaca
     9421 tgatggcaag ggaggattgt ggccttgtgg ggccggccag ggatatcgcg ctacaagggg
     9481 cgtcagcagg gtgtatcgag gtcgtgtccc gcgacctgaa ctgctatggc acttccaccc
     9541 gcccgctcta cgtgtgcctc gcctacaacg acgatccctt catcccgggt atcacaaaca
     9601 acatgaacgg cgcgctccgg ttcctgcaga ggctcgggat tgaactccag acgcccgaaa
     9661 aacgctggcg ggtatgggag gagctgtctc cccaggataa gcgcctggtg accagttccc
     9721 tcgtccaaca gatcatatcc tgtggagagc gggccgaccg gcttttctcc gagagctacc
     9781 tctttcccga tgaggctgca aggacgccgc ttcggaatgc gcaggagttt gccacgctct
     9841 tgaatgcctg cgggaggtgg acccgcgctt ttgtgggaag ttccatctgc aggggggatc
     9901 ggggcggtgc atacagggaa gcagagcaca tgctgaacaa ccaccgggcg gtgatcaggg
     9961 aactcctcga gtacatcctc actacagggg tccgggaact ctctcatata cagtggattc
    10021 acgtgggcaa ccggtatccc gacaccattg taggtatcgg cgccgggatg gcgctctcac
    10081 gcctgaacat ggagaagcct atcctcgtga tggtagagca gcccgaagat cccgggctca
    10141 ccaaggtctc catgcggacc acggagaggg cgataggacg gggaatagac ctgcagcttg
    10201 cgctcaccac cgcgtctgcc gaggtaggcg gagccggggg aggacatcgc attgcagcgg
    10261 gagcattcgt accaaaggaa tcggaggagc actttgtcga cagggtcaac cagctcatcg
    10321 caggacagtt gtgtacagag ggccagggta atcgctgact tccagttcgg gcggggggcg
    10381 gggagggggc ttttccccga tgactgcagg ttcatcttct cgaggaccgg tcggatccgc
    10441 caggtgacct ggaacggctc gcgccttgcc accctgcggg cctctgacgg gaggctcacg
    10501 ctcggcatgg ccggcgctat ccggctgcac caggccctcc ccgcgccggc ctaccgggta
    10561 cgcatagtcc cggaagtcgc cgagttcatc ctgaagggaa agaacgcgtt ctccaagcac
    10621 gtggtcgagg ccgaccctgg catccggagc ggggacgagg tgctcgtggt cacgggagat
    10681 gatgaactgc tcgcgaccgg cgctgccgtc ctgtcgggcc gggagatgct ggtatttaat
    10741 tacggtgtag cggtaaaagt acgtcaggga aggtcagcat atgatgccgg gaaatatcaa
    10801 ccccaggaag atgaagcaga tgatgaagca gctggggatg caggtggagc agtttgaaga
    10861 cgtgcggcag atcgtgatca ccaccgggaa gggaaggtac gtctttgatg ccgcagaagt
    10921 cgcggcaatg accatgcagg ggatcaccac ctaccagatc accggggagc cgaggttcga
    10981 ggccgccgct ttggaaatcc cggatgatga cgtcaggatg gtggccgagc aggcccaggt
    11041 cagcctcgat gccgcccgcg atgccctcca aaagacgggc ggggatatcg ccgaggccat
    11101 catccatctc accagccatg tttgaggcag gggaacgtgt ccttttagtc ggaggagaga
    11161 ggacattttt tgtccgggcc gggacagggt cgttctccac cgacaggggt gtcctcgacc
    11221 tcgatgccct gctgcaggta tcgcagggtg atgtcatcac tacccacacg gggcaggaat
    11281 tccgtgcact ttctccccgc ccgactgact tcttctcgcg ggccagccgg agcggtgctc
    11341 ccatgctccc gaaggacatc ggtcttgtca tcgcttacac gggaatgaat aacagggata
    11401 ctgtgctcga tgccgggacc gggagcggca ttgccgccat ttatttcggt ggcatcgcaa
    11461 aggctgtcag gacctgcgaa atacggcccg aattctcccg cctggccgag aagaacatcc
    11521 aggacgcgaa gctcccgaat gtggatgtgg tctgcgggga tgtcttgagt gaggaggggc
    11581 tttacgacgt cgttcacttc gatctcaccc ttactcccga tcacgtgcac cacgcccatt
    11641 cgcttctccg ccccggaggc taccttgcct gctatactcc gttcatcgag cacctcttca
    11701 ccgtgatgga tgccgcagga gagttattca ccgaaattca ggcacacgaa tgcatcgagc
    11761 gggagatgac acgaagtcaa cgaggttcgc gcccttctac ccgcgtgtgc catagcgggt
    11821 atgtcacggt tgcgagaaag taaagaccag gtggagatac ccctatggag tatatgaatc
    11881 gcctggtttt ctggtccagt gtaaaaaaaa tacggagcat tgaggctgct tttcagccgc
    11941 cgctctcctg acatttatcc tgattccaat cgtcaatccg gaacaagaac aggtcagcct
    12001 tctgaatcag cgccaatatg atagaaggca ggactgcagg gcagtgggaa caggatggaa
    12061 ccagcttaaa atattctatt cgtaggccgt ggagaagcct atcctgctcc gttttatacg
    12121 gatttggggt gcaagagata aaacgaattt tttttattct gaaagtggaa acgcatttct
    12181 atgctgtttt ttcagcatgc gaaatgagtg gtgtgtatga gaaaaattat atttatcttc
    12241 atatccttgg ccatcctcac cgtaggaatg gctatggcgg tggatcctgt ccccgcaact
    12301 accgagacac agggcatatc gacttttact ggagcagtat tttatggaac catgaccaat
    12361 tcggagtcgg ttgtctggac cgaatccaac atggatgtcc gtgacaatcc gcccctcggg
    12421 cagaatccat tcgggattcc cctgagcagt ttcctatggc ttaacctgaa tgtgaatggc
    12481 caggagattc cgatattagc tcccgagcgc cagtctgtca tgtcgtatac cgaatccgtc
    12541 ctcgctgaca acggttacgg cgagttcaac gagatccagg cgctggacac cggcaacaag
    12601 gtcgcaaacc aggacaattt caagtcaacc gaacagttcg acttcgtggc attctccgac
    12661 gccatgggca gggcaaccac gtccgagagc ataatgctcg acctggcaag ccagggatcc
    12721 gatgcaacaa agaggttcat ctgtccattt gcgaccggtg atgagggata catcccggcg
    12781 tactgcaacg tctatgagat gggcagctcc tttagcggga gccaggtatc gatgatcacg
    12841 caggctgatg agaaccatat cgcaaagtct gctgacgtcc cgacgcaact ggcgtattcc
    12901 attggcctct cagggaccgg ttccactgcc tcctggatca atgcgcacat catggaaggc
    12961 cgtactggtg gacatctcga gttcggactc gggccagttg acattggtcc atttaatctg
    13021 ggtcttgatg ggaatctcct ctggaacccc gacactggtg ctcccttcac aaatcttgga
    13081 acaggcttca tgcagagcgt tgacctgacc tacaaggaga aaactaccat atccggtaca
    13141 atcgagtcct tcagcaagtc catgagtgtc caggacgcgg tccggcggct gtaacattcc
    13201 agaattgaaa attgaatgca ggggcatctt gaaagccccg atttttttgt ttcccgcgag
    13261 gggtgaatag gcccatgatt cctgtatctg aaacctgcgg gggatttcca tggatgtccc
    13321 gtgcacgcgc ctgtcagctt gcccatgcat acgagattcc gtatgtggat cggaaaatac
    13381 acgggaatat tgtgatgaat agcaatatag ccccttttat gcagaaacca atttccaggc
    13441 catgacctta ctcatcgttg cacccatggc agccaaccaa tatgaacccg aacttatcaa
    13501 ttcatgaccg cttctgaaaa agaaaagccc agaatctccc atctttgaac agaaaggaga
    13561 gaaaggttct tatgctgctg tagctaaaat gtctggttga tgctgagagt atcagcataa
    13621 actgtaggag gaacaagatg aagaaattcg cattcgcggt cctcgccctc gccgtcctcg
    13681 ttgtgggaac ggcaatggca gtagacccca tcaatgcaac caccgagacc cagggcatct
    13741 ccacttcgac gggagtcatt gtcatgggaa ccatgaccaa ttcggagtcg gttgtctgga
    13801 ccgaatccaa catggatgtc cgtgacaatc cgcccctcgg gcagaatcca ttcgggattc
    13861 ccctgagcag tttcctatgg cttaacctga atgtgaatgg ccaggagatt ccgatattag
    13921 ctcccgagcg ccagtctgtc atgtcgtata ccgaatccgt cctcgctgac aacggttacg
    13981 gcgagttcaa cgagatccag gcgctggaca ccggcaacaa ggtcgcaaac caggacaatt
    14041 tcaagtcaac cgaacagttc gacttcgtgg cattctccga cgccatgggc agggcaacca
    14101 cgtccgagag cataatgctc gacctggcaa gccagggatc cgatgcaaca aagaggttca
    14161 tctgtccatt tgcgaccggt gatgagggat acatcccggc gtactgcaac gtctatgaga
    14221 tgggcagctc ctttagcggg agccaggtat cgatgatcac gcaggctgat gagaaccata
    14281 tcgcaaagtc tgctgacgtc ccgacgcaac tggcgtattc cattggcctc tcagggaccg
    14341 gttccactgc ctcctggatc aatgcgcaca tcatggaagg ccgtactggt ggacatctcg
    14401 agttcggact cgggccagtt gacattggtc catttaatct gggtcttgat gggaatctcc
    14461 tctggaaccc cgacactggt gctcccttca caaatcttgg aacaggcttc atgcagagcg
    14521 ttgacctgac ctacaaggag aaaactacca tatccggtac aatcgagtcc ttcagcaagt
    14581 ccatgagtgt ccaggacgcg gtccggcggc tgtaacattc cagaattgaa aattgaatgc
    14641 aggggcatct tgaaagcccc gatttttttg tttcccgcga ggggtgaata ggcccatgat
    14701 tcctgtatct gaaacctgcg ggggatttcc atggatgtcc cgtgcacgcg cctgtcagct
    14761 tgcccatgca tacgagattc cgtatgtgga tcggaaaata cacgggaata ttgtgatgaa
    14821 tagcaatata gcccctttta tgcagaaacc aatttccagg ccatgacctt actcatcgtt
    14881 gcacccatgg cagccaacca atatgaaccc gaacttatca attcatgacc gcttctgaaa
    14941 aagaaaagcc cagaatctcc catctttgaa cagaaaggag agaaaggttc ttatgctgct
    15001 gtagctaaaa tgtctggttg atgctgagag tatcagcata aactgtagga ggaacaagat
    15061 gaagaaattc gcattcgcgg tcctcgccct cgccgtcctc gttgtgggaa cggcaatggc
    15121 agtagacccc atcaatgcaa ccaccgagac ccagggcatc tccacttcga cgggagtcat
    15181 tgtcatggga accatgacca actcggagac cgttgttatg accatgtcca accaggacat
    15241 ccggaataac ccgccgctga acgcgtggac ggcaattgac cccgttactg gagggcctga
    15301 tctcgcagca ccctgggaca accagtggac ccccgagcgc caggctgtgc tgtcctacac
    15361 cgagtctatc ctcgctgaca atgggtacgc cgagttcaac gagatgcagt ccatgagcac
    15421 tgcaaacacg gtagtcaacc aggacaactt caagtcgact gaacagtacg actacgttgc
    15481 cttcagcgac gccatgggaa ggaccaccaa ctctgagagc atcctgctcg accttgcaag
    15541 ccagggatcc gacgcagcca gccggatgat ctgtccgttc gcgaccggcg acaacggtta
    15601 catcccggca tactgcaacg tctacgagat gggcagctcc ttcaccggtg gccaggtctc
    15661 ga
//
LOCUS       DAOQYR010000050        15418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_4294_length_15418_cov_8.814815, whole genome shotgun sequence.
ACCESSION   DAOQYR010000050 DAOQYR010000000
VERSION     DAOQYR010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 15418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15418
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4294_length_15418_cov_8.814815"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(691..2193)
                     /locus_tag="PKM87_07155"
     CDS             complement(691..2193)
                     /locus_tag="PKM87_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNQ29881.1"
                     /translation="MIDPLDVAIVALYFCGLVGIGILASRKIHSMSDYVVAGRSLGFW
                     LFTMLMVGSVCSGMSLLGVSGLGFSYGWPTIWEQIFVPLSVGFCIVFFGVKIHGLAKK
                     NGYLTVQDYFVHRYESPKALRSLSALSGIVVSMIYLAGQYTAVSIVLMWIFGLPHWVA
                     LLLGAFIVTIYTVIGGLYAVTWTTLLQGLILIIGVVIMAPMVIMKAGGFTHINEVMAA
                     TSPDLVQPWVGSGLFTPAYVVSFSLLLMIGLACSPHVINNILAVKDSRYFGWAPLIAF
                     LVYGAVMFLLKFGGFAGIVLVQEGIVTLPDLKNAGDFVFIYGIQSAIPHIVVWGLFAV
                     VILAAVMSTTDRLMLTIGTYFSWDIYRNVFRPGASDAQVLLVSKIAVAATAIITVLLA
                     LDPPAMLVWLIWAGIGIMFATFAVPLLAGLYWRRATREGALAAMALGLVSSCASGGIT
                     YFKIAVLPVHFSLYGVAVATFAMVAVSLATRPVDSRILDDTYTGWYIRVK"
     gene            2610..3221
                     /locus_tag="PKM87_07160"
     CDS             2610..3221
                     /locus_tag="PKM87_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29882.1"
                     /translation="MAEDIRDLIDKIREDGIRAAEEKAREIETRAHESADEIRASARL
                     EAEKTLAAAQERIKREEERERALLAQAGRDLLLSLRSEINAMLGRIILSDVQDVLTPE
                     SLFGLLHATITHYSGEETGRIEVEVKREDLEALEKSYLSRLREATKKNIVLRPSDEIS
                     GGFIISFDNGRSCFDFSDKSLAEYIGTHLKPKLHAILEESTRD"
     gene            3267..3767
                     /locus_tag="PKM87_07165"
     CDS             3267..3767
                     /locus_tag="PKM87_07165"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HNQ29883.1"
                     /translation="MLHFGMKPPFSFAGFLETCREFIPERDYQVLCSLGEPAGCGEDR
                     ATPKIIRGWMEFDGALRNELVKARAQRLHVEPSQYLRKDVSPDQSLAAAVTAAVGNPS
                     PLDAERSLDEVRWKALDELATGHHFDLDFLITYAWKLLILIRWEHVRNADPVGILEHT
                     LQKVRG"
     gene            3768..5504
                     /locus_tag="PKM87_07170"
     CDS             3768..5504
                     /locus_tag="PKM87_07170"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNQ29884.1"
                     /translation="MTTSRKGRITRISGNMVTASFDSPVMQNEVAYILHGEERLKSEI
                     IRVRGNLAEMQVYEDTSRIRVGEEVEFTGDLLSVELGPGLLGQIFDGLQNPLPELAAQ
                     CGFFLRRGVYMEALSGSDAWEFTPVAQTGDRLRAGDRIGFVQEKIFRHYCMVPFSWEG
                     FFEVISVEKAGKYTKNQAIAHVKDEKGNVRPVTLCHRWPVKIPIRAYAEKLKPTEPLV
                     TKMRLIDSIFPVARGGTYCIPGPFGAGKTVLQQLISRHADADVVVIAACGERAGEVVE
                     TIKTFPTLIDPRTSRPLSDRTVIICNTSSMPVAARESSVYTAVTIAEYYRQMGLHVLL
                     LADSTSRWAQAMREMSGRLEEIPGEEAFPAYLESHIASFYERAGLVRLHDGSLGSVTI
                     GGAVSPAGGNFEEPVTQATLKVVGAFHGLSRSRSDARRYPAIDPLISWSKYGSFIDRE
                     QRETGRWLLRKGNDVAQMMKVVGEEGTSLADYTVYLKSEFFDFVYLQQNAFDPVDEAT
                     QKERQIYIFDFIYQIIKSDFSFKNKDEALHFFQQLRQLFRGWNSAPWQSEEFERTEQE
                     IRGLLSERMKTG"
     gene            5506..6810
                     /locus_tag="PKM87_07175"
     CDS             5506..6810
                     /locus_tag="PKM87_07175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF002555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNQ29885.1"
                     /translation="MQKFYSNITQIVGDVITVEAENVGNMELAQVTTRRGTSLAQAIR
                     LDGKKVFLQVFAGSRGISTGDKVRFLGHTMQIPTGDALLGRIFNGSGSPLDRGPALDE
                     NLVEVGGPPVNPVKRIIPDKMIRTGIPMIDVFNSLVESQKLPIFSIAGEPYNELLARI
                     AIQAEVDIIILGGMGLKYDDFIFFRETLGEHGALSRSIVFMHTAADPIVECLLVPDIS
                     LAVAENFALAGKRVLVLLTDMSNFCDALKVISITMEQIPSNRGYPGDLYSQLAARYEK
                     AVDFDTAGSITILAATTMPGDDVTHPIPDNTGYITEGQFYLKNSVIEPFGSLSRLKQQ
                     VNGKTRDDHRTIMDTMIQLFAEYRETLEKQAMGFEMSAWDQKLLKYGRLFEEKMMSLG
                     VNIPLERALDLGWEILHECFLPEETGIKRSMIDAYWPGTHEG"
     gene            6800..7411
                     /locus_tag="PKM87_07180"
     CDS             6800..7411
                     /locus_tag="PKM87_07180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNQ29886.1"
                     /translation="MKVKLSQGELKRQRDALRQYERYLPTLQLKKQQIQLEILSQQGA
                     MRKARAALSRKMEAASEWSGLLTEDPGIARWLLPEQVVTARKNVAGVDLPVFERAEFE
                     TAEYDLFLAPLWLDRGLEVLREIVSLQEEIRVLETGISILHHDLRITTQRVNLFEKVK
                     IPEAKEAIRLIKIHLGDQMTNAVGRSKIAKKKIEASNASEAGP"
     gene            7408..9195
                     /locus_tag="PKM87_07185"
     CDS             7408..9195
                     /locus_tag="PKM87_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29887.1"
                     /translation="MIVPMKKASILCEARDADSTVKTLRRLGVMHVEHQNTPGNVDIT
                     GLEEQVALIDSTLDVLDQVAASEKEIPNEETLLDADWKAVADRVIELGRRNDQTEILS
                     RGIIAQINEWERWGDIDAAEIRELSEKGIHLSLCQVPRKEIKNFPDDVVVRTIFTTGD
                     IANCAVISRRAFECPFPEITPPKQGVSALRRQLQDMTAGIELARRELLFCARYRNSLS
                     AIRIALEREIEFHQVLSGMGREGEINYVTGYIPNDAEEQLIEAAREKKWGVMITVPSD
                     IDEVPTLLRNPKWVSLIKPVLDLLGLTPGYRELDISLLFLVFFSIFFGILIGDAGYGV
                     VYILITLVLQKVVLKSWVLDHPEMKTTFALFYLLGACLVIWGLLTGVFFGQSWLLELG
                     YTPVVPQLNDPQFMQTFCFFLGALHLSIAHLWRVILKFPSLRLLADIGWICVLWTAFF
                     LARTLILGDPFPLLGIWLIIAGIVLVILFTHPQRNVLKGIATGLGTIALSLVNNFTDV
                     ISYIRLFAVGLATLAIAETTNSLAFGFGDGIVALVVGVVILITGHGLNIILGPMSVLV
                     HGVRLNVLEFSGHANVIWSGTAYEPFKEE"
     gene            9211..9666
                     /locus_tag="PKM87_07190"
     CDS             9211..9666
                     /locus_tag="PKM87_07190"
                     /inference="COORDINATES: protein motif:HMM:NF005174.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29888.1"
                     /translation="MDANLLTQFQDLGMAMVLALSACGSAAGTGTAGMAAIGAWKKNY
                     SQNKPAAFMLVAFVGAPLTQTIYGMIVMNQMSQLAMQGVFLWGIGGFCGAAMGLSAYF
                     QGKCGACACDSMGETGKGFGNYMIVLGMVETVALLVMAFTLGILGSFGT"
     gene            complement(9906..10775)
                     /locus_tag="PKM87_07195"
     CDS             complement(9906..10775)
                     /locus_tag="PKM87_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNQ29889.1"
                     /translation="MFSKILFPTDFSEYAAKTLDCIAGFPGVREVVLLHVVKETRSPR
                     GGGEIGKALFHDGRITLREEKHHLESLGENIRVQTVVKVASDIAGAIVQAAEENGVSL
                     VVIGARGSSLVEGILLGSVSKAVVRRSRTSVLIMRHKITQGLDGKTYEMFCPMILSRV
                     LCPVDFSSYSDDAIARLHATPGVGEAILLCVVSRGETKEEIEDNQRRAREKIEIIRKQ
                     FAAQGIRARAIVRLGNPAFEITKVAEEEDVSVIWMSSVGRGWFAELLIGSTAGAVAVN
                     SRRPVIIVRSKDT"
     gene            11381..11557
                     /locus_tag="PKM87_07200"
     CDS             11381..11557
                     /locus_tag="PKM87_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29890.1"
                     /translation="MVNEKALQTIEKMKLLVLKRCEERVQNASVSSHYTPPERFPYPQ
                     CHGVTAILRHRHGL"
     gene            11815..12135
                     /locus_tag="PKM87_07205"
     CDS             11815..12135
                     /locus_tag="PKM87_07205"
                     /inference="COORDINATES: protein motif:HMM:NF028309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit subunit G family protein"
                     /protein_id="HNQ29891.1"
                     /translation="MATEKSLLTKIREKELEVSVKIDDARMEADRMIERARKESQLIL
                     GASETEGKKAAGDFLRKEMERIQAEADQIRVQATREVESVRKKGEHRIPDAVEKIIAI
                     ILPG"
     gene            12142..14094
                     /locus_tag="PKM87_07210"
     CDS             12142..14094
                     /locus_tag="PKM87_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HNQ29892.1"
                     /translation="MLQKMIKIQVLGPKKDLHNVLDVLYAKGTVHIEDASGPSSPGEV
                     TLKRMTAEQVGDINVAYSKIGGILLTLPKTKIDEKAVAHTYANLKAMSHDNLLIHVNS
                     VIDDLEVATKTLAKEKSDLELSLINLGRYEKIIDKIRPLETSMPKLEGFEVTVLLIQK
                     EFKDVIDIIRDALAEITKRQFELLSAELDETTIAAVTIFNKRFSDQVHSYFFNQNVNE
                     VRLPPEYLGKSFNEIFSLIEEKKISSLKEIARLDSELEKISSKWYTELNALHRILGER
                     AEELSIFSKIGQTEYTFMLAGWIPKKSLRETREALMEGFGGRVIVNEPALTPEEMAEA
                     PTFYDNPRIVKPFEFLMCVVSPAKSTEYDPSPLMAIFLPLFFGIMVGDIGYGLIIAAF
                     ALIMKWKFSDRDWLQHLMNVLILGAIWTIIFGWLYGEFLGDLGEHMGWIEPKTFFGVT
                     WNRMEAIVPFLILSFAIGVFHVFLGLVLGIVNAWTEMSCKRHVDKAKKHICEKSGMII
                     VITGLILVILGIALALPSSIVTTGAVLIIVAIPLIFYGGGAFGTFEIMSTVTNILSYS
                     RIMAIGMASVILAVVANKLGGMMEVALVGLIIAALLHTLNLILAMFSPFLHSLRLHLV
                     EFDSKFYQGGGKMYKPFKKENGGKST"
     gene            14302..14520
                     /locus_tag="PKM87_07215"
     CDS             14302..14520
                     /locus_tag="PKM87_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNQ29893.1"
                     /translation="MVDFGIPIGAGIAFGLGALGTGIAQSRIGAAGAGTIAEKPEMFG
                     LMIILVAIPETLVILGFVVASMIMIMLV"
     gene            14589..15173
                     /locus_tag="PKM87_07220"
     CDS             14589..15173
                     /locus_tag="PKM87_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HNQ29894.1"
                     /translation="MAIDDLIKAVEVSGQERIKEIQERSKAEADEIIREAHVKDLPIK
                     KRFMDGAIQSVAVQRNKILSEAREKSRMEIIQSKNEVFERVFEEAVQKLASIRKDPGY
                     RAILKHLLLESIGQLGSDSVILHVDRKDEDLLKDILKEINVSGQIVPDLTCAGGLNAY
                     SRDERFVIFNTLESRLKKAKEIYRPEMFSVLFGE"
     gene            15175..>15418
                     /locus_tag="PKM87_07225"
     CDS             15175..>15418
                     /locus_tag="PKM87_07225"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HNQ29895.1"
                     /translation="MEYAYVNARIKAMKSRLLDRSFLEQLINKPDIDSLIAELEKTTY
                     REELDKAGVLYTGIHRIEVAIRKDLVKTFRTVLNLIR"
BASE COUNT         3824 a         4045 c         4223 g         3326 t
ORIGIN      
        1 atggtggaaa ccctgccctg aaaggcaggg tggacgcctc ctgttgatgc caccatcatt
       61 ccttttgcat atcactccag tggaatccac gatccattgt gcgtcactgc ctgcatagaa
      121 ctggctgaga gaaaagcaat ggaaaaaatg ccggtattct tatggtagga gatgccccgt
      181 gaggattctt tcggacacac atgttcggcc ccaggatgcc gcgtctctct cacccgcatc
      241 tcttccgccg ttttgggatg caggttcggg gcgtcccggc atcatgttcc ttgaacatac
      301 ctcttcggcc cccgtgatgg aatgtctctg ccgcccatgg cctttttcgc cgctctggac
      361 catgaactca ttccatactg gtagtatgtt tcctgaacat ttgttcggcc ccaggatgcg
      421 atgtcctgat cgctcccgta tcgttcagcc gtttggatac gggaactcat ttcatcatgg
      481 catgtatccc cagtcggttc ctcactgagg cattccccat gtagtcacaa tagtataaat
      541 agtttttctt tttgtacgtt gaatatcttt ttataaagta tataaatgtc gggaccgcgc
      601 gcggtaacgt ggcttccaaa agtatcactc ttgcccttcc aatccccctg ccatgaatca
      661 aagcgcccag cccctgcaga agaaagggta ttatttcacc cggatatacc accccgtata
      721 cgtgtcatca aggatacggc tgtccacggg cctcgttgca aggctgaccg caaccatggc
      781 aaaagtggcc accgcaaccc catagaggct gaagtgaacc gggagcaccg cgatcttgaa
      841 atacgtgatc ccaccagacg cgcatgacga cacgaggccg agagccatgg ccgcaagagc
      901 cccttcgcgc gtcgctctcc gccagtacag gccggcgagg agtggcactg caaacgtagc
      961 aaacatgatg ccaatcccgg cccagatgag ccagaccagc atcgcaggcg ggtcaagcgc
     1021 gagcagtacc gtgatgattg cggtagccgc aaccgcgatc ttgctgacaa ggagcacctg
     1081 cgcatcggag gctcccggtc gaaagacgtt ccggtaaata tcccaactga agtatgtgcc
     1141 gatggtcagc atcagcctgt ctgttgtgga catcacggct gccaggatca ccaccgcaaa
     1201 caggccccac acgacgatat gggggatggc cgactggata ccgtagatga agacgaaatc
     1261 accggcattc ttcaaatcag gaagagtgac tatcccctcc tggaccagca cgatgccggc
     1321 aaagcctcca aatttcaaaa ggaacatgac cgccccgtat acaagaaaag cgatcagggg
     1381 agcccacccg aagtaccggg agtccttcac tgccaggata ttgttgatca cgtggggaga
     1441 acatgccagc cctatcatca ggaggagtga gaacgacacc acgtatgccg gggtgaagag
     1501 tccactcccg acccatggct gcaccagatc aggactggtt gccgccatca cctcgttgat
     1561 atgggtaaac cctcctgcct tcatgattac catcggtgcc ataatcacca ctccgataat
     1621 gaggatgagc ccctggagaa gagtggtcca ggtgacagcg tagaggcccc cgatgaccgt
     1681 gtagattgtc acaatgaacg cgccaagaag cagtgctacc cagtgtggga ggccgaagat
     1741 ccacatcagc acaatgctga ctgcagtata ctgacccgca agatagatca tcgagactac
     1801 gatgccggaa agggccgaga gtgaccgcaa cgctttcggg ctttcataac ggtgaacaaa
     1861 gtaatcctgc accgtgaggt agccattctt ctttgcaagc ccatggatct tcaccccaaa
     1921 gaagacgatg cagaacccaa cagagagtgg gacaaagatc tgctcccata tggtcggcca
     1981 accgtaggaa aatccaagcc ctgatacgcc aaggagcgac attccgctgc agaccgaacc
     2041 caccatcagc attgtgaaga gccagaatcc aagcgacctg cccgcgacca cgtaatcgct
     2101 catggaatga atcttcctgg aagccaggat cccaatccct accaggccgc agaaataaag
     2161 cgcgactatc gcgacatcca aaggatcgat cacgggagag acccccggta atggagaccc
     2221 cataagaaga gcaggattat caccagcaat actgtgccgc ctacggtggc aatggtgatc
     2281 aagggcagtc cgaacatgag tggatcatca ttccatgcac gataaaatga cataatggat
     2341 ccgataattt tcggaaaaac ccaggatacc ggattcctga tagattcccc caattttttg
     2401 gttcgccttc aaacccttct ccgtatataa ggcaattggc tggctccgcc attcgctgaa
     2461 atccccgttc caaacattta tatggactat ttttttatac ccactcattc ccacacaaat
     2521 gatccggcat gcaagatacg cgaaagaatc gagttgatta tattgctgta tcccatccta
     2581 atatgcagtg acagggaaga gggcgcccaa tggcagagga tatcagggac ctgatcgata
     2641 aaatccgcga ggacggcatc agggctgcag aggaaaaagc ccgtgagatc gaaacccgtg
     2701 cacatgagag cgcggacgag atacgcgctt cggcaaggct cgaggcagaa aaaacccttg
     2761 ctgctgcaca ggagaggata aaacgcgagg aggagaggga gagagccctt ctcgcccagg
     2821 caggaagaga cctgctcctc tccctccgga gcgaaataaa tgccatgctc gggcggatta
     2881 ttctttctga tgtgcaggat gtactcaccc ccgaatccct ctttgggctg ctccatgcaa
     2941 ctatcacgca ctacagtggc gaggagacag gtcgcataga ggtagaagtg aagagagaag
     3001 acttagaagc ccttgaaaag tcttaccttt cgcgtctgcg ggaagcgaca aagaaaaaca
     3061 tcgtcctccg gccatctgac gagatctcag gggggttcat tatcagtttc gacaacggca
     3121 ggtcatgctt cgatttctct gataaaagcc ttgcagagta tatcgggacg caccttaagc
     3181 ccaaattgca cgcgattctc gaggaatcga cccgggattg aggcaaaatg gctgattttt
     3241 atccttacct ggtttcgagt ctccccatgc tgcatttcgg catgaaaccc ccgttctctt
     3301 ttgcagggtt cctcgagaca tgccgcgagt tcatcccgga acgcgattac caggtgttat
     3361 gcagcctcgg ggagcctgct ggttgcgggg aggatcgcgc aaccccgaag attatcaggg
     3421 gttggatgga gtttgatggt gcgctcagaa acgagctggt gaaggcccgg gcccagagat
     3481 tgcatgtcga accatcgcaa tacttgcgca aggatgtctc ccccgatcag tctcttgctg
     3541 cagcggtgac ggcggcggtg ggcaaccctt ctcctctcga tgctgagagg tcccttgacg
     3601 aggtgcgctg gaaggccctt gatgaactcg ccaccggcca ccattttgat ttggatttcc
     3661 tgataaccta tgcctggaaa ttactgatac tcatcaggtg ggagcatgtg cgcaatgcgg
     3721 atccggtcgg aattcttgaa catacactgc agaaggtacg gggatagatg accacgtcga
     3781 gaaagggccg gataaccagg ataagcggca acatggtgac ggccagtttc gattccccgg
     3841 tcatgcagaa cgaggtcgcc tacatcctcc acggggaaga gcgtctcaag tctgagatca
     3901 tccgcgtgcg cggaaatctt gcagagatgc aggtctacga ggataccagc cgcatcaggg
     3961 taggtgagga ggtggagttc acaggagacc ttctctcggt ggaactaggc cccggattgc
     4021 tcgggcagat ctttgatggc ctgcagaacc ccctgccaga gcttgcagca caatgcggtt
     4081 tcttcctgag gcgcggagtc tacatggagg cgctttcggg atctgatgca tgggaattta
     4141 ccccggtcgc gcagacgggg gacaggttac gtgccgggga caggatcggg ttcgtgcagg
     4201 agaagatatt caggcactac tgcatggtgc ccttttcatg ggagggtttt tttgaagtca
     4261 tctcggtcga aaaagccgga aagtacacga agaaccaggc aatcgcccat gtgaaggatg
     4321 agaaagggaa cgtgcgcccc gtaacgctct gccatcgatg gcctgtgaag atacccatcc
     4381 gggcatatgc cgagaagctc aagcccacgg aaccgctcgt caccaagatg cgcctcattg
     4441 attcaatctt ccctgtcgca cgtggtggga catactgcat ccctggtcct ttcggtgcag
     4501 gaaagactgt actgcagcag ctgatcagca ggcacgcaga tgccgatgtg gtggtcatcg
     4561 cagcctgcgg cgagcgtgcc ggggaggtag tcgagactat caagacattc cccacgctga
     4621 tcgatccgcg gaccagccgc ccactgagcg accgcaccgt catcatctgc aacacgagct
     4681 cgatgcccgt tgcggcaaga gagtccagcg tgtacacggc ggtcaccatc gcggaatatt
     4741 accgccagat gggcctccat gtcctgctgc ttgccgattc cacctcccgc tgggcccagg
     4801 ccatgcggga gatgtcagga aggctcgagg agatccccgg ggaagaggcg ttccctgcgt
     4861 acctcgagtc ccatatcgct tcgttctacg aaagggcagg gctggtgagg cttcacgacg
     4921 gatccctcgg gtcagtgacc atagggggtg cggtgagtcc cgcaggagga aatttcgagg
     4981 agcccgttac ccaggcgaca ctcaaagtgg tcggcgcgtt ccacggtctc tcccgcagcc
     5041 gctccgacgc ccgccgttac ccggccatcg acccgctcat cagctggagc aagtacggga
     5101 gtttcatcga ccgggagcag agggagacgg gcaggtggct tctccgtaaa ggcaacgatg
     5161 ttgcacagat gatgaaagtg gtgggagagg aggggacttc ccttgccgat tacacggttt
     5221 acctgaagtc ggaattcttt gactttgtct atctccagca gaacgccttc gaccctgtgg
     5281 atgaggcgac ccagaaagag aggcagatct acatcttcga ttttatttac cagataataa
     5341 aatcggattt ctccttcaaa aataaggacg aggcgcttca tttcttccag cagctccggc
     5401 agctcttcag gggttggaac tctgccccct ggcagagcga agaatttgag agaactgaac
     5461 aggagatccg tgggctcctt tccgaacgga tgaagacggg atagcatgca gaaattttat
     5521 tcgaacatca cccagatcgt cggcgatgtg atcacggtcg aggcagagaa cgtcggcaac
     5581 atggagcttg cccaggtgac cacccggcgc gggacctctc ttgcgcaggc gatacgactc
     5641 gatgggaaga aagtgttcct gcaggtgttc gcgggaagcc gtgggatctc caccggggac
     5701 aaagtgcggt tcctgggcca cacgatgcag atacccaccg gggacgcgct ccttggccgc
     5761 atcttcaacg ggagcgggag tcccctggat cgggggccgg cactcgatga gaacctggtg
     5821 gaagtaggcg gaccgccagt gaacccggtg aagaggatca tcccggacaa gatgatccgc
     5881 accggtatcc cgatgataga cgtcttcaac tctctcgtag aatcgcagaa gctgcccatc
     5941 ttctccattg caggggagcc atacaacgag ctccttgccc ggattgcaat ccaggccgag
     6001 gtcgatataa tcatccttgg cggaatgggg ctcaagtatg acgatttcat cttcttcagg
     6061 gagacgctcg gggagcatgg tgcactctcg cgttccatcg tgttcatgca taccgccgca
     6121 gatccaatcg tcgagtgcct gctcgtcccc gatatcagcc tcgcggtggc cgaaaacttt
     6181 gccctcgcgg gaaagagggt actcgttctg ctgacggaca tgagcaactt ctgcgatgca
     6241 ctcaaggtta tctcgatcac catggagcag atcccatcga accgtgggta cccgggcgac
     6301 ctgtacagcc agctcgcggc gcggtacgaa aaggcagtgg atttcgacac tgcagggtca
     6361 atcaccatcc tcgccgcgac cacgatgccg ggcgacgatg tgacgcaccc gataccagac
     6421 aacacggggt acatcaccga ggggcagttc tatctcaaga acagcgtcat tgagccgttt
     6481 gggtccctga gccgcttaaa acagcaggtc aacgggaaga cccgggacga ccacaggacg
     6541 atcatggaca ccatgatcca gctctttgcc gagtaccggg agactcttga gaagcaggca
     6601 atgggcttcg agatgagcgc ctgggaccag aagctcctga agtacggcag gctctttgag
     6661 gagaagatga tgtcccttgg ggtaaacatc ccgcttgaaa gagcccttga tctcggctgg
     6721 gagatcctcc acgaatgctt ccttcccgag gagaccggga tcaagagatc gatgatcgac
     6781 gcatactggc caggtacgca tgaaggttaa actctcccag ggggaactca agcggcagag
     6841 ggatgcactg cgccagtatg aacggtacct ccccacgctc caactgaaaa agcagcagat
     6901 ccagctcgag atcctttccc agcagggcgc catgagaaaa gctcgtgctg cgctctcccg
     6961 gaagatggag gctgcctcgg aatggtccgg cctccttacc gaggaccctg gtatcgcaag
     7021 gtggctcctt ccagaacagg tcgtgacagc gaggaaaaat gttgcaggag tcgacctgcc
     7081 tgtcttcgag agggcggagt tcgagacggc agagtatgac ctcttccttg cgccgctgtg
     7141 gcttgatcgg ggacttgagg tgctgaggga gatcgtgtca ctgcaggaag agatcagggt
     7201 ccttgagaca ggtatttcga tccttcacca tgacctgcga atcaccaccc agcgcgtcaa
     7261 cctctttgag aaggtgaaga tcccggaggc aaaagaggcc atccgcctga taaagatcca
     7321 cctcggcgac caaatgacca atgcagtggg ccgaagcaag attgccaaga agaagattga
     7381 ggcgtcaaat gccagcgagg cggggccatg atagtcccga tgaagaaagc gagcatcctt
     7441 tgcgaggccc gggatgcaga ttccacagtg aaaaccctgc gaagacttgg cgtcatgcac
     7501 gtggagcacc agaatacccc cgggaacgtg gacatcaccg ggcttgagga gcaggtcgcc
     7561 ctcatcgact ctactctcga tgtgctggac caggtggcag catcagaaaa ggaaataccc
     7621 aacgaagaaa ctctccttga cgcggactgg aaggcggttg cagatcgggt catcgaactg
     7681 ggtagacgca acgaccagac tgagatcctg tcaaggggca tcatcgcgca gataaacgag
     7741 tgggagcgct ggggggacat tgatgccgca gagatccgtg agctcagtga gaaggggatt
     7801 cacctctccc tttgtcaggt cccgcgaaag gagataaaga attttccgga cgacgtggtg
     7861 gtcaggacca tttttactac tggcgacatc gccaactgtg ccgtgatatc gaggagggcg
     7921 tttgaatgcc cttttcccga gattactccc ccgaagcagg gcgtatctgc gttgaggcgg
     7981 caactgcagg acatgacggc cggaattgaa ttggcaagga gagaactcct tttttgcgcc
     8041 cggtatcgta actcgctctc tgcaatcagg attgcccttg agagggagat cgagttccac
     8101 caggtactga gcggaatggg ccgggaggga gagatcaatt acgttaccgg gtatatccca
     8161 aatgacgccg aagagcagct gatcgaggca gcgagagaga agaaatgggg cgtgatgatc
     8221 acagtgcctt ccgatatcga cgaggtcccg acacttctga ggaaccctaa atgggtcagc
     8281 ctgattaagc ccgtgctcga ccttctcggg ctcacgcccg gataccggga acttgacatc
     8341 agcctcctct tccttgtctt cttcagcata ttcttcggca ttctcatcgg tgacgcggga
     8401 tacggggtgg tgtatatcct catcaccctg gtgctgcaga aggttgtgct gaagagctgg
     8461 gttctcgatc accctgaaat gaaaacgaca tttgcgctgt tttaccttct tggtgcatgc
     8521 ctggtgatat ggggccttct caccggagtc ttctttgggc agtcctggct gctggaactc
     8581 ggatacacgc ctgtagtgcc gcagttgaat gatccccaat tcatgcagac cttctgcttc
     8641 ttcctcggcg ccctccacct ctcaattgcg catttgtgga gagtgatact gaaatttcct
     8701 tcgcttcggt tgctcgcaga catcggctgg atatgcgtgc tctggactgc attcttcctt
     8761 gcccggacac ttatcctggg tgatccattc cctctgcttg gcatatggct gatcatcgca
     8821 ggaattgtcc tcgtcatcct cttcacccat ccccagcgaa atgtcctgaa aggtatcgcc
     8881 acgggactcg ggacaatcgc gctgagcctt gtgaacaact tcaccgacgt catctcctac
     8941 atacgtctct ttgcagtcgg cctcgcgacc ctggctattg cagaaaccac gaattccctt
     9001 gcatttggat tcggagacgg catcgtggca cttgtcgtgg gcgtcgtaat tcttattaca
     9061 ggacatggat taaacatcat tttaggaccg atgtccgttc tcgtgcatgg cgtcaggctg
     9121 aatgttcttg aattttccgg acatgcaaac gtgatatgga gcggaacggc atatgaacca
     9181 ttcaaggagg agtaacaccg ggagattacc atggacgcga acttactgac acaatttcag
     9241 gaccttggta tggccatggt ccttgcactt tccgcctgcg ggtctgccgc ggggacggga
     9301 acggcaggaa tggccgcgat aggggcatgg aaaaagaact acagccagaa caaacccgca
     9361 gcattcatgc tcgttgcgtt tgttggtgca ccactcaccc agacgattta tggcatgatc
     9421 gtgatgaacc agatgtctca actcgcaatg cagggcgtct tcctctgggg aattgggggg
     9481 ttctgtggtg cggcaatggg cctttccgca tatttccagg ggaagtgcgg tgcctgcgcg
     9541 tgtgattcga tgggagagac cggaaagggt tttggcaatt acatgattgt ccttggcatg
     9601 gtcgagaccg tcgcattgct ggtgatggca ttcacgctcg gcatccttgg cagttttggg
     9661 acatgagaga gccggatgaa aagcgggagc gtgagggata actcaaattt ttttctttaa
     9721 tttcaggaga ccatcgcaca tacatcccag ggatcattcc gggacttgat aattcacaac
     9781 atcatgtgaa atactccttg gtcattatgg caggtagcag ccctggtcct ttatccgcgc
     9841 ccgtgcaata acgcggagaa cagagattcc ctgcccaaaa aaacccattt tccgggagat
     9901 tcggtttacg tatcctttga tcggacgata atcacaggtc ttcttgagtt cactgcaacg
     9961 gcacccgcgg tactgccaat caggagctcg gcgaaccagc ctctgccgac agagctcatc
    10021 cagataactg acacgtcctc ctcctcggca acctttgtaa tctcgaatgc agggtttcct
    10081 agcctcacga tggccctggc ccggataccc tgcgcggcga actgcttgcg gattatctcg
    10141 atcttctccc tcgctctccg ctggttatcc tcgatctctt cttttgtctc gccgcgcgaa
    10201 acgacacaga ggagaatggc ctctcccacc ccgggagtcg cgtggagcct cgcgatcgca
    10261 tcgtccgaat aggatgagaa atcgacaggg cagaggacac gtgagaggat catcgggcag
    10321 aacatctcgt aggtcttccc gtcgaggccc tgggtgatct tgtggcgcat gatcagcacg
    10381 cttgtgcggc tcctgcggac gactgccttc gatacactgc caaggaggat tccctcgaca
    10441 aggctgctcc cgcgtgcgcc aatcaccacg agagagactc cgttctcctc tgccgcctgg
    10501 acaattgctc cggcaatgtc tgatgcaacc ttcaccactg tctggaccct gatgttctct
    10561 ccgaggctct cgaggtggtg tttctcctcc ctgagcgtga tcctgccatc atggaagagt
    10621 gccttcccta tctccccgcc ccctctcgga gatcttgtct ccttgaccac gtgcagaaga
    10681 accacctccc ggacaccggg aaaccccgcg atacagtcga gcgtcttcgc tgcatattca
    10741 gagaaatcgg tgggaaacag gatctttgag aacatgcgct ttcctgatct accgttgcat
    10801 gaccccttat attagtggat cgccttcgcg cctcctatcg ccatggcgcg gggataagcc
    10861 cggattccgg agggggacgt ggcgaggacc tatccgcact cctatccttc ccggacacgg
    10921 caggtgaata ccaaggagat tattttgaac cggacgggta taccagtgcg gggggcccgc
    10981 attctgtcct ttcccgcacg cagaacacga aaggggggtg aaccccatga aaagtgtcgt
    11041 cgccggcacg ggattcgctt ccctgcatag gaattttcag aaaagtaata ttctttcctg
    11101 tattgctcag cagaagagcg atgagaaaag ccacggattc atcttcattt cagccaatat
    11161 tagtggattc aggcatttgc tgcccgtttg gtcctttctg tggccagatc tcaccaatga
    11221 atttatgtag tagtgtatac attgcgcgca ttcacacgca aaatcacgaa caaattaaaa
    11281 aatataaaaa attaactgaa tttttaaggg ggactgtagt aagatatata tcctaaaata
    11341 gatacttagg tagtccttca gagggaggag tggatgcaat atggtcaacg agaaggcgct
    11401 ccagacaatc gagaaaatga aacttcttgt cctgaagcgg tgcgaagagc gggtccagaa
    11461 cgcttcggtg agttcgcact acacacctcc cgaaagattc ccctatccac agtgccacgg
    11521 cgtaactgcc atacttcgcc atcgccacgg actctaattg gtagaaggat taaaaaagcc
    11581 catgttatgc caaaaacacg ggtcgagcaa ggacgcagtg acattcgatg acctgtatgg
    11641 ataagttcgg gctgatgagg aagttgaacc gggattcagt aaaccaattg aataaatact
    11701 tggtagtgtc gcgaatgaaa catcgcggtg agagtgccac gccagaggtt cggagtaggt
    11761 gcagaacatt gggacgccct gcggaaatca tgcgcggaat gggagtgttg tgacatggcc
    11821 accgaaaagt cgttattgac aaaaatccgg gagaaagaac ttgaagtgag cgtaaagatc
    11881 gatgatgccc ggatggaggc agatcgaatg atcgagcgtg caaggaaaga gtcccagttg
    11941 atactcggcg catccgaaac agaagggaag aaagcagcag gggattttct gcgtaaggag
    12001 atggaaagga ttcaggcgga ggcggatcaa atccgggtgc aggcaacgag ggaggttgaa
    12061 tcagtccgga aaaaagggga acaccggatt ccggatgcag ttgaaaagat catcgccata
    12121 atacttccag gatagagagc catgctccag aaaatgataa aaatccaggt actgggtcca
    12181 aaaaaagacc tccataatgt tctggatgta ctctatgcca aggggactgt tcacatcgaa
    12241 gatgcctctg gaccaagttc acccggggag gtcacgctca agaggatgac ggcagagcag
    12301 gttggggaca tcaacgttgc gtattcaaag attggcggca tccttctcac acttccaaaa
    12361 acgaaaattg atgaaaaggc cgttgcccac acctatgcga atctgaaggc catgagccac
    12421 gataaccttc tcatccacgt gaattctgtg attgacgatc tggaagtggc cacaaaaaca
    12481 cttgcgaaag agaagagtga tcttgaactc tccctgatca accttggacg atatgaaaaa
    12541 atcatagaca aaatacgtcc ccttgaaacc agcatgccca aacttgaggg atttgaggtt
    12601 acggtcctgt taatacagaa ggagttcaag gacgtcatcg acatcatcag ggatgctctt
    12661 gcggagatca ccaagcgcca gtttgaatta ttgtcagccg aactggatga aacaactatc
    12721 gcggcagtca ctatctttaa taagcggttt tccgaccagg tgcattccta ttttttcaac
    12781 cagaacgtaa acgaagtccg cctgcctccc gagtacctgg gaaaatcgtt caacgagatc
    12841 ttctccctga tagaggagaa aaaaatctca tcactgaaag agatcgcaag actagattcc
    12901 gaacttgaaa agatctcttc gaaatggtac acggaactca acgcgttaca ccggatcctc
    12961 ggagaaaggg ccgaagaact gagtatcttt tcaaagatcg ggcagaccga atacacgttt
    13021 atgctggcag ggtggatacc caaaaaatcc ctcagggaga ccagggaagc cctgatggaa
    13081 ggattcgggg gacgtgtgat tgtcaatgaa ccggcactta cacccgagga gatggcggaa
    13141 gctcccacat tctatgacaa tccgcgcatt gtcaaaccgt ttgaattttt aatgtgtgtc
    13201 gtaagtcctg caaaatccac ggagtatgat cccagccccc tgatggcaat atttctcccg
    13261 ctgttcttcg ggatcatggt gggggacatc gggtatggat tgatcatcgc ggcgtttgcc
    13321 ctcatcatga aatggaaatt ctcggaccgt gactggctgc aacacctgat gaacgtcctc
    13381 atactgggcg cgatctggac gatcatcttc ggatggctct acggggaatt tctcggagac
    13441 cttggcgaac acatgggatg gatcgaacca aagacatttt ttggcgtcac ctggaaccgg
    13501 atggaggcga tagtcccctt cctgatcctc tcattcgcga tcggtgtctt ccatgtgttc
    13561 cttggccttg tgctgggcat agtgaacgcc tggacggaga tgagttgcaa gcggcacgtg
    13621 gacaaggcca agaagcacat ctgtgagaaa tcgggcatga ttatcgtgat caccggtctt
    13681 atcctggtga tccttggaat tgccctcgcg ctcccatcca gcattgtgac cacgggggcg
    13741 gtcctgataa tcgtggcaat ccccctcata ttttacggcg gcggcgcctt tggcacgttt
    13801 gagatcatga gtacagtgac gaatattctc tcgtactccc gtatcatggc tatcggcatg
    13861 gcttcggtca tacttgcggt tgtcgccaac aagctgggtg gcatgatgga ggttgccctt
    13921 gtggggctga ttattgcagc actccttcac acgctcaacc tgatactcgc catgttcagt
    13981 cctttcctcc attcccttcg tctgcacctg gtcgagttcg attcgaaatt ctaccaggga
    14041 ggcgggaaga tgtacaaacc cttcaagaag gaaaatggag gcaaatcgac atgaaccgcc
    14101 cggatgggtc tttacgtaca ttcatgatcg aaatacatgc cggagaggag tcatccatgt
    14161 aattgaagca tatcacacgg gcatattgtc taaatcaccc taatcgtgat gcggaacgca
    14221 gaggcacacg atcagaatcg aatctcgaaa acgatgtgcc gcctgtgcac atggcggcgc
    14281 tttggatgga ggctgatgtg aatggttgat ttcggaatcc ctattggggc aggcatcgca
    14341 ttcgggcttg gagcgctcgg taccgggatt gcacagtcaa gaatcggcgc agcgggtgcg
    14401 ggaacgattg cagaaaaacc agagatgttt ggattgatga tcattctggt ggctatcccc
    14461 gagaccctcg tcattctcgg ttttgtggtt gcatccatga tcatgatcat gctggtgtga
    14521 accgggagac tccaatactc ctgggaccag gggatctgga acggaacacc cgaaagaaca
    14581 ggtgaaccat ggcaattgac gatctgatca aggctgtaga agtgagcggg caggaacgta
    14641 tcaaggagat acaggaacgc tcgaaggccg aggcagatga aatcatcagg gaggcacatg
    14701 taaaagacct gcccataaaa aaacgcttca tggacggggc gatacaatcg gttgcagtac
    14761 agagaaacaa gatattatct gaagcacggg aaaagagccg gatggagatt atccaatcca
    14821 aaaacgaggt tttcgagaga gtttttgaag aagcggttca gaaactggca tccatacgaa
    14881 aagatccggg ctacagggca attctgaaac accttcttct ggaatcgatt ggtcaactcg
    14941 ggtcagacag cgtcattctt catgttgaca gaaaagacga agatctgttg aaggacatcc
    15001 tgaaggagat aaacgtatcc ggccagatcg tacccgatct gacctgtgcc ggcggcctga
    15061 acgcatacag tcgcgatgaa cgcttcgtga tcttcaatac gctcgaatcc cgtctcaaaa
    15121 aggcaaagga gatctacagg cccgaaatgt tctcggtatt gttcggtgag tgacatggag
    15181 tatgcgtatg tcaatgcacg gataaaggcc atgaaaagcc gcctgcttga ccgctcgttt
    15241 ctcgaacaac tcatcaacaa gcctgacatc gattctctca tcgctgaact tgaaaaaacc
    15301 acgtacaggg aggagctgga caaggcaggg gttctctata caggtattca ccgcattgaa
    15361 gtcgcgattc ggaaagatct tgtcaagaca ttcagaacgg tcctcaatct gatacgag
//
LOCUS       DAOQYR010000051        15416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_4296_length_15416_cov_9.274787, whole genome shotgun sequence.
ACCESSION   DAOQYR010000051 DAOQYR010000000
VERSION     DAOQYR010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 15416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15416
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4296_length_15416_cov_9.274787"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PKM87_07230"
     CDS             <1..778
                     /locus_tag="PKM87_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29896.1"
                     /translation="MKHHGDIFAVAEVIRDYKMKSFIDPEMALCGLNTISRKAEQVTR
                     GKITRACILASEGVRDRAIYRRAAHTYEIITGGKSVADATRILDEERKNYVERRGSAI
                     LSAFTGKKIELKFTAIKPHGRRTDKFTERYWGFDSNVSYDVTIDGKKYHVENLSGKEV
                     PDFALKGKNRDNPDWPVALFCGAVLTQELQYIGHTIINITVPAAVGAILGMDAGEAAD
                     KAEDGAFLTRAIPGAGDKAKDVAKLAQRVYAKINEPFPPQ"
     gene            779..1984
                     /gene="larC"
                     /locus_tag="PKM87_07235"
     CDS             779..1984
                     /gene="larC"
                     /locus_tag="PKM87_07235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HNQ29897.1"
                     /translation="MKALLIDPRTGGIAGDMLCAALCDLTGSEKPLQEVADAITGLSR
                     CSTFEISVERTVLPCSAACISVAIVEDDGPGTDVSRELNEVISTIGLSPSGTAMAHAI
                     LNDLISVKDRFHPETATGQTFASLDTIFDILGPLALLEEAGLLDCPIYTTPPALGGGL
                     IHTPGGDVGGPAPASLEICARHRMPVSDSALSMELTTPTGAALLANLARVVTLFPAMT
                     PLRTGYGAGAGDHGSSCRILRVVEGEIDDLTEERIVLLETNLDDVDGETIGYTRERLF
                     SAGAVDVFITSAQGKKNRPVNVLSVITTREDYLPLVRLLMDETGTLGVRIREEPRLVA
                     DRVNEKIRVLVRNQPFTVRIKISRSLGKVIAVKPEYEDLKQIARELGIPLRHVAREVY
                     QQLPFEYMR"
     gene            complement(2063..2596)
                     /locus_tag="PKM87_07240"
     CDS             complement(2063..2596)
                     /locus_tag="PKM87_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29898.1"
                     /translation="MDHPPPHAFSRSPVEEVICRCTLGRDAGSLSMSIPGPYPGWPHI
                     RERIREMIAGAEDISRITGCMLQYTDLIPVPQSMDLPGMKDIEQLLSGKYNCHTVQDE
                     VMLFRTHIPGTAGSIRSHLNRPGKPVWTLVFSIQTEGPARFASCEDLLDWFDDARAGI
                     HALFDIIVPEEIIQALK"
     gene            complement(2738..2986)
                     /locus_tag="PKM87_07245"
     CDS             complement(2738..2986)
                     /locus_tag="PKM87_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29899.1"
                     /translation="MSKIRTFFLIGLLVLLIGVVVGVVGMVMADTNLLASSQFFLIIS
                     MIIMLWGYVITLDNIDKNVARNVELMKSLLDTMDKGQK"
     gene            complement(3062..3196)
                     /locus_tag="PKM87_07250"
     CDS             complement(3062..3196)
                     /locus_tag="PKM87_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29900.1"
                     /translation="MIIRSSSCGEALVDAIRILRGAVELYIETFAGKDIVTRGTVISG
                     "
     gene            3261..3488
                     /locus_tag="PKM87_07255"
     CDS             3261..3488
                     /locus_tag="PKM87_07255"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNQ29901.1"
                     /translation="MFSHHTKTGYRESLPGIRQKTLVFGEHTLMTEFRLDMGSNLPAH
                     THPHEQTGYLVSGHITLRIGEKESEIRPGDR"
     gene            3821..4387
                     /locus_tag="PKM87_07260"
     CDS             3821..4387
                     /locus_tag="PKM87_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29902.1"
                     /translation="MIPILNPGQYPAILANGFLIIVMMGVPTAFLSLCIFFLLERIPH
                     RGARLILPAAGAVLIFGVSLFFFSGVPQSPEEYQETWVPMILTSFLLNALVILAPFPF
                     IRPYIRRYTPYLVIPATLVVTFFLLVAFGLMGGDAQVPPDTEVEIMAGKVTYAIAESV
                     FATLSYGCVAVLERIFPGKSGSHQQRTR"
     gene            4463..4672
                     /locus_tag="PKM87_07265"
     CDS             4463..4672
                     /locus_tag="PKM87_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29903.1"
                     /translation="MKGYTDYQRREYCRDVKCPIQLLLNKEVEKSPKYEEIRAICASN
                     CLHTTHEFHHWLIQKGYLVVRPEEK"
     gene            4723..5547
                     /gene="lsrF"
                     /locus_tag="PKM87_07270"
     CDS             4723..5547
                     /gene="lsrF"
                     /locus_tag="PKM87_07270"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: protein motif:HMM:NF006081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HNQ29904.1"
                     /translation="MATAYLDVGIQSRMNRIINPKDNRVVMLAVDHPYFQGPTTGLRN
                     MSATIKTLLPYCDCLMTTRGAVRANIAPEDLGSKPIMLRVSGGNSVLFDELSDEQITV
                     SMEDAIRMDAAGVAVSVFIGSANQQQTILNLTDMINSAEEYGIPVLAVTAVGKDMARD
                     LRYLGLASRICQDAGARIIKTYYCEDFSKLVDWVAPTAVVVAGGKYSSPPEALQMAYD
                     SIQAGAVGVDFGRNIFQDENPVGMIKAIGSIVHGNHTVKEALDIYKACMPGAKKLE"
     gene            5617..6093
                     /locus_tag="PKM87_07275"
     CDS             5617..6093
                     /locus_tag="PKM87_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNQ29905.1"
                     /translation="MKSLVIVYSYHHHNTEKIAQVLANILDAPVKKPDEVNPEEISEY
                     GLVGFGSGIYYGRHHESLLSLADRLHKVSTGTAFLFSTAAITGDAKTSKDHSRLREKL
                     QSKGYVIAGEFGCKGFNTYSFLKYIGGMNKGRPNADDLHRAEEFAMSLKKDYLTGK"
     gene            6149..7201
                     /locus_tag="PKM87_07280"
     CDS             6149..7201
                     /locus_tag="PKM87_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNQ29906.1"
                     /translation="MKAAVYYSNSDIRIEDVDDIAVSPGEIKVKVMACGVCGSDVMEW
                     YRIKRAGRPGGIGAFGHECTGVIEEVGSGVDPKWKVGDRVVVTHHVPCNTCNACLRGH
                     ATACETLQKTKFKNAYGAFAEYVVLPAINVDRGCLHLPESVSFDEGTFVEPLGCVVRG
                     QRFAPTSDGRSVLILGAGITGLLHLKAARLNGAGFIAVSNPSPDRLKIARTFGADATI
                     SAKEDVPERFKELNGGSGADTVIMTAPVPVCVKQSLDAVGRGGTVLFFAPTNPEIQSG
                     INLWNLWQKEVTITHSYAADLHDLTTAFTWIKYHRIDVTDMITHVFPLRDTVEGFQIT
                     ARHKEGSLKAIVHPQE"
     gene            complement(7418..7690)
                     /locus_tag="PKM87_07285"
     CDS             complement(7418..7690)
                     /locus_tag="PKM87_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNQ29907.1"
                     /translation="MKYRILYTSRARKDLRRFPDIVARRIIRAIHTLGEEPYLNVKKL
                     KGGALDHPVYTFRIGLYHRAILSIHDEVLVIHVLEIEDRKQAYRDF"
     gene            complement(7687..7923)
                     /locus_tag="PKM87_07290"
     CDS             complement(7687..7923)
                     /locus_tag="PKM87_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VapB family protein"
                     /protein_id="HNQ29908.1"
                     /translation="MQAASTIYIREDLKNKLQELKHHPKESYNDVIERLVSLSIDSEP
                     LNDDAIRGLEEAISDIKHRRLHPEEEIMAEFGIQ"
     gene            8423..10540
                     /locus_tag="PKM87_07295"
     CDS             8423..10540
                     /locus_tag="PKM87_07295"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HNQ29909.1"
                     /translation="MSPRRRILLLSSCLILVAIIAAGCMSIPATTPTVPPGIKKFNST
                     AEIEQYLEESMAHFEHDAAYRTMVPTVGISADSKAMAQSEQTSGIPAPSTRGSGPGAS
                     GVPYSQTNVQVAGVDEPDIIKNDNRFIYTISGQNLVIIDAYPAADASVISTHEIADTP
                     RDIFVHGDRLILFSTGTPDPDTYPERSESRMMPAYYIPSPPITHATIYDISDRAKPAV
                     VKDYSIDGDYLDARMIGSLVYLVTQEQIYPYYRDSPIIVPALREGSRTVIQPDVWYFD
                     HPESQYTFTTITALDIASGNERDAQTYLLGSGNTLYVSEDALYVSYPQYRPVIYYRGQ
                     EDLPVKAVAVSDSAARSSVIPTDFNTLTGQERQSILEGLREDEQDAIRRQEVAQTNTV
                     LHKIGIRNGAITYRAKGEVPGTLHNQFSMDEYDGNLRVATTSSVYTPRYGQYTYNNVY
                     VLDHNMATIGELTHIAEQETIYSTRFIGDRLYMVTFKRIDPFFVIDLSNPAIPKVLGK
                     LKIPGYSDYLHPYDATHIIGIGKETSTNEWGGVSTSGVKLALFDVSDVNNPTQIDKVQ
                     IGDAGSDSAALEDHHAFLFDRAKNLLVIPIRAVTNEPATQEHSLSAYRPYIWYGAYVF
                     GLSPETGFALRGTVQHGSGDSGYYYYGSSASEVKRSLYIGDVLYTMSSKQIKANPLDD
                     LATTLATVSLPPAGDIYYPVVVK"
     gene            10719..11210
                     /locus_tag="PKM87_07300"
     CDS             10719..11210
                     /locus_tag="PKM87_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29910.1"
                     /translation="MQKFYVILLAGLLSVMLIASGCTSPSPAVTTTPVPTTGAPVATT
                     PMVTTPAVPSWAGTWNTTWLNTDGNLTVSVVTMTAAGQEVSGNYSYTYPDEGPFTGHL
                     NGTAKGQTLAGMYSESDDDAGYIIFELSGDGNSFSGRWVHAEDQTELENSTLFWNGVR
                     IAP"
     gene            complement(11218..11940)
                     /locus_tag="PKM87_07305"
     CDS             complement(11218..11940)
                     /locus_tag="PKM87_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNQ29911.1"
                     /translation="MGISERRQREKEQRRTEIVDAAEQLFFSRRYEEVSMDDIAREVE
                     LNKATIYLYFESKEALFATIVLRGVRILEEKYRECMDTQVPGIEKVALMGRAYSRFSE
                     EYPEYLRLIHFYGSERFSRENPYTAEIGKGYGTCRGILQDAIREGIDDGTIRNDLNPF
                     LASMYCMISFMGILSLEPKWKLVIEDEGFSSEQFTHEFFRFITPALSSGGTPHTMDER
                     EGSGPLSATPEPARKKKRKKLE"
     gene            complement(12037..12903)
                     /gene="nudC"
                     /locus_tag="PKM87_07310"
     CDS             complement(12037..12903)
                     /gene="nudC"
                     /locus_tag="PKM87_07310"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HNQ29912.1"
                     /translation="MDATRRSDMEHYPLYAVHPLHSVFPEPEDLSPAQRRWILVQNTS
                     VVFRDEPAPGTVVMGDLLPEGLASGAPVYLGTDNDLCYYAAEVASGATLPPGWETSPV
                     RELHGVVPDRDMAYAAYAIRILDFDRSNTFCGKCGANTRPSTTERARICTSCSRIVYP
                     RVSPAIIVLLKKGEKVLLARSPRFTPGVYSILAGFNEPGENLEQTVHREVGEEVGIAV
                     KNIRYFGSEPWPFPDSLMIGFVADYDSGEIRIDPREIEEASWFSRENLPPFPSKTSIS
                     RALIEAWRRGEV"
     gene            complement(12939..13763)
                     /locus_tag="PKM87_07315"
     CDS             complement(12939..13763)
                     /locus_tag="PKM87_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037207.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HNQ29913.1"
                     /translation="MSIESARLVYFSPTGTTQAVVRAIARGINHSNFEELDITRPDAR
                     VQPLQASDNELLIIGVPVYMGRVPALVTEWLSVIKARNTPAVCVVVYGNREYEDALLE
                     LSDILIKGGCIPIAGSAFIGEHSFSTDETPTAVGRPDANDQDFAEVFGKNIWEKLRSI
                     SSLDRMSEVTIPGCRPYRQEPCHPYRRDSVFWNVDFIEVSDACIQCGICAEVCPVGAI
                     DPENCRAIDTEHCITCCACIKHCPKHARTIKPGFVRDASVRLHTLYGTRKEPECFL"
     gene            14087..15301
                     /locus_tag="PKM87_07320"
     CDS             14087..15301
                     /locus_tag="PKM87_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HNQ29914.1"
                     /translation="MKLITVLITLCVLAGSAAALSMPPVSPLFPPARPDLVYVSSFEG
                     GHRLQAGDYPVIVLSGTFRQMGRQYGALMKAELNEEYRFISSAYEKRGYSNEQLRADG
                     REASVLYSKRLKEVFSGMAETSGLTQDEVLALYNGLVIYLMVPPADASCSYLSAWGNY
                     TTDGSVIASRNWDLDDVALPFNKWNVLVVYRPSDGSNGVATFGPAGVRAETVMNSKGL
                     FIADDFAGFIDPAQDTRPDLVAEFFRFMLDYSNLAELDVGLRGSATNIGWIVNVAGPG
                     GASVYEVTPNQTRQRTGDGVVAAANHFVDPAWNLPPPAEHSASRYDGLLREADEAKGS
                     IDARRMMQIRDVRWEDGGATFVNSVLSPDHPEELYSTIHQVVFLPESRTVWIKDPDSA
                     WQRVDLGMLFGE"
BASE COUNT         3490 a         4301 c         4178 g         3447 t
ORIGIN      
        1 catgaaacac catggcgaca tcttcgcggt cgccgaagtg atccgtgatt acaagatgaa
       61 atcattcatc gaccccgaga tggccctctg cggcctgaac accatctcgc ggaaggccga
      121 gcaggtgaca cgcgggaaga tcacgcgggc ctgcatcctt gcctcggaag gcgtgcggga
      181 ccgggctatt taccgccggg ctgcccatac ctacgaaatc atcacgggcg ggaaatctgt
      241 ggccgacgcg acccgcatcc tcgatgagga gcgcaagaat tacgtggagc gccggggttc
      301 ggcgatcctg tccgcattta ccggaaaaaa gatcgagctc aaattcaccg cgatcaagcc
      361 ccatggacgt cgcacggaca agttcaccga acgatactgg ggctttgact cgaatgtctc
      421 gtacgatgtc acgattgacg ggaaaaagta tcatgtcgag aacctgagcg gaaaggaggt
      481 gcccgatttc gcattgaagg gaaagaaccg cgacaacccg gactggccgg tcgccctctt
      541 ctgcggtgca gtccttacgc aggaactcca gtacatcggc catactatca tcaacatcac
      601 cgtccctgcc gcagtcgggg caatccttgg aatggatgcg ggcgaggcag ccgacaaggc
      661 agaagacggt gcattcctga cacgggcaat cccgggggcc ggagacaagg caaaggatgt
      721 tgccaagctt gcacagcggg tctatgcgaa gatcaatgaa ccgttcccgc cacaatgaat
      781 gaaggcgctt ctcattgatc cgaggaccgg ggggattgca ggggatatgc tctgtgctgc
      841 cctttgcgat ctcaccggtt cggaaaaacc ccttcaggaa gttgctgatg caatcacggg
      901 actttcccgt tgttccacct tcgaaatctc ggttgagcgg acggtcttgc catgcagtgc
      961 cgcatgtatt tcggtagcca tagtggagga tgatgggcct ggcactgatg tttcacgcga
     1021 attgaacgag gttatctcca ccatcgggct ctcgccgtcc gggacggcga tggcgcatgc
     1081 tattctcaac gatctcatct cggtaaagga ccggtttcac ccggagaccg caacgggcca
     1141 gactttcgca tctcttgata cgatctttga tatcctcggc cctctcgcgc tccttgaaga
     1201 agcaggcctc ctggattgcc ccatctatac cacccctccc gcccttggcg gaggactgat
     1261 ccatactcca ggcggtgacg tgggggggcc tgccccggca tcccttgaga tctgcgcacg
     1321 ccaccgcatg ccggtatccg atagtgctct ctccatggaa ctcacgacac cgaccggtgc
     1381 agccctgctt gcgaatcttg cccgcgtggt aaccctgttt ccggccatga cccccctgcg
     1441 caccgggtat ggtgcaggtg caggggatca tggatccagt tgccgcatcc tccgggtcgt
     1501 cgagggggag attgatgatc tgactgaaga gcggatcgtc ctgctcgaaa cgaacctcga
     1561 tgacgtggac ggcgagacga tcgggtatac gcgcgagaga ctcttttcgg ccggggcggt
     1621 ggatgtcttt atcacgtccg cgcagggtaa gaagaaccgc cctgtcaatg tgctgtccgt
     1681 gatcaccacc cgggaggact acctcccgct cgtcaggctc ctgatggatg agaccgggac
     1741 attgggtgtc cggatacgcg aggagccccg ccttgtcgcc gacagggtga acgaaaagat
     1801 cagggttttg gttcgcaatc aaccttttac cgtgcgtata aaaatctcca ggtccctcgg
     1861 aaaggtcatt gcggtcaaac ccgagtatga agatctgaaa cagattgcgc gggaactggg
     1921 aattcccttg cggcatgtgg cacgggaagt gtaccagcag ctcccttttg aatacatgag
     1981 gtgaccggca gtcaccactg cttcgggcct ttttcagaca aattttcccc aaatctccgg
     2041 agaagaccct tgccgggatg cattacttaa gagcctggat gatctcttca gggacgatga
     2101 tgtcaaagag tgcgtggatc ccggcccggg catcgtcgaa ccagtccaac aggtcttcgc
     2161 atgaggcaaa cctggccggt ccttcagtct gaatagaaaa aaccagggtc cacacaggtt
     2221 ttccgggcct gttgagatgt gaacggattg atcccgcagt gccggggatg tgtgtgcgaa
     2281 aaagcatgac ctcgtcctgt acagtgtgac aattgtattt cccggagagg agctgttcga
     2341 tatccttcat cccgggcagg tccatactct gagggacagg gatcaggtcg gtatattgga
     2401 gcatgcaccc ggtgatccgg gagatatcct ctgcaccggc aatcatctcc ctgatcctct
     2461 ccctgatgtg cggccagcca gggtagggtc ccgggatact catggagagt gatccggcat
     2521 cacgtccgag agtgcagcga catatgacct cctcgaccgg tgagcgggaa aaggcgtgtg
     2581 gaggcggatg gtccatcaag agaaggagta tgggggggtc gtccttctat attttctgct
     2641 ttcattgcag gcattcacga ttcgggagta ccagaaaaaa gcgccgcctg atgattccgg
     2701 aagtgcagta ctcgcgtaaa tacacaccct cacaacgcta tttctgtcct ttatccatgg
     2761 tgtcgagaag tgatttcatc aattccacgt tacgggcgac attcttgtcg atattatcca
     2821 gggtgatcac atatccccac aacatgatga tcatgctgat gatgaggaag aattgagagg
     2881 aggccaacag attggtatct gccattacca taccaacaac accgacaaca acgccaatga
     2941 gaaggacaag cagtccaatt aagaagaatg tgcgtatttt cgacatgaag ggatactatt
     3001 cttaaaagat tattaatttt attggataaa atttccaggt tggaattatt tccagacaat
     3061 tctacccgga aatcaccgtg ccccgggtta caatatcctt cccggcaaac gtctcgatgt
     3121 acaattcgac tgctccccgg agaatccgga ttgcatcaac gagtgcctca ccacatgagg
     3181 aactccggat gatcatagaa catgggatac gtcacagtat tctttatcgg ttcaaccggc
     3241 caatcccctg attgttctcc atgttttccc atcataccaa gaccgggtac agggagtcgc
     3301 tccccggcat caggcagaag acactcgtct ttggggagca tacactcatg accgagttcc
     3361 ggctggacat gggatcaaat ctcccggcgc acacccaccc ccacgagcag acagggtatc
     3421 tcgtgtcggg gcacatcacg ctccggatcg gcgagaaaga atcagagatc cggccagggg
     3481 acagatagaa cattcccgga aatgttgcgc actcggcaac gatccaccag gactcggttg
     3541 cagtcgaggt cttttccccg gtccgggacg aatgcctgcc gtaatcgaat aggtttttta
     3601 atgattcatt gccagctgca ggatatatct ttagtctgag tataccctgg aggaattcca
     3661 gtataatggt gagagtgacg tgtgggtgag agaaggaagt atttctctgt gcaggatatc
     3721 cctgaaaggc caggtctgcc gccttgatgt gttctcttaa gatctgaaag ccagtcatat
     3781 cctccgattc tatttatcta ccacaataca acatggaacc atgattccca tcctgaaccc
     3841 ggggcagtat cccgcgatac ttgcgaacgg atttcttatc atagtcatga tgggagtccc
     3901 aacggccttc ctctcccttt gtatcttttt tctgctcgaa cggatccccc accgcggggc
     3961 ccggctcatt ctcccggcag cgggtgctgt gctgatcttc ggtgtcagtc ttttcttttt
     4021 tagcggagtt ccccagtccc ctgaagaata ccaggagacc tgggtcccga tgattctgac
     4081 cagttttctc ctgaacgcac tggtgatact cgcaccattt ccgtttatcc gcccatatat
     4141 caggagatac acaccctacc tcgtgatacc tgcaactctc gttgtcacgt tctttcttct
     4201 ggttgccttc ggattgatgg ggggagatgc acaggtgcct ccggatacgg aagttgagat
     4261 catggccggt aaggttacat atgctattgc tgagtctgtt tttgcgacgc tctcatacgg
     4321 gtgcgttgcg gtgctggaaa ggatatttcc tgggaaatca ggttcccacc aacaacgcac
     4381 cagatgaaat ttgtaggtat ctgttgcggg tgagatcgat catttcgcaa gcctttttca
     4441 tttccattct caagaatctg gtatgaaggg gtataccgat taccagcgcc gggaatattg
     4501 ccgggacgtg aaatgcccaa tccagctgct cctgaacaaa gaagtggaga aatccccaaa
     4561 atatgaagaa atccgggcga tctgtgcatc caactgcctg cacacaaccc acgagttcca
     4621 tcactggttg atccagaagg ggtacctggt cgtaagacca gaggagaaat gatactaact
     4681 ttctttaccg ttgtgagtca ttaattctcg tgaagtgtcc ccatggcaac tgcatatctt
     4741 gatgtcggga tccagagccg gatgaaccgg attatcaatc caaaggacaa ccgggtcgta
     4801 atgctcgcag tcgaccatcc ctatttccag gggccgacga cgggtctccg gaacatgagc
     4861 gccacgataa aaaccctcct cccctattgc gactgcctga tgaccacacg cggggcggtg
     4921 cgggccaata ttgcacccga ggacctcggg tcaaagccta tcatgttgcg ggtatcggga
     4981 ggaaacagcg tgctcttcga cgagctgagc gatgaacaga tcacggtctc catggaagac
     5041 gccatcagga tggatgcggc cggggtcgcg gtcagcgtgt ttatcggctc tgccaaccag
     5101 cagcagacga tcctcaatct caccgacatg atcaattccg ccgaggaata cggaattccg
     5161 gtgcttgctg tcaccgcggt cgggaaggac atggccaggg acctgcgcta cctgggcctt
     5221 gcatcccgca tctgccagga cgcaggagcc cggatcatca agacctatta ctgcgaggat
     5281 ttctcgaaat tggtcgactg ggttgctccc accgcagttg tcgtggcggg agggaagtac
     5341 tcgagtccac ccgaggccct ccagatggcc tacgactcga tccaggcggg tgccgtggga
     5401 gttgattttg gccggaacat cttccaggac gagaaccccg tcggcatgat caaggcaatc
     5461 ggctcgatcg tccacgggaa ccacaccgtc aaggaagcgc ttgatatcta caaggcatgc
     5521 atgcccggtg cgaaaaagct cgaatgagta tggaatatcc ttcaaatact cagtgtttcc
     5581 ggttcactgg aacaagaaaa aacctgtgtg cctgaaatga agagcctcgt cattgtatat
     5641 tcctatcatc accataacac agagaaaatc gcacaggtac tcgcgaatat ccttgatgcc
     5701 ccggtaaaaa aaccagatga ggtgaatcct gaagagattt ctgaatatgg actggtcgga
     5761 tttggttcag gaatctatta cggaagacac catgaatccc tgctctccct tgccgacaga
     5821 ctccataaag tcagtacagg tactgcgttc ttattctcta ccgctgccat taccggagat
     5881 gcaaagacct caaaagatca ctcccgtctc agggaaaaac tgcaatcgaa agggtatgtg
     5941 attgctggtg aatttggctg caaaggattc aacacatata gttttctgaa atatatcggc
     6001 ggaatgaaca aaggaaggcc gaatgcagac gaccttcacc gggctgaaga gtttgccatg
     6061 agcctgaaga aggattatct gaccgggaaa tagtgtcctg ttgacggaat tattcaaagt
     6121 gtgaaatgtc atctctacgg ggaaccgcat gaaagccgca gtctactatt cgaacagtga
     6181 catccgcatc gaggatgtcg acgatatcgc cgtgagcccg ggcgagatca aggtcaaagt
     6241 catggcctgc ggtgtgtgcg ggtcggacgt catggagtgg taccggatca agcgtgcagg
     6301 gcgccctggc ggcatcgggg cgttcgggca cgagtgcacg ggagtcattg aggaggttgg
     6361 atcaggcgtc gatccgaaat ggaaagtggg ggaccgcgtc gtcgtgaccc accatgtccc
     6421 ctgcaacacc tgcaatgcgt gtctccgcgg gcatgcgact gcatgcgaaa cactgcagaa
     6481 gaccaagttc aagaatgcgt atggggcatt tgcagagtat gtggtgctgc ccgcaatcaa
     6541 cgtggaccgg gggtgcctgc accttcctga atcggtctcg ttcgacgaag ggactttcgt
     6601 cgaacccctc gggtgcgtgg ttcgcgggca gcggttcgct ccgacttcgg atggccgatc
     6661 ggtcctgatc ctcggagccg gcatcacggg actcctccat cttaaggcag cccgcctgaa
     6721 tggtgcgggg tttatcgccg tctccaaccc gagtcccgat cggctgaaga tcgcccggac
     6781 attcggtgct gatgcgacta tttccgcaaa agaggatgtc ccggaaagat ttaaagaact
     6841 aaacggcggg tctggcgcag acaccgtgat catgacggcc cccgtccccg tctgcgtgaa
     6901 gcagtcactt gatgcggtcg gacgcggggg gacggtcctg tttttcgccc ccacgaatcc
     6961 tgagatccag tccgggatca atctctggaa cctctggcag aaggaggtca cgatcaccca
     7021 ttcctacgcg gccgatctcc atgatcttac cactgcgttc acatggatca aatatcacag
     7081 gattgatgtg accgatatga tcacccatgt atttcccctg cgggacacgg ttgaaggctt
     7141 tcagatcact gcccggcata aagaagggag tctgaaggcg atcgtgcacc cgcaggaata
     7201 gatttttttc cagttctgaa aatcactgca acgcgaatta ttatccattc tcaccgttcc
     7261 acctgaacca tgcgtgggaa aaatccctac agcacgaagt ataccagaat acccacaggg
     7321 tatacatttc agaagatctt cactgcgcca gatcacgata ctaaagacga tgaaaatctg
     7381 cctgatgtgg caaattccac tgatgatgga tgcgtgatta aaaatcccga tatgcctgct
     7441 tcctgtcctc gatctcaaga acatggatga caaggacttc atcgtggatg gaaaggatcg
     7501 ccctgtgata cagaccgatc cggaaggtat agaccgggtg gtccagggct ccacctttca
     7561 gcttttttac attcaggtat ggctcttccc caagagtgtg gatagcccgg ataatacgcc
     7621 ttgcgacgat atccggaaat ctgcggagat ccttccgggc ccgggaagta tagaggatcc
     7681 ggtacttcat tgtatgccga actcggccat gatctcttct tcagggtgga gccggcggtg
     7741 tttgatgtct gagatcgctt cctccagtcc ccggatcgca tcgtcgttca atggctcgga
     7801 atcgatcgag agcgacacaa gccgttcgat gacgtcgttg taggattctt tggggtgatg
     7861 cttgagttct tggagtttgt ttttcagatc ctcccggatg taaatggtgg aggcagcctg
     7921 cataaagaaa cctataggtg tatatagata tcaatctgtc gataataagg gatcggaagg
     7981 agaaaccaac cattcccatt ctcctcacca ttaccaccat ttctatcgcc gggagcaaca
     8041 gcctgtgcat cctccatata acccttgccc agggtgcgat gaataatctc tccaggcagg
     8101 aaaattttgg aatatgttca agttcctggt cgtgattaaa aaagttcggt aagaggtatt
     8161 ttgcatacac gaatggatat gactgatatg atcacccacg aaataattat gcaaagacaa
     8221 ggtgcagggt ttttcccgta gccacccacc tcccggggca ggagtatgaa cgtgggtcca
     8281 ccattcccag gactggaatt cagcaggcag gggacctggc atcggtgcca agcgaatccc
     8341 gatgtgatcg tattgctgcg agatttttcg cagtttacgc cagattgatg tcctcatctc
     8401 ccctatggcg atgggagaga ccatgtcacc cagacgccga attctcctgt tgtcctcctg
     8461 cctcatcctt gtcgcaatca ttgccgcagg atgcatgagc attcctgcaa cgacgcccac
     8521 ggtcccgccc gggatcaaga agttcaattc gactgccgag atcgagcagt atcttgaaga
     8581 gtcgatggca cacttcgaac atgacgctgc ctaccgcacc atggtgccca ccgtggggat
     8641 cagtgccgat tcgaaggcaa tggcgcaatc ggaacagacc tcggggatcc ctgcaccctc
     8701 caccagaggt agcggtcccg gggccagcgg agtcccctac tcgcagacca atgtccaggt
     8761 tgccggggtc gatgagccgg atattatcaa gaatgacaac cggttcattt acaccatctc
     8821 cggccagaac ctggtgatca tcgatgccta ccctgcagcg gatgcatcgg tgatatccac
     8881 ccacgagatc gcggatactc cccgggacat ctttgtccac ggggaccgcc tgatcctctt
     8941 cagtaccgga acgcctgatc cggataccta cccggaacgc tccgaatcac gaatgatgcc
     9001 tgcgtactac attccttctc cacccatcac gcatgcgacc atctatgata tcagcgaccg
     9061 tgcaaagcct gcggtcgtga aagattactc gatcgacggc gattacctcg atgcccggat
     9121 gatcggatcg ctcgtctacc tggtcaccca ggagcagatt tacccgtatt accgcgatag
     9181 cccgatcatc gtccctgctc tccgcgaggg atcccggacg gtaatccagc cggatgtctg
     9241 gtacttcgac catcccgagt cccagtacac gttcaccacg atcactgccc ttgatatcgc
     9301 gagcgggaat gagagggatg cccagaccta tctgctgggc agcggaaaca ccctctacgt
     9361 ttcggaggat gctctctacg tgagttaccc gcagtatcgc cccgtgatct actaccgtgg
     9421 gcaggaggat ctcccggtca aggccgttgc cgtcagcgat tcggcagccc gttcgtctgt
     9481 catcccgact gacttcaaca cgctcaccgg gcaggaacgc cagtccatcc tcgagggtct
     9541 ccgggaggat gagcaggacg cgatccgccg gcaggaggtc gcacagacta acacggtcct
     9601 ccacaagatc ggcatccgta acggtgccat cacctaccgt gcgaaaggcg aggtgccggg
     9661 cacccttcac aaccagttct cgatggatga atacgacggc aatctccggg tcgcgaccac
     9721 gtcaagcgta tacacgcccc gatacgggca gtacacctac aacaatgtct atgtcctgga
     9781 ccacaacatg gcaacgatcg gcgaactgac ccatattgcc gaacaggaga cgatctactc
     9841 aaccaggttt atcggcgacc ggctctacat ggtgactttc aaacgaatcg accccttctt
     9901 tgtgatcgat ctctcaaatc cggcaatccc gaaggtcctt gggaaactga agatccccgg
     9961 ctactccgat tacctccacc cgtatgatgc aacgcacatc atcgggatcg gcaaggagac
    10021 gtcgaccaat gaatggggag gggtctccac gagcggggtc aagctcgccc ttttcgatgt
    10081 ctctgatgtc aacaacccta cacagatcga caaggtgcag atcggggatg ccggctccga
    10141 ctcggccgca ctcgaggacc accacgcgtt cctctttgac agggcaaaga acctccttgt
    10201 gatacccatc cgggctgtca ccaatgaacc tgccacacaa gagcactccc tctctgccta
    10261 ccgtccttat atatggtatg gggcctacgt cttcggcctc tctcccgaga ccgggtttgc
    10321 cctccgaggc actgtccagc acggcagcgg cgactccggg tactattatt acggcagctc
    10381 ggcatccgaa gtgaagcgtt ccctgtatat cggcgatgtg ctctacacga tgtcttcaaa
    10441 acagatcaag gcgaatccgc tcgacgacct cgccacgacc cttgcaacag tctcccttcc
    10501 tcctgcgggc gatatctact acccggtcgt ggtgaagtag aggagggaga ggaaaatttt
    10561 ttcctggact ttagaatcta atgaaaacct tgtaaagact acctgggttt atccgtgtgt
    10621 ggtgaggttg ttctctcatg aacgagaaat ccatcgggtt ctgacacgca cttcagcagg
    10681 gttaagagca atcggaaata attctgggag atgatcgtat gcaaaaattc tatgtgattc
    10741 tcctggcagg cctgctctcc gtcatgctga tcgcgtccgg ctgcacgagt ccttctcctg
    10801 cggttactac aacacctgtt cccacgaccg gagctccggt tgcgacaacc cccatggtga
    10861 caactccggc tgtcccctct tgggccggga cctggaacac cacctggctc aacaccgatg
    10921 gcaacctgac agtctcagtc gtaaccatga ctgcggccgg gcaggaagtc tccgggaact
    10981 acagttacac ctatcccgat gaaggcccct tcaccgggca cctgaacgga accgctaagg
    11041 gacagaccct tgccgggatg tattccgaga gcgacgatga tgccgggtat atcatcttcg
    11101 aactctcagg ggatgggaac tcgttttccg gccggtgggt ccacgccgaa gaccagaccg
    11161 agcttgaaaa ttcaacactc ttctggaacg gcgtcaggat agccccctga tgaaaaatca
    11221 ttccaatttt tttcttttct tcttccgggc cggttcgggg gtggccgaca gtggcccgga
    11281 tccttcacgc tcgtccatgg tatgaggtgt tccccctgac gaaagggcgg gggtgataaa
    11341 ccggaagaat tcatgcgtga actgttcgct ggagaacccc tcatcctcaa tcacgagttt
    11401 ccacttcggt tccagggaga ggatccccat gaaggagatc atgcagtaca tcgaggccag
    11461 gaacggattg agatcgttcc ggatcgtccc gtcatcgatt ccctcccgga ttgcatcctg
    11521 caaaatccct cggcaggtgc cgtatccttt gccaatctct gcggtatacg ggttctccct
    11581 cgagaaccgt tcggacccgt agaagtggat gagtcggaga tactcagggt attcttctga
    11641 aaaccgggaa tacgcccggc ccatcaaagc aactttttca atcccgggca cctgggtatc
    11701 catgcattcc ctatattttt cttcaaggat ccggacgcca cggagcacga tagtcgcaaa
    11761 gagagcctcc ttgctctcga aatagaggta gatggttgcc ttgttcagtt cgacctcgcg
    11821 ggcgatatcg tccatggaca cctcctcgta cctcctagag aagaagagtt gttcggcggc
    11881 atcgacgatc tcagtccttc gctgttcctt ctcccgctgt cttcgctcgg aaattcccat
    11941 gctcttacac cctctcaatc cctggtaatg aatcaccctt cgtttcttgc agactcctct
    12001 ttctacctgg agaaaccggc agcatgagaa agatgctcaa acctcccccc ggcgccacgc
    12061 ctcgatcagt gcccgagaga tgctcgtttt cgaagggaac ggaggaaggt tctcgcggga
    12121 aaaccagctg gcctcttcga tctcccgggg gtcgatccgg atctctccgc tgtcgtagtc
    12181 tgcgacaaag ccaatcatga gagagtcggg gaacggccag ggctcgctcc cgaaataccg
    12241 gatgtttttc accgcgatgc cgacctcttc gccaacttcg cggtggacag tctgctcgag
    12301 gttctcccct ggttcgttga atcctgcgag gatcgaatag acccctggcg tgaagcgggg
    12361 ggatctggcg agcaggacct tttcgccctt tttcaggagc acgataatgg caggggatac
    12421 ccgggggtag acaatgcggc tgcaggatgt gcagatccgt gcccgctctg tcgtggaagg
    12481 gcgggtattt gcaccgcatt ttccgcagaa tgtattcgag cgatcgaagt caaggatccg
    12541 gatggcatac gcggcatatg ccatgtcgcg gtccggaacc acaccgtgca attccctgac
    12601 cggcgacgtc tcccagccgg gcgggagtgt ggcaccggaa gcaacctctg ctgcataata
    12661 gcaaaggtca ttatcggtgc cgagatacac cggtgcacct gatgcaagtc cttcggggag
    12721 cagatctccc atgaccacgg tgccgggtgc tggctcatcc cggaatacga cggaggtatt
    12781 ctggaccagg atccagcgtc gttgtgcagg cgacaggtcc tccggttccg gaaagaccga
    12841 atggagaggg tggaccgcgt agagtggata gtgctccata tcagatcttc gtgtagcatc
    12901 catggaagtt cttcctggta ctgtctggag tggatttttc acaggaagca ttctggctcc
    12961 tttcgagtac catacagggt gtgcaggcgg accgacgcgt ccctgacgaa ccctggtttt
    13021 atcgtccttg catgcttcgg gcagtgtttg atgcatgcac agcacgtaat gcagtgttcc
    13081 gtgtcgattg ccctgcaatt ctccggatcg atggcaccga ccgggcacac ctctgcgcat
    13141 atcccgcact ggatgcaggc atcactgacc tcgatgaaat cgacattcca gaataccgaa
    13201 tccctccgat agggatgaca aggctcctgc cggtacggac ggcagccggg gattgtgact
    13261 tccgacatcc ggtcgagcga ggatatggac cgaagtttct cccatatgtt cttcccgaac
    13321 acttctgcaa agtcctggtc gttcgcatcc ggacggccca cggcggttgg ggtttcatcg
    13381 gtcgagaaag aatgttcccc aatgaaggct gacccggcga tcgggatgca tccacccttt
    13441 atgaggatgt ccgagagttc gagcagggca tcttcatact cacgattacc atagacgacg
    13501 acacacactg cgggggtgtt ccgggccttt atcacgctca gccattcagt aacgagagcc
    13561 ggcactcttc ccatgtacac cggcacccca ataatgagca gttcgttgtc cgaggcctgc
    13621 aacggctgaa ccctcgcatc cggccgggtg atatcaagct cttcgaagtt gctatggttg
    13681 atcccgcgtg caattgcacg gaccaccgcc tgggtggttc ctgtcggcga gaaatataca
    13741 agtctggctg attcgatgct catggtatga tactccgtca tcaggagagg acgcaggcac
    13801 ggacttgtgc tctgtcgtcg ggcatgatgt tacttttcaa tttacgtctc tccagaattc
    13861 cctgtgttgt gccgggtgtc cgtgtgatgc attccctcgg tgaggattct tcccggaaag
    13921 atgtcacatt caatatatta ctttaagtta ttttataact attggttata gtataaccac
    13981 aagttaaatt tgcggtgatg aaatggaaat gctcatgcct tgtaccagat gtgaacagga
    14041 aatatagatc gaacggtata attcatatca gagtaacagc ccttgcatga aactcattac
    14101 ggtgctcatt accctctgcg tccttgctgg cagcgccgcg gctctctcaa tgccgccggt
    14161 atcgcccctg ttccctccgg cccgcccgga ccttgtgtac gtttcctcgt tcgaaggagg
    14221 ccaccggctc caggcgggcg actacccggt gatcgtcctc tccggcacct ttcggcagat
    14281 gggccggcag tacggcgccc tcatgaaggc cgagttgaac gaggaatacc ggttcatctc
    14341 ctctgcgtac gagaaacgcg gatattcgaa cgaacagctg cgagccgacg gccgggaggc
    14401 gtcagtgctg tactcgaagc ggttgaagga ggtcttttcc gggatggccg agacctcggg
    14461 tctgacccag gatgaagtgc tggccctcta taacgggctt gtcatctatc tcatggtccc
    14521 gcctgcagat gcctcctgct cctacctctc tgcctggggc aactacacga cggacggttc
    14581 tgtcatcgcc tcccgcaact gggacctgga cgatgtcgcc cttcccttca acaagtggaa
    14641 cgtccttgtg gtgtaccgac cctcggacgg cagcaatggg gttgcgacct tcggtcctgc
    14701 aggggtccgg gccgagaccg tcatgaacag caaagggctc ttcatcgctg acgattttgc
    14761 gggcttcatc gatccagcac aggatactag gcctgacctg gtcgcggaat tcttccggtt
    14821 catgctcgat tactcaaatc ttgcggaact ggatgtcggt ctccgggggt cggcgacaaa
    14881 catcggctgg atcgtgaacg tcgccgggcc gggtggtgcc tccgtatacg aggtgacgcc
    14941 caaccagacc cgccagcgga ccggcgacgg cgttgttgcg gccgccaacc actttgtcga
    15001 ccctgcctgg aacctgccgc cacccgcgga gcactcggca tcccgctatg atgggctgct
    15061 ccgggaggct gacgaggcga agggatcgat tgatgcaagg aggatgatgc agatccggga
    15121 cgtccggtgg gaggacggcg gggccacgtt cgtgaactct gtcctctccc cggatcaccc
    15181 cgaggaactc tactccacca tccaccaggt cgtgttcctg ccggaatccc ggacggtctg
    15241 gatcaaggac ccggacagtg cctggcagag ggtcgatctc gggatgttgt tcggggaatg
    15301 aggggaagga agggggattg acccttccca ccttcatgcc tgcttcagcg aagtgaacat
    15361 tgcttgatcc gcaacgagga cactgtggga accacatttg ttgaaagagg aagaaa
//
LOCUS       DAOQYR010000052        15352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_4326_length_15352_cov_8.447866, whole genome shotgun sequence.
ACCESSION   DAOQYR010000052 DAOQYR010000000
VERSION     DAOQYR010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 15352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15352
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4326_length_15352_cov_8.447866"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PKM87_07325"
     CDS             <1..536
                     /locus_tag="PKM87_07325"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HNQ29915.1"
                     /translation="VLLMGIITAEIFYPAGYTTAHSEISDLGATRPPDSISFQPSASI
                     FNTTMIIGGLLILAASLILFWTKTKWYIVLFFALVGTGILGVGLFPGDNVFFHPLFAL
                     LTFISGGLAAIVSFEMTHPPFAYLLALLGVITLFFLFFSPVFIPILGDGGTERFVAYP
                     LIIWMIGLGGYLIGKSG"
     gene            complement(556..807)
                     /locus_tag="PKM87_07330"
     CDS             complement(556..807)
                     /locus_tag="PKM87_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29916.1"
                     /translation="MVGYESACFSLRGRAMKRGMERGSPIVRFLFQQMVAGQWQETKF
                     SIPDHFSSKPGSTVFLKNYMILFETHAYVFMKMAQPSSW"
     gene            827..1780
                     /locus_tag="PKM87_07335"
     CDS             827..1780
                     /locus_tag="PKM87_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNQ29917.1"
                     /translation="MRSHTLPARYFLLLFLIIGAIMGAMILAEIIASEEMGPQVKIII
                     ESPFENTIFEQIFETLAIAILILVLYTIGVYAIVRKMPDEGSRFTAVRIFSAFLIGLG
                     LLLGLMVWVQDPKEIVLVIGIIWGAIVVALRDLIQNMVGSLILLVTRMYRIGDRIHIK
                     GVYGTVMDIGIFRTTLMRLDEESGDHPSGQITTIPNGILFRETVTNTSRDLSFTGDEI
                     RITLPFSADIDKTRMLLLGIVQKHTMEVQEKAMAEIEQLSDRKFLPSFATEPTVFVHL
                     DRHQVLMVVKYYTETRRRSEIKNQIVGEISRVIPGVLDVQR"
     gene            1870..3081
                     /locus_tag="PKM87_07340"
     CDS             1870..3081
                     /locus_tag="PKM87_07340"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNQ29918.1"
                     /translation="MNSETLSLDEALRLVLDHIAPLPPEDVSLVESVGRIAASDLYAL
                     VDSPSLDTSRKDGYAVVSGDVAGATQEHPAWLRVLGVMAAGGAHRIAISPGTAVRVLT
                     GARIPDGASGVVSDEYARKGEDQVAIENSAEPGNILPRGSDVMAGTCILRRGQEISPL
                     MAGLLAVAGHSTISVIRSPVVGILGTGDEITAPGQPLLDGKLYASNIITLAGWCRKYG
                     MTMDLAIVSDDLSAIRTTLQDLSTRTDAILTSGGAWKGDRDLVARVLEEMGWRKVFHR
                     IRIGPGKAAGFGMLNGKPVFVLPGGPPSNANAFLQVALPGLLALSGHPNPGLPRIKAM
                     LAAPLLKGDCEWTDFFFGTLSFDGELPTFVPQKKRSRLATISDATAIASIPEGFDHLP
                     EGSIIDVQVLD"
     gene            complement(3125..3439)
                     /locus_tag="PKM87_07345"
     CDS             complement(3125..3439)
                     /locus_tag="PKM87_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29919.1"
                     /translation="MLNLKIPHAQAIALLEERIEAMKTIRATPDGPEYYDVVGWMSAT
                     HSAIDRVYGGEEIHPEEIRAIGLPACSCSAGRSGRMILEEYRAKLQDYIDEIRRFVSE
                     EG"
     gene            complement(3724..9495)
                     /locus_tag="PKM87_07350"
     CDS             complement(3724..9495)
                     /locus_tag="PKM87_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNQ29920.1"
                     /translation="MKPVFTIAGLFILLLVVSGYAAADGEAWKFRSDLSNSGVYDDGG
                     MRPDGMTVWNYTTENEVYSSPTVVDGVVYIGSDDHSLYAFNAVTGDLIWSYMAGREIR
                     SSPAVADGVVYFGSDDYNIYALDAGTGDLIWNFTTEQVARSSPAVADGVVYVAEGPHK
                     ILALDASNGALLWNSTRSSSPWPVGYVTSSPAIADGMVYVGSYDQNLYAFDTSTGDLA
                     WNFTTADYVFGSPAVFEGVVYFGSWDDNLYALNATNGDLLWNFTTGGAVFSSPAIADN
                     VVYVGSQDHHIYALDAGSGDLLWSSLTGHGVSSSPAVADGVVYIGSNDGNLYALDAVT
                     GTLLWSSPTGNWVFSSPAVANGIVYVGSFDGNLYAIGASPGSSPTPYDDFDDNAWDDT
                     LWNVIESGGPYVTETNQRLEITLPADSSGDNFYAGYSSTHVLRGDFDVQVDYHLLTWP
                     ENNGVRMGLWIEKIPYTYTVERASYGISDGFAAGDHYTTNFADSILLSPTGDTDGKLR
                     LVRTGSLFEGYWRNETSNGWVLSQSRQVNTVSAGEVTFMLKAWSHDYAFADEPVVVAF
                     DNFVINKGTVVIPGITPPEGVTGLANATFQPNLIRWTWEDPGDADFDHVMVYLDGEFL
                     TNVTKGVQAWTAKGLLPSTAYTIGIRTVGEMGAINGTWVNHTATTSSLLVTHLDPPDV
                     LEGSPGFTLDILGSGFGAGSRVLWNGVQEETLFHDGGHLSINVSADMVLRPATVNITV
                     HDPTTGEVSNPVMFRVRDQSSGVRAWKFRSDSSNSGVYDDGGTRPGNELLWKYMTESY
                     YGSSPAVVDGVVYVCGIYDINAIDALTGRLLWTSLNGGGMSSPTVAGNVLYVGSNDYN
                     ISALDASTGAVLWKFRTGSVVYSSPAVVKGIVYAGSWDGNLYALDASTGTLLWNYTTG
                     GEVRSSPAVTDGIVYFGSADRNLYALDAGTGELIWQYVTGDELYYSSPAVADGLVYIQ
                     SSDHNLCALDASTGDVLWTYPVRGWGDSCPAVADGVVYIGSDDGNVYALDAGTGDLIW
                     KYATGNLVRSSPSVANGVVYVGNSAGTVYALDADTGTVLWTYGTNEGGFSSPAIAHGV
                     LYIQGLNGYLYALTTLPDDPPASVTDLYAITVNGAEITWAWTDPATLGFSHVMVYLDG
                     MFRENVSAGEETWTSKGLTPSTSYTIGIKTAGVNGLVNATLVTGTATTGSLSISALDP
                     VSVVEDDPAFTLDVHGTGFTPSCSILWNGVEQSTSCIQPDHLSMEVPEEYVAHSGRVN
                     ITVHDSVSGESSNAVILRVTDNPATAMARKFRSDLNNSGVYDDSGRRPVPSLLWTYKT
                     GGSVTSSPSLVDGVVYVGSLDRNLYALNATTGAFLWQYNTMERNDYVSSSPAVSNGVV
                     YIGGLKNKIHAVDAYSGELLWKYTLPIRSTVRSSVSSSAAVADGIIYIGNMDGTVYAF
                     DEEDGGLLWSYAIPRLEYDEHNIFSSPAVADGIVYVQTYGENLYALDASTGALLWTSL
                     NENGWSAFSSPAVSEGVVYVGGGRENAFHAFDAQTGSVLWEFPTGGSIGSSPALANGI
                     VYFGCNDNIIYALDVITGAQIWNFTTGGRVQSSPAVANGVIYIGSYDNNTYALDASTG
                     DLLWSFDVGGRVTSSPAVADGVVYIGSGDGNVYAIGTLPLNPPVTDFSANVTSGDAPL
                     DVEFTDTSTGVVTTRYWDFGDGNTAWANETLAVTHTYTFPGTFTVSLTAGNIDAQATK
                     TKTDYIQVSPSGRPPAAWFTASPMSGYGPLAVRFTDRTKGTPVAWQWDFGDGNTSTEQ
                     NPVHTYATPGTYTPMLTAFNSGGSSAYTSFVWVRTKPVIPTFTPTPTITPRPTIPPIP
                     GFPPIAFFAMNKSVGSAPLTVQFNDMSFRGPDSWEWNFGDGETSIMRNPVHTFAHPGT
                     YPVSLSVENANGRSTTSRNVYVR"
     gene            9631..11016
                     /locus_tag="PKM87_07355"
     CDS             9631..11016
                     /locus_tag="PKM87_07355"
                     /inference="COORDINATES: protein motif:HMM:NF020416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HNQ29921.1"
                     /translation="MPSCFTCPVLLSGTAVLILVVFLVAAGCIATPQAPSLSVTPAVV
                     LSGDPVTITITGIAPGERVRVEAVQEVRNATLHSYAVFVADGQGRVLPDVHAPVDGTY
                     GGVDPQGLFWSLAPGPGEHPDVFSRTTAPSFITISARTESGVNLSRVLERRLMGDGVT
                     RVPVNEAGVRGTLFLPNGSDPHPALIYLGGSEGGVNEGIAAIFASKGYVTLALAYFGV
                     PGLPQELVGIPVENVGDAVRFLNHHPRVKEDHIAIYGASKGAELALLSATRYPEIRAV
                     IANSPASVVFQGISMDWSAGPRSSWSENGSDLPYVPFIWYGDDDPILVSMGEAAQNGT
                     PWGTLAMYERALGDEAAVSNATIPVERINGPVLLLSAGKDTVWPSSRMSGDIMARLAE
                     YRHPFPDMRLDYPGSGHMIRTPWQSTELNRIFLPGGMIEDLGGIPPENAKAAADSWPK
                     VQEFLRVALGS"
     gene            complement(11212..12465)
                     /locus_tag="PKM87_07360"
     CDS             complement(11212..12465)
                     /locus_tag="PKM87_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29922.1"
                     /translation="MVPMTRSCLVFTVFAVLVIVQGVSPASVSISPDQVMQGDLVTIT
                     IGDLSDACAFSIRIDGTFAAAQGNPFSFGVRDFLLPFTLNDGSISATLWNTETNMMTI
                     RKGDTEVRRVGLSQDGVFSTATTGTIPGGTYDLISLGGEAAPGATNIVASLSIEGSKS
                     GPDDSAITFFVEGVTDGNVTIAVLVDGETELFRTLPIGNPITITPTPTTPAPTPTTPT
                     PTPTTPTPTPTTPDLTPTPPAPTPTPAGNGIPLEPGWNFVSVPRTLAEGHDVVDAVFT
                     GVDTGGRSIFTYNGQDQAWVQLRYGDTVRLLDGIWIYSADERDLSLVYAASGVPGTMT
                     KQLFSGWNTIGFSSTESMEVRYALASVNDTWFQVYGYDSALQRYEPAILKNGIGTHSD
                     TRHFHPFMGYWVKMNGNGTLFAIGN"
     gene            12713..14050
                     /locus_tag="PKM87_07365"
     CDS             12713..14050
                     /locus_tag="PKM87_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ29923.1"
                     /translation="MDRTTLLGICLLVIVITAGIAASGATNIQTTVTKPTGDTIEKMQ
                     KVQDEATARKNQPGGYPSPSTGGKYHTRLVLLGTTGGVTWYPGTDRASSSSALVVGDT
                     LYIIDLGQGSTSRLSEAFNPGHPLVGQEMGSTSTFLEKARALFFSHLHQDHIADYPSL
                     LLIGPGAGLGTRTDPATGVVSIVPLVVIGPADRGMCEIDRSHYLDRGGTIVYTDSADP
                     ALVTPTPGTRLMTSLIWQAYAQTINDMTLDNAYRDFTKLVEVREIGGTGPGDISFPVP
                     EVDLANGTTCAPMDPFEVYRDENVVVTAALVDHHQVFPTFAYRFDTDDGSVVFSGDTG
                     PDTRGNLQKLGEGADILVHEVIDRAWIDQRMGFPDEDTPAYALKTHLLTSHTTIDMVG
                     SVARDCRVDTLVLNHIVPGNTPTSHLLQAQQNFSGRLIIGEDLMEIGVGKPVR"
     gene            complement(14151..14519)
                     /locus_tag="PKM87_07370"
     CDS             complement(14151..14519)
                     /locus_tag="PKM87_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29924.1"
                     /translation="MSERPLGVSVLGVLWIFIACIWFVAAILGVAVLAGLGFPGLGAI
                     AGVILFCIAIVDLLLGIGCFRGWGIVWTVGVLMMVINIIFGLMTLFSAWIFGVLSIIV
                     SAIILWYFFQPQVKKYFGKA"
     gene            complement(14765..15325)
                     /locus_tag="PKM87_07375"
     CDS             complement(14765..15325)
                     /locus_tag="PKM87_07375"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNQ29925.1"
                     /translation="MEKITLGPMPYMSVMPTVLVGANVQGRPNYMVAAWATVACMAPP
                     MVCVTINKGRYTWKGIEENRTFSLNVPSVSQVVETDHCGLVSGAREDKSGVFRSVYGT
                     LGTAPMAAECPVNIECELFESVDCGSHLLVIGKVAEIHAAKSCVTDGVPDIRKIDPII
                     YAQHAYYRVGDDAGEAFSAGKKYRKG"
BASE COUNT         3184 a         4624 c         4352 g         3192 t
ORIGIN      
        1 cagttctcct gatgggcatc atcaccgcgg agatattcta tccggccgga tacacaactg
       61 cacatagcga gatcagcgac cttggggcga cccggcctcc tgacagcatc tccttccagc
      121 cttcggcatc gatcttcaat accacaatga tcatcggcgg cctcctcatc ctcgcagcgt
      181 ccctcatcct cttttggacg aaaacgaagt ggtacatcgt cctctttttc gcactggtcg
      241 gaaccggcat cctcggggtg ggattgttcc cgggtgacaa tgttttcttt catcctctct
      301 tcgcactcct caccttcatc tccggaggtc ttgcggcaat tgtctccttt gagatgacgc
      361 atccaccgtt tgcttacctg ctcgcccttt taggggtcat taccctcttt ttcctgttct
      421 tttcaccggt attcatcccc atccttggtg atggtggaac ggagcgcttt gttgcgtatc
      481 ccctcatcat ctggatgatt gggcttggcg ggtaccttat cggaaaatcg ggttaggtga
      541 taataatccc attttttacc atgaagatgg ctgggccatt ttcataaata cgtatgcgtg
      601 tgtctcgaag agaatcatgt agttttttaa aaatacagtc gatcctggct ttgaactgaa
      661 gtgatccggg attgagaatt tggtctcctg ccactggccc gccaccatct gttgaaataa
      721 aaagcggaca attggagatc ccctctccat acctctcttc attgcccgac cgcggagaga
      781 gaaacaggca gattcatagc caaccatgga ataacccctc atcaagatgc gatcacacac
      841 cctcccggca cggtatttcc tgcttctctt cctgatcatt ggggcgataa tgggggcgat
      901 gatccttgct gagatcatcg catccgaaga gatgggtcct caagtgaaga ttatcatcga
      961 gagcccattt gaaaatacca tcttcgaaca gatcttcgag acgctcgcca tcgccatcct
     1021 catcctcgtc ctgtatacca tcggcgtcta cgccatcgtc aggaagatgc cggacgaagg
     1081 gtcgcggttc accgccgtca ggatcttctc tgcattcctt atagggctcg gactcctgct
     1141 cggcctgatg gtatgggtcc aggacccaaa ggagatagtc ctcgtgatcg gcatcatctg
     1201 gggagcaatc gtcgtcgcac tccgcgacct catccagaac atggtgggaa gccttatcct
     1261 tcttgtcacc cggatgtacc ggataggcga ccggatccac atcaagggtg tctatgggac
     1321 tgtcatggac atcgggatct tccggaccac cctgatgcgg ctcgacgagg agtccggcga
     1381 tcaccccagc ggccagatca ccaccatccc aaacggcata ctctttcggg agacggtcac
     1441 caatacgagc cgcgacctct ccttcactgg cgacgagatc cgaatcacgc tcccgttctc
     1501 tgccgatatc gataaaaccc ggatgcttct cctcgggatc gtgcagaagc ataccatgga
     1561 ggtccaggag aaggccatgg cggagatcga acaactcagc gacaggaaat tccttccgtc
     1621 gtttgcgacc gaaccgacag tcttcgtgca ccttgaccgg caccaggtcc tgatggtcgt
     1681 caagtattat acagagaccc ggaggagatc tgagatcaag aaccagatcg tcggggagat
     1741 ctcgcgggtc atcccgggcg tcctggacgt ccagcggtag ggtgacccgg atcgcatcac
     1801 attatgaagg agaaaggagc agtctctgct gtgcaaaagg atcggagaaa aagagggagg
     1861 agatattgaa tgaacagcga aacgctcagc ctggatgagg cgttgcggct tgtactggac
     1921 cacatcgcgc cgcttccccc agaagacgtt tcgcttgtcg agagcgtcgg gcgtatcgcc
     1981 gcatcagacc tttatgccct cgtcgactct ccatcgctgg acacatcccg aaaagacggc
     2041 tacgcagtcg tatcgggcga cgtggccggt gcaacgcagg agcaccccgc atggctcaga
     2101 gtgctcggag tcatggcggc cgggggcgca catcgcattg ctatctcacc aggtaccgcg
     2161 gtgagggtcc tgaccggggc acgtatcccg gacggggcaa gcggcgtcgt ctccgatgag
     2221 tatgcgagga agggtgagga ccaggtggcg atagagaact ctgcagaacc cgggaatatc
     2281 ctgcccaggg gaagcgacgt tatggcaggg acctgtatcc tcaggagagg ccaggagatc
     2341 tcccccctca tggcaggcct ccttgccgtt gcagggcaca gcaccatttc cgtcatccgg
     2401 agccctgttg tcgggatcct tgggaccggc gatgagatca ctgccccggg ccagcctctc
     2461 ctggatggaa aactctatgc aagcaacatc atcactctcg ccgggtggtg caggaaatac
     2521 gggatgacga tggatctcgc aattgtatcg gatgatctct ctgccatccg caccactctg
     2581 caggacctgt ccacacgcac cgatgccatc ctcaccagcg ggggggcctg gaagggtgac
     2641 cgggacctcg tggcccgcgt gctcgaagag atggggtgga gaaaggtgtt ccaccggatc
     2701 cgcatcgggc cgggaaaagc cgcggggttt gggatgctca acggaaaacc agtctttgtg
     2761 ctgcccggcg gaccaccctc caatgcgaat gccttccttc aggtcgcatt gccgggtctc
     2821 ctcgcacttt ctggacatcc gaaccccgga ctgcccagga taaaggcaat gcttgccgct
     2881 ccgctcctaa agggtgattg tgagtggacc gatttcttct ttggcaccct ctcgttcgat
     2941 ggtgagcttc cgactttcgt tccacaaaaa aaacgcagtc gccttgcgac gatctcagat
     3001 gccacagcaa tcgcatcgat ccccgagggg tttgaccacc tcccggaggg ctcgatcatc
     3061 gatgtgcagg tgctcgacta gagaacgccg tccgccagcg gctgggggtg tttaggcctc
     3121 ccgctcatcc ttcttccgac acgaatctcc tgatctcatc gatgtaatcc tgcagttttg
     3181 cgcgatattc ttcgagtatc atccggccgc tcctcccggc actgcaggaa caggcaggaa
     3241 gcccgattgc ccggatctct tcagggtgga tctcctcccc cccgtacacc cggtcaatcg
     3301 cggaatgagt cgctgacatc cagccgacca catcgtaata ttcagggccg tccggtgtgg
     3361 ccctgatagt cttcatggcc tcgattcgtt cctcgaggag ggcgatggcc tgtgcatggg
     3421 gaatcttgag attgagcatt cctgtatcaa tccttggaca ggagttcagt ggggaaagtg
     3481 aaaaaagtta tgaggccgta ggaggagtga aatgcaccat tccgggtgag actgcagtaa
     3541 ataatcctcc tgatacctgt gatttgcccg ggatgcctga ttgttatact gattcggtga
     3601 aaagtagtcc gggagagagt tgggagggat caaagacaat tatttgaccc tgtaagacac
     3661 gccacaatat cccgcacagg gagttacggg tttcaggttc tgcggaaaaa atgatttact
     3721 cctctaccgc acgtacacgt tccgggacgt ggtgctcctt ccgttggcgt tctctaccga
     3781 gagtgatacc gggtaggtcc cggggtgcgc aaaggtgtgg acaggatttc tcattatcga
     3841 ggtctccccg tcgccaaagt tccactccca ggagtccgga cctcggaacg acatgtcgtt
     3901 gaactggacc gtcaggggag cggacccgac acttttattc atcgcaaaga acgcgatcgg
     3961 ggggaacccg gggatcgggg ggatcgtggg tcttggggtg atggtcgggg tgggggtgaa
     4021 cgtcgggatc acgggttttg ttcgtaccca cacgaacgat gtgtatgcac tgctcccgcc
     4081 agaattgaag gcggtgagca tcggggtgta tgtcccaggg gtagcgtagg tatgcaccgg
     4141 gttctgctcg gtcgaggtgt ttccatcccc gaagtcccat tgccatgcca cgggcgtccc
     4201 ctttgtccgg tcggtgaacc tgaccgcgag cggtccatat cccgacatcg gggatgcagt
     4261 gaaccacgcc gcaggtggcc tgcccgacgg gctgacctgg atgtaatccg tctttgtctt
     4321 ggtggcctgt gcatcgatgt tcccggcggt cagcgagacc gtgaaggtcc ctgggaaggt
     4381 atacgtgtgg gtgaccgcca gcgtctcgtt tgcccatgcc gtgttcccgt cgccaaagtc
     4441 ccagtaccgc gtcgttacga caccggtcga agtgtcggtg aactccacgt cgagcggggc
     4501 atcgccggaa gtcacgttgg ctgaaaagtc agtgacaggg ggattaagag ggagcgttcc
     4561 tatcgcatac acgttgccgt cgccgctgcc gatatagacc actccatcgg ctaccgcagg
     4621 actggacgtg actcttcccc ctacatcaaa ggaccagagg aggtctccag ttgatgcatc
     4681 gagtgcatag gtattgttgt cataacttcc gatatagatc acgccgttcg ccactgccgg
     4741 gctggactgc acccttcctc ctgtcgtgaa attccagatt tgtgcaccag tgataacgtc
     4801 gagtgcgtag atgatgttgt cattgcaccc gaaatacacg attccgttcg cgagggcagg
     4861 gctggaaccg atactgccgc cggtcgggaa ttcccagagc acactccctg tttgtgcatc
     4921 aaatgcatgg aatgcatttt ccctgccgcc cccgacatag acgacccctt ctgataccgc
     4981 ggggcttgaa aacgcagacc agccattttc gttcagggag gtccagagaa gagccccggt
     5041 ggatgcgtca agtgcgtaga ggttctcccc atatgtctgc acgtatacga taccgtctgc
     5101 aaccgccggg ctggaaaaga tgttgtgttc atcatattcc aaccggggga tggcatagct
     5161 ccagaggagg cccccgtcct cttcatcgaa cgcgtagacg gttccatcca tgttaccgat
     5221 atagatgatc ccatctgcca ctgctgcact ggagctcacg ctgctcctca ctgtggagcg
     5281 aatcggcagt gtgtacttcc agaggagttc gccggaatac gcatcgaccg cgtggatttt
     5341 gtttttcagg ccaccgatgt agacgactcc gttcgagact gcagggcttg aactcacgta
     5401 gtcgttgcgt tccatggtat tatactgcca gaggaatgct ccagtcgttg cgttcaacgc
     5461 gtagaggttc ctgtccaggc tccccacgta cacgacgccg tccactaatg atgggcttga
     5521 cgtcaccgag cctccggtct tgtaggtcca gaggagcgaa ggcaccggcc tcctcccact
     5581 gtcatcatac accccggaat tgttgagatc ggatcggaac ttcctcgcca tcgcggtcgc
     5641 cgggttgtcg gtcacacgca ggatcacggc gttcgatgat tccccggata cggaatcgtg
     5701 cacggtgatg ttcacgcgtc ccgagtgggc gacatactct tccgggactt ccatgctcag
     5761 gtggtccggc tggatacagg aagtggactg ttctactcca ttccagagga tactgcagga
     5821 tggggtgaac cccgtgccat ggacatcgag cgtgaatgca gggtcatcct ccaccacact
     5881 cacgggatcg agggcagaga tcgagagtga tccggtggtc gccgttccgg tcacgagggt
     5941 cgcgttgacc agcccattca ccccggcagt ctttattcca atcgtgtagg aggtcgaggg
     6001 agtgagcccc tttgaagtcc aggtctcctc accggccgat acgttctccc gaaacatccc
     6061 atcaaggtaa accatgacat gggagaaccc gagcgttgca ggatcggtcc aggcccacgt
     6121 gatctctgcc ccgttcactg tgatggcata caggtcggtc acgctcgctg gcgggtcgtc
     6181 agggagcgtg gtaagcgcgt ataggtaccc gttcaacccc tggatataca ggaccccatg
     6241 tgcaatggca gggctcgaga atcccccttc attcgtacca tacgtccaga ggaccgttcc
     6301 ggtgtctgca tcgagcgcgt agactgttcc ggcagaattc ccgacatata cgacaccgtt
     6361 tgccaccgag gggctcgatc gtaccaggtt ccctgttgca tacttccaga tgaggtcccc
     6421 ggtccctgca tcgagagcat aaacattgcc gtcatcgctc ccgatataga ccaccccgtc
     6481 tgccactgca gggcaggagt ctccccaccc tctcacagga taggtccaga ggacatcccc
     6541 ggtggacgcg tcaagcgcgc agaggttgtg gtcactgctc tggatataga cgagaccgtc
     6601 tgcgactgcg ggagatgagt agtacagttc atctccggtc acatattgcc agatgagttc
     6661 tcccgtcccc gcatcgagtg catagaggtt acgatcagcg ctcccgaagt agacgatccc
     6721 gtccgtcacc gcggggctcg atcgcacctc gccgcctgtc gtatagttcc agaggagagt
     6781 gccggtggac gcgtcgagcg catagaggtt cccatcccag ctcccggcat acacgatgcc
     6841 tttgaccacc gcggggcttg aatacacgac cgacccggtc cggaacttcc agaggacagc
     6901 tccggtggag gcgtccagtg cggaaatgtt atagtcgttg cttcccacgt agaggacatt
     6961 gcccgcgacc gtggggctgg acatcccccc tccgttcagg gaggtccaga gaagtctccc
     7021 ggtgagcgcg tcaatggcgt tgatatcgta tatcccgcag acatagacga ctccatccac
     7081 gaccgccggg ctggaaccgt aatagctctc tgtcatgtac ttccagagca gttcgttccc
     7141 gggcctcgtc cctccgtcat cgtagacgcc ggagttggag gagtcggacc ggaacttcca
     7201 ggcccggaca ccagaggact ggtctctcac gcggaacatc accgggttcg agacctcgcc
     7261 cgtcgtgggg tcatgcaccg tgatgttcac ggtagccggc cgcagaacca tatctgccga
     7321 cacattgatc gagaggtggc ctccatcatg gaacaaagtc tcctcctgaa cgccattcca
     7381 gaggacacgg gagcctgctc caaatcctga tccgaggata tcaagggtaa agccggggct
     7441 cccctccaag acatccggcg ggtcgaggtg cgtgaccagg aggctgctcg tggtggcggt
     7501 gtggttcacc caggtcccgt tgatcgcgcc catctccccg acggtccgta tcccgatggt
     7561 ataggcagtc gaggggagga gccccttcgc ggtccaggcc tgcacaccct tcgttacgtt
     7621 tgtcaggaac tccccgtcca ggtagaccat gacgtggtcg aaatctgcat cgccggggtc
     7681 ctcccaggtc cagcggatga ggttcggctg gaaggtcgcg ttcgcaagac cagtgactcc
     7741 ttcgggggga gtgattcccg gaatgacgac tgtccccttg ttgatcacga aattgtcgaa
     7801 tgcaaccact accggttcat ccgcgaaggc atagtcatgg ctccatgcct tcagcatgaa
     7861 cgtcacttcc cctgcagaca cggtgttgac ctggcggctc tgcgagagca cccatccatt
     7921 ggaagtttcg ttccgccagt acccctcaaa gagggagcct gtccgcacca gccgcagttt
     7981 cccgtccgta tcgcctgtcg gggagaggag gatcgagtca gcaaaattcg tcgtgtagtg
     8041 atcccctgcg gcaaaaccat ccgagatccc gtaacttgca cgctcgacag tgtaagtata
     8101 aggaatcttt tcaatccaga gtcccatcct cacgccgttg ttctctggcc aggtgaggag
     8161 gtggtagtcg acctggacat caaaatcacc ccgaaggacg tgtgtgctcg aataccctgc
     8221 atagaagttg tcgccggatg aatcagcggg gagggtgatc tccagccgct ggtttgtctc
     8281 ggtgacatac ggaccccctg attcgatgac attccagagg gtgtcatccc atgcattgtc
     8341 atcgaaatcg tcgtatggcg tagggctgct cccgggagac gcaccgattg catacaggtt
     8401 cccgtcaaag cttccaacat agacgatgcc gttcgcaacc gcggggcttg aaaacaccca
     8461 gttgcctgtc ggggagctcc agaggagagt gccggttact gcatccaggg catagaggtt
     8521 cccatcgtta ctcccgatat agactacgcc gtccgcaacc gcgggactcg aagagacgcc
     8581 atgccctgtc agggagctcc agagaaggtc accacttcct gcgtcgagcg catagatgtg
     8641 atggtcctga ctcccgacat agacgacgtt gtctgcaatg gcagggcttg agaagactgc
     8701 accgcccgtg gtaaagttcc agaggagatc gccattcgtt gcattgagtg catacaggtt
     8761 gtcatcccaa ctcccaaaat acaccacccc ttcgaatacg gcagggcttc cgaatacata
     8821 gtccgcggtc gtaaagttcc aggcaagatc acctgtcgat gtatcgaatg catagaggtt
     8881 ctgatcataa cttccaacat acaccatgcc atctgcgata gcagggctgg atgtcacata
     8941 gccgacaggc catggggagg agcttctggt agaattccag aggagtgccc cattggatgc
     9001 atcgagtgca agaatcttgt gcggaccctc cgcaacatag acaaccccat cagccacggc
     9061 cggactggac cgtgccactt gctccgttgt aaaattccag atgaggtccc cggtcccggc
     9121 gtcgagggcg tagatattgt aatcatcact cccaaaatat accacaccat ctgccaccgc
     9181 aggactcgac cttatttccc gcccggccat atacgaccag atgagatctc cagtgaccgc
     9241 attgaacgca tagagggagt ggtcatcgct cccgatatat accactccgt ctaccactgt
     9301 tgggctggaa tacacttcat tttctgtcgt gtagttccat accgtcatcc cgtcaggcct
     9361 catcccccca tcgtcataga ctccggagtt ggagaggtcg gaccggaact tccaggcctc
     9421 cccgtcggct gccgcgtatc cggataccac aaggagaaga atgaacagtc ctgctattgt
     9481 gaaaactggt ttcatgggat cactaccgat atgtcgtaaa agaacaattg gcggacacta
     9541 tttatgtatg tgcgcatccc gtggcaagag ggcacgcctg aacccccaga ccgcacacct
     9601 ttatcccctc taccatgaaa acacgagaat atgccatcat gcttcacctg ccctgtgctg
     9661 ctctccggga cggccgtatt gatactcgtg gtctttctcg ttgccgcagg atgcatcgcc
     9721 accccgcagg caccctcact ctcggtcacc cccgcagtcg tcctgtccgg agacccggtg
     9781 acgatcacga taaccggcat tgctcctggc gagcgggtcc gggtcgaggc agtccaggag
     9841 gtcaggaacg cgacgctcca ctcttacgcg gtctttgttg ctgacgggca gggcagggtc
     9901 ctccccgatg tccatgcccc ggtggacggg acctatggcg gggtcgatcc ccaggggctc
     9961 ttctggtcac tggccccggg tcccggcgag cacccggacg tcttctcgcg gaccaccgcc
    10021 ccctccttca tcacgatctc ggcacgcacc gaatccggag tgaacctctc ccgggtcctg
    10081 gaacgccggc tgatgggtga cggggtcacc cgggttccag tcaacgaggc cggggtgcgt
    10141 ggaaccctct tcctgccaaa cggttcggat ccccacccgg cactcattta cctcgggggc
    10201 tctgagggag gagtaaacga aggaatcgcg gccatcttcg cgtcgaaagg atatgtcacc
    10261 ctcgccctcg cctacttcgg ggtccccggt cttccgcagg aactggtggg aatcccggtc
    10321 gagaatgtcg gagacgcggt ccggttcctg aaccaccacc ctcgcgtgaa ggaggaccat
    10381 atcgcgatct acggcgcctc caagggggct gagctcgccc tcctctccgc cacccgctac
    10441 ccggaaatcc gtgcagtgat tgcaaacagc cccgcaagcg tcgtcttcca ggggatctcg
    10501 atggactgga gtgccggccc ccgatcctcc tggtccgaga acgggagcga cctcccctat
    10561 gtcccgttca tatggtacgg ggacgatgac cccatcctcg tctcgatggg agaagcggca
    10621 cagaacggga ccccctgggg taccctcgcg atgtatgagc gggcgctggg cgacgaggcc
    10681 gcggtgagca acgcgacgat cccggtggag cggatcaacg ggccggtcct ccttctctcg
    10741 gccgggaagg acacggtctg gccgtcatcg cggatgagcg gggacatcat ggccaggctc
    10801 gccgaatacc gccacccgtt cccggatatg cgcctcgact acccagggtc ggggcatatg
    10861 atacggacgc catggcagtc gaccgagctg aacaggattt tcctgccggg aggaatgatc
    10921 gaggacctcg ggggcatccc gcctgagaac gcaaaagccg ccgcggactc ctggccaaag
    10981 gtccaggagt tcctgagagt ggcgctcggg tcttgagaga gtctcacaac gcagcgggga
    11041 tttcccccgc aaccccgggt ttctttgggg ggcatggagc gaagggacgc cttccaggga
    11101 gggtcaaaag ggtcgttgca tatcgccccc tgatccggaa aagagttggt ggcgcaccgg
    11161 acccgggaac tcctgcatca tcccgcgaca cataatccaa cgagccgtta tttagttccc
    11221 gatggcaaaa agcgtcccgt ttccgttcat cttcacccag taccccatga aaggatggaa
    11281 gtgccgggtg tcgctgtggg tcccaatccc gttcttcaga atcgccggct cgtacctctg
    11341 gagggctgaa tcatacccgt agacctggaa ccaggtgtca ttgactgacg cgagggcata
    11401 ccggacctcc atcgattccg tggaggagaa accgatagtg ttccacccgg aaaagagttg
    11461 cttcgtcatc gtgcccggca ccccgctcgc agcgtagacg agagagagat ccctttcatc
    11521 cgccgagtaa atccagatcc catccagcag ccgcacggta tccccatacc gcaactgtac
    11581 ccaggcctgg tcctgcccgt tgtacgtgaa gatggagcgg ccgccggtgt cgacccctgt
    11641 aaacaccgcg tcgaccacgt catgcccttc cgcgagtgtc ctcgggaccg agacgaagtt
    11701 ccaccctggt tcgaggggga ttccatttcc tgcaggcgtt ggagtggggg cagggggggt
    11761 tggggtgaga tctggagtag tcggtgttgg cgtgggagtg gttggagtag gggtcggggt
    11821 tgtcggcgtc ggggcagggg tggtgggtgt gggggtgatc gtgatcgggt ttccgatggg
    11881 aagggtacgg aagagctccg tctccccgtc gacaaggacc gcgatcgtga cattcccgtc
    11941 agtcaccccc tcgacgaaga acgtgatcgc cgagtcatcc gggccggact tgctcccctc
    12001 aatcgagagg gaggccacga tgttggtggc tcccggtgcc gcctcacccc caagggagat
    12061 gagatcgtag gtccctcccg gtatggtgcc agtcgtggcg gttgagaaca ccccgtcctg
    12121 ggagagtcct actctcctca cctcggtgtc acccttcctg atcgtcatca tgttggtctc
    12181 ggtgttccac agcgtcgcag agatggagcc gtcgtttaag gtaaatggga ggagaaagtc
    12241 ccttaccccg aacgagaacg ggttcccctg tgccgccgca aaagtgccgt cgatccggat
    12301 tgagaaagcg caggcgtccg aaaggtcccc gatcgtgatg gtcaccaggt ctccctgcat
    12361 aacctggtca ggggagatgg aaaccgatgc cggggagacc ccctgcacga tgaccagcac
    12421 tgcaaacacc gtgaagacaa ggcaggaacg ggtcattggc accatggaat tctccccccg
    12481 ggattcatca caatcagatg gtccatactc cttattagga attcggtgcc ggccgggacg
    12541 cttcgggtct tttaaaccct gcctgtagtg ccgttcaatc ccctttcccc ggcctctcca
    12601 gggagaatca cgcggagcac atgcggacct gaaggatatg tccttctcca acgggaaacc
    12661 tttaccacct ttttcgagag attcctattc attcaggaag tggctctttt ccatggatag
    12721 aacaacactc ttgggaatct gcctcctggt catcgtgatc acggcaggca ttgcggcatc
    12781 gggggcaacg aacatccaga cgactgtcac aaaaccgacg ggtgacacga tcgaaaaaat
    12841 gcagaaggtc caggatgagg cgaccgccag aaagaaccag ccgggcgggt atccttctcc
    12901 ctccacggga ggaaagtacc acacgcggct cgtcctcctg gggacgaccg ggggcgtgac
    12961 atggtacccg ggaacggacc gtgcgagttc ctcgtcagcc ctcgtggtcg gcgacaccct
    13021 ctatatcatc gatctcgggc agggctcgac ctcccggctc tcggaggcct tcaacccggg
    13081 ccatccactg gtcggccagg agatgggctc cacctctacc ttcctcgaga aggcgcgggc
    13141 actcttcttt tcgcacctcc accaggacca tatcgcggac tatccttccc tcctcctgat
    13201 cggcccgggg gcgggcctcg gcacccggac tgatcctgcc accggagtgg tatcgatcgt
    13261 gccccttgtg gtcatcggcc ctgccgaccg gggaatgtgt gagatagaca ggtcgcatta
    13321 ccttgacaga gggggcacga tcgtctacac ggacagcgct gaccccgccc ttgtcacccc
    13381 cacccccggg accaggctga tgacgagcct gatctggcag gcctatgccc agaccatcaa
    13441 cgacatgaca ctcgacaacg cctaccgcga tttcacgaaa ctggtcgaag tcagggaaat
    13501 cggcggcacc gggcccggcg atatctcatt ccctgttccc gaagtggatc tcgccaatgg
    13561 gacaacctgc gcaccaatgg acccgttcga agtgtacagg gatgagaacg tcgtggtgac
    13621 cgcggccctc gtcgaccacc accaggtgtt cccgacgttt gcctaccggt tcgacaccga
    13681 tgatgggtcc gtggtcttct cgggcgacac cggccctgat acccgaggca atctccagaa
    13741 gctcggcgaa ggggccgaca tcctcgtcca cgaggtcatc gaccgtgcct ggatcgacca
    13801 gaggatggga ttccccgacg aggacacccc tgcctacgcg ttaaagacgc atttgctgac
    13861 ctcccacacg acgatcgaca tggtcggatc ggtcgcaaga gactgccgcg ttgacaccct
    13921 cgtgctcaac cacatcgtgc ccggcaacac tcccacgtct cacctcctgc aggcccagca
    13981 gaacttttcc gggcgcctga taatcggtga ggacttgatg gagatcgggg tgggaaagcc
    14041 ggtccggtga tcccccgggc tttccgatac aatttttttg tgtgggacta tctgaagaaa
    14101 ctatgaaaag gggccggatc cctctgtcca cttaaaataa aggagaattt tcacgccttc
    14161 ccgaagtact tcttcacctg gggttggaag aagtaccaga ggatgatcgc cgatactatg
    14221 atgctgagca cgccgaaaat ccaggccgag aacagggtca tgagcccgaa tatgatgttg
    14281 atgaccatca tgaggacgcc gacggtccag acaatgcccc atcccctgaa acacccgatt
    14341 ccaagcagga ggtcgacgat tgcaatgcag aagagtatca ctccagcaat cgcaccaagt
    14401 ccgggaaagc caagtcctgc cagcacggcg acccccagga tggcggcgac gaaccatatg
    14461 catgcgatga agatccagag gactccgaga acggaaactc ccaacggtct ttctgacata
    14521 cggaatgaat ctcagaggga agtataaatg aattgtgcac gcctcgccgg gtccacccaa
    14581 cgcatccccg gactcctccg cactcactca tgctccccac ccgggcaagc gtgcggtcac
    14641 gggggaaccc ccatatcata tgcacgatga gccgctgctc gttgactccc catcccggat
    14701 aagaattccc cggattcttt caaggcagtt gtgggtcatc ctgcctgttc caggaactcg
    14761 aaaatcaccc cttcctgtac ttcttcccgg ctgaaaacgc ctctcccgca tcgtccccca
    14821 cgcggtagta ggcgtgctgc gcgtagatga tcgggtcgat cttccggatg tccggtaccc
    14881 cgtcggtcac gcaggacttc gcggcatgga tctccgcgac cttcccgatg accagcaggt
    14941 ggctcccgca gtccactgac tcgaacagtt cgcactcgat gttgaccggg cactccgcgg
    15001 ccatcggtgc agtcccgagt gtcccgtata ccgatcggaa caccccggac ttgtcctccc
    15061 gggcaccgga gacgaggccg cagtggtcgg tctcgacaac ctgggatacc gagggcacgt
    15121 tcagcgagaa ggtcctgttc tcctcgattc ccttccaggt ataccgcccc ttgttgatcg
    15181 taacgcagac catcggcggc gccatgcacg cgacagtcgc ccaggcagcg accatgtagt
    15241 tgggccttcc ctgaacattt gcacccacga gcaccgtggg catgacactc atatacggca
    15301 tcgggccaag cgttattttt tccatgcgct cctccatcgg gtatgcgaat ca
//
LOCUS       DAOQYR010000053        14645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_4575_length_14645_cov_8.403153, whole genome shotgun sequence.
ACCESSION   DAOQYR010000053 DAOQYR010000000
VERSION     DAOQYR010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 14645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14645
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4575_length_14645_cov_8.403153"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..907
                     /locus_tag="PKM87_07380"
     CDS             59..907
                     /locus_tag="PKM87_07380"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNQ29926.1"
                     /translation="MLVVSTEYSQILAANVANALECPLGGVRFTRFPDGEHYLRMTAT
                     EREMVVVGSVPDSDALVQLLLLVDACDGAEVTLVVPYMGYARQDKQFHAGEPLSARAI
                     ARVLSTGVSRVFTVNVHDRAVLSHFCVPAHDLSLAADIGRYLSTKSLTRPLVLAPDDG
                     AAAFAHRVASVCGWESDHLEKTRLSGAEVKMEPRRLEANGRDVVIVDDIISTGGTLAT
                     AAAMLMEQGAQSVTSVCVHGVLAEGAYARLLSGSIREVACSDTLERACSRYSAGRAIA
                     DGIRRC"
     gene            901..1899
                     /gene="rtcA"
                     /locus_tag="PKM87_07385"
     CDS             901..1899
                     /gene="rtcA"
                     /locus_tag="PKM87_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HNQ29927.1"
                     /translation="MLSIDGASLEGGGQIVRAAVALSALTGTPIRIERVRAGREQPGL
                     RPQHVAAVRAVGAACSAEVIGCLPGSQEFSFSPGHVKPHEIALDIGTAGSIPLVIQAW
                     LPVALKSGGSATLRGGTEVEKSPTIDYMAHVLVPFLEMHGARISLDIARRGYFPKGGG
                     KVRVMVEPSVLQPLSPECLRPEGGRGGCSCSAGLPPHVAERQARAAARLLMRRTGLDF
                     SIPTDTRQGPGTGSSCTVWMGWMGGTGLGRRGYPAEKVGEDAAHALIRELEGGGLVDS
                     FMADQLLVYLGMAGGAYTSHACTPHARTMCWLLGQFGIHIRVTMEEVTRFERAACA"
     gene            1883..2338
                     /locus_tag="PKM87_07390"
     CDS             1883..2338
                     /locus_tag="PKM87_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNQ29928.1"
                     /translation="MRRVLDATAFFVEMPLEGELFTSPLVVDELADARSRCRFEAFSA
                     AGLQVIAPGRDSIQRVEEVSQKSGDAGVLSAADVEVLALAIEISAEVVSDDFAIQNVA
                     HHLGIPVRPLQQRRAKKRTWRFLCPGCGRYSDRPGECPVCGAVPKRTIK"
     gene            2360..2980
                     /locus_tag="PKM87_07395"
     CDS             2360..2980
                     /locus_tag="PKM87_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase-like protein"
                     /protein_id="HNQ29929.1"
                     /translation="MSSLDELIQKARMLLSEGHSPGQIADELSLSMETVTWLLTQQKG
                     AIIPKDVHIDWTAVSGQGPLLDGVAMMLLKRYYSALDEGEKEEGYSERFPTLAVGIAV
                     SGIPLATLIAVEEDIKLAIYHPAKHSTQDPPVGSISGNFSSVTGERCIIIDDVITSGN
                     TMQDVVKYLRRHGATPVAIWVIFDKRGLRDVDGVPVYSLFKISRID"
     gene            3064..4677
                     /gene="thsA"
                     /locus_tag="PKM87_07400"
     CDS             3064..4677
                     /gene="thsA"
                     /locus_tag="PKM87_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HNQ29930.1"
                     /translation="MLGGQPIIILKENVERNRGLEAQRSNIAAAKAIAGAVRTTLGPR
                     GMDKMLVEGSGDVTITNDGATILQDISVQHPGAKMVIEVARAQDEEVGDGTTTAVVLV
                     GSFMEKAEQLIEKKIHPTVIAEGYRMGMKKALEVLEDLSFTIDPYDREKLIKIADTAM
                     TGKSIESVKNKLNGIAVDAVMAIAEKEGKKITADEDNVLIKKQAGDTMDDAELVRGVV
                     IDKKRISEDMPRKVRDAKIALVASPMEIKKTQVKSKIHISSSEMVSAFSAQEREALKK
                     FADKVIESGANVLLCQKGLADAVAFYLAKGGVLAVEDVPEKDMKFAARALNAQIANKV
                     DELSLDMLGRAELVEQLDDGEFVKISGCENPKTVTILLRGSTEYLLDELERAVVDGTR
                     VVMDAMEDGKLVVGGGAVETEILMKVRDYADNVGGRVQLAIEAYANAFEVIPVTLAEN
                     SGYNPVDKLVDLKNAHARKQKHAGLNVYNGKIIDMLKEGVVEPQRSKRQAIQSATEAA
                     IMLIRVDDMMVTREGGAPGGMPAGMPPMG"
     gene            complement(4762..4971)
                     /locus_tag="PKM87_07405"
     CDS             complement(4762..4971)
                     /locus_tag="PKM87_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine S protein"
                     /protein_id="HNQ29931.1"
                     /translation="MEVKCRLFLVPGGECVEYEGAEGATYEEALLSIGIIPDTVLILF
                     RGESLPQDKSIEEDEVEIVSTCSRG"
     gene            5015..5099
                     /locus_tag="PKM87_07410"
     tRNA            5015..5099
                     /locus_tag="PKM87_07410"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5049..5051,aa:Ser,seq:cga)
     gene            complement(5247..5780)
                     /locus_tag="PKM87_07415"
     CDS             complement(5247..5780)
                     /locus_tag="PKM87_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29932.1"
                     /translation="MPTFFAPVQNNPPRIAMNDDPGTNAISPLCMVCAHLHRKARPPA
                     CDAFPGGIPEDILAGRPHVTPYPGDRGIEFRKTSGEPSFFIVGSHPGTLFRVTCAGSY
                     ERYVPILDLWELDNMFICYVLSVPLPIQAVDPVEADAFVDSARKCLDAMNPCMREVRR
                     MMYLSAHGGITQGGDRG"
     gene            complement(5811..6782)
                     /locus_tag="PKM87_07420"
     CDS             complement(5811..6782)
                     /locus_tag="PKM87_07420"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNQ29933.1"
                     /translation="MRTGIPLAIFILGFLVIATGCMQTSPPAGPSTPLSTHTTPEPIV
                     SPNATVSPGHHYPATSLEIKTYVDNAVSFAQENGKEAAIAAFNNASGPFVNGDVYVYA
                     LDYSGIALALPFQPGMVGTDFRPLKDATGKPYTDIEIQLAKGGGGYILYRYPYPADEQ
                     QSTLKISYVRPVDDTYWIGAGVYTREDLLVDDELRQFVAEAKAYAVANGREKAVAAFN
                     NQTGPFIRGELYIFAYDYNGTVLSWPYQPEQVGVNRLDVTDPVGNHHVRDFLDTAKGG
                     GGMVDYYSVNPFTNRTDLKISYVTDVDGTWMLGAGKYIEPGQVILRA"
     gene            complement(7118..9382)
                     /locus_tag="PKM87_07425"
     CDS             complement(7118..9382)
                     /locus_tag="PKM87_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ29934.1"
                     /translation="MRDTRWRLPFSYLLMGILFLVIVAVLIAGLWINYENSKNHTVEN
                     AERLRVMTESHMDNSFRMIDTGLKLYDNTYNQKMQDAFGIVMAEYLQAGGEPSLMDLP
                     SLKNRIGGMEIHVINDQCVIEHSSSPGDIGLDFSIIYPDFCVYLHKIQNTSGYYPDRV
                     VMEWFTGNLTKYGYMPTPDHQYILELGLHSDAFTRDRMELQYRDVIDEVRTFNPYLEE
                     VLLFQKQKRLIYNTTYIPTLEESEMLDYILWKNRSTQIVKDNGAGKTIVWQVIDLRDP
                     DYAADKSVFAKLTYNDALVREELDQIAILHGFAALLVILSGGLIAIMVSRKLSRPIER
                     LVEDVNIIAEGDLNHAVRPAPGFELSNLSGSIQVMVDRLKAQIRQCEKSEERFTELVQ
                     LLPQGVFETDLNGNITFANPIAFEVFGYNPEDFENGINIFDILAPDEQILARERFREI
                     LAGKRSQGSEYTGIRKDGCTFPIMVYTAARKEEGAVTGMRGTIVDISHLKKVEEEIRK
                     LNAELEDRVAQRTSELEYATREMEAFTYSVSHDLRGPLRAIDGFSHIISESASTGLDE
                     RELHCIDVIRQNVVLMDSLIDGLLALSRMGRQELKRERISPEPLVREVVAEVLNQAPD
                     RKIDVTIGTLPECNADPVMLRQVFANLIGNAVKFTRKTDNARIEIGAFFRDSQAVYYV
                     RDNGAGFDMKYVNKLFKEFQRLHRPEEYEGSGIGLAIVDRIIRRHGGKIWAESEPGKG
                     ATFYFFLEGHPEHS"
     gene            9674..10165
                     /locus_tag="PKM87_07430"
     CDS             9674..10165
                     /locus_tag="PKM87_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29935.1"
                     /translation="MRAIFFVVLCALAGLLVLGAGCTQPSTGPATQVQTPVPTTAQPT
                     ASPTVATPVATPTLGYIEGPLPAQYMVDVQVDRNTVAITPSIIATYRGGKGTNFVYSV
                     DVTVTRSDGQVITKSLERPQVNQQVEIEGTRGTDRVEVFVNLVNGERYRIYDQSLAFR
                     TYN"
     gene            complement(10365..10793)
                     /locus_tag="PKM87_07435"
     CDS             complement(10365..10793)
                     /locus_tag="PKM87_07435"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNQ29936.1"
                     /translation="MSPPHPSDSDHLSPADVHEKVRRFNHSEFARLLEMEILEARPGY
                     ARVRMDTEGKRNLRGFAHGGAIFALADQAFGIAANLGRTHEVALSAQISYLVPAKGTL
                     IAVAECTGQTPQYSSYVVRVFHDDVLVATFDGQGMKQHDK"
     gene            complement(10840..11544)
                     /locus_tag="PKM87_07440"
     CDS             complement(10840..11544)
                     /locus_tag="PKM87_07440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ29937.1"
                     /translation="MRISVVVPSFNEAANITACLGSLNRQTVPRDEYEIIVVDGGSKD
                     TTRELAEPLADTVFIQTSKKVGGARNDGALRARGSILATTDADCIIPSNWLEVILDDF
                     ERHHDVVQIYGPVDPIEKTIRNHLSLAMANNFARFGYYTHTLYYTLGCNTAFRKDAFL
                     RAGMYHVIDAGDDLEIARRMRQEGRVMFDSRMRVGFSMRRYEQFGTLKSLYEWLYIVA
                     HGGSSEKHQYTRREYK"
     gene            complement(11541..12515)
                     /locus_tag="PKM87_07445"
     CDS             complement(11541..12515)
                     /locus_tag="PKM87_07445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNQ29938.1"
                     /translation="MKVNFFVEDMLFFRFIGCATVAKTLYRQLQNEPGIEVSWKSYSN
                     HADVVHYHTFGPLAGLNRRMAKGTKVLTAHSTPRINMENIALARAINKRYPKIYRRFD
                     HIITISRPCHEEVTAMIPDMPVTMIPNGVDREFFRRDEEKRAAFREYYGIPDDQQVVL
                     TVAQQTPRKGLYDFLALSRMLPDKKFVWIGGFPYGALSKNYGKIKQMKLRCGGNVLFP
                     GYINDILKPYSAADLFFMPSYAETFGLVNLEALACGLPVVARDIPEFHEIYGNNILYF
                     SERADAVARIEDEAALHQCARGARDFTSQFDLRDIAAQHIRLYRELSE"
     gene            complement(12512..13825)
                     /locus_tag="PKM87_07450"
     CDS             complement(12512..13825)
                     /locus_tag="PKM87_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ29939.1"
                     /translation="MRTLVLGGGLTGITLAHLLRQRGQEATVLEAAERIGGLCRSRSE
                     NGFTFDVGGSHIIFSRDHEVLEFMKNVLGSNREQRTRNTRILFRDILVKYPFENGLAA
                     LPKDDLFYCINEFIQTYLKAEKGEIPPPANFREWIYATFGKGIADLYMVPYNEKIWNY
                     PPESMSAHWVEGRVPRPPVEDVIKSAIGIETEGYTHQSVFTYPAEGGIESLVKSIARG
                     IEDCILTGFTVRSLAFRGGVWEAGDGKRTVTGDRVISTIPLQHLLPCLGGGVPPAVKE
                     AVGALRYNSVCCVQIGIKGRVPDISWLYIPYRTQSLANRISFPSNFSSHVAPPGHAGI
                     LAEITYNEGDEVSRMTDDELVSHVIGSLERMGFLTPGQVVHTAVDRQKFAYVVYDLDY
                     PGNIRIIREYFQERGITLVGRFSQFEYLNMDGCIRQAMECARSWP"
     gene            13940..14392
                     /locus_tag="PKM87_07455"
     CDS             13940..14392
                     /locus_tag="PKM87_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HNQ29940.1"
                     /translation="MSLQEYSKKRDFSKTPEPRGAGGEGGGVPLFVVQEHHSSHHHFD
                     FRLEHEGVLASWAVPKGVPESPGEKRLAVRTEDHPLAYADFEGSIPEGEYGAGEVKIW
                     DRGTFVLNTWEADRIEVILQGNRLNGRYLLVRFKRAGENDWLLFRAKE"
     gene            complement(14439..>14645)
                     /gene="ilvN"
                     /locus_tag="PKM87_07460"
     CDS             complement(14439..>14645)
                     /gene="ilvN"
                     /locus_tag="PKM87_07460"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNQ29941.1"
                     /translation="RAEIMQIASIFRAQIIDVGSRTVVLSVAGDTEKIDALEKLLRQY
                     GVKELVRTGKIAILRGAKTVKSSK"
BASE COUNT         3123 a         4090 c         4204 g         3228 t
ORIGIN      
        1 agatagtgat ataatttatt cctgctcgtt taagtacttt cagcctgagg tagcggaaat
       61 gttggtcgta agcacagaat attcccagat cctggccgca aacgtggcaa atgccctgga
      121 atgtcccctg ggcggggtaa ggttcacacg gtttcccgat ggcgagcatt acctccgcat
      181 gacggcgacg gagagggaga tggtggtcgt gggaagtgtc cccgattcag atgccctggt
      241 ccagctcctc ctccttgtcg atgcctgcga tggcgccgag gtcaccctcg tcgtgccgta
      301 catgggatac gccaggcagg acaagcagtt ccatgccgga gaacctctca gcgcccgggc
      361 gatcgcaagg gtgctctcga caggggtatc acgggtcttc acggtcaacg tccacgacag
      421 ggctgtcctc tcccatttct gcgtgcctgc acatgatctg agcctggcag cggacatcgg
      481 gcgctatctc tctacaaaat ccctcacccg tccccttgtc cttgctccgg acgacggtgc
      541 ggccgcgttt gcacacaggg ttgcgtcagt gtgcgggtgg gaatccgatc acctggaaaa
      601 gaccaggctc tcgggggcag aggtgaagat ggagccacgg cgcctcgagg cgaatggccg
      661 cgacgtggtg atcgtggacg acatcatctc gaccggagga accctggcga ccgcagctgc
      721 catgctgatg gagcagggag cacaaagcgt cacatcggtg tgtgtccatg gtgtcctcgc
      781 cgaaggcgca tatgccaggt tgctctccgg cagtatccgg gaagtggcct gcagcgatac
      841 cctcgagagg gcatgcagcc gttactccgc ggggcgtgcc attgccgacg ggataaggcg
      901 atgctgagca tcgacggcgc gagcctggag gggggagggc agattgtcag ggcagcggtt
      961 gccctctcgg ccctcaccgg cacccctata aggatcgaac gtgtccgggc gggaagagaa
     1021 cagccaggtc tccggcccca gcacgtcgca gcagtgcgtg cggttggggc agcctgcagt
     1081 gcggaggtca tcgggtgcct ccccggcagc caggagtttt cgttttcccc tggccatgtg
     1141 aaaccccacg agatcgctct tgacataggt actgcgggga gcattcctct agtcatccag
     1201 gcctggctcc ccgttgcatt gaaatcaggg ggttcagcga cacttagggg aggaacagag
     1261 gtcgaaaaaa gccctaccat cgattacatg gcccatgtac tcgtcccttt cctggagatg
     1321 cacggagccc gcatctctct tgatatcgcg aggcgcgggt atttcccaaa aggcgggggc
     1381 aaggtaaggg tcatggtgga accctccgtt ctgcagccac tctctcccga atgtctccgg
     1441 ccggagggag gccgcggggg atgctcctgc agtgcggggc tcccgcccca cgtggcggag
     1501 cggcaggcgc gtgcagcagc caggcttctc atgcgtcgca cagggcttga cttctccatt
     1561 cccaccgata caaggcaggg cccaggcacc ggaagctcgt gtaccgtgtg gatggggtgg
     1621 atggggggaa caggactagg gaggaggggg tatcctgccg agaaggtggg ggaggatgca
     1681 gcccacgcac ttatccgcga gcttgaggga ggcggcctcg tcgattcatt catggctgac
     1741 cagctcctcg tctacctcgg catggcgggt ggcgcctata ccagccatgc ctgcacgccg
     1801 catgcacgca ccatgtgctg gctgcttggg cagttcggaa tccatatcag ggtcaccatg
     1861 gaggaggtga cgaggtttga gcgtgcggcg tgtgcttgat gccaccgctt ttttcgttga
     1921 gatgcccctg gagggggaac tctttacctc tcccctcgtg gtggatgaac tcgccgatgc
     1981 aaggtcacgg tgcaggttcg aggcgttctc cgcagccggg ttgcaggtga tcgctcccgg
     2041 cagggactcc atacagaggg tggaagaggt ctcgcagaag agcggggatg caggggtgct
     2101 ctccgcagcc gatgtagagg tcctggccct cgcaatcgag atatcggcgg aagtggtgtc
     2161 agacgatttt gccatacaga acgttgcaca tcacctcggg atccccgtcc gcccactcca
     2221 gcagcggagg gcgaagaaga ggacgtggag gttcctgtgt ccgggatgcg ggaggtactc
     2281 tgacagacct ggagagtgcc cggtctgcgg ggcagtcccg aaaagaacca ttaagtagga
     2341 gacagaatac tgtttcacca tgtcgtcgct cgacgagctg atccagaaag caaggatgct
     2401 cctctccgaa ggccacagcc ccgggcagat cgccgatgaa ctctcgctct ccatggagac
     2461 cgtgacctgg ctgctcaccc agcagaaggg ggcgatcata ccaaaggacg tgcatataga
     2521 ctggaccgcg gtcagcggcc agggccccct ccttgacggg gttgccatga tgctcctgaa
     2581 acgctattat tcggccctcg acgagggtga gaaagaggag ggctattccg agcggttccc
     2641 cacactcgcg gtggggatcg ctgtctccgg aatcccactg gccaccctga tcgcagtcga
     2701 ggaggacatc aagctcgcca tctaccaccc tgcaaaacac agcacccagg accccccggt
     2761 gggttctatc agcgggaatt tttcatctgt tacaggggag aggtgcatca tcatcgacga
     2821 tgtcatcact tcggggaaca cgatgcagga cgtggtaaag tacctgcgcc gccacggggc
     2881 tacccctgtt gccatatggg tgatatttga caagcgcgga ctccgggacg tggatggcgt
     2941 tccagtctac tcgctcttta aaatatcccg catcgattga tatccctccc atttttccgt
     3001 cgaaaaatag attagtttat atagaagttt aattccatct tttacaacat tggaggaatt
     3061 gcgatgcttg gaggacaacc tatcatcatt ctcaaagaga acgtagagcg gaacaggggc
     3121 ctggaggccc agcgctcgaa tatcgcggct gcaaaggcca ttgccggcgc ggtcaggacc
     3181 acgcttggac cccgcggtat ggacaagatg ctcgtggagg gttccggcga cgtgaccatc
     3241 accaacgacg gggcgaccat cctccaggat atctccgtcc aacaccctgg cgcaaagatg
     3301 gtcatcgagg tggccagggc acaggacgag gaggtagggg acggcacgac caccgcggtg
     3361 gtcctcgtag gctcgttcat ggagaaggcc gagcaactga tcgagaagaa gatccacccc
     3421 accgtgattg ccgaaggata ccgtatgggc atgaagaagg cgcttgaggt cctcgaagat
     3481 ctctccttca ccatcgatcc atacgacagg gagaagttga tcaagatcgc agacaccgcc
     3541 atgaccggga agtcgatcga gtcggtcaag aacaagctga acgggattgc ggtcgatgcg
     3601 gtgatggcca ttgccgagaa agagggcaag aagatcaccg cagacgagga caacgtcctg
     3661 atcaagaagc aggcaggcga caccatggac gatgccgagc ttgtcagggg cgtggtcatc
     3721 gacaagaagc gtatcagcga ggacatgccc cggaaggtca gggacgcaaa aatcgccctt
     3781 gttgccagcc ccatggagat caagaagacc caggtcaagt ccaagatcca catcagctca
     3841 agcgagatgg tcagcgcgtt ctctgcccag gagagggaag ccctgaaaaa gttcgccgac
     3901 aaggtcatcg agagcggggc aaacgtgctg ctctgccaga aaggccttgc cgatgccgtc
     3961 gccttctacc tggccaaggg cggtgtcctt gcagtcgagg acgtccccga gaaggacatg
     4021 aaatttgccg caagggcgct caatgcccag attgccaaca aggtcgatga actctccctc
     4081 gacatgcttg gccgggccga gctcgtggag cagctggatg acggggagtt cgtcaagata
     4141 tcaggctgcg agaatccgaa gaccgtcacc atactcctcc ggggctcgac cgagtatctc
     4201 ctcgacgagc tcgagcgggc ggtggtggac gggacccgtg tcgtgatgga cgccatggag
     4261 gacgggaaac tggtggtcgg cggcggggcc gtagagaccg agatcctgat gaaggtcagg
     4321 gactatgccg acaacgtcgg gggccgcgtg cagctcgcca ttgaggccta tgcaaacgcg
     4381 ttcgaggtca tccccgtcac ccttgccgag aactcaggct acaacccggt ggacaagctg
     4441 gtggacctca agaacgccca tgccaggaag cagaagcatg cgggcctgaa tgtgtacaac
     4501 gggaagatta tcgacatgct gaaagagggc gtagtcgaac cccagcgctc caagcgccag
     4561 gccatccaga gcgcaaccga ggccgccatt atgctcatca gggtggacga catgatggtc
     4621 acccgcgagg gtggtgcccc cggaggcatg cccgcaggca tgcccccgat gggatgaaac
     4681 tctcacacac cttttttttc ttattttaca cccaggtgct tccctgatct ggttttttta
     4741 aatcatatcc agctcttcag gttatcccct ggagcaggtg gagacaatct ccacctcgtc
     4801 ttcttcgata gatttgtcct gggggagact ctcccccctg aagaggatga gcacggtatc
     4861 gggtatgatc ccgattgaga ggagtgcctc ctcgtacgtt gcgccttccg ctccctcgta
     4921 ttcgacacat tccccaccgg ggaccaggaa aaggcggcac ttcacttcca ttcgatagga
     4981 ttatacgaat acgaaggcaa aatatctacc ccgtgccgag gtagtctagt ccggaaaggc
     5041 ggtagcctcg aaagctactg gcgctctgcg cctcgggagt tcaaatctcc ccctcggcgt
     5101 gttatcgcat ccttttcatt atttttttta tgctccgttc tacatctatt ctgaatcaca
     5161 tagtatctca tctgacagcg ggtgttcctg ggaatatgcc cggttgcccc gaacgctcgt
     5221 cgacccctaa tctcacagct gtcagcctat cccctgtcgc ccccctgtgt aatcccgcca
     5281 tgggcgctca ggtacatcat ccgcctgacc tcacgcatgc aggggttcat ggcatcgagg
     5341 catttccggg cggaatcgac aaaggcatcg gcctcgaccg gatcgactgc ctggatcggg
     5401 aggggaacgg agagcacgta acatatgaac atgttatcca gctcccacag gtcaaggatt
     5461 gggacgtagc gctcgtagga cccggcacag gtcacacgga agagtgtccc gggatgcgac
     5521 cctacaatga agaatgatgg ttcgcctgaa gttttccgga actcaatccc gcgatcaccc
     5581 gggtaaggag tgacatgcgg ccttcccgcg aggatatcct ccggaattcc tccgggaaat
     5641 gcgtcgcatg caggaggccg ggccttgcgg tggaggtggg cgcagaccat gcagagaggg
     5701 gagatcgcgt tcgtgcccgg atcgtcattc atggctatac gaggagggtt attctggaca
     5761 ggggcgaaaa aggttggcat ggaggatcgc tcctgccatc gtgcattcct tcaggcccgc
     5821 aggatgacct gcccgggctc tatgtacttg cccgcaccga gcatccaggt cccgtcgaca
     5881 tcagtcacat agctgatttt caaatccgtc ctgttggtaa atgggttcac agagtagtaa
     5941 tcgaccattc ccccgccgcc cttcgctgtg tcgaggaaat ccctgacgtg atggttgccg
     6001 acagggtcgg tcacatcgag ccggtttacg ccgacctgct ccggctggta gggccagctc
     6061 agcactgtcc cgttgtaatc gtaggcgaat atgtagagtt ctccgcgtat gaatggtcca
     6121 gtctggttgt tgaacgcggc cacggccttt tcacggccgt tagccactgc gtacgccttt
     6181 gcctctgcga caaactggcg aagctcgtcg tcgacaagga gatcctcacg ggtgtatacc
     6241 ccggcgccaa tccagtaagt gtcgtcgacg gggcgcacat agctgatctt cagcgtgctc
     6301 tgctgttcat cggcgggata cgggtagcgg tagaggatat acccgccgcc tccttttgcc
     6361 agttggatct caatatcggt atacggtttt ccggttgcat ccttcagcgg gcgaaaatcg
     6421 gtgccgacca tgccgggctg gaaggggagg gcaagtgcga tgcccgaata atcaagggca
     6481 tacacataga catcgccatt gacgaagggg ccggatgcat tgttgaatgc ggcaatggct
     6541 gcctcctttc cgttctcctg cgcgaaggat acggcgttgt cgacgtaggt tttgatctcg
     6601 agcgacgttg cgggataatg gtgaccaggg gatactgtgg cattgggaga aacaatgggt
     6661 tctggcgtcg tatgggttga caagggggtc gaaggaccgg cgggagggga tgtctgcatg
     6721 cacccggtcg caatgaccag gaatccaagg atgaagatgg cgagaggaat tcctgttctc
     6781 atgttcgatc agtacttcgc ggctcctctt aaaatgaaca gatttattat gtgagcgaag
     6841 gggtatccca tcccgttttt cggcctggtt tgccattctt ccccggcaac tgtcggtcag
     6901 cactttcgcg agttaagaat cgcgcggggt gtcttcaaaa gatacccccc ctgatccatg
     6961 tgtggtgccc tcaaaggaca tccttcatgt gaatcttatc acttttttaa aaattcaaat
     7021 tacaggctgg cccctgtaga caatggaacc ctctccaacc acccggaggt cccgggatgc
     7081 aaggtggaat gaggggaaaa gtcccggtaa tcctttttca ggagtgttca ggatggcctt
     7141 caaggaagaa atagaaggtc gcccccttcc cgggttcgct ctcagcccag atcttcccgc
     7201 catgccgccg gatgatgcgg tccacaattg caaggccgat acctgaccct tcgtactcct
     7261 ccggcctgtg cagccgttgg aattccttga aaagtttatt tacgtacttc atgtcaaagc
     7321 cggcaccgtt gtcccggaca tagtacactg cctgggaatc cctgaagaaa gctcctatct
     7381 cgatccgggc attgtccgtt ttcctcgtaa attttaccgc attgccgatg aggtttgcga
     7441 acacctggcg cagcatcacg ggatcggcgt tgcactccgg caatgtccca atcgtgacat
     7501 cgatcttccg gtccggggcc tggttcagta cttcggcaac aacttcccgc accagcggtt
     7561 caggtgatat ccgttcgcgc ttcagttcct gccggcccat gcgcgagagt gccaaaagac
     7621 cgtcgatgag actgtccatc agcaccacgt tctgccttat cacgtcgatg cagtgcagtt
     7681 ccctttcatc gagacctgta ctggctgact ctgagattat atgggagaac ccgtcaatag
     7741 cccgaagcgg gccgcgcagg tcatgcgaga ccgaataggt gaacgcctcc atttcgcggg
     7801 tcgcatattc gagttccgat gtccgctgcg caacccggtc ttccagttcg gcattcagtt
     7861 ttcttatctc ttcctcgacc ttctttaagt ggctgatatc tacaatcgtt ccccgcatac
     7921 cggtgacagc cccctcctct ttccgtgccg cggtatatac catgatgggg aatgtgcagc
     7981 catctttccg tatccctgta tattcagaac cctgggacct cttccctgcc aggatctccc
     8041 tgaatctctc tctcgccagt atctgttcgt cgggtgcgag gatgtcgaat atgttgattc
     8101 cattctcgaa atcttccggg ttatacccaa agacctcgaa cgcgatgggg ttggcaaaag
     8161 tgatgttccc gttgagatcg gtctcaaaaa ccccctgggg gaggagctgg acgagttccg
     8221 tgaagcgttc ctcgctcttc tcgcactggc ggatctgtgc cttcagccgg tcgaccatga
     8281 cctggatgct cccggaaagg ttggaaagtt cgaagcctgg agcgggcctg accgcatgat
     8341 tcagatcccc ttctgcaatt atgttgacat cctcgactag tctctcgata ggcctcgaaa
     8401 gttttcgcga gaccatgatg gcgataagcc ctcctgaaag aatgacgagc aatgcggcaa
     8461 agccatgcag gattgcaatc tggtccagtt cttctctcac cagtgcatca ttataggtga
     8521 gttttgcaaa tacactcttg tcagcggcat aatccgggtc acggaggtcg atgacctgcc
     8581 agacgatggt ctttcccgca ccattgtcct tcactatctg ggtagaccgg ttcttccaga
     8641 ggatgtaatc gagcatctcc gattcttcaa gggtgggaat ataggtggta ttgtagatca
     8701 gcctcttctg tttctggaac aggaggactt cttccaggta aggattgaat gtcctgacct
     8761 cgtctatcac atcgcgatat tggagttcca tccgatccct cgtaaatgcg tctgaatgaa
     8821 gcccaagctc gagaatatac tggtgatcgg gtgtgggcat atatccgtat tttgtgaggt
     8881 tcccggtgaa ccattccatc acgaccctgt cagggtaata gcccgaagtg ttctgaatct
     8941 tgtggagata tacacagaaa tcggggtaga taattgaaaa atcgagccct atatcccccg
     9001 gtgacgatga atgctcgatg acacactgat cgttgatcac gtggatttcc atccccccga
     9061 tcctgttttt cagggaggga aggtccatca gggagggctc tcctcctgcc tggagatatt
     9121 ctgccatgac gattccgaac gcatcctgca tcttctggtt gtaggtgttg tcgtacagct
     9181 tcaacccggt gtcaatcatc ctgaacgaat tatccatgtg cgattccgtc atgacccgga
     9241 gccgttctgc attctcgacg gtatggttct tcgaattctc ataatttatc cataacccgg
     9301 cgattaatac agcgacgatg acaagaaaaa ggattcccat gaggaggtat gaaaaaggca
     9361 gtctccaccg ggtatctcgc atcaaatccc cctttcaatg ggatatatgc aattatgtta
     9421 tttaacagga atcgtcagcc agccgggtca tgtttaggat ggatattcat cccaaagact
     9481 tcagtattcg aacgtaaccc ctctcatcat atgcgttgga aggtgcaatc cggtgaatcc
     9541 ggacccggaa ggtcctgtgt gtagccagca taaagttccc ctcttttgat ttcggcagtc
     9601 ctgtaaggtg accttgcaaa atgaaaaatg gatcctcaga agactcttat aattgcggga
     9661 tgaagctcta ctcatgagag cgatcttctt tgtggtgctc tgtgcgcttg caggactcct
     9721 tgtccttggc gccggctgca cccagccatc aacggggccg gccacgcagg tccagacacc
     9781 tgtgcccacc acggcacagc ccaccgcaag cccgactgtt gcgacccctg tcgcgacccc
     9841 gacactcgga tacatcgagg gtccgctgcc tgcacagtat atggtcgatg tccaggtcga
     9901 ccgtaatact gttgccatca ctcccagcat catcgcgaca taccggggtg gaaaaggcac
     9961 caattttgta tattctgtag atgtcacggt taccaggtcc gatggccagg tgatcacgaa
    10021 gagcctggag aggccgcagg tgaaccagca ggtggagatc gaagggacac ggggcaccga
    10081 ccgggtagag gtgttcgtca accttgtcaa tggggagcga tacaggattt acgaccagtc
    10141 cctggcattc cggacataca attaatatcc tctttttgat tattttgcgg cggtaatgaa
    10201 tctcggttaa gtaaatttca tgaatttgcg ggattcctta aagacgcttc caggatattc
    10261 attctgatgt attccgggtt gcagggcagc cagttctcac ctggtatact ttccaagggg
    10321 atctggaaaa gaccaggcgt gggatttata ccgggaatcg gccttcactt gtcgtgctgt
    10381 ttcatcccct ggccatcgaa tgttgcaacc agcacatcgt cgtgaaagac tcgcaccacg
    10441 taactggagt actgcggggt ctgacccgtg cactcggcaa ccgcgatcag ggtacctttt
    10501 gcagggacaa gatagctgat ttgggcagac agggctacct cgtgggtgcg accgaggttt
    10561 gccgcgatgc cgaatgcctg gtcggcaagc gcgaagattg caccaccatg ggcaaacccg
    10621 cgcaggttcc gcttcccttc cgtgtccatc ctcacccgtg catacccggg acgcgcctcg
    10681 agtatctcca tctccagcag gcgggcgaac tccgaatggt tgaaacgccg gaccttctca
    10741 tgcacatcgg caggagagag gtgatcggaa tcagatggat gcggagggga cattgagtat
    10801 cgcattctca tttgggaggc gaaaaacctc aaaacaccct catttgtatt ccctgcgcgt
    10861 atactggtgc ttctcggacg aaccaccatg tgccacgata tagagccatt catagagtga
    10921 cttgagcgtc ccgaactgtt cgtacctgcg cattgagaat ccaaccctca tcctgctgtc
    10981 gaacatcact cttccttcct gcctcatcct tcgggcaatc tccaggtcat cgccggcgtc
    11041 gatcacgtga tacattccgg cccgcaggaa cgcgtctttc ctgaaggccg tgttacaccc
    11101 gagagtatag tagagagtat gggtgtaata gccaaaccgt gcgaaattgt tcgccatggc
    11161 aagagagagg tggttcctga tggtcttctc gatggggtcg actggcccat agatctgcac
    11221 gacatcgtgg tgcctttcga agtcatcgag tatcacctcg agccagttgg atgggatgat
    11281 gcaatcggca tcggtggtcg cgagaattga acctctggcc cttagtgcgc cgtcgtttcg
    11341 tgcccccccc accttcttgc tggtctggat aaagaccgtg tcagccaggg gttcggcgag
    11401 ttccctcgtg gtatccttcg atcccccgtc cacgacaata atctcgtact cgtccctcgg
    11461 gacagtctgg cggttcagcg acccaaggca tgcggtaatg ttcgcagcct cgttgaaaga
    11521 cggcacaacg acagagatcc tcattctgac agctcccgat acagcctgat atgctgcgcg
    11581 gcgatatccc tgagatcgaa ctgggaggtg aaatcccttg cccccctggc acactggtgc
    11641 agtgctgcct cgtcctcgat ccgcgccacg gcatcagccc gttccgagaa gtagaggata
    11701 ttgtttccat aaatttcatg aaactcaggg atgtctcgtg caaccacggg gaggccgcat
    11761 gcaagggctt caaggttaac caggccgaac gtctcggcgt aggacggcat gaagaagagg
    11821 tctgcggcgc tgtatggctt caggatgtcg tttatgtacc ccgggaagag cacgtttcca
    11881 ccgcaccgaa gtttcatctg cttgatcttc ccgtagttct tcgagagcgc cccgtaggga
    11941 aaccccccga tccagacgaa cttcttgtca gggagcatcc gggagagtgc caaaaagtcg
    12001 tagaggcctt tccttggtgt ctgctgtgcc acggtgagca ctacctgctg gtcatcgggg
    12061 atcccatagt attcccgaaa cgcggccctc ttctcctcgt ccctcctgaa aaactcccgg
    12121 tccacaccgt ttgggatcat cgtcaccggc atgtcgggta tcattgcggt gacctcctcg
    12181 tggcagggcc tggagatggt gatgatatgg tcgaacctcc ggtagatctt cgggtatctc
    12241 ttgttgatcg cccgtgcaag ggcgatgttt tccatgttga tccttggtgt ggagtgcgcg
    12301 gtgagaacct tcgttccctt cgccattcgg cggttgagac cggcgagagg gccgaacgtg
    12361 tgataatgca cgacgtccgc gtggttcgag taactcttcc aggagacctc gatccccggc
    12421 tcattctgga gctggcggta gagtgtcttt gcaaccgtcg cgcaccctat gaatcggaag
    12481 aagagcatgt cttccacgaa gaaattgacc ttcacggcca gctccttgcg cactccatgg
    12541 cctggcggat gcacccgtcc atgttcaggt actcgaactg cgagaacctc cccacgagcg
    12601 tgatcccccg ctcctggaag tattccctga tgatcctgat atttccgggg taatccaggt
    12661 catacaccac gtacgcaaac ttctgccggt ccaccgcagt gtgcaccacc tgtcctggtg
    12721 tcaggaaccc catcctctcc agggacccaa tcacgtggct gacaagttcg tcgtcggtca
    12781 tccgggagac ctcgtcgccc tcgttgtagg tgatctcggc gaggatacct gcgtgccctg
    12841 ggggtgccac atggctgctg aaattggagg gaaacgagat gcggtttgcg aggctctgtg
    12901 tcctgtaggg gatataaagc catgaaatgt cgggaaccct cccctttatc ccgatctgga
    12961 cgcagcagac ggagttgtag cggagggcac cgactgcctc cttgacggcg ggagggactc
    13021 ctcccccgag gcatggcagg aggtgctgca gcgggatggt gctgatcacc cggtctcccg
    13081 tgactgtccg tttcccgtct ccggcctccc agactccgcc acggaacgca agcgaccgga
    13141 cagtgaatcc ggtcaggata cagtcctcga ttcctcttgc aatggatttg accagtgact
    13201 caattccacc ctctgccggg taggtgaaga ccgactgatg ggtataccct tcagtctcga
    13261 tccctatggc agattttatc acgtcttcca cgggaggacg cggaaccctc ccctcgaccc
    13321 agtgggcaga catggactcg ggggggtagt tccagatttt ctcattgtaa gggaccatgt
    13381 agagatctgc aatccccttt ccaaatgtcg cgtaaatcca ttccctgaag ttggcaggag
    13441 gggggatctc ccccttctcg gctttcaggt atgtctgtat gaactcgttg atgcagtaga
    13501 agagatcgtc cttgggaagg gccgcaagac cattctcgaa tggatatttc acgagaatat
    13561 cccggaacag aatcctggta tttctcgtcc gctgctccct gttggagccg aggacgtttt
    13621 tcatgaactc gagcacctcg tggtcgcggg agaagatgat atgcgatccc ccgacgtcga
    13681 aagtgaaccc gttctctgaa cgggagcggc agagccctcc tatcctttct gcagcttcga
    13741 gtactgtcgc ctcctggcca cgctgccgca gcaggtgtgc gagagtgatt ccggtaagcc
    13801 ctcctcccag gacgagtgta cgcacgagca tactattaaa cgggagggat tatatgattg
    13861 accatccacg agtgtgttct gatgcgcaag ctgttgacgg agagaaaaag aacgacgtta
    13921 tgaacccggg gttgaactca tgagcctcca ggaatattca aagaaaaggg atttttcgaa
    13981 gacacctgaa ccgcgcgggg ccggcggaga aggtggcggg gtcccgttgt ttgtcgtgca
    14041 ggaacaccat tcaagccacc accatttcga cttccgcctg gagcacgaag gggtgcttgc
    14101 atcctgggcg gtgccaaagg gcgtcccgga gtcgccgggg gagaagcggc tcgcagtccg
    14161 gaccgaggat cacccgctcg cgtatgctga cttcgaggga agcatccccg aaggagagta
    14221 cggcgcagga gaggtgaaga tctgggaccg cggcaccttt gttctcaaca cctgggaggc
    14281 cgaccggatc gaggtgatat tgcagggaaa ccggctcaat gggcggtatc tcctcgtccg
    14341 gttcaaacgt gcgggggaga acgactggtt gttattcagg gcaaaggagt gaatggctga
    14401 tccaccgcat gaattaaaaa aaggaaaaag gaatctctct attttgagct cttcaccgtc
    14461 tttgcaccgc ggagtatggc gatcttccct gtcctgacca gttcctttac cccgtactgg
    14521 cgcaacagct tctcgagggc atcgatcttc tcggtgtccc ccgccaccga gaggaccacg
    14581 gtcctgctgc ccacgtcgat gatctgtgca cggaagatgc ttgcgatctg catgatctct
    14641 gcccg
//
LOCUS       DAOQYR010000054        14487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_4633_length_14487_cov_7.262749, whole genome shotgun sequence.
ACCESSION   DAOQYR010000054 DAOQYR010000000
VERSION     DAOQYR010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 14487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14487
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4633_length_14487_cov_7.262749"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="PKM87_07465"
     CDS             <1..1434
                     /locus_tag="PKM87_07465"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3344 domain-containing protein"
                     /protein_id="HNQ29942.1"
                     /translation="LYQGYTYDQTPGGEPAWTMNFNGQEVTPDMAYTDRKGYGSYNNP
                     SGLLVYNVTGLFEETGNTLTVTPGAGNNNGLYGSLLVVVYDDESEPMRKIALNEECDM
                     LYAGAARFASDEEATAYAPFGSAVTSDIEEANVLSILFSANEAGKSAFIFNGQDYGDF
                     SSGYLAGPQVTLKEFDVTDEVTSGENTAQFRSVAVGASGDNMVAAGAIFVTGYTEAPP
                     VAAFSASPLTGTAPLQVQFSDQSTGTVTEWAWESRPERGVWAQFSTAPDPSYTFADAA
                     TYDIRLTVRGPGGESTDEKSAYISVLPPGGLRADFSATPTSGVAPLSVGFTDQSTGTV
                     TLRTWDFNNDGRWDSISRNPTYRYWNPGVYTVRLGVSGPEGSDGMIKQNYITVTAPPK
                     PPVARFTQDTRFGPAPLTVRFTDLSTGSPESYLWTFGDGQSSTDPSPVHTYQAPGLYR
                     LTLRVENEGGSSIANGFVYVIRNIRQY"
     gene            1946..2095
                     /locus_tag="PKM87_07470"
     CDS             1946..2095
                     /locus_tag="PKM87_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29943.1"
                     /translation="MSFIVPVFRISHRTRFRMRQIEKDEWADIFTVDLSRQGERLLGS
                     IPRHK"
     gene            complement(2129..4174)
                     /locus_tag="PKM87_07475"
     CDS             complement(2129..4174)
                     /locus_tag="PKM87_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative cobaltochelatase"
                     /protein_id="HNQ29944.1"
                     /translation="MLPPSRRTLLPFTAIVGQEKMKKALLLNAINPRIGGVLIRGEKG
                     TAKSTAVRALAALLPEIEVVKGCPFQCNPKNEREMCEHCTEKVSGGIALESTTRRVRV
                     IDLPLGVTEDRLVGTIDIEQAIREGTKAIEPGILAAVNRGILYIDEVNLLDDHIADVL
                     LDSAALGINVVEREGISFSHPARFILIGTMNPEEGEIRPQLLDRFGLQVAVEAIDDPE
                     QRMEIVRTAEAFDADPAGFSKRFESAQETLREMIIRGREILPTVTVPEDILRALVHAC
                     IELGVKTHRAEIAVARAARTIAALDGRCEVTLADVREAMELALPHRMRRKPFEEPRLD
                     PDRLDSAMKNAEQNNQQEQEPHSSDLPKHQEEPFPQTPSDQTDEGKIQSPREMESKAQ
                     ERVFGIGDPIDTHRIALAGDRDREKRKRLNGRRIETYSSGKNGSSITSRLPTGTMDLA
                     LGATIRAASPHQRARSRGDLAVSICEGDIREQVRVGKVSASCIFLVDASGSMGAMKRM
                     EAAKGAILSLLLDSYQHRDRIGMVAFRGECAEVLLPLSHSVDLARKSLADLPTGGKTP
                     LTDGIWKAIEVLDRERRKNGEAVPVLVLISDGRANVSRRGDIRTEILDAAHELRSRGV
                     HTVIIDTEETGSNFLKMQVGYCREIAEESGGRYYAVSDLKTEVLGEITRNEFARIFS"
     gene            complement(4505..4942)
                     /locus_tag="PKM87_07480"
     CDS             complement(4505..4942)
                     /locus_tag="PKM87_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNQ29945.1"
                     /translation="MTKKHLRETVIRIKLKEIAEGVSAVQQHLPSTGEEFRNLGLLKD
                     GIYKRIEFSIENVFDICAIINTDLALGVPGDDEDILVHLVDHGLISRSMRDKIHGMRG
                     FRNIVVHRYGTIDDVLAFRLLTGNIGDFLLFTEEIGKVLARYR"
     gene            complement(4939..5292)
                     /locus_tag="PKM87_07485"
     CDS             complement(4939..5292)
                     /locus_tag="PKM87_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNQ29946.1"
                     /translation="MEQLDGYDHVQFVILFGSAGENRMTGESDIDLCLYYDGDPAEAL
                     QFRHAILSALPGIRYDIKIFQALPLYVRVEVLKGTPVFVRDQVFLYEKAVETLRDFDN
                     FKHRLYDYTGQAAIS"
     gene            complement(5712..6500)
                     /locus_tag="PKM87_07490"
     CDS             complement(5712..6500)
                     /locus_tag="PKM87_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29947.1"
                     /translation="MKNDVAIAPVIAAMLILAVVVTLFSIYNATYLPGLKQSSEIQHL
                     HEVEEGVLKFGSAIEEAASLKRNISLSQQIPLGGGDIVLNSLKSGGSLHVQQENEPYL
                     TVYNNSLEVYSLTLINFSYWTVNNFWVDQGYTWQYGYVNVTRDPESGGTWLQHVTMDD
                     VNKSIDSFGQALIIEYQDGPDITIEAVNFLAGEKSFTSGNGYGKLKLNATRNKVTFKS
                     EDDCLIKLSVKDTVFSECLNEYNGRDYPDKNVTIILTNITVSTS"
     gene            complement(6490..6948)
                     /locus_tag="PKM87_07495"
     CDS             complement(6490..6948)
                     /locus_tag="PKM87_07495"
                     /inference="COORDINATES: protein motif:HMM:NF019407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HNQ29948.1"
                     /translation="MCCKEKRNRDDAVSELVGEMLMLTIVLILLAVFSATASNFLPQP
                     RDPTVTIMVDGSKNATDPVNFIDICLYHKGGDWIKKSDLTVIISDEKPTISKYPYDNE
                     SFVLEPDSQSFDLGGRIVIVHVPKALAPYEVQLVTPRAVVFSGEVLIHEE"
     gene            complement(6938..8749)
                     /locus_tag="PKM87_07500"
     CDS             complement(6938..8749)
                     /locus_tag="PKM87_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNQ29949.1"
                     /translation="MPLYEGFGIIRDRLFDREGFSRNLRGARIRKSYSEYTREILVQT
                     GLAVAGAVTALVVFHIFSIRFTILKPYPLWVQDALVLAVPGIMVFLVLYFQPVLAMMG
                     RKARIEMDLPYAITYMQALSTTLTLYNAIRGVYEQGDLYGEVSKEFGMIVRDVEVFGD
                     DLVTAIRNLARTTPSDNLKKLLDDLILMFESGGDLPGFLASRSSHYREVAEKELEMSL
                     KTMEIMAEVYVTAFVAAPIAVIIMVVAENMTGQSTLSALMPYFFIFLPLGAAAMVWIL
                     SLVVPGENVQITHKESRETEYGTGIAVIEGEKPDREFSRQIAARRKLLRVAAMIRNPI
                     RTYMSDYRFGIVIGTLFGSVIALMGMAGMFQDVFPRYPGEIMICLMTVAMLAPLMVAY
                     EIRRFYIHQVEAQVPDFLRELLDLKDIGMTLQGAVRLISESKIGLLSSELKLVSKDVQ
                     WGTSITSALVRLEERIGVLVIKRVISLIIRASEVTDYIRDILIIAISDMEHYLKMKRE
                     RYTVSFAYIMIIYLSFGIFLYTAYQLNVSFISSFEKLNTNIDISGNVLDMFRMSIILG
                     IFSGIMAGQLSAGSVLSGFKHVIVFLVAAVGLFVYVL"
     gene            complement(8736..10205)
                     /locus_tag="PKM87_07505"
     CDS             complement(8736..10205)
                     /locus_tag="PKM87_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HNQ29950.1"
                     /translation="MSGEGKIRDRYWLRPGFCSASIIENELFDLLYTVIEPQVSMRER
                     ILLEEVHDYLRDVLIYDTITRKDDTRLSFDDVRRGISRFMPGLPDDRASVIYYYLERN
                     LQGYGPIDPLMHDRFIEDISCNGYTSPVFIYHAEYGSIPTSIRFTAQELNRYVLKLSQ
                     KADRQVSISSPLLDAPLPDGSRAQITYTDVISSKGSSFTIRRFKSDPMTPVDLIRYGT
                     YDPEILAYIWLAVENRKSMIIVGGTASGKTSTMNAISYFIPLTAKIVSLEDTREIQLP
                     HKNWLPTKTRETSAQSGKGDIDLFSLLRASLRQRPEYIIVGEVRGPEAQTLFQAMNTG
                     HTTYSTLHAGNVDESINRLVNPPINVPRAMFGALDLMVVQLLQYQDGRAIRRCLSLNE
                     ISFDKATNIQSQDLYRWNPRSDTFERNYSRSRVLDSIAYSRGWSSAEVEEQLSLRAHT
                     LSLMARKKIVRSDQVSGIFHLMRKSAFYSGDEEADDAAL"
     gene            complement(10364..12067)
                     /locus_tag="PKM87_07510"
     CDS             complement(10364..12067)
                     /locus_tag="PKM87_07510"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ29951.1"
                     /translation="MVPFFHETYSLSWNNIALLARPLSPPFIIDFTVEADSENPYEAR
                     VLLTVRDNATHEVIAEDGYNGEYSSESEKRIVIREDGEYHINLYGYHATVDLSLKEGI
                     PDGWVVVPETFIPESVHSVPTSGKDDPGTSRVAPGAEILNEKGESLLIEGNYPGALGY
                     FDQAIAADTGYVPARVNRGIVLLSLGKAPEALLSFEIVIDRDPDNAQAWIYRGDALTS
                     LGRQDEARESYQQAGKIEPGNSLVQERLTRMKERDSLDGLAGIFQISFGVVLGLITLS
                     VITLFMLLKKRGKKESSLHPVHTGQVPEHSWNTRSVKGKLLNGLVSLFHRKAPGENLH
                     NPAPDRSAPPPMGNPGPLDTRGSVSTNGNTIRSGTENRNQIIQGFDRVLAGSGIDSSG
                     FRGLSHYAMGDYKDAFEAFTEYNQVDRDNPKIFALQASSLLKLERMEDALQVCETALR
                     DGKGTFEIRKIQCNILERMGRWEDTLRACDEALTLNPQSVEICSLKARALYCLNRNQE
                     ALQSCEFALRIDPTSTEILQQKTLLLADIGEVSDKGVFFEPAPNEGKDGSGIGKGGWL
                     S"
     gene            complement(12621..13208)
                     /locus_tag="PKM87_07515"
     CDS             complement(12621..13208)
                     /locus_tag="PKM87_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HNQ29952.1"
                     /translation="MDFHYSDTTLVIEGPFLAVSTGVGGDLKQVDALFNHTVPRDWHD
                     DSPGLLLSDIARKEGIYGDYFGLLTAVPMTSLLVCRSGFLTVFITSGISNHTINIIAI
                     SSEGMSKNALLESVATISSAKTQALLETGRGIVATPTDAVIMACEGEEIHQYAGIVTA
                     VGSRLAECVRRGVPIALSRYENGELSPATINLDIP"
     gene            13436..14137
                     /locus_tag="PKM87_07520"
     CDS             13436..14137
                     /locus_tag="PKM87_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HNQ29953.1"
                     /translation="MTKEDAVSPVVGVMLMLVVTIIIAAVVSGFAGGLAGGTKTAPSA
                     SIDVKIYSLENYGGMPPWGDGYYCPGMVIEHLSGDVLPTKDLQIVTYFTDRSSGEIIK
                     GNLTGEVAVGGDAGWSSWTATQYCGVLFLNDQNRFGDDTIQDSDSGNDNWFGNASAIF
                     RPGDILCDPHSFAGNYNDNDGPDAPHVNPAMNSLFGINCSDRSTGFVPGAIVDFKILH
                     IPSGKYIFDKEVSIQ"
     gene            14134..>14487
                     /locus_tag="PKM87_07525"
     CDS             14134..>14487
                     /locus_tag="PKM87_07525"
                     /inference="COORDINATES: protein motif:HMM:NF019407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HNQ29954.1"
                     /translation="MRIKEESAVSPVVGVMLMLVVTIIIAAVVSGFAGGLGGGTGKAP
                     QASIDCTVDIVHYQFIFEHKGGDAFALKDVEVILATEDTKTTLTIADIGGNVVAFKEV
                     GASDNMIAAGDKFILE"
BASE COUNT         3300 a         3867 c         3873 g         3447 t
ORIGIN      
        1 ctctaccagg gatacaccta tgaccagaca cccggcggag agcctgcgtg gaccatgaac
       61 tttaatggcc aggaggttac tcccgatatg gcctacacag accggaaagg atacggcagc
      121 tacaacaacc cctccggtct ccttgtctac aacgtgaccg gcctcttcga agagacaggg
      181 aacaccctca cagtcacgcc gggagcaggc aacaacaatg gcctctatgg cagccttctc
      241 gtggtcgtct acgatgatga gagcgaaccg atgagaaaga ttgccctcaa cgaggagtgt
      301 gacatgctct atgcgggtgc agcccggttt gcatccgacg aagaagcaac agcatatgca
      361 cctttcggaa gcgcggtgac atccgacatt gaagaagcga acgtcctttc catcctcttc
      421 tctgcaaacg aggcaggaaa gagcgcgttc atcttcaacg gccaggacta cggcgacttc
      481 tcgtccgggt acctggcagg gccgcaggtg accctcaaag agttcgacgt gaccgatgag
      541 gtaacctctg gcgagaacac ggcgcagttc cgcagtgttg cggtgggagc aagcggtgac
      601 aacatggtcg ccgccggcgc cattttcgtg acaggataca ctgaggcacc accggttgcc
      661 gcgttctctg ccagccccct gactggtact gctccactcc aggtgcagtt ctctgaccag
      721 tcgacgggga ccgtcaccga atgggcatgg gagtcccgcc ccgagagggg ggtatgggca
      781 cagttctcga cagcccccga tccctcgtat acgttcgctg atgccgcaac ctacgacatc
      841 cggctcaccg tccgcgggcc gggaggggaa tcaaccgatg agaagagcgc atacatctct
      901 gtcctcccgc cagggggcct gcgtgcggac ttctccgcca ccccaacttc aggagttgct
      961 ccgctctctg ttgggttcac tgaccagtcc acggggactg tcacactacg gacgtgggac
     1021 ttcaacaacg acggccgctg ggacagcata tccagaaacc ccacgtacag gtactggaat
     1081 cccggcgtct acacagtgcg gctcggggtg agcgggcccg agggatctga cgggatgata
     1141 aagcaaaatt acatcaccgt gaccgcgcct ccaaagccgc cagtggcacg gttcacgcag
     1201 gacacgaggt tcggccctgc acccctcacg gtccggttca ctgacctctc gacaggatct
     1261 cccgaatcat acctctggac gtttggcgac gggcaatcct cgacggaccc aagccctgtc
     1321 cacacctacc aggcgcccgg actgtaccgg ctcaccctgc gggtggaaaa tgagggcgga
     1381 agctccattg cgaacggttt tgtctacgtg ataagaaaca tcaggcaata ttaatccttt
     1441 tttcgccgca ttcacaccgc agggtggccg gcccgccttt cccggccttt cccctccacc
     1501 tcttctcctc ttgtatctct cgaactcaaa taattaggaa taaatgctac caaaataata
     1561 attcttaata ccaatgtgga agattatgat ctggcagaga ctcttcaggt ctgctgctgt
     1621 caccggtact gcaacatcgg ttgagtgggt cctggcatac aaggcccacg gtcatcacac
     1681 tatcaggagg gccatgatca ccagtggcag cacacttacg gttttccctc gaagcaatca
     1741 gggggagggc agcatacgat atacctcaaa ggagaagagt atcccgggga catccgcccg
     1801 atccccgtat tcccattttc ttccggaatg cgatgccggg agagggcatc caccccataa
     1861 aggagtacat ccacccgaac ggtgtgggaa aataagaaaa gccgggtcat tgccgccccg
     1921 tgaccatgga acaaggatgg tctgaatgtc gtttatcgtg ccggtcttca ggatctctca
     1981 ccgcaccagg ttcaggatgc ggcagatcga gaaggatgaa tgggcagata tcttcacagt
     2041 cgacctctcc cgccagggag agaggctgct tgggtctatt ccgagacaca agtgaaacga
     2101 ttttcttttc gcttccctgc atccccggtc aggaaaagat ccttgcaaat tcgttccggg
     2161 tgatctcccc aagaacctct gttttcaggt cgctgacagc gtaatatctg ccgccggact
     2221 cttctgcaat ctccctgcag tatccaacct gcatcttcag aaagttggag ccggtctcct
     2281 ctgtatcgat aatcacagta tgcacgcccc gggagcggag ttcatgggcc gcatcaagaa
     2341 tctctgtgcg gatgtctcct ctccgtgata catttgccct tccgtcggat atcagcacca
     2401 gcactgggac tgcctcgccg ttcttccgcc gttcacggtc aagcacctcg attgccttcc
     2461 agataccatc ggtaagcgga gtctttcccc ccgtgggaag gtctgcaagg ctcttcctgg
     2521 caaggtcgac gctgtgggag agcggcagca gcacctctgc gcattctccc ctgaacgcca
     2581 ccattccgat ccggtcgcgg tgctggtacg agtcaagcag gagggagagg atagccccct
     2641 tggctgcctc cattctcttc attgcaccca tcgaaccgct cgcgtcgaca aggaagatgc
     2701 aggaggcaga gacctttccc accctgacct gctccctgat atccccctca caaatagaga
     2761 ctgcgagatc tcccctggat ctcgctctct ggtggggcga ggccgccctg atggtcgcgc
     2821 caagggcgag gtccattgtc ccggttggca gccttgaggt gatggaggag ccgtttttcc
     2881 cggaggaata tgtctcgata cgcctcccgt tcaaccgctt cctcttctcg cggtccctgt
     2941 ctccagcgag ggcgatcctg tgggtatcga tcgggtcccc gatgccaaag actctttcct
     3001 gcgccttgct ttccatctcg cggggcgact gaatctttcc ctcatcagtc tggtctgaag
     3061 gagtctgagg gaatggttcc tcctggtgct tcggcagatc ggaggagtga ggctcctgct
     3121 cctgctggtt gttctgttcc gcgttcttca tcgcggagtc gagccggtcg gggtcgagcc
     3181 tcggctcctc gaagggcttc cggcgcatcc ggtgggggag tgcgagctcc atcgcctccc
     3241 tgacatcggc aagcgtgact tcgcatctcc cgtcgagtgc ggcaatggtc cgcgctgccc
     3301 tggccaccgc gatctcggcc ctgtgtgtct tgaccccgag ctcgatgcag gcatgcacaa
     3361 gtgcccgaag aatatcttca gggaccgtga cagtaggcag gatttccctt ccccggataa
     3421 tcatttcgcg gagagtctcc tgtgctgatt caaatctttt cgagaacccg gccggatcgg
     3481 catcaaaggc ttcggcggta cggacgattt ccatcctctg ttccgggtca tcgattgctt
     3541 cgaccgcaac ctgaagaccg aacctgtcca ggagttgcgg ccggatttct ccttcctcgg
     3601 ggttcattgt cccgatgagg atgaaccggg ccgggtggga gaaggagatc ccctcccgct
     3661 ccacgacgtt gatgccgagc gccgccgagt cgaggaggac gtcggcgatg tggtcgtcga
     3721 ggaggttcac ctcatcgatg tagaggatcc cgcggttcac ggcggccagg attccgggct
     3781 cgatagcctt cgtcccttcc ctgatggctt gctcgatgtc aattgtcccg accagacggt
     3841 cctctgttac gccgagcgga aggtctatca cccgaactct ccgagtggtg ctttcaaggg
     3901 caataccacc ggataccttc tccgtgcaat gctcgcacat ctccctttcg ttcttgggat
     3961 tgcactggaa cggacagccc ttcaccacct ctatttcagg caggagagcg gcgagtgccc
     4021 ggacagcagt ggacttcgcg gtcccttttt ccccgcggat cagcactccc ccgatgcggg
     4081 ggttgatcgc gttgagaagg agcgccttct tcatcttctc ctgtcctacg atagcagtga
     4141 atgggagaag cgtgcggcgg gaggggggta acatgattga aataatattg taatcatttc
     4201 ttaaatatat tatttcttta gtctttgagt aataaacatc caaaaatttt ttccactccg
     4261 tgcgaaaatt tattaacggc tagatcatct ggtttcttta gagggacaat tccattcgcc
     4321 tgactccccc atttaccatc tcccattctt cccaattttt tcatgtgaaa ttcaaatgat
     4381 tctcagaata aaataaaaaa atttcggcac agatgatctg aaataattac acggatttcg
     4441 aacccataag tgcgcattga atcccggctt ttgatactgc aggaaaccct tcagggttat
     4501 cctgctaccg gtaccgggca agaaccttcc ctatctcctc agtaaacagg agaaagtccc
     4561 cgatgtttcc ggtcagcagc cggaatgcga gaacgtcatc gatcgtcccg taccggtgca
     4621 cgacaatatt ccggaatcca cgcatcccat ggattttatc ccgcatggac cggctgatga
     4681 ggccatgatc cacgagatgc accaggatat cctcgtcatc gccgggaact ccaagagcga
     4741 gatccgtgtt gatgatggcg cagatatcaa aaacattctc aatggaaaat tcgattctct
     4801 tgtagatccc gtcttttaaa aggccaaggt tccggaattc ctcccccgtt gagggcagat
     4861 gctgctgaac tgcggagact ccctcggcaa tctcttttaa cttgatacgg atgaccgttt
     4921 cccgcaggtg ttttttggtc atgatattgc agcctggccg gtataatcgt acagtcggtg
     4981 tttgaaattg tcaaaatccc ggagcgtttc gaccgccttt tcgtataaaa atacctgatc
     5041 gcggacaaaa acaggtgtcc ctttcagcac ctcaaccctt acatacaggg gaagggcctg
     5101 gaagatcttg atatcatagc gtatcccggg aagagccgac aggatcgcgt ggcggaactg
     5161 gagcgcctct gccggatcgc cgtcataata caggcagaga tcgatatccg attccccggt
     5221 catacggttt tcccctgccg aaccaaagag gatgacaaac tgtacgtggt cgtaaccatc
     5281 aagctgctcc atcgttttca gagctgctgc tgtgtacgca ctgacgtcct gttcaggtac
     5341 agaagatctc cggcctgctg gcacaacctc ttctcctcat gaacactctt tcctgcattc
     5401 catttattct ttgcccggct cacaaatcca acctgaaacc tgccgggacc tcccggtgtg
     5461 gagaaaaatc ctggaaaaga gtcaacgggg gagtccttac tgggctgttg cagggaaaac
     5521 cggttatttc gatccgatct gcaaggagcc ggacgtggaa tgtgtctccc cgccgtttga
     5581 gctgttgaac gcagagacgg cagggattgc gaagggggat gaagggcagt tttccctgaa
     5641 ttcacatcac ttcctggcgg gggtttctct ttccgggatg caacccgtag tataggccat
     5701 cccatttctc atcatgaagt tgataccgtg atgtttgtca ggatgatggt gacattctta
     5761 tctgggtaat ccctaccatt gtattcattc aggcattcgg agaagacagt atctttaact
     5821 gataatttta tgagacaatc atcctcactc ttgaaggtca ctttatttcg cgtagcgttt
     5881 agtttgagtt ttccataacc gttcccgctg gtgaatgatt tctcacctgc aagaaaattg
     5941 accgcctcga ttgtgatgtc tggaccatcc tgatattcta taatgagtgc ttgtccaaag
     6001 gaatcaatag acttgttcac gtcatccatt gtcacatgct gaagccatgt gccacctgat
     6061 tccgggtccc gcgtgacgtt tacatatccg tactgccagg tgtatccctg gtcgacccag
     6121 aagttattca ctgtccagta gctgaaatta atcagtgtga ggctatagac ttccagacta
     6181 ttattatata ccgtgagata cggctcattt tcctgctgca catgaagact ccccccggac
     6241 ttcagggagt tgaggacaat atcccctcct ccgagcggga tctgctgtga gagcgagatg
     6301 ttccgtttca gggatgcagc ctcctcgatg gcagagccga acttgagaac tccttcttca
     6361 acctcgtgga ggtgctgaat ctctgatgac tgcttcagtc ccgggaggta tgttgcattg
     6421 taaatagaga agagggtgac aaccactgca aggataagca tcgcagcaat gacgggtgcg
     6481 atagcaacgt cattcttcat gaatcaacac ctcccctgag aagaccactg cacgcggtgt
     6541 gaccagttgg acttcatacg gtgccaatgc cttgggaaca tggacaatga ctatccggcc
     6601 gccaaggtca aatgactgcg agtcgggctc aagaacaaac gattcattgt cgtatggata
     6661 ttttgagatg gttggttttt catctgaaat gataacggtc agatctgact tctttatcca
     6721 gtcacctccc ttgtggtaga gacatatgtc aatgaaattg acaggatctg tggcattttt
     6781 agatccgtca accattatag tcacggttgg atcccgtggt tgaggcaaaa aatttgaggc
     6841 cgtcgcagag aatacggcaa gtaggatcag cacgatggta agcatcagca tctcgcccac
     6901 gagttcggaa accgcgtcgt ccctgtttct cttctcctta cagcacatac acaaacaacc
     6961 ccaccgctgc gacaagaaat acgatcacgt gcttgaaccc cgaaaggacg ctccctgcag
     7021 agagctgccc ggccataatc ccggagaaga tcccgaggat gatggacatc cggaacatat
     7081 cgagcacgtt tcccgagata tcgatattcg tattcagttt ctcaaaagag gagatgaagg
     7141 atacattcag ctgataggcg gtgtacagga atattccgaa tgaaaggtag atgatcatga
     7201 tataggcaaa agaaacagtg taacgctccc tcttcatctt caggtagtgt tccatgtcgc
     7261 tgatggcaat gatcaggata tcccggatat agtccgtgac ttcgctcgca cggatgataa
     7321 gggagatgac ccgctttatc accagcaccc cgatgcgttc ttcgagtcgg accagtgccg
     7381 acgtgatact ggtcccccat tggacgtcct ttgagacgag cttcagctcg gagctcaaaa
     7441 gaccaatttt tgattcggag atgagcctca ccgccccctg cagcgtcatc ccgatatcct
     7501 tcaggtcaag gagctcccgg aggaagtccg ggacctgggc ttcgacctgg tggatgtaga
     7561 atctgcggat ctcgtatgcc accatcaggg gggcgagcat cgcaaccgtc atcaggcaga
     7621 tcatgatctc tcccggataa cgggggaaga cgtcctggaa catgccggcc attcccatca
     7681 gcgcgatcac agaaccgaac agggtgccga tcacgatgcc gaaccggtaa tcggacatgt
     7741 acgtgcggat ggggttccgt atcatcgccg caacgcggag gagctttctc cttgccgcga
     7801 tttgccggga aaactcccgg tcaggcttct cgccttcaat gaccgcaata ccggtgccat
     7861 actctgtttc cctgctctcc ttgtgggtga tctgtacatt ctcacccggg accacgaggg
     7921 agagtatcca gaccattgcg gccgccccga gggggaggaa gatgaagaag tagggcatca
     7981 gggctgagag cgtggactgg ccggtcatgt tctcggccac gaccatgatg atcactgcga
     8041 tgggggcggc cacaaacgcg gtgacgtaga cttctgccat gatctccatc gtcttcaggg
     8101 acatctcgag ctctttttcg gcaacctcgc ggtagtggga ggaccgcgat gcgaggaacc
     8161 ccgggaggtc gcccccgctc tcgaacatca ggatgaggtc gtccaggagt tttttcaggt
     8221 tgtctgaagg tgtggtccgg gcaaggttcc tgatagcggt caccaggtca tcaccaaaga
     8281 cctccacatc acggacgatc atcccgaact ccttcgagac ctccccatag aggtcgccct
     8341 gctcatagac tccccttatc gcgttgtaga gtgtgagggt tgtggagagc gcctgcatgt
     8401 aggtgatggc ataggggagg tccatctcga ttcgggcctt ccgccccatc atggcaagca
     8461 ccggctggaa atacaatacc aggaagacca tgattcccgg tactgcaagg acgagggcat
     8521 cctgcaccca gagggggtag ggtttcagta tagtaaaacg gatggagaag atgtggaaca
     8581 ccaccagtgc agtgactgct ccggcgacgg caaggccggt ctgtacaagg atttcacgtg
     8641 tatactcgct gtaggacttc ctgatccggg cgccccttag gttccgggag aacccctcgc
     8701 ggtcgaagag gcggtccctg ataatgccaa atccttcata aagcggcatc gtcagcctcc
     8761 tcatctccgg agtaaaatgc tgatttcctc atcaggtgga agatcccact cacctggtcg
     8821 gatcggacga tctttttcct ggccatgaga gagagtgtgt gggctcggag agacaactgc
     8881 tcttcaactt cagcacttga ccatccacgg gagtacgcga tgctgtcaag gactcgggat
     8941 cgggaatagt tcctctcgaa ggtatcggag cgtgggttcc accggtaaag gtcttgggac
     9001 tggatattcg tggctttatc aaacgagatc tcgttgaggg aaaggcagcg gcggatggcc
     9061 ctgccatcct ggtattgcag gagctggact accatgaggt caagtgcccc gaacatcgct
     9121 cgagggacgt tgatgggcgg gttgaccagt cggttgattg actcatcgac gtttcctgca
     9181 tgcagtgtgg agtaagttgt gtgccccgtg ttcatggcct ggaagagcgt ctgggcctcg
     9241 ggccccctca cctccccgac aatgatatac tcgggacgct gccggagcga cgcccggagg
     9301 agggagaaga ggtcaatatc cccctttccc gactgtgcgc tggtctcccg ggtctttgtc
     9361 gggagccagt ttttgtgggg gagctggatc tcacgggtgt cctcgagcga gacgattttt
     9421 gcagtgaggg ggataaagta ggagatagcg ttcatcgtcg aggtctttcc gctcgccgtc
     9481 cctccgacga taatcatgct tttgcggttc tccacggcga gccagatata ggcaaggatc
     9541 tcggggtcat aggtcccgta gcgaataaga tccacagggg tcatcgggtc gctcttgaac
     9601 cggcggatgg tgaacgagct ccccttcgag gatatcacat cggtgtaggt gatctgtgcc
     9661 cgtgaaccgt caggaagcgg ggcgtcaagg agtggagagg atatggagac ctgcctgtcc
     9721 gccttctgcg agagcttgag gacataccgg ttcagttcct gtgccgtgaa cctgatcgat
     9781 gtggggatag aaccatactc tgcatggtag atgaaaaccg gcgaggtgta gccgttgcag
     9841 ctgatatctt cgatgaagcg gtcgtgcata agggggtcga tggggccata gccctgcagg
     9901 ttccgctcaa ggtagtagta gatcaccgag gcacggtcat ccggcagtcc cggcatgaac
     9961 cgggagatcc ccctcctgac atcgtcgaac gagaggcggg tatcatcctt ccttgttatg
    10021 gtatcgtaga taaggacatc ccggaggtaa tcgtgaactt cctcgagaag aatacgctcc
    10081 cgcatgctga cctggggctc gatgacagtg tagaggagat cgaagagctc gttctcgatg
    10141 atggatgcag agcagaaccc cggacgcagc cagtaccggt cacggatctt tccttctcct
    10201 gacattgttc ctgtcggggg aagaatggga atgggagtta ccggcggctc ctttccggac
    10261 tccgggcctt tccctgatcc ggacagacat tcctgaattg acctggcaac tctctcctta
    10321 ctatcccata gagcctttcc tttgattgta tctgaagaca ccatcaggag agccaccctc
    10381 ccttccctat tccagacccg tctttccctt catttggggc tggctcaaag aatactcctt
    10441 tatcagacac ttctccaata tcagcaagga ggagagtctt ctgctgaaga atttccgttg
    10501 acgtgggatc tatcctaaga gcgaattcac aggactgaag agcctcctga ttcctgttca
    10561 ggcaatacag tgcccgtgcc ttcagagaac atatctcgac tgattgggga ttgagagtga
    10621 gggcttcatc gcatgcgcga agggtatctt cccatctccc cattcgctca aggatgttgc
    10681 actggatctt ccggatctcg aatgtgcctt tgccgtcgcg gagggctgtt tcacacacct
    10741 gcagggcatc ctccatcctt tccagtttga gaagcgatga agcctgaagg gcaaaaattt
    10801 tcgggttgtc cctgtcgact tggttatatt cagtaaatgc ctcgaatgca tccttgtagt
    10861 ctcccatggc ataatgggag agtcccctga accctgatga atcgatgccc gaacctgcaa
    10921 gaacccgatc aaaaccttga ataatctggt tcctgttctc cgtaccggaa cgtattgtgt
    10981 ttccattggt ggacacgctc ccacgagtgt ccaatggccc agggttgccc ataggtggtg
    11041 gagcgcttcg atccggggcc ggattgtgaa gattctcccc cggtgctttc ctgtggaaca
    11101 aggatacgag tccgttgaga agttttccct tcacggaccg cgtgttccag gaatgctccg
    11161 gaacctgtcc ggtatgaact ggatgaaggg aggattcttt ctttcccctt tttttaagca
    11221 gcataaaaag cgtaattacg gacagagtga tgaggcccaa cacaacacca aaggaaattt
    11281 gaaaaattcc tgcaagtcca tccagggagt ctctctcctt cattctggtc agcctttcct
    11341 gcaccaggga attgccgggc tcaatttttc ctgcctgctg gtaggattcg cgtgcttcat
    11401 cctgccttcc cagtgaagtg agcgcatcgc cccggtatat ccatgcctgt gcgttgtcgg
    11461 ggtcccggtc tatgacaatc tcaaaggaga ggagtgcttc cggggccttt cccagtgaga
    11521 gaagaacaat tcccctgttt actcgggccg gcacatatcc cgtgtcagca gcgattgcct
    11581 gatcgaaata cccgagagct cctgggtagt tcccttcaat cagaagcgat tccccttttt
    11641 cgttcagtat ttctgcccca ggcgcaacac gactggtgcc ggggtcatcc tttccggatg
    11701 tggggaccga atgaacactt tcaggaatga atgtttcggg gacaacgacc catccatccg
    11761 gaattccttc tttcagggac aggtcaacag tggcatggta cccatacagg ttgatgtgat
    11821 attctccatc ctccctgatg actatcctct tttccgattc agaactatat tccccgttgt
    11881 atccatcctc tgcaatcact tcatgcgttg cattgtccct gaccgtcaag agaacccttg
    11941 cttcatacgg attttcggaa tcggcctcga cagtgaaatc gatgataaaa ggtggagata
    12001 aaggtcgggc aaggagggca atgttgttcc atgagagact gtaagtctca tggaagaaag
    12061 gaaccaccgg atacgccaga gggcggggga aaagatcctc gggctggtca agatcccttc
    12121 tagttacatt actggcgatt gaaggaggga ttggatcggt ataggcggcg aatcctttgg
    12181 atacccccga tatggattga tcgggaacgt tcactgtgtg tgaaccggcc atttgcgcag
    12241 atccaggtga ggaagcccgg gtcgccggtt gattactcgg ggcgtcatga gggtgtggtg
    12301 cagaatcctg ttgcagggcg ttctgggatc cgttgagttc tcccatctca atacatccac
    12361 aggacattga gaaaacgatg agaataaaaa tcgcggcggt cgcgattgaa gcggaaagag
    12421 aagggccgcc attttctccc tcttttttcc ggtgagatct tctttgagcg ttgtgtaaga
    12481 aaacgacatg attacttttt tccgcagtgt ccgggaacat gtgtccaatc ctggggaaaa
    12541 acgataacga tcctttatac aatcctatcc ggcccggaat caccgattgg attctaccag
    12601 gattcattca cgctcctctt tcagggtatg tccaggttga ttgttgcagg agagagttct
    12661 ccgttctcat accgggagag ggcgatagga actccgcgtc ttacgcattc cgcaagccgg
    12721 ctcccaaccg cagtgacaat tccggcatac tggtggatct cttccccttc gcaggccatg
    12781 atgaccgcat cggtgggggt tgcgacaatt cccctcccgg tctcaagaag agcctgcgtc
    12841 tttgcactgc ttatggtggc cacggattcg aggagggcat ttttagacat cccttccgaa
    12901 cttatcgcaa tgatattgat ggtgtgattc gagatccccg acgtaatgaa tactgtgagg
    12961 aagccggaac gacataccag gagcgatgtc atgggaactg cggtaaggag tccgaaataa
    13021 tcaccgtaaa tcccttcctt ccgggcaata tctgagagga gaaggcccgg ggaatcatca
    13081 tgccagtcac ggggaacggt atggttgaag agagcatcca cctgcttaag gtctccaccg
    13141 actcctgtag ataccgcaag aaatggtccc tcaatcacca gcgtggtgtc ggaataatgg
    13201 aaatccatat gaacgcacct gcacgatcgg caaagaccgg gcttttcgga tgaaatgaat
    13261 tggcatcaaa tcttttgcta cacattctcg tgttcgagat atataaaaca agttgatctt
    13321 gcttaaagca aatcaaattg atcaatttaa atagttataa ccaaaactca gttgcgttgt
    13381 gaagggacgg gccctccctg cacaactcca gaaaaatgga ggaatatgaa tggaaatgac
    13441 aaaagaagac gccgtatcac cggttgtcgg cgtcatgctc atgcttgtgg tgacgattat
    13501 tatcgccgcg gtggtaagcg gatttgccgg agggctcgct ggcggaacga aaacagcgcc
    13561 ttcagcatcg attgatgtga agatttacag tcttgaaaac tatggaggaa tgccaccctg
    13621 gggtgatgga tattattgtc ctgggatggt tatcgagcac cttagcggcg atgtgctacc
    13681 cacaaaagat ctccagattg ttacttattt cacagatcga tcttcgggtg aaatcatcaa
    13741 ggggaattta actggtgaag ttgctgttgg cggagatgct ggatggtctt cttggactgc
    13801 cacacagtat tgtggtgttt tgttccttaa cgaccagaat aggttcggtg atgatactat
    13861 ccaggactca gatagcggga atgataactg gtttgggaat gcctcggcaa tatttagacc
    13921 aggggatatt ctctgtgatc cacatagctt tgccgggaac tacaatgaca atgacgggcc
    13981 tgatgcaccg catgtgaatc ctgcgatgaa tagccttttc ggaatcaact gttctgacag
    14041 atcgacaggt tttgtaccgg gcgcaattgt cgattttaaa atcctgcata ttccaagcgg
    14101 gaaatatatc tttgacaaag aggtgagcat ccaatgagaa taaaagaaga atccgcagtc
    14161 tcacccgttg tcggagttat gcttatgctt gtagtgacca tcatcattgc agcagtagtg
    14221 agtggatttg ccgggggact tggcggtgga actggaaaag ctccccaggc atctattgat
    14281 tgtaccgttg acattgttca ttaccagttt atttttgagc acaaaggagg tgatgccttc
    14341 gccttaaagg atgttgaagt catcctcgca accgaagata ccaagaccac gctcaccatt
    14401 gcagacattg gcgggaatgt ggttgcattc aaggaggtcg gagcatcaga caacatgatt
    14461 gccgccggtg ataagttcat tcttgag
//
LOCUS       DAOQYR010000055        14368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_4686_length_14368_cov_8.087263, whole genome shotgun sequence.
ACCESSION   DAOQYR010000055 DAOQYR010000000
VERSION     DAOQYR010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 14368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14368
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4686_length_14368_cov_8.087263"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2055
                     /locus_tag="PKM87_07530"
     CDS             <1..2055
                     /locus_tag="PKM87_07530"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HNQ29955.1"
                     /translation="GKTVEEVVLKGTLVTAGRGRMIIGAVGDATQMGRIAADLAEGSR
                     PETPLQLKLRRLAGLVSRFGYLMAGLIIATLMIEGIFTGVFTGSPAAIAHYVLDSFMF
                     AVIIIVVSVPEGLPVSVTVSLALTMRKMTRAFSLVRRLIACETVGSVTVICTDKTGTL
                     TMNQMDVVASSVEFPDLQPGVPTTPNGWVTLNAAVNSTADLESREGRLVTVGNSTEAA
                     LLRWLHRAGLSYQEIRALHPPVTQELFDSNKKQMSTIVFLENTLFILVKGAPEIVATQ
                     CNPSPDLSHIQALAGRAMRTLAFAHAMLPAEGSDPPPLTWDGYVGIRDEVRPDVPDAV
                     RTCNEAGITVKMVTGDSPETAAAIARETGILTTGTVMLGSEFRSLPDGARPEAARRLE
                     VMARSEPSDKLLLVQALQGNGEVVAVTGDGTNDAPALRNADVGLAMGIAGTEVAREAS
                     DIILLDDSFPTIKNAVWWGRALFENIQRFLIFQLTINISAAMLSFLAPVLGYPPPFTI
                     IQLLWINIVMDSLAALALCSEAPHPALMHRKPIPRDAHVITPYMLRAILITAAVYVLV
                     GMLAVIFGLPFMETPEQQETAFFAAFVVAQVWNGINCRGINGVMPPFFRGNPAFFGIM
                     AGIVVIQVLIVQFGGALFSTVPLTAFQWVFIILCTSPVLLLWPVIRLSERYIRISRRS
                     GI"
     gene            2170..2874
                     /locus_tag="PKM87_07535"
     CDS             2170..2874
                     /locus_tag="PKM87_07535"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNQ29956.1"
                     /translation="MISPDLLSLSAILLNIEKVIPQLMETYGILIYAILFAIIFCETG
                     LVFAPFLPGDSLLFLVGFFAAGGGLNVWILLVLITIAAIAGDSLNYWIGNYFGDRLIH
                     GKRCLVDRRHVEVTRQYYHKYGKKTIILARFVPYIRSFAPFIAGIVKMNYPAFLSNNI
                     IGGILWVTGFISLGYLVASLPLFSENQDLFLWAILIISIGICLLMMYNFRKTIKECEI
                     PSDVLIEDLSRELRQD"
     gene            complement(3052..3390)
                     /locus_tag="PKM87_07540"
     CDS             complement(3052..3390)
                     /locus_tag="PKM87_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HNQ29957.1"
                     /translation="MDFALSTDIIEIFAFIFGIAGAALIIYGGLRAVIRVIYREVMKR
                     DISYNHIRRDFTSKIVFGLEFFIAADILTTFIAPTQEELILLAVVVIIRTVLGYFLAR
                     ETKEFPLEEE"
     gene            4400..5209
                     /locus_tag="PKM87_07545"
     CDS             4400..5209
                     /locus_tag="PKM87_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNQ29958.1"
                     /translation="MVPLDLFLLCMLGVLIGAISALLGVGGGFFYVPALVLLFGIDPR
                     IAVGTSLAIITCTTLSASIGYGLQGQVLMKSAACLALPGMAFAAFGALITAFLPSQYL
                     VALFCVVLLFMGIKMIYRKFPLVFPLLVGPCWKERFGKLHEKGEELFVYPVHLVTWGS
                     LAGFMSGLTGIGGGIVNVPALTIGGIPIHFAVATSAVVILITSLSATGTHLALGNLSI
                     PFFVAIAAGAVAGAQVGVRLSPKAPPRMLELAVGTLLIVVVAALVVNTHLI"
     gene            5324..6280
                     /locus_tag="PKM87_07550"
     CDS             5324..6280
                     /locus_tag="PKM87_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNQ29959.1"
                     /translation="MRSIEPHQAAIAVDLGATNIRAGLVREDGQILSLLIAPTPPHPE
                     DADVILRSLESLIARAVSATGMPPVAGMGISTAGPVDLQKGAVVNPPNIPLPFIPLAE
                     PLGERFGLPVRFMNDCHAGARGEAAFGCGRGVENFVYLTISTGIGAGVYEQGRLLFGE
                     DGNFAEVGHFVVETTWNLPCGCGGTGHWEGCGSGRFIPAFFLEWCRSSNHRSPFRNLS
                     DAAAIFAAAAGGDPVAGEFVSELGKVEARGISLLIAAYDPSLIVIDGSVARNNWHIFE
                     TSVLPHVRSVSGKIPQMRLSPLGGNAPLLGAAWSVFDRTPVP"
     gene            complement(6650..7489)
                     /locus_tag="PKM87_07555"
     CDS             complement(6650..7489)
                     /locus_tag="PKM87_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HNQ29960.1"
                     /translation="MSPGLPILISIPHGGVGIPEEVAGAVALDPPALSYYSDPASERL
                     FGFKAEVKAFASARVSRVIVDLNRPPYHLPPRRRDGVVKFQTSLGAPVWKEGMQPDIQ
                     AIHRLLLRHYFPYHAKLDSLLDSRKIAVAFDCHSMVPVGLPGQPDEGQPRPLICLGNN
                     GDRNGHPRRGALCTCPPEWIRQLAERFRDEFPGAGSVTLNHPFSGGFISNAHYWHKGI
                     PWIQVEVNRSLYEGGNSSPQQGALLDMQRMERLKEIIRGVLSMWYMEVVKEDSRTPPL
                     FQE"
     gene            complement(7490..8086)
                     /locus_tag="PKM87_07560"
     CDS             complement(7490..8086)
                     /locus_tag="PKM87_07560"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ29961.1"
                     /translation="MPPREILNTPRLWLRRFSIDDAQSVQEHADDMCIAAGVLEIPHP
                     YTLEDAEQWIGQHQGLWQRGEHYIFAITEKETTDIAGAASLRVNCAHSHAELGYWIGR
                     PFRCRGYATEAAGALLEFGFLGLGLHRIYARHLDWNTPSSRVLVRIGMHFEGAMKGHA
                     CKWGSFHDVHFYGIQADDFYPHYLDGSRNRVVPCKGGQ"
     gene            8252..8764
                     /locus_tag="PKM87_07565"
     CDS             8252..8764
                     /locus_tag="PKM87_07565"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HNQ29962.1"
                     /translation="MNTSILARKAVVIASLGAAAIISGYFGLALSLGTATVMDTRDIL
                     VVCAGVLAGPAGGALVGLLAGLPGPDPLSEVSLYMVGGLAVGFIARYCAANREWMPTA
                     ALGLGFGYVLSGAILMFTSWYDAVAALAFKSLIMLNICILVLSIIDSLDHRIFSWDRR
                     SPAPDSSGSR"
     gene            9437..9613
                     /locus_tag="PKM87_07570"
     CDS             9437..9613
                     /locus_tag="PKM87_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29963.1"
                     /translation="MTNDVKKTCDVCGRKAIGIQVLGCCGSVVCEVHAEDRLKHMSPG
                     EKIEWGSCFFYRYE"
     gene            complement(9757..11463)
                     /locus_tag="PKM87_07575"
     CDS             complement(9757..11463)
                     /locus_tag="PKM87_07575"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ29964.1"
                     /translation="MPPDKVHILLVDDRTDLISLFGDYLSTQKGYAVERVPSSREGIE
                     LLFARSYSLVISNDSSRDGSRLEAVASSRDIPLVRLKNERKRRGSSQFTLQAGDTIVV
                     EDSHQRMEFPLQGSSSLPALFSDLGRNLTQLMKGRNARRDFEASFGHPYDVISSAPVP
                     LSILTHPDISWMNEEMAHILGYSPSTMAGRPFHSILPREEDMRWFGELGRQSKGGAGW
                     VQREVVLRKRDGAHVPIRLKMRKLAGKEGEDMYLCICEDIRCHQSLLELQRSFDQKVM
                     EIESRYRAVFESMGAIVIRTDSDGNIVYCNPAAEMMFGFRSVDVAGSNVIGTLVPPGS
                     QSARDFMTMLSGCSRNADESSVIAVEHALPGLRRACIAWKMVGVERDGVVPDIVCVGE
                     DITDYSRPDSCGIRTDPWRTRLLKGTDIKRDVFDDVFSICVEISREGWEGKRVGTSFL
                     VGDASSVMAKSQPLILNPFNSMENEMRYVGNPGNKTCIKNFAMLDGGFIIGGDGFIVA
                     SGRQFLPGDVPLALPAGYGTRHRSVAAMTVVTGAVGIVVSETGGRLTVFRDGQSVKSL
                     IF"
     gene            complement(11530..12072)
                     /locus_tag="PKM87_07580"
     CDS             complement(11530..12072)
                     /locus_tag="PKM87_07580"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNQ29965.1"
                     /translation="MQDMSRIADEYSAYLKRLAGTLVEGERQSRALAGLIESSPAIHL
                     YGFGRSGTAGLAMAIRLRHFLAPEQQVFWLGDQVRDPIRQDDVVILFSKSGDRKEVIC
                     SLESARGAGARCVAITGDPASPLARGSSLTIILFPMDGGVIYGGGDFEIAAWYFQEIF
                     VTWYGRLHGIPPERVGERHI"
     gene            complement(12155..12226)
                     /locus_tag="PKM87_07585"
     tRNA            complement(12155..12226)
                     /locus_tag="PKM87_07585"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12191..12193),aa:Arg,seq:ccg)
     gene            complement(12363..13391)
                     /gene="hypD"
                     /locus_tag="PKM87_07590"
     CDS             complement(12363..13391)
                     /gene="hypD"
                     /locus_tag="PKM87_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNQ29966.1"
                     /translation="MALGKEISEKLRECADRDLSFMHICGTHEAAISRSGLRSMLPPG
                     LKIVMGPGCPVCITPQGEIDAALDLVGKGCIVATYGDLLRVPGSRGSLESCGGDVRVV
                     QGVHKAVEIARNTTREVAFISVGFETTAPTVAATLLTRPPENFSILSCHRLVPPAMKW
                     LLEQGEAKLHGFLLPGHVCTVVGYQDYEQFPVPQVVAGFEPEDILLGLLMLVEQVRAG
                     EAKVENAYPRAVTREGNTRARQLMYEVFTPVDAEWRGFPVIPRSGLGLRPEFEQYDAK
                     KKFDLTFRHVEKHSACICDKVLRGIAEPTDCRLFGKSCTPRTPIGPCMVSHEGACKIW
                     HTFRVKQK"
     gene            13462..>14368
                     /locus_tag="PKM87_07595"
     CDS             13462..>14368
                     /locus_tag="PKM87_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNQ29967.1"
                     /translation="MNGNLLAVIVSITGLVIALAMYGIVGWFRKKAEQTESQLDDILL
                     AAFGTPAVVAVLVITFFLSLQVADLPAQFQFILDSKYVNVIWILLGAWIISTFIYTMI
                     ETYGHRIAAGTKTDLDDRMIALAQVASKYVIWFAAFLLVLYVLEIDITPFLAGAGIAG
                     IALALAAQDFLSNFFGGAIIAVDKPFKINDRIMIDQYVGDVICVGARSTRIKTLDNQI
                     VTFPNKKIMDSYVINYAQPDMRMKVRVNVGVAYGSDIDKVREILMEIAKDASRHYPFI
                     TEDPDPSVYFLEFGASSLNFQLVVWT"
BASE COUNT         2967 a         4144 c         4048 g         3209 t
ORIGIN      
        1 ggaaaaacag tcgaggaagt tgtcctgaaa gggaccctgg tcactgccgg ccggggaaga
       61 atgatcattg gcgcagtcgg ggatgcaaca caaatgggcc ggatcgccgc ggatctcgct
      121 gaggggtccc gccccgagac cccgctgcag ctgaaactcc gccgtctcgc aggactggtc
      181 agcaggttcg ggtacctgat ggcggggctg atcatcgcaa ccctgatgat cgaggggatt
      241 ttcaccggtg ttttcacagg gagcccggct gcaattgccc actacgtact ggacagcttc
      301 atgttcgcgg tgattattat tgtcgtctcg gttcccgagg gccttcctgt cagcgtcacg
      361 gtctccctgg cgctcacgat gcggaagatg acccgggcgt tcagcctcgt ccgccgactc
      421 atcgcgtgcg agaccgtcgg ctcggtcacg gtcatctgca ccgacaagac cggcaccctg
      481 acgatgaacc agatggacgt cgtggcgtca tcggttgaat ttccggatct ccagcccgga
      541 gtccccacca caccgaacgg ctgggtcacc ctgaacgccg cagtgaacag caccgccgat
      601 ctcgaatccc gggaagggag gctcgtgaca gtgggaaatt caaccgaagc agccctcctc
      661 cgctggctcc accgggccgg gctctcctac caggagatca gggcccttca tcccccagtc
      721 acccaggagc tttttgattc aaacaagaag cagatgtcga cgatagtctt ccttgagaat
      781 acattgttca tcctcgtcaa gggtgccccc gagattgtcg caacgcagtg caatccctcc
      841 ccagacctct ctcatatcca ggcgctcgct ggccgggcga tgcgaaccct cgcgtttgcc
      901 cacgctatgt tgcccgccga gggctccgat cctcctccct tgacctggga cgggtacgtg
      961 ggtatccgcg atgaagtgcg tccggacgtc cctgatgcgg tccggacctg caacgaggcg
     1021 ggcatcacgg tgaagatggt gacaggggat agtcccgaga ccgccgcagc aatcgcacgg
     1081 gagacaggaa tcctaaccac cgggacagtc atgctcggga gcgagttccg gtccctgccc
     1141 gatggagcac gacccgaagc cgcccggcgc ctggaggtaa tggcacggtc ggagccttcc
     1201 gataagctgc tcctggtcca ggccttacag gggaacggcg aagtggtcgc ggtcaccggg
     1261 gacgggacga acgatgcccc cgcgcttcgc aatgccgatg tcgggctcgc catgggaatt
     1321 gccgggaccg aggtcgcgcg ggaggcaagc gatatcatcc tgctcgacga ttccttcccg
     1381 accatcaaga acgccgtatg gtgggggagg gcgctcttcg agaacatcca gcggtttctg
     1441 atcttccagc tgaccatcaa catctcggcg gcgatgctct cgttccttgc ccctgtcttg
     1501 ggataccctc ccccattcac catcatccag ctgctctgga tcaatattgt catggattcg
     1561 ctcgcagcgc ttgccctgtg ttcggaggcc ccgcatccag ccctgatgca ccgtaaaccc
     1621 atccctcggg acgcacatgt gattaccccc tacatgctca gggcgatcct catcaccgca
     1681 gcggtgtatg tcctggtcgg gatgctggct gtaatttttg gcctcccgtt catggagact
     1741 cccgaacagc aggagaccgc ctttttcgca gcctttgtcg tggcacaggt ctggaacggg
     1801 atcaattgcc gcggtatcaa cggcgtcatg ccgccgttct tccggggaaa cccggcattt
     1861 ttcgggatca tggcagggat cgtggtgatc caggtcctta tcgtccagtt cggcggagcc
     1921 ctgttctcga cggtcccgct caccgcattc cagtgggtgt tcatcatcct ctgcacctcc
     1981 ccggttctcc tcctctggcc ggtgatacgg ctgagcgaac ggtacatccg gatatcccgg
     2041 agatcaggga tttagagtca gggcggatta catcgctcaa cccctaaaat tatttacacc
     2101 aactccttcc agggtctcat catcacatgg acagcagcct aataatcacg aactcataac
     2161 atactgggaa tgatctcccc ggacctactc tcgctgtccg ctattctcct caatattgag
     2221 aaggttatcc cccagttgat ggagacctac gggatcctga tctatgcaat cctctttgcg
     2281 atcatattct gcgagactgg ccttgtgttc gccccttttc tccccggcga ctccctgctc
     2341 tttctcgtcg ggttcttcgc cgcgggcggc ggtttgaatg tatggattct cctcgttctc
     2401 atcaccattg ccgcaatcgc aggagattca ttgaattatt ggatcgggaa ctatttcggc
     2461 gacaggctga tccacgggaa gagatgcctg gtcgaccgcc gccatgtcga ggtcacccgg
     2521 cagtactacc ataaatacgg gaagaagacc atcatcctcg cccgatttgt tccttacatc
     2581 cgatcgtttg caccgtttat tgccgggatt gtgaagatga attacccggc attcctgtcc
     2641 aataatatca tcggcggtat cctctgggtc acagggttca tatccctggg ttacctcgtc
     2701 gcatcccttc cgctcttttc ggagaaccag gacctgttcc tctgggccat cctgatcatc
     2761 agcatcggga tatgcctcct gatgatgtac aatttcagga agacgatcaa ggaatgcgag
     2821 attccttccg acgtattgat cgaggatctc tcaagggaac tcaggcagga ttgacccgtg
     2881 cggggactca ctttctccgg ctgcccgggt ggctcaggct ctttgggatg taaacgggct
     2941 gcagcctttt ttcgcatata aagcccagga gcaccataga tttctcgtca ctgcatcaca
     3001 gcagcaagag aaatcattgc cagcgaaaaa aagtggatat cggaaccttt ttcactcctc
     3061 ttccaggggg aactctttgg tctccctggc caggaagtac ccgagcacag tcctgatgat
     3121 caccaccacg gcaaggagga tgagctcttc ctgcgtgggg gcgatgaagg tagtgagaat
     3181 atctgcggca atgaagaatt cgaggccaaa gacgatcttg ctggtgaaat ctctgcggat
     3241 gtgattataa gagatgtccc tcttcatcac ttccctgtag attaccctga tgaccgctct
     3301 caaccctcca tatattatca gtgctgcacc ggctatgccg aatatgaagg caaagatctc
     3361 gatgatatca gtggataatg caaaatccat caggggttca cctttgagtt tcaggcacgc
     3421 acgctgccat actacacact ctatcgaacg cggtggagaa ataagtggcg gataccatgt
     3481 tgaccggact attcactcag aacctgtcgg gtgggcgttg aattgataca caggagtgag
     3541 ggacgcatgc gtttccacca tggtcttctg aatccaaacg atgacctcaa acctgcaggt
     3601 atgcgaactc ataaatccca acgtctcttt ctaaaggata atcggcaaat ctgtcatcca
     3661 gcccagatta tccccccatt tttttgtatg cccggctgcg tgcctgtcca ttgaagtgat
     3721 tctctctcaa taaaatacca gatgagccct ttacagagac accagcacag gagatgtctg
     3781 ggccattttt tttatacccg cagccgatac tcgctgtgcg gtcgagcaaa atgagcgata
     3841 acgacaagtg tgatgttcat ttgaaaatgg gatggaaagg gggtgcgccc gtaatattcg
     3901 ggaagcgttc ctcaccaccg gtcgggaccg gtccattccg gtctgttcga attcgagcgg
     3961 gcatcccgcc gacctctgcg ggccatgccg gcataggggc ggcgtggatc aggatggtgc
     4021 ctgtggtgac atgatctcat ctgataggcc tcctgcagcg tgtgggactt tggattcctg
     4081 cgttttccag gtgtgaggga taagtgttta cgcctgtgtg tcactggctt ttggcaaggg
     4141 atctcaattc ctgaatgcca cattgccctt ccgttttaag gcattgcaca atgcgtgcgt
     4201 tccctgttgg aaccactgcg tgcgattact gatcctccgg gttaaaaaag agcggtctct
     4261 ttttccctaa aaaaaagcca tttcactcca tgatgtccga caatttcact cctatctcaa
     4321 tttccatcag ggaatagggg aagaatattc atctttatac ctttagggac cgttaatgtg
     4381 atctgaaaca cgattttgca tggtgccgct cgatctcttt ctcctgtgca tgctgggcgt
     4441 cctgattggc gccatctccg cactcctggg agtgggtggg gggttctttt acgtgccggc
     4501 cctcgtgttg ctgttcggga ttgatccccg catcgcggtg gggacgagcc ttgccattat
     4561 cacgtgcaca accctctcgg cgtcaatagg gtacggtctc cagggtcagg tgctgatgaa
     4621 gagtgccgcc tgccttgccc ttcccgggat ggcctttgct gcgttcgggg cgctcatcac
     4681 cgcgttcctt ccctcgcagt acctggtcgc ccttttctgc gtggtccttc tcttcatggg
     4741 catcaagatg atctacagga agttccccct cgtctttccc ctgctcgttg gcccgtgctg
     4801 gaaggagcgt ttcggcaaac tccatgaaaa gggggaggaa ctatttgtat accctgttca
     4861 cctggtgaca tggggttccc tcgccggatt catgagcggg ctgaccggaa tcgggggagg
     4921 catcgtgaac gtgcctgctc tcacgattgg cggtattccc attcatttcg cagtggcaac
     4981 ctcggcagtc gtgatcctca tcacttcgct ctctgcgacc gggacgcacc tcgcattagg
     5041 aaatctctcc attccctttt tcgttgcaat tgctgccggt gcagtggcag gagcccaggt
     5101 gggagtccgc ctctcgccaa aggccccgcc gaggatgctc gaactggcgg tggggacgtt
     5161 gctgatcgtg gtggtggccg ccctcgtcgt caacacgcat ctcatatgag gccggcagcg
     5221 tctgttcttg atggataaca ggagggccaa ttttcgaaat ggatctcgaa aaaaagcgat
     5281 cgatcaatag aaattcctga aaacagatac gtttcacaaa gagatgcgct ccattgaacc
     5341 gcaccaggcc gccattgccg ttgaccttgg tgccacgaac atacgggcgg gccttgttcg
     5401 tgaagacggg cagatcctct cgctcctcat tgccccgaca cccccgcacc ccgaagatgc
     5461 agatgtcatt ctcaggagcc tcgagagcct catcgcccga gccgtgtccg caactgggat
     5521 gcccccggtt gccgggatgg ggatctctac ggcgggacct gtcgaccttc agaaaggcgc
     5581 ggtggtcaac ccgccgaaca ttcccctccc gttcatcccg ctggccgagc ccctcgggga
     5641 acggttcggc cttcctgtcc ggttcatgaa cgactgtcat gcaggtgcgc ggggggaggc
     5701 ggcgtttggt tgcgggcgag gcgtcgaaaa tttcgtctac ctgacgatct ccaccgggat
     5761 cggtgcaggg gtctacgagc agggaaggct gctcttcggc gaggacggaa actttgcgga
     5821 ggtcggacac ttcgtggtcg agaccacgtg gaaccttccc tgcgggtgtg gagggaccgg
     5881 gcactgggaa ggatgcggtt cagggaggtt catccccgcg ttcttccttg agtggtgccg
     5941 atcgtcgaat catcggtctc ctttccggaa cctttccgat gcagcggcca tttttgcggc
     6001 tgcagcaggc ggagacccgg tcgcagggga gtttgtctcg gagcttggaa aggtggaggc
     6061 gagagggatc tcgctcctca ttgcggccta tgaccccagt cttattgtca tcgacgggag
     6121 cgtggcaagg aataactggc atatctttga aacatctgtc ctcccccatg tccgctccgt
     6181 atcaggcaag atcccgcaga tgcgcctcag cccactcggc gggaacgcac cgctgcttgg
     6241 cgcggcatgg agcgtgttcg accggacgcc tgtcccctga tggacgggaa cctgacttcc
     6301 ggttcgttct cttctctccc ggaaggtgaa tcacaaccag cggagaattc ctgtccggaa
     6361 tcggtcgggt gccatgaacc tcccggttca actcccgttc cttccgattc aagggagggc
     6421 ccccgcgttc ccgatcctga cccgggaaca gcagcacatt ctattatcgt ccctcgcgtg
     6481 agggatgaga ctgaaaaatg atgaacccga ttgcacgcag ggtgcgcggg cctctcctga
     6541 cctgcatctt cgtctgcatg gtgttccttg gtgccatcac ggctgcgtac acagggatat
     6601 gaagcgaggg gatgtgcctg cattacgcac aaccagaagg agggacgttc tactcctgga
     6661 atagaggagg ggttcgggaa tcctccttca ctacttccat ataccacatg gaaaggacac
     6721 cccggataat ctccttcagc cgttccatgc gctgcatgtc aagaagggcg ccctgctgtg
     6781 ggctcgaatt gccaccctca tacagtgagc ggttcacctc gacctggatc caggggatac
     6841 ccttgtgcca gtagtgcgca ttcgagatga accctcccga gaacgggtgg ttgagcgtga
     6901 cgctccctgc tcccggaaac tcgtcccgga acctttctgc cagctgccgg atccactccg
     6961 gcgggcaggt acagagtgcc cctcttctcg ggtgtccgtt ccggtccccg ttgttaccaa
     7021 ggcatatgag tggccgcggc tgtccctcgt caggctggcc tggcaatccc acagggacca
     7081 tgctgtggca gtcgaacgcg actgcgatct tccggctgtc cagcagactg tcaagttttg
     7141 catggtaagg gaagtagtga cgcaggagga gcctgtgaat tgcctggata tctggctgca
     7201 tgccttcttt ccagaccggc gccccaagcg aggtctggaa cttgaccacc ccgtcacggc
     7261 gccggggagg aagatggtac ggagggcggt tcaggtcgac gataacccgc gacaccctgg
     7321 cgctggcaaa ggctttcacc tctgccttga acccaaaaag acgttccgat gcggggtcgc
     7381 tgtaatagga gagtgcaggc ggatcaaggg cgactgctcc cgcgacctcc tccgggatgc
     7441 cgactccccc gtggggaatc gagatgagaa tcgggagccc tggtgacatt cactgccccc
     7501 ccttgcatgg tactacccgg ttgcggctcc cgtcaaggta gtgcgggtaa aagtcatcgg
     7561 cctggatgcc atagaagtgc acatcgtgaa acgaccccca cttgcatgcg tgccctttca
     7621 ttgcaccctc aaagtgcata ccgatgcgca cgagcacgcg ggatgaggga gtgttccagt
     7681 cgaggtgcct cgcgtagatg cggtgtaggc cgaggccgag gaagccaaac tcaagcagcg
     7741 ctcccgcggc ttcagtcgcg tatcccctgc accggaacgg ccttcctatc cagtacccga
     7801 gttctgcatg ggaatgtgca cagttcaccc tgaggcttgc agcaccggca atgtcggtgg
     7861 tctccttctc ggttatggca aagatgtagt gttccccacg ctgccagagg ccctggtgct
     7921 gccctatcca ctgctccgca tcctccaggg tgtagggatg ggggatctca agcacgcctg
     7981 ccgcaatgca catgtcatcg gcatgttcct gaacggactg ggcatcgtct atcgagaaac
     8041 gtcggagcca taacctcggc gtattcagga tctctcgggg gggcatggaa cattcacatg
     8101 tctatttgga accgggtatc atatgagact gtagttcctg tgatacccgg aaaaaattat
     8161 ccgcggggtc acacgccagg gcgggccttc ttcacgagca gggccacatt ttaagatgga
     8221 cgtcgccaga actgtgtaag agagagagca gatgaatacc tccatccttg cccggaaggc
     8281 cgtggtgatc gcctcccttg gagcagcagc catcatcagc gggtacttcg gcctcgccct
     8341 gtctctcggg accgccacgg tcatggacac gcgtgacatt cttgtcgtgt gtgccggggt
     8401 gcttgccggc cctgcaggag gcgcactggt ggggctcctt gcaggtctcc ctggacctga
     8461 tcccctctca gaagtatcac tctacatggt tggcggcctc gccgtcgggt tcattgcaag
     8521 gtattgtgcg gccaatcggg aatggatgcc cactgctgcc cttggactgg gattcgggta
     8581 cgtgctttca ggggcaatcc tgatgttcac ttcgtggtac gacgcggttg cggcgcttgc
     8641 gttcaagtcc cttatcatgc tgaacatctg tatcctcgtc ctctccatca tcgactccct
     8701 ggatcaccgg atcttctcat gggacaggcg gtctccggcc cctgactctt ccggctcgcg
     8761 gtgaggcggg gcattctctc ttgcctgacc attccccccg tgtggaccct ggctcatttc
     8821 cataaagaca gagcaaggca gggtaacccc cggatctgcc cattatgaag ggcagaaggg
     8881 gatgttgcac atccattcat tccatgttga tcgaggaatg ctgggacaag aggttcactg
     8941 taaggctcgt tttctgttgg ggtatagaca gagaaaaggg cagttcacat gaagagccaa
     9001 aggtcatcca ggcatccggc cgcgaggtac ccgatgcggc caagttgggg agttgatacc
     9061 ggatgaccag gccattcaag gtgtggaggg ctgcagaccg tccacatgga gggtgccaga
     9121 caacaaatca gagatcatgg aatcatatgc atgatcactg tccagcatgg cagatatggt
     9181 ttccactgac gataaagatt atgtcatggc ttgatgaacc agtggatacg gggatccaaa
     9241 atctcccata tcttgttcat tctctaaaaa tatccaatat tatggttcaa attattcacc
     9301 cggggcgtat tcacaggatt attgttttat ggttgagagg cgctctttga atcagtgaca
     9361 gggtcaggca atcagtgtgc atataagcaa acgatggctt gacatatccc atgcacccca
     9421 tatcacatgg aaactaatga cgaacgacgt caaaaagacc tgcgatgtat gcgggaggaa
     9481 agccatcggc atccaggtac tgggatgctg cgggtcggta gtctgtgaag tgcatgcaga
     9541 ggaccggctc aaacacatga gcccaggtga gaagatcgag tggggttcat gcttctttta
     9601 ccggtacgag tgacatatag gtttaaaaaa agcgcttcgt atatgggggt tcagggatcc
     9661 ttcactatca ataggggttt tgtcttttac tgatgaatgt cacgcacgca atcttttctt
     9721 gctctgcata tatatagggg gggaactatg cagtttttaa aaaatcagcg attttacact
     9781 ctgcccgtcg cgaaagaccg tcaggcggcc ccctgtctct gagacaacaa ttcccaccgc
     9841 cccggtcacc acagtcattg ccgcaacaga gcggtggcgg gtgccgtacc cggcaggaag
     9901 cgcgaggggg acgtcacccg gaaggaactg ccgcccggac gccacgatga acccgtctcc
     9961 cccgatgatg aacccgccgt cgagcatcgc aaaattcttg atgcaggtct tgttcccggg
    10021 gtttccgaca tacctcattt cgttctccat tgaattgaac gggttgagga taagcggctg
    10081 ggatttggcc atgaccgagg aggcgtcgcc aaccaggaac gatgtcccga cccgttttcc
    10141 ctcccagccc tcccgcgaga tctcgacgca tatggagaag acatcgtcaa agacatcccg
    10201 cttgatatcg gtccctttga ggaggcgtgt cctccagggg tcggtgcgga tgccgcacga
    10261 gtcagggcgg gaataatcgg tgatgtcttc gcctacgcag acgatgtccg ggacgacccc
    10321 gtctctctca acccccacca tcttccacgc gatgcaggcc ctgcgcaagc cggggagcgc
    10381 atgttccacc gcgatgaccg aagattcatc tgcgttcctc gaacatccgg aaagcattgt
    10441 catgaagtcg cgcgccgatt gcgaaccggg tggaaccagc gtgccgatga cattgcttcc
    10501 tgcaacatca accgaccgga acccaaacat catctcggct gcgggattgc agtacacaat
    10561 attcccatca gagtccgtcc ggatgacaat tgcccccatg ctctcgaaaa ccgcgcgata
    10621 cctgctctcg atctccatga ccttctggtc aaaagaccgc tgcagttcga gaagcgactg
    10681 gtggcagcgg atgtcctcgc agatgcagag atacatgtcc tccccctcct tcccggcaag
    10741 ctttcgcatt ttcagcctga tggggacatg agcaccatcc ctcttcctga gcactacctc
    10801 tctctgaacc cagcccgcac cgcccttcga ctgccttccc agctccccaa accacctcat
    10861 gtcctcttcg cgggggagga tggaatggaa cggtctcccc gccatcgtgg agggagagta
    10921 tcccaggata tgcgccatct cttcgttcat ccaggagata tcgggatgtg tcaggataga
    10981 gagagggaca ggagccgaag agatgacgtc gtaaggatgc ccgaacgacg cttcaaaatc
    11041 gcgcctggca ttcctgcctt tcatcaattg agtgaggttc ctgcccaggt cagagaagag
    11101 tgccgggagg ctggaagacc cctggagcgg gaactccatc ctctggtgcg agtcctccac
    11161 cacgatagta tcccctgcct ggagggtgaa ctgggacgaa ccacgccgtt tccgttcgtt
    11221 cttcagtctt acgaggggga tgtcgcggga tgacgcaacg gcctccagcc tcgagccgtc
    11281 ccgactggaa tcattggaga tgaccaggga gtaagagcgg gcgaagagaa gttcgatacc
    11341 ctcccttgaa gaagggacac gctcgacggc atatcctttc tgcgtggaga gatagtctcc
    11401 gaacagcgag atgaggtcgg tccggtcatc gacgagcagt atgtgaacct tgtcaggcgg
    11461 catctctctt ccctatcccg gggtatgtgt gtgactggaa gggcagacgg ataaaagtct
    11521 tcctggcatt cagatatggc gttccccgac cctctccggt gggatgccat ggagacgccc
    11581 ataccatgtc acgaagatct cctggaagta ccatgctgca atctcgaagt cccctcctcc
    11641 atagataacg ccaccatcca tcggaaagag aatgatcgta agggaagaac cccttgcgag
    11701 aggggaagca ggatcaccgg tgatcgccac gcagcgtgcg cctgcgcccc gtgcgctctc
    11761 gagggagcag atcacctctt tccggtcgcc ggacttcgag aaaaggatga caacatcatc
    11821 ctgccgtatg gggtcccgga cctggtcccc gagccagaag acctgttgtt ccggggcaag
    11881 gaagtgccga agccgtattg ccattgcaag acccgcagtc ccgctcctgc cgaatccata
    11941 gagatggatg gcaggcgagg actcgataag gcctgcaagg gccctcgatt gccgttcgcc
    12001 ttcgaccagg gtgccggcaa gccgctttaa atatgcactg tactcatcgg caattcttga
    12061 catatcctgc attgaatgat cccctttaca ccattgaact ggtattgggc ttatgagaag
    12121 taatggattt cggaagggat gaatgtgaag gggacgcccc agccgggatt cgaacccggg
    12181 tcggaagctc cggaggctcc caggatatcc actaccctac cggggcgcgt gagagaagat
    12241 accgcaacgt tacggaattg ctgcggttca taattatggc gcgtcttatc gtaaatacat
    12301 ttgtcatcgg caatcggaag aatgaatgga acgcccggtt ctgacaatgc accgggctgc
    12361 gcctactttt gcttgacacg gaacgtgtgc cagatcttgc aggccccttc gtggctgacc
    12421 atgcatggcc cgatcggcgt ccgtggcgtg caagattttc caaagagcct gcagtcggtg
    12481 ggttctgcga ttccccggag gactttatcg cagatgcagg ccgagtgctt ctcgacgtgg
    12541 cggaaggtga ggtcaaactt cttcttggcg tcgtactgtt cgaactctgg cctgagtccg
    12601 agtcctgagc gcgggatgac cggaaagccc cgccactcag catcaacggg ggtaaacacc
    12661 tcgtacatca gctgcctggc ccgggtattt ccttcccgtg tcacagcccg ggggtacgcg
    12721 ttctcgactt tggcctctcc ggccctcacc tgttcgacga gcatcagaag gccgagcagg
    12781 atgtcctcgg gttcgaaacc tgccaccacc tgggggacgg ggaactgctc gtagtcctgg
    12841 tagccgacga ccgtgcagac atggcccggg aggaggaacc cgtggagttt tgcctcgccc
    12901 tgctcgagga gccatttcat tgcagggggg accaggcggt ggcacgagag gatgctgaag
    12961 ttctccggag gccgggtgag gagggttgcc gcaacggtcg gggcagtggt ctcgaacccg
    13021 accgagatga atgccacctc tcttgtggtg ttcctcgcaa tctccacggc cttgtgcact
    13081 ccctgcacca ccctgacatc gccgccgcaa gactcgagag aacccctgct tccggggact
    13141 cgcaggaggt ctccgtaggt ggcaacgatg caccctttcc cgacaaggtc gagcgcagca
    13201 tcaatctccc cctggggagt gatacaaaca gggcagccgg gccccatcac gatcttgagg
    13261 cctggaggca gcatgctcct cagtcctgac cgggagatgg cagcctcgtg ggttccgcag
    13321 atgtgcatga aagagagatc cctgtcggcg cactcccgca atttttccga tatttccttg
    13381 ccaagggcca tatattggac caattatttt gtatccacga gtgataatgg tactgatatg
    13441 gcgactggga caatattcgg aatgaacgga aatctcctcg ccgtgattgt gagcatcacc
    13501 gggctggtca ttgcgctggc catgtatggg atcgtcgggt ggttccggaa aaaggccgag
    13561 cagaccgagt cccagctgga cgacatcctg ctcgcggcgt tcggaacccc cgcggttgtc
    13621 gcggtgctgg tgatcacgtt cttcctctcg cttcaggtcg cggaccttcc tgcccagttt
    13681 cagttcatcc tggacagcaa atacgtgaac gtaatttgga tccttctcgg ggcatggata
    13741 atatcgacgt tcatctatac aatgatcgag acatacggtc accgtatcgc ggccgggacc
    13801 aagaccgacc tcgatgaccg gatgatcgcg ctcgcgcagg tcgcgtcgaa atacgtgata
    13861 tggttcgcag cattcctcct tgttctctat gtcctcgaga tcgatatcac gcctttcctt
    13921 gcgggtgcag gtattgccgg gatcgccctt gccctcgcag cccaggactt tctctccaac
    13981 ttcttcggcg gcgcaatcat cgcggtagac aagcccttca agatcaacga ccggatcatg
    14041 atcgaccagt atgtcggtga tgtgatctgt gtcggtgccc gcagcacgcg gataaaaacc
    14101 ctcgacaacc agatcgtgac gtttccgaac aagaaaatca tggacagtta cgtcatcaac
    14161 tatgcccagc ccgacatgcg tatgaaggtc cgggtcaatg tcggggttgc ctatgggtca
    14221 gatattgata aagtcaggga gatcctgatg gagattgcaa aggacgcttc ccggcattac
    14281 ccgttcatca cggaggaccc ggacccgtca gtttatttcc tcgagttcgg ggcatcgagc
    14341 ctgaacttcc agctcgtggt atggacaa
//
LOCUS       DAOQYR010000056        14147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_4775_length_14147_cov_8.829052, whole genome shotgun sequence.
ACCESSION   DAOQYR010000056 DAOQYR010000000
VERSION     DAOQYR010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 14147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14147
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4775_length_14147_cov_8.829052"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..775)
                     /locus_tag="PKM87_07600"
     CDS             complement(215..775)
                     /locus_tag="PKM87_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106536.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNQ29968.1"
                     /translation="MILLIDPGFRADSLPRDEFVSPVARIAERSGLEWKYRHYAAMDD
                     AIPTDMEGVIICGTALKDNGFLACPDSFDWLHRTEIPVLGICAGMQVLCLTFGGSVKE
                     GCEIGMTDVHVTAPHFLLPGVSSFQAYELHSHSCEPPPGWEILAVSDRCIQAIRDPLH
                     PRFGLMFHPEVRNDRVVEVFLELCRA"
     gene            complement(772..1452)
                     /locus_tag="PKM87_07605"
     CDS             complement(772..1452)
                     /locus_tag="PKM87_07605"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HNQ29969.1"
                     /translation="MRLGVLCSGGKDSLFACYRAMQEEKVACLVTLFPENPESYMFHT
                     PNLHLVPLQAEAAGLPLVSQKSRGVEDEELGDLSRALSVASDRHGIEGVVTGALMSVY
                     QSSRIQQICSDLDLWCFNPLWYADPDIYMEQLFSEGFRVVVSGVFSAPFDASWLGRKL
                     DRAALSELRGYVRDYRITLTGEGGEYETLVLDAPFFQKRIVIEDAKTTYQNYRGILDV
                     KKAHLEER"
     gene            1685..3064
                     /locus_tag="PKM87_07610"
     CDS             1685..3064
                     /locus_tag="PKM87_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ29970.1"
                     /translation="MQRAILLIAVISSFITPFDGSAVNIALPTIGEEFSLDAVTLSWI
                     TTAYLLSIAILLVPFGKLADMKGRKRIFLVGITIFTAASFLVAFSADAGTLIGLRLLQ
                     GTGCAMIFGTSLAILSAAYPPKERGKALGITIAAVYVGLSAGPFLGGLMTETLGWRSI
                     FLVNVPLGILCILFTVWKLPLEWKNDSGEHFDLAGSFLWAIALIFVMYGLSILPGLPG
                     IGLIFSGIVALVAFGLLELRVPAPVLNMKLFLENRVFAFSNFAALINYSATYAVTFLL
                     SLYLQIAKDFSAEYAGLILVAQPAMQALFSPMAGKLSDRLDPGTVASFGMAVTVVGLF
                     FLTFLDSDSTLPFILGCLLFLGIGFGIFSSPNTNAIFGSVNRDTYGVASGMVGTMRLL
                     GQMLSMGLAMTIFAIMIGNDLQIDENVPLFIESIHVAFLLFFLLCIFGLAFSLVRRKR
                     PGFLQGNNR"
     gene            complement(3116..3634)
                     /locus_tag="PKM87_07615"
     CDS             complement(3116..3634)
                     /locus_tag="PKM87_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29971.1"
                     /translation="MTERDPLPPELKWQIVSRLVTSLPVMYDITFRDAVGDSYDAREQ
                     QIWMHLAREARALAGSANLPTADARDLMETLRVIFGVFFGPDLRTEEVFINADRAVLM
                     IKRCPFIAREMEMRAGPEHLLSRCLSFSIATIEALNPAYTLRFVRSMCTGDRTCELKI
                     MKREDAEKDDTR"
     gene            complement(3658..4758)
                     /locus_tag="PKM87_07620"
     CDS             complement(3658..4758)
                     /locus_tag="PKM87_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HNQ29972.1"
                     /translation="MGEVDNFYVRMKMEVISMGRFAGWEHVEIVDDRGVSRDAIAPVI
                     LSASRATDIPAFYGDWFMHRLKKGHVAWINPWNGRQNYVSFARTRMIAFWSKNPRPIM
                     EHIDSIAEMGYSFYFLFTLNDYVREGIEPGVPKLSERVRTFRDLSDRLGPGRVVWRFD
                     PLLLSDTLSFDDLLSRVERIGSCLNRHCRRMVISFIDIGKYPQVRRSLDRNGFFGVRE
                     FSEREITQFCSGLAEMNDKWNLSISACGEARDLTEYGIRRGQCISGELMREEFAHDTA
                     LMRFLGSPAQGKVMHPGDPPFRHLKDPGQRGACGCIASKDIGQYSTCTHFCRYCYANA
                     SSRRVEENVRRYRASVESGIYHESITGDQQGI"
     gene            4866..6116
                     /locus_tag="PKM87_07625"
     CDS             4866..6116
                     /locus_tag="PKM87_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HNQ29973.1"
                     /translation="MGIRFFQGQKIKYEHEFSQLREIVEILRKEYPREPVYLLTGVLV
                     ANGQIDCVILTKNGPLILDLKSFRGEITGVENGNWVAKTKDGPIPLPNLFLQAKIHRQ
                     DFIDRLIPVCREHFPHIGESNLRKTGSWLYFCRGSTYPDGQIDFRKVKWFRIVTADDL
                     TQKMKFMDSGYTLRIQDMDAIVRGLGLEEYSFEDGAARVPAARKRKSPLISRTTLIIL
                     LIIFGLFTAGIFVVVTFPGAKVAAANALQGVISIVGGWMHTVMQETTKKSTTQEDSQQ
                     AIIYLNRFRIAEGALPIAFDSRAYSIALNRAEDMVEYKYLDYHNPTTGSSALALKHLH
                     GFAENETLIETLYGQWTGYTPGIERQAIDAWMTDPGNRQRLFFAQVSGAIACVDGYCS
                     YIGVRPALVVENATANITGNLTEG"
     gene            complement(6126..7331)
                     /locus_tag="PKM87_07630"
     CDS             complement(6126..7331)
                     /locus_tag="PKM87_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA sulfurtransferase"
                     /protein_id="HNQ29974.1"
                     /translation="MAFQANSRVERVVMLRYGEIFLKSEPVKRHFVGTLLRNCTRALG
                     AAGLSHRAEVHRGRVILHGECPEEIASIVSRLFGVVDVSICTLTLPDPRSVTDAAVAL
                     ASINLRPGLRFAVRARRQGVEGITSQELGARVGAAILDAVPSARVDLGSPEYEVFAEL
                     RDFGGLVYDSRIPAPGGLPWGTQGKILALLSPGIDSPVAMWMMMRRGCEVDALHIHGG
                     GYAGDDVLSTTLRHVSTLSSWCTGFPITLHVADAGDFYDDLVRRAEPRLRCVLCKRFM
                     MRLGSRLVTRGGYLALCTGDSIGQVASQTMANLSVISQAATVPLVRPLVAFDKQDAVN
                     LAKKIGTFERQPGDLACSAVPRVPATTSRLEGVIAMEEKIGMDDHVSYAMERLRLYCA
                     LNGALVKPE"
     gene            7622..8485
                     /locus_tag="PKM87_07635"
     CDS             7622..8485
                     /locus_tag="PKM87_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNQ29975.1"
                     /translation="MYEHVLVPFDFSPDSRHTVECLTQFPGVSRVVLLHIVYTRYPAQ
                     ADGTVDPSIDYARLRLEEYARTIDLGSISIQTRIEGITGGNISPVVNRVAAAEGVSLV
                     AMGRRGMSVIEALLLGSVASDVLRYSDRDLLLINAPASGETNSPGGCIGLLSHLMVCT
                     DFSRPEIVSLSMEDLPFASRVTLLHVVSTGDTREEVNLHSSDAWTRLKELREAYSRLK
                     IPVRATVCEGDTAEEIIRHARENQVSLIVMKSSGKRGFIRNFIGSTTTHVTRNADIPV
                     LVLKRFEAEKK"
     gene            8620..10215
                     /locus_tag="PKM87_07640"
     CDS             8620..10215
                     /locus_tag="PKM87_07640"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HNQ29976.1"
                     /translation="MISWELIFAIIPGIILFLYGIEQFSREFQVAAGEQFRALIQKFT
                     RTPLRGTIAGAIVTAVVQSSTATTVITVGLVNAGIISFTASLGIIIGSNIGTTFTTQL
                     IALNLTAFAPVFILAGFLIGIAGGRYRVFGRPIFYFGLVFFSLSMISTIMEPYRFDPQ
                     LISLLSILDNVFLEIAFGFIVTIIFQSSAVTTGLVIVLTQNGLVTPDMAIPILLGANL
                     GTPTTSLLVAWRMNTAAKRAAMAHFLFNFLGVLIFLPFLGPFTDLVVDLGGSPGQQVA
                     NAHLIFNLTCAGIFLVAIKPFSSLVQRVVPGRDDDIVFLPHFLKKPLPDDSKTAVRMV
                     AQEIGHLLETDEEMLKECNHLLDATDTPFQRVQQLREYAQYLDDQIADAVLDLSRRDL
                     ERGDAAYYAVLLRVSSLGRVLADEAGDLSDILIQIRERGLKIPADSVLIQKDILSSCE
                     RNVGILRDAFPEISDTVDSAMRSVDETLRVEITRHYHQHVIRISGGKNPAGSTVSRML
                     FHIEGMAATIREMRKSARMLQVV"
     gene            10380..11018
                     /locus_tag="PKM87_07645"
     CDS             10380..11018
                     /locus_tag="PKM87_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2150 family protein"
                     /protein_id="HNQ29977.1"
                     /translation="MTMAKKKEKAASAEEPLKLFYIFYNQERWDNWINTLREANFEAS
                     PQSEEMPEGYTTLYNFSMDITLSVLKIVKLFQNGRYTKEEALEKLNAVEVIVMGEVLQ
                     DNLEEYVESLQLSLLVLFAGCRKFLEGEYKTDIKTLVKEGRKTIDSDMEKALDIAADI
                     AASVINGASCCGKYVKDDIEQPTLFDEWLIEAESMAEAVSSLKNFDEEAGET"
     gene            11015..11467
                     /locus_tag="PKM87_07650"
     CDS             11015..11467
                     /locus_tag="PKM87_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5814 domain-containing protein"
                     /protein_id="HNQ29978.1"
                     /translation="MISQKARFRHARKIERLAGYRLPDQAFHGATLDAISSRLDFDRL
                     DHTLHQQILNFIHSFLRCTCRDAPMCGCPEKKFSREILELRENGLDHRQIAQYLQEEY
                     GIDIYPADILSFLEDSVHVLEAIRDIADLQAMPDLCKNADTHIELLER"
     gene            11664..12050
                     /locus_tag="PKM87_07655"
     CDS             11664..12050
                     /locus_tag="PKM87_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29979.1"
                     /translation="MIRSLIVTWTPRPGEPHPCAICSDSGLSFLELLSLVRPLLERDG
                     IPVTLVENLLFPGSQTEENGFLLNGRPLEELLLESDRAQFLCHSSRCQPYVSGVDITR
                     NERGIRCIRAPEILFRKAILRSLEEA"
     gene            complement(12482..13450)
                     /locus_tag="PKM87_07660"
     CDS             complement(12482..13450)
                     /locus_tag="PKM87_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNQ29980.1"
                     /translation="MKQPNPLQAAVFLILFLLGLWVSYSLFILTGQVIFFAIALAALV
                     VALYLAAALQIANQWEKAVVLRLGKYKGLYGPGVFIITPIVETVAYWIDTRIITTSFT
                     AEKTLTKDTVPVDVEAVLFWKVVDPQRAALEVEDYRTAVSWAAQTALREVIGRSNLAE
                     MLEGREKLDLELQRIIEGRAEHWGIHVSSVEIRDVLIPAELQDAMSMQAQAERERQAR
                     VILGDSERQVAEKFEEAARTYQDNPTALHLRAMNMLYEGLKEKATLVIVPASVLDTMT
                     LGDTSGIIALSRQIQDQATVRGQGEKPRNAKEKRQSGKDESEEGAL"
     gene            complement(13554..>14147)
                     /locus_tag="PKM87_07665"
     CDS             complement(13554..>14147)
                     /locus_tag="PKM87_07665"
                     /inference="COORDINATES: protein motif:HMM:NF014527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNQ29981.1"
                     /translation="VADLEVILGIDCTITLHREYGFCYRLDVEVTFFSARMASERRRV
                     WMQVRRGEEVLVPFAGVGPFAIPPASRGALVTAIEKNPLACGFLRENVSLNRVVARVF
                     VYEGDVEDVLPDISAKFDRAIIPAPYGHDQALFSILPMVKKGGTIHFYTFKRASQVPA
                     IQSEYESAGVEVRSIRRCGYVGPGVARYAFDMVKRPR"
BASE COUNT         3100 a         4155 c         3769 g         3123 t
ORIGIN      
        1 agtctggaag ttcaacacct acgggatgac gcaggcacag gcagaatatg ttgcccattc
       61 gtttgtcgag atcgcagccg aatgcggcct tccggtcagc gaataacccc gctcaaccat
      121 ttttcaaagg aaaatcagtt tgagcattca ttttgtaggc atatgtctcg atggatcatg
      181 tggttttttt aaaggcatgg catcctcttt gccatcaggc cctgcacaac tccaggaaca
      241 cctccaccac gcgatcattt cgaacctccg ggtgaaacat caacccaaaa cgagggtgaa
      301 gggggtcacg tattgcctgg atgcaccggt ccgaaaccgc gagtatttcc catcccgggg
      361 gcggttcaca cgagtgtgag tgcagttcgt acgcctggaa ggatgaaacc cccggcagca
      421 ggaaatgcgg ggcggtcacg tggacgtccg tcatcccgat ctcgcacccc tccttcaccg
      481 accctccgaa tgtgaggcag agcacctgca tccctgcgca gataccgagc accgggattt
      541 cggtccggtg gagccagtcg aacgagtcag ggcaggccag gaacccgttg tccttcaatg
      601 ctgtcccgca gatgatcacc ccttccatgt ccgtcggaat cgcatcatcc atcgcggcgt
      661 aatgccggta cttccattcc aacccgctcc tctcggcgat ccgtgcaacg gggctgacaa
      721 actcatcacg tggaagggaa tcagccctga atcccgggtc gatgaggagg atcatcgctc
      781 ctccaggtgt gcctttttca cgtcaaggat tccccggtag ttctggtacg tcgttttcgc
      841 atcctcgatc acgatcctct tctggaagaa cggggcgtcc aggacaaggg tctcgtattc
      901 tcccccctct cccgtcaggg tgatacgata atcacggaca tacccccgaa gctcggagag
      961 tgctgcccta tccagtttgc ggccgagcca cgatgcatca aacggtgcag agaacacccc
     1021 ggatacaacc accctgaagc cttccgagaa caactgttcc atgtaaatat cagggtcagc
     1081 ataccagagg ggattgaagc accagaggtc caggtcactg cagatctgct ggatacgtga
     1141 cgactggtac accgacatca gcgcacctgt taccacaccc tcgattccgt ggcgatcgga
     1201 agcaaccgaa agggcccgcg agagatcgcc aagttcctca tcctcgacac ctctgctctt
     1261 ctgcgagacg agcggcagcc cggcggcttc tgcctgcagc gggaccaggt gaaggttcgg
     1321 agtgtggaac atatagctct cggggttttc cgggaacagg gtgacaaggc atgcgacctt
     1381 ctcctcctgc atggcgcggt aacaggcaaa gagcgagtcc tttccgccag aacagagcac
     1441 cccaagcctc atcggctctc tcatatcacc cgcacattct ggcgggagac cagatgaagt
     1501 tcactgcagc aatagccggg atgcgaagga ccgggtcggg gcacagggac cttttttcgg
     1561 atccagatgt cagattcaca ctcaagaatt ttcatcagct ttcgacacac agatatttac
     1621 cagtctttta aggagagaat gccgtacgga aatgccatgt cctgggaaga atgtcaccac
     1681 ttccatgcag agggccattc tccttatcgc ggtgataagt agtttcatca ccccctttga
     1741 cggctcagca gtcaatattg ccctccccac aataggggaa gagttctcat tggatgccgt
     1801 gaccctctcg tggatcacca ctgcatacct cctctccatt gccattctgc tggtgccgtt
     1861 cgggaaactg gccgacatga aagggaggaa gcggatcttc cttgttggca tcaccatctt
     1921 caccgcggca tcgtttctgg tcgcgttctc agcagatgca ggaacgctga tcgggcttcg
     1981 cctcctacag ggcacgggct gcgcgatgat cttcggcaca agcctcgcaa tcctctccgc
     2041 agcgtatccc cccaaagagc ggggaaaagc acttgggatc actattgccg cggtttacgt
     2101 tggcctctcg gcaggcccgt ttcttggagg tctcatgaca gagacacttg ggtggaggag
     2161 catcttcctt gtcaatgtcc cccttggtat cctctgcatt ctcttcacag tctggaaact
     2221 ccccctggaa tggaagaacg attccgggga gcacttcgac cttgcaggct cgttcctctg
     2281 ggcaatagcc cttatttttg tcatgtatgg cctgtcaata ctcccgggac tgccgggaat
     2341 tgggctgata ttctccggca tagtagccct ggttgccttt ggtctccttg aactccgggt
     2401 ccccgcgcct gtactgaaca tgaagttatt cctcgagaac cgtgtctttg cattctcaaa
     2461 cttcgcggcc ctcatcaact acagcgctac ctatgcggtg accttccttc tctcgctgta
     2521 cctccagatc gcgaaagatt tctcggccga atatgccggg cttatcctcg ttgcccaacc
     2581 cgccatgcag gccctcttct cccccatggc aggaaagctt tctgacaggc tggatccggg
     2641 aaccgtggca tcttttggca tggcagtcac ggtcgtgggc ctgtttttcc tcacctttct
     2701 tgattcagac tcgactcttc catttatcct gggctgcctg ctgttcctcg ggattggttt
     2761 tgggatattc tcgtccccga ataccaacgc catattcggc tcggtgaaca gagacaccta
     2821 cggggtcgca tcaggcatgg tcgggaccat gcggctcctc gggcagatgc tctcgatggg
     2881 tcttgcaatg acaatttttg ccataatgat aggaaatgac ctccagatcg atgagaatgt
     2941 cccgcttttc atcgagagca tccacgtcgc cttcctgctc ttctttcttc tctgtatctt
     3001 tggccttgca ttctctcttg tccggaggaa gcggccaggc tttctccagg gcaacaatcg
     3061 gtgatcggtc cgtcattacg cgtgactggc agcgatctcc ccctgcacgg gtgaatcatc
     3121 ttgtgtcgtc cttctccgcg tcctcgcgct tcatgatctt gagctcgcat gtccggtccc
     3181 cggtgcacat gctgcgtacg aacctgaggg tataggcggg attcaatgct tcgatcgttg
     3241 cgatagagaa cgagaggcag cgcgagagga ggtgttcagg gccggccctc atctccatct
     3301 ccctggcaat gaacgggcag cgcttgatca tgaggaccgc ccggtccgca ttgataaaaa
     3361 cctcctcggt gcggagatcc ggtccgaaga acaccccgaa tatcacccgg agtgtctcca
     3421 tcaagtcccg ggcatcagct gtgggaaggt ttgcactccc tgcaagggcc cttgcctcac
     3481 gtgcgaggtg catccaaatc tgctgctcac gcgcatcgta cgaatctccc accgcatcgc
     3541 ggaaagtgat atcgtacatc accgggaggc tcgtgaccag gcgggacacg atctgccact
     3601 tcagctcggg ggggaggggg tccctttcag tcatatggtg gtgttgccgg gcgtgggtta
     3661 tatgccttgt tggtccccgg tgatcgactc atgatatatc ccgctctcaa cagatgcacg
     3721 atatctccgg acattctctt cgactctgcg cgatgaggca tttgcatagc aataacggca
     3781 gaagtgtgtg caggtcgagt actggccgat gtccttgctc gcgatgcacc cgcaggctcc
     3841 cctctgcccg ggatctttga ggtgccggaa cgggggatcc ccgggatgca ttactttccc
     3901 ctgcgcggga gatccgagga accgcatgag tgcagtatca tgggcaaact cttctctcat
     3961 cagttccccg ctgatgcact gcccccttct gatgccatac tccgtgaggt cgcgggcctc
     4021 cccgcacgcc gaaatggaga ggttccattt gtcgttcatc tctgcaagac cgctgcagaa
     4081 ctgggttatc tcgcgttctg aaaattcccg gacaccaaaa aagccgttcc tgtcaagaga
     4141 cctgcgaacc tgcgggtact ttccgatatc gataaaactg atcaccatgc ggcggcagtg
     4201 acgattcagg caggagccga ttcgttccac ccgggagaga agatcatcga aagagagagt
     4261 gtccgagagg aggagtggat cgaacctcca cacaaccctg ccggggccga ggcgatccga
     4321 gagatcgcgg aatgtcctca cacgttccga cagttttggc acacctggtt caatgccctc
     4381 gcgcacgtaa tcgttcagcg tgaagaggaa gtaaaacgag tagcccatct ccgcgatgct
     4441 atcaatatgc tccatgatag gccggggatt cttggaccag aaggcgatca tgcgggtgcg
     4501 ggcaaaggac acgtaattct gccttccgtt ccagggatta atccaggcca cgtggccttt
     4561 tttgaggcgg tgcatgaacc agtcgccata aaacgcaggg atgtctgttg ccctgcttgc
     4621 agagaggatc acaggcgcaa tggcatcgcg tgaaacaccc ctgtcatcca cgatctcgac
     4681 gtgttcccac cctgcgaacc tgcccatgct tatcacttcc atcttcattc gtacgtaaaa
     4741 attatcaacc tctcccacgc ggccctccat tgtaacatta cgccggacag gcaagctata
     4801 tcaaaaataa gggtaaataa gagtgtctgg atatatattc accacagggc gacaagggac
     4861 gggtaatggg aatccggttt ttccagggac agaagatcaa atacgagcat gagttttcac
     4921 aactccggga gatcgtcgag atcctccgaa aggagtaccc cagggagccc gtgtatctcc
     4981 tcaccggggt gctcgtggca aacggtcaga ttgactgcgt gatcctcacc aagaacggcc
     5041 ctctcatcct cgacctgaaa tcgtttcgtg gggagatcac gggcgtcgag aacgggaact
     5101 gggtggcaaa gacaaaggac gggcccatcc ccctcccgaa cctcttcctc caggccaaga
     5161 tccaccggca ggacttcatc gatcggctga ttcctgtatg cagggagcat tttccccata
     5221 ttggtgagtc gaacctgcga aagacgggct cgtggctcta tttctgccgg gggagcacgt
     5281 acccggacgg gcagatcgat ttccggaaag tgaagtggtt tcgcatcgtc accgccgacg
     5341 atctcaccca gaaaatgaag ttcatggact ccgggtatac cctccggatc caggacatgg
     5401 atgcaatcgt ccggggcctc gggctcgagg agtactcatt cgaggatgga gcagcgcgtg
     5461 ttcctgccgc cagaaaaagg aaaagtccgc tgatatcccg aacaaccctc attatcctgc
     5521 ttatcatatt tggcctcttt accgcgggaa tctttgtcgt ggttacgttt cccggcgcca
     5581 aggtggccgc cgcaaacgcc ctccaggggg tgatctctat cgtcggcggg tggatgcaca
     5641 cggtgatgca ggagaccacg aagaagtcca cgacccagga agacagccag caggcaatca
     5701 tctatctcaa ccgcttccgt atcgcagagg gggcgctccc catcgcattt gacagccggg
     5761 catactcgat tgcgctcaat cgggcagagg acatggtcga atacaagtac ctcgactacc
     5821 acaatcccac aacaggatct tcggctcttg cactgaaaca cctgcacggc tttgccgaga
     5881 acgagaccct gatcgagaca ctctacggcc agtggacagg gtatactccg ggcatcgagc
     5941 ggcaggcaat cgacgcatgg atgactgacc cagggaaccg ccagcgcctc ttcttcgcgc
     6001 aggtctccgg cgcaatcgcc tgtgtcgatg gctactgttc ctatatcggc gtgcgcccgg
     6061 ctctggtcgt cgaaaacgca accgcgaata taaccggaaa cctcactgaa ggataagaac
     6121 ggaaatcatt ccggtttcac gagggcgccg ttgagagcgc aatacaatct gagccgctcc
     6181 attgcataag agacgtggtc atccattcca atcttctcct ccatggcgat gaccccctcg
     6241 agccgtgacg tggtggccgg cacccgcggg actgccgaac acgcgaggtc tccgggctgg
     6301 cgttcgaacg ttccgatctt ctttgcgagg ttcactgcat cctgtttgtc gaaggccaca
     6361 agtggtcgga caagggggac cgttgccgcc tgtgagatca ccgaaaggtt ggccatagtc
     6421 tgtgacgcaa cctgcccaat gctgtcgccg gtgcagagtg caaggtagcc gccacgcgtc
     6481 acaagcctgc tcccaaggcg catcatgaac cgcttgcaca gaacgcagcg gagccttggc
     6541 tctgctctcc gtacaaggtc gtcgtaaaaa tctcctgcat ctgcgacatg aagggtgatg
     6601 ggaaacccgg tacaccacga agagagcgtc gagacgtgac gaagcgtggt ggagaggacg
     6661 tcatcgcctg catatccccc tccgtggata tgcagggcgt ccacctcgca accgcgcctc
     6721 atcatcatcc acatggcaac aggtgaatcg attccaggcg aaagcagcgc gaggatcttc
     6781 ccctgtgttc cccaggggag gcccccgggg gctgggatcc tggaatcgta gaccaggccg
     6841 ccaaaatccc ggagctctgc aaagacttcg tactcggggc ttcccaggtc caccctcgcg
     6901 gagggaactg catcgaggat ggccgccccc acccgggcgc cgagttcctg gctggtgatc
     6961 ccctctaccc cctgacggcg ggcacggacc gcaaagcgca atcctggccg gaggtttatt
     7021 gaggccagtg caaccgccgc gtccgtcact gacctgggat caggaagagt gagggtgcag
     7081 atagacacat ccacgacccc aaagaggcgg gacacaatgg atgcaatctc ttccgggcat
     7141 tcgccatgca ggatgaccct ccccctgtgc acttcggccc tgtgtgagag ccctgcggca
     7201 ccgagcgccc gcgtgcaatt acggaggagt gtccctacaa aatgccgttt tacaggctcg
     7261 ctcttgagaa agatctcacc gtaacggagc atgactacac gttcaacccg ggaattcgcc
     7321 tggaaggcca tctcttcctg tatattgggc gctaaggttc ttaataaggg agatgcgtcc
     7381 ggccgactca tcccagggaa caacgacaag caccgggcaa cattttccga tacgcaatga
     7441 aacacttgcc gggaaaaaac ctgcgcacaa gctcaacgga cgggaaaatg ttaaatttgc
     7501 cgacatcaag gagtaacaaa acgaaaaggc aatggacggg cttttcccgc acggagcgtc
     7561 actctccctg ccagagcccc cttgaaaaga gatcctttcc gacgatgcag gagttttatg
     7621 gatgtatgaa cacgtgctcg ttccatttga tttctctccc gattcgcggc acacagtgga
     7681 atgcctgacc cagtttccgg gagtttcccg ggttgtcctc ctccatatcg tctataccag
     7741 gtatcccgca caggctgatg ggacagtcga tccgtcaatc gattatgccc ggctccggct
     7801 cgaggagtat gcaaggacaa tcgatctggg cagtatatcc atccagacga ggatcgaagg
     7861 gatcacggga ggcaatatct cgccggtggt caacagagtc gctgcggctg agggggtgtc
     7921 gcttgttgcg atggggagga gggggatgag tgtaatcgag gccctcttac ttggcagtgt
     7981 cgcgtcagac gtgctcaggt acagcgaccg cgatctcctc ctcatcaacg caccggcatc
     8041 cggggaaacc aattcccctg gtggctgcat tggcctcctc tcacacctga tggtctgcac
     8101 ggacttttcc cgccctgaga tcgtgtccct ctcgatggag gatcttccct ttgcctcgcg
     8161 cgtaaccctg ctccatgtcg taagcacggg ggacacccgc gaggaagtca accttcactc
     8221 aagcgatgcc tggacaaggc tcaaggaact cagggaagca tattcccgcc tcaaaatccc
     8281 tgtaagggcc acggtatgtg aaggtgatac tgcggaggag atcatacggc atgccaggga
     8341 gaaccaggta tccctgatcg tgatgaagtc ctctgggaag aggggtttta tcaggaactt
     8401 catcggcagc accaccaccc atgtcacacg aaatgccgat atcccggtgc tcgtactgaa
     8461 acgtttcgaa gccgaaaaga agtgagaact catggcccgg cttctgcaca ctgggcaagg
     8521 gcgattcgcg ggaattctct cttcacagag tatcgcagcg attccatccc tatatcgacg
     8581 gcgttctcaa tctcgtgacc ctaccgtgag gatatcccca tgatctcctg ggaactcatc
     8641 ttcgcaatca tccctggcat catcctcttt ttatacggga tagaacaatt ctcgcgtgag
     8701 ttccaggttg ccgcgggtga gcaattccgt gcccttatcc aaaaattcac ccgcactccc
     8761 ctgcgtggaa cgatcgcggg cgcaatcgtg actgcggtgg tccagtcgag cactgcgacc
     8821 acggttatca ctgtagggct cgtgaatgcc gggatcatct cctttactgc atcgctgggg
     8881 atcatcatcg gttcgaatat cgggaccacc ttcaccaccc agctgatcgc cctcaacctg
     8941 accgcgtttg ccccggtctt catcctcgct ggatttctca tcgggatagc cggggggaga
     9001 tacagggtat tcggccgccc aatcttctac ttcgggctgg tcttcttcag cctctcaatg
     9061 atatcgacca ttatggagcc ctaccggttc gacccccagc tgatctcact cctctccatc
     9121 cttgacaatg tctttctcga aattgcattc ggttttattg taaccattat cttccagtcg
     9181 agtgctgtca caaccggcct cgtcattgtg cttacacaga atgggcttgt gacacctgat
     9241 atggcaatcc ctatccttct cggggcaaac ctcggtactc caaccacctc actccttgtc
     9301 gcatggcgga tgaacactgc ggcaaagagg gctgccatgg cccacttcct cttcaacttt
     9361 ctcggggtcc tcatctttct cccattcctc gggccattta cagaccttgt cgtggacctg
     9421 ggggggtcac ccggccagca ggtggcaaac gcccacctta ttttcaacct cacctgcgca
     9481 ggaatattcc tggtcgcgat aaaacccttc tcctctctcg tgcagcgggt agttcccggc
     9541 agggatgacg acatagtctt cctcccccat tttctcaaaa agcctcttcc tgatgattca
     9601 aaaaccgcag tccggatggt cgcgcaggag atcggtcacc tgttggagac agacgaagag
     9661 atgctgaagg agtgcaacca ccttctcgat gcgactgata cccccttcca gcgggtgcag
     9721 cagctcaggg aatatgctca atacctggac gaccagattg ccgatgcagt cctcgacctt
     9781 tcccggcggg atctggagcg tggcgatgca gcgtactatg cagtgcttct cagggtctcg
     9841 agccttggaa gagtcctcgc agatgaagca ggggacttat ctgacatatt gatccagatc
     9901 cgtgagcggg gactgaagat tcccgccgat tccgtcctga tccagaagga catcctctca
     9961 tcatgcgagc gaaatgtcgg aatactccgg gacgcatttc ccgaaatatc cgacacggtc
    10021 gattctgcca tgcggtctgt tgacgagacg ctcagggttg agatcacgcg gcactatcac
    10081 cagcacgtca tccggatctc cggcggaaag aaccctgcag ggagcacggt ctcccggatg
    10141 ctcttccaca tcgaggggat ggccgcgacc atccgcgaga tgaggaaatc ggcacgcatg
    10201 ctccaggtgg tctgaaattt ttcagggcgg gggagatgcg tccggcctcc ccctgcgttc
    10261 tccaggcaga ggattggcct ccactcattt gcccacccga aacctctttt ctttacgatt
    10321 ccaagggagt tattcccgca ccattccgat acacctttaa catacaggag aaaacctgta
    10381 tgacaatggc gaaaaagaag gaaaaagcgg ccagtgccga agagccgctc aaattgttct
    10441 atatcttcta caaccaggag cgttgggaca actggatcaa tacccttcgg gaggcgaact
    10501 tcgaggccag tccacaaagc gaggagatgc ccgaaggata cacgactctc tacaacttct
    10561 cgatggacat cacactctcg gtcctgaaga ttgtcaagct cttccagaac ggaaggtata
    10621 cgaaggagga ggccctcgag aagctgaacg cagtagaagt gatcgtgatg ggggaggtcc
    10681 tgcaggacaa tcttgaggag tatgtcgagt cactgcagct ctcgctcctg gtcctgtttg
    10741 cgggatgcag gaagttcctt gagggcgagt acaagacaga tatcaagacg ctcgtcaagg
    10801 aagggaggaa gaccattgat tcggatatgg aaaaagcgct agatattgca gcggatattg
    10861 ctgcaagcgt gatcaacggt gcatcctgct gcgggaagta tgtcaaggac gacatcgagc
    10921 agcccaccct ctttgatgag tggttgatcg aggcagagag catggccgag gccgtttcat
    10981 cactcaagaa ctttgatgaa gaggccggcg agacgtgata tctcagaagg cgcgtttccg
    11041 gcacgcacgg aaaatcgagc gcctggcggg ttatcggctc cccgaccagg cgttccacgg
    11101 cgccaccctg gatgcaatct catcacggct cgactttgac cggctggacc ataccctcca
    11161 ccagcagatc ctcaacttca tccactcttt cctgcgctgc acctgccgtg acgcccccat
    11221 gtgcggctgc ccagagaaga aattctcccg cgagatcctg gaactccgtg aaaacggcct
    11281 tgaccaccgc cagatcgccc agtatctcca ggaggaatat ggcatcgaca tctacccggc
    11341 cgacatcctc tccttcctcg aggactcggt ccatgtcctc gaggcgatac gtgacatcgc
    11401 tgacctccag gcgatgcctg acctctgcaa aaacgcagat acccatatcg aactccttga
    11461 acgttgagca caggctttcc cgaatttttt ataagaacct tcatgtccag agcctgtaca
    11521 ttgacagata ccatggaatg tatgcagtgc aacgacaact tccgtggcca ggattataca
    11581 agggacgata gattttatcg ctgcttccgg caagggcttc ttctccatcc ccttaccggg
    11641 tggactatag cggagatgcc ctgatgatac gttctctcat tgtcacctgg accccacgtc
    11701 ccggtgagcc ccacccctgt gccatctgca gtgattcggg gctctctttt ctcgaactcc
    11761 tctctttagt ccgccctcta cttgaaaggg acggtatccc tgttaccctg gtcgaaaatc
    11821 ttctttttcc gggctctcaa acggaggaaa acgggttcct cctcaacggg cggcccctcg
    11881 aagaacttct tttggagtct gacagggctc agttcctctg ccactcttcg cgatgccagc
    11941 catacgtgtc aggcgtggat atcacgcgga atgagcgcgg tatacgctgc atcagggccc
    12001 ctgagatcct cttcagaaaa gctatactcc gttcccttga agaggcataa aaccctgatt
    12061 catggctggg acaccgatgt ggaagaagac tgaaccaccc ggatgttttt ttcggatact
    12121 ccaatcccgg gtctgcggtt ttcaaggtca ataaagggtt agtgacaatc ctgccaccga
    12181 catttaaggg aaaaggtgaa attatcccat tagattgagg taggttttcc tattgtattg
    12241 tgaatttcag atgataactc cgttcttcaa ggccaatgtg aatagtattt gaaccgccgc
    12301 cggggcgccc ttcgggggcg gcggggtcta tcgcatatga gaagtgagac caagagcccg
    12361 cacttttttc acctggattg tggtaaatga gttttccatt attcctggtc gtgatttgtg
    12421 aacgagattg gtagtgaaaa tgacacttta ccctcaataa aagaaaagcg aggacccggt
    12481 gttacagcgc cccctcttct gactcatcct tcccactttg cctcttttct ttggcgttgc
    12541 gtggtttttc tccctgtccc cttaccgttg cctggtcctg gatctggcgg gagagcgcga
    12601 tgattcccga tgtatcccca agggtcatgg tgtcaaggac cgatgccggg acaatgacga
    12661 gtgtcgcctt ctcttttaac ccctcgtaga gcatgttcat tgccctgagg tgaagtgccg
    12721 ttggattgtc ctggtaggtg cgtgccgcct cttcgaactt ctcggcgacc tggcgctcgg
    12781 agtcgccgag gatgacccgc gcctgccgct cgcgctctgc ctgtgcctgc atcgacatcg
    12841 catcctggag ctcggccggg ataagcacgt cgcggatctc gactgacgag acatggatcc
    12901 cccagtgctc cgcccgcccc tcgatgatcc gctggagttc gagatccagt ttctcccttc
    12961 cctcaagcat ctcggcaagg ttcgacctcc cgatcacctc ccggagggca gtctgtgcgg
    13021 cccagctcac ggcggtccgg taatcctcga cctcgagtgc cgcgcgctgg ggatcgacca
    13081 ctttccagaa gaggactgcc tcaacgtcga ccgggaccgt gtccttcgtg agcgtctttt
    13141 ctgcggtgaa cgatgtggtg atgatgcggg tatctatcca gtaagcaaca gtctcaacaa
    13201 tcggggtgat gataaaaaca ccgggaccat aaagcccctt gtacttccca agcctgagca
    13261 ccacggcctt ctcccactgg tttgcaatct ggagtgccgc ggcaaggtag agcgcaacca
    13321 caagcgctgc gagcgcaatc gcgaagaaga tgacctgtcc agtgaggatg aagagggaat
    13381 acgagaccca gaggcccagc aggaagagta tcaggaagac ggctgcctgg agggggtttg
    13441 gctgcttcat aggtgtgcac tctcggtcct catcccataa tggtatgggt ggtggaacag
    13501 tcctaatcta ttaagaaagg acgtgtttct tcctgggttt atgccggcga cattcaccgc
    13561 ggccgtttaa ccatgtcgaa ggcgtaccgt gcaaccccag ggcccacata gccacacctc
    13621 cggatggagc gtacttcgac accggcactc tcgtattcac tctggattgc cgggacttgt
    13681 gaagccctct tgaaagtgta gaaatgaatg gtccctccct tctttaccat cgggagaatg
    13741 gagaagagtg cctgatcatg cccgtatggg gccgggatga tagcccgatc gaatttggct
    13801 gaaatgtcag ggaggacatc ctcaacatcg ccttcgtaga cgaaaacccg ggccaccacc
    13861 cggttgaggg atacgttctc acgaaggaac ccgcatgcga gcgggttctt ctcaatcgca
    13921 gtcaccaacg caccgcgact ggccggggga atggcaaaag gacccactcc cgcgaatggg
    13981 accagcacct cttcgcccct tctcacctgc atccacaccc gccgccgttc agacgccatc
    14041 ctcgcagaga agaacgtaac ttccacgtcc agcctgtagc agaacccgta ttccctgtgt
    14101 aaggtgatgg tgcagtcaat acccaaaatc acctccaggt cagccac
//
LOCUS       DAOQYR010000057        14107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_4799_length_14107_cov_8.512027, whole genome shotgun sequence.
ACCESSION   DAOQYR010000057 DAOQYR010000000
VERSION     DAOQYR010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4799_length_14107_cov_8.512027"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /gene="argF"
                     /locus_tag="PKM87_07670"
     CDS             complement(<1..914)
                     /gene="argF"
                     /locus_tag="PKM87_07670"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNQ29982.1"
                     /translation="MKRDIISILDVTAGELGKLLDEADRLKQMRKSGIPHPLLPGKAL
                     AMIFEKSSTRTRISFEVGMADLGGSALFLNAQDMQIGRGEEIRDTARVASRYVSGVMI
                     RAYRHDTIREFARYSSVPVINGLSDLEHPCQILADIMTIREHFPDLAELRLGWAGDGN
                     NVCNSLILSTVLTGMEITVATPEGFEPPSHVVDAARRKGGRVTLVRDPRDATRDAHVV
                     ATDTWVSMGTEKEREARLKAFSGFTIDRNLMDIASPDAIFMHCLPAHRGQEVTDEMIE
                     GPRSVVFDEAENRLHAQKALLVFLMQDG"
     gene            complement(911..2203)
                     /gene="purD"
                     /locus_tag="PKM87_07675"
     CDS             complement(911..2203)
                     /gene="purD"
                     /locus_tag="PKM87_07675"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNQ29983.1"
                     /translation="MTMKILVVGGGGREHAISRALSRNNETELYAVMARENPGISRLC
                     RDFLLEKETRVDSVSAFAKKKSVDYAIIGPEAPLEAGVVDRLEADGIPCMGPTRAAAR
                     LETDKAFCRVMMEQHGIAGCPRYRVFRAATDAVGFLEEYDGDLAIKPAGLTGGKGVRI
                     MGEHLDRGGAIEYIRSLGSDIVLEEKLVGEEFTLQAFVDGTHLVPMPLVQDHKRAFEG
                     DEGPNTGGMGSYSMPDHGLPFVTGIDRRKAFGIMQAAVSAMEEAGTPYRGILYGQFMN
                     TRDGPMVIEFNARFGDPEAMNVLSLLESDLPDIIERSIEGTLSRARVRFLEQATVCKY
                     LVPEGYPESPIMGQPVSLGPYEPALLYYANVEDRGGTLYTQSSRTMAFVGMAASLAGA
                     EEIAERAAEGVKGKVRHRRDIGSPALLKKRVDHMRELR"
     gene            complement(2223..2753)
                     /gene="pyrE"
                     /locus_tag="PKM87_07680"
     CDS             complement(2223..2753)
                     /gene="pyrE"
                     /locus_tag="PKM87_07680"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNQ29984.1"
                     /translation="MVNRIAHLMEEYGALEFGDFVLASGARSSYYLDIKKVVTQPAAL
                     KEIAGAIARDYAFEVVAGVAVGGVPLAVAVSLESMKPFAIVRSQEKHHGKAGRIIGDV
                     RGKHVLLVEDVTTSGGSALFGICALRDAGATVNRVVTVVDRESGATALLHENGVALDA
                     LVRASDVIGRREEQQS"
     gene            complement(2747..3247)
                     /locus_tag="PKM87_07685"
     CDS             complement(2747..3247)
                     /locus_tag="PKM87_07685"
                     /EC_number="2.7.7.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
                     synthase"
                     /protein_id="HNQ29985.1"
                     /translation="MLPAYVPNSVAAALGGGRPIDNGRYFSDGRRIFGDGKTYRGFFA
                     GVAGGILAGGMQIWLQDIFPISSLPAQTLLSIVLLAFGALLGDLAKSFFKRRLGKASG
                     ERWPVADQYDLVVGAFVMLAIFDLPWLLQTVTLPVLFWILVLTPLLHKTANVIGYLTG
                     VKDVPW"
     gene            3383..4855
                     /locus_tag="PKM87_07690"
     CDS             3383..4855
                     /locus_tag="PKM87_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ29986.1"
                     /translation="MAVILKKTRSLCPTCNAVLEAAVVEEDGKVWLERSCPEHGHFRD
                     VYWSDAELWKKFERYESLGKGIENPHRHAPPDGCPHYCGICSNHKSSTLLANIDLTNR
                     CNLNCDFCFANARACGYIYEPTFEQLVEMMKLLRAELPVPAPAVQFSGGEPTMREDLP
                     QIIRTAKDLGFPQVQLATNGVKLAQDPSYLQELRDAGLSTLYLHFDGVSRETNSKLAI
                     DKKVIKNSEELLVGVVLVPTIIKGRNDHEVGAIIQFAAEHIRAVRGVNFQPIAFTGAA
                     SEEDVQRERITIPELLGDIEEQTYGVIKKDDFYPVPCVVPFSDFVEAYTGKPQIRFTA
                     HQHCGAATYVFVNNDGLTPVNRMVDVESLLHALTETANKLRKGGTFNKYKALIEAIGD
                     LNESFKKSEHGNSSEFWKLLGKALLFQNFEALREFHWNALFIGTMHFMDRYNYDLSRV
                     QRCCIHYATPDGTLIPFCTYNSGPVYREIVWKKWSAPLPE"
     gene            complement(4915..5850)
                     /locus_tag="PKM87_07695"
     CDS             complement(4915..5850)
                     /locus_tag="PKM87_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330490.1"
                     /note="catalyzes the interconversion of alanine and
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HNQ29987.1"
                     /translation="MRYYPDPGRDIDLLKINRAVEESFAEHARGKVQMPPKVYVTLPH
                     GDFRTMPAYIPSLDIAGVKVVNVHPGNPSRGLPTVMALTVILDVETGHPRAVLNATEL
                     TDLRTGASGAVAAAHLSPRRSVGLGLVGSGRQAKSQVRAIEKELDIHEVLVWSRSEKN
                     AIQLCSEFGHLDCRSASLERVCGCDLLVTTTPSREPLVQDDWVMEGTHINAIGADAPG
                     KEELDPAILMRARVFVDDPVQAVHSGEVNVPIASGLYSEDMIAGTLGQVVIGEKGRMN
                     PDEITVFDSTGLAIQDLAIAAIALESGEGMPLRFP"
     gene            5996..6790
                     /locus_tag="PKM87_07700"
     CDS             5996..6790
                     /locus_tag="PKM87_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNQ29988.1"
                     /translation="MIHIVRKPTDTPGDDSTSMVVKEITRQGGRYSFLDLDRVDPFSS
                     PLSGALIWICGLRQDEHQFEVCHALSVTNRVVNTPLAIFTCASKVLTSTLLVKNGIPT
                     PRTFFTSSGETAADLIARFGKMVTKPVYGFDGNGISLVDSPEQLGAPPYYLQEYVPND
                     RDFRVFVIDGKAVGAISRISDSLTHNIHQGGTGKPVGIDDRMGELAGAAARAVGVDYG
                     GVDLLPSGDDYCVLEVNGTPNWHCMGVNIPGLLAGYLLSQERLLKG"
     gene            complement(6818..7102)
                     /locus_tag="PKM87_07705"
     CDS             complement(6818..7102)
                     /locus_tag="PKM87_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0058 family protein"
                     /protein_id="HNQ29989.1"
                     /translation="MHKEELITLHQMLYEIKDYFESTNSDLKFNQYYSLKINPSQLHK
                     SKMEHKYAIFILGNELANAMKDVEFSASGRISARMKELAVKTLKEMEYIQ"
     gene            complement(7164..8408)
                     /gene="dnaG"
                     /locus_tag="PKM87_07710"
     CDS             complement(7164..8408)
                     /gene="dnaG"
                     /locus_tag="PKM87_07710"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase DnaG"
                     /protein_id="HNQ29990.1"
                     /translation="MYSSDTTKYMIHIHLEAEGVVEKPDVVGAVFGQTEGLLGEDLDL
                     RDLQRTGRLGRIDVQVTSKKGETKGEIYIASSLDRAETAILAASFETIDRVGPCVARV
                     RVESIEDIRVTKRRQVISRARELLMDAFEEGTIDSNVLIDEVRESMRMEKISTFGEDK
                     VPAGPNVADSDAIIVVEGRADVLNLLRFGIKNAVAVEGTRISRTIVDLCEKKTATAFF
                     DGDRGGDLILRELMQVAEIDYVAYSPRGKSVEDLGRKEIVKALRNKIPADYVREELSG
                     GEEGEASDLSTTSLPHENGSRKSRESDLSQKKRENGKRSEPPASPAPRTLTDHLEAMK
                     GSRSARFLSRDFTILRDVSLQEVERALEEMNGDATGLLLDGEVNQRLLDTVAGKGLEY
                     IAAKEYKGIIKRPLSIKILKIA"
     gene            complement(8569..8895)
                     /locus_tag="PKM87_07715"
     CDS             complement(8569..8895)
                     /locus_tag="PKM87_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNQ29991.1"
                     /translation="MEELCDALTASRDGTVISLEVSAGSKTERFPAGYNPWRKSIGCL
                     VRAPAVEGKANAAVIRLLASFFSLPQEKVQILSGATSSQKRILLAGMHPDQVQKILES
                     APSRGR"
     gene            complement(9057..10355)
                     /locus_tag="PKM87_07720"
     CDS             complement(9057..10355)
                     /locus_tag="PKM87_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ29992.1"
                     /translation="MQARCDLVLTNVLQPGGRVADLSLSGGMVCHVGAGCTAHETIDC
                     RGMTVLPGAVDMHVHMRGRDQGYKEDWASGSMSALAGGVTVVVDQPNTIPPLTTPLSF
                     SQRVAEAREASRCHFAINAGYVPGCDVPALWREGAMAFGELFAAASSYAEGLTPGDLG
                     MALEVVAALGGLCTIHAEEVSAGLDSDLHSHHALRTPEGEARAVRRVMDLNTSGCRLH
                     FCHMSCPGSIRNACNSTVEATPHHLFLSLEDFSSLDARGKVNPPLRPVPVRMELLSAW
                     DRIDVLASDHAPHTVPEKAQPFERAPSGIPGVETMVPLALAYCRAKQIPLSSLIEKTS
                     WKPCEILGIKRAGFQVGDRADFAIYPPGDVAIEAEMLHSKAGWTPFEGKRAVFPGTVI
                     MDGEAVFRDGTFFPGHPSWFPGKGFMGTKLPRNSADTAQP"
     gene            10626..11990
                     /locus_tag="PKM87_07725"
     CDS             10626..11990
                     /locus_tag="PKM87_07725"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNQ29993.1"
                     /translation="MRSIAIILCVLCFIAVGIGCVGGEDTPVDNVNIDTTYTPAEGMA
                     YREQIGGDVGYFSISSVPSGADVIFDGKFLGETPVVTDVYATGSPSHSISISKYGYNT
                     WTRTYNQNPPPGQTIYVNADLQPVVQTGTIEVSSSPSGAIARLDGGMTVTTPGTFRLV
                     TPGYHTVEISKAGYFPYSTSVSVGAGGVSSVSASLSPMQTTGSLRVTSSPSGAEVYVD
                     EIYRGYTPLTVGSLSSGRHAVRLHLSGYQDYTRNVDITTGSEAYVSASLTPVYQPTTG
                     DILVSSVPDGAAIFLDGNYRGKTLQGNPFDITGVSPGTHTVALMKGGYQDYTTTVAVS
                     AGRTSTVSVSLTQGSVPSTTGEITAQSSPSGADVYLDNVYKGFTPLTIQEVTTGSHVV
                     TFRMQGYSDAQYTVQIAAGQTTPVMGLLAPVPTTAPTRSPLSPVVLVGALAILGILFV
                     ARRR"
     gene            12068..12241
                     /locus_tag="PKM87_07730"
     CDS             12068..12241
                     /locus_tag="PKM87_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ29994.1"
                     /translation="MDPKIINILLVVVGFFLLITGIMQVMAVPGMMDYLSIAIGVLLI
                     ITAIVGFWKGKVV"
     gene            complement(12388..13866)
                     /locus_tag="PKM87_07735"
     CDS             complement(12388..13866)
                     /locus_tag="PKM87_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNQ29995.1"
                     /translation="MKRLETLAVIAHDMGIIFKFLGVITLVPFVVLFIFEEWEMVVPL
                     ASVPLTFLLLGLLISRIPRREYVPQISVALAAVALIWLAIAVVGALPFVIGVHMSWTD
                     SIFEAMSGWTGTGFTVIGTLDSTPKTLLFWRSFMQWVGGVGVIAFGIAMLSRSSLMQH
                     QLYRSEGRSEELMPSVVSTGRRMWVIYLFLTVVFTGMVMLSGVPLWDALNLTMVSIAT
                     GGFSLHDAGMAYYHNVVLEGLLIPVMIAGALPFKLYFLMYRGKLRPFFRDRVVHLILA
                     LALVGSAVVTLDLHIFNNFPLETAVRQGFFCTISGLTCTGLQNSDLHWVAGPLIVITI
                     LMMIGGAAGSTSGGIKANRVILGYEGLVWWFRRFFARGKEMVPFRHEGKVIPTRISEV
                     ELSKNMLIIVLYVLIIFVATVIGLHLAATDFKVHEVVFEEVSTMSNVGLGLGYLVPTS
                     PLSTKWIFIFLMWIGRLEIIPVLILAMGLVRGFEARVKGSRK"
BASE COUNT         2987 a         4279 c         3867 g         2974 t
ORIGIN      
        1 tccccgtcct gcatcaggaa cacgagcagg gctttctggg cgtgaaggcg gttctccgcc
       61 tcgtcaaaga ccacgctccg cggcccctcg atcatctcgt ccgtgacctc ctggccgcga
      121 tgggccggca ggcagtgcat gaagatcgca tccggcgatg cgatgtccat cagattgcgg
      181 tcaatggtaa atccgctgaa tgccttcagc ctggcctccc gctccttctc agtgcccatg
      241 gagacccagg tgtcggttgc caccacgtgt gcatcccggg ttgcgtcccg cgggtccctg
      301 accagggtga ccctccctcc tttccttcgt gccgcatcca ccacgtgcga gggtggttca
      361 aacccttcag gggtggctac ggtgatctcc atgccggtga ggaccgtcga gagtattagc
      421 gagttgcaga cattattccc gtctccggcc cacccgaggc gcaactccgc gagatccgga
      481 aagtgctccc ggatggtcat gatgtcggca agtatctggc acgggtgttc gagatcagag
      541 aggccgttaa tcacggggac agaggaatac cgggcgaact ccctgatggt gtcgtgccga
      601 taggccctga tcatcacccc cgacacgtac ctcgatgcca ccctcgcggt atcacgtatc
      661 tcctcccccc tcccgatctg catgtcctgg gcgttgagga agagggcgga tcccccgagg
      721 tctgccatcc ccacctcaaa cgagatccgg gtccgggtgg aggacttctc aaagatcatt
      781 gcaagggcct ttcccgggag gagtggatgg ggaatcccgg acttccgcat ctgtttcagc
      841 cggtctgcct catccagcag cttcccgagt tcccctgccg tcacgtccag aatagagatg
      901 atatcgcgtt tcatcgaagt tccctcatgt gatccacgcg tttcttcagc agggctggag
      961 aaccgatgtc cctgcggtga cgaaccttcc ccttcactcc ctctgcggca cgctcggcga
     1021 tctcctctgc acccgcgagg gaggctgcca tccccacaaa cgccatggtc cgggaggatt
     1081 gggtatagag ggtgccgccc ctgtcctcca cgttggcgta atacaggagc gccggttcgt
     1141 atggtccgag cgagacaggc tggcccatga tcggggattc gggatatcct tccggcacaa
     1201 ggtacttgca gacggtggcc tgttccagga accggaccct ggcacgagaa agggttccct
     1261 ctatgctcct ctcaatgata tccgggagat cgctctcaag gagcgagagg acgttcatcg
     1321 cctcagggtc tccgaacctg gcattgaact ctattaccat cggaccatcg cgggtattca
     1381 tgaactgccc gtagagaatc ccgcggtacg gtgttccggc ctcttccatg gccgaaaccg
     1441 cagcctgcat gatcccaaac gccttgcgcc ggtcgatccc ggtcacaaag gggagcccat
     1501 ggtctggcat ggagtaagac cccatcccgc ccgtattcgg gccctcgtca ccctcgaatg
     1561 ccctcttgtg atcctggaca agaggcatgg ggacgaggtg tgtcccgtcg acgaacgcct
     1621 ggagtgtgaa ctcctctcct acgagtttct cctcgagcac gatgtcggat cccaggctcc
     1681 tgatgtattc gatcgccccc cccctgtcca ggtgctcgcc catgatccgg accccctttc
     1741 ccccggtgag tcctgcaggc ttgattgcca ggtccccatc atattcttcc aggaacccaa
     1801 ccgcgtctgt tgcagcccgg aatacccggt accgggggca cccggcaatc ccatgctgct
     1861 ccatcattac acggcagaac gccttgtctg tctcgaggcg ggctgccgct ctcgtgggcc
     1921 ccatgcaggg gattccgtcc gcttcgagcc tgtccacgac acccgcctcg agcggggcct
     1981 cgggcccgat aattgcataa tctaccgatt ttttcttggc aaacgccgat acagaatcga
     2041 cccgggtctc tttctccagg agaaagtccc ggcagagccg cgagatcccc ggattttcac
     2101 gggccatgac ggcatagagt tctgtctcat tgttgcggga gagtgcacgg gaaatggcat
     2161 gctccctccc cccacccccc acaaccagga tcttcatagt catgtatcgg aaccggtact
     2221 cttcacgatt gctgttcttc tctcctgccg atgacatcgg atgccctgac aagtgcgtcc
     2281 agtgcaaccc cgttctcgtg aaggagcgca gtggcacccg attccctgtc aaccacggtc
     2341 acgactcgat tcacagtcgc acccgcatcc cgaagggcgc agattccaaa gagtgcggat
     2401 ccaccggaag tcgtcacgtc ctccacgagg agtacatgtt tccccctgac atctccgatg
     2461 atccttcctg ctttcccatg gtgcttctcc tggcttcgga cgatggcaaa gggcttcata
     2521 ctctcgagag agacggccac cgcaaggggg actcccccga ccgcaacccc cgccaccact
     2581 tcgaacgcat agtcccttgc gatagctcct gcgatctctt taagggccgc cggctgtgtc
     2641 accactttct tgatgtcaag ataatacgag ctccgggcac ctgaagccag gacaaaatcc
     2701 ccgaattcca gggctccgta ctcttccatg agatgagcga ttctatttac catggtacgt
     2761 ctttcactcc ggtaaggtag ccgatcacgt ttgcggtctt atggaggagg ggagtgagca
     2821 cgagtatcca gaagaggacc gggagcgtga ctgtctgcag gagccatgga aggtcgaaga
     2881 tcgcgagcat tacaaatgcc ccgaccacca ggtcatactg gtcggcaacc ggccagcgct
     2941 caccgctggc cttcccgagc ctccgcttga agaaactctt ggcgaggtct ccgagcagag
     3001 ccccgaatgc caggaggaca atggagagca atgtctgggc gggaagagaa gagatcggga
     3061 atatgtcctg cagccagatc tgcatccctc ctgcaaggat tcctcccgcc acccctgcga
     3121 agaacccacg atacgtcttt ccatccccaa agatcctcct cccgtccgaa aaatacctgc
     3181 cattgtcgat cggcctgccg ccacccagtg cagctgcaac agagttggga acatatgcag
     3241 gcagcatgca ccacaaagcg gctaccaatg ctattatgac ggaatcagga ctaatactat
     3301 gctctccaga caattgtaat aaagagtagt tataagcatt aagatatccc agaagatatc
     3361 aattcttggg tggattggtg acatggccgt tatattgaag aagaccagga gtttgtgccc
     3421 gacctgcaac gcggtgctgg aggcagccgt ggttgaggag gatgggaagg tatggttgga
     3481 gcgctcctgc cccgagcatg gtcatttccg ggatgtatac tggtctgacg cagaattatg
     3541 gaagaagttc gagcggtacg aatccctggg gaagggaatc gagaatcccc accgtcacgc
     3601 acccccagac ggctgtcccc attactgcgg gatatgcagc aaccacaagt ccagcacgct
     3661 ccttgcaaat atcgacctca ccaaccgctg caacctcaac tgcgacttct gttttgccaa
     3721 tgcacgggcc tgcggttaca tatacgaacc cacgttcgag cagttggtcg agatgatgaa
     3781 gctgctccgg gcagagttgc cagtccccgc acccgcagtc cagttttcgg gaggggaacc
     3841 cacaatgcgg gaggatctcc cccagatcat ccgcaccgca aaggatctgg gtttccctca
     3901 ggtccagctg gcaacgaacg gcgtcaagct ggcgcaggac ccctcatacc tccaggaact
     3961 ccgcgacgcg ggcctctcga ccctgtatct ccactttgac ggcgtgagcc gggaaaccaa
     4021 ctcgaagctg gccattgaca agaaggtcat aaagaacagc gaggagctcc tggtaggggt
     4081 ggtgctggtg cccactatca tcaaggggag aaatgaccac gaggttgggg caatcatcca
     4141 attcgcggca gaacacatcc gggcagtcag gggcgtaaac ttccagccca tcgcttttac
     4201 aggcgctgcg agcgaggagg acgtgcagcg tgagcgaatc accatccccg agctcctcgg
     4261 agacatcgaa gaacagacct atggggtgat aaagaaagac gacttttacc ccgtgccatg
     4321 cgttgtccca ttctcggatt tcgtggaggc ctacacgggg aagccccaga tacgtttcac
     4381 cgctcaccag cattgcggcg cggcgactta cgtctttgtc aacaacgacg ggctgacgcc
     4441 tgtaaacagg atggtggatg tggagagcct tctccatgca ctcacagaaa ccgccaataa
     4501 gctcaggaaa ggcggcacat tcaataagta caaggcactc atcgaggcta tcggagatct
     4561 gaatgaatct ttcaagaaga gcgagcacgg gaacagttcc gaattctgga aactcctcgg
     4621 aaaggctctc ctcttccaga acttcgaagc ccttcgcgag ttccactgga atgccctctt
     4681 tatcgggacc atgcatttca tggaccggta taactacgat ctctcgcgcg tgcagcgctg
     4741 ctgtatccac tatgccaccc cggacggcac gctgattccc ttctgcacct acaatagcgg
     4801 gcccgtctac agggagatcg tctggaaaaa gtggagtgct ccccttcccg aataaggggg
     4861 ccatcttttt tccgggcccg gcctccccct tgttttttat ggcttgcggc ctgatcaggg
     4921 gaacctgagt ggcatccctt caccggattc cagtgcgatc gcagcgatgg caagatcctg
     4981 gatcgcaagt ccggtcgagt caaacaccgt gatctcatca gggttcatcc gtcccttctc
     5041 cccaatgacg acctggccaa gggtccctgc gatcatatcc tcggaataga gcccgctcgc
     5101 gatcgggacg ttcacctcgc cggaatggac cgcctgcacg ggatcgtcaa caaaaactct
     5161 tgcccgcatg agaatagccg ggtccagttc ctccttcccg ggggcatccg ctccaatggc
     5221 attgatatgc gtcccttcca tgacccaatc atcctggacc aggggttccc tcgaaggggt
     5281 cgtggtgacc agaaggtcgc acccgcaaac cctttcaagg cttgcgctcc tgcaatcaag
     5341 gtggccgaac tccgagcaca gctggatggc attcttctcg ctcctgctcc agaccaggac
     5401 ctcgtggata tcgagctctt tctcgatggc ccgcacctgc gattttgcct ggcggccgga
     5461 gcccaccagc ccgaggccga cagacctgcg gggggaaaga tgcgctgccg ccaccgcacc
     5521 cgatgcccca gtcctcaagt cggtcaactc cgttgcgttc aggacggcac gcgggtgtcc
     5581 tgtctccacg tcaaggatga ccgtgagcgc catcactgtg ggaagacccc tcgacgggtt
     5641 gccggggtgg acattcacga cctttacgcc ggcgatgtca agagagggaa tataggcagg
     5701 cattgtccgg aaatcaccat ggggaagggt tacgtacact tttggcggca tctgcacttt
     5761 tccacgggca tgctctgcaa acgactcttc aacggccctg tttatcttca ggagatcgat
     5821 gtctctcccg gggtccggat aatagcgcat gcttgctggt acggtgccag gaaagaataa
     5881 tgtgatggca tggatgccct gccagactcg attgtacggc tcaattccag tgagtataag
     5941 tcacacttat accctccaga ctccaacggg atcaccgtac aaggggcatc ataccatgat
     6001 ccatatcgta cgaaagccta ccgatacccc cggtgacgac tcgacctcca tggtggtaaa
     6061 agagatcacc aggcagggag gccgttattc cttccttgac cttgaccggg tcgacccgtt
     6121 cagcagtcca ctctcgggtg cccttatctg gatctgcgga ctccggcagg acgagcacca
     6181 gttcgaggtc tgccatgccc tctcggtcac caacagggtg gtcaacacgc cgctcgcaat
     6241 cttcacctgc gcaagcaagg tcctcaccag caccctcctc gtcaagaacg gcatcccgac
     6301 accccggact ttcttcactt cttcagggga gacggctgca gacctcatcg cccggttcgg
     6361 gaagatggtc acaaagcccg tgtacgggtt cgatggaaat gggatctccc ttgtagattc
     6421 tccggagcag ctcggagctc caccctatta cctccaggaa tacgtgccga atgacaggga
     6481 cttccgcgta tttgtcatcg acggcaaggc agtgggagcc atctcccgga tctctgattc
     6541 cctgactcat aacattcatc agggcgggac aggaaagcca gtcgggattg atgaccggat
     6601 gggtgaactt gcaggggcag ctgcgagagc cgtaggggtg gactatgggg gtgtcgacct
     6661 cctcccgtcc ggtgacgatt actgtgtact cgaggtgaac gggaccccca actggcactg
     6721 catgggagtg aacatacctg ggctcctggc ggggtatctg ctctcgcagg aaagactcct
     6781 gaaaggatga atgcgaagga cgaggaaaac agggtgctta ttggatatat tccatctctt
     6841 ttaatgtctt cacggcaagc tctttcatcc tggccgatat acgtcccgac gcggagaact
     6901 ccacgtcctt catggcgttt gcaagctcgt tacccaggat aaagatggcg tatttatgct
     6961 ccatcttgct tttatgaagt tgcgaggggt tgattttaag agaatagtac tggttgaact
     7021 tgagatctga attcgtggac tcaaaatagt ccttgatctc atagagcatc tggtgcagtg
     7081 tgatcaattc ctctttgtgc atcctctcac ctccagaaga tgattgttgc actggggata
     7141 aataggtaac ccctgaaaaa aaattaagcg attttaagga tttttatcga aagcggacgc
     7201 tttataatgc ctttatactc ctttgccgcg atatactcaa gacccttccc ggcgacggta
     7261 tccaggagtc tctggttgac ttccccgtca agtaaaagcc cggtggcatc accattcatc
     7321 tcttcgaggg ctcgttccac ctcctggagc gagacatcac ggaggatggt aaagtccctt
     7381 gagaggaacc gtgcacttct ggaccctttc atggcttcga gatgatcggt gagggtacgc
     7441 ggggcgggtg atgcgggagg ttcggatcgc ttaccgttct cccgcttttt ctgcgagaga
     7501 tcgctctccc tgctcttcct ggagccattc tcatgcggaa gggaggtggt ggagagatcg
     7561 gaagcctcgc cctcttcgcc cccggaaagc tcctcccgga cataatctgc cgggatcttg
     7621 ttccgaagcg ccttgacaat ctcttttcgg ccaaggtcct ccacgctctt tccacgcggc
     7681 gagtatgcca cgtaatcgat ctccgcaacc tgcatgagtt cgcggaggat gagatctcca
     7741 ccccggtccc cgtcaaaaaa tgcggtcgcg gtcttcttct cgcagaggtc gacgatcgtc
     7801 ctggagatac gggtgccctc aacggccacc gcgttcttga tgccaaaacg gagcaggttg
     7861 aggacatccg cccttccctc gaccacgatg atggcatcag aatcagcgac attggggccg
     7921 gcagggacct tgtcctcccc gaatgtagag atcttctcca ttctcatgct ctccctgact
     7981 tcgtcgatca gaacattgct gtcgatggtc ccctcctcga acgcgtccat caggagctct
     8041 cttgctctcg aaatgacctg cctgcgcttt gtgacccgga tatcctctat cgattccacg
     8101 cgcaccctcg caacacaggg acccacgcgg tcgatggtct caaacgatgc ggcaagtatg
     8161 gccgtctctg cccggtcgag ggatgacgca atgtagatct cccccttggt ctcgcctttc
     8221 ttgcttgtca cctggacgtc tatccttccc aggcgacccg tcctctgtaa gtcccgcagg
     8281 tcaaggtctt cgccgagcaa cccttcagtc tggccgaata cggcccccac tacgtcaggt
     8341 ttctcgacca ccccctccgc ctcgaggtga atatgaatca tatacttggt agtatcgctg
     8401 gagtacatac accccactcc gtgaatcgta atctgtcacc caaccaatgc cttgctgaat
     8461 tgccgcacct gtcatgtgac aaatataagt tagatattat aatatatgaa tatatttttg
     8521 cgaaacctgc accaggaccc aagtacaggg gattgcggtg ccatgagatc acctcccgcg
     8581 gctgggagca ctttccagga ttttctgtac ctggtcaggg tgcatacccg caaggagtat
     8641 ccttttctgg gaggatgtgg ctcccgagag gatctggacc ttctcttgag gcagggaaaa
     8701 gaacgacgcg aggagcctga tgaccgctgc attggccttc ccttccacgg caggcgcacg
     8761 caccaggcag ccgatgctct tcctccatgg attgtatccg gcaggaaacc tctcggtctt
     8821 tgaaccggca gatacttcaa gggagatgac agtcccatcc cggctcgcgg tcagggcatc
     8881 gcatagctct tccacgtata agaaaggagg tgtcgcccgg cattaacaac tgcggatcac
     8941 tggtgggctg accccgtccc tcccctgttt atacccattg ccgggcggtg cctgtcgcac
     9001 aaccgggttg tcccgtagtt catatccagg cgtccgccaa tcgatcaccc ggggacctat
     9061 ggctgtgctg tgtcggcact attcctgggg agttttgttc ccataaagcc tttccccggg
     9121 aaccatgagg ggtggcccgg gaaaaaggtt ccatcccgga agaccgcctc cccgtccatg
     9181 atcacggttc ctggaaagac cgccctcttc ccctcgaacg gggtccagcc tgccttgctg
     9241 tggagcatct ctgcctcaat cgccacgtcg ccgggagggt agatcgcaaa gtccgccctg
     9301 tcgcccacct ggaaaccagc tcttttgatt cccaggatct cgcacggttt ccaggaggtc
     9361 ttctcaatca gcgaggagag ggggatctgc tttgcccggc agtaggccag tgccaggggc
     9421 accatggtct ccacgcccgg gattcccgaa ggcgcgcgtt cgaaaggctg cgctttctct
     9481 ggcacggtgt ggggggcatg atccgatgcc agcacgtcta tcctgtccca ggcggagagg
     9541 agttccatcc ggaccggaac cgggcggagg ggcgggttca ccttccccct tgcatcgagc
     9601 gatgagaagt cctcgagcga gaggaacagg tggtggggcg ttgcctccac ggtggaattg
     9661 caggcgttcc ggatcgatcc agggcagctc atatggcaga agtggagcct gcatccgctc
     9721 gtgttcaggt ccatgacacg ccggactgcc ctggcctccc cctccggagt gcggagcgcg
     9781 tgatgagagt ggaggtcgct gtccagcccg gccgaaacct cctcggcgtg aatggtacag
     9841 agtcccccca gtgcggcaac gacttcgagc gccatcccaa ggtcgccggg tgttagtccc
     9901 tcggcataac tcgaagcggc ggcaaagagc tccccaaagg ccatcgcccc ctctctccac
     9961 agggctggga catcgcatcc cgggacatac cccgcattga tggcgaagtg gcacctggag
    10021 gcttcccgtg cttcggccac cctctgcgag aacgacaggg gggtggtaag gggggggatt
    10081 gtgttcggct ggtccacgac aaccgtgact cctcctgcga gcgcactcat gctcccgctt
    10141 gcccagtcct ccttgtatcc ctggtccctg cctctcatgt ggacatgcat gtcgaccgca
    10201 ccggggagga ccgtcatccc gcggcaatcg atcgtctcgt gggctgtaca cccggctccc
    10261 acgtggcaga ccattcctcc cgagagcgag aggtctgcca ctcttccgcc gggttggagg
    10321 acatttgtga ggacaagatc gcagcgggct tgcatgggac gtctttcacc tgggtgttgg
    10381 tgcctgaacg agatgatact tggcccccat ttctcctctt cgtcaagttc acgaacaggg
    10441 aagaatgccc tttcttctta tttggggagg cgttccgggg agaggcactc cggactgctt
    10501 cagacagtct tcctatcggg aagaaggtat ttgccccgta attttcccat ttctcccagg
    10561 ggagagaaga attaatcagg agaggcttcg aagactcatc acagaagatt agagaggaaa
    10621 ataagatgag atcgatcgca atcattctct gcgtactctg ctttatcgcg gtaggcatcg
    10681 ggtgcgtcgg tggagaagac actcccgtcg acaacgtgaa cattgataca acctacaccc
    10741 ccgcagaagg gatggcgtac cgcgagcaga tcggcggaga tgtggggtac ttctccatca
    10801 gctcagtccc cagcggagcc gatgtcatct tcgacgggaa attcctcggg gagaccccgg
    10861 tggtcaccga tgtgtacgcg accggctctc cttctcactc catttccatc agcaagtacg
    10921 ggtacaatac ctggacaagg acctacaacc agaacccgcc cccgggccag accatctacg
    10981 tgaacgcaga tctccagccc gtggtccaga ccgggaccat cgaggtgagc tcatcgcctt
    11041 ccggcgccat cgcgaggctc gacgggggca tgacagtgac aactcccggg acattcaggc
    11101 tcgtgacgcc gggataccac acggtcgaga tctccaaggc aggatacttc ccctacagca
    11161 cctctgtgag cgtcggtgcg ggaggagtct ccagtgtgag cgcatcgctc tctcccatgc
    11221 agaccacggg atccctccgg gtcacgtcat cgccatcagg ggcagaggtc tacgtggacg
    11281 agatctaccg gggctatacg ccgcttactg ttggttccct gagttccggc aggcatgccg
    11341 tccgccttca cctctcgggg taccaggact atacccgcaa cgtggatatc acaaccggga
    11401 gcgaggccta tgtatctgcc tcactgaccc cggtgtacca gcccactacc ggtgacatcc
    11461 ttgtcagctc tgtacccgat ggcgctgcca tcttcctcga cgggaactac cgcggaaaga
    11521 ccctccaggg caacccgttc gacatcacgg gggtctcgcc cggtacccat actgttgcgc
    11581 tgatgaaggg cgggtaccag gactacacca ccacggttgc ggtgtctgcg ggaaggacct
    11641 caacggtctc ggtctcgctc acccaggggt ccgtgccctc caccacgggc gaaatcacgg
    11701 cgcagtcatc tccctccggg gctgacgtct acctcgacaa cgtgtacaag ggatttaccc
    11761 cgctcaccat ccaggaggtc acgacaggct cacacgtggt cacgttcagg atgcagggct
    11821 attcagatgc gcagtatacc gtccagatcg cggccgggca gacgaccccc gtcatggggc
    11881 tgcttgcgcc ggtccccacc acggccccca ccaggtcgcc tctctcaccg gtcgtgttgg
    11941 tcggcgcgct tgcgatcctg ggtatcctgt tcgttgcgag gagaaggtaa ggatcactcc
    12001 tttttttcca aattcccccg ccaaaaggag tggaattatt tttataccaa cttattgaga
    12061 gagtaccatg gatccgaaga tcatcaacat cctcctcgtg gtggtaggct ttttcctgct
    12121 catcacaggc atcatgcagg tcatggccgt cccagggatg atggactact tatctatcgc
    12181 cattggcgtt ctcctcatca tcaccgcgat cgtcgggttc tggaagggaa aagtggtctg
    12241 aaggtccatg cctctctctg gagtgaggag aaccggcact gatcgctccg gaatccccct
    12301 taaaaaaaat ctggtcattc ccttgttccc cccgagatcc tgcagccacg aacctgcaaa
    12361 gaaaggattt tttttcataa aaaccgctca tttcctgctc cccttcaccc tggcttcgaa
    12421 tcccctgacg agtcccatgg caaggataag gacagggatg atctccagtc ttccaatcca
    12481 catcaggaat atgaatatcc acttggtcga gagcgggctt gtggggacca ggtatccgag
    12541 gccgagcccc acgttgctca tcgtggagac ctcctcgaac accacctcgt ggaccttgaa
    12601 atcggttgcc gccaggtgga ggccgatgac agtagccacg aagatgatca ggacatagag
    12661 gacgatgatc agcatgttct tggagagttc gacctccgag atccgcgtgg ggatgacttt
    12721 tccttcatgc cggaacggga ccatctcctt tcccctcgca aagaagcgcc ggaaccacca
    12781 cacgaggcct tcgtacccaa ggatgacccg gttggccttg atgcccccgg aagtgctccc
    12841 ggccgccccg ccgatcatca tcagaattgt gatgacgatg aggggtcctg cgacccagtg
    12901 aaggtccgag ttctggagac cggtacaggt caacccggat atcgtgcaga agaagccctg
    12961 ccggaccgcg gtctcaaggg ggaaattatt gaagatatgg agatcgagag tgaccacggc
    13021 cgatcccacg agcgcgagtg ccaggataag gtggacgacc ctgtcccgga aaaacggcct
    13081 tagcttcccc ctgtacatga ggaaatacag cttgaacggc aacgcacccg cgatcatgac
    13141 cgggatgaga agtccctcga ggaccacgtt gtgatagtat gccatcccgg catcatggag
    13201 tgaaaatccg ccggtggcaa tgctgaccat cgtcaggttg agggcgtccc agagggggac
    13261 ccccgagagc atcaccatgc cagtgaagac aaccgtgagg aagaggtaga tcacccacat
    13321 ccgccttccc gtggagacca cgctcggcat cagctcctcg gaccgcccct cggaccggta
    13381 gagctggtgc tgcatgaggc ttgacctgga gagcatggcg atgccaaacg cgatcacccc
    13441 aaccccgccg acccattgca tgaacgagcg ccagaagagg agggtctttg gggtggagtc
    13501 gagtgtcccg atcaccgtga acccggttcc tgtccacccc gacatggcct cgaagatgct
    13561 gtccgtccag ctcatgtgga caccgatcac aaacgggaga gcgcccacta ctgcaatggc
    13621 aagccagatg agtgccactg cagcaagggc gaccgagatc tgcggaacat actcccttct
    13681 tggaatgcgg gatatgagaa gaccgaggag caaaaatgtc agcggaacgg aggccagcgg
    13741 caccaccatc tcccattctt caaatataaa tagcacaacg aagggaacga gagtgatcac
    13801 tccaaggaac ttgaagatga tccccatgtc atgggcgatg acggccagtg tctccagccg
    13861 cttcatgatg agatgattat caggggtagt ttatcttcat tctgtatgat cgatagggca
    13921 aaatcaacat ggtctccgtt gattacggcg ggatcttccg gaatccgtgc atcactcgca
    13981 tcatttcggt tgatgcaccg cagacactct ctcccaacgt gttccctgcc ttatcatcgt
    14041 actggagaat ggtatgatgt tttaaaggag agcatttctt caaaaaaaag gaatgcgagg
    14101 gatggga
//
LOCUS       DAOQYR010000058        14019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_4833_length_14019_cov_9.769407, whole genome shotgun sequence.
ACCESSION   DAOQYR010000058 DAOQYR010000000
VERSION     DAOQYR010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 14019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14019
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4833_length_14019_cov_9.769407"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..1471)
                     /gene="secY"
                     /locus_tag="PKM87_07740"
     CDS             complement(38..1471)
                     /gene="secY"
                     /locus_tag="PKM87_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNQ29996.1"
                     /translation="MGELLDRMEPLLAAMPTVKGPEGHVHFKNKLVWTAGILILYFVL
                     TNIPLFGLDPGSQDIFFYWRALLAGASGSLVQLGIGPIVTASIVLQLLKGADLIHIDT
                     SEMKGQIQYMGLQKLLIFVMIIIEAAPNLVGGFLMPDPVIADAFFGGNLFLVSFIIFL
                     QVCIGGVLIVFMDEVVTKWGIGSGVGLFIIAGISQAIINGFINWAPVSDQYPVGFFPR
                     IAAVIMDGANFLQYMGTDMIAFITTIAIFLIIVYVESTRIEIPLAHAQVRGARARFPV
                     KLIYASVLPMILVRVLQANIQMIGLFLNNVGITIFGTFQGATPVSGLMWYLAPINGPS
                     DWMWWLYDLGHAPWEVLTRMGIDILVVVIGGAIFALFWIKTAGMDSKDVARQIQHSGM
                     SIPGYRRNPQVLEKYLDRYIPRVTVIGGVFIGVLSVFANLFGVIGAVSGTGLLLTVSI
                     TYRLYEEIASQQIMEMYPFMRTFFGKE"
     gene            complement(1492..1917)
                     /locus_tag="PKM87_07745"
     CDS             complement(1492..1917)
                     /locus_tag="PKM87_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL15m family ribosomal protein"
                     /protein_id="HNQ29997.1"
                     /translation="MPVNKRSKFRGSRTCGGGTHKNRRGAGNRGGRGRAGHRDHRFSH
                     FLVYGEVHNGKHGFVNQTSAAQNALSVGRLDEMVDELIAAGMASREGDAVVILTDQIG
                     IDKILGNGKVTRKMHVSARAFSGQAKQKIEAMGGQALVI"
     gene            complement(1922..2383)
                     /locus_tag="PKM87_07750"
     CDS             complement(1922..2383)
                     /locus_tag="PKM87_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNQ29998.1"
                     /translation="MYAIVQVRGVVNTRRDIKDTLKMLRLHHINHCVLVPDTPEYLGM
                     IRKVKDFVAYGEVDDETVETVLRTRGRVEGNRPLTDEYVKENSTYPGIRDFARALASG
                     EARLSDIPGVKPVLRLHPPRKGYRTIKRTYPQGGALGYYGREINELLYKMR"
     gene            complement(2383..3000)
                     /locus_tag="PKM87_07755"
     CDS             complement(2383..3000)
                     /locus_tag="PKM87_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNQ29999.1"
                     /translation="MAYEKEEWVPLTGLGKMVVSGELTSIDQVLESGRPIKEPGIVDA
                     FLPDLEDEVLDITMVQRMTDSGRRVKFRAVVAVGNRNGYIGFGQGKDSQVGEAIKKAI
                     VDAKTNLIKVRRGCGSWECGCDTTHSIPMMVEGAAGSVRVTLKPAPQGIGLVTGEISK
                     KVLELAGIRDVWTFSRGQTRTTINFAKATFNALKATNMIRTGGQE"
     gene            complement(3002..3529)
                     /locus_tag="PKM87_07760"
     CDS             complement(3002..3529)
                     /locus_tag="PKM87_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNQ30000.1"
                     /translation="MATGPRYFVPFRRRKEGKTNYYARAKLVVSDRPRMVVRRTNRHI
                     IIQLVTAEMDGDRTLVSANSGELSGFGFTGSTSSTPAAYLTGALFAVKALNADYESAI
                     LDIGLHRATRGARIFAALKGAVTAGLDVPHGEEILPDDDRVKGAHIAAYAPDRAGDLV
                     QNVEQTMDAIMKELE"
     gene            complement(3531..3983)
                     /locus_tag="PKM87_07765"
     CDS             complement(3531..3983)
                     /locus_tag="PKM87_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HNQ30001.1"
                     /translation="MTDLSTQKRVSASIMKCGVHRVWFDPERTSDIQNAISREDLRTL
                     IDEDAIRALQPRGNSRGRAREKMAKRSYGHCKGPGRRKGAAGARSPRKREWIKKIRAI
                     RKTLADLRDTDEINPHLYRTLYRQAAGGQFRSVAHLKAHLALISGRMK"
     gene            complement(3976..4419)
                     /locus_tag="PKM87_07770"
     CDS             complement(3976..4419)
                     /locus_tag="PKM87_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32e"
                     /protein_id="HNQ30002.1"
                     /translation="MAEEKIRRIRARAEKSARFRRDGEGKKERLADTWRRPRGLHNKQ
                     RIQKKAKGPMPTPGYGSPADVRGLHPSGYRDVLVHTPDEIEGLDPALDAVRIAAAVGA
                     KKRGFIQEKAMAAGLKVLNPKDLSKKKGAPVEEEPEADEEVEEND"
     gene            complement(4425..4949)
                     /locus_tag="PKM87_07775"
     CDS             complement(4425..4949)
                     /locus_tag="PKM87_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNQ30003.1"
                     /translation="MATERTVRIPKGVKVEIDGPLVRVKGQKGQLERNFRFPQIAMKV
                     EGDEVVISTGADRKRVVAMVGTIQAHVANMCKGVSEGFEYRMKVVYSHFPIQLKLSGN
                     RLEINNFLGEKKPRYALIRDGVTAKVGNDEIVLSGINRETLGITSANIEDATRIRNRD
                     PRIFQDGIYRIQRG"
     gene            complement(4968..5360)
                     /locus_tag="PKM87_07780"
     CDS             complement(4968..5360)
                     /locus_tag="PKM87_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNQ30004.1"
                     /translation="MARLNTIADGMCALKNAADGGKPAVIIEPASKLLGAMFRIMQEA
                     GYINGFEFIEDGRGGQFMVHLNGRINRCGAISPRYPVKISDLEYWETQFLPGKNFGIL
                     LLSTSKGVITHEQARREGVGGELLGYVY"
     gene            complement(5377..5532)
                     /locus_tag="PKM87_07785"
     CDS             complement(5377..5532)
                     /locus_tag="PKM87_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNQ30005.1"
                     /translation="MGGEKTKKYGRGANECKMCGRKQALVRRYNIMFCRQCFREWAQK
                     MGFKKMN"
     gene            complement(5534..6040)
                     /locus_tag="PKM87_07790"
     CDS             complement(5534..6040)
                     /locus_tag="PKM87_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNQ30006.1"
                     /translation="MNPMRDVFVEKVVVHMGVGEAGDKLTKAVDLMKTITGGTPVRSN
                     AKQTNTTFGIRKGAPISCRVTLRGEKATNFLNTALGIVDRRISGSQIDRRGNFSFGVE
                     EHTDFPGMSYDPQIGIYGMDINVVLARRGIRISRRATEQRKLPQKQQVGQEDAIAFLK
                     SGHQVEVL"
     gene            complement(6037..6762)
                     /locus_tag="PKM87_07795"
     CDS             complement(6037..6762)
                     /locus_tag="PKM87_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
                     /protein_id="HNQ30007.1"
                     /translation="MSDHLKRLNAPKSWHIARKTEKFIARTSPGPHNANAMPVAVWLR
                     DHMRFASNMKEVKQILVQKDVIINGKPCRNPRMGIGIFDIISVPRINKHYRILRDKRG
                     RHKTIEIDAESAKSRLAKIRNKTIVPGGKVQLNLRFGANVLADNTYKPGDSVVISLEP
                     ENRFAILDHFPFAVGNMAMVIGGKHSGIVARIVGIEKVPGSVPNRVLLEDPESKVRFE
                     TIDEYIYMVGREQPALTEWGIEE"
     gene            complement(6762..7127)
                     /gene="rplX"
                     /locus_tag="PKM87_07800"
     CDS             complement(6762..7127)
                     /gene="rplX"
                     /locus_tag="PKM87_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNQ30008.1"
                     /translation="MVRIESTQPRKQRKARYTAPTHARGRFLNAPLAPDLRKQYNTRS
                     VRVVVGDTVKVLRGESAGTEGPVDEVDTRKSRIVVQGVSIPKSDGTEMPRPVSPSNVQ
                     ITKLNLKDPRRAASLGESE"
     gene            complement(7138..7536)
                     /locus_tag="PKM87_07805"
     CDS             complement(7138..7536)
                     /locus_tag="PKM87_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNQ30009.1"
                     /translation="MKAKQSNIPRALSTGSRMTCADNTGAREVQIVSVFGYHGVRRRQ
                     PKMGLGDIATVSVKKGTPDMRRKLVRAVVIRQKKEMRRPSGLRVGFEDNAVVVVDEKN
                     EPRGTEIKGPVAREVAERFPKIGSMATIIV"
     gene            complement(7533..7859)
                     /locus_tag="PKM87_07810"
     CDS             complement(7533..7859)
                     /locus_tag="PKM87_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNQ30010.1"
                     /translation="MANNIGLSVRQPERECDDANCPFHGTLPVRGQVITGKVVSDRMI
                     GTVVVERAYLHYVGKYNRYEKRNSKIHAHNPPCIQARTGDTVRIAECRPLSKTTTFVV
                     VEVQPS"
     gene            complement(7859..8143)
                     /locus_tag="PKM87_07815"
     CDS             complement(7859..8143)
                     /locus_tag="PKM87_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component 1"
                     /protein_id="HNQ30011.1"
                     /translation="MISPRNILRHELIGLDVTVVEASNPALTGLSGRIIDETRNTLVI
                     HGDRGKKRVQKHRTRFRVTLPDGVLVEIDGSALVMAPERRISALHKTRGK"
     gene            complement(8145..8348)
                     /gene="rpmC"
                     /locus_tag="PKM87_07820"
     CDS             complement(8145..8348)
                     /gene="rpmC"
                     /locus_tag="PKM87_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNQ30012.1"
                     /translation="MAIFRAQEVRQLSDVELQEQMEKLKIELIQHNGKVSAGGSTENP
                     GRIRELRRTIARMMTEANSRRRG"
     gene            complement(8350..9063)
                     /locus_tag="PKM87_07825"
     CDS             complement(8350..9063)
                     /locus_tag="PKM87_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNQ30013.1"
                     /translation="MAVERKFVAEGVRKARVEKHLKKELKRAGYGGMDLQRTPLGTQV
                     TIFAEKPGIVIGKGGKVVRQLTQDLATDFAVESPQIEVQQVQNPSFNAQIMAERLANA
                     LERGWYFRKAGQSIIRRVMEAGALGCEVVIAGKLTGARARVQKFSDGYIKHCGEPSNT
                     IVEKGYAVAVKKLGVIGVQVKIIPAGAKIPDHFEVTAKREKPRVAEEIAVTSVGEEVL
                     EEIPEGPDDFNEEKLLGRK"
     gene            complement(9063..9524)
                     /locus_tag="PKM87_07830"
     CDS             complement(9063..9524)
                     /locus_tag="PKM87_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNQ30014.1"
                     /translation="MARTLYSQDVDGDNVARGKANELPISPKHAMEIASFIKFKRTGE
                     AISYLEQVIDQKKAIPFRRFNRKVAHKRGLEGWDAGRYPKKASHAYIRLLQSVVKNAE
                     YIGLDIEKLEIVHVSANRGRGFRGMFPRAMGRATPKRRETVNIEIVVREVQ"
     gene            complement(9530..9943)
                     /locus_tag="PKM87_07835"
     CDS             complement(9530..9943)
                     /locus_tag="PKM87_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNQ30015.1"
                     /translation="MAKKTQKRLPRRREEFTYRGFRLDDLRAMGMSELVPLMPSRARR
                     KVNRGLTRGEEHLLTQFRKGDGAIRTHLRDMIITPEMVGRPVEIHNGKEFVKVEIQPE
                     SLFHYLGEFAPTRKKVSHGSAGIGATRSSKYVPLK"
     gene            complement(9955..10671)
                     /locus_tag="PKM87_07840"
     CDS             complement(9955..10671)
                     /locus_tag="PKM87_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNQ30016.1"
                     /translation="MGHRITTQSRGKGGPTYRAPSHRYKAALRHVSSGEITVHGKVED
                     IEHDPARHAPIALVRLENGSKVYMLITEGMGVGDSVAWGAEADVRNGNTLPLGAIPTG
                     TFICNIEAQPNDGGKFVRSGGVQAVVVDKSEGRVGVTMPSGKTKWFNDRCRATVGIVA
                     GGGRGEKPFVKAGKKFHKMRNTASNWPRVRGVAMNVIDHPFGGGGHQHPGRPKTVARG
                     TSPGRAVGHMAARKTGLSKK"
     gene            complement(10681..10929)
                     /locus_tag="PKM87_07845"
     CDS             complement(10681..10929)
                     /locus_tag="PKM87_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNQ30017.1"
                     /translation="MVLRYPFVTEKAMMMLENQNKLQFVVESNATKNQIKREIERAFA
                     QEVSHVSTMMAMDGRKKAVISFANEKAAEEILSRLGIM"
     gene            complement(10931..11677)
                     /gene="rpl4p"
                     /locus_tag="PKM87_07850"
     CDS             complement(10931..11677)
                     /gene="rpl4p"
                     /locus_tag="PKM87_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNQ30018.1"
                     /translation="MKAQVYSLEGTVAGEIELPPVFSEEFRPDLIRKAVIALQSTRRQ
                     PHGTYPYAGILSSAQSWGSGRGVAQLPRIKGGSRAAKIPQARGGREAHPPVVQKVLVR
                     HINKKEKQKAFRSALAATVSEDLVRARGHAFTCQVPLVIEDRFGELGKTSEIISALAA
                     VGVLQDVERAKASKKVRAGRGKMRGRRYKQRKSLLIVTADTPLRAAGNLAGVDVATVE
                     QLNCELLAPGTQAGRLTVWTESALMKLGGQ"
     gene            complement(11685..12698)
                     /locus_tag="PKM87_07855"
     CDS             complement(11685..12698)
                     /locus_tag="PKM87_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNQ30019.1"
                     /translation="MPKINQPRKGSLAFSPRKRAKSPVPKYQSWPPYQGNPMLQGFAG
                     YKVGMTHVIMVDDHKNSPTEGKDIMVPVTVVEIPAMKVAAIRAYGRDSYGKHPLTEVW
                     AGDLDDALGRRITPPKEYDGTTAREAIKDAITRGIVAELFAVMHTTPYDVPGVPKKVP
                     DIMEVRVAGGSPEDQFAFLEGLLGKEVDLKSVIQPGAYADVTAITTGKGTQGPVKRWG
                     IKLRKRKHSRGGKKRHVGNLGPWNPHHVRWQVPQMGQMGYQQRTEFNKRILKIGENGA
                     EISPAGGFLHYGMLKNPYVLIKGSIPGPVKRLVRIRPAMRQGEHVARQPAIEFVSMES
                     KQG"
     gene            complement(13307..13711)
                     /locus_tag="PKM87_07860"
     CDS             complement(13307..13711)
                     /locus_tag="PKM87_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfx family DNA-binding protein"
                     /protein_id="HNQ30020.1"
                     /translation="MKDGLLTDRQKEVLRYRKQGLTQQQIADIIHTSKANVCTIEKSA
                     VENIKRAKETLEFLYTLDATHLCTIPAGTDLLEVAASIYQEAEKIGIKVKYDTISLIN
                     KMRDSAPERFKARFVKENIDVFINEEGDLYFG"
     gene            13928..>14019
                     /locus_tag="PKM87_07865"
     CDS             13928..>14019
                     /locus_tag="PKM87_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F420-dependent methylenetetrahydromethanopterin
                     dehydrogenase"
                     /protein_id="HNQ30021.1"
                     /translation="MVVKVGVAKLGNIASGVMAELLLDERADRE"
BASE COUNT         2346 a         4457 c         3918 g         3298 t
ORIGIN      
        1 gggacaccgg tcacgatcac tctccttcca gtcattctca ctccttcccg aaaaatgtcc
       61 gcatgaaggg gtacatctcc atgatctgct ggcttgcgat ctcctcgtag agccggtaag
      121 tgatggacac cgtcagtaaa agacccgtcc cgctcaccgc cccgatcaca ccgaagaggt
      181 ttgcaaacac ggagaggacc ccgatgaaca cgccaccgat tacagtgacc ctcgggatgt
      241 acctgtcgag gtacttctcc agcacctggg ggttcctccg gtatcccggg attgacatcc
      301 ccgagtgctg gatctgacgg gccacgtcct ttgagtccat tcccgcggtc ttgatccaga
      361 acagggcgaa gattgcgcca ccgatcacca cgaccaggat atcgatcccc atcctggtga
      421 gcacctccca gggagcgtgc ccgaggtcat acagccacca catccagtcc gacggaccgt
      481 tgattggggc gagataccac atcaggccgc tcacgggagt ggcgccctgg aacgtgccaa
      541 agatggtgat cccgacatta ttgaggaaga gcccgatcat ctggatgttc gcctgcagca
      601 cccggacgag aatcatcggc agcacgctcg cgtagatcag ttttaccggg aacctcgccc
      661 ttgccccccg tacctgtgca tgtgcgagcg ggatctcgat cctcgtggac tccacataca
      721 cgatgatcag gaagatggcg atggtggtga tgaacgcaat catgtcagtg cccatgtact
      781 ggaggaaatt ggcgccgtcc atgatgaccg cggctatccg ggggaagaac cctacagggt
      841 attgatcgct gacaggcgcc cagtttatga acccgttgat aatggcctgg gatatcccgg
      901 caatgatgaa gagcccgacc cctgatccta tgccccactt ggtgaccacc tcgtccatga
      961 atacgatcaa cacccctcct atacacacct gcaggaagat gatgaacgag acgaggaaca
     1021 ggtttccacc gaagaatgca tctgcgatca cgggatccgg catcaggaaa ccgcccacta
     1081 ggttcggggc ggcctcgatg atgatcatca cgaagatgag aagcttttgc aggcccatgt
     1141 actggatctg gcctttcatc tcgctcgtgt cgatgtggat caggtcggcc cctttcagca
     1201 gctgcagcac gatggatgcg gtcacgatgg gaccgatacc aagctgcacg agagacccgc
     1261 tggcacctgc aaggagggcg cgccagtaga agaagatatc ctgggaaccc gggtcgaggc
     1321 cgaagagcgg gatgttggta agcacaaaat acagaatcag gatccctgca gtccagacca
     1381 gcttgttttt gaagtggacg tggccttcag gcccttttac agtgggcatc gctgcgagca
     1441 gcggttccat tcggtccaga agttctccca ttttttcacc acaaaaaggg attagataac
     1501 cagggcctgg ccacccatcg cctctatctt ctgttttgcc tgtcctgaga atgcacgcgc
     1561 ggacacgtgc atcttccgtg tgacttttcc gttgccaagt atcttatcga taccgatctg
     1621 atccgtgagg atgaccactg cgtcaccctc gcgggaggcc atgccggccg caatgagctc
     1681 atcgaccatc tcgtcgagcc tgcccacaga gagggcattt tgggctgccg aagtctggtt
     1741 gacaaaacca tgcttgccgt tgtgcacttc cccatacacc aggaaatggg agaaccggtg
     1801 gtcgcggtga ccggcgcggc ccctgccgcc ccggttccct gcacctctcc tgttcttgtg
     1861 ggtgcctcca ccgcatgtcc ttgagccacg gaatttggat cgcttgttca ctggcattgc
     1921 ttcacctcat cttgtacagg agctcgttga tctccctgcc gtaatatcca agggccccgc
     1981 cctgcgggta tgtcctcttg atggtgcggt atcccttccg gggagggtgg agcctgagta
     2041 ccggtttaac gccgggaata tccgacaagc gggcttcccc tgatgcaagg gcccttgcaa
     2101 agtccctgat gccagggtag gtggaatttt ccttcacgta ctcgtcggtg aggggcctgt
     2161 tcccttcaac cctgcccctg gtgcgaagga cagtctcgac ggtctcgtca tccacctcgc
     2221 cataggccac gaaatccttg accttcctta tcatcccgag gtactctggg gtatccggga
     2281 ccagcacgca gtggttgatg tggtgcaggc ggagcatctt caaggtatcc ttgatgtccc
     2341 gccgtgtatt cactaccccg cggacctgca cgattgcgta cattactcct ggcctcccgt
     2401 gcggatcatg ttagtcgcct tcagcgcgtt gaaggttgcc ttggcgaagt tgatcgtggt
     2461 cctggtctgg ccgcgggaga acgtccatac gtcacggatg cccgccaatt ccagcacttt
     2521 cttgctgatc tcccctgtca cgaggccgat gccctggggc gccggcttga gggtgacccg
     2581 cacactgcct gccgcacctt ccaccatcat ggggatggag tgggtggtat cgcagccgca
     2641 ttcccagctg ccgcaacccc ttcgcacttt tatcaggttg gtcttggcat ccacgatcgc
     2701 tttcttgatc gcctctccca cctgggagtc tttgccctgc ccgaacccta tgtagccatt
     2761 cctgttcccg accgccacca cggcgcggaa cttcacccgg cggccggagt cggtcatccg
     2821 ctgcaccatg gtgatgtcaa ggacctcgtc ctcgaggtca gggaggaagg catcgacaat
     2881 gccgggctcc ttgatggggc ggccgctctc cagcacctgg tcgatgctgg tcagctcgcc
     2941 cgagaccacc atcttcccaa ggccggtcag cgggacccat tcctctttct catatgccat
     3001 attactccag ctccttcatg atggcgtcca ttgtctgttc cacgttctgc acgaggtccc
     3061 ctgcacggtc cggcgcatag gccgcgatgt gcgcaccttt cacccggtca tcgtcgggga
     3121 gaatctcctc gccgtgcggg acgtcgagtc ctgcggtcac cgcccctttc agtgcggcga
     3181 atatgcgggc tccccgcgtt gcacggtgca ggccgatatc gaggattgcg gactcataat
     3241 cggcattcag ggctttcacc gcaaagagcg ccccggtgag gtatgcggcc ggggtgcttg
     3301 aggtcgaccc cgtgaatcca aaaccggaga gctcgcccga gttggcggag accagtgtac
     3361 ggtcgccgtc catctctgca gtgaccagct ggatgatgat atgcctgttc gtgcggcgga
     3421 ccaccatccg gggccggtcg gatacgacca gttttgccct ggcgtagtag ttggtcttgc
     3481 cctccttcct cctgcggaag ggaacgaagt agcgcggtcc tgttgccatg ttacttcatc
     3541 ctcccgctta tgagcgcaag gtgggccttc aggtgcgcga cgctcctgaa ctgccccccg
     3601 gcggcctgcc ggtagagcgt gcggtacagg tggggattga tctcgtcggt gtcgcggaga
     3661 tccgcgagag tcttgcggat cgcgcgaatc ttcttgatcc actcgcgctt cctggggctc
     3721 ctggcgccag ccgccccctt gcgccgcccc gggcccttgc agtgcccgta tgaccgcttg
     3781 gccatcttct cccttgctct tcccctgctg ttgcccctgg gctggagggc ccggattgca
     3841 tcctcgtcga tgagggtgcg gaggtcttcg cgggagatcg cgttctggat atcggaggtc
     3901 cgctcggggt caaaccagac ccggtggacc ccgcacttca tgatcgatgc ggataccctc
     3961 ttctgggtgg agagatcagt cattttcttc cacctcctcg tcggcctcgg gctcctcttc
     4021 cacgggtgcc cccttcttct tggaaaggtc tttcgggttg aggactttca gccctgcagc
     4081 catggccttt tcctggatga aacccctctt ttttgccccg accgcggctg cgatgcgaac
     4141 cgcgtcaagc gcggggtcga gtccctcgat ctcgtcaggt gtgtggacca gcacgtcgcg
     4201 gtatccgctg gggtgcagtc ccctgacgtc cgccgggctc ccgtacccgg gagtgggcat
     4261 cggccccttt gccttcttct ggatccgctg cttgttgtga agcccgcggg gcctcctcca
     4321 ggtgtcggcg agccgctcct tctttccctc cccgtctctc ctgaaacggg cggatttctc
     4381 tgccctggca cgaatacgtc tgattttttc ttcagccatg gtcttcatcc cctctgtatc
     4441 cggtaaatgc cgtcctggaa gatgcgggga tcgcggttcc ggatccttgt cgcgtcctcg
     4501 atgttcgcag aggtgatgcc gagcgtttcg cggttgatcc cggagaggac gatctcgtcg
     4561 ttgcccacct tcgcggtgac tccgtccctg atcagggcat accgcggctt cttctcgcca
     4621 aggaagttat tgatctcgag cctgtttccc gaaagcttga gctggatcgg aaagtggctg
     4681 tagaccactt tcatgcggta ctcgaacccc tcggataccc ccttgcacat gtttgcaacg
     4741 tgcgcctgga tggttcccac catggcgacg accctttttc ggtccgcgcc ggtggagatg
     4801 acgacttcat ctccttcgac cttcatcgcg atctggggga accggaagtt cctctcgagc
     4861 tgccccttct ggcctttcac cctcacaagg ggcccgtcaa tctctacctt cacacccttt
     4921 gggatgcgca ctgttcgttc tgttgccatt gtattcacgc accccctcta gtagacatac
     4981 cccagaagct ccccgcccac accctcgcgg cgggcctgtt cgtgggtgat gacccccttc
     5041 gaggtggaga ggaggagtat gccaaagttc ttccccggca ggaactgggt ctcccagtac
     5101 tccaggtcgg agatcttcac cgggtaacgg ggggagatgg cgccgcaccg gttgatcctc
     5161 ccattgaggt gaaccatgaa ctggcctccc cgcccgtcct cgatgaactc gaagccgttg
     5221 atgtacccgg cctcctgcat gatgcggaac atcgcgccga gcagtttgct cgccggctcg
     5281 atgatgaccg cgggcttgcc tccgtctgcc gcgttcttga gggcgcacat gccatctgcg
     5341 attgtattaa gtcttgccat aagcctgacg ctccctctag ttcatctttt taaagcccat
     5401 cttctgtgcc cactcgcgga agcactggcg gcagaacatg atgttgtagc gccgcaccag
     5461 ggcctgtttc cggccgcaca tcttgcactc gtttgcaccg cggccgtatt tcttggtctt
     5521 ttctccgccc atttcagagc acctccacct ggtggcctga cttgaggaat gcaattgcat
     5581 cctcctggcc tacctgctgt ttctggggaa gcttgcgctg ctccgtcgct ctccgggata
     5641 tgcggattcc ccggcgggca aggacgacat tgatgtccat gccgtagatg ccgatctggg
     5701 ggtcgtagct catccctggg aaatcggtgt gctcctcgac gccgaacgag aagttcccgc
     5761 ggcgatcgat ctggctcccg ctgatccgcc tgtccacgat gccgagcgcc gtgttgagga
     5821 agttcgtcgc cttttcgccg cgaagggtga cccggcagct gataggggcg cctttccgga
     5881 tgccgaacgt agtgttggtc tgcttggcat tgctgcgcac cggcgtgcct cccgtgatgg
     5941 tcttcatcag gtccacggcc tttgtcagct tgtcaccggc ctcgccgacc cccatgtgca
     6001 ccacgacctt ctccacaaag acatcgcgca ttgggttcat tcttcaatcc cccactcggt
     6061 gagggccggc tgttcgcggc ccaccatgta gatgtactcg tcgatggtct cgaagcgcac
     6121 cttgctctcg ggatcttcca ggagcacacg gttcggcacg ctccccggca ccttctctat
     6181 ccccacgatg cgggcgacaa tgcccgaatg ctttccccca atgaccatgg ccatgttgcc
     6241 gacagcgaac gggaagtggt cgaggattgc gaaccggttc tcgggttcaa gggagatgac
     6301 cacggagtcc ccgggcttgt aggtgttgtc tgcaagcacg tttgcgccga accgcaggtt
     6361 gagctgcacc tttccacccg ggacaatcgt cttgttccgg atctttgcca ggcggctctt
     6421 tgccgactcg gcatcgatct cgatggtctt gtgccgcccc ctcttgtcac gcaggatgcg
     6481 gtaatgcttg ttgatcctgg ggacagagat gatgtcgaag ataccgattc ccatcctggg
     6541 gttccggcat ggcttgccgt tgatgatgac atccttctgg accaggatct gcttgacctc
     6601 cttcatgttg gaggcaaagc gcatgtggtc gcggagccac accgccaccg gcatggcgtt
     6661 ggcgttgtgc ggtccggggg aggtcctggc gatgaacttc tccgtctttc tggcaatgtg
     6721 ccaggacttg ggggcattga gcctctttag gtggtctgac attattcact ctcccccagg
     6781 gacgccgctc tccgcggatc cttcaggttc agtttggtga tctgcacgtt ggaaggcgag
     6841 acgggtcttg gcatctccgt cccgtccgac ttgggaattg agacaccctg gacgacgatg
     6901 cgggacttcc ttgtgtccac ttcgtccaca ggtccttctg ttccggccga ctcgcccctg
     6961 agcaccttga ccgtgtctcc caccaccacg cgcacgctgc gggtgttgta ctgcttccgg
     7021 aggtccggcg caagcggtgc attgaggaaa cggccccggg cgtgggttgg cgcggtatac
     7081 ctggccttcc tctgctttct tggttgtgta ctctcgatcc tcaccatggc caatccctca
     7141 cacgataatg gtggccatgg agccgatctt cgggaaccgc tccgccactt ccctggcgac
     7201 cgggcccttg atctccgtcc cgcgcggctc gttcttctcg tccaccacga cgacggcgtt
     7261 gtcctcaaaa ccgacccgca gcccgctcgg tctgcgcatc tccttcttct gccggatcac
     7321 cacggcccgc acgagtttgc ggcgcatgtc gggggtgcct tttttcacac tcacggtggc
     7381 gatatcgccg agccccatct tgggctgccg cctgcgcacg ccgtgatacc cgaacactga
     7441 cacgatctgg acttcccggg cgccggtgtt gtccgcacag gtcatccggc tgccggtgga
     7501 gagtgctctc gggatgttcg actgctttgc cttcatgatg gctgcacctc caccacgaca
     7561 aaggtggtgg tctttgagag cggcctgcac tctgcgatgc gcacggtatc accggtcctc
     7621 gcctgaatgc agggggggtt gtgggcatgg atcttgctgt tccgcttttc gtagcggttg
     7681 tatttgccca cgtagtgcag gtatgcccgc tctaccacca ccgttccaat catccggtcg
     7741 ctcacgactt tgccggtgat cacctggccg cgcaccggta aagtgccgtg aaacggacaa
     7801 tttgcgtcgt cacattccct ctcgggctgc ctgacgctca atccgatgtt gtttgccatt
     7861 atttccctct ggtcttatgc aatgcgctga tacgcctctc tggtgccatg accagcgcgg
     7921 agccgtctat ctccaccagg acgccatccg gaagggtgac gcggaagcgg gtcctgtgtt
     7981 tctggaccct cttctttccg cggtcaccat ggatgaccag tgtattcctg gtctcatcga
     8041 taatccgccc cgagaggccg gtcagcgcgg ggttgctcgc ctccaccacg gtgacatcca
     8101 ggccgatgag ctcgtgcctg aggatgttcc ggggggagat catcttaccc gcgtctcctg
     8161 ctgttcgctt cggtcatcat ccgggcgatg gtccggcgca gttccctgat ccggccggga
     8221 ttctcagtgg aaccccctgc gctgaccttc ccgttgtgct ggatcagctc tatcttgagc
     8281 ttctccatct gctcctgcag ctccacgtcc gagagctgcc gcacttcctg tgcacggaat
     8341 atggccatcc tacttcctcc ccagcagttt ctcctcgttg aagtcatcgg gcccttccgg
     8401 gatctcctcg aggacctcct cgccgacaga tgtcactgca atctcctcgg cgacgcgggg
     8461 cttctccctc tttgcggtca cctcgaagtg atcggggatc ttcgcaccgg cggggatgat
     8521 cttgacctgg accccgatga cgcccagttt cttgaccgca accgcgtaac ctttctcgac
     8581 gatggtgttc gagggctctc cgcagtgctt gatgtagcca tcggagaact tctggacccg
     8641 ggcgcgggct ccggtcagtt tcccggcgat gaccacttcg cacccgagcg ctcctgcctc
     8701 catcacgcgt cggatgatgc tctggccggc cttcctgaag taccagccgc gttcgagggc
     8761 gtttgccagc ctctctgcca tgatctgggc attgaaactg ggattttgaa cctgctgcac
     8821 ctcgatctgg ggtgactcaa ccgcgaaatc ggtagcaagg tcctgggtaa gctgcctgac
     8881 gacttttcca ccctttccga tcacgatacc gggtttctcc gcgaatatcg tgacctgggt
     8941 ccccagggga gtgcgctgca ggtccatgcc gccgtacccc gcccgcttca gctccttctt
     9001 taagtgcttc tccacgcggg cctttcgtac gccttcagcc acgaattttc tttctactgc
     9061 cattactgca cttccctgac cacgatttcg atgttcacgg tctccctgcg cttgggggtc
     9121 gccctgccca tggccctcgg gaacatgccc cggaagccgc ggccgcggtt ggccgagaca
     9181 tgcacgatct ccagtttttc aatgtcgagg ccgatgtact cggcattctt tacgactgac
     9241 tgcaggagcc ggatgtatgc atgggacgcc ttttttggat agcgtcctgc atcccatcct
     9301 tcaaggccgc gcttgtgggc caccttccgg ttgaacctcc ggaaggggat ggccttcttc
     9361 tggtcgatga cctgctcgag gtaggagatg gcttctcccg tgcgcttgaa cttgatgaag
     9421 ctggctatct ccatggcatg cttcggggag atgggaagct cgttcgcctt tccccgcgcc
     9481 acgttgtcgc catcgacatc ctgtgaatag agtgttcttg ccatatccat cacttcagcg
     9541 gcacgtactt gctcgaccgg gtcgcaccga taccggcgct cccgtggctc accttcttcc
     9601 ttgttggggc gaactcgccc aggtagtgga agagggattc gggctggatc tccactttga
     9661 cgaactcctt cccgttgtgg atctccaccg gccttcccac catctccggc gtgatgatca
     9721 tgtcacgcag gtgagtgcgg atcgcaccgt cgcctttccg gaactgtgtg aggaggtgct
     9781 cctcgccccg ggtgaggcca cggttcacct ttcggcgggc gcgggacggc atcagtggta
     9841 ccagttcgga cattcccatc gcacgcaggt cgtccagccg gaacccccgg taggtgaact
     9901 cctcgcgccg cctcggaagt ctcttctgtg tcttctttgc cattgtctta cctctcactt
     9961 cttagaaagc cccgttttcc tggcagccat gtgcccgaca gcgcgtcctg gcgaggtccc
    10021 gcgggcgacc gtcttcggcc gccctgggtg ctggtgtcct ccgcctccga agggatggtc
    10081 gatcacgttc atcgccactc ccctcacgcg gggccagttg gacgcggtgt tccgcatctt
    10141 gtggaacttc ttcccggcct tgacgaaggg cttctcgccc ctgcctccgc cggcaacgat
    10201 cccgaccgtt gcccggcagc ggtcgttgaa ccatttggtc ttgccgctcg gcatggtcac
    10261 gccgacccgg ccctcgctct tgtcgaccac aactgcctgt acgcccccgg aacggacgaa
    10321 cttacctccg tcgtttggct gcgcctcgat gttgcagata aacgtccctg tggggattgc
    10381 accgagcgga agggtgttcc cgttccgcac atcggcttcc gcaccccatg cgacactgtc
    10441 gccgacgccc atgccctcgg tgatcagcat gtagaccttg ctgccgttct cgagcctcac
    10501 cagggcgatc ggtgcgtgcc ttgccgggtc atgctcgatg tcctcgacct tcccgtgcac
    10561 cgtgatctcg ccgctgctca cgtgccgcaa tgcggccttg taccggtggg agggagcccg
    10621 gtaggtcgga cctcctttcc cccggctctg tgtggttatg cgatgtccca tcttccccac
    10681 ctacatgatg ccaagccggc tgagaatctc ctcggccgct ttctcgttgg cgaaactgat
    10741 taccgccttc ttcctgccat ccatggccat catggtggac acatggctga cttcctgcgc
    10801 aaaggcccgc tcgatctcgc gcttgatctg gttcttggtg gcgttgctct cgaccacgaa
    10861 ctgaagcttg ttctggttct cgagcatcat catggccttc tctgtgacga acgggtaacg
    10921 aagtaccata ttactgccct ccgagtttca tgagcgcgct ctcggtccac accgtgagcc
    10981 ggccggcctg cgttcccggc gcaagaagct cgcagttcag ctgctccacc gtggcgacgt
    11041 ccacgccggc aaggttccct gcagccctga gaggggtatc cgcggtcacg atcaggaggc
    11101 tcttccgctg cttgtagcgg cggcctctca tcttcccgcg tccggcacgg accttcttgc
    11161 tggcctttgc ccgctccacg tcctgaagca cccccaccgc tgcaagggca gagatgatct
    11221 cgctcgtttt gccgagctcg ccgaaccggt cctctatcac caggggcacc tggcaggtga
    11281 acgcgtgtcc gcgggccctg accaggtcct cgctgacagt cgcggcaagg gctgacctga
    11341 atgccttctg tttctctttc ttgttgatgt ggcggaccag caccttctgg accaccggcg
    11401 ggtgagcctc ccggccaccc ctggcctggg ggatttttgc agcccgtgaa ccgcctttta
    11461 tcctcgggag ctgggctacg ccgcgtcctg atccccagct ctgcgctgat gagagaatgc
    11521 cggcatacgg gtaggtccca tgaggctggc gccgggtgct ctgcagggcg atgaccgcct
    11581 tcctgatcag gtcggggcgg aactcttcag agaataccgg cggaagttcg atctccccgg
    11641 caactgttcc ttcgagtgag tatacctgtg ctttcatcgt agtatcaccc ctgcttgctc
    11701 tccatgctca caaactcgat cgccggctgc ctggccacat gctctccctg tctcatggcg
    11761 gggcggatgc gtacaaggcg cttgacaggg ccgggaatgg atcccttgat cagcacgtag
    11821 gggttcttca gcatgccgta atgcaaaaag ccgcctgccg ggctgatctc ggcaccgttc
    11881 tctccgatct tgaggatgcg cttgttgaac tcggtcctct gctggtaccc catctgtccc
    11941 atctggggta cctgccagcg cacgtggtga gggttccatg gccccaggtt acccacatgg
    12001 cgcttctttc ctcccctcga atgctttcgc ttccgaagct tgattcccca gcgcttgaca
    12061 gggccctgcg tcccttttcc ggtcgtaatc gcggtgacat cggcatatgc accgggctgt
    12121 atcacgctct ttaaatcgac ctcttttcca aggagcccct cgaggaacgc gaactgatcc
    12181 tcaggggagc cccctgcaac cctgacctcc atgatgtcgg gcactttctt tgggaccccg
    12241 gggacgtcgt agggcgtggt gtgcatcact gcaaagagtt ctgcgacaat gccccttgta
    12301 atcgcgtctt ttatggcctc tcttgcggtc gttccatcgt actccttcgg aggcgtgatc
    12361 ctcctcccga gagcgtcgtc gaggtcgcct gcccacacct cagtcagagg gtgtttgccg
    12421 taagaatccc ttccgtaggc ccggatcgcg gcgactttca tcgccgggat ctccacaacc
    12481 gtgaccggga ccatgatatc cttcccctcg gtcgggctgt ttttgtgatc atcgaccatg
    12541 atcacgtggg tcatgcctac cttgtagccg gcaaagccct gtagcatcgg gttcccctgg
    12601 taaggaggcc aggactggta cttcggtacc gggctcttgg ccctcttgcg ggggctgaag
    12661 gcaagggatc cttttcttgg ctggtttatc tttggcatgg ttcaatcaat cctgcactat
    12721 atgactcaac tgtggccatc tcctggcctg aaccgagcta atcggttgaa atcgcctata
    12781 tcggccgata tacaccggag atgagtgatg tgtccgggcc cctgccatgc aatctgccgg
    12841 cggttgcccg ggttcggtac cctggctgct gccaaccgat tgccgcagcg gaaaggtggg
    12901 atgatacccg cctttggtgc agtatcccgg aaagacagcc gtttctctgg ctcctggccg
    12961 cctcccctgt gacctgtctc gcgaaagaga tcctgaaact ggcgcgcaac actgccatcc
    13021 tcgtggcagg caccgggcac ataccccaga ggatacgtcc gcccttcacg cctctcttca
    13081 gttccaccat gtcatccggt ggtgattgct caccacatac tcatgcaaag gcacgaagtc
    13141 cctcggaacc cgtgcctccg acctctgatg agactccatc ccctggccgc tatgtgccgg
    13201 gcctggatgg atcctcatat aggcttccat agttatacga gagaaatgaa tataaacctt
    13261 gttccatttc ggagaggacg aaccaccggg ccgatagggg gtcttttcag ccgaagtaga
    13321 ggtccccctc ttcgttgatg aacacatcga tgttctcttt cacaaagcgg gctttgaatc
    13381 gctccggtgc tgagtccctc atcttgttga tcagcgagat cgtatcgtat ttcaccttga
    13441 tcccgatctt ctctgcttcc tggtagatgg atgcggcgac ttcgaggaga tccgtcccgg
    13501 cggggatggt gcagagatga gtggcgtcca gcgtgtataa aaattccagg gtctcttttg
    13561 ccctcttgat gttctcaact gccgacttct cgatggtgca cacattcgct ttcgacgtgt
    13621 ggatgatatc tgcaatctgc tgctgggtaa gcccctgctt ccggtaacgc aggacctcct
    13681 tctgacgatc cgtcagcagc ccgtctttca tgatactgga taggaggatt ggacttaaac
    13741 acttttcgtt aacttcgtca cctgcacctc cgaaacgggg ggaggccagg tatggcaggg
    13801 taggtccact ctccgattac tcattaaaac caaaaatcgc cctgatttgc taaaaaaaag
    13861 atttcaaaac gtttatattc gcaccggcag atagtaacct gttataacca gaaatgaggt
    13921 gtattacatg gttgtgaaag ttggagttgc aaaactgggc aatattgcca gtggtgtaat
    13981 ggccgagctt cttctggacg agagagccga ccgcgagga
//
LOCUS       DAOQYR010000059        13976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_4846_length_13976_cov_8.416565, whole genome shotgun sequence.
ACCESSION   DAOQYR010000059 DAOQYR010000000
VERSION     DAOQYR010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 13976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13976
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4846_length_13976_cov_8.416565"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..506
                     /locus_tag="PKM87_07870"
     CDS             33..506
                     /locus_tag="PKM87_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30022.1"
                     /translation="MHEILFFGVIFGGIFSLFSSLILVHMGMLIDRFPVGDEEERERC
                     IWSPREFTRYAVIFYWPLYIVGAAMLVPGTRIMTVAGIATLFGLVLFMITALIFSVAT
                     YNLMRSKSRGSSSPSIMVSFGKPGSFMRAFRLPAHGRKSHSTRSSQASRAAVVED"
     gene            complement(1099..1443)
                     /locus_tag="PKM87_07875"
     CDS             complement(1099..1443)
                     /locus_tag="PKM87_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30023.1"
                     /translation="MNLSKPGIIGSIFFLISFLTFSAVPVYAEMISSRSSTPRLSEIS
                     KMSAIEKMAANPFSNEIYIPDFGYHDQYISPWGADASKNYESNVKEFGEVIASFLMHK
                     GNSYSEYRSIMR"
     gene            complement(1943..2296)
                     /locus_tag="PKM87_07880"
     CDS             complement(1943..2296)
                     /locus_tag="PKM87_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30024.1"
                     /translation="MNPYTLKGIGSIFLICAFVGFGHISGYGAGFSSTKISPQLNEKL
                     TSLQNPASTDTNIINSLSGIKSLMEQPGTLGALISVRQTRGLIYACTPIDGGGIFCKW
                     VYPDPEIRGDQLRGL"
     gene            complement(2442..3659)
                     /locus_tag="PKM87_07885"
     CDS             complement(2442..3659)
                     /locus_tag="PKM87_07885"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNQ30025.1"
                     /translation="MPIGTLKEIKPEGKTILLRLDLNSPIDPTTRHILDDKRFREHLP
                     TIQALSQSRVVIVTHQSRPGKKDFTTLEAHAERLEALLQRRVKYVDDIFGQCARDAVR
                     SMKNGDVLVLENVRFNAEENLTVKAEDAQKLYLVKKLAQMGDFFVNDAFGTAHRSQPT
                     MVGLPMVMRSVAGLLMEKEVSTLTRVFQNAPRPVCLVLGGTKVDDSISVAEHMLKGNT
                     ADLIIVTGVVANIFLSASGFDIGRPSTQLIQQLKYEGEIARARALLSSFKDRILLPGH
                     VAVKENGKRAEYPVNAIPGEAPVMDAGVDSIAQLSGAIRKCGTVVFNGPAGVFEEPDF
                     ATGTYELLRTASKVEFSIIGGGHTAAVVEKMGIDRNFTHISTGGGACIEFLTGKKLPA
                     VQALERSRELFGG"
     gene            complement(3675..4301)
                     /locus_tag="PKM87_07890"
     CDS             complement(3675..4301)
                     /locus_tag="PKM87_07890"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNQ30026.1"
                     /translation="MALRDIKPTRSELINLKKRIALSERGYKILKMKRDGLILEFFKV
                     LDQAKDTRGDLNERYENAQSMMAVANTVEGAIGVKAAAFSVQEVPDIKVASKNIMGVV
                     VPEIESSKVRKGLNERGYGILGTTSVIDETALSFEELVESIIRSAEIETTMKKLLDEI
                     ESTKRRVNALEFKVIPELTEARDFIKMRLDEMEREELFRLKKIKARNV"
     gene            complement(4308..5699)
                     /locus_tag="PKM87_07895"
     CDS             complement(4308..5699)
                     /locus_tag="PKM87_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNQ30027.1"
                     /translation="MKEYRTITQIAGPLVFVEKTEPVGYGELVNIVLFDGTIKRGQVL
                     DTSDELVVVQVFETTAGIGKDSGIRFTGETIKMPVGRDMLGRILSGGGKPIDGGPEIV
                     PEKRLDITGAAINPYARGSPNDFIQTGISTIDGTNTLVRGQKLPIFSGAGLPHNQIAL
                     QIARQSKVPGSKEAFAVVFAAMGITKEEANYFMQDFERTGALERAVVFLNLADDPAVE
                     RIITPRLALTTAEYLAFDLGMHVLVILTDMTNYCEALRQIGAAREEVPGRRGYPGYMY
                     TDLAGIYERAGIVKGKKGSITQFPILTMPGDDITHPIPDLTGYITEGQIVVSRELHRK
                     GIYPPINVLPSLSRLMNLGIGKGHTREDHKKVSDQMYAAYAEGNDLRGLVAIVGKDAL
                     SERDRMFLEFADIFEGRFVRQGYSEDRTIEDTLTIGWNLLSSLPVEQLVRIDRDLINK
                     YHPKFAQAGKAEG"
     gene            complement(5696..7456)
                     /locus_tag="PKM87_07900"
     CDS             complement(5696..7456)
                     /locus_tag="PKM87_07900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016469 - proton-transporting two-sector
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit A"
                     /protein_id="HNQ30028.1"
                     /translation="MKTEKPKSDTTGVLKRIAGPVVTAVGIDAHMYDVVKVGNEELMG
                     EVIKIQGENIIIQVYEDTSGIRPGEPVINTGLSLAVELGPGLLTSIYDGIQRPLSVLV
                     DKMGDFIQRGVSAPGLDHAKKWEFKPLAKEGATVAPGSIIGEVQETNIVHRVMIPPNV
                     KGGTIKTIKAGMFTVDEVVCTLDNGAQISMMQKWPVRVPRPVNEKMNPTVPLITGQRI
                     LDGLFPIAKGGTAAIPGPFGSGKTVTQQQLAKWSDAEIVVYIGCGERGNEMTEVLTEF
                     PELEDPKSGKPLMERTVLIANTSNMPVAAREASVYTGITIAEYFRDMGYDVSLMADST
                     SRWAEAMREISSRLEEMPGEEGYPAYLAARLSEFYERAGRVKTLAGSMGSVSVIGAVS
                     PPGGDFSEPVTQNTLRIVKVFWALDAKLSQRRHFPAINWLNSYSLYLDSLHDWYDKEV
                     SPEWNTIRSWAMEILQKEAELQEIVQLVGSDALPEAEQITIEVARMIREIFLQQNAYD
                     AVDTFCDMKKQYDMMKAIRQYSDLAYAAQSAGVTPQQILAVKAKNELPQIKFIKDYKP
                     ALDKIQAELEAEFNALRSAA"
     gene            complement(7453..7755)
                     /locus_tag="PKM87_07905"
     CDS             complement(7453..7755)
                     /locus_tag="PKM87_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HNQ30029.1"
                     /translation="MEIAVIGNQEFILGFRLAGIRKTYPVENDEMLLAHINRVLDDSD
                     VGILVLQSSDLKKIPRRLQMTLEESVKPTVITIGAEEGGLSMRERIKRSVGVDLWK"
     gene            complement(7755..8810)
                     /locus_tag="PKM87_07910"
     CDS             complement(7755..8810)
                     /locus_tag="PKM87_07910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HNQ30030.1"
                     /translation="MAEVRAGPVPYIYVCTRMRVRKAKLLPREEYLRMLNMGLSEITR
                     FIEETQYKKEIDELATSFHGIDLIEIALSWNLAKEYQNIQKITPGTLKDLTRAYLRKW
                     DIQNVLTVLRGKLHGERSGKIREVLIPAGELDKHFLDRLIEEPTPERIIEALKGKGVY
                     PLLSRSFEGGVKEEFLPRVENELYKQFYAELVSMAMSGIKGGKQFMDYITLDIDITNI
                     SNMFRFRADQSGEEMMAAIIPGGTISPQQIQRLMYIRDRNEFIDALVPLVKVRVLREL
                     LEKLREEKSIREIEIDLIKVQLAEMESMSKLHPFSIHPILAYLEMKKYEIFNLRALAR
                     GKESNLTPDRIRGYLVI"
     gene            complement(8823..9401)
                     /locus_tag="PKM87_07915"
     CDS             complement(8823..9401)
                     /locus_tag="PKM87_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HNQ30031.1"
                     /translation="MGLEAVLADIREKGQQEMQRIRSESQEETSRVLTAAQERAAKIK
                     QAANDDAERQSGYILSQEISAANLLVKRELLNTQKDLLDQVYERAIVTIAGLPESFHR
                     DAITRLLTEAKQQIPGGIVRVNQRDKALLERILAEKPEFKAFSTGKDAEIDGGVIVES
                     MDGTLQLDYSYRTFLDMIWESGLKDASDLLFG"
     gene            complement(9412..9633)
                     /locus_tag="PKM87_07920"
     CDS             complement(9412..9633)
                     /locus_tag="PKM87_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNQ30032.1"
                     /translation="MVKASQLGMKALGAGIAVGLTGIGTGLAEMAIGSAAVGATAENK
                     DVFGLVLLLTVIPETIVIFGLVVGLLLLF"
     gene            complement(9685..11658)
                     /locus_tag="PKM87_07925"
     CDS             complement(9685..11658)
                     /locus_tag="PKM87_07925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HNQ30033.1"
                     /translation="MLTPEGMTRVLIAATKSQMEPVIREMYRQHLFHIEEFVEKDRIE
                     YEGYRIGTPLPQANETSRELLRLRGITNAFMIRDDQEASQSERQKNAHLKEVVEKELP
                     ILEQEVEELLGRRSRLDTQVKEYEQKISGLQPFRDVPFDLELLRGYKTFSVFSGYILR
                     DISLDIPHEMFTSDTKKAKFVILIVSNQHRGSAEQALLDAQFQAVPIPEENGLAKDRI
                     GFYESQILAVKGELEDINRRLEDLKKEHKNLFLACNELLTADVEMAEAPLRFATTGET
                     FIVEGWVPTAELEKFRQGIHYATGGKVYVYELEGSEIEDIAPTEYHNVGFAKPTEMLM
                     DVYSRPRYTEIDPTLLVSIIFPIFFGIILGDVGYGAILLALSIGLQRFMKGDDGTRLL
                     KVLRNASISSIVFGILFSEFFGFSLPWSPILFSRHLNIGGHAAGHGPEVTSLMIMAIW
                     IGILQITLGRVLGIINHARQDHGSHRVKAVMANLGWIMVLWGILLMLWASFTMPFMPD
                     FTGLPPIVMGLNLSSILGIVLILLGVIFIIRDSALEIVELPTILSHVLSYARLVGVGL
                     SSVAIAMVVNFIAIGLIIEPQLESLSAVGVIIIIVGALVFVIGHLLNTILGMLGGGLQ
                     SIRLQYVEFFTKFYKGGGKKYTPFGMKKRFMED"
     gene            complement(11645..11974)
                     /locus_tag="PKM87_07930"
     CDS             complement(11645..11974)
                     /locus_tag="PKM87_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNQ30034.1"
                     /translation="MKTEVLKNIKATESEYQKMISDALAEKKRNSANAELEADNLIMK
                     ATHDAEEYKKAKLAEARQNAAKKHDEILAEGKRRAAELKERGTRNLGRAADLLVSRFK
                     ERLHAHS"
     gene            complement(12203..12679)
                     /locus_tag="PKM87_07935"
     CDS             complement(12203..12679)
                     /locus_tag="PKM87_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ30035.1"
                     /translation="MQEKMVQIGKDTLLLVRSKSSFLLAGNAGSRFPLCIETHDEEYC
                     QTLEPSHIVAVSAPEGGSVQAAWMLIELVRTYRLPLVVMPRSHPGSRRLRYVVSAGDE
                     ILLSCTIERGTHPEQHLLCSSEEMGGIRLLGTASGILVSGIPDGVDLGVVPVPSLR"
     gene            complement(12695..>13976)
                     /locus_tag="PKM87_07940"
     CDS             complement(12695..>13976)
                     /locus_tag="PKM87_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNQ30036.1"
                     /translation="GDLVSFLSGRVRLYQEEARFEQKQFLSFLSLVAESYVTLFVAGP
                     LFLIIIMVVMGMMGGVAILQLTAVIYLVFPIGSLVFILLIDIISLKGETVERYVRAKW
                     LHEYSDVRVYHREGEEPFFARLVHYDRVRNAREFFRHPFDAFQKNYNLTLFITVPLAI
                     IYVVAVFWVVPQYQDLEVYIDVVDDHVMIALLIVMVPYAFFYEMWRRNVQGMESLIPE
                     FLDRMAGINQVGLTIAQAIGIMVNTNLGLLSYEIKRIKRDIDWGASFTDALIRFEDRI
                     RTPLIARTVTLITKASQMSGSIGDVLAIAASDAKMSDLLKRERLSEMFIYTAIIYLAF
                     VVFVFVVAVISNNFIPILGSVDAGGIPEGTAISNVSAASIMAILRLMFHSVVLQALLS
                     GLIAGQMGEGSIKAGIKHSCIMMVIALVIFNLFI"
BASE COUNT         2949 a         3869 c         3768 g         3390 t
ORIGIN      
        1 ctgccatggt gatatagtgg aggcagacaa tcatgcacga gatactgttc tttggcgtaa
       61 tcttcggggg gatattctcc cttttctcca gtctcatact ggtgcacatg gggatgctga
      121 ttgacaggtt cccggttggg gacgaagagg agagggagcg gtgcatctgg agcccaagag
      181 agttcacgag gtatgcagtg atattctact ggccgctgta tatcgtgggt gccgccatgc
      241 tggtccccgg gacccgcatt atgactgttg caggcattgc caccctcttt ggacttgtcc
      301 tgtttatgat caccgcactg atattttcgg ttgcaaccta caacctgatg agatcgaaga
      361 gccgggggag cagcagtcct tcgattatgg tctccttcgg aaagcctgga agtttcatgc
      421 gcgcgttccg cctgcctgcc catgggagga agtctcattc tacccggtcc tcgcaggcct
      481 cccgggcggc ggttgttgag gattagggcc tcccccttct ctctcttttt ctctgcacac
      541 ccagcactgt ccttatctta ttttggggat gccacagtgt cgggacgaag ccgcgagagc
      601 gtcatcgtgt atggagtgtg tggttgtgtt cagtgtggaa acagattaaa ctggaacttg
      661 tgaccggagg gggagaatcc ccctcaggac ctccccctga tccacgatag cctgcaggca
      721 gggttgcatg gagttaggat cgacaaagga ttccagcact ggccctatcg catttgggga
      781 tcgcgcaagc gtgcagggcg gagccctgag aagtacaatc gtgggtggag tgcggtttgg
      841 ttagttatcg attaggattc actggactct ctcatgggta agaggaaaat ctcaatcctt
      901 cgggtcccgg ggcgagaggc cttcttcacc gacccatccc attcaaggca ataacctgat
      961 gacagagctg catcggaaca tctggaataa gtgcaggccg cctggattag gaatgagatc
     1021 gggctagtga atggatatgc aacggataag tcaacgataa tcgggactct tcattacggc
     1081 agcggatgat taatcatttt atcgcatgat actgcgatat tcagaatacg agttaccctt
     1141 gtgcattaag aatgacgcta tcacttcacc aaactctttg acgttacttt cataattttt
     1201 tgatgcatcg gccccccagg gggaaatata ttgatcgtga tacccaaaat cgggaatgta
     1261 tatctcattc gagaacggat ttgccgccat cttctcaatt gccgacatct tgcttatttc
     1321 actcagtcgg ggtgtcgatg accttgatga aatcatttcc gcatagacag ggactgctga
     1381 aaaagtcaaa aatgatatga ggaaaaatat tgatccgata attccaggtt tagagagatt
     1441 catgtaggtt tgtatataat atatttatat atacaccttt tcacaggatt attcccctga
     1501 agttgtcaga tatatggcgc gataaacagc acgtgtctca aatattttaa tccggcgtta
     1561 aaagaaatgg tatatacagc gcgttcagag agggtttgcg ttttgtgaat gagatatact
     1621 gcgggcagga ttgcactggg acaaattaga ttttacctgg tgtttagcac tggccctatc
     1681 gcattggggg gtcgcgcaag cgtgcggggc ggagcccctg gagcgtcatc gtgtatggag
     1741 tgcggttttc gcgtaggggg gaggggacaa tccccctcct caaacccctc cccagataca
     1801 cgatagtctg caagaagagc aaactggaaa acctctgatt ctggtttatt cccagcacta
     1861 gccctatcgc atttggggat tgcgcaaggg tgcagggcgg agcctcggga acatcggagg
     1921 ggagggcggc atgtatatta cctcacaaac cccgcaactg gtcacccctg atttcgggat
     1981 caggatacac ccatttacag aatatgcccc ctccgtcaat aggggtgcat gcatagataa
     2041 ggccgcgagt ttggcgcacg gatatcaagg cgccaagtgt tccaggctgt tccatcaatg
     2101 attttatgcc agatagagaa ttgatgatat ttgtatccgt tgatgccggg ttttgtaatg
     2161 atgtcaattt ctcatttaat tgtggcgata tttttgttga agaaaaacct gccccataac
     2221 cacttatatg cccgaatccg acaaaggcgc aaatgaggaa gatagatccg attcccttga
     2281 gtgtatatgg attcattata acccgtaata tttattcaac cctataaaac catttggaat
     2341 ggccatatcc tcacaagaca tatttgtaaa gattagaagc gtatcgcagg cagttgtatt
     2401 ttagaggaaa tgaatatact tcgaaaaaga gaacagagtt ttcatccccc gaacaactcg
     2461 cgggacctct ccagcgcctg cactgcaggg agcttctttc cggtcaaaaa ctcgatgcat
     2521 gccccgccgc cggtggagat atgggtgaag ttccggtcta ttcccatctt ctcgaccact
     2581 gcagcagtat ggccaccgcc gataatggag aactcgacct ttgatgcggt ccgcaggagc
     2641 tcgtaggtcc cggtcgcaaa gtcgggctcc tcgaagacac ctgcagggcc gttgaagacg
     2701 acagtcccgc atttccggat ggctccggag agctgcgcga tcgagtccac tcctgcatcc
     2761 atgaccgggg cttctccagg tatggcgttg acaggatact ctgccctctt tccattctcc
     2821 ttgaccgcca cgtgcccggg gaggagaatc ctgtccttga aagaagagag gagtgcgcgg
     2881 gccctcgcaa tctcgccctc gtacttgagc tgctggatca gctgggtgga gggcctgccg
     2941 atatcgaagc cggaagccga gaggaatatg ttggccacca ctccggtcac gatgatcaag
     3001 tctgcggtat ttcctttcag catgtgctcc gcgacagaga tcgagtcatc cacctttgtc
     3061 ccgccaagca caaggcacac gggacgggga gcgttctgaa aaaccctcgt gagagtggaa
     3121 acttcctttt ccatcaggag tcctgcaacc gaccgcatga ccatcgggag ccccaccatc
     3181 gtgggctgtg aacggtgagc ggtgccgaag gcgtcgttta caaaaaagtc ccccatctgg
     3241 gcgagcttct tcacgaggta taacttctgt gcatcttcgg ctttgactgt caggttctcc
     3301 tcggcgttga acctgacgtt ttcgaggacg aggacatcgc cattcttcat gctccggacc
     3361 gcatcgcggg cgcactgtcc aaagatgtcg tcaacgtact ttactctcct ctggaggagc
     3421 gcctccagcc tctcggcatg agcttccagg gtggtgaagt ccttctttcc cggccggctc
     3481 tggtgagtga caatcactac cctgctctgg gagagggcct ggattgtagg gaggtgctcg
     3541 cggaaacgct tgtcatcaag gatgtgcctg gtggtggggt cgatggggga gttgaggtcc
     3601 aaccggagaa gtatggtctt tccctccggt tttatctctt tcaaggtccc gatgggcacc
     3661 ttctttccct ccccctacac gttccttgcc ttgatcttct tcagacggaa gagttcttcc
     3721 cgctccatct cgtcaaggcg catcttgatg aagtccctgg cctcggtcag ttcggggatc
     3781 actttgaact cgagggcatt gaccctccgt ttcgtagact caatctcgtc caggagcttc
     3841 ttcatcgtgg tctcgatctc cgcactccgg atgatcgact ccaccagctc ctcgaacgag
     3901 agcgccgtct cgtcaatgac ggaggttgtc ccgagaattc cgtaaccccg ctcgtttagg
     3961 cccttccgca ccttcgaaga ctcgatctcc gggacgacga cacccatgat gttcttgctg
     4021 gccactttga tgtcaggcac ctcctggaca gagaacgccg ccgctttcac cccgatcgct
     4081 ccttcgacgg tatttgcaac cgccatcatc gactgggcgt tctcgtaccg ttcgttgaga
     4141 tcaccccgtg tgtcctttgc ctgatcgagc accttgaaga actccaggat aagcccgtcc
     4201 ctcttcatct tgaggatctt gtaccctctc tctgagaggg caatcctctt cttgaggttg
     4261 atcagctcgg atctggtggg cttgatgtct cggagtgcca tggtccctta cccctcagcc
     4321 tttccggcct gcgcgaactt ggggtggtac ttgttgatca ggtcgcggtc gatacgcacc
     4381 agctgctcga caggcagtga cgacaggagg ttccagccaa tggtgagggt atcctcgata
     4441 gtccggtcct cactgtatcc ctgacgcacg aaccggccct cgaagatgtc tgcaaactcg
     4501 aggaacatcc ggtcccgctc ggagagtgca tccttcccga cgatcgccac caggccccgg
     4561 aggtcgttgc cctcggcgta ggcggcgtac atctggtcgg atactttctt gtgatcctcg
     4621 cgggtgtgcc cttttccaat cccgaggttc atcagccggg agagggatgg cagcacgttg
     4681 atgggcgggt agatgccctt ccggtgcagc tccctgctca ccacgatctg gccctcggtt
     4741 atgtaccctg taaggtcggg gattgggtgc gtgatatcgt cacctggcat ggtgaggatc
     4801 gggaactggg tgatggagcc ctttttacct ttcacaatcc ccgcacgctc gtagatgccg
     4861 gcaaggtctg tatacatgta tccgggatag cctcgtcggc ctggcacctc ttcgcgggca
     4921 gcaccgatct gccggagcgc ctcgcagtag ttggtcatgt cggtcaggat gaccaggacg
     4981 tgcatgccaa gatcaaacgc gaggtactcg gcggtcgtca gtgcaagccg cggagtgatg
     5041 atacgctcga ctgcagggtc gtctgcaaga ttgaggaaga cgacggcacg ctcgagtgct
     5101 cccgtcctct cgaagtcctg catgaagtag ttggcctcct ccttggtgat tcccattgcg
     5161 gcaaagacca cggcgaaggc ctctttcgag ccgggaacct ttgactgcct ggcaatctgg
     5221 agagcgatct ggttgtgagg aagtcctgca ccagagaaga tcggaagctt ctgccccctg
     5281 acaagggtat ttgtaccgtc gatcgtggag ataccggtct ggataaagtc gttcggggag
     5341 cccctggcgt agggattgat tgcggcgccg gtgatgtcca ggcgtttctc cggtacgatc
     5401 tcgggtccgc catcgattgg ttttcccccc ccggagagga tgcggcccag catgtcacgc
     5461 ccgaccggca tcttgatcgt ctcaccagtg aagcggatcc cgctgtcctt tcctatcccc
     5521 gccgtggtct cgaagacctg taccaccacc agctcgtcgc tggtgtcaag tacctgtccc
     5581 ctcttgatgg tgccgtcaaa gagcacgatg ttgaccagtt cgccgtatcc taccggttcg
     5641 gtcttctcga cgaagacgag gggaccagcg atctgggtga tggttctgta ttctttcatg
     5701 ccgccgacct cagtgcgttg aattctgctt ccagttcagc ctggatcttg tcaagtgcgg
     5761 gcttgtagtc cttgatgaac ttgatctggg gcagctcgtt cttggccttg acggccagga
     5821 tctgctgcgg agtcacgccg gcgctctgtg ccgcgtacgc aaggtcggag tactggcgga
     5881 tcgccttcat catgtcgtac tgctttttca tgtcgcagaa ggtgtccacc gcatcgtagg
     5941 cgttctgctg caggaagatc tcgcggatca tcctggccac ctcgatagtg atctgctctg
     6001 cctccggaag ggcgtccgac ccgacaagct gcacgatctc ctgcagttca gcctccttct
     6061 ggaggatctc catggcccat gagcggatgg tgttccattc aggggacact tccttgtcat
     6121 accagtcatg gaggctgtca aggtacagcg agtaggagtt gagccagttg atggcaggga
     6181 agtgcctgcg ctgggagagc tttgcatcga gggcccagaa taccttcacg atacggagag
     6241 tgttctgggt gacaggctcg gagaagtctc ctccgggggg ggatacggct ccgatgacag
     6301 acaccgaccc catgcttccg gcaagggtct ttactctccc ggcccgctcg tagaactccg
     6361 agaggcgtgc tgcaagatac gcggggtacc cctcttctcc cggcatctct tcaagacgcg
     6421 aggagatctc acgcatggct tccgcccacc gggaggtgga gtccgccatc agcgagacat
     6481 cgtaccccat atccctgaag tactcggcga tggtgatccc ggtatacacc gatgcctcac
     6541 gggccgccac cggcatattg ctggtgtttg cgatgagcac agtccgctcc atcaggggct
     6601 ttcctgactt cgggtcctcg agctccggga actcggtcag cacctcggtc atctcgttgc
     6661 cgcgctcacc acacccgatg tagaccacga tctcggcatc gctccacttt gcgagctgct
     6721 gctgggtgac cgtcttcccg ctcccgaacg gcccggggat cgccgcggtc ccgccctttg
     6781 cgatgggaaa gagaccgtcg aggatacgct gcccggtgat aaggggaacc gtagggttca
     6841 tcttctcgtt gacaggccgg ggtacacgga cgggccattt ctgcatcatg gagatctggg
     6901 cgccgttatc cagggtgcag accacctcgt cgacagtgaa catgccggcc ttgatggtct
     6961 ttatggttcc gcccttcaca ttgggcggga tcatgacccg gtggacgatg ttcgtctcct
     7021 ggacttcgcc gataatgctc ccaggggcaa cggttgcgcc ttcttttgcc agcggtttga
     7081 actcccactt cttcgcatga tcaagacccg gggccgaaac cccgcgctgg atgaagtcgc
     7141 ccatcttgtc tacgaggaca gagaggggtc tctggatgcc gtcatagata ctggtcagaa
     7201 gcccaggccc aagctcgact gcgagcgaaa gcccggtgtt gatcaccggt tcgccgggcc
     7261 tgatccccga agtatcctcg tacacctgga tgatgatgtt ctcaccctgt atcttgatca
     7321 cttctcccat gagttcttcg ttgccgacct tcaccacgtc gtacatgtgg gcgtcaatcc
     7381 ccacggcggt gaccacagga ccggcgattc tcttgagaac tcctgttgta tcactttttg
     7441 gtttctctgt cttcacttcc acaggtcaac acccactgaa cgcttaattc tctccctcat
     7501 ggagagtcct ccttcctctg ccccgatggt gattaccgtc ggttttaccg attcctcaag
     7561 tgtcatctgg agacggcggg ggatcttctt caggtcactg ctctggagca ccaggatccc
     7621 cacatcagaa tcatcaagaa cccggttgat atgtgccagg agcatctcgt cgttctcgac
     7681 tggatacgtt tttcgtatcc cggcgagcct gaatccaagg atgaactcct ggttcccaat
     7741 cactgcgatc tccattatat caccaggtac cccctgatcc ggtcaggagt gaggttcgat
     7801 tcctttccgc gtgccagggc tctgagattg aatatctcgt acttcttcat ctccagatag
     7861 gcgaggatgg gatggatgga gaacggatgc agcttggaca tcgactccat ctctgcgagc
     7921 tggactttga tgagatcgat ctcaatctcg cggattgact tctcctcgcg gagcttctca
     7981 aggagttcgc gtaatactct cactttcacg agtggcacaa gtgcgtcgat gaactcattc
     8041 cggtcacgga tgtacatcag cctctggatt tgctggggtg agatagtgcc gccgggaatg
     8101 attgctgcca tcatctcctc accggactga tcggcacgga aacggaacat gttgctgatg
     8161 ttcgtgatgt caatatccag ggtgatatag tccatgaact gcttgccccc cttgatgccg
     8221 ctcatggcca ttgagacgag ttcagcgtaa aactgcttgt agagctcgtt ctcgaccctg
     8281 ggcagaaact cctcttttac ccctccctca aacgaccttg aaagaagcgg gtatacccct
     8341 tttcctttca gggcctcgat gatccgctcg ggcgttggtt cctcgatgag cctgtcaaga
     8401 aagtgcttgt caagctcgcc agcgggtatc agcacctccc tgatcttgcc ggacctctcc
     8461 ccgtgaagct tgccacggag tacggtgagc acgttctgga tgtcccactt tcgcaggtat
     8521 gcccgtgtga ggtctttcag tgttccagga gtgattttct ggatgttctg atactccttc
     8581 gccaggttcc agctcagtgc gatctcgatg aggtcaatgc cgtggaacga ggtggcaagt
     8641 tcgtcgatct ctttcttgta ctgggtctcc tcgataaacc tcgttatctc cgagagaccc
     8701 atgttcagca tccgtaagta ctcttccctg gggaggagct tggctttccg cacccgcatc
     8761 ctggtgcaca cgtagatgta gggaactggt cctgctctca cttctgccat ccgagcctcc
     8821 ttctacccaa atagcaagtc tgacgcatct ttgagtcctg attcccatat catgtccagg
     8881 aaagtccgat aactgtaatc aagctgcaga gtgccatcca tgctctcaac aatcaccccg
     8941 ccatctatct ccgcatcctt gcccgttgaa aatgctttaa attcaggttt ttccgcaagt
     9001 atccgttcaa ggagcgcctt atccctctgg tttacccgca caataccccc gggtatctgt
     9061 tgttttgcct ccgtgaggag gcgggtgatg gcgtcccggt ggaaactttc gggcaacccc
     9121 gcaattgtga cgatggctct ctcatatacc tggtcaagga gatccttctg ggtgttcaga
     9181 agttccctct tgaccaggag gttggcagcc gagatctcct gtgagaggat ataccctgac
     9241 tgacgttcag catcatcgtt tgcggcctgc ttgatcttcg ccgcacgctc ctgggcagcg
     9301 gtgagaaccc tggaagtctc ctcctggctc tcactcctga tcctctgcat ttcctgctgg
     9361 cctttctcgc ggatatctgc aaggactgcc tccaatccca taatcggctc cctagaacaa
     9421 taacaggagg ccgaccacga gaccgaagat cacaatggtc tctggaataa cggttaacag
     9481 aagcaccagt ccaaagacgt ccttgttctc tgccgtggcg cccacggccg ccgatccaat
     9541 agccatctcg gcaagcccgg tccctatacc ggtaagacct accgcgatac ctgcgcccag
     9601 tgctttcatg ccaagctgtg atgccttgac catttccacg gtcatttctg ttgctgcgac
     9661 tgctgttgct gcgtctgcca taatttaatc ctccataaat ctctttttca tcccgaatgg
     9721 ggtgtatttt ttgcctccac ctttgtagaa cttggtgaag aattcgacat actgcaacct
     9781 gattgactgg agcccgccac caagcatccc aagaatagta ttcagaagat gccctatcac
     9841 gaaaaccagt gcgccaacga tgatgataat aaccccaact gcgctcaaag actcgagttg
     9901 gggttcaata atcaggccga tggcaataaa gttcaccacc attgcaattg ccacagagga
     9961 aagacccacg ccgaccagac gggcatagga aagcacgtga ctcagaatgg tgggcaattc
    10021 cacgatttca agggcggaat cccggataat gaagatgaca ccgagaagaa tcagtacgat
    10081 cccgaggatg gacgagaggt tcagtcccat cacgattggt ggcaatccag tgaaatccgg
    10141 catgaacggc atggtgaatg atgcccagag catcagcagg attccccaga gaaccatgat
    10201 ccatccaagg ttggccatga cagcctttac acggtgggac ccatggtcct gccttgcgtg
    10261 gttgataatc ccgaggaccc tcccaagtgt gatctgcagg atcccaatcc agatggccat
    10321 gatcatcagg gaagtgactt cagggccatg tccggccgca tgtcctccga tgttcaggtg
    10381 cctggagaac aggatgggcg accacgggag ggaaaacccg aaaaactcgc tgaaaaggat
    10441 tccaaatacg atgctcgaga tgcttgcatt ccggaggact ttcaggagtc ttgtcccgtc
    10501 atctcctttc atgaacctct ggaggccgat gctcagtgca agaaggattg caccatagcc
    10561 cacgtcaccg agaatgatcc cgaagaaaat cgggaatatg atcgatacca gcagggtagg
    10621 gtcgatctca gtatacctgg gcctcgagta cacgtccatt agcatctcgg ttggtttcgc
    10681 gaatccgacg ttgtggtact cagtgggggc gatgtcctcg atctccgaac cttccagctc
    10741 gtacacgtag accttgccgc cggtagcata gtgaatcccc tgtcggaact tctccagttc
    10801 cgcagtggga acccagccct cgactatgaa cgtctcaccg gtggttgcaa agcgcagcgg
    10861 tgcttcagcc atctccacat ctgcggtgag aagctcgtta caggccagga aaaggttctt
    10921 gtgctccttc ttgaggtcct cgagccgtct gttgatgtcc tcgagttcac ctttcactgc
    10981 caggatctga ctctcgtaga agccgatgcg atctttggcg agaccgttct cttccggtat
    11041 gggaactgcc tggaactgtg catcgagaag tgcctgttcc gcgcttccac ggtgctggtt
    11101 tgaaactata aggatgacaa attttgcctt ttttgtatca ctggtgaaca tttcgtgggg
    11161 tatatcaagg gatatgtccc gcaagatgta gccagagaat acagagaatg tcttgtaacc
    11221 ccggagaagc tccagatcga agggcacatc cctgaagggc tgtaatccgc tgatcttctg
    11281 ctcatactcc ttcacctggg tatccaaccg tgatctccgt cccaggagtt cttcgacctc
    11341 ctgttccagg attggtaatt ccttctcaac tacctctttc agatgtgcat ttttctgtct
    11401 ttcgctttgg gatgcctcct gatcgtcacg gatcatgaac gcattggtga tgcccctgag
    11461 ccgaagcagt tcgcgggaag tctcatttgc ctgagggagg ggggtaccta tccgataccc
    11521 ctcgtactct atcctgtctt tttccacgaa ctcttcaata tggaacaggt gctgccggta
    11581 catctcccgg atcacaggtt ccatctggga tttggtggcc gcaatcagga cacgggtcat
    11641 tccctcagga gtgagcatgg agccgctcct tgaatcgcga caccagaagg tccgctgccc
    11701 gcccgagatt gcgtgttcca cgctccttca gctcggcagc acgcctcttt ccttccgcaa
    11761 ggatctcgtc atgttttttt gcggcgttct gccgggcctc tgcgagcttg gccttcttgt
    11821 actcctcagc atcatgggtt gctttcatga tcaggttgtc tgcttcgagc tcggcgtttg
    11881 cactgttccg cttcttctcg gccagagcat cgctgatcat cttctggtac tctgattctg
    11941 tcgctttgat gttcttcaaa acctcagtct tcatccaacc ctcctctcac ggcactgaaa
    12001 tgttagaccg gaattgttct tatatctgtt gatatttttt cgggtgaaca gaagtgatat
    12061 aaaaggattg gggggaatcc gggttcaata ggctgatata ttggtaagaa tgggctctcc
    12121 aaaggagagt atattggccg ggggtgacca ttcctgaaag gaaggatata accaaaatta
    12181 ccggctctgt tccagaatat ggtcaccgca gggaaggtac ggggacaact cccagatcca
    12241 ctccatccgg aataccgctg accagtatcc cggaggcggt gccgagcagg cgaattcctc
    12301 ccatctcttc agaggaacag agaaggtgct gctcaggatg cgttcccctc tcgatcgtgc
    12361 acgagaggag tatctcatct cccgccgata cgacgtagcg gaggcggcgg gaaccgggat
    12421 ggctgcgggg catcaccacg agaggaaggc ggtacgttct caccagctca atgagcatcc
    12481 atgcagcctg aaccgatcca ccctcgggag cagataccgc gacaatgtgc gatggctcaa
    12541 gggtctggca gtactcttca tcgtgcgttt ctatgcagag cggaaaacgg gaccctgcat
    12601 ttcctgcaag gaggaacgat gatttactcc tgaccagcag gagagtgtct ttcccgattt
    12661 gaaccatttt ctcctgcatg cgtgtggata cctcctatat gaacaggttg aagatcacca
    12721 gtgcgatgac catcatgatg catgagtgct tgattccggc cttgatggaa ccctccccca
    12781 tctgccctgc gatgaggccc gagaggagcg cctgcaggac gaccgagtgg aacatcagcc
    12841 gcaggatggc catgatcgat gctgcactga cgttcgatat ggcggttccc tccggtattc
    12901 cccctgcatc tacactgcca aggattggaa tgaagttgtt cgagatgact gcgacgacaa
    12961 agacgaatac cacgaacgca aggtagatga tcgcggtgta tatgaacatc tctgagagcc
    13021 gttcccgttt cagaagatcg gacatcttgg cgtcgcttgc cgcgatggcg agcacgtccc
    13081 cgatagatcc gctcatctgg ctggccttgg tgataagggt aaccgtgcgg gcgatcaggg
    13141 gtgtccggat gcggtcctcg aagcggatga gcgcatctgt gaaactcgct ccccagtcga
    13201 tatcccgctt gatccgcttg atctcgtagg agaggagacc gaggttggtg ttcaccatga
    13261 tccctatggc ctgggcgatt gtcaggccta cctggttaat ccctgccatc ctgtccagga
    13321 actcagggat gagggattcc atgccctgga cgttccggcg ccacatctcg tagaagaatg
    13381 cgtaggggac catgacaatg agcagtgcaa tcatcacgtg gtcgtccacg acgtcgatgt
    13441 agacctcgag atcctggtac tggggcacca cccagaatac ggcaaccacg taaatgattg
    13501 caagggggac ggtgatgaag agcgtgagat tgtagttctt ctggaaggcg tcgaagggat
    13561 ggcggaagaa ctcgcgtgca ttcctcaccc tgtcataatg gacgagccgg gcaaagaacg
    13621 gctcctcccc ctccctgtgg tagaccctga catcactgta ctcgtgcaac catttcgcac
    13681 ggacatatcg ctcgacggtc tcacccttga gggagatgat atcgatgagg aggatgaaga
    13741 ccagggaccc gatgggaaac acgaggtaga tcaccgcggt gagctggagg atggccaccc
    13801 cgcccatcat ccccatcacc accatgatga tgatgaggaa gagcggcccg gcgacgaaga
    13861 gcgtgacata cgactccgca acaagggaga ggaagctgag gaactgcttc tgttcgaacc
    13921 tcgcctcttc ctggtagagc ctgacacgtc ctgacaggaa gctcacgagg tccccg
//
LOCUS       DAOQYR010000060        13683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_4980_length_13683_cov_9.544541, whole genome shotgun sequence.
ACCESSION   DAOQYR010000060 DAOQYR010000000
VERSION     DAOQYR010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 13683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13683
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4980_length_13683_cov_9.544541"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /gene="frhB"
                     /locus_tag="PKM87_07945"
     CDS             complement(<1..707)
                     /gene="frhB"
                     /locus_tag="PKM87_07945"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit beta"
                     /protein_id="HNQ30037.1"
                     /translation="MAGELGKYKLCVSARSADKAILRKAQDGGIVTQLFKFALEEGII
                     DGAIVAGPGDEPWRPRPFIATTVEELMTSSGTKYNISPQVSWIKEATRSYGLDKIGIV
                     GTPCQMQAVRKGQLYPVGLRDVPDKIKLAIGIFCMENFSYQSVKQLVEDHAAMKMEAV
                     KKMEIGKGKFWVYGQRGQVVQLPLKVTHKYEQPGCHVCLDYVANLADVSTGSVGSPDG
                     WSTVFVRTRNGDDIWSKA"
     gene            complement(718..1479)
                     /gene="frhG"
                     /locus_tag="PKM87_07950"
     CDS             complement(718..1479)
                     /gene="frhG"
                     /locus_tag="PKM87_07950"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit gamma"
                     /protein_id="HNQ30038.1"
                     /translation="MADKITVGHVHMSGCTGCLVSLADNYGGLLTILDKYADLVYGLT
                     LADVRHIPKMDVALVEGSVCIQDKISVQEIKETREKAAIVVAVGGCACYGNITRFGRG
                     GQPNQPQHESYLPVGDLIKVDVYIPGCAPCPQLIRNVCVMAYLLLKGNKDQQELAKKY
                     LTPLMQLAERGTEACGCDLMYDVVNQGLCMGCGSCAAACPVRAITHEYGKPNVNREMC
                     IKCGACYAQCPRSFFNFDVVPEFEGIMDAIDAALK"
     gene            complement(1523..2059)
                     /gene="frhD"
                     /locus_tag="PKM87_07955"
     CDS             complement(1523..2059)
                     /gene="frhD"
                     /locus_tag="PKM87_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-reducing hydrogenase, FrhD
                     protein"
                     /protein_id="HNQ30039.1"
                     /translation="MLYPEIVIAGCGNPLFADDGFGPAVVEELLRLDLPDNIKAVDAG
                     LGGPHFIFTLLDTEVTRKLIIVDTVDFGGNPGELVRLKVEDLPPGSYRDAHSWDLTEP
                     LHRLKDSMEIAVIGCQPKHVSAPEFEMGLSDEVSGAIPRAVREVLALIGVDYGTTLEN
                     LRKEERQPEAGGEACGES"
     gene            complement(2070..3437)
                     /gene="frhA"
                     /locus_tag="PKM87_07960"
     CDS             complement(2070..3437)
                     /gene="frhA"
                     /locus_tag="PKM87_07960"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit alpha"
                     /protein_id="HNQ30040.1"
                     /translation="MSKIVEISPTTRHEGHSKLVLKVDDAGIIERGDWLSITPVRGVE
                     KLALGKTMDQVPKIASRVCGICPIAHTLAGIESMEASIRCEIPRDAKLLRIILQCANR
                     LHSHALHNILTLPDMYLPGTDTKINPFSPQEPVRTVAKRIQRLREIGQTIGEIAGGEA
                     VHPSNPRVGGMYKACTQHAKTKMYDLAKEGLVLAHEQMDFMIAILRNYQKRDWAEVGG
                     KQIPIPKTLGYHNQGYMAADPMYGSSSLDECPGWNPDRWTDVRPWDWYMGEGEVSLAD
                     PSYPIGGTSPVGTKVNPQMEACTGVPLYDGSPVEVGPRARLVQFKNYDEKGTIGQQVA
                     RQMEYPDCLYTIINALDDYNPNGKVLADYIPQGDGSLGWAANEAPRGCDVHLAKVKDG
                     RVLYYEMLVPTTWNFPTCSRALTGAPWQLAEVIVRGYDPCVSCATHMIVIDEDKRLVA
                     QKLIQ"
     gene            complement(3892..4827)
                     /gene="mptA"
                     /locus_tag="PKM87_07965"
     CDS             complement(3892..4827)
                     /gene="mptA"
                     /locus_tag="PKM87_07965"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HNQ30041.1"
                     /translation="MPDIQSTTPDVRINLTRVGVKNVKKLVEVSRPDKRPVIFISNFD
                     VFVDLPGSLKGANLSRNFEVIDEVLQQAIDGDVKEIENLCSVVARKLLDRHEYADRTE
                     VLMNSQFMVKRETPVSHTSCHEVVKVHARAVARRTFRDPIVRKSIGAEVTGMTACPCA
                     QDIMKERAISVLQNLEIPKEKIDAFLAEVPMATHNQRGRGVLCIEIDDDQHVKLEKII
                     RILKESMSSSIFELLKRGDESYVVLSAHKNPRFVEDCVREIAKKVLAEFKDLPGDSLI
                     TIMQTNEESIHQHDAYAERQATMAELYVELNGEHS"
     gene            4951..5943
                     /gene="ilvC"
                     /locus_tag="PKM87_07970"
     CDS             4951..5943
                     /gene="ilvC"
                     /locus_tag="PKM87_07970"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNQ30042.1"
                     /translation="MMEKYYDTDADLGVLKGKRIAVIGYGSQGRGQALNLRDSGLDVV
                     IGLRPGRSWQAASADGFKVYPVAEAVKSAQVVQVLVPDEHQGAIYQAEIRPHLSENKC
                     LMFSHGFNIHFGQIVPPPDIDVIMVAPKGPGHMVRRTYEEGKGVPALIAIHQNYTGNA
                     QQIALAYAKGIGATRAVVLETTFREETETDLFGEQVVLCGGITSLIKAGFETLVDAGY
                     APEMAYLEVLHETKLIVDLIYEGGFTKMRNSISNTAQYGDLTRGPRVIGPESYLAMQE
                     ILKEIQSGEFAREWMLENLVNRPVFNALTRADEEHLVEQVGKEVRAMMPQFKKQ"
     gene            6346..6963
                     /locus_tag="PKM87_07975"
     CDS             6346..6963
                     /locus_tag="PKM87_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HNQ30043.1"
                     /translation="MVEPYETLLKKAYSHVTEPTESVERFTVPEARSYIEGKTTVLEN
                     FSEIADTVRRDKDHLMKFLLGELGTAGKIDGTRAIFNGKFEAALINSLVKGYVDDYVI
                     CSECGKPDTRLVKDDRVLLLRCDACGGHRPVRKRKARTEPVTNEISEGAILDLEVQSV
                     SRRGDGVVRMGRYILYIPGGKPGQKVKVRITRVSGSIVFTERVLV"
     gene            complement(7032..7307)
                     /locus_tag="PKM87_07980"
     CDS             complement(7032..7307)
                     /locus_tag="PKM87_07980"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit L"
                     /protein_id="HNQ30044.1"
                     /translation="MNIKILELEKDKAKLLIQGEGHTFMNVLTEEILKDPDVDVAKYL
                     IKFQFSDPELLVTTNGKKAPLDAIREACQRISGSCDELLEILSKGRS"
     gene            complement(7391..8110)
                     /locus_tag="PKM87_07985"
     CDS             complement(7391..8110)
                     /locus_tag="PKM87_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ30045.1"
                     /translation="MQIVLLGTGDAIGTPRIGCSCTQCTHAHEHGLQRLRTSLLVERQ
                     GRHILIDSGPDLRQQLLAAGSPHIDAVLWTHGHYDHFMGFAEFYRVQDVPDVYAPLPV
                     MDYCGRIFQFLLRDRHPVRAYCPFTLFGVECTFLTVDHPPTYTCGVLLEADGVRIGYT
                     SDTTLRLPEETSKHLEGTDLLFVDALLPSHLHVPKHMNYKEACDLARTLGAGDFRCVH
                     QSHHLPWGLPHLGCDGETFTF"
     gene            complement(8112..9389)
                     /locus_tag="PKM87_07990"
     CDS             complement(8112..9389)
                     /locus_tag="PKM87_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ30046.1"
                     /translation="MAADDETLKLIEILLTAELFNSHQNLDANDLTPECRNLFTVNGT
                     AEVKRPLHVSEGMVRRSLGIENACRKLEQAPVVTCEEFGQRIKLTALEPAARWFLKIG
                     GMPQIEKNPTLAFFFEHFESTKVSYEKARQDNPPIEDTRVFLQSRLTKILADDERLKN
                     AMDLIILSAPEEVEQDFNDLVCTRAQENIIRKIQVARTHRDILRRHRIHEVGKLLFVG
                     PPGTGKTSLALALARKLHLPLLEVRLSMITSQYLGETSKNIDRIFELAKALSPCILFI
                     DEFDFVAKSRVADDHGAMKRAVNSLLKNIDQVSLIRHGVLLVGATNHPQLLDEAAWRR
                     FDEVVEFGLPDREMRRAILEKVSTGMQYMFDIPDLAERTEGFSGADLRIMIKEALLSS
                     LMDHRDAINITDIERGITVVKNRDTIRSQNWLS"
     gene            complement(9434..10309)
                     /gene="map"
                     /locus_tag="PKM87_07995"
     CDS             complement(9434..10309)
                     /gene="map"
                     /locus_tag="PKM87_07995"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II methionyl aminopeptidase"
                     /protein_id="HNQ30047.1"
                     /translation="MKDEVMDKYIEAGKIASEVRRKGAGEIRVGASYLAVVDTIEEMV
                     RDAGAGLAFPLNVSLNEDAAHDTASAGDERVFAQGDVVKLDLGVHVDGYIADTAMTVD
                     LGDHPLLVEASARGLASAISMVAPGVTVGELGSAIHEEIESRGFRPVANLTGHGLDRY
                     RIHTPPNIPNIRLNGGAVLEEGMVFAIEPFASTGIGQVSEKHRTEIYQQLAVKPVRLP
                     SARRVLEEIRQRQGMPFSRRWLNEGKLDLALSTLVRSQVIRAYPVLSDVPGSLVSQAE
                     HTLIVTSEGCLVTTA"
     gene            complement(10321..11448)
                     /locus_tag="PKM87_08000"
     CDS             complement(10321..11448)
                     /locus_tag="PKM87_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNQ30048.1"
                     /translation="MDALEEAILRSGSTAYVAYGSSQDADMRYLTRFSTTDPIVYIRK
                     PGEKGTVVVSQMEYERAEREATAAVISRAQAGLFEILKGEQDRWRALARMIAGLVQGK
                     VLIPPQFPYALGRELSELVAVEVDREALARMRAAKSPWEIGRISAVQAATEEAMDRGI
                     QVIRKARVKGGLLYMGAVPLTSGMVRAAMHQVLLKRGCIPTDTIVSCGEESALPHLRG
                     EGALKADEPIVIDIFPRDEQTGYYSDMSRTVCSGEPSPDVLEMFQAVRDAQDLAAETI
                     RPGVTGEEVYRAVVGFFEETGYGTGSSGFVHNLGHGVGLEVHEAPSLGPSGEVLLPGN
                     VVTNEPGLYMPGIGGVRLENIGVIGPGGFATLTRYPRDLAV"
     gene            11510..11767
                     /locus_tag="PKM87_08005"
     CDS             11510..11767
                     /locus_tag="PKM87_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30049.1"
                     /translation="MDEATKEQLKWKFYRLAIILNAIVLLVALGVIAILKLPEPVALP
                     GGIALILLAVGLAIYFRKQYVSTKKWLDEQASKDRAGGHGQ"
     gene            11764..12054
                     /locus_tag="PKM87_08010"
     CDS             11764..12054
                     /locus_tag="PKM87_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30050.1"
                     /translation="MIEKVKGEIELMQRHIQVLRTVVAHQPIGIMKLSELMDLPFHRI
                     RYSLRVLEQMGYIRASQSGAVATPQSVELLAGLDQELGEMIRLLESMRHQGG"
     gene            12152..12224
                     /locus_tag="PKM87_08015"
     tRNA            12152..12224
                     /locus_tag="PKM87_08015"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12185..12187,aa:Asn,seq:gtt)
     gene            12238..12312
                     /locus_tag="PKM87_08020"
     tRNA            12238..12312
                     /locus_tag="PKM87_08020"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12273..12275,aa:Ile,seq:cat)
     gene            12396..13424
                     /locus_tag="PKM87_08025"
     CDS             12396..13424
                     /locus_tag="PKM87_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30051.1"
                     /translation="MKCPVCGDGCIQDAPAVLESLASVFSRCPRCTFRVLDKSAPLKG
                     RYYGTPCECGKRFIDEVFAHLYVILVEEGIFSGSEPLRAVGSPLLHPGFFIAGPPFLP
                     PGSLVLLSPLVTESAARRMITEVPEVRGVVRSGDFVPGATDPSLATVPRSYELLAGCD
                     VRAGIFPTGAGPLVIYTQQSQMHIEFPRLENPKIQSVERHIAAQRPTWCVDACSGAGT
                     LGLCAARHGIPGVILNDAWYSAAFWSATNLHVNRMFFQVEGVRIHLSFREMSMHPVGR
                     DPVKVAEASGGQSIAVYHGDFRQLWKVLPREPVCAVLDLFEKSDTRMTEAAMKEWQSR
                     VPGEVFIP"
     gene            13439..>13683
                     /gene="ffs"
                     /locus_tag="PKM87_08030"
     ncRNA           13439..>13683
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKM87_08030"
                     /product="signal recognition particle sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01857"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal-type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01857"
BASE COUNT         2656 a         3959 c         3988 g         3080 t
ORIGIN      
        1 gccttggacc agatgtcgtc gccgttgcgg gtccggacaa agaccgtgct ccagccatcg
       61 gggcttccaa cggacccggt cgagacatcc gcaaggttcg cgacgtagtc aaggcagacg
      121 tggcagccgg gctgctcgta cttgtgggtc accttgaggg gaagctggac gacctgcccg
      181 cgctgcccgt agacccagaa cttgcccttc ccgatctcca tcttcttgac tgcttccatc
      241 ttcatggctg catggtcctc gacgagctgt ttcacgctct ggtacgagaa gttctccatg
      301 cagaagatgc cgatggcgag cttgatcttg tcgggaacgt cccggagccc cactgggtag
      361 agctggccct tcctgaccgc ctgcatctgg cacggtgtcc ccacgatacc gatcttgtca
      421 agaccgtagc tccgggtggc ctccttgatc caggacacct gggggctgat gttgtacttg
      481 gtcccgctcg aggtcatcag ctcctcgacg gtggttgcaa tgaacgggcg gggcctccag
      541 ggctcgtctc ccggtcctgc tacgatcgcg ccatcgatga ttccctcttc aagggcgaac
      601 ttgaagagct gggtgacaat acccccatcc tgggccttcc ggagaatggc cttgtcagcg
      661 ctgcgtgccg atacacagag tttgtatttt ccgagttctc ctgccatctc tctcacctca
      721 cttgagtgcg gcatcaatgg catccatgat gccctcgaat tcggggacca cgtcaaagtt
      781 gaagaagctc ctcgggcact gggcgtagca tgcaccgcac ttgatgcaca tctccctgtt
      841 cacgttgggc ttcccgtact cgtgggtaat ggccctgacc gggcatgccg ctgcgcacga
      901 tccgcagccc atgcagaggc cctggtttac cacatcgtac atcaggtcgc agccgcaggc
      961 ttcggtcccg cgctcggcaa gctgcatcag cggggtcaag tatttcttcg ccagctcctg
     1021 ctggtccttg ttgcctttca ggagcaggta cgccatcacg cacacgttcc tgatcaactg
     1081 cgggcagggg gcgcatccgg ggatgtagac atccaccttg atcaggtccc ccaccggcag
     1141 ataggactcg tgctggggct ggttgggctg gccaccgcgg ccgaaccggg tgatgttgcc
     1201 atagcaggca cagccgccta ctgccaccac aatcgctgcc ttctcgcggg tctctttgat
     1261 ctcctgtact gagatcttgt cctggataca caccgacccc tccaccagtg ccacgtccat
     1321 cttggggatg tgccgcacgt cggcaagggt caggccatag acaaggtcgg cgtatttgtc
     1381 aagtatggtt aacaatccac catagttgtc tgctaaggat accagacagc ccgtgcatcc
     1441 gctcatgtgc acgtggccaa ctgtaatctt gtctgccaca ggcttttcct cctttttaat
     1501 cgtttcagcc tttttctcca cgtcagcttt caccgcaggc ttcgcctccg gcttcgggct
     1561 ggcgctcttc tttccgaaga ttctcgagag tagtcccata atccactccg atcagggcaa
     1621 gcacttcacg tactgccctg ggtatcgcgc ctgaaacctc atcagagagc cccatctcga
     1681 attcaggggc gctcacatgc ttgggctggc atccaatgac tgcaatctcc atcgagtctt
     1741 tcaaccggtg gagcggctcg gtgaggtccc aggagtgggc gtcccggtaa cttcccggcg
     1801 gcaggtcttc aactttcagt cgtaccagtt caccagggtt tcccccgaag tccacggtgt
     1861 caactatgat caacttccgt gtcacttccg tatcaagcag cgtgaaaatg aaatgcgggc
     1921 ctccgaggcc tgcatccacg gctttgatat tgtcggggag atcgaggcgc agaagctcct
     1981 caaccactgc cggcccgaac ccgtcatcag cgaacaacgg gttgccacac cctgcaatca
     2041 caatctctgg atacagcatg cgagcagtct cactggatga gtttctgggc aacgagcctc
     2101 ttgtcctcgt cgatgactat catgtgggtt gcacatgaca cgcacgggtc gtatcctcgg
     2161 acgattactt ccgcgagctg ccagggtgca ccggtcaggg cgcggctgca ggtggggaag
     2221 ttccaggtgg tgggaacgag catctcgtag tagaggaccc ttccgtcctt gacctttgca
     2281 aggtggacat cgcatccgcg gggtgcctcg tttgcggccc acccaagcga tccatcaccc
     2341 tgcgggatat agtctgcaag gacctttccg ttggggttgt aatcgtcaag ggcgttgatg
     2401 atggtgtata ggcagtctgg atattccatc tggcgtgcga cctgctgccc gatggtgcct
     2461 ttctcatcgt agttcttgaa ctggacaagc cgtgcccttg gaccgacttc taccggggag
     2521 ccgtcataga gcggcacacc cgtgcaggcc tccatctggg ggttgacctt ggttccgaca
     2581 ggagaggtac caccgatagg atagcttggg tctgcaaggc tgacctcgcc ttctcccatg
     2641 taccagtccc atgggcggac atcggtccac cggtcggggt tccagcctgg acactcgtca
     2701 aggctcgagc ttccgtacat tgggtcagct gccatgtagc cctggttgtg gtatccaagg
     2761 gtcttcggga tggggatctg ttttccgccg acctctgccc agtcgcgctt ctggtagttg
     2821 cggaggatgg ctatcatgaa gtccatctgc tcgtgggcaa gaacaagtcc ctccttcgcg
     2881 aggtcgtaca tcttggtctt cgcatgctgt gtgcaggcct tgtacattcc tccgacacgg
     2941 gggttgctgg ggtgcaccgc ttcgccgccc gcgatctcgc cgatggtctg gccgatctcg
     3001 cggagcctct ggatccgctt cgcaaccgtc ctgaccggct cctgggggga gaatgggttg
     3061 atcttggtgt cggtcccggg gagatacata tccgggagtg tcaggatgtt gtggagtgca
     3121 tggctgtgga gcctattggc gcactggaga atgatcctga gcagtttggc atccctgggg
     3181 atctcgcagc ggatggaggc ctccatggac tcgattcctg cgagggtgtg ggcgatcgga
     3241 cagataccgc agacgcggga cgcgatcttc gggacctggt ccatcgtctt cccgagggcc
     3301 agcttctcta cccctctcac aggggtaatg gagagccagt caccacgctc gatgatacct
     3361 gcatcgtcca ccttcaggac gagctttgag tgtccctcat gcctcgtggt tggggagatc
     3421 tctacaattt tcgacaatat aatcgcctca tggtattctc atgttctctt tgcgataccg
     3481 gtgtaattcc acactcacct ctcatagaga gatgtttgac cgtacagagt acgttcagtg
     3541 tgaatgtaat attgtcctca tatttatcta ctgtgcagaa attctgagga agcgttcgaa
     3601 ggttaaaatg aagccctttt aacgaaaagg ctccttttat cgctttaatc actacagaat
     3661 cctgaagttg gctttgcact gaatggaaag ttttgtgaat acaaccgttt ttagccgttt
     3721 tctctctgga tttttcatgc aatccccgcg ctttcaggga ggggatgtca ggttgtggga
     3781 gagagaaact tatcccaata taaattgggc gaattaaaaa atgaaggctt taaaatcttt
     3841 tttgcagatg gtatttatga ggtcctgagg ggtggggaca gccctctcgc atcaggaatg
     3901 ctccccgttc agttccacgt acagttccgc catcgtggcc tgccgctcgg cgtaggcatc
     3961 gtgctggtgg atgctctcct cgtttgtctg catgatggtg atcagcgaat cgccaggcag
     4021 gtccttgaac tccgccagca ccttctttgc gatctcccgt acgcagtcct caacgaaacg
     4081 aggattcttg tgggcgctca gcacgacgta actctcgtca cccctcttga gcagctcgaa
     4141 gatggaggag ctcatggact ccttgaggat acggatgatc ttctctagct tgacgtgctg
     4201 gtcatcatct atctcgatgc acaggacgcc ccgcccacgc tggttgtggg tcgccatcgg
     4261 gacctcggcg agaaacgcat cgatcttctc ttttggtatc tccaggttct ggaggaccga
     4321 tatggcccgc tccttcatga tatcctgtgc acaggggcat gcagtcattc ccgtcacctc
     4381 ggccccgatg ctctttctca cgatgggatc gcggaaggtt ctcctggcaa ccgcacgggc
     4441 atgcaccttg accacctcgt ggcagctggt gtgggagaca ggagtctcgc gcttgaccat
     4501 gaactggctg ttcatcagca cctcggtgcg gtcggcatac tcgtgccggt cgaggagttt
     4561 tcgtgccacc acgctgcaga ggttctcgat ctctttgaca tcgccgtcga tggcctgctg
     4621 gagcacctcg tcgattacct caaagttccg ggagaggttg gcccccttga ggctgcccgg
     4681 aaggtcaacg aagacgtcga agttggagat gaagatcacc ggcctcttat cgggcctcga
     4741 gacctccacc agtttcttga cgttcttcac acccaccctg gtaagattga tcctgacatc
     4801 cggggtggtg gactgaatat ctggcaaatc acgtccggat ttgtgtttta cggcattggg
     4861 aatagcgcat cgctcccaag aatgatgtgg aatgtaattt ttctatataa tatttaagtg
     4921 ctctgttact gatatctgtg ggatcagcgt atgatggaga aatactatga taccgatgcg
     4981 gaccttgggg ttctgaaggg gaagcgtatt gcagtgatcg gctacgggtc gcaggggagg
     5041 gggcaggccc tcaacctccg ggacagcggc ctcgacgtcg tgatcgggct tcgacccggt
     5101 cgttcctggc aggcggcgtc tgcagacggt ttcaaggtct atcccgttgc agaggccgtg
     5161 aaatcagccc aggttgtcca ggtcctcgtc ccggacgagc accagggggc catataccag
     5221 gccgagatcc gcccgcacct ctcagagaac aaatgcctga tgttctccca cggcttcaac
     5281 attcactttg gccagatcgt gccaccgcct gacatcgatg tcatcatggt tgcgccgaag
     5341 ggtcccggac acatggtgcg caggacatac gaggagggga agggtgtccc cgcgctcatc
     5401 gccatccacc agaactatac cggcaacgca caacagatcg cgcttgcata cgccaagggg
     5461 attggggcaa ccagggcagt ggtgctcgag acgacgttcc gcgaggagac cgagacagac
     5521 ctcttcggcg agcaggtggt gctgtgcggg ggtatcacct ccctcatcaa ggcgggtttc
     5581 gagacactgg tcgatgccgg gtatgcaccg gagatggcat atctcgaggt cctccatgag
     5641 accaagctga ttgtggacct tatctacgag ggaggcttta cgaagatgcg caactccatc
     5701 agcaacaccg cccagtacgg tgacctcacg cgcggtcccc gggtgatcgg ccccgagtca
     5761 tacctggcaa tgcaggagat tttaaaggag atccagagcg gcgagtttgc cagggagtgg
     5821 atgcttgaga acctggtgaa ccgcccggtc ttcaatgccc tcaccagggc tgatgaggag
     5881 cacctggtcg agcaggtggg aaaagaggtc agggcgatga tgccccagtt caaaaaacaa
     5941 taaagaccca tgtttttttt caatgaaaat gccctgcggc atttccatga atgcatatgc
     6001 gtgtgtctcg atgaaagtca tgtatttttt tcataagtta accggaattc cttttcaatc
     6061 atcacatgaa ggtgtttttt ggtttccagg tcaaaaagcg tgagaacagc gtgaattgag
     6121 agagcgatgc ctgtccccgg catcgcgacc gggccccggg ccttaatcct tggcgatatc
     6181 cgttatgcaa agtgacagca gggagctttg cccccgtgat ccctttactt gcaggcgagt
     6241 cttacggagg agaatggttt gcctgtacgg aagcctgtga aaaagaggaa acgtccgttc
     6301 ccgtgagggg gcatttttta cacctggaca ccttatataa gtactatggt tgagccgtac
     6361 gagacactcc tgaaaaaagc ctattcccac gtgaccgagc cgaccgaatc ggtggagcgg
     6421 ttcacggtgc cggaggcgag gtcgtatatc gaaggaaaga caaccgtgct cgaaaatttc
     6481 tcggagatcg ccgataccgt ccgccgcgac aaggatcacc tgatgaaatt cctcctcggc
     6541 gaactcggca ctgccggcaa gattgacggc acccgcgcca tcttcaacgg gaaatttgag
     6601 gcggcgctga tcaactccct ggtgaagggg tacgtggatg attatgtcat ctgttccgag
     6661 tgcgggaaac ctgacacccg cctcgtgaag gacgaccgtg tcctcctcct gcgttgcgat
     6721 gcctgcggag gtcaccgccc ggtgaggaag aggaaagccc ggactgagcc ggtcacaaat
     6781 gagatctcag aaggtgcgat cctcgacctt gaggtccagt ctgtgagccg gagaggcgac
     6841 ggcgtggtga ggatggggcg gtatatcctg tatatccccg gtggaaagcc gggtcagaaa
     6901 gtcaaggtca ggatcacccg cgtctccggt tccatcgtgt tcacggaacg ggtgcttgtc
     6961 tgaacctggc agtctcagga tggaatacca ataaaatagg ataatcaact ttttctcaac
     7021 aattccgcgc tttacgatct ccccttggag agaatctcaa ggagctcgtc acacgacccg
     7081 gatatcctct ggcaggcttc gcggatcgcg tccagcgggg ctttctttcc attggtggtg
     7141 acgagaagct cagggtccga gaactggaac ttgatgaggt atttcgccac gtccacgtca
     7201 gggtccttca gtatctcctc tgtgaggaca ttcatgaagg tgtggccctc cccctgaatg
     7261 aggagttttg ccttgtcttt ctcgagttcc aggatcttta tattcatcca cctatgaatg
     7321 ggcgggggag tcctatatag gtatgcgtgg gttcctcgca ggcatctgcc gggcgaatcg
     7381 caacacgcga tcagaaagta aaggtctcgc cgtcacatcc caggtgtggg aggccccacg
     7441 gaaggtgatg gctctggtgc acgcaacgga aatcccctgc gccgagcgtg cgtgcaaggt
     7501 cacatgcctc cttgtaattc atgtgcttgg ggacatggag gtgggaaggg agaagtgcgt
     7561 ccacgaagag gaggtctgtt ccttcaagat gcttcgaggt ctcttcgggg agccgcaagg
     7621 ttgtgtcgga ggtataccca atccgcacgc cgtcggcctc cagcagaacc ccacaggtgt
     7681 acgtgggagg atggtccacg gtgagaaacg tgcactccac cccgaagagg gtgaacgggc
     7741 aatatgccct gacagggtgg cgatcgcgaa ggagaaactg gaagatcctc ccgcagtagt
     7801 ccattacagg aaggggcgcg tacacgtctg gcacatcctg gaccctgtag aactcggcaa
     7861 accccatgaa atggtcgtag tggccgtggg tccagagcac ggcatcgata tgcggagatc
     7921 ctgcggcaag gagctgctgg cggaggtcgg gcccgctgtc gatcaggatg tggcggccct
     7981 gacgctccac cagcaacgat gtccgcagcc tctgcaggcc atgttcgtgc gcatgggtgc
     8041 actgggtgca cgaacacccg atcctgggag taccgatcgc atcacccgtg cccaggagga
     8101 cgatttgcat cctatgacaa ccagttctgg gaacggatgg tgtccctgtt cttcaccacc
     8161 gtgatgccac gctcgatatc ggtaatattg attgcatccc ggtgatccat cagcgaagag
     8221 aggagcgcct ccttgatcat gatacggaga tcggcccccg agaacccttc cgttctttct
     8281 gcaaggtccg ggatatcgaa catgtactgc atgccagtgg agaccttctc gaggattgcc
     8341 ctgcgcatct ccctgtccgg gagcccgaac tcgaccacct cgtcaaacct cctccaggca
     8401 gcctcatcga gcagctgcgg gtgattcgtt gccccgacaa ggaggacgcc atgccggatc
     8461 aggctcacct ggtcaatgtt cttcaaaagc gagttgaccg cccgcttcat tgcaccatgg
     8521 tcatcggcaa ccctgctctt tgccacgaag tcgaactcgt cgatgaacag gatacaggga
     8581 gagagggctt ttgccagttc gaagatccgg tcgatgttct tcgaggtctc accgaggtac
     8641 tgggaggtga tcatcgagag gcgaacctcg aggaggggaa ggtggagttt ccgggcgagt
     8701 gccagggcaa gggaggtctt gccggtgccc ggggggccca cgaagaggag cttgcccacc
     8761 tcgtggatgc ggtgcctgcg gaggatatcc ctgtgcgttc ttgcaacctg gatcttcctg
     8821 atgatgttct cctgcgctct cgtgcagacg aggtcgttga agtcctgctc gacctcctcg
     8881 ggcgcgctca atattatcag gtccatcgcg ttcttcaggc gctcgtcgtc cgcgagtatc
     8941 ttcgtgagcc tggactgcag gaacacccgt gtgtcttcta tgggggggtt atcctggcgc
     9001 gccttctcgt aggatacctt cgtcgattca aaatgctcaa agaaaaaggc cagggtagga
     9061 ttcttctcta tctgcggcat gccgcctatc ttcaggaacc agcgcgccgc aggctcgagc
     9121 gcagtcagct ttatacgctg cccgaactcc tcgcaggtga ccaccggcgc ctgctcgagc
     9181 tttctgcagg cgttctcgat accgaggctt ctcctgacca tcccctcgct cacgtgcagg
     9241 ggccgcttca cctccgccgt gccattgact gtaaagaggt tcctgcattc cggggtcagg
     9301 tcgtttgcgt caaggttctg gtgggagttg aagagttctg cggtgaggag gatctctatg
     9361 agcttgaggg tctcgtcatc agcagccatt tctgtaactt ttactatttt tcctgggata
     9421 gtttaaggat atctcatgcg gtggtgacga ggcacccctc gcttgtgacg atgagtgtgt
     9481 gctcggcctg ggaaacgagc gaccccggga catcggagag gacagggtat gcacgtatca
     9541 cctgtgagcg gacaagagtg gagagggcaa ggtcgagttt tccctcattt aaccatctcc
     9601 ttgagaaagg catgccctgg cgttgcctga tctcctcgag cacacggcgg gcggacggca
     9661 gtctcaccgg ctttactgcc agttgctggt agatctcggt gcggtgcttc tcgctcacct
     9721 ggccgatccc ggtactggca aaaggctcga tggcaaagac catcccctcc tcgaggacag
     9781 ccccgccgtt gagacggata ttggggatgt tcgggggagt atgtattctg tacctgtcca
     9841 gtccgtggcc ggtgaggttt gcgaccggtc gaaatcccct tgactcgatc tcctcgtgga
     9901 ttgccgaccc gagttctccc acggtgacgc ccggcgcgac catcgagatc gccgaggcca
     9961 gtccgcgcgc tgaggcctcg acgaggagcg ggtggtcccc gaggtcgact gtcatcgcgg
    10021 tatcggcgat gtagccgtct acgtgaacac cgaggtcgag cttgacaacg tctccctgtg
    10081 caaacaccct ctcgtcgccg gctgaggcgg tatcgtgcgc ggcgtcctcg ttgagagaga
    10141 cattcagggg gaatgcaagg ccggcaccgg cgtcccgcac catctcctcg atcgtatcca
    10201 ctacggcaag gtatgacgcc cccaccctga tctcgcctgc acctttcctg cgtacttccg
    10261 aggctatctt tcctgcctcg atgtacttgt ccatcacttc gtctttcatt aggtcccccc
    10321 tcacactgcc aggtctcttg ggtaccgggt tagggtcgca aacccgcctg ggccgatgac
    10381 tccaatgttc tcaagccgta cgccgccgat cccgggcatg tagaggccgg gctcgttggt
    10441 gaccacgttc cccggcagca gaacctctcc ggagggcccg agactcggtg cttcgtggac
    10501 ctcgaggccc acgccatgcc cgaggttgtg cacgaatccc gagctgccgg tcccgtatcc
    10561 agtctcttcg aagaagccga ccaccgcgcg gtagacctct tcaccggtga cgccggggcg
    10621 aatcgtttct gcggcgaggt cctgggcgtc cctgaccgcc tggaacatct caaggacatc
    10681 gggggatggc tctccactgc agactgtacg gctcatatcg ctgtagtagc cggtctgctc
    10741 gtctcttggg aagatgtcga tcacgatcgg ttcgtctgct ttcagcgccc cctctcccct
    10801 gagatgggga agtgcgctct cctcgccgca ggagacgatg gtatcggtag ggatgcaccc
    10861 tctcttcagg agaacctggt gcatagccgc ccggaccatc cctgacgtga gggggaccgc
    10921 tcccatgtag agaagccccc ccttcaccct ggccttcctg atcacctgga tcccgcggtc
    10981 cattgcctcc tcggtggcag cctgcaccgc actgatcctc cctatctccc acgggctttt
    11041 tgctgccctc atcctggcga gtgcctccct gtccacctcg acagccacaa gttccgagag
    11101 ttctcttcca agggcatacg ggaattgggg ggggatcagc accttgccct gcacgaggcc
    11161 agcgatcatc cgggcaagcg cccgccatcg gtcttgctcc cctttcagta tctcgaacag
    11221 cccggcctgg gcccgggaaa tcacagcagc agttgcctct ctctcggccc gttcgtattc
    11281 catctgtgac accacgacag tccccttctc cccgggtttg cggatataga caattggatc
    11341 tgtagtggaa aaccgggtga gatagcgcat gtcggcgtcc tgagatgagc cataggccac
    11401 gtaggccgtc gacccgctcc gcaggatcgc ctcttcgagc gcatccatat gccatcctta
    11461 ttggaggcga tcccacaagt acttttatca tcgcgcagat attcctgtaa tggacgaggc
    11521 cacaaaagag cagctaaaat ggaaatttta ccgcctggcg atcatcctca atgcgatcgt
    11581 gctgctggtc gccctgggag tcatcgcaat cctgaaactc ccggaaccgg tggcgcttcc
    11641 cggagggatt gccctcattc tcctggcagt ggggctcgca atctatttta ggaaacagta
    11701 cgtctccacc aaaaagtggc tcgatgagca ggcctcgaag gaccgggccg gggggcatgg
    11761 tcaatgattg agaaggtgaa aggagagatc gaactgatgc agcggcatat ccaggtgctg
    11821 cggacagtgg tggcccacca gcccatcggc atcatgaaac tctcggagtt aatggacctc
    11881 ccttttcaca ggatccgcta ctccctccgc gtgcttgagc agatggggta catccgggca
    11941 tctcaatccg gtgcggttgc aacaccccag agcgtggagc tcctcgcggg ccttgaccag
    12001 gagcttggcg agatgatccg tctcctcgag agcatgagac accagggcgg ctgatctccg
    12061 aaaagtccgg cggatcccta ttgtccatcc ctgcatgaat ctccgctaca tttaacaccg
    12121 aaagcggcaa cctatattgg cacatcggat tgccgccata actcagttgg tagagtggct
    12181 ggctgttaac cagcatgtca caggttcgag tcctgttggc ggcgtttttt cttccatggg
    12241 cccgtagctt agtccggtca gagcgcccgg ctcataaccg ggcggtcatg cgttcaaatc
    12301 gcatcgggcc cactttaaat ccctcccgtc ctggcacccg ccattgccgc cgtgttcaaa
    12361 aaggttagta tggggtccgg tccaatttcc caggtatgaa gtgcccggta tgcggggatg
    12421 gatgcatcca ggatgcaccg gcggtcctcg aatcccttgc ctctgtcttc tcccggtgcc
    12481 ccaggtgcac gttcagggtg ctcgataaaa gcgccccact gaagggacgg tattatggga
    12541 caccctgcga atgcgggaag cggtttatcg acgaggtatt tgcccatctc tacgtgatcc
    12601 tggtggagga gggaattttt tccgggagcg agccgctcag ggcggtggga tcccctcttc
    12661 tccaccctgg cttctttatt gcgggccccc cgttcctccc cccgggatcc ctcgtcctcc
    12721 tctcgcccct tgtgacggaa tccgctgcac ggcgcatgat tacagaggtg ccggaggttc
    12781 gcggcgtggt gaggtctggc gactttgttc ctggggccac cgatccttca cttgccactg
    12841 ttccgcgtag ttacgaactt cttgcaggtt gcgatgtgcg ggcgggtatc tttcccacgg
    12901 gggcaggccc cctggtcata tatacccagc agtcccagat gcacatcgag tttccgcgcc
    12961 tggagaaccc caagatccag tccgtggaac gccacattgc cgcacagcgc cccacgtggt
    13021 gcgtggatgc ctgctcgggg gcggggacac tcgggctctg cgctgcaagg catggaatac
    13081 ccggggtgat tctcaacgat gcctggtatt cagccgcgtt ctggtccgcg acaaacctcc
    13141 acgtcaaccg gatgttcttc caggtcgaag gggtacggat acacctgtcc ttccgggaga
    13201 tgagcatgca tcccgtgggg agggatccgg tcaaggtcgc ggaggcatcg ggagggcagt
    13261 caatcgctgt gtaccacggt gatttccggc aactctggaa ggtcctgcca cgtgaacctg
    13321 tctgtgcggt tctcgatctc ttcgagaaat cggacacccg gatgaccgaa gcagcaatga
    13381 aggaatggca gagccgcgtt cccggcgagg tatttatccc ctagggtcgc gatatgttat
    13441 ggggactagc ccgggtggcc cggcgtcacc tgtgacccga aaccgtcggc atgcgggggg
    13501 cgaagttcgg agaaggcggt aacgggctgt ctgtgaatgg tgcaggccaa cgttgaaacc
    13561 tcgtcccatg aggtcggtgg acagggatca aagccccgga gggggcggcg acctgttgtc
    13621 gcggggaccg gttcaggccc ggaagggagc agacttaccg tgaacattcg gcgcccatgg
    13681 ggt
//
LOCUS       DAOQYR010000061        13550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_5050_length_13550_cov_7.765617, whole genome shotgun sequence.
ACCESSION   DAOQYR010000061 DAOQYR010000000
VERSION     DAOQYR010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 13550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13550
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5050_length_13550_cov_7.765617"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1621
                     /locus_tag="PKM87_08035"
     CDS             173..1621
                     /locus_tag="PKM87_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNQ30052.1"
                     /translation="MSETVTRPKMSPKDLMIVLVISLGSFMAGLDATIVNIALPTISK
                     AFDVSTVTSSWVLNAYLIVLVSLLLASSRLGDIRGYRGVFLAGFLVFTVGSGLCGLAS
                     GISVLIASRMLQAVGGAIIAALGAVMVTSYLSPSLRGQALGIVAMFTMLGAALGPVLG
                     GFLTSAFSWRFIFYVNLPVGILAILLGMHYLPRKNPVAPSAKLDIPGVMLVFLALGTL
                     IYGLTTLQGERGLLGALSLSGSLVFWILFVIREKRAAEPLLNLALFRNRQFTLQNFNV
                     MCIQLAMAGVMILMPFYLEMVKHLPTDNAGTILLALPIGMILTSPLAGRISDVIGTKK
                     PIILGFVIGTLALFLLATLGPQSSIGHAEIYLFLLGAGSGFAYAPLNSAVMGESPPKD
                     RGSTSGLIKMMTNLGSSLGVALVMLVATAAMGPALAHSSAHTLPPVELAGAFHVAFLF
                     LMCIEILGILLMLPVKEKESGYSTEGEPVTAF"
     gene            complement(1632..2147)
                     /locus_tag="PKM87_08040"
     CDS             complement(1632..2147)
                     /locus_tag="PKM87_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30053.1"
                     /translation="MFKTIAHIYDSDDPSPEASRELTERAEEVICSAVLDTRKPLQAA
                     GDYLLDIRIPAPDYTFQRENDISSAIRAFFLKRADEIHRERRLSGRVGLREFQLTIAV
                     CVPALLGIVACDPFPHEPLAIILQNVLLIFCWVVIWQPFQSLVFDRWTLAEKEKVYRQ
                     IAGMEMRVLPA"
     gene            2368..3348
                     /locus_tag="PKM87_08045"
     CDS             2368..3348
                     /locus_tag="PKM87_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNQ30054.1"
                     /translation="MTGGGIGSSVSGATEGGDGHAYPEIEKRYTHVRARIHRLFDEPP
                     GDDRLAFGIQAFLAAVVLLNTVTVVIYTVPWLEEEYWLPLNGIMTACLLVFTIEYVVR
                     LWACTSAPTLAARTTYRIQYASSFYMIIDLVSIVPLVFPLFFPRDFALLRAFRLLSIF
                     KLGRYARKSQSLALLKRVVMRKREIFTLMVFFLVFVILFSSTIMYLVEHQAQPEKFSS
                     IPAAIWWSMMTVTTVGYGDIYPVTPLGQSIASLFTLGGVLLLALPSAILASGFIEERH
                     KQGESGLGIPLEREIEMLEKVGHLHEKGVISEKELEEYRHTIRILAGKKG"
     gene            3621..5621
                     /locus_tag="PKM87_08050"
     CDS             3621..5621
                     /locus_tag="PKM87_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HNQ30055.1"
                     /translation="MIEVDVFLFDILIVLGLSILVLFLGNKFRVPGIVGFILAGMLAG
                     PYGLGLIQDQEIIQFLAEIGVIFLLFSIGMQFSFRTLYHMKKIVLLGGALQVGFTILV
                     TAGLAYMLGFPLPSAIFLGFLICHTSTTITLRVFQDRGELDSPHSRTTLGISLFQDLM
                     SVPMILLLPALSGHDIQPGEFAITVEVALVASLVLMLVLLFYLLPRLMDTITRTRSQE
                     LFLLTILFVCLAITWITSSIGLSLALGAFLAGLLLAETPYFHQAFATILPFRDIFTSF
                     FFISIGMLLNAHILITHPLIVLGLIAGAIILKAVIAGLSSLSLGQSLRTSVLAGIALA
                     NIGEFSFVLILAGMEFAFLSDFSTQVFLTVAVWTMALSPFLISAGPRISGWICDLPLP
                     QRIKAGSEPPPGTLPATHRDHLVIVGFGPNGRNLARVAEMEGIPYIVIDMNPDTVAEE
                     RKKGLPIFFGDAANPSILDHAGIEHARVLAIVTNDPFSSRTITAMAREMNPGIHILIR
                     TRFLRDIDQLREIGADEVIVDEFETSIELISRVMKKYLAPRVTIDRATSRIREDMYRV
                     FRQPPSDRGIFSEIALRIPQVEVQTFQVAERSPVAGHTLRELKVRTEFGVSVIAIGRN
                     EDTIPNPDGEFRVDAGDRLVVIGKPDQLASFAAIVEMDGTGE"
     gene            complement(5863..7056)
                     /locus_tag="PKM87_08055"
     CDS             complement(5863..7056)
                     /locus_tag="PKM87_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNQ30056.1"
                     /translation="MEGIIATPEFQVSLLLFAALGGYLLASRINQSAVVGIILVGLLI
                     GPSVLGLVSYNEFVRGLAHLGAVVLLFVIGFEFKLRDILKPSYALIALVGVIIPWVGG
                     YATAIWAGMDFASGLFIGTALTATSIAITANVLQELDKLQTGPARAIIGAAVIDDVLS
                     LVALAITGDVVAGAFSPAVIFFILLKVAIFLVVAGAVGIFIVSRFLVRLDQTSFVAKY
                     PEFIFIFAMMFAFLYAMIAELVGLSAIVGAFIAGISFERLSLRKSLDVKEGAEFLKII
                     FAAIFFVSLGVVADIKAITPEIVPFLVVLTVVAIVTKVVGCALPARAAGMCTKDSLII
                     GFGMAPRGEVAMIVALIGLEQEYIGQEVFVIIILMSLITTLITPIVYRNWFYKEEYCK
                     PEIEI"
     gene            complement(7171..9147)
                     /locus_tag="PKM87_08060"
     CDS             complement(7171..9147)
                     /locus_tag="PKM87_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNQ30057.1"
                     /translation="MELAILYDILVIFGLSLLVGLLFSRIRIPPMIGYIVAGAIAGPS
                     VLSLIHSPAQVDVLAEIGIILLLFSIGLEFSFRQLWEIKGLVLKGGAIQVALASAATI
                     AVVFLAGRPWNEAVLLGFVVSLSSTAVVLKVLHDRGELDSPHGKLTLGILIFQDLVAI
                     PMMMAVPFLADTGNGLSEPIYLMLLKDLLLVIILVAAAKWVMPWLLFQIARTRSSELF
                     LLFVVSVCFGVAWLASFAGLSLAMGALLAGLLISESEFSHQAIGRIVPFRDIFTAFFF
                     VSIGMLLDVGFFFDHVVLVLVFLAIVLLLKFLTAAAAPIVLGYQMRTIILAGIALAQV
                     GEFSFIITKSALDFGILSHEVYQVFLIVALGTMALTPFLIASGPSLAGYVCSHSLFMR
                     LFPARCQWIAREERVARSGHLVIIGYGVNGKNLARAAKMGGIEYAIVDMNPDSVRHAT
                     LEGEPIIYGDATTEGVLDHAGIDTARIAVVAINDPVATRRIVGLCRQKNPGIFLITRT
                     RYLVEVPVLREIGADEVIPEEFETSIEIFMRVLRAYGVTGDSVEHLVRDIRADNYQAL
                     RSTSAPPVVAALDIPGMEVREVTVEDGSPIAGKTLGELLLKKRYGVTVLAIRRNGALI
                     PHPDGTCAMRAGDVAVLTGTIEKIEEALVLFLSP"
     gene            9540..10016
                     /locus_tag="PKM87_08065"
     CDS             9540..10016
                     /locus_tag="PKM87_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30058.1"
                     /translation="MVRVKDIPMPTRWAIATQALTHLVMAADPQQCRDNPAYLMDELG
                     EEIRGIAGRYQMPREDAGGLVQTLGAISVILFGPTFETPYIEGFHEESVIRLSECAMF
                     RPEREKKRDPSHVNQVCTAYVMSAIRALNPEYTIRISRARCRGDSFCEMIIEKKKP"
     gene            10106..12868
                     /locus_tag="PKM87_08070"
     CDS             10106..12868
                     /locus_tag="PKM87_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNQ30059.1"
                     /translation="MKKEREHINHPVPPWHALPIEQVYETLISDGRGLSREEAARRLV
                     QYGKNSLPAREAPGIAEIVLHQFKSPLIYILLAAGIISILIGDVKDAGFIFLVVIINA
                     AIGTAQEWKAEQSAMKLQSLLQVMSRVMRGGTWQMLPAEDLVIGDVVQLESGMRVPAD
                     LRLLHAANLAIDESLLTGESVAVEKAVGVRDENIPVSDRSNMAYAGSTVVTGRGCGIV
                     TGTATRTEVGMIAEAVSGTESAKPPLIIRMEAFSQKVGILIIAASIVMAIVALSRGTP
                     PIEVFFLAVALVVSAIPEGLPVAITVALSIAASRMAQRHVIVRKLAAVESLGSCTTIA
                     TDKTGTLTVNQQTARVLFIAPGAYAEADGVGYDPGDGRITAEGVPLQGPLSDIVLRLA
                     RAAVICNEGALYREDGTWVHHGDAMDVAFLTLGYKTGLEPDAVRNAIRTVATVPFESE
                     RMYAAVYYRDDDGTQGGKVKIAVKGALEAVLPYCTSMATPAGVVPLDSAGVEEHLIRM
                     MEDGYRVLAVAEGELSEPPGQNAELSDVRPRLTLLGLAGFIDPLRPDVKDSVRLAHHA
                     GIDVVMITGDHPRTAFAIARELGIAQSFSEVMTGQQLGAMGDPNLPAFAEAIDRVRVF
                     ARVTPVQKMEIVDTLVRRGHFVAVTGDGVNDAPALRRANIGVAMGSGTDVAKDTSSMI
                     VTDDNFSSIVAGVEEGRFAYDNIRKVTYLLISTGLSEVVLFILALIAGLPLPLLAVQL
                     LWLNLVTNGIQGVALAFEAGEKGTMRRKPRDPKEGIFNGLMVKETALSGMTIGVVAFL
                     FFGWMIGSGVEESIARNLLLLLMVLFENFHVFNCRSEWRSLFRVPLRNNAFLVIGVIL
                     MQGLHILSMNLPFMQDLLSLSPVTLDQWLLCLVIAASVVVVMEIFKYVSHRSMPDAAS
                     RGGVAV"
     gene            12966..>13550
                     /locus_tag="PKM87_08075"
     CDS             12966..>13550
                     /locus_tag="PKM87_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNQ30060.1"
                     /translation="MTPEREHWSSGAGFVLAAIGSAVGIGNIWRFSAVVGQNGGGAYL
                     VPYLIAVFAFAMPLMILELAMGRHFQGTVVSAFCSVRAEFRVLGWLLCAIIFLVLSYY
                     LVIAGWTAAYALFSLSGETVPFASFTGGVLPVLFFLFTAVITAAVISKGIQKGIERLS
                     VVLIPISLSILVILALYNATLPGFAEGILFLFTPD"
BASE COUNT         2805 a         3901 c         3942 g         2902 t
ORIGIN      
        1 tttcgggtcc tggtagtgaa ggtgccgcgg ccgacggaag agacaaaact cctgtgatct
       61 ttccggcagc catacccgct ttttccccga tctggtaatc cctgggccgg gatccatcat
      121 acactcgatt cacagactat attatcaatt gttgtgatga gttgatgaga acatgagtga
      181 aaccgtgacc cgtccgaaga tgtccccgaa agacctgatg attgtccttg taatctccct
      241 tgggtctttc atggcagggc ttgatgccac catcgtgaac attgcgctgc ccacgatctc
      301 gaaggcgttt gatgtctcaa cggtgacctc gtcatgggtt ctcaatgcgt atctaatcgt
      361 ccttgtgagc cttcttctcg catcatcacg cctcggtgat atcaggggtt accggggtgt
      421 cttccttgca ggtttcctgg tgttcaccgt tgggtcagga ctctgcggac ttgcgtctgg
      481 tatcagtgtg ctcatcgcct cccggatgct ccaggcagtg ggaggcgcaa tcatcgcggc
      541 actcggggcg gtcatggtca cgtcatacct ctcgccctcg ctgcggggcc aggcgctcgg
      601 gatcgtggcc atgttcacga tgctcggggc ggcactcgga cccgtgctgg gcggctttct
      661 taccagtgcc ttctcgtgga ggtttatttt ctacgtgaac ctgccggtcg gtattctggc
      721 aatcctcctg gggatgcact acctccccag gaagaacccg gtcgcaccgt ccgcaaaact
      781 cgatatacct ggtgtgatgc tggtcttcct tgcactcggc accctcattt acggccttac
      841 gactcttcag ggtgaacgag gactcctggg ggccctgtca ctttcgggat cgctggtatt
      901 ctggatcctc ttcgttatac gcgagaagag ggccgccgag cccctcctca acctggcgct
      961 cttccggaac aggcagttca ccctgcagaa cttcaatgtc atgtgtattc agctggcaat
     1021 ggccggtgtc atgatcctga tgccattcta tctcgagatg gtcaagcacc tgcccacgga
     1081 taacgccggg acgatcctgc ttgcccttcc catcgggatg atcctcacct cccccctcgc
     1141 cggcaggatc tcagatgtca tcggcacgaa gaagcccata attctgggat tcgtgatcgg
     1201 gacactcgca ctctttctcc tggcaacact gggaccacaa tccagcatcg gccatgccga
     1261 gatctacctc tttcttctcg gtgcagggtc aggatttgca tacgctccgc tcaacagtgc
     1321 ggtgatgggg gagtcccctc caaaggaccg tggcagcaca tcaggcctga taaagatgat
     1381 gaccaacctc ggatcctcgc ttggtgtggc cctcgtgatg ctggtcgcta ccgctgctat
     1441 gggcccggct cttgcgcatt cctctgcgca cacccttccg cctgtagaac ttgcaggtgc
     1501 attccacgtc gcgttcctct ttctcatgtg catcgagatc ctggggatac tcctgatgct
     1561 ccctgtgaag gagaaagaaa gcgggtactc cacagaggga gagccggtca ctgcctttta
     1621 aaaggaggag atcaggcggg aaggaccctc atctccatcc ctgctatctg cctgtatacc
     1681 ttttctttct cggcgagcgt ccagcggtcg aagaccaggg actggaatgg ctgccagatc
     1741 accacccagc agaatatcag caggacattc tggaggatga tcgcgagggg ttcatgggga
     1801 aagggatcac atgccacaat cccaagcagc gccgggacac acaccgcaat ggtgagctgg
     1861 aactccctca gccccactct cccggaaagc ctcctctccc ggtgaatctc gtcggcgcgc
     1921 ttaagaaaaa atgcccgtat tgctgaagag atgtcattct ccctctgaaa cgtgtaatcg
     1981 ggtgcgggga tgcggatatc caggagataa tcccccgctg cctgcagggg ttttcgggtg
     2041 tcaaggactg cgctgcatat gacctcctca gcccgttctg taagttccct ggaggcctcg
     2101 ggtgaaggat cgtcagagtc gtaaatgtgg gcaatagtct tgaacataac atgtagaggt
     2161 tctttatcat tagagtgcga caggatctgc atggaaaatc tcccgcaatt ctcttttctg
     2221 tagtgtgttg aagatgatga ggatttttgg ccagaaccag gtgcagcacc ggaatgcacg
     2281 cgggagatgg atagccagtg aaagcgtccc ttagaagatg caataagagg tgaaacgtta
     2341 agtaagagag ccgcacacct cacgcatgtg acgggcggag gaattggatc atccgtatcc
     2401 ggggcgacag aaggggggga tggtcatgca tacccagaga tcgaaaagag gtatacgcat
     2461 gttcgtgccc gcatccacag gctctttgat gagccgcctg gagatgacag gctggccttt
     2521 ggcatccagg catttcttgc agcagtcgtc ctcctcaata cggttacagt ggtaatttac
     2581 accgtcccct ggctggagga ggagtactgg ctccccctca atgggattat gaccgcatgc
     2641 ctgctggtct tcactatcga atatgtcgtg cgcctatggg catgcacgtc cgcacccacc
     2701 cttgcggcga ggacgacata tcgtatccag tacgcatcca gtttttacat gatcatcgat
     2761 ctcgtatcca tcgtgccgct ggtcttcccc ctcttcttcc caagggactt tgccctcttg
     2821 agggcgttcc gcctcctgtc aatctttaaa ctcgggcgct atgcacggaa atcccaatct
     2881 cttgcccttc tcaagcgggt tgtcatgagg aaacgcgaga tcttcacatt gatggttttc
     2941 ttcctggtct ttgtcatcct tttctcttcg accataatgt atctcgtgga acaccaggcc
     3001 cagcctgaaa aattctcgag catccctgct gccatctggt ggtcgatgat gaccgtgacc
     3061 actgtagggt acggtgatat ctacccggtc accccccttg gacagagcat cgcgtctctc
     3121 ttcaccctgg ggggagtgct cctcctcgca ctcccttccg cgattctcgc gagcggtttc
     3181 atcgaggaac gccataaaca gggtgaatcg ggtttgggga tacctcttga gcgggaaatc
     3241 gagatgctgg aaaaagtcgg ccatctgcat gagaaagggg tgatctcaga gaaggaactg
     3301 gaggaataca ggcacactat ccgcattctc gctggaaaga agggatgata accccttcag
     3361 aagaggatct ctctgtcgtt cgacctgttt gggatccccc ggcgattacc cgtcctggct
     3421 aaacaggcag gtttactttt cctacaccag caagagatcc attgctgttt tttttatgag
     3481 gggatttcct ggccatcttc aggcgagtgt attcaaggga ggagatggca gcgccggccc
     3541 gtgatgcagc agcggttctg acacaggcag gctgatgaac tccatcgtct aattaggtag
     3601 ggagagctgg tggtgattcc atgatagagg tcgatgtctt cctgttcgac attcttatcg
     3661 tccttggcct ctccatcctg gttctcttcc tcggtaacaa gttccgggtg cctggaatcg
     3721 tcgggttcat cctcgcgggg atgctcgctg gcccgtatgg tctcggcctt atccaggacc
     3781 aggagattat ccagttcctg gccgagattg gcgtaatctt tcttctcttc tccatcggga
     3841 tgcagttctc gttccggacc ctctaccaca tgaagaagat cgtcctgctg gggggagccc
     3901 tccaggtggg gttcaccatt cttgtcactg cgggactggc atatatgctg ggatttcccc
     3961 tgccctccgc gatattcctc ggtttcctca tatgccacac cagcaccacc atcactctac
     4021 gggtcttcca ggaccggggc gagctcgaca gcccgcacag ccggacgacg cttggcatct
     4081 ctctcttcca ggacctgatg agcgtcccga tgatcctgct ccttcccgct ctctcggggc
     4141 atgatatcca gcctggggaa tttgcaatca ccgttgaggt ggcgcttgtc gcatcacttg
     4201 tcctgatgct cgtgctcctc ttctaccttc ttccccgcct gatggatacc atcaccagga
     4261 ccaggagcca ggaactcttt ctccttacca tcctcttcgt ctgccttgca atcacctgga
     4321 tcacatcgag catcgggttg tcacttgcac tcggtgcgtt ccttgcgggg cttctccttg
     4381 ccgagactcc atattttcac caggcgttcg ccaccatcct tcccttccgt gatatcttca
     4441 ccagtttctt cttcatctca atcgggatgc tcctgaatgc gcacatactg atcacccacc
     4501 cgctcattgt acttggattg atcgcagggg caattatcct caaggcagtg atcgccgggc
     4561 tctcgtcact ctcgctgggc cagtctctcc gcacctcggt gctcgcaggg attgcactcg
     4621 caaatattgg ggagttctcg ttcgtcctca tccttgcagg aatggagttt gcgttcctct
     4681 cggatttctc cacccaggtc ttcctgaccg tggctgtctg gactatggcc ctctcaccgt
     4741 tcctcatctc cgccggcccc cgtatctccg gatggatatg tgatctcccc cttccccaga
     4801 gaatcaaggc aggttccgaa cctcctccgg gcacgttgcc cgccacccat cgcgatcacc
     4861 tggtgatcgt tggtttcggg ccgaacggga ggaaccttgc ccgggtggca gagatggagg
     4921 ggatacccta catcgtcatc gacatgaacc ccgatacggt ggcggaagaa cgaaagaaag
     4981 gcctacccat cttcttcggc gatgcggcaa acccctcaat tctggaccat gccggaatag
     5041 aacatgcgag ggtccttgcg atcgtcacga atgacccctt ctccagccgg accatcaccg
     5101 cgatggcccg ggagatgaat cccggaattc acatcctgat caggaccagg ttccttcgcg
     5161 atatcgacca gcttcgggag atcggcgccg atgaggtgat cgtggacgag ttcgagacct
     5221 cgatcgagct catctcaagg gtgatgaaaa agtacctcgc tccccgggtg acgatagacc
     5281 gggcaacctc ccggatacgg gaagacatgt acagggtctt caggcagccg ccttctgaca
     5341 ggggaatatt ctcggagatc gcgcttcgca tcccgcaggt agaggtgcag actttccagg
     5401 ttgcggaaag gtcgcccgtg gcgggccata ctcttcgcga actgaaagtc cggacggaat
     5461 tcggggtctc ggtgattgca atcgggagga acgaggatac cattcccaac cctgacgggg
     5521 agttcagggt ggatgccgga gatcgccttg tggtaatcgg aaagccggat caactggcgt
     5581 catttgccgc gattgttgag atggatggca caggcgagtg accaccctgc ctcggcggtt
     5641 cctgcctgaa aaaatggctc ttcgctatct ggtgtggata gggttgttga acaggacaca
     5701 ttttgtgggg gctgtccgcc cccacactcc catatgcaat gcacgccgcc gcccccgacg
     5761 gggcgccccc gcggtgagtc agtgactgaa ttgatctgca gataacaacc tcgattcagt
     5821 ctccccacac aaaacagcga ggaggcaaaa aaattggagc gtttatatct ctatctccgg
     5881 cttacagtat tcctccttgt agaaccagtt ccggtagaca atgggagtga tcagcgtggt
     5941 gataaggctc atcaggatga tgatcacgaa cacctcctgc ccgatgtatt cctgttcgag
     6001 cccgataagt gccacgatca tggccacctc cccgcgggga gccatgccaa aaccgatgat
     6061 gagggagtcc ttggtgcaca tccccgcagc ccgggcgggc agggcgcagc ccacgacctt
     6121 tgtgacgatg gcaactactg tcaggaccac gaggaacggg actatctcgg gggtgatggc
     6181 cttgatgtcc gcgaccaccc cgagcgagac gaagaatatg gcggcaaaga tgatcttcag
     6241 gaactccgcc ccctctttta cgtccaggct cttgcggagt gagagccgct cgaacgagat
     6301 acccgcgata aatgccccca cgattgccga gagccccaca agctctgcga tcatcgcata
     6361 gaggaaggca aacatcatcg cgaagatgaa gatgaactcc gggtactttg ccacgaatga
     6421 cgtctggtca agccttacca ggaacctgct gacaatgaag ataccgacgg ctcccgcgac
     6481 cacgaggaat atcgcgactt tcaggagaat gaagaagatg accgccgggg agaaggcccc
     6541 cgccaccacg tcgccggtga tggcgagcgc gaccaggctg agcacatcgt cgatcaccgc
     6601 ggccccgatg attgccctgg ccgggccggt ctggagcttg tcgagctcct ggagaacatt
     6661 tgcagtaatg gcgatgcttg tcgcggtgag cgcggttccg atgaaaagcc cgctcgcgaa
     6721 gtccatcccg gcccagatgg ctgtggcata gcccccgacc caggggatta tcacccctac
     6781 gagtgctatc agggcatacg aaggcttgag gatgtcccgg agcttgaact cgaaaccgat
     6841 gacaaaaagc aggaccaccg caccgaggtg ggccaggccc cggacaaatt cgttatagct
     6901 caccagcccg agcacgctcg gccctatcag gagcccgaca aggatgatgc ccaccaccgc
     6961 ggactggttg atccgggatg ccagcaggta tcccccgagt gcggcgaaga ggaggaggct
     7021 cacctggaac tccggggtgg cgatgatgcc ttccatgccg atccattggg agttacgttc
     7081 agaaaactct tctgtgatca cgggggcggc tggaatatag tccccggatg tgagcgtggg
     7141 ggtgccggca gggtcccgcg tggcttttac tcagggactc aaaaagagga cgagggcctc
     7201 ctctatcttc tcaatggtcc cggtcaggac tgcgacatcg cccgccctca tggcacaggt
     7261 gccatcgggg tgcgggatga gggccccgtt tcgccttatc gcgagcaccg tgacaccgta
     7321 ccgtttcttc aggagcaact ctccgagggt ctttcctgcg attggggacc cgtcctcaac
     7381 cgttacctcc ctcacctcca tgccagggat atcaagtgct gccacaacag gcggggcgga
     7441 ggtgctgcga agggcctggt agttgtccgc acggatatcc cggaccaggt gttcgacaga
     7501 gtcccctgta accccgtacg ccctcagcac ccgcatgaag atctcgatcg aagtctcaaa
     7561 ctcttcagga atgacctcgt cggcgcctat ctcccggagg actgggacct cgacaaggta
     7621 gcgggtacgg gtgatcagga atatgccggg gttcttctgt cggcacagcc cgactatgcg
     7681 tcgtgttgcg accggatcgt tgatagccac caccgcaatg cgggccgtgt ctatcccggc
     7741 atggtcgagc accccctcgg tcgtcgcatc gccgtagatg attggttccc cctccagtgt
     7801 tgcatgccgg actgagtcgg ggttcatatc gacgatagca tattcaatcc cgcccatctt
     7861 cgccgcccgt gcgaggttct tgccgttcac gccgtagccg ataattacga ggtgcccgga
     7921 ccgggccacc cgttcctccc gcgcgatcca ctggcagcga gcgggaaaga gcctcatgaa
     7981 aagggagtgc gagcagacat agcctgcaag ggaggggccg ctcgcgatga ggaagggggt
     8041 gagggccatg gtgccgagcg cgacgatcaa aaagacctgg tatacctcgt gggagaggat
     8101 gccgaagtcg agtgcgctct tggtgatgat aaaggagaac tccccgacct gggcaagggc
     8161 tatccctgca aggataattg tcctcatctg gtacccgagg acgatcggtg ccgcggctgc
     8221 ggtcagaaac ttgaggagga ggacaatcgc gaggaagacg agcacgagga cgacgtggtc
     8281 gaagaagaac cctacgtcga gcagcatccc gatcgagaca aagaagaagg cggtgaagat
     8341 gtcccggaac ggcacgatcc gtcctatggc ctggtgactg aactcagact cagagatgag
     8401 gagccctgcg agcagggcgc ccatcgcgag cgagagcccg gcaaaggagg cgagccacgc
     8461 gacgccgaaa cagaccgata ccacgaaaag caggaagagc tcgctgctcc tggtccgtgc
     8521 aatctggaag aggagccagg gcatgaccca cttggcggcg gcgaccagaa tgatcacaag
     8581 gaggaggtcc ttcagcagca tgaggtaaat tggttcagag agcccgtttc ctgtatccgc
     8641 gaggaagggc acagccatca tcatcgggat ggcgacaagg tcctggaaga tcaggatgcc
     8701 gagcgtgagc ttgccgtgcg ggctgtcaag ttcaccacgg tcgtgaagca ccttgagcac
     8761 gactgcggtg ctcgagaggg agacaacaaa cccgaggagg accgcctcat tccagggccg
     8821 cccggcgaga aacacgaccg cgatagtggc agccgaagca agtgcgacct gtattgcccc
     8881 tcccttcagg acaaggccct tgatctccca gagctggcgg aacgagaact caaggccgat
     8941 tgaaaagagg aggaggatga tgccgatctc ggcgagtacg tcgacctggg ccgggctgtg
     9001 gatcagggag aggaccgagg ggccggctat ggcgcccgcg acgatgtacc cgatcatcgg
     9061 aggaatgcgg atccggctga agaggaggcc taccaggagg gaaaggccga atatgaccag
     9121 gatatcatat aatatcgcaa gttccatgaa ccgatctcac tatagtgatg tcatcagggg
     9181 agagggtcgt cccatgaatg catcctcgtt gatatatacg tataaaggaa ggatcccaca
     9241 aaaagcatgc acttggctcc agtgtaccgt gaaggatcac cgcactcttc attctcgatg
     9301 agatgctgtg ttcatttccc cacccaaaaa aactacatga ttctcttcga gacacacgca
     9361 tacgcattca tgaaaatggc gcagccattt tcccaggaaa actatatagg ggatcatcct
     9421 cctcgcgaat ggctgttccc gtggacattt caccggaaaa gtgctttata ttcgccggac
     9481 catatccctg atgcgaagag agatttcgtt tatactattg agcaggagag aaagagacga
     9541 tggttcgtgt gaaggacatc cctatgccca ccaggtgggc aatcgccacc caggcgctca
     9601 cccacctggt gatggccgcg gatccgcagc agtgcaggga taatcccgca tatctgatgg
     9661 atgaactggg agaggagata cgggggattg caggccgcta ccagatgccg agggaggatg
     9721 caggggggct cgtgcagacg ctcggggcaa tctcggtgat cctctttggt cccacattcg
     9781 aaaccccgta catcgagggg ttccacgagg agtcggtgat acggctctca gagtgtgcga
     9841 tgtttcgtcc tgaacgggag aagaagaggg acccgtcaca cgtgaaccag gtgtgcaccg
     9901 cgtacgtgat gagcgcaata cgtgccctga accccgaata taccatcagg atctcgcgtg
     9961 cacggtgccg gggcgattcc ttctgcgaga tgatcatcga gaagaagaag ccttgatttt
    10021 ctgccggcac atggcaggag gacacatccg ggggacgaac cattccagga tggcaaccga
    10081 acacgcgaaa attgggtgac attccatgaa aaaggaacga gagcacatca accaccccgt
    10141 tcccccctgg cacgccctcc ctatcgagca ggtatacgag acgctcatct cggacgggcg
    10201 cgggctctcg cgggaggaag ctgcccggcg gcttgtgcag tatgggaaga attctctgcc
    10261 tgcccgggaa gcgccgggaa tcgctgagat cgtgctccac cagttcaaga gcccgctcat
    10321 ctatatcctc cttgcggcag ggatcatctc gatcctgata ggggatgtca aggatgcagg
    10381 gttcatattc ctggtggtga tcatcaatgc cgccattggc actgcacagg agtggaaggc
    10441 cgagcagagt gcaatgaaac tccagagcct gctccaggtg atgtcccggg tcatgagggg
    10501 agggacctgg cagatgcttc cggcagagga cctggtgatt ggggacgtcg tgcagctcga
    10561 atcagggatg cgggtcccgg cagatctccg actcctccac gcggcgaacc tcgccattga
    10621 cgaatcgctc cttacaggag aatcagtggc agtcgaaaag gcagttggtg tgcgcgatga
    10681 gaacatccct gtgagcgacc ggtccaacat ggcgtatgcc ggcagcaccg tggtcaccgg
    10741 aaggggatgc gggatagtca ccggcacggc caccaggacc gaggtaggca tgatcgccga
    10801 ggcagtttcg gggacagagt ctgcaaaacc tcccctgatc atccgcatgg aagccttctc
    10861 ccagaaggtg gggattctca tcatcgccgc aagcatcgtc atggcgatcg tagccctgtc
    10921 ccgcggaact ccgcccatag aggtcttctt cctcgcggtt gccctcgtgg tgtcggccat
    10981 ccccgagggg ttgccggttg ctatcactgt cgccctctca atcgcagcct cgaggatggc
    11041 gcaaagacac gtcatcgtca gaaagcttgc tgccgtcgag agcctcggaa gctgcacgac
    11101 aattgccact gacaagaccg ggacactgac tgtaaaccag cagactgccc gggttctttt
    11161 tattgctcca ggggcgtatg ccgaggcgga tggtgtcggg tacgaccccg gtgacggccg
    11221 catcaccgcg gagggagtcc ccctgcaggg accgctctcg gatatagtgc tccggctcgc
    11281 aagggccgcg gtcatctgca acgagggggc gctctaccgc gaggacggca catgggtcca
    11341 ccacggggat gcgatggacg tggcgttcct tactctcggg tacaagacag gccttgagcc
    11401 tgacgccgtc aggaatgcga tccgaacggt ggcaaccgtc cccttcgaat ctgaacggat
    11461 gtatgcggca gtctactaca gggatgacga cggcacccag ggaggaaagg tgaagattgc
    11521 ggtgaaaggg gcactggaag cggtcctgcc atactgcacc tcaatggcca ctcccgcagg
    11581 cgtagttccc ctggattcgg ctggagttga ggagcacctc atcaggatga tggaggatgg
    11641 ctaccgggtg cttgcggtgg cagaaggcga gttatccgag ccgcccgggc agaatgcgga
    11701 gctctccgat gtcaggccca gactcaccct tctcggcctc gcgggattca tcgatcccct
    11761 gcggccggac gtgaaggact ctgtcaggct tgcccaccat gcagggatcg atgtggtcat
    11821 gatcaccgga gaccacccga ggaccgcgtt tgccattgcg cgcgagctcg ggatcgccca
    11881 gtcgttttct gaggtgatga ccgggcagca gcttggcgcg atgggagatc caaatctccc
    11941 ggcattcgcc gaagcgatcg acagggtcag ggtatttgca cgcgtaaccc ctgtgcagaa
    12001 gatggagatc gtcgacaccc tcgtcaggag ggggcatttc gttgcagtga ccggcgacgg
    12061 ggtgaacgac gcccctgcac tcaggcgggc caacatcggt gtcgccatgg ggtctggtac
    12121 cgatgtggca aaggacacat cgtcgatgat cgtgaccgat gacaatttct cgtcaatcgt
    12181 tgccggagtc gaggaggggc ggtttgccta cgacaacatc cgcaaggtga cgtatctcct
    12241 catctcgacc gggctctccg aggtggtgct tttcatcctt gcccttattg cagggctgcc
    12301 tctcccgctc cttgcagtcc agctgctctg gctgaacctc gtcacgaacg gtatccaggg
    12361 tgtcgctctt gcatttgagg caggggaaaa ggggacgatg cggaggaagc cccgggaccc
    12421 gaaagaaggg atattcaacg gcctgatggt aaaggagacg gcgctctcgg ggatgacgat
    12481 aggcgtcgtt gcattcctct tcttcgggtg gatgatcgga agcggtgtcg aggagtcgat
    12541 agcgcgaaac ctcctcctgc tgttgatggt gctcttcgag aacttccatg tctttaactg
    12601 ccggtccgag tggcgctccc tcttcagggt gccgctccgg aacaacgcct tcctcgtgat
    12661 tggggttatc ctgatgcagg gcctgcacat cctctccatg aacctcccat tcatgcagga
    12721 cctgctcagc ctctcgcctg tgacgcttga ccagtggctg ctctgccttg tcatcgcggc
    12781 aagtgtcgtt gtggtgatgg agatcttcaa gtacgtaagc catcggagca tgccagatgc
    12841 agcatctcgc ggaggggttg cagtatgaga gcgagggcaa cagtcccgtt ccatccccgg
    12901 ggtgggtacg taagagctgc agccccgcgc cttcaaatcc ctcttttcag ggggacatgc
    12961 actgaatgac gccagagcgg gagcactggt cctcgggtgc gggctttgtc ctggcagcca
    13021 ttggatccgc ggtcggtatt gggaatatct ggcggttctc agcggtggtg ggacagaacg
    13081 ggggcggtgc atacctcgtc ccgtatctca ttgcggtatt cgcgttcgcg atgcccctga
    13141 tgatactcga acttgccatg gggcggcatt tccagggcac ggtcgtgagc gcgttttgtt
    13201 ccgtgagggc ggagttccgc gtgcttggat ggctcctctg cgcaattatc ttcctcgtgc
    13261 tcagctatta ccttgtcatc gccgggtgga cggcggcata cgcactcttc tccctttccg
    13321 gagagactgt gccgttcgcc tcgttcactg gcggcgtcct ccctgtcctc ttcttcctct
    13381 tcacagcggt catcacggca gcggttatct cgaaggggat ccagaagggg atcgagcgtc
    13441 tttccgtagt cctcattccc atatcactca gcatcctcgt gatcctcgcg ctctataatg
    13501 ccacccttcc cgggttcgcg gagggcatat tattcctgtt caccccggac
//
LOCUS       DAOQYR010000062        13238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_5196_length_13238_cov_9.475916, whole genome shotgun sequence.
ACCESSION   DAOQYR010000062 DAOQYR010000000
VERSION     DAOQYR010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 13238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13238
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5196_length_13238_cov_9.475916"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..919)
                     /locus_tag="PKM87_08080"
     CDS             complement(149..919)
                     /locus_tag="PKM87_08080"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ30061.1"
                     /translation="MGIPAPTGSTLAAYLNGQERGRITTHAPGQYGSTCIFGKRLKVQ
                     PTEEEYTGDAILLIEFYVNGLKADQTSVFHPGAIQSLDLTVSRVPTPEPTPFEEPEPV
                     VALNATPRCGYAPLLVAFADLTSPGAESWLWDFGDGYLSHEQHPSHLYRHTGNYTVNL
                     TVCTVSGCSGLQVPDYIQVIKGTMYVFPGQTNLPKDPDNDGFYEDLNGDGVIRMDDLF
                     LFFSYREWIGENMAENPFDYNLNGRLDLNDIFSLFIEL"
     gene            1302..1856
                     /locus_tag="PKM87_08085"
     CDS             1302..1856
                     /locus_tag="PKM87_08085"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HNQ30062.1"
                     /translation="MVVPSRVFFTKGVGVHKDRLASFELALRKAGIEKFNLVYVSSIF
                     PPNCKMVSVEEGTKMLEPGEITYCVMAKNDTNEPNRLISAAIGLALPKEAREYGYLSE
                     HHAYGETAEKTGEYAEDLAATMLATTLGIEFDINQAWQEREQIYKASGKIIRTTQVCQ
                     SAQGDKNGMWTTVIAAAVFVTNRT"
     gene            complement(1853..2335)
                     /locus_tag="PKM87_08090"
     CDS             complement(1853..2335)
                     /locus_tag="PKM87_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNQ30063.1"
                     /translation="MPGPDEQPVIVGIGEFHAGKNPMSSIGLGSCIGLVIFDREKPIG
                     ALAHIMLPDSQGRAERPAKYADTAVIHLVKELNKLGCRTPSLAAKICGGASMFQAFSG
                     NLNIGERNVEAVRVHLKKLSLSIVAEEVGGTVGRTIVYNPAENGKISVKTADGTRKII
                     "
     gene            complement(2351..2956)
                     /locus_tag="PKM87_08095"
     CDS             complement(2351..2956)
                     /locus_tag="PKM87_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HNQ30064.1"
                     /translation="MLNSHQRDALRELGNIGAAHAATTLSTMLMSNIEMEVPEISVVD
                     IAKIHEHVGDELSALVIFQITGEVSGGGYLLLHIPKNSVIRLTNAMLGMTDLERDLTE
                     MDESALLEIGNIMVSAFLDATATLLSIIMLPSPPSMIIDMPHAAFQTILAMQEFSEID
                     QVVIFRTELRSDQHKISSNLFLLPNKPMLDEILSMLENLMK"
     gene            complement(2961..4946)
                     /locus_tag="PKM87_08100"
     CDS             complement(2961..4946)
                     /locus_tag="PKM87_08100"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNQ30065.1"
                     /translation="MSELDAYRSLYIAESRENHENIVKNLLVLEGEASSEAIAEIFRS
                     AHSLKGMSASMDFKGMERLCHAMEDVFQEIRNGNMAVTHALMDTLLSASDTMEAMLDD
                     IEAGGPGIPDDLDDLVEGLKKWIGLKCDALPKNDTLKSSDTAEGTQSGELISAPGGNA
                     QGLNNYCITLCLSDQCDNRNLRAMIILQNLEELGRLERVVPDRAIVEDGEFNGRFTVE
                     IASDAGIEAIKTVTSSSDVKEAVVEDLKSPAPSPAVIMKSAESPGEKEEERRSPQRTE
                     KVEKGREVKNIRVDISRLDVMMNLVEDLVINRGRITEIAREHQMKDLDETLNMIGRSV
                     SDLQNLMMNIRMIPLNHIFNRFPRTVRDIANREGKEVEFIIEGGDTELDRSVMDGLND
                     PLLHLIRNAIDHGIERPDVRETAGKPRKGTLLLSARRDRDNVIITIQDDGKGISAERV
                     KRKAIERGLVFEAEAENLSREEIIDFLFRPGFSTADAITDISGRGVGLDVVRTTIESL
                     KGTIKVESTEGAGSRFELLLPPTMAIVTVMMVRINGRRCAIPVHNVVEVASLERECIH
                     SIGGHETILLRDEVLPLDRLDDMFGHSERTDILVVLQYQNRKRSIAVDLIEGQQEVVI
                     KPLSTVVGTCRGVSGVTIPGDGEVVPVLDVNALIKES"
     gene            complement(4950..5978)
                     /gene="cheB"
                     /locus_tag="PKM87_08105"
     CDS             complement(4950..5978)
                     /gene="cheB"
                     /locus_tag="PKM87_08105"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HNQ30066.1"
                     /translation="MIRVLVVDDSLFMRTMIRDMLEKDPDIEVVATAVDGVDALKKIS
                     ELKPDVVTLDIEMPRMNGLEVLKSRSQFRDFPRVMMLSSLTSEGAEMTRKAMLLGADD
                     FMPKPKDMHAIKGIERELLEKIHNLVNIAYIVRPERRKAHLADRVVVVGASAGGPPML
                     DILISSFKASIPAAMVITQHMPEGGFTASLATRLNRISPMPVKETENGDVLNQASIYI
                     SRAGHHSVISALLTDTGEKGGRIVHSHSPPLHSVRPAVDKTFVSASSVFGERVVSVIL
                     SGMGNDGGEGMAVVREHGGRTIVCREEDCLVYGMARSALSKNCVEQVLPLEKIGEEIT
                     RLVAGMAN"
     gene            complement(5983..6345)
                     /locus_tag="PKM87_08110"
     CDS             complement(5983..6345)
                     /locus_tag="PKM87_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ30067.1"
                     /translation="MGRILIVDDTLFMRTLLKNILFSGGHDIVGEAGDGDEAIAKYQE
                     LKPDLVTMDVVMPKVNGIEALKGIKTIDPNAKVVMCTAVGQEQMVKLAIKTGAKGYIV
                     KPFQAPKVLEEIKNVLGS"
     gene            complement(6489..7295)
                     /locus_tag="PKM87_08115"
     CDS             complement(6489..7295)
                     /locus_tag="PKM87_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheF family chemotaxis protein"
                     /protein_id="HNQ30068.1"
                     /translation="MAVVPVKIEKDGKWVVSKIDLQQDRMSIAEPLQKAIPFKSVVDL
                     EQKKNLLIITVKGEPPEVFRIASVDKVLQAMKRLIIMSCSAYRLTAYFMSPAVRGGVM
                     VTNATWEKGAIAVLKTSIWFVSQNKQVSVPLNEVTGIELTKREVQGKQVDVVKIDHLE
                     NSDVVTSYVLCPLSTLQILLNFLQDATKDLETSGEELDPLSAQVGMLIYSGMDSHAIE
                     NMLNISHKELDNIYEKLLKIGLIEVVFVRKEVQLTPKGVRFITDAVKPPT"
     gene            complement(7321..8478)
                     /locus_tag="PKM87_08120"
     CDS             complement(7321..8478)
                     /locus_tag="PKM87_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30069.1"
                     /translation="MKDSTLKLILFAVIIVFGSLMILFDIPVFYLLIGAVVLGILVLI
                     LAGGVKMPSIKRDRAKKEVGTPQDQAKKKAKEKKPPKEKKAKAPGTKSGVGDFFSSLK
                     GAFSVLGRDLGRKKKSKAELETHEKKIDSLLDQSIQGAAVASLDDIIPDAAPAEKKKT
                     SDPFTALVGEDLNADLLNGIQGAEDFSILDDSELGIEAGVEDQSQLSAQGDMSSLDIT
                     LSGDDNPITLDETNDADEVKEILEAHSGELGLDQDAGMSLAEDAMEGLDGLELEGMDL
                     GEEGEGPAPSAAAAPAGKTAATTLASPPGRSEPPKPAAPAFSPEQDMLSFSKGRGQDD
                     DLMASLRSDAKSTKKSEYASLIRDMKDIRVDASDLQSELEGLLKPRRPKPE"
     gene            complement(8480..10078)
                     /gene="flaJ"
                     /locus_tag="PKM87_08125"
     CDS             complement(8480..10078)
                     /gene="flaJ"
                     /locus_tag="PKM87_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaellar assembly protein FlaJ"
                     /protein_id="HNQ30070.1"
                     /translation="MAFAGFGKSVRSANAGKLPFQDLGTSVKSHLATLTENKQMGPDL
                     LFMVTYMASITTAQATRPEIFAYTAGRTEYVTCKYIQRVEFFVKRWNYSYVEGLSIIA
                     EKAKNDMLRSMLHRYGNSIESGVPDEDFLTRELATIRSVFRNAYEQGLEMLKKWSDAY
                     IAMLFSANLIGIIIMLSIAIFAPDDVQQTLRSAYFIVITIAIFGLVTMYKSVPSDDKT
                     HALQRCSKEQAIVRKLEMKVIPVALIAAILLYFISQNAGLSMLFLGFILMPLGVIGYL
                     DDRNIVLRDAEFTTFIRSLGAVMGGKGITVGSALAEIDRKSMENLEPFIAGVYSKINL
                     GLDEKLSWDRFVGESGSNLIYKYLNIFRDAVELGGKPDIIGQIVGSSMLEQVLLREKR
                     NSIAMGFVILLVPMHAMMVGIFLFLYHIMVMMSKAISSVVAELGSSSSALSNPGTVGG
                     SLGGSGLSMFVNFPEQEVGLFVVIMIIIITIANVIAGKIVMGGDRYMYYFFSSLLLLV
                     TGIIYIAAPVIVAMFFNIPTFQGV"
     gene            complement(10079..11947)
                     /locus_tag="PKM87_08130"
     CDS             complement(10079..11947)
                     /locus_tag="PKM87_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HNQ30071.1"
                     /translation="MPALTAALNLPFKPAENTEDNDCVNNIESCALYRMLPVNAKEYV
                     RKSPHLLEYLHIFPVDEYGIPLFFSELKRDLKSMTDPNLIYPADDTVFVHIFFDQNDV
                     RNYYIPVDPSFMHSVKEMLPAVEFKLIDLLDALETDPVTDEERTNVLKTLISQVLYVQ
                     SPGDQVAALGGKVEKQGFSSKIKDFLNKDLTAKTNIHRKSAMANVRQTPDGRIIVNPQ
                     EYRALEYMIIRDKIDVGLLKPFINDRYIEDITVDGVGPIFVEHKIFKGLKSVVGWDNT
                     DELDNFVIKLAERTKRPITYRNPIVDATLPDGSRINIVYSTDISRKGSNFTIRKAMDD
                     PISILKLIEFKTCDYLVAGYLWICIENGMSLFMSGETASGKTTSLNAFTAFIPPENKI
                     VTIEDTPELQIPHKNWTREVSKGKGKGEGEGSDVTMFDLLKAALRQRPNQILVGEIRG
                     VEGAVAFGAMQTGHPVLSTFHAASVEKLIQRLCGDPINIPKTYVDNLNIVVIQSAVKR
                     PSGETVRRILSVNELVGYNPETQGFSFVEMFHWNPATDLHEFPGRGSSYLLENRIATM
                     LGIPEHKKASIYDEVEKRARILERLHKAGYTGFYDLYFMMTKVKKQGLLKIDVDAM"
     gene            complement(11949..12695)
                     /locus_tag="PKM87_08135"
     CDS             complement(11949..12695)
                     /locus_tag="PKM87_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HNQ30072.1"
                     /translation="MVASINDGLSSLLGGEDKNILSTGNNEIDKKIADGIPLGSLTLI
                     EGENDTGKSVLTQQIMWGAMKQGLCVDFYSTELTAKSFLSQMESMSLDVSEYFAWGYI
                     RLFHLHMVGFKWTKEEMDGILQRIVAHIRTSKAQVVVIDSLTMFTEYTQQSSILTFLT
                     SCKNIVDQGKTILITLHTYAFEEDTLIRIRSICDAHLSMKKALIGDKYVMVLEVVKVR
                     GARKTTGNLVSFEVHPGYGMKIIPISMAKV"
     gene            complement(12770..>13238)
                     /locus_tag="PKM87_08140"
     CDS             complement(12770..>13238)
                     /locus_tag="PKM87_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30073.1"
                     /translation="ATALGIVLLLLTAYFLAGGALAAIGVVSDAQRETTALQSKMLGT
                     SASVLDSASDGSLLYFEIMNDGKEPIRDMEGIDVYLNDPTIGWSLYTYDETPGDGHWT
                     KVGSTAGDYTAEKIYTGQWDPGEILNISVSYSGISPNYFKIVTANGVGTLFPV"
BASE COUNT         2771 a         3679 c         3551 g         3237 t
ORIGIN      
        1 gccttcatga tttccatata tggtgtgcgg ggaacgtttt aataagtgtc gggatttcca
       61 gaaattttca aaataaaacc ctacatggtc attattgcat aggtattttc acgcaaaaaa
      121 agctggattg gcagccgccg atacaatttt acagctcaat aaacagggag aagatatcat
      181 tcaaatccag gcggccgttc aggttataat caaaaggatt ttctgccatg ttctccccga
      241 tccattcacg gtacgagaaa aaaaggaaaa ggtcatccat cctgatgacc ccgtcaccat
      301 tcaggtcttc atagaatcca tcgttatccg gatctttggg aaggtttgtc tggcccggaa
      361 atacgtacat cgttcctttt attacctgaa tatagtcagg aacttggagg cctgaacagc
      421 cggaaacggt acataccgtc aggttcacgg tataattccc agtgtgcctg tacaggtgcg
      481 aggggtgctg ttcatgggag agatacccgt caccgaaatc ccacaaccag gattcggcac
      541 cgggggatgt caagtctgcg aaggcaacca ggaggggtgc atacccgcac cggggtgtgg
      601 cattcaatgc cactaccggc tcaggctcct caaagggggt aggttcaggg gtaggaaccc
      661 ggctcacggt gagatccagc gattggatag caccagggtg gaacaccgaa gtctggtctg
      721 ctttcagtcc gttcacgtag aattcaatta aaagaatggc atcccccgta tattcctctt
      781 ctgtcggctg gactttcagt cgtttgccaa aaatgcaggt acttccgtat tgcccgggag
      841 catgcgtcgt gatccggcct ctctcctgcc cattgaggta tgctgccagt gtcgatcccg
      901 tcggagcagg gatccccaaa attgtcacct cgccatagta ttcgcaggga accggggtat
      961 ctgcataaac gggaacaata aatacactcc ccaggctgca taggaataaa atcgctaaaa
     1021 tgactcgttt ttcacgaagc atgacgatac agcctcttcc tattcgtctc ctccctatat
     1081 aagtatatat cctgcttcaa cccgtctggg ttcaggaata ggagtgaatc aaatcgaaaa
     1141 tgaagattca ggttattgga agagcggaaa tctcccgaga aagaatagga gcattccaga
     1201 ttctcttaaa aaatttgaga aatgccatat taatacgaca ttcatagccc aattttgaaa
     1261 ataaaaagtt taaagagttg aaacgcacca gatcttgcca tatggtggtc ccgagcaggg
     1321 tattctttac aaagggggtg ggtgttcaca aagacaggct cgcctccttt gagctggcgc
     1381 ttcggaaggc gggaatcgag aagttcaacc tggtctatgt atcgagcata tttcccccga
     1441 attgtaagat ggtctctgtg gaggagggca cgaagatgct tgagcctggg gagatcactt
     1501 actgcgtgat ggccaagaac gatacaaacg agccgaacag gctgatatcg gcagcaatcg
     1561 ggcttgcgct cccgaaagag gccagggaat acgggtatct ctctgaacac catgcctacg
     1621 gtgagacggc agaaaagaca ggagagtacg cagaagacct tgccgcgacc atgcttgcga
     1681 ccaccctcgg catcgaattc gatatcaacc aggcatggca ggagcgggag cagatctaca
     1741 aggccagcgg caagatcatc aggaccaccc aggtgtgcca gtctgcccag ggtgacaaga
     1801 acgggatgtg gaccacggtc atcgcagccg cagtctttgt gaccaaccgc acctaaatta
     1861 tttttcgcgt cccatcagcc gttttcacgc tgatttttcc gttttctgcg ggattgtaca
     1921 cgattgtcct cccaacggtc ccgccgacct cttctgcaac gatggaaagg ctcagcttct
     1981 tgagatgcac cctgaccgcc tccacgttcc tctcgccgat attcaggtta ccggagaagg
     2041 cctggaacat ggatgcaccg ccgcatatct tcgctgccag cgagggcgtc ctgcacccga
     2101 gtttgttcaa ctcctttacc aggtgaatga ctgcggtatc tgcatatttt gcgggtcgct
     2161 ccgcccgccc ctgcgaatca ggcagcatga tatgggcgag ggcgccgatg ggcttctccc
     2221 tgtcgaatat gacgagcccg atgcaggacc caagcccaat cgaagacatc gggtttttcc
     2281 ctgcgtggaa ctctccaatc cccacaatca ctggttgttc gtcagggcca ggcatcgttt
     2341 ttcctgttct ttatttcatc aggttctcga gcattgagag gatttcgtcg agcatgggct
     2401 tgttcgggag caaaaagagg ttgcttgaga tcttgtgctg gtcgctgcgg agctcggttc
     2461 ggaagatgac gacctggtcg atctcagaaa attcctgcat ggcaaggatt gtctggaacg
     2521 ccgcatgcgg catgtcgatg atcatggacg gcggtgaagg cagcataatg atcgagagga
     2581 gggttgcggt cgcatcaagg aacgccgata ccatgatgtt cccgatctcg aggagcgcgc
     2641 tctcgtccat ctcggtgaga tcgcgctcga gatcggtcat gccgagcatg gcgttggtga
     2701 gccgtatcac cgagttcttt ggaatatgca aaaggaggta tcctcccccc gagacctctc
     2761 ccgtgatctg gaagatcaca agcgccgaga gttcatcccc cacgtgttca tgaatctttg
     2821 cgatatcgac cacgctgatc tcgggaactt ccatctcgat attcgacatc agcatggtgg
     2881 aaagtgtggt cgccgcgtgt gctgcgccga tgtttccgag ttcccgtaat gcgtcacgtt
     2941 ggtggctgtt gagcatacct tcaactctcc ttgattaatg cattcacatc aagcaccggc
     3001 acaacctcgc cgtcacccgg gatggtaacc ccggataccc cgcggcaggt tcccaccacc
     3061 gtggagagtg gcttgatcac tacctcctgc tggccctcga tgaggtcgac ggcaatgctc
     3121 cgcttccggt tctggtactg gagcactacc aggatgtcgg tccgttccga atggccgaac
     3181 atgtcgtcaa ggcggtccag ggggagtacc tcgtccctga gaaggatagt ttcgtgtccc
     3241 ccgatcgaat ggatgcactc tctctcgagg ctcgcaacct ccaccacgtt gtggacgggt
     3301 atcgcgcacc tccggccatt tatccggacc atcatcaccg tgacgatggc catggtgggg
     3361 gggaggagga gctcgaacct cgagcctgca ccctctgttg actctacctt gatcgttccc
     3421 ttcagggact caatcgtggt gcgcaccacg tcgaggccga cccctcttcc gctgatgtcg
     3481 gtgatcgcgt cagcggtgga gaaccccggt cggaacaaga agtcgatgat ctcttcccgg
     3541 gagaggtttt cggcctccgc ctcaaagaca agccccctct cgatggcttt tctcttcact
     3601 ctttctgcac tgatcccctt cccgtcgtcc tggatagtga tgatgacgtt gtcgcggtct
     3661 ctccgggccg aaaggaggag cgtccccttc cttggctttc cagccgtttc acggacatcg
     3721 ggcctctcga tcccgtgatc gatggcattc ctgatcaggt ggagcagtgg atcgttgagg
     3781 ccgtccatga cgctccggtc aagctcggtg tccccaccct cgatgatgaa ctcgacttcc
     3841 tttccttccc gattggcgat gtccctgacg gtccttggga acctattgaa gatgtggttg
     3901 agagggatca tcctgatgtt catcatcagg ttctggagat cggagaccga ccggccgatc
     3961 atgttgaggg tctcgtcgag gtccttcatc tggtgttccc tcgcgatctc ggtgatgcgt
     4021 ccgcggttga tcacaaggtc ctcgacgagg ttcatcatta cgtcgaggcg gctgatgtcg
     4081 actcggatgt tcttgacttc ccgccccttt tcgacctttt cggtcctctg cggggatctt
     4141 ctctcttcct ccttctctcc tggggattcg gcggacttca tgataactgc aggagaggga
     4201 gcagggcttt tcaggtcttc aacgaccgcc tctttcacat ccgaagagga ggtgacagtc
     4261 tttatggcct cgatccctgc gtcgcttgcg atctcgaccg tgaaacggcc gttaaattcc
     4321 ccgtcttcca cgatggcccg gtcggggacc accctctcca gtctcccgag ttcctcgagg
     4381 ttctggagga tgatcatggc acgaaggttc cggttgtcgc actggtcgct caaacagagt
     4441 gtgatgcagt aattattcag tccctgggca ttgcccccag gggcagaaat caattccccg
     4501 ctctgtgtgc cttctgccgt atcggaggat ttgagtgtat catttttggg aagcgcatca
     4561 catttgagtc cgatccactt ctttaaaccc tcgacaaggt catcaaggtc atccgggatg
     4621 cccgggccgc ctgcctcgat atcatcgagc atcgcctcca ttgtatcgga tgcggagagg
     4681 agggtgtcca tcagggcgtg ggtgactgcc atgttcccgt tccgtatctc ctggaagaca
     4741 tcttccattg catggcaaag ccgctccatc cccttgaagt ccatggacgc tgacatgccc
     4801 ttgagagagt gggcagaacg gaatatctcg gcgattgcct cacttgaggc ctcgccttcc
     4861 agcacaagaa ggttcttcac gatattctcg tggttctccc gtgattcagc gatgtataag
     4921 gacctgtatg catcaagttc agacatgctt cagttcgcca tccccgctac caggcgtgtg
     4981 atctcttccc cgattttttc caggggcagc acctgctcca cgcagttctt cgagagcgcg
     5041 gaacgtgcca tcccatacac aagacagtcc tcctcgcggc acacgatagt ccggccgcca
     5101 tgctcacgca ccactgccat cccttcaccc ccatcgttcc ccattcctga aaggatcacc
     5161 gagacgactc tctcgccaaa cacgctggat gccgagacga acgtcttgtc aacggcagga
     5221 cgaacgctgt gaaggggagg ggaatgcgag tggacgatcc ttcccccttt ctcacccgta
     5281 tctgtcagga gcgccgatat gacggaatgg tgccctgccc tcgagatgta gatactcgcc
     5341 tggttgagca catctccatt ctcagtttcc ttgacaggca tcggagagat gcggttcagg
     5401 cgggttgcaa gggaggcggt gaaccctccc tcgggcatgt gctgggtgat caccattgct
     5461 gcagggatac tggccttgaa cgaagatatg aggatatcga gcatgggcgg gcctcctgcc
     5521 gacgcaccca ccaccacgac cctgtctgcg agatgcgcct ttctcctctc ggggcggacg
     5581 atatacgcga tattgaccag attgtgtatc ttttcgagga gttcgcgctc gatgcccttg
     5641 atcgcatgca tatcctttgg tttcggcatg aaatcgtccg caccgagcag catggctttc
     5701 ctggtcatct ctgcgccttc ggatgtgagg gatgagagca tcatcacccg cggaaaatcc
     5761 ctaaattgcg acctgctctt caatacttcg agcccgttca tcctgggcat ctcgatgtca
     5821 agggtgacga cgtcaggctt cagctcggag atcttcttga gtgcgtccac cccgtctacc
     5881 gctgttgcca ctacctcgat atcgggatct ttctcaagca tgtccctgat catggtgcgc
     5941 atgaagaggg agtcgtccac cacgagaacc ctgatcatct tctcatgaac caagcacgtt
     6001 cttgatctcc tccagcactt ttggggcctg gaatggtttc acgatgtagc ccttcgcgcc
     6061 agtcttgatc gcaagtttta ccatctgctc ctggcctacc gccgtgcaca tgaccacttt
     6121 cgcgttcggg tcgatggtct tgatcccttt cagggcctcg atcccgttca ccttcggcat
     6181 tacgacgtcc attgtcacca ggtccggctt gagctcctgg tacttggcaa tggcctcgtc
     6241 gccgtcaccc gcctctccta cgatgtcatg gccgccggaa aagaggatat tcttgagaag
     6301 ggtacgcatg aagagggtgt cgtccactat gagaattctc cccatatcta tcactataca
     6361 ggtaaatgca aagaatttaa atagttaatc gtggaggccc tatcagactg gtggcatatc
     6421 caaaaaaatc cgcccgcttc ttggcattcc tggttcactt caccccgaaa tgcaaatatc
     6481 ggcagttcct aggttggggg ttttacggca tctgtgatga aacggacccc cttcggggtc
     6541 agctggacct cttttcttac aaatactacc tctattaaac cgatctttaa aagtttctcg
     6601 taaatattgt cgagttcctt gtgggagatg ttcagcatgt tctctattgc gtgcgagtcc
     6661 atcccgctgt agatgagcat gcccacctgg gcagagaggg ggtcgagttc ctccccgctt
     6721 gtctcgaggt cctttgtggc atcctgaagg aaattgagca ggatctgcag ggtggagagt
     6781 gggcagagca cgtaacttgt cactacatcg gaattttcga ggtggtcgat ctttaccaca
     6841 tccacctgct tgccctgcac ctcccgcttg gtcagctcga tcccggtaac ctcgttgagc
     6901 ggaaccgaca cctgcttgtt ctggctcacg aaccagatgc tggtcttgag gactgcgatg
     6961 gctcccttct cccaggtggc gttcgtcacc atcacgccac ccctgaccgc aggagacatg
     7021 aaatatgcag tcaggcggta ggcgctgcag gacatgatga tgagccgctt cattgcctgg
     7081 aggaccttat cgacactcgc gatccggaaa acctccgggg gctctccctt gacggtgatg
     7141 atcaacaggt tctttttctg ctccaggtcc acgacagact tgaatgggat ggccttctgg
     7201 aggggctcag cgatggacat cctgtcctgc tggaggtcga tcttgctgac cacccatttg
     7261 ccatccttct cgatctttac ggggaccact gccatgcttc ctttcacctg tctgaaaaag
     7321 ttattctggt tttgggcgcc ttggtttcag gaggccctcg agctcgctct ggagatcaga
     7381 tgcatcaacc ctgatatctt tcatatccct gataaggctt gcatattcac tcttcttcgt
     7441 ggattttgca tctgaacgga gcgacgccat gaggtcgtcg tcctggcccc tccccttgga
     7501 gaacgagagc atgtcctgtt ccggtgagaa tgcgggggca gcgggttttg gaggttcgga
     7561 tctccccggc ggcgatgcga gcgttgttgc tgccgttttt cccgcgggag cggctgctgc
     7621 cgagggtgca gggccctctc cctcttctcc aaggtccatc ccctccagtt caagaccgtc
     7681 aagtccctcc attgcatcct cggcaaggct catcccggca tcctggtcaa gtccaagttc
     7741 gccgctatgt gcctcgagga tctccttcac ctcgtctgca tcgtttgtct cgtccagggt
     7801 gatggggtta tcgtctccgg agagggtgat atcgagggaa gacatgtctc cctgcgcgga
     7861 aagctgcgac tggtcctcaa cccctgcctc gattccaagc tcgctgtcgt cgaggatgga
     7921 gaagtcttcc gctccctgga taccgttgag aaggtccgcg ttgaggtctt ctcccacgag
     7981 tgcagtgaac gggtcggagg tcttcttctt ctctgccggc gcggcatcgg gaatgatatc
     8041 atcgagcgat gccactgccg ctccctggat ggactggtca aggagggagt cgatcttttt
     8101 ctcgtgcgtc tccagctccg cttttgactt ctttttccta cccaggtctc gcccgaggac
     8161 cgagaacgca cccttgaggg atgagaaaaa atcgccgaca ccgctcttcg tgccgggtgc
     8221 ttttgccttc ttctcttttg ggggcttctt ctccttcgcc ttctttttcg cctgatcctg
     8281 cggggttccc acctctttct tcgcgcgatc tcttttgatt gaaggcatct tcaccccccc
     8341 ggcaaggatc agcacaagta ttccaaggac caccgcaccg atcaggaggt agaagacagg
     8401 gatgtcgaag aggatcatga gtgaaccaaa tacgatgatt accgcgaaaa gaatgagctt
     8461 gagggttgaa tccttcattt cagactcctt ggaaggtggg aatattgaag aacatagcaa
     8521 ctattaccgg cgcggctata taaatgatgc ccgtgacgag gaggagaagg ctgctgaaga
     8581 aataatacat gtaacggtct ccccccatca cgatcttccc cgcaatcaca ttcgcgatgg
     8641 tgatgatgat gatcatgatc accacaaaga ggcccacttc ttgttcaggg aagttcacga
     8701 acatggagag tccgctcccc ccaagcgatc cgcccacggt cccggggttg ctcagtgccg
     8761 aggaggagga cccaagctcc gcgaccacgg aggagatggc ctttgacatc atcaccatga
     8821 tatgatacag gaagaggaaa attcccacca tcatcgcgtg catggggaca agaagtatga
     8881 caaaccccat tgcaatagag ttccgcttct cccggaggag gacctgttcc agcatggagg
     8941 atccgacgat ctgcccgatg atatcgggct ttcctccgag ttccaccgcg tccctgaaga
     9001 tattgaggta cttgtagatg aggttgcttc cggactcccc cacgaacctg tcccagctga
     9061 gtttctcgtc aaggccaagg ttgatctttg agtatacccc ggcgatgaag ggttcgaggt
     9121 tctccatcga tttccggtcg atctcggcga gggcgctccc tacggtaatt ccctttcccc
     9181 ccatgaccgc tcccaggctc ctgatgaacg tggtgaactc ggcatcgcgc aggacaatgt
     9241 tcctgtcatc gaggtaccct atcacgccaa ggggcatgag gatgaatccg aggaagagca
     9301 tcgagagccc cgcgttctgg gagatgaaat agaggagtat tgcagcgatc aacgcgacag
     9361 ggatcacttt catctccaat ttccgcacga tggcctgctc ctttgagcac cgctgcaggg
     9421 catgggtctt gtcgtcggat gggacggact tgtacatggt gacgaggccg aagatcgcga
     9481 tagtgatcac gatgaaatag gcggacctga gcgtctgctg gacatcatcg ggcgcaaaga
     9541 tcgcaatcga gagcataatg atgatgccga tgaggttggc ggagaagagc atggcaatgt
     9601 aggcatcgct ccatttcttc agcatctcca ggccctgctc ataggcattc ctaaagacgc
     9661 tccggatcgt cgcaagttcc cgggtgagga agtcctcgtc ggggacgccg gactctatgg
     9721 aatttccgta gcggtggagc atgctccgca gcatgtcgtt cttcgctttc tcggcgatga
     9781 tcgagagacc ctcgacgtaa ctgtaattcc agcgcttcac aaagaattca acgcgctgga
     9841 tatacttgca cgtgacatac tccgtacgtc ccgcggtata tgcgaaaatt tcaggcctgg
     9901 tcgcctgggc ggtcgtgatg gaggccatgt aggtgaccat gaaaaggagg tccggcccca
     9961 tctgcttgtt ctcagtgagg gtggccaggt gactctttac tgatgtgcca aggtcctgaa
    10021 acggaagctt tcccgcattc gccgatctga ctgactttcc gaaccctgca aacgccatct
    10081 acatcgcatc gacgtcgatc ttcaggagtc cctgcttctt cacctttgtc atcatgaaat
    10141 acaggtcgta gaatcccgta tatcctgcct tgtgcagccg ctcgaggatc ctcgccctct
    10201 tctcgacctc gtcgtagatt gacgctttct tatgttcggg tatgccgagc atcgtggcga
    10261 tcctgttttc aaggagatag ctgctccctc ggcccggaaa ttcgtgcaaa tcggtcgcag
    10321 ggttccagtg aaacatctct acgaacgaga acccctgtgt ctcggggtta tagccgacaa
    10381 gttcattcac gctcaggatc cgccggactg tctctccgct gggacgcttc actgcgctct
    10441 ggatgaccac gatgttgagg ttgtcgacgt aggtctttgg gatgttgatc gggtctccgc
    10501 agagacgctg gatgagtttc tcgacagatg cggcgtggaa cgtagagagg accgggtgac
    10561 ctgtctgcat cgcaccgaat gcgaccgccc cttcgacacc acggatctcg cccaccagga
    10621 tctggttcgg ccgctggcgg agcgcggcct tgaggaggtc aaacatggtg acatcagagc
    10681 cttctccttc acccttcccc ttccccttcg agacctcgcg cgtccagttc ttgtgcggga
    10741 tctggagctc aggggtatcc tcaatcgtca cgatcttatt ttcgggcggg atgaatgcgg
    10801 tgaaggcatt gaggctggtg gtctttccgc ttgccgtctc tccgctcatg aagagcgaca
    10861 tcccgttctc gatgcatatc cagagatagc ccgcgaccag gtagtcacag gtcttgaact
    10921 cgatcagctt caggatgctg atggggtcgt ccatggcctt acggatggtg aagttactcc
    10981 ccttcctgct gatgtcggtg ctatacacga tattgatacg tgacccatca ggaagagtgg
    11041 catctacgat tgggttccgg taggtgatcg gtctcttggt ccgctctgcg agcttgatca
    11101 cgaagttatc gagttcatcg gtgttgtccc agcccaccac cgacttgagc cccttgaaga
    11161 tcttgtgctc gacaaagatg ggcccaaccc cgtccaccgt gatatcctcg atgtacctgt
    11221 cattgatgaa gggtttcagg agcccgacat ctatcttgtc gcggatgatc atgtactcga
    11281 gcgcccggta ttcctgcgga ttcacgatga tccgtccgtc cggggtctgg cggacgtttg
    11341 ccatcgccga tttccggtgg atattcgtct ttgccgtcag gtccttgttc aaaaagtcct
    11401 tgatcttgct cgaaaacccc tgcttctcga ccttcccgcc gagtgcagcg acctggtcac
    11461 cggggctctg tacgtagagc acctgggaga tcagggtctt taacacgttc gtccgctctt
    11521 catcagtaac cgggtccgtc tcaagggcgt ccagcaggtc gatcagcttg aactccaccg
    11581 cggggagcat ctcctttacg ctatgcatga acgagggatc gacgggaatg tagtagttcc
    11641 ggacgtcgtt ttggtcaaag aagatgtgga caaagaccgt gtcgtcagca ggatagataa
    11701 ggttcgggtc ggtcatcgat ttcaggtccc gcttcagttc agagaagaag agggggatcc
    11761 cgtactcatc gaccgggaag atgtgcaggt actcgagaag gtgcgggctc ttccttacgt
    11821 actcctttgc gttcactggg agcatgcggt agagcgcgca gctctcgatg ttgttgacac
    11881 agtcattgtc ctccgtgttc tctgccggct tgaatggaag gttgagggcg gcggtgagcg
    11941 cgggcatctc acaccttggc catcgagatc gggatgatct tcatgccgta cccggggtgc
    12001 acctcgaagg ataccaggtt cccggtggtc ttcctcgcgc ccctgacctt gaccacctcg
    12061 agcaccatca cgtacttgtc gccgatgagc gccttcttca tcgagaggtg ggcgtcgcag
    12121 atggaccgga tccttatcag ggtatcctcc tcgaatgcgt aggtatgcag ggtgatgagg
    12181 atcgtctttc cctggtcaac gatgttcttg caactggtca gaaaggtgag gatcgacgac
    12241 tgctgcgtat actcggtgaa catggtgagg gagtcgatca ccacaacctg agccttgctc
    12301 gtccttatat gggcgacaat cctctgcagg atgccgtcca tctcctcctt cgtccacttg
    12361 aacccgacca tgtggaggtg gaagaggcgg atgtagcccc aggcaaagta ttcagagaca
    12421 tcaaggctca tggactccat ctgcgagaga aagctctttg cagtcagctc agtcgagtaa
    12481 aagtcgacgc acagaccctg cttcattgcc ccccacataa tctgctgggt cagcacgctc
    12541 ttcccggtgt cgttctctcc ctcgatcagg gtgagcgagc caagcggtat cccgtcggcg
    12601 atcttcttgt cgatctcgtt gttcccggtc gagaggatgt tcttgtcctc gccgccgagg
    12661 agactggaga gcccgtcgtt gatggatgcc accataataa gtactgatta ttatatcgga
    12721 gtgttgatat aaagtgttcc ttaccggatg taagtgtggg aaaatcttgt caaacaggga
    12781 acagggttcc caccccgttg gcagtcacta tcttgaaata atttggagaa atgcctgaat
    12841 aggagactga aatgttcagg atctcaccgg gatcccattg tccggtgtag atcttttccg
    12901 cagtatagtc ccctgcagtg cttcctactt tggtccagtg cccatctcca ggcgtttcat
    12961 cataggtata aagtgaccag ccgattgtcg gatcattaag atagacgtca atgccttcca
    13021 tatcccgaat cggttccttc ccgtcgttca taatctcaaa ataaagcagt gacccgtcag
    13081 aggcgctatc aaggacgctt gccgaagtgc ccagcatctt tgactggaga gcagttgtct
    13141 ctctttgtgc atcggatact accccgatgg ctgcgagtgc cccacctgca aggaaatatg
    13201 cggtgagtaa taaaagcaca ataccgaggg cggttgca
//
LOCUS       DAOQYR010000063        13121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_5239_length_13121_cov_9.618016, whole genome shotgun sequence.
ACCESSION   DAOQYR010000063 DAOQYR010000000
VERSION     DAOQYR010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 13121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13121
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5239_length_13121_cov_9.618016"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..89
                     /locus_tag="PKM87_08145"
     CDS             <1..89
                     /locus_tag="PKM87_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit RpoH/Rpb5
                     C-terminal domain-containing protein"
                     /protein_id="HNQ30074.1"
                     /translation="GDVIRIVRTSHTAGRAESYRLVIKRPKK"
     gene            110..1669
                     /locus_tag="PKM87_08150"
     CDS             110..1669
                     /locus_tag="PKM87_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B''"
                     /protein_id="HNQ30075.1"
                     /translation="MIDRSVLSKAYFSQEHVARHQLDSFNNFLLQNLQKVVNEQRVIE
                     TDIEIRGRSNDPVWVELGRVEVKKPLVREADGSQGDLFPSEARLRNLTYAAPIHLELT
                     LVQGGERQEPVVTTIGQLPIMVGSAACNLSGMSQEERVSHGEDPLDPGGYFIVNGTER
                     VLMTLEDLASNKIMTEYTERYNERIYVAKVFSQYRGYRALVVVERNRKNLLEVSFPSV
                     AGHLKFVDLMRSLGMVSDHDVVSAVSTDEDILTYMMQNLEESDCDSVEAGVMYVGKKL
                     APNQTKDYQRKRAEFVLDNYLLPHLNYLMPTNLKEGEEGYSEAVAEVRLAKAHFLGRM
                     AEACFDLVLSKRRIDDKDHYSNKRLKLAGDLMEDLFRISLNRLTRDIKYQLERASMRH
                     RDLSIGTAVRADVLTERLLHPLATGNWVGGRTGVSQLLDRVDHMSVLSHLRRVISPLS
                     RSQPHFEARDLHPTQWGRICPSETPEGPNCGLVKNFAQMVEISRGVQDEDEVIRILYS
                     LGVEHIKGDVR"
     gene            1666..3480
                     /gene="rpoB"
                     /locus_tag="PKM87_08155"
     CDS             1666..3480
                     /gene="rpoB"
                     /locus_tag="PKM87_08155"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B"
                     /protein_id="HNQ30076.1"
                     /translation="MKKARVFVDGALIGLVEDPKAMVSQVRAMRRQGVIPTEVNISHK
                     EFNNDVIIHTDRGRARRPLIVLHQGKPLISEEEVEKLRKKEVEFDVFIKKGLIELIDA
                     EEEEDLLIAINPGDATPLHTHMEIDPSLILGIGAAHVPFPEHNASPRVTMGAGMVKQA
                     LGFGAANMKIRPDTRGHLLHYVQKPLVHTQTSDLIGSDDRPAGQNFVVAILSFEGYNI
                     EDALIFNKASIDRGLGRSHFFRTYEGEERRYPGGQVDRIEIPDEEVTGAHGAESYKNL
                     DDDGVINPETVVNEKDVLIGKTSPPRFLEEPTSDLITVEKRRDTSVTMRSNERGIVDT
                     VIITEGENSSRLVKVRTRDQRIPEVGDKFASRHGQKGVIGLITPQEDMPYTESGLTPD
                     LVINPHAIPSRMTIGHMLEMIGGKVGSLEGRRINGTAFSGEREEDLRASLKRQGFAHT
                     GREVMYDGITGKRFSADIYMGVIYYQKLYHMVSSKMHARSRGPVQVLTRQPTEGRARE
                     GGLRFGEMERDVMIGHGAAMALKERLLDESDKVQEYVCARCGMVAMLDRKANTTRCLA
                     CGGETDIYPVEMSYAFKLLLDEMKSMGIAPRLKLEDVV"
     gene            3492..6131
                     /locus_tag="PKM87_08160"
     CDS             3492..6131
                     /locus_tag="PKM87_08160"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HNQ30077.1"
                     /translation="MPSPKRIGKIEFGLLSPKEIRTMSVRKIIWADTYDDDGFPYPQG
                     LMDLNLGVIDPGLRCKTCDQKASECPGHFGHIELAKPVIHVGYTRLIRKLLRVTCRSC
                     SRLLLSPEEIEKVVGTEDDLTGDFLSEKDIKKERLCPHCGEQQLKINFEKPTTFSEIF
                     VDDGKKIEHKLTPADIRARMEKIPDEDLRHLGINPEVARPEWTILTVLPVPPVTMRPS
                     IILENGQRSEDDLTHKLVDIIRINQRFKENQDAGAPQLIIEDLWELLQYHVTTYLDNE
                     VAGCPPARHRSGRPLKTLSQRLKGKDGRFRGSLSGKRVNFSARTVISPDPNLSVIEVG
                     IPLAVANEMTIPVQTNPFNIEELRQMVRYGPFRAGISDPCGANYVIRPDKRRIRLAEG
                     NLETVAEMLEPGWTVERQIRDGDIVLFNRQPSLHRMSIMAHRVKVMDGRTFRLNPAVC
                     PPYNADFDGDEMNLHIPQTEEARAEAAILVAVEENILSPRFGGPIIGGIHDHVSGIFM
                     LTNQLKWHTKEEALYLLKNIGVEHLPAPGMEENGQELWSNKQIFSSVLPKELNMVFRA
                     SSCQNCETCKRQLCERDAYVRIIDGILESGTIDKKAIGAFDGQIVNRIIRQYGMKRAA
                     EFIDAITHLSIRAIMLDGFSFGIDDEDLSKTEYGQIDEVLSNAVNDVQRRIKIYEDGQ
                     LEAMPGRTPEETLEMQIMQVLGKARDRTGEIAGRHLGLGNSAVVMAVSGARGSMLNLT
                     QMAGCVGQQSVRGERIMRGYDDRTLPHFKKGDRGADAHGFITHSYKSGLNPTEFFFHA
                     IGGREGLVDTAVRTSQSGYLQRRMINALQDLKVAYDGTVRTTGGRIIQFCYGEDGTDP
                     MKSSFGDPVDVKGIVESILKEEV"
     gene            6133..7296
                     /gene="rpoA2"
                     /locus_tag="PKM87_08165"
     CDS             6133..7296
                     /gene="rpoA2"
                     /locus_tag="PKM87_08165"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A''"
                     /protein_id="HNQ30078.1"
                     /translation="MDPGFDAAIGKADLPDKTKQQLRTFLDGKEISKEQFSTILDRVI
                     NEYMNTRIEACEAVGIIAAQSIGEPGTQMTMRTFHYAGVAEINVTLGLPRLIEIMDAR
                     KEPSTPTMTIYLQEDYSGDRDRAREVSWQIEAAPLHEFGDIVIDMENMQIVVQLNTAV
                     CDKRKISVAEIVEIAPRKIRDRRHYRDFDHEVDEKGAIMYFMPKDRESYQNLFQLAEH
                     VRNVIVQGIDDIERVVVRKESGEYILYTEGSNLKDVFEVVGVDTTRTRTNNISEISQV
                     LGIEAARNAIIHEALSTLNEQGILVDVRHIMLVADMMCMDGEVKQIGRHGIAGEKESV
                     LSRAAFEVTVNHLLDAAVANEVDELSGVTENVIVGQPIQLGTGDVKLIAKPLN"
     gene            7307..7597
                     /locus_tag="PKM87_08170"
     CDS             7307..7597
                     /locus_tag="PKM87_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30e"
                     /protein_id="HNQ30079.1"
                     /translation="MDFNASLRRAIKTGEVLLGQNMTAQSIENGRAQMVVVAGNCPEN
                     FREMLKSKDNLFVYTFEGSSVQLGKACGKPFMVSALAVINPGESDILNLKRA"
     gene            7602..8048
                     /locus_tag="PKM87_08175"
     CDS             7602..8048
                     /locus_tag="PKM87_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HNQ30080.1"
                     /translation="MAEVLLNEDCMRLISQFEKLTGAGSRDCVIDDRNNRIIFVINPG
                     EMGLAIGKSGASIKKAMDVMGKKIEVVEYANNPEQFLRNCFLPAKVTAVDFEGEGDSQ
                     VAHVEVRDEDRGLAIGKEGKNIFKAKKLAQRQHNISDVLLVNNQAE"
     gene            complement(8146..8397)
                     /locus_tag="PKM87_08180"
     CDS             complement(8146..8397)
                     /locus_tag="PKM87_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30081.1"
                     /translation="MPDRCTHANMVIATTMSEIESAFYDIESIKNDIRDMQYGLTELD
                     GKLHEINQRLRSLQAVLEEICPLPGSSSPPEDPHRSVPL"
     gene            complement(8499..8573)
                     /locus_tag="PKM87_08185"
     tRNA            complement(8499..8573)
                     /locus_tag="PKM87_08185"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8536..8538),aa:Arg,seq:tct)
     gene            complement(8770..9162)
                     /locus_tag="PKM87_08190"
     CDS             complement(8770..9162)
                     /locus_tag="PKM87_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF473 domain-containing protein"
                     /protein_id="HNQ30082.1"
                     /translation="MKCFALTGIAPQVIRELQEGKARTLELQSTHNVVTLAGIPAGSF
                     IFMTSVDMEDISPGDSGIIVELLAVSITMKRIMEYSHGLHYEERERMSARIKVKCVGS
                     STVRSVTTEGFIQPITVEVLKSACYHAG"
     gene            complement(9168..9896)
                     /locus_tag="PKM87_08195"
     CDS             complement(9168..9896)
                     /locus_tag="PKM87_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HNQ30083.1"
                     /translation="MSPDPTTDIKIFSRPLSGPGSSVLMGFPGSGLVGSIALQYMVDQ
                     LEFEQIGSINSKYFPALAMMNKGVINVPVRVYEKGNISVIVADIPIHPMICYEVANGV
                     MDWLEPFSIKEVVTIAGIITNEPEKRVFGVASNEEALSRIQDSTIILPVGSISGIASS
                     ILTECKIRNIPAYGLLGETINAPDPRASAAAIEVLNRMYDIQLDVKPLIEQAEEIEST
                     MQKLAEQVKTAETMPKKEQLPMYG"
     gene            9932..10249
                     /locus_tag="PKM87_08200"
     CDS             9932..10249
                     /locus_tag="PKM87_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5611 family protein"
                     /protein_id="HNQ30084.1"
                     /translation="MQEYPVKRGYTKDLQTTMGAKLKDCFNVEPKKVGDHYQITYGAL
                     KVLDVSTGKEKKTLVVDTKSDTTASDEVILDTNRRFRKYLDEVTGYSTKDRVKKAKSV
                     EPE"
     gene            complement(10253..13018)
                     /gene="leuS"
                     /locus_tag="PKM87_08205"
     CDS             complement(10253..13018)
                     /gene="leuS"
                     /locus_tag="PKM87_08205"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNQ30085.1"
                     /translation="MRQLEEAALHAWEHAFEPDPASREKFYLTVAYPYPSGAMHVGHG
                     RTYIVPDVIARFWRMRGRQVLYPMAFHVTGAPVIGISKRIARGDEKAIFLYRDLYKVP
                     QETLEKFTDPLEIVRHFSEEYRRVMTACGLSIDWRRRFTTVDPQYSKFIEWQWLHLHE
                     GGHVNKGAHPVRFCPQCDNPVGDHDLLEGDRAEILKFTTVMFEWKDSLIPTATLRPET
                     IYGVTNLWVNPGVTYVKARVDGTPWIVSREAAAKLALQDHDVIVTEEIPGSSLVDQVV
                     RHPLCGEVPILPAEFVDPEMASGVVMSVPAHAPFDYIALRDLQAKGKYTSIRPIALIK
                     VPGYGDFPAKDAVERAGIRDQNDPRMDALTQEVYSAEFSNGTLFERYGGEPVRQARES
                     VAVLMLEKFGSAVMYEFDTRPVVCRCGGQVFVKILHDQWFLQYSDPAWKDRVSDHLAT
                     MSLVPPEVRTEFERTIGWLKDWACTRRVGLGTRLPWDPDWLIEPLSDSTIYMAYYTIA
                     HHIKKFGAEELTPEVFDYLFLGKGSPAHCDRERLDPIRKEYEYWYPYDYRFSAKDLIS
                     NHLTFQLFHHAAIFPPHRMPRGMVVFGMGLLNGAKMSSSKGNVFLLEDAVKEFGADTV
                     RMFLVGSAEPWQDFDWRNELVNSTKKQIERFWNMVQESLSAGGDETDVDRWLISRLQQ
                     HIEKTTEALERFQTRQALQEAFYAIDSDLKWYRRRLPTGVEGGCAFRELGPIWVRLLA
                     PIIPFTCERLWTDTGGDGLVSFAPWPVANSKKTDPDLEMAEELLLRTLEDIESIRKVL
                     QITPSSLTLCVAPSWKRAIFSTVAAASDRNAVIKEIMKDENMRKRGKEATETTRQCVT
                     LVHRLPPAFVEQYLKGGINEMEIFQNAREFLERECAMPVHVVDAGECGHPRAAAALPL
                     KPAIVME"
BASE COUNT         3048 a         3622 c         3831 g         2620 t
ORIGIN      
        1 tgggggacgt aatccgtatc gtccggacat cccatacggc cggaagggcc gaatcgtacc
       61 ggctggtcat caaaagaccg aaaaaataat tcaggcgggg ctgatcgttc tgattgacag
      121 gagcgtatta tctaaggcat atttttccca ggagcacgtg gcgcgccacc agctcgactc
      181 ttttaacaat tttcttctgc agaacctgca gaaggtggtc aatgaacaac gcgtcatcga
      241 gactgacatc gagattcgcg ggcggagcaa cgacccggtg tgggtagaac tgggcagggt
      301 tgaggtgaag aagcctctcg tgagagaagc ggacggatcc cagggggatc tcttcccaag
      361 cgaggcaagg ctccgcaacc tcacctatgc cgcaccaatt cacctggagc tgacgcttgt
      421 ccaggggggt gaacgccagg agccggtcgt tactaccatt gggcagctgc ccattatggt
      481 aggttcggct gcctgcaacc tctctgggat gagtcaggag gagagggttt cgcacgggga
      541 ggacccgctt gaccccggcg ggtacttcat cgtgaacggg accgagaggg tattgatgac
      601 cctcgaggat ctcgcatcga acaagatcat gaccgaatac acggagcgct acaatgagcg
      661 catttacgtg gcaaaggtct tctcacaata ccggggctac agggcactcg tggtggtgga
      721 acgaaaccgg aagaacctcc tcgaggtctc attcccttcg gttgcgggac acctgaagtt
      781 cgtggacctg atgcgttcac ttgggatggt cagcgaccat gacgtggtga gcgccgtctc
      841 aaccgacgag gacatcctca cgtacatgat gcagaacctc gaggagagcg actgcgacag
      901 tgtcgaggcg ggagtcatgt acgtgggcaa gaaacttgcg cccaaccaga cgaaggatta
      961 ccagaggaag cgtgcggagt tcgtccttga caactacctc ctgccgcacc tgaactacct
     1021 catgcccacg aacttaaaag agggcgaaga ggggtatagt gaagccgttg cagaggtccg
     1081 ccttgcgaag gcccacttcc tggggcgcat ggcagaggca tgtttcgacc tcgtcctctc
     1141 gaagaggagg attgacgaca aggaccatta ctccaacaag cggctgaagc ttgcaggaga
     1201 cctgatggag gatctcttcc gcatctcact caacaggctc acccgcgaca tcaagtacca
     1261 gctcgagagg gcaagcatgc gtcaccgcga cctctctatc gggacggcag tccgtgccga
     1321 cgtgctgacc gaacgccttc tccacccgct tgcgactgga aactgggtgg gtggcaggac
     1381 aggggtatcc cagctgctgg acagggtcga tcacatgagc gtgctctcac acctccggag
     1441 ggttatatcc cccctctccc gctcgcagcc tcacttcgag gcgcgtgacc tgcaccccac
     1501 ccagtggggg cggatctgcc cgagcgagac acccgagggg ccgaactgcg ggcttgtaaa
     1561 gaattttgcc cagatggtag agatcagccg gggggtccag gatgaagatg aggtgattcg
     1621 tatcctctac tctctcggcg tggagcatat caagggggac gtgagatgaa gaaggcacgg
     1681 gtgtttgtgg acggtgccct gatcgggctc gtggaagacc caaaggccat ggtctcacag
     1741 gtgcgggcca tgcggcgcca gggggtcatc cccaccgaag tgaacatctc ccacaaggaa
     1801 ttcaacaacg atgtcatcat tcacacagac aggggaaggg cacgaaggcc actgatcgtt
     1861 ctgcaccagg gcaaaccgct gatctcggaa gaggaagtgg aaaaactcag gaaaaaggag
     1921 gtggagtttg atgtgttcat taaaaaggga ctcatcgagc tgattgatgc ggaagaggaa
     1981 gaagacctcc tgattgcaat caacccgggt gacgcaactc ctctccacac tcacatggag
     2041 atagatccct cccttatcct ggggataggt gccgcgcatg tcccgttccc cgagcacaat
     2101 gcgagcccca gggtcaccat gggtgcaggc atggtcaagc aggcgctggg attcggggct
     2161 gcaaacatga agatccggcc cgatacgagg gggcatctcc tccattacgt ccagaaaccc
     2221 ctcgtccata cccagacttc ggacctcatc ggttcagatg accggcctgc gggccagaac
     2281 ttcgtcgtgg ctattctctc gttcgagggg tacaacatcg aagatgccct catattcaac
     2341 aaggcatcca tcgatcgggg ccttgggcgc tcccacttct tccgcacata cgagggtgag
     2401 gagcggaggt atcccggcgg acaggtcgac cggatcgaga tccccgatga ggaagtgaca
     2461 ggcgcccacg gcgcagagag ctacaagaac ctcgacgatg acggcgtgat caaccccgag
     2521 actgtggtca acgaaaaaga cgtcctcatc gggaagactt ctccgcctcg tttcctcgag
     2581 gagcccacaa gtgacctgat cactgtcgag aagcggcgtg acacttcagt aacgatgcga
     2641 agcaacgagc gcgggattgt tgatacggta atcattacag aaggagagaa cagctcccgc
     2701 ctggtgaagg tccggaccag ggaccagagg atcccagagg ttggcgacaa gttcgcgtcc
     2761 cgccacgggc agaaaggggt tatcggcctg atcacccccc aggaggatat gccgtatacc
     2821 gaatccgggc tcacccccga cctcgtcatc aatccacatg ccatcccaag ccggatgaca
     2881 atcgggcaca tgcttgagat gatcggaggc aaagtgggat cactcgaagg acggaggatc
     2941 aacggaaccg ccttttcagg cgaacgtgaa gaagatttgc gtgcctcgct gaagcgccag
     3001 ggttttgcac acaccgggcg tgaagtcatg tatgacggca tcaccggaaa acggttctcg
     3061 gccgatatct acatgggtgt aatctactac cagaaactct accacatggt ctcctcgaag
     3121 atgcatgccc ggtcaagggg accggtgcag gtgctgaccc gccagccaac cgaagggagg
     3181 gcaagggaag gaggcttgcg tttcggcgag atggagcgtg acgtgatgat tggtcatggt
     3241 gccgccatgg cattgaagga gcgcctcctt gacgagtctg acaaggtgca ggaatacgtc
     3301 tgtgcacggt gcgggatggt ggccatgctc gataggaagg ccaacaccac caggtgcctc
     3361 gcatgcggtg gcgagacgga catctacccg gtggaaatgt cgtatgcgtt caagctcctc
     3421 ctcgacgaga tgaagagcat ggggatcgcc ccaagactca agctcgaaga cgtggtgtag
     3481 gagggagagt aatgcctagc ccaaagagaa ttggaaagat agaattcgga ctcctctccc
     3541 caaaggagat ccgcaccatg agtgtgcgaa agatcatctg ggctgatacc tatgatgatg
     3601 acgggttccc ctacccccag gggctgatgg acctgaacct cggggtgatc gaccctggtc
     3661 tccggtgcaa gacctgtgac cagaaggcga gcgagtgtcc cggccatttc gggcacatcg
     3721 agctcgcaaa gccggtcatt cacgtcggct acacaaggct gatacgaaaa cttctcaggg
     3781 tgacctgcag aagctgcagc cggctcctcc tctcccctga agagatcgag aaggtggtgg
     3841 ggaccgaaga cgacctgaca ggagactttc tctccgagaa agacatcaaa aaggagaggc
     3901 tgtgccccca ctgcggagag caacagctca agattaactt tgagaagccg acgacgttct
     3961 cggagatctt cgtagacgac gggaagaaga tcgagcacaa gctgaccccc gcggatatcc
     4021 gggcccgcat ggagaagata cccgacgaag acctccggca tctcgggatc aatcccgagg
     4081 tcgcaaggcc cgagtggacg attcttacgg tgctcccggt cccgccggtc acgatgcgcc
     4141 cctcgatcat cctcgagaac gggcagcggt ccgaggacga cctcacccac aagctcgtgg
     4201 acatcatcag gatcaaccag cgtttcaagg agaaccagga cgcgggtgcg ccgcaactga
     4261 tcatcgaaga cctctgggag ctcctgcagt accacgtcac cacctacctt gacaacgagg
     4321 tggcagggtg cccgccggca cgccacagga gtggcaggcc cttaaagaca ctctcacaga
     4381 ggctgaaagg aaaagacggg cgtttccgtg gatcgctctc agggaaaagg gtgaacttct
     4441 cggcccgaac ggtcatctct cccgatccga acctgagcgt gatcgaggtg ggaatacccc
     4501 tggcagtcgc aaacgagatg accatccctg tccagacaaa tcccttcaac atcgaggaac
     4561 tgagacagat ggtgaggtac ggcccgttcc gcgctggaat ctccgatccc tgcggtgcca
     4621 actacgtgat tcgccctgac aagaggcgta tccggctggc tgaggggaac ctcgagacag
     4681 ttgccgagat gctcgaaccg ggatggacgg tggagcgcca gatcagggac ggcgatattg
     4741 tcctcttcaa ccggcagccc tcgctgcacc ggatgagcat catggcccat cgtgtcaagg
     4801 tgatggacgg ccgcacattc aggctgaacc cagcggtatg cccgccatac aacgccgact
     4861 tcgatggaga cgagatgaac ctgcacatcc cccagaccga ggaggcgagg gcggaggccg
     4921 caattctcgt tgcggtagag gagaacatcc tctccccgag gttcggcggc cccatcatcg
     4981 gcggaatcca cgaccacgtc tcgggcatct tcatgctcac caaccagctg aagtggcata
     5041 ccaaggagga ggccctctac ctcctcaaga atatcggcgt ggaacatctc cctgcacccg
     5101 gaatggaaga gaacgggcag gaactctgga gcaacaagca gatcttctcc agtgtgctgc
     5161 cgaaggaact gaacatggtg ttccgggcaa gcagctgcca gaactgcgag acctgcaaga
     5221 gacagctttg cgaacgcgat gcttatgtcc ggattatcga cgggattctc gaatcaggga
     5281 ccatcgacaa gaaggccatc ggggcgttcg acgggcagat tgtgaaccgc atcatccggc
     5341 agtacggcat gaagcgggca gccgagttca tcgacgccat cacccacctc tcgatccgtg
     5401 ccatcatgct ggacgggttc tcgttcggca tcgacgacga ggatctctcg aagacagagt
     5461 acggccagat cgatgaggtg ctctcgaatg cagtcaacga tgtgcagcgg cggatcaaga
     5521 tctacgagga cgggcagctc gaggcaatgc ccgggaggac tcccgaggag accctcgaga
     5581 tgcagatcat gcaggtcctc gggaaggctc gtgaccggac tggtgagatt gccggccggc
     5641 accttggtct cggcaacagc gccgtggtca tggcagtgtc aggggcaagg ggctccatgc
     5701 tgaacctcac ccagatggca gggtgcgtcg ggcagcagtc tgtccgtgga gaacgtatca
     5761 tgagagggta cgacgaccgg acgctccccc acttcaaaaa gggcgacagg ggtgcagatg
     5821 cccacggttt catcacccac agctacaagt cagggctgaa ccctacggag ttctttttcc
     5881 atgccatcgg aggccgcgaa ggtcttgtgg acactgcagt caggacatcg cagagcgggt
     5941 acctgcagag gcgcatgatc aacgccctcc aggacctgaa ggtcgcatac gacgggaccg
     6001 tcaggacaac gggagggcgt atcatccagt tctgctatgg tgaggacggg acggacccca
     6061 tgaaaagcag tttcggtgat cctgtggacg tgaaagggat cgtggaaagc atcctgaaag
     6121 aggaggtgtg agatggatcc cgggtttgat gctgcaatcg ggaaggcaga cctgccagac
     6181 aagaccaagc agcagctccg aacattcctt gacggcaagg aaatctcaaa agagcagttc
     6241 tcaaccatcc tcgacagggt gatcaacgag tatatgaaca cacgcatcga ggcctgcgag
     6301 gcagtcggga tcatcgcggc gcagtcgata ggagagcctg gcacgcagat gacgatgcgt
     6361 acgttccact atgccggggt ggctgagatc aacgtgacgc tcggtctgcc caggctgatc
     6421 gagatcatgg atgccaggaa ggaaccgagt acccccacca tgacaatcta tctccaggag
     6481 gattactcag gagatcgcga ccgtgcgcgg gaggtgagct ggcagatcga agccgcgccc
     6541 ctgcacgagt ttggggatat cgtcatcgac atggagaaca tgcagatcgt ggtgcaactc
     6601 aacaccgcgg tctgcgacaa gaggaagatc tccgttgcag agattgttga gatcgcaccg
     6661 cgcaagatcc gggatcggcg ccattaccgg gatttcgacc acgaggtgga cgagaagggc
     6721 gcgatcatgt acttcatgcc aaaggacagg gagagttacc agaacctctt ccagctcgct
     6781 gaacacgtgc gcaacgtcat agtccagggg attgacgata tcgagcgcgt ggtggtaaga
     6841 aaggagagtg gagagtatat cctatatact gagggctcca accttaagga cgtgtttgag
     6901 gttgtcggtg tggataccac gaggacccgt accaacaaca tcagcgagat ctcccaggtg
     6961 ctcggcatcg aagccgccag gaatgccatc atccacgaag ccctctccac cctgaacgag
     7021 caggggatcc tcgtggacgt gcggcatatc atgctggtcg cggacatgat gtgcatggac
     7081 ggggaagtaa agcagattgg ccggcatgga atcgccggcg agaaggagag tgtcctctcc
     7141 cgtgccgcat tcgaggtgac tgtcaatcat ctgctggatg ctgcagttgc caatgaggtg
     7201 gatgaactgt cgggagtgac ggagaacgtg atcgtgggcc agcccattca gcttggaacc
     7261 ggtgacgtca aactgattgc aaaacctttg aactaggaga attataatgg attttaacgc
     7321 ttctttacga agagccataa aaacaggaga agtcctgctc ggccagaaca tgaccgcgca
     7381 gtccatcgag aacggcaggg cccagatggt ggtagtggcc ggtaactgcc cggaaaattt
     7441 cagggaaatg ctcaaatcaa aggataacct cttcgtctac acgttcgaag ggtccagcgt
     7501 gcaactgggg aaggcatgcg gtaaaccctt tatggtaagc gcccttgcag tcatcaaccc
     7561 tggcgagtcc gatatcctta atctcaagag ggcctgaaac catggctgaa gtgcttctga
     7621 acgaggactg catgcgcctt atatcccagt tcgagaaact gacgggggca gggagcaggg
     7681 actgcgtgat tgacgaccgg aacaaccgca ttatcttcgt catcaacccc ggcgagatgg
     7741 ggcttgcaat cggaaagagc ggggccagca taaagaaggc gatggatgtc atgggcaaga
     7801 agatcgaggt ggtggagtac gcgaacaacc cggagcagtt tctcaggaac tgtttcctgc
     7861 ctgccaaggt tactgccgtc gacttcgagg gcgagggaga ttcccaggtt gcgcatgtcg
     7921 aggtcaggga cgaggaccgc ggactcgcaa tcgggaagga aggaaaaaac atcttcaagg
     7981 cgaagaagct tgcccagcgc cagcacaata tcagcgatgt gctgctggtg aacaaccagg
     8041 cagaatagct ctccttcttg tttttttcag cggaaaaata atatcacgcc ccaactcaag
     8101 tttcctcccc gcatggagag gggatttgcg aaaaacccca ttctttcaga gaggtacaga
     8161 cctgtggggg tcctcaggtg gggatgacga acccgggaga ggacagatct cttcgagcac
     8221 agcctggagt gatctcaaac gctggttgat ctcatgcagt tttccgtcca gttcggtgag
     8281 accatactgc atgtccctga tatcgttctt gatgctttcg atgtcataga atgcgctctc
     8341 gatctctgac atggtagtgg cgattaccat gttggcatgg gtacatctgt caggcatgtg
     8401 tggcactcag gatttgtgtc gcgttcattc atgatgaagg caaccattca agcccgggcg
     8461 tctattcagg gcgaaggccc tgcaaaaaag ggactttacg ggcctggggg gattcgaacc
     8521 cccgacatcc gggttagaag cccggcgctc tatcctggct aagccacaag cccactgaag
     8581 tgtgtatact atgagggtcg atggtaatta atcgttcttt cagataacaa gggatagtcc
     8641 tgcgataata tggcctcctg gttccccttt tttgcatgcc gcgctgcaga tgatgacttt
     8701 agcatttaca cacaagaaaa aaaatatcag atgagtcctt tcgcacagtt ccgttacctg
     8761 aacaaggggt tatcccgcat ggtagcaggc cgatttcagc acctcgacgg tgataggctg
     8821 gataaaccct tcggtcgtca cgctccggac cgtcgaggaa cccacgcatt ttaccttgat
     8881 tcgggcagac atccgctccc gttcctcgta atgcagaccg tgcgaatact ccatgatccg
     8941 cttcatcgtg atgctcaccg cgaggagctc gacgatgatt cctgaatctc ccggggagat
     9001 atcctccata tcgacggaag tcatgaatat gaacgatccg gcaggaatac ctgcaagggt
     9061 caccacattg tgcgtactct gtagttccag ggtcctggcc ttgccctcct ggagctctct
     9121 gatcacctgt ggggcgattc cggttaacgc gaagcacttc atgaagatca cccgtacatg
     9181 ggaagctgtt ctttcttcgg catcgtctct gcggtcttca cctgttctgc cagcttctgc
     9241 atggttgact cgatctcttc cgcctgctcg ataagcggct tgacatcgag ttgaatgtca
     9301 tacatcctgt tcaggacctc tatggcggca gctgatgccc gtggatcggg agcattgatc
     9361 gtctctccga gaagaccata cgcagggata ttcctgatct tacactcggt gaggatgctg
     9421 cttgcaatcc ctgaaatgct tccgactggg aggatgatag tggagtcctg tatcctggag
     9481 agtgcctctt cgttgcttgc aactccaaac actctcttct cgggttcgtt ggtgatgatg
     9541 cccgcgatcg tgacgacctc cttgatggag aatggttcca gccagtccat caccccgttc
     9601 gccacctcgt agcagatcat ggggtggatg gggatgtccg cgacgatcac agagatgttc
     9661 cccttctcgt ataccctcac cgggacattg atcacaccct tgttcatcat ggcaagggca
     9721 gggaaatatt tgctgttgat actcccgatc tgctcgaact ccagctggtc caccatgtac
     9781 tggagcgcga tgctccccac aaggccgctc cccggaaatc ccataaggac agatgatccg
     9841 ggaccggaca gtggtcgcga gaagatcttg atatctgtgg ttggatcagg cgacattatc
     9901 ttaacatatt ccatccacct aaaaaagtat tatgcaagaa tatcctgtga aacgagggta
     9961 tacgaaggat ctccagacaa ccatgggggc caagctcaag gactgcttta atgttgaacc
    10021 aaaaaaggtg ggtgatcatt accagatcac gtacggggcc ctgaaagtcc tggacgtctc
    10081 gaccgggaag gagaaaaaga cccttgtagt ggataccaaa tcggatacca ctgcatcaga
    10141 cgaggtcatt ctcgacacca accggcgctt tcgaaaatac ctcgatgagg tgacagggta
    10201 ttccacgaaa gatcgtgtga aaaaggcgaa aagcgtggag cccgaatagg cttcactcca
    10261 tgacgatcgc aggtttcagg gggagggctg cagcagccct ggggtgcccg cactcccccg
    10321 catccacgac atgtaccggc atggcacatt ccctttcgag gaactctctt gcgttctgga
    10381 agatctccat ctcgttgata cctcctttca ggtattgctc gacgaatgca ggaggcaggc
    10441 gatggaccag cgtgacacac tgcctggtcg tctccgtagc ttccttcccg cgttttcgca
    10501 tattctcgtc cttcataatc tcttttatca ctgcgttcct gtctgatgcg gctgccacgg
    10561 ttgagaagat cgccctcttc catgaaggag caacacagag agtaagagag gaaggagtga
    10621 tctggaggac ctttcggatc gactcgatgt cctcgagcgt gcgcaggaga agctcctctg
    10681 ccatttcaag gtcgggatct gtctttttcg aattggcgac cggccagggt gcaaacgaga
    10741 caagcccgtc tccgcccgta tctgtccata acctctcgca ggtgaacggg atgattggtg
    10801 ccaggagcct gacccagatc gggccgagtt ccctgaatgc acagccaccc tcaacccctg
    10861 tcggcaggcg tctcctgtac catttcaggt cagagtcgat ggcatagaat gcttcctgga
    10921 gggcctggcg ggtctggaag cgctcaagtg cttccgttgt cttctcgatg tgctgctgga
    10981 gccgggagat gagccagcgg tccacatcgg tctcgtctcc tcctgcagaa agactctcct
    11041 gcaccatgtt ccagaaccgc tcaatctgtt ttttggtaga attgaccagc tcattgcgcc
    11101 agtcgaagtc ctgccatggt tcggcactcc ccacgaggaa catgcggaca gtgtccgcac
    11161 cgaactcctt gactgcatcc tcgaggagga agacgttccc cttcgacgat gacatcttgg
    11221 caccgttcag gagacccatc ccgaagacca ccatcccccg gggcatgcgg tgtggcggga
    11281 aaatggctgc atgatggaag agctggaacg tcaggtggtt ggagatgagg tctttagccg
    11341 agaaccggta atcgtaggga taccagtatt catactcctt tcttattgga tcgagtctct
    11401 ccctgtcgca gtgagcagga gagccttttc caaggaagag gtaatcgaac acttccggcg
    11461 tgagctcttc ggccccaaac ttcttgatgt ggtgggcgat ggtgtagtat gccatgtaga
    11521 tggtcgagtc gctgagtggc tcgataagcc agtcggggtc ccagggaagc cgggtcccaa
    11581 ggcccacccg ccgggtgcat gcccagtcct tcagccaccc aattgtccgc tcgaactcgg
    11641 ttcgtacctc tggggggaca agggacatcg tggccaggtg atcggagacg cggtccttcc
    11701 aggcaggatc gctgtactgg agaaaccact ggtcatggag gatcttgaca aacacctggc
    11761 cgccgcacct gcagacgacc ggcctcgtat cgaactcgta catcacggcc gatccgaact
    11821 tctcgagcat gaggaccgcg acgctctccc tggcctgcct gacgggctct ccgccgtaac
    11881 gctcaaagag agtgccattt gagaactcgg cgctgtatac ctcctgggtg agggcatcca
    11941 tccttggatc gttctggtcg cggattcctg ccctctctac ggcatccttt gcagggaaat
    12001 ccccgtagcc cgggacctta atcagggcaa tgggcctgat actggtgtac ttcccttttg
    12061 cctggagatc ccgcagcgcg atgtaatcga acggtgcgtg tgcgggaacg ctcatcacga
    12121 cgccgctggc catttctggg tccacaaatt ctgcggggag tatggggacc tcaccgcaga
    12181 gcggatggcg aacgacctgg tcgacgagag aggagcccgg gatctcttca gtgacgatca
    12241 cgtcgtgatc ctgcagcgca agcttggcag cagcctccct gctcacaatc cagggggtcc
    12301 cgtccacccg tgccttcacg tacgtgacac ccgggttcac ccagagattc gtgacaccgt
    12361 aaatggtctc gggacggagt gtcgcagtgg ggatgagcga gtccttccac tcaaacatca
    12421 ccgtcgtgaa cttcaggatc tcggccctgt cgccctcgag aaggtcatgg tcgcccacag
    12481 ggttgtcaca ctgggggcag aaccgcacgg gatgtgcacc cttgttcaca tgtccccctt
    12541 catgcaggtg cagccattgc cactcgataa acttgctata ctgcggatcg acagtggtga
    12601 aacggcgcct ccagtcgatc gagagcccgc atgcggtcat caccctgcga tactcttcgg
    12661 agaagtggcg gactatctcc agcgggtcgg taaatttttc cagggtctcc tgcgggacct
    12721 tgtacaggtc gcggtagagg aaaatggcct tctcgtcgcc cctcgcgatc ctctttgata
    12781 tcccgatgac cggggcgccg gtgacatgga aggccatcgg atagagcacc tgcctgcccc
    12841 tcatcctcca aaaccgtgcg atcacatcag ggacgatgta tgtcctccca tgccccacgt
    12901 gcatggcgcc gctggggtag gggtatgcaa ctgtcaggta aaacttctct ctggacgcag
    12961 ggtccggttc gaatgcatgc tcccatgcgt gcagggcagc ctcctccagc tgccgcatgt
    13021 caggtaggct caagtcctct cacctttctc ctgtgaaaaa taaaagaaaa tgtctatact
    13081 ggccttaatt taatgctgtc gccctcgttg taccgcttga t
//
LOCUS       DAOQYR010000064        12651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_5462_length_12651_cov_9.331137, whole genome shotgun sequence.
ACCESSION   DAOQYR010000064 DAOQYR010000000
VERSION     DAOQYR010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 12651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12651
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5462_length_12651_cov_9.331137"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1302)
                     /locus_tag="PKM87_08210"
     CDS             complement(97..1302)
                     /locus_tag="PKM87_08210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNQ30086.1"
                     /translation="MPPGTRDFQTPAEKADSLLIEIPLESPLSGNEQEIVVEIGRTSG
                     KNGFFFEVDSDTLRFYVRSLDPGAFRSTVLDLLRGHGEQGSAPARLHQVFGDLFDRID
                     SVKSYGIRGKKRIYATYNRERKVAGREHKVKARGSWYYALDHGFEREENALPDEFRNA
                     VIVGDSEEVLRALPSNCIDLVFTSPPYNFGLGYDTGEDGVDWERYFKKLFSVFDECIR
                     VLKYGGRIIVNIQPLFSDYIPSHHVISHYFMKEKLIWKGEILWEKHNYNCKYTAWGSW
                     KSPGNPYLKYTWEFLEVFAKGDLKKEGSREKADITADEFKKWVVAKWSIAPERQMARF
                     DHPAMFPEELVMRALKLFSFEGDVVLDPFNGAGTTCVAAKKLNRNYLGIDISQKYCET
                     AERRLKEIL"
     gene            1639..2445
                     /locus_tag="PKM87_08215"
     CDS             1639..2445
                     /locus_tag="PKM87_08215"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ30087.1"
                     /translation="MQCITFDRYIEAFNVLEKYSVEYPFMLDHLLGVADECFPSGLSM
                     LDIGAGTGKFARSFLERCRVPVHGYTAIEPSADHVAQIRENLDEIPVEKEIILGSFTP
                     ATGFGGRFDLVLLSHCTYCFMPDPEPYLLHALDLVDGRGVAVIYHGSPTNFCSILNLV
                     FGDILPRERVTDPTFTSWNVRDILEKHGIPHEVTCLPGALEVKEIFMPGNEHLLDELI
                     TFSLMVESGSLPQKVLGRAREFLRAIACPGMQGPVLNLGVDAITVRKSPY"
     gene            complement(2460..3152)
                     /locus_tag="PKM87_08220"
     CDS             complement(2460..3152)
                     /locus_tag="PKM87_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30088.1"
                     /translation="MSEDRANPGGAALHPQGESLSPSCAMRSPADSSLSALADHEPFM
                     KAAGSGGLPCRDQCYEIMVDGSGDGHSIALFPGEEVFFAYERGASNNDAEFNAVILAL
                     ENLPPRAHARIRTDSQVVVWHLSATNPRRRANYIQKKAQIEEIIAARHLKVEIQWIPR
                     RENRADRFLKHYIESLCGARGREPLHRRVRRLEVENLRLKTKLAKAMNLLEKRPVVVR
                     DTGGYCMFHEQQ"
     gene            complement(3320..4192)
                     /gene="hisG"
                     /locus_tag="PKM87_08225"
     CDS             complement(3320..4192)
                     /gene="hisG"
                     /locus_tag="PKM87_08225"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNQ30089.1"
                     /translation="MITIALPKGSLEEQTLLLFREADLEVKRSDRDYNPMIADSRIAK
                     VKILRPQEIPRFVEMGYFDLGISGLDWVKESGARVREVARLNYSKTGEGTVKIVVAVH
                     RDEPINDAKEIRPNSRVTTEYPELTREYFGRLGIPVQLFHSFGASEAKVPDLMDVVVD
                     LTETGTTLRRNGLKIIGQIMESYTMIIANRESFEDPAKRKAIEEIVTLLSGVMEARRQ
                     VLLSMNVPTSAMETVISALPAMKKPTVSRLHGIDFYSVQTVVHKQDVNTLIPSLKAAG
                     AEDILEIPITKIVR"
     gene            complement(4591..5868)
                     /locus_tag="PKM87_08230"
     CDS             complement(4591..5868)
                     /locus_tag="PKM87_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNQ30090.1"
                     /translation="MIEGEMVDIFPFPAVRPPAGAAPMVAAVPYDVVTTDEAAACIRK
                     NQSSFLRVSRPDADLPGVSPHDSMVYARAREVFAEFRARGLLKKDHAPAMYLYRVTGN
                     GNDFLGLGCTVSVRDYEENTIRRHELTRYDKEEDRTRHIDAVNAHSGPVVLLFEDRND
                     THGFLDALWNRLGKEDAVVSWDDGTVHRVKCITGEGDLSTLERTFRAIPRLYIADGHH
                     RAKSAVNVCLKRKVEGRLTPEATRFLGVIFAHDRVKIHGYSRIVTDLSGLSAPEFMNL
                     LKGVFSVTPYGEVDTRAFHIPPRHSSQGECHVMHMYLGGAWYECSRPRDGSMDRIEAL
                     DVSVLQREVLTGILGITDPRGDPRLHYLGGARPIGDLENMVKNDGYAVAFSMQPVKVG
                     TVIEIADAGGIMPPKSTWFEPKLLSGLVVHTLD"
     gene            5929..6387
                     /gene="rimI"
                     /locus_tag="PKM87_08235"
     CDS             5929..6387
                     /gene="rimI"
                     /locus_tag="PKM87_08235"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNQ30091.1"
                     /translation="MPAEIALRRARPSDIDRIALIEQESFLDPWKAAVFAEALSYFPE
                     SFFVATVSGEVVGFVLGGLEDTGEEVYGHICNLAVTPAWRKRGIARRLVGRVEQQFAV
                     DLATGVQLEVRVSNRPAQQFYRRLGYRPVLRFAGYYANGEDAIVMMKWFR"
     gene            complement(6397..7602)
                     /gene="hmgA"
                     /locus_tag="PKM87_08240"
     CDS             complement(6397..7602)
                     /gene="hmgA"
                     /locus_tag="PKM87_08240"
                     /EC_number="1.1.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase (NADPH)"
                     /protein_id="HNQ30092.1"
                     /translation="MDEYLKRLREGSLKLYALEKELPPEEAVRVRRRYIEEEAGVPLE
                     RVGSFTIDIERVARKNCENMIGAVQVPVGVAGPLRVNGEYAAGEFYLPLATTEGALVA
                     SVNRGCGAIARAGGADVRILRDGMTRAPVFAARNVAHAREVADWVAAHGDELARAAES
                     TTSHGKFLGAVTTVVGTSVFVRMEFDTKDAMGMNMVTIGSEKAAELISDATGAHLVAL
                     SGNLCTDKKPAAVNVVRGRGRSVCAGVWLDDALISSVFKTDARTMAEVNRRKNLVGSA
                     AAGSLGFNAHAANVVAAMFIACGQDPAHVTEGSMAITTVDEGEGGLYVAVTLPDLPVG
                     TVGGGTGVGTQAECLSLLGVAGGGDPPGSHARKFAEILAAGVLAGELSLLGALAAQHL
                     ARAHKSLGR"
     gene            7702..8610
                     /locus_tag="PKM87_08245"
     CDS             7702..8610
                     /locus_tag="PKM87_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HNQ30093.1"
                     /translation="MRSQWCVRVGKRGAELRRQELLGQGLLDPALKPRREGDWVLFPV
                     TAPVPGTEHCEFETHRGQGDLPRHELIGGIAVMQERDAEDAALLLASRPSIHTVLSPE
                     SDVEGPWRTRRFEVLAGEPTTKTRYIEHGLRFEIDLSEAYFSARLSTERQRLLSMAGP
                     GEIMLDMFAGVGPCALTLARKAAFVAASDLNPGAVLLMERNIAHNRVTNVLPLLCNAS
                     RLPRLLPWKFDRVVMNLPLGAVRFLPAAMELCRPGGTIHCYTLQEKEGEYLEEIETHP
                     VASVRERVVRSYSPGRWHAVYDITLK"
     gene            complement(8625..8834)
                     /locus_tag="PKM87_08250"
     CDS             complement(8625..8834)
                     /locus_tag="PKM87_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNQ30094.1"
                     /translation="MEISARNVFKGKVKSIKYGQILAELVIELPGGLEITSLITRTSA
                     DRMKLVVGKSISTIIKASNVMVATD"
     gene            complement(9170..9565)
                     /locus_tag="PKM87_08255"
     CDS             complement(9170..9565)
                     /locus_tag="PKM87_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HNQ30095.1"
                     /translation="MSSVARFWRKIPQRYNLIGTRCSTCGRYFFPPRSFCPDCRRAGN
                     IVRYKFPGTGSVVTYTIVRTASEQFEQMTPYALAIVQLDEGPRFTAQLVCSPEEVKIG
                     MRVRPVFRRISADGESGVIHYGTKYVPEG"
     gene            complement(9567..10730)
                     /locus_tag="PKM87_08260"
     CDS             complement(9567..10730)
                     /locus_tag="PKM87_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HNQ30096.1"
                     /translation="MRDVAVIGVGCTTFGEKWNTSFRDLFVEAGALALEDAGVSGEQI
                     GAMYVGNMSAGRFIEQEHIGALIADYAGLASTHIPSTRVEAACASGGLSFRQAFISVA
                     SGIEDIVVAAGVEKMTDVASGASVEALAGAADREWEGFVGATFPGLYAMIAHEYMSRY
                     PLTREQLAQVAVKNHYNGARNPIAQFQQEITLDTVLKSTLVADPLRLFDCSPITDGAA
                     AVVLAPLDMARKFCDTPVKVLASAQASDTIALHDRREISTLDATVAAGKRAFTMAGLG
                     PKDIDLVEVHDCFTIAEICAIEDLGFCAKGDAGRLTMEGVTALNGDIPVNTSGGLKAC
                     GHPVGATGIKQVCEVVMQLRGQAGKRQVDGARIGMTHNVGGTGATVAVHILGV"
     gene            complement(10727..11779)
                     /locus_tag="PKM87_08265"
     CDS             complement(10727..11779)
                     /locus_tag="PKM87_08265"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HNQ30097.1"
                     /translation="MVGIITYGVYIPKFRIKVEEIARVWGANPDDISGGLGVYSKSVP
                     DLDEDTATIAVEAARNAMRRRAIDPQEIGAVYVGSESHPYAVKPTACTVGEAIGATPT
                     MTAADYEFACKAGTAGIQTCMGLVASGMVKYGLAIGSDVAQGAPSDALEYTAAAGGAA
                     FLIGNRDPIAEIHRTCSFTTDTPDFWRREGQDYPRHGGRFTGDPGYFRHIEGAVRLLF
                     EQTAKGPKDYTYAVFHQPNAKFPQRVAKILGFTPEQIRPGLVVPRLGNTYSGSSMIGL
                     AATLDCAKAGDRIFVASFGSGAGSDAFDIEVTDAIESGEFRRDEAPGVEKLLENPVFV
                     TYAQYARHKGKIVMQK"
     gene            complement(11773..12483)
                     /locus_tag="PKM87_08270"
     CDS             complement(11773..12483)
                     /locus_tag="PKM87_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ30098.1"
                     /translation="MKSDLRNRLAEKMAGEITLSDSPGKALKKWRMSFNIPQAVLSEH
                     LDVSPSVISDYESGRRKSPGTAVVGKIVDTILSIDEASGGKYIKKFAKILYSDVDDDV
                     IYDIHDYPSPVPLARFAELIQCQTVCGSLSQSIYGYTVVNSLNAITQLSSNEFNRIYG
                     WSTERALIFTDVTSGKSPMVAIRVTPFKPRVVVLQGIEPALVHPLVPKMAEKDRITVL
                     CTEMDVDRVVHILRDEQW"
BASE COUNT         2334 a         3981 c         3765 g         2571 t
ORIGIN      
        1 accggttcat cttctgtttc ggggagatgg aggggtgcaa cgcggtgccg atggtaagaa
       61 agggggtgtg aattcaggat tccctcacgc gaggattcac aggatctcct tcagccgcct
      121 ctccgcggtt tcgcagtact tctgcgagat gtcgatccca aggtaattcc tgttcagctt
      181 ctttgctgca acgcaggtgg tgccggcgcc gttgaagggg tcgaggacca cgtcgccctc
      241 gaagctgaag agtttcaggg cacgcattac cagttcttcc ggaaacatcg cggggtggtc
      301 gaaccgtgcc atctgcctct cgggcgcgat cgaccacttc gccaccaccc acttcttgaa
      361 ctcgtcagcc gtgatatcgg ccttctcccg cgacccttcc ttcttgaggt ctcccttggc
      421 aaatacctcc aggaactccc aggtgtactt gaggtaagga ttgcccgggc ttttccagct
      481 gccccaggca gtgtacttgc agttgtagtt gtgcttctcc cagaggatct ctcctttcca
      541 gatgagcttc tctttcatga agtagtggga gattacatgg tggctcggga tatagtccga
      601 gaagagcggc tggatgttca cgatgatccg cccgccatac ttcaggaccc ggatgcactc
      661 atcgaagaca gagaagagct tcttaaaata ccgttcccag tcaaccccgt cctcgcccgt
      721 gtcgtacccg agcccgaagt tgtagggcgg cgaggtgaac acgaggtcga tgcagttcga
      781 ggggagcgcc cgcagcacct cttcgctgtc cccaacgatg actgcgttcc tgaactcatc
      841 cggcagcgca ttctcctctc tttcgaaccc gtggtcgagc gcatagtacc acgaccctcg
      901 tgccttcacc ttgtgctccc ggccagccac cttccgttcc cggttgtaag tggcatagat
      961 gcgcttcttc ccccggatcc cgtagctttt caccgagtcg atccggtcga acaggtcccc
     1021 gaacacctgg tgcaggcggg cgggagcgct cccctgctct ccatggccgc ggaggagatc
     1081 gaggacagtg ctcctgaatg ccccggggtc gagggatcgg acgtagaaac ggagggtatc
     1141 actgtcaact tcgaagaaga acccgttctt ccccgaagtg cggccgattt ccacgacgat
     1201 ttcctgttca ttcccggaga ggggggattc gagtgggatc tctatcagga gagagtccgc
     1261 cttctcagcg ggggtctgga agtcccgcgt tcccggcggc atatcaggtg tcaccacctc
     1321 tcactgcctg gccaggtaag agccgccctg catccatacc gaccaacctc catcgcgggt
     1381 cccgctggtg ttgtcgactg cgtcgcatcg cgactgtccg gtggaaggtc cgggatggca
     1441 atccctcgaa ttccatcaag aaagagtgga cctgcagaag agaaatacct gcaggttgcg
     1501 tgaggagcga aaagacccac cccctctttt cccccagggt gttcaggggc tcttcgaatc
     1561 tggaccagag gttcccggca cgtttccggg agtcctcagg aacattattt ccgaacgaat
     1621 gaaagagact aaagaaccat gcagtgcatc acgttcgacc gctacatcga ggcattcaat
     1681 gtgctcgaga agtactcggt cgagtacccc ttcatgctcg accacctcct cggcgtcgct
     1741 gatgagtgtt tccccagcgg tctctccatg ctcgatatcg gcgccgggac gggaaaattc
     1801 gcgaggtctt ttctcgaacg atgcagggtt cctgtccatg gctacaccgc gatcgaaccg
     1861 tcagcagacc atgtcgcgca gatccgggag aacctggacg aaatcccggt agaaaaagag
     1921 atcatcctcg gttctttcac ccctgcgacc ggctttgggg ggaggttcga cctcgtcctg
     1981 ctctcccact gcacttactg tttcatgcct gaccccgaac cgtacctgct gcatgcgctt
     2041 gacctggtgg acggacgcgg tgtagcggtc atctaccacg gcagcccgac gaacttctgc
     2101 tcaatcctga acctggtttt cggggacatc ctccccaggg agcgggtcac tgaccccacc
     2161 ttcaccagct ggaacgtgcg ggacatcctg gagaaacacg gcatccccca tgaggtcacc
     2221 tgccttccag gtgcattgga agtaaaagag atcttcatgc caggcaacga acacctcctc
     2281 gacgagctga tcacgttctc tctcatggtg gaatcaggct cccttcccca aaaggtcctc
     2341 gggagggcca gagaattttt aagggcgatt gcctgccccg gcatgcaagg cccggtgctg
     2401 aacctcgggg tagacgcgat cacggtgagg aagagtccgt actgacgccc caccatcttc
     2461 tactgctgtt catggaacat gcagtatccg cccgtatccc ggaccactac cggccgtttt
     2521 tcgagcaggt tcatggcttt tgcaagtttt gtcttcagcc ggaggttctc cacttcgagg
     2581 cgcctgaccc gcctgtggag gggttcgcgc ccccttgccc cgcagaggct ctcgatgtag
     2641 tgcttcagga aacggtctgc ccggttctct ctccgcggga tccactggat ctctaccttc
     2701 aggtgccggg cagcgatgat ctcctcgatc tgcgccttct tctggatata gttcgcacgc
     2761 cgccggggat ttgtggcaga gaggtgccag acgaccacct ggctgtcggt acggatccgg
     2821 gcatgggccc tgggagggag gttctcgagt gccaggatga cggcgttgaa ctcggcgtca
     2881 ttgttggatg ccccccgctc gtacgcaaag aagacctcct cgccaggaaa gagggcaata
     2941 ctgtgcccgt cgccgctccc gtccaccatg atctcgtagc actggtcccg acagggcagg
     3001 cccccggatc cggcggcttt catgaagggt tcatggtcgg ccagagcgga taaagaagaa
     3061 tcagcaggag aacgcatcgc gcaggagggg gagagggact caccctgggg gtgaagggca
     3121 gcaccgccgg gattcgcgcg atcctcgctc attcccacct gaaggagtgg ttgcactctc
     3181 accatattta attattttgc tcgttttttg tgttttaggt caggattcgc gaacggtttt
     3241 ccgatccaac aggttaaaca caagaaaaat ttattctgcg aaatactgag aatatgggtt
     3301 attttataat atgggatgtt tagcggacga tttttgtaat agggatctcg agtatgtcct
     3361 ctgccccggc agccttcagg ctggggatga gagtattcac gtcctgcttg tggaccacgg
     3421 tctgcacgct gtagaagtcg atcccgtgca gccggctcac ggtgggcttc ttcatggccg
     3481 ggagggcgga aatcaccgtc tccatcgccg aggtcggcac gttcatcgag agcagcacct
     3541 gccgcctcgc ctccatcacc ccggagagga gggtcacgat ctcctctatc gcctttcgct
     3601 tcgccgggtc ctcgaaggac tcgcggttcg cgatgatcat cgtgtaggac tccatgatct
     3661 gcccgatgat cttcaggccg ttcctccgca gcgtcgtccc ggtctcggtc aggtccacga
     3721 ccacgtccat caggtcgggc accttggcct ccgacgcacc gaatgagtgg aagagctgga
     3781 cggggatacc gagccggccg aagtactcgc gggtcagctc ggggtactct gtcgtgacac
     3841 ggctgttcgg acggatctcc tttgcgtcgt tgatcggctc gtcccggtgc actgccacga
     3901 cgatcttgac cgtcccctcg ccggtcttgc tgtaattgag ccgggccacc tcgcggaccc
     3961 tggcaccgct ctccttcacc cagtcaagcc ccgatatccc gaggtcgaag tagcccatct
     4021 ccacgaaccg ggggatctcc tgtgggcgga ggatcttcac cttggcgatg cggctgtctg
     4081 cgatcattgg gttgtagtca cggtcggacc gcttcacctc gagatccgcc tcgcggaaga
     4141 gaaggagggt ctgctcctcc aggctgccct tgggaagtgc gatagtgatc atagtatacc
     4201 atataaggcc gttcaggaaa aaaatccgcc tctgtgcaat accaacgaag agaaatcctt
     4261 ctcgttaatg caggcccttc catgaacctg ttgatatgga gtggtgccgc gcagggactg
     4321 aaattggccg ggatccccgg gagcaccgag atagagagat gagccacaga aagatgatac
     4381 gcaaggtgtg ccggaccagt cctgatttgg agtataacgc atcagggatc ggaggagatt
     4441 cccgcgacag gggcggccat tgttctcata tgacccggga aacgggcagg cagaatggat
     4501 tttcatcctt cctgtcaagg ggtgactttt tcttaaaaaa agaccgtgaa aggcagtttt
     4561 ggaacaaacg tcgccgaagc gagaagatac tcagtccagg gtatggacca cgaggccaga
     4621 gaggagcttc ggttcgaacc aggtggactt cgggggcatg atgccccccg catccgcgat
     4681 ctcgatcaca gtcccgacct tcaccggctg catcgagaag gccaccgcgt acccgtcgtt
     4741 cttcaccatg ttctcgaggt ctccgatcgg ccgggcaccc ccgagataat ggaggcgggg
     4801 gtcccccctc gggtccgtga ttccaaggat cccggtcagc acctcccgct ggaggacaga
     4861 gacatccagt gcctcgatcc ggtccatgct cccgtcgcgc gggcggctgc actcgtacca
     4921 cgcaccgccg aggtacatgt gcatcacgtg gcactccccc tgagaagaat gccgcggggg
     4981 gatgtggaat gcccttgtgt cgacctcgcc gtagggggtg accgagaaga cccctttcag
     5041 gaggttcatg aactccgggg ccgagagtcc cgagaggtcg gtgacgatcc ggctgtaccc
     5101 gtggatcttc acgcggtcgt gggcaaagat caccccgagg aaccgggtag cctcgggggt
     5161 gagccgccct tccactttcc gcttgaggca gacgttcacc gcggacttgg cccggtggtg
     5221 cccgtcggcg atgtagaggc gggggatggc ccggaaggtc ctctcaaggg tggagaggtc
     5281 cccctccccg gtgatgcact tcacccgatg gaccgtgccg tcatcccaac tgaccacggc
     5341 atcctccttc ccgagcctgt tccagagcgc atcgaggaac ccgtgggtat cgttcctgtc
     5401 ttcgaagagg agcaccacgg gcccgctgtg agcgttcacc gcgtcgatgt gccgtgtgcg
     5461 gtcctcctcc ttgtcgtacc gggtcagctc gtgccgccgg atggtgttct cctcgtagtc
     5521 gcgcacggag accgtgcacc cgagcccgag gaagtcgttc ccgttcccgg tcacgcggta
     5581 gaggtacatg gcaggggcgt ggtctttctt gaggaggccc cgtgcacgga actcggcgaa
     5641 cacctcgcgt gccctggcat acaccatgga atcatgggga gagaccccgg gaaggtcggc
     5701 gtcgggcctg ctcacccgaa ggaagctcga ctggttcttc ctgatgcagg cggcagcctc
     5761 gtccgtggtc accacgtcgt aggggaccgc ggcgaccata ggggctgcac cggccggggg
     5821 gcggaccgcg gggaaaggaa aaatatccac catttctccc tctatcattc tccctggcag
     5881 tatattatag caatgaagac catagtgtaa aagacggtag acctctccat gcccgccgag
     5941 atcgccctac gccgggccag gccaagtgac atcgaccgca ttgccctgat cgagcaggag
     6001 tcgttcctcg acccatggaa ggcagcggtc tttgccgagg cgctctccta cttccccgaa
     6061 tccttctttg tcgccactgt ctcaggcgag gtcgtgggtt ttgtactcgg cggcctcgag
     6121 gatacgggcg aagaggtcta cgggcacatc tgcaacctcg cggtcactcc tgcatggaga
     6181 aagaggggga tcgcaaggcg actcgtcggg cgggtggagc agcagttcgc ggtggatctt
     6241 gcaaccggcg tgcagctcga ggtccgggtc tcgaaccgcc ctgcgcagca gttctaccgg
     6301 aggctcggct atcgcccggt cctccggttc gctggctact acgcgaacgg ggaggacgcg
     6361 atcgtcatga tgaagtggtt ccgctaaaga tccccttcag cggcccagcg acttgtgagc
     6421 ccgtgccagg tgctgggcgg cgagtgcgcc caggagcgag agttccccgg caaggacgcc
     6481 tgcggcgagt atctcggcga acttccgtgc gtgtgacccg ggagggtccc cgccgccggc
     6541 caccccgagc agagagagac actcggcctg ggtccccacc ccggtcccgc cgccgactgt
     6601 gccgacggga aggtcgggga gggtgactgc aacatacagt cctccctccc cctcgtcgac
     6661 cgtggtaatt gccatgctcc cctcggtgac gtgcgccggg tcctgcccgc aggcgatgaa
     6721 catagccgcc acgacgtttg cggcgtgggc attgaagccg agcgagccgg ccgccgcgga
     6781 cccgacgagg ttcttcctcc ggttcacctc ggccattgtg cgtgcgtcgg tcttgaagac
     6841 agaggatatg agggcgtcgt cgagccagac accggcgcag acgctcctcc cccgcccccg
     6901 gaccacgttc actgcggctg gcttcttgtc ggtgcagagg tttcccgaga gcgcgacgag
     6961 gtgcgccccg gtcgcgtcgc tgatcagttc cgcggccttc tcgctcccga tggtgaccat
     7021 gttcatcccc atggcgtcct ttgtgtcgaa ctccatcctg acaaagaccg aggtcccgac
     7081 aacagtcgtc accgcgccga ggaacttccc gtgcgaggtg gtggactccg ctgcccgcgc
     7141 cagctcgtcc ccgtgggcgg cgacccagtc cgcaacctca cgggcatggg cgacgttccg
     7201 ggcggcaaag accggcgccc gtgtcatgcc atctcggagg atcctgacat ctgccccgcc
     7261 agcccgggcg atcgccccgc acccccggtt caccgaggcc acaagcgcac cctccgtcgt
     7321 cgcaagcggg aggtagaact cgcccgccgc gtactcgcca ttgacccgga gcggccctgc
     7381 gacccccacc ggcacctgga ccgcaccgat catattctcg cagttcttcc tcgccacccg
     7441 ctcgatatcg atggtaaagg acccgaccct ttcgagcggg acgcccgcct cctcctcgat
     7501 atagcgccgc ctcacccgga cagcctcttc cggggggagt tccttctcca gggcatagag
     7561 cttgagcgac ccctccctca gccgcttcag gtactcgtcc atactccatc atgggagggg
     7621 cgggggaata acccttcttt cgagggggat acactcatcc tcctggcctt cctctggtac
     7681 atggaaatgc aggaagggtc cgtgagatct cagtggtgtg tcagggtcgg gaagcggggc
     7741 gcagagttgc gcaggcagga gctcctcggg caggggctcc tcgacccggc cctcaaaccc
     7801 cggagggagg gcgactgggt cctcttcccg gtcacagccc ctgttccggg taccgagcac
     7861 tgtgagttcg aaacccacag ggggcaggga gacctcccga ggcacgagct gataggcggg
     7921 atcgcggtca tgcaggaacg ggacgcggag gatgccgcgc tcctccttgc ctcccgcccc
     7981 tccatccata cggtgctctc ccccgaaagc gacgtcgaag ggccctggcg cacgagacgg
     8041 ttcgaggtgc ttgccggtga gccgacgaca aagacccgat atatcgaaca tggcctgcgc
     8101 ttcgagatcg acctgtcgga ggcgtacttc tcggcacgcc tctcaaccga acgccagcgc
     8161 ctcctctcca tggcgggccc tggcgagatt atgctcgaca tgttcgcggg ggtgggcccc
     8221 tgcgctctta cgctcgcccg gaaggccgcg ttcgttgccg catcagacct caaccccggg
     8281 gcagtgctgc tgatggagag aaacatcgcc cacaacaggg taacaaacgt ccttccactt
     8341 ctctgcaatg catcccgcct cccgcgcctc ctcccctgga aattcgacag ggtggtgatg
     8401 aacctcccgc tcggggccgt caggttcctt cccgccgcca tggaactctg caggccgggc
     8461 ggaacgatcc actgctacac cctgcaggag aaagaagggg agtatctcga agagatcgag
     8521 acgcaccccg tcgcatctgt ccgggaacgg gtggtccgct cctactcccc gggccggtgg
     8581 cacgcggtat acgacatcac gctgaaatga gcctcgtcaa atccctaatc ggttgcaacc
     8641 atcacgtttg aggctttgat aatcgttgaa atgctcttcc cgacgacgag tttcatccgg
     8701 tccgccgacg tcctggtaat cagcgaggtg atctccagtc cccccggcag ttctatgacc
     8761 agctccgcaa ggatctggcc gtatttgatc gactttacct ttcccttgaa cacgtttcgt
     8821 gcgcttattt ccatggtcgt caccgtgtgc caattgtaaa gtacttttta tatattttgg
     8881 catggtgaca gacatacggg gatgtcctgg tatgtcatct ggtaaagagt gccgggaatg
     8941 gaagaaccat gtcataaccg tgaagaaacg gtatgaaaga atgaacgatc aggcaaacag
     9001 ggtgtgatcc gttcgcactc cctggattaa gaggggaagt tccccgtgaa tgagccagac
     9061 cgatcgccat gccggagttt tcactcaacc ggttcgccat ggggatcgtt ctctctcagg
     9121 ggtgatatgc tgagaagagg ggcaaccagt acgaagagaa aagggatttt tagccttccg
     9181 ggacatactt ggtcccgtag tgaatgacgc ccgattcccc gtcggccgag atgcggcgga
     9241 agacgggcct gacacgcatc ccgatcttca cctcttccgg gctgcagaca agctgtgcgg
     9301 tgaacctcgg cccctcgtcg agctgtacaa ttgcaagagc gtatggcgtc atctgctcaa
     9361 actgctcgct tgcggtgcgg acgatcgtgt aggtcaccac gctgccggta ccggggaact
     9421 tgtatctgac aatatttccg gcacggcggc agtcagggca gaaggaccgg ggcgggaaga
     9481 agtatctccc gcaagtgctg caccgggtgc cgatcaggtt gtaccgctgg gggatcttcc
     9541 tccagaaccg cgcgacagag gacatctcac acccccagga tatgcacggc gacggtcgcg
     9601 cccgtgcccc cgacgttgtg cgtcatcccg atcctcgccc cgtcgacctg ccgcttcccg
     9661 gcctggccac ggagctgcat gaccacctcg cagacctgct tgatcccggt cgcaccgacg
     9721 gggtgcccgc aggccttcag cccgccgctt gtattgacag ggatgtcccc gttgagtgcc
     9781 gtgaccccct ccatggtcag cctgccggca tctcccttgg cgcagaaccc gaggtcctcg
     9841 atcgcgcaga tctcggcgat ggtgaagcaa tcgtgcacct ctacgagatc gatatccttt
     9901 ggcccgagcc cggccatggt aaaggcgcgc tttccggctg cgacggtcgc gtcgagggtg
     9961 ctgatctccc tcctgtcgtg gagggcgatg gtgtcgctcg cctgggcgct cgcgagcacc
    10021 ttcaccgggg tatcgcagaa cttcctggcc atgtccagcg gcgcgaggac gaccgccgcc
    10081 gcaccgtcag taatcggaga acagtcgaag agccgcagcg gatccgcgac gagcgtcgat
    10141 ttcaggacag tgtccagggt gatctcctgc tggaactgtg cgatggggtt tctcgcgcca
    10201 ttgtagtggt tcttcactgc gacctgcgcg agctgctccc tggtgagggg gtagcggctc
    10261 atgtactcgt gtgcgatcat ggcatagagc ccggggaacg tcgcgcctac gaacccctcc
    10321 cactcacggt ctgcggcgcc ggcgagcgct tccacgctcg caccggaggc gacgtccgtc
    10381 atcttctcta cacccgctgc aaccacgatg tcctcgatcc cggacgccac cgatatgaac
    10441 gcctggcgga acgagagccc gccggaggcg cacgcggcct cgaccctggt cgaggggatg
    10501 tgggtggagg caaggcctgc gtagtccgcg atcagggcgc cgatatgctc ctgctcgatg
    10561 aaccggcccg cgctcatgtt gccgacgtac atggccccta tctgctcgcc ggagaccccc
    10621 gcgtcctcga gcgcgagcgc acctgcttcc acgaagaggt cccggaacga cgtgttccac
    10681 ttctccccga aggtggtgca tcccacccct atcactgcaa cgtctctcat ttctgcatca
    10741 cgatcttgcc cttgtgtctc gcatactgtg cataggtcac gaagaccggg ttctcaagga
    10801 gcttctcgac ccccggggcc tcatcgcggc ggaactcgcc ggactcgatc gcatcggtga
    10861 cctcgatatc gaaggcatcg ctccccgcac ctgacccaaa cgacgcaaca aagatacggt
    10921 cgccggcctt cgcgcagtcg agcgtcgccg caagcccgat catggacgat ccgctgtagg
    10981 tgttcccgag gcgcggcacc accaggccgg gcctgatctg ctctggggtg aacccgagga
    11041 tctttgcgac ccgctgggga aacttcgcgt tcggctggtg gaagaccgcg taggtgtagt
    11101 ctttcggccc cttcgcggtc tgctcgaaga gcagacggac cgcaccctcg atatggcgga
    11161 agtagccggg gtcgccggtg aacctgccgc cgtggcgcgg atagtcctgc ccctccctcc
    11221 tccagaagtc gggggtgtct gtggtgaacg agcaggtgcg atggatctcc gcgatgggat
    11281 cccggttgcc gatcaggaac gcggctcccc cggcggctgc cgtgtactcg agggcgtcgc
    11341 tcggtgcgcc ctgcgcgaca tcagacccga tggcaaggcc gtacttcacc atgccgcttg
    11401 ccacgaggcc catgcaggtc tggatgcccg cggtccccgc cttgcaggcg aactcgtagt
    11461 ccgccgcagt catcgtgggg gtggccccga ttgcctcccc aaccgtgcag gcggtgggct
    11521 tgaccgcgta ggggtgggac tcgcttccca cgtagaccgc gccgatctcc tgagggtcga
    11581 tcgccctcct ccgcattgca ttccttgctg cttccaccgc gatggtcgcg gtgtcctcgt
    11641 cgagatcggg gaccgatttc gaatagactc caagcccccc gctgatatcg tccgggttcg
    11701 ccccccagac cctggcgatc tcttctacct tgatgcgaaa ttttggtata tacacaccgt
    11761 atgtgatgat gcctaccatt gctcgtccct cagtatgtgc acgaccctgt ccacgtccat
    11821 ctcggtgcag agcaccgtga tcctgtcctt ctccgccatc ttcggcacca gcgggtgcac
    11881 gagcgcgggt tcgatcccct gcagcaccac cactcggggc ttgaaggggg tgacccgtat
    11941 cgccaccatt ggggacttgc ccgaagtgac atcggtgaat atcagcgccc tctccgtgct
    12001 ccacccgtag atgcggttga actcgttcga ggagagctgg gtgatggcat tgaggctgtt
    12061 gaccaccgtg tatccataga tggactgcga gagtgacccg cagaccgtct ggcactggat
    12121 gagttccgca aaccgggcca gcgggaccgg cgacgggtaa tcgtggatat cgtagatgac
    12181 gtcgtcatcc acgtctgaat agaggatctt tgcaaatttc ttgatgtact tgcccccgct
    12241 cgcctcgtcg atggagagga ttgtatcgac gatctttccc accacggccg tgccggggct
    12301 ctttcgcctt ccgctctcgt agtcgctgat cacggaaggg gacacgtcca ggtgctcgga
    12361 gagcactgcc tgggggatgt tgaaactcat cctccacttc ttcagtgcct tgcccggcga
    12421 gtctgaaagc gtaatctctc cggccatctt ttcggccagc cgattccgca aatcagattt
    12481 catggctcat tatgcgggtt cccattttat ataattaacg aatggggatt cgacatttca
    12541 cgaaaaaccg gtgcccggtt gcccgggttg gatatccata aatagtaagc atctcaattc
    12601 tgatataatg atccttccag aggagctcca gtgcctcaag gcgatcgcgc t
//
LOCUS       DAOQYR010000065        12571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_5498_length_12571_cov_8.895334, whole genome shotgun sequence.
ACCESSION   DAOQYR010000065 DAOQYR010000000
VERSION     DAOQYR010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 12571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12571
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5498_length_12571_cov_8.895334"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..1774)
                     /locus_tag="PKM87_08275"
     CDS             complement(203..1774)
                     /locus_tag="PKM87_08275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ30099.1"
                     /translation="MHSDPSDTAMKIQELPADLESANQALNKENARLKGMIDDCGRIK
                     NELKMSEENLKGFLASLPHSAHLIDREGTVLISSEVSPARLGISLDEYIGTNIFAHLP
                     PGLAVERREHFERILRSRETVSWEDSREGRHYAHYGSPVLDNEGNVDRIAHIALDITD
                     RKKIEDAIRESEEKYRAVVEMSPDAIVIHQDGRIVYANPASAQLFHLPASDVLIGTDA
                     FARIHPDFLAIARENTRIDLEGRDTPPTEIQVTLRDGTLATFEGRGRKLIFQGRPAIQ
                     VVLRDISERKKAEEQLQEFSRNLARANEELELFISIASHDLQEPIRNIVTSTQRMLRD
                     SRESDAGSFEKHLKTIETAALRMHALINSLREYSAVRKRDLQSVSMDNALSSALENLR
                     QLIKETGATVTHDPLPNALADPTRIIQVFQNLIENAIKFRKEGVPLVIHISASRDGDV
                     WEFSVRDNGIGIPSDYYDRIFVLFEQLHQRDVYPGTGLGLALCKKIIERNRGRIWVSS
                     VIGEGSTFFFTLKAE"
     gene            complement(1995..2510)
                     /locus_tag="PKM87_08280"
     CDS             complement(1995..2510)
                     /locus_tag="PKM87_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30100.1"
                     /translation="MNLYLQSFLLFAVLAAWLSGAGCLTGSLQENLTQSVSPVQAGHA
                     NTTALPAEYPDITGTWKADDGEGYYLNTTIQRVGIGVNTWVFTSQHGHIVEGYKIFSQ
                     PDGSMAHQTLAGMFDPDKKSIYFIDQPGGWAKGTLTDPDTIFITLTYSSGRDTMGNTM
                     ALTMTLHRQGG"
     gene            complement(2818..3663)
                     /locus_tag="PKM87_08285"
     CDS             complement(2818..3663)
                     /locus_tag="PKM87_08285"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNQ30101.1"
                     /translation="MPSDRITIGRFSTLTHLTQKALRYYDSKGILVPAVKDRFTGYRY
                     YTVTQVESALKIRTLCTLGFGLSEIGDILTAHSAQDRDAVRKLVERRKHEAQSEIARL
                     EKVLSFIIENRDFSELFAMNVSEPVIKEIPAMRVLSGRRTGTYEEVCGQVSAALMEII
                     FSPENQKKGVTITGPFLSLCYDGEYRETDADIEMAIPIHGPVVVSDPGITVRTLPAVK
                     AVSVVYRGPYEHEGFSVAFEKAFRYAAENGLEPAGPDRQVYLNNPDNTPAEELLTEVQ
                     IPVAG"
     gene            3769..4488
                     /locus_tag="PKM87_08290"
     CDS             3769..4488
                     /locus_tag="PKM87_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ30102.1"
                     /translation="MHINGVRLLHDRFPTDCSYPFNLEIFRKTDWIGFFRPITFFIGE
                     NGTGKSTLLRAIAKKCNISIWEDERRLKFHFNRYEDEMFRYIDVEWSGKQVAGSFFSS
                     EMFRYFAEILDEWAIADPGTLSYFGNDSLRTRSHGQSHMMFYTSRYQREGLFLLDEPE
                     NALSPRTQIELLHLLRKATSRDNVQFLIATHSPILLAYPGADIYSFDRTPIQKVGYEE
                     TEYYRAYRDFLNHREKYLDWS"
     assembly_gap    4489..4588
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4589..5275)
                     /locus_tag="PKM87_08295"
     CDS             complement(4589..5275)
                     /locus_tag="PKM87_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ30103.1"
                     /translation="MIECAGLQKIYQMGNVGVRALDGVSLTIGRGEFVGIMGASGSGK
                     STLLHMLGLLDVPTSGEITLDGIDVNTLNDYEKTMFRLYKLGYVFQDYALVPELTVAE
                     NVFLPAMLRSDRTDEEYTTATFDILDRIGLSDRRWHLPKELSGGEQQRVAIARAIVNR
                     PDILFADEPCANLDTENSRRVLDLFEEINREMHQTIVMVSHEDWHKKYFDRIIRLRDG
                     KIVEDNHALS"
     gene            complement(5280..6494)
                     /locus_tag="PKM87_08300"
     CDS             complement(5280..6494)
                     /locus_tag="PKM87_08300"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ30104.1"
                     /translation="MGKKELKVALFLAWRSLRRGSRSSAILTILIVGMCFTNMIFLPS
                     LFAGIGQTIKTQIVDYEVGNVLVSPQAGEQYITDLDATLDLINSMPGVERATPHYSKG
                     ATLKYRQRILGSSIRAIRPTDEKSVSPIHTKMIAGSYLGESDTGEIIIGKIIAGDATV
                     SQEDEFYSSLGGVRVGDSITVEFGNGYTKDYRVKGIYSTGWTSSDNSALITWSDMEIV
                     EGESLDKAEYITIKTKPGYSEKFVKDMLMSYGVSPQVHTTDDLLSKAIGSVLQSFDII
                     NMVSLIVSIIITTVVLFIVITIKTINSRKQIGILRAIGVQKEVIMHNYGFQVIILALF
                     GIVLGIAIALLLALYLAYNPIVTPEWSAYMYLTPMDLVSNSLILLASAIVAGYVPAYQ
                     VSREEIQSSMRA"
     gene            complement(6470..7756)
                     /locus_tag="PKM87_08305"
     CDS             complement(6470..7756)
                     /locus_tag="PKM87_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HNQ30105.1"
                     /translation="MQYENNRSINLFIAGIFILLFAGACCTHVFAAEPTVIITDYKVT
                     PSVLAPEGYGTIQVTLKNTASSASLRESSGVSVSGEFQTTKSTDIPAIIDSVSLMGGE
                     IRVIDGSYHHFGAIGPGQSLDVTFSIQAPKKEGLYFPEVWVEINGGKNVRYPVPVNVN
                     TAQYVMQSPTLVIYKELPESINPGESFPVVLIIRNAGEIRASDIVLSTATSTTSLGSK
                     GPNTLTIEPLNGGENRTVTLEFITDRFVAVGLQKVLLAFDYKLPDGTARHQEETIEVP
                     IMGKAELGFVSVDTTPRRVTAGTPFDLTIRIENTGTGEAKQVTAVIDLPMTGNRQSFI
                     GKIKPNNDAPALFMLDGGSGGTYEYHVTVTYTDDLGTHTVVFPMSLQVTPEDYTGTIV
                     LVVIIVLVAGFIAYRYWYLPKKNGNGALPWVKRNSK"
     gene            7935..8516
                     /locus_tag="PKM87_08310"
     CDS             7935..8516
                     /locus_tag="PKM87_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNQ30106.1"
                     /translation="MARLFPEYREEVQKKIIATAHTLFHEKGYSNTKMSEIAAALGVT
                     KPTLYHYFDTKEKLFIAVAEHERKMLQEFIIESFTDRDFVAGATAFFDTIMEGFLKNI
                     GPESVAIITRDDHLRQIILDDREKFIDVIAGFLAERKATGEIREDADVRILACTFNAL
                     FQGLLIYAMQGMRTDDLRLVWNSAVKGLTQKGS"
     gene            complement(8633..10945)
                     /locus_tag="PKM87_08315"
     CDS             complement(8633..10945)
                     /locus_tag="PKM87_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrophobe/amphiphile efflux-3 (HAE3) family
                     transporter"
                     /protein_id="HNQ30107.1"
                     /translation="MKNPFERLAYAVTTHPRIVAGVAVLVFAIALFGVTLVTMETGDD
                     TYIDKTTPRGALLSHYSDAYGSDAIMIIVESDDITQVECLHYIERLQADVANQQYVER
                     VSGIVDLLRQGNGGTLPGSSAEINQIVSQAPPEVVDRYIPSKMMTISVVSLQPGISSD
                     RQEQALNNIRSIVSLSNPPPGVTVILSGSPAFSQEMQQEMGSSMGILILAAMVLMVVA
                     VMTLFNHVRYTLLPVIVVATGLILTFGFMGLAGIPISMVVIGAFPVLIGIGIDYAIQI
                     HSRLDEEVRKSSLPDAITTTITKTGPSVLIAMLATSMGFIAMVFGPIPMVGDFGITCT
                     IGVISCYLAALIIVPVFGILIRYCPREPKGKINDVAACELGWKGCDTTPVHQEGSRGS
                     FIEKYDAFLGAVALKIAKNPVPILMLVFMVAIVGFNLDNDVPISADEKTFVPSEMPAL
                     QNMNKVTRAMGSTSTIPVIMTGEAVLNPDTLTWIRDFGEYEIEHNDKITGVTSIATLL
                     AQYNNGNLPTTEGEVQQVLDRIPAVTKDRYLNGKIETVLEFSTVDMEISQARSLVQLI
                     ERDVAWNSPPPGVTARVTGQIEMFAALMDDISDSKLIMTVLGFAFILAFLLVVYRRVH
                     AVSPLLPIVAIVGWNGAIMYLLGLDYTPLTAVLGSMTIGVASEYTVLIMERVDEELAR
                     GLDLFSAIQTSVQKIGAAITVSGLTTVFGFSALILSEFNIISNFGITTVITVGFSLLG
                     AILIMPAVISLMYRFEYRPANNRKKVSTSE"
     gene            complement(11459..11863)
                     /locus_tag="PKM87_08320"
     CDS             complement(11459..11863)
                     /locus_tag="PKM87_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNQ30108.1"
                     /translation="MAMMNPEMMTAFMSGKIFPLATASKEGEPNVAPMGAVYPRDPET
                     IWIGNQFMKETIKNVLENPRACLYVWGPGITGCFKVKGDIEVKTSGEDYETMKKEVAL
                     AKPGLFCRSLLVMKVTEVYDCLPGEHAGDRLI"
     gene            complement(12014..>12571)
                     /locus_tag="PKM87_08325"
     CDS             complement(12014..>12571)
                     /locus_tag="PKM87_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HNQ30109.1"
                     /translation="GIIHQEMPKPVDGRMGGFQLWVNLPRSHKMIDPRYQEVPGNDVP
                     IISPQRGVRIRIISGTIGEVTGPVQDIVGFPEFFDVMLGPDVPFSHEVSAEHTAAAYV
                     IGGSGHFDLRQEKEMGNRTMILFSREGKRVHVVAGSDGARFLYLSGMPHHDPIAWRGP
                     IVMNTQEELRQAFDEYRKGTFLRKR"
BASE COUNT         2728 a         3428 c         3402 g         2913 t
ORIGIN      
        1 cctccatcgc cctgttccag gcaatcacct ttccctcgcg gtcgatgacc agcgtcgcat
       61 ccggcaaaaa gtcaataacc tcatcctgaa acgcatgccc ggagagttgc attcacctgc
      121 tcctgatcag aggatgtacg gtgattaggt aagaagattc gttcttcctc tcctgcctgt
      181 caggcgaaca gaaaatgaat gcttattccg ctttcagggt aaaaaagaac gtactgcctt
      241 ctccaataac agatgatacc cagattcgcc ccctattacg ttcgatgatc tttttgcaga
      301 gggcaagtcc gagtccagtg cccggatata catccctctg gtggagctgt tcgaagagaa
      361 cgaagattct gtcataatag tcggacggaa tgccgatccc gttatccctg accgaaaatt
      421 cccatacgtc gccatcacgg gaggcagaga tgtgtatgac cagcgggacg ccttcctttc
      481 ggaacttgat ggcgttctcg atgaggttct ggaagacctg gatgatccgg gtcgggtcgg
      541 cgagggcatt cggcagggga tcatgcgtga cggttgcgcc cgtctctttt atgagctgcc
      601 ttaagttttc cagcgcgctg gaaagcgcgt tatccatgga gactgactgc agatccctct
      661 ttctcacggc cgagtactcg cggaggctgt taatcagcgc atgcatgcga agggctgccg
      721 tttcgatggt ttttaagtgt ttttcaaagg aacccgcatc gctttcgcga gaatctcgca
      781 acatccgttg tgtcgaggtc acgatgttcc ggattggttc ctgaaggtca tgtgaggcaa
      841 tcgagatgaa aagctcaagt tcttcgttgg cccgcgccag gtttctcgaa aattcctgca
      901 attgttcttc tgccttcttc cgttccgata tatcgcgcag caccacctgg atcgcgggtc
      961 tgccctggaa gatcagcttc ctccctctcc cttcaaatgt tgccagggtc ccgtcacgga
     1021 gtgttacctg tatctctgtg ggtggggtgt ctctgccttc aaggtcaatc ctggtgttct
     1081 cccgtgcgat cgcaaggaaa tcagggtgga tccgggcgaa tgcatccgtg ccaataagca
     1141 catctgacgc cgggaggtgg aagagctgcg cacttgcagg attcgcatat acgatccggc
     1201 cgtcctggtg gattacgatg gcgtccggtg acatctcgac gaccgcacgg tacttctcct
     1261 cgctttcgcg aatggcgtcc tcgatcttct tccggtcggt gatatccagg gcaatatggg
     1321 cgatgcggtc tacgtttccc tcattgtcaa gcacagggga tccatagtga gcatagtgcc
     1381 ggccctcccg cgaatcctcc catgagactg tttccctgct ccggagaatc ctttcgaaat
     1441 gttctctcct ttctactgca agcccggggg ggagatgggc aaagatgttg gtcccgatat
     1501 actcgtcgag gctgatccca agtcgtgcag gagagacttc actgctgatg agcacggtgc
     1561 cttccctgtc aataagatgg gccgagtggg ggagcgaggc caggaaccct ttcaggttct
     1621 cttcgctcat tttcagctca ttcttgatcc gcccgcagtc atcgatcatc cctttcagtc
     1681 tcgcattctc tttattgagc gcctggtttg ccgattccag gtcagccggg agttcctgga
     1741 tcttcatcgc cgtatcagag ggatcactat gcacaatgtc atcccgcggt ggattcgtat
     1801 tgtgtccatt cccagaacgt ggcattccct ctttcactcc atcagcccct gccggatatc
     1861 atgataatcc aaaaagagaa tgggttcgca tgacctctta ttcattttgg agggggggga
     1921 aatttcaccc actgcacgag tgtttgatgt agaaagatta tcctttccag atggatgatc
     1981 ccgtgataaa acactcatcc tccctgccgg tgaagtgtca tggttaacgc catcgtattc
     2041 cccatggtat cccttccact ggaataggtc agggtgatga atatcgtatc agggtcagta
     2101 agcgttccct ttgcccatcc cccgggttga tcgataaaat aaatgctctt tttgtccgga
     2161 tcaaacatgc cggcgagggt ctggtgtgcc atggagccat ccggctggga aaatatttta
     2221 tatccctcga caatgtgccc atgctgcgac gtgaataccc aggtgttcac accgatcccg
     2281 acgcgctgga ttgtggtatt caggtagtac ccttccccgt catccgcctt ccaggtccct
     2341 gtgatatccg gatattccgc cgggagtgcc gttgtattcg catgtccagc ctggaccggg
     2401 gaaacagact gggtcaggtt ctcctgcaat gagccggtca ggcaccccgc accggacagc
     2461 catgctgcca gcactgcaaa gaggagaaac gattgcaggt aaagattcat gtgtcaggtt
     2521 caccagggtg acaccatata ccttttccag gtatcctgag ccggttcgct ttgggatagt
     2581 gaccctcaac agcaggaatc atgcgagtga ttcccgattc agccgaaatc cgggcggaaa
     2641 ttctcaccct ccggcagccc ggcacggtga atgcgaggtg agcgaatgtc gcaccaagct
     2701 cccggatgac accggcagag agaatgcggc attaccatcc gggagatgca tcgcctgaac
     2761 caaaccgtgt atccggtatt ttcgaccgga tagggaaaaa agagaggggg gggtttttca
     2821 ccctgccacc gggatctgca cctcggtaag gagttcctct gcaggcgtat tgtccgggtt
     2881 gttcaggtac acctgccggt cgggcccggc tggttcaagc ccattctctg ccgcatacct
     2941 gaacgctttc tcgaatgcga cagagaatcc ttcgtgctcg tagggtcccc ggtatacgac
     3001 cgagactgcc tttactgccg gaagtgtcct gacagtgatt cccgggtcag ataccaccac
     3061 cggcccgtgg attgggatcg ccatctcgat atcggcatcg gtctcgcggt actcgccgtc
     3121 gtagcagagc gagaggaagg ggcctgtgat cgtaacgcct ttcttctggt tttccggcga
     3181 aaagatgatc tccataagtg cagccgagac ctggccgcag acctcttcgt aagttcctgt
     3241 ccttcggcca gaaagcaccc tcatggccgg gatctctttt attactggtt ctgaaacgtt
     3301 catggcaaac aactccgaaa agtccctgtt ctcgataatg aaggacaaca ccttctccag
     3361 ccgtgcgatc tcggactgcg cctcatgttt tcttctctct accagtttcc ggaccgcatc
     3421 acggtcctgg gcagaatgtg cagtgaggat atccccaatc tcggaaagcc cgaacccaag
     3481 cgtgcagagc gtcctgatct tcagtgcaga ctcgacctgg gtaaccgtgt agtaccggta
     3541 tcctgtgaac cggtccttta ccgcaggaac aaggatgccc ttgctgtcat agtaacgaag
     3601 cgccttctgg gtgagatggg tgagggtcga gaacctcccg attgtaatcc gatcggacgg
     3661 catggtgcat cacatgaata tgtgttttca tgatatagag ggtctttctc ttccccgggt
     3721 gaaggtttgg ggagaaatat atcggggaag ggaacagtac ctcaagtgat gcatatcaat
     3781 ggggtgcggc tgctgcatga ccggtttccc acggactgca gttatccctt caacctggaa
     3841 attttccgga agactgattg gatcgggttt tttcggccca tcacgttctt tatcggggag
     3901 aacgggacag gaaagtccac gctgcttcgg gctatcgcaa aaaaatgcaa tatatctatc
     3961 tgggaggatg aaaggaggct taaattccat ttcaacaggt acgaggatga gatgttccgg
     4021 tacatcgatg tcgaatggag cgggaagcag gtggcgggat cgttcttttc atccgagatg
     4081 ttccgctact ttgcagagat ccttgacgaa tgggcaatcg ccgatcccgg gacactctcg
     4141 tattttggaa acgactcgct gaggaccagg tcccacgggc agtcacacat gatgttttat
     4201 accagcaggt accagaggga aggcctcttt cttctcgatg aacccgagaa tgcactgtct
     4261 ccacgcactc agatagaact cctgcacctg ctccggaaag ccacatcaag agacaacgtc
     4321 cagtttctca tcgcaacgca ctccccgatt cttctggctt atcccggtgc ggacatctac
     4381 agttttgaca ggaccccaat ccagaaggtt gggtacgagg agacggaata ttacagggca
     4441 taccgggatt ttctcaacca cagggagaag tacctggact ggtcctgann nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt acgacaacgc gtggttatcc tccacgatct
     4621 tcccgtcacg gagccggatg atccggtcga aatacttttt gtgccagtcc tcgtgtgaga
     4681 ccatgacgat cgtctggtgc atctccctgt tgatctcctc gaagagatcg agcaccctgc
     4741 gggagttctc ggtatcgagg ttcgcacagg gctcgtcggc aaaaaggatg tcgggcctgt
     4801 tcacgatggc acgggcgatg gcgacccgct gctgctcccc acccgagagt tccttcggaa
     4861 ggtgccatct ccggtcagag agcccgatac ggtcgaggat atcgaacgtg gccgttgtat
     4921 attcctcatc tgtccggtcg ctccggagca ttgccggaag gaatacattc tcggcaaccg
     4981 tcagttccgg gacgagggca tagtcctgga agacgtaccc gagcttgtag agccggaaca
     5041 tggttttttc gtaatcgttc agcgtgttca cgtcgatccc atcgagcgtg atctctccgc
     5101 tcgtcggaac atcgaggagc ccgagcatgt ggaggagcgt cgacttgcca ctccctgaag
     5161 ctcccatgat cccgacgaac tcaccccttc cgatggtgag ggagacacca tccagtgccc
     5221 tgaccccgac gtttcccatc tggtagattt tctggagacc cgcacattca atcatggcat
     5281 caggccctca tggacgactg tatctcttca cgcgagacct ggtacgcggg aacatacccg
     5341 gcgacgatcg cggaggccaa tagtatcagc gaattcgaga cgaggtccat cggtgtcagg
     5401 tacatgtagg cagaccattc gggggtcacg atcgggttgt aggcaaggta gagcgcgagc
     5461 agtaatgcaa tcgcgatccc gagaacgatc ccgaacagtg caagaatgat gacctggaac
     5521 ccgtagttgt gcatgatgac ctccttctgc accccgatgg cccgcaggat cccgatctgc
     5581 ttgcggctgt tgatggtctt gatcgtgatg acgatgaaca ggacgacggt cgtgatgatg
     5641 atgctcacga tgagggagac catgttgatg atgtcgaaac tctggagcac gctcccgatc
     5701 gctttcgaga ggagatcgtc cgtggtatga acctgcgggg agaccccgta actcatcagc
     5761 atgtccttga caaacttctc agagtacccc ggcttggtct tgatagtgat gtattctgct
     5821 ttgtcgaggg attcaccctc aacaatttcc atgtctgacc acgtaatgag tgccgagtta
     5881 tcggaagagg tccatcctgt cgaataaatc cctttcaccc ggtagtcctt tgtgtacccg
     5941 ttcccgaatt cgaccgtaat cgagtccccg acccgcaccc cgccaagcga ggagtagaat
     6001 tcatcctcct ggctgacagt cgcatcccca gcaatgatct ttccaatgat aatctcgccc
     6061 gtatcgcttt cacccaggta actgcccgcg atcatctttg tatgtatggg agagacggac
     6121 ttctcgtcgg tcggacgtat cgcccgtatc gaacttccca ggatacgctg ccggtacttg
     6181 agcgtcgcac ctttcgagta gtgcggggtt gcccgttcaa cgcccggcat actgttgatg
     6241 aggtcgagcg tagcatcgag gtccgtgatg tactgctccc ccgcctgcgg gctgacgagc
     6301 acgtttccga cctcatagtc gacgatctgc gtcttgatgg tctgcccaat ccctgcaaag
     6361 agtgacggaa ggaagatcat gttggtaaag cacatcccga caatcaggat tgtcaggatc
     6421 gcgcttgacc tgctccctct ccggagagac cgccaggcca ggaacagcgc tactttgagt
     6481 tcctttttac ccatgggagg gccccgttcc cattcttctt cggcaaatac caataccggt
     6541 aggcaatgaa gccggcgacg aggacgatga tcacgacgag gacaatcgtc ccggtgtaat
     6601 cctcgggggt cacttggagg gacatcggga acaccacggt atgggtccca aggtcgtcgg
     6661 tataggtgac ggtcacgtga tactcatacg tccccccgga acctccgtca agcatgaaca
     6721 aggccggggc atcgttgttc ggcttgatct ttcctatgaa tgactgccgg ttcccggtca
     6781 tgggaaggtc gatgactgcg gtcacctgct tcgcctctcc cgtgccggta ttctcgatcc
     6841 ggatggtgag atcgaagggg gtccctgcag tcaccctcct gggagtcgta tctacagaga
     6901 cgaacccgag ttccgcttta cccatgatcg ggacctcgat tgtctcctcc tggtgcctcg
     6961 cggtcccgtc cgggagcttg taatcgaacg caagcagaac tttctggagg ccaactgcta
     7021 cgaacctgtc ggtgatgaat tcgagggtga ctgtccggtt ctctcccccg ttcaggggtt
     7081 cgatggtgag ggtatttgga ccctttgaac caagcgacgt cgtggaggta gcggtggaaa
     7141 gaacgatatc gcttgccctg atttcccctg cgttcctgat gattaataca acagggaaac
     7201 tctctccggg attgatactc tcgggaagct ccttgtagat gaccagggtg gggctctgca
     7261 tcacatactg ggcggtgttc acgtttaccg gtactgggta gcgaacattc ttcccgccgt
     7321 ttatctcaac ccagacctcg ggaaagtaca ggccctcctt ttttggtgcc tggattgaga
     7381 aggtgacatc cagagactgc cccggcccaa tggctccgaa atggtggtac gatccgtcga
     7441 ttacgcggat ctcgcctccc atcaggctca cgctgtcgat gatggcgggt atgtccgtgc
     7501 tcttcgtggt ctggaactca ccagaaactg aaacacctga tgattcacgc agggatgccg
     7561 agctggcggt gttcttgaga gtgacctgga tcgtcccgta gccttccggt gcgaggactg
     7621 atggagtgac cttatagtct gtgatgatga ctgtcggctc tgctgcaaaa acatgcgtgc
     7681 agcacgctcc tgcgaagagc aggatgaaga ttcctgcgat gaaaagattg atgctccggt
     7741 tgttttcgta ctgcatgaga tccccccaac ctatcagggc cgatcctcgt tccttccccc
     7801 gatgtatcct ccatttcatg ggaaagaaac ttgtgatgga tcgaggttgc aactccaaga
     7861 ttaataatat tacttaaagt aaagttataa ttaccgcgag tatgctatta tttagcactg
     7921 gagattgagg gcgattggca cgcttatttc ctgaatatcg ggaagaggtc caaaagaaga
     7981 ttattgcaac ggctcatacc ctcttccacg aaaagggata ctcgaacacg aagatgtcag
     8041 agatcgcagc tgcgcttggt gtgaccaagc cgaccctcta ccactacttt gacaccaagg
     8101 agaaactttt cattgcagta gccgagcatg agaggaagat gctgcaggaa tttattatcg
     8161 aatccttcac tgaccgggac tttgttgcgg gtgcgacagc ctttttcgac accatcatgg
     8221 agggattcct gaaaaatatc gggcccgaga gcgtggccat catcacgcgg gatgatcacc
     8281 tcaggcaaat aatcctcgac gatcgggaga agttcatcga cgtgatcgca ggattccttg
     8341 cagaaaggaa agcgacaggg gagataaggg aggatgcaga tgtcaggatt cttgcatgca
     8401 cattcaatgc cctcttccag ggtctcctga tctatgcgat gcaagggatg agaactgatg
     8461 atcttcgcct ggtctggaac tcggctgtca aagggttgac acagaagggg tcgtaatccc
     8521 ggccttctct caccctggac acgcctgcat tgtcaactga aagggaagtg agtgcccccg
     8581 cagcagtccc ctcctgctat ttcagtgcat acgccgtccc tgccttctcc acctattcac
     8641 tagtagacac ttttttcctg ttgttcgccg gccggtactc gaaccggtac atgagtgaaa
     8701 tgactgccgg catgatgagg atcgcaccaa ggagggagaa cccgacagtg atgaccgtgg
     8761 tgatgccaaa gttcgagatg atgttgaact ccgagaggat cagggccgag aacccgaaca
     8821 ccgtcgtcag ccccgagaca gtgatggcag cgccgatttt ctgcacgctc gtctggattg
     8881 ccgaaaagag atcgaggccc cgtgccagtt cctcgtcgac cctctccatg atcaggacag
     8941 tatattcgga ggcaactcca atcgtcatcg aaccgagcac ggcggtaagt ggggtgtagt
     9001 cgaggcccag gagatacatg atcgcaccat tccaccctac gattgccacg atgggaagaa
     9061 gcggcgagac tgcatgaacc cgcctgtata ccacgaggag aaaggcgagg atgaatgcaa
     9121 agcccagcac agtcatgatc agcttcgaat cactgatatc gtccatcagc gcagcaaaca
     9181 tctcgatctg gccggtgaca cgggcggtca ccccgggcgg cggggagttc cacgcaacgt
     9241 ccctctcgat caactggacc agtgatcggg cctgggatat ctccatgtcg acagttgaga
     9301 actcgagcac cgtctcgatc ttcccgttca ggtaccggtc ttttgtgacg gcgggaatgc
     9361 ggtcaagcac ctgctggacc tcgccttcgg tcgtggggag gtttccgttg ttgtactgcg
     9421 caaggagggt ggcgatactg gtcacgccgg ttatcttgtc gttgtgctcg atctcgtact
     9481 ccccgaagtc ccgtatccat gtaagcgtat cggggttcag gaccgcttct cccgtcataa
     9541 tgacagggat ggtgctggtc gagcccatag ccctcgtgac cttgttcatg ttctggagcg
     9601 caggcatctc ggacggaaca aaagtcttct catcggcact gatggggaca tcgttgtcca
     9661 agttgaaccc gacgattgcc accatgaaga cgagcatgag gataggcacc gggttcttcg
     9721 caatctttaa cgcgactgct cccaggaacg catcatattt ttctatgaac gacccgcgtg
     9781 aaccctcctg gtggacaggg gtcgtatcgc agcccttcca cccgagttcg caggcggcga
     9841 catcgttgat cttccctttt ggttccctcg ggcaatacct tatcaggatg ccaaagaccg
     9901 ggacgatgat gagggcggca aggtagcacg atatcacccc gattgtacag gtgatcccga
     9961 agtcacccac catggggatg ggaccaaaga ccatcgcaat gaaccccatc gaagttgcga
    10021 gcatggcgat cagcacagac ggcccggtct tcgtgatcgt ggtggtgatc gcgtcaggaa
    10081 gagaagattt ccggacctcc tcgtcaagcc tcgagtggat ctggattgcg taatcgatgc
    10141 ctatcccgat gagcacagga aacgccccaa tcaccaccat gctgatggga attcctgcca
    10201 gacccatgaa cccgaacgtc aggatgagac ccgttgcaac gacaataacc ggcaacaaag
    10261 tatagcgcac gtggttgaag agggtcatca ccgctacgac catcagcacc atggcggcga
    10321 ggatgaggat tcccatcgac gaccccatct cctgctgcat ctcctgggag aaagcaggag
    10381 atcccgagag tatgacggtg acaccgggag gagggttcga gagcgagacg atgctccgga
    10441 tgttgttgag ggcctgctcc tgcctgtcgc tcgagatgcc cggctggagc gaaacgacgc
    10501 tgatcgtcat catcttcgaa gggatatagc gatcaacgac ttccggtggg gcctggctga
    10561 caatctggtt gatctcagcc gatgatcctg ggagcgtccc gccgttcccc tgcctcagga
    10621 gatcgactat gccggacacc cgctccacat attgctggtt tgcgacatcc gcctggagtc
    10681 gctcgatgta gtgcaggcat tcaacctgcg tgatatcgtc ggattcgacg atgatcatga
    10741 tggcgtcaga cccgtacgca tcggaatagt gggagaggag ggccccgcgg ggagtcgttt
    10801 tgtcaatata ggtatcatcg ccggtctcca tcgtcacgag ggtgaccccg aaaagcgcaa
    10861 tggcaaatac caggactgcc actcccgcaa caatacgggg atgggtggtg actgcatacg
    10921 ccaaccgttc gaacgggttt ttcattggaa aatgcctcca ccaggattac gagtcatgga
    10981 caggaatggg attcgcggga cagggtcatt cctggatgca aaaaaactcc ggatagcgga
    11041 acccgacaca gcccgcaggt ggtacatgga tattcggcaa ctgtgtatcc aagttgtcat
    11101 gttcactaca acagtacttt ctgattttcc accccataat gttattgcct tttgatacgg
    11161 gaaacaggat gaaatcgctg gtaaaaggct tttccttttc atggatggtg aaacccggga
    11221 ctctcttcgc gcgaaatgcc acacgcgcct tcttgctccc tccaattttc ttgaatgtgc
    11281 aggtaattgg gatagtcctc acatcgcaca tttttggcct gtatagcaga ccatccagtg
    11341 accgggtcga cactggcacg cagtttttta aaacccgatg ttacagaacg gcccggaacc
    11401 gtgggagaga acaaaggtgc ccgggttcga acgtgtgaaa tttcgggtca tcatcagttc
    11461 atatcagccg gtccccggca tgttcgcccg gcaggcagtc atagacctcc gtcaccttca
    11521 tcacgaggag cgacctgcag aaaaggcccg gttttgccaa tgcaacctct tttttcatcg
    11581 tctcgtagtc ttctccgctg gtcttcacct cgatgtcccc cttgaccttg aagcatcccg
    11641 tgataccggg accccatacg taaagacagg ccctgggatt ctcgaggacg tttttgatgg
    11701 tctccttcat gaactggttg ccgatccaga tcgtctcagg gtcacgggga tacactgccc
    11761 ccattggagc gacattcggt tcaccctcct ttgacgcagt tgcaagcggg aatatcttcc
    11821 ccgacatgaa tgcagtcatc atctcgggat tcatcattgc catggatggg gtgttgcacg
    11881 cagaggctga taaatgaggg gggggatgca actctccggg tgcggccggg tgaattttgc
    11941 gattagccca ttaagtaagg acggggaaaa tcccggcctg atctcagggt ttgtaaagga
    12001 agattttccc ccctcatcgt ttccgaagga acgtcccctt ccggtactcg tcgaatgcct
    12061 gccggagctc ttcctgcgtg ttcatcacga tgggaccacg ccatgcaatc ggatcatgat
    12121 ggggcattcc cgagagatag aggaaccttg ccccatcgct ccccgcgact acgtgcaccc
    12181 gcttcccttc ccgcgaaaag aggatcatgg tgcggtttcc catctccttc tcctgccgga
    12241 ggtcgaagtg gccggacccg ccgatcacgt aagccgccgc ggtatgctct gcgctcacct
    12301 cgtgggagaa cggcacatcg ggcccgagca taacatcaaa gaattcgggg aacccgacga
    12361 tatcctggac cgggccggtc acctcaccga tcgtcccgga gatgatcctg atgcgcactc
    12421 ctctttgtgg agagatgatc gggacgtcat tgccgggtac ctcctggtat cgggggtcga
    12481 tcatcttgtg gctcctgggc aggttcaccc agagctggaa cccgcccatc cgaccgtcaa
    12541 caggctttgg catctcctgg tggatgatgc c
//
LOCUS       DAOQYR010000066        12300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_5634_length_12300_cov_8.000735, whole genome shotgun sequence.
ACCESSION   DAOQYR010000066 DAOQYR010000000
VERSION     DAOQYR010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 12300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12300
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5634_length_12300_cov_8.000735"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PKM87_08330"
     CDS             <1..718
                     /locus_tag="PKM87_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30110.1"
                     /translation="EVEAVLPRSSREGDCPAEGIPGSPPRPDTEVQPALPPEQEAIQN
                     DPVRTDLAHPGGGTVLDLIREGWDRLVRFFSNGEEATATEEPETGTASVLAESSPSRI
                     LVEPISITSESEPKLQEEPVDLSRWNVTNYTESPFEKCGRLYIEDDDLVIRSDLDDRG
                     FCIPLVDVAEVLDGETVPVLLLANGTQVGVAKRSASGKAMNIWIDPFLYTSPLARVMD
                     VLDGRARKAAVFVGREEVEG"
     gene            complement(882..1769)
                     /locus_tag="PKM87_08335"
     CDS             complement(882..1769)
                     /locus_tag="PKM87_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30111.1"
                     /translation="MTYDGRAIANHEIDAKILADSLIGVSAAVEEANQLINGTSSEVF
                     IKVRSSFKPGSFEINLVQFLISDGIQAATNLITILGFAGIGSYGVLQVIKATKGGKII
                     KKQSVEGGYSETYFENCTNPIVLHDPVIQCYESEIIKDSVKRAINPLNYTGYDTIGFS
                     SGDNQPEIVTKEEYLPLVSPDIEPDEEGQILDTVIGEGIYGIAQTNLEGYEKGWKLIR
                     DGGIFFPVTILDKRFLNDVRNRKYRFCNGSAVRVKLRDITKRKKNIVHEYEVIEIIAL
                     YEPGSSIPKIQRKDQKELF"
     gene            2137..4578
                     /locus_tag="PKM87_08340"
     CDS             2137..4578
                     /locus_tag="PKM87_08340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNQ30112.1"
                     /translation="MAIKKSELYSSLWSSCDELRGGMDASQYKDYVLTLLFMKYVSDK
                     YSGKKNPLIEIPAGGSFADMVRLKGDKEIGEKINTIIGKLAEANHLKGVIDQADFNDE
                     DKLGRGKEMQDRLSKLVSIFDSLDFRSNRAEGDDLLGDAYEYLMRHFATESGKSKGQF
                     YTPAEVSRVIAKVVGVGPGTRQDQTIYDPTCGSGSLLLKAADETPRGITIYGQEMDNA
                     TWALARMNMILHGHEGAELWRGNTLSVPHFKNPDDSLKTFDFAVANPPFSSKAWTSGL
                     DPANDIFGRFEYGIPPAKNGDYAFLLHLIKSLKSTGKGAIILPHGVLFRGNREADIRR
                     NLIRHGLIKGIIGLPPNLFYGTGIPACIIVIDKENARTRSGIFMVDASKGFIKDGNKN
                     RLRSQDLHKIVDVFTRQAEVPRYSRMVPVAEIASLANDYNLNIPRYIDSSEPEDLHDL
                     DGHLNGGIPERDVDALQPYWDVFPSLRGELFQPNGRPGYLDTKVNGLQVKAAILVNPE
                     FSVYANRVVAVFDRWQETHKDFLMRFDRGGKPKELIHTLSEDLLVRFTDLPLLNPYDV
                     YQCQMDYWADVMHDDLDLVAADGWIEAAKPRGIIEDKDRKIKETPDLTIGRKKYKMDL
                     VPPGLVVARFFAGEQAAIEELEALQETAARELEEFVEEHSGEEGLLEDVTNEKGKVTK
                     TAVKDRLREIGKDPEFADEREALARCMELLEAESGAGKAVKDARGVLDKKALTRYSTL
                     TEDDVKTLVVEDKWFASIRAGIEGEVQRLTQRLAARVKELDERYARPLPELEREVETF
                     RAKVEEHLKRMGLSW"
     gene            4615..5583
                     /locus_tag="PKM87_08345"
     CDS             4615..5583
                     /locus_tag="PKM87_08345"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNQ30113.1"
                     /translation="MDAVELIPEEGQPTFDTLLFIALEDLDSSIILYHREHYPQSVFY
                     LQQAVEKAVKSLGLLFEIVSEDDLQGKIGHNPLRVYKRPLTKISEDLPALNQDLDTHP
                     EFSEMLQSSGIDLDELASSIKSLVHKFDEYISTVVDYNLSAEALSEHLFAIDFFNRGI
                     DKTEERIRTEGISDEVFAAKKEELRTQLESLFNSIHIPTEQKEAMRNGLDSFFAAFLP
                     NRESFEYLMYVVLKIVRIGQNLFYLSIITSPHATKSRYPEGNFNPLQFYTPGSPLIDT
                     LPELQEITRHTLEEMDVLYDLIFDPPNDVPGVINDARLLSPGDEVE"
     gene            5580..6926
                     /locus_tag="PKM87_08350"
     CDS             5580..6926
                     /locus_tag="PKM87_08350"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNQ30114.1"
                     /translation="MRGEGLKPGYKKTVVGVIPEDWVVRSLGELFSLKPGFAFSSKYF
                     SIMGPILLTPGNFRLNGGLYFDGRNTKRYSGKYAPDMVFHNGDMLIVMTDLTPDCNLL
                     GKPAFIQSDELILHNQRIGKLVIKRKDIDVGFLYCFLLSRQYLDLIKEKATGSTVRHT
                     SNTSIYSVLILLPPLPEQTAIAAALSDTDVFIGALETLIAKKRAIKQAAMQQLLTGKT
                     RLPGFAKSSGYKNTEVGVIPEDWGLCQLDNLTIRIGSGITPTGGERVYSLSGRPFIRS
                     QNVGWGHLILDDLAHITDEIHYTFPSSEIEEGDVLLNITGASIGRTTLADIRIKGGNV
                     NQHVCEIRTDQNKLNPKYLTYFLLSNNGQRQIDSYQAGGNRQGLNYNQIGSFYIAVPP
                     RPEQTAIATVLSDMDDEIAALEHRLGKGQQIKQGMMQQLLTGKVRLVTPQEAEVAA"
     gene            6923..8380
                     /locus_tag="PKM87_08355"
     CDS             6923..8380
                     /locus_tag="PKM87_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ30115.1"
                     /translation="MKPIRIFISSVQPEFAAERAALRDYLRGDALMRRFFEVFLFEDM
                     PAADRRPDNLYLDEVRRCDLYVGLFGYEYGSENADGISPTGQEFDLATTEGKYRLIYV
                     KGRDDKNRHPKMRSLIGKAQAGLIRKRFSTAAELVAGLYAALIDYLIEKQLVRSVPFD
                     AAPCMKATLADLDPERMRWFVRTARAIRQFPLDEDADATELLEHLNLLDDGRLTNAAV
                     LLFGKQPQRYLLSSEVKCAHFHGTSVAKPIPSYQIYKGTVFALVDQAIDFVLSKIALS
                     VGTREAGSQAPVAYEIPREVVAEAIVNAVAHRDYTSSGSVQVMLFSDRLEVWNPGALP
                     PSLTLEQLRSAHGSVPGNPLLAEPMYLTRYIERMGTGIGDMIRRCRDAGLPEPEFAIT
                     DGFQTIIRRVEARRPGEEPSEKTSEKTSEKTSEKILAMIRKQPEITIEEMADTLGIST
                     RAVEMQLAKLKESGKLQRIGPAKGGHWKVSGESDE"
     gene            8373..11462
                     /locus_tag="PKM87_08360"
     CDS             8373..11462
                     /locus_tag="PKM87_08360"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNQ30116.1"
                     /translation="MSSIGDREILTQQRVIKFFDDNLGYRYLGDWQDREENSNIEEAL
                     LTDWLKRQQYSEKTIGKVIFSLGKARAVSGSKTLYDANREVYGLLRYGVKVRPDVGEH
                     HQTVWLIDWNNPTNNDFAIAEEVTISGEHAKRPDIVLYVNGIAVGVLELKRSVVSVTE
                     GIRQNLDNQKKTFIRPFFATVQLVMAGNETEGMRYGVIETPEKYYLRWKESRTLPEAG
                     ENPLLRELGQLCSKDRLLEIVHDFIVFDAGTKKTCRHNQYFGVKAAQERVKRREGGII
                     WHTQGSGKSLIMVWLAKWIRENITNARVLLITDRTELDDQIEKTFLGVDEDIYRAQSG
                     SDLVRVLNTSEEWLICSLIHKFGSGEDISEKDIKDYVEEIRKHLPKDFSPKGEIFTFV
                     DECHRTQSGKLHQAMKALLPGTMLIGFTGTPLLHQDKQKSIEIFGPYIHTYKYDEAVH
                     DGVVLDLQYEARDIDQSITSQERIDQWFEIKTHGLTNLAKAQLKQRWGTMQKVLSAQD
                     RLEKIVADILLDMETRDRLRSGHGNAMLVSGSIYSACRFFEMFQRTDLAGKCAIITSY
                     KPSPADIRGEETGEGLTEKLRQYDIYRRMLAEYFDEPEDEAMYKVEKFEQEVKNRFIK
                     EPGQMKLLIVVDKLLTGFDAPPATYLYIDKQMHDHGLFQAICRVNRLDSEDKEYGYII
                     DYKDLFRSLEKSIKDYTGEAFSGYDKEDVIGLLKDRLEKGRERLEEAREAVKALCEPV
                     ESPYDTAAYLRYFCASDTTNPTELADNEPRRIALYKQVAALLRAYANLANEMRSAGYS
                     DAEADVIRAEVTHYENVREAVKLGSGDYIDMKMFEPAMRHLLDTYIRAEESVKISAFD
                     DLSLVQLIVDRGVDAVDLLPETIRKHPEAVTETIENNVRKLITDESPVNPKYYEKMSA
                     LLDALIRERRAQAIAYEEYLKRIVELTRRIRNQSGDPRYSRVLDTPAKRALYDNLGED
                     EAIVLAVHEAIVNTKQEGWMYNPIKTRMVRYAIEDVLKDEQKTDHILDIVKNQEEYT"
     gene            11463..12179
                     /locus_tag="PKM87_08365"
     CDS             11463..12179
                     /locus_tag="PKM87_08365"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNQ30117.1"
                     /translation="MHQIQVGGLKVDVVRKNIKNLHLGVYPPDGRIRIAAPLRVNDEA
                     IRLAIVSKLTWIKKQQSRFLAQERQSEREFIYRESHYFFGNRYLLNIIEQSGPSKVKL
                     QNKTRIDLYVPVGCNGSKREQVLIEWYRRELKALIPPLISKWEEIMGVTVEDWGVKKM
                     KTKWGSCNIQSRRIWLNLELAKKPVQCLEYVVVHEMVHLLERKHNDHFKGLMDEFLPQ
                     WKHYRNILNQAPLSHETWSY"
BASE COUNT         3304 a         3052 c         3204 g         2740 t
ORIGIN      
        1 cgaagtggaa gcggtattac ctcgctcatc gcgagaagga gattgcccgg cagaaggcat
       61 accgggcagc ccacccagac ctgatacgga agtacaaccg gcactaccac cggaacagga
      121 agcaatccaa aacgatccgg tcaggacaga ccttgctcat ccgggaggcg gtaccgtgct
      181 cgacctgatc cgcgaggggt gggaccggct cgtccggttc ttctccaacg gcgaggaggc
      241 gacagcgaca gaggagccgg agaccgggac cgcttcggtc ctggcggaga gctccccatc
      301 ccggatccta gtcgaaccca tctccatcac gtcggagagc gagccgaaat tgcaagagga
      361 gccggtcgac ctctcccgct ggaatgtaac gaattacaca gagtcgccgt tcgagaagtg
      421 tggccggctc tacatcgagg atgacgacct ggtgatccga tccgacctgg acgaccgcgg
      481 cttctgcatc ccgctcgtcg atgtggccga ggtcctggat ggcgaaaccg tgccggtcct
      541 gctgctggca aacggtactc aggtgggagt ggcgaagcgg tcggcgtcag ggaaggcgat
      601 gaacatttgg attgacccgt tcctgtacac gtcgccactg gcgagggtga tggatgtcct
      661 ggacgggagg gcgaggaaag cggcggtgtt tgtggggagg gaggaggtgg aggggtaaaa
      721 agatctatct tgtttccacg agccaacgaa tgaaattgca ataattaggg aatcaagaaa
      781 tttggggata gattcctggt aaattacaaa tatcactctg gatataactg gaaatttaca
      841 ggaaaagatg attgaattgg ctgcattaaa ataattcaat ttcagaacaa ttccttttga
      901 tcctttcttt gaatttttgg aatactgctt ccgggctcat ataatgcaat aatttcaata
      961 acctcatatt catgaacaat atttttcttc ctttttgtaa tatcacgaag ttttactcta
     1021 actgcactac cattgcaaaa acgatatttc ctatttctta catcatttaa aaatctttta
     1081 tctaaaattg taactggaaa aaaaattcct ccatctcgga taagcttcca ccctttttca
     1141 taaccctcta aattggtttg tgcaattcca taaatcccct ctccaatcac ggtatcaagg
     1201 atttgacctt cttcgtcagg ttcaatgtca ggggatacta aaggaagata ttcttccttg
     1261 gtaactatct ctggttggtt gtctcctgat gaaaaaccaa tcgtatcata tcctgtgtaa
     1321 tttaaaggat taattgctct tttaaccgaa tctttgatga tctcactttc atagcattgg
     1381 atcactggat cgtgaaggac tattggatta gtacaatttt caaaataagt ttcagaataa
     1441 cccccctcaa cactctgttt tttaataatc ttaccgcctt ttgtagcctt tattacttgg
     1501 agtaccccat aagatccgat tcctgcaaaa cctaatatcg taataagatt ggtggccgct
     1561 tgaataccat cagatattaa aaattggacc agattaattt cgaaggatcc tggcttaaaa
     1621 ctacttctaa cttttataaa aacttccgag ctcgttccat taataagttg gttggcttct
     1681 tctactgctg ctgaaacgcc gataagtgaa tccgccaata tcttagcgtc aatctcatgg
     1741 ttagcaatgg ctctcccatc atatgtcact ttaaaattga caatttctgg atcccgccac
     1801 ataatcaatt gttaattgga ttttactata tcgtagatat ttcggtgcgt tataatcatc
     1861 cgtgatggat cacccaccat aggaattggt tgatcaacct agtctgcagc agaaccacgg
     1921 tcaaataata gaattataat aaaatcgaca gatatttttg attctggcca tatcttactt
     1981 gcagtaaaaa tgagatttat gataaggttc atgcccgagg gtggaacatg caatgaagac
     2041 attcatcgta tacatcgaaa cggtgctccg attcgagaat gacatcttat catcatattc
     2101 tgctagtggc aaattcctca ttcaaagggg atgaagatgg caattaaaaa atccgaactc
     2161 tacagctcac tctggagcag ttgcgatgaa ctccgtggag gaatggatgc ctctcaatac
     2221 aaggactatg tccttacgct cctgttcatg aaatatgtct cggataagta ttcagggaaa
     2281 aaaaatcctc ttatcgagat acccgcgggc ggcagtttcg ccgatatggt gagattgaag
     2341 ggggataagg agatcggtga aaagattaac acgattattg gaaaactcgc cgaagccaac
     2401 cacctgaagg gggtcatcga tcaggccgat ttcaacgacg aggacaagct cgggcggggg
     2461 aaggagatgc aggaccggct gtccaagctc gtttctattt tcgacagcct ggacttccgg
     2521 agcaaccgtg cggaaggcga cgacctcctt ggtgacgcct acgagtacct gatgcgtcac
     2581 ttcgcgactg aatctggcaa gagcaagggg cagttttaca caccggccga ggtgtcgcgg
     2641 gttatcgcta aagtggtcgg ggttggtcct ggtactcggc aggaccagac catctacgat
     2701 ccgacctgcg gatcagggtc tcttctgctc aaggccgctg acgagacccc tcgagggatt
     2761 acgatttacg gccaggagat ggacaatgcc acctgggccc tggcccgtat gaacatgatc
     2821 ctccacggac acgaaggcgc tgaattatgg cgcgggaata ccctttccgt cccacatttc
     2881 aagaatccag acgacagctt gaagaccttc gatttcgcag ttgccaatcc gccgttttcc
     2941 tccaaggcat ggacgagcgg ccttgaccct gccaatgaca tatttgggag attcgagtac
     3001 ggcatccctc cggcaaaaaa cggggattac gccttcctcc tacacctgat aaaatccctg
     3061 aaaagcaccg ggaaaggtgc catcatcctc ccgcatgggg tgctcttccg tggaaaccgg
     3121 gaggccgata tcaggcggaa cctcatcaga cacggcctga tcaaggggat catcggatta
     3181 cctccgaatc ttttctacgg tacaggtatc ccggcctgta tcatcgtcat cgacaaggag
     3241 aacgcccgta cccgatctgg catcttcatg gtcgatgcct cgaaagggtt catcaaggat
     3301 ggaaacaaga accggctccg ctcgcaggac ctccataaga tcgtggatgt cttcaccagg
     3361 caggctgaag tgccacgata ctcccgcatg gtaccggtcg cggagatcgc gagcctggcc
     3421 aacgactaca acctgaacat tccgcgctac attgattcca gcgagcccga ggacctccat
     3481 gaccttgatg gacacctcaa tggcggtatc cccgagcggg atgtcgatgc cctgcagccc
     3541 tactgggatg tatttccctc tctgcggggc gaattgttcc agccgaacgg ccgaccggga
     3601 tatctggata ctaaggtcaa tgggctgcag gtaaaggcag ccatacttgt caacccggaa
     3661 ttttcggttt atgccaaccg ggtagttgca gtattcgacc ggtggcagga gacccacaaa
     3721 gattttctca tgagattcga ccgcggagga aaaccgaaag agttgattca taccctctcg
     3781 gaagatctgc ttgtcaggtt caccgacctg ccgttgctca acccctacga tgtataccag
     3841 tgccagatgg actactgggc ggacgtgatg cacgacgacc tcgatctcgt cgcagccgat
     3901 ggatggatcg aggcggctaa gccccgcggg atcatcgagg acaaggacag gaagatcaag
     3961 gagaccccag acctaaccat cggccgaaag aaatacaaga tggacctggt cccgccaggt
     4021 ctcgttgttg cccgattttt cgccggggaa caggctgcca tcgaggaact ggaagccctg
     4081 caggagactg ctgcccgcga gcttgaggag tttgtcgagg agcacagcgg ggaagagggg
     4141 ctgcttgagg acgtgacgaa cgagaaaggc aaggtgacga agaccgcggt gaaggaccgg
     4201 ctcagggaga tcggcaaaga cccggaattt gccgatgaac gcgaggccct ggcgcggtgt
     4261 atggagctcc tcgaggcgga gtccggggct gggaaagcgg tgaaggatgc ccgaggtgtg
     4321 ctggataaga aggctctcac ccgatactct accctcactg aagacgacgt caagacgctg
     4381 gtggtggagg acaagtggtt tgcatcgatt cgggccggga tcgagggcga ggtgcagcga
     4441 ctgacccagc ggctcgcggc ccgggtgaag gagctggacg agcggtatgc ccgcccgcta
     4501 ccggagctgg agcgggaagt ggagacgttc agagcgaagg tcgaggagca cctgaagagg
     4561 atggggctgt cgtggtgatg aggagggaat atcgatgaga ttgaggaccc agggatggat
     4621 gcggtagaac tcattcccga agaaggtcaa ccaacctttg atactttatt gttcatcgca
     4681 ctggaagacc ttgacagttc cataatcctg tatcacaggg agcactatcc ccagtccgtc
     4741 ttttatctcc agcaggcagt ggaaaaggca gtcaagtccc taggacttct ttttgaaata
     4801 gtatccgagg atgatttaca agggaagatc ggtcataatc ccttaagagt ctacaaaaga
     4861 ccacttacta aaatctcaga agatcttccc gctctgaatc aagacctgga tactcatcct
     4921 gaattcagtg agatgctcca gtcatcgggg atcgatttag atgaacttgc atcgtccatt
     4981 aaaagcctgg tgcataagtt cgatgaatat atcagtactg ttgtagatta taatctatcg
     5041 gcagaggcac tttcagagca tctttttgca atcgattttt tcaatagagg aattgataaa
     5101 acagaggaaa ggattcgtac tgagggaatc agtgatgagg tgtttgctgc gaaaaaggaa
     5161 gagttacgaa cacagcttga gtcactattc aattcaatac acatccccac cgaacaaaaa
     5221 gaagcgatgc gaaatggatt ggattcattc ttcgccgcgt ttctcccgaa cagggaatcc
     5281 tttgaatatt tgatgtatgt agtgctcaaa atagttcgaa taggacaaaa cctcttctat
     5341 ctctcgataa tcacctcacc tcatgcaacg aaatcgcgat accctgaagg taatttcaac
     5401 ccgcttcaat tttatacacc aggctccccg ctgattgaca cattacctga attgcaggag
     5461 attacacggc acaccctcga agagatggat gtattgtacg atctgatctt cgacccgccc
     5521 aatgatgtac ctggcgttat aaacgatgca cgattgctta gtcctggaga tgaggtcgaa
     5581 tgagagggga ggggttgaag ccgggataca agaagacggt agtgggggtg atcccggagg
     5641 attgggtggt tagatctctt ggtgaattat tctcactaaa gccagggttt gcattcagca
     5701 gtaaatattt ctccataatg ggtccaatat tgcttactcc tggtaacttt cggctcaacg
     5761 gaggcctcta tttcgatgga agaaatacga agcgatactc tggaaaatat gcacctgata
     5821 tggtttttca caatggcgac atgctaatcg taatgacaga ccttacccct gattgcaatc
     5881 ttcttggaaa gcctgcattt attcaatctg atgaattaat attacataac cagcgaattg
     5941 gaaaactagt gattaagcgt aaagacatcg acgtaggatt tctctattgt ttccttctct
     6001 ctcgccaata tctcgatctt atcaaagaaa aagcgaccgg ttcaacagtc agacatacat
     6061 caaacacgag tatttacagt gtccttatcc tcctcccccc cctccccgaa caaaccgcca
     6121 tcgccgccgc cctctccgat acagacgtgt tcatcggggc gctggagacg ctgattgcca
     6181 agaagcgggc catcaagcag gccgccatgc agcagctcct gaccgggaag acgaggttgc
     6241 cggggtttgc gaagagttct ggttataaga atacagaggt gggggtgatt ccggaggatt
     6301 ggggcttgtg ccagttagat aatcttacaa tccgtatcgg tagcggtatt acaccaacag
     6361 gcggggaaag agtttattct ttaagcgggc gtccattcat tcgtagccag aatgtaggtt
     6421 gggggcatct tattcttgat gatcttgcgc acattaccga tgagattcat tacacctttc
     6481 cctcaagtga aatcgaagaa ggagatgttc tattaaatat taccggggct tcaatagggc
     6541 ggactactct ggcagatatt cgaattaaag gaggcaatgt caatcagcat gtctgtgaaa
     6601 ttcgaacaga ccagaacaaa ttaaatccga agtatcttac ttatttctta ctttcaaaca
     6661 acgggcaaag acagattgat agctatcaag cgggagggaa tcggcagggt ttgaactata
     6721 atcagattgg atcattttac atcgcagtcc ctccccgccc tgaacaaacc gctatcgcca
     6781 ccgtcctctc cgacatggac gatgagatcg ccgccctcga acaccgcctc ggaaaaggtc
     6841 agcagatcaa gcagggcatg atgcagcagc tcctcaccgg gaaggtccgc ctggtaaccc
     6901 cgcaggaggc ggaggtagcg gcgtgaaacc gatccgcatc ttcatcagca gcgtgcagcc
     6961 cgagtttgca gcggagcggg cggcactccg cgactacctg cgaggcgatg ccctgatgcg
     7021 ccggttcttc gaggtcttcc tcttcgaaga catgcctgcc gccgaccggc gtcccgacaa
     7081 cctgtacctt gacgaggtcc gccgttgtga cctgtatgtc ggcctgttcg gatacgagta
     7141 cggctccgaa aacgccgacg gcatctctcc taccgggcag gaattcgacc tcgccacaac
     7201 ggaaggcaag taccggctca tctacgtcaa ggggagagat gacaaaaacc ggcacccgaa
     7261 gatgcgttcc ctcatcggca aggcgcaggc agggctcatc cggaaacggt tctctaccgc
     7321 cgcagagctc gttgccggac tctacgctgc actcattgac tacctcatcg agaagcagct
     7381 cgtcaggtcg gtcccgttcg atgcagcccc ctgcatgaaa gcaaccctcg ccgacctcga
     7441 cccggagcgg atgcggtggt tcgttcgcac cgcgagagcg atccggcagt tcccgcttga
     7501 tgaggatgca gatgccacgg aactgctcga acacctgaac ctgctcgacg atgggagact
     7561 gacgaatgcc gcggtcctgc tcttcgggaa acagccacag cggtacctcc tctcatcgga
     7621 agtcaaatgc gcccattttc acggcaccag cgtggcaaaa cccatcccct cctaccagat
     7681 ctacaagggc acagtctttg cgctggtgga tcaggccatc gattttgtgt taagcaagat
     7741 cgccctctcg gtcggcaccc gcgaggctgg ttctcaggcc ccggtggcct atgaaatccc
     7801 ccgggaggtg gtggcagaag cgatcgtcaa cgcggttgcc caccgggact acaccagcag
     7861 tggcagcgtt caggtcatgc tcttctccga ccgcctggag gtctggaacc ccggggctct
     7921 gcccccgtcg ctgaccctgg agcagctccg gtctgcacac ggttcggtcc ccggcaaccc
     7981 gctccttgca gaaccaatgt acctgacccg ttacattgaa cggatgggca ccgggatcgg
     8041 cgacatgatt cggaggtgcc gggacgccgg gttgcccgaa cctgaatttg cgatcacaga
     8101 cggcttccag acgatcatac gcagggtcga ggctcggcga cctggggaag aaccttcgga
     8161 gaaaacttcg gagaaaactt cggagaaaac ttcggagaaa atcctcgcca tgatccggaa
     8221 gcagccggag atcaccatag aggagatggc cgatacactg ggtataagta cccgggctgt
     8281 cgagatgcaa ttagcaaaac tgaaagaatc aggaaaactg caacgcatcg gcccggcaaa
     8341 gggaggccac tggaaagtct caggagaaag tgatgagtag catcggagac cgggagattc
     8401 taacgcaaca gcgggtcatc aaattcttcg acgataatct cggctaccgc taccttggcg
     8461 actggcagga ccgcgaagaa aacagcaata tcgaagaggc gcttcttacc gactggttaa
     8521 aacgccagca gtattctgaa aagaccatcg gcaaggtgat cttctcgctc ggtaaagcac
     8581 gagcagtgag cggtagtaag accctctacg atgccaaccg tgaagtatac gggcttcttc
     8641 gttacggggt aaaggtccgc cccgatgtgg gagagcacca ccagactgtc tggttgatcg
     8701 actggaataa tcctaccaat aacgattttg caatcgctga agaggtcact atctccgggg
     8761 aacacgctaa aaggccggat attgtcctct acgtcaacgg tatcgcggtt ggagtgctgg
     8821 aactgaaacg gtcagtcgta tcagtcaccg aaggaatccg ccagaacctc gacaatcaga
     8881 agaagacatt catccggccg ttcttcgcca cggtacagct tgtgatggca ggcaatgaga
     8941 ccgaggggat gcggtatggt gtgatcgaga ccccggaaaa atattatctt cgctggaagg
     9001 agagcaggac tctgccagaa gcaggagaaa atccactctt gcgggaactg gggcagctct
     9061 gtagtaaaga ccgcctgctc gaaatcgtcc atgattttat cgtctttgac gcagggacta
     9121 agaaaacctg ccggcacaac cagtatttcg gtgtcaaagc ggcacaggag cgggtcaaac
     9181 gccgggaagg agggatcata tggcatacac agggcagcgg aaaaagcctg atcatggtgt
     9241 ggctggcaaa gtggatccgc gagaacatca caaatgcccg tgtcctgctt atcaccgaca
     9301 gaactgagct tgacgatcag atcgagaaga catttttagg agttgatgag gacatttacc
     9361 gcgctcaaag cgggagtgac cttgtgcgag tgctcaacac tagcgaggaa tggctgatct
     9421 gctccctgat tcacaagttc ggaagtggag aggacatcag cgagaaggac atcaaggact
     9481 acgtggaaga gatcaggaaa cacctgccga aagacttctc tccgaagggg gagatcttca
     9541 cgttcgttga cgaatgtcat cgcacccagt caggcaaact gcaccaggca atgaaagcgc
     9601 tccttcctgg cacgatgctg atcggattta ccgggactcc tctcttacac caggacaagc
     9661 aaaagagcat cgagatcttc ggtccctaca tccataccta caagtatgat gaagcggttc
     9721 acgacggcgt cgtccttgac ctgcaatacg aggcccgtga tatcgaccag agcatcacat
     9781 cccaggaaag aatcgaccaa tggttcgaga tcaagaccca tgggttgacc aacctggcca
     9841 aggcacagct caagcagcgc tggggaacga tgcagaaagt gctcagtgcc caggaccggc
     9901 tggaaaagat tgtcgccgat atcctgcttg acatggagac acgagaccgc cttaggagtg
     9961 gtcatggaaa tgctatgctc gtctctggaa gcatttactc agcctgcagg ttcttcgaga
    10021 tgtttcagag aaccgatctg gctggaaaat gtgccatcat cacgtcctat aaaccatcac
    10081 cggctgacat caggggcgaa gagaccggtg agggattgac cgaaaagctc cggcaatacg
    10141 atatatatcg gaggatgctt gccgagtatt tcgatgaacc tgaagatgaa gcgatgtata
    10201 aggtcgagaa gttcgagcag gaagtgaaga accggttcat caaagaaccg gggcagatga
    10261 aactcctgat tgttgttgac aaactcctca ccggctttga cgcccctcct gcgacatacc
    10321 tatatatcga taagcaaatg cacgatcacg gcctctttca ggcaatctgc agggtgaacc
    10381 gcctcgatag tgaggacaaa gaatacggtt acatcatcga ctacaaggac ctcttcagat
    10441 cgctcgaaaa atcgatcaag gattacacgg gagaggcctt cagcgggtac gacaaggaag
    10501 atgtcatcgg gctgctcaaa gaccggctgg aaaaggggcg tgaacgactg gaggaggcaa
    10561 gggaagcggt gaaagcactc tgtgaaccgg tcgaatcccc ctacgatacg gcggcgtatc
    10621 tccgctattt ctgtgcgtct gataccacaa atccgactga attggcggat aacgagccaa
    10681 ggcggatagc actttacaag caagtggcag cgttgttgcg agcgtatgcg aacctcgcta
    10741 atgagatgcg gagtgccggg tattccgatg cggaggcaga tgtgatcagg gccgaagtca
    10801 ctcactacga gaatgtccgt gaagcggtca agctgggtag tggtgattat atcgacatga
    10861 agatgtttga acccgccatg cggcatctcc ttgataccta catcagggct gaagagagtg
    10921 tcaagatatc tgcattcgat gacctctccc tggtccagct tattgtggat cgaggcgtag
    10981 acgctgttga cctcttaccc gaaacaatac ggaaacatcc tgaagcagtg accgaaacga
    11041 tcgaaaacaa cgtccgcaaa ctgatcaccg atgaatcccc agttaacccg aaatattacg
    11101 agaagatgtc agcacttctt gatgcactta tcagagaacg ccgggctcag gcaatagcct
    11161 acgaagaata cctgaaaaga attgttgaat tgacacggcg gatcaggaac caatcaggcg
    11221 acccacggta ttcccgtgtt ctggacaccc cggcaaaacg cgccctgtac gacaatctcg
    11281 gagaagatga agcgatagtc cttgcggtcc acgaggccat tgtcaatacc aaacaggagg
    11341 gatggatgta taatcccatc aagaccagaa tggtcaggta tgcaattgag gacgttctca
    11401 aagacgagca aaaaaccgat catatcctgg acattgtgaa gaatcaggag gagtatacct
    11461 gaatgcacca gatacaggtg ggagggctca aagtcgatgt ggtacggaaa aatatcaaaa
    11521 atcttcatct cggagtgtat ccacctgatg gtcgtatacg catcgcagca ccactccggg
    11581 ttaatgacga agccattcga ttggcaatag tatcgaagtt aacatggatt aaaaaacaac
    11641 agagcagatt tttagctcag gaacgtcaga gtgagcggga attcatctat cgggaaagtc
    11701 actatttttt cgggaatcgg tacctgctca acatcatcga acaatcaggc ccttcaaaag
    11761 tgaaactgca gaataaaacc agaatcgacc tctatgttcc tgttggatgt aatggatcga
    11821 aacgcgagca ggttctcatt gagtggtacc gtcgggaact caaagcactg attcctcccc
    11881 tgattagcaa atgggaagaa attatggggg tgaccgtaga ggactggggt gtaaagaaaa
    11941 tgaagactaa atggggcagt tgcaacattc aatcccgacg aatttggctt aatctggaac
    12001 ttgcaaaaaa accagtccag tgcctggagt acgtggttgt ccatgagatg gtgcatctgc
    12061 ttgagcggaa acataatgac cattttaaag gattgatgga cgagtttttg ccacaatgga
    12121 agcattatcg aaatatattg aatcaggcac ccttaagcca tgaaacctgg tcatactgaa
    12181 aattcatttt aattcaaatc ttgtaaattc tatttctaaa accatttcaa gccccgttcc
    12241 cccacccccc tcaaacttta tcaccatcct ccaccccacc tgctcacagc cctcaatcaa
//
LOCUS       DAOQYR010000067        12293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_5638_length_12293_cov_9.627880, whole genome shotgun sequence.
ACCESSION   DAOQYR010000067 DAOQYR010000000
VERSION     DAOQYR010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 12293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12293
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5638_length_12293_cov_9.627880"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..1362)
                     /locus_tag="PKM87_08370"
     CDS             complement(166..1362)
                     /locus_tag="PKM87_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNQ30118.1"
                     /translation="MSKNTDISPVIGSYQDAFTTYVKIKNYPDGEEKFTHLGALETNL
                     LRIIEEISGTEPEDAVFLRIRESTYTLLADTRILRAFLDPDDAAATESVHDAVSAARK
                     ALNLAVKEKDLRYANLVRSAISFTLSNAFILSDPSIRQEIIDLLKAGIESFDKTAPLI
                     DAANRKGISLYNTGTVLKKEFGDIKNLYERAYFLEQALDSFNKAAECFLASGHTEFLK
                     RSMKISASLESEINTIKSDPAYTGPVKKQAARKEPEKPFMIPCPSCGILNPEQAKFCN
                     SCGTPISTVSTPTPPAPPAPLTCPGCGSPVIPGKKFCGKCGTPVSTVSTPPPPAPPAP
                     LTCPSCGSPVTPGKKFCGKCGTPVSASSTTPTPPPPAPLTCPGCGSPVTPGKKFCGKC
                     GTRIPP"
     gene            complement(1362..>2132)
                     /locus_tag="PKM87_08375"
     CDS             complement(1362..>2132)
                     /locus_tag="PKM87_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30119.1"
                     /translation="ETEETEPETEPKTEPETEPETEETEPELFEGETVPEKIIEGVEG
                     VKGTLDDLNDKAGEMMDFVKDNNFHNFSPETKKKLENLQKKIKDSSDMVNDFLSGIKD
                     NAEAVKDKIDTIKKLKKNLGEIMKNLDDLNKTIKDLPMDGRSKAMLNMIVGTADAAGR
                     GFDEVLRNTLGDTVADAVNAKELGKDVGEAVGKTAENVTVRQRQILSDRELAVDVMGN
                     KAFEEYEDMRKGQEEIDEYYRKKQAKADAEQKQRESYH"
     assembly_gap    2133..2232
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2233..3356)
                     /locus_tag="PKM87_08380"
     CDS             complement(<2233..3356)
                     /locus_tag="PKM87_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30120.1"
                     /translation="MPAPTPPAPSTGAPGTISGSVDEFKAGMMDLKTFRQVSKEVPLA
                     KRPQKWWDLLSIIGGVVAAVIWIIYSGMSPAGGGVDLLTVILMIAVPIALVWFRTSID
                     GLLLPVQPTRKKIPKFVLVGVGIVIPFLTAFILYNILGIANYPLIQANLIIGTLCSYV
                     LVRDPVTNARTQPPGNRGRPALTILVVLISLLIVPVLADDCARDPTRAEDCLRTPGYA
                     ELISGTAAAGLSILVNGPIIIQTVAAGAGGGVSAGASAGAQTPPPTGYFENGQPYWGT
                     GNSNDPFRDYPDANNPPYMPSPDGKGPIWGDGTSGNPFRDYPDLPVYDHTPTPPDAGT
                     TPPATPPRKTLVTTPPSTSKSPPLTTPEPTSRTEPETEPET"
     gene            complement(3849..4883)
                     /locus_tag="PKM87_08385"
     CDS             complement(3849..4883)
                     /locus_tag="PKM87_08385"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNQ30121.1"
                     /translation="MKDPSKTRRHVTSAVQIVAGAALIIGLIIVLQFVTALIQEQNVP
                     EGWSIIRPPGEVSALVIDGDIVWTGGKDGLIPVNRTSGIQLPLPRGSPPFSHVRSLAI
                     DRQGWLWVAHDGGLVKYRNETWVVVSPGTGIPFTGAQAVLEDQEGAIWVGGEQVLATY
                     GNDTWQTVEPPGGWHIPSVDTLYQDREGVFWMGSVAPNTGGLYRFDAHSWTLFSIDDG
                     LPHNTIKEIAETPDGALWIGTGFSTRGGALRIQNGTWTRFYPDDGLAGNSTRSIYKDT
                     AGRMWFASEYDGVALYYEDRWGTLTTRDGLAGNEVKAIVEDQDGTWWLGTSGGLNRVE
                     RDSLFPGCNC"
     gene            complement(4880..5557)
                     /locus_tag="PKM87_08390"
     CDS             complement(4880..5557)
                     /locus_tag="PKM87_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ30122.1"
                     /translation="MIEIRNVSRHYGTVRVFSGLSLSIPDRTAVAITGPSGCGKTTLL
                     RLIAGLERPDSGEITIAGRLASSPDYLLAPSERSIGFVFQSPALWPHMTVRENILFGL
                     WNLPGSAREDRLIDLLNTAHISHLAHRYPHEISGGEAQRVALLRAIAPRPRHLLMDEP
                     LSNLDDDLREAMISLIITIMDQEDISLVYVSHRRDETDQIADRSYLFRDGALREVSEV
                     PAPGGSS"
     gene            complement(5554..7182)
                     /locus_tag="PKM87_08395"
     CDS             complement(5554..7182)
                     /locus_tag="PKM87_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNQ30123.1"
                     /translation="MMSHRPSCSLPLPEWPWILQAALLLIFLLLIYGPLSALLIDTLG
                     FIITDPDRALEILFLSSRRIGLLLQSIGLSAAVAGTVIAGGICAGSLIWQWRTGIGRY
                     IFWAALLLIPLPLTVHALTWSTLLASAGILPGLLAPQQSVHPWLISWLVESIAFLPLG
                     ICLSTLAFELVDPDRIEAARVLCPDPRVFSRIVLPLASPLLFAGAGFLFVFCLIDYTI
                     PSIYAANVYPFEIFAEFSTSYLPAAAFLLALPLLVVTVALVLLSQRPLKSVIQDSMWT
                     RREWQPEFLLPGWFVAAQRAALGIFFLQAVLVIAVLVVLAGTSADLATTLLLAGPDSL
                     TTVAVCLGATALSLPIALALASRLVEKKRISNAWWIAATIPLAIPPPLIGISMIGFWN
                     TPGDISLFGTLAMPVFAVMFRFLPLAALIIAAQLRAVNPLLLDAASIFGRSRVTNFLT
                     VYLPLILPGICIAAAGVFAFAIGELGATLLVIPPGHSTLTIRLYNYLHYGSSGSVAAL
                     GLMMTGIFLAIVSTGFLLVRRGLRTSRIHGDKPA"
     gene            complement(7188..8222)
                     /locus_tag="PKM87_08400"
     CDS             complement(7188..8222)
                     /locus_tag="PKM87_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNQ30124.1"
                     /translation="MKKVILPILVLVIVAAGILVFFALPMATQPKPEVVVYTSVDQVY
                     AEPILKEYEKISGVSVKAVYDVEATKTTGLVTRLISERANPQADVFWNGEIMQTILLK
                     ENEVLTPYTSPNSTGIPPLFKDPEGYWTGFGGRFRVIIVNTNLLSPEHYPDSIDDFRE
                     GTVPADQTGIAYPLFGTTATHAAALYATLGNQAARDYFIALRDRNVRVLDGNSVVRDQ
                     VASGLLAYGLTDSDDACGAVMRGDPVDIVIPDQGPGERGTLVIPNTVALIRGGPNPGE
                     GKKLIDYLLSPDTETELLDIGWIQMATRGNHPLRCVSTTSITPMNVSYPAIAAVQEEV
                     KRDLAEIFIR"
     gene            complement(8368..8817)
                     /locus_tag="PKM87_08405"
     CDS             complement(8368..8817)
                     /locus_tag="PKM87_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30125.1"
                     /translation="MPLIIGAGVVIVIIIAAIVIVPGLISPASVNGFLPGGTVPPTQT
                     FSLEPGPVDTVPSGNTVAVQVSRDPYTGIITSLFAGGPGQKVVQAIEITVMRQDGTTL
                     SGNIQPILLSEVQLQGTRRGVDRVIVRVTYESGETYKILDRQFSFND"
     gene            complement(9399..10202)
                     /locus_tag="PKM87_08410"
     CDS             complement(9399..10202)
                     /locus_tag="PKM87_08410"
                     /inference="COORDINATES: protein motif:HMM:NF022599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2927 domain-containing protein"
                     /protein_id="HNQ30126.1"
                     /translation="MPLPLIIGAGAAVVILIAAIAIVPGLISPEPANGSLPGGTIPPT
                     PTSSLEPGPVDTAPSETEVAVQVTRDTGISTEDINLHFMDVAFGGGNLFLERLPLSSK
                     RTTISLNAGEESDKVLIESFASNFNLLSQSNKLFENTKTGQTGDIRIKFLPSDGLKAI
                     DLTSDMKWLNREYTRDGITCAKIKGYDIYLNSDMEGDMRDHYLLRSLLYTLGFKGDSL
                     HYQDSIFAYPENTMTELSFLDEKTLQVMYGLGMNNGMTVEDVKRVLFFR"
     gene            10815..11990
                     /locus_tag="PKM87_08415"
     CDS             10815..11990
                     /locus_tag="PKM87_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNQ30127.1"
                     /translation="MALTAPPRFCTACGAPLSPGARFCEQCGQQVEEVVPVPFPVHIP
                     QIPAVIPFGTMKTGIFSFKDLVLVITADSLVAVVPVGTVAGELNRVQEEISATLEETG
                     IAARDFWEVSAHISPGLPRAYLTPRKVPVTLLNEVRSIRTRLGLDQAPWLRYARMTPA
                     EIMTESPDSRITARGEILYVRGEDQVADRYGEDLLVIRTRDREDRYRFSVGSYYPARS
                     TLISMLEHWQLPALPGEQIQSIVPACFEPGPKDFDFQYVFNLLFTDRRLILATTSGTD
                     EEVERQGTAYMEKVGQMATQQGMSPEAFGAASEWRDAPWQEFRQHSVHEILDSDGVNF
                     FIPHSILKGVSYKPGRRPALTLSLPEHTLTLEADPLFSPGPLRAAQASLRGVLSITI"
BASE COUNT         2685 a         3156 c         3564 g         2788 t
ORIGIN      
        1 gcggttgctc accacctggg atacgtaggg acgagggtcg aggtcgagac aaaaggcggg
       61 ccgcttgtaa tccaccttga cggggaggcg aagatggagg ggcctgcgac cacggtattt
      121 tcggggacga tcacgttctg aacctatatg ggataattga ttcactcacg ggggtatccg
      181 tgttccacac ttcccgcaga attttttacc aggagtgaca ggagagccgc agccgggaca
      241 ggtgagtgga gcgggtggag gaggggttgg ggttgttgaa gaagcgctta ccggtgttcc
      301 acattttccg cagaatttct tgccgggagt gacaggagag ccgcagctgg gacaggtgag
      361 tggagcgggt ggagcaggag gtggtggggt tgagacagtg cttaccggcg ttccacactt
      421 cccgcagaat ttcttgccgg ggatgacagg agagccacag ccgggacagg tgagtggagc
      481 gggtggagca ggaggggttg gggttgagac agtgcttatc ggcgttccgc aggaattgca
      541 gaattttgcc tgttccggat ttaatatccc gcaggatggg caggggatca taaagggctt
      601 ttcaggctcc tttcgggcag cctgtttttt gaccggccct gtataagcgg gatcgctctt
      661 tatggtgttt atctcggatt cgagggatgc agatattttc attgaacgtt tgaggaattc
      721 ggtatggccg gaggccaaaa agcattcggc agctttattg aaactgtcca acgcctgttc
      781 aaggaaataa gcccgttcgt acaggttttt gatatcgccg aactcctttt tcagcactgt
      841 accggtatta tacaggctga tgccttttct gttcgcggca tcgatcaagg gggccgtctt
      901 gtcaaacgac tctatgcctg ccttgagcag gtcgattatc tcctgcctga tggaggggtc
      961 tgacaggata aaagcatttg aaagggtaaa ggagatcgcc gagcggacaa gatttgcata
     1021 tctcaggtct ttttctttta ctgccaggtt caatgctttc cgagcagcag agactgcgtc
     1081 gtgcaccgac tctgtggcgg ctgcgtcgtc cgggtcaaga aaagcccgga ggatccgggt
     1141 atcggcgagc agggtatacg ttgattcgcg gattcttaag aagactgcat cttcaggttc
     1201 tgtccctgaa atctcctcaa taatccggag aaggttggtt tcgagcgccc ccagatgtgt
     1261 gaatttttcc tcgccgtcag ggtagttctt gatcttaacg taggtggtga aagcatcctg
     1321 gtatgatcca ataacgggag aaatatctgt attttttgac atcaatggta actctccctc
     1381 tgtttttgtt cggcatccgc tttcgcttgt ttctttcgat aatattcatc aatctcttcc
     1441 tgtccctttc tcatatcttc atattcctcg aatgctttgt tacccatcac atccacggcc
     1501 aattctctgt cgcttagtat ttggcgttgg cgcactgtga cgttttcagc tgttttccct
     1561 actgcttcac caacgtcctt gcctaactct tttgcattca cggcgtccgc aactgtgtcc
     1621 ccgagggtgt ttctcagcac ctcgtcgaat ccgcgcccgg cggcgtccgc ggtgcccaca
     1681 atcatattca gcatggcttt ggaacggccg tccataggga gatcctttat tgtcttgttc
     1741 aggtcatcga ggttcttcat tatctcgccg agatttttct tgagcttttt tattgtatcg
     1801 atcttgtctt tcactgcctc agcgttatct tttatcccgc tcagaaagtc gttgaccata
     1861 tccgaagagt ccttgatttt tttctgtaaa ttctcgagct ttttcttggt ctcgggagag
     1921 aagttgtgga aattgttgtc tttgacaaaa tccatcatct cgcctgcctt gtcgttaagg
     1981 tcatcgagag ttcccttgac gccctccacg ccctcgatta tcttttcggg gaccgtctca
     2041 ccttcgaata gttcaggctc agtctcttca gtctccggtt cagtctccgg ttcagtctta
     2101 ggttcagtct caggttcagt ctcttcggtc tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nngtctctgg ttcagtctcc ggttcggtcc gtgatgtcgg ctctggggtc
     2281 gttaacggcg gagatttcga tgtagatgga ggggttgtta ccagggtttt ccgtggcgga
     2341 gtcgccggtg gagtagtacc cgcatcgggc ggggttgggg tatggtcata tacgggtaaa
     2401 tccgggtaat ccctgaatgg gttaccactg gtcccgtccc cccatattgg tcctttccca
     2461 tctggcgatg gcatatacgg aggattgttc gcatccgggt agtccctgaa tggatcgtta
     2521 ctgttaccgg tcccccagta tggctgcccg ttctcgaagt acccggtcgg tggcggcgtc
     2581 tgtgcgcccg cactggctcc tgcacttaca cccccgcctg ctcctgccgc cacggtctgg
     2641 ataataatcg gtccgttgac gaggatcgag aggcctgcag ccgcggttcc cgatataagt
     2701 tcggcatacc caggtgttct taaacagtct tcggcccggg ttggatctct cgcgcaatca
     2761 tcggcgagga ctgggacgat caaaagggat atcagtacga ccagaatggt aagtgcgggt
     2821 ctgcctcggt ttccgggcgg ttgtgttctg gcattggtga ccgggtcacg gaccagtaca
     2881 taagagcaga gagttccgat aataaggttc gcctgaatca gcgggtagtt ggcaatcccg
     2941 aggatattat agaggataaa tgctgtcagg aacgggatga cgattcccac cccgaccagc
     3001 acgaatttag gtatcttttt acgtgttggc tggacaggaa gcagcaaccc gtcgatagaa
     3061 gtcctgaacc agaccagagc tatcgggaca gcaatcataa ggatgacggt caaaagatcg
     3121 acaccccctc ctgccgggct cattccggag tatatgatcc agatgactgc agcaacgaca
     3181 ccaccgataa tcgagaggag atcccaccac ttctgcggtc gctttgcgag cgggacctcc
     3241 ttactcacct gccggaatgt tttcaggtcc atcatgccag ccttgaattc atcgacactg
     3301 cctgagatcg tgccaggtgc cccggttgag ggggcgggtg gtgtaggtgc tggcaagatt
     3361 ccttgcttcc atgcggtgcc atcccagaag atccagccct gtccggaggg atcaggttgc
     3421 caccaggttc ctcgtgaatc ctgtacccga atctggttta ttccggcact gaattgttcg
     3481 cgggtgatgg agccattctg caacttccct ttgaggattg cgagtcgttt ctgtccttca
     3541 ggaatatcca ctatgaattg ccccctatga atgagtaggt aatccttaat ggggacacga
     3601 aattttatta atattcgcat attctttcag gcgacagcct gaaattccgg attaatttgc
     3661 gggaatctcc tagatagtcc acaagatgaa ttagttacag gttcgcagtg tttcttcctt
     3721 gtgatcctgg gatatctgga ttttaatgat atgccctgta agatttttat ttccgccaca
     3781 tactcatcca ggttcagatg acgaaaaacc actttacaga gaacgcagat ggttcaactc
     3841 acgattcgtt aacaattgca cccgggaaag agcgaatccc tctctacccg gttcaggccc
     3901 ccggaggtgc cgagccacca tgtaccgtcc tggtcttcta cgattgcttt tacctcgttc
     3961 ccggcaaggc catctcgtgt ggtcagggta ccccatcggt cttcataata cagtgcgacc
     4021 ccgtcatact cggatgcaaa ccacatccgc cctgccgtat ctttatagat agagcgggta
     4081 gaattcccgg caagaccgtc atcagggtaa aaccgggtcc acgtaccgtt ttgaatgcgc
     4141 aacgctccac cccgtgttga gaatccggtc cctatccaga gggctccatc cggggtttct
     4201 gctatttcct tgatggtgtt gtgcggaaga ccgtcatcga tagagaacag ggtccaggaa
     4261 tgagcatcaa accggtagag cccgccggta ttcggggcca ctgatcccat ccagaagact
     4321 ccctcccggt cctggtagag ggtatcgacc gagggtatat gccagcctcc ggggggttca
     4381 acggtctgcc aggtatcgtt tccgtatgtc gcaaggacct gttcgccacc gacccagatg
     4441 gctccttcct ggtcttccag gacagcctgc gcaccagtga atggaatacc tgtgccggga
     4501 gacacaacaa cccaggtttc atttctgtac ttcaccagtc ctccatcatg ggcaacccat
     4561 agccatccct gccggtcgat tgccagggaa cggacatggg agaacggagg agatccgcgc
     4621 gggagcggca gttgaattcc cgaggtccgg ttgacgggga tcagcccgtc cttgccgccg
     4681 gtccagacga tatcgccgtc aatgacgagt gctgaaactt cacccggcgg ccggataatg
     4741 ctccagccct ccgggacgtt ctgttcctgg ataagggcgg tgacaaactg gaggacaata
     4801 atgagcccga tgatgagtgc agcgccggca acgatctgga cagcagaggt tacatgacgc
     4861 ctggtcttcg acggatcctt catgaagatc ctccgggtgc gggcacttct gatacttccc
     4921 ggagtgcccc gtccctgaaa agataggagc ggtcggcaat ctgatccgtt tcatcccgcc
     4981 ggtgggagac atacacgagt gagatatcct cctggtccat gatggtgatg ataagtgaga
     5041 tcatcgcctc ccgcaggtca tcgtcaaggt tcgagagcgg ctcgtccatc aacaggtgcc
     5101 gtggccgggg ggcaatcgcc cgcaggagtg cgacgcgctg tgcctcgccc ccggatatct
     5161 cgtgcgggta acggtgggcg aggtgggaga tgtgtgccgt gttgagcagg tcgatcagac
     5221 ggtcttcacg ggcagaaccc gggaggttcc agagcccgaa gaggatattt tcgcggacgg
     5281 tcatatgcgg ccagagtgcg ggggactgga agacaaaacc gatcgatcgt tccgatggcg
     5341 ccagcaggta gtcaggtgaa cttgccagcc tgcccgcgat ggtgatctcc ccgctgtcag
     5401 ggcgttcgag tccggcaatg agccggagaa gcgttgtctt cccgcaccct gaagggccgg
     5461 taatggcaac cgctgtccgg tcaggtattg agagggaaag cccggaaaac accctgaccg
     5521 tcccatagtg tcgtgatacg ttgcggattt ctatcatgcg ggtttgtctc cgtggatacg
     5581 ggatgttcgc agtcctcgcc tgacaaggag aaatccggtg ctgacaatcg caaggaagat
     5641 gccggtcatc atcaggccaa gggcggcgac actcccggaa gagccgtaat ggagatagtt
     5701 ataaagccgg attgtgaggg ttgaatggcc cggagggatc acgagaaggg ttgcgccgag
     5761 ctctccgatg gcgaaggcaa agacacctgc tgcggcaata cagatgccgg ggaggataag
     5821 gggcaggtac acagtgagaa aattcgtgac acgactccgt ccgaaaatcg aagcagcgtc
     5881 aaggagcaac gggttcactg cacggagctg ggctgcaatg atgagtgcag ccagcgggag
     5941 aaaacggaac atgacggcga acaccggcat cgcaagtgtt ccaaacaggg agatatcccc
     6001 gggggtgttc cagaatccaa tcatgctgat cccgatgagc ggcggaggta tggcaagcgg
     6061 gatggttgcg gcaatccacc aggcattgga gatccgtttc ttctccacaa ggcggctcgc
     6121 gagggccagg gcaatcggga gggagagggc tgttgcacca aggcagactg caacagtggt
     6181 gagcgaatcc ggtccagcga gcaagagggt ggttgcaagg tcggcggaag ttcctgccag
     6241 caccacaagg acagcgatca ccagaacggc ctgaagaaag aaaatgccca gtgcagcccg
     6301 ctgtgctgcc acgaaccagc ccggcaataa gaattcaggc tgccattccc ttcgagtcca
     6361 catactgtcc tggataacgg acttgagcgg tcgctgcgag agcagcacga gggcaacggt
     6421 aaccacaagg agagggaggg caagaagaaa cgcggctgcc gggaggtaac tggtgctgaa
     6481 ctctgcaaag atctcgaaag gatagacgtt tgcggcatag atggacggga tggtgtaatc
     6541 gatcaggcag aacacaaaga ggaagccggc accggcaaag aggagcggcg aggcgagcgg
     6601 aaggactatt cgcgaaaaaa cccgtggatc agggcaaaga accctggcag cctcgatccg
     6661 gtcaggatcc accagttcga atgcaagggt ggagaggcag atcccgagcg ggaggaacgc
     6721 aatcgactcg acaagccagc tgataagcca gggatgcacc gattgctggg gagcgagaag
     6781 accgggcagt atgcccgcag atgcaaggag cgttgaccag gtgagcgcat gcacggtcaa
     6841 tggcaggggg atgagaagaa gagctgccca gaagatgtac ctgccgatac ctgtcctcca
     6901 ttgccatatc agcgaaccgg cacagatccc tcctgcaatg acagtcccgg cgacggccgc
     6961 ggaaaggccg atactttgca ggaggagccc gatccgccgg gatgaaagga acaggatctc
     7021 aagggcccgg tcaggatctg tgatgataaa gccgagcgtg tcgatgagaa gagcagagag
     7081 cgggccgtag ataaggagga gaaagatgag gaggagggca gcctggagaa tccagggcca
     7141 ttccgggagg ggcagggaac acgatgggcg gtggctcatc atcgtcgtta acggatgaat
     7201 atctcggcga ggtccctctt tacctcttcc tggaccgcag cgatcgccgg gtaggagaca
     7261 ttcatggggg tgatagatgt tgtggaaaca caccggagag gatggtttcc ccgcgtcgcc
     7321 atctggatcc agccgatatc cagcagttca gtctcggtat ccggtgagag gaggtaatcg
     7381 atcagttttt tcccttctcc ggggttcggg ccgccacgga tgagtgccac ggtattcggg
     7441 atcaccagag tccccctttc acccgggccc tgatcgggaa tcacgatgtc cacgggatcg
     7501 cctctcatta cggccccgca tgcatcatcc gaatcggtga gaccatatgc caggagcccg
     7561 gaagcaacct ggtccctgac gacagaattg ccatcgagaa ccctcacatt gcggtcacgg
     7621 agcgcgatga aataatcccg cgccgcctga tttccgagtg tcgcatagag ggcagcggcg
     7681 tgggttgcgg tcgtcccaaa aaggggatag gcgatgccgg tctgatcggc agggactgtt
     7741 ccttcccgga aatcatcgat cgaatccggg tagtgttccg gtgagaggag gttcgtattg
     7801 acaataataa cccggaacct gccgccaaat ccggtccagt atccttcagg gtccttgaag
     7861 agtggaggga tgccggtaga attgggagag gtatacggag tcagcacctc gttctccttc
     7921 agaagaatgg tctgcatgat ctcgccgttc cagaatacgt cggcctgggg atttgcgcgt
     7981 tccgaaatca ggcgggtgac cagtcccgtt gtcttcgtgg cttccacatc gtagacagct
     8041 ttgaccgata ctcccgatat cttctcgtat tcctttaata tgggttctgc atatacctgg
     8101 tcaacagagg tatacaccac tacttcgggc ttgggctggg ttgccatagg aagggcaaag
     8161 aacaccagga tgcctgctgc gacaatcacc agtaccagga tcggcagaat aacctttttc
     8221 atacccttgg atcagcagga gaaaatttaa caattctgat ttggtaatca cgacgaacga
     8281 gagtgtcgtt cagagatatg tcttcgatct cgccctggcc gtctcctcgt ggttctgtgg
     8341 atgaaaaaga tgggggtctt gaaaccatca gtcattgaaa ctgaactggc ggtccaggat
     8401 cttgtaggtc tctcctgatt cgtaagtcac tcgcacgatc accctatcga cccctctccg
     8461 tgtaccttgc agttgtactt ctgacaggag gatgggctga atattcccgg agagcgttgt
     8521 tccatcctgc cgcatgacag ttatctcaat cgcctgcaca accttctggc ccggaccccc
     8581 agcgaagagg gaggtgatta ttccggtata aggatccctg ctgacctgta cggcaaccgt
     8641 gtttccggat ggcacggtgt cgactgggcc aggctcaaga gaaaatgtct gtgtcggggg
     8701 aacggtccca cctggcagga atccgttgac agatgccggg gatatcaatc caggaacaat
     8761 gacaatcgca gcgatgatga tgacgatgac aaccccggca ccaatgataa gaggcaatgg
     8821 cagacttttt ttcgaagggg caggctcctt tttgggaggg gtcgcgggac ctggcacagg
     8881 tgcaggagcg cttgtcatcg gttgtggagg cggaggcgga ccttcagatg ggaccggtgc
     8941 cccgcactct tcacaaaact gcgtatcacc cacgagaggt gctccgcatt ttccacagaa
     9001 acgtgccata catcaacctt ggcataccgt ctaaaatgct tttcgaagat gatgcacaat
     9061 tgataatgaa aggatggtgg cgtggaggga tttatcttcc ggtactgctt tcagtttcgg
     9121 taactggcac agctcgaaga aaagacagga aggaccgaat tgcagcatct cgaaaatttg
     9181 cttcacaacc ccgtaatggc cgatgattcc agccgggtct tgtgtcagtc acgatgtatg
     9241 agagatagca gcaggcgatt caatgttcca gggaatctct cgtggagcca gcttcccgca
     9301 gaggtcatca gtgtcccgtg ccatgcccat cacttcgatg ccacctcatc agggtgagaa
     9361 gaagtgcata accattggaa aaaaggaggc aaagcagatc acctgaagaa caggacccgc
     9421 ttgacatctt cgacagtcat gccgttgttc atgccgaggc cgtacatcac ctggagggtt
     9481 ttctcgtcca ggaaggagag ctcggtcatg gtattctcgg gatacgcaaa gatgctgtcc
     9541 tggtagtgga gggagtcacc cttgaacccg agggtataga ggagtgaacg gaggaggtag
     9601 tggtcacgca tatccccctc catatctgaa ttcaggtaga tgtcataccc cttgattttg
     9661 gcgcaggtaa tcccgtcacg ggtgtactcc ctgttcagcc atttcatatc ggaagtgagg
     9721 tcgattgcct ttaacccatc ggatggaagg aactttatgc ggatatcacc ggtctgtccg
     9781 gtctttgtgt tctcaaagag cttgtttgac tgggagagga gattgaaatt tgacgcgaac
     9841 gattcaatga gaaccttatc ggattcttcc cctgcgttga gcgagatggt ggtcctcttt
     9901 gaggagaggg gaaggcgctc caggaagagg ttacctcccc cgaatgccac gtccatgaag
     9961 tggagattga tgtcttcggt cgagatcccg gtgtccctgg tgacctggac agcaacctcg
    10021 gtttctgacg gcgccgtgtc gacaggcccc ggctcgagag aagatgtcgg tgtcggggga
    10081 atggtcccgc ctggaaggga tccgttggca ggttccgggg atatcaatcc tggaacgatg
    10141 gcaatcgctg cgatgaggat aacgacagcg gccccggcac caatgataag aggcagcggc
    10201 aaaccttttt tcgacggggc cggccccttt ttgggagggg tcccaagacc aggtaagggt
    10261 gcaggagcgc ttgcattcgg ttgtgggggc ggagctgggg cttcagcggg taccggtgct
    10321 ccgcactcct cacaaaattg tgcattacct gcgagggatg ccccgcattt ttcacagaaa
    10381 cgtgccatac atcaaccttg gcatgacgtc tgaaatgctt ttcgaagatg atacgcagtg
    10441 aatatggaaa ggatagtggc gtggaggggc ttgtcttccg gtactgcgtt cagtttcgat
    10501 aactggcacc gcttgaagga aggacaggga ggaccgaaat gcagcatgtc aaacatttgc
    10561 ttcataaccc cgtaatggcc gatgattcca gccgggcttg gtatctgcca caagggcccg
    10621 gataattgcc agggcggata tccagcatac gaacgagtgg gaacacacgc atttagatac
    10681 cagcaccggc caagatacag attgagatct ctcatgtgtc ccgaattatc tccacttttg
    10741 gggtcggcgg cttccgtcag gattgttttc catacctatg tgcactggtc agaattggtc
    10801 cctgcccggg gtggatggca ttgacggcac ccccgagatt ctgcacggct tgcggggcac
    10861 ccctctcccc tggtgcccgg ttctgcgagc agtgcgggca gcaagtagag gaagttgttc
    10921 cggtcccctt tccggttcat atcccgcaaa tcccggccgt tatcccgttt gggaccatga
    10981 agaccgggat attctccttc aaagatcttg tcctggtgat caccgcagac tcccttgttg
    11041 ccgtagtccc ggtcggcaca gttgccgggg agctcaacag ggtccaggaa gagatttctg
    11101 cgacgcttga agagacagga atagcggccc gggacttctg ggaggtatca gcgcacatct
    11161 cgcccggtct cccccgtgca tacctcaccc cgcggaaggt gccggtcacg ctcctgaacg
    11221 aggtccgttc catccggacc cggctcggac tcgatcaggc tccgtggtta cggtatgcga
    11281 ggatgactcc ggcggagatc atgacagaga gcccggattc ccgcatcact gcccggggcg
    11341 agatcctcta tgttcgggga gaggaccagg tcgcggaccg atatggggaa gatctcctgg
    11401 tgatccgtac cagggaccgg gaagaccgct accgattctc cgtggggagc tactacccgg
    11461 ctcgctcgac gctcatctcc atgctcgaac actggcagct tccggctctg ccgggggaac
    11521 agatccagag catcgtcccg gcctgctttg agcccgggcc aaaagacttt gatttccagt
    11581 atgtcttcaa cctgctcttt accgaccggc ggctcatcct tgccaccact tcggggaccg
    11641 acgaagaggt cgaacgccaa gggactgcgt atatggaaaa ggttgggcaa atggcaacgc
    11701 agcagggaat gtcacctgaa gcgtttggcg cggcctcgga gtggcgggac gccccatggc
    11761 aggagttccg acagcattct gtccatgaga tccttgattc agacggtgta aactttttta
    11821 ttccccattc catcctgaaa ggagtatcct acaagccagg caggagaccg gcgctcaccc
    11881 tttccctgcc agaacatacc ctgactctgg aagcggatcc cctgtttagt cccggacctc
    11941 ttcgggctgc ccaggcttcg ctccgggggg ttctttccat cacgatctga atttttttaa
    12001 aaagatcctg caggaaatcg tttttttggg attttactac ccgaacgagg agaccgggca
    12061 ttatgctgac cgtcccgtca ccggcgaaga ggtcttcatt gttttgaaaa atcccggcac
    12121 accggcatga ttggcagaag gaaccacagg gatgctgatg gtgagagccg cgtggctatg
    12181 gagtatgact gccattatat ccgttcacca ccgtttttcc cggtaacgcg ctttaggggc
    12241 gtattttttc acgagtgttt tgacctgctc gaaattttcg ggggtgcagt aga
//
LOCUS       DAOQYR010000068        12243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_5662_length_12243_cov_8.124303, whole genome shotgun sequence.
ACCESSION   DAOQYR010000068 DAOQYR010000000
VERSION     DAOQYR010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 12243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12243
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5662_length_12243_cov_8.124303"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2870)
                     /locus_tag="PKM87_08420"
     CDS             complement(<1..2870)
                     /locus_tag="PKM87_08420"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ30128.1"
                     /translation="MTAGLLVLLLVLAQAVSAAPPPFARLTLNHSQTYRNMTVSELET
                     IDLMDLSLSRVTLHSTSGDWTDTKNLTLPSVNAIRRVFSRNTSTWHGNTLVILGALDS
                     TVSGSGMDTYTIFIQEKMPVVQCQVPGADGDLFLIKGNTTARNYNLSTLFESAERMYI
                     LHTKGIAGIEKTPAGWNTYEVGSPWSFGYPPDLGKILLENNISLTNFVLSSSHANSMS
                     SLFPPIHGEYLLSGFQYHPPSPGKVTTYAAWPVIILDGDNRLELVGKTPPYHYNKRTG
                     GDLALTFDNTANIHNLSYVLIREGETYDADVHVNMTALEESGGAVGLDLILSGNPFLS
                     ILKDTGLGQSDLKKVVSYSIQAVGNSTPPHSTHFSKINITPGYGTSGYALHASQVTIP
                     FDDLQGLLPGNFSIFALGTDANNTVVALDQDRISIGNSPLANFTASPTSGPAPLPVQF
                     TDLSSDSPTAWNWTFGDGGSSGAQNPNHTYTVPGLYNVTLTASNAFGSDTEQKVFYIN
                     VTPYLPPDADFFAIPTTGQAPLTVQFTDSSTGTITSHAWTFGDGGTSTGASPSHTYLS
                     AGTYTVSLNVTGPGGSDTKTKAGYITVTPPILQADFSGTPTAGFVPHFVQFTDLSTGP
                     HDTWSWNFGDGGTSTSPNPGHTYTSTGTYTVSLSVRMGSGTPSMRTRTNYITVFAVPP
                     PPVAQFTANVTSGDAPLHVQFTDLSSGSPHEWNWSFGDGGNSTERHPLHHYTSPGNYT
                     VSLTVRGLGGFDTETKASFIHVLAYPPVAEFTANVTAGDAPLTVQFTDLSSNDPTTWS
                     WTFGDGGTSSERHPVHVYLYSGNYTVSLTAGNAQGTGTKVRSGYISVTGITPPPPVLR
                     ADFAAAPTSGYAPLTVLFADTSTGNPVSWNWSFGDGTSSMQQHPSHVYNSAGAFTVSL
                     MISNGTADDTLTRSNYIIVYYRGGGGGGGGGGGGGTFLVTSTP"
     gene            3238..4140
                     /locus_tag="PKM87_08425"
     CDS             3238..4140
                     /locus_tag="PKM87_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30129.1"
                     /translation="MKRIRSILSIILVAILVQGVFALSITVEPESIERGDQVTVAIVD
                     LPDNSTFSMHIEGTFAATPGGTFSFETRNLVLPFALNDGSLSATLRNTDTNVLIVKKG
                     DTEVKKVGLSQNGVFSTTDSGSIPAGTYETISLGGTAAADATHIIASMTIQGRKSGPP
                     DSEITFIVDGVADGEVAITVSVDGGTALSRTIQVGSPVTITPTQTYYSSGGGGGRTSV
                     YATTATTAVTTTPTTTRTTTVTASPTGEETAAPAAVQTVGEVTVTHEPPVTSSPSPTA
                     TPLAAYLAPLGIALLLLIGSMRRR"
     gene            4147..5139
                     /locus_tag="PKM87_08430"
     CDS             4147..5139
                     /locus_tag="PKM87_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1616 domain-containing protein"
                     /protein_id="HNQ30130.1"
                     /translation="MAEPGTVSPLGRFFSSFTLENTPADLVAVYLWTGLAALSIYAPV
                     IHETPLRVILALPMVLFFPGYALIAALFPGRGEIDGLERLALSFGLSIAVVPLIGLVL
                     NYTPFGIRLDPVVTSLVLFTLFMAAAAQYRRAGLPRGERFEVPFGEMWVSLRAEFFPR
                     EGSAVDRALSAILLFAIVAAVAATVYVVVVPKEGEKFTEFYILGKEGKAADYPTDLRT
                     GQPESIIIGVGNHEYRNVTYFVELHFVDQEFDPATNTSSVLSMERLLSFPVTLAHNTT
                     YQAQHSFLVNSTSGNQLKFLLFRDEVPPDSVWGAERINQSYRDLHLWVRVRQGS"
     gene            complement(5084..5896)
                     /locus_tag="PKM87_08435"
     CDS             complement(5084..5896)
                     /locus_tag="PKM87_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30131.1"
                     /translation="MKIRPHNDYALSGIVLLSAALVLLAIGAFTDRKDLSSATLFLSG
                     VACFLTGVFMLSFSRSDPLDPEIASLLPIAGMLNQCRICADLGVRGDAWFLPPSGEER
                     AIREFIPVQGLEIPESFPDFSFLTDPASPGILLTPSAYPLIDYLERKGGLQVPSTEPE
                     LLHGIGEICEDVLELAGKVVIERDGETLVMRVEGYRLFSGCLSIASHSPKCCTMHPCG
                     ICSLTACLLAMGLRTPWHLEHVRFEEGRRCLVVVFRSPAGPAPTGEGPGSSG"
     gene            complement(5893..6360)
                     /locus_tag="PKM87_08440"
     CDS             complement(5893..6360)
                     /locus_tag="PKM87_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30132.1"
                     /translation="MRAGLPLLCTLSVCATGSVIGAYGSRSFVLAVTAGAIAIVAGVL
                     VVYFRDSDPALFLMAQPCIFLSWDASPLLAFLFEAILAVALLFSTGLIQGKNDLSYCA
                     AFLLVMGITTLLLSGYTHVVRPLAAFIFAAGIACILVLGLAYHTMVRAGGEMQ"
     gene            complement(6360..7655)
                     /locus_tag="PKM87_08445"
     CDS             complement(6360..7655)
                     /locus_tag="PKM87_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNQ30133.1"
                     /translation="MDATRPTWGFIVAAIGTLICAWFLDSVPALFLSVFISGFVVFRA
                     LAFLRQAGATASTVSLEREIKPAWIRQGGVAAVSSIIHVRVPPGLSARVSDLPPPGAP
                     VVQGTAKSELVEGGEHRIVLRYGVACLSSGNILWKGMDLLVCDQFFSLSIPFRSEQFR
                     SPMLRVDPVGIYQKRDGLGLFGERDLEKVTVLKGSGVRSFRDYSPGDDPRSVDWKLSA
                     KFGKLFVREYAGLSGKHPLLVVDLPDNAVPCPPSLRDTVVGAALSAAREMSTGPHGCS
                     LMVISGANLLAFLPEERSLHRIERAILEFHSPQRAVQCFRSLDPVTAEAFRMRLAASA
                     SFHSSLEKRLLLIYSRFIPEIRPLPFDIQCARALCRRGEAALHVLTTGTGDISHLSTL
                     GLHARRLGVDASLGLPEAMASQDLIRRLRGTGYSVVRVV"
     gene            complement(7659..8627)
                     /locus_tag="PKM87_08450"
     CDS             complement(7659..8627)
                     /locus_tag="PKM87_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNQ30134.1"
                     /translation="MNNLSQDLSLLSGYYREICDRMGDFIVGNEPLIELCMIGLLSGG
                     HLLMEGTPGTGKTSMVKTLARLTGCEFARFQCAVDSQPADILGIRIYDQAKKDFTLRK
                     GPIFTNFLLIDEINRLAPKTQSAFIEAMSERQATLDGITTSIRSPFIVLATQNPFEFE
                     GTFPLIEAQKDRFMFSMTLSHLDSDGELEIIRREHSGRLDWDSYFTRLRPVMNSDVIQ
                     RLLSSVTRLHIEEPLLAYIRDLVMATRSHGDVHIGASSRASIALVRGSKALAALRERS
                     YVIPDDIKELAPRVFMHRIILTREAEISDITSSQVIDEVLERVEVP"
     gene            complement(8624..9514)
                     /locus_tag="PKM87_08455"
     CDS             complement(8624..9514)
                     /locus_tag="PKM87_08455"
                     /inference="COORDINATES: protein motif:HMM:NF025617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HNQ30135.1"
                     /translation="MPIISRPAWIAAFLVLFGLIAMLSHFSGTTLDYSRYNPEWNGTS
                     RFFEMIGEQDAVTITRFEDLPSHGDALLLMPAPEDEYREDEIAHLVEFLEGGNILVLV
                     SDREDENQITSALGSDIRIQHANLTSIDRYFDHPSSVIAFPAGDDPISAGIPHIVLNR
                     PSYLLGGVPVFESSLLSWVDADGDSRLGSEETLQRYTVIARDSVGEGTLYVIADPSIF
                     INGMLVAGPGDGNARLADNIITARPFLIVDQGHSRTAYAPEMIRVVNLFKESTITKMA
                     LITVIFLAASLLVIPWRRKS"
     gene            complement(9501..11285)
                     /locus_tag="PKM87_08460"
     CDS             complement(9501..11285)
                     /locus_tag="PKM87_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30136.1"
                     /translation="MRRLPAVLVSALLVIGCLVLIGMQAGSPLLYSPRSDPGVPDHVN
                     PAAQDMVTGDRAAQVLPLISDLLNTPGSLVLNIKGSDFEYAARDLQEYRQISRQLDSL
                     VINLDMTEGELNEFRKANQQNLAILTELFNGTGRWDELQTLEIRYRDSGDSQMLTSIT
                     YEGEALRRRIQDLYRDYLDQDQVMRETGEKFGLDTSSYDRSISDFREIVSRIDENQDE
                     RMVTFTPAATPGERLYQLTLTVERERVSYMDTAVIRGLLFVHEKGKPDEVDLFIDSRR
                     VRSISTNPDGSFSYSHLVEHDHEGTHTVFAVYSGSVFSEIRTFFVEREDTYLDLEPPV
                     PGEGRVELKGTLMAGSTPVRSAPIEILADGKRAGTTYTGEDGKFSIGLKLSSGNRKVK
                     VIFSADTFPLAPSESAVYEVLIPTPGPGTGNEESFLASPLHAAILAASVCASCVIAFF
                     YLHRRRPGPKAPAPVFHLPGEPVPDPNEAMQEVLAEGATPAGGDLARPGDERPPGVDA
                     PGDLPSLFSALRLAVSRHLAFLHPLSLTPRELCRISRDLAVQEAVCRFTNGYEQARYS
                     GAKVPEDELDVIAIAASSAMNSLEGADH"
     gene            complement(11282..11542)
                     /locus_tag="PKM87_08465"
     CDS             complement(11282..11542)
                     /locus_tag="PKM87_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30137.1"
                     /translation="MMKKHLAISALAGWVLLVIGFMVLARTLDLEIFFVLWLIGILII
                     VELVSDHAATPRYLRSQRLVIASGVILFGVIVARKILEILSK"
     gene            11673..12167
                     /locus_tag="PKM87_08470"
     CDS             11673..12167
                     /locus_tag="PKM87_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30138.1"
                     /translation="MKTISCLVAVMACLAVFTLPAAAMTSENLDISIGEDGNADIRFT
                     YSLNWIEYISVYLRMVDPAQELKKVLESNFHKPVQVIGVDSGSVHLSVDSFASVTSKD
                     GKTTVRTPALSFADGERILKTYWFAPLISVDLSPAVTTLRFPDGSKATFTDALEIPSQ
                     VHSW"
BASE COUNT         2563 a         3456 c         3723 g         2501 t
ORIGIN      
        1 ggtgtcgaag tgacaaggaa cgtcccgcca ccgccaccac ctccgccacc gccaccacct
       61 cccctgtagt agacgatgat gtagttgctc ctggtaagcg tatcatccgc tgtcccgttc
      121 gagatcataa gggagacggt aaaggcgcca gccgaattgt acacatgact gggatgttgc
      181 tgcatggagg aagtgccgtc accaaacgac cagttccacg agaccgggtt tccggtcgaa
      241 gtgtcggcaa agaggaccgt gagcggggca tacccggatg tcggtgccgc cgcgaaatct
      301 gccctgagca cgggcggcgg cggggtgatc cctgtcacgc tgatgtagcc tgacctcacc
      361 ttcgtacctg tcccctgggc attccctgcg gtaaggctca cggtatagtt gccagaatac
      421 aggtaaacgt ggaccgggtg cctctcgctg gaggtccctc cgtcgccgaa ggtccagctc
      481 caggtcgtcg gatcattgga tgagaggtca gtgaactgga ccgtgagcgg ggcatcgccg
      541 gctgttacgt tcgcggtgaa ttcagcgaca ggtggatatg ccagcacgtg gatgaaactg
      601 gccttggtct cggtatcgaa cccgccaagg cccctgacag tgagcgagac agtgtagttc
      661 cccggagatg tatagtggtg aagtgggtgc cgctctgtgc tgttcccgcc gtcgccaaac
      721 gaccagttcc attcgtgggg cgaaccgctg gagaggtcgg taaactgcac gtggagcggg
      781 gcatcgccgg atgtgacatt cgcggtgaac tgtgccaccg gcggtggcgg gactgcgaac
      841 acggtgatgt agttggtcct cgttctcatc gatggcgtgc cacttcccat cctcaccgaa
      901 agagagaccg tgtaggtccc ggtcgaggta tacgtatggc ccgggttcgg gctcgtggag
      961 gtcccgccgt caccgaagtt ccagctccag gtgtcatggg gccctgtgga gagatcggtg
     1021 aactggacga agtgggggac gaagccggcg gtgggagtcc cggagaagtc agcctggagg
     1081 atgggaggcg tcaccgtgat atacccggcc ttggtctttg tatcggagcc accggggccg
     1141 gtcacattca gggagaccgt gtaggtcccc gcagacaggt acgtatgtga aggactggca
     1201 ccggtcgaag tcccgccatc cccgaatgtc catgcatggg aggtgatggt ccccgtggaa
     1261 ctgtccgtga actggaccgt gagcggggcc tggcccgtgg tgggaattgc aaagaaatct
     1321 gcatcaggcg gaaggtacgg agtgacattg atgtagaaga ctttctgctc ggtgtcggac
     1381 ccgaatgcgt tcgatgcggt cagggtaacg ttgtagaggc ccggaacggt ataggtgtga
     1441 ttggggttct gtgcgccaga ggaaccccca tcgccaaagg tccagttcca tgccgtggga
     1501 gaatcgctgg agaggtcagt gaactggacc gggagggggg ccgggcccga tgtgggggag
     1561 gcggtaaagt tcgccagcgg ggaattcccg atcgaaatcc ggtcctggtc gagtgcgacg
     1621 acggtattgt tcgcatcagt cccgagcgcg aatatgctga agttgccggg gagcagcccc
     1681 tggaggtcat cgaagggaat agtcacctgg ctcgcatgga gcgcataccc ggatgtgcca
     1741 tagcctggcg tgatgttgat ctttgagaag tgggtggagt gcggaggcgt ggaattgccg
     1801 actgcctgga tggaatacga gactaccttc ttcaggtcgc tttgaccgag tcctgtatcc
     1861 ttcaaaatag aaaggaacgg gtttccggag aggatgaggt ccaggcccac cgcaccgccg
     1921 gactcctcga gcgccgtcat gttcacgtgc acgtcggcat cgtaggtctc gccttccctg
     1981 ataaggacat agctgaggtt gtggatattc gcggtgttat cgaaggtgag ggcaaggtcg
     2041 ccccccgtcc tcttgttgta gtgatatggg ggagttttgc cgaccagttc gaggcgattg
     2101 tctccgtcaa ggatgatgac aggccatgcc gcgtaggtgg tgacctttcc gggactggga
     2161 ggatgatatt ggaatccaga gaggagatat tcgccgtgga tgggtgggaa cagcgatgac
     2221 atgctgttcg cgtgggagga ggagagtaca aagttagtga gtgaaatatt attctcgagc
     2281 agtatttttc caagatctgg aggataccca aaactccagg gcgatccgac ctcgtatgta
     2341 ttccatcctg caggagtctt ctcgatacca gcgataccct tggtatgcaa tatgtacatc
     2401 cgttccgccg attcaaacag ggtcgagagg ttatagttcc tcgcggttgt gttgccctta
     2461 atcaggaaaa ggtccccgtc cgcaccgggg acctggcact ggacgaccgg catcttctcc
     2521 tggatgaata tggtataggt gtccatcccg ctgccggaca cagtcgagtc gagagcgccg
     2581 agtatgacaa gcgtatttcc gtgccaggtg gaagtgttgc gggaaaatac cctgcgaatg
     2641 gcattcacag aggggagtgt caggttcttt gtgtcggtcc agtcccctga cgttgagtgg
     2701 agcgtgaccc gggagaggga gaggtccatt aggtctattg tctccagttc agatacagtc
     2761 atgttcctgt acgtctggga atggttcagt gtgagccggg caaatggggg tggtgccgcg
     2821 ctgacggcct gggcaaggac aagcagcagc accaggagtc cggcagtcaa ccataatgcc
     2881 ctccatttat tcaatgcgcc atgacgacgc ttcgcgaacc ttgcgtgccg gggatatgat
     2941 acctctttcc cactctccat atctcgtttt ctcctcctac ccctgtatgc acctttggca
     3001 tgctgcacag ctttatatat atggtccttg tattaccctg catttaaatg tatggggaaa
     3061 aaacgcctgt tcccacgctg gatttcaaat gcggggaggc aatcagcggg aggtcatcat
     3121 caaggaattg tgcattatcc cgtgaggaca atacgttttg aacccggttc ctgacacacc
     3181 gaattgataa taagaaggcc cacaccatgt gaatataaaa gccaggggga ataactggtg
     3241 aaaagaatac gatcaatact cagtataatt ctcgtcgcaa tcctcgtaca gggggtcttt
     3301 gcactctcca ttactgttga accagagagc atcgagagag gagaccaggt caccgtcgca
     3361 atcgtcgacc ttccggataa cagcaccttc tcgatgcaca tcgaggggac ctttgctgca
     3421 acaccggggg gaaccttttc gttcgagacc aggaacctcg tgctcccctt cgccctgaat
     3481 gatggttctc tctccgcgac acttcggaat actgatacca acgtgctgat tgtaaagaag
     3541 ggcgacaccg aggtgaagaa ggtgggactc tcccagaacg gggtcttctc gacaaccgat
     3601 tccggttcta tcccggctgg cacctacgaa acgatctccc ttggggggac ggcagccgcg
     3661 gatgccaccc atatcattgc gagcatgacc atccagggga ggaagtccgg tcctcccgac
     3721 tcagagatca cgtttatagt tgacggggtt gccgatggcg aggtcgccat caccgtatct
     3781 gtggacggcg ggacggcact ctcccggacc atccaggtgg gaagccccgt gaccataacc
     3841 cccactcaaa catattactc cagtggcggc ggcggagggc gaacctcggt atacgcgacg
     3901 actgcaacca cggccgtgac caccacgccg acaactacgc ggacgacaac cgtgacggcc
     3961 tcccccacag gagaagagac cgctgcacct gctgcggtcc agactgtggg agaggtgaca
     4021 gtcacccatg aaccgccggt gacgtcttct ccctccccca ctgcaacacc acttgcggca
     4081 tacctcgccc cgctcgggat tgcccttctg ctcctcatcg gctcgatgag gcgccgctga
     4141 accaggatgg ctgaaccagg gacggtctcc ccgctgggga gattcttctc ctcgtttacc
     4201 ctggagaata cccccgctga cctcgtggca gtctacctgt ggacgggcct tgcggccctt
     4261 tccatatatg cccctgtcat acacgagacg ccacttcggg tgatcctggc cctccccatg
     4321 gtgctgtttt tcccgggata tgccctgatc gcagcgctct tccccggcag gggcgagatc
     4381 gacgggttgg agaggctggc actctcattc ggcctctcca ttgcggtggt tccccttatc
     4441 ggcctcgtgc tcaattacac accgtttggc atacgccttg accctgtggt gacctcgctt
     4501 gtgctcttca cccttttcat ggcggccgca gcacaataca ggagggcagg cctgccaagg
     4561 ggcgagcggt tcgaggtccc gtttggggag atgtgggtgt cgctccgggc ggagttcttc
     4621 ccaagagaag ggtcagcggt cgaccgcgcc ctctcggcaa tccttctttt cgccatcgtc
     4681 gccgcggtcg cggccaccgt gtacgtggtg gttgtcccaa aggagggaga gaagttcacg
     4741 gagttctaca tcctgggaaa ggagggaaaa gctgcagatt acccgacgga ccttcgcacc
     4801 gggcagccag aatcgattat catcggggtc gggaaccacg agtaccggaa cgtaacctac
     4861 ttcgtggagc tgcatttcgt ggaccaggag ttcgatcccg cgactaatac gagcagcgta
     4921 ctttcgatgg aaaggctgct ctcattcccg gtcacgcttg cacataacac cacctaccag
     4981 gcgcagcact cctttttggt caactccacg tccggcaacc agctcaagtt cctcctcttc
     5041 agggacgagg tgcctcccga cagtgtctgg ggggccgagc ggatcaacca gagctaccgg
     5101 gaccttcacc tgtgggtgcg ggtccggcag ggctcctgaa taccacgacg agacatctcc
     5161 tgccctcctc gaaacgcacg tgctccaggt gccacggggt acggagtccc atggcgagga
     5221 ggcaggccgt caggctgcag atgccgcagg gatgcatcgt gcagcacttg ggggaatgcg
     5281 acgcgatgga gaggcagcct gaaaacagcc tgtagccctc cactctcatg acaagagtct
     5341 ccccgtccct ctctatcacc accttccctg cgagctccag cacgtcctcg catatctcgc
     5401 ctatgccgtg caggagttca ggctcggtcg aaggaacctg cagtcccccc ttcctctcca
     5461 ggtagtcgat aagcgggtag gcggaagggg tgagcaggat gcccggggac gcggggtcgg
     5521 tgaggaacga gaagtccggg aacgactccg ggatctcgag gccctggacg ggaatgaact
     5581 cgcgtatcgc gcgctcttcg cctgagggag ggaggaacca ggcgtccccc cgcacgccca
     5641 ggtcggcgca gatgcggcac tggttcagca tccccgcaat ggggagcagt gaggcgatct
     5701 ccgggtccag cggatcggac cgggagaagc tgagcatgaa tactcctgtg aggaagcacg
     5761 caacacccga gaggaagagc gtggcgctgc tcaggtcttt gcggtcggtg aacgctccga
     5821 tcgccaggag cacgagcgcg gcagagagga gcacgatacc cgaaagcgcg tagtcgttgt
     5881 gggggcgtat cttcattgca tttcccctcc ggcgcggacc atggtgtggt acgcaaggcc
     5941 aaggaccagg atgcaggcga tacctgccgc aaagatgaag gcagcgaggg gacgcacgac
     6001 gtgggtgtat cctgagagga ggagagtcgt gatacccatt acgaggagga acgctgcgca
     6061 atacgaaaga tcattcttcc cctggataag gccagtggag aagaggagcg ccaccgcaag
     6121 gatcgcctcg aacaggaagg caaggagcgg tgaagcgtcc cacgagagaa agatacaggg
     6181 ctgtgccatg aggaagaggg ccgggtcgga atcgcggaaa tacacgacaa ggaccccggc
     6241 aacaatggcg attgctccag ccgtgaccgc gagcacaaag gagcgggatc cgtacgcacc
     6301 gatgacagaa ccggttgcgc atacagaaag cgtgcacaga agcggcaatc cggctctcat
     6361 cagaccaccc tgaccactga gtacccagtt ccccggagac gacggatgag gtcctggctt
     6421 gccattgctt cgggaaggcc gagacttgcg tccacgccca gccgccgtgc atgcaggcca
     6481 agcgtggaga gatgacttat atccccggta ccggtcgtaa ggacgtggag ggctgcctct
     6541 ccacgcctgc aaagagccct cgcacactgt atatcaaacg gcagtggccg gatctccggg
     6601 atgaaccggg agtatatcag gagaagcctt ttttccaggc tgctgtgaaa ggatgcactt
     6661 gctgcaaggc gcatgcggaa cgcttctgcc gtgacggggt caagcgaccg gaaacactgg
     6721 acggcacgct ggggagagtg gaactcgagg attgcccttt cgatcctgtg caaagatctc
     6781 tcttcgggga gaaatgcaag gaggtttgcc cctgaaatga ccatcaggct gcagccgtgc
     6841 gggccggtgc tcatctcccg cgcggcactg agtgctgccc ccacaaccgt gtcgcgcagg
     6901 gaaggcgggc acgggaccgc gttgtcgggg aggtccacga ccaggagagg atgtttcccg
     6961 gaaagcccgg cgtactcccg tacaaagagt tttccgaact ttgcagagag tttccagtca
     7021 acggagcgtg ggtcgtctcc cgggctgtaa tcgcggaatg accggacccc cgagcccttc
     7081 agcacagtga ccttctcgag gtccctctcc ccgaataatc cgagcccgtc cctcttctgg
     7141 tagatgccaa cagggtccac tctcagcatg ggcgatcgga actgttcact cctgaaggga
     7201 atggagaggg agaagaactg gtcgcatacc aggaggtcca tgcccttcca aaggatattt
     7261 ccgctcgaga gacaggccac cccgtaacga aggacaatgc gatgctctcc tccctccacc
     7321 agttcagatt tggctgtccc ctggactacc ggggccccgg gaggggggag gtcggacacc
     7381 cgcgcagaca gtcccggcgg gaccctgacg tggatgatgg aggacaccgc cgccaccccc
     7441 ccctgcctga tccatgcggg ctttatctcc cgttcgaggg atactgtcga ggctgtcgcc
     7501 ccggcctgcc tgagaaatgc gagtgccctg aacaccacaa accctgagat gaagacagag
     7561 aggaagaggg caggaaccga gtcaaggaac cacgcacaga tgagcgttcc gatggctgca
     7621 acgatgaatc cccatgtggg acgcgtcgca tccacggatc atggcacctc gacgcgttcc
     7681 agtacctcgt ctatcacctg ggaagaggtg atatcgctga tctcggcctc cctggtgagg
     7741 ataatccggt gcatgaagac tctcggggca agctccttga tgtcatcggg tatgacatag
     7801 gaccgttccc tgagcgcagc gagcgccttg ctcccccgga cgagggcgat ggatgcccgt
     7861 gagcttgccc cgatatgcac gtctccatgg ctccgtgtag ccatcacgag gtcgcggatg
     7921 taggcaagga ggggctcctc gatgtgcaac cgtgtaacag aagagagtaa tctctggatc
     7981 acgtcgctgt tcatgaccgg gcgaagcctt gtgaagtacg agtcccagtc aagccggccc
     8041 gagtgctctc tccggatgat ctcgagttcc ccatcactat ccaggtggga gagggtcatc
     8101 gagaacatga accggtcctt ctgcgcttcg atcaggggga aggttccctc gaactcgaat
     8161 gggttctggg ttgcaaggac gataaagggg gaccggatgg acgtggtgat accatcgagt
     8221 gtcgcctgcc gctcgctcat ggcctcgatg aacgcgctct gggtcttggg cgccaggcgg
     8281 ttgatctcgt ctatgaggag gaaattagtg aaaatcggac ccttgcggag ggtgaagtcc
     8341 ttcttggcct ggtcgtagat gcgaatcccc aggatatccg caggctggct gtccaccgcg
     8401 cactggaacc tggcaaactc gcacccggtc agcctggcaa gggtctttac catcgatgtc
     8461 tttcccgtgc cgggtgtccc ctccatcagg aggtgccccc ccgagagaag cccgatcatg
     8521 cagagttcga tgagtggctc gtttcctacg atgaagtccc ccatcctgtc gcatatctcc
     8581 cggtagtatc cggacaggag ggagaggtcc tggctgagat tgttcaagac ttgcgcctcc
     8641 acgggatgac caggagcgat gctgcgagga agatcacagt gatcagtgcc attttggtaa
     8701 tggtagattc cttgaacaga ttaaccactc ggatcatctc cggtgcatac gcagtccggg
     8761 agtgcccctg gtcgactatc aggaacggcc ttgcggtgat gatgttatcg gcaagtcggg
     8821 cattcccgtc accagggcct gcgacaagca tcccgttgat gaagatgctg ggatcggcaa
     8881 tcacgtagag tgtcccttcg cccacactgt ccctggcaat gaccgtgtat ctctggaggg
     8941 tctcttcgct gcccagccgg gagtctccat cggcatccac ccaggaaagg agcgagctct
     9001 cgaagaccgg cactcccccc aggaggtaag aggggcggtt cagtacgatg tggggtatcc
     9061 ccgcgcttat cgggtcgtcc ccggcaggga aggcgattac cgaagacggg tgatcgaagt
     9121 accggtctat gctggtgagg tttgcatgct ggatgcggat atcactccca agtgctgaag
     9181 tgatctggtt ttcgtcctcg cggtcagaca ccagcacaag gatatttccc ccttccagga
     9241 actcgacaag gtgtgcgatc tcgtcctcgc ggtattcgtc ttcgggggca ggcatgagga
     9301 ggagcgcatc accatgggag gggaggtctt caaagcgggt gattgtcacg gcatcctgct
     9361 ccccgatcat ctcaaagaag cgggatgttc cgttccactc tgggttatac cggctatagt
     9421 ccagggtggt ccccgagaag tgggagagca tggcaatcaa cccgaacagg accaggaagg
     9481 ccgcgatcca cgcaggacgg ctaatgatcg gcaccctcca gggaattcat cgcggagctg
     9541 gcagcaatgg caatcacgtc cagttcgtct tctggcacct tcgcaccgct gtacctggcc
     9601 tgctcgtagc cgttcgtgaa cctgcacaca gcttcctgga ccgcaagatc gcgcgagatc
     9661 ctgcagagct cccggggtgt gagggagagg gggtgcagga aggccaggtg ccgtgagact
     9721 gcaaggcgga gggcagagaa gagggacggg aggtccccgg gagcatcgac acccggcggc
     9781 ctttcgtctc ctggacgtgc caggtctcct ccggcaggtg tggcaccttc agcgagtacc
     9841 tcctgcatgg cttcattcgg gtcaggaact ggttcccctg ggaggtggaa tactggtgca
     9901 ggggccttgg gtcccggcct tcggcgatgg agatagaaga atgctatcac gcacgaggca
     9961 cacaccgatg ccgccaggat cgctgcatga aggggacttg cgaggaagct ctcttcgttc
    10021 ccggttccag gtccaggtgt ggggatgagt acttcgtata ctgcgctctc tgagggggca
    10081 agcgggaatg tatccgcgga gaagatcacc tttaccttgc ggttacccga agacagcttc
    10141 aacccgatgg aaaattttcc gtcttctccg gtatacgtgg ttcccgcacg cttaccatct
    10201 gcaaggatct ctatcggtgc ggacctgaca ggagtcgatc ctgccatgag ggtccccttc
    10261 aactccacac ggccctcccc tggaacgggg ggttcgagat cgagatacgt gtcctctctc
    10321 tcgacaaaaa atgtcctgat ctctgaaaag acagatcccg agtagaccgc gaagacagtg
    10381 tgagtccctt cgtggtcatg ctcgacaaga tgcgaatagg agaaggagcc gtcgggattt
    10441 gtggatatgc tgcggaccct gcgcgaatcg ataaacaaat ccacctcgtc aggctttcct
    10501 ttttcatgca caaagagcag tcccctaatg accgccgtgt ccatgtagga gaccctctct
    10561 ctttcaaccg tgagcgtgag ttgatacagc cgctcccccg gggtagctgc gggtgtgaat
    10621 gtgaccatcc tctcgtcctg gttttcgtcg atcctgctca cgatctcccg gaagtctgaa
    10681 atgctccggt catatgatga ggtatccaga ccgaacttct cccctgtctc tctcatcacc
    10741 tggtcctggt caaggtaatc cctgtagagg tcctggattc gccggcgcaa ggcttcacct
    10801 tcatacgtga tgctggtgag catctgggag tccccgctgt cacgataccg gatctcgagt
    10861 gtctgcagtt cgtcccaccg tccggtgcca ttgaaaagct cggtgaggat ggccaggttc
    10921 tgctggttgg ccttcctgaa ctcgttgagt tcgccctctg tcatgtccag gttgatgacg
    10981 agactgtcca gctgccttga gatctggcga tactcctgga ggtcccttgc ggcatattcg
    11041 aagtcactgc ccttgatgtt cagcacgagt gacccgggcg tattcagcag gtcggaaatc
    11101 agggggagca cctgtgctgc acggtcacct gtgaccatgt cttgggcggc cggattgacg
    11161 tggtccggca cccctggatc cgagcgcggg ctgtagagga ggggtgatcc ggcctgcatt
    11221 ccgatcagca ccaggcagcc gatcacgagc agtgcgctga cgaggactgc aggcaatcgt
    11281 ctcattttga caggatctcc agtatcttcc gggcaacgat caccccaaag aggatcaccc
    11341 cggaagcgat cacgagtctt tgggaccgca ggtaccgcgg ggttgctgcg tggtcagaga
    11401 cgagctcaac gatgatgaga atccctatca gccagagtac gaagaatatc tccaggtcga
    11461 gtgttcgtgc cagaaccatg aaaccgatga ccaggagcac ccaccctgcc agggcggaga
    11521 tggccaggtg tttcttcatc atggatatac tcctcttatg gagttttgtt gtaaagagga
    11581 tatcgtacag gattggtgca ggacatcttt cactaagaaa gcatttatgc tacggtcccc
    11641 gtaatactcc cgcatcaaag aggtgcattg acatgaagac gatctcgtgt ctcgttgccg
    11701 tcatggcctg cctggcagtg ttcactctgc cggctgcagc catgacctcg gaaaacctgg
    11761 acatctcaat cggagaggat ggcaatgcag acatccggtt cacctatagc ctcaactgga
    11821 ttgaatacat ctctgtatat ctccggatgg tggaccccgc ccaggaactc aagaaagtcc
    11881 ttgaatccaa cttccacaag cccgtccagg tcattggtgt ggacagcggg agcgtccacc
    11941 tctcggtgga ctcgtttgcg agtgtgacgt caaaggacgg aaagaccaca gtccgcacac
    12001 ccgccctctc ctttgccgat ggtgaacgga tactgaagac atactggttt gcccccctga
    12061 tcagcgtcga cctctctcct gcggtgacca ccctccgttt ccccgacggg tcgaaagcca
    12121 ccttcacaga cgccctcgaa attccctccc aggtccattc ctggtaagaa tacacctttt
    12181 ttcgaatgaa aatcacttcg tgattttcat cggttatgtt cgacagcagg gcgttcatga
    12241 gcg
//
LOCUS       DAOQYR010000069        12156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_5711_length_12156_cov_8.866871, whole genome shotgun sequence.
ACCESSION   DAOQYR010000069 DAOQYR010000000
VERSION     DAOQYR010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 12156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12156
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5711_length_12156_cov_8.866871"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..464
                     /locus_tag="PKM87_08475"
     CDS             30..464
                     /locus_tag="PKM87_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNQ30139.1"
                     /translation="METDRYVIKQISIFSENRPGRLAAIAKALTEENINILAFSIAEA
                     DGFGVVRALVDKPKLAYEKLTGLGFNVAFTDVIAVKMRESPGGLYEIAKMLANVQINI
                     EYSYAYTGKEGGVLILRVDDADQAVRLITSLGATLLESRVFQ"
     gene            complement(469..1755)
                     /locus_tag="PKM87_08480"
     CDS             complement(469..1755)
                     /locus_tag="PKM87_08480"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HNQ30140.1"
                     /translation="MKRPVLQVALDLLDLSRALPIAREAVRGGADWIEAGTPLIKSEG
                     MESVRRLRSAFPGHVIVADMKVADTGALEVEMAAKAGASVVCILGDADDAVIAEAVRT
                     ASVFGVRLMADLINVSDPVARSRELEALGVQILNAHVGIDQQMIGKSSLELLETIRGA
                     VGIEIAVAGGLDAQTAPQASARGADIVIVGGAITRSADVQDSARKVREALDSPVIGVL
                     PKVSVLEETRDLLSGVSAPNISDAMHRKGAMAGIIYLAGKKKMVGTAVTAQSFAGDWS
                     KPVEAIDHAHEGDVLVINNEGSTDIAAWGELATMSAETQGIEGVVIDGAVRDIDDIRK
                     MSLPVFARAVVPNAGEPKGFGEINCEVRCGGQTVRPGDWIVGDESGVVVIPKERAYEV
                     ARRAREVQKTEERVREEIRRGSTLSRVSDLVKWEKK"
     gene            1930..>2840
                     /locus_tag="PKM87_08485"
     CDS             1930..>2840
                     /locus_tag="PKM87_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HNQ30141.1"
                     /translation="MEILSIILTIAGLCLFETISSIDNAIINAEVLSTMAAWARRWFL
                     TWGLFFAVFVIRGLLPWLIIWMTTPSLGPIGALTATFSNDPAVIAAIEESAPILLMGG
                     GIFLVFLFLHWIFLEEKHYGLRGECYIASKGVWFFAVVSILLALIVWLALAVNPLMAF
                     AAVMGSTAFFIVHGFRQNAEEQERKMLGGTMSDWSKIFYLEVIDATFSIDGVLGAFAF
                     TLSVPLIIIGSGLGAIVVRQMTISNVDRIKRYLFLKNGAMYSILVLGSIMILDAFGFH
                     IPAWLSPIATFGIVGFFYVKSIRNMPPA"
     assembly_gap    2841..2940
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2941..3285)
                     /locus_tag="PKM87_08490"
     CDS             complement(2941..3285)
                     /locus_tag="PKM87_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNQ30142.1"
                     /translation="MPDKEGCRELSPRECSDLLSRDRERPCRILDVRTADEFRGGHLI
                     GAGHLDFYRPDFRQCIEQKDRTIRYLVYCRRGVRGQKTMELMHQCGFRDVVNIRGGYE
                     NWLSQGLPVQND"
     gene            complement(3384..3890)
                     /locus_tag="PKM87_08495"
     CDS             complement(3384..3890)
                     /locus_tag="PKM87_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNQ30143.1"
                     /translation="MNVGTTIIKCRHSVRSYKPVPVEDTIIRDAIECARFAPTAMNLQ
                     PWLIGVIRERDMLEKVAGLTDHGKFIADAPVCFAVFGERKAKYYLEDCCAATENLIIA
                     LQSYGVGSCWVAGDKKEYAEPVRRLLRVPDHYTLVSLLPAGYPADIALQKKKEQDEIV
                     FFDQYKED"
     gene            complement(4168..5139)
                     /locus_tag="PKM87_08500"
     CDS             complement(4168..5139)
                     /locus_tag="PKM87_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3821 domain-containing protein"
                     /protein_id="HNQ30144.1"
                     /translation="MNISSWYALIILSILLALSPVLAAENTVPVAGTVFLGEQGLDIS
                     ATGATAGSQIVYYGPGGSPANVPVATLTVDDPTEFYLSPSLFGGRTGPWFLSPANTLA
                     FYVQDPLIGIRVMDESADFAITSDVTWIPKGDVVGFRIDTNVYEIARRPGSPGAPITI
                     RVRGPGGVEYSSVTGYQLEDILISASPFDTGGVWLTGGFESGTYTVWAESTGNDMNLN
                     YKQEGKTVTVPVQFLLQSTNPLITPTPTTTPTTPATTTTTPHPTLPATTVTTLTPATV
                     VTESPATTPGTVIPTTVPPTTTPGFGTMLTLCAFFVACLLAVVRHQR"
     gene            complement(5185..6381)
                     /locus_tag="PKM87_08505"
     CDS             complement(5185..6381)
                     /locus_tag="PKM87_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNQ30145.1"
                     /translation="MAEIRIIGTAHVSKESVREVQEAIAEFSPDVVAVELDATRYAAL
                     KKQVQEASVESVLEAKTFTQLLVQWILAYLQRKIGLDVGVEPGAEMKAAVEEAEARHI
                     SIALIDRDIRITLRRFWHSLSFIEKIKMLYALVASVASIDEQEIDVEELKKQDVIAVA
                     MEEFRNFSPNGARALIDERDAYLAHQIRKLSLSHERILVVIGAGHKEGINRYLAHPET
                     LPSVESLTSEVKSRPWGLIFGGIVTFLFLFLLVAILFSGVGIDVLITALLYWVLIHGV
                     LTAVFTLLAKGHPLSALTGFAVSWFTALNPLIAAGWFSAIVEAKIRKPGPGDLGRISR
                     AESIGEMMEIPLFRVVIVAAMANIGSTLGTILYFVFIFPILGIDVGVVMTQGIANMWA
                     AIQAIF"
     gene            complement(6381..6914)
                     /locus_tag="PKM87_08510"
     CDS             complement(6381..6914)
                     /locus_tag="PKM87_08510"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNQ30146.1"
                     /translation="MRQEELAAEIRRIGFSCTRCGECCRETDTDTNLVMLYPQERAEL
                     CTATGREAEEFSEPYPEKVVMPGGGSIRFERCLRRREGVCIFLDGNRCRVYASRPWIC
                     RTYPFMLSGEDLIVFTCRGIGGTVSEGEAWEIAGRLLARNLAEKEEEERVRTLLSSNR
                     IPAGAEVLIDGTGMRIL"
     gene            complement(6922..7479)
                     /locus_tag="PKM87_08515"
     CDS             complement(6922..7479)
                     /locus_tag="PKM87_08515"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase subunit A"
                     /protein_id="HNQ30147.1"
                     /translation="MLPIYVVNNFGQFNHLILRMLRDRSVDARMISNSMPPEDVAAGC
                     RGLILGGGPDLSRSGNSARYLHLDLPVLGICLGLHVIAREFGGTVTKGRLGGYGPVEV
                     EIVEHDEILIGYPSRIRVWASHADEVTGLPAGAVRLAGSSVCGNEAVALPDRHIYGLQ
                     WHPEVSHTENGRYVYENFDRITREQ"
     gene            complement(7595..8605)
                     /locus_tag="PKM87_08520"
     CDS             complement(7595..8605)
                     /locus_tag="PKM87_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00303 family protein"
                     /protein_id="HNQ30148.1"
                     /translation="MAFVTGSPEIRAKNPLFCSILANTMLSVVPGISGAGPSPEKTLL
                     TPILDAELITCGKITSMPVRPDTPTGCPTPASITRAVMELCGLSPLFINGGLAHTPTV
                     PCLDTYSVPGEDPRFRDAVPDAGALFRKGRSIGTFLGSVSDLLVLGECVPGGTTTSLC
                     VLRGLGYDALVSSSYARNPVRMKEEVWSIVNRRIQEGDYRTPLDVIRCCGDPMIAIAA
                     GIAATYRGSIILGGGTQMLSVAAVLKGMNLPAPGIATTCYVRDDSSANFAEIAGMIGT
                     PVYYVDPGFGELGHSGLARYCIGEVKEGMGAGGAMFLAWLMGHSPDAIRKKILHTVHG
                     YA"
     gene            8694..9128
                     /locus_tag="PKM87_08525"
     CDS             8694..9128
                     /locus_tag="PKM87_08525"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNQ30149.1"
                     /translation="MFGIEGRLEERGISVDSMVTSAMLLYVPHGLSAEEAAHKVREKI
                     LKYLGDPNVASLLLGAILLEDELFLRQKDSGIQEDPVFLLSDEIIGMAIAECIGGTYA
                     RFEFTRYDQKKPGILATLGPFLDDAVAGLIAGCTSRLYSECC"
     gene            9119..9853
                     /gene="cobS"
                     /locus_tag="PKM87_08530"
     CDS             9119..9853
                     /gene="cobS"
                     /locus_tag="PKM87_08530"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNQ30150.1"
                     /translation="MLLNRLRALLQFTTILPLGRTVDFSLFADHTYLYPLSGYVIGGL
                     AGLAACWISIPEIRAAVALIALLCITGANHFDGLLDLGDGLMAHGGREGRIRALTDRQ
                     IGTGGFAAGSTVLLLTFAALSSLPEILWALVAAEVLAKFSMALLTAYGTPFHDGLHAY
                     LYHRAKPWFPVAAGICCLPLALLPLAYLPLSGAVLAALLIPLLLLLISERVFGGVNGD
                     VVGASGEITRAVVLCALALIPASALF"
     gene            9880..10791
                     /locus_tag="PKM87_08535"
     CDS             9880..10791
                     /locus_tag="PKM87_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ30151.1"
                     /translation="MLKDMGVHMRGGVITELDSEYCTIRTRIPAGVISCDQLKGIATV
                     AGRYGAESVHLTTRQTIEIPHMNHADLDAIARDLALNNTPVGSERDEVVNVIACPGNE
                     RCIFANIDSISLARSIDARLFGREMPVKIRISISACPNACTSPLLNEIGIVGQNRPIR
                     VPGMCTGCGTCVEYCKEEAITIKRGVSVLDPSRCVECGTCVRSCPYDLLKSSGEHYLI
                     TVGGRRGRHPRIGRELIAVKSPESVLLVVEKVVAWVYRRAWSGRLLSDQMDELQFETF
                     RDEIRALVPENDRAEETLGDFSGGSGI"
     gene            complement(10769..11071)
                     /locus_tag="PKM87_08540"
     CDS             complement(10769..11071)
                     /locus_tag="PKM87_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30152.1"
                     /translation="MKTLEITWDEKKYRATVECADGPVSVHRNCAFCLHCKGIRIGER
                     LYQSPQESVLRSFSSGGSSDEALMSAAMQFNTLVKDARAIECDDDADSGFKSRYRR"
     gene            11246..>12156
                     /locus_tag="PKM87_08545"
     CDS             11246..>12156
                     /locus_tag="PKM87_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 1-alpha"
                     /protein_id="HNQ30153.1"
                     /translation="MAVEKPHLNLAVIGHIDHGKSTTVGRLMFETGAVPPHIIEGYRK
                     EAETKGKATFEFAWVMDNLKEERERGITIDIAHKRFDTPKFYFTVVDCPGHRDFVKNM
                     ITGASQADAAVLVVAAPDGVMEQTKEHVFLSRTLGINQLIIAVNKMDAAKYDQKRFEE
                     VKKDLSALLQMVGYKPAETLFIPISSLQGVNIAKKSPETPWYTGPTLLESLDTLKEPE
                     KPTDKPLRLPIQDVYSISGIGTVPVGRVETGIMKKGMKVSFMPANKEGEIKSIEMHHE
                     EIPQALPGDNVGFNVRGLAKGDIRRGDV"
BASE COUNT         2566 a         3623 c         3294 g         2573 t
ORIGIN      
        1 aaacgggtga tcgacaggag gcagttataa tggaaacaga ccggtatgtg atcaaacaga
       61 tctcgatatt ttccgagaac agacccggcc ggctcgcagc aatcgcaaaa gccctcaccg
      121 aggagaatat caacatcctt gcattctcca tcgcagaggc ggacgggttc ggtgttgtca
      181 gggcgcttgt cgacaagcca aaactggcct atgaaaagct gacaggcctc ggattcaatg
      241 tcgcattcac cgatgtgatt gcggtaaaga tgcgggaatc ccccggagga ctgtacgaga
      301 ttgcaaagat gcttgccaac gtacagatca acatcgaata ctcgtatgcc tacaccggga
      361 aagagggagg agtcctgatc ctccgggtcg atgatgctga tcaggcagtc cggctcatca
      421 cctcgctcgg cgcaacgctc ctcgagagca gggtctttca gtaacccctt atttcttctc
      481 ccatttcacc aggtcggaga cccgggagag cgtactcccc ctcctgatct cctccctgac
      541 ccgctcctcg gtcttctgga cctccctcgc acgcctggca acttcgtacg cacgctcttt
      601 tgggataacg acaaccccgc tctcatcgcc gacaatccag tcgcccggcc ggacggtctg
      661 cccgccgcac ctgacctcac agttgatctc gccaaaccct tttggttcgc ctgcgttcgg
      721 gaccactgcc cgtgcaaata ccggcagtga catcttcctg atatcatcga tatccctgac
      781 agccccatcg atgaccacgc cttcgatccc ctgggtctct gcactcatgg tcgccagctc
      841 tccccatgcc gcgatatcgg tactcccctc gttgttgatc acgaggacat ccccttcgtg
      901 cgcatgatcg atggcctcaa ccggttttga ccagtcaccg gcaaacgact gggcggtgac
      961 cgcggtgccg accatctttt ttttcccggc aagatagatg atcccggcca tcgcaccttt
     1021 ccggtgcatc gcatctgaga tattcggggc tgaaaccccg gagaggaggt cccgggtctc
     1081 ctccagcacc gagacctttg gtagaactcc gattacaggg gaatccagcg cttcccggac
     1141 ctttcgtgcc gagtcctgca catccgcaga acgggtgatc gcacccccga caatcacgat
     1201 atcggcaccc cgtgccgatg cctggggggc agtctgtgca tcgaggccgc ccgcaaccgc
     1261 aatctcgatc cctaccgctc cgcggatcgt ttcgaggagc tcaagggagc tcttcccgat
     1321 catctgctgg tcgatcccca cgtgggcatt gaggatctgc actccaagag cctcaagttc
     1381 acgggaacgt gccaccgggt cgctcacatt gatcaggtcg gccatcagcc ggactccgaa
     1441 gaccgaggcg gtcctgaccg cttctgctat caccgcgtca tcagcatcac caaggataca
     1501 gaccaccgaa gccccggctt ttgctgccat ctccacctca agcgccccgg tatcggctac
     1561 tttcatgtca gcgacaataa catggccggg aaatgcagac cgcaatcgcc tgaccgactc
     1621 catcccctcg ctcttgatga gcggggtccc cgcctcgatc cagtcagcac cacccctgac
     1681 tgcctcccgg gctatcggca gagccctcga gaggtcaagg agatccaggg ctacctgcag
     1741 tacaggcctc ttcatgatca ctgaggtgag gagagagggc ataatagttt gccggatccc
     1801 cgtctgtgct ccccggtctt tgccgcgctt ccatggtaac acaataaaaa aaggtccgga
     1861 tcgtgccatt tccctgctcc atccgggaat gttttatggg aatcatcgca aacactctga
     1921 cgtacagcaa tggaaatcct gtcgatcatc ctgaccatcg cagggctctg cctctttgag
     1981 accatcagca gcatcgacaa tgccatcatc aatgctgagg tcctctccac catggccgca
     2041 tgggcgcggc gatggtttct gacgtggggg ctcttttttg cggtcttcgt catccggggg
     2101 ctcctcccct ggctgatcat ctggatgacg accccctcgc tcgggccgat cggggcactg
     2161 acggcaacat tctcaaatga tcctgccgtg attgccgcca tcgaagagtc ggcccccata
     2221 ctcctgatgg ggggtggcat cttcctggtg tttctcttcc tccactggat attcctggaa
     2281 gagaagcatt acggcctccg gggcgagtgc tatatcgcct caaagggggt ctggtttttt
     2341 gccgtcgtct cgatactgct tgccctcatc gtctggttgg cgcttgccgt caatccgctc
     2401 atggcatttg cggccgtcat gggctccacc gcattcttta tcgtgcacgg cttccggcag
     2461 aatgccgaag aacaggaacg gaagatgctc gggggcacga tgtcggactg gagcaagatc
     2521 ttttacctgg aggtgatcga tgccaccttc tcgatcgatg gcgttctcgg tgccttcgcc
     2581 ttcacactct ccgtgcccct catcatcatc gggagcggcc ttggtgccat tgttgtccgg
     2641 cagatgacca taagcaatgt cgaccggatc aaacggtatc tcttcctcaa gaacggggcc
     2701 atgtactcga tcctcgtgct tgggagcatc atgatcctcg atgcgttcgg gttccatatc
     2761 ccggcatggc tctctcccat tgccaccttc ggcatcgtgg gatttttcta tgtaaaatcg
     2821 atcaggaaca tgccgcctgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 tcagtcgttc tgcaccggga gcccctgcga cagccagttc tcataaccac cccgaatatt
     3001 caccacatcg cgaaaaccac actggtgcat cagctccatc gtcttttgtc cccttacccc
     3061 ccgtctgcaa tagaccaggt accggattgt ccgatccttc tgctcaatgc actgcctgaa
     3121 atcagggcga taaaagtcaa ggtgcccggc cccgatcaaa tgcccgccgc ggaactcgtc
     3181 agcagtcctg acatcgagga tgcggcaggg acgctcccta tcccgggaga gcaggtcaga
     3241 acattcccgc ggggagagtt cccgacaccc ttccttgtct ggcatagttc ttctctagca
     3301 ccctgggaaa gcccggaaac gggagaatcc cttctcatac gccctgtggc tggaaacgga
     3361 aagcgtctga atcccccgca tggtcagtct tctttgtact gatcaaagaa gacgatctca
     3421 tcctgttcct tcttcttctg gagagcgatg tctgccggat aaccagcggg caggagagag
     3481 acgagggtgt agtgatcagg aacccgcagc agtctccgga ccggctctgc gtactccttc
     3541 ttgtcaccgg caacccagca ggaccccacc ccataggact ggagggcgat gatcaggttc
     3601 tcggtggctg cacaacagtc ctcgaggtaa tactttgcct tccgttcacc aaatacggca
     3661 aaacagaccg gggcatcggc aatgaatttc ccgtgatcgg tcaggccggc aaccttctcc
     3721 agcatatctc tttcccggat gaccccgatc agccagggct ggagattcat cgcagtcggg
     3781 gcgaagcgtg cacattcgat ggcatccctg atgattgtgt cctcgaccgg aacgggcttg
     3841 taactccgga cactgtgccg gcatttgatg atggtggttc cgacattcat gagtatcccc
     3901 ttgcagtcaa gggaaaaaat ggttttggcc tgctgattcc cgccagggac agatcgatga
     3961 accgaggtct gtcacgatac tctgcaggca cagaaatcgg tagaggtcaa ttggatggca
     4021 ccagtacgct cggacttatt aaaacagcag gtgagagatc attttcccgg aaagagggag
     4081 ttgaagggaa agggatcgcc gtacctcagc gctgctgagg ggtttggcat tccatgatca
     4141 tctcccctga aaagggtggg attgttccta tctctggtgc ctgaccacgg ccaggaggca
     4201 ggcgacaaaa aaagcgcaga gggtgagcat ggtaccaaag cccggggtgg tcgtgggggg
     4261 taccgtggtc ggaataaccg taccgggcgt tgtggcagga gattctgtca caacggtggc
     4321 aggggtgagc gttgtcaccg tcgtagcagg gagggtagga tgaggggtag tggtggtcgt
     4381 ggccggcgtt gtcggcgttg tggtgggtgt tggggtgatg agcggattgg tgctctggag
     4441 aaggaactgc actggcacgg tcaccgtctt tccctcctgc ttgtaattga gattcatgtc
     4501 attcccggtg ctctccgccc ataccgtata ggtgccactc tcaaaaccgc ccgtcagcca
     4561 taccccccct gtatcgaatg gcgaggccga tatcaggata tcctcgagct gataccctgt
     4621 caccgaggaa tactcgacac cccctggacc ccggacgcgg atggtgatcg gagcacccgg
     4681 ggaaccaggc cgtctggcaa tctcatagac attggtatcg atccggaacc ccaccacgtc
     4741 ccccttcggg atccaggtga catcgctcgt gatcgcgaaa tcggcgctct catccatcac
     4801 ccggatcccg atcagcgggt cctggacata gaacgcaagg gtgtttgccg gcgacaggaa
     4861 ccaggggccg gtcctgcctc caaagagaga cggggagagg tagaattcgg tgggatcgtc
     4921 caccgtcagg gttgccacgg gaacatttgc agggctcccg cccggcccgt aatacacgat
     4981 ctgggaaccg gcagtggcgc cggtcgcgga gatatcaagc ccctgttctc cgaggaagac
     5041 cgttccggca accggcacgg tgttctctgc tgcgaggaca ggggagagtg caaggagtat
     5101 cgaaaggatg atcagggcat accaggacga aatattcata tgccactcat cgcagggaca
     5161 ctaaaaatag tttctgggag gcgatcagaa gatcgcctgg attgctgccc acatgttcgc
     5221 aatcccctgg gtcatcacca cgccgacatc gatcccgagt atcgggaaga tgaagacaaa
     5281 gtagaggatc gtcccaagcg tactcccgat atttgccatt gcggcaacaa tgaccaccct
     5341 gaagagcggg atctccatca tctctccgat cgactcagcc cgcgagatcc ggcccagatc
     5401 gcccggtccg ggtttccgga tcttcgcctc gacaatcgcg gagaaccagc cggctgcgat
     5461 cagcgggttt aatgccgtga accaggagac tgcaaatccg gtcagggctg aaagaggatg
     5521 ccccttagca aggagggtga atacggcggt aaggaccccg tggatcagca cccagtacag
     5581 gagggcagtg atcaggacat cgattcccac tcccgagaag aggatagcca ccaggaggaa
     5641 gaggaagagg aaggtgacga ttcccccgaa gatcagcccc cacggcctgg acttcacctc
     5701 actggtaaga ctttcgacgg agggcagcgt ctcagggtgt gcgagatacc ggtttatccc
     5761 ctccttgtgg cctgccccga tcaccacgag aatgcgttca tgggagaggg agagcttcct
     5821 gatctggtgg gcaagataag catcccgttc atcgatcagg gcccgggcgc cattcggtga
     5881 gaaattccgg aactcttcca tcgcgacggc aatcacgtcc tgtttcttca gctcctccac
     5941 atcgatctcc tgctcatcga tactggcaac ggaggcaacg agggcataga gcatcttgat
     6001 cttttcaata aacgagagcg aatgccagaa cctccgaagg gttatccgga tatcccggtc
     6061 gatcagggcg atactgatgt gccgggcttc ggcctcttcc acggcagctt tcatctctgc
     6121 ccccggctct acgccaacat caagcccgat cttccgctgc aggtaggcaa ggatccactg
     6181 gacaaggagc tgggtgaacg tctttgcctc gagcaccgat tcgaccgaag cttcctggac
     6241 ctgttttttc agggcggcat accgtgtggc atccagctcc acggcgacca catcaggaga
     6301 gaactcagcg attgcctcct gcacctcgcg gacactctct ttcgagacat gggcagttcc
     6361 gatgatccga atctcagcca tcagagtatc ctcatcccgg ttccatcgat cagcacttca
     6421 gcacctgcag ggatgcgatt cgaggagagc agggttcgaa cccgctcctc ctcctccttt
     6481 tcggccagat tccgtgcaag aagacgcccg gcgatttccc aggcttcacc ttctgagacg
     6541 gtgcccccga ttcccctgca cgtaaagacg ataagatcct ctccggaaag catgaacggg
     6601 taggtcctgc agatccatgg ccgtgacgca tacacccggc atctgtttcc gtcaaggaag
     6661 atacataccc cttccctccg cctgaggcat cgttcgaacc tgatactccc ccctcccggc
     6721 atcaccacct tctcagggta cggttccgag aattcttctg cctcccggcc agttgcagtg
     6781 cacagttccg ctctctcctg gggatacagc atcaccaggt tggtatccgt atcggtctcc
     6841 ctgcaacatt ctccgcaccg ggtgcaggag aacccgatcc tcctgatctc tgctgccagt
     6901 tcttcttgtc tcatctgctt ttcactgttc cctggtaatc cggtcaaaat tctcgtagac
     6961 atacctgccg ttctcggtat gactgacttc aggatgccac tgaaggccgt agatatggcg
     7021 atccggtaat gcaaccgctt cattgccaca gaccgatgag ccggcgagcc tgactgctcc
     7081 ggcgggaagg ccggtgacct catcggcatg ggaggcccat acccgtattc tcgacgggta
     7141 cccgatcagg atctcatcat gctcgacaat ctccacctcg acaggaccgt acccgcccag
     7201 cctgcccttg gtgaccgtac ccccgaattc ccgtgcgatg acatggagcc ccagacatat
     7261 tccgagcacc ggaagatcaa gatggaggta tcgtgcagag ttccccgaac gggagaggtc
     7321 aggtcctcct ccgagtatca ggccgcggca ccctgccgca acatcttcgg ggggcatgct
     7381 gttggagatc atccttgcat cgacagagcg atccctgagc atccgcagga tcaggtggtt
     7441 gaattgcccg aaattattga caacatatat cgggagcatc cctgatcact tctctctgat
     7501 ggctgataat ccctttttta tcggagcctt ccagaaatcc ccttccggcc ggcccgtcta
     7561 ttcccgtccc agacaatgta ccacagccct gttcctatgc ataaccgtgg accgtgtgga
     7621 ggatcttttt ccgtatcgcg tcaggagaat ggcccatcag ccaggcaagg aacatggctc
     7681 ctccggcacc catcccttcc ttcacctccc cgatgcagta ccgtgcaagc cccgaatgcc
     7741 ccagttcacc gaagccggga tcgacgtaat acacgggtgt ccctatcatg cctgctattt
     7801 cagcaaagtt ggccgaggag tcatccctta cataacaggt ggttgcgatc ccgggtgccg
     7861 ggagattcat ccctttgagg accgctgcaa cagagagcat ctgggtcccc ccgccgagga
     7921 tgatgctccc cctatacgtg gctgcgatcc cggcggcaat ggcgatcatc ggatccccgc
     7981 agcaacggat gacatcgagg ggggtacggt aatcgccttc ctgtatcctt ctgttcacaa
     8041 tgctccagac ctcctccttc atccggacag gattcctggc atagctgctg ctcaccaggg
     8101 catcataccc gagcccccgc agaacacaga gcgacgtggt ggtccctccg ggaacacatt
     8161 ccccgagaac cagaagatcc gaaacactcc caaggaaggt cccgatcgac ctccctttcc
     8221 tgaacaatgc accggcatca gggaccgcat ccctgaaccg gggatcttcg cccggtacgc
     8281 tataggtgtc gaggcatggt acggtggggg tatgggcaag ccctccgttg ataaaaaggg
     8341 gcgagagccc gcagagttcc ataaccgccc tcgtgatgga tgcaggcgta gggcagccgg
     8401 tcggagtatc gggccgcacc ggcatgctgg tgatcttccc gcaggtgatc agctccgcat
     8461 caaggatggg ggtgaggagc gtcttttcag ggcttggacc ggcacccgag atgccgggca
     8521 ccacagagag catcgtattg gcaagaatac tgcagaaaag aggattcttt gccctgatct
     8581 ccgggctgcc tgtcacaaat gccataagta ctcgatctaa cagctcacac cttccctgac
     8641 atcaaactat cctaacggca acctaatagc ggatgcagga acacatagca gatatgttcg
     8701 ggattgaggg acggctggag gagagaggga tctctgtcga ttccatggta acctctgcga
     8761 tgctgctcta tgtgccgcac gggctttctg cagaagaggc agcgcacaag gtacgggaga
     8821 agatcctcaa gtatcttggc gacccgaatg tcgcatccct cctcctcggg gccatcctcc
     8881 ttgaagatga actctttctc cggcagaagg actcgggaat ccaggaagac ccggtattcc
     8941 tcctctccga tgagatcatc gggatggcca tcgccgaatg catcggcggg acctatgccc
     9001 gtttcgaatt cacgcgttat gaccagaaga aacccggcat cctggcaacg ctcggcccgt
     9061 tcctcgatga cgcggttgcc gggctgatag ccggctgcac gtcgcggctc tacagcgaat
     9121 gctgctgaac cggctccggg ccctcctcca gttcacgacc atcctccctc tcggcaggac
     9181 ggttgatttc tccctctttg cagaccatac ctacctctat cccctctccg ggtacgtgat
     9241 cgggggtctt gccgggctcg cagcatgctg gatcagcatc ccggagatca gggcagcggt
     9301 tgccctcatc gcactgcttt gtatcaccgg ggcaaaccat tttgatggcc tcctcgatct
     9361 tggtgatggt ctcatggcac atggcggaag ggagggcagg atccgggcgc tcacagaccg
     9421 gcagatcggc accgggggat ttgctgcggg aagcaccgtc ctgctcctca ccttcgccgc
     9481 cctgtcttcc ctgcctgaga tcctgtgggc cctggttgca gcggaagtcc tggcaaaatt
     9541 ctccatggca ttgctgacgg catacgggac cccgtttcat gacggcctgc atgcatacct
     9601 gtatcaccgg gcaaagccct ggtttccggt cgccgccggg atatgctgcc tcccgctcgc
     9661 actcctcccg ctcgcatatc tccccctttc aggagcggtc cttgcagcgc tcctcatccc
     9721 gctcctcctc cttctcatct cagagcgggt attcgggggt gtcaatggcg atgtcgttgg
     9781 agcttccggg gagattaccc gtgcagtggt cctctgcgca ctggccctca ttcctgcgag
     9841 cgcacttttt tagggaatgc accgcgtaat aagttcctga tgctgaagga tatgggagtt
     9901 cacatgaggg gcggtgtcat caccgagctc gacagcgaat actgcacgat acgaacgcga
     9961 atcccggcag gcgtcatctc ctgtgaccag ctgaaaggga tcgccacagt ggccgggcgg
    10021 tacggggctg aatcggtcca tctgaccacc cgccagacca ttgagatacc gcatatgaac
    10081 catgccgacc tcgatgcgat tgcacgggat ctcgcactca ataatacccc agtcggctcc
    10141 gagcgcgatg aggtggtgaa cgtgatcgca tgcccgggga acgagcggtg catctttgcc
    10201 aacatcgact cgatatcgct tgcccgctcc atcgatgccc gactcttcgg aagggagatg
    10261 ccggtaaaga tacggatctc catatctgcc tgcccgaatg cctgcacgag cccgctctta
    10321 aatgagatag gcattgtcgg gcagaaccgt cccatcaggg tgcccgggat gtgcaccgga
    10381 tgcgggacgt gcgtggaata ctgcaaagaa gaggcgatca caataaagcg gggagtttcc
    10441 gttcttgatc cctcccggtg tgttgaatgc ggcacctgtg tcaggtcctg cccgtacgat
    10501 ctcctcaagt catccggtga gcattacctg atcacggtcg ggggccggag ggggaggcat
    10561 cccaggatcg gcagggagtt gatcgcggta aaaagtcctg aatcggtact cctcgttgtt
    10621 gagaaggtgg tagcctgggt ataccggagg gcatggagcg gcaggctcct ttcagaccag
    10681 atggatgagt tacaattcga aacattcagg gatgagatac gggcactggt ccccgagaac
    10741 gaccgcgcag aagaaaccct tggggatttc agcggcggta gcgggatttg aaccccgaat
    10801 ccgcatcatc atcgcattcg atggcccgtg catcctttac cagcgtgttg aactgcatcg
    10861 cggctgacat aagggcttca tcgctcgaac cacccgatga gaaggatcgc agtaccgatt
    10921 cctggggtga ctggtacagc cgctccccta tccggatacc cttacaatgc agacagaacg
    10981 cgcaattccg gtgaacggat accggcccgt ctgcgcattc gacagttgcg cggtattttt
    11041 tctcgtccca tgtgatttcc agcgttttca tgcgtgggca ggggttatct tgtccatatg
    11101 ttaaataatg tcgttttttt ccttttttct gaaaaaaccg acggatttcg ctcatcgaaa
    11161 cgattaataa ggatacgttt ccaataaata ctactcgcat aaacaggctg tacttctgca
    11221 gtctcgtttt tatcggagga tcattatggc tgttgaaaag ccccatctta atctggcggt
    11281 catcgggcac atcgaccatg gaaagtcaac caccgttggc aggcttatgt tcgagaccgg
    11341 tgccgtacct cctcacatca ttgagggtta ccgcaaagag gctgagacca agggtaaggc
    11401 aacctttgag tttgcctggg tgatggacaa cctcaaggaa gagcgtgaga gaggtatcac
    11461 gatcgatatc gcgcacaaga ggttcgacac cccgaagttc tacttcaccg tggtggactg
    11521 ccctggacac agggactttg tcaagaacat gatcaccggt gcctcgcagg cagatgcagc
    11581 ggttcttgtc gtggcagccc ctgacggggt catggaacag accaaggagc acgtgttcct
    11641 ttcaagaacg cttggtatca accagctcat cattgctgtc aacaagatgg acgccgccaa
    11701 gtatgaccag aaacgcttcg aggaagtgaa aaaggatctc tcagccctcc tccagatggt
    11761 tggatacaaa ccggctgaga cgctgtttat ccccatcagt tcccttcagg gggtcaatat
    11821 cgccaagaag agccctgaga ctccctggta taccggcccg acgcttctgg agtcgctcga
    11881 cacgctcaag gagcccgaga aaccgaccga caagccgctc cggctcccga tccaggatgt
    11941 ctacagtatc agcggtatcg gcaccgtgcc ggtaggacgc gttgagaccg gcatcatgaa
    12001 gaagggaatg aaagtatcct tcatgccggc aaacaaagag ggtgagatca agtccattga
    12061 gatgcaccac gaggagatcc ctcaggcttt gcccggcgac aacgttggat tcaacgtccg
    12121 tggccttgca aagggagaca tccgccgtgg cgatgt
//
LOCUS       DAOQYR010000070        11986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_5817_length_11986_cov_8.888358, whole genome shotgun sequence.
ACCESSION   DAOQYR010000070 DAOQYR010000000
VERSION     DAOQYR010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 11986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11986
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5817_length_11986_cov_8.888358"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..1103
                     /gene="carA"
                     /locus_tag="PKM87_08550"
     CDS             48..1103
                     /gene="carA"
                     /locus_tag="PKM87_08550"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNQ30154.1"
                     /translation="MKAVLGLEDGTCVTGEGFGAEGECSGELVFSTQMGGYMEALTDP
                     SYHGQILMFTFPTIGNYGVDRMNFQAPRVWALGCVAREICAVPEVRPAFADFFEENGL
                     LGIQGVDTRMLTVKTRVQGTLRAALLVGDEDAGRAVDMARRAKPISDAELIPAVSCTE
                     PYHIAGRGKRIAVIDLGIKKNMIISLRHRDADLHVFPYNSAPDQVMACRPDALFISNG
                     PGDPIHATDAIRCVKDLAGTLPIYGICMGNQICGLGLGGQTYKMKFGHRGTNQPVRYK
                     DGSIYITTQNHGFAVAPDTLPEGANVLYSNVNDGTLEGFEDSYLEITCVQFHPEAHGG
                     PRDTEIPFFDTMFRRLG"
     gene            1103..4270
                     /gene="carB"
                     /locus_tag="PKM87_08555"
     CDS             1103..4270
                     /gene="carB"
                     /locus_tag="PKM87_08555"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNQ30155.1"
                     /translation="MPKKAHIKKVLLIGSGPIQIGQAAEFDFSGSQACRALREEGVKV
                     VLVNSNPATIQTDPEMADEIYIEPIRAEIIEKIIEKERPDGILSGMGGQTGLNMTAEL
                     AERGALKGVEILGTPLEAIYQGEDREKFRALMEHIGEPVPRSMILNRLDQVEEALRTV
                     GLPAIIRPAYTLGGSGGGIAHTREELVRIIEIGLSRSRIHQVLVEESVVGWKEIEFEV
                     MRDAGDTCIIVCGMENVDPMGIHTGESVVVAPILTLRDDEFQMLRTAAIKIIRALDVQ
                     GGCNIQFAYQDGEYRVIEVNPRVSRSSALASKATGYPIARVAAKIAIGLRLDEITNSV
                     TGCTPASFEPAIDYIVVKVPRWPFDKFKNADRTLTTAMKSTGEVMAIGRTCEEAFKKA
                     LRSIDTDTDTHTNPNEIRMILSRPTDERFTTLFDAVRAGFSVEEIADLTRISPFFLEK
                     IRNIVDLEKKLKNTPTREDIRLAKQYGFTNTEIARESGSPIEQVLDMAGEPTYKIVDT
                     CAAEFPARTPYFYSTWDTECEIPKTDRGKILILGSGPIRIGQGIEFDYCTVHAVKALR
                     EENVEVHIVNNNPETVSTDFDTSDRLFFEPMLLEDVVNILKKDTYMGVMVQFGGQNAV
                     NLAVPLNDELKKINLPTRILGTTPDSMDIAEDRDRFSLLLGRLGIPSPPNSSAYSLEE
                     ARTRAAAIGYPILVRPSYVLGGRAMEIVHDEIELEGYMKEAVRVSRHHPVLIDSFLQN
                     AIELDVDAVCDGEEVLIGGIMEHIEQAGVHSGDSACVIPTQSLSPSILERVKDYTRKI
                     ALGLGVVGLVNIQLAVKDDVVYVLEANPRASRTVPFVSKATGIPLAKIAAKTMIGKKL
                     KEMGYREKAYSHVAVKEVLLPFNKLPGVDTVLGPEMKSTGEVMGIDYDFGRAYYKASI
                     SADNTIPVEGNIFISISKEQKDEVVPIAKKLKEIGLNLYGTSGTVDYLTEAGIPAQLV
                     RKVQEGSPNVIDLLRRGEIRMIINTPTDKQSRQDHYQIMRVAVDYGVPYITTIQAARA
                     AAMAIEAIKKERITIEPLSHYLGAM"
     gene            4452..5645
                     /locus_tag="PKM87_08560"
     CDS             4452..5645
                     /locus_tag="PKM87_08560"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNQ30156.1"
                     /translation="MGKGTIVMAYSGGLDTSICIPLLKERYGYDRVVTVAVHVGQRDE
                     EIRTATEKGLRLADTHYTIDSRERFVHEYIFPAIRANGSYEGYPMGTALARPLIAEEV
                     AKIAALEGSSAVAHGCTGKGNDQLRFDFVFRMAGLEVIAPIRELNLTRDWEIEYAREH
                     DIPVPVAKDRPWSVDENIWSRSIEGGRLEDPAYHPPEEIYAWSISPEKAPDTPEIVTI
                     DFIKGVPHRLNDVEYAGYDLIQRLNAIAGKHGIGRSNMIEDRILGLKAREIYEHPAAT
                     VLLAAHADLEHLTLTRQELAFKRIVDDKWSELAYMGLVHEPLFHDLNAFIARSQDRVS
                     GRVDVMLYKGCCRVLGRSSQEGIYSGDLVSFDTTTIDQCHAIGVSAYYGIQARLVEQG
                     RKKKD"
     gene            complement(5654..6058)
                     /locus_tag="PKM87_08565"
     CDS             complement(5654..6058)
                     /locus_tag="PKM87_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNQ30157.1"
                     /translation="MVKLTPDMKETFSKVNLFPVATASKNGVPNVAPIAFVIMMDDET
                     LWLADNFMNKTLANVKENPQAAIFVYDSYLKRCFQIKGSIAVKTSGPDYEKMKNMVHE
                     KKPGLPAKSLLVMKVTEVFECNPGANAGKKLL"
     gene            6249..7175
                     /locus_tag="PKM87_08570"
     CDS             6249..7175
                     /locus_tag="PKM87_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ30158.1"
                     /translation="MHRPSYLRLLETGELERRVDAAVALLSSCRVCPRECGIDRLNDE
                     AGYCRSGRLPVVSSYGPHFGEEPPLVGRHGSGTIFLAGCNMRCVFCQNYAISQEYCGR
                     EVSCGELAGMMMELCKQGCHNINFVSPTHFVPQILEAVRIAAGMGLDIPLVYNTGTYD
                     SVETLKILDGVFDIYMPDAKYGSDKLALSLSDAPGYVGIMKDAILEMQRQVGDLVVED
                     GIAVKGLLIRHLVLPENLAGSEEVFRFIAGNVSRGAYMNVMNQYRPCGRIVGDIHHPH
                     HRELMQGITSREYFEAITAARRYGLHRGFFSP"
     gene            complement(7188..7973)
                     /locus_tag="PKM87_08575"
     CDS             complement(7188..7973)
                     /locus_tag="PKM87_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ30159.1"
                     /translation="MNNRKIRVVVAGKGGVGKTTLTALLAHAFSRRGMPVLAVDGDPQ
                     ANLAATLGIPALHPITPLSEQKAYIQEKIGGVPGRGGFMVLNPDIADVADRFAVPAGE
                     RIRLLVMGGVRNAGSGCLCPEYTLISTILASTGCLPDEVILLDTPAGLEHFGRAVAQG
                     FNCALIVTDASYNARSVARDLSRLARQCGIRDVILVFNRMHSGSDLKEIAEEMEGLFL
                     DIRSMPHDPCLPRCEPSVVPVVEGECPVMKRVNSLAEFILSRD"
     gene            complement(7996..9954)
                     /gene="cooS"
                     /locus_tag="PKM87_08580"
     CDS             complement(7996..9954)
                     /gene="cooS"
                     /locus_tag="PKM87_08580"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HNQ30160.1"
                     /translation="MSEEQKPVLIREKMEVCELDRVRLSLMNPQLIEQKKEERTFDAA
                     AKPYLELTMKDGIETVWDRFEMQQPPCKHCETGLSCSRCAMGPCRIIPPNRIRGVCGA
                     DADLIVARNLLDTLATGAAAHSDHGREIVLTLYGIGKGTVKDYVVSDREKLIRIAGEF
                     GVDTKGGNEKKIAAALGSAMLEEYGSVKDSIQFAKRAPSSTQEIWAKAGISPRGIDRE
                     VVDSMHRVHMGVGADYQNILLQGLRTSLSDGWGGSMMATEISDVLFGTPVPNESLVNL
                     GVVKKDHVNISLHGHNPMLSEMVVKAAKDPGILALAKKVGAKGINLVGLCCTGNELLM
                     RKGIPNAGNHFNQELLIATGALEAMVVDYQCIFPSLPRTASCYHTHIISTSHKSKIPG
                     SYYFGFEPENAYATAKAIVKMAVENFQNRNPDRVFIPVEPVKVTTGFSVEAIRAALGG
                     TFKPLIDQIASGSIRGAVGIVGCNNPKVKHDYGHVTLGKELIRRDILCVETGCAAIAS
                     GKAGLLQPSASALAGPGLKSVCESLGIPPVLHMGSCVDCSRILHLAAELGNALNVGIH
                     KLPLAGAAPEWYSQKAVSIGSYFVASGVFTVLGVMPKIAGSPPVVSLLTEGLKGAVNA
                     SFAVEPDPLKAADLIADHIERKRTELGI"
     gene            complement(10154..10225)
                     /locus_tag="PKM87_08585"
     tRNA            complement(10154..10225)
                     /locus_tag="PKM87_08585"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10190..10192),aa:Arg,seq:gcg)
     gene            10555..10830
                     /locus_tag="PKM87_08590"
     CDS             10555..10830
                     /locus_tag="PKM87_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30161.1"
                     /translation="MILLRCRNCKGTGEVQNEEHRLCEALKSHTVKKYFHIPTEDEVG
                     SDQEVPPDACNGVPERIPCPVCEGAGTIAFDEDEWELKIVEEEDETG"
     gene            complement(10849..>11986)
                     /locus_tag="PKM87_08595"
     CDS             complement(10849..>11986)
                     /locus_tag="PKM87_08595"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HNQ30162.1"
                     /translation="RVYIVIDALHVALSRLASDDIAIVGDNEPTQLALLSAGIAVLVV
                     AEGAPVGERVLEAARRKGATILSTPLDAFSVGRMLHLSLPAGKVVATDVPVIRLEDSL
                     AYARKMVTDSKYRTACVVDENRALLGMISRNTFLDDVQKQVILLDHNEYAQAVEGVES
                     AEILEVIDHHRLGAITTLKPVRFQNEPVGSTSTIITRKFMESGTIPSPGTAGILLAGI
                     LSDTLILKMSTTTPEDVSAVEFLSGVTGIDAQQFGADLLQQGINLDSTPLHQLLSQDV
                     KRYTLFGREVIIAQVMTASRTYAEEHGKAIQAELENLRRGNSVDLYMVLFTDIIGNRS
                     DLFVSADHATLNVLGYGTQPVMLPGVMSRKKDFLPVFGGKLRDL"
BASE COUNT         2590 a         3506 c         3530 g         2360 t
ORIGIN      
        1 atcccgacac ttattaaaac gttccccgca caccatatat ggaaatcatg aaggcggttc
       61 tgggtctaga ggatggcacc tgcgtgacag gcgaagggtt cggtgccgag ggggaatgct
      121 ctggcgagct tgttttttcc acccagatgg gtggttacat ggaagccctc accgatccca
      181 gctatcacgg gcagatactg atgttcacct ttcccaccat cggcaattac ggtgtggaca
      241 ggatgaactt ccaggccccg agagtctggg ctctcgggtg cgtagcccgc gagatctgcg
      301 cggtccccga ggtaaggccg gcattcgcag actttttcga agagaacggc ctcctaggca
      361 tccagggagt ggacacccgg atgctcaccg tcaagacaag ggtgcagggg acgcttcggg
      421 ccgcgctcct ggtcggcgat gaagacgcag gccgcgcagt ggacatggcc aggagagcaa
      481 agccgatatc ggacgcagaa ctaattcccg ccgtctcatg cacagagccg taccacattg
      541 ccggaagagg aaagaggatc gcggtcatcg accttgggat aaaaaagaac atgattatca
      601 gcctgaggca ccgcgatgcg gacctccatg tcttccccta caacagcgcg cccgaccagg
      661 tcatggcctg ccgccccgac gctctcttca tcagcaacgg ccccggagac cccatccatg
      721 ccaccgatgc catccggtgc gtaaaagacc tcgcggggac ccttccgatt tatgggattt
      781 gcatggggaa ccagatctgc ggcctcgggc tcggaggcca gacctacaag atgaagttcg
      841 ggcaccgcgg caccaaccag cctgtccgtt acaaggacgg gagcatctac atcacgaccc
      901 agaaccacgg ctttgccgtc gcccccgaca cactcccgga gggggccaat gtcctctact
      961 cgaacgtgaa cgacggcacc ctggagggat tcgaggattc gtacctcgag atcacctgtg
     1021 tccagttcca tcccgaggcc catggcggcc cacgggacac ggagattcct ttctttgaca
     1081 ctatgttcag gaggctgggc tgatgccaaa gaaggcgcat ataaaaaagg tcctcctgat
     1141 cggctccggg ccaatccaga tcgggcaggc cgcggagttc gacttctcgg gctcgcaggc
     1201 atgcagggcg cttcgcgagg agggcgtcaa ggtggtgctg gtgaactcga accccgccac
     1261 catccagaca gaccccgaga tggcagacga gatctacatc gagcccatac gcgcagagat
     1321 catcgagaag atcattgaga aggagaggcc cgacggaatc ctctcaggga tgggagggca
     1381 gaccggcctg aacatgaccg ccgagcttgc agagcggggg gcgctgaaag gagtggagat
     1441 cctcgggact cctctcgagg ccatctacca gggagaggac cgcgagaagt tccgtgccct
     1501 gatggagcat ataggagagc cagtcccgag gagcatgatc ctcaaccgcc tggaccaggt
     1561 agaggaggcg ctcaggacgg tcggcctccc tgccatcatc cggccggcgt atactctcgg
     1621 cggatcgggt gggggtattg cccatacccg ggaagaactg gtccgaatca tcgagatcgg
     1681 gctctccagg tcccgcatcc accaggtgct ggtggaggag tccgtggttg gctggaagga
     1741 gatcgagttc gaggtgatgc gggacgcggg agacacctgc attatcgtct gtggcatgga
     1801 gaatgttgac ccgatgggca tccacaccgg cgagagcgtg gtggtagccc cgatcctcac
     1861 gcttcgggac gacgagttcc agatgctccg gacggcggcc atcaagatca tcagggcgct
     1921 cgacgtgcag gggggctgca acatccagtt tgcataccag gatggcgagt accgggtcat
     1981 cgaggtgaac ccacgcgtct cgcggtcatc cgcactcgct tcgaaggcca cgggataccc
     2041 gattgcccgg gtggctgcaa agatcgcgat tggcctgcgc ctggacgaga tcaccaacag
     2101 cgtcacgggc tgcacccctg cctcgttcga gcctgcaatc gactacattg tcgtgaaggt
     2161 cccccggtgg ccgttcgaca agttcaagaa tgccgaccgg accctgacga ctgcgatgaa
     2221 gagcacgggt gaggtgatgg ccatagggcg gacgtgcgag gaagcgttca agaaggcgct
     2281 gcgttccatc gatacggaca ccgacacgca taccaatccg aacgagatcc gcatgatcct
     2341 ctcccgcccc accgacgaga ggttcaccac gctctttgat gctgtccgcg ccgggttctc
     2401 cgtagaggag atcgcggatc tcacccgcat ctcccccttc ttcctcgaaa agatccgaaa
     2461 cattgtggac ttggagaaga agctcaaaaa cactcccacc agggaagaca tcaggcttgc
     2521 aaagcagtac gggttcacca ataccgagat tgcgagggaa tcgggctctc ccatcgaaca
     2581 ggtcttggat atggccgggg agcccaccta caagatcgtg gacacctgtg ccgccgagtt
     2641 ccctgcccgc acaccgtact tctactccac ctgggacacc gagtgcgaga tcccaaagac
     2701 ggatcgcgga aagatactca tccttgggtc agggccgata cggatcggac aggggataga
     2761 gttcgactac tgcacggtcc atgcggtgaa ggcgctgcgg gaagagaacg tggaagtcca
     2821 catcgtcaac aacaaccccg agacggtctc caccgacttc gacacctcag atcgtctctt
     2881 ctttgagccc atgctcctcg aggatgtcgt caacatcctg aaaaaagata cctatatggg
     2941 agtgatggtc cagttcgggg gccagaacgc ggtcaacctg gccgtcccgc tcaacgatga
     3001 gctgaaaaag atcaacctcc ccaccaggat actcgggaca acccctgatt cgatggacat
     3061 cgccgaagac cgggaccgct tcagtctcct cctcgggagg cttggaatcc ccagtccgcc
     3121 gaacagctcg gcatattcgc tcgaagaagc gaggacaagg gcggccgcaa tcggataccc
     3181 gatcctcgtc cggccctcct acgtgctcgg cggccgcgca atggagatcg tccacgacga
     3241 gatcgagctc gaggggtaca tgaaggaggc ggtgcgggtc agcaggcacc acccggtgct
     3301 gatcgactcg ttcctgcaga atgccatcga acttgacgtg gacgcggtct gcgacgggga
     3361 agaggtgctg atcggcggga tcatggagca catcgagcag gcaggggtgc acagcggtga
     3421 ctcggcctgt gtcatcccca cacagtcgct ctcgccctcg atacttgagc gggtgaagga
     3481 ctacacgaga aagattgcac tcggcctcgg agtagtggga ctcgtgaaca tccagctcgc
     3541 ggtcaaggat gacgtggtct acgtactcga ggcaaaccca agggccagcc ggacagtccc
     3601 gttcgtctcg aaggcaaccg gtatcccgct cgcgaagatt gctgcaaaga ccatgatcgg
     3661 aaagaaactg aaagaaatgg gataccgcga gaaagcatac tcccacgtgg cggtcaagga
     3721 ggtgctcctt cccttcaaca agctccccgg cgtggacacg gtgctcgggc ccgagatgaa
     3781 gagcaccggt gaagtgatgg gcatcgacta tgacttcggg cgggcctact acaaggccag
     3841 catctctgca gacaacacca tccctgtcga ggggaacatc ttcatctcca tctccaagga
     3901 acagaaggac gaggtggtcc caatcgcaaa gaagctgaag gagatcggcc tgaacctgta
     3961 cgggacctcc gggactgtcg attaccttac cgaggcaggc atccccgcgc aactggtgag
     4021 aaaagtccag gaagggtcgc cgaacgtcat cgatctcctc aggagagggg agatacggat
     4081 gatcatcaac acacccaccg ataagcagtc aaggcaggac cactaccaga tcatgcgggt
     4141 cgccgttgac tacggcgtcc cctatatcac caccatccag gccgcccggg cggctgcgat
     4201 ggcaatcgag gccatcaaga aggagaggat caccatcgag ccgctctccc attaccttgg
     4261 ggcaatgtaa gcgtcctcat gcaggaacac tcctccattt tttcccggtt cccggtaatc
     4321 accaccagtc cctgcagaag ggaacgaccc cggcccggcc agtcctctcc ggaataccct
     4381 taatacatcg gcaggggcaa tgaataggta tcacctgtga gggtgtggta ctctcacgca
     4441 ggagtgagcg aatgggaaaa ggaaccattg tcatggcata ttccggggga ctcgacacct
     4501 ccatctgcat ccccctgttg aaggaacggt acgggtacga cagggtggtc accgtcgcgg
     4561 tgcacgtggg ccagcgggac gaggagatcc ggacggccac cgagaaaggg ctgaggcttg
     4621 cagatacgca ctacaccatc gattcccgcg agaggttcgt ccacgagtat atcttccctg
     4681 ccataagggc caatggttcc tacgagggat accccatggg gaccgcgctt gcccgaccgc
     4741 tcatcgccga agaggtcgcg aagatcgccg cattggaggg atcttctgcg gtcgcccacg
     4801 ggtgcaccgg caagggaaac gaccagctcc ggttcgactt tgtcttccgc atggcgggac
     4861 tcgaggtcat cgctcccatc cgcgagctga acctgacccg cgactgggag atagagtatg
     4921 caagggagca cgatattccc gtcccggtcg caaaggaccg gccctggagc gtggacgaga
     4981 acatctggag caggagtatc gaggggggga ggctcgagga ccccgcgtac cacccgcccg
     5041 aggagatcta cgcctggagc atctctcccg agaaggcacc cgatactccc gagatcgtga
     5101 cgatcgattt catcaagggt gtcccccacc gcctgaacga cgtggaatat gcaggctacg
     5161 acctgataca gcgtttgaac gcgattgccg gaaagcacgg cataggccgg agcaacatga
     5221 tcgaggaccg catcctcggc ctgaaagcaa gggagatcta cgagcacccg gcggcaacag
     5281 tcctcctcgc ggcgcatgcc gacctggagc acctcaccct cacacggcag gagctggcct
     5341 tcaagagaat agtggacgac aagtggtccg agctcgccta catggggctc gtgcacgaac
     5401 cgctctttca cgacctgaac gccttcattg caaggtcaca ggaccgggta tcggggaggg
     5461 tggacgtcat gttgtacaag gggtgctgca gggtgcttgg ccgctcgtcc caggagggca
     5521 tttactcggg cgacctcgtc tcgttcgata ccaccaccat cgaccagtgc cacgccatcg
     5581 gggtctcggc atactacggg atccaggcac ggctcgtgga gcagggacga aaaaagaagg
     5641 attaatattt tttttagagc agtttcttcc cggcgttcgc tccggggttg cactcgaaga
     5701 cctcggtgac cttcatcacc aggagtgatt ttgcaggaag ccccggtttc ttctcgtgga
     5761 ccatattttt catcttctcg taatccgggc ccgatgtctt cactgcgatt gatcccttga
     5821 tctggaagca cctcttcagg taggaatcgt acacaaaaat tgctgcctgt gggttctctt
     5881 tcacgttggc aagggtttta ttcatgaaat tatccgccag ccagagcgtc tcatcgtcca
     5941 tcatgatcac gaatgcgatg ggggcaacat tcgggacacc gtttttggat gcggttgcca
     6001 cggggaagag attcaccttg gaaaaggtct ctttcatgtc gggtgtgagt tttaccattg
     6061 tcagattcac ctctttattg tgttgcacta cagcttggaa tagccgtcaa tactccaatg
     6121 tgcctcactc cccatattct ttttggcaaa ttcctgcggc aatggaggat aatgcagcga
     6181 atggctgata tattgtgagg gacacgaact ggtaacacac gccgtgtggt gacaggtggg
     6241 gaactcctat gcacaggccc tcgtatctgc gacttctgga gacgggcgaa ctcgaacggc
     6301 gggtggacgc tgctgttgcc ctgctctcgt cctgcagagt ctgcccccgc gaatgcggca
     6361 tcgaccggct caatgatgaa gcggggtact gcaggagcgg gcgcctcccc gtagtctcct
     6421 cgtatgggcc ccattttgga gaggagcccc ctctcgtggg gaggcacggt tccggcacta
     6481 tcttcctggc aggatgcaac atgcggtgcg tattctgcca gaactacgcc atcagccagg
     6541 aatactgcgg gagagaggtc agctgcgggg aacttgcagg gatgatgatg gaactctgca
     6601 agcaggggtg ccacaacatc aacttcgtct ccccgaccca cttcgtgccg cagatccttg
     6661 aagcggtgcg tatcgcggcc gggatggggc tggatattcc ccttgtatac aacacgggaa
     6721 cgtacgactc tgttgagacg ctgaagattc ttgacggagt attcgacatc tacatgcccg
     6781 acgccaaata cgggagtgac aagttggcac tctcgctctc tgatgcgccg ggatatgtcg
     6841 ggatcatgaa ggatgccatc ctcgagatgc agcgccaggt gggcgacctc gtcgtggagg
     6901 acgggatcgc ggtaaaagga ctcctcatcc gccacctcgt cctccccgaa aaccttgcgg
     6961 gaagcgagga ggtgttccgg ttcattgccg ggaatgtctc gaggggcgca tacatgaacg
     7021 tgatgaacca gtatcgcccc tgcgggcgga ttgtggggga tatacaccat cctcaccacc
     7081 gcgaactcat gcaggggatc acctcgaggg aatacttcga agcaattacg gcggcacgga
     7141 gatacggtct tcacaggggc tttttttctc cgtgacgcaa attcaggtca gtcgcgtgaa
     7201 aggatgaact ccgccaggga attgaccctc ttcatgaccg ggcattctcc ctccacaaca
     7261 ggaaccaccg atggttcgca ccgtggtaag cagggatcat gcggcatgga cctgatatca
     7321 aggaagagtc cttccatctc ctccgcgatc tctttcaaat ccgatccgga gtgcatgcga
     7381 ttgaagacaa ggatcacgtc cctgatgccg cactggcggg ccagcctgga gagatcccga
     7441 gcgaccgacc gtgcgttgta ggacgcatcg gtcacgatga gtgcacaatt gaacccctgg
     7501 gcgaccgccc gcccgaaatg ctcgagcccc gcgggggtgt cgaggaggat cacctcgtcg
     7561 gggaggcacc ctgtgctggc aaggatggtg gagatgagcg tgtactcagg gcagagacaa
     7621 ccggaacccg catttcggac gcctcccatc accagaaggc ggatgcgctc accagcaggg
     7681 accgcgaacc ggtcggcaac atccgcaata tcggggttga ggaccatgaa ccccccgcgc
     7741 ccggggactc ccccgatctt ctcctggatg taagccttct gctccgagag gggcgtgatc
     7801 ggatgcaagg caggtatccc gagcgttgcg gcgaggtttg cctgggggtc cccgtcgact
     7861 gccaggacag gcatcccccg ccgcgaaaac gcgtgcgcca ggagggcggt gagggtggtc
     7921 ttgcccaccc cgcccttccc ggcgacgact acccgaatct tcctgttgtt catgatggga
     7981 aaaaaggaaa aatgttcaga tccccagttc agtccgcttc cgctctatgt ggtctgcaat
     8041 gaggtccgcg gctttcaggg gatctggctc gactgcgaac gaggcgttca ccgccccttt
     8101 caacccttcg gtgaggaggg agaccactgg cgggctcccc gcgatcttcg gcatgacgcc
     8161 aagcaccgtg aacactcccg atgctacgaa gtaggagccg attgaaaccg ctttctggga
     8221 gtaccactcg ggtgctgcgc cggcgagggg gagcttgtgg atcccgacat tcagggcgtt
     8281 cccaagctcg gccgcgaggt gcaggatgcg ggagcaatct acgcatgagc ccatgtgaag
     8341 aaccgggggt atcccgagcg attcgcagac tgatttgaga cctggaccag cgagagcaga
     8401 ggcagacggc tggagcagcc ccgccttccc ggacgcgatt gccgcacacc ccgtctcaac
     8461 gcagaggata tcccgcctga tcagctcctt gccgagcgtg acatggccgt agtcatgctt
     8521 caccttgggg ttgttgcacc cgactatccc caccgcgccc cggatgctgc cgctcgcgat
     8581 ctggtcgatc agcggcttga acgtgccgcc gagcgctgcc ctgatggcct cgaccgagaa
     8641 gccggtggtc actttcacgg gctccacagg gatgaagacc cgatcagggt tcctgttctg
     8701 gaaattctca acggccatct tcactatggc ttttgcggtg gcatacgcgt tctccggttc
     8761 gaacccgaag tagtaggaac ccggtatctt cgacttgtgg cttgtcgaga tgatgtgcgt
     8821 gtgatagcag ctcgctgtac ggggaagcga ggggaagatg cactggtagt ccaccaccat
     8881 cgcctcaagc gcgccggtcg cgatcagcag ctcctggttg aagtgatttc ccgcgttcgg
     8941 gatgcccttt ctcatcagga gctcgttccc cgtgcagcac aggccgacaa ggttgatccc
     9001 ttttgcccca accttcttcg caagcgccag gataccggga tctttcgctg ccttcaccac
     9061 catctcggag agcatggggt tgtggccgtg gagcgagatg ttcacatggt cctttttgac
     9121 gaccccgagg ttgacgagcg attcgttcgg gacaggggtg ccgaacagga cgtccgagat
     9181 ctcggtggcc atcatcgagc cgccccagcc atcagaaagc gatgtccgca ggccctgcag
     9241 gaggatgttc tggtaatcgg cccccacccc catgtggacg cggtgcatcg agtccaccac
     9301 ctcccggtcg atgccgcgtg gggagatgcc cgcttttgcc cagatctcct gcgtagacga
     9361 gggtgcccgc ttcgcgaact ggattgagtc cttcacggac ccgtattcct cgagcattgc
     9421 agatcccagg gcggcggcga tcttcttctc gttcccgccc tttgtgtcca ccccgaactc
     9481 accggcaatc ctgatcagtt tctcccggtc cgagacgaca tagtccttca cagtcccctt
     9541 cccgatgcca tagagggtca gcacgatctc gcggccgtga tcggagtgtg ccgcggcccc
     9601 tgttgcgagc gtatcaagga ggttccttgc cacgataagg tctgcgtctg ccccgcatac
     9661 tccccttatg cggttcggag ggatgatccg gcatggcccc atcgcgcagc gtgaacagga
     9721 gaggcctgtt tcgcaatgct tgcatggcgg ctgttgcatc tcgaagcggt cccagaccgt
     9781 ctcgattccg tctttcatcg tcagctcgag gtacggtttt gccgctgcat caaacgtccg
     9841 ttcctccttc ttctgctcga tcagctgggg gttcatcagg gaaaggcgga cacggtcgag
     9901 ctcgcatacc tccatctttt ccctgatcaa cacgggcttc tgctcttcgc tcatagtcag
     9961 gtcaccacat tgatatggca gaagtcacat ggaaggaata tttgtaattt ctggtagagg
    10021 caacgggaga aggcacatcc tccgtttatc aggcgattgt tcagggcttc ggggtcaacc
    10081 atgagggcat gtcatgggaa ggaattgaga gaatgaaaag gggaggttgc gttcctcccc
    10141 ctaaaaaatg atacgccccg gccgggactt gaacccgggt caaaagctcc gcaggcttct
    10201 aggatatcca ctaccctacc gggacaacct ggctataact atctcgcctg tatgcataaa
    10261 taattgacct ttggatattt ttcctttagt tggtcttgaa tgggactatc atgagtggat
    10321 atccagatag caaggaggtc tatcacaatt tttctgattt tcgcacacat tgaattcact
    10381 cggtttctca ttcctcctcc cttttggtgt tcaggattct ggcctcatac ccaggcacag
    10441 ggcaggtgtg agaacattaa gggttcattc agtttcattt tttttctccc attcgtatct
    10501 taattaataa aatcagaata ttgaatttgg aataaggaat acctgaacag tgagatgata
    10561 ctcctccgct gccgcaactg caagggaaca ggcgaggtcc agaacgagga gcacaggctc
    10621 tgcgaagcgc tgaaatcgca caccgtaaag aaatacttcc acatccccac cgaggacgag
    10681 gtgggctccg atcaggaagt ccctcccgat gcctgcaacg gggtacctga aaggatcccc
    10741 tgcccggtgt gcgaaggtgc cgggaccatc gcattcgatg aagacgaatg ggagctgaag
    10801 attgtcgaag aagaagacga gaccggatga aaatacccag tcacactttt ataagtcccg
    10861 cagcttgccc ccgaataccg ggagaaagtc cttctttctc gacatgacgc ccggaagcat
    10921 gaccggctgc gtcccatacc caagtacgtt cagtgtcgcg tgatccgcgg acacaaagag
    10981 gtcgcttcgg ttcccgatga tgtcggtgaa caataccatg tacaggtcga cggagtttcc
    11041 cctccggagg ttctccagtt ccgcctggat tgcctttccg tgttcctccg catacgtccg
    11101 cgatgccgtc atcacctggg cgatgatgac ctcccgccca aagagggtat atctcttgac
    11161 atcctgcgac aggagctgat gaagaggcgt gctgtcgagg tttatccctt gctggaggag
    11221 gtcggctcca aactgctgcg catcaattcc tgtcaccccg gagaggaatt ccaccgcgga
    11281 cacatcctcg ggggtggtgg tcgacatttt gaggatgagt gtatcggaga gtatccctgc
    11341 aaggagaatt cccgccgtac ctggtgaggg aattgttccc gattccatga acttgcgggt
    11401 gatgatggtc gaggtcgacc ccaccggctc gttctggaac cggacaggct tgagcgtagt
    11461 gatggccccg agcctgtggt ggtcgatcac ctcgaggatc tccgcggact ccactccctc
    11521 gaccgcctgc gcatactcgt tgtgatcgag gaggatgacc tgcttctgga catcgtcaag
    11581 gaacgtgttc ctcgagatca tcccaagcag ggcccggttc tcgtccacca cgcaggcagt
    11641 gcggtacttg gagtcggtca ccatcttcct ggcataggcg agggaatctt cgagccttat
    11701 cacggggacg tcggttgcca ccactttccc ggcgggcagc gagaggtgga gcatcctgcc
    11761 gactgaaaac gcatcgagag gagtggagag gattgtggcc ccctttctcc ttgcggcttc
    11821 gagcacacgc tcccccacag gcgcaccctc cgcgacaaca agtaccgcaa tgcctgcaga
    11881 gagaagtgca agctgggtcg gctcgttgtc ccccacaatc gcgatatcat ctgaagccag
    11941 ccgggagagg gccacgtgga gggcgtcgat gacgatgtag accctg
//
LOCUS       DAOQYR010000071        11343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_6171_length_11343_cov_9.441442, whole genome shotgun sequence.
ACCESSION   DAOQYR010000071 DAOQYR010000000
VERSION     DAOQYR010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 11343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11343
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6171_length_11343_cov_9.441442"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="PKM87_08600"
     CDS             complement(<1..821)
                     /locus_tag="PKM87_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 14 protein"
                     /protein_id="HNQ30163.1"
                     /translation="MCARFLDRFFKPRPHIVESPPPPSIAHGPGVYVPEFKIKPYFIV
                     ASVEMGNTTTKCILTGVNLETGMSYVIHKTVKMSRDVRKPKPGEDVFGETLDGTQLTR
                     ESVTELVRDTLVECHRDVHLDIQKDLDFVVRSTGVVAAMDSPDQVGEFVLALANGCLI
                     AGVPPRKMTPPMSKANQAQKLQPFSYADKVVFIGAVAGVIPPVGSTGVEMVANEMEGE
                     LAMAGIKEGAKWTPVDFRNPCISIDFGTTLDGRITSDVAPDEKNPFAKTIGNFCGL"
     gene            complement(946..1956)
                     /locus_tag="PKM87_08605"
     CDS             complement(946..1956)
                     /locus_tag="PKM87_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30164.1"
                     /translation="MTRSSVLLLVLFVLLLPCAVHALVTETNDQVLLNQPIDDDVIAA
                     GGKVAVNAPVRSLIAAGGEIEINAPVEGDVIAAGGTITLNAPVTGKVLLAGGLVTLNS
                     SVGRNVLIHSGEVNITSAATVGADALVSGGRVFHHGDVKGNLTVSSEDFTSAGTSRHL
                     SYTRDSASLGERMLGLVSFALILFSLGMAILGLLAIRFAPVPYMAVEARVRSRPLVNV
                     LAGLGGILGAIVLGVLLAITVVGLPLAICIVVVLVAGLLFATLFVSSSLGYVIGRRLR
                     WKTKAWQQFVIGFVILQIAFRIPYIGLVLLAISVCLGFGAFLFEFSGSGFIPENNGRT
                     RM"
     gene            2155..2358
                     /locus_tag="PKM87_08610"
     CDS             2155..2358
                     /locus_tag="PKM87_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30165.1"
                     /translation="MDMRDPRGWVFYYLICFVALLWVGFLTAQGVMIFVSLFLTLIVL
                     GFSLILCMMLVLELKNEKETEMQ"
     gene            2447..2692
                     /locus_tag="PKM87_08615"
     CDS             2447..2692
                     /locus_tag="PKM87_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30166.1"
                     /translation="MRASSFTILLIVLLMIIIILALIVIIGLTSMIGAGSTAGEETGP
                     IIQFVSLAQEQVLAFFDCLQQWFNYLIIQIQKLSEAL"
     gene            2804..3082
                     /locus_tag="PKM87_08620"
     CDS             2804..3082
                     /locus_tag="PKM87_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNQ30167.1"
                     /translation="MRMEHVKGSKRGELILYALSTCGWCAKTRKLLDDLGVEYSYVYV
                     DRLPHEEMESAIREVEKHNPSGSFPTLVIDGSRAIVGFREADIREALK"
     gene            3079..3585
                     /locus_tag="PKM87_08625"
     CDS             3079..3585
                     /locus_tag="PKM87_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HNQ30168.1"
                     /translation="MTGEGIPEQTIRKKADKIRSDASAGGYLLNPDQSFVRELVAGIC
                     VNEQRYGYPACPCRLASGSREEDLDIICPCDYRDADLNEFGACYCALYVSAGIASGEA
                     QVTCVPERRPSRKERRKESRSAPVFAGELPEPVWRCRVCGYLCAREGPPLVCPICKAS
                     QDRFVRFI"
     gene            3698..4045
                     /locus_tag="PKM87_08630"
     CDS             3698..4045
                     /locus_tag="PKM87_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30169.1"
                     /translation="MADTISIEVVGLKDSTCSPFPCDMTRSCGLYDCHPTGKLVPAFE
                     ALKKELVSQYGESVRMTLVLIDEDMPDHVREIIATHYPPIPFILMNGALIPMGRISLP
                     LIQKEIEKILPIR"
     gene            complement(4038..4619)
                     /locus_tag="PKM87_08635"
     CDS             complement(4038..4619)
                     /locus_tag="PKM87_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNQ30170.1"
                     /translation="MIDELLEGNKKFREGFFKENRAHHQELTKGQSPKVLWIGCSDSR
                     LQTGHITQTLPGTLFMHRNIGNIVPLQDWNFATVLEYAIRHLKVKDIVVCGHSDCGAI
                     KALDKETDDVYIPLWLNYAIEAKHRVDDTLPAPKTPEEVKKRSKMIEKENIRLQIEHL
                     MQYPIVKMAIKEKKVQVHGLYYDLDNGELSRIT"
     gene            4771..5553
                     /locus_tag="PKM87_08640"
     CDS             4771..5553
                     /locus_tag="PKM87_08640"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HNQ30171.1"
                     /translation="MKALPGMIRKAGAASSRFWEVDYCRGVAVVMMILFHAAFDMWYL
                     GVLDIQVQSGPWKILAVCTASLFLLIVGLSLSISSARARKTLHRREFILKYLRRGVGI
                     MGLGLLLTLVTMAMVPTEPILFGILHLIGLSVMLAPLYVRYTWANLVAGLAVIAVGWG
                     IKGLYGPVWLIWLGVHPQGFASLDYTPIFPWLGVVLLGVWLGHLLYPGGMRRAPLPVP
                     DMPAREIVCFAGRHSLMIYLVHQPILLLVISILWSLWGIPIV"
     gene            complement(5565..7430)
                     /locus_tag="PKM87_08645"
     CDS             complement(5565..7430)
                     /locus_tag="PKM87_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ30172.1"
                     /translation="MNVIPAMTQKSTSELTISSRTQVFLIVLVTILVSIGYVAGTMLG
                     LSAITPLLFYLPIILAAYWFPRRGVLFAVGVGIAEVFFVYLYQYPSLPDITFAITTAS
                     FYVLVAIAVVISSLSSGLRDREARYRAIFSGSEASIFLVQNGDSELRIEEVNPGGGTL
                     IGRHPDDLKGESLIVFWRDDLARDSLLQSMKDDGSVSQMESVMTRENGTTVPVLISGS
                     RLPGRMMVLTVIDISARKAHEEEIEARNTQLSTINRVIAEAAAATGVEEMGRGVLANL
                     TRYLGCEYGGISLYEEGSSRLVAKVHHGDDSLYQNLMESGNESVAALKEAVSQGKFLV
                     WKEESGTQCDTPASGIVVPLQSGEEALGVMYFLSCSSRTCSKDQQQTLESLSREIATT
                     VTRLLLSQRITETNQQANLYLDILMHDINNANLASLWYGDLLLEMLSGESKEIATKMI
                     EGIKKSREIIRNLETIRKIQVRKNDLRAVTLDTAIQKEVRLFPEVRIECEETGAMVRA
                     DDLIGEIFNNLIGNSIKFGGSAVNICISVDSTNPAFVVVEVADNGPGIPDDLKSVIFR
                     RFSQSDRQNAGKGLGLYIVKTLLERYGGTISVNDRVAGDHSQGVVFRMTFQKAQP"
     gene            complement(7515..8882)
                     /locus_tag="PKM87_08650"
     CDS             complement(7515..8882)
                     /locus_tag="PKM87_08650"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ30173.1"
                     /translation="MEFQVGMSITGEGMISVLFIDDEPALLEITRLFLERSGEIRVET
                     CRSALEALEILKTRSFDAIISDYEMPMMDGIVLLKILRAEGDNTPFIIFTGKGREHVV
                     VDALNNRADFYLSKGQDPKAQFAELDRMIRQAINRRIAEESLRLADRRMVDIINHLPD
                     ATFATDREGVVVSWNKAMEDLTGVPAREMIGKGNHEYAIPFYDSPRPLLVDLINKPED
                     EIRAHSYQNIRRDGNSIIAENPTGLRNGKPAVFWSRATLITDRKGTVIGAIESIRDIT
                     EVRSAEGKQKDQAAREKESGMFDRIFGKSTASWYKKGVDLYYNHGRFQDAVSCFDRVI
                     EMDPTHVNAWTEKGICLKELGRYDEALQCFNRALELNGKLPATYYAKGETLEKLGKET
                     GNFSLFEQAIACFDLVLQKEPDNVNAWNYRGVCLKELGRFEEARRSFDKAQTILRLHP
                     QKGLR"
     gene            complement(8998..10668)
                     /gene="ade"
                     /locus_tag="PKM87_08655"
     CDS             complement(8998..10668)
                     /gene="ade"
                     /locus_tag="PKM87_08655"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HNQ30174.1"
                     /translation="MEDFPSPAMMRQALGKEPADALFKNACVYDPFTCGWIETDIAVA
                     SGKIVGLGSLYKARNTLNLKGRRVVPGLIDAHVHVESSLLVPSEYARVVAMHGTTSVV
                     ADPHEIANVCGKAGIEYMLREREGLPVDIFIMLPSCVPATPLDRGGAELTAEDLSEFI
                     GRDGVLGLGEVMNVPGVLERDEGVFAKLALCSIRDGHAPLLSGESLNAYILAGLQSDH
                     ECTGKEEAAEKLSRGMFIFAREGSTEKNLRELIPLASPCTASRIAFATDDRHADSLVE
                     EGHIDDCIRKSLEYGLELEIVLRMATLSAADRFGLHDRGALAPGRNADFCILLEDEPF
                     SVLKTFKSGKILDLSVHKKPRIIPSPMRARAPTHEDLAIKGEGTARVIGIVPHQILTE
                     HLAFELEGAEIPDLDRDILKVVVCNRYREGRCGLGLVHGFGLKKGAIAGSIAHDAHNL
                     VAVGADDQAIREALGGIIRNGGGMAVAGPDSLELLPLDCGGLMSSLPAEEVAGLLKTL
                     NGKAVDLGAIPGAFMYLSFLSLTVIPHLRVTDRGMFDADVFRDVPLFR"
     gene            complement(10858..11112)
                     /locus_tag="PKM87_08660"
     CDS             complement(10858..11112)
                     /locus_tag="PKM87_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30175.1"
                     /translation="MPPNIYEKVNKLVYEEKKFSTVSDCITQALIYFVDNQNDVGQFK
                     ENLHDYLASEEGKDFMKKIMKDLLVDVLTTQQKIAAEERR"
BASE COUNT         2495 a         3057 c         3032 g         2759 t
ORIGIN      
        1 agcccgcaga agttaccgat agtcttggca aacgggttct tctcgtcggg ggccacgtcg
       61 ctcgtgatgc ggccgtcgag tgtagtccca aaatcgatcg agatgcaggg gttccggaaa
      121 tcgacagggg tccactttgc gccttccttg atgcctgcca ttgcgagctc accctccatc
      181 tcgtttgcga ccatctccac gcccgtgctt ccaaccgggg ggataactcc cgcgactgcc
      241 ccgatgaaga ccaccttgtc tgcgtaactg aatggctgga gtttctgggc ctggttggcc
      301 ttcgacatgg gaggggtcat cttcctggga gggacccctg caatcaggca gccgtttgcg
      361 agtgccagca caaactctcc aacctggtcg ggagagtcca tcgccgccac cacgcctgtg
      421 ctcctcacga cgaagtcaag gtccttctgg atatcgaggt gtacatccct gtggcattcg
      481 accagggtgt cccgcacgag ctccgtaacg gactcccgcg tcagctgtgt cccatccaga
      541 gtctccccga atacatcctc gccaggcttt ggcttacgga catccctgct catcttcacc
      601 gtcttgtgga tgacgtaact catccccgtc tccaggttca caccggtgag gatacatttt
      661 gtggtggtgt ttcccatttc cacggatgca acgatgaaat aaggttttat cttgaattca
      721 gggacataca cgccaggacc gtgcgcgata gagggtggag gcgggctctc cacgatatgt
      781 ggcctgggct tgaaaaagcg gtccaaaaaa cgcgcacaca tagtatcgaa tgggatgctt
      841 gaccggcata tatctttcga tttacacctt ttcccttctg tttccggtaa tttccccgca
      901 tccagcagcc tttttttatg tggggagggg tgctccctac acccgttaca ttcttgttct
      961 cccattgttc tcaggaatga agccggaacc tgaaaattca aacaggaatg ccccaaagcc
     1021 gagacagacg gatatggcaa gtaataccag gccaatgtac gggatcctga aggcgatctg
     1081 gaggatcaca aagcctatca cgaactgctg ccatgccttc gttttccatc tgagacgtct
     1141 cccgatcaca tacccaagtg acgaagatac aaagagcgtg gcaaaaagaa ggcctgctac
     1201 cagtaccacg acgatacata tcgcgagggg aagtccgact acggtgatgg caaggaggac
     1261 tccaaggacg atcgcgccca gtatgccacc cagtcccgca agcacattta ccagggggcg
     1321 ggacctgacc cgtgcttcga ctgccatgta gggtacaggt gcgaacctga ttgccaggag
     1381 tccaaggatg gccataccga gtgagaaaag gatcagtgca aaagagacca gcccgagcat
     1441 acgttcaccg agcgatgcgc tgtccctcgt gtacgagagg tggcgggacg tcccggcaga
     1501 agtgaaatcc tcgctcgaca ccgtaaggtt acctttcacg tcaccatgat ggaacaccct
     1561 tccacccgaa accagcgcat cggcgcctac tgttgcggcg ctggtgatgt tgacctcacc
     1621 ggagtggata agcacgttcc tgcccacgct gctgttcagc gtgaccaacc ctccggcgag
     1681 gaggactttt cctgtcaccg gggcattgag agtgatcgtc ccaccagcgg cgatcacatc
     1741 gccctcaacc ggtgcattga tctctatctc accaccggct gcgatgagac tccggacagg
     1801 tgcattcacg gccacctttc cgcctgcagc gatgacgtcg tcgtctatcg gctggttcag
     1861 caggacctgg tcattggtct ctgtgacgag ggcatggaca gcacagggga gcaggaggac
     1921 aaaaagtaca agcaggagga ctgatgatct ggtcatgcaa agagcatcag cgggttaaat
     1981 gagaaaagta tcggtaaaaa cctctataat ttcccttcgg gacccgtgca tgcaaaatga
     2041 aaatggagca gccattttca gacgaaaaag gtcggttaag gattattctc ccccccatcc
     2101 ctcatcagac agtaaatgaa aatatttaaa tttggttgcg atgaatggcg atacatggat
     2161 atgcgcgacc cccgggggtg ggtcttttat tatctgatct gttttgtggc actcctctgg
     2221 gtgggatttc tcaccgctca gggggttatg atctttgtga gtctcttcct caccctcatc
     2281 gtgctcgggt tctctctgat cctctgcatg atgctggtac tggagttgaa gaacgaaaaa
     2341 gagacagaaa tgcaatgaaa ggaaggattt ttttgcgcat ttacccgtca atgggaaatg
     2401 tcattcttta aatagtctga aacactacca tcccatggag gggagcatga gggcaagttc
     2461 ctttaccatt ctgctgattg tgctcctgat gatcatcatc atacttgcgc ttattgtgat
     2521 cataggtctc acctccatga tcggcgcagg gagcactgcg ggagaagaaa cagggcctat
     2581 catccagttc gtctcccttg cccaggagca ggtgctggca ttcttcgact gcctccagca
     2641 gtggttcaac tatcttatta tccagatcca aaagctctcc gaagcactct gacaagaagt
     2701 attcaagtgt gcgcctggat ccgccgattc cccggctgat agggttatat tccccctaaa
     2761 accatcatac cagtaaggag aaagattcct tgagtgtgta aaaatgcgga tggaacatgt
     2821 gaagggcagc aagagaggag aactcattct ctatgcactc tcaacctgcg gctggtgcgc
     2881 gaagacccgg aaactcctcg atgacctcgg ggtcgagtat tcgtatgtgt acgtggatcg
     2941 gctcccacac gaagagatgg agagcgcgat ccgggaggtt gagaagcaca atccaagcgg
     3001 ttcattcccc accctggtca ttgacggttc cagggctatt gtcggtttca gggaagccga
     3061 tatccgggag gcgctgaaat gacaggagag ggaattcccg agcagaccat tcgtaaaaag
     3121 gccgataaga tccgatccga tgccagtgca ggaggttatc tcctcaaccc cgatcagtct
     3181 tttgtcaggg aactggttgc gggcatatgc gtgaatgaac agcgttacgg ttaccctgcc
     3241 tgtccatgtc gccttgcctc ggggagcagg gaggaggacc tcgacatcat ctgcccatgc
     3301 gattaccgtg acgccgatct gaatgagttt ggtgcctgtt actgcgcact gtacgtgtct
     3361 gcagggattg catcgggaga agcgcaggta acgtgcgtcc ccgaacggcg cccttcacga
     3421 aaggagagga gaaaggagtc gaggagcgcg cctgtttttg caggcgagtt gccggaacca
     3481 gtatggcggt gcagagtatg cggctacctg tgtgccaggg aaggcccgcc gcttgtctgc
     3541 cctatctgca aggcttcgca ggaccggttt gtccgtttca tctaatacct caaagaggac
     3601 atcgtgagtc cgggaactgg atgaaattgg tcactcggtc ccgcgccaga gcgcgatagc
     3661 acacactaat gtaggatact tcccaaaact gtacatcatg gcagatacca tttccatcga
     3721 agtggtgggg ctcaaagact cgacctgctc accatttccc tgcgatatga cgaggagctg
     3781 cgggctgtac gattgccatc ccaccggaaa gctggttccc gcattcgagg ccctgaaaaa
     3841 agagctggtt tcgcagtatg gcgaatcggt gcggatgaca ctcgtgctaa tcgatgaaga
     3901 catgcctgat catgtcaggg agatcattgc tacccattat ccgccaatcc cattcattct
     3961 catgaacggg gcgttgattc ccatgggcag gatctcgctg ccgttgatac aaaaagagat
     4021 agagaagata ctcccgatca ggtaatccgg gagagttcgc cattgtcaag gtcgtaataa
     4081 agaccgtgga cctggacctt cttctctttt attgccattt tcacgatggg atattgcatg
     4141 aggtgctcga tctggagcct gatgttctct ttctcgatca tctttgaacg tttcttcacc
     4201 tcttcagggg tctttggtgc cggaagcgta tcatcgacac ggtgtttcgc ctcgatggca
     4261 taattcagcc agagtggaat gtagacatcg tctgtttctt tatcgagtgc cttgatggcc
     4321 ccgcagtccg agtggccgca gacaacaata tccttcacct tgaggtgcct gattgcatat
     4381 tccagcacgg tcgcaaagtt ccagtcctgg agcggcacta tgttcccgat attcctgtgc
     4441 atgaagaggg tccctggcaa cgtctgggtg atatgtccgg tctggagcct cgaatctgag
     4501 catccgatcc agaggacctt cgggctctga cctttggtca gttcctggtg atgtgccctg
     4561 ttctccttga agaagccttc cctgaatttc ttattgcctt cgagcaattc gtcaatcatg
     4621 gtatatgcac ctgtgtcctt ccttcttcgt cacgatgagc gccgatcatg ggagcagtca
     4681 ctcccgatct ggatatggtg gtgcaaaggg attaaaggtt gatattttgc tctcggcgtt
     4741 cataccataa aagaagagaa tgtggttgag ttgaaagctc tcccggggat gatccggaaa
     4801 gccggtgcag cgtcatcgcg gttctgggag gtagattact gcaggggcgt cgcggtcgtc
     4861 atgatgattc tgttccacgc cgcgtttgac atgtggtacc tcggcgtcct ggacatccaa
     4921 gtccaatccg gcccgtggaa aatccttgca gtgtgcacgg cctctctctt tctcctcatt
     4981 gtcggcctct ctctctccat cagcagtgcg agggcacgaa aaacccttca caggagagaa
     5041 ttcatactca agtatcttcg caggggtgtg ggaatcatgg gccttggact cctgctcacc
     5101 ctggtgacga tggcaatggt cccaacagaa ccgatactct ttggaatact ccatctcatc
     5161 gggttatcag tgatgctggc cccgctctac gttcggtata catgggcaaa cctcgttgca
     5221 ggactggcgg tcattgcagt gggttggggg atcaaagggc tctatggtcc tgtctggctg
     5281 atatggctgg gggttcaccc gcagggtttt gcaagcctcg attacactcc catattcccc
     5341 tggctcgggg tggtactcct tggtgtgtgg ctaggccatc tcctctatcc aggcggcatg
     5401 agaagggctc ccctgcctgt ccctgacatg ccggcaaggg agatagtgtg ttttgcaggg
     5461 agacattcgc tgatgattta cctggtccat cagccgattc tcctccttgt catctcgatt
     5521 ctctggtccc tttggggaat cccgatcgta tgaggattgg gcattcaggg ttgcgccttc
     5581 tggaaggtca tacggaagac cacaccctgt gaatggtctc ccgcaacccg gtcgttgacc
     5641 gatatcgtgc caccgtatcg ttcaaggagt gtcttcacga tgtagagtcc aagcccttta
     5701 cctgcattct gtcggtcaga ctgtgagaaa cgcctgaata tcacggattt aaggtcatca
     5761 ggaattcccg ggccgttgtc tgcgacctcc accacaacga aggccgggtt ggtcgaatcg
     5821 acagatatgc agatgttcac ggcggaaccc ccgaacttga tgctgtttcc gatcaggttg
     5881 ttgaagatct ctccaatgag gtcatcagcc ctgaccatcg cgccggtctc ttcacactct
     5941 atacgcactt caggaaagag gcgcacctct ttttggatgg cagtgtccag ggtgactgcc
     6001 ctcagatcgt tttttcgcac ctgtatcttc ctgattgtct caaggttcct gataatctcc
     6061 ctgctcttct tgattccctc gatcatcttt gtggcgatct ctttcgattc accggacagc
     6121 atttccagga ggaggtcgcc gtaccatagt gaagcgagat ttgcattgtt gatatcgtgc
     6181 atcaggatat ccagatagag gttcgcctgc tggttggtct ctgtaattct ctgcgagagc
     6241 aggagccgtg taactgttgt cgcgatctcc cgggagaggg attcaagggt ctgctgctgg
     6301 tcttttgaac atgtccggct gctgcatgag aggaagtaca tgaccccaag ggcctcttct
     6361 ccgctctgaa gcgggaccac gatccctgaa gcgggagtat cgcactgggt ccctgattcc
     6421 tctttccata cgaggaattt cccttgcgag accgcttctt tcaatgcggc aactgactca
     6481 ttcccggatt ccatgagatt ttgatagagg gaatcatcac catggtggac ctttgcgaca
     6541 aggcgggaag atccttcctc atacagtgag attccgccgt actcgcaccc aaggtacctg
     6601 gtaagattcg caagcacacc tcttcccatc tcctccaccc cggttgcagc agcggcctct
     6661 gcgataacac ggttgatcgt tgagagctgc gtgttccttg cctcgatctc ctcttcgtgt
     6721 gctttccttg cggagatatc gatcacggtg agaaccatca tccgcccggg cagcctggac
     6781 ccagagatga gcacggggac agtggttcca ttctccctgg tcatcacact ctccatctgc
     6841 gatacggacc cgtcatcttt catggactgg aggagggaat ctctcgccag gtcgtctctc
     6901 cagaatacaa tgaggctttc acctttcagg tcatcggggt ggcgaccgat gagagttccg
     6961 ccgccggggt tcacttcttc tattctcaac tccgaatcgc cattctggac aaggaagatc
     7021 gaggcctctg aaccggagaa aatagccctg taacgcgcct ccctgtccct gaggccgctt
     7081 gaaagggacg agatcacaac ggcgattgca acgagcacgt agaaactggc cgtggtgatg
     7141 gcaaaagtga tatccgggag actcgggtac tggtaaaggt acacaaagaa aacctcagcg
     7201 atacccacgc caaccgcaaa gagcactcca cgcctgggaa accagtaggc ggccagtatg
     7261 attggcaggt aaaagagaag cggagtgatg gccgagaggc cgagcatggt gccagcgacg
     7321 tacccaatgc tgacgaggat agtcaccagc acgatgagga atacctgtgt ccttgagctt
     7381 atggtcagtt ctgatgtgct cttttgagtc attgcaggta tcacgttcac caatctattg
     7441 ctccgtttcc ttttaaatat ggatgcttgg caaccggaga gtgcgagtga aaagagaaga
     7501 ggaaagagcg ggttttagcg aaggcccttc tgggggtgca atcgcaatat ggtctgcgct
     7561 ttatcaaagg agcgcctggc ctcttcgaac ctgccaagtt ctttcaggca gactccccgg
     7621 tagttccagg cattcacatt gtcgggttct ttctgcagca caaggtcgaa acatgcgata
     7681 gcctgttcaa agagtgagaa attgcccgtc tcttttccaa gcttctcaag ggtctcaccc
     7741 ttggcataat aggttgcggg aagtttcccg ttcaattcca gggcgcggtt gaaacactgc
     7801 agcgcctcat cgtaccggcc gagttctttt aagcatatgc ccttctccgt ccaggcattc
     7861 acgtgagtgg gatccatctc gataacacgg tcaaagcagc tgaccgcatc ctggaacctg
     7921 ccgtggttgt aatagaggtc gactcctttc ttgtaccagc tcgcagtgga tttcccgaag
     7981 atgcggtcga acatgccgct ctccttctca cgcgctgcct gatccttctg tttgccctcg
     8041 gcacttcgga cttcagtgat gtcgcgtatg gactcgattg caccgattac cgtccccttc
     8101 cggtcagtga tcagcgtggc ccttgaccag aagactgcag gtttgccgtt gcgcaatccc
     8161 gtgggattct cggcaataat ggaattgccg tccctgcgga tgttctggta agagtgcgcc
     8221 ctaatctcgt cctcaggctt gttgatcagg tcgacgagga ggggtcttgg gctgtcgtaa
     8281 aaggggatag cgtattcgtg attgcctttt ccgatcatct cccgcgcagg aactccggtc
     8341 agatcttcca tggccttgtt ccatgataca acgaccccct ccctgtccgt cgcaaatgtc
     8401 gcatcaggga gatggttgat gatgtcaacc atccggcggt cggcgaggcg aagtgattcc
     8461 tcggcaattc ttcggttgat ggcctgccgg atcatcctgt cgagttcggc aaactgcgct
     8521 ttcgggtcct ggcctttcga gagatagaag tctgcacggt tgttgagggc gtctacgacg
     8581 acgtgctctc tgcccttccc ggtaaagatg atgaatggag tgttgtcacc ttcggcacgg
     8641 aggatcttga ggagcacgat cccgtccatc atcggcatct cgtagtcgga gatgatggca
     8701 tcgaatgacc tggttttcag gatttcaagg gcctcgaggg cagaccggca ggtctctacc
     8761 ctgatctcac cggacctctc gaggaaaagc cttgttatct caaggagggc cggttcgtca
     8821 tcgataaaaa gaacggatat catgccttcc cctgtgattg acatccccac ctgaaactcc
     8881 atttccattt ctttttactt aattctttca tttatttcct ctattcttac cactatcgct
     8941 actgcggggt gagtgaaaag tcatattgga ggtgagggag caaccgctgg gctgctctca
     9001 gcggaagagc gggacatccc tgaacacatc tgcatcgaac atgcccctgt cggtcactct
     9061 caggtgcgga atgaccgtga gcgaaaggaa agagaggtac ataaaagcgc ctggaatggc
     9121 cccaagatcc actgcctttc cattcagcgt cttcagcagc cctgcaacct cctcagcggg
     9181 cagagaggac atcagcccgc cgcaatcaag ggggaggagt tcgaggctgt cggggcctgc
     9241 aactgccatc cctcctccat tcctgatgat ccctcccaag gcctcgcgga ttgcctggtc
     9301 atccgctccc accgccacca ggttatgcgc atcatgtgca atgctccccg cgattgctcc
     9361 tttcttgagg ccaaacccat ggacgagacc aaggccgcac ctcccttccc ggtaacggtt
     9421 gcagaccacc accttcagga tgtcccggtc caggtccggg atctctgccc cttcaagttc
     9481 gaatgccagg tgctctgtca gtatctggtg cgggactatc ccgattaccc tggctgttcc
     9541 ctctcccttg attgccaaat cttcatgggt gggggcacga gccctcatcg gcgaagggat
     9601 aatccgtggt ttcttgtgga cagataggtc gagtatcttc ccggatttaa aggttttcag
     9661 gacagaaaac ggttcatcct ccaaaagaat gcagaagtct gcgttcctcc cgggtgcgag
     9721 tgcacccctg tcgtgaaggc cgaaacgatc cgcagctgag agagtcgcca tgcgaaggac
     9781 aatttcgagt tcaaggccgt attcgagcga cttccggatg caatcgtcga tatgcccttc
     9841 ttcaaccagc gaatcggcat gccggtcatc ggtggcaaac gctatccggg acgccgtgca
     9901 gggggatgcg agagggatga gttcgcggag gttcttttcc gttgaccctt ccctggcgaa
     9961 gatgaacata ccccggctca atttttccgc ggcctcctcc ttccccgtac attcgtggtc
    10021 gctctgcagc cctgcaagga tgtaggcatt gagggattct ccagagagga gcggtgcatg
    10081 tccgtcccga atggagcaga gcgcaagttt cgcgaaaacc ccttcgtccc gctccagtac
    10141 gccggggaca ttcatcactt ctccaaggcc cagcactcca tcccgcccga tgaactccga
    10201 gaggtcctcc gcagtcagtt cagctcctcc cctgtcaagg ggtgtggccg gcacacagga
    10261 aggaagcatg atgaagatgt ctaccggaag accttcccgc tcacggagca tgtactcgat
    10321 tcctgccttg ccgcagacgt ttgcgatctc gtgcggatcg gccacgacgc tcgtggtgcc
    10381 gtgcattgcc accacccgtg catactcgga ggggacaaga agggaacttt cgacatgtac
    10441 atgggcgtcg ataaggccgg ggaccaccct tcgtcccttc aggttgagag tatttctggc
    10501 cttgtaaaga ctgcccagtc ctacaatttt cccggatgcc actgcaatat ccgtttcaat
    10561 ccagccgcag gtgaacgggt cgtatacgca cgcattcttg aacagcgcgt cggccggttc
    10621 tttcccgagg gcctgtctca tcattgcagg agaggggaag tcttccatga gaaaaggagt
    10681 ggtccgggat ggcacaagaa cattcttctc aagagattgt gacccaggga cgccgcatcc
    10741 gatgaatgta gaaattagta gagaggggaa gggcaggtcc cgggtgaata gaagaggtaa
    10801 atgagacaga atgagcgatt gacgggacag ggaggttctt aaagaaatat ttaggtatta
    10861 cctgcgctct tcggcagcga tcttctgctg ggtggtcagt acatccacca ggaggtcttt
    10921 catgatcttc ttcatgaaat cctttccttc ctcggacgcg aggtaatcat ggaggttctc
    10981 tttgaactgt cccacatcgt tctgattgtc gacgaaatat attaatgctt gggtgataca
    11041 gtcagagacg gttgaaaact ttttctcctc gtataccagt ttattcactt tttcgtagat
    11101 atttgggggc attttgtaag aaatggccac ccgctctccc cgtttgttgt ttcccctatc
    11161 caccattcct aatgaaatgg agttccttcg tataaaaaag ctgcgaaaaa atgtgtatta
    11221 cctttgaaat accaattggg cgacacttta cctatgtatt acataaagaa tactttaaat
    11281 aggaacgcct gccatggtga tatagtggag gcagacaatc atgcacgaga tactgttctt
    11341 tgg
//
LOCUS       DAOQYR010000072        11113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_6308_length_11113_cov_8.138000, whole genome shotgun sequence.
ACCESSION   DAOQYR010000072 DAOQYR010000000
VERSION     DAOQYR010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 11113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11113
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6308_length_11113_cov_8.138000"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..1140
                     /locus_tag="PKM87_08665"
     CDS             7..1140
                     /locus_tag="PKM87_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNQ30176.1"
                     /translation="MNGSFKIGSIFGIPILIHWTFLVIIPLFAWIIGSQIVLTVELLS
                     GLFRVPIDMGYLTPGWNPYILGAFVALGLFGGVLVHEIAHCLVAKRMGVPINSITLLI
                     FGGVSSMEESTPDPKVELPMALVGPLTSLGVGLLCTALLYVFAGIIADPPLAGLLVFI
                     FAYLGLLNIFLFAFNLLPAFPMDGGRVLRAYLARKMPLHRATRIAANIGKMFAVIFGI
                     FGVIFFSPILILIAFFIYIGAGQESTAIKYNFLLKDVNVGDIMERQVMTVPPTMPVRE
                     VIDLMYSTKHLGFPVVDRGFVVGMITLADVHKVPPLDREAMQARDIMTREVIALAPGS
                     PVIEGLRLMSRYNIGRIPVMEHDHLVGIITRTDILKVIELKEI"
     gene            complement(1118..2791)
                     /locus_tag="PKM87_08670"
     CDS             complement(1118..2791)
                     /locus_tag="PKM87_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ30177.1"
                     /translation="MTGKSAFILDGYVDEPACLGVPPYISPYVRTVAGTLFAHGYSAG
                     YLTIDQVRKDPALIHRMHAADLVVMIAGVTVPGKYLGGTPATLTEIRLIGTQIRGPEK
                     LLAGPIGFGYSSGGGQRAVKDAVSGFGHLLTGSPASALDSFLREGKTHGSFNYRREDP
                     WAVAGAAIIRDHPSFPWVMCEIETARGCSRAHSGGCSFCTEPFYGPPAFRSIDGVAAE
                     IDALHRAGGLHFRLGRQPDILVYGSSTAEEYPTPVPGRLEELFSAVREAAPDLLTLHI
                     DNANPGTIARHPEESRAALDAIVAYHTPGDVAAFGMETADPAVIAENNLKAGPDQVMD
                     AIRIVNEAGGRREDGIPELLPGLNFIAGLAGETPLTFDLDREFLAAVLASGYLVRRVN
                     IRQLMPFEGTRAFSENALGVHKGLFAKFKDLVRKEFDLPMLAKVFPTGTILRSVVMEE
                     EGATSFGRQMGSYPILVGIPLRVPQRTLLDTVVVDHGMRSLTGLPVPIEVNLLPVATL
                     AWVPGVGRKRAGQIAAKRPFSNLAEFRAVAGPTPVDPCLSFSFKSPSAR"
     gene            complement(2788..3402)
                     /locus_tag="PKM87_08675"
     CDS             complement(2788..3402)
                     /locus_tag="PKM87_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNQ30178.1"
                     /translation="MNFDEWEPIYEEIVEYFGFDRKEDEDAADILEDLLERDDLDLLE
                     ETCRGHDVCVCGNAPCLVKEIPLVRGTVLAADAASLVLLSHGIRPDVIFTDLDGATDE
                     FLDMNREGTIVVVHAHGDNIPLVRYWVPRFPGPLVGTTQSRPLPHVHNFGGFTDGDRA
                     VFAAHSLGAAGITLLGFDLEDTRVDPMKRGKLYWAGRLLSLIEP"
     gene            complement(3392..4855)
                     /locus_tag="PKM87_08680"
     CDS             complement(3392..4855)
                     /locus_tag="PKM87_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNQ30179.1"
                     /translation="MPEAGGHVAGEKVKYAVLGCGSTGYHVATELSRKEGRVLVLDRD
                     EGRVKELRDQNVDAMIRDISDPGFLSGLPMFEIAFVLTGDHHSNLAAVRTLRQKLPLV
                     HIVARALDPIGAGTLESAGADFVLYPQQVVARAAIHQINRLHVSRLSQRLHDLLAGWE
                     GTLGIITHKNPDPDAIGSAMALAAIARNANPQKLACRIFYDGIIGHQENRTMVNLLDI
                     KMEKMEPQTIQDCTYLALVDCPAPGMNNTLSPRTRVHIVIDHHQDGHGLEGAMFVDVR
                     PGIGATASILSQYLQELDIPVDKNVATGLFYGIRSDTRDFRRNVTPQDLSNAAFLLPL
                     TDSDLLDQIMSPSLSQETLDVLGNAIRNRRIRSGYLFANVGFVRNRDSLPQAADLLIS
                     LEGVNTALVYGISDDAIIISSRNRDIRLHIGNVLEEAFGDIGDAGGHPNMAAATIPLS
                     YFRRVKNKEELLGLIMDPLLKKFTSLVGLDAEAGNEL"
     gene            5004..5077
                     /locus_tag="PKM87_08685"
     tRNA            5004..5077
                     /locus_tag="PKM87_08685"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5038..5040,aa:Lys,seq:ctt)
     gene            5187..6014
                     /locus_tag="PKM87_08690"
     CDS             5187..6014
                     /locus_tag="PKM87_08690"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ30180.1"
                     /translation="MNRCSDTHWTRRNTTLRAVIVLICGFSLLLAPAAAAPITASIST
                     SPSDNYQMKASGYEQEGMALMAVRDWNGLINLTSEGLSTYPDEAELMCLKAYALRKTG
                     HFQEAVDLLNVAIPLDPRPARYANRGYAFLAMGRTNEALEDAEQAILLNSSYSSGQGL
                     KAETLLAMGDFPAALASADAALALEPENAHYWHVRGNALAGLGDCSGAFVSLEYSISL
                     KNEYDLPWPGLPNASTDLARVRSACMMPQETPAPTQAALPALMVVAALVVAVAEKRK"
     gene            complement(6159..7046)
                     /locus_tag="PKM87_08695"
     CDS             complement(6159..7046)
                     /locus_tag="PKM87_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ30181.1"
                     /translation="MIEARNLTKCYGTTPALDHISFDLDRGQIFGVIGHNGAGKTTLL
                     KILAGLVEPTSGELVMDGVDILRSPDGMKQMIGYLPEESRLYETMRVDEYLSFFGEIY
                     GMDRSSIRARSRELLEWLALEPNGKKMGEFSKGMKRKAAIARSLMHDPSLLIYDEATS
                     GLDPMTSRHIVDFLRDLRDHGKTIVLSAHNLYQVEAICDRILILRRGKRVALGTMQEL
                     REMFGSIFYFVYFTVGDIGKVEGLSSYVMDGGYFRAKATSVEEMGRFSNAIVNAGGAV
                     ERIESHYPSLEEMLVKIGA"
     gene            7129..8946
                     /locus_tag="PKM87_08700"
     CDS             7129..8946
                     /locus_tag="PKM87_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HNQ30182.1"
                     /translation="MNVSQFLTRIGTLARWEVRRTFGVMGKSVLPMAVVLFVLLVAAT
                     GFTAERGLHLQDGIYLIGTDDRSVGEILSGDERFTIIIGDGNSLFRDRAGYDVIIISG
                     QAYVQNNDRGRSALKTLQRDYQQYLDSVYSLQEDLFAAYPLWIDLQEVKSELDFSATQ
                     AGMQISVAPSSTAAPVPEGPVVDIPAPEPGLALSTEELRQGLLLSQGEQSQLSRYSDL
                     IGPESPFGSFKTPSQLSPPLPFDSIILVFLFIFPLYFTSQFYMMSIMQERLERKGEVL
                     LSTPAGAPAIIFGKALPYFFGMLAISAGIALWLNASVSILLPLLPVILFFLANALIIG
                     MVSRSFKELSFISIFFSTVATSYLFFPSIFANVHVISLVSPLTLIVLTLQGETYTIGQ
                     YLFSTSLFFLTSAALFYVGIANFREERLFSQSGILSRIREFVSSSLSNRHPWVSLFAV
                     NTLMVPFVFMVQMLLLVLFFNLPMPLSLVLLIVSAAFVEEIAKSLGISTFLIENPARF
                     SWRWVVIGSAVTAAAFLFAEKLLLLVTLAQVTESIFGSILFLSSGALLLPFLLHFIGV
                     LCVGGLVRFRGARGYLPGILLATLVHCLYNGYFILGWLL"
     gene            8943..10022
                     /locus_tag="PKM87_08705"
     CDS             8943..10022
                     /locus_tag="PKM87_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ30183.1"
                     /translation="MNTRNAALVMKKDLRGLSRERTIVLAIMLQLFVALFSSFLMVGL
                     TSMYDPSALARFSGVKYQVGYVGESPELENLLRESNQFQVFPMDLSTAVAALGERKLS
                     AVIHVPDTAIDAEEPVKITLYTLQNDIQSTIVNVKLKEVFLDYEEELRQVRSGRLDLI
                     PIPLEFPASRAGPGFYEFIYGLLIPLLLFMPAIISAALIIDLITEEYQHHTLETLLST
                     PMTMADVVWGKVAACVVLVPLQAGTWILLLALNRIHIQNILQILAHVISFSLVLVLIG
                     ALTALYYRERTAAQFIFSTTVVVVMLVVLAFPGNPLNLIALLATGAAGMGQWSVLGVT
                     LLFCCGLGLLTHVYAGRVSTRAPGE"
     gene            10178..10816
                     /locus_tag="PKM87_08710"
     CDS             10178..10816
                     /locus_tag="PKM87_08710"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNQ30184.1"
                     /translation="MYCPKCGRETESGGRYCQWCGADTRVAPPRAVLRKKVGDISTEK
                     YAGLGRRIGSGLIDILFLILFDMIAVGAISIISWFLQRPCPLSESIVMLYQYYRHVPR
                     TDAYGNVVNAAIPSQIVFSAGILFLLIPWIYFAWLESSRNQATLGKMVARIAVTDMQG
                     SRITFARGTLRFFAMSLSVLTLCVGFIIPAFTRYKQGLHDIIAGTLMFRQDP"
BASE COUNT         2180 a         3328 c         3081 g         2524 t
ORIGIN      
        1 cttttaatga acgggtcgtt caaaatcggg tccattttcg gcattcccat ccttatccac
       61 tggacattcc tcgtgatcat ccccctgttt gcctggatca tcgggagcca gatagttctt
      121 acggtcgagc ttctctcggg actcttccgt gtcccgatcg atatgggcta ccttacgccg
      181 ggctggaacc cttatatcct gggggccttc gtggcgctcg ggctctttgg cggtgtgctg
      241 gttcatgaga ttgcacattg cctggttgca aagaggatgg gcgtccccat caacagcatc
      301 accctcctca tatttggggg agtctcatca atggaggaga gtactcccga tccaaaggtg
      361 gagctcccca tggcactcgt ggggccgctc accagccttg gagtgggcct gttatgcacc
      421 gccctgctgt atgttttcgc gggcatcatt gcggaccctc cactcgcggg gctgctggtc
      481 ttcatctttg cgtacctggg gctcctcaac atcttcctgt ttgccttcaa cctccttccg
      541 gcgtttccca tggacggagg gagggtgctg agggcgtatc tggcccggaa gatgccgctt
      601 caccgtgcaa cccgtatcgc ggcaaacatc ggaaagatgt ttgcggtcat cttcgggata
      661 ttcggcgtca tttttttctc ccctatcctt atcctcatcg cgttcttcat ctatatcggg
      721 gccgggcagg agtctacggc gattaagtac aacttcctgc taaaagacgt gaatgtgggc
      781 gatattatgg aacggcaggt gatgaccgtc ccgccgacca tgccggtcag ggaggtgatc
      841 gatctcatgt actccaccaa gcacctcggg tttccggttg tggatcgtgg gttcgtcgtg
      901 gggatgatca ccctggccga cgtgcacaag gtccctcccc tggacaggga agcgatgcag
      961 gcaagggaca tcatgacacg cgaggtgatt gcactggccc ccgggtcgcc tgtgatcgaa
     1021 gggctgcggc tgatgtcccg ctataatatc ggccggatcc ccgtgatgga acatgaccat
     1081 ctggtcggca tcatcacccg gacagatatc ctgaaggtca tcgagctgaa ggagatttaa
     1141 aagagaagga gaggcaggga tcgacagggg tcggcccggc gacagcccgg aactccgcaa
     1201 ggttggagaa gggtctcttt gctgcgatct gccctgccct cttcctcccc acgccaggga
     1261 cccaggcaag cgttgcaacc ggcaacaggt tcacctcgat cgggacgggg agcccggtca
     1321 gggatctcat cccatggtca accactaccg tgtcgaggag agttcgttga gggacgcgga
     1381 gcgggatccc cacgagtatc gggtacgagc ccatctgccg gccaaaagac gtggcgccct
     1441 cctcctccat caccaccgag cgaaggatcg tccctgtggg aaacaccttt gcgagcatgg
     1501 gaaggtcaaa ctctttcctg acaaggtcct tgaacttcgc gaacagcccc ttgtggaccc
     1561 cgagcgcgtt ctccgagaat gcccgcgttc cctcaaatgg catcagctgc cgaatattca
     1621 ccctgcggac aaggtatcct gatgcaagca ccgctgccag gaactccctg tcaaggtcaa
     1681 aggtcagcgg ggtctccccg gcaagccctg cgatgaagtt gagccccggc aggagctcgg
     1741 ggattccgtc ctcgcgtctc ccgccagcct cgttgacgat ccggatcgca tccatcacct
     1801 ggtccggacc ggccttcaaa ttgttctctg caatcaccgc cgggtccgcg gtctccatcc
     1861 cgaaggccgc aacgtccccc ggggtatggt aggccacgat cgcgtcaaga gccgccctgc
     1921 tctcctccgg gtgccgtgca atagtccccg ggttggcatt gtcgatgtgg agggtgagga
     1981 gatcaggcgc cgcttccctg accgcggaga agagttcctc caacctcccg gggacaggcg
     2041 tcgggtactc ttctgccgtc gatgacccgt agaccaggat atccggctgc ctcccgaggc
     2101 gaaaatgcaa cccgccggcc ctgtgaaggg cgtcgatctc ggcagcaacc ccatcgattg
     2161 agcggaatgc gggcggcccg tagaacggct cggtgcagaa ggaacacccc cccgagtgtg
     2221 cccgggaaca tccccgtgcc gtctcgatct cgcacatgac ccacgggaag gagggatgat
     2281 ccctgatgat cgcggcaccg gcaaccgccc atgggtcctc gcggcggtag ttaaatgaac
     2341 cgtgggtctt tccctcgcgg aggaatgagt cgagggcgga ggcgggagat cctgtgagga
     2401 ggtggccgaa tcccgagacc gcatctttca ctgcccgctg ccctcctccg gaggagtacc
     2461 cgaatccgat ggggcccgcc aggagttttt cgggaccacg tatctgtgta cctatcagcc
     2521 ggatctcagt cagcgtggcg ggcgtccccc caaggtactt cccgggaaca gtcacgccgg
     2581 caatcatcac caccaggtcc gcggcgtgca tcctgtgaat gagggcgggg tccttccgta
     2641 cctggtcgat ggtcaggtat cctgccgaat atccatgcgc gaagagggtt cctgcaacgg
     2701 tccttacata aggcgagata tagggaggaa ctccgaggca cgccggttca tcgacatagc
     2761 cgtccaggat gaacgcggac ttcccagtca tggttcgatc agggagagga gccttccggc
     2821 ccagtacagc ttccccctct tcataggatc gacgcgggtg tcctcaaggt cgaacccgag
     2881 gagggtgatg cctgcggcac ccagggaatg cgcggcaaag accgcacggt ctccgtcggt
     2941 gaaaccgccg aagttgtgca cgtggggaag ggggcggctc tgggtggtcc cgacaagcgg
     3001 gcctggaaac ctcggcaccc agtaacggac gaggggaatg ttgtcaccgt gggcgtgcac
     3061 gaccacgatt gtcccttccc tgttcatatc caaaaattcg tctgtcgccc cgtccaggtc
     3121 agtgaagata acatcggggc ggatcccgtg ggaaagaagg acaagagagg cggcatcggc
     3181 tgcaaggaca gttcccctca ccagggggat ctcttttaca aggcaggggg catttccgca
     3241 tacgcagacg tcatgtcccc ggcaggtttc ttcgagcagg tcgaggtcgt ctctctcaag
     3301 gagatcttca aggatatcgg ccgcatcctc gtcttctttc cgatcgaaac caaaatactc
     3361 gacgatctct tcgtaaatcg gttcccattc atcaaagttc atttccggcc tctgcgtcca
     3421 ggcctacgag gctggtgaac ttcttgagga ggggatccat gatcaggccg aggagttctt
     3481 ccttgttctt cacccgccgg aaataggaga gggggatggt tgcggcggcc atattggggt
     3541 ggcccccggc atcgccgata tccccaaatg cctcttcgag gacattcccg atatgaagcc
     3601 gtatatcccg gttccgtgag gagatgatga tggcatcatc ggatattcca tacacaagcg
     3661 cggtgttcac cccctcaagg gagatcagga gatctgctgc ctgaggcagg gagtcgcggt
     3721 tccgtacaaa tccgacattc gcaaagaggt acccgctcct gattcgccgg ttccggatgg
     3781 catttccgag cacgtcaagg gtttcctgcg aaagagaagg ggacatgatc tggtcgagca
     3841 ggtcagagtc agtgagcggg aggagaaacg cggcgttcga gaggtcctgt ggggtgacgt
     3901 tccggcggaa gtccctcgta tcggaacgta ttccatagaa aaggccggtg gcaacattct
     3961 tgtcgaccgg gatatcgagt tcctggaggt actgggagag gatgcttgcg gtggcgccga
     4021 tccccggcct cacgtcgacg aacatcgccc cctcgaggcc atggccgtcc tggtggtggt
     4081 cgatcacgat atgcacccgc gtccttggcg agagggtgtt gttcatcccg ggcgcggggc
     4141 agtcgacgag ggcaaggtag gtgcagtcct gaatggtctg gggctccatc ttctccatct
     4201 tgatatcgag gaggttgacc attgtgcggt tctcctggtg cccgatgatc ccgtcataga
     4261 agatccggca ggcaagcttc tggggattcg cgttccgggc gatggctgca agggccatcg
     4321 cgcttccgat cgcgtcaggg tcggggttct tgtgcgtgat gattccaaga gtcccctccc
     4381 accccgcaag gagatcgtgg aggcgctgcg agagccggct gacatggagc cggttgattt
     4441 gatggattgc ggccctggcc accacctgct gcggatagag gacgaagtct gcccctgctg
     4501 attcgagcgt tcctgcaccg attgggtcga gcgcacgggc cacgatgtgc acaagcggca
     4561 gtttctggcg gagcgtcctg actgcagcga ggttcgagtg gtggtccccg gtgaggacaa
     4621 atgcgatctc gaacatgggg agcccggaga ggaacccagg gtcagagata tcccgtatca
     4681 tggcgtcaac gttctggtcc cggagctcct ttacccgtcc ctcgtcgcga tccagcacaa
     4741 ggacccggcc ttcttttcgc gacagttccg tggccacgtg gtaccccgtg ctcccgcatc
     4801 cgaggaccgc atatttcacc ttctctcctg ccacatgtcc gcccgcctct ggcatgctac
     4861 aatgtcactc cttgatgata ttaaggttac aacctgaaac ggggagattg cgcgttggaa
     4921 actgccgcat gggggggtta ctggcagaaa aataaaaaca tttatactca attcggaacc
     4981 atgaatatag gccgatcagg catgggcttg tagcttagtt cggaagagcg acggactctt
     5041 aatccgtagg tcaagggttc aaatcccttc aggcccgctt tctttttcta ttgtatctgt
     5101 tcatgcagtg ttttgatttt tggccaagcc attccatgag atatcatgta caaatctcta
     5161 taagataccc aatcccctga aatgatatga accgttgttc agatactcat tggacccggc
     5221 ggaataccac gctccgggct gttattgtct taatctgcgg tttttctctc ctgctggcac
     5281 cggcagccgc tgcccccatc accgcatcca tctcaacgag cccgtcagac aattatcaaa
     5341 tgaaggcttc aggttacgag caggagggca tggcgctgat ggccgtgcgt gactggaacg
     5401 gacttatcaa tctcaccagc gagggtcttt caacctatcc tgatgaggcc gaactgatgt
     5461 gcctgaaggc gtatgccctc aggaagaccg gacatttcca ggaagccgtg gaccttctca
     5521 acgtggcgat accgcttgac ccaaggccgg cacggtatgc aaacagggga tacgcgttcc
     5581 tcgcgatggg aaggactaat gaagcactgg aggacgcaga acaggccatc ctgctcaatt
     5641 cttcttattc ctcgggccaa ggattgaaag cagaaactct ccttgcaatg ggggactttc
     5701 ctgctgcact tgcatcagcc gatgccgccc tcgcccttga gccggaaaac gctcattact
     5761 ggcacgtacg tggaaacgcg ctcgccggtc tgggtgactg cagcggggct tttgtatcgc
     5821 tcgaatattc catttcattg aaaaacgagt atgatttacc gtggcccggg cttccaaatg
     5881 cgagcacgga cctcgcacgg gtgcgttctg cctgtatgat gccacaggaa acacccgccc
     5941 cgacacaagc ggcacttccc gccctgatgg tggttgctgc gttggtggtc gcggttgcag
     6001 aaaagcgaaa atagggaacg tgcaattaca aacctgtctc cggattctga agaggagatg
     6061 tgggttggaa tgcccgcttc ctgaaacata tctcgagtat ctgtgacatt caaagatgaa
     6121 gaaaacaaat tgggccaggt caaagagatc tgccggtttt atgctcctat cttcaccagc
     6181 atttcttcga ggctcgggta gtgggactcg atccgttcca ccgctccgcc ggcgttcacg
     6241 atcgcgttcg agaaccttcc catctcctcg acgctcgtcg cctttgcccg gaaatacccc
     6301 ccatccatga catacgatga gaggccctcg acctttccga tgtccccgac agtgaagtac
     6361 acaaagtaga agatcgagcc gaacatctcc cgcagctcct gcattgtgcc gagcgcgaca
     6421 cgctttcccc tccggaggat gaggatcctg tcgcagatcg cctctacctg gtagaggtta
     6481 tgggccgaga ggacgatcgt cttgccatgg tcccggaggt ccctgaggaa gtcaacaatg
     6541 tgccgggagg tcatggggtc aagccctgaa gtcgcctcgt cgtagataag gagggatgga
     6601 tcgtgcatca gggatcgggc gatcgcggcc ttccgcttca tccccttcga aaactccccc
     6661 atcttctttc cgttcggctc gagcgcaagc cattccagga gttcgcggct gcgtgcgcgt
     6721 attgacgacc ggtccatgcc gtagatctcc ccgaagaatg agaggtattc gtccaccctc
     6781 atcgtctcgt acaggcgtga ctcttcgggg aggtagccga tcatctgttt catcccgtcg
     6841 ggactgcgaa ggatatcgac tccatccatg acaagttccc cgctggtggg ctcgacgagt
     6901 cccgcaagga tcttgagaag agtggtcttt cccgccccgt tgtgtcctat gaccccgaat
     6961 atctgcccgc gatcaaggtc gaagctgatg tgatcgagtg caggggtggt gccgtagcac
     7021 ttggtgagat tgcgggcttc gatcatggat tccttgtcca atcctttaca attcctcgac
     7081 ctgcaaggta ttataggtac tgaacctacc ataaaagagg cacaacgcgt gaacgtttcg
     7141 cagtttctca cacggatcgg gacgcttgca cgctgggagg taaggcggac tttcggggtc
     7201 atgggaaaga gcgtcctccc catggcagtt gtcctctttg tccttctcgt ggcagcgacc
     7261 ggctttaccg ccgagcgggg cctccacctc caggatggga tctacctgat aggcacagac
     7321 gaccggtccg ttggtgagat cctctccgga gacgaacggt tcaccatcat tatcggtgac
     7381 ggaaactcgc ttttccggga cagggctggg tatgacgtca tcatcatcag cgggcaggcg
     7441 tatgtccaga acaacgatcg cgggagatcg gcgctgaaga cgctgcagcg ggactaccag
     7501 cagtatcttg attcggtata cagtctccag gaagacctct ttgccgccta tcctctatgg
     7561 atcgacctgc aggaggtgaa gagcgaactc gacttctccg caacccaggc agggatgcag
     7621 atctctgttg caccctcctc gaccgccgcg ccggtcccag aagggcctgt cgtggatatc
     7681 cccgcaccag aacccggcct tgcactctcc acggaagaac tgcgtcaggg gcttctcttg
     7741 tcccagggcg agcagagcca gctttcccgg tactctgacc tcatcgggcc agagagcccg
     7801 tttgggtcat tcaagacccc ctcgcagctc tccccacctt tgccattcga ttcgatcatt
     7861 ctcgtcttcc tcttcatctt ccccctctac ttcacctcgc agttctacat gatgagcatc
     7921 atgcaggagc gtctggaacg caaaggagaa gtcctcctct cgacccctgc aggggcgccc
     7981 gcaatcatct tcggaaaggc tcttccctat tttttcggga tgcttgcgat ctcggccggg
     8041 atcgcgctct ggctcaatgc ttccgtctca atcctccttc ctctcctccc tgtcatcctc
     8101 tttttccttg caaatgcgct gatcatcggg atggtctcca ggagcttcaa ggaactctct
     8161 ttcatctcga tcttcttctc gaccgtcgca acatcctacc tcttcttccc cagcatcttc
     8221 gccaacgtcc atgtcatcag ccttgtctca cccctgaccc tgattgtcct cacactccag
     8281 ggcgagactt atacaatcgg gcaatacctc ttctccacct ccctcttctt cctcaccagt
     8341 gcggccctgt tctacgtcgg catcgcaaat ttccgcgagg aacggctctt ctcccaatca
     8401 ggtattctat cacggataag ggagtttgtg tccagctcgc tctccaaccg tcatccatgg
     8461 gtctcccttt ttgcggtaaa taccctgatg gtgccgttcg tcttcatggt ccagatgctc
     8521 cttctcgtcc tctttttcaa cctccccatg ccgctctcgc ttgtcctcct catcgtatcc
     8581 gcggcatttg tggaggagat cgcaaagtct cttggcatca gcactttcct catcgagaat
     8641 cctgcccgct tctcgtggag atgggtggtt atcggctcag ccgtcaccgc ggcagccttc
     8701 ctctttgccg agaaactcct cctcctcgtc accctcgcac aggtgaccga gagcatattc
     8761 gggagtatcc tctttctctc atcgggagcg ctcctcctcc cgttccttct ccactttatc
     8821 ggagtcctct gcgtgggagg cctcgtccgg tttagaggtg cgcgcggcta tctccccggt
     8881 atcctgctcg caactcttgt ccactgtctc tacaacggct atttcatcct ggggtggctg
     8941 ctgtgaacac gaggaatgca gccctggtga tgaagaagga tctccggggt ctttcgcggg
     9001 agcggacgat tgtccttgcc atcatgctcc agctctttgt cgcactcttc tcctcgttcc
     9061 tgatggtagg gctcacctcg atgtatgacc cctctgctct cgcccgcttc tcgggagtga
     9121 agtaccaggt cgggtacgtg ggggagtccc ctgaactcga aaaccttcta agggagagca
     9181 accagttcca ggtcttcccg atggatctct ccactgcagt tgccgcgctc ggggaacgga
     9241 aactctctgc agtgatccac gtgcctgaca ccgcgataga tgcagaagag ccggtaaaga
     9301 ttactctcta caccctccag aacgatatcc agtccaccat agtgaacgtg aaattaaagg
     9361 aggtcttcct ggactacgag gaggagctcc gtcaggtgcg ctccggcagg ctcgatctca
     9421 ttcccatccc cctggagttc cctgcaagca gggcagggcc cggtttctac gagttcatct
     9481 atggtctctt gatacccctt ctcctcttca tgccggccat catatccgcg gcgctcatca
     9541 tcgacctcat caccgaggag taccagcacc acaccctcga gaccctcctc tccaccccga
     9601 tgaccatggc cgatgtggtg tggggcaagg tcgcggcctg tgtcgtgctc gttccattgc
     9661 aggccgggac atggatcctt ctccttgcac tcaaccgcat ccatatccag aacattctcc
     9721 agattctggc ccacgtgatc tccttttcac tcgtgctggt ccttatcggg gccctcactg
     9781 ccctttatta cagggagagg accgccgccc agttcatatt ctcgaccacc gtggtggtgg
     9841 tgatgctcgt tgtgctggca ttccccggca acccgctgaa cctgattgca ctgcttgcaa
     9901 ccggcgcagc ggggatggga caatggagcg ttctcggggt gactctcctt ttctgctgcg
     9961 gcctcggcct tctcacccac gtgtatgccg ggcgggtctc cacccgggcg ccgggtgagt
    10021 agccgccaca gggtttttcc cccaattttg ggtcgcgaac agaaaaaaac gacaaaatgc
    10081 actttatttg ctttttcttc cctgttgaga cagagtattt cgatacattg aagtcactcg
    10141 catcgattac taccattacg aagaaggagc gggaaggatg tactgtccca agtgcggaag
    10201 ggagacggag agcgggggga gatactgcca gtggtgcggt gcggatacgc gcgtcgcgcc
    10261 gccaagggct gtcctccgga agaaagtcgg agatatcagc actgagaaat acgccggcct
    10321 ggggagaagg attggttctg gtttgatcga tatcctcttc ctcatcttat tcgacatgat
    10381 cgcggtcggc gcgatctcca tcatctcgtg gttcctgcag aggccctgcc ctctctccga
    10441 gtccatcgtg atgctctacc agtattaccg ccatgtcccc cgcacagatg cgtacggaaa
    10501 tgtcgtgaac gccgccatac cctcccagat cgtcttctcc gccggaatac tctttctttt
    10561 gattccctgg atctactttg cctggcttga aagctccaga aaccaggcaa cgctcgggaa
    10621 aatggtggca cgaatcgccg tgaccgatat gcaggggagc cgtatcactt ttgcacgggg
    10681 aacgcttcgt ttcttcgcga tgtcgctctc agtcctcaca ctctgcgtgg gattcatcat
    10741 cccggccttc acccgttaca agcagggact gcacgacatt atcgcaggga cactgatgtt
    10801 ccggcaggac ccataacatc cagtgtcccc tccaggaacc cccatacgga acctggtgcg
    10861 ggactcatat catcatgcgc gcgatgcaaa acacaatttt tccgggtatc cacgcgatga
    10921 cccggtatga gggacaggtg agaatgaaac aagggtgttt cggcatggca tgccggtgat
    10981 accgcatggc tgcgtcctcc ccgctctcca gactctccct tcgctcttct ctcatactgt
    11041 gtatcaccgc gatcatcctc attgtgatca tgttcctgat ggccgccgca tatgcgcagg
    11101 cctattcatg gac
//
LOCUS       DAOQYR010000073        10883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_6476_length_10883_cov_5.884651, whole genome shotgun sequence.
ACCESSION   DAOQYR010000073 DAOQYR010000000
VERSION     DAOQYR010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 10883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10883
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6476_length_10883_cov_5.884651"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3986)
                     /locus_tag="PKM87_08715"
     CDS             complement(<1..3986)
                     /locus_tag="PKM87_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ30185.1"
                     /translation="MNRMFSPIPAMAERTWKAVIVLLSVAVISLTVYCLSQGITIIFM
                     HLYYIPIVLLAYRYRRQGLWGIVFLSFLYLVLVVLFHPADLEIIEGAVIRTLVFIGIG
                     VLVVYLAENLERIRSDLERSQQVQRRIIQNANVWMMVLDEKGRVIEWNPAAELMCGYT
                     FEEVRGRNEVWKALYPDQEYRREILRRINGIISTKSHLENFSTTVTCRGGHTKTILWN
                     TREISHREGEPPLYIGVGVDITEQKRAEEEVTLQAQRTQNLLELNRMKDVPFRDLLGY
                     SLEACLHMTESGYSFIGLISPDESVMTIHSWSKDAMKECTVSDKPMHFPIAAAGVWGE
                     AIRQRTPFILNDYSLAHPAKHGYPEGHVPITRYLGVPIFDGELIVAILAVANKPEDYD
                     EGDINALTTLGNHLWELLHRRQAEEALQFSNIILSTQQEVSLDGILIIDEWGKIISYN
                     QRFADMWNIPPDVLATHSDEQAMQEVLALLVEPEKFLDRVRYLYEHRDQKSREEIALT
                     DGRTFDRYSASITGKDGKYYGRVWYFRDITEQKRAANALIENEKLLTEVGRLARVGGL
                     ELDTGTGKIRWTRETYVLHEVYDRDHLELDEALGFFDEPDRIRIREAIQRCTATGEMI
                     DLEVPLTSAKGRRLWVHITGRAVKEGEKTGRLIGAIQDITELKEARDALLQSEEQYRG
                     VAERISDLLLLVDQAGTITYSSPSIGRILGYRPEEVVGTAAFDYVHPESMQSVSEAVD
                     RNLGGVHTEGLEAHVRKNDGSYAVMEMSFAPVIRSGAVDGIQVIGRDVTEKRAAQERI
                     AELLKIHEEEVRVINTSPAVAFLWRAEEGWPVETVSENISQFGYFPEEFLSGRIAFRD
                     IIHPDDLERVNGEVAYNSEHGIDEYIQQYRILGKDGKECWIEDYTHIRRDNEGRITHY
                     EGVILDITRRKRAEDALRRSEQRNARLLEAVPDLMFTISRDGVYLEFHVPDRSVLAIP
                     ADQILGRNVRDTGFSEEMTELMMERIARAIDTKELQQFEYDLSLPGGLRHYEARMIAL
                     SQEEVLAIVRDVTDQKALQTALRTSEEKYRLLIEKAEEGVWMVDRDYRTTFVNARLAE
                     MFGYRPEEMWGRRVVEFLPESSRETHEKRVRERLQGKSERFEQQFIRKNGSLFWVSAS
                     TSPIVEKGEIIGAFSLLTDIDDRKYAEELQKRFTEELEQQVVARTQALNASLQEKVVL
                     LREVHHRVKNNLQIIISLMNLQMRKAEDPRLRDLLLETQDRVRAMSLVHEKLYQSEDL
                     SHINLSTYLHSLGTQLISSHGDIARKITLRMGFDPIMTDINIAIPLGLVVNELVSNAL
                     KHAFPGEMT"
     gene            complement(4045..6198)
                     /locus_tag="PKM87_08720"
     CDS             complement(4045..6198)
                     /locus_tag="PKM87_08720"
                     /inference="COORDINATES: protein motif:HMM:NF025774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HNQ30186.1"
                     /translation="MAHAHGPIKQISDIRPGDHLCCIYSTDEEHREVLTRFLLDGLKN
                     HEKVFYIVDARTAETIMGYLKDAGLDVDSYLRTGQFSILTVSDSYMKDGVFDPDRMIA
                     LLSTETENALQEGYSALRVTGEMSWALGGLPGSERLIEYEHKLNTFFPGSRCVAVCQY
                     DRRRFDQDILLHVLMTHPFAFIGEHLYENFYFTPPEEFLYPGREKTTLDRWISNLKDR
                     KEAEEALRRSEERFRALFDHMLEGFALCRMIYDEGGRPVDWEYLEVNPAFGRLTGLRD
                     IAGKKVSEAIPGTKEQTPEIFEIYGRVATTGRPETFDVFFTPLRIWLHISVSCPQKGY
                     FVAVFQDITSQKESEESLRKSEETFRSYIENAPDGIFLADAEGNYLMVNEAACAITGY
                     SKTELVGKNIRDLIPPGDQQIAFAHFRQVKEEGQATGETRYVTKSGEVRYWLVKAVRI
                     DDQRLLGFTSDITDRKMMESEIRSLNTVLEQKVEERTHALADTNVALEEEITRRREME
                     DQIRAALNEKTLLLREVHHRVKNNLQIIISLSNLQLRQIEDPKMRQVMAETQNRVRAM
                     ALVHEKLYKSETLSDIDLAEYVRFLTSQLFAYYETDSRKVTLKTEIGKITLPINTAIP
                     LGLIINELVSNALKHAFPDGRAGSLFILARREEKTINLVVEDTGIGIPADLEWRNTQS
                     LGLKLVISLVNQLDGTIDLDRTRGTAFTMVLKVKE"
     gene            complement(6268..>10883)
                     /locus_tag="PKM87_08725"
     CDS             complement(6268..>10883)
                     /locus_tag="PKM87_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ30187.1"
                     /translation="DILDFLPDATFAIDAEGRVIAWNRAIEAMTGVPSDQIIGRGDYE
                     YSIPFYGERRPILIDLVLKSRDNIEQKYLYIQRDGDKITSETFIPDIFEGRGAHLWGT
                     ASPLYDLHGNLAGAVEVIRDITERKESERALIKSEKLLNQTQQYAHVGGWEYDILSGT
                     ITWTDEVYRIYEVPVDFDPNNIQEDLAFYSPEDRSILEDAFHRAVEEGIPYNLEMRFV
                     SGTGVKKWVKTIAQVECDDGRPIRLIGTIVDITDKKEAEIRVSELLESARNEKERLST
                     LLNSITDEVWFTDTDHRFTLANPQALEEFQLREGDTPIVEDLARSLEVLRPDGTPRPV
                     EEAPPLRALSGEVIRNMEEIIRTPVTGEFRYRQISSTPVRDTTGAIIGSVSVVRDITN
                     QKRAEEEIRESNERFKIVSKATNDTVWDWDLLTDSQWWNEGIQSVFGYRPEEIEPTVA
                     SWYTRIHPDDRERIIDGIHAAKDGGENKWSDEYRFRKADGTYAYVFDRGFVIRDPEGR
                     AIRMVGAIQDLTERMRMVDALRESEERFRHILEDQTEFICRFRPDGTYVFVNDAYARY
                     FAKTREELIGHIYRPVVFPEDRQIVQDVFDSLSQEHPVATSEHRITMPDGRVRWQQWS
                     DRAIFDETGAVREYQSVGRDITAQKEAEEALKQSEARFRNLFSNMSAGVAIYGVVEDG
                     KDFIFREINPAVERIENVRRADVIGKSVLEVFPGVKEFGLFEVFQRVWRTGIPEQHPV
                     SEYRDGRISGWRENTIYRLPSGEVVSIYEDVTGKKQAEEAIRLSEARYRTLTEGIPDI
                     IARFDREHRHTYINHAIERVTGKPAESFLGRTNQDLGMPDELNRLWDENLDRVFEEGS
                     PVTFEFVYPSGENPVVFESRIIPEFSQSGEVLSVISVARDISAEKRADKKRKESESRL
                     DVAMEMGNIAWWEMDLPSGGVRFHDRKATMLGYAPGQFATYQDFCALIYPDDLDRTMQ
                     AMRDHLEGKVPRYDVEYRIRTSGGEWRWFHDSGGVTRRDPDGKPATVTGMVVDITGIK
                     MAEEALREAEERYEEIVTHALDSIFIIDVTADERFRIRYMNPVSEDEMGITNASASGR
                     FIEDLFPGETSEHLSSMYRQCVKTGSPLSYEETMDRGPGRRYYSTTLIPLKGAGKRTE
                     RIIGISHDITNRKLMESEIRALNTVLEQRVIERTRELERTNLALIAEIGQREVAEEKL
                     RETNEYLNNLFDYANAPIIVWDPEFRITRFNHAFEWLTGCPSAEVIGQPVSVLFPEQS
                     RERSMAYLRQAMEGERWEAVEIPIHGREGIERIVLWDSATLLAGDGKTVVAAIAQGQD
                     ITERKNAEEKLRTSLDEKLTLLKEVHHRVKNNLQIIVSLLNLQSRYITDEKTLAAIRE
                     SQNRVRAMALVHEKLYQTENLSQINLDDYLKYLGKSLFQFYGAGTRGITFSTDAAGIH
                     VDINTAIPFGLIMNELISNSLKYGFPERRRGEVSISVRREDRALYVEYRDTGVGIPDG
                     LDWQDTKSLGLRLVNSLVSQLNGSIALDRREGTAFSMVLYEKE"
BASE COUNT         2025 a         3262 c         2951 g         2645 t
ORIGIN      
        1 gtcatttcgc cggggaacgc atgcttcagg gcattcgaga ccagctcgtt gacgacaagg
       61 ccgaggggga tggcgatatt aatgtctgtc atgataggat cgaatcccat acgtaaagtt
      121 attttccgtg cgatgtcccc gtgagatgag atcagttgcg tgcccagaga gtggagatag
      181 gtggagaggt tgatgtgcga gaggtcttcg gactgataga gcttctcgtg tacaagagac
      241 atcgcccgca cccggtcctg ggtctccagc aggagatccc ggagcctggg gtcctcggcc
      301 tttcgcatct ggaggttcat caggcttatg attatctgga ggttgttctt gacccggtga
      361 tgcacctccc gcagcagcac caccttctcc tggagggagg cgttcagggc ctgtgtgcgg
      421 gcgaccacct gctgctccag ttcctccgtg aagcgcttct gcagctcttc ggcatatttc
      481 cggtcgtcaa tatcggtaag gagcgagaag gcgccgatga tctctccttt ctccacgatc
      541 gggctcgtgg atgcgcttac ccagaagaga gagccattct tccggatgaa ctgctgctcg
      601 aagcgctcgc tcttcccctg caggcgttcc ctgacccgct tctcgtgtgt ctccctggat
      661 gactcgggga gaaattcgac aaccctcctt ccccacatct cctcgggccg atacccgaac
      721 atctccgcga gtcgggcgtt gacaaaggtg gtccggtagt ccctgtcgac catccacacc
      781 ccctcctccg ccttctcgat cagcagccgg tatttctcct cgctcgtgcg gagcgccgtc
      841 tgcagggctt tctgatcggt cacgtccctt acgatggcga gcacctcctc ctggctgagc
      901 gcaatcatgc gtgcctcgta atggcggagc cctccaggga gagagagatc gtattcgaac
      961 tgctggagtt ccttggtatc tatcgcccgg gcgatgcgct ccatcatcag ttctgtcatc
     1021 tcctccgaaa agccggtatc cctcacgttc cttccgagta tctggtctgc aggaatggca
     1081 agtacagacc tgtccgggac atggaactcg aggtatacgc cgtcccggga gatcgtgaac
     1141 atcaggtcgg gcaccgcctc gaggagacgg gcattccgct gttcgctcct ccggagcgca
     1201 tcttctgcac gttttcggcg ggtaatatcg aggatgactc cctcgtagtg tgtgattctt
     1261 ccctcgttgt cccggcgtat gtgggtgtag tcttcgatcc agcattcttt cccgtctttt
     1321 cccagtatcc ggtactgctg gatatactcg tctatcccgt gttcgctgtt gtaagccacc
     1381 tcgccgttca cacgctcgag gtcatcgggg tggatgatat cgcgaaacgc tatcctgccg
     1441 gagaggaatt cttcggggaa atacccaaac tgggagatgt tttcgctcac cgtctcgact
     1501 ggccagccct cctcggccct ccagagaaac gcaacggcag ggctcgtgtt gatgacccgc
     1561 acctcctcct cgtggatctt gagaagttcc gcgatgcgct cctgtgccgc tctcttctcg
     1621 gtgacgtccc tcccgatgac ctggatccca tccacagccc cgctcctgat gaccggagca
     1681 aacgacatct ccatcactgc ataactgccg tcattcttcc ggacatgagc ctccaggccc
     1741 tctgtatgga ctcctcccag gttccggtcc acggcttcac tcacggactg catggattcg
     1801 gggtgaacgt aatcgaacgc agctgtgccg acgacctctt cggggcgata tccaaggatc
     1861 ctcccgatgg acggggagga gtatgtgatc gtgccggcct ggtcgaccag gagaaggaga
     1921 tctgagattc gctcggcaac gccgcggtac tgctcctcgc tctgcagaag ggcgtccctt
     1981 gcctctttta gttcggtgat gtcctggatg gcacctatca gtcttcctgt cttttctccc
     2041 tctttcactg cacgaccggt gatgtggacc cagaggcgcc ggcccttcgc actggtgagg
     2101 gggacttcga ggtctatcat ctcaccggtc gcggtacatc tctgtatcgc ctccctgatc
     2161 cgtattctgt cgggttcatc gaagaacccg agcgcctcgt caagttcgag gtggtctcgg
     2221 tcgtatactt catggaggac atacgtctcc cgcgtccacc ggatttttcc tgtcccggtg
     2281 tcgagttcga gcccccccac ccttgcaagt ctgcccacct ccgtgaggag tttctcgttc
     2341 tcaatgagcg cgttcgccgc ccgtttctgt tcggtgatgt cccggaaata ccagacacgc
     2401 ccataatatt tcccatcttt cccggttatc gatgcggaat acctgtcgaa cgtacgcccg
     2461 tccgtcagcg ctatctcctc ccggctcttc tgatcgcggt gctcatagag gtacctgacc
     2521 ctgtccagga atttctcagg ctcgaccagc aaggcaagaa cctcctgcat ggcctgttcg
     2581 tccgagtgcg tagcgagaac atccgggggg atattccaca tatcggcaaa acgctggtta
     2641 tacgagatga tctttcccca ttcatcaatg attaagatcc catcaagaga gacctcctgc
     2701 tgggtggaca ggatgatgtt tgagaattgg agcgcctctt ccgcctgcct gcggtgcagc
     2761 agctcccaca ggtggttccc gagagtggtg agggcattga tatccccctc gtcatagtct
     2821 tcgggcttgt ttgccaccgc gaggattgcg acgatcagct ccccgtcaaa tatcgggacg
     2881 ccgagatagc gggtgatggg cacgtgcccc tcgggatacc cgtgctttgc agggtgggcc
     2941 agggaataat cgttgagaat gaagggggtt ctctggcgta tggcctcccc ccagacgccg
     3001 gccgccgcga tggggaagtg catcggcttg tccgatacgg tacattcctt catcgcatcc
     3061 ttcgaccacg agtggatcgt catgaccgac tcgtcagggc ttatcagtcc tatgaaggag
     3121 tacccgctct ccgtcatatg gaggcatgct tcgagggagt acccaagcag gtcccggaac
     3181 ggcacatcct tcatccggtt gagttcaagg agattctggg tcctctgcgc ctggagcgtg
     3241 acctcttctt ctgcccgttt ttgctctgtt atgtcgacgc cgacgccgat gtaaaggggg
     3301 ggctcccctt cccggtggga gatctcgcgg gtgttccaga ggatggtctt tgtatggccc
     3361 cccctgcagg taacggtggt cgaaaaattc tcgagatgcg atttcgtcga gattatcccg
     3421 ttgatccggc gcaaaatctc gcgccggtat tcctggtcag ggtatagggc cttccatacc
     3481 tcgtttctcc ctctcacctc ctcgaaggta tacccgcaca tcagctcggc cgccgggttc
     3541 cattcgatga ctcgtccttt ttcatcgagg accatcatcc atacattcgc gttctggatg
     3601 attcgccgct gcacctgctg gctcctctcg aggtctgatc gaatcctctc caggttctcc
     3661 gcaagataga ctaccagcac cccaatcccg atgaacacga gggtccttat gacggccccc
     3721 tcaatgattt caaggtcagc gggatggaac aagacgacaa gcaccaggta caggaagctg
     3781 aggaaaacaa ttccccagag tccctgacgc cggtaccggt atgcaaggag gacgatcggg
     3841 atgtaataga ggtgcatgaa gatgatggtg atgccctgcg aaaggcagta tactgtcagg
     3901 gagatgacgg cgaccgagag caggactatc accgccttcc acgtcctctc cgccatcgcg
     3961 ggtatggggg agaacatcct gttcatgtcc ccgagccccc cgcgaaccct ttggatggac
     4021 gtcgcactgc cccggaaacg cccttcattc cttcaccttc agcaccatgg taaatgcagt
     4081 cccccgggtc ctgtccaggt cgatggtccc gtccagctgg ttcacgaggg atattaccag
     4141 tttgagcccg agcgactggg tattcctcca ttcaaggtct gccggtatac cgatgccggt
     4201 atcctccacg acaaggttga tggtcttttc ctcgcgcctc gccaggatga agagcgaccc
     4261 ggcccgtccg tccgggaatg catgcttgag tgcattcgag acgagctcgt tgatgataag
     4321 gcccagcggg atcgcagtgt tgatggggag cgtaatcttc ccaatctctg tcttcagggt
     4381 cactttccgg gaatcggtct cgtagtacgc gaagagctgg gaggtgagga accgtacata
     4441 ctcggcgaga tcgatatccg agagggtctc ggacttgtag agcttctcgt gcacgagtgc
     4501 cattgccctc acccggttct gcgtctcggc catgacctgt ctcatcttcg ggtcctctat
     4561 ctggcggagc tggaggttcg agaggctgat gatgatctgg aggttgttct tgacccggtg
     4621 atggacctcg cggaggagca gggtcttttc gttcagcgcc gcacggatct ggtcctccat
     4681 ctccctccgc cgggtgatct cctcctcgag cgcgacattc gtgtccgcca gggcatgtgt
     4741 ccgctcctct accttctgtt cgaggaccgt gttcagggag cggatctctg actccatcat
     4801 cttccggtcg gtgatatcgc ttgtaaatcc cagcagccgc tggtcatcga tcctgacggc
     4861 cttgacaagc cagtatcgga cctcgccgct cttcgtgacg tacctcgtct ctccggtcgc
     4921 ctgcccctcc tctttcactt gccggaaatg cgcgaacgcg atctgctgat caccgggggg
     4981 tatgaggtcc cgtatgttct ttccgacaag ttcggtcttc gaatacccgg tgatcgcgca
     5041 tgccgcctcg ttcaccatga ggtaattccc ctccgcatct gcaaggaata tcccgtcggg
     5101 ggcgttctcg atgtaactcc ggaacgtctc ctcgctcttt cggagcgatt cttccgactc
     5161 cttctggctg gtgatatcct gaaacaccgc aacaaaatac cctttttggg gacaggaaac
     5221 cgatatgtgg agccagatcc tgagaggggt gaagaataca tcaaatgtct cgggtctgcc
     5281 ggtcgttgca accctcccgt atatctcaaa tatttcaggt gtctgctctt ttgttccggg
     5341 gattgcctcg ctgactttct ttccggcaat atcccggagg ccggtcaggc gcccgaatgc
     5401 cgggttcact tcgagatatt cccagtccac gggcctgccc ccctcatcgt agatcatcct
     5461 gcagagcgcg aacccttcga gcatgtgatc aaagagtgcc cgaaaccgct cctcgctcct
     5521 gcggagtgcc tcttctgcct ccttcctgtc ctttaaattg gagatccagc ggtccagcgt
     5581 ggttttctcc ctgccagggt acaggaactc ctcgggaggc gtgaagtaaa aattctcata
     5641 gaggtgctcc ccgatgaatg caaacggatg cgtcatcaac acatggagca ggatgtcctg
     5701 gtcaaatcgc ctcctgtcat actggcagac cgccacgcat ctgctgccgg ggaagaaggt
     5761 attgagctta tgctcgtact cgattaaccg ctcggagccc gggagcccgc cgagcgccca
     5821 tgacatctcc cccgtgaccc tgagggcgct gtacccttcc tggagagcgt tttctgtctc
     5881 tgttgacagg agtgcgatca tcctgtcagg atcaaagacc ccgtctttca tgtatgagtc
     5941 agagactgtc aggatggaaa actgccccgt ccgaaggtac gaatcgacat cgagtccggc
     6001 atccttgagg tagcccatga tagtctctgc agtgcgggca tcaacgatgt aaaagacctt
     6061 ctcatggttc ttcaacccgt caaggagaaa ccttgtgagc acttcgcgat gctcctcgtc
     6121 cgtggaataa atgcagcaga gatggtcgcc cggcctgata tcagatatct gttttatcgg
     6181 tccatgcgca tgcgccattg aagtccgctc ctgtttttgg tctgattccg tcaaagttac
     6241 atttcccgct atgcaaagat catgacttca ttccttctca taaaggacca tcgagaacgc
     6301 ggtcccttcc ctcctgtcaa gtgcgatgct cccgttcagc tgcgacacga gcgagtttac
     6361 caggcggagc ccgagcgact tggtgtcctg ccagtcgaga ccgtcgggga tgcccacccc
     6421 agtatcgcgg tattccacgt acagggcacg atcctcccgc ctcacgctga tcgagacttc
     6481 cccccgtctc ctctccggga acccgtactt gagcgagttc gagatgagtt cgttcatgat
     6541 cagcccgaag gggatcgcgg tgttgatatc cacatggatg cctgccgcgt ctgtggagaa
     6601 ggtgatcccg cgtgttcctg ccccgtagaa ctggaaaagg ctctttccaa gatacttgag
     6661 atagtcatcg agattgatct gcgagaggtt ctcggtctgg tagagcttct cgtggacaag
     6721 cgccattgcc cgcacacggt tctggctctc ccggatcgcg gcgagggtct tttcatcggt
     6781 gatgtaacgc gactggaggt ttaagaggct gacgatgatc tggaggttgt tcttgacccg
     6841 gtggtggacc tccttgagga gagtcagttt ctcgtcaagc gacgtgcgca gcttctcttc
     6901 agcattcttt cgttcggtaa tgtcctgtcc ctgagctatc gctgcaacca cggtctttcc
     6961 gtcgcctgcc aggagcgttg ccgagtccca gagcacgata cgttcaatgc cttctcttcc
     7021 gtggatgggg atctccacgg cctcccagcg ctcaccctcc atcgcctgcc gaagatatgc
     7081 catggagcgc tcgcggctct gttcagggaa gaggacactg acaggttgtc cgatgacctc
     7141 ggctgatgga cagccggtca gccattcgaa cgcgtggtta aaccgcgtga tgcggaactc
     7201 cggatcccac acgatgatgg gggcatttgc ataatcgaag aggttattga ggtactcgtt
     7261 cgtttcgcgt aatttctctt ccgcaacttc ccgctggccg atctcggcga tgagggccag
     7321 gttcgtacgt tcgagctcgc gggtgcgctc aatgacccgc tgctcgagga cggtattcag
     7381 ggcacggatc tcggattcca tcagtttcct gttcgtgatg tcatgcgaga tcccgattat
     7441 ccgctctgtc cttttcccgg cccctttgag cgggatcagg gtcgtcgagt aataccgccg
     7501 cccgggtccg cggtccatgg tctcttcgta actgagagga gaccctgtct tgacgcactg
     7561 gcggtacatt gaggagaggt gttcgctggt ctcgccaggg aagagatctt caatgaacct
     7621 gccggatgcg gacgcgttcg tgatacccat ctcgtcctca gagacagggt tcatgtaccg
     7681 tatcctgaac cgttcatcgg cagtcacgtc gatgatgaag atgctgtcaa gggcgtgggt
     7741 gacgatctcc tcgtaccgct cctcagcctc gcggagcgcc tcctcggcca tcttgatgcc
     7801 agtgatgtcc acgaccatgc cggtgaccgt ggcaggtttt ccgtccgggt ccctgcgggt
     7861 cacccctccg ctgtcgtgga accatctcca ttccccacca gacgtcctga tccggtactc
     7921 cacgtcatac cgtggcacct tcccttcgag gtggtcccgc atggcctgca ttgtccgatc
     7981 gaggtcgtcg ggataaatga gggcgcagaa gtcctggtag gtcgcaaact gccccggggc
     8041 atagccaagc attgtcgctt tccggtcatg gaaacggacg cccccggacg gcaaatccat
     8101 ctcccaccag gcgatatttc ccatctccat ggccacgtca aggcgggact cgctctcttt
     8161 tcgtttctta tcggctcgct tctcagccga gatatcccgg gcgacgctga tgacagagag
     8221 aacctctcct gattgagaga attcagggat gatccttgat tcaaatacga cgggattttc
     8281 acccgaagga tacacaaatt cgaacgtaac cggagatccc tcctcgaaca cccggtcaag
     8341 gttctcgtcc catagcctgt tgagctcgtc gggcatcccg aggtcctggt tggtcctgcc
     8401 gaggaaggat tctgccggct ttcccgtcac ccgttcgata gcatggttaa tgtacgtatg
     8461 acggtgttcc ctgtcgaatc gcgcgatgat atcgggaatc ccttccgtga gtgtccgata
     8521 ccgtgcctcg ctcaaccgga tagcctcctc ggcctgtttc ttcccggtca catcctcgta
     8581 tattgaaacg acctccccgg atggaagcct gtatatggta ttctcccgcc atcccgaaat
     8641 ccgtccgtcc ctgtattctg agactgggtg ttgctcgggg ataccagtcc tccacacacg
     8701 ctggaacact tcaaagagcc cgaactcctt cacaccgggg aagacttcga ggacgctctt
     8761 tccaataacg tccgctcttc tgacgttctc gatccgctcc actgccgggt tgatctccct
     8821 gaagatgaag tccttgccat cctccaccac cccgtagatc gcgacacccg cggacatgtt
     8881 cgagaagagg ttcctgaacc gggcctcgct ctgcttgagc gcctcttctg cttctttttg
     8941 cgcggtgata tcgcggccga ctgactggta ctccctgact gctcctgtct cgtcgaagat
     9001 cgcccggtcg ctccactgtt gccatcgaac cctcccgtcc ggcatggtga tccggtgttc
     9061 gctggtggcg acagggtgtt cctgcgaaag tgaatcgaat acatcctgga caatctggcg
     9121 gtcttcggga aacacaacgg gcctgtatat atgcccaatc aactcctcgc gggtctttgc
     9181 gaagtaacgg gcatatgcat cattcacaaa aacgtaggtg ccatcaggtc tgaaccggca
     9241 gataaattcg gtctggtcct caaggatgtg gcggaaccgt tcctcgctct cgcggagcgc
     9301 gtcgaccatc cgcatccgct cggtgaggtc ttgaattgcc ccgaccatcc ggattgcacg
     9361 tccctccggg tcgcgtatta cgaaaccgcg atcgaacacg tatgcgtagg ttccgtcagc
     9421 cttccgaaac cggtattcgt ctgaccattt gttctcccca ccgtctttgg ctgcgtggat
     9481 gccatcgata atccgttccc ggtcatcagg atgaatgcgg gtataccacg atgcgacagt
     9541 cggctcaatt tcctccgggc ggtagccgaa cactgattgg atcccctcgt tccaccactg
     9601 gctgtctgtg agaaggtccc agtcccagac ggtatcattg gtggctttcg agacgatctt
     9661 gaacctttca ttcgattccc ggatctcttc ttcagccctt ttctggttgg tgatatccct
     9721 gaccacggat accgatccga tgatcgcacc ggtcgtatcc cgcaccggtg tggagctgat
     9781 ctggcggtac cggaattccc cggtcacggg tgtccggatg atctcctcca tgttcctgat
     9841 cacttcgcct gagagggcac gcaaaggcgg ggcttcctcg acagggcggg gggtgccatc
     9901 cgggcggagt acttcgaggc tccttgcaag atcttctacg atgggggtat cgccctcgcg
     9961 aagttgaaat tcttccaggg cctgggggtt ggcgagggtg aaccggtgat cggtgtcggt
    10021 gaaccagacc tcgtcagtga tgctgttgag gagggtcgag agccgctctt tttcgtttct
    10081 cgcggactcg aggagctcac tgaccctgat ttctgcctct tttttatccg tgatgtcgac
    10141 gatagtccca atcaggcgga ttggcctgcc atcatcacat tccacctgtg cgattgtttt
    10201 tacccatttt tttacccctg tcccgctcac gaatctcatt tcgagattgt atggaattcc
    10261 ctcctcaacc gccctgtgga acgcgtcctc aagaatcgag cgatcttcgg gggaataaaa
    10321 tgccaggtcc tcctggatat tattggggtc aaaatcaacc ggcacctcgt aaatacgata
    10381 gacctcatct gtccacgtaa ttgtaccgga caggatatca tactcccagc cgcccacatg
    10441 cgcatactgc tgggtctggt tcagcagctt ctcgctcttg atgagagccc tctcgctctc
    10501 tttgcgttca gtgatatccc gtatgacctc gactgcgcct gccaggttcc catgcagatc
    10561 gtagagcggg gatgcggtgc cccagaggtg ggcacctctt ccctcaaaga tatcagggat
    10621 aaaagtctca gatgtgatct tatccccgtc ccgctggatg tacaggtatt tctgctcgat
    10681 attatcccgg cttttcagta ccaggtcgat caggatcggc cgccgctcac cgtagaacgg
    10741 gatgctgtat tcgtaatcgc ctctcccgat tatctggtcc gaggggacgc cggtcatcgc
    10801 ctcgattgcc ctgttccatg caatgactct tccctccgcg tcgattgcaa aggtcgcatc
    10861 ggggaggaaa tcgaggatat ctg
//
LOCUS       DAOQYR010000074        10869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_6493_length_10869_cov_8.244128, whole genome shotgun sequence.
ACCESSION   DAOQYR010000074 DAOQYR010000000
VERSION     DAOQYR010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 10869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10869
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6493_length_10869_cov_8.244128"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..997)
                     /gene="mtxX"
                     /locus_tag="PKM87_08730"
     CDS             complement(185..997)
                     /gene="mtxX"
                     /locus_tag="PKM87_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker protein Mmp4/MtxX"
                     /protein_id="HNQ30188.1"
                     /translation="MPSLKIGIGVLGEHEKVVASVRGSRCPSRAVVYCRPGELVQADT
                     IEIVEDPDPGEALVRDLFQGKIHAAVRGTLPSNSTLSALKRVAGVECLERAALLETGD
                     GRKFLLAPVGVDEGWTVQEKVSLARKSERLARTLGLREGVSILSGGRLGDAGRHPRVD
                     ESMAQAELVARLSGAHHSEILIENAVNEAGVIIAPDGISGNLIFRTLTFLGSGHGHGA
                     PVLNIDRIFVDTSRASPDFINALHLADFLSNRQKVNLNNPFQEKGHNIVKKV"
     gene            complement(985..1998)
                     /locus_tag="PKM87_08735"
     CDS             complement(985..1998)
                     /locus_tag="PKM87_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 2 protein"
                     /protein_id="HNQ30189.1"
                     /translation="MELNGVSTAKIASVVREYEGVRRKHAIGEMVKAVRIDSPGVLAS
                     FGEDAAVIEHGDEVLLLAADGIWSRLMEADPYWAGYCAVLVNIHDIAAMGGRPVAMVD
                     VFSISDSGIQKQVIAGMHDASIQFGVPIVGGHLHPDTPYSVIDVAILGTAQKDSVIYS
                     HTAREGDCVIVAVDLNGRVHPSCILNWDSVTMKTAAEVRSQVKVLEGLGRDRLVTAGK
                     DISNPGVIGTLGMLLEVSGKGATVALDRLPMPDLAEHHMTFEQWVRIYPGMGFVLTAP
                     HRNVPEISRRFEEVGMTACPVGTVTHDRLLRLVLGNDTSQVFNFDKNGIMRLFSDDGS
                     CPH"
     gene            2081..3481
                     /locus_tag="PKM87_08740"
     CDS             2081..3481
                     /locus_tag="PKM87_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C large subunit"
                     /protein_id="HNQ30190.1"
                     /translation="MDWAEKYRPAHLDEVVGNSTALRQMLEWARTWSRDRKPLILYGK
                     PGTGKTSSAHALASDMGWEVIELNASDQRTKSAIERVAGSGSTTASLTGAQRKLIIID
                     EADNLHGTADRGGARAIVDLIRGSRQPILLIANDLYAIPGEIRSRCEGVQFKALQARS
                     IAPRLRYICAAEKVSCSEQALQRISEAARGDMRAAVNMLYASAAGKEKLNEEILPSRK
                     DERSSVFDLVIALYGRTPADSLLGIAYEVDETPDTLIQWIESNLSGIGEIDDLDAAYS
                     SLSRADEYLGYTYRQQYHTLWRYATALMVLGVGRVAGGRGLHARISAPERWRRMSSYQ
                     KQKAVRLSMLRKVSSALHMPQRTFLETYMSPVAMVIDRDPLTYAREFLLDTDELDLFI
                     HDRGRAASVARTLAAEEKEREIKREKESSARKRRDEGGSGRQKEIPLKRDDTTPSVDT
                     PGKASQKDQKTLFEGF"
     gene            complement(3454..3876)
                     /locus_tag="PKM87_08745"
     CDS             complement(3454..3876)
                     /locus_tag="PKM87_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0146 family protein"
                     /protein_id="HNQ30191.1"
                     /translation="MAGLKRIEHCIASYIAKHYRCAAEIGVGENPEVALILNNRGVKV
                     FCTDIRAVGGVPGIRFVQDDIFSPEVCLYRDIDLIYSIRPHEEMMPALLSLARNVDSD
                     LLVYHLGFEGFLDGGELIDCGVVLHRYSRRQNPSKRVF"
     gene            3895..4416
                     /locus_tag="PKM87_08750"
     CDS             3895..4416
                     /locus_tag="PKM87_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M54"
                     /protein_id="HNQ30192.1"
                     /translation="MELLIFWDARAPAGIQSAFSREIERLLPLKTNICESPFVVTGYI
                     PSRRQTNATSLLDSLDLYRKRSGVSGLVLLVVSEDLCRPGDEYLFGLSRRSTGNAVVS
                     TARLGNEFYGYPPDDGDLIDRLSKESAHEAGHLLGLAHCADPECIMHNPLLLEDITRQ
                     KRAFCAACRAQIP"
     gene            4530..5039
                     /locus_tag="PKM87_08755"
     CDS             4530..5039
                     /locus_tag="PKM87_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNQ30193.1"
                     /translation="MLERDTHPADEAAGNQQKSAGKDIDIIAVSDLMTRDFEKILPDT
                     PIDHCITHFSQRGCRDLVVVDHEGRFLGIVTPVDLLTHLSPAIGVRSRKKSGCIECII
                     VGNASTAEDVMARKHITVRGDTPVSEALRLLEKYHHPDLVVVDQEGVAMGVVEICTII
                     AHLKVTGKV"
     gene            complement(5036..6187)
                     /locus_tag="PKM87_08760"
     CDS             complement(5036..6187)
                     /locus_tag="PKM87_08760"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNQ30194.1"
                     /translation="MSPLAVILLLLVTAKGLGELLERVGFPSLIGEIAAGAILGPAVL
                     GLIGPGEPIEVFADIGLIVLLFMSGAQLNMRTFANSGKAGAIVAVAGVAIPLFAGIVL
                     GIASGFDTIHSLFIGIALSITSIGISVRTLIDLRQLKSDIGLLVVSSAVIDDVIGIMM
                     LGILAALVTGEGYGAGVLTTIVLAILFLVVVLTAGKRVLVWVFQESHKAHTHEMPYSV
                     ALICGLLCALAANEAGLHFAIGAFIAGLILGDSIRSDRSLYDSLSDLAFGFFVTLFFA
                     YIGLLFPPSLAGVPLVFFAVLVAVSFASKIVGGFIGSFRTLQNPRKALVVGFGLVPRG
                     EVALVVAKVSLTAGIISISLFSAVTLMVVITVFAAPFLMVRGFTWLRGD"
     gene            complement(6333..6644)
                     /gene="rpl12p"
                     /locus_tag="PKM87_08765"
     CDS             complement(6333..6644)
                     /gene="rpl12p"
                     /locus_tag="PKM87_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein P1"
                     /protein_id="HNQ30195.1"
                     /translation="MEYIYAALLLHKAGKEVKEENVKAVLTAVGIAVDDARVKAMVAA
                     LEGINIEDAISKAAAAPVAVAAAAPAAAAAAPAAAEEPTKEEKKEEEESGMAGLGALF
                     G"
     gene            complement(6666..7520)
                     /locus_tag="PKM87_08770"
     CDS             complement(6666..7520)
                     /locus_tag="PKM87_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNQ30196.1"
                     /translation="MALYTHHLPAWKRREVEEIKADAGKYALVGLVDMYGIPASQLQQ
                     IRRNLKSTAKIKMTRNTLIRHAFDEMGGPVAALSGHLSGQSALIFTDENPFRLFKTLE
                     KTKTKMAAKPGETAPEDIVVEKGPTSFKPGPIVGELQQAGIPAAIEAGKVKIRETRTV
                     VKKGEVINPKLADVLAKLDIKPMDVGLILQAALFEGDVYEPSVLSIDEDLIYSQLTLA
                     ASQAFNLSVNAVIFTRETAVPLVEKAAREARGLAIEADVYAKDVIDAIIGKAYRESAA
                     LQSMVGGK"
     gene            complement(7521..8165)
                     /locus_tag="PKM87_08775"
     CDS             complement(7521..8165)
                     /locus_tag="PKM87_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNQ30197.1"
                     /translation="MVERAKIVDAVKAAIEKAPKRKFSESVDITVNLKNIDMAQPKNR
                     IDETILLPHGTGRKVGIAVIGKGDITTQAREAGVDLVIGPEEIERLGGAPREARSVAS
                     AYRFFLAETSVMPQVGRYLGPRLGPRGRMPTPIPSGTDIKPMVERLRNSVKIRTKDKK
                     TFHVKVGSTGMPPEQIAENIDAVMRRVESVLEQGSMNIRSVYVKTTMGPAERLV"
     gene            complement(8218..8694)
                     /locus_tag="PKM87_08780"
     CDS             complement(8218..8694)
                     /locus_tag="PKM87_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNQ30198.1"
                     /translation="MAEVVEVLVPGGKATAGPPLGPALGPLGINVKAVVDEINAKTGS
                     FNGMQVPVKVEVDDKKNFTVSVGIPPTTALIKKEVNVEKGSPEPNIKVVGDLPIEAAV
                     RIARMKQDDMLSYDLKHGVKEVIGTCVSMGITVDGKKPKEALSAVDAGTYDKLLVE"
     gene            complement(8797..9270)
                     /locus_tag="PKM87_08785"
     CDS             complement(8797..9270)
                     /locus_tag="PKM87_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor Spt5"
                     /protein_id="HNQ30199.1"
                     /translation="MEEFPNRIFAIKTTSKQERTVADNIKKAIETGATDIQVVAIIVP
                     DELKGYVLVETPESLPRIEQLAEKVAHARTVVRGETTLSEVAHFLVPKPVVSGISEGT
                     IVELIAGPFKGEKAVVKRIDTGKEEITVELYESVLPIPITVRGDNVRVVDKGDGN"
     gene            complement(9276..9470)
                     /locus_tag="PKM87_08790"
     CDS             complement(9276..9470)
                     /locus_tag="PKM87_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase SEC61 complex subunit gamma"
                     /protein_id="HNQ30200.1"
                     /translation="MEIAKPDLKFNINEELFRKYWRILKLARTPKREEFQKIALVAAA
                     GVLIVGLIGFVIYELMLFLP"
     gene            complement(9476..10567)
                     /gene="ftsZ"
                     /locus_tag="PKM87_08795"
     CDS             complement(9476..10567)
                     /gene="ftsZ"
                     /locus_tag="PKM87_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNQ30201.1"
                     /translation="MRSIVEEALTRVREDSPDISKNNEDLDQILKELRTEIAVIGCGG
                     SGSNTITRMKEEGIHGARLVAINTDAQHLSRTVADHRILIGRQRTRGLGAGSIPQIGE
                     EAALENEEEIKRAVSGCDMVFITVGLGGGTGTGAAPIVAKSAREEGALTIGVVTLPFT
                     AEGVIRMENAEAGLERLRDVADTVIVVPNDRLLEVVPKLPLYAAFKVADEVLMRAVKG
                     ITELITLPGLVNLDFADVRTVMERGGVAMIGVGESESEEKAADSVKKAIRSPLLDVDI
                     SGATAALVNVIGGPDMTMEEAEGVLQEVYNRIAPDARIIWGAQVDPNMQNKMRTMLVV
                     TGVRSPQIYGRNEKITPKVQKQFEIDFLK"
     gene            complement(10564..>10869)
                     /locus_tag="PKM87_08800"
     CDS             complement(10564..>10869)
                     /locus_tag="PKM87_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HNQ30202.1"
                     /translation="VWPSFITIGENSGNVLHDLISLCVNIIRRGEITFVAGDHLTICR
                     RRFDPDRARDLGIPPGPLYGQLMNGCPVRIGDREITPEMVQTRSEKRIHIPGLEKFL"
BASE COUNT         2284 a         3196 c         2992 g         2397 t
ORIGIN      
        1 ccattcttct tggcaattct cacaactgca gcaataggta aatctgccat tgtatattac
       61 ctcaaatcaa aaaatgtatc aggaaatatt taaacatttc gtgattttcg tgcaatttgt
      121 cccccattat ctttttgcgc ggttctccgg aacgattaca agcagagcat gcagaataag
      181 agtatcagac ttttttcact atattatgac ctttttcttg aaaaggattg tttaaattga
      241 ctttttgcct attggacaaa aaatctgcca gatgaagcgc gttgatgaaa tctggcgagg
      301 cgcgcgaagt atctacaaag attctatcga tatttaggac tggtgcgcca tgtccgtgac
      361 ccgatccgag aaaggtgagc gtacgaaaga tcaaattccc ggaaatgccg tcaggagcga
      421 tgatgactcc ggcctcgttt actgcattct cgatgaggat ttcggagtga tgagcccccg
      481 aaaggcgtgc gaccagttct gcctgtgcca tactctcgtc gactcgtggg tgtctcccgg
      541 cgtccccgag ccttcctccc gaaaggatgg agacaccctc gcggaggccg agggtgcgag
      601 ccagtctttc agacttgcgg gcaagcgata ccttctcctg gacagtccac ccctcatcta
      661 ctcccaccgg ggccaggaga aacttcctcc catcacccgt ctcgagaagc gccgctctct
      721 cgaggcattc cacccccgcc actctcttga gtgcagagag cgtggaattg gaggggagcg
      781 tccctctcac ggcagcgtgg attttaccct ggaagagatc gcgtacaagg gcttcgccgg
      841 gatcggggtc ttcaacaatc tcgatggtat ctgcctggac taattctccg gggcggcagt
      901 atactacagc ccgtgacggg catcgtgaac cacgcacact ggcaacgact ttttcgtgct
      961 ccccgagaac cccaatccca atctttaatg agggcatgag ccgtcatctg aaaaaaggcg
     1021 catgatgccg tttttgtcga aattgaatac ctgagaggtg tcgttcccga gcaccaacct
     1081 gagcagcctg tcatgggtta ccgtcccgac aggacaggcg gtcatcccga cttcctcaaa
     1141 gcgcctggag atctccggga cattccggtg aggggcagtg aggacaaaac ccattcccgg
     1201 gtatatccgt acccactgct caaaggtcat gtggtgttct gcgaggtcgg gcatcgggag
     1261 cctgtcaagg gcaacggtag cccctttccc gctcacttcg agaagcatcc cgagtgtccc
     1321 gattaccccg ggattgctga tgtccttccc ggcggtgacc agccggtcgc gaccgagccc
     1381 ctccagcacc ttcacctgtg accgcacctc ggcagcagtc ttcatcgtca ccgagtccca
     1441 gttgaggata cacgaggggt ggacccttcc attgaggtcg actgccacga tcacgcagtc
     1501 accttccctc gcggtatgcg aataaatcac ggagtctttc tgcgccgtcc cgaggattgc
     1561 cacatcgatc acgctgtatg gtgtatccgg atggagatgg cctccgacga taggaacccc
     1621 aaactgtatt gaagcgtcgt gcatcccggc gatcacctgc ttctgtatcc ctgaatcaga
     1681 gattgagaag acatcaacca tggcgacggg ccggcctccc atcgcggcaa tgtcgtggat
     1741 attgacgagc actgcgcagt aaccggccca gtaaggatcc gcttccatca accggctcca
     1801 gatgccgtcg gcagcaagga gaaggacttc gtcgccgtgc tcgatgaccg cggcatcctc
     1861 gccaaacgat gccagcacgc caggagaatc gatccggacg gctttcacca tctcgccaat
     1921 ggcgtgcttg cgcctgaccc cttcgtactc ccttaccacc gatgcaattt ttgccgtaga
     1981 tacgccgttc aattccatag agacaacaga gccggtttgt tatatctcat tggagcatca
     2041 tcagtgataa tttatttgat acagggtcga atcaagtggc atggactggg cggaaaaata
     2101 ccggcctgca cacctcgacg aggtggtggg aaacagcacc gcgttgcgcc agatgctgga
     2161 atgggccagg acgtggagcc gggacagaaa gccgctcatc ctctacggca agccgggtac
     2221 cggcaagacc tccagtgcgc acgcgcttgc atccgacatg ggctgggagg taatcgagct
     2281 caatgcgagc gatcagcgga ctaaatctgc cattgaacgc gtcgccggga gcggaagcac
     2341 cactgcgagc ctcaccggcg cacagagaaa gctgatcatc atcgatgagg cggataacct
     2401 tcatgggacc gcagacaggg gaggtgcccg tgcgattgtt gatcttatca ggggatcccg
     2461 ccagccgatt ctcctcatcg ctaatgacct ctacgcaata ccgggcgaga tcaggagccg
     2521 gtgcgagggc gtccagttca aagcgctcca ggcgcgttcc attgccccgc gtctccgtta
     2581 catatgtgca gcggagaaag tttcgtgcag cgagcaggcc ctccagcgta tctctgaggc
     2641 ggcccgtggc gacatgaggg ctgcagtaaa catgctctat gcgtccgctg ccggaaaaga
     2701 gaagctcaac gaggagatcc tcccctccag gaaagacgaa cggtcatccg tgtttgatct
     2761 cgtcatcgcg ctttatggca ggacacccgc cgattccctt cttggtatcg catacgaggt
     2821 ggacgagacc cccgacaccc tcatccagtg gatcgagtcg aacctttccg ggattggtga
     2881 gatcgatgat cttgacgcgg catactcctc cctttcccgg gctgacgaat acctcggcta
     2941 tacttaccgc cagcagtacc acacgctctg gaggtatgcg actgccctca tggtgctcgg
     3001 tgtcggcaga gtggccggag gcagggggct ccatgcccgg atatccgcac ctgagcgttg
     3061 gagaaggatg tcgtcgtacc agaaacagaa ggcggtgagg ctgtcaatgc tccggaaggt
     3121 ctcatcagct ctgcatatgc cgcaacgaac gtttcttgag acctacatgt ccccggttgc
     3181 gatggtcatc gaccgtgatc ctctcaccta tgcacgggag ttcctgctcg ataccgacga
     3241 gcttgacctt ttcatccatg accggggacg tgccgcctcg gtcgcaagga cgcttgctgc
     3301 agaagagaag gagcgggaga taaagagaga gaaggagtca tcggccagaa agcgacggga
     3361 tgaggggggt tccggcaggc agaaggagat ccccctcaaa agagacgata cgacaccatc
     3421 ggtcgatacg cccgggaagg catcgcagaa ggatcagaag accctctttg aagggttctg
     3481 acgacgggag taacggtgga ggacaacgcc gcagtctatc agctcgccac cgtccaggaa
     3541 cccttcaaat ccaaggtggt acacgagcag atcggagtcg acattgcgtg cgaggctgag
     3601 cagggcgggc atcatctcct cgtgcggccg gatggagtag ataagatcga tatcccggta
     3661 aagacatact tctggggaga agatatcatc ctgtacaaac cggatcccgg gaacaccccc
     3721 aaccgccctg atatctgtgc aaaacacttt tactccccga ttgttcagga tcagggcaac
     3781 ttccgggttc tccccgaccc ctatttccgc cgcgcagcgg taatgttttg cgatgtagga
     3841 cgcgatgcaa tgctcaatac gtttaagccc cgccatatcc acttatattg agagatggaa
     3901 ctgcttattt tttgggatgc ccgggctcca gccggtatcc agtccgcatt ttcgagggag
     3961 attgaacgcc tcctccccct caagacgaat atatgcgaaa gccccttcgt ggtaaccggc
     4021 tacatccctt caaggcgcca gaccaatgcc acatcgctcc ttgactctct tgacctctac
     4081 aggaagcgct cgggtgtcag cggcctggtc ctcctcgtgg tatcagagga cctctgcagg
     4141 ccaggggacg aatacctctt cggcctctcc cggcggtcga cggggaacgc cgtggtatcc
     4201 actgcccggc tgggaaacga gttctacggg tatccgcctg acgacgggga tctcattgac
     4261 cgcctgtcaa aggagtccgc ccacgaggca ggccacctcc tgggacttgc gcattgcgca
     4321 gacccggaat gcatcatgca caaccctctt ctcctggagg acatcacaag gcagaaacgg
     4381 gcattctgtg cagcatgccg ggcacagatc ccgtaacccc aactggaggg ggataggcct
     4441 gattccttgc ttttattttt tgggagccac tccctttata tgaacgcagg caccatcaac
     4501 tcccgaagcg aaggaattaa ggacctttta tgttggagag ggatacgcat ccggctgatg
     4561 aggctgcggg taaccagcag aaaagtgcag gaaaagacat cgatatcata gccgtctcag
     4621 atctcatgac ccgcgacttc gaaaagatcc ttcccgatac cccgatcgac cactgcatca
     4681 cgcatttctc gcaaaggggc tgcagggacc tcgtggttgt cgaccacgaa ggccgttttc
     4741 tggggatcgt cacccccgtt gatctcctta cccatctcag tcccgccata ggtgtccgga
     4801 gcaggaagaa gtcagggtgc atcgagtgca ttatcgtggg caatgcatcg actgcagagg
     4861 acgtcatggc gaggaagcat atcactgttc gcggggatac tccggtctcc gaggcgctca
     4921 ggctgcttga gaagtatcat caccctgacc tcgtggtggt cgatcaggag ggagttgcga
     4981 tgggggtcgt cgagatctgc acaatcatcg cacatctcaa ggtgaccggg aaggtttaat
     5041 ctccacggag ccaggtaaaa ccccggacca tcaggaatgg cgcggcaaag accgtgatga
     5101 ccaccataag ggttacagcc gagaagaggg aaatagagat aatccccgct gtcagcgaga
     5161 ctttggcgac tacgagcgcc acctcacccc tgggaacaag cccgaatcct actacgagtg
     5221 ctttcctggg gttttggagg gttctaaaag acccgatgaa tcctccgact atcttgcttg
     5281 caaatgacac tgccacgagc accgcaaaga ataccagtgg aactcctgca agggacggcg
     5341 gaaagaggag cccaatatat gcaaagaaga gcgtgacaaa gaagccgaac gcaagatcgg
     5401 agaggctgtc gtaaagcgac cggtcgctcc ggatgctatc gccgaggatg agccctgcga
     5461 tgaacgcgcc gatcgcgaaa tggagccctg cctcgttcgc tgcaagtgca cagaggagac
     5521 cgcatatgag ggcgacggaa tagggcatct cgtgggtatg cgccttgtgg gattcctgga
     5581 atacccacac gagcacccgc tttcctgcag tcagcaccac caccaggaag agtatggcga
     5641 gaacaatcgt ggtgagaaca cccgcaccat agccttctcc tgtgacaagt gctgcgagta
     5701 tcccgagcat catgatgcca atcacgtcgt cgattaccgc ggaactgacc accaggagcc
     5761 cgatatcgga tttgagctgc cgcaggtcta tgagcgtgcg gaccgagatc ccaatggaag
     5821 tgatcgagag cgcaatgcca atgaagaggg aatggatcgt gtcgaaccct gatgcgatcc
     5881 cgaggacaat tccggcgaag agggggattg cgacacccgc gaccgcaact attgcaccgg
     5941 cctttccaga attcgcaaat gtcctcatgt tcaattgagc accgctcatg aagaggagga
     6001 caataagccc gatatccgca aagacctcga ttggctcccc gggcccgatg agcccgagca
     6061 ccgcggggcc caggattgca cctgcggcaa tctccccgat taatgaggga aaacccaccc
     6121 tttcaagcag ctccccgagg ccttttgcag tcacaaggag aaggaggatg acagcgagcg
     6181 ggctcatcag agtgatgata tgaggatatc ggatatcaat aaggcggttg gtgaaaggaa
     6241 ccgccgtggc tggcccggtc gtgattcaga caagaaatgc aggactgttc ctgcgtttgg
     6301 gtcctgtgcg tgaactggaa aaaaaggatg gttcagccga acagggctcc aagccctgcc
     6361 atgccgctct cttcctcttc cttcttctct tcctttgtcg gctcttcggc agcggcaggt
     6421 gcggcagcgg ctgcagcagg tgcagctgcg gctactgcga caggtgcggc tgcagccttg
     6481 ctgatagcat cctcgatgtt gatcccctcg agggctgcga ccatggcctt cacccgggcg
     6541 tcgtctacag cgataccgac agcagtcagg actgccttta cgttctcttc cttgacctct
     6601 tttcctgcct tgtgcaggag gagggctgca tagatatact ccatctcaat tacctctgat
     6661 ttctctcatt tcccgcctac catgctctgg agggcggcac tttctcgata ggcttttcca
     6721 ataatcgcat caatgacgtc ttttgcatag acatcggctt cgattgccag tccacgggcc
     6781 tcccgtgcag ccttctccac cagcgggacc gcagtctccc tggtgaatat caccgcgttc
     6841 accgagaggt tgaaggcctg cgacgctgcc agtgtcagct ggctatatat caggtcttca
     6901 tcgatggaca gcaccgacgg ttcgtacacg tcaccctcga agagagcagc ctgcaggatc
     6961 agccccacgt ccatgggttt gatgtccagt tttgcaagga catctgcaag cttggggttg
     7021 atgacctctc ccttctttac gacggtccgc gtctcccgta tcttgacttt cccggcctcg
     7081 attgcagcgg gaatgcctgc ctgctgcagt tcgccgacga ttggacccgg cttgaaactc
     7141 gttgggcctt tctctaccac gatgtcttct ggagcagtct caccaggctt tgcagccatc
     7201 ttggtcttgg tcttctccag ggtcttgaaa agcctgaacg ggttttcatc ggtgaagatg
     7261 agcgccgact gccccgagag atgaccggaa agtgccgcga ccggcccgcc catctcgtcg
     7321 aatgcgtggc ggataagcgt attccgggtc atcttgatct tggcggtgga cttcaggttc
     7381 cggcggatct gctggagctg gctggcgggg atgccgtaca tatcaacaag gcctaccagg
     7441 gcgtacttcc cggcatctgc cttgatctcc tccacctctc tcctcttcca cgcggggagg
     7501 tgatgagtat acagggccat ctaaaccagc ctctctgcgg gccccatggt ggtcttgaca
     7561 tagaccgacc ggatattcat ggagccctgt tccagcacgg attcgaccct ccgcatgacc
     7621 gcatcgatat tctcggcgat ctgctcgggg ggcatcccgg tggatcccac cttgacgtgg
     7681 aacgtcttct tgtcctttgt gcgtatcttc accgaattgc ggagacgttc caccattggc
     7741 ttgatgtcgg tcccggaagg aatcggcgtc ggcattctcc cgcgtggccc caggcggggt
     7801 ccgagatatc gtcccacctg cggcatgaca ctggtctcgg ccaaaaagaa gcgatatgcg
     7861 ctggctacgc tgcgggcttc acgtggagcc ccgcccagcc gctcgatctc ttcgggtccg
     7921 atcaccaggt ccacgcccgc ttccctggcc tgggtggtaa tgtcaccctt gccaatgaca
     7981 gcgatgccga ccttcctgcc ggtaccatgg gggaggagta tcgtctcatc aatacgattt
     8041 ttgggctgcg ccatgtcaat attcttgaga ttgacggtaa tatctacgct ctcagagaat
     8101 ttccttttag gcgctttttc gatggccgcc ttgacggcat ctacaatttt tgccctttca
     8161 accattcatt tcctccatag tgcgcgacca tactgatctt ctatggttct ctctcgtcta
     8221 ttccacgagg agcttgtcgt aggtgccagc gtcgacggcg gagagtgcct ctttgggctt
     8281 ctttccatcg acggtgatac ccatactcac gcaggtgccg atgacctcct tgaccccatg
     8341 cttgaggtca taggagagca tgtcgtcctg cttcatacgg gcaatcctga cagcggcctc
     8401 gataggcagg tcaccaacaa ccttgatgtt gggttccgga gatcccttct cgacattgac
     8461 ctccttcttg atgagggcgg tggtgggcgg gatacccacg cttaccgtga agttcttctt
     8521 gtcgtcgacc tcgaccttca ccgggacctg catgccgttg aagctgccgg tctttgcgtt
     8581 gatctcgtcg acaactgcct tcacattgat gccgagcggc ccgagggccg gccccagggg
     8641 tggacctgcg gttgccttgc ctccaggtac taatacctcg accacttctg ccatgtctgt
     8701 accaccatgc cgtcccggat gggaccaagc gtgggtaaga aaggttgacc tccgcagact
     8761 taaaggtata ccaggagggg gaggaaattt tggggcttaa ttcccatctc ccttgtccac
     8821 cacgcggaca ttgtcgcccc tgactgtgat gggaatgggg agcacgcttt cgtagagctc
     8881 gacggtgatc tcctcttttc cagtatctat ccgcttgacg acggcctttt ctcccttgaa
     8941 tggtccggcg ataagctcga cgatggtccc ctcggagatg ccgctgacaa cgggttttgg
     9001 caccaggaag tgtgcgacct cggagagggt ggtctctccc ctgaccaccg tgcgcgcgtg
     9061 tgcgaccttc tccgcgagct gctcgatacg gggaaggctc tcgggcgtct ccacgaggac
     9121 atagcctttc aactcgtccg ggacgatgat tgcgaccacc tggatatcgg tggctccagt
     9181 ctcaattgcc ttcttgatat tgtcggcaac tgtcctttcc tgcttggagg tcgtctttat
     9241 ggcaaaaatc cggttgggga actcttccat attcatcagg ggagaaagag catcagctcg
     9301 tagatcacaa atccgatgag gcccacgata aggacccctg ccgcggcgac gagcgcgatc
     9361 ttctggaact cctcgcgctt cggggtcctc gcaagcttga ggatcctcca gtacttcctg
     9421 aagagctcct cgttgatatt gaatttcaaa tccggcttgg caatttccat agcactcact
     9481 tcaaaaagtc aatctcgaat tgcttctgaa cttttggggt aattttttca ttcctaccat
     9541 atatttgtgg cgaccgcaca cccgtcacga ccagcatcgt ccgcatttta ttctgcatat
     9601 tggggtccac ctgcgcaccc catatgatcc gcgcatccgg ggcgatgcgg ttgtagacct
     9661 cctggagcac cccctccgcc tcctccattg tcatgtccgg gcccccgatg acgttgacga
     9721 gggcggcggt tgcccccgag atatccacgt caagcagcgg cgagcgaatg gcctttttca
     9781 cagagtccgc ggccttctcc tcgctctcgc tttctcccac gcctatcatg gcgacgccgc
     9841 cccgctccat gacagtgcgg acatctgcaa agtccaggtt cacgagtccc gggagggtga
     9901 tgagttccgt gatccccttg actgccctca tcagcacctc gtcggcgacc ttgaacgcgg
     9961 catacagggg aagctttggg accacctcaa ggagccggtc gtttgggacc acgatgacgg
    10021 tatctgccac gtcccttaag cgctcgagcc ctgcctcagc attctccata cggatcacac
    10081 cttctgcggt gaaggggagc gtgaccacgc cgatggtgag ggctccttcc tctcttgcgg
    10141 acttggcaac gatgggagca gcgcctgtgc cggtcccccc gcccaggccc accgtgatga
    10201 agaccatgtc acagccgctg accgcacgct tgatctcctc ctcgttctca agtgccgctt
    10261 cctcgccgat ctgagggatg gagcctgcac cgaggccccg tgtgcgctgc ctgccaatga
    10321 ggatgcggtg gtccgctacc gtgcgggaga ggtgctgggc atcggtgttg atggcgacga
    10381 gccgtgcgcc gtggatcccc tcctccttca tgcgggtgat ggtgttggaa ccgctcccgc
    10441 cgcagccgat cacggcgatc tctgtccgca actccttgag gatctggtcg agatcctcgt
    10501 tattcttcga gatatccggt gagtcctccc tcaccctggt cagcgcttct tcgactatag
    10561 acctcatagg aatttctcca gtcccgggat gtggattcgc ttttcgctgc gggtctggac
    10621 catctcgggg gtgatctccc tgtccccgat ccgtacaggg cagccgttca tcagctgtcc
    10681 gtacagcggg ccgggaggta ttcccaggtc gcgtgcccgg tcggggtcga acctgcgcct
    10741 gcagatggtc aggtggtcac ccgcaacgaa cgtaatttcc cctctacgta tgatgtttac
    10801 gcataagctt attaaatcat gcagtacatt cccgctattt tcaccgatgg tgatgaaaga
    10861 aggccatac
//
LOCUS       DAOQYR010000075        10855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_6506_length_10855_cov_7.770926, whole genome shotgun sequence.
ACCESSION   DAOQYR010000075 DAOQYR010000000
VERSION     DAOQYR010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 10855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10855
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6506_length_10855_cov_7.770926"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PKM87_08805"
     CDS             complement(<1..617)
                     /locus_tag="PKM87_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30203.1"
                     /translation="MAAGIREALSVLCAPGQVVEVRAITDEGMASGYFDDLDALAAAV
                     EPLDASGTQGIYVTLNEVNQALLSRRANRIKVRLGKKDATTADGDILRRRWFPVDIDP
                     VRPSGVSSTDAEHQAAIVKAAEVAAYLGRERGWPAPVLADSGNGAHLLYRIDLPNDDA
                     SRDLVKRCLEVLDTIFSDSDCSVDCANHNAARIWKLYGTVSRKGD"
     gene            complement(622..867)
                     /locus_tag="PKM87_08810"
     CDS             complement(622..867)
                     /locus_tag="PKM87_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30204.1"
                     /translation="MTLELIPVSDERLLLERMRGLEWSIDRLHRDLAACEKERSEILD
                     HCLRHEIFEAEGFRLVKRQGVPGESQDAWRVERVSAE"
     gene            complement(857..1207)
                     /locus_tag="PKM87_08815"
     CDS             complement(857..1207)
                     /locus_tag="PKM87_08815"
                     /inference="COORDINATES: protein motif:HMM:NF013987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30205.1"
                     /translation="MQSLERTRGRRVEYLARDRLRSRGFCVIRSAGSRAPVDLVAGRH
                     REVLFVQARRTRRPLADARAVVSRYPGEITRLQAMLRRIELPVQLWLYTDAGGWRIFT
                     IHVGGIAEVSGDDA"
     gene            complement(1194..1721)
                     /locus_tag="PKM87_08820"
     CDS             complement(1194..1721)
                     /locus_tag="PKM87_08820"
                     /inference="COORDINATES: protein motif:HMM:NF017666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HNQ30206.1"
                     /translation="MTVPSALLSHRTDEHLTPRYILDAVLECLGGIDLDPSSNSRCCP
                     NVPAARHFTRGDDGLSQPWDGRVFLNPPFGRDVDRWFQKLAAEYCAGRTVAAVVLWKS
                     ATETRAWRILTGSANVICFPTRRIDFEGEGPRTGSTFSPALFYVGREHTRFARAFAPV
                     GEIWVVPRRLVHAEP"
     gene            complement(1921..2061)
                     /locus_tag="PKM87_08825"
     CDS             complement(1921..2061)
                     /locus_tag="PKM87_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30207.1"
                     /translation="MQSPLVVSRGAVDAYEKASGFIGIGRFFEEQGLLTIRKEEPCEE
                     SA"
     gene            complement(2274..3494)
                     /locus_tag="PKM87_08830"
     CDS             complement(2274..3494)
                     /locus_tag="PKM87_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNQ30208.1"
                     /translation="MSTAPLGTERSFHSIRRDSAERAITAALESHQITARDAALIREY
                     LGELKSCSGISDGRVNKNAYIMVGWRRYLGPYDSLTIGDIFAGRTAFENSRNGRGTPY
                     KQNTRHDWIKELKRFLIWLIENEYLTLPLAKVQKIRTPPTDPSTKVTGDLLTPEEIKA
                     MIRACSRSADRAIIMTLYEGGFRIGEVGRMRWGDLKFDEYGVVVNVTFKTGRPRYIRL
                     VMAREYLAQWRADYPHTAEGRAFVFLNTRGQELIHATISSRLERIAKRAGIQKHFTPH
                     IFRHSRITHLITQGMSESVIKLMMWGNLTTNMFETYAHLSGADIDAEVLRNYGIKNTG
                     PRKEEAKMEPIQCKVCMNINPPVSQYCSLCGLALTDEAAAEVEKVQRLVVSHPDDVKS
                     YLDRRGEVAGVVRG"
     gene            complement(3558..3630)
                     /locus_tag="PKM87_08835"
     tRNA            complement(3558..3630)
                     /locus_tag="PKM87_08835"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3595..3597),aa:Thr,seq:tgt)
     gene            3846..4568
                     /locus_tag="PKM87_08840"
     CDS             3846..4568
                     /locus_tag="PKM87_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30209.1"
                     /translation="MGDLALLVLEGPWWTPAHKPKRPSVLPFLEGLEKYKGNFNIYYS
                     SFYEKESFRKALEDDLAHTREQRLFLYIAAHGGSRMVGALEGEGGKTISSGMRLTTLL
                     RGVRRVARTTNIEGVLLGSCTLGANRTDLLATLKTSPIAWIFGYACEIDWIPSTLIDL
                     SILEHAMALKKEDLRSRTKIVGAFSSALSRFSGEFPICKEENRSVPLKYAISLLAKPR
                     KPHAVPEDLTRALLGSLGWDTP"
     assembly_gap    4918..5017
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5018..5267)
                     /locus_tag="PKM87_08845"
     CDS             complement(<5018..5267)
                     /locus_tag="PKM87_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF504 domain-containing protein"
                     /protein_id="HNQ30210.1"
                     /translation="MRKSREILLRCYHDPAFVFSNVQVCYIDRGAPGNESCVPGERIH
                     ALDSQYMEIGEGPESSPIPYHRITRIMYQGEVLWERQGK"
     gene            complement(5264..6997)
                     /locus_tag="PKM87_08850"
     CDS             complement(5264..6997)
                     /locus_tag="PKM87_08850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNQ30211.1"
                     /translation="MEQRIAGSIVEREVMAEMRDGTMLAADVYRPAWGEGPWPVLLIR
                     QPYDKAHAETFSYLHPSWYAAQGYLVVSQDIRGRWASGGEFSPFRHEAEDGYDTVEWA
                     AALPGSSGKVGMYGFSYGGLTQLMAATLAPPHLTCICPGFCTPQLFEGWAYNGGAFAL
                     SFNLTWALYLATDTARRKGLSGYEATLWKSIQSMHDWWQWLPMESFPLLRAHDFAPYF
                     FEWIDHPSYDAYWQRWSIERVYSRINVPALHIGGWYDIFRDGDLRLYEGLRSQAGSGK
                     ARENQKLVMGPWYHNPWNRVLGPCDLGDAAASRVDELQVQWFEYWLKGLGNGIMEEPR
                     VRYFMLGENRWRDDASWPPAKAEPVTLYLHSGGRANSLSGDGALTRELPGDEAPDVYV
                     YNPRDPVPSLGGQSCCIPGITPMGPADQRMVEAINQVLVYTMAPLETPFCIAGSVSVR
                     LFAATDVPDTDFCVKLVDVFPDGRAINLTSGILRAKFRDSLEHPHLLSPGIVDDFRID
                     GGSTAAQIRKGHRIRLEVTSSNFPCWDRNTNSGNNPMKDTYADIRVATTQVFHDRAYP
                     SCMVIHGWEER"
     gene            7422..8840
                     /locus_tag="PKM87_08855"
     CDS             7422..8840
                     /locus_tag="PKM87_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNQ30212.1"
                     /translation="MEGPGSLRQRLFRTKTIAELKEFCAGEHALKKAIGPIELVFFGI
                     GAVIGTGIFVITGIAAANYAGPALVISFVISGAACLFAALCYAEFATMVPVAGSAYTY
                     SFASLGEIWAWIIGWDLILEYSVSIAVVAVGWSGYMASFLAGAGLALPPAFTNPPGVD
                     GGILNVPAILIIALITLLLITGVKGSARVNTLIVAVKILVVLFFIAIGIGYIDTANWT
                     PFLPFGWMGVITGASIVFFAYIGFDAVSTAAEEVKNPERDLPIGIIGSLVICTILYIA
                     VSAVLTGIVPYPLLAQTSAPVAFALLQIGVPWGAELISAGAVLGITSVMLVLLYGQSR
                     IFFAMARDGLLPRSFATVHKTLRTPVRASLLVGAVTAVMASLLPLESMAELVNIGTLA
                     AFIIVAAGVLVLRRVHPDLSRPFRVPLVPLIPVLCILFCLGLILALPTITHLRFIVWL
                     AIGLVLYFFYGIRHSRYAEHAS"
     gene            8904..10133
                     /gene="mmp10"
                     /locus_tag="PKM87_08860"
     CDS             8904..10133
                     /gene="mmp10"
                     /locus_tag="PKM87_08860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107346.1"
                     /note="Mmp10 (methanogenesis marker protein 10) is a
                     cobalamin-requiring radical SAM methyltransferase that
                     creates the methylarginine modification to methyl coenzyme
                     M reductase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl coenzyme M reductase-arginine
                     methyltransferase Mmp10"
                     /protein_id="HNQ30213.1"
                     /translation="MTHLSVDIGGRPGIDCRGFCEYCYFRHVKETAPLGCRYCPPYKK
                     GCDYCSRSVREYYRGFRDLREIADDVLARLQVIPDEVSRVTISGGGDPSCYPCFTDLV
                     ELLSSMEAPLHIGYTSGKGFDDPGIGRFLVDSGLSEVSFTIFSVQPELRKQYMHDPTP
                     GASLAVLETLCGEIDVYAACVVLPGINDGPILEETCEWLEERGAKGIILMRFANRADQ
                     GLVLGNAPVIRGQKVHGVEEFRDMVTDLSARFRIKVSGTPLWDPEIGSPFALAREPDL
                     LKKLPKLERKASIITGRIAAPFIREILSSRGKGARVVPVEKDIACLITIGDLANLDPA
                     TLESLVIIPGRAFVYDREAEEVLSRDNIARTVMRGPDTLTADAETSMGMTRNEVLALE
                     MDAFRDLILQVNRYGST"
     gene            10332..10790
                     /locus_tag="PKM87_08865"
     CDS             10332..10790
                     /locus_tag="PKM87_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNQ30214.1"
                     /translation="MARMHARRRGHSGSVRPFRKEAPSWANTDVAAIEKIILDLRKEG
                     VSTSRIGMILRDRYGVPDVKLVTGKRIGQILKEKGMQPEIPEDLRNLIVKALGLRKHL
                     GENKSDLHNKRQLQLTESKVRRLVKYYTGNGRLPKDWSYKPESAEILLSR"
BASE COUNT         2062 a         3340 c         3221 g         2132 t
ORIGIN      
        1 tgatcgccct tccgggagac cgtgccgtag agtttccaga tgcgggccgc gttgtggttt
       61 gcacaatcaa ccgagcagtc cgagtccgag aagattgtgt ccagcacctc caggcaccgc
      121 ttcaccaggt cgcggctcgc gtcatcgttc gggagatcga tccggtacag gagatgggcg
      181 ccattccccg agtcggcaag gaccggggcc ggccaccctc gctccctccc gagatacgcg
      241 gcgacctccg cggccttgac aatcgccgcc tgatgctctg cgtcagtgga ggagacccct
      301 gaggggcgga ctgggtcgat atcaaccggg aaccatcgcc tgcggagaat atcgccgtca
      361 gcagtcgtcg cgtccttctt cccgagccgc accttgatcc ggttcgcccg ccgggagagc
      421 agcgcctggt tcacctcgtt cagggtaacg tagatgccct gcgttccgct cgcatccagt
      481 ggttcaacgg cagcagctaa ggcgtccagg tcatcgaagt aaccggaggc cattccttcg
      541 tccgtgatcg cacggacctc caccacctgc cccggtgcac agagtacgga aagcgcctcc
      601 cgaatcccgg cagccatccc ctcactccgc ggagacccgc tcgacacgcc aggcgtcctg
      661 gctttcgccc gggacaccct gccgcttcac cagccggaac ccctccgcct cgaagatctc
      721 gtgccggaga cagtggtcca ggatctcgct cctctccttc tcgcacgccg cgaggtcccg
      781 gtgcagccgg tcgatgctcc actccaggcc acgcatccgc tcaaggagca gccgctcgtc
      841 agagaccggg atgagctcaa gcgtcatctc cgctcacctc cgcgatgccg ccgacatgga
      901 tggtgaagat gcgccagccg ccggcatccg tgtagagcca gagctggacc gggagctcga
      961 tgcgccggag cattgcctgc aggcgggtga tctcgccggg gtagcgggag accaccgccc
     1021 gggcatccgc gaggggccgg cgggtccgcc tcgcctggac gaagaggacc tcgcggtgcc
     1081 gcccggcgac cagatccacc ggggcccggg acccggcact ccggatcacg cagaaccccc
     1141 ggctccgcaa ccggtcccgg gcaagatact ccacgcggcg gccgcgggtc cgctcaaggc
     1201 tctgcatgga caagcctccg ggggaccacc cagatctccc cgacaggagc aaacgcccgg
     1261 gcaaaccggg tatgctcccg gcccacgtag aagagggcag gggagaaggt cgagccggtc
     1321 cgcggccctt ccccctcgaa gtcgatccgc cgggtgggga aacagatcac atttgcggac
     1381 ccggtcagga tgcgccaggc ccgggtctcg gtcgccgact tccagagtac gacggccgca
     1441 accgtccggc cggcacagta ctcagcggcc agcttctgga accagcggtc cacgtcccgc
     1501 ccgaacggcg ggttcaggaa gacccggccg tcccagggct gggagagccc gtcatccccg
     1561 cgagtgaagt ggcgtgcggc cggcacgttc gggcagcacc ggctgttgct ggacgggtcg
     1621 aggtcgatgc cccccaggca ctcgaggacc gcgtccagga tataccgggg tgtgagatgc
     1681 tcatcggtcc tgtgggagag gagggcagag gggaccgtca tgccactccc cccggtgtcc
     1741 gggttccgca caggagattc cacgggtatg cgtgaaatcc cgggatgccg cggctcatgc
     1801 cgggaggaag agggggcgcc accccatgcc ggcaaatact caaatagttc aacagatcat
     1861 actcagctct ggttcccttc gttacggtgc ggtggaacga ctgttgatga ggggaaccac
     1921 tcatgcgctt tcctcacatg gttcttcttt ccggatagtc aggagtcctt gctcctcgaa
     1981 gaaacggccg attccaatga atcccgacgc cttctcgtag gcgtcaaccg ctcctctgga
     2041 gacgacaagt gggctttgca taatgacacc ttctttgtca gtagtgtgtg accgcacatc
     2101 aggcgaaggt ctccgccctg gagggctcgc gtgcgagaat aggcctatat gttactcacc
     2161 gagcccggtc tggtgtgtgg atcacacgga aaaagatggg agaatggggt atatgaacct
     2221 gaaggagggc atttgaaagt ggaaaaaggg taataaggct taccgtatcg tttttagcct
     2281 cttaccaccc cagccacttc tccccggcgg tcaagataac tcttcacgtc gtcggggtgg
     2341 ctgaccacga gcctttgaac cttttcaacc tccgcagccg cctcatcagt cagggccagc
     2401 ccgcagaggg agcagtactg ggagaccggc gggttgatgt tcatgcagac cttgcactgg
     2461 atcggctcca tcttcgcctc ttctttccgg ggcccggtat tcttgatgcc gtagttgcgg
     2521 aggacctcgg cgtcgatgtc cgccccgctc aggtgggcat aggtctcgaa catgttcgtc
     2581 gtgaggtttc cccacatcat cagcttgata acactctcgg acatcccctg cgtgatcagg
     2641 tgcgtgatgc gggagtggcg gaagatatgc ggggtgaaat gcttctggat gcctgcccgc
     2701 ttcgcaatcc tctccagccg gctgctgatg gtcgcgtgga tcagctcctg gccccgcgtg
     2761 ttgaggaaga caaacgccct cccctcagca gtgtggggat agtcagcccg ccactgggcg
     2821 agatactccc gggccatgac caggcggata tagcgcggcc tcccggtctt gaaggttacg
     2881 ttgacgacca cgccatactc gtcgaacttg aggtctcccc accgcatccg gccgacttcg
     2941 ccgatgcgga aacctccttc gtagagcgtc atgataatcg cccggtcggc actgcggctg
     3001 caggcccgga tcatggcctt gatctcctcg ggagtgagga ggtccccggt caccttggtg
     3061 ctcgggtccg tgggaggtgt ccggatcttc tggacctttg ccagcgggag ggtgagatac
     3121 tcgttctcga tcagccagat gaggaaccgc ttgagttcct tgatccagtc gtggcgggtg
     3181 ttctgcttgt agggtgtccc cctcccgttc cgggagttct cgaaggccgt gcggcccgcg
     3241 aagatgtctc caatggttaa cgagtcgtag gggccgaggt agcgcctcca gccgaccatg
     3301 atgtaggcgt tcttgttgac ccggccgtcg ctgatgccgg aacacgattt gagctcgccg
     3361 aggtattccc ggatgagggc ggcatccctg gcggtaatct ggtgcgattc cagtgcagca
     3421 gtgattgctc gctctgcact gtcccttcgg atcgagtgga agctccgctc ggttcctaac
     3481 ggtgcggtgg acatgaaatc tacatttcgg ctcaaggtaa aaaaaggttt tgcaagggcc
     3541 tttgaacgaa tgcggcaagc cccagccgtg atttgaacac gggacctgct gattacaagt
     3601 cagccgctct gccagctaag ccactggggc gccatatact gttgccatcg gagagtgaaa
     3661 aagatggcga cggaagaagt gttacacagt gaggcaggat tctcctactt actcttccat
     3721 gacctcaaca tctgagcgat aggagagatc gaatatcact ctgcaatctt accttcgtct
     3781 attccccttt ttacaatccg gcaaaccaaa ccttgatatc acatccggat gagtgctgat
     3841 tcacaatggg cgatttagca ctccttgttc tggaaggtcc gtggtggacg cctgcccaca
     3901 agcccaagcg cccctccgtg ctcccgtttc tggaagggct cgaaaagtac aagggcaact
     3961 tcaacatcta ctactcgagc ttttacgaga aagagagctt ccggaaggcg ctcgaagacg
     4021 acctcgccca caccagggag cagaggctct tcctctacat cgcagcccac ggcgggagca
     4081 ggatggtcgg ggcgctggag ggcgaaggcg gaaagaccat cagcagcggg atgcgtctca
     4141 cgacgctgct tcggggtgtc aggagggtgg cacggaccac gaatatcgag ggggtcctgc
     4201 tcggctcctg caccctcggg gcgaaccgca ctgatctcct tgccaccctg aagacctccc
     4261 ccatcgcctg gatcttcggg tatgcctgcg agatcgactg gatcccgagc acgctcatcg
     4321 acctctccat cctcgagcat gccatggcgc tcaagaaaga ggacctccgg agcaggacaa
     4381 agattgtcgg ggcgttctca tccgcgctat ccaggttcag cggcgagttt cccatctgta
     4441 aggaggagaa ccggtccgtc cccctgaagt atgccatctc acttcttgcc aagccgcgga
     4501 agccccacgc ggtgcccgaa gacctgacca gggcgctcct tgggtccctt gggtgggaca
     4561 ccccctgatc ccggaaggac ggcccatgcc ccggcgagaa cggtcatcga tatgactatt
     4621 gccgcccttc ctctagggag ttcgtcaggt acatcgggct ctccgcgaat gtaagtaaag
     4681 atttaggaac cccgggccca ttatgcaggg agcagtcatc ccgcacccct cttcgtgata
     4741 cacgctgcgt ggggaagccc cggagcgatc ctggataggt atgcaaagat cgctcaacct
     4801 caagtcaccc acatggcaaa gcgacgaggc gtataagaga gagggcgcct gctactcact
     4861 gtgtttcccc tgccgttccc agagcacttc gccctggtac atgatgcggg tgatgcgnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtt tcccctgccg ttcccagagc
     5041 acttcgccct ggtacatgat gcgggtgatg cggtgatacg ggatgggaga cgactccggt
     5101 ccttccccga tctccatgta ctgcgaatcg agggcatgga ttctctctcc gggaacgcat
     5161 gactcgtttc ccggcgcccc cctgtcgatg taacagacct ggacgttgga aaaaacgaat
     5221 gccgggtcat ggtagcaccg gagtaggatc tcgcggctct tcctcaccgc tcctcccacc
     5281 cgtggatcac catgcacgaa gggtaagccc ggtcgtggaa gacctgggtt gtcgccaccc
     5341 ggatgtccgc ataagtgtcc ttcattgggt tgtttccgga gttggtgttt cggtcccagc
     5401 aggggaagtt gctgctcgtc acctcgagcc tgatgcggtg ccccttccgg atctgcgccg
     5461 ccgtgctccc gccgtcgatg cggaaatcat cgacaattcc cggagagaga aggtgcggat
     5521 gctccagcga gtcccggaac ttcgcacgga ggatgccgga tgtgaggttg atcgccctcc
     5581 catccgggaa cacatccacc agcttcacgc agaagtcggt gtctgggaca tctgtggcag
     5641 caaagagccg cacggatacg ctccccgcaa tgcagaaggg cgtttccagg ggggccatgg
     5701 tgtacacgag cacctggttg atcgcctcca ccatccgctg gtcggcaggc cccatggggg
     5761 tgatgccggg aatgcagcag gactgccctc cgagacttgg gaccgggtcc ctggggttgt
     5821 aaacgtagac atcgggggcc tcgtcccccg gcaactcgcg ggtgagggcc ccgtcgcccg
     5881 aaagggagtt cgccctccca ccgctgtgga ggtaaagggt cacgggttct gcctttgccg
     5941 ggggccagga ggcgtcatct ctccatctgt tctcgccaag catgaaatag cggacccgcg
     6001 gctcttccat gatgccgttc ccgagcccct tgagccagta ctcgaaccac tgtacctgca
     6061 gctcgtccac gcggctggcc gcggcatcgc caaggtcgca ggggccgaga acccggttcc
     6121 agggattgtg gtaccagggg cccatcacca gtttctggtt ctcccttgcc ttccctgacc
     6181 ctgcctgtga ccggagcccc tcgtaaagcc ggaggtcccc gtccctgaat atgtcgtacc
     6241 acccgccgat atgcagggca gggacattga tccgggaata gacccgttcg atgctccacc
     6301 gctgccagta ggcgtcgtat gagggatggt cgatccactc gaagaagtat ggcgcgaagt
     6361 cgtgggcacg gagcagggga aacgactcca tcgggagcca ctgccaccag tcgtgcatcg
     6421 actggatcga tttccagagg gtggcctcgt acccggaaag tcccttacgc cgggcggtat
     6481 cggtcgcgag gtatagtgcc caggtcaggt tgaaggagag cgcaaaggcc ccgccgttgt
     6541 aagcccaccc ctcgaagagc tgcggagtgc agaaccccgg gcatatgcag gtgaggtggg
     6601 ggggagcaag agtggcagcc atcagctggg tcaggccgcc atacgagaac ccatacatcc
     6661 ccacctttcc agacgaccca gggagcgcag ccgcccactc cacggtgtcg tacccgtcct
     6721 ctgcctcgtg gcggaacggg gaaaactcgc cgccggaagc ccaccggccg cggatgtcct
     6781 gggagaccac gaggtagccc tgggcggcgt accacgatgg atgcaggtag gagaacgtct
     6841 cggcatgggc cttgtcatac ggctgcctga taagaaggac cggccatggg ccctctcccc
     6901 aagccggccg gtatacgtcg gccgcgagca tcgtcccgtc tctcatctcg gccatgacct
     6961 cccgttccac gatggatccc gcgattcttt gctccatgat tcggcgttcc tccatggaaa
     7021 acagggactg gcactattta tgcccttctc cggataatcg gtcattccgg tttacgatat
     7081 aaggctggac gagacgaaca gaaaatctcg tcccggttcg gactgatgga aaatctttct
     7141 tgcatctgga attcagccgg gaaagaggcc tgcgaccccc gatgagtgcc ggtcgcctgc
     7201 aggtccctga tcctcatgtc ccataaattc cctgtggggg caaggtgctc atcctggttc
     7261 ctgtcagaac tccaatcttt cttgcaatgg cgggcacctg gttcgtatcc ggataccggg
     7321 aaagatccag tgcctcctta cccttatgcc cctttttgaa taatgcaggc aactaaaaaa
     7381 aatacttggt tttaagaggc cgcaatgcca tccctcttac catggaaggc cccggctccc
     7441 tgcggcagcg gctttttcgc acaaagacta tcgctgaatt gaaagaattc tgtgccgggg
     7501 agcatgccct caaaaaggcg attggtccca ttgagcttgt ctttttcggg ataggcgccg
     7561 tcatcgggac cggaattttt gtgatcaccg gaatcgctgc ggcgaactat gcagggccgg
     7621 cgctggtcat ctcattcgtc atttcgggtg ctgcgtgcct tttcgccgca ctctgttatg
     7681 ccgagttcgc gacaatggtc ccagtggcgg gcagcgccta cacctacagc ttcgcctcac
     7741 tcggtgaaat ctgggcctgg ataatcgggt gggacctgat actcgaatac tccgtctcca
     7801 tcgccgtcgt cgccgtcggc tggtcgggct atatggcaag ctttcttgcc ggtgcgggcc
     7861 ttgcgttgcc tccggcgttc acgaaccccc cgggagtgga tggaggcatt ctgaatgttc
     7921 ccgcgatact catcatcgcc ctcatcaccc tcctcctgat caccggcgtg aaagggagtg
     7981 caagggtgaa cactctcatc gtcgcagtca agatactcgt agtcctcttc ttcatcgcca
     8041 tcggaatcgg atatatcgat accgccaact ggaccccctt cctgcctttc ggatggatgg
     8101 gggtaatcac gggggcatcg atcgtattct tcgcctatat cgggtttgat gccgtttcta
     8161 ccgctgcaga ggaggttaaa aatccggaac gcgaccttcc aatcggcata atcggatcgc
     8221 tcgtcatctg cacgatcctt tatatcgccg tatctgctgt tctgacaggg atagtgccct
     8281 atccgctgct cgcccagaca agtgcgccgg ttgcgttcgc cctcctgcag attggggtcc
     8341 catggggagc ggagttaatt tctgccgggg ctgtccttgg aatcacgtcg gtgatgctgg
     8401 tgctcctgta tggccagtca aggatcttct ttgcgatggc ccgcgacggc ctgctccccc
     8461 ggagttttgc caccgtccat aagacattac gcacacccgt gagggcgagc cttctcgtcg
     8521 gtgcggtgac tgcagtgatg gcttcgctgc tcccgctcga gagcatggcc gaactggtga
     8581 acatcgggac gctggcagca tttatcatcg tcgctgccgg agttctggta ctgcgacggg
     8641 tccatcctga cctttcacgg cctttccgcg tacctcttgt tcccctgatc cctgtcctgt
     8701 gcatcctctt ctgccttgga ttgatcctgg cactcccgac tattacgcac ctgcgtttca
     8761 tagtctggct tgcaatcggg ctggttctgt acttctttta tggaatccgg cattccagat
     8821 atgcagaaca cgcatcctga tagtggtgtg tcatgtatgg atagcagcca ttatgcctcg
     8881 ctgtcaccca cattctacta cgaatgacac acctctcggt ggacatcggg ggacggccgg
     8941 ggatcgactg ccggggcttt tgcgagtact gctatttcag gcacgtgaag gagactgccc
     9001 cgctcggctg ccgctactgc ccgccctata aaaagggatg cgattactgt agccggagcg
     9061 tgagggagta ctaccggggg ttccgggacc tcagggagat cgccgatgac gtccttgccc
     9121 gcctccaggt catccctgac gaggtgagca gggtcaccat cagcggcggc ggagacccga
     9181 gctgctaccc atgcttcact gaccttgtcg agctcctctc cagtatggag gcgccgctgc
     9241 atatcgggta tacgagcggg aagggattcg atgatcccgg gatcggaagg ttcctcgtcg
     9301 attcaggcct ctccgaggtc tcgttcacca tcttttccgt gcagccggaa ctgcggaagc
     9361 agtacatgca cgaccccacg cccggggctt cgctcgcggt gctcgagacc ctctgcgggg
     9421 agatcgacgt ctacgccgcc tgtgtggtcc tgccgggcat caacgacggc cctatcctcg
     9481 aggagacctg cgaatggctc gaggagaggg gtgcaaaggg gatcatcctg atgcggtttg
     9541 cgaaccgggc ggaccagggc ctggtgctcg gaaacgcccc tgtcatccgc gggcagaagg
     9601 tgcatggggt ggaggagttc cgggacatgg tcactgacct ctcggcccgg ttccgcatca
     9661 aggtgagcgg cacccccctc tgggaccctg agatcggctc cccctttgca ctcgcccgcg
     9721 agcccgacct cctgaagaaa ctccctaaac tcgaacgcaa agcgagcata atcacaggga
     9781 ggatcgccgc accgttcatc agggagatcc tctcctcgcg ggggaagggc gcaagggtgg
     9841 tgccggtcga aaaagacatt gcctgcctga tcaccatcgg cgacctcgca aacctcgatc
     9901 ccgccacgct cgaatccctg gtgatcatcc ccgggagggc gtttgtctac gaccgcgagg
     9961 cagaagaagt gctctccagg gacaacatcg cccggaccgt gatgcggggg cccgacaccc
    10021 tcaccgcgga cgccgagacg agcatgggga tgacccggaa cgaggtgctg gcgctcgaga
    10081 tggacgcgtt tcgggacctc atcctccagg tcaaccgcta cggaagcaca tgaccgccct
    10141 gtccgtccac gatagcatta atattccccc gcccaaatgg attgaagcac tcctggatag
    10201 tgctggcaga tgtaagtccg acgtgcggca acgcacgggc cggagggggt tctcccccgg
    10261 cctctgggat tacggagagt cagcgtgact cttttcggat ttacattgcg taatcggagg
    10321 tgaataccac aatggcaaga atgcatgcac gacgcagagg gcactcggga tccgttcgac
    10381 ccttccgcaa ggaggcccct tcctgggcca acaccgatgt cgcggcgatc gagaagatca
    10441 tcctggacct gcgcaaggag ggcgtctcca ccagcaggat cgggatgatc cttcgtgacc
    10501 gctacggtgt cccggacgtc aagctcgtca ctgggaaacg gatcgggcag atcctcaaag
    10561 agaaagggat gcagcccgag atccccgagg accttcggaa cctgattgta aaagcgctcg
    10621 ggctccggaa gcacctgggc gagaacaaga gcgacctgca caacaagcgg cagctgcagc
    10681 tcaccgagtc caaggtccgc cgactggtaa agtactacac cgggaacggc cggctcccga
    10741 aggactggag ctacaagccg gagagcgccg agatcctcct ctcgagataa tcccccatgt
    10801 cccttgacga ggccgcacag aggctcgccg aacacctcgg caggcaggag ttcgt
//
LOCUS       DAOQYR010000076        10761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_6592_length_10761_cov_8.542219, whole genome shotgun sequence.
ACCESSION   DAOQYR010000076 DAOQYR010000000
VERSION     DAOQYR010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 10761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10761
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6592_length_10761_cov_8.542219"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PKM87_08870"
     CDS             complement(<1..199)
                     /locus_tag="PKM87_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HNQ30215.1"
                     /translation="MPEKVYFTGVRAREPGQNTISKIQRLFDAAGFPRMIEPGDLTAI
                     KLHFGEWGNESYISPVHVRQVV"
     gene            complement(354..842)
                     /locus_tag="PKM87_08875"
     CDS             complement(354..842)
                     /locus_tag="PKM87_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNQ30216.1"
                     /translation="MLKKSMEEALNKQVNAELYSSYLYLSMAAYCETQLMKGFAKWLR
                     LQADEEREHAMKFFDYIIEAGGTVKLAKIEAPKTGWKSIGEVFDEVYEHEMKVTGLIG
                     GLMDLAIKEKDYATRNFLGWFVKEQVEEEAHASEIQGKIKMLGDVVGHLFWLDHELGK
                     RE"
     gene            complement(1049..1789)
                     /locus_tag="PKM87_08880"
     CDS             complement(1049..1789)
                     /locus_tag="PKM87_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30217.1"
                     /translation="MSKYSWQIKLGIVLLASSFFIYSLKFLFLGDPENTYYYVFNALG
                     FLPINVLLVTLVLNELLTVRARKERLEKLNMVIGTFFSEAGTDLLKTIARADHTISTL
                     EDTLRVTGDWKDEDFLRVRKVMEGYDFHVDITRIDLGNMREYLKERRNFLLRLLENPM
                     LLEHQTFTEVLRAVFHFTEEIERRDALACMTERDCMHLKGDVERIYRLLSLEWLAYMK
                     HLHAHYPYLSSLAMRTNPFDSKASVVIG"
     gene            complement(1852..2979)
                     /locus_tag="PKM87_08885"
     CDS             complement(1852..2979)
                     /locus_tag="PKM87_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30218.1"
                     /translation="MDNSGGAVPSAGVAGGDFMQWQEEDLAARLPAVVKGRQDAGLDG
                     LVGGLEAVVINTEPERLEPGVDELLHFTGLGIAERFVDSNATAYVLATRDSPSIVIMS
                     RGRGANPFHPFNQAEKARALPNTRLETFIFRARDLDTYVGIQRELGVRFLTERIVDAP
                     EYRFIQTYPSRFTGNSLGFLEWKGSGRSFAGAGAKRLPITQRKPDLPWLSQIHGLDHT
                     ATRVRAVERNSAIIEFLSLTGYHFDFAVFVKSLNSITSVARLSRDDFAMVFTSGIAPY
                     TDDEASGPTEKFIRSYGTRVHHMAFRTDEIEEVFGRLRGSGMDFLVDLVGSPDEGLKQ
                     TFSMPSPYTMVVNEYIHRYEGFDGFFTRSNVEALTRATGRQ"
     gene            complement(3112..3255)
                     /locus_tag="PKM87_08890"
     CDS             complement(3112..3255)
                     /locus_tag="PKM87_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HNQ30219.1"
                     /translation="MAIDPICKMTVDEKTAKFTSQYKGKTYYFCAPGCKKKFDADPEK
                     YLI"
     gene            complement(3260..5659)
                     /locus_tag="PKM87_08895"
     CDS             complement(3260..5659)
                     /locus_tag="PKM87_08895"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNQ30220.1"
                     /translation="MHCATCAANIEKSLATVGKAERAEVNFGTDTARVEYDPAHATLS
                     DIERAVREAGYEVVFEQATLKVGGMVCATCVETIETALSALPGVVRAQVNLATEKAQV
                     TYNSSLTTLQDMRAAIEDAGYQYLGLADQLTVEAEEEARRRDLDDKFRRFMVGFAVSI
                     PLFALMYIPLPISMHALSYLMLAVSTPVFIYVAYPIFRAARMAARNRTLNMDVMYAMG
                     TGVAYVSSVLGTLGIVLTHEFMFYDTAIMLASFLILGRYLEARAKGRTSAAIRALMGL
                     RPKTALLVTGEGEKEVPIEDVQVGDRIRVKPGGKVPVDGTVTEGESYVDESMITGEPI
                     PVLKAAGAQVVAGTLNTNSIFTFRAEKVGKDTVLAQIIRLVEDAQASRPPVQRIADVA
                     VSYFIPTVLAIAFAAFLVWYLVIGSTLLFALTALVSVLVVACPCALGLATPTAVTVGV
                     GRGAELGVLVRNGEALETADQVNTVVFDKTGTLTRGKPAVTDIFPSGIDQETLLGIAA
                     AVEKNSQHPLADAVVQRTLLEGAPIGEAEGFDTHGGKGVSARVLGEEVLVGNRAFLEE
                     KGVVLAQDRESVVRGLQNEGKTVILIAVGGNLAGVLGVADTVKETTPSALEVLKGMGM
                     KVAMITGDNRLTATAIGKRLGIEEVFAEVLPGDKAKEVEKLQKAGNIVAFVGDGINDA
                     PALARADVGIAIGSGTDVAIESGDIVLVRDDLLDAAAAIQLSRKVMSRIRQNIFWAFA
                     YNAALIPLAAGIFYPFTGYTLRPELAALAMALSSVTVVTLSLSLKRYIPPAKRYHSLK
                     V"
     gene            complement(5804..7045)
                     /locus_tag="PKM87_08900"
     CDS             complement(5804..7045)
                     /locus_tag="PKM87_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNQ30221.1"
                     /translation="MEPAFPVPPVPIIVLAAVFLLIAVRQVGRFRLRIWQVMLGGALA
                     VLFLGQIAPIEALRSINLDVMIFLFAMFVVGEALERSGYLHSLSFRLFARARGSGQFL
                     LLVLLGMGFLSALLMNDTVAIIGTPIVIAYACRFGVHPKTALLALCFAVTIGSVMSPI
                     GNPQNLLVATYSQMPQPFLAFLLYLGVPTIVNLVIAYLLLTRLAPMGAIECAYDETEG
                     QVSDERLAALSRVSLAVILVLIGIRVLTGPAFIPLALIAVAGALPVVIFSSQRAAIVR
                     GIDWPTLVFFASMFVLMQSVYQSGFFQSGLDFTGLQSVPLILAASVTFSQFISNVPFV
                     ALFQPLIAQQGLSIEGVMALAAGSTIAGNLTILGAASNVIVVQNAEKRGYTLTFWEFF
                     RVGLPLTIANCVVYWVFLSLV"
     gene            complement(7053..7745)
                     /locus_tag="PKM87_08905"
     CDS             complement(7053..7745)
                     /locus_tag="PKM87_08905"
                     /EC_number="1.1.1.302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone
                     5'-phosphate reductase"
                     /protein_id="HNQ30222.1"
                     /translation="MLRPYVVVNLAMSADGKLSTRERRQVKISGKEDFSRVDVLKAES
                     DAIMVGIGTVLADDPSLTVKSAELIAARRARGLSDHPVRVVVDCRARTPLDSSILRKG
                     AGKRVVACCQDTDDERCRALSSLACVIRAGEGKVDLEALLSELYRMGIRRLMVEGGGR
                     LIGALFSLGLVDEYMTFIGNMIIGGEMAPTPADGPGADREAQFTGLSLYSVERMDQGV
                     LLRWMVEKRSPE"
     gene            7923..9230
                     /gene="ablA"
                     /locus_tag="PKM87_08910"
     CDS             7923..9230
                     /gene="ablA"
                     /locus_tag="PKM87_08910"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HNQ30223.1"
                     /translation="MTISENHIHSFSSCQDVASTSSWTDWRWHIRHSVRDIATLESLL
                     GIEFEKVERDAMERTIEKFPMAITPYFLSLIEPDHYRNDPIFRQSVPSPDELLIEEHD
                     IEDPLHEDKDSPVPGITHRYPDRVLFLVSNTCAMYCRHCTRKRRVGDIDSVPSEQEIR
                     KGIEYIRDTPAIRDVLLSGGDPLMLPDDYLKWILDEIHDIPHVEVIRIGSRIPAVLPF
                     RVTDSLLSILRKHHPVWMNVQFNHPREVTPSSCRALGKIADAGIPLGNQSVLLAGVND
                     CPRIIRALVHHLVKNRVRPYYLYQCDLSEGLSHFRTPVAKGIEIMENLIGHTSGFAVP
                     TYVIDAPGGGGKIPMMPNYTVSSGVNRVVLRNYEGVICTYPEPDTYQRRTCDGDCVHC
                     MLDHENPEGIEARPVGIAKLLADYDNASTLVPADTPRMERRNE"
     gene            9223..10053
                     /gene="ablB"
                     /locus_tag="PKM87_08915"
     CDS             9223..10053
                     /gene="ablB"
                     /locus_tag="PKM87_08915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative beta-lysine N-acetyltransferase"
                     /protein_id="HNQ30224.1"
                     /translation="MSDTICRYGSSTYQHGPLNNRVYLMRVTSKEIPEIFDIIEELAL
                     TSKYAKIVAKVPVSLSGDFLQRKYQKEALIPRFFHGKEDCVFMSRFLSYRNGKIPIPD
                     NSMYAIRGRGPVVNRTPVLPEGYRSQPCKESDVHALSALFSGVYETYPFPIYNPDFLL
                     AMMREGTRYHGIWFGDTIVAASSAEVDANEMNAEMTDFATLPAHRGLGLGRYLLSSME
                     QDLEARDIITLYTIARALSRQVNRLFFGAGYSYGGFLFRNTQICGSCESMNVWYRHLI
                     "
     gene            10205..>10761
                     /locus_tag="PKM87_08920"
     CDS             10205..>10761
                     /locus_tag="PKM87_08920"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNQ30225.1"
                     /translation="MYENTNSHLLDPALLIIDMQNDFVRPGTPLQVTGVSSIIPKVRD
                     LLSLFREKDLPVFHVLRVHRASGVDVEITRREIFAKTPFAVEGTAGAAVISELTPLNG
                     EHIVKKTRMSAFFQTDLDIMLRTLGVDTLFIAGIQTPNCVRTTVFDAMAYNYRVFLVR
                     DAVGAKTDAIHEANVLDMQNIGIGIV"
BASE COUNT         2369 a         3139 c         2896 g         2357 t
ORIGIN      
        1 ccacgacctg gcggacatgc acggggctga tataggactc gtttccccat tccccgaagt
       61 gcaacttgat cgcagtgagg tctccgggtt ctatcatccg gggaaacccg gccgcatcaa
      121 aaagtcgctg tatctttgat atggtgttct gaccgggttc acgtgccctc acgccagtaa
      181 aatatacttt ctcaggcatc gcagagttcc cctgctctca tcacaattgg gagtgagcat
      241 cttaataggg ctatccactc aggatgaatc tgaaggcaat tccaactatg gaaaattcac
      301 acgcattgtg atgcgggaac accaaaaaaa ccttgcaaga aaaagattga gggttattcc
      361 ctcttcccga gttcatggtc cagccagaag agatgcccga cgacatcgcc gagcatcttg
      421 atcttcccct ggatttcgct tgcgtgtgcc tcttcttcca cctgctcttt cacgaaccag
      481 ccaaggaaat tccgggtggc ataatccttc tccttgatcg caaggtccat cagcccgcca
      541 atgaggcctg taaccttcat ctcatgctcg tagacttcgt caaaaacctc gccgatggac
      601 ttccaccctg tctttggtgc ttcgattttt gccagtttga cggtcccgcc tgcctcgatg
      661 atgtaatcaa agaatttcat ggcatgttcc cgctcttcat ctgcctggag ccggagccac
      721 ttggcaaacc ctttcatcag ctgtgtctcg cagtatgcgg ccatggacag gtagagatac
      781 gatgagtaga gctctgcgtt cacctgcttg ttcagcgctt cttccatact cttttttagc
      841 aacagtattc ctccagaaca tggaggtatt gaccaagacg ggctttaatg gtttggttta
      901 tgcctcctgc ctgggaatgg cacgcggctc ccatggcagg agcattaggc tgtgtcatgt
      961 gcgactctcc cgtcccataa ccggataatt tatctcttct ggttattcgc cgctttatcc
     1021 cggagatctc aagaaaagat aggtggaatc aaccaatgac taccgaagcc ttgctgtcga
     1081 acgggttggt ccgcatcgcc agcgatgaga ggtaagggta gtgggcatgg aggtgcttca
     1141 tataggcgag ccattcgaga gaaaggagac ggtatattct ctccacgtcc cccttgaggt
     1201 gcatgcagtc cctctccgtc atgcaggcga gagcatccct tctctcgatc tcctcggtga
     1261 aatgaaagac agcccgcaac acttctgtga atgtctggtg ttcaaggagc atggggtttt
     1321 caagaagacg gaggaggaag ttcctcctct ctttgagata ttccctcatg ttcccgagat
     1381 ctatccgcgt gatatccaca tggaaatcat atccctccat gacctttcgc accctgagaa
     1441 agtcctcgtc cttccaatca ccggtcaccc gaagggtgtc ctcgagggtc gagatcgtat
     1501 gatccgccct cgctatcgtc ttgagcagat ctgttcccgc ctcggaaaag aacgttccta
     1561 tcaccatgtt cagcttctcc aggcgctcct tcctggcccg gacagtcagg agctcgttca
     1621 acacgagggt gaccaggagc acgttgatgg gaaggaaccc cagcgcgttg aagacataat
     1681 aataggtgtt ctccggatct ccgaggaaga gaaacttgag cgagtaaatg aaaaaagagc
     1741 ttgccaggag gacgatccca agtttgatct gccaggaata tttgctcatg ctaccactca
     1801 tctctcagcg cttcgcattc tctggagaaa tatgtgccgc atgcattggg atcactgtcg
     1861 cccggttgcc ctcgtcagtg cttccacgtt gctcctggta aaaaacccgt caaatccctc
     1921 gtacctgtgt atgtactcgt tgacgaccat cgtatacggt gacggcatgg agaatgtctg
     1981 cttgagaccc tcgtcagggg acccgaccag gtcaacgagg aaatccatgc cgctcccgcg
     2041 gagcctgcca aacacttctt cgatctcatc agtgcggaag gccatgtggt gaacacgggt
     2101 tccataggaa cggataaact tctctgtagg ccctgatgct tcgtcgtcag tgtagggtgc
     2161 gatcccggaa gtgaagacca tcgcaaagtc gtccctgctc aggcgggcaa cgctcgtgat
     2221 ggaattgagg gacttcacga atacggcaaa atcgaaatga tagcccgtga gcgagaggaa
     2281 ctcgatgatg gcgctgttcc tctcgaccgc cctcaccctt gtcgcggtat gatcgagccc
     2341 gtgtatctgc gagagccagg ggaggtccgg ttttctctgg gtgatgggga gtctttttgc
     2401 ccccgctccg gcaaacgagc gaccagatcc cttccattcg agaaaaccaa gcgagttccc
     2461 ggtgaaccgg gatgggtacg tctggatgaa ccggtactct ggtgcatcga cgattctttc
     2521 agtcaggaac cggacaccca gttcccgctg gatcccgaca taggtgtcaa ggtcccgcgc
     2581 ccggaagata aaggtctcga ggcgggtatt gggaagtgcc ctggcttttt cagcctggtt
     2641 gaagggatgg aaaggattcg cgcctcttcc cctgctcatt atgacaatcg acggtgaatc
     2701 cctggtcgcg agtacatagg cagttgcgtt ggagtcaacg aaacgctcag cgattccaag
     2761 gcctgtgaag tgcagcagtt catccacgcc gggttcaagc ctctcgggct cggtgttgat
     2821 gaccaccgcc tcaaggcctc cgaccagccc atcgaggcct gcatcctgcc tgcccttcac
     2881 cactgcgggc agccttgccg caaggtcctc ctcctgccac tgcatgaagt cccctccggc
     2941 aactcccgcc gacgggactg cccctcctga attgtccatt cctgcaccca cttttattgg
     3001 cagaaggtgc ggaaggttga atgataaagg ttgggaccgc ggaagcagca ggtggaggga
     3061 agggcctccg ccagtccagg gtgctgatcc tgcagttgcg ctcccatccc gctatatcag
     3121 gtatttctct ggatctgcat cgaacttctt cttgcagccg ggagcgcaga agtagtaggt
     3181 ctttcctttg tactggctcg tgaattttgc cgtcttttcg tcgactgtca tcttgcatat
     3241 ggggtcaatt gccatgcttt cacaccttaa gggagtggta tcgcttcgca ggcggtatat
     3301 accttttgag ggagagcgag agggtgacaa cggtcactga cgagagcgcc attgcaaggg
     3361 ccgcaagctc ggggcggagc gtatagccgg tgaacgggta gaagatccct gccgcgaggg
     3421 gaatgagggc cgcgttgtat gcaaatgccc agaatatgtt ctgtcgtatc cgcgacatga
     3481 ccttcctcga gagctggatg gctgccgcgg catcgagaag gtcgtccctg acaagcacga
     3541 tgtccccgct ctcaatcgca acgtcggtcc cgctcccgat cgcgatgccg acatcggccc
     3601 gtgcaagggc gggggcatcg ttgattccgt cacccacgaa cgccacgata ttgcctgcct
     3661 tctggagctt ttcgacctcc tttgccttgt ctccggggag aacttccgca aatacctctt
     3721 caatccccag cctttttccg atggctgttg cagtgagcct gttgtcaccg gtgatcatcg
     3781 ccaccttcat ccccatcccc ttcaggactt ccagtgccga cggcgtggtc tccttgacag
     3841 tatcggcaac gcccaaaacc cctgcgaggt tgcctccgac ggcgatcagg atgacggtct
     3901 tcccctcatt ctgcagaccg cggaccaccg attcgcgatc ctgtgcgagc acgaccccct
     3961 tctcttccag gaatgcccgg ttcccgacca gcacctcctc tccgagaacc cgggcagaca
     4021 cccccttgcc accgtgggta tcgaatcctt ctgcctcccc gatcggggca ccttccaaaa
     4081 gcgtcctctg taccaccgcg tccgccaggg ggtgctggga gttcttctcg acggctgccg
     4141 cgattccaag aagtgtctct tggtcgatcc ctgaagggaa tatgtcggtc accgcgggct
     4201 ttcccctggt gagggttccg gtcttgtcaa agacgaccgt gttcacctgg tccgcagtct
     4261 caagcgcctc cccgttcctg acaaggaccc caagttctgc acccctcccg accccgactg
     4321 tcaccgcagt cggtgtcgca aggccgagcg cacacggaca ggcgaccacc aggacggaga
     4381 cgagagccgt cagggcgaag aggagtgtcg agccgatcac gagataccat acgaggaacg
     4441 cggcgaaggc gatcgcgagc actgtgggga tgaaatagct gaccgccacg tctgcaatgc
     4501 gctggacagg tggcctcgac gcctgcgcgt cctcgacgag acggatgatc tgggcaagca
     4561 ccgtgtcctt tcccaccttc tcggccctga atgtgaagat actgttcgtg ttcagggttc
     4621 ccgcaacaac ctgcgcacct gctgccttga ggaccgggat cggttcgccg gtgatcatcg
     4681 attcgtccac gtaactctcg ccttctgtca ccgtgccgtc aaccgggacc ttccccccgg
     4741 gctttacccg gatcctgtcc cccacctgta catcctcaat aggcacctcc ttctccccct
     4801 cgccggtgac caggagagca gtcttcgggc ggagtcccat gagtgcccgg atggccgcag
     4861 aagtcctccc ctttgccctc gcctcgagat atctgccaag gatgaggaat gacgccagca
     4921 tgatggcggt gtcgtagaac atgaactcgt gcgtgagcac tatcccaagc gtcccgagga
     4981 cactggatac gtatgcaacc cctgtcccca tggcgtacat gacatccatg ttcagggtcc
     5041 tgttccttgc cgccatccgg gccgcacgaa agatgggata ggcaacgtag atgaataccg
     5101 gcgtggagac ggccagcatc aggtaggaga gggcatgcat ggagattggg aggggaatgt
     5161 acatgagggc aaagaggggt atgctgactg cgaatcccac catgaacctc cggaacttgt
     5221 catcgagatc ccgccgcctt gcctcctcct cggcttcgac cgtcaactgg tcggccagcc
     5281 cgaggtactg gtaccccgca tcctcgattg ccgccctcat gtcctggagc gtggtaagag
     5341 aggagttata ggtcacctgg gccttctcgg tggcgaggtt gacctgcgcc cttactacgc
     5401 ccgggagagc cgagagggcc gtctcgatcg tctccacgca ggtggcgcag accatgcccc
     5461 ccaccttcag ggtagcctgc tcgaagacga cctcgtaccc tgcctccctg acggcacgct
     5521 cgatgtccga gagcgttgca tgtgcggggt cgtactcaac ccgggcggta tcagtgccaa
     5581 aattgacttc tgcacgctct gcctttccca cggtggcaag cgacttctcg atgttcgcgg
     5641 cacacgtggc gcaatgcatc ccggatatct tcaactccag gctcttcttc tcctgacctg
     5701 tcacgggacc acctttgtcg gaagtatgaa tcacacactc ctgctgcctc atgtctctgc
     5761 acaaggtaga tgaacatttg gctccggaac aggccgggcg cgctcacacg aggctcagga
     5821 atacccagta caccacgcag tttgcaatcg tgaggggaag gcctacgcgg aagaactccc
     5881 agaacgtgag cgtataccct ctcttctccg cgttctgcac gacgatcacg ttgctcgcag
     5941 caccgaggat cgtgaggttt ccggctatcg tgcttcccgc ggcaagcgcc atcacaccct
     6001 cgatcgacag cccctgctgt gcgatgaggg gctggaagag cgcaacgaac gggacgttcg
     6061 agatgaactg gctgaaagtc acgctcgcgg cgaggatcag ggggaccgac tggagtccgg
     6121 tgaagtccag ccccgactgg aaaaatcccg actggtatac actctgcatg aggacaaaca
     6181 tcgaagcgaa gaagaccagc gtcggccagt cgattccacg cacgatggct gcgcgctgac
     6241 tgctgaagat caccaccggc agtgcccctg cgactgcgat gagcgcaagt gggatgaacg
     6301 cagggcctgt aaggacccga atgccaatga gcacgagaat gaccgcaaga gacacacggg
     6361 agagcgctgc aagcctctcg tcactgacct ggccctccgt ttcgtcgtat gcgcactcga
     6421 ttgcacccat gggggcaagt cgggtaagaa gcaggtaggc gatcacgagg ttgacgatgg
     6481 ttgggacgcc aaggtagagg agaaacgcga ggaacggctg gggcatctgg ctgtaggttg
     6541 cgacgaggag gttctgcggg ttcccgattg ggctcatcac gctcccgatg gtgaccgcaa
     6601 agcagagggc gaggagggcg gtcttcgggt gcaccccaaa ccggcaggcg tacgcgatga
     6661 cgatcggtgt cccgatgatg gcaaccgtgt cgttcatcag gagtgcggag agaaagccca
     6721 ttcccaagag gacaagaaga aggaactgcc cgctcccccg cgccctcgca aacagacgga
     6781 acgagaggga gtggaggtac ccgctcctct ccaatgcctc tcccacgaca aacatcgcaa
     6841 aaaggaagat catcacgtcg aggttgatgg agcggagcgc ctcaatgggt gcgatctgcc
     6901 ccaggaaaag gactgcgagc gctccgccga gcatcacctg ccagatgcgg aggcgaaacc
     6961 ttcccacctg gcggaccgca atcagcagga agaccgcggc caatacgatg atcggcacag
     7021 gagggacagg aaaggcgggt tccacgggaa ggtcactctg gcgatctctt ttcaaccatc
     7081 cagcgcagca ggaccccctg gtccatcctc tcgacagagt agagcgacag cccggtgaac
     7141 tgcgcttccc tgtcggcacc aggcccgtct gcgggagtag gggccatctc tcccccgatg
     7201 atcatgttcc cgatgaacgt catgtactcg tcgacaaggc caagggagaa gagcgcccct
     7261 atcagccttc ctccaccctc caccatgagg cgccggatcc ccatccggta gagctcggag
     7321 aggagggctt ccaggtccac cttcccttct cctgcccgga tcacgcaggc caggcttgag
     7381 agagcccggc acctctcgtc gtccgtatcc tggcagcatg ccaccaccct ctttcctgcg
     7441 cctttccgca ggatcgatga atccagcgga gtcctggccc tgcagtccac cactacccgc
     7501 acagggtgat ccgaaagacc ccgtgccctt ctcgcggcta tgagttctgc agacttgacg
     7561 gtgagggaag ggtcgtccgc gagcaccgtc ccgataccca ccatgatcgc gtcgctctct
     7621 gctttcagga cgtccacccg cgaaaagtcc tcctttcccg atatcttgac ctgcctccgc
     7681 tcccgggtgg agagctttcc gtcggcgctc attgcaaggt tgactacgac gtaggggcgg
     7741 agcatatgca tgaggggtgg agtgcgcaat atattgaagt tgacgatcag tataacaagg
     7801 ataacatttt cctttttttt atttaaaacg cccagattat ttttattggc attttaaatt
     7861 attatcagtg aattatatat aatataatct tataaattct aaccgttatg cctgatgatt
     7921 atatgaccat ttcagaaaac cacattcact cattttcatc ctgccaggac gttgcctcca
     7981 cctcctcctg gactgactgg agatggcata tacgccatag cgtgcgtgac attgcgaccc
     8041 ttgagtcact cctcggcata gaatttgaaa aggtggagag ggatgcgatg gaacgaacga
     8101 ttgagaagtt tcccatggcg attactccat attttctctc gctcatcgag ccggaccatt
     8161 acagaaatga cccgatattc cggcaatcgg ttccctcccc cgacgaactg ctcatcgagg
     8221 agcatgatat cgaggacccc cttcatgaag acaaggacag ccctgtgccc ggaatcaccc
     8281 acaggtatcc tgaccgcgtt ctcttccttg tgagcaatac ctgcgcgatg tattgccggc
     8341 actgcacgag aaaacggcgg gtcggagaca ttgattctgt tccttcagag caggagatcc
     8401 gaaaaggcat tgagtatatc cgtgacacgc cagcgatacg tgatgtgctc ctctcaggag
     8461 gagatcccct gatgctccct gatgactacc tgaaatggat ccttgatgag atccatgata
     8521 ttccgcacgt ggaggtcatc agaatcgggt caagaattcc cgctgtcctc ccattcaggg
     8581 tgactgactc tctcctctcc atactgagaa agcaccatcc ggtatggatg aatgtccagt
     8641 tcaaccaccc tcgtgaagtg actccgtctt cctgccgtgc acttggtaag attgcggatg
     8701 cgggtatccc tcttggaaac cagtccgttc tgctggcagg ggtgaatgac tgccccagga
     8761 tcatcagggc tctcgtgcat cacctggtga aaaacagggt gcgcccctat tacctctacc
     8821 agtgcgacct ttcagagggg ctctcccatt tccggacccc tgtcgcaaaa ggcatcgaga
     8881 ttatggagaa ccttatcggt cataccagcg gattcgcggt tcccacgtat gtcatcgatg
     8941 ctccaggtgg tggaggcaag atcccaatga tgcccaacta tacggtctca tcaggagtaa
     9001 acagggtggt gctgcgcaat tatgaagggg tgatctgcac atatcccgaa cctgatacct
     9061 accagcgccg gacctgcgat ggagactgcg tgcattgcat gctggaccac gagaacccgg
     9121 aaggaattga ggcacgacct gtgggaattg caaaactcct tgctgattat gacaatgctt
     9181 cgacgctggt tccagcagat accccaagaa tggagaggag gaatgagtga cacgatttgc
     9241 cggtatggat cgtctaccta ccagcacggg ccgctgaaca atcgcgtata cctgatgaga
     9301 gtgacctcaa aagagatccc agagatcttc gacattattg aagaacttgc ccttacatcc
     9361 aagtatgcca agatagtcgc gaaggtccct gtttcacttt caggagactt tttacagagg
     9421 aaataccaaa aagaggcatt aataccgcga ttttttcatg gcaaggagga ctgtgtcttc
     9481 atgtccaggt tcctttcgta caggaatggg aaaataccta tacccgataa ctccatgtat
     9541 gccattcgag gaagaggtcc tgttgtcaac agaaccccgg tcctccctga aggctatcgc
     9601 agtcagccct gcaaagagtc ggatgtccat gcactttcag ccctcttttc tggagtgtac
     9661 gagacatatc cattcccaat atacaacccc gatttccttt tggcgatgat gagagaggga
     9721 acacggtatc atgggatctg gtttggtgac acaattgttg ccgcatcttc ggcagaggtg
     9781 gacgccaacg agatgaatgc ggaaatgacc gattttgcca ctcttcctgc gcacagggga
     9841 ctgggactgg gaaggtacct cctttcatcg atggaacagg atctcgaggc ccgggatatc
     9901 atcaccctct ataccatagc aagggcactt tcgcgccagg tgaaccggct ttttttcggg
     9961 gccggataca gttacggagg atttctcttt cgaaacaccc agatctgcgg ctcctgtgaa
    10021 agtatgaacg tctggtacag acatctcata taaccccagt tccctgtggc accattccat
    10081 ccccagcggc gatccctccc ctggacaccc tgaatttacc agctccaccg tgggaacaat
    10141 acatgcatca aaccccttcg aatcatgata tggatttatc gggtcagaat gtcgagacca
    10201 ttccatgtat gagaatacca atagccatct tctcgatcca gccctcctga tcatcgacat
    10261 gcaaaacgac tttgtccggc ccgggacacc tctccaggtc acgggagtgt cttcaatcat
    10321 ccctaaggta agggacctcc tctcgctctt cagggagaag gatctgccgg tgttccatgt
    10381 cctccgagtt caccgggcat ccggtgtaga cgtggagatt acgcgaaggg agatctttgc
    10441 gaagaccccc tttgcagtag agggaactgc cggggccgcg gtaatatcgg aactgacccc
    10501 actgaacggg gagcatattg tgaaaaagac ccggatgagc gctttcttcc agacggacct
    10561 cgacatcatg ctgaggacac tcggggtgga cacgctcttt atcgcgggga tccagacccc
    10621 gaactgcgtc aggaccacgg tattcgacgc aatggcatac aattaccggg tcttcctggt
    10681 aagggacgcg gtcggcgcga agaccgatgc aattcacgag gcaaacgtcc ttgacatgca
    10741 gaacatcggg ataggcatcg t
//
LOCUS       DAOQYR010000077        10570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_6739_length_10570_cov_9.718117, whole genome shotgun sequence.
ACCESSION   DAOQYR010000077 DAOQYR010000000
VERSION     DAOQYR010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 10570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10570
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6739_length_10570_cov_9.718117"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="PKM87_08925"
     CDS             <1..985
                     /locus_tag="PKM87_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNQ30226.1"
                     /translation="AWGFPDGIAEGKNGYEVTIMMDVLADKPGELKCMYIMGENPMIS
                     DPDLHHVEKALKSLEFLVVQDIFMNETAELADVVLPACCYAEKDGTQTSTERRVQMWR
                     KAQDPPGQAKLDWVIIKEIASKMGYSKQFPWNSAEDIFEEIRKVTPSYAGMSYARLNK
                     PEAAHWPCPTPEHPGTPILHKEKFAHPDGLGQFFAVPYKPPAEVPDAEYPFILTTGRC
                     LWHWHTGTMTRRSKSLETEEPTGWIEINPDDAKALGIKDKEMVKATTRRGTVNVPARV
                     TKDIMKGVMFMPFHFKECAANVLTNNALDPIAKIPEFKACSVKVEKIKEGN"
     gene            986..2221
                     /locus_tag="PKM87_08930"
     CDS             986..2221
                     /locus_tag="PKM87_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HNQ30227.1"
                     /translation="MSKKGDMFYAWTPDAELAKKAECGGAVSGLLKFMLEKKIVDAVL
                     AVKRGQDIYDAVPTLVTDPAKINETAGSLHCGTLLMSKIVKKYLEGAGNNKIAITVKG
                     CDAMGLYELAKRQQVNLDNIVMIGVNCGGTVSPVLARKMIKEKFGVDPDTVTKEEIDK
                     GQFIIEYEGGHKGIKIDDLEEEGYGRRSNCRRCKMKVPRQADLACGNWGVIGDKAGKA
                     TFVEVCSDKGAGILDQAVKAGAIKTEPADQKGVEMRVKVENAMLKLGDKWREKDFASL
                     GTGRDRLKKILADTSRCIKCYQCIENCPICYCVECSTKKPHFVTPGQVPPSFMFHLIR
                     FAHIADSCVNCGQCQELCAMEIPNALFMHAQQVELEKMFGHVPGVDMKLPLLALVEER
                     EERARLSATGSDQIFDIFK"
     gene            2434..2889
                     /locus_tag="PKM87_08935"
     CDS             2434..2889
                     /locus_tag="PKM87_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNQ30228.1"
                     /translation="MTPEEYKKIISNAIDREVEAYTFYKTVADKVKDANLKKLFLELA
                     GEETKHREFLQGLLSKDVKSLKFSATKDYKVGDNIKTPALTPDMKPVDGLVVAIKKEL
                     EAMQMYTALANNSEDAAQKKMFMELASMERGHKTRLEDIYVNTAFSESW"
     gene            complement(3002..4312)
                     /locus_tag="PKM87_08940"
     CDS             complement(3002..4312)
                     /locus_tag="PKM87_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HNQ30229.1"
                     /translation="MTTNFQDLLHRALLIFAAALLAAAPVTAGAEYLGGEPILTASVA
                     GSNEFLPGSDSVMLVVIENRGIPDFKLLPQGRVTAEDQPSTAKLVKAELGAGDAPILV
                     RTDPQMAGDIPANSRVEMPFEIKILRNATGGTFTLPLTLAYSYLSFAEERGSESIAYT
                     YREAQTVVPLQVRVTPRVSIAVIDVQAGELTAGNEGYLAITIRNEGSLGGNESTARIT
                     RHEESPVIPVAGSSYIGVLGPGAQVQGRFKVQVEDHAQAAWYPLDIRVEYLDPNGDVV
                     TSEPVVIGVPVQGPIEFEITSEELTIPKGGTREVEITFRNCGPVTVRSAQARIVAVDP
                     FTSARDSASLGDLAPGQEATVSFTLSVDKTATVKEYGLESEVRYRDALNNSLISDPMK
                     VRVKVVERSGLDIVLSNPVYLSLIAVILIGTGYYFHTRRKKPSE"
     gene            complement(4454..4846)
                     /locus_tag="PKM87_08945"
     CDS             complement(4454..4846)
                     /locus_tag="PKM87_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNQ30230.1"
                     /translation="MEGGDLIEVTDLTWENTVEKSQLPVLVMFYSPTCPHCRTMEPYV
                     REFAREYSGGVLFVRIDITASQWTAERYGVRSTPTFKFFCSGRPVSDMVGAVYPALVK
                     RMIDEGLKNGRECLEHSTEINYDVSGYA"
     gene            complement(4937..5944)
                     /locus_tag="PKM87_08950"
     CDS             complement(4937..5944)
                     /locus_tag="PKM87_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HNQ30231.1"
                     /translation="MEIRKVQITGGSSFVITLPKEWAEKQNVKKNDPLGLVTQPDGTL
                     LVTRDLSESPAQRAKTFDVTSIKDPTFLFRLLIGSYIAGYTSIAITSKQRIPASIRMV
                     VREFTQMTIGPEVVEETDTSVLLKDLLNPAEMPFENTIKRMYVIVKNMYMDALAVLES
                     GNGTLAHDVVARDNDVDRLHWLLARQTNIILKNASLARKMGVAPSVVVNTYIISRIIE
                     RIGDHAVRYVEQAMKVPGGGLDPETMETIRTASAHSLSIFDRSIVSFFKADIRESHTN
                     IEQVEHLERLCQEITSRSMEHPPEVAVPLRYIAESIRRAGEYAGDISENVINMLVEDR
                     I"
     gene            6064..6933
                     /locus_tag="PKM87_08955"
     CDS             6064..6933
                     /locus_tag="PKM87_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNQ30232.1"
                     /translation="MPDSRTQVLFATSLALILAIALVAAGCTQPQGGSPKASEQKIQS
                     LSIAGSTTVLPVAQAAADAFMNTRGDADIRVSGGGSSVGIQSAGERTADIGMSSRDLK
                     PEEKDRYPDLVATVIGNDGIAVIVHSGNSVGPLTPEQVKGVYQGTYTNWKELGGPDLA
                     IVVVGRDSASGTREFFHEKVMKKEDFVPTQLEKNSNGAVKQTVMQTPGAIGYVGLGYV
                     DATTKAVPVMVSGSPVDPTVGNVLDGKYPIARPLLMVTMGEPGGLAKEYLAFLTGPSG
                     QAILAEEGFVPLV"
     gene            6979..8940
                     /gene="pstA"
                     /locus_tag="PKM87_08960"
     CDS             6979..8940
                     /gene="pstA"
                     /locus_tag="PKM87_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNQ30233.1"
                     /translation="MNEPRQSTGIFCHGRQARNKESLMGLLWFSCAIFSVAVIFFILA
                     FLFYDGFPIFVQAGLPAMLTGLVWNPTGTPPLYGTLPLIAGTLLVMLGAMAMAVPLGI
                     GSAIYIAELAHPRVKAVAKPAIELLAGIPSVVYGFFGLIVLTDWIRVTFGVPTGESWL
                     AGSILLGIMSLPTIISVSEDAIASVPREFREGSLALGATRWQTISRVVVPSAVSGITA
                     AVILGMGRAIGETMAVLMVTGNAAIIPDPIWNVLSPVRTLTGTLGIEMGEVAIGSAHY
                     HALFGVAVVLLAITLMVNLAATFILARIRQQNAPAARSCRTSRLLLNDMGSRFAWHGS
                     LKPFLLVGVISLAGLPVAGVAGFFAVALALLVLWRLKAKVSPKLAQKVAFFLLLAATA
                     TVIAILAIILFDIVSKGLPGLTWEFLTEPPRNLGRAGGIFPALVGTLYLVAGAIAIAL
                     PLGIGAAIYLTEYTREGKLTRAIRTGTDLLNGTPSIVFGLFGFSFLVLSLGFGVSLIA
                     GQVTLALMVLPTIIRTTEEALKNVPHALREGSLALGATRWQTISRVVIPPAVPGIITG
                     AILSIGRAAGETAPILFTAAVFSQRFLPSSLFDPVMALPYHLYILATNVPGADANKYA
                     TALVLLILVVLIYSAAILVRTHSQKTTRG"
     gene            8946..9707
                     /gene="pstB"
                     /locus_tag="PKM87_08965"
     CDS             8946..9707
                     /gene="pstB"
                     /locus_tag="PKM87_08965"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNQ30234.1"
                     /translation="MTTQSIISTQGLTLWYGETRALKGVTMEVADQKVTALIGPSGCG
                     KSTLIRCFNRMNDLVCDCRIEGSVDFHGNNIYGNGADPVDIRRRIGMVFQKPNPFPKS
                     IYENVAYGPRVHGVTDRARLDQVVEESLRKAALWDEVRDRLSHSAMCLSGGQQQRLCI
                     ARTLAVGPEVILMDEPCSALDPIATAKIEALIGELKQQYCVIIVTHNMQQASRVSDFT
                     GFMYLGEMVEFGETAQIFESPREELTENYITGRFG"
     gene            9719..10375
                     /gene="phoU"
                     /locus_tag="PKM87_08970"
     CDS             9719..10375
                     /gene="phoU"
                     /locus_tag="PKM87_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNQ30235.1"
                     /translation="MTDKFHDEMAGMQQKVLGMGLFAEGMLRDAVEALRTQDISLAGD
                     VKARKGALSSQNDILEEEVYRMIALYQPVARDMRAVACALKVIASAERIGRYGKDIAN
                     VVIATADSRGKDLASGLAIPHMAGLVLSMIDDSLRAYETGDLEFIRHHSERDDVVDAL
                     RHTIFREGITYMMEEPRTITRCMNYLMVARYLERCADHSCKIAEKVHYMVTGERIEIK
                     "
BASE COUNT         2173 a         3260 c         3083 g         2054 t
ORIGIN      
        1 cgcatggggc ttccctgacg gcatcgccga gggcaagaac gggtacgaag tgaccatcat
       61 gatggacgtg ctcgcagaca agcccggtga gctcaagtgc atgtacatta tgggcgagaa
      121 cccgatgatc tcagaccccg acctccacca cgtggaaaag gccctgaaga gccttgagtt
      181 cctcgtcgtg caggacatat tcatgaacga gaccgcggag cttgcagacg ttgtcctgcc
      241 tgcctgctgc tacgccgaga aggacggcac ccagacctct accgagcgcc gcgtccagat
      301 gtggcggaaa gcccaggacc cgccgggcca ggcaaagctc gactgggtga tcatcaagga
      361 gatcgcctcc aagatgggct attcaaagca gttcccctgg aacagcgccg aagacatctt
      421 cgaggagata cgtaaagtca ccccgtccta cgcaggcatg agctacgcga ggctgaacaa
      481 gcccgaggct gcccactggc cctgccccac tcccgagcac cccgggaccc ctattctcca
      541 caaggagaag ttcgcccacc ccgatggact gggccagttc tttgccgtgc cctacaagcc
      601 cccggcagag gtccccgacg ccgagtaccc gttcatactc acgactggcc gctgtctctg
      661 gcactggcat accggcacga tgacccgaag gtcaaagtcc cttgagactg aagagcccac
      721 gggatggatc gagatcaacc ccgacgatgc aaaggcactc ggcatcaagg acaaggagat
      781 ggtcaaggcc acgacgaggc gcgggactgt caatgtcccc gcccgtgtca cgaaggacat
      841 catgaagggc gtcatgttca tgccgttcca cttcaaggag tgcgccgcaa acgtccttac
      901 caacaacgca ctcgacccca tcgccaagat cccggagttc aaggcctgtt ctgtcaaggt
      961 cgagaagatc aaggagggga actagatgtc caagaaaggt gacatgttct acgcgtggac
     1021 cccggatgcc gagcttgcca agaaggcaga gtgcggcggc gcagtctcag ggctgctgaa
     1081 gttcatgctc gagaagaaga tcgttgacgc agtcctcgcg gtcaagaggg ggcaggacat
     1141 ctacgatgcc gtgccgacgc ttgtgaccga ccctgccaag atcaacgaga cggccgggtc
     1201 gctccactgc ggcaccctgc tcatgtcgaa gatcgtgaag aagtaccttg agggagcggg
     1261 caacaataag atcgctatca ccgtcaaggg atgcgacgcc atgggcctgt acgagcttgc
     1321 caagcggcag caggtgaacc ttgacaacat cgtcatgatc ggggtcaact gcggagggac
     1381 cgtcagcccc gtccttgccc ggaagatgat caaggagaag ttcggggtcg acccggacac
     1441 ggttaccaag gaagagatcg acaagggcca gttcatcatc gagtacgagg gcggccacaa
     1501 gggcatcaag atcgatgacc tcgaggagga gggctacgga cgccgctcca actgccgccg
     1561 ctgcaagatg aaagtcccgc gccaggcaga ccttgcctgc gggaactggg gagtcatcgg
     1621 cgacaaggcc gggaaggcca cgtttgtcga ggtctgcagc gacaagggtg caggcatcct
     1681 cgaccaggcc gtcaaggcag gggccatcaa gaccgagccc gccgaccaaa agggcgtcga
     1741 gatgagggtc aaggtcgaga acgccatgct caagctcggc gacaagtggc gggagaagga
     1801 ctttgcgtca ctcgggaccg gccgcgaccg cctcaagaag atcctcgccg atacctcccg
     1861 gtgcatcaag tgctaccagt gtatcgagaa ctgcccgatc tgctactgcg tcgagtgcag
     1921 caccaagaag ccgcacttcg tgacccccgg gcaggtcccc ccgagcttca tgttccacct
     1981 gatccggttc gcccatatcg ccgactcctg cgtcaactgc gggcagtgcc aggaactctg
     2041 cgcaatggag atcccgaatg cgctgttcat gcacgcccag caggtcgagc tcgagaagat
     2101 gttcggacac gtccccggag tcgacatgaa actcccattg cttgcactcg tcgaggaacg
     2161 cgaggagcga gcccggcttt ccgccacggg gagcgaccag atcttcgata tcttcaagta
     2221 aatccactca ttcttttttt tagccgaaat ttgagacgct cacattttcc accggcctca
     2281 tttcagcgca attcgtcgat gtaaattgat tgtgattaat tattggtttt tggaaattag
     2341 gactgcattt ttatgaattc acgaaaaggt gggcaagaaa cgcgctctta tatagggaca
     2401 tggagtatcc tagtggtgga ggatctgatt cgaatgactc cagaagagta caaaaaaatc
     2461 atctcaaatg ccatcgaccg cgaagtggaa gcctatacct tctacaagac cgtcgcagac
     2521 aaggtaaagg acgcaaacct caagaagctc ttcctcgagc tcgcggggga agagaccaag
     2581 caccgcgagt tcctgcaggg gctcctctca aaggatgtaa agtccctcaa gttctctgcc
     2641 acaaaggatt acaaggtggg agacaacatc aagaccccgg cgctcacccc tgacatgaag
     2701 ccggtcgacg ggctcgtcgt ggccatcaag aaggagctcg aggcgatgca gatgtacacg
     2761 gcgcttgcaa acaacagcga ggacgctgcc cagaagaaga tgttcatgga gcttgccagc
     2821 atggagcggg ggcacaagac ccgtctcgag gacatctacg tcaatacggc attctccgag
     2881 agctggtaag aacaggtata cccattccct cttttttttc agagcactgc tgcgcacgct
     2941 gccaggatat gattcactgc ttccggtgac agccaaaaaa ttcagggaga tttcaagaat
     3001 actattccga gggtttcttc cgccgggtgt ggaagtagta gcctgtcccg atcagtatga
     3061 ccgcgatgag acttaagtac acggggtttg agagaacgat atcaagtcct gacctctcca
     3121 ccacctttac cctcactttc atgggatcgg agatgaggct gttgttgagg gcatcccggt
     3181 aacgcacctc ggattcgaga ccatactcct tcaccgtcgc ggtcttgtcg accgagaggg
     3241 tgaaggacac cgttgcctcc tggcccggcg ccaggtcccc aagggacgca gagtctctcg
     3301 ccgaggtgaa gggatcgacc gccacgatgc gggcctgggc gctccggaca gtcacggggc
     3361 cgcaattcct gaatgtgatc tcaacctccc gtgtcccgcc cttcgggatg gtaagctctt
     3421 ctgacgtgat ctcgaactca atggggccct gcactggcac tccaataacg acgggctcgg
     3481 aggtgaccac atctccgttg gggtcaaggt actcgacgcg gatgtcaagg gggtaccacg
     3541 ccgcctgggc atggtcctcg acttgcacct tgaacctccc ctggacttgc gcccccggac
     3601 caagcacccc gatatacgag ctgcctgcca ccgggatcac cgggctctcc tcgtgcctcg
     3661 tgatcctggc ggtcgattcg ttcccgccaa gggacccctc gttcctgatg gtgatggcga
     3721 gatatccctc gttcccggca gtcagttccc ctgcctggac gtcgatgacc gcgatgctga
     3781 cgcggggtgt gaccctcacc tggagcggca cgacggtttg agcctccctg tacgtatagg
     3841 ctatcgactc tgatccccgc tcttccgcga aggagaggta tgaatacgcg agggtgagcg
     3901 ggagcgtaaa tgtcccgccc gtcgcattcc ggagtatctt gatctcgaag ggcatctcga
     3961 cccgtgaatt cgcggggatg tctcccgcca tctgcgggtc ggtccgcacc aggatcggcg
     4021 catcacctgc gccaagttcg gccttcacca gcttggcagt ggacggctgg tcttcggcag
     4081 tgaccctccc ctgtgggagg agcttgaaat ccgggatccc gcggttctca atgacgacca
     4141 gcatgactga atcgcttccc ggaaggaact cgtttgaccc cgcgacggac gcggtgagga
     4201 tgggctcacc acccaggtat tcggctcctg ccgtgaccgg cgcggcggca agaagcgccg
     4261 ctgcgaagat gagaagtgct cgatggagga gatcctggaa atttgttgtc attttaataa
     4321 caacattttg agaattgcgt tatttatatc tattgagatt gcctggatgg cggcagcgga
     4381 taccgcattt cccgacagtt cccatgcgcc tgccgcgcac ccgggacccc atccctgggc
     4441 agtcggatta atttcacgcg tatcctgaca cgtcgtaatt gatctcggtt gagtgctcaa
     4501 ggcactcgcg gccattcttc agaccttcgt ctatcattct cttcacgagt gcaggataga
     4561 ccgcgcccac catgtccgag acgggccgtc cgctgcagaa gaacttgaag gtcggcgtgc
     4621 tccggacacc ataccgctct gccgtccact ggcttgcggt gatgtcaatc ctcacaaaga
     4681 ggaccccccc ggaatactcc cgcgcaaact ccctcacgta cggctccatt gtcctgcagt
     4741 gggggcaggt ggggctgtag aacatgacca gcaccggaag ctggctcttc tcaacggtat
     4801 tttcccaggt gagatcggtc acctcgatca ggtctccccc ttccattcct tactcctccc
     4861 gggtgtgagg ttgcaggcgt ggggtattat aggttggccc ttgcacgaga catggcagcg
     4921 gggaggcacg ggccagttat atccggtcct ccacgagcat gttgatcacg ttctccgaga
     4981 tatcccccgc atactccccg gcacgcctga tactctcggc gatgtacctg aggggcactg
     5041 caacctcagg cggatgctcc atcgagcgcg aggtgatctc ctggcagagc cgctcaaggt
     5101 gctccacctg ctcgatattg gtatgcgatt cccggatgtc agccttgaag aacgagacga
     5161 tgctccgatc gaaaatggag agggagtggg cggaggccgt tcgtatcgtc tccatcgttt
     5221 cagggtcgag tccgcctcct ggcactttca tcgcctgctc gacgtacctg accgcgtggt
     5281 cccctatccg ctcgatgatc ctgctgatta tatacgtgtt cacgacgacg gacggcgcca
     5341 ctcccatctt cctggcgagg cttgcattct tgaggatgat gttcgtctgc ctggccaaga
     5401 gccagtgaag gcggtccacg tcgttgtccc ttgccaccac gtcgtgcgca agggtgccgt
     5461 tgccggactc gagcactgcc agggcgtcca tgtacatgtt cttgacgatc acgtacatgc
     5521 gcttgatggt gttctcaaat ggcatctcag ccgggttgag caggtctttc aagaggacgg
     5581 aggtatcggt ctcctccacc acctcgggcc caattgtcat ctgggtgaac tccctcacca
     5641 ccatccggat cgaggcagga atgcgctgct tcgaggtgat ggcaatcgac gtgtagcctg
     5701 caatgtagga cccgatcagg aggcggaaga ggaacgtggg gtccttgatg gacgtgacgt
     5761 caaaggtctt ggcacgctgt gccggggact cggagaggtc ccgcgtcacg agcagcgtgc
     5821 cgtcaggctg ggtcacgagg ccaagcgggt cgttcttctt cacgttctgc ttctctgccc
     5881 attcctttgg cagagtgatc acaaacgacg accctcccgt tatctgcacc ttccggatct
     5941 ccactcctct cctcctgccc tttttctata ctactctatt ccccacaata tattatatat
     6001 atacatatag tctccatcga aaaaaggtat accggaaccc cccgaatttt ttcttcgaac
     6061 accatgcctg attcacgcac acaggtgctt tttgccacca gcctcgcgtt gatcctcgcg
     6121 attgcgcttg tcgcggccgg ctgcacgcag ccgcaggggg ggtcgccgaa ggcctctgaa
     6181 cagaagatcc agtccctctc cattgcgggt tctaccaccg tgctcccggt tgcccaggct
     6241 gcggccgacg cgttcatgaa cacccgcggc gacgcggaca tccgggtcag cgggggcggc
     6301 tccagtgtcg gcatccagtc cgcaggcgag cgtaccgcgg acatcggcat gtcttcgcga
     6361 gacctgaaac ctgaagagaa ggacaggtac ccggacctcg ttgccactgt gatcggaaac
     6421 gacggcatcg cggtgatcgt ccactccggc aactccgtcg ggccactcac cccagaacag
     6481 gtgaagggag tgtaccaggg gacctacacc aactggaagg aactcggcgg acctgacctt
     6541 gcgatcgtgg tggtggggag ggacagcgcc tcagggacaa gggagttctt ccatgaaaaa
     6601 gtgatgaaga aggaggactt cgttcccacg caactcgaga agaactcgaa cggtgccgtg
     6661 aagcagacgg tcatgcagac gcccggggca atcgggtatg tcgggctcgg ctacgtggac
     6721 gcgaccacaa aagcggtccc ggtcatggtc tccggatccc cggtcgatcc gactgttggg
     6781 aacgtgctcg acggaaagta tccgatcgca aggcctctcc tgatggtcac gatgggagaa
     6841 cctgggggac tcgcaaagga ataccttgca ttcctgacag ggccttcggg gcaggcgatc
     6901 ctcgcagaag aggggttcgt gcccctggta tagtcccccg gcatcccaca tgtccttcct
     6961 gacagagacc agaatcccat gaatgaaccc aggcagtcca ccgggatctt ctgccacggg
     7021 aggcaggcac gaaacaagga gtccctcatg gggctcctct ggttctcctg cgcaatcttc
     7081 tcagtcgccg tcatcttctt catcctcgcg ttcctgttct acgacgggtt ccccatcttc
     7141 gtccaggcag gtttgcccgc gatgctcacc ggcctcgtgt ggaaccctac cgggacccct
     7201 cccctgtacg ggaccctccc cctgatcgcc ggcacgctgc tcgtcatgct cggtgcaatg
     7261 gcaatggcag tcccgctcgg catcgggagt gcgatctaca tcgcggagct cgcgcatccc
     7321 cgcgtgaaag ccgtcgcaaa gcctgccatc gagctcctgg cagggatccc ttccgtggtg
     7381 tacgggttct tcgggctgat tgtcctcacc gactggatac gggtcacctt cggcgttccc
     7441 accggcgagt cgtggctcgc ggggtcaata ctcctcggca tcatgtcgct tcccaccatc
     7501 atcagcgtct cggaggacgc catcgcctcg gtcccccgcg agttccgtga gggcagcctc
     7561 gcgctcgggg ccacgagatg gcagaccatc tcccgggtcg tcgtcccctc cgcggtctcg
     7621 ggaattacag ccgcagtcat cctcggcatg gggagggcga tcggggagac catggcggtt
     7681 ctgatggtga cagggaacgc cgccatcatc cccgacccga tctggaatgt cctctccccg
     7741 gtccggaccc tcaccggcac cctcgggatc gagatgggtg aggtcgcaat cgggagcgcg
     7801 cactaccacg cgctcttcgg ggtcgcggtg gtgttgctcg caatcaccct catggtgaac
     7861 ctcgccgcga ccttcatcct cgcgaggatt cggcagcaga acgcaccggc tgcaaggtcc
     7921 tgcaggacat cccggctcct gctgaatgat atgggatcgc ggttcgcatg gcacgggtcc
     7981 ctcaaaccat tcctcctggt aggtgtcatc tcgcttgcgg ggctccccgt cgcgggcgtc
     8041 gcgggctttt ttgccgttgc gctcgcactc ctcgttctct ggcgcctgaa ggcgaaagtc
     8101 tccccgaaac ttgcgcagaa ggtcgccttt ttccttctcc ttgccgccac ggcgacggtg
     8161 attgcgattc tcgccatcat cctcttcgat atcgtcagca agggccttcc gggcctgaca
     8221 tgggagttcc tcacagagcc cccgaggaac ctcgggcgtg ccggcggcat cttccctgca
     8281 ctggtgggaa ccctctacct cgtggcaggg gcgatcgcga ttgcgctccc gcttgggatc
     8341 ggcgcggcca tctacctgac cgagtatacc cgggagggga aactcacgcg ggcgatccgc
     8401 acggggaccg acctcctgaa cggcacccct tccatcgtct ttgggctctt cgggttctcg
     8461 ttcctggtcc tctccctcgg attcggggtc tcactgattg caggacaggt gacgcttgcg
     8521 ctgatggtgc ttccgaccat catccggacc acggaggagg ccttgaaaaa cgttccccac
     8581 gcgctcaggg agggaagcct tgcgctgggg gcaacgaggt ggcagacgat ctcgcgggtg
     8641 gtgatccccc ccgcggtgcc cgggattatc accggcgcca tcctctccat cgggagggct
     8701 gcgggcgaga ccgcccccat cctcttcacg gcggcggtct tctcccagag gttcctcccc
     8761 tcctcgctct ttgaccccgt catggcgctc ccctaccacc tctacatcct cgccacgaac
     8821 gttcccggcg cggatgcgaa caagtatgcg actgcactgg tgctcctgat cctggtggtc
     8881 ctgatctact cggcggccat cctggtacgg acccattccc aaaagaccac acgagggtaa
     8941 taccattgac aacccagtcc atcatctcaa cgcagggcct caccctgtgg tacggagaga
     9001 cacgcgccct gaaaggcgtc acgatggagg tagcggacca gaaggtgact gccctcatcg
     9061 gcccgtcagg gtgcggcaag tccaccctga tccgctgctt caaccgcatg aacgacctcg
     9121 tttgcgactg ccggatagag gggtcggtcg acttccacgg gaataacatc tacgggaacg
     9181 gcgcagaccc cgtcgatatc cgccgccgga tcgggatggt cttccagaaa ccgaatccct
     9241 tcccgaagtc catctacgag aacgtggcct acgggccgag ggtccacggc gtcaccgatc
     9301 gggcgcggct cgaccaggtc gtcgaggaga gcctgcggaa ggccgcgctc tgggacgagg
     9361 tgagggaccg gctctcccac tcggcgatgt gcctctcggg cggccagcag cagcggctct
     9421 gcatcgcaag gacgctcgcg gtcgggcccg aggtcatcct gatggacgag ccgtgctccg
     9481 cgctcgaccc gatcgcgacg gcaaagatag aggcgctcat cggagaattg aagcaacagt
     9541 actgcgtgat catcgtgacg cacaacatgc agcaggcatc gcgggtgagc gatttcaccg
     9601 ggttcatgta cctcggggag atggtcgagt tcggggagac cgcccagata ttcgaaagcc
     9661 caagggaaga actgacagaa aactacatta cagggaggtt tgggtaggtg gtgtgcacat
     9721 gaccgacaag ttccacgacg agatggctgg aatgcagcag aaggtcctcg ggatggggct
     9781 cttcgccgaa gggatgctcc gcgacgcagt ggaggcactg aggacacagg acatctcact
     9841 tgcaggcgat gtcaaagccc ggaaaggggc actctcctcg cagaacgata tcctcgagga
     9901 ggaagtttat cggatgatcg cgctctacca gccggtggca agggacatgc gggccgtcgc
     9961 atgcgccctg aaagtcatcg cctctgcaga acggatcggc cgctatggaa aagacatcgc
    10021 aaatgtcgtc atcgccaccg ccgactcccg cgggaaagac ctggcaagcg gccttgcaat
    10081 cccccacatg gcaggcctcg tcctctcgat gatagacgat tccctgcggg catacgagac
    10141 cggcgacctg gaattcatcc gccaccactc tgaacgcgat gacgtggtgg acgcgctccg
    10201 ccataccatc ttccgcgagg ggatcactta catgatggag gagccccgaa ccatcacccg
    10261 atgcatgaac tacctgatgg tcgcccgtta cctggagcgg tgcgcagacc actcctgcaa
    10321 gatcgccgag aaggtccatt atatggtgac gggagaacga atagagataa agtgatgcgt
    10381 gtggtgcagc gctcccggcg cgcctgcgaa tcgggacctt ctgcttcatt ttttcacgca
    10441 catcgcggca gtccgaccac aaacatttaa accaagcctg ccacaccatc tgagtaatac
    10501 ctcttgtgct gcagaccctg cggctcgtgg ttccccatgc cagaaccaga gaacgagaac
    10561 gacgatgacc
//
LOCUS       DAOQYR010000078        10301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_6961_length_10301_cov_8.357408, whole genome shotgun sequence.
ACCESSION   DAOQYR010000078 DAOQYR010000000
VERSION     DAOQYR010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 10301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10301
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6961_length_10301_cov_8.357408"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..569)
                     /locus_tag="PKM87_08975"
     CDS             complement(12..569)
                     /locus_tag="PKM87_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNQ30236.1"
                     /translation="MNLRRQHALSIAYTGAFTAFLAAGSWISIPFVPVPFTLQTLFLF
                     LAAGLMGRYSVAPVSLYLLMGAMNFPVFHSGTSGIGVFLGPTGGYLLGFIPAALLSGL
                     GYERKLRLARYGGMAAGLCALYACGVAWLSLSTGLSLEMAILLGAVPFLPGDILKAVV
                     ASLATDRIARLKSGMHAGITGEKPG"
     gene            complement(609..1586)
                     /locus_tag="PKM87_08980"
     CDS             complement(609..1586)
                     /locus_tag="PKM87_08980"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNQ30237.1"
                     /translation="MYEAAFRVLEVLETADGPVSGETISNTLGISRSAVWKHIKELNN
                     MGYEITASHREGYRLKKSSNRLLPYEVHKKLRTAFMGKQMRYFESTTSTNWVGRQMAE
                     EKEPSSLHGMVIIAEQQTGGIGRLGRSWVSPRGGIWATVVIRPKIPIERVFMITMAGA
                     IAIARALRKEYDLGALIKWPNDIYIGDSKVAGLLLELSAEADQIHYALLGLGVDVNVS
                     PADLSGLPHAVTSVSLEVGHDVDRPALLARILREFESRYLLLEAGEYESIVREWKSLS
                     CTLEHRVRVHTLKTSIEGEAIDIDEYGALLVRKDNGKVERVIAGDCTQQ"
     gene            complement(1591..2013)
                     /locus_tag="PKM87_08985"
     CDS             complement(1591..2013)
                     /locus_tag="PKM87_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2111 domain-containing protein"
                     /protein_id="HNQ30238.1"
                     /translation="MNRFILSAESGPEDLEPLGLALHELLNRLPITARSLERPGIRIE
                     DGRVIDANYSGPVLEQVLQENRILKVTPSRGAYKGVPVVVGPIRDSAGAAITAIGIVD
                     ITGIFDLATLMDHQSEILKQVCGKDPCPLPGEQVVAKR"
     gene            complement(2048..2623)
                     /locus_tag="PKM87_08990"
     CDS             complement(2048..2623)
                     /locus_tag="PKM87_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30239.1"
                     /translation="MNRHCALLWDEKTVFSRFIEECGLTCEHVTPHLLAAPFYRGCYS
                     TLIIPAGFANPSYSRLLPALRASSGRIRRYMEAGGKIIVFGAGIDRADAYDWLPFDVT
                     YCHQHRGGGLECISTHRCASLFAGYDPESIESDGYFSDHHGEVIAKMGGSAVLIRSDV
                     GNGEVIVTTIHEYPSRAFLQEFCKGSLETFL"
     gene            complement(2620..>2784)
                     /locus_tag="PKM87_08995"
     CDS             complement(2620..>2784)
                     /locus_tag="PKM87_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNQ30240.1"
                     /translation="LGQEGIIVRAPSDAAIADEAPEVYKPSMEVVRVVHEVGISRLVA
                     RLEPLGVIKG"
     assembly_gap    2785..2884
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2885..3786)
                     /locus_tag="PKM87_09000"
     CDS             complement(<2885..3786)
                     /locus_tag="PKM87_09000"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNQ30241.1"
                     /translation="MLPGIQRVREFEWQVPVGYVPGMRVPGVFFLSETLAGTLEEGAV
                     QQLANVATMPGIVARSLAMPDIHWGYGFPIGGVAAFSLDEGVISPGGVGFDINCGVRL
                     LATPLREEDLVKKRELVERLFKAVPTGVGGKSTLRLSGHELDRMLLEGSSWAVGAGFG
                     IDEDVRMCEQQGCMKEAETAAVSQKARQRGMPQSGTLGSGNHFLEIQVVKEVYQPEVA
                     RAFGISAGQVCIMIHCGSRGLGHQVCTDHLRTLEAATRKYGITLPDRQLACAPIDSPE
                     GKAYFGAMAASANYAWANRQVIMH"
     gene            complement(3793..4200)
                     /locus_tag="PKM87_09005"
     CDS             complement(3793..4200)
                     /locus_tag="PKM87_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HNQ30242.1"
                     /translation="MSFEELEHTADARVRVRAGTLEGLFSEAGRALMQVMYGRPGRDG
                     LSRSVFVQGDDLESLMHAFLSEILFITEVDGLVISGAEVRVGEGIVEGVVRGEPFSRD
                     IHNAGRGVKGISYSGLSIVRDKESYILDVIFDV"
     gene            complement(4190..4486)
                     /locus_tag="PKM87_09010"
     CDS             complement(4190..4486)
                     /locus_tag="PKM87_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30243.1"
                     /translation="MYVRPNTLTPIEDIVVNEDVADYIRRRGCDFRICTSCGGPLLLP
                     VSMKPPKATDLQVRTGDHTIFISIHQARYLQSIHRGMLPMFLDQIEDQSPGHEF"
     gene            complement(4538..4852)
                     /locus_tag="PKM87_09015"
     CDS             complement(4538..4852)
                     /locus_tag="PKM87_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2551 domain-containing protein"
                     /protein_id="HNQ30244.1"
                     /translation="MRSPHEIKAVIEGRLRMYLSRDHTGIRRAMLQLFLKIKSLTIAK
                     ICEYLNRRFNISYHSVAAMVGIIASRIGILHVMKNREGTHSVYQLKEQYTDLVAKIVG
                     TT"
     gene            5049..5903
                     /locus_tag="PKM87_09020"
     CDS             5049..5903
                     /locus_tag="PKM87_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNQ30245.1"
                     /translation="MGLIGYDVNKYSPRQMVMIPMVILILSLAILGYNMATIGMPVRP
                     GIDFSGGTAVTLFTTDTPSQLEQKFQGYPLLSVGEGVNNGKYLKFGPMDDTSVQSLAA
                     LIGAEYPDAKIDQIGASFGKTLQQQALVALIISFIGMALVVFLAFRVFVPAIAVIFCA
                     FTDITMTAAGMSLVGIDLSLPTVAALLMLIGYSVDSDILLTMRVLKRQGKLEEKLSGA
                     FQTGIIMTTTTIAAVAAMWVVAFAGQITVIWEIATVLLIGLVLDMMNTWLTNAGIIKW
                     FVTRRGGK"
     gene            5900..7354
                     /locus_tag="PKM87_09025"
     CDS             5900..7354
                     /locus_tag="PKM87_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071806 - protein transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecD"
                     /protein_id="HNQ30246.1"
                     /translation="MSDSKLMACLKHWQVALMVILLVASVIAIAPRFEDGKFTTNLQY
                     GLDLQEGTWLQMEFKAEVVGFQTDRSVEDFVTDLEKQLDAEIYLVDEGKLEIRSYYPE
                     ETLETIFASAGGKLTSYEQGVTRETADDVKRILENKLNALGTKDARVNPLTGLSGVTH
                     YIRVELAGVSLSQAKEIVGKQGKFEIRIHTTGNQTEHVLYGDTITSVGVPSHEPPGSA
                     VWGVSFTLSEDGAKKFQQGAIATGAVSDPDAHYLDMVLDEKVVYSAPLSGDLAAKLAI
                     EPIRQLYASTGTGPAGLEAAKNLEIHLRAGALPVDVTVAGSGYVPAGLSDYFRMMCVI
                     AFIAAAIAVGVVIYYRYREASIVLPMIGTNLTEIVILLGVATLIQQLDLAAIAALIAV
                     MGTGIDQLVIITDEILHEGKVPSPTLYKKRLARALGIILVSAFTVIIAMIPLALMDLS
                     SLRGFALITIVGVVIGVTVTRPAYGEIIKEILSK"
     gene            7409..7681
                     /gene="trxA"
                     /locus_tag="PKM87_09030"
     CDS             7409..7681
                     /gene="trxA"
                     /locus_tag="PKM87_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNQ30247.1"
                     /translation="MSRPVLLDFYADWCGPCRRQGPILEELKKKMGDRVEIKKIDVDQ
                     HMEMANKYGIRVVPTLIIEKDGKVMHSLEGVTSAETLESMLSRLVD"
     gene            7682..8332
                     /gene="npdG"
                     /locus_tag="PKM87_09035"
     CDS             7682..8332
                     /gene="npdG"
                     /locus_tag="PKM87_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HNQ30248.1"
                     /translation="MKVGIVGGTGDIGEGIAMRLSSRHDVVIGSREEEKAVQSSECCL
                     ETLTKKGLPCSVRGHTNQEAVDRSDVVILAIPFRHVEPTLSSLKGFSGKIVVSPVNPM
                     EKRDYFVCVPPPEGSAALMIRRLLPPDARLCTAFNTVAANKWRALDEELELAIPVCGD
                     DAEAKAVVMDLINSVPRVRAYNAGPLAASCMVECLTPLLLNIARFNKMRDVGIQFK"
     gene            8373..8912
                     /locus_tag="PKM87_09040"
     CDS             8373..8912
                     /locus_tag="PKM87_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HNQ30249.1"
                     /translation="MNHAAEFQRIGKRLREEGLVSANFGNMSVRKGDGLLITRNGAFL
                     DSPGELVYVAMAGAIPQEASSECKLHREVYRITPHHAVVHAHPPHAVATSLHRDRIVP
                     VDSEGVMFCPSVPVVTGLPGSMEMAENVASALKDHKVVIVRGHGTVCGAGSLEEGYVL
                     TSIVEHSCRVLLLSRGGCD"
     gene            8998..9339
                     /locus_tag="PKM87_09045"
     CDS             8998..9339
                     /locus_tag="PKM87_09045"
                     /inference="COORDINATES: protein motif:HMM:NF021558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HNQ30250.1"
                     /translation="MTGIEEKISRLPPELQKEVEDYIDFLVRRHAPARAQEQVPGFLS
                     PPPAPAPSKPIILADEIPFRTEPEILPGYKDLGEFSEPEARSIPVKEPSVRTRRPKDA
                     EPGRQLLDWID"
BASE COUNT         2222 a         3002 c         2855 g         2122 t
ORIGIN      
        1 gatcatcgcg cctaccctgg tttttcgccc gtgatccccg catgcatccc tgacttcagc
       61 cgtgcaatcc gatcggttgc cagggatgca accaccgcct tcaggatatc gccgggaaga
      121 aaggggacgg ccccgaggag tattgccatc tcaagcgaca gcccggtgga gagcgagagc
      181 catgccactc cacaggcgta gagcgcacag aggcctgccg ccatcccccc gtaccgggca
      241 agccgcaatt ttctctcgta cccgagaccc gagagaaggg ccgcgggaat gaaccccaac
      301 aggtaccccc ctgtcggccc gaggaacacc cctatccctg acgtcccgct gtgaaatacc
      361 gggaaattca tggcccccat caggaggtaa agagagaccg gggccaccga gtatctcccc
      421 atgagccctg ccgcgaggaa gaggaagagg gtctggaggg tgaatgggac cgggacaaag
      481 gggatggaga tccatgaacc tgccgcaagg aatgcagtga acgctcccgt ataggcgata
      541 gagagtgcat gctgccttcg gaggttcatc gtcaacctga gattaattcg tggttgacga
      601 gaagatagtt actgctgagt gcagtcgccg gcgatcacgc gctcgacttt tccgttgtcc
      661 ttgcggacca ggagtgcgcc atactcgtcg atatcgatcg cctcgccctc gatgctggtc
      721 ttgagagtgt gaacgcggac gcggtgctcc agcgtgcagg agagactctt ccattccctg
      781 acgatcgact cgtattcgcc cgcctccagg agcaggtagc ggctctcgaa ctcccttaaa
      841 atacgggcaa ggagtgcggg gcggtccacg tcatgtccta cttcaaggct gaccgatgtc
      901 accgcgtggg gcagcccgga gaggtccgcg ggcgatacat tcacgtccac cccgaggcca
      961 agcagtgcgt aatggatctg gtccgcctct gcggagagct cgaggaggag gcctgccacc
     1021 ttgctgtccc cgatatagat atcgtttggc cacttgatga gggccccgag gtcgtactcc
     1081 tttcgcagcg ccctggcgat cgcaatcgct cccgccatgg tgatcatgaa gaccctctcg
     1141 atgggaatct ttggcctgat cacgacagtg gcccagatcc cgccgcgtgg ggagacccat
     1201 gacctcccca gtctcccgat gcccccggtc tgctgctcag cgatgatcac catcccgtgc
     1261 agcgacgatg gttccttctc ttccgccatc tgcctgccta cccagttggt cgaagtggtg
     1321 ctctcgaagt agcgcatctg cttgcccatg aaggcggtcc ggagtttctt gtggacctcg
     1381 taggggagca gacggttgct gctcttcttg agccggtacc cttcccggtg ggacgcggtg
     1441 atctcgtagc ccatgttgtt cagctccttg atgtgcttcc acaccgcaga gcgtgatatc
     1501 cccagggtat tgctgattgt ttcgccggaa acaggtccat ccgctgtctc caggacttca
     1561 aggaccctga acgctgcctc gtacatgatg ctaccttttt gccacgacct gctctccagg
     1621 aagcgggcaa ggatccttcc cgcagacctg cttgagaatt tcagactggt gatccatgag
     1681 ggtagcgaga tcgaatatac ccgtgatgtc cacgatccct atggctgtga tggcagctcc
     1741 cgccgaatcc cggatgggac cgaccacgac cgggacaccc ttgtaggctc ccctggaagg
     1801 ggttaccttt aatatgcggt tctcctggag gacctgttcg aggacaggcc ctgaataatt
     1861 cgcatcgatc acccgcccat cctcgatcct gatgcctggc cgctcgaggg aacgcgctgt
     1921 aatgggaagc ctgttcagga gttcatggag tgccagtccc agtggttcga gatcctccgg
     1981 cccggattct gctgagagga taaagcggtt catacgtgga tcactacact aaatccctga
     2041 tttccagtca caagaatgtt tccaatgaac ccttgcagaa ttcctggaga aatgcccgtg
     2101 aaggatattc atggatggtg gtaacaatta cctcgccatt gcccacgtcg ctcctgatca
     2161 ggactgcact ccctcccatc ttcgcgatta cctcaccgtg atggtctgaa aaatacccgt
     2221 cactctctat ggattccggg tcgtatcctg caaagagcga tgcgcagcgg tgcgttgaga
     2281 tgcattcgag cccgccgccc cggtgctggt ggcagtaggt gacgtcgaac ggcagccagt
     2341 cgtaggcatc cgcccggtcg atccccgcac caaagacaat gatcttccct cctgcttcca
     2401 tatacctcct gatgcggccc gaagaggcac gcaacgctgg caggagccgg gaataggagg
     2461 ggtttgcaaa gcccgcgggg atgatcaggg tggaatagca ccccctgtaa aagggtgccg
     2521 cgaggagatg gggggtcacg tgttcgcagg tgaggccgca ttcctcgatg aaccgggaaa
     2581 aaacagtctt ttcgtcccag agcagcgcac aatgcctgtt catcccttga tcacccccag
     2641 tggttcgagc ctggccacga ggcgcgagat gccgacttca tgaaccaccc ggaccacttc
     2701 catgcttggt ttatagactt caggtgcctc gtctgcaatc gccgcatcgc tcggcgccct
     2761 gacaataatg ccctcctgcc cgagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnntagtgc atgatcacct ggcggttggc ccatgcgtag ttcgcagagg ctgccatcgc
     2941 cccgaagtat gccttcccct cgggtgagtc gatgggtgca caggccagct gccgatccgg
     3001 aagcgtgatc ccgtatttcc tggtggccgc ctccagggtc cggagatgat cagtgcagac
     3061 ctggtggccc aggccacgtg agccgcaatg gatcatgatg cagacctgcc ctgctgagat
     3121 gccgaaagcc ctggcgactt ccggctggta gacctctttt acgacctgta tctccaggaa
     3181 atggttcccc gagccgagcg ttccgctctg cggcattccc ctctggcggg ctttctggga
     3241 caccgctgcc gtttcagcct ccttcatgca gccctgctgc tcgcacatcc ggacgtcctc
     3301 atcaataccg aaacccgcac cgaccgccca ggacgaccct tccaggagca tgcggtcaag
     3361 ttcatggccg gacagccgga gtgtgctctt tcccccgacg cctgtcggaa ccgctttgaa
     3421 gagtcgttcc accagttccc gctttttcac gaggtcctct tcccggagag gggtggcgag
     3481 cagacggacc ccgcagttga tgtcgaatcc cactccgccc ggcgagatca ccccctcgtc
     3541 aagagagaat gccgcaactc ccccgatggg aaacccgtat ccccagtgga tatcgggcat
     3601 cgcaagggag cgtgccacga tgccgggcat ggtggcgaca ttcgcaagct gctggactgc
     3661 cccctcctcg agggtccctg ccagcgtttc cgaaaggaag aacacccccg gcacgcgcat
     3721 ccccgggacg tatcccaccg ggacctgcca ttcgaattcc ctcactctct gtattcctgg
     3781 cagcatggtc attcacacat caaagatgac atccagtata taagactcct tatcacggac
     3841 aatggagaga cccgagtagg agatcccctt gaccccgcgg ccggcattgt ggatatccct
     3901 tgagaagggc tcgcccctga ccaccccctc gactatcccc tctccaacgc ggacttccgc
     3961 tcccgagatc acgagcccgt ccacctcggt gataaagaga atctcggaga ggaacgcgtg
     4021 catgagcgac tcgaggtcat ctccctgcac aaagacggag cgtgacaggc catccctccc
     4081 agggcggccg tacatcacct gcatcagcgc ccgcccggct tcggagaaga gtccttcgag
     4141 ggtccctgcc ctcacccgga ctcgcgcatc cgcggtatgc tcaagttcct caaaactcat
     4201 ggcccggcga ctggtcctcg atctggtcaa ggaacatcgg cagcattccc ctgtgaatgg
     4261 attggaggta gcgtgcctgg tggatggaaa taaaaatggt gtggtctccc gtgcggacct
     4321 ggaggtctgt ggctttcggg ggtttcatgc tgacaggaag gaggagcggg cccccgcagg
     4381 aggtgcagat ccgaaagtcg catcccctcc tcctgatgta atctgccacg tcttcgttca
     4441 ccacgatatc ttctataggg gtgagtgtat tcggtctcac gtacatgatt ctcaccacat
     4501 cgaccggaaa cggtaaaatg attatgctgg gaactcttca ggtagttccc acgatctttg
     4561 cgacaagatc ggtatattgc tccttgagct ggtagacgct gtgagtccct tcacggttct
     4621 tcatgacgtg gagtatccct atgcgggatg cgatgattcc caccattgca gcaactgagt
     4681 ggtaactgat gttgaacctc ctgttgaggt actcgcagat cttcgcaata gtaagggact
     4741 ttattttgag aaagagttgg agcatggcac gccgtatccc ggtatggtcc cttgaaaggt
     4801 acatccgcag gcggccctca atgaccgctt tgatctcatg cggagatctc atgaattggt
     4861 actcaccata agatttatat aaggattttc tttttttcac cttttaaatg gggcgtcggc
     4921 gcgtaaaaat tcggatgcat ggagagccgc gaatggcggt tgccgacaga tgccggtaca
     4981 aggaaaaatt gcgtgaattc attggcgtcc cgaaggattt tactcttgtc ttgccaaagt
     5041 actgggctat gggactcatc gggtatgatg tgaacaaata ctctccccgg cagatggtga
     5101 tgatccccat ggtcatcctc atcctctcgc tggccatcct cggatacaat atggccacaa
     5161 tcgggatgcc agtgcgcccg gggattgact tctcgggggg taccgcggtc acgctgttta
     5221 cgaccgacac gccgtcgcaa ctggaacaga agttccaggg gtacccgctc ctctctgtgg
     5281 gagaaggggt aaacaacgga aaatacctta agttcgggcc catggacgat acatccgtgc
     5341 agtctcttgc cgcactcatc ggggcggaat accccgatgc caagatcgac cagatcggtg
     5401 cgtctttcgg aaagaccctc cagcagcagg cccttgttgc gttgatcatc tcgttcatcg
     5461 gaatggcgct cgtggtgttt ttggccttcc gtgtgttcgt gccggcgata gcggtgatat
     5521 tctgcgcatt cactgatatc acaatgactg ccgcggggat gagcctggtg ggaatcgacc
     5581 tctcgctccc caccgttgct gccctcctga tgctgattgg gtactcggtg gactcggaca
     5641 ttctcctcac gatgcgggtc ctgaaaaggc agggaaaact tgaagagaag ctctccggcg
     5701 cgttccagac tggaattatc atgaccacca ccactatcgc cgcggtggcc gccatgtggg
     5761 tggtcgcgtt tgcaggccag atcaccgtga tctgggagat cgcaacggtc cttctcattg
     5821 ggctcgtgct tgacatgatg aacacctggc tgacgaatgc agggatcatc aagtggttcg
     5881 tcacaaggcg gggtggaaaa tgagcgacag caagctcatg gcctgcctga agcactggca
     5941 ggtcgccctc atggtcatcc tgcttgtggc gtccgtcatc gccatcgctc ctaggttcga
     6001 ggatggaaaa ttcaccacca acctccagta cggcctggac ctgcaggaag ggacatggct
     6061 gcagatggag ttcaaggctg aagtggtagg gttccagacc gacagatccg tcgaggactt
     6121 cgtgaccgac ctcgagaaac agctcgacgc ggagatctac ctcgtagacg aggggaaact
     6181 ggagatccgc agttattacc ctgaagagac cctcgaaacc atattcgcgt ccgcgggagg
     6241 caagctgacc tcgtatgaac agggcgtcac aagggagacc gcagatgacg tcaagcggat
     6301 ccttgagaac aagctgaacg cgcttggaac caaggacgca cgggtcaacc cccttaccgg
     6361 cctctctggt gtgacccact acatccgcgt ggagcttgca ggagtcagcc tctcacaggc
     6421 caaggagatt gtcggaaagc agggcaagtt cgagatccga atacacacca ctggaaacca
     6481 gacagagcac gtcctgtacg gggacactat cacgagtgtg ggggttccaa gccacgaacc
     6541 gccgggaagt gctgtctggg gtgtctcttt cactctctcc gaggacggag caaagaaatt
     6601 ccagcaggga gcgattgcca cgggtgcagt aagcgaccct gatgcgcact accttgacat
     6661 ggtcctcgac gaaaaggttg tatacagtgc gccgctctca ggtgaccttg cagcgaaact
     6721 cgccattgag cccatccggc agctgtatgc atccacggga accggtcccg cagggctcga
     6781 agcggcaaag aacctcgaga tccacctgag ggcaggggca ctcccggtgg acgtgacagt
     6841 cgccggctct gggtatgtcc ctgcagggct ctcggactat ttcaggatga tgtgcgtgat
     6901 cgctttcatc gctgcggcga ttgcggtcgg ggtggtcatc tattaccggt accgtgaagc
     6961 aagcatcgtg ctgcccatga tcgggaccaa cctcaccgag atcgtgatcc tgctcggggt
     7021 cgcaacgctc atccagcaac tggaccttgc cgcaatcgct gcattgatcg cagtgatggg
     7081 taccgggatc gaccagctgg tcatcatcac cgacgagatc ctgcacgagg ggaaggtacc
     7141 ctcccccaca ctctacaaga agcggctcgc gagggctctt gggatcatcc tggtctccgc
     7201 gttcaccgtc atcatcgcca tgataccact ggcgttgatg gacctctcgt ctctgcgagg
     7261 ctttgccctc atcaccatcg tcggtgtggt aatcggcgtc acggtgaccc gacccgcata
     7321 tggagagatt atcaaggaga tcctctccaa ataaccgata acaaccttta tcaccctttt
     7381 ttctccactt agattcttgg gaattcacat gagcagaccg gtgttactcg acttttatgc
     7441 agattggtgc gggccgtgca gacggcaggg acccatcctc gaggagttga agaagaagat
     7501 gggcgacagg gtggagataa agaagatcga tgtggaccag cacatggaga tggccaacaa
     7561 gtacggcatc cgggtggtcc cgaccctcat catcgagaag gacgggaagg tgatgcacag
     7621 ccttgaaggg gtcacgagtg ccgagacact cgagtccatg ctctctcgcc tggtggatta
     7681 gatgaaggtc ggcatcgtag ggggaaccgg cgacatcggc gaggggatcg ccatgcgcct
     7741 ctcgtcccgc cacgacgtgg tcatcgggtc cagggaggag gagaaggcgg tgcagagcag
     7801 cgagtgctgc ctggaaacgc tcacgaaaaa aggtcttccc tgcagtgtca ggggacacac
     7861 gaaccaggaa gcggtggaca ggtctgacgt ggtcatcctc gcgataccgt tcaggcacgt
     7921 tgagccaacc ctttcatccc tgaaaggatt ctcgggaaag atcgtggtct ccccggtcaa
     7981 ccccatggag aagcgggatt atttcgtgtg tgtcccaccc ccggaaggct cggcggcgtt
     8041 gatgataagg cggctcctcc caccggacgc acgcctctgt accgccttca acacggtcgc
     8101 tgcgaacaag tggcgggcgc ttgacgagga acttgaactt gcgatccctg tctgcggtga
     8161 tgacgcggag gcaaaggcgg tcgtgatgga tctgatcaac agcgttccac gcgtcagggc
     8221 atacaatgcc ggcccccttg ccgcctcctg catggtcgaa tgccttacac ccctgctcct
     8281 caacatcgca cgcttcaaca agatgcgcga tgtcgggatc cagttcaaat aatcccattc
     8341 aaacaaccac aatttttcac gggaatcccc agatgaacca cgcagcagag ttccagagga
     8401 ttggaaaaag actccgtgag gaggggctcg tgagtgcgaa cttcgggaac atgagcgtcc
     8461 ggaaagggga tgggctcctg atcacccgga acggcgcgtt cctggacagt cccggggagc
     8521 tggtgtatgt cgccatggcg ggcgctatcc cacaggaggc atcaagtgaa tgcaagttgc
     8581 acagggaggt ctaccgtatc accccccacc acgcagtggt gcatgctcat ccgcctcacg
     8641 cagtcgccac ctccctgcac cgggaccgca tcgttcctgt cgacagcgag ggcgtgatgt
     8701 tctgcccctc ggtcccggtg gtcaccgggc tgccaggaag catggagatg gcagagaacg
     8761 tggcctccgc tctgaaggat cacaaggtag tcatcgtcag ggggcacggc acagtctgcg
     8821 gcgccggctc cctcgaagag gggtacgtgc tcacctcgat tgtcgagcac agctgccggg
     8881 tgttgctcct ctctcggggc gggtgcgatt gaggccgccg cctcccctcg tcgagaaatc
     8941 aaagaagata ataccttgca atgcagagag ataggcagaa cggggcgtcg ggtaccaatg
     9001 acagggattg aagagaagat ctcaaggctc cctccggaac tgcagaaaga ggtcgaagat
     9061 tacatcgatt ttctcgtcag gcggcacgct cctgcccgtg cacaggagca ggtccctggt
     9121 tttctctctc ctcctcctgc accggcaccc tcaaagccaa tcatccttgc cgacgagata
     9181 cccttccgaa cggaaccaga gatccttccg ggctacaagg atctcggtga gttctctgaa
     9241 cccgaagcac gctccattcc ggtgaaagag ccttccgtga ggacaagacg gccgaaggac
     9301 gccgaacccg gcaggcagct cctggactgg atcgactaaa aactgaattc ggcagcgctg
     9361 aattttttga agggcatctc ccgctccccg acttccgttg cgacctccgg tcccgtattc
     9421 catttcgggg ttaaaataca gtctcttcgc tatctggtgt gggaaggatt ggtgaaccgg
     9481 acacatttta taggggcgga cagcctccac acccccatat gcgatgcaca ccgccgtccc
     9541 cgaagggcgc cccggcggcg gttcaaaaac tattcacatt ggccttgaag aacggagtta
     9601 tcatctgaaa ttcacaatac aacaaaaaaa cccacctcaa tctaatggga taatttcacc
     9661 ttttccttaa aatttcggtg gcaaaattgt cactataccc tagattttaa agaaggaaaa
     9721 aatccaaaaa ccgatggact cgacgggatt cgaacccgtc ttttcgcggg ggcttctgcc
     9781 cctcgacccc gtcgcgacct ccggtcccgt attgcatttc aggtttaaaa tacaggctct
     9841 ttgctatctg gtgtgggtag gattggtgaa ccggacacat ttcgtgggag ctgtccgccc
     9901 ccacaccccc atatgcgatg cacaccgccg cccccgaagg gcgccccggc ggcggttcaa
     9961 aaactattca cattggcctt gaagaacgga gttatcatct gaaattcaca atacaacaaa
    10021 aaaacccacc tcaatctaat gggataattt caccttttcc ttaaaatttc ggtggcaaaa
    10081 ttgtcactat accctagatt ttaaagaagg aaaaaatcca aaaaccgatg gactcgacgg
    10141 gattcgaacc cgtggccttt cctcttgcag accgaaggct tcgcctttcg aaaccatggc
    10201 gacctccggt cacctccttt tcattccaag taagaaatcc gatatattta aaaaagaagg
    10261 aaaaaatcca aaaaccgatg gactcgacgg gattcgaacc c
//
LOCUS       DAOQYR010000079        10245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_7003_length_10245_cov_8.498626, whole genome shotgun sequence.
ACCESSION   DAOQYR010000079 DAOQYR010000000
VERSION     DAOQYR010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 10245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10245
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7003_length_10245_cov_8.498626"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..1501
                     /locus_tag="PKM87_09050"
     CDS             143..1501
                     /locus_tag="PKM87_09050"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HNQ30251.1"
                     /translation="MAGYTLSFGRDPHYVELMERALDHAPNGGFCNKTDPSGVPYLAV
                     VNQQRALVGITDSSATGITISPYQWYWNTIQSSRNTTPGHYPYDLGTWWNNVKDLIDD
                     STSPSEVLNWFWALDTYGTPDYSYFGYSSDAASKKLSQPLCAAFTSSQDQTTFVAWNP
                     HGEPVHVNWWKVGEKAGSAAGVLAQDLIVPPGWYATAGTTPPANASLVVTIDAPVSTR
                     PGSTVTFNVTVRATGPRQVSGVAVSSYLPYSMTYVSSTPAGTVSGNSVRWDAGTLSPG
                     ETRSYLVTGKFPDTANGQYTAVAEASGKAAGNIAVKGNRILPISVQGGPSMNVSISAD
                     KAAVRRGENITFTFVLRNDGTQDLEGAYAKLNFQPVGGLSFISANYAPQYTAPDTAIW
                     DPPHLPGTGKLSIGTNITITTTYTVTSSARDPLTTTAYGQANYAGGQANNTVTLTTGV
                     TG"
     gene            1498..3354
                     /locus_tag="PKM87_09055"
     CDS             1498..3354
                     /locus_tag="PKM87_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ30252.1"
                     /translation="MKKNSMCAGIAALLIVLCLAAVPALAASTGIAWERSLGGSGEEY
                     AKSLQPTNDGGYIAVGDTASTDGDITVSYGMIDVWIAKLDASGEKEWEKTIGGSNDDH
                     VTCIELMSDGTLLLSGYTWSHDGDFSDNHAGIAIWNMQINEEGSILWKGCYPGRSGSF
                     AMDGKETPDGGMIMIGQSFSIPGDPMYHGGYDVLVVKTDQSANTTWERLLGGSGDDFG
                     DNILPLPGGGYIAIGYTNSDDGDVSGNHGFYDAWLFRLDDAGNLLWQKCLGGSLHDEG
                     REIFITPDGNFAVMGYTNSNDGDVSGNHGSFDYWWALVASDGAILSQKCFGGSDYDHA
                     YDLEMTDNGNFVALGVSYSTDGDVSGNAGNGDFWAVEFDTTGAILWERCFGGSGLDRG
                     QTIRPDPSAGGLFFLIGDSASTDKDVTGNHGGSDYWIVKVGGTEGLSADFKAEPVTGT
                     APLAVRFTDRSRGDPTWYIYEFGDGFRGMNQNPVHTYRKAGTYNVTLTVVKVNPETKR
                     VEKDSVTKTGCIVVTKGSSVPFVSDFSATPTTGTAPLTVRFTDQSTGSPGYFLYEFGD
                     GFKAMGANPVHTYRKPGTYTVTLTIMKVDKDMGKILRNTTSRTQMIVVAGQG"
     gene            complement(3738..4157)
                     /locus_tag="PKM87_09060"
     CDS             complement(3738..4157)
                     /locus_tag="PKM87_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30253.1"
                     /translation="MRRDVLFLIIAVICVAALGMSLSLLLPGQARAGITSVATDKDLY
                     HSKETMMITVSVDSQGNIGNATLRLLGIQDRYGDYQLRKDIPVNLSHGANTLVYDHKL
                     PSCSSCSGLAAGTYRIDVSLLHDGVLLSNTTHSFQLA"
     gene            complement(4373..7030)
                     /locus_tag="PKM87_09065"
     CDS             complement(4373..7030)
                     /locus_tag="PKM87_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ30254.1"
                     /translation="MSTTGRERGKKPGMDFSFSHYLLFSMILLILVMAMGITYFDYRH
                     AEETYQKSARAMQEQTENDIAKTIRIVDSAYAMYDQTLNLQMMNGFSLFLGEYERAGR
                     DPSRMDLLSLKDEMGNIMDLYIINESMMVEATTYEPDLGLDFSRWPHSYNFLKDLIQK
                     DGFFPDRVVYEVSSGKLRKYAYMPTPDHRYILELGMTEKGIGERPAIHYQEPLREMAF
                     NNPHILSYRAFDTVGRLLGKGSDSQLKSPGILKQVVEERRTIEVEDSYNNTVIRYLLV
                     DLSEQSYASDTSWIIELVYDQSGIKTQLADLVFYHLLVALIAVILSAGIAIVLSRQLM
                     KPVKRMVDDIDTISKGDLDHTIAHANIREFSRIEESITALVTTLKGMIGRLRQSEAEL
                     KMSEENYRTVVENQTELIARFLPDGTHLFVNDAYCRYFDIPCSELLGKKMWPRIPEED
                     TLLLARHFASLTPENPNSTIEHRILTPGGEVRWLQWNDRAIFGPDGTVTEYQSVGRDV
                     TEKKRIEQDLVESEKKFRDLASLLPQVIFEIDTTGRITYVNQPAFHRFGYSPEELEGG
                     INFTQVIKPEERMEAIQNFIGIMEGRKEAGAEYHMVRRDGSELTGMVYSSPILKDGKA
                     AGIRGILVDITQLKQVEEDLRRLNEELESRVADRTRDLEDANRELEAFSYSVSHDLRA
                     PLRAIDGFSSILLTEHAASLDPAVKGLLLRIRSNAQKMGQLIDSILNFSRMSRQPLSK
                     QTVYPGQIVQDVLEELKTMQEGRNIEVRVNSLPSCEGDPALIKQVFFNLVSNAFKFTR
                     TRERAVIEIGSFEKDGKAVYMVRDNGIGFDMKYADKLFSVFQRLHDEKMYEGTGIGLA
                     IVYRIIQRHGGRIWVESEVDRGTTFYFSF"
     gene            complement(7501..8619)
                     /locus_tag="PKM87_09070"
     CDS             complement(7501..8619)
                     /locus_tag="PKM87_09070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ30255.1"
                     /translation="MSPPPPLPSGNFFDIIPLGVVILAPDYTVVSWNRTIAEWTGIPR
                     NEILGNDIRERFSHLKDRRYNIRIQQVFDGGPAAFFSTQFHPHFIPAPLPGGGLRFQR
                     TSVHSLQEGGETYAILFIDDVTELVAQVRAFREMKDRALAEVDERTRKEAALALANKK
                     IGLLSSITRHDILNQVMALKFYIETMREETNPQQVAALLAKEMVVANAIERQIHFARD
                     YQEMGIHAPGWQRVASIVERSKEALPTGNISVVANVGSIEVYADPLLEKVFYNLIDNA
                     LQYGGPTMSTIRISTNENGDGLVLTIEDDGSGISEMDKPHLFTRGYGKHTGFGLFLSR
                     EILSITDITIQETGEPGGGARFEIRVPMGKFRHSGTVP"
     gene            complement(8616..9224)
                     /locus_tag="PKM87_09075"
     CDS             complement(8616..9224)
                     /locus_tag="PKM87_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HNQ30256.1"
                     /translation="MNIDEDSVDAIRELINIGVGRAAGLLNEMTGTHIRLMVPEVRIL
                     RYADLVSENRILEQDRLSAVTLKFEGSFSGISMLVFPPDSAAILITALTGEEIRAPDL
                     DALRIETLNEVGNIVNNAVMGSITNVLNERLTYSMPTYLEGSIAQILGNRRAIDYDWV
                     ILAISQFSLADLQVVGTILMIFEIGSLDSLVKRLQDAAGQER"
     gene            complement(9221..9592)
                     /locus_tag="PKM87_09080"
     CDS             complement(9221..9592)
                     /locus_tag="PKM87_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ30257.1"
                     /translation="MPKVLIIDDSGFQRKIITSILEAEGNMVLTAENGRTGFDIAVKE
                     SPDLIICDLLMPELDGYGLLRLVRDHKLAIPVLILTSDIQNTTRTECLALGALDVLNK
                     PVKKETLVPIIKDALRSAKRP"
     gene            complement(9728..>10245)
                     /locus_tag="PKM87_09085"
     CDS             complement(9728..>10245)
                     /locus_tag="PKM87_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30258.1"
                     /translation="FAGCTQPSPVTQTTPAPTATTAAPTLTPTASAANETLKTELASL
                     AAGFAKEIDGQALAAVLKEGPNSTAFAGVLGQLKAFKERDPRIAYVYTLEQQNGTVMF
                     IVDAHYGHPDASQYLEVYDDAPIELKTPVTSPIGVGPYTDKWGTFYSGYAPVDAGSNA
                     TVILIGVDVVA"
BASE COUNT         2241 a         2831 c         2889 g         2284 t
ORIGIN      
        1 agtcacgtcc tcctggccgg tgatgccgga cgggtacccg aagaccacct cgtttgcccc
       61 gaagatgccc cagtcagtgg tccagaccgc ctcgcagttt gggatctccc ccacctcgtc
      121 catgtacaac atgtggctcc cgatggcagg atacacgctc tcgttcggga gggatccgca
      181 ttacgtggag ctcatggaac gtgcgcttga ccatgccccg aacggcgggt tctgcaacaa
      241 gaccgatcca tcaggtgtgc catacctcgc ggtggtcaac cagcagcgtg cccttgtagg
      301 aatcacggac agttcggcga cgggaatcac catctctcca taccagtggt actggaacac
      361 catccagtcc tcgcggaaca ccaccccggg ccactacccc tacgacctcg ggacatggtg
      421 gaataacgta aaagacctga tcgatgattc cacgagccca tctgaagtcc tgaactggtt
      481 ctgggctctc gacacgtacg gtacccctga ttactcgtat ttcggatact cttccgacgc
      541 cgcctcgaag aaactctcac agccgctctg tgccgccttt acttcaagtc aggaccagac
      601 aacttttgtc gcgtggaacc cgcatggcga acccgtgcac gtgaactggt ggaaggtagg
      661 ggagaaggct ggttccgctg ctggtgttct tgctcaggat ctcattgtgc caccgggatg
      721 gtatgcgacg gcaggcacca cccctcccgc gaacgcatcg ctcgttgtga ctatcgatgc
      781 tcctgtaagt acccgccccg ggagcacggt caccttcaac gtgaccgtca gggcgaccgg
      841 cccaaggcag gtttccggtg tcgcggtctc cagctatctc ccgtactcta tgacatatgt
      901 ctcgagcact cctgccggga cggtctccgg caattccgtc agatgggatg caggcacgct
      961 ctctcccggg gagaccaggt catacctcgt gaccggtaag ttcccggaca ccgccaacgg
     1021 gcaatatacc gcggtcgccg aagcatccgg gaaggctgcc ggaaatatcg cggtgaaagg
     1081 caaccggatc ctccctatct cagtccaggg cggtccctcg atgaacgtct ccatctctgc
     1141 agataaggct gcggtcaggc gtggggagaa tatcacgttc accttcgtcc tcaggaacga
     1201 tggaacacag gaccttgagg gagcgtatgc gaaactgaat ttccagccgg tcggtggtct
     1261 ctctttcatc tccgcgaatt acgcacccca gtacactgcc ccggatactg ccatatggga
     1321 cccgccacat ctcccgggca ccggcaaact gtcaatcggg accaacatca cgatcacgac
     1381 gacctatacg gtcacatcca gtgccaggga tcctttaacc acgaccgcct acgggcaggc
     1441 gaattatgcc ggcggacaag cgaacaatac cgtgactctc accacggggg tgacgggatg
     1501 aagaagaatt ccatgtgtgc agggattgcc gcacttctca tcgtgctctg cctggctgca
     1561 gtcccagcgc ttgcagccag taccgggata gcatgggaga gatctctcgg cggatccggg
     1621 gaagagtatg caaaatctct tcagcccacg aacgacgggg gctacattgc cgtgggggac
     1681 accgcctcga cagacgggga catcacggtc tcatacggga tgatcgatgt ctggattgca
     1741 aaactcgatg cgtccgggga gaaggaatgg gaaaagacca tcgggggatc gaatgacgat
     1801 cacgtgacct gtatcgagct gatgagtgac ggcaccctgt tgctctcggg ctatacctgg
     1861 tcgcatgacg gtgatttctc tgacaaccac gccggaatcg cgatatggaa catgcagatc
     1921 aacgaggagg gatccattct ctggaaaggg tgttaccctg gtcgaagcgg cagttttgcc
     1981 atggatggca aagaaacccc tgacggaggg atgatcatga tcggccagtc attctctatc
     2041 cccggagacc cgatgtacca tggcggctac gatgtcctgg tagtgaagac ggaccaatct
     2101 gcaaacacca cctgggagag gttactcggc gggtcggggg atgacttcgg ggacaacatc
     2161 ctcccgctcc cgggcggagg gtatatcgcc atcgggtaca cgaactcgga cgacggcgat
     2221 gtctccggga accacggctt ctacgacgcc tggttgttca ggcttgatga tgccggaaac
     2281 ctgctctggc agaagtgcct cggggggagc ctccacgatg aaggccggga gatctttatc
     2341 actcctgacg gaaattttgc ggtgatggga tataccaact cgaacgacgg ggatgtttcc
     2401 gggaaccacg gttcattcga ttactggtgg gcgctggttg cctctgacgg agcgatcctt
     2461 tcacagaaat gtttcggagg gtcggactat gatcacgcct atgacctcga aatgaccgat
     2521 aatggaaact ttgtggcact cggcgtctct tattcgaccg atggagatgt cagtggaaat
     2581 gccgggaacg gtgacttctg ggcagtcgag ttcgatacga ccggggcgat cctctgggag
     2641 cggtgcttcg gagggtcagg acttgaccgg ggccagacca tcaggcccga tccttctgca
     2701 ggaggcctct tcttcctgat cggtgactcc gcatccactg acaaggacgt caccggcaac
     2761 cacggcgggt cggactactg gattgtgaag gtaggaggaa cggaaggact ttcggcagac
     2821 ttcaaggctg aacccgtgac cggcacggcc cccctggcgg tccggttcac cgatcggtcg
     2881 aggggagatc ccacatggta catctacgag ttcggagacg ggttcagggg aatgaaccag
     2941 aatccggttc atacctaccg caaagcaggt acctacaacg tgaccctgac ggtcgtgaaa
     3001 gtcaacccgg agacgaagag agttgagaaa gattccgtca cgaagaccgg gtgcatcgtt
     3061 gtcacgaaag gcagcagtgt tccattcgtt tccgacttct ccgctacccc aacgaccggc
     3121 accgccccgc tcactgtccg gttcaccgac cagtccaccg gaagcccggg atacttcctc
     3181 tacgagttcg gggacgggtt caaagcgatg ggcgcaaacc cggtgcacac ctaccgcaag
     3241 cccggcacgt acacagtcac cctgaccatc atgaaagtcg ataaggatat ggggaagata
     3301 ctgaggaata ccaccagccg gacgcagatg atagtcgttg ccgggcaggg atgagcccgg
     3361 tccaatccgg ttcttttttt ataagtggga atgagtgctc tttgactggg tgttcggccc
     3421 gaaccacacc tgattttttc gcctcctcgc ggttttgtgt gggggactga attgaggtta
     3481 ttgtatgcat acaaattcag gcactccagc cgccgcgggg gcgccccgtc gggggcggac
     3541 agaccccaca aaatgcatgc ggatcgtcaa tcctgcccat accagagagc aaagagccat
     3601 ttgtttaaaa tgaataattt ctgcaccact tcaccggcga attttagtcc agttgagccg
     3661 gaaatattgc aaacccggtg aaagaggggt ttcacgctcc agggtgattg agtgaacggt
     3721 ccctttacag gcatgggtca cgccagctgg aacgagtggg tagtgtttga gaggagaacc
     3781 ccatcatgga gcaggcttac gtcgatccgg tacgtccccg cagcaagccc cgagcaggag
     3841 gaacaactgg gcagcttgtg gtcatagacc agggtatttg caccgtggga gagattgacc
     3901 ggaatatcct tgcgaagctg gtagtcgccg taccggtcct ggatgccaag gagacgcagg
     3961 gtggcgtttc caatattccc ctgcgaatca acggacactg tgatcatcat ggtctctttt
     4021 gagtggtaga gatccttgtc tgtggcaacc gatgtgatgc ctgcacgcgc ctgaccgggg
     4081 aggagcaaag agaggctcat tccaagggcc gcaacacaga tcacagcgat gatgagaaag
     4141 aggacatccc gtcgcataaa aagaggtggc actgtgtggc tatatataca tcaggtcccg
     4201 agagtccaag ggaggagtcg tctccccata tcctggcact catccactca cgcaagggat
     4261 cggaacgcgg gaggaggcat gagggcgcaa gggacacggt ctcaagctgc aattctctct
     4321 ctccgaaatc cggatgtgtt acactggaga tgccagagtg aatattctct cttcagaaag
     4381 agaaatagaa cgtggtcccc ctgtcaacct cgctctcgac ccatatcctc cccccgtgac
     4441 gctggataat acggtacacg attgcaagcc cgatcccggt gccttcgtac atcttctcat
     4501 cgtggagccg ctggaagaca gagaagagct tgtcggcata tttcatgtca aagcctatac
     4561 cgttgtccct gaccatgtac accgcttttc catccttctc aaaagatcct atctcaataa
     4621 ctgcacgctc ccgtgtcctg gtgaacttga acgcgttgga tacgaggttg aaaaagacct
     4681 gttttatgag cgccgggtcc ccttcgcatg aggggagcga attgacacgg acctcaatat
     4741 tccgcccttc ttgcattgtt tttaattcct ccagcacatc ctgcacgatc tggccgggat
     4801 acaccgtctg cttcgagaga ggctgccggg acatccgcga gaagttcagg atggagtcga
     4861 tcagctgccc catcttctgt gcattggacc ggatcctcaa gagaagccct ttcaccgcgg
     4921 gatcaaggga ggctgcgtgc tcggtcagga gaatggaaga gaacccgtcg attgcgcgca
     4981 gaggcgcacg gaggtcatgg gacaccgagt atgagaatgc ctccagttcc cggtttgcat
     5041 cctcgagatc tctggtacga tctgccacgc gggactccag ttcttcgttc agccggcgga
     5101 gatcctcctc aacctgcttg agctgcgtga tatcgacgag gattccccgg atgccagccg
     5161 ctttgccatc cttgagtatc ggtgaggagt agaccatgcc agtgagttca gatccgtccc
     5221 tgcggaccat gtgatactca gcgcccgcct ctttgcggcc ttccattatt ccgatgaagt
     5281 tctgaattgc ctccatgcgt tcttccggtt tgattacctg tgtgaaattg atcccgcctt
     5341 cgagttcttc gggactatac ccaaagcgat ggaatgcggg ctggtttaca taggttatcc
     5401 ttcctgtggt atcaatctcg aagatgacct ggggaagaag actggcaaga tcgcggaact
     5461 tcttctcgct ctccacaagg tcctgctcga tccgcttctt ctctgtcacg tcccttccca
     5521 ccgactggta ttcggtaacc gttccgtcgg gcccgaatat tgcccggtca ttccactgga
     5581 gccagcgcac ttcgccgccg ggggtgagaa tgcggtgttc gatggtagag ttcgggttct
     5641 ctggtgtgag cgatgcaaag tggcgtgcga gaaggagggt gtcctcttcc gggattcggg
     5701 gccacatctt cttccccagc aattcgctgc acgggatgtc gaagtacctg caataggcgt
     5761 cattgacgaa caggtgcgtc ccgtctggca ggaatcgtgc gatcagttcg gtctggttct
     5821 cgacgacggt cctgtaattc tcttcgctca tcttcaactc ggcctcactt tgccggagtc
     5881 tcccgatcat gcccttcagg gtagtgacaa gggcggtgat actctcttcg atgcgtgaaa
     5941 actcgcggat attcgcatgg gcgatcgtat ggtccaggtc ccctttcgag attgtatcga
     6001 tatcatcgac cattctcttc acgggtttca tgagctgccg ggaaaggaca atggcaatcc
     6061 ctgcgctcag gatcaccgcg atgagcgcta cgaggaggtg gtagaagaca aggtcggcta
     6121 actgggtctt tatccctgac tggtcataga caagctcgat gatccagctg gtatcagatg
     6181 cataactctg ttcagagaga tcgaccagga gatacctgat aaccgtgttg ttgtaagagt
     6241 cctccacttc gattgttctc ctctcctcga cgacctgctt caatattccc gggcttttca
     6301 actgggaatc gctccctttc ccaagcaacc gcccgacagt atcaaatgcc ctgtaactga
     6361 gaatgtgtgg attattgaat gccatctcgc gcaacggctc ctggtagtga atggccgggc
     6421 gttccccgat gcccttctcc gtcatgccaa gctcgaggat atagcgatgg tcgggtgtcg
     6481 gcatataggc gtatttccga agttttcccg atgagacctc gtacacgaca cggtcaggga
     6541 aaaatccgtc cttctggatg agatccttta aaaaattgta agaatgcggc catcggctga
     6601 aatcaaggcc gagatcaggt tcatacgtag tcgcctccac catcatcgac tcgttgatga
     6661 tgtagaggtc catgatgttc cccatttcat ccttcaggga aagcaggtcc atccgggacg
     6721 gatctcttcc cgcgcgctcg tattccccaa gaaagagaga aaacccgttc atcatctgga
     6781 ggttgagggt ctggtcatac atggcgtacg cagagtcgac gatcctgatc gtcttggcaa
     6841 tgtcattctc tgtctgttcc tgcatggcgc gggcactttt ctggtaggtc tcctctgcat
     6901 gcctgtagtc aaagtaagtg attcccatgg ccattacgag gatgagaagg atcatggaga
     6961 agaggagata atgggagaat gaaaagtcca taccaggttt ctttccgcgt tcccgccctg
     7021 ttgtgctcat gttacgctcc gatcaaccag ctctttttca tttccgcaac cgagggtaag
     7081 ggtgtggaca tttacgatga accgaacatg tcgttccagt catggaataa attccacggc
     7141 ccatggatgg gatgtggggt gagagcgata ccccccggtt acctctcacc agagcatctt
     7201 tttgatgtcc ttaactcatc cacagactgg ataaagctgt tgcccgggcg gaagaaatat
     7261 tcaaatcctt tgcggtatac aaaaagaaaa ccaaaattgg ggatcaattt atttaaacac
     7321 gtggccgttg gaagaaaacc tggcgaattc ctaaaaaagg cgagggcgac ccggataggg
     7381 catatcacct ggcagggtgt tctccatgac gcggtggaga ttttcaaaca atccatccaa
     7441 acgaatcaca tcactcttca aaggcggggc cacacgccca cctctccagg gggggaggcg
     7501 tcatggaaca gtcccggaat gccggaactt ccccatcggg acacggatct cgaaccttgc
     7561 ccctccccct ggctcccctg tctcctggat agtgatatcg gtgatagaga ggatctcgcg
     7621 gctgagaaaa agcccaaaac ccgtgtgctt cccatagccg cgtgtgaaga gatgcggctt
     7681 gtccatttcg gagattcctg agccgtcatc ctcgattgtg aggacgaggc cgtccccgtt
     7741 ctcgtttgtc gagatcctga tggtgctcat ggtggggccg ccgtactgga gagcgttatc
     7801 gatgaggttg tagaacactt tctccaagag cgggtcggcg tatacctcga tggagccgac
     7861 attggcaacg acgctgatgt tgcctgtggg gagggcctct tttgaccgct cgacaatcga
     7921 ggctacgcgc tgccagcccg gggcatggat gcccatctcc tggtagtcgc gtgcgaagtg
     7981 gatctggcgc tcgatggcat ttgcaaccac catctccttt gcgaggagag ccgcaacctg
     8041 ctggggattg gtctcttcac gcatagtctc gatataaaac ttcagggcca tgacctgatt
     8101 caggatgtcg tggcgagtaa tactcgagag caggccgatc ttcttgttgg caagtgcgag
     8161 tgccgcctct ttcctcgtgc gctcatcaac ctccgcgagg gcacggtctt tcatctcccg
     8221 gaatgcacgg acctgtgcga caagctcggt cacgtcgtcg atgaagagga ttgcgtaggt
     8281 ctctcctcct tcctgcaggg agtggaccga cgtccgctgg aagcggagcc ctccaccggg
     8341 gaggggtgcg gggataaaat gggggtggaa ctgcgtcgag aagaatgccg caggcccccc
     8401 gtcgaatacc tgctggatgc ggatattgta gcgccggtct tttaggtggg aaaaccgttc
     8461 ccgtatgtcg tttccgagta tctcattcct gggaattccc gtccattccg caatggtcct
     8521 gttccatgaa acgacagtgt agtccggtgc caggatcacc actcccaggg ggatgatatc
     8581 aaaaaaattt ccagagggga gtgggggagg gggactcatc tctcctggcc tgccgcgtcc
     8641 tggagcctct ttaccaggga atcaagtgac ccgatctcga atatcatcag aatcgttccc
     8701 acgacctgca ggtccgcgag agaaaactgc gatatcgcaa ggataaccca gtcgtagtcg
     8761 atggctctgc ggttcccaag gatctgggca atgctgccct cgaggtatgt gggcatgctg
     8821 taggtgagcc gttcgttcag gacgtttgtg atagacccca tgaccgcatt gttcacgatg
     8881 tttccgactt cgttcagtgt ctcgatcctg agcgcgtcga ggtccggcgc cctgatctcc
     8941 tcccctgtga gcgcggtaat caggattgca gcactgtccg gcggaaacac cagcatgctg
     9001 atccccgaga acgacccctc gaatttaagg gtgactgcag aaagtcggtc ctgctccagg
     9061 atccggttct cactgacgag atctgcatac cggagtattc tcacctccgg gaccatcagc
     9121 cggatgtggg tcccggtcat ctcattgagg agccctgcag ccctgccgac cccaatgttg
     9181 atcagttcac ggatggcatc aaccgaatcc tcgtcgatgt tcatggccgt ttcgcgctcc
     9241 tgagcgcgtc ctttattatg ggaacaaggg tctccttctt taccggcttg ttcaggacat
     9301 cgagggcacc aagggcaagg cactcagttc ttgtcgtatt ctggatatcc gaggtcagga
     9361 ttagcaccgg aatggccagc ttgtgatctc ttaccagcct cagcagtccg tacccgtcaa
     9421 gttcgggcat caggaggtcg cagatgatga gatccggaga ctccttaacc gcaatgtcaa
     9481 aaccagttct tccattctca gcggtgagga ccatattgcc ttctgcttcc aggatcgagg
     9541 tgatgatttt tcgctgaaag cccgagtcgt caatgatcag gacctttggc atcgcgatat
     9601 ctcctttgct atgggattgt atcattcaat cctgtttatc tctttatcga ctgcacagcc
     9661 cactgaagga tggcgacccc gtgcagggcc cagggcgaaa tgaatgaaaa aaggagggag
     9721 tgctggatca ggctaccaca tcgaccccga tgagaatcac ggtcgcgttc gaaccggcat
     9781 caacgggtgc atagcccgaa tagaatgttc cccacttgtc cgtatagggc cctaccccaa
     9841 tcggagaggt gacaggggtc ttcagttcga tgggggcatc atcatatacc tcgaggtact
     9901 gggaagcgtc ggggtgtcca taatgtgcat cgacgataaa catgacagtc ccattctgct
     9961 gctcgagggt gtagacatac gcgatccggg gatcgcgttc cttgaaggct ttcagctgcc
    10021 cgagcacccc ggcaaacgcc gtcgagttcg gcccttcctt aagaactgca gcgagtgcct
    10081 gcccgtcgat ttcttttgca aaacctgccg caagtgaagc aagttcggtc ttcagggtct
    10141 cgttggctgc ggaagcagtc ggggtgagcg tgggtgcagc agtcgtggca gtaggcgcag
    10201 gagtggtctg ggtaacggga gaaggctggg tacatccagc gaaga
//
LOCUS       DAOQYR010000080         9939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_7285_length_9939_cov_9.153683, whole genome shotgun sequence.
ACCESSION   DAOQYR010000080 DAOQYR010000000
VERSION     DAOQYR010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 9939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9939
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7285_length_9939_cov_9.153683"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKM87_09090"
     CDS             <1..252
                     /locus_tag="PKM87_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30259.1"
                     /translation="TYRSPDTVPVCVVESGRVAADTAPGIARSNKRIKRLMLVICFII
                     QYSNRGWHSADIQKIRSGYHKYIMIFFYILHTKFNISKT"
     gene            complement(448..3702)
                     /locus_tag="PKM87_09095"
     CDS             complement(448..3702)
                     /locus_tag="PKM87_09095"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3344 domain-containing protein"
                     /protein_id="HNQ30260.1"
                     /translation="MNKMDGNRKKGWNLPGSLALISGVLILLLMLQAQGSADPYVGGM
                     PFETVQSGMVTGDLFIDAMVPSWGQRDIAKKFSLPVFTQVRWARLYVAVYCGHMQNNY
                     NGTAKISFDGDGDGTYETVLGTENLDVPFTYVYDGGPGYVMVNSHVNRVTSDYLMWYD
                     VGSKITSRNPGARVETIQQNPSLDGRIKAVTLVVAYDDGDSDQVWYAVNQGHDSDTYY
                     SDEQLGEDYRGETSFDPSGIFDSVEKATLSVNHLASQDGYYRFNGEDLEGGQNQGAYF
                     GFNQWDVTGEVKSDGDNLLTYDRYGGSGSGSEGQFYKIILATLTIRQGTEPEEPPDEE
                     EPTPTPAGSTVSDSSDTGEVATAENGYYGQTIPVLLTGMVNGSVNLYETSDYSDLILS
                     GESKNYTLQVPLRTGDELRLARLYVYTTWDHDEKKREGLAAPLTLQYESTPISPAAHY
                     SDRKGFGIYDYPATTFSYDIQDLVKETGNYTFSVGNAGKGDEAFAVYGLLLILATETE
                     GGTPTLYWIAEGSDVLLAGPEYETTTEDATTVFPFEEVPAFASTAHLFLISTAASGEP
                     DDENRITFNGAEWDNLLVNGSSATSIAMVDVLESIRPGSNEATIQSYLTLKRGDYLEN
                     RGAVLVVVPGDAGLPAAPTTTRTAPVQVTGTAVPAETEVIVTLTPVQQDTGFFGWMFQ
                     RIFAPIMPSSAQSLGPIAPVVALSEEVNLTVITDPEGALVTVNGVEYEDLTPLTCPVP
                     RGGTQEVTVTLDGYDPYRASLKTAGDYDLRIIFTPYVPKKPVVSTSSPSGNGKLGGVY
                     VESYPSEATILIDGRRTDQVTPRVVGGLREGYHTVGMDKKNTIFPAARRIYVARGSIT
                     MISFMEGAMFARMLNITSEDYAGSAFTVNGRGPPVEMPGEAEAEGTPAFIVILHDGSY
                     ISRNVLPQLESGDTMVIRSEPADSQSLTVVISSDPPGSDILFDGFSTGLRTPAAVTNV
                     SPGLHRVTVQAPGYLPGEKELSMIDAMNNPIDEKVFIKLEPYSHGSLNLTSTPAGAKV
                     YLSGRNTGEKTPVIIPSLATGIYTVKLVGEKESRTFEVVITPGQEKAVHAVFPA"
     gene            complement(3734..4945)
                     /locus_tag="PKM87_09100"
     CDS             complement(3734..4945)
                     /locus_tag="PKM87_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ30261.1"
                     /translation="MISDVFSIAFRHLWRKRLRFFLTVLGITIGVAAVIATVSLGEGI
                     RYNAVETIKTQSDLTLITVQPKVHGDVVQLITGARIMEIRNLSSVTGAEGITRDTYAT
                     ARQTFIPVTGIDSVEEGGLLNLRYSRGRPFSPETREVVAGYRLGEKLQRYEGIRQNDR
                     FVVTIREYDASGKPLDSEIELTLVGVLQERGDSIDEILLANRGAVVAARTDAPLYDGI
                     LVRVDSPEHVAGVAAAITGMQLGATGAFEQIESVNRLMDMVVLLLALFAVISLVVGAL
                     MIMNTMMTSVYERVREIGITMAVGASRRDVIRLILAECLIIGIAGGVLGCFCGMVFAA
                     LVNTAGRSFILSRLGEEFTLLFGTEIARVTPGLLIAGLVVSVLLAVLSGLYPAVKAAR
                     LNPVEAIRSGG"
     gene            complement(4942..5619)
                     /locus_tag="PKM87_09105"
     CDS             complement(4942..5619)
                     /locus_tag="PKM87_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ30262.1"
                     /translation="MTSDTRSIIEVESVSKRYTETVSALRDISFSVAPGEFLTIVGRS
                     GSGKSTLLNIIGGLDHPTSGNVRISGALTDYHNHHALVRLRREMVGFVFQQFNLLPAM
                     TAADNVAYPLLFHGRPRQERQERALELLSLVGLADRTGHYPAELSGGEQQRVAIARAL
                     AANPPIVLADEPTGNLDSKTSREIYRLLRELNTDQGITFLVVTHERDLAGYSDRSIEL
                     LDGQVVA"
     gene            complement(5609..6595)
                     /locus_tag="PKM87_09110"
     CDS             complement(5609..6595)
                     /locus_tag="PKM87_09110"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3344 domain-containing protein"
                     /protein_id="HNQ30263.1"
                     /translation="MSSPDLPTCSIICLAVVTLICPGAATYAGDHPLNLAYTGTVPGD
                     YLFSLANGTYSGSINAGSGYDTVLSIGIPEGSDLLYQRVYIYWSWSKKGQAAIYPRMD
                     VQLVPPGEGGALHFRNRYTDNKGFASQNDFYSGMDTYEIPSAMDFSRPFTIRIVNTGE
                     EGTSFTIQGVGFLAVYTVPGGQQKQIQIMEGTDLLYSRYGITPEMATSRIEYPGTISL
                     EQVHDAEMLLVAPSGGYSLSGVPEMNRLFFNRGDEAVVPIVLRPVMHVLFPSFSGKTW
                     TDIFSSSELQQVGVDRREVKPFLRPGGNRAEVQDNGDYIQLTNAVLEVTYDE"
     gene            complement(6582..8375)
                     /locus_tag="PKM87_09115"
     CDS             complement(6582..8375)
                     /locus_tag="PKM87_09115"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3344 domain-containing protein"
                     /protein_id="HNQ30264.1"
                     /translation="MRYALPITIILIVIMLLLSVPCSAMYSFEGVPLGITAQGAIRGE
                     VVSSWSLGLSAPPYSHEFALEHQPAWARVYTGVWGGTEKYRGWTQIDINGKKQEKIEL
                     FGEDDMDRQVYESGNGVYWISFDGTGVIGQGDNTITVTTSRGEPNNKLDGRVYGIIVV
                     ALIESENGPVTQYWIADGNENLHGEGWAGANPTRHDSSAVTFSAPSSGAIRSAELTTL
                     LLTSTRGQPDYITFNGQDLGILAIPESEYLPGARDIGNERSSDANGKSGIDTRYADLE
                     VFDVTSLLKESNTVVFERGRDLNGDGVIVTASALSEGEDYIHPVLAMLVVTENGDSLV
                     PELSVDPITIQGAYQGETATISAVIRNNGIVSSAPVEVVFSVDGVPVATTQVIIGYEG
                     IREVEGSWQAAPGDHTVTVTVKGDTDTSNNAASRSITVGSLPDLSVSLGDPGSPGTGG
                     TSPKNAPFPFAGAIAGGLVLAWWMARKRPSPVTLPALFLTIGIFALVLVPSVAGAGSG
                     VSEFPIPVTVRNTGGSNAPAFVLSLYLDGEKVAEQTVTEGVSAGGAITVTIPLFTTAG
                     RHTLRVVADERGTIPEKDRANNSDERVYEFS"
     gene            8707..8910
                     /locus_tag="PKM87_09120"
     CDS             8707..8910
                     /locus_tag="PKM87_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30265.1"
                     /translation="MITMECPSTAKLSGTQLDEIRKLEGKLGVILVAYDKVHPYKKLT
                     NEALSKIQSVEKDTGSILIAYEA"
     gene            complement(9036..9404)
                     /locus_tag="PKM87_09125"
     CDS             complement(9036..9404)
                     /locus_tag="PKM87_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30266.1"
                     /translation="MSRYFGYRPSGTVRTAVESFESTTQVRSVGRVLLGTVYVDIRET
                     EWAVAVAYGRTHHPKIREPELVYEVRYAYRTGDGTQVTKLDTQEDSPRKISAESFPSV
                     DAFVLWALNEESGRIRGTNN"
     gene            complement(9450..9761)
                     /locus_tag="PKM87_09130"
     CDS             complement(9450..9761)
                     /locus_tag="PKM87_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transport protein CbiN"
                     /protein_id="HNQ30267.1"
                     /translation="MSSKYGLEIIALIAIIGFCGLFLYTSSIMSDAEFAGSDTVGSSQ
                     IAELTGKAEEEFQPLIWQWSPPSGEIEAGIFALQAAIGGILVGWVFGYWKGQKKRDTT
                     E"
     gene            complement(9761..>9939)
                     /locus_tag="PKM87_09135"
     CDS             complement(9761..>9939)
                     /locus_tag="PKM87_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HNQ30268.1"
                     /translation="SSFALFMGIFAITQVPLAIVEGVVLALVFKYIVQLKPDIILRLK
                     VFPEEKIRAARGEA"
BASE COUNT         2211 a         2895 c         2610 g         2223 t
ORIGIN      
        1 acatacaggt caccggacac agtgccggtt tgtgtggttg agagcggaag ggtcgcagcc
       61 gatacagctc caggaattgc aaggagcaac aagagaataa aaaggctcat gcttgtcatt
      121 tgtttcatta ttcagtactc caaccgcggg tggcattccg cggatataca aaaaatcagg
      181 agtggctacc ataaatatat tatgattttt ttctatattc ttcacacaaa attcaatata
      241 tcaaaaacat aatatcatct tatatacaaa attaagaaaa ttgagttaaa cattacatga
      301 aaaagtatac gatcatgata tttcataaat ataaagatac tttttctgcc acctgggacg
      361 ttttcatcgc caagatcctg cataggaaaa agaaccaaga attgaggaat ttgaacatac
      421 ccatcacttc tctcacctca ttccaggtca tgccgggaac accgcgtgca ccgccttctc
      481 ctgtccgggt gtgatcacta cttcgaacgt ccgcgactcc ttctcgccta ccagcttcac
      541 ggtatagatg cctgtcgcca acgaaggtat gatgaccggg gtcttctcgc ccgtgtttct
      601 tccggagagg tagacctttg ctcccgcagg ggtgctcgtg aggtttaagc tgccatggga
      661 atacggttcg agcttaatga acaccttctc atcgatcggg ttgttcatgg catcgatcat
      721 cgagagttcc ttctctccag gaaggtatcc cggcgcctgg accgtgaccc ggtgcagccc
      781 gggcgacacg ttcgttacag cggccggtgt ccgaaggccg gtcgaaaacc cgtcaaagag
      841 gatatcgctc ccgggggggt ccgaagagat gaccacggta agagactgtg aatctgcagg
      901 ttcggaccgg atcaccatcg tgtcaccgga ttccagctgg ggaagcacgt tccgcgaaat
      961 atacgagcca tcgtggagga tcacgataaa agccggggtg ccctcagctt cggcctcccc
     1021 aggcatttca acaggcggcc ctctcccatt gaccgtgaac gctgagcccg cgtagtcctc
     1081 tgatgtgata ttcagcatcc gggcaaacat cgcaccctcc atgaacgaga tcatcgtgat
     1141 gctgcctctg gctacgtaga tcctccgggc agccgggaaa attgtgttct tcttatccat
     1201 ccctaccgtg tggtaccctt cacggagccc ccccaccacc cgcggggtga cctgatcggt
     1261 ccttcgccca tctatcagaa tggtcgcctc tgacgggtac gactcgacat agactcctcc
     1321 gagctttccg tttcctgatg gggaggatgt cgaaacaacc ggctttttcg ggacgtaggg
     1381 ggtgaatatg atacgcagat cgtaatctcc tgccgtcttc agggaggcgc ggtaggggtc
     1441 atacccgtcc agcgtgactg tcacttcctg tgtcccgccc cgtggaaccg ggcaggtaag
     1501 cggggtcagg tcttcatact caacgccgtt taccgttacc aatgccccct caggatcggt
     1561 gatgaccgtc aggttgacct cctctgatag cgccacgaca ggtgctatcg gtcccaggga
     1621 ttgtgcggaa gaaggcatga tcggcgcaaa aatccgctgg aacatccatc caaagaaccc
     1681 cgtatcctgc tggacgggtg tgagagtgac gatgacctcg gtctctgccg gtacagcagt
     1741 ccccgtcacc tggactggcg ctgtcctggt ggtcgtggga gccgcgggaa ggccggcatc
     1801 tcccggcacg accacgagca cagccccccg gttctccagg tagtcccccc gtttcagggt
     1861 gaggtaactc tggatcgtgg cctcgttaga gcctggacgg atgctttcca gtacatcgac
     1921 catggcgatg ctggtcgccg atgaaccgtt cacgagcagg ttgtcccatt ccgccccgtt
     1981 gaaggtgatc cggttctcgt catcaggctc tccggatgct gcagtgctga tcaaaaagag
     2041 atgtgccgtt gatgcaaacg ctgggacctc ctcaaaggga aagacggtgg tcgcatcttc
     2101 ggtcgtggtc tcatactccg gcccggccag cagcacatcg ctcccttctg cgatccagta
     2161 gagggtggga gtaccccctt cagtctcggt ggcaagtatg aggagcagtc catagaccgc
     2221 aaatgcctca tctcctttcc cggcattccc caccgaaaag gtgtaattcc ctgtctcctt
     2281 caccaggtcc tgtatatcgt aggaaaaggt cgttgcagga taatcatata tgccaaaacc
     2341 cttccgatcc gaatagtgtg cagccggaga gataggggta ctttcgtact gcagggtaag
     2401 aggagcggcg agcccctctc gtttcttctc atcatgatcc caggtcgtgt agacatagag
     2461 ccgagccagg cgcagctcat cacccgtccg aaggggcact tgcagggtat agttcttcga
     2521 ctcccctgaa aggatcaggt cggaatagtc gctcgtctcg tagaggttca cgctcccgtt
     2581 caccatcccg gtgagtaata ccgggatggt ttggccatag tacccgtttt cagccgtagc
     2641 gacctccccg gtatcagaac tgtcggagac cgtgctgcca gcgggggttg gcgttggctc
     2701 ctcctcatcg ggaggctctt ccggttcggt cccctgccgg atggtgaggg tcgcgaggat
     2761 tatcttgtag aactggcctt cagagcccga accggaacct ccataccggt cataggtgag
     2821 caggttatcc ccatcgctct ttacttcccc ggtcacatcc cactggttga atccgaaata
     2881 cgccccctgg ttctggcccc cctcaaggtc ctcaccattg aaacggtaat acccatcctg
     2941 gctggccagg tggttgacag aaagcgtggc cttctcgacc gaatcaaaaa tccctgaagg
     3001 atcaaacgag gtctcacccc ggtaatcctc cccaagctgc tcatctgaat aatacgtgtc
     3061 ggaatcatgc ccctggttca ccgcgtacca gacctggtcg gaatccccgt catcataggc
     3121 gaccaccagc gtgacagcct tgatcctccc gtcaaggctc gggttctgtt ggatggtctc
     3181 cacccgggcg cccgggtttc tggaggtgat cttcgagccc acgtcatacc acatcaggta
     3241 gtcgctcgtt acccggttca catggctgtt caccatgaca tacccgggcc ctccatcata
     3301 cacgtaggtg aagggaacgt ccaggttctc tgtgccaagc accgtctcgt acgtcccatc
     3361 tccatccccg tcgaaggaga tctttgccgt tccgttgtag ttgttctgca tgtgcccgca
     3421 atagaccgcc acgtacaatc gcgcccagcg gacctgggtg aagacaggga gcgagaactt
     3481 ctttgcgata tcccgctgcc cccatgaagg gaccatcgca tcaataaaca ggtcgccagt
     3541 caccatcccg gactggaccg tctcaaacgg cattcccccc acgtagggat cagccgatcc
     3601 ctgtgcctgg agcatcagca gaaggatcag aacccctgag atcagggcca gggaacccgg
     3661 aagattccat cctttctttc tgtttccatc catcttgttc atccacgacc acctttccgt
     3721 cttcatccag atctcacccg ccggaccgta ttgcctccac cggattgagc cgcgctgcct
     3781 ttactgccgg atataggccg gagagcacgg cgagcaggac cgagaccacg agccctgcga
     3841 taagaagccc gggagttacc cgggcaatct cggttccaaa caacagggta aactcctctc
     3901 ccaggcgtga gaggatgaac gaacgccctg cggtgttgac aagggcggca aacaccattc
     3961 cgcagaagca ccccaggacc cccccggcga ttccgatgat aaggcattcg gccaggatca
     4021 ggcggatgac atccctccgg gatgccccga cggccatggt aatcccgatc tcccgtaccc
     4081 gctcgtacac cgaggtcatc atcgtgttca tgatcatcaa cgccccgacc acgagcgaaa
     4141 taacggcaaa gagggcgagg agcaggacca ccatatccat caaccggttc accgattcaa
     4201 tctgctcaaa tgccccggtc gctccaagct gcatccctgt gatcgcagcg gcaacgccgg
     4261 ccacgtgctc gggggaatca accctgacga gaatcccgtc atacagtggt gcatcggtcc
     4321 gtgctgccac gacagctccc cggttggcaa ggaggatctc gtcaatcgaa tccccccgct
     4381 cctggagcac tccgaccaag gtgagctcaa tctcagaatc cagcggcttc cctgatgcat
     4441 cgtactcccg tatggtaacc acgaaacggt cattttgccg gatcccttcg taccgctgga
     4501 gtttctcgcc gaggcggtat cccgccacca cttcgcgggt ttccggtgag aacggcctcc
     4561 cccgggagta gcggaggttg aggagtccgc cctcttcgac agaatcaatc ccggttaccg
     4621 ggatgaacgt ttgccgggcc gttgcatacg tatcccgcgt aattccttct gccccggtga
     4681 cagaagagag gttccgaatt tccatgatcc gtgcgccggt aatgagttgc accacatccc
     4741 cgtgcacttt cggctggacg gtgatcaggg tcaggtcgga ctgggtcttg atggtctcga
     4801 ctgcattgta ccgtatcccc tccccaagcg agacagtggc gatcaccgct gcaaccccga
     4861 tggtaatccc gagtacggtc aggaaaaatc gcagccgctt cctccagaga tgccggaacg
     4921 caatggagaa aacatcgctg atcatgccac cacctgccca tcaaggagct cgatgcttcg
     4981 gtccgagtag ccggccaggt cccgctcatg ggtgaccacc aggaaggtga tgccctgatc
     5041 agtgttcagc tcacggagaa gacgataaat ttcccggctg gtcttcgaat cgaggtttcc
     5101 agttggttca tcggcaagca cgatcggggg attggctgcc agtgcccggg cgattgccac
     5161 ccgctgctgc tcaccccctg atagctcggc cgggtagtgg ccggtccggt cagcgagccc
     5221 gaccagactc aggagttcaa gggcccgctc ctgtcgctcc tgtcgggggc gcccgtggaa
     5281 gaggagcggg taggccacgt tatcggcagc agtcatggca ggaaggaggt tgaactgctg
     5341 gaagacaaaa ccaaccatct cccgcctcag tcggaccagt gcatgatggt tatggtaatc
     5401 cgtcagggct cccgaaatcc gcacgttccc tgacgtgggg tgatcaagac ctccaataat
     5461 attgaggagg gtgctcttgc cacttccgct cctccccaca atcgtgagaa actccccagg
     5521 agcgacagag aacgagatat ctcgaagagc actcactgtt tcggtatacc tctttgagac
     5581 tgactctacc tcaataatgc tgcgggtatc actcgtcata ggttacctcg agcaccgcat
     5641 tcgtgagctg gatatagtcc ccattgtcct ggacctccgc acggttcccc ccgggcctga
     5701 ggaagggctt tacctcccgt ctatcgactc cgacttgctg cagttcggag gaactgaaga
     5761 tatcggtcca tgttttccct gaaaatgagg ggaaaaggac gtgcatgacc ggccgtaaca
     5821 cgatggggac cacggcctcg tcaccccggt tgaagaagag acggttcatc tccgggacac
     5881 ccgagagcga gtatccaccc gagggggcga ccaggagcat ctcggcatcg tggacctgct
     5941 caagggagat agtaccggga tactcgatcc ggcttgttgc catttccggg gtgatcccgt
     6001 atcggctgta gaggaggtcg gtcccctcca tgatctgaat ctgtttctgc tggcctccgg
     6061 gcacggtata taccgcaaga aatccgaccc cctggatggt gaagctcgtc ccctcctcgc
     6121 cagtgtttac gattcgtatc gtgaaaggtc gcgagaagtc catcgcggaa ggtatttcgt
     6181 aggtgtccat cccggaatag aaatcattct ggctggcaaa tcccttgttg tcggtatacc
     6241 ggttcctgaa gtgaagggca ccaccttctc ccgggggcac gagctggacg tccatccttg
     6301 ggtaaatggc agcctgcccc tttttactcc aggaccagta gatatacacc cgctggtaga
     6361 ggagatcgga gccctccggg atcccaattg aaagcactgt atcatagccc gaaccggcat
     6421 tgatactccc gctgtaggta ccgtttgcaa gagagaagag gtagtcacct ggtaccgttc
     6481 ccgtgtatgc caggttgagc gggtggtccc ccgcgtacgt ggcagcacca gggcagatca
     6541 gggtaacgac ggcaaggcag atgatactgc atgtggggag atcaggagaa ctcatagacc
     6601 cgctcatcac tgttattcgc ccggtccttc tcaggtattg tcccccgttc gtcggcaact
     6661 acccgaaggg tatgcctgcc ggccgtagta aacaagggaa ttgtgacggt gatcgccccc
     6721 cctgccgaaa caccctcggt cactgtttgc tctgcaactt tctccccatc aagatacagg
     6781 gagagcacga acgctggggc attacttccg ccggtattcc tgaccgtgac cgggatagga
     6841 aactccgaga ctccgctacc cgctccggca acggacggca ccaagaccag tgcgaagata
     6901 ccaatggtaa ggaagagggc ggggagcgtg acaggggacg gccttttcct cgccatccac
     6961 caggccagga caagcccccc cgcaatggcg ccagcgaagg gaaatggggc atttttcgga
     7021 ctcgtgccgc cagtcccggg acttccggga tcgccaagtg ataccgagag gtcgggcagg
     7081 ctccctacag taatagagcg ggacgccgca ttgttggagg tgtccgtatc ccccttgact
     7141 gtgacggtga cggtgtgatc accaggcgcg gcctgccagc tgccctccac ctcccgaatt
     7201 ccttcgtatc cgatgattac ctgtgtggtg gccacaggaa ctccatcgac agaaaatacg
     7261 acctcaaccg gagcggaaga cacgatgcca ttattcctga tgaccgcgct gatagtcgcg
     7321 gtctcccctt ggtatgcacc ctggatggtg atgggatcta ctgaaagttc ggggacgagg
     7381 gagtctccgt tctctgtgac gaccagcatg gcaaggaccg ggtgaatgta gtcttcacct
     7441 tcagacagcg cgctcgcagt gactattacc ccgtccccgt tcaggtcccg gcctcgttca
     7501 aagacaaccg tattgctctc tttgagaagc gaggtaacat caaagacttc aagatcagca
     7561 tatcgggtat caataccact ctttccgttc gcatcggatg accgctcgtt cccaatatca
     7621 cgggcaccag gaagatactc agattcaggg attgcaagga tgcccagatc ctgcccgttg
     7681 aaggtgatat aatcgggttg accacgtgtt gatgtgagga ggagggttgt cagctctgca
     7741 gaacgtattg ctccagaaga aggggccgag aaggttacgg cagagctgtc atgccgtgtc
     7801 ggattggcac ctgcccaccc ttccccatgg aggttctcgt ttccatctgc tatccagtac
     7861 tgggtgacgg ggccattttc cgattcgatc agggcaacta cgatgattcc gtataccctc
     7921 ccgtcaagtt tgttattcgg ttcaccccgg ctggtagtga cggtaatggt gttatcgccc
     7981 tggccaataa ctccggtccc atcgaaggat atccagtaca cgccattgcc ggactcgtag
     8041 acctgccggt ccatatcatc ctcgccgaac agctcgattt tttcctgttt cttcccattg
     8101 atatcgattt gggtccatcc ccgatatttt tcggtcccac cccagactcc cgtgtataca
     8161 cgtgcccaag ctggctggtg ttcaagggca aattcatgtg aataaggagg ggctgaaaga
     8221 ccgaggctcc aggaactcac cacttccccc cggatcgctc cctgagcagt aataccgagt
     8281 gggacacctt cgaaagagta catggcagaa catggtacgg aaaggaggag catgataacg
     8341 atgagaatga tcgtaatagg gagggcatac cgcatagcag atattctcta atctttgtta
     8401 tttatttttt tatttttaat acgaattaat cattatgtaa aattagaatg taaatgtccc
     8461 tttctggtcc cggtggacta ttaattgcat ctgctcgtcc aggcaacagg tctcaaggtg
     8521 ctcccatcag aatccatttc ctctcataag gaagattctc cttttctttc atcaaggaaa
     8581 caagagtccg tcacgataat tccctagcct cgtgcagctt tttttaagaa aatacaagca
     8641 gattttacct ttttcaaatt cagttgagga aacatctatc ttcttggaga cagtatcagg
     8701 aaaccggtga tcactatgga atgcccaagc actgccaaac tttcgggaac ccagcttgat
     8761 gagatcagga aacttgaagg gaagttgggg gttatccttg tcgcttatga caaggtccat
     8821 ccctacaaga agctcacaaa tgaagcgctt tcaaaaatcc agtctgtcga aaaggatacc
     8881 gggtcaatcc taatcgcgta cgaggcctga taatccactc ttttccttgg atggcatccc
     8941 cgggatagta ttccaggcgt accgcttttc tggcccgagt tctgtgatgt aactctggtc
     9001 tgttttaccg gttccagtcg aaacacgtct cctgtttaat tgttggttcc cctgatccgc
     9061 ccgctctcct cgttcagggc ccacaggacg aatgcatcaa ccgaggggaa ggactctgca
     9121 gagatcttcc tgggcgaatc ctcctgcgtg tcaagtttcg tgacctgtgt accatccccg
     9181 gtccggtagg catatcgaac ctcgtagacc agttccggtt cccggatctt cgggtggtgc
     9241 gtccgcccgt aggcaacggc aacagcccat tccgtctcac ggatgtccac gtagacggtc
     9301 ccgagcagca ctctcccgac actccggacc tgggtggtcg attcaaacga ttccacggca
     9361 gtcctcacgg tgccggatgg acggtagcca aaatatcgtg acattccatc acttcttact
     9421 ccaaatatct gccatttttg atacactgct tattccgtag tatctctctt cttctgaccc
     9481 ttccagtagc caaaaaccca gccgaccaat atcccgccaa tggcagcctg aagtgcaaag
     9541 attcctgctt caatctcacc acttggcgga gaccactgcc agattaaggg ttgaaattcc
     9601 tcttccgctt tccctgtcag ttctgcgatc tgggaggacc ccacagtatc agacccggcg
     9661 aattctgcat cggacatgat tgaactcgtg tagagaaaca ggccacagaa tccaatgatg
     9721 gcgatgaggg cgatgatctc cagcccgtat ttggaggaca tcaggcctca ccccgggctg
     9781 ccctgatctt ttcttccgga aatactttca gccggaggat tatatcgggt ttcaactgga
     9841 caatatactt gaataccagg gcgagaacca ccccttccac aatggcgaga ggaacttgcg
     9901 tgattgcaaa gatccccatg aagagggcga aggaactca
//
LOCUS       DAOQYR010000081         9907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_7315_length_9907_cov_8.724320, whole genome shotgun sequence.
ACCESSION   DAOQYR010000081 DAOQYR010000000
VERSION     DAOQYR010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 9907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9907
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7315_length_9907_cov_8.724320"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /gene="nadE"
                     /locus_tag="PKM87_09140"
     CDS             <1..383
                     /gene="nadE"
                     /locus_tag="PKM87_09140"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNQ30269.1"
                     /translation="HANRDRRLVCGTSNRTEYLLGYSTKFGDSAADIQPIIHLYKTEV
                     CALAETMDLPERVMRKPPSAGLWPGQRDEEEIGLTYSEIDTALAALSRSGWKSRTPVE
                     EKVLGMVRASEHKRLPPPGLIPPD"
     gene            complement(367..1065)
                     /locus_tag="PKM87_09145"
     CDS             complement(367..1065)
                     /locus_tag="PKM87_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HNQ30270.1"
                     /translation="MENWREIAGTPELRYAEDLRAVLQDRECHCRGPVYSMYRDLAAT
                     TKDREWMASLAIRFDITCIPPATLCGEWVKTKGHYHPENFAGLGYPEIYQVFEGEAHY
                     LLQKRDLSEVILVEAGEGDTVVVPPGFGHVTINPGQRELVMANLVSGEFSSEYGFYEA
                     HQGAAYYELSDGRLEKNPQYPSLPHPIRVQAAAFLPGSPFTGPLYDLVMERDRRLECL
                     NHPERFGEFFSRAG"
     gene            complement(1065..2282)
                     /locus_tag="PKM87_09150"
     CDS             complement(1065..2282)
                     /locus_tag="PKM87_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HNQ30271.1"
                     /translation="MADVKYLTGDPDLSASVAGRNEFSPGEDVVLKVNIENRGLIDMK
                     FVRSGIVERDDLPSTAKLVKVGLSPGDSPIVIRSDPQLVGDIPGGKTASVSFSVKVPA
                     DAAAGNYTIPLQVHYVYLKTAEQFGQDAISYTYRDVDETLDLPIRIKPRAMLEAVSIS
                     SEHLNAGTEGYLQIAIRNRGSEDAKDAVVRIARNGNSPVIPTDSSLFVGEFPAGSSRE
                     ARFKVSVSRDAGEGDYPIDLSCQYTNSEGDVVTSDILTIGVPVGGKIQFSVVSSPAEV
                     HAGRKAVIEVEYQNTGSALARHAQARISAVDPFTSNDDTAYLGDLAPGEKAVARYEVH
                     VDPDATRKEYGLDSEIRYRDSLDNSAISDTMKVRVSVVEGENPYLLVAVVLVILAFAG
                     GAGYYILKRRKEQ"
     gene            2406..3179
                     /locus_tag="PKM87_09155"
     CDS             2406..3179
                     /locus_tag="PKM87_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ30272.1"
                     /translation="MAETPPSHLSLTESLKALGLTKYEALVYIGLLKVSGATATDIHE
                     ISGVPRASVYPVLDRLIQKNLVSVSHSTPKRFEAVPPEDGIDHLLELVESDAARARKM
                     LSRIYRDRSSGERGDQELIWSIYGDEHIRSRLIDLLQHAECSIDTIFFGGFLREEITA
                     ALIAKSGAVPIRVVTDRWDVPLPGKISVHVKSPPESLKNLKPSSVAGGVFLIDQRRAM
                     VVMVSGGEGPTALYSESKGFVKFFSLYWELLSSCEAVLA"
     gene            complement(3176..3922)
                     /locus_tag="PKM87_09160"
     CDS             complement(3176..3922)
                     /locus_tag="PKM87_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF128 domain-containing protein"
                     /protein_id="HNQ30273.1"
                     /translation="MNLPLKFVHTRIEEYAMQVTFDPREGRGTVVYNLSLIRKEDLEY
                     AISVMKDAFKAGICVSGLVRFIEEGATVGDFQVPSGHCAIITMCSSTLDGVLLRKGVP
                     SNPIGGGVVEIESRVPRRFTHMILYEHTTIDPLQALISQEITSINEMMRRGSGNILAN
                     IRQCHMEAETLVGEVIDDLAGSSFSGILDVGLPNTPALGVTVDPQYMGIVALGGTNPM
                     AAIKEGGVYVVTQAIKGLMDVQEMQEILEF"
     gene            3988..4959
                     /locus_tag="PKM87_09165"
     CDS             3988..4959
                     /locus_tag="PKM87_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30274.1"
                     /translation="MDEKVREKARNAIKSILEAAGFSVNTLEDPFDLAERDDEYLMVL
                     CSNDPGEIEEYNTGAYRLMKDGEGITYKKLLFSLEPSATAGNCILWRSDEFIRYAGEA
                     ALDEILGRPLALSFSDGSRTKADAGPALMRGRSTQAIEEEEPGGIVISHLPLRVQKQA
                     AEQIAKVQGQASLKFMPYWLYSYQSSGEQVYRDQRIGFDSEGSGGINAINGTKMDADW
                     NAVAESAIPQSAEVVQPRLTREEATEKVVAEVIESLTRKIRIKQVIGDAISYEEKVMR
                     PDKKNVVVQLQQAFIPVWQVRGKKIVEVNAYTGEVLSEPMDEGVEVF"
     gene            4972..6315
                     /locus_tag="PKM87_09170"
     CDS             4972..6315
                     /locus_tag="PKM87_09170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNQ30275.1"
                     /translation="MIAVLGGGPAGRIAAMQLGLAGREVTLVEKGGLGGQCLHHGCMV
                     VCALNDIARHLFFSRDLQSLGILDSVPTVSFSRTLAEMALVQAKIASVLDAETRSCGV
                     QVMQGSTGRVDGNSLYVDGELFSAEKIVVATGSLPRIPDIPGIRLQGVFTPHTLPSIK
                     SVPEHLLIIGGGIMAAEFAFIFREFGADVTLAARSTFLKGMHPRLRELAGRDLSGVTI
                     HEGCTVGRLEGDGAVSGAMLKTGAKENTVSCDGVFLAAGLVPNSGMVSGLKKGQDGRI
                     LVDRSMVTSNPSVYACGDVTGSRALTPVARYEGTVAAASILGREPPPDCTPIPQSMNL
                     ASEYAFVEADGSDDEVAFGTPGPAGPGTFWSVPRGHTGMAGILVSRESGKVGAVYTAS
                     PAAGILAAYHSFLMRLGVTAHDFKWFMEVHPMTDGVYPLMKYASFRLHQEKEDRP"
     gene            complement(6335..7939)
                     /locus_tag="PKM87_09175"
     CDS             complement(6335..7939)
                     /locus_tag="PKM87_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNQ30276.1"
                     /translation="MAAKPYPRMVTFGCGQVECVPAEEFGNKAANLATIASLGIKVPP
                     GFCLGVSLCEEYFESGRQLPPDLPALLATGISRLEKATGLSFGSSRRPLLVSVRSGAP
                     VSMPGVMETVLNIGLSRDTVRGLISLSGNPKFAWDSYRRLIQNFGQVVSRHPSALYQS
                     VLREEMERERVADEIELDAHTLRDLAAEYERIFSQNSGGKFPASAMQQLEQATEAVIK
                     SWESPRAEAYRRLNLIREARGTAVAVQAMVFGNLGSRSGAGVAFTRNPWQGNAQLLVD
                     FKFGAQGEDVVSGDQSISTQHEFQRALPETFLELSETGKKLETYYRDMQDLEFTVQNG
                     ELFLLQTRSGKRSPYAALKVAVDLCQEGIISPQDVIWMLREVDLDAISVERVRTRDHP
                     LAVGIPASGGVAAGDIAFSGASAEAMESEGRSVILIRETPSPDDIPGIRAAIGLLTAR
                     GARTSHAAVVARQMGRVCVVGCESLVIDEARRRCRISGQELREGARITLDGNSGKVFR
                     GEVEYSSERPTALIAMVREWEQSMHL"
     gene            complement(7955..>9907)
                     /locus_tag="PKM87_09180"
     CDS             complement(7955..>9907)
                     /locus_tag="PKM87_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30277.1"
                     /translation="EETMPAKKEIIEELGEEGLILPTLVNNALAANDRIKYLFTLLQA
                     ARSRAENPHADFSSLRVEREAAGIETTVYDRVVAESESAGEGVVRIPHAAQLFGEVRS
                     NLLEMATPLLSRDDDDAHTIRERLQALEQRIPTGEGDLAEKAAIDAITEGQSGDGDSV
                     HLLVMDMHRALNRLQGDLASEVIDGAMTYLLDEGDQEIVRAFMSGVRRTAPLKFDHPG
                     LGTTATRNGPRLILQNDIGMTDAHVLVINIEGLTATIIYTDIHMPRLQFFQGLFEGKG
                     VRWGDTLSKKGAQTFEKKIYHLSTGTYTASGNDDLARFLSFAGSRLVFLIDWNRARKR
                     LRNFLLTKDAVSVLKWAADNDVGHMGFLLLGGEKMIYDAMEMSSRVPMRYGEPLHQIL
                     GREKSIEYFQWVLRVSATGLLANKSRLLVQDEIKAELLRYFRSAHENLMEICEEHATL
                     TISVATVVREALQHIQMGGDVSFIARSAQRAKKWESEADLAVTKVRTLSRRIENADFY
                     SALIFTADDALDFLEEASFFVTLIPSVTFSRKINQSLVEMAELAERSAQEFLKALIAS
                     QYVYKGYSRDDMQEFLQAIDHVLSLEHQSDEALRLAQKTILVESRDWKEMQVYGDLAR
                     TIEESTNSLMKAAFLLRDDIMERMNR"
BASE COUNT         1886 a         3060 c         2928 g         2033 t
ORIGIN      
        1 accacgccaa ccgcgacagg aggcttgtct gcgggacctc caaccggacc gagtatctcc
       61 tcggctactc gaccaagttc ggggacagcg ccgccgatat ccagccgatc atccacctct
      121 acaagaccga ggtctgtgcg ctcgctgaga ccatggacct gcctgaacgg gtgatgagga
      181 agcctccctc ggcagggctc tggcccgggc agcgggacga ggaggagatc gggctcacgt
      241 acagtgagat cgatacggcg ctggccgctc tctcaaggtc agggtggaag agccgtaccc
      301 cggtggagga gaaggtgctt ggaatggtca gggcaagcga gcacaagcgc ctccccccgc
      361 cgggtctcat cccgcccgac tgaagaactc cccgaaccgt tcggggtgat tgagacattc
      421 cagtcggcga tccctctcca ttacaaggtc atagagcggg cccgtgaaag gggatcccgg
      481 caggaatgcc gctgcctgta cccggatggg atgagggagt gaaggatact ggggattctt
      541 ctctaacctg ccatcggata gttcgtaata cgcagctccc tggtgcgcct cgtaaaatcc
      601 gtattcactt gaaaattcgc ctgatacgag gtttgccatc accagctcgc gctgcccggg
      661 gttgattgtc acatggccaa agccaggggg cacgacgact gtatcgcctt ctcctgcctc
      721 caccaggatg acttccgaga ggtcccgctt ctgaaggagg taatgggcct ccccttcgaa
      781 tacctggtag atctccgggt atccaagtcc cgcaaagttc tcggggtgat aatgaccctt
      841 ggtcttcacc cactccccac acagggttgc aggagggatg caggtgatat caaaacggat
      901 cgcgagggag gccatccact cccggtcttt cgtggtggct gcaaggtccc ggtacatcga
      961 gtagacaggg cctctgcaat gacactccct gtcctgcagg acggccctga gatcttcagc
     1021 gtagcgcagt tctggggtcc ctgcaatctc ccgccagttc tccatcactg ttcctttcgt
     1081 ctcttcagga tataatatcc cgctccgcct gcgaaggcaa ggataacgag gaccaccgcg
     1141 acaaggagat acgggttttc tccttccacg acactgacac gcaccttcat cgtgtctgat
     1201 atcgcgctgt tgtcaaggga gtcgcggtag cggatctcgg agtcgaggcc atactccttt
     1261 cgggttgcat ccgggtcaac gtgtacctcg tacctggcga ccgccttttc gccgggggcc
     1321 aggtcgccga ggtaggcagt atcatcgttg ctggtgaagg ggtcaaccgc actgatccgg
     1381 gcctgtgcat gcctggccag tgctgatccc gtgttctggt actccacttc tatgaccgcc
     1441 tttctccctg catgtacctc ggccggggag gagacgaccg agaactgtat ctttcctccg
     1501 acagggacac cgatggtgag gatgtccgat gtgaccacgt ccccctcact gttggtatac
     1561 tggcaggaga gatcgatcgg atagtccccc tcgccagcgt cacgggagac cgagaccttg
     1621 aatcgtgcct ccctgctgct tcctgcaggg aactccccca cgaacaggct gctgtcggtc
     1681 gggatcacgg ggctgttgcc gttccttgca atcctgacca ccgcatcctt cgcatcctcg
     1741 gacccccggt tccgtatagc gatctgcagg tacccttccg ttccggcatt gaggtgctcg
     1801 ctgctgatgg agaccgcctc gagcatcgcc ctcggcttta tccggatggg aaggtcaagt
     1861 gtctcgtcaa catccctgta ggtataggag atggcgtcct gcccgaactg ttccgcggtc
     1921 ttcaggtaca cgtagtggac ctgcaggggg atagtgtaat ttcctgccgc ggcatccgcg
     1981 ggcactttga cagaaaagga gacggatgcg gttttcccac cggggatgtc ccctactaac
     2041 tggggatcag acctgataac aatgggcgag tccccagggg agaggcccac cttcacgagc
     2101 tttgcggtgc tgggaaggtc atcccgctcc acgattcccg acctgacaaa tttcatatcg
     2161 atcagccccc tgttctcgat attgaccttc aggacgacat cctcgccagg ggagaactcg
     2221 tttcttccgg ccacggacgc ggagaggtcc gggtccccgg tcaggtactt gacatccgcc
     2281 atgaccggcg ctccaattaa actaaataaa atggccagaa tcacgaaatg acgcatattc
     2341 atgtgaaacc acttgttgtt attgctataa caacatttaa gattcgccat atataatggt
     2401 atgtaatggc cgaaacaccc ccatcccatc tctccctcac ggaatcccta aaagccctcg
     2461 ggctcaccaa gtacgaagcg ctggtgtata tcggtctcct caaggtctct ggagcgactg
     2521 ccaccgatat ccacgagatc tcaggcgttc cccgtgcctc ggtctatcct gtgctggacc
     2581 gcctcatcca gaagaacctg gtctcggtct cccactccac ccccaagcgg ttcgaggccg
     2641 ttccccctga ggacggaatt gatcacctgc tggagttggt cgagtcggat gccgcacgag
     2701 cgaggaagat gctctcccgg atctacaggg acaggtcatc aggtgagcgt ggagaccagg
     2761 agctgatatg gagcatatac ggcgacgagc acatccgctc ccgcctgatc gacctcctcc
     2821 agcatgcgga gtgctccatc gacaccatat tttttggcgg attccttcgc gaagagatta
     2881 ccgcggccct gatcgcaaag agcggggccg tccccatccg ggtggtgacg gaccggtggg
     2941 acgttcctct ccccgggaag atctcggtgc acgtgaagtc cccgccggag tccctgaaaa
     3001 atctcaagcc ctcgtctgtt gccgggggag tgtttctcat tgaccagcgg agggccatgg
     3061 tggtcatggt ctcgggggga gagggaccga ctgcgctcta ttctgagtca aaaggattcg
     3121 tgaagttctt ctcgctttac tgggagctcc tctcctcgtg cgaggcagtt cttgcctaaa
     3181 actccaggat ctcctgcatc tcctggacgt ccatcagccc cttgatcgcc tgggtcacga
     3241 catagacgcc cccttccttt atggcggcca tcgggtttgt ccctccaagg gccacaatcc
     3301 ccatgtactg cgggtccacc gtgaccccga gcgccggggt attcgggagg cccacgtcca
     3361 gtatcccgga gaaactgctg ccggcaaggt cgtcgatcac ctctcccacc agggtctcgg
     3421 cctccatgtg gcattgccgg atgttcgcaa ggatgttccc actccccctt ctcatcatct
     3481 cgttgatcga cgtaatctcc tgggagatga gtgcctggag cgggtcgatg gtggtatgtt
     3541 cgtacaggat catgtgggtg aaccgccttg ggacacggct ctcgatctcg accaccccac
     3601 ccccgatggg gttcgagggc acgcctttcc tgagaaggac cccgtcgagc gtggagctgc
     3661 acatcgtgat gatggcacaa tggcccgatg gcacctggaa atccccgact gttgccccct
     3721 cctcgatgaa acgcacaagg ccgctcacgc aaatccctgc cttgaacgcg tccttcatga
     3781 cagagatggc gtattcgagg tcctctttcc tgatgagcga gaggttgtag acgacagtcc
     3841 ctcttccttc ccggggatcg aaggtcacct gcatggcgta ctcctctatc cgggtatgca
     3901 cgaacttgag ggggaggttc atctctccat ctaatctctc ctgtcaatag aaaaattgtt
     3961 ctataagatg ggggaaatgt atggttaatg gacgaaaagg ttcgggaaaa ggcgagaaat
     4021 gcgataaagt ccatcctgga ggcggcaggg ttctcggtga acacgctcga ggatcccttt
     4081 gacctggccg agcgggatga tgagtacctc atggtcctgt gcagcaatga cccgggagag
     4141 atcgaggagt ataacaccgg tgcataccgc ctgatgaagg acggcgaggg tatcacctat
     4201 aagaagctcc tcttctccct cgagccttct gccactgcgg ggaactgcat tctctggagg
     4261 agcgacgagt tcatccggta cgcgggagag gcggccctgg atgagatcct tggacgcccc
     4321 ctcgcactct cgttttcgga tgggtcgcgc acgaaggcgg atgcggggcc cgcgctcatg
     4381 aggggccgca gcacccaggc catagaagag gaagagcccg gggggattgt catctcccac
     4441 ctccccttgc gggtgcagaa gcaggctgcc gagcagatcg cgaaggtcca ggggcaggca
     4501 agcctgaagt tcatgccata ctggctctac tcataccaga gttcagggga acaggtgtac
     4561 agggaccagc gcatcgggtt tgattccgag gggagcggcg ggatcaatgc catcaacggg
     4621 acgaagatgg atgccgactg gaatgccgtc gcagaatctg ccatccccca gagtgccgag
     4681 gttgtgcagc cccgtctcac cagggaagag gctaccgaga aggtcgttgc agaggtgatc
     4741 gagagcctca cccggaagat ccggatcaag caggtgatcg gcgatgccat ctcgtacgag
     4801 gagaaggtga tgagaccaga caaaaagaac gtcgtggtgc aattgcagca ggcattcatc
     4861 cctgtctggc aggtgcgggg caagaagatc gtcgaagtga atgcctatac gggcgaggtc
     4921 ctttccgagc ccatggacga gggcgtcgag gtcttctgat atggccggtc catgatcgca
     4981 gttctcggcg gaggccccgc gggcaggatc gcagccatgc agcttggcct cgcaggaaga
     5041 gaggtcaccc ttgttgagaa gggggggctc ggggggcagt gcctccacca tggctgcatg
     5101 gtggtatgcg cattgaatga catcgccagg cacctcttct tctccaggga ccttcaatct
     5161 cttggcatcc tcgactctgt ccccaccgtg tcattctccc ggaccctcgc cgagatggcc
     5221 ctggtgcagg caaaaattgc gtcagtcctc gatgccgaga caaggagctg cggcgtccag
     5281 gtcatgcagg ggagcacggg aagggtggat ggcaattccc tttacgtgga cggcgaactg
     5341 ttttcggccg agaagattgt cgttgccacc ggctcgctcc cccgcatccc cgatattccc
     5401 ggtatccggc tccagggggt attcactccc cacaccctcc cctcgataaa gagtgtcccg
     5461 gagcacctcc tgatcatcgg cggtgggatc atggcagcgg aatttgcctt catcttcagg
     5521 gaattcgggg cagacgtcac tctcgctgcc cgaagcacct ttcttaaagg tatgcacccg
     5581 aggctccggg aacttgcggg aagggatctc tcaggggtga caatccacga ggggtgcacg
     5641 gtcggcaggc tggagggaga tggcgcggtc agcggtgcca tgctgaaaac aggcgcaaaa
     5701 gagaacacgg tttcctgcga cggggtcttc cttgccgcgg gactggtccc gaactccggc
     5761 atggtctccg ggttgaaaaa agggcaggac gggcggatcc tcgtcgaccg ctcgatggtc
     5821 accagcaacc cctccgtata cgcatgcggg gacgtgaccg gctcacgtgc gctcacgccc
     5881 gtggcacggt acgaaggcac ggtcgccgca gcgtcaatcc ttggccggga acctccccct
     5941 gactgcacgc ccatcccgca gtccatgaac cttgcatcgg aatacgcatt cgtggaagcg
     6001 gacgggagcg atgacgaggt cgcatttggg accccggggc ccgcggggcc ggggacgttc
     6061 tggtcggtcc cacgggggca cacggggatg gcgggtatcc tcgtctcgag agagtcagga
     6121 aaagttggcg cggtgtatac cgccagtcct gctgcaggga tcctcgctgc ctaccactcg
     6181 ttcctgatgc gcctcggggt caccgcccat gacttcaaat ggttcatgga ggtccacccc
     6241 atgacagacg gagtatatcc cctgatgaag tatgcctcgt tccggcttca ccaggagaag
     6301 gaagatcggc cctgaccggg tcaaacgctg ccgctcacag gtgcattgac tgttcccact
     6361 cccgcaccat cgcgatgagc gcggtggggc gttcagacga gtattcgacc tcccccctga
     6421 acaccttccc cgaattgccg tcgagggtga tccttgcacc ctccctgagt tcctggccgc
     6481 tgatccggca ccgccgcctc gcctcgtcga tgaccaggct ctcgcacccc accacgcaga
     6541 cccggcccat ctgccttgcc accaccgcgg catgagaggt cctggcgccc ctcgcggtga
     6601 ggagaccgat cgctgccctt atccccggga tatcgtcagg agaaggggtc tccctgatca
     6661 gtatcacgct cctcccttcc gattccatcg cttcagcgct ggcgccggag aacgcgatat
     6721 cccctgctgc aaccccgccc gacgcaggaa tcccgactgc gagggggtgg tcccttgtcc
     6781 tcacgcgttc gacagagatc gcatccaggt caacctcgcg gagcatccag atcacgtcct
     6841 ggggtgagat gatcccttcc tggcagaggt ccaccgcgac cttcagcgcc gcgtaggggg
     6901 agcgtttccc gctcctggtc tgcaggagga aaagctcccc gttctggacc gtgaactcga
     6961 ggtcctgcat gtccctgtaa taggtctcga gcttttttcc ggtctccgag agttccaaaa
     7021 aagtctctgg cagggcccgc tggaactcgt gctgggtgct gatcgactgg tctcccgaga
     7081 ccacgtcctc accctgtgcc ccgaacttga agtccaccag cagctgggca ttcccctgcc
     7141 agggattcct ggtgaaggca actcccgccc ccgagcgcga acccaggttg ccgaagacca
     7201 tggcctgcac tgcgaccgcg gttccccgtg cctcccgtat gaggttgagt cgccgatacg
     7261 cctctgcccg gggactttcc catgacttga tcactgcctc ggtcgcctgc tcgagctgct
     7321 gcatggcgct ggcaggaaac ttcccgccac tgttctgtga aaatatccgt tcgtactcgg
     7381 cggcaaggtc gcggagggtg tgcgcgtcca gttcgatctc atctgcgact ctctcccgct
     7441 ccatctcctc gcggagcaca gactggtaga gcgcagaggg gtgccgggac accacctgcc
     7501 cgaagttctg gatgagcctc ctgtaggagt cccatgcgaa tttcgggttc cccgagaggg
     7561 agatgaggcc gcgcaccgta tcgcgcgaaa gcccgatgtt caggaccgtc tccatcaccc
     7621 cgggcatgga gactggcgcc cctgaacgga ctgagaccag gaggggcctc ctgctgctgc
     7681 caaacgagag gcccgttgcc ttctccaggc gggagatccc ggttgcaagg agggcaggca
     7741 ggtctggcgg aagctgccgt ccggactcaa aatactcctc acagaggctc acccccaggc
     7801 agaacccggg gggcaccttt attcccaggg atgcaatggt ggcaaggttt gctgccttgt
     7861 tgccaaactc ttctgccggg acgcactcca cctggccgca cccgaacgtg accatgcggg
     7921 gatatggttt tgctgccatc acctcttcct cacctcatcg gttcatcctc tccatgatgt
     7981 cgtcgcggag gagaaatgcc gccttcatca gggagttcgt ggactcctcg atggtccggg
     8041 ccaggtcgcc atagacctgc atctccttcc agtcccgcga ctccacgagg atggtcttct
     8101 gggcgaggcg gagggcctcg tccgactggt gctcgaggga gaggacatgg tcgatcgcct
     8161 ggaggaactc ctgcatgtca tccctgctgt accccttgta gacgtactgc gaggcgatca
     8221 gcgcttttaa gaactcctgg gcactcctct ccgcgagttc ggccatctcc acgagcgact
     8281 ggttgatctt cctcgagaac gtgacggacg ggatgagtgt cacaaagaag gaggcctcct
     8341 ccaggaagtc aagcgcgtca tccgcagtga atatgagggc cgagtagaag tcggcattct
     8401 cgatcctccg ggagagggtc cgcaccttgg tgactgcgag atccgcctcg ctctcccatt
     8461 tcttcgccct ctgtgcgctc ctcgcgatga aggagacgtc tccccccatc tggatatgct
     8521 gcagcgcctc cctgacaacc gttgccacgc tgatcgtaag agtcgcgtgc tcctcgcaga
     8581 tctccatcag gttctcgtgc gccgaccgga agtagcggag cagttctgcc ttgatctcgt
     8641 cctggaccag gaggcgcgac ttgtttgcga ggagaccggt cgcggagacc cggagcaccc
     8701 actggaaata ctcgatactc ttctcgcgcc cgaggatctg gtggaggggt tccccgtacc
     8761 gcatggggac gcgggatgac atctccatcg cgtcgtagat catcttctcc ccgccgagaa
     8821 gcaaaaaccc catgtgcccg acgtcgttgt ccgcggccca tttcaggacc gaaaccgcgt
     8881 ccttggtgag gagaaagttc cgcagcctct tccgggcgcg gttccagtcg atcaggaaga
     8941 ccaggcgcga ccctgcgaag gagaggaacc tcgcaaggtc atcattcccg gacgcagtat
     9001 acgttcccgt tgagaggtgg tagatcttct tctcgaaggt ctgcgctccc ttcttcgaga
     9061 gcgtgtcgcc ccacctgacc cccttgccct cgaagagtcc ctggaagaac tgcagcctcg
     9121 gcatgtggat gtcggtatag atgatggtcg cggtcaggcc ctcgatgttg atcaccagca
     9181 cgtgggcatc ggtcatcccg atatcgttct ggaggatcag tcttgggccg ttcctcgtcg
     9241 cggtggtccc aaggccgggg tgatcgaact tcaggggtgc agtgcgccgg acccctgaca
     9301 tgaaggcacg gacgatctcc tggtccccct cgtcaaggag gtaggtcatg gccccgtcga
     9361 tcacctcgct tgcgagatcg ccctggagcc tgttcaaggc acggtgcatg tccatcacca
     9421 ggaggtggac gctgtcgccg tctccgctct ggccctcggt gatcgcgtcg attgctgcct
     9481 tctccgcgag gtctccctcg ccagtaggaa tgcgctgttc aagggcttgc agtcgttccc
     9541 tgatcgtatg agcatcgtcg tcgtcgcggg acaggagggg tgtcgccatc tccaggagat
     9601 ttgacctcac ctccccgaag agctgcgcgg cgtgggggat gcggacgacc ccttctcctg
     9661 ctgattccga ttcggccacc accctgtcgt agacagtcgt ctctatcccc gccgcctccc
     9721 tttccacccg caggctggaa aaatctgcat gcgggttctc ggccctggac cgggctgcct
     9781 ggagaagggt gaagagatac ttgatccggt cgttagccgc aagggcgttg ttgaccaggg
     9841 tgggaaggat cagcccctcc tcaccaagct cttctatgat ctccttcttt gcaggcatgg
     9901 tttcctc
//
LOCUS       DAOQYR010000082         9861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_7356_length_9861_cov_7.722415, whole genome shotgun sequence.
ACCESSION   DAOQYR010000082 DAOQYR010000000
VERSION     DAOQYR010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 9861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9861
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7356_length_9861_cov_7.722415"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1620)
                     /gene="ileS"
                     /locus_tag="PKM87_09185"
     CDS             complement(<1..1620)
                     /gene="ileS"
                     /locus_tag="PKM87_09185"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNQ30278.1"
                     /translation="MKEVSSSYQAQEIEESVQKYWREENTYGKVKELHKEGKDFFFVD
                     GPPYTTGHIHLGTAWNKIIKDCMLRYHRMSGRNVIARAGYDMHGLPIEVRVEQTLGFS
                     SKKDIERFGIAAFIDRCRTFADTNKDIMSEQFMHLGVWLDFDDPYQTMHPGYIEAAWW
                     TLKRADEEGMLERGFRVVNWCPRCATAIADAEVEYWDERDPSIFVKFPVHGSEGEYLV
                     IWTTTPWTLPANVAVAVHPEFTYARVEAVKEGKKEVLWIAEDLVEPVLRKGRYQDYKV
                     IETCSGREMCARTYHSPLRDQVPLQAEISHRVVTADFVAMENTGMVHIAPGHGWDDYL
                     LGCKEDLPIICPVDAQGHFTGDAGVLSAMFIKDADQVVLRELGEHLLASDHVVHRYGH
                     CWRCKTPIIFRATSQWFLKASLLKDRMLEEVGKVTWYPDWAGSARFYDWIKEARDWCI
                     SRQRYWGIPIPIWTCGSCGTHRVIGTIAELEDASGSPVLDPHRPWVDGVEIPCTCGGT
                     MRRVEDIFDVWFDSAVASWATLGFPREKEALK"
     gene            1968..3425
                     /locus_tag="PKM87_09190"
     CDS             1968..3425
                     /locus_tag="PKM87_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNQ30279.1"
                     /translation="MPGRGNDGVPEHGCIIGGEERYSGEIIEVRFPYTGELVARVHQA
                     TPGDLDNAIRMAHLGFAKTRELSSTERYRILTRLATIVESRAEELANLMVLEGGKTIS
                     FARAEVQRAAETLRISAEEAKRIGGEVISLDWTPGNEGRFAITRRMPIGVVLGIIPFN
                     FPLNLACHKAGPAVAAGNPIILKPSSATPVSALLLGEMIVSAGFPREAVSIVPCPGDR
                     AGRMACDPRVAFVSFTGSPEIGWKIRETAGRKPVSLELGGIAPVIVHEDANLPYAVSR
                     ILTGGFTNAGQVCISVQRVFLHRAIYHDACRMICEGTAAIATGDPRLPETVVGPMISS
                     EAADRALAVVRDAEAQGARVRVGGRVEGSLFYPTVISDTAPTTRVNSGEIFAPVITLT
                     PYDTLKEAIDSANGVAFGLQMGIFTQNIQRIIKTFSLAEYGGVQVNDIPTFRVDHMPY
                     GGVKGSGLGREGPRYAIEEMTVPRLLAFNPAGGVE"
     gene            3428..3883
                     /locus_tag="PKM87_09195"
     CDS             3428..3883
                     /locus_tag="PKM87_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HNQ30280.1"
                     /translation="MQEPAAQVAVIGPSECTEDEAAAAAVIGGVISAHGATLISGGLG
                     GVMEASCRAAKENCGLVVGILPGYGEGNPSLSVSIRTGLSHARNFLVVESSDAIVAVG
                     GAYGTLSEIAIALKRGKPVFGYRSWDIEGVQPCKTPEEAALRALSAARR"
     gene            complement(3855..5708)
                     /locus_tag="PKM87_09200"
     CDS             complement(3855..5708)
                     /locus_tag="PKM87_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HNQ30281.1"
                     /translation="MVKRYLSVVSLEKALSIVGEVPIPLSPETIPLELSAGRITARPI
                     FATYSVPEVHLAAMDGIAVRSADTFGASEQTPVTLPAARRVNTGNVVPAEYDAVIMIE
                     DVWTEGDFYTIRKPAGPWQHVRPAGEDIAESEMVVPSHHRIRPSDIGALAAYGIVSVE
                     ILGFRVGLVPTGSELVPPGSKPAPGQVVESNTRMAEAWLSQVGGKCTRYPLTPDVPER
                     IRDAISRAVEENDLVIVSAGSSAGTRDYTAETIAGLGEVLVHGVAIKPGKPVIIGKIQ
                     SKPVIGMPGYPLSALTVLREIVAPLVRLSGIPVPEPAVIAARLTATLHSEAGSDEFVL
                     LAAGRIGSSWVATPLSRGSGVQMSAVRSNAYLQIISSLEGYEAGEMVPVSLTVSAPEA
                     EEAILVTGSHDPVLDHLAELAKRDHVEVHSNHVGSMGGIIALKRNDCHAAPIHLLAPD
                     GDYNIPYLMKYLPREEVELICVAGREQGIVSQNGISIDDLPGTSFINRQKGSGTRVLL
                     DHELRLREISPGDIPGYEREATTHIGVALAVKTGEAEAGMCVYSAAKALGLPFVPVAK
                     ERYELAIRREHMEDPRIRSLLDAIRSPHFREVLGRLGGYDDSITGVQRKVP"
     gene            complement(5702..6907)
                     /locus_tag="PKM87_09205"
     CDS             complement(5702..6907)
                     /locus_tag="PKM87_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNQ30282.1"
                     /translation="MSLFLRTVPVEDAIRVAAGIAPAPSCVEIPLEEAGGRVLSRDVV
                     ADIDIPCFDRSVMDGYAVRASDTIGAGEAIPSLLSLCGTVKMGRASDITLSAGECAYI
                     PTGGQLPAGADAVVMIEHTEQIDDQVLVKRPVAPGENLIRKGEDFARGMPVLKKGRRL
                     SPPDIGVLAAVGCHTVPVCTIPSIGVISTGNELIPVRDIPTGSQIRDSNSGMLGTYLR
                     RVGCTETLYGIVPDERAALKDALEKALSENDGVLLSGGSSKDERDLCTDIIGELGEVL
                     VHGVSIAPGKPTIIGRCREKPVLGLPGHPASALVVLDRIGRPLLAAMSGEVMGKGCTR
                     TALLAQNIPSIRGREDYVRVRLEGDRAFPLFGKSGLLNTLVQSDGVVRIPAGSEGIEI
                     GETVEVLLW"
     gene            complement(6904..7080)
                     /locus_tag="PKM87_09210"
     CDS             complement(6904..7080)
                     /locus_tag="PKM87_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30283.1"
                     /translation="MDEEKIRKAFEAEGITKEIKCPQAFAMSEKYGIAKLDIAKYCNT
                     HGVKIRACQLGCFK"
     gene            complement(7223..7909)
                     /locus_tag="PKM87_09215"
     CDS             complement(7223..7909)
                     /locus_tag="PKM87_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30284.1"
                     /translation="MSSSTRIFIVFLVTGCCLAILPAGAQAPIGGDRAWYAVHCNVDG
                     ASVFFDGDYKGDIQGGVLYVEAYTTGTPYRTYSVQKEGYSTFSDSIDGVPGKGETFDL
                     YATLNPTQPTQSPVIGGDIGWYTVHCNVNGASVMFDNTYQGAITNGILTVQVYTTGTP
                     YSTYTVTMDGYLPYTAPITSFPAKGQTVDLYATLNPAPTPTPTKSPVPAGIAFSALAA
                     AGLLALSRKK"
     gene            complement(8063..8647)
                     /locus_tag="PKM87_09220"
     CDS             complement(8063..8647)
                     /locus_tag="PKM87_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ30285.1"
                     /translation="MIDENNIDSLPLHIRTEPPFYADFTANPVSGNPPLTVQFTDASD
                     GNIMRYLYRFGDGFSSMSQNPVYTYRRPGNYTVSLTIWQMDGRTLSSKTMVKENYIRV
                     EGAPGPDVRTNFSATPVSGTAPLQVAFTGNSSGSPILWKYSFGDGFMSTQKNPTHTYR
                     RPGTYTVKLTVWTIGPDRKLATETIERASYITVA"
     gene            8799..>9861
                     /locus_tag="PKM87_09225"
     CDS             8799..>9861
                     /locus_tag="PKM87_09225"
                     /inference="COORDINATES: protein motif:HMM:NF016312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNQ30286.1"
                     /translation="MKIALVSSLADPGGCTIHGSLLSLLATPHEPYSLERHQLSHHRV
                     RERLIYQDHLDRDLDADLIIFLSRHSSVNPVPVLTVHVTGNISSAEFGGQERSLAPAA
                     PEWMHAVLRELSGNAPPGFRVAYEVTHHGPSELATPSLFAEVGSSEKEWRDSAAGMAV
                     ARSVLSADPADCIPLIGFGGTHYAARQTHIALNTRGAFGHIAHTREVSSLDAGMIDQM
                     REKTRAVAAYIDRKAIPGRDLARLEDLLAERRVKILSEGELMHIGDLSWETYMRVLSL
                     AEQIVPGCRVTLHGQSPGGEPVRIDLDPVLLEEAWRLNQKGFLAGLDTLPLVRLSTQK
                     RPVWPSFITIGENSGNVLHD"
BASE COUNT         1971 a         2920 c         2943 g         2027 t
ORIGIN      
        1 cttgagcgcc tccttctctc ttgggaaccc gagagttgcc catgatgcga ccgcggagtc
       61 gaaccagaca tcaaagatgt cctcgactct ccgcatggtc ccgccgcacg tgcagggaat
      121 ttctaccccg tccacccatg gccggtgggg gtccaggacg ggcgaaccgc tcgcatcctc
      181 gagttcggcg atagtcccga tcacccggtg cgttccgcac gacccgcagg tccagatcgg
      241 gattgggatc ccccagtaac gctggcgtga gatgcaccag tccctcgcct ccttgatcca
      301 gtcatagaag cgtgcgctcc ctgcccaatc aggataccag gtgactttcc cgacctcctc
      361 gagcatgcga tccttcaata gcgatgcctt caggaaccac tgcgaggtag ccctgaagat
      421 gattggtgtc ttgcagcgcc agcagtggcc ataccggtgg accacgtggt ctgatgcaag
      481 gaggtgctca ccgagctccc gaaggacgac ctggtccgcg tccttgatga acattgcgga
      541 gagcaccccc gcgtccccgg taaaatgacc ctgtgcatcg accgggcaga taatgggaag
      601 gtcctccttg catccaagaa gatagtcgtc ccatccgtgt cctggtgcga tgtggaccat
      661 cccggtgttc tccatggcaa caaagtccgc ggtaaccacc cggtgggaga tttccgcctg
      721 gagcggcacc tgatcccgga ggggagagtg gtaagtcctg gcacacatct cccttccgga
      781 acatgtttcg atcaccttgt agtcctggta cctgcctttc cggaggaccg gttcaaccag
      841 gtcttctgcg atccagagca cctccttctt cccttccttg accgcctcca cccgtgcata
      901 ggtgaattcc gggtggaccg cgactgcgac atttgcaggg agtgtccagg gggtggtagt
      961 ccatatgacg aggtattccc cctcactgcc gtgcacgggg aacttcacga atatggaggg
     1021 gtctcgttcg tcccagtact ccacctcggc atccgcgatc gcagtggcac agcgtggaca
     1081 ccagttcacc acccggaacc cccgctcgag catcccctct tcatccgccc gtttcagggt
     1141 ccaccatgcg gcctcgatat agcccgggtg catggtctgg tatgggtcat cgaagtcaag
     1201 ccagacaccg aggtgcatga actgttcgct catgatatcc ttgtttgtgt cagcaaaagt
     1261 gcggcagcgg tcgatgaatg ccgcgatccc gaaacgttcg atgtccttct tagaggagaa
     1321 gccgagtgtc tgctcgaccc ttacctcgat tggcaggccg tgcatgtcgt agcctgccct
     1381 tgcaatcacg ttcctcccgc tcatgcggtg atagcggagc atgcagtcct tgatgatctt
     1441 gttccaggcg gtaccaaggt ggatatggcc ggtggtgtaa ggggggccgt ctacgaagaa
     1501 gaaatccttc ccctccttgt gcaactcctt gaccttcccg taagtgttct cctccctcca
     1561 gtacttctgg accgactcct cgatctcctg ggcctgataa ctgctgctca cttccttcac
     1621 acgcatcccc ctttcctgtc gcttcgccgg gtggagagat agacagtcct gtaatatggg
     1681 ctgttctttc caaagtagtt tggggagcct tccggatatg cattgcagaa agcgcccggc
     1741 tcccctctta tgtcctgttg ccccggccat ccctgcaatt gaatgcgggg gaccgaggtg
     1801 cattcacgtt cagagaataa aacgatcagt catggaatat cccatcacgt aggtataggt
     1861 gggagaggat ggtataaaaa agtggacggt tcttttcaag agcccggatg taaagatgcg
     1921 ggaccttttt ctctggcaag ggagcacctt gtggcaggag catgagcatg ccaggacgtg
     1981 gaaatgacgg ggtgccggag cacgggtgta tcatcggcgg agaagagcgg tattcggggg
     2041 agatcatcga ggtcaggttc ccgtataccg gcgaactggt ggcaagagtc caccaggcga
     2101 cccctggtga tctcgataat gctattagaa tggcgcattt gggatttgcg aagacaaggg
     2161 aactctcttc aaccgaaaga taccgtatcc tcacgcgcct tgcgacgatc gttgagtcac
     2221 gggcagaaga actcgcgaat ctgatggtcc tggaaggggg aaagaccatc tcctttgccc
     2281 gggccgaggt gcagagggcg gcggagaccc tccggatatc cgcagaggag gccaagcgga
     2341 tcggaggcga ggtcatctcc cttgactgga ctcccggaaa cgaggggagg tttgccatta
     2401 ctcgccgcat gcctatcggg gtggtgctcg ggataatccc cttcaacttc cccctcaacc
     2461 tcgcctgcca caaggcggga cctgcggttg ccgcagggaa cccgatcatc ctgaagccgt
     2521 catcggcgac ccccgtctct gcgctcctcc ttggagagat gatcgtttcg gcaggattcc
     2581 cgagggaggc cgtaagcata gtcccctgcc cgggtgatcg cgccggccgg atggcatgcg
     2641 atccacgtgt cgcattcgta tcgttcacag ggagcccgga gatagggtgg aagatccggg
     2701 agaccgcggg gagaaagccg gtctcactcg agctcggcgg catcgcgcct gtcattgtcc
     2761 acgaagatgc aaacctccct tacgcggtct cgcgcatcct caccgggggt tttacgaacg
     2821 cggggcaggt ctgcatctcg gtgcagcgtg tgttcctgca ccgtgccatc taccacgatg
     2881 catgccggat gatctgcgaa ggaacggcag cgattgcaac aggcgacccc aggcttcccg
     2941 aaaccgttgt cgggcccatg atcagcagtg aggcagcaga tcgggccctc gcggtggtcc
     3001 gcgacgcaga agcccagggc gcccgcgtcc gtgtcggcgg gcgcgtggag gggtcgctct
     3061 tttacccgac cgtgatatcc gacacggcgc ccacaacaag agtaaattct ggggagatct
     3121 ttgcccccgt gataacgctc acaccttatg atacgttgaa agaggcgatt gattccgcga
     3181 acggagtcgc gttcggtctc cagatgggaa tatttaccca gaacatccag cggatcatca
     3241 aaactttctc ccttgcagaa tacgggggcg tccaggtaaa cgacatcccc acgttcaggg
     3301 tcgaccacat gccatacggg ggcgtgaagg gatcgggcct ggggcgggag gggccgaggt
     3361 atgccatcga ggagatgacc gtgccaagac tgcttgcctt caacccggcc ggcggggtgg
     3421 aatgaggatg caggaaccgg cggcgcaggt cgcggtgata gggccctcgg aatgcacgga
     3481 agacgaggcc gccgctgcag ccgtgatcgg gggcgtgatc tcagcccacg gggcaaccct
     3541 catctcaggc gggctcggcg gcgtgatgga ggcttcctgc agggctgcaa aggaaaactg
     3601 cggcctcgtg gtgggtatcc tccctgggta cggcgagggg aacccctccc tctcggtctc
     3661 gatccgcacc ggtctctccc acgccaggaa cttcctcgtt gtcgagagtt cagatgccat
     3721 tgtggcggtg ggaggggcct acggtaccct ctcggagatc gccattgcgc tgaagagggg
     3781 aaagccggtc tttggctacc ggtcctggga catcgagggg gtgcaaccct gcaaaacccc
     3841 ggaagaggcg gcgctcaggg cactttccgc tgcacgccgg tgatggaatc gtcgtagccg
     3901 cccagccgcc caaggacttc cctgaaatgt ggcgacctga ttgcatccag gagcgaccgg
     3961 atgcgggggt cctccatgtg ctcccggcgg attgcaagct cgtaccgttc ttttgccacc
     4021 ggaacaaagg ggaggccgag tgcctttgct gccgagtaga cgcacatccc tgcctctgcc
     4081 tcaccagtct tgactgcgag ggccaccccg atatgggtgg tcgcctcccg ttcatagcca
     4141 gggatgtctc cgggtgatat ctcccgaagc cgcaattcgt ggtcgaggag cacgcgggta
     4201 cctgatcctt tctgcctgtt gatgaacgaa gtcccgggga gatcgtcaat agagataccg
     4261 ttttgggaca caatcccctg ttctctcccc gctacgcaga tgagttccac ctcctcgcgg
     4321 gggaggtact tcatcaagta ggggatatta tagtcgccat ccggcgccag caggtggatg
     4381 ggcgcggcgt ggcagtcatt cctcttcagg gcaatgatcc ctcccatgct gcccacgtgg
     4441 ttggagtgga cctcgacatg gtcccttttt gcgagttccg cgaggtgatc gaggaccgga
     4501 tcatggctgc cggtgactag gatcgcctcc tctgcctccg gggcactgac tgtcaggctg
     4561 accgggacca tctcgcccgc ttcgtacccc tcaagagacg agatgatctg gaggtaggcg
     4621 ttcgaccgga ccgcgctcat ctggacaccc gatccccggg agaggggagt tgccacccag
     4681 gaggagccga tgcgtccggc tgcaagaagg acaaactcgt cacttcctgc ctcagagtga
     4741 agagtcgcag tgagcctcgc ggcgatgacg gccggctccg ggacggggat accggagagg
     4801 cggacaaggg gagcgacgat ctcccgaagc acagtaaggg cagagagcgg gtaccccggc
     4861 atgccgatca caggcttgga ctggatcttc ccgatgatga ccggctttcc cggcttgatc
     4921 gccacgccgt ggacaagcac ctcgccgagg cctgcgatcg tctccgcggt gtagtccctg
     4981 gtcccggccg atgacccggc cgagacgatc acgaggtcgt tctcttctac tgcacgggag
     5041 attgcatccc ttatacgttc cgggacgtcc ggagtgagcg gatagcgggt gcactttccc
     5101 ccaacctgtg agagccatgc ctctgccatc cgggtattgc tctccaccac ctggccaggg
     5161 gcaggcttcg aaccgggagg aacgagctcg ctcccggtgg ggacaagccc aaccctgaaa
     5221 ccgaggatct cgactgacac gatcccatag gctgcaagtg cgccgatatc agaaggacgt
     5281 atccgatggt gggaagggac gaccatctcg gactcggcaa tgtcctcccc cgcaggccgg
     5341 acatgctgcc aggggccggc gggcttccgt atcgtgtaga agtccccttc agtccacacg
     5401 tcctcgatca tgatcaccgc gtcatactcc gcaggcacca cgttcccggt attcacccgg
     5461 cgtgctgcgg gtagcgtgac cggtgtctgc tcacttgccc cgaacgtatc ggcactccgg
     5521 acggcgatcc catccattgc ggccaggtgg acctccggaa cggagtacgt ggcaaatatg
     5581 ggccgtgccg tgatccggcc ggcagacaac tcgaggggga tcgtctcggg agagagaggg
     5641 atgggaactt ctccgacgat cgagagggct ttttcaaggg agacgacaga gaggtaacgc
     5701 ttcaccagag gagcacctcg actgtctcac caatctctat accctcgctc ccggccggaa
     5761 tcctcaccac gccgtcgctc tggacgagtg tgttcaggag acccgacttt ccaaagagcg
     5821 ggaatgcacg gtctccttcc agcctgaccc gtacgtagtc ctcgcgtccg cgaatagatg
     5881 ggatgttctg ggcaagcagt gctgtcctgg tgcatccctt ccccatcacc tctccagaca
     5941 tcgccgcaag gagaggtctg cctatcctgt caagcacgac cagcgcagat gcagggtggc
     6001 ccgggaggcc gagcaccggc ttttcgcggc atcgcccgat gatggtcggc ttcccgggag
     6061 caatcgacac gccatggaca agcacctccc caagctcccc gatgatgtcc gtgcagaggt
     6121 cgcgttcgtc ctttgagctt ccccccgaga ggaggactcc gtcgttttcg gagagcgcct
     6181 tctcaagggc atccttcaac gcagcacgct cgtctggcac gatcccgtac agcgtctccg
     6241 tgcagccgac tcgccgcagg tatgtcccca gcatcccgct gttgctgtca cgtatctggc
     6301 tgccggtggg gatgtcccgg acaggtatga gttcatttcc ggtcgagatc acccctatag
     6361 aggggatggt gcaaaccggg acggtatggc aacccacggc cgcgaggaca ccgatatccg
     6421 gtgggctgag gcgacggcct ttcttgagaa ctggcatgcc ccgggcaaag tcctccccct
     6481 tcctgatgag attctcaccg ggggcaaccg ggcgcttcac cagcacctgg tcgtcgatct
     6541 gctcggtgtg ttcgatcatc accaccgcat ccgcccctgc agggagttgc ccgccggtgg
     6601 ggatatacgc acattctcca gccgacaggg tgatgtcgga ggccctgccc atcttcaccg
     6661 ttccgcatag agagagaagg gaggggatag cctcgccagc cccgatggtg tctgaagccc
     6721 tgaccgcata cccatccatc acggaccgat caaagcacgg gatatcgata tctgcaacga
     6781 cgtcgcggga aagcaccctt ccccccgcct cctcgagcgg gatctccacg catgaaggcg
     6841 ccggggctat ccctgcggca acccgtattg catcttcgac gggaacagtc cgtaaaaaaa
     6901 ggctcatttg aagcagccta actgacaggc ccttatcttc acgccatggg tattacagta
     6961 tttggcgata tcgagctttg caatcccgta tttctcggac atggcaaacg cctgggggca
     7021 tttgatctcc ttggtgatgc cttccgcttc gaaggccttg cgtatcttct cttcatccat
     7081 gcaggtacct atggagggtg acagtaataa atgagttgac ttcttccggg ttgttaagct
     7141 ccatgagtgg atacgatcag ctgaacacat tccataatag cctggaagtc aggccttttg
     7201 ttgaaacgaa aagaaaaatg agttattttt tcctggacag tgcgaggagc ccggctgccg
     7261 ccagggcaga gaacgcgatg cccgcaggga caggagactt tgtaggggtc ggcgttggag
     7321 cggggttcag cgtggcgtac aggtcaacag tctgcccctt tgcagggaaa ctggtaattg
     7381 gcgcagtata gggcaggtat ccatccattg tgacagtgta ggtgctatag ggggtaccgg
     7441 tggtgtacac ctggaccgta aggatgccat tcgtgatggc cccctggtag gtgttgtcga
     7501 acatcacgct cgcaccgttc acattgcaat ggacagtgta ccacccgata tccccaccta
     7561 ttaccgggga ttgtgtcggc tgggtggggt tgagggtcgc atacaggtca aaggtctccc
     7621 cttttccggg tacgccgtcg atagagtcgc tgaaggtgga atatccttcc ttctggacgg
     7681 aatacgtgcg gtagggtgtc ccggtagtat acgcctcgac atacaggacc cctccctgga
     7741 tgtcaccctt gtaatcgccg tcaaagaaga cgctcgcgcc gtcgacattg cagtgcactg
     7801 cataccaggc gcggtctccg ccgatggggg cctgggcgcc tgccggcagg atggcaaggc
     7861 agcatcctgt aaccaggaat acgatgaaaa tacgagtaga gctgctcatt gtgatcgcct
     7921 ttttttgaat accacgggta tattcgatcc gttcatttaa aatggattcc atcggtgtga
     7981 cacaagaaaa ggcgattaat ccctgggagg gaattttagc gatgcagtaa cccgattata
     8041 aaaattggaa tgggagggtt tttcatgcca cggtgatata actcgctctc tctatggtct
     8101 ctgtggcaag tttcctatct gggccaatgg tccagaccgt gagtttcacc gtgtaggtcc
     8161 ccggtctccg gtaggtatgg gtcgggttct tctgggtgga catgaacccg tcgccgaaac
     8221 tatacttcca gagaatcggg cttcctgacg aattcccggt gaaggcaacc tgaaggggtg
     8281 cagtgcctga tacaggggta gccgagaaat tcgtcctgac atcgggtcct ggtgcccctt
     8341 ctacccggat gtaattctct tttaccatgg ttttcgagga cagtgtccgc ccgtccatct
     8401 gccagatagt caggctcacg gtgtagttgc ctggccttcg gtaggtatac accggattct
     8461 ggctcatcga cgaaaatccg tctccaaacc tgtaaagata acgcatgatg ttcccgtcag
     8521 aggcatcggt gaactgcacg gtgagaggcg ggttcccgga gacaggattc gcagtgaaat
     8581 ccgcatagaa gggcggttcg gtcctgatgt gtagcgggag gctgtcaata ttgttctcat
     8641 cgatcacgaa gggtgcgttg cagaacccgt cagtccggac cggggcctgt tattgcgagt
     8701 ctattccctg tgcggtccca ttgctggatt gccacgatca cacaccccgg atcgcgtccc
     8761 tttatagaaa ttgaaaggca tagaatggga agtaaccaat gaagatcgca cttgtcagct
     8821 cccttgcaga ccccggcggc tgcaccatcc acggatccct tctctcgctc cttgccacgc
     8881 cacacgagcc ctattccctg gaacggcacc aactctccca ccacagggtc agggaacggc
     8941 tgatctacca ggatcacctt gaccgggacc ttgacgctga tctcatcatc ttcctctccc
     9001 ggcactcctc ggtgaacccg gtaccggtcc tgaccgtgca tgtcaccggg aacatctcga
     9061 gcgctgaatt cggcgggcag gaacgctcgc tggccccagc ggcccccgaa tggatgcacg
     9121 cggtattgag agagttgtca gggaatgcac cgccaggctt tcgtgtcgcg tacgaggtca
     9181 cccatcacgg cccgtcggaa ctcgcaaccc cctcgctctt tgcagaggtg gggagcagcg
     9241 agaaggagtg gcgggacagc gcggccggga tggcggttgc aaggagcgtg ctctcggcag
     9301 atcccgcgga ctgtattccc ctgatcgggt ttggcgggac gcactacgca gcccggcaga
     9361 cccatattgc cctgaataca cgcggggcgt tcgggcatat cgcccatacg agggaggttt
     9421 catccctcga tgccggcatg atcgaccaga tgcgggagaa gacgagggcc gtagctgcct
     9481 acatcgacag gaaagcgatt ccaggaagag acctcgccag gctggaggat cttcttgctg
     9541 aacgcagggt gaaaatcctc tcggagggtg aactcatgca catcggggac ctctcatggg
     9601 agacctacat gagagtcctg tcccttgccg aacagatcgt cccgggatgc cgagtcacgc
     9661 tccacgggca atccccaggt ggcgaacccg tgagaatcga cctggacccc gtgctccttg
     9721 aagaggcatg gaggctcaac cagaagggat ttctcgctgg gctggatacg ctcccgctgg
     9781 tccgtctctc gacccagaaa aggccagtat ggccttcttt catcaccatc ggtgaaaata
     9841 gcgggaatgt actgcatgat t
//
LOCUS       DAOQYR010000083         9223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_7995_length_9223_cov_7.954079, whole genome shotgun sequence.
ACCESSION   DAOQYR010000083 DAOQYR010000000
VERSION     DAOQYR010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 9223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9223
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7995_length_9223_cov_7.954079"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..668
                     /gene="psmB"
                     /locus_tag="PKM87_09230"
     CDS             27..668
                     /gene="psmB"
                     /locus_tag="PKM87_09230"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit beta"
                     /protein_id="HNQ30287.1"
                     /translation="MNERYQEGMKGTTTVGLVFQDGVVLASEMRATMGYLIASKKAKK
                     IYQIADRIGMTTAGGVGDAQQMARLMTVECSLYQIRRGRPMTVGAVATLLSNILNNNR
                     YYPFYVQLLVGGIDENGPSLYAVDALGGASKEEEMVATGSGSPMAFGVLEDRYQKDMT
                     EEEAVSLAVRAVRSAMKRDAGSGEGIHVVVITKEAYREIGDQEVAEHFPAIPA"
     gene            695..2584
                     /locus_tag="PKM87_09235"
     CDS             695..2584
                     /locus_tag="PKM87_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-CASP ribonuclease aCPSF1"
                     /protein_id="HNQ30288.1"
                     /translation="MLIEERLKELKNKINEKVPRGITVSEVEFEGPELVIYTDDPKKF
                     ADQADLIKILARDLRKRIVVRPNILEDPEKAVQDIRAVVAENAGITDIYFDADTGEVL
                     IEAEKPGVVIGKNGATLREITKHIGWTPKVVRTPPIESVTVKQIRQYLRSVNEERKEF
                     LRNIGRRIHRDVIARDQWVRVTMLGCCREVGRAAFLISTPESRVLVDCGEKPGNSAST
                     PYLYVPEIHPLTQLDAVVLTHAHLDHCALVPLLYKYGYDGPVYSTPPTRDLSAMLQLD
                     YLDVVSKEDRKIPYSSNEVKNYIKHSITLNYGSVTDIAPDIKLTFHNAGHILGSAIAH
                     FHVGDGLYNIAFTGDFNFSKSRLFNPATNTFPRLEALVMESTYGGSGDIQPSRADAEE
                     KLYETVKNVIQRGGKVIIPAFAVGRSQEVMLALEEAMRKEKIPRVKIYLDGMIREATA
                     IHTTYPEYLNSDLRTQIFKEGLNPFLAEYFAPVDSPDLREKVINGDPCVIITTSGMLN
                     GGPVMEYLSNLAFDERNALVFVGYQADGTLGRRIQKGWREVPIGRKDTIVINLEIVTI
                     DGFSGHSDRKQLVNYIGHIQPRPEKIFTVHGDENNTIDLASSLYKRFHIETHSPMNLE
                     TYRLV"
     gene            2581..3684
                     /locus_tag="PKM87_09240"
     CDS             2581..3684
                     /locus_tag="PKM87_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ30289.1"
                     /translation="MRARLPLFLGVFLVMALSNAIVPVLPSFAAGTTAQGAIFSAYFL
                     GAFLLVLPAGYLADRVGEVPLIRAGLLLTCVSGVMLSVAASPALVLAARLLEGFGAGL
                     FVPASLALLNARADHERMSGYFMALLNLGLLVGLLGTGWLVLQWGGVYAGIYLFTVAA
                     FIPLALSLGMRRGAAGQAGIPAETAAATLDRLKWVFSSYFWLWVSAVLIIGITGALTA
                     LHPEYTDLPPDLVSQEIALMNLATIGAILVTSHASLPPVPTIRAAAVAMACGVALTFF
                     SVWGFLVVGWLAGMVMISQLAFLAGASARQGVLMGLFNTASYGGMTLLPFFAGVIAEY
                     AGFPAAFAAIGLCALLVAATIGRCGCKVKAESP"
     gene            3681..5423
                     /locus_tag="PKM87_09245"
     CDS             3681..5423
                     /locus_tag="PKM87_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNQ30290.1"
                     /translation="MTLTPPVRSGTLLPGDRGDGRVVAFFDIGTNSIRLLVVRLYRDC
                     TYQVLTRQKEVVRLGEGEFDEHWINDGAMERAVVVCLRFIDLARSFGAEEFVAVATSA
                     TREAKNKHDLLHRLRQEAHLDVRVISGEEEARLIYLGVAGSTHLGEDRALFIDIGGGS
                     TELCLGGQHSYEVLTSLPLGSIRLTNQFFECDFDGPVSKKRYESICHHIREVLAPAIS
                     RFPRKKPGRVIGSSGTIINLGEIAARTATGPRSAGPWILDRKGLSRVIPLLCSLPLKE
                     RRNVPGINPERADIIIAGAAVLDCLMEAFSFDRIEITGRGLQDGLLADYLTRMEDFPL
                     LGQLNVRERSVLQLARSCGVNEYHARTVARLALELFDSSKDSGLHSLGDEERELLLYA
                     TFLHDVGSFISYQDHHVHSHYLISNSDMPGFSREEVLIMANIARFHRKKAPGKRELAG
                     TGLSTADQKRVRALTSMLRMAESLDRSHAALVRHAQFRHVKGERATLLVSAVGECQLE
                     MWGIEHEAGYFRRVFGKTLAIEVCRTELSEIREREDGLLPNLLNHSMSSGRERYSREG
                     DAGQGRKKAGRPGR"
     gene            complement(5515..6327)
                     /locus_tag="PKM87_09250"
     CDS             complement(5515..6327)
                     /locus_tag="PKM87_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNQ30291.1"
                     /translation="MIDLGILEYGFFQNALLAGLFASISCGIIGSYVVVRRMVSISGG
                     ISHAAFGGVGLGYLFGLDPLAGAFGFTTVIALGIGILKEKAGQNLDTLVAAVWASGMA
                     LGIICISLAPGYAPDLFSYLFGNILLVPGQDILLMAVLCAIILAVVAILYTQLQAVTF
                     DEEYARVMNLPVTRLSLLLLVLVAVTVVMLIRVVGIILVIALLTLPAATARGFTKRLH
                     TMMAASVCLGLLFSIGGLLLSYLLDIPSGATIILLATAGYVISLPITRAARG"
     gene            complement(6324..7094)
                     /locus_tag="PKM87_09255"
     CDS             complement(6324..7094)
                     /locus_tag="PKM87_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HNQ30292.1"
                     /translation="MIPAIEVRDVSFGQDGSPILDSVSLSVMEQDMYALIGPNGGGKT
                     TLLKIILGLLPARSGTVRIYGKSPGEGRQIVGYVPQFRTFDFRYPITVKEMVLSGRLS
                     HTRGMLRRYREEDHAAVRKVLERLDLSGLAGRTLSHLSGGEQQRAILARALAGNPRVL
                     ILDEPTVYVDTPTAFQFFDILDELKRSMTILLVSHDIGVISKHVTKVACLNHTLHTHN
                     TPEITEDMIAATYHCPVDLIAHGLPHRVFDDHGGEEGQ"
     gene            complement(7091..8074)
                     /locus_tag="PKM87_09260"
     CDS             complement(7091..8074)
                     /locus_tag="PKM87_09260"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNQ30293.1"
                     /translation="MTKGEGGTHGRTPGPARDGTVVMGAGAARLLWLSLLLVLAAMYS
                     AGCLDNGSENGSELQVAVTILPQEEIARAVGGEKVCITVVVPPGVEPHTYEPSAGQIA
                     RLSQADMYFRLGRGLLPFEDALVDRLLTLNPDLVVVDTSEGVSLIRVQGQDHGAMQGG
                     DPHVWLSPSNLRIMAGHMAEGFSIIDPGRAADYAAGKEAYIRRVDATDTNIRDMLSGM
                     EGKPFLVFHEAWGYFAREYGLVQVAVGAEGKEPGARELSSLITFARENGIRVVFAEPQ
                     YDTRGSEVIAREIHGSVVLIDPLAPDTLSNLERVASAISLIYGEGNGGAVP"
     gene            complement(8061..8492)
                     /locus_tag="PKM87_09265"
     CDS             complement(8061..8492)
                     /locus_tag="PKM87_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNQ30294.1"
                     /translation="MDQCDVAGLSPRKVAYLKYLYERDETARTGEIAAHFTVDPSTVS
                     KTVRELASTGLVDHRPYTGVVLTGKGHEYAEFLVRRHRILGLILTHYGLAGEEACREA
                     ERFESYVSRDAINKMCQSLGHPTRGICGAISHDRGCCVDEG"
     gene            complement(8565..>9223)
                     /locus_tag="PKM87_09270"
     CDS             complement(8565..>9223)
                     /locus_tag="PKM87_09270"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ30295.1"
                     /translation="VSLEVCGAGGCNLTRKEQFITVREKEPLTAGFLANTTSGAAPLH
                     VQFQDNSTGNPDSWSWDFGDGATSTEPSPCHLYPLPGVYTVRLVICAEGCMATADAVT
                     VVNVHPQVIGGSVGFFQVMCSAEGAHVFLDGRYMGAIENGTLNIPVYVTAAPARILQV
                     RAEGFLQYYTKIADYPGEGETIVLDVPLQPLSQEEAFFGRFQVPTLMGNLTISGPSLM
                     "
BASE COUNT         1905 a         2811 c         2761 g         1746 t
ORIGIN      
        1 gtttacgaat attttggggt gtgtgaatga atgagagata ccaggaagga atgaagggca
       61 ccaccaccgt cgggctggtg ttccaggacg gcgtggtcct cgcatcagaa atgagggcca
      121 cgatggggta cctgatcgcc tccaagaaag caaagaaaat ataccagatc gctgaccgga
      181 tcggaatgac cacggccggg ggtgtggggg acgcacagca gatggcacgg ctgatgaccg
      241 tcgaatgcag tctctaccag atcaggagag gcaggccaat gacagtcggc gcagtcgcca
      301 cactcctgtc caacatcctc aacaacaacc ggtactatcc cttctatgtc cagcttctcg
      361 tcggcgggat agacgaaaac gggccgagcc tctacgcggt ggatgcgctc ggaggagcat
      421 caaaggagga agagatggtt gccaccgggt ccggttcgcc gatggcattc ggcgtactcg
      481 aagacaggta ccagaaggac atgacagagg aggaggcggt atcgctggcc gtgcgggcag
      541 tacgctcggc catgaagaga gatgcaggct ccggggaagg aatccacgtg gtggtcatca
      601 caaaagaggc atacagggag attggagatc aggaagtcgc tgaacacttc ccggccatcc
      661 ccgcataact cctttttttt cgggtaggtt ttgaatgctt atagaagaga gactcaaaga
      721 gctcaagaac aagatcaatg agaaggtccc aaggggaatc accgtctcgg aggtggagtt
      781 cgaagggccg gaactggtca tctataccga tgacccgaaa aagttcgcag accaggcaga
      841 ccttatcaag atcctggccc gcgaccttcg gaaacggatc gtcgtccgcc cgaacatcct
      901 cgaggacccc gaaaaggcgg tccaggacat ccgtgccgtg gtcgcggaga acgcgggtat
      961 cacagatatc tattttgatg cggataccgg agaagtcctg atcgaggccg agaagcccgg
     1021 tgtcgtgatt gggaagaacg gggcgaccct ccgggagatc acgaaacaca tcggctggac
     1081 ccccaaggtc gtgaggactc ctcccattga gagcgtgaca gtcaagcaga tccgccagta
     1141 cttgcgttct gtcaacgagg agaggaaaga gttcctccga aacatcggga ggcgcatcca
     1201 ccgcgacgtg atcgcacggg accagtgggt cagggtcacc atgctcggct gctgccgcga
     1261 ggtgggaagg gctgcgttcc tcatctcaac cccggaatcg agggtcctcg ttgattgcgg
     1321 agagaaaccg gggaacagtg cgagcacgcc atacctctac gtgccggaga tccatccttt
     1381 aacccagctg gacgcagtgg tcctcaccca tgcccacctc gaccactgtg ccctggtgcc
     1441 cctcttatat aaatatggat acgatggccc ggtctatagc acccccccta cgcgggacct
     1501 ctccgcgatg ctccagctcg attacctcga tgtcgtcagc aaggaggacc ggaagatccc
     1561 ctacagctca aacgaggtca agaattacat caagcactcc atcaccctca actatgggag
     1621 cgtcaccgat atcgcccccg acatcaagct gacattccac aacgcaggcc atatcctcgg
     1681 ctcggccatc gcccacttcc atgtcggcga cggcctctat aacatcgcgt tcaccggcga
     1741 cttcaacttc agcaagagcc gcctcttcaa ccctgccacg aacacttttc cgcgccttga
     1801 agcgctcgtc atggagagca cctacggcgg gagcggcgat attcagccgt cccgggccga
     1861 tgccgaggag aagctgtacg aaacagtgaa gaatgtcatc cagaggggcg gaaaagtcat
     1921 tatccctgcg tttgcggtcg gcaggtcaca ggaggtgatg ctggctctcg aggaggcgat
     1981 gcggaaggag aagatcccgc gggtaaagat ctacctggac gggatgatca gggaggcaac
     2041 cgcaatccac acgacttacc ccgagtacct gaacagcgac ctcaggacac agatcttcaa
     2101 ggaggggctc aaccccttcc tcgcagagta cttcgccccg gttgactccc ccgatctccg
     2161 ggagaaggtc atcaacggag acccctgcgt catcatcact acgagcggga tgctgaacgg
     2221 cggtcctgtg atggagtacc tgagcaacct tgcctttgat gaacgcaacg ccctggtgtt
     2281 cgtaggctac caggctgacg gcacccttgg gcggcgcatt cagaaaggat ggagagaggt
     2341 gcccatcggg aggaaggaca ccattgtgat caacctcgag atcgtgacaa tcgacgggtt
     2401 ctccgggcac tcggacagga agcagctggt gaactatatc ggccacatcc agccgcgtcc
     2461 cgagaagatc ttcacggtgc acggtgacga gaacaatacg atcgaccttg ccagttcgct
     2521 ctacaagcgc ttccatatag agacccactc cccgatgaac ctggagacgt acaggctggt
     2581 atgagagcaa ggctccccct cttcctgggg gtcttcctgg tcatggcact ctcgaatgcc
     2641 atcgtcccgg tgctcccctc ctttgcggcc gggacgacgg cacagggggc catattttcc
     2701 gcgtatttcc ttggggcatt cctgctcgtc cttcctgccg ggtacctcgc cgaccgggta
     2761 ggagaggtcc cgcttatcag ggccgggctc cttctcacct gcgtaagcgg cgtcatgctc
     2821 tcggttgcag cctcgccggc gctcgtcctg gccgcacggc tgcttgaagg gtttggtgcg
     2881 ggcctctttg tccctgcgtc tctcgcactc ctcaacgccc gtgcggacca tgagcgtatg
     2941 agcgggtact tcatggcgct cctcaacctc ggcctgctgg tggggctgct gggcacgggc
     3001 tggctggtgc tgcagtgggg gggagtgtat gcaggcatat atctcttcac tgtcgctgcg
     3061 ttcatcccac tcgcactctc gcttgggatg cgcaggggtg cggccgggca ggcaggtatt
     3121 ccggccgaga ccgcggctgc caccctcgac cgcctgaaat gggtgttctc ctcctacttc
     3181 tggctctggg tctccgcggt cctgatcatc ggtatcaccg gggccctcac tgcgctccac
     3241 cccgagtata cggacctgcc gccggacctc gtttcccagg agatcgcact gatgaacctc
     3301 gcgaccatcg gcgcgatcct ggtcacctcc catgcaagcc tccctcccgt tcccaccatc
     3361 agggctgcag cggtggcaat ggcctgcggt gtggcgctga ccttcttctc ggtatgggga
     3421 ttcctcgtcg tcgggtggct tgcagggatg gtgatgatct cccagctcgc gttcctggca
     3481 ggcgccagcg cccgccaggg ggtattgatg gggcttttca acaccgccag ttacggcggc
     3541 atgacgctcc tccccttctt tgcgggagtt atcgcggaat acgcagggtt tcctgcagca
     3601 tttgctgcca tcggcctgtg cgcactcctc gttgccgcaa ccatagggcg ctgcggctgc
     3661 aaagtgaagg cagagagccc atgaccctca ccccgcccgt gcgctcagga acacttcttc
     3721 caggcgaccg cggggacggc cgggtggtcg cattcttcga tatcggcacc aactcgatcc
     3781 gcctcctcgt ggtccggctc tacagggact gcacctacca ggtcctgacc cgccagaagg
     3841 aggtcgtgag gcttggggaa ggggaattcg acgagcattg gatcaatgac ggggcaatgg
     3901 agcgggccgt ggtggtctgt ctgaggttca tcgatctcgc acgctccttc ggtgcggagg
     3961 agttcgtggc agtggcgaca tctgccacgc gggaggcaaa gaacaagcac gacctcctcc
     4021 accggctcag gcaggaggcg cacctcgatg tccgtgtcat ctccggtgag gaggaagcgc
     4081 ggctcatcta cctcggcgtt gcaggcagca cccatctcgg ggaggaccgc gcgcttttca
     4141 tcgatatcgg cggggggagc actgaactct gccttggggg gcagcactcg tacgaggtac
     4201 tcacctcgct cccgctcggg tccatccggc ttacaaacca gttcttcgaa tgcgactttg
     4261 acgggcccgt ctcgaagaaa cggtacgaat ctatctgcca ccacatccgg gaggtgctcg
     4321 caccggcaat ctccaggttc cccaggaaga agccgggacg cgtcatcggg agttccggca
     4381 ctatcataaa cctcggggag attgcagccc ggaccgcaac cggtccgaga tccgcggggc
     4441 cctggatcct ggatcgaaag ggcctctcgc gcgtgatccc gctcctctgt tccctccctc
     4501 tcaaggagag gcggaacgtg ccgggtatca accctgaacg ggccgacatc atcatcgccg
     4561 gggctgcagt cctggactgc ctgatggagg cgttctcgtt cgataggata gagatcacgg
     4621 ggagaggtct tcaggacggg ctccttgcag actacctcac caggatggaa gattttccgc
     4681 tccttggcca gctcaacgtc cgtgaacgca gtgtgctgca gctcgcccgc tcctgcgggg
     4741 tgaacgaata tcatgcacgc accgtggcca ggcttgcgct cgagctcttt gactcttcaa
     4801 aggattcagg gctccattcg ctcggggatg aagagcggga actcctcctc tatgccacgt
     4861 tcctgcacga tgtcggttct ttcatctcat accaggacca ccatgtccat tcccattacc
     4921 tcatcagcaa cagcgacatg cccggcttct cccgggaaga agtgctgatc atggcaaata
     4981 tcgcccgttt tcaccggaaa aaggcaccgg gaaaacgtga actggcagga accgggcttt
     5041 caacggcaga ccagaagagg gttcgcgcac tcacttccat gctccggatg gccgagagcc
     5101 tggatcggag ccatgcggca ctggtccgcc acgcccaatt ccggcatgta aagggagaaa
     5161 gggcgacgct tctcgtcagc gcagtggggg agtgccagct cgagatgtgg gggatcgagc
     5221 acgaagcagg ttatttccgg cgggtatttg gaaaaacgct cgcgatagag gtctgccgga
     5281 cagagttgag cgagatacgc gagcgggagg acgggctgct tccaaacctc ctcaaccatt
     5341 cgatgtcaag cggaagggag agatattcca gggagggcga cgcagggcag ggcaggaaaa
     5401 aagcaggaag gccgggccgc tgacgccgaa gcctccgatg caacccgcgc gaacatccgg
     5461 tccttcgtac aggctgtgga aaggaagccc gggcaacctc ccctctccaa ccattcatcc
     5521 cctcgcagcc cgagtaatgg ggagtgagat cacgtatcct gccgttgcga gaaggatgat
     5581 cgttgcgcct gacgggatat cgagcaggta agacagcaaa agccccccga tggaaaataa
     5641 aaggcccaaa cagaccgagg ctgccatcat ggtatggagc ctctttgtga acccgcgtgc
     5701 cgttgccgcg gggagggtga ggagtgcgat gacaaggata attcccacca cccttatcag
     5761 catgaccacg gtgactgcaa caagcaccag caggaggagg gagagccggg tcaccgggag
     5821 gttcatcacg cgggcatact cctcgtcgaa cgtgaccgcc tgcaactgcg tgtagagtat
     5881 cgctaccacg gccaggataa tggcgcagag cacagccatc aacaggatgt cttgtcccgg
     5941 caccagcagg atattgccaa agagatagct gaagaggtca ggggcataac cgggcgcaag
     6001 gctgatacag atgatcccga gtgccattcc cgatgcccag accgccgcca caagggtgtc
     6061 gaggttctgc ccggccttct ccttcaggat acctatcccg agtgcgatta ccgtggtgaa
     6121 cccaaacgct cccgcgaggg ggtccagtcc gaagagatag ccgagtccga cccccccgaa
     6181 cgccgcgtgg gagattcccc cggagatcga gaccatgcgc ctgaccacca cgtagcttcc
     6241 gatgattccg caggagatgc tggcaaagag acctgcaagg agggcgttct ggaagaaccc
     6301 gtactccagg atcccaaggt ctatcattgc ccctcctcac ccccgtgatc atcgaagacc
     6361 ctgtgcggca gtccgtgtgc gatgaggtct acagggcagt gataggtcgc agcgatcatg
     6421 tcctccgtga tctcgggggt attgtgcgta tggagcgtgt ggttgaggca ggcaaccttc
     6481 gtgacgtgct ttgagatcac cccgatatca tggctcacca ggaggattgt catggacctc
     6541 ttcagttcat ccaggatatc gaagaactgg aacgcggtgg gggtatcgac atatactgtc
     6601 ggctcgtcga ggatgaggac ccggggattc ccggccaggg cccgtgcaag gatcgcccgc
     6661 tgctgctccc ccccggagag gtgagagagc gtccttcccg ccagccccga aaggtcaagc
     6721 ctttcgagga ccttgcgaac agccgcgtgg tcctcctcgc ggtaccgcct gagcattccc
     6781 ctcgtatggg agagccggcc cgagaggacc atctctttca cggtgatggg atagcgaaaa
     6841 tcgaacgtcc ggaactgggg cacgtacccc actatctgcc tcccctctcc cggggatttc
     6901 ccatagattc tgaccgttcc ggaccgtgcg gggaggagac caaggatgat cttgaggagg
     6961 gtggtcttcc cccctccgtt tggcccgata agggcataca tgtcttgctc catgacggag
     7021 agcgagacgc tgtccaggat aggactcccg tcctgcccga acgagacgtc tcttacctct
     7081 attgccggga tcacggaaca gcacccccgt tcccctctcc atagatcaaa gatatcgcag
     7141 aagcgacccg ctcgaggttc gagagggtgt cgggggcgag ggggtcgatc agcaccaccg
     7201 agccgtggat ctcccgtgct atcacttcgc tcccccgcgt gtcatattgc ggttcggcaa
     7261 ataccacccg tattccgttc tcacgcgcaa aggtaatcaa cgacgaaagc tcccgtgcac
     7321 cgggttcctt gccctctgcg cccactgcca cctggacgag cccgtactcg cgggcaaagt
     7381 acccccacgc ctcgtggaat accaggaacg gttttccttc catgccggag agcatgtccc
     7441 tgatgttcgt gtccgtcgca tcgactctgc ggatgtacgc ctcctttcct gctgcataat
     7501 cggccgcacg gccgggatca attatcgaga atccctccgc catgtgccct gccatgatgc
     7561 gcaggttcga aggcgaaagc cagacatgcg ggtcgcctcc ctgcatggcg ccgtggtcct
     7621 ggccttgcac cctgatgagg gagactcctt ccgaggtatc gacgaccacg agatccgggt
     7681 tcagggtcaa gaggcggtcg accagcgcat cctcgaaagg cagcaacccc cggccaagcc
     7741 ggaagtacat gtccgcctgc gagagccggg cgatctggcc ggcggacggc tcgtacgtat
     7801 ggggctcgac accgggcggc acgaccaccg tgatacacac cttctcgcct ccgactgcgc
     7861 gtgcgatctc ttcctgggga aggatggtga cagccacctg cagctcggac ccgttctccg
     7921 acccgttgtc aaggcacccg gccgaataca tcgcggccag gaccagcagg agggagagcc
     7981 atagtaatcg cgctgcccct gcacccatga ccacggttcc atcccgggca ggcccgggag
     8041 ttctcccatg cgttccaccc tcacccttcg tcaacgcagc atcccctgtc atggctgatg
     8101 gcgccgcata tcccgcgggt aggatggccc agtgactggc acatcttgtt gatggcatcc
     8161 ctggagacgt atgactcgaa ccgctcggcc tccctgcacg cctcttctcc tgcgaggccg
     8221 taatgggtga ggatgaggcc aaggatacgg tggcgcctca ccaggaattc tgcatattca
     8281 tgccccttcc cggtgagcac cactcccgtg taaggccggt ggtcaacaag gcccgtggac
     8341 gcaagttccc ggaccgtctt tgagaccgtg gagggatcaa cggtaaaatg cgcggcgatc
     8401 tccccggtcc ttgcagtctc gtccctctcg tacaggtatt tcaggtatgc aaccttcctc
     8461 ggcgagagtc ccgccacatc acactgatcc attggggatc agtgggaggt gcgaggataa
     8521 aaaatgttgg agcagtacca acaatcctgc ggcagggcgc ctcgtcacat gagggaaggc
     8581 ccggagatcg tgaggtttcc catcagggtc gggacctgga accgcccgaa gaacgcctcc
     8641 tcctgggaaa gtggctggag ggggacatcc agcacgatag tctcgccctc cccgggatag
     8701 tccgcaattt tggtgtaata ctgcaggaag ccctctgctc tcacctggag gatgcgggca
     8761 ggggcagccg tgacatacac agggatgttc agcgtgccgt tctcgattgc gcccatgtat
     8821 cttccatcca ggaagacgtg cgctccctct gcactacaca tgacctggaa gaaccccacg
     8881 ctccccccga tcacctgcgg gtgcacgttc accactgtga cagcatctgc cgtggccatg
     8941 catccctcgg cgcatatcac caggcggaca gtatagactc cgggaagggg gtaaagatgg
     9001 cagggggaag gttctgttga tgtcgccccg tcgccaaagt cccatgacca ggagtcggga
     9061 tttccggtgg agttgtcctg gaactggacg tgcagcggtg ccgcgccgga ggttgtattg
     9121 gcgaggaaac cggccgtgag gggttccttt tctctgacag tgatgaattg ctccttcctg
     9181 gtgaggttgc accctcccgc accgcacacc tcgaggctca ccg
//
LOCUS       DAOQYR010000084         9200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_8019_length_9200_cov_8.624494, whole genome shotgun sequence.
ACCESSION   DAOQYR010000084 DAOQYR010000000
VERSION     DAOQYR010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 9200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9200
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8019_length_9200_cov_8.624494"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="PKM87_09275"
     CDS             complement(<1..722)
                     /locus_tag="PKM87_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNQ30296.1"
                     /translation="MIFNALIRRRIQRNPLRYQDLQSELISTRAGLTLERYLIRSMEV
                     ALLAGAGFAVLGYILTQLLVISAQSGGYIHIYNVFNLPIPGEIDPLAPEMLGIKLAGA
                     IIAFILGSLLVYLIALKYPGMQKGNRATKINLTLHNAVAYMYAMRKGGAQLMTIFRSL
                     SENSKIYGEVALEFRQVVRDADFFGYDVVSAIRHLMETTPSEKLKQFLEDMVSVIESG
                     GDLVSFLSGRVRLYQEEARFEQ"
     gene            complement(719..2383)
                     /locus_tag="PKM87_09280"
     CDS             complement(719..2383)
                     /locus_tag="PKM87_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HNQ30297.1"
                     /translation="MPLKVLDLKGIADRLKPVKGKDEIDTTPHDFSITRVISRKKSSA
                     EMPEYDFSGHGPLVDAELPPGYREVERYWVDEGRSLVDIALNENTNQYEYLLYEPSLS
                     EFEYELLERIHEDMRSALILTDEELGKDRRALLMEKMHALLHEYGITLGERSIFCLQY
                     YLVRNFIGWGRIDALMRDPYLEDISCDGHRIPIFLYHRKYRNVKTNIMFEAEVLNSLA
                     ITLAQRSGKHISTGTPMLDATLPDGSRLQLTLGTEVTTRGTSFTIRKFREDPFSPVEL
                     MEYGTFSSDELAYFWMAIENNKSLLFIGGTASGKTSSLNAVSLFIPPMAKVVSIEDTR
                     EITLFHENWIASVTRETVAEGSNIISMFDLLRAAMRQRPEYILVGEVRGVEAQTLFQA
                     MNTGHTTFSTMHAGSVDAAIHRLENEPLNVPRNMVTALNIVSVQALVYRGMERVRRCL
                     EIVEIAGIDPSTGNLRVNTVFVYDPIRDTFSYKGRSQIYADIAERRGWSRERLEEEIN
                     TRKKILDAMQKQRIIDYIRVSRIFHAYSINPSRVTENLEDLSRILE"
     gene            2587..3411
                     /locus_tag="PKM87_09285"
     CDS             2587..3411
                     /locus_tag="PKM87_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HNQ30298.1"
                     /translation="MHDTARRTMPTGISSLDAVMEGGMPPGSLILLLGDIGGGNNEFV
                     YSSIMLLSVMKQKPAGEDFLLPEEIDYITFTKGRSDILEEMKRSFNPDLTQALGDVKF
                     KDLSQIYFDSSIIPLEWYSDKDIISRLQSRGGHENILAYLAAQLNTVPKNSLVVLDSI
                     TDLATQFNKIGKWNDFSAFLRGLQRVAKEWNTTVYLLLTKGILDAPRELEVADASDAV
                     LFFKWEETTGARRQRVMYIEKFRGLMPHLEEKDLVKFAVRITSAEGFDVSNIRVVI"
     gene            3408..4106
                     /locus_tag="PKM87_09290"
     CDS             3408..4106
                     /locus_tag="PKM87_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KaiC domain-containing protein"
                     /protein_id="HNQ30299.1"
                     /translation="MSEGRVKIGIEGLDQMMGGGLIPGSICSIIGTYGTGKTTFALEF
                     IWEGLKKGENVIYISLEEREDRILRYMEEKGWDIAPYREHALSVLKLDPSDFNLAINS
                     IKNELPALVKQVGAQRVVIDPVSLFEDLFDRDSDRRREMFRFVDTLRDQHCTIVMTSE
                     TDKDNPFASRHNLIEYLADTVILLRYVRPSDLTDVHLALEVVKMRLSAHSREIKPYEI
                     QQGQVLVYTEANVF"
     gene            complement(4327..4593)
                     /locus_tag="PKM87_09295"
     CDS             complement(4327..4593)
                     /locus_tag="PKM87_09295"
                     /inference="COORDINATES: protein motif:HMM:NF019085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HNQ30300.1"
                     /translation="MNLDFAVLIAGIIIAVALFFLIKDITKLIINSILGLLILFFANL
                     FNIMGSLGRTDIPYSLVNVLLCILGGIPGALIVIVLHLLGIEGF"
     gene            complement(4596..5879)
                     /locus_tag="PKM87_09300"
     CDS             complement(4596..5879)
                     /locus_tag="PKM87_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNQ30301.1"
                     /translation="MELIDRILKEGEKRVDELEVYFFSGSAVTVRLKKKEIHYASGSD
                     HQGLSLRVINEGRIGASSTSNPERWEGCLDAAIASARLATPQDWKGLPGPRDLDFTPL
                     NFDPAVIPDADTAGTLVQGLLEGAAAYPVEITSGSAEVARSHAVLANSSGIRYHENES
                     MVSVGVETIVGQSTGYEFDVSCRMDIDPQKVGERAAYLAYSSQGGKDIGSGHYDVVLS
                     PVALSQFLGSVFVPALSGKNVHAGRSKLAGLLDQEIMDGDLILLDDPSHPKGLSSCRW
                     DAEGIPTRKVNFVDGGVLREFAYDLKTAYRYGMESTGSAVRGGHGGSPSIGVHTLTLD
                     GLRNDLLDGAVVHVHDVVGAHTANPLSGDFSVELSNALFMRDGNVEFPVRKAMLSGNV
                     FEMLKETGGLSQDERVVGSMVLPEIRLKNLAIIGN"
     gene            complement(5866..7197)
                     /locus_tag="PKM87_09305"
     CDS             complement(5866..7197)
                     /locus_tag="PKM87_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNQ30302.1"
                     /translation="MARARYFDVRHVTGTVTHVDIDNTRVESAGTSFLDRAVLRVLGP
                     RGWGILAIDHYTRPKGRRFDELVERGAGLASLTGQEVLLADFPSESIRVPPMKENPAT
                     VELDEKQRLLADIERSARLEGIKSTRANYIERFEEVQFEDSSGNEYSCAVCRSGFSIL
                     AVADRSGDLQMGYVRKHTLQDFNLRHRQDDGLMAAERAIALLDARPPRGGTMNAVLDP
                     ELAGVFAHEAVGHASEADLVQEGSSVLAGRIGERIGSPAVTIVDDPSLPEFGFEPFDA
                     EGCRVGRTEIIRNGVMNAFLHSRETLAAVGHGNAGNARAMPGEPPQVRMSNTFIENGD
                     SSYDEIVQECNSGILLKGTRGGQVDPGRGVFQFNAEYGYLIKDGEIGEMVRDVSLSGE
                     ILMTLHSISLCAGDRIMHQGYCGKGGQTVPVSDGAPHVLLRDAVVGGSGTD"
     gene            complement(7202..9118)
                     /gene="lonB"
                     /locus_tag="PKM87_09310"
     CDS             complement(7202..9118)
                     /gene="lonB"
                     /locus_tag="PKM87_09310"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HNQ30303.1"
                     /translation="MADEQVVEGNQEEEAPVEVSTLNSAQVDVPPSLIDQVIGQEHAV
                     EVIRKAAIQRRHVMMIGSPGTGKSMLAKAMAELLPKEEMQDILVYPNSDDSNNPIVRT
                     VPAGRGKQIVAAHKAEARKKTQLRNTLFLLLLVGIIGYAFITYQWLMGIIAAAFIFMA
                     LRYATPREESMVPKLLVSNDATTTAPFIDATGSHAGALLGDVRHDPFQSGGLETPSHD
                     RVEAGAIHRSHKGVLFIDEINTLTPHSQQNLLTALQEGEFPITGQSERSSGAMVRTEP
                     VPCKFIMIAAGNLDAIQGMHPALRSRIRGYGYEVYMSESMLDTPENREKFIRFIAQEV
                     KNDGKIPHFDKGAMEEIIREARRRSNRKGHLTLKLRDMGGLIRVAGDLARQQGAEVTT
                     SDHVIAAKKTARSIEDQVSDEYIHRSREYELTVVEGTRVGRVNGLAVMGSDSGSVLPI
                     MAEVTPAQGASGTVIATGMLKEIAQESIKNVSAILKKFTGKDIKNMDVHIQFIGTYGG
                     VEGDSASISVATAVISAIESIPVRQDIGMTGSLSVRGDVLPVGGVTYKIEAAAKAGIK
                     TVLIPRANIEDVLIEERYQSMVTIIPVDHIEDVLKIALVPENREGFLNKLRKMASTPS
                     GSFLDTTGIPTSPA"
BASE COUNT         1814 a         2706 c         2660 g         2020 t
ORIGIN      
        1 ttctgctcaa accttgcctc ttcctggtag agcctgacac gtcctgacag gaagctcacg
       61 aggtccccgc cgctctcgat caccgagacc atgtcctcca ggaactgttt cagcttctca
      121 gagggagtgg tttccatcag gtggcggatg gcggatacca cgtcgtaccc gaagaagtcc
      181 gcatcccgca cgacctgccg gaactcgagg gccacctccc cgtaaatctt tgagttctcg
      241 gagagggagc ggaaaattgt catcaactgc gccccacctt tccgcatggc atacatatag
      301 gccaccgcgt tgtggagggt gaggttgatc ttggttgccc ggtttccctt ctgcatgccg
      361 ggatacttaa gtgcgataag atacacgagg agcgagccaa gaataaaggc aataatcgca
      421 ccggcgagtt ttatgcctag catctcgggt gcgaggggat caatctcacc gggaatgggc
      481 aggttgaata cgttgtagat gtggatatag ccacccgatt gtgcggagat tacaagtaac
      541 tgtgtcagga tgtacccgag gaccgcaaat cctgcgcctg ccaggagggc aacttccatc
      601 gaccttataa ggtaacgctc gagcgtcagt ccagcccggg tggagatgag ttcggactgg
      661 agatcctggt aacgcagggg attgcgctgg attctcctcc tgatcagggc gttgaagatc
      721 attccagtat cctggagagg tcctcgaggt tctctgtcac gcgggagggg ttgatgctgt
      781 aggcatggaa gatcctggat accctgatgt agtcgataat cctctgtttc tgcatggcgt
      841 cgagaatttt cttgcgggtg ttgatctcct cttcgagcct ctccctgctc caccccctcc
      901 gctcggcgat gtctgcataa atctgcgatc tgcccttgta gctgaatgta tcacggattg
      961 gatcatacac gaagacggta ttgaccctga ggtttccggt cgaaggatct atcccggcga
     1021 tctccacgat ctcaaggcac ctgcgcaccc tctccatgcc gcggtatacc agggcctgga
     1081 cactgacaat gttgagggcg gtcaccatgt tcctcgggac gttgagtggc tcgttctcga
     1141 ggcggtggat ggccgcatcc acgctccctg catgcatggt cgagaatgtg gtgtgcccgg
     1201 tgttcatcgc ctggaagagg gtctgtgctt caacgcccct gacctcgccg accaggatat
     1261 attcgggtct ctggcgcatg gcagccctga ggagatcgaa catcgagatg atgtttgacc
     1321 cctctgcaac cgtctccctc gtcacgctcg cgatccagtt ctcgtggaag agggtgatct
     1381 cgcgggtatc ctcgatgctc acgaccttgg ccattggggg gatgaagagg gagacggcgt
     1441 tcagcgaaga ggtctttccc gacgcggtcc ccccgatgaa gaggagactc ttgttgttct
     1501 cgatggccat ccagaagtat gcgagctcgt ccgaactgaa cgtgccgtat tccatcagtt
     1561 ccacgggaga gaatggatcc tcgcggaact tgcgtatggt aaacgaggtt cccctcgtgg
     1621 tcacctccgt cccgagtgtc agctggagcc ttgagccgtc aggaagggtt gcatcgagca
     1681 tgggggtccc ggtcgagatg tgttttccgg acctctgggc gagggtgatg gcgagggagt
     1741 tgagtacttc cgcctcgaac atgatgtttg ttttgacatt ccggtacttc cggtggtaga
     1801 ggaatatggg gatgcggtgc ccgtcgcagg agatatcctc gaggtacggg tctctcatga
     1861 gggcatcgat cctcccccac ccgatgaagt tccggacaag gtaatactgg aggcaaaaga
     1921 tgctcctttc cccgagcgtg atgccgtatt cgtgcaggag tgcgtgcatc ttctccatca
     1981 gcagcgcccg ccggtctttg ccgagctctt cgtcggtaag gatgagggcg gaacgcatgt
     2041 cctcgtggat ccgctcgagg agttcgtatt cgaactcaga gaggctcggc tcgtagagga
     2101 ggtattcgta ctggttggtg ttctcgttca gtgcgatgtc gacgagggac ctgccctcgt
     2161 ccacccagta gcgctcgact tcacggtatc ctggcggcag ttccgcatct accaggggcc
     2221 catggcctga aaagtcatac tccggcattt ccgcggagga ttttttacgg gatattacac
     2281 gcgtaatgga aaaatcgtgc ggcgtggtgt caatctcgtc cttgcccttc accggcttca
     2341 gccggtcggc aattcccttc aggtccaaaa ctttcagcgg cacggaattc cccactccct
     2401 gcgaccaggc cctgggcgcg ttctcttctg taggattggt atccctgcac acctatataa
     2461 aagcatgatt gggggaccat tgcaatgagg tcttcaaccg cgtctggagc cattttcttg
     2521 cccgtctgac agaaatctac atgtatgggc ggaccctcac tcttcatgga ataacagagg
     2581 ttctgcatgc acgatacggc gcgccggaca atgcccaccg gaatttcatc actcgacgct
     2641 gtgatggagg ggggaatgcc ccccgggtcg ctgatcctcc ttcttgggga tatcggggga
     2701 gggaacaacg aatttgtcta cagttccatc atgctcctct ccgtgatgaa gcaaaagccg
     2761 gcaggggagg acttcctgct tcccgaggag atcgattata tcacgtttac caaaggcaga
     2821 tcagacatcc tggaagagat gaagcggtcc ttcaaccccg atttgaccca ggcactcgga
     2881 gatgtgaaat tcaaggatct ctcccagatc tactttgatt cgagtatcat tcccctggaa
     2941 tggtattcgg acaaggacat catatccagg ctccagtcaa ggggggggca cgaaaatatt
     3001 cttgcgtatc tggcagcgca gctgaacacg gtgccaaaga acagcctggt agtgctcgac
     3061 tccatcaccg acctggcaac ccagttcaac aagattggca agtggaatga tttttcggcg
     3121 ttcctacgcg ggttgcagcg ggtggcaaag gagtggaaca ctaccgtata ccttctcctc
     3181 acgaagggga tcctggatgc cccgcgggaa cttgaggtgg cagatgcgtc ggatgccgtc
     3241 ctcttcttca agtgggagga gaccacgggg gcaaggcggc agcgagtgat gtacatcgag
     3301 aagttccgcg ggttgatgcc ccatctcgag gagaaggacc tggtgaagtt tgccgtccgg
     3361 atcacctctg ccgaggggtt cgatgtcagc aacatccggg tggtcatatg agcgagggtc
     3421 gggtgaagat cgggatcgag ggccttgacc agatgatggg cgggggcctc atcccgggga
     3481 gcatctgttc catcatcggc acgtatggaa cggggaagac cacctttgcc ctggagttca
     3541 tatgggaagg cctcaagaaa ggggagaatg tcatctacat cagcctcgaa gaacgggagg
     3601 accggatcct gcgctacatg gaggagaaag ggtgggatat cgccccctac agggagcatg
     3661 ccctctcggt gctgaaactc gacccctccg acttcaacct ggccatcaac agcatcaaga
     3721 acgaactccc tgccctcgtg aagcaggtcg gcgcgcagcg ggtggtaatc gacccggtct
     3781 cactctttga ggatctcttc gaccgtgact ccgacaggag aagggagatg ttccggttcg
     3841 tagacaccct tcgggaccag cactgcacta tcgtgatgac gagcgagacc gacaaggaca
     3901 atcccttcgc cagccgccac aacctcatcg agtacctcgc cgacacggtg atcctcctgc
     3961 gctacgtccg cccctcggat ctgaccgacg tgcaccttgc gctcgaagtg gtcaagatgc
     4021 gcctctccgc ccattcacgg gagatcaagc cgtacgagat ccagcagggc caggtgctgg
     4081 tgtatacgga agcgaacgtg ttttgagcag ttggggctta aaaagcccca actgcggggg
     4141 tatgcgcagg aacaggcctg aaggagtcaa tcgattgtct gatgcgggaa ttggcttaaa
     4201 aagccccaac tgagggggga ttggacggga acaggcccaa aggagtcaat cgattaacct
     4261 atacagggat atctaaaaag ctcccttttc gcagatgaga gggagcagga tctactccct
     4321 cttattctag aacccctcga tccccagcag gtggagcacg atcacgataa gggctcccgg
     4381 gatgcccccc aggatgcaaa ggaggacgtt cacgagggaa taggggatgt ccgtcctccc
     4441 gaggctgccc atgatgttga agagatttgc aaagaagagg atcaggaggc caaggatcga
     4501 gttgatgatc agcttcgtga tgtccttgat gagaaagaag agcgccacag cgatgatgat
     4561 tccggcaatc aggactgcga aatctaggtt catgcctaat taccaatgat cgccaggttt
     4621 tttaatctta tctcaggcag gaccatcgat cccaccaccc tctcgtcctg cgagagcccg
     4681 ccggtctcct tgagcatttc aaatacgttc cctgagagca tggctttcct gaccgggaat
     4741 tcaacattcc catcccgcat gaagagggca ttcgagagtt ccacggagaa gtcaccggag
     4801 agcgggtttg cggtgtgggc acccaccacg tcatgtacgt ggaccaccgc gccatcaagg
     4861 agatcgttcc tcagcccgtc gagggtgaga gtgtggacgc cgatcgaggg ggaccccccg
     4921 tgccctcccc tgaccgcact tccggtgctc tccatcccgt accggtacgc ggtcttcagg
     4981 tcataggcaa actcgcggag gactccccca tccacgaagt tcacctttct cgtgggaatc
     5041 ccctccgcat cccacctgca gctcgagagg ccttttgggt gggacgggtc gtcgaggaga
     5101 atgagatcgc catccatgat ctcctggtca aggaggcctg caagttttga acggccggca
     5161 tggacgttct ttccgctcaa cgccgggacg aacacgcttc caaggaactg tgagagggcg
     5221 accggggata agaccacgtc gtagtgcccg ctgccgatat cctttcctcc ctgcgaggag
     5281 taggccaggt atgcagcccg ctcacccacc ttctgcggat cgatatccat cctgcaggac
     5341 acgtcgaact cgtatcccgt ggactggccg acaatcgtct ccacacccac gctcaccatg
     5401 ctctcatttt cgtggtatcg gataccgctg ctgttggcca gcactgcgtg cgaccttgcg
     5461 acctcggcgc ttcccgatgt gatctctacc gggtaggcgg ccgcaccttc gaggagcccc
     5521 tgcaccagcg tgccggcggt gtctgcatcg gggatgaccg cagggtcgaa gttcagcggg
     5581 gtgaaatcga gatcgcgggg accgggcagc cctttccaat cctgcggagt cgcgaggcgg
     5641 gcgctggcta tcgcggcatc aaggcagccc tcccatcgtt cgggattgct ggtcgaagat
     5701 gcgccgattc tgccttcgtt gataacccgg agagagagtc cctggtggtc cgatccggaa
     5761 gcatagtgaa tctccttttt tttgagccgg acggtgactg cggacccaga gaaaaagtag
     5821 acctccagtt catcgactct cttctcgcct tcttttaata tacggtcaat cagttccact
     5881 gccgcccacc accgcgtccc gcaggagcac gtgcggtgcc ccgtcgctga ccggcaccgt
     5941 ctgcccgccc ttgccgcaat atccctggtg catgatgcgg tctcctgcac agagtgagat
     6001 cgagtgcagg gtcatgagga tctcgcctga aagggagaca tccctcacca tctccccgat
     6061 ctcgccgtcc tttatcaggt atccatattc cgcattgaac tggaagacgc ccctcccggg
     6121 atccacctga cctcccctgg ttcccttgag caggataccg gaattgcact cctggacaat
     6181 ctcatcatac gagctgtccc cgttctcgat gaaggtgttg ctcatccgga cctggggagg
     6241 ttcgccgggc atggcccgcg cgttcccggc gttgccgtgc cccaccgcgg caagcgtctc
     6301 ccggctgtgg agaaatgcat tcatcacccc gttccttatg atctcagtcc tccccaccct
     6361 gcacccttca gcgtcgaagg gctcgaaccc gaactcgggg agcgaggggt catccactat
     6421 ggtgactgcc gggctcccta tacgctctcc tattctccct gcgagcaccg agctgccctc
     6481 ctgcacgagg tcagcctcgc ttgcgtggcc caccgcctcg tgtgcaaata ctccggcaag
     6541 ctcggggtcc agcaccgcat tcatcgtgcc cccccgcggc ggacgggcat cgagaagtgc
     6601 aattgccctt tctgcagcca tcaggccgtc atcctggcga tggcgcaggt tgaagtcctg
     6661 caaggtgtgc ttccgcacgt accccatctg caggtccccc gaccggtccg cgacggcgag
     6721 gatggagaag cccgacctgc agacggcgca ggagtattcg ttcccgctgc tgtcctcgaa
     6781 ttggacctcc tcgaaccgct cgatgtaatt ggccctggta cttttgatcc cctcgaggcg
     6841 tgcacttcgc tcgatatcag ccaggagcct ctgtttctca tcaagctcaa cggtggcagg
     6901 gttctctttc atgggcggga ctctgatact ttcggaggga aaatcggcga gcagcacctc
     6961 ctgtcccgtg agagatgcaa ggcctgcgcc cctctcaaca agttcatcga accgcctccc
     7021 tttcggccgg gtgtaatgat cgattgcaag gatgccccat ccccgggggc cgagcacccg
     7081 gagaacggcc ctgtccagga aggacgtgcc tgccgactcc acgcgggtgt tgtcgatgtc
     7141 cacgtgggtg acggtgcccg tcacgtgccg gacatcaaaa tagcgggcac gtgccatggc
     7201 ttcacgccgg ggaggttggg atccctgtcg tgtccaggaa agatcctgaa ggggtcgatg
     7261 ccatcttccg cagcttgttc aggaaccctt cgcggttctc gggcaccagc gcgatcttca
     7321 ggacgtcctc gatgtggtcg accgggatga tggtgaccat tgactggtac cgttcctcga
     7381 tcaggacatc ctcgatattt gcccggggga tgagcacagt cttgatgccg gcctttgcag
     7441 ccgcctctat tttgtaggtc actcctccta caggaagcac gtcaccgcgg accgaaaggg
     7501 atccggtcat gccgatgtcc tgtcgcacgg gaatgctctc tattgcgctg ataaccgcgg
     7561 tcgcaacgct gatggatgcg gaatcacctt caacgccgcc gtaggtcccg atgaactgga
     7621 tatggacatc catattcttg atgtccttcc cggtgaactt cttcaggatc gcgctcacgt
     7681 tcttgatgga ctcctgggcg atctccttca gcatccctgt tgcaatcacc gtgccgctgg
     7741 ctccctgagc cggcgtgacc tctgccatga tggggagcac cgagcccgaa tcactcccca
     7801 tcaccgcgag gccgttcacg cggccgactc ttgtcccttc gaccacggta agctcgtatt
     7861 ccctgctccg gtggatatac tcgtccgaga cctggtcctc tatggacctt gcagtcttct
     7921 tggcagcgat tacgtggtcg gaagtcgtca cctcggcacc ctgctgcctc gccaggtccc
     7981 ctgccacgcg gataaggccg cccatgtccc ttaacttgag agtgaggtga cccttccggt
     8041 tggaacgcct gcgggcctcc ctgataatct cctccattgc gcccttgtcg aaatggggga
     8101 tcttgccgtc gttcttgact tcctgtgcaa tgaaccggat gaacttctcc cggttctcgg
     8161 gcgtgtccag catgctctcg ctcatgtaga cttcgtatcc atacccgcgg atacgcgagc
     8221 ggagcgcagg atgcatcccc tggatagcgt caagatttcc tgccgcgatc atgatgaact
     8281 tgcatggcac gggctcggtc cggaccatgg ccccgctgga gcgctcgctc tgcccggtga
     8341 tggggaactc cccctcctgg agggcggtga ggaggttttg ctgggaatgg ggtgtgaggg
     8401 tattgatctc gtcgatgaag agcacccctt tatgagacct gtggattgcg ccggcctcga
     8461 cccgatcgtg gctcggggtc tccaggccgc cgctctggaa cggatcgtgc ctcacatccc
     8521 cgagaagcgc tcccgcgtgc gaacccgtag catcgatgaa gggtgcggtg gtggtggcat
     8581 cgttggagac gaggagcttc ggcaccatcg attcttccct tggcgtggca tagcggagcg
     8641 ccatgaagat gaacgctgca gcgatgatcc ccatcagcca ctggtacgtg atgaaggcat
     8701 acccgatgat cccgacgagg aggagcaaaa agagggtgtt tctcagctgg gtcttcttcc
     8761 ttgcctccgc cttgtgcgca gccactatct gttttccgcg cccggcaggc acagtcctca
     8821 cgatggggtt gttcgagtcg tccgagtttg gatacacgag gatgtcctgc atctcctctt
     8881 ttgggagaag ctcggccatg gcctttgcga gcatcgactt ccccgtgccg gggctcccga
     8941 tcatcatgac gtggcgcctc tggatggcag ccttcctgat gacctcgacc gcgtgctcct
     9001 ggccgatgac ctggtcgatg agagacggag ggacgtcgac ctgtgcggag ttcagcgtgg
     9061 agacctcaac cggcgcctcc tcctcctggt tcccttcaac aacctgctcg tcagccatgc
     9121 gaaaattgac ctctcctgtc actaaagata gtgatataat ttattcctgc tcgtttaagt
     9181 actttcagcc tgaggtagcg
//
LOCUS       DAOQYR010000085         9057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_8180_length_9057_cov_8.371917, whole genome shotgun sequence.
ACCESSION   DAOQYR010000085 DAOQYR010000000
VERSION     DAOQYR010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 9057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9057
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8180_length_9057_cov_8.371917"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1438
                     /locus_tag="PKM87_09315"
     CDS             176..1438
                     /locus_tag="PKM87_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ30304.1"
                     /translation="MTLRDAILLQKRELELRMQEKYVPRDVNLPVEENRLIKVIIGPR
                     RAGKSFFAVHSLMDKGQFGYVNFDDEQMTGSQVLSEIGPAIESVYGTVSTLLLDEVQN
                     LFEWELFVNRLSRQGYNLYITGSNAHLLSKDLATHLTGRHTLTTILPFSYREYIRLKG
                     EELTESEYREHLDLYSETGGYPEPLVTNINRKEYLSRLFDAIIFKDIIRRYNVRAPQG
                     LGDLATYLCSNSASEYSVHRLTKVTGCKSDLTVKKYLHYLEEAFLFFSIPRFSFKVKE
                     QLSSHKKIYCIDNGFITAKGFRVSGDKGKLYENLVAIALKYQEFLDNLRVFYWKNSQA
                     EEVDFVIQRERQVSELIQVCVDAQSPAARQREMKALVKAGRDLKCDRLLLLTREEEGE
                     EEIEWFGMKGVVRIMPLWKWLLDSTCLK"
     gene            2018..2743
                     /locus_tag="PKM87_09320"
     CDS             2018..2743
                     /locus_tag="PKM87_09320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ30305.1"
                     /translation="MTGTNRSARTRAGPKLPGASLHPLSLSVVPLVKADAEAAAELYT
                     RVFLDDEPTTRCHGLAPARFLPFARCYVRWLVEKDLSFVAKDERTGEALGFIFCFDFR
                     DDPAQDSPVLREFLQQFRHAIAMIDELEARHICRDEIEPGSILHIFQIGVDRKGRRQG
                     IARGLINRVLSHANERGFLQMVADCTNPASRRLFEACGFSEVGISSYESFTIDGRCIF
                     DGLEGGISLMIRDVSPRTLLPFS"
     gene            2848..5394
                     /locus_tag="PKM87_09325"
     CDS             2848..5394
                     /locus_tag="PKM87_09325"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNQ30306.1"
                     /translation="MPPFDLSCYPGLSAKDARRRLLDEGPNELPSREARGTIGIIKDV
                     FREPMFLLLVGAGAIYFVLGDIEEGALLLSFVLVIIGITVYQEQKTERALEALRNLSS
                     PRALVIRDGQLARIAGRDVVRGDFLLVSEGDRIAADALVHASTNLSVDESLLTGESVP
                     VRKLPAEKDPGERQPGGDDQPWLYSGTLVVQGQGIAEVVSTGIHTRMGEIGKALQAVK
                     QEKTRLHIETARIVRSIAVIGLFLFILVVVAYGLTRGDWLQGLLAGITLAMAILPEEF
                     PVVLTVFLALGAWRLSKINVLARHVPALEALGATTVLCTDKTGTLTLNRMTVAKCVAG
                     SSTYDCLAGEGSPLPEPFHELLEYAILSCKKDPFDPMEQALLRTGNRDLSGTEHLHRD
                     WELLLEYPLSRELLAMSNVWRSRKGDEYIIAAKGAPEAIFDLCHLGEEETEGMTARVE
                     DLASQGLRVLGIAKAHFRIGQLPEGQHAFHFTYLGLIGFTDPVRPDASDAVAECRTAG
                     IRVAMITGDYPATARNIARQIGLPDCDRVLTGADLDSMPPDKRDEEIHSSSLFARVVP
                     EQKLQIVESLKARGEIVAMTGDGVNDAPALKAAHIGIAMGGRGTDVAREASSLVLLDD
                     NFASIVAGIRTGRRIFANLRKAMAYIISVHVPIAGLSLIPVLSGWPLILLPVQIVFLE
                     LIIDPACSVVFEAEQAEPGIMQRPPRDPGDHVLDRPTVAIALAQGLVALCIVIGVTLY
                     GMKQGLVADQIRTISFVTIVIANLGLICTNRSWSESIIATFRKPNRAFWWVMGGTAFF
                     LAAVLLIPGLRALFRFAPVSPWYLLVCCMGGLMTVAWFEIYKVTRARGFA"
     gene            5618..6151
                     /locus_tag="PKM87_09330"
     CDS             5618..6151
                     /locus_tag="PKM87_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNQ30307.1"
                     /translation="MNQEPPLTRIHSLCSQLARHPAFLGALIGILAAFSQALLISAGG
                     PEAYGFCVACHTRDLVNGLTNIIAGTNLALAAISKNSLLPVMSVAGVLIGGYISATLH
                     REHRIKKGTIKDYFIYFFAGILVLNLAMIFGGCPYRAALRTGYGDFTAVLFIVAMAAG
                     VVLGAYLLLKKAEREDA"
     gene            6151..6675
                     /locus_tag="PKM87_09335"
     CDS             6151..6675
                     /locus_tag="PKM87_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNQ30308.1"
                     /translation="MATGILPVDAAVFFVPLVTLTLGLLIGYLAQRSGYCSIGGFRDY
                     FLFRHTRLLSGYLALIVASFAGYFLFWLVVPGAMEHFFWATTRGPFVPVPGAPGGLVP
                     TAYLIISIVTGFLVGLIAVLLGGCPIRLTVMASEGNYRAAWFFVGMCAGSIIFATIVS
                     GWVVWFINALGFGA"
     gene            6682..7845
                     /locus_tag="PKM87_09340"
     CDS             6682..7845
                     /locus_tag="PKM87_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30309.1"
                     /translation="MVSSTFLFCDLVPGERLRWIAETLRASKGTGGVPLSMTAFLTGD
                     ALYSLVDARTRDSWRTLADRDGVRVIADGDELGLHGLRDLVASGSPWVTVAGSQDEAP
                     FWQSLVSSLVSEWKGTQKAGFLLCDGPYMSRVTVYMVRFLSAVQAGGFSPELYTYLDG
                     VHALHNGQRPSEFENIGRAIAGISASSVQAGRDPWFAACSRCATARGYYQMNPGTGFC
                     EPASAIEEIAIRPLKEILSRFSGNLPVVSSASGDLVPDGWGGDRVPRLLVFIAHPPYC
                     AEWTFGGLSLALAAAMGGIPATVIFIEDGVYALHGNHEVPAHDKVFNVQEMIAVTTDV
                     PDLEYFVHGPSLDDRGIDLLPGFPTIPRLRNEDLARVFLKSESDGTASRLIFF"
     gene            7863..8078
                     /locus_tag="PKM87_09345"
     CDS             7863..8078
                     /locus_tag="PKM87_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30310.1"
                     /translation="MTEPMDFTELTCTNLMIKLKILLNKLPQGDRVAFFATREQVDNT
                     CSPFSGQGYQVSWDQAAENRYLVRLGK"
     gene            complement(8332..8646)
                     /locus_tag="PKM87_09350"
     CDS             complement(8332..8646)
                     /locus_tag="PKM87_09350"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNQ30311.1"
                     /translation="MESAHPIKLGPLKDIPVGDTKVIKIDNREILLVHLESGIYALAN
                     SCAHGACRLGYGRLEGETLRCLCHGSVFNVRTGEVLNGPATTPQPTFRVLVEDGEITL
                     VP"
     gene            complement(8871..>9057)
                     /locus_tag="PKM87_09355"
     CDS             complement(8871..>9057)
                     /locus_tag="PKM87_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30312.1"
                     /translation="DAAGNVYVTEWAANRTQKFSPSMTPAPTPTTVTRTPTAPLACAG
                     LAGLAIAMLLRARKGLE"
BASE COUNT         1866 a         2593 c         2554 g         2044 t
ORIGIN      
        1 cttaagatcc ggtcacgaag gtgttcgtgg gttcgaatcc catccctcgc attctgtttg
       61 atgacttgct aaaaatttcc taaaaaaata atttacattc cttttagtgt aatacaaaat
      121 tttatctatt tttttgtatt acaatatatt atactacaat aattcattga gaattatgac
      181 tcttcgagat gctatcctct tgcagaaaag ggaacttgaa ctacggatgc aggaaaagta
      241 tgtcccacgt gacgtgaatt taccagtgga agaaaacaga ctcataaagg tgattatagg
      301 tccaagacgg gcaggaaaat cattttttgc cgtccattct ctgatggaca aagggcaatt
      361 tggctacgtg aacttcgacg atgaacagat gactgggagt caggtattat cagaaatcgg
      421 tccggcaatt gaatcagtat atggcaccgt atccaccctc cttcttgatg aggtgcagaa
      481 tctctttgaa tgggaattat ttgtgaatcg attatccagg caggggtaca atctctacat
      541 taccggaagc aacgcccatt tgcttagtaa agatcttgca acgcacctta ccggtcgaca
      601 cacattaaca accatactgc cgttttctta tcgagagtat atccggttga aaggggagga
      661 actgaccgag agcgaatatc gggaacatct ggatctttat tcagaaaccg gtggttatcc
      721 cgagccattg gtcacgaata tcaaccggaa agaatatctt tcaaggcttt ttgatgcaat
      781 tatattcaag gatattatca ggagatataa cgtgagagca ccacaggggc ttggggatct
      841 tgcgacctac ctctgttcaa acagtgcgtc agaatattct gttcatcgat tgaccaaagt
      901 aaccggttgt aaaagcgatc tgacggtgaa aaaatatctt cattacctgg aagaagcgtt
      961 cctgtttttt tctataccac gtttctcttt caaagtaaaa gagcagttat catcacacaa
     1021 gaaaatctat tgtattgaca acggtttcat cactgcaaag ggattcagag tatctggtga
     1081 taaggggaaa ctctacgaga atcttgttgc aattgcatta aagtatcagg aatttttgga
     1141 taacctgagg gtattttact ggaaaaattc tcaagcggaa gaagtcgatt ttgttatcca
     1201 acgtgagcgg caggtgtcgg aactaattca ggtatgtgtc gatgcacaga gccctgctgc
     1261 acggcagcga gagatgaaag ccctggtgaa ggcaggacgc gatctgaaat gtgacagact
     1321 tcttctcctt accagagaag aggaaggaga agaggaaatt gagtggtttg gcatgaaagg
     1381 tgttgttcga atcatgccac tctggaaatg gctcttggat tcgacgtgtt tgaagtgaaa
     1441 gagtcacagt acagatgggg ggaaaaggaa agccccccgc ttcccaattc agccaggcct
     1501 gtccccatcg ccttcttgtt gaaccaggat ctcaaaatat ccagccgaac aatcctctgt
     1561 cagtctgctc cggctgcccg aaaatgagga gccggtcctg ctccttgcca aggacctgcg
     1621 cctcccgcat cacccttacc tgcggtgcgg gtcgcagtct gaacagttca tgagcacctg
     1681 ctccatcagc tggacgcgct gctggttcga gtcagcgtgc tggattatct taccggcggt
     1741 ttccgggggc tttcggtgcc ccgcattgca tcagcgcccg gcagtgacga taccttgata
     1801 ccgatccgga taccggggaa ttcacataag aggttgcaat gagtgaaggc caacactccg
     1861 atgtcctgaa gaaactcgtc cttgtgtcat tgctcctgcc atatccggca ccatcattgc
     1921 actgctgtgg cattatgggg attaggcacc ggcgatgtcg gtgccggcaa atctccccgc
     1981 aaccaaataa taccattgag gaatatctct ctcagaaatg acgggaacca accgatctgc
     2041 ccgaacccgt gctggcccga aactgccggg ggcctctctg catcccctat ctctctctgt
     2101 tgttcccctt gtcaaagcag atgcggaagc ggccgcggaa ctctacacac gggtcttcct
     2161 ggacgatgaa ccgaccaccc gctgccatgg cctcgccccg gcccgcttcc tcccgtttgc
     2221 ccgatgctat gtccgctggc tggtggagaa ggacctcagt tttgttgcaa aggatgaacg
     2281 gaccggggag gccctcgggt tcatcttctg ctttgacttc cgggacgacc ctgcgcagga
     2341 tagcccggtg ctccgggagt tcctccaaca gttccggcat gcgatagcga tgatcgatga
     2401 gctggaggcc cgccatatct gccgtgatga gatcgagccg ggatcgatac tgcacatctt
     2461 ccagattggg gttgaccgga aaggacgccg gcagggaatt gcccgggggt tgatcaaccg
     2521 ggtactttct catgcgaatg agcgcgggtt tttgcagatg gttgctgact gcaccaaccc
     2581 ggcatcccgg cggctctttg aagcgtgcgg gttctctgaa gttggcatct catcctacga
     2641 gtcgttcacc atagacggtc gctgcatctt tgatggcctc gagggaggaa tctccctcat
     2701 gattcgggat gtctccccgc ggacactttt acccttctcc tgatctacct ctcttcagtt
     2761 gcatgtgctg acgggaagcc cggaagcccg ccatccgcaa aacctttagg tcaggccagc
     2821 atttccctga gttaccgaat acacgccatg cctcccttcg atctctcctg ctaccctgga
     2881 ctctctgcaa aagacgctcg gcgacgtctt cttgacgagg ggccaaatga actcccctcc
     2941 cgcgaagccc gcgggacaat cggcatcatc aaagacgtct tccgggaacc gatgttcctc
     3001 ctccttgtgg gggccggggc catctatttt gtcctcggcg atatcgagga gggagccctc
     3061 ctcctgtcgt ttgtcctggt gatcatcggg atcacggtct accaggaaca gaagaccgag
     3121 cgggccctcg aagcgttgag aaacctctcg agcccgcggg cgctggtcat ccgggacggc
     3181 cagctggcaa ggatcgccgg acgggacgtc gtgcggggcg actttctcct tgtcagcgaa
     3241 ggggaccgaa tcgccgccga tgccctggtc cacgcctcca cgaacctctc ggtcgatgaa
     3301 tcgctgctga ccggcgagtc ggtcccggtc aggaaactcc cggcagaaaa agacccgggc
     3361 gaacggcagc ctggcggtga cgaccagccc tggctctact cggggaccct ggtggtccag
     3421 ggccagggga ttgccgaggt ggtctcgacc gggatccata caaggatggg ggagattgga
     3481 aaggcgctgc aggctgtcaa gcaggagaag acccgcctcc atatcgagac agcccgcatt
     3541 gtccgtagca ttgcagtcat cgggctgttc ctcttcattc tcgtggtggt cgcgtacggg
     3601 ttgacacggg gcgactggct ccagggtctc cttgcaggga tcacgctggc aatggccatc
     3661 ctccctgagg agttccccgt cgtgctgaca gtgttccttg cgctcggtgc atggcggctc
     3721 tcaaagatca atgtccttgc ccggcatgtc ccggcactgg aagccctcgg ggcaacgacc
     3781 gtgctctgca cggacaagac cggcacgctc accctcaacc ggatgaccgt cgcgaagtgc
     3841 gtggccggca gctccaccta tgactgcctg gccggggaag gcagcccgct tcccgaacca
     3901 ttccatgagc tcctcgagta cgccatcctc tcctgcaaga aagatccctt cgaccccatg
     3961 gagcaggcac tgctcaggac cgggaacagg gatctctccg ggaccgagca tctccaccgc
     4021 gattgggaac tgctgctcga ataccctctc tcccgcgagc ttctggcgat gtcaaacgtc
     4081 tggcggtcaa ggaaggggga cgagtacatc atcgctgcca agggagcacc ggaagcgatc
     4141 tttgatctat gtcatctcgg ggaagaggag accgaaggca tgaccgcccg ggtcgaagac
     4201 ctcgcctccc agggcctccg cgtcctcggc attgcaaaag cgcatttccg gatcgggcag
     4261 ctccccgaag gccagcacgc attccatttc acctacctcg gcctgatcgg gtttaccgac
     4321 ccggtgcgac cggacgcatc tgatgccgtt gccgaatgca ggaccgcggg gatccgggtg
     4381 gccatgatca ccggggatta ccctgcgact gcgaggaaca ttgcccgcca gatcgggctt
     4441 cctgactgcg acagggtcct tactggggct gaccttgact cgatgccccc ggataaaaga
     4501 gatgaggaga ttcactcttc ctctctcttt gccagggtag tccccgagca gaaactccaa
     4561 atcgtcgagt cgctgaaggc caggggggag atcgtcgcca tgaccggcga cggggtcaac
     4621 gatgccccgg cactgaaagc tgcgcatatc ggaatcgcga tgggtggcag ggggactgac
     4681 gtggccaggg aagcctcgtc gcttgtcctc cttgacgaca acttcgcttc tatcgttgcc
     4741 ggcatcagga cagggaggag gatctttgca aacctgcgga aggccatggc gtacatcatc
     4801 tcggtccacg tccccatcgc gggcctctcg ctcatcccgg tcctctccgg gtggccgctg
     4861 atcctcctcc ctgtccagat cgtgttcctg gagctgatca tcgatcctgc gtgttccgtc
     4921 gtcttcgaag cagagcaggc cgagccgggg atcatgcaga ggccgcccag ggacccgggc
     4981 gatcatgtcc tcgaccgccc cacggttgca attgctcttg cacagggcct cgttgccctc
     5041 tgcattgtca tcggcgtcac cctctacggg atgaagcagg ggctcgttgc ggaccagatt
     5101 cggacgatct cgtttgtcac tatcgtcatt gccaacctgg gcctcatctg cacaaaccgc
     5161 tcgtggtctg agagcattat tgccaccttc aggaagccaa acagggcgtt ctggtgggtg
     5221 atgggaggga ccgcattctt ccttgccgcg gtccttctca tccccggcct gagagcgctc
     5281 ttccggtttg ccccggtctc cccgtggtat ctccttgtct gctgcatggg aggcctgatg
     5341 actgttgcgt ggttcgagat ttacaaggtc acgcgtgccc gcgggtttgc atgagcgccc
     5401 gctgatcagg gccgtccgga gagaggttct gagaaaattt ttcggaatgg cacgatgcat
     5461 ggtgtatcag tcgggtgaga tcggccgggt cctggcagtc ggggcagtct aaaattaaaa
     5521 gggggccaat acgctctttg tttaagaaat actgcacata tgtgcagaat taaacaataa
     5581 catttaatga cagttctctc cattattata cccttccatg aaccaggaac ccccactgac
     5641 ccgtatccac tcgctatgct cgcagctcgc aaggcacccg gcattccttg gtgccctgat
     5701 cggaatcctg gcagcgttct cccaggccct cctcatcagt gctggcgggc ccgaggcgta
     5761 cgggttttgc gtcgcatgcc acacccgcga cctggtgaac ggcctgacca acatcatcgc
     5821 gggcacaaac ctagccctgg ccgccatctc gaagaactcc ctcctgccgg tcatgagcgt
     5881 ggcaggagtt ctcatcggcg gatacatctc ggccaccctt caccgggagc acaggataaa
     5941 gaaagggacc ataaaggact atttcatcta tttcttcgcc gggatccttg tcctcaacct
     6001 cgcgatgatc ttcggggggt gcccgtaccg ggcggccctc cggaccgggt acggggattt
     6061 cacggcagtc ctcttcatcg tggccatggc ggcgggcgtg gtcctgggtg catacctgct
     6121 gctcaaaaaa gcggagaggg aggacgcctg atggcgaccg gaatccttcc agttgatgct
     6181 gcagtcttct ttgtccccct cgtgacgctt accctgggcc ttctcatcgg ctaccttgcc
     6241 cagcggtccg ggtattgctc gatcggcggg ttccgggact acttcctgtt ccggcacacc
     6301 cgtctcctgt ccgggtacct cgccctgatc gtggcatcct ttgcggggta cttcctcttc
     6361 tggctcgtgg ttcccggtgc gatggagcat ttcttctggg cgaccaccag ggggcccttc
     6421 gtgccggttc ccggtgctcc cggaggcctg gttcccactg cctacctcat catctccatc
     6481 gtgacagggt tcctggtggg gctcatcgca gtcctcctgg gagggtgtcc catccgcctg
     6541 acggtcatgg catccgaggg gaattacagg gcagcctggt tctttgtcgg gatgtgcgca
     6601 ggttcgatca tctttgcaac gattgtgtca ggctgggtcg tctggttcat caacgcactc
     6661 gggtttggag cctgatcctc catggtctcc tccactttcc tcttctgcga cctggtgccg
     6721 ggtgagcgcc tgcgctggat tgcagagacg ctccgggcat ccaaggggac aggcggcgtc
     6781 cctctcagca tgacagcatt cctcaccggc gacgcgctct acagcctcgt tgacgcgagg
     6841 accagggatt cctggcgaac gcttgcagat cgcgacggcg tccgggtcat cgccgacggg
     6901 gacgaactcg gactccacgg cctgcgggat ttggttgcgt ccggatctcc atgggtcacg
     6961 gtcgcgggca gccaggacga ggccccgttc tggcagtccc tcgtctcatc ccttgtatca
     7021 gagtggaagg ggacgcagaa ggcaggcttt ctcctctgcg acggaccgta catgagccgg
     7081 gttaccgtct acatggtcag gttcctttcg gccgtccagg caggaggatt ttcacctgaa
     7141 ctctacacgt atcttgacgg tgtgcatgcc ctgcataacg gccagcgccc atccgagttt
     7201 gagaatatcg ggagggcaat cgcgggcatc tctgcatcat cagtccaggc cgggagggac
     7261 ccctggtttg ccgcctgctc ccggtgtgcg acggcaaggg gctactacca gatgaacccc
     7321 gggacagggt tctgcgagcc cgcctccgcc atcgaggaga tcgccatccg gccgctcaaa
     7381 gagatactct cgcggttttc cgggaacctg ccggtcgtat cgtctgcatc gggagacctg
     7441 gtgccggacg ggtggggagg agatcgcgtc cctcgcctcc tggtcttcat cgcacacccc
     7501 ccgtactgcg cggaatggac cttcggaggg ctctcgcttg cccttgccgc tgcgatgggc
     7561 gggatcccgg caacggtcat cttcatagag gatggggtgt acgcactcca cgggaaccac
     7621 gaagtccccg cgcacgacaa ggtcttcaat gtccaggaga tgatcgcggt cacgactgat
     7681 gttccggacc ttgagtattt cgtgcacggg ccttcgcttg acgaccgggg gatcgacctc
     7741 ttacccgggt tcccgactat cccgcgactg cgaaatgaag accttgcccg ggtgttcttg
     7801 aaatcggaga gcgacgggac cgcttcacgg ctcatattct tttaaaacga ggagagactg
     7861 acatgactga accaatggat ttcaccgaac tgacctgcac gaaccttatg atcaagctga
     7921 aaatcctgct gaacaagctc ccacagggcg atcgcgtcgc cttctttgca acgagggagc
     7981 aggtggacaa cacgtgctcg ccattctcag gccagggata ccaggtctca tgggaccagg
     8041 cggcggagaa ccggtatctc gtcaggctgg ggaagtaaga gaacaccgcg tggtctccgg
     8101 aactctctgg aatccacgcc cctttttgtc agggattcac gggagaccga gtctgcccat
     8161 tcaaggtatc ccggagtacc ttttcggacc ggggagaccc cttttcctcc cgaccaactg
     8221 gtcttggttc agtagattct cacctgtcaa agtgtttctg gggatctcat tccagagaaa
     8281 ttcctttgat tcacccaact cgcaatgaga cagtaatact gacgtggagg atcagggaac
     8341 gagggtaatc tcaccgtcct ctaccaggac cctgaacgtg ggttgcggtg ttgttgccgg
     8401 accgttcaac acctcccctg tcctgacgtt gaagaccgag ccatggcaga ggcagcggag
     8461 ggtctctcct tcgagcctgc cgtagccgag ccggcatgcc ccatgggcgc aactgttcgc
     8521 gagcgcatag atccctgatt ccaggtgtac cagcaggatc tcccggttat cgatcttaat
     8581 caccttcgta tccccgacag gaatgtcttt gaggggtccg agttttatgg ggtgtgcact
     8641 ctccattgtc aaccatttgt atataactat ttaaatattg gtaggggtgc atattatacg
     8701 ccattttcgt gattggcgga atattcaacg attaaatgga tgagatctgg aaaaaaagtg
     8761 gaaaagaaag cgcattcctt ccccttgtat ccctgcccgc aaccgggaac gacaccgtct
     8821 cccgttcccc gggctttgac ccgtggaaaa acagaaggga aatggtggag ttattccaga
     8881 cctttccggg cccgcaggag catggcgatc gcgagtcctg caagaccggc acaggcgagg
     8941 ggcgcagtcg gcgtgcgggt aacagttgtg ggagttggcg caggggtcat cgacggggag
     9001 aatttctgtg tccggttggc cgcccattcg gtcacgtaga cattgccggc ggcgtca
//
LOCUS       DAOQYR010000086         8850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_8392_length_8850_cov_10.293803, whole genome shotgun sequence.
ACCESSION   DAOQYR010000086 DAOQYR010000000
VERSION     DAOQYR010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8850
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8392_length_8850_cov_10.293803"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /gene="mcrG"
                     /locus_tag="PKM87_09360"
     CDS             <1..704
                     /gene="mcrG"
                     /locus_tag="PKM87_09360"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     gamma"
                     /protein_id="HNQ30313.1"
                     /translation="MDPGHKLEKVRDISDEDIVLLMGHRAPGAAYPTAHPPLAEQQEP
                     DCPIRKLVTPTDGAKHGDRIRYIQFADSMFFAPTHPYERSYLEAYRYRGVDPGTLSGR
                     QIVECRERDLEKYAKELVSTELFDSARTGIRGATVHGHSLRLAEDGMMFDMLQRCILD
                     KKGNMVKYVKNQIGEPLDKEVKVGKPMDEKWLKAHTTIYHSLGGVAFRDDAEYVEYVQ
                     RIHSLRTKYGFMPVE"
     gene            710..2416
                     /gene="mcrA"
                     /locus_tag="PKM87_09365"
     CDS             710..2416
                     /gene="mcrA"
                     /locus_tag="PKM87_09365"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     alpha"
                     /protein_id="HNQ30314.1"
                     /translation="MAKAKIERTQKLFLKALKGKFAEDPQSTNVVYAREGLKQSARKR
                     EFLESGKKMEMARGISMYDPVRCHLGGIPLGQRQLMTYEVSTTGVFVEGDDLHFVNNA
                     AMQQMWDDIRRTILVNMDLAHQTLQKRLGKEVTPETINEYLHVLNHAMPGAAVVQEHM
                     VETHPGLVDDCYCKVYTGDDEMIDDLEPQFVINVDKLFPAKQAAQLKAALGKSLWQAI
                     HIPTIVSRTCDGGTTSRWSAMQLGMSFIGAYRMCAGEAAVADLAFAAKHAGVIQMADI
                     LPARRARGPNEPGGIKFGHFADMVQADRKYPHDPARASLEVVGAGTMLFDQIWLGSYM
                     SGGVGFTQYATAAYTDNILDEYTYYGMDYIKQKYKVDWQNPSDKDKVKPTQEIVNDIA
                     TEVCLNGMEQYEQFPTLMEDHFGGSQRAGVLAAACGLSTSIATGNSNAGLNAWYLCML
                     MHKEGWSRLGFFGYDLQDQCGSANSLAIRPDEGAVGELRGPNYPNYAMNVGHQGEYAA
                     IVGGAHYGRGDAWSLNALIKVAFADPSLKFDFAEPRREFAKGAIREFMPAGERSLIIP
                     AR"
     gene            2734..3192
                     /locus_tag="PKM87_09370"
     CDS             2734..3192
                     /locus_tag="PKM87_09370"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNQ30315.1"
                     /translation="MDREGVGPHADADAETAAAVHGSRTPGRDHGGDRVKIAIASEGS
                     KVSEHFGHCSTYSLYSVENGVIFHREVLESPGHQPGVLPPFLASHDVTHVIAGGMGPR
                     AVELFQQHGIQVILGISGPIDLVAQEFIAGRIVPGVSSCHHTEGHSCDHE"
     gene            3418..4734
                     /locus_tag="PKM87_09375"
     CDS             3418..4734
                     /locus_tag="PKM87_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase glutamine
                     C-methyltransferase"
                     /protein_id="HNQ30316.1"
                     /translation="MLSPDLYTYGAMLIGGILQDAGMEVTLARSAGPCEEKILLLSLY
                     STQHLLDQKIKDSVRQHREKGGVCYAGGPVSSAPEMVLGELGVDAVVVGEGENAVVPL
                     VKRGISPDIPGIAYRDGDRVVVQPPVPPVTLARPLPLIPDDIGAQSIRGASAYIESHR
                     GCLGGCTFCQVPRFFGREIRSREIGEVLREVRAFRDKGARRISISGGTGSLYCYRDGN
                     LNPGAFIELLSGMAGIMGRENVSAPDIRVDCITDEILEAVGKYTIGWVFFGIESGSDR
                     ILSQMAKGVSVSQAKDAVEACRSHGLKVAGSFIVGYPTETADDYAASRDFIAEQGLDD
                     VFVSIAEPIPGTPLARLVLRTAREENPLFQRDTGEFKKLGLSIAEARCFDLMMEADMY
                     KPRLHVVTDQVFDLYLKEAKKQGNEIRAATDLLFRYSGSNLQQDCE"
     gene            4962..5861
                     /gene="mtrE"
                     /locus_tag="PKM87_09380"
     CDS             4962..5861
                     /gene="mtrE"
                     /locus_tag="PKM87_09380"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit E"
                     /protein_id="HNQ30317.1"
                     /translation="MESIAFGIGITALAGALATVAGAAENTESDIGSQGDPNSQVQLA
                     PQMGFLHRIFNKAVAGEPPAYGLWICLGAGIAWAFMAMQINPILAIVLGSALAVFIQG
                     VYATTAYLGRTASLAKFGQPVFVDVLKSMTTVTMAHAFVAIFTTVTMCHLINAALGHP
                     FPLPLLGLVWGIALGAAGSATGNPFYGKERQYQSQKFGAGVPISASGNIVRYAEAGER
                     NSLDNGWFSAKLGGPASGLCFGLIVFLEIWRTVLFEHVGAGWGAIIVGIIIILIFAVI
                     DRYIEVWARKNYGPYGKPAEEGS"
     gene            5864..6700
                     /gene="mtrD"
                     /locus_tag="PKM87_09385"
     CDS             5864..6700
                     /gene="mtrD"
                     /locus_tag="PKM87_09385"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit D"
                     /protein_id="HNQ30318.1"
                     /translation="MSAVGAKPQAGGAMDMPSTIAGIILLLVIIGATYFLVGSGLMFM
                     ALIGIIIGGLAIAFGVHFVPVGGAPAAMGQAPGIATGVAMLAAGAGLAGLFGGAWAAE
                     QGLVVAVITGAVGGGLMMAITCMFVTFIYVFGMGIPCASGKVAKDPITGDTQAEFKSQ
                     GTEGHGLPFVSYVGGVIGGLLGGGGGTLIYIMLLDVYKEALPGMMQALPAQILPIAVS
                     LAGIFAIGMFLVNAVLTAYNITGTIEGPHDPKFKRFPRALVASAVASALCGMVAILIV
                     AM"
     gene            6710..7558
                     /gene="mtrC"
                     /locus_tag="PKM87_09390"
     CDS             6710..7558
                     /gene="mtrC"
                     /locus_tag="PKM87_09390"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit C"
                     /protein_id="HNQ30319.1"
                     /translation="MTVKVEVIEGGVPHNTILAAGLVASLVLIYLTYANALTGTQLFS
                     FFGGLGAIAAIVWGSHAIKHLCSYGIGTGVPSAGMMAFGSGVIAMLFATKYGVAAPIV
                     AVVLAAIIGAILGFLSNNVLNMRIPVMIQSLIEMAIIGALVLMGFAAMMSGSFEFSPL
                     TSGTVTILGLSLPSYQASFIGGGLLAVAFMLGGIAIQHPFNACLGPNWTQDRMLTLAQ
                     ECGFLSMIAVAVMSMALISMAAALVSVIVALVGWYYTYAKYIELSKRDAAGWLDSKPI
                     PELEGH"
     gene            7560..7841
                     /gene="mtrB"
                     /locus_tag="PKM87_09395"
     CDS             7560..7841
                     /gene="mtrB"
                     /locus_tag="PKM87_09395"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit B"
                     /protein_id="HNQ30320.1"
                     /translation="MYVQVLPEYGLVVDPMIGVVTTAGESLAPVIEKVADMEAVADDI
                     VNMLSGKGNFLASFPNRENALPTAGAATAYWYGLAVGLFIAGVYVFHLM"
     gene            7852..8574
                     /gene="mtrA"
                     /locus_tag="PKM87_09400"
     CDS             7852..8574
                     /gene="mtrA"
                     /locus_tag="PKM87_09400"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit A"
                     /protein_id="HNQ30321.1"
                     /translation="MADKKSPASGWPIVQGDFHAGDANSPVAVMTCGSHLDEKGICDG
                     GAAITGSCKTENLGLEKVIANIISNPNIRFLLLCGTEVKGHLAGQTMAALHKGGVKDG
                     RVVGAEGAIPFIENLNDAAIKRFQEQVELVNIMEAEDLGAIKAKINELKGRDPGAFAA
                     DPMVVEVKEAAGGMEETGGVVQPMSGELALIHARMKVIEKMVTDIGYRDKFAAGVYSG
                     KIEGIMIGLIVSFAVLGFMLMG"
     gene            8580..8783
                     /locus_tag="PKM87_09405"
     CDS             8580..8783
                     /locus_tag="PKM87_09405"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit F"
                     /protein_id="HNQ30322.1"
                     /translation="MAEEGQKMAGPIRMVAIENMVENIRYKAQILARSNKYDSALLGS
                     GIIGFAAGVLTALILIVVPAVVM"
BASE COUNT         1841 a         2664 c         2648 g         1697 t
ORIGIN      
        1 acatggaccc cggccacaag ctggagaagg tccgggacat ctcggatgag gacatagttc
       61 tgctgatggg ccacagggcg cccggtgcag cctacccgac cgcccacccg ccgctcgccg
      121 agcagcagga gcccgactgt cccatccgga agttagtcac ccccacagac ggcgccaagc
      181 acggggaccg catccgctac atccagttcg ccgactcgat gttctttgca cctacccacc
      241 cgtacgagag gtcctacctc gaggcctacc gctaccgcgg cgtggacccg gggaccctgt
      301 ccggccgtca gatcgtagag tgccgcgagc gtgaccttga gaaatatgca aaggaactcg
      361 tcagcaccga gctcttcgac tctgcccgca ctggtatccg cggtgcgacc gtgcacgggc
      421 actccctccg ccttgccgag gacgggatga tgttcgacat gctgcagagg tgtatcctcg
      481 acaagaaggg caacatggtc aagtacgtca agaaccagat cggtgagccg ctcgacaagg
      541 aagtcaaggt cggcaagccg atggacgaga agtggctcaa ggcgcacacc accatctacc
      601 actcgctcgg cggcgtagcc ttccgtgacg acgcagagta cgtcgaatac gttcagcgta
      661 tccactcgct gaggaccaag tacggcttca tgccggtgga gtgattgaca tggcaaaagc
      721 aaagatcgag aggacgcaga aactcttctt aaaggcactg aaggggaaat tcgcagaaga
      781 cccccagtcc accaacgtgg tctacgcccg cgagggtctc aagcagtccg ccaggaagag
      841 ggagttcctt gagtccggca agaagatgga gatggcccgt ggcatttcca tgtacgaccc
      901 tgtccgctgc cacctcggcg gtatcccgct cgggcagcgc cagctgatga cctatgaggt
      961 ctcgacgaca ggcgtattcg tggagggcga cgacctgcac ttcgtcaaca acgctgccat
     1021 gcagcagatg tgggacgaca tccgcaggac catcctcgtc aacatggacc ttgcccacca
     1081 gaccctgcag aagcgtcttg gcaaggaagt gacccccgag accatcaacg agtacctgca
     1141 cgtgctgaac cacgcgatgc ccggcgcagc ggtcgtccag gagcacatgg tggagaccca
     1201 ccccggactc gtcgacgact gttactgtaa ggtctatacc ggcgacgacg agatgatcga
     1261 cgacctggag ccccagttcg tcatcaacgt cgacaagctc ttcccggcca agcaggccgc
     1321 ccagctgaag gccgcactcg gcaagtcgct ctggcaggca atccacatcc cgaccatcgt
     1381 ttcgaggacc tgtgacggag gcaccacctc ccgctggtct gcgatgcagc tcggcatgtc
     1441 cttcatcggc gcctaccgca tgtgcgccgg agaggccgcc gtcgccgacc tggcgttcgc
     1501 cgcaaagcac gccggtgtga tccagatggc cgacatcctg cccgcacgcc gtgcccgtgg
     1561 cccgaacgag cctggtggca tcaagttcgg acactttgcc gacatggtcc aggccgacag
     1621 gaagtacccg cacgaccccg cccgcgcctc cctcgaggtc gtcggtgcag ggacgatgct
     1681 cttcgaccag atctggctcg ggtcctacat gtccggaggc gtcgggttca cgcagtacgc
     1741 gactgccgcc tacaccgaca acatccttga tgagtacacc tactatggga tggactacat
     1801 caagcagaag tacaaagtcg actggcagaa cccaagcgac aaggacaagg tcaagccgac
     1861 ccaggagatc gtcaacgaca tcgcaaccga ggtctgcctg aacggaatgg agcagtacga
     1921 gcagttcccg accctgatgg aggaccactt cggcggctcc cagcgtgccg gtgtgctcgc
     1981 cgctgcctgc ggtctgtcca cctcgattgc gaccgggaac tccaacgccg ggctgaacgc
     2041 ctggtacctg tgcatgctga tgcacaagga aggctggtca cgtctcgggt tcttcggcta
     2101 cgacctgcag gaccagtgcg gttccgcaaa ctcgctcgcg attcggcccg acgagggcgc
     2161 tgtcggagag ctccgcggac ccaactaccc gaactacgcc atgaacgtgg gacaccaggg
     2221 agagtacgcc gctatcgtcg gcggtgctca ctacggacgc ggagatgcct ggtcactcaa
     2281 cgcgctgatc aaagtcgcct tcgccgaccc ctcgctcaag ttcgacttcg ccgagcccag
     2341 gcgcgagttc gccaagggtg caatccgcga gttcatgccc gccggagagc gctcgctgat
     2401 catacccgcg cggtaaggtc agaaaccacc ccctttttct ttttttcgtc ttcacgccgc
     2461 gatagtggct tggatgcgct cctgcccggt tcaacaggtt tatatagatt tttactcata
     2521 tgagtaatta ttacacgcgc gaacccgtgc gtgaaggaga catgataatg cctggacttg
     2581 atggaagcgg gccccgtgga agaggggcaa tgacagtgag gggcttcggg cgctgcgctg
     2641 ctgcagcagt cccggttcct cctgatgcgg cccgcgatgt cgcgcccgag atggaggatt
     2701 caacgcagtt cccccaggaa agccggggaa catatggacc gggaaggagt gggaccgcac
     2761 gcggatgcgg acgcggaaac ggccgcggcc gttcacggat cacggacacc cggcagggat
     2821 catggcggtg acagggtgaa gatcgcgatt gcaagcgaag ggtcgaaagt ctccgagcac
     2881 ttcggccact gcagcacgta ttccctctat tcggttgaga acggcgtcat attccacagg
     2941 gaggtgctgg agagccccgg ccaccagccc ggcgtcctgc cgccgttcct tgcctcccat
     3001 gatgtgaccc acgtgattgc gggaggcatg ggaccccggg cggtcgaact cttccagcag
     3061 cacggcatcc aggtgatcct cgggatcagc gggccgatag acctggtcgc ccaggagttc
     3121 atcgcgggcc ggatcgtgcc cggtgtctcg agctgccacc ataccgaagg ccattcctgc
     3181 gaccacgagt gagaaatccc ctcctttcag gagtgctcca aaaatcctcg ttttttactg
     3241 gaattcaagg ataagggcat atcctgcacg attcactcct cacgccctcc tctcacggtg
     3301 tgctcaagaa atcccccttt tgacatcagg tgggtgaaaa atcattacat tttatctttc
     3361 tttccggcca agtgactgta ggtacccgcc tggaggaaga ttgcactgaa cgtccggata
     3421 ctctcccccg atctctacac ctacggcgcc atgctgatcg gcgggatatt gcaggacgca
     3481 gggatggagg tgaccctggc acggtcggct gggccatgcg aagagaagat cctgctcctt
     3541 tcactctatt ccacgcagca tctcctggac cagaagataa aggacagcgt gcggcagcac
     3601 agggaaaagg gcggggtctg ctacgcggga ggacctgtct cttctgcccc ggagatggtg
     3661 ctcggcgagc tcggggtcga cgcggtcgtg gtcggcgaag gggagaatgc cgtggtaccc
     3721 cttgtgaaac gggggatatc tcccgatatt cccgggatcg cgtacaggga cggggaccgg
     3781 gttgtggtcc agccgcctgt tccacctgtc acccttgccc gcccacttcc cctgattcca
     3841 gacgacatcg gggcccagag catcagggga gccagcgcct acatcgagag ccaccgggga
     3901 tgccttggcg gatgcacgtt ctgccaggtc ccgcgtttct tcggccgcga gatccggtca
     3961 agggagatcg gagaggtgct ccgcgaggtc cgggcgttcc gggacaaggg agcccggcgc
     4021 atctctatct cgggggggac cggctctctc tactgctacc gtgacgggaa cctcaacccc
     4081 ggcgcattca tcgagctcct ctcgggcatg gccggcatca tggggcggga aaacgtctct
     4141 gcacccgaca tacgggtaga ctgcatcacg gacgagatac tggaagccgt cgggaaatac
     4201 accatcgggt gggtattctt cggcatcgaa tcggggagcg accggatcct ttcccagatg
     4261 gcgaagggtg tcagtgtctc ccaggcgaaa gatgcggttg aggcatgcag gagccacggc
     4321 ctgaaagttg ccggaagttt tattgtcggg tatcccaccg agaccgcgga cgattatgca
     4381 gcgagcaggg atttcatcgc ggaacagggc ttagacgacg tatttgtaag cattgccgaa
     4441 cctatccccg gcacccctct cgcccggctc gtccttcgca ccgcaagaga agaaaacccg
     4501 ctcttccagc gggataccgg ggagtttaaa aaactgggcc tctcgatcgc cgaagcccgc
     4561 tgttttgacc tgatgatgga agccgacatg tacaagccac gcctccatgt tgttaccgac
     4621 caggtctttg acctctacct gaaagaagca aaaaagcagg ggaacgagat ccgtgccgcc
     4681 accgacctgc ttttcaggta ttccggttcc aatctacagc aggattgcga ataacacaac
     4741 tttatttaac ttaactaaaa tccagatgct aaagagtccc gaaaagccgt ttttttgccg
     4801 attccggcac attagccgcg tatataagca aaaatttcga aacctttaac tcttccatca
     4861 tcgactgtta gatgaaccac aaagggtttg tactcgatgt gagtggcaat gtgtatcgaa
     4921 cactctcgtg cactcttcta cgcacgcagg gaggaggcta aatggaaagt attgcgtttg
     4981 gcattggaat aactgcattg gcaggagctc ttgccacagt ggccggtgct gcggagaaca
     5041 ctgaatctga tatcggatca cagggtgacc cgaactcgca ggttcagctc gccccccaga
     5101 tggggtttct tcaccgcatc ttcaataagg ccgttgccgg tgagccaccc gcctatgggc
     5161 tctggatctg cctcggtgct ggtatcgcct gggcattcat ggcaatgcag atcaatccga
     5221 tacttgcaat cgtgctggga agcgccctgg cggtattcat ccagggcgtg tatgcgacaa
     5281 ccgcatacct cggcaggacg gcaagtctcg cgaagtttgg tcagcccgtc tttgtagacg
     5341 tgctgaagtc catgaccacg gtgaccatgg cacatgcatt tgtcgccatc ttcaccacgg
     5401 tgaccatgtg tcacctgatc aacgccgcgc tcggccaccc cttcccactg cccctgctgg
     5461 ggctggtgtg gggtattgcg ctcggcgctg ccggatctgc aactggaaac cccttctatg
     5521 gaaaggagcg gcagtaccag tcccagaaat tcggggccgg tgtgcccata tccgcatccg
     5581 gtaacatcgt ccgctacgcc gaagcgggtg agaggaactc ccttgacaac gggtggttct
     5641 ctgcaaagct cggcggccct gcatcagggc tctgtttcgg cctgatcgta ttcctcgaga
     5701 tatggcgaac cgtgctcttt gagcacgtgg gtgccggctg gggagccatc attgtcggga
     5761 tcattatcat cctgatcttc gcggtcatcg accgctatat cgaagtctgg gcccgcaaga
     5821 actacggacc ctatggaaag cctgccgagg agggatcctg aagatgagcg ctgttggagc
     5881 aaaaccacag gccggagggg caatggacat gccctccacc atcgcaggca tcatcctcct
     5941 gctggtgatc atcggtgcca cctacttcct ggtagggtca ggcctgatgt tcatggctct
     6001 catcgggatc atcatcggcg gactcgccat cgccttcggc gtccacttcg tcccggtcgg
     6061 cggtgcaccc gcagccatgg ggcaggcacc tggtatcgcg accggtgtcg ccatgctcgc
     6121 tgccggtgcg gggcttgcag gtctcttcgg aggcgcctgg gccgcagaac aggggctcgt
     6181 ggttgcagtg atcacagggg cggtcggcgg cggtttgatg atggctatca cctgcatgtt
     6241 cgtcaccttc atctacgtct tcggcatggg aatcccctgt gcctcgggaa aggtcgccaa
     6301 ggacccgatc accggggaca cgcaggccga gttcaagtcc cagggaactg agggtcacgg
     6361 actcccgttc gtctcctacg tgggcggcgt catcggcggc ctccttggag gcggcggcgg
     6421 gacgctcatc tacatcatgc tcctggacgt gtacaaggag gctctccccg gcatgatgca
     6481 ggcgcttcct gcccagatac tgcccatcgc agtgagcctg gcaggcatct tcgccatagg
     6541 catgttcctg gtgaacgcgg tgctcaccgc gtacaacatc accggtacga tcgaaggccc
     6601 gcatgacccc aagttcaagc ggttcccccg ggcactcgtc gcctctgcag tggcatcagc
     6661 cctctgcggg atggtcgcga tcctgatcgt ggcaatgtga ggtgcagaaa tgacagtaaa
     6721 ggttgaagta atcgagggag gcgttcccca caacaccatc ctggcagcag gcctcgtcgc
     6781 gtcgctggta ctcatctacc tgacgtatgc gaacgccctt accgggaccc agctcttctc
     6841 gttcttcggg ggactgggcg cgattgccgc cattgtatgg ggaagccacg ccatcaagca
     6901 cctgtgcagc tacggtatcg gtaccggcgt gccttccgca ggcatgatgg cattcggttc
     6961 cggcgtgatc gccatgctgt ttgccaccaa gtacggggtg gccgcaccga tagtcgcagt
     7021 cgtcctggcc gcaatcatcg gagcaattct cgggttcctg tccaacaacg tcctgaacat
     7081 gcgcatcccg gtcatgatcc agtcgctgat cgagatggca atcatcggtg cactggtcct
     7141 gatgggattt gcagccatga tgtccggatc gtttgagttc tctccgctga cctctggcac
     7201 cgtgaccatc ctcgggctct cgctgccgag ttaccaggca tccttcattg gaggaggtct
     7261 gctcgcagtg gcattcatgc tcggcgggat tgcaatccag cacccgttca atgcatgcct
     7321 cggtccaaac tggacccagg acaggatgct cacgcttgca caggagtgcg ggttcctctc
     7381 catgatcgcc gtggcagtga tgtcgatggc tctgatcagc atggcggccg cactggtatc
     7441 cgtcatcgtg gcactcgtgg gctggtacta cacctacgcg aagtacatcg agctctcgaa
     7501 gcgcgatgca gcaggatggc tggattcaaa accgataccg gaactggagg gacactgata
     7561 tgtacgtcca ggttcttccg gaatacggac ttgtggtcga cccgatgatc ggggtcgtga
     7621 ccaccgcggg cgagtcgctt gcaccggtga tcgagaaggt cgccgacatg gaagccgtcg
     7681 ccgacgatat cgtgaacatg ctctccggaa aaggcaactt cctggcttcg ttcccgaaca
     7741 gggagaacgc acttccgacc gcaggcgcag caaccgccta ctggtacgga ctggcagtcg
     7801 gcctcttcat cgcaggggtg tatgtattcc atctgatgtg aggtgaaaga gatggcagac
     7861 aagaaatcac cagcaagcgg atggccgatc gtgcagggag acttccatgc aggcgatgca
     7921 aacagccccg tggccgtgat gacctgcggg tcccacctcg acgagaaggg catctgtgac
     7981 ggcggggccg ccattacagg gtcctgcaag accgagaacc tcggtctcga gaaggtcatc
     8041 gccaacatca tctccaaccc caacatccgg ttcctcctgc tctgcgggac cgaggtcaag
     8101 ggtcacctcg ccggccagac catggccgcg ctccacaagg gcggggtaaa agacggcagg
     8161 gtcgtcggtg cagagggtgc gatccccttc atcgagaacc tcaacgatgc agccatcaag
     8221 cgtttccagg agcaggttga actggtcaac atcatggagg ccgaggacct cggcgccatc
     8281 aaggccaaga tcaacgagct caaggggcgt gacccgggcg catttgccgc agaccccatg
     8341 gtcgtggagg tcaaggaagc cgcgggcggg atggaagaga ccggtggggt cgtccagccc
     8401 atgtcaggcg aacttgccct catccacgcg cgaatgaaag tgatcgagaa gatggtgacg
     8461 gacatcgggt accgcgacaa gttcgcggcc ggggtctatt ccggtaagat cgagggtata
     8521 atgatcggtc tcatcgtgtc gttcgccgtc ctcgggttca tgctgatggg gtgaacaaaa
     8581 tggcagaaga aggacaaaag atggcaggac ctatcagaat ggtcgccatc gagaacatgg
     8641 tggagaacat ccgctacaag gcgcagatcc tggcgagatc gaacaagtac gactccgcac
     8701 tcctgggctc cggcatcatc ggatttgccg cgggagtact gaccgcactg atcctgatcg
     8761 tcgtgcctgc cgtggtcatg tgaggtgaaa gagaaatggc agacaagaaa tcaccagcaa
     8821 gcggatggcc gatcgtgcag ggagacttcc
//
LOCUS       DAOQYR010000087         8845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_8401_length_8845_cov_8.675654, whole genome shotgun sequence.
ACCESSION   DAOQYR010000087 DAOQYR010000000
VERSION     DAOQYR010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 8845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8845
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8401_length_8845_cov_8.675654"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PKM87_09410"
     CDS             <1..602
                     /locus_tag="PKM87_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNQ30323.1"
                     /translation="FPEVLGEWQGLGYNRRAIFLHRIARLVVDEYAGRLPDDEGTLSS
                     FPGIGEATAASLVAFVFNRPSIVIETNVRRVFLHCFFEGHSVVRDREIRPLVAATCDR
                     ESPREWYYALMDLGSALKKRIPNPNRRSAHYRKQAAFEGSDRMVRGRILAILVREGAV
                     RESDLPPMLGIDRNRAAGILETLEKEGFLKREGGTAVLL"
     gene            complement(636..815)
                     /locus_tag="PKM87_09415"
     CDS             complement(636..815)
                     /locus_tag="PKM87_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30324.1"
                     /translation="MEKNVKKQISLICFAAGFVLLLIQDLKGFSTIDLGFSWPWAIMF
                     YCGLLLMVIGYYLRG"
     gene            complement(969..1568)
                     /locus_tag="PKM87_09420"
     CDS             complement(969..1568)
                     /locus_tag="PKM87_09420"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HNQ30325.1"
                     /translation="MKIVGVGCGPGMLTEEAIAAISRASLVYGSPRAIDLARSHIPGG
                     CTVREIEDYKSLRTLPVDAVVLSTGDPMLAGLGYLEGEVIPGISSLQITLARLHIPMT
                     QISVVAAHGRDHDAAIRETVAEISRGRTVFLLADPGFEVPHLAKHFLPPPPGLTITLC
                     EQLGYPDERVVTGTPDAPPVPCNDLFVILVNPGAMRRSH"
     gene            complement(1565..2608)
                     /locus_tag="PKM87_09425"
     CDS             complement(1565..2608)
                     /locus_tag="PKM87_09425"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HNQ30326.1"
                     /translation="MPSNTQPDPDRTTITDPVTGFVYPEEWVSACRDPGLRALASSGL
                     GVLTSSGRVLRRGFTTGTTAAAACGAAILSLRGPVREVRVRIPCGLLVSVPARSEGGH
                     AEADKFAGDYPGDATAGVTFFADARPRGQGIVLIAGAGIGRFARDTPRNSAGDPAISQ
                     AALQCIMDAIDSAAREIGLSGVEVSLSVPKGEIIAAETLNPKIGILSGISVLGTTGLV
                     EPWDDHLTESMLERVQSAERLVITTGRLGLRYSRLLFPDHEVVLAGARIREALERARG
                     EVILCGLPGLVLKFLDPDILEGTGCATVEELSGRPGFGRLVAGVFEKTRTAFPGLRVV
                     VLSREGEVLGDSG"
     gene            complement(2580..3248)
                     /locus_tag="PKM87_09430"
     CDS             complement(2580..3248)
                     /locus_tag="PKM87_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HNQ30327.1"
                     /translation="MPDGSSPRGDTTGNTYTDLGATTKEAYQISETSRRLARKTIGNS
                     DYEDRVRQRCSIAVGDFSMASLIRFTGDPIPAGLRALEFQALIITDIRMVQVGIQKKG
                     HASEVICALDHAGSAAEKGITRSRAGILALGERIDGAVVVIGNAPSALLSLCDLIKEG
                     RTPSLVVGTPVGFVNAAESKELLRTLPVPSISNIGTRGGTPVAVAAINEIITIHAEQH
                     PTRP"
     gene            complement(3196..3978)
                     /gene="cobJ"
                     /locus_tag="PKM87_09435"
     CDS             complement(3196..3978)
                     /gene="cobJ"
                     /locus_tag="PKM87_09435"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HNQ30328.1"
                     /translation="MPSHGEGRGVLYIVGSGPGSPDQMTGKALQAIRDAEYVLGNDSY
                     LTPLSPLIEGKTVIRSSMGKEVERAREAVTLARDHVVAMVSGGDPGVYGMASIVLEVA
                     ERSGSAARIEVIPGVTAATAAASRLGSPLSGDFAVVSLSDLLTPMEVIEKRLEHAFAM
                     GIPVVLYNPRSRGRPYNFARAMEIAASHRESSTPLGLVKNAYRDGEEIIITTLGQVAT
                     IEDQVDMHSAVIVGGTESRIWMEGSHARWIITPRGYHRKYLY"
     gene            complement(3962..4831)
                     /gene="cbiG"
                     /locus_tag="PKM87_09440"
     CDS             complement(3962..4831)
                     /gene="cbiG"
                     /locus_tag="PKM87_09440"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HNQ30329.1"
                     /translation="MNRTVIALERFEDAGRRVAEFLHAELILYSDEAFPIAFSRSSAI
                     VAVMASGIAVRAIAPLLNDKWTDPAVVVVDPGLCFAIPLVGGHHGANDLAWELAGLGI
                     TPVITTATEREGKAAVEVVAAREGCRVLNRDSTVSVNAALLDGEVPVHVVSGPAMVVA
                     GPGVALLARDGEYAVGLGCRRGTPAREVEDAVRKALGVSGISADEVSVYATTEKKACE
                     PGIVEGVRALRGTLIYLDDKILARFPPQSPSRAGKVGLAGVAEPAAMAASMRKELVMR
                     KTVYGEVTVAIAR"
     gene            complement(4828..5544)
                     /gene="cobM"
                     /locus_tag="PKM87_09445"
     CDS             complement(4828..5544)
                     /gene="cobM"
                     /locus_tag="PKM87_09445"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HNQ30330.1"
                     /translation="MAKVCFVGAGPGDPDLITVKGLRLLEEADVLVYTGSLVNPGLVE
                     RSHARIKLDSHGMKLEEIVDAMVLHARSGARVVRLHSGDPALYGAIVEQMQGLSHKGV
                     DYEVVPGVSSLFAAAAALRTQLTLGGVAESVIVTRPAGATLEVDEIPALSTHKATMVF
                     FLGTAHLEEITSRVACPQETPAAVVYHASWPDQKVVRGTVSDIAAKARAAGIDKTALL
                     IIGGVLDPVHSGHHRSQLYS"
     gene            complement(5546..6151)
                     /locus_tag="PKM87_09450"
     CDS             complement(5546..6151)
                     /locus_tag="PKM87_09450"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HNQ30331.1"
                     /translation="MLVGLGLGPGDPELLTLRAVRVLQEADAVFVPGRLAQNLVAPYR
                     DAEVLDFPMTGDEGRIRSCMERNADRIAGIAASGTAVLALIGDPNFFSTFTRLCEVMA
                     QRYPDIPCRTEPGISAITAFAAAAGISLSEGFVVTDRGEPRCQILLKVKRPKETAERL
                     RKEGFRRFVLVERMYLSDMRIYRDDELPETCDYLSIMYAGV"
     gene            complement(6142..6675)
                     /gene="cbiT"
                     /locus_tag="PKM87_09455"
     CDS             complement(6142..6675)
                     /gene="cbiT"
                     /locus_tag="PKM87_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HNQ30332.1"
                     /translation="MSLPGGPTKDEVMAVSLFKLDLRPGDRFIDIGCGTGKVSIHAAR
                     QAGSVCAIDIRSEAIEYARNEAERAGVENIEFIEGNAADLIPGLPHPDAAFLGGSRDM
                     GRILHLLAGMDVRSIVVNAVKIDTINTAITVMRDLGIFREAVLVQISHASPLGAGHMW
                     KPAHPVAVIVGGAAGCS"
     gene            complement(6905..7123)
                     /locus_tag="PKM87_09460"
     CDS             complement(6905..7123)
                     /locus_tag="PKM87_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNQ30333.1"
                     /translation="MKESQEFIRETCRVIPAHDLMAEAFDMACSCRITIYDALFLAAA
                     ARCGFPLVAADSRLYSAARKNFSIRLIR"
     gene            complement(7679..7912)
                     /locus_tag="PKM87_09465"
     CDS             complement(7679..7912)
                     /locus_tag="PKM87_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30334.1"
                     /translation="MQASSVRLDKTTKEKLDLLRSYPKESYDSIIRRLAESRIDEEPL
                     TDEDRNEIDEALTEIRKGHYYTHDQVKRELGLS"
     gene            complement(8354..>8845)
                     /locus_tag="PKM87_09470"
     CDS             complement(8354..>8845)
                     /locus_tag="PKM87_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNQ30335.1"
                     /translation="GGDPFLFGRGGEELEVMREHGVPVELVSGVTSALAVPGAVGIPL
                     THRKYASQVTILTGHEEPGKTESGLDWENLARNPGTLVILMGVKNIPDITRSLVDNGK
                     DPDTPVAVIERGLRSDQRVTVGNLRTIADIAKSRNVRPPAIIVIGDVVRLYREDDPGL
                     VPL"
BASE COUNT         1692 a         2691 c         2636 g         1826 t
ORIGIN      
        1 cgtttcccga ggtgctcggg gagtggcagg gactcgggta taacaggagg gccattttcc
       61 tgcatcggat cgcgcgtctg gtggtggatg aatatgcggg ccgcctcccg gacgatgaag
      121 ggacgctctc gtcgtttccc ggcattggcg aggccacggc ggcatcgctc gtggcattcg
      181 tgttcaacag gccgtctatc gtgattgaga ccaatgtgag gagggtcttt ctccattgct
      241 tcttcgaggg ccatagcgtt gtgagagacc gggagatccg tcctctcgtc gccgcaacct
      301 gtgatcgtga gagtccgcgg gagtggtact atgccctgat ggatcttgga agcgcgctca
      361 aaaagcgcat cccgaacccc aaccgccgaa gtgcccatta ccggaaacag gccgccttcg
      421 aaggctcgga caggatggta agggggagga tcctcgcaat cctggtcagg gagggggcgg
      481 tcagggaatc agacctcccg ccgatgctcg ggatcgacag gaacagggcg gcgggaatcc
      541 ttgagactct tgaaaaggag ggcttcctga agagggaagg cggaacagca gtacttcttt
      601 gaactctccg cccccacgcg ccccccggca ccacgttatc cccggaggta atacccgatt
      661 accatcagca gcaggccgca gtagaacatg atcgcccagg gccacgagaa cccaaggtca
      721 atggtcgaga accccttgag gtcctggatg aggagcagga caaagccggc tgcgaaacat
      781 atcaggctga tctgcttttt gacattcttc tccatggaaa atctcctgtg tggacagttg
      841 cctgtattga ggtatgaggt ggttggatat attcctcttg accagaatgt cacaataact
      901 ggggaaaggt ccagggcaga agaacttttt catgtctccc ctctcatcga agcggataaa
      961 gggtgcgctc agtggcttct tctcatggca ccggggttga ccaggatgac aaagaggtca
     1021 ttgcagggca cgggcggggc atcgggtgtc ccggtcacca cccgctcgtc tgggtatccc
     1081 agctgctcgc agagggtgat ggtgagtccg ggcgggggcg gcaggaagtg cttcgcgagg
     1141 tgagggactt cgaaccctgg gtctgccagg aggaagactg tccttcccct ggaaatctct
     1201 gcaacagtct ccctgatagc cgcatcatgg tccctcccgt gggccgcaac taccgaaatt
     1261 tgcgtcatcg ggatatgcag ccgggcaagg gtgatctgga gggaggagat gccgggaatc
     1321 acctcgcctt caaggtatcc gagacccgcg agcatggggt cgccggtgga gaggacgacg
     1381 gcatcaactg gaagggtccg gagagatttg tagtcctcga tctccctgac cgtgcatccc
     1441 ccggggatgt gagatctcgc gaggtcgatg gccctcgggg aaccgtatac gagggacgcg
     1501 cgggatattg ccgcgattgc ttcttctgtg agcatccccg gcccacagcc cactccgacg
     1561 atcttcatcc gctgtccccc agcacttctc cctctcgcga gagaacaacc acccggagac
     1621 cgggaaacgc ggtcctggtt ttttcgaaaa cccctgcaac gagcctgccg aatccgggcc
     1681 tgccggaaag ctcctccacc gtcgcgcacc cggtcccttc gaggatatcc ggatcaagga
     1741 atttcaggac aagtcccggg aggccgcaga ggatcacctc tcccctcgca cgctccaggg
     1801 cttcccgtat ccttgccccg gcgagcacca cctcgtggtc ggggaagagg agccgggaat
     1861 agcggaggcc aagcctcccg gtggtgatca ccaggcgttc tgcactctgc actctctcga
     1921 gcatcgattc tgtgaggtgg tcgtcccacg gctcgacgag accggtggtc ccgagtacag
     1981 atattcccga aaggatcccg atctttgggt tcagagtctc tgccgctatg atctcaccct
     2041 tcgggacaga gagagagact tcaacaccgg agaggccgat ctccctggcc gcgctgtcga
     2101 tggcatccat gatgcactgg agggccgcct ggctgatggc agggtccccc gcgctgttcc
     2161 ggggggtgtc cctggcaaac cggccgatcc ccgcccctgc aatgaggacg ataccctggc
     2221 cacgcggcct tgcatctgca aagaacgtca ctcccgcagt ggcatcgccg gggtagtccc
     2281 cggcgaattt atcagcttct gcgtgtcctc cttcactccg ggcgggaacg gataccagga
     2341 ggccgcaggg aatgcgaacc cgcacctctc gcacgggtcc gcgaagggaa agaatcgccg
     2401 cgccgcacgc cgccgctgcc gtcgtgccgg tagtaaaccc cctcctcaac acgcgcccgc
     2461 tcgacgtgag caccccgagg ccggatgaag cgagggctct caatcccggg tcccggcagg
     2521 cgctcaccca ctcctccggg tatacgaatc cagtcacggg atcggtgatg gtcgtcctat
     2581 cagggtcggg ttgggtgttg ctcggcatgg atggtgatga tctcgttgat tgccgcgacg
     2641 gcaacggggg tccctcccct cgtcccaatg tttgagatcg agggtacagg cagggttctc
     2701 aagagttctt ttgactcggc ggcgttcacg aacccgacgg gtgtgcctac cacaagggag
     2761 ggagtacgcc cttccttgat gaggtcgcag agggagagga gggctgacgg agcgtttcct
     2821 atcactacca ccgcgccgtc gatcctctcc cccagggcaa ggatacctgc cctcgaccgg
     2881 gtgatccctt tctcggccgc cgaacctgca tggtcgaggg cgcagatgac ctcgcttgcg
     2941 tgccctttct tctggatccc gacctgcacc attcggatat cggtgatgat gagggcctgg
     3001 aattccaggg cgcggagccc tgcgggaatg ggatctcccg tgaacctgat gagcgaggcc
     3061 atcgagaagt ctccgactgc gatggagcag cgctgccgca ccctgtcctc gtagtcgctg
     3121 tttccgatag tctttctcgc aagtctcctg ctcgtctccg aaatctggta ggcctccttc
     3181 gtggtggccc caaggtcagt ataggtattt cctgtggtat ccccgcgggg tgatgatcca
     3241 tctggcatgc gatccctcca tccaaatcct gctctctgtt cctcccacga tcaccgcgct
     3301 gtgcatgtcc acctggtctt cgatcgtggc gacctgcccg agcgtagtga tgatgatctc
     3361 ctcgccatcc ctgtaggcat tcttcaccag tccaagaggg gtcgaggatt cccggtgaga
     3421 ggcggctatc tccattgcac gggcaaagtt gtaagggcgg cccctgctcc ggggattgta
     3481 tagtactact ggtatcccca ttgcaaaggc atgttcgagc cgcttctcga tcacctccat
     3541 gggcgtgagg aggtccgaga ggctgacgac ggcaaagtcc ccggagagag gagacccgag
     3601 ccttgaagcc gcggcagtcg ccgcggtgac gccgggaatc acctcgatcc tggcagcgga
     3661 gccggagcgt tccgcgacct caaggacgat gctggccatc ccgtatactc cggggtctcc
     3721 tccgctgacc atcgccacca catgatcccg tgccagcgta accgcctccc ttgcccgctc
     3781 cacctctttt cccatgctgc tccggatcac cgtctttcct tcgataaggg gtgacagggg
     3841 cgtaaggtac gagtcgttcc cgaggacgta ctccgcgtcg cgtattgcct ggagcgcttt
     3901 tccggtcatc tggtcgggtg aaccgggacc cgaacccacg atgtagagga cgccccgccc
     3961 ttcaccgtgc gatggcaacg gtcacctctc cgtatacagt ctttctcatc acgagctcct
     4021 ttctcattga tgcggccatt gccgcaggtt ccgccacccc ggcgagaccc accttcccgg
     4081 cacgggaggg ggactgcggg gggaacctcg cgagtatttt gtcgtcgagg tagatgaggg
     4141 tcccgcgaag agccctcacc ccctcgacaa ttcccggttc gcaggctttc ttttcggtgg
     4201 tggcatagac gctcacctca tcggcagata ttccggatac cccgagggcc tttctgaccg
     4261 catcctcgac ctctcttgca ggagtgcccc tgcggcagcc gagcccgacg gcatactcgc
     4321 cgtcccttgc aaggagcgcg acgcctggcc ccgcgacgac cattgccggg ccggacacga
     4381 cgtgcaccgg tacttccccg tcaaggagtg ccgcattgac cgaaacggtg gaatcacggt
     4441 tcaggacacg gcacccttcc ctggcggcaa ccacttcgac ggcagccttc ccctcacgct
     4501 cggtggcggt cgtgatcacc ggggtgatcc caagcccggc gagttcccat gcaaggtcat
     4561 tggccccatg gtgcccgcct accaggggga ttgcaaagca gagcccgggg tccacgacaa
     4621 ccaccgcggg atcggtccac ttgtcgttga ggaggggggc gattgcccgg actgcgatcc
     4681 cggatgccat caccgcgaca atggcgctcg agcgggagaa agcgatgggg aatgcttcgt
     4741 ccgagtagag gatcagctcg gcgtgcagga actcggcaac cctcctcccc gcatcctcga
     4801 atctttccag ggcgatgact gtcctgttca tgaatagagt tgcgacctgt ggtggcccga
     4861 atggacgggg tcgagcactc cgccgatgat gagaagcgcc gtcttatcga tgcccgccgc
     4921 tcttgccttc gcagcgatat cggaaacggt ccccctcacc accttctggt cgggccagct
     4981 tgcatggtat acgactgcgg caggcgtttc ctgcgggcac gcgacgcgtg atgtgatctc
     5041 ctccaggtgg gcagtgccaa ggaagaagac catggtggct ttgtgggtgg agagggccgg
     5101 gatctcgtct acctcgagtg tggccccggc ggggcgggtg acgatcacgc tctctgcgac
     5161 accgccgaga gtgagctgcg tccggagagc ggcggcggcg gcaaagaggg acgagacgcc
     5221 ggggaccact tcgtagtcga cccctttatg cgacaggccc tgcatctgct caacgattgc
     5281 cccgtagagc gccggatctc cggaatggag cctcaccacg cgggcgccgc tgcgggcgtg
     5341 caggaccatg gcgtccacga tctcctcaag cttcatcccg tggctgtcaa gtttgatcct
     5401 ggcatgcgac cgctccacca gtcccgggtt cacgagcgat ccggtataga caaggacatc
     5461 ggcctcttcc agcagccgga gccccttgac ggtgatgagg tcggggtcgc ccggtcctgc
     5521 gccgacgaaa cagacctttg ccatctcaca cccccgcgta catgatgctc aggtagtcgc
     5581 aggtctcggg cagttcatcg tcccggtaga tgcgcatgtc cgaaagatac atccgctcga
     5641 cgaggacgaa cctgcgaaag ccctctttcc ggagtctttc tgccgtctcc ttcggccttt
     5701 tgaccttgag gagtatctgg cagcgcggct cacccctgtc cgtgacgacg aacccctcgg
     5761 agagtgatat ccctgcggcg gccgcaaacg cggtgatcgc gctgattccc ggctctgtcc
     5821 tgcaggggat gtccgggtag cgctgcgcca tcacctcgca gaggcgggta aacgtcgaga
     5881 agaagttcgg gtccccgatg agcgcgagga ccgcggtccc cgatgccgca atccctgcga
     5941 tccggtccgc attcctctcc atgcaggaac gtatacggcc ctcgtcccct gtcatcggga
     6001 agtcgaggac ttccgcgtcc ctgtagggag caacgaggtt ctgggcgagc ctgcctggga
     6061 caaagacggc gtctgcctct tgaaggacgc ggacggcccg gagcgtgagg agctccgggt
     6121 cgcccggacc gagcccgagc cctacgagca cccggcggca cccccgacga tgacggcaac
     6181 cgggtgggcc ggtttccaca tgtggccggc cccaagcggg ctcgcatgcg agatctgcac
     6241 caggaccgcc tcgcgaaata tccctaggtc ccgcatgacg gtaatggcgg tattgatggt
     6301 gtcgatcttc accgcgttga cgacgattga gcggacatcc atcccggcaa gcaggtggag
     6361 gatgcggccc atgtcgcggg aacctcccag gaatgcggcg tcggggtggg ggaggccggg
     6421 gatgaggtcg gccgcattcc cctcgatgaa ctcgatgttt tccacacccg cacgctccgc
     6481 ttcatttcgg gcatattcta tggcttcgct cctgatatcg atggcgcaca cgctccctgc
     6541 ctgccgtgcg gcatggatcg agacctttcc cgtcccgcag ccgatgtcta tgaaccggtc
     6601 gccgggacga aggtcaagct tgaagagcga gactgccatc acctcgtcct tcgtgggtcc
     6661 gccgggaagg ctcatttcgc aatcacctac cagatgcgtc ctcgaggagt attaatgctt
     6721 gtattttttg aaaaatgaag ggtattgaag gccggattgg aatgctgtgg cggacttgca
     6781 gccccctgct gtaaggctac ccagggataa ttgcaagagt tggatggcaa taatgcaaca
     6841 tcgtgaaaaa atcgggagcc ggaagtgtta ttcctgtgtg aaagggtatt ttggcccttc
     6901 cgattcaccg gatcaaccgg attgaaaaat tctttctggc agcggaatag agtctcgagt
     6961 ccgcggccac gagaggaaaa ccgcatcggg ctgccgcggc aagaaaaagg gcatcgtaga
     7021 tcgtgatccg gcaggaacac gccatgtcga acgcttcggc catcaggtcg tgtgccggga
     7081 taacccggca ggtctcccga atgaattcct ggctctcctt caacgccgtt ctcgtatatt
     7141 ctagagagat ttcggattag attgcaggag agacgattaa catggccaac aatggtactg
     7201 cgattcttct cccaattatc tactaacctt gatggaatct tccatatata agatattctt
     7261 tctacgtggg aatatacaat tttgcgaata ttacttttgc gtggatagtt actctctgaa
     7321 ggatattttt atgccgacat caaccgcttc tccatgtcga taattgttcc aatccagttt
     7381 ctgatcggac aggcttaccc gataatgcga attgaccggt caaatatctt tatacacgtt
     7441 tttccgatgt ccgatcttca ccacgcagat gatcagtttc tttgtggaga gtgcacagag
     7501 aacgcgatag tcacctgtcc tgaatgagta gagaggagag tctttcaatt tccggagatg
     7561 gagaagcggt ttgtctgcca atccttcgag cttttcgaga acacgcttcc tggtcgcctc
     7621 aggtaaatcg cgaatctggt gaaatgcccg tggggaataa atcaacgtcc atggcatgtc
     7681 aggagaggcc cagttcgcgt ttcacctggt cgtgtgtgta gtagtgacct ttccgtatct
     7741 cggtgagagc ctcgtcgatc tcgttcctgt cctcgtccgt gagaggttct tcgtcgatcc
     7801 tcgattcggc aagccttcgt atgatcgaat cgtaggactc cttcggatag ctccggagga
     7861 gatcgagttt ctctttcgtg gttttatcaa gtcgaacaga ggaagcctgc atatacgggt
     7921 gtatgcatgt gtacacataa tactatggat ttctgaaccg ttccgatttt tggtaaaacg
     7981 gcactcatca tacgacgagg ctcttggggc tccgccccgc cactggggca tccccccaag
     8041 gcgatagggt ccgtgctgcg gatctgaacg taaagccggt tatactccct gcacactggt
     8101 cgcagcctat cgcaggaagg ggaggtccgg ggggattcgc gccctcctgt caatagtaaa
     8161 acagcaatcc ttctctcatg cgcctggacc tttcgtcccg cagaagcgca gcctcaataa
     8221 gacaggtccc agtgcgagag aatcgctttc atcgagcccg cccatatcca tcctctctcg
     8281 cacgcagggt atcttgttgc actattcact tgcggctcgg atattcctca cctaaaaaag
     8341 ctaacctgtc tcctcacagc gggacaagcc ccggatcgtc ttccctgtag agccggacca
     8401 cgtccccaat gacgatgatc gccgggggcc tcacattccg cgactttgca atgtccgcga
     8461 tcgtccggag gttgccgacg gtcacccgct gatcggagcg aaggccccgc tcgatgaccg
     8521 caacaggtgt atcggggtcc ttgccattat cgacgaggct tcgcgtgatg tcggggatat
     8581 tcttcacacc catcaggatg acaagcgtgc ccgggttcct ggcgaggttc tcccagtcga
     8641 ggccggactc cgtcttcccc ggctcctcgt ggccggtgag gatggtcacc tgggaggcat
     8701 atttccggtg ggtaagcggg atccccaccg cgcctgggac cgcgagggcg ctcgtgaccc
     8761 cggagacgag ctctaccggg accccgtgct ccctcatcac ctcgagctcc tcgccgcccc
     8821 tcccgaacag gaaggggtct ccgcc
//
LOCUS       DAOQYR010000088         8435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_8892_length_8435_cov_7.882936, whole genome shotgun sequence.
ACCESSION   DAOQYR010000088 DAOQYR010000000
VERSION     DAOQYR010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8892_length_8435_cov_7.882936"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PKM87_09475"
     CDS             <1..365
                     /locus_tag="PKM87_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30336.1"
                     /translation="DGEFLGVVSTTFSPRALIAPAAGVAMAYAPFIFNVAQEDGKILY
                     HPDPALVGKPTFNETTFARFPGILDLARRYSTERSGYATFSFYRTGSDVVVDKETYWD
                     TVSLHGTEWRVMVIAERG"
     gene            421..936
                     /locus_tag="PKM87_09480"
     CDS             421..936
                     /locus_tag="PKM87_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30337.1"
                     /translation="MKPFHECMDEYRIQMKKGVIREAYRALMEYLMGLRTHFQKEFPE
                     YTVSGNLYPGYMDMTYFSVVPDTFRRRKLKVAIVFLHETCSFEAWLAGSNKQVQAHYW
                     MMFQESGWNRYRMPETIRGADSILEYTLAERPDFYDLISLTAQIESGTREFIRDIEDF
                     LECAEGHNTPR"
     gene            1047..1649
                     /locus_tag="PKM87_09485"
     CDS             1047..1649
                     /locus_tag="PKM87_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30338.1"
                     /translation="MNPLRILLLLGFLALILLPVSAGHPIENVTIVPDKESYSAGTPL
                     SLTTTVRIIPPGPTTTFVEWDELVLGTELENPHWDVVAIAGQQRVEVPHAEGQVVRVP
                     GYLLSYSVDRQVSVSISLEGTVPYTTEPRPFVVLSVDEQNDRGVSVYGPADTISRIAF
                     PSAQKQPVSTLQPSANVALSPLFILVALAGGLVILWWNKR"
     gene            complement(1723..2280)
                     /locus_tag="PKM87_09490"
     CDS             complement(1723..2280)
                     /locus_tag="PKM87_09490"
                     /inference="COORDINATES: protein motif:HMM:NF033823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HNQ30339.1"
                     /translation="MAECQGTTLIPIGRVDPIILAWLKEDLPKGLSITVTITPPLPLT
                     PRHFFKERNQYLADGLLAELTTLNPDGNTLSLGIVDADIFSSGLNFVFGIATTGRALI
                     STFRLRPETYGMPPNPGLYRRRVLTEAVHELGHSLGLPHCEHPGCVMFFSNSIADTDR
                     KGPEFCFRCRRRIEWMGKLREGQPD"
     gene            complement(2554..3351)
                     /locus_tag="PKM87_09495"
     CDS             complement(2554..3351)
                     /locus_tag="PKM87_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991353.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HNQ30340.1"
                     /translation="MKTLIYYFTGTGNSLAVAKGLCSRIGDCELIPIASLIKDPGPIV
                     PDADRVGIVCPLYFFGLPAIVAEFSSCLDLSNTDYVFSVITMGGSGGTAALRQLDGIL
                     KEAPARRGLDSGFSIRMPGNYILMYDSLGQEQIGRTIRAADRRMEEIADRVKQGFREK
                     LAWSPIAALIHHLMNPRFIHGVHEADRKFTVDDRCTSCNTCVEVCPVENIRLENGGPV
                     WLHRCEQCMACIQLCPTNAIQAKKTEGRRRYRHPEIGIGMLKEHARR"
     gene            3546..4871
                     /locus_tag="PKM87_09500"
     CDS             3546..4871
                     /locus_tag="PKM87_09500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ30341.1"
                     /translation="MDDDTTVLELGKLYLEKSGELVVDTARSGYVAKTMLLTHTYDAI
                     ISDYNMPECNGIEFLRHVRKTDQRTPFLFFSDHGEEDVIIDALTSGADFFLPKGISVR
                     SQFIQLEHAIRESVMRRRAEREQRKISSVLRTREAAVRSTLCPLALCDTDGRIQYANP
                     AGLAAWGYADESEVVGRHVSDFVVSPGVSPEDILDLIEQKTWIGQATALRKDGTTFDA
                     RVSVSTMTDEEGDPVGFIASFTDLSRQRRVRMELESYVGDIRFVSEKSSEFADLPPDA
                     DAFLFIADIVSTLSPEGAVVLISSMHNDRIVRVEAVRGPSQVLAAIEGIFGRPVVGIE
                     LRPAGEDFSAMLPKAFIEIDGGIDAITFGQLPPGILEKIDELPFGKVIGTGLSWGGKV
                     NGITVIILPAGVSPGNLDVLDLFVRHCSAVLHRRQLEKLLKGTANLPAK"
     gene            4931..5779
                     /locus_tag="PKM87_09505"
     CDS             4931..5779
                     /locus_tag="PKM87_09505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ30342.1"
                     /translation="MEHKIGDVPATSFVTLYCHAIESRSEHPILHDPKAEEITAELNR
                     TLSRSEDPLDRRLASGRIDRNLVIHIAIRAKKYDEYARDFLAMHPGGVIVNIGCGLDS
                     RFLRIDEGTVFFYDLDLPEIIDLKRRFFRETERYHFIASSVLDFRWMRMLLGLEGPFL
                     FMAEGVFMYLEGERVRSLVLQLRETFPGSELICEVVNSFWLKKPLKTLIDWKMHERGH
                     LGREAVFQSGIRDGREMEQWHPGIRFLGEWSYFDSDEEKLGWLRVFRYIGWVRKTQWT
                     VRYFLG"
     gene            complement(6162..6857)
                     /locus_tag="PKM87_09510"
     CDS             complement(6162..6857)
                     /locus_tag="PKM87_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107644.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNQ30343.1"
                     /translation="MAVLLLLAVLAIGSMSAADTENDNTIVASGTGSVIGTPDRAQIS
                     LAVETENANVQIAQAENAARMKKVMDALTAAGIPTDALKTTGYYIYPVYEDSKLYGQK
                     VRTYRVTNTLTVTLHDVSRVGEVIDIGVAHGINQASSIQFLLSDEQSQVLRTEALKEA
                     SARATADAKTVADALGVSIRSVKSAQIGGGYYPVAFQNYAMADSMSGAASTPIQPGDI
                     TVTATVTVTYWIS"
     gene            7220..7558
                     /locus_tag="PKM87_09515"
     CDS             7220..7558
                     /locus_tag="PKM87_09515"
                     /inference="COORDINATES: protein motif:HMM:NF013089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HNQ30344.1"
                     /translation="MTLNPSLLLVLAIICEVIATSTLKATSGFTRLLPTIAVIAGYLS
                     SFYLLSLVLKEIPIGITYAIWSGVGIVLITLVGYAVYRQALDLPAIAGIALILAGVLV
                     INLWSKSVVH"
     gene            7668..8042
                     /locus_tag="PKM87_09520"
     CDS             7668..8042
                     /locus_tag="PKM87_09520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ30345.1"
                     /translation="MKNQDIGARPPGWQSIEQVFLFAISHLDFLHVTNTLHVKDLEVY
                     ADPLLENILFHMMQNVLLYGEHASEVTLLYKEKAEGLVVVIEDNGVGIPADDKEQIFD
                     QGFGEDRALDCFSSGRSYLSPV"
BASE COUNT         1833 a         2275 c         2422 g         1905 t
ORIGIN      
        1 gtgacgggga gttccttggc gtcgtcagca ccaccttctc accgcgtgca ctgatcgcac
       61 ctgctgcagg cgtggcaatg gcgtatgcgc cattcatctt caacgtcgcc caggaagacg
      121 ggaaaatcct ctatcaccct gacccggccc ttgttgggaa gccgaccttc aacgagacga
      181 cgtttgcccg cttccccggg atactcgatc tcgcacgccg gtactcgacc gaacgctccg
      241 gatatgcgac gttctcattc taccgtaccg ggtcggacgt ggtggtcgac aaggagacct
      301 actgggacac ggtctcgctg catggaaccg aatggagagt gatggtgata gcagagagag
      361 ggtaggtgcg ggaatggctg atctttcata ctgtttgccg gtccaaaagg agatacatcc
      421 atgaaaccat tccacgaatg tatggacgaa taccggatac agatgaaaaa aggggtgata
      481 agggaggcat accgggcact gatggaatac ctcatgggtt tgagaaccca tttccagaaa
      541 gagttcccgg aatacaccgt atccgggaac ctctatcctg gttacatgga tatgacctat
      601 ttctccgtgg tcccggatac gttcaggcgg cgaaaactca aggtcgcgat tgtgttcctc
      661 catgaaacat gcagtttcga ggcctggctg gcaggatcaa ataaacaggt ccaggctcac
      721 tactggatga tgttccagga gagcggatgg aacaggtacc gcatgccgga aacgattcgg
      781 ggtgcagatt ccatcctcga atacacgctg gcggaacgcc ccgattttta cgatctgatt
      841 tcgctgacgg cgcagataga gagtgggaca cgggaattta tcagggatat cgaggacttc
      901 ctggaatgtg cagagggaca taatacaccc cgttgagcgc accaactttt ttcctggatg
      961 agtgtgtctc cggacatggg gaaccgggtc ggggccagta ttatccaccc aatagtattt
     1021 attcccctac atacactatg cccaatatga acccgctccg aatcctcctt ctcctgggct
     1081 ttctcgcgct catcctcctc ccggtatcgg cggggcaccc aatagagaac gtgactatag
     1141 tgccggataa agagtcctat tcggcgggaa cgcccttatc gctcaccacg acagtccgga
     1201 tcatcccccc tggcccgaca acgaccttcg ttgagtggga cgaacttgtc ctcggcaccg
     1261 aactggaaaa cccgcattgg gatgttgtcg ccatcgcggg ccagcaaagg gtggaagttc
     1321 cccatgcgga agggcaggtc gtgagagtgc ccgggtacct cctctcctac agtgttgacc
     1381 ggcaggtgtc cgtcagcatc agccttgagg gaacagtgcc gtatacgacg gaaccacgac
     1441 catttgtcgt tctctcggtt gacgagcaga acgaccgtgg ggtctcggtc tacggccctg
     1501 ccgacacgat ctcccgtata gccttccctt ccgcacagaa acaacctgtt tccacattgc
     1561 agccatctgc aaacgtagcg ctatcgccgc ttttcattct ggtcgcgctg gcagggggac
     1621 tcgtgattct ctggtggaac aagcgctgaa acggggggcc gggtcgggga tggaagaggt
     1681 tacatgtatc tgccgcctgt gtcgttgatg cctgaatgtg ggctaatccg gttgtccctc
     1741 cctcaacttc cccatccact caatccgccg cctgcaccgg aagcagaact caggaccctt
     1801 ccgatcggtg tcagcgatcg agttggagaa gaacatcacg cagccgggat gctcgcagtg
     1861 agggagaccg agcgagtgcc ccagttcgtg gaccgcctca gtgaggaccc gcctgcggta
     1921 cagcccaggg ttcgggggca tcccgtacgt ttcgggccgg agccggaagg ttgagatgag
     1981 cgccctccct gtggtggcaa tgccgaagac gaagttcagg ccggatgaga agatatccgc
     2041 gtccacaatg cccagggaga gggtgttccc atcaggattc agagtggtta actcggcgag
     2101 aaggccgtcc gccaggtatt ggttcctctc tttgaagaaa tgacgaggag tgagcgggag
     2161 gggaggggtt attgtcacag taatggagag gcccttcggg agatcttcct tgagccatgc
     2221 gaggatgatc ggatccactc tcccgattgg gatgagcgtc gtcccctggc attctgccat
     2281 atctggaatg gtgcagggat cggatttatc ctttcaggag tgcgcaaaac aaacggcccg
     2341 cttgaaggtt ctatggtctc tttgtaaaaa ggcatcatga gtatcggggc tcatggggag
     2401 aagccccagg tcggtaccgt caccattttt tagacaattg gtcctggcgg tgcaggctat
     2461 gggtgtcttc ttggcctgca ttgcatcaac aactcttatc ctggcaaggt ttgggtttgt
     2521 gagtgcgaaa ctttcaagaa aaaagagagg gttttacctc ctggcgtgct cctttaacat
     2581 cccgatcccg atttccggat gccggtaccg ccgcctcccc tcggtcttct tcgcctggat
     2641 tgcattcgtg gggcagagct ggatacaggc catgcactgt tcgcagcggt gaagccagac
     2701 agggccgccg ttctcaagcc gaatgttttc tacggggcag acctcgacgc aggtgttgca
     2761 ggaggtgcac cggtcgtcga ccgtgaattt cctatcggcc tcgtgcaccc cgtgtatgaa
     2821 ccgggggttc atcaggtgat ggatgagggc agcgattggt gaccatgcca gcttctctcg
     2881 gaagccctgt ttcaccctgt ccgcgatctc ctccatgcgg cgatcggcag cccggatggt
     2941 cctcccgatt tgctcctgcc cgagggaatc atacatcagg atgtagttcc cgggcatcct
     3001 gatagaaaag cccgaatcaa gaccccttct tgccggcgct tccttcagga ttccgtcgag
     3061 ctggcggagc gccgcagttc cgcccgagcc gcccatggtg atgacagaga agacataatc
     3121 tgtgtttgag aggtcgaggc agctggagaa ctccgcgacg attgcaggga ggccgaagaa
     3181 gtagagaggg cagacgatcc ccacccggtc cgcatccggg acgatcgggc cgggatcctt
     3241 tatcaaagag gcgatgggaa tgagttcgca gtcgccgatc cggctgcaga gcccctttgc
     3301 aaccgcgagc gagttgcccg tgccggtgaa gtaatagata agggtcttca tggtagtccc
     3361 gggaagaatt gtgggagtac tcccggaaaa gagtgacgcc cagatggtcc atttacagcc
     3421 tttctctaaa tgttcacttt cttttgggga tgagtgttaa aggaaattca tataaaaata
     3481 tttaaataaa ccaacagtaa tttttccgta tctctctgag gcattttgtg atccggttac
     3541 tgcacgtgga tgatgacact acagttcttg aactggggaa gctctacctg gaaaagtctg
     3601 gagaacttgt cgtcgacacc gcaagatcgg gatacgttgc caagacgatg ctcctgacac
     3661 acacctacga tgcaattatt tcagattaca acatgccgga gtgcaacgga atcgagtttt
     3721 tacggcatgt ccggaagacc gatcagcgga ccccgttcct cttcttttca gatcacggcg
     3781 aggaggacgt catcatcgac gccctcacca gcggggcaga cttcttcctc cccaagggga
     3841 tcagtgttcg ttcccagttc atccagctcg agcatgcaat ccgggaatcg gtgatgaggc
     3901 ggcgtgccga gcgcgagcag aggaagatct cgtccgtgct gcggaccagg gaggcggctg
     3961 tccggtccac gctctgcccc ctcgcactct gcgacacgga cggaaggatc cagtacgcga
     4021 acccggcggg tctcgcagcc tggggatacg cggacgagag cgaggtggtg ggaaggcacg
     4081 tctccgactt tgtggtatct cccggcgtct cacccgaaga catcctcgac ctgatcgaac
     4141 agaagacatg gatcggccag gcgaccgcac tgcgcaagga cggcacgacc tttgatgccc
     4201 gggtctccgt cagcacgatg accgacgagg aaggagatcc agttggattt atcgcaagct
     4261 tcaccgatct ctcccggcag agacgcgtca ggatggaact tgagtcctac gtcggggaca
     4321 tcaggttcgt gtctgagaaa tcaagcgaat tcgcagacct gccgccggat gctgacgcct
     4381 ttttgttcat tgcagacatc gtgtccactc tctctccgga gggagccgtt gtactcatca
     4441 gttccatgca caacgacagg attgtcaggg tggaagccgt ccgcggtcct tcccaggtac
     4501 ttgccgcgat cgaagggatc ttcggccgcc ctgtcgtcgg tatcgagttg cggccggccg
     4561 gtgaggattt ctctgcaatg ctgccaaaag cctttattga gatcgatgga gggatcgatg
     4621 ccatcacatt cggacagctc ccgccaggaa tcctggagaa aatagatgag cttccctttg
     4681 ggaaagtgat cgggacaggg ctttcctggg ggggcaaggt gaacggcatc acggtgatta
     4741 ttcttccagc cggggtatct ccaggaaacc tcgacgtcct tgacctgttc gttcgccact
     4801 gttcggcggt cctccaccgg aggcagttgg aaaaactgct gaaggggaca gccaatctcc
     4861 ctgcgaaata aaaaatccgc cggaccttta cctggagagc ctgatccgga tataccaagg
     4921 aggaagagag atggagcaca aaatcggcga tgtcccagcc acttcgtttg ttacgctcta
     4981 ttgtcatgcg atagagtccc ggtcggaaca tcccatcctt cacgatccaa aggcggagga
     5041 aattacggcc gaactgaaca ggacactctc ccgatcggag gacccccttg acaggaggct
     5101 cgcctctggc agaatcgacc ggaaccttgt cattcacatc gcgatccggg caaaaaaata
     5161 cgatgaatat gcgagggatt ttttggccat gcacccgggc ggggtgattg tgaatatcgg
     5221 ctgcgggctc gattcccgtt ttctgcgtat tgacgaggga actgtgttct tctatgacct
     5281 tgacctgccc gagattatcg atttgaagag gcggttcttc cgggagacag aacgctatca
     5341 tttcatcgcc tcctccgttc tggacttccg ctggatgcgc atgcttttag gactggaggg
     5401 gccgtttctc ttcatggccg agggagtatt catgtacctg gagggggagc gcgtacgatc
     5461 actggtcctg cagctccggg agacctttcc cggctccgag ctcatctgcg aggtggtgaa
     5521 ctcgttctgg ctgaaaaaac ctctaaaaac actcattgac tggaaaatgc atgaaagggg
     5581 gcaccttggg agggaagcgg tgttccaatc cggcatccgg gatggccgtg agatggaaca
     5641 gtggcacccc gggatcaggt tcctcggaga atggagctac ttcgattctg acgaagagaa
     5701 actgggatgg ctgcgggtat tccggtatat cgggtgggtc cggaagaccc agtggacggt
     5761 tcgttacttt ctgggatgac ctggtgcgat ggcccaatcc cggatgggaa cggcctcaca
     5821 cgggggaagt gggagttatt ccccgttttc atttggcaat ttcccgtcgg atcccctcaa
     5881 gcgatcgctc gcgatctgtc caataatcca ggtgaaataa agccaggatc catgagataa
     5941 cccagaatgc gagcaccagc gcatttgcga gcacgattcc ctggtttata gagttcatga
     6001 ttacacatac cggctgcagg aacgtgctca aggatttcaa ctcgttctgc acggtctact
     6061 acactccccg gcaggcctcc gagtgaggag ctttttcggg gcggatgggg atagttgccg
     6121 gcagtccgcc ctgataaggg catccccctg ctccttcctg tttatgagat ccagtacgtc
     6181 accgtgactg ttgctgtcac cgtgatatca ccgggctgga tgggcgtcga tgctgctccg
     6241 gacatggagt cggccatcgc gtagttctgg aacgcgacag ggtaataccc gccgccgatc
     6301 tgggcggact tcaccgacct gatgctgacg ccgagtgcat cggcaactgt ttttgcatcg
     6361 gccgttgccc tcgcggacgc ctccttcagt gcttcggtcc tgaggacctg ggactgctcg
     6421 tcagagagga ggaactggat ggagcttgcc tggttgatgc catgtgcaac cccgatatcg
     6481 atcacttcgc caaccctcga gacgtcgtgc agcgtgacgg tcagggtatt tgtgacacgg
     6541 taggtcctga ccttctggcc gtagagtttc gaatcctcgt agacggggta gatgtagtac
     6601 ccggtggtct tgagggcgtc tgtagggata ccggcagcgg tcagggcgtc catcaccttt
     6661 ttcatgcggg cggcgttctc ggcctgggcg atttggacat ttgcgttctc ggtctctacc
     6721 gcaagcgata tctgggcacg gtcaggggtc ccgatgacgg accctgtccc ggatgcgacg
     6781 atggtgttgt cgttttccgt atcggctgca gacatgctcc cgatcgcgag gacggcgaga
     6841 aggagcagga ctgccatgaa acgtatgaat atgcggttca ttctcgatca ggaagacgtt
     6901 tcgtgccggc cggtgttaag ggttgctttg aatgcgaacg gattggcagt cattgtggac
     6961 cgtctgccct cctttccgac ctgtcagcct ggagaaatct ctcccctgtg gcgatcaatg
     7021 gcctggttct ccatcacatg accaaggaac accagggatg gcctgtattc cgggcaaatt
     7081 ccgggaccga tacaaacctg tgctgagatg catccggagg ttatggaaga tccaatccgg
     7141 acattatcgc cattatatat cttgaagtcc aataaactca atgcttcggc cggaacgccg
     7201 gcctccccgg taaagaccca tgacactgaa tccctctctc ctcctggtgc ttgccatcat
     7261 ctgcgaagtc atcgccacga gcaccctgaa ggccacgtca gggtttaccc gcctcctccc
     7321 gaccatcgcg gtgattgcag gctatctctc ctctttctac ctgctcagcc tggtgctgaa
     7381 ggagatcccg atcggcatta cctatgcaat ctggtccggc gtcggcatcg tgctcatcac
     7441 acttgtcgga tatgccgtgt accggcaggc cctcgatctc cctgccatcg ctgggatcgc
     7501 gcttatcctc gcgggggtgc tcgtcatcaa cctgtggtcg aagagtgtgg tccattaaat
     7561 cccaaggatg ggggaaacac cagattcccc ggggtcccgt tcatggattg cacccttaaa
     7621 cttcggaaat gatgtgaccg aacggtcccg ttttcttttt ttcgtttttg aagaaccagg
     7681 acataggggc caggccaccc ggatggcaaa gtattgagca ggtcttcctg tttgcaatct
     7741 cccaccttga cttcctgcat gtcaccaata ccctccacgt gaaggacctc gaggtgtatg
     7801 ccgatcccct gctggaaaac atcctgtttc acatgatgca gaatgtgctc ctatacgggg
     7861 agcatgcatc tgaggtaacc ctcctataca aggaaaaagc tgaaggcctt gtcgtggtca
     7921 tcgaagacaa cggcgtgggt attcctgctg atgataaaga gcagatattc gatcaggggt
     7981 tcggggagga tagggccttg gattgtttct cgtccggaag atcctatctc tcaccggtat
     8041 agctttaaaa gagaccgggg agccgggcag gggagcccag ttcgagatcc atgtaccccg
     8101 ggaagaatac cggttcacta aaaacccccc ggtttcctga aaattccttg atatgtgggg
     8161 aaatattcta tcctggcaac ccggatgttg acaaaaagga gtcttcccgg agattcatta
     8221 ctggaatcca ttttttctgg agagacataa gtcctggcca tgggaaggat tggttatcgc
     8281 aaaagagcag ggtgcatccc attgcatcag acccggtgtc tttattcaac caaatgaaac
     8341 atagggaggg ctttcaaaaa agccaaaaaa aaattattcg aaaaggatcc tcaatttgcg
     8401 ttcgataagc ggggagatcc ggtactgccc gggtt
//
LOCUS       DAOQYR010000089         8419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_8920_length_8419_cov_7.948828, whole genome shotgun sequence.
ACCESSION   DAOQYR010000089 DAOQYR010000000
VERSION     DAOQYR010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 8419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8419
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8920_length_8419_cov_7.948828"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PKM87_09525"
     CDS             <1..405
                     /locus_tag="PKM87_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNQ30346.1"
                     /translation="KGGKFRIVFVDHETLEEISRFIGNRIEGPLFLGQMGHHISPRSV
                     QHIFRKYAPQGITPHKIRHSYASELYRRSKNLRVVQENLGHTSIKTTEIYLHTDLDER
                     RKVYQEFFPLAEHPAPAPKPVKKEEEPGAGSQ"
     gene            989..1519
                     /locus_tag="PKM87_09530"
     CDS             989..1519
                     /locus_tag="PKM87_09530"
                     /inference="COORDINATES: protein motif:HMM:NF023043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HNQ30347.1"
                     /translation="MRKLLVFPLLMCLVAAGILFCGCTSEDAAPSASLPPEGSNFTTS
                     TQPTQIPTPDFIVVKKQETVPTLATSTLVIALYSISGWNSSSPLLQPGPGNEYVVVDF
                     SLKNVGAPEGYAYRPDAVKLLDSARQQYTYHDASSSLVNAFRESTIPINETRRGRLVF
                     EVPIAPEGTQYSLMIG"
     gene            1876..2133
                     /locus_tag="PKM87_09535"
     CDS             1876..2133
                     /locus_tag="PKM87_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30348.1"
                     /translation="MEQVINGLKYNTATATLVASSKDGAKHLYRTRNGRFFLHYAHPG
                     QSSVAPYLAAIPLSRAKKEYGSMPRQFVPWEKAFGEEVREA"
     gene            2299..2595
                     /locus_tag="PKM87_09540"
     CDS             2299..2595
                     /locus_tag="PKM87_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30349.1"
                     /translation="MEVTLTSTVYSPETGYDDCYTITYTWTAEDNCGNTETCDQVVTV
                     YDDVKPEFENLPSGSDLGCNPLTLPSCDTGVLATDNCDEMGEVTCTPGPIVEDG"
     gene            2659..3465
                     /locus_tag="PKM87_09545"
     CDS             2659..3465
                     /locus_tag="PKM87_09545"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNQ30350.1"
                     /translation="MKYTWKVDLTPPTVNCPDDAYGEFGDSKHRDNTGYATAEDNCDG
                     VIPVSSYTDGPVTPSTTANCYSYVRNWSAVDECGNIGYCEQVIHVQSSSICGMKYYDA
                     NINGVKDAAEVAVKGVTIQLYNSTGALIATKVTSADGKYCFNHLKLGIYTVKEVLPSG
                     ATWIGTSPTSLSVTLDTYGVVSDGHNFGNICLGEGNGRTLGFWSNENGQALITPAMLT
                     GLNNLPLENAADRDQNFASKTDFRNLLLRGTASSNMGYKLSTQLAAMYLK"
     gene            3831..5726
                     /locus_tag="PKM87_09550"
     CDS             3831..5726
                     /locus_tag="PKM87_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ30351.1"
                     /translation="MESRLTKKITILGLSLALLLLATPAVAANENLYTVEDWSGSDGK
                     DLIQETDLARLSDLNLVDAGGLPFDPLSSPAWHSRFKVWVLRADASSNMAYQLSVQLA
                     AMDLNVNIRNVEGTTSVYAPGTRSVDPLGYATIDALMAEADTELGQHPLTIGGSPDRE
                     YQEALKNALEYANGGTAEAAEGIVVNAPSEIVAEEGELSYTAPGSIKACGDELYGEAT
                     PPKGTDFTAIAAGSYHSLALHSTGAVEAFGWSEYGQTTVPSGTYKAIDPGFIHSIGLL
                     SSGALAGWGDNDNGQISPLPSGTFIAVGAGDAHSIALRSDGTIACWGDNEYGQVSGAP
                     SGTFTAIAAGGSHNLGLRSDGSLASWGYDGYNQVSATPSGNDFVDIAAGPYHSLALRS
                     NGTIVAWGGDFTGQVSGKPSGTNFVAVSAGLEHSLALTSTGTVVGWGSDYAGQYPMPA
                     GTYTAISAGDYYSLGIVGTSITPGAAFSANKTNGIAPMTVQFTDTSLGGDTLTYAWDF
                     NNDGTPDSTAKNPVYTFPAAGTYTIKMRVTSSGGSYDEITKANYIKAQAILAIPGQAN
                     PPTDPDSDGLYEDMNGGGTIGFADVVIFFNNVEWIKANEPVSAFDFSGNGAIGFQDVV
                     IFFNSVG"
     gene            5815..7911
                     /locus_tag="PKM87_09555"
     CDS             5815..7911
                     /locus_tag="PKM87_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ser-Thr-rich GPI-anchored membrane family
                     protein"
                     /protein_id="HNQ30352.1"
                     /translation="MSIEDGQVDAIGGTDTVNIVLDSAPNGLAGYSINITVVNTSVAT
                     ICGISFPSWAYPTNILHSVKITALDPYETVQDGATDVLFATVSIRGLAEGETSVNLAV
                     NLFSDDDDIDITPTISPGIFAVMLPPVANFTANTTSGYAPLTVQFTDLSTEEPTSWSW
                     DFENDGTLDSTVQNPVFTYATPGLYTVKLTATNSKGSDSEVRTNYITVSSGPSLAVTT
                     PHGGEIWTQGSSQTLAWTYSGELGSSVKIGILKGITPLATLASSASIGKGGSGSYLFT
                     FPYYTPLANDYKIRITSNANPAYSDTSDAAFSITSAITVASPNGGETWQPSTTQTLRW
                     DYTDSPGDTVRIEILRSGAALVTLASSAPLGTGGSGSLNFPFPSYTPFGTEYQIKVTS
                     NTYPACTDTSDGYFAFSLLPSITVTAPNGGETWSQNSTQTIQGSYTENPGSTLRIELL
                     RGTTVQQVITAGTSLGSGGSGSFSCAVPASLPLGSDYKIRVTSTSNASYTDTSDAAFT
                     VSEPTSIRVVAPEGGATLYRGDPLVMNWSYTGTPGPTVTIEVFKGPLKMATLPNIPVG
                     SGGNGTFSVKQIPAGTPVGADYTVTVTSTLSPTITDTGDAFAISGPTIHVASPNGGDT
                     FPRGGPLAMSRTYEGNPGSTVNIEVYRGEGRMATLQNIPVGPDGAGSYSVPASPPIKY
                     SGRYRLPDQGNKYPLR"
     gene            8200..>8419
                     /locus_tag="PKM87_09560"
     CDS             8200..>8419
                     /locus_tag="PKM87_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30353.1"
                     /translation="MKQLTLVAFVLLTLTCLVAGASANTLSIGDGQVDAIGATASVNI
                     TLDVALTGLSGYNISITVGDPTIATIESV"
BASE COUNT         2022 a         2331 c         2251 g         1815 t
ORIGIN      
        1 aaggggggca agttccggat cgtcttcgtc gaccacgaga cgctcgagga gatctcccgg
       61 tttatcggca accggatcga ggggccgctc tttttgggac agatggggca ccacatctcc
      121 ccccgctcgg tccagcacat attcaggaag tatgcccccc aggggatcac cccccataag
      181 atccggcaca gctacgcaag cgagctctac cgccggtcca agaacctccg ggtggtccag
      241 gagaacctcg ggcacacctc gatcaagacc accgagatct acctccacac cgacctcgac
      301 gagcggcgaa aggtctacca ggagttcttc ccgctcgccg agcaccctgc ccctgccccg
      361 aaaccggtca aaaaagagga ggagccggga gcggggtctc agtaggaggt gaatcctggg
      421 aaaggcaggg gggaggatcg ctggagcatg ccctgctata gaatgctgag gataccggaa
      481 tgcccctccc tgagaggtac ctaagcgggg taccggtctc tcctctctgg aacagaaatg
      541 ctcaggaaca ggattttatt gagaccgaac cagctcatgt cttcggaagc agatctcttc
      601 acattctgca cgaaattgaa aaatgttttc atatatcgct cgcttccttt ctctctcccg
      661 tcgggctctt ctaacaaata aaccgcataa tgctatacac aaagatgaat ctttttttcc
      721 tcactgcagc attttgcata ttccgatcgc gctcatatgc gttcgtatcc acttcctcag
      781 ataaccttga ctgaatggaa tggtttgttt caaaatctct tgataaacgt tattatgtca
      841 aaataagcgc aaattgctct ttgaatctgg cggccaccac tgcttcggag caggaattca
      901 tgaccggatg gtgcgtgggg atctctctct gggacacccg gcgttccggc agtgagctat
      961 ttattgttgg atactaacct cgtacaccat gcggaaactg ctggtcttcc cccttctcat
     1021 gtgccttgtc gctgcaggga tcctcttttg tggctgcact tctgaagatg ctgccccttc
     1081 tgcctccctc cccccggaag gatcgaattt caccacttcc acccagccga cccagatacc
     1141 caccccggat tttattgtgg tgaaaaaaca ggagacggta cctacccttg cgacctccac
     1201 gctggtaatt gctttataca gcatatcagg atggaattcc agcagtcctc tcctgcaacc
     1261 tggtccgggc aatgaatatg tggtggtgga cttctcgctc aagaatgtcg gggctccaga
     1321 agggtatgca tatcgcccgg atgcggtcaa gctcctggat tctgcccgcc agcagtatac
     1381 ctatcatgat gcatcctcca gtctcgtgaa tgcgttccgg gagagcacca tcccgatcaa
     1441 cgagacacgc cgggggagac tggtctttga agtccctatt gcccccgagg ggacacagta
     1501 tagtctgatg attgggtaaa gatccaggaa cgctccctcc cttttgttcg agaagcttcc
     1561 atggcaggtt cccgctgcat gagagtgtca ttaggggaaa tcctgtactt tttttgctac
     1621 cccgctgtgt tgtgtgggta cattgaatgg atatcaggag acaatgtgtc actgaactcg
     1681 cgccggggca cccttcggtc atggcggcgt actcgcatga gagagagcaa gggcggaccc
     1741 tccccctctc catacactct ctccggcctc tcaaaagtgg cttcatcgag tcctttcatg
     1801 gtcccctttc ctgttgatcc aaccgcaaat cattatggga tcccacaccg tttctctcac
     1861 cattgaggag aatccatgga gcaggtgatc aacggcctga agtacaatac cgcaacggca
     1921 accctggttg catcatcaaa ggacggggca aagcacctct accggacccg gaacggccgc
     1981 ttcttcctcc attatgcaca tcccggccag agctcggtcg ctccctatct tgctgcaatc
     2041 ccgctctccc gggcaaagaa ggagtacggg tcgatgcccc ggcagttcgt cccctgggag
     2101 aaggcattcg gggaagaggt cagggaagcg taaactggct gcaccaatgc aattccctgg
     2161 aaaaaagaga ttggtttatt ttggggtacc gctggcgagc tcctgtccct ggcctgccat
     2221 ccaggcatcc tcgtctcctg tgatgttgcg ctcccggagg taccgcaacc gactgcgagc
     2281 gacaactgtg atgacgatgt ggaagtgacc ctgacctcga cggtatacag ccccgagacc
     2341 gggtatgacg actgctacac gatcacctac acctggacgg ccgaagacaa ctgtggcaac
     2401 accgagacct gcgaccaggt tgtcacggtc tacgacgatg tgaaacctga gttcgaaaac
     2461 ctgccaagcg gtagtgacct tggttgcaac cctctgacct taccaagctg tgatactggt
     2521 gtattggcta ctgacaactg tgatgaaatg ggtgaagtaa cctgcacacc aggtccaatc
     2581 gttgaggacg gctaataccg gagccaggag ttcacgtaca gtgcgattga cgcctgcaag
     2641 aacgcgaaca gcaccatagt gaaatatact tggaaggtcg acctgacccc gccaaccgtt
     2701 aactgtcccg atgacgctta tggagaattt ggcgattcga aacatcgcga caacactgga
     2761 tatgcaaccg ctgaagacaa ctgtgacggg gtgataccgg ttagcagtta caccgatggt
     2821 ccagtaactc ccagcaccac agccaactgc tactcatacg tgaggaattg gtctgcagta
     2881 gatgaatgtg gaaatatcgg gtactgtgaa caagtaatcc atgtccagtc gagctccatc
     2941 tgcggcatga aatactatga tgctaatatc aatggagtca aggatgcagc agaagtcgcg
     3001 gttaaagggg taacgatcca gctctataac tcaaccgggg cactcatcgc gacaaaggtg
     3061 accagtgccg atgggaagta ttgcttcaac cacctgaaac tcggtatcta caccgtcaag
     3121 gaagtcctgc caagcggtgc cacgtggata ggaacatcac caacgagctt atcggtaacg
     3181 cttgacacct acggtgtcgt cagcgatggc cataacttcg gaaatatctg cctaggtgaa
     3241 ggaaatggcc gtacgcttgg attctggtcc aatgagaacg gacaggccct gattacaccc
     3301 gcaatgctta ccggacttaa taatttacca ttggaaaatg cagctgatag agatcagaac
     3361 tttgcaagca agacagattt caggaatttg ctgcttcgcg gaacagcgtc ctccaatatg
     3421 gggtacaaac tctcgaccca gctggcagcg atgtatctga agtgaatgct ccgaccggca
     3481 agggcaggcc aaatgcggtt gatcctaacg catatatcta tgcacctggc accaagagtg
     3541 caaacactct cggattcgca acgatccagc agatcatgaa tgaggctata gatgccttga
     3601 atgagtatcc ggttaagccg acccgtgcat acgaagaaac attaagtagc gctttagaca
     3661 gggcgaacaa taatctgaac tttgttcaga gtaaaccctg tacctgattt agttggatcg
     3721 tacaaccctg cggtcagaaa aaatcctcct tttttgcggg ggagaacact atataaatca
     3781 ttgaagggat gattgttaat tgctcaattc atttaaatag gagtggaaca atggagtcaa
     3841 gactaacaaa aaaaatcacg attctggggc tctcactcgc ccttctcctt ctggcaacac
     3901 ctgctgtggc agctaatgag aacctgtata ccgtagagga ctggtcggga tcggatggga
     3961 aagacctcat ccaggagact gacctggcac ggctctctga tctcaacctg gtcgatgccg
     4021 gaggacttcc ttttgatccc ctctcgtccc ctgcctggca ttcccggttc aaagtctggg
     4081 tgcttcgtgc cgatgcatca tcaaacatgg cctaccagct ctcggtccag ttggcagcga
     4141 tggacctgaa tgtgaatatc aggaacgttg aaggaaccac ctctgtttat gcaccaggta
     4201 cccggagtgt ggatccgctc ggctatgcta cgatagacgc tcttatggca gaggcagata
     4261 ctgagcttgg gcagcatccc ttgaccatag ggggaagtcc agaccgggag taccaggaag
     4321 ccctgaagaa tgctctcgag tatgcgaatg ggggcacagc tgaagcggca gaaggtatag
     4381 tagtgaatgc accctccgaa atcgtggctg aagaggggga gttgagctac accgcaccgg
     4441 ggagcatcaa ggcttgtggg gatgagctgt atggtgaggc cacaccccca aaaggcacgg
     4501 actttacagc catcgcggcc gggtcatatc acagccttgc attgcattcc actggtgcag
     4561 tcgaagcctt tggctggagt gaatacggcc agacgaccgt accatcggga acgtacaagg
     4621 caatcgatcc cggatttatc catagcatag ggctgctctc cagcggtgcg ctggcaggat
     4681 ggggtgacaa tgataacggc cagatctctc cactgccttc gggcacgttc atcgctgtcg
     4741 gagcagggga tgcacacagt atcgcattgc ggtctgacgg caccattgcc tgctggggag
     4801 ataatgagta tggccaggta tcaggtgccc cgagtggaac atttacggcc atcgctgccg
     4861 ggggatccca caaccttggg ttaaggtccg atggctcgct tgccagctgg ggctacgatg
     4921 ggtacaacca ggtttctgcc accccttccg gcaatgattt tgtcgacatc gctgcaggcc
     4981 cctatcacag ccttgccttg cgatcgaacg gcacaattgt cgcctggggt ggagacttta
     5041 ccggacaggt atcggggaaa ccttcaggga ccaactttgt cgccgtatcg gcaggactgg
     5101 agcacagcct tgcactcacc tccactggga cggtcgtagg ctggggctct gactacgcag
     5161 gccagtatcc catgccggct ggaacctata ctgccatctc agccggagac tattacagcc
     5221 tgggaatagt tgggacttca attacaccgg gagcagcgtt cagcgccaat aaaacaaacg
     5281 gcatcgctcc catgaccgtc cagttcacgg acacgtccct tggaggagac acgcttacct
     5341 acgcttggga cttcaacaat gacgggacac ccgacagcac cgcgaagaac ccggtataca
     5401 ccttcccggc agcagggact tataccatca aaatgcgagt aaccagctcg ggcggttctt
     5461 acgacgagat caccaaggcg aattacatca aggcccaggc aatcctggcg atacccggcc
     5521 aggctaatcc tccgaccgat ccagattctg acgggctgta tgaggatatg aatggtggag
     5581 ggaccattgg atttgccgat gtggtcatat tcttcaacaa tgtcgaatgg atcaaggcga
     5641 acgaaccggt ctcggcattt gacttcagcg ggaatggagc gataggcttc caggacgttg
     5701 tcatcttctt caactcggtg gggtgagtat gggcatcggt tctgaaataa gacttttttt
     5761 gcatttttcg ttttctctct ggtatttctc gtgacgggag cctccggaag tgtcatgagt
     5821 atcgaggatg gccaggtcga tgcaattggt ggcacggata ccgtgaacat tgtactcgat
     5881 tcagcgccaa atgggctggc cggatacagt atcaacataa ctgttgtaaa cacttcggtc
     5941 gcaacgattt gtggaatttc gttcccgtca tgggcatacc cgacgaacat tctccattct
     6001 gtaaaaatta cggcacttga cccatatgag actgtgcagg atggtgcgac ggatgtcctc
     6061 tttgcaacag taagcatccg ggggctggca gaaggagaga catcagtcaa cctcgctgtg
     6121 aatttattca gcgatgatga cgatattgac ataactccga cgatttcacc tggtatattt
     6181 gcggtgatgc tgccaccggt cgcgaacttc actgcgaaca cgacgtcggg atatgctccg
     6241 ttgacggtgc agttcacgga cctgtctact gaggaaccaa cgagctggtc ctgggacttt
     6301 gagaacgatg ggaccctgga cagcacggtc cagaacccgg tctttaccta cgcgacacca
     6361 ggcctgtata ccgtgaagct gacggcgacg aacagcaagg ggagtgacag cgaggtcagg
     6421 accaattata tcacggtcag ttcaggacca tcccttgcgg taaccactcc ccacggaggg
     6481 gagatctgga cacagggctc cagccaaacc ctcgcgtgga cctattccgg agaactggga
     6541 tcttcggtga agatcgggat cctgaagggg atcacccctc tcgcaacgct cgcctccagt
     6601 gcctcgatcg ggaaaggtgg ctccggatcc tacctgttca cgttcccgta ctataccccg
     6661 cttgcgaatg attataagat ccggatcacc agcaacgcca acccggcgta tagtgatacg
     6721 agcgatgccg ccttctcgat cacatcagcg atcacggtag catctcccaa cggcggcgag
     6781 acctggcagc ccagcaccac ccagaccctt cgctgggact acaccgatag tcccggggac
     6841 acggtcagga tcgagatact caggagcgga gccgcactcg taacgcttgc ttcgagtgcc
     6901 cctcttggga caggtggatc gggatcactc aatttcccgt tcccctccta cactcccttc
     6961 ggcactgaat accagatcaa ggtcaccagc aacacgtacc ctgcctgcac cgatacgagc
     7021 gatggctatt tcgcgttcag cctgctcccc tcaatcacgg tgacagcacc aaacggcggg
     7081 gagacctggt cacagaattc cacccagacc atccaaggga gctacacgga gaatccaggt
     7141 tcgacactac ggattgagct gctcaggggc actaccgtcc agcaggtaat taccgccggg
     7201 acctcccttg gttcgggagg atccggatcg ttcagctgtg ctgtcccggc cagccttccc
     7261 ctgggaagtg actataagat ccgggtcacc agcaccagca atgcctcgta taccgatacg
     7321 agtgatgccg cattcacggt cagcgagcca acgtcaatcc gggtggtagc gcctgaaggg
     7381 ggagctaccc tctaccgggg tgatcccctc gtgatgaact ggtcgtatac cggcaccccg
     7441 ggaccgacgg tgactatcga ggtgttcaaa ggaccactga agatggccac gctcccgaac
     7501 atacccgtcg ggtcgggagg gaatggcaca ttcagtgtga agcagatccc cgccggcaca
     7561 cccgttgggg ctgattacac ggtcacggtc acgagcaccc tctccccgac catcaccgat
     7621 accggcgatg ccttcgcgat ttccggtccc accatccatg tagcgtctcc caacggggga
     7681 gacaccttcc cgagaggagg ccctcttgcc atgagcagga cgtatgaagg caacccgggt
     7741 tccacggtaa atatcgaggt gtatcgagga gaaggcagga tggcaacact ccagaacatc
     7801 cccgtcgggc cggatggagc cggttcgtac agtgtgcctg ccagcccgcc catcaagtac
     7861 tccggcagat accgtctacc ggatcaaggt aataagtacc ctctacgctg attgcagcga
     7921 tatgagtgat ggtaccttca cgatcagttg gggatgatga ggggatcagg aaaaaatgtt
     7981 cacgatcagt accgcgtgat aagaaggaag gaaaaggaag aagtccctgt ggcggggttc
     8041 acccgccact ttttcgatca tcccggaaaa atgataatcc ggtggaccag aacctgtgta
     8101 gtgaatggtc agggaagagc ccctgggacc tcctctcccg ctgcacccca caaggtccct
     8161 tgtgtccaga cagattcata aaaaaggaac aggattggaa tgaaacagct cacgttggtg
     8221 gcattcgtgc tcttgacgct tacctgcctt gtggcagggg catcggcaaa taccctcagt
     8281 atcggagatg gacaggtcga tgcaatcggg gctacggctt ccgtgaatat tactctcgat
     8341 gtggcactga ccgggctctc cgggtataat atcagtatca ccgtcggtga tccgacaatc
     8401 gcaaccatcg agagcgtct
//
LOCUS       DAOQYR010000090         8336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_9026_length_8336_cov_7.573602, whole genome shotgun sequence.
ACCESSION   DAOQYR010000090 DAOQYR010000000
VERSION     DAOQYR010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8336
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9026_length_8336_cov_7.573602"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKM87_09565"
     CDS             <1..242
                     /locus_tag="PKM87_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30354.1"
                     /translation="PTSTFISFSTDATLQAMKDIAPLVEKGIDQYKKKVLRICLECPH
                     YPDNCPPRRCPVYLGVKIPTCSTRNTLLNMIKGYK"
     gene            complement(462..2288)
                     /locus_tag="PKM87_09570"
     CDS             complement(462..2288)
                     /locus_tag="PKM87_09570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ30355.1"
                     /translation="MNLPDQFTGLNGAWNAALIVTTAAALLLNLAGLIFGITIIFPHL
                     LYIPVVIGAYRFPKQGVAIAAGIGGGYLLTILLIRGVDSPLLIEALIRVCVIIFIGWL
                     IAMLSGRLREQEDLYRGLFDHSEAGSILVREKDGRRVVEEVNWNAAHLLGRDISDLKG
                     SPLTTFLAKEVEDALFLRLSSEGRIYAEQAVVTTPDGSEQQVLISIAALPGNRAIITG
                     VDITGRVNAEEALQTANEKLSLLARISSDHLHQTVNEIIETVDEASAKCQDTRAIGLL
                     ERIRGMAWTLARQIFLTETYQDLGASPPEWIPVQGALRQADTSAAGGDLSIRFWAERL
                     EVYADPLFLDVMSHLVENAIRHGSTAKNLNVSYAETPGALELIVEDDGQGIPDEKKET
                     IFEYDAGRHAGLGLFICRQILSVTGMTISETGRLGKGARFVIRIPDGNWRVEGAADDA
                     PPFPVPSPGIPDWGGEFFTRELLSAEFPVANELWVDYHETKGDPKTDRIFAAFAEGRA
                     VSLARCRRHPDGLEVDAVFTPVAHRGHGYANVTVKGLVEACGNELLYMHSVLNLTGFY
                     GKYGFVTISEDELPPTIKERFAFAGGQLEGANVQPMKREPPP"
     gene            complement(2523..3020)
                     /locus_tag="PKM87_09575"
     CDS             complement(2523..3020)
                     /locus_tag="PKM87_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HNQ30356.1"
                     /translation="MSGSISFVQLEVKKPEDVNIILGHSHFIKTVEDLYEAMVGSVPG
                     ARFGIAFCEASADCLVRRDGNDEELCDMAEKNALAIGAGHSFIICMRDCYPINVLNAI
                     KSLQEVCTIYCATANSVDVIVAESPRGRGIMGVIDGSSPRGVETSENARERIDLLRRF
                     GYKRG"
     gene            3184..5325
                     /locus_tag="PKM87_09580"
     CDS             3184..5325
                     /locus_tag="PKM87_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNQ30357.1"
                     /translation="MAGEEEDRRRFRFSVKTKILLVFLALSVSGLLVTGILAFVQIGD
                     VSRYAVESSSALGDRAVEDSTAAMERDARGSLLRLAQDQAYISNIIFDRVSGEIEMLV
                     RYAGEIQADPSRVRPRHFYLQDEEPQDPASTTVLFLSPGVEKDIPVEERNAAGMMTDI
                     FIPLFASDKNLAAVYVGTESGMSFIYPWFTGMDATFDPRLRGWFRQAKETGAMTWSEP
                     YVDLLGHGLMMTCSKPVSNPTRGWFWVIGADVTVETINRNIIGTQVGESGYAMLIDQH
                     GNVITRPGLSSGDMRWDESFVTENLLESGNAGLVAVARDMTAGKEGVARVSFEGGDRF
                     IAFAPVKSVNWSVGVVMPVDEVIAPALSTRQRILGASEETASHITWQQEQLKTVFTAA
                     FLILLVVVIVLSLVFSRYLTRPLEDLKRGSEAVGKGDLDYQVEVHTGDEFEDLAVSFN
                     RMAGDLKEHIQSLRLTTAEKERIQKELEIARGIQQSFLPESAPRIPGIDLEGLNLPAA
                     EVGGDFYDFIPVGSTSWGLVIADVSGKGVPAALFMALSRTLIRASATRNPDPVESIRE
                     ANHSIYLDSKTSMFVTLFYAILDIREKTLTFVNAGHNPPLLVSPGSSGVTLLKARGIA
                     LGVIDDVDLEPVRVALSPGDVVVLYTDGVTEATNERDEEYGVERLSTLVQASKVSSAR
                     EIIDAIVEDVTAFAGSRPQFDDITLMVLKVK"
     gene            complement(5851..6495)
                     /locus_tag="PKM87_09585"
     CDS             complement(5851..6495)
                     /locus_tag="PKM87_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ30358.1"
                     /translation="MNIGLSRILSVIRRILLSFSRNPGQIYLASDLHLNHAKILRHCH
                     RPFSSLRHMNRDLVDRWNLVVGPRDTVYHLGDFSIRGSPDRWIRELNGKKVFIRGNHD
                     RDLPGAMHHATLSYRGYEFYLVHDPRNAPPSWKGWVIHGHTHNKVPEYPFINGEKRTI
                     NVSCECTGYAPLSIDHLLSLDLGSIHRMETIRTPPVRKETSKTSPSPPECTQQR"
     gene            6783..7184
                     /locus_tag="PKM87_09590"
     CDS             6783..7184
                     /locus_tag="PKM87_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30359.1"
                     /translation="MTGTSRTIRLRDELRDTLCRMMENQGYTSFEEFVNDNLAACIAC
                     RTRHDQTPAGHTYEVEMLRMRIRELERIVKKKDEQITVLLELWEHCNVGGEPAPGLEI
                     SPELEDRIGMIIRDWYYDVERKDHSGADGKT"
     gene            complement(7373..7564)
                     /locus_tag="PKM87_09595"
     CDS             complement(7373..7564)
                     /locus_tag="PKM87_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase family protein"
                     /protein_id="HNQ30360.1"
                     /translation="MPVVTITMAKGRSVEQKKQMAEEIAGTITRVLEVPIERVTILIH
                     ELEKENIAIAGTLLSEPKK"
     gene            complement(7639..>8336)
                     /locus_tag="PKM87_09600"
     CDS             complement(7639..>8336)
                     /locus_tag="PKM87_09600"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HNQ30361.1"
                     /translation="AALLNACRATKKQFEDLHIVVCGAGAAGYAITRLLRCIGYSPDV
                     CRSVREIVVCDTQGIIHRARKGLYSNKYKFILADETNRSGRTGSLSDAMEGADVFIGV
                     SGPGVVNEEMIRLMNDEPIVFAMANPVPEIMPDAARRAGAAVVGTGRSDYPNQINNAL
                     AFPGVFRGALDAFATRISDEMKVAAAHAIADYVPHPHREHILPTILDRDVTRAIARAV
                     KEAAIQSGYARDL"
BASE COUNT         1729 a         2447 c         2401 g         1759 t
ORIGIN      
        1 ccccgacatc tacctttatt tcgttctcca cagatgctac cctgcaggca atgaaggata
       61 tcgccccgct cgttgaaaaa ggcatagacc agtacaaaaa gaaggtgctg cgcatctgtc
      121 tcgaatgccc gcactacccc gacaactgcc cgccacggcg ctgccccgtg taccttgggg
      181 taaaaatccc cacctgttcc acgaggaaca cgcttctgaa catgatcaag ggatacaagt
      241 aggctcatgt cagcgataca actctacttg tcgtgcgttt attccccaca gtgggtgaaa
      301 gagattcggt tgtccaggga acactttcag ccaggagatg tactgataag atccatgtac
      361 tcccgccggc ttctccctct cgcaaaacag gcaggaatta aagagggcct gcaacgatcc
      421 aggggtaagc tttcttcctc atctattgca taggatctct ctcacggtgg cggctcccgt
      481 ttcatgggct ggacatttgc tccttccagc tggccgcctg caaaggcaaa ccgttccttt
      541 atcgtcggag ggagttcgtc ctcactgatg gtgacaaatc cgtatttccc gtagaacccg
      601 gtcaggttca ggaccgagtg catgtagaga agttcgttgc cgcaggcctc cacaagtccc
      661 ttcacagtca cattggcata cccatggccc cggtgggcaa cgggggtgaa taccgcgtcc
      721 acctcgagcc catcgggatg ccgccggcac ctggcaaggg agactgccct cccttctgca
      781 aatgcagcga aaatccggtc ggtctttgga tcgccctttg tctcgtgata atccacccac
      841 agttcatttg caaccgggaa ctcggccgag aggagttccc ttgtaaagaa ctctccaccc
      901 caatcaggga tacccgggga aggaactggg aacggaggcg catcatcggc tgcaccctcg
      961 actcgccagt ttccgtccgg gatacgaatc acgaagcgtg cacccttccc caatctcccg
     1021 gtctctgaga tggtcatccc tgtcacggag aggatctgcc ggcagatgaa gaggccgaga
     1081 cccgcgtgcc ggccggcgtc gtactcgaat atcgtctctt tcttctcgtc tggtatcccc
     1141 tggccgtcat cctcgacgat cagttcaagg gcgccgggtg tctcggcgta ggagacgttc
     1201 agatttttgg ccgttgatcc gtggcggatg gcgttttcca caagatggga catgacgtcg
     1261 aggaagagag ggtctgcgta cacctcgagg cgttctgccc agaaccgtat cgagagatcc
     1321 cctccggcag cagaggtgtc tgcctgcctg agtgccccct ggaccggaat ccattccggt
     1381 ggcgacgccc cgaggtcctg gtaggtctcg gtgaggaaga tctggcgtgc gagggtccag
     1441 gccatccccc ggatcctctc aagcaaaccg attgcccttg tatcctggca cttcgccgac
     1501 gcctcgtcca ctgtctcgat gatctcgttt acggtctggt ggaggtgatc cgaggatatc
     1561 cgggcaagga gggagagctt ctcattcgcg gtctggaggg cttcctcggc gttcaccctg
     1621 ccagtgatgt ccacccctgt gatgatggca cggttgcccg ggagcgctgc aatggagatg
     1681 aggacctgct gctcgctccc atcgggagtc gtcaccactg cctgctccgc gtagatcctc
     1741 ccttcgctcg acagtcgcaa aaagagggca tcctctactt cctttgcaag gaaagtcgtc
     1801 aggggagacc ccttcaggtc cgatatatcc cttcccagga gatgggcggc gttccagttc
     1861 acctcttcga cgacccttct tccatccttc tcccggacaa ggatactccc agcctcggag
     1921 tgatcgaaga ggccacgata caggtcctcc tgctcacgca gccttcccga aagcatggcg
     1981 atcagccatc caatgaagat tatcacacat accctgatca gcgcctcgat gaggaggggg
     2041 ctatccaccc ccctgatcag caggatggtg aggaggtagc ctcccccgat tcccgccgcg
     2101 atcgcgactc cctgctttgg aaaacggtag gctccaatca ccaccgggat gtagaggagg
     2161 tgagggaaga tgatggtgat gccgaagatg aggccggcga ggttgaggag aagagcggcg
     2221 gcagtggtga cgatgagcgc cgcattccag gcaccgttta ggccagtgaa ctggtccgga
     2281 aggttcatgt gcgagtcccc cggcctgcgt cttcgtgctc gatacgactg ttctcttcac
     2341 cccatgcaaa aaaacctccc ccgtacccgg ccctaccccg gaaacccatt cctgccatgg
     2401 taaaagagag acgggggcgg gagtatttgt agctgttcat ttttcctttc gcacacaggg
     2461 aaaacagaac aaggctcacc tgcggatccc aaaaaaaatg gctgtcagga cgcgggagga
     2521 actcatcccc tcttgtatcc gaaccttcgg agaagatcga ttctttcccg cgcgttctcg
     2581 gaggtctcaa cgcccctggg cgatgagccg tcgatgactc ccatgatccc cctcccacgc
     2641 gggctctccg ccacgatcac gtccacagag tttgctgtgg cgcagtatat tgtgcagacc
     2701 tcctgtagtg acttgattgc gttcaggaca ttgatgggat agcagtcccg catgcatatg
     2761 atgaatgaat gccccgcccc aattgccagt gcattcttct ccgccatgtc gcagagctct
     2821 tcgtcgttcc cgtcgcgtcg aacgaggcag tctgcggatg cctcgcagaa cgcgatgccg
     2881 aacctggcac cggggacgga tcccaccatt gcctcgtaca ggtcctcgac tgtcttgatg
     2941 aaatgggaat gaccgaggat gatgttcacg tcttctggtt tcttaacctc taattggaca
     3001 aacgagatac ttcctgacat ctctctcacg gtggagtgat gggaatccct ggatgaaaag
     3061 tattgccggc atttcgcact tcttctgacc cgggcatacc ggattggagc attctcgcgg
     3121 aaaagggcgg ggcagctgtt caaaccgtct ggcttattcc ctcccggaac agacgtgatc
     3181 cttatggccg gcgaggaaga ggacaggagg cggttccggt tctcagtgaa gaccaagatc
     3241 ctccttgtat tcctggccct ctctgtaagc ggactgcttg tcacaggtat cctcgcattt
     3301 gtccagatcg gtgacgtgag ccggtatgct gtggagagca gcagcgccct tggtgaccgg
     3361 gccgtcgagg acagcactgc cgccatggag cgggatgcac ggggatccct gctccggctg
     3421 gcacaggacc aggcatatat cagcaacatc atcttcgacc gcgtttcagg ggagatcgag
     3481 atgctggtgc gttatgccgg ggagatccag gcagatcctt cccgggtgag gccccggcat
     3541 ttctacctcc aggacgagga gccacaagac cccgcctcga ccaccgtcct cttcctctcg
     3601 ccgggagtcg agaaggatat ccccgtcgaa gagcgcaacg ccgccgggat gatgaccgat
     3661 atcttcatcc cactctttgc atccgataag aaccttgcgg cagtctacgt ggggactgag
     3721 agcggaatgt cattcatcta cccatggttc accgggatgg atgccacatt cgatccccgg
     3781 ctgaggggat ggttccgcca ggcaaaggag accggggcca tgacctggtc cgaaccatac
     3841 gttgacctgc tgggccacgg gttgatgatg acctgctcaa aacccgtctc caaccccacg
     3901 agagggtggt tctgggtgat tggggcggac gtgaccgtcg agaccatcaa caggaatatc
     3961 atcggtaccc aggtggggga atccggttat gcaatgctta tcgaccagca cgggaacgtg
     4021 atcacaaggc ccgggctctc atcgggcgac atgcgctggg acgagtcatt tgtgactgaa
     4081 aacctcctcg agagcgggaa tgccgggctc gtggcggttg cgagggatat gactgccggg
     4141 aaggagggcg ttgcacgtgt cagttttgag ggcggcgacc ggttcatcgc gtttgcaccg
     4201 gtcaagagcg tgaactggag cgtgggggtg gtgatgccgg tcgacgaggt gatcgcaccc
     4261 gcgctctcca cccggcagcg catcctcggg gcatcggaag agaccgcgtc gcatatcaca
     4321 tggcagcagg agcagttgaa gacggtattc accgctgcat tcctcatcct tctcgttgtc
     4381 gtgatcgtcc tctccctcgt cttttcccgg tatctcaccc ggcccctcga agacctgaag
     4441 aggggctcag aggccgtcgg gaagggggat ctcgattacc aggtggaggt ccataccggc
     4501 gacgagttcg aggaccttgc agtctcgttc aaccggatgg caggggactt aaaagagcac
     4561 atccagtccc ttcgcctcac taccgcggag aaagaacgga tccagaagga actcgagatc
     4621 gcaaggggga tccagcagag cttcctcccc gagtccgccc cccggatccc gggaatcgat
     4681 ctcgaagggc tcaaccttcc cgctgcggag gtgggcggcg acttctacga cttcatcccc
     4741 gtcgggagca cgagctgggg cctcgtgatc gctgacgtct ccggaaaagg cgtccctgcc
     4801 gcccttttca tggcgctctc ccgcacgctg atccgggcga gcgcgacaag gaaccccgat
     4861 cccgtggaat ccatcaggga ggccaatcac tcgatatacc tggactcaaa gaccagcatg
     4921 ttcgtcaccc tcttctatgc aatcctcgat atccgggaaa agacgttgac gttcgtgaat
     4981 gccgggcaca atccccctct tctcgtatct ccaggaagca gtggagtaac cctcctgaag
     5041 gcaaggggca tcgcgcttgg cgtcatcgat gacgtcgacc tggagccagt cagggtcgca
     5101 ctctcgccag gtgatgtcgt tgtcctctac actgacgggg ttacagaggc aacgaacgaa
     5161 cgtgacgagg agtatggcgt cgagcggctc tccaccctcg tgcaggcgtc aaaggtttcc
     5221 tcggcaaggg agataatcga tgcgatcgtc gaagatgtca ccgcctttgc cgggtcgcga
     5281 ccgcagttcg atgacatcac cctcatggtg ctgaaggtga agtgagggga gagcctcgtt
     5341 gctcctggcc ctccccgtca atccggcatc tcgggggtga tcccgattcc gccgatccag
     5401 cctgcgatca catcgtagac gggtgtggag atcaggccca tgaaaagagg atggtcactc
     5461 ccacgatgat catcgcggag aatcccacga ttcctgctta cgctgcccca agtcctccga
     5521 gccccatggc ggccgcatcg gcactggccc cacgcgcgac cattgcaccc atgaaaacac
     5581 tcatgatgcg tctcgcaaca ggcccggcgc atgaaaatgc cggaggcatt ttggtatgaa
     5641 aaccgcatcc gcggttttca tcaggggggc atgcgactcg acagtctcat gtgcgtttta
     5701 taggaaatcc tccatatcct gctaaatagt cgcgtatacc tttgcaacag acatttcgcc
     5761 gatcttcgat caattacgca actctctctc ccatgcaggg ttccttccaa tcgagggatt
     5821 cccggctttg acgcccggaa agagagaagg tcaccgctgc tgcgtgcatt caggagggga
     5881 gggagacgtt ttagatgtct ccttcctgac cgggggggtg cgaatggtct ccatccggtg
     5941 gatgctccca aggtcgaggg agaggaggtg atcgatggag aggggtgcgt atcccgtgca
     6001 ctcgcacgag acatttatcg tcctcttctc gccgttgatg aagggatact ccgggacctt
     6061 gttatgcgtg tgcccgtgga tcacccaccc cttccacgaa gggggggcat tccgcggatc
     6121 atggacgaga tagaactcgt agcccctgta ggagagggtg gcgtggtgca ttgcgcccgg
     6181 caggtccctg tcgtggttcc cccttatgaa gaccttcttt ccgttcagtt cacgtatcca
     6241 ccggtcgggg ctccccctga tactgaagtc tcccaggtga tacaccgtgt cgcgtggtcc
     6301 caccacgagg ttccaccggt ccacaaggtc ccggttcatg tgacggagcg atgagaacgg
     6361 ccggtggcaa tggcgcagta tctttgcatg gttcaggtgg aggtctgatg caaggtatat
     6421 ctggcccgga ttccgggaaa aagagagaag gatacgcctg attactgaaa gaattctcga
     6481 tagtccgatg ttcattgctc ttgatccgga tctgcatgag tgtagttcaa cagaaaagaa
     6541 gaatgctttc ctgcccaggc gcatcgagtg gcacacaatt taccggggga caggatctgg
     6601 tgctcctgta gcccgggcac gcccgaagta gagcgagggt cacgggtgat accatttgga
     6661 ttcccccggc agccgccgcc gtgcgttccc ggttccatct ttcctgcagt ccttcacggg
     6721 cttttccagg cattccatcc cgtcgaaaca atgaataccc ctccaatgat atgagttccg
     6781 caatgacagg tacatccaga accattcgtc tcagggatga actccgggat actctttgca
     6841 ggatgatgga gaaccaggga tataccagct ttgaagagtt cgtaaacgac aatctcgctg
     6901 cctgcatcgc ctgccgcaca cggcatgatc aaacccctgc aggacacacg tatgaagtcg
     6961 agatgctccg catgagaata cgggagctcg agcggatcgt aaagaaaaag gacgagcaga
     7021 tcacggtcct cctcgaactg tgggagcact gcaacgttgg aggagagccg gcaccgggcc
     7081 ttgaaatatc gcctgaactc gaagacagga tagggatgat catccgggac tggtattatg
     7141 acgtggagcg gaaggatcac tccggtgctg acgggaaaac gtgagggctt tttttcgact
     7201 cttttgaagt ggtgccggga tctgaacagg accgtggcag ggtcaccggg caaccgggga
     7261 aattgcgcaa ccgcggcaat cacgaatgaa ttggacaatc tttaatgcaa ttgttgccat
     7321 gccgataaaa aaaagggatg cataaccggg agggtccggc aggtgcacat ccctattttt
     7381 tcggctcgga gaggagcgtg ccggcaattg cgatgttctc cttctcgagc tcgtggatca
     7441 ggatcgtgac ccgctcgatg ggaacctcga gcacccgggt gatcgtccct gcaatctcct
     7501 ctgccatctg cttcttctgt tcaactgacc gtccttttgc catggtgatg gtgacaaccg
     7561 gcatacctgt acattatggc ggaaacaccg gataaacacg gcggatccgg gacgaagggg
     7621 aacctctttg cctccctttc acaaatccct tgcataccct gactggattg cggcctcctt
     7681 caccgcacgc gcaatcgccc tcgtgacgtc cctgtcaagg atcgtgggga ggatatgctc
     7741 ccggtgggga tgcggcacat agtccgcaat ggcatgtgcc gccgccactt tcatctcgtc
     7801 gctgattcgg gtggcaaaag cgtcgagcgc cccgcggaat acgccgggga atgcaagggc
     7861 attgttgatc tggttggggt aatcgcttcg ccccgtcccc accaccgctg caccggcacg
     7921 ccgggcggca tccggcatga tctctggaac agggtttgcc atggcaaaga cgatcggctc
     7981 atcgttcatc agacggatca tctcctcgtt gaccacgccg ggcccggaga ccccgatgaa
     8041 gacgtctgct ccctccatcg catccgacag cgatcccgtc cgcccggagc ggttggtctc
     8101 gtctgccagg atgaacttgt acttgttgct gtagagtcct ttccttgcgc ggtgtatgat
     8161 cccctgggta tcgcagacta cgatctcgcg gacgctccgg catacgtccg ggctgtaccc
     8221 gatgcagcgg aggagccggg tgatggcata gcctgcagca ccggccccgc agactacgat
     8281 gtggaggtcc tcgaactgct tcttcgtggc ccggcaggcg ttgagaaggg ccgcga
//
LOCUS       DAOQYR010000091         8313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_9064_length_8313_cov_8.639864, whole genome shotgun sequence.
ACCESSION   DAOQYR010000091 DAOQYR010000000
VERSION     DAOQYR010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 8313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8313
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9064_length_8313_cov_8.639864"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1123)
                     /gene="fni"
                     /locus_tag="PKM87_09605"
     CDS             complement(44..1123)
                     /gene="fni"
                     /locus_tag="PKM87_09605"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HNQ30362.1"
                     /translation="MDKTPLTSGRKLDHLRICADEEIERGSPGFEDVRLVHSALPECD
                     LSRIDLSTRFLGQRLNSPLFIAAMTGGHPGTTEVNRCLARAAERYGLGMCVGSQRAAL
                     ENPRLEESFRVVREEAPHAFLSANLGIVQLRDHGLDWAERAVSMIDADAISIHLNFLQ
                     EAIQPEGDHDATGCYDALAALCMESKVPVIVKETGSGISAETARACWGAGVRAIDIGG
                     WGGTSWAAVEAFRAERSPGHQETRLVSLGALFEDWGIPTAVSLCEVASTGSPVIATGG
                     IRSGLDMARALALGADLCGVALPLLKTALESEDTLFTRIETLHRELSTAMFLSGSAKI
                     ADQKRARAYITGKTRQMLEHIQGGK"
     gene            complement(1116..1886)
                     /locus_tag="PKM87_09610"
     CDS             complement(1116..1886)
                     /locus_tag="PKM87_09610"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HNQ30363.1"
                     /translation="MSERLLLKIGGSVLTEKGRQGAADTRAIATIASEIAGHAESSLC
                     LVHGAGSFGHPEAHRYGLAKGADRNNRQGIALTHGAVCRLNQMMVEALRDAGADAIGI
                     HPLGSAWARAGRLLTLETRPLQRLMDVGIIPVLHGDVVMDEVQGACIVSGDQIIRYLG
                     GHLAFTRIGLATDVPGVLTPEGGVINEIPAGVSPEALAGSSGHMDVTGGMAGKVRELQ
                     ELASLGVPSEIFHISRLSDFMDSRPCGGTRVGGGRLRG"
     gene            complement(1879..2748)
                     /gene="mvk"
                     /locus_tag="PKM87_09615"
     CDS             complement(1879..2748)
                     /gene="mvk"
                     /locus_tag="PKM87_09615"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HNQ30364.1"
                     /translation="MATWSAPGKVFLFGEHAVVYGKPGVAMAIRPRVFVTVRKSKSAH
                     HARSPYIDSCFDEMGVKGSVYVNSQLPSSSGLGSSAAVTVATLSAINDEFGKKYSMEE
                     IATMAYAIEKKVQKGKASPTDTSVSTFGGIVMITGTQRRRLPPQNLPLVVGNTLVSHS
                     TAKMVEKVADLKKKNPEICEPILDAIGAVSMCAIRNFSDPHALGNLMNMNHALLEALG
                     AGHPTLSRLVLAARAAGAYGAKLTGAGGGGCMVAICPKHLKSRVVGAVEACDAKAFIT
                     NLDTEGARKEKDV"
     gene            complement(2750..3541)
                     /gene="amrB"
                     /locus_tag="PKM87_09620"
     CDS             complement(2750..3541)
                     /gene="amrB"
                     /locus_tag="PKM87_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNQ30365.1"
                     /translation="MKTRRCGVAGMFYPRDPHHLEQLLEKFFSNKNVGVDALGIVSPH
                     AGYPYSGEVSALAYTAFPASFAGTFVVIGPSHRGYRTCTSLIPWETPLGIVDNDTAFG
                     SSLDLDPDEVSHQEQENSLEVQIPFIKYRFPRARIVPILMGDQTLEGARHLSRTLIDG
                     IRRSGRNDIRIVASSDFSHYVPDAVARENDLYAIAALEHLDVEEFYRRIQSRRVSTCG
                     YGPVATMCLVCRELGASSGRLLRYATSGDVTGDPEVVGYAAIAVM"
     gene            complement(3571..4179)
                     /gene="rpsB"
                     /locus_tag="PKM87_09625"
     CDS             complement(3571..4179)
                     /gene="rpsB"
                     /locus_tag="PKM87_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNQ30366.1"
                     /translation="MTETEMEIELKEPLVPVEEYLAAGVHIGTQQKSKDMMKFIYRVR
                     GDGLYILDIRETDERIKTSAKFLSQFDPTSILVVTSRQYGQYPAKKFAESIGGMAVTG
                     RFIPGMLTNQNLANYIEPQVLVVTDPIGDAQAVKEAVQSGIPILALCDTNNMTSLVDL
                     VIPTNNKGRKALSMIYFLLTREMLRLRGITTSLTPEDFETEL"
     gene            complement(4176..5372)
                     /locus_tag="PKM87_09630"
     CDS             complement(4176..5372)
                     /locus_tag="PKM87_09630"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNQ30367.1"
                     /translation="MTVIRSIQLRTILDSRGNPTVEADIFTDSGFGRSAAPSGASTGL
                     HEAKVKDPGEAVPFAISNLVPGLIGVDSRDQVAFDSILREEDGTSDFSKIGANVAVAL
                     SLANAKAAASSLGMEVYSYLGGVFVPEMPLPLGNIIGGGAHAKGATDIQEFLVVATGA
                     GGPTEAVFANARVHRRVKDILQASGKTCGKGDEGAWAPQIGDHEAFEIICQATGEVSD
                     ELSLSISMGIDVAASQMWDADSGCYVDRNGRRTTEDYIAYIAELVDRYELVYVEDPLY
                     EEDFSGFADLTEQVGDRCLVCGDDLFVTNAARIAEGIEAGSANCALIKPNQVGTLTDT
                     FEAVHLSHLHGMDTVMSHRSGETTDATIAHLATAFGCVFLKTGAVGGERIAKLNELIR
                     IEEQIA"
     gene            complement(5379..5549)
                     /locus_tag="PKM87_09635"
     CDS             complement(5379..5549)
                     /locus_tag="PKM87_09635"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit K"
                     /protein_id="HNQ30368.1"
                     /translation="MKETYTRYERARIIGARALQISMGAPLLIQTSKIDPLEIALEEF
                     QHNVIPITVKRK"
     gene            complement(5581..5655)
                     /locus_tag="PKM87_09640"
     tRNA            complement(5581..5655)
                     /locus_tag="PKM87_09640"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5617..5619),aa:Pro,seq:cgg)
     gene            complement(5656..5844)
                     /locus_tag="PKM87_09645"
     CDS             complement(5656..5844)
                     /locus_tag="PKM87_09645"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit N"
                     /protein_id="HNQ30369.1"
                     /translation="MIPVRCFTCGKVISPAYEEFKRRREAGEDPKKILDDLGMERYCC
                     RRMFLAHKEIIDDLNPYQ"
     gene            complement(5877..6278)
                     /locus_tag="PKM87_09650"
     CDS             complement(5877..6278)
                     /locus_tag="PKM87_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNQ30370.1"
                     /translation="MVKIVNTSGKRKTAIARATVRPGKGRVRINSVPLDFYPNEMVRL
                     KIAEPILLAPKAIEGIDISIDVKGGGMMGQAEAVRTALARGIYRWSNDPQIKDVFLSY
                     DRTLLVNDSRQKESKKPHGRGARKKFQKSYR"
     gene            complement(6293..6715)
                     /locus_tag="PKM87_09655"
     CDS             complement(6293..6715)
                     /locus_tag="PKM87_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNQ30371.1"
                     /translation="MTTIINGEGLLLGRMASIVAKRALNGETIAIVNAEKAIISGSRA
                     RVLSLYGQKRSRGSREGGPFFPRRPDHIVRRTIRGMLPYKRAPGMEAFKRVKVYVGIP
                     IDLVGQEMEVLDEAHVNRLNTPKTVTVGAVSTFLGARY"
     gene            complement(6722..7090)
                     /locus_tag="PKM87_09660"
     CDS             complement(6722..7090)
                     /locus_tag="PKM87_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18e"
                     /protein_id="HNQ30372.1"
                     /translation="MKRRTSKKTNPRLVNLISLLKDTSRQNEVNVWRDVAVRLEAPAR
                     NYAEVNLSKINRYAGDGETIVVPGKVLGSGMLETSVKIAALTFSQSAATKILEANGQC
                     LTIEELVKDNPKGSRIRVLR"
     gene            complement(7071..7916)
                     /locus_tag="PKM87_09665"
     CDS             complement(7071..7916)
                     /locus_tag="PKM87_09665"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit D"
                     /protein_id="HNQ30373.1"
                     /translation="MEIVFSSLDDSSARFRLSGTSQPFANALRRAMIGEVPTLAIEDV
                     RIYDNTSALFDEMLGHRLGLIPIRTDMDCYIPQEQCTCEGAGCDRCSVMFTLSVEGPA
                     MVYSRDLIPQDPRAAPAVDIIPIVKLAKDQKVVLEARAVLNRGRVHAKWQPTTTCGFK
                     NSPVLEISEKCDACGQCVDECPRGILVVRGGKVTVADGRLEECSLCRLCERACLNTGI
                     GEEPAIRVRSEDSRFIFVVEGDGSLPVREILERALFYLKSQSEELCEKIGEVSGVTGD
                     EEENE"
     gene            complement(7916..8311)
                     /locus_tag="PKM87_09670"
     CDS             complement(7916..8311)
                     /locus_tag="PKM87_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNQ30374.1"
                     /translation="MAGEKEKWGIAHIFASFNNTIITVTDLSGAETVCKSSGGMVVKQ
                     DRNESSPYAAMQMASNVAQTAMEKGIVGVHVKVRAPGRGKQRSPGPGAQAAIRALARA
                     GMRIGRIEDVTPVPHDSIRQKGGRRGRRV"
BASE COUNT         1541 a         2651 c         2265 g         1856 t
ORIGIN      
        1 ccgacctcgt cgtacccgcc caccgcgatt atttcaatat ccattatttt cctccttgta
       61 tgtgttccag catctgccgg gtcttcccgg ttatatacgc ccgggcgcgt ttctggtccg
      121 cgatttttgc agaccctgag agaaacattg ctgtgctcag ttccctgtga agtgtctcga
      181 tccgggtgaa gagtgtgtcc tcactctcca gtgccgtttt gagcaggggc agggcgacgc
      241 cgcacagatc cgcaccgagt gccagtgcac gggccatgtc gagcccactt ctgatgcccc
      301 ctgttgcgat cacgggactg ccggtactgg ctacctcgca gaggctcacg gcagtcggaa
      361 tcccccaatc ctcaaacagg gctccaagag agacgagacg ggtctcctga tgcccgggag
      421 atcgctctgc ccggaacgct tctacggccg cccaggaagt tccaccccac cctccgatgt
      481 ctatggcccg gactcctgcc ccccagcatg cccgcgcagt ctcggccgag atgccgcttc
      541 cggtctcctt gacaatcacc gggaccttgc tctccatgca gagcgccgcg agggcatcgt
      601 aacaacccgt ggcatcatgg tcgccttcgg gctggatcgc ctcctgaaga aaattcaggt
      661 gaattgaaat ggcatcggcg tcgatcatcg acaccgcccg ctctgcccaa tccagcccgt
      721 ggtcgcgaag ctgtacaatc cccaggtttg cagagagaaa tgcatgcggt gcctcctccc
      781 gcaccaccct gaagctctcc tcgaggcgcg ggttctcaag cgcggccctc tgggacccga
      841 cgcacatgcc gaggccatac cgttcggcag cccttgcaag gcagcggttt acctctgtgg
      901 tgccggggtg accaccggtc atcgcagcaa tgaagagggg agaattgaga cgctgtccca
      961 gaaaccttgt ggagaggtcg atccgggaaa ggtcacattc cgggagtgca gagtggacaa
     1021 gccgcacgtc ctcgaatccc ggggaaccgc gctcgatctc ttcatctgca cagatacgga
     1081 ggtgatcgag cttcctgccc gatgtaagcg gagtcttatc cacgaagcct gccaccccct
     1141 accctcgtcc cgccgcatgg tcgtgagtcc atgaaatcag atagcctgga gatgtgaaag
     1201 atctcggaag gtactccaag ggacgcaagc tcctggagct ccctgacctt tcctgccatt
     1261 cctcccgtca cgtccatgtg accggagctt ccggccagtg cttccgggga tacccctgcc
     1321 ggtatctcat tgattactcc cccttcagga gtgagcacgc cagggacatc ggtggcaagt
     1381 ccaatcctcg tgaatgcaag atgcccgccc aggtaacgga tgatctggtc gccggatacg
     1441 atgcaggcac cctgcacctc atccattacg acatccccgt gaagcaccgg tatgattccc
     1501 acgtccatca ggcgttgcag aggccgggtc tcaagagtga gcaggcgccc cgccctggcc
     1561 cacgcgctgc cgaggggatg aatgccaatc gcatcagccc ctgcatcccg cagggcctcc
     1621 accatcatct ggttcagccg gcagacagcc ccgtgtgtca gggctatccc ctgccggttg
     1681 ttccggtccg ctcccttcgc cagcccgtac ctgtgggcct cggggtggcc aaatgacccc
     1741 gcgccgtgaa cgaggcagag ggaactctct gcatgtcctg cgatctctga cgcaatcgtc
     1801 gcaatagccc gggtatcggc ggctccctgc ctgcccttct ccgtcaacac gctgcccccg
     1861 atcttgagca gcagtcgttc agacatcctt ctccttcctt gccccttctg tatcgaggtt
     1921 ggtgatgaat gccttggcat cacatgcctc gacagccccc accaccctgc tcttcaggtg
     1981 cttcgggcag atggcaacca tgcaaccgcc tcctcccgca cccgtcagct ttgccccgta
     2041 cgcgcctgct gccctcgcag caaggaccag gcgggagagc gtggggtgtc ctgcaccaag
     2101 ggcctcgagg agtgcatggt tcatgttcat caaattccca agcgcgtggg gatctgaaaa
     2161 attgcggatt gcgcacatcg agacagcccc gattgcatcg agtatcggtt cgcagatctc
     2221 gggatttttc ttcttgaggt ccgcgacctt ctcgaccatc ttcgcggtgc tgtgtgagac
     2281 gagggtgttt cccactacca gggggagatt ctgtggaggc agcctccggc gctgggtgcc
     2341 cgtgatcatt acaatgccac cgaaggtgga gacgctcgtg tcggtggggc ttgcctttcc
     2401 cttctggact ttcttctcga tggcgtacgc catggtcgct atctcctcca tgctgtactt
     2461 cttcccgaat tcgtcgttga tcgccgagag ggttgcaacg gtcacggcag ctgaggaacc
     2521 gaggccagag gatgaaggga gctgggagtt cacgtacacg cttcccttga cccccatctc
     2581 gtcaaagcag ctgtcaatgt agggggacct tgcatgatgg gcggacttgc tcttccgcac
     2641 agtcacaaaa acccttgggc ggatcgccat ggccacgccg ggcttcccat ataccaccgc
     2701 gtgctcccca aacaggaaga ccttgcctgg ggcgctccag gtcgccaccc tacatcaccg
     2761 caatggctgc atacccgacc acttcggggt cacccgtcac atcgccgctg gtggcatacc
     2821 gcaacagccg gccgctggag gctccaagtt ccctgcatac caggcacatg gttgcgaccg
     2881 ggccgtaccc gcatgtgctc accctccggg actggatgcg gcggtagaat tcctccacgt
     2941 ccaggtgctc gagtgcggcg atggcataga ggtcgttctc ccttgccacc gcgtccggga
     3001 cataatggga gaaatcgctc gatgccacga tcctgatatc gttgcgtccg ctcctcctaa
     3061 tcccgtcgat gagtgtcctg gagagatggc gggccccctc gagagtctgg tcacccatca
     3121 ggatgggaac gatccttgcc cgcgggaacc ggtacttgat gaacgggatc tgcacttcaa
     3181 gggagttctc ctgctcctgg tgcgagacct cgtcaggatc gaggtcaagc gaggacccga
     3241 acgccgtatc attgtcgacg attccaagcg gggtctccca ggggatgagc gaggtacagg
     3301 tacgataccc cctgtgactc ggccctatca ccacgaatgt tcctgcaaac gatgcgggaa
     3361 aggcagtata tgcaagggca gacacctcgc cggaatacgg ataccctgca tggggggaga
     3421 cgattccgag cgcatccacc cccacgttct tgttcgagaa gaacttctcc agcagctgct
     3481 cgaggtggtg agggtccctt gggtagaaca tgccggcaac cccgcatctc cgagtcttca
     3541 tcggatctac cctctcgcat ctcacctata ttagagttcg gtctcgaaat cttccggggt
     3601 gagggaagtg gtgatccccc tcaggcggag catctcgcgg gtgagcagga agtagatcat
     3661 cgagagtgcc tttctcccct tgttgttggt aggtatcacg agatccacga ggctggtcat
     3721 gttgttggta tcgcagagcg caaggatggg aatcccgctc tggaccgcct cttttaccgc
     3781 ctgcgcgtca ccgattggat cggtgacaac cagaacctgc ggctcgatgt agttggcaag
     3841 gttctggttg gtaagcatcc ccgggatgaa ccggccggtg accgccatgc ccccgatgga
     3901 ttctgcaaat ttctttgcag gatactgccc gtactgcctt gatgtgacca caaggatact
     3961 ggtcgggtcg aactgggaga ggaattttgc ggaagtcttg atccgctcgt ccgtctccct
     4021 gatgtccaga atatacaatc catctcccct cacgcggtag atgaacttca tcatgtcctt
     4081 gctcttctgc tgggtgccga tatggacgcc cgcggcaagg tactcttcca cgggaaccag
     4141 cggctctttc agctcaattt ccatctctgt ctctgtcatg ctatctgttc ctcgattcgt
     4201 atcagttcat tcagcttggc gatacgctct cctccgactg caccggtctt caggaatacg
     4261 cagccgaatg ccgtcgcaag gtgagcgatg gtagcgtccg tcgtctcccc ggatcggtgg
     4321 ctcatcaccg tgtccattcc atgaaggtgg gagaggtgga ctgcctcgaa ggtatcagtg
     4381 agggtcccaa cctggttcgg cttgatcagt gcgcagttcg cagaccctgc ctctatgcct
     4441 tctgcaatcc gcgcggcgtt ggtgacaaaa aggtcatctc cgcagaccag gcaccggtca
     4501 ccgacctgct cggtgagatc ggcaaaaccc gagaagtcct cctcgtaaag cgggtcctcg
     4561 acgtagacca gttcatacct gtccacaagc tcggcgatgt aggcgatgta gtcctcggtg
     4621 gtacgacggc cgttcctgtc gacgtagcac ccggagtcag cgtcccacat ctggcttgcg
     4681 gcaacatcaa tccccatcga gatcgagaga gagagctcgt ctgacacctc cccggtcgcc
     4741 tggcaaatga tctcaaaggc ctcatggtcg ccgatctgcg gggcccatgc cccttcatcg
     4801 ccttttccac aggtctttcc ggatgcctgg aggatatcct tcaccctcct gtgaacccgg
     4861 gcatttgcaa ataccgcctc agtggggcct cccgcaccgg tggcaaccac caggaattcc
     4921 tggatatcgg tcgcgccctt cgcgtgggca ccgccgccga tgatgttgcc gagcgggagc
     4981 ggcatctcgg gcacgaacac cccgccgaga taggagtata cctccatccc gagggatgat
     5041 gcagccgcct ttgcattcgc aagggacaat gcgaccgcga catttgcccc tatcttcgag
     5101 aaatcggatg ttccgtcctc ctcccgaagg atggaatcga atgcgacctg gtcccgggag
     5161 tcaactccaa taagtcctgg gacgagattt gagattgcga acgggactgc ctctccgggg
     5221 tccttcactt tcgcctcgtg aaggcctgtg ctggctccgc tgggggctgc ggatctcccg
     5281 aaacccgaat cggtgaagat atccgcctcc accgtggggt tgcccctgct gtccagtata
     5341 gtgcgcagtt gaatgctgcg aataaccgtc atggctgact acttcctctt caccgtgata
     5401 ggtatgacgt tgtgctggaa ctcctcaaga gctatctcca gaggatcgat cttgctggtc
     5461 tggatcagca ggggggcacc catcgatatc tgaagtgccc tggctccgat gatcctggcc
     5521 ctctcatacc gagtataagt ctccttcata taccctcaaa atgtatccat gcgcgataaa
     5581 tggggtcgct gagattcgaa ctcaggtcac agcatcccga acgccatagg atggtccagg
     5641 ctaccccacg acccctcatt ggtacggatt gagatcatcg attatctctt tgtgggcaag
     5701 gaacatcctg cggcagcagt agcgctccat gccgaggtcg tcgaggatct tttttggatc
     5761 ctcgccggcc tccctgcggc gtttgaactc ttcatacgca ggtgagatga ctttcccaca
     5821 ggtgaaacat cgtacgggta tcatgccggt atgatctccc agataatttc catatctcaa
     5881 cgataagact tttggaactt ctttctcgca ccccggccat ggggtttctt cgattctttc
     5941 tgccgggaat cgttcacaag gagcgtcctg tcgtacgaga ggaagacgtc cttaatctgg
     6001 gggtcgtttg accaccggta gattccacgt gcaagggccg tgcgcaccgc ttccgcctgc
     6061 cccatcatgc cgccgccctt gacatcgata gagatgtcta tcccctcgat ggccttcggg
     6121 gccaggagga tgggctcggc aatcttcagc cgcaccattt cgtttgggta gaagtcgagc
     6181 ggcactgagt tgatccgcac cctgcccttt cccggccgga ccgtcgccct tgcgattgcg
     6241 gtcttcctct ttccactggt attaacaatc tttaccatgc ctctgattcc tcctagtacc
     6301 tggctccaag gaacgtgctc accgcaccaa ccgtcaccgt ctttggcgtg ttgagccggt
     6361 ttacgtgcgc ctcgtcgagg acttccatct cctggcccac gaggtctatc gggattccca
     6421 catagacctt cacccgctta aacgcctcca tgcctggagc ccgcttgtag ggaagcatcc
     6481 ccctgatcgt ccgcctgaca atgtggtcgg gccttcgcgg gaagaacggt cccccttcgc
     6541 gtgaccccct gctcctcttc tggccgtaga gcgaaagcac cctcgccctg ctcccggata
     6601 tgatggcctt ttcggcattg acaatggcga tggtctcgcc gttcagggcg cgctttgcca
     6661 cgatactggc cattctcccg agaaggaggc cttcgccgtt gataattgtg gtcatctcac
     6721 ctcacctgag taccctgatc ctgcttccct tggggttgtc cttcaccagt tcctcgatcg
     6781 tgaggcactg gccgttcgcc tcgagaatct ttgttgccgc tgattgcgaa aatgtcagcg
     6841 ccgcgatctt caccgatgtc tccagcatcc cgcttccgag aaccttgcct gggaccacga
     6901 tggtctctcc gtcacccgcg tagcggttga tcttgctcag gttgacctcc gcataattgc
     6961 gggccggcgc ttccagcctc accgccacat cccgccatac gttcacctcg ttctgccggg
     7021 aagtatcctt cagcagggaa atcaggttta ccaggcgggg attggtcttc ttactcgttc
     7081 tcctcttcat ctccggtcac tcccgacact tcacctatct tctcacacaa ctcttccgac
     7141 tggctcttca ggtagaaaag cgccctttcc agtatctctc ttacagggag ggagccgtcc
     7201 ccctctacca cgaatatgaa cctcgaatct tcagacctga cacggatggc aggctcttcg
     7261 ccgatgccgg tattcaggca ggcccgctcg cagaggcggc agagcgagca ttcctccagc
     7321 cttccatctg caactgttac ctttccgccc ctgacgacga ggatgcccct tgggcattcg
     7381 tcaacgcact gtccacaggc atcgcacttt tcgctaatct ccagcacagg ggaattcttg
     7441 aacccgcagg tggtggtcgg ctgccacttg gcatgcactc gccccctgtt gagaactgcc
     7501 cttgcttcga gtactacctt ctggtctttt gcgagcttga cgatggggat aatatctacg
     7561 gcaggtgctg cgcgcgggtc ctgtggtatc aagtcccgtg aatacaccat ggcgggcccc
     7621 tcaacggaga gcgtgaacat aacactgcac cggtcacagc ccgccccttc gcaggtgcac
     7681 tgctcctgcg gaatatagca gtccatgtcg gtacggatgg ggatcagccc aaggcgatgc
     7741 ccgagcatct cgtcgaagag tgcgctcgtg ttgtcataga tgcgcacgtc ctcgattgca
     7801 agcgtgggca cttctccgat cattgccctg cgcaacgcgt tggcaaacgg ctggctcgtc
     7861 cccgaaagcc tgaaccttgc cgaagagtca tcaaggcttg agaacactat ctccatcaga
     7921 ctctcctgcc cctccggccg cctttctggc ggatggagtc gtgggggacc ggagtgacgt
     7981 cctctatgcg cccgatgcgc atgccggcgc gggcaagtgc gcggatggct gcctgggcac
     8041 ccggccccgg gctgcgctgc tttccccttc cgggggcacg gaccttcacg tgcacgccta
     8101 cgatcccctt ctccattgcg gtctgggcaa cgttcgatgc catctgcatg gctgcatacg
     8161 gggagctctc gttcctgtcc tgcttgacca ccatgccgcc gctgctcttg cacaccgttt
     8221 cggcccccga gaggtcggtg acggtgatga tggtattatt gaatgacgcg aatatgtgcg
     8281 caattcccca tttttccttc tcgcctgcca ttc
//
LOCUS       DAOQYR010000092         8214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_9201_length_8214_cov_10.036156, whole genome shotgun sequence.
ACCESSION   DAOQYR010000092 DAOQYR010000000
VERSION     DAOQYR010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9201_length_8214_cov_10.036156"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..83)
                     /locus_tag="PKM87_09675"
     CDS             complement(<1..83)
                     /locus_tag="PKM87_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNQ30375.1"
                     /translation="MTIKLGFVVSEFNRDITYMMEVEGREHA"
     gene            complement(142..600)
                     /gene="ribC"
                     /locus_tag="PKM87_09680"
     CDS             complement(142..600)
                     /gene="ribC"
                     /locus_tag="PKM87_09680"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNQ30376.1"
                     /translation="MKIGVADTTFARVNMGGIAVDELKKHASVSIERVTVPGIKDLPV
                     ACKKLIEERKCDIVMALGMPGGKEKDRMCAHEASMGLITCQLMTNTHIVEVFVHEDEA
                     KDDRELAWLAGSRTREHAENAIKMVLHPKELIKEAGTGQRQGFPDAGPAR"
     gene            complement(597..1727)
                     /locus_tag="PKM87_09685"
     CDS             complement(597..1727)
                     /locus_tag="PKM87_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNQ30377.1"
                     /translation="MEKEPLLMLPGPVPMPERVRAAMMRQAINHRGKEFSAVYADCVR
                     ILKTAFGTGNDLFVISGSGTAAMEAAVGNFGRGRKIACLVNGKFGERLYKISQRYGAA
                     QEIGSEWGTPLDLGKLEEALSAGAEMVTLVHNETSAGIRNPAEKIGRMARTHDALLVM
                     DGITSIGGDEVKADAWGADVVVVGSQKCLAAPAGLSAVSVSSRAWERMTEPRPFYLDL
                     AAYRKSASAKLMETPYTPAVPLFMALREACLIMEEEGLLARIARHRRMADAVRAAARA
                     WGLPLFPKIDSDHAYSNTATAITLPPGIEDADFRGTVKKMGIEIAGGQDHLKGKIFRI
                     GSMGAVSAPEVLATLAAVQYALKAQGYAAKDDGVMAAAGVLV"
     gene            1786..2175
                     /gene="purE"
                     /locus_tag="PKM87_09690"
     CDS             1786..2175
                     /gene="purE"
                     /locus_tag="PKM87_09690"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNQ30378.1"
                     /translation="MVDVAILSGSASDAAIVEKAAAVLQAHGLTYDARVISAHREPDL
                     LDEYIRSSDCRVYIAIAGLSAALPGVIASRTKKPVIGVPVSGKVMGGLDAVLSIVQMP
                     KGVPVACVGVDNGENAALLAIRILDLH"
     gene            2273..3652
                     /locus_tag="PKM87_09695"
     CDS             2273..3652
                     /locus_tag="PKM87_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ30379.1"
                     /translation="MTGRREKRTLLFVSSLASFLVPYTVSSLNIALPAISGAFGLDAV
                     TLGWVTSAYMLTAAVCIVPFGKIADIYGRKRVFLVGNLLFVAGSVFAAVSWAGYALVA
                     ARIVQGLGGAMVFSTSIAIITAVFSPGERGRAIGIVTATVYAGLSLGPFLGGILTQHA
                     GWPSIFLLNVPLGVAVIALTLTRIPGEWADLGQREFDLAGSVLYGLMLIGCIYGLTLL
                     PSTSGLIWMSAGLVLLLAFWWWEHRSPHPVLDPALFSKNHVFLYSNLAALINYAMVFA
                     VGFLLSLYLQFNRGLDPQTAGIFLVAQPVVQTLVSPAAGHLSDSIDPRVLATAGMAFS
                     TAGLGILAFLSTDTPLVVILGGLVILGIGYGLFSSPNTNAIMSSVGVHHLGIASGMVS
                     TMRAIGQMVSLAIAMVVFSVVIGTVQISPAVYPELQQSVNLSFSVFFVLGLIGIWASY
                     TRGNTHIKK"
     gene            3775..4119
                     /locus_tag="PKM87_09700"
     CDS             3775..4119
                     /locus_tag="PKM87_09700"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HNQ30380.1"
                     /translation="MNKIETVCGYSCSECSYLWKECTGCGNTRGKPFWTQFAGVDICP
                     IYDCCVNDRMLAHCGKCPELICERFTRFKDPGLTEEETRASLRDMEQELRQRSRRESG
                     HSTHPPKRQGES"
     gene            complement(4150..5433)
                     /gene="thiC"
                     /locus_tag="PKM87_09705"
     CDS             complement(4150..5433)
                     /gene="thiC"
                     /locus_tag="PKM87_09705"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNQ30381.1"
                     /translation="MSVMPSLIRECLTGVPPLVEAVARMEGINPRVVARSVARGRLVI
                     PANPGRSHRPCGIGEGCRVKVNVNVGTSGERCDLSLEKAKARAALENGADTLMDLSTG
                     GDLPAVRRMVLSMDAPVGTVPVYEAVRRAGSAADVDADLLFSVIRDHCRDGVDFLTLH
                     CGVNREALSSLHSDPRLMPVVSRGGAFHVAMMVQRGEENPLYAEFDYLLEILAEYGVV
                     ASLGDGMRPGCLQDADRHAKTVEYLTLGRLATRALDAGVQRMIEGPGHIPLGQVEYNV
                     LVMKELSNDAPLYLLGPLVTDLAPGYDHLVSAIGGAVAAMHGADFLCMVSPSEHLALP
                     DENDIIEGARTAKIAAHVGDISRTREGYKHPREIRMAEARRNLDWESQFSLALFGNHA
                     REIHSRDGDLDTCSMCGDLCAVKMVREVFDGEKEK"
     gene            5463..6065
                     /locus_tag="PKM87_09710"
     CDS             5463..6065
                     /locus_tag="PKM87_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ30382.1"
                     /translation="MPVRWIPGGGFLGNSYVVGGVLVDAGVLPTAIERYRDQIEEIVL
                     THCHYDHIAYVHEIARICDAPVHIHRLDAPGLSSDAISLSMHFGASAPRHLPDRLLEE
                     GDRVGDLLVLHTPGHTPGSICLLKEDEHLLLSGDTVFPDGGFGRFDFPGGSRADLVRS
                     LERLSSLDIEGLFPGHGSPVERGGKRHILAAKELLRITYA"
     gene            6058..6492
                     /locus_tag="PKM87_09715"
     CDS             6058..6492
                     /locus_tag="PKM87_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF123 domain-containing protein"
                     /protein_id="HNQ30383.1"
                     /translation="MHSGVYCLVLENTPCREQVGALGPVEFPGGFHVYVGSALGPGGL
                     SRVARHIRCAREGIARPRWHIDYLLASDAFILRSVFCIPTEERLECRLASLIPGRPVP
                     GFGCSDCDCASHLFSFSEHPRVHLARAASKLGLSAHSKTIKT"
     gene            6511..7062
                     /locus_tag="PKM87_09720"
     CDS             6511..7062
                     /locus_tag="PKM87_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNQ30384.1"
                     /translation="MSVLGISGSMRKDGNTSLLVKAVLERCENAGIKTEFISLAGKDI
                     KPCLGCEKCKEKDWCVIKNDDWGEIMEKVLDCEVLVIGSPTYYFDVCGHLKNFIDRSY
                     SLYHRRKLAGRKAVAVAVCANKGGARTIETLEGFLNSHEFSYLGWVRGKGYLEGEITK
                     DQKGLKRAQEIGDKIVQLLKPHD"
     gene            complement(7217..>8214)
                     /gene="cofH"
                     /locus_tag="PKM87_09725"
     CDS             complement(7217..>8214)
                     /gene="cofH"
                     /locus_tag="PKM87_09725"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HNQ30385.1"
                     /translation="WRVANAADEQRERVVGDAVTYVRNQNINVTNICINSCGFCGFSR
                     KPKDKDAYHFPAEVVREKAALARSRGVTEICTVSGLHPDFDAGSYLEIISCIREGAPG
                     VHIHASNPMEVAYGAGKSGIPTIEMLGLMKEAGLGSLCGTAAEILVDRVREVICPGKV
                     STKDWVRIIREAHSLGIRSTATIMYGHCETVEDQARHLALIRDIQDETGGFTEFVPLS
                     FIHRETPLFRKGLARAGATGREDLLMTAVSRLFLDNIPHIQVSWVKLGLKMAQICLLA
                     GGDDLGGTMFEESISKGAGAVDTDYLDPEEMRRMAGDIGRTLKQRTTLYTDPGHT"
BASE COUNT         1552 a         2611 c         2302 g         1749 t
ORIGIN      
        1 gcgtgctccc gtccttccac ttccatcatg taggtgatgt cccggttgaa ctccgagacc
       61 acgaacccaa gctttattgt catcatgtcc tcctcctgaa taatctcctt cctgcagggt
      121 cataacgata tcccgaactg ttcagcgggc agggcctgca tcaggaaacc cctgcctctg
      181 gcccgtccct gcctccttta tgagctcttt tggatgaagc accattttta tcgcgttttc
      241 tgcgtgctcc cgcgtgcgcg acccggcgag ccaggccagt tcccggtcat cctttgcctc
      301 gtcctcgtga acaaagacct cgacgatgtg cgtgtttgtc atcagctggc aggtgatgag
      361 gcccatcgat gcctcgtggg cgcacatgcg gtctttctct ttgccgcccg gcatcccgag
      421 cgccatcacg atatcacact tcctctcctc gatcagcttc ttgcaagcga cggggaggtc
      481 cttgataccc gggacggtga ctctctcgat gctgacactc gcatgcttct tcaattcatc
      541 aacggcaatg ccccccatgt tcacccgtgc aaacgtggta tcggcaacgc caattttcat
      601 accagcaccc cggcggccgc catcacccca tcgtcctttg cggcataccc ctgtgccttg
      661 agggcatact ggaccgcggc aagagtcgcg agaacctctg gggcgctgac ggcacccatg
      721 ctcccgatcc ggaagatctt ccccttcagg tggtcctgcc cgccggcgat ctcgattccc
      781 attttcttga ccgtgccccg gaagtcggcg tcctcaatcc cgggagggag cgtgattgca
      841 gtggcggtgt tggaatacgc gtgatcggaa tcgatcttcg ggaacagggg cagcccccat
      901 gccctggctg cggcacgaac cgcgtctgcc atgcggcggt gccgtgcaat ccgggctaaa
      961 agcccctctt cctccataat caggcaggcc tcccgtagcg ccatgaagag ggggaccgcg
     1021 ggggtgtagg gggtctccat cagcttcgcc gaggcactct tcctgtacgc ggcgagatcc
     1081 aggtagaacg ggcgcggttc ggtcatacgc tcccacgccc tgctgctgac agacaccgct
     1141 gaaagcccgg cgggtgcggc aaggcacttc tgggagccca cgacaaccac gtccgcaccc
     1201 catgcatcgg ccttgacctc gtcccccccg atcgaagtga tgccgtccat caccaggagc
     1261 gcatcgtgcg tgcgggccat cctccctatc ttctccgccg ggttcctgat ccccgccgag
     1321 gtctcgttgt gcacaagggt gaccatctct gccccggccg agagcgcctc ctcgagtttc
     1381 ccgaggtcga gcggagtccc ccattcggac ccgatctcct gggcagcccc gtaacgctgc
     1441 gatatcttat agagccgttc cccgaactta ccgttcacga ggcaggcaat cttcctcccc
     1501 cggccgaagt tccccaccgc agcttccatg gccgcagttc cggacccgct gatcacgaag
     1561 aggtcgtttc cggtcccaaa cgccgtcttc aggattcgca cgcagtccgc gtataccgcc
     1621 gaaaactcct tcccgcggtg gttgatcgcc tgtcgcatca tcgccgcccg cacccgttct
     1681 ggcatgggta ccgggcctgg cagcatcaga agaggttcct tttccattct caccagccca
     1741 tagtattttt ataacccggg catataagtg tggatggcga atccaatggt cgacgtagcg
     1801 attctcagcg gatcggcctc agatgcggcc attgtggaga aggccgcggc agtcctccag
     1861 gctcacggcc ttacctatga tgccagggtc atctccgccc acagggagcc tgatctcctt
     1921 gacgaatata tacgatcgag cgactgcagg gtctatatcg ccatcgcagg gctctctgct
     1981 gctcttcccg gcgtcatcgc ctcaaggaca aagaagcctg tcatcggtgt cccggtgagc
     2041 ggaaaggtga tggggggact cgatgcggtt ctctccatcg tccagatgcc aaaaggcgtc
     2101 ccggtggctt gcgtcggggt ggataacggg gagaacgccg ccctgctcgc aatccgcatc
     2161 ctggatctcc attgaaagtg ctctttttcc tgcaggcacg catacccgca aaggagtggg
     2221 tccagctaat caggcactaa cctcatctcc tcatgcagcg atctttcctc tcatgacagg
     2281 gagaagggag aagaggaccc tcctgttcgt gtcctctctt gcatccttcc ttgtccctta
     2341 cacggtctct tcgctgaaca ttgcgctgcc cgcgatatcg ggtgcgttcg gactggatgc
     2401 agtcacgctg ggctgggtca cctccgccta catgctcacc gcagccgtct gcattgtccc
     2461 gttcggaaaa atcgccgata tatacgggag gaaacgggtc ttccttgtgg gaaacctgct
     2521 tttcgtggcg gggtctgtct tcgcagcggt ttcctgggca ggatatgcgc tcgttgccgc
     2581 ccgcatcgtc cagggcctcg gcggggccat ggtcttctcc acctcgattg ccatcatcac
     2641 ggctgtcttc tctcccgggg agcgcgggcg ggcaatagga atcgtgaccg caacggtgta
     2701 cgcggggctc tcgctcggac cgttcctcgg cgggatcctg acgcagcatg ccgggtggcc
     2761 gagcatcttc ctcctgaacg ttccgctcgg cgttgcagtt atcgcactga ctctcacgcg
     2821 gatcccggga gaatgggctg acctggggca gagggaattc gacctcgctg gttcggtcct
     2881 gtacgggctg atgctgatcg ggtgtattta cgggctcacg ctccttcctt ccacttcagg
     2941 gctaatctgg atgagtgcag gcctggtgct tctcctcgca ttctggtggt gggaacaccg
     3001 gtcaccccac cccgttctcg accccgcgct cttctcgaag aaccatgtct tcctgtattc
     3061 aaacctcgca gccctgatca actacgcaat ggtctttgcc gtgggtttcc tcctctcgct
     3121 ctatctccag ttcaacaggg gactcgatcc ccagacggcc ggtattttcc tggtggcgca
     3181 gccagtagtc cagaccctgg tatcccctgc cgccggtcac ctctcggact caatcgaccc
     3241 ccgggtcctt gcaactgcag ggatggcttt ctccacagcc gggctgggga ttctcgcatt
     3301 cctgtccact gacacccccc tcgtcgtaat ccttggaggc ctcgtcatcc tcggcatcgg
     3361 gtacggcctc ttctcctcgc cgaacaccaa cgccatcatg agttccgtcg gggttcacca
     3421 cctgggcatc gcatcaggga tggtatcgac catgcgggcc attggccaga tggtcagcct
     3481 tgccatcgcc atggttgtct tctccgtcgt gatcggaaca gtccagatct cccctgccgt
     3541 ctaccccgaa ctccagcaga gcgtgaacct ctcgttctcg gtcttctttg tccttggcct
     3601 catcgggatc tgggcatcct acacccgggg aaacacgcac attaagaaat gattcgaacc
     3661 tgagaaaacg tgtattcgcg cgcaggggat gccggatacc catgttcaca ccctcacttt
     3721 cacactcctc ttacctccct tttttatacc aaagattcac cccttttcat ggtaatgaac
     3781 aagatagaga ccgtatgtgg ttattcatgc agtgaatgca gttatctatg gaaagaatgc
     3841 accggttgcg ggaatacccg gggaaaacca ttctggaccc agtttgccgg cgttgatata
     3901 tgtccaatat acgactgctg tgtgaatgac cgaatgctgg cacactgtgg aaaatgcccg
     3961 gagttgatat gtgaacgttt cacccggttc aaggatcctg gcctgaccga ggaagaaaca
     4021 cgggcatcat tgcgcgatat ggaacaggag ctccgccaaa ggagcaggag agaatccggg
     4081 cacagtactc atcctccaaa aagacagggg gagagttaag aggagatttg ccctgaagag
     4141 atccgaattc tatttttctt tctccccatc gaagacctcc cgcaccatct tcacggcaca
     4201 gagatccccg cacatggagc aggtatccag gtcgccgtcc cttgagtgga tctccctcgc
     4261 atggttacca aagagcgcca gggagaactg tgattcccag tcgagattcc ggcgggcttc
     4321 cgccatcctg atttcacgcg gatgcttgta gccctcccgc gtcctggaga tgtccccgac
     4381 atgtgcggca atctttgccg tccgcgcccc ttcgatgatg tcattttcat cagggagcgc
     4441 aaggtgctcg cttggggaga ccatgcaaag gaaatcggcg ccgtgcatcg ccgcgaccgc
     4501 gccgccgatc gcagagacca ggtggtcata tccgggcgcg agatcggtga ccagggggcc
     4561 gaggaggtag agcggggcgt cgttcgagag ctccttcatc accaggacat tatactccac
     4621 ctgcccgagg ggaatgtgcc cgggcccctc tatcatacgc tggactcctg catcaagagc
     4681 ccgggtggca agccgcccaa gggtcaggta ttcgacggtc tttgcatgtc ggtctgcgtc
     4741 ctgcaggcac cccgggcgca tcccgtcgcc aaggctcgcc accaccccat actctgcgag
     4801 tatctcgagg aggtagtcga attctgcata gaggggattc tcctcccccc gctggaccat
     4861 catggccacg tggaaggcac caccccgcga gaccaccggc atgagccgag gatctgagtg
     4921 aagggaagag agcgcctccc ggttcacccc gcagtggagg gtgaggaagt ccacgccgtc
     4981 acggcaatga tcccgtatca ccgagaagag gaggtccgca tcgacatcag ccgcgctccc
     5041 cgccctccgg accgcctcgt acacggggac ggtcccaacc ggcgcgtcca tcgagaggac
     5101 cattctccgc acggccggga ggtctccccc cgtagagagg tccatcaggg tgtcggcgcc
     5161 gttctccagg gccgcccggg ccttggcctt ctcgagtgac aggtcgcaac gctcccctga
     5221 agtgccgacg ttcacgttga cctttaccct gcacccttcc ccgattccgc acggccgatg
     5281 gcttctcccc gggttggccg gaatcaccag cctccccctc gcaaccgagc gcgcaaccac
     5341 ccgtgggttg attccctcca tcctggcgac agcctccacc aggggaggga ccccggtgag
     5401 gcattcacgg atgaggctag gcatgacaga cattttggtc tgcaaaccta aattagtcta
     5461 ctatgccagt ccggtggata ccaggaggag ggtttcttgg caactcctac gtcgtgggag
     5521 gagtgctcgt ggacgcaggg gtcctcccca ccgcaataga gagatatcgt gaccagattg
     5581 aggagatcgt cctcacccac tgccactacg accacattgc ctatgtgcat gagatcgcca
     5641 gaatctgcga cgcgcccgtc cacatccacc gcctggacgc gcccggcctc tcgagcgacg
     5701 ccatctcgct ctcgatgcac ttcggggcca gcgcgccacg gcatcttccc gaccggctcc
     5761 ttgaggaagg ggacagggta ggggatctcc tcgttctcca taccccgggt cacaccccgg
     5821 gcagcatctg cctcctcaaa gaggacgagc atctcctcct ctcgggggac accgtctttc
     5881 ctgacggtgg cttcgggcgc ttcgatttcc caggggggag cagggcagat ctcgtgcgat
     5941 cgcttgaacg actatcctcc cttgacatcg aggggctctt tcccgggcac ggctcccctg
     6001 tcgagagggg cgggaagcga cacatccttg cagcgaagga attgctgcgc atcacctatg
     6061 catagcggcg tatactgcct ggtcctggag aacactccct gtagagaaca ggtgggtgcg
     6121 ctcggacctg tcgagttccc cgggggattc catgtctacg tcggttccgc gcttgggccc
     6181 ggcgggcttt cgcgggtggc acggcacatc cgctgtgcaa gagaagggat agcgcggccg
     6241 cgctggcata tcgattacct gctcgcatcc gatgcattca tactccggag tgtattctgc
     6301 attcccaccg aagaacgcct ggagtgccgg ctcgcatccc tcataccggg gaggccagtc
     6361 cctggatttg ggtgcagcga ttgcgactgt gcctcccatc tcttctcctt ttcggaacat
     6421 ccgcgtgtgc atcttgccag ggccgcatcg aaacttggcc tttctgctca tagcaaaaca
     6481 atcaaaacgt aggaggtaaa ctgttagtcc atgagcgtgc tgggtatttc ggggagcatg
     6541 cggaaggatg gcaatacctc cctgctggtc aaggctgtgc tggagcgctg cgagaatgcg
     6601 ggcatcaaga ctgaattcat ctcccttgca ggaaaggata tcaaaccatg cctcgggtgc
     6661 gagaagtgca aggagaagga ctggtgcgtg atcaagaacg atgactgggg tgagatcatg
     6721 gagaaggtcc tcgactgcga ggtcctcgtc atcgggtcgc ccacctatta ctttgacgtg
     6781 tgcggccacc tgaagaactt catcgaccgc agctactcgc tctaccatcg aaggaaactt
     6841 gccgggagga aagcggtcgc agttgcagtc tgcgcaaaca agggtggtgc gaggaccatc
     6901 gagacccttg aagggttcct caactcccat gagttctcct accttggatg ggtacgtgga
     6961 aagggctacc tggaaggaga gataaccaaa gaccagaaag gcctgaagcg ggcccaggag
     7021 atcggcgata agatcgtgca actcctgaag cctcacgatt aatccttttt ttatcggcgc
     7081 aaggagaggt gcgggcccag cccgaagagc aaggcgggat ttggttgcat atcctgcccc
     7141 atgcagggat gaggaaaccg cgagagccgc cagggatggc ggcgctctgc gcataggatg
     7201 gtaaaaatcc tggatttcag gtgtgcccgg ggtcggtgta gagggttgtc ctctgcttga
     7261 gcgtccggcc gatgtccccg gccatgcggc gcatctcttc cgggtccagg tagtcggtgt
     7321 ccacggcccc ggcgcccttg gagatactct cctcgaacat cgtcccccca aggtcgtctc
     7381 cccctgcgag gagacagatc tgggccatct tgagaccgag tttgacccac gagacctgta
     7441 tatggggaat attgtcaagg aagagacgcg agacggcagt catcagcagg tcttcccttc
     7501 ccgtcgcacc cgcacgggcc agccccttcc ggaaaagcgg ggtctcccgg tgaatgaaac
     7561 tgagcgggac gaactccgtg aaccctcccg tctcgtcctg gatatccctg atgagcgcaa
     7621 ggtgcctggc ctggtcctcc actgtctcgc agtgcccgta catgatggtc gcagtcgaac
     7681 ggataccgag tgaatgggcc tccctgatga tccgcaccca gtctttggtt gacactttcc
     7741 ccgggcagat tacctccctg accctgtcaa ccaggatctc cgccgcagtt ccgcacagtg
     7801 agccgagccc cgcctccttc atcagtccaa gcatttcgat cgtcggaatg ccactctttc
     7861 ccgctccata tgcgacttcc atagggttgc ttgcatggat atggactcca ggcgcgccct
     7921 cacgtatgca ggagatgatc tccaggtacg atcccgcatc gaagtcaggg tgaagacctg
     7981 acaccgtgca gatctctgtc accccccgcg atcgggcgag cgccgccttc tccctcacca
     8041 cctctgccgg gaaatgatac gcatccttgt ctttgggttt tctggagaac ccgcagaatc
     8101 cgcaggagtt gatacagata ttggtgacgt ttatgttctg gttgcggacg taggtgactg
     8161 catcccccac cacgcgttcc ctctgttcat ccgcggcatt cgcaacccgc caga
//
LOCUS       DAOQYR010000093         8067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_9412_length_8067_cov_8.586745, whole genome shotgun sequence.
ACCESSION   DAOQYR010000093 DAOQYR010000000
VERSION     DAOQYR010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9412_length_8067_cov_8.586745"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..1418
                     /locus_tag="PKM87_09730"
     CDS             276..1418
                     /locus_tag="PKM87_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ30386.1"
                     /translation="MKLIHIADTHLGLSQFNRLDPESGINVREKQIYDNFLSSVDRII
                     SAKPDVLVHAGDLFDTVKPKTRAYTTVLEALERLHAAGIPLVVIAGNHSMVKTRYTTS
                     PFEVLVYHPGDIFAAYNFRYEHVEIGDTMFHLIPNMLRPGDYRDAYDEVEISMNHHNV
                     LVTHGLATQVRDKRLATVAEHELDSTILSDSFDYIALGHYHCQSQITGNAWYSGSIEF
                     LTYGEISDSKGGLFIDLDPLSVCPFPLPMTPMIDLGTIQCDGVRPGEISGEIISRITE
                     RHILPFSMVQVTLDGLSREHGKGPDMRDLVNVRERLLDLKIRARSPEENRVVPLQQDV
                     KAINYLEEFATFLDIQQIGARQKEYVARCGHEVLTTVMERHREDAE"
     gene            1483..3921
                     /locus_tag="PKM87_09735"
     CDS             1483..3921
                     /locus_tag="PKM87_09735"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HNQ30387.1"
                     /translation="MIIDRIVMKNFKRFRQQEIRFRDGITGILGNNGTGKSSIVEAVF
                     FSLYGVQATGLPSDYIVSSFAGQKEKCEVRLDFRVGGDEYTVLRSFKKGKSVQHDASF
                     YRAGKLRATGVSQVEAEVKRTLGMGPVDFRNTVYAAQKDLLTLLEHTSGKRKEWFLRA
                     LGIDYLKTESDSLLKERVDAKERELRLREGELKGLTDRHDPGARPQMENAMKDSKARL
                     QDLSRQAEDHAAGRRQVARLLQEFSEKKTEHTRLVERHLALITEVSGLSRQRDQISTR
                     IAAIAGQEQEYHMLERDLEHLDPKKERLTGLRAQKSEVDRLISEIKFIEQEQSAIEGR
                     IAKERKKIDSLERDSARLSALQGSIRELFKIGPEIPDSALEHAIASREADLQHSIGTV
                     SARLDHIVSERKKLVTDWNTIENAGPDGICPLCRQTLGAHFGRIGDEFSHRLEVIEEE
                     GLSLYGEQERLAEEKGLITQQRPALLEIRTITERLKGREAVEADLCELLSQAKAKGSA
                     LGSLKTRIRETGFNESVYMAAEKEVFELERLQARFIDVGKEIARARALKEQVKDLDIL
                     ITKKRGDIAALKASIDKSGFNPDTGTHLERDLAGIDSAIRAADAEIARVTERLRNIEE
                     NIERNRKEESAISGLKGKISALSEEIALLRLTRSLIGEYVLYLMQVVRIRIEGEVSRV
                     LAEITGGRYEQVLLDEDFNLLVRDIDDDYPLERFSGGEQDDIAVALRIALSRYLAELH
                     QVHESNLLILDEIFGSQDEERRNNLLSALRTQESRFPQIILISHIPDIQGEFANTLMV
                     EMGNDLSSRVQEVT"
     gene            3918..4949
                     /locus_tag="PKM87_09740"
     CDS             3918..4949
                     /locus_tag="PKM87_09740"
                     /inference="COORDINATES: protein motif:HMM:NF020933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HNQ30388.1"
                     /translation="MTANDRDFRDAADTTAKRIRSLVPGDLAEMFRQETKITPGEFHP
                     CPFVPPGEVCAVDGSNGLLLESGSMAIALFRAAQSTYHNRERHRRSLSPLRFGVIGPS
                     QENEDFYELYEECFGELPGTPLGNEDRSRVAGVLRDTLEYWITEQMAHSLSPGALLLR
                     DGPLRVSHASHDPVLSRIEDTCNARGISLVGVSKRTSATWGGGYPLVPTLAGLAEAFG
                     ISPPWWIRIDPNILDHAQYPQWQHGQMYVTCLHPHSKSPLKLELTAGIPEQRVREIMG
                     RLGACSMDGRIPGYPYPLLDAHRTVVLSEEIMEQARSDLIRRIVAAGMKLRTYEILFG
                     DYHDEFARY"
     gene            4927..6549
                     /locus_tag="PKM87_09745"
     CDS             4927..6549
                     /locus_tag="PKM87_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ30389.1"
                     /translation="MMNLRDIDRNDMSKYRLIGRSVLSYRFIAPYDTALYIGEIKKIS
                     DTTKDVTFFAKITDLSHDSNFADARWDTRVYAEEFYGLGEDVFLSVDAVPLGFVDNAT
                     GAFHKPRTIPSKFSTIGDPDAEDFRFLTNLMGEIEVGLMRSGQDVIRDVAVRIPSRIL
                     PQHMGVFATTGMGKSNFMRVFCASCMKERKFGLLIVDPHGEYVSGGRSSIGEPTSGLV
                     HYTEGKDGLSIFTIRHETDRKKYGLNRLSIEYDDFRIGDLAILYDLSAAQAEIVEAFA
                     GVSGSEVIGFFRAIDPEIFPIRDPVAAGVAEGSLAWKIRNSMGGPVRVIKSHIENIVN
                     GNRTFFKPKGSSLSEIISHLHENRVVLVDIPDMSERSELFVLSIITRTIMERHRSEAR
                     SFGIDERQESSHQVLITIEEAQRVLASGGASTQVFRECAMEGRKFGVGLCVITQQPKN
                     IDPKVLAQINTFVVMGLGDRGDRDIIMGSAKQDLSKMEIEIQTLDRGEAIISTIGIPF
                     PVSTRIHRFEDYIAALNRGKTFRLSDGLEEKF"
     gene            6755..6940
                     /locus_tag="PKM87_09750"
     CDS             6755..6940
                     /locus_tag="PKM87_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30390.1"
                     /translation="MVSRPAIDAVFEAMFILTDIRVMLRETAPGHVFSPAQREEASAL
                     LNGLENKVEILRRELLQ"
     gene            6937..>8067
                     /gene="pscS"
                     /locus_tag="PKM87_09755"
     CDS             6937..>8067
                     /gene="pscS"
                     /locus_tag="PKM87_09755"
                     /EC_number="2.5.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-phospho-L-seryl-tRNA:Cys-tRNA synthase"
                     /protein_id="HNQ30391.1"
                     /translation="MKCAGPIEVRDLDESSINIDPIQVGGRLTPEAMKAVIAYGDGYS
                     ICDHCRKPFRLDFIKTPPVDVFFRELAQFLNMDVARVVPGARRGFQAVADTYVQKEDP
                     VLITALSHYTEVLSVEARGGLVREIPKTEGNLINPDATAEKIEAVMRETGKTPPLLFI
                     DQVDYQYGNLHPVKEITRVAHQYDIPVIVNGAYTVGIMEVDGKEIGADFVVGSGHKSM
                     ASPAPSGVLAATEERAAEVFRTTTVKTDVTSRTFGIKEVEMMGCTLMGVTLVGMMASF
                     PRVRDRVKRWDQELSNGNLVVEALRRITGTEIRSEYPRNHTLTRVDTTRSFDEVAKTH
                     KKRGYFLSSALKARGIAGTIPGATKVWKFNTYGMTQAQAEYVA"
BASE COUNT         1910 a         2232 c         2320 g         1605 t
ORIGIN      
        1 ctcaaacggc ggggggtctg gtagcggtag ctatccaaga ttttagagaa aacacgtgca
       61 attcaaacga tttgatgaaa tcgagaataa cattcttggt ttccggagga taatccgggg
      121 cattttataa tgggccttgt gtcatcccct gtttttcgat attgtcacaa gggatttatg
      181 caggtttact gctgatgacc cccaatagca gcattacttt cacccccctc cccctcccac
      241 ttataaccat cacagtccaa tgagaaacat gagaaatgaa gctgatccat atcgccgaca
      301 cccacctcgg cctctcgcag ttcaaccggc tcgacccgga atccgggata aatgtgcggg
      361 agaagcagat atacgacaat tttttatcgt cggttgacag gattatctct gcgaagcccg
      421 acgtgcttgt acatgccggc gaccttttcg acacagtgaa gccgaagacg cgggcttaca
      481 cgactgtcct tgaagcactc gaacgactcc atgccgcagg gatccccctc gtcgtcattg
      541 caggaaacca cagcatggtc aagacccggt acacgacttc cccgttcgaa gtgctggtct
      601 accacccggg cgatatcttt gcggcgtata acttccgata cgagcacgtg gagatcgggg
      661 atacgatgtt ccacctgatc ccgaacatgc ttcgacccgg tgactaccgg gatgcatacg
      721 acgaggtgga gatctctatg aaccatcaca atgtgttggt gacccatggg ctcgcaacgc
      781 aggtcaggga taaacgactt gctaccgtag cggagcacga acttgacagt accatcctct
      841 ccgactcgtt cgactatatc gcgctcggtc actaccactg ccagtcgcag atcacgggca
      901 atgcctggta ctcgggatca atagaattcc tcacctacgg agagatatcg gattcgaaag
      961 gaggcctttt catcgacctt gacccgctct ctgtctgccc ttttcccctt cccatgaccc
     1021 cgatgatcga ccttgggacg atccagtgcg atggagtcag accaggcgag atctcgggcg
     1081 agataatctc gcggattacg gaaaggcaca tcctaccctt ctcgatggtt caggtcacgc
     1141 tcgacggcct ctcccgcgaa catggaaagg gtcccgatat gagggacctt gtcaatgtcc
     1201 gcgaaaggct gctcgatctc aagatcaggg cccggtctcc agaagagaac cgggtggtgc
     1261 ccctccagca ggatgtaaag gctatcaact accttgagga gttcgccacg tttcttgata
     1321 ttcagcagat cggcgcaagg cagaaagagt atgtcgcaag gtgcggccat gaggtgctga
     1381 ccacagtgat ggaacgccac cgggaggatg cagagtgacc ccccaaagag acgctttccc
     1441 tggagaccgt ttccaccacg attacccgaa cggaggcatg ggatgatcat cgaccggatc
     1501 gtcatgaaga acttcaagcg gttcaggcag caggagatcc ggttcaggga cggaattacc
     1561 ggcatcctcg gaaacaatgg gaccgggaag agcagtatcg tggaagcggt cttcttttcc
     1621 ctctatggcg tacaggcaac gggcctcccg tccgactaca tcgtctcatc gtttgccggt
     1681 caaaaggaga agtgcgaggt gcggctcgac ttccgcgtcg gtggtgacga gtatacggtc
     1741 ctccgctcgt tcaaaaaagg aaaaagcgtc cagcacgacg catcgtttta ccgggctgga
     1801 aaacttcgtg caaccggggt gagccaggta gaggccgaag tgaagaggac gcttgggatg
     1861 gggcccgtcg attttcgaaa cacggtttac gcagcccaga aagacctcct tacgctcctt
     1921 gagcatacat cggggaagag gaaagaatgg ttcctgcgtg cactgggcat cgattacctg
     1981 aagaccgaga gcgacagtct cttgaaagag cgggttgatg cgaaagagcg ggagcttcgg
     2041 ctgcgagagg gggaactcaa ggggctcacc gaccggcatg acccgggcgc ccggccacag
     2101 atggagaacg caatgaaaga ctcgaaagcc aggctgcagg atctgtcacg acaggccgag
     2161 gatcacgcgg caggaaggcg gcaggttgcc cgtctgctcc aggagttctc cgaaaagaag
     2221 acggagcata cccgcctcgt tgagcggcac cttgcactta tcacagaggt gtcaggactc
     2281 tctcgtcaga gggatcaaat atccacccgg attgccgcca tcgcgggtca ggaacaggag
     2341 taccatatgc tcgagcgcga cctggaacac cttgacccga agaaggaacg gcttacagga
     2401 ctgagggcgc aaaaatctga ggttgaccgg ctcatatccg agataaagtt tattgagcag
     2461 gaacaaagcg caattgaggg gagaattgca aaagagagga aaaagatcga ttccctggag
     2521 cgagattcgg cccgattgtc tgccctgcag gggagtatcc gcgaactctt taaaattggg
     2581 ccggagatcc cagattcggc actcgaacat gctatcgcat ccagggaggc ggatcttcag
     2641 cactccatcg ggactgtatc ggcaaggctc gaccatattg tcagcgagag aaagaagctt
     2701 gtaaccgact ggaatacgat cgagaatgcc ggccccgatg ggatctgccc gctctgccgc
     2761 cagacgcttg gggcacattt cggcaggata ggggatgagt tctctcacag gctcgaagtg
     2821 atcgaggagg aagggctctc gttatacggc gagcaggaga gactggccga agagaagggt
     2881 ctgattaccc aacagagacc ggcccttctt gagatccgga ccatcaccga gagactgaaa
     2941 ggcagggaag cagtcgaggc tgatctttgt gaacttctct cccaggcgaa agcaaagggg
     3001 tcagcccttg gatctctcaa aacccggata cgggagactg gcttcaacga atctgtatac
     3061 atggctgcgg aaaaggaggt ttttgaactg gaacgtctcc aggcacgatt catcgacgtc
     3121 gggaaggaga ttgcccgggc ccgggctctg aaggaacagg taaaagacct cgacatcctc
     3181 attacaaaga agagaggaga tatcgctgca ctcaaggcct cgatcgacaa gtcagggttc
     3241 aatcccgata cagggactca tctcgagcgg gaccttgccg gtatagactc ggcgatacgt
     3301 gcagctgatg cagagattgc acgggtaacc gagcgtctcc ggaacatcga ggagaacatc
     3361 gagaggaacc ggaaagaaga gtccgcgatt tccggcctga aagggaagat ttctgcactc
     3421 tctgaagaga ttgccctcct ccggcttacc cgctccctga ttggggagta tgtgctctac
     3481 ctgatgcagg tggtccgaat caggatcgag ggcgaagtca gccgtgtcct cgccgagatc
     3541 acgggcggaa ggtacgagca ggttctcctc gatgaggact tcaacctcct cgtcagggat
     3601 atcgacgatg actaccctct cgaacgattc agcggcgggg agcaggacga catcgcggtt
     3661 gcgctcagga ttgcactctc gcggtatctc gcggagcttc accaggtgca cgagagtaat
     3721 ctcctgatct tagacgagat cttcgggagc caggacgagg aacggcggaa caatctcctc
     3781 tccgcgctca ggacacagga gtcccgtttt cctcagatca tcctgatatc ccatataccc
     3841 gacatccagg gagagtttgc gaacacgctg atggtggaga tgggaaacga cctttcgagc
     3901 cgggtacagg aagtgacatg acggcgaatg accgtgactt ccgggatgcg gcagatacca
     3961 ctgcgaagcg catcaggagc ctcgtgcccg gagatcttgc cgaaatgttc aggcaggaga
     4021 caaaaatcac gccgggcgag ttccacccat gcccctttgt tccaccaggg gaggtctgcg
     4081 cggtggatgg gagcaatggg ctcctgctcg aatcgggaag tatggctatc gcgctgttcc
     4141 gtgccgccca gagcacttac cacaaccgcg agaggcaccg gagatctctc tctcccctgc
     4201 ggttcggagt cattggtccc tctcaggaga acgaggactt ttacgaactc tacgaggaat
     4261 gcttcggcga attgccgggg actcccctcg gaaacgagga tcggagccgg gttgcagggg
     4321 tccttcggga caccctcgaa tactggatca cggaacagat ggcgcattcc ctctcgcccg
     4381 gtgcccttct cctccgcgac gggccccttc gtgtctcaca tgcgagccac gaccctgtcc
     4441 tctccaggat cgaggatacg tgcaacgcga gaggtatcag cctggtcggg gtgagcaagc
     4501 ggacatcagc cacctgggga ggcggctacc cgcttgtccc aacccttgcc ggactcgcag
     4561 aggcgtttgg gatatccccc ccctggtgga tccgtatcga ccctaatatc cttgaccacg
     4621 cccagtatcc ccagtggcag catgggcaga tgtatgtcac ctgtctgcat ccccactcaa
     4681 agtcaccatt gaaactggag ctgacagccg gcatccccga acagcgggtc cgggagatca
     4741 tggggcgcct tggggcctgt tccatggatg gccgtatccc tggatacccc tatcccctcc
     4801 ttgatgccca tcggacagtg gtgctcagcg aggagatcat ggagcaggca agatccgacc
     4861 tgattcgacg tatcgtcgca gcagggatga aactgcggac atacgagatc ctctttggag
     4921 attaccatga tgaatttgcg cgatattgac aggaatgaca tgtccaagta ccggttgatt
     4981 gggcgatcgg tcctctccta ccggtttatt gcaccctacg acacggcgct ttacatcgga
     5041 gagatcaaga agatctccga taccacgaag gatgtgacct tctttgcaaa gatcaccgac
     5101 ctctcccacg acagcaactt cgctgatgcc cgctgggaca cccgcgtata tgccgaggag
     5161 ttctacggtc tcggagaaga cgtctttctc tctgtcgatg ctgtccctct cgggttcgtg
     5221 gataacgcga caggggcatt ccacaagccg cggacgattc cctcgaaatt ctccacgata
     5281 ggggatcctg atgcggagga cttccgtttc ctcaccaacc tgatgggaga gatcgaggtc
     5341 ggcctgatga ggagcggaca ggacgtaatc cgcgacgtcg ccgtgcgtat cccaagccgc
     5401 atcctccccc agcacatggg tgtatttgcg acgaccggga tgggcaagag caacttcatg
     5461 cgggtctttt gcgcctcctg catgaaagag agaaaattcg gcctcctcat tgttgatccg
     5521 cacggtgaat acgtcagcgg cgggaggtca tcgattggcg agccgacctc cggccttgtc
     5581 cactacacgg aaggaaaaga cgggctctcc atcttcacca tccgtcacga gaccgacagg
     5641 aaaaagtacg ggctgaaccg gctctcgatc gaatacgacg acttccggat cggcgatctt
     5701 gctatccttt acgacctctc tgcagctcaa gccgagatcg tcgaggcgtt cgcgggtgtc
     5761 tccgggagcg aggtcatcgg ttttttcagg gcaatcgacc ccgagatctt tcccataagg
     5821 gacccggttg cggccggagt tgcggagggg agccttgcat ggaagatccg gaattctatg
     5881 ggtgggcctg tccgggtgat caagagccat atcgagaaca tcgtgaacgg taacaggact
     5941 ttcttcaagc cgaagggatc gtccctttcc gagatcatct cccacctcca tgagaaccgc
     6001 gtggtgctgg tagacatccc cgacatgagc gagaggagcg agctcttcgt gctctcgatc
     6061 atcacccgca cgatcatgga gaggcaccga tccgaggcac gttcattcgg gatcgacgag
     6121 cgccaggagt cctctcacca ggtccttatc acaatcgagg aggcgcagcg tgtgcttgca
     6181 agcggcgggg catccacgca ggtattcagg gagtgtgcca tggaagggag gaaattcggg
     6241 gtcgggctct gcgtgatcac ccagcagcca aagaacatcg atcccaaggt gcttgcccaa
     6301 atcaacactt tcgtggtcat gggacttggg gacaggggtg accgcgacat catcatgggc
     6361 agtgcaaaac aggacctctc gaagatggag atcgagatcc agaccctcga ccggggtgaa
     6421 gcgatcatca gcaccatcgg catcccgttc ccggtcagca cccggattca caggttcgag
     6481 gactacatcg cggcattgaa ccggggaaag accttccggc tctcggacgg acttgaggag
     6541 aagttttgat caagaacctg gaacctctcc ccacaacctg tgtcaagggg ttgaaaatta
     6601 tctctgtcga tggaaaagtt accgctcaca tcgcatccat accgatcccc gcgataaaag
     6661 accaatctga actatagtgt cttcgcaagt cagcccaggt tacaccggtt taccgggtat
     6721 cttaatgccg aggtattcat attgatatgg tgatatggtg tccagaccgg caatcgatgc
     6781 agtattcgag gcaatgttca tcctgaccga tatccgggtg atgcttcgcg agaccgcacc
     6841 cggccatgtc ttttcccctg cccagcgcga ggaggcatcc gcactgctga atggccttga
     6901 aaacaaggtc gagatcctgc gccgggagct cctgcaatga aatgcgcggg cccaatcgag
     6961 gtacgggacc ttgacgagtc atccatcaat atcgacccta tacaggtggg ggggcgactg
     7021 accccagagg ctatgaaggc cgtcatcgca tacggtgacg ggtattcgat ctgcgaccat
     7081 tgccggaagc ccttccgcct cgatttcatc aagacccccc cggtggatgt tttcttccgg
     7141 gagcttgcgc aattcctgaa catggatgtg gcacgagttg tccccggcgc ccggcgcggg
     7201 ttccaggcag tagctgacac gtatgtccag aaggaggacc ccgtgctcat cactgcactc
     7261 tcgcactaca ccgaggtact ctcggtcgag gcccggggag gacttgtccg cgagatccca
     7321 aagaccgaag ggaacctgat caaccccgat gccactgccg aaaagattga ggcagtcatg
     7381 cgcgagacag gaaagacccc gcctcttctc ttcattgacc aggttgacta ccagtacggg
     7441 aatctccacc cggtaaagga gatcacaaga gtcgcacacc agtatgacat tcctgtcatc
     7501 gtaaacggcg catacacggt cgggatcatg gaagtggacg gaaaagagat tggcgccgat
     7561 ttcgtggtgg gttcgggtca caagagcatg gcatccccgg caccttcggg tgtcctggcg
     7621 gcaacggaag agcgggcggc agaggtattc cgcactacca cggtaaaaac cgacgtgaca
     7681 tcccggacgt ttggaataaa ggaggtagaa atgatgggat gcactctcat gggagtcact
     7741 cttgtcggga tgatggcctc ttttcccagg gtccgtgacc gggtgaagcg ctgggaccag
     7801 gaactttcga atgggaacct ggtggtggag gcattgaggc gtatcaccgg caccgagatc
     7861 cggagtgagt atcctagaaa tcataccctt acccgtgtgg acaccacgag atccttcgac
     7921 gaggtggcaa aaacccataa aaaacgcggg tacttcctct caagcgccct gaaagcaagg
     7981 ggcatcgccg gcacgattcc tggtgcaaca aaagtctgga agttcaacac ctacgggatg
     8041 acgcaggcac aggcagaata tgttgcc
//
LOCUS       DAOQYR010000094         8055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_9430_length_8055_cov_8.728875, whole genome shotgun sequence.
ACCESSION   DAOQYR010000094 DAOQYR010000000
VERSION     DAOQYR010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 8055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8055
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9430_length_8055_cov_8.728875"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /gene="mch"
                     /locus_tag="PKM87_09760"
     CDS             complement(<1..803)
                     /gene="mch"
                     /locus_tag="PKM87_09760"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HNQ30392.1"
                     /translation="MLSVNELALEIFENLAEYAEEFNAAYHELDNGARIIDCGVSTRG
                     GYAAGRAFTEICMGGLGEVNFRMANIKDFPLPFIDVFTDFPSISCLGAQKAGWTIKHN
                     NYFAMGSGPARALSLKPKHTYEVIEYEDDFDSAVIALESDHLPNGEVMEKIAEDSHVD
                     VANVCAVVAPTASIVGSIQVAGRCVETAIYKLNELHFDTRKIVAAFGCAPLPPVRGPK
                     MAMGVTNDATIYHGRIMLTMKAPEIKDYLEKIPSNKSKGYGKPFNEIFK"
     gene            complement(879..2003)
                     /locus_tag="PKM87_09765"
     CDS             complement(879..2003)
                     /locus_tag="PKM87_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORC1-type DNA replication protein"
                     /protein_id="HNQ30393.1"
                     /translation="MKKNLLMWDETLFRDPEVFEIDYVPEQFNFRETQMRELAFQIRP
                     GLRGGRPLNTICRGLPGTGKTTSVKKLFAEIEETTKKLVPIHVNCQIDNTKFAIFSQI
                     YRKLSGHLPPSSGTSFKQVFDAIARILAKEEQVLLVALDDANYLLYENEINKVLYTLL
                     RSHESYPGTRVGVIMIVSDMAVDIASEVDARVSSVFRPTEIYFPPYTMEEVKSIISER
                     VMQGLYPGCLPPGMFDLVVEQTQKSGDLRVGVDLLKRAALNAEKRASRVIERDDICGA
                     YQVSRYLHLAYSVKSLKEEEKALLRTLAIHAGQGGEMNAGEVYKTVKEEAGIGYTRFY
                     EIVKKFDAMRLVNLEYRDGKGRTRVISLRYEPVKILEYLS"
     gene            complement(2056..2553)
                     /locus_tag="PKM87_09770"
     CDS             complement(2056..2553)
                     /locus_tag="PKM87_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30394.1"
                     /translation="MSGPKSLTIGITVNLEHYENLRLEVNGEVKDQDDARDLARFLSD
                     ILVTYGRGDPATAERIENFKKRVLPVIDERSEAQLTLPQEGARPSGASVTSAETPSPV
                     SPEIAATPPPPHPAPPAAPAQESGAAAGGATCEECGVPVTQAEQKMSMLFASRVLCRK
                     CLKKV"
     gene            complement(2614..4146)
                     /locus_tag="PKM87_09775"
     CDS             complement(2614..4146)
                     /locus_tag="PKM87_09775"
                     /EC_number="2.4.2.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ribofuranosylaminobenzene 5'-phosphate
                     synthase"
                     /protein_id="HNQ30395.1"
                     /translation="MNEAWLFDELERVAGPLTPLQRILLGTDGSVTRILELATGAPVA
                     ITTLQQTVEAADLLVAQRLDVPPGQKVNHRIVELKNTRTHETLIYAESFTPLSRLSPS
                     FRDDLMRADIPIGKILEQHQLETRREIVKMSAGPRPAPVAESFGLSGNPRFLSRQYRI
                     IHHEHPLIHIEEVFPAGLFSGEIRVVIDAPSRLHLGLLDMNGSLGRIDGGIGLALDEP
                     RLVLSARKSEAFAVEGGDADARERVLAAAAAVSKSLNLPGAAEFVVQEDFPGHVGLGR
                     GTQLALSTACALCRLYGQEWNTRDLARMTGRGGTSGIGTASFAAGGFLLDGGHSFGVV
                     MEKAAFLPSSASRGVRPPEVLLRREFPEAWKVLLVIPDVPPGASGGTEKDLFSRYCPV
                     PLEEVRELCHLVVASLLPGLAEEDLDLFGSAINRMQELGFKKVENTLQAPGVGDLMRA
                     MREAGAAGAGLSSFGPAVYAFGEGRMHDVESAALEILPSLGGGRVLLTRARNSGAVVS
                     MA"
     gene            complement(4221..5384)
                     /locus_tag="PKM87_09780"
     CDS             complement(4221..5384)
                     /locus_tag="PKM87_09780"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HNQ30396.1"
                     /translation="MQKKYIVILGDGMADEPIPEIGDRTPLEAARTPHMDRIARDGCC
                     GLLRTVPNGCEAGSDIANLSILGYDPRRYYTGRGPLEAASMGIPLGPSDMAYRCNLAT
                     VKDGVMADFSAGHITSAEGASLLASLDREISPARVYPGVSYRNLLVVPDAGGAKTVPP
                     HDIVGQEVAPHLPSGRDAPLLLECMEASQRIFAGHPVNRDRVSRGLLPATQIWPWSGG
                     RVPLLTPFFEKYGKRGGIISAVDLLKGIGRCAGMEIIEVPGATGYLDTDYQAKARYAL
                     FALEHLDFVYIHVEAPDEAGHLGSIKEKVTAIERLDEMIGTIMKEFHGVVAVLPDHPT
                     PIRIRTHSGDPVPFAIFGKGRDGCTRFSEKAAAGGVCGLMEGPDFLSFLFCED"
     gene            complement(5410..6357)
                     /locus_tag="PKM87_09785"
     CDS             complement(5410..6357)
                     /locus_tag="PKM87_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNQ30397.1"
                     /translation="MKAVVTGGAGFIGSHLVDALVTRGDEVCVIDNLSTGDLRHIAGH
                     IDSGAVRFVRGDLLEEGWQSSIRDANRVYHIAADPDVRASAGNPAGVYARNVTTTARV
                     LEAMREAGCREIAFTSTSTVYGEADIIPTPEDYPGMRPISIYGATKLACEAMISGYAY
                     TYGWNSWVFRFANIIGERSNHGVIWDFAHKLRKNPRELEILGDGRQAKSYCSVSACVD
                     AVLFAVTHAHEPFACFNIGTEDWIDVTSIASIVAAEMGLERVSFRYTGGDRGWVGDVP
                     RMLLSVERLKSLGWRPLVTSETSVREAARALIRESGHFP"
     gene            6414..7298
                     /locus_tag="PKM87_09790"
     CDS             6414..7298
                     /locus_tag="PKM87_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HNQ30398.1"
                     /translation="MVSRIDAPQGGLVFFKGVLMGMCDIIPGVSGGTIALITGIYERL
                     ISAIGSIGISPLRALIRGRREEAVDAIHRMDPVFLVVLMAGIGAGLLAMSRIVLSVLQ
                     AFPAETYAFFFGLIVASSVLIAYRIERWDTVIATVWVGLGLAAGYLIGGLGQSGLGHS
                     LPMLFVTGFVALCAMILPGISGAYLTLVMNQYEYLLSAIATLDIVPLLAYIAGGAAGI
                     ISMSRILKIILRTYHTQSLAFLTGLMLGSTRMMYDHIVAAGGTIASVLVAGVAGVLLI
                     ALIELALRLFSKRSPDPE"
     gene            7373..>8055
                     /locus_tag="PKM87_09795"
     CDS             7373..>8055
                     /locus_tag="PKM87_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 12 protein"
                     /protein_id="HNQ30399.1"
                     /translation="MYVGIDHGTSAIRFSAGDGRRFKVRREDAVSFRIQDLSRLAPLD
                     EIDGIALTYSMGDNFSAITPIRDLENRGLVSREGAGKHTGGGTRVFDQVAGSSIPAVA
                     IPGLHRSSPTDPRFSVYSHQASPEKIGIAFRVVQDLGGDVVISDVSSNTVSILVRDRR
                     LVGALDACIFAPGTEHGALDVDAIRRVDRREMSANEAFLHAGVNHTLSPERRIPALAL
                     FSAMECASLL"
BASE COUNT         1491 a         2452 c         2433 g         1679 t
ORIGIN      
        1 tccttgaaga tctcgttgaa gggttttccg tatcccttgg acttgttgct tgggatcttc
       61 tccaggtaat ccttgatctc gggggctttc atggtcagca tgatcctgcc atggtagatg
      121 gtggcgtcgt tggtcacacc catggccatc ttcggtcccc tcacgggggg aaggggagcg
      181 cagccgaacg cggcgacgat tttcctcgta tcgaagtgga gttcgttcag cttgtagatc
      241 gcagtctcca cgcaccgccc ggcgacctgg atggaaccaa cgatggatgc cgtaggggca
      301 acgacagcgc agacattggc gacatccacg tgggagtctt cggcgatctt ctccatcacc
      361 tcgccgttcg ggaggtggtc gctttcgagg gcgatgaccg ccgaatcgaa gtcgtcctcg
      421 tactcgatca cctcgtaagt gtgcttgggc ttgagcgaga gggctctcgc cggcccgctg
      481 cccatggcga agtagttgtt gtgcttgatc gtccatcccg ctttctgggc gccgaggcag
      541 gagatggagg ggaagtccgt gaacacgtcg atgaaaggaa gggggaaatc cttgatgttg
      601 gccatcctga aattgacttc accgagacca cccatgcaga tctcggtaaa ggcgcggccg
      661 gccgcgtacc ccccacgggt gcttaccccg caatcgatga tacgggcccc gttatcgagt
      721 tcatggtatg ctgcgttgaa ttcttcggcg tattccgcca ggttttcaaa aatctctagg
      781 gcaagttcgt taacactgag cacgtggagt acccccttta ccatagaaca tagcaggaga
      841 tgagataaga gttatcaaat gatcgtttta taaaaagatc aggagaggta ttcaagtatt
      901 tttaccggtt cgtacctgag gctgatgacc cgggtgcgtc cttttccgtc ccggtattcc
      961 aggttcacga gccgcatggc atcgaacttc ttcactatct cgtagaacct ggtatatcca
     1021 atacctgcct cttctttcac cgttttatat acctcgcctg cgttcatctc ccctccctgg
     1081 ccggcatgga tggcaagggt ccggagcagg gctttctcct cttccttgag gcttttcacc
     1141 gagtatgcca ggtgaaggta gcgcgatacc tggtaggcgc cgcagatgtc gtccctctct
     1201 atcacgcgac tggcccgttt ctccgcgttc agggcggccc gtttcagcag gtcgacccct
     1261 accctgagat ccccgctctt ctgggtctgc tccactacca ggtcgaacat cccgggaggg
     1321 agacatccgg gatagagccc ctgcatcacg cgctcgctga tgatgctttt cacctcctcc
     1381 atcgtgtaag gagggaagta gatctctgtc gggcggaaga ccgaggagac ccgtgcatcc
     1441 acttcgcttg caatatccac ggccatgtcg ctgacgatca tgatcacccc caccctcgtt
     1501 cccggatacg actcgtgcga gcggaggagc gtgtaaagca ccttgtttat ctcgttctcg
     1561 tagaggaggt agttcgcgtc gtcgagcgcc accaggagca cctgctcctc ctttgcaagg
     1621 atcctggcga tggcatcaaa gacctgcttg aaggaggtgc ccgaggaagg tggcaggtgg
     1681 cccgagagct tccggtaaat ctgggagaat atggcgaact tggtgttgtc tatctggcag
     1741 ttgacgtgga tcgggacgag cttctttgtg gtctcctcaa tctcggcgaa gagtttcttc
     1801 acgctggtgg tcttcccggt ccccggaagg ccgcggcaga tggtgttgag ggggcgcccc
     1861 ccccgcaggc ccggccggat ctggaaggcc agctcccgca tctgtgtctc ccggaagttg
     1921 aactgttccg ggacatagtc gatctcaaag acctcggggt cccgaaagag ggtctcgtcc
     1981 cacatcagga ggttcttctt caccggggca ccacctttct tttccggctc attgcgacct
     2041 gtaccgactc gcgcgtcaga ccttcttgag gcatttcctg cataagaccc tgcttgcaaa
     2101 gagcatgctc atcttctgtt ctgcctgggt caccggcacg ccacattcct cgcaggtcgc
     2161 acctcctgcc gccgcgccgc tctcctgcgc gggcgcggca ggcggagcag gatggggagg
     2221 gggcggagtc gcggcgatct caggagatac cggggagggt gtttccgcag atgtaacact
     2281 ggctccggaa ggacgcgctc cttcctgggg aagtgtgagt tgtgcttcgc tgcgctcgtc
     2341 aatgacgggg aggacgcgct ttttgaagtt ctcgatccgt tcggcggtcg cggggtcgcc
     2401 gcgcccgtag gttacaagaa tgtctgagag gaaacgggcc aggtcgcggg cgtcatcctg
     2461 gtccttcacc tcgccgttca cttcgaggcg gaggttctcg tagtgctcca ggttcactgt
     2521 gatcccgatg gtgagtgact tcggcccgct catagtacca tactggagca gtatttctaa
     2581 tgaaagtgcc tccggcccgg ccatgaccgg gggtcaggcc atgctgacca ctgcgccgga
     2641 atttctcgcc cgcgtgagga gcacgcgacc tcccccgagt gaggggagga tctccaatgc
     2701 cgcactctcc acgtcatgca tgcgcccctc cccgaatgca tacaccgcag gcccgaacga
     2761 agagagcccc gcacccgcgg cacccgcctc ccgcatcgcc cgcatgaggt cgccgacccc
     2821 tggagcctgg agggtattct cgaccttctt gaacccgagt tcctgcatcc ggttgatggc
     2881 agaaccaaag aggtccaggt cctcctcggc aaggccgggg aggagcgagg ccaccacaag
     2941 gtggcagagt tcgcggacct cctcgagagg cacgggacaa taccgggaga agagatcctt
     3001 ctcggtccct ccgcttgccc cgggcgggac atccgggatg acgagcagga ctttccaggc
     3061 ctccggaaat tctcgccgca ggagcacctc tggcgggcgg accccccggg aggccgacga
     3121 cgggaggaat gcggccttct ccatgaccac gccaaacgaa tgccccccgt caaggagaaa
     3181 tcctcctgcc gcaaacgatg cagtcccaat ccctgaagtg cccccgcgac cggtcatgcg
     3241 cgcaaggtca cgggtattcc actcctggcc ataaaggcgg cagagagcgc atgcggtcga
     3301 gagggcaagc tgcgtcccgc ggccgagtcc cacgtggcca gggaagtcct cctgaacgac
     3361 gaactccgcg gcacctggaa gattcaggga ttttgagacg gctgccgccg cggcaagtac
     3421 cctctcccgg gcgtctgcat cgccaccttc gacggcgaac gcctcgctct tccgtgcaga
     3481 gaggacgagg cgtggctcgt cgagcgcaag cccgattccc ccgtcgatcc tcccgagcga
     3541 gccgttcatg tcaaggaggc ccaggtgcag cctcgagggt gcatcgatca ctacccgtat
     3601 ctctcccgag aagagcccgg cagggaagac ctcctcgatg tgaataaggg gatgctcatg
     3661 gtggatgatc cggtactgcc tggagaggaa ccgcgggttt cccgagaggc cgaaagactc
     3721 ggcaacgggt gcaggccggg gcccggcgga catcttcacg atctcccgcc tcgtctccag
     3781 ctgatgctgc tcgaggatct tcccgatcgg gatgtcggcg cgcatcaggt cgtcccggaa
     3841 agacggggag agcctggaga gcggggtaaa ggactccgcg tagatgagcg tctcatgtgt
     3901 ccgggtgttt ttcagctcca cgatacggtg gttgactttc tgccctggcg ggacatccag
     3961 tctctgcgca accagcaaat ccgctgcctc caccgtctgc tgcagggtgg tgatggcaac
     4021 cggtgccccg gttgccagtt ccaggatcct tgtcacagac ccgtcagtcc caagcagtat
     4081 cctctgcagc ggggtgagcg gtccggcaac ccgttcgagt tcgtcgaaca gccaagcctc
     4141 gttcatgcca caccagtgat ggtggtgggg agatacaagc tttccccctg gatcttcgac
     4201 cgcggaaggt tcgggaagcg tcagtcctcg cagaagagga aggagaggaa gtcaggaccc
     4261 tccatcagtc cgcacactcc tcccgcggcc gccttctctg agaaacgggt gcagccgtcc
     4321 ctgcccttcc caaagatcgc aaagggcacg gggtcgcctg aatgggtccg gatccgtatc
     4381 ggggtcgggt ggtcggggag cacggcgacg acaccatgga actccttcat gatggtgccg
     4441 atcatctcat ccagccgctc gatggcagtc accttctctt tgatgcttcc caggtgccct
     4501 gcctcgtcag gtgcctcgac gtggatatag acgaagtcca ggtgttccag cgcaaagagt
     4561 gcataccggg ccttggcctg gtagtcggtg tcaaggtacc cagtcgcgcc ggggacctcg
     4621 atgatctcca ttcccgcgca cctccctatc cccttgagga ggtcgactgc ggatatgata
     4681 cccccccgct tcccgtattt ctcaaaaaaa ggggtgagca ggggaaccct gcccccgctc
     4741 cagggccata tctgggtggc cgggaggagt ccgcgggaaa cgcggtccct gttcacaggg
     4801 tgtcccgcaa agatcctctg cgaggcctcc atgcactcca ggagcagggg agcatccctg
     4861 cccgaaggga ggtggggcgc gacttcctgg cccacgatgt cgtggggagg gacggtcttt
     4921 gctccccctg catccggcac gaccaggagg ttgcgatagc tcacgcccgg gtagacccgt
     4981 gcaggagata tctcgcggtc aagtgatgca agaagcgatg cgccctcggc gctcgtgatg
     5041 tgcccggcag agaagtctgc catgaccccg tccttgacag tggcgaggtt gcagcggtag
     5101 gccatatccg agggaccgag gggtatcccc atgcttgccg cctcgagcgg gcctctgccg
     5161 gtgtagtagc ggcggggatc atatccgagt atcgagaggt tggcaatgtc ggatcctgcc
     5221 tcgcacccgt tgggaacggt ccgcaggagg ccgcagcacc cgtcccttgc aatgcggtcc
     5281 atgtgcgggg tacgcgcagc ctcgagcggt gtccggtccc cgatctccgg gatcggctcg
     5341 tccgccatcc cgtctccgag aataacgata tacttcttct gcatgtctgt cctgcctgta
     5401 tcgcccggtt caggggaagt gacccgattc ccggatcagc gcccgggcag cctcccggac
     5461 agatgtctcc gaagtcacca ggggcctcca gccgagcgat ttgagccttt caaccgagag
     5521 gagcatacgg gggacgtccc ccacccaccc gcgatcgccg cctgtgtatc ggaaggacac
     5581 gcgctcaagc cccatctccg ctgccacaat ggacgcgatc gaggtcacgt cgatccagtc
     5641 ttccgtcccg atattgaagc aggcaaaggg ttcgtgtgca tgagtgacgg caaacaggac
     5701 cgcatccacg caggccgaca ccgagcaata ggacttcgcc tgccgaccat caccgaggat
     5761 ctccagttcc cgcggattct tgcggagctt gtgtgcgaaa tcccagatga cgccatggtt
     5821 gctcctctcg cctatgatgt ttgcgaacct gaacacccac gaattccagc cgtatgtata
     5881 ggcgtaaccc gagatcatcg cctcgcaggc gagttttgtc gcgccgtaga tggagatggg
     5941 tcgcataccc gggtaatcct cgggcgtagg gatgatgtcg gcttcgccat acactgttga
     6001 ggtagaggtg aatgcgatct ccctgcaccc tgcctcccgc atggcctcga gcaccctggc
     6061 cgttgtcgtc acgttccggg catacacccc tgccgggtta ccggcactgg ccctgacatc
     6121 cgggtctgca gcgatgtgat agacacgatt cgcatcccgg atagagctct gccagccctc
     6181 ttcgaggaga tctcccctga caaacctgac cgcgccggaa tcgatatgcc ccgcgatatg
     6241 ccggaggtcc cctgttgaga ggttgtcgat gacgcatacc tcatcccccc gggtgaccag
     6301 ggcatccacg aggtgtgacc cgatgaaccc ggcacctcct gtgacgaccg ccttcacgtt
     6361 gtttcccttc cccgtgtaat caatattaaa cgtctctctc ccatactaaa ggtgtggttt
     6421 ccagaatcga tgcgccacag ggcggccttg tctttttcaa gggggtgctc atgggcatgt
     6481 gcgacatcat ccccggggtc tcgggtggca cgattgcgct catcaccggg atttacgagc
     6541 gactgatatc ggcgattgga agtatcggga tatctccgct acgcgcgctg attcgcggca
     6601 ggagagagga ggcggttgat gcgatacacc gcatggaccc ggtgttcctt gtcgtgctca
     6661 tggcagggat tggcgccggc ttgcttgcaa tgtcaaggat cgtcctctcg gtcctgcagg
     6721 cattccccgc tgaaacatat gcgttttttt tcgggttgat cgtcgcctca tcggtcctca
     6781 tcgcgtaccg gatcgaacgg tgggatactg tcatcgcaac ggtatgggtg gggctgggcc
     6841 ttgccgccgg gtatttgatt gggggccttg gccagtcagg gctaggccat tcactgccga
     6901 tgctctttgt caccggtttt gttgccctgt gtgcgatgat tctcccgggc atatcaggtg
     6961 cgtacctgac ccttgtcatg aaccagtacg agtatctgct ctcggcaatc gccacgcttg
     7021 acatcgtccc tcttctcgcc tatatcgcgg gaggtgcggc aggcatcatc tcaatgagcc
     7081 gtatcctgaa gatcatcctt cgcacctacc acacgcagtc gctcgcgttc ctcacagggt
     7141 tgatgcttgg ctcgacaagg atgatgtacg accatattgt ggctgcaggc gggacaatcg
     7201 catctgtcct ggttgcaggg gttgcggggg tgctgctgat tgcgcttatc gaactggctc
     7261 tccgcctgtt ctcgaaacga agccccgatc ccgaataaca tgcgaccaga agaccggggg
     7321 agctcctgcc ggatcacaat gataaattcg ccgcgcgcaa agagatattg agatgtacgt
     7381 cggaatcgac cacgggacct cggcgatccg gttctctgca ggtgacggcc ggcggtttaa
     7441 agtcaggaga gaggacgcgg tatcgttccg gatacaggat ctctcccgcc tggccccgct
     7501 cgatgagatt gacgggatag ccctcactta ctcgatggga gacaatttct ctgcaatcac
     7561 tcccattcgg gatctcgaga accgaggcct tgtcagcagg gagggggccg ggaagcacac
     7621 ggggggtggg acccgggtct ttgaccaggt cgcagggagc agcatccctg ccgtggccat
     7681 accggggctc cacaggagtt cgcccaccga cccgcgcttc tctgtctatt cccaccaggc
     7741 cagcccggaa aagattggta ttgcgttcag ggtggtccag gacctcggcg gcgacgtggt
     7801 gatctctgat gtgagttcga acaccgtaag catcctggta cgggaccggc gactggtggg
     7861 agccctcgat gcgtgcatct ttgctcctgg caccgagcac ggtgcgcttg acgtggacgc
     7921 catccggagg gtggaccgcc gtgagatgag tgcgaacgag gcgttcctgc atgcaggggt
     7981 aaatcacacg ctctcaccgg aaaggagaat tcccgcactc gcgctttttt ctgcgatgga
     8041 atgcgcctcc ctcct
//
LOCUS       DAOQYR010000095         7889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_9654_length_7889_cov_8.043401, whole genome shotgun sequence.
ACCESSION   DAOQYR010000095 DAOQYR010000000
VERSION     DAOQYR010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 7889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7889
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9654_length_7889_cov_8.043401"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(338..1594)
                     /locus_tag="PKM87_09800"
     CDS             complement(338..1594)
                     /locus_tag="PKM87_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HNQ30400.1"
                     /translation="MASLFGMPLEKGRWVFVAIGLSINLCLGSIYAWSVFVEPLYDYY
                     TGSLGLAVTANEILMPFSVYLVFLAVSMPFTGKIIGKFGPRDVTILGGVLVGLGWLLS
                     SFSHSPLMLIVLYGVVGGIGGGVAYSVLVAVAACWFPDHRGLAVGITILGIGFSAFFT
                     ANIAGYLIEAFGVMNTFRIFGVMTIFLVFLLAQPLKFPDTGWIPLGWNPDGREPNVAG
                     HCECTRSRMLQTPSFYGLFACYFIGCLGGLMAISITKPIGTEVGVEAGLATFLVGFFS
                     IFNGFGRPFFGILTDLVTPRNATMVSFILIAAASILMWQIPTVPVYVIAFALLWGCLG
                     GWLAIAPTTTGNYFGTCDYPRCYGIVFLAYGAGAVAGPQLAAFIKTSTGTYAGVFPYL
                     LALSLVGLVVAFILLKPPAEGPCKRD"
     gene            1919..2197
                     /locus_tag="PKM87_09805"
     CDS             1919..2197
                     /locus_tag="PKM87_09805"
                     /inference="COORDINATES: protein motif:HMM:NF024694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HNQ30401.1"
                     /translation="MSRAHEASHHHFDIRLERGGVYISFAVPKGIPEKPGEKNFAIRT
                     GNHPLKYAGFEDAIPEGETGTGEVPIGDKDLYFMLEWTDDKIEVMMKG"
     gene            2408..2635
                     /locus_tag="PKM87_09810"
     CDS             2408..2635
                     /locus_tag="PKM87_09810"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HNQ30402.1"
                     /translation="MPEVNEPSVAKVIELIGSSPKGWEDAAANAVKEAAKTIRNIKGV
                     YLKECTAKVEQNRIVEYRSNVKISFVVDRES"
     gene            2939..3940
                     /locus_tag="PKM87_09815"
     CDS             2939..3940
                     /locus_tag="PKM87_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ30403.1"
                     /translation="MIGYEREIEAISRAIEQFDPSNPRHVAIIGEPMAGKTTVAGEIM
                     RRYGDCIHSVTLGGVMTQDAMPAFSSIQKDCILVDNCEFFATRQIGGFKFLDDFLKCQ
                     FSSTKLFITTWNSYAWRYLSAVRNIGTYYPLVVTLPAMDSSTLKQVILSNYPQREIRF
                     VENPPSEPASICTMVQRQARLPFSGREVSIPWFHLNTNAIRSRMQVGRETVVSPEDAV
                     FEKIRRIAQGNPGVAMQIFSGCLEDDAVSSEKIPEDECELSLDITESFVLTLILSMKS
                     LHYRDFSAMAGGGDVLDRALFSMRRQGLIFEDDGVYRISPARLNCIGNYLKKTRRVW"
     gene            3942..>4690
                     /locus_tag="PKM87_09820"
     CDS             3942..>4690
                     /locus_tag="PKM87_09820"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNQ30404.1"
                     /translation="MADSIFSDVTGELSLKTIDAGLILHLVAIVVVAYLLTTLLSYIL
                     SRFSERAGVYRQLVTLFIPLLKIFIYTVALYLFITALIEPSVTQLIAFAGLFGAVVGI
                     GLKDLFSDLAGGIVIILEKPFQIGDKVTMGNYYGEVKDIKLRSTRIQTPADELVSVPN
                     NTIFTQSVNSGNAGDIAMMIVIDLFIDSMSDAAHAAKILKEALVTSKYVIISEQYPYT
                     ILIEDFPCYTRVRAKGYVYDLRLEFEFKSEVT"
     assembly_gap    4691..4790
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4946..5422
                     /locus_tag="PKM87_09825"
     CDS             4946..5422
                     /locus_tag="PKM87_09825"
                     /inference="COORDINATES: protein motif:HMM:NF040157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5714 domain-containing protein"
                     /protein_id="HNQ30405.1"
                     /translation="MHGPEHHFLVPALLLTSYSLVTGEREALGGRLKRARERAAMVRG
                     GSCGTHGACRAAIGTGIFVSIITGSTPLSWREWMLSNLMTGRSLHEVALAGGPRCCKR
                     NTYIAILAAVSFVKEEWGIPIPVRRKLACEFSDLNRECRLAECPFYPGGDGGHQDA"
     gene            5684..>6414
                     /locus_tag="PKM87_09830"
     CDS             5684..>6414
                     /locus_tag="PKM87_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HNQ30406.1"
                     /translation="MVRKYSRPCIVVSRCIEFDHCRYDGSRIPSPAVDQLKSFVDFIP
                     VCPEVEIGLGIPRATVRIVRIEGVDHLIQPATGRDVTNDMTGFANRFLDGLPPVDGFI
                     LKGGSPTSGTSNVKVYPSAEKSMSIDRSPGFFAREVLKRYGDLPIEDELRLKHPRIRD
                     HFLSGIFTLAGFRQVEATGDPEALVTFHAENKLLLMASHQQLMREMGRFVADRRGKEP
                     EELWGRYRRMLCEALARVPRYTNHI"
     assembly_gap    6415..6514
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6515..>7889
                     /locus_tag="PKM87_09835"
     CDS             <6515..>7889
                     /locus_tag="PKM87_09835"
                     /inference="COORDINATES: protein motif:HMM:NF016158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HNQ30407.1"
                     /translation="LAEDPWFFLRHRFHAQKLVLHRAGMQAYRDYLEQEGYAVSSLES
                     AALPTSADVFAHLSKDGIKEVHLSDPVEHGLAKEIQAASAEQGIRLITYESPMFLCDR
                     EYLRRFYTDGGRFHLTDFYIAQRKRLNILLEADGKPVGKRWTFDTLNREPLPGDIPTP
                     PVWRPEWNEYVKNAAIYVREQFPGALGDPDDFSWPVTFDDAHRWFLDFLAHRLAQFGR
                     YEDAIHGNETFLFHSALSPLLNCGLLTPAEVVDATLEYARGHEIPIESLEGFIRQVTG
                     WREFVRAVYLIAGEREQASNVLMLSHRVPPCFWTATTGIEPVDTVIRRVHEHAYCHHI
                     ERLMVLGNIMLLAEFHPDQVYTWFSEFFIDAYDWVMVPNVYGMSQFADGGMMSSKPYV
                     SESRYILRMSNFRRGPWCDLWDALYWRFLYVHRDIFAHNPRMAPFIAYITRMPEERRS
                     VLIDRAG"
BASE COUNT         1853 a         2072 c         2029 g         1735 t
ORIGIN      
        1 atagaggtat ccggcttcac gcttgatctt ctcttttcca atttttgtac ccataattaa
       61 tcttgcgata ttgtcattta taagtgtttc gggtctccgc cctcgttttc cctccttgcg
      121 ggtatcacca cacggcctcc atatccctca ttgctacctc taagttaagt gagggcctgc
      181 tccttgcctc gctctatttc cgattccatc ccctggctta tttgcaccct ctgctccatt
      241 ttctggggaa aaacaggtct tattagggtt tcccgagtga gggtatggga gggagattgt
      301 ccattctgat cttcgcaatc tgtgaaaaat aatgatgtta atccctttta caggggcctt
      361 ctgcgggggg tttcagcagg ataaacgcaa ccactaaacc aacgagcgaa agcgcaagaa
      421 gataaggaaa cactcctgca taagtccctg tggacgtctt gatgaacgca gccagttgcg
      481 ggcccgcaac agccccggcg ccatacgcga ggaagactat tccgtagcac ctcggatagt
      541 cgcaggtgcc gaaataattc ccggtggtag tcggtgcgat ggccagccaa ccccccagac
      601 acccccataa cagcgcaaag gcaatgacat agaccggaac cgtcgggatc tgccacatca
      661 gaatggaggc tgctgcaatc aggataaacg ataccattgt cgcgttccgc ggggtaacca
      721 gatcggtgag tatcccgaag aagggacgcc cgaacccgtt aaagatcgag aaaaatccca
      781 cgagaaacgt ggcaagccct gcttcaactc ctacttcggt gccaatcggt ttggtgatcg
      841 agattgccat gaggcctccg aggcagccaa tgaaatagca agcaaagagt ccatagaaag
      901 agggggtttg gagcatcctg gaccgcgtgc attcacaatg gccagccacg ttgggttctc
      961 tgccgtccgg gttccatccc agggggatcc agccggtatc tggaaatttc aggggttgtg
     1021 caaggaggaa taccaggaag atagtcataa cgccaaagat cctgaacgtg ttcatgaccc
     1081 caaaggcctc gatcaggtac cctgcaatat tcgcggtaaa aaacgcggag aatccaatgc
     1141 ccaggatcgt aatgccgacc gcaagccccc ggtgatcggg aaaccagcat gcggcgacag
     1201 caaccagcac gctgtaggcc acgccaccgc cgatgccccc cacaacacca tacagaacga
     1261 tgagcatcag aggggaatgc gaaaacgaag agagaagcca gccgagacct accaggaccc
     1321 ctccaagaat cgttacatcc cgcggtccga attttccgat tatcttccct gtaaagggca
     1381 tggaaacggc taaaaatacg aggtacaccg agaatggcat caggatttca tttgccgtga
     1441 ctgccaggcc gagtgaaccg gtgtaataat catacaatgg ctcgacaaag acactccacg
     1501 cataaatcga acccaggcag aggttgatag agagacctat tgcaacgaac acccatctcc
     1561 ccttctccag gggcatcccg aagagtgaag ccatttcttt tatgtggttc ccttttatta
     1621 ccttgccact cctagaaaaa aaactgcatg gtttagccat ttcgtcctgg aatgcgcggg
     1681 aaatggggga gcgcgctgga aagattcgta tttcagtaaa tcctcttgca atcacctttt
     1741 catgcctggt gcatcagaat attccagcgc tctttatccc cgattggaag gccccctgca
     1801 tcaggttacg tccctacaag atcacaggat ttcctgatcg atacacatga aaaaggcatg
     1861 gccatcgggt ttgtaaaaga ttacgcatca gtccactctt tttcgtttct cagccacact
     1921 gtcaagggcg catgaggcca gtcatcacca cttcgacatc cggctggaga ggggcggcgt
     1981 gtatatctca tttgcggtcc cgaaaggtat ccccgaaaag cccggtgaaa agaattttgc
     2041 gatcaggacc gggaatcacc cgctgaaata tgcagggttc gaggacgcca tccccgaggg
     2101 ggagaccggg actggagagg tgcccatcgg ggataaggat ctttatttta tgcttgaatg
     2161 gacggacgac aagatcgagg tgatgatgaa ggggtgagaa cgtggagggg tggtcccttt
     2221 caggaacgat taaaaaaaag ccggaaataa ggactgggtg ctgctgaagg cgaaagatga
     2281 atgagagggg atgtagtggt caccggataa accttctgat aatcacctcc tcccatttcg
     2341 caattattct cttcatgcga aaacctcatg tgggggaggc aacgtaagga cattatggtg
     2401 gaaggagatg ccagaagtca atgagccctc tgtggcaaaa gtgatagagc tgattggcag
     2461 ttcgccgaaa ggttgggagg acgcggctgc caacgcggta aaagaagcag cgaagacaat
     2521 caggaacatc aagggtgtct accttaagga atgtacagcg aaagttgaac agaacagaat
     2581 tgtggaatac cgttccaacg tgaaaatctc atttgtagtg gaccgggaga gctaaacctc
     2641 tccccaggtt ttcgctattg gaatattttc atctcagtat cgcccctcct tcggaccggg
     2701 cctgacagct tccgcattct tccgggtgta aaataatcac tgaaagcgat gattcacgca
     2761 gtacctcctt caaatacaaa ttatcaggac tgatgtcgcc ctttgtggaa ttctttcaga
     2821 aggagggaac agtaaactgg cgatttatct ttatactcct ggagagaatt aggtgatagc
     2881 cgcatgagtg cttcaactgt cacttttcag ttacgagtct atgggagaag agagtaacgt
     2941 gatcggatac gaacgcgaaa ttgaggcgat ctcccgtgca atcgagcagt ttgatccttc
     3001 caacccgagg cacgtggcca tcatcggtga accaatggca ggcaaaacga cggtcgccgg
     3061 tgaaatcatg cgcaggtatg gcgattgtat acacagtgta accctcgggg gcgtcatgac
     3121 ccaagatgcg atgcccgcgt tttcatcaat ccagaaggat tgtatccttg ttgataactg
     3181 cgagtttttc gcgaccaggc agattggggg atttaaattc ctcgatgatt tcctgaaatg
     3241 ccagttttcc tctacaaaac tcttcatcac cacgtggaac agctatgcat ggcgatacct
     3301 ctccgcagtc aggaacatcg ggacctacta tcctctcgtg gtcacgcttc ccgctatgga
     3361 ctcctcaact ctcaaacagg tgatcctctc aaattatccc caaagggaga ttcggttcgt
     3421 tgaaaaccct ccctccgaac cggcgagcat ctgcaccatg gtccagcgcc aggctcgtct
     3481 gccattctcc gggagggagg tatccatccc ctggttccat ctgaatacca atgcaatacg
     3541 atcgcggatg caggtcggga gagagacggt ggtgtcaccg gaagatgcag tatttgaaaa
     3601 gatacgccgg attgcgcagg ggaatcccgg ggtcgcgatg cagatcttct ccggatgcct
     3661 ggaggatgac gcggtctcct ctgagaagat accagaagat gaatgtgaac tctctctgga
     3721 catcacagaa tcttttgtcc tcaccctcat tctgtccatg aagtccctgc actaccgcga
     3781 tttctctgcc atggcagggg gcggggatgt gcttgaccgg gccctgttca gcatgaggcg
     3841 gcaggggctc atatttgaag acgacggtgt gtaccgcata tctccggcga ggctcaactg
     3901 catcgggaat tacctgaaga agacccggcg ggtgtggtaa cgtggcagac tcgatctttt
     3961 ctgatgtgac gggtgagctc tcgctgaaaa ccattgatgc cggcctcatt cttcaccttg
     4021 ttgccatcgt tgttgtcgcg tatctcctca caaccctgct cagttacatt ctcagcaggt
     4081 tttcagagcg ggcaggtgta tatcggcagc tggtgaccct gttcatcccg ctcctgaaga
     4141 ttttcatcta tacagttgcc ctctacctct tcatcacggc tctcatcgag ccttcagtca
     4201 cgcagctgat tgcatttgca ggtctcttcg gggccgtggt cggcatcggg ctcaaggatc
     4261 tcttctcaga tctggcaggg ggcattgtga tcatccttga aaaacccttc cagatcggcg
     4321 acaaggtgac catggggaac tattacggag aagtgaagga tataaagctc cgctccacga
     4381 gaatccagac acctgccgat gaactcgtct cggtcccgaa taacaccatt tttacccaat
     4441 cagtcaacag tggcaatgcc ggcgacatcg cgatgatgat cgtcatcgat ctcttcattg
     4501 attcgatgtc tgacgctgca cacgccgcaa aaattctgaa agaggctctc gtgacatcga
     4561 agtatgtcat catatcagaa caatatccct acacgatact gatagaagat tttccatgct
     4621 atacccgcgt ccgtgcaaaa ggatatgtat acgatctcag actggaattt gaattcaagt
     4681 cggaagtgac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgtcactgaa
     4801 gtgagatgcg ttgagggtca tttcatctgc gatgactgcc accggcatcc tgcagaatac
     4861 ctcatagaga cgacgtgcct tggtacaaat attgtcgatc ccatcctcct tgcaatctcc
     4921 ctgatgcgac atccggcatt caaaaatgca cggacccgaa caccatttcc tcgtcccggc
     4981 cttactcctt acaagttatt cccttgtcac cggagagcgt gaagcgcttg ggggaaggct
     5041 gaagagggcc cgggaacggg ctgccatggt gagaggagga tcgtgcggga ctcatggggc
     5101 ctgcagagcg gcaatcggga cgggtatctt cgtgagcata attacgggat caacacccct
     5161 ctcctggaga gagtggatgc tctccaacct gatgactggc cgaagtctcc atgaggttgc
     5221 acttgccgga ggaccacggt gctgtaaaag aaacacttac atcgcaatcc tcgctgcggt
     5281 gtcctttgtc aaagaggaat ggggaatacc catccctgtc aggaggaaac ttgcctgcga
     5341 attcagcgat ctgaaccggg aatgcaggct ggcggaatgc ccgttttatc ccggaggtga
     5401 cggagggcat caggacgcat agagaacaca ccggggcttc ctgcggtcct ggaaatatat
     5461 attccacatc ctggtgcatg gatgcgtctt cacgatacac atgaaaagcc cgggaatcgg
     5521 ccaggagatc cccaggtttt cccgcaaaaa accttcaatt gaggatgctc tcttcgcaat
     5581 ctatgatgag acagactgtg acggccgatt cacttttaat agctgaacga ttccattcgc
     5641 attcatacct ttcctcatgg aaagaaaatg agggcgatct tgtatggtga ggaagtattc
     5701 acgaccctgt attgtcgtca gccgttgcat cgagtttgac cactgccgct atgatggttc
     5761 tcggatacct tctcctgcag tggaccaatt gaaatccttt gttgacttca taccggtgtg
     5821 ccccgaggtg gagatcgggc tcggcatccc gagggcaacg gtgagaatcg tgcggattga
     5881 gggggtcgac caccttatcc agcctgcgac cgggcgggat gtgacgaatg acatgaccgg
     5941 cttcgccaac cggtttctgg acggcctccc ccctgttgac gggttcatcc tgaagggtgg
     6001 atcgcccacc tccgggacaa gtaacgtcaa ggtatacccg tccgcggaaa aatccatgtc
     6061 aatcgacagg tcccccggtt tttttgcccg ggaggtgctg aagaggtatg gtgatctccc
     6121 catcgaggac gaactgcgcc tgaaacaccc acgtatccgc gatcatttcc tctcggggat
     6181 atttaccctt gcaggattcc gccaggtcga agcgacgggt gaccccgaag cgctggtcac
     6241 gttccatgca gagaataaac tcctgctgat ggcctctcac cagcagctca tgcgcgagat
     6301 ggggaggttc gttgccgaca ggagaggaaa ggagccggaa gaactatggg gccggtaccg
     6361 ccggatgctc tgtgaagcgc tcgcccgcgt tccccgctac accaaccata tcaannnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgctgg cggaagaccc ctggttcttc
     6541 ctgagacacc ggtttcatgc ccagaaactc gtgctccacc gtgcgggcat gcaggcatac
     6601 cgcgactacc ttgaacagga ggggtacgcg gtctcctctc tcgaatcggc agctcttccc
     6661 acaagtgcag acgttttcgc gcacctctcg aaagacggga taaaagaagt ccacctctcc
     6721 gatcctgtcg aacacggcct cgcaaaggag atacaggctg caagtgctga gcaggggata
     6781 cgactcatca cctacgaaag cccgatgttc ctctgcgacc gggagtacct ccgccggttc
     6841 tacacggatg gcgggcgatt ccacctgacc gacttctata tcgcacagcg aaagcgcctc
     6901 aacatcctgc tcgaggcgga tggaaaaccg gtggggaagc gctggacatt cgatacgctc
     6961 aaccgggaac ctttaccggg ggatatccct acacccccgg tctggcgccc tgaatggaac
     7021 gaatacgtga aaaacgcagc tatttacgtg agggaacaat ttccaggtgc ccttggagat
     7081 ccggacgact tctcctggcc ggtcacgttc gacgacgcac atcgctggtt tctggatttt
     7141 ctcgcacacc gcctggcgca gttcgggcga tacgaggatg ccatccatgg caacgagaca
     7201 ttcctcttcc actccgcact cagtcctctc ctcaactgcg ggttgctcac ccctgcagag
     7261 gtggtggacg ccacgcttga gtatgcccgt gggcacgaga tcccaatcga gtccctcgag
     7321 gggttcatcc gccaggtgac agggtggagg gagtttgtca gggcagtcta cctgatcgcc
     7381 ggagagcgcg agcaggcctc aaacgtgctc atgctttccc acagggtgcc tccctgtttc
     7441 tggacagcta ccaccgggat cgaacccgtg gataccgtca tccgtcgcgt ccatgaacac
     7501 gcctactgtc accatataga gcgcctgatg gtgctcggca atatcatgct cctcgctgag
     7561 ttccatcccg accaggtcta tacctggttc tcggagttct tcatcgatgc ctatgactgg
     7621 gtaatggtcc ccaacgtcta tggcatgagc cagtttgcag atggcgggat gatgagttcc
     7681 aagccctacg tatccgagtc ccggtacatc cttcggatga gcaattttag gagaggcccc
     7741 tggtgcgacc tctgggacgc gctctactgg cgttttctgt atgtccaccg cgatattttt
     7801 gcacacaacc cgcggatggc cccctttatc gcctatatca cccggatgcc cgaggagagg
     7861 cgatcggttt tgatcgacag ggccggaca
//
LOCUS       DAOQYR010000096         7889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_9655_length_7889_cov_7.758361, whole genome shotgun sequence.
ACCESSION   DAOQYR010000096 DAOQYR010000000
VERSION     DAOQYR010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 7889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7889
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9655_length_7889_cov_7.758361"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="PKM87_09840"
     CDS             complement(<1..1222)
                     /locus_tag="PKM87_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNQ30408.1"
                     /translation="MITLPFLILFPAAIALLLLLVPGDSWRRLIVKAGALIIGIASLY
                     LLFITPEAGPEFFPVAFEPVSNLMFLAEMVIAAFLIYLGVKYKKYLAILLVIAQAALL
                     VYYESMGLTRIHTVPNLFIDQFSLIMALIIGIIGSLICVYALGYMATYQEHHPEVKDR
                     RRTFFFLMFFFLSSMFGLVFSDYLPWVFFFWELTTLCSFLLIGYSGTGEAVNNAFLAL
                     NMNLLGGIAFVGALIYLAFVSPGGELLDISTLIQSGHMVALVPAVLIAFAGITKAALM
                     PFSAWLVGAMVAPTPVSALLHSSTMVKAGVYVIVRFAPVLTGTMEGLIIGLVGGFTFL
                     LASAIAISQSNAKKVLAYSTIANLGLIVACAGVGTYKLVWAAILLIIFHAIAKSLLFL
                     CVGTVEHRIGSKDIE"
     gene            1497..1775
                     /locus_tag="PKM87_09845"
     CDS             1497..1775
                     /locus_tag="PKM87_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30409.1"
                     /translation="MERVVERIRKQMGADWTHFSQEDQNALKYVIGEVWVYKEREFWD
                     MVQYPRITAISVFDIIAIGRKSLSHEIDTQRTVEEATAILLPDEGKEA"
     gene            1923..2057
                     /locus_tag="PKM87_09850"
     CDS             1923..2057
                     /locus_tag="PKM87_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30410.1"
                     /translation="MKSYRHLRHVLTTSRAGHGFCREADQRGLIAIYERIERKSSRRD
                     "
     gene            2288..2623
                     /locus_tag="PKM87_09855"
     CDS             2288..2623
                     /locus_tag="PKM87_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30411.1"
                     /translation="MGMPEVTNDERGRRVFKIHQEMAVEKAIEKIRLNLGADWKIYSA
                     HDIELLKYMLGECWGSLDRRKWEGFTFTRLTKDGVDAIIGVGKEVKREGRLESDAIQE
                     VIGLLQKIA"
     gene            complement(2925..3572)
                     /locus_tag="PKM87_09860"
     CDS             complement(2925..3572)
                     /locus_tag="PKM87_09860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNQ30412.1"
                     /translation="MEQINPTRMELIKKNAQIKLAEQGRDLLREKMDALIQEFFNVME
                     SVSQSRSELEKLSEVAQHALLIAEAIDDPVTLKSASFATRRGITLDIKGKNVMGVPVP
                     IIEKKRFVKSLFERGYSILGVSGRIDEAAEKYEAELDLIITLAETETSLRRLGSEIQM
                     TRRRVNALEQVLIPELKRQAKYIEITIDERDREDLYRLKKVKKIIERKKRETIDT"
     gene            complement(3572..4972)
                     /locus_tag="PKM87_09865"
     CDS             complement(3572..4972)
                     /locus_tag="PKM87_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNQ30413.1"
                     /translation="MIRVPLFTKEARAINYVSGPLIFIQNVKGVSYGEIVRIILPTGE
                     ERTGQVLDISKDIAVTQVYEGTSGIDNKETRVRFTGEPPKVKVSIEMLGRIFNGIGKP
                     RDGGPEIIEESNLDINGMPINPSARDKPADFIQTGMSTIDGLNTLVRGQKLPIFSGAG
                     LPANKLAAQIARQARVLGEEEKFAVIFVAMGITYKEASFFMRDFEKTGALERVVFFMN
                     LADDPTIERIATPRCALTAAEFLAYEHDLHVLVILTDMINYCEALREISTAREEVPGR
                     RGYPGYMYTDLASIYERAGRVKGRKGSITQIPILTMPDDDITHPVPDLTGYITEGQIV
                     LSRDLFRRGADPPIDPLPCLSRLMNLGIGPGKTREDHRNVADQLYASYAYGRDLRRLV
                     AIVGEEALTELDKRYLKFADEFERHFITQGDENRSIERTLEIAWQLFTLLPEEELKRI
                     KREFIQKYHPAAQKGM"
     gene            complement(4969..6708)
                     /locus_tag="PKM87_09870"
     CDS             complement(4969..6708)
                     /locus_tag="PKM87_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNQ30414.1"
                     /translation="MIVGAISRISGPVVLSEGMRGSRMYDVVKVGTQQLNGEIIRLDR
                     DQAVIQVYEDTTGLQVGEHVSNTGLPLSVELGPGLLSSIYDGIQRPLPLLAEKSGSFI
                     SRGISVPGISHEKRWRFSPRVKTGVRVSGGDVIGTAHEYHLEHRIMVPPFVSGMVRDI
                     YEGEFTIDEPVCILEDGRQITMMQTWPVRKGRPYQRKMDPDTLLVTGQRVFDTLFPVT
                     KGGTAMIPGGFGTGKTVAEQTLAKWSDTQIVVYIGCGERGNEMTDVLTEFPELLDPKT
                     KLPLIQRTVLIANTSNMPVAAREASIYTGITIAEYYRDMGYDVALMADSTSRWGEALR
                     EVSGRLEEMPGEEGYPAYLATRLAAFYERAGRVVCLGSDERKGSVTVVGAVSPPGGDF
                     SEPITQNTLRIAGTFWALDTSLAYRRHFPSVNWIKSYSLYLDQIQDWYTASGFSDWKE
                     LRARAMYLLQKEVELQEIVQLVGPDALPESEKAILEITRMIREDFLQQSAYHDIDSFC
                     SLEKQYWMLKVILFFYDRLNQSMGKGISLSKILKLPVKAEIGRMKELTEIEKIKDLVD
                     QIDGSLGSLEVER"
     gene            complement(6713..7033)
                     /locus_tag="PKM87_09875"
     CDS             complement(6713..7033)
                     /locus_tag="PKM87_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HNQ30415.1"
                     /translation="MYKFIVVTDPDTAPGFRLAGVDVMEIHDSEEVHALLPSLLLKDD
                     TGIIAINEDYMMSLEEKMMEKIEKSYRPIIIPIPSRAKRLDRTSYIEGLLRRAIGYNI
                     VLRK"
     gene            complement(7026..>7889)
                     /locus_tag="PKM87_09880"
     CDS             complement(7026..>7889)
                     /locus_tag="PKM87_09880"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HNQ30416.1"
                     /translation="AIRKDLVKTFRTVLNLIRGGETESYLTIILNRWDVQNIKTILRG
                     KKIQENPSEILECLIPAGELDEAALTELVKQPDVKAVIDLLATWGIIYSRPLTLNFKE
                     FSETGDLLVLEYALDTFYYQDAISRLKSGESDDARILKNILVTEIDINNIKTVLRSIR
                     DRIDIEEAKRYFVKGSPDFDIERLTSMMKAGTIDGALKQLEHTPYQFITTIPQEYIVA
                     EKISAFEKELERYLVKKAVSLFIGDPLSIAIPIAYIWAKYTEVTNIRVIARCKIIEVP
                     EKELREALISV"
BASE COUNT         1817 a         2041 c         2123 g         1908 t
ORIGIN      
        1 cctcgatgtc cttgctgccg atgcgatgct cgactgtgcc cacgcagagg aagagaagcg
       61 acttggcgat ggcatggaag atgatcaaga ggatcgccgc ccagacaagc ttgtaggtcc
      121 ccacccccgc acaagccacg atgaggccga ggttcgcgat ggtggagtat gcgagcacct
      181 ttttcgcatt gctctgggag atggcaattg cggatgcaag caggaaggtg aacccaccca
      241 caaggccgat aatcagccct tccatcgtcc cggtcagaac aggcgcgaac ctcacgatta
      301 cgtagacccc tgccttgacc attgtgctcg agtgcaggag ggcggagacc ggggttggtg
      361 cgaccatcgc ccctacgagc catgcagaga acggcatcag cgctgccttt gtgatcccgg
      421 caaaggcgat gagtacggcg ggcacaaggg cgaccatgtg gccggactgg atgagagtgc
      481 tgatatccag caactcgcca ccggggctga caaaggcgag gtaaatgagt gcgccgacaa
      541 acgctattcc ccccaggagg ttcatgttca gtgcaaggaa cgcattgttg actgcttcac
      601 cagtgcccga gtacccgatg aggaggaagg aacagagcgt cgtgagctcc cagaagaaga
      661 agacccaggg gagataatcg gagaagacca gcccgaacat ggaggagagg aagaagaaca
      721 tgaggaagaa gaacgtcctt ctccggtcct tcacctcggg gtggtgctcc tgataggtgg
      781 ccatgtatcc cagggcgtat acgcagatga ggcttccgat gatgccgatg atcagggcca
      841 tgatcaatga gaactggtcg ataaagagat ttggcacggt atgtatccgt gttaagccca
      901 tggattcgta gtagaccagc agtgccgcct gcgcgatgac caggaggatt gcgaggtatt
      961 tcttgtattt taccccgagg tagatcagga acgcggcaat gaccatctcc gccaggaaca
     1021 tcaggttcga gacaggttcg aatgcgaccg ggaaaaattc cggtcctgcc tcgggcgtga
     1081 tgaacaggag gtagagggaa gcaatgccga tgataagcgc gcctgccttc acgatcagcc
     1141 tcctccaaga atcgccaggg acgagcagga gaaggagagc tattgcggca ggaaagagta
     1201 tcaggaatgg cagtgttatc ataaactcgc cctcgaatgg gtagaagtct ggatttctgt
     1261 cattattaat cattccaatt tgacgcaaat gaaatgttac aggcgatatt tgatgatctc
     1321 cggaagactg gactcgcatc tcgcgagaat atcccactaa aaaaattccc tggcaaaacc
     1381 tttttaatcc cggggtatat ctccgcatat ccacagggag gggcggatag cagatgccgg
     1441 atgtatccag gacagagatt gggaggcgga tgtactcgct gcaaaaggag aagaacgtgg
     1501 agcgggtcgt ggagaggatc aggaagcaga tgggggctga ttggacacac ttttcacagg
     1561 aagaccagaa tgcattgaag tatgtgatag gggaagtgtg ggtctataag gagcgagagt
     1621 tctgggatat ggtccagtat ccccgcatca cagctatttc ggtctttgac atcatagcca
     1681 ttggaagaaa gtctctttcg catgagatag atacgcagag aactgttgaa gaggcgactg
     1741 ccatcctgct cccggatgag gggaaggaag cgtgatcatt gccggggaaa aagaggagac
     1801 cctgtgcagt gcatgagcgc aatgcaagga tttattgtta aagtccgcaa aaatagctga
     1861 tgttgtatcc cgggatttat cgcgttcccg ggacatccag caccgataag gaggttaatt
     1921 gcatgaaatc gtaccgccac cttcggcatg tgctgacaac atcacgtgcc gggcacgggt
     1981 tctgccggga ggccgatcag cgggggctga tcgccattta cgagaggatc gagaggaaaa
     2041 gttcccgccg ggattgaact acccgcatca cgtgggaagc tggatagagg ggaaaggaat
     2101 ctctcttcgc gagggagtga atgtcacaca cggaaggaga gacgtcggcg tccatcttcc
     2161 tttgcaccgt tcggacttta cgatatttgt acttttccgt tcttttttcc ctcgaaaatt
     2221 cgcctcacca cccgggggaa gtgtccgaaa agatatatat tctccgcagg gactctaaag
     2281 tgagctggtg ggtatgcctg aagtgaccaa tgatgagcgg ggccggagag tgttcaagat
     2341 ccatcaagag atggcagtcg agaaggccat cgagaagata cgactgaacc tgggagcgga
     2401 ctggaagatc tattcagcac acgatattga gcttctcaag tatatgctgg gtgagtgctg
     2461 gggatccctg gatcgcagaa aatgggaagg attcacgttt acaagactca caaaagatgg
     2521 tgtcgatgct atcatcgggg ttggaaaaga ggtgaagcgg gaaggccggc tggagagtga
     2581 tgcgatacag gaagtaatcg ggttgttaca aaagattgcg tgaacattag attaataacc
     2641 cgggccttaa ttgtgtaatg ctgttcattc actacatatt ttccttctta tctcttccct
     2701 gcccgcgtta tgttgtcgca atccgtgaag gtgatgtccc aggttgtgat gtgggccatg
     2761 aggaaaaaag gtcgtcctcc tgaaacctgg acgaaccaat ctctcacaaa gacaaaatac
     2821 cgctatggat cctgatgtgg atttccgaac ggaaaaaccg taatcctgct ggattaaaga
     2881 gtcccatagc gacgagggag atgattcata tgggtaccat gccttcaggt atctattgtc
     2941 tctcgtttct ttctctcgat gatctttttt acttttttca accggtaaag gtcttctcgg
     3001 tccctttcat cgatcgtaat ctcgatatat tttgcctgtc gcttcaattc tggaatgagt
     3061 acctgttcaa gtgcatttac gcgccttcgc gtcatctgga tctcgctacc gagccttcgg
     3121 agagatgttt cagtctctgc gagagtgata atgagatcca gctccgcttc gtatttctct
     3181 gccgcttcgt caatccgtcc gctgaccccc aggatgctgt acccccgttc gaagaggctc
     3241 ttcacaaaac gcttcttttc gatgatagga acaggcacac ccatgacatt ctttcccttg
     3301 atatcaaggg tgatacctct cctggtggca aatgaggcag acttcaatgt cacggggtcg
     3361 tctattgcct ctgcaatgag cagggcatgc tgagcaacct cggagagctt ttcaagctcg
     3421 cttcgcgact gcgataccga ctccatgaca ttgaaaaatt cctggatgag cgcatccatc
     3481 ttttcacgga gaagatcgcg cccctgctct gcaagcttga tctgtgcgtt cttcttgatg
     3541 agttccatcc gggtcgggtt aatctgttcc atcacatccc cttttgagca gcaggatgat
     3601 atttctgaat gaattcacgt ttaatccgtt tcaattcctc ttcagggagc agggtgaaca
     3661 actgccaggc tatctcaagc gtcctctcga tgctccggtt ctcgtctccc tgcgtgatga
     3721 aatggcgctc gaactcgtcc gcgaatttca ggtatctctt gtcaagctcg gtgagcgcct
     3781 cttcccccac aatcgcgacg agcctccgca gatccctccc ataggcatat gatgcataga
     3841 gctgatccgc gacattccga tgatcctctc tcgtctttcc aggtccgata ccaagattca
     3901 tcaggcgtga gaggcatggg agtgggtcga ttggaggatc ggccccgcgg cgaaagagat
     3961 cgcgtgagag gacgatctgt ccctcggtaa tatagccggt aaggtcaggg accgggtggg
     4021 tgatatcgtc atccggcatc gtgaggattg ggatctgggt gatggatcct ttccggcctt
     4081 tcaccctccc tgcccgctcg tagatggatg cgaggtcggt gtacatatac ccgggatacc
     4141 cccttcggcc tggcacctcc tcccgtgccg tggagatctc tcgcaatgcc tcgcagtagt
     4201 tgatcatgtc agtgaggatc acaagcacat gaaggtcgtg ttcataggcg agaaattcag
     4261 cggcagtgag ggcacaacgg ggggtggcga tcctctcgat agtgggatcg tctgcaaggt
     4321 tcatgaagaa caccaccctc tctaatgcac ctgtcttttc aaaatccctc atgaagaagg
     4381 aggcctcctt gtatgtgatg cccatggcga caaagatgac cgcgaacttc tcctcttccc
     4441 cgaggaccct ggcctgccgc gcaatctggg cggcaagctt attggccgga aggcccgcac
     4501 ccgaaaagat gggcagtttc tggccgcgga caagcgtgtt caggccatca atcgttgaca
     4561 tgccggtctg gatgaaatca gcgggtttgt ccctggcgga tggatttatg ggcattccgt
     4621 tgatgtccag atttgattct tcaatgatct cgggtcctcc gtcgcggggt tttcctatgc
     4681 cattgaagat ccgtcccagc atctcgattg agacttttac cttcgggggt tctccggtga
     4741 accggacccg tgtctccttg ttgtctatac cgctggtccc ttcgtatacc tgggtgacgg
     4801 cgatatcctt cgatatatca aggacctgcc cggtgcgttc ttcgcctgtg gggaggatga
     4861 tccggacgat ctcgccgtac gagacgcctt tcacattttg aataaaaatc agagggcctg
     4921 aaacatagtt gattgcccgg gcctcttttg taaagagcgg cacccggatc atcgctccac
     4981 ctccagagaa ccaagagagc cgtcgatctg atcgacaagg tccttgatct tttcgatttc
     5041 ggtgagctcc ttcattcgcc caatctctgc tttcacgggc agtttcagga ttttgctgag
     5101 agaaatcccc tttcccattg actggttcaa ccggtcataa aagaagagta tcaccttgag
     5161 catccaatac tgcttctcaa gcgagcagaa cgaatcgata tcgtggtatg cactctgctg
     5221 gagaaagtcc tcgcggatca tgcgtgtgat ctcaagaatt gccttctcgc tctccggtaa
     5281 tgcatctggg cccacgagct gcacgatctc ctgcagttcg acttctttct ggaggagata
     5341 cattgcccgc gccctgagct ccttccagtc ggaaaaccca ctcgcggtat accaatcctg
     5401 gatctggtcc aggtaaaggg aatagctctt tatccagttc acggagggga agtgccgcct
     5461 gtacgcaagg ctcgtgtcga gtgcccagaa tgttccggca atgcgaaggg tgttctgggt
     5521 tattggttcg gagaaatctc cgccgggagg agaaacggcc ccgaccaccg tgaccgaacc
     5581 cttccgttcg tcagagccga gacagaccac gcgacccgcc ctctcgtaga atgcagcgag
     5641 tcttgtcgca aggtatgcgg ggtacccttc ttcccctggc atctcttcaa gtctgcctga
     5701 tacttcacgc aatgcctcgc cccaccggga ggtggagtcg gccatgagcg cgacatcgta
     5761 ccccatatca cggtaatact cggcaatggt gattccggta tagatagaag cctcccgtgc
     5821 agcgaccggc atatttgagg tattggcaat aaggactgtc ctctgtatca atgggagttt
     5881 ggtctttggg tcaagcagtt cgggaaattc ggtgagcaca tccgtcatct cgttccccct
     5941 ctcaccgcaa ccgatataga ccacgatctg ggtatccgac cacttggcaa gcgtctgctc
     6001 tgcgacagtc ttcccggtac cgaatccccc cggaatcatc gccgtccccc ctttcgttac
     6061 ggggaacagc gtatcaaaga cacgctgacc ggtaaccagc aacgtatcgg ggtccatctt
     6121 gcgctggtat ggtctccctt tccgtaccgg ccaggtctgc atcattgtga tctgtcttcc
     6181 gtcttccaga atacagacgg gttcgtcgat ggtaaactcc ccctcataga tgtcccttac
     6241 catccctgag acaaacggcg gcaccatgat gcgatgttca aggtggtatt catgtgccgt
     6301 tccaatcaca tcgcctccgg aaacccgcac ccccgtcttt acccgtggtg aaaatctcca
     6361 cctcttctcg tgcgagatcc cggggacaga gataccccgc gatatgaaac tcccgctctt
     6421 ctccgcaagg agcgggagag gcctctggat accgtcgtaa atggaagaga ggaggcccgg
     6481 tccaagctcg actgagaggg ggagtcctgt attggatacg tgttcaccca cctgcaggcc
     6541 cgtggtgtcc tcatacacct ggatgacggc ctgatcgcga tcgagccgta tgatctcgcc
     6601 gttcagctgc tgtgttccga ccttcaccac gtcatacatg cgggatcccc gcatgccctc
     6661 ggaaaggaca acgggacccg aaattcttga aatagcaccg acaatcatac agtcacttcc
     6721 tcagcacgat attgtaccca atcgctcttc gcagaagtcc ctcgatgtaa ctggtccggt
     6781 ctagcctttt tgcgcgggat gggattggga tgatgatggg ccgataggac ttctcgattt
     6841 tttccatcat cttctcttca agcgacatca tatagtcctc gttgatcgcg atgatgccgg
     6901 tatcgtcctt gagaagaagg gatgggagca atgcatgaac ctcttcggaa tcatggatct
     6961 ccattacatc cacgcctgca aggcgaaatc ccggtgcggt atcgggatcc gtcaccacga
     7021 tgaacttata cactgataag cgcctccctg agttcctttt ccggcacttc gatgattttg
     7081 cagcgggcaa tcacgcggat gttcgtcact tcggtatact ttgcccagat atacgcgatc
     7141 ggtattgcga ttgagagggg atctccaatg aagaggctta ctgccttttt tacgagatac
     7201 cgttccagtt ccttttcaaa tgccgagatc ttttctgcaa caatgtattc ctgggggatg
     7261 gtggtgatga actggtaggg agtatgctcg agctgtttca gcgccccatc gatggtccct
     7321 gccttcatca tcgaggtgag cctttcgata tcaaaatcag ggctaccttt tacgaaatag
     7381 cgttttgcct cctcaatatc gatacgatcc ctaatcgaac gaagcactgt cttgatgttg
     7441 ttgatatcta tctcggtcac gaggatattt ttgaggatgc gtgcatcatc cgattctccg
     7501 gatttcaacc ttgagatggc atcctgataa tagaacgtgt cgagtgcata ttcaaggacc
     7561 agcaagtctc ctgtctcact gaattccttg aaattaagcg tcagggggcg cgagtagata
     7621 atgccccacg tcgcaagaag atcgatcacc gccttcacat cgggctgttt cacgagttcg
     7681 gtgagggctg cttcgtcgag ttctccagct gggatcaggc attccagaat ttctgatgga
     7741 ttttcctgga tcttcttccc ccttaaaata gtcttgatat tctggacgtc ccaccggttg
     7801 aggatgatgg tgaggtagga ctctgtctct cctcctcgta tcagattgag gaccgttctg
     7861 aatgtcttga caagatcttt ccgaatcgc
//
LOCUS       DAOQYR010000097         7734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_9841_length_7734_cov_8.932153, whole genome shotgun sequence.
ACCESSION   DAOQYR010000097 DAOQYR010000000
VERSION     DAOQYR010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 7734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7734
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9841_length_7734_cov_8.932153"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..780
                     /locus_tag="PKM87_09885"
     CDS             304..780
                     /locus_tag="PKM87_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNQ30417.1"
                     /translation="MEIVKIPRMDKKEYDSLIEKGYVCRIAFQGEKYPYIAPFLYVFD
                     GKFLYFLSTKYGKKLEYFRKSPYVAVEVEKYTRDLSSYTFVSMQGYLEEVTDSIEKKL
                     IRQEFVDLIKERRLSKNILAALGHSPQDPPEAIAMEERSLVWKLTGVMDMVALKNL"
     gene            complement(832..2946)
                     /locus_tag="PKM87_09890"
     CDS             complement(832..2946)
                     /locus_tag="PKM87_09890"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNQ30418.1"
                     /translation="MTDPDAGERDSSNPANRLVHEASPYLQQHAHNPVDWYPWGEEAF
                     SRARAEGKPIFLSIGYSTCHWCHVMERESFSDREVAALLNAHFICIKVDREERPDLDQ
                     LYMDAAIALTGSGGWPLNVVLTPDLHPFFAATYLPRENRMGMPGLLEVLPRLAEFWEE
                     KQEEALESAGLLARAIAADRKARPGGRVRKDTADRMLQDLLLQYDSLNGGFGPAPKFP
                     MPHLHLFLLRYWKWTGSGKALGMAEKTLLSMARGGIYDHLGGGFHRYSTDARWLVPHF
                     EKMLYDQALAAMAFTEAWLATGNQEFWEVASGCLDYACSRLSAPDGGFFSAEDADSEG
                     VEGKYYLWTHDEIASLLDPEDARVAFLVFPVSRTGNYLDPHTGRYGRENVLHRTHSEK
                     EAARALSLSPGEVSERIERARATLLRAREMRTRPFCDEKVLADWNGLAIAALAMAGRA
                     SGRSRYIEAAEKAATFVLSRMRMDDGGLYHRFRDGSAGIPGLSADYAAMVWGLLELFV
                     ATRDPGHLESALVLEDYHDAHFWDKRSGGYFTSPESAIDLFARRKEFTDGAIPSPNSI
                     SFCNLVRIGLITQEERFGRRADALSRLYSPLVERSPASCGLFMAGFTLSAGPATRVVV
                     TGKGTDPAFRGMNAFLDRHYLPFTIPVSLEQGKQYELLFRLAPSIASHRFAGEAAVAH
                     VCTMDSCAPPAYSVEDLSLRLG"
     gene            complement(2939..3064)
                     /locus_tag="PKM87_09895"
     CDS             complement(2939..3064)
                     /locus_tag="PKM87_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30419.1"
                     /translation="MGLVFLYAAVCFFPAFRATRREPLDEERKRESLRPGSGAGD"
     gene            3190..5856
                     /gene="mutS"
                     /locus_tag="PKM87_09900"
     CDS             3190..5856
                     /gene="mutS"
                     /locus_tag="PKM87_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNQ30420.1"
                     /translation="MEKGSRSGGAGAESRLSPAMAQYQEIKSKYPDAILLFHIGDFYE
                     TFNEDAETVSRQLDIVLTSRSRDRSGNRIPLAGVPCHAVEGYIARLIAKGYKVAVCDQ
                     VEDARAAKGIVKREVVRVVTPGMVMDETLIPSPGAHYLLAISPNAKEGEAGLAFLDIT
                     TGEFSVVTVPLEHGFSALQGEVARNSPAECLLPADAPPEIAEFLSRGGLAVSRGAADI
                     FSPEKGRDALCRHFGVASIEGFGLARDSPAEQAAGAALLYAKETQRSELPQINALSVR
                     HSGESCLLDAITLRNLEIVQGIRDRGKEGTLISVLDRTATPMGSRLLRQWLCEPLVNV
                     GAINTRLDAVGHLVRNAAARMEASVTLRRCADIGRIAGRIAFGNATPRDLKSLERSLA
                     TIPQLKAILRYGQGAEIPAFIQDACGMLSDLSWVGETIGAAIVDDPPAVVRNGGIIRD
                     GFSPELDSLRGISTSGKEWILDLQQRERERTGIKSLKIAYNSVFGYYIEVTRPNLSLV
                     PPEYQRKQTTSNGERFTIHELKEKEAVISSADERQLSLEQEVYSGLLERLNGAVPMMQ
                     EIARGIALLDVAVSLAEVAESGHYCRPVVDESTHLLVREGRHPVVEVGMAGRFVPNDL
                     ELSSEGDQILIITGANMAGKSTYMRAAALVAVMAQAGSFVPAAYARIGVVDRVFTRVG
                     AFDDLASGQSTFLVEMLELANILNNVTGRSLVILDEIGRGTSTLDGLCIARAVLEFLH
                     GKKSQGPRTLFATHFHELVTVEGEFSRVKNFHFAAKDTGREVVFLRKIIPGATDKSYG
                     IHVAALAGVPRRVTDRARVLLEEMAGAEGRSSGPRVKRYTQMLLPEIPAVDAPAHPVL
                     ETLKNMNLDDMTPLSALSLLYDLQKRAREGGR"
     gene            5860..7653
                     /gene="mutL"
                     /locus_tag="PKM87_09905"
     CDS             5860..7653
                     /gene="mutL"
                     /locus_tag="PKM87_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNQ30421.1"
                     /translation="MAIGREPGEIQVLDQQTVSRIAAGEVVERPASVIKELVENALDA
                     GATRVEVEITSRKGIITGIRVTDNGSGMTPKDADLACVRHATSKIRTLSDLLLVQSLG
                     FRGEALASIAAVSRLSLITRKAGTALSGVRIVNEGGEVIERGECGCPEGTTVEVEEIF
                     FNTPARRKFLRSLPAEMAYITGILERIILSRPDVSFRVLHNRKVLMSGPGGSVHDAAL
                     HLFGTGVARALIPLDFEGSFIRVGGVISHPSLSRQNPYQVFLSINRRPVQSRALSLAV
                     KAGYGTLLPGDRFPVAILDITIDPHLVDVNVHPTKREVRLSREREVKGEISRAVETAL
                     KDMDLTFEARESGKASTQATISPASGSPAYHIDEPSLATVAEPGRSIRADTDRRLRQT
                     ELGLSAGTAGQSPVPDLEVLGTLDNTYILACPRGGEDLILIDQHAAHERILYEQVLDT
                     GQREPECQELIVPVPLHLSPREAAILPALIPLLEEIGFSIEKFGRGTYAVRAIPIVLG
                     KQIGQEGVREVVDALLSGDVRQDLSEKERILKVIACRGAIKGGTPLTMEQCRSLIAQL
                     RRTEHPFSCPHGRPTMVTFKRRELDGLFFRT"
BASE COUNT         1604 a         2287 c         2366 g         1477 t
ORIGIN      
        1 gattaaagag gtctcttttg tcccttatgg aagtattgtt tggcaacatg cgaaacaggg
       61 gagaaaaatt ttcctgtcaa ggagaggtat ccgcttttgg cagcatacaa atgcgcagga
      121 ataatcttat acagggctgt tcatctccca ttcgggcagc cgcccccgcc gtcctgcgtg
      181 ccggccgccc atggccccga agtgccccgt tgtaaaatca aggcaatccc tggcatcttt
      241 ttcgcaaacg catgaataca caaactataa gagtgaggga tcgtaatgct gaatgcggta
      301 gttatggaga tagtgaagat ccccaggatg gataaaaagg agtacgactc cctgatagag
      361 aaaggctatg tctgccggat agccttccag ggggagaaat acccttatat tgcacctttt
      421 ctctacgtat ttgacggtaa gttcctctat ttcctctcca caaagtatgg aaagaagctc
      481 gagtacttca ggaaaagccc ttacgtcgca gttgaggtgg agaagtacac ccgcgacctc
      541 tccagttata cctttgtcag catgcagggg tacctcgagg aggtgaccga ttcgatcgag
      601 aagaagctga tccgccagga gttcgtcgac ctgataaagg aacggcgcct ctccaaaaac
      661 atcctcgcgg ccctcgggca ttcgccccag gacccgcccg aggcaattgc catggaggag
      721 agatccctcg tctggaaact gacaggcgta atggacatgg tggccctgaa gaacctctga
      781 tcattcacct ctccctttta ctgcctgctg ccgagatccc accacacttc ttcatccaag
      841 tcgcagagag aggtcctcga cagagtacgc aggtggtgcg catgagtcca tggtgcagac
      901 atgagcgact gccgcttccc ctgcaaagcg atgcgaagcg atggaagggg cgagtctaaa
      961 caggagttcg tattgcttcc cctgctccag ggaaacggga atcgtgaacg gcaggtaatg
     1021 gcggtcaagg aacgcattca tcccccggaa ggcgggatct gtgccctttc ctgtcactac
     1081 cacgcgggtg gcagggccgg cagagagcgt gaaccccgcc atgaacaagc cgcatgatgc
     1141 aggactcctc tcaaccaggg gggagtaaag acgggagagt gcatcggcac gccggccaaa
     1201 ccgctcttct tgggtaatga gcccaatgcg cacgaggttg cagaacgaga tggagttcgg
     1261 ggaagggatc gccccgtcgg tgaactcctt cctccttgca aagaggtcga tcgcgctctc
     1321 gggtgaagtg aagtatccgc ccgatctttt gtcccagaaa tgggcatcgt ggtagtcctc
     1381 gagcacaagc gcagattcca ggtgccctgg atccctggtc gcgacaaaca gttcgaggag
     1441 gccccagacc attgccgcat agtcggccga gagaccagga atcccggcag agccatccct
     1501 gaaccggtgg tagaggcctc cgtcatccat acgcatccgc gagaggacaa aggttgcagc
     1561 cttttctgct gcttctatat atctgctcct gccagatgcc ctcccggcca tggctagcgc
     1621 ggcaatcgca agcccgttcc agtcggcaag gaccttctca tcgcaaaaag gccttgttcg
     1681 catctcacgg gcccggagca gggtggccct tgccctctca atccgctcgc tgacctctcc
     1741 gggcgaaaga gagagggccc gtgccgcctc tttttcggaa tgggtgcggt ggaggacgtt
     1801 ctccctccca tagcggccgg tgtgcgggtc aagataattt cctgtccggc tgacagggaa
     1861 gaccaggaaa gccacccgcg catcttccgg gtccagtaag gaggcgatct cgtcatgcgt
     1921 ccagaggtaa tacttccctt ccacgccctc gctgtcggca tcctctgccg agaagaaccc
     1981 cccgtccggg gcggagagcc gggagcaggc atagtccagg cagccggatg caacctccca
     2041 aaactcctgg tttcccgtcg cgagccatgc ctcagtgaag gccatcgcgg ccagagcctg
     2101 gtcatagagc atcttctcga agtgcgggac cagccaccgt gcatcagtcg agtaccggtg
     2161 aaatcccccg ccgaggtggt cgtatatgcc gccccttgcc atggagagga gggtcttttc
     2221 ggccatgcca agggctttgc cgctgccggt ccacttccag tagcgtagca ggaagaggtg
     2281 gaggtgcggc atcgggaact tgggcgcagg accgaatccc ccgttcaggc tgtcgtactg
     2341 gaggagaagg tcctggagca tccggtcagc tgtgtccttt ctcacccgcc cgccgggtct
     2401 cgccttgcga tcggcagcga tggcgcgtgc gaggagccct gcggactcca gcgcctcttc
     2461 ctgcttctcc tcccagaact ctgcgagcct tgggaggacc tccagtagtc ccggcatccc
     2521 catcctgttc tccctcggga gatatgtcgc cgcaaaaaac gggtggagat caggggtgag
     2581 gacgacattg aggggccagc cgcccgagcc cgtgagggcg atcgcggcat ccatgtagag
     2641 ctggtcgaga tccggccgct cctcgcgatc caccttgatg cagatgaaat gtgcattgag
     2701 gagggcggca acctcgcggt cagagaaaga ctccctctcc atcacgtggc accagtggca
     2761 ggtggagtat ccgatggaga ggaagatcgg ctttccttca gcccgcgccc tggaaaacgc
     2821 ctcctctccc cacgggtacc agtcaacggg gttatgcgcg tgctgctgca ggtagggtga
     2881 cgcctcgtgg acgagccggt tcgccggatt cgaagaatcc ctctctccag cgtccggatc
     2941 agtcaccggc accgctccct ggcctcaggg actctctctt tcgctcttca tccagtggct
     3001 ctctccttgt tgcccggaaa gcaggaaaga agcagacagc ggcgtagagg aacacaagac
     3061 ccacaccgga ggatatggaa aaagggacac ttgaatattc gcaccggggc aggaaaggat
     3121 aactctccca gtaaccctga gccccgcgat cgttacacat aactctccct atgcacgaga
     3181 atatgagtga tggaaaaggg atcgcggtcc gggggtgccg gggcggagag ccgcctctcc
     3241 cctgccatgg cccagtacca ggagatcaag tcgaaatatc ccgatgctat ccttcttttt
     3301 catatcggag atttttacga gaccttcaac gaggatgccg agaccgtgtc caggcagctc
     3361 gacatagtcc tcacctcccg ttccagggac cgcagcggga acaggattcc tcttgcaggg
     3421 gtgccctgcc atgcggttga agggtatatt gcgaggctga tcgcaaaggg ctacaaggtc
     3481 gcggtctgcg accaggttga ggacgcacgg gctgcgaagg gaattgtcaa gagggaggtg
     3541 gtccgcgtcg tgacccccgg catggtgatg gacgagacac tcattccctc tccgggtgcc
     3601 cactacctcc tcgccatctc ccccaacgcg aaggagggag aggcgggact tgcattcctc
     3661 gacatcacca ccggggagtt ctcggtcgtc acggttcccc tcgaacacgg gttttccgcg
     3721 ctccaggggg aggttgcgag gaattccccc gccgagtgcc tgctgccagc cgacgctccc
     3781 cctgaaatcg cagagttcct ctcgagggga ggccttgcgg tgagccgcgg agcggcggat
     3841 atcttctctc cggagaaggg tcgggacgcc ctgtgcaggc atttcggtgt cgcgagcatc
     3901 gagggattcg gccttgcacg ggactctccc gcggagcagg cagcgggtgc ggcactcctc
     3961 tatgcgaagg agactcagcg gtcggaactc ccccagatca acgccctctc ggtgcgccat
     4021 tctggcgagt cctgcctcct ggatgcgata accctgagga accttgagat cgtgcagggg
     4081 atcagggaca gagggaagga ggggaccctc atttctgtcc tcgatcgcac cgccaccccg
     4141 atgggaagcc ggctcctccg ccagtggctc tgcgagccgc tggtgaacgt cggcgccatc
     4201 aatacccgcc tggacgccgt cggacacctg gtcaggaatg ccgcggccag gatggaggct
     4261 tctgttacgc tccgccgctg cgccgatatt ggacggattg cagggaggat cgcctttgga
     4321 aacgcgaccc cgagggactt aaaaagcctc gagcggtccc ttgccaccat cccgcaatta
     4381 aaagcgatcc tgcgctacgg gcagggggcg gagatccctg catttatcca ggatgcctgc
     4441 gggatgctct ccgatctctc ctgggtgggg gagacgatcg gtgccgcgat tgtggacgat
     4501 ccccctgcgg tggtgcgaaa cggcggcata atccgcgacg gcttctcgcc ggaactggat
     4561 tcgctccggg ggatctcgac atccggaaaa gagtggatcc tggatctcca gcagcgagag
     4621 cgggagagga cgggaatcaa gtcgctcaag attgcctaca actcggtctt cggctactat
     4681 atcgaagtaa cccgccctaa tctctccctc gtcccgcccg agtaccagcg caagcagacc
     4741 acctccaatg gcgagcggtt cactatccat gagttgaagg agaaggaggc ggtcatctca
     4801 tccgcggacg agcgccagtt gtcactcgag caggaggtct attcgggtct cctggaacgg
     4861 ctgaacggag cggtccccat gatgcaggag atcgcccggg gaattgcgct ccttgacgtg
     4921 gcggtttcgc ttgcggaggt ggcagagtcg ggccattact gcaggccggt ggtggacgag
     4981 tccacgcacc tcctggtgcg ggaaggccgc cacccagtgg tcgaggtggg gatggccggg
     5041 aggttcgtgc caaacgatct ggaactctca agcgaaggag accagatcct catcatcacg
     5101 ggggcaaaca tggcaggcaa gtcgacctac atgcgggcgg cagccctcgt tgcagtcatg
     5161 gcgcaggccg gaagtttcgt tcctgccgca tacgctcgta tcggcgtggt ggatcgggtc
     5221 ttcacgcggg tgggggcatt cgacgacctc gcgagcgggc agagcacatt cctcgtagag
     5281 atgctcgagc ttgcaaatat cctcaacaac gtgaccgggc gaagcctggt aatcctcgat
     5341 gagatcggga gggggacgag cactctcgac ggtctctgca tcgcaagagc ggtcttagag
     5401 ttcctgcacg gcaaaaagtc ccaggggcca aggaccctct ttgccacgca tttccatgaa
     5461 ctcgtcactg tcgaggggga gttctcccgc gtgaagaact tccacttcgc ggcaaaagac
     5521 accggcaggg aggtcgtctt cctccggaag atcatccccg gcgcgaccga caagagctac
     5581 ggtatccacg tcgcggcgct tgcgggggtt ccacgcaggg tgactgaccg ggcgcgggtg
     5641 ctcctcgaag agatggccgg tgccgaaggc cgttcatcag gtccgagagt gaagcggtat
     5701 acccagatgc ttctcccaga aatcccggcg gtggacgcgc ctgcccaccc tgtcctcgag
     5761 acactgaaga acatgaacct cgatgatatg actccgctct cggccctctc ccttctctat
     5821 gacctccaga agagggcgcg ggaagggggg cggtgaagga tggcgatcgg gagagaacca
     5881 ggagagatcc aggtactcga ccagcagacc gtgtcccgga tcgcggccgg agaagtggtc
     5941 gagcgacctg cctcggtgat aaaggagctc gtggagaacg cgcttgatgc gggagcaaca
     6001 cgcgtcgagg tggagatcac ctcgcgcaag gggatcatca ccgggatcag ggtaactgac
     6061 aatggttcgg gaatgacgcc aaaagacgca gatctcgcct gtgtccggca tgccacgagc
     6121 aagatccgga ctctttccga tctcctgctc gtccagtcgc tcgggttccg cggcgaggcg
     6181 ctcgcaagca tcgctgcagt ctcgaggctt tccctcatca caaggaaggc gggcaccgcg
     6241 ctctcaggtg tccggattgt aaacgaaggg ggagaggtga ttgagcgggg tgaatgcgga
     6301 tgccccgagg ggacgactgt cgaggtggag gagatcttct tcaacacccc tgcacggaga
     6361 aaattcctgc gctcgctccc ggcagagatg gcctatatca cggggatttt ggagcggatc
     6421 atcctctccc gcccggatgt ctcgtttcgg gtcctccata accggaaggt gctgatgtcc
     6481 gggcccggtg gatctgtcca cgacgcggcg cttcaccttt ttggcaccgg ggtggcacgc
     6541 gccctgatcc cgctggactt cgaggggtcg ttcatcaggg tgggaggggt gatctcccat
     6601 ccctcgctct caaggcagaa cccataccag gtctttctca gcatcaacag gcgaccggtc
     6661 cagtcaaggg cgctctctct cgcggtgaag gcgggatacg gcacccttct ccccggcgac
     6721 aggttcccgg tggccatcct tgacatcacg atcgacccgc accttgtcga cgtgaacgtc
     6781 catcccacca agagagaggt ccgcttgagc agggagaggg aagtgaaggg tgagatatcc
     6841 cgggccgtgg aaacggcgct taaagacatg gatctcacgt tcgaggcccg cgaatcggga
     6901 aaggcctcga cccaggcaac gatctccccc gccagtggtt cacccgccta tcacatcgat
     6961 gaaccttccc tggccacggt tgcagaaccg gggaggtcca tccgtgccga taccgatcga
     7021 cgcctccggc agaccgagct tggactctcc gcagggacag ccgggcaatc acctgtcccg
     7081 gatctcgaag tcctcgggac tcttgacaac acctacatcc tcgcctgccc gaggggtggc
     7141 gaagacctca tcctgatcga tcagcatgcg gcacacgagc gtatcctgta cgaacaggtc
     7201 ctggatacgg ggcaaagaga gcccgaatgc caggagctga tcgtccctgt tccattgcac
     7261 ctgtccccgc gcgaggctgc gatcctcccg gcgcttatcc cactccttga agagattggg
     7321 ttctccatag agaaattcgg gagaggaacg tatgctgtcc gtgccattcc catagttctg
     7381 ggaaagcaga tcgggcagga aggggtccgg gaggttgttg atgccctcct ctccggggac
     7441 gtgcgccagg atttatccga gaaggagcgc atccttaagg taatcgcatg caggggggcc
     7501 atcaaggggg ggactcccct gaccatggag cagtgcaggt cgctgatagc gcaattgcgc
     7561 cggactgagc atcccttcag ctgcccccat ggcaggccga cgatggtcac cttcaaaaga
     7621 agagaactcg acggcctgtt tttcagaacc tgaatgaaag ttcgggtttt tttatcggtt
     7681 gagaggaaag catataggtg aaagggtccg atactagtgc gaacatgttc cgtt
//
LOCUS       DAOQYR010000098         7385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_10436_length_7385_cov_9.545839, whole genome shotgun sequence.
ACCESSION   DAOQYR010000098 DAOQYR010000000
VERSION     DAOQYR010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 7385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7385
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10436_length_7385_cov_9.545839"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..1888
                     /locus_tag="PKM87_09910"
     CDS             185..1888
                     /locus_tag="PKM87_09910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ30422.1"
                     /translation="MSHDIRWWLKVACILALIAGALLTVWNVQNEDHIQRNNLLIKAR
                     IAANPITSDQIEALSGSEADLGSPVYTHLKQTMEGVVAADTQVRFAYLMGMNDDGGIF
                     FFVDSESPDSEDYSPPGQIYWDATVPTRIAFASADESTAGPATDRWGTWITAFIPVKD
                     PDTGELMGIFGLDVDAHNWVMILLGAAIPPILGTGLVLILLLSFIVVIGRNQKEHELL
                     TSSQRAIQESEERYRLLFTRSPVGIVQIDRNGKIVAANQKCAEILGLIPDSLIGFDVR
                     TQLQNPEFRAAFTEIPRREPAHYEGEYRSPGSGRNLFVRLLAHPLFSEDRGYSGAIAI
                     IEDITDKKQAEKALLMANRKLNLLNSITRHDILNQLLALKGYLELAGDTELGSEQQRR
                     FLESAKKASDNIERQISFTKDYQDMGVKSATWQEIGLALTRARGALPLEHLLIKAEGT
                     EYEIFADPLFEKVFYNLYENSLKHGGEGMTTIRISTRETGEGLVILYEDDGVGIPENA
                     KKFLFAQEMTPQKGLGMFLTGQILAITGITIVETGVPGRGARFEMLVPKGGFRKAKSG
                     D"
     gene            complement(1922..2428)
                     /gene="msrA"
                     /locus_tag="PKM87_09915"
     CDS             complement(1922..2428)
                     /gene="msrA"
                     /locus_tag="PKM87_09915"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNQ30423.1"
                     /translation="MEEYHGPEQATFAAGCFWGVEAAFRQVKGVVATEAGYTGGTVSN
                     PTYQQVCSGKTGHAEAVRVFFDPKVVTYDQLLDIFWGIHDPTQVNRQGPDIGTSYRSA
                     IFYHSPAQKAAAEASRDRLQRSGRFGRVPVATEIAPAAEFWKAEEYHQQYFEKCGCGY
                     SATPKYWE"
     gene            2828..3277
                     /locus_tag="PKM87_09920"
     CDS             2828..3277
                     /locus_tag="PKM87_09920"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNQ30424.1"
                     /translation="MAMSMEESGGHWFSQMPRTGRIYQLIDEAGKAGFRSGRVDAIVA
                     LGECRDPRAVDVLVTCCQDRDPSIRMSAVTALGRMRSGRAVPALVRCIQDRDEVQEIR
                     REAASALASTRCESAVEGLRGCLEDEKVDPAVRSLVEEALAQIRTKG"
     gene            complement(3393..4217)
                     /locus_tag="PKM87_09925"
     CDS             complement(3393..4217)
                     /locus_tag="PKM87_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNQ30425.1"
                     /translation="MVAPEKPGIEFPSVVDRAYQEYKRHLKVSDIMSTEVVVTTPQAS
                     MAETARIMGERRIGSLIVVQYGTPIAIVTERDLLSKVLAGEMDLEKTRVADVMSFPLI
                     KICPDLEIREAARTMIRKKGRLAVFQCGMICGVITASDLIREMPDAPETSLVVDDFMT
                     REVVCVKDSQKIEDVARIMGRKRIGSVIVTESGTPRGIFTERDLLSTLIAKGVSLGVP
                     AGEVCSPMLVTAPAGISIHEAAKIMAAKHIRRLPLMKKKKLAGIITARDLVEAYAK"
     gene            4469..4633
                     /locus_tag="PKM87_09930"
     CDS             4469..4633
                     /locus_tag="PKM87_09930"
                     /inference="COORDINATES: protein motif:HMM:NF018096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HNQ30426.1"
                     /translation="MPSFKCFGKNCTFETSAPTEAELMKKIAEHAKAAHNMDPIPPDM
                     LARVKAAIKK"
     gene            4761..5825
                     /locus_tag="PKM87_09935"
     CDS             4761..5825
                     /locus_tag="PKM87_09935"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ30427.1"
                     /translation="MLFLSTAERRQWERYAEEALRESPEPGYWDSAIRKNLIPAFHFY
                     IATFLAAHGEGERGIGWLESGTLAEEEGLFGCGFLLGFLRRHGGRLIVPVAPFQDPRP
                     FIHFAGVPAMKTARQQFVRQCTHSLPVFDRPLRFMDIGCGDGALTAAVLNHLVEAGKA
                     PGISEVLLIEPSPAMADLAEETVRSALPGVDVMVRNSRIQDCSSTIGNSFDIAMSSLA
                     YHHMPLEQKRVNLSRLKPWIDHLVLFEMDANNDTPDLYTPDLALSVYQSYSRIIDFIF
                     THDAPVDLAIDCVDSFMMAEIVSLLTEPRGKRLDYHMLRGQWHALFSEVLGPEFSCRC
                     DSSAYADEYMTLFTLHYGRD"
     gene            complement(5923..6321)
                     /locus_tag="PKM87_09940"
     CDS             complement(5923..6321)
                     /locus_tag="PKM87_09940"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNQ30428.1"
                     /translation="MSEEQICRQCGHCCERWGWGQSGSPADLRPWIEHGRKDILQHVT
                     VYLDNGQKTSGTRITADDLPRITKVRYWQDPSGQPLRHCPFLGRSKDGKAFCGIHDLR
                     PEVCRDYAPWNCGDGEYQNVRCLACRERTP"
     gene            complement(6389..>7385)
                     /locus_tag="PKM87_09945"
     CDS             complement(6389..>7385)
                     /locus_tag="PKM87_09945"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNQ30429.1"
                     /translation="MMLRFAACFGMFSIMRDMHISPNTLPMKMYELSTYSFRHEQKGE
                     VIGLKRLRAFTMPDMHSLCTDMPEALQCFEEQLMMGWETGKDFGTELVGVIRCTRDFY
                     EEHHEWVARMVKVSGCPMLIEVLSERVHYWVAKVDLAAIDGQGRPIENPTVQIDVESS
                     HRFDIKYHLDGREVHPPILHCSPTGSVERVICAILESTARQKVPRLPTWLSPSQVRVI
                     PVAERHLSFAEKIAADLNNAKIRADLDDRDESVGKKVREAGMDWVPYVVVVGDAEVES
                     GRLTVTIRSKSEPNKPFKESFSFDDLVTLVGADCGAMPFRPLYTPMKLSKKARYI"
BASE COUNT         1543 a         2073 c         2155 g         1614 t
ORIGIN      
        1 ttcattgcct gcagggtagc atctgtggag aacgaaataa aggtagatgt cggggaatgc
       61 cgggaatctg cccgcaggct cggaattgca acttctgcct aattttccgg ttcgtatctg
      121 aggggcaaac cggaaaggca ccagccttaa gaggtgccct gtcatatcca ctctgttata
      181 cccaatgtcg cacgatatcc gctggtggtt gaaagttgca tgcatcctgg ccctgatcgc
      241 cggggctctc ctgacggtgt ggaatgtgca gaacgaggac catatccagc ggaacaacct
      301 ccttataaaa gcgaggattg cagcgaatcc catcacttcc gaccagattg aagcactttc
      361 cgggtccgag gcagaccttg gctccccggt atacacgcac ctgaagcaaa cgatggaggg
      421 ggtggtagca gctgatacgc aggtgaggtt tgcttacctc atggggatga atgatgatgg
      481 tgggatcttc ttttttgttg attccgagtc tcccgattct gaagactatt cccccccggg
      541 acagatctac tgggacgcta cggttcccac caggatcgca ttcgcatcag ctgatgagtc
      601 cactgccggt cctgctactg accgctgggg aacatggatc actgcattta tcccggtaaa
      661 ggaccccgac acaggagagc tcatgggaat cttcgggctt gatgtcgatg cgcataactg
      721 ggttatgatc cttttaggtg cggcaatccc tcccattctc gggaccgggt tggtcctaat
      781 cctgctcctc tctttcattg ttgtgattgg acggaaccag aaagaacatg aattgctcac
      841 ctcgtctcag cgggccatac aggagagcga agagcgatac cggctcctct tcacccgttc
      901 acctgtcggg attgtccaga ttgacaggaa cggaaagatt gtcgcggcca accagaagtg
      961 tgcggagata ttggggttga tccccgactc gcttatcggg tttgatgtcc gtacccagtt
     1021 gcaaaatcct gagtttagag ccgcgttcac ggagatccca aggcgtgaac ccgcgcatta
     1081 cgagggggag taccgatcgc ctggttcagg aagaaatttg tttgtgcgac tccttgcaca
     1141 ccccctcttc tccgaggaca gagggtattc cggagcaatt gccattatcg aagatatcac
     1201 cgataaaaag caggctgaaa aagccctcct gatggcaaac cggaaactca acctcctgaa
     1261 cagcatcacc cgccatgata tcctcaacca gctgctcgcg ctgaagggat atctcgaact
     1321 tgccggcgat acggaattgg gcagtgaaca acagcgcagg ttccttgaaa gtgccaaaaa
     1381 ggcgtcagac aacatcgagc gccagatcag tttcacgaag gactaccagg acatgggagt
     1441 gaagagtgcc acatggcagg agatcggttt ggcgcttacc cgggcgcgag gcgctcttcc
     1501 acttgaacac ctcttgatca aggcagaagg gactgagtac gagatatttg cagatcccct
     1561 cttcgagaag gtcttttaca acctctatga aaactccctc aagcacgggg gagaggggat
     1621 gactacgatc aggatctcga cccgcgagac aggtgagggg ctggtgatcc tctacgagga
     1681 cgacggtgtg ggaatcccgg agaatgcaaa aaagttcctc ttcgcgcagg aaatgacgcc
     1741 gcagaaaggc ctcgggatgt ttcttaccgg gcagatcctc gcgattaccg gaatcaccat
     1801 cgtggagacg ggtgttccgg gcagaggggc ccggttcgag atgctggtgc cgaaaggggg
     1861 tttccggaaa gcaaaatccg gcgattgacc ggggcaaaac gcagccgccc gcaatcgcgc
     1921 gttattccca gtatttcggc gtggcagagt acccacagcc gcacttctcg aagtactgct
     1981 ggtgatactc ctcggccttc cagaactcgg ctgcgggtgc aatctcggtt gcaaccggca
     2041 cccttccaaa cctccccgat cgctgcagcc tgtcccgcga tgcctctgct gctgccttct
     2101 gcgcaggcga gtgataaaag atggcggacc ggtaactggt cccgatgtca ggcccctggc
     2161 ggtttacctg cgtgggatcg tgtatccccc agaagatatc gaggagttga tcgtacgtga
     2221 cgacttttgg gtcaaagaaa accctcactg cctctgcgtg gcctgttttt ccggaacaga
     2281 cctgctggta tgtagggttg gagacagtcc ccccggtata acctgcctcc gttgcaacaa
     2341 cccccttgac ctgcctgaac gcggcttcga ctccccagaa gcagccggcg gcgaacgtgg
     2401 cctgttcagg gccatggtac tcttccatgc agtctgattg tgtgggacag ggagaaggag
     2461 gttttggtcc gggaaggctt ggccaatccg ggcgcaaaac ccacatgttc caaaactttt
     2521 ggccggcgag atcgcgctcc tgctaccagt atctcctgcc tttcaggaac ccccggtacc
     2581 cccctctcgt ggaggactgc aaggtcctcc gggagaagga tacctatata tcgtggtgag
     2641 gcaataaaag ggaataatgt accaatctct ccatcgcaca atcaggctat cgggaacggt
     2701 acaggattct gctgctggaa agcggcaacc acttttcacg actaagtgag aactggtttc
     2761 tgctgggtgt tctcccggcc ggggccagga ttggtgtttt tatgtatact ctcgttgtgg
     2821 ggatatcatg gcaatgagca tggaagaatc aggggggcac tggttcagcc agatgccaag
     2881 gaccggaagg atttaccagc tgatagacga agcaggaaaa gcgggattcc ggagtggaag
     2941 ggttgatgcg atcgtggcgc ttggggagtg cagggacccc cgggcagtcg acgtccttgt
     3001 cacctgctgc caggacaggg atccatccat acggatgagt gccgtgacgg cgctcggcag
     3061 gatgaggagt ggtcgggcgg tcccggccct tgtcagatgc atacaggata gggacgaagt
     3121 gcaggagatc aggagggaag cggccagtgc gctcgcttcc acccggtgcg agagtgcagt
     3181 cgaagggctg agagggtgcc tggaagatga aaaggtggac ccggcagtca ggtccctggt
     3241 agaagaggct ctcgcacaga tccggacaaa aggttgaggc ggggtgtgga acacaccgct
     3301 gtgtgtggga ggaggggttc cctctctctg atcttaatac gcacacctgc gtgaccaggt
     3361 cccgctaccc ctggtcccca ggatatcccc gtttactttg cgtaggcctc cacgaggtct
     3421 ctcgcggtga tgatccctgc aagtttcttc ttcttcatca gcgggagcct gcggatatgc
     3481 tttgcggcca tgatcttcgc cgcctcgtgg atactgatcc ctgcaggcgc agtgacgagc
     3541 atcggcgagc agacttctcc ggccgggaca cccagcgaga cgcctttcgc gataagcgtc
     3601 gacaggaggt cgcgctcggt gaatatcccc cgcggtgtgc cgctctcggt cacgatcacg
     3661 cttcctatac gtttccgacc catgatcctg gccacgtcct cgatcttctg actgtctttc
     3721 acgcagacaa cctcccttgt catgaagtcg tcgacaacga gcgaggtctc cggggcatcc
     3781 ggcatctcgc ggatcaggtc cgatgcagtg atcactccgc agatcattcc gcactggaag
     3841 accgcgaggc gccctttctt tcttatcatc gtccgggcag cttcgcggat ctcgaggtct
     3901 gggcatatct tgatcagcgg gaatgacatc acgtctgcaa cccttgtctt ctccaggtcc
     3961 atttctcccg caagcacctt cgagaggagg tcgcgctcgg tgactattgc gatcggcgta
     4021 ccgtattgca cgacgatgag actcccgatc cgcctctccc ccatgatccg tgcggtctct
     4081 gccatagagg cctgcggggt ggtgacaacc acttcggtgc tcatgatgtc ggataccttc
     4141 aggtgcctct tgtattcctg ataggcacgg tcgacgacgc tcgggaattc gatcccgggt
     4201 ttctctggtg caaccataca gatccccaat tggtgtgaga gagcttctgg cggtaaaaag
     4261 gttatggagg aatcgatatg atcgcggtgc ggaggattct ttggcatctc actgttatag
     4321 gtcactggat gaatttgtgt gatgtttttt tgcagaatat cgcgtgattc ccccggtctt
     4381 ttctcccccc tcgcagccgg ttcaatggcg gttggtacga taacaagtga taaataggta
     4441 taatgggatg aatcaacggt ggtgaaaaat gccgtctttc aaatgtttcg gaaaaaactg
     4501 tacgtttgag acctccgctc ccactgaagc ggaactcatg aagaagatcg cagagcatgc
     4561 aaaggccgcc cacaacatgg acccgatccc gccggacatg ctggcaaggg tgaaagccgc
     4621 gataaaaaag tgacacgctc cgaaattatc gcagaatcct ggccgcgtca acacacccct
     4681 cgccgcgggt gattctcttc gcccacggtt caatactctt tttaccaacc gaatcatgat
     4741 actccgggag aagagagacc atgctatttc tatcgacagc agagagaagg caatgggaaa
     4801 gatatgcgga agaagcgctc cgggagagtc cggagccggg atactgggat tcagcgattc
     4861 ggaagaatct catacctgcg tttcacttct acatcgccac gttccttgcc gcacacgggg
     4921 agggtgaacg gggaatcggg tggcttgaga gcggcaccct cgccgaggag gaagggctct
     4981 ttgggtgcgg tttcctcctc gggtttttga ggcggcacgg ggggaggctc atcgtacctg
     5041 ttgcaccgtt ccaggatccg cggccattca tccatttcgc aggggttcct gcgatgaaaa
     5101 cagcgaggca gcagttcgtc agacagtgca cacattctct cccggtcttt gaccggcctc
     5161 tccggttcat ggatatcggg tgcggcgatg gggcgctcac cgctgcggtc ctcaaccacc
     5221 tcgtggaggc gggaaaggcc cctggcatca gcgaggtgct cctgatagag ccgtcgccgg
     5281 cgatggcaga tcttgcagaa gagacggtcc gctctgccct tcctggtgtg gacgtcatgg
     5341 tccggaattc gcggatccag gactgttctt cgacaatagg caattccttt gacatcgcca
     5401 tgagttcgct tgcctaccat catatgccgc tcgagcaaaa aagggtgaac ctctcccggc
     5461 tgaaaccatg gatcgatcac ctggtactct tcgagatgga tgcaaacaac gacaccccag
     5521 acctctacac ccccgacctt gccctctccg tctaccagtc ctacagccgt atcattgatt
     5581 tcatcttcac ccatgacgcc ccggtggatc tcgccataga ctgcgtggac tctttcatga
     5641 tggcagagat cgtttcactc ctgacggagc cgagaggaaa gcgcctggat taccacatgc
     5701 tccggggcca gtggcatgcg cttttcagcg aagtgctcgg gcccgagttc tcgtgccggt
     5761 gcgactcatc cgcctatgcc gacgagtaca tgacgctttt caccctccat tacgggaggg
     5821 actgataaga aagtggagag gatttggggg gtggcctgcc gcacggggtt acgcacctga
     5881 tgtgcggcgt ccgatgggga ttgcggcgaa tcttcctgcg gttcaggggg tcctctccct
     5941 gcacgcaagg caccttacat tctggtactc gccgtcgccg cagttccatg gtgcataatc
     6001 cctgcagact tccggccgca ggtcatggat gccgcagaag gctttcccgt ctttcgatct
     6061 gcccaggaac gggcagtgtc gcaggggctg gccggaaggg tcctgccagt aacgcacctt
     6121 tgtgatgcgg gggagatcgt ccgccgtaat cctggtgccg ctggtctttt gcccgttgtc
     6181 aagatacacc gtcacgtgct gaaggatgtc tttgcggccg tgttcaatcc agggacggag
     6241 atctgcggga gacccgctct gcccccaccc ccaccgctcg cagcaatgcc cgcactgcct
     6301 gcagatctgt tcctcgctca cgctggagaa gtgtgttcac cgggtaaaga aaaaaaggtg
     6361 agggtattgg tctgaccgag ggctcttttc agatgtacct ggccttcttc gagagcttca
     6421 tcggggtata cagcgggcgg aacggcattg ccccgcaatc ggccccgacc agggtgacga
     6481 gatcgtcaaa cgagaacgat tccttgaacg gcttgttcgg ctcggacttg gaacggatgg
     6541 tgaccgtaag tctcccgctc tcgacctccg catccccgac caccacgacg taagggaccc
     6601 agtccatccc tgcctcgcgg actttctttc ctacgctctc gtccctgtca tcgagatccg
     6661 cccttatctt tgcgttgttc aggtcggctg cgatcttctc cgcaaaagag aggtgacgct
     6721 cggcgacggg tatcacccgc acctgggagg gtgagagcca ggtgggaagc ctcgggactt
     6781 tctgcctggc agtgctctcg agtatcgcgc agatgacccg ctccacggag ccggtcggcg
     6841 agcagtggag gatggggggg tggacttccc taccgtcaag atggtacttg atatcgaacc
     6901 ggtgcgagct ctccacgtcg atctggaccg tggggttctc gatgggccgg ccctggccgt
     6961 cgattgccgc gaggtcgacc tttgccaccc agtagtggac ccgctcggag aggacctcta
     7021 ttagcatggg gcagccagag acctttacca tccgtgcgac ccattcgtgg tgttcctcgt
     7081 agaaatccct tgtgcaccgg atcacgccga cgagctcggt gccgaagtcc ttccctgtct
     7141 cccatcccat catgagctgc tcctcgaagc attgcagggc ctcgggcatg tcggtgcaga
     7201 gggagtgcat gtcgggcatg gtgaatgccc ggaggcgctt cagcccgatc acctccccct
     7261 tctgctcgtg gcggaacgag taggtggaga gctcgtacat cttcatcggg agggtgtttg
     7321 gggagatgtg catgtcccgc atgatagaaa acatcccaaa gcacgcggcg aaccggagca
     7381 tcatg
//
LOCUS       DAOQYR010000099         7287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_10622_length_7287_cov_9.609790, whole genome shotgun sequence.
ACCESSION   DAOQYR010000099 DAOQYR010000000
VERSION     DAOQYR010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7287
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10622_length_7287_cov_9.609790"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..251)
                     /locus_tag="PKM87_09950"
     tRNA            complement(178..251)
                     /locus_tag="PKM87_09950"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(215..217),aa:Val,seq:cac)
     gene            complement(255..325)
                     /locus_tag="PKM87_09955"
     tRNA            complement(255..325)
                     /locus_tag="PKM87_09955"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(291..293),aa:Gly,seq:tcc)
     gene            complement(453..1331)
                     /gene="htpX"
                     /locus_tag="PKM87_09960"
     CDS             complement(453..1331)
                     /gene="htpX"
                     /locus_tag="PKM87_09960"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HNQ30430.1"
                     /translation="MKVWKRDFGLTLRIWLTLFLLFLVYLVFLAVLSWLGVGLPFLVL
                     LVFGMAFIQYFFSDKLVLWSTRARVIEPEEYPELHRMVQRLADEAGIPKPRVAIMQTP
                     VPNAFATGRSPKHAVVAVTDSIMRLLTPRELEAVLAHELSHVKNRDVLTLTIASFVAM
                     LAALIMNNFLFASLFSRRDDNPWIIAGIVAIVVYFVAQLLIMALSRYREFAADRGSAY
                     LTGRPADLISALEKISGRMDRVPAEQKRVVESANAFFIIPALSGSTLMELFSTHPPLE
                     KRIAALEKVEMEMRGY"
     gene            1515..2294
                     /locus_tag="PKM87_09965"
     CDS             1515..2294
                     /locus_tag="PKM87_09965"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HNQ30431.1"
                     /translation="MELDEITISRAILSSQVEKLLEFMEMDVAVVGGGPSGLTAAALI
                     GEQGFRVGLIEKKLSVGGGMWGGGMMFPRIVVQEEAKRLLDQFDIAHTSYTEGYYVAS
                     SVEAVSKLTASACDAGVEFFNLFSVEDVMIRGDTRLSGLVVNWTPVEMAGLHVDPLTM
                     GCRVAVDATGHDSVLARLVERKGGDVKVRGEGFMWADRAESEIVSHTREVFPGLVVCG
                     MAANAVAGEHRMGPVFGGMLLSGERAAALATSSLRQENSAA"
     gene            2326..2976
                     /locus_tag="PKM87_09970"
     CDS             2326..2976
                     /locus_tag="PKM87_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1917 domain-containing protein"
                     /protein_id="HNQ30432.1"
                     /translation="MSTIDPDTLPDVACGLLFHLLNRELQDQGIFLFSLVEERIDFRD
                     RFLGLFGRFCGDYPELGEALLHKYGSVDAVYELICQGEGVMPGKTTTMHWIVQDAPDV
                     HPDAIEDELAGKWLIFLPPEEVDPAWTIVRDATCRNELGISAKVSTARPNPDSRDNRK
                     VIYVYTADWQDEADVMRVRERLRELGFVDRLGYKRNIETYKGEYSEKGKKVTFYSA"
     gene            complement(2992..3138)
                     /locus_tag="PKM87_09975"
     CDS             complement(2992..3138)
                     /locus_tag="PKM87_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L40e"
                     /protein_id="HNQ30433.1"
                     /translation="MARFPEAEAKLLNVKICMRCNARNAMRATRCRKCGYQNLRPKNK
                     ERKA"
     gene            complement(3199..3765)
                     /gene="rdgB"
                     /locus_tag="PKM87_09980"
     CDS             complement(3199..3765)
                     /gene="rdgB"
                     /locus_tag="PKM87_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNQ30434.1"
                     /translation="MKFAVVTGNPHKAREIAQFFAGVAEVEHIPLECPETRDDDVGVI
                     SAKKAEYAYGILQRPLMTDDTSFCIPALGGFPGPYAAYVQKTIGNRGIIRLMEDETDR
                     SAYFETAVAFADEQGISVFRGRIHGRIVSPRGEEGFGYDPVFEWEGRTLAEISLEEKS
                     RVSHRARALANLRDWLEGRERERSHLPG"
     gene            complement(3762..5354)
                     /locus_tag="PKM87_09985"
     CDS             complement(3762..5354)
                     /locus_tag="PKM87_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional N(6)-L-threonylcarbamoyladenine
                     synthase/serine/threonine protein kinase"
                     /protein_id="HNQ30435.1"
                     /translation="MNRRHTPVPGQVLGIEGTAWNLSAALFEDDLVDLYSKPYQPPQG
                     GIHPREAAQHHASEMRGVIGRVMKDPSRVSGIAFSMGPGLGPCLRTVATAARALALSL
                     RVPLVGVNHCIAHVEIGRFATGCDDPVVLYASGANTQVIGYLNGRYRIFGETLDIGLG
                     NALDKFARSRGLPHPGGPAIEELATRGSYLDLPYTVKGMDLAFSGLVSAAKEQDAPME
                     DICYSLQETAFAMCVEVTERAIAHSGKDEVLLVGGVGVNRRLQEMLSVMCSERGATLH
                     VPPPKYMGDNGAMIAYTGKIMLEAGCAMDPEETRVNPGFRSDQVEVTWREDDQTTKTG
                     PGVSAKLSKARGAEAEVTFIGDAVEKRRVRKPYRQGTLDTRLIAERTRAEARLISMAR
                     RCGVPTPVIRDATPDTLVMEKVEGRLLKETLCEADLMEAGRTVGRLHAAGIVHGDLTT
                     SNMIMRDSRCVLIDFGLSAVSGEVEARGVDIHVLFQTLESTTDTYPELRQAFCEGYRS
                     AFAGANEVLAREQEISQRGRYL"
     gene            complement(5447..5635)
                     /locus_tag="PKM87_09990"
     CDS             complement(5447..5635)
                     /locus_tag="PKM87_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27ae"
                     /protein_id="HNQ30436.1"
                     /translation="MAAKKKAKAASVKRGSYFKVEGGKVSFSRKYCPRCGPGIILADH
                     PDRAACGKCGYTEFKKPA"
     gene            complement(5638..5934)
                     /locus_tag="PKM87_09995"
     CDS             complement(5638..5934)
                     /locus_tag="PKM87_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S24e"
                     /protein_id="HNQ30437.1"
                     /translation="MEFEVTRDTRNELLGRREVDFTLQFDGPTPSRKQILGKISALMN
                     VQEKQVVVDSLKTSFGMTACTGSARVYDSEERRNQIERAYLMNRGMPKPKEEGA"
     gene            complement(6011..6505)
                     /locus_tag="PKM87_10000"
     CDS             complement(6011..6505)
                     /locus_tag="PKM87_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-dependent dephospho-CoA kinase family
                     protein"
                     /protein_id="HNQ30438.1"
                     /translation="MLRLPESQRHHFKSPFGTLVPDIADLADELPGKRLYTVGDVVTR
                     NVLEMGLLPEVAVVDGHTMREPCSRAPEVFPAVFPAKNPPGTITPMLVEALKKAVANP
                     PALVVVEGEEDLAVIPLVFEAPEGAWILYGQPCKGVVVREIDEEARATAKSLLACFIE
                     EAEG"
     gene            complement(6505..6708)
                     /gene="spt4"
                     /locus_tag="PKM87_10005"
     CDS             complement(6505..6708)
                     /gene="spt4"
                     /locus_tag="PKM87_10005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor subunit Spt4"
                     /protein_id="HNQ30439.1"
                     /translation="MAAVRKKLVHVCRECHRVVEGEACAVCGSSNLSTDWTGYLVIID
                     PKRSEVARKMNITLPGRYALKVR"
     gene            complement(6695..>7287)
                     /locus_tag="PKM87_10010"
     CDS             complement(6695..>7287)
                     /locus_tag="PKM87_10010"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HNQ30440.1"
                     /translation="YYKMQLADKVRVPPNRLGEDLTRVILDVLQEHLEGSIDKEIGIF
                     ISITKVLRIGEGEIVPGDGAVYYDVDFEAVVLRLALQEVLEGLVVETTSFGAFVSLGP
                     IDAMLHVSQISDEYINYDEKNARLICQESKRFIGVGEPVRVRVVTLSLNEREPRDSKI
                     GLTMRQSGLGTARWLEEDMLKEKEKEKEKGGERGSR"
BASE COUNT         1434 a         2207 c         2073 g         1573 t
ORIGIN      
        1 atttaatctc actttaatta tattaaatga accggtgtga atttatctta taaatcgtgt
       61 tacttaatgc gagccgcatt accagtcttg gagacattct ttttgaattt tgtaaccatt
      121 cgtttcgata aaaggaatct tccagacggg cataaggtca aatgcgcctg taagtgatgg
      181 gctcgaggag attcgaactc ctgacctccg ccgtgtgaag gcgacgtcat aaccagctag
      241 accacgagcc cgagtgcatc gaccgggaat tgaacccggg ctataggctt ggaaggccta
      301 agtcatgcca ctagaccatc gatgcagtgt gcctctttat tatgaccata attggtatta
      361 tgctttgtgt tcttcgtccg cacaccgatt cggggccgct gatgagagga ctaaaaaaag
      421 aaaatatgcc ggctttggga taccgcgagg ggttagtacc ccctcatctc catctccacc
      481 ttttcaagag ctgcaatacg cttctcgagc ggtgggtgcg tggagaagag ctccatcagg
      541 gtgctgccgg agagggccgg gatgatgaag aacgcattgg cgctctcgac cacccgcttc
      601 tgttccgccg ggacgcggtc catccgcccg ctgatcttct cgagggcgct gatcaggtca
      661 gccggcctcc cggtcaggta ggcgctcccc cggtccgcgg caaactcccg gtagcgggag
      721 agtgccatga taaggagctg ggccacgaag tatacgacga ttgcaacaat ccctgcgatg
      781 atccagggat tgtcgtccct tcgggagaag aggctcgcaa agaggaagtt gttcatgatc
      841 aatgctgcga gcatggcaac gaaactggcg atggtcaggg taaggacatc ccggttcttc
      901 acgtgggaga gttcgtgcgc gaggaccgcc tcgagctcgc gcggggtaag gagccgcatg
      961 atggaatcgg tcaccgccac cacggcatgt ttcgggctgc gcccggtcgc aaacgcattt
     1021 ggcaccgggg tctgcatgat ggcaaccctc ggcttcggga tgcccgcctc atcggccagc
     1081 ctctgcacca tgcggtgcag ctcgggatac tcttcgggct cgatcacccg ggctctcgta
     1141 ctccagagca cgagcttgtc cgagaagaag tactggatga aggccattcc gaataccagg
     1201 agcacaagga acggaagtcc cacgcccagc catgagagga ccgccaggaa gaccaggtac
     1261 acgagaaaca gcaggaacag ggtcagccag atcctcagcg tgagtccgaa gtcgcgcttc
     1321 catactttca tagtgagtac ttttctgtaa ccgacgacat aaacattctc tcgggggcag
     1381 ggaaaaaacg ggaaactccg caaaaaaggg agaaaagata ttatagcagg cgaaaggccc
     1441 cgcatgggat ttgccgccgc acctttatgc gtatatttac agtggagagg gtacaaagta
     1501 tgtgatacga taccatggag cttgacgaga tcacgataag cagggcgatt ctctcctcac
     1561 aggtggagaa gctgctggaa ttcatggaga tggacgttgc agtcgtggga ggaggccctt
     1621 caggcctcac tgccgccgcc ctcatcgggg aacagggctt tcgggtcggc ctgatagaga
     1681 agaaactttc cgtgggcggc gggatgtggg ggggaggcat gatgtttccc cgcatcgtgg
     1741 tccaggaaga ggcaaagcgg ctcctggacc aatttgacat cgcccatacc tcctacaccg
     1801 agggatatta tgtggccagc tcggtggagg cagtctcgaa actgaccgct tctgcatgcg
     1861 atgcgggggt ggagttcttc aacctctttt cggtcgagga cgtgatgatc aggggcgaca
     1921 cccgcctctc ggggctggtc gtgaactgga caccggttga gatggccggg ctccacgtgg
     1981 accctctcac catgggatgc agggtggccg tggatgccac ggggcacgac tcggtccttg
     2041 ccaggcttgt ggagcggaaa gggggtgacg tgaaggtcag gggagaaggg ttcatgtggg
     2101 ccgatcgcgc agagtctgag atcgtttccc acacgagaga ggtcttcccc ggactggtgg
     2161 tgtgcggaat ggcagcaaat gccgttgcag gggagcaccg gatggggccg gtcttcgggg
     2221 gaatgctcct ctcaggcgag cgggccgcgg ccctcgccac ctcgtccctc cgccaggaga
     2281 attccgcagc ataacctttt ttcccgtttc cggcaaccct actgtatgag cacgatcgat
     2341 ccggacaccc tcccggacgt ggcctgcggc cttctctttc acctgctcaa ccgggaactg
     2401 caggaccagg gcatattcct cttctccctg gtagaggaac ggatcgactt tcgtgaccgt
     2461 ttcctgggcc ttttcgggcg cttttgcggc gattacccgg aacttggcga ggcgctccta
     2521 cataaatacg ggagcgtgga tgcggtctac gaacttatct gtcagggaga gggggtaatg
     2581 cccgggaaga ccaccaccat gcactggatc gtccaggacg cccccgatgt ccaccccgat
     2641 gccatcgagg acgagcttgc cgggaaatgg cttatcttcc tcccccctga ggaggtggac
     2701 cctgcctgga caatagtccg ggacgccacc tgccgaaacg agctcgggat atccgcgaaa
     2761 gtgagcaccg cccggccgaa ccctgactca agggacaacc ggaaggttat ctacgtctac
     2821 actgctgact ggcaggacga ggctgacgtg atgcgggtcc gggagagact ccgcgagctc
     2881 gggtttgtgg accgcctggg gtacaagagg aacatagaaa cctacaaggg ggaatacagc
     2941 gaaaagggca aaaaagtgac gttctattca gcctgaagag ccaggcctct attatgcctt
     3001 cctttccttg ttcttgggcc ggaggttctg gtagccgcac ttgcggcacc tggttgcgcg
     3061 catggcgttt ctggcattgc atcgcatgca tatcttcaca ttgagcagtt ttgcttcagc
     3121 ttccggaaat cgtgccattt cagaacacca ctatttagtc tttaaataaa ggggctgcag
     3181 gcacaaaaag attgtgtgtc acccgggaag gtgggacctt tcccgctccc ttccttcaag
     3241 ccaatccctg agattcgcaa gtgcccgcgc ccggtgcgag actcggctct tttcctccag
     3301 ggagatctcg gcgagcgtcc ttccctccca ctcgaagaca gggtcgtacc caaagccctc
     3361 ttcgcccctg ggggatacga tcctgccgtg tatcctgccc cggaagaccg agatcccctg
     3421 ctcgtctgca aacgcaacgg cggtctcaaa atacgcgctc ctgtccgttt catcctccat
     3481 gaggcggatt atcccccggt tcccgatggt tttctggaca tatgcggcgt atgggcccgg
     3541 gaatccgcca agggcaggga tgcagaagga agtatcgtcc gtcatcaggg gtctctgcag
     3601 gatgccatat gcatactccg ctttcttcgc cgagattact cccacatcgt cgtccctggt
     3661 ttcagggcat tcgaggggga tatgctcgac ctctgccaca cccgcaaaga actgcgcgat
     3721 ctcgcgggct ttgtgggggt tcccggtcac caccgcgaac ttcacaggta tcgccctctc
     3781 tgtgagatct cctgttcgcg cgccagcact tcgtttgcac cggcgaatgc ggagcggtat
     3841 ccttcgcaga atgcctgcct cagttcgggg taggtgtcag tcgtgctctc cagagtctgg
     3901 aagaggacat ggatgtcaac accgcgtgcc tcgacctccc cggataccgc ggaaaggccg
     3961 aaatcgatca gcacgcaccg ggagtccctc atgatcatgt tgctggtcgt caggtcgcca
     4021 tggacgatcc cggcagcatg gagtctccct accgtcctgc cggcttccat gagatctgcc
     4081 tcgcaaaggg tctctttcag gagcctcccc tccaccttct ccatcaccag ggtatcgggg
     4141 gttgcgtccc ttatcacggg tgtcggaact ccgcaccgcc gggccatcga tataaggcgc
     4201 gcctcggctc ttgtcctctc ggcgatgagc cgggtgtcca gggttccctg gcggtaaggc
     4261 tttcgcacgc ggcgcttctc gacggcatct ccaataaatg tgacctccgc ctcggccccg
     4321 cgtgccttcg agagtttcgc ggatactccc ggtccagttt ttgtcgtctg gtcatcctcc
     4381 cgccaggtga cctctacctg gtccgaacga aaaccggggt tcacccgtgt ctcttcaggg
     4441 tccatggcac agcccgcctc gagcatgatc ttccccgtat acgcgatcat ggcgccgttg
     4501 tcccccatgt acttcggcgg aggcacgtgg agcgttgcac ccctctcact gcacatgacc
     4561 gagagcatct cctgcagacg cctgttcaca cccactcctc ccaccaggag cacctcgtct
     4621 ttgcccgagt gcgcgattgc cctctccgtc acctccacgc acatcgcaaa cgccgtctcc
     4681 tggaggctgt agcagatatc ctccatgggg gcatcctgct cctttgccgc gctcacaagg
     4741 cccgagaacg cgaggtccat ccccttgact gtatagggga ggtcaagata ggagcctctt
     4801 gttgccagtt cctcgattgc gggaccgccg gggtggggaa gccccctgct ccgcgcgaac
     4861 ttgtcgagcg cgttgcccag gcctatgtcc agggtctccc cgaagatccg gtaacggccg
     4921 ttcaggtatc ctatcacctg ggtattcgca ccgctcgcat agagtaccac cgggtcgtcg
     4981 cacccggtgg caaacctgcc gatctccacg tgcgcgatgc aatggttcac ccccacaagc
     5041 ggcacccgaa gcgagagtgc gagtgcccgg gccgcagtcg ccacggtgcg caggcacggg
     5101 ccgaggccgg gacccatgga gaatgcgatc cccgagactc tcgacgggtc cttcatcacc
     5161 ctgccgatca cccccctcat ctccgaggcg tggtgctgcg ctgcttcgcg tgggtgaatc
     5221 cctccctgcg ggggctgata gggctttgaa tagaggtcaa cgaggtcgtc ttcaaaaaga
     5281 gcggcgctga ggttccaggc cgtaccctcg atccccagga cctgtccggg gacaggcgtg
     5341 tgtctcctgt tcatggaaat atgccggagg ggttgtatgt cacgtgatgc caagagagag
     5401 aatcagggca cgtggtgtta ccccaatgaa aatgcctccg gcattctcat gcaggcttct
     5461 tgaactcggt gtacccgcac ttgccgcacg ccgcccggtc cgggtggtcg gcaaggataa
     5521 tgccagggcc gcacctgggg cagtacttcc tggagaacga gactttcccc ccctccactt
     5581 tgaagtaact ccccctcttg acagaggctg cctttgcctt cttctttgcc gccatcttca
     5641 ggcaccctct tccttgggct tgggcattcc acggttcatg agatatgcgc gctcaatctg
     5701 gttgcgcctc tcctcgctgt catataccct cgcgctcccc gtgcaggccg tcatcccgaa
     5761 actcgtcttc agcgagtcca ccaccacctg tttctcttgc acgttcatca gtgcggaaat
     5821 cttccccagg atctgcttcc tggaaggtgt ggggccgtcg aactggagtg taaagtcgac
     5881 ttcacgcctc ccgagcagct cgttcctggt atccctggtg acctcaaatt ccatcggtat
     5941 cctcttatca tacgcagtaa aattatttat attctatctc gttgtcggat cgattgggaa
     6001 aaatctccca tcatccttca gcttcttcga taaaacaggc aagaagagat tttgccgtcg
     6061 cccgtgcctc ctcgtctatc tcccttacga cgactccctt gcacggctgt ccatagagaa
     6121 tccatgcccc ttccggtgcc tcgaagacaa ggggaatgac cgccaggtct tcctctccct
     6181 cgaccaccac cagcgcgggc ggattggcaa ctgccttctt caatgcctcg accagcatgg
     6241 gggtgatggt cccgggtggg ttctttgcag gaaacaccgc aggaaagact tcgggggcac
     6301 ggctgcaggg ttccctcatc gtgtggccgt ctacaacggc aacctcgggg aggaggccca
     6361 tctccagcac gttcctggtc accacgtcgc cgacagtata gaggcgcttt cccggaagtt
     6421 catcggcaag atcggcgatg tccggcacga gcgtgccaaa aggtgacttg aaatggtggc
     6481 gctggctctc gggaagacgg agcatcagcg gaccttcagc gcgtaccggc cgggaagggt
     6541 gatattcatc ttcctggcaa cctcggatcg cttggggtcg atgatcacaa gataccctgt
     6601 ccagtccgtg gagaggttgg aactgccgca taccgcacag gcctcgcctt ctaccaccct
     6661 gtggcattca cggcatacat ggaccagttt cttcctaacg gctgccacgc tcgccaccct
     6721 tctccttctc tttctccttc tccttgagca tgtcttcctc gagccagcgc gcggttccga
     6781 ggcccgactg gcgcattgtc aggccgattt tgctgtccct cggctcccgc tcgttcaggg
     6841 agagggtgac cacccttacc ctgacaggtt cgccgacgcc gatgaaccgc ttggattcct
     6901 ggcagataag acgggcgttc ttctcgtcgt agttgatgta ttcgtcggag atctggctca
     6961 cgtggagcat ggcgtcgatg ggcccgaggc tcacgaacgc accaaaactg gtggtctcca
     7021 ccaccaggcc ctcgagcacc tcctggagag ccaggcggag caccaccgcc tcgaagtcca
     7081 cgtcgtaata gaccgcgccg tccccgggaa cgatctctcc ttctccgatc ctcagcacct
     7141 tcgtgatgga gataaaaata ccaatctcct tgtcgatgct cccttccagg tgttcctgca
     7201 gcacgtccag gatcactctc gtgaggtcct caccaagccg gttgggaggt acccgtacct
     7261 tgtccgccag ttgcatcttg taataca
//
LOCUS       DAOQYR010000100         7271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_10644_length_7271_cov_9.052522, whole genome shotgun sequence.
ACCESSION   DAOQYR010000100 DAOQYR010000000
VERSION     DAOQYR010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10644_length_7271_cov_9.052522"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PKM87_10015"
     CDS             complement(<1..211)
                     /locus_tag="PKM87_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30441.1"
                     /translation="MTVLQSLKNFVRSKSIHVAYVDVGSCNGCDIEVLACLAPRYDIE
                     QYGIFVHNNPREADVLLVIGAYTPGW"
     gene            complement(208..597)
                     /locus_tag="PKM87_10020"
     CDS             complement(208..597)
                     /locus_tag="PKM87_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1959 domain-containing protein"
                     /protein_id="HNQ30442.1"
                     /translation="MREPIYEKDLIAMKYAILESRRHDRMVREIAAEFGIPQNRMRRY
                     LMDCCDMLLLENLPARYEQGKRVQEEAPEPERQLGAHLFTRAVPLLGEDRMLQILDRV
                     KELARGGTPIDQAVRVGKEMIREAITG"
     gene            complement(594..836)
                     /locus_tag="PKM87_10025"
     CDS             complement(594..836)
                     /locus_tag="PKM87_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30443.1"
                     /translation="MIREVLVVGTLIYLLIIAMQCFDRPALWLQGLAIGAALALATTV
                     YWCREDLRLRTWEAIALWAAVLLFVAYGLARLGGLI"
     gene            complement(833..1675)
                     /locus_tag="PKM87_10030"
     CDS             complement(833..1675)
                     /locus_tag="PKM87_10030"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNQ30444.1"
                     /translation="MIAELLFAVFFGLLLLGIHRKVIARIQRRPGPPVWQEILHMLKF
                     SFKTTWVPATASTALFCGVVLVAIGIWTAALVVVLSGGSLLVIFAIYMLHKIVEHGFG
                     LSSGSPYGKFGGVRSVISAASEIPLFVSVAAIAIFTQSLMITDIIAYQEINGPLLITV
                     PLAALAMYVVLLSKMPFGPFSIVESKELVSGYKTEHFGVWRAGLEVCNGLKTYVLLLT
                     FILVFIGNLPFWLVILAMVIILITLSFVCALSPMLSPYDSVTVQTLIAGLMVAYVALL
                     GVMS"
     gene            complement(1678..1881)
                     /locus_tag="PKM87_10035"
     CDS             complement(1678..1881)
                     /locus_tag="PKM87_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30445.1"
                     /translation="MNKQYALALMLVSAVSAAGALALNYWGILPFWAYAAVLIAGFPL
                     FLLGLGLYWMAREGEADMPFLGY"
     gene            complement(1892..2545)
                     /locus_tag="PKM87_10040"
     CDS             complement(1892..2545)
                     /locus_tag="PKM87_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30446.1"
                     /translation="MNEIGGAPLFFLEFGDIVIYFTPFTLTLFILALVFTVFVLVSRP
                     ERQIDIAFGGDAYYAKEISLSELRFRRFMSIACGLACMGAMVTGDVFDFTLFVSLVGI
                     CNVGIVAAVKSRHVQNAAYQYGLVALAGTVPMFSAAAMIAATTGTLSMLELAKGMILI
                     PVAAKALLVLGVMGEGMAPFYAAKAEMFRAPGAPYVIMCSLSSLLIFLRVAEVILLL"
     gene            complement(2542..3144)
                     /locus_tag="PKM87_10045"
     CDS             complement(2542..3144)
                     /locus_tag="PKM87_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2105 family protein"
                     /protein_id="HNQ30447.1"
                     /translation="MWNEYSIGLAAAFACALIGFLALVWERDDLHKLLLIDLVETASL
                     AVIALIGTDLSEALILPGLVVGIAELLALSQIYLTKEGYQHHPVTRLNIEVMMRSPAP
                     VIISAILVVYGIILSGFTGGGIAGLGIVFLLMSRSFSGDFHALEMASGISWALWVAAF
                     FVFMFAPQWWYMALMGAAIGILLKVIAKMSLVGAMWGEPE"
     gene            complement(3151..3600)
                     /locus_tag="PKM87_10050"
     CDS             complement(3151..3600)
                     /locus_tag="PKM87_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2106 family protein"
                     /protein_id="HNQ30448.1"
                     /translation="MIIQRISRVLSNFDNILPVFAAACAAMSVFVLLSLPLPYSEDQL
                     YPKQIVPGSTLNPYDRGGPPFTQVSIHSQYPENSPTAGYVTAYLTPLSLFISEHTAYM
                     GTTIVAHPGGILDEILYNTRGLDTVVEPTILFVAFAIAAYIFRKREA"
     gene            complement(3597..3836)
                     /locus_tag="PKM87_10055"
     CDS             complement(3597..3836)
                     /locus_tag="PKM87_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2107 family protein"
                     /protein_id="HNQ30449.1"
                     /translation="MNPEFILGLVLLLGGTIAAAFPVPKTYLNRLINLEISAWGLLLL
                     MLSYNEALALLTFGGVSALSVYIFVRVIQKKEAPA"
     gene            complement(3833..4054)
                     /locus_tag="PKM87_10060"
     CDS             complement(3833..4054)
                     /locus_tag="PKM87_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2108 domain-containing protein"
                     /protein_id="HNQ30450.1"
                     /translation="MALIAFGALAIIGALLTIVERDPFRKLISLGILVGGSVPFIVDR
                     GLLDVAIAVALIAPLSTIFILMVCGRGDQ"
     gene            complement(4056..4313)
                     /locus_tag="PKM87_10065"
     CDS             complement(4056..4313)
                     /locus_tag="PKM87_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2109 domain-containing protein"
                     /protein_id="HNQ30451.1"
                     /translation="MTPQEAALAVCAVIAVYAAIRVALEPDTLRKLPFLNVLSFAVAG
                     SIALLIPHPLSIVAAAAYFVGSTLESNAIASTYAGGLDRRE"
     gene            complement(4310..4807)
                     /locus_tag="PKM87_10070"
     CDS             complement(4310..4807)
                     /locus_tag="PKM87_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30452.1"
                     /translation="MDSILPAFARILVAAAVCWLNFILIDLFFRLPAGGGVVGATAIG
                     THIERGGGAIHGGYMMGNIVSSPDASAGTLLASCGVYIAGIPGGLLAAFLVFIGNRIC
                     HDRGYAGTTGAITATFIVWGFSQIGFSPAEFIAGMVIAILTIQGISHRHSSRLLAYLW
                     KVREG"
     gene            complement(5026..6498)
                     /locus_tag="PKM87_10075"
     CDS             complement(5026..6498)
                     /locus_tag="PKM87_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNQ30453.1"
                     /translation="MKRLETLAMIAHDMGVIFEFLGFVTLIPLLVLVIFGEWDMVVPM
                     ASVPLTFVILGLLISRIPRRDYIPQLSVALAAVALIWLAIAVVGSLPFVLGLHMTWTD
                     SIFEAMSGWTDSGITMIRSLDTTPRTLLFYRSFMQWIGGIGVIAFGIAMLSRSSLTQH
                     QLYRSEGRSEALMPNVVSTGRRMWVIYLFLTILFTGLVMLSGIPLWDALNLAMVGIST
                     GGFSIHDAGISFYDNALLEAIMIPVMIAGALPFKLYFFMYRGRIRPFLQDRVVWLILS
                     LILVGSLVITLDLHVFNSFPLETAVRQGVFCTASAVTSTGLQNSDLHWVAAPLIVITL
                     LMMIGGASGSTAGGIKANRVILGYEGLVWWFRRFFARGRAIVPFRHEGKAIPRQISEV
                     ELSKNMLIIVLYVLIIFVATIISLHIAPTDFSVHQVVFDEVSAMSNVGIGVGYMTPGS
                     PIPIKWIFILLMWVGRLEIIPVLILIMGLVRGFEARVAGK"
BASE COUNT         1715 a         2044 c         2149 g         1363 t
ORIGIN      
        1 cccatccggg cgtgtaggcg ccgattacca ggaggacatc ggcctccctc gggttgttgt
       61 ggacgaagat accgtactgc tcgatatcat accgcggtgc caggcatgcc aggacctcga
      121 tgtcgcaccc gttgcaggag ccgacatcca cgtacgcgac gtggatggac ttcgacctga
      181 caaagttttt cagggactgc agaaccgtca tccggtgatc gcctccctga tcatctcctt
      241 tcccacccgc accgcctggt cgatcggcgt ccccccgcgg gccagttcct taaccctgtc
      301 gagtatctga agcatcctgt cctctccaag gagggggaca gcacgcgtaa agaggtgtgc
      361 tccaagctgc cgctcgggtt cgggagcctc ctcctgaacc ctcttcccct gctcgtagcg
      421 tgccgggagg ttctcgagga ggagcatgtc gcagcagtcc atgaggtagc gccgcatcct
      481 gttctggggg atgccgaatt cggcagcaat ctcgcgcacc atccggtcat ggcgccggct
      541 ttcgaggatt gcatacttca tggcgatgag gtctttctcg tagatcggtt ccctcatatc
      601 aggcctccca gcctcgcaag gccatacgcg acaaagagga ggactgctgc ccacaatgca
      661 atcgcctccc atgtccggag acgcagatcc tcccggcacc agtagacggt ggttgcgagg
      721 gcaagcgctg ccccaatggc caggccctgc aaccatagag caggacggtc gaaacactgc
      781 atggcgatta tcaggagata gatgagggtg ccaaccacca atacttctct gatcatgaca
      841 tcaccccgag gagggcaaca tatgcgacca tcaggccggc aatcagtgtc tggacagtca
      901 cactgtcgta tggtgagagc atgggagaga gggcgcacac gaacgagagt gtgatgagaa
      961 tgataaccat cgcgagaatg accagccaga acgggagatt cccgataaac acaaggatga
     1021 aggtcaggag gaggacatag gtcttcaggc cgttgcagac ctcgaggccc gccctccaca
     1081 cgccgaagtg ctcggtcttg tacccgctga cgagttcctt gctctccacg atcgagaatg
     1141 ggccaaacgg catcttggag aggaggacca cgtacatcgc cagcgccgcg agcgggacgg
     1201 tgatgagaag cggaccattg atctcctggt acgcgatgat atccgtaatc atcagcgact
     1261 gggtgaaaat tgcgatcgct gcgacgctga caaagagggg gatctcggaa gccgccgaga
     1321 tgaccgatcg gacccctccg aactttccat acggggaccc ggacgagagc ccgaacccat
     1381 gctcgacgat cttatggagc atgtagatgg caaagatgac caggaggctt cctcccgaga
     1441 gtaccacgac gagcgcagca gtccagatcc cgatggcgac caggactacg ccgcagaaga
     1501 gcgcggtgct cgcagtggcc ggaacccacg tggtcttgaa cgagaacttc agcatatgca
     1561 ggatctcctg ccacaccggg ggcccgggcc gcctctggat gcgggcaatc accttccggt
     1621 gaatgccaag caggaggagc ccgaagaaca cggcaaagag cagctcggcg atcattgcta
     1681 gtaccccagg aacggcatgt ccgcctcacc ctcgcgggcc atccagtaca gcccgagccc
     1741 cagcaggaaa agcgggaacc cggcaatcag gactgctgca taggcccaga aggggagaat
     1801 gccccagtaa ttgagagcca gtgctcctgc tgccgaaacc gccgaaacca gcatcagggc
     1861 aagggcatac tgcttgttca ttcgattcac ctcacaggag cagaatcacc tcggccactc
     1921 tcaggaagat caggagtgac gagagcgagc acatgatcac atagggggca ccgggtgccc
     1981 tgaacatctc ggccttggcc gcgtagaatg gggccatccc ctcccccatc accccgagca
     2041 cgagaagtgc ctttgcggca accgggatca ggatcatacc tttcgccagt tcgagcatcg
     2101 agagtgttcc tgttgttgca gcaatcatcg ccgccgcgga gaacatcggg acagtccccg
     2161 caagcgccac caggccgtac tggtaggcgg cattctggac atgcctgctc tttactgccg
     2221 ccacgatccc cacgttgcag atgccgacga gagagacaaa gagtgtgaaa tcaaagacat
     2281 cgccggtgac catcgccccc atgcacgcaa gaccgcaggc gatcgacatg aagcggcgga
     2341 accggagttc ggagagagag atctcctttg catagtacgc atccccgccg aatgcgatgt
     2401 cgatctggcg ctcgggcctg ctcaccagca caaacaccgt gaatacgagt gccaggatga
     2461 agagagtgag agtgaagggt gtgaagtaga tcacgatgtc cccgaactcc aggaagaaga
     2521 ggggtgctcc cccgatctcg ttcattcggg ttcaccccac atcgccccta cgagggacat
     2581 cttggcaatc accttcagga ggatcccgat tgcagcgccc atgagggcca tgtaccacca
     2641 ctggggggcg aacatgaaca cgaagaacgc cgcgacccag agcgcccagg agatcccgct
     2701 cgccatctcc agggcatgga agtccccgga gaagctcctg ctcatcagga ggaacacgat
     2761 gccgaggccc gcgatccccc cgccggtgaa ccctgagagg atgatgccgt acaccacaag
     2821 gatggcggag atgatgaccg gggccgggga ccgcatcatc acttcgatgt tcaggcgcgt
     2881 gacagggtgg tgctggtacc cttcctttgt caggtatatt tgcgagagtg ccagaagctc
     2941 ggctattccc accaccaggc cgggaagaat cagcgcctct gatagatcgg tcccgatcag
     3001 cgcgatcacc gcaagcgacg cggtctcgac gaggtcgatg agcaggagtt tatggaggtc
     3061 gtcccgttcc cagacgagag ccaggaaccc gatgagcgca catgcaaatg cggcggcaag
     3121 cccgatggag tattcgttcc acatcttttt ttaggcctcc ctcttcctga agatgtatgc
     3181 cgcgattgca aatgcaacaa acaggatggt cggttcaacg actgtatcaa gacccctcgt
     3241 attatagagg atctcatcga ggatgcctcc cgggtgtgcg acgatagtgg tccccatgta
     3301 ggcggtatgc tccgagatga agagagagag gggtgtaagg tatgccgtga cataccctgc
     3361 agtgggggag ttctccgggt actgggaatg gattgaaacc tgggtgaagg gcggcccgcc
     3421 cctgtcataa gggttgagcg tgctccccgg gacgatctgc tttggataca gctggtcttc
     3481 agaatatggc agcggtagcg agaggaggac gaagaccgac atcgccgcgc aggctgcggc
     3541 gaataccggc aggatattgt cgaagtttga gagaaccctc gatatcctct ggatgatcac
     3601 gccggcgcct cctttttctg gattactctc acgaagatat ataccgagag cgcagaaacc
     3661 cctccaaatg tcagaagcgc aagcgcctca ttgtaggaga gcatcagcag gaggagcccc
     3721 catgccgaga tctccaggtt gatcagccgg ttcaggtagg tttttggtac cggaaatgcc
     3781 gcagcgatgg ttccacccag caggagcaca agaccgagta tgaactccgg gttcattgat
     3841 ctcccctccc gcagaccatg aggatgaaga ttgtggagag cggggcgatg agcgcgaccg
     3901 cgatggcaac atcgaggagt cccctgtcaa cgatgaacgg gaccgatccc ccgacaagga
     3961 tgccgaggga gataagtttc ctgaagggat cacgctcgac gatggtgagc agggctccga
     4021 tgatggccag cgccccgaac gctatcaatg caatgttatt cacggcgatc cagccctcct
     4081 gcataggtac tggcgatggc gttcgactcg agtgtcgacc cgacgaagta ggccgcggcg
     4141 gcgacgatgc tcagcgggtg gggtatgagg agggcaatac tccccgcaac ggcaaaagag
     4201 agcacattca ggaaggggag tttcctgagt gtgtcaggct cgagcgcaac cctgatggct
     4261 gcgtacactg cgatgactgc gcacaccgcg agggcggctt cctgcggcgt catccttccc
     4321 tcaccttcca gaggtaggca aggaggcggc tggaatggcg gtgtgatatt ccctggatcg
     4381 tcaggatggc gatcaccatc cctgcgatga actcggcagg ggagaacccg atctgggaaa
     4441 agccccagac gatgaacgtt gcggtgatgg cgccggtagt gcctgcatac ccgcggtcat
     4501 ggcatatacg gttgccgatg aagaccagga atgctgcgag cagccctcct ggtatccctg
     4561 caatatagac cccgcaggag gcaaggagtg tccccgccga ggcgtcgggg gaagagacga
     4621 tgttgcccat catgtagccg ccgtggatgg cgcctccacc cctttcgatg tgtgtgccga
     4681 tggcggtcgc ccccaccacg cctccgcccg cagggagcct gaagaataga tctatcaata
     4741 tgaaattgag ccagcagacg gctgctgcaa ccaggatacg ggcgaatgcc ggaagaatgc
     4801 tatccatcgg gccgtcaacc tacaggtggt taggctgtga gaagaagaat attttgattt
     4861 tggtaaattg cgacgaatgg ggtgttgtaa ggttcctgat tggcacgcgg aggtggaaag
     4921 atcgagaatg tgcctgcatt agtcctggga tattcccttg tgtccttctg tacaaccgga
     4981 taatccatat tcccgcttcc gtggaaaaga aagcgttact ccctgctact tccctgccac
     5041 tcgtgcctca aagccccgga cgagccccat gatcaggatg aggaccggta tgatctcgag
     5101 cctcccgacc cacatcagga ggataaaaat ccacttgatc ggaatagggc ttcccggggt
     5161 catgtaccct acgccgattc ccacgttgct cattgccgag acctcgtcaa acacgacctg
     5221 gtgtacggaa aagtccgtcg gagcgatatg caggctgata atggtggcta cgaagatgat
     5281 caggacgtaa aggacgatga tcagcatgtt cttggagagc tcaacctcgg agatctgcct
     5341 ggggatggcc tttccctcgt gcctgaacgg gacaatcgcc ctccccctgg caaaaaagcg
     5401 cctgaaccac cagacaagcc cttcgtaccc gaggatgacg cggttggcct tgataccccc
     5461 ggcagtgctc ccggatgccc ccccgatcat catcaggaga gtgatgacga tgagcggtgc
     5521 tgcgacccag tggaggtcag aattctggag cccggtggat gtcaccgcgg atgcagtgca
     5581 gaacactccc tgtcgcacgg cggtttccag tgggaagctg ttgaagacat ggagatccag
     5641 agtgatcacg agcgacccga caaggatcag ggagaggatg agccatacga ccctgtcctg
     5701 gagaaaaggc ctgatcctcc ccctgtacat gaaaaaatag agcttaaatg ggagagcccc
     5761 tgcaatcatc accgggatca tgatggcctc aagaagcgca ttgtcataga acgagatccc
     5821 cgcgtcgtgg atggagaaac ccccggtgga aatgccgacc atcgccaggt tgagggcgtc
     5881 ccacagaggg atgccggaaa gcatcaccag ccccgtgaag aggatggtaa gaaagaggta
     5941 aatgacccac atccgtctcc cggtcgagac cacattcggc atgagagctt ctgacctgcc
     6001 ttcagaccgg tagagctggt gctgggtgag gcttgacctc gagagcatcg caataccaaa
     6061 ggcgattacc ccgatcccac ctatccactg catgaatgat cgatagaaaa gaagagtcct
     6121 tggcgtggtg tctaaggagc ggatcatcgt gatccctgaa tcggtccacc cggacatagc
     6181 ctcgaatata ctatcggtcc aggtcatgtg gaggccgaga acaaacggga ggctcccgac
     6241 cacggcaatg gcaagccata tgagggccac cgcggcaagc gcaaccgaga gctgtggtat
     6301 gtagtcccgc cttggaattc gggagatgag gagaccgagg atcacgaatg tcagcggcac
     6361 ggaggccatc ggcaccacca tgtcccactc cccgaagatg acgaggacaa ggagggggat
     6421 cagggtgaca aaacccagga actcgaagat cacccccatg tcatgggcga tcatcgccag
     6481 ggtctccagc cgcttcatga tcaggtcatt ggcggcagga gattatcttg attctcccct
     6541 ggtgcgcccg gtttggcagg aacacgacct cgatacctcg aaactgcggg ggcactgatt
     6601 gggttacaga tcacttgggg aagagccagg aaaaggggag tttttagagg gtcatatgag
     6661 tgcgacccgg gcatttggct gcacggaatg ccagggttcc gctcttttct gccataaaaa
     6721 gcagaaaaga taccatatat gaagttacaa aggcctggag ttcaggcctg cgagtgcatc
     6781 ccgtatgtcc tcatcatcca gtttggcaag aattttcgct cgtaggaggg aataatcgcc
     6841 atcgggcatg tcctgaccga aaggtggcga acggtcaggc gttttcacat gccctgtctt
     6901 ctccatgatg gcgtctctta ccatgttcct cacgcggttg gccgggatgc catgaccttg
     6961 ctggtgctga tctgacaagg tctgcggcct ggtatatgat cccagacctt catatatggc
     7021 acatttctat taaatctcca cgtatataat aattgcgcag aataatagtt ttgcgataga
     7081 tgaattggat tgaaagggac attacctccc gttctccgat ccattggata tgcaatgaga
     7141 accattcgcg caaaatgtga tcctggagac cccacggcat ggcaaagggt tttttcaccc
     7201 cccggatcct agtaagagga gagcgagggt tgccgagcgg tcaaaggcga tggatttagg
     7261 gtccattctc g
//
LOCUS       DAOQYR010000101         7016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_11121_length_7016_cov_8.856773, whole genome shotgun sequence.
ACCESSION   DAOQYR010000101 DAOQYR010000000
VERSION     DAOQYR010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11121_length_7016_cov_8.856773"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(487..1782)
                     /locus_tag="PKM87_10080"
     CDS             complement(487..1782)
                     /locus_tag="PKM87_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNQ30454.1"
                     /translation="MHRTAIPMLGVLLVISLLVAGCTSPEHPGPPVAGETPAPQSSIN
                     QSSPPLDAVVNANNRFAFDLYKSMAGNPEYGDGNIFFSPLSLSTALAITYEGARGQTA
                     DEIQSVFHFPENRTILRSGYTDLIARINDQADDCTLRTANALWAEKTHPFLPDYLGIA
                     DRTYGAKTTNLDFINAPDDSRVAINQWVEEKTEDRIKDLIPPGAIDSSTALVITNAIY
                     FKGTWEKQFDPVLTRDADFLSGNGDAVRVPMMQRTDDAAVFGYAETDKFQVLEMPYES
                     GSGTELSMLVVLPRENDTAGIAKSLDAGTLQEIRQSLAFRIVRVYFPKFVMETKYFLP
                     RTLSEMGMPRAFTSAADFSGMDGSRDLFITDVIHQAFVEVNEEGTEAAAATAVIMGKG
                     VAMVDDVPVFRADHPFIFFIQERETGNILFMGRVSDPVF"
     gene            complement(1876..3201)
                     /locus_tag="PKM87_10085"
     CDS             complement(1876..3201)
                     /locus_tag="PKM87_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNQ30455.1"
                     /translation="MNPSLAAIPALAAVALLAVILAGCTGPGEPGPSIEVTPTPNVTV
                     TTSPSPPNPALPGSATSVVDANNRFALDLYGRIADDPKAGDGNIFFSPFSISSALAIT
                     YEGARGTTAEEIRSVFHFPENQTVMRSGFAGIISGINSKANAYMLRTANALWAEQTYP
                     FLAGYISAAEQHYGAKTTNLDFINKPEESRVTINTWVEDLTEDRIKDLLQPGTIDPLT
                     RLVITNAIYFKGTWMKQFDQNKTTDADFRTGTGKTVRVRMMERTDEDAIYGYAETDIL
                     QALSMPYESGDGKDLSMLVLLPKTDDLSMVEESLNTSTLSLLHDSLKTRRVIVYFPKF
                     VMETKYSLPGVLSAMGMPTAFTRAADFSGMDGTKNLYIDDVIHQAFVEVNEEGTEAAA
                     ATAVVMRLSAMPLEETAPLFRADHPFIFLIQENETGNILFMGKVSDPSG"
     gene            3348..4145
                     /locus_tag="PKM87_10090"
     CDS             3348..4145
                     /locus_tag="PKM87_10090"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNQ30456.1"
                     /translation="MERYTWNPADYAEHSHAQYLWAGEVIAKLDLKGTESVLDIGCGD
                     GKVTALLASHLPSGEVIGIDSSAGMVNCARESFPGSDYPNISFLCMDAMDIRFSECFD
                     LVFSNATLHWVQDHATMLQGVYRCLKNRGRIFFQMGGRGNAREILDLAGSLIHSGRWR
                     RYFSDFRSPYTFLSAEEYQVLLRDAGFLPIRVELIPKDMTQVGCDGLCGWIRTTWLPY
                     TERIPEQQKNRFISDLAESYLEKYPPGSEGLIHVGMVRLEVEAQKPG"
     gene            complement(4206..4388)
                     /locus_tag="PKM87_10095"
     CDS             complement(4206..4388)
                     /locus_tag="PKM87_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HNQ30457.1"
                     /translation="MATDPVCYMVVDEDESEYRTSYGGQEYFFCSDFCRKKFEENPGR
                     YIKLARSLDIGPDISC"
     gene            4622..5302
                     /locus_tag="PKM87_10100"
     CDS             4622..5302
                     /locus_tag="PKM87_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HNQ30458.1"
                     /translation="MEFEQNVIDAFPGICVAEGDIRSVVVTRGSLELDCLKQEIAREI
                     TSRYTLENVKDDPLFRAYRDFFWSVGVDPTKTRPASEALVRRILSGGRLPRINTAVDA
                     YNLASAKSSVPIGAFDADTFDKNLTMRFAADGEQFIGIGMEKPLMLRPNQVVLTDSEQ
                     IIAVYPYRDSDATKVTTDTRDIHIVACGVPKVERGRVIEGYNLCAGYLERYAGGVASE
                     AEISPKVE"
     gene            complement(5370..5861)
                     /locus_tag="PKM87_10105"
     CDS             complement(5370..5861)
                     /locus_tag="PKM87_10105"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNQ30459.1"
                     /translation="MFRPPEGAKDTRPLLVIADSRGRIRCWDAIRRWPYMRDLLAFCS
                     SATPGEYLSYLEKQKVAAIVAGNDRIDMREALEMLNRQYGVETIQVDSGGTLNSVLLA
                     AGVVDEVSVLVHPFLAGGRADPTMFDPIKAGFPNLCVPLVHIWTEVVGNGLIWARYSV
                     EKS"
     gene            complement(5813..6052)
                     /locus_tag="PKM87_10110"
     CDS             complement(5813..6052)
                     /locus_tag="PKM87_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30460.1"
                     /translation="MMPRVIIHIATSLDGRITRFPADLELYYALAATWNPDAILFGSE
                     TVLTAVRENPALEVPPGTRRCSGHRRVQRTHARCS"
     gene            complement(6141..6368)
                     /locus_tag="PKM87_10115"
     CDS             complement(6141..6368)
                     /locus_tag="PKM87_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30461.1"
                     /translation="MEKKLDTCADCPDYNECRTIQDFFNKKGYKYGKYRQSLEFIRKF
                     GYRAFIESARSWKCAYGSLKPPEQEGKTAEY"
     gene            complement(6616..>7016)
                     /locus_tag="PKM87_10120"
     CDS             complement(6616..>7016)
                     /locus_tag="PKM87_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNQ30462.1"
                     /translation="SLSALRLEELNQALTYQRVMVSFPRFKMETTYSLSDTLSEMGMP
                     SAFRDADFSGMDGSRNLFIDEVYHKAYIDVNEEGTEAAAATAVVMNRSLAPGEPIPVF
                     RADHPFLFLIQDNDTGNILFMGRMVNPADV"
BASE COUNT         1655 a         1858 c         1811 g         1692 t
ORIGIN      
        1 ttatttagat tattcttact gagtcagtaa taaataatcg taattgaaca gaaaaatatc
       61 atccactgag cactctttca tacagaattg gttatatcgg gttatcgaaa atttttctgt
      121 tgtcaaatcg ccacatcaat agactagata ggaaaatact caaatatcga tgatgtcctc
      181 tttcatcaac caaatcattg aacggggacc agaacaaaat aaatcgcgtt tataggttgg
      241 taaaactggt cgatttcaga taccgatcat tttttttgca tcactcttca ccctaataat
      301 taattgaaca ttttcggaaa tttttatcga cagattcaat tgcccttttt cttcgtaaaa
      361 atgcgctatt ggatagtgag tctgttttgg aaaacctgcc gtttttttat aaaaaatccg
      421 gatgagtcaa atcccaccca tacaaagagg cagggtggat tctgcataag ctcaacagta
      481 aaaaaatcaa aagaccggat cgctcactcg tcccatgaag aggatattcc cggtttccct
      541 ctcctggatg aaaaagatga agggatgatc ggccctgaat acaggcacat catccaccat
      601 ggcgacacct ttacccatga tcaccgcagt cgccgctgcc gcctcagtcc cctcctcatt
      661 cacctctacg aatgcctggt ggatgacatc cgtaatgaag agatccctgc tcccgtccat
      721 cccggagaag tcggctgcac tggtaaaggc cctcggcatc cccatctccg aaagagtccg
      781 gggcaggaag tatttcgtct ccatcacaaa cttcgggaag tatacccgca caatcctaaa
      841 tgcaagtgac tgccggatct cctgcagggt tccagcgtca agcgatttcg cgatccctgc
      901 agtatcattc tctctgggaa ggacgaccag cattgagagt tctgtcccgc tgcccgactc
      961 gtagggcatc tcaaggacct ggaacttgtc ggtctctgca tacccgaata cggcagcatc
     1021 gtcggtccgc tgcatcatcg gaacgcggac cgcatctccg ttccctgaca ggaaatccgc
     1081 atccctcgtc agtaccgggt cgaactgttt ctcccacgtc cccttgaagt agatggcatt
     1141 cgtgatcacc agggcagtac tggaatcgat cgcgccgggt gggatcaggt ccttgattcg
     1201 atcctcggtc ttctcttcca cccactggtt gatggcgacc ctggaatcgt ctggtgcatt
     1261 gataaagtcg aggttagtcg tttttgcacc ataggtccga tctgcaattc cgaggtaatc
     1321 agggaggaag ggatgcgtct tctctgccca gagtgcgttt gccgtccgga gcgtgcagtc
     1381 gtccgcctgg tcattgatac gggcgatgag atcggtatat ccggaccgga gaatggtcct
     1441 gttttcgggg aaatggaaca cagactggat ctcgtcagcc gtctgtcccc tcgctccctc
     1501 gtatgtaatc gcaagggcgg ttgagagtga gagaggtgag aagaatatat tgccgtctcc
     1561 gtattccgga ttaccagcca ttgatttgta gagatcgaat gcaaaccggt tgtttgcatt
     1621 gaccaccgca tcaaggggcg gggacgactg attaatcgag gattgcgggg caggagtctc
     1681 cccggccacg ggaggacccg ggtgttccgg tgaggtacat cccgcaacga ggagcgaaat
     1741 gacaaggagt actcccagca ttgggatggc ggttctgtgc atgtcgatgt gtttggagat
     1801 acaatctgtt aagcctggcg ttatgtgcga aaattttctt cgtcataatt tccatcccca
     1861 aaaaattggc ggggatcagc cagagggatc gctcaccttc cccatgaaca ggatgttccc
     1921 cgtctcgttc tcctggatga gaaagatgaa gggatgatcg gcccggaaca acggcgctgt
     1981 ctcttccaac ggcattgcag aaagcctcat cacgactgcc gtggcggcgg cagcctccgt
     2041 cccctcctcg ttcacctcga caaatgcctg gtggatgaca tcgtcgatgt acaggttctt
     2101 cgtgccatcc atcccggaga agtcggccgc ccgggtgaac gcggtcggca ttcccattgc
     2161 agagagcaca ccgggcagtg agtacttggt ctccatcaca aacttcggga aatatacgat
     2221 gactctccgg gtcttaagcg aatcatgcag taaagagagc gtgctggtat tcaaggattc
     2281 ttcaaccatg gagagatcat cagtcttcgg gagaagcacc agcatcgaga gatctttccc
     2341 gtcgccggat tcgtatggca tcgagagcgc ctggagaatg tcggtctctg catagccata
     2401 gattgcatct tcgtcggttc gctccatcat ccggactcgc acagtctttc cggttccagt
     2461 ccggaaatct gcgtccgtgg tcttattctg atcaaactgc ttcatccatg tccccttgaa
     2521 ataaatggca ttggtgatca caagccgggt taaaggatcg attgtaccgg gctgcagcag
     2581 atctttgatc ctgtcttctg tcaggtcttc aacccacgta ttaatggtca ccctggactc
     2641 ctccggctta ttgatgaaat cgagattggt ggtctttgct ccatagtgct gctcagcagc
     2701 actgatatac ccggcaagga acggatatgt ctgttcagcc cagagagcgt tcgccgttcg
     2761 gagcatgtat gcattcgcct tgctgtttat gcccgaaata atcccggcga acccggatct
     2821 catgacagtc tggttctccg ggaaatgaaa taccgagcgt atctcttccg ctgtcgtccc
     2881 cctggctccc tcgtacgtga tggcaagcgc tgacgatatg gagaatggtg agaagaagat
     2941 gttgccgtca ccggcttttg gatcatctgc gatccttcca tacaggtcaa gggcaaaccg
     3001 gttgttcgca tcgacgacac ttgttgctga accaggaagt gcgggatttg ggggggaggg
     3061 ggatgtcgtc actgttacgt tgggagtggg agtcacctct atggacggcc cgggttctcc
     3121 cggccccgtg catccggcga gaatcactgc aaggagcgcg acagctgcca gcgcaggaat
     3181 ggctgcaagc gatggattca tgcacgtgtc tttttattca gataggttaa atctggcgtt
     3241 gagtgcggga aatttcggag ttatcaatgc agaagatgcc tgcacgtcca aacgttcttg
     3301 tcacctgctg caaaacatcc tgagtgaaat attccgtatt tgagaacatg gaacgatata
     3361 cgtggaaccc cgctgattat gcggagcatt cccatgccca gtacctgtgg gcaggggagg
     3421 tcatcgcaaa actcgatctg aaaggaacag aatcagtcct tgatattggg tgcggggacg
     3481 ggaaagtcac cgccctgctg gcctcccacc ttccgtcggg tgaggtgatc gggattgaca
     3541 gttccgccgg catggtgaac tgcgccaggg agtctttccc tggatcagat taccccaaca
     3601 tttcatttct ctgcatggat gcgatggata tccggttttc ggaatgcttc gacctcgttt
     3661 tttcaaatgc caccctccac tgggtgcagg accatgcgac gatgctccag ggagtgtatc
     3721 ggtgcctgaa gaaccgtggc aggatcttct tccagatggg tggcagggga aatgcaaggg
     3781 agatcctcga tttggccggt tcactgatcc attccggcag gtggcggaga tatttctcgg
     3841 atttcagaag cccctataca ttcctctctg cagaagaata ccaggtattg ctgagggatg
     3901 ccggctttct ccctatccgc gttgaactta tcccaaaaga catgacacag gtgggatgcg
     3961 acggcctttg cggctggata cgaacgactt ggcttcccta cacggaacga atccccgaac
     4021 aacaaaaaaa tcgtttcatt tccgatcttg cagaatcgta tcttgaaaaa tatcctcctg
     4081 gttcagaagg cctgattcac gtcgggatgg tccgcctgga agtggaagcc caaaaacccg
     4141 gataataatg cagatacccc cccggccccg acagtccact cccccaaaaa gtgaaacgga
     4201 aacattcaac aactgatgtc cggcccgata tccaatgatc gtgccagttt tatatacctt
     4261 ccggggttct cctcaaattt cttcctgcag aaatccgagc agaagaaata ttcctgccct
     4321 ccgtaggatg tccggtattc cgattcgtcc tcatcgacaa ccatgtagca gacaggatct
     4381 gtagccatgt atcatgcaca ccccctgtaa ttcgtgcatt ttacatcagg agacataaaa
     4441 atacttccat gccacccatt tcctgctcgt catatctccc agggaataat ggacaccagg
     4501 tcccggtttg ctcgaacacc cggagctttc ctgcagtatc ccccggcccg caaatgcgca
     4561 tccacaacgt ggacggaaaa gtatgtagtg tgaatacgca acacttttga ggaatattcg
     4621 catggaattt gaacagaacg taatcgacgc attccccgga atatgtgttg ccgagggtga
     4681 catcagatct gtggtggtca cccgcggcag tctggagctc gactgcctga aacaggagat
     4741 cgcccgggag atcacctccc gctataccct ggagaatgta aaggacgacc cactctttcg
     4801 ggcatatcgt gatttcttct ggagtgtcgg ggtcgatccc acgaagacaa ggcccgcctc
     4861 tgaggccctt gtgcgaagga tcctctccgg aggaagactc cccaggatca acacggcggt
     4921 ggatgcctac aacctcgcat cggcaaaaag cagcgttccc ataggggcgt tcgacgcaga
     4981 cacgtttgac aaaaacctca cgatgaggtt tgcagccgat ggcgaacaat tcatcgggat
     5041 cgggatggaa aagcctctca tgcttcgtcc aaaccaggtc gtcctcacag attcagaaca
     5101 gatcattgcg gtatatccct accgtgactc cgatgcaacg aaagtcacga ccgacacaag
     5161 agacatccat atcgtggcct gcggggtgcc gaaggtggag aggggaagag tgatcgaggg
     5221 ttacaatttg tgtgccggat acctagagcg atatgcgggc ggggttgctt cagaggcaga
     5281 gatttcaccc aaagtggaat gaccattcac tgcctgtatg aaggatcctg acttcattgt
     5341 atcatgcgat atctcctgaa aaaagagtat cagctttttt caaccgagta cctcgcccag
     5401 atgaggccat ttcctaccac ttccgtccaa atgtgaacaa gggggacgca aagattggga
     5461 aatcctgcct ttattgggtc gaacatggtc ggatcagccc tgcccccggc taggaaggga
     5521 tggacgagca cgctcacctc gtcgacaaca ccagccgcga ggagcacgct gttgagggtc
     5581 cccccgctgt cgacctggat ggtctcaacc ccgtactgcc tgttcagcat ctcgagggcc
     5641 tcccgcatat cgatccggtc gttgccggca acaatcgccg cgaccttctg tttttcgagg
     5701 taggagaggt attccccagg ggtcgcggat gaacagaacg cgagaagatc ccgcatgtac
     5761 ggccacctcc tgatcgcgtc ccagcagcgg atccgcccac ggctgtcggc gatcacgagc
     5821 agcgggcgtg tgtcctttgc accctccggt ggcctgaaca tctcctcgtg cccgggggga
     5881 cttcaagcgc aggattctcc cggacagccg tgaggactgt ctcgcttccg aacaggattg
     5941 catccgggtt ccaggtcgcc gccagggcat aatacagttc gagatcggcg ggaaatctcg
     6001 tgatccggcc atcgaggctg gttgcaatat gaatgatgac gcgaggcatc atggagatca
     6061 ctcttgtatc ataattcgga ttgattttat taaaggaacg gcagcgcagg gtgaaaatga
     6121 gaggagatgg agttttttaa tcaatactca gccgttttcc cctcctgttc tggaggtttg
     6181 aggctcccat aggcgcattt ccatgaacgg gctgattcaa taaatgcccg gtatccgaat
     6241 tttctgatga attcgaggga ttgccggtat ttaccgtatt tgtatccctt tttattgaaa
     6301 aaatcctgaa ttgtccgaca ttcattatag tcgggacaat ccgcacaggt atccagtttc
     6361 ttttccatga ggcagcaacg cttcattgca cagcgtgctg catggatatc ccttgtgcca
     6421 tcgtgatagc ccagtttgca gcccctgcac gcaccatcct tcaacgcgcg gcaggtaccg
     6481 caatacgcac cgcaacagcc gatcaggaca atctgtgaag tgggattgtt ctcgtgggca
     6541 tctgttttca tcatcttttg gaagagggaa tttggaatcc cggttggttc cccggtccca
     6601 tgctggttgg aaaggtcata catccgccgg gttcaccatg cgtcccatga agaggatatt
     6661 ccctgtatcg ttgtcctgga tcaggaacag gaaggggtga tccgcacgga agaccggaat
     6721 tggttcaccc ggcgcaagcg acctgttcat cacgactgcc gttgcagctg cggcctcagt
     6781 cccttcctcg ttgacatcaa tgtacgcctt gtggtatacc tcatcaatga ataaattcct
     6841 gctcccgtcc atccccgaga aatcggcatc tctaaacgca ctcggcatcc ccatttcgct
     6901 gagcgtatcc gagaggctgt atgtggtctc catcttgaac ctggggaagg ataccatcac
     6961 ccgctgatag gtaagtgcct ggttcaattc ttcaagcctc aaagcagaaa gagagg
//
LOCUS       DAOQYR010000102         6803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_11571_length_6803_cov_8.179905, whole genome shotgun sequence.
ACCESSION   DAOQYR010000102 DAOQYR010000000
VERSION     DAOQYR010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11571_length_6803_cov_8.179905"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..878
                     /locus_tag="PKM87_10125"
     CDS             21..878
                     /locus_tag="PKM87_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HNQ30463.1"
                     /translation="MAISADHIRQLHPYEIRILHTLERLMRTHAWVPLELIKKSTGFS
                     ESETLFRLGRLMERGMVRYDVVPYEGYSLIFSGYDTLALLSLTRRGTISALGPQIGEG
                     KESVVYEGLGLGCVALKFHHVGQRSFQSVRLSREYLAEEGHCPWIFASRLSAEREHEA
                     LKILHPRVRVPLPIDRNRNVVAMELIQGVNMNRCSLADPAGVMDSILAAVGEAYRLGV
                     IHADLSEFNVMIDGNEVVLIDWPQWISPDHPNAGEILARDVGNLVRYFSRKYRISTDL
                     ETAIERVTG"
     gene            875..2827
                     /locus_tag="PKM87_10130"
     CDS             875..2827
                     /locus_tag="PKM87_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF460 domain-containing protein"
                     /protein_id="HNQ30464.1"
                     /translation="MKIFGIDVIKGSVRSRSRRPMYALVILHEREIVSESEVSGFRLL
                     RLLNAERPDILAVDSLQEIAVDQHDLFSFLQALPPSVRLVQVTGGERKETLGKVASRF
                     NISFNRFDPFDEARTIARVAALGAGAEVVAFENESEIVVSRHRSPGKGGWSQNRYVRK
                     MHGAVQAKAREIGMELMAAGLRYEKKETRAFGGCSRVSFTVRAPRDQIPVSTFRGADV
                     QVRISGKRLERIRFRPLSSKPPYLIAGIDPGTTTAIAALDLDGTLLLLQSSRQISMSG
                     VIEALYRVGKPLIVASDVHDMPFSVEKIRRAFNAVAHTPRQDMSVEAKQTLTSGYSCQ
                     NDHERDALSAALEAYRTYRNKFQSLQKRVPPGYELEEVRAGIVRGQSVEQVIGDLNEK
                     AAPTEEKAPAVEMDAARDERIRILDGTIKRLRILVGELQEDLRERDLQVMRLQERLKR
                     VRSRQDQKVRADAEVTKRDVIIQNLKKRLRKEERTTRAMKRRVERMKEFDEALLSGET
                     IPLKILPALTREAIKTFSAEMGIREGDVLYVSRIEGFGKSVAKDLSQEGIAALLVGEF
                     AGKAVAMEVEAVFRELDIPLIAADQVRVVVKGGRGAADPGQFRDAVKRWKELQDRYEK
                     EKKGRMLEDIFREYRAERGKEMKKVG"
     gene            2820..3677
                     /gene="thiL"
                     /locus_tag="PKM87_10135"
     CDS             2820..3677
                     /gene="thiL"
                     /locus_tag="PKM87_10135"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNQ30465.1"
                     /translation="MDERALKRVVMEVVGRDAVLDDCAILPLDDRFLVISTDMLHAST
                     DFPEQMTDWQVGWMSVAVTLSDIAAMGADPADILLAVGLDDPSRLRPILEGAKACCDA
                     YGARFAGGDLDSHRELTLVSSGTGFTRKPVRRAGSKVGDVVGIVGVPGRAQAALEGYP
                     KFQQYLFTPRPRVEEGRALAAAGATSMMDVSDGLLLSLFDMREANPCGYEIFTHKIPT
                     IEGIPQDLALDLALRGGGDFGLLFTCRPEVLPLPLPELYVIGNVIEEPAVMIDGKPAE
                     AKGYQHQWD"
     gene            complement(3679..5664)
                     /locus_tag="PKM87_10140"
     CDS             complement(3679..5664)
                     /locus_tag="PKM87_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ30466.1"
                     /translation="MKISPWLLFLLALVCASLAGCISPQSGDLLEEARALQQTGNLNQ
                     SLTKYDDAVSLDANNSAAWAGRGDVLHLMGRDEDALHSYNQSLEIGPASVQTWIAMGD
                     ILAGMGRNEEAFNAYDRAIGLDSNATMAWNGVGEVWAREGRYKDSAAAFSMVLRTNGS
                     YSPAAKKLGDVLVALKEYDQALRSYDYALNVTPSYGEAALAKGDLLSQFGKYQEALAA
                     YEYALMYLPNNTVLISHLASMQTSLGKYQDAVATYDRAIALEPQNATFWAYKSYLLNN
                     LGRFNESLAAADEAIALDPHNDVAWNNKGFSYNNLKRYEDALSAYSSALNATPDNVAA
                     LTNRGYAYLNLGEFQSAISDLDRAISLEPRFSSAWAYRGLAYLRMGMFDEAIRDATRA
                     AELDPRNAHAWSTQGMALLQKGKYRNASTSFNQALAITQTSPDIWLNQGLALLGAKDY
                     QGAIDAYDQVLSIDPANSAAWAYKVTALQNLGRGEEAVLITNNELKKDPDSLISLIKK
                     AYALSALKRLDEALVIYNRVLERDPFNLDVLLQKGILQLDLGDYLGAIKTYDKVMEID
                     SNNTDALMYQGIAYYRSGQYERSIENYERMIALNPGVSMAWSSRGYCLIKLGRIQDAI
                     KSFDKALELYPGNSDAVYGRVEAIRVMWPSYTPAWDR"
     gene            complement(5810..6757)
                     /locus_tag="PKM87_10145"
     CDS             complement(5810..6757)
                     /locus_tag="PKM87_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30467.1"
                     /translation="MKKIGIAVLALLILFSGMAMADDPVGATRQTQGITTVTHVVVYG
                     TFTDSAEAVWVSSNQDLRNNPPLNNYSDELDANGDGIIDDPLTGDQAWTPEAQYTMSY
                     SEQTLADNGYIEWDKTVSLDTGNKVANQDNFKATTQFDFVSFEDAFGRATFSESLMLD
                     AASMGSDAGSRMLCPFSTGDNGYIPAFCNIVEMGSSFTGSQVSMVTQANERHVSASAD
                     APAAMSYSVGLSGVGSAAAWINAHIMEGRTGGVFGTYTAPDGVTYEPGFWNYDTGSGS
                     PDNGFMQGVDMVYKEKTTASGVIESFSKSMSYQSGMRRI"
BASE COUNT         1447 a         1851 c         2053 g         1452 t
ORIGIN      
        1 agataaattg tgtagcacac atggcaattt ctgccgacca tatccggcag ctccatcctt
       61 acgagatccg cattctgcat accctggagc gcctgatgag gacccatgca tgggtccccc
      121 tcgagctgat aaaaaaatcg acgggtttct ccgaatcgga aacgctcttt cgcctcggcc
      181 gtctcatgga gaggggcatg gtgcgctacg acgtggtgcc gtacgaggga tacagcctca
      241 tcttctctgg ctacgacaca ctggccctcc tctccctcac caggaggggg actatatcgg
      301 cacttggccc gcagatcggc gaagggaagg agtccgtggt atacgagggg ttaggtctcg
      361 gctgcgttgc gctcaagttc caccacgtcg ggcagcggtc gttccagtca gtccggctct
      421 cccgcgagta cctcgccgaa gaggggcatt gcccgtggat ctttgcatcg cggctctctg
      481 cggaacggga acacgaggcc ttaaagatac tccatccccg tgtccgggtg ccgctcccca
      541 tagaccggaa caggaacgtg gtggccatgg aattgatcca gggggtgaac atgaaccgct
      601 gcagcctcgc ggatccggca ggtgtcatgg actccatact ggcagcggtg ggggaggcgt
      661 accgcctcgg cgtcatccat gcggacctct cggagttcaa cgtgatgatc gacggcaacg
      721 aggtggtcct tatcgactgg ccgcagtgga tatcccccga tcacccgaac gcgggggaga
      781 tactcgcgag ggacgtggga aacctcgtca ggtacttctc gcgtaagtac cgtatcagca
      841 ccgatctgga gactgccatt gagagagtga ccggatgaag atctttggca tcgatgtgat
      901 caagggttcg gtgaggtcga ggtcgaggcg tcctatgtat gccctcgtga tcctgcacga
      961 acgcgagatc gtcagcgagt ccgaggtctc gggctttcgc ctcctgcgcc tgctgaacgc
     1021 ggaacgccct gatatccttg ccgttgacag cctgcaggag atcgccgtgg accagcacga
     1081 cctcttctcg ttcctccagg cactcccccc ctcggtcagg ttggtccagg tgacgggtgg
     1141 cgagaggaaa gagacgctcg ggaaggtcgc gagcaggttc aacatcagct tcaaccggtt
     1201 cgaccccttt gacgaggcca ggacgattgc gagggtggca gcccttgggg cgggtgcaga
     1261 ggtcgtcgcg ttcgagaacg agagcgagat cgtggtcagc aggcaccgct ctccgggaaa
     1321 gggcgggtgg agccagaacc ggtatgtccg gaagatgcac ggggcggtcc aggcgaaggc
     1381 aagggagatc ggaatggagc tgatggcggc aggcctccgg tacgagaaga aggagacgcg
     1441 ggcgtttgga gggtgcagca gggtctcttt cacagtccgg gctccaaggg accagatccc
     1501 ggtctcaacg ttccgggggg cggacgtcca ggtgcgcatt tcgggaaaga ggctcgagcg
     1561 gatacggttc cgcccgctct ccagcaagcc cccctacctg attgcaggga tcgacccagg
     1621 gacgaccacg gccatcgcag ccctcgacct cgacggaacc ctcctcctgc tgcagagctc
     1681 ccgccagatc tccatgtccg gggttatcga ggcgctttac cgggtgggaa agcccctgat
     1741 cgtcgcctcc gacgtgcatg atatgccgtt ctcggtcgag aagatacgaa gggccttcaa
     1801 cgcggtggca cataccccaa ggcaggacat gagcgtggag gcaaagcaga ctctcacgtc
     1861 aggatattcc tgccagaacg accacgagcg ggatgcgctc tcggcggctc ttgaggcata
     1921 ccggacgtac aggaacaagt tccagagcct ccagaagagg gtcccgccag ggtatgaact
     1981 cgaggaggtg agggccggga tcgtccgcgg ccagtcagtc gagcaggtga tcggggacct
     2041 gaatgagaag gcggcaccaa ccgaagagaa ggccccggcc gtcgagatgg atgctgcgcg
     2101 tgacgagcgg atccgtatcc tggacgggac gataaaaagg ctccggatcc ttgtcggcga
     2161 actccaggaa gacctccgcg agagagacct gcaggtgatg cggctccagg agaggctgaa
     2221 gagagtgcgt tcccggcagg accagaaggt ccgggcggat gccgaggtga caaagagaga
     2281 cgtaatcatc cagaacctca aaaaaaggct ccgcaaggag gagaggacca cccgtgccat
     2341 gaagagacgc gtagagagga tgaaggagtt tgacgaagcc cttctgtccg gagagaccat
     2401 tccactcaag atcctgcctg cactcacaag ggaagcaatc aaaaccttct ctgcggagat
     2461 gggaatccgg gaaggggatg tcctctacgt gtcccgcatc gagggttttg ggaagagcgt
     2521 cgcaaaagac ctctcacagg aaggaattgc ggccctgctt gtcggggagt tcgctggtaa
     2581 agccgttgcc atggaggtgg aggcagtatt ccgcgaactg gatatcccgc tcattgcggc
     2641 agaccaggta agggtggtgg tcaaaggggg aaggggtgcc gcggaccccg ggcagttcag
     2701 ggacgcagtc aagcggtgga aggagttgca ggatcgctac gaaaaggaga aaaaaggaag
     2761 gatgcttgag gatatcttca gagaatatcg tgccgagcgg ggaaaggaga tgaagaaggt
     2821 tggatgagcg cgccctgaag cgggtggtca tggaggtcgt gggcagggac gcggttctcg
     2881 acgactgcgc catcctcccc ctcgatgatc ggttcctcgt catatcaacc gacatgctgc
     2941 atgcgagcac cgattttcct gaacaaatga ccgactggca ggtgggatgg atgagcgtgg
     3001 cagtgacgct ctcggacatc gcggcaatgg gtgcggaccc ggcagacatc ctccttgcgg
     3061 tgggactcga cgacccatcg cggctacgcc ccattctgga aggggcaaag gcttgctgtg
     3121 atgcatacgg cgctcgtttt gcggggggag atcttgattc ccaccgggag ctcaccctcg
     3181 tcagctcggg gacagggttc acccggaaac ccgtacgccg ggctggatca aaggttggtg
     3241 acgtggtcgg aatcgtgggg gttcccggca gggcccaggc agcgctggag ggttatccca
     3301 agtttcagca gtatctcttc accccacgac cgagggtgga agagggacgg gcactcgccg
     3361 ctgcaggtgc cacctcgatg atggacgtga gcgacggcct tctcctgtcc ctctttgaca
     3421 tgcgggaggc aaacccctgc gggtacgaga tcttcacgca caagattcct acaattgaag
     3481 gtatccccca ggatcttgca ctggatcttg ctcttcgagg gggaggggac ttcgggctcc
     3541 ttttcacctg cagacctgaa gtccttcccc tgcccctccc cgaactgtat gtgattggca
     3601 atgtcatcga agagcctgcc gtgatgatag acggcaagcc tgcggaggcc aaggggtacc
     3661 agcaccaatg ggattagatt atctgtccca tgcaggagta tacgagggcc acatcacgcg
     3721 tatcgcttct acccggccgt acacagcatc gctattcccg ggatataatt cgagtgcctt
     3781 gtcaaatgac ttgattgcgt cctggatgcg gccgagcttg atgaggcaat acccgcgact
     3841 agaccatgcc atggagaccc ctggattgag tgcgatcata cgctcatagt tctcaatcga
     3901 tctctcatac tgccctgacc tgtaataggc gatgccctgg tacatgagtg catcggtgtt
     3961 gttggaatcg atctccatca ccttgtcata agtctttatg gcaccgagat aatctccgag
     4021 atcgagctgg agtatcccct tttgcaacag cacatcaaga ttgaagggat ccctctcaag
     4081 cacccggtta tagatgacta acgcctcgtc gagacgtttc aatgcagaga gtgcataggc
     4141 cttcttgatc agcgaaatca ggctatccgg atcttttttg agttcgttgt tcgtgatgag
     4201 gactgcctct tcccctcttc ccaggttctg cagagcggtg accttgtagg cccacgcggc
     4261 gctgttcgca gggtctatcg agaggacctg gtcataggca tctattgcac cctggtaatc
     4321 ctttgcaccc aggagtgcaa gtccctggtt caaccatata tcaggggagg tctgggtaat
     4381 tgcgagcgcc tggttgaacg aggtggatgc attccggtat tttcccttct ggagaagtgc
     4441 catcccctgg gtcgaccatg catgcgcatt ccgggggtcg agttcggccg cccgggtggc
     4501 gtccctgatc gcctcgtcga acatgcccat ccggaggtat gccagaccgc gataggccca
     4561 cgcggaagag aacctgggct caagggagat ggcgcggtcg aggtcgctga tcgcagactg
     4621 gaattctccg aggttcaggt atgcatatcc gcgattggtg agagccgcga cattgtccgg
     4681 ggttgcgttg agtgcagagg aatatgccga taatgcgtcc tcatagcgct tcaggttgtt
     4741 gtatgaaaag cccttgttgt tccatgccac gtcattgtgt gggtcaagtg caatcgcttc
     4801 gtccgctgca gcaagagatt cattgaatct tccgaggttg ttcaggagat aactcttgta
     4861 agcccagaat gttgcattct gtggttcgag tgcaatcgcc ctgtcatagg tcgcgacagc
     4921 gtcctggtat tttcccagag aggtctgcat ggatgccaag tgcgaaataa gcaccgtatt
     4981 gttgggcaga tacatgaggg catactcata cgcagcgagt gcttcctggt actttccgaa
     5041 ctgggagaga aggtcccctt tcgcaagcgc agcctcgcca tacgaaggcg tgacattcag
     5101 ggcatagtca tacgagcgaa gggcctggtc gtactctttt aatgcgacga gcacatcgcc
     5161 gagcttcttt gcggccggtg aataggaacc atttgtccgg aggaccatcg agaacgcggc
     5221 agcagagtcc ttgtatctcc cttcgcgggc ccagacttcg cctactccgt tccatgccat
     5281 cgtggcgttg ctgtcaaggc ctattgcgcg atcgtatgca ttgaatgcct cctcgttcct
     5341 tcccatgccg gcaaggatat cacccatggc aatccaggtc tggactgaag cgggtccaat
     5401 ttccagtgac tggttgtaag aatggagtgc atcctcgtct cttcccatga ggtgcaggac
     5461 gtcacccctt cctgcccagg cagcactgtt gttggcatcc agggatacgg cgtcgtcata
     5521 ttttgtgagg gactggttga ggttccctgt ctgctgtaat gccctggcct cttcaaggag
     5581 atctccggat tgcggggata tgcacccggc aagcgatgcg cataccaggg ccaggaggaa
     5641 taagagccag ggactgatct tcattcttaa aaattcgcca tgaaggttct taacatctgt
     5701 ggaacctgcc agtacccctg gatatctcaa aggttcaacg aaggactatc caatggaaaa
     5761 aaatttatgg ggcatttttc tgccccatac catgccggtt tttcaggtct caaatcctgc
     5821 gcattccgga ctggtagctc atcgacttgc tgaatgattc gatgacgccg gaagcggtgg
     5881 tcttctcttt gtataccatg tcgacgccct gcatgaagcc attgtctgga gagccggagc
     5941 ccgtgtcgta attccagaac ccgggttcat aagtaacgcc gtcaggtgcg gtataggtgc
     6001 cgaacactcc gccggtcctg ccttccataa tatgggcatt gatccaggca gcggcagatc
     6061 ccactccaga taggccaacg ctgtaggaca tggcagcagg ggcatctgca gatgctgaca
     6121 catggcgttc gttggcctgg gtgaccatcg atacctggga acctgtgaag gagcttccca
     6181 tctcgactat gttgcagaag gcagggatgt agccattgtc tccggtggag aacggacaca
     6241 gcatgcggct gccggcatcc gatcccatgc ttgctgcatc aagcatcaga ctctccgaga
     6301 atgtcgctct tccaaaggca tcttcaaaag acacgaaatc gaattgcgtg gtcgccttga
     6361 agttgtcctg gtttgcgact ttatttccgg tatcgaggct cacggtcttg tcccactcta
     6421 tatagccgtt gtccgccagg gtctgttcgc tgtaggacat ggtatactgg gcttcagggg
     6481 tccaggcctg gtctcctgtc aatggatcat ctattatccc atcaccattt gcgtccagct
     6541 catcgctgta attattcagc ggcgggttgt tacgcaggtc ctggttcgac gacacccaga
     6601 ctgcctcggc actgtcagta aacgtgccat agacaaccac gtgggtaact gtggtgattc
     6661 cctgtgtctg cctggttgcg ccaacagggt catctgccat tgccatccct gaaaagagga
     6721 tgagtaatgc cagcacagca attccgattt ttttcattga tcatctcctg tagttcggtt
     6781 ccgatactca cagcatcaga att
//
LOCUS       DAOQYR010000103         6760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_11655_length_6760_cov_8.148397, whole genome shotgun sequence.
ACCESSION   DAOQYR010000103 DAOQYR010000000
VERSION     DAOQYR010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11655_length_6760_cov_8.148397"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            504..1262
                     /locus_tag="PKM87_10150"
     CDS             504..1262
                     /locus_tag="PKM87_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ30468.1"
                     /translation="MDDEPALFPVTKLALEKDGDVQVDLCVSPADARTLLEKRHYQVI
                     ISDYDMPGEDGITFLRHLRESGDDTPFIIFTGKGNETVAMEACNAGVDYYLTKSGSPR
                     EIFCRLKECVMRASWGYRLRHPWDIAFTTPLGVLETLPMPVFVLDERLHIRYLNRPAT
                     RISGMKTHDLELLSFNSLLEKNSSRELIVFLEHVIGGSESEDDATCPVPLVFMGRDGR
                     CIQMECSILPARDENGRPTGFVVTCKERMLRDPG"
     gene            complement(1283..3355)
                     /locus_tag="PKM87_10155"
     CDS             complement(1283..3355)
                     /locus_tag="PKM87_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNQ30469.1"
                     /translation="MKEEKRDSGSPRLLTEQEGMELLARAKIPVPEHGIASSPDEAGE
                     IAGSIGFPVVMKVVSPQVVHKSDAGGVVTGIRSVGEAREAFSRISRAVMEKVPGAIIT
                     GIMVEKECPPGFEIIVGGKTDPAFGKVITLGTGGTLVEFLRDVSIRVLPIDRAGVSEM
                     IDETRGSRIIQGFRGSPALDKGVLVETVLRLAHLFEHNSDWHEFDINPLILYPHGCVA
                     VDARIYLSVRSEKEGIVQIPSPDPAIFSPRSVAVIGASSDPRKIGYVVLRNLLQFPGP
                     VYPVNPKSPEILGKTAYSRVTEIPGDLDAAVIAIPAPAVPGVLRQCTEKGVKLAIILS
                     SGFRESGDEGAALEKEILEIARAGEMRIVGPNCLGIIVPHANLNTTFDPVSPMNGHIG
                     FISQSGAVVTTIVDWSIPEYIGFSVVISVGNQVDLGFVDFLRVVAADPHTRAIILYIE
                     EIRRGREFLSAVKEITAKKPVIALKSGSSRKGQKAAASHTGSLAGNYQVYQAAFRQTG
                     IIPVYTIQEAFDVAELLASEGYPKGNRALVITTAGGFAVMASDYAEWYGIALPPLTDE
                     MVTALDEFLPPMWSRENPLDIIGDGGAERYARVFDVLTRFQDEWDIAVVIAVPSAILD
                     PSHLAQEIARFSRNTHKMVIGCMLGGDTMRSGERVLRERHIPNYREIDGAFRAVGRAL
                     SARPQQKE"
     gene            3481..5037
                     /locus_tag="PKM87_10160"
     CDS             3481..5037
                     /locus_tag="PKM87_10160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ30470.1"
                     /translation="MPLRELEEALDAFANAGEPFLLSEVLARAGKKGHILHTAEALWP
                     MFTTLDLVAERVPGDLRLAKRPSSPQKTLTLDERRRLDAFFRKPELPPGIEACIQEYV
                     HAKTGKAWNDPSVLQKIRRAIVSQKDEYWREGEKKRVMYQKGYDVLGYLAYQAPVYFI
                     QAMHLLYDLSLEGCLKQHMRVLDLGCGPGVVPLAISSFLERIGGCSAEIYSVEKSEEF
                     IEAFRFLADHVPAPRGSVTLHPPLNADLVTVPLSCLPPSLDLIVLQNVLNEMSGHSPR
                     EKGRLVCRFAEALSPEGSILLVEPADMVNSMDLRVVVHHATGSGLFLHAPCRFLWNTQ
                     CTPDRCWSFVEKPPIHATGLMEALASGEEGYRYCNTDIKYSFALLRKVPPNPVPGLPL
                     SPRNYARLSALHRHIKRRINVAGVVMSGNLGDHKTYVIKICDGTTKKPVYAILPSYHL
                     SHENSALLSIPYGSIAEFTSVLARFNEKHHAYNLLVSRESRVKEFPAQSRAADWNLTG
                     PREKRNKLKK"
     gene            5340..6236
                     /gene="pyrB"
                     /locus_tag="PKM87_10165"
     CDS             5340..6236
                     /gene="pyrB"
                     /locus_tag="PKM87_10165"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNQ30471.1"
                     /translation="MRHIISIRDLDRAEINGILDHAQALQEQDYDPQALTGKLLGLLF
                     FEPSTRTRMSFASAVARLGGHSISMDSVEASSMVKGETLADTIRVVSGYVDAIVLRHP
                     KEGAARLASEFAQVPVINAGDGAGQHPSQTLLDLYTIRQSMPLEGVTVGLLGDLRYGR
                     TAHSLAHALSLYRATLHTISPKGLELPSGLAGELRDSGTEVIEHDDVQEVVKDLDVLY
                     VTRIQRERFPDSSSYFKVASSYRVTPELLEGVRDHLVVLHPLPRVNEIDPRVDHLPYA
                     RYFEQSANGIPVRMAMLLRVME"
     gene            6233..6703
                     /gene="pyrI"
                     /locus_tag="PKM87_10170"
     CDS             6233..6703
                     /gene="pyrI"
                     /locus_tag="PKM87_10170"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HNQ30472.1"
                     /translation="MKKGKNAPKGLLISPIENGTVIDHITAGEALNVLRIIGITGTTQ
                     ESISVATNVESGKIGFKDIVKIKNRELRKEEVDKIALIAPRASINIIRNYKVWEKKGV
                     EIPSEVIGIVRCPNPGCISNTNEPIESCFLVQPKGLRCVYCDWLISKDITSHII"
BASE COUNT         1464 a         1985 c         1855 g         1456 t
ORIGIN      
        1 gtgtgtgcaa gtttttcagg atctggcatg cgctacaagt gtccaattgc cgatatggtg
       61 tgatgcctca atgatgtgtg cattcctctg acagattggt ggatgattcg gggtggtgtt
      121 gcatgctgtc ccatgcctcc tcgcaaggga cagcggggag gggggactcc gcggcacatt
      181 agggacgggc gtcgtgcctc gccggcgagc gggatgaaca tgattccttt caccgccaca
      241 acctatcccg tcaccggtcg cccctgcttc ggggtggctg tccccctgcc tcacttccat
      301 gcctcaccac accatagtgc cgcggttctt catttaaatt ggacacatta agaaggtact
      361 atctccctca ctggtatttc aatgatagaa ccgggaggtt caacaagaag ctgagtcact
      421 gtgagaatta tgatgattcc tgagagacta ttgtctgtat cagggatgca ggcgaaggcg
      481 atctattgat ccacgttctc cttgtggatg atgaaccagc actctttcct gtgacgaaac
      541 tggcgctgga gaaggatggc gatgtccagg ttgacctttg cgtatccccg gcggatgcca
      601 ggaccctcct ggagaaaagg cactatcagg tgatcatctc agactacgat atgcccggcg
      661 aggatggcat cacctttctc cgccatctcc gggagtcggg tgacgatacg ccgttcatca
      721 tcttcacagg aaagggaaat gagacggtag cgatggaagc atgcaatgcc ggagtagact
      781 attatctcac caagtctggt tccccgcgag aaatattttg ccgcctgaag gaatgcgtga
      841 tgcgcgcctc ctggggatat cgtctgcgac acccctggga tatcgcattc acgacaccgc
      901 tgggagtgct cgagaccctt cccatgccgg tcttcgttct tgatgagagg ctgcatatcc
      961 gttacctgaa ccggcctgcc accaggatat ccggaatgaa aacccatgac ctggaacttc
     1021 tttccttcaa cagtcttctc gagaaaaatt cgtccaggga attgattgtt tttctcgaac
     1081 atgtgatagg aggcagcgaa agcgaggatg atgccacgtg cccggttccc cttgtattca
     1141 tgggcaggga tggcagatgt atccagatgg aatgtagtat tctcccggcc cgcgacgaga
     1201 acggccgtcc caccggattc gtcgtgacct gcaaggagcg catgttgcgg gatcccgggt
     1261 agtccagttg gggatcccga agtcattcct tttgttgcgg gcgtgcagag agcgctctcc
     1321 ccactgccct gaacgccccg tcaatttcgc ggtaatttgg gatatgccgc tctctcaaca
     1381 ctcgttctcc actcctcatg gtgtcgcccc cgagcatgca accgatcacc atcttgtgtg
     1441 tgttcctgga gaagcgggca atctcctgcg ccaggtggga gggatcaagg atggctgatg
     1501 gaaccgcaat caccaccgca atgtcccatt cgtcctggaa acgggtcaac acatcaaaga
     1561 cccttgcata cctctccgcc ccaccgtcgc cgatgatgtc gagcgggttc tcccggctcc
     1621 acattggggg gaggaactca tccagtgccg ttaccatctc atccgtgaga ggtggaagtg
     1681 cgatgccgta ccactctgca taatcagacg ccatcacggc aaagccaccc gcggtggtga
     1741 tcacgagggc cctgttcccc ttcgggtatc cttcagaggc caggagttcg gccacgtcga
     1801 acgcttcctg gatggtgtac accgggataa tcccggtctg gcggaacgct gcctggtaca
     1861 cctggtaatt tcctgcgagt gacccggtgt gcgaggcggc agccttctgc ccctttctcg
     1921 aagatcctga cttgagcgca atgaccggtt ttttcgcggt aatctccttc accgcagaga
     1981 ggaactctct cccgcgtcgt atctcttcga tgtagaggat gatcgcccgg gtatgtggat
     2041 cggcagccac cacgcggaga aagtcgacga acccgagatc gacctggttg cccacgctga
     2101 ttacgacgga aaagccgata tactcaggga tgctccagtc cacgatggtg gtgacgaccg
     2161 ccccgctctg cgagatgaac ccgatatggc cgttcatagg tgagacagga tcaaaagtgg
     2221 tgttgaggtt tgcgtgtgga acaatgattc caaggcaatt tgggcccacg atacgcattt
     2281 cccctgccct tgcgatctcc aggatctcct tctcgagcgc tgcgccttca tctccactct
     2341 cgcggaatcc tgatgagagg atgatggcca gcttcacccc tttctctgta cattggcgaa
     2401 gtacccctgg gaccgcggga gccgggatgg cgatgaccgc agcatccagg tcgccgggaa
     2461 tctccgtgac gcgggagtat gccgtcttcc cgagtatctc cgggcttttc ggattgacag
     2521 ggtacacagg tccggggaac tggaggaggt ttcgcaggac cacatatcca atctttcgcg
     2581 gatcggatga ggcgccgatg acggcaacgg agcgtgggga aaagattgcc gggtccgggg
     2641 aagggatttg cacaatacct tccttttctg atcggacgga caggtagatg cgggcatcca
     2701 ctgccacgca accgtggggg tacagtatca gggggttgat atcgaactcg tgccagtcgg
     2761 aattgtgctc gaagaggtgt gcaaggcgaa ggacagtctc gacaagtact cctttgtcca
     2821 gggctggaga tcctctgaac ccctgtatga tacgggaccc ccgggtttca tcgatcatct
     2881 ccgaaacgcc tgccctgtcg atgggaagca ccctgatgga gacatcgcga aggaactcga
     2941 cgagggtgcc cccggtgccc aacgtgatca ccttcccaaa ggcaggatct gttttccccc
     3001 ccacaatgat ctcgaaaccc ggtgggcact ctttctcgac cattatccct gtgatgatcg
     3061 caccggggac tttctccatg actgcacggg atatccggct gaacgcctcc ctggcctccc
     3121 cgacagacct gatgccggtg acaacccccc cggcatcact cttgtgcacc acctggggcg
     3181 atacgacttt catcaccacc ggaaatccaa tcgacccggc aatctctcct gcctcatcgg
     3241 gggatgatgc gatcccatgt tccgggaccg ggatcttcgc ccgggcaagg agttccatcc
     3301 cttcctgctc agtaaggagc ctcggggaac cgctgtctcg tttctcttcc ttcacgcgca
     3361 cacctccact ggttcaaggg ggagttctct cctcctcccc ttggacccgg aaagttatgt
     3421 acgtatcgtc cggggctttt ttcggaagaa attatctcca cgaacgcaga ttcttcctgc
     3481 atgcccctaa gggagctgga agaagccctg gatgcgtttg cgaacgcagg tgaaccattc
     3541 ctcctgtcag aagtactcgc ccgggcaggc aagaaggggc atatcctcca cacagcggaa
     3601 gcactctggc caatgttcac caccctcgac ctcgtcgcgg agcgggtgcc gggagacctt
     3661 cgcctggcaa agagaccctc ctcaccccag aaaaccctca ccctggatga acgccgcagg
     3721 ctggacgcat tcttcaggaa accggaactt ccacccggaa tagaagcctg catccaggag
     3781 tatgtccatg caaagacggg taaagcctgg aacgaccctt ctgtcctcca gaagatccgg
     3841 cgcgcgatcg tgtcccagaa ggacgaatac tggcgcgagg gagaaaaaaa gagggtaatg
     3901 tatcagaaag ggtacgatgt actcgggtac ctggcctacc aggcgccggt atactttatc
     3961 caggccatgc acctcctgta cgacctttca ctggaaggtt gcctgaagca acacatgaga
     4021 gtgcttgacc ttggttgtgg ccccggagtg gtcccgcttg cgatcagcag ttttctagaa
     4081 aggatcggcg gatgttctgc cgagatctac tcggtcgaga agtcggagga gttcatcgag
     4141 gcttttcgtt tcctggccga ccacgtcccc gccccaaggg ggtcagtcac tctccatccc
     4201 cctctcaacg ccgacctggt cacggtccct ctctcatgcc ttcctccctc gcttgacctg
     4261 attgtcctcc agaatgtctt aaacgagatg tcagggcact cgccacgcga gaagggcaga
     4321 cttgtatgtc ggttcgcaga agccctctct cctgaaggga gcattctcct cgtggagcct
     4381 gcagatatgg tcaactcgat ggatctccgg gtcgtggtcc accatgcaac cggatccgga
     4441 ctcttcctcc atgcgccgtg caggttcctc tggaacaccc agtgcacacc agaccggtgc
     4501 tggagtttcg tggagaagcc gccaatccac gcgacaggac tcatggaagc gctcgcatcg
     4561 ggggaagagg ggtacaggta ctgtaacacc gatatcaagt actcgttcgc cctgctccgc
     4621 aaggttcccc caaaccctgt cccgggcctc cctctctcgc cgcgtaacta tgcgcggctc
     4681 tctgccctgc accggcacat caaaaggagg atcaacgttg caggcgtggt gatgtccggc
     4741 aacctcggcg accacaagac ctacgtcatc aagatatgcg atgggacgac gaaaaaaccg
     4801 gtctatgcaa tcctgccatc ctaccatctc tctcacgaaa acagcgcgct cctgtcgatt
     4861 ccctacggct ccattgctga atttacatcc gtccttgcca ggttcaacga gaaacaccat
     4921 gcatataacc tccttgtgag cagggagagc agagtaaagg agtttcccgc acaaagtaga
     4981 gccgcagact ggaatctcac aggtccccgt gaaaaaagga ataaactgaa aaaatgatcc
     5041 cttctcagta tggctctcca tgcaagggcc gcttgaatag ggattccagc cgggcacgcc
     5101 gatcacccgg gcactgtcat ggcccgttct tctgcggaac tccttttcct tatcgtgaat
     5161 agaagagtga ctttttcacc ctgaagtcct tcacccgatg cttcatggaa aatttccatt
     5221 tcttcgtccc tttgacatgt atttatggct tggcccgccc atacaacatt gacaggtgca
     5281 cgacaggcag aatcgatcgc aatcccgaca gaatctgcca gagagagagg gcaaaaacga
     5341 tgcggcacat catatccatc cgtgatctgg acagggccga gatcaatgga atactggacc
     5401 atgcacaggc cctccaggaa caggattacg atccccaggc cctgacaggg aagcttcttg
     5461 gtctcctctt ctttgaaccc agcacccgga caaggatgtc gtttgcgtct gccgtggccc
     5521 gcctgggcgg gcattcgatc tcgatggaca gcgtggaggc aagctccatg gtaaagggcg
     5581 agacccttgc agacaccata cgggtggtga gcgggtacgt cgatgccatc gtccttcgcc
     5641 atcccaagga aggtgcagcc cgcctggcaa gtgagttcgc gcaggtcccc gtcatcaatg
     5701 ctggcgacgg ggccggccag catccttcgc agacgctcct tgacctctac accatacggc
     5761 agtcgatgcc actcgagggt gtcaccgtcg gcctgcttgg ggatctccgg tacgggagga
     5821 ccgcgcactc gctggcgcat gccctctccc tttaccgggc cacgctccac accatatccc
     5881 caaaggggct tgaactccct tcgggccttg cgggcgagct ccgtgactca gggaccgaag
     5941 tgatcgagca tgacgatgtg caggaggtgg tgaaggatct cgatgtcctc tatgtcacca
     6001 ggatacagcg ggaacggttc ccggactcat cctcttattt caaggtcgcg tcaagctacc
     6061 gggtgacccc ggaactcctg gaaggagtac gggatcacct cgtagtcctg catccacttc
     6121 ccagggtcaa cgagattgat cccagggtgg atcacctgcc ttacgcccgg tacttcgagc
     6181 agtcggcaaa cggcatacct gtccggatgg ctatgcttct gcgggtgatg gaatgaagaa
     6241 aggaaagaac gcaccgaaag gccttctcat cagtcccatc gagaacggca cggtgatcga
     6301 ccatatcacg gcgggagaag ccttgaatgt cctccgtata atcgggatta ccgggaccac
     6361 ccaggagtcc atatcagttg caaccaacgt ggagagcggc aagatcgggt tcaaggatat
     6421 cgtgaagatc aagaacaggg agcttcgaaa ggaagaggtg gacaagatcg ccctgatcgc
     6481 cccgcgggct tccatcaaca tcatccgcaa ttacaaggtc tgggagaaga agggggtcga
     6541 gatcccctca gaggtgatcg ggatcgtaag gtgcccgaac cccgggtgca tatccaacac
     6601 gaacgagccc attgagtcct gtttcctcgt gcagcccaag ggtctgcgat gcgtctattg
     6661 cgactggctt atctcgaagg atatcacgag ccatatcatc tgattatctc acggcttttt
     6721 tttcacttga tagccacggg aagtgagcac tcctcctccc
//
LOCUS       DAOQYR010000104         6700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_11792_length_6700_cov_7.783898, whole genome shotgun sequence.
ACCESSION   DAOQYR010000104 DAOQYR010000000
VERSION     DAOQYR010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6700
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11792_length_6700_cov_7.783898"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PKM87_10175"
     CDS             complement(<1..726)
                     /locus_tag="PKM87_10175"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNQ30473.1"
                     /translation="MERWLLFLLAMAPGFLWLYYFYSKDRYDPEPVVWVLGIFLLGAT
                     VTVPVAVLEGVLGAVAGGILAAVVVAPVCEEIAKFFVVKKTVYRSRVFDEPVDGIIYA
                     AAAGRNGGGSGSFAGDVFLPWEGGSSYYSKWSNGPSSDPDYFPIAVWLQSPHNADAYR
                     AIGINTFVGLWEGQTEDQLGRLAGSGMPVLCSRSATGLGSSSNSVIRAWTHVDEPDNA
                     QADGQGGYGPPISTETVLAGYQSM"
     gene            complement(767..1828)
                     /gene="corA"
                     /locus_tag="PKM87_10180"
     CDS             complement(767..1828)
                     /gene="corA"
                     /locus_tag="PKM87_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNQ30474.1"
                     /translation="MPRRRRVMPIKPGLSPGTLAIDGKHTEGPVRITVIDYDAAHYTE
                     RQVASVEDCYPFRDTETVTWINIDGLGNTAVIEKLGKHYQIHPLILEDILNTGQRPKM
                     EDLEGYLYVNLKMLQLAGQEKTIKGEHVSMIIGSNYLLSFQEDAGDVFDPVRERIRKE
                     GRIRRFGPDYLAYALIDDIVDNYFLVIEHLEEEAEDLEEELVQNATPASLQKINRLKK
                     EMIFLRKSVWPLREVITAIERSESPIIRDTTIIYVRDVYDHVIQVIDTLETIRDMVSG
                     MIDIYLSGLSYRMNEIMKVLTLIATIFIPLTFVAGVYGMNFRYMPELAWEYGYVMVWG
                     VMIAMVVGMLLYFRKKHWV"
     gene            2189..3319
                     /locus_tag="PKM87_10185"
     CDS             2189..3319
                     /locus_tag="PKM87_10185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNQ30475.1"
                     /translation="MKILHISKKYPDAIGGDAQVVRSLEIQQEKAGHEILILTANTRE
                     LRKKRNVRIFGIFDTAANLDRITPKRMLSLVCFLFASIRAIAAEKPDVIHTHSAELGF
                     LVSFPASVLGIPVIQTCHGICFNDTGFSPLKRAVEAFLLKYGIFECITTVDGSSIPAF
                     SRLDISHVKYVPNGVDSALFKVKRDAQARPLMQFLCVGRLEHQKGLDILIEAASVLRK
                     ATNDFCITIIGDGSLRDELARQISAGSLDVHVQLLGSVPRDELVRQYACSDAFVLPSR
                     WEGMPLTLLEAWAAGLPVIVTDVGGITQICRDRENALVIQPGKPSQLCRAMRLLMDHP
                     DLGKRLGSEGNHIARERYSWDRIVDTYHFLYREAIQHNSRRK"
     gene            3501..4463
                     /locus_tag="PKM87_10190"
     CDS             3501..4463
                     /locus_tag="PKM87_10190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ30476.1"
                     /translation="MISIVIPNYNGKHFLEGCLSSIAAQTYHDFETILVDNASRDGSA
                     SFVRERFPWVLIVENPLNLGFAGGTNSGIRQAKGQYILTLNNDTRLEPDFLEQVKIAI
                     ESESMVGVVAPKMLLMDGRINSTGMCISRSGAAWNRGMFENDCGQYNAPEPVVGACAG
                     AALYRREVFDDIGLFDEDFFLYHEDVDLSFRAFLAGWNCLYWPSAVVWHENSGTLDYG
                     SDLCIYYVNRNILWFVAKSYPSWLLLSSLPWIIGRNIASVAFYALNGHIRVIVKSKMD
                     ALRGLPAAAGKRSSVKICVSRNKIMKYVRTWWQKESMRQNGAVK"
     gene            complement(4672..4809)
                     /locus_tag="PKM87_10195"
     CDS             complement(4672..4809)
                     /locus_tag="PKM87_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30477.1"
                     /translation="MANPLYSPYLAKNAFSSIVQSLSHQAFQEPTGKYGTGFALVGGM
                     V"
     gene            4808..5767
                     /locus_tag="PKM87_10200"
     CDS             4808..5767
                     /locus_tag="PKM87_10200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ30478.1"
                     /translation="MTAAGPEVSVIIVNYNGERFLKECLDSLRAQIYTDFEVILVDNH
                     SSDRSIEIAGHTYPEITLIRNSTNDGFARGTNDGIRASHGQFILTLNNDTRLDPGFID
                     EIVRPHLRDGKVGICASKMLYPDGRINSTGTCISRSGAAWDRGMSEPDHGQYNQPGEV
                     FGACAGAALYRKEMLDEIGLFDEDFFMYLEDVDLSFRARHAGWRCMFVPDAVAYHHHG
                     GTSGVGSDTAVYYGNRNIVWVAFKNFPRKLLLSSLPWIIGRTIGVIVYYALKGQLRVI
                     LRSKIDALYGLNLSLKKRKTTIHKVPDGEVSSWVHTWYSPGRQ"
     gene            5778..6530
                     /locus_tag="PKM87_10205"
     CDS             5778..6530
                     /locus_tag="PKM87_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ30479.1"
                     /translation="MQYRDLIWNLTITDLKVKYQSSVLGFAWSLLNPLLMMLVLYFVF
                     ANVFRFNQEHFALYILVGIIAWRFLANGTSTAMLSIVAKPSLVTKIYIPRHILVLSST
                     LSTFISSILEFCVLIPLLFIFGANITPYILFFPIAHAIFFFIVYGLSLVLAALYVYYR
                     DLNQIWEVVIQLGFFLAPICYPITIVPPQFLPYYVLNPVTILIETYRNFLIYGLPPGL
                     RSMAYLVISATLLMVIGKFVFNRLERRFAEVI"
BASE COUNT         1525 a         1816 c         1761 g         1598 t
ORIGIN      
        1 catcgactgg tatccggcaa ggaccgtttc cgtggatatc ggcggcccgt acccgccctg
       61 gccgtccgcc tgggcgttgt caggctcgtc aacatgggtc catgccctga tcacggaatt
      121 gcttgagctg ccgaggcccg tggcgctccg ggagcagagg accggcatcc cgctcccggc
      181 cagccggcca agctggtctt ccgtctgccc ttcccagaga ccgacgaagg tattgatgcc
      241 gatggcccgg taggcgtccg cgttgtgagg cgactgcagc cagaccgcga tgggaaaata
      301 atccgggtcg gacgatggac cgttggacca ctttgaataa taggaagacc cgccttccca
      361 gggcagaaag acatcaccgg caaatgatcc ggaaccgcct ccgttccgtc cggccgccgc
      421 ggcatagatg atcccgtcaa ctggctcgtc gaacacccgg ctccgataga ccgtcttttt
      481 gacaacgaaa aattttgcaa tctcctcgca taccggggcg acaacgactg cagcaagtat
      541 tccgcctgca accgccccga gaacaccttc caggaccgca acagggaccg tgacagtggc
      601 tcccaggaga aatatcccca ggacccagac taccggttcc gggtcatacc tgtcctttga
      661 gtagaagtag tagagccaga ggaaccccgg cgccatcgcc agcaggaaga gaagccaccg
      721 ttccatcccg ctcatcctgc tgcagtatcg attgccatgg cccgcatcag acccagtgtt
      781 tcttcctgaa atagaggagc atccccacca ccatcgcgat catgactccc cagaccatta
      841 cgtacccata ctcccatgcg agttctggca tatacctgaa gttcatgccg tacactcccg
      901 caacgaaggt aaggggaatg aatatggtgg caatcagggt gagcaccttc atgatctcgt
      961 tcatcctgta cgagaggcct gagagataga tgtcgatcat acccgagacc atgtcgcgga
     1021 tggtctcgag ggtgtcgatg acctggatga cgtggtcgta gacgtccctg acgtagatga
     1081 tggtagtgtc cctgatgatc ggagattccg acctctcgat ggcggtgatg acctccctga
     1141 gcggccagac tgacttccgg agaaagatca tctccttttt tagccggttg atcttctgaa
     1201 gagacgcggg ggtcgcgttc tgcacgagct cctcctcgag gtcctctgcc tcctcctcga
     1261 ggtgctcgat gacaaggaag taattgtcca cgatatcgtc tatcagggca tatgccaggt
     1321 aatccggccc gaacctccgg attctcccct ctttcctgat acgctccctc accgggtcaa
     1381 agacatcccc ggcgtcttcc tggaacgaga ggaggtagtt tgacccgatg atcatgctga
     1441 catgctcacc tttgatcgtt ttttcctgtc cagcaagctg cagcatcttg aggttcacgt
     1501 agagatagcc ttcaaggtcc tccatcttgg ggcgctggcc cgtgttcagg atgtcctcga
     1561 ggatgagggg gtggatctgg tagtgtttcc cgagtttctc gatgactgcg gtgttcccga
     1621 ggccatcgat gttgatccag gtgactgtct ccgtgtcacg gaacgggtaa caatcctcga
     1681 cgctggcgac ctgtcgctcg gtataatgcg ccgcatcgta gtcgatgact gtgatcctta
     1741 ccgggccctc ggtgtgcttt ccatctattg cgagcgttcc cggggagagt cccggcttga
     1801 ttggcataac acggcggcgg cggggcatgg gagatttcct gtttactatg aaaatggctg
     1861 cgccattttc ataaatgcgt atgcttgtgt ctcaaagaga atcatgtagt tttttctggg
     1921 ggatgatcgg gagtatttct cctgtattgg ttaaagatat ctcctttcga aggggtgaat
     1981 aggttcggat tctatccaca gacatgaaga tatgccgcgt ttgccaaccc cacaatccca
     2041 aaagagtaac ctcctttatt aattgcaatc agagagagga tatagtgatg aggaaaaaag
     2101 atacctggga cgatccttca cgacgaccca ccggattgcc gcccggcatc ccggaccaga
     2161 tctgatatag cgcccggaaa ttgccgatat gaaaatctta cacatctcca agaagtatcc
     2221 tgacgccatc gggggggatg cacaggtcgt ccgcagcctc gagatccaac aggagaaagc
     2281 aggccatgaa attcttatcc ttactgcgaa tacaagagag ttgaggaaaa agaggaatgt
     2341 gcggatcttc ggaatctttg atactgcagc gaaccttgac cggattaccc caaagaggat
     2401 gctgtcgctg gtctgctttc tcttcgcctc cattcgggcc attgcagccg aaaaaccgga
     2461 tgtcatccat actcactcgg ctgaactggg cttcctcgtc tcatttcctg ccagtgtgtt
     2521 gggcataccc gtaatccaga catgccacgg catctgcttc aacgacactg gtttctcacc
     2581 cctcaagcgc gccgtcgaag ccttccttct gaaatacggg atatttgagt gtatcaccac
     2641 ggtcgacggg agcagtatcc ccgctttttc taggctcgac atctcccatg tgaagtatgt
     2701 tcccaatggg gtggacagtg cactctttaa ggtcaagaga gacgcacagg caaggcccct
     2761 tatgcagttc ctgtgcgtgg ggaggcttga acatcagaag ggactggata tactcatcga
     2821 ggctgcatct gttttaagga aagcaacaaa tgatttttgc atcactatta tcggcgatgg
     2881 atcgctaagg gatgagcttg cacggcagat ttctgcagga tcgctcgacg ttcacgtgca
     2941 acttctcgga agcgtgccaa gagacgagtt ggtgcggcag tacgcatgtt ctgatgcatt
     3001 cgtactccca tcgcgctggg aggggatgcc tttgacactc cttgaagcct gggcagcggg
     3061 actccctgtc attgtcacag acgttggtgg gatcactcag atctgtaggg acagggagaa
     3121 tgccctcgtg atacaacccg gaaaaccctc gcaactctgc cgggcgatgc ggctcctgat
     3181 ggaccatccc gatctcggga agaggcttgg atcagaggga aaccatatcg caagagagcg
     3241 gtattcatgg gaccgtatcg ttgataccta ccattttctt tatcgggaag cgattcagca
     3301 caacagccga cgaaaatgaa gccgttatca atgatccgtt tccgatgctt gcgcccacca
     3361 ccaggagatt ccggtgtgga caaaggggag gagattaccc gttttatttc gcttcttgtc
     3421 gcgaaagcta ttgtaccttg tgacccccat aatgagatac gaacgcagga gtaatcggtg
     3481 agggagggaa tacacatccg gtgatcagca ttgtcatccc caattataac gggaagcatt
     3541 tcctcgaggg gtgcctctcc tccatcgccg cgcagacata tcacgatttc gagacgatcc
     3601 tcgtcgacaa cgcatcccgc gacgggagcg catcctttgt aagagagcga ttcccctggg
     3661 ttttaatcgt tgaaaatcct ctgaatctcg ggtttgcggg cgggacaaat tcagggatac
     3721 ggcaggcgaa agggcagtat attctcactc tcaataacga tacacggctt gagccggact
     3781 tcctcgaaca ggtgaagatc gcgatcgagt ccgagtccat ggtcggggtc gtcgcaccca
     3841 aaatgcttct catggacggc cgcatcaatt ccacgggcat gtgcatttcg cggagcggtg
     3901 cggcctggaa ccggggaatg ttcgagaatg actgcggcca gtacaatgct ccagaaccag
     3961 tggtcggcgc atgtgcgggc gcggcactct accgccggga agtgttcgac gatatcgggc
     4021 tctttgacga ggatttcttt ttataccacg aggacgtcga cctctccttc cgggccttcc
     4081 ttgcaggatg gaactgtctt tactggccga gcgctgttgt ctggcacgag aacagcggga
     4141 cgcttgatta cggctccgac ctctgcatct actacgttaa caggaacatc ctctggtttg
     4201 tagcaaagtc gtatccgtcg tggctgctcc tctcctccct gccctggata atcgggagaa
     4261 atattgcatc ggttgcattt tacgcgctga atggccatat ccgcgtcatt gtaaagtcaa
     4321 agatggacgc actgcgcgga ctgcccgctg cggcaggaaa gaggtcttcg gtgaaaatat
     4381 gcgtttccag gaacaagatc atgaagtatg tccggacctg gtggcagaag gagagcatga
     4441 gacaaaacgg ggccgtgaaa tgatgaacgc aagaagaggt aatccgtatc gttgttcttt
     4501 cttcccagga tttcatttag atcgttttcc ccaggacatg aaagccccat cctgggagga
     4561 acgtgtgaga gaagtttgcg ttcatccggt atccaacttc cacatcatag agggacaatg
     4621 gcataaccag aaaatcccaa tagagttcga caccgtctct ttgaagcctt ctcacaccat
     4681 tccgccaaca agggcaaatc cggttccata tttaccagtg ggctcctgga atgcctggtg
     4741 ggagagtgat tggacgattg acgagaatgc gttttttgcg agataaggag aatacaaggg
     4801 gtttgccatg acagccgcgg gtccagaagt aagcgtgatt attgtcaatt acaacgggga
     4861 gcgtttttta aaagaatgcc tggactcgct tcgtgcacag atttacacgg atttcgaggt
     4921 gattcttgtc gacaatcatt cgtctgaccg tagtattgag atagccgggc atacatatcc
     4981 cgaaatcacc ctgattcgga attccaccaa tgatggattc gcccgaggta caaatgacgg
     5041 gatacgggcg tcacacggtc aattcatcct taccctgaac aacgacacgc ggcttgaccc
     5101 gggtttcatc gacgagattg tgcgccccca tctccgtgat gggaaggtag gcatctgcgc
     5161 ctcaaagatg ctctatcccg acggaagaat caactcaacc ggcacatgca tctcccggag
     5221 cggtgctgcg tgggacaggg gaatgtccga acctgaccat ggccagtaca atcaaccggg
     5281 ggaggtattt ggggcgtgtg caggcgcagc actctacagg aaggagatgc tcgatgagat
     5341 cgggctcttt gacgaggact tcttcatgta tttggaggat gtggaccttt cattccgtgc
     5401 gcggcatgcg gggtggagat gcatgttcgt tccggatgct gtcgcctatc atcaccacgg
     5461 aggaacttct ggagtgggat cggacactgc cgtatattac gggaatcgaa atatcgtctg
     5521 ggtggcattc aagaactttc cccggaaact cctgctgtcg tccctcccct ggatcatcgg
     5581 gaggacgatc ggtgtgattg tatactatgc cctgaagggt caattgcgag tgatcctccg
     5641 gtcgaaaatt gatgcactct atggactcaa tttatcactt aaaaagagaa aaactactat
     5701 tcacaaagta ccagacggtg aggtctcctc gtgggtccat acctggtatt cccctgggcg
     5761 gcaataacat gtggctgatg caatatcgcg acctcatctg gaacctgacc atcaccgatc
     5821 tcaaggtcaa gtaccagagt tcggtcctcg gattcgcatg gtcgctcctc aaccccctcc
     5881 tgatgatgct cgtcctttac ttcgtatttg ccaatgtctt tcgcttcaac caggaacact
     5941 tcgccctcta cattcttgtc gggatcatcg cgtggcggtt cctggcaaac gggacttcca
     6001 cggcgatgtt gtcgatcgtg gcaaagccga gcctcgtcac gaagatctac atcccccgcc
     6061 atatcctcgt gctctcatcc acactttcca ctttcatcag ctcaatcctc gagttctgcg
     6121 tccttatccc attactcttc atcttcggcg caaatattac accctatatt ttatttttcc
     6181 caatcgctca cgcgatcttc ttcttcatcg tctacggcct ctccctcgtg cttgcggcat
     6241 tgtatgtgta ttaccgggac ctgaaccaga tctgggaggt cgttatccag ctcgggttct
     6301 tccttgcacc gatctgttac ccgatcacaa tcgttccacc tcagttcctt ccctattacg
     6361 tcctgaaccc tgtcactatc ctgattgaga cctatcgcaa tttccttatc tacggccttc
     6421 cgcccggact gcggagcatg gcctacctcg tgatttcggc aactctcttg atggtcattg
     6481 gtaaattcgt attcaaccgc ctcgaacgaa gatttgcgga ggtgatctga atggccggaa
     6541 atcagtattc gaggaacaaa aagaatcccg atcacgacga tgccattgtg gttgaaaacc
     6601 tctcgaagca gttccgcatc ccccacgaga gaaagatcac ggtataccag cacctcgcag
     6661 gactgattgc ggggagctat tcatacgaga ttttcgatgc
//
LOCUS       DAOQYR010000105         6648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_11901_length_6648_cov_7.974822, whole genome shotgun sequence.
ACCESSION   DAOQYR010000105 DAOQYR010000000
VERSION     DAOQYR010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11901_length_6648_cov_7.974822"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..3730)
                     /locus_tag="PKM87_10210"
     CDS             complement(128..3730)
                     /locus_tag="PKM87_10210"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3344 domain-containing protein"
                     /protein_id="HNQ30480.1"
                     /translation="MQKKHSILFITLALVVAVSWIMPAGADPYLGGIPLETVRDGQVS
                     GGLFVDAGFPMSTDASASFTLPAHTAIQWARLYVVVYCGHMQNNYQAQAEVSFNGGRG
                     SSTLATEILNVPYTFPGEGGTGPVRLNDHTNRVTSDYLMWYDVKSHIQDRNVQARIKT
                     TRPAGYSGTFDGRVKAIVLVVAYDDGDQDRVVYWINHGHDEDSELTEEILGETYAGET
                     SFSTSSLDEEWEQADLTVVHLASENADYEFNRDSLAAGTPQGTYAGLERHDVTDSIWR
                     GKDSDLFYDNTGRYYKIMLATLSVRYPGSDAGSLKVTSAPAGATIFIDGEEQEAATNT
                     TLPEIPQGYHIVTVEKEGYRDPGPQQVTVQTGVTSTVHFTLQEARGTLSVASDPAGAE
                     VLIDGVKHTALTPCMIPDVPAGERDVTIRLSGYQDWKETVEVVDGETVSIDEELVPGG
                     RAGSSSGGDSTVTSTGLSGYVGSALTLYQQGTLNGTFFSTVAGDYTGMIPSGKQHVYT
                     LPDAVPAEGEIALSRLYLYTTWSHDMTKREGLPAMPAVSWNGKLLKADATYTDRKGDG
                     IYDYPVQTFAYDLKEVIHPGDQGKVTVVNSGGSGREFAAYGVMLIALVKDHAAPEITY
                     WICEGSDVVYADPRFGTTAEDARTSAEFAGNVEKEGIGEATLYLVSTAASGEGGDQHQ
                     VFFNDGEWYNPLTEGSSGVSVAALDVLPWLERSKNRLEVASLPVSGAGDYMENRNAVL
                     VLTRGTPTVAGDSVAPVPEATRAPESTRPAVSAPGGIVQETSADPGEILPSSNGHLIP
                     PPKDAGFPGSIVEWFFARVFEIFGIHRSPSHEVTPTGAPVTGDFREGEGEIHGANVGS
                     PAFPVPGPVIVSEEGRTENPPWMEEELTEEPFLTAEEDAAPASPKGSQEKAVTGGIFV
                     DSYPRGADIFIDGRKVPAKTPFVANWLKGGMHSIRLEMESFTFTPESRQVWVTPGEIA
                     TAEFSSGATMVRRLAINSSGWSGAQFTINGKGPILRIPSEVNVEGISSFVTVFSNGAW
                     HSKTILARIVDGSEVIIENAAEGLAGIELSSTPSGAMILIDGFNTGKVTPALIENLSP
                     GQHRIDLLLPGYYPSSQVVTLVDYRNEQVDMRITVPLDAYYSGSLTIESDPPGAKISF
                     FSKDTGRKTPATFDCLAIGTVSGRVRWDDAERDFEVDILPGENLTVNLSPPEKK"
     gene            complement(3735..4955)
                     /locus_tag="PKM87_10215"
     CDS             complement(3735..4955)
                     /locus_tag="PKM87_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ30481.1"
                     /translation="MNSRLVEFFQFAVRQLFRRKFRVILTVAGIAIGVAALVGTVALG
                     EGIRTQAVDAIRTQSDLTLIEALPGVEGNVLQLVTPTRAAAAGSLSGVAASTPVVRGT
                     FATRGQTYVQVVGIPADGLDGVLHPTYLRGSGFAAGSNEVVFGAALGEKLQRYEGVRI
                     GDPFVLIRRSYDAQGRPVDEETTVVPAGVMEERGDAYDQVLLMDLDLAVRLGEGDGGY
                     SGILIRAEGPDRVFSVAEGVRSLGLSPRGSFEQIEAVNRMMDMVVIFLGIFAGLSLLV
                     GGLMIASTMITSVYERTREIGIAMAVGASEGDVMEMILAECAVLGVIGGVCGDLLGVS
                     FAAVLNMLGGPFLASRFGDAFAGLLDTEIALVTWPLLAAGLLLAVGLSLLAGLYPAWK
                     ASRLNPVEAIRSGR"
     gene            complement(4952..5620)
                     /locus_tag="PKM87_10220"
     CDS             complement(4952..5620)
                     /locus_tag="PKM87_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ30482.1"
                     /translation="MTSPILETRALSRHYPGGVRALEGVDLRIEKGEFVAIVGRSGSG
                     KSTLLNIVGGLDAPTGGSVVINGTPLDFSNRKALISFRRGMVGFVFQQFNLIPSLTAR
                     ENVEYPLLFNYRPPAERQEKAGILLSMVGLDGRVSHYPGQLSGGEQQRVAIARALVAD
                     SPLILADEPTGNLDSATSDGIFSLLQRLNRERGVTLVVVTHERELASYSDRTVEMRDG
                     KVVA"
     gene            complement(5617..6600)
                     /locus_tag="PKM87_10225"
     CDS             complement(5617..6600)
                     /locus_tag="PKM87_10225"
                     /inference="COORDINATES: protein motif:HMM:NF023252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3344 domain-containing protein"
                     /protein_id="HNQ30483.1"
                     /translation="MTSRNVLPGPLIAAFLIVCAAPVAASYAGDLPLQLVFSDSLQGT
                     TSFTLGDGGYSGTLSPGAGYVASFARELPPGAEVRFERLYIYWTWSRIDQAASYPTME
                     ARHGGPGGPVLGRAARYADSKGFASKNDYFSGMDSYLLPGQAGDRVTVLVNNTAEDGS
                     TFIVQGTALLTVYEDPGAPESAIWIAEGADLLYRSYGIPPEIATTRVEFPGRIDLPQV
                     KSARLLLVAPSAGFAREEIPEMNQILLNTPGSGSLPPLVEPVLRVLFPHYQGKTWTDV
                     FTADETRQIGIASREIRPFLRYSDNFIEVRDNGDYFQVCNAVLRVEYLELA"
BASE COUNT         1215 a         2145 c         1989 g         1299 t
ORIGIN      
        1 ccatcaccgt cccctcagcg gggagccggg ccaccggtcc cccggaaggc ggcgtgaggt
       61 gtgaattccc ttctacttct gtcccttcca aaaggtgggg gaggatgctc ctgaacaggt
      121 gatcgtatca cttcttctca ggcggcgaga gattgacagt gagattctca cctggcagga
      181 tatccacctc gaagtcccgc tcggcatcgt cccagcggac ccttccactg accgtcccga
      241 tcgcgaggca gtcgaatgtt gcaggggttt ttctcccggt atccttgcta aagaaggaga
      301 tcttcgcacc gggaggatcg ctctcgatgg tgagggaccc tgaataatat gcgtccaggg
      361 gaaccgtgat tcgcatatcg acctgctcat tccggtagtc gacaagggtt accacctgcg
      421 aggaggggta ataaccgggc aggaggaggt cgatgcggtg ctgtcccggg gagaggtttt
      481 cgatgagagc aggtgtcact ttcccggtgt tgaatccatc gatgaggatc atggctccgg
      541 agggtgtcga gctcaactcg attcccgcaa gtccttcggc cgcattctca ataatgacct
      601 ctgaaccatc cacgatccgt gcaaggatgg tcttcgaatg ccatgcacca ttggagaaca
      661 ccgtgacgaa tgaggagatg ccctcgacat tcacctctga aggaattcgg aggatcggtc
      721 ccttcccatt gatggtgaac tgggcgccgg accacccgct cgagttaatg gcaaggcgcc
      781 gcaccattgt ggcaccactc gagaactcgg cagtcgcgat ctcgccgggt gtgacccaga
      841 cctgcctgct ctcgggagtg aaggtgaatg actccatctc gagcctgatg ctgtgcatcc
      901 cccctttcag ccagttggcc acaaaaggtg tctttgcagg caccttcctc ccgtcaatga
      961 agatgtcggc cccccgcgga taggaatcaa caaagattcc gcccgtgaca gccttctcct
     1021 gtgagccctt cggggaggcc ggtgctgcat cttcctctgc agtgaggaag ggctcttcgg
     1081 tgagttcctc ttccatccag ggagggttct ctgtcctgcc ctcttcactg acgatgaccg
     1141 gcccgggcac agggaacgcg ggactgccga cattcgcccc gtgaatctcc ccttctccct
     1201 ccctaaaatc tcccgttaca ggtgctcccg tcggcgttac ctcgtgggaa ggagacctgt
     1261 gaatcccaaa gatctcgaat accctggcaa agaaccattc cacgatactc ccgggaaacc
     1321 cggcatcctt tggcgggggg atgaggtgcc cgttggacga tggcaggatt tctcccgggt
     1381 cagccgacgt ttcttgcact attcctcccg gggcggagac tgctggccgg gtgctctccg
     1441 gtgcacgggt ggcttcaggt accggagcaa cggaatcacc ggccactgtt ggtgttcccc
     1501 gggtcagcac cagcacggcg tttcggttct ccatgtagtc ccccgctccc gataccggga
     1561 ggctcgcgac ctcaagccgg tttttagacc gctcgagcca tggaaggaca tccagcgctg
     1621 cgacgctgac gccggacgat ccttccgtga gagggttgta ccattcgccg tcgttgaaaa
     1681 acacctggtg ctggtcacct ccctccccgg acgccgcagt gctgaccagg tagagtgtcg
     1741 cctcgcctat tccctccttc tccacgtttc cggcgaactc tgcactggtg cgggcatctt
     1801 cagcagtcgt gccaaagcgg gggtctgcgt agacgacatc tgatccctcg catatccagt
     1861 acgtgatctc gggcgcagcg tgatctttta ccagggcaat gagcatgacc ccgtacgcgg
     1921 caaattcccg gccactcccg ccgctgttca ccacggtcac cttgccctgg tcgccggggt
     1981 gaatcacttc cttcagatcg taggcaaagg tctggacagg gtagtcatag atcccgtcgc
     2041 ccttcctgtc agtgtaggtg gcatccgcct tcaggagttt tccgttccat gagaccgcgg
     2101 gcattgcggg caacccttct ctcttcgtca tgtcatggct ccaggtggtg tagaggtaaa
     2161 ggcgcgagag ggcaatctcc ccttctgcag gcaccgcatc cggcaaagta tagacatgct
     2221 gctttccgga tgggatcatc cccgtgtaat cgcctgcaac cgtcgagaag aacgtgccgt
     2281 tcagtgtccc ctgctgatac agcgtcaggg ccgaccccac gtaccctgat agccctgtac
     2341 tggtcaccgt ggaatcacca cctgacgatg accctgcacg gccccctggc accagttcct
     2401 cgtctatcga aaccgtctcc ccgtcaacga cctccactgt ctccttccag tcctggtaac
     2461 cagagagcct gattgtcaca tcacgctccc ctgcggggac gtccgggatc atgcaggggg
     2521 taagcgcagt gtgcttcacg ccgtcaatca ggacctcggc tcctgcgggg tcggacgcga
     2581 ccgagagggt ccccctggcc tcctggaggg tgaagtggac agtcgaggtc actcctgtct
     2641 gcacggtcac ctgctgcggg cccgggtccc tgtatccttc cttctccacc gtgacgatgt
     2701 ggtacccctg cgggatttcg gggagcgtgg tgttggtcgc cgcttcctgc tcctcgccgt
     2761 cgatgaagat cgttgctcct gcgggtgccg aggtgacctt caggctcccg gcgtcgctcc
     2821 ccgggtatcg gacggatagg gttgccagca tgatcttgta atagcgcccc gtgttgtcgt
     2881 agaagagatc tgagtccttt ccccgccata tgctgtcggt cacgtcatgc ctttcgaggc
     2941 ctgcatatgt accctggggc gttcccgccg caaggctgtc acggttgaac tcgtagtccg
     3001 cgttctcgct tgccaggtgg accaccgtga gatcggcctg ctcccactcc tcatcgagcg
     3061 atgaggtcga aaaagaggtc tcccccgcgt aggtctcccc aagtatctcc tcggtcagct
     3121 cgctgtcctc gtcgtggcca tggttgatcc agtagaccac ccggtcctgg tcgccgtcgt
     3181 catatgccac gaccaggact atcgccttga cccggccgtc gaaggtcccg gaataccccg
     3241 caggacgcgt ggtcttgatc ctggcctgta cgttccggtc ctggatgtgg cttttcacat
     3301 cgtaccacat caggtagtcg ctcgtgacgc ggttggtatg gtcattcaac ctgaccggcc
     3361 cggttcctcc ctcgcccggg aacgtgtacg ggacattcag gatctcggtg gcaagagtgg
     3421 aggatcccct ccccccgttg aatgagacct ccgcctgtgc ctggtagttg ttctgcatgt
     3481 gcccgcaata cacgacgaca tagaggcggg cccactggat cgcggtatga gcggggagcg
     3541 tgaacgaggc gctcgcgtcg gtcgacatcg ggaaccctgc gtccacgaag agtccgcccg
     3601 agacctggcc atccctgacg gtctcgaggg gtatcccgcc gaggtaggga tcggcgccgg
     3661 caggcatgat ccacgatacg gccacgacga gggcaagcgt gatgaacagg atgctgtgtt
     3721 tcttctgcat gattctatct ccctgacctt attgcctcca cagggttgag ccgcgatgcc
     3781 ttccaggcag ggtagagccc cgccaggagc gaaaggccca cggcgagcag gaggccggct
     3841 gcgagcagcg gccatgtcac gagcgcaatc tcggtgtcaa ggagaccggc aaatgcgtcc
     3901 ccgaagcgtg acgcgaggaa cggcccccca agcatgttca ggaccgcggc aaaggacacc
     3961 ccgagcaggt cgccacacac cccgccgatg actcccagca ccgcgcactc ggcaaggatc
     4021 atctccatca cgtccccctc cgaagccccg acagccatgg cgatcccaat ctcccgggtc
     4081 cgctcgtaga ccgaggtgat catagtgctt gcgatcatga ggcccccgac aaggagcgag
     4141 aggcctgcga agatgccgag gaatatgacc accatgtcca tcatgcggtt caccgcctcg
     4201 atctgctcga acgagccccg cggggagaga ccgagagacc ttaccccttc tgcgacggaa
     4261 aagacccgat ccggcccctc cgccctgatg aggatgccgc tataccctcc gtcaccctcc
     4321 ccgagccgga ccgcgaggtc aaggtccatc aggagcacct ggtcgtacgc gtctccccgc
     4381 tcctccatga ctccggcggg gacgaccgtg gtctcctcgt cgaccggcct tccctgggca
     4441 tcataggagc gcctgatgag gacaaaggga tcgccgatgc ggacaccctc gtaacgctgg
     4501 agcttctcgc caagagcggc cccgaacacc acctcgtttg atccggcagc gaagccggat
     4561 ccccggagat acgtcgggtg cagtacgcca tcgagcccat cagccgggat ccccaccacc
     4621 tggacatacg tctggcccct ggtggcaaac gtccccctca ccacaggggt tgatgccgcc
     4681 acacctgaca gactgcctgc ggcagccgcc ctcgtgggag tgacaagctg gaggacgttt
     4741 ccctccactc ccggcagcgc ctcgatcagc gtgaggtcgg actgtgtgcg tatggcgtcc
     4801 accgcctggg tgcggatacc ctcgccgagg gcgacagtcc ccacaagcgc agcaactcca
     4861 atcgcaatgc cggcaacagt gaggatgacg cgaaacttcc gccggaagag ctggcggacg
     4921 gcaaactgga agaattcgac cagccgggag ttcatgcgac caccttcccg tcacgcatct
     4981 ctaccgtgcg gtcggaatag gacgcaagct cccgctcgtg cgtgacgaca acgagcgtca
     5041 ctccccgttc gcggttcagc cgctgcaaaa gcgagaatat cccatcgctc gtggcagagt
     5101 cgaggtttcc ggtcggctcg tctgcgagga tgaggggaga gtcggccacg agcgcccggg
     5161 cgattgccac ccgctgctgt tcgcccccgg agagctgccc cgggtagtgg gaaacccgcc
     5221 cgtcgagccc taccatcgag aggagtatcc ctgccttctc ctgccgctcg gccggcggcc
     5281 tgtagttgaa gagaagcgga tattccacgt tctcccgggc tgtgagtgac gggatgaggt
     5341 tgaactgctg gaagacgaac ccgaccatcc ccctccggaa cgagataagg gctttccggt
     5401 tggagaagtc aagcggggtt ccgttgatca cgaccgaccc cccggtggga gcgtcaaggc
     5461 ccccgacgat gttgaggagg gtgctcttcc cgctcccgct cctcccgacg atggccacga
     5521 actccccctt ctcaatccga aggtcaactc cctcgagggc cctgactcct cctggatagt
     5581 ggcgggagag tgcccgggtc tcgagaatag ggctggtcat gccaactcca ggtactcgac
     5641 cctgagcacc gcgttgcaga cctggaagta gtcgccgttg tccctgacct cgatgaaatt
     5701 gtcggaatag cgaaggaaag gccggatctc gcgggaggcg atcccaatct gtcgggtctc
     5761 gtctgcggtg aatacatcgg tccaggtctt tccctggtaa tgggggaaga ggacccgcag
     5821 gaccggctca acgagcggcg ggagactgcc gcttccggga gtattcagga ggatctggtt
     5881 catctccggg atctcttccc gggcaaaccc cgcggaaggt gcgacgagaa ggagccgtgc
     5941 cgacttcacc tggggcaggt cgatcctccc cgggaactcc acgcgggtag tggcaatctc
     6001 gggcgggatg ccgtaactcc ggtagaggag gtctgcccct tcggctatcc agatggcaga
     6061 ttccggagcc ccggggtctt cgtacacggt gaggagagcg gtcccctgga cgatgaacgt
     6121 gctcccgtcc tctgcggtgt tgttgaccag cacggtgacc cggtcgccag cctgaccggg
     6181 gaggaggtag ctgtccatcc ccgagaagta gtcgttcttg ctcgcaaacc ctttgctgtc
     6241 ggcataccgg gccgcccgcc cgaggactgg cccaccgggg cccccgtgcc ttgcctccat
     6301 tgtcgggtag gacgcggcct ggtcgatcct gctccatgtc cagtatatgt agaggcgttc
     6361 gaaccggacc tctgcccccg gcggcagttc ccttgcgaat gaggccacat acccggcacc
     6421 ggggctgagg gtcccgctgt agccgccgtc gccaagggtg aacgaggtgg tcccttgcag
     6481 ggaatccgag aagaccagct ggagaggcag gtcgcctgcg taactcgccg cgaccggtgc
     6541 ggcgcataca atcaggaacg ctgcgatcag tggcccggga agcacgttac gggaagtcat
     6601 acctcgcgtc tgcccggttg tttcccttct cccgctcggg gatcacac
//
LOCUS       DAOQYR010000106         6642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_11914_length_6642_cov_8.409746, whole genome shotgun sequence.
ACCESSION   DAOQYR010000106 DAOQYR010000000
VERSION     DAOQYR010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11914_length_6642_cov_8.409746"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PKM87_10230"
     CDS             <1..475
                     /locus_tag="PKM87_10230"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNQ30484.1"
                     /translation="VDPVMVSTSRHALMEKDAWQDLVECLLPRATVVTPNIDEAEVLS
                     GIRPIQTLEEAREAGLRILDLGPRHVLVKGGHLPGDEAPDLLLAPGKEWVIATTRLPV
                     TVHGAGCCYSAALCAHLAMGCTARDAFSRSKDYMDRLIRASVRAPSGVHVLFPSP"
     gene            606..1640
                     /locus_tag="PKM87_10235"
     CDS             606..1640
                     /locus_tag="PKM87_10235"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNQ30485.1"
                     /translation="MDGIWRDILEAMPGPVCVVDAGKRIRYVNPSMVALRGGTAGDEI
                     VPGTPADEVLAPLPYEVQRSLYSVLRTGKVVTARISWIEREGGPLVLESTAFPVPGTG
                     GGMPELAAWTAAVIPGEVPAGEALAMEASRSKGKELLSVVRHDILNQLTILIGFLQFS
                     EDFIEDSRVREFLAKEEAAGHVIQALIEFTRDFQDLAVEDPRWMRLDAVIRSAQGRVD
                     TKGIEIVADAGTTEVYASPLIRHVFLTFIKNAVDHAHGLTRITLSQGVDGGDLVIACE
                     DDGPGIPESERKTLFDRGHGRNRGYGLWLAKEILSITGAKIREAGKEGGGARFEIRVP
                     AGMWREGDAS"
     gene            complement(1641..3308)
                     /locus_tag="PKM87_10240"
     CDS             complement(1641..3308)
                     /locus_tag="PKM87_10240"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNQ30486.1"
                     /translation="MITVLLVHDNSDALESTRAYLERMGEIRVDAVHSTKQALEMARR
                     RRYDIIVSYYRLPEVNGIEFLADMTGVELLKELKGTHAGIPFILYHRTDRDSLLIEDV
                     HQAAEVHGQREGSRGPVQEMRDILYQAVLRKKAERDQVLRCDMLSAILSVSPLWICQV
                     RGGNFEWVNSTMARSLGMDAGALAGQGVQAVFPGNEEYERAFRELCHRSDDQGWGHAD
                     TVLKRADGTLIPCRLSIRAMDPADPSRGQVIVCEDRSEHAKLIDAQKESDLRYREFLQ
                     NASSLIIKLDPEGVVTFFNRHAQAFFGYSEAEVLGKNIRDTLVPRKDGKASPGFVTDT
                     GANHEGGSLRIYEMQLRGGEPAWVAWHTRAIRNADGHLVEVVCIGHDITDHEHRDRRR
                     ISTAMWRDTVITGTDVKEEVFDSVLHICIEIAKEGREGKQVGTAFVIGDSDAVLVKSK
                     QLILNPFEGHPATDRMITNHDIKENIKELAQLDGAFVVRGDGRIEAAARYITIDTSKV
                     GIQKGLGTRHSSVAGITLVTRAIGIVLSQSGGKISIFKDGRMVQEIA"
     gene            complement(3436..4788)
                     /locus_tag="PKM87_10245"
     CDS             complement(3436..4788)
                     /locus_tag="PKM87_10245"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNQ30487.1"
                     /translation="MPGTLRNMYMKTISFVDLDLPEPLMRAVLDQGFEEPTPIQSLAI
                     PVIRERHDMVGQAHTGTGKTAAYGIPLLEQVDQKVPEVQAIVLCPTRELAIQVSEELR
                     KLARHLRGITILPVYGGQPIERQISVLKKGVQVVIATPGRLIDHLDRRTVSLSGIRLV
                     VLDEADEMLDMGFRDDIEIILKRTPTERQTVLFSATMSKEILALAQKYLKDPRMVKVV
                     HGRLAVPAIEQRYFEVRESDKVEVISRLLDFYNPKLSLVFCNTKRKVDEVSGQLQARG
                     YLAEALHGDMDQKERERVMSRFRTGASDILVATDVAARGIDVEEVGVVFNYDVPQDPE
                     YYIHRIGRTGRAGKSGKAFTFVSGKEMWKLRDIQKFAKIRIARHAVPADEDLRERRIV
                     NMIARIRENITAGDLEPFIARVEQVMGDEHTSLEVAAALFRMQSGGDAGVSRGKGRGR
                     "
     gene            complement(4916..5842)
                     /locus_tag="PKM87_10250"
     CDS             complement(4916..5842)
                     /locus_tag="PKM87_10250"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNQ30488.1"
                     /translation="MVSTEVRRVCEVMKGAEARAAEVRFITHAGRPFHKMGSYERRLQ
                     AYIVLLVSVVVIGVAGFSLLEGFSPLDSFYLTIVTIATVGYGDLHPATEAGKVLAVVV
                     IVAGVGCFVGLVANVVEHIAFRKERARRRQKTMMLVGIFFTQVGTSLLATCARGDLGR
                     QDLSRLLSGPKFADSPASAGITGALASRSYHLDCRELDLATLRDALGEKIDVLSVLLQ
                     NPDLDEHSKFTDLLQALFHLWQELSHREVLCGLPGADTAHLSGDLNRVYGLLVLEWVV
                     YLGHLKRHYPYLYSLALRTNPFDEKASVVIRE"
     gene            5841..6146
                     /locus_tag="PKM87_10255"
     CDS             5841..6146
                     /locus_tag="PKM87_10255"
                     /inference="COORDINATES: protein motif:HMM:NF022420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2769 domain-containing protein"
                     /protein_id="HNQ30489.1"
                     /translation="MTDTFEGVIRELKAKRKEERWKNYDTWKRSCCCPDCPSYNECAS
                     GGRELLYCILGMSIQCVREDRHCICKECPLYSTLGLSGKDFCMKGSEAAIRYERSVE"
     gene            complement(6247..6414)
                     /locus_tag="PKM87_10260"
     CDS             complement(6247..6414)
                     /locus_tag="PKM87_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNQ30490.1"
                     /translation="MIFKVVITPDSEDGGFIVSCPSLPGCHSQGETIEEALENIRDAI
                     SGCVAALNERA"
BASE COUNT         1187 a         2005 c         2145 g         1305 t
ORIGIN      
        1 cgtggacccg gtgatggtct ccaccagcag gcatgcgctg atggagaagg acgcctggca
       61 ggacctcgtc gagtgcctgc tgccgagggc aacggtggtg accccgaata ttgacgaggc
      121 agaagtgctc tccggtatcc ggcccattca gacgctcgag gaggcacggg aggcgggtct
      181 tcgcattctc gacctcgggc cacgtcatgt cctcgtgaag ggcgggcatc tcccgggaga
      241 cgaggcgccc gacctcctcc ttgcgccggg gaaggagtgg gtgatcgcga ccacgcgcct
      301 cccggtcacg gtgcacggtg cagggtgctg ctactcggct gccctctgcg cccacctggc
      361 aatggggtgc accgcaaggg atgcgttctc ccgctcgaag gactacatgg accggctgat
      421 ccgggcgtcg gtcagggcac cttcaggggt gcacgtcctc ttcccgtctc catgagcagg
      481 gcaggcaggg cttcttcccg caggggagtg gttccgctat gttcaaggca agacctgcac
      541 aagataatct tcacgggaga ggatatgtga acgaccgtgg caaaacaacc ggggggactg
      601 gtggcatgga tggtatctgg cgtgacatcc tcgaggcgat gcccgggccg gtctgcgtgg
      661 tggacgccgg aaaaaggata cgctacgtga acccctccat ggtcgccctg cgcggcggca
      721 ccgcgggaga tgagatagtc cccggcaccc ctgcagacga ggtgctcgcg ccgctcccct
      781 acgaggtcca gcggtcgctg tattcggtgc tgcggacggg aaaggtggtg actgcccgaa
      841 tctcgtggat tgagagggag ggcggccccc tggtcctcga gagcacggcg tttccggtcc
      901 ccggcaccgg gggagggatg cccgaacttg cggcgtggac tgccgcagtg attcctggcg
      961 aggtgccggc cggggaggcc ctcgcgatgg aggcctcgcg gagcaaggga aaggaactcc
     1021 tttcggtggt caggcacgat atcctcaacc agctgaccat cctgatcggc tttctccagt
     1081 tttccgagga cttcatcgag gactccaggg tcagggagtt cctcgcgaaa gaggaggccg
     1141 cgggccatgt catccaggcg ctgatcgagt tcacccgcga cttccaggac ctggcagtcg
     1201 aggacccgcg ctggatgcgg ctcgatgccg tcatccggtc cgcccagggg agggtcgata
     1261 cgaagggcat cgaaatcgtc gcggatgccg gcacaaccga ggtctatgcg agcccgctga
     1321 tccggcatgt ctttttgacg ttcatcaaga atgcggttga ccatgcgcac gggctcaccc
     1381 ggatcaccct ctcgcaggga gtggacggcg gcgacctcgt tattgcctgc gaggacgacg
     1441 gtcccggcat cccggagtca gagagaaaga cgctctttga ccggggccac ggcaggaaca
     1501 ggggatacgg cctctggctc gcaaaggaga tcctctcgat cacgggggca aagatccggg
     1561 aggccggaaa ggagggcggc ggggccaggt tcgagatacg ggttcccgcc gggatgtggc
     1621 gggaagggga cgcctcctga tcaggcgatc tcctgcacca tccgtccgtc cttgaagatc
     1681 gagatcttgc ccccgctctg cgagaggacg atgccgattg ccctcgtgac cagggtgatc
     1741 ccggcaacag acgagtgccg cgtcccaagg cctttctgga tccccacctt gctcgtgtcg
     1801 atcgtgatgt aacgggcggc agcttctatc cgcccgtcgc cccggaccac gaatgcaccg
     1861 tcgagctgcg ccagctcctt tatgttctcc ttgatatcgt ggttggtgat catccggtca
     1921 gtggcagggt ggccctcgaa cgggttgagg atcagctgct ttgacttgac cagcaccgcg
     1981 tccgagtccc cgatgacaaa cgcggtcccc acctgcttgc cctctctccc ctcctttgcg
     2041 atctcgatgc agatgtggag gaccgagtca aagacctctt ctttgacatc ggtcccggtg
     2101 atcacggtgt ccctccacat agccgtgctg atcctgcgcc ggtcccggtg ctcgtggtcg
     2161 gtgatgtcgt ggccgatgca gaccacctcc acgaggtgcc cgtccgcgtt gcggatggcc
     2221 ctcgtgtgcc aggcgaccca ggcgggctcc ccgccccgga gctgcatctc gtagatgcgg
     2281 aggcttccgc cctcgtggtt tgcgcctgtg tcggtgacaa agcccgggga ggccttccca
     2341 tccttccggg ggaccagggt gtcgcggatg ttctttccga ggacctctgc ctccgagtag
     2401 ccgaagaagg cctgggcgtg cctgttgaag aaggtgacga ccccttcggg gtcgagcttg
     2461 atgatcaggc tcgaagcgtt ctggaggaac tcgcggtacc ggaggtcgct ctccttctgc
     2521 gcatcgatga gttttgcatg ctctgaccgg tcctcgcaga ctatcacctg cccccgcgag
     2581 gggtccgccg ggtccatcgc ccggatgctc aggcggcagg ggatgagcgt cccgtccgct
     2641 cttttcaaca cggtatccgc gtggccccat ccctggtcgt cggaccggtg gcaaagctcc
     2701 ctgaaggcac gctcgtactc ctcgttcccg gggaagactg cctggacgcc ctgcccggcg
     2761 agcgcccccg cgtccatgcc gaggctgcgg gccattgtcg aattgaccca ttcgaagttc
     2821 ccgccccgca cctggcagat ccagagcggc gagacagaga ggatagccga gagcatgtcg
     2881 cagcggagga cctggtcacg ctcggctttc ttccggagca ccgcctggta gaggatgtcc
     2941 cgcatctcct gcacaggacc ccgcgagccc tccctctggc cgtgtacctc ggctgcctgg
     3001 tgcacgtcct cgatgaggag gctgtcccgg tcggtccggt ggtagaggat gaacgggatg
     3061 cctgcgtggg tccctttcag ttctttgagg agctcgactc cggtcatgtc ggccaggaac
     3121 tcgatcccgt tcacctcggg gaggcggtag tacgagacga tgatgtcgta tctcctccgg
     3181 cgggccatct ccagggcctg cttcgtggag tggacggcgt cgacccggat ctcccccatc
     3241 ctctcgaggt atgccctggt gctctcgagg gcgtcgctgt tgtcgtggac gagaagaacg
     3301 gtgatcatgg ctgacggctg ctccggtgga tcgttcctgg gagtatggga aaaggtagga
     3361 gatgccagaa tttaataatt attggtccga aatccgggat tgagcgcgca ggcggcggaa
     3421 tttaaagaaa tggtcttacc tcccccttcc ttttcccctg ctgacgccgg catctccgcc
     3481 ggactgcatc ctgaagagcg ctgcggcgac ctcgagcgac gtgtgctcgt cccccatcac
     3541 ctgctcgacc ctcgcgatga acggctccag gtcgccggcg gtgatgttct cacggatcct
     3601 ggcgatcatg ttgacgatgc gccgctcgcg gaggtcctca tcggccggga cggcgtgacg
     3661 ggcgatgcgg atcttcgcaa acttctggat gtcccggagt ttccacatct ctttcccgga
     3721 gacgaaggtg aaggccttgc cggacttgcc ggccctgccg gtccggccga tgcggtggat
     3781 gtagtactcg gggtcctggg ggacgtcgta gttgaagacc accccgacct cctcgacgtc
     3841 gatcccgcgt gcggcgacat cggttgcgac caggatgtcg gaggcgccgg tccggaaccg
     3901 ggacatcacc cgctctcgtt ccttctggtc catgtcgccg tggagcgcct cggcgaggta
     3961 cccgcgggcc tggagctggc cgctgacctc gtccaccttc cttttcgtgt tgcagaagac
     4021 gagcgagagc tttgggttgt agaaatcaag cagccgggag atgacctcga ccttgtccga
     4081 ctcccgcacc tcgaagtagc gctgctcgat ggcggggacc gccaggcggc cgtgcaccac
     4141 cttgaccatg cgcgggtctt ttaaatactt ctgggcgagc gcgaggatct cctttgacat
     4201 ggtggccgag aagaggacag tctggcgttc ggtcggggtg cgtttcagga taatctcgat
     4261 gtcgtcccga aagcccatgt ccagcatctc gtcggcctcg tcgagcacga cgagccggat
     4321 ccccgagagg ctgacggtgc ggcggtcgag gtggtcgatc aggcggccgg gggtggcgat
     4381 gacgacctgc acaccttttt taagtacaga gatctggcgc tcgatgggct ggccgccgta
     4441 gacgggcagg atggtgatcc cgcgcaggtg ccgggcgagc ttcctcaact cctccgagac
     4501 ctggatggca agctccctcg tggggcagag gacgatggcc tggacctccg ggactttctg
     4561 gtccacctgc tccaggagcg gaatgccgta ggcggcggtc ttcccggtcc cggtgtgggc
     4621 ctggcccacc atgtcgtggc gttcgcggat gacagggatg gcgagcgact ggatgggagt
     4681 gggctcctcg aacccctggt ccaggactgc ccgcatgagc ggctcgggga ggtcaaggtc
     4741 gacaaatgag atggttttca tgtacatgtt ccgtagcgtt ccgggcattc tcccggggag
     4801 cccgcgcagt attcactggt ctgcagtggt cccccggatc gtgctgcagg aataatgccc
     4861 ccttaccctt tataggcatt tgcgatgggt atctctcctc caccccgatg gagtgctact
     4921 ccctgataac caccgaagcc ttctcgtcga acgggttggt gcggagcgca agggagtaga
     4981 ggtaggggta gtggcgcttc aggtggccca ggtacacgac ccattcgagg accaggaggc
     5041 cgtagacccg gttcaggtct ccggagaggt gcgccgtgtc ggcccccggg agaccgcaga
     5101 gtacttcccg gtgcgagagt tcctgccaga ggtggaacaa agcctggagc aggtcggtga
     5161 acttcgagtg ctcgtcgagg tcggggttct ggaggaggac ggaaaggacg tcgattttct
     5221 cccccagggc gtcccggagg gttgcgaggt cgagttcccg gcagtcgaga tggtatgacc
     5281 gggacgcgag cgcgccggtg atcccggcgg acgcggggga gtctgcgaat tttggccctg
     5341 acagtaggcg ggagaggtcc tggcggccca ggtcaccccg tgcacaggtc gcaaggagcg
     5401 aggtgcccac ctgcgtgaag aagatcccga ccagcatcat cgtcttctgg cgccggcggg
     5461 cccgctcctt ccggaaggcg atgtgctcga ccacgtttgc gacgaggcct acgaagcacc
     5521 ccacgccggc gacgatcacg acgaccgcaa gcaccttccc ggcctctgtt gccgggtgaa
     5581 ggtccccgta tcccaccgtc gcgatggtca cgatggtgag gtagaacgag tcgagcggcg
     5641 agaacccctc cagcagcgag aacccggcga caccgatgac cacgacagag accaggagga
     5701 cgatataggc ctggagcctc cgttcatacg agcccatctt gtggaagggt cgccctgcat
     5761 gggtgataaa cctcacctcc gcggcgcggg cctccgcacc cttcatcacc tcgcacaccc
     5821 tccgtacctc tgttgaaacc atgacggaca ccttcgaggg agtgatacgg gaactgaagg
     5881 ccaaaaggaa agaggagcgc tggaagaact acgacacctg gaagcgatcc tgctgctgcc
     5941 cggactgccc gagctacaac gagtgcgcat cgggaggccg ggaactcctc tactgtatcc
     6001 tcgggatgag catccagtgt gtcagggagg accggcactg catctgcaaa gagtgccccc
     6061 tctactccac gctcgggctc tcgggaaaag acttctgcat gaaggggagc gaggcggcga
     6121 tccggtacga gcggagcgtg gagtgaggag ggggggtttc ctattgggaa accgaatgtt
     6181 tcctgttggg aaacaatttt tgacatatta cgcagatacg tcgatgactt tactgccctg
     6241 gtaatgttaa gcccgttcgt tcagcgctgc aacacaccct gaaattgcat cccggatatt
     6301 ttcaagtgcc tcctcgattg tctctccctg agagtggcac ccagggagag acgggcaact
     6361 gactatgaac ccgccatctt cactgtccgg tgtgatgact accttgaaga tcataatctt
     6421 tgacctggtt atactctgac aatttgaaaa tgtctctgga tattacatat gcgaaaatga
     6481 aaaagaaaag ttatgacggg tcaaaaaatc ctaatttctg aaataacatg aattgcagga
     6541 ttcttctgtt tcttcattca tcactgaatc catcttctcc ctcatattct tcctcttcct
     6601 gctgtttcat tttgtcctgg acgaagatca gccaccccac tg
//
LOCUS       DAOQYR010000107         6549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_12138_length_6549_cov_9.252849, whole genome shotgun sequence.
ACCESSION   DAOQYR010000107 DAOQYR010000000
VERSION     DAOQYR010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12138_length_6549_cov_9.252849"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(284..1531)
                     /locus_tag="PKM87_10265"
     CDS             complement(284..1531)
                     /locus_tag="PKM87_10265"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNQ30491.1"
                     /translation="MTDQNLFEMVKQHLCSCSTDLVMDRNVEAVLKMPRREMHASIPV
                     RMDDGKIRAFQGFRVQYNDALGPTKGGIRFHPDETIDTIRGLAALMTWKCALHNLPLG
                     GAKGGIVCNPKELSEHELERLSRAYVQVMFPYLGPDRDIPAPDVYTNPAVMAWMMDEY
                     SKIAGKTSFGSFTGKPVGLGGSEGRLDATARGGWYIVREAARDIGMNISGAGVAVQGF
                     GNVGSHAALIGQEVFGARVVAVSDSRGGVYNPDGLAVHELVSHKAATGSVQHFPGATN
                     ITNEELIELPVEILVPAALENVISSRNAPQVRAGIVAEFANGPVDNDADEILFEKGTT
                     VLPDFLTNGGGVIVSYFEMVQNFNMDQWDEGKVHRKLEKKMTDAYREVHDLASQNNVP
                     LRKAAYSIAVARVVEAMRKRGWV"
     gene            complement(1644..2495)
                     /locus_tag="PKM87_10270"
     CDS             complement(1644..2495)
                     /locus_tag="PKM87_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNQ30492.1"
                     /translation="MTARNLITDAQNTWCPGCGNFVIQFALKNTIAALEKEGVDTDRV
                     VLVTGIGCHAKMADYLNVNSFYSIHGRTLPVATAIKMANPELVVIACAGDGDCYAEGL
                     DHLIFAAKRNTDITLLVHNNRVYGLTTGQYTPTSPLGFRGRSTPSGTVEEPFNPLEIM
                     LASGATFVARGCTRKMALLQRTIMEGIRHPGFSFIDVLQVCASYFNLSDYYDRSVYEI
                     RDHDERDFEAACQKAREWDYNTDAPIGLGTIYRAKRPTLEERLSAIRGTETDRDATIR
                     NVLEKRT"
     gene            complement(2492..4144)
                     /locus_tag="PKM87_10275"
     CDS             complement(2492..4144)
                     /locus_tag="PKM87_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNQ30493.1"
                     /translation="MEELSVLIGGKAGDGINSAGMVVAHLFNRMGYRVYMYFDYPSLI
                     KGGHNFAIVRAAKEKIGAHRSQVDFILALNQDAVDFHKDMVHGKTVVLSDRSKVRAEG
                     IGIDLPQILKEEKAPPVMGNSCIIGAFAKAAGIEWEVLEEVFARQIPKALPTNLRVAR
                     RGYDAASECCRIEPLGSSPLPVISGNEAIGLGLIHGGLDAYVAYPMTPTSNILHFMAE
                     VAGAHDLLVFHPENEIAVMLMALGLGFAGKRTAAGTSGGGFCLMTEGFSLSGMSEIPV
                     VVVLGQRAGPSTGMPTYTAQSDLHFALHAGHGEFPRLVVAPGDAEQAYAWSDIALRLS
                     WKYQVPAIILCDKTLCEGFYSFISPDDTMPRVDPGPVGSPGPGYIRYGLTESGVSPML
                     FPPEKDTVIKVNGYTHDQNGITTEKAGLASRMSEKRGRKGSALAEEVLRFKPVESGGV
                     RQSETTLLCWGSTIGVCREVAAGLGLRLVQPVVLSPLPLQALHDALRGTKRLIAVEEN
                     AEGQIAMLLARHGIRLDSHIRRYDGRPFAREELEARVREALA"
     gene            complement(4422..4700)
                     /locus_tag="PKM87_10280"
     CDS             complement(4422..4700)
                     /locus_tag="PKM87_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1894 domain-containing protein"
                     /protein_id="HNQ30494.1"
                     /translation="MGCIESLKYEIVLRGASFAECRGYVEEHCSERVIVDPGFKIFEK
                     PIIGVPPVVIGLDGDVITFPYTKPCYGTFLLKTENPPEAARIRSLGKK"
     gene            complement(4679..5155)
                     /locus_tag="PKM87_10285"
     CDS             complement(4679..5155)
                     /locus_tag="PKM87_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1890 domain-containing protein"
                     /protein_id="HNQ30495.1"
                     /translation="MAAGTEKKDALLVLGCPQVPVQSPVALYLMQRLRMLGISPVVAG
                     NQAARMQVEVSDPLHHYSGEMVDLDTCIADLAEGRRSYSYYMIFVHNDAGVSYAATVQ
                     AITGKKVVAILYGEHFREVGETIGFPCEKVAAKAVHNPMPLKKKIDEVLPWVVSNL"
     gene            complement(5203..5964)
                     /gene="fdhD"
                     /locus_tag="PKM87_10290"
     CDS             complement(5203..5964)
                     /gene="fdhD"
                     /locus_tag="PKM87_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNQ30496.1"
                     /translation="MNTVTIRGIQVRNGRPREIEDTVVRESRFSLYLNGTYFTSMVAS
                     NDQLDELGAGFVTCQGISGRVDEVRVKGEEIHVIAPITGELLQEIISTGALGVRRPFL
                     PVTSSLTLTTDDVYRITGEIETETWRRTGGVHCSVLFHENELLVKASDLGRHSTVDKV
                     VGHAVIRGIDRSRCVLGCTGRQPRDMVTKAAHAGIPVVVSRAASTEQGIETAEKAGIT
                     LICFSRGDRFTVYTHPEKVRDLRGYLNQAGVRNGK"
     gene            complement(5961..6290)
                     /gene="cutA"
                     /locus_tag="PKM87_10295"
     CDS             complement(5961..6290)
                     /gene="cutA"
                     /locus_tag="PKM87_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNQ30497.1"
                     /translation="MNEKEQSRINVILVTSGRNEGPAIARHLVEKRMAACVNIAEIRS
                     CYRWEGRFCDDPEVLLIIKTAHEKVEEALAAIREVHPYTLPEMIVLPVVAGYPPYLAW
                     VEEETTR"
BASE COUNT         1322 a         2012 c         1769 g         1446 t
ORIGIN      
        1 atgaatatca cccggagcgt gccgaggctc acgtcgcata tcctcgccag gaagatgagc
       61 aggggaagta ttacccatgc atacacatcc gcagagatag tgaattccag cacttagcaa
      121 tcacccccgg acgatttgtc tgttctggac tccctcatca tctcatcctg agagcggatg
      181 ccagcccgtg ccacaggcca taccatgccg atgtaccgtg agatgccatt cctgataatt
      241 cccgcgtggc aatgactggg ggggaaggtc gatgtacagg ggattaaacc cagcccctct
      301 tcctcatggc ctccactacc ctcgcaacgg caatagagta cgccgccttc cggagtggca
      361 cgttgttctg ggacgccagg tcgtggacct cgcggtaggc atccgtcatc ttcttctcga
      421 gtttcctgtg caccttccct tcatcccact ggtccatgtt gaagttctgg accatctcga
      481 agtacgagac gatcacgcca ccgccattcg tgaggaaatc cgggagcacc gtggtaccct
      541 tctcaaagag tatctcatcg gcatcgttgt ccaccggccc gtttgcaaac tccgccacga
      601 ttcctgcacg gacctgcggg gcgttccggc tgctgatcac attctcgagc gccgccggga
      661 cgaggatctc caccggaagt tcgatcagtt cttcgttcgt gatgttcgtg gcacccggga
      721 aatgctgcac cgacccggtc gcggctttgt gggagaccag ttcgtgcacg gcgagcccat
      781 ctggattgta aaccccgcca cggctgtcac tcaccgccac caccctggcg ccgaagacct
      841 cctgccctat gagtgcggcg tgtgagccca cgttgccgaa cccctggaca gcaacacctg
      901 cccccgagat gttcatcccg atgtcccttg cagcctcgcg cacgatgtac catccacccc
      961 tggcagtggc gtcaagcctc ccctccgacc cgccaaggcc aacgggtttg ccggtgaagg
     1021 agccaaatga tgtcttcccg gcaatcttcg aatattcatc catcatccat gccattaccg
     1081 ctgggttggt gtagacatcc ggtgccggga tgtccctgtc cggcccaaga tacgggaaca
     1141 tcacctgcac ataggcacgg ctgagtcgct cgagctcgtg ttccgagagc tctttcgggt
     1201 tgcagacgat ccctcctttt gccccgccga gagggaggtt gtgaagggcg cacttccagg
     1261 tcatcagggc ggcaagaccc cggatggtgt cgatagtctc atccgggtgg aacctgattc
     1321 cgccttttgt aggaccgagt gcatcgttgt actggacacg gaacccctgg aatgcccgga
     1381 tcttcccgtc gtccatcctt acagggattg atgcatgcat ttcccttcgc ggcatcttca
     1441 gcactgcctc cacgttcctg tccatgacaa ggtcggtcga gcatgagcag agatgctgct
     1501 tgaccatctc gaagaggttc tgatcggtca ttcgtttccc cctcttacga agacattcag
     1561 agccatccgg tatttaaagg cacggaaatg ccgcaccatg ccagcaggta cggcttggtt
     1621 gagtgaatga gagccattca gcttcacgtt ctcttctcca gcacattccg gatggtggca
     1681 tccctgtccg tttcggtgcc ccggatggca gagagccgct cttccagggt gggtctcttc
     1741 gcccggtaga tcgttccaag gccgatcggt gcatccgtgt tgtaatccca ttccctggct
     1801 ttttggcagg cggcctcaaa gtcccgctca tcgtggtccc tgatttcgta tacactcctg
     1861 tcgtagtagt cagacagatt gaagtaggat gcgcatacct ggagcacgtc gatgaaagaa
     1921 aagccgggat gacggatgcc ttccatgatc gtgcgctgca ggagagccat cttccgtgtg
     1981 catcctctcg caacgaacgt ggcgccggat gcaagcatga tctccagggg attgaaaggt
     2041 tcctcaacag tccccgaagg cgttgaacgg cccctgaacc cgagcgggga agtcggggtg
     2101 tattgccccg tggtcagccc gtagacccgg ttgttgtgga caaggagggt gatgtcggtg
     2161 ttccgttttg cggcaaagat gaggtggtca aggccttcgg cataacagtc cccgtcacct
     2221 gcgcaggcaa tgaccacgag ctcggggttg gccatcttga tcgcggttgc aaccggcagg
     2281 gtgcgcccgt ggattgagta gaagctgttc acgttgaggt agtccgccat ctttgcatgg
     2341 caccctatcc cggttacgag cacgaccctg tcagtatcaa ctccctcttt ctcaagggcc
     2401 gcaatggtat ttttcagggc gaactggatg acaaaattgc cgcacccggg gcaccaggtg
     2461 ttctgggcat cggtgataag gttcctcgca gtcatgcgag cgcctccctg acgcgtgctt
     2521 ccagttcctc gcgcgcaaac ggccttccgt cgtaccgtct gatatgagag tccagcctga
     2581 tcccgtgccg tgcgagcagc attgcgatct gcccctccgc gttctcttct accgcaatca
     2641 gccttttcgt cccgcgcagc gcatcatgca gggcctgcag agggaggggc gagagcacga
     2701 ccggctgcac cagccggagc cccaggccag ccgcaacctc gcggcagact ccgatcgtgg
     2761 acccccagca gaggagcgtg gtttccgatt gcctgacccc gcctgattct accggtttga
     2821 accggagaac ctcctctgcc agcgccgatc cctttcgccc cctcttttca ctcatccggg
     2881 aggcgagacc cgccttctca gtggtgatcc cattctggtc gtgggtgtag ccgttgacct
     2941 ttatcaccgt atccttttcg ggaggaaaaa gcattggcga gacgccgctc tcggtcagcc
     3001 cgtacctgat atacccggga cccgggctcc cgacagggcc tggatcaacc ctgggcattg
     3061 tgtcgtcagg gctgataaag ctgtagaacc cctcgcagag cgtcttgtcg cagaggataa
     3121 tggccgggac ctggtatttc caggagaggc ggagcgcgat gtcagaccag gcataggcct
     3181 gttctgcatc ccccggggcc accacgaggc gggggaattc tccatggccg gcgtgcagtg
     3241 caaagtgaag gtcggactgt gctgtatagg tgggcatccc ggtgcttggg cccgcccgct
     3301 gcccgagcac caccacgacc ggaatctcgc tcatgcctga gagtgagaac ccctcggtca
     3361 tcaggcagaa gcctcctccg gaagtccctg ccgcggtcct cttcccggca aatccgaggc
     3421 cgagcgccat cagcatcacc gcaatctcgt tctcggggtg gaagaccagg agatcgtgcg
     3481 ccccggcaac ctcggccatg aagtggagga tattggatgt gggggtcatc ggatacgcga
     3541 cgtaggcatc gaggccaccg tggatgaggc caaggcctat cgcctcgttc cccgagatca
     3601 ccggcagtgg agacgagccg agcggctcaa tcctgcagca ctcgctggcc gcgtcatagc
     3661 cccggcgcgc caccctgagg tttgtgggaa gcgcctttgg gatctggcgt gcgaatacct
     3721 cctcgagcac ctcccactcg atccctgcgg ccttcgcaaa tgccccgatg atgcacgagt
     3781 tccccatgac cggcggggcc ttctcctctt tcaggatttg tgggagatca attcctatcc
     3841 cctcggccct gaccttcgag cggtcagaga gcaccacggt cttcccgtga accatatctt
     3901 tgtgaaagtc cacagcatcc tggttcagtg cgaggatgaa gtcaacctgt gaccggtgcg
     3961 ccccgatctt ctccttcgca gcccgaacaa tcgcaaaatt atgccccccc ttgatgaggg
     4021 acgggtagtc aaaatacatg tacacacggt atcccatccg gttgaagaga tgggcaacca
     4081 ccataccggc actgttgatc ccgtcaccgg cctttccccc gatgagaact gataattcct
     4141 ccatgatcgt gaagagcacc ccgaacaaca ctctcttttc agggaaagat ataggttctt
     4201 cccaaaaaac cacacgattc cctttcaaga cacatgctca taagatgaaa acaagaatga
     4261 tgcaatttgc aacaagcccg acgcatgaaa atgccgcagc tattttcata ggaatcgctt
     4321 atgacggccc caccgtagaa tataaatgat gaaaatcacg aagtgatttt cattcgaaat
     4381 cgcaggtgcg atttcctata aaaaaaaact ggatggtgat gttatttctt cccaagggac
     4441 cgtatccggg ccgcttcggg agggttttca gtcttgagaa ggaacgtgcc atagcagggc
     4501 ttcgtatacg ggaaagtgat cacgtccccg tcaagcccga tgacgaccgg aggcacccct
     4561 atgataggtt tctcgaagat cttgaacccg ggatccacga tcacccgctc actgcagtgc
     4621 tcctcaacat agcccctgca ttctgcaaac gatgcccccc tgaggacaat ctcgtacttc
     4681 aaagattcga tacaacccac ggaagcacct cgtctatctt ctttttgagt ggcatgggat
     4741 tgtgcactgc ttttgcagcg accttctcgc aggggaagcc gatagtctct ccaacctccc
     4801 tgaagtgctc cccatagagg atggctacca ccttcttccc ggtgattgcc tggacggttg
     4861 cagcatacga gaccccggca tcgttgtgca cgaagatcat gtagtacgaa tagcttctcc
     4921 ttccctccgc caggtccgcg atgcaggtat cgaggtccac catttcccct gaataatggt
     4981 ggagcgggtc tgatacctcg acctgcatgc gggcggcctg gttgcctgcg acgaccggtg
     5041 atatcccgag catccgaagc ctttgcatga ggtacagggc aacgggggac tggaccggga
     5101 cctgggggca tcccaggacc aggagcgcgt ccttcttttc tgttcctgcg gcgatgatga
     5161 atctcctccc tgcctgacta attaacgatc cccatggcta tgttacttcc cattccggac
     5221 acctgcctga ttgaggtacc cccgaagatc cctgaccttt tcaggatgcg tgtagacggt
     5281 aaacctgtcg ccgcgggaga agcagatgag ggttattcct gccttctctg ccgtttctat
     5341 cccctgctct gtcgacgccg cacgggagac gacaacgggt atcccggcat gcgcggcctt
     5401 tgtcaccatg tccctcggct gccttccggt acagccaagg acgcacctcg acctgtcaat
     5461 acccctgatc actgcatgcc ccaccacctt gtcgaccgtg ctgtgccttc caaggtcgct
     5521 tgccttcacg agaagctcgt tttcgtggaa gaggaccgag cagtggacgc cgccggtccg
     5581 ccgccacgtc tcggtctcga tctcccccgt gatacggtac acgtcatcgg tggtgagggt
     5641 aagagacgat gtgacaggga gaaaaggcct cctgaccccc agcgcccctg tggatatgat
     5701 ctcctggagc agttctcccg tgatgggggc aatgacatgg atctcttctc ccttcaccct
     5761 cacctcatca accctgccgg agattccctg gcacgtcacg aaacctgcac cgagttcatc
     5821 gagctggtcg ttgcttgcaa ccatactggt aaaatacgtc ccgttcaggt aaagggagaa
     5881 ccggctctcc cgcaccacag tgtcctctat ctctcggggc ctgccattcc tgacctggat
     5941 gcccctgatg gtgacggtgt tcatcgagtg gtctcctctt caacccaggc aagataggga
     6001 ggataccctg caacgaccgg gagcacgatc atctcgggga gggtgtatgg atgcacctcc
     6061 ctgatggcgg caagggcttc ctcgaccttc tcgtgtgcag tctttatgat gagaagcact
     6121 tcgggatcat cgcagaacct cccttcccag cgatagcagg acctgatttc cgcgatgttc
     6181 acgcatgcag ccatcctctt ttccacaagg tggcgggcga ttgcagggcc ctcatttcgc
     6241 ccggaagtga cgaggatgac attaattcgt gactgctcct tttcattcat ctcctgttca
     6301 ccttcatgat ccgggacaca cccgtgaaac cggccgggga atgtgcatgc tctttactac
     6361 ccgggcgcgg gatataagag tattgcttca attctctcaa ttaatgggat atttcaggat
     6421 ctgaacgcat cagcgcctat tttggacgac agcccgtggt gatcattaat gaatgagcag
     6481 gccctatact cctgcacatg tacgcggaac gaatggagat gctccctccc tacctgttcg
     6541 ccaggatag
//
LOCUS       DAOQYR010000108         6543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_12151_length_6543_cov_8.750308, whole genome shotgun sequence.
ACCESSION   DAOQYR010000108 DAOQYR010000000
VERSION     DAOQYR010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12151_length_6543_cov_8.750308"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..677
                     /locus_tag="PKM87_10300"
     CDS             12..677
                     /locus_tag="PKM87_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF120 domain-containing protein"
                     /protein_id="HNQ30498.1"
                     /translation="MILPEELQCLKAIALLGGCRGPVRVSSQSLGATLGTSPQTASRR
                     LQSLERQRLVSRTIAADGQDIMVTPLGEAELRKEYQEYSRIFSDHNLGYVLTGTVISG
                     IGEGKYYMSLEPYKEQFNRVLGFEPYPGTLNIRLFPSSIPLRKKIEALDWIRIKGFSS
                     DGRTFGDARCLPCRIGDISCGIVVPGRTHYPDDVIEVIAPVALRRKLGVEDSDTVTVE
                     VGE"
     gene            674..1369
                     /gene="ribB"
                     /locus_tag="PKM87_10305"
     CDS             674..1369
                     /gene="ribB"
                     /locus_tag="PKM87_10305"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HNQ30499.1"
                     /translation="MIGNALAELRDGKFVLLYDFDNRERETDFAIRSDAVTPRHIFRM
                     RKDGGGLICTAVHPVAARRLGLPFASDALRAMAVAEREGDIPYDRKNHSSFSLWVNHR
                     DTFTGITDRDRALTVNAIAEQVKCALNGGGAQFHEIFRTPGHMALLRAADGLLSVRRG
                     QTELSIAMAEMAGITPAVTICEMLDDESGYALSKEDAMAYAKKHGMVFVEGNEVLEAW
                     DAFVSGGKRGIPE"
     gene            complement(1488..1649)
                     /locus_tag="PKM87_10310"
     CDS             complement(1488..1649)
                     /locus_tag="PKM87_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30500.1"
                     /translation="MKDEEIQEMVKNLDLKTLKEAAKAAGIKPGRCPTKTSIAKMLPE
                     ETLKMLAKK"
     gene            complement(1721..2293)
                     /locus_tag="PKM87_10315"
     CDS             complement(1721..2293)
                     /locus_tag="PKM87_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleic acid-binding protein"
                     /protein_id="HNQ30501.1"
                     /translation="MPGRRDGVFEREPARRVFAQELRESRVHFREGQDEKSPTFVLLP
                     TGARCNRVFFAGTLTEKKKQGEQNMFYRARVVDPTGTFFVMAGSYQPEAMQQIARLEP
                     PCFVAVVGKPNVYQTPDGAVLISIRCESITQVDKDIRDEWVLDCARATLDRIEKSAGT
                     PDGDRARREYTIDPGLFRKMVYEALAQLKI"
     gene            complement(2314..3549)
                     /locus_tag="PKM87_10320"
     CDS             complement(2314..3549)
                     /locus_tag="PKM87_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HNQ30502.1"
                     /translation="MDFSHTAERISQKITSHGHSVEKSGIEAKLKRLVEEFGVPPAEA
                     ERTVLNEFAREYDIPGFQGSGGGGAGDQKALSLLANGEWVTVECKVVALTAAPSPSIA
                     QTGIVADESAAVRFVVWSRANAPVLEEGKWYRLESAVVDQYKGMLNLKVHSGTTIREV
                     SHEGTLKPTITPIKDLAPGVACIRGKVVQEWEATHDRILQSGMFADETGAIRFVIWKE
                     EGRERLNTGGIYTIYYATVDDFNGRLSLTLNTATTVPEEGDIEVNTGNSLFEGAFVHL
                     SPGSGLVRRCPVEGCNRVLSRQNYCPVHEIQPNYHYDLRIKGWLDNGEETREVLIQRG
                     ETEGLVGMSLDQAKELAENNPLGMDEVFLRFREALLGRYLSCRGREIDNRLLVSSCEL
                     LHYTPDQLADLLNRAGGSL"
     gene            3681..3875
                     /locus_tag="PKM87_10325"
     CDS             3681..3875
                     /locus_tag="PKM87_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HNQ30503.1"
                     /translation="MAITADSTIYELLKAKPESAEVLFKFGMGCVGCAIARGETIKEA
                     AMAHGIPLDELVSALGIAQG"
     gene            complement(3882..4760)
                     /locus_tag="PKM87_10330"
     CDS             complement(3882..4760)
                     /locus_tag="PKM87_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HNQ30504.1"
                     /translation="MRYYIRDLTLILRGTFRAAGTGVPGGLATVPTLLLHAGGERGAV
                     PDPTRKLEGIIHRQGLPPEYLGLMSGVRAECLCIMQYDFLDVFLAAGALQGDHGGRVS
                     VNMIVYSREGMTDAALLETIMTGTAAREGILHSLGRPTGGTPSDGIVVACEGEVEHGG
                     GGTMTEIGMRLSEAVRFGVQEALARHEGRITRSRPSFFIYSRFQGDHWVEWLPEDCPY
                     YPCHFPGQRCEFCYCPFYPCGDESLGQWVKSASKNDLVWNCSTCTLLHEPEIADYLLA
                     NPEAPLAELKRKKWMH"
     gene            complement(4957..6204)
                     /gene="thiC"
                     /locus_tag="PKM87_10335"
     CDS             complement(4957..6204)
                     /gene="thiC"
                     /locus_tag="PKM87_10335"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF009895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNQ30505.1"
                     /translation="MVLTPSSPSPQRPLDKESIRLVAKAEKVDPTVLARHVGDGSVVI
                     MRRGDRCVGIGRGLRIKVNVNIGTSNVRSVPHEEVKKAVVAEHCGADTISDLSMGRDV
                     EETRRLIFSHTSVPITTVPVYQAAAEQGIEGMDARDILDTVRRQAREGVSSMVVHCIT
                     RDILEACRREGRVLGLVSKGGSITAAFMLHHGCQNPFIEHFQEILGIMRSCGIVLSLG
                     NSVRSGCIGDGWDQAQDEELHTNLALAEEAHRAGVQVIIEGAGGHIRYDRIASTIRRY
                     RDASRFPLFVAGPLPTDIALGLDHIAGCVGASAAGAAGADYLCCITPAEHLGLPTPAQ
                     VREGLLAWRIAAHIGDLARLGLDMQDRKMAEKRALMDRKGQISLALDPEAARTSGADE
                     ETCTMCGDFCALRLMRRYLKGPR"
     gene            complement(6311..>6543)
                     /gene="nrdD"
                     /locus_tag="PKM87_10340"
     CDS             complement(6311..>6543)
                     /gene="nrdD"
                     /locus_tag="PKM87_10340"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HNQ30506.1"
                     /translation="GDTHLQIMCPLDHCHCSHEIISTIEYCPLCGAPVCPECGSHDVV
                     QISRVTGYLQEVSGWNAGKQQELKDRVRYTLA"
BASE COUNT         1221 a         2050 c         1964 g         1308 t
ORIGIN      
        1 attctgatat aatgatcctt ccagaggagc tccagtgcct caaggcgatc gcgcttttag
       61 gcgggtgcag gggaccggtc agggtctcct cgcagtccct cggggcgacg cttgggacaa
      121 gcccccagac cgcgtcccgc cggctccagt ctctcgagcg ccagagactg gtgagcagga
      181 cgatcgccgc agacggccag gacatcatgg tcactcccct gggcgaagcg gaactgcgca
      241 aggagtacca ggagtacagc cggatcttct cggaccacaa ccttggatac gtactgacgg
      301 gcaccgtcat cagcgggatc ggcgagggca agtattacat gagcctcgag ccatacaagg
      361 agcagttcaa ccgggtcctt ggtttcgagc catacccggg tacgctgaac atccggctct
      421 tcccgtcgag catccctttg agaaagaaga tcgaggccct cgactggata cggatcaagg
      481 gcttctcatc ggatgggagg acgtttggag atgcccggtg cctcccgtgc cggatcgggg
      541 atatctcctg cggcatcgtg gtgcccgggc ggacgcatta ccctgatgac gtcatcgagg
      601 tgatcgcgcc ggtcgcgctc cgcaggaagc tcggtgttga ggacagcgac actgttaccg
      661 tcgaggtggg ggagtgatcg gaaacgccct tgcggagctg cgggatggca agttcgtcct
      721 cctctacgac ttcgacaacc gggaacgtga gaccgatttt gcgatccgct cggatgccgt
      781 cacaccccgc cacatcttcc ggatgagaaa ggatggggga ggcctgatct gcaccgctgt
      841 ccacccggtc gcggcccggc gcctcgggct ccccttcgca agcgacgccc tccgcgcgat
      901 ggcagtcgcc gagagggagg gagatatccc ctacgaccgg aagaaccatt cctcgttctc
      961 gctctgggtg aaccaccgcg acaccttcac cgggatcacc gacagggacc gtgcgctgac
     1021 tgtgaacgcc atcgccgagc aggtgaaatg cgcgctgaac ggcggaggcg cacagttcca
     1081 cgagatcttc cggacaccgg gccacatggc gctgctcagg gctgcagacg ggcttctctc
     1141 cgtccggagg ggccagaccg agctctctat cgcgatggcg gagatggcag gcatcacccc
     1201 cgcggtcacc atctgcgaga tgctggacga cgagagcggg tacgccctct cgaaggagga
     1261 cgcgatggcc tacgcaaaaa agcacggcat ggtcttcgtg gagggaaacg aggtgctcga
     1321 ggcctgggat gcgttcgtct ccggagggaa gcgcgggatc ccggagtaac agccacgcga
     1381 ttcctcttca cgggcaagag acctctcttt tttcccgcat gaatcctgaa ttatcattgc
     1441 aggaaaagcg tccacgcggt ttttttgagc aaaaaaaggt gcgggagtta ctttttggcc
     1501 agcatcttca gagtctcttc agggagcatc tttgcaatcg aggtcttcgt ggggcagcgg
     1561 cccggcttga tacctgctgc ctttgcggcc tcttttagtg tcttgaggtc gaggttcttc
     1621 accatctcct ggatctcttc atccttcata aggtcaccag taagatctac cctcgcgccc
     1681 tggagataaa agttgttgat cccgggagga tcccgggaaa tcagatcttc aactgcgcga
     1741 gcgcctcgta caccatcttc cggaatagcc ccgggtcaat ggtatactcc cttctcgccc
     1801 ggtccccgtc cggggtcccg gcagacttct cgatccggtc aagggtcgcc ctggcgcagt
     1861 cgagcaccca ctcgtcccgg atatccttgt caacctgggt gatggactcg cagcggatgg
     1921 agatcagcac ggccccgtca ggcgtctggt acacgttcgg ttttcccacc accgccacga
     1981 agcatggagg ctcgagcctt gcgatctgct gcatggcctc cggctggtag ctgcccgcca
     2041 tcacgaagaa ggtcccggtg gggtccacca cccgcgcccg gtagaacatg ttctgctcac
     2101 cctgcttctt cttctcggtg agcgtgcccg caaagaagac ccggttgcat cgcgccccgg
     2161 tggggaggag cacgaacgtc gggctcttct catcctggcc ttccctgaaa tgcacccggc
     2221 tctctcttaa ttcctgtgca aagacacggc gggcgggctc gcgttcgaag acgccgtccc
     2281 tcctcccggg cattcccttc gcgttcccgt tggtcacagc gaaccacccg cccggttcag
     2341 gagatcggcc agttgatcag gcgtgtagtg gaggagctcg caggatgaga caagaagccg
     2401 gttgtcgatc tcccgtcccc tgcaggaaag atacctcccg aggagagcct cgcggaaccg
     2461 gaggaagact tcgtccatcc cgaggggatt gttctccgcg agctcttttg cctggtcgag
     2521 gctcattccc accaggccct cggtttcacc cctctgtatg aggacttccc gcgtctcctc
     2581 cccgttgtcg agccagccct tgatccggag atcgtagtgg taattgggct ggatctcgtg
     2641 cacagggcag taattctggc gggagaggac ccggttgcat ccctcgacag ggcagcgcct
     2701 gaccagcccg gaaccaggtg aaaggtgcac gaacgctccc tcgaacaacg agttccctgt
     2761 attgacctcg atgtccccct cctccgggac cgtggttgcg gtattcaggg tgagcgagag
     2821 gcggccgttg aagtcatcca ccgttgcgta atatatggtg tagatacccc ccgtgttcag
     2881 gcgttccctg ccctcctcct tccagatcac gaaccgaatg gccccggtct catctgcgaa
     2941 catgccagac tgcaggatcc ggtcgtgggt ggcttcccat tcctggacca ccttgccccg
     3001 gatgcaggcc acgcccggcg caaggtcttt gatgggggtg atcgtcggct tgagtgtgcc
     3061 ctcgtgggaa acctccctga tcgtggtccc tgagtggact ttcaggttca gcatcccctt
     3121 gtactggtcg acaaccgcgg actccaggcg gtaccatttt ccctcctcga gcacgggcgc
     3181 gtttgccctt gaccagacca cgaagcgcac cgcggctgac tcgtcggcca cgatccctgt
     3241 ctgggcaatt gagggggagg gcgcggcggt gagcgcaacc actttacact ccacggtcac
     3301 ccactctccg tttgcaagca gggacagtgc cttctggtct cctgcccccc cgcccccgct
     3361 tccctggaac ccggggatat cgtattctct cgcgaattcg ttcagcacgg ttctctcggc
     3421 ttctgccggc gggacaccga actcctcgac aaggcgctta agttttgcct ctattccgct
     3481 cttctccacg gaatggccgt gggaggtgat tttctgggag attctttcag ctgtgtggga
     3541 aaaatccatt cttacatctc caggagatta gtcatattgc catgggtact taaaggatgc
     3601 gggtgggtgg tgaaactgcg atggcacgca agtaccacct gctgcaggtt tatatgcaaa
     3661 tacctgtaca ttctgagcgt atggcaataa ctgcagacag cacgatttac gaacttctga
     3721 aagcaaaacc cgaatctgca gaggtcctct tcaaattcgg catgggatgc gtgggatgcg
     3781 ccatcgcccg cggggagacc atcaaggagg ccgccatggc ccatgggatc ccgctggatg
     3841 agcttgtctc cgcccttgga atcgcgcagg ggtaatcctt actagtgcat ccattttttc
     3901 cgtttcagct cggcaagcgg agcctcgggg tttgcgagga ggtagtctgc gatctccggc
     3961 tcatgcagga gggtgcaggt gctgcagttc cacacaagat cgttctttga tgcgctcttc
     4021 acccactggc cgaggctttc gtcgccgcat gggtagaaag ggcagtagca gaactcgcac
     4081 cgctgccccg ggaaatggca tggatagtac gggcaatcct cgggcagcca ctcgacccag
     4141 tggtccccct ggaaccggct gtagatgaag aaggagggcc ggctgcgcgt gatgcgccct
     4201 tcgtgcctgg cgagggcttc ctgcacccca aagcggaccg cctctgacag acgcattcct
     4261 atttccgtca tggtccctcc gcccccgtgc tccacctccc cctcgcaggc cacgacaatt
     4321 ccatccgacg gagtaccccc ggtgggcctg ccgagtgaat ggagtatccc ctccctcgca
     4381 gcggtgcctg tcatgatcgt ctccaggagg gcggcatcgg tcatcccttc gcggctgtac
     4441 acgatcatgt tgacggacac gcgcccccca tggtcgcctt gtaacgcccc ggcagcgagg
     4501 aatacgtcca ggaagtcata ctgcatgatg cagaggcact ccgcccgcac tcccgacatg
     4561 aggccgagat attctggagg gagcccctgc cgatggatga tcccctccag tttccgggtt
     4621 ggatcgggga ctgcaccacg ctcacctccg gcgtggagga ggagcgttgg gactgtcgca
     4681 agtccccccg gaactcctgt gcctgcggcg cggaacgtgc cgcggagaat gagggtcagg
     4741 tcgcggatat agtatctcat gccaggggag gggtacggag gggtggttcg cccgggagaa
     4801 cacagggcgc gctctttgtt gcatgcaggc cgaaagaagg agggagtact cggcgttcca
     4861 tcagactgat tggttttcca tcctttcaag aaataccttt ggagcggggt gcgattccca
     4921 gcccgaaaag atggaatccc ccaatcgggg cctccatcac cggggcccct tcaggtacct
     4981 cctcatcaac cgcaatgcgc agaagtcccc gcacatggtg caggtctcct catcggcacc
     5041 ggatgtccgc gccgcctccg ggtcaagtgc cagcgagatc tgccccttcc tgtccatcag
     5101 tgccctcttc tctgccatct ttctgtcctg catgtcaagg ccgagcctcg cgaggtcccc
     5161 gatatgcgcg gcaattctcc acgcaaggag accctcccgt acctgtgcag gggtggggag
     5221 cccgaggtgc tcggcggggg tgatacagca gaggtaatcg gcccccgccg ctcctgccgc
     5281 actggcgccg acgcatccgg cgatgtggtc gagcccgagt gcgatgtcgg taggaagcgg
     5341 cccggccacg aaaaggggga aacgggacgc atccctgtaa cgccggatcg tagaagcaat
     5401 ccggtcatac cggatgtgcc cccccgcccc ctcgatgatg acctggaccc cggcgcggtg
     5461 agcctcctcg gcaagtgcga ggtttgtgtg aagctcttca tcttgcgcct ggtcccaccc
     5521 gtcacctatg cacccgctcc gtaccgagtt cccgagggag agcactatcc cgcaggaccg
     5581 catgatcccg aggatctcct ggaagtgctc gatgaacggg ttctggcagc cgtggtggag
     5641 catgaacgcc gcagtgatgg atccgccctt cgacacgagc ccaagcacac gtccctccct
     5701 cctgcacgcc tccaggatgt ccctcgtgat gcagtggaca accatggaac tcaccccttc
     5761 ccgggcctgt ctgcggaccg tgtcaaggat gtcccgtgcg tccatgccct cgatcccctg
     5821 ctcggccgcg gcctggtaaa ccgggacggt ggtgatggga accgaagtgt gcgaaaagat
     5881 gagtcgccgg gtctcttcca cgtccctgcc catcgagagg tccgagatag tgtcggcacc
     5941 gcagtgttcg gcgacaaccg cctttttcac ctcttcatga gggactgaac ggacattcga
     6001 ggtgccgata ttcacgttca ccttgatgcg aaggcccctg ccgatgccga cgcaccggtc
     6061 ccccctccgc atgatgacca cgctcccgtc tccaacgtga cgggcgagca ctgtgggatc
     6121 gactttctcg gctttggcta ccaggcggat ggattctttg tccaggggcc gttgtggaga
     6181 aggagaggag ggcgtcaata ccatcggaaa accacctccc accggccggt gcaggagggt
     6241 gaatctttcc aggaaaattc gtcacccgga gtagaaaaca ggaaggccat ccaggggaag
     6301 acgcggcaat tcacgcgagg gtgtagcgga cccggtcctt gagttcctgc tgctttccgg
     6361 cattccagcc ggagacctcc tgaaggtacc ccgtgaccct gctgatctgg acaacgtcat
     6421 ggctcccgca ttcggggcag acaggggcgc cgcagagagg gcagtactct atcgtggaga
     6481 ttatctcatg ggaacagtgg caatggtcca ggggacacat gatctggagg tgcgtatccc
     6541 cgc
//
LOCUS       DAOQYR010000109         6479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_12315_length_6479_cov_8.552927, whole genome shotgun sequence.
ACCESSION   DAOQYR010000109 DAOQYR010000000
VERSION     DAOQYR010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12315_length_6479_cov_8.552927"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PKM87_10345"
     CDS             complement(<1..278)
                     /locus_tag="PKM87_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30507.1"
                     /translation="MRLTAIVWGSEIPVLSTAAQDAGVSLHAHPTYRMRDPAYLDSCI
                     PKLTDSDVILLHPTNDAYWDTLIPALPPGVPVISFGHDQSFWTLSTVPL"
     gene            complement(282..1055)
                     /locus_tag="PKM87_10350"
     CDS             complement(282..1055)
                     /locus_tag="PKM87_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ30508.1"
                     /translation="MKLEVADLSFSYQDHRVLEEITLDVEGGELVGVLGPNGCGKTTL
                     IKCINHVHAPKGSVRFGGHDVARMGNTERARLFAYVPQALSIGMAMTVFESVLMGRRP
                     HVRWGVGDSDIGMVSAALERLKISHLAFRKVTQISGGERQKVVIARALVQDPMLLLLD
                     EPTSALDLRHQLEVMQIIRSVVDQKGIGGLMAIHDLNLAARYCDRVVVLHQGRIAGQG
                     KPSAILTEELIRNVYGVSARVGREDGYPLIVPLEPVASV"
     gene            complement(1052..2113)
                     /locus_tag="PKM87_10355"
     CDS             complement(1052..2113)
                     /locus_tag="PKM87_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNQ30509.1"
                     /translation="MDDEKEKIAGQYLRMQAKRLLFVGVLAVLIILLAVGSTIIGSAG
                     LTVEEVFTAVLAGLLPGSFSADPLAFTIVWDLRLHRVLFAVVAGFGLAIAGAVMQGVL
                     RNPLASPFTLGIASAATFGAAIAIIFVPTALSGEIALVVSAFMMSAFAAVAIYGLSRY
                     RGVSAESMVLAGIILMYLFGAMTSFLQYAGTTEQLQAVVFWMFGSLQNTSWEKLAIVT
                     LVVVVSTPLLVWRAWDLNALGMGDQVAQSLGVKVEQTRTVHMLVASLITASIICFTGT
                     IGFIGLVSPHITRLAIGGDHRYLIVASGFVGALILLGADSLARTILAPQIIPVGIMTA
                     FIGIPFFLYLFMRRKEEYW"
     gene            complement(2127..3335)
                     /locus_tag="PKM87_10360"
     CDS             complement(2127..3335)
                     /locus_tag="PKM87_10360"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ30510.1"
                     /translation="MNRFPLTVIVVFAVLCPIVLADAALPGDLDDNGVIAREELASSI
                     LGYLSQDTNISDAPISDLRDSAWVFVHWGGTARTVTDSSGKSLTLTRPLRRIIVMNSE
                     TLETMRSLGVTKERIVAVDKYIAQKPEFFPEYAGYPSVGSIWAPDYEKMLSLRPDALF
                     LYASVSKAECDEIERRVTLSRPDLTVFRIDCYRPETYLEDTARLAEIFGKEEEGARLA
                     AFYAGALAKVDAAASSSAPPEVYFETWNDFKSVARGSGYHDKVTMAGGANIFRDNPAE
                     YPEIDPEAVISRKPEVVIKLVGSSKYIFGGYSGENTTRFEDVRDTLLPRAGWSSLPAV
                     RDGRVYVLHNAIMGGPQYLIGVTYMARWFHPDSAGDLDPESLHRTYLQEFQGLDPGLA
                     RADRFVFPVK"
     gene            complement(3332..3856)
                     /locus_tag="PKM87_10365"
     CDS             complement(3332..3856)
                     /locus_tag="PKM87_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30511.1"
                     /translation="MKRGRLVTGIIPMIICAMLVQAAAAGIVERSIEPSEHSTYRVVL
                     TLPGETVAGIIEVLPSGMEVREVSLPPDQYRVDGATLFLAVIGEREITYFVHGQEDPA
                     GAITGTWTDYLSGEEGTVGGESDGTQGQGVPPAVPVTTGTAKAGASPLLLLGGAGLAF
                     IGVARCRARWRDEE"
     gene            4110..5066
                     /locus_tag="PKM87_10370"
     CDS             4110..5066
                     /locus_tag="PKM87_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ30512.1"
                     /translation="MIPIETSALTKYYGSLCAVDHLDLSIGNEIFALLGPNGSGKTTT
                     VLMLTTLLRPTAGSARICGHDIESDAGEVRRLISYVPQDMAVDIKLTGRENVTLFAKL
                     YGISHRDETIDRVLGMMALSERADDLVRTYSGGMRRRLELAQALVHEPKVLFLDEPTI
                     GLDVAARKKIWEHIRTLKSRGMTIFVTTHYMDEADHFCDRVAILDHGRVAAVDTPARL
                     KGMLGKDLISARVSGGFSGDLPAGIERLGEKENGEYLFSSDHASEKVPVLFRWLEEHG
                     AVIHSLSVREPTLDDVFLALVGSRDDVTGFDDHRFRTMLRRR"
     gene            5066..5791
                     /locus_tag="PKM87_10375"
     CDS             5066..5791
                     /locus_tag="PKM87_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ30513.1"
                     /translation="MKDILSYFERDMLRWIRGRVSVISALVVPAAWLVFVGLALPTTF
                     TDHYIDFITPGILVLTMLGASLQGGSLLIFDKILGFLNKFLALPSSRESILYGKILFI
                     TTRGLIQATVILILAFILGATFLSPWSIVLTYGILAVFGVLLSAFSTTVALYLSDHDA
                     YAAFNAMVSMPLFFTSSALMPYDVMPSWLAPLAYWNPVSYAIDAIRALQEGVLAWGRI
                     GALVLLAVLVVAISVRQFRKVTL"
     gene            6059..6385
                     /locus_tag="PKM87_10380"
     CDS             6059..6385
                     /locus_tag="PKM87_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30514.1"
                     /translation="MEMTVHEQSNSTLDTDRDTCLFRAPRDPDRIAYLRGKDLYEHRR
                     NDQPSLYYIHRWSLAPGERETLSLISPVMAGRFLESKGIFCPETGDFRGAAILRSWGY
                     GILEEF"
BASE COUNT         1364 a         1978 c         1897 g         1240 t
ORIGIN      
        1 agcgggacag tcgagagagt ccagaaggac tggtcgtggc cgaacgatat gacagggact
       61 cccggaggga gtgcggggat gagggtgtcc cagtaggcat cgttagtggg gtggaggagg
      121 ataacgtccg agtccgtaag tttcggaatg caggagtcaa gatacgcggg atccctcatg
      181 cggtatgtcg gatgggcatg cagggaaacc ccggcgtcct gtgcagcggt cgagagcacc
      241 gggatctcgc tcccccagac aatggcagtc aggcgcatgg atcagacgct cgcgacgggt
      301 tcgaggggaa caatcagggg atacccgtcc tccctcccga ccctcgcgga caccccgtac
      361 acgttgcgta tcagttcctc ggtgagaatc gccgagggct tgccctgccc ggcgatcctg
      421 ccctggtgga ggaccaccac cctgtcgcag taccgggccg ccaggtttaa gtcatggatt
      481 gccatcaggc ccccgatccc cttctggtcc accacagatc ggatgatctg catgacctcc
      541 agctggtggc ggaggtcaag cgcgctcgtc ggctcgtcga ggagaaggag catgggatcc
      601 tggacaaggg cccgggcgat caccaccttc tggcgctccc ccccgctgat ctgcgtaacc
      661 ttccgaaacg cgaggtggga gatcttcagg cgctcgagcg ccgcacttac cattccgatg
      721 tccgagtccc ccactcccca gcggacatgg ggcctcctcc ccatcaggac cgactcaaac
      781 accgtcatgg ccatccctat ggagagggcc tgggggacat aggcaaagag cctcgctcgc
      841 tctgtattcc ccatacgggc gacatcatgc cccccgaagc ggacgcttcc tttgggtgca
      901 tggacgtggt tgatacactt gatcagggtg gtcttcccgc acccgttcgg cccgaggacg
      961 ccgacgagtt cgcccccttc cacgtcaagg gtgatctcct cgaggacgcg atggtcctgg
     1021 taggagaagg aaagatccgc cacttccagc ttcaccagta ctcctccttg cgacgcatga
     1081 agaggtagag gaagaacggg atgccgatga acgccgtcat gatcccgaca ggaatgatct
     1141 ggggtgcgag gatggtccgg gcaaggctgt ctgcaccaag gagaatgagg gctccgacaa
     1201 agcccgatgc tacgatcagg tagcggtggt ccccgccgat ggcaagcctc gtgatgtgag
     1261 gggagacgag cccgatgaac ccaatggtcc cggtaaagca tatgatagag gcagtgatga
     1321 gggatgcgac cagcatgtgg acggtccggg tctgctcgac cttcaccccc aggctctggg
     1381 ccacctggtc gcccatgccg agcgcattga ggtcccatgc ccgccagacc agcagggggg
     1441 tggagacgac cactaccagg gtgacaatgg cgagtttctc ccacgaagtg ttctggaggc
     1501 tcccgaacat ccagaagacc actgcctgga gctgctcggt ggtgccggca tactgcagga
     1561 acgaggtcat ggcgccgaag aggtacatga gtatgatccc cgcgagcacc atgctctcag
     1621 cagagacccc ccggtaccgg gagaggccat agatcgcgac tgcggcaaaa gcgctcatca
     1681 tgaaggcaga gaccacgagg gcgatttcgc cggagagcgc ggtggggaca aagatgatgg
     1741 cgatcgccgc accaaacgtc gctgctgagg cgatgccgag ggtaaacggg cttgcgaggg
     1801 ggttacggag caccccctgc atcacggcac ccgcgatcgc gagcccgaat ccggccacaa
     1861 cagcgaagag gacccgatga aggcggagat cccacacgat ggtgaatgcg aggggatcgg
     1921 cagagaacga acccggaaga agcccggcca ggaccgccgt gaaaacttcc tcaaccgtca
     1981 gccccgcgga cccgatgatt gtcgagccaa ccgcgagcag gatgatgagc actgcgagga
     2041 ccccgacaaa gaggagcctc tttgcctgca tgcgcaggta ctggccggca atcttctctt
     2101 tctcgtcatc catgaccaca cactcctcac tttaccggga acacgaagcg gtcagcgcgt
     2161 gcaaggcccg ggtcgaggcc ctggaactcc tggagatacg tgcggtgcag tgattccggg
     2221 tccagatcgc ctgcgctgtc ggggtggaac cacctggcca tgtaagtcac gccgatcagg
     2281 tactgcggcc ctcccatgat cgcgttgtgg agcacgtaca ccctcccgtc ccggacggca
     2341 gggaggctgc tccacccagc ccttgggagg agggtgtccc tgacgtcttc aaagcgggtg
     2401 gtgttctccc ctgagtatcc cccgaagatg tacttcgagg agcccacgag cttgatcacg
     2461 acttcaggtt tccgggaaat gaccgcctcg ggatcgatct cgggatactc ggcagggttg
     2521 tcgcggaaga tgtttgctcc cccggccatc gtgaccttgt catggtagcc cgagcccctg
     2581 gcgacactct tgaaatcgtt ccaggtctcg aagtagacct cggggggtgc ggaggacgag
     2641 gcggccgcgt caaccttcgc gagggcaccg gcatagaacg ccgcgaggcg ggcaccttcc
     2701 tcctcctttc cgaaaatctc cgcgaggcgg gccgtatctt cgagataggt ttcgggacgg
     2761 tagcagtcaa tcctgaaaac agtgagatcg ggccttgaga gagtgaccct ccgctcgatc
     2821 tcgtcgcact ctgccttgct caccgatgca tacaggaaaa gggcatcagg acgaagcgag
     2881 agcatctttt cgtagtccgg ggcccagatt gatccgactg agggataccc ggcgtattcc
     2941 gggaaaaatt ccggtttctg cgcgatgtac ttgtccacgg ccacgatcct ctcttttgtg
     3001 accccgaggg accgcatcgt ctccagggtc tcactgttca tcacgataat tcggcgaagc
     3061 gggcgcgtga gggtgaggct ctttcctgag gagtcggtca ccgtcctcgc agtcccgccc
     3121 cagtggacaa atacccatgc ggagtcccgg aggtcggaaa ttggggcatc cgaaatattg
     3181 gtgtcctgtg agaggtaacc gaggatcgag gaggcgagct cctccctcgc gatgaccccg
     3241 ttatcgtcaa ggtcgccggg aagcgcggca tcggccagaa caatgggaca gaggaccgcg
     3301 aaaaccacga tcactgtcaa cgggaacctg ttcattcctc gtccctccaa cgagcccggc
     3361 atctcgccac acctatgaag gcaagcccgg cccctcccag gaggagaagg ggcgaagccc
     3421 ctgccttcgc ggtccccgtg gtcaccggaa ctgccggggg aacgccctgg ccctgtgtgc
     3481 cgtctgattc ccctccgact gtcccctcct cgccggaaag atagtctgtc cacgtccccg
     3541 tgatcgctcc cgctgggtcc tcttgtccat gcacgaagta ggtgatctcg cgttcaccga
     3601 tcaccgcaag aaagagtgtc gccccatcga ccctgtactg gtcagggggc agggagacct
     3661 cccgcacctc cattccggag gggagaacct caatgatccc ggcgacagtc tccccgggga
     3721 gagtcagcac gacgcggtag gtagaatgct ctgaaggctc gatggaccgc tctacaatgc
     3781 cggctgctgc cgcctggacc agcatggcgc agatgatcat gggaattatc ccggtgacaa
     3841 ggcgccctct cttcatatgg atagttcttt gactgtgtta cattaaataa taatggttat
     3901 aatttcgtct gtaccaggaa gagaaaatag caatatatca caaatttcga aatatagtag
     3961 cactaattga caaattattt tactaacttc aacaatcaac tcctctctct cctcctgtaa
     4021 tgacacaatt tacgacgggt cggaagtgct atcagcccag gaacggaaga gattattctg
     4081 agactctccc gcaagacacg agcgacgaaa tgatcccgat cgagacatcc gccctgacaa
     4141 aatactacgg atcactctgc gcagtggacc acctggacct ctccatcgga aacgagatct
     4201 tcgccctgct cggcccaaac ggctccggga agaccacgac agtgctgatg ctcaccacgc
     4261 tgctccggcc gaccgcgggg tctgcgcgta tctgcggcca cgatatcgag agcgatgcag
     4321 gggaagtacg aaggctcatc agttacgtgc cccaggacat ggccgtcgac atcaagctga
     4381 ccgggaggga gaacgtgaca ctctttgcaa agctctacgg gatatcccac cgggacgaga
     4441 ccattgaccg cgtgctcggg atgatggccc tctcggaacg agccgacgac ctggtccgga
     4501 cctactcggg agggatgcgg cggaggctcg aacttgccca ggcactcgtc cacgagccga
     4561 aagtcctctt ccttgacgag cccaccatcg ggctcgatgt cgcggcaagg aagaagatct
     4621 gggagcacat ccggaccctg aaatcccggg ggatgacgat ctttgtcacc acccactaca
     4681 tggacgaggc cgaccacttt tgtgaccgtg tcgcgattct cgatcacgga cgggtggctg
     4741 cagtcgacac tcccgcacgc ctgaagggga tgcttggaaa agacctcatc agtgcgaggg
     4801 tgagcggggg gttctcggga gatctccccg cggggatcga gcgcctgggc gaaaaggaga
     4861 acggggagta tctgttctca tctgaccatg catccgagaa ggtgccggtg ctcttccgct
     4921 ggctcgagga gcacggggca gtcatccact ccctctcggt acgtgagccg accctcgacg
     4981 acgtcttcct cgcgcttgtc gggtcaaggg acgacgtgac ggggttcgac gaccacaggt
     5041 tcaggacgat gctcaggaga agatgatgaa ggacatcctc tcatacttcg agcgggacat
     5101 gctccgctgg atacggggac gtgtctcggt catctccgcg cttgtcgtgc cggccgcctg
     5161 gctcgtcttt gtcggccttg ccctccccac cacgttcacc gatcactaca tcgacttcat
     5221 cactccaggg atcctggtcc tgacgatgct cggcgcctct ctccagggag gctccctcct
     5281 catcttcgac aagatcctcg ggttcctcaa caagttcctc gcactgccct cctcgcggga
     5341 gagcatcctc tacggaaaga tcctcttcat cacgacgcgg gggctgatac aggcaaccgt
     5401 aatcctcatc ctcgcgttca tcctcggcgc aaccttcctc tcaccctgga gcatcgtcct
     5461 cacgtatggc atcctggcag tgttcggggt gctcctctcg gcgttctcga caactgttgc
     5521 gctctacctt tccgaccacg acgcgtatgc cgcgttcaac gccatggtga gcatgccgct
     5581 cttcttcacc agcagcgcac tgatgccgta cgatgtcatg ccctcgtggc ttgcccccct
     5641 cgcctactgg aaccctgtca gctatgcgat tgacgcgatc agggcgctcc aggagggagt
     5701 cctcgcctgg gggaggatag gcgcccttgt cctcctcgcg gtgctggtcg ttgcgatcag
     5761 cgtccggcag ttccgaaaag tgacactgta atcaatacaa cgatccagtg gccagacgtg
     5821 aacgatcaaa attctgcggg accttaactc gcaactgtgc ataatttttt tataagaatt
     5881 tataaaaatc gcacatagtg ctacgaatat gcaaatattt attacattaa tctgcgtacc
     5941 actttactgg cagaatcagc catgtgattc tctgccgcag ggatcacccg gacttctcgt
     6001 ctcctcgatt tcctgctcac atcgcctcat ggaaaatgca cacataggag agaatgtcat
     6061 ggagatgacc gtgcacgaac agagtaattc cacactcgat accgatcggg acacctgcct
     6121 gttccgggct ccaagggacc cggaccgcat cgcgtatctg cggggtaaag atctctatga
     6181 acacagacgc aatgatcagc catccctcta ctacatccat cgctggtcgc ttgcacctgg
     6241 cgagagggaa actctctcgc tcatctctcc ggtcatggca ggccggttcc ttgaatcgaa
     6301 gggaatcttc tgccccgaga ccggagactt tcgcggagcg gcaatattgc ggtcctgggg
     6361 atatggaatc ctggaagaat tctgaaatac cgccgcctga ctgcgagagt ctatccatac
     6421 gccgccatca ccgtcccctc agcggggagc cgggccaccg gtcccccgga aggcggcgt
//
LOCUS       DAOQYR010000110         6419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_12458_length_6419_cov_6.074953, whole genome shotgun sequence.
ACCESSION   DAOQYR010000110 DAOQYR010000000
VERSION     DAOQYR010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12458_length_6419_cov_6.074953"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1090)
                     /locus_tag="PKM87_10385"
     CDS             complement(89..1090)
                     /locus_tag="PKM87_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNQ30515.1"
                     /translation="MPAEYRAYITKIGDLKQDIHDSLSFIGWENHVNKDDTVFVKPNF
                     TYPFFKEGITTNPAFLRSLLELLKDRASRVIVGESDGGNHSFTADQAFNGHGMPEICK
                     ETGAELVNLSTLPSRYIEDAIQGKKVKVQVPNLLVDGIDCFISVPVLKVHVMTRVTLS
                     MKNLWGCYPDTMRCLHHKYLSRKLTLLTRVLDPKIVLIDGTYALDGHGPMFGNAVHAD
                     LIIAANNPVVADAFGSAIMGIPVSQVEHIMIAEKDGLGTTNLETVQFNGDWTRYRMQF
                     EVNKTIIDRLSTILFNSEICAKCVMDSPMKPIIYGVATKLRNTDEKCVNDELKRGHG"
     gene            complement(1080..2270)
                     /locus_tag="PKM87_10390"
     CDS             complement(1080..2270)
                     /locus_tag="PKM87_10390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ30516.1"
                     /translation="MRILVVQESNWITKGPHQSHHLMERMVSRGHEVRVIDYDIVWRN
                     NPKRKILPPRLEVRTPPKVIPDAYITVIRPAIIQLPLLDYTTLLYSHRKEIIQQLDTF
                     KPDVVIGFGILNARIAISQCRARNIPFVYYIIDELHRLVPQPFFQGYARSIESENMKQ
                     SDLVLSINEGLREYTISMGAAPEKTRVIRAGIDFAWLNNADRDKKRKELGIVQEDIIL
                     FFMGWLYEFSGLREVASELMKQDPSSRLRLLAVGEGDLWDDLQAMRSAPGSGERIITI
                     GWQPYATIPDFLATADICILPAENNEIMRNIVPIKMYEYMAAGKPVIATELPGIMKEF
                     GSGNGVVYVENAGGVIRKATELSHNGSMIHLGAKARQFVIPNDWDMITDTFEQTLGGI
                     MDAC"
     gene            complement(2278..3276)
                     /locus_tag="PKM87_10395"
     CDS             complement(2278..3276)
                     /locus_tag="PKM87_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNQ30517.1"
                     /translation="MEHFEEFYQDSTILVTGGAGAIGGNLCRALSSLGAKKVIILDNL
                     ASSYEWNIPQAPNISFVQGDILDDESLKRVFKEKPDYVFHLAAHFANQNSVDNPETDL
                     MVNGMGILNVLQYAHMVGVKRFVYSSSGCGVYGLDSKMPFEEHDVSIRLHTPYQVTKL
                     LGELYTNYFYNLYHLPIVNARFFNSYGPGEVPGKYRNVIPNFFYWAMKGEPLPITGDG
                     TETRDWTYVGDIVNGLLAMGVREEAIGEAFNLGAGQEQRVIDMANSVLSLTGSKAGIV
                     YRERRDWDKKTHLLSCITKGQRMIGYQPRMTFDDGLKETHRWFVENWENIEKGAEF"
     gene            complement(3355..4707)
                     /locus_tag="PKM87_10400"
     CDS             complement(3355..4707)
                     /locus_tag="PKM87_10400"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HNQ30518.1"
                     /translation="MISGGENEQVEFKPSLSQKDKIMESVSAFSNTCGGTILIGVTDT
                     GNVTGLDVGRRTLEDLAGYIKRNSDPPVYPSLQTVQFGTRTLLSIQVKENPEKPVFYQ
                     DKAFRRVGRSNQRISSHEIRKMAKEEKKKVTWDERTCEGATLDDIDWAFVKDEFIPLY
                     ERISKKKVASSPLEFLKAARTIQNETPLNAGILLFGKDPLKFFPNSYIALARYAGTVV
                     GGEKRDYKEFRGNLFSQIDNCYAYLTEHTALMSRLSPGQLRRTDIPEYGSFSLRELVT
                     NAVCHRDYEDQGGKIIIKMFDDRIEFFNIGGLAQGITVDNIVHAQYSRNPVITSLLAK
                     VNYIEEMGEGWDKILKEHRDHPLMPQMPEILPGSHSVQVTIFSTREKFGEMQRDILND
                     RQRAIMAYLKEHGTITRTTCVDVLGVSKDTALRELTALLSRGLIQRQGVGRGIYYVLS
                     "
     gene            complement(4741..5679)
                     /locus_tag="PKM87_10405"
     CDS             complement(4741..5679)
                     /locus_tag="PKM87_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNQ30519.1"
                     /translation="MESKRILVTGGAGFIGTNLVNELKSHGYDVFAVDLYNTERDDYC
                     RVDIRNFRQIERVFEKNSFDYVYHLAAEYGRWNGEDYYENLWQTNVIGTKHMLRLQEK
                     RKFRMIFFSSAEVYGDYEGVMTEDVMEKNPIRDTYQMNDYAITKWAGELMCLNSAKMF
                     GTETVRVRPVNCYGPHEHYTPYRGFIPKFIYHALHDKPYTVFKGHKRIIDFVEDSCRT
                     WANIVDNFIPGEVYNVAGRPEWEREIKEYSDLILKAVGRDDSLVTYEEAEPFTTKVKT
                     IDCSKAIRDLDHNPRVPPEEGIRRTVDWMKHYYRTN"
     gene            complement(5689..>6419)
                     /locus_tag="PKM87_10410"
     CDS             complement(5689..>6419)
                     /locus_tag="PKM87_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ30520.1"
                     /translation="VLQHPINLGKAQAMFKGFARAREYNPLAIVMLDADGQHNPDEIP
                     ELLKPVLAGKADLVVGSRYLNNKNDIPAYRVLGQKTLDLATNAGTGTRSTDTQSGFRV
                     LGRRAMLNLDFYSEGYNIESDMLSHFAQRGLTIGEVPIGVSYSVTNGHKKNPISHGLD
                     VLSHVIGLVGYRRPLISFGVPGGVLTVIGVTSLLYTLTELYTGGAFHSVMFVGGISSL
                     ILGLLLVTTSLILNSLVQVMKIDK"
BASE COUNT         1403 a         1718 c         1633 g         1665 t
ORIGIN      
        1 cgcttcgcaa gttcttttga tagctcataa aggacagtca ccgatccgcc aaacttcggt
       61 gaaaaaaaag gaatcacctg caatattttc atccatgccc tctttttaat tcatcgttca
      121 cgcatttttc atcggtattc cgtaacttag ttgccacacc ataaatgatc ggtttcattg
      181 gggaatccat cacacatttt gcacatatct cactgttgaa aaggattgtt gagagccgat
      241 ctatgatggt tttattcacc tcgaattgca tccggtatct tgtccaatcg ccattgaatt
      301 gaaccgtctc aagattcgtc gtcccaagcc catccttctc tgcaatcatg atatgttcaa
      361 cctgtgaaac cggaatcccc ataattgctg aaccaaacgc atcggcaacg accggattgt
      421 tagccgcgat gatgagatca gcgtggactg cgttgccaaa catcgggcca tgaccatcaa
      481 gggcatacgt cccatcgatc aggacgatct tcggatcaag cacccttgtc agaagagtca
      541 gtttcctgct caaatacttg tggtgcaggc accgcatggt atcagggtaa cagccccaga
      601 ggttcttcat gctcagggtg acccgcgtca tcacgtgcac tttgaggaca ggcacggaga
      661 tgaaacagtc aatcccgtca acaagaagat tcggcacctg gactttcacc ttctttccct
      721 ggatggcgtc ctcgatgtat cgtgaaggga gggttgacag gttgacgagt tccgcaccgg
      781 tttctttgca tatttcgggc atcccgtgcc cattgaaggc ctggtctgcg gtaaaggaat
      841 ggttgcctcc atcggattcc cccacgatta cccgggaagc acggtctttc agtaattcta
      901 atagagatcg gaggaaagca ggattcgtgg tgatgccctc cttgaagaaa ggataggtga
      961 aattgggctt gacgaatacg gtatcatcct tgttcacatg gttttcccac ccgatgaagg
     1021 aaagactgtc gtggatgtcc tgcttcagat caccaatttt tgtgatatat gcacggtatt
     1081 cagcaggcat ccatgatccc tcccagggtc tgttcaaagg tatccgtgat catgtcccag
     1141 tcattcggta tcacgaactg tcgtgctttt gcgccgaggt gtatcattga tccgttgtga
     1201 gagagttcgg ttgcttttcg aatgacccct cctgcatttt caacgtacac aaccccgttg
     1261 ccggatccaa attctttcat tatccccggc agctctgtcg caatcacggg ttttcccgca
     1321 gccatgtatt cgtacatctt gatggggaca atatttcgca tgatctcatt attctcggcc
     1381 gggagaatgc agatgtccgc agtagcaagg aagtccggga ttgttgcata tggttgccat
     1441 ccgatggtga tgattctctc acctgatcca ggtgccgatc tcatcgcctg gagatcgtcc
     1501 cagaggtcgc cttcaccgac tgccaggagc ctgagccggc tggagggatc ttgcttcatg
     1561 agttcggatg cgacctctct cagccccgag aactcgtaga gccatcccat gaaaaagagg
     1621 atgatatcct cctggacgat cccgagttct tttcgtttct tatcgcggtc ggcattattc
     1681 aaccatgcaa aatcaattcc ggcccggatg accctcgtct tctcaggggc tgcacccatg
     1741 ctgattgtgt actctcgcaa tccctcgttg atcgagagga cgagatcgga ctgtttcatg
     1801 ttctcgctct caatagagcg tgcatacccc tggaagaagg gttgtggtac gagccggtgc
     1861 agttcatcga tgatatagta cacgaagggg atatttcgtg cacggcactg ggagatggca
     1921 attcttgcat tcaggatccc aaacccaatc accacatccg gtttgaaggt atctagctgt
     1981 tggatgattt ccttccggtg tgaatacagg agagtggtgt aatcgaggag agggagctgg
     2041 ataattgcag gccttatcac ggtgatatat gcatcaggga tcaccttggg gggtgtgcga
     2101 acctctaagc ggggaggaag gatcttccgc ttgggattat tacgccatac gatgtcataa
     2161 tcgatcaccc ggacctcatg accacgggaa accattcgct ccatgaggtg atggctctgg
     2221 tgtgggcctt ttgtaatcca gtttgactcc tggacgacga gaattctcat ggggttatca
     2281 aaattccgca cccttctcaa tattctccca gttttccacg aaccagcggt gagtctcctt
     2341 caacccatca tcaaacgtca tcctcggctg gtacccgatc atccgctgtc ccttggtgat
     2401 acaggagagc aggtgcgtct tcttgtccca gtcgcgccgt tcacggtata cgatcccggc
     2461 cttgctcccc gtgagcgaca gcacggagtt cgccatgtcg ataacccgct gctcctggcc
     2521 ggcaccgagg ttgaaggcct ccccgatagc ctcttccctg acccccatgg caagtaggcc
     2581 gttcacgata tccccgacgt acgtccaatc ccgcgtctct gtcccatctc cggtgatggg
     2641 taacggctct cccttcatgg cccaataaaa gaagttcggg atgacattcc tgtattttcc
     2701 tggcacttca cctgggccat acgagttgaa gaaacgggca ttcacgatgg ggagatgata
     2761 gaggttgtag aaataattgg tatataactc tccgaggagt ttggtgactt ggtagggggt
     2821 atgcaggcgg atcgagacat cgtgctcctc gaaaggcatc tttgagtcga ggccgtagac
     2881 cccgcaaccg gatgaggaat agacgaaccg cttgactccg accatgtggg catactggag
     2941 gacgttgagg atccccatgc cgttgaccat caggtctgtc tcgggattgt cgaccgagtt
     3001 ctggttggcg aagtgggcgg cgaggtgaaa gacgtagtcc ggcttttcct taaagacccg
     3061 cttcaatgat tcgtcatcga ggatgtcccc ttgcacgaac gagatattgg gtgcctgggg
     3121 gatgttccac tcgtaggagg aagcaaggtt gtcgaggatg atgacctttt ttgcaccaag
     3181 cgaggagagt gcccggcaga gatttccgcc gattgccccg gccccgccgg tcacgaggat
     3241 cgtggagtcc tggtagaatt cttcgaaatg ctccatggtt atatcacctt ctcctgtaat
     3301 ccccaggaac gacaggggca ttttgcgtcg catttgcgtc gcatttgcgt cgcattacga
     3361 gaggacataa tatattcccc tcccaactcc ctgtcgctgg atcaaacccc tggaaagtag
     3421 ggcggtcagt tcccgcagcg cagtatcctt ggagactccc aggacatcca cgcaggtggt
     3481 tctcgtgatg gtgccgtgtt ctttcagata tgccatgatt gcacgttgcc tgtcgttcag
     3541 gatgtcccgt tgcatctccc caaacttctc ccgggtggaa aatatcgtca cctgcacgct
     3601 gtgagaaccg ggcaatatct ctggcatctg tggcatgagt ggatggtccc ggtgctcttt
     3661 taggatcttg tcccaccctt ctcccatctc ttcaatataa ttcactttgg caagcagact
     3721 ggtgatgacg ggattgcggg aatactgtgc atgcacgata ttgtccaccg taattccctg
     3781 agcaagaccg ccaatgttga aaaactcaat cctgtcatcg aacatcttga tgatgatctt
     3841 gcctccctgg tcttcatagt cccggtggca caccgcgttc gtgaccagtt ctctcaatga
     3901 gaaactccca tattcaggga tatccgttct tcggagttga cctggagaga gccttgacat
     3961 taatgcagta tgctctgtca ggtatgcata acaattgtct atttgcgaaa agagattccc
     4021 gcggaactcc ttataatcac gcttctcccc tccgacgaca gtgcctgcat acctggccag
     4081 ggcgatgtag gaattgggga agaatttcag aggatctttg ccgaataata agatccctgc
     4141 attcagaggc gtttcgttct gtatcgtgcg tgccgctttc agaaattcaa gaggcgatga
     4201 agcaaccttc ttttttgaaa ttcgttcgta aagagggata aattcatcct tcacgaaagc
     4261 ccagtcgatg tcgtcaagag tggcgccttc gcaggtccgt tcgtcccatg tgaccttctt
     4321 cttctcttcc tttgccattt ttcgtatctc gtgcgaggat atcctctggt tgctcctccc
     4381 cacacgccga aacgccttgt cttgataaaa cactggtttt tccgggttct ccttcacctg
     4441 gatcgagagc agggtcctgg ttccgaattg gacagtctgc agggatggat acaccggggg
     4501 atcagagttg cgttttatat acccggcaag gtcttcaagg gttctcctgc ccacatcgag
     4561 tcctgtcaca ttccccgtat cggtgacacc gatcaggatg gtccccccac aggtgttgga
     4621 aaacgcagac acactctcca ttatcttatc cttctgcgaa agtgaaggtt tgaactccac
     4681 ctgttcgttc tctccacccg aaatcatcct gggaatatcc gtggcggtat tcattcccgt
     4741 tcagtttgtc ctgtaatagt gtttcatcca gtcaacggtc cttcggatcc cctcttccgg
     4801 gggaaccctt ggattgtggt ccaaatcccg gatggccttg gagcaatcga tggtcttcac
     4861 cttcgtggtg aaaggctctg cctcctcgta ggtcacgaga gagtcgtccc tgccaaccgc
     4921 cttgaggatc aggtcagagt attccttgat ctccctctcc cattccggtc tgcctgccac
     4981 gttgtagact tcgcctggga tgaaattatc gacgatattt gcccatgttc ggcaggagtc
     5041 ctcgacaaaa tcgatgatcc tcttgtgtcc cttgaacacg gtatacggtt tgtcatggag
     5101 tgcgtgatag atgaacttgg ggatgaaccc acggtagggg gtatagtgct cgtgggggcc
     5161 atagcaattc accggtcgga cccggacagt ctctgtcccg aacatctttg ccgagttcag
     5221 gcacatgagt tctcctgccc acttcgtaat ggcataatcg ttcatctggt aggtgtcgcg
     5281 gatgggattc ttctccatca cgtcctcggt catcactccc tcgtaatcac cgtagacttc
     5341 cgcactggag aagaagatca tccggaactt ccgcttttcc tggagacgga gcatgtgttt
     5401 cgtgccaatc acgttggtct gccagaggtt ctcgtaatag tcttcaccgt tccaccgtcc
     5461 atattcggct gcgaggtgat agacgtaatc aaaggagttc ttctcgaaaa cacgctcgat
     5521 ctggcgaaaa ttccttatat cgacgcggca ataatcatcc cgctctgtat tatagagatc
     5581 gacggcaaag acatcatacc catgggattt cagttcattt acaagattgg tcccaataaa
     5641 tcctgctccg cccgtgacaa ggattctctt actctccatg aaaagaagtt atttgtctat
     5701 tttcatcact tgcaccaggc tattcaaaat caagctcgtc gtcaccagca gcaaccctaa
     5761 gattagcgag ctaatcccgc cgacaaacat caccgagtga aacgctcctc cggtatataa
     5821 ctcagtcagg gtatacaaca acgaagtaac cccaatcacc gtcaacaccc cgcccggcac
     5881 accgaacgag atcagcggcc tccggtaccc gacaagcccg atgacgtgac taagcacgtc
     5941 aagtccatgc gagatcgggt tcttcttgtg gccgttggtg accgagtagc tcacgccgat
     6001 gggcacttcc ccgatcgtca agccccgctg tgcgaagtgc gagagcatgt cggactcgat
     6061 gttgtagcct tccgagtaga agtcgaggtt cagcatcgca cgccgcccga gcacgcggaa
     6121 ccctgattgc gtatctgtgc tcctggtccc ggtgccggcg ttggtcgcga ggtcgagggt
     6181 cttctggccg aggacgcggt aggcggggat gtcgttctta ttgttgaggt accgggagcc
     6241 gacgacgagg tcggcctttc cggccagaac gggcttgagg agttcgggga tctcgtcggg
     6301 gttgtgctgg ccgtcggcgt cgagcatgac gatggcgagg gggttgtatt ccctggctct
     6361 tgcgaacccc ttgaacatgg cctgggcttt cccgaggttg atggggtgct gcaatacct
//
LOCUS       DAOQYR010000111         6070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_13302_length_6070_cov_6.015628, whole genome shotgun sequence.
ACCESSION   DAOQYR010000111 DAOQYR010000000
VERSION     DAOQYR010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13302_length_6070_cov_6.015628"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="PKM87_10415"
     CDS             <1..152
                     /locus_tag="PKM87_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNQ30521.1"
                     /translation="FEKGYGKGTGLGLFLSREILSITGMTIRETGIPGEGARFEIVVP
                     KGMWR"
     gene            complement(531..824)
                     /locus_tag="PKM87_10420"
     CDS             complement(531..824)
                     /locus_tag="PKM87_10420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HNQ30522.1"
                     /translation="MIIAEISIVPVGTGTPGVSTYVKAVLEVLKKQDRVRVDPGAMGT
                     VIEASNLQDLFDAVEKAHNSVLEMGAKRVVTEIKIDERRDKEATIESKTRALQ"
     gene            1229..1618
                     /locus_tag="PKM87_10425"
     CDS             1229..1618
                     /locus_tag="PKM87_10425"
                     /inference="COORDINATES: protein motif:HMM:NF039183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HNQ30523.1"
                     /translation="MNAVAVPKADLVGSFYRSQIPNNVFLCIILIYVLFVLDIVTTDM
                     ILSLGGFEVNHVMVPVVDNVFFHLLIKGFVLIFIVSVAQWSELKLKGSGLIMMLVITG
                     WYSFVVMNNTSVLIHLCEERCPRSPFF"
     gene            complement(2243..3586)
                     /gene="cobB"
                     /locus_tag="PKM87_10430"
     CDS             complement(2243..3586)
                     /gene="cobB"
                     /locus_tag="PKM87_10430"
                     /EC_number="6.3.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenobyrinic acid a,c-diamide synthase
                     (glutamine-hydrolyzing)"
                     /protein_id="HNQ30524.1"
                     /translation="MRITIPRIVIAGTHSGCGKTTVASGLMAALNDLGFRVQPFKTGP
                     DFIDPSHHSLLCGRSSRNLDPFMMGEAGVLRTFASASKDVDISVIEGVMGLFDGIDDT
                     DIASTAHVARILRAPVILVVDAYAASRSALAMVRGFCDFDPRIRIVGVIFNRIASPRH
                     RKMIGNRGPVPALGWIPYRKEDEVGGRHLGLVMAHECGMMKAYGQVVQESCDIDRILG
                     AARNAPSLDIPQIPPETIERETRAVIGVARDPAFCFYYQDNIDRLARAGAGIRFFSPM
                     DDLLPDMDAIYLGGGYPELYARELEVSRCRHSIHKMAKSGMPIYGECGGLMYLSHSIS
                     TDQTYRMAGVLPACAEITQKIQALGYVKGRYSGRSGSWTGKISLRGHEFHYSRVECDP
                     DARFAIRLMTGIGITGGYDGLTEGGAIGAYTHAYFSDAFCRRFVAAALEFRNRGG"
     gene            complement(3702..4733)
                     /locus_tag="PKM87_10435"
     CDS             complement(3702..4733)
                     /locus_tag="PKM87_10435"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNQ30525.1"
                     /translation="MNPARIQNHHTLVILVMMLVTMVLPASAVTVTDDAGVTISLDAP
                     PLRIVSLAPSNTEILAALGLVDRMVGVTDVCDYPPEAKQIPRIGGYSAISVEKVAAAM
                     PDLVLASDITPKATVDRLKGLGLTVMVVSPRNIDDMMRDIRVVGTITGKESEAEELVT
                     RLSKRIAAVARCSPDADHPTVAHVVWHDPLYVSGNDTLQNDVIIHAGGENVFSDREGW
                     STVPLEEFLLKNPDILIVSGGGGMDSSEKDVILEVFMTSPQYASLSAVRNNRVYAVNA
                     DIISRPAPRIVEATEQVARHLHPECFNGTAAIPGDTAVIPVKSPGFCTGSALLLVSLV
                     FILHWKRNE"
     gene            complement(4734..5480)
                     /locus_tag="PKM87_10440"
     CDS             complement(4734..5480)
                     /locus_tag="PKM87_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNQ30526.1"
                     /translation="MKKPLFISTYCCIDHSLDTALETLATRTSHVEILSDGLHDLRRE
                     SGTCNDHSLSYSVHAPSSEMNIAAVSERIRTASLEDLGDVMAICNRIGAKHLVVHPGY
                     SPYVQMWNRSYSSLLHSLDDLVRLQDEYGVLACVENMGAWECCHFRTPSFLPELTFRG
                     LHCTLDCGHARLNGNLDEFLAAGDFCHIHLHNNDGENDDHGACSEGTIDYHRLWNKLP
                     RGATLVVETRELASADQSLRYLSTLNQGEH"
     gene            complement(5477..>6070)
                     /locus_tag="PKM87_10445"
     CDS             complement(5477..>6070)
                     /locus_tag="PKM87_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30527.1"
                     /translation="REVYGADVVTSVHPVTGRTFLMPVSAPVRTIAPDLKRNIMVISG
                     GGSGSDLLHFLTRRGYPVAAGILATTDTDYLTVKSLEIPCEEVPPFSKIPEHSLTTLR
                     ESLNQAERIILSMHPVGPGNISVLTMLREVDPARIIIHLPEGRDISSYDFTLGAASAV
                     IQDLCDAGALCTGTFNGILKLLSTPPGAPGSTRSMQQ"
BASE COUNT         1353 a         1630 c         1618 g         1469 t
ORIGIN      
        1 tattcgagaa ggggtatggg aaagggaccg gccttgggct ctttctctcc cgcgagatcc
       61 tctccatcac ggggatgact ataagggaga cgggaatccc cggggaagga gcgcgtttcg
      121 agatcgtcgt cccaaaaggg atgtggagat agaagggaag cgtgtggtga tcatggtacg
      181 atcacccttt taatgcgaaa acaagaatga cgcaatttgc agcaggtcca acgcatgaaa
      241 atgccggagg cttttcatag gaaatcacat ccgggatttc attttttctg cacgtgtccc
      301 gacagtgaat catgcaattt tctctaaaaa cagtctgcac aacatttccc tgtcaatttc
      361 aagtgcagct catgccaggc cggcccttga ggcaggcgcc gccacataaa gatatcattg
      421 aatcggacgt tcccatgacc tggcggatgg ccacgagttt ttttaaaaag agatctcatc
      481 tccaggacaa tgccatgaat atacagctta tgtgatgaaa gggaggtggt ttattgcagg
      541 gccctggtct tcgactcgat cgttgcctcc ttgtccctcc tctcgtcaat ttttatctct
      601 gtcacgaccc tttttgcgcc catctcgagg actgaattgt gcgctttttc gaccgcgtca
      661 aacaggtctt gcaggttcga agcctcgatc actgtcccca tggccccggg gtcgaccctg
      721 acccggtcct gttttttcag tacttcgagc actgctttca cgtacgtgct cacaccgggg
      781 gttccggtcc ccaccgggac tatgcttatt tctgcgatga tcatgggttc tctccccaca
      841 tgtatccttc aaagaagcct gcgaattcta tgctatagct tcttttgatc tccacacacg
      901 catctttgcc agtccgggaa gatagcgttt ctgtcattgc tggttatttt ttatctgtca
      961 tttttttcgc aagggacacc tccctttcaa atcccggtct tcctttaatt cccgaggaga
     1021 gactcataag gtcttgtaaa aaaaatggcg agcccaaggt tcatggcatt gatccatgtg
     1081 aaacgggaaa gtgaaccgtt caagttatgc tcatgctcgt aaagtgactg gacgtgaaga
     1141 tttaataggt cccaaaattg atcatttcga aggctatgag acgatgcgtt ggatggactt
     1201 cccgggacat tgcaggaggg gttttcccat gaatgccgtc gcagtgccga aagcggacct
     1261 ggtcggatcg ttctacagga gccagatccc aaacaacgtc ttcctctgca tcatcctcat
     1321 ctatgtcctc tttgtcctcg acattgtgac caccgacatg atcctctcac ttggaggttt
     1381 cgaggtgaac cacgtgatgg tccccgtagt ggacaatgtg ttctttcacc tgctcatcaa
     1441 gggatttgtc ctgatcttca tcgtaagcgt ggcacaatgg tccgaactga agttgaaggg
     1501 gtcaggactc atcatgatgc tcgtcatcac gggatggtat tccttcgtgg taatgaacaa
     1561 cacgtcggtg ctcatccacc tttgtgaaga gagatgcccg cgctctccct ttttctgatc
     1621 cggacaggcg ggttattcct cccactcata ccagggaacg tcctgttaga cgcataagat
     1681 gagagtgagg aagcaaaatc gtgaagagtt cggaacttta caggctagaa gagtatagcg
     1741 ctccggatat tttccttgtg cgggagttcc ctggattttg tattgatctc atggtctttt
     1801 ctttccgtca gaacaattac agcctgccag gtggtttcac atccttttat ttccctctcc
     1861 agtctcacga gacgatgact attcaatggt atattccccc ggaactgggt tttcctcccc
     1921 tgggcaaccg gaggaacgtc agagatatgc tcccgttttc ccggtttggc atttcccgag
     1981 atacccgata ttctgggtta agtttttcat ctgcggcatc ttgagcatca gcgggttatc
     2041 actgtgatgc attgatagaa tttcctggag atcaaggatg catttgctga gattactatg
     2101 tgctcatgtg cattaccatc ctttttgggg aaggataaca tacatagccc tcatcgtggt
     2161 aggaactcca ttagtatgcc gttgaggggt atgcagcagg aggcataatc tgggcagaga
     2221 atctatttgc atatctcctt catcaacccc ccctgttccg aaattccaaa gccgcagcga
     2281 caaaccgccg gcagaatgca tcgctgaagt atgcatgggt atacgccccg atcgctcccc
     2341 cttcggtgag cccgtcgtat ccccctgtta tcccaattcc cgtcatcagg cgaatcgcga
     2401 accttgcatc gggatcgcac tcgacccggg aatagtggaa ttcatggccc cgaagggata
     2461 tcttccccgt ccatgaaccg gaccggccgg aataccttcc tttcacgtat cccagcgcct
     2521 gaatcttttg agttatctct gcacaggctg gcaatactcc tgccatccgg tacgtctggt
     2581 cagttgagat tgagtgagac aggtacatga gcccaccgca ttcaccatat atcggcattc
     2641 ctgatttcgc cattttgtga atggaatgcc tacatcgcga aacttcgagt tccctggcat
     2701 acaattccgg gtatccaccg ccgagataga tggcatccat atccggaagg aggtcatcca
     2761 tcggtgagaa aaaccgaatc ccggcccccg ccctggcgag ccggtcgata ttatcctggt
     2821 aataaaagca gaatgcagga tcgcgggcaa ccccgataac tgcacgcgtt tctctctcaa
     2881 tcgtttctgg agggatttgg gggatgtcca gggaaggagc attccgggct gctcccagga
     2941 ttcgatcgat atcgcaggat tcctggacaa cctgcccata ggctttcatc attccgcact
     3001 catgagccat cacgagtccg aggtgacgcc cgcccacttc atcttccttc cgatagggaa
     3061 tccatccgag cgctggaaca ggtccccgat ttccaatcat tttccgatgc ctgggtgatg
     3121 ctattcggtt gaatattacc cccacaatcc ggatacgggg atcgaaatca caaaatcccc
     3181 tgaccatcgc aagggcgctc cttgatgcag cgtacgcgtc tacgacgagg ataaccggtg
     3241 cccggaggat cctggcgacg tgagcagtgc tggcgatgtc ggtgtcgtcg attccgtcaa
     3301 agagccccat aacgccctcg atcacagaga tatccacatc tttagacgca gaggcaaacg
     3361 tccggaggac gcctgcttct cccatcatga aagggtcgag gttgcgcgaa ctcctcccgc
     3421 acagaagcga gtggtgggaa ggatcgatga agtccggtcc tgtcttgaat ggctggaccc
     3481 ggaaaccaag gtcattgagc gctgccatca gaccgcttgc aacagtggtc tttccgcagc
     3541 cgctgtgagt gcctgcaatt acaatcctgg gaattgttat ccgcatgcaa ccctcctggc
     3601 tccgtatctg ctgccgggac tgccccctgt tgctggcaga tgtatttcct cctggcagcc
     3661 gggaaggagc tgaatggacc tttcttctgt tcttaagtct ctcattcgtt cctcttccaa
     3721 tggaggatga ataccaggga gaccagcagg agtgcactcc ctgtgcagaa tcccggggat
     3781 ttcacgggga ttaccgctgt atcgccagga atggcggccg ttccattaaa acactcggga
     3841 tggagatgtc gggcgacctg ctcagtggct tccacgatcc gcggggctgg cctgctgatg
     3901 atgtcggcat tcactgcgta gacccggttg ttcctgactg cggagaggga tgcatactga
     3961 gggcttgtca tgaagacctc aaggatcacg tctttttcag atgaatccat acccccaccc
     4021 ccgctcacaa tgaggatatc agggttcttt aagagaaatt cttccagcgg gacagtgctc
     4081 cacccttccc ggtcagaaaa gacgttttcg ccacctgcat ggataatcac gtcattctgg
     4141 agagtatcgt tgccgctcac atagagcggg tcgtgccaga caacgtgtgc gacagtcggg
     4201 tgatccgcat cgggcgaaca ccgtgccacc gcagcaatac gttttgagag gcgtgttacc
     4261 aattcctctg cttcgctctc ctttccggtg atcgtcccga ccacgcggat atcccgcatc
     4321 atgtcgtcaa tgtttcgcgg agagaccacc ataacggtca gaccgagacc cttaagccga
     4381 tcgacagtcg cctttggggt gatatcggat gcaagaacaa ggtccggcat tgcggcggcc
     4441 accttctcga cactgatcgc cgaataaccc ccgatgcggg ggatctgttt tgcttccggg
     4501 ggataatcgc agacatcggt cacacccacc atcctgtcga cgaggccgag cgctgcaagg
     4561 atctcggtat tggagggggc gagcgagacg atgcgcagag gaggcgcatc aagggagatg
     4621 gtcaccccgg catcatcggt caccgtgact gcagatgctg gcagaaccat tgtgaccagc
     4681 atcatgacaa ggataaccag ggtatggtgg ttctggattc gggcaggatt catttaatgt
     4741 tcaccttggt tcagggttga gaggtatcga agactctggt cggcactggc gagttcccgg
     4801 gtctcgacga caagggttgc accccgcgga agtttgttcc agagtctatg gtaatcgatg
     4861 gttccttcgc tgcatgctcc atggtcatcg ttctctccat cattattgtg gagatggata
     4921 tggcagaaat ccccggctgc aaggaattca tcgaggttcc cattcagcct ggcatggccg
     4981 caatcaagcg tgcagtgaag gccgcggaag gtaagttcgg gcaggaacga aggcgtgcgg
     5041 aaatggcagc attcccaggc gcccatgttc tccacgcagg ccaaaacgcc gtattcatcc
     5101 tgaaggcgga ccaggtcatc gagcgaatgc aggagagagg aatacgagcg gttccacatc
     5161 tgaacgtagg gtgaataccc cgggtggacg acaaggtgtt tggcacctat ccggttgcag
     5221 attgccatca catcacccag atcctccagg gatgcggtcc gtatccgctc gctcacggcg
     5281 gcaatgttca tttcacttga cggtgcgtgg acagaatacg acaacgagtg atcgttgcat
     5341 gttccactct cacgacggag atcatgcagc ccgtccgaga ggatctccac gtgcgatgtc
     5401 ctggttgcca gggtctcgag cgccgtatcg agcgaatggt cgatgcagca atacgtcgag
     5461 atgaaaagag gtttcttcat tgttgcatac tccgtgttga accgggtgct ccaggtggtg
     5521 tggacaggag tttgagtatt ccattgaagg tgccggtaca gagcgcaccg gcgtcgcaca
     5581 ggtcctggat caccgcagat gcagcaccca gggtaaaatc atacgaggaa atatcccggc
     5641 cttccggaag atggatgatg atccgggccg ggtcaacctc ccgaagcatg gtgagcacgg
     5701 atatgttgcc aggtcccacc gggtgcatcg agaggatgat ccgctccgcc tggttgaggc
     5761 tctcccgcaa tgtggtgagt gaatgttcag gtatttttga gaatggcggg acttcttcac
     5821 aggggatctc aagcgacttc accgtgagat aatcggtatc cgtggtcgca aggatcccgg
     5881 cagcaacggg atatcccctg cgggtgagaa agtgcaggag atctgatccg cttccccctc
     5941 ctgaaatcac catgatgttc cgcttcaagt ccggagcgat tgtcctgacc ggtgcggaaa
     6001 cgggcataag gaaggtccgg ccggtcaccg ggtgcacgga cgtgaccaca tcagccccgt
     6061 agacctcccg
//
LOCUS       DAOQYR010000112         6048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_13363_length_6048_cov_9.270649, whole genome shotgun sequence.
ACCESSION   DAOQYR010000112 DAOQYR010000000
VERSION     DAOQYR010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13363_length_6048_cov_9.270649"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /gene="surE"
                     /locus_tag="PKM87_10450"
     CDS             <1..689
                     /gene="surE"
                     /locus_tag="PKM87_10450"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNQ30528.1"
                     /translation="TQQSAVGRSISIFEPIRAHEVEMNGVRAWAVGGKPTDSVIIGLY
                     ALDLQPAMVVSGINIGENLSYESIMTSGTVGAALEAANQGTPAIAFSLQVEDQGDKFD
                     DPRHHAQSFEESKRVVKEVCGMFLDRRLPPGADILNVNIPSVVKGGYEVTRLGEKLFS
                     TGVEKRLDPRGRPYFWINGPLIADAGEGTDVHAIRKGNISLTPITLDCTAQGAGLALR
                     SLFLGAGGSI"
     gene            686..1399
                     /gene="rpiA"
                     /locus_tag="PKM87_10455"
     CDS             686..1399
                     /gene="rpiA"
                     /locus_tag="PKM87_10455"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HNQ30529.1"
                     /translation="MSTLPAERAKRDAGYHAAGMVEDGMVIGLGTGSTTYHAMVRLAE
                     RVAEKEITIAGVPTSHQTAIRARELGIPLTSLDDHPCLDLAIDGADQADPLLRLVKGR
                     GAALTREKVVACAAGRLVIVADPGKLVQALSGIVPVEVIPFAAVPVMHALADMGGAPR
                     IREGKAKDGPVITDNGNYILDCRFEEIPDPARLEDMLTLIPGVVECGLFCRYTEKTTL
                     VIGEERGCRVIRGGRDQEI"
     gene            complement(1387..1761)
                     /locus_tag="PKM87_10460"
     CDS             complement(1387..1761)
                     /locus_tag="PKM87_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNQ30530.1"
                     /translation="MKSRNVMYFTPREEEFADLLVRIGLKKNVAKVLVYLAHTPEATS
                     RDIERGTDLRQPEVSIAMATMMDQRWVENRETKAENKGRPVKIYRLSQPVGEIMDTIE
                     KEKRAEATNQLNLIQQLRDYIS"
     gene            complement(1870..2430)
                     /locus_tag="PKM87_10465"
     CDS             complement(1870..2430)
                     /locus_tag="PKM87_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HNQ30531.1"
                     /translation="MKVKPEESLKIENIVASAKVTDYLDLPALASQIKDAEYNKKRFP
                     GVVIRMQNPKIAALVFGSGKVVLTGAKSIESLSQGLEILGNLLRGLQIDIPKKLSYKI
                     QNIVTSADLGSGINLNKIAVGFNLDRIEYEPEQFPGLVYRLENPKVVVLLFGSGKLII
                     TGGKEPEDARKAVQKILTDLKNLGLL"
     gene            2644..3510
                     /locus_tag="PKM87_10470"
     CDS             2644..3510
                     /locus_tag="PKM87_10470"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNQ30532.1"
                     /translation="MQNRKDAGVNEVIASILMVALVIVLAIIIGSIVFGVIVIQPKSA
                     YIPPRVEVVNYSGQQAISLYSRGGDPAVLDKGDGVQYALGIYVENTSGSSRATPDPGV
                     KSFGPGQTLYIYYRNGEYRATDNLTGKTFDKLPPGPLTLRLVDENARLLVYKEGIGMG
                     GEGTVTVSPTPTPSCGTITGIVFNDLNGNGQRDPGEPGLSGWTVQYRYSKNLGQGSGS
                     GDWSAWISGTTGSDGAYTFSSLLYQPTHFYEVRVLLQQGYTTIKPQLVYSFKLTPNEN
                     ECYATGKDFGIS"
     gene            complement(3839..4492)
                     /locus_tag="PKM87_10475"
     CDS             complement(3839..4492)
                     /locus_tag="PKM87_10475"
                     /inference="COORDINATES: protein motif:HMM:NF039641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6064 family protein"
                     /protein_id="HNQ30533.1"
                     /translation="MDASYFFTQFAAYNTDWLPVNVILWLLLLITTALVFMRPADMRS
                     QALIKGALALVFLWNGIVFFFFYMTLSAVPGGIPMVVIGLLFATDIFRKRITISLPAS
                     GWVRYATLAWAIWALGLYTIAGWLTGHPYPGGPLPAAPCPTTILAIALLSTSMATLKT
                     TRLLFTLLFALLLWWAFFAGTFAPAMYGFYLDLTLLAAGVYGLVMILKNRPNAGVMG"
     gene            complement(4707..5018)
                     /locus_tag="PKM87_10480"
     CDS             complement(4707..5018)
                     /locus_tag="PKM87_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor S"
                     /protein_id="HNQ30534.1"
                     /translation="MFCPECRSLMISSAGQLKCRKCGYIRNIGKDDQMQIRKERRERE
                     IVIVEEEGSVRTLPTIATKCPKCEHNLAFWWLRQLRAADESEVRFFRCCECGHTWREY
                     D"
     gene            5156..5962
                     /gene="artA"
                     /locus_tag="PKM87_10485"
     CDS             5156..5962
                     /gene="artA"
                     /locus_tag="PKM87_10485"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase A"
                     /protein_id="HNQ30535.1"
                     /translation="MKELLVLFSCLFFLAFLVPWKLRNWCAVFGWTCIVLFLFAEVPY
                     YFSINNFLYPVMAVLSVPFLVITARHLITREVLVMQLSTAAAVAFLIYAPFAYSPLGD
                     WLISVVVGQTAWMLEAFGYPPLQLAWNMLSRNNFRVEIILACTGIQAIAIMLGVVSAV
                     KTNPRQKMLSFLLIVPTIYILNIVRNTAVIIAYTEQWFPFLPEIAGNGEFGYESFFWA
                     HNVLAEGLALLLLIGIAWGLFRLIPDLADFATGLVQLYWRDIRRAFGKDR"
     gene            complement(5934..>6048)
                     /locus_tag="PKM87_10490"
     CDS             complement(5934..>6048)
                     /locus_tag="PKM87_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNQ30536.1"
                     /translation="LLAGGLTGFIWLSVLVIAICTNLSALQRIIYLYQTLS"
BASE COUNT         1331 a         1656 c         1782 g         1279 t
ORIGIN      
        1 cgacccagca gagcgcggtg gggagatcca tctccatctt cgagccgatc agggcgcacg
       61 aggtggagat gaacggcgtg cgagcatggg cagtgggagg aaagcccacg gactcggtga
      121 tcatcgggct ctatgcgctc gaccttcagc cggcaatggt ggtgagcggc atcaacatcg
      181 gcgagaacct ctcgtatgag tccatcatga catcagggac cgtgggagcg gcgctcgagg
      241 cggcgaacca ggggacccct gcgatcgcct tctctctcca ggtagaggac cagggggaca
      301 agttcgatga cccgcgccac catgcgcaga gtttcgagga atcaaaacgc gtcgtgaaag
      361 aggtgtgcgg catgttcctg gaccggcggc tgccacccgg ggcagacatc ctcaacgtga
      421 acatcccttc tgtcgtgaag ggcggttacg aggtgacacg ccttggggag aagctcttct
      481 cgaccggagt ggagaagagg ctcgatccca gggggaggcc gtacttctgg atcaacggac
      541 ccctcatcgc tgacgccggg gaggggacgg atgtccatgc catccggaag gggaacatct
      601 ccctcacccc catcaccctt gactgtacgg cccagggtgc ggggcttgcg ctccgctcgc
      661 tcttcttggg ggcaggaggg agtatatgag cacgcttccg gccgagaggg ccaagagaga
      721 tgcagggtac catgccgcag gcatggtgga ggacgggatg gtcatcgggc ttggaacggg
      781 atcgacgaca taccatgcca tggtccggct cgcggagaga gtcgcggaaa aggagatcac
      841 cattgccggg gttcccacct cgcaccagac cgctatccgg gcacgcgaac tcggcatccc
      901 cctgacatcc ctcgacgacc acccgtgcct cgacctggct attgacggcg cagaccaggc
      961 cgacccgctt ctccgcctgg tcaaggggcg gggtgcggca ctcacgaggg agaaggtggt
     1021 ggcatgtgcg gcagggcggc tggtcatcgt tgcggaccct ggaaagctcg tgcaggcgct
     1081 ctccggcatc gtcccagtcg aggtgatccc gtttgccgca gttccggtga tgcatgccct
     1141 cgcggatatg ggaggagccc cgcgaatccg ggagggaaag gcaaaggatg gcccggtcat
     1201 cacggacaac gggaattaca tcctggactg ccgctttgaa gagatcccgg accctgcgcg
     1261 cctcgaggac atgctcacgc tcattccggg agttgtggaa tgcgggctct tctgccgcta
     1321 cacggaaaag accacgcttg tgatcggaga agagaggggc tgcagggtaa tcagaggggg
     1381 acgggatcag gagatatagt cccgcagctg ctggatcagg ttcagctggt tggtggcctc
     1441 agccctcttc tccttctcga tggtgtccat gatctcgccg acaggctgcg agagcctgta
     1501 aatcttcacc ggcctgccct tgttctcagc cttggtctcc ctgttctcca cccagcgctg
     1561 gtccatcatc gtggccatgg cgatgctcac ttcaggctgg cggaggtcgg tgccccgctc
     1621 gatatccctc gaggttgcct cgggggtgtg ggccaggtat acaaggactt tcgcgacgtt
     1681 tttcttcaat cctatccgca ccaggagatc ggcaaattcc tcctccctgg gggtgaagta
     1741 catgacgttt ctcgatttca tggtgttcac cgactcttct tatgttatta acatatatta
     1801 tataaaaaga ttattattat ttatatagca ttaaaaaaag gaaacgggag aaaaacaaaa
     1861 aaaatggtat cagaggagac cgaggttctt gaggtccgtg aggatcttct gcacggcctt
     1921 cctcgcgtcc tcgggctcct tgccgccggt gatgatcagc ttgccggacc cgaagaggag
     1981 gaccacgacc ttcgggttct cgagccggta tacaaggccg gggaactgct cgggctcata
     2041 ctcgatccgg tcaaggttga acccgacggc gatcttgttc aggttgatgc ctgagccgag
     2101 gtccgcggaa gtgacgatgt tctggatctt atagctcagt ttctttggga tgtcgatctg
     2161 cagcccgcgg aggaggtttc caaggatctc cagcccctgc gagaggctct cgatgctctt
     2221 cgccccggtc agcaccacct tcccggaacc aaagaccagc gcggcgatct tcgggttctg
     2281 catcctgatg acgactccgg gaaaccgctt cttattgtat tcagcatctt tgatctggga
     2341 ggccagggcg ggaagatcca ggtaatcggt caccttggct gaggcaacga tattttcaat
     2401 cttgagagat tcctctggct tgaccttcat gtttataaaa tgcccccgga cagtatataa
     2461 acagttgggt gcgaccccca gggtcacggg tcgatcagga acgccgcgat cgagaggaag
     2521 atcaggactc ccatgatgtc cgtgatcgaa gtgatcggct ggctgcacgg gtatggcaaa
     2581 acccaggaaa ccgggtgagt gacccatgga cggttttatc acctttctcc cgcaggtcta
     2641 agtatgcaga accggaagga tgccggtgta aacgaggtga tcgcatccat cctcatggtg
     2701 gcgctcgtca tcgtcctggc catcatcatc gggagcatcg tgttcggcgt catcgtgatc
     2761 caaccgaagt cggcctatat cccgccaagg gtcgaggtgg tgaactacag cggccagcag
     2821 gccatcagcc tctactcgcg gggtggggac cctgcggtcc ttgacaaggg agatggcgtg
     2881 cagtacgcgc ttggcatcta cgtcgagaac acgagcggat catcgcgtgc cacaccagat
     2941 ccgggggtca agtcgtttgg cccaggccag acactgtata tctattaccg caatggggaa
     3001 taccgtgcaa cagacaacct gaccggaaag acgttcgata agctcccccc tggtccgctt
     3061 accctgcgcc tagtggacga gaatgcgagg ctgcttgtgt ataaggaggg gattgggatg
     3121 ggaggagaag ggactgtaac ggtgagtccg actcctacac cctcatgtgg cacaattacc
     3181 ggtattgtgt tcaatgattt gaatggaaat ggccagagag accctggaga acccgggtta
     3241 agcggatgga ctgttcagta tcggtacagt aaaaacctag gccaaggctc gggatcgggc
     3301 gactggtcgg catggataag tggcacgacc ggatcagatg gagcatatac cttctccagt
     3361 ttactctatc aaccaactca tttttatgaa gtgagagtgc ttttacaaca gggttataca
     3421 acaattaagc ctcaattagt gtattcgttt aaactgactc caaatgaaaa tgaatgttac
     3481 gcaaccggga aagacttcgg tatcagttaa aattcacctc tataaaaaat ttcatccttt
     3541 ttacctgaag agtttcagga gaaagacaat aattcccaca ggagggaggg ggagaatccc
     3601 cctcccctgt cccctccccc aaaacgcgat agtccgcatg gaatagtagc atatctgaat
     3661 agaaaggact gtagattttt gtgatattgt ctatcagtat gctcattttt attccataat
     3721 ggaagcgatt ttacaatggg gtcggtacct gacagattcc tcatctgatc aggtaaaaca
     3781 acgggtaggg aggactacca atgcagaaaa gaaaaagtgc catcctcctc ccttcacatc
     3841 atcccataac acctgcattg ggccggttct tgaggatcat gactagtcca tacacgccag
     3901 cggccagcag cgtaaggtcc aggtaaaagc cgtacatcgc aggggcgaag gtgccggcaa
     3961 agaacgccca ccagaggagg agagcgaaca ggagtgtgaa gagaagacgg gtcgtcttca
     4021 gcgtcgccat cgatgtcgag aggagggcga ttgccaggat cgtggtggga catggtgcgg
     4081 caggaagcgg tcccccgggg tagggatggc cggtcagcca tccggcgatc gtatagagtc
     4141 cgagcgccca gatggcccag gcgagggtgg cataccggac ccatccggat gccggcagtg
     4201 agatagtgat tctttttcga aaaatgtcgg ttgcgaacag caaccctatc acaaccatgg
     4261 gtatccctcc gggaaccgcg gacagcgtca tgtagaaaaa gaagaagacg atgccgttcc
     4321 agaggaagac cagcgccagt gcgcccttga tgagtgcctg cgatcgcatg tccgcgggtc
     4381 tcatgaagac caaagccgtg gtgatcaaga gcaacagcca caatattacg ttgacaggga
     4441 gccagtcggt attgtaggca gcaaactgcg tgaagaaata ggatgcatcc atagtgtaac
     4501 ctcatcccac ccggtcgtgc ggtataacca tatgatggcc gtgattgaga ataaaagtga
     4561 atacctgatg aacctgggtg agccaccaga acgcgagttt tgcagggcgg gggctgccgc
     4621 cccctcgacc ccggggcgac cgtgcggtcg ccgggatgtg aagaggcacc aaaaacccct
     4681 ggagagaagg gaaggaatag aaaagatcag tcgtattcgc gccaggtgtg gccgcattcg
     4741 cagcagcgga agaagcgaac ctcgctctcg tcggcggcac ggagctgccg gagccaccag
     4801 aacgcgagat tatgttcgca cttggggcac ttggtcgcga tggtggggag cgtgcgtacc
     4861 gacccctcct cctcgacgat gacgatctcc ctctcgcgcc gttccttcct gatctgcatc
     4921 tggtcgtcct tgccgatatt gcggatatac ccgcatttcc ggcatttcaa ctggcctgca
     4981 gaggagatca tcaggctcct gcactccgga cagaacatga tggggtatta tgcgttacca
     5041 ttcgagatta aggcttggtt tgtagtaatg tgaattctcc gcatattttg cccgatgcgg
     5101 cgggaagggt gtgcaaacgc actctttttc atccatggac gccaaaataa cagggatgaa
     5161 ggagctcctg gtactcttct cctgcctgtt tttcctggcg ttccttgtgc catggaagct
     5221 ccgcaactgg tgtgcggtgt tcggatggac ctgcatcgtc ctctttctct ttgcagaagt
     5281 cccctattac ttctccatca acaacttcct gtatcccgtt atggcggtgc tctcggtccc
     5341 gttcctcgtg atcaccgcaa ggcacctcat tacacgcgag gtgctggtca tgcagctctc
     5401 caccgcggca gcagtggcct tcctcatcta tgcgccgttt gcctactctc cccttggtga
     5461 ctggctcatc tctgtggtgg tcgggcagac cgcgtggatg ctcgaagcct tcgggtatcc
     5521 tcccctccag cttgcctgga acatgctctc ccggaacaat ttcagggtgg agatcatcct
     5581 tgcctgcacc ggcatccagg ccatcgcaat catgctcggc gtcgtgagcg cggtgaagac
     5641 aaatccccgg cagaaaatgc tctcgttcct cctgatagtt ccgaccatct atatcctgaa
     5701 catcgtccgg aacaccgcgg tcatcatcgc ctacaccgag cagtggttcc ccttcctccc
     5761 ggagatcgcc gggaacggcg agttcgggta cgagagcttc ttctgggccc acaacgtcct
     5821 tgctgaaggc cttgccctcc tgctgcttat cgggatcgcg tggggcctct tccggctcat
     5881 ccccgacctc gcggattttg cgaccggcct cgtgcagctc tactggcgtg acatcaggag
     5941 agcgtttggt aaagatagat gatccgctgg agtgcggaga ggttggtgca gatggcaatc
     6001 accaggaccg agagccagat gaacccggtc agcccgcccg caaggagg
//
LOCUS       DAOQYR010000113         6029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_13415_length_6029_cov_5.472715, whole genome shotgun sequence.
ACCESSION   DAOQYR010000113 DAOQYR010000000
VERSION     DAOQYR010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13415_length_6029_cov_5.472715"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..1888
                     /locus_tag="PKM87_10495"
     CDS             188..1888
                     /locus_tag="PKM87_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNQ30537.1"
                     /translation="MEPQEKTLTGLRDRLAEGRFQLAVLGQFKRGKSTLLNALLGEDI
                     LPSSVIPLTAIPTFIRYGEGRSIHVHFQDQRKEEIYPSDNANWMSKTLLGYVSEDVNP
                     KNAKGVSYVEITHPSPILREVVLIDTPGIGSTHRHNTEMTLNFLPQCDAALFLVSADP
                     PITEVEVDFLRQVREKVARVFFVLNKIDYLSESERQTALAFLKKVLEESIGVTPDGQI
                     FPVSAKQALAAKEKGDAALLDASGLPRITGHLIEFLAREKNRVLQEAVGGKALNVLSD
                     TLLQVNLEMRSLQMPVTDLEQRLALFQKKIDEAKRERQHTQDILNGDHRRVVELLEDG
                     CAALRETSKKHLESIAAAAILSTKELDAQVAEDAIAQEIPVYFEHTLGEVTGKFDREL
                     IAILSGHQQRAAALIESIRVAASTIFEIPHHAPATASPLVLAREPYWVSRKSWSGVLG
                     TISPDLVDKALPRSIREKRIKARIGKEIQTLVVHNVENLRWATLQNIDAAFRVFSREL
                     DEDLAHTIEATYGAIEAAYTRRKEHAEEIADRTQKLQKASGDLQAIIDALMPARSLKA
                     "
     gene            1885..2769
                     /locus_tag="PKM87_10500"
     CDS             1885..2769
                     /locus_tag="PKM87_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30538.1"
                     /translation="MIPDTSCQIPPVATPFLPNIVKYVQERRCPSGGYCFYRLDEPNA
                     GDTFHALQALFLLEEKIHDEETVTFLQGLQQPDGSYSSHAAALCAGRGLQLLGAAPLY
                     DVTPFISRNYPCISPETQVIESFSLFEPLYTWLSLFLLHQVPVPEEWREQITGTIFQF
                     QGETGGFGTPSGTLQDTWQAAEILVVLGYQRENLGISRFIWSCEDPEFGFLGRPGSRP
                     PYLEHLYAGLHVSALLGTTPRYAGACRAFIEQCAHHSGGFVRSIFGGSPTLEFTARAV
                     ESLAILEGRTHRLRTSCG"
     gene            2813..4099
                     /gene="eno"
                     /locus_tag="PKM87_10505"
     CDS             2813..4099
                     /gene="eno"
                     /locus_tag="PKM87_10505"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNQ30539.1"
                     /translation="MSTTTITQIKAREILDSRGNPTVEADVHLACGVMGRAACPSGAS
                     TGTHEAVELRDSVKARYRGKGVLNAVDHVNNEIAKALIGIDAADQEAIDHLMIRLDGT
                     RNKATFGANAILAVSMAVARAAAGAKGMSLWRYLERPGRAIMPVPMMNIMNGGVHANW
                     QGSDFQEYMIVPWGAPDFREALRYGSETYSALKDVLKKKGFSTGVGDEGGFAPAVSSN
                     EEPLALMTEAIEAAGYTPGKEIGFALDPASSGFFHDGKYELRTEGRSLSASEMTGYYR
                     DLVDTYPIVVIEDGLAEDDWIGWKALTQEIGDRVELVGDDLFVTNVDRIRRGIKEGVA
                     NGVLIKLNQIGTVTETIAAIELAYSQGWGAMVSHRSGETVDSFIADFTVAMGTGHLKT
                     GAPARGERVEKYNQLLRIEEEMGSKARYAGRKAFVR"
     gene            4124..4657
                     /locus_tag="PKM87_10510"
     CDS             4124..4657
                     /locus_tag="PKM87_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30540.1"
                     /translation="MTVAGTVEMPHRKAKLTRGTRKSGLDENQERWLCVTFEYIDGLL
                     RDIGEVLDGSPVDSAFPRNVADIPEERRQMIRDTIPPIRQRLVQVLDDLAVPRNQKAI
                     PASRTIRTNLTTIDITLEELKRKDWGIPGSPSGTSEEMRSIIEELREMVSGLERCIDA
                     AMDDDSDVRREGERDGN"
     gene            4647..4787
                     /locus_tag="PKM87_10515"
     CDS             4647..4787
                     /locus_tag="PKM87_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30541.1"
                     /translation="MATDTWAHISRKFEDPDDQIDPWVSALSPWVAEVYQGLLVRRRG
                     RV"
     gene            complement(4792..5535)
                     /locus_tag="PKM87_10520"
     CDS             complement(4792..5535)
                     /locus_tag="PKM87_10520"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HNQ30542.1"
                     /translation="MHIQVEGIEGLPLIHKGDDLPALICELTPFNDGDILSIASSVYS
                     KAKGYTKRLADITPTARAIRIADRTREDPRFIQAVLDASTDILLEYPFVLSEVSTGHI
                     GVRAGVDQSNIEDEMVIFLPPDPMAAADEVREEVYSITEKNIGVIITDTCGRSFRRGQ
                     TGHAVGWSGMTAIRDFRGDTDLFGHVLKITEEAVVDEIAGFANFVMGESNNGVPAVVF
                     RNCGTWQGHDDLYFRREEDILRKLLENAR"
     gene            complement(5849..>6029)
                     /locus_tag="PKM87_10525"
     CDS             complement(5849..>6029)
                     /locus_tag="PKM87_10525"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNQ30543.1"
                     /translation="EKNYLHRERYYSGFKRSIGFPEEVDPDKADGSMNNGVLELTIPK
                     KEPRPEERPRKIEIK"
BASE COUNT         1348 a         1743 c         1737 g         1201 t
ORIGIN      
        1 gaccgttatt ccctgacatg acaggaggag tatcatgccc gacccgatac caaatatcca
       61 caccacttct tcccctgctc caggatcgtt cagccaatca tcccccacat ctccggattc
      121 cacctcccct gatctccctt ccctcatgaa caagggcatc accatccttc gggatttggg
      181 gccggtgatg gaaccgcagg aaaagacact gaccggtctc cgggaccggc ttgccgaggg
      241 caggttccag ctggcggtcc tcgggcagtt caagcggggg aagagcacgc tcctgaatgc
      301 cctcctgggt gaggacatcc tcccgagctc cgtcatcccg ctcaccgcaa tcccgacctt
      361 catccggtat ggcgaggggc gctccatcca cgtccacttc caggaccagc ggaaggaaga
      421 gatctaccct tcggacaacg ccaactggat gagtaagacc cttctcgggt atgtgtccga
      481 agatgtcaac ccgaagaatg cgaagggggt ctcctacgtg gagatcactc atccctcccc
      541 cattctcagg gaggtcgtcc ttatcgatac tcccgggatt ggatcgaccc accggcacaa
      601 taccgagatg accctcaact tcctcccgca gtgtgacgct gccctcttcc tggtttcggc
      661 cgacccacca atcacggaag tcgaggtgga cttcctccgg caggtgcggg agaaggtcgc
      721 ccgggtcttc ttcgtcctga acaagatcga ctacctctcc gagagcgagc gccagactgc
      781 actcgcgttc cttaaaaagg ttctggaaga aagcattggg gtcacgcccg acggccagat
      841 cttcccggtg tcggccaaac aggcactcgc agcaaaggag aagggtgatg ctgccctgct
      901 cgatgcaagc ggcctccccc ggatcaccgg gcacctcatc gaattcctgg cacgggaaaa
      961 aaaccgggtc ctccaggaag cggttggggg aaaggcgctc aatgtgctct ctgacaccct
     1021 gctccaggtc aacctggaga tgcggtccct ccagatgccc gtcaccgatc tcgaacagcg
     1081 cctcgccctc ttccagaaga agattgacga agcgaagcgg gagcgccagc acacccagga
     1141 catcctgaac ggggatcatc ggagagtcgt tgagctcctt gaagacgggt gtgcggcgtt
     1201 gcgggagacc tcaaaaaaac atctggaaag tatcgcagca gcggcgatct tatcgacaaa
     1261 ggagcttgat gcgcaggtcg cggaggacgc aatcgcccag gaaatcccgg tctacttcga
     1321 gcataccctc ggtgaagtca ccggtaagtt cgacagggaa ctgatcgcga tcctgtccgg
     1381 ccaccagcag cgggcggctg ccttgatcga atcgatacgg gtagccgcgt caaccatctt
     1441 cgagatcccg caccacgccc ctgccacagc aagcccgctc gtgcttgccc gggaaccgta
     1501 ctgggtttcg cggaagtcct ggtccggcgt gcttggcacc atctccccgg accttgtcga
     1561 caaggcactc ccccggtcga tccgtgagaa acggatcaaa gcacggatcg ggaaggagat
     1621 ccagaccctc gtcgtccaca atgtcgagaa cctgcggtgg gcaaccctgc agaacatcga
     1681 tgccgcgttc agggtattct cccgcgagct cgatgaggac ctcgcccata ccattgaagc
     1741 cacgtacggg gcaatcgagg cagcctatac gagacggaaa gagcatgcgg aggagatcgc
     1801 agaccggacc cagaaactcc agaaggcatc cggtgatctg caggcaatca tcgatgcact
     1861 catgccggca aggtctttga aggcatgatc ccggatacct cctgccagat ccccccggtt
     1921 gccaccccct tcctcccgaa tatcgtgaag tacgtgcagg aacgccgctg cccgtcaggg
     1981 ggttactgct tctaccggct ggatgaaccg aatgccggtg ataccttcca cgccctgcag
     2041 gcactcttcc tgctcgagga aaagatccac gacgaggaga ccgtgacatt cctgcagggt
     2101 ctccagcaac ctgacggttc gtattcttcc catgcagcgg cactctgtgc aggccgaggg
     2161 ttacagctgc tgggtgcagc acctctctac gatgtaaccc ccttcatctc ccgcaattat
     2221 ccctgcatct ctcctgagac ccaggtgatt gaaagcttct cgctcttcga accactctac
     2281 acgtggttgt ccctgttcct gcttcatcag gtccccgtcc cggaagaatg gagggagcag
     2341 atcaccggga ccattttcca gttccaagga gagaccgggg gttttggaac tccgtccggc
     2401 accttgcagg acacctggca ggcagcagaa atccttgtcg tgctcgggta tcagcgagag
     2461 aacctgggca tcagccgctt catctggtcc tgcgaggacc cggagttcgg gtttctcggg
     2521 aggccgggca gccgaccccc ttacctcgaa cacctgtatg ccgggttgca cgtttcggcg
     2581 ctcctcggga ccactccccg gtatgcagga gcatgcaggg cattcattga gcagtgcgcc
     2641 catcattcgg gagggtttgt acgttccatc ttcggtggtt cccccacctt ggaattcact
     2701 gcccgtgccg tggagtcgct tgcgatcctg gagggaagga ctcatcggct gagaacatca
     2761 tgtggataaa tccaataaag aacgatcaga acgtgtgagg aagaaattgg ccatgtcaac
     2821 aaccacaatc acccagataa aagcccggga gatccttgac tcccggggga acccgactgt
     2881 cgaggcagat gtacaccttg cttgcggtgt gatgggaaga gcagcctgtc cgtccggggc
     2941 atcgacagga acccacgaag cagtcgagct ccgcgatagt gtaaaagccc ggtaccgcgg
     3001 gaagggagta ttgaatgccg tcgaccatgt gaacaacgag atcgcgaagg ccctgatcgg
     3061 gatcgatgct gccgaccagg aagcaatcga tcatttgatg atccggctcg atgggaccag
     3121 gaacaaggcc accttcggtg caaacgcaat cctcgccgtc tcgatggcgg tagcccgtgc
     3181 agccgcaggg gcaaaaggga tgagtctctg gcgttacctg gagcgacctg gcagggcgat
     3241 catgccggtc ccgatgatga acatcatgaa cgggggagtc catgccaact ggcaggggtc
     3301 cgatttccag gagtacatga tcgtcccctg gggagccccg gacttccgcg aggccctccg
     3361 ctacggaagc gagacctaca gcgcactcaa ggatgtcctg aagaagaaag ggttcagtac
     3421 cggtgtcggg gacgaaggtg ggtttgcccc agccgtgtct tcaaacgagg agccccttgc
     3481 cctcatgacc gaggcgatcg aagcagcagg gtacacgcca ggaaaagaga tcgggtttgc
     3541 cctggacccg gcatcgagcg ggttcttcca tgacgggaag tacgaacttc gtaccgaagg
     3601 gaggtcgctt tctgccagtg agatgaccgg gtattaccgg gaccttgtcg acacgtatcc
     3661 catcgtcgtc atcgaggacg ggctcgcgga agatgactgg atcggctgga aagctcttac
     3721 ccaagaaatc ggtgaccggg tggaactggt cggcgacgac ctctttgtta cgaacgtgga
     3781 ccggatccgg agaggtatca aggaaggggt agcgaacggg gtccttatca agctgaacca
     3841 gatcgggacc gtcaccgaga caatcgcggc aatagaactg gcgtactccc aagggtgggg
     3901 agccatggtc tcgcaccgga gcggggagac cgtcgactcg ttcatcgcgg acttcacggt
     3961 agcaatggga actggccacc tcaagaccgg ggccccggcc agaggagagc gggtggagaa
     4021 atacaaccag ctcctccgga tcgaggagga gatggggagc aaggcacggt acgcgggtcg
     4081 gaaggcgttt gtgagatgaa catgggtgct tggtcccgct ctcatgaccg ttgccgggac
     4141 cgttgagatg cctcacagga aagccaaact cacaagggga acaaggaaat ccgggcttga
     4201 tgaaaaccag gagcgatggc tctgcgtcac attcgaatat attgacggct tgctcaggga
     4261 tattggagag gtgctggacg gttcacctgt cgactcggca ttcccccgca atgttgccga
     4321 tatccctgag gaaagacgcc agatgatcag ggacacaatc cctccgatcc ggcaacgcct
     4381 cgtccaggtc ctggatgacc tggcggtccc gagaaaccag aaagcaatcc cggcatcccg
     4441 gactatccgc acgaacctca ccacgattga catcacgctc gaagagctga agcggaagga
     4501 ctggggaatc cctggcagtc catcaggaac ctcagaagag atgaggagta tcatcgaaga
     4561 gttgcgggag atggtttcag gactcgaacg gtgtatcgac gcagcaatgg atgacgattc
     4621 tgatgtcagg agagagggtg agagagatgg caactgatac ctgggcacac atcagccgga
     4681 aattcgagga ccctgacgac cagatagacc catgggtttc agccttgagt ccatgggtcg
     4741 ctgaagtgta tcagggactt cttgtaagac gccgtggccg tgtgtagcat gctacctggc
     4801 gttctccagc agttttctga ggatatcctc ctctctccgg aaatacagat catcatgccc
     4861 ctgccaggtt ccgcagttgc ggaagaccac agctggcaca ccgttgttgc tctctcccat
     4921 cacgaaattg gcaaagccgg cgatctcatc gaccacggcc tcctcggtga tcttgagcac
     4981 atgcccgaag agatcggtat cgccccggaa atcccggatc gcggtcatcc cgctccagcc
     5041 gacggcatgg ccggtctggc ccctccggaa tgagcgcccg caggtgtcgg tgatgatgac
     5101 cccgatattt ttttcggtga ttgagtagac ttcctcgcgg acctcatcgg ctgcagccat
     5161 gggatccggg gggaggaaga tcaccatctc gtcctcgatg ttgctctggt cgactccggc
     5221 acggaccccg atgtgaccgg ttgagacttc agagaggacg aatgggtatt cgaggagaat
     5281 gtcggttgag gcatccagga ccgcctgtat gaaccgtggg tcctcccgtg tccgatctgc
     5341 tatccggatc gcccgagcgg tgggcgtgat atcggcaagc cgttttgtgt atccctttgc
     5401 cttggaatat accgaagaag ctatgctgag gatatcccca tcattgaacg gggtcagttc
     5461 acagatgagg gcagggaggt cgtctccttt gtggatgagg ggaagtccct cgatcccttc
     5521 gacctgaatg tgcatgagaa ggtagttgga tcttttctct gataagctag gtgcaaagtg
     5581 ttgccgcacg gtcatttcgc tataaaaacc accttacact ttttcacccg catttctgcg
     5641 cgagcacaaa aataactgat ttacgggctc tgttgctaac tggctgggtt ttcagaagtt
     5701 catgttcaaa atttcttatg agaagaaata tcttaccata tcagcggccc gagcgagtgc
     5761 cggttacgaa acgcatcagc ggctcatcgt gcggccgcga gtcatcgagc gtctgatata
     5821 gaataccttg ctgaaaaaaa gtgataaatt acttaatctc gatttttcgg ggcctttctt
     5881 caggtcgcgg ttcctttttg ggaatagtca gttcgaggac cccgttgttc atcgatccat
     5941 cggccttatc cggatcgacc tcctcgggaa aaccgatact ccttttgaag ccggaataat
     6001 accgctcccg gtgaagataa ttcttctct
//
LOCUS       DAOQYR010000114         5934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_13701_length_5934_cov_8.704882, whole genome shotgun sequence.
ACCESSION   DAOQYR010000114 DAOQYR010000000
VERSION     DAOQYR010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13701_length_5934_cov_8.704882"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(790..1026)
                     /locus_tag="PKM87_10530"
     CDS             complement(790..1026)
                     /locus_tag="PKM87_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30544.1"
                     /translation="MKFRYGHAVLQGRKARKGIFSETFVILPPRIYPITGIPGLFNFV
                     LQVFQECPLALGRKKRGVFDIQQQLLQPKGQPDK"
     gene            complement(1341..1622)
                     /locus_tag="PKM87_10535"
     CDS             complement(1341..1622)
                     /locus_tag="PKM87_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30545.1"
                     /translation="MRDIPVNGFSIYCLIPNVIRKIPKPMNRMARGRGRISPVPVMKG
                     NIPNKMRRSPRPSTGPPLSTPNTGYDLPTSSEGVFVGPNIGSWYFTDCP"
     gene            1633..2241
                     /locus_tag="PKM87_10540"
     CDS             1633..2241
                     /locus_tag="PKM87_10540"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNQ30546.1"
                     /translation="MKRLTKQGVPVLDRETTSEMLEREARSSPDPHVRGYSLYLLGAE
                     NDVSKKSLFLTSLKDPDKGVRGQAARALAGLGEEAVPDLLHLLGDKEWRTRYRAAEAL
                     GMSGSVNAAAALIRALTDEKDHVRYMAAKSLGLMGVRSAITPLIARLSDENEYVRRMA
                     GHSLEKIGGEEAEIEIRRAVAEAPAGAVRDTLKKMLDKMRQE"
     gene            complement(2245..>2480)
                     /locus_tag="PKM87_10545"
     CDS             complement(2245..>2480)
                     /locus_tag="PKM87_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNQ30547.1"
                     /translation="KLLKLQVDIGDERRQIVAGIAPFYDPADLIGVPVVVVANLAPAK
                     IFGVESRGMLLAAGDRASLLMPRIEVPPGTKIR"
     assembly_gap    2481..2580
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2581..3785)
                     /gene="metG"
                     /locus_tag="PKM87_10550"
     CDS             complement(<2581..3785)
                     /gene="metG"
                     /locus_tag="PKM87_10550"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNQ30548.1"
                     /translation="MSEKPILVTCGLPYTNGPCHIGHLRTYIPADCYVRYLRRRGEEV
                     VFVCGSDNHGTPIVVSAEEAKISPREMSERYHAHFDLTFRRMKVEFDHFGMTDDPTNH
                     SRTKQIVETLAKNGFIYSKVIKQSYCTKCRRFLPDRYVEGTCPHCGVQARGDECDQGC
                     GRHLEPGEIRDPVCRVCKTKAELRDQEHYFFKLSEFKEFLLQYLDGLAGTLNARNYAL
                     GWVKEDLHDWCITRTLDWGVKFPGRDDLVVYVWVDAPIGYISFTEEWAKKTGNDWKTL
                     WCGENRVTHFIGGDIIYHHCIFWPALLKGAGYGTPYSVVASGMVKVDDHKFSKSRGYV
                     VWTNDDYLDRGLPADYLRYYLLSYTNHTKDLNFSWKVFGERVNNELVNTLGNFIYRSL
                     YFAHKEFGG"
     gene            3828..4622
                     /locus_tag="PKM87_10555"
     CDS             3828..4622
                     /locus_tag="PKM87_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30549.1"
                     /translation="MAKKLVFQTVSMGSEPPIPEAGELSEWVRTQRGRQADLTSFLLE
                     EGLLPQKEAEVDFPCSGGTFYHKRMRESISGIKDGYITHELGLMPEFVEDDAARVRSV
                     SGGSRVALPAPHLLDLTDRYYGDVDESSAALCQQYRRLLRAMRDAGVAGHVLHCADAV
                     PEELESLAGKKVFFFLEEPDEESIATLLEYQRVIAIRRDLLPVLLSHREEYEVTEVLI
                     MDPDAMDLKNLNHSFDPDQIRVGGYWNGNNLEYWRDLVDSATLSLQ"
     gene            complement(4611..5585)
                     /locus_tag="PKM87_10560"
     CDS             complement(4611..5585)
                     /locus_tag="PKM87_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HNQ30550.1"
                     /translation="MKFPWTAPPRKGRDEELQDQLQKRPRGVVHITHNDLDAVGCDAI
                     HRRVFDPVTTVFCSVGRFPHVMSEISRMSGEGDTLSISDLGYYQGAESVVDTLREKGW
                     RVEWRDHHRWRDEERARVEKRCNLLCVQVDTCATGICARDLAPGDPVALEVARVVCDY
                     DLWIHADPRSAVLGQVMQRKKNREYVRDCLVQGIFSDARIMAQYREIREEMERLMEKS
                     IRKAHIRGSRYRIAFAPLYGYPSETAAMMRSTLSTDMEVLISPSGRFSLRSVPPVSHI
                     LARNFGGGGHPNASGGNFPFSFWDGVLFRLFGRSPHIDRFVEIAESLE"
     gene            5685..5758
                     /locus_tag="PKM87_10565"
     tRNA            5685..5758
                     /locus_tag="PKM87_10565"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5719..5721,aa:Phe,seq:gaa)
BASE COUNT         1327 a         1582 c         1548 g         1377 t
ORIGIN      
        1 ttttgcctat gactcaattc accaaatgcg atattctttt attgttatgg cgtatctata
       61 gtggtacgga acaccgagaa gagaggctgc aatgcccgac attcactctt ggtgtcacga
      121 aacacgatca gaggagagcg aatatcgcat gaatcataaa tttcttggtg taagtgcaat
      181 catctttgca ctgttcatgg tgttgatgcc ggtatctgcg gcatttgatt ccaggaatct
      241 gaatgcaccc tcatctacat tgggcgtcag cgtgacaaaa caggatgtac tggagaaatt
      301 cagcgccgtg aaagagagcc ccctgttctc gcagtatctg aatgaattgt cccggtatcg
      361 caccacatct gggtcatctc aaacgaacgc agtggttacg gattcagtca ctccgtatgg
      421 atactgcagc agctgcagtg gtgtgaacca gtatacggtc attaaccact actcaaccgg
      481 gccaacctac tatccaagtg caaccgagac tgctgagaga gggtatggct cgatggttgt
      541 cactgggccc tccaccggag gcccttacta cctctgggtt aagcccctgt tcttcgccac
      601 agatgtggaa gactattacg acatgcaatg gcattcttgt ggaattcttc ccgtgaattt
      661 cgataagaat atcctttcag gttcgtattg cctgaaagta acgaaaacca gatcggtaac
      721 gtcagacgca gtgtggtgcg cgagtgccgt tgttgctcca aaccagacca ctgttgtgaa
      781 tttaccctct tacttatcag gctgtccctt tggttgcagt aactgctgct gaatatcaaa
      841 tactcctctc ttttttcttc ccagtgcaag agggcactct tggaatactt gcaggacgaa
      901 attaaacaac ccgggaatcc ctgtgattgg ataaatcctc ggtggcaata tcacaaatgt
      961 ttcactgaaa atcccctttc ttgcttttct cccctggaga actgcgtggc cgtatctgaa
     1021 tttcatactg acttcttaac cgggcatctt tcaacatacg ggtgccttta aaggagaata
     1081 gtcccagatg caaaccgttc cccaatggtg ttcggtgtat cgcctcttgc cctggacatt
     1141 cgcaaatagt aatcacaata agtgccacat tccagggata aaagaaataa gcacgaaggg
     1201 caccgttcct gcacgttgat tcctttccag aaaaagatgc gtaaaaatcc gaaattttca
     1261 aatttttcag atataaatcg tataatgcta tacactgaag cgtgctgtgt atgtcgggga
     1321 ttggtgaaag gaagataatt tcaagggcag tccgtaaaat accaggagcc aatgttgggt
     1381 ccgacaaaaa caccctcaga agaagtggga aggtcgtatc cggtattcgg agtggagagc
     1441 ggcgggccag tgctaggtct tggactcctc ctcattttgt tcgggatatt ccccttcata
     1501 accggaacag gagagatcct tcctcttccc cttgccatcc tgttcattgg ttttgggatc
     1561 tttctgatca cattgggaat caggcagtaa attgaaaaac catttactgg aatatccctc
     1621 attactaaat tcgtgaaacg gttgaccaaa cagggtgtac ctgtgctgga cagggagacg
     1681 acttcggaga tgcttgaaag agaggccagg tcgtcccctg atccgcatgt acgggggtat
     1741 tccctctacc tgctcggggc cgaaaatgac gtctcgaaaa aaagtctctt tctcacttcg
     1801 ttaaaggacc ctgacaaggg ggtgaggggc caggccgccc gcgcccttgc aggccttggt
     1861 gaagaggctg ttccagacct gctccacctc ttgggggata aagaatggag aacccggtac
     1921 agggctgccg aagccctggg gatgagcggt tccgtgaacg cagccgcagc ccttattcgg
     1981 gcactcactg atgaaaaaga ccatgtcagg tacatggcag cgaagtcgct tgggctcatg
     2041 ggagtgcgaa gtgcaatcac tcctctcatt gcccgtctct cggatgagaa cgagtacgta
     2101 cgccgcatgg ctggacactc acttgagaag attggcgggg aagaagccga gattgagatc
     2161 aggcgagcgg tcgcggaagc gccagcagga gctgtgagag ataccctgaa gaaaatgctc
     2221 gataaaatga ggcaggaata gggattacct gatctttgtc cccgggggaa cttctatccg
     2281 tggcattaaa agcgaggccc ggtccccggc ggcaaggagc atcccgcggc tctcgacccc
     2341 gaatatcttt gccggagcaa ggtttgccac cacgactaca ggtaccccaa tgagatctgc
     2401 cgggtcgtaa aatggcgcga tccctgccac aatctgcctt ctctcatcac cgatatccac
     2461 ctggagcttg aggagtttgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 atcccgccga actccttgtg cgcgaaataa aggctccggt agatgaagtt tccgagggtg
     2641 ttcaccagtt cgttgttcac ccgctcgcca aagaccttcc atgagaagtt gaggtctttt
     2701 gtgtggttcg tataggagag gaggtaatac cgcaggtagt cggcgggaag ccccctgtcc
     2761 aggtaatcgt cgtttgtcca cacgacgtac ccgcgggatt tcgagaactt gtggtcatcc
     2821 accttgacca tcccgctggc aacgaccgag tatggcgtcc cataccctgc acccttgaga
     2881 agggccggcc agaagatgca gtggtggtag atgatatccc cgccgataaa atgggtcacg
     2941 cggttctccc cacaccagag ggttttccag tcgtttccgg tctttttcgc ccactcctcg
     3001 gtgaacgaga tgtacccgat gggggcatcc acccacacgt agacgacgag atcgtcccgg
     3061 ccgggaaact tcacccccca gtcgagcgtg cgggtgatgc accagtcgtg caggtcctct
     3121 ttcacccacc cgagtgcata gtttctggca ttcagggttc ccgcaagacc gtcaaggtac
     3181 tggagcagga actccttgaa ctcgctcagt ttgaagaagt agtgttcctg atcgcgaagc
     3241 tcggcctttg tcttgcatac cctgcagacc gggtcccgaa tttctccggg ctcgaggtgg
     3301 cggccgcatc cctggtcgca ttcatcgccc cgggcctgca caccgcaatg tgggcaggtc
     3361 ccctccacgt agcgatctgg aaggaaacgg cgacacttcg tgcaatagct ctgcttgatc
     3421 actttggagt agatgaatcc gttctttgcg agagtttcga cgatctgctt cgtgcgcgag
     3481 tggtttgtgg gatcgtcggt catcccgaaa tggtcgaatt caaccttcat ccggcggaag
     3541 gtcagatcga agtgggcatg gtagcgttca gacatctccc ttggggagat cttcgcctct
     3601 tctgcactga ccacgatggg cgtgccgtgg ttgtccgaac cgcagacgaa taccacctcc
     3661 tcgcctctcc tccgcaggta cctgacgtag cagtcggcgg ggatgtaggt ccgcaggtgc
     3721 ccgatgtgac aggggccatt agtataagga aggccgcaag tcaccagaat gggcttctca
     3781 ctcatcacta tgcttttgat gatgatgcta taaataactg aagagacatg gcgaaaaagc
     3841 tcgtcttcca gacagtttcc atgggttcag agccgcccat cccggaggcc ggcgagcttt
     3901 ccgaatgggt ccgcacacag cggggaaggc aggctgacct cacctcgttt ctcctcgaag
     3961 aagggctcct tccacagaag gaggcggaag tggactttcc ctgttccggc gggactttct
     4021 atcataaaag gatgcgtgag tcgatttccg ggattaaaga tggatatatt acacatgaac
     4081 ttggtttaat gccggaattc gtggaggatg atgccgcgag ggtcaggtct gtttcaggcg
     4141 gctccagggt cgccctccct gcaccccacc tcttagatct gactgaccgt tattatggag
     4201 atgtggacga gtcctctgct gcactctgtc aacagtatcg ccgcctcttg cgggcaatgc
     4261 gggatgcagg ggtagcaggc cacgtgctcc actgcgcaga tgcagtcccc gaagaactgg
     4321 agtccctggc aggaaaaaaa gtcttcttct tcctggagga acctgacgaa gagtctatcg
     4381 ccacgcttct cgagtaccag cgggttatcg ccattaggag ggaccttctc ccagttctcc
     4441 tttcgcacag ggaggaatac gaagttacgg aggttctcat catggacccc gacgccatgg
     4501 atcttaagaa cctgaaccac tcgtttgatc cggatcagat ccgggtgggt gggtattgga
     4561 atggaaataa cctggaatac tggagggacc tcgttgattc cgccacccta tcactccagt
     4621 gattctgcaa tctccacgaa ccggtcgata tggggggatc tcccgaacag ccggaagagg
     4681 acgccatccc agaacgagaa ggggaagttt cctcccgatg cattcgggtg acccccgcct
     4741 ccaaagttcc ttgcaaggat atggcttacg ggggggacag agcggaggga gaacctcccc
     4801 gatggagaga taagcacttc catgtcggtc gaaagggtgc tgcgcatcat ggctgcagtc
     4861 tcgctcgggt acccgtagag tggtgcaaat gcgatgcggt acctgcttcc ccggatatgc
     4921 gcctttcgga tactcttttc catgagcctc tccatttcct cccttatctc ccgatactgt
     4981 gccatgatgc gagcatcgga gaagatcccc tgcaccaggc agtctctcac gtactccctg
     5041 ttcttcttcc gctgcatcac ctgcccgaga actgcagaac gtggatcggc atgtatccac
     5101 aggtcgtagt cacagaccac tcgtgccacc tcgagtgcaa caggatcgcc cggtgcaagg
     5161 tcgcgggcgc agatccccgt cgcgcaggtg tccacctgca cacaaaggag attgcacctc
     5221 ttctctacac gggcccgctc ttcgtcccgc caacggtgat ggtcccgcca ctcgactctc
     5281 cagccctttt cccggagggt atcaaccacg ctttcagccc cctggtagta tccgagatct
     5341 gaaatgctca gagtgtcccc ttccccgctc atcctggaga tttcactcat cacatgcgga
     5401 aaccgcccca ctgagcagaa gaccgtcgta acgggatcaa agactctccg gtggattgca
     5461 tcgcatccga ccgcatcaag gtcgttgtgg gtaatgtgca ctaccccgcg ggggcgcttc
     5521 tgcagctggt cctggagttc ctcgtcgcgg cctttccggg ggggagcagt ccagggaaat
     5581 ttcaccggat ccaccgccac agcgcaggat aaaatgggcc ttgcctcgta ttcatgccag
     5641 aaaacacatc ttttattagt ctgtactgac aatattgttt ccctgcctca gtagctcagt
     5701 tcgggagagc gccagactga agatctggtt gtccccggtt caaatccggg ctgaggcatc
     5761 cggaagcggg gttcaaatcc cggacgcaaa gtttttgtat atcctttctg acccgtttct
     5821 atggtatctt ccacgatagc cactaccgta cgggccgaag ttgcatcttc gcaccgtttc
     5881 tcttgtatca aacccgagta tgggatgcac acgatcgatg ctgcattggc atcg
//
LOCUS       DAOQYR010000115         5882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_13852_length_5882_cov_7.357817, whole genome shotgun sequence.
ACCESSION   DAOQYR010000115 DAOQYR010000000
VERSION     DAOQYR010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13852_length_5882_cov_7.357817"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2699)
                     /locus_tag="PKM87_10570"
     CDS             complement(<1..2699)
                     /locus_tag="PKM87_10570"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNQ30551.1"
                     /translation="MRTERLRWLVILVSLFITVVSGTIGFPLVDLHIGDAGFGNELPG
                     IAPSVFDGGERGLQERVTTMPLLFIENAGQSDDEVEFVVISDGGSIFFTREGVTVKLV
                     TLMEDTPAATLVGYRFVGAEPSPRITGLDPLEGKVNFLVGNEPDGWRTGVPTWRAIRY
                     HDLYRGVDLVYEGTPEGLKSAFTVAPGASPGDITLEYSGILGLVLAEDGSLHIKTSAG
                     ELVESAPVCYQEIDGEVIPVGACYVLKGSSRVGFMIGPYDQTCPLVIDPVMKYGLYLR
                     GIGVAYGRSVALDDTGNAYLTGETFPAPYTMSPGSNDMSGDGTDVVVAKINPDGTVPL
                     YVTYIGGDGDDIGRGIRVDAEGNAYVTGQTASADYPVVNAVKSTLSGPSDAFATRLSQ
                     DGATLVFSTYLGGAQDDAGNAVALDSAGDIYIAGNTMSSDFPGIRVPHNTNYGGNQDA
                     FILKMSGDGSQVAFFEFIGGSKSDSGYGVAVDSEGNSYVTGETFSLNFPVINAMQGSL
                     AGGRLSDAFITKVSPDGSSFVYSTYLGGSQVDSARSIGVDAKGRAHVTGATILGVFPV
                     KDAFQPVAGGCTDAFYSRLSPDGSSLEYSTYIGGVNIDEGKGVAVDECGVAYVTGLTF
                     SPDLPVINPYQRFLGGESPFSVEVSDAYVAKFVPWDTIPEYVTYLGGDGDDVGAAIAI
                     NRQACVDCIAERTCASITGYTDSTNFPVSDPYPISFQPKGEGGFLFTLCDDGESPGMP
                     VADFTAEPTSGCPAPLLVQFTDLSTGIPTSWYWEFGDGATSTLQHPTHTYTTPGTFNV
                     TLTVTNVCGSDSLTKYEYISICPIYVPVANFTAEPTSGCPAPLLVQFTDTSLHDPTSW
                     NWSFGDGSFSTLQHPNHTYTTPGTYNVTLTVTNEAGSDSLTKH"
     gene            2965..3741
                     /locus_tag="PKM87_10575"
     CDS             2965..3741
                     /locus_tag="PKM87_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30552.1"
                     /translation="MNGTYQRVFAREYNGSIATFTESGTHSPVAVLTPGGAICTTLFC
                     AGAVLERQGRKGEFMHARLADSTAAFELHIDRTDQVTADILESMEIPCFATVIGEARI
                     TGKGGAGRCTVRVIEMREVDRYVRDAWVLRTADLTMRRIEDLRTALDEGRRDARLDAL
                     VLHYHLDASRLRDLALMVKEAIKGVNAPHASSPEAGDPVALVLEIIREHGGKTGISLE
                     EVTRKAAKHGLAASEVKQAVESLLLEDECYQPARGVLKVL"
     gene            3738..4505
                     /locus_tag="PKM87_10580"
     CDS             3738..4505
                     /locus_tag="PKM87_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ30553.1"
                     /translation="MRLEFLNAGPALLVEGDVRVLVAGDLHLGIESDLSRHGLHIRSR
                     SRERKERLIACIEAADPELFVLLGDVKHNVPLTSRQEYRELPAILDAFRSKVPLRLLP
                     GNHDPGIEKFFHDDEVLPKTGAVIDGVGYIHGHTCPDPSLQGHLIIAGHHHPMVSIRD
                     EVGCALRAPGYLLAPLDDACLQFARAADTQGEETRVLFIPALNELAGYDIMRTVREPF
                     SPLSRCIKAEQSEVFLADGTFLGPLSSLEDHGDTPDA"
     gene            4483..>5882
                     /locus_tag="PKM87_10585"
     CDS             4483..>5882
                     /locus_tag="PKM87_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNQ30554.1"
                     /translation="MEILRTLDRRVRDCIRARGFSRLSEIQEKAIPLVREGRHLLLIA
                     PTGTGKTESAMIPVFDAMMSHAGDGFRTLYITPLRSLNRDMLQRMEWWCRGLGLSVGV
                     RHGDTPQSERRKQALHPPDLLITTPETLQALFMGKVLRRHLANVRCVVVDEIHELAGS
                     KRGAQLAVALERLREYAGEFQRIGLSATVGNPQDVGRFLCGSRDFSIVSVPVTKHLEI
                     SVDFAGDSFEKQAAWVSRAIGSHESTLVFVNTRVTAEALGHHLYHRGDVEVHHGSLSK
                     DVRIEAEERFRSGSVRALISTSSMELGIDIGRIEHVIQFGSPREVARLVQRVGRAGHQ
                     MDAVSRGTVLATGFDDLLESLVIARRARAGQVEDVSIHLNAADVLANQVAALMVEYRE
                     ISRQRIRDIVERSYPFAMAGNLVDEVCAQMAEHGLIRIEGDMVRSGGRSRRYLSSNLS
                     MIHDERKVVVFDMVAR"
BASE COUNT         1243 a         1647 c         1794 g         1198 t
ORIGIN      
        1 tcgtgcttgg tgagtgagtc tgagccggct tcgtttgtca cggtgagggt gacgttatag
       61 gttcccggtg tggtgtaggt gtggttagga tgctggagag tggagaacga tccgtcaccg
      121 aaggaccagt tccaggatgt gggatcatgg agggaggtat cggtgaactg gacgaggaga
      181 ggcgccgggc agcctgaagt tggctctgca gtgaagtttg ctaccgggac atagatgggg
      241 catatcgaga tgtactcgta ctttgtgaga gagtcagacc cgcagacatt ggtcacggtg
      301 agggtgacat taaaggtgcc gggtgtagtg taggtgtggg tggggtgctg gagggtcgag
      361 gtcgcaccgt caccgaattc ccagtaccag gaggtgggta tccccgtgga aaggtcggtg
      421 aactggacga ggagaggcgc cgggcaacca gaagtaggct ctgcagtgaa atcagcaacc
      481 ggcattccag gcgattcgcc atcgtcgcac agggtaaaga gaaatcctcc ctcccccttt
      541 ggctggaacg agatggggta gggatcgctc acagggaaat tggtagaatc ggtatacccg
      601 gtaatggaag cacacgtgcg ttcggcaatg caatcgacac atgcctggcg gttgattgca
      661 atcgctgctc ccacgtcgtc accgtctccc ccgagatagg tgacatactc gggtatcgtg
      721 tcccatggaa cgaatttcgc gacataggca tcagatacct caacactgaa cggtgattcc
      781 cctccaagga aacgctggta gggattgatg accgggaggt caggagagaa ggtgagcccc
      841 gtcacatatg cgaccccgca ttcgtcgact gcgacacctt ttccttcgtc aatatttact
      901 cccccgatat aggtcgaata ctcaagcgag ctgccgtcag gagagagcct gctgtagaat
      961 gcgtcagtgc agccgcctgc caccggctgg aatgcgtctt tcacagggaa tactccgaga
     1021 atggtcgccc cggtcacgtg tgcacgacct tttgcgtcaa ccccgatgga ccgggccgag
     1081 tccacctggg atccgccgag ataggttgaa tagacgaacg aactcccgtc agggctgacc
     1141 ttggtgatga atgcgtcgct cagcctcccc ccggcaaggg aaccctgcat cgcattgatc
     1201 acggggaagt tgagtgagaa cgtctcgccg gtgacgtagg agtttccctc agaatctacg
     1261 gcaacaccat accctgaatc cgatttactc ccgccgatga actcaaagaa ggccacctgc
     1321 gatccatcac cgctcatctt caggataaac gcgtcctggt ttccgccata gttggtgttg
     1381 tgagggaccc tgattcccgg gaagtccgag gacatcgtat tgcccgcgat atagatatct
     1441 cctgcactat ccagggccac ggcatttccg gcatcatcct gtgccccgcc aaggtatgtc
     1501 gagaagacaa gcgttgcacc atcctgtgaa aggcgggtag cgaacgcgtc actggggccc
     1561 gacagggtgg acttgaccgc attcacgacg ggataatcgg ctgaggcggt ctgcccggtg
     1621 acatatgcat tcccttcggc gtcgacgcgt attccccggc cgatatcatc gccatctccc
     1681 ccgatatagg tgacgtagag aggaacggtt ccgtccgggt taatctttgc gaccaccacg
     1741 tcggttccat caccgctcat gtcattcgag cccggactca tggtgtaggg tgcgggaaag
     1801 gtctcgccgg tcaggtaggc atttcctgta tcatccaggg caacagatct cccgtatgca
     1861 actccaatcc cccgcaggta gagcccatat ttcatcaccg ggtcgatgac aagggggcag
     1921 gtctggtcat acggcccaat cataaagccc accctgctgc ttcctttcag cacgtaacag
     1981 gcaccgacag gtattacctc cccgtcaatc tcctggtaac acacaggagc ggactcaacg
     2041 agttccccgg cggatgtttt tatatgcagt gatccatcct ctgcaaggac caggccgaga
     2101 atgcccgaat attcgagtgt gatatccccc ggggacgcac cgggtgccac ggtgaatgca
     2161 ctcttcaggc cttcaggagt tccctcgtac acgagatcga cacccctgta aaggtcatgg
     2221 taccgtattg cgcgccatgt cgggacgccc gttcgccacc catcaggctc attcccgaca
     2281 aggaaattta cttttccctc aagggggtca agacctgtga tccggggaga tggttccgca
     2341 ccgacgaacc tgtatccaac caatgtcgcg gcaggggtat cctccatcaa tgtcaccagt
     2401 ttgaccgtca cgccttccct ggtgaagaag atggatcccc cgtccgagat gaccacgaac
     2461 tcgacttcat catcgctctg ccctgcattc tcgatgaaga gcaggggcat tgttgttacc
     2521 ctttcctgca gccctctctc tcccccgtca aagacggagg gggcaatacc cggcagttca
     2581 tttccgaaac ctgcatcacc gatgtgcagg tcaaccaggg ggaaaccgat cgtaccggat
     2641 acaactgtaa tgaagagaga gacaagtatg actagccacc ttagcctttc tgtcctcatg
     2701 aaaaaccctg tactgtgatc tggagtaagt tacaatatat aaatgtagtg gtaaaaattt
     2761 ggtgaaaagg acgtattttt tcaatctgcc aataattacc ggcaaggttt gatgcgaaaa
     2821 atatttcagt tggttgctgg cagctgatcg ctgaaaggca ggttgattcc ggtatcagaa
     2881 tggctatctg cattccactc gtacatagtt caggcaggat tggagaggct gagccatgtg
     2941 ccacattcca gagtgtaaaa ttacatgaac ggaacttacc agagggtgtt tgccagggag
     3001 tataatgggt ccatcgcgac atttacggag agtggaaccc actctcccgt tgcagtcctc
     3061 acgccgggag gagcgatctg caccaccctc ttctgtgcgg gagccgtcct ggagcggcag
     3121 ggccggaaag gggagttcat gcatgcacgc ctcgccgatt cgaccgctgc attcgaactc
     3181 cacatcgatc gcaccgacca ggtcacggca gatatccttg agtcgatgga gatcccgtgc
     3241 tttgcaaccg tcattggtga ggcacgtatc acggggaaag ggggggcggg gaggtgcaca
     3301 gtcagggtca tcgagatgcg ggaagttgac cggtatgtaa gggatgcatg ggtattgcgc
     3361 accgcggatc ttaccatgcg gcgcattgag gacctccgca cagcacttga cgaagggagg
     3421 agggatgccc ggcttgatgc ccttgtcttg cactaccatc ttgacgcctc gcggctgaga
     3481 gacctggccc tcatggtgaa agaggcgatt aagggagtga atgccccaca cgcttcctct
     3541 ccggaagcag gggatcccgt ggcactcgtc cttgaaataa tcagggagca tggcggaaag
     3601 actgggattt ccctcgagga ggtaacccgt aaggctgcaa agcacgggct ggccgcgtcc
     3661 gaggtcaaac aggccgtgga atcgctactg ctcgaggacg aatgctacca gcccgcacgg
     3721 ggcgtcctga aagtgttatg aggcttgagt tcctgaacgc aggtcctgca ctccttgtgg
     3781 agggggatgt gcgtgtcctc gtcgcgggag acctccatct tggcatcgag tccgatctct
     3841 cgcggcatgg cctccacatc aggagccgga gcagggagag gaaggagcgg cttatcgcct
     3901 gcattgaagc agccgacccg gaactcttcg tccttcttgg tgacgtgaag cacaatgttc
     3961 ccctcacaag caggcaggag taccgggaac tccccgctat cctggacgca ttccggtcga
     4021 aggttccgct ccgtctgctg ccggggaacc atgatccggg aatcgagaag ttcttccacg
     4081 atgacgaggt gctcccaaaa accggtgcag tgatcgatgg ggtgggatat atccatgggc
     4141 atacctgccc cgatccctcc ttgcagggcc atctcatcat tgccggtcac caccatccta
     4201 tggtgtccat ccgcgacgaa gtggggtgtg cgctcagggc accgggatac ctgctggccc
     4261 cgctggatga tgcatgcctg cagtttgccc gggctgctga tacccagggg gaagagaccc
     4321 gggtgctctt catccccgcg ttgaacgagc ttgcagggta cgacatcatg cgcacggtaa
     4381 gggaaccctt cagccccctc tcccgctgca taaaggccga acagtcggag gtgttcctgg
     4441 cagacgggac gttcctcggg ccgctttcgt ccctggagga ccatggagat actccggacg
     4501 cttgaccgga gggtgcggga ctgcatccgg gctcggggct tctcccggct ctccgagatc
     4561 caggagaagg ccatcccgct ggtcagggag ggccggcacc tccttttgat agcccccacc
     4621 gggaccggaa agacagagag tgccatgatc ccggtctttg acgcgatgat gtcccatgcg
     4681 ggggatgggt tccgcacgct ctacatcacc cccctccggt cgctgaacag ggacatgctt
     4741 cagcgcatgg aatggtggtg caggggactg gggctctcgg ttggggtcag gcacggtgat
     4801 acgccgcagt ctgagcggag gaaacaggcg ctccacccgc cggatctcct gattaccact
     4861 cctgagacgc tccaggccct cttcatgggc aaggtgctgc gaaggcacct cgcgaacgtg
     4921 cggtgcgtgg tggtcgacga gatccacgaa cttgcaggga gcaagcgcgg tgcccagctc
     4981 gcggtcgcgc tcgagcggct gagggagtat gcaggagagt tccagcggat cgggctctcg
     5041 gccacagtgg gaaacccgca ggacgtgggt cgtttcctct gtgggtcacg cgacttctcg
     5101 atcgtgtccg tcccggtcac gaagcacctg gagatatccg tggatttcgc aggggactca
     5161 ttcgagaaac aggccgcatg ggtatcccgg gccatcggca gccacgagtc cacgctcgtc
     5221 ttcgtgaaca ccagggtgac cgccgaggcc ctcggtcacc acctctatca caggggcgac
     5281 gtcgaagtgc accacggatc gctctcgaag gatgtgagga tagaggcaga agagcgcttc
     5341 aggtcgggtt cggtccgtgc cctgatctcc acctcctcga tggaactcgg gatcgatatc
     5401 gggcggatag agcatgtaat ccagtttggg tctccccggg aggtcgcaag gcttgtgcag
     5461 cgggtgggtc gtgccgggca ccagatggac gcagtctcca gggggacggt gcttgccacc
     5521 ggtttcgacg acctgctcga gtccctcgtc attgccaggc gggcgagggc gggccaggtt
     5581 gaggacgtga gtatccacct caatgcagca gatgtcctcg cgaaccaggt ggcggcgctg
     5641 atggtggagt accgcgagat ctcccgccag agaatccggg acatcgtgga gaggagctac
     5701 ccctttgcca tggcaggaaa cctcgttgac gaggtctgtg cccagatggc cgagcacggc
     5761 ctcatccgca tcgagggcga catggtgagg tcggggggga gatcacggcg ctacctctcg
     5821 tcgaacctct ccatgatcca cgacgagcgg aaggtggtcg tctttgacat ggtcgcaagg
     5881 aa
//
LOCUS       DAOQYR010000116         5728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_14332_length_5728_cov_8.814560, whole genome shotgun sequence.
ACCESSION   DAOQYR010000116 DAOQYR010000000
VERSION     DAOQYR010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14332_length_5728_cov_8.814560"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PKM87_10590"
     CDS             <1..360
                     /locus_tag="PKM87_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30555.1"
                     /translation="HRAAACALINVMAGFLCISRVRRACSRECHDACRAALLREIDDR
                     RVGGLEIPSHVMESFPYRATGACDVMLVAGEGLISPEGRSLLEGPDRDRALLVGPSTS
                     GVASLEKLRHWCPYGKG"
     gene            391..1668
                     /locus_tag="PKM87_10595"
     CDS             391..1668
                     /locus_tag="PKM87_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase"
                     /protein_id="HNQ30556.1"
                     /translation="MRFGSSGIRALYGSELVTTALALGRVLGRRHEVVVVGRDTRTTS
                     RIVENAFSAGVLSEGGAVRSAGLVPTPAVAGAVPVSGVGCMVTASHNPEEYNGLKVFN
                     PGGSSLLKSQQEETERLLCEEWKGKGLSLPPPRFGTLTGTGILDSYIGGIIGEVHCAR
                     EAKMVLDCGNGAGGVASPEILKGIGVDRKCINCCPSGHFSRPSEPLEEHVKYLGPLVR
                     RWGADGAVVHDGDADRMMAFDSQGRYISGDQLLVLFTRFLGAKKVVTTTDASMAIEEW
                     AEVKRTPVGDSYVSEELVRGGDLGGEPSGAWIFPRHSLCPDGPYAAAVFCRMVSEWDI
                     AAELDAIPMYPILRASLPCKSATDVMRALGATVPTDGIRVEEEDGWYLVRASGTEPKI
                     RVTAEGRTRAAAEVLLGKGLTAVKQWKKEITHK"
     gene            1681..2880
                     /locus_tag="PKM87_10600"
     CDS             1681..2880
                     /locus_tag="PKM87_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNQ30557.1"
                     /translation="MQCVILAAGEGKRMRPLTATRPKVMLPLANRPMLEHLVLAVCDA
                     GISDLLIVTGYMEAEIRKHFGNGRTLGVGIEYAVQRHQRGTADALLQAREWLEGDFIV
                     MNGDMVVPSADIARVLAADPHCMAVASSDHPEDYGVVQIEGEIITGLQEKAQSPQGNM
                     VNAGLYHFSQDIFPLLDRVGLSPRGERELTDALAMLIRKGELAACRLTSWIDVGYPWD
                     LLSANERMMQEMTASIQGVIEEGVVLKGAVSVGEGSVIRSGTCIEGPCSIGRDCRIGP
                     HAYIRGATTIGDGCHIGHATEVKNSIVLPNTNLPHFNYLGDSVVGSGCNFGAGTKVAN
                     LRHDRAEVKVRGISTKRRKLGAIVGDRVQTGINCSVNVGAVIGCGAQIGPHALVDGYI
                     APGAKIR"
     gene            2891..4048
                     /locus_tag="PKM87_10605"
     CDS             2891..4048
                     /locus_tag="PKM87_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNQ30558.1"
                     /translation="MQAVILAAGEGKRLRPLTRSRPKALIPVGNVPVMDYVIDALLAS
                     GIRDIIVVVGYRKEQVIRHLNSLDASVEVVVQEKQLGTAHALLQAKDLIKGRFMVLPG
                     DNFISSASIARIRDVRNALLVKEHPYPSNFGVVVTRRGYVTSILEKPAHAPSFTISTG
                     IFSLDRDFFSCIQGNDLTDAVGAFIASGKKIRAVPADDWQDAVYPWDLLQMNQKLIHA
                     ASAEKAGEVSGSCVIQGQVRIGKGSRIGPGTCITGPVVIGPGCEIGPHACIMPFSSIG
                     SRVRVEPYTMVENAIVMDDTHIGSHSRIKDTVIGEGTRLEDHVSTVTAAPLMEIQGEQ
                     IRGRFGAILGEKVRAGPFTVFEGAIAGNGVTVQGGSRIQSTMAYEDGGLVI"
     gene            4051..>5728
                     /gene="glmS"
                     /locus_tag="PKM87_10610"
     CDS             4051..>5728
                     /gene="glmS"
                     /locus_tag="PKM87_10610"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNQ30559.1"
                     /translation="MCGIFGYCGKKKAAPILVEGLRRLEYRGYDSFGIAVSNQGITVR
                     KREGKISLGGADLARVKGTMGIGHTRWATHGVPNDQNAHPHLDCQGTIAVVHNGIIEN
                     YAALKRELQDRGHEFRSETDTEVIPHLIEEAFRGDLAAAVGEVIPRLEGSYALLVIAA
                     GCTEMVAVRQKSPLVLGVGDRAFLAASDVTPILEHTERVIFLEDGDIATISTRGIEIQ
                     HGGTGVERPVQRIDWSLAEVKKGGFPHYMQKEIYEQPEVFSLTTRALRGEETPAVLQG
                     IRGVTLVACGTSFNAALLCRYFIEEYCGIPARVELASEFKYYTPPGDYLVVGVSQSGE
                     TADTLAAIEKARMHNHPTLAVTNVLGSTITRLADHTLYMRAGPEISVAATKSFIAELA
                     VFFHLVDRMSEGALESSLSTGHLAIGEALLQDLSPAVELCRGAHSMFFVGRGPFYPLA
                     LEGALKMKEISYIHAEGYAAGEIKHGPFALLSHETPVVALCPPGQAFPVMFSNIREMK
                     ARGTPLIVIGAGGNQDLQEVSDVFIPLPEGGLVSHVLASTALLQLLAYHTA"
BASE COUNT         1132 a         1658 c         1924 g         1014 t
ORIGIN      
        1 caccgggcgg cggcatgcgc cctcatcaac gtgatggccg ggttcctctg catttcgagg
       61 gttcggaggg cgtgttcgcg ggagtgccac gatgcatgca gggcggcgct cctgcgggaa
      121 atcgacgacc gccgcgtggg tggcctggag atcccttccc atgtcatgga atcgttcccc
      181 tatcgcgcca ccggcgcgtg cgacgtgatg ctggtcgccg gcgaggggct gatctccccg
      241 gaaggtcgct cgctcctgga aggtccggac cgcgacaggg cactcctcgt aggcccgtcc
      301 acgtcggggg tcgcaagcct cgagaaactg cgccactggt gcccttacgg aaaaggataa
      361 ctctatttct cccgtttccg attcatatcc atgcggttcg gctcttccgg aatccgggct
      421 ctttacggaa gcgaactcgt caccaccgcg ctcgcgctcg ggagggttct cgggcggcgt
      481 catgaggtgg tagtggtggg gagggatacc cggaccacct cccggatcgt ggagaacgcg
      541 ttttccgcag gcgtgctttc agaaggaggg gcggtgcgct ccgcggggct ggtccccacc
      601 cccgcagtgg cgggggcagt cccggttagc ggtgtgggct gcatggtcac cgcgtcccat
      661 aacccggaag agtacaacgg cctcaaggtg ttcaaccccg gggggtcttc cctcctgaag
      721 tcacagcagg aagagacaga gaggctcctt tgcgaagaat ggaaggggaa agggctttct
      781 ttgcccccgc cgcgtttcgg cacattgacc ggtaccggca tccttgactc ctacattgga
      841 ggcatcatcg gcgaggtgca ctgcgcgcgc gaagcaaaga tggtgctaga ctgcgggaac
      901 ggggccgggg gcgtggccag cccggaaatc ctcaagggga ttggagtcga ccggaaatgc
      961 atcaactgct gcccgtcggg ccatttttca aggccatcgg agccgctcga ggaacacgta
     1021 aaatacctcg gcccactcgt gaggagatgg ggcgcagacg gggcagtggt ccacgacggg
     1081 gacgccgacc gcatgatggc gtttgactcg cagggccgct acatctccgg cgaccaactg
     1141 ctggtcctct tcaccaggtt tctgggtgca aaaaaggtgg tgaccaccac cgatgcgagc
     1201 atggcgatcg aggaatgggc ggaggtgaag cgtaccccgg ttggggacag ttatgtctcg
     1261 gaggaactgg tccggggggg agatctcggg ggcgagccgt cgggtgcatg gatctttcca
     1321 cggcactccc tctgcccgga cgggccgtac gccgcagccg tcttctgccg gatggtctct
     1381 gaatgggaca ttgcggccga gctcgatgcc atccccatgt accccatcct ccgggcctct
     1441 cttccctgta aaagcgcgac cgacgtgatg agggcgcttg gagcaacggt ccccaccgac
     1501 ggcatccggg tcgaggagga ggacggatgg tatctcgtgc gggcttccgg gaccgagccg
     1561 aagatccggg tcacggcgga gggcaggacg cgtgccgcag cggaggtact cctcggaaaa
     1621 ggtctcactg ccgtgaagca gtggaaaaag gagataacgc acaagtgaga ggttatctgt
     1681 atgcaatgcg tcatcctggc tgcaggagag gggaagagga tgcggccgct caccgcgacc
     1741 cggccaaaag tcatgctccc gctcgcaaac cggcccatgc tggaacacct ggtcctggcg
     1801 gtctgcgacg cggggatctc cgatctcctc attgtcacag ggtacatgga ggccgagatc
     1861 cggaaacatt tcgggaatgg ccgcacactc ggagtcggca tcgagtacgc ggtccagcgc
     1921 caccagcgcg gaacggccga tgcgcttctc caggccaggg agtggctcga aggggacttc
     1981 atcgtcatga atggcgatat ggtggtcccc agtgcggata tcgcgagggt tcttgccgct
     2041 gacccgcact gcatggcggt cgcatcttcg gatcacccgg aggattacgg ggtggtccag
     2101 atagagggtg agataatcac cggcctgcag gagaaggccc aatcccccca ggggaacatg
     2161 gtgaacgcgg gattgtatca tttcagccag gatatctttc ccctcctcga cagggtcggg
     2221 ctctccccca ggggcgagcg tgaactcacc gacgcgctgg ccatgctgat ccggaaaggt
     2281 gaacttgccg cgtgcaggtt gacgtcgtgg atagacgtgg gctatccctg ggacctcctc
     2341 tctgcaaacg aacggatgat gcaggagatg accgcctcga tccagggcgt gatcgaagag
     2401 ggcgtggtgc tgaaaggcgc ggtctcggtc ggcgaaggat cggtaatcag gtccggcacc
     2461 tgcatcgagg ggccctgcag catcgggagg gactgccgga tagggcccca tgcctatatc
     2521 cggggggcga ccacgatcgg cgacggctgc cacatcgggc atgcgacaga ggtgaagaac
     2581 tcaattgtgc tcccgaatac gaacctcccg cacttcaact accttggcga ctccgtcgtg
     2641 ggaagcgggt gcaacttcgg tgccgggaca aaggtcgcaa acctccggca cgaccgtgcc
     2701 gaggtgaagg tgcgggggat ctccaccaag cgcaggaaac tcggcgccat cgtgggtgac
     2761 agggtgcaga cggggatcaa ctgttcggtg aacgtgggtg cggtgattgg ctgcggggca
     2821 cagatcgggc cccatgcgct cgtagatgga tatatcgccc cgggggcgaa gatacgatag
     2881 gtgaagcagg atgcaggcag tgattcttgc cgcgggtgaa ggaaagagac tacggccgct
     2941 caccaggagc aggccaaaag ccctgatccc ggtcggcaat gtcccggtga tggactatgt
     3001 catcgacgca ctccttgcct ccggtatcag ggacatcatc gttgtcgtgg gataccggaa
     3061 ggagcaggtg atccgacact taaacagcct cgatgcgagc gtggaggtgg tggtacagga
     3121 gaagcagctc ggcacggccc atgccctgct ccaggcaaaa gacttaataa aagggcgctt
     3181 catggtgctg ccgggagaca acttcatcag cagtgcctct atcgccagga tccgggatgt
     3241 gcgaaacgcc ctgctcgtga aggagcatcc gtacccctcc aacttcgggg tggtggtgac
     3301 ccggagggga tacgtgacca gcatcctcga gaagcctgcc catgcaccga gtttcacgat
     3361 atcaaccggc atattctcgc ttgaccggga cttcttctcg tgcatccagg gaaatgacct
     3421 tactgatgcg gtaggggcgt tcattgcttc cgggaagaag atccgtgcgg ttcctgccga
     3481 tgactggcag gacgcggtct acccgtggga cctcctgcag atgaaccaga agctgatcca
     3541 tgccgcatct gcagagaagg cgggcgaggt gtctggcagt tgcgtgatcc agggacaggt
     3601 ccgcatcggg aaggggagca ggataggacc gggtacctgc atcaccgggc cggtggtgat
     3661 tggacccggg tgcgagatag gcccgcatgc ctgcatcatg ccgttttcaa gcatcggctc
     3721 aagggtgagg gtcgagccgt ataccatggt ggagaatgcc atcgtgatgg acgacaccca
     3781 catcgggtca cactcaagga taaaggacac ggtcattggc gaggggacgc gtcttgaaga
     3841 ccacgtcagc acggtcaccg cggcacccct gatggagata cagggcgagc agatcagggg
     3901 gcggttcggc gcaatactcg gtgagaaagt ccgggccgga ccttttaccg tgttcgaggg
     3961 ggcaattgcg ggcaacggcg tgacggtgca gggcgggtca cgcatccagt cgacgatggc
     4021 ttacgaggac ggcggcctgg tgatataggc gtgtgcggaa tcttcgggta ctgcgggaaa
     4081 aagaaagcgg cgccaatcct ggtggagggg ctgcggcggc tcgagtaccg gggatacgac
     4141 tcgtttggaa ttgcggtgtc aaaccagggc atcacggtaa ggaagaggga agggaagatc
     4201 tcactcggag gtgcggacct tgccagggtg aagggaacga tggggatcgg ccacacccgg
     4261 tgggccacgc acggggtccc gaatgaccag aatgcccacc ctcacctcga ctgccagggt
     4321 accatcgccg tggtccacaa cgggatcatc gagaattacg cagccctgaa gcgggaactc
     4381 caggaccgcg ggcacgagtt ccgctccgag accgacacgg aggtcattcc ccacctcatt
     4441 gaagaggcat tccggggaga cctcgccgcg gcggtgggag aagtcatccc caggctggag
     4501 ggcagctatg cccttctcgt gattgcagcc gggtgcacgg agatggtcgc ggtacgacag
     4561 aagagcccgc tcgtgctggg ggtgggtgac cgggcgttcc tggcggcatc cgacgtcact
     4621 cccattctcg agcataccga gcgggtcatc ttcctcgagg acggggacat cgccactatc
     4681 tcgacaaggg ggatcgagat acagcacggg gggaccgggg tagagaggcc ggtccagagg
     4741 atcgactggt ctcttgccga agtgaagaag ggcgggttcc cccattacat gcagaaggag
     4801 atctacgagc agccggaggt cttctcactc actaccaggg ctctgcgcgg ggaggagacg
     4861 cccgcagtcc tgcagggtat ccggggagtc actctcgtgg cgtgcgggac gtccttcaac
     4921 gccgcgctcc tctgccgtta cttcatcgag gaatactgcg ggatcccggc acgcgttgaa
     4981 cttgcatccg agttcaagta ttatacgcca cccggggatt acctcgtcgt gggggtgagc
     5041 cagtcaggcg agaccgcgga cacccttgcc gcaattgaga aggcccgcat gcacaaccac
     5101 cccactctcg ccgtgacaaa cgtcctcggg agcacgatta ccaggctcgc ggaccacacc
     5161 ctctacatga gggcaggccc cgagatcagc gtcgcggcca caaagtcgtt catcgcggag
     5221 ctcgcggttt ttttccactt ggtcgaccgc atgagcgaag gagcgcttga atcgtctctc
     5281 tcaaccggtc accttgcaat cggggaggca ttgctccagg acctctcgcc cgcggttgaa
     5341 ctatgcaggg gtgcccactc catgttcttc gtgggacgag gcccgttcta tccactggcg
     5401 cttgaaggcg ccctgaagat gaaagagatc agttatatcc acgccgaggg gtacgcggca
     5461 ggggagatca agcacgggcc cttcgccctc ctttcccacg agacgccggt cgtcgcgctc
     5521 tgcccgccgg gccaggcatt tcccgtgatg ttctccaata tcagggagat gaaagccagg
     5581 gggaccccgt tgatcgtgat cggtgcgggg gggaaccagg acctccagga ggtatccgac
     5641 gtgttcatcc cgctcccgga agggggcctg gtctcacacg tgctcgcgtc gaccgcactc
     5701 ctgcagctcc tcgcgtacca caccgcgg
//
LOCUS       DAOQYR010000117         5678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_14500_length_5678_cov_5.918904, whole genome shotgun sequence.
ACCESSION   DAOQYR010000117 DAOQYR010000000
VERSION     DAOQYR010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14500_length_5678_cov_5.918904"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(362..1801)
                     /locus_tag="PKM87_10615"
     CDS             complement(362..1801)
                     /locus_tag="PKM87_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNQ30560.1"
                     /translation="MARGLAEAFEKRGPIRTIGVVGMGYVGIPAAALFADVPQYQKVY
                     GFQRDSATSGYKIAMLNRGESPLGGDEPGLAALVQKVVAAGKFSCTADFSRISSCDAV
                     TIAIQTPFRDPGDLLPDFSALHEGIRLTGRHLSEGTLVVLESTVTPGTTAGTARRILE
                     EESGMVAGEDFGLAHAPERVMVGRLIRNLQEHDRIIGGIDDTSTMRACDLYSPILVQG
                     RIIPMSATAAEVEKTAENTFRDLQIAAANQLALYCEAMGVNFYDVRKGIASLKEEGVT
                     RAILWPGAGVGGHCLTKDTYHLERGAKVLGGNLDWPGGERSLFLTARAINDFMPTHML
                     HLTHDGLARAGKEMKGSKVAILGWAFIADSDDARNTPSEVYRDLVVSEGAEVSVHDPW
                     VEEYPGVAISSDLERVITGADAVAVFTGHREYRALDPGSLKRWAGVEHPVIVDGRNVV
                     DPDSFIRYGWVYKGIGRGDKNGHAVVGCR"
     gene            complement(1802..2716)
                     /locus_tag="PKM87_10620"
     CDS             complement(1802..2716)
                     /locus_tag="PKM87_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNQ30561.1"
                     /translation="MDVGVIGVGLMGRNHVRVYSELKQAGNVYLYDLNREAATGVALA
                     FEATPCDSLDDLLRSVDAVSVCVPTPYHRATAAQVISAGVHMLIEKPICATETEAREL
                     LSLMDHDTIVGVGHIERFNPIVGEIRRIIRKPLFVEMNRHNPASSRPMGSSVIEDLMI
                     HDLDILQHVLFSGGYDLSAAGSLDAASALFRFNSTSAFISASRKSSKKIRRIYIEEED
                     LTLEGDFMNQEVFTYYKPEHYRVENERYVQENIIEKVMVNKVEPLKVELSTFLDCVKR
                     GAPFPVTPEQATRNLALCEEIKSSLQGR"
     gene            complement(2718..3806)
                     /locus_tag="PKM87_10625"
     CDS             complement(2718..3806)
                     /locus_tag="PKM87_10625"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNQ30562.1"
                     /translation="MIPVGKPTLGREEEEAVTRVLRSGMLAQGPEVAGFEDQFARYCG
                     VRHAVGVNSGTAALHAALAALGIGPGHEVIVPAFTFFATGSAVCMCGARPVFADVDPD
                     TFNLSVGSVQELLSQRTHAVIGVHLFGQPCEVRPLRELCDERGIPFIEDAAQAHGAEY
                     HGRRVGGLGTAGCFSFYPTKNMTSGEGGMVTLDDEVLAQRIRMFINHGQEEKYLHTSL
                     GYNFRMSDIAASIGKEQLKKVDGFNLRRREVARYYDSHLRRDGIFTPVVAPGMTHVYH
                     QYVIRVTREFPLSRNELARYLGDKGVGTAVHYPVPLHRQPVFSGFAGSDGCPVADELA
                     GKVLSLPVHPRVSDDERAYICRVIQEVP"
     gene            complement(3803..4405)
                     /locus_tag="PKM87_10630"
     CDS             complement(3803..4405)
                     /locus_tag="PKM87_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNQ30563.1"
                     /translation="MIEYGRNILGKGLQIFEPVIVGFPSRERMHQENYPGVRIGDSAV
                     LRSGTIIYCEVEIGDHFQCGHHVLIRERMRIGRNTSVGSSSVIEGYGSIGDDVRIQSM
                     VFVPTHTEIGDHVFIGPAAVLTNDRYPPTGKPELKGPVIGNFAVIGAHATILPGVRIG
                     EGAAVAAGAVVTRDVPAGKMAVGVPATLRDLPHEMRRDAG"
     gene            4633..5373
                     /locus_tag="PKM87_10635"
     CDS             4633..5373
                     /locus_tag="PKM87_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30564.1"
                     /translation="MPPRGSQGSCLLCRKTITKREAALHVKDCLESSGWPRGQNPSLL
                     IAVQGHRMGSYWLLLLARQDSTLAELDSLIRDVWVECCGHLSGFTIQGQRFTRSAECS
                     EMDMKFPLSRVLSPGVPFLYEYDFGSTTMLDLHVVDSHPVSPPESPLCLLARNTPPKV
                     PCDTCGRPAEFRLRDDDGESCIHLCRRCVAAPDHDPWCIEVISNSPRDSVCGYEEDAG
                     AAVTWYPPGWMHEDLIDPELEALIERIQ"
     gene            complement(5388..>5678)
                     /locus_tag="PKM87_10640"
     CDS             complement(5388..>5678)
                     /locus_tag="PKM87_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30565.1"
                     /translation="SWEFAAIEVWPEEFYRLLQGGAKKRDWGNDPWCRVIWMQAYHSS
                     RERQPGRRIIHSKDAIGLYIEGSGEFVYQVRCSFVKENLSIAPAEYSGKTCE"
BASE COUNT         1163 a         1792 c         1533 g         1190 t
ORIGIN      
        1 ccctcgcggc gacccgtgga ggatctcgag ccatatgaag gaatcgatga ggagagcgct
       61 attcctcatc cttctcctct tcgtgttctt tgtatatgcg ggggagatca agctccggga
      121 tggtcccgaa taaggactcc agggacttga ccgggatgag ctggatcccg tcctcgtcgg
      181 cctccaggag gaatttccgg gtccggtatt gtttacggat tttccccggt attaccaccc
      241 gtccattttt atccataaga ataatttctg ccattaaatg taatattggt taaataaata
      301 tttatgtcat tctcaaaaag ttggtaaaaa tacaatattg gtgagaagat cgaggaactg
      361 gttatcggca tccaacgacc gcatgcccgt ttttgtcccc tctcccgatt cccttgtaca
      421 cccagccgta ccggatgaac gagtcgggat ccacgacatt tctcccgtcc actattaccg
      481 ggtgttcaac tcctgcccat cgcttcagag agccaggatc gagtgcccgg tattcccggt
      541 ggccggtaaa gaccgccaca gcatctgccc cagtaatcac ccgctcaagg tcagaagata
      601 tcgccacgcc ggggtactcc tctacccagg ggtcgtgcac gctcacctct gccccctccg
      661 aaacgacgag atccctgtac acctccgacg gcgtgttcct ggcatcgtcg gagtccgcga
      721 tgaacgccca gccgaggatg gcgaccttcg atcccttcat ctccttcccg gcacgtgcca
      781 gcccgtcgtg ggtcaggtgg agcatgtgcg ttggcatgaa atcgttgatc gcccgggctg
      841 tgagaaacag ggacctctcc ccacccggcc agtcgaggtt cccgccgaga accttggctc
      901 cccgctccag gtggtaggta tccttggtga ggcaatgccc gcccaccccc gcacccggcc
      961 agaggatggc acgggtcacg ccttcctcct tcagggacgc gatccctttc cggacatcgt
     1021 agaagttcac gcccatggcc tcgcagtaga gggcgagctg gttcgcggcc gcgatctgca
     1081 ggtcgcggaa cgtgttttcg gcggtctttt ccacctcggc agcggtggcg gacatcggga
     1141 tgattcgccc ctgcacaagg atgggtgaat agagatcgca tgcccgcatt gtgctcgtgt
     1201 cgtcgatccc tcctatgatc cggtcgtgct cctggaggtt ccgaatcagc ctcccgacca
     1261 tcacccgctc gggtgcatgt gcgaggccga agtcctcccc tgcgaccatc cccgactcct
     1321 cttcgagaat ccggcgggcg gtgccggccg tagtcccggg cgtgaccgtg gattcaagca
     1381 ccacgagcgt gccctcggaa aggtgtctcc ccgtaaggcg gataccctcg tggagggcag
     1441 aaaagtcggg caggagatcc ccagggtccc gaaacggcgt ctggattgcg atggtgaccg
     1501 catcgcacga agagatccgc gaaaaatcgg cggtgcagga aaatttccct gcagcaacga
     1561 ctttttggac cagggcggca agaccgggct cgtcaccgcc gagcgggctc tcgcctcggt
     1621 tcagcatggc gatcttgtag ccggaagtgg cagagtcccg ctggaaccca tacacctttt
     1681 ggtactgcgg gacgtctgca aagagtgcgg cggcaggaat gcccacgtac cccattccca
     1741 ccaccccgat agtccggatt ggccccctct tctcaaaggc ctccgccagt ccccgtgcca
     1801 tctatctccc ctgaaggctc gacttgatct cctcgcagag ggccaggttc ctggtcgcct
     1861 gctcgggcgt gaccgggaac ggcgcccccc tctttacaca gtcgaggaag gtcgaaagct
     1921 ccaccttgag cggctcgacc ttgttcacca tcaccttctc gatgatattc tcctggacgt
     1981 accgctcgtt ctcgacgcgg taatgctcgg gcttgtagta ggtgaagacc tcctggttca
     2041 tgaaatcccc ctcgagcgtg agatcctcct cctcgatgta gatgcgccgg atcttctttg
     2101 aggacttcct gctggcagag atgaaagcag aggtcgagtt gaaacgaaag agcgcggatg
     2161 ccgcatcaag gctcccggcg gcagagagat catacccgcc ggaaaagagc acgtgctgga
     2221 ggatgtcaag gtcatggatc atcaggtcct cgatcactga ggagcccatg ggccgtgacg
     2281 aggcagggtt gtgccggttc atctccacga agaggggctt tcggatgatc cggcggatct
     2341 ctcccacgat gggattgaac cgttcgatgt gcccgacacc cacaatcgtg tcatggtcca
     2401 tgagggagag gagctcccgc gcctctgtct ccgttgcaca gatgggcttc tcgatcagca
     2461 tgtgcacccc ggccgagatc acctgcgctg ccgttgcccg gtgatacggg gtggggacac
     2521 acacgctcac cgcgtcgacc gatctcagta ggtcatcgag cgagtcgcag ggagtcgcct
     2581 cgaaggcaag ggcaaccccc gtggccgcct cgcggttgag gtcgtagaga tacacatttc
     2641 ctgcctgttt caactccgag taaacccgca cgtggtttct tcccatcagc ccgaccccta
     2701 tcaccccaac gtccatttca gggcacctcc tgtatcacgc ggcatatgta cgcacgctcg
     2761 tcatccgaga cccgcgggtg caccggcagg ctgagcacct tccctgcgag ctcgtctgcc
     2821 acggggcacc cgtcactccc ggcaaacccc gagaacacgg gctgccggtg gagggggacg
     2881 gggtaatgca ccgccgtgcc aacccccttg tcccccaggt atctggccag ttcgttgcgg
     2941 gaaaggggga actccctggt caccctgatc acgtactggt ggtacacgtg tgtcatgcca
     3001 ggggcgacca cgggggtgaa aatcccatcc ctgcggaggt gggaatcgta gtaccgtgca
     3061 acctccctcc tccggagatt gaacccgtcg accttcttca actgctcctt tccgatcgaa
     3121 gcggcgatat ctgacatgcg gaagttgtat ccaaggctgg tgtggagata cttctcctcc
     3181 tggccgtggt tgatgaacat gcgtatcctc tgtgccagga cttcgtcgtc aagtgtcacc
     3241 atgccgcctt ccccggaggt catgttcttc gtcgggtaaa aggagaagca ccccgcggtc
     3301 cccagtcccc ccactctcct tccatgatac tcggcaccgt gggcctgtgc ggcgtcctcg
     3361 ataaagggga tccccctctc gtcgcagagt tcacgcagag gcctcacttc gcagggctgg
     3421 ccgaacaggt ggacgccgat caccgcgtgt gtcctctggg agagcagctc ctggacactc
     3481 ccgacagaga ggttgaaggt atccgggtcc acatccgcaa agaccggcct tgccccgcac
     3541 atgcacactg ccgaacccgt ggcaaaaaac gtgaacgcgg ggactatcac ctcgtggccg
     3601 gggccgatcc ccagcgccgc gagggcggca tggagcgctg cggtaccgga attcactcct
     3661 actgcgtgcc gtaccccaca ataccgggca aactggtcct caaacccggc aacttcggga
     3721 ccctgcgcaa gcataccgga ccggagaact cgtgttaccg cctcctcctc ctcccggccg
     3781 agagtcggct tcccgacagg tatcaccctg catccctcct catctcatgg gggaggtccc
     3841 gaagggtggc cggaaccccc accgccatct tcccggcggg gacgtccctc gtgaccaccg
     3901 ctcccgccgc aaccgccgcc ccttctccta tccggacacc gggcagaatc gtcgcatggg
     3961 ctccgatgac cgcgaaattc ccgatcacag ggcctttcag ttcaggtttc ccggtcggag
     4021 ggtagcggtc gtttgtcagg actgccgccg gcccgataaa gacgtggtct ccgatctctg
     4081 tgtgagtggg aacgaacacc atgctctgga tgcggacatc gtccccgata ctcccatatc
     4141 cctcgatcac ggaagaggac ccaacagagg tattccttcc gatgcgcatc ctttcacgaa
     4201 tcagcacgtg gtgtccgcac tggaaatgat caccaatctc aacctcgcaa tagatgatcg
     4261 tgcccgaccg gagaactgcg ctgtccccaa tcctcacgcc gggataattc tcctggtgca
     4321 tcctctccct ggatggaaat cccacaatca ccggttcaaa tatctggaga ccctttccca
     4381 ggatatttct cccgtattca atcatgtgtt cacgatccac cctgatgaga atgggaagga
     4441 gaatatcaac ccatctgaaa tagaaaaacg ccatgatgca caataaatcc atcgtggaaa
     4501 aaagattgtc gcgtgatcga aacaccctta cattcgccag gctcccggaa aaaccctcta
     4561 tacaggagaa cgcgagactg agctgcggaa aaaatccact acattatcgc tctgtacgat
     4621 aacattcaac aaatgccacc aaggggttcc caagggtcct gcctgctgtg caggaagaca
     4681 attacgaaga gagaggccgc attgcacgtc aaggactgcc ttgaatcatc aggctggccc
     4741 aggggccaga acccatcgct tctcatagct gtacagggac accggatggg atcttactgg
     4801 ctcctcctgc ttgcccggca ggatagcacc cttgccgaac tcgactcgct tatccgcgat
     4861 gtctgggtcg aatgttgtgg tcatctcagc ggatttacca tccaggggca aaggttcaca
     4921 cgaagtgcgg agtgtagcga gatggacatg aagtttcctc tctctcgcgt actctccccg
     4981 ggtgttccat tcttatatga atacgatttt ggttcgacga ccatgcttga cctgcacgtc
     5041 gtcgattccc atcccgtctc ccccccggag agccccctct gcctcctcgc gaggaacact
     5101 cctcccaagg ttccctgcga tacatgcgga agaccggcag aattccggct gcgtgatgat
     5161 gatggagaat cctgtattca tctctgccgc aggtgtgttg cagcccctga tcacgatcca
     5221 tggtgcatcg aggtgatcag caactctccg agggatagtg tatgtggata cgaggaagat
     5281 gcgggagctg cggtcacatg gtaccctccc ggatggatgc atgaagacct tattgatccg
     5341 gaactcgaag cactcatcga aagaatccag taggtatgaa cagggcttca ctcacaagtc
     5401 ttcccgctgt attctgcagg tgcaatggac aggttttcct tcacaaaact gcatctgacc
     5461 tggtacacga attccccgct tccctcgata tacagcccga tcgcgtcttt tgaatggata
     5521 atccttcttc ccggttgtct ctccctggaa gagtggtatg cctgcatcca gatcacgcgg
     5581 caccagggat cattgcccca gtcccttttt ttcgcccctc cctgcaacaa cctataaaac
     5641 tcctcaggcc agacctcaat agcagcgaat tcccatga
//
LOCUS       DAOQYR010000118         5662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_14549_length_5662_cov_8.565186, whole genome shotgun sequence.
ACCESSION   DAOQYR010000118 DAOQYR010000000
VERSION     DAOQYR010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14549_length_5662_cov_8.565186"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="PKM87_10645"
     CDS             complement(<1..934)
                     /locus_tag="PKM87_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ30566.1"
                     /translation="MVPNRPYSVMYVDDDQDLLDLGKIFLERNGDFSVDCIDSAPAAL
                     EVLGNAAFDAIISDYQMPGMDGIEFLKSLRARGSGIPFVIFTGKGREEVVIDALNAGA
                     DFYLQKGGDPKVQFAELTHKVRQAIEKRRAEQSLIESKKRLADIIDFLPDATFAIDRE
                     GSVIAWNRAMEEMTGTPAIAVLGRGNHVYSRAFYGTLRPMLIDLVLSPDADFERQHYH
                     FTQRNGTFLTAETTVGRQDGRTIHYWGKACRLYDEDGNLAGAIEAIRDITERVVAETE
                     IREKTRELDLFFQNAIDLLCIADTDGYFRKLNPEW"
     gene            1078..1971
                     /locus_tag="PKM87_10650"
     CDS             1078..1971
                     /locus_tag="PKM87_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNQ30567.1"
                     /translation="MATLRGYADLLRLHFFFAWPLLFCSGYLLAAATYGTFSLADLIR
                     AAIIGFLGFEGGLVLNDYIDREYDKKDTEFGRLTRYWRIFGTRPIPAGLVSPRSALLL
                     FLILAVSTLIVIATLPFPHSLYVALIMLCGYGTEAFYQVRKRNQSFPIAQLVGRTDFA
                     LFPVAGYLCTGFPDTTALCYFLFFYPFAMAHLGVNDLADVENDRARGMQTIPILYGMS
                     GTVWWIVVFLLIHMAAAFLFITRLDPIATTGVALGFALLVFAAVHLARQPTPEGGMKV
                     LPLFHLAMLIYAGSIVIGSLL"
     gene            2253..2573
                     /locus_tag="PKM87_10655"
     CDS             2253..2573
                     /locus_tag="PKM87_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30568.1"
                     /translation="MKMPDNAQLIRAAGLDGWVLPGRTYPHPLPEGLRDFYCYTRDGG
                     HSLLVVLGNEYRHGEPPERFVVPAPVKMVLRHGFQQRDGYLWSDLPYAKDIGLQVRDE
                     DIEF"
     gene            2745..3461
                     /locus_tag="PKM87_10660"
     CDS             2745..3461
                     /locus_tag="PKM87_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ30569.1"
                     /translation="MYAGEQPIEVITIRLGLTSRAYLVRQHGAVLVDTGVPGREGAIV
                     ARMKEAGIHPQDLRLIILTHGHADHAGSARALRELSGAPVAVHRDDASMVRSGRQGAL
                     VPTGTAGLVLGVLYGRKGSAHFHPFEPDILLGDSFALNEYGIRGEVIHTPGHTDGSVS
                     LLLGNGDAIVGDLIFPSIPTGRPGLPFFAGDPEEVRASIGKVLAFQGGTIYPGHGGPF
                     SSARVRHCFPGNPPKSPGQA"
     gene            3582..5069
                     /locus_tag="PKM87_10665"
     CDS             3582..5069
                     /locus_tag="PKM87_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNQ30570.1"
                     /translation="MTHLRPFHYLALTGALLCISCMIAGCTSTDHGSADQHTAPACAV
                     GEIVFLTEELYPFSYAGLDGVARGQSVEVVRDLVRRLNCTAEIRVLPWSVAHDTAVST
                     PGYAVFSTVKTPEREAKFSWVGPIALLEYSLYARNGSNDMLPSLEAARNAGAIAVVDG
                     DARHDFLVKNNFSNIRTYPSDEECLEALLDGSVSLWMGTSATTPGTLARHAVPGGTVI
                     PVYTLLRTELFIAFNRETSAATIDAYQDALDASKADGTFSRITGAGLPASLSPSVDGQ
                     RDTFASETLLPAFSALISARVHGIAAAMETLALTGELKEGDWEKIRPLLVRLEQEYPE
                     ARFWYARPDGSYYTTVDNLTSANLRDRPYFPGVLAGKTSIGTIVVSKSTGRYTAIIAV
                     PVRDKGEVNGILGTSVYCDTLEKALFEEFALPAGYYAFAVDALGMPVIDSLPDRIFSL
                     DESTRSAVREMREGQVDYSFEGAGHQAVFRTEEMTGWKVGIGWGA"
     gene            complement(5097..5621)
                     /locus_tag="PKM87_10670"
     CDS             complement(5097..5621)
                     /locus_tag="PKM87_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNQ30571.1"
                     /translation="MRTNRHQYFLDLALRCAHQGTCLRRNFGAIIVDEFQTIVSTGYT
                     GAPRKQMDCTEIGRCWRKDHHIPSGSNYERCRSVHAEMNALLQAGKLARGCTLYLAGF
                     DVETGDLTQIWPCFLCSKMIVNSGVRSVIMRTGDETYKEMDPIALYQMRSIEALGEEG
                     LKVGRSFLEDAGRN"
BASE COUNT         1079 a         1738 c         1631 g         1214 t
ORIGIN      
        1 cccactcggg gttcagcttc cggaaatatc cgtccgtgtc agcgatgcag agcaggtcga
       61 ttgcattctg gaaaaagagg tcgagttcgc gggtcttctc cctgatctcc gtctctgcaa
      121 ccacccgttc cgtgatgtcc cggattgctt ctatcgcacc tgcaaggttc ccgtcttcgt
      181 cgtagaggcg gcacgctttc ccccagtagt gaatcgtcct tccgtcttgc ctcccgactg
      241 tcgtctctgc agtcaggaat gtaccgttcc tctgcgtgaa atggtaatgc tgtctttcaa
      301 aatctgcgtc aggggagagg acaagatcga taagcatggg gcgcagtgtt ccgtagaacg
      361 ctcttgaata gacatgattg cccctgccca ggaccgcaat ggcaggggtg ccggtcatct
      421 cctccattgc ccggttccac gcgatcacgg atccttcccg gtcaatcgca aaagtggcgt
      481 ccggaaggaa gtcgatgatg tcggcgagtc gcttctttga ttcgatgagc gactgctcgg
      541 cgcgcctttt ctcaattgcc tggcggacct tgtgggtgag ttccgcgaac tggacctttg
      601 ggtctccccc cttctggagg tagaaatccg cccccgcgtt gagggcatcg atcaccacct
      661 cctctctccc tttgccggtg aagatgacaa aaggtatccc ggaaccccgg gcccggaggg
      721 atttgaggaa ctcgattccg tccatcccag gcatctggta atctgatatg atggcatcaa
      781 atgcggcatt ccccaggact tcaagtgctg ccggggcaga atcgatgcag tcaacggaaa
      841 aatccccgtt ccgctccagg aagatctttc ccaggtccag gagatcctgg tcatcatcca
      901 catacatgac agagtaggga cgatttggga ccatacctag aactctcttt ttccagtaat
      961 gaacactgtg gaatatgcgc cagaggagaa ggtcactcct aggctcccat tcgcgacctc
     1021 ctcagtttct cccaatcaga gggtgtatgt accaggacta tactacacta ctacagcatg
     1081 gcgacgctgc ggggatatgc cgacctgctc aggctccatt tcttctttgc ctggccgctc
     1141 ctcttctgct cgggctacct tcttgcggcc gccacgtatg gcaccttctc tcttgccgac
     1201 cttatcaggg ccgcaatcat cgggttcctc gggttcgagg gagggctcgt cctgaacgac
     1261 tacatcgatc gcgaatatga caagaaagac accgagttcg ggaggctcac caggtactgg
     1321 aggatcttcg ggacacgccc tattccggcg gggctcgtct ctccacggag tgccctcctc
     1381 ctcttcctga tcctggccgt cagcaccctt attgtcatcg ccaccctccc gttcccgcac
     1441 tctctttacg tcgcgctcat catgctctgc ggctatggta cggaggcgtt ctaccaggtc
     1501 aggaagagga accagtcgtt tcccatcgcg caactggtgg ggaggaccga ttttgccctc
     1561 tttcccgtcg cggggtacct ctgcacgggg ttcccggata cgaccgcatt gtgctacttc
     1621 ctctttttct acccgttcgc catggcacac cttggggtaa atgaccttgc cgatgtcgag
     1681 aacgaccgtg cacggggcat gcagaccatc ccgatcctgt atggcatgag cgggaccgtg
     1741 tggtggattg tcgtgtttct cctaatccac atggccgcgg cattcctgtt catcacgagg
     1801 ctcgatccca tcgcaaccac cggcgtggca ttagggtttg cgctcctcgt gtttgcggca
     1861 gttcacctgg ccaggcagcc aacccccgaa gggggaatga aggtcctgcc gctctttcac
     1921 ctcgcaatgc tcatctatgc aggctccatc gtaattggat cactcctgta agcaggtgtt
     1981 atgctcctgc aggtggctgc ccctggcact cccctcccca cttcacctca cgggcgccgc
     2041 ctcgcaggag aattcctatg tgttctcggg tggaaagagc gatacctctt gcctggaagc
     2101 ctgcgttctc ccctgcaaag acccagggtg gaggaaaaag gcctcggatg gaccaccacc
     2161 tccattttgc tcccttgata tagtttccct ggcaacatca gggagggttt ggcaggaata
     2221 tcagttttat cactgccgga gatccacgca tcatgaaaat gccggacaat gcgcagttga
     2281 tcagggcggc aggactcgac ggctgggtcc tgccggggag gacgtatccc caccccctcc
     2341 ccgagggatt gcgggacttc tactgctaca cgcgggacgg cgggcattcc ctgcttgtcg
     2401 tgcttggaaa cgagtatcgc cacggtgagc ctcccgaacg gttcgtcgtc ccggcaccag
     2461 tgaagatggt gctcaggcac ggctttcaac agagggacgg ctacctttgg tcggacctcc
     2521 cctatgcaaa ggacatcggc ctgcaggtga gggacgagga tatcgagttt taacgaaaca
     2581 ccatcctttt ttcggggtcc cggaatgtct cccggaggca ggaccagggg aatcagggcc
     2641 tgttttttcc ctcctttttc caaccatggg tgaaagatgt cccccgcgat tcgcagcaat
     2701 cttcttatat ttcccgggcc atcatcagca cagggggtgc gaggatgtat gcaggggagc
     2761 agccaatcga ggtcatcacc atccggctcg gcctgacctc aagggcatac ctggtcagac
     2821 agcatggcgc agtcctggtc gatacgggtg ttccggggag agagggtgcg atcgtagcga
     2881 ggatgaagga agcgggtata caccctcagg acctcaggct cataatcctc acgcacggcc
     2941 acgcagacca cgcgggatcg gcccgggcac tccgggaact gtcaggggca cccgtggccg
     3001 tgcacaggga cgatgcatcc atggtgcggt ccgggcggca gggcgccctc gtccccacgg
     3061 ggaccgcggg acttgtcctc ggggtactgt atggcagaaa gggcagcgct cactttcatc
     3121 ccttcgagcc tgatattctt ctcggcgatt cgttcgctct caatgaatac gggataaggg
     3181 gcgaggtcat ccacacgccc gggcataccg acggctccgt ctcccttttg ctcggaaacg
     3241 gggacgcgat cgtgggagac ctcatcttcc cgagcattcc gaccggaagg ccggggttgc
     3301 cgttctttgc cggcgacccg gaggaggtgc gggccagcat agggaaagtg ctggcgtttc
     3361 agggcgggac gatctatccc ggccacggag gaccgttctc ctcagcccgc gttcggcatt
     3421 gttttccggg gaatccccca aagtcccccg gacaggcttg acgcgcgggt ttttccttct
     3481 gccggacggt tcacactgga acaggaccgc agcatggagc ggcagaatcg aacatgtttt
     3541 ggcggttcag tatagacttc ttctgcaagg agagacttct catgacccat ttgagaccgt
     3601 ttcattacct ggcactcaca ggtgcgctcc tctgcatctc ctgcatgatt gcggggtgca
     3661 cttcaacgga ccacgggtca gctgaccagc acaccgcacc cgcctgtgcc gttggggaga
     3721 tcgtcttcct gacagaggaa ctctaccctt tcagttacgc agggcttgac ggcgttgccc
     3781 gcggacagtc cgttgaagtg gtaagagacc tggtccggcg cctcaactgc acggccgaga
     3841 tcagagtgct cccgtggagt gttgcgcacg ataccgcggt ttccacaccc ggatatgcgg
     3901 tcttttcaac cgtgaagact cccgagcgcg aggccaagtt ctcctgggtc ggcccgattg
     3961 ccctgctgga gtactccctc tatgcaagga acggatccaa tgacatgctc ccgagcctcg
     4021 aggccgcacg gaatgcgggc gcgatcgcgg tggtcgacgg cgacgcacgg cacgatttcc
     4081 tggtcaagaa caatttctcc aacatccgga cgtacccttc agacgaggaa tgcctggagg
     4141 ctctcctgga cggatcggtc tccctctgga tgggcacatc cgcgaccacg cccgggacac
     4201 ttgcaagaca cgcggttccc gggggaaccg tgatcccggt ctacaccctt ctcaggaccg
     4261 agctcttcat cgcgttcaac agggaaacct ccgctgcaac gatcgatgca taccaggatg
     4321 ccctcgatgc ctcgaaggca gacgggacgt tttcccgcat cacaggtgcc ggactccccg
     4381 cctccctttc gccctctgtt gatggtcagc gggacacgtt cgcgtccgaa acgctgctcc
     4441 ctgcgttctc cgcgctcatc tcggcacggg tccacgggat cgccgccgcg atggagacac
     4501 tcgccctcac aggggaactc aaagaaggcg actgggagaa gatccgcccg ctcctcgtca
     4561 ggctcgaaca ggagtatccc gaggcccggt tctggtacgc gcgacccgac ggctcgtact
     4621 atacgacggt cgacaacctg acgagcgcaa acctccggga ccgcccctat ttcccgggtg
     4681 tgcttgccgg gaagacctcg attgggacca tcgtcgtcag caaatcgacc gggaggtata
     4741 ccgcaatcat cgcggtcccg gtccgggaca agggagaggt gaatgggatc ctcgggacat
     4801 cggtgtactg cgacaccctt gagaaggcac tcttcgaaga gttcgcgctc cccgcagggt
     4861 attatgcatt cgcggtggat gccctgggga tgccggtcat agactccctc ccggacagga
     4921 tcttctcact cgatgagagc accagatcag cggtgcggga gatgcgcgaa gggcaggtgg
     4981 actattcttt cgaaggagcc ggacaccagg cagtctttcg gaccgaggag atgaccgggt
     5041 ggaaggtcgg catcgggtgg ggagcctgac ggatcctgcc tgaccaccag gtatcatcaa
     5101 tttcttcccg catcttccag aaaactccgg cccactttaa gcccctcctc tcccagcgcc
     5161 tcgatactcc gcatctggta gagggcgatg gggtccattt ccttgtacgt ctcgtcgccg
     5221 gtgcgcatga tcaccgatct cacccccgag ttcacgatca tcttggaaca gaggaagcag
     5281 ggccagatct gcgtcaggtc tcctgtctcg acatcgaacc cggcaagata aagcgtgcag
     5341 ccccgtgcga gttttcctgc ctggagaagc gcgttcatct cggcatggac gctccggcac
     5401 cgctcgtagt tcgagcccga gggaatgtgg tggtccttcc tccagcatcg gccaatctcg
     5461 gtgcagtcca tctgcttccg gggcgccccc gtgtacccgg tggagacgat ggtctggaac
     5521 tcgtcgacga tgatggctcc gaagtttcgc cggaggcacg tcccctggtg tgcgcaccgg
     5581 agggcgaggt caaggaagta ctggtgccgg ttggtccgca tatacgaata gattgccctg
     5641 aacagggtat caacatgcct gt
//
LOCUS       DAOQYR010000119         5652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_14582_length_5652_cov_9.420761, whole genome shotgun sequence.
ACCESSION   DAOQYR010000119 DAOQYR010000000
VERSION     DAOQYR010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14582_length_5652_cov_9.420761"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PKM87_10675"
     CDS             complement(<1..888)
                     /locus_tag="PKM87_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonyl-tRNA synthetase editing
                     domain-containing protein"
                     /protein_id="HNQ30572.1"
                     /translation="MRLLLIHSDFIEFEARKKTGMAEEGQPLKGFKEEALTVFCAVEA
                     GDEDDIPGVVELAGEEIGKTADQLSVENIVLYPYAHLSSDLARPDAAIEALKMLEHLL
                     VDIGFEVTRAPFGWYKSFRISCKGHPLSELSKTILPPEEGKKANKKEITHEFFVLTPD
                     GTRHEPKQFMDGSSFGCLLKKELGEPSEGGGEPIHVELMRGKELVDYEPVSDVGHLRW
                     MPRGKLVRDLLADYVLQKVLPYGATPVETPVMYDLGDRAIYEHAEKFGERQYRFKSGT
                     RSMMLRFAACFGMFSIMRDM"
     gene            complement(954..1511)
                     /gene="dcd"
                     /locus_tag="PKM87_10680"
     CDS             complement(954..1511)
                     /gene="dcd"
                     /locus_tag="PKM87_10680"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNQ30573.1"
                     /translation="MILVDWQILDRIRRGFISVEPFDPRLVQPNSLDIRLGNHFVWYE
                     KGDGVIDPYQEETIATGTLETTADSIVLAPGQFMLAETHEVIGLPDNIVASIEGKSSI
                     ARLGIELHQTGGWIDAGFRGSITLEMCNVNQRPVRIYAGMPIGQLVFYTTEKALLPYD
                     KKEDAKYMDQRQATLSRYHQNLQEL"
     gene            complement(1589..2563)
                     /locus_tag="PKM87_10685"
     CDS             complement(1589..2563)
                     /locus_tag="PKM87_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNQ30574.1"
                     /translation="MQPRLLCGLACLSLAAALLLCGCTTPESPPDIPAAMTPEDLAGF
                     VHEAAAYAGHAGKEPALSEFNRNPGAFTRGDLYVYAYDYHGTLLAHPYQRESVGTNRV
                     DWTDARGLPAIRIGAHVAAHGGGFIAYLYPAPSASGINESSAESYVPKIGYAYPAGGD
                     WWIASGIYFSDLEDARPGKYPDAVAGMVDLVERGAAYGRGHGDEAAFAEISNASGMFV
                     DADAHYLYAYDYNGTLLAHPHLKDAIGTSLIERTDPFGMKNIRALCDTARSGGGFIVF
                     VWPNPVRENRQELKIGYVLPVDDRWWVGSGVYLSEVTGVDSSFPLPSP"
     gene            complement(2771..3520)
                     /locus_tag="PKM87_10690"
     CDS             complement(2771..3520)
                     /locus_tag="PKM87_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNQ30575.1"
                     /translation="MPEVDFVLVQPLYEGNVGFAARAIKNFGFSRLVLVDPCPLGDDA
                     IARAGHAKDVLESASRLTLDEVFSGSDLVIATTGEVSKSICNPMRMPYYSPREIRSLV
                     EPHRGKVSLLFGRENWGLNNREIRRSDVICTIPTSPDYPILNLSHAVAILAYELADLP
                     RGEYLLAGRTQMEHLYQHIDQYLTLIDHPDFKRDGTMLMMRRILGRANLTAREASTLH
                     GLMRRSEWYIRPDTRAPDDDIEDGTQDIRED"
     gene            complement(3528..4214)
                     /locus_tag="PKM87_10695"
     CDS             complement(3528..4214)
                     /locus_tag="PKM87_10695"
                     /EC_number="2.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerol geranylgeranyltransferase"
                     /protein_id="HNQ30576.1"
                     /translation="MHINWKSWNHVTKLDPDKHLSPEQITGVVTSGTDALMLSGTLNV
                     TRENMQELLRQVARYGLPLVVEPAGPEAVIAEGIDFLFIPSVLNTQDVRWIVGKHHQW
                     VQQHEIDWERVVPEAYIVLNPDSSVGKVTRSNCSLSPAEVAAFATVADRYFRFPIVYI
                     EYSGMYGDPEVVRAVSEALDSSVLYYGGGINSTERASEMAKYADTIVVGNAIYDQGID
                     VLKATVKAVQ"
     gene            complement(4229..>5652)
                     /locus_tag="PKM87_10700"
     CDS             complement(4229..>5652)
                     /locus_tag="PKM87_10700"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNQ30577.1"
                     /translation="IQRMEELGLGTKSTRHEVIQKLVSRKYVEGNPLRPTLVGRAVIE
                     SLEHHADTITRPDMTQTLDAHMQQIKQKARSRDDVVAESRGMLHQVFDQLEAHSQEIG
                     DEIMDQTAEELTLGPCPVCSHDLRIRHLRNQTQFIGCTHYPACSFNIGLPMTMWGFAV
                     RTDEVCPAHRLHFVRLVRKGARPWDIGCPLCHHISSHKETLALLPSFSDELHQKMVAA
                     HIYTVYDLANTPAEVLAHALGIARDEVVRLQAEAADALELLRRRSDCRKFVRSILPPR
                     KGRSPASVIRKLHESGINDIEGLGLVDRSVLRQAGVGEKEAESLQSEAKKVAGERRLK
                     EIGLPAVSLKKYREAGFARPEDMLAAHPLYISFRTGISPETVFRHVEAVASVLGRPVP
                     DKVSRAQYEKGKSELLGIKGVTESTLEALLRAGVYNIESLHETGESALSARAGIPRER
                     VRELRAAAPFKKKKKQSDDIIVI"
BASE COUNT         1001 a         1703 c         1763 g         1185 t
ORIGIN      
        1 catgtcccgc atgatagaaa acatcccaaa gcacgcggcg aaccggagca tcatgctcct
       61 cgtcccgctc ttgaaacggt actgccgctc cccgaacttt tcggcgtgct cgtagatagc
      121 ccggtcgccg aggtcgtaca tcaccggcgt ctcgaccggc gtcgccccgt agggcagcac
      181 cttctggagg acatagtccg cgaggaggtc gcggaccaat ttcccccttg gcatccagcg
      241 gaggtggccg acatccgaga cgggttcgta gtccacaagc tccttgcccc tcatcagctc
      301 gacgtggatg ggctcgccgc cgccttcgct gggctccccg agctcctttt tgaggaggca
      361 tccaaaggag gagccgtcca tgaactgctt cggctcgtgc cgcgttccgt ccggggtgag
      421 gacaaaaaac tcgtgggtga tctccttttt gttggccttt ttgccctcct cgggcgggag
      481 gattgtcttc gagagctccg agagggggtg gcccttgcac gagatgcgga aggacttgta
      541 ccacccgaag ggcgcccggg tcacctcgaa accgatgtcc accaggaggt gttcgagcat
      601 cttcaatgcc tcgatggcgg catccggcct cgccaggtcg ctcgagaggt gggcataggg
      661 gtagaggacg atgttctcca ccgagagctg gtcagcggtc tttccgatct cttcgccagc
      721 gagctcgacg acaccgggga tgtcgtcctc gtcaccggct tcgacggcgc agaagactgt
      781 cagggcctcc tccttgaatc ccttcagtgg ctgcccttct tctgccatcc cggtcttttt
      841 cctggcctcg aattcgataa aatctgagtg tatcaagaga agtcgcattc attcagctcc
      901 atttgcggtc agatcatccc agataattgt ggtttcatgg tgtatatact cattcagagt
      961 tcctgcaggt tctggtggta ccgagagagc gtggcctgcc gctggtccat gtacttggcg
     1021 tcctcctttt tatcgtaggg gaggagtgct ttttcagtgg tgtaaaagac cagctgcccg
     1081 atgggcatcc ccgcgtatat cctcaccggc cgctggttca cgttgcacat ctcgagcgta
     1141 atggagccgc ggaacccggc atcgatccag ccccctgtct ggtgcagctc tattcccagg
     1201 cgtgcgatgc tgctcttccc ctctatgctc gccacgatat tatcaggaag gccgatcacc
     1261 tcgtgggttt cggcgagcat gaactggccc ggcgcgagga cgatagagtc agcggtggtc
     1321 tccagtgtcc cggtagcaat cgtctcctcc tggtagggat cgatcacccc gtcacccttc
     1381 tcgtaccaga caaagtggtt cccgaggcgg atgtcaaggg aattgggctg gaccaggcgg
     1441 ggatcgaagg gctccaccga gatgaacccg cgccggatgc ggtccagaat ctgccagtcc
     1501 acgagaatca tgagggaagg tttagaccgc gatgagcatt agggttcccc tggccctcca
     1561 tcggggcagt cttcatttcc cggaaaaatc agggcgaggg gaggggaaac gagctgtcca
     1621 ctcccgtgac ctcgcttaaa tagactcctg agccaaccca ccacctgtcg tcgacaggga
     1681 ggacataccc gatcttcagt tcctgccggt tctctctcac ggggttgggc cagacgaaga
     1741 cgatgaaccc gccgccggag cgggcagtgt cacagagcgc ccggatattc ttcataccga
     1801 atggatcggt cctctcaatg aggctggtcc cgattgcatc cttcaggtgg ggatgggcaa
     1861 ggagcgtccc gttgtagtcg tatgcataca ggtagtgggc gtcggcatcc acgaacattc
     1921 ccgacgcgtt cgagatctct gcaaacgccg cctcatcgcc atgcccgcgc ccataggccg
     1981 caccgcgctc gacgaggtcc accatccctg caaccgcgtc cggatacttt cctgggcgtg
     2041 catcttcaag gtccgagaag tagatgccgg acgcgatcca ccagtccccg cccgcagggt
     2101 acgcataccc gatcttcggg acgtaggact cagccgagga ctcgtttatc ccgctggccg
     2161 aaggtgcggg gtagagatac gcgatgaacc ccccgccgtg tgctgccaca tgtgcaccta
     2221 tgcgtatggc aggcaggccg cgggcgtcag tccagtccac gcggttcgtc ccgacgcttt
     2281 cccgctggta ggggtgggca aggagcgtcc cgtgataatc gtaggcatac acatagaggt
     2341 cgccacgggt aaacgccccg gggttgcggt tgaattcgga gagcgcgggc tcttttccgg
     2401 catgaccggc atatgccgcc gcctcatgca cgaaaccggc aaggtcttca ggggtcattg
     2461 ccgcaggaat gtccggggga gattcagggg tggtgcaccc gcagaggagc aatgcggcgg
     2521 cgagtgagag gcaggccaga ccacagagca gccttggttg catgcgggcc ttatttggag
     2581 ggaactcaca taaatgcccc gatttatgtt tctttcccgc ggtgcaggag tcctacgcct
     2641 tctcttaaag aacaagcccg gtatggtgca gggtgccgac cggcaatcga ctgaacggat
     2701 atactggttc tgtcgtctgt aagagaagaa ggggacggtt cccagcgatt ggatgtgtcg
     2761 atggaaaaga tcagtcttcg cggatgtcct gtgtgccgtc ctcgatatcg tcatccggag
     2821 ctcgtgtatc cggcctgatg taccactcgg aacgacgcat cagcccgtgc agggtgcttg
     2881 cttcccgcgc cgtcaggttt gccctcccga ggatccttcg catcatcagc atcgtcccgt
     2941 ccctcttgaa atcggggtgg tcgatgaggg tgaggtactg gtcgatatgc tggtagaggt
     3001 gctccatctg ggtcctcccg gcgagtagat actctcccct gggaaggtcg gcaagctcgt
     3061 atgcgaggat cgccaccgcg tgggagaggt tcaggatggg gtaatcaggc gatgtgggga
     3121 tggtgcagat gacatccgag cggcggatct cgcggttgtt cagcccccag ttctccctcc
     3181 cgaagagaag agagaccttc ccgcggtgcg gttccacgag tgatcggatc tcccgggggg
     3241 agtagtaggg catccgcatc gggttgcaga tggacttgct cacctcgccg gtcgtggcga
     3301 tgacgaggtc gcttcccgag aagacctcgt cgagggtgag ccgggatgcg gactcgagca
     3361 cgtccttcgc atggcctgcg cgggcgatgg cgtcatcccc aagagggcag ggatcaacga
     3421 ggacgagccg tgagaacccg aagttcttga tggcgcgggc ggcaaatccc acgttcccct
     3481 cgtagagggg ctggaccagt acaaaatcga cctctggcat ccacggccta ctggacggct
     3541 ttgactgttg ccttcagcac gtcgatgccc tggtcgtaga tggcgttccc gactacgatg
     3601 gtgtctgcat acttcgccat ctccgaggcc cgctcggttg agttgatccc tcccccgtaa
     3661 tacagcactg atgaatcgag cgcctccgaa acggccctga ccacctcggg gtctccgtac
     3721 atgccgctgt actcgatgta cacgatgggg aaacggaagt agcgatcagc gactgtcgca
     3781 aaggcagcga cctccgcggg tgagagggaa cagttggagc gggtgacctt ccctaccgag
     3841 gagtcagggt tgaggacaat gtacgcctcg ggcaccacgc gctcccagtc gatctcatgc
     3901 tgctgcaccc actggtggtg cttgcccacg atccacctga cgtcctgggt gttcagcacg
     3961 cttgggatga agaggaagtc aatgccctcc gcgattactg cttccgggcc ggccggctcc
     4021 actacgaggg ggaggccgta tcttgccacc tgccgcagga gctcctgcat gttctccctg
     4081 gtcacgttca gcgtccccga gagcatcagg gcgtcggtcc cgctggtcac caccccggtg
     4141 atctgctcgg gggaaaggtg cttgtcgggg tcaagctttg tcacatggtt ccagctcttc
     4201 cagttgatgt gcatcacagg ccactccctc agatgacaat gatatcgtcg ctctgtttct
     4261 tcttcttttt gaagggtgct gctgcgcgga gttcccggac ccgctcgcgg ggaatgcccg
     4321 cccttgccga gagcgccgac tcgcctgtct cgtggagcga ctcgatattg tagactccgg
     4381 cccggagcag cgcttccagg gttgactcgg tgaccccctt gatcccgagg agttcggact
     4441 ttcccttctc gtattgggcc ctgctgactt tatcggggac gggcctgccg aggacggatg
     4501 ccaccgcctc gacgtgcctg aacacggtct caggagagat gccggtccga aaagagatgt
     4561 agagggggtg cgcggccagc atgtcctcgg gtcttgcgaa ccctgcctcc cggtacttct
     4621 tcagactgac ggcagggagg ccgatctcct tcaaccgtcg ctcgcctgcg actttctttg
     4681 cctctgactg gaggctctct gcctccttct cgcccacgcc cgcctggcgg agtactgacc
     4741 ggtcgacgag gccgagcccc tcgatgtcgt tgatgccgga ctcgtgcagc ttgcgaatga
     4801 cgctcgcggg gctcctgccc ttcctcggcg ggaggatgga ccgcacgaac ttccggcagt
     4861 ccgagcgccg ccgcaggagt tcgagagcat cggcggcctc tgcctgcagc ctgacgactt
     4921 cgtcacgcgc gatgccgagt gcgtgtgcga gcacttcggc cggggtgttc gcaaggtcgt
     4981 acacggtgta aatgtgggct gccaccatct tctggtggag ttcgtccgag aatgagggga
     5041 ggagcgcaag ggtctcttta tgggagctga tgtggtggca gagcgggcac ccaatgtccc
     5101 agggcctggc ccctttcctg acaaggcgga cgaagtggag ccggtgcgca gggcagacct
     5161 cgtcggtcct caccgcaaac ccccacatgg tcatggggag tccgatgttg aaactgcatg
     5221 cagggtaatg ggtgcagcct atgaactggg tctggttgcg gaggtgccgt atgcggaggt
     5281 cgtgggagca gaccgggcac ggcccaagcg tcagctcctc ggcggtctgg tccatgatct
     5341 cgtcgcctat ctcctggctg tgcgcctcga gctggtcaaa gacctggtgg agcatgcccc
     5401 gtgactccgc caccacgtca tccctggagc gggccttctg cttgatctgc tgcatgtgcg
     5461 cgtcaagggt ctgggtcatg tcggggcggg taatcgtgtc ggcgtggtgc tccaggctct
     5521 cgatgaccgc cctccccacg agtgtcgggc gcaggggatt tccctccacg tacttgcgcg
     5581 agacgagttt ctggatcacc tcgtgccggg tgctcttggt cccaaggcca agctcctcca
     5641 ttctctggat ca
//
LOCUS       DAOQYR010000120         5570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_14864_length_5570_cov_8.618314, whole genome shotgun sequence.
ACCESSION   DAOQYR010000120 DAOQYR010000000
VERSION     DAOQYR010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14864_length_5570_cov_8.618314"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PKM87_10705"
     CDS             <1..641
                     /locus_tag="PKM87_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315286.1"
                     /note="replaces guanine with 7-cyano-7-deazaguanine at
                     position 15 in the dihydrouridine loop of archaeal tRNAs;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-guanine(15) transglycosylase"
                     /protein_id="HNQ30578.1"
                     /translation="ELSELPCPCRICRSHSADELRESPEKERLLALHNLHVSLAEISR
                     IRQAIQDGVLWELVDERCRSHPALLRGYRALLSRAATLEEPDRVSKRRFFYRGSESCA
                     RTEVIRYHAMLERLTLPSTVLVSMDGRELPGFDLVLPFRPPFGPYPPELGETFPIGQS
                     EIPDWDEVMVAAGCWGIANLIETHPGCRVSISCPDKWTPVVRKEVPGVEVLP"
     gene            638..2278
                     /gene="arcS"
                     /locus_tag="PKM87_10710"
     CDS             638..2278
                     /gene="arcS"
                     /locus_tag="PKM87_10710"
                     /EC_number="2.6.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002948 - archaeosine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0002927 - archaeosine-tRNA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosine synthase subunit alpha"
                     /protein_id="HNQ30579.1"
                     /translation="MSTVEVRKRDGLARIATLIIGDKTCQLPTVLDTGILFDGLSGSR
                     MENVPLGADPAFVAQYLPRDRDFPAWVHPSGENIPGHGTCVMVPCWHTAFANPRRYVE
                     WLKAMKSALPPDTAWYAPGAALPSNAAILCYSGFDILDFTAVDLRSAQGRFCIPEGEF
                     PGDRIGSGVCTCPGCRSGDLKEHNRLALVRELALAREYIACQRLRDLVESRCRMDAAQ
                     VAILRHIDRARGFFEKAVPVARAGTLLATTGEVLQRPEVARFSERVVGRYRPPPADVA
                     VLLPCSARKPYSSSQSHRRFRQAIAGRALELIVTSPLGLVPRELELVYPAAHYDVPVT
                     GYWDAEEREFVGGIIGEFLEKHRFKRVIAHLEGGALAAAETGAARAGIGLEITCTGRP
                     TSHESLALLDSALSGENRVRHDAIRGTSSWQFDLAIDTRNLVIKGRYPWIRAFRGRTP
                     SFSIDEATGLLRPTFDGWNMIPEGYRVIIDEFVPTGDVLAPGVVDADPRIREGDEVLV
                     AGPCAIATGRAAMPADEMLRSGRGVAVRVRKVKTLPRT"
     gene            2321..3169
                     /locus_tag="PKM87_10715"
     CDS             2321..3169
                     /locus_tag="PKM87_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNQ30580.1"
                     /translation="MYRVYEAGQLADRIFQIWVEAPHVARHALAGQFLVIRIDEKGER
                     IPLTVSSVEGDLVRVIFMAVGKTTNQLSDLKAGDLIRDVAGPLGRPSEMGQYGTCVVV
                     GGGAGIASCPIIARAAREAGNHVVGIIGARNAGFLILEDEMRDACHELLVATDDGSRG
                     FHGFAADVLKQVIEQQQVDCVWIIGPAIMMKVTSEVTRPKGIRTFVSLNPIMVDGTGM
                     CGSCRVQVGGETKFACVDGPEFDAHLVDFDLLMQRQRTYFDEEKVSLERYHEHACRCG
                     EGSYHG"
     gene            3162..4517
                     /gene="gltA"
                     /locus_tag="PKM87_10720"
     CDS             3162..4517
                     /gene="gltA"
                     /locus_tag="PKM87_10720"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HNQ30581.1"
                     /translation="MGERPVRERLCDFLEVDCGLSPDEAVAEAERCLQCKKPSCVKGC
                     PVEIDIPAFIARIADRDFSAAARIIKDDNLLPAVCGRVCPQEVQCEGECILGVKDSPV
                     RIGALERFAADYEREQGFVVPPLAGPTGKLVAIVGSGPAGLTAAAELARAGHSVTVFE
                     SLHEAGGVLMYGIPAFRLPKDVVQAEIDQLRKMGVEIRTNHLAGASVPVPELLAYDAV
                     FIGTGAGLPFFMNIPGEQLNGVYSANEFLTRVNLMHAERFPEYDTPVRHAGRVVVVGG
                     GNVAMDAARVARRLGARVTLVYRRREEDLPARRAEVVRAREEGVEFALCANPVRVLGT
                     NFVTGVECVRMEMCEIDESGRPEPTPVGGSNFVIDADMVIQAIGQGPNPLLIRKLGGI
                     RYGRKGNIVVDEEGRSSLDRVYAAGDVATGAATVILAMGGAKKAARAIDRMLRDDRKG
                     H"
     gene            4683..5204
                     /locus_tag="PKM87_10725"
     CDS             4683..5204
                     /locus_tag="PKM87_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30582.1"
                     /translation="MGLMDLVWPVFFPALGAIAALVNIWWKRHSGASALRVFLMWQLA
                     IGLGLSYIYAGFGHLVFPDQVAESIGWTPGSPFQREVGMWDFSIGIVGLLCLKFRSIW
                     FWTAIVIAFGIFSIGAGIGHVYELVVHGDMSINNAGPVMYMDLLYPVFLGVLLFLCAR
                     RHDTFQSTRIEAD"
BASE COUNT         1048 a         1604 c         1818 g         1100 t
ORIGIN      
        1 aggagctttc tgagctcccc tgcccgtgcc ggatctgccg ctcgcactcg gccgacgagc
       61 tgcgtgaatc gcctgaaaag gaacgtctcc ttgccctcca taatctccat gtaagcctcg
      121 cggagatctc aaggatccgg caggccatcc aggacggcgt gctctgggaa ctggtggacg
      181 agcggtgcag gagccaccct gcgctcctgc gggggtatcg tgcccttctg agtcgtgcgg
      241 cgacactcga agagccggac cgggtgagca agcgaaggtt cttctaccgc gggtctgaaa
      301 gctgcgcaag gaccgaggtg atacgctacc atgcgatgct cgagcgcctc accctgccat
      361 cgacagtgct cgtatcgatg gacgggaggg aactcccggg ctttgacctc gttcttccat
      421 tcaggcctcc gttcggccca tatccccctg aactgggtga gacgtttccc atcggccaga
      481 gcgagatccc ggactgggac gaggtgatgg tcgcggccgg ctgctggggt attgcgaatc
      541 tgatcgagac ccacccgggc tgccgcgttt ccatctcgtg ccccgacaaa tggacacctg
      601 tagtcagaaa ggaggtcccg ggcgtggagg tgctcccgtg agcacggtcg aggtaaggaa
      661 aagagacggg cttgccagga ttgcaaccct cataattggg gacaagacct gtcaactccc
      721 aactgtcctc gacacgggga ttttgttcga tgggctgtcg ggctccagga tggagaacgt
      781 cccgctcggc gcagatccgg cgtttgtggc gcagtacctg cccagggacc gggacttccc
      841 cgcctgggtc catccctctg gagagaatat cccgggtcac gggacctgtg tcatggtccc
      901 gtgctggcac accgcttttg ccaatccgag gaggtacgtg gagtggctga aagcgatgaa
      961 atcagccctc cctccggaca ccgcctggta tgcacctgga gcggcgctcc cttcgaatgc
     1021 cgcaatcctc tgttactcgg gtttcgatat cctcgatttc accgcggtgg acctccgctc
     1081 tgcgcagggc aggttctgca tcccggaggg tgaattcccc ggggaccgga tcgggagcgg
     1141 cgtctgcacc tgccccgggt gccgaagcgg cgacctgaag gagcacaacc ggcttgccct
     1201 cgtaagggaa ctggcccttg cacgcgagta tatcgcatgc cagaggctcc gcgaccttgt
     1261 ggagagccgg tgcaggatgg acgccgcgca ggtggcaatt ctccggcaca tcgacagggc
     1321 tcgcgggttc tttgagaagg cagtccccgt cgcgcgggca ggaacactcc ttgcgacgac
     1381 gggcgaagta ctccagaggc ccgaggtggc caggttttct gaacgggtcg tgggccggta
     1441 ccggccgcct cccgctgacg ttgcagtcct cctcccctgc tcggcgagga agccctactc
     1501 ctcctcacag agccaccgga ggttcaggca ggcaatcgcc gggcgtgccc tggagctcat
     1561 tgtcacctcg cccctcgggc tcgtgccgag ggaactcgag ctggtctatc ctgcggcgca
     1621 ttatgatgtg ccggtcaccg gctactggga tgcagaggag agggagttcg tgggcggcat
     1681 cattggagaa ttcctggaga aacaccgctt caaaagggtc atcgcccacc tggaaggggg
     1741 agcgctcgcg gcggcagaga ccggtgcggc ccgggcaggc ataggacttg agatcacctg
     1801 cacgggcagg cccacgtccc acgaatcgct tgcactcctt gactctgccc tgtcagggga
     1861 gaacagggtt cgtcatgacg ccatccgggg gacatcttca tggcagttcg acctcgcgat
     1921 cgatacgagg aacctggtga tcaagggccg ctacccctgg atcagggcgt tcagagggag
     1981 gaccccctct ttctcaatcg atgaggcgac cggccttctc cgccccacct tcgacggctg
     2041 gaatatgatc cctgaagggt accgtgttat catcgacgag ttcgtcccta ccggggacgt
     2101 gcttgcgccc ggggtggtgg acgcagaccc gcgcatcagg gaaggggacg aggtgctcgt
     2161 ggccgggccc tgcgcaattg ccacgggaag ggctgccatg cctgcagacg agatgctccg
     2221 gtcagggcgg ggagtcgcag tcagggtgcg taaggtaaag acactcccgc gcacataact
     2281 attcgaaagc catctcgttg gcgttctcgg agtgaaacca ttgtaccggg tgtacgaggc
     2341 cgggcagctg gccgatagga tattccagat atgggtggag gccccccacg tggcccggca
     2401 cgcccttgca gggcagttcc ttgtgatccg catcgatgaa aagggcgagc gcatacccct
     2461 taccgtctct tctgtggaag gcgaccttgt ccgggtcatc ttcatggcgg tggggaagac
     2521 caccaaccag ctctctgatc tgaaggcagg agatctcata cgggatgtcg caggtccgct
     2581 gggaagaccg agcgagatgg gacagtacgg cacatgcgtg gtggttgggg gaggcgcagg
     2641 gatagcaagc tgcccaatca tcgcaagggc tgcacgggag gccggaaacc atgtcgtcgg
     2701 catcatcggg gccaggaacg ccgggttcct catactcgaa gacgagatgc gcgacgcctg
     2761 ccatgagctc ctcgtcgcca cggacgacgg gagccgcggg ttccatgggt ttgcggccga
     2821 cgtgctgaag caggtgatcg agcaacagca ggtcgactgt gtctggatca taggccctgc
     2881 gatcatgatg aaagtgactt cggaggtgac ccggccgaag gggatcagga cttttgtctc
     2941 cctcaacccg atcatggtgg acgggaccgg gatgtgcggt tcctgccgcg tccaggtggg
     3001 aggggagaca aagtttgcat gcgtcgacgg ccccgagttt gatgcccatc ttgtcgactt
     3061 cgacctcctg atgcagcggc agcggaccta ttttgacgag gagaaagtat ccctcgagcg
     3121 gtaccacgaa cacgcctgcc ggtgcgggga gggcagttac catgggtgaa cggcctgtcc
     3181 gggaacgact ctgtgacttt ttggaggtgg actgcggact ctcgcccgat gaagcggtcg
     3241 cggaggctga acggtgcctg cagtgcaaga agccctcctg cgtgaagggg tgcccggtgg
     3301 agatcgacat cccggccttc attgcccgca tcgcggaccg ggacttctcc gctgctgcac
     3361 ggatcatcaa ggatgacaac ctcctccctg ccgtctgtgg cagggtctgc ccgcaggaag
     3421 tccagtgcga gggggagtgc attctcggag tgaaggattc accggtccgc atcggggccc
     3481 ttgagcgctt cgctgcagac tatgagaggg agcaggggtt cgttgtcccc ccgctcgctg
     3541 gaccgaccgg aaaactggtg gcgatcgtgg gatctggtcc ggcaggactg actgccgcag
     3601 ccgagctggc gcgggccggg cactcggtga cggtctttga gtcgctccac gaggcgggag
     3661 gcgtgctgat gtatggaatc ccggcgttcc ggctcccaaa agacgtggtg caggccgaga
     3721 tagaccagct caggaagatg ggcgtcgaga tccgaaccaa ccacctggca ggggcgagtg
     3781 tcccggtgcc agaacttctc gcatacgatg cggtcttcat tggaaccggc gcaggacttc
     3841 cgttcttcat gaatatcccc ggtgaacagc tgaacggtgt ctactcggca aacgagttcc
     3901 tgacccgcgt gaacctgatg cacgccgagc ggtttcccga atacgacacc cctgtccgcc
     3961 acgccgggcg tgtcgtggtg gtcggcgggg ggaacgttgc catggacgca gcccgggtgg
     4021 cgcggaggct tggtgcacgg gtgacgcttg tctaccggcg gagagaggag gacctccccg
     4081 cacgcagggc agaggtggta cgtgcaaggg aagaaggagt ggagtttgcg ctctgtgcga
     4141 accccgtccg cgtcctcgga acaaattttg tgacgggtgt cgagtgcgtg cggatggaga
     4201 tgtgcgagat tgacgaatcc ggccggccgg aacccactcc tgttgggggg agcaacttcg
     4261 tgatcgatgc ggacatggtg atccaggcga tcggccaggg accaaacccc ctcctgatca
     4321 ggaagctcgg ggggatcagg tacgggagga agggaaatat tgtcgtcgac gaggaaggga
     4381 ggagctcgct tgaccgggtg tatgcagcag gggatgttgc caccggcgca gcgacggtca
     4441 tccttgccat gggtggggcc aagaaggcag cgagagcgat cgaccggatg ctccgggatg
     4501 atcgaaaggg ccattgatct cgtctgtcac acaccatccc atgccgacat cgcggtcctc
     4561 ctgggagaat tacgggacca gtcactaaaa cgaccttttt cccaattaca caaggggcgc
     4621 catatccctg attccaggag ggatacaatg tttttttatc gccctccatt caagtgatca
     4681 ccatgggact catggatctc gtatggccgg tctttttccc ggcgcttggc gcaatcgccg
     4741 ccctcgtgaa tatatggtgg aagcgtcatt cgggagcgtc tgccctccgt gtcttcctca
     4801 tgtggcagct cgccataggc ctcggcctct cttatattta tgcagggttt gggcacctgg
     4861 tgtttccgga ccaggttgcc gaatccatcg ggtggacccc tggcagcccc ttccagcgcg
     4921 aggtcgggat gtgggacttc tcgatcggga ttgtcgggtt actctgcctg aaattcaggt
     4981 ctatctggtt ctggactgcg attgtgatcg cgtttggaat attctcaatc ggggcaggca
     5041 tagggcatgt ctacgagctg gtcgtccacg gggacatgtc gatcaacaat gccggcccgg
     5101 taatgtacat ggatctcctg tatccggtct ttctgggcgt cctcctcttc ctgtgtgcca
     5161 ggaggcatga cactttccaa tccacccgga ttgaagcgga ctgatcagat gttgatgggg
     5221 ggattcacct acatttttcc tggcttttac tcaatcgaca gacagggtga gtggttcacc
     5281 cgacaacgct gaaagaaaaa aatgcgcatc ccgcatttta cttgcctcat cgctgttttg
     5341 tgtggggaga ctgaatcgag gttgttatct gcatatcaat tcagtcactc caaccgccgc
     5401 gggggcgccc cgtcgggggc ggcggcgtgc atcgcatatg ggggtgtggg gatggacacc
     5461 cctcaaaaat gtgtccggtt caccaatcct acccacacca gatagcgaag agccatttta
     5521 cttgcccatg aggggaatat tgcagtccgg gcaggtctgg ctcctgcagg
//
LOCUS       DAOQYR010000121         5545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_14933_length_5545_cov_7.807468, whole genome shotgun sequence.
ACCESSION   DAOQYR010000121 DAOQYR010000000
VERSION     DAOQYR010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14933_length_5545_cov_7.807468"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PKM87_10730"
     CDS             <1..360
                     /locus_tag="PKM87_10730"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HNQ30583.1"
                     /translation="ILVLALGILAFLKPDLVGGFLAVIAAALLIVGGLGMAVTGVFQG
                     PTFAGRVLSVIGGVLLAILGFLFLFHTGVTATLVVRLVGIFLLGAGAVFLAGAVLRWW
                     RERRCEPEWIDAEVVKR"
     gene            complement(385..1071)
                     /locus_tag="PKM87_10735"
     CDS             complement(385..1071)
                     /locus_tag="PKM87_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNQ30584.1"
                     /translation="MSRYSLTGNLKEAAFQRGADLFGVADASCFLSPDYQGNRPQEIM
                     PGVTSVVVLGVSVPRGAFSTLPKGRGEYTNTLMAGTATLRVIAFFLARQIEREGYLAT
                     IVPTEGSEFGYWYADRSTLKADLSLKYAAYHAGLGSFGRNHLLIHPDYGARVRMTGIL
                     TDAPLEAGSPGSAPFLHPQCEGCAKCAEICPAGAISRDGSIDRERCADYMFRELGGLR
                     CGMCIRVCPL"
     gene            complement(1084..2316)
                     /locus_tag="PKM87_10740"
     CDS             complement(1084..2316)
                     /locus_tag="PKM87_10740"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNQ30585.1"
                     /translation="MREFDAVQKEIKRLLGQIPDTERKKLAETLERLESRIHLLEHNY
                     NRAIRDRAAVHSLLNKSSNELIERYQTIFEFSGTAMAVIEEDGTISLVNTHFERLFGF
                     SREEVEWKKKFPEFLDESVKEYIVGFHRRRLSGDETVPHYYEAKIITRNGSTREVSFS
                     VGLFPGTGRIITSILDIEERKRAEKAVETAQKKIAILNSITRHDIHNQLTPLYLQIDM
                     ALMHTQDPVLRESLQKIERIGQNIQEQIEFMKLYQDVGVKSPIWQRVADVIRTATAGI
                     PLDGISQEIDVGELEIYADPLLQKVFYNLVENAIRHGGKLTRIGFSVKEDPNGVAIIC
                     VDNGAGVPAKEKEHIFERTFFKHTGFGLFLSREILSITGIGIRETGVPGEGARFEILV
                     PRGMFRVTLPNTLIERAP"
     gene            complement(2313..3455)
                     /locus_tag="PKM87_10745"
     CDS             complement(2313..3455)
                     /locus_tag="PKM87_10745"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNQ30586.1"
                     /translation="MIPEGRYFSAFSCEELAEKSREAMAVSPTLGIIFSSVHLGIPDV
                     ASSVNSLGIPIFGSSTAGEILGTGGEEPVQERTAACCFIPLDSTLFAVTLMERGDDSS
                     FELGARIGRWGREKFDDPAFIIAIAGLKNDGEAIVRGILSVSPPGTPLYGGLAGDDGR
                     MEETFVFSSAGYTSDGAVVMVLDRSRVDLSGIVTSGWTGVGMPMTVTSSEGNVLHTLN
                     NRPATKVLQEYLNAGEDDLVETAIMFPLLVQRPDGTVVLRTCLSADFSRGSMIFAGSI
                     PRGAQVRFSSSFGYETIEKSIRDITSFHATRPDADLVIAFSCMARHWAAGTMVGDEIA
                     AALRLWKKPLVGFFTYGEIGQGHTGQCDFHNESLCLVLLTVNPPTP"
     gene            complement(3686..4054)
                     /locus_tag="PKM87_10750"
     CDS             complement(3686..4054)
                     /locus_tag="PKM87_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30587.1"
                     /translation="MSWQDLALTSFIFLAGVLLIPQLLDTMNRGAVVNFFSASLTSVL
                     LFCISSVFASLGLWISVIAQSFVAVVWVCLAFFSLRNVRNSQFPDKSLFFVARDFLGV
                     WIFGVTFLVSNGARRLLRRD"
     gene            complement(4383..5027)
                     /locus_tag="PKM87_10755"
     CDS             complement(4383..5027)
                     /locus_tag="PKM87_10755"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HNQ30588.1"
                     /translation="MTPIPWETPQDPLTQSSAGAMTGSAGEIRRPCSMAGAFLFSASV
                     LFMLGEVVLVAWYPGYLITENFISDLGVGPTATGFSVMLITCGVLIMAASWLLHTHST
                     SQSIPLLLFASGAGGAGVGIFPETLGAIHFICAGIAFLSGGVAAVLLSSRLHGPFRLF
                     SAILGVLSLFFIVSHILHLYFSLGPGGSERLIAYPLFLWMAGFGGHLMADRRAS"
     gene            complement(5024..>5545)
                     /locus_tag="PKM87_10760"
     CDS             complement(5024..>5545)
                     /locus_tag="PKM87_10760"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNQ30589.1"
                     /translation="LLGSHVNGKPNFMALGWFSRVNANPPLIGCGVGNHHATTPGIRE
                     SRTFSINVPDASMIREVDYCGLVSGSNVDKSALFSVFYGETGTTPMIRECPLCLECRL
                     VGSMQHTTNEFFVGEIVASWTSERFMTEGKLDIRKMNPLLLTMPDNRYWTVGEEVGAA
                     WSAGRDLARYGEK"
BASE COUNT         1128 a         1634 c         1622 g         1161 t
ORIGIN      
        1 atcctggtcc tcgcgctcgg gatactcgca tttctcaagc ctgacctggt cggcggattc
       61 cttgcagtga tcgccgctgc cctcctgatc gtgggaggcc tcgggatggc cgtgaccggg
      121 gtgttccagg ggcccacctt tgccgggcgg gtgctctccg tgatcggcgg cgtgctcctt
      181 gcaatcctcg ggttcctctt cctcttccac accggggtta ccgcgaccct tgtcgtccgg
      241 ctggtgggaa tctttctcct cggtgcaggg gcagtcttcc tcgcaggcgc cgttctccgg
      301 tggtggcgag agaggaggtg cgaaccggag tggatcgacg cagaagtcgt aaagagatga
      361 tccggcccgt cggggtcttt ttgatcagag cgggcagacc ctgatgcaca tcccgcaccg
      421 gagccccccg agttcccgga acatataatc ggcgcaccgc tcgcggtcga tcgagccgtc
      481 ccgggagatt gcgcctgcag ggcagatctc cgcacatttc gcgcacccct cgcactgcgg
      541 gtggaggaac ggagccgacc ccggagatcc tgcctcgagc ggcgcatcgg tgaggatgcc
      601 ggtcatccgg acccgggcgc cgtagtccgg gtggataagg aggtggtttc tgccgaacga
      661 gccgagccct gcgtggtacg cggcatactt aagggagagg tcggctttca gggttgagcg
      721 gtccgcgtac cagtacccaa actcgctccc ctcggtaggg acaatggtcg caaggtatcc
      781 ctcccgttcg atctgccgtg cgaggaagaa ggcgatcacc cggagcgtcg cagtcccggc
      841 catcagggtg ttcgtgtact cccccctccc cttcgggagc gtcgagaagg ccccgcgggg
      901 gaccgagacc ccgagcacga cgactgaagt gacgcctggc attatctcct gcgggcggtt
      961 cccctggtag tcgggggaga gaaaacatga cgcgtccgcc accccgaaga ggtctgcgcc
     1021 ccgctggaaa gccgcttctt tcaggttgcc ggtcagggag tatctgctca ttgtgccaga
     1081 tcgctatggg gctctttcta ttagggtgtt tggcagggtg accctgaaca tcccgcgggg
     1141 gacgaggatc tcgaaccggg cgccttctcc cggcacccct gtctccctga tcccgatccc
     1201 ggtgatggag aggatctccc ggctgagaaa gagcccgaac cccgtgtgct tgaaaaaggt
     1261 cctctcaaag atgtgctcct tctctttggc cgggacccct gccccgttat cgacgcagat
     1321 gatggcaacc ccgttcggat cctccttgac agagaaccca atacgagtca gtttcccccc
     1381 atgcctgatg gcgttttcca ccaggttgta gaagactttc tggaggagag gatctgcata
     1441 aatctcaagt tcgcccacgt caatctcctg ggatatcccg tcaaggggaa ttcccgcggt
     1501 agcggtgcgg atgacatctg ccacccgctg ccatattggg gattttacgc caacatcctg
     1561 gtaaagtttc atgaactcta tctgttcctg gatgttctgc ccgatgcgct cgatcttctg
     1621 gagagattct ctcaataccg ggtcttgtgt atgcatcagg gccatatcga tctgaaggta
     1681 aagaggggtc agctggttgt ggatatcgtg ccgtgtgatc gaattgagga tggcaatctt
     1741 cttctgtgca gtctctactg ccttctcggc cctttttcgc tcctcaatgt caaggatgga
     1801 cgtgatgatc ctccctgttc ctgggaagag gccgacgctg aaggagactt ccctggtgct
     1861 gccgttcctt gtaatgatct tggcctcgta gtagtggggg acggtctcgt ctcctgaaag
     1921 gcgcctccgg tggaacccga cgatatactc tttcacgctc tcgtcaagga attccgggaa
     1981 tttcttcttc cactccactt cctcgcgtga gaacccgaac agtcgttcga agtgagtgtt
     2041 tacgagcgag atggtcccat cctcctcgat cactgccatc gcggttcccg agaactcaaa
     2101 aattgtctga taccgctcga tcagctcgtt tgaactcttg ttgaggagag agtgaacggc
     2161 tgcacgatca cggatcgccc ggttgtagtt atgctccaaa agatggatgc ggctctccag
     2221 ccgttccagg gtttcggcga gcttctttct ctcggtatca ggtatctgac caaggagccg
     2281 cttaatctct ttttgcaccg catcgaattc tctcatggcg tgggaggatt cactgtgagc
     2341 agcaccaggc agagactctc gttatgaaaa tcacactgtc cggtgtggcc ctggccgatc
     2401 tcgccgtagg tgaaaaaccc cacgagcggc ttcttccaca agcgcagtgc cgcagcgatc
     2461 tcgtctccca ccatggtacc tgctgcccag tgcctggcca tgcaggagaa cgcgattaca
     2521 aggtctgcat ccggccgggt ggcatggaaa gacgtgatat ccctgattga tttctcaatc
     2581 gtttcgtatc cgaatgaact cgagaaccgt acctgggctc ccctggggat tgaacctgca
     2641 aatatcattg acccccggga aaagtcggcc gagaggcagg tgcgcaggac cacggtgccg
     2701 tccggcctct ggaccaggag gggaaacatg atcgcggttt ccacgaggtc atcttcgccg
     2761 gcattcagat attcctggag gacctttgtc gccgggcggt tgttcagggt gtggaggaca
     2821 ttgccttcag acgatgtgac ggtcatcggc atgccgaccc ccgtccaacc gctggtgacg
     2881 atgccggaca ggtccacccg ggatcggtcg aggaccatga ccactgcgcc atccgatgta
     2941 tagccagccg aagagaagac gaaggtctcc tccatccttc catcatcccc cgcaagaccc
     3001 ccatagaggg gagtgccggg tggagaaacg gagagtatcc ccctgacaat ggcttccccg
     3061 tcgtttttca gtcccgcaat cgcaatgatg aacgccgggt cgtcgaattt ctcacggccc
     3121 catcgaccga tccttgcccc aagttcgaaa ctggagtcgt caccccgctc cattaaggtg
     3181 acggcaaaga gggtggaatc caggggaatg aagcaacacg cagctgtccg ctcctgtacg
     3241 ggctcctcac cacctgtgcc gaggatctcg ccggctgtgg agctcccaaa tattggtatt
     3301 ccaagggaat tgacggaact tgcgacatcg gggataccga gatgcaccga ggagaagatg
     3361 ataccaagtg tcggagagac agccattgcc tccctgctct tttcagccag ctcctcgcag
     3421 gagaacgccg agaaatatct gccctccggt atcatgtgcg tctcctttta tttctatctt
     3481 aagacctctt aaatggtgag ggattgcgcg ttcaaagatt cggaatgcca gccccagggc
     3541 ttcttcgcat ggttgcgaag gcacaggagg ggtgcttctt ccctgtctcg catgcgtggc
     3601 attcggattc gcaccttccc ccctcttctg cagcaagcgc ccgcctgttt aaaacaaaag
     3661 cccgggcctg tttcccggtc ccggatcagt cccttctcag gagcctccgg gcgccgtttg
     3721 agaccaggaa tgtcacaccg aatatccaga caccaaggaa atccctagcc acaaagaaga
     3781 gggatttgtc ggggaactgt gaattccgga catttctaag ggagaagaac gcaaggcaca
     3841 cccagacgac cgcgacaaac gactgtgcaa tgacggatat ccagaggcca agcgatgcaa
     3901 agacagaact gatgcagaac aggagcacgc tcgtcaggct tgcggagaag aaattgacca
     3961 ctgcgccacg gttcatcgtg tccagcagtt gcgggatgag cagtactccc gcgaggaaaa
     4021 tgaagctcgt gagtgccagg tcctgccagc tcataaggaa ctcctgtgat gtcccatttt
     4081 gcagaataag catcgacgtc aatgcatatc aaaagttaca tcctcctttt ccctgaaaga
     4141 tgcgcgtcca gcggcaaaga ggggttatca ggctacgcga tagtcacgcc tgcctaccca
     4201 gtagtgaggc atgtcgcccg gtcttttaag gggcgttccc gagtggtgct cagttcctcc
     4261 tcatctgccg gagtggggga ataatccgca cgatagtgag gcttcccgcc ccctggaggc
     4321 cgtagccgat ccccttggac cccggtgccc tgccacccgg tggtgccatg cgtggttcct
     4381 ggtcaggaag ccctgcgatc ggccatgagg tgccctccaa acccggccat ccagaggaag
     4441 agcggatagg cgatgagccg ttcggagccc ccggggccga gcgagaagta gaggtggagg
     4501 atgtgggaga cgatgaagaa gagagagagt acgccgagga tggcggagaa cagcctgaat
     4561 ggcccatgca ggcgggatga gagcagcacc gccgcaactc cccctgacag gaatgcgatt
     4621 cctgcacaga tgaagtggat agcgcccagg gtttcgggga atatccctac acccgctccg
     4681 cctgcaccgg aggcaaagag aaggagaggg atgctctggg atgtagagtg agtatggagc
     4741 agccaggagg cagccatgat caatactccg caagtgatta gcatcaccga aaagccggtt
     4801 gctgtcggcc ccactccgag gtcactaatg aagttctccg tgataaggta cccgggatac
     4861 catgcaacca ggaccacctc gccgagcatg aacagaaccg aggctgagaa gaggaatgct
     4921 ccggccatgg aacagggcct gcggatctcg ccggcactcc cggtcatcgc acctgccgaa
     4981 gactgcgtga gagggtcctg gggggtctcc caggggatgg gtgtcatttc tcaccatacc
     5041 gtgccaggtc tcttccggca ctccatgccg ctccgacctc ttcgccgact gtccagtacc
     5101 ggttgtctgg catggtcagg aggaggggat tcatcttcct gatatccagt ttcccttccg
     5161 tcatgaaccg ctcgcttgtc caggacgcaa cgatctcccc cacgaagaat tcgtttgtcg
     5221 tgtgctgcat ggaaccgacc agccggcatt cgaggcagag ggggcattcc cggatcatcg
     5281 gggtggtccc ggtctcccca tagaagaccg agaagagcgc cgacttgtct acgttgcttc
     5341 ccgacacaag cccgcagtaa tccacctccc tgatcatcga tgcatccggg acgttgatgc
     5401 tgaaagtccg actctccctg atgccaggcg tggtcgcgtg gtggttcccc accccgcacc
     5461 cgatcagagg gggattggcg tttacccggg agaaccaccc gagcgccatg aaattgggct
     5521 tcccgttgac gtgtgaccca aggag
//
LOCUS       DAOQYR010000122         5474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_15165_length_5474_cov_8.311128, whole genome shotgun sequence.
ACCESSION   DAOQYR010000122 DAOQYR010000000
VERSION     DAOQYR010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15165_length_5474_cov_8.311128"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..887
                     /locus_tag="PKM87_10765"
     CDS             63..887
                     /locus_tag="PKM87_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HNQ30590.1"
                     /translation="MVNFGGFVDLSTIDWPGRAVCTVFLRGCPLRCSYCHNAAIQSGS
                     DMRDIDEVVAKIDAARPLVSGVIFSGGEPTLQREPLLALARAAKERGLQVGIQTNGFF
                     PDTLEALINERLVDRVALDYKSRWEGFAKRWEGYESVSTRDYTSQVQRSMALCQEAHL
                     KGALAEYEVVLTIFWGNEKEILAIEKDLPRGTIVLQQGVHKRFWKDWTLSKEVHGKSY
                     RSDEEVRGDRAPLTYDELAALAAKMVRVGRTIKIRTRDLGEVIYEGDRGRWVSGKR"
     gene            847..1395
                     /locus_tag="PKM87_10770"
     CDS             847..1395
                     /locus_tag="PKM87_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ30591.1"
                     /translation="MKVIGVVGFPASGKGEFSRIAAEEGVPIVVMGDVIREAVQEEGL
                     PVTDANLGAVANRLRREEGMDAIARRSLPRIRACRAPLVLVDGIRGDAEVALFRKEFP
                     GFFLVGIDAPFDERLTRLSMRGREDDFSQSAKLVERDGREVGWGLGRALSSADYTLSN
                     AGTLEEFSRAARTLLCRIMEDP"
     gene            1392..2165
                     /locus_tag="PKM87_10775"
     CDS             1392..2165
                     /locus_tag="PKM87_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNQ30592.1"
                     /translation="MKNPVSFSASSKVWEDITWIYGIEEAGFSGWEIVADGNYRLDSP
                     ENVRRINEAISSTHLSITVHAPYADLNLSTLNYPIWRESVRQVCTCIEQASAWTDRVT
                     IHPGYLSPVGKIMPQRAWDQQKQALREIGRVAADHGSIACLENMIGIREFLCRDPGEL
                     FGMTEGIEGIGVTIDIGHAHTVGLVKEFLPHLGKASHMHIHDNCGQHDEHLAIGEGTI
                     DWTAVGSAIVSRYKGILVVEGRSLEEGKKSLRVVNGWGA"
     gene            2162..3130
                     /gene="rnz"
                     /locus_tag="PKM87_10780"
     CDS             2162..3130
                     /gene="rnz"
                     /locus_tag="PKM87_10780"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNQ30593.1"
                     /translation="MSGETLHVYFLGTAAALPTPMRNPACIMIKRGRDTLLFDCGEGA
                     QQQMMRARTGFLVDAVFITHWHADHFLGLFGLAQTLSFMGRTEPLTIYGPEWVHEVAA
                     SVRALSRYNVKFPVNSVEIRPGSVVRFSGYTVRAFETAHGMAGLGYVLEEDERPGRFN
                     RQRAIELGVPPGPLFGRLQRGETVRIVKDGGEREVKPGDVMGQPRPGRKIVYTGDTRP
                     IHTRIRELAHDPDLLIHDATYDDQAIDRAREVLHSTAGEAGEAAEFLNARRLALVHIS
                     SRYPTTAGHLKDASARFGGQVLVPSDLEMLEIPYSDSGEATAGSGD"
     gene            3227..5071
                     /locus_tag="PKM87_10785"
     CDS             3227..5071
                     /locus_tag="PKM87_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNQ30594.1"
                     /translation="MNDTNNLVYRMGPGCDLGDVEVGKTYIGRVQGFANFGTFVQLND
                     RVKGLVHKSNARTTHRERESILVRVTNIRSNGNIDLEEVTIPVYTVEMVEKKSTAVRL
                     SELGGKVGRTVRIEGEIAQVKQTSGPTIFTIVDETGTENAAAFVEAGVRAYPEAELGD
                     IVRIVGEVMMRQNQLQIEVESLSILAGEEAEAVRERIDRALESRAEPPDIPFLVASDV
                     LEKLRPEMRKVAKVIRKAVFSSQPILLRHHADADGICSGVAIEQAVVSLIRESGGDFD
                     ADYFLFKRAPSKAPFYEIEDITRDLDFATKDHARYGQKMPLVILTDNGSTEEDLPSMK
                     IAQVYDLPIVVIDHHHPDAIVDQYIAGHVNPYHVGGDFGITAGMLGTEVARMINPSVE
                     NLIRHLPAVAAVGDRSEAPERARYLSLVQDAYSEQDCKDIALALDYQQFWLRFNDGRE
                     LVKDLLNLAGNTSRHKKLVALQVEGANLAIQDQMSACMPHVVHRTLRNGAELFLLDVE
                     IHAHKFTFPPPGKTSGEVHDRLCQQHAGSPVVTIGFGPDFAVLRSRGVMMNIPKMVRE
                     LRDEIPGGGISGGGHLVVGSIKFVEGMREEVLGGLIDKISIVQAGMPG"
     gene            5257..>5474
                     /locus_tag="PKM87_10790"
     CDS             5257..>5474
                     /locus_tag="PKM87_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30595.1"
                     /translation="MNASRNIRDKYNETQTRIVQYLKGGLEKGKHYFKSKYIAKELGL
                     SPKEVGTNMAILSEICHDLAIIKWSYSN"
BASE COUNT         1119 a         1613 c         1756 g          986 t
ORIGIN      
        1 ctgctccttc catagcttga agaaacttcc gcgcgcaata taagaggacg gtgattcggg
       61 tgatggtgaa tttcggcggg ttcgtggacc tgagcacgat agactggccg gggagggcgg
      121 tgtgcacggt ttttcttcgc ggctgcccgc tccggtgctc gtactgccac aacgccgcca
      181 tccagtcagg gagcgacatg cgggatatcg acgaggtggt cgcaaagata gatgcggccc
      241 gcccgctcgt ctcgggcgtg atcttttcag gcggtgagcc cacgctgcag agagaacccc
      301 ttcttgcgct cgcccgggcc gcgaaggaga gggggctcca ggttggcatc cagaccaacg
      361 ggttctttcc cgataccctg gaggcgctga tcaacgagcg gctcgtagac cgcgtggcgc
      421 tcgactacaa gagccggtgg gagggctttg ccaagcggtg ggaagggtac gagagcgtca
      481 gcaccaggga ctacacttcc caggtgcagc gttcaatggc cctctgccag gaggcccatc
      541 tcaagggagc tcttgccgag tacgaggtcg tcctgaccat cttctggggc aacgagaagg
      601 agatcctcgc gatcgagaag gatctcccga gggggaccat tgtcctccag cagggggtgc
      661 acaagcggtt ctggaaggac tggacactct cgaaggaggt gcatggaaag tcctaccgct
      721 cggacgagga ggtgagaggg gaccgcgccc cgctcaccta cgatgaactg gcggcacttg
      781 cggcgaagat ggtgcgggtg ggcaggacca taaagatacg cacaagggat ttgggggagg
      841 tgatctatga aggtgatcgg ggtcgttggg tttccggcaa gcggtaaggg cgagttctcc
      901 cgcatcgccg cggaggaggg ggtccccatc gtggtgatgg gcgatgtcat acgagaggcg
      961 gtgcaggagg aaggattgcc ggtcacggac gcaaacctcg gcgcggtggc aaaccgcctc
     1021 cggcgggagg aggggatgga cgccatcgca cgccgctcgc tcccccgcat ccgtgcctgc
     1081 agggcgccgc ttgtcctcgt cgatgggatc aggggggatg cagaggtcgc gctcttcagg
     1141 aaggagttcc cggggttctt tctggtcggg atcgatgccc cgttcgacga acgcctcacg
     1201 cgcctctcca tgcggggcag ggaggatgat ttctcgcagt ctgcgaagct cgtggagcgc
     1261 gacgggcggg aagtcggctg gggccttggg cgggcgctct cttcggcgga ctacactctc
     1321 tcgaacgccg ggacactgga agagttctcc cgggctgcac gaacccttct ttgcaggatc
     1381 atggaggacc catgaagaac cctgtctcct tctctgcctc gtcaaaggtc tgggaggaca
     1441 tcacctggat ttatggcatt gaggaggccg ggttttcagg ctgggagatc gtcgccgacg
     1501 gaaactaccg cctcgactcc cctgagaacg tgcgcagaat caatgaggcc atctcctcca
     1561 cgcacctctc catcacggtc cacgcaccct acgccgacct gaacctctcg accctcaact
     1621 accccatctg gcgggagtcg gtcaggcagg tgtgcacctg tatcgagcag gcctcggcat
     1681 ggaccgaccg cgtaaccatc cacccgggat acctctcgcc ggtgggaaag atcatgccgc
     1741 agcgcgcgtg ggaccagcag aaacaggccc tccgggagat cgggcgcgtc gcggcagacc
     1801 atgggtcgat cgcctgcctc gagaacatga tcgggatcag ggagttcctc tgccgcgacc
     1861 cgggcgagct cttcgggatg accgagggga tcgaggggat aggcgtcacc atcgatatcg
     1921 ggcatgcaca cactgtgggt ctcgtgaaag agttcctccc gcacctcggg aaggcctcgc
     1981 acatgcatat ccatgacaac tgcggccagc atgacgagca cctcgccata ggggagggga
     2041 ccatcgactg gacagcagtg gggtcagcca tcgtctcgcg ctacaagggc atccttgtcg
     2101 tggaggggag gagcctggag gaaggcaaaa agagcctccg ggtcgtgaac gggtggggag
     2161 catgagcggc gagacgctcc acgtctattt cctcgggacg gccgcggcgc ttcctacccc
     2221 catgcgcaat cccgcctgta tcatgatcaa gcggggccgg gacaccctcc tctttgactg
     2281 cggggagggt gcccagcagc agatgatgag ggccaggacc ggtttcctgg tggacgcagt
     2341 cttcatcacc cactggcacg cggaccattt cctcgggttg ttcgggctcg cccagaccct
     2401 ctcgttcatg ggcaggaccg agcccctgac catctatggc cccgaatggg tccacgaggt
     2461 cgcagcgtct gtccgggcgc tctcgaggta caacgtgaaa ttccctgtca attctgtcga
     2521 aatccggccc ggttccgtcg tgcggttctc aggctacacg gtccgggcat tcgagacagc
     2581 ccacggcatg gcggggctcg gctacgtcct cgaggaggac gagaggccag ggcggttcaa
     2641 ccgacagagg gccatcgagc tcggggttcc ccccgggccc ctctttggcc ggctgcaacg
     2701 aggggagacc gtccggattg tgaaggatgg gggtgagaga gaagtgaagc ccggcgatgt
     2761 catgggtcag ccgcggcctg ggcgaaagat cgtctatacc ggtgacaccc ggccaatcca
     2821 cacccggatc agggagctcg cgcacgatcc ggacctcctc atccacgatg ccacctacga
     2881 cgaccaggcc atcgaccggg cgcgggaggt gcttcactcc actgcgggag aagcgggaga
     2941 ggcggcggaa ttcctcaatg cccgccggct tgcgcttgtc catatcagct cgaggtatcc
     3001 caccactgcc ggccacctga aggatgcatc cgcacggttt ggcggccagg tgctggtccc
     3061 ttccgatctc gagatgctgg agattcccta ctccgattca ggggaagcaa ccgctggctc
     3121 cggggattaa ttccatactt acataagaat cccgcgccta ttgatatact caaaggagaa
     3181 cggatcggga ggggggaaga agatcctgcc tcgatctggc atacctatga atgacacgaa
     3241 taatctggtg taccgaatgg ggccggggtg cgacctcggc gacgtggaag tcgggaagac
     3301 gtatataggc cgcgtccagg ggtttgcaaa tttcggcaca tttgtacagt tgaacgaccg
     3361 ggtgaaggga ctggtgcaca agagcaatgc acgcaccacc caccgggaac gggagagcat
     3421 cctcgtcagg gtgacaaaca tcaggagcaa cgggaacatc gatctcgagg aggtcacgat
     3481 cccggtctac accgtcgaga tggtggagaa gaagtcgacc gcggtccgtc tctcggagct
     3541 cgggggaaag gtgggaagaa cagtccgcat tgagggggag atcgcgcagg tcaagcagac
     3601 gagcggcccc accatcttca ccatcgtgga cgagaccggg accgagaacg cggcggcttt
     3661 cgtcgaagcg ggcgttcggg cctatcccga ggctgaactc ggggacatcg tcaggatcgt
     3721 cggcgaggtg atgatgcggc agaaccagct ccagatcgag gtggagagcc tctcgatcct
     3781 cgccggcgag gaggccgagg ccgtcaggga gcggatcgac agggcgctcg aatcacgtgc
     3841 cgagccaccc gacatcccgt tccttgtcgc atccgatgtg ctcgagaagc tgcggccgga
     3901 gatgcggaag gtggcaaagg tcatcaggaa agcggtcttc tcctcccagc caatcctcct
     3961 ccggcaccat gcggacgcgg acgggatctg ctcgggagtc gccatcgagc aggcggtggt
     4021 ctccctcata cgggagtcgg gcggtgactt cgacgccgat tacttcctgt tcaagcgtgc
     4081 cccctcgaaa gcgccgttct acgagataga ggacatcacc cgcgacctcg acttcgcgac
     4141 gaaggaccac gcccggtacg ggcagaagat gccgctcgtg atcctcaccg acaacggctc
     4201 caccgaggag gacctcccat cgatgaagat cgcgcaggta tacgatcttc ccattgtcgt
     4261 gatcgaccac caccaccccg atgcaatcgt ggaccagtac atcgccgggc acgtcaaccc
     4321 ctaccatgtg gggggcgact tcgggatcac ggccgggatg ctcggaaccg aggtggcgcg
     4381 gatgatcaac ccgtcggtcg agaaccttat ccggcacctc ccggccgtgg cggcggtcgg
     4441 cgacaggagc gaggcgccgg agcgtgcccg ctacctctca cttgtccagg acgcgtactc
     4501 ggagcaggac tgcaaggaca tcgcgctcgc gcttgattac cagcagttct ggctccggtt
     4561 caacgatggc cgggagcttg taaaagacct cctgaacctt gcagggaaca cctcgcggca
     4621 caagaaactc gtggccctcc aggtcgaggg agcaaacctt gccatccagg accagatgag
     4681 tgcctgcatg ccccacgtcg tgcaccggac gcttcggaac ggggcagaac tcttccttct
     4741 cgatgtcgag atccatgcac acaagttcac cttcccgccc cccggcaaga catcaggcga
     4801 ggtgcacgac cgcctctgcc agcagcatgc cgggtcgcct gtcgtgacga tcggctttgg
     4861 acccgatttc gccgtgctcc gttcacgtgg cgtcatgatg aacatcccga agatggtgag
     4921 ggagctccgg gacgagatcc ccggcggcgg catctctgga ggcgggcacc ttgtcgtagg
     4981 gagcatcaag ttcgtggaag ggatgcgtga ggaggtctta ggtggcctca tcgataagat
     5041 ctccattgtc caggccggga tgccaggttg agcccctcct gttcttttaa ttaccatccc
     5101 ctgcaatctt tcccttaggt aagtgtcgag aattccttta gataaagccg aattcgctga
     5161 aagttcgagt ataaactctt tctggatgcc acggcgaata gatatatatt tatagatgca
     5221 taagtgtacc attctctcac ggctgaggtg atactcgtga acgcatcacg aaatatccga
     5281 gataagtata atgaaactca gacccggatt gtccagtacc tcaagggagg tctcgagaag
     5341 gggaaacact acttcaagtc caaatatatc gccaaggaac ttggtctctc cccgaaggag
     5401 gtggggacca acatggccat cctctccgag atctgccacg acctggcaat catcaagtgg
     5461 agctattcaa acag
//
LOCUS       DAOQYR010000123         5371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_15562_length_5371_cov_8.584086, whole genome shotgun sequence.
ACCESSION   DAOQYR010000123 DAOQYR010000000
VERSION     DAOQYR010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15562_length_5371_cov_8.584086"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PKM87_10795"
     CDS             <1..390
                     /locus_tag="PKM87_10795"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNQ30596.1"
                     /translation="EEFLESHPEVAAILDEIPKEGVIAVILFGSHATKKAGPLSDIDL
                     CIVGDRAFTEHQKNELLSFGSRIIELSLFWDLPLTIRFRIIRDGIILYCESPELLRRI
                     TVDTVRDYLDVEPLIRKHSLAVIRTRG"
     gene            723..1994
                     /locus_tag="PKM87_10800"
     CDS             723..1994
                     /locus_tag="PKM87_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNQ30597.1"
                     /translation="MRPAGVALVLALLLLFCYAAAGAELLWTEKVNGEYITGIAVSED
                     GSRVVVGTSMGGLYLYDGEGTLCWSERHKGTMQVGISPDASLVVAGESESREKDKGAL
                     RAYDTNGTLAWMRHTGWICGFGTSEDLGRIAVGNRLGQVAVYDREGFEEAWEDNLLKR
                     YYATSAAGLSPDGRMLAYSLLEHTPAIYLLNVDTWRTRTIRSVFREHGSPVHTLVLSG
                     NGSSLLAASGEGSTDTVYLFTDSGALKWKETVPDILDMEISSDSALILLGSEDGCIRA
                     YNPAGDLTWTRCMEGAVQSLSLTPEGDLLAAGSRGGEILLLSGSGELRWEHRLVRFPA
                     ARIDALEISTQGNALVAVVNRNEVLFFSLGPDTLDSQNSPPEPVAGPLPSTEPTTLET
                     PVPPSLWGIPRAGSGFSEYGSALRAIFRISQ"
     gene            2088..3284
                     /locus_tag="PKM87_10805"
     CDS             2088..3284
                     /locus_tag="PKM87_10805"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNQ30598.1"
                     /translation="MHARAFLLTCVMAVLVTLGAAASAAVITVSPDHVNPGDTITVTV
                     QGLADESSLKMSWEVYIEAPGPTFLWSVEGLSFPINLQDASFRLTNQNTVTNKVTLQN
                     EIPNYETRELIHSGNSVDGTWTVYHGNDFINGTWPVIRNEGTVEEGVTHVLSLVEWYG
                     IKRANPEIPEQENGGPEDFMIPFSFSGFEDGRVKFTIQVNGTDVLTDEVTVGSPSSRT
                     GTLYLKSSPTSAQVYIDGIYYGLSPRKVTGVSPGIRTVKMTKEGYVDYVEQLTVSAKG
                     LTLVMATLNPATASIKVSSIPSHAEVYLDSALVGTTPMTITGVGPGTHTVALMKEGYQ
                     DYTRTVTITDGETVRLINIFLLKSGFSQFPGMNYPNWSAGGPGSRIDPGAIIERVNAR
                     FDKVRF"
     gene            3370..4152
                     /locus_tag="PKM87_10810"
     CDS             3370..4152
                     /locus_tag="PKM87_10810"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ30599.1"
                     /translation="MPATTRTTAAACLLLFIIAAALCAVPASAEMVIVSGDAHSTAAA
                     WNDMGNGYANQYRWDAAIDAYTRAISLDPAYARAYFNRGRAYAALKQYDQAIADYEHA
                     IAIDPSQGPVVAPYLESALAVRYPTIPSGSLVKGYWQTGGQFLAIDNSQGASDLVVAL
                     APRGSRGALLAVYVAKGYSHRFDQVVPQGPYDVYITFGERWDPVTKSFVKVGGYLKWE
                     LPQYFGGAGTQGYTMTFITWQPPGSWWSYTLTPIDRQQFPVL"
     gene            complement(4481..4912)
                     /locus_tag="PKM87_10815"
     CDS             complement(4481..4912)
                     /locus_tag="PKM87_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNQ30600.1"
                     /translation="MALTSQQEAEICGMMDCYAEMYRTRNAKGLFSLFSPGICGYGSG
                     PDEVVKDAAAMKAQVKRDLLQADSVRIRFDIQRVSGEMPVAWVMADCDFEVVVKGTSL
                     CMTGRMTAVLRNTGSRWLFEMVHFAVPAMEQAPGESYPGSA"
     gene            complement(4985..>5371)
                     /locus_tag="PKM87_10820"
     CDS             complement(4985..>5371)
                     /locus_tag="PKM87_10820"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNQ30601.1"
                     /translation="SVNVQSDATTRISQTLSVGPTPTPLPGTGSIAVHSTPAGAEVYL
                     DNAYVGISPLTIPSVSPGSHVVLLTMPGYANWQATVQVQRDVIASVDATLSPSPTSAP
                     TKSGLPAALAVLGVGLLAVAFMAKRR"
BASE COUNT         1100 a         1632 c         1624 g         1015 t
ORIGIN      
        1 gaggagttcc tggaatcgca tcctgaagtc gccgcgatac tcgacgagat cccaaaagaa
       61 ggggtgatcg ccgtcattct ctttgggagc catgccacaa aaaaggccgg gcccctctcg
      121 gatatcgacc tctgtatcgt tggagatcgg gcatttaccg agcatcaaaa gaacgagttg
      181 ctcagttttg gatcacgtat cattgaattg agtctcttct gggatctccc gctcactata
      241 aggttcagga tcattcggga tggaataatc ctctattgtg aaagcccgga attactccgc
      301 cgtattactg tggatactgt ccgggattac ctcgatgtag aaccgctcat aagaaagcac
      361 agcctggcag tgatccggac ccgggggtga cctggcagat gccggtatct tacgacataa
      421 tccgaatcgg gtcaatttta aaggacatta cccggtattt caaggatctt gaaagcctaa
      481 aaatccagga tgtgtgtgat ctctctgatt cacggaattt ttatgaagct tcgatgattc
      541 ttttcgcgcc gaaagaagga tgtcgtcgcc ggaaagggag ggcaggtggc tccgcatgag
      601 tccccccagg aatgcatgat cgtttccagg aaacgggggc tgcggtacgc agccggccac
      661 gcctgccagg cgggaataaa tctggaacat tattaggcct tgcgggagaa ccaaagacag
      721 gcatgagacc cgcaggtgtt gcactggtgc ttgcacttct cctcctcttc tgttatgcag
      781 ccgcgggggc ggaactgctc tggacggaga aagtgaacgg cgagtacatc accggcatcg
      841 ccgtcagcga agacggttcc cgggtcgttg tcgggacctc catggggggc ctgtacctct
      901 acgacgggga agggacgctc tgctggtcgg agcggcacaa ggggaccatg caggtcggga
      961 tctctcccga cgcgtccctc gtggtcgccg gagaatcgga gtcgagggag aaggacaagg
     1021 gcgctcttcg ggcatacgac acaaacggga ccctcgcctg gatgaggcat accggctgga
     1081 tctgcgggtt cgggacctcg gaagacctgg gccggatcgc agtcgggaac cgcctgggcc
     1141 aggttgcggt ctacgaccgc gagggattcg aggaggcctg ggaggataac ctcctgaagc
     1201 gttactacgc caccagcgca gcagggctct cccccgacgg gagaatgctc gcctactccc
     1261 tcctcgagca cacccctgcc atctacctgc tcaacgtgga tacgtggagg acacgcacca
     1321 taaggtcggt cttcagggag catgggagcc ccgtgcacac cctggtcctc tcagggaacg
     1381 gcagctccct gctggctgca agcggtgagg ggagcaccga cacggtgtat ctcttcacgg
     1441 acagcggggc actgaaatgg aaggagaccg tcccggacat ccttgacatg gagatctctt
     1501 cggatagcgc actgatcctg ctcgggtctg aagacggctg catccgcgcc tacaatcccg
     1561 cgggagacct tacctggacc cgctgcatgg agggggcggt gcagtcgctc tccctcaccc
     1621 cggagggaga cctgctggca gccgggtcga gggggggaga gatcctcctc ctctccggca
     1681 gcggggagct gcggtgggaa caccggcttg tgcggtttcc cgccgcacgc atcgatgccc
     1741 ttgagatctc cacccaggga aatgcgcttg tggcggtggt aaacagaaac gaggtgctct
     1801 tcttctccct cgggccagac accctcgatt cccaaaactc tccccccgaa cccgttgccg
     1861 gacccctccc ttcaacggaa ccgaccaccc tcgaaacccc cgtccctcct tccctctggg
     1921 gcataccgcg cgccgggtcc gggttcagcg agtatgggag cgcactgcgg gcgatattca
     1981 ggataagcca gtgacagaat tccttcgcgc ccggaatcgc cgaggggttc cggtaacctt
     2041 atcttgccac cacaaacagg taattgtata agaagcctgt gatatgcatg cacgcccgcg
     2101 catttcttct cacctgtgtg atggccgtgc tggtgaccct tggggccgcc gcctcggccg
     2161 cagtcatcac tgtctcccct gaccacgtga accccgggga taccatcacc gtgacggtcc
     2221 aggggctcgc cgacgaatcc tcgcttaaga tgagctggga ggtctacatc gaggcgcccg
     2281 gccccacctt cctctggagc gtggaggggc tttcgttccc catcaacctg caggacgcct
     2341 cgttccggct caccaaccag aatacggtga ccaacaaggt cacgctccag aacgaaatcc
     2401 ccaattacga gacccgggaa ctgatccatt ccggcaactc ggtggatggg acctggacgg
     2461 tataccatgg aaatgatttc atcaacggca cctggccggt gatcaggaac gagggcacgg
     2521 ttgaggaggg agtgacccac gtcctctcgc tcgttgaatg gtacgggatc aagcgggcca
     2581 accccgagat ccccgaacag gagaacggag gccctgagga ctttatgatc ccgttctcct
     2641 tctcgggatt cgaggacggc cgggtcaagt tcaccatcca ggtgaacggg accgatgtcc
     2701 tcaccgacga ggtgacggtg ggatcaccct cctcacggac agggacgctc tacctcaagt
     2761 caagccccac ctcggcccag gtctacatcg acgggatata ttatggtctt tcgccccgga
     2821 aggtcaccgg cgtttcgccc ggcatccgca cggtaaagat gacgaaggag gggtacgtgg
     2881 actatgtcga gcagctcacc gtctccgcaa aggggctcac actggtcatg gcaaccctca
     2941 acccggcgac ggcgtccatc aaggtgagtt ccatcccctc gcatgcggag gtctacctcg
     3001 attcggcgct cgtggggacg acaccgatga caatcaccgg tgtgggcccc gggacccaca
     3061 ccgtcgcgtt gatgaaggaa gggtaccagg actataccag gaccgtcacc atcaccgatg
     3121 gagagacggt tcgcttgatc aatatcttcc tcctgaagag cgggttctcc cagttcccgg
     3181 ggatgaacta cccgaactgg tcggcaggag ggcctggatc gcggatcgac cctggtgcaa
     3241 tcattgagcg ggtcaacgca cggttcgaca aggttaggtt ctgatacccc ctcctctttt
     3301 ctcatcctgc ctctctgtgt acgagggtat ataaaggcca tccgtatatt cctttacagg
     3361 agatacccta tgcctgccac cacacgaacc accgccgcag cctgcctgct cctgttcatc
     3421 attgcagcgg ctctgtgtgc agtgcccgca tccgccgaga tggtgatcgt ctcgggggac
     3481 gcccactcca ccgccgccgc atggaacgac atgggcaacg ggtacgccaa ccagtaccgc
     3541 tgggacgcgg cgatcgatgc ctacacccgg gcgatatccc tggacccggc atatgccagg
     3601 gcgtacttta accggggcag ggcgtatgcg gccctgaaac aatacgacca ggccatcgcc
     3661 gactacgagc atgccattgc gatcgaccct tcccagggcc cggtcgtggc accctacctt
     3721 gagagcgcgc ttgcggtccg ctaccccact attccgagcg gatcgcttgt aaagggatac
     3781 tggcagaccg gcggccagtt cctcgccatc gacaactcgc agggcgcaag cgacctcgtc
     3841 gtggctctcg ccccccgcgg ttcaaggggc gcgctccttg ccgtctacgt ggcgaagggg
     3901 tattcgcacc ggttcgacca ggtcgttccc caggggccct acgatgtcta catcaccttc
     3961 ggcgagcggt gggaccccgt gacaaagtca tttgtgaagg tgggcgggta cctgaagtgg
     4021 gagctcccgc agtacttcgg gggcgcagga actcaagggt acaccatgac cttcatcacc
     4081 tggcagcccc cgggttcctg gtggagctac accctcaccc ctatcgaccg ccagcagttc
     4141 ccggtgctgt aggatctggc accacttttt tcccggggtc acctggctgc agccaaggag
     4201 ccggtaagaa tccgtctgcc caaggtccac tcccatgaac gatgtccctt ctccggatga
     4261 atgcctgcgg agggcgggaa ccgccagatg tcccgataag agtgagggga aataaatcct
     4321 gcggggatcc cgttcccaag tacgtcgggc aggcttgaga gcgtccatgt caggcagggc
     4381 gaatgagccg gcacagggga aagggccgat ttcgttcaaa gaaaataacc ggccatccct
     4441 gccacttcag gagagttacg ggaaaagatg tggggatatt ctacgcactc ccgggatacg
     4501 actcgcccgg tgcctgctcc atggcaggga ccgcgaaatg caccatctcg aagagccagc
     4561 gggagccggt gttcctgagc accgcggtca tgcggccggt catgcagagg gaagtgcctt
     4621 tcaccaccac ctcgaaatcg cagtcggcca tcacccacgc gaccggcatc tctccagaga
     4681 cccgctggat atcgaaccgg atacggaccg agtcggcctg gagcaggtcc cgcttcacct
     4741 gcgccttcat cgcagcggcg tccttcacta cttcgtcagg gccgctcccg tacccgcaga
     4801 tgcccgggga gaagagcgag aagagtcctt ttgcgttcct ggtgcgatac atctctgcgt
     4861 aacagtccat cataccgcag atctcggctt cctgctggct ggtgagtgcc atggatggca
     4921 ggttgcacgg gataggatga aagcgtttct ctttgcgcaa gggaaaaaag aggggaagtt
     4981 ctcttcagcg cctctttgcc atgaacgcca cggcaaggag gccgaccccg aggaccgcga
     5041 gtgcggccgg gaggcctgac ttggtcggcg ccgaggtcgg tgacggggag agtgtcgcat
     5101 caaccgaagc gatcacgtcg cgctgcacct ggaccgtggc ctgccagttg gcgtaaccag
     5161 gcatcgtaag caggaccacg tgggatcccg gtgagaccga ggggatcgtg aggggggaga
     5221 ttccgacata ggcgttgtca aggtaaacct ctgcacctgc cggagtagaa tggaccgcga
     5281 ttgagccggt ccccggcaga ggagtcgggg tcgggccgac cgagagggtc tggctgatcc
     5341 gcgtggtcgc gtccgactgc acgttcacgg a
//
LOCUS       DAOQYR010000124         5354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_15633_length_5354_cov_8.233818, whole genome shotgun sequence.
ACCESSION   DAOQYR010000124 DAOQYR010000000
VERSION     DAOQYR010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15633_length_5354_cov_8.233818"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..922
                     /locus_tag="PKM87_10825"
     CDS             146..922
                     /locus_tag="PKM87_10825"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNQ30602.1"
                     /translation="MNESIGCIGGGRIVHILLHGWSEAGLLPADIIVSDPNKGALDLI
                     AKEFPGVRVVQDDNSLAAGQDLVLIAVHPPDAGKVLGAIAPALKPGATVISLMPKLTI
                     AQLSGMLGGFARIVRMIPNAPSMVLSGYTPVTFSEGVAPADRAMVGTLLESLGHYFEV
                     PEQDLEAYAVISAMGPTYLWFQLYELEQIAVSFGLSPERAADAVRKMAEGTVQTMMWS
                     DLTPGQVMDLVPVRPLGEDEPAIREMYRRRLPTLYQKLKG"
     gene            929..1345
                     /locus_tag="PKM87_10830"
     CDS             929..1345
                     /locus_tag="PKM87_10830"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HNQ30603.1"
                     /translation="MAEPDFVRESSMDLDDRPVLLLTCSGISHTGQLTTMAGNSLRCR
                     QPILIARHVRLTSLTRPLEKELTGDECLIAVDGCEECCAKKRMDLIGKIPDAYLIATR
                     EGITKRGMEEPRYDEIECLCQAVVQTIRNGGRTNEE"
     gene            1335..1814
                     /locus_tag="PKM87_10835"
     CDS             1335..1814
                     /locus_tag="PKM87_10835"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HNQ30604.1"
                     /translation="MKNDNLKRKIVVLGVLVLLVTLAVLGAGCTAPAATNESQITTTP
                     GTVQKLEVYHFHRTQQCYSCRTVGAYAEETVKTYFAPELESGRVVFGHINIEEPENKA
                     LVDQYGPTGSSLWIGVYDKDGFHKEENVNVWYKIGDKEAYLAYLKEVVDKRLAGDLS"
     gene            1823..2542
                     /locus_tag="PKM87_10840"
     CDS             1823..2542
                     /locus_tag="PKM87_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HNQ30605.1"
                     /translation="MGSMEALGTSGIPLVAAFFIGLVMTFSPCPLATNITAIAFISKK
                     IGDNRHTLLVGTLYALGRMVAYIGLTALIVYAGLNIQSISLFLQEYGEKLIGPFLVIM
                     GILMLEIVDIPSIGGQGWLQKIELYLGEKGYLGGFLLGVIFALALCPFAAVLFFGMLI
                     PVALKTGDAIFVPAVFGIATALPVIIISIVLVSGVNRVSGMMTAVQKNEKWIKWAVAA
                     VFIIVGVYYIVIVYGPMVGIV"
     gene            complement(2735..3127)
                     /gene="hgcB"
                     /locus_tag="PKM87_10845"
     CDS             complement(2735..3127)
                     /gene="hgcB"
                     /locus_tag="PKM87_10845"
                     /inference="COORDINATES: protein motif:HMM:NF040864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation ferredoxin HgcB"
                     /protein_id="HNQ30606.1"
                     /translation="MFDSYRENTLLYYPERCINCRRCSQVCPHGVFAEGSETAVLTDS
                     TRCMECGACALNCPVQAIEVQSGVGCAWAMISAALRGKDMDNCTCGGEAGSCCSDTTQ
                     TSSCCGDSAKTSPCCPDSAVKSSCCDEK"
     gene            complement(3127..4095)
                     /gene="hgcA"
                     /locus_tag="PKM87_10850"
     CDS             complement(3127..4095)
                     /gene="hgcA"
                     /locus_tag="PKM87_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation corrinoid protein HgcA"
                     /protein_id="HNQ30607.1"
                     /translation="MTTVSTLTFADRFDHFLARWGVNRMSHLVKPGLYRLGNPTPDSP
                     VFASANYTLSFDALRSALIGIDAYILVLDTKGINVWCAAGKGTFGTDELVNRISCTGL
                     ASVVRHRTVIVPQLGAPGISAHQVLRRSGFRVEYGPVRARDLPEYLKTRKATTDMRRV
                     RFPFVDRVVLALVELVHAAVPTLIAAILLYFLAGPVASLAAVTAVLAGTVLFPALLPF
                     LPTRDFSTKGLILGVLVALPFAVYGATQFHLFPFAHVVAGIIPLLLMPPVVAYLALNF
                     TGCTPHTSRTGVKQEIFRYVRVMVAMAGCGIFLAILSAIVWFVGVA"
     gene            complement(4214..4498)
                     /locus_tag="PKM87_10855"
     CDS             complement(4214..4498)
                     /locus_tag="PKM87_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HgcAB-associated protein"
                     /protein_id="HNQ30608.1"
                     /translation="MVKKKSPGGSGSPGGCVPGTCCNVEAVLSVDERGQMVLPKEVRQ
                     KAGIGTGDKLALISWEREGEVCCLALMKVSNLSGMVQGILTPLVQDMEKE"
     gene            4754..4924
                     /locus_tag="PKM87_10860"
     CDS             4754..4924
                     /locus_tag="PKM87_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30609.1"
                     /translation="MHLGNTDVTMLLIGAFLVFIGIGFVFALDALWPYAIAGFLILVG
                     IWVLIKGFYHIG"
     gene            4961..>5354
                     /locus_tag="PKM87_10865"
     CDS             4961..>5354
                     /locus_tag="PKM87_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30610.1"
                     /translation="MIEALVATLLPLGFLIVLFGGGAHFLRNNIEQDGEAPINRTLFY
                     VSKYSILVLWGAMILQIWGIGISLYEVPPLFQVIGLLSWCFGFALLFIGRFEMGGSFR
                     LGTPKEHTSLKVDGLFRLSRNPMYVGMYA"
BASE COUNT         1208 a         1458 c         1524 g         1164 t
ORIGIN      
        1 gcgattgatt atggggggaa ccggtacgag gcacttaccc ccgatctgct gaagcaggtg
       61 attatcgggg tcgttgaatc gtgggagact ccgtgcagct gtggctgcga ttgcgacccg
      121 gatatgaaat gcgagtgaac ctgtcatgaa tgaatcaatc ggatgtatcg gcggaggacg
      181 gattgtccat atcctcctgc acggatggag tgaagccggg ctgctgcctg ctgacatcat
      241 tgtcagtgac ccgaataagg gggcacttga cctgattgca aaggagttcc ccggcgtgag
      301 ggttgtacag gacgacaata gtcttgccgc cggacaggac cttgttctca ttgcagtcca
      361 ccccccggac gcagggaagg tgctcggcgc gattgctccg gcactcaagc ccggagccac
      421 cgtcatctcg ctcatgccca agctgaccat cgcccagctc tccgggatgc tcgggggatt
      481 cgcccggatc gtccggatga tcccgaatgc accgtccatg gtactgagcg ggtatactcc
      541 ggttaccttc tcggaggggg tcgcaccagc ggaccgtgcg atggtgggaa cactcctgga
      601 atccctcggg cattatttcg aagtccccga gcaggacctg gaggcctatg ccgtgatttc
      661 cgccatgggg ccgacctacc tctggttcca gctctatgaa ctcgagcaga tcgcggtctc
      721 gttcgggctc tccccggaac gggctgccga tgcagtccgg aagatggcag aagggaccgt
      781 gcagaccatg atgtggtccg atctcacccc cgggcaggtt atggacctcg tcccggtccg
      841 gcctctcggc gaggatgaac cggcgatcag agagatgtac cggagacgcc tcccaaccct
      901 ttaccagaaa ctgaagggct gaaccactgt ggctgagcca gacttcgtca gggaatccag
      961 catggacctc gatgaccggc cggtcctcct ccttacctgc tccgggatct cgcataccgg
     1021 gcagctcact acgatggcag ggaattccct ccggtgccgg caacccatcc ttatcgcccg
     1081 tcatgtgagg ctcacttccc tcacgcgacc cctggagaag gaattgaccg gggatgaatg
     1141 cctcatcgcg gtggacgggt gcgaggaatg ctgtgcaaag aagaggatgg acctgatcgg
     1201 gaagatcccg gatgcctatc tcatcgcgac aagagaaggg atcaccaagc gagggatgga
     1261 agagccgcgc tacgatgaga tcgaatgcct ctgccaggct gttgtccaga ccattaggaa
     1321 tggtggaaga acaaatgaag aatgataatt tgaagcgaaa aattgtagtc ttgggagtgc
     1381 tcgtactgct cgtaaccctt gccgtcctgg gagcaggatg caccgctccg gccgcgacga
     1441 acgaaagcca gataacgacc actcccggga ctgtccagaa attggaagtc taccatttcc
     1501 accggaccca gcaatgctat tcctgtcgaa ctgtcggggc atatgccgag gagaccgtga
     1561 agacctactt cgccccggaa ctggaatccg ggagggtggt attcggccac atcaatatcg
     1621 aagagccgga gaacaaggcg ctggtggacc agtacgggcc gacaggatcc tcgctctgga
     1681 tcggtgtcta cgacaaggac gggttccata aagaggagaa cgtcaatgtc tggtacaaga
     1741 tcggggacaa ggaggcgtat ctcgcctact tgaaggaggt cgtcgacaaa cgactggcgg
     1801 gtgacctctc ctgatcgatt tcatgggcag catggaagcg ctggggacca gtggtatccc
     1861 cctggtcgca gcattcttca tcggactggt gatgaccttc tcgccctgcc ctcttgccac
     1921 caacatcacg gctatcgcat tcatctcaaa gaagatcggg gacaacagac acacgctcct
     1981 cgtaggtacc ctctatgccc tggggcggat ggttgcctac atcggtctca ccgccctgat
     2041 cgtctatgca ggactgaata tccagtccat ctctctcttc ctccaggaat acggggagaa
     2101 gctgatcggg ccgttcctcg tcatcatggg gatcctgatg ctggagattg tggacatccc
     2161 ctccatcgga ggccagggat ggctgcagaa gatcgagctc tatcttgggg aaaagggata
     2221 cctcgggggc ttccttctcg gggtcatctt cgcccttgcc ctctgcccgt tcgccgcggt
     2281 cctgttcttc gggatgctca tcccggttgc cctgaagacc ggggatgcga tcttcgtccc
     2341 ggcggtcttt ggcatcgcga ccgcccttcc ggtcatcatc atctcaatcg tgctcgtctc
     2401 gggagtgaac cgggtttccg ggatgatgac tgcggtacag aagaacgaga agtggatcaa
     2461 gtgggcagtc gctgccgtgt tcatcatcgt cggagtgtac tatatcgtca tcgtatacgg
     2521 gccgatggtt gggattgtgt aggtatagag aaaatttaaa aaaagaccac aggattctct
     2581 ttcgacacac atgtgcacaa aatgaaaatc gctgatgtga ttttcgtata aaccgccaat
     2641 gactgctcaa cagtcgatga taaaatatga aaacgccgaa ggcgttttca ttgaaaaaga
     2701 agtgcgaatc atccgtggaa tttggactag tacgtcactt ctcatcgcaa cacgacgatt
     2761 tcactgcact gtcgggacaa cagggcgacg ttttcgcact atctccacaa caggatgacg
     2821 tttgagtagt atcggaacaa caggaacccg cctcaccccc gcaggtgcaa ttgtccatgt
     2881 cttttcccct gagggccgca ctgatcatcg cccaggcaca gccgacccca ctctgcacct
     2941 cgatcgcctg gaccgggcag ttgagggcac aggctccgca ctccatacac cgggttgaat
     3001 cagtgagtac tgccgtctct gacccttccg cgaacacgcc gtgagggcat acctggctgc
     3061 atcgccggca attgatacat cgctccggat agtacagaag agtattctca cgatacgagt
     3121 cgaacatcag gcaaccccca cgaaccagac gatggcggag agaatggcaa ggaagatccc
     3181 gcaccctgcc atggcaacca tcacccggac atacctgaat atctcttgtt tcaccccggt
     3241 ccgcgacgta tggggggtgc agcctgtgaa gttgagtgcc aggtacgcta ccacaggtgg
     3301 cattaggagg aggggaatga tcccggctac gacatgtgcg aagggaaaca ggtgaaattg
     3361 tgttgcaccg tacaccgcaa acgggagtgc gaccagtacc ccgagaatta atcccttcgt
     3421 gctgaagtcc ctggttggga ggaacgggag gagcgctgga aagagcacgg ttcctgccag
     3481 gactgccgtg actgcagcga gggatgcaac agggcctgcc aggaaataca ggagtatcgc
     3541 agctataagg gtcggaactg cagcatgtac gagctcaacg agagcgagca caacccggtc
     3601 gacgaaggga aaccggaccc gccgcatatc agtagttgcc tttctcgtct tcaggtattc
     3661 cgggagatcc cgggcacgga ccggtccgta ctccacccgg aacccggatc tccgcaggac
     3721 ctgatgggcc gagatgcccg gagcgccgag ctgaggcacg atcacggtcc ggtggcgtac
     3781 cactgatgcc agccccgtgc aggatatcct gttcacgagt tcatcggtcc cgaacgtacc
     3841 tttgccggca gcacaccaga cattaatgcc tttggtgtca agaacaagga tgtaggcatc
     3901 aatcccaatg agggccgacc ggagtgcatc gaagctgagc gtgtagttcg ccgatgcgaa
     3961 aaccggtgag tccggtgtcg gattgccgag acggtagagt ccgggtttta ccaggtgact
     4021 catccggttc acaccccacc gtgccaggaa atggtcaaac ctgtcagcga aggtaagcgt
     4081 gcttaccgtg gtcatcgctt ccgctggttg tgcaggtgtt attgcaccgc agcagggaac
     4141 atcatcctgt tgttgggcgc tggaacagca taagcacgat tgagtatcag gcgaggattg
     4201 attcaatctg ttctcactcc ttctccatat cctgcacgag gggagtgagg ataccctgga
     4261 ccattcccga caggttgctg actttcatga gcgcaaggca gcagacctct ccctcacgtt
     4321 cccagctgat caatgcgagc ttgtcaccgg ttccaatccc ggctttctgc cggacttcct
     4381 tgggaagcac catctggccc cgctcatcga cactcagcac cgcttccacg ttgcaacacg
     4441 taccggggac acatccaccg ggtgatcccg atccaccggg gcttttcttc ttcaccatag
     4501 cgcagataat acttcatctt ccatatatta ataattttca gataaatcag aaaaatcatt
     4561 gattgtgctg atcccggaag agtgttcagg acaatgctcc ttgcataaga gaagttaaaa
     4621 gagtccgggc cgcaaccaag gtgctcctga tcatcattcc tggtagtgct aatcaactac
     4681 aaagggcagg tgtccctggt gaagatatct caagggagtg gaagtctgga actgttgaaa
     4741 tgagagtgaa ggtatgcacc tggggaacac cgacgtgacg atgcttctca tcggagcttt
     4801 cctggttttc attgggatcg gattcgtctt tgcgctcgat gcactctggc cctatgccat
     4861 tgccggattt ttaatcctag ttggtatctg ggtcctgatt aagggattct atcatatcgg
     4921 atagggaaag gacgggagaa caaattcctg ggtgactggt atgatcgagg ctcttgtggc
     4981 aactctcctt ccccttggtt tcctgattgt gttattcggc ggaggggcgc actttttgcg
     5041 gaacaacatt gaacaggacg gggaggctcc gataaaccgg actctgtttt acgtcagtaa
     5101 atactcgatc cttgttctgt ggggggcgat gatcctccag atctggggga tcggcatatc
     5161 attgtatgaa gtgccccctc tcttccaggt aatcggcctg ttatcgtggt gtttcggctt
     5221 tgcgcttcta ttcataggaa gattcgaaat gggggggtca ttccgcctgg gcaccccgaa
     5281 ggaacacacc agtctcaaag tcgatggatt attcagattg agcagaaatc cgatgtatgt
     5341 cggtatgtat gcaa
//
LOCUS       DAOQYR010000125         5309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_15810_length_5309_cov_8.905025, whole genome shotgun sequence.
ACCESSION   DAOQYR010000125 DAOQYR010000000
VERSION     DAOQYR010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15810_length_5309_cov_8.905025"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..294
                     /locus_tag="PKM87_10870"
     CDS             67..294
                     /locus_tag="PKM87_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30611.1"
                     /translation="MDPEEKAMLADLIWLNAVIATELIQITENTSAILRKEPPPQSCL
                     EDHRRLKSIATTIAERYHPGSGLKEHLVGHQ"
     gene            281..1060
                     /locus_tag="PKM87_10875"
     CDS             281..1060
                     /locus_tag="PKM87_10875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ30612.1"
                     /translation="MVTSEPDAGTVRPPQGENGIQANDPCPADACSVDPPYTLIIPAY
                     NEETRIPRLLSQLGGARGEIICICEGDDHTPALVSEFAQNHRGVDIRCDRRAVRLGKG
                     GAILEGMRQARAPLVGYMDADASTSFSQMLALFALLDGADAVIGSRWIEGSVLVRGQG
                     LFRRLESRAFNGIIRTLFGLPFKDTQCGAKVFKKAAIDAVIEQMVSTGFEFDVELLWR
                     IRNCGFRIREHPITWNDMGDSRVRGNDVVRMLASLLRLRFS"
     gene            1070..2887
                     /locus_tag="PKM87_10880"
     CDS             1070..2887
                     /locus_tag="PKM87_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30613.1"
                     /translation="MPLQTADERKRLAFQRFEQGDFPGSYALCRDLVAGSADPAVAIL
                     CATNLFQMGRLEEAEAYFRDLANSIPESSHVHSYLGRILEKKGDDQALSEFAHAVALD
                     PGNLEALRSYASFLLATGDPGRAVPVLRQLAARSRRPDDSLLLVRALLLNGMPREALD
                     VRRGPLCRGIPDDDEYIQILDACGEYAETATASRAAFERTGNLGYARSALLALARFNP
                     GAAPEEYQKFLSEKDDPGIRFDYVRLLKERGEWRMALRELGLLLTGKRVEPMFLLEEC
                     EILWGLGERESASLHFTRLIDRELEGLADPEFLAMLLSRYRAFLQTHYPVREAESLLL
                     ERLATQPHVICLLCMASFYEDIGDHTEARSWYYRAYRSDFFYGGPEYARFCEKEGDVR
                     ECEKVMLYVINSIRKTGDLVRVAGMVMEDTHALYRMPRLMERLRERLEEKASGLSSRG
                     LELLALSLLVSASHCLERGDYARCKRSCLEGLDFIPPGSHDIHAEDFLVLIDECKRQS
                     LCDLPVLVYAPLPGDLKDPGSELPEIHLDLDENEKKIVDFLRVHQQATETELRMLLGT
                     RRAGGMVNRIIQKASSAGIVVIEKRGVGKDGEIYAFKKS"
     gene            2868..4172
                     /gene="brxD"
                     /locus_tag="PKM87_10885"
     CDS             2868..4172
                     /gene="brxD"
                     /locus_tag="PKM87_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding protein BrxD"
                     /protein_id="HNQ30614.1"
                     /translation="MHSRSPDIQPADRLESQNIINALRRGTVPASGLERIAVGLDIEE
                     DVVAAQLDFVARGGGDIKFIRGDYGSGKTFLVARALEIAREKGFVTAHVVISSSSPLH
                     KTKGIYQQICASLRTREEEHAQKSIIDSWLFGIEERILKVSDRTLSEEVLEKATLAEI
                     ETALAGISELNTSLAAALRTYYRANNAGDFQNSQAAIGWICAEPNVGREFKQKAGIRG
                     DVDEATAFTFLRGLLRIIVSAGYSGLSVAIDEVETTQSLPRNQREKGYRTLQMIVDSL
                     DRGDLPNSYFIFTGTPAFFDGPRGIRTLPPLYDRISVIQTGEGFRNPRQPQITLAKFD
                     AKKLEQVALKVMDIYSAAYTEVDRSRVSHRFIRAMIKKVTGKFGGRVDVIPRIFLKEY
                     VDVLDKCELYEDFDPWEQYHFETDHLKGELKEEEEAVMVVEF"
     gene            4286..4813
                     /locus_tag="PKM87_10890"
     CDS             4286..4813
                     /locus_tag="PKM87_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30615.1"
                     /translation="MSVHLAPFLFVAGCLMLLAGFASGGITLDAQPHTPFIGERVHIS
                     GISDIPNTIAVYLFVTGPGLNRAGVTLENLHLTAGSGFFTSAFVHPDGRFEYEWNTGF
                     VVGQLIPATYRIYVVNVPLNLDRLTDRDEISLSHIDVTFSRPPSPEIGIPWGIPALSL
                     AFAAAVLFSWRRKRG"
BASE COUNT         1164 a         1513 c         1526 g         1106 t
ORIGIN      
        1 tgaagaacat ggccctgccc attggacccg ccatgattat tcgtatgccc gccaatacac
       61 tcttccatgg acccggaaga gaaggcaatg cttgccgacc ttatttggtt gaacgcagta
      121 attgcaacgg agctcatcca gatcaccgag aacacctctg ccatcctgag aaaggagcct
      181 cccccacagt catgcctgga ggaccacagg aggctcaaaa gcattgcgac cacgatagca
      241 gagcggtatc accccgggtc gggactgaag gagcaccttg ttggtcacca gtgagcctga
      301 tgcggggacg gtgcggcccc cccaggggga gaacggtatc caggcgaacg atccgtgccc
      361 ggcagatgca tgcagcgttg acccccccta cacgctgatc atccctgcgt acaacgagga
      421 gactcgtatc ccacgactcc tctcgcaact tggaggagca cgcggcgaga tcatctgcat
      481 ctgcgagggc gatgaccaca cacctgcgct cgtatcggag ttcgcgcaaa accatcgcgg
      541 cgtcgacatc cggtgcgacc gcagggccgt gaggcttgga aaaggtggag cgatcctcga
      601 ggggatgcgg caggcccgcg ccccccttgt gggttacatg gacgcggatg catccacctc
      661 attttcccag atgctcgcgc tcttcgcact tcttgacggg gctgatgcgg tgatagggtc
      721 acggtggatc gagggatcgg tcctcgtccg cgggcagggt ctcttcagga ggctcgagag
      781 cagggcattc aacgggatca tccgcacact cttcggcctc cctttcaaag atacgcagtg
      841 cggggcaaaa gtatttaaaa aggcagccat tgacgcagta attgaacaga tggtctccac
      901 cggttttgaa tttgacgtag aacttctctg gcgaatccgg aattgcgggt ttcgaatcag
      961 ggagcacccc atcacatgga atgacatggg cgactcgcgg gtgaggggaa atgacgtggt
     1021 gcgaatgctc gcaagccttc tgcggctgcg tttttcatag taagatcaca tgcccctaca
     1081 gactgccgac gagagaaaac gacttgcatt ccagcgcttt gagcaggggg acttcccagg
     1141 ttcgtacgcc ctctgccgcg accttgtcgc cgggtctgcg gaccctgcgg tggccatact
     1201 ctgtgccacc aatctcttcc aaatgggacg tctcgaggag gccgaggcat atttccggga
     1261 tctcgccaat tccatcccgg agtcttccca cgttcacagc tacctcgggc ggatactcga
     1321 gaaaaaaggc gatgaccagg ccctgtccga gtttgctcat gctgtggcgc tcgaccctgg
     1381 aaacctcgag gcgttgagaa gttatgcgtc ctttctcctt gcaacaggag accccgggag
     1441 ggccgtgccg gtgctgcggc agctcgcggc gcggtcccgc cgtcccgacg actccctgct
     1501 tcttgtaagg gcgcttctgc tgaacgggat gccacgcgag gcactggacg tccgcagagg
     1561 gcctttgtgc cgcggcatcc ctgatgacga cgagtatatc cagatcctgg acgcatgcgg
     1621 agagtatgcc gagacggcca ctgcctcacg tgctgcattt gagagaacag gtaatctcgg
     1681 atatgcaagg agtgctctct tggcgctcgc ccgttttaat cctggcgctg caccggaaga
     1741 ataccagaaa tttctctctg aaaaggacga tccgggaatc aggttcgatt acgtcagatt
     1801 gttgaaggag cgcggggagt ggcgaatggc cctccgcgaa ctcggcctgc tcctgaccgg
     1861 aaagagggtc gagcccatgt tcctcctcga ggaatgcgaa atcctctggg ggcttgggga
     1921 gagagagagt gcctcgctgc acttcacacg tctgattgac cgcgaactcg aaggcctggc
     1981 tgacccggag ttccttgcca tgttactctc ccgataccgc gcgttcctcc agacgcacta
     2041 ccctgtcagg gaggcggagt cgctgctcct ggagaggctc gcaacacaac ctcacgtgat
     2101 ctgtctcctc tgcatggcat ccttctacga agatatcggc gaccatacgg aggcaaggtc
     2161 gtggtattac cgtgcatacc ggagcgactt tttctatggc ggacctgaat atgcaagatt
     2221 ctgcgagaag gaaggggatg tgagggagtg cgagaaagtg atgctctacg tgataaatag
     2281 catcagaaaa acgggggacc tcgtccgggt ggcggggatg gtcatggagg acacacacgc
     2341 tctctatcgt atgccccgcc tcatggaaag gctccgggaa cgtcttgagg agaaggcctc
     2401 ggggctctcc tcacgcgggc tcgaactgct cgcactcagc ctgcttgtct ctgcctctca
     2461 ctgcctcgag agaggggact acgctcgttg caagcgttcc tgccttgagg ggctcgactt
     2521 tatcccgccc ggttctcacg acattcacgc agaagatttc ctcgtgctca tcgatgaatg
     2581 caagcgccag tcgctctgcg atctcccggt cctggtatac gcccccctgc caggcgatct
     2641 gaaggatccc ggctccgaat tgccggaaat ccaccttgac cttgacgaga atgagaagaa
     2701 gatcgtggat ttcctgcggg tccaccagca ggccaccgag accgaactcc ggatgctcct
     2761 tgggactcgc agggcaggcg gaatggtgaa taggatcatc cagaaggcat cttctgcggg
     2821 catcgtggtg atagagaaga gaggggtggg aaaagatgga gagatctatg cattcaagaa
     2881 gtcctgatat tcagcctgcc gacaggctgg agagccagaa cattatcaat gccctgcgaa
     2941 ggggtaccgt tcctgcaagc ggcctggaga ggatcgcagt cggccttgac atagaggagg
     3001 atgtcgtcgc ggcgcagctt gatttcgttg cccgcggagg aggggacatc aagttcatac
     3061 ggggcgatta cgggagcggc aagacgttcc tcgtggcgag ggctcttgag atagcccgtg
     3121 aaaaagggtt cgtaaccgct cacgtggtca tatcctcttc ttctcccctg cacaagacca
     3181 agggtatcta ccagcagata tgtgcgagtc ttcgcacccg cgaggaggag cacgcgcaga
     3241 agtctatcat cgactcctgg ctcttcggga tagaggagcg gatcctcaag gtgagcgacc
     3301 gaacgctctc cgaagaggtc ctcgagaagg ccacccttgc tgagatcgaa actgcgcttg
     3361 ccgggatatc ggagttgaac acctcgctcg cggcggcgct ccgcacctac taccgggcaa
     3421 acaacgcagg ggacttccag aactcgcagg ccgcgatcgg ctggatttgc gctgaaccga
     3481 acgtcggacg tgaattcaag cagaaagccg ggatacgcgg ggacgtggac gaagccacgg
     3541 cgttcacgtt cctgcgcgga ctgctccgca tcatcgtgag tgccgggtac agcgggcttt
     3601 cggtcgcaat cgacgaagtg gagaccacac agtcgctccc ccggaaccag cgcgagaaag
     3661 gataccggac cctgcagatg atcgtcgact ccctggaccg gggagacctc ccgaattcct
     3721 acttcatctt taccggaact cccgcgttct tcgatggtcc ccgcggtatc cgcacgctcc
     3781 cacctctcta tgaccgcatc agcgtgatcc agacggggga agggttcagg aacccccgcc
     3841 agccgcagat aaccctcgcg aagttcgatg caaagaaact cgagcaggtg gccctcaagg
     3901 tgatggatat ctactcggcc gcatacactg aagtggacag atcgagggtc tctcaccggt
     3961 tcatcagggc catgatcaag aaggtgacgg gaaagttcgg cgggagggtc gacgtgatcc
     4021 cgaggatatt cttaaaggag tatgtcgatg tccttgacaa gtgcgagctc tacgaggact
     4081 ttgacccctg ggagcagtac cactttgaga ccgatcacct caagggcgag ttaaaagagg
     4141 aggaggaagc ggtgatggtg gtggagttct gactcttatc acacgatgta acagcaagac
     4201 ctgaaatggt ctggttcccc ggcgaccgtt ctccccctga tcccccatca caatattgat
     4261 gccttttttt cgactcatat taggtatgag tgtgcacctc gcaccgttcc tctttgtcgc
     4321 aggctgcctc atgctgcttg caggttttgc gtcaggaggg atcactctcg atgcccagcc
     4381 acacaccccc tttattggag agcgggtgca catcagcggc atttcagata tccccaacac
     4441 cattgccgta tacctctttg tgacaggccc aggcctcaac cgggcggggg tcaccctgga
     4501 gaacctccat ctcacggccg gatcgggttt ctttacctct gcatttgtcc atccggatgg
     4561 gagatttgaa tacgagtgga atactggttt tgtggttggg cagctgatcc ccgcaaccta
     4621 caggatctat gtcgtgaacg tgccgctgaa ccttgatcgc cttaccgaca gggacgagat
     4681 ctcgctatcg cacatcgatg tgacgttctc caggccccct tctccggaaa tcgggatacc
     4741 ttgggggata cctgcccttt ccctggcatt tgccgccgca gtcctctttt cctggcggcg
     4801 taaacgcgga taatatcccc ggcgccacct gcccacccgg aaaacgtgtg agaaatgtgt
     4861 gtgcccatgg tgcccggcac accagaggcg gtcagtggac ttgcgttttc cggatcggtt
     4921 gccctcgtcg tcctggtttt tgaaaaaggg tatagtgtca ttttactact tatctcgctc
     4981 acaaatcaca atgaggaatt aaggaaaatc cagtcacgaa aatccgggag aaagaagtgc
     5041 gggttgttgg tctcacttca catatgcgat tgaccccgcc gtccccaaag ggcgccccgg
     5101 cggcgattca aaaacgtttc acattggcct tgaaaacgaa attatcatct gatattcaca
     5161 aaactacatg gcaacccgcc tcaatgtaac gcgattatat tcaccggttg aataaaaagg
     5221 tcggtggcga gattgtcact ctaccataaa aaaattacaa aaatggagga cccgccttca
     5281 atgcaactct cttcggatcc tgactgacc
//
LOCUS       DAOQYR010000126         5293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_15871_length_5293_cov_9.672394, whole genome shotgun sequence.
ACCESSION   DAOQYR010000126 DAOQYR010000000
VERSION     DAOQYR010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15871_length_5293_cov_9.672394"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1082
                     /locus_tag="PKM87_10895"
     CDS             201..1082
                     /locus_tag="PKM87_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNQ30616.1"
                     /translation="MTDIPREEYILKCTSACAGCSDSLTLRYVLKAAGPETVLVVPAC
                     CTSVIQGIYPQTAMNIPVYNVAFAAAAACASGMSAAFEAAGKKTNVIVYAGDGGTVDI
                     GIQALSGAFERGANFLYICYDNEAYGNTGMQRSGATPAGARTTTTPAGKGQSKKDLDA
                     IVAAHNPPYMATACASYPKDLFSKVQKALSIRGPKFIHVLAPCPPGWRYSSERSIEMG
                     RLAVRSGMWILYEREYGKVTINGPSKAAMIRPIPLEEYLDAQGRFKGICPEAVAMLRS
                     LAERNAKRLKREEEGIC"
     gene            1076..2188
                     /locus_tag="PKM87_10900"
     CDS             1076..2188
                     /locus_tag="PKM87_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNQ30617.1"
                     /translation="MLTVATGNKAVAAAVKDACPSVVAAYPITPQTEIVEQIAEYVTS
                     GELKSQYIPVESEHSAMAACIGASITGVRTFTATSSHGLHYMHEMVNWAAGARLPIVM
                     ANVNRALGPGWNIWAEHTDAFQERDTGWLQVYVGSVQEAYDATLMAFRIAEHESVLLP
                     VMVNLDGFLLSHSMHPLQSVEIRDFIPPIHLPHAIDTANPAGYGTLTGPDQYFRFRWD
                     IERSMRDSVNVIRETEEDFRHRFGREYHPVEEYRCDDADVVVVAMGTLGKEAEVAVDL
                     LRKEGVKAGSMRIRWFRPFPRLDLSGRDVVVIDRDYSFGFGGVIARSLAATEGAKPFS
                     VIAGLGGQEVTYDDIAGFVRDRRPGEEFWFGVMPDV"
     gene            2181..2708
                     /locus_tag="PKM87_10905"
     CDS             2181..2708
                     /locus_tag="PKM87_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNQ30618.1"
                     /translation="MYEIRIHSRGGQGGVTAARLLALAAIRDGKYATACPFYGAERRG
                     APVVSYVRIDDHPIRVYSQIRHPDLVLILDTSVMDVVDVLNGMKPGGRVLVNATDHRE
                     MKGFPSFSVDLTGIALRENLVVAGNPILNTAVLGALAKMGIVTMESAKMAIQEMFSDK
                     RNVTAAEAAYAELVA"
     gene            2705..2956
                     /locus_tag="PKM87_10910"
     CDS             2705..2956
                     /locus_tag="PKM87_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ30619.1"
                     /translation="MSTRMRLAISRPTRGACGKTGSWRVFRPIVDLEKCNSCGICAMF
                     CPDAAISEDLEIDLDFCKGCGICAHECPKKAIRMEREEK"
     gene            complement(3075..3443)
                     /locus_tag="PKM87_10915"
     CDS             complement(3075..3443)
                     /locus_tag="PKM87_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30620.1"
                     /translation="MQDRFIFTTFRTNCYHCGKDADQVIKAVPYQAQVSCSNCGATRI
                     FVPRIQDVNKPGSFTRIGCYDLWNLVAEAPCRNCNVHGPHDLAIGCSHFTVRCRNCGF
                     THFYKFDLEYIAQCPIGEES"
     gene            complement(3543..5201)
                     /gene="thsA"
                     /locus_tag="PKM87_10920"
     CDS             complement(3543..5201)
                     /gene="thsA"
                     /locus_tag="PKM87_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HNQ30621.1"
                     /translation="MSQQLGGQPILILKEGSSRTRGRDAQGMNITAAKAVASAVRTTL
                     GPKGMDKMLVDTIGDVVITNDGVTILKEMDIEHPAAKMMVEIAKTQDDEVGDGTTSAV
                     VIAGELLKKAEDLLEQDVHPTVIAHGYRQAAEKAQEILQSIAVTIKAKDSAMLKKIAE
                     TAMTGKGAEGSREKICELVVKAVTMVADEEGTVDKDYIKVEKKVGGSIDDCEIIEGVL
                     IDKERVHPGMPRKVKNAKILLLNAAVEFKKTEVDAEINITSPDQLQAFLDEEERMIKN
                     IVDKVVDSGANVLFCQKGIDDIAQHYLAKAGIFAIRRVKKSDMEKLARATGGSMVSSI
                     DAISKDELGYAGLVEERKISGEEMIFVEECKNPKAVSIMVRGGTEHVVDELERAIEDA
                     LRVVSVVFEDKKLVAGGGAPEIELSLRLREYAASVGGRAQLAIEAFASALEIIPRTLA
                     ENAGLDPIDMLVELRAAHEKGKKTYGLNVFEGKAEDMLKSGVVEPLRVKTQAVASAAE
                     AATMILRIDDVIAASKTAAPQGPPGGGGMGGMGDMGGMGGMGDF"
BASE COUNT         1007 a         1585 c         1595 g         1106 t
ORIGIN      
        1 cggcactctg aacccttcga gaagggttct ttggcctgat acgcccactt tttttcgcca
       61 tatctcccca tccagttgcc gggcaggatg atttcccctg ctcataagcg attcttatcg
      121 aaaatatggg caatttcctg tttttttcga attaaatata ccttattaca gatggcggac
      181 aacatttact gctgagtttg atgacggaca ttcccaggga agaatacatt ctgaaatgta
      241 cttcggcatg cgcggggtgc agcgactcgc tcactctccg gtacgtgctg aaggcggcag
      301 gacccgagac cgtgctggtg gtccctgcat gctgcaccag cgtgatccag ggcatctatc
      361 cccagactgc catgaatatc cccgtataca atgttgcctt cgccgcggcg gccgcctgcg
      421 cctcggggat gagcgcggca ttcgaggcgg cggggaagaa gaccaacgtg atcgtctacg
      481 ccggtgacgg gggaaccgtg gacatcggca tccaggccct ctcgggtgcg ttcgagcggg
      541 gtgcaaattt tctctacatc tgttacgaca acgaggcata tggaaataca gggatgcagc
      601 gctccggtgc aacccccgcg ggagcgagga ccaccaccac ccctgcaggg aaggggcagt
      661 ccaagaagga cctggatgcg atcgtcgccg cgcacaaccc cccatacatg gccactgcgt
      721 gtgcctccta cccaaaggac ctcttctcga aggtgcagaa ggccctctcc atccgcgggc
      781 cgaagttcat ccatgtcctc gctccctgcc caccggggtg gcgctactcc tctgaaagaa
      841 gcatcgagat gggcaggctc gcggtgagat ctgggatgtg gatcctctat gagcgcgagt
      901 acgggaaggt caccatcaac ggaccctcga aggcggcaat gatcaggccc ataccgctcg
      961 aggagtacct ggacgcacag gggaggttca agggcatctg ccccgaagca gtggccatgc
     1021 tgagatcgct cgccgagcgg aatgcaaagc gcctgaagag ggaggaggaa gggatatgct
     1081 gacggtcgca acagggaaca aggcggtggc cgcagcggtg aaagatgcct gcccgtccgt
     1141 tgtcgcagcg taccccatca ccccgcagac agagatagtg gagcagatcg cggaatatgt
     1201 cacttcgggg gagttaaaga gccagtacat cccggtggag agcgagcact cggccatggc
     1261 ggcgtgcata ggcgcgagta tcacaggagt gcggactttc accgcgacga gttcgcacgg
     1321 tctccactac atgcacgaga tggtaaactg ggccgcggga gcccggctcc ccatcgtcat
     1381 ggcaaacgtg aaccgcgccc tcgggccggg atggaacatc tgggccgagc acaccgatgc
     1441 gttccaggag agggacacgg ggtggctcca ggtatacgtg ggatccgtcc aggaggcata
     1501 cgacgcgacg ctgatggcat ttcgaatcgc agagcacgag tcggtcctcc tccctgtgat
     1561 ggtgaacctg gacggtttcc tcctctccca cagcatgcac ccgctccaga gcgttgagat
     1621 cagggacttc atccccccca tccatctccc gcatgcgatc gacacggcaa acccagccgg
     1681 ttatggaacc ctgacaggac ctgaccagta cttcaggttc cggtgggaca tcgagcgatc
     1741 aatgcgcgac tctgtcaacg tcatcaggga gaccgaggag gacttcagac acagattcgg
     1801 cagggagtac caccccgtcg aggagtatcg ctgtgacgat gcagatgtgg tggtggtcgc
     1861 gatggggacc ctcggcaagg aggccgaggt tgccgtcgac ctcctccgga aggagggggt
     1921 gaaggccggg agcatgcgta tccgctggtt ccgcccgttc ccaaggctcg acctctcggg
     1981 gagagacgtc gtggtcatcg accgtgacta ttcattcggc ttcggagggg tgatcgcccg
     2041 gagccttgct gccaccgagg gtgcaaagcc cttctcggtc atcgccggcc ttggaggcca
     2101 ggaggtcacc tacgacgata tcgcggggtt cgtacgcgac aggagacctg gggaagagtt
     2161 ctggttcggg gtgatgcctg atgtatgaga tccgcattca ctccaggggc gggcagggag
     2221 gggtcaccgc agcacggctc ctcgcccttg ccgccatccg tgacgggaag tatgccaccg
     2281 cctgcccttt ttatggcgcc gagcgccggg gagccccggt ggtctcctac gtgcgcatcg
     2341 acgaccaccc catacgcgta tacagccaga tccggcaccc tgacctggtc ctgatccttg
     2401 acacgagcgt catggacgtc gtggacgtgc tgaacgggat gaagccggga gggcgcgtcc
     2461 tggtgaatgc aaccgatcac cgcgagatga agggattccc ttcgttctca gtcgacctca
     2521 ccgggatcgc tctccgtgag aacctcgtgg tggcgggaaa cccgatcctg aataccgcgg
     2581 tgctgggagc ccttgcaaag atgggtatcg ttaccatgga gtcggcgaag atggcaatcc
     2641 aggagatgtt ctctgataaa aggaatgtaa cggctgccga ggcggcgtac gcggagctgg
     2701 tcgcatgagc acgaggatgc gcctggcgat cagcaggccc acccggggtg catgcggaaa
     2761 gaccggttca tggcgagtct tcaggccgat cgttgacctg gagaagtgta actcctgcgg
     2821 gatctgcgcg atgttctgcc ccgatgcggc aatctcggaa gacctcgaga tcgacctcga
     2881 cttctgcaag ggatgcggga tctgcgcgca tgagtgcccg aagaaggcca tccggatgga
     2941 gagggaagag aaataggagg tcttccccgc tcccccgaac ccacccttat tttttcatct
     3001 tttcagccgc tcaatcgcgt ggccctgccg aatgccggtc cctcactctc cttaaggggt
     3061 gctaccggct gcgctcacga ctcctctcca atcgggcact gcgcgatata ctcgaggtcg
     3121 aacttgtaga agtgcgtgaa cccacagttc ctgcaccgga ccgtgaagtg gctgcacccg
     3181 atggccaggt catgagggcc atggacattg cagttcctgc aaggagcctc cgcgaccagg
     3241 ttccagaggt cataacagcc gatccttgtg aagcttcccg gtttgttcac gtcctggatc
     3301 ctgggaacga agatccgggt tgcgccacag ttgctgcagg agacctgggc ctggtaggga
     3361 acggccttga tgacctggtc cgcgtccttc ccgcagtgat aacagttcgt cctgaacgtg
     3421 gtgaaaataa accggtcttg catgtatcag tgtctgcact gccggataaa agattcccct
     3481 ttcacaggag agagaaagta accaggggta tgtatggaaa aaaaggttgg ccggcagggg
     3541 gatcagaagt cgcccattcc gcccattccg cccatgtcgc ccatgccacc cattccgccg
     3601 ccacccggcg ggccctgcgg ggctgcggtc ttggacgcgg caatcacgtc gtcgatcctg
     3661 aggatcatcg ttgccgcctc tgcggcgctt gcaaccgcct gggtcttgac gcggaggggt
     3721 tcgacgactc cggacttcag catgtcctcg gccttgccct cgaagacgtt gaggccgtat
     3781 gtcttcttgc ccttctcgtg tgcggcacgg agctcgacca gcatgtcgat ggggtcgagt
     3841 cccgcgttct ccgcaagggt gcgggggatg atctcgagtg cgctcgcaaa ggcctcgatg
     3901 gcgagctgtg cacgaccgcc cacgctcgcc gcatactcgc ggaggcggag cgagagctcg
     3961 atctcgggtg caccgccgcc ggcgaccagc ttcttgtcct cgaagaccac cgagaccaca
     4021 cggagcgcgt cctcgatggc gcgctcgagc tcgtcgacca cgtgctcggt gccacccctg
     4081 accatgatgg agactgcttt cgggttcttg cactcctcga caaagatcat ctcctcaccg
     4141 gagatcttcc tctcctcgac gaggcctgca tacccgagct catccttgct gatggcatca
     4201 atgctcgaga ccatgcttcc tccggtagcg cgtgcaagct tctccatgtc actcttcttg
     4261 acccggcgga ttgcaaagat tccggccttg gcaagatagt gctgggcgat gtcgtcgatg
     4321 cccttctggc agaagaggac gtttgcgccg gagtcaacaa ccttgtcgac gatgttcttg
     4381 atcatccgct cctcttcgtc gaggaacgcc tggagctggt cggggctggt gatgttgatc
     4441 tctgcatcca cctcggtctt cttgaactcg accgcggcgt tcaggaggag gatcttcgcg
     4501 ttcttcacct tgcggggcat cccggggtgg acacgctcct tgtcgataag gacgccctcg
     4561 attatctcgc agtcgtcgat ggacccgccc accttcttct cgaccttgat gtagtccttg
     4621 tcaacggttc cttcctcgtc ggcgaccatg gtgaccgcct tcacgacgag ttcgcagatc
     4681 ttctccctgg aaccctctgc acccttcccg gtcatggcgg tctcggcgat cttcttcagc
     4741 atggcggagt cctttgcctt gatggtcacc gcgatgcttt ggagaatctc ctgggccttt
     4801 tctgcggcct gccggtatcc atgagcgatc acggtcgggt ggacgtcctg ctcgaggagg
     4861 tcctcggcct tcttcaggag ctcgcctgcg atgaccaccg cagaggtggt cccgtcgccg
     4921 acctcgtcgt cctgggtctt ggcgatctcc accatcatct ttgcggcggg gtgctcgatg
     4981 tccatctctt ttaagatggt gacaccgtcg tttgtgatga ccacgtcacc gatggtgtcc
     5041 acgagcatct tgtccatgcc cttcggcccg agcgtggtcc gtactgcgct tgcaacggcc
     5101 tttgctgcgg tgatgttcat gccctgtgcg tcacgtccac gggtgcgtga acttccttct
     5161 ttcaggatga gaatcggttg tcctccaagt tgctgcgaca ttgctattct ccacgtgtag
     5221 cgataaaatt ggttattggt tctatataaa aattatgatt tactgacgag ctggatcagc
     5281 tcttccccgt ctt
//
LOCUS       DAOQYR010000127         5205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_16212_length_5205_cov_9.858835, whole genome shotgun sequence.
ACCESSION   DAOQYR010000127 DAOQYR010000000
VERSION     DAOQYR010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16212_length_5205_cov_9.858835"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..78)
                     /locus_tag="PKM87_10925"
     tRNA            complement(7..78)
                     /locus_tag="PKM87_10925"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44..46),aa:Ala,seq:cgc)
     gene            complement(208..1218)
                     /locus_tag="PKM87_10930"
     CDS             complement(208..1218)
                     /locus_tag="PKM87_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription initiation factor IIB"
                     /protein_id="HNQ30622.1"
                     /translation="MQEVEKLKILQTEREALRNRLKERVKEHEKKAEDHTETVCPECG
                     SRQLVHDYERAELVCQNCGLVIDDDFIDRGPEWRAFDHDQRMKRSRVGAPMTFTIHDK
                     GLSTMIDWRNRDSYGRAISSKNRAQLYRLRKWQRRIRVSNATERNLAFALSELDRMAS
                     ALGLPRNVRETAAVVYRDAVDKNLIRGRSIEGVAAAALYAACRQCSVPRTLDEIAEVS
                     RVSRKEIGRTYRFISRELGLKLLPTSPIDYVPRFCSGLNLKGEVQSRAVEILRQAGER
                     ELTSGRGPTGVAAAAIYISSILGGERRTQREVAEVAGVTEVTIRNRYKELAEKLDIEI
                     IL"
     gene            complement(1236..1460)
                     /locus_tag="PKM87_10935"
     CDS             complement(1236..1460)
                     /locus_tag="PKM87_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gar1/Naf1 family protein"
                     /protein_id="HNQ30623.1"
                     /translation="MKTIGLAVHAVGARILVVQCDAAQLPKLYSEVVDRRMKPVGRLV
                     DIFGNVSRPMAAVLCKGPCAVPAGERLFIR"
     gene            complement(1566..2216)
                     /locus_tag="PKM87_10940"
     CDS             complement(1566..2216)
                     /locus_tag="PKM87_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HNQ30624.1"
                     /translation="MYDLHTHTTMTDGEMLPIELVRRMAVLGYKTVAITDHADSTNLC
                     ELLASLQRLIPAAREYGVTLLPGVELTHVPPSQIPGLAREAKEHGARIVVVHGETAME
                     PVAPGTNSAACGCDDVDVLAHPGLITPEDALRASGHQVALEITSRGGHNRTNGHVVRV
                     ALAAGCQMVIDSDAHGPGDLLDARAKNLVGRGAGLDQGQCSRVVSLNIQEWLLRRK"
     gene            complement(2222..2494)
                     /locus_tag="PKM87_10945"
     CDS             complement(2222..2494)
                     /locus_tag="PKM87_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle subunit SRP19/SEC65
                     family protein"
                     /protein_id="HNQ30625.1"
                     /translation="MKGERILYPCYFNSALKRAEGRRVPRTIAVKDPSLLDLERALKR
                     SGLAFRTEQKQYPSRWWQKEGRVHVKWSESKEKLLKKVAGRLAPGK"
     gene            2551..3198
                     /gene="hypB"
                     /locus_tag="PKM87_10950"
     CDS             2551..3198
                     /gene="hypB"
                     /locus_tag="PKM87_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HNQ30626.1"
                     /translation="MHHVDVRLEKDVYDVNRRIAESNSRHLKAHGVRAFDLLGAIGSG
                     KTALIERLVPILKARGIKSGAIAGDVYGDDDFRRIVALGVPAVNANTGKECHLDAHLV
                     EHSLDDLDLDHIDLLFIENVGNMVCPTDFPLGAEKRVVIVSATEGDDVVNKHPMMFRG
                     CDIAIINKVDLAPLVGANLDRMENDISRYHPGMKVFRTNLKTGEGIEKFLDALLE"
     gene            3301..3678
                     /locus_tag="PKM87_10955"
     CDS             3301..3678
                     /locus_tag="PKM87_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8e"
                     /protein_id="HNQ30627.1"
                     /translation="MLWQGRSVRLPTGGRYRLTLVKRRTEIGSAPTDSHIGKERRSIV
                     RTTGGSRKVRTMRAEYANVADPKSGSVRKVKIETVDANVANPNYVRRNLLTKGAVIRT
                     ELGKARILSRPGQEGVINAVLIE"
     gene            4156..4620
                     /locus_tag="PKM87_10960"
     CDS             4156..4620
                     /locus_tag="PKM87_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2240 family protein"
                     /protein_id="HNQ30628.1"
                     /translation="MSLTITIAAPFRHARKDQLKRNEIVYFLSFKQHWMGIEQANMLI
                     ARAEEEGLIELHGDMITPLFDVSGVEIPLGYKPGSSVFERSDPVDDLLARISQQAGIA
                     RGQLVAELNRLVQERFGGNLRPEAAAVLMAKRYSVEFRDLLPALREHLVKNE"
     gene            complement(4621..5025)
                     /locus_tag="PKM87_10965"
     CDS             complement(4621..5025)
                     /locus_tag="PKM87_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6e"
                     /protein_id="HNQ30629.1"
                     /translation="MAEFKIVLSDQKTGRAYKVDVSGGAAGALIGKHIGDEVDAGSVG
                     LSGYRMEITGGSDRNGTPARRNLPVAGRKKILLSGGAGFKPVMEGQRRRKTIRGNEIT
                     ADFVQINARVTAYGDKALEEYFAKPAAEEKKE"
BASE COUNT         1120 a         1516 c         1406 g         1163 t
ORIGIN      
        1 atttcatgga cttggcggga ttcgaacccg cggcctttac gttgcgaacg taacgatcta
       61 cccctgatct acaagcccga ttggagtagt aatgttttct gcggctggat tatagttctt
      121 gctgatcagc gcctaaggcg tcaagagagt gccgggtaaa agaccagtca ccgatctacg
      181 attcaaccgg ggtgaaaaaa agatgcgtca gagaatgatc tcgatgtcga gcttctctgc
      241 gagttccttg tagcggttcc ggatagtcac ctcggtgacg cctgccactt cagctacctc
      301 gcgctgggtt ctccgctcgc cgcccaggat cgaggagatg tagatagcag ctgccgcgac
      361 accggtaggc ccgcgcccgc tggtcagctc ccgctcccct gcctgcctga ggatctcgac
      421 tgccctgctc tggacctcgc ccttgagatt caagcccgag cagaaccggg gcacgtagtc
      481 aatcggcgag gtcgggagga gcttgaggcc cagttccctc gagatgaacc ggtaggtcct
      541 tccaatctcc ttccgcgaga cacgggacac ctcggcaatc tcgtcaagcg tcctcgggac
      601 gctgcactgg cggcaggccg cataaagtgc tgcggctgca acaccctcga tgctccgccc
      661 gcggatcagg ttcttgtcga ccgcgtcgcg gtacaccact gccgcagtct ccctgacgtt
      721 cctcggcagc ccgagggccg aggccatcct gtcaagctcg gagagcgcga acgcaaggtt
      781 cctctcggta gcgttgctta cccggatgcg gcgctgccac ttccgaaggc ggtagagctg
      841 cgcccggttc ttgctggaga tcgccctccc gtaggagtcg cggttcctcc agtcgatcat
      901 cgtggagagc cccttgtcgt ggatggtgaa ggtcattgga gcccccaccc gggaacgctt
      961 catccgctgg tcatggtcaa acgcacgcca ctcagggccg cggtcgatga aatcgtcgtc
     1021 gatgaccagc ccgcagttct ggcagaccag ttccgcccgc tcgtagtcgt gcaccagctg
     1081 gcggctgccg cactccgggc agaccgtctc agtgtggtcc tctgccttct tctcgtgctc
     1141 ttttacacgc tctttgagcc tgttccgtaa ggcttccctc tccgtctgga gaattttcag
     1201 cttctcgact tcctgcacac caaatcacac cttgtctatc tgatgaataa cctctcccca
     1261 gccggcaccg cacacggtcc cttgcagaga actgctgcca tcggtctgga cacattgccg
     1321 aatatgtcca ccaacctgcc aaccggcttc attcgcctgt ccacgacctc gctgtagagc
     1381 ttcgggagct gtgccgcatc gcattgcacc acaaggatgc gcgcccctac tgcatgaacc
     1441 gccagaccaa tggttttcac acgaaaagcc tcttgttctt ctgcctgtga ggacagactg
     1501 aaggatttag taatctatat atatagaaaa taatagtata aaaacttatt gtaaaggtat
     1561 aaatatcatt ttctccgtaa aagccattcc tggatattta aagacaccac gcggctgcat
     1621 tgcccctgat cgagtccggc accccttccc accagatttt ttgcgcgcgc gtcaagaagg
     1681 tcccctggcc catgcgcgtc agagtcgatc accatctgac accccgcggc gagcgctact
     1741 ctcactacgt ggccattggt cctgttatgc cccccgcgtg aggtgatctc gagcgctacc
     1801 tggtgcccgc tcgcacgcaa tgcatcctcg ggagtgatga gtccagggtg cgcaaggacg
     1861 tccacgtcgt cgcacccgca tgccgcacta ttcgtcccgg gggcaacagg ttccatcgcg
     1921 gtctccccgt ggacgaccac gatccgggca ccgtgctcct ttgcctccct ggcaagtccc
     1981 gggatctgcg atggggggac atgcgtcagc tcgacacccg ggaggagtgt cacgccgtat
     2041 tccctggctg cgggaatcaa cctctggagg gaggccagca actcgcacag gttcgtcgag
     2101 tctgcatgat cggtgattgc caccgtcttg taaccaagca ccgccatcct gcggacgagc
     2161 tcgatgggca gcatctcccc atcggtcatg gtggtatggg tatggaggtc gtacactggt
     2221 atcacttccc tggcgcgagc cggccggcaa cttttttcag gagcttctcc ttgctctcgc
     2281 tccacttgac gtgaactctc ccctctttct gccaccaccg tgaaggatac tgcttctgct
     2341 cggtgcggaa tgcaaggccg gaccgcttca atgccctttc aaggtcaagg agcgaaggat
     2401 ccttcactgc aattgtccgg gggaccctcc ggccttccgc acgtttcagc gcagagttga
     2461 aatagcatgg gtagagtatg cgctcacctt tcatcctcat tatggtatga tggtgaacct
     2521 ataaactctg cagcatccta cacatttggt atgcaccatg tcgacgtccg gctggagaaa
     2581 gatgtctacg atgtcaaccg gcgcatcgcg gagtccaatt cccgccatct caaggcccac
     2641 ggggtgcgtg cctttgacct cctgggtgcg atcggttccg ggaagactgc actcatcgag
     2701 cggcttgtcc ccattctcaa ggcacgaggt ataaaatccg gcgccattgc aggcgacgtg
     2761 tacggggatg atgatttcag gaggattgtt gccctcggcg tcccggcggt caatgccaat
     2821 acgggcaaag agtgccacct cgatgcgcac cttgtcgagc actcgctgga tgaccttgac
     2881 cttgaccata tcgatctcct cttcatcgag aacgtgggga acatggtatg ccccaccgat
     2941 ttcccgctcg gagcagagaa acgggttgtg atcgtaagtg caacggaagg ggatgatgtg
     3001 gtaaacaagc acccgatgat gttccgcggg tgcgacatcg ccatcatcaa caaggtggac
     3061 cttgcaccgc tggtgggcgc aaacctcgac cggatggaga acgacatttc ccgttaccac
     3121 ccgggaatga aggtattcag gaccaacctg aagaccggag agggaattga aaagttcctt
     3181 gatgctctcc tggaataacc agtgaaatta aatacagggg aatttaaata ttaaagaact
     3241 ccggcaggaa ttgctcctgc tgaatcgaga gtatccgcaa caataatccg gggaaatgat
     3301 atgctctggc aaggaagatc tgtaaggctg cccaccgggg gccggtaccg tctcacgctc
     3361 gtaaagagga ggaccgagat cgggtccgca ccgactgaca gccatatcgg gaaggagagg
     3421 agatccatcg tacgcaccac gggtgggagc aggaaagtcc gcactatgag ggcggaatac
     3481 gcaaatgttg cagaccccaa gtccggcagc gtacgcaagg tgaagataga gacagttgat
     3541 gcaaacgttg caaatcccaa ctatgtccgg cggaacctcc tgacaaaggg tgcggtcatc
     3601 aggaccgaac tcggaaaagc gcgcatccta agccgccccg gccaggaagg cgtcatcaat
     3661 gcggtcttaa ttgaataatt cccgtatctt ttttggagga gtcttaaaaa atctccaggg
     3721 gtcgtctccc gtgttttgtc actgaaaatt aaggtggcgg tttcaaaagt cgtatccttg
     3781 gaatgaaccc aggtgtagga ggaggacagc tgtggatacg acttttgtac tccctattgt
     3841 attcataaaa atatatagat tatgatgtat gcatatgcca ggaaatgcct tgtgaatttt
     3901 tttcaacaat cagctgcgtt ccagatacac aaataaaact ggcttgtaac ggagtcagaa
     3961 tcccgcaggc gagttgtgtg ccagcttcat gtccatgggg agcggcaggg gaatttttcc
     4021 ccttgggacc atgaatgccc agagagattt ttcttctatt gggaacgtta caagaggatg
     4081 tgtggacctg ccctgcgagc ggtatgggag cattgatggc ctatcgatgg agactcttat
     4141 cagggaaagg gagacatgag cctcaccatc accatcgcgg caccgttccg ccatgctcgc
     4201 aaggaccagc tgaagagaaa cgagatagtg tatttcctct ctttcaagca gcactggatg
     4261 ggcatcgaac aggccaatat gctcattgcg agggctgagg aagaggggct catcgagctg
     4321 catggagaca tgatcacccc gctcttcgat gtgtcaggcg ttgagatccc ccttggatac
     4381 aaaccaggtt catccgtttt tgaacgttct gaccccgtcg atgaccttct tgcgcggata
     4441 tcccagcagg cgggcatcgc ccggggccag ctcgtcgcag aattgaacag gctggtccag
     4501 gaacggttcg ggggaaacct tcggcctgaa gcagcagccg tgctgatggc aaagcgctac
     4561 agtgttgaat tcagggacct gctccctgcc ctgagggagc acctggtaaa aaacgagtga
     4621 ttattccttc ttctcttcag cggcaggctt tgcaaagtat tcttccagtg ccttgtcacc
     4681 ataggcggtg acacgcgcat ttatctggac aaaatctgcc gtaatctcgt tcccccggat
     4741 cgtcttccgg cggcgctgcc cttccatgac cggtttgaag cctgctccgc ccgagaggag
     4801 gattttcttc ctccctgcca cgggcaggtt cctgcgcgcg ggcgtcccgt tccggtcgga
     4861 acctcccgtg atctccatcc ggtacccgga caggcccact gaacctgcgt ctacctcatc
     4921 accgatatgt ttgccaatca gggctccagc cgccccaccg ctcacatcga ctttgtatgc
     4981 cctgcccgtc ttctggtccg acaggacgat cttgaactct gccatttcct gatctctcca
     5041 tcatatgaat atccggttcg gttcaccggc atgctgcagg gcaccggttc tctcctcccc
     5101 acctactcaa tcgggggaag gattattccg aaggccggtg ccaagcctcc atgaagtacc
     5161 tatgaaattg gatgaaagat atattaaaga ctactttccc cagaa
//
LOCUS       DAOQYR010000128         5205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_16213_length_5205_cov_7.749126, whole genome shotgun sequence.
ACCESSION   DAOQYR010000128 DAOQYR010000000
VERSION     DAOQYR010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16213_length_5205_cov_7.749126"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            388..1278
                     /locus_tag="PKM87_10970"
     CDS             388..1278
                     /locus_tag="PKM87_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ30630.1"
                     /translation="MHKTLVSILAIAATIWFVLSSGCMTGTQNGLASQSCPDYVNPCQ
                     GMTPVPSVSINGTPIQYADVNGVRLAYRVFGSGEPILMLNGFGATMDHFYNESFLGIL
                     ATHNQVYIYDHRGMGYSSDNDAPPTMSLYSDDAAALISALGYESMNVYGASMGSSIGQ
                     QLVIDHPLRVRKLILESATYSVRIPETRLLFELIQATALNASEPAGARREAEANLVWN
                     GTWDDLSGINKSVMLLVGTDDILTPQSAAVQIAGQIDGSWLVRFKGEPHVGSRFAPVD
                     YGEITLDFLCRNESPPYATV"
     gene            complement(1935..3269)
                     /locus_tag="PKM87_10975"
     CDS             complement(1935..3269)
                     /locus_tag="PKM87_10975"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HNQ30631.1"
                     /translation="MRELCTVITVIRKVSATIILVLLVLSVVSVPVMAAGGNGGSSGS
                     DGAGSGGSGGNGNSGSSGGGAGSEAGTSTGQTGPQAQPLQEEQERKQQETVEQPGVQI
                     NQQDRDQDQGRTGVDTAVEPTNTDATRQRDRDTFLQQVSQLEQDLSRDQDREQAQVNV
                     ALYALSNTGNLTGSAGPELTRLAAEINNSYGDALQAEQQIKTRSSFMRMLVGGDRDAA
                     GLLIQYADQNQQRVQQMEQLLMNCSDCDPQVRVMLENQVQVLSTEQNRLAILGQQEQN
                     DRGLFGWLFGSSGVLAGTGQGTALTDDEKYWLNYMREEEKLARDVYLSLGSRWNLPIF
                     SNIAQSEQVHMDSVKTLLDRYGLPDPAAGKAQGEFTDPVLQKLHDDLIAQGSVSQVEA
                     LKAGVLIEETDINDLNTAIAATTHNDIKTVYENLLQGSMNHLNAFESNLARY"
     gene            3763..3921
                     /locus_tag="PKM87_10980"
     CDS             3763..3921
                     /locus_tag="PKM87_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30632.1"
                     /translation="MSEVILSSLHLVVGKVSSLRGAGWTRQCEVSERERAWPSSGTQD
                     NARSCPTM"
     gene            4230..5009
                     /locus_tag="PKM87_10985"
     CDS             4230..5009
                     /locus_tag="PKM87_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNQ30633.1"
                     /translation="MRSDKPAITANDVRIVTEDSEKQKKYESALSSETPQKSSPNARM
                     CPFCGHQLKPGAIKCINCGRYIEQFKEMELKEQGASRQSQKNSPEKCSVVNRRRFNIL
                     LIYLSILSLVLIAVSFNYLSSDYGECTTLRKTPYYSYVVTTANSDIVNAEGVIIKHGG
                     FNQLFCHKYTPLDRVGQFLYLALGIGLIGLLIHWYRKSKPITRDILIVYSIAAILFLY
                     YAFILQRPTYGSVILLLLLSPLIFAHKIMGVDYSSSDTTET"
BASE COUNT         1265 a         1268 c         1300 g         1372 t
ORIGIN      
        1 atcgccctac ctccccggcc agcccctgtt gtcaatcggg atcagcgatg ccgcgatcaa
       61 tcaggggatg gagtgggcgt ttgtggcgat gatcctggtg gtcatcggca tgttccttgc
      121 aggcctgttc atccggaaga cagggaagat agtgtgagga ggtgaaggga gaggacgaga
      181 aaaactcagg gaacgtcttt ttccagtatt gacctggtct gactgaataa aaaaaatgaa
      241 gcatgggtga aagatacgtt ttggatgtag atatcaggcg atccgtccgg gttatacgtt
      301 gcaatgggtc agctaacatc aaagcttgtt aagggtatga agcttcacta ttatctgtgt
      361 atcaacttca gtgaggtagc tctttccatg cacaagactc ttgtttccat tctggcgatc
      421 gccgccacca tctggttcgt cctttcgtcc ggatgcatga ccggcactca aaatggcctg
      481 gccagccaga gttgcccgga ctatgtaaat ccctgccagg gaatgactcc ggtcccgagc
      541 gtatcgatca acgggacgcc gatccagtac gcggatgtga acggggtcag gctggcatac
      601 cgcgtgttcg ggtccggtga gcctatcctt atgctcaatg gtttcggggc cacgatggac
      661 cacttctata atgagtcgtt cctcggcatc cttgctaccc ataaccaggt atacatctat
      721 gaccaccggg ggatgggcta cagcagcgac aacgatgcac ccccgactat gtcactctac
      781 tctgacgatg ccgccgccct gatatccgct ctcgggtacg agagcatgaa tgtgtacggg
      841 gcttccatgg ggtcctcgat cggccagcag ctggtcatcg accacccgtt acgcgttcgt
      901 aagttaatac tggaatccgc tacctacagc gtgcgtattc ccgagaccag acttctcttc
      961 gaacttatcc aggcgacggc attgaatgct tcggagccgg ccggtgcccg aagagaggcg
     1021 gaagccaacc tggtatggaa cggcacctgg gatgaccttt caggtatcaa caagagcgtc
     1081 atgcttcttg tcggtaccga tgatatcctg actccccagt cggcggcggt ccagattgcc
     1141 ggtcaaatcg atggttcctg gcttgtccgg ttcaagggcg aaccgcacgt cgggtcccgg
     1201 ttcgcaccgg tcgattacgg ggagatcaca ctcgacttcc tctgcaggaa cgaatcgccg
     1261 ccgtatgcaa cggtgtaaat cttttttttt ttttttgagg gtactccatc catttctgct
     1321 gaaaaacagg atgtggtatc accctgaaaa ggtgattttt ctggtatgtt caaatcccat
     1381 accccggtca gggaagggct acaccaggag tgattattct tcaatgaggc gatttctcgt
     1441 tggtattctc cattttatcc tgcagcggtc attgataagg gttgaatggt cgctgcagta
     1501 ccaactggta tatctggtgg gttaataggc ttttcactat aaaattcctc taaaaaaact
     1561 gaatgtcgcg aatccaacct gttcagggtg catcacatcc gagcaacagg ccacagtgaa
     1621 gagaaaggat agatcaggat tcattctccc tgagggggga gattcagcgg tcaggcgctc
     1681 ttcgttggga taatccggaa cgcatggaag gatatccatg attatgcagg attcgctctt
     1741 gcagcactca tcatcctgca ccttctgctc cactggaaat tcttaaaggc cgttccgtca
     1801 tgcttcagga agaaggcatg cgggtttggt catgagtgtt tcctttcttt ttgattgaat
     1861 tctccaattt tttttcaatg atgggagcaa aatgggtaga taccatacca acaaaaaaga
     1921 atatacattc ttttttagta tctcgccagg tttgattcga atgcattgag atggttcatg
     1981 gacccctgca gcaggttttc atacacagtc ttgatgtcat tatgcgtggt tgctgcaatt
     2041 gccgtgttca ggtcgttgat atcggtttcc tcaatcagga cccctgcctt gagtgcttcg
     2101 acctgggaca cgctcccttg agcaataagg tcatcatgga gtttctggag aacaggatct
     2161 gtgaactctc cctgagcctt gcctgccgcc gggtccggta agccgtatcg gtcaaggagg
     2221 gtctttacag aatccatgtg gacttgctcg ctctgggcga tgttcgaaaa tatcgggagg
     2281 ttccagcggc tgccgagcga gagatagaca tcccgggcga gtttctcttc ctccctcatg
     2341 tagttcagcc agtatttttc atcgtccgtc aatgcggtcc cctgaccagt accggccagc
     2401 acccccgaac tgccaaacaa ccagccaaac aaccccctgt cattttgctc ctgctgtccc
     2461 aaaatagcga ggcggttctg ttctgtcgac agcacctgca cctggttttc cagcatcacc
     2521 cggacctgcg ggtcgcaatc ggaacaattc atgagcaact gctccatctg ctggacgcgc
     2581 tgctggttct gatcagcata ctggatcaac aacccggctg cgtcccggtc accgcccaca
     2641 agcatccgca tgaaagaact ccgggtcttt atctgctgct ctgcctgaag ggcatctcca
     2701 tacgaattgt tgatctctgc tgccaggcga gtcagttccg gaccggcact tcccgtcaga
     2761 tttccggtat tggagagggc atagagtgcg acattgacct gtgcctgttc ccggtcctga
     2821 tcgcgggaga ggtcctgttc cagctggctg acctgctgga gaaacgtatc acgatctctc
     2881 tgccgggtgg catcggtgtt ggtgggttca accgcggtat cgactccagt cctgccctgg
     2941 tcctggtcgc ggtcctgctg gttgatctgg acccctggct gctcgaccgt ctcctgttgt
     3001 ttacgctcct gctcttcctg gagaggctgc gcctgtggac ctgtctggcc tgtcgaagtc
     3061 cctgcctcac ttccggctcc accacccgaa ctgccgctat tcccgtttcc tccggatcct
     3121 ccagagccgg ctccatctga tcccgatgac cctccgtttc ccccagcagc cattactgga
     3181 acactcacta ctgagagaac gagaaggaca agtatgatcg tggctgatac tttacgaatc
     3241 actgtgatca ccgtgcacaa ttcgcgcaag ctaccagtgt agggagagaa tacaaaaata
     3301 tgatccttcc ttcgattgga aggatgttcc cacgggtctt ttgcaggaaa aatacagaaa
     3361 ccggcggcaa tactggcaat ttttgggccc ttatcggcat tttgcgccgg attccaacga
     3421 ttttctttta ataaccctat caaaaagatt aaactgagtg gggaatttta aggatcaagc
     3481 ccgctcctgg gcggccaggt cggtgcatcc ggcgggctgg ggataccggg gcctgggccg
     3541 tcgtctgatc ctggtgctgg ttgctctcct atcacacccc aatgattttc atcaccagac
     3601 gccgtgaccg ctccagtaaa cgaagttact gcaagcatcg caagtaatag aataatgatc
     3661 acatgtttca ttggttcact cccttctgcc ggtcttcccg atgattatca agggtaaccg
     3721 gctctgaata ggcacttctt tttctttgat aaataattat tgatgtcgga agttatactc
     3781 tcgagtcttc atcttgtggt cgggaaggtg agcagtctgc gaggagcggg atggacaagg
     3841 caatgcgagg tgagcgaacg cgagcgagca tggccgtcat caggaaccca ggacaacgcg
     3901 agaagctgcc ccacgatgtg agcggaggtg cccgggagca ccagacgatg aggaggagcg
     3961 gccctgtgac cgagcgaaca aggtggggcc tgtcatattc aatacaattt ttgtctaata
     4021 attggcaatt gaggatattc tgtccttatt ctgataagaa ttgtcgattt tataaagaaa
     4081 aattaaaatg gctaaaagta ttggtaattt actattgatt ggcgcattag ggaggaattc
     4141 ttatcaaaga agatgaggtt gattttgatc taatcagaaa gaagtatgaa aatggtgaaa
     4201 taacccttca gaaataccag gaatatcttt tgaggtctga taaaccagca atcactgcga
     4261 atgacgttag aattgtcaca gaagactccg aaaagcaaaa aaaatatgaa tctgcgttat
     4321 catctgaaac accccaaaaa agctcaccaa atgcccgaat gtgtcctttc tgtggtcatc
     4381 aattaaaacc tggggcaata aagtgcatta attgtggtcg ttatattgaa caattcaagg
     4441 agatggagct caaagagcag ggtgccagca gacaatccca aaaaaattct cctgaaaagt
     4501 gttcagtcgt aaatagaaga agatttaata ttcttttgat ttatttgtcg attttatcgt
     4561 tagtattaat agcagtatca tttaattatc tcagttctga ttacggggag tgtacaacac
     4621 ttcgaaaaac accttattat tcgtatgtag taacgacagc taattctgac attgtaaacg
     4681 cagaaggagt gattattaaa cacggagggt ttaatcagtt attttgccat aaatataccc
     4741 cgctcgatag agttgggcaa tttttatatc tcgcgcttgg aattggccta ataggtcttt
     4801 taattcattg gtaccgtaaa tctaaaccca taacgcgcga catactgatc gtatactcta
     4861 tcgcagccat tctctttctc tattacgcat ttattttaca aagaccgacg tatggttcgg
     4921 ttattcttct actgttgtta tctccattga tttttgctca taagatcatg ggggttgatt
     4981 attcttcatc agatactact gaaacatgaa aaatgggaag tattcacaaa aaatttcgtt
     5041 tttacgaaga gcagtcctgt gaagcaatct gcaaggattg ggagcagaat gtgacgacga
     5101 ggaatgctgt gacggagccg tgtccatcgt catatccccg aataccgctg ggagcggacc
     5161 ggcgaccgga gcgaacatgg tggtggcgtt ataacattca attca
//
LOCUS       DAOQYR010000129         5202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_16221_length_5202_cov_9.089955, whole genome shotgun sequence.
ACCESSION   DAOQYR010000129 DAOQYR010000000
VERSION     DAOQYR010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16221_length_5202_cov_9.089955"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PKM87_10990"
     CDS             complement(<1..411)
                     /locus_tag="PKM87_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30634.1"
                     /translation="MVAGLLSLTVLPAGAGDTVTVDVSRIPLVFIENQGQKSAEVLYH
                     ASAAGHSIFFTPDSVVCAWGGDDTRPVSSVAITIAGQNPDTCVKGEDLLPGTANFLIG
                     DDPEKWVTSVPTFGKVRYEGVLPGVDIVYYGTQGA"
     gene            994..1494
                     /locus_tag="PKM87_10995"
     CDS             994..1494
                     /locus_tag="PKM87_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HNQ30635.1"
                     /translation="MLWENALIGIVQLIIAIILAVVALYIGFSVLTRITRGIDEEKEL
                     ARGNAAVGIIIASVFFAIALVVQSGISGISVGISKALSVGLLSADGITAIVVAFIQLI
                     LGILLAVGAIYLALSILDKLTKGVNEFEEIRKGNVAVALEMAGVIVATAVIIQSGVIG
                     ITTALL"
     gene            complement(1652..3166)
                     /gene="lysS"
                     /locus_tag="PKM87_11000"
     CDS             complement(1652..3166)
                     /gene="lysS"
                     /locus_tag="PKM87_11000"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNQ30636.1"
                     /translation="MEIRQSDLAFDQQRIEKIQELRNSGVPLYPPEFGGREPVASIKG
                     RHEGIGHEKGEEQVCTAGRLYTVRNHGKTIFADLQDESGRIQLYLRKNDIGEEKFELF
                     LRTIERGDIVGVRGHIFRTKVGEVTLWVDEITLLAKALCSLPEKFHGLKDIEKRYRQR
                     YVDLIVNQDVKETFRTRSRIISLIRSYLTDRGFLEFETPILQPIYGGANARPFTTFHH
                     YLQQNLFMRIAPELYLKRLVVGGFEKVFELARNFRNEDIDTHHNPEFSMVEVYEGYRD
                     YRDMMALSEEMISSIAYQIHGTYLVPYGDTTLDFTPPWRKMTMDEAVKDLAGIDIWSH
                     SLDDLRRIAAEKKIEGWEKAENNRELLVLLFEELVEDQLVQPTFVYDFPIENSPLAKR
                     HRSREGFTERFELFICGMEVANGFSELNDPLDQLARFERQDEKRRAGDLEAQMLDYDF
                     INALGYGMPPTGGVGFGIDRFVMLLTNKNSIKEVILFPQMKPEAGADKKDISES"
     gene            complement(3389..3709)
                     /locus_tag="PKM87_11005"
     CDS             complement(3389..3709)
                     /locus_tag="PKM87_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNQ30637.1"
                     /translation="MKEEESVSKEERRAHFLEMLTEEDPSRRWKAIEALAREGDRSAV
                     DSIIRALSDDDWRVRQKAAWALGMIGDPRAVVPLRRALREESEGVKEMILEAISEITR
                     KSLD"
     gene            3872..4168
                     /locus_tag="PKM87_11010"
     CDS             3872..4168
                     /locus_tag="PKM87_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30638.1"
                     /translation="MIIHTGGEVGKQKKKKISKHLGQEYGHTPLSEDECAFLERERPR
                     VVFIGTGQHGALPLTEGARRILAGFPTVIGPTPEIIREMEQERRPFAAILHVTC"
     gene            4295..4858
                     /locus_tag="PKM87_11015"
     CDS             4295..4858
                     /locus_tag="PKM87_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNQ30639.1"
                     /translation="MRVVLLSGSPRPEGNTIQVVEECASVIREQGLEAEVISLAGMKI
                     ESCTACNRCGKEGNCVLEDGLADIIDRIREAEGFIVASPVYFGTARGDVMAALQRIGM
                     VSRSTDHFLSWKVGGPIAVARRGGHTATIQEMLMFFFINNMIVPGSTYWNMVFGREPG
                     EVWKDVEGMSTVRLFAENVARLIKKIS"
     gene            4898..>5202
                     /locus_tag="PKM87_11020"
     CDS             4898..>5202
                     /locus_tag="PKM87_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ30640.1"
                     /translation="MGGQIIEVAGLKHRYGDFEAVAGISFTVEEGEIFSFLGPNGAGK
                     STAINVLTTLLPLQEGEARVAGFDVAKTPARVRESIGIVFQEVTLDRDMTCWEILEF"
BASE COUNT         1048 a         1501 c         1474 g         1179 t
ORIGIN      
        1 tgccccctgt gttccgtaat agacaatgtc tactccgggc aggacacctt cgtaacgcac
       61 cttaccgaag gttggcaccg atgtgaccca cttctccggg tcgtcaccga tgaggaagtt
      121 cgcggtcccg ggaagcaggt cctctccctt cacgcatgtg tcgggattct gccctgcaat
      181 ggtgattgcc actgaagaaa ccggccgggt gtcatcgccc ccccatgcac acaccaccga
      241 atcaggggtg aaaaagatgc tgtgccctgc agcactggca tggtaaagga cctctgcact
      301 tttctgcccc tggttctcaa tgaacaccag gggaatcctg gagacatcca ccgtgaccgt
      361 gtcaccggca cctgccggca acaccgtgag tgacaggaga cctgccacca taataagaag
      421 tagtataaat cctgtcttca atctcatgag ttcccactcc cacctttcac cggctgcctg
      481 ctgaacatgg gggagccata gaaccctgcc tcgaggatgc cacgtagtac tgactcagta
      541 caccatgatt caggatctca tgccccggtg agaaacctta agttctgatc agattcttat
      601 taaagaatcc ctctatttga atggtgataa atgtttccct gaaattctca tttttttcgg
      661 ccttttcacc aggagcgctc gcatctttcc gaatcatgtt ttcctttcta catcatccct
      721 ttgtcatgta tcctgcccat tggaatcgat cagagagccc aatgcaggca ttctggtatc
      781 cctcgttgca tgtgagatcg gtgacagtgc tgctttcgaa atgcgagtgc cccgaagaat
      841 cagcatccat gtacgccgaa gaaagatatt tcctgtattt cgtactcccc tgcaaataac
      901 gtagaatata tggcttgttt tagaaattaa cccgaaatct ttatcaatca actatgccca
      961 tctcattaga attcacatat gaggtgaatg tcaatgttat gggaaaatgc gttaatcgga
     1021 attgtccagc tgatcattgc catcattctg gcagtggtcg ctctctatat cgggttctcc
     1081 gtgctcacac ggataacccg gggaatcgat gaagagaaag agctggcacg gggcaacgcc
     1141 gcagtgggga tcatcatcgc atccgtcttc ttcgcgatcg cgctggtggt ccagtccggt
     1201 atctcgggga tatcggtcgg gatctccaag gcgctctcgg tcgggctcct ctcggctgac
     1261 gggataaccg ccatcgtagt tgcattcatc cagctgattc tcggcatcct cctcgccgtc
     1321 ggtgccatct acctcgcact ctcaattctc gacaagctca ccaagggtgt gaacgagttc
     1381 gaggagatca ggaaagggaa cgtggccgta gccctggaga tggccggcgt catcgttgcc
     1441 accgctgtaa tcatccagtc cggcgtgatt ggcatcacga cagccctcct ttgaataatt
     1501 tttctttttc gacgttccgg cattcccccc atttcatggt gcagcagggc ctctgcctgg
     1561 ctcgcgatca tgaaaaattc actggtttcc catggcaatg ggagcgagat caaaaaaatg
     1621 agtgtttatc cccgatccag gtttgtatcc ttcaggattc agatatgtcc ttcttgtcag
     1681 caccggcttc gggcttcatc tgggggaaga ggatgacctc cttgatggag ttcttgtttg
     1741 tgaggagcat cacgaaccgg tcgatgccga atcccactcc gcccgtgggg ggcatcccgt
     1801 acccgagcgc attgatgaag tcgtagtcga gcatctgcgc ctcgaggtcc ccggcccgcc
     1861 tcttctcgtc ctggcgctcg aaccgggcca gctggtcgag aggatcgttc aactccgaga
     1921 agccgtttgc cacctccatg ccgcagatga agagctcgaa ccgctcggtg aacccctcgc
     1981 gggaacggtg cctctttgcg agcggcgagt tctcgatggg gaagtcgtac acgaatgtag
     2041 gctggacgag ctgatcctcg accagttcct cgaagagcag gacgagaagc tcccggttgt
     2101 tctctgcctt ctcccagccc tcgatcttct tctctgcggc gatacggcgg aggtcatcga
     2161 gcgagtggct ccagatatcg atccctgcaa gatctttcac cgcctcgtcc atggtcatct
     2221 tcctccatgg cggggtgaag tcaagggttg tgtccccgta agggacaagg taggtcccgt
     2281 ggatctggta tgcgatggag ctgatcatct cctccgagag cgccatcatg tcacggtagt
     2341 cgcgatagcc ctcatagacc tcgaccatgg agaactcggg gttgtggtgg gtgtcaatgt
     2401 cctcgttcct gaagttcctg gccagttcga agaccttctc aaagcccccc acgacgagcc
     2461 tcttcagata gagctccggg gcgatgcgca tgaagaggtt ctgctggagg taatggtgga
     2521 acgtagtaaa aggcctcgcg tttgcgcccc cgtagatcgg ctgcaggatg ggcgtttcga
     2581 attcgaggaa tccccggtct gtcaggtaac tcctgatgag cgagatgatc cggctcctcg
     2641 tacggaaggt ctctttcacg tcctggttca cgatcaggtc aacgtatcgc tgccggtacc
     2701 gtttctcgat atcttttaac ccgtgaaact tctctgggag ggagcagaga gcttttgcga
     2761 ggagcgtgat ctcatccacc cagagggtga cttcgcctac cttcgtgcgg aagatgtgtc
     2821 ccctgacacc gacgatgtcg cccctctcga tggtccggag gaacagttcg aacttctcct
     2881 caccgatgtc gttcttccga agatacagct ggattcgccc gctctcgtcc tggaggtcgg
     2941 caaagatcgt cttgccatgg ttcctgaccg tatacagcct gccggcggtg cacacctgct
     3001 cttcgccctt ctcgtgcccg attccctcgt gtcttccctt tatcgaggcg acgggttctc
     3061 ttcccccgaa ctccggggga tagaggggga ccccggagtt ccgcagctcc tggatcttct
     3121 caatcctctg ctggtcgaat gcaaggtcgc tctgtcttat ctccatggta tctcctggtc
     3181 ctcccttccg tcaaatccgg gatgtacatt cccctcgtgt gtggccggcc ctgccttgag
     3241 agtcccgatc ttgcgggccg gaggcggttc gatggatcgc ggccagttcc cttaaggaat
     3301 ggggctggaa gattataatg gttttacgtc aggagagatt gcggggggat gtacaggccc
     3361 gggggagaga ggaggaggct ctttctgttc agtccagtga tttacgcgtg atctccgaga
     3421 tcgcctcgag tatcatctcc tttacccctt cactctcttc cctcaacgcc ctcctgaggg
     3481 ggacgactgc ccgggggtcc ccgatcatac ccagcgccca cgccgccttc tggcgtaccc
     3541 tccagtcatc gtctgagagt gcccgtatga tcgagtcgac cgcggaacgg tctccctctc
     3601 gggccagggc ctcgatcgcc ttccagcggc gggaagggtc ctcttcggtg agcatctcca
     3661 ggaagtgcgc cctcctctct tctttggaaa cgctctcctc ctctttcatg tgcaggagat
     3721 tgggttatcc catcgaaaaa ggttgggacc ggcccggcgg ggccctgaac ttactctcat
     3781 atcaggggac tgacaaggaa ggagatggtg atgcgatacg aagaccgggt tcggctggat
     3841 cgagtatggc gggagactct acgagcacga tgtgatcatc cacaccggcg gagaggtcgg
     3901 gaagcagaag aagaagaaga tatcaaaaca cctcgggcag gaatacggcc acacccccct
     3961 ctcggaagac gaatgtgcat tcctcgaacg ggagcgtccc agggtagtct tcatcggcac
     4021 agggcagcac ggcgcgcttc ccctgaccga aggggcaagg cggatcctcg cggggtttcc
     4081 caccgtcatc ggccccactc ccgagatcat cagggagatg gagcaggagc gccggccgtt
     4141 cgcggccatc ctgcacgtta cctgctgaaa gggtggcatc ggtcatcgca atctctctcg
     4201 caagggtgcg cgggtgagcc gtgcatggcc cgcatcgcgg ggaccggccg tagaactaaa
     4261 taaatgcggc acccagattt ccctgaggtg aataatgaga gtagtgcttc tctccggaag
     4321 cccgcgcccc gaaggcaaca ccatccaggt cgtcgaggag tgtgccagcg tgatacgtga
     4381 acaggggctc gaggccgagg tcatctccct tgcggggatg aagatcgagt cctgcaccgc
     4441 ctgcaaccgc tgcgggaaag aaggcaactg cgtcctcgaa gacggccttg ccgatatcat
     4501 cgaccgcata cgggaagcag aggggttcat cgtggcctca ccggtctact tcgggacggc
     4561 ccggggagat gtgatggcag cgctccagcg gatcggcatg gtatcccggt ccaccgatca
     4621 ctttctctcc tggaaggtgg gaggcccgat tgccgttgcc cgcaggggcg gccataccgc
     4681 aaccatccag gagatgctga tgttcttctt catcaacaac atgatagtgc ctggatcgac
     4741 ctactggaac atggtcttcg gacgcgagcc cggcgaggtc tggaaagacg tcgaggggat
     4801 gagcaccgtg cggctgtttg ccgagaatgt ggcgcggctg atcaagaaga tctcctgaag
     4861 gggaaaggcc ccctgttatt ttccgaggtt gtgcagcgtg ggtgggcaga tcatagaagt
     4921 cgccgggctt aagcatcgct acggggactt tgaggccgtg gcaggcatca gtttcacggt
     4981 tgaggagggg gagatattct cgttcctcgg cccgaacggc gccggcaaga gcaccgccat
     5041 caacgtgctc accacgctcc tccccctcca ggagggtgaa gcacgggtgg cgggattcga
     5101 tgtcgcgaaa acacccgcga gggtccgcga gtccatcggg atcgtcttcc aggaagtgac
     5161 gctcgaccgg gacatgacct gctgggagat cctggagttc ca
//
LOCUS       DAOQYR010000130         5193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_16258_length_5193_cov_10.445504, whole genome shotgun
            sequence.
ACCESSION   DAOQYR010000130 DAOQYR010000000
VERSION     DAOQYR010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16258_length_5193_cov_10.445504"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PKM87_11025"
     CDS             <1..648
                     /locus_tag="PKM87_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNQ30641.1"
                     /translation="INLINYQDPDGVETIRGMRDIAKGGGGFFYTAARVDVDGRSLYI
                     PKIDYAEPVDHEWWVFSGIIAPEYTRAATGNLTGVQLRYYTREELFDRVNEAVAFAKE
                     NGKEKTLAEINNPEGRFVTGDLFVWASSRDGVLLADPFWKSGIGQNHWEFADPHGMKV
                     TQVGIQAMENGTGFSHALFPNTALNGTADVHKLIYMKAVDDTWWIGSGIYGLRVE"
     gene            980..1441
                     /locus_tag="PKM87_11030"
     CDS             980..1441
                     /locus_tag="PKM87_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30642.1"
                     /translation="MKIPLFLLLLVLAIVTCGCTDQAPDESPAPITGTLQAHAENASV
                     PHIIGTWSGTGIGHIQNEGFHQPAEVVFHFNEQNGYAFAGKKEYARPDGKIHSEAFSG
                     VFSPGGDIMICDSGSGYTLGSFTGPDRMELRYAEDGDEARAYFLQLVRQKT"
     gene            1698..2546
                     /locus_tag="PKM87_11035"
     CDS             1698..2546
                     /locus_tag="PKM87_11035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ30643.1"
                     /translation="MAGSLELEPTAALVMAWAKDLYREGSASLFYSRLDLSAGRQMKR
                     ECDSVCPWYPEVMMNRKWFIRHLALEAVSGACGHCQVVILAAGKSPLALELLDGHPEK
                     IAAVIEIDIAGMEEKQRIYAAVSPRAAKRIRCVQADISDREGTGGAIAATGTYDPLRT
                     TLVVFEGISYYLPPEVSSGVLSLFATEDRQNTVILDSLLPCRFVKDDRRYISRGIWGI
                     IHRDCKFQNTVTYSPDEMEAMLFSAGCGKVCHHAMDKMERLRTGKNRFFPTAEHGWIR
                     ISAARL"
     gene            complement(2763..3140)
                     /locus_tag="PKM87_11040"
     CDS             complement(2763..3140)
                     /locus_tag="PKM87_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30644.1"
                     /translation="MKEWIKDTAGLGTFLWLIGYLASLMLFFSPYVGIMGWIITSVFT
                     PVTIAITWWWFRERDLPLTYYFGVGLAWTLLAVVLDYVFIVQLFGASYYEVDVYFYYA
                     LTFLIPVGVGLYLLHIRKENVTG"
     gene            complement(3375..4451)
                     /locus_tag="PKM87_11045"
     CDS             complement(3375..4451)
                     /locus_tag="PKM87_11045"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNQ30645.1"
                     /translation="MMQFGSTEPDPWTLDAHCDTGYMREFLRGPGLPPELQRVDTDTF
                     FQVTLPRLKEGNVRCLFLNTGDIGLLTSSAIIDSVHLWAGEESGKVSICHDAKEVHHT
                     VSSGKLAVVLACESSFLFQGRLDLLRNWYRLGVRVANITHGEGQEGMGWFGETALSKD
                     TLVRAASTLALQGTPSRDEFMAPESRDRLRYKEKGLTDFGRRAIDEMMRLGIVCDLSH
                     ANDATFWDALLIREESGRGSFCCTHSNCAAICPHTRNLTDEMMEALADAGGVMGLCFF
                     GEFIDEHAPALGRYVDHLLHALDVMGQDHVGIGSDYDGVPPDAFMAIPHPGRMAELWA
                     GLRDAGVPGSTLQKIAHENFLRLL"
     gene            complement(4542..4745)
                     /locus_tag="PKM87_11050"
     CDS             complement(4542..4745)
                     /locus_tag="PKM87_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFYB/HAP3 family transcription factor subunit"
                     /protein_id="HNQ30646.1"
                     /translation="MADLPIAAVVRIAKQSGAERVGSDGAAALVAKAEDYIAGLVKEA
                     NKFALHAGRKTIKEEDIALAAKS"
     gene            complement(4977..>5193)
                     /locus_tag="PKM87_11055"
     CDS             complement(4977..>5193)
                     /locus_tag="PKM87_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30647.1"
                     /translation="MGTKIGKEKIKREAGYLYYLGKDGFVWAAPMKNNKTGKKKKVGT
                     EKIAKEKGYFYYLGKDGFVGKAKMKNA"
BASE COUNT         1116 a         1511 c         1419 g         1147 t
ORIGIN      
        1 atcaacctga tcaactacca ggaccctgac ggcgtagaga ccatccgcgg gatgcgggat
       61 atcgcgaagg gcggcggcgg cttcttttac acggccgcac gggtggacgt agacggcagg
      121 agcttgtata tcccgaagat cgactatgcc gagcccgtcg accacgagtg gtgggtcttc
      181 tctgggatca tcgctccgga gtacaccagg gccgccacag gcaacctgac aggcgtccag
      241 ctccgttact acacgcggga agaactcttt gaccgcgtga acgaggcggt cgccttcgcg
      301 aaggagaacg gaaaggagaa gacccttgcg gagatcaaca atcccgaggg caggtttgtc
      361 accggcgacc tcttcgtgtg ggcctcttca agagacggcg tcctcctcgc cgacccgttc
      421 tggaagtcag gcatcgggca gaaccattgg gagtttgccg acccgcacgg tatgaaggtg
      481 acgcaggtcg gcatccaggc tatggagaac ggtaccgggt tctcacacgc gctctttccg
      541 aacaccgcat tgaacgggac tgcggacgtg cacaagctca tttacatgaa ggccgtggac
      601 gacacctggt ggatcggaag cggcatctac ggcctcaggg tggagtgagg gactcccttc
      661 actcatggga aacccctggc ttttttccac atttttttga tcggtccggg agcaggacat
      721 ttcccctgac ccgggcatcc ggcgatcccg gagatctcaa tagtggtcgg tccctgcact
      781 ctaagcaccg cggatcaatt cgctgggcaa aaggtcctgc acggtgggga agtgtgggcg
      841 cgcctcttgg cctgcacctc gatcccccgc ccgctcaacg ggtacaccag gtatgcgaac
      901 gctatcctgt aagaaggaat tccggcccgc tcaactcgcg atgaatcttt atttccttcc
      961 cggcaccagt attgccagta tgaaaattcc attgttcctc ctcctcttag ttcttgcgat
     1021 agttacctgc gggtgcaccg accaggcacc ggatgaatcg cccgccccca ttacggggac
     1081 gctccaggca catgccgaaa atgcctcggt cccccacatc atcggcacat ggtccggcac
     1141 aggtatcggc catatccaga acgaggggtt ccatcaacca gcagaggtgg tatttcactt
     1201 caatgagcag aatggctatg cctttgccgg gaagaaggag tatgcgcggc cggatggaaa
     1261 gatccattct gaggccttct ccggggtctt ttcccccgga ggggatatta tgatctgtga
     1321 cagcggttct ggctatacgc tcggatcatt caccgggccg gatcgcatgg aattgaggta
     1381 tgccgaagat ggggacgagg cgcgagccta tttcttgcaa ctcgtcaggc agaagacctg
     1441 accttcccca cctttttact atgaaaatgg ctgcgccatt ttcatgaatg cgtatgcgtg
     1501 cgtctcgaag agaatcatgt agttttttta gattcgctat cagccggatg ggccagttcc
     1561 ccgcaccctt ctccggatag tccccttcca gactcctgca ctcccacttg tcccggtctt
     1621 catgtcaggg ctcttatcct ccggaatcca actctccaac atccctaaat agccggatgc
     1681 ctgatctctc cagagagatg gcagggtccc tggaacttga gcccaccgca gcgctcgtga
     1741 tggcctgggc aaaggatctg taccgtgagg ggagcgcatc gctcttctat tcccgcctcg
     1801 acctctcggc aggaaggcag atgaaacgtg agtgtgattc cgtctgcccc tggtatcccg
     1861 aggtgatgat gaacaggaag tggttcatcc ggcatcttgc gttggaggcc gtatccgggg
     1921 catgcgggca ctgccaggtc gtcatcctcg cggctggcaa atccccgctc gcccttgaac
     1981 tcctcgatgg ccaccctgag aagatcgcag cggttatcga gatcgatata gccgggatgg
     2041 aggaaaaaca gcggatctac gcggccgtct cccctcgggc tgcgaagagg atccggtgcg
     2101 tgcaggcaga catctccgat cgtgagggga cagggggagc cattgctgcc acggggacat
     2161 acgatcccct ccgcacaact ctcgtcgtgt tcgagggcat ctcgtactat ctcccgccgg
     2221 aggtctcttc aggcgtcctc tccctttttg caacggagga tcggcagaac acggtcatcc
     2281 tggactccct cctcccgtgc cggttcgtaa aggatgaccg ccggtacatc tcccgtggca
     2341 tctggggcat catccacagg gactgcaagt tccagaacac ggtcacctac agcccggacg
     2401 agatggaggc gatgctcttt tcagcagggt gcgggaaggt ctgccaccat gcaatggaca
     2461 agatggagcg cctccggacc gggaagaacc ggttctttcc caccgcggaa cacgggtgga
     2521 tccgaatatc ggctgcacgg ctgtaagagg tgtgccctgg gggtggagat gggcgcagga
     2581 tattcacttc caatcgctat ctcccaggat ttgacgagag cgggtcgtcg tataccaaaa
     2641 ccgcatggta tgttctggac ccggtcgggc ggggcatcag gatcggcaac atccggtata
     2701 tttcgtgccg gaactaagag agagaaaaac cctctcctga aacgtccatt caggatactg
     2761 tgctaccctg taacgttctc cttgcggata tgcaggagat agaggccgac tcccaccgga
     2821 atcagaaagg tcagtgcata gtagaagtag acgtccacct cgtaatacga ggccccgaag
     2881 agctggacga tgaatacata gtcgagcacg actgcgagca gggtccatgc gagcccgacg
     2941 ccgaaataat acgtgagagg aagatccctc tccctgaacc accaccaggt gattgcgatc
     3001 gtaacaggag tgaacacgga ggtgatgatc catcccatga tgcccacgta tggggagaag
     3061 aatagcatca gggatgcaag gtatccaatc agccagagga atgtgccaag acctgcggta
     3121 tcttttatcc actccttcat acctgatctg ttcgcatcct cccagttatt gcttccgtgg
     3181 acggtcttca tgaaccggta tttgcgcggt ttggaatcgg ctcgtcccaa gagatggctt
     3241 tactggctgc catggcagag ccgggccctt actgttcacc gcgcctcctg cccctctgtt
     3301 ggtatggagc cggaggcgat ttacgatcca ttgcaggaat gttctcccct gcgtttcgtg
     3361 accggaacaa tgcgtcatag caggcgcagg aagttttcgt gagcaatttt ttggagtgtt
     3421 gacccgggaa ccccggcatc ccggagtccc gcccagagtt cggccatccg accgggatgg
     3481 ggtatggcca tgaatgcatc gggcggcacc ccgtcatagt cgcttccgat tcccacatgg
     3541 tcctgcccca tgacatcgag ggcgtgcagc aggtggtcga cataccgccc gagtgctgga
     3601 gcatgctcgt caatgaactc cccaaagaaa cacaggccca tcacccctcc tgcatcagcc
     3661 agcgcctcca tcatttcatc ggtgaggttc cgggtatggg ggcagatggc ggcacagttg
     3721 ctgtgcgtgc agcagaacga ccctctgcca ctctcttctc ttattaaaag ggcatcccag
     3781 aatgtggcgt catttgcatg ggagaggtcg cagactatcc caagccgcat catctcatct
     3841 attgcccgcc ttccaaaatc ggtcaatccc ttctccttat accggagacg gtctcttgac
     3901 tccggggcca tgaactcgtc ccggctcggg gtgccctgca gggcgagggt acttgcagcc
     3961 cggacaagag tgtcctttga gagggctgtt tcgccaaacc atcccatccc ctcctgtccc
     4021 tctccgtggg tgatatttgc cacccgcacc cccaggcggt accagttccg aaggagatcc
     4081 aggcgcccct ggaagagaaa cgaactctca catgccagca cgaccgcgag tttgccggaa
     4141 ctgaccgtgt ggtgcacctc tttcgcatca tggcagatcg aaaccttgcc actctcttct
     4201 cctgcccaca ggtgcacgct gtctatgatc gcggagctgg tgagcagccc gatatccccc
     4261 gtattcagga acaggcacct gacattgcct tcctttagcc gcggaagtgt gacctggaag
     4321 aaggtatccg tatccaccct ctggagttcg ggcgggaggc ccggaccccg gagaaattcc
     4381 cgcatatacc ctgtgtcgca atgtgcgtcg agtgtccagg gatccggttc agtgctcccg
     4441 aattgcatca tgagcgggtg tacaggcgcg atgtgaaagg gtttgacagg atcagaaaaa
     4501 gacaacagga gagacgaaaa gggttgtggg ggagaggatg attatgactt tgccgcgagg
     4561 gcgatatcct cttccttgat agtctttcgt cccgcatgca gtgcgaactt gtttgcctct
     4621 ttgacgagtc ctgcaatgta gtcttctgcc tttgccacga gcgcagccgc accgtcactg
     4681 ccgacgcgct ctgctccgct ctgttttgca atccttacaa ctgctgcaat aggtaaatct
     4741 gccaccttga ttccctcaaa gcaataatgc tttaaatagt atttatatat tcccctcgga
     4801 ccttcaccaa aaagcatcac aaaaccaagg attgaggatt gaaaggctat aaaaaaagga
     4861 tgtccccatg gactactgca tccgctcccg caatacaatt tttgccggat gtcaggggtc
     4921 gcctgcagtc ctcccgcagg aaaaaccccg tcagaatgaa aaatgaagtg tggaattcag
     4981 gcattcttca tcttggcctt gccgacaaac ccatccttgc caagataata gaaatagcct
     5041 ttctcttttg cgatcttctc agtaccgacc ttcttcttct ttccggtctt attgttcttc
     5101 atcggtgctg cccatacaaa gccgtccttc ccaaggtaat agaggtatcc ggcttcacgc
     5161 ttgatcttct cttttccaat ttttgtaccc ata
//
LOCUS       DAOQYR010000131         5094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_16659_length_5094_cov_9.343521, whole genome shotgun sequence.
ACCESSION   DAOQYR010000131 DAOQYR010000000
VERSION     DAOQYR010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16659_length_5094_cov_9.343521"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1799
                     /locus_tag="PKM87_11060"
     CDS             <1..1799
                     /locus_tag="PKM87_11060"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ30648.1"
                     /translation="PDTGYFDIFMMTLGVEEQPIAADFIADPLKGGVPLAVNFTDLSS
                     GEPSGWHWDFGDGNTSAAQTPSHIYTTPGVYDVSLIVHTPYQRSGVRFPAYIYAGNPP
                     TPAFSFDTYEGLAPLTVNFTDRSTGSPETYLWDFGDGSTSYEKDPSHTYLVPGSYLVS
                     LTAGNEFGNSTTSVEGCIVVPSGVRNELLLEIPGITCQGSIFPRRLTLNSSLVKLNII
                     NNTSIEALPYPGSGIEKIQFISDEGFAWIDQCSLEGNLTGVNISSIPLFYSSNNDNST
                     LVYQVKIDDYPSHGTFHAEVWENATPLDYEKFKTISILEDYSGICGVAFTARFGQCNI
                     SGCPEGALVFGIDSEWIDRYGWRRPVPVESDPEGAQVYFEGQYIGLTPLILPDDLPAG
                     NHTLTIYKSGYREELRNVTLGEKRESIRVIRIAEDGSHEMLPAKYISHDDACNLDYFI
                     VESPNGFSTFGAVSTSHAGSPIQFFYLTLTKIMSGVGSGGGGGGSYGGSGSVSSAASA
                     PTPVPTEDISLEKIAEMAHVQVSGPSEVQETPSPALTATHQDEHPLPKNPSTVFPSVI
                     GYTLVRNVAIVFGVILVAAILVLRWRKGGDES"
     gene            1828..2793
                     /locus_tag="PKM87_11065"
     CDS             1828..2793
                     /locus_tag="PKM87_11065"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNQ30649.1"
                     /translation="MSLADLIRQNVPPDVHVLESRGDIRALVGLLQSREYTVRNAAAD
                     ALGRLGDPALKVLHARAARGKIRERIAILEALARIRNPASIPFLSGILTGDQTSEMRW
                     IAAIALGETGSDDAVPALRNSLQDSDKYVRFGAAQSLAQLGWRPHDAEEEVRLMVARQ
                     AWSAIPGVGAVPALVLTEYLDDPDPGIRREVVSLLGMLGDPITEKFCDRAMRDPDPHV
                     RWAASLAFPHCQVPLMYLPGAMARRPRTGKRLHVAVFLNIFFLGLGYNYLGKWWGFLL
                     YQININTVMLLSLAYKTILPYPLSFGISIFAAVHTWHIMKGMPDI"
     gene            2827..3789
                     /locus_tag="PKM87_11070"
     CDS             2827..3789
                     /locus_tag="PKM87_11070"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNQ30650.1"
                     /translation="MIPAWILDLSTPDVQAMKERRDYHGLTKALRHRDPRVQWKAARA
                     LGDLGEESLDHLIGALETTWNREARLGIIEALGLIGGPRVVSPLVAQMEDRSNEVRWE
                     AAMALGETGDLAAIPALRKALEDEDKYVRYGAALSLQKLSWQPETKRDEAFLRVGLQE
                     WDTLTGPDSYAVEALGNAIRDQDKNVRLEAVKALGALRAREAAPILYRAISDPDEEVR
                     WESVLAAPACGLPLRFLPRALARRPRARKNPIVAGFLNFMLPGMGYMYIGLWWGLLIF
                     QVDVYATLWTFAATGEFISFSVLFPVYLILALHAWYMARQMPDL"
     gene            complement(3986..4276)
                     /locus_tag="PKM87_11075"
     CDS             complement(3986..4276)
                     /locus_tag="PKM87_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30651.1"
                     /translation="MTNKMGEPDSSSQAGNAAGTEEELFDLLIPPGVPRSIISDIIQK
                     FDVELVERKQRLHFANMDGDEREILAFRGTRPVVEKVQDYMFSELQKFIERK"
     gene            complement(4287..>5094)
                     /locus_tag="PKM87_11080"
     CDS             complement(4287..>5094)
                     /locus_tag="PKM87_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methanogenesis marker 14 protein"
                     /protein_id="HNQ30652.1"
                     /translation="PVDFRNPCISIDFGTTLDGRITSDVARDEENPFAKTIGNFCGLA
                     GAIPDAIVKGTGLVDSHTGTALDVFGDRSVVSDFALKGQSDVVKRYVDRAHELIDIRL
                     VPPERRRFGRVPVYADVAKESGVALIGCDAGENGSTLPALQDLGREIYAQHSLNLLNE
                     VIDRVCARMALRLVDVAAEQGHVLPNTSIGFTGRAAISGRKPDYIIEGIREREFFENP
                     NDRLVFVDDGLARGAALMGRCMNSLGKPKTPIGGVRGGPCIMARRIKIGK"
BASE COUNT         1076 a         1451 c         1443 g         1124 t
ORIGIN      
        1 atcccgatac cggatatttc gacattttta tgatgactct gggtgtcgaa gagcaaccga
       61 tcgcagcaga ctttatcgct gaccccctca agggaggagt ccccctggcg gtcaatttca
      121 ctgacctctc gtcgggggaa ccgtcaggat ggcattggga ttttggggat ggaaacactt
      181 cggcagcaca gaccccttcg cacatctaca cgacacctgg tgtatatgac gtctccctga
      241 ttgtccatac tccataccag aggtcgggtg tccgcttccc cgcatatatc tacgcaggaa
      301 acccgccgac gccggctttt tcgttcgaca cgtatgaagg gcttgctcct ttgacggtga
      361 acttcacgga ccggtcaacc gggtccccgg agacctatct ctgggatttc ggggacggtt
      421 ccacttctta cgagaaggac ccttcacaca cctaccttgt acctggcagc tacctggttt
      481 ccctgaccgc cggcaacgag ttcggtaact ctacaacttc ggtcgagggc tgtatcgtgg
      541 tcccctcagg cgtgaggaat gaactgctgc ttgagatccc tggcatcaca tgtcaggggt
      601 ccatctttcc ccgtcggctg accctgaatt cgagccttgt caagctcaat atcatcaaca
      661 acacatccat tgaggcattg ccatatccgg gatccggaat tgagaagata cagtttattt
      721 ccgatgaagg ttttgcctgg atagaccagt gctcgctcga ggggaacctc accggtgtga
      781 atatttcaag tattccgctc ttttacagta gcaacaatga caactcgacc ctcgtatacc
      841 aggtgaagat cgatgactat ccctcgcatg ggacattcca cgcggaggta tgggagaatg
      901 cgactcccct ggactacgag aagttcaaga cgatatcgat cctcgaagac tattccggga
      961 tttgcggggt cgcattcact gcaaggttcg gccagtgcaa tatcagcggt tgcccagagg
     1021 gggctctggt attcggaatc gattcggaat ggattgacag gtacggatgg agaagaccag
     1081 taccggtgga atctgaccct gaaggtgcac aggtgtattt cgaaggccag tacattggct
     1141 tgactccgct tatcctccca gacgatctgc ctgcagggaa ccataccctg acgatctaca
     1201 agtccggtta ccgtgaggaa ctgcgcaacg tgacgcttgg cgaaaagaga gagagtatcc
     1261 gggtgatccg gattgcagag gatggatccc atgagatgct tcccgcgaaa tatatctctc
     1321 atgatgatgc ctgcaacctt gactacttca tcgtagagtc ccccaatgga ttctccacgt
     1381 ttggtgcggt gtccaccagc cacgcgggaa gcccgatcca gttcttctac ctcacgctca
     1441 ccaagatcat gtcaggcgta gggagcggcg gaggaggggg tggcagctac gggggctccg
     1501 gctccgtcag ttctgccgct tcggctccga cccctgttcc tacggaggac atctctctcg
     1561 aaaagatcgc ggaaatggcg cacgtacagg tctccggacc cagtgaagtc caggaaacac
     1621 caagcccagc tctcacggcc acgcaccagg acgaacaccc gcttcccaaa aatccttcaa
     1681 cagtctttcc atcagtgata ggctataccc tcgtcaggaa cgtggctatc gtgttcgggg
     1741 tgatcctggt cgcagcaata cttgtcctgc ggtggagaaa aggaggagac gaatcttgat
     1801 cctgaaatgg ggatgggatg agcccagatg agcctggcgg atttgattcg ccagaatgtt
     1861 cctcccgatg ttcatgtcct tgagtcccgg ggagacatca gggctcttgt gggactcctc
     1921 cagagcaggg aatatacggt gaggaatgcg gccgccgatg cccttggcag gctcggcgac
     1981 cctgcgctca aggtgctcca tgcccgtgct gcccggggca agatcaggga acggatcgca
     2041 atccttgagg cgctggcaag gattcgtaac ccggccagta ttccgtttct ttcggggatc
     2101 ctcaccggag atcagaccag cgagatgcga tggatagcgg caatcgcgct cggcgagaca
     2161 gggagtgatg acgcggttcc ggcgctccgg aattccctgc aggacagtga caagtatgtg
     2221 cgctttgggg cagcacagtc acttgcgcag cttggttgga gaccgcacga tgccgaggag
     2281 gaggtgaggc tcatggttgc ccgccaggca tggagcgcaa ttcctggcgt cggcgctgtc
     2341 ccggccctcg tgctgaccga gtatctcgat gaccctgacc cgggcatcag gagggaggtc
     2401 gtctccctgc ttggaatgct gggagacccc atcacggaaa aattctgcga cagggcgatg
     2461 cgtgaccctg acccacacgt tcgctgggcg gcgagccttg cattccctca ctgccaggtt
     2521 cccctgatgt accttcccgg ggcaatggca aggcggccac ggaccggaaa gcgcctgcat
     2581 gtggcggtct ttctcaacat ctttttcctg gggctcggct acaattatct cggcaaatgg
     2641 tggggcttcc tgctgtacca gataaacatc aacaccgtga tgctcctctc ccttgcatac
     2701 aagacaatcc tcccttaccc cctctcgttc ggtatttcaa tttttgctgc tgtccatacc
     2761 tggcacatca tgaaggggat gcctgatata tgaacaatcg gtcatcatgc gaggagggat
     2821 tgtgaagtga tcccggcatg gatcctggat ctttcgaccc cggatgtcca ggcaatgaag
     2881 gagcggaggg attatcacgg gctcacgaag gcgctccgtc accgtgaccc gagagtgcaa
     2941 tggaaagctg cccgtgcgct gggggatctt ggtgaggaga gcctggacca cctgatcggg
     3001 gcgctggaaa ccacctggaa cagggaagca cgtcttggga taatcgaggc actcgggctg
     3061 attggaggcc ccagggtggt ttcacctctc gttgcgcaga tggaggacag gagcaacgag
     3121 gtgcgctggg aagcggcaat ggctctcgga gagacaggag acctggctgc aatcccggcg
     3181 ctgcgcaagg cacttgagga cgaggataaa tacgtccgct acggggcggc cctctccctc
     3241 cagaaactct cctggcagcc ggagacaaag agggacgaag cctttctgcg tgtgggtttg
     3301 caggagtggg atacactgac ggggcccgac tcgtatgcag tggaagcact gggaaatgcc
     3361 atcagggacc aggataaaaa tgtcagactt gaggcggtga aggcgcttgg cgcccttcgt
     3421 gcaagggagg cagcccctat cctttaccgg gccatcagcg atcctgatga ggaggtccgg
     3481 tgggaatccg tgctggcagc acctgcctgt gggctgcccc tcaggttcct cccaagggcc
     3541 ctggcacgcc gcccccgtgc aaggaagaac ccgatcgtcg ccgggttcct caacttcatg
     3601 ctccccggga tgggatacat gtacattggt ctctggtggg gcctcctgat cttccaggtg
     3661 gatgtgtatg cgaccctctg gacctttgca gccaccggcg aattcatctc attttccgtc
     3721 ctcttcccgg tttatctgat ccttgcactc catgcatggt acatggcgag gcagatgccg
     3781 gatctgtgac gctaacagga accgaaatgg cggtccctgc agacgaggcg caagagaacc
     3841 aaagggtaaa tcatgtggct acttcaccat ggagcgtaaa tcaccggagg ggatatagac
     3901 gggtcccctt cagcggcgaa tgcaatcagt ttcatcataa gatgctcccg gatccgggtg
     3961 aaaagaatag tgcaggagag gagtgttatt tcctttcgat gaatttttgc agttcactga
     4021 acatgtagtc ctggactttc tccaccaccg gacgtgtccc ccggaatgcg aggatctccc
     4081 gctcgtctcc atccatgttc gcgaaatgaa gacgctgttt cctttcaacc agctccacat
     4141 cgaatttctg aatgatgtcc gagatgatcg agcgcggtac tccggggggt atcaggaggt
     4201 cgaagagttc ctcttctgtt cctgctgcgt ttccagcctg gctggatgaa tccggttctc
     4261 ccattttatt ggtcatgaaa ttcccctcat ttgccaatct tgatcctcct tgccatgatg
     4321 cagggaccac cacggacccc tccaatgggc gtctttggct ttccaagcga gttcatgcac
     4381 cggcccataa gcgccgcacc ccttgcaagt ccgtcatcga caaagacaag tcggtcattc
     4441 gggttctcaa agaattctct ctctctgatc ccttcaatga tataatccgg ctttcgcccc
     4501 gatatcgccg ctcttccggt gaacccgatg ctggtatttg gcaatacatg cccctgttcg
     4561 gccgccacgt ccacgaggcg gagcgccatg cgtgcgcata cacgatcgat gacctcgttg
     4621 aggaggttca ggctgtgctg ggcatagatc tccctgccaa gatcctgcaa tgcagggagg
     4681 gtgcttccat tctctccggc atcgcagccg atgagggcga cccccgattc ctttgccaca
     4741 tcggcataca caggtaccct tccgaacctc cgccgttcag gaggcacgag ccttatgtca
     4801 atcaattcat gggcacggtc cacatagcgt ttcaccacgt cgctctggcc tttcagtgcg
     4861 aaatcgctca cgacactccg gtcgccgaac acgtccagcg cagttcctgt atgggagtct
     4921 acgagcccgg tccctttcac gatagcatcg ggaattgcgc cggcaagccc gcagaagttg
     4981 ccgatggtct tggcaaacgg gttctcttca tcccgggcca cgtcgctcgt gatgcgcccg
     5041 tcgagcgtgg tcccaaaatc gatcgagatg caggggttcc ggaaatcgac aggg
//
LOCUS       DAOQYR010000132         5029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_16936_length_5029_cov_7.656815, whole genome shotgun sequence.
ACCESSION   DAOQYR010000132 DAOQYR010000000
VERSION     DAOQYR010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16936_length_5029_cov_7.656815"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1546)
                     /locus_tag="PKM87_11085"
     CDS             complement(110..1546)
                     /locus_tag="PKM87_11085"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNQ30653.1"
                     /translation="MPTIPENGAMHPATPGNLGVIIILAAGALAALVLSRDLLRSGRA
                     HGVPLKRRLAAFALFAIPSIAMGVVLHWYLRYPGESGGGLNPELVVAAIPLLLYLVLS
                     SIALMAGALISWPFRGTLKVHVVAGALATVIAFMGLIAGDPSRRFVTALMLLSAVLST
                     LVARWQEGTSWGGLAGSGDRDDRSGPADVPVEPGTPSLNNKGMFPPELTEKYEVTEFV
                     GSGGLARVYRAQNLQTGEEVALKIPLQSDEKTGKSFLKEIVGWEGLNHENIVRVSGMN
                     ILPIPYVEMEYIPKTLSDLPKPLPPRSAARICRDVARGLLFAHERGIIHRDIKPQNIL
                     ITYEGVPKITDWGMSKVMGVTGMPTITGFSLAYAAPEQLAPQTYGETDQRTDIYQLGC
                     VLYELITGTVPFPGNDMVQVSAHILSETPRLPSTMNPDALPLDSIIMRCLEKSPENRY
                     QDVRDLIRDLDAYLLSAETSDRYNIFED"
     assembly_gap    1720..1819
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1820..2453)
                     /locus_tag="PKM87_11090"
     CDS             complement(<1820..2453)
                     /locus_tag="PKM87_11090"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNQ30654.1"
                     /translation="MSRSFPATPAILLTIVLLLLPVPLHAADTYIITASAGPGGKISP
                     SGDVTVAKGSDQLFVIEAFQGYRIENVMVDQRSLGAIPLFTFNKVNTNHVISATFSPV
                     FGSLQVHSRPGGAEVLIDNTFYGTTDPGGPVTFTRIAPGIHSIRMLLAGYQEYKTEAQ
                     VNEGQTTIVPTAILVPVPTTVPTTTIPTTTPTTTATTTIPTTTPTTTATTT"
     gene            complement(2484..3275)
                     /locus_tag="PKM87_11095"
     CDS             complement(2484..3275)
                     /locus_tag="PKM87_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNQ30655.1"
                     /translation="MTRESQDAARTMVLDEVPDYFNELSDYLEVLSNSQRLKILKMLE
                     KKPRDVRTIASEISTSYENTKKHLDKLMGMGLIRKEVGLGQQTSKGVHPVWKYSLVPG
                     AMEAVVRNLGIFSNMRIRMADADLKRRLDEVRHAIEGDITGHHPMLILLGGPEDGKCF
                     LVPDDGVRIGRVDPTKPGPSDCEGDVILAREYTSVSRVSKPHAVFRKEGTKWLIEDRG
                     STGGTFLNSTPLQKRVPVEVCDGDLIELAKGTSGARLLVILPGRK"
     gene            3444..4184
                     /locus_tag="PKM87_11100"
     CDS             3444..4184
                     /locus_tag="PKM87_11100"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HNQ30656.1"
                     /translation="MAFAMPFGKDKRGQARQVKSMRMEQFRIPGSIGEARAIQEEIRR
                     EVGEEPGVPSTNPSLVAGADASYGSASAYASVVLMKFPSLAFCSRAEARSGIQYPYVP
                     GYFSFREIPPLLEAFSLLGRFPDLVMVHGHGYAHPRRTGLATHLGYHLQIPSIGIAGS
                     GMKGMDAREPSLLQGSATPVWMDGEMVGTLLRTREGSAAVYVSAGYRTTLDQAREIVM
                     QCIRANRFPEPLVQADRATRRMRKRCER"
     gene            4481..4753
                     /locus_tag="PKM87_11105"
     CDS             4481..4753
                     /locus_tag="PKM87_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30657.1"
                     /translation="MTSSLGRPNILLKWPVRQLITYRLVTEAVKGGRVTEVTEKTLQK
                     KYKEKAFAAGCEGEKVVGITRFMALGEFFSLALGAMQEIRGDLRLE"
     gene            complement(4833..>5029)
                     /locus_tag="PKM87_11110"
     CDS             complement(4833..>5029)
                     /locus_tag="PKM87_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HNQ30658.1"
                     /translation="SIDRLKCVVCNCCVEVCPAHCLSMETGYSPSMTTHGGIEVVHIT
                     YVKPERPPRKEPEEKSEQGS"
BASE COUNT         1042 a         1264 c         1520 g         1103 t
ORIGIN      
        1 aaaaacggct tcgctgttta ccatggacta cacatgtgta attcatggat ggaaatcttg
       61 gggtttattt tttaaattgc ggttcctgcg atagcgaccg atacaggttt caatcttcga
      121 atatgttgta gcggtcactg gtttcagcac tgagaaggta tgcatcaagg tctctgataa
      181 ggtcccttac atcctgatag cgattttccg gactcttctc gaggcagcgc atgataatcg
      241 aatcgagcgg cagtgcatct ggattcatgg ttgacgggag tctgggtgtt tccgagagga
      301 tgtgggcact gacctgcacc atgtcattgc ccgggaacgg aacggtcccg gtgatcaatt
      361 catacaacac gcaccccaac tggtatatat cggtgcgctg gtcggtctct ccatatgtct
      421 ggggtgccag ttgttcgggt gccgcgtagg cgagagaaaa accagtgatg gtgggcattc
      481 cagtcacccc catcaccttg ctcatccccc agtccgtgat cttcggcaca ccctcatagg
      541 ttatgagaat gttctggggc ttgatatccc ggtggataat ccctctctcg tgtgcgaaga
      601 ggaggcccct ggcaacgtcg cgacagatcc gtgcggcaga tcttggcgga agaggttttg
      661 gcagatcgga gagggtcttt gggatgtact ccatctccac atacgggatg ggcaggatat
      721 tcatgccgct cactctcacg atgttctcgt gattcagtcc ctcccagccc acgatctcct
      781 tcaaaaagga cttccccgtc ttctcgtccg actgcagcgg gatcttcagg gcaacctcct
      841 cccctgtctg gagattttgt gccctgtaca ccctggcaag cccccctgac cctacaaact
      901 cggtgacctc atatttttcc gtgagttcgg gagggaacat tcccttattg ttaagggagg
      961 gtgtgcctgg ctcaaccggt acatccgcag gcccgctgcg atcatccctg tcgccgcttc
     1021 ctgccaggcc gccccaagat gttccctcct gccaccgtgc cacgagggtg gagagaaccg
     1081 cggagaggag catcagcgcg gtgacaaaac gccttgaagg gtcgcccgcg ataagtccca
     1141 tgaacgcaat gacggtcgcc agcgctcctg caaccacatg gaccttcagt gttccccgga
     1201 acggccagga aatgagtgca cctgccatga gcgcgatcga tgagagtacc aggtacagta
     1261 acaggggaat cgccgcgacg accagctcgg ggttgagacc acctccactc tcccctggat
     1321 accggagata ccagtgaagg actacaccca tcgcgatgga tggaatggca aagagtgcga
     1381 acgccgcgag gcgccttttc agggggacac catgcgccct tccggagcga agcaggtccc
     1441 gggagaggac gagagccgcc agcgctcccg ctgcaagtat gataatgacg cctaggttcc
     1501 caggagtggc cggatgcata gccccgttct cgggaatcgt tggcattgtg agatttggga
     1561 cagcggtctg ttcagtcgtc gggatagtgg tggtgggagt cgtggtgggg atagtggtag
     1621 tggcggtggt ggtgggagtc gtggtgggga tagtggtagt ggcggtggtg gtgggagtcg
     1681 tggtggggat agtggtagtg gcggtggtgg tgggagtcgn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnt agtggtagtg gcggtggtgg tgggagtcgt ggtggggata
     1861 gtggtagtgg cggtagtggt gggagtcgtg gtggggatag tggtagtcgg gacggtggtc
     1921 ggaacaggga caaggattgc ggtcgggact atcgtggtct gcccctcatt gacctgtgct
     1981 tcagtcttgt actcctggta tcctgcaagg agcatgcgta tggaatggat tccgggagca
     2041 atgcgggtga atgtcacagg cccgcctggg tcggtagtgc cgtaaaaggt attatcaata
     2101 aggacctctg ctccccctgg tcttgaatgg acctgcaatg atccaaacac gggggagaac
     2161 gtcgcggaaa tgacgtggtt cgtattgacc ttgttgaatg tgaacagagg gatcgcgccg
     2221 agggatctct ggtcaaccat gacattctca attctgtaac cctggaatgc ctcaatcacg
     2281 aagagctgat cgctgccctt ggcaaccgtc acatctcctg agggagagat cttcccgccg
     2341 ggaccggcac ttgcggtgat gatataggtg tcggcggcat gcaggggaac gggcaggagg
     2401 agaaggacga tagtaagaag gattgcgggg gtggctggga atgagcggga catggaaggc
     2461 gaaggagcag gatcgttgac aggttacttc cggcccggaa ggatgaccag aaggcgggca
     2521 cctgaggtcc ccttggcgag ctcgatcaag tccccatcgc acacctccac cgggactcgt
     2581 ttctgcaggg gtgtgctgtt caggaatgtc ccgcctgtgc tccccctgtc ctcgatcaac
     2641 cattttgtac cttctttcct gaagactgca tggggcttgc tgaccctgct gactgaggta
     2701 tactcccgtg ccagtatgac atctccctcg cagtcagaag gtcccggctt cgttgggtcc
     2761 acccggccaa ttctcacgcc atcatcaggg acaaggaagc atttcccatc ctctggaccg
     2821 ccaaggagga tcagcatggg gtggtgacct gtaatatctc cctcgatcgc atgccggacc
     2881 tcatccagtc gtctcttcaa gtctgcatcg gccatcctga ttcgcatgtt ggagaagatg
     2941 ccaagattcc gcaccaccgc ctccatggcg ccggggacaa gagagtattt ccagaccggg
     3001 tgcacccctt ttgaggtctg ctgaccaagt ccgacttctt ttcttatgag ccccattccc
     3061 atgagcttgt ccaggtgctt ctttgtgttt tcgtaactgg tagagatctc ggatgcgatg
     3121 gtccgcacat cccggggttt cttctcaagc atcttgagta tcttgaggcg ctggctattg
     3181 gagaggactt ccagataatc agagagctcg ttgaaatagt cagggacttc gtcaagtacc
     3241 atggtccgtg ctgcgtcctg cgactccctg gtcatccttt tttccacctt gtatatagtg
     3301 tgcccctgca ggaacaaaaa tattctccga aagaatgcag ttgagtcggg ttcacctggc
     3361 aggaatcacg gttgtggggg atacacgaca gcctcccgca ggtcttacca atcgggactc
     3421 catggcaaac agacgaatga gatatggcat ttgccatgcc gttcgggaag gataaaagag
     3481 gacaggcacg ccaggttaag agtatgagga tggagcagtt ccgcatccca ggatccatcg
     3541 gagaggccag ggcaatacag gaggagatcc gtcgggaagt tggggaagag ccgggtgttc
     3601 ccagcacgaa cccttcactg gtcgccgggg ctgacgcaag ctacggcagt gcgtctgcgt
     3661 atgcttccgt ggtgctgatg aaatttccct ctcttgcgtt ctgctcgcgg gccgaggccc
     3721 gatcaggcat acaatacccc tatgttccag gctatttttc gttcagggag atcccacccc
     3781 ttctggaggc attctccctt ctcggccgct ttccggatct cgttatggtg cacggccatg
     3841 ggtatgcgca cccccggaga acaggtcttg ctacgcacct tggttatcac ctgcagatac
     3901 caagcattgg gattgccggc agcggaatga aaggcatgga cgcccgcgaa ccctcgcttt
     3961 tacaggggtc agcgacacca gtatggatgg atggggagat ggtgggcacg ctcctcagga
     4021 cacgggaagg gagtgctgca gtctatgtct cggcagggta ccgcaccacc cttgaccagg
     4081 ccagggagat cgtcatgcaa tgtatccggg ccaatcggtt cccggaacca ttggtgcagg
     4141 cggatcgtgc aaccagaagg atgcgcaagc gatgcgagag ataatatgat cggatgagag
     4201 tgtctggttt gcatgcagag agaggaagcc cgttgcctga tgtgtgagtc aattaaaaaa
     4261 gagagcctcc gcgggcacag tcttgctatg gcggcgatca cgatggcact tgccacacaa
     4321 atgggatgag acctgcagtg ctgggagata attgggctcc ttcacgatat cgattacgag
     4381 cgtgtggaag gcgacatggg caggcacggc gaggaaggat accaaatgct gaataatcgg
     4441 ggtatcgacg aggatatcgc aggccctgtc cggaggcaca atgacttcct ctttggggcg
     4501 tccgaatatc ctgttgaagt ggcccgtcag gcagctgata acatatcggc tggtcaccga
     4561 agcggtgaaa ggaggtcgtg ttactgaggt gactgaaaag accctccaga aaaagtacaa
     4621 agaaaaggca tttgctgcag gatgcgaggg agagaaggtg gtggggatca cccggttcat
     4681 ggcactcggt gagttctttt cactggctct tggcgccatg caggagatac gtggcgatct
     4741 caggctcgaa tgaaaggatg caccagccga ttcacctggc cggcatgggg gaaggcaaaa
     4801 aaggaagggt aggtacgatt gcctttccgg gttcaggagc cctgctcgct cttctcttcc
     4861 ggttccttgc gggggggcct ttccggcttg acgtaggtga tatgcaccac ttcaatgccc
     4921 ccgtgggtcg tcatgctggg ggaatagcct gtttccatgc tcaaacagtg ggcggggcat
     4981 acctccacgc agcagttgca caccacgcac ttcaggcggt cgatactcc
//
LOCUS       DAOQYR010000133         4835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_17834_length_4835_cov_5.893933, whole genome shotgun sequence.
ACCESSION   DAOQYR010000133 DAOQYR010000000
VERSION     DAOQYR010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17834_length_4835_cov_5.893933"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PKM87_11115"
     CDS             <1..255
                     /locus_tag="PKM87_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30659.1"
                     /translation="PLRAIFFLEQSQENTIEPIDKKAVIRMLPFYVIKPLVTADWWEK
                     ILDLIQVISQEVPVYRLKLDRSGKVRELLDEFLCTVPGKQ"
     gene            301..1494
                     /locus_tag="PKM87_11120"
     CDS             301..1494
                     /locus_tag="PKM87_11120"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ30660.1"
                     /translation="MSTLASSDYCDTYDLSESPLFERRHPLLPSLDIELTERCNNNCV
                     HCYINLPRNDAKAISRELTTGEWKDILNQAAEIGVLTVHFTGGEPLLRDDFAELYLHA
                     RHLGMRVLLFTNGRCITPNLADLFSRIPPLEKIEVTVYGMQPTTYDVVARCPGAFDEF
                     WRGVQLLLQRKVPFIVKGALFPQNKGDRERFEAWAETIPWMEGVPSYSMFFEMRGRRD
                     SRKKNENIAQLRTTPKDGLSVLTRDEIRYRKDMAQFCQKYMGASGIRLFSCGAGLSLS
                     IDAYGIIQPCLRLRMPELCYDIRKGTLKEAYTVFFPRVREIHAQNPDYLKRCANCFIK
                     GLCDQCPAKSWSEHGTLDTPVDYYCTIAHEQARYLGLIEMGESAWEVTDGRERVERMT
                     KELIK"
     gene            complement(1515..2078)
                     /locus_tag="PKM87_11125"
     CDS             complement(1515..2078)
                     /locus_tag="PKM87_11125"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ30661.1"
                     /translation="MSNILLSRRRSLIFISAVKRFIQKTAATIVHGIQSRKGYRKLIK
                     KLNPAVVIREKYSESPGTPTPSFVWTFTAEWKSKTIGTLHLVRYPPEYAPYLGFWLMG
                     LQVEWNYRGLGTGEHLCLAAIDRARVEGAEEIHLLVNERNLPAMQLYEKIGFEQISVP
                     AFDEVLFREKKQTGTTRILMRLTFSNQ"
     gene            2191..2511
                     /locus_tag="PKM87_11130"
     CDS             2191..2511
                     /locus_tag="PKM87_11130"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HNQ30662.1"
                     /translation="MQELDMDARYIPSGNIVARVIEGELILVPLVSGIGDIDDELFSV
                     NETGKVIWDRLDGKASLAQIVQLLADQYSVSLGEMEQDVRGFVGELLKRKMIVDARAS
                     PASS"
     gene            2483..3007
                     /locus_tag="PKM87_11135"
     CDS             2483..3007
                     /locus_tag="PKM87_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HNQ30663.1"
                     /translation="MPEPPLLRHKRYDADEIALSSAALLELLMAVHEKGADFRFRATG
                     TSMYPTIRDGDIITVSPLGGIKPGVYEVVAFRHPGNGKLVVHRVLALSPDSFVAGGDN
                     SQSDGTIPCSHLLGVVTKVERNGSPLFWPDKRRYPFLATVYFVTGYWYLMVRKRGYGI
                     ILKLFGKRSGETIP"
     gene            3004..4134
                     /locus_tag="PKM87_11140"
     CDS             3004..4134
                     /locus_tag="PKM87_11140"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ30664.1"
                     /translation="MTAEFATVTPLPQYHLWDQTKGKRIPYSFDLELTARCNNSCRHC
                     YINLPRNDRAAIKKELTPEEVASIADQAVSLGSLWCLITGGEPLLREDFSDIYTMLKK
                     KGLLVGVFTNACLISDEHIQLFRTYPPRDIEVTVYGATKETYEKVTRRPGSYEAFRRG
                     LDLLLEGGVKVRLKAMALRSNVHELPEISRFCRTYTKDYFRFDPLLHLRYDGNKKRNA
                     EIMVERLSPDEVVAIEQADQERADSLLRGCNELIIPALKNEESDRLFTCGAGRGSFTV
                     GHDGYFRLCSSLWHPGSVYDLRKGSLKDAWEHFVPQVLDLRSSDRDFLEKCRKCPIIN
                     LCLWCPAHASLESGRMDAWCQYFCDVAHARAKALEKRAGLSQ"
     gene            4131..>4835
                     /locus_tag="PKM87_11145"
     CDS             4131..>4835
                     /locus_tag="PKM87_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30665.1"
                     /translation="MRSTTLPPLKISTLHQTLLRALRLVWSMAPGWTAANMLLVVIMG
                     VLPIAALYLTKVIVDSVTAGLAGTDPMVLFPQVFFYILVAAAIALSLAIARSLSELTS
                     EAQSMIVTDGVSDILHAQSIAVDLEYYEDTSYYDTLHRAQQEAPYRPTRIVNGLVQIG
                     QNGISLLGVAVLILTFSWVIALLLIAAAIPAALVRYYYARARFRFEQENTESERQAGY
                     YHWVLTDSSHAKEVRLF"
BASE COUNT         1116 a         1247 c         1276 g         1196 t
ORIGIN      
        1 cctctgcgtg ccatcttttt cctggaacaa tctcaggaga atacgatcga gccaattgac
       61 aagaaggcag tcatccggat gctcccgttt tatgtgataa aaccactcgt caccgcagac
      121 tggtgggaga agatccttga cctcatccag gttatctcac aggaggtccc ggtgtatcgt
      181 ctgaagctgg acagatcagg gaaagtgaga gagctcctgg atgaatttct ctgcacagtt
      241 cctggaaaac agtgaaattt gaatgggaca tattgctatt gttgtgaacc tcatcaggtg
      301 gtgagcaccc tggcctcctc tgactattgt gatacctacg atctgagtga aagtccactc
      361 ttcgagagac ggcaccctct tcttccctct cttgatatcg aacttaccga acggtgcaac
      421 aataactgtg tccactgtta catcaatctc ccgcgaaacg atgccaaggc catctcccgg
      481 gaactcacga ccggggaatg gaaggatatt ctgaaccagg cagctgagat cggcgtgctg
      541 accgtccact ttaccggggg ggaacccctt ctccgggatg atttcgccga actgtacctc
      601 catgcccgcc atcttggcat gcgggtgctc ctctttacca acggccggtg tatcacgccg
      661 aatctggcag acctgttctc aagaatccca cctcttgaaa agatcgaagt cacggtatac
      721 gggatgcagc ccaccaccta tgatgtggtc gcccgttgcc ctggtgcatt cgatgaattc
      781 tggagggggg tgcagctcct cctccaaagg aaggtaccct tcatcgtgaa aggggcgctc
      841 ttcccccaga acaaggggga tcgggagaga ttcgaggcat gggcagagac catcccctgg
      901 atggaaggtg tcccctccta ttcaatgttt tttgagatga gaggcaggag agattcaagg
      961 aaaaaaaatg agaacattgc ccaattgcgg accactccga aagacggact cagcgtttta
     1021 acccgtgatg agattcggta caggaaggat atggcgcaat tttgtcaaaa atatatgggg
     1081 gcatcgggaa ttcgcctttt ctcctgtggt gccggtctca gtctctcgat cgatgcctat
     1141 gggatcatcc agccgtgtct ccgtctaagg atgccggagc tctgttatga tatcaggaag
     1201 ggaacattaa aggaagctta taccgttttt ttcccccgag tacgtgaaat acatgcacaa
     1261 aatcctgatt atctcaaacg ctgtgccaac tgtttcatta aaggtctttg tgatcaatgt
     1321 cctgcaaaat cctggtcaga gcatggcacc ttggataccc ctgttgacta ctattgcacc
     1381 attgcgcatg aacaagcacg gtatctcgga ctcatcgaaa tgggtgagtc tgcatgggaa
     1441 gtgacggatg gcagagaaag ggttgaacgg atgacaaagg aactcatcaa gtagaaaaat
     1501 attcagaagc gggattactg gttactgaag gtcaggcgca tcaggattcg cgttgttccc
     1561 gtctgctttt tttccctgaa tagcacttca tcgaaggccg gaacagatat ctgttcaaat
     1621 ccaatttttt cgtaaagttg cattgccggc aggtttctct cattcaccaa gagatggatc
     1681 tcctctgctc cttcgactct cgctctgtcg attgctgcaa gacagagatg ctctccggtt
     1741 cccaatcccc gatagttcca ttcaacctgg aggcccatca gccaaaatcc taggtagggc
     1801 gcatactcag ggggataccg aaccaagtga agagtgccaa tggtcttgga tttccactca
     1861 gcggtgaaag tccatacaaa agagggggta ggagttcctg gggactcgga atatttctcc
     1921 ctgataacaa cggccgggtt taatttctta atgagttttc tatacccctt tctgctctga
     1981 ataccatgaa cgatggtggc tgctgttttt tgtatgaatc gttttacggc tgatataaaa
     2041 attaagctcc tcctcctcga caataatata ttactcattt acaatggaaa acatgatact
     2101 gattctctcc aatatattcc gctagaatca gatattcaca ccgcttatca gacttatctc
     2161 aatctcttgg ttacataagg aggaacaagg atgcaggaac ttgatatgga tgcccgttac
     2221 atcccgtcgg gtaatatcgt tgcaagggta attgagggag aattaattct cgttcccctt
     2281 gtctcgggaa ttggagacat cgatgacgag cttttctccg tgaacgagac gggaaaagtg
     2341 atctgggacc ggctcgatgg aaaggcaagt cttgcccaga ttgtccagct gcttgcggac
     2401 cagtactcgg tatcattggg ggagatggag caggatgttc gcggatttgt gggagaactt
     2461 ctcaagagaa agatgattgt cgatgcccga gcctcccctg cttcgtcata agaggtatga
     2521 tgcggacgag atcgcacttt ccagtgccgc ccttctcgaa ctcctcatgg cggtccatga
     2581 aaaaggtgct gatttccggt tcagggcaac agggacgagc atgtatccca cgatcaggga
     2641 tggtgatatc attacggtat cacctctcgg aggaatcaag ccaggtgtgt acgaggtcgt
     2701 tgcattccgc catccgggca atggtaagct ggtggtgcat cgtgttcttg ccctgtcccc
     2761 cgattcgttt gtggcggggg gtgataacag tcagtcagat ggaaccatac cctgctctca
     2821 tctccttggt gtggtgacga aggttgaacg gaacggatct cccctcttct ggccggacaa
     2881 gcgccggtat ccgttccttg caacggtgta tttcgtgacc gggtactggt acctgatggt
     2941 gaggaagaga gggtatggca taatcctgaa actcttcgga aagcgatcag gggagacgat
     3001 cccgtgacag cagaatttgc aacggtgacg ccactccctc agtatcatct ctgggaccag
     3061 accaagggaa agcggatccc ctactccttc gatcttgaac tgaccgcccg ctgcaataac
     3121 tcctgccggc attgttacat taaccttccc cggaatgaca gggctgcgat caagaaagag
     3181 ctgacccctg aagaagtagc gtctatcgct gaccaggcgg tctccctcgg atcactctgg
     3241 tgcctgatta ccggcggcga accactgctc cgggaggatt tttctgacat atataccatg
     3301 ttgaagaaaa aagggctcct tgtcggcgtt tttaccaatg catgcctgat cagcgatgaa
     3361 catatccagc tttttagaac atatcctccc cgggacatcg aggtcacggt atatggtgcc
     3421 accaaggaga cctatgagaa ggtgacccgc cggccgggtt cgtacgaggc attccggcgg
     3481 ggtcttgacc ttctcctgga aggaggggtg aaggtcaggc tgaaagccat ggcgctccgg
     3541 tcgaacgtgc atgaacttcc ggaaatttcc cggttctgcc gaacgtatac caaagattat
     3601 ttccgcttcg atccgctgct gcatctcagg tatgatggga acaaaaaacg gaatgccgag
     3661 atcatggtgg agcggttgtc gccggacgag gtggtagcca ttgaacaggc ggatcaggaa
     3721 cgggcagatt ccctgctccg tggatgcaat gaactgatta tcccagccct gaaaaacgag
     3781 gaaagtgatc gtcttttcac gtgcggggcc gggagaggaa gttttacggt cggccatgat
     3841 ggttatttcc gtctctgctc ctcgctctgg cacccgggat cagtctatga tctccggaag
     3901 ggttcgctga aggatgcctg ggaacatttt gttccccagg tactggatct ccggtcatca
     3961 gaccgggatt ttcttgaaaa atgcaggaag tgcccgatca tcaacctctg tctctggtgt
     4021 cctgcccatg cctctcttga atccgggcgg atggatgcct ggtgtcagta tttctgtgat
     4081 gttgcccatg cccgggcgaa agcattggag aagcgggcag gactgtcgca atgaggagca
     4141 cgactctccc ccccctgaag atctccacgc tccaccagac gctgctccgg gcattgcggc
     4201 tggtctggtc catggctccc ggctggaccg cagcaaacat gctcctcgtc gtcatcatgg
     4261 gagtgcttcc catcgcggct ctgtacctga ccaaggtgat cgtggattcc gtcactgctg
     4321 gtcttgctgg aactgaccca atggtactct ttccccaggt gtttttttac atcctggttg
     4381 ctgctgcgat cgccctgtcc cttgccattg cacggtcgct ctccgagctg acctcagaag
     4441 cgcagtcaat gatcgtcacc gatggagtat cggatatcct ccatgcgcag tcaattgccg
     4501 ttgacctgga atattacgag gataccagtt actatgatac cctgcaccgg gcccagcagg
     4561 aagccccgta ccgcccgacc cggattgtca acggcctggt ccagataggg cagaatggca
     4621 tatcgctgct cggggtcgct gtgctcatcc tcacgttcag ctgggtgatt gctctcctcc
     4681 ttattgctgc tgctatcccg gccgctcttg tgaggtatta ttatgcccgt gcccggttcc
     4741 gcttcgaaca ggagaatacc gagagtgaac gccaggcagg gtactatcac tgggtactga
     4801 ccgactcttc ccatgccaag gaggtccggc tcttc
//
LOCUS       DAOQYR010000134         4800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_18013_length_4800_cov_9.084510, whole genome shotgun sequence.
ACCESSION   DAOQYR010000134 DAOQYR010000000
VERSION     DAOQYR010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18013_length_4800_cov_9.084510"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="PKM87_11150"
     CDS             complement(<1..1190)
                     /locus_tag="PKM87_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNQ30666.1"
                     /translation="MMRPTLLLLLLGLLAGAIFLAGCTAPQATESPAAPHVTAPPATA
                     PGDLMFLTEDYPPLNYVENGTIKGISVDLLRGVLTRMGNTVASDRITVLSWSQAYETA
                     LSRNNTVVFATVRLPEREHLFKWAGPLGSDRKVLFSMKDRSPVITSPADLDRYRIGVV
                     RDDSAHLQLVGLGVNASRIIAYPTIRSLISAMQDGTLDLWCYGETVGRYHAGKVTGDP
                     NAFSVVYTLEYQDHYYAFNKNTPDEFVAEFQSALDSLRYEPDETGITEYQRIMYRYLG
                     VSCTPEPPISREQVVSLVDYAADVLMQDTPGTIARINAGEHPFWDHETRALYIFVYDT
                     NVTIVAEADNPQLIGVNMKGKTDVAGTPFRDQIVERALAEGTGWVDYIWMIPDRNGVY
                     YKSA"
     gene            1379..1576
                     /locus_tag="PKM87_11155"
     CDS             1379..1576
                     /locus_tag="PKM87_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30667.1"
                     /translation="MHDDPPLVCCPVCRSAMVTRINEKGLWECQYHRCRAVFLLYPRI
                     PHDQAPLIREDGSWYFVSGGP"
     gene            complement(1621..2469)
                     /locus_tag="PKM87_11160"
     CDS             complement(1621..2469)
                     /locus_tag="PKM87_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PDC sensor domain-containing protein"
                     /protein_id="HNQ30668.1"
                     /translation="MNRYRNFFLIAVVTGLLLVAGCLQEQTAQIPPTPTPLAGHAAME
                     QALGNVTGSVQSALDRLDFTISDAAGALGTTGLAGPAADAVLAKAADSHPAVMNAITY
                     DQDGTVLAAEPGEAKVLVGQDLIGYAIVQKAVTEREPLMSELLLLAEGGDGVVIAHPV
                     FSMDKEFAGVVSMAFSPDALVRPIADSTASGTPYSFMVAQPGGRVLYDPDPEEVGRET
                     FNETLYAEFPEILDLARHYAAHRTGYDTYSFHRTGSGEVVSKETFWDTVELHGTEWRV
                     LVIGER"
     gene            2740..3174
                     /locus_tag="PKM87_11165"
     CDS             2740..3174
                     /locus_tag="PKM87_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30669.1"
                     /translation="MAAGASEYPALKSITASLPMEDVKPQITADANAQLGTISYYLWD
                     RTAVTKHTYDGSKVISVPWGSMLVWEVPGKVEGTSHQRGMIFMYDMFAGTVTDSLLLK
                     PNKWATLTTNPGFYVTADTGVEIIVIAPDGDIAYEAILIDIP"
     gene            3210..3425
                     /locus_tag="PKM87_11170"
     CDS             3210..3425
                     /locus_tag="PKM87_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30670.1"
                     /translation="MGIAKVLAEWKPLCRKGGYLVVSDMTLFQMDPPVKLLDFLKACN
                     VTLRNGRGENRRDQGYRAPAPLHVQAQ"
     gene            3458..3616
                     /locus_tag="PKM87_11175"
     CDS             3458..3616
                     /locus_tag="PKM87_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30671.1"
                     /translation="MAERTAALRPAVGSSPERAAVLDSLEREGEMYRKFRKSYGYTCD
                     VMQRPARN"
     gene            3883..>4800
                     /locus_tag="PKM87_11180"
     CDS             3883..>4800
                     /locus_tag="PKM87_11180"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HNQ30672.1"
                     /translation="MRRLRLLLILLLLAVVPLSPVSGSGTWGVVAAWGAEGDGAGEFS
                     GPYGIAVDESGEMIYIVDESNHRVQVFDAHGTYRAMWGEPGYDAGGEFNHPTGIAIAP
                     SGTVYVVDNYNHRVQAFDRNGGFQHFWGSYGTGEGEFNLPTDIAIDAAGDVYVTDLNN
                     CRVQKFKPDGTFINAWGSEGSGPGEFLNAEGIAAGPDGSIYVADTRNNRTQKFDTSGT
                     FLLEWGRSGDGEGEFYYPIDIAADADGNVYVTDWGNNRVQVFDASGMFLSAWGTHGTG
                     PGQFNGPTGIAADAAGNVYVTEWAANRTQK"
BASE COUNT          962 a         1362 c         1503 g          973 t
ORIGIN      
        1 tacgccgact tgtaatagac gccgtttcgg tcggggatca tccagatgta gtccacccag
       61 ccggtcccct cggcaagcgc cctctccacg atctggtccc tgaacggggt gccggcgaca
      121 tcggtcttcc ctttcatgtt taccccgatc agctggggat tgtcggcctc cgcgacgatg
      181 gtcacgttcg tgtcgtatac aaagatatag agggcccggg tttcgtggtc ccagaacggg
      241 tgctcgcctg cgttgatacg tgcgattgtc ccgggagtgt cctgcatcag cacatccgcg
      301 gcgtagtcca cgagcgacac cacctgttca cgcgatatgg gcggctccgg ggtgcaggag
      361 accccgaggt accggtacat gatccgctgg tactcggtga tcccggtctc gtccggctcg
      421 tacctgaggc tgtcgagggc tgactggaac tcggcaacga actcgtccgg cgtgttctta
      481 ttgaaggcat agtagtggtc ctggtattcg agcgtgtaga cgacggaaaa tgcattggga
      541 tcgccggtca ccttccccgc atggtacctg ccaacggtct cgccgtagca ccagaggtcg
      601 agcgtcccgt cctgcatcgc ggagatgagg gagcggatcg tgggataggc gatgatccgg
      661 gacgcgttca cgccaagccc cacgagctgg aggtgcgcgg agtcatccct gaccaccccg
      721 atccggtagc ggtccaggtc agcaggggag gtgatcacgg gggatctgtc cttcatcgag
      781 aagagcacct tccggtcgga gccgagcggg cccgcccact tgaaaaggtg ctcgcgctcg
      841 gggagccgca cggtggcgaa caccacggtg ttgttgcggg agagcgccgt ctcgtaggcc
      901 tgcgaccacg agagcaccgt gatgcgatcg gaggcaacgg tgttacccat ccgtgtgagc
      961 accccgcgga gcaggtcaac ggatatcccc tttatcgtcc cgttctccac gtagttgagc
     1021 gggggatagt cctcggtgag gaacatcagg tctccgggtg cagtcgcagg gggtgcggtc
     1081 acgtgagggg ctgcaggtga ttcagtcgcc tggggggccg tgcatcctgc aaggaatatc
     1141 gcccctgcga gcagtccgag cagcagtaaa aggagagtcg gtctcatcat ccaccatcct
     1201 tggatgagtg gttccgccgt gcatctctta tgcattgcgg aattgtcgca tgggggacga
     1261 agggcctttg cgggaaatgg tgggagggtg gcccttccct ttgggaggga gcggtccctt
     1321 cacactttca ctccccaccc gggcgatctt gtaatcatta taacgccact ctccccgcat
     1381 gcacgatgac ccgccactcg tctgttgccc tgtctgcaga tcggcgatgg taacccggat
     1441 taacgagaag ggcctctggg agtgccagta ccaccggtgc cgggcggtgt tcctgctcta
     1501 cccgcggatc ccgcacgacc aggcaccgct catacgggag gacggaagct ggtattttgt
     1561 ctcagggggg ccgtgaaccc cttcgctgcg ctgttccgaa aaacccgggg aggatctctc
     1621 tcatctctcc ccgatcacga gcacccgcca ctctgtcccg tgaagctcca ccgtgtccca
     1681 gaaggtttcc ttcgacacaa cttcgcctga tccggtccgg tggaaggagt aggtgtcgta
     1741 gccggtgcgg tgtgccgcat agtggcgggc aagatcgagt atctccggga attctgcata
     1801 gagtgtctca ttgaaggtct cgcgtccgac ctcttcgggg tcggggtcat agaggaccct
     1861 tccgccaggc tgtgcaacca tgaaggaata cggggtgcca gacgcggtac tatccgcgat
     1921 gggccggaca agggcatccg gcgaaaatgc catgctgacc acgccggcaa actccttgtc
     1981 catggagaag accgggtgag cgatcaccac gccgtctcct ccctcggcaa gcagaagaag
     2041 ttcgctcata aggggttctc tttctgttac ggccttctgc acaatcgcat agccgatgag
     2101 atcctggccg acaaggacct tcgcctcgcc aggctctgca gcgagcacgg taccgtcctg
     2161 gtcataggtg atcgcgttca tgaccgcagg gtgagagtcc gccgcttttg cgagcaccgc
     2221 gtccgcagca gggccagcga gcccggtcgt cccgagggca cctgcggcat cagagatggt
     2281 gaagtcgagt ctgtcgaggg cagactgcac gcttccggtc acattcccaa gcgcctgctc
     2341 catggccgcg tggccggcga gcggggtcgg tgtgggagga atctgtgcag tctgttcctg
     2401 gaggcagccc gcaacgagga gaaggccggt caccacagcg atgaggaaga aattacggta
     2461 cctgttcatg aggtatcatg tagactccgt gtcgttttgt tgtttttgct taggcgtgat
     2521 tacgggcgtc taccagcgca ttggagtgca caattaccgg gacattgata tcgggcaatc
     2581 tttatattcc caattcgtca tctcactctt tgaccagcag caaagggatc acgaaatttc
     2641 ctcgtctgcc tggtctaaag gaaagggggt gtgagattga acatatggga ccattctcta
     2701 ttctggcttc ttgtcatgat cgtgatttgc tctcctgtca tggcagccgg tgcatcggag
     2761 tatccggcat taaagagcat tacagcctcc ctgccgatgg aggacgtaaa accgcagatc
     2821 acggccgatg ccaatgcgca acttggcacg atctcgtatt acctgtggga tagaacagcc
     2881 gtcacaaaac acacctatga tggttccaag gtcatttctg tgccgtgggg atcgatgctg
     2941 gtgtgggaag tgcccgggaa agtggaaggt acctcacacc agagaggtat gatattcatg
     3001 tatgacatgt ttgcggggac agtaacagat tcgcttcttc tcaaaccgaa caaatgggca
     3061 accctcacga cgaatcccgg attctatgtc accgctgata cgggtgttga gatcattgtc
     3121 attgctccgg atggcgatat cgcttatgaa gcaatattga ttgatatacc ctgaatattt
     3181 ttattggcgg gaatctggaa gcatccctca tgggaattgc aaaggttctg gccgaatgga
     3241 agcccctgtg ccggaaaggc ggataccttg tcgtttccga catgacgctc tttcagatgg
     3301 atccccccgt caagctcctg gacttcctga aggcctgtaa tgtcacactc cgcaacggaa
     3361 gaggagaaaa caggagagat cagggataca gggctcctgc acctctccac gttcaggctc
     3421 aatgaggcgg gatggacgga gcattttttc cgagccaatg gcagagagaa ccgccgcgct
     3481 gagaccggcg gtcgggtcat cgccggagcg tgcggccgtg ctcgattcgc tcgagcgcga
     3541 gggagagatg taccggaagt tccggaagtc gtacggctat acgtgcgacg tgatgcagag
     3601 accggcccgc aattaagtcc accaggacag ggtaagtgcg ctcctcgttc cagccaaaga
     3661 gttcggaccc cgcgtttcgc ttgaataccc tctcttcccc ggggaccggg agagtcggga
     3721 tggcccgtgc cgttcgggta ttgccgaaga ccagaatgat tttctggccc tggcaccgac
     3781 gagtaacagt acctggtccc ggcagcactc tccccccccc cttttttcga cccgaaaaga
     3841 cagggggcgg aacgggacca taatcaggga gttatgaaaa aaatgagacg actacgactg
     3901 ttattgatac tcctgctcct cgcagttgtg cccctctccc cggtatccgg ttccgggacc
     3961 tggggggttg ttgccgcgtg gggtgcagaa ggcgacgggg ccggggaatt ctccggtccg
     4021 tacgggatcg cggtcgacga gagcggggag atgatctaca tcgtcgacga gagcaaccac
     4081 cgggtccagg tattcgacgc acacggcacg taccgggcga tgtgggggga gcccgggtac
     4141 gatgcgggcg gtgagtttaa ccacccgacc ggcattgcga ttgcgccctc cgggaccgtg
     4201 tacgtggtgg acaactacaa ccaccgggtc caggccttcg accggaatgg agggttccag
     4261 cacttctggg gctcctacgg aacgggcgag ggggagttca accttcccac cgatatcgcg
     4321 atcgacgctg caggggatgt ctacgtgacc gacctgaaca actgccgcgt ccagaaattc
     4381 aagcccgacg ggaccttcat caacgcatgg ggctccgaag ggagcggccc gggagagttc
     4441 ctgaacgcgg agggtatcgc ggcaggccct gacgggagca tctacgtcgc agatacccgg
     4501 aacaaccgga cccagaaatt cgacacatcg gggacgtttc tcctcgagtg gggacgttcc
     4561 ggggatgggg aaggggagtt ctattacccg attgacattg ccgctgacgc ggacgggaac
     4621 gtgtacgtga ccgactgggg caacaaccgc gtccaggtgt tcgatgcctc gggcatgttc
     4681 ctctcggcgt gggggacgca tggcaccggg ccgggccagt tcaacgggcc tacggggatc
     4741 gcggctgacg ccgccggcaa tgtctacgtg accgaatggg cggccaaccg gacacagaaa
//
LOCUS       DAOQYR010000135         4689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_18576_length_4689_cov_8.437419, whole genome shotgun sequence.
ACCESSION   DAOQYR010000135 DAOQYR010000000
VERSION     DAOQYR010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18576_length_4689_cov_8.437419"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..306
                     /locus_tag="PKM87_11185"
     CDS             118..306
                     /locus_tag="PKM87_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30673.1"
                     /translation="MKKIAEYDSVIEFVAVINDEKDCLMSQDKTQDQSGCMWFNIDVP
                     KGHGIVAGDRVKVTIEKI"
     gene            complement(411..1529)
                     /locus_tag="PKM87_11190"
     CDS             complement(411..1529)
                     /locus_tag="PKM87_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNQ30674.1"
                     /translation="MAVIETLITIFLIIVIVIIFARGVVIVQPYEQGLAIRLGRYIGR
                     MNPGFRWIVPLVTNVIKMDLRTQVMDVPSQEVITKDNSPTNVDAIVYIRVIDPEKAFF
                     EVVNYRMATVALAQTSLRGIIGDMELDEILYNRDIINTKLRDILDRETDQWGVKVERV
                     EIKEVDPVGAVKQAMTEQTAAERARRAAILRADGDKRSAILKAEGTKRSMILEAEGER
                     QSKVLRSEGERISRILQSQGEAQALRILSVGAQPLDRRAITVLSLDALKEMANGQATK
                     IIFPFEVSSLMKQAARFIGASDEVPEEPAVAGVPDTSILGTIPTTEEVTEILKEMDKV
                     IRTEVDVDELKDISKGRKKTGSELVEDIEESIGREDQG"
     gene            complement(1534..1995)
                     /locus_tag="PKM87_11195"
     CDS             complement(1534..1995)
                     /locus_tag="PKM87_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNQ30675.1"
                     /translation="MVELADLSFGWILVVLGALFLVIETSSPGFFIAVPGTVMIILGV
                     LILLGVDIFSGPTGLVAGVVATVAVSMITIWLYRRINPGETTPTTISRDSLVGMEGRV
                     VRKVFPDSLSGKVLIAGQEWSARSGSGEIQPGATVRVKASEGVHIIVEEVK"
     gene            2176..2766
                     /locus_tag="PKM87_11200"
     CDS             2176..2766
                     /locus_tag="PKM87_11200"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HNQ30676.1"
                     /translation="MSTSMLEKIRGFMFNPSQAFRRVKDEEAAETVKYLMILAVFYAI
                     MSALLTAIEIFTHPFLMGLPSMGPNPVEPILFVSWIIVILVVTLVVAVIFGFWLHIWV
                     YLLGGRKGVWQTEKSVFYNLTPTLLLGWIPVIGPLVGGVWSVIIGVIGVRDLHNMPDT
                     KAAFAVVLAIVIASVLAVLVLGAMLLAVVSSIPMNV"
     assembly_gap    3261..3360
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3683..4318)
                     /locus_tag="PKM87_11205"
     CDS             complement(3683..4318)
                     /locus_tag="PKM87_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ30677.1"
                     /translation="MFTVMVIDDSPFIVDIFVTMLQRGGYQTVAAYGGQEALDTLSTV
                     KPDLILLDIMMEPMDGWETLMNIKNSPDLKNIPVMMLTAKQLTPSEAQEYGIYIEDYI
                     MKPITHKELYEAIEGQLNRRRMIENDILMATEAGVDKETIDTYRRLRKSIDINTRLLK
                     ILESTYKVSDEKMKTGDEAGLAIRSMSMNIKFQKDQLEQVRDEFFSSAKPA"
     gene            complement(4318..4677)
                     /locus_tag="PKM87_11210"
     CDS             complement(4318..4677)
                     /locus_tag="PKM87_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNQ30678.1"
                     /translation="MLKEKIKGYIERIKGIEGVLDCALISRDGIMLGNSIQGEFNEPW
                     LAAMCATLFASAESTSSILKMPPPGAVSIVIRETSIVVIGAGEKLLIIAVVADAGRQS
                     PVPRQLEAMAGEIGGSF"
BASE COUNT          932 a         1311 c         1275 g         1071 t
ORIGIN      
        1 tcctctccga gatctgccac gacctggcaa tcatcaagtg gagctattca aacagtacca
       61 cctggatggt aacgtccagg ccggtatgag gggatttttc cccatcacct ttcccttatg
      121 aagaagattg cagagtacga ttcggtcatc gagttcgtgg ccgtcatcaa cgatgagaag
      181 gactgcctca tgtcccagga caagacgcag gaccagtccg gctgcatgtg gttcaacatc
      241 gatgtgccca aggggcatgg gattgtggcc ggggaccggg tgaaagtgac catcgaaaag
      301 atctgaacgc tgccggggaa gcgcaagcgc ggaccttttc atctcttctt ttccagaata
      361 tttcccgagg agaagtgaaa agagcgggag agaatcctgg accggggcct tcacccctga
      421 tcctctctcc cgatggactc ctcgatgtcc tccaccagtt cagaccccgt cttcttccta
      481 cccttgctga tatccttgag ctcgtcaacg tcaacctcgg tcctgatgac cttgtccatc
      541 tccttgagga tttctgtaac ctcttcagtg gtgggaatgg tgccaaggat ggatgtatca
      601 ggaacaccgg ccactgctgg ctcttcaggc acctcgtcgc ttgcgccgat gaaccgcgct
      661 gcctgcttca tgaggcttga gacttcgaac gggaagatga tcttggtggc ctgcccattc
      721 gccatttctt tcagggcgtc aagggagaga accgtgatcg cccgacgatc aagcggctgg
      781 gcgccgacag agaggatgcg gagtgcctgg gcttctccct gggactggag gatacgcgat
      841 atccgctccc cttcagaccg gagcaccttg ctctggcgct caccttccgc ctccaggatc
      901 atgctccgct tggtaccttc agctttaagg atggcagatc gcttgtcccc gtcagccctg
      961 aggatggctg ctcggcgggc acgctctgcg gcggtctgct cggtcatggc ctgcttgacc
     1021 gcaccgacag ggtccacttc cttgatctcg acccgctcta ccttgacacc ccactggtcg
     1081 gtctcgcggt cgaggatgtc cctgagtttg gtattaatga tgtcccggtt atagaggatt
     1141 tcatcgagct ccatgtcacc gatgatccct ctcaggctgg tctgggcaag agctacggtt
     1201 gccatccggt agttcaccac ctcgaaaaag gccttctcgg ggtcgatgac ccggatgtac
     1261 acgatagcat ccacgttcgt tggcgagtta tccttcgtga taacttcctg tgatgggaca
     1321 tccatcacct gggtcctgag gtccatcttg attacgttgg tcacgagcgg tacaatccag
     1381 cggaacccag ggttcattct cccgatgtac ctgcccaggc ggatggcgag cccctgttcg
     1441 tagggctgga cgatcaccac cccccttgca aagatgatca cgattacaat gatcaaaaat
     1501 atggtaatga gtgtctcaat tacagccata gccttatttc acctcttcca cgatgatatg
     1561 gaccccttcc gaggccttta ccctaaccgt agcgccaggc tgaatctctc cggacccact
     1621 cctggcgctc cattcctggc cggcgatgag gactttgccg gagagcgaat ccgggaagac
     1681 cttcctgaca actctcccct ccatgcccac cagcgaatcg cggctgatgg tcgtgggggt
     1741 cgtctcgccg gggttgatcc ggcggtagag ccagatggtg atcatcgaga cggcgacagt
     1801 ggccaccacg ccggccacga gccccgtggg cccgctgaag atatccactc ccaggaggat
     1861 gagcaccccg aggatgatca tcactgtccc cggcaccgcg atgaagaatc ccgggctcga
     1921 agtctctatc accaggaaga gtgctccgag caccaccagg atccacccaa aggagaggtc
     1981 agccagctcg accataccca gggtatcgac ggccggggag aaaaaccctc tcgaagatga
     2041 ggaattggtg cgaaggccgg gcaaaggcag gcatgacact ttcgtcccct ccgtacttcc
     2101 tgctctcccg ggaaaaagaa cccaggagaa atacgcaaaa attaaaaatt ccgccacgca
     2161 aatgagttgg ttactatgag cacgtccatg ctggagaaga tacggggatt catgttcaac
     2221 ccttcacagg cgttccggag ggtaaaagac gaggaggcgg cagagaccgt caagtacctg
     2281 atgatcctgg cagtcttcta tgccatcatg agtgcgctgc tgacggcaat cgagatcttc
     2341 acccaccctt tcctcatggg gttgccctcg atgggcccaa acccggtcga gccgatcctg
     2401 tttgtctcgt ggattatcgt gatccttgtc gtcacacttg tcgttgcagt tatcttcggg
     2461 ttctggctgc acatatgggt gtaccttctc ggcgggagaa aaggtgtctg gcagactgag
     2521 aagagcgtct tttacaacct gaccccgact ctcctccttg ggtggatccc cgtgatcggc
     2581 cctctggtag gcggggtgtg gtcggtcatc atcggcgtca tcggggtcag ggatctgcat
     2641 aacatgccag atacgaaggc cgcgttcgcg gtggtgcttg caattgtcat cgcatccgtg
     2701 ctggcggtgc tcgttctcgg ggcaatgctt ctcgcggtgg tctcgtcaat cccgatgaat
     2761 gtgtaatgag cagttggggc taaagcccca actgcggggg gtatgcgggg gtggtccgga
     2821 ggggggaacc ccctccgttt attgaaacca gtacactttt ccactgtgtt gattcagacc
     2881 attcgtatca cacaatgaat gagcaggagt ggccctatgg ccactggtgc ggggggtatg
     2941 cgggggtaga ccggaggggg ggaccccctc cgggcacaag tcatgatata gtctcatcaa
     3001 gagttgaacc cgaattttgt atcacacaat gaatgagcag gagtggccct atggctactg
     3061 gtgcgggggg tgtgcggggg tggaccggag ggggggaccc cctccgttca ttgcgttcag
     3121 tacacttttc cactgcgttg atccagacca ttcgtatcat acaatgacag agcagttggg
     3181 gctttagccc caactgcggg gggtatgcgg gggtggaccg gaggggggga ccccctccgt
     3241 tcattgcgtt cagtacactt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 gaggggggga ccccctccgt tcattgcgtt cagtacactt ttccactgcg ttgattcaga
     3421 ccattcgtat cgcataatga cagagcagca gtggccctat ggccactgtt gcggggagca
     3481 ggagtgccat gaggcgctcc tgcgggggtt cacgcgggat actgctccat gaggattttc
     3541 tcactcgttg actatacggc cggatatccc tgtcataggg agagagtaaa aatgttcctt
     3601 ctgttcagtc attccgcgtc ctattgcgtt aaagggagga tccgcaatcc catcccacca
     3661 aaaaaagaga aacttccgac ctttacgccg gcttcgcgct ggaaaaaaac tcgtctcgca
     3721 cctgctcgag ctggtctttc tggaacttga tgttcatgct catgctccgg atggcaagcc
     3781 ctgcctcgtc ccccgtcttc atcttctcgt cactcacttt gtacgtgctc tcaagtatct
     3841 tgaggaggcg ggtgttgatg tcaatgctct tccgaaggcg cctgtacgtg tcgatggtct
     3901 ccttgtccac tcctgcttcg gtggccatca ggatatcgtt ctcgatcatc ctcctccggt
     3961 tgagctgccc ctcgatcgct tcatagagct ccttgtgggt gatgggcttc atgatataat
     4021 cctcgatgta gatgccgtac tcctgcgcct cgctcggggt gagctgcttt gcagtgagca
     4081 tcattacggg gatattcttc agatcggggg aattcttgat gttcatgagg gtctcccagc
     4141 catccatcgg ctccatcatg atgtcaagga ggatcaggtc gggcttgacg gtggagaggg
     4201 tatcgagcgc ctcctgtcca ccataggcgg ccacggtctg gtatccccct ctctgcagca
     4261 tggtcacgaa gatatcgaca ataaacgggc tgtcatcgat taccatcact gtgaacatta
     4321 aaatgacccc ccgatctccc cggccatggc ttcgagttgc cgtggtacag gggattgcct
     4381 gcccgcatcg gcgacgaccg cgatgataag gagtttctcc cctgccccga ttaccacgat
     4441 tgaggtctcc ctgatcacga tggataccgc accgggaggc ggcattttga ggatcgacga
     4501 tgtcgactcc gcggaggcaa agagcgtggc acacatggca gcaagccatg gttcattgaa
     4561 ctccccctga attgagttgc cgagcatgat cccatccctt gagatgagcg cacagtcaag
     4621 aactccttcg atccccttta tcctctcgat ataccccttt atcttttcct tcagcatatg
     4681 acctcaccc
//
LOCUS       DAOQYR010000136         4629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_18936_length_4629_cov_7.400306, whole genome shotgun sequence.
ACCESSION   DAOQYR010000136 DAOQYR010000000
VERSION     DAOQYR010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18936_length_4629_cov_7.400306"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PKM87_11215"
     CDS             complement(<1..423)
                     /locus_tag="PKM87_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNQ30679.1"
                     /translation="MIVLKGEYIFDHEMYSPMIVERKDPKWFLLEQVLAVVTTRRAQQ
                     EFAKYEITPINSAAEAVKIAIVAMFFSVDYAFVVQELKERKKLRRFMGIHAVPSVETV
                     YSILSKIDEYQFISLITGILNSCCKWKRRRGMNTFLVDA"
     gene            896..1288
                     /locus_tag="PKM87_11220"
     CDS             896..1288
                     /locus_tag="PKM87_11220"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNQ30680.1"
                     /translation="MKRVENGEIQGVIPATILNELLHRIMIAETVEKGYACTSQDAVR
                     VLKEDPAIIRHLTIAGNVYDSLPTIGFEMIENERGISGLMYHFSKELCLMAKDAAIVA
                     YAHTYTLAHIVTNDRDFQRVKWLTCWGP"
     gene            1602..2006
                     /locus_tag="PKM87_11225"
     CDS             1602..2006
                     /locus_tag="PKM87_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNQ30681.1"
                     /translation="MAHTSPPQLNKVTYHRISSDTQGGSRFDTVTVEQNLVRAAPPAA
                     PFYVSTDGVASKFRFYTFEPGWIGDWHPAPTRQFLALMTGAVEMETTDGTVRRFLPGG
                     LVLLEDTSGRGHLTRNVGDGYATYFVIPVPVV"
     gene            complement(2283..2972)
                     /locus_tag="PKM87_11230"
     CDS             complement(2283..2972)
                     /locus_tag="PKM87_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HNQ30682.1"
                     /translation="MTHEKTASALLKIYSTLLDQYGNRGWWPADTPLETIIGAILAQN
                     VSWSNARKAIENLKAAGLLDVQVLGDADPTEISRHIISSRYYNQKARRISAFMQFLYA
                     EYGGDVDQMSGDDLPRLRMKLHSLKGFGEETVDSILLYACNVPVFVVDAYTKRIFSRY
                     GFLEKNATYQEVQHFFTAHLPKEVPLYNDYHAQIVNLGHSICRTHQDCHLCPIRSIPP
                     GIACEYEKNME"
     gene            complement(3430..3663)
                     /locus_tag="PKM87_11235"
     CDS             complement(3430..3663)
                     /locus_tag="PKM87_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNQ30683.1"
                     /translation="MKTLIEVHKEGKYFVAVDLLTNVADQASSEEEAVQNLKKGLEEH
                     YRLLIELTPSNHKLTYLDIEVDTLVKNSSAVSS"
     gene            3989..>4629
                     /locus_tag="PKM87_11240"
     CDS             3989..>4629
                     /locus_tag="PKM87_11240"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNQ30684.1"
                     /translation="MDIPRIFTISESAHRIHNPFTPENLATLGAALRLEPGARILDLG
                     SGSGEMLCSWAREYGITGVGIDMSTLFTKQAKLRAEELGVADRVRFIHGDAAGYVADE
                     KVDVAACLGATWIGGGVAGTIELLEKSLRPGGIILIGEPYWLKVPPTEDVARGCEANA
                     LSDFLLLPELLASFGHLNYDVVEMVLADREGWDRYEAAKWLTMRRWLDANPDD"
BASE COUNT         1129 a         1190 c         1142 g         1168 t
ORIGIN      
        1 ggcatcgacc agaaatgtat tcattccacg ccttcttttc catttgcaac acgaattgag
       61 gatgcctgtg atgagagaaa tgaactggta ctcatcgatt ttactcaaaa tgctatagac
      121 cgtctccacc gacggaactg cgtggattcc catgaatctt cgcaatttct ttcgttcctt
      181 caattcctgg acaacaaagg cataatccac tgaaaaaaac atcgccacaa tggcgatttt
      241 caccgcttct gcagcggagt ttatcggcgt tatctcgtat tttgcgaatt cctgttgtgc
      301 gcgacgtgtt gttacgactg ccaggacctg ttccaaaagg aaccattttg ggtccttccg
      361 ctctactatc atgggggagt acatttcgtg atcaaatatg tattcccctt tgagtacgat
      421 caattttgcg gttcattcat ggttattttt gaattttgtg gtatttccag ttgtactttt
      481 atccgggctt tatgatatgt gtcccaaaag tagattggag tatgatttag cttgttttgg
      541 gtaatggatt cgtaaggggg ctaacactta tgtaacggga atgtcaacta cactatggaa
      601 ttacggaaat gccccctgtt gaagtgcatc ggaacgttga gaaaccactc ataaaaagcg
      661 caaaacgaaa gggtagtaca gtccttggga aggagctggg ctcccgcgct gagcaactct
      721 ctggcattct taaaataaaa gatccgcgga ttatcgaaga gatcgccgaa agtgatctcc
      781 ttgcatagtc ttcaagagat tcccctcgat accagggcaa tttttgatac aaatattctc
      841 gtctactggg tgaccgaccg atcatccctg gtatggaaaa ctctgtacag agtgcgtgaa
      901 acgagttgag aatggagaga ttcagggagt aatcccggca accattctga atgaactcct
      961 ccaccgtatc atgattgccg agacggttga gaagggttat gcatgcacct cccaggatgc
     1021 agtcagggta ttgaaagagg atcctgcaat aatcaggcat ttgactatcg cagggaatgt
     1081 atacgatagt ctgccaacga taggatttga gatgatcgag aatgaacggg gaatatcagg
     1141 cctcatgtat catttctcaa aagaactgtg cctcatggca aaggatgcgg caattgtagc
     1201 gtatgcacat acctacactc tcgctcatat cgtcacgaac gatcgtgatt ttcagcgggt
     1261 caaatggctc acatgctggg gtccttgaaa agagaagagt atccctgcat tctattccac
     1321 tctcctcctc tcacctgtcg ataccatcga catgtcccaa acatgtgtcg aagaaattga
     1381 aaaatttcga cacgttggtc ggccctcatc aaggagcctg atgagggatt gattgaacta
     1441 catccgttcc ttcccaattt tttcggatat cagaatgccg atcctctcta attccttcag
     1501 ataccgcgct tcagtctaca caacctttat ccatcatcat tctgtttgta ctttcacctt
     1561 gaggcatgct ctgcccgaat attttaagga ggaactaaaa catggcacac acatccccac
     1621 cccagcttaa caaggtaacg tatcacagaa tatcttcgga tacacagggg ggctcccgct
     1681 ttgataccgt gacggtcgaa cagaatcttg tgcgtgcagc accacccgct gccccgttct
     1741 atgtttccac ggatggagtc gcatcgaaat ttcgcttcta tacgtttgag cccggttgga
     1801 tcggtgactg gcaccctgcc cccacccggc agttcctggc cctgatgacc ggggcggtgg
     1861 agatggagac gaccgatggg acggtgaggc ggtttttgcc aggcggcctc gtcctcctgg
     1921 aggatacgtc cggcagggga cacctgacga ggaacgtcgg tgacgggtac gccacctatt
     1981 tcgtgattcc tgtccctgta gtgtgaaccg gggagatcat ctcacgcgtt gttggctgga
     2041 cgccgggatt ccctgacggt gggaagaatt ccacaatcaa gtacacgtct ttaaagaacc
     2101 atcccttcta tggtagttat gtaccaaatg tccactcttg gaggacatca catggtgaga
     2161 aaccattctt acaaaacgaa gggcgaaggg gtgttccagt caaccaggag ggttgggata
     2221 tctacgatac ttctcacgta agaaaaggca gaaagggtgg atgcatggac cgggaattct
     2281 ttttactcca tgtttttctc atattcacag gcgatacccg ggggtattga gcggatcgga
     2341 cacaggtgac aatcctgatg agtcctgcag atgctgtgtc ccaggttcac gatctgggcg
     2401 tggtaatcgt tatacaaagg gacctctttt ggcaaatgcg cggtaaaaaa atgctggact
     2461 tcctggtacg tggcgttttt ttccaggaac ccgtaccggg aaaagatcct tttcgtgtag
     2521 gcgtcgacca caaataccgg aacgttgcag gcatacagga gaattgaatc gacggtctct
     2581 tccccgaacc ctttgagtga atgcaacttc atcctcaatc ggggaaggtc atccccggac
     2641 atttggtcga cgtccccgcc atactcggca tacaaaaact gcatgaatgc agaaatcctc
     2701 cgggctttct ggttgtaata cctcgatgaa atgatgtgcc gggatatctc tgtgggatca
     2761 gcgtccccca atacctgaac gtccagcagt cccgcggcct tgagattctc tattgccttt
     2821 cttgcattgc tccacgacac gttctgcgcc aatatcgccc caatgatggt ctcaagggga
     2881 gtatctgcag gccaccatcc ccggtttcca tactggtcca acaaggtgga atagatcttc
     2941 agcaatgctg atgccgtttt ttcgtgtgtc atgtcatgcc cgcaggtgaa tgcatgtcgg
     3001 gaaacagccc tggatcactg gggtgccggg ttcctggaga atcctcccag cagtggggtt
     3061 cccctatgcc actgaaccgg ccaccgtgga aaccccatga gccaacgtaa cgccagagat
     3121 cgaagattct cgtcttctcc tctcaggtat tccccaaaat ccctaaaaag gtctttcctt
     3181 ttcaatccac acattggagc ttgactgtgg agtgccctct tcttgtggaa aaactattcc
     3241 ttcacgtgcc ggagaaattc ttccctgtca atgtttgcct gcctgaggat cgcgagcatg
     3301 gttccggtgg ctaactccga ttggttggga accgtgacga gccgccgtgt ttctggatgc
     3361 aggagatgaa tatggctgcc ggtctgtcgt acaggcgtat agccaaaatc tgcgaggatc
     3421 ctgataattt cacgagctga cagcagagga atttttgaca agagtatcca cttcaatgtc
     3481 gagatacgtc aatttatgat tgctgggagt aagctcgatt aaaagccggt aatgctcctc
     3541 aagacctttc ttgagatttt gcaccgcttc ctcttcagat gatgcctgat cggcaacatt
     3601 cgtaaggaga tccacagcta cgaaatattt tccttccttg tggacttcaa tgagtgtctt
     3661 catgttcaga tacaatcttt aggtagtttt atgagaataa gagtatgttg gtcacagtca
     3721 ggtacgtgtc ccagccggcg atcgccagta ccggggtgcc cctccgctcc cgccccatgc
     3781 cgatgtgaaa acccggagag caggtaaaag tctgggccgg gaaaccgcgc ccattatccc
     3841 cggattcaca acccccccat tcaccataat ccggctcatc cgactcgaaa ccttaaggct
     3901 atggtgttaa cagttacaac ctttctctat tacagtctct aaatgtatat tccagttata
     3961 ccacaattga gtaaaaggag cgtttacctt ggacatccca cgtatcttca ctatatcaga
     4021 aagtgctcac cgcatccata acccgttcac accagaaaat ctcgccactc tcggcgcagc
     4081 attgcgcctg gaaccgggtg ctcgtatact cgacctcggc agcggttcgg gcgagatgct
     4141 gtgcagctgg gcacgcgaat acggaatcac cggcgttggc atcgacatga gcacgttgtt
     4201 caccaagcag gcaaaactcc gtgccgaaga actcggcgtc gccgatcgag tcaggttcat
     4261 ccacggcgac gctgccggct acgtcgccga cgaaaaagtt gatgtggcag cctgtctcgg
     4321 tgctacatgg atcggtgggg gagtcgccgg caccatcgag cttctggaaa agagtctccg
     4381 ccccggcgga atcatcctca tcggcgagcc ctactggctg aaggtacccc caacagaaga
     4441 cgttgccagg ggatgcgaag ccaatgcgct ctccgacttt ctcctgcttc cagaactcct
     4501 cgcatcgttc ggccacctca actacgacgt ggtggaaatg gtgctggctg accgggaagg
     4561 atgggaccgg tacgaagcgg ccaagtggct caccatgcgc cgatggctcg acgcgaatcc
     4621 cgacgacga
//
LOCUS       DAOQYR010000137         4602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_19068_length_4602_cov_4.786453, whole genome shotgun sequence.
ACCESSION   DAOQYR010000137 DAOQYR010000000
VERSION     DAOQYR010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19068_length_4602_cov_4.786453"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PKM87_11245"
     CDS             <1..250
                     /locus_tag="PKM87_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30685.1"
                     /translation="PDEMTGIVTIRGRAENGEIVVSVEDDGIGLPEGFDWRDTPTLGL
                     HLVPALVRQLNGRIEQVGVERGTMFRVVIPGIVTGETS"
     gene            247..1227
                     /locus_tag="PKM87_11250"
     CDS             247..1227
                     /locus_tag="PKM87_11250"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ30686.1"
                     /translation="MKSTRILVVEDEQIVAEDLKMTLEGLGYIVAGIASSGENAIELA
                     GTEKPDLILMDIMLAGKMDGITAASEIRGSHDIPVIYVTAYADSTLLDRAKLTEPYGY
                     IVKPFNEREVQSNIEIALFKHRMEHEIKKRDAILLALGFGVEWFLRQFSDTHRITLRT
                     APDKWGYDFHPILEQIAIAMDLDRVQVLQFRQAGAPFELLDLITEWTMGATPYYLKGK
                     EGPVVALSEIGLSGSMQNLLEGTPVRIPLEETPVNRHGLLEQLRVHTAIVFPVFVQDR
                     IWGLVLFATGEMREFLGEEVEAMKIAVNIIGGAISLYVSAEDMQVGGPSR"
     gene            1340..3007
                     /locus_tag="PKM87_11255"
     CDS             1340..3007
                     /locus_tag="PKM87_11255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ30687.1"
                     /translation="MTTILITEDEQVVAEDLRITLEGFGYAVAGVAATGEAAIQVAEA
                     ARPDLVLMDIYLAGEMTGIEAAEVIRSRLDIPVIFLTAYSDSEIIEEVRKTGPYGYLL
                     KPFNEQEMYITIEVALFRHSMDRALRESEQRYRLFVEHFQGVAFRLSMDLEPVFYHGA
                     LSLITGYTEAEIKAGKPPWDTLVHPDDRGLYEGEMQKARTVPGYTNEFQFRIIHQQGA
                     IRWIQAFIQNVVDARNTPLFIQGSLYDITSKVEAEERLRALNEQLDDLVKKRTMDLET
                     SNRELYLKNRALQVTTAGTRALVVAGGEDELLAIITRIVASSGAYRALWIGTLDEHGT
                     VRSQAAFGDTSCSFPEPPYALDALPPCVQEALKKEVVTVMGECKDDCEHCTWNCMGRE
                     EQGLVGPLPSTTRMLGGIFAVLPPHLVPDAPEITLFRQMAQEISFVIGYLRAGDREKM
                     AFEHITRILEQLATLNDQIRNPLQGIVGYASLDEDPRSKKIIDLSLQINEIVEKLDKG
                     YLESKKVRSYLERHERISPAPSGKKEHAGPRRNAARASPYRYQEHRW"
     gene            3001..3366
                     /locus_tag="PKM87_11260"
     CDS             3001..3366
                     /locus_tag="PKM87_11260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ30688.1"
                     /translation="MVTIAIVDDDPYIVEVFAVTFEHAGHSVLPFTDPQEALEKLPHL
                     APDIILLDLMMTPITGLQFLDEWRKLESLRDVPVIILSAWDVSSEDREKYASVIARVI
                     RKPILPRALLETVRELCPG"
     gene            complement(3580..4197)
                     /locus_tag="PKM87_11265"
     CDS             complement(3580..4197)
                     /locus_tag="PKM87_11265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNQ30689.1"
                     /translation="MEREDLHEIFERIQTGIVIIDPLDHTILDVNPNAAALMGHKRDK
                     VVGRICHQFICPAEKGRCPITDLGQEIDNAERVLINRDAEKVPILKTATKARLGEKEV
                     IIESFIDNRDRKIAEERRCAMIGYIDETVMRIREPLLLVKQNLEDLAGRVDDIPAGSG
                     ILQAELQVEAQRLAQIFENLLDIQRAIAEGREEIPESYKAFITRE"
BASE COUNT         1005 a         1291 c         1388 g          918 t
ORIGIN      
        1 ccccgacgaa atgacaggga ttgtcaccat ccgtgggaga gcggagaatg gcgagatcgt
       61 ggtatctgtg gaggatgacg gcatcggcct tcccgaaggg ttcgactggc gtgatacgcc
      121 gaccctcggg ctccacctcg ttcctgcgct ggttcggcag ctcaacggca ggatcgagca
      181 ggtgggtgtg gagaggggga ccatgttccg ggtggtgatc cccgggatcg tgacgggaga
      241 aacatcatga aatcgacgcg gattctggtg gtggaggacg agcagatcgt cgcggaagac
      301 ctgaagatga ccctcgaagg cctggggtac atagtggcgg gcatcgcgag cagcggtgag
      361 aatgcaatag aactggccgg gacggagaag ccggacctca tcctgatgga catcatgctc
      421 gcagggaaga tggacgggat cactgcggca tcggagatcc gcgggagcca tgatatcccc
      481 gtcatctacg tcacggccta cgcagactcg acccttcttg acagggcgaa actgaccgag
      541 ccctacggat acatcgtcaa gcccttcaac gagcgcgagg tccagtccaa tatcgagatc
      601 gccctcttca agcacaggat ggagcacgag atcaagaagc gggatgccat tctccttgcc
      661 ctcggctttg gggtggaatg gttcctgcgg cagttctcgg atacccacag gattacactc
      721 cggacggctc cggacaagtg gggctacgat tttcacccca tccttgagca gatcgcgatt
      781 gcaatggacc ttgaccgtgt ccaggtcctg cagttcaggc aggcaggagc accgtttgaa
      841 ttgcttgacc tcatcaccga atggaccatg ggggcaaccc catactacct caaggggaag
      901 gagggtccgg tggtggccct ctcagagatt ggcctctcgg gatcgatgca gaatctcctt
      961 gagggaaccc ctgtcaggat accccttgag gagacccccg tgaacagaca cggtctcctt
     1021 gagcagctca gggtccacac ggctatcgtg ttcccggtct ttgtccagga caggatctgg
     1081 ggcctcgtgc tctttgcaac cggggaaatg cgagagttcc tcggcgagga ggtggaggca
     1141 atgaagatcg cggtaaacat catcggcggt gcgatcagcc tttacgtgtc tgccgaagac
     1201 atgcaggttg gcggtccctc cagatgatta ataacccttc aggggatact gctgacatat
     1261 cgcctcccgg tctcatccca cgtcccagga gcagtacggg agatccatcc cgacaggacc
     1321 atggagaaca ggtgcgaaaa tgaccacgat cctgatcacc gaagacgagc aggtcgttgc
     1381 cgaagacctg agaataaccc tcgaagggtt tggctatgcg gttgcaggtg tggcggcgac
     1441 cggtgaagca gcgatccagg ttgcggaggc ggccaggcct gatctcgtgc tgatggatat
     1501 ctaccttgcc ggtgagatga ccggcatcga ggctgcagag gtgatccggt cacgccttga
     1561 cataccggtc atcttcctga ccgcatattc agacagcgag atcatcgagg aggtcaggaa
     1621 gaccggcccg tatggatacc ttctgaaacc tttcaacgag caggagatgt atatcaccat
     1681 cgaggtcgcg ctcttccggc actcgatgga ccgggcactt cgcgagagcg agcagcggta
     1741 ccggctcttc gtggagcact tccagggggt agcattccgc ctctccatgg atctcgaacc
     1801 cgtcttctat catggggcgc tctcgctgat cacggggtat accgaggccg agatcaaggc
     1861 agggaagcct ccatgggata cgctcgtgca cccggatgat cgtggcctgt atgaagggga
     1921 gatgcagaag gcgaggacag ttcccggcta caccaacgaa ttccagttca ggatcatcca
     1981 ccagcagggg gcgatccgct ggatacaggc attcatccag aatgttgttg atgcaaggaa
     2041 cacgcctctc ttcatccagg gatccctcta tgatatcacc tccaaagtcg aggccgagga
     2101 gaggctccgg gccttgaacg agcagctcga cgacctggtg aagaaacgga ccatggacct
     2161 cgagacatcc aaccgagagt tgtacctgaa gaaccgtgca ctccaggtca ccacggcagg
     2221 tacccgggcg cttgtggtgg cgggaggcga ggatgaactg cttgccatca tcaccaggat
     2281 cgtcgcatca tcaggcgcgt accgggcgct ctggatagga acactcgacg agcatggcac
     2341 agtccgttcg caggctgcct ttggggatac ctcctgcagc ttccctgagc cgccgtacgc
     2401 cctggacgcg cttcccccct gtgtgcagga ggcactgaaa aaggaagtgg tcacggtgat
     2461 gggggaatgt aaagacgatt gtgaacactg cacatggaac tgcatgggaa gggaggagca
     2521 ggggctggtc ggaccgctgc cttccaccac ccggatgctg ggggggatct tcgccgtgct
     2581 cccccctcac cttgtgccgg atgcgcctga gatcacgctc ttccggcaga tggcgcagga
     2641 gatctctttt gtaatcgggt atctcagggc aggagaccgg gagaagatgg ccttcgaaca
     2701 tatcacgcgg atcctcgagc agcttgcgac cctgaacgac cagatccgaa acccgctcca
     2761 ggggatcgtc gggtatgcaa gcctcgacga agacccccgc tccaaaaaga tcatcgacct
     2821 ctccttgcag atcaacgaga tcgtcgagaa gctggacaag gggtacctcg agtccaaaaa
     2881 agtcaggagt taccttgagc gccacgagag gatctccccc gctccctctg ggaagaagga
     2941 gcacgcgggc ccgagacgaa acgctgccag ggcatctccg tatcggtacc aggagcacag
     3001 atggtgacca ttgccatcgt ggatgacgac ccctatatcg tggaggtatt cgcagtcaca
     3061 ttcgagcatg ccggccattc tgtcctcccg ttcaccgatc cccaggaagc gctggaaaag
     3121 ctcccgcacc tcgctccgga tatcatcctc ctcgacctga tgatgacccc catcaccggg
     3181 ttgcagttcc tcgacgaatg gcggaagctc gagtcgctgc gggacgtccc ggttatcatc
     3241 ctttccgcct gggacgtctc atccgaagac agggagaagt atgcatccgt catcgcccgg
     3301 gtcatccgta agccgatatt gccgcgggcg ctcctcgaga cggtaaggga actctgtccg
     3361 ggatagcgga agtgccggcc gctgccatgg tatcctgcag aaggatggat ttcatggccc
     3421 aaaggacaga agcgcgtggc aaggcatggc ccttcatagc actccattac cgcagtcccg
     3481 cgggatctgt ggggaaaatg aaccgggtgt cctcgaagag cgcacgggca cggaagcgat
     3541 tttgattagg aaacggggag acattcgcgg attgttcaat tactcccttg tgatgaacgc
     3601 cttgtaggac tctggaatct cctcgcgtcc ctctgcaatt gccctctgga tatcaaggag
     3661 gttctcaaag atctgcgcca ggcgctgtgc ctccacctgc aattccgcct ggagaatccc
     3721 ggaccctgca ggaatatcgt ccaccctgcc ggcgagatct tcgaggttct gtttcacaag
     3781 gaggaggggc tcccggatcc gcatcacggt ctcgtcgatg tagccgatca tcgcacacct
     3841 cctctcttct gcaatcttcc tgtcccggtt gtcgatgaaa ctctcgatga tcacctcctt
     3901 ttctcctaac cgtgcctttg ttgcggtctt caggatgggg accttctcgg cgtcccggtt
     3961 gataagtacc cgttctgcat tgtcgatctc ctggccaagg tccgtgatcg ggcatctgcc
     4021 cttctccgcg ggacagatga actggtggca gattctcccg acaactttat cacgcttgtg
     4081 tcccatcagg gcagcagcat tgggattgac gtcaagtatg gtatggtcca gcgggtcgat
     4141 gatcacgatc cccgtctgga ttctctcaaa aatttcgtga aggtcctctc gctccattcc
     4201 atgctccctt gagaggtatt ggtgggggga gaggtaatag tgctttgctt gagtaagaat
     4261 ggaaatgcac gttgccgcga ggacgtccgc aagggttgca gggaaaagat aaaatcaggg
     4321 gatgagcgag acacaggcaa tgcgttcccg gtgattcatg ggccgtcctg cgggggtgat
     4381 cccaccgcac aggtcacctc ccctcctgtc ccgggtcccc tccggattct caatagtgat
     4441 atgtggaaaa ggcctttatt cccgcgggct gaaaaggaca ttaccgtgag atccggaaac
     4501 acatggaaaa ggccgggtgg tgccagtcgg gagaaggatg ccatgaggag gaaaccatgc
     4561 ctgcaaagaa ggagatcata gaagagcttg gtgaggaggg gc
//
LOCUS       DAOQYR010000138         4526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_19526_length_4526_cov_9.766719, whole genome shotgun sequence.
ACCESSION   DAOQYR010000138 DAOQYR010000000
VERSION     DAOQYR010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19526_length_4526_cov_9.766719"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..794
                     /locus_tag="PKM87_11270"
     CDS             66..794
                     /locus_tag="PKM87_11270"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNQ30690.1"
                     /translation="MHTPRPVQALLIDMDNTLFDLVGAKHAACRAITEKTGVGDADLL
                     FSYFLRRTRGFEDPGNILDFLVDEGIYSDSLFDMCSALYRDVKLSHIEPYPGVQKTLK
                     RLVEREYPLALVTDAHRIDAIPRLEKAGLRDYFDQVVTYDMTWKKKPDPVPFLYALRR
                     MNTAPGDAMIVGDSPRRDIAPGKDLGMTTVYARYGDRFSSSRNKGRADFTIDEFAQLI
                     PLLSDLERPGKGRRSPCAAGSPSR"
     gene            767..1450
                     /locus_tag="PKM87_11275"
     CDS             767..1450
                     /locus_tag="PKM87_11275"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNQ30691.1"
                     /translation="MCGRFTITLTVGFGERFGVDESSLDLVPRYNIAPSQQVPVIFTT
                     PAGARAAMPMTWGLVPSWTRDMTGARPLINARADSLAERPAFRGPLARHRCIIPATGF
                     YEWQKSGTQKVPYYICRKDKGFLAFAGLYDVLRGRNPPLWTFAIITTEPNPLVARFHD
                     RMPAILTRDAEDRWLTPGPIAERDRASMLAPCADPILEAYPVSRAVNDPSRDGPDLIR
                     MSGDRTLDV"
     gene            complement(1473..2342)
                     /locus_tag="PKM87_11280"
     CDS             complement(1473..2342)
                     /locus_tag="PKM87_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNQ30692.1"
                     /translation="MEQTGEIIRDQQVQADGTAYRVVVREEDLSRFYPGMLRYTLEAW
                     AGPEVLAQFRTNTYEYSPAMPFHARQVAEERAASWEAELRADPGVFCESHPAPSLPGG
                     RVQDGRIVIIQGSPRPGGNSAILASWAAEAARREGREIEVIYPHDMDIHPCIGCYQCY
                     NTGTCVFQDDMNEIIDAVAKCRLLVICSPVYTNTVPAGLKALLDRFLALHAEMTFGGH
                     LRVRKGLLMAVAGRKGQDNFMCVTEVIRVFFSHLGITPLQPVLVDATDVIRDVTKVEG
                     LEDRVRYLVRENL"
     gene            2438..3085
                     /locus_tag="PKM87_11285"
     CDS             2438..3085
                     /locus_tag="PKM87_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HNQ30693.1"
                     /translation="MQGAREQEIQKLARLAAERGAEARVISSRDVIVSDWVRFKCRFG
                     CKGYGKHLSCPPYAPAPEETRRMVREYETGLLLRFEGIPGHRDLSPDQIPEDFHPFFR
                     DLILWVNGTVHFLEKTAFYDDFHKAFGFGAYPCIYCEHEHCVAEEQPGVVDESIRRMC
                     RHMDLVRPSMEAAGMDVFATARNAGWELHTVPCRDLEYGMMEHGNITSIGLVLLE"
     gene            3478..>4526
                     /locus_tag="PKM87_11290"
     CDS             3478..>4526
                     /locus_tag="PKM87_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNQ30694.1"
                     /translation="MKNPKYITSIEDLPGIPGDEKKRLAEVTKKFAFRTNEYYASLID
                     WDDPADPIRRIIVPDPRELEEWGALDPSREKDYTVAPGVQHKYRETALFLTVDVCGGF
                     CRFCFRKRLFMNRADEIARDFSAGIEYVRQHPEITNVLLTGGDPLLLATSRLERIISQ
                     IREIDHVQIIRIGSKMAAFNPYRILNDPDLPGLIKTYSTPKKKIYLMAHFNHPRELSP
                     QAIDALEVLRDAGAIVVNQTPLLRGINSDPAVLTELFRKLSFIGVPPYYVFQCRPTLG
                     NHLFEMPVEESYLIFEKARACCSGLAKRARFVMSHATGKIEVVGKTEAATYFKYHQAA
                     DPRDVGRFLAFKCNP"
BASE COUNT         1008 a         1327 c         1265 g          926 t
ORIGIN      
        1 tttcgcatgt tgccaaacaa tacttccata agggacaaaa gagacctctt taatcagtga
       61 gaggaatgca tacaccacgt cctgtccagg cactgctcat cgacatggac aataccctct
      121 ttgaccttgt cggcgcgaag catgcggcat gccgtgcgat caccgagaag acaggagttg
      181 gagatgccga tctgctcttc tcgtattttc tccggcggac aaggggattc gaggaccccg
      241 ggaatatact ggacttcctc gttgatgaag ggatatactc ggattccctg ttcgatatgt
      301 gcagcgcctt gtaccgcgac gtgaagctct cgcatatcga gccttacccg ggagtccaaa
      361 agacactgaa gcgccttgtc gaaagggaat accccctggc cctcgtcacc gatgcccaca
      421 ggattgacgc gatcccccgg cttgaaaagg ccggcctgcg cgattatttt gaccaggtgg
      481 tcacttacga catgacatgg aagaagaagc cggaccctgt gccattcctt tatgccctcc
      541 ggaggatgaa taccgcgccg ggagacgcca tgatcgtggg ggacagcccg agaagggata
      601 tcgcgccggg aaaggatctt ggaatgacca ccgtgtatgc ccgctatggg gaccgtttct
      661 catcctcgag gaataagggg cgtgcagatt tcaccattga cgagttcgcg cagctgattc
      721 cgctgctatc ggatctggaa agaccaggaa aagggaggag atcgccgtgt gcggccggtt
      781 caccatcacg ctgacggtag gattcggcga gcggttcggg gtcgacgaat cttccctcga
      841 tcttgtcccg cgttacaaca tcgcaccctc ccaacaggta ccggttatct tcaccacccc
      901 ggcaggagcc cgggcggcta tgccaatgac ctggggactt gtcccctcat ggacgcgcga
      961 tatgaccgga gcccgcccac tgatcaatgc acgggccgac tcactcgcgg agcgccccgc
     1021 attcaggggg ccgcttgcac ggcaccgatg cattatccct gcaaccggct tctacgagtg
     1081 gcagaagtcc ggaacccaga aagtgcccta ctacatctgc aggaaggaca agggtttcct
     1141 tgcctttgca gggctgtatg atgtcctcag aggtcgaaat cctcctctgt ggacgtttgc
     1201 aatcattacc acggaaccca acccgcttgt ggcacggttc cacgaccgga tgcctgccat
     1261 cctcacgcgg gatgccgagg accgctggct gacacccggc cccatcgcgg agcgggaccg
     1321 tgcatccatg ctcgcaccct gcgccgatcc cattcttgaa gcataccccg tgtcgagggc
     1381 agtgaacgat ccctcccggg acgggcctga tctcatccgg atgtcggggg atcgcacgct
     1441 cgacgtgtga cacgaccttc ctggtatttt tcttacagat tctcccttac taagtacctt
     1501 acacgatctt cgagaccttc caccttcgtc acatcgcgta tcacgtctgt tgcatcaacg
     1561 agcacgggtt gcaggggagt tatgccaaga tgggagaaaa acaccctgat cacttctgtc
     1621 acgcacatga aattatcctg gccctttcgt ccggcaacgg ccatcagcag tcccttccgc
     1681 acacgaagat ggcctccaaa ggtcatttct gcgtgcaagg ctaaaaaacg gtcaagaagg
     1741 gctttcaggc ccgcaggaac ggtgttggta tacacaggcg agcagatgac gagcagtctg
     1801 cactttgcga ccgcatcaat gatctcattc atatcatcct ggaagacgca ggtaccggtg
     1861 ttgtagcact ggtagcaccc gatgcagggg tggatgtcca tgtcatgggg gtaaatgact
     1921 tctatttccc tcccctcgcg gcgcgcagcc tccgcagccc atgatgcaag gatggcgctg
     1981 tttcctcccg ggcgggggct tccctggatg atcacgatac gcccgtcctg tacgcgcccg
     2041 ccaggcagtg aaggtgcggg atgtgattcg cagaatacac caggatcggc acgcaactcc
     2101 gcttcccatg aggcggccct ctcctctgca acctgcctgg catggaaagg catggcgggt
     2161 gagtattcgt aggtgtttgt ccggaactgc gcgagaacct ccggcccggc ccaggcctcg
     2221 agggtatacc tcagcatccc aggatagaac cgggagagat cctcctcacg gacgacgaca
     2281 cgatatgcag ttccgtccgc ctgcacctgc tggtcccgga ttatctctcc ggtctgctcc
     2341 atgaaagagt tgtaggagtg gtgagggata tacgttccag tcggaagtga tacttttatg
     2401 ggacggaacc tgaagaattc tccactggga atgtgcaatg cagggggcgc gtgaacagga
     2461 gattcaaaaa ctggcccgcc tggcggcgga gagaggggcc gaagcccgcg tcatctcctc
     2521 gcgggacgtc atcgtctctg actgggtccg gttcaagtgc cgtttcgggt gcaaggggta
     2581 cggaaagcac ctgagctgtc cgccgtatgc accggcacct gaggagaccc ggcggatggt
     2641 cagggaatac gaaaccgggc ttctcctcag attcgaaggg attcccgggc acagggatct
     2701 ctccccggac cagatcccgg aagattttca cccgttcttc cgcgatctta tcctctgggt
     2761 gaacgggacc gtccatttcc ttgagaagac tgcattttat gacgactttc acaaggcgtt
     2821 cggcttcggc gcatacccgt gcatctactg cgagcacgag cactgcgtcg cagaggaaca
     2881 accgggggtc gttgacgaga gcatcaggag gatgtgccgg cacatggacc ttgtccgccc
     2941 cagcatggag gctgccggca tggatgtatt tgcaaccgca agaaacgcag gctgggaact
     3001 gcacaccgtt ccctgcaggg acttggaata cggcatgatg gaacacggga acatcacctc
     3061 gattggcctt gtgctcctgg aataggtgcg gttctccatt tgctgatatc ctccttgtga
     3121 gacggggaat gagaatggct atcccgcttc tgccgaaggg aaaatgactg cgaagaacat
     3181 ccaggatgcg gggtaaagag gccggcgagg ctccgcggaa tcgttcatcc actcatgcca
     3241 gcactgtccc tctgccgcgc ttgatgctgc ccccaaaaaa atccctggca aaattcgatt
     3301 ttttattgac ctgacctaaa actataaata tattgaatag acataataaa gcgcactttc
     3361 tacccccact gggcccccat ccctgcaggc aataactttt actggtcgca tcctgcagcg
     3421 gaacttcaac accagagggc cagaaccctg gggggtgaga tcccgcagga gcagacaatg
     3481 aagaacccga agtacatcac ctcaattgaa gatctccccg ggatcccggg agatgagaag
     3541 aagagacttg cggaggtgac gaagaaattc gccttccgca caaacgaata ctatgcctcg
     3601 ctgatcgact gggacgaccc cgcagatccc atcaggagga tcatcgtccc tgatccacgc
     3661 gagctcgagg aatggggggc ccttgacccc tcgagggaga aggactatac tgtcgcgccg
     3721 ggcgtccagc acaagtaccg cgagacggcg ctcttcctca cggtcgacgt ctgcggcggg
     3781 ttctgcaggt tctgtttcag gaagaggctc ttcatgaaca gggcagacga gattgcgcgc
     3841 gatttctcgg cgggcatcga gtatgtgcgc cagcacccgg agatcaccaa tgtcctcctt
     3901 acagggggag atcccctgct tcttgccacg agcaggcttg aacgcatcat ttcgcagatt
     3961 cgtgagatcg atcacgtcca gattatccgc atcggaagca agatggccgc gttcaacccc
     4021 tatcgcatcc tcaacgatcc tgaccttccg gggctgatca agacctacag cacccccaag
     4081 aagaagatat acctgatggc acacttcaac cacccgaggg agctctcacc gcaggcgatc
     4141 gatgcgctgg aagtcctccg cgacgcgggc gcgatcgtcg tgaaccagac tccgctcctc
     4201 cggggaatca acagtgatcc cgcagtgctt acagaactct tcaggaagct atcttttatc
     4261 ggggtcccgc cctactatgt cttccagtgc cgtccaaccc tcggaaacca cctcttcgag
     4321 atgcctgtcg aggaatcata cctcatattc gagaaggcga gggcgtgttg ctcgggcctt
     4381 gcgaagaggg cgagattcgt gatgtcccat gccaccggca agatcgaggt ggtcggaaag
     4441 actgaagctg caacatactt caagtatcat caggctgccg atccacgcga tgtaggcagg
     4501 ttccttgcgt tcaaatgcaa ccccga
//
LOCUS       DAOQYR010000139         4506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_19622_length_4506_cov_8.375871, whole genome shotgun sequence.
ACCESSION   DAOQYR010000139 DAOQYR010000000
VERSION     DAOQYR010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19622_length_4506_cov_8.375871"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(341..1249)
                     /locus_tag="PKM87_11295"
     CDS             complement(341..1249)
                     /locus_tag="PKM87_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNQ30695.1"
                     /translation="MLKLSQFRPVTIRDQALFRAHYSRYPQVHSDNLFSNMLCWNHFA
                     NYAYLQVGGAIVLCSTIQGKTRFRPPIGPRDPDLLEEVIHLAIREGDEAPLVLVDTPA
                     REWIQYLYPELPLHEDRDHFEYVYCAEDLARLPGGEYLTLRRQLNRFRKQCRPLLEEI
                     APHNLADVERFLHEWCEWKQCDEHPFLAEEKDAVLYAISHFTELGLSGLLIRIEGKVG
                     AIAVFEEMNRETAVVHFEKGLPDCEGIYKAINAETAAFLEERYRYINRESDMGVPGLR
                     EAKMRYHPHHMVEVSYALHDDLARMV"
     gene            complement(1243..1683)
                     /locus_tag="PKM87_11300"
     CDS             complement(1243..1683)
                     /locus_tag="PKM87_11300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ30696.1"
                     /translation="METNDEELDLFVVREWDSRALVELYRAGGWWKEEWDPADIPLLV
                     RSTFAFVVAVHRQSGRTVGMGRAISDGVSDAYIQDLVVLPEFRGMGVGKAILERLVGI
                     CKERGVCWIALVAEPGTSDFYESIGFEAMEGHIPMKFRGGENRC"
     gene            complement(1745..2584)
                     /locus_tag="PKM87_11305"
     CDS             complement(1745..2584)
                     /locus_tag="PKM87_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ30697.1"
                     /translation="MRLRVLADNSTLIDRYYLAEPGFSALVETEGGKVLFDLGYSDVF
                     QKNAMKMGQSLLDLDYVVLSHGHLDHTWGLFHLVMALTEAQIEKREFKRPVLVAHPEV
                     FLTKQSGKVPESGALISEEKCGEHFGLHLSREPFWLWDDLVFLGEIPRRFKFEPPEPW
                     RKRRRNGGIEPDMMPDDSALAWKGEEGLVVITGCSHAGICNIVAYAREVCGEERVQDI
                     VGGFHLMKAPQSRLDAMASALKEMGVNRMHPCHCTGFRAVHRLAREFQVEETGSGLVC
                     EYR"
     gene            complement(2769..3314)
                     /locus_tag="PKM87_11310"
     CDS             complement(2769..3314)
                     /locus_tag="PKM87_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ30698.1"
                     /translation="MRITVSGLPGSGTTSLARHLASTQNYTLISAGEVFRQMAGEKGL
                     DIAEFGRIAEKNPSIDREIDARQREIAEARDDIIAEGRLSGWCVRNADLRIWLQAPME
                     CRVERIFSRDTIVDIDTALSLTREREASEARRYREYYSIDINDLSLYHMVLNSAKFGV
                     EDLSSLVETAISRIEQAKTVS"
     gene            complement(3314..3907)
                     /locus_tag="PKM87_11315"
     CDS             complement(3314..3907)
                     /locus_tag="PKM87_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EMC3/TMCO1 family protein"
                     /protein_id="HNQ30699.1"
                     /translation="MKKKFGGFIPLLVAMAMILSYSIAPIRDGVGAALDLVLGPLAGM
                     MPFYILIILLSAATGLYSSIIQKYTIDYERMTDVQERMKVFQREFREAQLSGDEKKIK
                     RLDAKRDRVMKEQMELSQQQFTPMAYILIITVPIFFWLLFRLGVAPDATVVMPFFGTH
                     TLTDAVLGPVPAWMLWYMICSLSISQVVRKSLNIGGI"
     gene            complement(3914..4486)
                     /locus_tag="PKM87_11320"
     CDS             complement(3914..4486)
                     /locus_tag="PKM87_11320"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNQ30700.1"
                     /translation="MTGRRVIVTGVPGVGKTTVVTGALKVLEGEGITYRSLNFGTYMF
                     ETAQKEGFAKDRDEMRRLPGDVQKKLQQSAARAMAKESGNILVDTHASVRTPKGLLAG
                     LPEWVLRELMPDTIILVETDEDQILMRRLSDETRTRDMEGSRAIGDHQQFNRAIAASY
                     SMLTGCTIKYITNADFLLDRAIEEMAAVLR"
BASE COUNT          945 a         1324 c         1222 g         1015 t
ORIGIN      
        1 taattctccc accatagttc ccttggaact actatcttag aggttaaatc tccctatttt
       61 aaagctttgc cttattccac acgcgatacg ctctcctttt ccaataatct gggcaaatgt
      121 tgagcctttt tcggaaaaat atgacaaaat cagtcattat tggaaaaagg cacagaattg
      181 cttcaattcg taacgcgcac gtcccatatt tgagggaaaa ataaggccat aaaagccata
      241 ttttttcatg ctatattatc gggaataata taatgtttag tatttttaaa aagaagcctg
      301 attccgccgc gcaggagaga gtgaaggcgt tgcaagaggt tcacaccatg cgggccaggt
      361 catcgtgcaa tgcatatgag acctcgacca tgtggtgagg gtgataccgc atcttcgctt
      421 cacggaggcc cgggaccccc atgtcgctct cacggttgat gtagcgatac ctttcctcaa
      481 ggaatgccgc ggtctctgca ttgatagcct tgtaaatccc ctcgcagtcg gggaggcctt
      541 tttcgaagtg gactactgcg gtctcgcgat tcatctcctc gaagactgct atcgctccca
      601 ccttgccttc gatccggatg aggaggccgg agagcccgag ctccgtgaag tgcgagatag
      661 catacaggac ggcgtccttc tcttctgcga ggaaggggtg ttcatcgcat tgtttccact
      721 cgcaccactc gtggaggaac ctctccacgt cagcgagatt gtgcggtgcg atctcctcca
      781 ggagcggcct gcattgcttc ctgaaacggt tcagctgccg ccggagggtc aggtattctc
      841 ccccgggcag gcgggcgagg tcctctgcac agtatacgta ttcaaagtga tcacgatctt
      901 cgtgaagcgg cagttcagga tacaggtact gtatccactc cctcgcaggc gtgtccacca
      961 gcaccagcgg cgcctcgtcc ccttcccgga ttgcaaggtg tatcacctct tcgagaaggt
     1021 cgggatcgcg gggaccgatg gggggacgga accttgtctt gccctggatc gtgctgcaga
     1081 gcacgatcgc accccccacc tggagatacg cataattagc aaaatggttc cagcagagca
     1141 tgttcgagaa gaggttgtcg ctgtgcacct ggggataacg ggagtagtgg gcccggaaga
     1201 gtgcctgatc cctgatggtg acggggcgga actgcgagag cttcagcacc tgttctcccc
     1261 tccgcggaat ttcatgggga tatgtccttc catggcctca aatcctatgc tctcataaaa
     1321 gtccgaggtc cccggctcgg ccacaagggc aatccagcag actccccttt ctttgcagat
     1381 cccgaccagc ctctctagga tagcttttcc cacccccata cccctgaatt ccggcagcac
     1441 gaccaggtcc tggatgtacg catccgagac accgtccgag attgcccggc ccattcccac
     1501 cgtccggccg ctctgcctgt gaacggcaac gacaaatgca aaggttgacc tcaccaggag
     1561 cgggatgtct gcgggatccc attcctcctt ccaccagccg cccgcgcggt aaagttcaac
     1621 gagcgcccgg gaatcccatt ccctgaccac gaagaggtcc agttcttcat cattcgtctc
     1681 catgtctgcc cacctgtccc aaaaatgaga agtgattgct ctcttcagtg atgaaaattt
     1741 ttacctatct gtactcgcag accagccccg atccagtctc ctcaacctgg aattcacggg
     1801 caagccgatg gaccgctctg aacccggtac aatggcaggg atgcatgcgg ttgacaccca
     1861 tttctttcaa cgcgctggcc atcgcatcaa ggcggctttg cggggccttc atcaggtgga
     1921 acccgccgac gatgtcctgc acgcgctctt caccgcagac ctcgcgtgcg tatgccacga
     1981 tgttgcagat gcccgcgtgg gagcacccgg tgattactac cagcccctcc tcccctttcc
     2041 acgcgagtgc gctgtcgtcc ggcatcatgt cgggttcaat gccgccgttg cgtctcctct
     2101 tcctccaggg ttcaggcggc tcgaacttaa acctccgcgg tatctcccca agaaacacaa
     2161 ggtcatccca gagccagaat ggttcacggg agaggtggag gccgaaatgc tccccgcact
     2221 tctcttcaga tatgagtgcc cctgattccg ggacctttcc ggactgcttg gtcaggaaga
     2281 cctcgggatg ggcaacgagg acaggccttt tgaactctct cttctcgatc tgggcctctg
     2341 tgagtgccat cacgaggtga aacaggcccc aggtatgatc gaggtggccg tgggagagca
     2401 cgacgtagtc gaggtcgagg agggactggc ccatcttcat ggcgtttttt tgaaagacat
     2461 cggagtatcc gagatcaaag agcacttttc ccccctccgt ctccaccagc gcagagaacc
     2521 caggctcggc gaggtagtag cgatcgatga gggtggagtt gtctgccaat acccggagtc
     2581 tcattgggcg cttcctcctc caggaatgat catgccactc cgcttccgca tggatatact
     2641 atctttccgt aatcgacgat aatatggccg catgttcacg caaaagacgt tattcagaat
     2701 gatggctgag gcaatcggcc aggataaaag tttagaggag gagagagtgt gttgtggtat
     2761 gcgtaagttc aggagaccgt ctttgcctgc tcgatcctgc tgatggcggt ctctacgagg
     2821 gaagaaaggt cctccactcc gaactttgcc gagttgagga ccatgtgata gagggagagg
     2881 tcgttgatgt cgatcgagta gtactccctg tagcggcggg cctccgatgc ctcccgctca
     2941 cgggtcagcg aaagggcggt atcgatgtcc actatggtgt cgcgtgagaa tatccgttca
     3001 accctgcact ccatcggcgc ctggagccaa atccttaggt cggcgttgcg cacacaccac
     3061 cccgagagcc ttccctcggc gataatgtcg tcccgcgcct cggcgatctc gcgctggcgt
     3121 gcatcgatct cgcggtcgat cgagggattc ttctcggcga tccttccgaa ctcggcaatg
     3181 tcaagcccct tttcgcccgc catctgcctg aagacctctc cggcagagat aagggtgtaa
     3241 ttctgtgtgg acgcgaggtg gcgggcgagc gaggtggtcc cgctcccggg aagcccgctc
     3301 accgtgattc gcatcaaatg cccccgatgt ttaaggactt cctgacgacc tggctgatcg
     3361 agagggaaca gatcatatac cagagcatcc acgccgggac cgggccgagc accgcatcag
     3421 taagcgtgtg agtcccgaag aatggcatga ccacggttgc atcgggtgca accccaagcc
     3481 tgaagaggag ccagaagaag atcggcacgg tgatgataag gatgtatgcc atgggagtga
     3541 actgctgctg cgagagctcc atctgctctt tcatcacgcg atctctctta gcatccagtc
     3601 tcttgatctt cttctcgtcg ccggagagct gggcctcgcg gaactcccgc tggaagacct
     3661 tcatcctctc ctgcacatcg gtcatccgtt cgtaatcgat cgtatacttc tggatgatcg
     3721 aggaatagag gccggtggcc gcggagagga ggataatcag gatgtagaac ggcatcatcc
     3781 ctgcaagggg tcccaggaca agatcgagcg ctgctcccac gccatcgcga atgggggcga
     3841 tgctgtagga gaggatcatt gccatggcca ccagcagggg aataaagccc ccgaacttct
     3901 tcttcatccc ggatcacctg aggactgccg ccatctcttc aattgcacgg tcgagcagga
     3961 aatccgcatt ggtgatatac ttgatagtgc acccggtcag catggaatag gacgcggcaa
     4021 tggcccggtt gaactgctgg tggtctccaa tcgctctcga accctccatg tcccttgtcc
     4081 gggtctcatc cgagagccgg cgcatcagaa tctggtcttc gtcggtctcg acgagaatga
     4141 tggtgtccgg catcagctcc ctgagcaccc attcgggcag acctgccagt aacccctttg
     4201 gtgtcctgac agatgcgtgg gtgtcaacga ggatatttcc gctctccttt gccatggcgc
     4261 gggcagcact ctgctgaagc ttcttctgca cgtccccggg gagccggcgc atctcgtcac
     4321 ggtccttcgc gaatccctct ttctgggcgg tctcgaacat gtaggtcccg aaattgaggg
     4381 atcggtaggt gatcccttcc ccttccagga ctttcagcgc accggtgacg acggtggtct
     4441 tgccaacccc cgggacaccg gtcacgatca ctctccttcc agtcattctc actccttccc
     4501 gaaaaa
//
LOCUS       DAOQYR010000140         4358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_20552_length_4358_cov_9.665582, whole genome shotgun sequence.
ACCESSION   DAOQYR010000140 DAOQYR010000000
VERSION     DAOQYR010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20552_length_4358_cov_9.665582"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..1563)
                     /gene="ftsA"
                     /locus_tag="PKM87_11325"
     CDS             complement(214..1563)
                     /gene="ftsA"
                     /locus_tag="PKM87_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F390 synthetase"
                     /protein_id="HNQ30701.1"
                     /translation="MSPGAFFDHHMETLPRDELDALVDERIKYTVRYAHDHSPFYRKW
                     FRDHGIRPEDVKSHDDLKSLPIISGRTIRENQPPQKNEFLFRSVPWEEIFTVHETSGT
                     SGTPKSFFLTWEDWERYAAKYARIFASQGFGKGDRVVICASYGMNVGANTMTLAARGI
                     GMTIIPTGKCTFPRRILESYRPTGIVGSVFKLLRLARQLEAEGLDPRESSLERLIVGG
                     ESFADESRAYVAEVWGREVYNTYGSTEGTMCGECSERSGLHVPEDLVHLDVYDPAMGG
                     FVPDGECGRAVLTTLIPPGGRCGTLLINYDTEDTTVVMTRAQCACGRTHMKIYTPQRE
                     AETLWILGTPVNRVDIERGVFSPENMEYLTGEYEAFVYPCDDGGVILRVSMEAQDPGH
                     LPREEIGERFLSSFLRAKPGLAPATSQDFQVLFHFAPPGGLELSRVKGRPKRFVDRR"
     gene            1765..2151
                     /locus_tag="PKM87_11330"
     CDS             1765..2151
                     /locus_tag="PKM87_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNQ30702.1"
                     /translation="MAGTKRYKCKVCGYIYSPVRGEPHNGIPAGTDFEDLPDSYVCPI
                     CGMEGKGKIGKWGFEEWVPTKWICSVCGYVYDQRRGEPHRGIKPGTAFEDLPDDYTCP
                     VCNLDPKISREYGKVFKQGFDPLDLP"
     gene            2934..4274
                     /locus_tag="PKM87_11335"
     CDS             2934..4274
                     /locus_tag="PKM87_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNQ30703.1"
                     /translation="MFERILVPTDFSWYAQRTVRYATEIPGATGVILLHIAEDAGECM
                     TWPSLMEARNAPCTPANALAYAGDALEKAGLSVTYEVAPPKDAGTHAAILACAEQHKA
                     DLILIGGKGRGFFKDMLLGSVSRRVIEEAKTHVLVTHFLEQPGHGNEAPDVPVATGRP
                     LFSRILVPVDFSRPTYETIEFLSDVGGYDEIILMHVIASVEDRHDLQKKLQHSMQALS
                     YLQRDLALGGGKIIPLIRFGDTVGEICRMADEERATLILISRFGASDYMKDGSIGSIA
                     EEVTKRARVPVLVRYPGSSLDVQVREVGADEFPRAGEVWAHYRQQHADPSTDRIFGVF
                     VQGDPVSLARCRRFPDGLEVDGVFTLEGFRRRGYARGAVSLLVRECGTEPLYTLSTAD
                     MIEFFRSFGFNEIPEKEMPGSIRERYSFALGDLAGADLYPMSRSAKGASEAPAG"
BASE COUNT          947 a         1223 c         1262 g          926 t
ORIGIN      
        1 gatagggggg ttgatgccag attctggggg tgtccggcaa aatgaaaaaa ccaggtgaca
       61 tagccagtga gagcagtatc atatccacgt atgagcttaa aaattttcat attccacccg
      121 ggctgggaga aggaatagct ggggagccag tgtttttcag agaaccttcg atgccgtaag
      181 atcctgtaac ccgcttgagc ctggcgacgc tttttaccgg cggtctacga aacgtttcgg
      241 ccgccctttc acccttgaaa gttcgagacc cccggggggg gcaaagtgaa agagtacctg
      301 gaaatcctgt gaagtggccg gtgcaagacc cggcttcgcg cggaggaacg aggagaggaa
      361 ccgctcgcct atctcctccc tgggcaggtg cccggggtcc tgtgcctcca tgctcacccg
      421 gaggatgaca cctccatcat cgcaagggta gacaaatgcc tcgtactccc ctgttaagta
      481 ttccatgttt tcgggcgaaa agaccccgcg ctcgatatcc acccggttca ccggggtccc
      541 caggatccag agggtctctg cctcccgctg gggggtgtaa atcttcatgt gtgtcctgcc
      601 gcaggcacac tgcgcgcgcg tcatcacgac agtcgtgtcc tcggtgtcgt aattgatgag
      661 gagggtaccg caacggcctc ccggagggat gagtgtcgtg agtactgccc gaccgcattc
      721 accgtcgggg acgaatcctc ccatcgcagg gtcgtacacg tccaggtgaa ccaggtcctc
      781 cggcacgtga aggccggaac gctcgctgca ctcgccgcac atggtcccct cggtgctgcc
      841 ataggtgttg tagacctcgc gcccccatac ctccgcgacg tacgcccgcg actcgtcggc
      901 aaaactctcc ccgccgacga tcagccgctc gagcgagctt tcccgcgggt caagaccctc
      961 ggcctccagc tgccgcgcga gccgtaaaag cttgaagacg ctccccacga tcccagtagg
     1021 ccggtaactc tcaaggatcc tccgcgggaa tgtgcatttc ccggtgggaa tgatggtcat
     1081 cccgatcccc ctcgcagcga gtgtcatggt attggccccg acgttcatcc cgtaggacgc
     1141 gcagatcacc accctgtccc cctttccaaa tccctgcgat gcaaatatcc tcgcatactt
     1201 cgcggcatag cgttcccagt cctcccatgt gaggaagaag ctcttgggag tgccggaggt
     1261 accgctggtc tcgtgcaccg tgaagatctc ctcccagggc acactcctga agaggaattc
     1321 gttcttctgc gggggctggt tttcgcggat ggttctcccc gatatgatgg ggagtgattt
     1381 caggtcatcg tgggacttca cgtcttcagg gcggatccca tgatcgcgga accatttccg
     1441 gtagaacggt gaatgatcgt gtgcatacct gactgtgtat tttatcctct catccacaag
     1501 ggcatcgagt tcatccctgg ggagggtctc catatggtgg tcaaagaatg caccgggcga
     1561 catgatgtga gttctctgta taaaggatga ataaatctcg cttcagggct gttctgttcc
     1621 tgacgggaat acccggaata atccggggtc ccccgcatgc gacctggata cgagaattca
     1681 caaagggaac atgcagattc caggaaaaat agtattaaat cctgccaggg catcacattg
     1741 caatataagg gaagatgtgt gatcatggcg ggaacgaaac gctataaatg caaggtctgc
     1801 ggctacattt actcccccgt gcggggagaa cctcacaacg gcatccctgc agggacagat
     1861 tttgaagact taccggacag ctacgtctgc cccatctgcg gaatggaggg gaaggggaag
     1921 atcggaaagt ggggattcga ggagtgggtt cccaccaagt ggatctgctc tgtctgtggg
     1981 tatgtatatg accagaggcg gggtgaaccc caccgcggta tcaagccggg aactgccttc
     2041 gaggacctcc cggatgacta cacctgcccg gtgtgcaacc ttgacccaaa gatttccagg
     2101 gaatacggaa aggtgttcaa gcaggggttc gacccgctgg accttccgta aaacatctct
     2161 gaatcatttt ttccaaaagc gccagtaatc cttgaaaaac cggtaccctg gcatatgagc
     2221 atcacttttt tcgaatgaaa actgcacccg cgattttcat ctgtattgcg tgtgcctcga
     2281 agagaatcat gtagtttttt ttaatggttc ttcgctatct ggtgtgggta gaattggtga
     2341 accggacaca ttttgtgggg gctgtctgcc cctacacccc catgtgcgat gcacgctgcc
     2401 gcccccgaac ataccgcggg gcttccgccc tcgacccttg gcgacctgcg gtcaccttca
     2461 gatttagggc cactcctgag taatctggga ggagttctgt gaaaaagcac ggctgcggtt
     2521 ggagtgactg aattgatatg cagataacaa ccccgattca gtctccccac acaaaacagc
     2581 gaggaggctt tttaaattga aaatggctcc catggaagca catcccgcaa taggtagtgg
     2641 ccggaactgc aagggagggg caatgacgga tatcattcac agcatttcac aggaatgagg
     2701 agttgttccg ggactggggg ggcgccgcgg ctcccggaga tgccctgtct ggcaggaacg
     2761 gagaaggact ccccctttgt gtatcgcatc atatgatata tggttgaaaa aaccggcttg
     2821 tggatggata caagaatact gtccggttgg cacgggtgaa ccccgggata tacagattaa
     2881 taccccccgg gccaacattc ttacaggacc aactcatccg gctgtgtttc aacatgtttg
     2941 agcgaatcct tgtcccgacc gacttctcct ggtatgccca gcgaaccgtc cggtatgcaa
     3001 ccgagattcc gggagcgact ggtgttatac tcctgcacat cgccgaagat gcaggagagt
     3061 gcatgacgtg gccctcactc atggaagcga ggaatgcacc gtgcacaccc gccaatgcac
     3121 ttgcctatgc cggagatgcg ctcgagaagg ccgggttgtc cgtgacctac gaagtggcgc
     3181 ccccaaagga tgcaggcacc catgccgcta tcctcgcgtg tgcggagcag cacaaggcag
     3241 acctcattct gatcggtggg aaaggtaggg gtttttttaa ggacatgctc ctgggaagtg
     3301 tctccaggag ggtaatcgag gaagcaaaga cccacgtcct cgtcacccat ttcctggagc
     3361 agcccggtca cgggaatgag gccccggatg tgcctgttgc cacgggaaga ccgcttttct
     3421 cgcggatcct tgtccccgtg gacttctcaa ggccaactta cgagaccatc gaattcctga
     3481 gcgacgtggg cgggtatgac gagatcatcc tcatgcacgt catcgcctcg gtcgaggacc
     3541 ggcatgacct gcagaagaag ctccagcact ccatgcaggc gctctcatat ctacaacggg
     3601 accttgcgct cggaggcggg aagatcatcc ccctcatccg tttcggggat accgtcgggg
     3661 agatctgcag gatggcagac gaggagcgcg ctaccctgat cctcatctcc agattcggtg
     3721 catcagacta catgaaggat ggatccatcg gcagcatcgc agaagaggtg acaaagaggg
     3781 cccgcgttcc tgtcctcgtc cgttacccag gctcgtccct cgatgtgcag gtccgggagg
     3841 tcggcgctga tgaatttcca cgtgcagggg aggtatgggc ccattaccgg cagcagcatg
     3901 cagatccctc caccgatcgc atattcggcg tatttgtcca gggggatcct gtctccctcg
     3961 cccgatgcag gcggttccct gacggcctgg aagtggacgg cgtgtttacc ctggaagggt
     4021 tccgccgcag gggctatgca aggggcgcag tctcgctcct ggtgcgcgag tgcggaaccg
     4081 agcccctcta taccctctcc actgctgata tgatcgagtt cttcaggtct tttgggttta
     4141 atgagatccc ggaaaaggag atgcccggct ccatccggga acgctacagt tttgccctgg
     4201 gggacctggc aggcgcggac ctgtacccca tgagcaggag tgccaagggg gcgtcagaag
     4261 cccctgcggg ataggatggg aaagttgcgc gttctggatg gaaaatctcg actcgaacca
     4321 tccccgtgaa ggagaacggg gagcgctctg accggagt
//
LOCUS       DAOQYR010000141         4348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_20617_length_4348_cov_8.509434, whole genome shotgun sequence.
ACCESSION   DAOQYR010000141 DAOQYR010000000
VERSION     DAOQYR010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20617_length_4348_cov_8.509434"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    68..167
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            666..1940
                     /gene="thiC"
                     /locus_tag="PKM87_11340"
     CDS             666..1940
                     /gene="thiC"
                     /locus_tag="PKM87_11340"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNQ30704.1"
                     /translation="MSLIDDARAGIITDEMKIVARQEGVTEEFVRRGIAGGHIVIPIS
                     PYRDVKICGIGEGLRTKVNASVGTSSDIVDIDMEVEKTRQAELAGADTLMELSTGGDF
                     VEIRKRVISATSLSVGSVPLYQAFIEAARKDGAVVHMKEDDLFRITAEQAKLGTNFMA
                     IHTGINWETVKRLQNQGRHGGLVSRGGAFMTAWMLHNEKENPLYSEFDYLLEIMKEHE
                     VTLSTGNGMRAGAVHDATDRAQIQELIINTELGDKAHAEGVQVIIEGPGHIPVDEIQA
                     NVILQKRMSNRKPFYMLGPLVTDIAPGYDDRVAAIGAALSSSYGADFICYVTPAEHLA
                     LPTPDEVYEGVMSSRIAAHVGDMIKLKKRDADLEMGHARRDLDWERQFHIAMNPERAK
                     KIRDERMPADADACTMCGDYCALKIVNRHFSF"
     gene            2107..2505
                     /locus_tag="PKM87_11345"
     CDS             2107..2505
                     /locus_tag="PKM87_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNQ30705.1"
                     /translation="MKPESATILEEFLRHSDTMTSEIEQDAEEMLGVVPFIFRTLKER
                     PDLFVLSALGDYLACRPESLDAKTAELVAMTAAAVANAPDCLKVHIAAARKEGASHEQ
                     IRDCLIIASVIGKTTVLARSLRILEEQQGP"
     gene            complement(3020..3361)
                     /locus_tag="PKM87_11350"
     CDS             complement(3020..3361)
                     /locus_tag="PKM87_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNQ30706.1"
                     /translation="MTQTPEDALKVIQSHPEGVLQSELWKILEVDSRKCSRLVKKLLD
                     EGLIERVEFRKEGIKTFVLKASRRPVDPFLILAGEELIPCIGCDLECHIEECPRLLDW
                     MYQLAICEVDQ"
     gene            complement(3375..3611)
                     /locus_tag="PKM87_11355"
     CDS             complement(3375..3611)
                     /locus_tag="PKM87_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoprotein"
                     /protein_id="HNQ30707.1"
                     /translation="MVNGIVLPIKKVFSLVDSKVTVEIKDDQRKLQGRLVAVDEYLNI
                     HMEDTTEFVNNQRGRTLGTVVIRGNNILSIQPNI"
     gene            complement(3689..>4348)
                     /locus_tag="PKM87_11360"
     CDS             complement(3689..>4348)
                     /locus_tag="PKM87_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose 1,6-bisphosphatase"
                     /protein_id="HNQ30708.1"
                     /translation="ADPFNTAGLVIDPQMHDGFIFEVHDVIEKRKIEFRTPEESYSLL
                     AYIGAPSRYVIKYVKRKDGLIAASTSTQRLSLIAGKYVGKDDPVMIVRGQSGLPAVGE
                     IIDPFSIPIIVAGWMRGSHHGPFMPVGLPDANCTRFDGPPRVICLGFQVCNGKLIGPA
                     DMFDDPAYDRVRSRCNELADILRAHGPFEPHRLSLSEMEYTTLPAVEKALKGRWVPIK
                     E"
BASE COUNT          902 a         1236 c         1237 g          873 t
ORIGIN      
        1 atcaccgtcc tcttatattg cgcgcggaag tttcttcaag ctatggaagg agcagcagtg
       61 gctttatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggc cactgctgcg
      181 gggggtatgc gggggtggtc cggagggggg aaccccctcc gttcattgaa accagtacac
      241 ttttccactg cgttgattca gaccattcgt atcacacaat gacagagcag cagtggcttt
      301 atggccactg ctgcgggggg tatgcggggg tggtccggag gggggaaccc cctccgtcaa
      361 tgggaactct tatactctca ccgcgggtcg aacccgacta ttcctatccc tcaatatcac
      421 agcagcagtg ccaaaaggca ctcctgcggt gggtatgcgc gatgcctccc ctcatctttc
      481 ccggcttgaa cactccgggc tcccccatcc tctcatacct tcgcccatgc tcttccgctt
      541 gccgtccgat cttcaaatga agttgtctct acgcaaatca actttacttt agaagataac
      601 aagcgtaatt tagtcctatt tttgtaaaag aaccatttat cttgtctttc ctcccaccag
      661 tatgcatgag cttaattgac gacgccaggg ccggcatcat caccgatgag atgaagatcg
      721 tggcccggca ggaaggggtg accgaggagt tcgtccggag gggaattgcc ggtggtcata
      781 tcgttatccc gatctcccct tacagggatg tgaagatctg cgggatcggg gagggactgc
      841 ggaccaaggt gaacgcctct gtcgggacct cttcggatat cgttgacatc gacatggagg
      901 tggagaagac ccgccaggca gagctcgccg gggccgacac cctgatggag ctctccaccg
      961 gaggggactt cgtcgagatc aggaagcggg taatctcagc gaccagcctc tcggtcggaa
     1021 gcgtcccgct ctaccaggca ttcatcgagg ccgcgaggaa ggacggcgcg gtcgtgcaca
     1081 tgaaggagga cgacctcttc cgcatcacgg cggagcaggc gaagcttggg accaacttca
     1141 tggcgattca taccggaata aactgggaga ccgtcaagcg cctccagaac cagggccgcc
     1201 acggcgggct ggtctcccgc ggcggggcct tcatgaccgc atggatgctc cacaacgaga
     1261 aggagaaccc cctttactcg gagttcgact accttctgga gatcatgaag gagcacgagg
     1321 tcaccctctc cacgggcaat gggatgcggg ccggtgcggt acatgatgcc actgaccgtg
     1381 cccaaatcca ggaactcatc atcaacacgg aactcgggga caaggcacat gccgagggtg
     1441 tgcaggtgat aatcgagggg ccggggcaca tccctgtcga tgagatacag gcaaacgtga
     1501 tcctgcaaaa acggatgagc aaccggaaac ccttctacat gctcgggccg ctcgtgaccg
     1561 acatcgcacc gggatacgac gaccgggtcg ccgcgatcgg tgccgccctc tcctcatcgt
     1621 acggcgccga tttcatctgc tacgtcactc ctgcagagca cctggcgctc cctacccccg
     1681 acgaggtcta cgagggggtc atgagctccc gcatcgcggc ccatgtcggc gacatgatca
     1741 agctgaagaa gcgggacgcg gacctggaga tgggacatgc ccgtcgcgac ctcgactggg
     1801 agcggcagtt ccatatcgcc atgaaccctg aaagagcaaa gaagatccgg gacgagagga
     1861 tgcccgccga tgccgatgcc tgcaccatgt gcggcgacta ctgtgccctg aagatcgtga
     1921 accggcactt cagtttctaa atcctttttt ccagccgttt cgtctgagtc cgctcccgca
     1981 gtgaatgagc cagggtctcc gagtgttacc atgatctgaa ttgcgaactt gtgcgcaccc
     2041 gatattcccc atccccccac aatggatttc cataccttca tctctcctcc actcgaacca
     2101 ccagcaatga aaccggaatc tgcgacaatc ctggaggagt tcctcaggca ttccgatacc
     2161 atgaccagcg agatcgagca ggacgcagaa gagatgctcg gcgttgtacc gtttatcttt
     2221 cgcaccctca aagaacgccc cgatcttttc gtcctctcgg cacttggcga ctaccttgcc
     2281 tgccgccccg agagcctcga tgcaaaaacg gcagaattgg ttgccatgac agctgctgct
     2341 gtagccaatg caccggactg cctcaaggtg catatcgctg cggcacgaaa ggaaggagca
     2401 tcccatgagc agatcaggga ctgcctgatc attgcatcag tgatcgggaa gacaacagtg
     2461 ctggcgcgat cgttgcggat attagaggag cagcaaggac cgtaaggtca ttgctgcggg
     2521 gggtgtgcgg gggaggcgcc ggagggggga accccctccg gtatagaccc tggtatacta
     2581 ttagcctggg ggtatgcaga tcgcgacccc ggtgcactct ataccttttc tgaaaccgac
     2641 cgcaatccac atacgattac actcacaaat ctgatgctcc gcctagaatg cgataagatc
     2701 ggtgcttgag agtagggagg gatgagaaga gccccgatgc acccatgccc acggactatc
     2761 gcatatcggg ggtagggaca ggggttgggg attctccccc tcccgtgata ataaaaacgc
     2821 acttcctccc attcccgcag gtttaaagcc agtgccgggg tgcagatgaa aatccgacgt
     2881 aatcctcgtc tcgccgaggc gtcgaggggc gccaaccctc gcggcatttc ccctcatacc
     2941 atatgcttca ggaagttgcg aattctccct ttattttcgt gctattaaaa taaagaccag
     3001 agagaaaaga gcgagacccc tactggtcca cttcgcagat ggccagctgg tacatccagt
     3061 cgaggaggcg tgggcactcc tcgatgtggc actccaggtc gcagccgata caggggatca
     3121 gctcctcccc tgcaaggatg aggaaggggt ccacgggccg gcggctcgcc ttcaggacaa
     3181 aggtcttgat accctccttc cggaattcta cccgctcgat caatccctcg tcaaggagct
     3241 tcttgacaag gcgggaacac ttgcggctgt ctacctcgag gatcttccag agctcgctct
     3301 ggaggactcc ttctggatgc gactggatca ccttgagtgc atcttcgggg gtctgcgtca
     3361 taggccagga acttttagat attgggctgt atggagagga tattgttgcc ccggatgacc
     3421 acggtcccca gggtgcggcc cctctggttg ttgacaaact cggtggtgtc ttccatgtgg
     3481 atattcaggt actcgtccac cgcgacgagg cggccctgga gctttcgctg atcgtccttg
     3541 atctccaccg tgaccttgga atccaccaga gaaaagacct tttttatggg taatacgata
     3601 ccgttcacca tctgcctctt attggggggt gccggtattt attgcttttc cgccggaacc
     3661 tggcaggtcc atataaaaaa atgcggggtt attccttgat tgggacccag cggcccttga
     3721 gcgccttctc gactgccggg agcgtggtgt actccatctc cgagagagag agccggtgcg
     3781 gctcgaaggg cccgtgggcc cggaggatat cggcaagctc gttgcaccgg gacctgaccc
     3841 ggtcgtaggc agggtcgtca aacatgtctg ccgggccgat cagctttccg ttgcagacct
     3901 ggaacccaag gcagatcacg cggggcgggc cgtcgaagcg ggtgcagttc gcatcgggga
     3961 gcccgaccgg catgaagggg ccgtggtgcg agccccgcat ccacccggcg acgatgatgg
     4021 gaatgctgaa cgggtcgatg atctctccga ccgcaggcag tccgctctgg ccccgcacga
     4081 tcatgacagg gtcatccttc ccgacgtact tcccggcaat gagggagagc cgctgggtgc
     4141 tggtggacgc ggcgataagg ccgtcctttc gcttcacgta cttgatcacg taccgggaag
     4201 gggcgccaat ataggccagc aggctgtagc tctcctcagg tgtgcggaac tcgatcttcc
     4261 gcttctcgat cacgtcgtgg acctcgaaga tgaacccgtc gtgcatctgc gggtcgatga
     4321 cgagacccgc agtgttgaac gggtcggc
//
LOCUS       DAOQYR010000142         4260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_21159_length_4260_cov_8.428537, whole genome shotgun sequence.
ACCESSION   DAOQYR010000142 DAOQYR010000000
VERSION     DAOQYR010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21159_length_4260_cov_8.428537"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..879)
                     /locus_tag="PKM87_11365"
     CDS             complement(112..879)
                     /locus_tag="PKM87_11365"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ30709.1"
                     /translation="MAKADKPEQPASSRQKYRRWILLLTFIAIPVTIFYISPIVIMMG
                     ASEGIVTGSFLLYIVLFVLSLFAGRIWCGWLCPMGACQELGSRLIKKNVKEGWPNWIK
                     YGVTLLWIAAIANAFFAAGGVQGVDPFYGTINGISVSSLQVLMMVVMIFTGIFVVAFL
                     TGKRGFCHILCPIAGVMVLGRKIRNLAGWTALEIDADSGRCTDCLTCSEKCPAGLDVH
                     GMVRKGTMENPDCIMCATCADVCPERVIRYGVGGRMK"
     gene            1078..1905
                     /locus_tag="PKM87_11370"
     CDS             1078..1905
                     /locus_tag="PKM87_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNQ30710.1"
                     /translation="MHTLRSLLLFAILLISLLGSGCLDGLETKAPDHNSMLVILGDAT
                     GAIQGALDELERVASMTAASLEKTGIAGPDAEAVLKGRISSYPPVLTMITCNANGTVV
                     AAEPESVKVLVGQDLSGQEVVQRTLAEKVPVMSGMFPLAEGGNGVAVAHPVRSADGKF
                     LGVISTMFVPYDLIRPIAEDAANGTPYTFMVIETGGHILYDPDPGEVGKETFGDALYA
                     QFPEIDRVARHAAANRSGYDTYSFYGTGSDAVVRKETFWSTVSLHGTEWRVMVIGEG"
     gene            2323..3165
                     /locus_tag="PKM87_11375"
     CDS             2323..3165
                     /locus_tag="PKM87_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30711.1"
                     /translation="MGLLGGLLDYYRGLGLESHIAQALFILGIFAVGGIIFIFFFALH
                     VPYEMSLVNMTVANATNATIPVETGTKILSSWDSAFSVISVFGVGVLVAGGSFFLGGL
                     LGFLFGIPKMISQPAGGDNTARTSQQYRANTNLEDISDWLTKIVIGVGLVELSAVPSL
                     LDMYAKMIAPALGGVPSSGAVAIAIFLYYSLVGFLIVYLATRGYMESDLEKQERMELY
                     REKVEKLEKELQLDQRKLEAESNLIKMNNLAYEKDILELTRRIQEMKLQTNSQETADT
                     ANEE"
     gene            3414..>4260
                     /locus_tag="PKM87_11380"
     CDS             3414..>4260
                     /locus_tag="PKM87_11380"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNQ30712.1"
                     /translation="MIRRHMVLVAFFFAVAVLSAGCTDTTGTKTGTPVGTPIPAPTTT
                     IAAHTPPDKITTTPELAAFVAHAAAFARGNGRDATIAAFNDPDGPFVIGTVQVFALDY
                     SGNLLADAGEPEVVGTNIQDMADSFGIPLVRKLAETAGFRRGYVSYTYPNPAKNNTFE
                     PRIAVVEDVDGTYYVGAAMFASEGEVFPSVKLNTSAAHPDVDDLVAYVKNAVAYAKEH
                     GNEKALSEFNNPDGQFVQGELVMMAFDYDGTNLAAPPYSPELVKYRINLINYQDPDGV
                     ETIRGM"
BASE COUNT          981 a         1176 c         1208 g          895 t
ORIGIN      
        1 cgacatggta ttgtgaacgt cgggggggat ggtatttata ggttggtgga gagggccggg
       61 taagaaaata tgagagaatt ttaaccctgg ggcaggagat ccccctctct cttatttcat
      121 ccttcccccc acgccgtacc ggataaccct ctccggacac acatccgcac acgtcgcaca
      181 catgatacag tctggattct ccattgtacc tttcctgacc atgccatgca cgtcgagacc
      241 tgccgggcat ttttccgagc aggtgaggca gtcggtgcac ctgccggaat cggcatcgat
      301 ctcgagtgcc gtccagccgg cgaggttccg gatcttccgg ccgagaacca tgacgccagc
      361 gatcgggcag aggatatggc agaaaccgcg tttgcccgtg agaaacgcga caacaaagat
      421 tcccgtaaag atcatcacaa ccatcatgag gacctggagg gacgagaccg agatcccgtt
      481 gatcgtgcca tagaaaggat cgacgccctg gactccgcct gcagcgaaaa acgcgtttgc
      541 gatggcagca atccagagca acgtgacgcc gtatttgatc cagttgggcc atccctcctt
      601 cacgttcttc ttaatcagcc gggacccgag ctcctggcag gcacccatcg ggcagagcca
      661 cccgcaccag atccgccctg cgaaaagaga gaggacaaag agcacgatgt agagcaggaa
      721 ggagcccgtc acaataccct cggatgctcc catcatgatg acaatcgggg agatgtagaa
      781 tatcgtcacc gggattgcga tgaaagtgag caaaaggatc caccgccggt atttctggcg
      841 ggatgatgca ggctgttcgg gcttatctgc tttggccata ctttattcct gcatttcagg
      901 tatgattcgg atgacaataa ttcaggcggt ctccctgtgc cgggcgatga gttcagaggg
      961 aagcggggga ttctgatccc ctgatacccc gaacctctcc ttcatgttac tggtaaaaag
     1021 tacaaccctt ctgatcgcaa accacaagcc accctgccac cgatacatac ttcacacatg
     1081 cacaccctcc gttccctcct actcttcgcc atcctgctca tctcacttct tggttctggc
     1141 tgcctggatg gactggagac gaaggccccc gatcacaata gcatgctcgt aattctagga
     1201 gatgcaaccg gcgccatcca gggtgccctt gacgaacttg agagagtcgc ctcgatgaca
     1261 gccgcgtcgc tcgaaaagac cgggatcgcc gggcctgatg cggaggccgt gctgaagggg
     1321 aggatctcat cctacccccc cgtcctcact atgatcacct gcaatgcgaa cggcacggtc
     1381 gtcgccgcgg agccggagag cgtgaaggtg cttgtagggc aggacctctc cggccaggaa
     1441 gtcgtgcagc ggacgctcgc ggaaaaggtc ccggtgatga gcggcatgtt cccgcttgcc
     1501 gagggaggaa acggggtggc agtcgcgcac cctgtcaggt cggcggacgg aaagttcctt
     1561 ggggtgatca gcacaatgtt cgtgccgtac gatctgatca ggccaatcgc ggaggatgcg
     1621 gcgaacggga ctccgtacac cttcatggtg atcgagacag ggggccatat cctctacgac
     1681 ccggacccgg gagaagtggg gaaagagacc ttcggggatg cgctctacgc acagttcccg
     1741 gagatcgacc gggtcgcacg ccacgctgcc gcgaaccgct cgggatacga cacctactcg
     1801 ttctacggca cagggtcgga tgcggtcgtc cggaaagaga ccttctggtc gacggtcagt
     1861 ctccacggga cagagtggag ggtgatggtg atcggggagg ggtgaggagg attctccccc
     1921 attcttggtg ctacagcaat tcctatcctg cgccgaaaca gggggaggtt acgggatatt
     1981 ccctccgccc actatactgc taccttgccc acctccataa tgatttatgc ggacgatggg
     2041 actctctatc cagttatact cacgaaatac catacctagc ggacctttta ccaggtgcga
     2101 cagagggaac cgcctccaca gggtatactg tatttcctga aaataccgta tctttcctgg
     2161 accgcttcga atcgcttttt gattggggtg cattgacgca ccccatgcaa cacgggtaaa
     2221 cggaggggat ccagggaagg ccggatcctt ctgggggtca agccttatat acccaaacca
     2281 gttattcttc attacgaata aaaaaatcag cggggggact tgatggggtt gcttggcgga
     2341 ttgctggatt actaccgtgg cctgggcctt gagagtcata tcgcacaggc actcttcatt
     2401 ctcggtatct ttgcggtagg gggcattata ttcatattct tcttcgcact ccatgtcccc
     2461 tacgagatgt ctcttgtgaa tatgactgtc gcgaatgcaa cgaacgccac catcccggtg
     2521 gaaacgggca cgaaaatcct ttcgtcctgg gactcggcat tctccgtaat ctctgtcttt
     2581 ggggtagggg tactggttgc aggcggttcc ttcttcctgg gcggtctgct tggtttcctg
     2641 tttggaatcc cgaagatgat atcgcaaccc gcgggaggag acaataccgc aaggaccagc
     2701 cagcaatacc gggcaaacac gaacctcgag gacatttcag actggttgac caagattgtg
     2761 attggcgtgg gactggtcga actgagtgcg gtcccttcac tcctggacat gtatgcgaag
     2821 atgattgcac cggctttagg gggagtcccg agcagcggcg ctgttgcgat cgcgattttc
     2881 ctgtattatt ctctggtggg tttcctcatc gtgtacctgg cgacaagggg gtatatggag
     2941 agcgatttag aaaagcagga gaggatggag ttatacagag agaaagtcga gaaactggag
     3001 aaggaactgc agcttgacca gaggaaactg gaggcagaat ctaacctgat aaagatgaac
     3061 aaccttgcct acgaaaagga catattggag cttaccagga gaatacaaga gatgaaactt
     3121 cagaccaatt cccaagaaac cgcggatacc gccaacgagg aataagtctg gtacatcctt
     3181 tcccttgaga acttcgtccc tatgcgaggg aggatggcgg aattggtatg aagggccgtc
     3241 cggaaagaga ccttcttttt gactgtcgtc ttcactggac ggagtagatg atgatagggg
     3301 agagggggta ttgctatcaa aaggaaacgg ggttcctttg aatggtttca tgccccccct
     3361 aactctccac caattccaca acccttttgc ccccctgcac gaaccggtag ttcatgattc
     3421 gcagacatat ggtcctcgtt gccttcttct tcgcagtggc ggttctttcc gccgggtgca
     3481 ccgataccac aggaacaaag accgggaccc cggtaggaac accgattccc gcaccgacaa
     3541 ccacgatcgc agcccacacc ccacctgaca agattaccac cactccggaa cttgccgcgt
     3601 tcgtcgcaca cgccgcggca tttgcacgtg ggaacggaag ggatgcgacg attgccgcgt
     3661 tcaacgatcc ggacgggccg ttcgtcatcg gcaccgtcca ggtgttcgcc ctcgattata
     3721 gcggaaacct ccttgcggat gcgggcgagc ccgaagttgt cgggacgaac atccaggaca
     3781 tggccgactc ctttgggata ccgctcgtgc gaaagctcgc agagaccgcc gggtttagga
     3841 ggggatatgt gagttacacc tacccgaacc cggcaaagaa caacacgttt gagccgcgga
     3901 tcgccgttgt cgaggacgtg gatggcacct attacgtggg tgcggcgatg tttgcatccg
     3961 agggagaggt cttcccgtcg gtgaaactga atacgtccgc agcgcaccct gatgtcgatg
     4021 acctcgtcgc ttacgtgaaa aacgccgtcg catacgcgaa ggaacacggg aatgagaagg
     4081 ccctctcgga attcaacaac cctgatgggc agttcgtgca gggcgagctt gtcatgatgg
     4141 cgttcgatta cgacggcacg aacctcgcgg ccccgcccta ttcccccgag cttgtgaaat
     4201 accgaatcaa cctgatcaac taccaggacc ctgacggcgt agagaccatc cgcgggatgc
//
LOCUS       DAOQYR010000143         4129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_22068_length_4129_cov_9.251841, whole genome shotgun sequence.
ACCESSION   DAOQYR010000143 DAOQYR010000000
VERSION     DAOQYR010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22068_length_4129_cov_9.251841"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..932)
                     /locus_tag="PKM87_11385"
     CDS             complement(15..932)
                     /locus_tag="PKM87_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNQ30713.1"
                     /translation="MSQERLLSTVTSVLLMAGFRVSEPFSMRPRSFDLIARHREMILV
                     IKVVSHIDSVSEEVARDLDHIARYLRGAPLIIGDRARDADLERGAVYVRYGIFAISVA
                     TLYDAFVEGTPPLVYASPGGLYVSINGEVLRELREKMNLSLGDMGTLLGVSRRTISKY
                     ESGMGTTLEVAQKIEEIFDAPLVRSIDLLRYSSLFEDEPEKEEEPAPMGFLQRIGVKL
                     HAMHRAPFQALIEISDQSILTGYGSSQKVVKRAALIGNISQVAGMHAMCVLTDYAKQK
                     KIGKTLVIGEQRLLALEDGEELIQLVSKS"
     gene            982..2229
                     /locus_tag="PKM87_11390"
     CDS             982..2229
                     /locus_tag="PKM87_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile)(2)-agmatinylcytidine synthase"
                     /protein_id="HNQ30714.1"
                     /translation="MRIGIDDTDSPRGMCTTYLGAVLGKRLARAGIAVREARLVRLNP
                     NVPWKTRGNAAVCLVAEGSPKKAFRLACNTVGELSDLGHEHTHPGVVVSGDDLPVEFY
                     RKAVQGFCTLDEAQAVLDEAGALSRGYKQKRGLIGATAAACSNFDDATYELLAYRDPA
                     SWGTPRFVERESLFSADLHTYPHTWDTVDRENDLVVCVPHTPDPVLFGIRGERPGWVA
                     LAREEVVSERPSLEQVWITNQGTDAHLVPAISPEFEEGCSYLLAGAVGSLPVTHRGGH
                     VSFTLASGYRCLTCMAYEPTRGFRGVVRGLLPGDRVRVAGSYKGGSINLEKLQVVSAP
                     PLRIARPPVCGRCGKRMTSAGTDKGYKCRRCGGRERAPEILEESRVIAPGWYEVPPSA
                     RRHLARPLCRGLRQALLSLVPCP"
     gene            2274..3203
                     /locus_tag="PKM87_11395"
     CDS             2274..3203
                     /locus_tag="PKM87_11395"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HNQ30715.1"
                     /translation="MQCGDPVEQVSIRAGMTVGELVSALGRAGAYNGGALAKAVSVYE
                     AMLRDDQAVKFMGLAGAMVPAGMGRIVSDLIDRGHVDVLVSTGANLTHDIIEALGCRH
                     YHGSAACSDIELREEEINRIYDVFLPNDAFIRFEEFMQEVFSGLSPVAPMKICELLAV
                     IGRALPSGILASTSRKGVPVYCPALADSMIGLQYWLFSQTHRVTVDAFLDLQPLLDTC
                     FGAERAGALLIGGGVPKNFILQSMLMTPRGFDYAVQLTGDRPDLGGLSGATLDEARSW
                     GKITGSASAVTVYGDATITLPLLVAAVLERVEK"
     gene            3200..3835
                     /gene="pyrF"
                     /locus_tag="PKM87_11400"
     CDS             3200..3835
                     /gene="pyrF"
                     /locus_tag="PKM87_11400"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNQ30716.1"
                     /translation="MTELILALDVVDRDRALAVARACAPQIDAVKIGYPLVLSAGLSI
                     ARGLSDLGLPLIADFKVADIPHTNRLIAEQVFSAGFGALICQGFAGRDSVTACVDAAR
                     AMGGRCMVVAEMSHPGAVEYFSGGVAERIARMAAECGADGIIAPATRPDRVRVLREIV
                     RERLIWSPGVGAQGGDPRAIAGLVDAVIVGRQIYGAEDPAREAAAFEFLRR"
     gene            3945..>4129
                     /locus_tag="PKM87_11405"
     CDS             3945..>4129
                     /locus_tag="PKM87_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNQ30717.1"
                     /translation="MQWSKEQLEMARSVRRLEDIPVPERKYKCHTCHLIVDETPCPNC
                     GDTHLQIMCPLDHCHCS"
BASE COUNT          760 a         1286 c         1302 g          781 t
ORIGIN      
        1 gttctatata aaaattatga tttactgacg agctggatca gctcttcccc gtcttccagg
       61 gccaaaaggc gctgttcccc gatcaccagg gtttttccga tcttcttctg tttggcgtaa
      121 tccgtgagta cgcacatcgc atgcatcccg gcgacctggg agatgtttcc gatgagcgca
      181 gcgcgcttca ccaccttctg ggatgacccg tacccggtga ggatggactg gtcggatatc
      241 tcgattaagg cctggaacgg ggcccggtgc atggcatgca gcttcacccc tatgcgctgc
      301 aggaatccca tcggtgcagg ctcttcctcc ttttcgggct cgtcctcgaa gagcgacgag
      361 taccggagga ggtcgatgct tctcacaagg ggggcatcaa agatctcctc gatcttctgc
      421 gccacctcga gcgtcgtccc catcccgctc tcatacttgc tgatggtccg cctggagacc
      481 ccgagcaggg tccccatgtc tccaagcgag aggttcatct tctcccgcag ctccctgagc
      541 acctcaccgt tgatgctcac gtagaggccg cccggcgatg catacacgag gggtggtgtc
      601 ccttccacga atgcgtcgta aagggtggcc acgctgatgg caaagatgcc gtacctcacg
      661 tacacggccc cacgctccag gtcggcgtcg cgtgcccggt cgccgatgat cagcggcgct
      721 cccctgaggt accgggcgat atggtcgagg tcccgtgcaa cctcctcgct cacgctgtcg
      781 atgtgagaga ccaccttgat cacgaggatc atctcccggt gcctggcgat caggtcgaaa
      841 ctccttggcc tcatgctgaa cggttccgag acccggaacc ctgccatcag caagacactc
      901 gtcaccgtgg agagaaggcg ttcctgtgac atcccactag gaatggattt agactgccct
      961 gatataaata ataggctgaa catgcgcatc gggatcgacg acaccgattc tccccggggg
     1021 atgtgcacca cctaccttgg ggcggtgctt ggaaagaggc tggcacgtgc cggcatagcg
     1081 gtcagggagg caaggctggt gcgcctgaac ccgaatgtcc cctggaagac aagggggaat
     1141 gcagccgtgt gcctcgtcgc cgaagggtcg ccaaaaaagg cattccgtct tgcctgcaac
     1201 accgttgggg agctttcgga ccttgggcac gagcacaccc atcccggggt ggtggtctcc
     1261 ggcgacgacc tgcccgtgga gttctaccgg aaagcggtgc aggggttctg cacccttgat
     1321 gaggcccagg cagtcctcga cgaggcaggg gcgctttcca ggggttacaa gcaaaagcgt
     1381 ggactgatcg gagcgaccgc cgcggcctgc agcaatttcg atgacgccac gtacgagctg
     1441 ctggcgtatc gcgatcccgc gagctggggc actccgcgtt ttgtcgagag agagagcctc
     1501 ttttcggcag acctccatac gtatccccat acctgggaca ctgtcgatcg tgagaacgat
     1561 ctcgtggtct gcgtcccgca cacccctgac ccggtactct tcggaatacg gggggagcgg
     1621 ccagggtggg tggcgctcgc ccgggaggag gtcgtctccg agagaccttc actcgagcag
     1681 gtctggatta ccaaccaggg gaccgatgcc cacctcgtcc ccgccatctc ccccgaattc
     1741 gaagagggct gctcgtatct cctggccggg gcagtcgggt ccctgcccgt gacccaccgg
     1801 ggcgggcacg tctccttcac gctggcctcc gggtaccgct gcctcacctg catggcgtac
     1861 gagcccacca gaggcttccg tggcgtggtg agggggcttc tccccgggga ccgggtgagg
     1921 gtcgcgggga gttataaagg cgggagcatc aacctcgaga aactccaggt cgtctctgcc
     1981 cccccgctgc ggatcgccag gcccccggta tgcgggcgtt gcggaaagcg gatgaccagt
     2041 gcggggacag acaagggtta caagtgccgg aggtgcgggg ggagagagag agcgcccgag
     2101 atcctcgagg aatcgagagt catcgcaccg ggatggtacg aggtgccccc aagcgcccgc
     2161 cgtcacctgg cccggcccct ctgcaggggg ttgcgacaag ccctcctctc cctggtgccc
     2221 tgcccctgaa ccagacgaag gaatattctc ttcactaccc catatcctgt accatgcaat
     2281 gcggggatcc ggtcgagcag gtctcgatcc gcgcggggat gactgtcggg gagcttgtca
     2341 gcgccctcgg gcgggcgggg gcctacaacg ggggtgcgct ggccaaggcg gtttcagtct
     2401 acgaggccat gctaagggac gaccaggcag taaagttcat gggccttgca ggggcgatgg
     2461 ttcctgccgg gatgggaagg atcgtaagcg atctcatcga tagaggccac gtcgacgtcc
     2521 tggtctccac cggcgcgaac ctcactcacg acatcatcga ggcgctggga tgcaggcatt
     2581 accatggatc tgccgcatgc tccgacattg agctccgtga ggaggagatt aaccggatct
     2641 acgacgtgtt cctcccgaac gatgcgttca tacggttcga ggagttcatg caggaggtct
     2701 tctcgggtct ctcacctgtc gccccgatga agatatgcga actcctcgcc gtcatcggcc
     2761 gcgcccttcc gtctggaatc ctcgcctcca cttcaaggaa gggggtgccg gtatactgtc
     2821 cggctctcgc ggactcgatg ataggtctcc agtactggct gttctcccag acccaccgcg
     2881 tcaccgtgga cgcgttcctc gacctccagc cgctcctcga cacgtgcttt ggggcagaga
     2941 gggccggcgc cctcctcatc ggggggggag tccccaagaa cttcatcctc cagagcatgc
     3001 tgatgacacc ccgggggttc gactacgcgg tgcagctgac cggggaccgc cccgacctcg
     3061 gcgggctctc tggggccacc ctcgacgagg cgcgttcgtg gggaaagatc accgggtcgg
     3121 cgagtgccgt gacagtctac ggggatgcga ccatcacgct cccccttctt gtcgcggcag
     3181 ttcttgagag ggtggagaaa tgacggaact gatactcgca ctcgacgtgg tggaccggga
     3241 ccgtgcactc gctgtcgcaa gggcatgcgc ccctcaaatc gacgcggtca agatcgggta
     3301 tcccctcgtt ctctcggcag ggctgtccat cgcacgcggg ttatcggacc tcggccttcc
     3361 cctcatcgcc gacttcaagg tggccgatat cccccacacc aaccgtctga tcgccgagca
     3421 ggtcttctct gccgggttcg gcgcattgat ctgccagggc tttgcgggca gggactcggt
     3481 cactgcctgt gtcgacgcag cccgggccat ggggggccgg tgcatggtgg tcgcagagat
     3541 gagccacccg ggggcggtcg agtatttctc aggaggagtt gccgagcgga tcgcccgaat
     3601 ggcagcggaa tgcggcgccg acgggatcat cgcccccgcc acacgccctg accgtgtgcg
     3661 ggtgctccgg gagatcgtca gggaacggct gatctggtcc ccgggggtag gcgcacaggg
     3721 cggagacccc cgcgccattg cagggctcgt ggacgcagtg atcgtgggga ggcagatcta
     3781 cggcgcagaa gacccggcaa gggaagcggc ggccttcgag ttcctccgcc gatgatgatc
     3841 caactctctc tgagggaatt tcccggtgat gacccctatg agtggactga ggcgaaatct
     3901 ggcacaagct aaaggtttaa atggaaaaat atccatggta atgtatgcaa tggagtaaag
     3961 aacagcttga aatggccaga tccgtgcgcc gcctcgagga tatcccggtc ccggagcgga
     4021 agtacaagtg ccacacctgc cacctgatcg tggacgagac cccgtgcccg aactgcgggg
     4081 atacgcacct ccagatcatg tgtcccctgg accattgcca ctgttccca
//
LOCUS       DAOQYR010000144         4059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_22598_length_4059_cov_5.448052, whole genome shotgun sequence.
ACCESSION   DAOQYR010000144 DAOQYR010000000
VERSION     DAOQYR010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22598_length_4059_cov_5.448052"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PKM87_11410"
     CDS             complement(<1..405)
                     /locus_tag="PKM87_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNQ30718.1"
                     /translation="MNLFKKQKGTEVSPATPGTPVAQRTRTPGFVRDFDSIFDDFRRS
                     VDSLMRPYFPLDFPRDLSDWQLFQYAPLDLIDEGDHYKVYVELPGLSKEDVEVDLNNE
                     SLSIQGQRKEETEKREKNYLHRERYYSGFKRSI"
     gene            complement(605..1111)
                     /locus_tag="PKM87_11415"
     CDS             complement(605..1111)
                     /locus_tag="PKM87_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HNQ30719.1"
                     /translation="MFKDKEIVGVCEVNGPSYYGIHNIFRRLLENIQDIIVIAICVLL
                     FALMIKALVGMFYEFLEPFNFQYIASELIYILVLVEIYRLLIIYLREHRIAVDIMIEV
                     GIVSILREVILHGILGIDPFILIAIAILLISLMLLLRFGALRKEEVESENRGNIFSEL
                     KRSRAEKG"
     gene            complement(1098..1502)
                     /locus_tag="PKM87_11420"
     CDS             complement(1098..1502)
                     /locus_tag="PKM87_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNQ30720.1"
                     /translation="MRQIVVRQIERPMLRDEDEDLQWFCTSLGIGEGRDVERVATRVL
                     FTLLDHQPYEKGLPVDTLAQDLAVSSSRINHHIRNLVDAGAVYRHKRLIYLRGNSLRS
                     MVQEIRKDVLRVLDDLELAAAEIDQSFGIVQR"
     gene            1641..1916
                     /gene="groES"
                     /locus_tag="PKM87_11425"
     CDS             1641..1916
                     /gene="groES"
                     /locus_tag="PKM87_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNQ30721.1"
                     /translation="MKEIIPVGERVLIKPLKLEEMTRSGIYIPESAQEHRKEGSVIAV
                     GSRDDGSALPLKPGDHVLYGGYSADKFEVDGENFVFVPFKDILAKIE"
     gene            1928..3541
                     /gene="groL"
                     /locus_tag="PKM87_11430"
     CDS             1928..3541
                     /gene="groL"
                     /locus_tag="PKM87_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106636.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNQ30722.1"
                     /translation="MAISKQLMFDEEARKALLSGVNKVADTVKITLGPKGRYVVIDKA
                     MNPIVTNDGVTIAKEISLHDKFENIGAKLVKEVAQKTQDKTGDGTTTATLLAQAILTE
                     GLKNITSGANPIEIKRGIDTAIAAVVEYIGSTSVPVKGREGILQVATVSANNDEEIGK
                     LISDAMEKVGYGGLITVEDAKSLETSLDVVKGMQFDRGFISPYMVTDHEKMTCEYEDP
                     NILITDRKITSLKQMIPVLEMASQEGKPLLIIAEDVEGEALAALILNIIRGSFKVCAV
                     KAPGYGEERKAILEDIAILTGATVISEERGMKLENVSRAALGSAHTIKIDGEKTLIVE
                     GKGDRKALDDRMHLIESQINITDSEFKKEELKKRLGNLSGGVAVIKVGAATETELKEK
                     KMRIDDALNATKAAVEEGVVPGGGVTLFRAIESLDRLSFDDDRKIGMAIVRRALEEPL
                     RQIAKNAGVEGAEVIAMIKGREENGFGYNAKTGTYENLMEHGVIDPAKVVRIGLQNAG
                     SISGMILSTEVAITDFDEEKDKQSAAIII"
     gene            3841..3993
                     /locus_tag="PKM87_11435"
     CDS             3841..3993
                     /locus_tag="PKM87_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30723.1"
                     /translation="MTFNHKPDEQASLLKQIAEAEEQKKMSYFISVPGCFYEIEYGLV
                     KGWKRG"
BASE COUNT         1094 a          979 c         1065 g          921 t
ORIGIN      
        1 gatactcctt ttgaagccgg aataataccg ctcccggtga agataattct tctctctctt
       61 ctcggtctct tcctttctct ggccctgaat ggagagactc tcgttgttga ggtcgacttc
      121 aacatcctct ttggacagcc cggggagctc cacgtagacc ttgtagtgat ctccttcatc
      181 gatgagatcc aatggcgcat actggaacaa ctgccagtca gagagatcac ggggaaagtc
      241 cagcgggaag tacggccgca tgagactgtc gacagatctc ctgaagtcat cgaagatcga
      301 atcaaaatcc ctgacaaatc cgggtgtccg ggttcgctgt gcaacaggag tgcctggtgt
      361 tgcaggagac acttctgtac ctttttgctt tttaaagagg ttcataggtg cttcctccta
      421 tgttcacttg aagttagtct tattaaaata tcaatatttc tcatacggca atccggcccc
      481 cttgcatccc gggatctacc ttgcactcgt tggaaaaaaa tgcctgcgcc ccttcggata
      541 tacgagcaga aatttccatc ttgcaaacca gttcgtaata agattataga gggatttttc
      601 cctgtcaacc tttctcagcg cgagatcgtt tcaactcgct gaatatattc ccacgattct
      661 ctgactctac ctcctctttt cgtaatgcac cgaatcgcaa gagaagcatc agtgatatga
      721 gaaggattgc aatcgcgatg agtatgaagg gatcgatgcc caggataccg tggaggatga
      781 cttccctgag aatggagacg attccgacct caatcataat atccacggca atacgatgct
      841 cccggaggta gatgatcagg agccggtata tctcgaccag gacgaggata tagatcagct
      901 ccgaagcaat atactggaaa ttgaacggct ccaggaattc atagaacatc cccactaacg
      961 ccttgatcat gagcgcgaac agcaacacgc atatggcgat gacgatgata tcctggatgt
     1021 tctcaagcag gcgtcgaaag atgttatgga tgccatagta ggatggtccg ttcacctcgc
     1081 atacccccac tatctcctta tctttgaaca ataccaaacg attgatcaat ctctgctgct
     1141 gccagttcaa ggtcatcaag aacccggaga acatctttcc ggatctcctg gaccatggaa
     1201 cgcaggctgt ttccccgaag gtaaatcaac cgcttgtggc ggtataccgc cccggcgtct
     1261 acgagattcc tgatatgatg gttgatccgc gaactggaga cagcaagatc ctgtgcaagg
     1321 gtatccacgg gcaagccctt ttcatagggc tggtgatcga gcagggtgaa gagaaccctg
     1381 gtggcaaccc gctccacgtc tctaccctcg ccgataccga ggctggtaca gaaccactgg
     1441 agatcttcat cctcgtcccg gagcatcggc cgctcgattt gcctgaccac gatctgcctc
     1501 atggtaaccg atcaaaatct cgctacccat ccataaataa gtttgcaata atattcaaat
     1561 ggtatcgaca ttattgataa tttttaaata tttttgatat caaaagaatg gaatgcaata
     1621 atttccagag gtgcatatga gtgaaggaaa ttattcccgt cggagaaagg gttctgataa
     1681 aacccctgaa actggaggag atgacaagaa gtggcatcta cattccagag tccgcccagg
     1741 aacatcgaaa agaaggatcc gtcatcgcgg tgggaagtcg ggatgatggt tctgcactcc
     1801 ccctcaagcc gggagaccat gtattatacg gcggatatag tgcagataag ttcgaggtag
     1861 acggagaaaa tttcgttttt gtccccttca aggacattct ggcaaaaatt gaataagggg
     1921 gatggacatg gcgatatcca aacagctgat gttcgacgaa gaggcccgaa aagctcttct
     1981 ttctggtgtc aacaaagtag cggacacggt caagatcacg cttggcccga aagggcggta
     2041 tgtggtgatt gacaaggcca tgaaccccat cgtgacaaac gatggagtga cgatcgcaaa
     2101 agagatctcc cttcacgaca agttcgagaa catcggcgca aagctggtca aagaggtcgc
     2161 ccagaagacc caggacaaga ccggtgatgg gacaacaacc gcaacgcttc tcgcccaggc
     2221 gattctcacc gaaggcctga agaatattac ctcgggggcc aacccgatag agataaaaag
     2281 gggcattgat actgcaattg cagcggttgt agagtacatc ggctcgacca gtgtgccggt
     2341 caaggggaga gaggggattc ttcaggtggc gacggtatcg gccaacaacg atgaagagat
     2401 tgggaagctc atctcggatg ccatggagaa ggtcggctac ggcggcctca tcacggtaga
     2461 ggatgcaaag agcctcgaga ccagtctcga tgtcgtaaaa gggatgcagt ttgaccgggg
     2521 gttcatctct ccctatatgg tgacagacca cgagaagatg acgtgcgagt atgaggaccc
     2581 caacatcctg attaccgaca gaaaaatcac ttctctcaaa cagatgatcc ccgtcctcga
     2641 aatggcctcc caggaaggaa aaccgctcct tatcattgcc gaggatgtgg aaggagaagc
     2701 cctggccgcc ctcatcctga atatcatccg ggggtccttc aaggtctgtg ctgtcaaggc
     2761 accagggtat ggagaggaac gaaaagccat cctcgaagat atcgcaatcc tcaccggggc
     2821 gacggtcata tcagaagagc gtggcatgaa acttgaaaat gtatcccgcg ccgcactggg
     2881 atccgctcac accatcaaga tcgatggaga gaagaccctt atcgtcgaag gaaagggaga
     2941 ccggaaagcg ctcgatgacc ggatgcacct gatcgagtca cagatcaata tcacagattc
     3001 tgagttcaag aaagaagaac tgaaaaagcg acttggaaat ctgagtggcg gtgttgcggt
     3061 gatcaaggtg ggtgctgcaa ccgagaccga gttgaaagag aagaagatgc gaatcgatga
     3121 tgcgctgaac gccacaaagg cagccgtaga ggaaggggtg gtcccgggtg ggggcgttac
     3181 gctcttccgt gccatcgaat cccttgacag gttatccttc gatgacgaca ggaagattgg
     3241 catggctatt gtacggcgtg ccctggaaga gcctctcagg cagattgcaa agaatgcagg
     3301 ggttgagggt gcagaagtca tcgccatgat caagggacgc gaagaaaatg ggtttgggta
     3361 taatgcaaag accggcacct acgaaaacct catggagcat ggggtcatcg atcctgccaa
     3421 ggtagtcagg atcggtctcc agaatgcggg gtcgatttca ggcatgatcc tttccaccga
     3481 ggttgccatt accgatttcg atgaggagaa agataagcag agtgcggcca ttatcatctg
     3541 aattcaggaa agaagatggc agggatcttc caattccctc aagtctgtat tttttgccgc
     3601 acggtcattt cactataaaa acaccttttc acttttccat ccgcatttct gcgcgagcag
     3661 aaaaatgact gatttacggg ctccgttgac aacccggctg gggcttcaga aaaaagtatt
     3721 cttaatctct aaataggaga tttaaactat tttttcagat ggcacttccg ggcctcatag
     3781 cgggacgcta gcggacgttc cctctctgcc ctgtaagatg gcgatgagac caagcggtgg
     3841 gtgaccttca atcacaagcc cgatgagcag gcctctctcc tgaagcagat tgccgaggcg
     3901 gaggagcaga agaagatgag ctatttcatc tcggtgcccg ggtgttttta tgagattgag
     3961 tacgggctgg tgaaaggatg gaagaggggg tgatctcctc ttctccctca cttccgggta
     4021 gtttaagcgc atagtccgag cgcatctagg atcgttctc
//
LOCUS       DAOQYR010000145         3990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_23123_length_3990_cov_7.818806, whole genome shotgun sequence.
ACCESSION   DAOQYR010000145 DAOQYR010000000
VERSION     DAOQYR010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23123_length_3990_cov_7.818806"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PKM87_11440"
     CDS             <1..329
                     /locus_tag="PKM87_11440"
                     /inference="COORDINATES: protein motif:HMM:NF026098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HNQ30724.1"
                     /translation="LESLGRYIDVPEQDLEAYAVISAMGPTYLWFQLYELEQIAVSFG
                     LSPERAADAVRKMAEGTVQTMMWSHLTPEQVMDLVPVRPLGEDEPAIKEMYRRRLPAL
                     YQKLKG"
     gene            372..767
                     /locus_tag="PKM87_11445"
     CDS             372..767
                     /locus_tag="PKM87_11445"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HNQ30725.1"
                     /translation="MDYDDRPVLLLTCSGISHTGQLTTMAGNSLRCRHPILVARHVRL
                     TSLTRSLEKDLTGDECLIAVDGCEECCAKKRMDLIGKIPDAYLIATREGIIKRGMEEP
                     RYDEIECLCQAVYRSIRNDSCQEKGNDLQ"
     gene            764..1231
                     /locus_tag="PKM87_11450"
     CDS             764..1231
                     /locus_tag="PKM87_11450"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HNQ30726.1"
                     /translation="MKTKAFFIIPIILLILVLAVLAAGCMTPVDSNGNPGTTSSGAVE
                     KVEVFHFHRTQQCYSCRTLGAYAEETVDTYFAPEVASGKVVFGHVNIDLPENKALVDQ
                     YGPTGSSLWIGVYDKNGFHKEENVNVWYKIGNKEEYLAYLKGVIEKRLAGDLS"
     gene            1249..1962
                     /locus_tag="PKM87_11455"
     CDS             1249..1962
                     /locus_tag="PKM87_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HNQ30727.1"
                     /translation="METLGTSGIPLVAAFFIGLLMTFSPCPLATNIAAIAFISKKVGD
                     SKHTLLIGTLYAVGRMAAYIGLTALIVYAGLNIQAISLFLQEYGEKLIGPFLIIMGIL
                     MLEIVDIPSIGGQGWLQKIELYLGEKGYLGGFILGVIFALALCPFAAVLFFGMLIPIA
                     LKTGDAIFVPAVFGIATALPVIIISIVLVSGVNRVSGMMTAVQKNEKWIKWAVAAVFI
                     IVGVYYIVIVYGPMVGIGI"
     gene            complement(1983..2618)
                     /locus_tag="PKM87_11460"
     CDS             complement(1983..2618)
                     /locus_tag="PKM87_11460"
                     /inference="COORDINATES: protein motif:HMM:NF016831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ30728.1"
                     /translation="MKKMTVRLSADIFDYCKSFGQVIERFEVISLFNMVDDVHSEVCK
                     FTIKEGIPVDQIQCNYVSNLIVLQSAGREYTCLVKGTFSDDIARFVRTYGSKIEHPIV
                     FENDCLTFNMVGNPEDFHTFVSDAAEKGWGLEILSVCDYNPHVNGIFGVLTPKQREIL
                     MESYRQGYFDHPRRINASELAEKMGMHKTTLLEHIHKAEKRLIGHILAQVA"
     gene            2852..3133
                     /locus_tag="PKM87_11465"
     CDS             2852..3133
                     /locus_tag="PKM87_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30729.1"
                     /translation="MGILVDYVFPGFLIGLGLGFLASGLLPLVRKPRESEGINLGNTD
                     VTMLLIGAFLVFIGIGIVYAPAALWPYAIAGFLILVGIWVLIKGFYHIG"
     gene            3338..3730
                     /locus_tag="PKM87_11470"
     CDS             3338..3730
                     /locus_tag="PKM87_11470"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNQ30730.1"
                     /translation="MILQSWGIGISLYEVPPLFQVIGLVSWCFGFSLLYIGRFEMGRS
                     FRLGTPKEHTSLKVDGLFRLSRNPMYVGMYATIIAAALYTLNPVVIVLGAFVIAVHHS
                     IVLAEENHLQKVFGQEYVKYRNGVRRYV"
BASE COUNT          906 a          995 c         1068 g         1021 t
ORIGIN      
        1 tcctggaatc cctcgggcgg tacatcgacg tccccgagca ggacctggag gcctacgctg
       61 tgatctccgc catggggccg acctacctct ggttccagct ctatgaactc gaacagatcg
      121 cggtctcgtt cggtctctct ccggaacggg ctgctgatgc agtccggaag atggccgagg
      181 ggaccgtgca gaccatgatg tggtcccatc tcacccccga gcaggtcatg gacctcgtcc
      241 cggtccggcc tctcggcgag gatgaaccgg cgatcaaaga aatgtaccgg agacgcctcc
      301 cggcccttta ccagaagctg aagggctgaa ccattttgat tacagaccgt gacactccag
      361 gaggatccag catggactat gacgaccggc cggtccttct cctgacctgc tccgggatct
      421 cgcataccgg gcagctcacc acgatggcag ggaactcgct ccgatgccgt catcccatcc
      481 ttgtcgcccg tcatgtgagg ctcacctccc tcacgcggtc cctggagaag gatttgaccg
      541 gggacgaatg cctcatcgcg gtagatggct gcgaggaatg ctgtgcgaag aaaagaatgg
      601 acctgatcgg taagattccg gatgcttatc tcattgccac aagggagggg ataatcaaac
      661 gagggatgga agagccgcga tatgatgaga tcgagtgtct ctgccaggcc gtttacaggt
      721 ccatcaggaa cgattcatgt caagaaaaag ggaatgatct acaatgaaga ctaaagcatt
      781 ttttatcata cctatcattt tgctgatcct tgtgctggcg gtccttgccg ccggatgtat
      841 gacaccggtt gattccaacg ggaatcccgg gaccacctct tcaggagcag ttgaaaaagt
      901 ggaggttttc catttccatc ggacgcagca gtgctattcc tgcaggactc tgggtgcgta
      961 tgcagaggag acagtagata cctattttgc cccggaggtt gcctccggga aggtggtgtt
     1021 tggccacgtg aatatcgatt taccagagaa taaagcgctg gtggatcagt acgggccgac
     1081 cgggtcctcc ctctggattg gggtctacga caagaacggg ttccataagg aagagaatgt
     1141 caacgtctgg tataagatcg ggaacaagga ggaatacctg gcttacttga agggagtcat
     1201 cgagaagcga ctggcaggtg acctctcctg atcgacttca tgggcagcat ggagacactg
     1261 gggaccagcg gcatccccct ggtcgccgct ttcttcatcg gtcttttgat gacattctct
     1321 ccctgtccgc ttgccaccaa catcgctgcg attgcattca tctccaagaa ggtcggtgac
     1381 agcaaacata cgcttcttat cgggaccctc tatgccgtgg ggaggatggc tgcctatatc
     1441 ggcctcactg ccctgattgt gtatgcagga ctgaatatcc aggccatctc gcttttcctc
     1501 caggaatacg gtgagaagct catcggcccg ttcctgatca tcatggggat cctgatgctt
     1561 gaaatcgtgg acatcccctc catcggaggc cagggatggc ttcagaagat cgagctctat
     1621 cttggggaaa agggatacct tgggggcttc attctcgggg tcatcttcgc ccttgccctc
     1681 tgcccattcg ccgcggtcct gtttttcggg atgctcattc cgattgcgct gaagaccggg
     1741 gatgcaatct tcgtcccggc ggtctttggt atcgcgaccg cccttccggt catcatcatc
     1801 tcaatcgtgc tcgtctcggg agtgaaccgg gtttccggga tgatgactgc ggtacagaag
     1861 aacgagaagt ggatcaagtg ggcagtcgct gccgtgttca tcatcgtcgg agtgtactat
     1921 attgtcatag tatacgggcc gatggttgga ataggaatat gaaggacggt tgatcggatc
     1981 gcttaagcaa cttgtgcgag gatatgcccg atcagccgct tctccgcctt atggatgtgc
     2041 tcaagcagcg tggtcttgtg cattcccatc ttttccgcaa gttcactggc attgatcctc
     2101 cggggatgat cgaaataccc ctggcggtac gattccatca ggatctctct ctgtttggga
     2161 gtaaggacac caaaaattcc gttaacatgc gggttataat cacagaccga aaggatctca
     2221 agtccccacc ctttctccgc agcatctgac acgaacgtat ggaagtcctc agggttcccg
     2281 accatgttga atgtcaggca atcattctcg aagacgatcg ggtgctcgat ctttgacccg
     2341 taggtacgaa cgaacctcgc aatatcatcg gaaaaagttc ccttgacaag acaggtatac
     2401 tctcgacctg cgctctgaag cacgatgaga ttcgacacat aattgcactg gatctgatcg
     2461 actggtatcc cttccttgat ggtaaacttg cacacctcgc tgtgcacatc atcaaccatg
     2521 ttgaagaggg agatcacctc gaaccgttcg atgacctggc caaaggattt gcagtaatcg
     2581 aagatatccg cggagagcct gaccgtcatc ttcttcatac actgcgttct ggtacgcaag
     2641 tggtgatata aatatggcca tacatgtatg gctcaattca taacaccttt gcctgcctct
     2701 tcttattcag acattccacg aggaatgatg catgaacaca aacaattgca ctgaaatcaa
     2761 agaggctgag agaagaggac tgacggaatc cggggaacac cacaagcctt tcgaattcat
     2821 tattcctgcc ggtatcctga tcggtttggg cgtgggtatt cttgttgatt atgtatttcc
     2881 cggattcctt atcgggctgg gactcggatt tctcgcatca ggactgcttc ccctcgtcag
     2941 gaaaccgcgt gagagtgaag gtataaacct ggggaacacc gatgtgacga tgcttctcat
     3001 cggagctttc ctggttttca tcgggatcgg cattgtctat gcgccagctg cactctggcc
     3061 ctatgccatt gccggatttt taatccttgt tggtatctgg gtcctgatta agggattcta
     3121 ccatatcgga tagggatagg acgggagaac aaattcctgg gtgactggca tgatcgaggc
     3181 tcttttggca acgctcctcc cccttggttt ccttattgtg ttattcggcg gaggggcgca
     3241 ctttttgcgg aacaatattg aacaggacgg ggaggctcca ataaaccgga cactgtttta
     3301 cgtcagcaaa tattcgatcc ttgttctgtg gggtgcgatg atcctccaga gctgggggat
     3361 tggcatatcg ttgtatgaag tgccccctct cttccaggta atcggcctgg tatcatggtg
     3421 tttcggattt tctcttttat atatcggaag attcgaaatg gggaggtcat tccgcctggg
     3481 caccccgaag gaacacacca gtctcaaggt cgatggatta ttcagattga gcagaaatcc
     3541 gatgtatgtc ggtatgtatg caacgattat tgctgctgcc ctctatacgc tgaatccggt
     3601 tgtcattgta ctgggagcgt tcgtgattgc ggtccatcat tccattgtcc tggctgagga
     3661 gaaccatctg cagaaggtgt ttggacagga gtatgtgaaa tatcgcaatg gggtaaggcg
     3721 gtatgtctag ctgaggaagg cggacgtatt gaggaacggg gatggtgatg ctattatcga
     3781 gcctcagtat tgttttggat ttgttcgaaa atatttcatg tcgcccggtt atttcactat
     3841 aaaaaccacc ttacactttt tcacccgcat ttctgcgcga gcgaaaaaat aactgattta
     3901 cgggctccgt tgccgatccg gctggggctt taaatataat ttggacagat ggcaaattga
     3961 tttttatttc tgaaaataaa gctaaatttt
//
LOCUS       DAOQYR010000146         3888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_23992_length_3888_cov_9.297156, whole genome shotgun sequence.
ACCESSION   DAOQYR010000146 DAOQYR010000000
VERSION     DAOQYR010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23992_length_3888_cov_9.297156"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..324)
                     /locus_tag="PKM87_11475"
     CDS             complement(43..324)
                     /locus_tag="PKM87_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30731.1"
                     /translation="MHCEDPGTARRAYMKAASEPGVPRRGREDLHETADRWLRARRAV
                     PAADQPHAERLAEMIRAHATDRLAAIEDPLEAAMFSLLIELVKEHQRGQ"
     gene            479..925
                     /locus_tag="PKM87_11480"
     CDS             479..925
                     /locus_tag="PKM87_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HNQ30732.1"
                     /translation="MEGCIKLGIIICDRYRTCAGGKCLRALRNREGAFERYRGRDVEL
                     VGFATCNGCPGGNVEYVPQEMKKNGAEVVHLATGLVVGYPPCPRIGYFSRFIREKYGM
                     DVVVGTHPIPQNYYATHANLGTWDSAAWKEMLAATLTDEKTRLSYD"
     gene            1028..2194
                     /locus_tag="PKM87_11485"
     CDS             1028..2194
                     /locus_tag="PKM87_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04083 family peptide-modifying radical SAM
                     enzyme"
                     /protein_id="HNQ30733.1"
                     /translation="MKNAFHVMLVPTLGCPARCRYCWSSDPASPRMSIGTLSEVVDWL
                     KGIEKERVTFTFHGGEPLLAGADFYRQALPLLSRELSHLNPEFAMQSNLWLMTPEIAD
                     ILAEYHVPLGTSIDGPEELNDTQRGEGYFQKTMKGYAIAREHGLKVSFICTFTSRSFH
                     ERERIVRFFMENGFTLKLHPALPSLRNDNPEPWTLDPADYGELLVYLLDTSLEHLGRM
                     EIMNISDLCKCVFFRHGTVCTFVDCMGSTFAVGPDGGIYPCYRFVGMPEYVMGNVRDR
                     PTLDELADSHIGRLMTEFRHLVDQSCAECAHVRYCRGGCPYNAMVPHGGEIKGVDPYC
                     TAYRRIFDEITSRLNREMAESLPFEMGPFQPARQKDRRPGIMALMRYQAMKDPR"
     assembly_gap    2436..2535
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2553..3590)
                     /gene="mtrH"
                     /locus_tag="PKM87_11490"
     CDS             complement(2553..3590)
                     /gene="mtrH"
                     /locus_tag="PKM87_11490"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit H"
                     /protein_id="HNQ30734.1"
                     /translation="MFRFEKEQKVWDFNGTMIGGQPGEYPTVLGASIFYNKHEVVLDD
                     HKGKIDEKKAEALWNRCQELSDMTGIPHFIQIIAEFGEAFESYFTWFDKIDNKTAFLM
                     DSSVPAALAHACKYVTEVGLAKRAIYNSINGSIPPENVEAIKNSDVDAAIVLAFNPAD
                     PSVAGREKTLVEGGVAGQTKGMIQIAEEAGITRPILDTAATPLGLGSGGSYREILACK
                     AIHGFPTGGAYHNMTVSWTWLKRWKGSKKNPSKLAESLKGKDAYLEQLLHHYQGGLDG
                     VIQAAWSAPDIGCNMIASTLGADLIMYGPIENVEAMITAQAYTDMVVLEATRQMGIEC
                     KAESHPIFKLI"
     gene            complement(3608..>3888)
                     /gene="mtrG"
                     /locus_tag="PKM87_11495"
     CDS             complement(3608..>3888)
                     /gene="mtrG"
                     /locus_tag="PKM87_11495"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit G"
                     /protein_id="HNQ30735.1"
                     /translation="DPGAFAADPMVVEVKEAAGGVEVAAAGVNPQFLEIEKRLDKVEK
                     QIEFADAEIAQRVGRKIGRDIGILYGLVAGLMVFVMLLVLLPKLNVMM"
BASE COUNT          741 a         1111 c         1156 g          780 t
ORIGIN      
        1 ccctccggac cacccccgca caacccccgc accagcgggg gcttattgcc cccgctggtg
       61 ctccttcaca agctcgatga ggagcgagaa catcgccgcc tccagggggt cctcgattgc
      121 ggcgaggcgg tcggtggcgt gggcccggat catctccgcc agccgttcgg cgtggggctg
      181 gtcggctgcg gggaccgccc gccgggctcg cagccagcgg tcagcggtct cgtgcaggtc
      241 ttcgcgtccc ctgcggggta ctcccggttc ggatgcggct ttcatgtagg ctctcctggc
      301 agtgcctgga tcttcgcaat gcatcaaagg ggaggcaggg ggggtgaaag tgaggagggg
      361 gatgcctggc ggcacgcaat gttctcaacc gaccgggaat ctcccgtgct gcgttcccag
      421 catatttctg cgatgaatgc caatatccag gcaacgtgag ttgaggagtt ggatctccat
      481 ggaaggatgc attaaactcg gaatcatcat ctgcgaccgc taccggacct gtgcgggggg
      541 gaaatgcctc cgtgcgttgc gcaacaggga aggtgcgttc gagcggtaca ggggcaggga
      601 tgttgaactt gtcgggtttg ccacctgcaa cgggtgtccc ggaggaaacg tcgaatacgt
      661 gccgcaggag atgaagaaga acggggcgga ggttgtccat cttgcgactg gcctggtggt
      721 cgggtacccc ccctgtcccc ggattggata cttctcgcgg tttatccgtg aaaaatacgg
      781 catggacgtg gtcgtcggga cacaccccat tccccagaac tactatgcga cccatgcaaa
      841 ccttggcacc tgggattccg cggcgtggaa agagatgctt gcggcgacac tcaccgatga
      901 gaagacgcga ctctcatatg attaggctca aggcaagtcc aggatctgcc ggactctgca
      961 aaaaccctct ggtcccgggt gcaggaaagg caatccttac cacccccggg gagaaacctc
     1021 tcctgtcatg aagaatgcct ttcacgtgat gcttgtcccc acgctgggct gccctgccag
     1081 gtgcaggtac tgctggagct cagatcccgc atctccacgg atgagcatcg ggaccctcag
     1141 cgaagtggtg gactggctga aaggcatcga gaaagagcgg gtgacgttca cttttcacgg
     1201 cggcgagccg ctcctggcgg gtgctgactt ttatcgccag gcactccccc tcctctcccg
     1261 ggagctctcg cacctgaacc ccgagttcgc catgcagtcc aatctctggc tgatgacccc
     1321 tgagatcgcg gacatcctgg cggaatacca tgtgccgctt ggaaccagca tcgacggccc
     1381 ggaggagctc aacgataccc agagggggga aggctacttc cagaagacga tgaaggggta
     1441 tgcgattgcg cgggagcacg ggttgaaggt gagtttcatc tgcaccttca cttcgcggtc
     1501 gttccacgag agggagagga tcgtccgttt cttcatggag aacggcttca ccctcaagct
     1561 gcaccccgcc ctcccgtcgc ttcggaacga caaccccgag ccctggacgc tcgatcccgc
     1621 cgattacggt gagcttctgg tctatctcct cgacacgtcc ctcgagcacc tggggagaat
     1681 ggagatcatg aatatcagtg acctgtgcaa gtgcgtcttc ttccggcatg gaacggtctg
     1741 cacgtttgtg gactgcatgg gaagcacctt tgcggtcggc ccggatggcg gtatctaccc
     1801 ctgttaccgg ttcgtgggta tgccggagta cgtgatggga aatgtgcgcg accgtcctac
     1861 actcgacgag ctggcagact cgcacatcgg gcggctcatg acggaattcc ggcaccttgt
     1921 tgaccagtcg tgtgcagaat gcgcccatgt caggtactgc cgcggagggt gcccctataa
     1981 cgccatggtg ccgcacgggg gagagataaa gggggtggac ccctactgca ccgcgtacag
     2041 gcgcatattc gacgagatca caagccgact caacagagag atggcggagt ccctgccgtt
     2101 cgagatggga ccgttccagc cggcacggca gaaggatagg agacccggca tcatggcgct
     2161 gatgcggtac caggcgatga aagacccaag atgaggaacg caagatgcat gcagatgccc
     2221 aaaggcatct cccatttggg ggcattcaca ggcccggagc caactctgac cggagcgtca
     2281 ctcaattttt caaaaatagc gggtagtgga aaagagcact ggggaaggtt tgcgtaaacc
     2341 cgaatgcaca cgcctctggc gtggtaaggt gtcaggacca ggaaaagagc actggggaag
     2401 gtttgcgtaa acccgaatgc acacgcctct ggcgtnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnngtgca tataaaaaaa atttaaatga gtttgaatat ggggtggctc
     2581 tcggccttgc actcgattcc catctgccgg gtggcttcca gcacgaccat gtcagtgtat
     2641 gcctgggccg tgatcatggc ctccacgttc tcgatggggc cgtacatgat gaggtcagca
     2701 ccaagcgtgc ttgcgatcat gttgcagccg atatcgggtg cagaccatgc agcctggatg
     2761 accccgtcaa gtccgccctg gtagtggtgc aggagctgct cgaggtaggc atccttgccc
     2821 ttcagggact cggcgagctt ggacgggttc ttcttcgagc ctttccagcg cttcagccag
     2881 gtccaggaga cggtcatgtt gtggtacgca ccacctgtgg ggaacccgtg gatggccttg
     2941 caggccagga tctcgcggta tgaaccgcca gagccgagcc cgaggggggt tgccgcggtg
     3001 tcgaggatgg gcctggtgat tcctgcctcc tcggcaatct ggatcattcc cttggtctgg
     3061 ccagctacac cgccctcgac gagggtcttc tcccttccgg ccacggaggg gtcggcgggg
     3121 ttgaatgcca gcacgatggc ggcatcgacg tcgctgttct tgatggcctc gacattctcc
     3181 ggcgggattg acccgttgat ggagttgtag atggccctct ttgcaagccc gacttccgtg
     3241 acgtacttgc aggcgtgcgc gagtgctgcc ggcaccgacg agtccatcag gaacgcggtc
     3301 ttgttgtcga tcttgtcaaa ccaggtgaag tagctctcga acgcctcccc gaactctgca
     3361 atgatttgga taaagtgggg gatcccggtc atgtcggaga gttcctggca acggttccag
     3421 agcgcctccg ccttcttctc atcgatcttg cccttgtggt cgtcaagcac tacctcgtgc
     3481 ttgttgtaga agattgacgc accgagcaca gtcgggtact ctccaggctg gccaccgatc
     3541 atggtgccat tgaagtccca gactttctgt tctttctcaa acctgaacat ctgaattcac
     3601 acctccttca catcatcaca ttcagcttcg ggagcaggac cagcagcatg acgaatacca
     3661 tcagtccggc gaccagtcca tagaggatgc ctatatcccg gccgatcttc ctgcccacac
     3721 gctgggcgat ctctgcatcg gcgaactcga tctgcttctc gaccttgtcc agccgcttct
     3781 ctatctccag gaattgcggg ttgaccccgg ctgcggcgac ttcgacgccg cctgcggctt
     3841 ccttgacctc cacgaccatg gggtctgcgg caaatgcgcc cgggtcac
//
LOCUS       DAOQYR010000147         3854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_24316_length_3854_cov_7.748618, whole genome shotgun sequence.
ACCESSION   DAOQYR010000147 DAOQYR010000000
VERSION     DAOQYR010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24316_length_3854_cov_7.748618"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            683..907
                     /locus_tag="PKM87_11500"
     CDS             683..907
                     /locus_tag="PKM87_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNQ30736.1"
                     /translation="METFRFSVVIEKDESGYYVFCPELQGCYSQGSTYEEALDNIRDA
                     IRLHLEDRISCGEAVPQPKMVSITTLDVAV"
     gene            904..1125
                     /locus_tag="PKM87_11505"
     CDS             904..1125
                     /locus_tag="PKM87_11505"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNQ30737.1"
                     /translation="MKGKLPRVTAEKIIKILHTHGFIEVRQSGSHKIFRNSQGCRVTV
                     PVHSGTILHPKLIKAILEDAGISPEELEK"
     gene            1239..2528
                     /locus_tag="PKM87_11510"
     CDS             1239..2528
                     /locus_tag="PKM87_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNQ30738.1"
                     /translation="MPPSTQPKLARRLGFWEVTLSGIGIILGAGIYALIGQAAGSAGN
                     AVWLSFVFSALVAFFTALSYMELSSMMPDVGAEYEYTARAVGRRMAMVIGWLIIFSGI
                     LGAATVSLGFAGYLGGLLPIAVFPAAVLLIAVLCGILLLGVKESAWVAVVFTLIEVGG
                     LLLIIAAGIPCLGRVDYFEMPLGLSGVVTAAALVFFAYQGFEEMVKFSEETRRPERTV
                     PLALITALVVSTVLYIFVCISAVSVVGWEALAASSAPFATVASAAWGADASLLLSVIA
                     LFATANTVLMMMFASSRISFGMARAGSLPGLLSRVHPGRHTPWTAILLVGGGALFFMF
                     TGDIAFVANIANFTLFVTFVVVNLSVIILRYREPDRPRPFSIPGRLGNFPVFPLLGLL
                     SCIFLLVQLEPAVLGVGALLTGIGVVIAIFYGEVEER"
     gene            2696..2956
                     /locus_tag="PKM87_11515"
     CDS             2696..2956
                     /locus_tag="PKM87_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNQ30739.1"
                     /translation="MILEYIHAALDRAQYEIIEDEEPFYGEIPDLPGVWATGKTLEDC
                     RQKLKEVLDGWIIIHLQRGLPIPALGEYTIEEMTGLDSIVRA"
     gene            2857..3171
                     /locus_tag="PKM87_11520"
     CDS             2857..3171
                     /locus_tag="PKM87_11520"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNQ30740.1"
                     /translation="MDYHSSAKGTSYPRIRRIHDRRDDWTGFYCQSISSSLSNGRNLV
                     RKLRVLGFEGPYRGGKHPFMIKDALVLTLPNPHRQKIGPDLLAKILRHAGISHDEWVR
                     TG"
     gene            complement(3232..>3854)
                     /locus_tag="PKM87_11525"
     CDS             complement(3232..>3854)
                     /locus_tag="PKM87_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30741.1"
                     /translation="FVEGKAPGPVDNRLLFYRENQARIPAICNGIVPDPISSVADYRE
                     RLSGVYAELRSLGAGILCEEWVASAGVIVRFSRPCIEIKAIDEQECVFSDMALCAFVR
                     ALARARDLPIEEDRDSLVAMTESAIRSGTDGLEDELAALYRRAAKAATADERRYLPLV
                     RERIENGSLGQVLAERFRDTGDLQGIMNDLAMCLVENRPYVGNGIV"
BASE COUNT          765 a         1095 c         1097 g          897 t
ORIGIN      
        1 gtgcttaccc aagcacagga aaccccggta ctagaaccct caaatttttc gaacttgctc
       61 ttctgtagaa tttattgacg cggtcctgcg tcgttgtgtg tgtttttggt ggtgtgtgtg
      121 cgatagtgag cggaggcggt aggctgtttt tttactgtaa ttcaatcttc gtggtttcct
      181 gattggttta ataaaaggaa aggccgccct tgaggacaga gttcaaggtt ataattggtg
      241 gggaggggga gaatcccaat ccttcggatc cccgggcgag gcgccctttt cccttgcccc
      301 ctcccccgat acgggatagc ccatcggcag agttgcatcg gtataccaat cgatacttac
      361 tgctcccccg cactggtcct atcgcgcctt aggggacgcc acagcggcgg ggcgtaagcc
      421 ccaggagcgt caccgtgtga tgagtgcggg cggatttacc tcatgaacta tacttgggca
      481 agtgtctgta caaccccccg cagtcggggc tgattcctca actgttctgc cattgtgtga
      541 tgagtgcggt tggatatcac ccacgaacag accgaaccag ggcattggcc gtcggccccc
      601 tgcagcaatg acctcgcggt tctttctgct ccccggggta tcattattag gagagactat
      661 ccacatctga ttgggcaaaa taatggagac atttcgtttc tctgtcgtga tcgagaagga
      721 cgagtcaggg tattacgtat tctgccctga gcttcagggg tgttattcac agggatctac
      781 ctatgaagag gcccttgata atatcaggga tgcaatacgc cttcatctgg aagatagaat
      841 ctcatgcggc gaggcagttc cccaaccaaa aatggtgagc attaccactc ttgatgtggc
      901 agtatgaagg ggaaattgcc acgggttaca gccgaaaaga tcattaaaat attgcataca
      961 catggtttta tcgaagtccg tcagagcgga agtcataaga tatttcgcaa ttcacagggg
     1021 tgcagagtca cggttccagt tcattcggga acaatccttc atcccaagtt gataaaggct
     1081 atcctcgaag atgcgggaat ttcacctgaa gaacttgaaa aataaaatta tattgcgttg
     1141 gattgttgcg cgagcttgtt ttctttatta aggacgaaaa ccctcatcct ctcccacccc
     1201 ctaacatctc tcaaagccag gtgatcctta tcgcaacgat gcccccctcg acgcagccga
     1261 aacttgcccg aaggctcggg ttctgggagg tgaccctctc ggggattggg atcatcctcg
     1321 gcgccgggat ctacgcgctc atcgggcagg cggccgggag cgccgggaac gcggtctggc
     1381 tctcgttcgt gttctcggct ttggttgcgt tcttcaccgc cctctcctac atggaactct
     1441 cctcgatgat gccggacgtc ggcgccgagt acgagtatac tgccagggcg gtgggccgcc
     1501 ggatggcgat ggtgatcggg tggctgatca tcttctcggg catcctcggt gcggcgacgg
     1561 tgtcactcgg gttcgccggg taccttgggg gcctgctccc catcgcggtc tttccggccg
     1621 ccgtgctgct gatcgcagtc ctctgcggga ttcttctgct cggagtgaag gagagtgcat
     1681 gggtggcggt cgtgttcacc ctcatcgagg tgggcgggct cctgctgatc atcgcagccg
     1741 ggattccctg cctcgggcgg gtggactact tcgagatgcc gctcggcctc tccggcgtgg
     1801 tcaccgccgc ggcgcttgtc ttcttcgcct accagggatt cgaggagatg gtgaagtttt
     1861 cagaagagac gagaaggccg gagcggaccg tcccgctcgc cctgatcacg gcgctcgtgg
     1921 tctcgaccgt cctctacatc tttgtctgca tctcggcggt gagcgtggtt ggctgggagg
     1981 cgctcgcggc atcctccgcg ccgtttgcga ccgtcgcgtc cgccgcatgg ggggcggacg
     2041 cgtccctcct cctctcggtg atcgcgctct ttgcgaccgc caacaccgtg ctgatgatga
     2101 tgtttgcctc ctcgcggatc tcgttcggga tggcccgggc aggctcgctc cctggactcc
     2161 tctcgagggt ccaccccggc cggcacaccc cgtggactgc gatcctcctc gtcgggggag
     2221 gtgcgctctt cttcatgttc accggggaca tcgcgttcgt cgcaaacatc gccaacttca
     2281 ccctctttgt cacgttcgtg gtggtgaacc tctcggtgat catcctccgc tacagggagc
     2341 ccgacagacc gcggccattc tccatccccg gcaggctggg gaattttccc gtctttccgc
     2401 tcctcggcct cctctcctgc atcttcctgc tggtgcagct cgagccggcc gtgctcgggg
     2461 tgggggcgct cctcaccggg atcggggtgg tgatcgcgat cttttacggg gaggtggagg
     2521 agaggtgaga ggggatacta ttgcatgaca taagatagca gagaaaacga atttgtaaaa
     2581 aaaaacacca cgcattccgt ttttggatct tgtcgggagg ggagatttac ttccacttcg
     2641 ttctcctttc ccgcagtagt cttattacaa ccaacaacag attgaggaac aatcaatgat
     2701 acttgaatac atacatgccg cgcttgaccg cgcacaatac gagattatcg aagacgaaga
     2761 gccattctat ggggaaatac cagatttacc cggggtatgg gcaacgggaa agacgctcga
     2821 agattgccgt cagaaactaa aggaggttct tgatggatgg attatcattc atctgcaaag
     2881 gggacttcct atccccgcat taggagaata cacgatcgaa gagatgactg gactggattc
     2941 tattgtcaga gcataagctc cagcctgtca aatgggcgaa atctcgtgag aaaattacgc
     3001 gtattgggat ttgaaggtcc ttaccgcggc ggtaaacacc cttttatgat taaagatgct
     3061 ctggttctta cgctccccaa tccgcatcgg cagaagattg gaccagacct ccttgcgaaa
     3121 attctccggc acgcaggaat ctcgcatgat gagtgggtga gaacaggcta attatacgaa
     3181 attccttttt tccattcttc gatttcataa attcctgcaa cattgagatt atcacacgat
     3241 gccattcccc acatatggcc ggttctccac caggcacatc gcaaggtcat tcatgatccc
     3301 ctgcaggtcc ccggtatcgc ggaacctctc cgcgaggacc tgcccgaggc tcccattctc
     3361 gatcctctcc cttaccagcg gcaggtaccg ccgttcatcg gcggtcgccg ccttcgcggc
     3421 gcgccgatag agtgcggcga gttcatcctc gaggccatcg gttcccgacc ggatggcgct
     3481 ctccgtcatc gcgacgagcg agtcccggtc ctcctcgatg ggaaggtcac gggccctggc
     3541 gagcgcccgc acgaaggcgc agagcgccat gtccgagaag acgcactcct gttcgtcgat
     3601 cgccttgatc tcgatgcagg gacggctgaa ccggacgatc acgcccgcgg atgccaccca
     3661 ttcctcgcag aggatacctg ccccgagcga ccggagctcg gcgtagaccc ccgagagccg
     3721 ctcccggtag tccgcgacag acgagatggg atcagggacg atcccgttgc agatggccgg
     3781 gatcctggcc tggttctccc ggtagaagag gaggcggttg tcgaccggcc cgggggcctt
     3841 cccctcgacg aacg
//
LOCUS       DAOQYR010000148         3793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_24891_length_3793_cov_4.579989, whole genome shotgun sequence.
ACCESSION   DAOQYR010000148 DAOQYR010000000
VERSION     DAOQYR010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24891_length_3793_cov_4.579989"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..1178)
                     /locus_tag="PKM87_11530"
     CDS             complement(243..1178)
                     /locus_tag="PKM87_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HNQ30742.1"
                     /translation="MFIPGAYALNVVSTTTVLWDPVQYIGGEKVHAIYIADPTICPHM
                     QADIIPNRMQMQRDFIRGADLFIAHNGSVDVSYVMPFVDDFMEANHFGTVKWVTLKNP
                     SMIWNTPDGAKALSQEVAGWLIAADPANREYYESRLAEYLAQIGAADISTEERKRIAG
                     QDIVVMVWQQEAAGKWLGMDTVSIYAPEFYMGGKFTPVKIVDDINANITKYQEVRYII
                     ENMQSGELAKGLEEALHTKGIPAKRVIFTNFPKSIPGVDTLPDVIRYNKALVTPEDGI
                     TGQPTSAPTTTPLSCVVVAGGILGAFLLFLNKRED"
     gene            complement(1246..2076)
                     /locus_tag="PKM87_11535"
     CDS             complement(1246..2076)
                     /locus_tag="PKM87_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNQ30743.1"
                     /translation="MLEFLIPNTIVCHAVEAMLFASVACAILGVIITQIQISSIGFTM
                     AHAAFAGAAVGMFFSLNSTICAILAGICVAFLIGPLSEKARVSADTALGLLFGISMAV
                     AIFFIAYMQTLGKGFSAMVILFGDVISLYQGEIYLLGVICVVAVVFVVMLYKEITAII
                     FNKKIAESTGVRVRPVYYAMLFVIALTVALCIPIIGGLLLYVWLVTPAAIALQFCYRL
                     SVMFVVAPLVAAAVSVSGALAGLEYSLPVAPLTAVLFSLVFIVAVVLSPKRRVSFRKF
                     "
     gene            complement(2069..2818)
                     /locus_tag="PKM87_11540"
     CDS             complement(2069..2818)
                     /locus_tag="PKM87_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HNQ30744.1"
                     /translation="MACADPVIEIKGVYTAYEGADRPTLHDVSLTVGRGEYVVVGGPN
                     GAGKTTLLETINGMLPIIHGEAHVCGHSVKGNGVEVRCRVGYLLQNFSFDSLTPFTVE
                     EVVLMGRFGKIGLFRKPGKEDRDAAMKALGILGIEDLASRPIGQLSGGQQQKVLLAQN
                     LARDPEILLLDEPFSNLDMFARESINRLLSRMVAAGVTVIIVSHAFDDLPDRTVRVLV
                     MQEGKIVLDRQCSPREVEKAIRNAGNGTNNA"
     gene            complement(2826..3269)
                     /locus_tag="PKM87_11545"
     CDS             complement(2826..3269)
                     /locus_tag="PKM87_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HNQ30745.1"
                     /translation="MDAHTHSHCRYYNLNYDFTVTDLAAFHGHLGPYIVLGYRIGKYI
                     REHFCSDPFQMKAEVYCAGTPPQSCLADGVQLGSGCTLGKRNIEIVRSDTIRCVFWTN
                     GKRVTINPRPIPLPQRDMDYEAAIEGIAENMYTRPERDLFDVIQG"
     gene            3410..>3793
                     /locus_tag="PKM87_11550"
     CDS             3410..>3793
                     /locus_tag="PKM87_11550"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ30746.1"
                     /translation="MNNTERVHGVMEAVQYDHLIQAIVPGQSLLLSTIIDTLPTPCGR
                     VLELGCGTGIMTGMIRDACPDAEITAIDISCEMLEIASAKPELEGVLFLSRDLREPWP
                     AGEYDAIVSSLCLHHVSPADRQEVAR"
BASE COUNT          909 a         1037 c         1011 g          836 t
ORIGIN      
        1 atctcacggc ttttttttca cttgatagcc acgggaagtg agcactcctc ctccccattc
       61 ccggtttcac gccaaatggc ggcattttac ttgcggcgga aatgaggtga aaatccggga
      121 ttaaaagaat aaggagatgg cgcgagggac cctgccggaa atccgccaac accatgcatt
      181 ccaatgccgc attctgaaac gtgtggtcct attaaaaagt gaaccggaat cgagagagga
      241 tctcaatcct ctctcttatt caggaatagc aggaacgccc caaggatgcc ccctgcaacc
      301 acgacacacg agagtggtgt ggtggtcggg gcacttgttg gctgacccgt tattccatcc
      361 tcgggagtaa caagagcctt gttataccgg atgacgtcgg gcagggtgtc aacaccaggg
      421 atcgacttcg ggaaattcgt aaagatcacc ctctttgcgg ggattccctt ggtatggagt
      481 gcttcctcga ggcccttggc aagctcgcca gactgcatat tctcgatgat ataccggact
      541 tcctggtact tcgtgatgtt cgcattgata tcgtcgacga ttttgaccgg agtgaacttc
      601 cctcccatgt agaattccgg cgcatagatg ctcactgtgt ccatacccag ccactttcct
      661 gccgcttcct gttgccagac catcaccacg atgtcctggc cggcaatccg tttcctctcc
      721 tccgttgaga tatcagcggc accgatctgc gcgaggtact cggccaatcg cgattcataa
      781 tactccctgt tcgcgggatc tgcagcaatg agccagccgg caacctcctg cgagagggcc
      841 tttgctccat caggagtatt ccatatcatc gagggatttt tcagggtgac ccatttcaca
      901 gtaccgaaat ggttggcctc catgaaatca tccacaaaag gcatgacata cgagacgtcg
      961 acggatccat tgtgggcaat gaagagatcc gcgcccctga tgaagtctct ctgcatctgc
     1021 atgcggttcg ggattatgtc ggcctgcatg tgggggcata ttgtaggatc ggcaatgtag
     1081 atggcatgaa ccttctctcc cccgatatac tggaccgggt cccagagaac cgtggtcgtg
     1141 gagaccacgt tcagtgcata cgctcctggt atgaacaaca atatcaaaaa tgtgatgaat
     1201 agagtaaatt ttttattcat gttttcattt tttgcgagga tgacattaaa attttcgaaa
     1261 tgaaacgcgt ctctttggcg agaggactac cgcgacaatg aatacgagag agaagaggac
     1321 tgcagtgaga ggtgcaaccg gcagggaata ttcaaggcct gcaagcgctc ccgacacgct
     1381 cactgcggct gcaaccaggg gggccaccac gaacatgaca gagaggcggt aacagaactg
     1441 gagtgcaatt gccgccgggg taaccagcca tacgtagaga aggagtcccc cgataatcgg
     1501 gatgcagagt gccactgtca gcgcgatgac gaaaagcatt gcgtaataga ctggccttac
     1561 acggactcct gtcgattcgg caatcttctt gttgaagatg atcgccgtaa tctccttgta
     1621 gagcatgacc acgaatacca ctgcgaccac acaaatgacg cctaaaagat agatctctcc
     1681 ctggtagaga gagatgacgt ccccaaagag gatcaccatt gcggaaaagc cctttccaag
     1741 ggtctgcatg taggcaatga agaagatcgc caccgccatg ctgatgccaa aaaggaggcc
     1801 gagtgcagtg tcggccgaga cccttgcctt ctccgaaagc ggcccgatga gaaaagccac
     1861 gcagataccg gcaaggattg cacagattgt ggagttgaga gagaaaaaca tccccaccgc
     1921 ggcaccggca aaagcggcat gggccatggt aaaccctatc gacgagatct ggatctgcgt
     1981 gatgattacc ccgaggattg cacaggcaac cgaggcaaag agcatcgcct ccaccgcgtg
     2041 gcatacgata gtgttcggga tgaggaattc aagcattatt cgtcccgttc ccggcattcc
     2101 tgatagcctt ctccacctcg cggggggaac attgccggtc gagcacgatc tttccctcct
     2161 gcatcaccag tacccggaca gtccgatcag gaaggtcgtc aaacgcgtgg gaaacaatta
     2221 tcactgtgac tcccgcagcg accatacgcg agaggaggcg gttgatggat tccctggcaa
     2281 acatatcaag gttggagaat ggctcatcaa ggaggagtat ctccgggtcg cgggcaaggt
     2341 tctgtgcaag caggactttc tgctgctggc ctcccgagag ctgcccgatt gggcgggacg
     2401 cgagatcttc gatcccaaga atgccaaggg ccttcatcgc cgcatcgcgg tcttccttcc
     2461 ccggcttacg gaagagcccg atcttcccga accttcccat gaggaccacc tcctcgacgg
     2521 tgaagggggt gagggagtcg aatgagaagt tctggaggag gtagcccacg cggcatcgta
     2581 cctccacgcc gttgcccttc acgctgtgcc cgcagacatg cgcctccccg tggattattg
     2641 ggagcatccc gttgattgtc tcaaggaggg tggtcttacc tgcaccgttc gggcccccga
     2701 ccaccacgta ctcgcccctt ccgacagtga gcgagacatc gtggagggtc ggccggtcag
     2761 caccttcata ggccgtgtaa acccccttga tctctatgac tgggtctgcg caggccatgc
     2821 tcacattacc cttggatgac gtcgaagaga tccctctccg gccgggtata catattctcg
     2881 gcgattccct ctatggcagc ctcgtagtcc atgtcccttt gaggaagagg aatggggcgg
     2941 gggttgatgg tgaccctctt tccattggtc cagaacacgc agcggatcgt atccgaccgg
     3001 acgatctcga tattcctttt cccaagagtg cacccgctcc ccagctggac accgtcagca
     3061 agacatgact gcgggggtgt tcccgcacaa tacacctcgg ccttcatctg gaagggatcg
     3121 ctacagaaat gctcacggat gtatttgccg atgcggtacc caagcacgat atatggccca
     3181 agatgaccat ggaatgcggc gagatcagta actgtgaaat cgtagttaag attatagtat
     3241 ctgcagtggg aatgtgtgtg agcatccatg atgtaatgtc tccagattga tatttataaa
     3301 ttagcaattc tgacttggta tgaaataaaa acaattattt cctatataat tgcattatcc
     3361 gtaaccgttt agatatgaat tccagtgtat caatgcccgg aggatctcct tgaacaacac
     3421 agagagggtt catggcgtga tggaagccgt gcaatacgac cacctcatac aggcaatcgt
     3481 cccgggacag tccctgctcc tttccacgat tatcgacacc ctgcccactc cctgcggaag
     3541 ggtcctggaa ctcggctgtg gaacgggcat aatgacgggg atgatccggg atgcatgtcc
     3601 agacgcagag atcactgcaa tcgacatctc ctgcgaaatg ctggagatcg catcggcaaa
     3661 accggaactc gaaggggtac tctttctctc ccgcgacctg cgcgaaccgt ggcccgcagg
     3721 ggagtatgat gccattgtct cctccctctg cctgcaccac gtatctcctg cagaccgcca
     3781 ggaggttgcc cgg
//
LOCUS       DAOQYR010000149         3777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_25047_length_3777_cov_9.002418, whole genome shotgun sequence.
ACCESSION   DAOQYR010000149 DAOQYR010000000
VERSION     DAOQYR010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25047_length_3777_cov_9.002418"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /gene="cofH"
                     /locus_tag="PKM87_11555"
                     /gene_synonym="cofG"
     CDS             complement(<1..191)
                     /gene="cofH"
                     /locus_tag="PKM87_11555"
                     /gene_synonym="cofG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314735.1"
                     /note="7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase
                     subunit 2; catalyzes radical-mediated transfer of
                     hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to
                     5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form
                     7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO); functions
                     in F420 biosynthesis along with cofG; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase subunit CofH"
                     /protein_id="HNQ30747.1"
                     /translation="MSTGSTVAPAFLTGRTLHVLLGDIRGGHRLTPDEAAALLKVRGR
                     DVWRVANAADEQRERVVGDA"
     gene            276..578
                     /locus_tag="PKM87_11560"
     CDS             276..578
                     /locus_tag="PKM87_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YunC family protein"
                     /protein_id="HNQ30748.1"
                     /translation="MQEVKVTLLNSEGRGFVIPLGPVNLVGVVASKGLVGCGAIDVAA
                     LEKFNYPAARVKPARGTSISNVADLLEGVVKDANTGAQKEGIAVGMTGKEALDLLS"
     gene            complement(579..971)
                     /locus_tag="PKM87_11565"
     CDS             complement(579..971)
                     /locus_tag="PKM87_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HNQ30749.1"
                     /translation="MCPDNQGENPHRGRPRIRRKFGKRIAGRCFEPCNFMGGTVEEVI
                     LRQDELEILRLIDIEGLSQEEAAGVMGISRRTLWRDLQETRRKCAEALIFGKKITLDR
                     CAGNARGWCRREMNGETREDPPDTRDEM"
     gene            complement(958..1836)
                     /locus_tag="PKM87_11570"
     CDS             complement(958..1836)
                     /locus_tag="PKM87_11570"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ30750.1"
                     /translation="MLQVAVLSGKGGTGKTVVTAALADILPGRKGFADCDVEAANLAL
                     LLSPREKRRIPFYGMKKAEIREEACVSCGLCKAACRFHAINQDNGGAYRVDPIRCEGC
                     AVCRDICPERAVSLVEHQDGEIILADTEQGPLVYAELHPGSGNSGLLVHEVKKAAQKE
                     SEGRDLLLIDGPPGIGCPVISTLSGINAAVLVTEPGIAALHDLRRLVTVSSGFSLSHW
                     VVINRADLHPGYTKEIEQFCAEKGIPVIGKIPFDPAVIEAVRIGVPVTRRKGAASTEL
                     RIIGTRLAEGLSYVPG"
     gene            complement(1836..2684)
                     /locus_tag="PKM87_11575"
     CDS             complement(1836..2684)
                     /locus_tag="PKM87_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ30751.1"
                     /translation="MRLVVASGKGGTGKTLVATNLAAVLSSEREVVLADCDVEEPNAH
                     LFFPSEEKTLPVQTDVPLFDRSRCTQCGKCADFCQYGAIVVLKDRFMFFPEMCHGCGG
                     CEVVCPEGAITMGKRSIGHVAFSTPRNGLVLITGVLNEREIMSPHVIRETLGYASGHP
                     LVICDAPPGMACPVVETLEGADTCIFVTEPTPFGRHDLSLAAGVASVLGVPSGVVINR
                     SQGDDGIILDLCRERNLPVLMTIPFDRDIAETANSGQLVTDRIPGMREQFRDLFDRAC
                     DLRRGE"
     gene            complement(2692..3027)
                     /locus_tag="PKM87_11580"
     CDS             complement(2692..3027)
                     /locus_tag="PKM87_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNQ30752.1"
                     /translation="MMVCVTSKGPGSDDLAEERFGRSPYFLFRDSEGGEWSSVPNKFA
                     DAAGGVGPRAAQVLIDKGARVLITGNIGGNAHAVVQAAGIEIYLYRENGTAGDAFQKF
                     CAGQLKKFS"
     gene            complement(3086..3574)
                     /gene="tsaA"
                     /locus_tag="PKM87_11585"
     CDS             complement(3086..3574)
                     /gene="tsaA"
                     /locus_tag="PKM87_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HNQ30753.1"
                     /translation="MQYQIIGVIHSPFTDQKRTPIQSAFSSARGTVEVFPQYSEGLEG
                     LAEFSHLFLVYHFHQAKPGPLTETPFLDGERPRGIFSIRHYNRPNPIGLSIVEIEKLE
                     GNILFVKGIDVLDGTPLLDLKPYVARFDHRDGVRDGWIDAQQKREIKDESATPENLLG
                     TE"
BASE COUNT          750 a         1171 c         1000 g          856 t
ORIGIN      
        1 gcatccccca ccacgcgttc cctctgttca tccgcggcat tcgcaacccg ccagacgtcc
       61 cttcccctca ccttgaggag ggctgccgcc tcatccgggg tgagcctgtg ccccccccgg
      121 atgtcgccga gcagcacgtg gagggttctg cctgtgagga atgccggcgc taccgtgcta
      181 cctgttgaca tgggtcctct gacagagtgg tggcgtcatc aatataaaac cccacaccac
      241 ggcattcatc atcattgcac gagagatccc attgtatgca ggaagtgaaa gttacgctcc
      301 tgaactctga agggagaggg tttgtgatcc cactcggccc cgtcaacctg gtcggggttg
      361 tggcatcaaa agggctcgtg ggctgtggtg caatcgatgt tgccgcgctg gagaagttca
      421 actatcccgc cgcacgggta aaaccagcga ggggaacttc catctcgaac gttgccgacc
      481 tccttgaagg cgtggtgaag gatgccaaca caggtgcaca aaaagagggt atcgcggtgg
      541 gtatgacagg aaaagaggcg ctcgatctcc tctcctgatc acatttcatc tcttgtatcc
      601 gggggatcct cccttgtctc tccattcatt tccctgcggc accatcccct ggcattccca
      661 gcacacctgt ccagggtaat cttcttcccg aagatcaatg cctcggcgca tttcctcctt
      721 gtctcctgga gatcccgcca gagcgttctc cgggagattc ccatgacacc tgcagcctcc
      781 tcctgcgaga gcccctcaat gtcgatcaac cggagaatct cgagttcgtc ctggcggagg
      841 atcacctcct cgacagtccc gcccatgaag ttgcagggct cgaaacatct ccctgcaatc
      901 ctcttcccga atttcctgcg gatccttggc cgtcccctgt ggggattttc gccctggtta
      961 tccgggcaca tacgaaagcc cttctgcaag cctcgtcccg attatcctca attcagtgga
     1021 agcagcacct ttccgcctcg tgacgggcac ccctatcctc accgcctcta ttactgcggg
     1081 atcaaacggg atcttcccga tgacggggat gcccttctct gcacagaact gttcgatctc
     1141 ctttgtatac ccaggatgga ggtctgccct gttgatcacc acccagtggg agagcgagaa
     1201 gcctgacgag actgtgacaa gcctgcgcag gtcgtgcagg gcagcgatcc cgggttcggt
     1261 gacgagcacc gccgcgttga tcccgctcaa tgtcgagatc acggggcagc ctatccccgg
     1321 ggggccgtct atgagcagga ggtcgcggcc ttcggactct ttttgtgccg ccttcttcac
     1381 ctcgtggacc aggagcccgg aattcccgga acccgggtgc agctccgcat agacaagggg
     1441 accctgttcg gtgtctgcca gtatgatctc tccgtcctgg tgttcgacaa gtgagacagc
     1501 cctctcgggg catatgtccc tgcacacggc acacccctcg caccggatgg ggtctaccct
     1561 gtatgcacct ccgttgtcct ggttaatcgc atggaaacgg catgctgcct tgcagaggcc
     1621 gcaggacacg cacgcctctt cccttatctc ggccttcttc atgccataga acgggatcct
     1681 cctcttctcc cttggcgaga ggagaagcgc gaggtttgcg gcctccacgt cgcagtccgc
     1741 aaaacctttc ctgccgggga ggatatctgc cagtgcggca gtcaccaccg tcttccccgt
     1801 gcctcctttc cctgagagca ctgccacctg cagcatcact cccccctcct gagatcgcag
     1861 gccctgtcaa agaggtctct gaactgctct ctcatcccgg gtatcctgtc ggtcacgagt
     1921 tggccgctgt tcgccgtttc tgcaatatcc cggtcgaatg gaatggtcat gaggacgggg
     1981 aggttccgtt ccctgcagag gtcgagaatg atcccatcgt cgccctggct ccggttgatc
     2041 accactcccg agggaacacc cagtaccgaa gcgacgcccg cagcaaggga gaggtcatgg
     2101 cggccgaacg gcgtgggttc ggttacgaaa atgcaggtat ccgcaccttc gagcgtctcg
     2161 accaccgggc atgccatccc gggtggggca tcgcagatga ccaggggatg gccggacgca
     2221 tagccaagag tctccctgat gacatgggga ctcatgatct cacgctcgtt caggacgccc
     2281 gtgatgagga cgagaccatt gcgaggcgtg gagaatgcca catgaccgat gcttctcttc
     2341 cccatggtaa tggccccttc ggggcagacc acttcgcatc ccccgcatcc atggcacatt
     2401 tctgggaaga acatgaaccg gtccttgagg accacgatcg ctccgtactg acagaaatct
     2461 gcacattttc cacactgggt gcaccgtgaa cgatcgaaca gtgggacatc cgtctggaca
     2521 gggagggtct tctcctccga cggaaagaaa aggtgtgcgt tcggctcctc cacgtcgcag
     2581 tctgcgagga ccacctccct ttcagaggac agaaccgctg ccaggtttgt cgcgacgagg
     2641 gtcttgcctg tacccccctt tccgctggcc accaccagtc tcatgatatc cttatgagaa
     2701 tttcttgagc tggccggcgc aaaacttctg gaacgcgtct ccggcagttc cgttctccct
     2761 gtacaaataa atctctatac ctgccgcctg aaccaccgcg tgggcatttc ccccgatgtt
     2821 cccggtgatc agtacacggg cacccttgtc gatcagcacc tgggcggctc tcggtccgac
     2881 tccgcccgcc gcatcagcga acttgttcgg gacagagctc cactctcccc cttcactatc
     2941 cctgaagagg aaatatggcg atcttccaaa cctttcttcc gcgaggtcat cagaccctgg
     3001 gccttttgag gtgacacata ccatcatgat gatatatttt tactcatatg agcatatata
     3061 attggggatt gtacccctca attggctact ctgtccccag caggttctcc ggggtcgcac
     3121 tctcgtcttt gatctccctt ttttgctgtg catcgatcca accatcccgg accccgtcac
     3181 ggtggtcgaa ccgtgccacg tagggcttga gatcgaggag aggggtcccg tcaaggacgt
     3241 cgataccttt tacgaagaga atgtttcctt ctaacttctc gatttcaacg atcgacagcc
     3301 cgattggatt gggccggttg taatgcctga ttgagaagat ccccctcggc ctctctccat
     3361 caaggaacgg agtctccgtc aacgggccag gtttggcctg gtggaaatgg tagacgagaa
     3421 acaggtggga gaactccgcg agaccttcga ggccctcgga atattgaggg aagacttcca
     3481 ccgtccccct tgcactcgag aaggcgctct ggattggggt gcgcttctga tcggtgaacg
     3541 gggagtgaat gaccccgatt atctgatact gcactgtgca aatttctttc atcgacaggg
     3601 aattatcttg gtggaaaaac tatgaacatt gtgattttat caaataatcg tcttttccat
     3661 cagcatgtac tatccaacac ttaaagaaat ccggagcgtt aagtagtata ccaatgggat
     3721 atacccacct cttcgggccg gtctcgtccc gccgcctggg acgatctctt ggggtag
//
LOCUS       DAOQYR010000150         3764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_25190_length_3764_cov_10.363710, whole genome shotgun
            sequence.
ACCESSION   DAOQYR010000150 DAOQYR010000000
VERSION     DAOQYR010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25190_length_3764_cov_10.363710"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PKM87_11590"
     CDS             complement(<1..260)
                     /locus_tag="PKM87_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNQ30754.1"
                     /translation="MAFFEMAKTAILTTLKRPATRRYPAVPSKVTAISRGKVTINPSR
                     CISCGMCMKKCPSGAICVVKDEKTWSIDRLKCVVCNCCVEVCP"
     gene            complement(268..1347)
                     /locus_tag="PKM87_11595"
     CDS             complement(268..1347)
                     /locus_tag="PKM87_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNQ30755.1"
                     /translation="MSRRIVVPFGPQHPVLPEPIHLDLVLQDERVVEAIPSIGYIHRG
                     LETLVAKREYPEYVYIAERTCGICSFIHGMTYCQAVEQVMGLEVPERALYLRTIWSEY
                     SRMHSHLLWLGLFADAFGFENLFMASWRLREFVLDVLEETTGGRVIQGSCKVGGVRRD
                     IPADTLDRMVGDLADFERNYREIANVFLRDESVIGRTRGVGVLSRDDAYYLGAMGPTA
                     RGSGVAIDLRETGYAAYGKINFKPVVEEGGDCYARCAVRVKEIFSSIDIVRQAAARIP
                     EGEIEVKPKGNPDGECFSRAEQPRGELVHYVKGDGSRHLVRSRIRTPTLTNIPPLVKM
                     LAGCELADVPVIVLSIDPCIGCMER"
     gene            complement(1332..1712)
                     /locus_tag="PKM87_11600"
     CDS             complement(1332..1712)
                     /locus_tag="PKM87_11600"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNQ30756.1"
                     /translation="MTMEAQDTTLVEQDHLIGRVEEFKKKGYRLVQMSCTKVGEKYEI
                     NYSFDLHYTFQNLRIVVDGQTEVPSISGIYWGAFIYENEMHDLFGIRIVGMNIDFGGN
                     LFRTAKKNPFANPTFREERPCHGE"
     gene            complement(1709..2167)
                     /gene="nuoB"
                     /locus_tag="PKM87_11605"
     CDS             complement(1709..2167)
                     /gene="nuoB"
                     /locus_tag="PKM87_11605"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HNQ30757.1"
                     /translation="MTHLSKSPWILHYDASSCNGCDIEVLACLTPLYDVERFGIINTG
                     NPKHADMLVITGGINEQNRAVVKNLFDQMPDPKVVVAVGICACTGGVFRECYNIAGGV
                     DCIIPVDVYVPGCAARPESIIDGIVRALSVLEEKKAKLVKPERAGSGGER"
     gene            complement(2164..3030)
                     /locus_tag="PKM87_11610"
     CDS             complement(2164..3030)
                     /locus_tag="PKM87_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNQ30758.1"
                     /translation="MIMVEYALLFIVLAPFVGGLVAGVDRKLTARMQGRVGPPLLQPF
                     YDVGKLFQKEQIVVTPSQHFYILSYLVFIVASGALFFAGGDLLLVIFAFTLAHIFLVL
                     GAYSSYSPYSFIGAERELIQIIAYEPMIILAAVGMYMVTKSFFVWEIMASSLPLILYL
                     PGVFLGFLMVLTIKLRKSPFDLSTSHHAHQEIVKGVTTEFSGPMLARVEIAHWYENVF
                     LLGFIYLFFAWNPVYGIIAIIVAYLLEILIDNTFSRGTWKFTLKSAWIIAGMMGLVNL
                     GVLYYLSGVVPV"
     gene            complement(3027..>3764)
                     /locus_tag="PKM87_11615"
     CDS             complement(3027..>3764)
                     /locus_tag="PKM87_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNQ30759.1"
                     /translation="LFLCVGTVEHRIGSKDIEDMTGLIVRMPHVAVMMFIGIAGMFLA
                     PFGMLISKWAAIEAFIEAQFGLIFVAILAFGGSITVFFWSKWMGKIISVCPVATKVEN
                     RIPQEKWIALSLLTAMTVFICLAFPIISSHLIEPFVLSVYGQTARLSQDNITIMAMML
                     FLLMIMPFSLLWFRKEKKIVPPYMAGRPTTPDLHFLGSMGVQRQVTLGNYYLEEVFGE
                     KRLFMAGTALCFGLILLVGTLTAGVAL"
BASE COUNT          816 a         1100 c         1065 g          783 t
ORIGIN      
        1 gggcatacct ccacgcagca gttgcacacc acgcacttca ggcggtcgat actccaggtc
       61 ttctcgtcct tgaccacgca tatggctcct gacgggcatt ttttcatgca catgccgcat
      121 gagatgcagc gtgaaggatt aatcgtgacc ttaccccggg agatggcagt cactttcgac
      181 gggactgcgg gataccgcct ggttgccggc ctcttcaggg tggtaaggat tgcggttttt
      241 gccatttcaa agaatgccat cctcttctca cctctccatg cacccgatac acgggtcaat
      301 actcagcacg attaccggta catcagcaag ctcgcaccct gcaagcatct tcaccagggg
      361 cggaatgttc gtgagtgtcg gagtgcggat tcgggagcgc accaggtgcc ggcttccgtc
      421 acccttgacg tagtgcacga gttcccctcg gggctgctcg gcgcgtgaga agcactcgcc
      481 atccggattt cccttcggct tgacctcgat ctccccttcg gggatacggg cagcagcctg
      541 gcgcacgatg tcgatcgagg aaaagatctc cttcaccctc acggcacacc gcgcatagca
      601 gtctcccccc tcctccacca cgggtttgaa gttgatctta ccataggccg cgtatcctgt
      661 ctccctcagg tctatggcca caccactgcc ccgcgcggtg gggcccatcg caccgaggta
      721 gtacgcatca tcacgtgaga gcaccccgac tccgcgtgta cggccgatga cggattcatc
      781 gcgaaggaac acattggcga tctccctgta attgcgctcg aagtcagcaa ggtccccaac
      841 catacggtcg agggtatcag ccgggatgtc cctccttact cctcccacct tgcagctccc
      901 ctggataaca cgcccgccgg ttgtctcctc aaggacatcg aggacgaact cccgcagtct
      961 ccaagaggcc atgaaaaggt tctcaaaacc aaacgcatcg gcaaagagcc cgagccacag
     1021 gaggtgcgag tgcatcctgg aatactctga ccagatggtc ctgagataga gggcacgttc
     1081 gggcacttcg aggcccatta cctgttcgac tgcctggcaa taggtcatcc cgtggatgaa
     1141 gctgcagata ccgcaggtcc tctcggcgat atagacgtat tcgggatact cgcgttttgc
     1201 caccagggtc tcgaggcccc tgtggatgta tccgatcgac gggatcgcct ctaccaccct
     1261 ctcgtcctgt agtaccaggt caaggtggat gggctcgggg agtacggggt gctgcggccc
     1321 gaacgggacg actattctcc gtgacatggt ctctcctccc tgaaagtggg atttgcaaat
     1381 ggattctttt tcgcggttcg gaacaggttt ccgccaaagt cgatattcat ccccacgatc
     1441 cggatgccaa agaggtcatg catctcgttc tcgtagatga acgcccccca gtatatcccg
     1501 gaaatactgg ggacttctgt ctgcccgtct accacgatac gaagattctg gaacgtatag
     1561 tgtaaatcaa aagagtagtt gatctcgtat ttttccccga ccttggtgca gctcatctgt
     1621 actagccggt atcccttttt cttgaattct tccactctgc cgatcaggtg gtcctgctct
     1681 accagtgtgg tatcctgtgc ttccattgtc atctctcacc tccggaacca gcccgttcag
     1741 gttttaccag cttcgccttc ttctcctcga ggaccgagag tgcccgcacg attccgtcga
     1801 tgatggattc gggtcgtgcg gcgcatcccg ggacatacac gtccaccggg atgatgcagt
     1861 cgacaccacc cgcgatattg tagcactccc tgaacactcc gccggtgcag gcgcagatcc
     1921 cgactgcgac caccaccttc gggtcaggca tctggtcgaa gaggtttttt acaactgccc
     1981 tgttctgctc gttgattccc ccggtgataa ccagcatgtc ggcatgtttc gggttgccag
     2041 tgttgataat cccgaaccgc tccacgtcat agagaggggt gaggcaggcc agcacctcga
     2101 tgtcgcagcc attgcagctg gatgcatcat agtggaggat ccatggggac ttcgagaggt
     2161 gggtcatacc ggcaccaccc ccgaaaggta atagagcacc ccgagattga cgagccccat
     2221 cattcccgcg atgatccatg cgctcttcag ggtgaatttc caggtcccgc gggagaaggt
     2281 gttgtcgatc aggatctcga gcaggtaggc gacgatgatg gcgatgatgc cgtacaccgg
     2341 gttccatgca aagaagagat agatgaaccc aaggaggaag acgttctcgt accagtgggc
     2401 gatttccact ctcgcaagca tcgggcctga gaactcggtc gtcacccctt tcacgatctc
     2461 ctggtgcgcg tggtgcgagg ttgagaggtc gaagggagac ttcctcagct tgatggtcag
     2521 gaccatgagg aacccgagga agaccccggg gaggtagagg atcaggggaa gtgaactcgc
     2581 catgatctcc cagacaaaga agctcttggt caccatgtac atgcccacgg ctgcaaggat
     2641 gatcatcggc tcgtaggcga tgatctggat aagctccctc tcggcgccga tgaagctgta
     2701 aggcgagtat gacgagtagg cgccgagcac caggaatatg tgggcgagcg tgaatgcaaa
     2761 tatgaccagc aggagatcgc ccccggcgaa gaagagtgcc ccgctcgcca cgatgaagac
     2821 gaggtatgag aggatgtaga agtgctgaga tggggtcacc acgatctgct ccttctggaa
     2881 gagctttccg acatcgtaga atggctggag gagcggcggg cccacgcgtc cctgcatcct
     2941 ggcggtcagt ttgcgatcga cccctgccac cagcccgcca acgaaggggg ccagcacgat
     3001 aaacaggagg gcatactcca ccatgatcat agcgccaccc ccgcggtgag ggttcccaca
     3061 agcaggatga ggccgaaaca gagcgccgtc ccggccatga agagtctctt ctcaccgaag
     3121 acttcctcca gatagtaatt tccaagggtc acctggcgct ggactcccat ggagccgagg
     3181 aaatggaggt cgggcgtcgt gggcctcccc gccatgtagg gtggcacgat ttttttctcc
     3241 ttcctgaacc agagcaggga gaatggcatg atcatcagga ggaagagcat catggccatg
     3301 atggtgatgt tgtcctggga gagccgtgca gtctgtccat agaccgagag aacgaacggc
     3361 tcgatgagat gcgaggagat gattgggaac gcaaggcaga tgaacaccgt catcgcggtg
     3421 aggagcgaga gtgcgatcca tttctcctgc ggtatgcggt tctccacctt cgtggccacg
     3481 ggacagacag agatgatctt tcccatccac tttgaccaga agaagaccgt gatcgatccc
     3541 ccgaaagcaa ggatggccac gaagatgagg ccgaactggg cctcgatgaa ggcctcgatg
     3601 gcggcccact tggatatcag catcccaaac ggtgcaagga acatccctgc gatcccgatg
     3661 aacatcatca ctgccacgtg cggcatgcgc acgatcaggc cggtcatgtc ctcgatgtcc
     3721 ttgctgccga tgcgatgctc gactgtgccc acgcagagga agag
//
LOCUS       DAOQYR010000151         3736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_25483_length_3736_cov_8.251290, whole genome shotgun sequence.
ACCESSION   DAOQYR010000151 DAOQYR010000000
VERSION     DAOQYR010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25483_length_3736_cov_8.251290"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..1162
                     /locus_tag="PKM87_11620"
     CDS             296..1162
                     /locus_tag="PKM87_11620"
                     /EC_number="2.7.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate kinase"
                     /protein_id="HNQ30760.1"
                     /translation="MPSFATAFSPGHLSGYFRPVYGTDPAQTGSLGAGIVIQEGVTST
                     IWPSDKTEISVERLDPVSGCMVEIVPASPPIHYLLERMGVTGTVRTECCLPIGAGFGL
                     SAAALLSTATAASALFGLSLAPAECAALAHEAEIVHRTGLGDVAACMGGGRDCRKGPG
                     IRAEIERTFDIREPLCAVIFGPLPSPEVLGSPEAMARVIRAFPGRCPVDARDFFSLSR
                     RFAVESGLATPQVVEALSACDEKGVPATMTMLGNGVFAYGDEAFPLLTCLGEALVLHP
                     ADAGVHLLEVGG"
     gene            1159..1890
                     /locus_tag="PKM87_11625"
     CDS             1159..1890
                     /locus_tag="PKM87_11625"
                     /EC_number="6.3.2.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphopantoate--beta-alanine ligase"
                     /protein_id="HNQ30761.1"
                     /translation="MIPPEHPRYRSLFARERLARYAAEGIVSLEGLSAHGRGEAFDYL
                     LGEKTTASALLAEKTAAALLLTARDPVISVNGNTAGLAAELIAPLQEKSGAKVEVNLF
                     HRTEERIKKISRLLRDHGVRLEEGNAERLLPLSHDRALCQRGGIYQADVILVPLEDGD
                     RCQALREMGKTVIAIDLNPLSRTARSASLTIVDELTRALPNLTSFCASLPRHEAEALA
                     GSLDNTRFLADALEMMKEGLSHAMD"
     gene            1877..2890
                     /locus_tag="PKM87_11630"
     CDS             1877..2890
                     /locus_tag="PKM87_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ30762.1"
                     /translation="MLWIEKYRPRTFEEIIGQEKVVERLKHFASFRAVPHLMLAGPPG
                     TGKSASVECLARELYGDHAEENLSVVPTSDLFMLGKKFLECEERYAHLYRADESLLSN
                     FKRIIREYSSLRPLDAEFKLLAFEGASSLPREAQQALRRIMERSSRTCRFIYCTSHPS
                     AVIPAIASRCLPVFFSPIDDALVESHLHTICAAEGKDNCPVREDQVGLLVAAARGDLR
                     KAVMLLQVLAESGPDANLLSLAKTETGQVATAAFSAMQAGDVQAATRRLETLMIEYGL
                     SAREVLGELGNVVKRDFNDPHIACVIAETDWILGHSQNEYLQLNALVAKIARETGDAR
                     RQG"
     gene            complement(3104..>3736)
                     /locus_tag="PKM87_11635"
     CDS             complement(3104..>3736)
                     /locus_tag="PKM87_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNQ30763.1"
                     /translation="DVILMTEGAGGGTIATTALYSGYPEVVEQTINLHFLQACEALLA
                     SPALQKVHAMTDVTNGGLRGDAHELAATAGCRVVIEEENVKGLVQEEVGALLERLSID
                     PLGVSLDALLITAPPDAAHPIMEVIRGAGVRIREIGAVEKGKPGAYLLTSKGLSDFTP
                     RFREAAYTPVKKVVDRSGGDFAEMKRAVEAAASAAIEKKERMVARLKKGR"
BASE COUNT          728 a         1181 c         1083 g          744 t
ORIGIN      
        1 ccctgcccac aggctatcgc gtatcagggg gaggtccgga gggggattct ccccctccgg
       61 tcagggaaaa aaccgcactc atcacacgat gacgttctcg gggtccaggg gcggaagcct
      121 tcgcggagtt ttccctcccc attggaggcg tccttggcaa ttgagccctt aaaacccttt
      181 tttccgctgc tctgataaac ccgtcaattg cgtgtttcat tcctggcggg tcactttctt
      241 aagcttttaa tacgaaccaa ttgcaggaaa aaactctcct gtccctgtga tcgccatgcc
      301 ctcctttgcc actgcattca gtcccggcca cctctcgggg tacttcaggc ctgtctacgg
      361 gaccgacccg gcgcaaacgg ggagtcttgg cgcagggatt gtgatacaag agggagtaac
      421 ctccaccatc tggccctccg acaagacaga aatatccgtg gaacgcctgg accctgtttc
      481 gggatgtatg gtcgaaatag ttccggcttc acctcccatc cattacctcc ttgagcggat
      541 gggtgtcacc ggaacggtga gaactgaatg ctgcctcccc atcggtgccg gtttcggcct
      601 ctccgcagca gccctcctct ccaccgcaac tgcagccagt gcactctttg gcctctcgct
      661 cgccccggcg gaatgcgcgg cattggccca cgaggcagag atcgtccaca ggaccggact
      721 tggagatgtt gcggcctgca tggggggcgg ccgggactgc aggaagggtc cagggatcag
      781 ggccgagatc gagcgcacct ttgacatccg cgaacccctc tgtgccgtga tcttcggccc
      841 gctcccatcc ccggaagtac ttggctcccc ggaagccatg gccagggtca tcagggcctt
      901 tccgggccgc tgcccggtgg acgcgcggga cttcttcagc ctctccaggc ggtttgcagt
      961 ggagagcggt cttgccacgc cgcaggtggt cgaggcactc tccgcctgtg acgaaaaggg
     1021 ggtgcctgcg accatgacaa tgctcggaaa cggggtcttc gcatacggag atgaggcgtt
     1081 ccccctgctc acatgcctgg gcgaggcgtt ggtgcttcac cctgccgatg cgggagtgca
     1141 tctcctggag gtggggggat gataccgccg gaacaccccc ggtaccgatc gctcttcgca
     1201 agggagcggc ttgcacgtta cgctgcggag ggaatcgtct cgctcgaagg actttctgcc
     1261 cacggacgcg gcgaggcgtt cgactacctc ctcggggaga agaccactgc aagcgcgctt
     1321 ctcgcagaaa agacggccgc agcactcctc ctcaccgcaa gggaccctgt catctctgtg
     1381 aacggcaaca ccgcaggcct ggctgcagaa ctcatcgctc ctctgcagga gaagtcaggc
     1441 gcaaaagtgg aggtcaacct cttccaccgg accgaggaga gaataaagaa gataagccgc
     1501 ctgcttcgcg accatggtgt ccgactggag gaaggaaatg ccgagaggct cctcccgctc
     1561 tcccacgacc gggcattgtg ccagagaggg gggatctacc aggcagatgt catccttgtg
     1621 ccgctcgaag acggcgatcg ttgccaggcg cttcgggaaa tgggcaagac agtgatcgcg
     1681 atagacctca acccactttc gaggaccgcc cggagtgcaa gcctcacgat tgtcgacgaa
     1741 ctcacaaggg ccctccccaa cctcacctcg ttctgcgcat cgctcccaag gcacgaggcc
     1801 gaggcacttg cagggtcgct tgacaatacc aggttcctgg cggatgccct cgaaatgatg
     1861 aaggagggac tctcccatgc tatggattga gaagtaccgc ccccgcacgt ttgaagagat
     1921 catcgggcag gagaaggtgg tagaacggct gaagcatttt gcctcatttc gggcagtacc
     1981 tcacctgatg ctcgccggtc cccccggcac aggaaagagc gcgagcgtcg agtgccttgc
     2041 gcgggaactg tacggggacc atgcagagga gaacctcagc gtcgttccca cgagcgacct
     2101 cttcatgctc gggaaaaaat tcctggaatg cgaagagcgg tatgcacacc tttaccgcgc
     2161 agacgaaagc ctgctctcga acttcaagcg catcatccgg gagtattcat cgcttcgtcc
     2221 cctggatgcc gagttcaagc tgctggcatt cgagggtgcc tcatctcttc cccgcgaagc
     2281 acagcaggcg ctgcgtcgga tcatggagcg gtcttcgcgg acgtgcaggt tcatctactg
     2341 tacaagccat ccctcggcag tcatccctgc catcgcgtcc cgttgcctgc cggtattctt
     2401 ctctcctatc gatgacgccc tggtagaatc ccacctgcat accatatgcg ctgcggaggg
     2461 caaagacaac tgccccgtcc gggaagacca ggtcggcctc ctggttgccg ccgcacgagg
     2521 ggatctccgg aaggctgtca tgctcctcca ggtgcttgcc gagtcgggtc cggacgcaaa
     2581 cctcctctcc ctcgcaaaga ccgagactgg ccaggtggcg actgccgcgt tctccgcgat
     2641 gcaggcaggt gacgtccagg cagccacccg gaggctggag acgcttatga tcgagtacgg
     2701 gctctccgcg agggaggtgc tcggcgagct tggcaacgtc gtgaagcgcg acttcaacga
     2761 cccgcatatc gcctgtgtaa tcgccgaaac cgactggatc ctggggcaca gccagaacga
     2821 gtatctccag ctcaatgcac tggtggcaaa gattgccagg gaaacggggg atgcccggcg
     2881 tcaggggtaa agtcagtcaa gtacgtggca aggcagcatg ggttgagttc ctgtgtcacc
     2941 cgatgaaaaa aaggttcttt tcccatttgc acgctattcc aagacattca tcgaggaaac
     3001 tcccaattct cctctcatcc actctcatag agaagaataa aacctgcctt gaaggatagg
     3061 gattgccagg aaacccggca ttcctcctga tatgatcgac cacctaacgt cccttcttca
     3121 ggcgggcaac cattcgttcc ttcttctcta tcgctgcgct tgcggcggcc tcgaccgcac
     3181 gcttcatctc ggcaaagtct cccccgctcc tgtccaccac tttctttacc ggcgtgtagg
     3241 cggcctcccg gaatctgggg gtgaaatcag aaagcccctt gctggtgagg aggtatgcac
     3301 cgggtttgcc tttttccacg gccccgatct cccggatcct cacccctgca cccctgatca
     3361 cctccatgat cggatgtgcg gcatcaggtg gcgcggtgat cagaagcgca tcgagggaga
     3421 cccccagggg atcgatggag aggcgctcga gaagcgctcc gacctcttcc tgcacgaggc
     3481 ccttcacatt ctcttcctcg attacgaccc tgcacccggc tgtcgccgcc agttcgtgcg
     3541 cgtcgccgcg aagacccccg ttggtgacgt ccgtcatcgc gtggaccttc tgcagcgctg
     3601 ggcttgccag gagtgcctcg caggcctgga ggaagtggag gttgatggtc tgctccacga
     3661 cttcgggata ccccgagtag agtgccgtgg tggcgatcgt ccctcctcct gcaccctccg
     3721 tcatcaggat cacgtc
//
LOCUS       DAOQYR010000152         3592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_26960_length_3592_cov_7.417303, whole genome shotgun sequence.
ACCESSION   DAOQYR010000152 DAOQYR010000000
VERSION     DAOQYR010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26960_length_3592_cov_7.417303"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(470..1012)
                     /locus_tag="PKM87_11640"
     CDS             complement(470..1012)
                     /locus_tag="PKM87_11640"
                     /inference="COORDINATES: protein motif:HMM:NF015317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HNQ30764.1"
                     /translation="MITVEEKGNTPESNTTVVNECGYPETGKVMLVRRISRLVFLLAV
                     LGTGILAVALFIYGFIVTLASLAHAVTQPSMELEAMKEVMAISIEIIDLFLVATVFYI
                     ISLGFYELFISKAPLPGWLKICDLDDLKDKLLGLVVIALAVLFLGEALTWTGAADILG
                     YGLGTAATIIAISLYFWAKH"
     gene            complement(1185..1667)
                     /locus_tag="PKM87_11645"
     CDS             complement(1185..1667)
                     /locus_tag="PKM87_11645"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNQ30765.1"
                     /translation="MAYFCQQCGECCSVMGQVFSIVRQLDEFRYILRNEYTGDTREVE
                     VAPRLRRLFLESGVPAHWQDPCPFLRVDKAQGLAFCTVHLTRPDVCREYQCWRVLVLD
                     KEGRRVARVMEKRYLCLEDESLRGKWEEFRESLDHTDDSEWDEAVIRFFRGLGFCVLV
                     "
     gene            1751..1969
                     /locus_tag="PKM87_11650"
     CDS             1751..1969
                     /locus_tag="PKM87_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNQ30766.1"
                     /translation="MKRLVRMKEGKLLGGVCGGIGNYFEIDPVIIRILWVVLTLLSVG
                     IGVIVYIAAWLLMPEEGEGDPGTDTQSP"
     gene            complement(2082..2492)
                     /gene="tsaA"
                     /locus_tag="PKM87_11655"
     CDS             complement(2082..2492)
                     /gene="tsaA"
                     /locus_tag="PKM87_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HNQ30767.1"
                     /translation="MKIELHPIGYVRSPYRERRDAPRQGRLSDAVSEIVIDPAYEPGL
                     FRVAEKTHLFILCWFDRADRERLRVMPPKNPIEHGVFATRSPDRPNPVSLSVVDLLRV
                     EGCVLSVRGLDALDGTPVIDIKPFARDIDVPGVD"
     gene            complement(2489..3139)
                     /locus_tag="PKM87_11660"
     CDS             complement(2489..3139)
                     /locus_tag="PKM87_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HNQ30768.1"
                     /translation="MCENQEGTHFHSHYPSFEECVAFHGHACPGLATGYRVATAAMRT
                     LRVARPYDEELVCVAETNACGVDAIQMVTGCTAGKGNLVIHDYGKHAFSFFSRESGRA
                     IRVLVSRSDMPERSAMDGLRKKVFSGSATAKEEAEFRSLMQAATDHILLVPEDALLHI
                     REIPSQPPGKARIYATVTCACCGEPVADAKTRVVDGSRVCIPCAESRVQGGERRSP"
     gene            complement(3171..>3592)
                     /locus_tag="PKM87_11665"
     CDS             complement(3171..>3592)
                     /locus_tag="PKM87_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ30769.1"
                     /translation="RRLQMDHLRLSYIDEMSGGELQKIAIARAIVQEPKVLLLDEPTS
                     NLDLKNQVNILSIIARIVREHHIAAVMTMHDLNQALRFADRFIFLKGGRVYSHGGHDM
                     VSSAVIEEVYGLPVVIGELGGVRCVVPAGQACEVLND"
BASE COUNT          749 a         1040 c         1099 g          704 t
ORIGIN      
        1 gatacgggga gatcggcgtc tgggcaggga tcatcctcgg caacatcatc gggagcggtg
       61 tcgcctactt ctgggcccgg gcatatgtcg tccggctggt ggcagtcgtg caggagtgac
      121 ttctccctca tgggaagtcc ccgggaccac tttttcccga taaaaatggc tgcggcattt
      181 tcatgcttca tgctcgatcg tccagcggtc attggtggtt actatgaaaa tggctgcgcc
      241 attttcataa atgcgtatgc gtgtgtctca aagagaatca tgtagttttt ttcgtatacc
      301 ttacccgggg agattcgggc actcggtgca cgacagggta tcaaaaagag agggggtctg
      361 acaactgaat ggctccctga aaaaaattgc ctgcgcgatt cctttcccgt atacgtgccg
      421 aaaggctgaa aatcgcgagt gcgatctcca gggaaaaatg atgattccgt cagtgctttg
      481 cccagaagta cagcgatatc gcgatgatcg ttgcggcagt gccaagacca tacccgagga
      541 tgtcggcagc cccggtccac gtcagcgcct cgccgaggaa gaggactgca agtgcgatca
      601 ctaccaggcc gagaagcttg tctttcaggt catcgaggtc acagatcttg agccatccgg
      661 ggagcggtgc cttcgaaatg aagagctcat agaacccaag cgatatgatg tagaagaccg
      721 tcgccaccag gaagaggtcg ataatctcga tggagatcgc catcacttcc ttcatggcct
      781 cgagttccat ggagggttga gtgactgcat gggcaagact cgcgagagtg acgataaaac
      841 catagataaa aagcgcgaca gcaagaatgc cagtgccaag aaccgcgaga aggaatacca
      901 ggcggcttat ccggcggacc agcatgacct tccctgtttc agggtacccg cattcattta
      961 caacagttgt gttcgattca ggagtatttc ccttctcttc cactgtgatc acttctatat
     1021 tcccttgaat aagtacatct cacctatgaa ggtacataaa gcaagtgggc atgcgggaga
     1081 atcatggcgc tccgtaatac acgaacccta cggcgttttc tgcgggaact gccgaggcgc
     1141 accactcccg ccggcgaaaa gcggctgaac cctccccctc ccgctcatac aaggacgcag
     1201 aaccctagac cccggaagaa acggatgaca gcctcgtccc attcgctgtc gtcagtgtga
     1261 tcgagggact cccggaactc ctcccacttc ccgcggagac tctcgtcctc gaggcagaga
     1321 taccgcttct ccatgaccct ggcaaccctt cgcccctctt tgtcaaggac aaggacccgc
     1381 cagcactggt actcccggca gacatcgggc cgcgtcaggt gcacggtgca gaatgcaaga
     1441 ccctgggcct tatccaccct gaggaacggg caggggtcct gccagtgtgc ggggaccccg
     1501 gactccagga agagccggcg aagccggggg gccacctcta cttcccgggt gtcgccggta
     1561 tactcgtttc ggagaatgta gcggaactcg tcgagttgcc ggacgatgga gaagacctgc
     1621 cccatcacgc tgcagcattc cccgcattgc tggcagaagt aggccatgca ggggtactgt
     1681 tcgacccgga ggaatatgca ggtttgggaa taaaggaagg tttttccctc ccaagggcgg
     1741 tgtattctcc atgaaaagac tcgtgcggat gaaggaaggg aagctgctcg ggggagtctg
     1801 cgggggaata ggaaactact tcgagataga cccggtcatc atccggatcc tctgggtggt
     1861 gctcaccctc ctctcggtcg ggatcggcgt gatcgtgtac atcgcagcct ggctcctgat
     1921 gcccgaggag ggggagggtg atccgggcac ggacacgcag tccccgtgaa tacccgggat
     1981 tgcacatgga ccgggagcct cctgagggaa ggcgttcccg gcgaaaaacc acctcttttt
     2041 cctggcaaag ccctctaaaa aatggcgggc ggggattgta ttcagtccac gccggggaca
     2101 tcgatatccc ttgcaaacgg cttgatgtcg atgaccggcg tgccgtcgag ggcgtccagc
     2161 ccgcggacgg aaaggacgca gccttccacc ctgaggaggt cgacgaccga gagcgagacg
     2221 gggttcggcc tgtccgggga ccgggtggca aacaccccgt gctcgatcgg gtttttcggc
     2281 ggcattaccc ggagcctctc ccggtccgca cggtcgaacc agcagaggat aaagaggtgg
     2341 gtcttctctg cgacccgaaa gagccctggc tcgtatgcag ggtcgatcac gatctccgag
     2401 accgcgtccg agagcctccc ctgccgggga gcatcccgcc tctccctgta gggggagcga
     2461 acatacccga tgggatggag ctcgatcttc atggagagcg gcgctcccct ccctgcacac
     2521 gggattcggc gcaggggatg cagacccgcg agccgtcgac cacccgcgtc tttgcatctg
     2581 cgaccggctc cccgcagcag gcgcaggtga cggtcgcata gatccgggct ttccctggcg
     2641 gttgggaggg gatctcgcgg atatgcagga gggcgtcctc tggcactaag aggatgtggt
     2701 cagttgccgc ctgcatcagc gaccggaact cagcctcctc cttcgcggtt gccgatcccg
     2761 agaagacctt cttccggagt ccgtccatcg cggaccgctc cggcatgtcg gaccgggaga
     2821 caaggacgcg gatcgcacgc ccgctctcgc gggagaagaa ggagaacgcg tgcttgccat
     2881 aatcgtggat gacgaggttg cccttccctg cagtgcagcc ggtcaccatc tggatcgcgt
     2941 ccacaccgca ggcatttgtc tctgcgacac agacgagctc ctcgtcataa ggccttgcga
     3001 cccgaagcgt cctcatcgcg gcggtcgcaa cccggtaccc ggtcgcaagc cccgggcagg
     3061 catggccgtg aaacgcgacg cattcttcga atgatgggta atgtgagtgg aaatgggttc
     3121 cttcctggtt ttcgcacatg gatatctacc tgtatggtgg ggataggaca tcaatcattc
     3181 aggacttcgc acgcctggcc tgcagggacc acgcagcgga ctccgccgag ctcgccgatg
     3241 accaccggga gcccgtagac ctcctcgatc acggcagaac tgaccatgtc gtggccgccg
     3301 tgcgagtaga cccggccgcc tttgaggaag atgaaccggt cggcgaaccg gagcgcctgg
     3361 ttgaggtcat gcatcgtcat cacggccgcg atgtgatgct cgcggacgat ccgggcgatg
     3421 atggagagaa tattgacctg gttcttcagg tcgagattgc tcgtaggttc gtcgaggagg
     3481 aggacctttg gctcctggac gatggcgcgg gcgatggcga tcttctggag ctcgccgccg
     3541 ctcatctcgt cgatgtagga gagccggagg tggtccatct ggagccgcct ga
//
LOCUS       DAOQYR010000153         3430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_28792_length_3430_cov_11.573630, whole genome shotgun
            sequence.
ACCESSION   DAOQYR010000153 DAOQYR010000000
VERSION     DAOQYR010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28792_length_3430_cov_11.573630"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(536..988)
                     /locus_tag="PKM87_11670"
     CDS             complement(536..988)
                     /locus_tag="PKM87_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNQ30770.1"
                     /translation="MNEPPRNDYFKDQICFVTGANSGIGFALSEEHLKRGATVCLAGR
                     DPKKVSDAAGHLSAFEDRVHPLQVDVTSQTQVRKAIEQTAAPFILDRVAERKGIIIVP
                     EGFHDQLWRGYVHGDERAEEMLAAHAHNRRVALEEGDPAAAAAAPMHR"
     gene            complement(985..1746)
                     /locus_tag="PKM87_11675"
     CDS             complement(985..1746)
                     /locus_tag="PKM87_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HNQ30771.1"
                     /translation="MPAHFGGNPFDTSYRARQKATMLAMTFETERAELERYIPEELEL
                     LAPEVQVSFNQLTEINWLAGGHYNLLNVSAPVRFNGKKDHVDSAYPLVIWENRTAPIL
                     TGREQTGVPKIFADIEDLHVFRPHYATSISYEGNTFLSMQFEAERQMTGNELEQAKRQ
                     FRQLDLIGWRYIPKVGVPGADLSQFIYFPQWIEVENVSEGKGTLEWTELSVMQNPRQY
                     QIIKALAALPVKKMPRSAMVEGEVVLDTSQARVLA"
     gene            1959..2927
                     /locus_tag="PKM87_11680"
     CDS             1959..2927
                     /locus_tag="PKM87_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNQ30772.1"
                     /translation="MRIKIGYFAVTDAMTPAGCLRQSIEAEKIGFDSLWVEDHLIAMP
                     GGKECSFAWTVMSSALQATKKVPFITGVTAPIMRYHPAIVAQAFATMAAMYPGRVGLG
                     VGTGEPPNEMAVYDGEWPSNNARLEMLEEALTVMNRLWTSDAPISHAGKYYTLKNAVL
                     LTRPKEKVPMYVSAIGPRAADLAGRLGDHLITLANNPQYIRDELVPAFEAGARASGRD
                     PKPMERVGHFAYVYDPEQVVTLENLQQDAGTIQGGAMAKAFSSEFISIFHTPEDFIRK
                     IEAMERMGYTHLAIADQRMGIVKGTLPSLDEAAGLRIWKDVLPHVR"
     gene            2989..>3430
                     /locus_tag="PKM87_11685"
     CDS             2989..>3430
                     /locus_tag="PKM87_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNQ30773.1"
                     /translation="MKTKIGYYAIGDAWTPAECLQQAVAAERIGFDTIWVEDHLIAMP
                     GGIECSFAWTVMSSALQATKKVPFITGVTAPIMRYHPAIVAQAFATMGAMYPGRVGLG
                     VGTGEPPNEMAVYGGEWPSNNTRLEMLEEALMVMNRLWTSDTPIS"
BASE COUNT          757 a          925 c          998 g          750 t
ORIGIN      
        1 gcatcaacac gattgcaccc gtcctcctcc cgaacctgct cgggatggtg ctctacgcct
       61 tcgtcctgcc tctcctcgtt attatcatgg ggcttctctc gctggtcacg gtctcaatca
      121 cccagaagct ccagcagggt gctgcaaccg cttcgtgaag agagatcctc tctcttttcc
      181 tgtcacgatg ggcagaccag ttcggtgaaa tcaggggagt tgatcgggag gtctcatggt
      241 ttttggaagg gaacagggag ttgcccctgc ctccggccag gttcccgtca gtgcatccat
      301 gaccacgagg tcatatccgc tcgcaggata gccacgcacc gaggcccgct gattcgaaga
      361 tctgaatgaa agaaacaacc tgatttcctt tgtgacacaa gcagaacgaa tgaaaatgca
      421 agaagcatgg tcatttacaa caacctgatt cccgtcgtta tataaggaga aagactgaaa
      481 atacgggaat catggtcatg atacaagggg aggagggact gatgatcctg gaattttacc
      541 ggtgcattgg tgccgctgcc gccgctgcgg gatcgccttc ttccagtgcg accctgcggt
      601 tatgggcatg ggctgcaagc atctcctctg ctctttcatc tccatgcaca tagcctctcc
      661 agagttgatc atgaaatcct tcggggacga tgatgatccc cttccgttcg gcaaccctgt
      721 caaggatgaa gggtgcagca gtctgttcga tcgccttcct cacctgcgtc tggctggtaa
      781 cgtccacctg cagggggtgc accctgtctt caaacgccga gaggtgtcca gcggcatccg
      841 atactttttt tgggtcgcgg cctgccaggc agacggtggc tcccctcttc aggtgttctt
      901 cactcagagc aaaaccaatc ccggagtttg ccccggtcac gaaacagatc tggtctttga
      961 aatagtcgtt tcgtggtggt tcgttcatgc gagtacccgt gcctgggagg tgtcaaggac
     1021 gacctccccc tcgaccatgg cggaccgggg cattttcttc acggggagtg ctgcgagggc
     1081 tttgatgatc tggtattgcc gggggttctg catcacagag agctcggtcc actccagggt
     1141 ccccttccct tcagagacat tctccacctc aatccactgg gggaaataga tgaactgcga
     1201 gaggtctgct cccgggacac cgacctttgg aatataccgc cagccgatga gatccagctg
     1261 cctgaactgg cgtttcgcct gttccagctc gtttccggtc atctgacgtt ccgcttcgaa
     1321 ctgcatcgag aggaaggtat tcccttcgta acttatcgat gtggcataat gaggccggaa
     1381 gacatgcagg tcctcgatgt ccgcaaagat cttcggcacg ccggtctgtt cccgcccggt
     1441 gaggatgggg gcggtcctgt tctcccagat aacgagaggg tatgcggaat caacatggtc
     1501 tttttttcca ttgaaccgga cgggtgcgga tacgttcagc aggttgtaat gcccgccggc
     1561 aagccagttg atctcggtca actggttgaa ggagacctgc acttcgggtg ccaggagttc
     1621 gagttcttcc gggatgtagc gttcgagttc ggcacgctcg gtttcaaagg tcatcgccag
     1681 catcgttgcc ttctgacgtg cccggtagga cgtatcgaac ggattgcccc cgaaatgggc
     1741 aggcatcaga taggtggagt ctttttgcag tcgaaacatg gtgatcgtcc aactattcct
     1801 gctgtatgga taaaagcgta tttaatttca tgagtgcccg gtaattctgc ttgatttttt
     1861 caccgtgaga aacggaattg ttttatgagt ttgtggtgta tgcatcctat gatcacctct
     1921 tcccactcga aggagaagag ggaagctgag gagagagaat gagaatcaaa atcggatatt
     1981 ttgcagtaac cgatgccatg accccggccg ggtgtttacg gcagtcaata gaggctgaga
     2041 agatcggatt tgactcgttg tgggtcgaag atcacctgat cgcgatgcca ggggggaagg
     2101 aatgcagctt cgcctggacg gttatgagct cggcgctcca ggcgacaaaa aaggtcccct
     2161 tcatcactgg cgtcaccgct cctatcatgc ggtaccaccc ggccatcgtt gcacaggcct
     2221 tcgccaccat ggcagcgatg tacccgggac gcgtgggtct tggcgtgggc acgggtgagc
     2281 cgccaaacga gatggcggtc tacgacggcg aatggccctc gaataacgcc cggctcgaga
     2341 tgctcgagga agccctcacg gtaatgaacc ggctgtggac gagcgatgcc cctatcagcc
     2401 atgccgggaa gtattacacc ctcaagaacg cggtcctgct cacgaggccg aaggagaagg
     2461 tccccatgta tgtcagcgca atcggaccac gtgcagcaga tctcgcggga cgcctcggtg
     2521 accacctgat cacgcttgca aacaaccccc agtatatcag ggatgagcta gtgccggcct
     2581 tcgaggctgg tgccagggca tcgggcaggg acccaaagcc catggaacgc gtcgggcatt
     2641 tcgcttacgt ctacgacccg gagcaggtag tcacgcttga aaacctccag caggacgctg
     2701 gaaccatcca gggaggcgcc atggcaaagg ccttttcgag cgaattcata tcgatcttcc
     2761 atactccgga agatttcatc aggaagatcg aagcaatgga gcggatgggg tatacgcatc
     2821 ttgcgatcgc cgaccagaga atggggatcg tgaaggggac ccttccctcc ctggacgaag
     2881 ctgcgggact caggatatgg aaggacgtcc ttccgcacgt cagataagac gtactggcgt
     2941 acgatgcgaa acgaacgttg gatgaagaca atgggatgag ggaacaggat gaaaacaaaa
     3001 attgggtatt atgcgatcgg cgatgcctgg acaccagccg agtgtctaca gcaggcagta
     3061 gcagccgaaa gaatcggatt tgacacgata tgggttgaag atcacctgat agcgatgcct
     3121 ggggggatcg aatgcagctt cgcctggacc gttatgagtt cagcgctcca ggcgacgaag
     3181 aaggtcccct tcatcaccgg cgtcaccgcc cccatcatgc ggtaccaccc ggcgatcgtt
     3241 gcacaggcgt tcgctaccat gggagcgatg tatccgggac gcgtcggact cggcgtgggt
     3301 acgggcgaac cgccaaacga gatggcggtc tacggcggcg agtggccgtc caacaatacc
     3361 cggcttgaga tgcttgaaga ggcactcatg gtcatgaacc ggctgtggac gagtgatacg
     3421 cccatcagct
//
LOCUS       DAOQYR010000154         3417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_28978_length_3417_cov_3.954491, whole genome shotgun sequence.
ACCESSION   DAOQYR010000154 DAOQYR010000000
VERSION     DAOQYR010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28978_length_3417_cov_3.954491"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PKM87_11690"
     CDS             <1..245
                     /locus_tag="PKM87_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNQ30774.1"
                     /translation="YQACADPVRLKILSLLMVQPLCVCVIKAVVKIADSKLSYHLNIL
                     KKTGMIEGEQQGQYIIYQITDKARTFLDQERVRFQR"
     gene            complement(236..658)
                     /locus_tag="PKM87_11695"
     CDS             complement(236..658)
                     /locus_tag="PKM87_11695"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNQ30775.1"
                     /translation="MTPGRHGRRSPGLSPYATIFKALSDETRLRIYLLLGNGELCVCQ
                     IQVALALPQTKISRHLTVLRNAGLVTARREGLWMYYQRAKPDNRSLRAFAEGIVSLLE
                     VDPNLVASVVADQRYAELPPEEITRLTKGGQAGDSLQR"
     gene            complement(660..3260)
                     /locus_tag="PKM87_11700"
     CDS             complement(660..3260)
                     /locus_tag="PKM87_11700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNQ30776.1"
                     /translation="MTASLVRSEGRRSTWWREPQQLALYGCGLITLVAFIAGYFGASL
                     ILVNGLYLLAVLVGIFYPARKALIALRNLTPTIHLLMLIGSVGAMLLGLWGEAAVLIF
                     VYSLGDVLESYAVDKARGAIRSLMALVPKEALVRRNGREIICAVDTIGVGEVVRVRPG
                     ERIPVDGTVISGTSFVDQAAVTGEPIAVNRQSGDEVFAGTINQNGSLEVRVTKPVSET
                     MLSRIIMSVEEAQAKRTSYQRFADSFGKWYTPAMFVLGVMVAIVPPLFFGAPWYPFIY
                     RGLVVFVVSCSCGLALSVPVAVVGAMANAARHGTVFKGGTYLEVTDKVRVIAFDKTGT
                     LTIGRPEVTDILAFGDVTPDEVLELAGCIEVQSGHPLAAAIVRKARESGPLPDQLVEG
                     FHETAGRGVAANVGERSCVLGSPRSLSEQGISIDEAGETIARFEDEGRTVVLVGRDGY
                     LIGLIAIADEVRPGAVDAVQRLSRAGVRTVMLTGDNDRSARAIAHRVGVDEYRAQLLP
                     TDKVDVIRQLKEEHGTVAMVGDGINDAPAMAVANVGIAMGAAGTDIAIEAGDIVLMSD
                     DLGKIAFIRELSHRTVTTIRQNIAVSLINVAFMVIAALLGYLGLVTGLLLNEASAVFV
                     ILNALRLLTWRSSSDHGASPGEGMPLPAITTPGEAAAPNPAAGCCCSSGAEPVVAVPS
                     GNHSVAPPCGNETGGAVALSSAVPETAGSGCGCTVPIIPASGNISTCCCGENSQDRTL
                     IPGNCCSSDTEEVPVQEAASTGCCSREPAAATNYPGLLAATFQIQGLGCACEGQIVEK
                     RLKGKEGITFFSLNPITNQMKLIYNPSLLSRREIEIEVKKAGVTPVFVKSEAVTASTG
                     STLPKNGHGG"
BASE COUNT          726 a         1045 c          956 g          690 t
ORIGIN      
        1 ggtaccaggc gtgtgccgat ccggtccgat tgaagatcct ctcccttctc atggtccaac
       61 cgctctgtgt ctgcgtgatc aaagcagtgg tcaagatcgc cgattccaaa ttgtcttacc
      121 atctcaatat cctgaagaaa accgggatga tcgaagggga acagcagggg cagtatatca
      181 tttaccagat caccgacaag gcacgcacct ttctcgacca ggagagggtc cggtttcaac
      241 gctgaaggga atccccagcc tgcccgcctt ttgtcaaacg ggtaatctcc tcgggaggca
      301 gctcggcata tcgctgatcg gccaccaccg atgcaaccag gttgggatcc acttcgagaa
      361 gactgacaat cccctcagcg aatgcacgaa gtgaacgatt atccggtttc gccctctggt
      421 aatacatcca gagaccctcg cggcgggcag tcaccagccc tgcattccgg agcacggtga
      481 ggtggcggga gatttttgtc tgtggcagtg ccaacgcgac ctggatctgg cagacacaga
      541 gctcgccatt ccccagaagg agatagatcc ggagccttgt ctcgtcagag agcgccttga
      601 atatcgtggc atagggggag aggccgggag atcggcgacc atggcgaccg ggggtcatcc
      661 tatcctccgt gaccgttctt gggaagggtg ctcccggtcg atgcggtaac tgcctctgac
      721 ttcacgaata ccggcgttac tccggctttt ttcacctcaa tttcgatctc cctccgtgag
      781 aggagcgagg ggttatagat gagtttcatc tgattcgtaa tgggattcaa agaaaaaaag
      841 gtgatccctt cctttccttt caaccgcttc tcgacaatct gtccttcgca ggcgcatccc
      901 agtccctgga tttggaaggt tgcggccagg agtccgggat agttcgtggc agctgccggt
      961 tccctagagc agcacccggt gctcgcagcc tcctggaccg gaacctcttc tgtatcagag
     1021 gagcagcaat tgcctggtat cagggttcta tcctgactat tctcgccgca acagcaggtt
     1081 gagatgttcc ctgatgcagg aatgatcggc acggtacaac cgcaccccga acctgcagtc
     1141 tcgggaacgg cagaggacag agcgactgct ccacccgttt cattaccgca agggggagca
     1201 acggaatgat tccctgacgg caccgccaca accggttctg ctccagagga acagcagcat
     1261 ccagccgccg gattgggtgc agctgcctct cctggagtgg tgatagcggg gaggggcata
     1321 ccttccccag gagatgctcc gtgatcagag gagctgcgcc aagtcaggag acgcagggca
     1381 ttcaggatca cgaagaccgc actcgcctcg ttcaagagca ggcccgtgac cagtccaaga
     1441 tacccaagca gggctgcgat gaccatgaac gcaacgttga tcagggagac tgcgatgttc
     1501 tgccggattg tcgtgaccgt ccggtgcgaa agctcccgta tgaacgcaat cttcccgaga
     1561 tcatcggaca tcagcacgat atccccagcc tcgatcgcga tatccgtgcc ggcagccccc
     1621 atggcaattc caacgttggc gacggccatg gccggggcat cgttgatccc atcgccaacc
     1681 atcgccacgg tcccgtgttc ctccttgagc tggcggatca catcgacctt gtctgtcggg
     1741 aggagttgcg cccggtactc atcgactcct acgcgatgag cgattgccct tgcactccgg
     1801 tcgttatccc cggtcagcat caccgtcctg actcctgcac gggagagccg ctgcactgca
     1861 tcgaccgcac ccggcctcac ctcgtcggca attgcgatca ggccgatcag gtatccatca
     1921 cgtccaacca gcacaaccgt cctgccctcg tcttcgaagc gggcaatggt ttcgccagct
     1981 tcatcgatgc tgatgccctg ttcgctcagc gacctaggac ttcccagcac acaagaacgc
     2041 tcgccaacgt tcgctgcgac accacgtccg gcggtctcat gaaacccctc caccagctgg
     2101 tctgggaggg ggccgctctc ccgtgccttc cggacaatgg cagcagcgag cgggtgcccg
     2161 gactgtacct cgatacaccc ggcgagttcg agtacttcgt ccggggtaac atcgccaaat
     2221 gcaaggatgt ccgtgacctc gggtcgcccg atcgtcaggg tcccggtctt atcgaaggca
     2281 atgaccctta ccttgtcagt gacctccagg tacgtccctc ctttgaagac cgtgccatgc
     2341 ctggctgcgt tggccatcgc cccgaccacg gctacgggga ccgagagggc aaggccgcat
     2401 gagcaggaga caacgaagac gacgagccct cggtagatga acggatacca cggggccccg
     2461 aagaagagag gcggtacgat ggctaccatt acgccgagga cgaacattgc aggggtgtac
     2521 catttcccaa acgagtccgc gaaccgctgg taggatgtcc gttttgcctg tgcttcctca
     2581 accgacatga tgatccgcga gagcatcgtc tcggatactg gttttgtgac cctgacctcc
     2641 agcgatccgt tctggttgat ggtcccggca aatacttcgt ctcctgactg tctgttcacg
     2701 gcgattggtt cgcccgttac cgcagcctgg tcgacaaacg atgtccccga tatcacggtc
     2761 ccgtccaccg gaattcgctc acccggccgg accctgacga cttcaccaac cccgatcgta
     2821 tcaacggcac agattatttc ccggccgttc cgccggacaa gagcctcctt tggcacgagc
     2881 gccatcaggg agcgaatggc accccgggct ttatccacgg catatgactc gagcacgtct
     2941 ccaagtgagt agacgaagat caggactgcc gcttctcccc agaggccgag cagcatcgca
     3001 cccacagagc cgatgagcat cagaaggtga atggtcgggg tcaggttgcg cagtgcaatg
     3061 agggcttttc gtgccggata gaatatccca acgagcactg ctagaagata caatccgttg
     3121 acgaggatca gagatgcccc gaaatacccg gcaataaatg caacaagggt gataaggccg
     3181 catccataga gagcgagttg ctgtggttcc cgccaccatg tgctcctccg cccctcgcta
     3241 cggacgaggg atgcggtcat tcccgtctcc gaaaccgtgc ggatgatttc ctgaacactg
     3301 acaaccgcgg gatcgtaggt gattcttgcc tgcccagtga caggagtgat ctcctggcct
     3361 tttattccgg caagagagtt agcttccggg cgagcagatc agcattgcag gtgcagt
//
LOCUS       DAOQYR010000155         3412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_29034_length_3412_cov_9.563896, whole genome shotgun sequence.
ACCESSION   DAOQYR010000155 DAOQYR010000000
VERSION     DAOQYR010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29034_length_3412_cov_9.563896"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..728
                     /locus_tag="PKM87_11705"
     CDS             48..728
                     /locus_tag="PKM87_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HNQ30777.1"
                     /translation="MDEQESDLSALRIIAENRRGVLRDISTVVAGHNANILMIHQEIF
                     DSGPWDGMAELYIEFDACSDSAAMMSHLGEIPSVREVCSVEPFSHIYGTRVIIIGGGA
                     QVAQVALGAVNEADRHNIRGERISVDTIPLVGEKTVAQAVDAVARLPRAAILVLAGSL
                     MGGEISKAVDRVREAGIPVIALKMAGSVPQHVDMVVTDPIQAGVFAVMHVSEKAVFDI
                     NRVRGKEF"
     gene            730..1305
                     /locus_tag="PKM87_11710"
     CDS             730..1305
                     /locus_tag="PKM87_11710"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNQ30778.1"
                     /translation="MDPVERAVGILRQDGLVVYPTDTIYGLGADALSDEAILKVYEAK
                     RRPVAQPISVAVSDLEMAFGIARIDDLARAFMEAFFPGPVTVVVPSRSSLPEILCGGT
                     GQVGIRLPAHPVAIRLIKLLDSPITATSANLHGARDPVTPDECHVMHDLLIDGGKLPG
                     TPSTVVDLEQCLILRRGALADAVDAYLETLR"
     gene            1311..2243
                     /locus_tag="PKM87_11715"
     CDS             1311..2243
                     /locus_tag="PKM87_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNQ30779.1"
                     /translation="MRRKPIRLRDFIEDQDGWLYAVSTYDNQERAGCLLRYMPDSDGD
                     RVNAEGMRYRKVDFEESFELIKKEKPEYCDLLHRVPLEDIRRVLKPEEELPHIVARDH
                     RIYSLVSLFNLPPRTIGCTGSFLCGLEDTWSDIDLVVYGRQWFHAQELLKYATLKGKL
                     PAVDDETWKRIYAKRNPELGFEEFLLHEKRKWNRGQIGGTYFDLLYTRSYKALHPIPS
                     GKGDVMGMRVVEARVTDAHLSHDNPAVYEVDHDEVSRVLSFTHTYSGQALEGELIEAR
                     GVCEKHGDETWLIVGTTREARGEYIRSLTLLGRE"
     gene            complement(2275..2970)
                     /locus_tag="PKM87_11720"
     CDS             complement(2275..2970)
                     /locus_tag="PKM87_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HNQ30780.1"
                     /translation="MNKTLSGPDRVIPVSSVVTASLCPMRLYLEARSGEPRFESPKYT
                     ICKQVSSHLDGDPDAPQIWREVVAIVPDIDPGMYSWMEECLARCREGGPWAAFSDTDV
                     YFRSETHGLSGNLDKLHESDPFISIVRAVPAPRAGVYSADRLRVFGYMLLVSEATGRD
                     VRGGMVEYIPSGEARFCSPQPIDKRRFFKALSDARTILSGDSPPRPTGGRCRACESYG
                     ACSPSGRRLSDLL"
     gene            3169..>3412
                     /locus_tag="PKM87_11725"
     CDS             3169..>3412
                     /locus_tag="PKM87_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNQ30781.1"
                     /translation="MTSHEIAVNIPNTLDPVYSNMIQIAYKEDEFTFVFLHQIPGVNQ
                     ARAKAIVSITPQHAKTLLSVFSKTMADYESKFGKVEA"
BASE COUNT          720 a          982 c          991 g          719 t
ORIGIN      
        1 cccaagcctc taacccgggt tcgatccccg gctatcgcat acccgccatg gatgaacagg
       61 agtccgatct ctctgccctg cgcatcatcg cggagaacag aagaggggtt ctgcgcgata
      121 tctctactgt agtcgccggt cacaatgcaa atatcctgat gatccaccag gagatcttcg
      181 attccggtcc atgggatgga atggccgagc tctacataga gttcgatgcc tgctccgatt
      241 ccgcggcaat gatgtcacat ttgggggaga tcccatcggt aagagaggtc tgctccgtcg
      301 aaccgttcag ccacatctat gggacgagag tcatcattat cggtggaggc gcccaggtgg
      361 cacaggttgc cctgggagcg gtgaacgaag cggaccgcca caacattcgg ggagagcgaa
      421 tctctgtgga cactattcca cttgtgggtg aaaagaccgt tgcccaggcg gttgatgcag
      481 tcgcacggct cccgagggct gcaatcctcg tgctcgccgg gtccctgatg ggaggcgaga
      541 tctccaaagc tgtggatcgt gtcagagagg caggtatccc ggtgattgca ctgaagatgg
      601 cgggaagcgt tccccagcac gtggacatgg tggttaccga tccaatccag gccggggtat
      661 ttgcagtgat gcatgtcagc gaaaaggcgg tgtttgacat caaccgggtc aggggaaagg
      721 aattttgaga tggacccggt tgagagagcg gttggtatcc ttcggcaaga cgggctcgtg
      781 gtgtacccga ccgataccat ttacgggctc ggggcggatg ccctctctga tgaggctata
      841 ctcaaggttt acgaggcgaa aaggcgcccc gttgcccagc ccatctctgt cgcggtctct
      901 gacctggaga tggcattcgg cattgctagg atcgacgacc tggcacgtgc gttcatggag
      961 gcgttcttcc cgggaccggt gaccgtggtg gtcccgtcca ggtcctcgct cccggagatt
     1021 ctttgcggcg gtacagggca ggtcgggatc cggctgcccg cacatcctgt ggccatccgt
     1081 ctcatcaagc tcctcgactc tcccatcacg gccacgagtg caaacctgca tggtgcaagg
     1141 gaccctgtca caccagacga atgccacgtg atgcacgatc tcctcattga cggcgggaag
     1201 ctgcccggga cgcccagcac ggttgtcgac ctggaacaat gtctcattct ccgccgtggt
     1261 gctctcgctg acgccgtaga tgcatatctc gagacattgc ggtgagagcg atgaggagaa
     1321 aacccatccg tcttcgtgat tttatcgagg atcaggatgg gtggctgtat gccgtttcca
     1381 cctacgataa ccaggaacgg gctggctgtc tcctccggta catgcctgac tctgacggag
     1441 accgggtgaa tgccgaaggc atgcgttacc ggaaggtaga cttcgaggag tcgttcgaac
     1501 ttatcaagaa ggagaagcct gagtattgcg accttctcca ccgtgttccc ctggaagata
     1561 tccggcgggt tttaaaacca gaggaggagc tgccacacat cgtcgcacgc gatcaccgga
     1621 tctattctct tgtatcgttg ttcaacctcc cgcctcgaac catcgggtgt accggctcgt
     1681 ttctctgcgg cctggaggat acctggtccg atatcgatct tgtggtgtac ggccggcagt
     1741 ggttccatgc ccaggaactg ctcaagtatg caacgctgaa aggaaagctt ccggctgtcg
     1801 acgacgaaac ctggaagagg atttacgcga agcgaaaccc tgaactcggc ttcgaggagt
     1861 tcctcctgca cgagaagagg aagtggaacc gcggccagat cggagggact tacttcgacc
     1921 ttctctatac ccgctcctat aaagcgcttc atccgattcc gtccgggaaa ggggatgtca
     1981 tggggatgcg tgttgtcgag gcgcgggtaa cagacgccca tctctcacat gacaatcctg
     2041 cggtctacga ggtggaccac gacgaggtct cacgtgtcct ttcctttacc catacctaca
     2101 gcgggcaggc actggagggc gaacttatcg aggcccgggg tgtctgcgag aagcatggcg
     2161 atgaaacctg gctcatcgtg gggacgaccc gggaagcccg cggagagtac atccggtccc
     2221 tgaccctcct tggccgggaa tgaggcgggt ttcctgcgga tgacgcatcc tttttcacag
     2281 gagatccgag agccggcgcc ctgatgggct gcatgctccg taggattcgc atgcccggca
     2341 tctcccccct gtcggcctgg gaggactatc tccagagagg attgtccttg catcgctcag
     2401 cgccttgaag aatcgccgct tatcgattgg ctggggactg cagaatctcg cctcaccgga
     2461 ggggatgtac tcgaccattc cgccgcggac atccctcccc gtcgcttcgc tgacgagcag
     2521 catgtacccg aataccctga gccgatccgc cgagtagact cctgcccggg gtgcggggac
     2581 cgccctgacg atcgatatga agggatcgga ctcgtggagc ttgtccaggt tccctgaaag
     2641 accgtgcgtc tccgaacgga aatatacgtc agtgtcggag aacgccgccc aggggcctcc
     2701 ctccctgcac cgtgcaaggc attcttccat ccaggaatac atcccagggt cgatatctgg
     2761 gacgattgca acgacttcac gccaaatctg cggggcgtcg ggatcaccgt ccaggtgcga
     2821 ggagacctgc ttgcagatgg tgtatttcgg gctctcgaag cgtggctcac ccgatcgtgc
     2881 ctcgaggtac agccgcatgg ggcagagcga tgcggtcacg acagatgaca ccgggattac
     2941 ccggtcaggc cccgataatg ttttattcat gtgaaaaagc tctcaaatcg tctttaaatc
     3001 accgcatatc cattcatccg gccccatcca cacaatgcag ggaaggggcc agccgcgtgc
     3061 cagaagcctc tcgtgaacac tggcttctcc caggcatgtc ccgggtctct ttttggggat
     3121 tcaatcactc gcaaaggatt aacctgtttc cattctcaca gtacttacat gacatcacat
     3181 gagattgcgg tcaatatccc caacaccctg gacccggtat acagcaatat gatccagatt
     3241 gcctataaag aagacgaatt cacatttgtc ttcctgcacc agatcccggg cgtgaaccag
     3301 gcgcgggcaa aagccatcgt cagcatcacc ccccagcatg caaagaccct tctctctgtc
     3361 ttctcaaaga cgatggccga ttacgagtcg aagttcggca aagtggaggc cg
//
LOCUS       DAOQYR010000156         3302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_30465_length_3302_cov_9.646751, whole genome shotgun sequence.
ACCESSION   DAOQYR010000156 DAOQYR010000000
VERSION     DAOQYR010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30465_length_3302_cov_9.646751"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..967)
                     /locus_tag="PKM87_11730"
     CDS             complement(122..967)
                     /locus_tag="PKM87_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNQ30782.1"
                     /translation="MIVKDYMTSDVVTVEIPGNRDDVLKILKRTGISGVPVLKDADLV
                     GIITRKDLLRKAEETQLGLLMTPNPVTIGPDSTVREAASMMVRHSVRRLPVIDDGSLV
                     GLLSVADLIAALAQMKIKDEIKDLYTSQTFAIWEETPLPLVGRIMEISGFEAIPILDA
                     ESTLQGIISERDLIRNSSIEDMVEVSDFSNGTDDDEWTWESIRDMHTISYGISKIQLP
                     DRPVKTAMVRNVISVPMNAEVSECALKMKRARVDQLPVVNGDKKLVAMLFDRDLIRAL
                     CQKDL"
     gene            complement(964..1401)
                     /locus_tag="PKM87_11735"
     CDS             complement(964..1401)
                     /locus_tag="PKM87_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNQ30783.1"
                     /translation="MFSEILVAVDGSKIGKRALEVAIEEARLWKARVHVVYVVETGLF
                     SSLPMDSTWEIMYSMLEKEGQETISDAKEQAAAKGVRLETYIRQGHAGNEIIKASREL
                     GADLIVVGSHGKSEVDRLLLGSVSSFVVGNSSVAVLVVRPSQK"
     gene            complement(1439..2488)
                     /locus_tag="PKM87_11740"
     CDS             complement(1439..2488)
                     /locus_tag="PKM87_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNQ30784.1"
                     /translation="MAAGELLVEGRALLGEDLEEAPVAVVVKGGIIHRIEHLSSTPAN
                     APWICPAFFNAHTHLGDSLAMDLPFEGTLDEVVRPPNGLKHRILRSAARKDLLAGMHL
                     TLLTMAESGTAGCADFREGGEDGVSALREAARGVPCRTIIFGREGGEAIADGLGISSA
                     RDASAGLEEAVSAAKRSGKMVAFHAGERDAGDVDEALEYHPDLLIHCTHATRSQLRRI
                     ADLGIPVAVCPRANWRFGVTSSRDHPPIETMQALGCQILLGTDNAMCVQPDMWREAHF
                     LSFVYRVTPRDALKSATLGSSLFCEPFFLKEGKKANFLVIDPGKSNLAISRDLVGTLV
                     NRAGPENIVRKVINL"
     gene            2535..2693
                     /locus_tag="PKM87_11745"
     CDS             2535..2693
                     /locus_tag="PKM87_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit Sec61beta"
                     /protein_id="HNQ30785.1"
                     /translation="MAKKQGGRLISSAGLVNYYESEDKRAFHISPYSVIIGCAAIGVA
                     ILVLNMIY"
     gene            2842..3108
                     /locus_tag="PKM87_11750"
     CDS             2842..3108
                     /locus_tag="PKM87_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1922 domain-containing protein"
                     /protein_id="HNQ30786.1"
                     /translation="MFLIIRCPGCRTFGYVDRFQQWKLCPVCGEAIDVRQATAYMEVD
                     EYQLAERIVAQLEEYLHRNKKKDFTPDEIRELREQYAQWIRTKA"
     gene            complement(3118..>3302)
                     /locus_tag="PKM87_11755"
     CDS             complement(3118..>3302)
                     /locus_tag="PKM87_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30787.1"
                     /translation="PFSPTLFHTLPMVRHECGYEQEIFCRRCGTPVVYNERTGLQCPK
                     CGHEITLLCHGCGKKW"
BASE COUNT          668 a          966 c          917 g          751 t
ORIGIN      
        1 ttcattcctt cctggtatct ctcattcatt cacacacccc aaaatattcg taaacgccag
       61 taaaatgctc gaaaagcgct tctaaacaat acaatgtcct tataaaattt aaaagttgtt
      121 atcagagatc cttttggcag agggcgcgga taagatccct gtcaaagagc atggcgacca
      181 gtttcttgtc cccgttcacg acggggagct ggtccacgcg cgcccgtttc atcttcaggg
      241 cgcactcgct cacctcggca ttcatgggca ccgagatcac gttcctgacc atggccgtct
      301 tgaccgggcg atcggggagc tggatcttgg agatgccgta actgatggtg tgcatgtccc
      361 ggatactctc ccaggtccac tcgtcatcgt cggtcccgtt cgagaaatcg ctgacttcca
      421 ccatgtcctc tatcgatgag ttccgaatca ggtcacgctc ggagatgatt ccctggagcg
      481 tgctctcggc atccaggata gggatcgcct cgaacccgga gatctccatg atccttccta
      541 ccaggggaag gggggtctct tcccagatgg caaaggtctg gctggtgtaa aggtccttta
      601 tctcgtcctt tatcttcatc tgggcaaggg cggcgatgag gtccgcaacg gagagcaggc
      661 caacgaggga cccgtcgtct atgaccggca gtcgccggac cgaatgcctg accatcatgg
      721 aggctgcttc ccggactgtt gaatcggggc cgatggtgac ggggttgggg gtcatcagga
      781 gaccgagctg ggtctcttca gcctttcgga gaaggtcttt ccttgtgatg attcccacga
      841 gatctgcatc cttcagcacc ggtaccccgg atatgccggt ccgcttgagg atcttcagga
      901 catcgtcgcg gttcccggga atctccacgg tcaccacgtc tgatgtcata tagtctttga
      961 cgatcatttc tgggatggtc tcaccaccag gaccgcaacg ctgctgttcc ccaccacgaa
     1021 cgagctcacg cttcccagga ggagccggtc aacctcgctc ttcccgtgcg agcccacgac
     1081 gatcaggtct gcgccaagtt cccgtgatgc cttgatgatc tcatttcctg cgtggccctg
     1141 cctgatgtag gtctcgagcc tgacaccctt tgcggcagcc tgctcttttg cgtcggagat
     1201 tgtctcctgc ccctctttct cgagcatgct gtacatgatc tcccacgtgc tgtccatcgg
     1261 gagcgaggag aacaaccccg tctccacgac gtacacgaca tggaccctgg ccttccatag
     1321 ccgggcttcc tcgatcgcga cctcaagcgc cctctttccg attttagacc cgtctactgc
     1381 taccaatatc tccgagaaca tggttaccct ccaaaacagt ctccttaatt aatgtcatct
     1441 ataaatttat aacctttctt acgatatttt ccgggcccgc ccggtttacc agggttccta
     1501 ccagatcgcg gctgatcgca aggttcgact tgcctggatc aatgacgagg aaattcgcct
     1561 tcttaccttc tttcaggaaa aatggttcgc aaaagagcga cgaaccgagc gttgcggatt
     1621 tcagcgcgtc gcggggggtg acacgataga caaaagagag gaagtgggcc tccctccaca
     1681 tgtcgggctg cacgcacatg gcattgtcgg ttcccagaag tatctggcat cccagtgcct
     1741 gcatcgtctc aatcggcggg tgatcgcgcg atgacgtgac accgaacctc cagttcgccc
     1801 ttggacagac cgcgacaggg ataccaaggt cggcaatcct gcgaagctgg gaacgggttg
     1861 cgtgcgtgca atggatgagg agatcgggat ggtactcgag ggcctcgtcc acatccccgg
     1921 catccctctc gccggcatgg aatgcgacca tcttcccgga tcgcttcgcg gcggagactg
     1981 cctcttccag gccggcgctt gcgtccctgg cgctgctgat cccgagtccg tcggcgattg
     2041 cctcgcctcc ttccctcccg aaaataatcg tcctgcatgg gacacccctc gctgcctccc
     2101 ggagtgccga taccccgtct tccccgccct ccctgaaatc tgcacagccg gcagttccgg
     2161 attcggccat ggtgaggagg gtgaggtgca tcccggcaag gaggtctttc ctggcggcag
     2221 aacggaggat ccggtgcttg aggccgttgg gaggccggac tacctcgtcg agtgtgccct
     2281 cgaagggaag gtccatggcc aggctgtcgc cgaggtgggt atgggcattg aagaaagcgg
     2341 ggcatatcca cggagcgttc gcaggagtgg acgacaggtg ctcgatccgg tggatgatcc
     2401 cgcccttcac caccactgcc acgggcgcct cctcgagatc ctcgccaaga agcgcccgac
     2461 cctccaccag gagttctcct gcggccatgc gtgctgactt gcttttatat accgagaatg
     2521 agaaatatgt ttgtatggcg aagaagcagg gtggacggct tatctcctca gcgggactgg
     2581 tcaattacta cgagagcgag gacaagaggg cctttcatat cagcccctac tcggtaatca
     2641 tcgggtgcgc cgccatcggg gtggcgatac tcgtgctgaa catgatctac tgaataattc
     2701 ttatcttttc tgtgagtttt ccatggattg tgtgatgtcc atgggtgccc ggtcatcgcg
     2761 agctgtgcag gcgcaatcac ccgggtgccg gtcattcggc gatatatata tactatagga
     2821 agggacaatc acccattgtc gatgttcctg atcatccgtt gcccgggatg ccggaccttt
     2881 ggatacgttg accggttcca gcaatggaag ttatgccccg tgtgcgggga ggcgatcgat
     2941 gtccggcagg ccaccgcgta catggaggtc gacgaatatc agcttgccga gcgtattgtt
     3001 gcgcaactcg aggaatacct tcaccggaac aagaaaaagg acttcacacc cgacgagatc
     3061 agggagctcc gggaacagta tgcccagtgg attcgcacaa aggcatgaga gctccgttta
     3121 ccactttttc ccgcacccgt ggcagagaag cgtgatctcg tggccacatt ttgggcattg
     3181 gagcccggta cgctcgttat agaccaccgg tgtcccgcac ctcctgcaga agatctcctg
     3241 ctcgtacccg cactcgtgcc tgaccatagg aagggtatgg aagagggttg gagaaaaagg
     3301 tt
//
LOCUS       DAOQYR010000157         3228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_31490_length_3228_cov_11.282383, whole genome shotgun
            sequence.
ACCESSION   DAOQYR010000157 DAOQYR010000000
VERSION     DAOQYR010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31490_length_3228_cov_11.282383"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PKM87_11760"
     CDS             <1..486
                     /locus_tag="PKM87_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30788.1"
                     /translation="FIVHPERVAKCVAHYGGNHVGIIAAWLHDVIEDHAEGDAIVREA
                     LEETCLPENNLHEIYAIVLALTKNPAIPGKRERFADTLDRILHAPGQAILVKLCDRMD
                     NLIDARDQGQQFLSEYLDLTDHLIATLSDGAIRHGYSEALGHLKVVREDLGFFYEAKF
                     F"
     gene            complement(559..1176)
                     /locus_tag="PKM87_11765"
     CDS             complement(559..1176)
                     /locus_tag="PKM87_11765"
                     /inference="COORDINATES: protein motif:HMM:NF025432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HNQ30789.1"
                     /translation="MNLIVIDDVKKTDEYWIMPPLKKTLPPEQQNELLRVVKARFEKH
                     MDRHTGLLWKNVQARLEAHPEKLWSLHEMERTGGEPEVVGHDKKTGEYLFYDSSPESP
                     KGRRNVCYDREGQEARKTNTPDNNAIDMAAAMGIELLTEEQYRALQDLGNFDTKTSSW
                     IKTPPAIRQLGGALFAHRRYGRVFLFHNSAPSYYAARGFRGSLRV"
     gene            complement(1183..1584)
                     /locus_tag="PKM87_11770"
     CDS             complement(1183..1584)
                     /locus_tag="PKM87_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNQ30790.1"
                     /translation="MKWTQREVHTSARIQLLDITGVVASELEKSGIKTGVCHVYVPHT
                     TAGLTINENADPDVARDILTTLNRLVPATGDYRHSEGNSDAHVKASLMGFSAIVPVVD
                     GHLAFGTWQAVYFCEFDGPRHRHVLIGISGE"
     gene            1941..2207
                     /locus_tag="PKM87_11775"
     CDS             1941..2207
                     /locus_tag="PKM87_11775"
                     /inference="COORDINATES: protein motif:HMM:NF020317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HNQ30791.1"
                     /translation="MHFVALLKFKKKPDKAFMIETQKLIDADTQKGFKFHAIYWILGK
                     YDAVAVYEAPDEKEAMKAAMKRVELMDVETYVAIPREEVKRLVE"
     gene            2326..3036
                     /locus_tag="PKM87_11780"
     CDS             2326..3036
                     /locus_tag="PKM87_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNQ30792.1"
                     /translation="MKPMSLAGSLFILSMLLLSGCTGNKVIPPPVQETVSAEGSTISL
                     PSPRIQSQTSVEAALLTRRSVRTYSDTPLTLPEAGQLLWAAQGITDPNGFRTAPSAGA
                     LYPLELYLVVQSVDRLSPGIYHYLPTDHQLRLLSEGEVSDQLSSAALRQSAVKDAPVV
                     IVFSAVPERTTARYGERGMQYVFMEAGHAAQNVCLQAVALDLGTVTIGAFDEDEVRTI
                     LNLPEREIPLYLLPVGRP"
BASE COUNT          672 a          883 c          872 g          801 t
ORIGIN      
        1 ttcattgtcc atcctgagcg ggtggcgaag tgcgtcgccc attacggggg gaaccatgtc
       61 ggcatcattg cggcctggct ccatgacgtg atcgaggacc atgctgaggg ggatgcgatt
      121 gtccgtgaag cactggaaga gacgtgtctt cccgagaata acctgcatga aatctacgcc
      181 atcgtcctgg cccttaccaa gaatccggca attcccggga aacgggagcg gtttgccgac
      241 acgctggacc ggattctcca tgctccaggc caggcgatcc ttgtcaagct ctgtgaccgg
      301 atggataacc tgatcgatgc ccgcgatcag gggcagcagt tcctctccga atacctggac
      361 ctgacggatc acctgattgc tactttatct gatggtgcca ttcgtcacgg gtattctgaa
      421 gccctcgggc acctgaaggt cgtccgggag gatctgggtt ttttttacga ggcaaaattc
      481 ttttaatcca tggcatgggg agagggaagg cgagggggtc ccctcccaaa ccgggcgaga
      541 gtgagaaagc ccaccagttc agactcggag cgagccacga aatccccttg cggcatagta
      601 cgatggcgca ctgttgtgga agagaaagac cctgccgtac cggcgatggg caaagagggc
      661 cccgccgagt tgtctgatcg cgggaggggt ctttatccag cttgacgttt tcgtgtcgaa
      721 attacccagg tcctgcaacg cccggtattg ctcttccgtt aacaattcaa tgcccatggc
      781 ggctgccatg tcaatcgcat tattgtctgg tgtattggtc ttcctggcct cctggccttc
      841 acgatcgtaa cacacgttcc tgcggccttt gggactctcc ggcgaagaat cataaaagag
      901 gtattcgcct gtttttttat catgaccgac cacctccggc tcccctcccg ttcgttccat
      961 ttcatggagc gaccacagtt tttcgggatg tgcttccagc cttgcctgga cgttcttcca
     1021 gagaagacct gtatggcggt ccatgtgttt ctcaaaacgg gcttttacta cccttagtag
     1081 ttcgttctgt tgttccggtg gcaaggtttt cttgagcgga ggcatgatcc agtattcatc
     1141 cgtttttttt acatcatcga tgacgatgag gttcatcccc ttctactcgc cactgatccc
     1201 gatcagcacg tggcgatgac ggggaccgtc aaactcgcag aagtacacgg cctgccaggt
     1261 tccgaaggcc aggtggccat caacgaccgg aacgatcgcg gaaaagccca tgagcgatgc
     1321 cttgacatgg gcatcggaat tcccctcgct gtggcgatag tcgcccgttg ccggcaccag
     1381 cctgttgagg gtggtgagga tatcgcgggc gacgtcaggg tcggcgttct cgtttatcgt
     1441 aaggccggca gtcgtatggg gtacatacac gtggcagacg ccggttttta tgccgctctt
     1501 ttccagctca gaggcgacca ccccggtaat gtccagcagc tggatcctgg cgctcgtgtg
     1561 tacttctcgt tgtgtccatt tcatgatctc accttatgtg ccaccagtct gcccgcctta
     1621 tgggggggag ctggtctgat tccaatcggg ttgtgggtat caccatgtcc acgcatcgtc
     1681 tctccactga tcatcagtga tcatcctcat gatattcttt acctccccat tctcctgaat
     1741 ggccggggtg gcacaaagaa gggacgtgtt ccgtgtcctc tccaacatga gttccgtgtc
     1801 ctctccaaca tgagttccgt gtcctttcca ataggaatag tgcagtcatg tcccttcttc
     1861 cccggatgtt ttcaggtacc ttttaatagt caatctcata tgttaccatg cccttgaaaa
     1921 gggagaagga gtgatgaccg atgcattttg ttgcactgct gaaattcaaa aagaagccgg
     1981 acaaggcgtt tatgatcgag acgcagaaat taatcgatgc agatacccag aaagggttca
     2041 aattccatgc tatttactgg attctcggga aatacgatgc cgtggcggtg tatgaagccc
     2101 ctgatgaaaa agaggcgatg aaagcagcca tgaaacgggt ggaactcatg gatgtggaga
     2161 cctatgtggc tatcccacgg gaagaagtga agagactggt ggaatagaag aggattcagg
     2221 catttcccga tttttttcca gtggctcccc agtcatccac cacatcctct ccagtatgac
     2281 aaaagataat tatgcccctt ctgatatgca ccatgggaaa atgcgatgaa acccatgtct
     2341 cttgcaggga gcctgttcat tctctcaatg ctcctcttgt ccgggtgtac gggaaataag
     2401 gtgattcctc ctcctgtaca ggaaacagta tctgcggaag gatcgaccat ttctctcccc
     2461 tctccccgga tacagagcca gacctcggta gaagccgccc tgctcacccg ccgatcggtt
     2521 cggacgtata gcgatactcc gctcacgctc ccggaagctg gccaactgct gtgggccgcc
     2581 cagggaataa cggacccgaa tgggtttcgg accgccccct cagctggtgc cctctacccc
     2641 cttgagctgt accttgttgt acagagtgtt gaccgtcttt ccccgggaat ctatcactat
     2701 cttcccaccg atcatcagct ccgtctctta tccgaaggtg aggtatcgga tcaactgagt
     2761 agtgctgctc tccggcagtc tgcggtgaag gacgctccag tggtgattgt tttctccgct
     2821 gttcctgaac ggacaacggc caggtacggt gaacggggga tgcagtatgt cttcatggag
     2881 gcggggcatg ctgcccagaa tgtctgcctc caggcagttg ccctcgatct cggtacggtg
     2941 accatcgggg catttgatga ggatgaggtc cgaacaattc tgaacctgcc ggaacgtgaa
     3001 attccgctct acctccttcc ggtcggacgg ccttgataga aatgtatccc ttgcaggagc
     3061 acctttcagg aacctcgagg aacctcatcg cagatgggat cggcgaggat gcacacggac
     3121 gatctctttt tcggcacgat ggagggggcg agggcttctg tacgtatcat ctcagggatg
     3181 ctgctcttcc cttctgacct tcgctgcctc ccgttcgcga agctcatt
//
LOCUS       DAOQYR010000158         3182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_32165_length_3182_cov_8.740646, whole genome shotgun sequence.
ACCESSION   DAOQYR010000158 DAOQYR010000000
VERSION     DAOQYR010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32165_length_3182_cov_8.740646"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..463)
                     /locus_tag="PKM87_11785"
     CDS             complement(239..463)
                     /locus_tag="PKM87_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30793.1"
                     /translation="MRGEVEEHHFGPDEEITLLLYPEAAYLKDDIDGVRILFTGFHEK
                     PHAVEEARRMVEYHQLTEERLKSFTKGDKN"
     gene            726..932
                     /locus_tag="PKM87_11790"
     CDS             726..932
                     /locus_tag="PKM87_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFYB/HAP3 family transcription factor subunit"
                     /protein_id="HNQ30794.1"
                     /translation="MADLPIAAVIRIAKKSGAERVGSDAAVALVAQTEDYIACLVKEA
                     NTLAIHAGRKTIKKEDIDLAEKSM"
     gene            complement(1155..2465)
                     /locus_tag="PKM87_11795"
     CDS             complement(1155..2465)
                     /locus_tag="PKM87_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNQ30795.1"
                     /translation="MFYLIEILIIITLIALNGIFAMSEFAIVSARKMRLRQRAEKGDT
                     RAATALDLANEPTTFLSTIQIGITLIGIFAGAYGGVTIAKGLATSFTSFPSLVPYAEM
                     LSVTLVVLAITYLTLVFGELVPKRIALNNAEDLAVSIAKPMRFLSFIALPFVFVLSRS
                     TDLVLRIIGVQAPTEPPVTEEDIKLLLEEGTETGIFEKAELSMVEGIFDLADRRVESL
                     MTPRPDIIALDLDDPDDENLRKMVLSGRSNFPAFHDDLDTIVGMVSVKNVLAGMVEKG
                     SPDIQAAVTDPLFVPETISVLKLLESFKETGLHSALVTDEYGSVQGLVTLHDILEAIV
                     GDVRTLGEPEEIPVITREDGSWLIDGSTPVDDLKDILSVEVFPGEEEGEYRTIAGLIM
                     YILDRIPAPGDHFEIGGLRYEVVDMDGKRVDKVMVTRVSQTPAN"
     gene            2684..>3182
                     /locus_tag="PKM87_11800"
     CDS             2684..>3182
                     /locus_tag="PKM87_11800"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNQ30796.1"
                     /translation="MREQYGRNELTPPRRTPLWIQFLSKFNDPIIRILLVAVFISAIV
                     AVMEGGSLIDTIGIVMAVLLATSISFVTEYRSNREFEALNAMREDTGIKVIRDDHAAT
                     VPMRDLVVGDLVLLEAGDVVPADGYLLQASNFEVDESAFTGESEPLGKTVEEVVLKGT
                     LVTAGR"
BASE COUNT          799 a          817 c          785 g          781 t
ORIGIN      
        1 ctatcacctg tatcgcagcc ataagaaaac ggtgtccgtt cgcctcacga ccttgaaaat
       61 cgggcagcct gttcctgtgc aggagggtgc aggctgacct gagaatagtg cggatctgtt
      121 cttttctctc taaaagaggg ttttctattg tgtaggaaaa taaacggaga ttccgagcgg
      181 ttaccgaatc agatagcgga aatgtgtatc aacgaacatc taagagtatt ccagatactc
      241 agtttttatc tcctttcgta aatgatttca ggcgttcctc cgtgagctga tggtattcca
      301 ccattctcct cgcctcttcc actgcatggg gtttttcatg gaatccggtg aacaggatgc
      361 ggactccatc gatatcatcc ttcagatagg cagcttcagg atagaggagg agcgtaatct
      421 cctcgtccgg tccgaagtga tgctcttcta cctctcctct catatatcgt tcccgttgct
      481 gcctggtcgg tgcagcatat ttattttcaa atctgaaaat tcccatgaag gttcctcaca
      541 caatattagg gtaaaagtga ggttctcatt atgtggtgtt taaatctata gcaggcaggt
      601 tgaaagtgaa cacatgagat aagtggtcta aaattcatcc caatcaaatt ttgctgatat
      661 attgaagcaa ttttaaggga atttataaat actctctcac gttttatagt tttgaggtaa
      721 tcaaggtggc agatttacct attgcagccg ttataaggat tgcaaagaag agtggagccg
      781 agcgggtcgg aagtgacgcc gcagttgcgc ttgtcgcaca gaccgaagac tacattgcat
      841 gtcttgtgaa agaggcaaac acgcttgcaa tacacgcagg aagaaagacc atcaagaaag
      901 aggacatcga ccttgcggaa aaatcgatgt aatcctcact ctttcttttc gcgtgtgttt
      961 cttgtttttc cttctgattc tttgtaaaaa gagtcgcatt cttgttgtgt tacaccctga
     1021 cgcttgaagg aggggctgtc gccccccgcc gcgtgggcgc cgccctgtga atcaagatca
     1081 tcctgggaac ggagcatcgt gctccgttca cttctccttt actcaactcc cccctaacca
     1141 agccgaagat accctcaatt ggcgggagtt tgagagaccc ttgtcaccat caccttatcc
     1201 acgcgtttcc catccatatc caccacttca tagcgcagac ccccgatttc aaaatgatca
     1261 cctggtgcgg gaattctatc caggatgtac atgatcagcc cggcaattgt tcggtactcc
     1321 ccctcttctt cacccgggaa aacctcaacc gataaaatat ccttgagatc gtcgacaggg
     1381 gtgcttccat ctatcagcca ggaaccatcc tcccttgtga taacagggat ctcttccggt
     1441 tctcccaacg ttctgacatc cccgacaatc gcctcaagaa tatcgtgcag ggttacgagt
     1501 ccctggacac tgccgtattc gtcggtgacc aacgcactgt gcaacccggt ctccttgaag
     1561 gattcgagaa gtttcaatac cgagatggtt tccggaacaa ataacgggtc cgtcactgcc
     1621 gcctggatgt caggcgagcc cttttccacc atcccggcca gaacattctt gacggacacc
     1681 ataccgacaa ttgtatcaag gtcgtcatgg aatgctggaa aattagaacg gccgctcaaa
     1741 accatctttc gcaggttctc gtcgtcgggg tcgtcgagat cgagggcaat aatatccggg
     1801 cgtggcgtca tcagcgattc gacccggcgg tctgcaagat cgaatatccc ttccaccatg
     1861 ctcaattcag ccttttcaaa gatcccggtt tctgtcccct cttccagcaa cagcttgata
     1921 tcttcttcgg taacgggtgg ttcggtgggt gcctggacac ctataatccg taatactaaa
     1981 tcggttgaac ggctcaatac gaatacaaaa ggtagagcga tgaaagagag gaaccgcatc
     2041 ggtttcgcaa tggacacagc aagatcctcg gcgttattca gtgcgattcg cttcgggaca
     2101 agctccccaa agaccagtgt caggtacgtg atggcaagaa ccaccagggt cacactcagc
     2161 atttctgcat aggggacaag tgagggaaac gaggtaaagg aggtggccag tcctttcgca
     2221 atggtcacac cgccatacgc acctgcaaaa atcccaataa gtgtgatccc gatctggatg
     2281 gtcgacagaa acgtggtggg ttcattcgcg aggtcgaggg ccgttgccgc ccgggtatcc
     2341 cccttttcgg cccgttgccg gagccgcatc ttccttgcgg aaacaatagc aaactcagac
     2401 atggcaaaga tgccattgag agcaatgaga gtgatgatga tgagtatttc tataaggtaa
     2461 aacattttgc gatgagtgtc ggcgaatgta aatttatacc ttgctgataa agacgtttcc
     2521 aaaaattcta agtatttcga gagtttggca gggaaatttg ttccaggtat tttcacgcac
     2581 aaagatgaat acccaaaagg acaacactgg tagaatgatc ccattccggg acctcccaga
     2641 atctgcccga acacacggcc tttcttctca ctccgttcgt gaaatgcggg aacaatacgg
     2701 caggaacgag ctgactcccc caagacgaac accgctgtgg atacagttcc tctcaaagtt
     2761 caacgacccg atcatacgta ttttactcgt cgcagtcttc atttcggcga tcgtggcggt
     2821 catggagggg ggcagcctca ttgacaccat cgggatagtg atggcggtcc tgctcgcgac
     2881 cagcatctcg tttgtgaccg aataccggag caaccgcgaa ttcgaggccc tgaacgcgat
     2941 gcgggaagat acgggtatca aagtgatccg ggacgaccat gcagccacgg tccccatgag
     3001 ggacctggtc gtgggagacc tggtcctcct ggaagcaggc gacgtggtcc cggccgacgg
     3061 gtatcttctc caggcatcga atttcgaggt ggacgaatcc gcgttcaccg gggagagcga
     3121 accactcgga aaaacagtcg aggaagttgt cctgaaaggg accctggtca ctgccggccg
     3181 gg
//
LOCUS       DAOQYR010000159         3094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_33564_length_3094_cov_7.231655, whole genome shotgun sequence.
ACCESSION   DAOQYR010000159 DAOQYR010000000
VERSION     DAOQYR010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33564_length_3094_cov_7.231655"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PKM87_11805"
     CDS             complement(<1..225)
                     /locus_tag="PKM87_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ30797.1"
                     /translation="MNADAFIIDGYVDEPACLGVPPYISPYIRTIAGVFRAHGIEPRY
                     ITIDQIREDPRILTPVPGGIAIMIAGVTVPG"
     gene            complement(215..835)
                     /locus_tag="PKM87_11810"
     CDS             complement(215..835)
                     /locus_tag="PKM87_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNQ30798.1"
                     /translation="MEFEEWEPYYQEILDYFGIVREDDEKAARILASLIGKDDCSLLS
                     SLCSGRIVTVCGNAPTLPYETEHIEGSIFAADAAAGVLYESGIRPNAVFTDLDGASES
                     FLAMNREGTIMVIHAHGDNIPQLQYWVPLFPGPVVGTTQSQPFPPLHNFGGFSDGDRA
                     AFAADALGAERIVFVGFDLDDMNVGHMKRGKLLWARRLLRLIGYEC"
     gene            complement(825..2291)
                     /locus_tag="PKM87_11815"
     CDS             complement(825..2291)
                     /locus_tag="PKM87_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNQ30799.1"
                     /translation="MADSAQNGSRERVLYAVLGCGSTGYHVLQELQSSTGNVLVIDKN
                     EDRVRELRDQRIDAVRRDIFAVDFLSGIPAIEIAFVLTGDNEANLAAVKALRETYPHS
                     RIMARASDPLVARNLEEAGADFVLNQNQLMAKAAVYQINKVYANRVSQRLYSLLSSWE
                     GTLGIITHKNPDPDAISSALALAVIAKNANPNRLVCRIFYDGIIGHQANRTLVNLLEI
                     KMEKMDPAVLQECTYLALVDSPAPGVNNTLPPKTRVHIVIDHHEDGKLAESPAVFVDS
                     RPGIGATASILSQYLQELDIGVDKRVATGLLYGIRSDTRDFSRNVTPQDLINAAFLLP
                     LTDNSLLDQIMSPSLSEETLDILGIAIANRKIQSGYLFSNVGFIRNRDALPQAADLLI
                     TLEGVNTALIYGISDEAIIMSARNRDVRLHIGNVLKEAFGTMGEAGGHPTMAAAVIPL
                     SFFSLAKSKADLLSLVTEPLLGRFANIVGLESEVRNGI"
     gene            2456..2529
                     /locus_tag="PKM87_11820"
     tRNA            2456..2529
                     /locus_tag="PKM87_11820"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2490..2492,aa:Lys,seq:ctt)
BASE COUNT          788 a          767 c          803 g          736 t
ORIGIN      
        1 gccgggtacc gtcacaccgg ctatcatgat tgcgataccg ccggggacgg gagtaagaat
       61 gcgtgggtcc tctctgatct ggtcgatggt aatgtagcgc ggctcgattc catgagctct
      121 aaagaccccg gctatcgttc gtatgtaagg agagatatac ggcggtaccc caagacacgc
      181 aggttcatca acgtagccat cgatgatgaa tgcatcagca ttcatatccg atcaaccgga
      241 gaagacgccg ggcccacagt agttttcctc ttttcatgtg acccacgttc atgtcgtcga
      301 gatcaaatcc tacaaaaaca atccgttctg ctccgagggc atcggctgcg aacgctgcac
      361 ggtctccatc agagaaaccg ccaaaattat gaagaggggg gaatggctgc gactgggtgg
      421 taccgacaac cggcccgggg aaaaggggaa cccagtactg cagctgaggg atattatccc
      481 catgggcatg gatgaccata atcgttccct cgcgattcat cgcaagaaag gactctgacg
      541 ccccatccag gtcagtgaag actgcgttgg gccttattcc ggactcatac aggacaccag
      601 ctgctgcatc ggctgcgaag atgctccctt cgatatgttc tgtttcataa gggagggtag
      661 gggcatttcc gcatactgtg acgatccgtc cactgcaaag ggatgagagg aggctgcagt
      721 catccttccc aatcagggaa gcgaggatgc gggcggcctt ctcgtcatcc tcccttacga
      781 taccgaaata gtcaagaatc tcctgataat agggctccca ttcctcaaat tccatttctg
      841 acctcgcttt caagaccgac aatgtttgca aacctgccca ggagtggttc cgtgacaagg
      901 gagaggagat ccgccttgga tttggcgagg ctgaaaaatg agagggggat aacggccgcg
      961 gccatggtcg gatgcccccc tgcttccccc atcgtcccaa atgcctcctt gagtacgttg
     1021 ccgatatgga gccggacatc ccggttccgg gctgacatga tgatagcctc atctgagatg
     1081 ccgtagataa gcgccgtatt gacaccttca agcgttatca ggaggtcggc tgcctgggga
     1141 agtgcatccc ggttcctgat aaaaccgacg ttggagaaga gatacccgct ctggatcttc
     1201 ctgttcgcta tcgcgattcc aagaatatcg agcgtttctt cagagagcga gggtgacatg
     1261 atctggtcga gaagtgaatt gtcggtgagg gggaggagaa aggccgcgtt gatcaggtcc
     1321 tggggggtga cattccggga aaagtcgcgg gtatccgacc gaatcccgta gagaagaccg
     1381 gtggcaaccc gtttgtcaac accgatatcc agctcctgca gatactggga gaggatactg
     1441 gcggttgccc cgatgcccgg cctgctgtcg acaaatactg caggcgattc agcaagcttg
     1501 ccatcctcat ggtggtcgat cacgatatgc acccgcgtct ttgggggaag ggtattattc
     1561 acacccggtg cgggagaatc aacgagggca aggtaggtac attcctggag caccgcggga
     1621 tccatcttct ccatcttgat ctcaaggagg ttgacgagag tccggtttgc ctggtgcccg
     1681 attatcccat cataaaagat ccgacatacc aacctgttcg gatttgcatt tttcgcgatg
     1741 acagcaaggg cgagcgcact tgagatagca tcgggatcgg gatttttatg ggtgataatc
     1801 ccgagagtcc cctcccatga agagaggagg gaataaaggc gctgggatac acgattggca
     1861 tacaccttgt tgatctggta gaccgcagcc tttgccatca gctggttctg gttgaggaca
     1921 aaatctgctc ctgcctcttc cagatttcgc gcaacaaggg gatccgatgc ccgggccatg
     1981 attcgggaat gggggtacgt ttcccgaagc gctttgacag cagcaaggtt ggcttcgtta
     2041 tctccggtaa ggacaaacgc gatctcaatc gcaggaatgc ctgaaaggaa atccacagcg
     2101 aagatgtcac ggcgcacggc atcgatcctc tggtccctga gttccctgac acggtcctca
     2161 tttttatcaa tcacgagaac attccccgta ctgctctgca attcctgcag cacatggtat
     2221 cccgtgctgc cgcatcccag cacggcatac aatacccgtt ccctgctccc gttctgcgcc
     2281 gaatctgcca taacagatta gatgtaaacc cgacaggata taatggttac aattttattg
     2341 agatcttcag ggtttcggaa cacgttacat gctccgtttc ccaatcaggg caagaaatca
     2401 tgggaactcg aaaggtttat caatgaggca caaccaatgg tataggtcaa atccggggct
     2461 tgtagcttag ttcggaagag cgacggactc ttaatccgta ggtcaagggt tcaaatccct
     2521 tcaggcccgc tgttttcccg attttatcgt atctcgtgac gcacgaaccg cttactattc
     2581 gttgcaacag aaaaatgtct gaattcagga gttagaatca cctttttccc ccacctaaat
     2641 gttcatttaa catttcagtg agtttcttat ggatttcagt gatattccta ataagaagat
     2701 agaatcttct gcagaaacaa aaaccaggtt acaataacga tggaaaatca tccagcattc
     2761 acacagggat acattctgta tggagaacgt gttgtgaaat cactcttctg gcattgatat
     2821 ggctgctgat atacaaaaga gacattatgg gcattttcaa ccacattggc ctgatatatt
     2881 tcaaaaaggg cacaattttc ggtagatttt acagaacact cattttaaat aagggtgaag
     2941 caagatatta taagacatgc aggaagtgga tttcctgaac aaaccagagc attcactgga
     3001 ttcacaagca ggggatgttc tctttttttc aacagtacag aaaaaggtgg caataagcag
     3061 tccggtaaag gtgcttattc tcgatctggt aaaa
//
LOCUS       DAOQYR010000160         3077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_33834_length_3077_cov_7.556585, whole genome shotgun sequence.
ACCESSION   DAOQYR010000160 DAOQYR010000000
VERSION     DAOQYR010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33834_length_3077_cov_7.556585"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..792)
                     /locus_tag="PKM87_11825"
     CDS             complement(94..792)
                     /locus_tag="PKM87_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNQ30800.1"
                     /translation="MTILIAIDDTDTKESRGTGRLARTIADSLRHFGSVAGVTRHQLF
                     VHPSIPYTSHNSCAVIHLQTANGVAVPEIVDFVSGKILDDFIEGSDPGLAVAPTSGIG
                     DLVVKFGQDAKKCVLSRGDAVTLAERIGIALVGLGGSCDGVIGALAGLGLASSGNDGR
                     YVMKGRLRELSREARVEDLLLAGVDEVHTMAGERVKFGTVRMRKFPKPSLRNHRAVLF
                     VEERDGSFDEVVRD"
     gene            1168..1980
                     /locus_tag="PKM87_11830"
     CDS             1168..1980
                     /locus_tag="PKM87_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004198 - calcium-dependent cysteine-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C2 family cysteine protease"
                     /protein_id="HNQ30801.1"
                     /translation="MTATACSKTPYPEWNDPLLGVNYGNQNQGPCCFIASLSAIAWIW
                     PYNVEPFKCRSPFSTEADGTRKYKIPFKFPPENIEINEKVALEGSNYCFSRSMDAQEL
                     WVSLYEKAYARKLTQADMCDSYASVPWPGNAIDPLKHITGYSANSEIDSTKIYSKIQS
                     KCMSYKTKYATVVWSSSHAYSVLGYMPYNSTEYLVLRDPAGSNLYIRSDIVENVDWSI
                     PEDFVWNPFTNTKSNSPCRGGSLKIRLTGGIFGLKYDKVKDYFQGCAWAGPV"
     gene            2014..>3077
                     /locus_tag="PKM87_11835"
     CDS             2014..>3077
                     /locus_tag="PKM87_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30802.1"
                     /translation="MRMVHPGNLKNRNLTFLLVSLIFLVLLPGIAVAATNDTATCTIL
                     SVLTGDSHQCYPAIHDDKVIWIDSPGSGCAHLYNLSTGEECVLTTAFIPADGLCPDVS
                     CRYATWTTFDGSGYGVILHDMQTSEESWIAGGNGSTALNAKISSDCVVWESNALGRFD
                     IYQMDIRTGEVILLTPGTDDSDQTNAVVSGDWVAWQSLDPTSGTNDIYLASLLTGNVT
                     LVTPGTEDTDELYPAIDGDHLVYQRLDPANYCYDIYLYALSSKETVLLTPDTDSTSEE
                     YPAVHQGKVIWLGQDPGDFSYQLYIYETGSGLTYRLQRDFDTDSPGLPRIFGNRIVWQ
                     QPNPDTGYFDIFMMTLGVEE"
BASE COUNT          753 a          782 c          803 g          739 t
ORIGIN      
        1 agaaccgcct tcacgcccag aaagccctgc tcgtgttcct gatgcaggac ggggaataac
       61 ggggggccgg caggagaacc ggggcaattt tttttagtcc cttactacct cgtcgaagga
      121 gccgtcccgc tcctcaacga agaggactgc cctgtggttg cggaggctgg gctttggaaa
      181 tttcctcatt cggaccgtgc cgaacttcac gcgctcgccc gccatggtat gcacctcgtc
      241 cacacctgcc agaagaagat cttccactct tgcttcccgg gagagttcgc gaagcctccc
      301 tttcatcacg tacctgccat cgttcccgga cgaagccagg ccaagccctg cgagagcacc
      361 gatgacgcca tcgcagctcc cgccaagacc gaccagggca atccctatcc tctcggcaag
      421 ggtgactgcg tccccgcggg agagaacgca cttttttgcg tcctgcccga atttcaccac
      481 caggtcacca atcccggaag tgggtgccac cgcgaggccg gggtcgctcc cctcgataaa
      541 atcatcaagg atcttcccgc tcacaaaatc cacgatctcg ggaacagcga ctccgtttgc
      601 ggtctgaagg tgaatcaccg cacaactgtt atgcgaggta tatgggatag acgggtgcac
      661 gaagagctgg tgcctggtca cgcccgcaac tgaaccaaaa tggcggagtg aatcggcgat
      721 agtccgggcg agcctgcctg ttccacggct ctcctttgta tcagtgtcat cgatggcaat
      781 gagaatagtc atggaaggtc acgggaggta aagtaactgt actgtatgat cccgaaaagg
      841 gaataacatt gtgtagaagg ctgcacactc ttgagtggat tcgcctgtca cctggttgcc
      901 gatattgagc gaggtgtttg ttgttttttt taattattga agataatcct cgcataagct
      961 ttttaaataa gtgatatgag tatgatgtaa cgccaggagt acagcgttcg gtactcgtcc
     1021 agcttggggg ttgggggtgc acatggtttg cagtgggagg atggatgccc acattcagca
     1081 tcttgcggcg tctggttaat ctgttcgatt tggaggcttt tcatggcatg tgaatgtaag
     1141 aaagtatcag gtagcgaatt ctgtaagatg actgcaacag catgcagtaa aaccccgtat
     1201 cccgaatgga atgatccatt gctgggtgtc aattacggca atcagaacca gggcccatgc
     1261 tgcttcatcg catctctctc agcgattgcg tggatatggc cctacaatgt cgagccattc
     1321 aaatgcagat ccccctttag cacggaggca gatggaacaa ggaaatataa gatccccttc
     1381 aagtttcccc ccgaaaacat cgaaatcaac gaaaaggtgg cattagaggg ctccaattac
     1441 tgcttctcac gatcaatgga tgcgcaggag ttgtgggtgt ccctctatga gaaggcatac
     1501 gcacgaaaat tgacgcaggc ggacatgtgc gactcctacg caagtgttcc gtggcccggg
     1561 aatgcaattg atcccctgaa acatatcacg ggatattctg cgaatagtga aatcgactcg
     1621 accaaaatat attccaagat ccagtcaaag tgcatgagtt acaagaccaa atatgccacg
     1681 gttgtctgga gtagtagcca tgcatattca gttttgggtt atatgccgta taattcgact
     1741 gaatacctgg ttctgaggga tcccgcaggc tcaaatttat atatcaggtc tgatatcgtc
     1801 gaaaacgttg actggtcaat acctgaggat tttgtatgga atccctttac caataccaag
     1861 tcgaactctc cctgccgggg aggcagtttg aagataagac tcacgggagg aattttcggc
     1921 ctgaagtatg ataaagtcaa ggactatttc cagggctgtg catgggctgg cccggtgtga
     1981 tgccctgatg atccagtgag gaggacgtgg atcatgcgga tggtgcaccc tggtaacctg
     2041 aagaatcgga atcttacctt tctcctggta tcactcatat ttctcgttct tctaccaggg
     2101 attgcggttg ctgcaacgaa tgacaccgcc acctgcacta tcctctctgt gctcacaggg
     2161 gattcacacc agtgttaccc ggccatccat gatgacaagg ttatctggat cgatagccct
     2221 gggtcaggct gtgcccatct ctataatctc tcaacaggag aggagtgtgt tctcaccact
     2281 gcctttatcc cggcggacgg gctctgtccc gatgtatcct gcaggtacgc aacctggaca
     2341 acctttgacg gatcgggcta tggggtaatc ctccatgata tgcagacatc agaggaatcc
     2401 tggattgccg gcggaaacgg atccactgcc ctgaatgcca agatcagtag tgactgcgtg
     2461 gtatgggaat cgaatgctct cgggagattt gatatctacc agatggatat tcgcacaggc
     2521 gaagttatac tgctcacacc gggaacagat gacagtgacc agacaaatgc tgtagtttca
     2581 ggtgattggg tggcatggca gagcctggat cctacatcag gtacaaatga tatctacctc
     2641 gcatcactct tgacgggaaa tgtgacactc gttacccctg gaactgaaga caccgacgaa
     2701 ctctatccag cgatcgatgg cgatcatctt gtttaccagc gactggaccc ggccaactac
     2761 tgttacgata tttatctcta tgcactctct tctaaagaga ctgtgctcct gactcctgat
     2821 acagattcta caagcgagga gtatccggcg gttcatcaag ggaaggtgat atggctggga
     2881 caggaccccg gggacttctc gtatcaactc tatatttacg aaacagggtc tggtctgaca
     2941 taccggctcc agagggattt cgacacagac tcccccggtc tgccgcggat ctttggaaac
     3001 aggatcgtct ggcaacagcc caatcccgat accggatatt tcgacatttt tatgatgact
     3061 ctgggtgtcg aagagca
//
LOCUS       DAOQYR010000161         3023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_34704_length_3023_cov_8.064016, whole genome shotgun sequence.
ACCESSION   DAOQYR010000161 DAOQYR010000000
VERSION     DAOQYR010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34704_length_3023_cov_8.064016"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PKM87_11840"
     CDS             <1..214
                     /locus_tag="PKM87_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30803.1"
                     /translation="ATYLSKLPPRTLVMFHFTGDPIISLEDGLSLFEAAGQPKVWYQY
                     NGTVHGVYSDTYAVDLHNELKTMFGQ"
     gene            complement(481..735)
                     /locus_tag="PKM87_11845"
     CDS             complement(481..735)
                     /locus_tag="PKM87_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30804.1"
                     /translation="MDLSLYLQPLSIFIEAIICTIAIAISVVKKKMYGWFIALTFGIY
                     VVYDIVAFMDASLSPSVVAIIFLIATLSMLYVVWILYCEK"
     gene            complement(2310..3023)
                     /locus_tag="PKM87_11850"
     CDS             complement(2310..3023)
                     /locus_tag="PKM87_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNQ30805.1"
                     /translation="MELGISSYCLIGRPLAETLDTLSGITGLIEVMDEGPHLITDPAV
                     FESYSQDFVIHAPYHGMNIASLFEGIRRASVEVMIDCFAIAAEIGAPVVMHPGYHAWN
                     TEKEAADRQFRISLHQLQESAADHSLTFWFENMGELNYFHFRTPDDIPSLGDSGFCLD
                     CGHANLNHCLPAFLETDFSHMHIHDNDGRRDTHNAVGEGTIDFRPVMAALERTGATAV
                     LEVKDLEGVLRSREVLDRL"
BASE COUNT          796 a          764 c          703 g          760 t
ORIGIN      
        1 cgcgacctat ctgtcaaaac tcccgccacg gacactggtc atgttccatt tcaccggtga
       61 cccgattatc tccctcgaag atggcctctc cctctttgaa gcggctggcc agccgaaagt
      121 gtggtaccag tataatggaa ccgtgcacgg agtatacagc gacacctatg cagtggacct
      181 gcataacgag ttgaaaacca tgttcgggca ataggtcgaa atcggtcttt ctcatcggat
      241 ctccatacgg gatccctgaa ctcggtcccg gaaagcgatg agcaatccag ggaagaatgc
      301 ttatcatttc aaccggtccc cttcagaacc gagaaaccaa tctgccacct gataggagtt
      361 tagagagact caggcctcat ttatgaacga taaaaatcag gtagccatag agccaccagg
      421 attctcggaa ctgagtgagg taaaggaatg gaggagtgtt gcctgaaaaa gattcacctg
      481 ttacttttcg caatagagga tccagacaac atacagcatc gacagggtcg cgatcagaaa
      541 gataatggct acaacgctcg gtgagaggga ggcatccatg aatgcgacaa tatcgtatac
      601 gacataaata ccaaaggtga gggcaataaa ccacccgtac atcttcttct tcaccacgct
      661 gattgcaatt gcgatggtgc agatgatcgc ctcgatgaag atagagaggg gctggaggta
      721 gagggaaagg tccatagaga tgagagcacc gttgaaaaat aaaaaaatga tccctcagac
      781 tcagaggaaa tacggggatg tcgtacgcat cgatttcagg aagatcctcc aacaccccca
      841 tgtagtgact cctcacttat tctctgtggt ttatctgcta tcttactcat cctcgtgaag
      901 gcaacacggt tccccggttg atggttccat ggtctctcct cttctcaaga atataaaaaa
      961 aacatacatg aagttccaag acttcgtacc aaagcaatga acattttaca ctcaaaactc
     1021 aatttcaatt tgccagctgt atcgtctgaa gtttgcaaaa ttttcatgac aaaatcagga
     1081 gatgatgcat tctccctgat ttccatgctt catttcaggt aatgacagac tcctttgtct
     1141 gaactgaata gaagtgagaa tcagcatcta cagtggatat ttttcctggt ctcctggtta
     1201 gcggttctga agggagatca aacactttca aaacgggaaa taatgccaga atgaaggggc
     1261 cccaggagat acacaccggc acacgagttc agccccacag aaccgtctct ttggcacacc
     1321 ccggttttcc gctgttttaa ccgggatact tacggtaatg cggcatcatg ttcttcgaga
     1381 cctctgtcgg gcccagtcag ggaatgtctt tcccacccgt tcaccttcat gccgttgaga
     1441 taaacggatt cattcctctg ctgcatcggg tcttcccgaa catttgttca gcccagcgat
     1501 ctcatctttt gaccgccaga agatcgtcaa gctgtttaga tcctccaact ggtcaggatc
     1561 gggtcgctgc atgtgtccca agttttcttc cctgaggcat gaccccctat gcattgaagt
     1621 ggtaaataac tttctctttg atccacgcag tgggtacagt ggtacttcca gaaaaatttc
     1681 atgtagtccc tggttccacc gggaattact gggatgatcg ggagcagcga gccttttgat
     1741 tcgaacctgt gtctcctgaa gtcttgacgg agaaaggatt ttttcccaat atcctcgcgt
     1801 ttctgtgtcc ggatgctaat ccatcgcatt tgtccggaga gatctcagca aacaaaacct
     1861 ccgatgttaa caatttcgat caaaatactc attactccaa taattttgta atggaaaggg
     1921 gacagcggag ggtatcggaa aacaacctca acctatttct cattactatt acctcggatt
     1981 ggggcttatc gcaatcgttt aactattcag ggaatcgatc ctttgagaat cagcattaaa
     2041 aatatctccc tgtttgaaat gtccctcttg atcgggataa ccgtttattt tgtgtatatc
     2101 atggtacagg atattcaaaa acaacaccgc ctcattcgag aaattgaagc cctggataca
     2161 ggcctgcttt ctgttcgaag aattctgcct cccaatgggt gtcccaagcg atatgtttgg
     2221 cgagggatgt aaccgctgta acaaaggtca ctatatggta agaggattaa aagcgatctg
     2281 ctctatagga aaagactcct ttacgtcctt cagagccggt ccagtacttc cctgctcctc
     2341 aggactccct caaggtcctt tacctctagg acagcggttg cccccgttct ttccagtgct
     2401 gccatgaccg gacggaaatc gatagtgcct tctcctaccg cattatgggt gtccctcctg
     2461 ccatcattat catggatatg catatgcgag aagtcggtct caaggaatgc cggcaggcag
     2521 tggttcaggt tcgcatgccc gcagtcgagg caaaacccgg aatcgccaag cgacgggata
     2581 tcgtcagggg tcctgaaatg gaagtaattt aattccccca tgttctcgaa ccagaacgta
     2641 agcgagtgat cggcagcaga ttcctggagc tggtgcaggg atatcctgaa ctgccgatcc
     2701 gctgcctctt tttcagtgtt ccatgcatgg tacccgggat gcatcacgac cggtgccccg
     2761 atttccgctg caatggcaaa acagtcgatc atcacctcca cgctcgccct ccggatacct
     2821 tcaaagagac tcgcaatatt catcccatgg tagggggcgt ggatgacaaa atcctgcgaa
     2881 tagctctcaa agactgccgg gtcggtgatg agatgaggcc cctcgtccat cacctcgatc
     2941 aggccggtga tccctgaaag ggtatcgagg gtctctgcca gcgggcgacc gatcaggcaa
     3001 tagcttgaga tgccaagctc cat
//
LOCUS       DAOQYR010000162         2988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_35261_length_2988_cov_10.889192, whole genome shotgun
            sequence.
ACCESSION   DAOQYR010000162 DAOQYR010000000
VERSION     DAOQYR010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35261_length_2988_cov_10.889192"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..1233
                     /locus_tag="PKM87_11855"
     CDS             58..1233
                     /locus_tag="PKM87_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HNQ30806.1"
                     /translation="MGLFSGKPDQKKGDMVYAWTSDPEIEKKAECGGAVTTLLKFALE
                     HKMVDAVLAITKGQDIYDAVPTLIRDPKDLAKTAGSLHCGTLNTAKLVAKYLDGAKGM
                     KIGMTVKGCDLMALQELAKRKKVNLDQLLLIGVNCGGTVSPVLARKMIAEKYGVDPDT
                     VHKEEIDKGQFIIEYEGGHKGISIDELEEEGFGRRSNCRRCLYKVPRQADLACGNWGV
                     IGEKAGKATFVEVCSDKGADLLSRAVKTGALKTEAPNPKGLEIRGKVEGAMLKLGEKW
                     RKKDFAALSTDPWGIIKKETARCIKCYSCIENCPVCFPVEEQLKGKQYMVKPGEVPPN
                     PMFHLRRFAHISDSCVNCGQCEELCAMEIPLAKFSHAIRVEADAEFEPKLGKSTYTN"
     gene            1595..2983
                     /locus_tag="PKM87_11860"
     CDS             1595..2983
                     /locus_tag="PKM87_11860"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNQ30807.1"
                     /translation="MMVILCLALAFCGICGGESNTQPQFPHMFYGTATIMGSPAPEGY
                     TITTAVDGAANGMISVSPAGTYGGEGALDQKLIVQGDFQASTSRIRFAVEGSPALCRE
                     VGSGEGWQESYPFTPGAVTNLELNVGEVALLADFSVNRPDGYAPHTAQFTDQSTGSPD
                     QWVWNFGDGKTSPEQNPSHTYTTAGQYTVSLTVTSGTLTNVRTKSNYITVFAQGSPSG
                     GGGGGGGGGGGGSGTVITSAQTATPTTTGTLATTPTLTRISAGNLLLGTDNTTTREVT
                     IAATDTIGSLAIAKGVAPVDSTGAPLSTVSLSALPAGTIPPKSPCISAPYQYEITPAG
                     VRFTSGVTLVLSFNETTWAGLDKEGASLARYNTTDASWEEIATSMDPGSRTMKAAITR
                     GGTYVLCLDEGSQPMPTTEPPVTPASTGSPGLPLGIIALASLLIIVVVGVIAYLISTR
                     TTNDDSPPEGGQ"
BASE COUNT          713 a          832 c          870 g          573 t
ORIGIN      
        1 tcccggagtt caaggcctgt gctgtcaagg tcgagaagat tgcggaggcg aagtaacatg
       61 ggactcttta gcggtaaacc ggaccagaag aagggagaca tggtctatgc gtggaccagc
      121 gatcccgaaa ttgaaaagaa ggcggagtgc ggcggtgcgg tcaccaccct gctcaagttt
      181 gcccttgagc acaagatggt ggatgccgtc ctcgccatta ccaaagggca ggacatctat
      241 gatgccgtcc ctaccctgat cagggacccg aaagaccttg caaagacggc aggttccctc
      301 cactgcggga ccctgaatac cgccaagctt gtggcaaagt atctcgatgg tgccaagggc
      361 atgaagatcg ggatgaccgt caagggatgc gacctgatgg ccctgcagga gcttgccaag
      421 agaaagaagg tgaaccttga ccagctgctt ctcatcggag tgaactgcgg tgggacggtc
      481 agccctgtcc ttgcccggaa gatgatcgcc gagaagtacg gagtcgaccc tgacaccgtc
      541 cacaaggaag agatcgacaa ggggcagttc atcatcgagt acgagggagg ccacaagggg
      601 atctccatcg atgagctcga ggaggagggt tttggacgcc gctccaactg ccgccgctgc
      661 ctgtataagg tcccgcggca ggccgacctc gcctgcggga actggggggt catcggtgag
      721 aaagccggca aggccacctt cgtcgaggtc tgcagtgaca aaggggccga tctcctctcc
      781 cgggcagtga agacaggggc gctcaagacc gaggcaccga acccgaaggg gctggagatc
      841 cgcggaaaag tagaaggggc aatgctcaag ctcggggaga aatggcggaa gaaggacttt
      901 gctgccctct caaccgaccc ctggggtatc atcaagaagg agacagcgcg gtgtatcaag
      961 tgctactcct gcattgagaa ctgcccggtc tgtttccctg ttgaggagca gctgaagggg
     1021 aagcagtaca tggtaaaacc cggtgaagtc ccgccaaatc ccatgttcca tctgagacgc
     1081 tttgcccaca tctctgattc atgcgtcaac tgcgggcagt gcgaggagct ctgtgccatg
     1141 gagatcccgc tggccaagtt ctcccatgcc atcagggtgg aggcagatgc ggaattcgag
     1201 ccaaaactcg ggaaatccac ctacaccaac tgattcccta attttctttt ttcttttcag
     1261 aactacctaa gagaatccgg attccggaat catgatcagg taatggattg gacgagttct
     1321 tttttgattt tattccagaa aatgggagat tcttccaaaa aacttatgta tgaacgccat
     1381 cattccgtta tatataaaca ttacgtgcgt ggaaggattg gcataccccg ggaaagagat
     1441 cccctgacct gccgggtttg caatccggcc agcagaggtt gatagaagca tggtgcagga
     1501 gagaagtaac cagggacaat atcggaataa ggacgctcat aatccgatag gaagggatga
     1561 acaaaccagg gggattggca gaacggggca gtgcatgatg gtcatcctct gtctggctct
     1621 cgctttttgt ggtatctgtg gtggagagag caacacccag cctcagttcc cccatatgtt
     1681 ctacgggacg gcaacgatca tgggaagccc ggcacccgaa ggatatacca tcaccacggc
     1741 ggttgacggt gcggcgaatg gcatgatcag cgtatcccct gcaggaacat acgggggaga
     1801 aggtgcactg gaccagaagc tgatcgtcca gggggatttc caggcctcca cttccaggat
     1861 caggttcgca gtggagggat cgccggcact ctgtcgtgag gtcgggagcg gagagggatg
     1921 gcaggagagc taccccttta cgccgggtgc ggtgaccaac cttgaattga acgtcgggga
     1981 agtggccctc ctggctgatt tttcggtgaa tcggcctgac ggttatgccc cgcatacggc
     2041 gcagttcact gatcagagca ccggatcccc cgatcaatgg gtatggaatt tcggtgacgg
     2101 gaagacctcc cccgaacaga atccgagcca tacctacaca actgccggtc agtacacggt
     2161 atcactcacg gtcaccagcg gaacgctgac caatgtccgc accaagagca attatatcac
     2221 cgtatttgcc cagggcagcc ccagcggagg cgggggcggt ggtggaggcg gtggtggggg
     2281 tggcagcgga accgtcatca ccagcgctca gactgccact cccaccacaa ccgggaccct
     2341 ggcaacaacc ccgacgctca cccggattag tgctggaaac ctgctccttg gcaccgacaa
     2401 taccaccaca agggaggtta ccattgcagc taccgacacc atcgggtcac tggcgatcgc
     2461 gaagggggtg gctcctgtcg attccaccgg agccccgctc tcaaccgtct ctctctctgc
     2521 tctgccggcc ggtacaatcc cccccaaatc tccctgcatt tctgccccct atcaatacga
     2581 gattacccca gccggggtga ggtttacttc cggcgtcacc cttgtgctct ccttcaacga
     2641 gaccacatgg gcaggtctgg acaaagaggg agcttcactg gcaaggtaca atacaaccga
     2701 cgcttcatgg gaggaaattg ccaccagtat ggatcccggt tcacgtacga tgaaggcggc
     2761 aataacaaga gggggcacct atgtactctg tctcgatgag ggttcacaac ccatgcccac
     2821 cacggagcca cctgttactc ccgcatccac aggatccccg ggactccccc tgggcattat
     2881 cgccctggca agcctgctca tcatcgtggt cgtgggagtg atcgcatacc tgatcagcac
     2941 ccggacaacg aatgacgatt ctccaccgga aggtggccaa taacctcc
//
LOCUS       DAOQYR010000163         2941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_36056_length_2941_cov_6.880111, whole genome shotgun sequence.
ACCESSION   DAOQYR010000163 DAOQYR010000000
VERSION     DAOQYR010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36056_length_2941_cov_6.880111"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PKM87_11865"
     CDS             complement(<1..815)
                     /locus_tag="PKM87_11865"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNQ30808.1"
                     /translation="MKKRQILLSGLMAISLFMASAAATEYPLVGTWGSVDGSSFYDLY
                     ENGTGQISTRGEIYPCTWTNENSVFTITWSDSPVPEESTIDLVTLMDEGNSFAGMNNY
                     GGPVYAARITNLLFACLDEYTLQPNSALMVEGPGVLINDYQTNQDNLTAFLISSPLNG
                     DLVFNDDGSFTYTPSPEWWGTDTFTYLVKNETVTGTTAQVLLFVTKPENPSTFSISTS
                     RVGGGEISPSGIIPLQTGENQMFLVLGEKGSRLTSLTIDSITIDVVQDPPSPSA"
     gene            1056..1790
                     /locus_tag="PKM87_11870"
     CDS             1056..1790
                     /locus_tag="PKM87_11870"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNQ30809.1"
                     /translation="MRIVAFNGSPRAEQSNTHVMVSSFLAGAEDAGADTENIFLAKYT
                     INHCLGCFGCWLKTPGTCVQSDDMEELIKKYLSADIVIFATPLYIDNVSGIMKNFMDR
                     LIPMGDPHFEEDSNGEVRHVSGGKKDPKFVMMANSGFPEQTHFQVLRLLAQRMARNFD
                     TDLAGEIYRGGGSQLQEEELKPWIDAYKMQLRKAGWELVKEGKISLETTFRLELPLVQ
                     APGYVDMFIKNANEYMDQMIEINRKG"
     gene            complement(1856..2605)
                     /locus_tag="PKM87_11875"
     CDS             complement(1856..2605)
                     /locus_tag="PKM87_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ30810.1"
                     /translation="MNSAKPARRKRTGADLTSLGRYHEALKAFDEEMKDSGDLDLLLM
                     KALTLDRMGMFSDALKWCEQVIQKEPEHSDAWFVRGITLYWHAQLSDAARCLGHAVAL
                     DPDHGEAWLILGNCQYQIGDLDKALCSYDRVISLSPDHPNAWYNRGVCLADLERFEEA
                     LASYDTCIHINPRVSVVHANRGVALASLGRAEEALSAFDFALDLDPRDLTALNNKGLL
                     LSGLGRDEEAMVVLHMARELARYQSIPRRVW"
BASE COUNT          708 a          746 c          794 g          693 t
ORIGIN      
        1 gctgaggggc tgggagggtc ctggacgaca tcgatcgtaa tgctgtcaat ggttaaagag
       61 gtgagccggg atcctttctc cccaaggacc aggaacatct ggttctctcc ggtctggagg
      121 ggaatgatgc cagagggtga gatctcacca ccgccaacac gtgaggtgct gatcgaaaag
      181 gttgagggat tctctggttt tgtgacgaac agcagcacct gggcggtggt ccctgtcacg
      241 gtttcgttct tcaccaggta ggtgaaggtg tccgtccccc accattccgg ggacggggtg
      301 taggtaaatg atccatcatc gttgaagacc agatctccgt tcagggggga cgagatgaga
      361 aaggcggtga ggttgtcctg gttcgtttga taatcattga tcaggacccc tggcccttca
      421 accatgaggg ctgaattcgg ctggagcgta tactcatcca ggcatgcaaa caaaagattg
      481 gttatccttg cagcatagac cggtccaccg taattattca tcccggcaaa tgaattcccc
      541 tcatccatga gggtgaccag gtctatcgtg gactcttctg gcaccggact atccgaccat
      601 gtgatcgtaa agaccgagtt ctcattcgtc caggtgcagg ggtagatctc tcctcttgtg
      661 ctgatctgcc ctgttccatt ctcatacaag tcgtagaagg atgatccatc aacacttccc
      721 catgtgccaa ccagggggta ctccgtcgca gccgccgatg ccatgaacaa agaaatggcc
      781 atcaggccgg aaaggaggat ctgacgcttt ttcatgcagg aatatagacg agtaacaata
      841 tttcaatgtt acgctggatt taatcaaatc cgcctcacca gaatgtaaaa aaacgacttt
      901 tacggaaata gaacaggctt tctaccggta gccatcgtga atccaacaga cccagaaccc
      961 ccctcatatc tgcacgtcct atttgaatgg catactttta tgggatgtat catgaattcc
     1021 cctttcatac ggcatcacaa tggtgggggt aacagatgcg aattgttgct tttaacggga
     1081 gtccccgggc agaacagagt aatacccatg tcatggtatc gtcctttctt gccggggcag
     1141 aggatgcggg agcggacacg gagaacattt tccttgcgaa atatactatc aaccattgcc
     1201 ttggctgctt tggatgctgg ctgaaaacac cggggacatg tgtccagtct gacgatatgg
     1261 aggagttgat caaaaaatac ctctccgcag atatcgtcat ctttgcaaca ccgctctata
     1321 tcgacaacgt ctccgggatc atgaagaatt tcatggaccg gctcattccg atgggagatc
     1381 cccacttcga ggaagattca aacggggagg tccggcatgt ttcgggaggg aagaaggacc
     1441 cgaaatttgt catgatggcg aactccggct ttcccgaaca gacgcatttc caggttctcc
     1501 gcctccttgc tcaaaggatg gccaggaatt tcgacaccga tcttgctggg gagatttacc
     1561 ggggaggggg gtcccagctc caggaggaag agctgaaacc gtggatcgat gcctacaaga
     1621 tgcagctccg gaaagcagga tgggagttgg tgaaggaagg aaaaatttcc ttggagacaa
     1681 cgttccggct cgaactgcca ttagtccagg ctccaggata cgttgacatg tttatcaaaa
     1741 atgccaatga atacatggac caaatgatcg agattaacag gaaggggtag atctcagctt
     1801 tttttcttca tcggtcgcta tcttgtggag atgaggaaaa cctcctccct cccgttcacc
     1861 ataccctccg cgggatggac tggtatcggg ccagctcccg tgccatatgg aggacgacca
     1921 tcgcttcctc gtcccgtccg agccctgaga ggaggagtcc tttattattc aatgcggtga
     1981 gatcacgggg atcaaggtca agggcaaaat caaaggctga gagggcctct tctgccctgc
     2041 caagtgacgc aagggcgact ccccggtttg catggaccac actgacccgg ggattgatat
     2101 ggatgcaggt atcatacgat gccagggctt cttcgaaccg ctccagatcg gcaaggcaga
     2161 ccccccggtt ataccaggca ttcgggtgat cgggagagag ggagatgacc cgatcatagg
     2221 aacagagggc tttgtccagg tcaccaatct ggtactggca gttacccagg atcaaccagg
     2281 cctccccatg gtccgggtcg agtgctaccg catggccgag gcatcgggcc gcatcggata
     2341 gttgggcatg ccagtacagc gtgatgccac ggacgaacca ggcatcggaa tgttccggct
     2401 ccttttggat cacctgctcg caccacttca gtgcatccga gaacattccc atccggtcca
     2461 gggtgagcgc tttcattaaa agaagatcca gatcccctga atctttcatc tcttcatcaa
     2521 aagctttgag tgcttcgtga tatctcccca gagaggtgag gtctgctcct gttcgtttcc
     2581 ttctggccgg ttttgcgctg ttcactccac atactcctgt tcactatctt gatgcccttt
     2641 cacttgaaga cgatgttttg ggtcgataca gaggattacc ctcgtttcag acaaagaaga
     2701 tacgggttgg atattaatgc ctttttttcc gggcttgagg cctgaatgtc ccataacaca
     2761 gcctgcgagg atactacttg ccggaacttc gctgaacatc cgcagaaaca ggtttggtgc
     2821 gaccgggtgc aaaccaggtc gccttgtatg ttcactgttc agctgcccgc agggagggtg
     2881 agggatcttc agaaccactt gaacagcgag aaattcctca ccgggaacag gggggtgaag
     2941 a
//
LOCUS       DAOQYR010000164         2911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_36644_length_2911_cov_7.937325, whole genome shotgun sequence.
ACCESSION   DAOQYR010000164 DAOQYR010000000
VERSION     DAOQYR010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36644_length_2911_cov_7.937325"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2911)
                     /locus_tag="PKM87_11880"
     CDS             complement(<1..>2911)
                     /locus_tag="PKM87_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HNQ30811.1"
                     /translation="VISFGHDQSFWTLSTVPLSVTATVNAYFTYGGADNMRNLLRFIQ
                     GRVLSQTVTWEAPSLHFWEGVYHPDAPRVFATADEYWQWKGKDRSHTIGILFYRIYWA
                     NGDLHAIDALIRECEREYNAIAVFSVGVGDTEAGALDATEVIQGFLPDIDALICLQSS
                     ALSHDPDEPVRVFQALGVPVVHPLVLYYRTEQEWMESLDGMGSIELGWAVVLPEMYGM
                     TGMIPVASAMSEGPLGPEHAWHSPVEERVRTLVRRVGNLIRLRNKPDAEKRIAFVLNS
                     SACASVEANVGAAAHLDSLQSVVEILSRMKEKGFCVEVPESGEALAQAILERRAINEF
                     RWTTVQDIVERGGALGMVERETYEKWFSELPAPLQSRIAETWGDPPGEAKDGVPPAML
                     YGGKMVIPGLSFGNAVLCTQPKRGCAGSRCDGQVCRILHDPEVPPPHHYLAVYRYLGR
                     VFGADLIVHVGTHGTLEFLPGKSVALSAECLPDAILGDLPLLYIYNSDNPSEGTIAKR
                     RAGAVIIDHLQTVMAPTSPYGVLKELEEKIAEYHKFSGSDRARAHALQHQIIDLVARE
                     GLDRELGIQPIQHDPAAFDRLLEGAERLISGIYGTRIPEGMHVFGRIPAGPKRARFIA
                     PILNHDGHLHRLLAGMMGLDIRISPSETALLRVLDGYSEDLVSRLLEGEPPSQAASGV
                     LGDCLSMVDGEGLASLQREVQEISRALDYSDEIGSLISGTKGSYIPPGPSGLLSRGKT
                     EILPTGRNFYSLDPSSVPTEAAWEVGTRLAEILVERYRQEHGNYPENVALLWMASDIM
                     WADGEQCAQALALIGAEPVREHGRLKGFRIIPLERLGRPRIDLTIRVSGILRDCFFGC
                     VEFLDDAIRAVAALDEPSEWNYLRKHSVDNETGPRIFGAPRGTYGMGVNLAVYASAWN
                     NVSDLADVFIYWNGYSYGRGVFGEKSTEHLVSQLRTVDVTFNKTATDEYDLLGCC"
BASE COUNT          545 a          952 c          871 g          543 t
ORIGIN      
        1 cagcagcacc cgaggaggtc gtattcgtcg gtcgcagtct tgttgaaggt cacatcaacg
       61 gtccgcaact gggagaccag gtgttccgtc gacttctccc caaagactcc cctgccgtag
      121 gagtacccgt tccagtagat gaagacatcc gcaaggtcgc tcacattatt ccaggccgat
      181 gcgtagactg caaggttgac ccccatcccg tacgtccctc tgggtgcgcc gaagatcctt
      241 gggccggtct cattgtccac cgaatgcttt cgcaggtagt tccactccga cggctcgtcg
      301 agtgcggcca cagccctgat cgcatcatcc aggaactcga cacacccgaa gaagcagtcc
      361 cgtaaaatgc cgctcaccct gatcgtgaga tcgattctcg gcctgccgag ccgctcgagc
      421 gggatgatgc ggaaaccctt cagcctcccg tgctcccgga caggttcggc gccgatcagg
      481 gcgagggcct gggcacactg ctctccgtca gcccacatga tgtccgaggc catccagagg
      541 agggccacgt tctcggggta gttcccatgc tcctggcgat accgctcgac caggatctcc
      601 gcaagacgcg ttcctacctc ccatgccgcc tcggtcggga ccgatgaagg gtcaagggaa
      661 tagaagttcc tcccggtggg gaggatctcg gtctttcccc tggagaggag gccggaaggc
      721 cccggcggga tgtagctgcc cttcgttcca ctgatcaggc tcccgatctc gtccgagtaa
      781 tcaagggcac gggatatctc ctgcacttcc ctctgaaggg aggcaagtcc ctccccgtca
      841 accatgctca ggcaatcccc gagcacccca gaggccgcct gcgagggtgg ctctccttcg
      901 aggaggcggg agaccaggtc ctcggagtag ccgtcgagca cccgaaggag tgcagtctcg
      961 gaaggggaga tccggatatc gagccccatc atgccggcaa gcaaccggtg gagatgcccg
     1021 tcgtggttca ggattggcgc gatgaaccgg gcccgcttcg gaccggccgg gatgcgcccg
     1081 aacacgtgca tcccttccgg gatgcgggtg ccgtaaatac ccgagatcag ccgctcggcg
     1141 ccttccagca gacgatcgaa cgctgcgggg tcatgctgta tgggctggat acccagctca
     1201 cgatcaagcc cttccctggc aaccaggtca atgatctggt gctggagcgc gtgggccctt
     1261 gccctgtcgg accccgagaa cttgtggtac tcggcaatct tctcctcgag ttccttgagg
     1321 accccgtacg ggctggtggg ggccatcacc gtctggaggt ggtcgatgat gaccgccccg
     1381 gccctccgct ttgcgatggt gccttccgac gggttgtccg agttgtagat gtataggaga
     1441 gggaggtctc caagaattgc atcggggagg cactccgcag atagtgccac agacttcccg
     1501 ggaaggaact cgagcgtgcc gtgtgtcccg acgtgcacga tgaggtctgc cccgaagaca
     1561 cgcccgaggt agcggtagac agcaagataa tggtgaggag gagggacctc cgggtcatgg
     1621 aggatccggc agacctgccc gtcgcaccgg gaaccggcgc atcccctctt cggctgggtg
     1681 cagagcacag catttccaaa cgagaggccg ggtatcacca tcttcccacc ataaagcatc
     1741 gcgggcggga ctccgtcctt cgcctctccc ggcgggtccc cccatgtctc ggcgatgcgg
     1801 gattgcagcg gcgcagggag ttcggagaac cacttctcgt aagtctccct ctccaccatc
     1861 ccgagcgccc cgcccctctc cacgatgtcc tggaccgtgg tccaccggaa ctcgttgatg
     1921 gcacgccgtt caaggattgc ctgcgccagg gcctcgccac tctccgggac ttccacgcag
     1981 aaccccttct ccttcattcg cgacaggatc tcaaccacac tctggagcga gtccaggtgc
     2041 gctgcggccc ccacatttgc ctccaccgat gcgcaggcgg agctgttcag gacaaacgcg
     2101 atcctcttct ctgcgtctgg cttgttgcga agcctgatga ggttgcctac cctcctgacc
     2161 agggtccgga cccgctcctc caccggcgag tgccaggcat gctcggggcc aagcggcccc
     2221 tctgacatgg cgctcgcaac cgggatcatg ccggtcatcc cgtacatctc gggaagaacc
     2281 acggcccagc ccagttcgat gctgcccatg ccgtcaaggc tctccatcca ctcctgctcg
     2341 gtccggtaat acagcaccag cgggtggacg accgggacgc cgagcgcctg aaaaacccgg
     2401 accggctcgt cagggtcgtg ggagagggcg gaactctgga gacagatgag cgcatctata
     2461 tccggcagga acccctggat cacctcggtg gcatcgagtg cacccgcctc ggtatccccc
     2521 acccctaccg agaagacggc gatcgcattg tattcacgtt cgcattccct gatgagcgcg
     2581 tcgattgcat gcaggtcccc gtttgcccaa taaatccggt aaaagaggat gccgatggta
     2641 tgcgacctgt cttttccctt ccactgccag tactcatccg cggttgcgaa tacccgcggt
     2701 gcgtccgggt ggtacacccc ttcccagaag tggagcgagg gggcctccca tgtcactgtc
     2761 tgcgagagga ccctcccctg gataaacctc agcaggttcc tcatgttgtc cgccccgccg
     2821 taggtgaagt aggcgttcac cgttgcggtg acagagagcg ggacagtcga gagagtccag
     2881 aaggactggt cgtggccgaa cgatatgaca g
//
LOCUS       DAOQYR010000165         2881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_37226_length_2881_cov_9.047771, whole genome shotgun sequence.
ACCESSION   DAOQYR010000165 DAOQYR010000000
VERSION     DAOQYR010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37226_length_2881_cov_9.047771"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1277)
                     /gene="hisD"
                     /locus_tag="PKM87_11885"
     CDS             complement(24..1277)
                     /gene="hisD"
                     /locus_tag="PKM87_11885"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNQ30812.1"
                     /translation="MWKAVTLEDWAKNRVSELSSARPVVEEIISRVRNGGDRALLDLT
                     RQFDGVDVDSLLVGEDEREKAFQEVDERVIASLIEADARISRFHELQQPRGLWLEEVE
                     PGVVLGVKTTPLDRIGAYIPGGRAAYSSTALMCTIPARVAGVRSICCCSPPPISPLTL
                     VAMDIAGVDEIYAVGGAQAIAAMALGTESIRPVQKIVGPGNIYVTMAKMLLRDHAEID
                     FPAGPSEIVVLADDSARAEFIAADILAQSEHDPRAASLLVTTDPGLPARVGTCLESGM
                     KDAKRAVILEKAMENAGYLVAASMEEAVEIVNHAAAEHLSLQVADPMGVLHGIRCAGA
                     VFLGNYAAVACGDYASGTNHVLPTAGYAQVHSGLDVQHFCRRSTVQLITREGLEGMGD
                     TVETIAEAEGLVAHARSVRIRRELH"
     gene            1427..2398
                     /locus_tag="PKM87_11890"
     CDS             1427..2398
                     /locus_tag="PKM87_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNQ30813.1"
                     /translation="MHLNQRTAGILILLSALIISGAFFAGCTTTTPSQTTPVQTQPAE
                     RTKVLLATTTSLYDTGLLEYLKPMFDEKYNVDLLITSQGTGKAIEIATRGDCDILAVH
                     SPSQEQAFMDGGHGVNRRCFAYNYFIIVGPASDPAGIKGMKPEDAFKKLIDQGKAGTA
                     GVSFASRGDNSGTHSAEKNIWKAAGYNYTTDIQKSGNWYIEAGRGMGETLQLASEKQA
                     YTLTDEGTYLAYKGNLGLVPVIEQGDILLNVYSVITVYTPTQPSEKIAMANNFVNFLI
                     SPEIQTAIGEFGKEKYGKNLFTPMAGGCSQFKCDCTSPATATKPATA"
     gene            2542..>2881
                     /locus_tag="PKM87_11895"
     CDS             2542..>2881
                     /locus_tag="PKM87_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30814.1"
                     /translation="MRENIIPPARFIICIICILSFSLGACAATTSLHIIKYGNDGVTV
                     LKETTVSYEWMEKNLPVQGDGVTHYYHQGPVFLDDPNPEAQEMLRWNPPEDKNVQEKD
                     MGAVKGTNLRD"
BASE COUNT          676 a          842 c          769 g          594 t
ORIGIN      
        1 ttacaaaaat ggaggacccg ccttcaatgc aactctcttc ggatcctgac tgaccgggca
       61 tgggctacga ggccttctgc ctccgcaata gtctcgaccg tgtctcccat cccttcgagg
      121 ccttcccttg tgatcagctg gaccgtggac cgcctgcaga agtgctgcac atcaagtccc
      181 gaatggacct gtgcgtatcc cgcggtcggc aggacatggt tggttcctga cgcatagtca
      241 ccgcaagcga ccgcggcata gttaccaaga aacacagccc ctgcacacct gatgccgtgc
      301 aggactccca tgggatcggc gacctggagg gagaggtgtt cggcggcggc atggttcacg
      361 atctcgaccg cctcttccat ggatgccgcc acgaggtacc ctgcgttctc catggccttt
      421 tcaagtatga cggccctctt tgcatccttc atccctgatt cgaggcatgt tcccacccgg
      481 gcaggaaggc ctggatcggt agtcacgagg agacttgctg cccggggatc atgctctgac
      541 tgggcaagga tgtcggcggc aatgaactcc gcccgtgccg aatcgtctgc aagcaccacg
      601 atctcgctcg ggcccgcagg gaaatcgatc tccgcgtgat cccggagaag catctttgcc
      661 atcgtcacgt agatattccc gggcccaaca atcttctgga cgggccggat actctctgtc
      721 ccgagcgcca tggccgcgat tgcctgcgca ccgccgaccg cgtagatctc gtcgacaccc
      781 gcaatgtcca tcgccacaag ggtcaggggg gatatggggg gcgggctgca gcagcagatg
      841 gatctcacgc ctgcaaccct cgcaggaatc gtgcacatca gtgcggtgga tgagtaagcc
      901 gcccttcctc ccgggatgta ggcaccgatg cggtcgaggg gggtggtttt gaccccgagc
      961 accacgcctg gctcaacttc ctcgagccac aatcccctcg gttgctgaag ttcgtggaaa
     1021 cgggatatcc gtgcgtccgc ctcgatcaga cttgcgatga cccgctcatc cacctcctgg
     1081 aaggccttct cccgttcgtc ctccccgacc aggagagaat ccacgtcaac tccatcgaac
     1141 tgcctggtga gatcgagcag tgcccggtca ccaccgttcc tgacccgtga gatgatctcc
     1201 tctaccacag gcctggccga ggagagttcc gagacccggt tttttgccca atcctcaagc
     1261 gttacggcct tccacatggg gaaaacttcg actgggggaa tgttaaaggt ttttttcgcc
     1321 catatcacct gatatataca gctgataatg caagtggtta acaataacgt caatgttaat
     1381 ggaaatattc atcaggaatc ctgtataagg actagccgtg agacctatgc atctcaatca
     1441 aaggacagca ggtattctta ttttactcag tgccctcatc atcagcgggg cattctttgc
     1501 aggttgcact acgaccaccc cctctcagac aaccccggtt cagacccagc cggctgaaag
     1561 gacaaaagtg ctgctggcca ccaccaccag cctctatgac accgggcttc tcgaatactt
     1621 aaagcccatg ttcgatgaga aatacaatgt cgatcttctc atcacttcgc aggggacggg
     1681 aaaagctatc gagatcgcta cacgtggtga ctgcgacatc ctggccgtgc attctccgag
     1741 ccaggagcag gcattcatgg acggcggaca tggggtcaac cgtcgctgct ttgcgtataa
     1801 ttactttata atcgtgggac ctgcaagcga tccagcaggc atcaagggta tgaagcccga
     1861 ggatgcgttc aagaaactga tagaccaggg gaaggctggg actgccggtg tctcgtttgc
     1921 ctcccgcggg gataactccg gaacccattc cgcagagaag aatatctgga aggccgcagg
     1981 gtataattac accactgaca tccagaagtc cgggaactgg tacatcgagg ccgggcgggg
     2041 catgggagag acgctgcagc ttgcatcaga gaagcaggcc tacaccctga cggacgaggg
     2101 gacctacctt gcctacaagg ggaaccttgg actggtccct gtcatcgaac agggggatat
     2161 cctcctgaac gtctacagtg tcatcacggt gtatacacct acccagccat ctgaaaagat
     2221 tgcgatggcc aacaactttg tgaatttcct catctcccct gagatccaga cagccattgg
     2281 cgaattcggg aaggaaaagt acggcaagaa cctctttacc ccgatggcag ggggatgcag
     2341 ccagttcaag tgcgactgca cgagcccggc aactgccacg aagcctgcga ctgcctgacc
     2401 attcctattt tttccatgac tggataaata cagggaaagg tgggagtaaa atccccggtc
     2461 ggctaccggg cagaaggccg taacttttga gatcaactgg attgtaaaca catgaaacct
     2521 aacccgaatt gtgagagaaa aatgagagag aacatcatac cacctgcaag attcatcatc
     2581 tgcatcatct gcatcctttc gttctccctt ggagcctgcg ccgccaccac atccctgcac
     2641 atcatcaaat atggaaatga tggggtcaca gttctgaaag agacaactgt ctcgtatgag
     2701 tggatggaaa agaatctccc ggtgcagggc gatggtgtca cccactacta ccaccagggc
     2761 ccggtcttcc ttgacgaccc taatccagag gcccaggaaa tgctgcgatg gaaccctccc
     2821 gaagacaaaa acgtccagga aaaggacatg ggtgcggtga aggggaccaa tctccgtgac
     2881 c
//
LOCUS       DAOQYR010000166         2707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_41006_length_2707_cov_6.826546, whole genome shotgun sequence.
ACCESSION   DAOQYR010000166 DAOQYR010000000
VERSION     DAOQYR010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41006_length_2707_cov_6.826546"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..922)
                     /gene="cbiQ"
                     /locus_tag="PKM87_11900"
     CDS             complement(95..922)
                     /gene="cbiQ"
                     /locus_tag="PKM87_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HNQ30815.1"
                     /translation="MIEDLFFLEKQTYKKSLVHSLDARVKIIIAFAAIIAIVAVPYST
                     VIYTVGAVFFIFFAVMWAACGLSPVVYLKRLVVILPFGIFLIVFQIFFKNRYYESFTT
                     IATLPFGIEVYAESVQFASILLVKFLVSVSFIILLSSTTRTQDLLEGAGRLGLPAEFT
                     LTLGMMLRYLYVFGYMIRKMTQSLETRCFDPFDRALSYRYRLEKVAYAVGTLFIRSYE
                     QGERTYMAMLCRGYGRESHLYISRKPLQRRDWIFLSTGMVLVILTPVLAWTHFITLV"
     gene            complement(919..1752)
                     /locus_tag="PKM87_11905"
     CDS             complement(919..1752)
                     /locus_tag="PKM87_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ30816.1"
                     /translation="MHLLETRDLTYVYPGNVTGLDHVNFIAGRNARIAVIGANGAGKS
                     TLFKHFNGILRPTSGTVLVHGEPVTQKNIREVRKFVGLVFQNADDQIFSPTVEQDVAF
                     GPMNLGLDADTVSHRVEEALAMVGIEHLKERVPHHLSGGEKKRVAIAGVIAMEPQVIV
                     LDEPTAGLDPKGVKDLISVINALAREYGITVIFSTHDVSLVPEVADYIYVMHRGSFVA
                     QGSVSTVFADLELLCSVRLDVPVIPKLIRQLREEGLDIGMAYTLDEAKTAFLKSYRER
                     A"
     gene            complement(1795..2145)
                     /locus_tag="PKM87_11910"
     CDS             complement(1795..2145)
                     /locus_tag="PKM87_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HNQ30817.1"
                     /translation="MMDKKTFIFAGIVIALIIAAMAPFIASSNPDGLESAFFGIFGAK
                     ELQGSSLDEEMAGSAEEEVAGITGNVFSFDSPFPDYSISGLAKFGEAAAVIFGTLLVV
                     LVAWGLGKVISRPH"
     gene            complement(2142..>2707)
                     /gene="cbiM"
                     /locus_tag="PKM87_11915"
     CDS             complement(2142..>2707)
                     /gene="cbiM"
                     /locus_tag="PKM87_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HNQ30818.1"
                     /translation="LALRWARRELSEEKIPLVAILAAGIFAIQAVNIPIPWGTSGHMM
                     GAALAAIVLGSPFAAVFILTLVLIVQGVLFGDGGLTVMGANIINMGVIGGFVGYYSYR
                     AFYPIFKSPYAPAFIAGWLSLFIAALACAVEMALAGTFPLGLGLFSMGMYHAVIGIIE
                     GGITASAIYLIATARPDIMANTEGVPA"
BASE COUNT          652 a          735 c          784 g          536 t
ORIGIN      
        1 tgcagatgag cacgatggtc gtgacaaatc cccgtccgaa tttactctcc atgaaggagg
       61 tatcaggcat cgcaggcgat aaaggatgcg gaagttatac cagggtaata aaatgggtcc
      121 aggcaaggac aggagtgaga atgacaagca ccatcccggt ggacaaaaat atccagtccc
      181 ttctctggag gggttttctt gaaatataca ggtggctctc ccgcccgtac ccgcggcaga
      241 gcatggccat gtaggttcgt tcgccctgct cgtaggaccg gatgaagagg gtccctacgg
      301 catacgccac cttctccagg cgatagcggt aggagagagc ccggtcgaag gggtcgaagc
      361 agcgggtctc gagcgactgg gtcatctttc ggatcatgta cccgaagaca tagaggtagc
      421 ggagcatcat cccgagcgtg agggtaaact cggcagggag cccgagccgc ccggctccct
      481 cgagcaggtc ctgtgtccgc gtggtggagg agaggaggat gatgaacgag acggagacga
      541 ggaacttgac aaggaggatg gaggcaaact ggaccgattc ggcatacacc tcgatcccga
      601 acggcagggt cgcgatcgtc gtaaaactct cgtagtagcg attcttgaaa aaaatctgga
      661 acacgattaa aaaaatcccg aacggcagga taacgaccag ccgtttgaga tagacgactg
      721 gcgagagtcc gcatgccgcc cacatgacgg caaagaatat gaagaacacc gcaccgacgg
      781 tatatatcac ggtcgagtag gggacggcaa cgatcgcgat gatggctgcg aatgcgatga
      841 tgatcttgac ccgtgcatca agggaatgga cgaggctctt tttgtaggtc tgcttctcga
      901 ggaagaagag gtcctcgatc atgcccgttc cctgtaggac ttcaggaagg ccgtctttgc
      961 ttcatcaagc gtataggcca tcccgatatc gagcccctct tcacggagct gcctgatcag
     1021 cttcgggata accggcacgt ccagccggac cgagcataag agctccaggt cggcaaagac
     1081 agtggagaca ctgccctgtg cgacaaagct tccccggtgc atcacgtaaa tatagtcagc
     1141 aacttccggg acaagggaga catcatgggt cgagaagatc acggtgatcc cgtactctct
     1201 tgcgagagca ttgatcaccg agatcaggtc ctttacccct tttggatcga gtccggcagt
     1261 tggttcgtcg aggacgatca cctgcggttc catcgcgatc acccctgcga tagccacccg
     1321 tttcttctct cctccagaca ggtggtgggg aaccctctct ttcaggtgct ctatcccgac
     1381 catggcaagg gcttcctcga cacggtggct gacggtatcc gcatccagcc cgaggttcat
     1441 gggcccgaat gccacgtcct gctcaacagt gggagagaag atctggtcgt cggcgttctg
     1501 aaatacgagc ccgacaaatt tccggacttc cctgatattt ttctgcgtga ccggctcacc
     1561 atggacgaga acggtcccgc ttgtcggacg caggatcccg ttgaagtgtt tgaagagggt
     1621 gctctttccc gctccgtttg cgcctatcac tgcaattctc gcatttctcc cggcgatgaa
     1681 gttcacatgg tcgagcccgg tgacgttacc ggggtagaca taggtcagat cgcgggtttc
     1741 aaggagatgc attgcaggaa tacttcgcga aaaaaggata aatagtctct tttttcaatg
     1801 aggcctggag atcaccttgc ccagtcccca ggcaacaagc accacgagca gtgtgccgaa
     1861 gataacggca gcggcttctc cgaatttcgc aagcccgctg atgctgtaat cagggaacgg
     1921 cgaatcgaat gagaatacat taccggtaat cccggccact tcctcttctg ctgaaccggc
     1981 catctcctca tcgagggacg atccctgcag ctcctttgcg ccgaagatcc cgaagaaggc
     2041 actctccaac ccatcgggat tgctggaggc gatgaagggt gccatggccg caatgatcag
     2101 ggcgatgaca ataccggcaa aaatgaaggt tttcttatcc atcatgccgg caccccttcg
     2161 gtattcgcca tgatatcagg gcgggccgta gcaatgagat agatcgcaga tgcggttatc
     2221 ccgccttcaa tgatcccgat cacggcatgg tacatgccca tggagaagag gccaagcccc
     2281 agggggaagg ttcctgcgag tgccatctcc accgcacagg ccagggctgc aatgaaaagg
     2341 gagagccacc cagcgatgaa tgcaggtgca tacggggatt tgaagatcgg gtaaaaagcg
     2401 cggtagctgt aatacccgac aaaaccgccg ataaccccca tgttgatgat gtttgccccc
     2461 atcacagtga ggccgccatc accgaagaga acgccctgca cgatgaggac gagtgtcagg
     2521 atgaacactg cagcaaaagg ggatccgagc actatggccg ccagggctgc gcccatcatg
     2581 tgcccgcttg tcccccaggg gatggggatg ttgaccgcct ggatggcgaa aatgccggca
     2641 gcgaggatgg caacaagcgg gatcttctct tccgagagtt cccttcttgc ccacctgagg
     2701 gcgaggg
//
LOCUS       DAOQYR010000167         2707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_41009_length_2707_cov_4.569382, whole genome shotgun sequence.
ACCESSION   DAOQYR010000167 DAOQYR010000000
VERSION     DAOQYR010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41009_length_2707_cov_4.569382"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..922)
                     /gene="cbiQ"
                     /locus_tag="PKM87_11920"
     CDS             complement(95..922)
                     /gene="cbiQ"
                     /locus_tag="PKM87_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HNQ30819.1"
                     /translation="MIEDLFFLEKQAYKKSPVHALDARVKIIIAFAAIIAIVAVPYST
                     VIYTVGAIFFAFFIVLWAASGLSPTVYFKRLAVILPFGIFLIVFQIFFKNPYYESFTT
                     IATLPFGIEIYAESVQFATILLVKFLVSVSFIILLSSTTRTQDLLEGAGRLGLPAEFT
                     LTLGMMLRYLYVFGYMIRKMTQSLETRCFDPFDRTLPYRYRLEKVAYAVGTLFIRSYE
                     QGERTYTAMLCRGYGRESHLYIAKKPLKGRDWTFLAAGLLLIIATPLLAWIGIITLA"
     gene            complement(919..1752)
                     /locus_tag="PKM87_11925"
     CDS             complement(919..1752)
                     /locus_tag="PKM87_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ30820.1"
                     /translation="MHLLETRDLTYVYPGNVTGLDHVNFIAGRNARIAVIGANGAGKS
                     TLFKHFNGILRPTSGTVLVHGEPITQKNIREVRKFVGLVFQNADDQIFSPTVEQDVAF
                     GPMNLGLDAETVNHRVDEALAMVGIEHLRERVPHHLSGGEKKRVAIAGVIAMEPQIIV
                     LDEPTAGLDPKGVKDLIGVINALAREYGITVIFSTHDVSLVPEVADHLYVMHKGSIVA
                     EGSVSDVFADLELLSSVRLDVPVIPKLIRQLSEEGLDIAMAYTLDEAKAAFLKTYRER
                     A"
     gene            complement(1795..2145)
                     /locus_tag="PKM87_11930"
     CDS             complement(1795..2145)
                     /locus_tag="PKM87_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HNQ30821.1"
                     /translation="MMDKKIFILAGVAIALVIAAMAPFLASSNPDGLESAFFGIFGAK
                     DLQGSTLDEEKAGIAEEEVTAITGNEYSFDSPFPDYSIEGLAKFGEAAAVIIGTLVVV
                     GIAFGLSRVVARKG"
     gene            complement(2142..>2707)
                     /gene="cbiM"
                     /locus_tag="PKM87_11935"
     CDS             complement(2142..>2707)
                     /gene="cbiM"
                     /locus_tag="PKM87_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HNQ30822.1"
                     /translation="LALRWARRELSEEKIPLVAILAAGIFAIQAVNIPIPWGTSGHMM
                     GAALAAIVLGSPFAAVFILTLVLIVQGVLFGDGGLTVMGANIINMGVIGGFVGYYSYR
                     GLLPVFKSPYAPAFIAGWLSLFVAALACAVEMAVAGTFPLELGLLSMGLYHAVIGIIE
                     GGITAFAVYLIATARPDIMADATVVPA"
BASE COUNT          601 a          740 c          841 g          525 t
ORIGIN      
        1 tgcagatgag cacgatggtc gtgacaaatc cccgtccgaa tttactctcc atgaaagaga
       61 tatcaggtcc agggagggat aaagggtgcg ggagtcacgc gagggtaata atcccgatcc
      121 atgcgaggag gggggtggcg atgatcagca gcaatcctgc cgcgagaaat gtccagtccc
      181 tccctttcag gggttttttc gcgatgtaca ggtggctctc ccgtccgtat ccgcggcaga
      241 gcatggctgt gtaggtccgc tcgccctgct cgtaggaccg gatgaagagg gtccctacgg
      301 catatgccac cttctccagc cggtaccggt aggggagggt tcggtcaaag gggtcaaagc
      361 agcgggtttc gagggactgg gtcatcttcc gtatcatgta cccgaagaca tagaggtaac
      421 ggagcatcat gccgagcgtg agggtgaact cggcagggag cccgagccgt ccggctccct
      481 cgaggaggtc ctgggtccgc gtggtggagg agaggaggat gatgaaggag acggagacga
      541 ggaacttgac aaggaggata gtggcaaact ggaccgattc ggcatagatc tctatcccga
      601 acggcagggt ggcgatcgtg gtaaaactct cgtagtatgg gttcttgaaa aagatctgga
      661 atacgatcag gaagatgcca aacggcagga tgaccgcgag ccgtttgaag tagaccgtgg
      721 gggagagccc cgatgcagcc cacagcacga taaagaatgc aaagaagatc gcaccgactg
      781 tgtagatcac ggtggagtac ggtacggcaa cgatcgcgat gatggccgcg aaagcgatga
      841 tgatcttgac ccgggcatcg agcgcatgga ccgggctctt tttatacgcc tgcttctcga
      901 ggaagaagag gtcctctatc atgcccgttc cctgtaggtc ttcaggaagg cagcctttgc
      961 ttcgtcgagg gtatacgcca tcgcgatatc gagcccttcc tcgctgagct gccggatcag
     1021 cttggggatg accgggacat ccagccggac cgagcttaag agttccaggt cggcaaagac
     1081 atccgagacg ctgccctcgg caacgatgct ccccttgtgc atcacgtaga ggtggtcggc
     1141 aacctcgggg accagggaga catcatgggt ggagaagatg acggtgatcc catactcacg
     1201 agccagggca ttaatcaccc cgatcaggtc ttttactccc ttcgggtcga gcccggcggt
     1261 cggctcatca aggacgatga tctggggctc cattgcgatc accccggcaa tcgccaccct
     1321 cttcttctct ccaccggaaa ggtggtgggg gacccgctcc ctcaagtgct cgatccctac
     1381 catggccagg gcttcatcga cacggtgatt gaccgtctcg gcatccaggc cgaggttcat
     1441 cggcccgaag gccacgtcct gttcgaccgt cggagagaat atctggtcat cggcgttctg
     1501 gaagacaagc ccgacaaact tcctgacttc ccggatgttt ttctgggtga tcggttcgcc
     1561 atggaccagt actgtgccgc tcgttggccg gaggatcccg ttgaagtgct tgaagagggt
     1621 gctcttcccc gcaccatttg cgcctatcac cgcgatccgg gcatttctcc cggcgatgaa
     1681 gtttacgtgg tcaagcccgg tcacgttccc ggggtagacg taggtcagat cgcgggtttc
     1741 aaggaggtgc attgcaggaa tactgtgcga aaaaaataga taaatggttg tctttcatcc
     1801 tttgcgggcg acgacccggg ataaaccgaa ggcgatgccg acaacaacca gggtgccgat
     1861 gataaccgca gcagcctcac caaacttcgc gagtccttcg atactgtaat cagggaaggg
     1921 tgaatcgaat gagtactcgt tgccggtgat cgcggtcacc tcctcttctg ctatccctgc
     1981 cttctcctca tcaagtgtcg agccctgcag atcctttgcc ccgaagatcc caaagaaggc
     2041 actctcgagg ccgtcaggat tgctggaggc aaggaacgga gccatcgcgg cgatgaccag
     2101 ggcaatggca acgccggcaa ggatgaagat cttcttgtcc atcatgccgg caccaccgtt
     2161 gcgtccgcca tgatatcggg gcgggccgtg gctatcaggt agacggcaaa tgccgtgatg
     2221 ccaccttcga tgatgccgat gacggcatgg tagagtccca tggagaggag tccgagttca
     2281 agcgggaagg tccctgccac ggccatctcc accgcacagg ccagggctgc aacaaagaga
     2341 gagagccagc cggcgatgaa tgcgggggca tagggggact tgaagaccgg taagagcccg
     2401 cggtagctgt aatacccgac aaatcctccg atcactccca tattgatgat attggcaccc
     2461 atgacggtaa ggcccccgtc tccgaagagc accccctgca cgatcaggac gagggtgagg
     2521 atgaagacgg ctgcgaaggg tgagccgagg acgatagcgg cgagcgcggc acccatcatg
     2581 tgcccgctcg taccccaggg gatcgggata ttgactgcct ggatggcaaa gatcccggcg
     2641 gcaaggatgg ccacaagcgg gatcttctct tccgagagtt cccttcttgc ccacctgagg
     2701 gcgaggg
//
LOCUS       DAOQYR010000168         2702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_41141_length_2702_cov_6.684549, whole genome shotgun sequence.
ACCESSION   DAOQYR010000168 DAOQYR010000000
VERSION     DAOQYR010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41141_length_2702_cov_6.684549"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..95
                     /locus_tag="PKM87_11940"
     CDS             <1..95
                     /locus_tag="PKM87_11940"
                     /inference="COORDINATES: protein motif:HMM:NF014009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HNQ30823.1"
                     /translation="RLALGTWQGIYFCEFDGPRHRHVVIGVTVE"
     gene            complement(209..691)
                     /locus_tag="PKM87_11945"
     CDS             complement(209..691)
                     /locus_tag="PKM87_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30824.1"
                     /translation="MKRPKIVKTGLDSGIEALAERADHWTLATWAADCAERVLPFFEE
                     RSTGDPRPRDAIGALREWIRTGVFHMADVRKASLSAHAAARSVEEDDAARSAARAAGQ
                     AMASAHVPRHAIAAARYAATAVWYAAAGVDADAAAIQEREWQLHHLEDLVKGRDPDNP
                     "
     gene            1027..1572
                     /locus_tag="PKM87_11950"
     CDS             1027..1572
                     /locus_tag="PKM87_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30825.1"
                     /translation="MRLLDEESIQSSASTVGFVFPVHALTIPIAVRRFLRKAKFPSAE
                     YLSAVATRQGRTFCGFPLIDRLLAGHGKRLDAHSILNMGNNESRREGYQGPDGTRAPG
                     HSKRRRLRNCMSSWRRQHTKKPLREQDTTCTIPVGPFTMGRVMAGMTLFGVYRGRSVL
                     LRRLELHGVAGFAGRCVSLGI"
     gene            1823..2155
                     /locus_tag="PKM87_11955"
     CDS             1823..2155
                     /locus_tag="PKM87_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30826.1"
                     /translation="MPGDSHDRYVGLVVGDIGVGIVVWLAGGSGSGGVRGVARVEIGG
                     TGVGRDAVAAGDGVAGAGTEGSTSVVAGSTGGTGITMLTGGGEGGVDEIDGEVAGVII
                     VPEGVAFA"
     gene            2195..2539
                     /locus_tag="PKM87_11960"
     CDS             2195..2539
                     /locus_tag="PKM87_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30827.1"
                     /translation="MADGPPPWPEPDEAFPGGVSGWAASGPVVAGTCVDKAVPAVARS
                     FSDPAGGFPAGEVHPAHAKKKSSITTRTRYFITRRPRYSSVKSFWIKPLTTIIRQLPG
                     FCVRGNDAHSIS"
BASE COUNT          557 a          781 c          829 g          535 t
ORIGIN      
        1 gacgccttgc gctcggcacc tggcagggta tttacttctg cgagttcgac gggccacggc
       61 atcgccacgt agtaatcgga gtcactgttg aatagcacgc gagggagccc tgcagttctg
      121 ccatggaatg gataataaaa aacacaagac aaacgtctga aagagtgacg attactgaga
      181 ctgggtgagg aggatccctt tctcccaatc agggattgtc tggatctctc cctttcacca
      241 gatcctccag atgatgcaac tgccattccc gctcttggat agcggcggca tctgcatcga
      301 cgccagccgc ggcgtaccac accgcggttg cagcatacct cgccgcggcg atcgcatgac
      361 ggggcacatg ggccgatgcc atcgcctggc ccgcggcacg ggcggcggaa cgtgccgcat
      421 cgtcctcttc aacgctccgg gcggccgcgt gggctgagag ggaggccttc cggacatcgg
      481 ccatgtggaa cacgccggtg cgtatccatt cccggagtgc cccgattgca tcccggggac
      541 ggggatcacc cgtggaccgt tcttcgaaga agggtagcac ccgctcggca cagtcggctg
      601 cccaggtcgc gagcgtccaa tggtcggccc tttccgcgag cgcctcgatc cctgaatcaa
      661 ggcccgtctt cacaattttt ggtctcttca tcctggtctt ttaatcctcc cgcgggcccc
      721 tatgcgaaca cacgaaaaac aaaaaaagcc ctggcagcgg agggtttctt atcccttcag
      781 gatagtgtaa cacccagcga acctgtcatg gcgtaatgag tgtgtacgaa aacctccatg
      841 tgagtgatgc cccctttttt cttccattcc acggtattca tgagggattg gcggcgatta
      901 ccataaagag gaagggtgac ggggggatga gcacgcagat ctactccttc ccggggaccg
      961 ggaactcact cttcatcgcc cggcaactcg gagcgaagcc tccctggctc ccggctgatc
     1021 ccgattatgc gcctgcttga cgaggaatcc atccaatcca gtgccagcac ggtcggtttt
     1081 gtcttccctg tccatgccct cacgattcca atcgcggtga ggcggtttct ccggaaggcg
     1141 aaattcccct cggcagagta cctgtccgcc gtcgccaccc gccagggaag gacgttctgt
     1201 ggcttcccgc tgattgaccg tctcctggca gggcatggta agcggctgga tgcgcactcc
     1261 atcctcaaca tgggcaacaa cgaatcccgg cgggaggggt accaaggtcc cgacggaacc
     1321 cgagctcccg ggcactcgaa gaggcggcgc ttgcgaaact gtatgtcatc gtggaggcgg
     1381 cagcacacaa aaaaacctct ccgtgaacag gatacgacgt gcaccatccc ggtcggtccc
     1441 ttcaccatgg ggcgggtcat ggccgggatg acgctcttcg gagtatatcg ggggcgttca
     1501 gtacttctcc gcagactcga actgcacggg gttgcgggat ttgcgggaag gtgtgtctct
     1561 ctgggaatat aaggatggag ggggagcggc ccgcctggca gaaggacgtc ttctgctaca
     1621 tgtgcgttgc atgcatctca cttcttcccc cggaaggccg ttcagatcag gaacatcccc
     1681 ggcctgaagt cgtgcacatc ggtgaacgcg aggtacctac acccctacgc gaaaatcgaa
     1741 gatatcgccg cacagaaggg tttcagacaa taatcgtgtg ctctgctgag agtcagactc
     1801 cccggcgttt tatatatccg gggtgccagg agacagtcac gaccgctatg tcggcctcgt
     1861 cgttggtgat atcggggtgg gaatcgtggt ctggttggca ggggggagcg ggagcggcgg
     1921 ggtgaggggt gtggcgaggg tggagattgg ggggacaggg gtcggcagag atgcggtcgc
     1981 ggcaggggac ggagtggcag gagccggcac cgaaggaagc accagtgtcg tcgcaggcag
     2041 caccggggga acaggaatca cgatgctcac gggaggagga gaggggggcg tggatgaaat
     2101 tgatggcgag gtggcggggg taattatcgt ccctgaagga gttgcgttcg cgtgagcggg
     2161 tgtagcggga gtggaggttc cgggtgctat accggtggct gatggtcctc ctccctggcc
     2221 agaaccagat gaggcgtttc ctgggggagt ctcaggctgg gcggcgagcg gccccgttgt
     2281 cgcggggacc tgcgtggaca aagccgtccc cgcggtcgca cggtcattct ccgatccggc
     2341 agggggcttt ccagccggcg aggtgcaccc tgcacacgca aagaagaaga gcagtatcac
     2401 cacaaggaca agatacttca ttacaaggcg acctcgttat tcaagtgtaa aaagtttctg
     2461 gataaagcca ctcaccacca tcatccggca actccccggt ttttgtgtcc gcggtaacga
     2521 tgcacacagc atctcctgac cattcccgat cccgttcaca aatgagcatc tggatacatc
     2581 ctccggttcc agcctgaagc tcccggcacg catgattccc tcctttccat caatacacat
     2641 aaatccgcaa ggcacaacat gccagtcaac aacactattt tccagtgacc cgcatctgct
     2701 cc
//
LOCUS       DAOQYR010000169         2593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_43763_length_2593_cov_8.262017, whole genome shotgun sequence.
ACCESSION   DAOQYR010000169 DAOQYR010000000
VERSION     DAOQYR010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43763_length_2593_cov_8.262017"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..1098)
                     /locus_tag="PKM87_11965"
     CDS             complement(175..1098)
                     /locus_tag="PKM87_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HNQ30828.1"
                     /translation="MDILSAVLIIAGLILFETITSIDNAIINAEVLSTMKEWARRWFL
                     VWGLFIAVFVVRGVLPWLIVWMSTPALGPVGALTATFSSDPAVIAAIEESAPMLLMGG
                     GTFLVFLFFHWLFLEPKFYGIRGERYIATKGVWFFAVVSILLSLLVWEALQVNPMMAF
                     GAVVGSTAFFIVHGFRQNAEEQEKKMMGGNMSDISKIFYLEVIDATFSVDGVLGAFAF
                     TLSVPLIIIGNGIGAFVVRELTVRNVENIKKYRYLKNGAMYSIFVLGIIMILDSFGAH
                     IPNWLSPVVTFGIVGFFFYKSQRQIALKSRQ"
     gene            complement(1263..2438)
                     /locus_tag="PKM87_11970"
     CDS             complement(1263..2438)
                     /locus_tag="PKM87_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HNQ30829.1"
                     /translation="MSLFGGKPSVKKGDMFYAWTNDPDIAKKAECGGAVTSLLKYALE
                     NKIVDAVLAVKRGQDVYDAVPTLVTDPKQLKDTAGSLHCGSLLIAKPAVKILERNKGM
                     KIGMTVKGCDVMALHEYAKRKKIDMNNLLLIGVNCGGTVSPVMARKMIREKFGVDPDT
                     VHKEEIDRGQFIIEFEGGHKGIKIDDLEDEGYGRRTNCRRCVYKVPRQADLACGNWGV
                     IGDKAGKATFVEVCSEKGADLFSKAAKDGAISTQSPDPKGLEMRGKVEGAMIKLGEKW
                     RKKDFDALSKDLWGAIQKETSRCIKCYSCIENCPVCYPSADSLKTKQYMVKPGEVPPN
                     PMFHMRRFAHISDSCVNCGQCEELCAMDIPLAKFSHAIRVEADSAFEPKLGKSTYSN"
     gene            complement(2439..>2593)
                     /locus_tag="PKM87_11975"
     CDS             complement(2439..>2593)
                     /locus_tag="PKM87_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNQ30830.1"
                     /translation="KDIMKGVMFMPFHFKECAANVLTNNALDPIAKIPEFKACAVKVE
                     KIAEAK"
BASE COUNT          542 a          769 c          709 g          573 t
ORIGIN      
        1 atgcaattca cgaggcaaac gtccttgaca tgcagaacat cgggataggc atcgtggaat
       61 gtgcggaagt gccacgcgta ttgccccgat gaccaggaaa ggagctgtac gcggagttgg
      121 cttttgctcc cggaatcttt cttccggttg ctccatggtg ttcaacacgt taccttactg
      181 ccgtgacttg agagcgatct gtctttggga tttgtagaag aagaacccga ctatcccgaa
      241 ggtgacgact ggagagagcc agttcgggat gtgcgccccg aaactgtcca ggatcatgat
      301 gatgccaaga acaaagatgg agtacatcgc accgttcttg aggtagcggt atttcttgat
      361 attctccacg ttccggaccg tgagctccct caccacgaag gccccgatcc cgttcccgat
      421 aataatgagc ggcaccgaga gggtgaacgc gaatgccccc agcaccccgt cgaccgagaa
      481 ggtggcatcg atcacttcga gataaaagat cttgctgata tcggacatgt tcccgcccat
      541 catcttcttc tcctgctcct cggcgttctg gcggaagcca tggacgatga agaatgcggt
      601 cgaacccacg accgccccga acgccatcat gggattcacc tggagtgcct cccagaccag
      661 gagcgagagg aggatggaga ccacggcaaa gaaccagacc ccctttgtgg cgatgtagcg
      721 ctccccccgg atcccgtaga acttcggttc caggaagagc cagtggaaga agaggaagac
      781 gaggaacgtc ccacctccca tcaggagcat cggtgccgac tcctcgatgg cagcaatcac
      841 cgcgggatcg ctgctgaacg tcgcggtgag cgcccccacg ggtccgagcg cgggcgtgga
      901 catccagaca ataagccagg ggaggacgcc gcggaccacg aagaccgcga tgaacagtcc
      961 ccagaccagg aaccaccgcc tcgcccactc cttcatggtg gagagcacct cggcattgat
     1021 gatggcgttg tcaatgctcg tgatggtctc aaagaggatg agcccggcga tgatcaggac
     1081 cgcggagagg atgtccattg gtgtatgcga cacgagcgtt gtctaccaat ggtcaaatat
     1141 cttacgtcat ttcccagtaa aatcaaaaaa aatcccggat atctgcaatg ttcccggcag
     1201 cacatcaccc tgccggcctt ttcagttccg atactaaaaa accgaaaaga aaaatggggg
     1261 aatcagttgg aatatgttga tttccccagt ttcggctcga atgccgagtc tgcctcgacc
     1321 cgtattgcat gggagaactt cgcgagcggg atgtccattg cacagagctc ctcgcactgc
     1381 ccgcaattga cgcacgagtc agagatgtgg gcgaaccggc gcatgtggaa catggggttc
     1441 ggcgggactt ctccgggctt taccatgtac tgcttggtct tcagcgaatc ggccgatggg
     1501 tagcagaccg ggcagttctc gatgcacgag tagcacttga tgcaccggga ggtctctttc
     1561 tggattgcac cccagaggtc ctttgagagc gcatcgaagt ccttcttacg ccacttctcg
     1621 ccgagcttga tcatcgcccc ctcaactttt cctcgcatct cgagcccctt cgggtcaggc
     1681 gactgggtgc tgatcgcacc atcttttgct gccttcgaga agaggtccgc acccttctca
     1741 ctgcagacct cgacgaaggt tgccttcccg gccttgtcgc cgatgacacc ccagtttcca
     1801 cacgcaaggt ctgcctggcg cgggaccttg tagacgcagc ggcggcagtt ggtccgcctg
     1861 ccatacccct cgtcctcgag gtcgtcaatc ttgatgccct tgtggccgcc ctcaaactcg
     1921 atgatgaact ggccgcggtc gatctcctcc ttgtggaccg tgtccgggtc gaccccgaac
     1981 ttctccctga tcatcttccg ggccatgacc gggctcaccg tgcctccgca attcacgccg
     2041 atgaggagga ggttgttcat atcgatcttc ttccgctttg cgtactcgtg gagggccatg
     2101 acgtcgcaac ccttcaccgt catcccaatc ttcattccct tgttgcgctc gagaatcttc
     2161 acggcaggtt ttgcgataag gagtgacccg cagtgcagcg atccggcggt gtccttaagc
     2221 tgtttcgggt cggtgacgag ggtcggcacc gcgtcataca catcctggcc tctcttcacc
     2281 gcaaggaccg catcgacaat cttattctcg agtgcatact tgagcaggct ggtcaccgca
     2341 ccgccgcact ctgctttctt ggcgatatcc gggtcattgg tccatgcgta gaacatgtcc
     2401 cctttcttca cgctgggttt tccgccaaat agtgacatct atttcgcctc cgcaatcttc
     2461 tcgaccttga cagcacaggc cttgaactcc gggatcttgg cgatggggtc gagcgcgttg
     2521 ttggtaagga cgtttgctgc gcactccttg aagtggaacg gcatgaacat gacgcccttc
     2581 atgatgtcct tcg
//
LOCUS       DAOQYR010000170         2531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_45486_length_2531_cov_10.456381, whole genome shotgun
            sequence.
ACCESSION   DAOQYR010000170 DAOQYR010000000
VERSION     DAOQYR010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45486_length_2531_cov_10.456381"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PKM87_11980"
     CDS             complement(<1..362)
                     /locus_tag="PKM87_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNQ30831.1"
                     /translation="MKWTQVEVPTSARTQFLDITGAVSSEVAKSGVKTGICYVYMPHT
                     TAGLTINENADPDVARDILAGLARLVPATGDYRHAEGNSDAHIKASLVGFTVMVPVID
                     GRLALGTWQGIYFCEFDGP"
     gene            complement(359..1159)
                     /locus_tag="PKM87_11985"
     CDS             complement(359..1159)
                     /locus_tag="PKM87_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNQ30832.1"
                     /translation="MKVLVIMGSPRKGNTYRAAERIRVIMKETAQVDWEYVMLRDLHL
                     EQCRGCANCIVKGEEFCPVKDDALCLTQKMLDADGVIFASPVYALQVTGLMKVFIDRH
                     SYIMHRPRFFGKKALILSTAGAVGISDVLKYLESVVHMWGFEVAGKAGTFEEFIPGTR
                     QQENDQTLRAAAEAFHAALRRKSPPKPRCMDIIVFHGLRATFDELGDVFPADRAYWEE
                     KGWMEKDACYFVDVPVNPVYHAVGVMLEKYQRRQMRKDSRDTNHGVST"
     gene            1529..2233
                     /locus_tag="PKM87_11990"
     CDS             1529..2233
                     /locus_tag="PKM87_11990"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HNQ30833.1"
                     /translation="MTFIYRKNAVISWTGGKDGCFACLKAMLEGYQVTHLLHFTNVKK
                     RGSHELNPGIVRAQSESMGIPLVQKDFFSYEEEFMKAIRDLRAKGERIDGAVFGHIET
                     HKNLVDRICGELDLELVMPLWKQDSKRLLREMIASGLEIVVVSARGDLMGEEWLGRRI
                     DGEFIEDLESLDASIDHCGENGEFHTLVTDSPLFGKKIHITGASRIFQNGYWYLDIHE
                     WTLQEKGDSCRPGREK"
BASE COUNT          560 a          678 c          730 g          563 t
ORIGIN      
        1 ggcccgtcga actcgcagaa gtaaataccc tgccaggtgc cgagcgcaag gcgtccatcg
       61 atgaccggga ccatgacagt gaagcccacc agggatgcct tgatgtgggc atcggaattc
      121 ccctcggcat gccggtaatc gcctgtcgcc gggaccagcc gcgcgaggcc ggcgaggatg
      181 tcgcgggcta catcggggtc cgcgttctcg ttgatggtga ggccggccgt cgtatgcggc
      241 atatacacgt aacagatccc ggttttgacg ccgctcttcg ccacctccga cgaaacggca
      301 cctgtgatgt ccaggaactg ggtcctcgcg ctggtcggaa cctcaacctg tgtccacttc
      361 atgtactcac cccgtgattc gtatcccgtg aatccttccg catctgcctt cgctggtact
      421 tctcaagcat gacgcccacg gcatggtaca ccgggttgac cgggacatcc acaaagtagc
      481 atgcgtcctt ttccatccat cctttctctt cccagtacgc acggtcggcg ggaaacacgt
      541 cacccagctc gtcaaacgtc gcacggagac cgtgaaagac gatgatgtcc atacacctgg
      601 gttttggagg ggacttcctc cggagcgccg catggaacgc ctctgcggcc gcccggagcg
      661 tctggtcgtt ctcctgttgc cgggtgccgg ggatgaattc ctcgaacgtc cccgcttttc
      721 cggccacttc gaacccccac atgtgcacca ctgattccag gtatttcagc acgtcgctga
      781 ttccaaccgc cccggcggtg gaaagaatca gcgctttttt cccgaagaac cgggggcggt
      841 gcatgatgta ggaatgccgg tcgatgaaga ccttcatcag ccctgtcacc tgcagtgcat
      901 agacgggcga tgcgaagatc accccgtcgg catcgagcat cttctgtgtc agacacagag
      961 catcgtcctt taccgggcag aactcctcgc ccttcacgat gcagttcgca cacccccggc
     1021 actgttcgag gtggaggtcc cgcagcatga cgtattccca gtcgacttgg gccgtctcct
     1081 tcattattac ccggatgcgt tccgcggcac ggtacgtgtt tcctttccgg gggctgccca
     1141 tgataacgag gactttcatc tatatggtga gatgtgtggg gagcctggaa tataatatgt
     1201 gaggaatatc tccggacagg ttgctcatga atgaagtttg ttgttctgat tcagggcagt
     1261 catgatcgca tggatgcatt tttggaaacg aacggaatcc aggttgcact accttaggca
     1321 taatcccatg gaaaaggcaa ggaggtatac gatcatggga aaagaccgcg ataaaaaaga
     1381 ccagaatcta tcccggttca tatcacaatt cttatgccct ttctcaatgg agtgtgaagg
     1441 aaaaggcaat gcaggatagg catcaaggcg tggctaatcg aggtattcca gattctcctg
     1501 caggaatcgc atggagatgg actatactat gacgttcatt tacaggaaga atgcagtaat
     1561 ctcctggacg ggaggaaagg acgggtgttt tgcgtgcttg aaggcgatgt tggaggggta
     1621 ccaggtcacc catctcctgc actttacgaa tgtaaagaag aggggatccc acgagttgaa
     1681 cccgggcatc gtcagggcgc agtccgagtc catggggatc cccctcgtgc agaaggactt
     1741 cttctcttac gaggaggagt tcatgaaggc tatccgggat ctgagggcta agggtgagcg
     1801 gatcgatggt gcggtattcg ggcacatcga gacccacaag aaccttgtcg accggatctg
     1861 cggggagctg gatctcgagc tggtcatgcc gctctggaag caggattcaa aaagactctt
     1921 gagggagatg atcgcatccg ggcttgagat cgtggtggtc agcgcgagag gggacctgat
     1981 gggggaggag tggctcggcc ggaggatcga cggggagttc atcgaggacc ttgagagcct
     2041 agatgcctcc atcgatcact gcggggagaa tggggaattc cacacccttg tcaccgactc
     2101 gccgttgttt gggaagaaga ttcatatcac cggggcaagc cgcatattcc aaaatggcta
     2161 ctggtacctc gacatccacg agtggacgct gcaggaaaaa ggagattcat gtcgacctgg
     2221 acgggagaag taagacgatc tgcatcgctt ctctgtccgg gatcggcgat gtttgagtgc
     2281 atggaaaaaa ccggtgggag gttgatcttt ttttagacca gggctattct cttttccata
     2341 agggcatatg ctgaacagtg catgctcttt aatagcgaga aggtgcaact tccccgagat
     2401 ccgaacttga cagacacatg caaccttttt ctcaaatcca ggagcctgcc cggcctcctc
     2461 ctgttcattg ccggggcagt tctcctgatg ggcatcatca ccgcggagat attctatccg
     2521 gccggataca c
//
LOCUS       DAOQYR010000171         2502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_46293_length_2502_cov_7.706988, whole genome shotgun sequence.
ACCESSION   DAOQYR010000171 DAOQYR010000000
VERSION     DAOQYR010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46293_length_2502_cov_7.706988"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1368)
                     /locus_tag="PKM87_11995"
     CDS             complement(124..1368)
                     /locus_tag="PKM87_11995"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNQ30834.1"
                     /translation="MRSPPDANLRVIAFLALLLLVAAMPAAASVPSVIITDFEVTPSV
                     LLPGEQGIISVTVRNTADKATLTQSYLSDTTETTTNIDINAPIDSVTMVGNGIQVLSE
                     GYYRVGDIGPGQSIPLSFLIKAPAESGIYFPEIWIRVSDARSLKYPIPVNVNSEVAIQ
                     KRPQISIEKELPASVTPGNDFNATILLQNTGQVSASNLKITVNSSTPSITSTTPGTFH
                     IDRLGQGERQEIAMVFSTDRNAPLGLRPVTLLMEYQEPDGTFQQQVETLGVNIRGKAQ
                     VDISQITTDPATIQKGDQFTLIIRIENTGTDDARSVNAKIDIPLSGTKEAFVGKIEPG
                     NDAPAPFNLQADQSGDIPYTLVIQYEDDYGPGQESEDLHLNVYGNNNTALIIGVAVLL
                     LVVGIIGYWYFIKRRKPGTGNV"
     gene            complement(1832..>2502)
                     /locus_tag="PKM87_12000"
     CDS             complement(1832..>2502)
                     /locus_tag="PKM87_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30835.1"
                     /translation="WRCLGKRLYLIRTLPYERKYNPCWKYPDLKKFSKVVHVAHSCAR
                     IRYEFIRETADDKKRDSLPSLPSHTGLKKCSNVVRLARCRARILYEFIRGTADDKKKD
                     SLPSLQSEVACIVVPSGKEPWEVHYNAACFYALALNILTTYPHGAEQRTCIQSYATTA
                     IRHLEQVIRDRNYSSRTWIFDETNGDPDLKTLRKCHEFTLFALIGNPNPMGVEREDHT
                     KRME"
BASE COUNT          549 a          654 c          705 g          594 t
ORIGIN      
        1 atggagggga ggaagatcag gttcacaaat accatcccga taatgaggat cgtgaggtac
       61 agggtacctt tgtttccccg gacaatcgac cgcttcgcaa ggaagaaggc gacctttgac
      121 tcctcaaaca ttgccggttc ccggtttacg acgtttgatg aaataccagt acccgatgat
      181 tccgacaacg agaaggagga ccgccacccc gatgatcaga gccgtgttgt tgttcccgta
      241 tacgttcagg tgcagatcct cgctctcctg gcccggtccg taatcatcct cgtactggat
      301 caccagggta tacgggatat ctccggactg atcggcctgc aggttgaacg gtgccggggc
      361 atcgttgccg ggttcaatct ttccgacaaa tgcctctttt gtgcccgaga gcgggatgtc
      421 gatcttcgca ttcaccgacc gtgcatcatc ggtgccggtg ttctcgatcc tgatgatcag
      481 ggtgaactgg tcccccttct ggatcgtggc cggatccgtg gtgatctggg agatatccac
      541 ctgggctttg ccccggatat tcacgccaag cgtctcgacc tgctgctgga acgtcccatc
      601 gggttcctgg tattccatca ggagggtgac cggccggagt ccgagcggcg cattccggtc
      661 ggtggagaac accatggcga tctcctggcg ctccccctgg cccagccggt cgatatggaa
      721 ggtgccggga gtcgttgaag tgatggaggg cgtggaggag ttcacggtaa ttttcaggtt
      781 gcttgctgac acctggccgg tattctggag gaggatcgtc gcattgaagt cattgccggg
      841 cgtgacgctg gcaggcagct ctttctcgat gctgatctgg ggtcgcttct ggatcgccac
      901 ctcgctgttc acgttcaccg ggatcggata tttcaggctc cgggcatccg agacccggat
      961 ccatatctcg ggaaagtaga tcccgctctc tgccggcgcc ttgatgagga aggagagggg
     1021 gatcgactgc cccggaccga tatcgccaac ccggtaatac ccttcgctca gcacctggat
     1081 cccgtttccc accatcgtga cgctgtcaat cggggcattg atgtcaatat tcgtggtggt
     1141 ctcggtcgta tcagagaggt aggactgggt gagggttgcc ttgtcagcag tattcctgac
     1201 cgtcacgctg atgatcccct gctcgccggg caggaggacc gaaggagtaa cctcaaaatc
     1261 cgtgatgata actgagggaa cgctggctgc tgccggcatt gccgcgacga gaaggagcag
     1321 tgccaggaat gcaatgacac gcaggttcgc atccgggggt gatctcatga atgagtctct
     1381 gccagagctg gtcaataata ctatgcaaag gacatactct gaaggtgaac ctggaccatg
     1441 cagcaagaac cgcatgcaca ccgttcacgg caaggacagg agaatcccca agagtggcat
     1501 gtgtcgcttc ggtcagcagg tgtcccgctt ttgaaaggga acaggttgat gtttcgactg
     1561 aggggaggac cccctcccac ttcccatcgg caaaacagaa gcggactcat ggctttcacc
     1621 ccgcgatagt gatggagtcc ttgataaggg ttgcttatga aacggagaac aaaggaggga
     1681 attcccccca gaaaaaaagg gtgagaaatg tccgatgtat ccagggggag gcgaaatccg
     1741 cacccgataa ggatcccgat ggcagagacc ccatttcacg gataccgcca tcattccgtg
     1801 aaattccgag tgagcctctg catctcgata atcattccat acgttttgtg tgatcttccc
     1861 tctctacccc catcgggttt ggattcccta tcaatgcaaa gagggtaaac tcgtgacact
     1921 tccggagtgt tttgaggtcc ggatcgccat ttgtctcgtc aaaaatccag gtccttgatg
     1981 agtaattccg gtcacggatt acctgttcga gatggcgtat cgcagtcgtt gcatatgact
     2041 gtatgcacgt tctttgttca gccccgtggg gataagtcgt aagaatattc agggcaagag
     2101 catagaaaca tgcggcgtta taatgcactt cccagggttc cttacctgac ggtactacaa
     2161 tacacgctac ctccgattgt aaggacggta aggaatcctt ttttttgtca tcggcggtgc
     2221 ccctgatgaa ctcatagagg atcctggcgc gacatcgagc cagtcgtact acattggagc
     2281 attttttcag gcctgtatgc gatggtaagg agggtaagga atcccgtttt ttgtcgtcgg
     2341 ctgtctccct gatgaactca tatcggatcc tggcgcagga atgagccaca tgcactacct
     2401 tggagaattt tttcaggtct ggatacttcc agcagggatt atatttccgc tcatagggaa
     2461 gcgtacgaat cagatataat ctctttccca ggcatctcca cc
//
LOCUS       DAOQYR010000172         2209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_56200_length_2209_cov_8.996286, whole genome shotgun sequence.
ACCESSION   DAOQYR010000172 DAOQYR010000000
VERSION     DAOQYR010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56200_length_2209_cov_8.996286"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..427
                     /locus_tag="PKM87_12005"
     CDS             86..427
                     /locus_tag="PKM87_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30836.1"
                     /translation="MPDRRNTPVFLVISLVLIGIVVLPAVSAEGLAGPWISSSTSISG
                     NAVVNESASLKMVTYTGSIPVKQEWKFQNTFSPVQGNTWRSDLFFRPTENTLTFQYSR
                     LGLFSPVYLRV"
     gene            557..1927
                     /locus_tag="PKM87_12010"
     CDS             557..1927
                     /locus_tag="PKM87_12010"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNQ30837.1"
                     /translation="MIPGERICALNALPARSGEYVLYWMQSSQRTVGNLALEYAIRKA
                     NKFKIPVVACFCIDPSYPEANLRHYTFMAEGLVSAGRSLETMHIPLAIFWGVPGESVA
                     GAAKDAAMVVTDRGYLALHQTWRKDLAGMVDCPLVMVEDNAVVPVETVSGKEEWSAGT
                     IRKKIHRQLMRFLEPVGRTPVQYSTPSQEHGGTPYHEAHTLLTRVKEKNPGPSPLYSG
                     GEHEALLRLEKFITGDLSRYVSGQNDPASPVTSELSPYIHFGQVSPAYIAQRVLESRH
                     PGADAFLEQLIIRRELSLNFVHFNPHYDRYEGLPEWAKKTLAEHADDPREYVYTEAEF
                     EEARTHDPFWNAAQQELLVTGKMHGYMRMYWGKKILEWTENPEQAYAIALSLNNRYEL
                     DGRDPNGYAGVAWCFGKHDRAWGERPVLGKVRYMNAAGLKRKFRIEHYVERVAALAEE
                     HGPGAS"
     gene            1908..>2209
                     /locus_tag="PKM87_12015"
     CDS             1908..>2209
                     /locus_tag="PKM87_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNQ30838.1"
                     /translation="MARAPPDEPGEVLSLREVAEFQEKVYRFYRVHRRDLPWRNTHDA
                     YSILVSEMMLQQTQVERVLERYPRFIARFPDFSALAKASFPEVLGEWQGLGYNRRA"
BASE COUNT          482 a          598 c          666 g          463 t
ORIGIN      
        1 tgagaggaaa gcatataggt gaaagggtcc gatactagtg cgaacatgtt ccgttgtgcg
       61 ccgccacgcc acacggagga taatcatgcc agacagaaga aatacgcccg tattcctggt
      121 tatcagcctc gttttaatcg ggattgtggt cctgccagca gtctctgcag aggggcttgc
      181 cgggccatgg atctcgagca gcaccagtat ctcgggcaat gcagtcgtga acgaatctgc
      241 gagcctcaag atggtcacgt acacaggcag tatccctgtg aagcaggaat ggaagttcca
      301 gaacactttc tcccctgtgc agggaaatac ctggagatcg gatctcttct tcaggccgac
      361 agagaacacg ctgacgttcc agtactcgag gttggggctt ttctcccccg tatacctcag
      421 ggtataattt ttttctcctc gcggatcacc cctgtgactg gcaggatcca tctccatgac
      481 tcttgatatt tcagtgcgcc tgcaggatgc cggaagagaa ggttccatgt aggtgggaat
      541 accattgtcc tgatgcatga tccccggcga gcgcatatgc gccctcaacg cgcttcctgc
      601 gcgttcggga gaatatgtcc tctactggat gcagtcctcg cagcggacgg tgggaaacct
      661 tgcccttgag tatgccataa ggaaggcaaa caaattcaaa atacccgtcg tcgcctgctt
      721 ctgcatcgat ccctcttatc cggaggcaaa tctccgccat tatacgttca tggcagaagg
      781 cctggtttcg gcaggaagat ccctggaaac gatgcacatc cctctcgcga tcttctgggg
      841 agtgcccggg gagagtgtcg caggtgctgc aaaagacgct gcgatggtcg tgaccgaccg
      901 gggatacctc gccctgcacc agacctggag gaaggatctg gcggggatgg tggactgtcc
      961 gcttgtgatg gtggaggaca acgcggtcgt tcccgtggag acggtatccg ggaaggagga
     1021 gtggtcggcg ggcaccatcc ggaagaagat ccatcgccag ttaatgcgtt ttctcgagcc
     1081 agtcggacgc actcctgtcc aatactccac tccctctcag gaacatggag ggacccccta
     1141 ccatgaggca cacacactgc tcacccgtgt gaaagaaaag aatccgggac catcgccatt
     1201 gtacagcgga ggggagcacg aggcactcct gcgcctggag aagttcatca caggggacct
     1261 ttcccggtat gtctcggggc agaacgaccc tgcaagcccc gtcacctctg agttgagccc
     1321 gtacatccat ttcggacagg tctctcctgc gtatatcgcg cagagggtgc ttgaatcccg
     1381 ccatccgggt gccgatgcgt ttttggagca gttgatcatc cggcgcgagc tctcgttgaa
     1441 ctttgtgcac ttcaatcccc attacgacag gtacgagggg ttgcccgaat gggccaaaaa
     1501 gacgctcgcg gagcatgcag atgatccccg ggagtacgtc tacacggagg ccgagttcga
     1561 ggaagccagg acccatgacc ccttctggaa tgcagcgcag caggagctcc tggtcacggg
     1621 gaagatgcac gggtacatgc ggatgtactg gggaaagaag atccttgaat ggacggagaa
     1681 ccccgagcag gcatatgcca tagccctttc cctcaataac cggtatgaac tggatgggag
     1741 ggacccaaac ggctatgccg gtgttgcatg gtgcttcgga aagcacgacc gtgcctgggg
     1801 ggagcgccct gtccttggga aggtccgcta catgaatgcg gcgggcctca aaaggaagtt
     1861 ccggatagag cattacgtgg aacgggtcgc ggcgctcgcg gaggagcatg gcccgggcgc
     1921 ctcctgatga accgggagag gtgctctctc tgcgggaggt tgcggagttc caggagaagg
     1981 tataccggtt ttacagggtg cacaggaggg atcttccctg gaggaatacg cacgatgcat
     2041 attccatcct cgtatcagag atgatgctgc agcagaccca ggttgaacgt gtccttgagc
     2101 gttacccccg tttcattgcc cgtttccctg acttttcagc gcttgcgaag gcttcgtttc
     2161 ccgaggtgct cggggagtgg cagggactcg ggtataacag gagggccat
//
LOCUS       DAOQYR010000173         2195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_56808_length_2195_cov_9.946729, whole genome shotgun sequence.
ACCESSION   DAOQYR010000173 DAOQYR010000000
VERSION     DAOQYR010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56808_length_2195_cov_9.946729"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..955
                     /locus_tag="PKM87_12020"
     CDS             74..955
                     /locus_tag="PKM87_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNQ30839.1"
                     /translation="MTEIPKEEFILKCTSACAGCPSTLILRYVLKAAGPDTVLVVPAC
                     CTSVIQGIYPNTAMNVPVYNVAFAAAAACASGMSEAFQKSGKKTNVIVYAGDGGTVDI
                     GIQALSGAFERETDFLYICYDNEAYGNTGMQRSGSTPIGARTTTTPGGKVHAKKDLDA
                     IVAAHNPAYLATACAAYPQDLFKKVQKALTMRGSKFIHVLAPCPPGWRFSSEMTVEMG
                     KLAVRSGLWILYESEFGKVTINGPSKAAMIKPVPIEDYLSVQGRFKGIDRKAIDALKS
                     QAEQRAKHLQKEAEGIC"
     gene            949..2061
                     /gene="porA"
                     /locus_tag="PKM87_12025"
     CDS             949..2061
                     /gene="porA"
                     /locus_tag="PKM87_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HNQ30840.1"
                     /translation="MLTIATGNKAVAAAVKEVKPDVIAAYPITPQTEIVEQIAEYVTS
                     GELDSRYIPVESEHSAMAACIGASITGARTFTATSSHGLLYMHEMVNWAAGARLPIVM
                     ANVNRAIGPGWNVWAEHTDAFQERDTGWLQVYVSTVQEAYDTTLMAFRIAEHADVILP
                     VMVNLDGFLLSHSMQSLDTLKPGDFVPPLHLPHAIDVLNPAGYGTLTGPDEHFRFRWE
                     IEKSMRRSRKVIAETEGDFAERFGRSYGVSEEYRYEDAEVVVIAMGTLGKEAEVAVDL
                     LRSEGVKAGSLRIRWLRPFPDLDLSGRDVVVIDRDYSFGFGGVIAQSLIARSRVELYS
                     VIAGLGGQEVTYDDIAGFVRDRRPGTELWFGVNGNV"
     gene            2054..>2195
                     /locus_tag="PKM87_12030"
     CDS             2054..>2195
                     /locus_tag="PKM87_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNQ30841.1"
                     /translation="MYEIRIHSRGGQGGVTAARLLALAAFREGKYATACPFYGAERRG
                     APV"
BASE COUNT          483 a          599 c          656 g          457 t
ORIGIN      
        1 gcattcatcc ccaagaatta ccagaatact acgcttatta ctgatggaaa gctaaccttt
       61 ttcacgattt tccatgaccg agatccccaa agaagagttt atactgaagt gcacttcggc
      121 ttgtgcaggg tgtccctcta ccctcattct ccggtatgta ctcaaggcag caggacctga
      181 taccgtgctg gtggtccctg cctgctgcac cagtgttatc cagggcatct atcccaatac
      241 cgcaatgaat gttccggtat acaatgtcgc gtttgccgca gctgctgcct gtgcatccgg
      301 aatgagcgag gcgtttcaga aatccggtaa gaagaccaac gttatagtct atgccggcga
      361 tggtggaacg gttgacatcg ggatccaggc tctctccggt gccttcgagc gggagaccga
      421 tttcctgtat atctgctacg ataacgaagc ctacggcaac actggcatgc agcgttcggg
      481 ttccaccccg ataggtgccc gcaccaccac cacgccaggg ggaaaggttc atgcaaagaa
      541 ggatctcgat gccattgttg ccgcccacaa tcctgcctat ctcgcaacag cctgtgctgc
      601 ctacccccag gacctcttca aaaaggtgca aaaagcactc accatgaggg gctcaaagtt
      661 catccatgtc cttgccccct gcccacccgg atggcgcttc tcttcggaga tgaccgttga
      721 gatggggaaa ctcgcggtga gatcaggcct gtggatcctg tatgagagtg agtttgggaa
      781 agtgaccatc aatgggccct caaaggcggc gatgataaaa ccggttccca tcgaggatta
      841 tctttcagtc cagggccggt tcaaggggat cgacagaaag gctatcgatg ccttgaagtc
      901 tcaggcagag cagcgggcaa agcaccttca gaaggaggcc gaaggaatat gctgacgatt
      961 gcaaccggga acaaggcggt cgcggctgcg gtgaaagagg taaagcccga tgtcatcgcc
     1021 gcatacccga tcaccccgca gaccgagatt gtcgaacaga tcgctgagta tgtgacctcg
     1081 ggtgaactgg acagtcgcta catcccggta gagagtgaac attcagcaat ggcagcctgc
     1141 atcggcgcaa gcatcacggg tgcccggacc ttcactgcaa caagttcgca tggtctcctc
     1201 tacatgcacg agatggtcaa ctgggcagca ggggcacggc tccccatcgt gatggccaac
     1261 gtgaaccggg caatcgggcc gggctggaac gtctgggctg agcataccga tgccttccag
     1321 gaacgggata ccggctggct ccaggtctat gtcagcaccg tccaggaggc atacgacacc
     1381 accctcatgg cgttcaggat cgcggaacac gctgacgtga tccttcctgt gatggtcaac
     1441 cttgacggat tcctcttatc gcattccatg cagtcgcttg acacactgaa accgggggac
     1501 ttcgttccgc cgctccatct gccccatgcg atcgatgtac tgaaccctgc cggatacggg
     1561 actctcaccg gccctgatga gcacttcagg ttcaggtggg agatcgagaa gtcaatgagg
     1621 agatcccgga aggtcatcgc ggagacagag ggggactttg ccgaacggtt cggaaggagc
     1681 tatggggttt ccgaagagta ccggtatgaa gatgcagagg ttgtggtcat tgcgatgggg
     1741 accctcggta aggaggcgga agtggcggtc gatctcctca ggagtgaggg ggtgaaagca
     1801 ggatccctcc gtatcaggtg gctgcgcccc ttcccagatc tcgacctctc ggggcgggat
     1861 gtcgtggtca ttgaccgtga ctactcgttt ggattcggag gagttattgc ccagtccctg
     1921 attgccaggt cacgtgtcga gctgtatagc gtgattgcgg gtctcggagg ccaggaggtc
     1981 acctatgatg acattgcagg gtttgtgcgg gacaggagac ccggcaccga gctatggttc
     2041 ggggtgaacg gcaatgtatg agatacggat ccactcccgt ggcgggcagg gtggcgtgac
     2101 ggctgcccgg ctccttgccc ttgcggcctt ccgggaggga aaatatgcaa cggcgtgccc
     2161 attctacggg gcagaacggc gcggtgctcc ggtcg
//
LOCUS       DAOQYR010000174         2084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_61702_length_2084_cov_7.582060, whole genome shotgun sequence.
ACCESSION   DAOQYR010000174 DAOQYR010000000
VERSION     DAOQYR010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61702_length_2084_cov_7.582060"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            593..772
                     /locus_tag="PKM87_12035"
     CDS             593..772
                     /locus_tag="PKM87_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30842.1"
                     /translation="MRQFLSFFLVVAAVIMGISAAETTKEFLVWILPLLISWAQMPDT
                     SALINKVAVKGDGLS"
     gene            complement(858..1544)
                     /locus_tag="PKM87_12040"
     CDS             complement(858..1544)
                     /locus_tag="PKM87_12040"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HNQ30843.1"
                     /translation="MLPRVILHVATSLDGRITNFPANLDLYYALAATWKPDAILFGSE
                     TVLAAVRENPSLEVPQEHEDLFTPREGAEDTRPLLVIADSRGRVRCWDAIRTWPYMRD
                     LLAFCSAATPKEYLVYLAERKIDAIIAGGDRIDMRQALDQLNRRYGVKRVRVDSGGTL
                     NSVLLQAGMVDEVSVLVHPFLAGGKPDPTMFDPAKAGFPDLQVPLRLIRTEVVGDGLV
                     WARYSCENTA"
BASE COUNT          494 a          540 c          576 g          474 t
ORIGIN      
        1 tgaagacgtc caaaaatcct tttttcgctc ctgaaatacc ctgtctttct tccgggcgga
       61 gtggtggggg cccaggtgac gcagctaccc ggaaagggtt cagatttcat gctatgcaat
      121 aatctctcgg gaatttcgat gccgtggcgg tccatgatgc ccctgatgaa taagaggcga
      181 taaaagctgc catgaaacgg atcgaactca tggatgtgga gtcctctgtg gctatcccac
      241 gggaagaagt aaagagactg cttaccatga ctgagtattc cctttcgctc tccacaccat
      301 ggagatgaaa ccatcccctt ttccagtact tcctcacgat cttcttaccc tgtacagaaa
      361 aaccgtcact gcaaggaatg aatgtacacg tatcgctccc attgctctct tccctgaacg
      421 ttcaaaggaa tccggcacgg gttatcagta cgaacctccc gctccaagat atggttcttc
      481 gtgcgaacga ttaagggaag tggttcaaaa aaattatcat cgatcctgat tcctgtcctt
      541 cttacctctt acccaaaagg aggagcaata gcagataagg aggaacgata taatgagaca
      601 atttctttct ttttttctgg tagttgccgc tgttattatg ggaatatcgg ctgcggagac
      661 cacaaaagag ttccttgtat ggattttacc tctgttgata tcatgggcac agatgcctga
      721 taccagtgcc ctcatcaaca aggttgctgt gaaaggagac ggcctctcct gacggcgggg
      781 cctttccgga actcatcaga atcctttttt cgcacctgat cgggatcaca tttcatgagg
      841 tattctcata ggaacgctca tgcggtattc tcacaggaat atctcgccca gaccaggcca
      901 tctcccacga cttcggtccg gatgagccgc agggggacct ggagatccgg gaatcctgcc
      961 tttgccgggt cgaacatggt cgggtccggc ttccccccgg ccaggaaggg gtggacaaga
     1021 acgctgactt catcgaccat ccctgcttgg aggaggacgc tgttgagggt cccgccggaa
     1081 tcgaccctga cccttttcac accataccgc ctgttcagct ggtccaatgc ctgacgcata
     1141 tcgatccggt caccccctgc gatgatggca tcgatcttcc gttccgccag atagacgagg
     1201 tactccttcg gggttgcagc ggagcagaac gcaaggaggt cccgcatgta cggccaggtc
     1261 cggatggcgt cccagcaccg gactctcccc cggctgtcag cgatcaccag gagcggccgg
     1321 gtatcctctg ctccctcccg cggagtgaac agatcctcgt gttcctgtgg cacttcgagc
     1381 gacgggttct cgcggacagc ggcaaggacc gtctcgcttc cgaacaggat ggcatccggc
     1441 ttccaggtcg cggcaagggc ataatagaga tcgagatttg cagggaagtt cgtgatccgg
     1501 ccgtcaaggc tggtggcaac gtggaggatg acccggggta gcatgcgact ggctcctgat
     1561 cagaccttcc gcaggatgct atgaaaggtg atgcaataca gggtgaaggt ggaaacgagg
     1621 atgaacattc tttgatcgca caagtcgctg taatatgctg agagagaaag gagaatttat
     1681 ggttcaggaa aatatctcat gaaccatgga taaaaaagta gcattcgttc ttatgctgtg
     1741 tcttgttgga gcacttgttt tccctgcagc atccgcacca gtgatcgggc gatctggttc
     1801 aggatgcggg aataggggca ggggaacgca aaaaaaattc ccttctcgat cccatggtcc
     1861 ggtcaggagg gaaccacccc cccgaaacct tcaatggtga tggtgataat agggtggtgc
     1921 tgagggggag tgctcaatgg agagtgggga agagaaagcg atagcaatcg tctcgagagc
     1981 agcgaccgaa gcccacaagg gtcagaccag gaaggacggg aaaactcctt tcattgtcca
     2041 tcctgagcgg gtggcgaagt gcgtcgccca ttacgggggg aacc
//
LOCUS       DAOQYR010000175         2063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_62703_length_2063_cov_6.846614, whole genome shotgun sequence.
ACCESSION   DAOQYR010000175 DAOQYR010000000
VERSION     DAOQYR010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62703_length_2063_cov_6.846614"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PKM87_12045"
     CDS             complement(<1..292)
                     /locus_tag="PKM87_12045"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNQ30844.1"
                     /translation="MPGSSGHIAEYLIDHGHDVTVVDNLATGNMGNIQQLRDNPHFHF
                     VQGSITDSRLLNDVFRGSDGIFHQAAIPSVPRSIRDPVATNDANVNGTIAVLV"
     gene            740..1051
                     /locus_tag="PKM87_12050"
     CDS             740..1051
                     /locus_tag="PKM87_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNQ30845.1"
                     /translation="MKRARPPSTIKPILEDLRDMMPSLKERYQVASLSLFGSWVRGEQ
                     TDESDLDILVEFTEPPGLISFLILEEELSNRLGLRVDLVMKGSLKPVIGTRILREAVP
                     V"
     gene            1048..1179
                     /locus_tag="PKM87_12055"
     CDS             1048..1179
                     /locus_tag="PKM87_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30846.1"
                     /translation="MKAPRQSIDYLNDIIDAIEKAEAFSCGMTIDGFLADDKTLYAP"
     gene            1176..1397
                     /locus_tag="PKM87_12060"
     CDS             1176..1397
                     /locus_tag="PKM87_12060"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNQ30847.1"
                     /translation="MRAIEIIGEASKHVPESVRERYPDLPWKQMSGMRDKLIHAYFGV
                     DATVIWKTVSKDIPLIKNALQRVLKETSS"
     gene            complement(1625..1831)
                     /locus_tag="PKM87_12065"
     CDS             complement(1625..1831)
                     /locus_tag="PKM87_12065"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNQ30848.1"
                     /translation="MNYVITGDAGFIGAHLAGYLINRSHSATVVDNLFSGKKENLRYF
                     FDNPRFYFVQESITDPGLLKSRIP"
     gene            complement(1896..>2063)
                     /locus_tag="PKM87_12070"
     CDS             complement(1896..>2063)
                     /locus_tag="PKM87_12070"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNQ30849.1"
                     /translation="FSVSGHSGSHVRLSRIEGGTKVGTVVPLHDDVKPGTLHGILKLA
                     RIDQEEFYRYL"
BASE COUNT          541 a          510 c          532 g          480 t
ORIGIN      
        1 ccacgagcac cgcaatcgtc ccgttcacat tcgcgtcgtt ggtggcgacc gggtctcgta
       61 tcgatcgtgg cacggaaggg atcgcggcct ggtggaagat gccgtctgat ccacggaata
      121 cgtcatttag gagacgggaa tcggtgatgc tcccctgcac gaaatggaag tgagggttat
      181 cacgcaattg ctgaatattc cccatattcc ctgtagccag gttgtcgacc accgtcacgt
      241 cgtgtccgtg gtcgatcagg tattccgcga tgtgccccga tgaacccggc accgccggtg
      301 acgaggtagt tcatgttttt cccggattat cgtgttggaa tagcaattta cactggtatc
      361 cggggagtga taaaaatatc cgtgagtttt tagaggattt cctggtgaaa caaacatgag
      421 actgccgggt cacattcccc cccgatgaaa accgcggatg cggttttcat accaaaaatg
      481 ggcatacatg aagaatgagg cacaatgacc ccccctcttc atacccggat tcagtataat
      541 gacttgtgta cccgcctaca ggatgaaata gcgaggaata tacactggtg aattgcgtga
      601 tggtactctc atctgcgaga gagacgaggt agtatttccg acctttatcg gcagccgatg
      661 agagaagaga gaaaacctaa atagatacat cgtctttctg agtataacag aaagaaatca
      721 cgtaatcgag gtgattctgg tgaaacgtgc acgccctcct tccacaatca aaccaatcct
      781 cgaggatctt cgtgacatga tgccctcgct gaaggaacgt taccaggtcg cctccctttc
      841 tctctttgga tcatgggtcc ggggagaaca gaccgatgaa agcgacctgg acatactcgt
      901 ggagttcact gagcctccag gtctcatctc tttccttatc ctcgaagagg aactgagcaa
      961 tcggctggga ctgcgggtgg acctggtgat gaaaggatca ctcaaaccgg tgattggaac
     1021 ccgaatcctt cgcgaggctg taccggtatg aaagccccgc gtcaatcaat tgattatctg
     1081 aatgatatca tcgacgccat agagaaggca gaggcattct cctgtggtat gaccattgat
     1141 gggttccttg ccgatgacaa gacactctat gcaccgtgag agcaatcgag atcatcggag
     1201 aggcatcaaa acatgtccct gaaagtgtca gggaaaggta cccggacctc ccatggaagc
     1261 agatgtccgg gatgagggat aaattgatac atgcctattt tggagtagat gcaacggtta
     1321 tatggaaaac tgtcagtaaa gatattcctc tcatcaaaaa tgccctgcag cgggtactca
     1381 aagagacttc ttcctgacct cctgtacctg caattttgaa agcgaattct ttccactctc
     1441 tccagcaggg agagtggagc ctgatttccg acgacgacga tgcatatacc accttcctcg
     1501 ccccgcgctc cttcgccgcc atgagcacct ggagcatcct ttttccacgc atcgacgtcg
     1561 ttggtggcgg ccgggtccaa gatagaacgt gggacgaaag ggatcgccgc atggcacgac
     1621 cacatcacgg aatacgactt ttcaggagac cgggatcggt aatgctctcc tgcacaaagt
     1681 aaaaacgagg attgtcaaaa aaataccgga gattctcctt cttccccgaa aaaaggttgt
     1741 caaccaccgt tgcagaatgg ctgcgattga taaggtaccc ggcaaggtgc gccccgatga
     1801 agccggcatc gccggtgatg acataattca tgattctgcg aggttgtctc agtggagtag
     1861 aactacaaga cgagaggtag agttgacaaa aaatcttaaa ggtatctgta aaattcttct
     1921 tgatctattc gtgcaagctt gagaattcca tgaagagtgc ctggtttcac gtcatcgtgc
     1981 agagggacta cggtcccgac ctttgttcca ccttcaatcc tggagaggcg aacatgactt
     2041 ccggaatggc cggaaacgga aaa
//
LOCUS       DAOQYR010000176         2014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_65196_length_2014_cov_8.382338, whole genome shotgun sequence.
ACCESSION   DAOQYR010000176 DAOQYR010000000
VERSION     DAOQYR010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65196_length_2014_cov_8.382338"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..881)
                     /locus_tag="PKM87_12075"
     CDS             complement(48..881)
                     /locus_tag="PKM87_12075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ30850.1"
                     /translation="MDKNGYYQDKISIVTGANSGIGYALCEELLKRGGTVYLAGRDQK
                     KVAEAAGHLFAFRDHVRTLIVDVTKQEQVQKAIERTAKDAGRLDMLFNNAGIFKPMLF
                     ETGTLDDWKAMIDTNLWSVIYGVQAAVPIMLRQGSGHIVNTSSLAGLVPYPMQVIYDT
                     TKYAVVGLSESLRYEYAEKGIRVSVICPGDIATPVFSKIVDSKGQGAPIPPEAYPVDK
                     AASYILDHVAEGDGRIIVPYEPNHLIYGGYILGDKKVEEFLLKHAHDHRIRFEKEGRF
                     I"
     gene            1245..>2014
                     /locus_tag="PKM87_12080"
     CDS             1245..>2014
                     /locus_tag="PKM87_12080"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNQ30851.1"
                     /translation="MKQLSLIGGVRGTTIDAVYEKTKTPPKIHPDHPLPVVGFSCMTR
                     EPTDAEIPGLIDAAIAQVLGPQGLSAIIRPGDRVVIKVNIVGPSQGLPGEKGRGIISD
                     PRIVRYVAGKVREIIGFGGTADLKVIDAIFTPEKDPSDKNNPQSFYYARLQRSEGKSV
                     HESDVCYDYNADGILDGGSGAELVNLDAVPMSERFLTVVNEPIRGRSEVWFPKFLRTK
                     EQARGEADYCDVYIGIPVLKSHGFCGMTGALKLHWGTML"
BASE COUNT          440 a          577 c          539 g          458 t
ORIGIN      
        1 tcattgcatc tggttaccgt catcatttcc atcctccctc gcatcaatca gataaaacgc
       61 ccctcttttt cgaaccggat ccggtggtca tgggcgtgtt tcagcaggaa ctcctcaacc
      121 ttcttgtccc cgaggatata tcccccgtag atgaggtgat tgggttcata cggaacaata
      181 attcgcccgt cgccttccgc aacatggtca aggatgtacg atgccgcctt gtcaacggga
      241 tatgcttccg ggggaatggg tgctccctgc cccttgctgt cgacaatctt cgaaaagacc
      301 ggggttgcga tatccccggg acagatcacc gagacccgga tccctttctc tgcatactcg
      361 taccgcaggc tttctgacag cccgacgact gcatatttcg tcgtatcgta gatcacctgc
      421 atcgggtagg gcacgaggcc ggccagggat gaggtgttca cgatatggcc ggatccctgc
      481 ctgagcatga tcggaacggc agcctgcacc ccatagatga cgctccacag gttcgtatca
      541 atcatggctt tccagtcgtc aagcgtgccg gtttcaaaca gcatgggttt gaagatcccg
      601 gcattgttga acagcatatc cagccggcct gcgtctttcg cagtgcgttc aatcgccttc
      661 tgcacctgct cctgtttcgt aacgtccacg ataagcgtcc gcacatggtc cctgaacgcg
      721 aagaggtgtc cggcggcttc cgcaactttc ttctggtcgc ggcctgccag gtagaccgtc
      781 cctcctctct tcagcagttc ttcacagagg gcatatccga tccccgaatt cgcaccggtc
      841 acgatactga tcttgtcctg gtaatagcca tttttatcca tacctgtcct ccttcccttc
      901 tcctgacctt ttctccgata gccctgctcc ccatgcaatc gaggatggcc ttgacttcca
      961 tcaataccca tcacgccacc accctttcgg ccggatcccc gggactggaa tccatggcgc
     1021 ttcgccagtc caaacggtgg ggtatgagga atcgctacca cgtcaggttg atttccggaa
     1081 ggtattaatt atgatgatcc tattgctgat caaagagggt taccatgaat ccgtctgatg
     1141 atccagcatc gaacacctgc tcggatgcag gcaccacctc ccctccgggg ggtgttcatt
     1201 attccagcga tgtcagtcgc cgatgggaga aaattcacaa tgatatgaaa cagctctccc
     1261 tgatcggggg ggtccgggga acgaccatcg atgcagtcta tgaaaaaaca aaaacaccgc
     1321 cgaaaatcca ccccgatcat cccttaccgg tcgtagggtt ttcctgcatg acacgggagc
     1381 ccaccgatgc agagatccct ggcctgatcg atgctgccat cgctcaggtg ctgggaccgc
     1441 agggtctctc ggcaatcatc aggccggggg acagggtggt gatcaaggtc aatatcgtcg
     1501 gtccaagcca gggcctgcca ggggagaagg ggcgcgggat catctcagac ccgcgtattg
     1561 tgagatacgt cgccgggaaa gtccgggaga tcatcggatt tgggggaacc gcggacctga
     1621 aggtcatcga tgcgatattc actcctgaaa aggatccctc ggataagaat aatccacaaa
     1681 gtttttacta tgcccgctta cagcggagtg aggggaaatc cgtccatgag agcgatgtct
     1741 gctacgatta caatgccgat gggatactcg atggaggctc cggagcagag ctcgtcaacc
     1801 ttgatgcagt accgatgtca gagcggttcc tgaccgtcgt caacgaacca atcaggggga
     1861 ggagcgaggt gtggtttcca aagttcctcc ggacgaagga acaggcccgg ggagaggcag
     1921 actattgcga tgtgtatatc gggatcccgg ttctgaaaag ccatgggttc tgcggcatga
     1981 ccggggcgct caaactccac tggggaacga tgct
//
LOCUS       DAOQYR010000177         1940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methanolinea sp. isolate AMR_MDS_1005
            NODE_69388_length_1940_cov_7.385676, whole genome shotgun sequence.
ACCESSION   DAOQYR010000177 DAOQYR010000000
VERSION     DAOQYR010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852348
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanolinea sp. (wastewater metagenome)
  ORGANISM  Methanolinea sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanolinea.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:11:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,421
            CDSs (total)                      :: 2,378
            Genes (coding)                    :: 2,375
            CDSs (with protein)               :: 2,375
            Genes (RNA)                       :: 43
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 40
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 2 of 3
            Pseudo Genes (incomplete)         :: 1 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Methanolinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69388_length_1940_cov_7.385676"
                     /isolate="AMR_MDS_1005"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2268143"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="PKM87_12085"
     CDS             <1..174
                     /locus_tag="PKM87_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNQ30852.1"
                     /translation="YFLEFGASSLNFQLVVWTNDFSMTWETKDVINTRIAKRFAEEGI
                     EIPFPQMDVHMRT"
     gene            238..954
                     /locus_tag="PKM87_12090"
     CDS             238..954
                     /locus_tag="PKM87_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNQ30853.1"
                     /translation="MFGISSFCLHHYPLSHALDTLSSHTDLVEIMDDGPHFLESTELL
                     ESYSLRYAFHAPARSVNIACLHEPIRRASVEVIGQCFRIASEVGSNVVIHPGYFAWEE
                     ERERALRQYMKSLEDLKHLAGECGVTFYIENMANWNYFFLRTPEELDIIDGIGLALDV
                     GHAHLNHCLPGFLEATISHAHLHDNDGKDDSHSGVGQGTIDFVPVMEAIRRDHAVPVV
                     EVATLEGTLESIRVLEAMDR"
     gene            1088..1160
                     /locus_tag="PKM87_12095"
     tRNA            1088..1160
                     /locus_tag="PKM87_12095"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1121..1123,aa:Thr,seq:ggt)
     gene            1287..1511
                     /locus_tag="PKM87_12100"
     CDS             1287..1511
                     /locus_tag="PKM87_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ30854.1"
                     /translation="MQQSSRRSPPVRKIAGMKGFFREFIAERRGSAGISTGRANKIIF
                     TLICWRRFLPPFAELRMADIYTGIEAMRAG"
     gene            1525..1740
                     /locus_tag="PKM87_12105"
     CDS             1525..1740
                     /locus_tag="PKM87_12105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ30855.1"
                     /translation="MIAIMLRGGLITWKQPHKEVQTPPRPLFYATEEVADLFKVNVKT
                     IREIIKSKRLQAVRFGQEYRITDEQIR"
BASE COUNT          435 a          535 c          525 g          445 t
ORIGIN      
        1 tatttcctcg agttcggggc atcgagcctg aacttccagc tcgtggtatg gacaaacgac
       61 ttctccatga cctgggagac aaaggatgtg atcaatacga gaattgccaa gaggtttgcg
      121 gaagagggaa tagagatacc gttcccgcag atggacgtgc atatgcgcac ctagaacccc
      181 ccccattttc tctctcccaa acacaaccta tttcgcatcc cgttttgaga agaatgtatg
      241 tttgggatct cctccttctg cctccaccat taccccctgt cgcatgccct tgacacgctt
      301 tcttcccata ccgatctcgt cgagatcatg gatgacggcc cacatttcct cgaatccacc
      361 gaactcctgg agtcatactc gctccgctat gcattccacg ccccggcccg cagcgtgaat
      421 attgcatgcc tccatgaacc tatccggagg gcgagcgtgg aggttatcgg gcagtgtttt
      481 cgcatcgcca gcgaggtggg gagcaatgtg gtgattcacc ccggctactt tgcctgggaa
      541 gaggagcggg aacgggcgct tcgccagtac atgaaatccc tggaagacct gaaacatctt
      601 gccggcgaat gcggagtcac attctacatc gagaacatgg ccaactggaa ttatttcttc
      661 ctccggacgc cggaagaact ggacatcatc gatgggatag gccttgccct tgacgtcggc
      721 cacgcacacc tcaaccactg cctccccggg ttccttgagg caacgatctc ccatgcccac
      781 ctccatgaca atgacggaaa ggatgacagc cattccggag tcgggcaggg caccatcgat
      841 tttgtgcctg tcatggaggc tatcaggcgg gatcatgcag tccctgtggt ggaggtggcg
      901 acccttgaag gtacactcga gagcatccgg gtgctcgagg caatggacag gtgaggcagt
      961 caaggtccat gcgaggctgg ccgcgtgaat gcagccaggt aatagaaaag agcccgaata
     1021 tttcttttta tcactcctgt tccgcaatct tttaaagtgt atacgtcgac tgttgttgtc
     1081 gcattgcgcc tttgtggctc agtcggtaga gcgacacctt ggtaaggtgt aggtcggggg
     1141 ttcgattccc cccaaaggct ctgggcatca agttcgattc cccccgcgct atttataccc
     1201 ccaatgcgag atttctcttt atgatgcctt ccacttctgt gagtcagaga gagagtattt
     1261 tgggtgtatt caattcgaat atggcgatgc agcaatcatc caggcggtcg cctccggtca
     1321 gaaagatcgc cggcatgaag ggtttttttc gggaatttat tgccgagcgc agagggtcgg
     1381 cagggataag cactggccgg gccaataaaa tcatcttcac gctcatctgc tggcgtcgtt
     1441 ttctacctcc ttttgcggag ctcaggatgg cagacatcta caccgggatc gaggcgatgc
     1501 gggccgggtg atggcggtga gtcagtgatc gctattatgc tccgtggggg attgattaca
     1561 tggaaacaac cccacaagga ggtgcagaca cccccgcgcc cactttttta tgccactgaa
     1621 gaagtcgctg acctgttcaa ggtcaatgtg aagaccatac gcgagataat caagtccaag
     1681 cgtcttcagg cagtcaggtt tggtcaggaa taccggatta ccgatgagca gatccggtaa
     1741 tatatcgagg agaacaaaac aacgtgaccg gggatcccgg caccggccca ccatgttgca
     1801 aaggaagaca gtgccggccg gcccggtcga tcacagatag ggtggcctgg ttcatcacac
     1861 ttttgaacgg ctccggagga aaatcgttct tctcatgctg acccgccagc cgcctcccgc
     1921 ctgggcctcc acgactcgct
//
