LOCUS       DAOQZP010000001        34171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_1481_length_34171_cov_5.692314, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000001 DAOQZP010000000
VERSION     DAOQZP010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 34171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34171
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1481_length_34171_cov_5.692314"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1231)
                     /gene="rpoD"
                     /locus_tag="PKH60_00005"
     CDS             complement(140..1231)
                     /gene="rpoD"
                     /locus_tag="PKH60_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNQ16464.1"
                     /translation="MVTLPKALQADIEVLLKKARQNGFITQDDILDVFVEPELYIETL
                     DDLYDTAIQESIDIFESVAEPGDVEDTDALTRELEQLSALGSSGVSDPVRMYLKEIGR
                     IPLLNREEEIRLAQRVDNADPIAKQKLIDANLRLVVSIAKKYIGRGMTFLDLIQEGNK
                     GLIRAVEKFDWTKGFKFSTYATWWIRQAITRSIADQARTIRIPVHMVETINKLMRTSR
                     RLMQDLGREPTPEEIGKQMELEPERVREILKISQNTTSLEAPVGSGDDESVLGDFISD
                     DAQVSPYEATSLQMLRENVDEVLEALSDREAKVLKMRFGLSGNKMMTLEEVGRQFGVT
                     RERIRQIEAKALRKLKHPSRRKKLQDFLD"
     gene            complement(1331..2641)
                     /locus_tag="PKH60_00010"
     CDS             complement(1331..2641)
                     /locus_tag="PKH60_00010"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNQ16465.1"
                     /translation="MANNPHQQAVTQLENVAKLLEPQYTDGARFKKAIQKLKQPNYLH
                     ETALEVQMDDGTKRAFTAYRAQHDNARGPYKGGIRFHPGVTKEEVLALSTWMTWKCAI
                     AGIPYGGAKGGIVIDPKALSLAELERLSRAYAIWLTEFIGPWQDVPAPDVNTNGQIMT
                     WMVDAWAQSFAEKHPLSLNPQATFTGKPIEYGGSEGREEATGLGGVYVLEKLAEKLGW
                     QRKKDITIAIQGIGNVGYWFAHHADLLGYRVVAISDSRGGVYVESGLNPAKTLACKQE
                     TGSLDECRCTTDGCSIKNGRALTNDELLALPVDVLVPAALESVITQENAQTIQAKYII
                     EMANGPITPEADKILEKNKVLVIPDVLANAGGVTTSYFEWVQNLQGVHWKKELVLAQL
                     QPIMEQAFDEMWLKHTQMNVSARMAAYVTAVKLVVDTLLLRGVV"
     gene            complement(2631..3821)
                     /locus_tag="PKH60_00015"
     CDS             complement(2631..3821)
                     /locus_tag="PKH60_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015550868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNQ16466.1"
                     /translation="MQTIDESALALHKKYKGKLGTTALFPVKDKEMLSLVYTPGVGAV
                     SKAIAKDAQLSFEMTNRGRTIAVISDGSAVLGLGNIGPEAAMPVMEGKSLLMKLFADV
                     DSVPLVIKTQEVGEIINFVKLVAPTFAGINLEDISAPRCFEVEEALQDIGIPVFHDDQ
                     HGTAIVVMAALLNAAKVVKKPFEALNVVVVGAGAAGLAVTKMLLGIECQSASCRIIDG
                     AQRVKDVIVVDSQGALWAGRNNMNVYKQAVAGVSNKQKKQGALEEVIVSADVVIGVSG
                     PGAITASMVQAMAKDSIVFAMANPIPEIMPDVANQAGALVVATGRSDYPNQINNVLAF
                     PGIFKAVIDNCLPKITQAMKQSAALALAQLVTKPDRQNILPTAFDHSVVPAIALAVKE
                     AYGK"
     gene            complement(3826..4914)
                     /locus_tag="PKH60_00020"
     CDS             complement(3826..4914)
                     /locus_tag="PKH60_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ16467.1"
                     /translation="MSQDAQKLVIIGSGPAGYTAGVYAARAQLAPLLYAGYKAGGQLM
                     WTTDIENFPGFPNGKDGQTLMAEMRQQAERFGTVVLDKYVTAVDFSARPFKIWTNLPD
                     NFDPETHQFDSSTEFDKLMSKIKSESPATLAESVIVSTGASSIMLGIPGEKEFLGRGV
                     STCAVCDAAFYRDKNTVVVGGGDAAMEDALALTKFAKSVTVVHRRDSFRASKVMQERV
                     LQHEKVKVIWNSSLKKIAGEDKVEMAVVQTTDPQSNQVTTQEIVADGVFVAIGHRPVT
                     QLFQQALRLDPHGYIVTRQSVSQLGVSAANQALTPEGLVAFPTMTSVEGVFAAGDVVD
                     IRYKQAITAAGQGCQAALDAERWLEAKG"
     gene            complement(4911..5285)
                     /locus_tag="PKH60_00025"
     CDS             complement(4911..5285)
                     /locus_tag="PKH60_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNQ16468.1"
                     /translation="MMRREGTPIRQIETITNLDEILAAAGDSVVMVEFVADWCAPCKM
                     FRPILERAVNANAGVVKLYTVDSGLHSDIATKFGVSSIPHVLFFYQGEIIRRQVGFLP
                     EKQFSKLLEGLLVAIKPKEEAL"
     gene            complement(5288..5794)
                     /locus_tag="PKH60_00030"
     CDS             complement(5288..5794)
                     /locus_tag="PKH60_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNQ16469.1"
                     /translation="MTNKEIEAEIPGKEVGAAVESVQEQTGPNSMDEVGYEQPESLDF
                     DELLRDAGDTPVLIDFYADWCAPCKMLEPTIQQLANQYDGRVIVAKVNIEDNPDLITR
                     FNIFSIPRVAIFRNGEIAANFLGLKPFQEYVTTIDALLAEPEAESGVESEIDEMPSEL
                     ADVIAPAE"
     gene            complement(5812..6153)
                     /gene="trxA"
                     /locus_tag="PKH60_00035"
     CDS             complement(5812..6153)
                     /gene="trxA"
                     /locus_tag="PKH60_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNQ16470.1"
                     /translation="MSDTNKGAVHLDSASFDTTLAEAGDMPVLVDFYADWCGPCKMAA
                     PIIEEMAEEYAGKAIIAKVDVDANNELAGKFGVMSIPTVIVFKNGKEVKRQSGFAGKQ
                     GFIALLDAAMV"
     gene            complement(6183..6440)
                     /locus_tag="PKH60_00040"
     CDS             complement(6183..6440)
                     /locus_tag="PKH60_00040"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNQ16471.1"
                     /translation="MKKPDSIQSPDKVGAHLNRIKGQVEGIAKMYQEGRDCIEIVQQV
                     AAARNALGRVAKDLLSGEASRCSREKRFTDLDAVLKEVFRS"
     gene            complement(6474..6725)
                     /locus_tag="PKH60_00045"
     CDS             complement(6474..6725)
                     /locus_tag="PKH60_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HNQ16472.1"
                     /translation="MFGLNRKKDTNSTTVKFQISGMHCSSCSLNIDGELEDQPGVLSA
                     STSYAKSITTVKFDKKKIVPEKLMQTIRGLGYEVNQITA"
     gene            complement(6737..7792)
                     /locus_tag="PKH60_00050"
     CDS             complement(6737..7792)
                     /locus_tag="PKH60_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNQ16473.1"
                     /translation="MNLGLIFLTGLTTGGLSCVAVQGGLLTSMIANQKADGKHKASKV
                     TKIAGIELPRDDLLSLGMFLITKLIAHSILGFLLGLLGSVLTLNVTTRLMFQVFTALF
                     MLGTAMNLLQVHPIFRFLSFQPPRFVGRFIKDASKSKALFAPGILGLLTVFIPCGVTQ
                     AMEVLAINTGNPIQGAMIMFAFVLGTIPLFSIIGFILTKISTSWKNMFNKVAAYSLIA
                     LALYSLNGVAVVLDSPLSVQNLTGGITATIPGLGRGDTVFAEKNAGVQKIKIGIFNNG
                     YSPRDISVKQNIPVELTLESTDAYSCALSFELKEFGISTFLKSTDSKTFTFTPTKKGN
                     FTFACSMGMYSGTLRVL"
     gene            complement(7805..10087)
                     /locus_tag="PKH60_00055"
     CDS             complement(7805..10087)
                     /locus_tag="PKH60_00055"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNQ16474.1"
                     /translation="MPAKSTQKFNITGMHCASCANIIKRTLDKIEGVKASEVNYGTET
                     AQLDFDPQKTSVATINAKLDQYGYSLESGTTENASEHSQTDIVDQKIQALGQLKNKIK
                     IVIPFMMVSIFMMVWEIGGDQLQLFPPMTETVKEFFHHLLPLFATYTLFVIGAPYLQA
                     IRRFIQFRVANMDTLIGIGTFTAFMYSFVLSAFKTPLTAFFDVTQSYYDVTIVVIGFI
                     TIGKYLEARSKLKTGEAIKQLVGLQAKSAIIIRDGKEVEVAIDQVVVGDVIIVKPGQK
                     IAVDGVIVEGSSSIDESMITGESMPVDKGVGDTVIGATINKQGSLQIKATQVGSGTML
                     FQIVKMVEEAQGSKAPIEKLADQVSAIFVPVVLVLASAALLVWILVGQMFLPFSQAIS
                     LGVLSFVGTLVIACPCALGLATPTAVIVGVGKAAQNGILIKNAESLQKLHSVNYVVMD
                     KTGTLTQGKPTVTDIVPVGNISKKTVLQLLYSLEKHSEHPLAHAIMEKAESEKITAKK
                     VENFSAIEGRGLQGRIEKREYFAGNVGLAMELGIPISKAEVNKMTADGKTLVVLMTKT
                     ELLAYVAVADTIKPEALATVSQLHQLGVNVAMFTGDSLNTAKYIANQLGIDRVEAELL
                     PQDKLFKISQLQEEGYKVAMVGDGINDAPALAAADVGIAMGTGTDVAIESAGATLLGG
                     SIHKLPQTLSLSRATMRTIKQNLFWAFAYNVVLIPVAMGALYPINGWLLNPMLAGAAM
                     AFSSVSVVLNALRLRWVRLT"
     gene            complement(10077..10598)
                     /locus_tag="PKH60_00060"
     CDS             complement(10077..10598)
                     /locus_tag="PKH60_00060"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNQ16475.1"
                     /translation="MLTNAENIFIITNMYSELFRLQANLLKVLAQPKRLEIIHLLREQ
                     RLNVTEIYSMLDLPQANVSQHLMVMREAGIVTADREGKAIYYSLANPKIIEASDAIRE
                     WLLEQHQAKHDVDELVLSMKDLLPIVTDPVCGMRLSPKTAGFSTVYNGETIYFCASGC
                     HKLFTKKPRRYAR"
     gene            10631..11422
                     /locus_tag="PKH60_00065"
     CDS             10631..11422
                     /locus_tag="PKH60_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16476.1"
                     /translation="MLNRVENYPLIEHLEKEVEFFTAGVFVCIVNLDHLPQYLIDYLE
                     SPDSFGFDPDTLNHYLDQVSIWIIKEKTEKPNEFGLISTDLQTPPQETRKPNESLLTT
                     LTASLVEECLPLDQLNSTNAHTSFAYCRGISHLGHGTYQSPSEKHGIQDKYRADYFLV
                     GYSGESHSLQVRDEEVEPVGWITIAEYLSDSHPPRRLGGKAIVEESRKRLLEFLEMYW
                     KNPTVCQLPLTEVITNIEVRKESPDFNKRKFPPVVVAATESSPAS"
     gene            complement(11361..12614)
                     /locus_tag="PKH60_00070"
     CDS             complement(11361..12614)
                     /locus_tag="PKH60_00070"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ16477.1"
                     /translation="MPLGKSLSFHQHLRQKQTSFRFFIVTVLLFALGDGVFSYQVPIW
                     LGSVFHDVALVGIVMSSSSLVGFLCDLSFPTLFAKREYRFFFALTFIIALSTQLLGLL
                     SSSWLVAIFVMALWGVYYELFSFGLYHYLHTEIEPSQHASGWGITEALRSIALVTSPL
                     IVSLFLVLRLDPFILSSFLFGVSLVISSLFFFTNKKRVTKTTGEPEKAFSFVTFRVWS
                     TFLTKIWPVYLFYFCLIVFDATIWSVGIFFAEQLRQISPLGAFFIPAYIVPVLVAPLF
                     AGKLARRFGKKRSAFVIAIPLSLTLFIGHWALHSPLWHVGVMVLAGCFAGLILTESEA
                     IFEDYVTRLHRFSSEMIGLVRSASSLGYIIGPLVAGLLAEVLGIERTLAVIGLLPGMA
                     GIFAYFKMRGKTRLPQQQLEEISAY"
     gene            complement(12676..13425)
                     /locus_tag="PKH60_00075"
     CDS             complement(12676..13425)
                     /locus_tag="PKH60_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNQ16478.1"
                     /translation="MFELLTLISLVLFGSVLALIGGIVMLSVPRWSSFLSRYSVPFAG
                     GVLLTVSLAGVLPEAVEHLDHLAYYLVLATFLIIFLFEQFFFSLHHHDEEDGHHHVAK
                     RSAWFVMLGDTIHNAIDGVAIAASYLVNPGLGLVTAISTFLHEVPHEIGDFGILLKAK
                     WSKRSIISTNLFSALISVVAAVLVFWVEPPEEVLGILLSISAGLFLYLGASDFLPHAG
                     SGSISKRNMVVALLAGVGLMLFSMSIIPHAH"
     gene            complement(13431..15047)
                     /locus_tag="PKH60_00080"
     CDS             complement(13431..15047)
                     /locus_tag="PKH60_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16479.1"
                     /translation="MNKILIVFILVTGLVFWPILGKLDTTTTSPTDGPLIAWLVHHAA
                     DSIWGTSQLFQPPFFYPFKQTLTFSDPFVSSGFVAVVIQRLWPSITVLGQLNVQLMLG
                     TIGYLLALYWLVKVLGGRGAAAILLVTIGTFMPLRFIYVVHLHTYLLMGIPFGLACLI
                     LYTRGKQWKYLLGFFLGYLFQLLNAPMTAYFFVTVVGVYVFSQKQLWWELLTDRKLLL
                     IGLVLAIASVLFYLPYWQQAAYFHSARTIRDAAHFSFSINRLFSWDVLSLIFVNSVFF
                     ATSRKSKSLIKQSLPWLGVTLIGLCLMLGPVVKLDEQTVRVFGLPIPLPYFLVYYLVP
                     GMQAFRSVTRWSAVASLGLVLWSAVVFQTSRIKPAVKLGLLTLLAVLSVVSARRFLPV
                     FSVDAETPAMYQLAAKQPEKVMAMMPMFVWDMVPYEERESVRLLYQPGSGKIYYNGVS
                     GFMPPSRAQEIHLHFNSFPSDESITTLKNNGVQLLMVEYDQYQKMYDDGFVFGEQQVQ
                     DPLIIKSQLENRKDIQLIQCSNNDCLYRII"
     gene            complement(15040..17199)
                     /locus_tag="PKH60_00085"
     CDS             complement(15040..17199)
                     /locus_tag="PKH60_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ16480.1"
                     /translation="MENTTKPTSRPSLTFNQRVTKDVAVVKGLPKRIKGLGSWISHIA
                     LFNLILTVYLLHRFSTKYICKRASSHFMCRIVSENIDKAYNKVAALTTRNARTISRIN
                     LMDLAMRNMMFKKSRAFITVGGMAIGIGSIVFLVSIGFGLQQLVVSKVARLDELRQAD
                     VSLQPGQKEVINDETIAKVSQITGVDTTLPVIATVARVTYNNSISDVAVYGVTNDFLK
                     NSAIQPTHGKIYDSNEVAMAISGGKVAGTTRTIEEEFSMDDADEFAISFSIPEGVWLK
                     VRSGPEKTAPVLGYTKRGIAQQQGMLEWGDWYAEAAGDATRQREMEGKLQGAWIKADV
                     QLWEKAECDATSADCVDGNYIVLKDQFGSQQLAEDGYFAPIDIEFTNEKVLPVESRVL
                     GISQELTDAQVEALVLGTVDDTIDAATSAAGLSEVVSEDGEWVEIASESVVNEAEQVE
                     SITLANSAKKQAVVNTAMLELMGLKEDEAIGKSFSTSFVVVNDLLDSTDKRMESIPAD
                     YEIVGVIPEEKVPLFYVPFIDLRGLGISSYSQLKVIVDAQNNLANIRKQIESLGLSTR
                     SVADTVKQIDQLFGVVRVVLTALGLIALSIAALGMFNTLTVSLLERTREVGLMKTMGM
                     QSYEVQELFLTESMVMGFFGGILGILLGFGSAKLVGVALTAYSLSKGGGVIDIAYLPF
                     LYFVFVILLSLMVGIFTGIYPARRATKISALNALRYE"
     gene            complement(17183..17932)
                     /locus_tag="PKH60_00090"
     CDS             complement(17183..17932)
                     /locus_tag="PKH60_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ16481.1"
                     /translation="MPMPVIQIDKVTKSFEVGEQVIPVIKETSVEINQGEFFVLIGPS
                     GSGKSTLLHIMLGLEPPTTGEVRLLGQSIYKEQSHDQRAEFRKNHIGMVYQQSNWIKA
                     LNVVENVAFPLTMLGLPKTEAIEQSLKLLDKVGLSTWAYHMPTELSGGQQQRVALARA
                     LANNPEILIADEPTGNLDYQSGQAVMELFKTMNIEEKKTIIMVTHDIDYLRYATVAVQ
                     LFDGAVANIARGKEIMALTKNIKVKKSGKHN"
     gene            complement(17955..23966)
                     /locus_tag="PKH60_00095"
     CDS             complement(17955..23966)
                     /locus_tag="PKH60_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNQ16482.1"
                     /translation="MGKTLDHLSRVFVDICGGLKKLVFTNYFSYKKVQTKTRLLSISV
                     YGMMVGLSSVWFMVTVMPKIFASNDTYLWNFSTPGDYLVSDTNLVEVSAGSARLKIQE
                     YLSDAQTALLLHFNESSGDPVDSSSFTHSATSSSLLYQSGQLQNSAFFNGTTSAVFVN
                     DSAPLSFTGEHTLEAWTKFDDSFQAGVSNQRQGIIDKGDYQLYFDNETGKLVYELADS
                     QATTWTQHAGNYLNSSWDISGSPNIESIAVIGSNLYVGLGNALGDAQVWKWDGSVWTR
                     IGGDGINNSWGANTYETVQSMATDGTNLYVGLGLTAGDGEVWRWNGTTWFKVGGDAQY
                     GSWLVNTIEGVYSLYYQNSTLYAGLGLTADDAEVWSCTGCATDTPSWTKIGGDNGNLG
                     INGSWTGADNIEYVYSLTGDGTNIYAGLGLTAGDAEVWRWNGSSWTKVGGDGVGSAAS
                     WATSYEYVYSLFFQNNILYAGLGLTAGDAEVWSCTSCASGTPNWTKIGGDTVGWGTTY
                     EGVYSLTGDGTNIYAGLGLTGGEQEIWRWNGSGWTQVGGDGVYSGFTGANHGTVNQMV
                     FANNLLYAGIHSVNAAYSGQVWAFDPNAVPTPTWTWLGGDFKNESWSKRGINSIEVLQ
                     VSGDALYAGMGYQYAGNAYVWKMQEDNWSLVGGDGSNNGWTPYIYDKVSSMQSYEGDL
                     YVGLGVTANEADLYKLDGSTWSQVGGDGVGWGANFEEVLSQAVLGNYLFVGLGNSSDD
                     AEVWRYDGTNWLKVGGDDGGNGTVYNSWTGAMNIEQVLSLQPYKGKLYAGLGNTNNDA
                     AVWECTGCADPLATPSWTKVGGGGINGSWSTSSLDGFNNVDALLAYRDTLIATLGTGT
                     GEAEVWEYNGSNWIQIGGGANIRGSWDAGSYERVRTIGVYNGHLYAGLGNSTGDGEVW
                     RYNGLTWTNVAGDGVGWGVNVFEEINAISVYEGKLYVGSGNSASADATVWYIGNNRVL
                     KSSTTSQDTEWHHIAAKYDGTTMSLWRDGVSIGSLTTTVSMRDSSSPVIIGATHAGRE
                     FGKPRGGFEGMLDEVRISSVNRSELLTTPYSTNAQTVRPASPVFTSGIKEYDAFTSTF
                     NAGVGGSVTFRLSSDGGTTWKYLSGGNWTTSSSLVQSSLATDINTAMTTFPVTEQGLL
                     WQAIVDGDGVEQVSVSEVELGVITDTDAPTNPTTLTALQQAGGAALDTNPAIWHKQTA
                     PEFSWTGADDGAGSGVQGYYVYFGPSDTADPFHAGAFQTAATYTPSNMVSGTTYYLRI
                     KTKDAAQNIQATIWAPLTYKFDNTAPTNPASITVSPAGYAPTNEFTFSWPAGTDTGAD
                     ILGYQYKTGTASGTLSDWSATSSARLVTISDAAYKTDDNTFYLRTVDNAGNVSAVNLQ
                     ATYYFAGEGASAPQFLAVSEAKKTTNNFAFSWQAPVTHLGSASELTYCYSVNRKPTED
                     TCTFTSAGATSLAAGPFATQVGQNIFYLVAKNPDSAGGSINYGAYAEVVFEANTAAPG
                     IPLNVEIADVSVKATSSWKLALSWEKPEVSPETVSSYSIYRSTDGVTFSDTPLATTTG
                     IAYVDTGLLQQTYYYTVKACDNVENCGVATAVVSLLPDGRYTSAPALSSGPTVSNVTT
                     KAASISWSTDRSSDSKVQYGTSPGTYFVSEPSNSTKVTDHSIALSNLSPGTTYYYKAK
                     WTDEDGNTGVSAEKNFTTEPAPSVKDVSVPTLGLDSAIAQFTSTGASSVKIFYGTTTA
                     FGGVKEVSTSTSETTYTVTIDELLDGTKYYYKINAFDAEDNEYEGTILDFTTMPRPKI
                     STVRIQQVKNTAKSTVLVTWQTNTATSSIVTYYPTDAPTQAQDEVNVNLANGAHRILI
                     RGLLANTPYTLVVKGRDKAGNEAVSETQRFTTAIDTRPAQISDLRVESTIQSSGNDND
                     LTTAQLIVSWTTDEPATSQVEFGEGTGVTYTQQSQEDSSLTINHYVVISGLTPSKVYH
                     LRAISKDVVGNDTRSSDTVMITARATDDALGLVISNLGQVFSFLR"
     gene            complement(24057..25193)
                     /locus_tag="PKH60_00100"
     CDS             complement(24057..25193)
                     /locus_tag="PKH60_00100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNQ16483.1"
                     /translation="MQKRIQNSLLLGFIFVSTSYSAFSVTRILATSQLQDFRLYYLGA
                     QAVLLGKNPYEEVQGVIYPPISLVLLTPFASLPLDFAEDVWTLFSLIALLSSVLLLLG
                     VSKTFTMRNFFIVTGLAMLNFPVKFNLGMGQINLILLLLTCLSFFWYRKRLSVLAGSA
                     LAVAAALKLTPLVLVLFFLQKRQWKIVISCVVGFILLNGLGLTLLGTNATTDYWQKIF
                     PAIPTVGNAIYYNQAFTGWLARAEIADNVARVINYLIFGGMLAMSWLATRKKGQPVTV
                     ELSELGLFIISVLVGTGLAWQHHYVTLLIPFFAVLLLAMNLKISKVKYLVAIGIAYLL
                     VAANLKNPFAVLGIWQHIMLSHVLIGAVLLYIVTLVSLRGPTKQ"
     gene            25234..26133
                     /locus_tag="PKH60_00105"
     CDS             25234..26133
                     /locus_tag="PKH60_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16484.1"
                     /translation="MINAVTPAGEPITEPIPPTENIPSTTSPTATTLIDETTITPQTQ
                     PLVLNVMPPLSQTENQASNEPPPPTYLHPVQGSALPPNELTAIPAPFSGSTAPKYNGG
                     SDNQSVSKKKLFGYFLLGFLLNFLFLIPTVLWLALKSKVGTKQKFVAYLVGFVSSLVI
                     GAFFNGILTQFIGQKLTETIPSLAYVPQTISTVYPDLEASASVYYEKEFTNGAEISTS
                     TLTVNVTSQEPLDSNMYKQIGILACDALANRNESFDRVAVSQIGKKKVFILTIDTFAT
                     IDQTCFEWYGGPPPMLPDTSILE"
     gene            26163..26597
                     /locus_tag="PKH60_00110"
     CDS             26163..26597
                     /locus_tag="PKH60_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16485.1"
                     /translation="MTGVRLEQDGVIKPEDPSREIEAHLAYIDRLRGPITFDLVLEML
                     EKSVFKVDDFYQGLKPRIFFVYLKNNSDFQLEIKQLIDKKLSLVEIIEQAKQIILKYI
                     IQLIPHFFDRVFWEFVTQKYKEKLDNYARELPHYLLRVSQIT"
     gene            complement(26590..27150)
                     /gene="idi"
                     /locus_tag="PKH60_00115"
     CDS             complement(26590..27150)
                     /gene="idi"
                     /locus_tag="PKH60_00115"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF002995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HNQ16486.1"
                     /translation="MLDMNKSDQCKQMESGNETIMLVDSIDTVIGEIGKIEAHQKAGR
                     LHRAFTIFLQNQKDEWLITKRSEQKPLWPLWWDAACSSHPWFPNETIEQAALRRLPFE
                     LGLHIKEVNNVRVVGTYEYHAVYSPEWSENEINHIVVANYDGELLVNPNEVSDFKWLA
                     PSQIDERLTQPNLFSPWFAQAWQEVK"
     gene            27347..27802
                     /locus_tag="PKH60_00120"
     CDS             27347..27802
                     /locus_tag="PKH60_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16487.1"
                     /translation="MEALSATPTNETPEVLENQLGIDEAKLREAVLELSKDTDTLGEL
                     FEKFGLTEEEAVELQQQYGLSGTDWLDMLTFKSRDSAIQMVFMYVLMITAVIASFAMT
                     DAFIVEKGCTPPEFLASSLGVFAVITLMSFGSFLAFNILRDLKKRKKIK"
     gene            complement(27803..28216)
                     /locus_tag="PKH60_00125"
     CDS             complement(27803..28216)
                     /locus_tag="PKH60_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16488.1"
                     /translation="MQKTISIFVGLAVLMVVGIIIVSRVTNSQTPFSPDASQKILGEE
                     IQKDTDVAVPASLSMDCSQFFKQSGQEYQVSCDTFPKETVCSYYIVSREDQQSIQNLQ
                     YTNACAACRFYGEEGVKTIGTTQYEHLGYVTGVCE"
     gene            complement(28285..28950)
                     /locus_tag="PKH60_00130"
     CDS             complement(28285..28950)
                     /locus_tag="PKH60_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNQ16489.1"
                     /translation="MCGFFAFSTGMSAKQIADLFELTNMPKNEKDIQSIKFYPKSLIP
                     TVSKNSPNKLVMRYWSLIPRWWKEDPRKMKFSTFNARVEDIATKPTYRSAWSHHQRCL
                     IPATWFYEFQTLEVVGKPKAKKIPYKVEIEGEEVIGLAGLYETWTNGRQTVESCTIIT
                     CPAVASLKIIHNRQPVILTKDQWESWLNPAATYNDITALLHPTIQLSPSKISSDFFRT
                     ISE"
     gene            complement(28952..29680)
                     /locus_tag="PKH60_00135"
     CDS             complement(28952..29680)
                     /locus_tag="PKH60_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16490.1"
                     /translation="MKKIFLFLFGITALIVVGTVLVLGYLGFVPVISSLFGSDKPRDL
                     GVVATQENYDQYLAKADTTLKTLDAPVEGKSIVYSGSELLTTSFSQEEVTGRLNLSTW
                     KYMPIDQVQVKFSPDGTVEVAGRLRMDRLDGFIAQVGGTQYSKADVEKGLDYLGVLTV
                     NPVVYGKGTGSVTNNQVTAQIDYLEVGRLPVPLDKIDASGTFVSVANHIINNVPGLDV
                     QQADFTGGKLNFTGSVPTEMKVLQ"
     gene            29750..30631
                     /locus_tag="PKH60_00140"
     CDS             29750..30631
                     /locus_tag="PKH60_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNQ16491.1"
                     /translation="MNILITGHKGFVGRHFVKYFSTNSKNNIVGIDIVDNQDCRDFFK
                     TDVSSKGHWDLVIHLAAIVGGRQTIESDPIAVAIDLSIDAELFNWAVRQRPKRIVYFS
                     SSAAYPIALQTKTRRQKLSESDINLAKIAQPDLTYGWTKLTGELLAQFARDQYGLHVS
                     VLRPFSGYGEDQDLSYPFPSFIQRGKQKQNPFVIWGDGTQTRDFIHIEDIVAATLKTL
                     ELNLHQPINLGWGRPTSFNQLAKMVMKTAGYSAKLCHQLDKPIGVHYRVAHPKLLFSF
                     YQPKISLEEGIARALHV"
     gene            30624..31391
                     /locus_tag="PKH60_00145"
     CDS             30624..31391
                     /locus_tag="PKH60_00145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ16492.1"
                     /translation="MSNPHVSAICPIRDEQASILNIFETFKPLGGKTELIFVEGGSHD
                     YSWYLVQRLHKQTNQYGVHFRAYQQIGSGKASAVATGFDQARGNYLIIVDADMTVPQL
                     ELPKILSLFSQYGDRILASGNRLRGLPKPKSFYWINYLGNYFFRYYYSFILETQIQDI
                     SCGSKALTSRSWQKLKKTRLAEKSLDSWGDIDWLYYGRKIGLNPKFVQVDYVERVTGQ
                     SKLQNLVTRWQFALKMFTLGLYILKNRNWHETNQTNS"
     gene            31366..33069
                     /locus_tag="PKH60_00150"
     CDS             31366..33069
                     /locus_tag="PKH60_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16493.1"
                     /translation="MKLTKLIHRHEKKIETAFLAIFFTLLVIKLTIVFFQNLASPPLT
                     TDSWAYYSISKTIFEDFGFTNYIRQFEYISDYNISFPPLFPLAIRFSNSIIDLGIYAG
                     YLLNFGFAVAIGILLPKLTKLITGSALPGIFLLLLLLVNYHFLTELLGARSMILSLLL
                     LIGLIHIFLQKQLTQGHIAAIGALAGLLTLNRFDFLLPSILLGIVLLFVAQLTKIHTG
                     LIYSTVFLLVISPWIWYSYSHFGSWFVSDNSRSILLAAKTFTFDYYPNPEQIPTLFTQ
                     PFYWFAYRVIFKGVFSFIQFMKDVYYNPFFHQLFIGVGILLTTIAFVISQSGKIIRSK
                     LNFEPIRRLALLAPIFLLQLFSIGLTGFLDMRYTLFFLFYICLFLVGLVYLLLSTLLK
                     PMYVLIFCLVFLTILFFEQIIFSIPSDFLQLAQQPLNLNSQLLKPTGEYLQVNEAISQ
                     LDQTPILLVDTNQVATLSAAQFAGLTNIKTIETPSNLNDDVFLSLINNYKINFVYSNC
                     PKWLESLQNHFVITKTNLPKLLRVSVATDSATFTNLEYLTVRDEAYYQCNTSSSIDLI
                     K"
     gene            complement(33030..>34171)
                     /locus_tag="PKH60_00155"
     CDS             complement(33030..>34171)
                     /locus_tag="PKH60_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16494.1"
                     /translation="RGVTALWRLTIPYPQPVYPQGVTAMPGIGTANNVTGNTIRFPVT
                     DLTVSTTYGFFITNGGTGLISNPTSSTTIVHTIFTRTGSDAATADTKDVAVPIIANDQ
                     IVITATVAPTFTFVFNNNAQNLALAVGSVVSGTGTTITVTTNAASGWSAWALSANTAL
                     NSAASGDTIATTGTVNDSPDTLTPGVEGYVLDVDLTTDGAGGNHVYQIKADFVQVIHT
                     IMAQNPTIEFVLQPFLLFDQGFEYNGLRASTDIRLLFHHNQLFQCYLRMAKTDDFRCN
                     EHQGGQLVYIATSDVPEVIHAIAKKIVRKINKPQALYALDFAVSNAGRIYLVEGNIGP
                     GIDWDVTKEVNEKKSKQLIRRIVKEFAVRKNFSYLIKSIDDDVLH"
BASE COUNT         9926 a         8022 c         6522 g         9701 t
ORIGIN      
        1 gctatactat actgaagggt tttgaaatgc tactgaaaaa accagaaaaa ttcatcctca
       61 cgatgttaat tttatatatc ggctcgatgt cactgttgtt agtactagaa atatactttc
      121 gattaactca atagtgaaat taatccaaaa agtcttgcag ttttttgcga cgactgggat
      181 gctttagctt gcggagcgct ttggcttcta tttgacgaat acgttcgcgg gttacaccaa
      241 attgacgccc gacttcttcg agcgtcatca tcttgtttcc agataagcca aagcgcattt
      301 ttagcacttt tgcttctcga tctgacaacg cttcgagcac ttcgtccacg ttttcgcgta
      361 gcatttgtaa actggttgct tcgtagggtg aaacctgggc gtcatcagaa ataaagtcac
      421 ccaaaacact ttcgtcatca ccagaaccga ctggcgcttc caacgaagtt gtattctgtg
      481 agatcttcaa aatctctctc acccgctctg gctcaagctc catttgtttg ccaatttctt
      541 ctggtgttgg ctcacggccc aaatcttgca tcagccgtcg actagtgcgc atcagcttat
      601 taatcgtttc aaccatgtga actggaattc gaatagttct ggcctgatcg gcaatagaac
      661 gagtaattgc ttgtcgtatc caccaggtag cataggtaga aaatttaaaa ccctttgtcc
      721 aatcaaattt ttctacagct ctgatcaacc ccttattacc ctcttgaatt agatctaaga
      781 aagtcatgcc gcggccaatg tatttttttg caatagaaac taccaaacgg agattggcat
      841 caattagttt ttgtttggcg attgggtctg cgttgtcaac gcgttgagct agacgaattt
      901 cttcttcacg attcagcaac ggaattcggc caatttcctt cagatacatg cgcacggggt
      961 ctgaaactcc agacgaaccc agagcagaaa gctgttctag ttcgcgagtc agcgcatcgg
     1021 tatcctccac gtcgccaggc tccgctactg attcaaaaat atcgatactt tcctgaattg
     1081 cggtatcgta taaatcatca agagtctcaa tatacaactc tggttcaaca aaaacatcaa
     1141 gaatgtcatc ttgagtaata aaaccatttt gtctggcttt ttttaataac acctcgatat
     1201 cagcttggag tgctttgggt agagttacca tagttagttg aagatacgtt atacaacaag
     1261 taataaaaaa agcaaacaag accgcccact acaactgagt gggctgttct tatcccatta
     1321 atcttgcaca ttatacaaca ccacgaagca gaagggtatc gacgactagc ttcacagcag
     1381 ttacataggc tgccatccta gcagacacat tcatttgtgt gtgcttgagc cacatttcgt
     1441 caaatgcctg ctccatgata ggttgcaact gagctaagac caactctttt ttccaatgta
     1501 cgccttgtaa attctgtacc cattcaaaat atgaagtcgt tactcctcca gcattggcta
     1561 gtacatcagg aataacaagc accttatttt tttctagaat cttatctgct tcgggcgtaa
     1621 ttggtccatt ggccatttca ataatgtatt ttgcctgaat ggtttgggcg ttttcttgtg
     1681 taatcactga ctcaagcgcc gctggtacta gtacgtcaac cggcaatgca agtaattcat
     1741 cattagttag tgcgcgacca ttcttgatac tacaaccatc ggttgtacaa cgacattcat
     1801 caagagagcc tgtttcttgt ttacatgcga gagtttttgc gggatttagt ccagattcaa
     1861 catagacccc accgcgagaa tctgaaattg ccaccactcg atagcccagt aggtcggcgt
     1921 gatgggcaaa ccaataccca acgttgccaa tgccctgaat tgcaatagta atgtcttttt
     1981 tacgttgcca accgagtttt tctgccagtt tttcgagcac atagactcca cccaaaccag
     2041 tcgcttcttc tctaccttct gagccaccat actcgattgg tttgcctgtg aatgtagcct
     2101 gggggttcaa actaagtgga tgcttttcag caaaagactg agcccaagca tctaccatcc
     2161 aagtcatgat ttgaccattc gtattaacat ctggtgcagg cacatcttgc caaggaccga
     2221 tgaattcagt tagccaaata gcataggctc gactgaggcg ctcgagctca gcgagcgaga
     2281 gcgctttagg gtcaataacg attcctcctt ttgcgcctcc atacggaata cctgcaatgg
     2341 cgcacttcca tgtcatccaa gtggagagtg caagaacttc ctcctttgtt actccgggat
     2401 gaaaacggat tccaccttta tacggacccc tggcattatc gtgctgggct cgataggctg
     2461 tgaacgcacg ttttgtgccg tcatccattt gaacttctaa cgcagtttca tgcaaataat
     2521 ttggctgctt taatttttgt atggcttttt taaatctggc accatctgtg tactgtggtt
     2581 caagtaattt ggcaacattt tctagttgtg ttactgcttg ttgatgggga ttatttgcca
     2641 taagcttcct tcactgctaa tgctattgct ggtactacac tgtgatcaaa cgcagttggt
     2701 aaaatatttt gtcgatctgg ttttgttacc aactgagcaa gtgccaaggc cgccgattgt
     2761 ttcatcgctt gagtaatctt tggtaaacaa ttatcaatga ccgctttaaa tattccagga
     2821 aaagccagta cattattaat ttgattggga taatcacttc tccccgtcgc gaccaccaaa
     2881 gcacctgctt ggttggctac atctggcata atttcgggaa ttggattggc catagcgaag
     2941 acaattgagt ctttagccat agcttgcacc atagaggctg tgattgcacc tggtccagac
     3001 actccgatta caacatcagc gctaacaatc acttcttcca acgcaccctg cttcttttgt
     3061 ttgttagaga cacctgccac tgcttgttta tagacattca tattgttacg ccccgcccag
     3121 agtgcccctt gagaatcaac tacgatcaca tcttttactc gctgggctcc atcaataatt
     3181 cgacaactgg ctgattggca ctcaatgccg agcagcattt ttgtaacagc caaaccagct
     3241 gctcccgcac caactactac aacgttaagc gcctcaaatg gttttttaac tactttggcg
     3301 gcgtttaaca acgccgccat cacaactatt gctgtgccat gctgatcatc gtgaaagacc
     3361 ggaataccaa tatcttggag agcttcttct acctcaaagc accgaggcgc tgaaatatcc
     3421 tccaaattaa tgccggcaaa agttggcgcc acaagtttca cgaagttaat aatttcacca
     3481 acttcttggg ttttaattac caaagggaca gaatcaacat cggcaaacaa cttcatgagc
     3541 aacgacttac cttccatcac gggcatcgca gcttctggac caatattgcc tagaccaagc
     3601 accgcagaac catcggaaat aactgcaatt gttcttcccc tattagtcat ttcaaatgaa
     3661 agttgagcat ctttggctat tgctttagaa accgccccta ctccaggtgt gtaaaccaaa
     3721 gataacatct ccttatcttt gactggaaac aacgcagtgg tgcccagctt acctttatat
     3781 ttcttgtgga gtgctaaggc tgattcatcg attgtttgca tttcactagc cctttgcttc
     3841 tagccaacgc tctgcatcta aggctgcctg gcaaccttgg ccagccgcag taatggcttg
     3901 tttataacga atatccacca catcgcccgc ggcgaataca ccctccaccg aagtcatggt
     3961 tggaaatgcc accaatccct ctggagtgag tgcttgattg gccgcagaaa ccccaagttg
     4021 ggatacactt tggcgagtca caatatagcc atgcgggtca agccgtaatg cttgttggaa
     4081 caattgcgtg actggtcggt ggccaatggc cacaaataca ccatcggcaa caatttcttg
     4141 cgtagtgacc tggttgctct gaggatcggt agtttgtaca acagccattt caactttatc
     4201 ctctccagca attttcttca gacttgagtt ccaaattact tttaccttct catgttgcag
     4261 tacccgctcc tgcatcactt tactagctct aaaactgtca cgtcgatgaa caacagtaac
     4321 agatttggca aatttggtca gcgccaacgc gtcctccata gctgcatcac caccaccaac
     4381 cacaacagtg tttttatcac gataaaaagc ggcatcacat acggcgcagg tgctcactcc
     4441 cctgcctaag aattctttct cacctgggat acccaacatg atagaggacg cacccgtact
     4501 gacgataaca ctttcggcca gtgtcgcagg tgactcagac ttgatcttgc tcattagctt
     4561 gtcaaattct gtcgaagaat caaattgatg agtttctgga tcaaaattat caggcaaatt
     4621 ggtccaaatt ttaaacggac gagctgaaaa atctaccgca gtaacatact tatctagcac
     4681 gacagtacca aagcgctccg cctgctggcg catttctgcc atcaaagttt gtccatcttt
     4741 tccgttagga aagccaggaa agttctcaat atctgtggtc cacatgagtt gaccaccggc
     4801 tttgtagccg gcgtagagga gaggcgcgag ctgcgctcgc gcagcatata ctccagcagt
     4861 ataccccgcc ggaccagaac caataatcac cagcttttgt gcatcttgac tcatagcgcc
     4921 tcttcttttg gttttattgc taccaataat ccctcaagta acttactaaa ttgtttttct
     4981 ggtaaaaatc caacctgcct cctaataatc tcaccttggt aaaaaaacag gacatgaggt
     5041 atcgagctca cgccaaattt agtagcaata tcagaatgca atccagaatc caccgtatat
     5101 aactttacta caccagcatt tgcatttaca gctcgctcca gaatcggcct aaacatttta
     5161 caaggcgcac accaatcagc cacaaactca accataacta ctgaatctcc agctgctgct
     5221 aaaatctcat caagatttgt tatcgtttca atttgacgaa ttggagttcc ctctctgcgc
     5281 atcatattca ttcagctggt gctattacat ctgccaattc tgatggcatc tcatcaattt
     5341 ctgactctac gccactttct gcttcaggtt ccgctaatag ggcgtcaatt gttgttacat
     5401 attcttggaa cggtttcaac ccaaggaaat ttgccgcaat ttccccattt cgaaaaattg
     5461 cgactctggg aattgaaaat atattaaatc ttgtaataag atctggattg tcttcaatat
     5521 ttacctttgc cacaatgact ctcccatcgt attggttagc cagttgctgt attgtgggct
     5581 ccagcatttt gcatggtgca caccaatcag cataaaaatc aatcaataca ggagtatcac
     5641 ccgcatctct caataattca tcaaaatcta aagattctgg ctgttcatag ccaacttcat
     5701 ccatagaatt gggaccagtt tgctcttgga cgctctccac tgcagcccca acttcttttc
     5761 caggtatttc tgcttcaatt tctttgttag tcatattctc tcctttattt attaaaccat
     5821 tgcggcatcg agcaaagcta taaagccttg tttaccagcg aatccagatt gacgcttgac
     5881 ctctttgccg tttttgaaca cgataactgt tggaatgctc atcactccaa atttaccagc
     5941 aagttcgtta ttggcgtcta catccacctt agctataatc gctttgccag catactcctc
     6001 agccatttcc tcaataattg gcgccgccat tttacatggt ccacaccaat ctgcataaaa
     6061 atctacgagt actggcatat ctccagcttc tgcaagcgta gtatcaaaac tagctgagtc
     6121 tagatgaact gctcctttgt ttgtgtctga cataaaactc cttataatac tttttattac
     6181 tactacgatc taaagacttc ttttaagact gcgtccaaat cggtgaagcg tttttcacga
     6241 gaacatcggg aagcttcacc cgaaagcaaa tctttggcta cgcgaccaag cgcattacga
     6301 gcggcggcca cttgttggac aatctcaatg caatctctac cctcttggta catcttggca
     6361 ataccttcca cctgaccttt gattcggttg agatgagcgc ctactttatc gggactttga
     6421 attgaatcag gttttttcac gatcctcaat ataccccctg ggggtattaa ttgtcaagca
     6481 gtaatttggt ttacttcgta ccctaagcca cgaatggtct gcatcagttt ctcgggaact
     6541 atcttttttt tgtcaaattt cacagtagta atacttttag cataactggt agaggcactc
     6601 aaaacacctg gttgatcctc caattctcca tcaatattta acgagcaact agagcaatgc
     6661 ataccactaa tttgaaattt tactgtggta ctattagtat cttttttgcg atttaaacca
     6721 aacatatttt gtctttttat agtaccctca gcgtgccgct atacatcccc atcgagcagg
     6781 caaaggtaaa gttgcccttc ttcgtcgggg taaaggtaaa tgttttacta tctgtagatt
     6841 ttagaaaggt ggaaatacca aattctttga gctcaaacga aagggcacat gaataggcat
     6901 ctgtactctc tagcgttaat tcaactggaa tattttgttt tactgatata tctctgggag
     6961 aataaccatt attaaaaatt ccgattttaa ttttttgcac acctgcattt ttttcggcaa
     7021 agactgtgtc tccccgaccg agtcctggaa tcgtcgctgt aattccccct gtcagattct
     7081 gaactgatag tggcgaatcc agtaccactg ctacaccatt aagactatac aaagctagcg
     7141 caattaacga atacgcagca actttgttga acatattttt ccagctagtg gatattttgg
     7201 ttaaaataaa tccaatgata ctaaaaagtg gaatcgtacc taagacgaat gcgaacataa
     7261 tcatcgcacc ttggattgga ttacccgtat taatcgccag cacctccatt gcttgagtga
     7321 caccacaggg aataaacacc gtgagtaaac ccaaaattcc tggcgcaaat aaagccttgc
     7381 tcttactagc atccttgata aatctgccaa cgaaacgcgg tggttgaaaa cttaaaaatc
     7441 ggaagatagg atgcacttgc agtaaattca ttgccgttcc caacataaac aaggcagtaa
     7501 atacttgaaa cataagcctg gtcgtgacat taagtgtgag caccgatcct agcaaaccaa
     7561 gcaaaaaacc caaaatactg tgcgcaatta attttgtaat caaaaacatg cctaaagaaa
     7621 gtaaatcgtc gcgtggcaac tcaatcccag caattttggt aaccttactc gccttgtgct
     7681 tgccatccgc cttctgattg gcaatcatgc tggtgagtaa acctccttgg actgccacac
     7741 atgaaagtcc tcctgtggta aggccggtta aaaagattaa tcctaaattc attgctcctt
     7801 tatattatgt gagacggacc caacgcaagc gcaaagcatt cagtacgacg ctgacgctag
     7861 agaacgccat cgctgctccg gccagcatag ggttcaataa ccaaccattg ataggataca
     7921 gcgcccccat agcgacagga attaacacca cattgtaggc gaaagcccaa aagaggttct
     7981 gtttgattgt gcgcatcgtg gctcgggaaa gtgatagggt ttgtggcaac ttatggatgg
     8041 aaccaccaag taaagtagcg cctgcagact caatcgctac gtctgtgccg gtccccatag
     8101 ctattcccac atctgcagct gccagtgcgg gcgcatcatt gataccatcg cccaccatcg
     8161 caactttgta cccttcctct tgtaattggc tgattttgaa taatttatcc tgaggcaaca
     8221 gctcagcttc aactcgatca atgccgagtt ggttggcaat atactttgcg gtgttcaagg
     8281 aatcaccggt aaacattgcc acattgactc ccaattgatg taactgagaa actgttgcta
     8341 atgcctcggg ttttatcgtg tctgcgacgg caacgtatgc aagtagctct gtcttcgtca
     8401 ttaatactac gagcgttttg ccatctgccg tcattttgtt tacttccgct ttagaaatag
     8461 gtatcccaag ctccatagcc aagccaacat tgccagcaaa atactcccgt ttttcaatcc
     8521 taccctgcaa gcccctgcct tcaattgcag aaaaattttc tacttttttg gcagtgattt
     8581 tttctgactc agccttttcc ataattgcat gtgcaagcgg atgctctgag tgtttttcaa
     8641 gtgagtacaa gagttgcaag actgtttttt tagaaatatt tcccaccggc acaatgtctg
     8701 tgactgtggg cttgccttgg gtcaacgtgc cagttttgtc cattactaca tagttgacgc
     8761 tgtgtagttt ctggagtgac tcagcatttt taatcaaaat gccattttgt gctgctttac
     8821 ctacgcccac aatcactgct gttggcgtcg ccaagcctaa ggcacaggga caggcgatga
     8881 ctaaggtgcc aacaaaactt aaaacgccga gactaatagc ttgcgaaaaa ggtaaaaaca
     8941 tttgaccgac taaaatccaa acgagaagag ctgcggaagc cagaaccaaa actactggca
     9001 caaaaattgc cgatacttgg tcagctaatt tttcaattgg agctttggaa ccctgggctt
     9061 cttctaccat tttcacaatt tgaaataaca tggtgccgct accaacctgt gtagctttga
     9121 tttgtaatga accctgctta ttgatagtgg ctccaatcac tgtatcgcca acacctttat
     9181 cgaccggcat tgattcgccg gtgatcatcg attcatcaat tgaacttgag ccctcaacaa
     9241 tgactccatc aacagcgatt ttttggccag gttttacaat aattacgtct ccaactacga
     9301 cctgatcaat tgccacctcg acttcttttc catcacgaat aattattgca ctttttgctt
     9361 gtaatccgac taattgcttg attgcttcgc ctgtttttaa cttcgaacga gcttctaagt
     9421 atttcccgat ggtgataaag ccaattacca caattgtcac gtcgtaatag ctttgcgtca
     9481 catcaaaaaa cgctgtaagt ggagttttga atgccgagag aacaaaacta tacatgaaag
     9541 ccgtgaacgt accaatgcca attaaggtat ccatatttgc cacgcgaaat tgaatgaagc
     9601 gtcgaatagc ctgcaaataa ggagcgccga tcacaaatag tgtatatgta gcaaacagcg
     9661 gtaaaaggtg gtgaaaaaat tctttgacag tttccgtcat cggtggaaac agttgtagtt
     9721 gatctccgcc aatctcccag accatcataa aaatacttac catcataaag ggaatgacta
     9781 ttttaatttt gttttttagc tgacccaatg cttgaatttt ttgatcaaca atatccgttt
     9841 gtgaatgctc agaagcattt tccgtagttc cactttccaa tgaataacca tattggtcta
     9901 attttgcatt aattgtagct actgaagttt tttgtggatc aaagtctagt tgagcggttt
     9961 cggtaccata atttacttcg ctggccttca cgccctcaat tttatcgagt gtgcgtttaa
    10021 taatattggc gcacgaagca cagtgcatac cagttatatt gaatttttga gtggatttag
    10081 cgggcatatc tccttggttt cttagtaaat aatttatggc aaccactggc gcaaaaataa
    10141 atagtttctc cgttgtacac agtagaaaat cctgcggttt taggagaaag acgcatgcca
    10201 caaactgggt cggtcacaat tggcaagaga tctttcatgc tcagcactag ctcatctaca
    10261 tcatgcttgg cttgatgttg ttcgagcaac cattcgcgaa ttgcatcgct ggcctcgata
    10321 atttttggat tggccagcga ataataaatt gcttttccct cgcgatcggc ggtcacaatg
    10381 cccgcctcgc gcatcaccat taagtgttga ctcacattag cttgcggtaa atctaacatt
    10441 gagtagattt ccgtgacatt caagcgctgt tctctgagta aatggataat ttccaacctt
    10501 tttggctggg ctagtacctt gagtaaattg gcttgtaatc gaaacagctc gctatacata
    10561 ttcgtaatta tgaatatgtt ttctgcgttt gtcaacatcc actccaaatg actgtataat
    10621 atgcgttctg atgctaaata gagtagaaaa ttatccctta attgaacact tagaaaaaga
    10681 agtggagttt ttcacagcag gcgtttttgt gtgcatcgtc aacttagatc atttaccaca
    10741 atatttaatt gattatttag agtcgccaga ttcattcggc tttgatcctg acaccctcaa
    10801 ccattacctt gatcaagtca gcatttggat aattaaagaa aaaacagaaa agccaaatga
    10861 gtttgggctg atatcaactg acctacagac acctccccaa gaaacacgca aaccaaatga
    10921 atcgttacta accacgctta ccgcttctct agtggaagag tgtctgccgc tagatcaact
    10981 gaactcaacc aatgcacata catcatttgc ctattgtcgg gggatatcac accttggtca
    11041 tggcacctac caatctccaa gtgaaaaaca cggaattcaa gataaatatc gagctgatta
    11101 tttcttggtc ggctactccg gggaatctca ctctcttcaa gtgcgcgatg aagaagtcga
    11161 accagtcggc tggattacca ttgctgagta tttgagtgat tcgcaccccc ctcgcagact
    11221 cggaggaaaa gcgatagttg aagaatcgag aaaaagactt ctagaatttt tagaaatgta
    11281 ttggaaaaat cccactgttt gccaactacc actaaccgag gttatcacta acatagaagt
    11341 aagaaaagaa tctccagatt tcaataagcg gaaatttcct ccagttgttg ttgcggcaac
    11401 cgagtcttcc cccgcatctt gaagtaggca aatatgccgg ccattccggg taataaacca
    11461 atcactgcta acgtgcgctc aattcccaaa acttctgcca ataatccagc cactaaggga
    11521 ccaataatat agcccaagct tgaagctgag cgcaccaaac caatcatttc actgctaaac
    11581 cgatgaagcc tcgtcacata atcttcaaaa atcgcctctg attctgtcaa aattaaccca
    11641 gcaaaacaac cagccaaaac catgactcct acatgccaaa gcggagaatg taacgcccag
    11701 tgaccaataa acaacgtcaa tgaaagcgga atagcaatca caaaagctga gcgttttttg
    11761 ccaaagcgtc tggctaattt tcccgcaaat agcggtgcta ctaagactgg tactatatat
    11821 gcgggtataa aaaatgcacc caaaggcgag atttgacgca gttgctcggc aaaaaatatt
    11881 cccacgctcc aaatggtggc atcaaatacg attaggcaga aataaaaaag atacactggc
    11941 cagattttcg taaggaaagt tgaccaaaca cgaaaagtga caaatgaaaa tgctttttct
    12001 ggttcgccag ttgttttggt gacacgcttt ttgtttgtga aaaagaacag cgaactaatc
    12061 acgagcgata caccaaacaa gaagctggaa agaataaacg gatctaggcg caagactaaa
    12121 aacagactta caatcagtgg agaggtaacg agagcaattg atctcaaagc ctcagtaatt
    12181 ccccatcccg atgcgtgctg agatggctca atttcagtgt gcaagtaatg atacaagcca
    12241 aatgaaaaaa gttcataata cacgccccac aaagccataa caaaaatagc caccaaccag
    12301 cttgatgaca gcaaaccaag taattgagtg gataaagcaa taataaatgt gagcgcaaaa
    12361 aagaatcgat actctctttt ggcaaataag gtgggaaagc tcagatcgca caaaaatcca
    12421 accagcgaag aactgctcat gacaatgcct accagggcca cgtcgtgaaa caccgacccc
    12481 aaccaaatcg gcacttggta cgaaaacact ccatcaccca gcgcgaataa taagactgtg
    12541 acaataaaga acctgaaact cgtttgcttc tgacgcaaat gttggtggaa gctcaatgat
    12601 tttcccaatg gcatagctca gtaacaatct ctatatcgtg actagtatag aggaaaaatt
    12661 gattgaaacc taggattaat gagcgtgcgg gatgattgac atgctgaaca gcatcaagcc
    12721 aactcccgcc agtaaggcca ccaccatgtt gcgcttggag atcgaaccac tccccgcatg
    12781 tggcaaaaag tcgctggctc caagatacaa aaataatccc gcagaaattg aaagtaatat
    12841 ccccagcact tcttccgggg gctccaccca aaaaaccagt acagctgcca caacggaaat
    12901 tagcgctgaa aacaagttcg ttgaaataat gctgcgtttg gaccatttgg ctttgagcaa
    12961 gatcccaaaa tcaccaattt cgtgaggcac ttcgtgtaaa aatgtagaaa tcgctgtaac
    13021 tagacccaaa cctggattga ccaaatagct cgctgcaatt gccacaccat caattgcatt
    13081 gtgaattgta tctcccaaca tcacaaacca agcagagcgc ttagccacat ggtgatgccc
    13141 atcttcttca tcgtgatgat gtaaactaaa aaagaattgt tcaaacagaa agataattaa
    13201 aaatgttgct aacacaagat aatatgcaag atgatcaaga tgctcaactg cttctgggag
    13261 aactccggca agtgagactg tcagcaacac tcctccagca aatggcactg agtagcgaga
    13321 taaaaatgat gaccagcgag gtacggacag catcactatt ccgcctatga gggcaaggac
    13381 gctaccaaag agtacaagtg aaataagggt caatagttca aacataagtg tcagattata
    13441 cgatacaagc aatcattatt actacattga attaattgaa tatctttgcg attttctaat
    13501 tgagatttaa ttatgagtgg atcttgaact tgctgttcgc caaagacaaa gccatcatca
    13561 tacatttttt gatattgatc gtactcaacc attagtaatt gaacaccatt atttttgagt
    13621 gtagtaattg attcgtcgct gggaaatgag ttgaagtgca gatgaatttc ttgcgcgcga
    13681 gacggtggca tgaatcctga tacgccgttg taataaattt tgcccgaacc tggctggtac
    13741 aacaagcgca ccgactcgcg ctcttcataa ggcaccatat cccatacaaa catcggcatc
    13801 attgccataa ctttttctgg ctgttttgca gccaactggt acatcgctgg agtttcagca
    13861 tcaacagaaa aaactggcaa aaatcttctt gctgaaacaa cactaagcac agcgagtagc
    13921 gttaacagtc ctagtttaac agcaggtttg attcggctgg tttgaaaaac cactgcagac
    13981 caaagtacca ggcccaaact ggcaactgcg ctccaacgcg tcacagatcg gaatgcctgc
    14041 atgcctggga ccaaataata gactagaaaa tacggcagag gaatcggcaa gccaaaaaca
    14101 cgcactgttt gttcatcaag tttcaccacg ggacccaaca tcaaacataa accaataagt
    14161 gtaacaccta accaaggcaa gctttgcttg attaacgact tgctttttcg actggttgca
    14221 aaaaatacac tgttaacaaa aatcaaacta agtacgtccc aagaaaataa ccgattaatc
    14281 gaaaatgaaa aatgagcggc atcccgaatg gtgcgggcag aatggaagta cgccgcttgt
    14341 tgccaatatg gtaagtaaaa taacacactt gcaattgcga gcaccaagcc gatcaacaac
    14401 aattttctat cggtaagtaa ttcccaccaa agttgtttct gactaaatac atacactcca
    14461 accacggtca cgaaaaaata ggctgtcatg ggtgcattca acagctgaaa caagtatcct
    14521 aaaaaaaaac ctagtaaata tttccactgt ttaccgcgag tatacaaaat tagacaagct
    14581 aaaccaaagg ggatacccat tagtagatag gtgtgcagat gaacgacata gataaatcgc
    14641 agaggcataa aggtaccaat agttactaac agaatggcgg ccgctccgcg gccgcccaac
    14701 acttttacta gccaatacaa agccagcaaa tacccgatgg taccaagcat caactgcaca
    14761 ttgagttgtc ccaatactgt gatgctcggc caaagccgct gaatcaccac agcaacaaag
    14821 ccagaactca caaacggatc agaaaaagtg agtgtttgtt tgaacggata aaaaaagggt
    14881 ggttgaaaca gctggctagt accccagatt gaatctgcgg catgatgaac caaccatgca
    14941 attaatggac catcggtagg tgatgttgta gtggtatcta acttaccaag aattggccaa
    15001 aaaaccaaac cagtaaccaa aataaaaaca attagaattt tattcatacc gcagcgcatt
    15061 caaggcgcta atctttgtcg cgcgccgagc aggataaatt ccagtaaaaa tgcccaccat
    15121 caacgaaagc aaaataacaa aaacaaaata gaggaacggc aaataggcta tgtctatgac
    15181 acctccgccc tttgaaagtg aataggcagt taaagccaca cctaccaact tagccgaacc
    15241 aaacccaagc aagattccca agataccacc aaagaatccc attaccattg attctgtcaa
    15301 aaagagttcc tgcacttcat atgactgcat tcccatagtt ttcatcaagc caacttcccg
    15361 agttctttcc aacagtgaaa ctgtcagggt gttgaacata ccaagcgctg caatcgacaa
    15421 ggcaatcagc cccaaagcgg tcagcaccac tctcactaca ccgaaaagtt gatcaatctg
    15481 tttcactgtg tcggcaaccg acctggtaga caagccaagt gactcaatct gttttctaat
    15541 gttagccaaa ttgttttgtg catcaactat cactttaagt tgagaataag aactaatgcc
    15601 taaaccacgc agatcaataa atggcacata aaacagtggt actttctctt ctggaattac
    15661 acccacaatt tcgtagtcag caggaattga ttccattcgt ttatcggtgg aatccaataa
    15721 gtcattaacc actacaaacg aggtactaaa tgatttgcca attgcctcat cttccttgag
    15781 tcccattagc tctagcatgg cagtatttac aacagcttgt tttttggcac tatttgctaa
    15841 agtaatagat tctacctgtt cagcctcatt caccacagac tcagaagcaa tctctaccca
    15901 ctcaccgtcc tcactgacaa cttctgaaag gccggcggct gatgtggcag catcgatagt
    15961 atcatccacg gttcccaaaa cgagcgcctc aacctgagca tcagtgagtt cttgtgatat
    16021 cccaagcacc cttgattcaa ctggtaaaac tttttcattc gtaaactcaa tgtctatggg
    16081 ggcaaaatat ccatcttcgg ctaattgctg agaaccaaac tgatccttta acactatgta
    16141 attcccatca acacaatctg cgctagttgc atcacactca gctttttccc aaagctgtac
    16201 atcggctttg atccatgctc cttgcaactt tccttccatc tcgcgctgac gagtcgcatc
    16261 tccagccgct tcagcatacc aatcacccca ttccaacatg ccttgttgtt gggcaatacc
    16321 tcgtttggtg taacctaaaa ctggcgcggt tttttctggt ccagatctga ctttaagcca
    16381 aacaccttca ggaatgctaa aactaatcgc aaactcatca gcgtcatcca tactaaattc
    16441 ttcttcaata gttctagtag ttcctgccac tttcccccca gatatcgcca ttgccacctc
    16501 gtttgaatca taaattttgc catgagtggg ctgaatagca gagttcttca gaaaatcatt
    16561 ggtaacgcca tagaccgcca catcactaat cgaattattg taggtaaccc tcgccacagt
    16621 cgcaattact ggcaacgtgg tatcgactcc agtaatctga ctcaccttag cgatagtttc
    16681 gtcatttatg acttcttttt gaccaggctg taaggaaaca tccgcttgtc gtaattcatc
    16741 aagtctcgcc acttttgaaa ccaccaattg ttgtaagcca aagccaatcg aaactaaaaa
    16801 cacaattgaa ccaataccaa ttgccatacc tcccacagtg ataaacgccc tcgatttttt
    16861 gaacatcatg tttcgcatgg ccaaatccat gaggttgatc cgactgatgg ttctggcatt
    16921 tctggtagtg agagctgcga ccttgttata cgccttatcg atattttcgc taacgattcg
    16981 gcacatgaag tgagagctgg ctcgtttaca aatatatttg gttgaaaacc gatgtaataa
    17041 atagaccgtt aggattagat tgaacaacgc aatgtgagaa atccaactac ccaatccttt
    17101 aattcttttt ggtaatccct ttactacagc tacatccttg gtcacccgct ggttgaaggt
    17161 taaacttggt ctgctggtag gtttagttgt gttttccact ctttttcacc ttaatatttt
    17221 tggttaacgc cataatttct ttgcccctgg ctatattggc tactgcgcca tcaaacagtt
    17281 gcacggccac agtggcatag cgcaaataat caatgtcgtg agtaaccata ataatcgttt
    17341 tcttttcttc aatattcatg gttttgaata actccatgac cgcctggccc gactggtaat
    17401 ctaaattacc cgtcggttca tcagcaatta atatttctgg attgttagcc aacgcccgag
    17461 ccaaggccac ccgctgttgc tgcccaccag acagctcagt tggcatgtgg taggcccaag
    17521 tggacaaacc aactttatct agtaatttaa gcgattgctc gatagcttct gtttttggca
    17581 aacccaacat tgtcaaggga aaggctacat tttcaactac attgagtgct tttatccaat
    17641 ttgattgctg atacaccatc cctatgtgat tcttgcgaaa ctccgcgcgt tgatcatgac
    17701 tttgctcttt gtagatactc tgacccagca gtcgaacttc tcccgtggtg ggtggttcaa
    17761 gccccaacat gatatgcaac aaggttgatt tgcccgaacc tgacggccca atcaaaacaa
    17821 aaaattctcc ttggttaatc tcaacactgg tttccttaat tacgggaatt acttgttcgc
    17881 caacttcgaa acttttagtc actttgtcta tttgtattac tggcattggc atatttgtgt
    17941 gtttcttgat tttcctatcg taaaaagcta aagacttgtc ctaaattaga aattacgagt
    18001 ccaagcgcat catcagtggc gcgcgcggtg atcatcacgg tgtcggatga acgcgtgtca
    18061 ttacccacga catctttaga aatcgcgcgc agatgataga ctttggaagg agttaatcca
    18121 gaaataacca cgtagtgatt gatggtcaga ctgctatctt cttgactttg ttgagtgtag
    18181 gttacgccgg ttccttcacc aaactctacc tgactggtgg ccggttcgtc agtagtccac
    18241 gacacaatca gttgggcagt ggtgagatcg ttatcattac cgctcgattg tatggtagat
    18301 tcaacgcgca aatcggagat ttgcgcgggc cgcgtatcga tggctgtggt aaatcgctga
    18361 gtttctgaca ccgcttcgtt acccgcctta tctcgccctt tgactaccaa cgtgtagggt
    18421 gtgttggcca agagacctcg aatgaggatg cgatgagctc cgttggcgag attaacgttg
    18481 acttcatcct gagcctgcgt tggggcatct gttggatagt aggtgactat gcttgaagta
    18541 gcagtgttgg tttgccaagt cactaaaact gtggatttgg cagtattttt aacttgctga
    18601 attctcacgg tactaatttt tggtcgaggc attgttgtaa agtctagaat cgtgccctca
    18661 tattcattgt cctcggcatc aaaggcattg atcttgtagt aatatttagt gccatcaagt
    18721 aattcatcaa tagtgactgt gtacgtagtt tcacttgttg acgtagagac ttctttgact
    18781 ccgccaaagg cggtagtcgt gccatagaat attttgactg agcttgcacc agttgaagta
    18841 aactgcgcaa ttgcagaatc caacccaagc gttggcacac ttacatcctt gacggagggt
    18901 gcaggttccg ttgtaaagtt tttttctgca gaaacaccgg tattaccgtc ttcatcggtc
    18961 catttggctt tgtaatagta agttgtccca ggtgataagt tgcttaaagc tatggaatgg
    19021 tcagtgactt ttgttgagtt tgaaggttct gatacaaaat aggttcccgg actcgtccca
    19081 tattgaactt tagaatctga gctgcggtcg gtagaccaac taattgaagc agcctttgta
    19141 gtaacgttac ttacagtagg cccagaactg agagctggtg cagaagtgta acgtccatct
    19201 ggtagcagcg aaaccactgc agtagctacg ccacaattct ctacgttgtc acaagctttg
    19261 actgtatagt aataggtttg ttgtaacaac ccagtatcca cataggcgat acctgtggtg
    19321 gtggctaatg gtgtatccga aaatgtcact ccatctgtgg atcgataaat tgagtagctg
    19381 ctcacggttt ccggactgac ttctggtttt tcccaagaca atgctagttt ccatgaagac
    19441 gtggccttta ctgaaacatc agcaatttct acattgagag gaattcctgg agcagcagta
    19501 ttagcttcaa aaacaacttc agcatacgct ccgtagttaa tgcttcctcc agcggaatca
    19561 ggatttttgg caactaggta aaaaatattt tgccccactt gggtagcaaa aggaccagca
    19621 gcaagcgaag tggcgccagc gctggtaaaa gtacaggtat cttccgtcgg ttttcggtta
    19681 acactataac aataggtaag ttcgctggcg cttcccaaat gtgttactgg agcttgccaa
    19741 gaaaaagcaa aattattggt ggtttttttt gcttccgata ctgccaaaaa ctgcggtgca
    19801 cttgcacctt ctcccgcaaa ataataggtt gcctgcaaat tcaccgctga aacatttcca
    19861 gcgttgtcaa ccgttcgtaa ataaaaggta ttatcgtctg ttttgtaggc agcatctgaa
    19921 atggttacta aacgagcact ggaggtagct gaccagtcac tgagtgttcc agaagcagtg
    19981 ccagttttgt attgatatcc caagatatct gctcctgtat cggtacccgc tggccaggaa
    20041 aatgtaaatt catttgttgg tgcatagcca gcaggtgaaa ccgtaatgct agctgggttg
    20101 gtgggcgccg tattatcaaa tttataagtg agcggcgccc aaatggtcgc ttgaatattt
    20161 tgcgccgcat ctttagtttt tattctcaga taataggtag taccactcac catgttactt
    20221 ggagtgtacg tcgcagctgt ttgaaaagca cccgcatgaa agggatccgc agtgtcggat
    20281 ggtccaaaat acacataata cccttgcaca ccgctgcctg cgccatcatc agcgcctgtc
    20341 caactaaatt ccggcgctgt ttgcttgtgc caaatagctg gatttgtatc tagggcagct
    20401 ccaccagctt gctgcaatgc agtcagggtg gttgggttgg tgggcgcatc ggtatcggta
    20461 attactccca gttccacctc acttacagaa acttgttcga ctccatcacc atccacaatt
    20521 gcttgccaaa gcaaaccttg ttccgttacg gggaatgtgg tcattgcagt attaatatcg
    20581 gtagccagac ttgattgtac taagctcgat gaagtcgtcc aattacctcc agaaagatac
    20641 ttccaagtcg tgccaccatc actagataat cgaaaggtga cagagccacc gacgccggca
    20701 ttaaaagtac tggtaaatgc atcatactcc ttaatgccag atgtaaatac tggtgaagcg
    20761 ggacgaactg tttgagcatt agtagaatac ggagtagtga gtaattcact ccgattaact
    20821 gaactgatcc gaacttcgtc cagcattccc tcaaaaccac cgcgtggttt accaaactct
    20881 cttccagcat gtgttgcacc aataatcact ggtgacgacg agtctctcat agatactgtg
    20941 gttgtgagtg aaccaatgga aaccccatct cgccacagac tcatggttgt gccatcgtac
    21001 ttggcagcaa tatgatgcca ctcagtgtct tgtgaggtgg tagaagactt aagtactcga
    21061 ttgttgccga tgtaccacac cgtagcgtct gcactggcag aattgccaga ccccacatat
    21121 agtttacctt cgtacactga aatagcattt atttcttcaa acacatttac gccccaaccc
    21181 actccatcac cagcaacatt cgtccaggtt aaaccattgt agcgccaaac ttcaccatct
    21241 ccagtactat tacctaagcc agcgtataag tgaccgttat acacaccgat agtgcgcact
    21301 cgttcatacg aaccagcatc ccaagatcct cgaatgttag cccccccgcc aatttgaatc
    21361 caattactac cgttgtattc ccatacttct gcttctccag tacccgttcc gagcgtggca
    21421 atcaacgtat ctctatatgc tagcagcgca tctacattgt taaaaccatc taacgagcta
    21481 gtagaccaac taccattgat tcctccaccc cctactttgg tccagctggg cgtggctagt
    21541 ggatctgcac agccagtaca ctcccatacc gccgcatcat tattcgtgtt gcccaagcca
    21601 gcatacaatt tacctttata cggttgtagc gatagtactt gctcgatatt catcgcgccg
    21661 gtccagctgt tgtataccgt cccattacca ccatcatcac cgccaacttt gagccaattg
    21721 gtaccatcgt accgccaaac ttcagcatca tccgacgagt tacccaatcc aacaaacaag
    21781 taattgccca aaacagcttg cgaaagtacc tcttcaaagt tagcgcccca acccaccccg
    21841 tccccaccca cctgactcca cgtggatcca tcaagtttgt ataagtctgc ttcattcgca
    21901 gttactccaa gaccaacata taaatcacct tcatatgatt gcatggatga aactttgtca
    21961 taaatatagg gagtccaacc gttgttggat ccatctccac cgactaaact ccaattgtcc
    22021 tcctgcatct tccacacgta ggcattgcca gcatattgat atcccattcc tgcatacaat
    22081 gcatcacccg atacctgcaa cacttcgatt gaattaatac ctcgcttgct ccaactttca
    22141 tttttaaaat cacctcccaa ccaagtccaa gtgggtgttg gcactgcatt tggatcaaaa
    22201 gcccacacct gccctgaata ggcagcattc accgagtgaa tacccgcata tagtaagtta
    22261 ttagcaaaca ccatttggtt gactgtgcca tgatttgctc cagtgaaacc actgtaaact
    22321 ccgtctccac caacttgtgt ccaaccactg ccgttccaac gccagatttc ctgttcacca
    22381 ccagttaaac ctaatccggc gtagatattg gtaccatcac ccgtaagtga ataaacacct
    22441 tcataggtag tgccccaccc aacagtgtcg ccgccaattt ttgtccaatt tggtgtacca
    22501 ctcgcacaac tggtgcagct ccaaacctca gcatctcccg cagttaaacc caaaccggca
    22561 tataaaatat tattttggaa aaaaagacta tacacatatt cgtaactagt ggcccaactg
    22621 gcagccgaac ccactccatc tcctccaacc ttagtccagc tactgccatt ccaacgccag
    22681 acttcagcat ctcctgcagt taatcccaat ccggcataga tattggtacc atcgccagtt
    22741 agagaataga catattcaat attgtccgct cccgtccaac taccattaat gccgaggttg
    22801 ccgttgtctc cacctatttt ggtccagctg ggagtatcag tagcacaccc cgtacagctc
    22861 caaacctcgg catcatccgc agtcaatccc aaaccagcgt agagggtgct attttgataa
    22921 tacaagctat acacaccttc tatagtgtta accaaccaag aaccatactg agcatctcca
    22981 cccaccttaa accacgttgt gccattccag cgccaaacct ctccgtctcc tgccgttaat
    23041 cccaacccca cgtacaaatt tgtcccgtcg gtagccattg attgaaccgt ttcatatgta
    23101 ttggctcccc aagaattatt gataccatca ccaccaattc tagtccaaac actcccatcc
    23161 catttccaaa cttgagcatc acccaaggca ttacctaaac caacatacag attactgcct
    23221 ataacagcaa tgctttctat gttaggcgat ccagaaatat cccaactgct atttaagtaa
    23281 ttacccgcgt gttgagtcca ggtggtagct tggctatctg ccagctcgta gaccaacttg
    23341 ccggtttcat tatcaaaata gagttgataa tcacccttat caatgattcc ctgccgttga
    23401 ttacttactc cagcttgaaa actgtcgtca aactttgtcc aggcttccaa tgtgtgctct
    23461 ccggtgaatg aaagcggcgc tgaatcattc acgaatactg cgctggtagt accgttaaaa
    23521 aacgcactgt tttgcagttg gcctgattgg tacagtaatg agctagaagt tgctgagtga
    23581 gtaaaggagg aagaatccac cggatctcca cttgattcat tgaagtgcag taacaaagct
    23641 gtctgggcat cagaaagata ttcctgtatt tttaaccgag cagaaccagc agagacttca
    23701 actaaattag tatctgatac aaggtaatca cccggtgtag aaaaattcca taggtaggta
    23761 tcgttggatg cgaaaatctt gggcatcaca gtgaccataa accacaccga gcttagtcca
    23821 accatcattc catatacaga tatgcttaag agtcgagttt tagtttgtac ttttttgtat
    23881 gagaagtaat tagtaaatac tagtttcttc aaaccaccac aaatatcaac aaaaactcga
    23941 cttaaatgat cgagggtttt acccatacga gagttactat atagaaaaat tgcggttggg
    24001 ggaatacaga gaaagtactg ttaaatcggc aattattttt ttaaagattt tgtactctat
    24061 tgtttcgttg gacctcgtag actcaccaat gtaactatgt agagtaaaac cgccccaatt
    24121 aatacatggg aaagcataat gtgttgccaa atacctaaaa cagcgaatgg attctttagg
    24181 ttggcagcta ctaacaaata tgcgattcca atggcaacaa gatatttcac ttttgagatc
    24241 ttcaagttca ttgcgagcaa taaaaccgca aaaaatggaa ttagcaaagt cacataatga
    24301 tgttgccaag ctaaacccgt acccactaaa acagaaatga tgaataatcc tagctcactc
    24361 aattcgacag ttactggttg tccttttttc cgtgtagcaa gccaactcat ggcgagcata
    24421 ccgccaaaga tcaaataatt gatgactcta gctacgttgt cggcaatttc tgctcgagct
    24481 aaccaacccg tgaatgcttg attgtaataa attgcattac caacagtggg aatagcggga
    24541 aatatctttt gccaataatc agtggtagcg tttgtaccga gtagcgtcag tcccaaacca
    24601 ttcaacaata taaatcccac cacacaactt attacaattt tccactggcg cttttgcaaa
    24661 aaaaacaaga ctaggactaa tggtgtaagt ttgagcgccg cagcgaccgc caaggcgcta
    24721 cctgcaagta cagacaatcg ctttcgatac cagaagaatg ataagcacgt taacagtaac
    24781 aaaataagat tgatttgccc catacccaag ttaaatttga ccggaaagtt tagcattgcc
    24841 aagccagtaa caatgaaaaa attgcgcatc gtaaaggttt tagaaactcc caacaataaa
    24901 agaactgaag agagcaatgc aatcaaagaa aatagagtcc aaacatcttc tgcaaaatct
    24961 agaggtagac tcgcaaatgg agtcagcaat accaatgaaa ttggagggta aattacaccc
    25021 tgaacttctt catacggatt tttacccagt aaaactgcct gtgctcccaa atagtacaaa
    25081 cgaaaatctt gtaactgtga tgtcgccaga attctagtaa ctgagaatgc tgaataggag
    25141 gttgaaacaa aaataaaacc taagagtaaa ctattctgta ttctcttttg catttggtag
    25201 cattctatcg ctttttacgc tacgattata accatgataa atgcagttac tcccgctgga
    25261 gaaccaatta cagaacctat cccaccaaca gaaaatatcc cgtcaacgac cagcccaacg
    25321 gcgactactt taattgacga gaccaccatc acacctcaaa ctcagccgct tgtactgaat
    25381 gttatgccac ctcttagcca aacagagaat caggcttcaa acgaaccgcc gccgccaacg
    25441 tatttacatc ccgttcaagg ttcagcgctt cctcctaatg aactcactgc aattccagct
    25501 cctttttctg gcagcactgc accaaagtac aacgggggct cagacaacca atcggtctca
    25561 aaaaagaaac tttttgggta ttttttactc ggctttttgc ttaattttct ttttctcatc
    25621 cccaccgtgc tttggcttgc cttaaagagc aaagttggta caaaacaaaa atttgtggct
    25681 tatctggtgg gttttgtgag ctctctcgtt attggcgcat ttttcaacgg tatccttaca
    25741 caattcattg gccaaaaatt aactgaaact attcccagtt tagcctacgt accacaaacc
    25801 atcagtactg tgtatccaga tctagaagca agcgcttcgg tctactatga aaaagaattc
    25861 accaacggag cggaaatctc aacatccaca ctcacagtta acgtgacatc gcaagaacca
    25921 ctcgattcaa atatgtataa acaaattggg attttagctt gtgatgcact tgctaatcgc
    25981 aacgaatcct ttgacagagt tgcagtgagt caaattggca aaaagaaagt gtttattctt
    26041 acaattgata cttttgccac tatcgatcaa acctgttttg aatggtacgg aggacctcct
    26101 ccaatgttgc cagatacatc aattctggaa tagtcgacag agtttgttat aattcaatat
    26161 atatgacagg tgttcgttta gaacaagatg gagttatcaa accagaagat ccctcaagag
    26221 aaatagaggc tcacctggct tatatcgatc gcttgagagg accgattact tttgatttag
    26281 ttttggaaat gctggaaaaa tcagtattca aagttgacga tttttaccaa ggtttaaaac
    26341 cacgaatttt tttcgtgtat cttaaaaata actctgattt tcaacttgag attaaacagt
    26401 tgatcgataa aaaactatct ttagtagaaa ttattgaaca ggcaaaacaa ataatactca
    26461 agtacattat ccagttgata ccccacttct tcgacagggt tttctgggaa tttgtgaccc
    26521 agaagtataa ggaaaaactg gataactatg cccgagaact tccacactat ttgttacgag
    26581 tatctcaaat tacttgactt cttgccatgc ttgcgcaaac caaggtgaaa atagattagg
    26641 ctgcgttaat cgctcatcaa tttgtgatgg agcaagccac ttaaaatctg aaacctcatt
    26701 tgggttaacc aacaactctc catcatagtt tgctacaaca atgtggttga tttcattttc
    26761 tgaccattcg ggagaataga ctgcgtgata ttcgtaggta ccgacaactc ttacattatt
    26821 tacttcctta atatgcaaac caagctcaaa aggcaaacgt cgaagagcgg cttgctcaat
    26881 cgtttcattg ggaaaccaag gatgagatga gcatgccgca tcccaccaca acggccacaa
    26941 tggtttttgt tcacttcgtt tagtaattaa ccactcgtct ttctgatttt gcaaaaaaat
    27001 ggtaaaggct ctgtgaagtc tacctgcttt ttgatgagcc tcaatttttc caatctcgcc
    27061 aatcaccgtg tcgatactat caaccaacat aatggtttcg tttccggatt ccatttgttt
    27121 acattgatca cttttattca tatctaacat ggaaatagta tatcagcgtt gtcatcacta
    27181 catgcttgct tacattaaaa atacatcacg catatacagc tttggtttat agtaaagaac
    27241 ggctccagag agtgttatgc tttagtgtaa actaattgtt aaagcacttg tttcctcttg
    27301 acgaaaaatg ctttactagt acaatctata tcagaaagag aggtatatgg aagctttatc
    27361 tgctacacct acgaacgaaa cccccgaagt cttagaaaat cagcttggca tagatgaagc
    27421 aaagctgcgt gaggcagttc ttgaactgag caaagatact gataccttgg gcgaactatt
    27481 tgaaaaattt ggattaactg aagaagaagc tgttgagtta caacaacaat acggactttc
    27541 tggtacagat tggctagata tgcttacttt caagtcaaga gattcggcta tacaaatggt
    27601 tttcatgtat gtgctaatga taacggcggt tattgcatca tttgcgatga ccgatgcgtt
    27661 tatcgttgaa aaaggctgta ctcctccaga attccttgct agctcactag gtgtttttgc
    27721 agtaataacc cttatgtcat ttgggtcatt tctggctttt aatatacttc gagatctaaa
    27781 aaaacggaaa aaaattaagt aattactcgc aaacgcccgt tacgtatccc aaatgttcat
    27841 attgggtcgt cccgatggtt tttacccctt cttctccata gaatctacac gcagcgcaag
    27901 catttgtata ctgcaaattc tgaatcgatt gttggtcctc tctcgaaaca atgtaatagg
    27961 agcagacggt ttctttggga aaggtatcgc aagacacttg gtattcctgg ccagattgtt
    28021 taaaaaactg gctacaatcc atcgataagc tggcaggtac tgccacatcg gtatcttttt
    28081 gaatttcttc accaagaatt ttctgtgaag catcggggga aaacggcgtt tgagaattag
    28141 tcacacggct cacgatgata attccaacga ccattaagac agctaaacca acaaagatgg
    28201 aaatagtctt ctgcataaaa gtatcctccc aatcttggaa tatacaccta cataataaat
    28261 atgtctatct gaatcccttt gaaactattc tgatatggtg cgaaaaaagt cagaggatat
    28321 ttttgatggg cttaactgaa ttgtcgggtg caacaatgcg gtaatgtcat tgtaggtcgc
    28381 cgctggattg agccaagatt cccactgatc cttggtaagt atgactggct gacgattatg
    28441 gataatttta agcgacgcca ctgctggaca agtaataata gtgcaactct caaccgtttg
    28501 acgaccattt gtccaggttt cgtacaaccc ggctaaaccg ataacttcct cgccctctat
    28561 ttcaactttg tatggtattt tcttcgcttt tggtttacct acaacttcta gcgtttgaaa
    28621 ttcataaaac caagtggcag gaatgagaca acgttggtgg tgcgaccagg ccgaccgata
    28681 cgtcggcttg gtcgcaatat cctctactcg tgcattaaac gtgctaaact tcattttcct
    28741 cggatcttcc ttccaccatc gtggaatcaa cgaccaatac cgcataacca atttatttgg
    28801 cgagtttttt gaaaccgttg gaatcaacga ttttggataa aacttgatag actgaatgtc
    28861 tttttcgttt ttcggcatgt ttgtcaactc aaacaaatct gcaatttgct ttgcactcat
    28921 gccagtggaa aacgcaaaaa aaccacacat gctattgcaa aaccttcatt tctgttggaa
    28981 cactgcctgt aaagtttagt ttaccgcccg taaaatctgc ctgttgaacg tccaaccctg
    29041 gcacgttgtt gattatatgg ttagccacac tcacaaaagt gccactagca tcaattttat
    29101 ctaagggaac tggtagcctt ccgacttcca aataatcaat ctgagcagtc acctgattat
    29161 ttgtgacact tccagtccct ttgccataca caactggatt aactgtaaga actcccaaat
    29221 aatcaaggcc cttttctaca tcagctttgg aatattgagt gccaccaact tgagcaataa
    29281 atccatccaa gcgatccatt cgcaaccggc cagccacttc aactgtaccg tcaggtgaaa
    29341 acttgacttg gacttgatca atcggcatat atttccaagt actaagattc aacctcccag
    29401 ttacttcttc ttgactgaaa gaagtagtta gtaactccga accagagtaa acaatggatt
    29461 ttccttcaac tggagcatca agagtcttca gtgttgtatc cgctttggct aagtattgat
    29521 catagttttc ttgagttgcc actactccca aatccctggg tttatctgag ccaaacaagc
    29581 tcgatattac aggtacaaat cctaaatacc ctaaaaccaa cactgtacca acaactataa
    29641 gggcagttat tccaaataga aaaaggaaaa tttttttcat attgaattta ttatctctcg
    29701 aagtagtcag atgtcaaata actagtagaa tgatacaatc tcgtccaaca tgaatatctt
    29761 aattactgga cacaaaggtt ttgtgggcag acattttgtc aaatattttt caacaaattc
    29821 aaaaaataac attgttggca tcgatattgt agataatcaa gattgtcgtg atttttttaa
    29881 aactgatgtc agtagcaaag gtcattggga tttagtaatt catttggcgg cgattgtggg
    29941 cggacggcaa acgattgagt ctgacccaat tgctgtggca attgacttga gcattgacgc
    30001 agaacttttc aactgggccg tccgccaacg accaaaaaga attgtatatt tttctagcag
    30061 cgccgcgtat ccaattgcac tccaaaccaa aacgcggcgc cagaaactct cagaatctga
    30121 cattaaccta gctaaaatcg cccaaccaga cctcacctat ggctggacca aactaactgg
    30181 cgaattatta gctcagtttg ctcgcgacca gtatggttta catgtttctg ttctccgtcc
    30241 tttctctggc tatggtgaag atcaagattt gagctatccc tttccctcat ttattcagcg
    30301 tggcaaacaa aaacaaaatc cttttgtgat ctggggagat ggtacccaaa ccagagactt
    30361 tattcatatc gaagacattg ttgccgcaac tttaaaaaca cttgaattaa acctccacca
    30421 acctatcaat cttggctggg gcagacccac ttcattcaat caactcgcca aaatggtgat
    30481 gaaaacagcg ggttactctg ccaagctctg tcatcagcta gacaaaccaa tcggagttca
    30541 ttaccgagtc gctcatccaa aacttttatt ttcgttttat caacctaaaa tttctttaga
    30601 agaaggcatc gcgcgggctc tccatgtcta accctcatgt ctccgccatc tgccccattc
    30661 gagatgagca agcatcaatt ttgaatattt tcgaaacttt caaacctcta ggtggcaaaa
    30721 ccgaattaat ttttgtcgaa ggcggatctc acgattattc ttggtatctt gtgcaaagac
    30781 ttcacaaaca aaccaaccaa tatggagtcc attttcgcgc ctatcaacaa atcggtagtg
    30841 gcaaggcatc ggcagttgct actggatttg accaagctcg aggaaactat ttaatcatag
    30901 tggatgccga tatgaccgtg ccacaattag aactgcccaa aattctcagc ttgttttcgc
    30961 agtacggcga tagaatttta gcctctggca atcgcttgcg tggcttaccc aaaccaaagt
    31021 cattctattg gattaattat ctgggcaact atttttttcg gtactactac tcttttattt
    31081 tagaaacaca gatacaggat atctcctgcg gatctaaagc actcacgagc agatcttggc
    31141 aaaaacttaa aaaaactcgt ttggcagaaa aatcacttga tagttggggt gacatcgact
    31201 ggctgtatta tggtagaaaa attggtctaa acccaaagtt tgttcaggtt gactatgttg
    31261 aacgcgtcac tggtcaatca aaattacaaa atcttgtcac ccgctggcaa tttgctttaa
    31321 aaatgtttac tcttggctta tacattctga aaaatcgaaa ttggcatgaa actaaccaaa
    31381 ctaattcata gacacgaaaa aaaaattgaa acagcgtttc tcgcgatttt tttcactctt
    31441 ttggtaatta aattaacaat agtttttttc caaaatcttg cttcgccgcc actaactacc
    31501 gattcttggg cgtactatag tatttcaaaa actatttttg aagattttgg ttttaccaat
    31561 tatattcgtc agtttgaata tatctccgat tacaatattt cattcccacc cctcttccca
    31621 ttagccattc gattcagtaa ttcaatcatt gatttgggga tttatgcagg atatctactc
    31681 aactttggat ttgcagtagc aattggcatt ttgttgccaa aactaacaaa actaataact
    31741 ggcagtgcat tacctggaat ttttttacta ctactgttac tggtaaacta tcattttctc
    31801 actgaactac tgggtgctcg cagcatgatt ctttcattac ttctattgat tggtttaatc
    31861 catatcttct tgcaaaaaca gttgacccaa ggacacattg ctgcaatagg agctctggca
    31921 ggattactca cacttaatcg attcgatttt ttactaccct ctatcttact cggaatcgtg
    31981 ctgttatttg ttgctcaatt aaccaaaatt cataccggct taatctactc cacagttttt
    32041 ttactggtaa tttcgccttg gatttggtac tcatacagcc attttggcag ttggtttgta
    32101 tctgataatt ctcgcagtat tttgttggct gccaaaacct ttacctttga ctactacccc
    32161 aacccagaac aaatacctac cctttttact caaccattct attggtttgc ctatcgcgtt
    32221 attttcaaag gtgttttctc gttcatccaa tttatgaaag atgtttatta caatccattt
    32281 tttcatcagt tatttattgg cgtgggaatt ttattgacca ccattgcttt cgttattagt
    32341 caaagtggaa aaattattcg ttcaaagtta aatttcgaac ccatacgacg gctcgctttg
    32401 ctcgccccaa tttttctgct tcagcttttc agcattggtt taaccggttt tttggatatg
    32461 cgctatacct tgtttttttt gttttatatt tgcctattct tagttggatt ggtttacttg
    32521 ttactcagca ctcttttaaa accaatgtat gtccttatct tttgtctagt atttttgacc
    32581 atactattct ttgaacaaat tatcttcagt attccctcag actttttgca gttagcacaa
    32641 caacccctta atttgaactc acagctactc aaaccgactg gcgaatatct gcaagtgaat
    32701 gaagcaattt cccagttaga tcaaacaccc attctattgg ttgatacaaa tcaagttgca
    32761 accctttctg cagcgcagtt tgctggtttg actaacatta aaacaattga aacaccaagt
    32821 aatttaaacg atgatgtgtt tctttctttg attaacaact acaaaatcaa ctttgtgtat
    32881 agtaactgtc caaaatggct tgagtctttg caaaatcact ttgtcattac caaaacaaat
    32941 ctaccaaagt tgttacgtgt ttcggttgct accgattcgg caacatttac taatttggag
    33001 tatttaacgg ttcgagacga agcatattat caatgtaata cgtcatcatc aatcgattta
    33061 atcaaatagg agaaattttt tctaactgcg aattctttaa cgattcttct gatcaattgt
    33121 ttagattttt tttcattcac ttccttggtt acatcccaat cgatgcctgg tccaatattt
    33181 ccctcaacta aataaattct acctgcgtta ctcacggcaa aatctagggc gtaaagcgct
    33241 tgtggtttat tgatttttct gacaattttt ttggcgatcg catgaattac ctctggcaca
    33301 tcactggtgg caatatacac taattgacca ccctggtgct cattgcaacg aaaatcatct
    33361 gtttttgcca ttcgcaaata gcattggaag agctgattat gatgaaacag taatctgata
    33421 tcggtcgaag ctctcagtcc attgtactca aaaccttgat caaacaataa aaatggctgt
    33481 aacacaaact cgatggttgg gttctgcgcc ataattgtgt gaatgacttg aacaaagtcg
    33541 gctttaattt ggtacacatg attgccacca gcgccatcgg tagtaagatc aacatccaaa
    33601 acatagcctt caacacctgg ggtaagagta tctggagagt cattgactgt accagttgtc
    33661 gcgatagtat ctccagaagc tgctgagtta agtgcggtgt ttgcacttaa agcccaagcg
    33721 ctccaaccac tagcggcatt agtggtgacg gtaattgtag taccagttcc agaaacaaca
    33781 gagccaacag ctagagcaag attttgagca ttattgttaa acacaaaagt aaaggttgga
    33841 gccacggtag cagtgatgac gatttggtcg ttggcaatga tggggactgc tacatcctta
    33901 gtgtcagcgg tagcggcatc agagccagtt cgagtaaaaa ttgtatgtac aattgtagtg
    33961 gatgaggtag gattgctgat aaggccagta ccaccattgg tgataaaaaa gccatacgta
    34021 gtacttactg ttaaatcagt gactggaaat cttatggtgt ttccagttac attgttggcg
    34081 gtgccaatgc ccggcatagc ggttaccccc tgcgggtaaa ccggctgtgg atacggtata
    34141 gtcagtcgcc acaacgctgt tactccacgc c
//
LOCUS       DAOQZP010000002        29840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_1822_length_29840_cov_5.606816, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000002 DAOQZP010000000
VERSION     DAOQZP010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 29840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29840
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1822_length_29840_cov_5.606816"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1412
                     /gene="dnaA"
                     /locus_tag="PKH60_00160"
     CDS             <1..1412
                     /gene="dnaA"
                     /locus_tag="PKH60_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNQ16495.1"
                     /translation="SKLESQLSPAVFNTWILANPLTQVVDQNSTLIATITSPTAFHST
                     NLKRNLNQQLTDAISQVVGKSVVIQYEIGDQLEKQGAEKNSWSTIKSNVGGNSFTPSS
                     TNSPLVTDLFSDATIKQSEEDRSKFRAKQVGLNPDFVFASFAVSSSNEMAHAAAIAVS
                     KNPGSSYNPLFLYGSVGVGKTHLMHAIGNNLVAQNPNINMLYCTGEDFTNEIVQAIQM
                     KQAASFKRRYRAAQVLLIDDIQFIAGKNTVQEEFFHTFNALIQQRSQIILTSDRPPQS
                     ISLLEDRLRSRFEAGLMIDIGQPTFELRTAILLLKAEQAHLNFPIGLAKQVAEVITSA
                     RKIEGFITNIRSEIELKHKELNEELILSLINQNENERPPKMLTNPSDVIKEVARFYQL
                     KQSELKGKQRIKHLVDARHLAMYIMKEDLQLPLVEIGRWFAGRDHTSVIHAVRKISKD
                     LSFNSRLQQELQQIKNRLV"
     gene            1628..2734
                     /gene="dnaN"
                     /locus_tag="PKH60_00165"
     CDS             1628..2734
                     /gene="dnaN"
                     /locus_tag="PKH60_00165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNQ16496.1"
                     /translation="MKLTIKQTSLQKVLRVVQKSVSTKPQLPILSCILLKATKDSLFL
                     YSTDLYTGVMVEIDATIKEEGSFAVPGKYFTQVINSISSPTIDMEVKDNSLLIHSKES
                     ETKLVGLNPEDFPSFPEISSKNKLIPVKDFQEALASVAFSSSTDQTRPTLTGILLSFE
                     SEKTQLIATDGFRLSIRSIDVEIEKEGTILIPSRPLVDIFKTAEEEKVSSISLSISEE
                     LKQAKFEVGSFSFYIRLLDGEYPPYKKIIPTQIKTFVSCNKNDLEEKLHQALIFNQDN
                     SFITQFKISLTGVTLFSSSPSAGENTSELSGATIKGEEVTIAFNTRYLLDFIKVISDE
                     WVEIEIVESLRPARLKVASLPDYQYVVMPFRVTS"
     gene            2758..2895
                     /gene="rpmH"
                     /locus_tag="PKH60_00170"
     CDS             2758..2895
                     /gene="rpmH"
                     /locus_tag="PKH60_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014245960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNQ16497.1"
                     /translation="MSKRTWQPKKQKRQKKHGFLSRMRTLAGRNLLKRRRTKGRKSLS
                     V"
     gene            2914..3111
                     /gene="yidD"
                     /locus_tag="PKH60_00175"
     CDS             2914..3111
                     /gene="yidD"
                     /locus_tag="PKH60_00175"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNQ16498.1"
                     /translation="MKKQTLLFLQSFGKTLRVSLLQGIFGYTSQCKHSPTCGDYCFKM
                     MSEHGVVKGLTLGFRRLATCW"
     gene            3131..3883
                     /locus_tag="PKH60_00180"
     CDS             3131..3883
                     /locus_tag="PKH60_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HNQ16499.1"
                     /translation="MMENFNFYFSLLLAYLSQLTGNLGLGITVFTIGLRMLLFPITLN
                     SLKAARKMRLVQPELSKLQKIHKGDAQGLQKAQLKLYQDNKINPLAGCLPQILQLVLL
                     ILLYQGLIHFFSLDQVGGYVINPSFLWLNLGEPDRLFILPVLAGLSQFGLSLLMMGPT
                     KIEAKTVEKTKSTSKNKSKDDVKKEQEGPDLSSMMQKQMLYVMPFFSAFIALKLPSGL
                     VLYWVVSTAVSALQQLYANKKADADATTITKK"
     gene            3903..4367
                     /locus_tag="PKH60_00185"
     CDS             3903..4367
                     /locus_tag="PKH60_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNQ16500.1"
                     /translation="MDVQQFCQKLLEHAGIVPTEITSTDSEKELVITIQVSDEEAGLL
                     VGRHGETLAALQRLARVLYGQMNAEKRLVIDVNDYRARRMDNIRSLLVSAADKVLETK
                     QPTTINTYLSPPERFFVHSTLSEEPKYAELESFSIGEDRGRRIIIQLKENQS"
     gene            4367..4801
                     /locus_tag="PKH60_00190"
     CDS             4367..4801
                     /locus_tag="PKH60_00190"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HNQ16501.1"
                     /translation="MTGDSKQSILSIYTDGGSRGNPGKAATGFVFYLDGEEQKRVGHY
                     IGVQTNNVAEYQAFLSSLIEFLDHKDEFSISEICWFLDSKLVVEQLNGRWRVKEVGLL
                     SLWKECKKLLSTLAVPYTISYIPRENNAVADGVVNQTLDETT"
     gene            4823..7681
                     /locus_tag="PKH60_00195"
     CDS             4823..7681
                     /locus_tag="PKH60_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HNQ16502.1"
                     /translation="MKTALIQEEPVVAIFCQSRKLQSAVVNTLSSASFSSWIFDVEKP
                     SFPTTEIYKSIVLFTGLETTREVASILNTASQVNKNTIVCLPYLPKIQNAPTELQTIW
                     DQWFEKLTNLQTQAISSLTSAQFLLIDGWVDELEKPTLSSINLLFSPISQGSYWQCRS
                     RQFIITTDQIIKIALEYLGRPGVMPSVLVRGEDIDFFAVSNQVVGIYEVTHRTTIQPV
                     FSEPRFEQALPFEVQIQKISSNQQNLPSNIAKQLDSYGKPNRFLTPLPVKVGVVEIRQ
                     TQQKASPPVIIDTKHESSENTQPAPPTSRELKPVEFGDQLERIFSQSRIKEKEHHLTK
                     LQTTEKKAKKKLKHNKVLFIIGSISSGISLAILLMGALFILSVRLASNQLFAVVPTEF
                     LSFTEAGNSQQSLQKTIGLLKPQLRLVGQFVDHPLVNEAQSTIDAYERIRMLQTTEEE
                     RKKLQTTIAEHILGQQPTSIAGVLQDFLRLTQTSYTNLAQLQSLLSTSSESEEDNKDW
                     YSNLKEVVSQRKTSAGYIQSLSPLLPTILGIEDKTRTYAILFLNSSELRPTGGFVQAV
                     ALVTVEDGQITATQTLSSYEIDRRIGGVIEPPQDIQDILKEKSWYFRDSNWDPHHPES
                     AKRASWFIEKAMGVRVDGVISLPTTAFAELLEVTGPLTLPQYNESLTSKNIQERLNFH
                     AEVELTDQLKNYPSAVLDAFFQHILQKSSEEFSAVLAALPTLLTSGEIQFSLISEEEQ
                     ELLQPLRWSGSLVIPVCPSQFSQVPCVVDSVYQVETNVGVNRVNASIKRSVEDKIEFS
                     PNLIKHQRFTKLTNSATSTAWPSGQYSTYVRWYLPLKSQLDEILINGVVVDQATIRRT
                     QEHGLLVLGLVVEIPIQSTKTIQINYHQPIAQPLPFSYFFFDQKQPGSTLDSYRVVIQ
                     QPTGSSVVRLAPNALIENSTLTFEPELGGHLLTAVQFK"
     gene            7749..8441
                     /locus_tag="PKH60_00200"
     CDS             7749..8441
                     /locus_tag="PKH60_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNQ16503.1"
                     /translation="MTDNKITFHVHERKIFGKAAKKLKQAGLIPANIYGLNQESVSIS
                     LDKKMVEKQLKVSETGLVYLELDDQKKPIPVLIDLVERHVLTTDLQHITFKRVSLTEK
                     VVTDVELVLVGENSVPNATVLQVLDTLEVESLPTEIPESIKINISELTEVGQVITIKD
                     LIDRTKLAIVCEEDQLEDPVVLLQEVKEEVEEESTEPQETIITGKGKADEEKDQEAEG
                     GAEGGEASTNAD"
     gene            complement(8445..8780)
                     /locus_tag="PKH60_00205"
     CDS             complement(8445..8780)
                     /locus_tag="PKH60_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16504.1"
                     /translation="MKFIFSLRLLYYIVQTMVGLSLYTLRRYQPLLLSVSMLLIVIFS
                     LLLEAILPSLITTPTPKSYDEPTLGSFSQQQLQELLRDWKTLEKIQPASKAINSQVEN
                     LENSLNPTN"
     gene            complement(8842..>9105)
                     /locus_tag="PKH60_00210"
     CDS             complement(8842..>9105)
                     /locus_tag="PKH60_00210"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNQ16505.1"
                     /translation="VSKQLNSLKPGLAADTLYTEFWHWYCDVEIEKHKNSLLSNQELT
                     AGLITFIKLLHPFIPFVTEAIWQELQLAKLVSPPLITTDSWPT"
     assembly_gap    9106..9115
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9116..11072)
                     /gene="valS"
                     /locus_tag="PKH60_00215"
     CDS             complement(<9116..11072)
                     /gene="valS"
                     /locus_tag="PKH60_00215"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNQ16506.1"
                     /translation="MDTRYDHSQHEGKIAQLWEDAKVFVASPTPKSKKAFSIILPPPN
                     ANDPLHLGHAMYAVEDILIRYHRMLGEDVLWLPGTDHAGIETQYVFEKKLQKEGKSRF
                     QYDRKTLYSMIWDYVQDNSNTAVDQLKRLGFSLDWSRFRFTLDPKVVEYVTKTFLSLH
                     QDGLVYRDLQLVNYCTKCGTSYSDLEVVHKDQISSLYTLAYGPLQVATTRPETKFGDV
                     ALAVNPKDKRYASYIGKSISFEDLTGHRELPVIADELVDPAFGTGVLKVTPAHDSIDF
                     MIGQKHHLPMITTIDLQGKLTGAAVGPYAGLTVAKAREQVVADLKTKGLLINENTKYH
                     NSVGVCYRCSRPIEPLPLPQFFLRVKDSKHNLVENVIKILDKKETKIHGAGREKILRH
                     WLNNLVDWNISRQIVWGIPIPAWYQTDGFEDQITVSFIDSSGKYQTNTLENAIKEHKF
                     SIVSAGLQTISASTSVPYLVGIERPNDDHQYLPETDTFDTWFSSGQWPVVTLKTQDNS
                     EDFNRYYPTTVMETAYDILLFWVMRMLLLCTYLTNQTPFAHVYLHGLIRDSKGQKMSK
                     SKGNVINPLEIVDKYGADSLRLALVIRSTPGQDKSVTESDFVAARNFTNKLWNAARYV
                     LTTTQESKASTRVQQTPFYAHLTTVTQS"
     gene            complement(11614..13413)
                     /locus_tag="PKH60_00220"
     CDS             complement(11614..13413)
                     /locus_tag="PKH60_00220"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HNQ16507.1"
                     /translation="MPRKVQLAFWSIYQQISVFGLIVILLIVTLILTLIATKKILPAN
                     TYINSVAVGLLSPEAALLKIEAQLPPAPPADLQLSSAKATESAHINELGLYPNILAVI
                     AESQQPQLSKPQRFLFALKSLFVSQKYSIRYLFAEDKILTWVTKFAETISTPEVKPRA
                     VLGRPGQLASLLIEPGEPGLSVDVVATTKQIEDNFNHQKLTTAVIYSPTLQPLTGVEI
                     SQSKIRAEKFVGQRLELKNPNLHPSINISDQDLISVLALPDGIKEEELRALVTEIASQ
                     VDQEAVNPIFTYDQTSLKVTEFVPPQTGLSLNEATTYQSIVEAFQKIESLSQIDEKAE
                     KDVITIDLVLTEVEPSVSLESTNSLGIKEKIGFGDSYYDHSIPTRIRNVALAASRVND
                     ILIPPGKEFYFNKTLGDVSRATGFEPAYIIRNGLTELGDGGGVCQVSTTLFRSVLDAG
                     LKVTLRLPHSYRVSYYELDRKPGIDATVYSGNVDFRFINDTPNHILIHGETDSKQLYM
                     YYSIYGTSDGRSTEIKDHVTWGYAPPPPTQYIVDPSLPPGAKKQIDWSAAGIKAKFTN
                     VIYDKFDNIIREDTYTSNYKPWSAKYLVGPGTI"
     gene            13438..15420
                     /locus_tag="PKH60_00225"
     CDS             13438..15420
                     /locus_tag="PKH60_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNQ16508.1"
                     /translation="MPDLPLPKPTIPTAAPPPPSIVPAKPPVVGVNPPSTPLDSAATV
                     PSKPASPVIPASKVSPSDDDNQDSELPPPSALKATPAPSIQKPVDVKPAEMMKEMKPP
                     AVVTALSSVSSNQIPAGNAREPIIAEKSSAQVRAQTPVLTRLGGSTLPGLGQQGVYPA
                     APGNLPPNAPLSPGATKNTPSPQPPLAAYPPKIAQTKRSPLRFLPIIVGGLALLLAVI
                     FGASRLLGGSNTTPVAPSASVPPGAVGQDGQDNNLGNNGGTQQAAVTLEYWGLWEPSE
                     VMETIFKEFESQNPGITVQYVSQSHRDYRERLQTAIASKTGPDLFRFHASWAPMLARE
                     LAPLPANVLTTTDFESAFYPVARAQLTINETIVGLPVMYDGLMLYYNKDIFQTAGIQP
                     PKTWSELRTAAATLTIREGDQIKRAGVALGNVSNVEHFSDILALLMLQNGAKLESPNS
                     PEGRDALLFYTNFLTQDKVWSDIMPSSTVAFARGDVAMMIAPSWRAFEIQAINPSLQF
                     ATSTVPQLANERLTWASYWAEGVNALSTKQDAAWKLLQYMSSNATQKKLYAAQAQVRS
                     FGEPYSRKDLADELAAQPLVSPLLQDAPYARGWYMSSYTHDNGLNDQLIKYYADAVNA
                     IITNKAIQEVLNTLSSGVQQVLTQYNLSSPTSQTAI"
     gene            15433..16368
                     /locus_tag="PKH60_00230"
     CDS             15433..16368
                     /locus_tag="PKH60_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16509.1"
                     /translation="MGRRVLSESSLRARVWLVLAYSAVFEFPLSAEEVFRRLWRFAGE
                     SLPTLAQINTSLQELFSLGVIARKGDWWYLLGQPQASANRQQWTKNSQIKLQQATPII
                     SFLTKLPWVVGVAITGSVAVNSAKPNDDIDFMVVTEPNRLWLTRLLTLIVAMKHGKRR
                     SFAKEEPNSWCFNLWLSTNSLAVPPERRSLYTAYEVCQVRWVFNRDKVIEGFLTQNSW
                     VREIIPNFYCWCEQRAREQKRSPFSFELGPNIIIVVFGSILTMVDWCLSVFQRFYMKH
                     HQTNEVVDYHKAYFHPRDSRAMILDKLKSLTANIL"
     gene            16466..17536
                     /locus_tag="PKH60_00235"
     CDS             16466..17536
                     /locus_tag="PKH60_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16510.1"
                     /translation="MHHEKQSLESDLNLHILAQIRAEEILRLLTWKSNQQQKNISLSS
                     AVLPMLGVRKRLSDVFAQRRGLDNSCADEKDVQIATNLLADLIVAVAADAHFTSRGLA
                     NEFEFGRQLTAGELWITQIKQRGFAINFIDPSPTPISTNNLLVLGLEDWSIVNFENGK
                     VKSKSFTGPWSGLKDSENTSLVAGSSGSPKSVPEVVAIYEKLARTKNTRSGVKYVVLH
                     ARTSSGDMEKVALVAVAEVSSRNVLDATIHKGRHARLNEIAAHTSFLSLQLKVGRLRE
                     KELKIVREATERAKRIVDFSNPNELASLLEILMQIEEFKHMGVGEQAYVLTEWFDAIW
                     LGTGDLGEKNPIGVGFNGRPLA"
     gene            17554..17973
                     /locus_tag="PKH60_00240"
     CDS             17554..17973
                     /locus_tag="PKH60_00240"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNQ16511.1"
                     /translation="MITIILVTGVFDLLHSEHVAFLQNAKDLGGRLVVGIESDSRVRK
                     LKGEGRPVQSALERKKALIALEFVDEVIILPNQFETESDHLHLLKLVQPDILAVSSHT
                     PHLDVKRRLMKQVGGKVVVVRNHNPNISTTKILQKNQ"
     gene            18043..19449
                     /gene="radA"
                     /locus_tag="PKH60_00245"
     CDS             18043..19449
                     /gene="radA"
                     /locus_tag="PKH60_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNQ16512.1"
                     /translation="MSKSTSVYICQNCGWQSPRWAGQCASCNSWNSLIEEIQTTTSKQ
                     KKSQSELAVTSFNQIEDWQNGERVSTTIAELDRVLGGDTKCGLVPGEVILLGGEPGIG
                     KSTLLTQVAMGMITNSTQKSAKYEDIVYVAGEESPQQVGLRIRRLLASRHNNKKQTKD
                     EDWNTKLDKLKFITSTDVDELVVHLSNHPPKLVIVDSIQTLVTQDLSGSAGSIGQVRE
                     VTDRLIGVAKSHHIPMFLVGHVTKDGKLAGPMVLEHLVDAVLELKGEKTSELRILRSQ
                     KNRFGATDEVGVFRMTDQGYQEVSNPSEYFLETREKSEPGSAVSCVVEGTRPLLIEVQ
                     ALVIPTYLPQPRRIGRGISANRLQILAAVLEKHAKIPLSRYDVFVSLAGGYESREPSL
                     DLAICVALISSHANKPTPKGSIFIGEVGLLGEVRIAPLLERREKEAMRLGYSSIYSRK
                     LTKTIVQLLAKIATPSKT"
     gene            19977..20777
                     /locus_tag="PKH60_00250"
     CDS             19977..20777
                     /locus_tag="PKH60_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16513.1"
                     /translation="MPDSLLSTIRIRQSYTRELLLGVHLSTVSSISILKNLRRGATNE
                     SISLLTKLSGKLALIITVFVIVGLTLSAIFFLPQIYYRIFPAQVVVTETAQQGTVLGG
                     DFTKPTAKSIGRQYEPPVDESLPEGDWVIIPQIGVRTQFKASSDPNEALVEGAWLSPE
                     YGRPGDTGVPIIIAAHRFGWQWWWQSDYGKYHSFYYLPDTLPGTQVEIISGKRKWLYE
                     IYAGEENNEISDYHADLILYTCKHISSPIRFVRYARLIDPTANSQVGV"
     gene            21041..22426
                     /gene="rho"
                     /locus_tag="PKH60_00255"
     CDS             21041..22426
                     /gene="rho"
                     /locus_tag="PKH60_00255"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNQ16514.1"
                     /translation="MSDSPRPSLPTLTQRKVPAVVPLKRKAAPVISQSNPVDSVPIDS
                     SPNESSPVLVPVVEEKASDATVEDKGKPTEPKNHVQPTNSGPTDPIISGVIDLSRDGH
                     GIVRIGFGDDDGDAYISNAHIRRLRLQSGDMITGPARRPKDGERYWGLLQVDSVNGMS
                     VRDWQQVDRVKFNKLTPVYPDSHIKLEIGEEPLSLRIIDLVAPIGKGQRSLIVSPPKA
                     GKTNLLKDVAAGVAQNHPEIHVMAVLVGERPEEVTDIRRHIATITKGKGEVAASNFDE
                     SAKDQCRVAELALERAKRLVEMGRDVLILLDSITRLARAYNLAMPTSGRTLSGGFDPA
                     ALFPPKKFFGAARNFEIQGSLTIIGTALVDTGSRMDDLIYEEFKGTGNQEVHLLRRLA
                     ERRIFPAIDVQKSGTRRDDLLMDGMTYQSVLTLQRMLDMIDKDDRTTTIIEQLRKTKS
                     NKEFLASLKTG"
     gene            22446..23576
                     /locus_tag="PKH60_00260"
     CDS             22446..23576
                     /locus_tag="PKH60_00260"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNQ16515.1"
                     /translation="MQRRNKNHVQLPKPLTEIFEYLGFLRWYFKARGYRWFARFERIK
                     DSLVDLLYQKRGRYTRPFLHLASLSFVFIALTIGPMLFNTVEENGQQEDLRGISITQA
                     YGPNFYTQQAEEVQQYRGGEIITHYVQEGETLESIAQRYSLQVETILWENDLLKTAPI
                     KPGQELRILPIDGVRHKVSKGETIYSVGKKYGLEGSQIQMLVDYPFNEFLNDETFELA
                     TGQFIMVPEGIKPEEKKVIPNRSAGYLAQTPNAGSVAAQGSFVWPASGMITQRYSFYH
                     KAIDIANRSAGSILAADSGKVIGAGWLDNSGYGNRVIIDHGNGYVTLYAHLRTVQVSQ
                     GQTVNRGDVLGQMGSTGRSTGTHLHFEIRQGGVLLNPQNFLK"
     gene            23580..24764
                     /gene="obgE"
                     /locus_tag="PKH60_00265"
     CDS             23580..24764
                     /gene="obgE"
                     /locus_tag="PKH60_00265"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNQ16516.1"
                     /translation="MQDLIQLLCKAGNGGRGKISFRREKYVPKGGPDGGDGGNGGDVL
                     VVATRHRATLEHLAGKTSIEAKHGAAGGPRKKHGFNSDAVIIEVPLGTRITLSSQNQS
                     ALHRQLICGDLPIKRGDVRFVQYEVDFHGTQITDVDWSEAEKLIPVDQIDVMEPPITG
                     QTIADLVEDGQSILLCQGGFGGKGNTHFKSSVVTTPRKAEYGTPGEARTVWFELRVLA
                     DIGLIGMPSVGKSTLLSVLTRATPKIAAYPFTTLEPQLGRLVLGEKEVVVADIPGLIE
                     GASHGKGLGTDFLRHIAHCQALCYVVAVPEEVLLAQESTEKDIAQLLWQQYQLVKQEV
                     VTAHPELASKQSLVVLNKCDIYSEVMIKTIKQAFAKNKTPLTAVSGATHFGLSELKTQ
                     LS"
     gene            complement(24770..26887)
                     /locus_tag="PKH60_00270"
     CDS             complement(24770..26887)
                     /locus_tag="PKH60_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16517.1"
                     /translation="MLNTNTKDYLATFALGITMSLIFFFGLLVVPITDGFVTDTKSFL
                     LLFGAIILGLIYVGRALIHKGFQMVSSPVLLPTAIFGIVTAVSAVVAKPYPVEELMGV
                     GGSWIAWMIIILIGGALLPKNAGKYVVPALAGTGVAITLLAILQLVGFGPAQLLNRAF
                     GLTIPSSLAFSLTSSTLTAIQILVIAATALVGKIISERLRSPINIALLAIVGVGIVLH
                     LWAVRPNGPAPIVLPSFSASWSIMLDSIRSPRTAMIGSGPSSYINNYRIYKPVFVNGT
                     ENWSAIFTSAVNTPITLLSTVGIIGFLAWALVTYQIVREARAESKNQLPLSLAILTTV
                     FWFILLPASPVLIGLQALLVVALLAGSHSRPKFHLTALIDKVVLNPMNFGVDGMASTK
                     KAPALPVYALAIVFAVVLLISFYGVGRAYYALVLSHQATQASLENDALGLYNNQQAAV
                     RALPYLDSVRRTYAITNLLIASGLSNSEEVTDEQKQQISTLLQQAVTEARAAAYLDPN
                     DATNWQVLAQVYQNMIGIAEDAEQWATQTYVSAIQTSPSDPQLRLALGGVLLNAKQNE
                     QALSVFQQAVNVKPDYANSHYNVAQAYLALEMYDAARQEYEAVLTLLDPATEDFVKAT
                     KELEELKKVIEETSPAEESTNNNTNQPTETSNASTPSLLDQQLNTPAEQAVQNTTDDQ
                     VAPTLAPTVAPSPAEEVAPTDNP"
     gene            complement(26964..>29840)
                     /locus_tag="PKH60_00275"
     CDS             complement(26964..>29840)
                     /locus_tag="PKH60_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16518.1"
                     /translation="GNVGIGTTSPSALIHGIKTTEQLRLGYDASNYYSTTVSSAGGVT
                     FDAVGISSGFTFSDYVGMAGSGLELSSTTSFSKSSGSGSFSFSTNTGNKGIEFVNSGA
                     TTGTDSGFDYSSGVTTGRGMSITANSLTSGSGIYASSSSLTSGSLMNLVVTGSGAASS
                     SQKVLNISNSGTNGTTETTYGAYVTNTRTGEGGDTNVAGYFSASGAASNYGLIVASGN
                     MGIGTTAPGAMLQLGTAASTLGTLRLTGNTSGYTQIQPSAAAGSWTMTLPTGTGSNGQ
                     QLTTNGSGVTSWTAANSLRTTKNILGTMDASVALDTLVNAQVYRFNYKPGMGTSDSET
                     EYVGIMADEAPWAMHYDGTIVNPVNTLGYAILGVQALNQKNTQLENSLNQVNAMLADY
                     PDLSIDQFGALEVFEADNATYQVVDSTTATPINDRFAAFTRTITAIIETGLTRTKELV
                     VTGRAQIADLSVANLSINGQTLREYVVSIIQENQATNPVIVEVSPTPAPVATASESAW
                     LAEVFQRESTTGEPIVEDLTVTDLLTTQDIDATSVTTESLNVNGQTQLGSLLVSQNAS
                     VAGVMTVNELDANSARIDALEAGMAQLDSVRATTAEFAEATISGTLYAQTIADLDRQI
                     AALLEQPSIMDVITGNIPSPQDDYSSLYQVLGSISSTASQAAQLDQSLANLNLVEDDI
                     VLNANAAFIDRYFKVNGIAYIGDSLGVGNTLRVGSKLEITDTYLSFSAGASTPDEAPV
                     FFIQPSGKGMLALMGDLMILDEAGQVTINGNLYVAGNVSVEGSLLANVIEPLNPGESI
                     KLNLGAPTAGSEASISGEIASPSGQFEVLGDAGTPVATVSAQGNASFSSLSLGRDLLE
                     RPNVAGAATESSQLTQEATQTTGRAILPAGNKELIIRNPYVRRDSLIYLTPLGSTNNQ
                     VLFVKTINAPADESIAQATDERSFTVSLDNTTATDISFTWWIVN"
BASE COUNT         8761 a         5939 c         6736 g         8394 t
ORIGIN      
        1 gctcaaagct ggagagccaa ctttctccag cggtatttaa tacgtggatt ttagctaatc
       61 cccttactca agtggtggat caaaactcaa ctcttattgc caccattact agccccacag
      121 cctttcactc aacgaattta aagaggaacc taaatcaaca actcaccgac gcaatctctc
      181 aagtagttgg aaaaagtgtt gtcatccagt atgaaatagg tgaccaatta gaaaaacaag
      241 gagcagaaaa aaactcgtgg tcaactataa aatcaaacgt tgggggaaat tcgtttactc
      301 caagtagtac taattccccc ctagttacgg atcttttttc tgatgcaaca attaagcaat
      361 ctgaagaaga tcgctcaaaa tttagagcaa aacaggttgg attaaatccc gattttgttt
      421 ttgcctcgtt tgccgtttcc tccagcaacg aaatggctca cgccgccgca attgcggtat
      481 caaaaaatcc tggatcttca tataatcccc tcttccttta tgggtcggtg ggggtgggta
      541 aaacccactt aatgcatgct attggcaata atttggtggc tcaaaaccca aacattaata
      601 tgctgtattg cacaggagaa gattttacaa atgagattgt tcaggcaatt caaatgaaac
      661 aagcggctag ttttaagaga agatatcggg ccgcacaggt gcttcttatt gatgatattc
      721 agtttatagc tggtaaaaac actgttcaag aagaattctt tcatactttt aacgcattaa
      781 tccaacaacg aagtcagata atattaacct cagatagacc ccctcaaagc atctctttac
      841 ttgaggatag attgagatcc cgctttgagg ccggattaat gatagatatt ggccaaccca
      901 cctttgagct tcgcacggca attttacttc ttaaggcaga gcaggcgcat cttaatttcc
      961 caattgggtt ggccaaacaa gttgcagaag tcattacaag tgccagaaaa attgagggat
     1021 ttattacgaa tattcgctca gaaattgaac taaaacacaa agaattaaat gaagagctaa
     1081 ttctttcatt aatcaatcaa aacgaaaatg aaagaccacc aaaaatgctt accaaccctt
     1141 ctgatgtaat taaggaagta gctcgttttt atcaattaaa acaatcagaa ttaaaaggta
     1201 agcagcgtat caaacatcta gtagatgctc gtcacctagc gatgtatatc atgaaagaag
     1261 atttacaact tccgctagtt gaaattggca gatggtttgc tggaagagat catactagtg
     1321 taattcatgc agttcgaaaa atttctaaag acctttcgtt taattcaaga cttcagcaag
     1381 aattacagca aataaaaaac agattggtgt gaaaaactag gttaaaaaga aaggaatttg
     1441 ttgttaataa aaatcgaaga ttgtgaatag gtgttaaaaa ttacaaatgc tgttagtatg
     1501 tgagttatgt ttttcacaat tttatctcga ttacacatta caaattatcc acgcgctttt
     1561 tctagatgaa aacatgtaat ttattcacaa ttagagacta ccctactaga acaactatta
     1621 tttatatatg aaactaacta ttaaacaaac ttctttgcaa aaagtattgc gtgttgtgca
     1681 aaaaagcgtt tcaactaaac cccaattacc aattttatct tgtattttgc taaaggctac
     1741 aaaagattct ttatttctgt attcaacgga tctttatacc ggagtaatgg tggaaataga
     1801 tgccaccatc aaggaagagg ggagctttgc cgttccagga aaatatttta ctcaagttat
     1861 taattcaatt tcttctccaa caatagacat ggaagttaaa gataatagtc ttttaataca
     1921 ttcaaaggaa tctgaaacca aactagttgg actaaatcct gaagactttc cctcatttcc
     1981 cgaaatttcg tctaaaaata aactaatacc agtcaaagat tttcaggaag ctttagcgag
     2041 cgtggcattc agttcttcca ccgatcaaac cagacccacc cttaccggca ttcttttatc
     2101 ctttgaaagc gaaaaaacac aactaatagc gactgatgga tttaggctat caattaggtc
     2161 tattgatgtt gaaatagaaa aagaaggcac tatcttgatt ccatcaagac cactggttga
     2221 tatctttaaa acagcagaag aagaaaaagt ttcgtcaata tcactctcga tatcagaaga
     2281 gttaaagcaa gccaaatttg aagtgggatc tttttctttt tatattcgcc tcctagatgg
     2341 tgaatacccc ccttataaaa aaatcatccc aactcagatt aaaacgtttg tgagttgtaa
     2401 taaaaatgat ttggaggaaa aattacatca agctctaata tttaatcaag ataactcatt
     2461 tattactcag tttaagataa gtttaactgg agttactctt ttttcatcct caccaagcgc
     2521 tggagaaaac acgtctgaac tttctggagc aaccataaaa ggtgaagagg taactattgc
     2581 ttttaatact cgatacttac ttgatttcat taaagtgatc tccgatgagt gggttgaaat
     2641 agaaatagtg gaaagtttgc ggccagcgag actaaaagta gcttcacttc cagactacca
     2701 gtatgttgtc atgccctttc gggtaacctc atgatttatg gtgtataatg cccccttatg
     2761 tcaaaacgaa cgtggcaacc aaaaaaacaa aaaagacaaa aaaaacacgg ctttttatct
     2821 aggatgagaa ccttagctgg tagaaacctg ttaaaacgca ggagaactaa aggaagaaag
     2881 tccctctctg tttaatcgcg ccaaaatagt ttcatgaaaa aacaaacctt attattttta
     2941 caaagttttg gaaaaacact gagggtaagt ctattacagg gaatttttgg ctacacatct
     3001 caatgtaagc actcacccac ttgtggagac tactgcttta aaatgatgag cgaacatgga
     3061 gtagttaaag gattaaccct tggctttcgt cgcctagcaa catgttggta agtggagaga
     3121 attaaaaaat atgatggaaa attttaactt ttatttttct ttgttattag cttatctaag
     3181 tcaactaacc ggcaatttag gcttaggaat tacggttttt actattggtt taagaatgct
     3241 actctttccc attactctta attcgctgaa ggcagcgagg aaaatgaggt tggttcagcc
     3301 agaactctct aaactccaaa aaattcataa aggagatgct cagggattgc aaaaggctca
     3361 actaaagctg tatcaagata ataagattaa tccgctcgcg ggttgtcttc ctcaaatctt
     3421 acaactcgtc ttgttaattc tgttgtatca ggggttaatt cacttctttt cacttgacca
     3481 agttggtggg tatgtcatta atccatcatt tctatggctt aatttgggcg aaccagaccg
     3541 attgtttatt ctcccagtat tagctggcct atctcaattt ggactctctc ttctcatgat
     3601 gggaccaacg aaaatagaag ctaaaacagt cgaaaaaaca aaatcaactt ctaaaaacaa
     3661 atcaaaggat gatgtaaaaa aagaacaaga aggaccggat ctttcatcaa tgatgcaaaa
     3721 acagatgcta tatgttatgc cgttttttag tgcctttatc gctcttaaat taccctcagg
     3781 attagtttta tattgggtgg tttctaccgc agtcagcgct ctccaacaac tttatgccaa
     3841 taaaaaagct gacgctgatg ccaccacaat tactaagaaa tagtagtttt aaggagatta
     3901 caatggacgt ccaacaattt tgtcaaaaat tattagaaca tgccggaata gttccaacag
     3961 aaattacctc tactgattca gaaaaagaat tagtaatcac tattcaggtt tcagacgaag
     4021 aagctggtct tctggttgga aggcatggag aaacacttgc ggcgcttcaa agacttgcga
     4081 gagttttata cggtcaaatg aacgccgaaa aacggctcgt aattgacgta aacgactacc
     4141 gcgcgagacg aatggataac atccgttctt tgcttgtttc agcagctgac aaggtgcttg
     4201 aaacaaagca accaaccacg ataaacacat atctttctcc acccgaacga ttttttgttc
     4261 actcaaccct gtcggaagaa ccaaagtatg ccgagcttga aagtttttct attggtgagg
     4321 atcgtggtcg aagaataatt attcaattga aggagaatca gtcgtgatga ctggtgatag
     4381 taagcaatca attctttcca tatacaccga tggaggctct cggggaaatc caggcaaagc
     4441 agcaacgggc tttgtgtttt atctagatgg tgaagagcaa aagagagttg gtcactatat
     4501 tggggttcaa accaacaatg tggctgaata tcaggccttt ttaagttcgc ttattgagtt
     4561 tcttgatcac aaagatgagt tttctatatc ggagatttgt tggtttcttg actcaaaact
     4621 tgttgtagag caattaaatg gtcggtggcg agtaaaagaa gtaggtttac tatcactgtg
     4681 gaaggagtgt aaaaaactac tttcaacgct agcggttccc tatacaattt cgtatattcc
     4741 aagagaaaac aacgctgttg cagacggagt ggtaaatcaa acacttgatg aaacgaccta
     4801 gaagacggta ggatgcaagt acatgaaaac tgcccttatc caagaagagc cagtggtggc
     4861 gattttttgt caaagccgaa aactacagag tgctgtagta aatacgcttt ctagcgcgag
     4921 cttttctagt tggatttttg atgtagaaaa accctctttc ccaaccacgg aaatttacaa
     4981 atcaatcgta ttgtttactg gtctagaaac caccagagaa gtagcaagta ttttaaatac
     5041 tgcttctcaa gtaaataaaa acaccattgt gtgtctcccc tacttgccaa aaatacaaaa
     5101 tgctcccacg gaacttcaga cgatttggga tcagtggttt gaaaaactaa caaacttaca
     5161 aacccaggct atttcgtctt taacctctgc tcaattttta ctaatcgatg gttgggttga
     5221 cgaattagaa aaaccaacac tttcctctat caacttattg ttctctccga tttctcaggg
     5281 tagttattgg cagtgtcgat ctcggcagtt cattattact accgatcaaa ttattaaaat
     5341 agcattagag tatttgggta gaccgggcgt gatgccatcg gttttagttc ggggagaaga
     5401 cattgacttc tttgctgttt ctaatcaagt tgttggaata tatgaggtaa cgcatcgcac
     5461 tacaattcaa ccagtgtttt ctgaaccaag gtttgagcaa gcgctgcctt ttgaagttca
     5521 aattcaaaaa atatctagca atcaacaaaa tttaccaagc aatattgcaa agcaattaga
     5581 ttcatatggc aagccaaatc gttttttaac cccattaccc gttaaggtgg gggttgtcga
     5641 aatcagacag acgcaacaaa aagcttcgcc acctgtaata attgatacaa aacatgagag
     5701 ctcagagaac acccagccag caccgcccac atctcgggag ctgaaacccg tggagtttgg
     5761 agatcagctt gaaagaattt ttagccagtc gagaattaaa gagaaagaac atcatcttac
     5821 caaattacag acaactgaaa aaaaggcaaa aaaaaagctt aagcacaaca aggtcttatt
     5881 tattattgga tccattagtt ctggaatctc cctcgccata ttgcttatgg gggcgctctt
     5941 tattctctcc gtcagacttg ctagcaatca actatttgcg gttgtgccaa ctgagttttt
     6001 aagtttcacg gaggctggga attctcaaca atcgctccag aaaacaatag gcttattaaa
     6061 accgcagctc cgacttgttg gtcagtttgt ggatcatcct ttagtaaatg aagcacaatc
     6121 taccattgat gcgtatgaaa gaatacggat gcttcaaact actgaggagg aaagaaaaaa
     6181 actacaaaca accatcgccg aacacatctt gggacaacag cccacttcaa ttgctggcgt
     6241 actacaagac tttcttcgtt taacacaaac ttcttacacc aacttggctc agcttcaatc
     6301 tctgctgtca acttctagtg agagcgaaga agacaataaa gattggtact ccaatctaaa
     6361 agaggttgtt agtcaaagga aaaccagcgc tggttatatt cagtcgctct ctcccctact
     6421 cccaactatt ttgggtattg aagacaaaac aagaacctac gccatactct ttttaaactc
     6481 atcggaatta aggcccacgg gtggatttgt acaggctgta gccctagtca cggttgaaga
     6541 tggtcaaatt acagccacac aaactttgag tagttatgaa attgatagac gcattggtgg
     6601 ggtaattgag ccaccacaag atattcaaga tattttaaag gaaaaatctt ggtattttag
     6661 agatagtaat tgggatcccc atcatcctga aagcgcaaaa agagcctctt ggtttataga
     6721 gaaagcaatg ggagttcggg ttgatggtgt gatttcattg ccaaccaccg catttgctga
     6781 gttgttggaa gtaacggggc cactaacgtt gccccaatac aatgaaagcc taacgagtaa
     6841 gaacatacag gagcgcctca actttcatgc cgaggttgag ctgaccgatc agttgaaaaa
     6901 ttatccaagc gctgttttgg atgcattttt ccaacatatt ttgcaaaaat cgagcgaaga
     6961 attttccgct gtactcgccg ctctacccac gcttttgacc tcaggagaaa ttcaatttag
     7021 cctcatttct gaggaagaac aagaattatt acagccactt cgttggtctg ggtcgttggt
     7081 gatcccggtt tgtccatcac aattttctca ggtaccttgt gtagttgatt cggtttatca
     7141 agtagagact aatgtggggg tcaatcgagt aaacgcaagt ataaaaagga gtgttgaaga
     7201 taaaattgaa ttttcaccca acttaattaa gcatcagcgc tttacaaaat tgacaaattc
     7261 ggcaacttct accgcttggc cgagtggaca atattcaacc tatgttcggt ggtatcttcc
     7321 attaaaaagc cagctggatg aaattctcat caatggtgtg gtggttgatc aagcaactat
     7381 taggagaact caagagcatg ggttgctggt gttggggttg gttgttgaga taccgattca
     7441 atcaacaaaa actattcaaa taaattatca tcaaccaatt gctcaacccc tccccttctc
     7501 ctactttttc ttcgatcaga agcaaccggg ttcaacgctt gactcctatc gtgttgtaat
     7561 acaacaacca actggttcgt ctgtggttcg tttggcccca aatgctttaa ttgagaactc
     7621 tactctgact tttgaaccag aattgggagg ccatcttctt actgcagtgc aatttaagta
     7681 atggggcaaa tctacccgca atggaataaa aaagtttgat ataatgctgg cctaattaaa
     7741 ggagtattat gaccgataat aaaattactt ttcacgtgca cgagcgaaaa atctttggta
     7801 aagcggccaa aaagcttaaa caagctggtt tgatacctgc aaatatttat gggcttaatc
     7861 aagagtcagt ttcgattagt ctagacaaga agatggtgga aaagcaactc aaggtctctg
     7921 aaacaggtct tgtatattta gagctagacg atcaaaagaa acccattccg gttcttatag
     7981 acttggtgga gcgccacgta ttgactacag atctacaaca tatcaccttc aaaagagtga
     8041 gcttaacaga aaaagttgtt actgatgttg aactagttct agtgggtgaa aattctgttc
     8101 ctaacgcaac tgttttgcag gttttagata cattggaggt tgaatctctt ccaacagaaa
     8161 ttccagaatc aattaaaatt aatatttccg agttaaccga ggttgggcag gttattacaa
     8221 tcaaggattt aatagatcgc acaaaattgg ccattgtttg tgaggaggat cagttggaag
     8281 atccggtggt tttacttcaa gaagtgaaag aagaggtgga ggaagaatca acagaaccac
     8341 aagaaaccat tattactggc aagggcaaag ctgatgagga gaaggatcag gaagctgagg
     8401 gtggagcaga gggtggtgaa gcctcaacca atgctgacta atctttaatt ggttgggttt
     8461 aacgaatttt ccagattctc tacttgagaa tttatcgctt ttgaagctgg ttgaattttt
     8521 tcgagcgttt tccaatctct tagtagttct tgaagttgtt gttgtgaaaa tgaaccaagc
     8581 gttggttcgt cgtacgattt tggagttggg gtggtaatta gtgatggtaa gattgcttca
     8641 agaagtaacg agaaaataac aatgagaagc attgatacac tcagaagaag gggttggtag
     8701 cgcctcaggg tgtaaagtga taaacccacc atggtttgga caatatagta gagtagtctg
     8761 agcgagaaaa taaatttcac gcccgtagta tagctctttt tatacgagct ttgaaacctc
     8821 tggaaaattt tttagattta gttaagttgg ccaactatct gttgttatta gtggggggga
     8881 tacaagtttg gcgagttgaa gctcttgcca gatggcttct gtgacaaagg ggataaaggg
     8941 gtgaaggagt ttgataaacg tgattaagcc cgcagttaat tcttgattcg aaagcagact
     9001 gtttttgtgt ttttcaattt caacatcgca ataccagtgc caaaactcag tgtatagggt
     9061 gtcggcggca agtcccggct ttaacgagtt aagttgtttc gacacnnnnn nnnnncagac
     9121 tgcgttacag tggttaaatg ggcatagaat ggggtttgtt gaactctggt ggaagcctta
     9181 ctctcttgag tggtggtcaa aacatatcgt gctgcattcc aaagtttatt agtaaagttc
     9241 ctcgcagcga caaagtctga ctcagtaacg cttttgtcct ggcccggtgt agagcggata
     9301 acaagcgcca gtctgagtga gtcggcccca tatttatcga cgatttctaa tggattaatc
     9361 acgttgccct tgctcttgct cattttttga ccttttgagt cgcgaataag accatgaaga
     9421 tagacgtgag caaatggggt ttggtttgtt agataggtgc aaagcaatag catgcgcatt
     9481 acccaaaaaa gcaagatgtc atacgctgtt tccatgacgg ttgtggggta gtagcgatta
     9541 aaatcctcag aattgtcttg ggtctttagt gttacaaccg gccactgtcc agacgaaaac
     9601 cacgtatcaa aagtgtcggt ttctggtaaa tactggtggt cgtcatttgg tcgctcgatg
     9661 ccaactaagt aaggtacgct tgttgaagcg ctgattgttt ggaggccagc ggacactatg
     9721 gaaaacttat gttccttgat ggcattttca agtgtatttg tttggtattt gccgcttgag
     9781 tcgataaacg agacggtgat ttgatcttca aatccgtcgg tttggtacca ggctggaatg
     9841 ggaattcccc agacaatttg gcgactaata ttccagtcaa ctaggttgtt gagccaatga
     9901 cggagtattt tttcacgacc ggctccgtga atctttgttt cttttttgtc tagtatttta
     9961 attacgttct ccaccaagtt gtgcttagag tctttaactc tcaagaaaaa ttggggtagg
    10021 gggaggggct caattggcct gctacatctg tagcatactc ctactgagtt atggtatttt
    10081 gtgttttcat tgattagtaa tccttttgtt tttaaatctg ctaccacttg ttcgcgcgct
    10141 ttagcaacag tgagaccagc gtatggacca acggcagccc cagtcaactt tccctgaaga
    10201 tcaatggtgg taatcatggg taagtggtgc ttctgaccta tcataaaatc aatggaatca
    10261 tgtgctggcg taaccttcag tacaccggtg ccaaaagctg gatcgacaag ctcgtcagca
    10321 attacgggta attcgcgatg cccagtaaga tcttcaaaag agattgattt accaatataa
    10381 ctagcatatc gcttgtcttt tggattgaca gccagtgcga cgtctccaaa tttggtttct
    10441 ggtctagtgg tggcaacttg cagcggcccg taggcgagcg tatagagcga actaatttga
    10501 tccttatgaa ctacttccag atctgaatag ctggtgccac atttggtgca gtagttgacc
    10561 aactgaagat ctcgatacac caaaccatct tggtgaagtg acaggaaggt cttggtaaca
    10621 tactcaacaa cttttgggtc aagcgtaaag cgaaaacgac tccaatcaag gctaaacccc
    10681 agtcttttta gctgatctac cgcggtatta gaattatcct gaacgtagtc ccaaatcatt
    10741 gagtaaaggg tctttctatc gtattgaaat cgacttttac cttctttttg cagttttttt
    10801 tcaaacacat attgtgtttc aatacccgcg tgatctgtac caggcaacca cagcacatct
    10861 tcgcccagca ttctgtgata gcgaattaaa atatcctcta ctgcatacat ggcgtgaccc
    10921 aagtgaagtg ggtcgttagc attgggtggg gggaggatga tactaaaagc tttttttgat
    10981 tttggggtag gggaggccac aaaaactttc gcatcttccc agagctgagc aattttgcct
    11041 tcgtgttgtg agtggtcgta gcgagtgtcc atattccaga ttataccgcg aaaaaaaccg
    11101 actttggtta gtgatatact ataggttgtg ataagtgaga gcgaagtgat gatccgtttt
    11161 ttctttctct cgcgagttcg taaatattta ttgagagtag gggagaacaa gaataagttg
    11221 ggggaaacta ttttaaaaaa tttagagcgt caagagagag cgggttaaaa ggatctagga
    11281 ggcttgaggc atggcaatct tggtgtcaag caatgcacga acaaggttca actatgccct
    11341 taagtatgct tccatgtcca cccccactga actagtgtta ttcacttagc tcagcttcac
    11401 accatatgtg gtaaagcgta tttttataag taaagcgatt cttactaagt aaagtaactc
    11461 aatggtaaat ttacattagt gtgagtcaag tctgtttttg aagctgagtt ttaccaggcg
    11521 tgctttcttg gtggatacac actgactagt aaatacattg ttagatttct cagcagaaac
    11581 acaatgatgc agggatcttt aacttggtgt tgattatatg gttccaggac caactaggta
    11641 tttggcagac caaggtttgt agttagaggt gtaagtatct tcacgaatga tgttgtcaaa
    11701 tttatcataa ataacattgg taaatttggc tttaatgccc gccgccgacc agtctatttg
    11761 ttttttggct cctggcggaa ggcttggatc aactatgtac tgggtgggcg gcggcggtgc
    11821 atatccccag gttacatgat ctttaatttc agttgagcgt ccatcactag tgccatagat
    11881 gctgtagtac atatatagtt gttttgagtc agtttctcca tgaattaaga tatggttggg
    11941 cgtgtcattg ataaatcgaa aatcgacatt gccagagtaa acggtggcat caataccagg
    12001 ttttctgtcg agttcatagt agctaacacg atagctgtgg gggagtctca gagtgacttt
    12061 taagcctgcg tctagaactg atcggaaaag cgttgtgcta acctgacata cacctccgcc
    12121 atcaccaagc tcggttagtc cattgcgaat aatataggcg ggctcgaacc cggtggctcg
    12181 ggaaacatct cctaaggttt tgttgaaata aaactcttta cccggtggga taagtatgtc
    12241 attcactcta ctcgcagcca acgccacatt gcgaattctg gtgggaattg agtgatcgta
    12301 gtaactatct ccgaatccta ttttttcttt tattcccaat gagttggtag actctaggct
    12361 aactgacggc tcaacttctg taagtactaa atcaatagta attacatctt tctctgcctt
    12421 ttcgtcaatc tgtgacaagc tctcaatttt ttgaaatgcc tcaacaatac tttgataggt
    12481 tgtagcctcg ttgagggata atccagtttg gggtggaaca aactcagtaa ctttgagact
    12541 tgtttgatcg taagtaaaga tgggattaac cgcttcttgg tcgacttgag atgcaatttc
    12601 tgtcacgagc gccctcagtt cttcctcttt aattccatct ggaagcgcta agactgaaat
    12661 gagatcttgg tctgaaatgt tgatgctggg gtgtaaattg gggtttttca gctccaatct
    12721 ttgaccaacg aatttctctg ctctaatttt tgactgtgaa atttcaacac cagtgagggg
    12781 ttgaagggtg ggggagtaaa ttactgcagt tgtcaacttt tgatgattga aattgtcttc
    12841 aatctgtttg gtagtagcaa ccacatctac ggataatccg ggttctcctg gctcaatgag
    12901 aaggctggcg agttgaccag gtctaccaag tacggctctc ggtttaacct caggagtact
    12961 gatggtttct gcaaattttg taacccaagt aagaattttg tcttcggcaa acagatatcg
    13021 aatagaatat ttttgggaaa caaagagtga cttgagcgca aagagaaatc tctgcggctt
    13081 gctcagttgt ggctgttggg attccgcaat taccgccaat atatttggat acagtccaag
    13141 ttcattaatg tgcgcgcttt cagttgcttt ggccgaactg agttgcaagt ctgctggtgg
    13201 agcgggtggt aactgcgctt caatttttaa gagtgcggct tctggggaca acaatccaac
    13261 agctacacta tttatatatg tgttagctgg caagatcttt ttagttgcaa ttaacgtaag
    13321 tattagggtc acaatcagta aaataactat caaaccaaaa acactgattt gttggtagat
    13381 actccaaaaa gctaattgca ctttacgcgg catgtttatg ctattataga gtggtagatg
    13441 ccagatttac cactgcctaa gccaaccatc ccaaccgcag cgccaccgcc gccgtcaatt
    13501 gtgccagcaa aacctccagt tgttggggta aatccgcctt ccacaccttt ggattcagcg
    13561 gcaacagttc caagcaaacc tgcatcgccg gtaattccag catccaaagt aagtccatcc
    13621 gatgacgata atcaagactc tgaattgcct ccaccttcgg cgttaaaagc gactcccgcg
    13681 ccctcgattc aaaagccagt tgatgtaaaa ccagctgaaa tgatgaagga aatgaagcca
    13741 cctgcggtgg ttaccgcact ttcttctgtt tcaagtaatc aaattccggc agggaatgcg
    13801 cgggagccga taatcgccga aaaatcttca gctcaggtga gggctcagac tcctgtttta
    13861 actaggttgg gtggctcaac attaccagga ttgggtcaac agggtgttta tccagctgct
    13921 cctgggaatc ttccacctaa cgcacctctt tcgcctggtg ccacaaaaaa tactccatca
    13981 ccacaaccgc cacttgcagc atatccacca aaaattgctc aaacaaaacg atcgccgctt
    14041 cgattcctac ccattattgt tggtgggcta gccctattac tcgctgtgat ttttggtgca
    14101 tctcgtttac ttggtggatc taatacaaca ccagtagcgc cgagcgcatc ggtgcctcca
    14161 ggtgccgtgg gtcaggatgg tcaggacaat aacttgggta ataacggagg aactcaacaa
    14221 gcggctgtaa ctttggaata ttgggggctg tgggagccaa gtgaagtaat ggagactatt
    14281 tttaaagaat ttgaatcaca aaatccaggc atcacggttc aatatgtcag tcagtcacat
    14341 cgagattacc gcgaaagatt gcaaacagct atcgcttcaa aaacaggccc agatttgttt
    14401 cggtttcatg cctcatgggc tcccatgttg gctcgtgagc tagctccact acctgcaaac
    14461 gtattaacca ctactgactt tgaatcagct ttttatccgg tggcgagagc tcagttgact
    14521 ataaacgaaa ccatagtcgg tttgccagtg atgtatgacg gactcatgtt gtattacaat
    14581 aaagacatat tccaaaccgc tgggatccag cccccgaaaa cttggtcaga gctacgtact
    14641 gcagccgcca cgctaactat acgtgagggt gatcaaataa aacgagcggg ggtggcgttg
    14701 ggcaatgttt caaatgtgga gcacttctct gacatattgg ctttactgat gctccaaaat
    14761 ggagcaaaat tggagtcacc taattcccca gagggaagag atgcgctttt gttctataca
    14821 aacttcctca ctcaggacaa agtttggagt gatattatgc cttcttcaac cgtggcattt
    14881 gctcgcggcg atgtggcgat gatgattgct ccctcttgga gggcgtttga aattcaagca
    14941 ataaatccaa gcctgcaatt tgcaacctct actgtgccac aattggcgaa tgaacgactg
    15001 acttgggcaa gttattgggc agaaggtgta aatgcgctta gcaccaagca ggatgctgcc
    15061 tggaaactat tgcaatatat gtcatcaaac gccacacaaa aaaaactcta cgccgcccag
    15121 gctcaggtga gaagctttgg cgaaccctat tctcgtaaag atttggctga tgaacttgcc
    15181 gcgcagccac tggtgtctcc actattgcag gatgcccctt acgctagagg ttggtatatg
    15241 agtagttaca ctcatgacaa tggtttaaat gatcaattaa ttaaatatta tgctgatgcc
    15301 gtaaacgcta ttatcacaaa taaagccatc caagaagtgc tcaacaccct cagctctggt
    15361 gttcaacaag tattgactca gtataatttg tcatccccaa cgtctcaaac agctatataa
    15421 tctgtcatac ctatgggcag gagagtattg agtgagtcta gtttgcgtgc gcgagtttgg
    15481 ttggttttgg cttactctgc agtattcgag tttccgctgt ctgcagaaga ggtctttcgc
    15541 cggctttggc gatttgctgg tgaatcactt cccacattgg cgcaaattaa tacttcgctt
    15601 caagaattat tttcccttgg agttattgct agaaaaggtg actggtggta tctattaggt
    15661 cagccccagg cttcagcgaa tcgccaacaa tggaccaaaa attcgcagat taaacttcag
    15721 caagctactc caattatttc atttcttacc aaattaccct gggttgttgg ggtcgcaatt
    15781 acaggatcag ttgcagttaa ttctgcaaaa ccaaacgacg acatcgattt tatggttgta
    15841 accgaaccaa atagactatg gttgactcgc ttacttactc taattgtggc tatgaagcac
    15901 ggcaagcgta ggtcttttgc taaagaagaa ccgaatagtt ggtgtttcaa tttgtggctt
    15961 tcaacgaatt cgcttgccgt ccccccagag cgtcgatcac tctacactgc ttacgaagtc
    16021 tgtcaagttc gctgggtatt taatcgggac aaggtgattg agggtttttt aactcaaaat
    16081 agttgggtta gggaaattat tccgaacttt tattgttggt gtgaacaacg cgctcgagag
    16141 cagaaaagaa gccccttttc atttgaattg ggtccgaata ttattattgt tgtgtttggc
    16201 tcaattttga caatggttga ttggtgccta agcgtgtttc agaggttcta tatgaagcac
    16261 catcaaacca atgaagtagt tgattatcac aaagcatatt ttcatccgag agatagtagg
    16321 gcgatgatat tggataagct caaaagctta actgccaaca tattataaat tgagaaagta
    16381 ttctacttct acaaactttt cacaaattga tttataatca catcaataat aatcaataag
    16441 agtcaaatag atagaaaatt ttcagatgca ccacgagaaa caatctcttg aaagtgattt
    16501 aaacctccac attttagccc aaataagggc agaagagata cttagactcc taacatggaa
    16561 atcaaatcaa caacaaaaaa acattagctt gagctcggct gtattaccca tgttgggagt
    16621 aaggaaaaga ttaagtgatg tttttgcaca acggagggga cttgataatt cgtgcgcaga
    16681 tgaaaaagat gttcaaattg ccacaaattt attagctgat ttaattgtgg cagttgccgc
    16741 agatgcgcat ttcacatcaa ggggattagc aaatgaattt gaatttggta gacaactaac
    16801 tgccggagaa ctgtggatta cacaaattaa gcaaagaggt tttgcaatca attttataga
    16861 tccgtctcct actccaataa gcaccaacaa cttactagtt cttggtcttg aagattggtc
    16921 tatagttaat tttgagaatg gaaaagtcaa gtcaaaatca tttactggtc cttggagtgg
    16981 gctaaaagat tcagaaaaca cttctctagt tgctggatcc agtgggagcc caaaaagtgt
    17041 tcctgaggta gtagctattt atgaaaaact tgctcgcact aaaaatacaa gatctggtgt
    17101 gaagtatgta gtgctgcatg cgcgcactag ttctggagac atggaaaaag ttgctcttgt
    17161 ggccgtcgct gaggtgtcgt ccagaaatgt attagatgca actattcata aagggagaca
    17221 tgcgagacta aatgagattg cagctcacac atcatttttg tcattacaac ttaaagttgg
    17281 tcgcctgagg gaaaaagagc ttaaaattgt tagagaagca actgaaagag caaaaaggat
    17341 agttgatttt agcaatccaa atgagttggc atctttatta gaaatcctca tgcaaataga
    17401 ggagtttaaa cacatgggcg ttggtgagca agcttatgtt ttaacagaat ggtttgatgc
    17461 catatggcta ggtactggcg atcttggtga aaaaaatcca attggagttg gtttcaacgg
    17521 taggccgttg gcttgataca atcaagttaa tttgtgataa ccatcattct tgtaaccggc
    17581 gtttttgacc tactccattc agagcatgtt gcgtttttac agaacgccaa agatttggga
    17641 ggtcgactag tcgtggggat cgaatctgat tcaagggtga ggaagctgaa gggcgagggg
    17701 aggccggtac aatcggcgct ggagcgaaaa aaagcactca tagctttgga gtttgttgac
    17761 gaggtgataa tcttacccaa tcagtttgaa acagaatccg accatcttca cttgttgaaa
    17821 ttagttcaac ccgatatatt ggccgtgtcg tctcacacgc cacatttgga tgtgaaacga
    17881 cggctgatga agcaggtcgg tggaaaggtt gtggtagtaa gaaatcataa ccccaacatt
    17941 tccaccacaa aaatactaca aaaaaatcaa tgatcataat aattactcta ttaattgatt
    18001 actacttgaa ttataaacat ttactacaag tgaacaaata ctatgtctaa atcaacatcc
    18061 gtatatattt gtcaaaattg tggttggcaa tcaccgcgat gggccggtca gtgtgcttca
    18121 tgtaatagct ggaatagctt gatagaagag atccaaacca ctacttcaaa gcagaaaaaa
    18181 tctcaatcag agcttgctgt tacctcattt aatcaaattg aggactggca aaatggtgaa
    18241 cgcgttagta ccaccattgc ggaactagat agggtgcttg gaggtgatac taaatgtggt
    18301 ttggtaccag gggaggttat tttgttgggt ggagagccag gaatcggcaa atcaaccctg
    18361 cttactcaag tagccatggg aatgatcaca aatagtactc aaaaaagtgc aaaatatgag
    18421 gatattgtgt atgttgctgg tgaagagagc ccacaacaag ttgggctcag aattcgccga
    18481 ttactcgcta gtagacataa taacaaaaaa caaacaaaag acgaagactg gaacacaaaa
    18541 ctggataagt tgaagtttat tacatccaca gacgttgatg agctggttgt acatctcagc
    18601 aaccatccac caaagttggt aattgtagat agtatccaaa ctttagtaac gcaggattta
    18661 tcaggatcgg ctggctcaat aggacaagtt cgggaggtga ccgatcgatt gattggtgtt
    18721 gcaaaatcac atcatatacc catgtttcta gttggtcacg taaccaagga tggcaagtta
    18781 gcaggaccta tggttcttga gcaccttgtc gatgcggtat tagagcttaa gggtgaaaaa
    18841 acaagcgagt tgcgtatctt gcgatcacaa aaaaaccgat ttggagcaac tgacgaggtc
    18901 ggtgtttttc gcatgacaga ccaaggatat caggaggtca gtaatccgag tgaatatttc
    18961 ctagaaacaa gagagaagtc ggaacctgga tcggcggtga gctgtgttgt tgaggggact
    19021 cgaccactct tgatagaagt tcaggcgctt gttattccaa catatctgcc tcagccacgc
    19081 aggatcggtc gtggtatttc tgccaatcgc ttgcaaatct tagcggccgt tttggaaaaa
    19141 catgccaaaa ttcctttatc tagatatgat gtttttgtca gtctggccgg gggatacgag
    19201 agtagggagc catccctaga tctagccatc tgcgttgcgt tgattagctc gcatgctaac
    19261 aagccaactc caaagggatc aatttttatt ggagaagtag gtttgctagg agaagtgaga
    19321 atcgcacctc tcttggagag acgtgaaaaa gaagcgatga gattggggta ttcctcaata
    19381 tactcaagaa agttgacaaa aactatcgtt cagttacttg ccaagattgc aactccctcg
    19441 aaaacttagg agatatcccc accaatccac agttcaagtc atggtgttca tttgtgtgta
    19501 taaatatatt ttgacatgac ataaatgaaa gaaataaata cttataagat atatggattc
    19561 taagattttt ataactatag gttgacaaaa aaggggagta gagttatagt ctagatactc
    19621 agttgagcat caaaaacaga agagaatagg tgcaaaaatt ggagaattga agagaagtaa
    19681 aaaaagcatt attatcgctg ggtggaccac gactaacaat gctttttttc gaggagcatg
    19741 tcatgcaagg cgcctgaatc atgctcggta tgcaaaagga taccaagaga actgttagtt
    19801 gtaaacggga tatatatagg acaaagtaga tagcaatgtg tctatgggtg tcttggagag
    19861 gttcggtagt tgggcaggca gccgagcctc tactgggcac ccgtttttaa ataatatcct
    19921 ataatttgca attttagtga aaaaatggta caatatcggc catactaggg cagaatatgc
    19981 cagattcatt actttccacc atacgaattc gtcagtctta tactagagaa cttttacttg
    20041 gcgtgcatct ttcaactgtt tctagtattt caatattaaa aaatcttcgg cgaggtgcca
    20101 cgaatgagag tattagtctc ttgaccaaac tatcaggaaa attagccctt atcattaccg
    20161 tctttgtgat tgttgggcta acgctgtctg caatattctt tttgcctcaa atttactaca
    20221 gaatattccc tgctcaagta gtagtgaccg aaacagctca acagggcact gttttgggag
    20281 gagactttac taaacccacg gccaaatcga ttggtagaca atatgagcca ccagtagatg
    20341 agtcgcttcc agaaggagat tgggtaatca tacctcaaat aggagtgcgc acacaattta
    20401 aagctagctc ggatcccaat gaagccttgg ttgaaggagc ctggctttct ccagagtatg
    20461 gccggccagg cgatacaggg gtaccaatta ttattgccgc ccatcgtttt ggttggcagt
    20521 ggtggtggca atcagattat ggtaaatatc attcctttta ctatttacca gatacactgc
    20581 caggtaccca agttgaaatt atcagcggca agcgcaagtg gttgtatgaa atctatgctg
    20641 gtgaggaaaa caacgagata tctgattatc atgccgattt aattttatat acctgcaaac
    20701 acatcagttc tccaataaga tttgtacgat atgctcgatt aatcgatcca acggcaaatt
    20761 cacaggttgg ggtataatcc ccattaagct attgcggaaa gtcattttta aactgcgctc
    20821 aaatcgaggc gtactaatag aggaagctat tgagagtata tagaaataat cgaggtttag
    20881 cgtgatgttt ctatctgaaa gtttgacttt tcttggatta tctgcaataa tgaccattgt
    20941 ttcgtttcca agtgtcaatt gatttgtcgg tactagcttt tcaaaattgt ctgaaaccaa
    21001 cttttccata tctttgtagt agtaaaaagt agcaatcctt atgtcagatt caccaagacc
    21061 atccttacca actcttactc agagaaaggt acctgctgtt gtgccactta agcgcaaagc
    21121 agctcctgtt attagtcagt caaaccctgt cgattctgtg ccaattgatt cttcgccgaa
    21181 cgaatcttcg ccggttttag ttccagtagt tgaggagaaa gcttcagacg cgacagtaga
    21241 agataaaggt aagccaacag aaccaaaaaa tcatgttcaa ccaacaaaca gcggccctac
    21301 ggatccaatc atttctggtg ttattgatct ttcaagagat ggacatggaa ttgtaagaat
    21361 tggatttgga gatgatgacg gcgacgcata tatctccaat gcgcacatcc gccgacttag
    21421 acttcaatct ggagacatga taacgggacc tgcccggaga ccaaaagacg gcgagcgcta
    21481 ttggggtttg ctccaggttg acagcgttaa tggtatgtcg gtgagagatt ggcaacaagt
    21541 tgatcgagtt aagtttaata aacttacgcc cgtatatcca gactcacaca taaaattaga
    21601 aatcggcgaa gagccacttt cactgaggat tattgatctc gttgctccaa ttggtaaagg
    21661 acagcgcagt ttaatcgttt caccaccaaa agcaggaaaa accaatttgt tgaaggacgt
    21721 tgctgctggt gtggctcaaa accacccaga aattcacgtc atggctgtat tggtgggaga
    21781 acgtccagaa gaagtgactg atattagaag gcatattgcc acaattacca aaggtaaagg
    21841 cgaggtggct gcaagtaact ttgatgagtc tgccaaggat cagtgtcggg tggctgagtt
    21901 ggctttagag cgcgccaaac gcctggtaga aatgggcaga gatgtactaa ttttacttga
    21961 ctctattact cgattggcgc gcgcttataa tttagcgatg ccaacttcgg gcagaacttt
    22021 atctggagga tttgatcctg cagcgctatt tccaccaaaa aaattctttg gtgcagcgcg
    22081 caactttgaa atacagggta gtttaacgat tatcggtaca gctttggttg atacaggatc
    22141 tcgtatggat gatttgattt acgaagaatt caaaggtact ggtaaccaag aagttcactt
    22201 gctcagacga ttggcggaac gacgcatctt cccagctatt gatgtgcaga agtctggcac
    22261 aaggagagat gacttgttga tggatggtat gacatatcaa tcagttttaa cgttgcaacg
    22321 aatgttggat atgattgata aagatgatcg aaccacaaca attattgaac agttgagaaa
    22381 aaccaagagc aacaaagaat ttttagcttc gcttaagacg gggtaacagt cggttacgag
    22441 tgtgtatgca gagaagaaat aaaaatcatg ttcaattacc aaagcctcta acagaaattt
    22501 ttgagtatct tggtttctta agatggtatt ttaaagcacg gggttatcgt tggtttgctc
    22561 ggttcgaacg tatcaaagat agtctagttg atttgttata tcaaaagaga ggtcgataca
    22621 ctcggccatt cttgcactta gcatcgttga gttttgtgtt tattgcttta accatcgggc
    22681 caatgttgtt taatacagtt gaggaaaatg gtcagcaaga agacttgcgt ggtatttcaa
    22741 tcacacaagc gtatggacca aacttttata ctcaacaggc ggaagaagtt cagcagtatc
    22801 gaggtggaga gatcattacc cattatgttc aagaaggtga aacgctggag agtattgctc
    22861 agcgatacag tctgcaagta gaaaccatac tttgggaaaa tgatttatta aagacggctc
    22921 caatcaagcc tggtcaagag ttacgaattt taccaatcga tggggtacgc cacaaagtta
    22981 gcaagggaga aacaatttat tcggttggaa aaaagtatgg tcttgaagga tcccaaattc
    23041 agatgttggt tgactatccg ttcaatgaat ttttaaacga tgaaaccttt gaattggcca
    23101 ctgggcaatt cattatggta cctgaaggca tcaagccaga ggagaaaaaa gtgattccaa
    23161 atcgaagtgc gggctacttg gcacaaacac cgaatgcggg gtccgtggcc gctcaaggat
    23221 cttttgtttg gcctgcgagc ggtatgatta cgcaacggta tagtttttac cacaaggcca
    23281 tagatattgc taatcgatct gcaggatcta ttttggcagc agatagcgga aaggttattg
    23341 gagcaggttg gttggacaat tcaggctatg gtaatcgagt aattattgat cacggtaatg
    23401 gatatgtcac attgtatgct catttgcgaa ctgtccaggt gagtcagggg caaacggtaa
    23461 atcggggaga cgtacttggt caaatgggca gcactggtcg ttcaacaggc acccacctgc
    23521 attttgaaat tcgtcagggt ggtgtgctgc ttaatccgca gaatttcttg aaataatcta
    23581 tgcaagatct aatccaacta ctttgtaagg ctggtaatgg tggtcgagga aaaatatctt
    23641 tccgtaggga aaagtatgtt cccaagggtg gtccagatgg tggagatggt ggcaatggtg
    23701 gtgatgtgct agttgtggcc acccgccata gagcaacgct ggagcattta gccggaaaaa
    23761 ctagcattga ggctaagcac ggcgcagcgg gtggccctcg taaaaaacat ggttttaact
    23821 ctgacgcagt cattattgag gtgccacttg gcaccagaat cacgctttcc agccaaaacc
    23881 agtctgcttt gcatcgccag ttaatttgtg gagatttgcc aattaagcgc ggggatgttc
    23941 gatttgttca atacgaagta gattttcatg gtactcaaat taccgacgtc gattggtcag
    24001 aggcagaaaa actcatacca gtagatcaaa ttgatgtgat ggaacctcca attactggtc
    24061 aaactattgc tgatttagtc gaagatggac aatctatatt attatgccag gggggctttg
    24121 gtggtaaagg caatactcac ttcaaatctt cggtagtcac cactcctaga aaagctgaat
    24181 atggtacgcc aggtgaggct cgcactgttt ggtttgagct tagagtgctt gccgatattg
    24241 ggttgatagg catgccgagc gttggcaaaa gtaccttgtt gtctgtacta acacgtgcca
    24301 cgccaaaaat tgccgcctat ccgtttacca ccttggaacc acagcttggt cggttagttt
    24361 tgggagaaaa agaggtggtg gtggcggata tacctgggtt aattgaagga gctagtcatg
    24421 gcaaaggctt gggcaccgac tttttgcgtc acattgctca ctgccaagcc ttgtgttatg
    24481 tcgtggcagt accagaagaa gtgttgttgg ctcaggaatc aaccgaaaaa gatattgctc
    24541 aattgctttg gcaacaatat caattggtca aacaagaagt cgtcaccgct catccagaat
    24601 tagcctcaaa gcaatcgttg gtagtcctca ataagtgcga tatttattca gaggttatga
    24661 taaaaactat taagcaagca ttcgccaaaa acaaaacccc gctaacagct gtcagcgggg
    24721 cgactcactt tggactatct gaactcaaaa ctcaactaag ctagtggttt taaggattat
    24781 ccgtaggagc tacttcctca gcaggacttg gggcgactgt cggtgccaga gttggtgcta
    24841 cttgatcgtc tgtagtgttt tgtaccgctt gctcagcagg agtattgagt tgttgatcga
    24901 gtaatgaggg ggtcgaggca ttacttgttt cggtaggctg attggtgttg ttattcgttg
    24961 attcctcagc gggacttgtt tcctcaatta ctttttttaa ttcctctaac tctttggttg
    25021 ctttaacaaa atcctcagta gccgggtcaa gcagggtgag tactgcctca tattcttgtc
    25081 tagctgcgtc atacatttcc aaggccaagt acgcttgcgc cacgttgtag tgggagttgg
    25141 cgtagtctgg ttttacgttt actgcctgtt gaaacacact taatgcttgt tcgttttgtt
    25201 tggcatttaa cagaactcca cccagagcca atcgtagctg tggatcactt ggcgatgttt
    25261 gaatggcact cacataggtc tgcgtcgccc attgctcggc gtcctcagca ataccaatca
    25321 tgttttgata cacctgagct agaacttgcc agttagtggc gtcgttggga tccaagtagg
    25381 ctgctgctct tgcctcagta accgcctgtt gtaacagtgt gctgatttgt tgtttttgtt
    25441 cgtcagtaac ttcttcgcta tttgacaagc cagaggctat gagcaaatta gtgatggcat
    25501 aagttcggcg aacagagtcg aggtatggta atgcgcgtac tgctgcttgc tggttgttgt
    25561 ataaccccag cgcatcattc tcgagtgagg cttgagtggc ttgatgtgaa agcacaagtg
    25621 catagtaggc tctaccaact ccataaaagg agatgagtag cacaacagca aaaactatgg
    25681 ctaatgcata gactgggagt gccggagctt tttttgtact cgccatacca tcgacgccaa
    25741 agttcatggg gttcagtact accttgtcaa ttagggcggt gaggtgaaac ttggggcgac
    25801 tatgtgatcc agccaacaac gccaccacca agagtgcctg tagtccaatg agaactggac
    25861 tggctggtag gagtatgaac cagaacaccg tcgtgagaat tgccaagctg agaggtaact
    25921 ggttttttga ctctgctcga gcttctctga caatttggta tgttacgagt gcccaagcca
    25981 gaaagccaat aatacccaca gtagagagga gggtaatagg tgtatttaca gctgaggtga
    26041 aaatagctga ccaattttca gttccattca caaacactgg tttgtagatc ctgtaattat
    26101 tgatgtaaga actaggaccc gaaccaatca ttgctgtgcg tggcgagcgt atgctatcaa
    26161 gcataattga ccaactggca gaaaaagagg gaagcacgat tggcgccggt ccattaggac
    26221 gaactgccca aagatgcagc acaattccca caccaacgat tgccagtaaa gcaatattaa
    26281 tgggagagcg aagacgttcg ctaataatct tgcctactaa agctgtcgca gctatgacta
    26341 agatctgaat ggcagtcagt gttgagctgg ttaagctgaa ggccaagctc gagggaatag
    26401 ttagtccaaa tgcgcgattg agcaactggg caggaccaaa acctactaat tgaaggatcg
    26461 ctaacagggt gattgccact ccagttccag ctaatgcagg aaccacatat ttaccagcgt
    26521 tttttggcaa caaagcaccg ccaatgagga tgattatcat ccaggctatc cagctgccgc
    26581 ccacacccat aagttcttcg actggatagg gtttagctac gaccgcagaa actgctgtaa
    26641 caatgccgaa aattgcagtt ggcagtaata ctggagaaga aaccatttgg aagcctttgt
    26701 gaatgagggc gcgacccaca taaataagac ccaaaataat tgcgccaaac agcagcaaga
    26761 atgattttgt gtctgtcaca aatccgtccg ttatgggtac cacgagtaac ccaaaaaaga
    26821 aaataagtga catggtgatt cctagagcaa aagtggctag ataatctttg gtattggtat
    26881 ttagcataga cctccattaa atcagttact acagaggaac acaaggcgaa gttttacttt
    26941 gtatatacgt cttttttttg gcattaattt actatccacc aggtaaagct aatatcggtc
    27001 gcggtggtgt tgtccaggct aacggtgaag gagcgttcgt cagttgcttg tgcgattgat
    27061 tcatcagctg gagcattgat ggttttaaca aacagcactt ggttattagt actacctaat
    27121 ggtgtcaaat agatcagtga gtcgcgacgt acataagggt tgcggatgat taattctttg
    27181 ttgccggcgg gcaagatagc tcttccagtg gtttgagttg cttcttgagt gagctgactg
    27241 gattcggtag cggcgccggc cacatttggt ctctccaaga ggtcgcgacc caaactgagt
    27301 gaagagaagc tcgcgttacc ctgcgccgag acggtagcta ctggagtacc cgcgtcgcct
    27361 aagacttcaa actgaccact ggggctggca atttcacccg agatagaagc ctctgagcca
    27421 gcagttggcg caccaagatt caacttgatg gattcccctg ggttgagcgg ctcgatgacg
    27481 ttggctagga gcgagccttc cacactcaca ttgccagcca catagagatt gccgttgatg
    27541 gtaacctgac cagcttcgtc caagatcatc aaatcaccca tcaaagctag catgcctttg
    27601 ccagatggtt gaatgaagaa aaccggagct tcatctggag tactagcgcc agctgagaaa
    27661 ctcaggtagg tatcagttat ttctaattta ctaccaactc gcaaggtatt gccaactccc
    27721 aggctgtcac caatatacgc aataccgttc accttgaagt agcgatcaat aaaggcagcg
    27781 ttggcattga gcacaatgtc gtcctcaact aaattaaggt tagccaacga ttgatctaat
    27841 tgagcagcct ggctggccgt actactgata ctgcctaata cttggtacaa ggaggagtaa
    27901 tcgtcctgag gcgaggggat gttaccagtg atcacatcca tgattgatgg ttgctcgagt
    27961 agagcagcga tctgacggtc aaggtctgca atggtctggg catatagcgt gccggatatg
    28021 gttgcttcgg caaactcggc ggtagtggct ctgactgagt caagctgtgc catgcctgcc
    28081 tcaagcgcat cgatgcgggc tgagttggca tccagctcgt tgactgtcat gacgcctgct
    28141 acactcgcat tttgactgac taacaatgag ccaagctggg tttgaccatt cacatttaga
    28201 ctctccgtag tcaccgaggt ggcatcaatg tcttgagtgg tgagcagatc agtcacagtg
    28261 agatcttcca caattggttc accggtggtg gattctctct ggaacacctc cgccaaccag
    28321 gcagattctg aggctgttgc tactggtgca ggagtggggg agacttccac aatcacaggg
    28381 ttagttgctt gattttcttg aataattgaa acgacatatt cacgtaacgt ttgaccattg
    28441 atcgaaaggt tagcaaccga caaatcagcg atctgggctc gaccagtgac caccagctct
    28501 ttggtgcgag tcaaaccggt ttcaataatg gcagtgatag ttctggtaaa ggcagcaaag
    28561 cgatcgttga ttggtgtggc agtggtagaa tctacgactt gataggtagc attgtcagct
    28621 tcaaagactt ccaaagcgcc aaactgatcg attgataggt ctgggtaatc agccaacata
    28681 gcgttgactt gattgagaga gttttctagt tgagtatttt tttgattgag cgcttgtact
    28741 ccgagtatgg cataacccag ggtgttgacg gggttaacga tggtgccatc ataatgcatg
    28801 gcccaaggtg cttcatctgc catgatgccc acatactcag tttccgaatc gctcgtaccc
    28861 atacctggtt tgtagttgaa gcggtagact tgagcattca ccagcgtatc aagtgctacc
    28921 gaagcatcca tcgttcctag aatatttttt gtagtacgca atgagtttgc agcggtccaa
    28981 ctggttactc ctgatccgtt ggtagttaat tgctgaccat tgctaccagt accggtgggt
    29041 agcgtcatgg tccaactccc cgctgcagct gaaggttgga tttgggtgta accagaggta
    29101 ttgccagtta atcttagtgt gcccaatgtg ctggctgcag tacccaattg taacattgct
    29161 ccaggggcag tggtgccgat acccatgttg ccactggcaa caattagacc ataattgcta
    29221 gctgcacctg aggcactgaa gtacccagcc acattggtat ctccaccttc gccagttcga
    29281 gtgttggtta catacgcacc gtaggtagtt tctgttgtac cattagttcc agagttggaa
    29341 atatttagca ccttttggct actggaagcg gcaccggagc cagtgacaac caaattcatc
    29401 aaactaccag aagtaaggga tgagctactc gcatagattc ccgaaccaga agttagtgag
    29461 ttggcagtaa tactcatacc tctacctgtt gttactccgc ttgagtagtc aaatcctgag
    29521 tcggttccag ttgtggctcc actattcaca aactctattc ctttgtttcc ggtgttagtg
    29581 gaaaaactaa aagatccact gccacttgat tttgaaaagc ttgtggtgga agaaagctcc
    29641 aatccagatc cagccatacc aacataatca gaaaaagtga aacccgaact tataccaaca
    29701 gcatcaaaag ttacgccacc agctgatgag acggttgttg agtagtaatt tgaagcatca
    29761 tagccgaggc ggagttgttc agtagttttt atgccgtgaa ttagagccga tgggctcgtt
    29821 gttcctatgc cgacattgcc
//
LOCUS       DAOQZP010000003        29218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_1870_length_29218_cov_5.488119, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000003 DAOQZP010000000
VERSION     DAOQZP010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 29218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29218
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1870_length_29218_cov_5.488119"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..234
                     /locus_tag="PKH60_00280"
     CDS             55..234
                     /locus_tag="PKH60_00280"
                     /inference="COORDINATES: protein motif:HMM:NF039196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16519.1"
                     /translation="MSKGDTPPKITDSYRGDFNEIKNFRVCPNLCCDARAPARATHQH
                     TLRKVMIHKHGLLPG"
     gene            complement(1042..1488)
                     /gene="nrdR"
                     /locus_tag="PKH60_00285"
     CDS             complement(1042..1488)
                     /gene="nrdR"
                     /locus_tag="PKH60_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNQ16520.1"
                     /translation="MKCPYCATTETNVIDSRVVEEQTAIRRRRVCAKCDKRFTSYERV
                     EGIELKVVKKDGTKEDFTREKLKRGIVKATWKRPVSMSDIDELIEDVERRLRRKDSKE
                     VKSWEIGNMVLNRLKKLDPLAYLLFASVYRDFNDIEDFKQEISKIV"
     gene            complement(1656..2351)
                     /locus_tag="PKH60_00290"
     CDS             complement(1656..2351)
                     /locus_tag="PKH60_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16521.1"
                     /translation="MPSNQPNNSNLATTPEKLLNEHVLFFPLLLVAVIAWYAYRWLFS
                     FPVWFDETLGKAFFFGLPVWFYTVVSKRKDVLEPLRFSIFQQGLLLGVALGGLYGFIT
                     STMSLLQSGGVVESAQLFQSTQFWYEFALALFTGFWETLFFYCFVAMVVFDKYKKVSL
                     VWQMALIVIIFLVFHLPNAFLRFSPALVMSQVLLLFLFSLGQALVFYRWRNAYALMLS
                     HAIWGLVLLFHGT"
     gene            complement(2329..2940)
                     /locus_tag="PKH60_00295"
     CDS             complement(2329..2940)
                     /locus_tag="PKH60_00295"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ16522.1"
                     /translation="MYQNITISGLPGAGSTTLLQHLKLVLKDEGWQGFSGGEFMRAYA
                     TEKGYFDPSSGGHHDATVYSDEFDREVDMDMRSKLMSEKKWVLESWLSGFLAQQVPGV
                     LKVLMVCSNKAVRIDRIVNRDAISPDLALANMNNRYEKNLKKWQRVYAQEWQDWVVAP
                     NRVKSSDPIDFWRQDLYDVVIDTYSVSQLEATNIVLNAIKSTQ"
     gene            complement(2947..3441)
                     /locus_tag="PKH60_00300"
     CDS             complement(2947..3441)
                     /locus_tag="PKH60_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16523.1"
                     /translation="MHPDIASSTWFTYLDLDLQRMVMLSLRLYERQELSLEQYDDYTF
                     VVFPMSKAYEGFLKHFLLNTQLISQETFNDRKFRIGRALNPDVSPRHRDEHWLYEDVV
                     RYCSAELARELWETWLRCRNRIFHYFPKDSQVLTLPQAGEYLKRMQDTMHEAVTCQIS
                     LEKE"
     gene            3521..5917
                     /locus_tag="PKH60_00305"
     CDS             3521..5917
                     /locus_tag="PKH60_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNQ16524.1"
                     /translation="MQSLRLLEKKYFKQFKRFKKKNKGNLFVSLVRFYLLGGSPAVRS
                     SLKKTIKTLPHKQRPLTRLLKLPTRIIKQRWFQAVVLLGIATMIGGFWFLLQDLPSPR
                     NLTSQENYPVSTQIFDRNGVKLYEIFAGENRIPIKLESLPKNVINATIAIEDQHFYRH
                     FGFDISGIFRAVITNLRGNRLEGGSTITQQLVKNALLNRDRTLQRKIKEVLLSVMTET
                     LYTKDQILEMYLNYISYGGTAVGIEAASQQYFGKPASQLSLAEAAILAGLPQAPSRYS
                     PFQSDQSAAKNRQSEVLRRMAEDDYISEAEAEIARTDTLNFALSKTDILAPHFVFFVR
                     DQLVEKYGVETVEKGGLRVTTTLDYDLQQTAQASLSAELDQLVKYQVSNGAALVVTPN
                     TGEILAMIGSKDYFDPNADGKVNVTLAERQPGSSIKPIMYATAFQNRVLNPGTLLVDV
                     KTCFTVPGQKDYCPKNYDGSFRGPVTVRQALGNSLNIPAVKALNAIGIETFISQATKM
                     GITSWTDPSRYGLSLTLGGGEIRMIDLAQAFAVLANEGVKVPFSPILKVEDYRGNVLF
                     ELDPAARKEALDHLNIFEEETEKNDLQRVMNRAPAYLASHIMQDNTARQWAFGSNSQL
                     VIKNQVVSAKTGTTNNLKDNWTVGFTPEFLVITWVGNNSGKPMNPYLVSGITGAAPIW
                     NDIMSYVLKNREPMWQEKPSDIASAKICATGFPPTAGDGCVPRYTELYWQKSQPSEVK
                     LVKQQVWVDPATGQPPPPGIFEGELKLEEHIYYQDPGSPLQCADCVPVDERSQLELPL
                     "
     gene            5914..7167
                     /locus_tag="PKH60_00310"
     CDS             5914..7167
                     /locus_tag="PKH60_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HNQ16525.1"
                     /translation="MSYQIQKQFLKVQKPLVYAKKKWRGLKIILFLCVTIFMGVSIFL
                     WQTWQPPLTAPISSTTTFSFLPYDSVMGSITQPKLIYGFLPYWNLEKTTLNNSLTHVA
                     YFALTIGGDGTLRTQGDEESLTGYRKLQSESFLDIIEKTQKDAIQLELVLKQFSPQET
                     TAFLLSKQAQAKFYDQLDSVMLAYPFRGINLDIELSDGQQLQSKLTEFVTDLNRHLDS
                     VAPNTTVSIDVYPSAAQGGNIWNLSELAPHVDYVIVMAYDFHRRQSAQAGPVSPLLGG
                     DVYQNINQHLKEILELVPAKKILLGVPFYGYEWQTTSRDEQALTYPDSGSTASYQRVL
                     DILAQKEELKVQERWNEIALSPYLSYEKSGQTFVIYYENTRSLSYKLDYVNQLNLAGI
                     AVWALGYEGDANELWSVINDKLALP"
     gene            7215..9452
                     /locus_tag="PKH60_00315"
     CDS             7215..9452
                     /locus_tag="PKH60_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNQ16526.1"
                     /translation="MDYHKLSQSEYQRVRRVLALHLYQKRLALRGRYRTFSVNNASDG
                     IIFSSQSLLSPTKRLFLSIQQCNQILINTILFFTRPFVFPLRYYPLHTIILLTIFGII
                     WGLFYKVNIAIFVDLPDPNLLSEQLPYVSSKITDRHGSVLYRLYDEENRTLIPLREIS
                     NSAIEATIAIEDKDFWHHHGFSLRAIIRAALSNSQSELTQGGSTITQQLVKNRLLSPE
                     RTIQRKIKELLLSVMTEQRYSKETILTMYLNQVPYGGSTYGIEEAAQRYFGKSASKLD
                     LAESALLAGLPAAPTAYTPYGNSPERAFARQREVLRNMVEAQYITQDEAATAAQQQLA
                     FRQDSIDIIAPHFVMYVKNELAKTYGENILNRGGLTIKTTLDIPTQMISEKVIEEELT
                     KLAKLKVSNGAALVTNPQTGEILAMVGSKNYFDFANDGQVNVTLRPRQPGSSIKPITY
                     TLAFESGFTPASTIVDAPISFTILGSEPYSPKNYDGTYHGPVTLRQALASSYNVPAVK
                     LLSVLGVNNLISLAEQMGITTWQDRSRFGLSLTLGGGEVTMLDMVTAYGTLANNGQRV
                     EINPILEVTDLDGKILYNNICAQNPTTCHFPQVIPSGIAYQVTHILKDPVARIPAFGP
                     FSVLTIPGQEVAVKTGTTNNLRDNWTIGYTSERVVATWVGNNDNTSMSYVASGITGAS
                     PIWNKIIRTQLSAEKPHVFQRPDSIISMKSCFTNQEEVIVVGQPLPPPCNKQLSEQID
                     TLIEP"
     gene            complement(9439..9663)
                     /locus_tag="PKH60_00320"
     CDS             complement(9439..9663)
                     /locus_tag="PKH60_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16527.1"
                     /translation="MFTSTETSLEKKAGDLEELSRIREAEKESKLREARLKSQAKAAA
                     YRRMLLSKIIAPVILLLTVIIGSLLWITAR"
     gene            complement(9667..10380)
                     /gene="cmk"
                     /locus_tag="PKH60_00325"
     CDS             complement(9667..10380)
                     /gene="cmk"
                     /locus_tag="PKH60_00325"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNQ16528.1"
                     /translation="MAAQSFQIAIDGPVAAGKGTISRLVADRLGFLYVDTGAMYRVTG
                     MLADRAGVSIDDENALVELVEKVTIQMRNPSETEKDGRLTTVLVDDTDESWSIRTEKI
                     GDLASKVATYQKVRAVLVAKQQEIAQSQNVVMEGRDITYRVLPDAQLKIYLTANEIVR
                     AKRRQFQLQTRGMLAEFEEVYQELIERDKRDSQRTTDPLTILPEAWVIDSSDLSIDQV
                     VDIIVGKAKMMYHRFQQTG"
     gene            complement(10400..10618)
                     /locus_tag="PKH60_00330"
     CDS             complement(10400..10618)
                     /locus_tag="PKH60_00330"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HNQ16529.1"
                     /translation="MSKSYFWPAAMVVMGLIFLADQVGMLPKDFWNLWPLILIIVGLG
                     GLLTADRSEWLSDTTTKKSKPAKKSKKK"
     assembly_gap    10729..10828
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11012..12925)
                     /locus_tag="PKH60_00335"
     CDS             complement(11012..12925)
                     /locus_tag="PKH60_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16530.1"
                     /translation="MAIVHSKFSLGLPNPEGESRFWEKSTNQNYSCLIFSVVGKTARE
                     TSASIEKFIQSTTMTDAQSLFEVVSTIETRCQENSVDVSVVGVIIENQKLYVASLNGG
                     VFLVRGSKRGALLTSQPIVQVLQGVFKQADVLVIYTNSVQKQIQLLFSKGFALDQIQT
                     LAQEFQRAIRKMNHSETMAAAFIASPKESENESNELPTVAVKEKQNQLLNIFKSGLTF
                     GLEAIKSSINFLLWFVGSILRKSKKSEQNVQRTSLVKLLLHPAKTYVGVHIPRKQLVA
                     LIIFLIATLLVTITVVVTHYKTLEVKEQLAEKLIPYQQTLSDLEKQAKTAPAQAYLGV
                     QQLIQNLSTLESEYLEPQSAHKQVLLTLQQAQNLAQQLYESQAIEQLPIFDDLRKISP
                     TFITSSSSSNNGNLIALDAQQQYVISYSLADKIGKEVVLDSTNQLRVVTSTEKMFLAL
                     GGGVFALSTLDSQLGINELLETSDAVTNSQLIGGYENFVYLLSSESRAIFRYEIKSGK
                     LVNRATWIGSSSGVPFGDSVSMVVDGDIWLGTNTGQITRMRSGRTVEFTVAGLSEPFE
                     STLLLATSLESPNLYVLEPKKSRVVVLNKDTGEVVNQIQNRTLGSANTIVYDESQQLV
                     LVVSGSILYRMPL"
     gene            complement(12935..13183)
                     /locus_tag="PKH60_00340"
     CDS             complement(12935..13183)
                     /locus_tag="PKH60_00340"
                     /inference="COORDINATES: protein motif:HMM:NF040135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16531.1"
                     /translation="MNPTIAPIFDGILQDPNQLLLGFVKMLFVVGGMLYALFALLVIR
                     QIQLMRTTVHTGFSAALMLVGISHFLLSVVVVIYYLAL"
     gene            complement(13180..13923)
                     /locus_tag="PKH60_00345"
     CDS             complement(13180..13923)
                     /locus_tag="PKH60_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16532.1"
                     /translation="MPDIFSSSSTPPERVQIHRKHRLAHRVDQEGHLLDTNGVISSTH
                     KSRDVSEYSHLMRDMETTHSPFASFVAKPYKLKFSTQAPNEVVILLLRQHPVTQVWWI
                     ILALLGVGFPFLLQKIDFFNFLPANYQMAAMVGWFLVLLGFIVEAFLKWYFNVYIITD
                     ERIIDVDFNSLLFRNISSAKIDNIEDTTAMNAGFLSAIFDFGTVVIQTAAEKREFEFA
                     GIPHPSKVTTLINDLILEEEREKLEGRVS"
     gene            complement(13954..15735)
                     /gene="lepA"
                     /locus_tag="PKH60_00350"
     CDS             complement(13954..15735)
                     /gene="lepA"
                     /locus_tag="PKH60_00350"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNQ16533.1"
                     /translation="MRIRNFSIIAHIDHGKTTLTDCFLRLTNAITANEVRVMDSNPIE
                     RERGITIKLAPVCLRYFDPQSQQEYILNLIDTPGHVDFSYEVSRSLAACEGVVLVVDA
                     SQGLQAQTLSHYQLAHSLGLTIIPVVNKIDLPSADVDKTVLEVMELTGLPEDQVICVS
                     AKSGINTEAVLQAIVQRVPPPKVFPATEGKALLISSHYDQHRGAVALIRVFEGSFTAG
                     CTVRLLGSQTVFHPSEVGVFVPKMQPQAALNAGEVGYCVTSLKDVRGLLVGDTLTTSP
                     VKEKVAPLVGYQPPTPMVYLEIYPVESDEFELLKESLEKLVLRDAALQHTPAHSNALG
                     SGFRVGFLGILHAEIVLERLEREYDLNLIATAPSVHYKVQTTDGAWLMVTAPSQFPDP
                     SQIKQVLEPIAVVSLITPVAYQHECLELVRRNRGTFISAQPIAGQQQLTIKMPLAELI
                     TDFHDRLKSATSGFASLDYSVGEFSPADVVLVSIMLNKDPIEVLSFLTVRPQSESRAR
                     KMVEKLKDILPKQMFEVAIQAAIGGKIIARETLKAYRKDVTAKLYGGDVTRRKKLLAK
                     QAKGKKRMKQFGSISINQQTFLQLLKQ"
     gene            complement(15748..15987)
                     /gene="rpsT"
                     /locus_tag="PKH60_00355"
     CDS             complement(15748..15987)
                     /gene="rpsT"
                     /locus_tag="PKH60_00355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNQ16534.1"
                     /translation="MPILKNAQKALRVSQRKAAYNAVVRSRMRSVLKQLKTTPTKELL
                     DLSYSRIDRALKRNLLHINKAARLKAQASKLLKAA"
     gene            16010..17704
                     /gene="murJ"
                     /locus_tag="PKH60_00360"
     CDS             16010..17704
                     /gene="murJ"
                     /locus_tag="PKH60_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNQ16535.1"
                     /translation="MFGRFKLPTLLSNNTKWLERQQTTILSAALIIGIANIISSIAGL
                     IRERLLISYFFDTSESRLNLEAFQVAFQIPDALFQLLVLGAVSASFIPIFTHLKKRDS
                     KTAFASASIIMNVVLLVFVLVSAVIFVFAEPLTLFRTGSGFTPEQLAVAVQLTKIMLF
                     AQFFFAISNFLSGILQSYQRFIVPALAPILYNFGIILGVALFSQNFGIYAAGIGVIIG
                     ALLHMAIQLPFAYLLGFRFTLSFNLSSPGVKKLFSLMPPRFITYAINELQQLSLGFFA
                     TSLGNLSFFVIRLALRLMTIPIRIFGVPIGQASLSFLSEESTDEDYNRFRSLLTQSLN
                     HVAFLAFPASMLLLILRIPIVRLVFGASNLPWETTLATGRVVAIISLSVVAQALVQLL
                     IRGFHALNDTKTPLYITLVTAITYLIGCWFFVFYTQIGLLGIALVILITAFLEMFLCL
                     FLLDRKIGQILTKSFLFPQAKILLASFFMAVFLYLPFRILDELIFNTSKTVELIGLTI
                     TTSSIGLLVYLYFSALLNVRELQLVGSTFSSLDRWRKTLSRTTEVLSEPTNEEGLR"
     gene            17768..18322
                     /locus_tag="PKH60_00365"
     CDS             17768..18322
                     /locus_tag="PKH60_00365"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNQ16536.1"
                     /translation="MPKRATTKISINLLPEDDFLLGSFGKVIRWAVSVGRYLVIFTEV
                     IVIISFATRFSLDRKVTDLNQEINKKVAIIESYAGLESEFRSAQEKLSQYSELEKQRN
                     LTDVFEQISNVTPPDVVVTQLNVKKDGISITGSTLSQTAFNHLINNLQLSPFFSDIRI
                     DSVENTQDSSGYEFSLQAKSKNAN"
     gene            18312..18911
                     /gene="pilO"
                     /locus_tag="PKH60_00370"
     CDS             18312..18911
                     /gene="pilO"
                     /locus_tag="PKH60_00370"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNQ16537.1"
                     /translation="MPTRQQTSLSISNFYRHPIAAVSTELLFTIVFILVLAIVAIQPT
                     LKTMADLSKEIEDKTKLNKSLQDKVAALNTAQAEFYRWQAQLALLDTAIPDNQHTILD
                     VKLFEKLASEKNVVIGKIGLSEYPDATKPLSQKPIVNDLPISITIQGDYISIKSFVSA
                     LLNSRRVFVINSINFSVSTSRGGQQDLTATLSMNAPYYQ"
     gene            18925..19239
                     /locus_tag="PKH60_00375"
     CDS             18925..19239
                     /locus_tag="PKH60_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16538.1"
                     /translation="MKMPLLDIFAERTKGFFLILLSLLFVCVIFWIIISLFSSQKTTK
                     VPESSQKLATPLNPVLDTSKLDRLEGKTYYTPEELQSFPINIIETDAKSRTKRVIQIQ
                     PT"
     gene            19304..20854
                     /locus_tag="PKH60_00380"
     CDS             19304..20854
                     /locus_tag="PKH60_00380"
                     /inference="COORDINATES: protein motif:HMM:NF027967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNQ16539.1"
                     /translation="MLTSKMNGAVLAKRIPTLLGLAVLVVGLIVGTIVFGQGTGVFSP
                     RATPETTPKQVKVTNVSDRAFSVSFITEESTTAFIKYGTDPNKLSLQAGDDRDQLYGT
                     IKDYNLHHISATSLEDRTTYYFVIGTDQGALYDDNGEPFSILTAASGGTPPDAKTIFG
                     SVTTAQGGPAAGSIMYAKLEGAGELSQLVQDSGSWAIVLPTARTEDGSSYATITTSTP
                     LVLLAQGFPQALTAALNTTVGQFEMGYTFTLGQSGGGASSPTATPTAAQVSPSPTVTP
                     NLSGSPTPTPVDTGSGGGGLGELLGESPLPTPTPTEAIKVDLTAQGEQIVTTEQPIIV
                     GNAPAGVVVNLKVNSDTQIEQQVTTNSQGQFSLDLEALGAQLEPGTHSVEYSYTDPTT
                     GRLVTETKTFTVSSGGVQLAQANITPSPTRSLSPTASPTPISYGTDYPYGTSPTPTTS
                     YATGSGQQASVSATPTQPVSTRSGMPSTQSGVPVSGSVGTTLVLLFGGAFFLISGLWS
                     FWIAKEIE"
     gene            20885..21409
                     /locus_tag="PKH60_00385"
     CDS             20885..21409
                     /locus_tag="PKH60_00385"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNQ16540.1"
                     /translation="MKKKNDMILVPKYSSSFQKRVFDLLVGTSLLLCAMPAIVLTGTA
                     IVIASGWPVFYSQPRLGKNKQVFTLYKFRTMKIGADSRQKQFSKLNIAPAPMFKLKKD
                     PRFTSIGYYLSHTGLDELPQIFNVLKNDMSLVGPRPLPIAEGKALKDDWNWRWQVKPG
                     LFSYWVLSPHRYQS"
     gene            complement(21517..23037)
                     /locus_tag="PKH60_00390"
     CDS             complement(21517..23037)
                     /locus_tag="PKH60_00390"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNQ16541.1"
                     /translation="MKARITTYSLISFLIGLIIMGYELTVSRLIAPFYGNSVYTWGIL
                     LSVVMAALSLGYFLGGRLADKTKQPTTTLALTLFLSGFILFLSYFSSQYLLQQSLQFV
                     AQSSSAWLYLLILTIAMLCSFAPAMTLLGMVSPILLKLSVKGLKSIGNTAGILSMVGS
                     FGGITGSVMASFILIPTIGVLHSIQLFSILLSLSSLLVVFNRKTSQLVALLIIGIVLV
                     DRIYQVFVIKVQADVVMESLYNHIKISDHNGRLYLSTGNARGVQTISIPESGIMNNYL
                     DYLALAPSLLQKEKPHSVLLIGVAGGTVIKQLNQFFPDAVEIDAVEIDPTMIEVAKDY
                     FALSDSEANIIIEEGRKYIATTQKKYDVVLIDAFSTDLYAPSHLMTQEFFAQIKTVLQ
                     PDGIMVFNAVSPQLAQDGPTLFQVIVNTVASIFPYTYHAPVRDNQFLISNNMIYASNS
                     QIGDVENILDFTDPAVNEVATTIRERIHQVNIDDELPFLTDDKAPVELLYWKMLGK"
     gene            complement(23102..25567)
                     /locus_tag="PKH60_00395"
     CDS             complement(23102..25567)
                     /locus_tag="PKH60_00395"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNQ16542.1"
                     /translation="MHKLVSKNAHLFILVATSFIAIFTTHPAKWFLVVIALIVLRQNI
                     LGVLKKNFFIFFSWILYLIYCGASLLWTISVPISLSGLLDQIGYFLYFVYVLIICEKF
                     GRMKVFVAMSTYIVVLISINIVLLLLPVSNQLGGSTDFLLPAYGHNHLSSVVLLVLPA
                     AFYLINKKLDRLGAMLIIFSLISLISSSGRILLFLGLIEIIVSFILLIRTNHLTNKIK
                     ILFLSSIIVISATIFWFLIFSTKVNVCYGSTSWWCKPLEIGARKSYLSVAVKNLIDEP
                     LLGTGLNTYSLIARKYTEQPGVYSHYAHNYLLQIITELGTFGFLVFTLLFTTILTSIR
                     NSKNSNRLEQFLVIGLLFNFFNALLDYDWNTSTSFLLALMVLGIVISKSSANTNKAGK
                     FLFISNISSLLVVIRLLVFVLSFFILIYITQAVLIAINKPDYACLVFAGNKQAYELLL
                     SGKNNKISTDCVKKVDNYFINDLYYWQYKNDNAESESDRINAHYNLIRLSPWHSLAGS
                     TLDFYIENNQRESAEKYINLVIENTRRLKKTNSLKHWQLYKLTPEIVRFGDYLFSQKE
                     YGEAGAYHRIAYELDPWALSFQTPIIANRVAMDGDEYTYYEHLRTISPEYFRSQAGYY
                     SDYYLSLAIMLSGTNSESTQLIEITKSASQLTDTINLALWYRLLWTIEGDPHNPLSNY
                     DNHNQRSWVFMISDEMTQILKRNSNISFFNELDREESMSIAKYLTNLGRISLSDNQID
                     LAYNLSWSATKFIDEKSPAFSQTGLLCENVMNLTCAEKEYRNCLAVSQQLNQECSEGL
                     ARIVNNIPDRNRYWSITEKLLLY"
     gene            complement(25677..26405)
                     /locus_tag="PKH60_00400"
     CDS             complement(25677..26405)
                     /locus_tag="PKH60_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ16543.1"
                     /translation="MRKNRASVVFSNVTKSYFFRHEKPTLSDFVYFHNRKNESFTALS
                     SVSFEVKQGERLAFIGVNGSGKTTVLKLMAGVTSPTSGNIHLSGRAVSLIDLAAGFHP
                     DLSGVDNIIINALLIGMKKTEVKKQLDNIISFADIGQFIDQPFFTYSEGMKLRLGFSV
                     GVHSNPDIFIIDENIAAGDENFKKKATTKIHELFNSGKTIILASHMLDFIKDNFNRVI
                     WLEKGKIRMNGGVEVVDSFIASFK"
     gene            complement(26408..27130)
                     /locus_tag="PKH60_00405"
     CDS             complement(26408..27130)
                     /locus_tag="PKH60_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ16544.1"
                     /translation="MTKRELVVRYKRTELGFIWIVVTPIFQMLVIGLIFQHLLSNSIQ
                     NYFLYLLSGLLAWNYFSQTVLKNTQAIVNERHMIKKSRFPREIIVISISLAHLVHFIV
                     GLTLLLVVSIVNGVQTNIFHLVLGIILLFVTTIGLSLLTSAINVRFRDTSFGVSILLQ
                     LLFYSTPIIYSNNQLPVNIKSIVDANPLSYIVSFFQLALSGNSQNISTFNATIAIVVS
                     LTLLLLGTLVFKMKSRSFDDWL"
     gene            27393..28208
                     /locus_tag="PKH60_00410"
     CDS             27393..28208
                     /locus_tag="PKH60_00410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNQ16545.1"
                     /translation="MTNKRSLSLDVIIVTKQRYSQLLECATRICNNTQKPANLIIVDS
                     SSKRDEKILLTISKLCKLKKIKLTMIEASDHGIAYSRNLGIRSVKSTHFAFVDDDEYV
                     TKYWCRKILIFFENNQDYQVLCGPKIPTNLDNYLNQTWASIFLPSLSYSGVANFVPSG
                     NSCYQTRFIRENQLFYDERFSSSAEAHAMYSNLIHHGAQCFFLANLCILHDWNTSLTT
                     FTKRWFEYGRGIHFFFHEYLASEFSGSLQRYLFLAPDHLFRFFPFRNNRLVAQ"
     gene            28255..29166
                     /locus_tag="PKH60_00415"
     CDS             28255..29166
                     /locus_tag="PKH60_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HNQ16546.1"
                     /translation="MNLTYFLKHPELYPAAIGISQTQFEQLVPDFEKAFTKCWYRKAW
                     SYQRQRGVGGGRKPLLKHTRQRLFFILFYYKTYPTFRLASILFGVDKETIHRWKVFLE
                     QVLWMAIGGQLILPKKKIKTLTGFMEIFPDLNEFIVDATERRVLRSKDQSTQEFYYSG
                     KKKCHTIKNQVIIHPRTKRILAVSKTVEGKRHDKQLCEDDGLILRAPPGAVGSGDLGY
                     IGVCELNPLIKVALPLKKPRSRELNPEEKETNRVISSVRVRVEHVIGALKFFDILSER
                     YRGKISTADLPMRNIAALYNYTRSATC"
BASE COUNT         8897 a         6247 c         5386 g         8588 t
ORIGIN      
        1 cttcttgacg cagtcatagg accgctgaat gtagcggcag agtatataga cagaatttca
       61 aaaggagata ctcctccgaa gattaccgat tcctaccgtg gagattttaa tgaaatcaaa
      121 aatttcaggg tctgtccaaa cctttgttgc gatgcgcgcg ctccggcgcg cgccacccac
      181 caacacacac tccgcaaggt aatgatccac aaacatggct tgcttccagg gtgacatatg
      241 agcaccttta atcgtactga gtcgacgaat cgcattcatc agcggcttcg gacccgagga
      301 aggtctgttt tgcatacctc taatcggttc acccttggct cttacctttg aaaaatcgag
      361 taccaacatt tcatttttcc actttttttc ataggccatt acctcaagca tttcaatcgc
      421 ttgcgcccat ccttcgcgac tatcaggcac ttcaaaccat ttgatgccat tttctttgcc
      481 atatttgtgg accgcatccc tgacactttc gtcgtttccc cactcaaaat ctgggtgatc
      541 ctgtgagatc acacagcgaa catttggcat gttgtcccaa tccaccacgc tcatatcgtc
      601 atcgtatgac ctaccaacac ccgagccatt catcagcaaa taaaacaaaa tatatgaagc
      661 agctgccgtt gaacaattag taaacacctc catgttgcgc gatggttgcg tttcatcacc
      721 atgttgtaag tgacgcccag acatcaaaat agaaccattc gtaatatgtt tctgtaatag
      781 ttcatattcg gtttggcgat gttttttctg gccagcagga atcaacagag tgttgccagt
      841 ggcaacccga cttgcaacct catcccatcg ttcccatcgc tctctttttg aatccttatc
      901 tgtgatcttc ctcaaatagg ttcgccgagc aaccgcttga cccatcccac tgtcaagctt
      961 tcgatctata aacttcattt tcttttttgg tagtgtgttg gcggctgcag tcatcataat
     1021 gaattaatac ctttctagtt actaaacaat ttttgaaatt tcctgtttaa aatcctcaat
     1081 atcattgaaa tccctataga cactggcaaa cagtaaatat gccaacggat cgagtttctt
     1141 caatcgattg agcaccatat tgccaatttc ccacgacttc acttcttttg aatctttgcg
     1201 acgcaatcgt cgttctacgt cttctatcag ctcatcgatg tctgacatag acaccggtcg
     1261 cttccaggta gcttttacta tccctcgttt gagtttttca cgagtaaaat cttcttttgt
     1321 cccatctttt ttaaccactt tgagctcaat accttcaacc cgctcatacg atgtgaatcg
     1381 tttgtcacac ttggcgcaca ctcgccgacg gcgaatcgct gtttgttctt caaccaccct
     1441 cgaatcgatc acattggttt cggtagtagc gcaatatgga catttcatag tgaaaaacta
     1501 cccattgtgt ttttgttaca tacaaaacac aagatgttgt agttaagcta tttttgggct
     1561 ataagaatgc cacacttagg gagatactgt caaccaatac atactatgtc aatctgattc
     1621 tattttagta ttttaattcg ctgtattcgt gttttttacg ttccatggaa aagcaacact
     1681 aaaccccaaa tggcgtgtga cagcatcagg gcataggcat tacgccatcg ataaaacacc
     1741 agagcttgac caagtgaaaa caggaataat aataaaactt ggctcatgac taatgcgggg
     1801 ctaaatcgta aaaacgcatt gggcagatgg aaaactagaa aaataatcac aatcagtgcc
     1861 atctgccaaa caagcgatac ttttttgtat ttgtcaaaaa ctaccatggc cacgaaacaa
     1921 tagaaaaata acgtttccca aaaaccagta aacagagcta gagcaaattc ataccaaaat
     1981 tgggtagact gaaataactg agcagattcc acgactcccc cagattggag caaactcata
     2041 gtggaggtaa taaagccata caacccaccc aaagcgactc cgagtaataa accttgttga
     2101 aaaatagaaa aacgaagtgg ttctaataca tcttttcgtt tggaaactac cgtataaaac
     2161 caaactggta acccaaaaaa gaaggctttt ccgagcgtct catcaaacca gactggaaaa
     2221 ctaaacagcc agcgataggc ataccaggct attacggcaa caagtagcaa agggaaaaac
     2281 aatacatgct cgttcagtag tttctctggt gtagtggcaa gattggagtt attgggttga
     2341 tttgatggca ttaagtacaa tgtttgtagc ttccaattga ctgactgaat atgtatctat
     2401 aactacatcg taaaggtctt gacgccaaaa atcaatcggg tccgatgatt ttacgcgatt
     2461 tggcgccaca acccaatctt gccactcttg ggcatagaca cgctgccatt tttttaagtt
     2521 cttttcgtag cgattattca tattggcgag agcgagatct ggagaaatgg catctctatt
     2581 gacaattcta tcgattcgca ctgccttatt gctacacacc attaatactt ttagtactcc
     2641 aggaacttgc tgggctaaga atccagataa ccaagactcc aatacccatt ttttttctga
     2701 catcagctta ctacgcatat ccatgtcaac ttcacgatca aattcatcac tatacacagt
     2761 ggcatcgtga tgaccaccag acgaagggtc aaagtaccct ttttcggtgg catatgcgcg
     2821 cataaattct cccccactaa aaccctgcca gccctcatct tttaacacta acttaagatg
     2881 ttgtaataga gtcgttgagc ctgctccggg taaaccagag atagtaatgt tttgatacat
     2941 aggtatttat tccttctcta atgaaatttg gcaggttact gcttcatgca tggtatcttg
     3001 cattcgctta agatactccc cggcttgagg aagtgtcaat acctggctat ctttgggaaa
     3061 ataatgaaag attcggttcc gacagcgcag ccaagtttcc cacaactcgc gagccaactc
     3121 ggctgagcaa tacctcacca cgtcttcata cagccagtgt tcatctcgat gtctcggaga
     3181 aacatcggga ttaagcgctc gaccaatccg aaactttcta tcattgaacg tttcttgcga
     3241 aattagctgc gtattcagta aaaaatgctt caaaaagccc tcataggctt tggacattgg
     3301 gaaaactaca aaggtataat cgtcatactg ctcaagtgaa agctcttggc gttcatataa
     3361 acgcaaagaa agcatcacca ttctctgcaa atctaaatca aggtaagtaa accaagtact
     3421 tgaggcaata tctggatgca tacaaaatta cgatatctct tacttttttg atacactaca
     3481 atacttctag tatagtgtaa acaattaacc atcaattgtc atgcagtcac ttcgcctctt
     3541 agagaaaaaa tactttaaac aatttaaaag attcaaaaaa aagaataaag gtaatttatt
     3601 tgtatctcta gttcggttct atttactcgg tggtagcccg gctgttcgtt catctttaaa
     3661 gaaaacaatt aaaactctcc cccacaaaca acggccactt acacgattgc tgaaactacc
     3721 aacaagaatc ataaaacaaa gatggtttca agcagttgtg ctgctcggta ttgctacaat
     3781 gattggcgga ttctggtttt tactccaaga cttgccatca ccacgcaatt tgaccagtca
     3841 agaaaactat cccgtcagta ctcaaatttt tgatagaaac ggcgtaaaac tctacgaaat
     3901 ctttgccggt gaaaatagaa ttccgattaa attggaatcg ttacccaaga atgtgattaa
     3961 tgccactatt gccattgagg atcaacattt ttatcgccac tttggatttg atatatcggg
     4021 gatttttcgt gcagtgatta ctaatctacg aggaaatcga cttgagggag gctcaacaat
     4081 cactcagcag ctagtgaaaa atgcactact caaccgcgac aggacgctcc aacgaaaaat
     4141 taaagaggtg ttactttcag tgatgaccga aacactctac accaaagatc aaattctaga
     4201 aatgtattta aactacattt catatggtgg cactgcagta ggaattgagg cagcatcgca
     4261 acaatacttt ggcaaaccag cctctcagtt atcacttgcg gaagcagcca tcctcgctgg
     4321 cttaccacaa gctccgagcc gatattcacc tttccagtcc gatcaatctg cagccaaaaa
     4381 ccgccaatct gaagtgctca gacgcatggc ggaagatgac tacatttcgg aagctgaagc
     4441 ggagattgcc cgaaccgata cgctcaactt tgccctatcc aaaacagata ttcttgcccc
     4501 acattttgtc ttttttgttc gggatcaact agtagaaaaa tatggtgtag aaacagtaga
     4561 aaaaggtggt ctgcgcgtca ccactacact tgattacgat ctacaacaaa ccgcgcaagc
     4621 tagtctgtct gctgagctag accagttggt taaataccaa gtcagcaatg gtgccgcctt
     4681 agtcgtaact ccaaataccg gtgagattct agccatgatt ggttctaaag attattttga
     4741 tccaaacgct gatggcaagg tcaatgtgac cttagctgaa cgtcaaccag gaagttccat
     4801 caagccaatt atgtatgcca cggcatttca aaatcgggtc ttaaacccgg gtacattatt
     4861 agttgatgtc aaaacctgct ttacagttcc aggacaaaaa gactattgcc cgaaaaatta
     4921 tgatggtagt ttccgtggac ctgttaccgt ccggcaagca cttggtaact ctttaaacat
     4981 acccgccgtt aaagctttga atgccattgg tatagaaacg tttatttccc aagcaaccaa
     5041 aatgggtatt acttcttgga cagatccttc gcggtatggc ttgtcactaa ctttgggtgg
     5101 tggagaaatc cgcatgatag atttagcaca ggcatttgct gtgctagcaa acgagggagt
     5161 caaggtacca tttagcccaa tactaaaggt tgaagattat cgaggcaatg tactgtttga
     5221 actggatcct gcggctagaa aagaagcgct cgatcatctc aatatctttg aagaagaaac
     5281 agaaaaaaac gatctccaaa gagtgatgaa tcgagcgcca gcgtatttgg ctagtcacat
     5341 catgcaggac aacactgctc gccaatgggc ttttggtagc aactctcaac tagtgattaa
     5401 aaatcaagtt gtctcagcaa aaaccggcac cacaaataat cttaaggata actggacagt
     5461 cggatttaca ccagaatttc tggtcatcac ttgggtagga aataatagtg gcaaaccaat
     5521 gaatccatat ttagtttctg gtattaccgg cgctgcaccc atttggaacg atattatgtc
     5581 gtatgtcctc aaaaaccgag agcccatgtg gcaagaaaaa ccgagcgaca ttgcttctgc
     5641 aaaaatctgc gccactggct ttcctccaac tgcgggtgat ggctgtgtgc cacggtatac
     5701 cgaattgtac tggcaaaaaa gtcaaccatc agaggtcaag ttggtcaaac aacaagtctg
     5761 ggtagatccc gccactggtc aaccaccccc tccaggtata tttgaaggcg aactaaaact
     5821 tgaggagcat atctactatc aagatcccgg ttctccactc caatgcgctg attgtgtgcc
     5881 tgttgatgaa cgatcgcaac ttgaattacc actatgagtt atcaaattca aaaacaattt
     5941 ctgaaggttc agaaaccgtt ggtctatgcc aaaaaaaaat ggcgtgggct caaaattata
     6001 ctatttctct gcgtgactat ttttatgggt gtatcaattt ttctttggca gacttggcaa
     6061 ccaccactca ccgctcccat ctcatccaca acaacctttt catttcttcc gtatgattct
     6121 gtcatgggta gcatcacgca accaaaactc atctatggtt ttctccccta ctggaatttg
     6181 gaaaaaacca cactgaacaa cagcctaact cacgttgcct attttgcctt gaccataggg
     6241 ggagacggta ctttaagaac tcaaggtgat gaagaaagtt tgactggcta ccgaaaactg
     6301 caatcagaaa gctttttaga cataattgaa aaaactcaaa aagacgcaat tcaattagag
     6361 ttagtcttaa agcaatttag ccctcaagaa acgactgcgt ttttactatc caagcaggca
     6421 caggcaaagt tctatgatca gttagattcg gtgatgctcg cgtatccttt tcggggcatt
     6481 aatttagata ttgaacttag tgatggccaa caactgcaat caaaattaac tgagtttgtg
     6541 acagatctaa atcgacattt agactctgtg gcgccaaata ccactgtttc gattgatgtc
     6601 tatcccagcg cagcacaagg gggaaatatc tggaatttga gtgagctggc acctcacgta
     6661 gattatgtaa ttgtgatggc gtacgatttc catcgcagac aatctgctca agcgggccca
     6721 gtgtctcctc ttttgggagg agacgtgtat caaaatatca atcagcattt aaaagagata
     6781 ctggagctag tgccagcaaa aaaaattctc ttgggcgttc ccttctatgg ctatgagtgg
     6841 caaactacca gtagagacga acaagcactc acctatcccg actctggttc caccgccagc
     6901 tatcagcgag ttttagatat cctggcccaa aaagaggaat taaaagtaca ggagcgttgg
     6961 aatgaaattg cgctcagtcc ctatttgtct tatgaaaaaa gcggtcaaac atttgtcatt
     7021 tactatgaaa acacgcgatc gctctcttat aagttagatt acgtcaatca actcaactta
     7081 gctggcattg cagtctgggc acttgggtat gaaggtgacg caaacgaact ctggtcggtt
     7141 atcaatgata aattagcact tccttgattt tttttcttgt tcatcaggct tggctatgat
     7201 agtatccgac ccacatggac taccacaaat tatctcaatc agaataccaa agagtcaggc
     7261 gagtactagc cctgcacctc tatcaaaaaa gactcgctct tagaggcaga tatagaactt
     7321 tctcagttaa caatgcaagc gacgggatta ttttttcatc ccaatcactc ctttctccaa
     7381 ccaaacgatt atttttatcg attcaacagt gtaatcaaat tttaataaac acgattcttt
     7441 ttttcacgcg accgttcgta tttcctttac gctactaccc gcttcataca attatcttac
     7501 ttactatctt cggtattatt tggggtttat tttataaagt gaacatagct atttttgtcg
     7561 atttaccaga cccgaattta ctatctgaac aacttcctta tgtctcaagt aaaataactg
     7621 accgtcatgg ttcggtattg tatcggctct atgatgaaga aaatcgcacc ctgattccct
     7681 tgcgggaaat ttcaaacagc gccattgagg caacgattgc aatcgaagat aaagattttt
     7741 ggcaccatca tggcttctcg ctgagagcaa ttattcgagc tgcgctctct aattctcaat
     7801 cggaactgac tcaaggaggt tctacgatta cgcaacaact agtaaagaac cgactcttga
     7861 gtccagaaag aacaattcag cgcaaaataa aagaattact tttatcggta atgacagaac
     7921 aacgctatag caaagaaact attctcacca tgtatttaaa tcaagttccc tatggaggat
     7981 caacctatgg tattgaggaa gccgcccaac gatattttgg taaatctgca tcaaagttag
     8041 atctggctga aagtgcgctg ctggcaggat tgccagcagc gcccactgcc tatactccgt
     8101 atggtaattc accagagaga gcatttgcgc gccaacgtga agtattgaga aatatggttg
     8161 aggctcagta cataactcaa gatgaagctg caactgctgc gcaacaacaa ttagcgtttc
     8221 gtcaagattc tatcgatatt atcgctccgc atttcgtcat gtatgtcaaa aacgagttag
     8281 caaaaaccta tggagaaaac attctcaatc gtggaggtct gactatcaaa actactctgg
     8341 atattccaac tcaaatgata agtgaaaaag tcatcgagga agaactgacg aagcttgcca
     8401 aactcaaagt gtctaatggc gcagcacttg tcacgaatcc ccaaactgga gaaatacttg
     8461 caatggtggg tagtaaaaat tactttgatt ttgctaatga tggtcaagtc aatgtgacgc
     8521 taagaccaag acagccaggg agttcaatta aaccaattac ctataccctg gcttttgaga
     8581 gtggttttac ccccgcttcg accattgttg atgccccaat ttcgtttact atacttggaa
     8641 gcgaaccata tagtccaaaa aattatgatg gtacctatca tggaccggtg actctcagac
     8701 aagcgctggc atcttcatac aatgtgcccg cagttaaatt gctctctgta cttggtgtaa
     8761 ataacttaat ttcacttgct gaacaaatgg ggataacaac ttggcaagat cgttcaaggt
     8821 ttggtctgtc attaaccctt ggaggtggtg aagtaacgat gttagatatg gtgaccgctt
     8881 acggcacgct tgcgaataat ggacagagag tagaaattaa tccaattctt gaagtaactg
     8941 atttggatgg caaaatcctc tacaacaata tctgcgctca aaatcccaca acctgccatt
     9001 ttcctcaagt tattccttcg ggaattgcct accaagttac ccacatactc aaagacccag
     9061 ttgctcgcat acctgcgttt ggtccatttt ccgttctcac catcccagga caagaagtgg
     9121 cagtgaaaac gggaactact aataatctac gagataattg gactattggc tatacgagtg
     9181 agcgtgttgt agcaacctgg gtaggcaata atgacaatac atcaatgagc tacgttgcat
     9241 cgggaattac tggagcctca cccatttgga ataaaatcat aagaacccag cttagcgctg
     9301 aaaaaccgca tgtttttcag cgcccagact ctatcatttc tatgaaaagt tgttttacca
     9361 accaagaaga agtcatcgtt gtcggccaac cattaccccc accctgtaac aagcagttga
     9421 gcgaacaaat tgatactctt atcgagccgt aatccaaagc agtgatccaa taatcaccgt
     9481 caataacaaa ataactggcg caatgatttt tgagagaagc atgcgccgat acgccgcagc
     9541 ttttgcctga cttttaagac gcgcttcccg caacttagat tctttttccg cttctcgaat
     9601 tcgactcaat tcttcaagat ctccagcttt tttttccaaa gaagtttcag tggatgtaaa
     9661 catacgctac ccagtttgtt gaaatcggtg atacatcatt ttggccttac caacaatgat
     9721 gtccacaact tgatcaatac ttaaatccga actatcgatt acccatgctt caggcaaaat
     9781 ggttagagga tctgttgtgc gttgactatc ccgcttatct ctctcaatta gttcttggta
     9841 aacctcctca aactcagcca gcattcccct ggtctgcaac tgaaactggc gtcgtttggc
     9901 tcgtacaatt tcgttagcag tcaagtagat ttttaactga gcgtccggca aaacccgata
     9961 ggtaatatct ctgccctcca tcactacatt ttgtgactga gcaatttctt gttgttttgc
    10021 caccaagaca gctctcactt tttgataggt tgcaactttt gaagctaaat caccgatctt
    10081 ctctgttcgt attgaccaag actcgtctgt atcatccact aagactgtcg tcagccgacc
    10141 gtctttttcc gtttcacttg ggttgcgcat ttggatagta actttttcaa ccaactcaac
    10201 taaggcattc tcatcatcaa tactcacacc tgctcgatct gccaacatac ctgtaactcg
    10261 atacattgct cctgtatcaa catacaagaa tcccaatcga tctgctacca aacgagaaat
    10321 cgttccttta cctgcggcaa ctggtccatc aatagcaatt tgaaatgatt gtgctgccac
    10381 aagatgatct actttctaac tacttcttct tacttttttt tgctggttta gatttcttgg
    10441 ttgtggtatc agagagccat tcggatctgt ctgctgtaag caagcccccc aaaccaacta
    10501 tgattaaaat taacggccaa aggttccaaa aatcttttgg tagcattcct acttggtctg
    10561 ctaaaaatat taatcccata acgaccatgg cagctggcca gaaatacgac tttgacataa
    10621 tcatcctttc gggtatgaca ggtacctata tctctattaa acagaagttt gtccaaaaag
    10681 tcgagaggta actcaaaacc cctttagctt acctttgatt tgactttcnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnat ttgagcattc aataagaaaa cctctccacc
    10861 aatcacctta acgtgactac ctttcagact tgcttgaccg atacgtacac tttttgtctc
    10921 tggacccgtc aaaacaatgc ctgcttgcca ggttttttct atggcatatt cttggaaggc
    10981 tattttattt ttgattaccg ccatatacct attataacgg catgcggtac aaaatagatc
    11041 cactcactac taaaactagt tgctgagatt catcataaac aatagtattt gccgaaccaa
    11101 gtgtgcgatt ttgaatttgg ttgacaactt ctccagtatc cttgttaagt acaactactc
    11161 ttgatttctt aggttctaaa acatacaagt tcggactttc aagacttgta gctaataaca
    11221 gtgttgattc aaatggctcc gataaaccag ctacagtaaa ttccacagtt cttcccgatc
    11281 gcattctagt gatttgacca gtattcgtac ccagccatat atctccatct actaccatgc
    11341 tgacactatc gccaaaagga acgccagaac tggatccaat ccaggttgcg cggttaacca
    11401 attttccgga ttttatttca taacggaaaa ttgctctaga ctcagagcta agcaagtaca
    11461 caaaattctc ataacctcca attaattgac tgttagtgac tgcatccgaa gtttctagca
    11521 attcattgat acctaattgc gaatcaagag tggaaagtgc aaaaacaccc cccccaagag
    11581 caagaaacat tttttcagta cttgttacaa cccttaactg gttggttgaa tctaaaacca
    11641 cttctttgcc aattttatcg gcaagtgaat atgaaataac atattgttgt tgagcatcga
    11701 gtgcaattaa gttgccgttg ttacttgaag aagaactagt tatgaaagta ggagaaattt
    11761 ttctcagatc atcaaaaatt ggtaattgtt cgattgcttg agattcatac agttgttgtg
    11821 ccaaattctg tgcctgttgt agagtcaaca gaacttgttt atgtgccgac tgaggttcca
    11881 aatattctga ttctaaagtg gataaattct gaattagttg ctgcacgccc aaatatgctt
    11941 gtgcgggagc agttttggcc tgtttttcta aatccgacag cgtttgctga tacggaatta
    12001 atttctccgc gagctgttcc tttacctcca acgttttata gtgagtcaca acaacggtaa
    12061 ttgtgactaa taaggtagca atcaaaaaaa taatcaatgc aaccaattgc ttcctaggaa
    12121 tgtgtacccc tacgtatgtt tttgcggggt gcaacaataa ttttactaaa gaagttcttt
    12181 gtacgttttg ctcacttttt tttgattttc tcaaaattga accaacaaac caaagtagaa
    12241 aattaatgct tgatttaatc gcttctaaac caaaagtgag accagatttg aatatattca
    12301 gtagttgatt ctgtttctct ttcaccgcaa ctgttggcaa ctcattagat tcattttcag
    12361 attcctttgg tgaagcaata aaagccgccg ccattgtctc agaatgattc attttcctaa
    12421 tcgctcgctg aaattcttga gcaagtgttt gaatttgatc caaagcaaaa cccttggaaa
    12481 acaataattg gatttgtttt tggacactat tggtataaat cactaacaca tctgcttgct
    12541 taaatacacc ttgtagcacc tgcactattg gttgcgaagt caataatgcg cctctttttg
    12601 atcctctcac aagaaaaaca ccaccgttca aggaagctac atacaatttt tgattttcaa
    12661 taatcactcc aaccaccgaa acatcgacgc tattttcttg gcaacgtgtt tctatggtgc
    12721 taacaacctc aaataaactt tgagcatccg tcatcgtggt actttggatg aatttctcta
    12781 tggaagcgga ggtttcacgc gctgttttac caacaacgga aaaaatgaga caagaataat
    12841 tctggttagt agatttttcc caaaatcgag actcgccttc tggattgggc agtcctagtg
    12901 aaaacttact atgtacgatg gccatgaatg tggtttaaag agccagataa tatattacaa
    12961 ctactacaga gagcagaaag tgactaattc caactaacat gagggcggca gaaaaaccag
    13021 tatggactgt ggttctcatt agttggattt gtcggataac tagcaatgcg aaaagcgcat
    13081 agagcatccc gccaaccaca aagagcattt tgacaaatcc caaaagtaat tggttaggat
    13141 cttgtaatat tccatcaaaa attggtgcaa tcgtgggatt catgaaactc tcccttccaa
    13201 tttttcgcgt tcttcttcga gaattaaatc attaattagc gtcgttacct ttgaaggatg
    13261 aggtatccct gcgaattcaa attctcgctt ctctgcagct gtttgaatta ccactgtgcc
    13321 aaaatcaaag atggctgata gaaatccagc attcatagca gtagtgtctt cgatgttatc
    13381 tattttggca ctcgaaatat ttctaaagag taagctgttg aaatcaacat caataattcg
    13441 ctcatcagta atgatgtaaa cattgaaata ccatttcaag aatgcctcaa caataaaacc
    13501 gagtaagaca agaaaccagc ccaccatggc agccatttgg tagttggctg gcaaaaaatt
    13561 gaaaaaatca attttttgca agaggaacgg gaaacccacc ccaagcaaag ctaaaatgat
    13621 ccaccaaacc tgagtcaccg gatgctgacg aagcaagagt atgacaacct cattgggcgc
    13681 ttgggtacta aatttaagtt tatacggttt tgccacaaat gatgcaaatg gactatgggt
    13741 tgtttccata tctctcatta aatgagaata ttcagaaaca tctctggatt tgtgagtact
    13801 ggaaattact ccattggtat ctaaaagatg accttcttga tcgactcgat gcgcaagacg
    13861 atgctttcta tgaatctgca cccgttccgg aggtgtacta gaactactaa aaatgtctgg
    13921 catacaacct attcattata tcaaagattg cgcttattgc ttgagaagtt gcaaaaatgt
    13981 ctgctgatta atgctaattg agccaaactg cttcatgcgt ttttttccct tggcctgttt
    14041 ggccaacagt ttttttctgc gagtcacgtc acctccatag agcttagcgg tgacgtcctt
    14101 ccgataggcc ttgagtgttt ctctggcaat aatttttccg ccaatggctg cctgaattgc
    14161 aacttcaaac atctgctttg gcagaatatc tttgagtttt tcaaccatct tgcgcgctcg
    14221 actttccgat tgcggacgaa ccgttaaaaa acttaatact tcgattggat ctttgtttag
    14281 cattattgat actaggacta catcagctgg actgaattca ccgactgaat aatcaagaga
    14341 ggcaaatcca gaagttgctg attttaaacg atcgtggaag tcagtaatca gttccgcgag
    14401 tggcatttta attgtcagct gctgctgacc agcaattggt tgggctgaaa taaacgtacc
    14461 tctgttccga cgtaccaatt ccaagcattc atgctggtaa gctacaggag taataaggct
    14521 aacaactgca attggttcta acacctgctt tatttgggag ggatcgggaa attgtgatgg
    14581 tgcagttacc atcaaccaag ctccatcagt tgtttgcacc ttatagtgta ctgagggagc
    14641 ggttgctatt aaattaagat cgtattccct ctccaatctt tcgagcacaa tttctgcgtg
    14701 caaaatacct aaaaatccca ctctaaaacc actgcctaac gcattggagt gagctggagt
    14761 gtgttgcaat gcagcgtcac gtagtaccaa tttttctaat gattctttta acaattcaaa
    14821 ttcgtcagat tcaacggggt aaatctccaa gtacaccatt ggagttggag gttgatatcc
    14881 caccaatggg gcaacctttt ctttaaccgg cgatgtcgtg agcgtgtcac ccactagtaa
    14941 accgcgaaca tctttgagag atgtaacgca gtaacccact tctccagcat ttagcgctgc
    15001 ttgtggttgc atttttggta caaaaacacc cacttcactt gggtgaaata ccgtttgact
    15061 acctaatagt cggactgtac aaccagcagt aaaactgcct tcaaatactc taatcagtgc
    15121 caccgctcct cgatgttgat cataatgaga actaatcaat aaggctttgc cctccgttgc
    15181 aggaaagact tttggcggtg gcaccctttg tacaattgcc tgaagtaccg cttcggtatt
    15241 gatgccggat tttgctgaaa cacagattac ctgatcctcc ggcaaacctg ttagctccat
    15301 aacttcgagc actgtcttgt ctacatctgc ggaaggtaaa tcaattttat tcacaactgg
    15361 aataatagtt agacctaagc tatgcgccag ttggtaatga gaaagagttt gtgcctgcaa
    15421 tccctgtgag gcatctacca ccagcactac tccctcacac gcagctaaag atctacttac
    15481 ctcataacta aaatccacat ggccaggagt gtctattaaa ttcaatatat attcttgttg
    15541 agattgtggg tcaaaatatc tcagacacac cggggcaagt ttgatcgtga ttccccgctc
    15601 gcgttcaatt ggattgctat ccattaccct aacttcattt gcagtgattg cattagttaa
    15661 tcgcaaaaaa caatcggtca aagttgtctt accatgatcg atatgagcaa taattgaaaa
    15721 gttacgtatc cgcattagta cttcaagcta cgcagctttc agtaacttgc tggcttgtgc
    15781 tttgagacga gctgctttat tgatgtgtag taaatttcgt tttaatgcac gatcaattct
    15841 ggagtatgat aaatccagta actctttggt gggagttgtt ttcagctgtt tgagcacact
    15901 cctcatgcga gaacgcacta ctgcgttgta agccgctttg cgctggctaa ctcgtaaagc
    15961 tttttgagca ttttttagga ttggcatata taatgggggg gattatatca tgttcggcag
    16021 atttaaacta ccaactttac tctctaataa tactaagtgg ttagagcgcc aacagaccac
    16081 aatactttct gcggcactca ttattggtat tgccaatatt atttcatcaa ttgcgggctt
    16141 gattcgagaa agattactga ttagttattt ttttgatact tcagaatcca gactaaattt
    16201 agaagcattt caggtcgcct ttcagatacc agacgctctg ttccaacttt tagtacttgg
    16261 agcagtttct gcttcattca taccgatttt tacccatcta aaaaaaagag atagtaaaac
    16321 cgcctttgct agtgcctcaa tcattatgaa tgtagtactg ctggtttttg tgttagtaag
    16381 cgcggtgatc tttgtttttg ctgaaccgct tactttgttt cgcactggta gcggtttcac
    16441 acctgagcaa cttgcggttg cggttcagct aacaaaaatc atgctttttg ctcaattctt
    16501 ctttgccatc tctaattttc tatccggaat tctgcaatca taccaacgct ttatagtgcc
    16561 agcacttgcc ccgattctgt ataacttcgg aattattctt ggtgttgcct tgttctccca
    16621 aaattttggc atttatgccg caggcatagg ggtgattatt ggcgcactgc tccatatggc
    16681 aatccaacta ccttttgctt atctacttgg tttccgtttt actctctctt ttaacttgtc
    16741 atcaccagga gtaaaaaaac tcttttcgtt gatgccccct cgattcatta cctatgctat
    16801 caatgaatta caacaacttt cactcggatt ttttgcaaca tctctgggaa acctcagttt
    16861 ttttgtaatt cgattagctc tgcgactcat gacgattcca atcaggattt ttggggtacc
    16921 aattggccaa gcatcgctat catttctttc tgaagaatca accgatgaag attacaaccg
    16981 atttagatct ctcctaactc aatcgctcaa tcacgtagca ttcctggctt ttccagcgtc
    17041 gatgctctta ttaatattgc gaattccaat cgtacgtctc gtatttggtg ccagcaatct
    17101 accctgggaa accactctag caactggaag agtagttgca ataatctcac tttcggttgt
    17161 tgcccaagct ttagttcagt tattgatacg ggggtttcat gctctgaatg acacaaaaac
    17221 tccgctctac attaccctcg tcacggcaat tacctattta attggctgtt ggttctttgt
    17281 cttttatacc cagatagggt tattgggcat tgctctagta attttaatta ctgcgttttt
    17341 agaaatgttt ctctgcctat ttttacttga tcgtaaaatt ggtcaaattc ttaccaaatc
    17401 atttctcttc cctcaagcta aaattttgct tgcaagtttt ttcatggccg ttttcttata
    17461 cttaccattc aggatactcg atgaattgat ttttaataca tctaaaaccg ttgagttaat
    17521 cgggttgact attaccacaa gctctatcgg gttgttagtc tacctttact tttctgcatt
    17581 attgaatgtt cgtgaattac aactggttgg atctaccttt tcttctcttg atcgctggag
    17641 aaaaacgctc tctcgtacca cggaagtgtt gtcagaaccc acgaatgaag aaggattgcg
    17701 gtaatcatca gagcaacgta tctgtacaaa caaatacgat tgtgtgtaca atggtctcaa
    17761 cctgcctatg ccaaagcgag cgacgacaaa aatatcgatc aatttgttgc ctgaagatga
    17821 tttcttactg ggtagttttg gcaaagttat ccgttgggct gtatctgtag gaagatactt
    17881 agtaattttt actgaggtaa ttgtcattat tagttttgct actagattta gcttggaccg
    17941 caaggtgaca gatttaaatc aggaaattaa taagaaagtt gcaattatag agtcgtatgc
    18001 tggactagag tcagaatttc gttcggcaca agaaaaactc agtcagtatt ctgagctcga
    18061 aaaacagcgc aacttaaccg acgtatttga gcaaatctcc aacgtaaccc cgccagatgt
    18121 agtcgtgacg caactgaatg ttaagaaaga cggtatcagc attactggat caactttgtc
    18181 acaaactgcc tttaaccatt tgataaataa tctgcaatta tcccctttct tttcagatat
    18241 cagaatagat agcgtagaaa acactcagga ttcaagtggc tacgagtttt cactccaggc
    18301 caaatcaaaa aatgccaact agacaacaaa catcactctc tatttcaaat ttctaccgac
    18361 atccaatcgc agcggtctct acagaattac tgttcacgat tgtatttata cttgttttag
    18421 ccattgttgc catccaacca accttaaaaa caatggccga tctttccaaa gaaatcgagg
    18481 ataaaacgaa actcaataaa agccttcaag ataaagttgc tgcattaaat actgcacaag
    18541 ccgagtttta tcgatggcaa gcccaactcg ctttacttga tactgcgatc ccagataatc
    18601 aacatacaat tctagacgta aagttgtttg aaaaactggc ttcagaaaaa aacgtagtca
    18661 tcggcaagat tggcttatcg gagtatcccg atgcaactaa accgctctca caaaaaccaa
    18721 tagtcaatga tctacctata tccatcacta tccaaggtga ttatatttcg ataaaaagtt
    18781 ttgtttccgc attattgaat agccgacgag tatttgttat aaattctatt aatttttctg
    18841 tctccacttc taggggtgga caacaggatc ttacggcaac cctcagtatg aacgccccat
    18901 attatcaata aaaatcgatt gtttatgaaa atgccactat tagatatctt cgctgaacga
    18961 actaaaggtt tttttctaat ccttttaagc cttctctttg tgtgtgtaat tttttggatt
    19021 atcatttcat tgttttcatc ccaaaaaact acaaaagtcc cagaatcatc tcaaaaactt
    19081 gccaccccac tcaatccagt gcttgacaca tcaaaactgg atagattgga aggaaaaacc
    19141 tactatactc cagaagaatt acagtctttc ccgatcaata taattgagac cgatgcaaaa
    19201 agtcggacaa agcgcgttat tcaaattcaa cccacttagt tcggcttttt ttcgcattgc
    19261 agtttagtcg ttttttctct acagtggtta atagagatat tatatgttaa cttcaaaaat
    19321 gaatggtgcg gttttagcca agcgcatacc tactttgttg ggtttggctg tgttggtggt
    19381 tggcttaata gttggcacaa tagtttttgg tcaaggaacc ggggtcttct cacccagagc
    19441 tactccagaa accacaccaa aacaagttaa ggttaccaac gtaagtgatc gagcattcag
    19501 cgtgtctttt attacagaag aaagtacgac tgcgttcatt aagtatggga cagatccaaa
    19561 caagctatca cttcaagctg gtgacgatcg cgatcaactg tatggaacaa tcaaggacta
    19621 caatcttcac catatttccg cgacttctct agaagataga acaacttatt actttgtaat
    19681 tggcactgat cagggcgcgc tgtatgacga taacggtgaa ccattttcca tattaactgc
    19741 agcaagtgga ggaacgccac cagacgcaaa aactattttt ggctcagtta caactgctca
    19801 gggaggaccg gcggcaggaa gtattatgta tgcaaaactc gagggtgctg gagagttgtc
    19861 tcagctagta caagattctg gtagttgggc gattgtgctt cccaccgccc gtacagaaga
    19921 cggttcaagt tatgcgacga tcacaacttc aaccccacta gttctattag ctcagggatt
    19981 cccacaagca ctcacggcgg cactcaacac aactgttgga cagttcgaaa tggggtacac
    20041 atttacccta ggtcaatctg gaggtggtgc ttctagccca actgctacgc caaccgcagc
    20101 tcaagtttct ccatccccaa ctgtaacccc caacctatct ggctcaccca ctcctacgcc
    20161 agtagataca gggtctggcg gaggtgggtt gggagaatta ctcggtgaat caccgctccc
    20221 aactcctaca ccaacagaag caattaaagt tgatttaacc gcccaaggag aacaaatcgt
    20281 tacgaccgaa caaccaatca tcgttggtaa cgctccagca ggagtggtgg taaatttgaa
    20341 ggtaaattct gatacccaaa ttgagcagca ggttacaaca aattcacaag gacaattctc
    20401 tttagattta gaagctcttg gagcccagct tgagcctggt actcattctg tcgagtactc
    20461 ttatacagat cccaccacag gaagattggt cactgaaacc aaaaccttta ctgtatcaag
    20521 tggaggggta caacttgctc aggctaacat cacaccttca ccaactcggt cactgtcacc
    20581 cacagcatca ccaacaccaa tttcttatgg tactgactat ccgtatggca cctcgccaac
    20641 acctaccact agttacgcaa ctggcagcgg acaacaagca tcagtctctg ccacccccac
    20701 tcaacccgtc tctaccagaa gcggaatgcc atcaacccaa agcggagtgc cagtctctgg
    20761 atctgtgggt acaacattag tattactttt tggtggagcg tttttcctaa tttctggtct
    20821 ctggagtttt tggattgcca aagaaataga ataaattccc tctttcgtac tataatccta
    20881 ttctgtgaaa aaaaagaacg atatgattct tgttcctaaa tacagttcat ctttccaaaa
    20941 aagagtgttt gatttgttgg ttggaactag cctactactc tgcgcaatgc ccgcaatagt
    21001 attgacagga actgcaatcg tgattgcgtc aggttggcca gtcttttatt ctcaaccaag
    21061 gttgggcaag aacaaacaag tatttacttt atataagttt cgaactatga aaattggagc
    21121 cgacagtaga caaaaacagt tttctaaact caacatcgct ccagctccta tgtttaagct
    21181 aaaaaaagat ccccgattca catcgattgg ctactatctt tctcatactg gtcttgatga
    21241 actaccccaa atatttaatg tcttaaaaaa cgatatgagt ttggtgggac cccgaccgct
    21301 accgattgct gagggcaagg cattgaaaga cgattggaac tggcgctggc aagtgaaacc
    21361 aggattgttt tcttattggg ttctttcacc ccatagatat cagagttagg aaaaatggaa
    21421 gcaattagaa aaagaaacaa ttcaaatttc gaatttacaa gctgattgta ttttgatggt
    21481 aaaaattctc agcagacagg ttaaatcact agttgactat tttcccagca tcttccaata
    21541 gagcaactca acaggcgctt tatcatcggt taaaaatggc aattcgtcat caatgtttac
    21601 ttggtgaatt ctctcccgta tggttgtagc cacttcattc accgctgggt cagtaaaatc
    21661 tagaatattc tcaacgtcac caatctgaga atttgaggcg taaatcatat tattactaat
    21721 taaaaactga ttgtctctaa caggtgcgtg ataggtatag ggaaaaatcg atgcaacggt
    21781 attgacaatc acttgaaata acgtagggcc atcttgagct agttgtggac taacagcatt
    21841 gaaaaccata atcccatcag gctgtaatac ggttttaatt tgagcaaaaa actcttgagt
    21901 catcagatgc gatggcgcat ataaatcggt actgaaagca tcgattagca ctacatcata
    21961 ttttttctga gttgtagcaa tatacttcct tccctcttca ataataatat ttgcttctga
    22021 gtctgataaa gcaaaataat cctttgctac ctcaatcatg gtggggtcaa tttccaccgc
    22081 gtcaatttca actgcatcgg gaaaaaattg attgagttgt ttgattactg tacctccagc
    22141 aacaccgatt aatagaaccg agtgaggttt ttccttctgt aacaagctag gagcaagtgc
    22201 taaataatca agataattat tcattatgcc cgactcagga attgaaattg tttgaacacc
    22261 acgagcatta cccgtactga ggtacagtct cccattgtgg tcagaaattt taatatgatt
    22321 gtaaagcgac tccatcacca catcagcctg aactttaatt acaaaaacct gatatatacg
    22381 atccacaagc acaataccaa ttatcaaaag tgcaaccaac tgagaagttt tacgattaaa
    22441 aaccactagt aacgaactaa gactgagtaa aattgaaaac aattgtattg aatgcaagac
    22501 gccaattgtt ggaattaaga taaatgatgc cattactgaa ccagtaatcc caccaaaact
    22561 gcctaccatt gataaaattc cggctgtatt accaatgctt ttaagcccct tcactgagag
    22621 cttaagcaaa atgggactga ccatgcccaa aagcgtcatt gcgggagcaa atgaacagag
    22681 catggcgatt gttaaaatta agagataaag ccaggctgaa gagctttgag ccacaaattg
    22741 taatgactgt tgcaacaggt attgactgct aaaatagctc aaaaataaaa taaagcctga
    22801 caaaaaaaga gttaaagcca gtgttgttgt cggttgtttt gttttgtctg ccaaacgacc
    22861 acccaagaaa tatcccaaag atagtgcagc cattaccaca ctgagaagaa taccccaggt
    22921 gtaaacagag ttaccataaa acggggcgat taatcgagaa acagtgagct cgtagcccat
    22981 gataatcaat ccgataagaa aactaatcag agagtacgtt gtgatgcgag ctttcataac
    23041 acaagattat agcgtagctg tcattctatt acttaaattt ttatcagctt aaatcatcgg
    23101 cttaatataa cagcaatttt tcagtaatgg accagtaccg attcctatcg ggaatattgt
    23161 tgactattct tgctaacccc tcagaacatt cttggtttag ttgttgagat actgcaagac
    23221 aattacgata ttctttttca gcacatgtaa ggttcattac gttttcacat agcaaaccgg
    23281 tctgagaaaa agcgggagat ttttcgtcta taaacttagt cgcactccat gagaggttat
    23341 atgcgagatc aatttgatta tctgatagcg aaattctacc caaattagtc agatactttg
    23401 caatgctcat actttcttct ctgtctagct cattgaaaaa cgaaatgttt gaatttcgtt
    23461 tcaaaatctg tgtcatctca tcagaaatca taaataccca ggatctttga ttatgattat
    23521 cataatttga tagtggatta tgagggtcac cttcaatagt ccacagcaat ctataccaga
    23581 gagccagatt aatagtatca gtcagttgcg atgcagattt agttatctct atgagttgcg
    23641 tgctttcaga atttgtacca ctcaacatga ttgccaatga cagataatag tcagagtagt
    23701 accccgcttg gctacgaaaa tactctggcg atatcgtcct caaatgctca tagtatgtat
    23761 attcatcccc atccatcgca actctgtttg caataattgg tgtttggaac gacaaggccc
    23821 acggatctag ttcatatgcg attcggtgat acgcgccagc ttctccatac tctttctgtg
    23881 aaaataaata atcaccaaat ctaactattt ctggagtaag tttatataat tgccaatgct
    23941 ttagactatt tgttttttta agtcggcgag tgttctcaat cacaagatta atatatttct
    24001 ctgctgattc gcgctggtta ttttcaatat agaaatcaag agtgcttcca gcaagtgaat
    24061 gccaaggcga taatcttatt aaattataat gtgcgttaat tctatctgat tcggattccg
    24121 cattatcatt tttatattgc cagtagtaca ggtcattaat aaaatagtta tcaacttttt
    24181 ttacacaatc ggtagatatt ttgttgtttt ttcctgacag aagcaattca tatgcctgct
    24241 tgttaccagc aaaaaccaaa caagcataat ctggtttatt aatggcaatt aatacagctt
    24301 gagtaatata aattaaaata aaaaacgaga gaacaaatac tagtagcctt atgactacta
    24361 gtagactgct tatgttactg ataaatagaa acttacccgc tttatttgta tttgcactac
    24421 tctttgatat tacaattcca agtaccatca gggcaagaag aaatgaggtt gatgtgttcc
    24481 agtcataatc aagtagtgca ttgaaaaagt taaataggag tccaattact aaaaattgct
    24541 ccaatcgatt gctgttttta ctatttctta tagatgttag gatagtagta aacaataagg
    24601 taaataccaa aaagccaaat gttcccaatt cggtaattat ttgaagcaaa taattatgtg
    24661 cataatgtga atatacccca ggctgttcag tatattttct ggcgattaag gagtatgtat
    24721 ttaatccagt acccaatagg ggctcatcaa tcaggttttt cactgctact gataagtatg
    24781 attttctggc tccaatttct aacggtttac accaccaact cgtacttccg taacatacat
    24841 ttacttttgt agaaaagatc agaaaccaga aaatcgttgc tgaaattact attattgaac
    24901 taaggaacag tattttaatt ttattagtta aatgattagt tcggatcaaa agtataaatg
    24961 aaacaataat ttcgattaat cccaagaaca atagtattct tccggagcta ctgattagac
    25021 ttatgagtga gaaaataatc aacatcgcgc caagcctatc aagtttttta ttgatcaagt
    25081 aaaatgctgc cggtaacacc aatagtacaa ccgaacttaa atgattatga ccataggcag
    25141 gtaataagaa gtcggtagat ccacccagtt ggttacttac tggtaataag agcaatacaa
    25201 tatttatcga gattaatacg acaatatatg tactcatcgc cacaaatact ttcatccgac
    25261 caaacttttc gcaaataatc agcacataga cgaaatataa aaagtatcca atttgatcaa
    25321 gcaatcctga taaagaaatc ggaacggaaa tagtccagag tagcgatgcg ccacagtaaa
    25381 tcagataaag tatccaggaa aaaaaaataa aaaagttttt tttcagaact cccaagatat
    25441 tctgtcttaa gactattagt gcaataacaa ctagaaacca tttcgctgga tgagtcgtaa
    25501 agatcgcaat gaaagaagtt gcgactagta taaataaatg agcgtttttg ctaactaact
    25561 tatgcatacc tctttcaaga ataattcaaa atacaccaaa tactataaca gctaaaatag
    25621 ctcttcgtga atcttcatgg aatgatctaa tacttagtta actttttcag agtctcttat
    25681 ttaaaacttg caataaagct gtcgacgacc tctactccac cattcatcct aatttttcct
    25741 ttttctaacc agatcactct attgaaattg tcctttataa agtcaagcat gtgactcgcc
    25801 aaaattattg ttttcccgga attaaataac tcatgtatct ttgttgttgc ctttttttta
    25861 aagttttcat cacccgcagc gatattttca tcaattataa aaatgtcagg attcgagtga
    25921 acacccactg aaaatcccaa tcgcaacttc attccttctg agtatgtaaa aaatggctga
    25981 tcaataaact gccctatatc ggcaaaagaa atgatgttat ctagttgttt ttttacctca
    26041 gtttttttca taccaataag caaagcgttg ataataatat tgtccactcc gctcagatct
    26101 ggatgaaatc cagccgcaag gtcaattaaa gaaactgctc ttccagataa atgtatgttg
    26161 ccagatgtag gactagtaac tccagccatt agtttcagca ccgtagtttt tccagaacca
    26221 ttaactccaa taaacgccag cctctcaccc tgttttacct caaaggaaac tgaagaaagt
    26281 gcagtaaatg attcgttttt ccgattatga aaatatacaa aatctgacaa agttggcttt
    26341 tcatgtctaa aaaaatagct cttggtaaca ttagaaaaaa caactgaagc tcgatttttt
    26401 cgcataacta taaccaatcg tcaaaggatc tactcttcat tttgaaaact aatgtaccta
    26461 acaacaacaa tgtcaaagac accactatgg caatagtcgc attaaatgta gaaatgtttt
    26521 gactgtttcc agataaagct aattgaaaaa aactcacaat ataacttaaa ggattggcat
    26581 ctacaataga ttttatattg actggaagct ggttgttaga atagataatt ggcgttgagt
    26641 aaaacaacag ttgaagaagt atcgatacac caaaactcgt atctctaaac ctgacgttaa
    26701 ttgcagatgt tagtaaagat aatccaatag tagttacaaa taataaaatg attcccaaaa
    26761 ctaaatggaa aatatttgtc tgaaccccat taacaatcga aactactaag agtagtgtta
    26821 gtccaacaat aaagtgaact aaatgggcaa gactaattga tatgactatt atttctctcg
    26881 gaaaccgtga tttctttatc atatgtctct cattaactat tgcctgagta ttctttagaa
    26941 ctgtttgaga aaaataattc caagcaagca atcctgacaa taaatacaag aaatagtttt
    27001 gaatggagtt agagagtaaa tgttgaaaaa ttaaccctat taccaacatt tgaaatattg
    27061 gggttaccac tatccatatg aaacccaact ctgtcctttt atagcggacc acaagctctc
    27121 tttttgtcat agcctcaagt aaatcaagcc atataaaaga gttcttcaaa aaagatagcg
    27181 attgaaatcg caaatattgt aaacgtgtgt atgaagttgg tcttttcatt aatgaactat
    27241 ttgatagtgt taaacaacca agtaaataat ttacactctt cttatactgt tgtagacaac
    27301 taaaacattc tcaatatact agacatatta caaaaaatgt caattgtcca agccgatcat
    27361 cttgagtatt tttgaatagt ttcctcatct ttatgactaa caagcgctct ttgtcattgg
    27421 atgtgattat tgttactaaa caaagatact cacaactgtt agagtgcgcc acgcgtattt
    27481 gtaacaatac tcaaaaacca gccaacctca ttatcgttga tagtagctca aaaagagacg
    27541 aaaaaattct gttaactatc tctaaattat gcaagttgaa gaaaatcaag ctaactatga
    27601 tcgaagcatc agatcatgga atagcctatt ctaggaatct tggtataaga agtgttaaat
    27661 cgacgcattt tgccttcgtc gatgacgatg agtatgtcac aaagtattgg tgtcgtaaaa
    27721 tcttaatttt ttttgaaaat aatcaagact accaagtttt atgcggtcca aaaataccaa
    27781 caaacttgga taattactta aatcaaactt gggcatcgat ttttcttccg tcactctcgt
    27841 attcaggtgt tgcaaatttt gttccctctg ggaattcatg ctatcaaact aggttcatta
    27901 gagaaaatca gttattttat gatgaaagat tttcaagttc ggctgaagct catgcaatgt
    27961 attcaaattt gatacatcac ggcgctcaat gtttctttct ggctaattta tgcatactcc
    28021 acgattggaa cacatctctg actacattca ctaagcggtg gtttgagtat gggaggggga
    28081 tccatttctt ttttcatgaa tatcttgcaa gtgaattctc aggaagtctt cagcgctatt
    28141 tgttcctagc acctgatcac ttattcaggt tttttccgtt tagaaataat agacttgtcg
    28201 cacaatagga tttgatacga ttatgggcag tacttatttc taggagtcct gcccatgaat
    28261 ctaacctatt ttctcaaaca tcccgaactt tatccagcag caattggcat ctctcaaacc
    28321 cagtttgagc agttggtgcc agattttgaa aaggccttca ctaagtgctg gtacagaaag
    28381 gcttggtcgt atcaacgaca acgcggtgtg ggaggtggca ggaaacccct gcttaagcac
    28441 actagacaaa gactattttt cattctcttt tactacaaga cttatccgac tttcagatta
    28501 gcatctattc tgtttggagt agataaagag accattcatc gatggaaagt gtttttagag
    28561 caagtgctct ggatggctat tggtggtcaa ttaatactac caaaaaagaa gattaaaact
    28621 ctaactggat ttatggagat attccctgat ctaaatgaat ttatcgttga tgccactgag
    28681 cgaagagtct taaggtcaaa agatcaatct actcaagagt tttactactc aggtaaaaag
    28741 aagtgtcaca ccattaaaaa ccaggtcatc attcatcctc ggactaagcg aatcctagca
    28801 gtatcaaaga ctgtggaggg taaaagacat gataaacagc tttgtgaaga tgatggctta
    28861 atacttcgtg ctccaccagg agctgttggt agtggtgact taggttatat tggagtttgt
    28921 gaactaaacc ccttgattaa ggtggcacta ccactcaaga aaccaagaag tagagagctt
    28981 aatcctgaag agaaagagac caatcgagta atttcgtctg tcagagtaag agtggaacac
    29041 gtaatcggag cattaaaatt ctttgacatc ctctcagagc gataccgagg caaaatatcc
    29101 acggctgatt tgcctatgag aaacatcgct gcgctgtaca actatacgag gtctgccaca
    29161 tgctagtcac gatttgggga gggggagaaa acgagagtta ttttgcgaca agtctaat
//
LOCUS       DAOQZP010000004        27164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_2072_length_27164_cov_6.234645, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000004 DAOQZP010000000
VERSION     DAOQZP010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 27164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27164
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2072_length_27164_cov_6.234645"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PKH60_00420"
     CDS             <1..544
                     /locus_tag="PKH60_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16547.1"
                     /translation="SGIQLPSSEITQIGAPNHWANTWDSITDNGRMYHSSHKGDCLVA
                     VAISVVGRPNSDSQHATAIKRKSCPDHTPSQVETYCRSGQDPVLSFHAKAKAGLETGA
                     AVQKIVPNGRPADWEACGYSVIMEYPPLTQAPTISNSSSKGTQLLESALIYAFQHGIT
                     KVYAYSRPVGLQQHLASSLS"
     gene            1652..1918
                     /locus_tag="PKH60_00425"
     CDS             1652..1918
                     /locus_tag="PKH60_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ16548.1"
                     /translation="MSNNVVDERRTGRADDANKLDMRNLPDLLTVREVADLLRVSPLT
                     IKRWGKRGKLPAIRINSRGDRRYKKEAVLWLLGITPEDAAEAMA"
     gene            complement(1919..2677)
                     /locus_tag="PKH60_00430"
     CDS             complement(1919..2677)
                     /locus_tag="PKH60_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16549.1"
                     /translation="MSQLTTIDIVLPENSSSFFNDLTTFLRDMGELRPQSLLFHTTKR
                     SYYPLISLKEDVVEQPLVRFTMSDEEVYEVVVENSSGTEKKSQYAYTPIAMVDFVARI
                     QAAALQNVDHLGFNLPWFKAGVHPKIAALRDQLKTKCLYHTFPSGEPWDFILPGTQAE
                     VEGKKPVDYIQIRKPKFEIVSFANCSKPLVQIDLAFATPYEKLARLFPEGILLPDVKS
                     VWVYLQNPYDIDVCLVLNKAKGDWSNYFRSSRVT"
     gene            2902..3753
                     /locus_tag="PKH60_00435"
     CDS             2902..3753
                     /locus_tag="PKH60_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16550.1"
                     /translation="MDQTSNQTFSEPMDSLSETQPQKPYKLIGLAVAIIIIAAFLVFL
                     GSTRITTKTTQLQQPLESQQPTETETYSIDTQEPEQQKVIDPDTVEWKTFVSELGFSF
                     DYPSHWGTPTEKVLDAVEALQGDTGKTYQLTFSNTQEVYASGRSADFSAGRGGRQEDF
                     GVNSNGKTSIEAYLDIVDPGCFTMGNQNSFRALIDFSLVDKPVGGVRLFLPIVSQSIL
                     EKIKEEFSIPSETIDCFSDVEAQNQLIEKGKEIRSMLVANTNLDPETTANLDIFTRVA
                     SSSSIIQ"
     gene            complement(3780..4625)
                     /locus_tag="PKH60_00440"
     CDS             complement(3780..4625)
                     /locus_tag="PKH60_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNQ16551.1"
                     /translation="MNALQRISLFGWQWVAESKIQVINILKKHLDNDSADLLSISTAN
                     PEQVMLAQQAKQFFQTVKNEFDLVLPDGEGIVLASRLLRNRTEEARILERLAGVEIVS
                     KLISRSSLKHGSILVIGGKGYQNSEIKIDSARFSFREVTNSNELTVDSSNKYLFWLPA
                     YEDAKHPTMREQELVESVIKKLKPKLVFVALGAPQQEFWVVKHKKLLKDSGVKIAMVV
                     GGAFDMLTGKVPRAPRLMRQLKLEWFWRLIQEPSRWRRQLALINYILYLAKLLVRTDI
                     NKRAQ"
     gene            4709..5581
                     /locus_tag="PKH60_00445"
     CDS             4709..5581
                     /locus_tag="PKH60_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNQ16552.1"
                     /translation="MSQPSFSYHFESAYTLEEVWALLTDLNFWHQSWQNLVQRLTLSE
                     PIRLGSRGVITFREGESWELLITDFRPARSITFQFRSEKGRISVIYQLLQQEKLVISC
                     TVKLEHYLLDSQNIVSTLINPIITKHLNQLRLIISQPKNTEKNESATPIALHNLDVQA
                     GFRLWQLANLWQSKIRVVLKPFQLTPTQWLLLYALVRLEKHPEPITPTMLAEILEINT
                     MLVSDVITALVKKHLVHKIKILPDKRSFVISATAEGRITAVEAHTAIIQTDKKFFSPE
                     PLASIYKLLIPQKH"
     gene            complement(5578..6354)
                     /locus_tag="PKH60_00450"
     CDS             complement(5578..6354)
                     /locus_tag="PKH60_00450"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNQ16553.1"
                     /translation="MKIKVLSLNTWMGGILWQPCYDFVTAQQADIILLQEIYSSVDDK
                     LDPRFRSKLLFESELSEFQSFFSAFICDLREKEGPIDNGNMILSRWPMVETETIFFDE
                     PYASYDHDNTVDFSQWAAGAQRVIVGLPGDKKLQIANLHGPVWYEGAEPTERRMNMIK
                     SLNTVCSQTLPTIIAGDSNATPDNPCWQKLDPRYTSVFNNQLKTTFNMRRKTNPGYAT
                     AAVDVFLISTGIKVMRAQCLDLDISDHLPIVVEIEIDNLA"
     gene            complement(6357..7262)
                     /locus_tag="PKH60_00455"
     CDS             complement(6357..7262)
                     /locus_tag="PKH60_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNQ16554.1"
                     /translation="MLIDTHCHLNFKVFRSQLPVVLERAQKVGVEQMVVVGSQLKNSK
                     RAVEQAQAHEAVFAAVGVHPHHVWDYLEKAKSQAKIAGQSLETVMNEIIELVTAELID
                     LVRQPKVVAVGEIGLDYHQFEQTQYDGKVITPDYQLWQERFFLLQAKIASNANRAIII
                     HNREAVDDLLSLLTKHAYLFTNNQVVYHCCEPDQRMLGYAVDHQFYVGVDGDVTFDLA
                     KQDFIKQVPLERLVLETDAPYITPEPDKSYFAEHKNELSYKDRVCEPRHVAVIANKVA
                     EIKNLPVDEVANQTTKNAHQLFSLS"
     gene            complement(7298..9451)
                     /locus_tag="PKH60_00460"
     CDS             complement(7298..9451)
                     /locus_tag="PKH60_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ16555.1"
                     /translation="MASRNLKEQEFFPGLTVAELTEDPTLVLPGGIDLSQFDRNERLL
                     IHAALEQSLQIEEDTLRGLEVSIDPSQNYIIFGLSDLHFGSNATDLAALFALQRVILK
                     IPNARIILLGDEIDGRGKYSTNDIRNTKVKRQIDLFRAWLKPLYDAGKILAAVGEYNG
                     HNGWVVDTNAVGWEFIFGETMPVPILGNGETLQIIDESSEDADLLVSISMAHSSGKRS
                     ATDPVHGLRSLLNREGTDAAIAGHTHTFGLEQEAKWGRNGQILKATLVNAGAFKGSSK
                     DLGVPRDTFGQRLALGEVSPGGSGIIFSADLVDPRTSVKQIEGYPYANRHEGVFLLAA
                     LNLLNDLEAQQLTDEILQQARIRSKNPNPRATKYRLKHIIPQSQSEIGQSPAEKENWE
                     QKQIEYIARLTKEGSDIPEDRKQQSEWFHDPYEPNYQNLVARFDSDGLPFGIHLLSNL
                     GLGAASFRKDQFEQYLSQLNNPWDLLLWLRGVIDKDAGKLRDRRDHLDTAVGWMDQNS
                     SRNLAFLIDGSLRAAAWKSTAKGFSLKGGVTQADMLKYGPIAPASYISRASKVPLIPN
                     RGAIELRVGTNTDWRPYKIRALDRLGNSGSVFKPNFGLFRAYESFFEYPDLIVGGHMP
                     RSAFSKIYDKNNLSSGYPTFVSPGWWREVDSMGKGNVMSGGPPEYSVIVVPFDGGFIQ
                     YPTQGFHEYETLMKAVTLEIATLKNPKLKQQLRRG"
     gene            9563..11020
                     /locus_tag="PKH60_00465"
     CDS             9563..11020
                     /locus_tag="PKH60_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16556.1"
                     /translation="MLNDSMVGELRLQHPWFKYRSSNWEIVGGFLQLEWKFVTEPDLE
                     FSPTMLIPLPADDWQQRVPAQLIDQLVFHIGLVEMLSYWKATASPNIVVQAGYLNDAQ
                     LTWWHNFLLKSMGEYFYLNQINFTAPDFVIPVCTAPHQATKEQVNPVIQNEVDQRFLV
                     PIGGGKDSIVTMELLRQWRQHEAKPVKPDQLTLFAVNPTAATTAVIAQSGFPAIVVQR
                     NLDPKLLQLNQQGYLNGHTPFSALLSFVSLLTATIHGFSSTVLGNEQSANEGNAEYLG
                     QSINHQYSKSFEYETAFNQYVTTHIQSNYQVNLPNYFSLLRPFNELQIAQIFSQMAEA
                     YLPIFRSCNRGQKTNSWCCECAKCLFAYTILFPFLGEQQMVSIFGKNVFENADLLTTA
                     LDLIGKTKNKPFDCVGTYQESQAAFYLCYRWYVDRELEMPSMLMLLAETHLTNQVELA
                     EIAKNLLYQFNEEHCVPQVLGHYLSRFDLVTTNPK"
     gene            complement(11026..11547)
                     /locus_tag="PKH60_00470"
     CDS             complement(11026..11547)
                     /locus_tag="PKH60_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16557.1"
                     /translation="MFIFSERREYIGQIPGLYSEEEIQFIFSSSIRNNPEKMYELMIG
                     LHDALEFNKFVSMFELSLKISFAALISTELPTSFFMPGIFWPKSEGWFFTINEIAVIL
                     DLPTPEYPEDYLAIYSRCLTVNDDSSLISLYSHRIANTLRQLGLGIDAVEYAGFEYKP
                     LSREYRDLIASLF"
     gene            11604..13001
                     /gene="murD"
                     /locus_tag="PKH60_00475"
     CDS             11604..13001
                     /gene="murD"
                     /locus_tag="PKH60_00475"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNQ16558.1"
                     /translation="MRIVIVGLGREGWSSYQFLKNYLPKDTVFDLIDDGDPTSLSGQW
                     SSEMITHSEQYLATNSEPPSVAVLAPGIPPNHPLVVWLEANEVEITSNVQLFLDIVKP
                     NSDNKSVLLPYLPKLKQPVKTIGITGTKGKSTTTALLHHVLSQSGFDAHIAGNIGVPA
                     LDLITQLQNSSSEISIAVLELSSHQLSRLLTSPDIAVVLHVSADHLDYYQDFETYLEA
                     KATIARYQTAEDLLVYDADSPTASRIAQLSPAQKHTFSTSNPEADCYLKQGATTLKQG
                     QAVNLTNLPLVGEHNQRNALVSVLISLHLGVSLDELLSALHSFRGLPHRLQLVHEANG
                     VRFYNDSLATNPQAGSAAVRAFPNQNVILIAGGYDRGIDYAPFAEAIAASTVTHAILL
                     PTTGSKIAEGLKKINHSIKVEQVPDLTAAVTRAKQLAQPEDVVLMSPASASFNQFKDY
                     AERGETFTRLVIASA"
     gene            complement(12993..14252)
                     /locus_tag="PKH60_00480"
     CDS             complement(12993..14252)
                     /locus_tag="PKH60_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNQ16559.1"
                     /translation="MSESYFTLERVAQILQVSYLTVYRWVRAGKLPASKIGRQYRISQ
                     SNFAAFMDQDKPRSIGSVSQVLHRFIGSGLMDYSYIEGRHFAIDLTLGINPLGAPFDV
                     RKYLGKVKIDYSQYSSVTAVQLRNKLAQFHQIEPSQILVGAGASELLHLVFLTFLNPG
                     EKTLIPETTFPAFEMLALLVHANVILIPLTQAFDIDLDSISKHISAETKLVVLCNPNN
                     PTGRGLDYQSTLELITNHPQTKFLIDEANIDFGATSFVNDFGRLSNVIIARSFSKGFG
                     LAGLRVGYLISHPDAIYALQRRQTPFMTNVVGQVVAARALDFPKFLEKSRQYCQVQRL
                     WLEIELKKLGFSFVASESNYLLVDVTSVDLRSKELIAKLNHYGANAIDGRDFRGLGNR
                     YIRLSPRGTKTNQKFIAILKKVAEKSG"
     gene            complement(14383..15126)
                     /locus_tag="PKH60_00485"
     CDS             complement(14383..15126)
                     /locus_tag="PKH60_00485"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNQ16560.1"
                     /translation="MKLLTVNVEGHKHVDLICNLLKTEQPDVICLQEIFEADLPLFQK
                     ALQHEDQFQVLFSPNANVLKQNQYGIAPMGKWGIALIVNRAFIQDSMHDFHRLFYVGE
                     ERVVPEFEGPYSVRRSLLVAQLTVDGQSFAVATTHFTWTPNGQASPQQHSDLKQLKLH
                     LSNYNRFILCGDFNAPRGGEIYSQIAEGLIDHVPRDVLTTLDHTFHYAGMLNLVVDVV
                     LSTLDIGSISTRVIDGVSDHKAIVVEFAV"
     gene            complement(15139..16164)
                     /locus_tag="PKH60_00490"
     CDS             complement(15139..16164)
                     /locus_tag="PKH60_00490"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNQ16561.1"
                     /translation="MKFFRTLFRQFAFDLGSLNTRIWVEGKGVVINEPTCLAIAPSGE
                     VLATGKEAVEIAGRQNQDVQIIWPVEAGVIMDESALIALLKVWLRPYFKPTLLLQPTV
                     LVSTTAESTQANRSVIRSVFEKLGAVEVITIAQPLAAAIGSGVHTADATGSFYLHLGD
                     GVVEAGVVSLGSIMVTRQSLLAGHYLAEQMSLLLNNDHNLIVDSMVVEKIKREVFSLL
                     PDQNRMQVVMGKSSQTLRPIKQEVAAKHLVQLAQQLTGEYTTLLFHVLEEAAPALLVD
                     SLDKGLLLSGGLAQLHGLDRALSTEFKMPVSVVDNPDLVVIEGMSKVLEHLDDFRQSL
                     SYHAELV"
     gene            16186..17532
                     /locus_tag="PKH60_00495"
     CDS             16186..17532
                     /locus_tag="PKH60_00495"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNQ16562.1"
                     /translation="MHVPRNKGLLFTVFSALVIVVGTYMAIRYAKGGFRVTDSGISQE
                     TGLLSVSSLPTGAEVYINEKLVTATSDTLYLDPDQYEVAIRKDGYTEWKKIITLEKEV
                     VSQANALLFPSAPSLTPLTFTGIENLSPSPDGQKILFYSASNSAQKKNGLYVLELSDN
                     SLSLQRGARQISQDSSTIDLATAKFIWSPDSTQIIVFGSNREVLLDVSRLNDLDALSD
                     ISFRRKQLLSDWEDEMALREQQFLGKFPPAIIKIATSSATNVFFSPDKKRLLYTATES
                     ATLDPNLIPALPGANSQPEERQLQAGGIYIYDREEDKNFRVGTQQDKTVFPAKSLLST
                     LFEQNQATPNTIATTSARRSLEASSSSELADLYRNYHSSLFADTYQWFPDSKHIFSAE
                     DSRIVIKEYDNTNAVTVYSGPFFQNFIYPWPDGSRLLIATHFNPETPNNLYAIELR"
     gene            17685..17758
                     /locus_tag="PKH60_00500"
     tRNA            17685..17758
                     /locus_tag="PKH60_00500"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17719..17721,aa:Pro,seq:cgg)
     gene            complement(17763..19922)
                     /locus_tag="PKH60_00505"
     CDS             complement(17763..19922)
                     /locus_tag="PKH60_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNQ16563.1"
                     /translation="MTDQNTSHKTVSATVERVFTPIRVPTKPLRYRLKLLENSRKKLQ
                     QLTLVSLEVGQKGLAFFKRFVHHPLKSIKLIGEAILFQVRRHWFGQPGELSWSYLILI
                     GRKNIMRSFSRTAVTVGAIAAGSAAIVVLVGFAYGLESIVTKRLVQPNSLRLADVQST
                     STAYQIDKPAIEQLSQIAGVENVSMSVNLAGSVKYGDAQTEVVVVGARNDFLDASHIE
                     LVAGERFSEEAEMAYSGEVLDLSRLATASAGLVSGVSIEVEPIAGDRVSDQPVNFRIS
                     DDVYVPLRAEPNTKSEIVGYVRGSILERHQGYKVWGGAYDTVGTAGRAHQLSTGTWLG
                     EWLKIEQGLPLYMEQAPTVFAPIVEAEGKQKLGVGYLTQNSVGLLTMQEALAEKALEV
                     LHLEAQEREDREQVLGDNTEDNVVGVVLDKTATSSASEQQLLESMIIQSQPQSGSASA
                     TLAVASVAKRGGKEILVSTGFLNALKLTPDSLIGKNLSVQYILSGGLVAGMKGKVTSQ
                     ATDYTIVGVFDLPEQSMVFAPLSDLESMGVSKYSLAKVLADNSEVLEGVRERIGALGF
                     RTRSIADTLLQVNKLFSIMRFLLGSFGMIAFIVALFGMFNTLTVSLLERTREIGVMKT
                     LGTTDRDIRRLLIVESSLIGAAGGVLGVLFGVVLGNSLNWISMLSRSDKTLSLFQFPI
                     LFLLFVFLLAALVGMLTGLYPARRAHKISALNALRYE"
     gene            complement(19915..20718)
                     /locus_tag="PKH60_00510"
     CDS             complement(19915..20718)
                     /locus_tag="PKH60_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ16564.1"
                     /translation="MPKKKIITHQKRAKPELPLVAVDVVALEKKLPPKAARSHHVVIK
                     VDKVGKSFPVGESVVPVLHDVSLNLYSGEFVIISGPSGSGKSTLLHSILGLEPPSAGS
                     VIMRDVDLYKDLDDQGRTRFRREKVGVVFQQSNWIKSLSVIENVSYPLWLAGESATSA
                     RQTALNWLEQVELAEWADHHPSELSGGQQQRVALARAMVTDPGIIVADEPTGNLDSEA
                     SEDMIALLAKLNREQSKLILMVTHNLSLLPIATRRTVLKDGRIIYDQHD"
     gene            complement(20718..>24537)
                     /locus_tag="PKH60_00515"
     CDS             complement(20718..>24537)
                     /locus_tag="PKH60_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16565.1"
                     /translation="AYVYDGTTGNVGGTVASTSAKLVFGGNVVTPAAYTDLGGGWWRL
                     TYSAATTDASLLYGVQALAGKTVYVDGVQLEAKTYATTYADGSLGSGYSWSGTANESA
                     SSRTVDNLRYVNSGSFSASRGSLSMWVKPNSSETNSKSIFTVDDWIFVVKQSAAYSVA
                     VRESDGGWASEPSFGSVVKDRWGLVTITWDGANNLKLYFNGVFVNTATWDGTMSSSTQ
                     LHIGGGWTREGDSTLSDLRIFNQALTPSEVSALYQQGLVTHSSGSEAVDRYSASGTYT
                     SPVIDLGANGAWGSVPWSASNILNGGGINYFTRTSMDNSLWSEWQAVTGSVDEGSWTG
                     GIASDPRRYFQWKADLSSNGDQSQSPVISAMSTTYVEDSTPPANPAQVALGYVNHASS
                     SATLTSNNWYNYSTPKFTWEPGVDDPAAGQSSSGIDGYHILLTTNQAATPSANLANPC
                     YRFAESDDRVFSVGTTPAGCSLSDADYYLRMQTKDNSGNVSEPVTLFTYRYDSTSPRS
                     PVSVSSTTVGYTSQNEFSFYWPAASDQGLNQSGLAYYEYKTGATEGAFSTWTKSNEPG
                     DPLVTLASNITAYQEGQNFFFVRTVDFAGNVSAEISNLGVSPFYYNESAPTAPVNVAI
                     SPVTSSDAQALENIFTVTWDKPASYSGELAKYYYCVNCTPSVETMTQTTSVQTADRTL
                     TNIALATQQGKNTFYLVAEDNNINTNTGRGNVNFEAYATAEFYASTIAPGAPTNLTIS
                     DASDRDAEVWRLTLAWKQAVTGGTPNFYAIHRSTDGTTFTKIGETTSTAYTDADLTQS
                     QEYSYKVFAIDNANSTSLSSNIVTLAPEGKYTTPPLAGGTPNVTVGSTVATITWSTGR
                     IAYGAVEYGKTTSYESSVAETVAVGNHSIKLTGLTPGTVYHYRVQALDDSILMGYDRE
                     IAYSSDYTFTTSALPTISNVEVTDLTLASAIINWSAVSLSSATIEYGESTAYGASVSV
                     GVGSSGTYSTKLSGLKDSVTYHFRIRAVDIDGTDITSDDYTFTTLTFPKVTAVVFKTD
                     QAQNATAINVAWSTNVPTTASVEYQAVKIDPSFASQSEINYSNSSVLQGLSQTELARL
                     PVVPLGSPQYLDRSTISNRHVVQITNLSDSSLYVFTIRGRDEYGNQAVSDPIRYVTGA
                     DTKPPSVENLVIETPIVGEGVESKAEIVLSFTTDEPAFSQVLWGAGTGSEYPQATQTS
                     SEATTDHMIVLRDLDPTSTYHLKIKTTDQTGNTSETEDTVVVTPTAQAAAFELILKNL
                     EGIFGFLGLQ"
     assembly_gap    24538..24637
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<24638..>27164)
                     /locus_tag="PKH60_00520"
     CDS             complement(<24638..>27164)
                     /locus_tag="PKH60_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16566.1"
                     /translation="GAKYVSGLVGVDNSSSSTGAIPASTYASHQGVFIEEGTTNLITN
                     PSFENASSATLNWGTNYFNYATASATFTPSMAKRNSAGPFAAGPMVQGKSDASYAWNN
                     GDSISWSQSNIAGSLTSTIDRSQGSIVFWITPEWNGNDGKTHYIFGQATDFALFKHSD
                     NRLVLYDYGWSYLFDISDWTAGSTYNIVIRWDQKEKIGNSNYNFIITVNDVEKFTRTT
                     SYVVGGGFYRLDPMDSIIEGLTIYRRPLFDGTYGTDVGNADEIAQIYNSGTGKDPTLV
                     TGSWDVVFALPTNASTGALTTGTGNAWSHPHSSNLLYTSTTNTGGFMMAGDAGVDGWL
                     ASPQQISGLKLWLDAGSIMGLSDGDQVSSMTDLSGSGFHATQTISANKPIYKTNQIDG
                     KPVIRFDGTNDYLSVGTIRATEGGVTSLVVSKRDSIGGTQWQRIVGSATHGDDSIAPG
                     WVITGASDGAGNPLVYTPSILRASHNSGRVISNLKIGVDSSLYGRYFVGDVAEILVYD
                     RELNSVEESAVRSYLTSKYDLTNFEVTALSSSEKIFSGGYKFTSNATNQGIYRSFTAT
                     NGGDYVLRALGHSDGTCNPQVKITRADGSTEISHLNGTTTSTRTDPDVYIFTWESPAA
                     EANQVQLINTASSGTCYWHQVEVFPNLITNPSLETGIGSAPYVPTGWVTNGSSGGSVE
                     SMDCHSGSACLNGSIEHNRFYGLNDISPALTTGKFFSWGHWSKMLNGSNIRLLEGTHI
                     QQHSNSAYQEYSISYPSWTASQTVQRVITSNPELYWFNQGGSTTSFLLDDAYAFALSD
                     VSLTVTPANQANSTEASGLRVDGADTLTQPITGLSADRGVIKFK"
BASE COUNT         7766 a         6435 c         5420 g         7443 t
ORIGIN      
        1 cagcggaatc cagctgccca gctctgaaat cacacaaatc ggcgcgccaa accactgggc
       61 aaatacatgg gatagcatta ctgataacgg acggatgtat cacagctcac ataaaggaga
      121 ttgtttggtg gctgtagcaa tttctgttgt tggtcgacct aactctgatt cacaacacgc
      181 tacggctata aaacgaaaaa gttgtcctga ccatacaccc tctcaagttg aaacctactg
      241 tcgcagtggc caagatccgg ttctgagttt tcatgcaaaa gctaaagcgg gactcgagac
      301 gggggcggcc gtccaaaaaa ttgttccaaa cggccgcccc gccgattggg aagcctgcgg
      361 ctactcagtc atcatggaat atcctccact cactcaagcc ccaaccatca gcaactcatc
      421 atccaaaggc acccagttac tagaatcagc ccttatctac gcttttcaac atggaatcac
      481 gaaagtttac gcctactcta gaccagtagg actacagcaa catcttgctt cgtctcttag
      541 ttgacacagt cgcttttgaa aaacttcaca agtgagtaaa ttaatgtggt tgcaccctct
      601 tgatcatcag tggcatctga gtaaaacgta gatacttcac ttccacttct cccaaacagt
      661 caactcttac acgatcagaa attttctttt cattcaagag cagattgaac gttgatctcg
      721 atatcaaaac cgtatcagaa ttctccaagt gtaagtaata agaacgatga tttttggaca
      781 ccgttcgttc cactttacaa ctcccactgt aaacgcgggg tgggtaatag atatcaagca
      841 ataccataca actttccatt atgatggcag ttgggagcaa cgaataaata gtaattacta
      901 agcacttttt ccatatgttt tcgattctgg attgattaat cttttttgcc gatgaagatg
      961 agcggttatt tatcaggaaa atagtgaatc ccagaaacat actaattgga gcgaaagtta
     1021 atggattgtt tatacaaatg atcacgataa ctatcgcgaa taaaagtgta gaaataactg
     1081 aaagtacaat gtttgaaata gtatttgttt tacccgactt tctgtttacg aaatttagta
     1141 actctgaaaa actggtttta cataaagcaa ctaaatgcaa aataagtaca agtaagctca
     1201 agatgagaaa aacagtaaat agttgtacgt aattatcttg actgactatt aagggcaaaa
     1261 cgaaataata gaaaataagc aaaatagatt ctgcaaagag catggtccaa attgttagta
     1321 cgcgcttcat cctgttaaat tctaccaccg accagactgc gacatcatct tacttcatct
     1381 cttggttaac ccgatcgctt aagaatagct tcatcagaga ttgatacggc atatcgtatt
     1441 cataatcaac attcaaaaaa ctaaaattgc tcaactctac gctttttccc gtccgtctgg
     1501 actacatcac tttcttgaga aaaactcctt atcaaaatag ctcaatttgg ctcttgacct
     1561 acttcaattc actctgctac tgtagagata gttaacaatt atttatgtat atattagata
     1621 tctttagaga ggtcatatga gcaccatgcc tatgtcaaac aacgtagtag atgagcgtcg
     1681 cactggtcgc gccgacgatg ccaacaaatt ggacatgcgc aacttaccag atttactcac
     1741 tgtgcgcgag gtggccgatt tactcagagt ctcccctctc accattaagc gctggggcaa
     1801 acgtggcaaa ttacccgcta ttcggatcaa ctcacgcgga gacagacgtt acaaaaaaga
     1861 agctgtattg tggctactgg gcatcactcc agaagacgcc gccgaagcga tggcgtaatt
     1921 aagttactct tgagcttcga aaataattgg accagtcgcc tttcgcttta ttcaacacca
     1981 ggcaaacatc aatatcgtaa ggattttgca ggtataccca aacgcttttt acatcaggca
     2041 gcaaaattcc ctcaggaaaa aggcgtgcta acttctcata cggtgtggca aaagccagat
     2101 ctatttgcac taggggtttg gaacagttgg cgaacgatac aatctcaaac ttcggttttc
     2161 taatttggat ataatcaact ggttttttac cctcaacctc agcttgggta cccggcaaaa
     2221 taaaatccca gggttctcca ctaggaaacg tgtgatacaa acattttgtt ttcaactgat
     2281 ctcgtaaggc agcaatcttg ggatggactc ctgccttgaa ccacggcaaa ttaaatccaa
     2341 gatgatcaac attttgcagt gcagctgctt gtattcttgc tacgaagtcg accatggcta
     2401 ttggagtgta agcatattga cttttctttt ccgttccaga gctattttca actacgactt
     2461 catatacttc ttcgtcactc atagtaaatc tgaccaacgg ttgctcaaca acatcttctt
     2521 tgagtgaaat cagaggatag taacttcttt tggtggtgtg aaacaacaat gattgtggtc
     2581 gcaactctcc catatctctc aagaaggtag ttaaatcatt gaaaaatgaa cttgaatttt
     2641 ctgggagtac tatatcaatc gtcgtcaact gggacatacg ctaagtataa accatgcttg
     2701 caacccgtct ctggtaattc tcgatatatt tttcaaaccc atggtttttg aatcagttga
     2761 tgcaaacttc gctggcatat ttgccggcta aagtgctcag ttgaaaaaat ttcacaacat
     2821 cactagtggc ccgtagttct ttggggtcgg caactttcac agataaatca tagcagatca
     2881 taaacatgct aaaatgaatt catggatcaa acatcaaatc aaactttctc cgaaccgatg
     2941 gattcattga gtgaaacaca accccagaaa ccctataaac ttattggctt ggcagttgcg
     3001 ataatcatta tcgcagcatt tttagtattt ctcggctcaa cacgcattac aacaaaaaca
     3061 actcagctgc aacaaccact tgagtctcaa caacccactg agactgaaac ttattcgatt
     3121 gatactcaag agccagaaca acagaaagta atagatcccg atacagtaga atggaaaact
     3181 tttgtaagtg agctgggctt ctctttcgac tatccatcgc attggggaac tccaacagaa
     3241 aaagtactcg atgctgttga agccttgcag ggtgatactg gcaaaacgta tcagctcacc
     3301 tttagtaata ctcaagaagt atatgcgtct ggtagatcgg ctgacttttc ggcaggcaga
     3361 ggtggtcgtc aagaagactt tggcgttaat tccaacggca aaactagcat tgaagcatac
     3421 ctagatattg tggatccggg ttgttttacc atggggaacc agaatagttt tcgtgcgctt
     3481 attgactttt cactagtaga taaacccgtc ggaggcgtac ggctatttct cccgattgta
     3541 tctcagagca ttctcgaaaa aattaaagag gaatttagta tccccagtga gacaatcgat
     3601 tgcttttcag atgttgaagc acagaatcag ctgatcgaga aaggtaaaga aattcgaagt
     3661 atgcttgtag ctaatactaa tctagatcct gaaactactg caaatcttga tattttcact
     3721 cgagtcgcat cctcaagttc aattatccag tagcatatac caccgtcgtt ttaacttgtt
     3781 tattgggctc gtttgttaat gtcagtacgg actaatagct tggccaggta caagatatag
     3841 tttatcagcg ccagctgacg gcgccaacga gaaggttctt gaattaaccg ccaaaaccat
     3901 tcaagcttga gctggcgcat gagccttggt gcgcgtggaa ctttgccggt gagcatatca
     3961 aaggcgcccc ccaccaccat tgcgatcttg actccagaat ctttcagcaa ctttttgtgc
     4021 ttcaccaccc aaaactcttg ctggggggcg cccagagcta caaaaaccag ctttggcttc
     4081 aatttcttga tcacgctctc aactagctct tgttctctca ttgttggatg cttagcgtcc
     4141 tcataggctg gcaaccaaaa cagatatttg ttgctcgaat caacagtcaa ctcattcgag
     4201 ttcgtcactt ctcgaaaaga aaatctagcc gaatctattt tgatctcgga attttgatac
     4261 ccctttccac caatcactag tatcgaacca tgcttcagac tcgatcgaga tataagtttt
     4321 gagacaattt cgactccagc cagacgctcc agaatccttg cctcttccgt tcgattcctc
     4381 agcaatcgac tagccaaaac aatcccctcc ccgtctggca gcacgaggtc aaattcattt
     4441 ttgactgttt gaaaaaactg cttggcctgc tgtgccagca ttacttgctc aggattggcc
     4501 gttgaaatgc tcagtaaatc agcgctgtca ttatctaggt gcttcttcaa gatgttgatc
     4561 acttgtattt tcgattctgc cacccattgc caaccaaaga gactgattct ttgtagtgcg
     4621 ttcatatagc tattgtaccg caccgacgac tgattctaga caaataacca caatcggata
     4681 taataagcgt gcttagtacc aggaagccat gtctcaaccc tctttttcct accactttga
     4741 gtcagcatat acgcttgaag aagtttgggc actcctcacc gatctaaact tctggcatca
     4801 aagttggcaa aacctcgttc agcgactaac gttgtctgag ccaattcgcc tgggaagccg
     4861 gggggtgatt acgtttcgcg agggcgaaag ttgggaacta ttgattaccg actttcgccc
     4921 cgccagatca ataacctttc agtttcgttc ggaaaaaggc agaatttcag tgatctatca
     4981 gctcttgcaa caagaaaaat tggttatctc ctgcacagtc aagcttgagc attatctttt
     5041 agattcacag aatatagtaa gcacgcttat aaaccccatt attaccaaac atctcaatca
     5101 attacgtttg attatttctc agccaaaaaa tacggaaaaa aatgaatcag ccactcccat
     5161 cgctcttcac aatcttgacg ttcaagctgg ttttcgtctc tggcaacttg ctaatctctg
     5221 gcaaagtaag attcgagtgg tcttaaaacc atttcaactc acacccacac aatggttgct
     5281 tctgtatgcc ctagttcggc ttgaaaaaca tcctgaaccc atcacgccta ctatgctcgc
     5341 agaaatcctg gaaattaaca cgatgttggt ttctgatgtc attaccgctt tagtgaaaaa
     5401 acacttagta cataaaatca aaatattgcc agacaaacgc tctttcgtta tctccgccac
     5461 tgccgagggt agaatcactg cagttgaagc ccacaccgcc attattcaaa ccgataaaaa
     5521 gtttttctcg ccagaaccac ttgcttcaat ctacaaactc ctcattccac agaaacatta
     5581 agcaagattg tcaatttcga tctcgacaac tatgggcaag tgatctgata tatctaaatc
     5641 caaacattgt gctcgcatca cctttatgcc tgtagaaatc agaaatacat ctactgccgc
     5701 agttgcataa ccaggattag ttttcctacg catattgaag gtagtcttca gctgattgtt
     5761 gaatacagag gtataacgtg gatctaattt ttgccagcag ggattatctg gtgtggcatt
     5821 gctatcgccc gcaattattg taggcagtgt ttgactacag acggtgttga gggactttat
     5881 catgttcatt ctccgctctg tcggctctgc tccctcgtac caaaccggac catggagatt
     5941 ggcaatttgt aactttttgt ccccaggtaa accgactatg actcgctgag ctcctgccgc
     6001 ccactgagaa aaatcaactg tgttgtcgtg atcatagctg gcgtatggct catcaaaaaa
     6061 tatggtctct gtttcaacca tcggccaacg actcaggatc atattcccgt tatcgattgg
     6121 gccctctttt tctctcaaat cacagataaa ggcagaaaag aagctctgaa attctgaaag
     6181 ttctgattca aaaagtagtt ttgaacgaaa cctaggatca agtttgtcgt caacactaga
     6241 gtagatttct tgcaacaaaa taatatcagc ttgttgggct gtcacaaaat cataacaagg
     6301 ctgccaaagg attcctccca tccaagtatt cagtgatagt acttttatct tcatgactag
     6361 ctgagtgaaa agagctgatg ggcgttcttt gttgtttggt tggctacttc atcaacgggc
     6421 aaatttttga tttcggcaac tttgttagcg atcaccgcta cgtgacgcgg ttcgcaaact
     6481 ctatccttgt acgaaagctc atttttatgt tcggcaaaat atgacttatc aggctctgga
     6541 gtgatgtatg gtgcatcagt ttcgagcacg agtctttcaa ggggtacttg tttgataaag
     6601 tcctgtttgg ctaaatcaaa ggtcacgtcg ccatcaacac caacataaaa ctgatgatct
     6661 acagcgtaac cgagcattcg ttggtcgggt tcacagcagt gatacacaac ttggttattt
     6721 gtaaacagat aagcgtgctt agtaagtagc gaaagcaagt cgtcaacagc ttcgcggttg
     6781 tggatgataa ttgctctatt tgcattgctg gcgatttttg cttgtaaaag gaaaaatcgc
     6841 tcctgccata actgataatc tggcgtaatc accttaccgt catactgagt ttgctcaaat
     6901 tggtggtaat ccaagccaat ttcgcccacg gcaaccacct ttggttgccg aacaagatca
     6961 attaactccg cggtaaccaa ttctatgatt tcattcatga cggtttccag actttgaccg
     7021 gcgatttttg cctgggattt ggctttttct aagtagtccc aaacgtggtg tggatgcacg
     7081 cccaccgccg caaaaaccgc ctcatgagcc tgcgcctgct ctacggcccg cttggaattt
     7141 ttcagctgcg aaccaaccac caccatctgc tccactccaa ctttctgcgc tcgctccagc
     7201 accaccggca actgcgaacg aaagacttta aaattgagat ggcagtgagt gtcgatgagc
     7261 attttgaata gtataccctt tgcaacaaac tatgcctcta acctctgcgc aactgttgtt
     7321 tcaattttgg attttttagg gtggcaatct caagtgtcac tgccttcatc agtgtctcat
     7381 actcatggaa accctgagtg ggatactgga tgaaccctcc atcaaatggc actacaatta
     7441 ctgaatattc tggcggacca ccagacataa cattgccctt acccatagag tcaacttctc
     7501 tccaccatcc gggagaaaca aatgttggat acccagagct aaggttgttt ttgtcgtata
     7561 tcttagagaa agctgaacga ggcatgtgcc caccaactat tagatcggga tattcaaaga
     7621 aagattcata tgccctaaaa agtccaaagt ttggtttaaa aaccgagcca ctattaccaa
     7681 gtctatcaag tgcacgaatt ttgtacgggc gccaatctgt gtttgtacca actctcaact
     7741 caattgcccc ccgattagga attaaaggta ctttagaagc cctactaatg tatgaagccg
     7801 gggcaattgg gccatacttc aacatatccg cttgagttac tccccccttt aaagaaaatc
     7861 cctttgcggt actcttccat gccgcagctc ttaacgatcc atctatcaag aaagctagat
     7921 ttcttgagct attttgatcc atccacccaa ctgcagtatc taagtgatct cttcgatccc
     7981 tgagtttacc tgcatcctta tcgatcacac ctcgtaacca cagaagtaaa tcccagggat
     8041 tattaagttg agataaatac tgctcaaatt ggtctttacg aaagctcgct gctcccaaac
     8101 ccagattaga aagcaaatga ataccaaatg gtaagccatc tgaatcaaat ctggctacta
     8161 gattctgata atttggttcg tatggatcat gaaaccattc gctctgttgc ttgcgatctt
     8221 cagggatatc agatccttct tttgttaacc tggcaatgta ctcaatttgc ttctgttccc
     8281 aattttcttt ttctgcagga gattgaccaa tttcgctttg agactgagga atgatgtgtt
     8341 tcaatcgata cttcgtcgct cgagggtttg gatttttaga tctaatgcga gcctgttgca
     8401 gtatttcatc cgtcaactgt tgagcttcta aatcgttcag caaatttaac gcagctaaca
     8461 agaacactcc ctcatgtcga ttagcataag gatacccctc aatctgtttt accgatgttc
     8521 gcggatccac taaatctgcg ctgaaaatga tgccagaacc acctggagaa acctctccaa
     8581 gtgccagtct ttgcccaaac gtatctcttg gcacacccaa atctttagaa gaacccttaa
     8641 aggcacccgc attgaccaaa gtagctttga gaatttgacc gttccgtccc cactttgctt
     8701 cttgttccaa accaaaggtg tgggtatgac ccgcaatcgc agcatcagtt ccttctcgat
     8761 tgagtagaga tcgaagtcca tgaactgggt cggttgcact tctttttcct gaggaatgag
     8821 ccatcgaaat tgacaccaat aaatcagcgt cctcgctcga ttcatcgata atttgcaggg
     8881 tctctccatt tccaaggatt ggcaccggca ttgtttctcc aaaaataaat tcccaaccca
     8941 cagcatttgt atctactacc cagccgttgt ggccgttgta ttcacctaca gcagcaagta
     9001 tttttccggc atcgtacagc ggtttgagcc atgctctaaa gaggtctatc tgcctcttca
     9061 cttttgtatt tcttatatca tttgtggaat actttcccct accgtctatt tcatcaccta
     9121 ataatataat tcgagcattt gggatcttaa gaatcactct ctgcaatgca aataaagccg
     9181 ctagatctgt tgcgttgctg ccaaagtgta ggtcgcttaa accaaaaata atgtagtttt
     9241 gactaggatc gatcgaaact tccaaaccac gaagtgtatc ttcttcgatc tgcaaactct
     9301 gttcgagtgc agcgtgaatc agcaggcgct catttctgtc gaattgagat agatcaatcc
     9361 ctcccggtaa taccagcgtg ggatcttcag taagttcagc tacagttaaa cctggaaaaa
     9421 attcctgttc ttttaaattt cgtgatgcca taactccgtt ctaaagcaag caacaaacat
     9481 acgtgaaaat aacagagcca aaccactacg tcaatgagta gatcataccc aaccatcgtg
     9541 aaataccgta taataaacaa taatgctaaa cgactcgatg gtcggtgaat tgcgcttgca
     9601 acatccgtgg tttaaatatc ggtcctccaa ttgggaaatt gttggtggtt ttttgcaact
     9661 ggaatggaaa tttgtaacag aacccgattt ggaattttca ccaacgatgc ttattccatt
     9721 gccagcagac gattggcaac aaagggttcc ggctcaatta attgatcaac tagtttttca
     9781 tattggctta gtagaaatgc tgtcttactg gaaagccact gcttcaccca acatagtcgt
     9841 acaagctggc tatctaaacg acgcacaact gacttggtgg cacaattttc ttttgaaaag
     9901 catgggggag tacttttacc tcaatcagat caactttact gcacccgatt ttgtgattcc
     9961 ggtatgtacg gcgccacatc aagcgacaaa ggagcaagta aatcctgtga ttcaaaatga
    10021 agtagatcaa cgcttcctag tgcccatagg tggtgggaaa gattcaattg tgacaatgga
    10081 attgctgaga cagtggcggc agcacgaagc caaaccagtc aagccagacc aactgacact
    10141 cttcgcggta aacccaactg ccgccaccac agcggtaata gctcaaagtg gttttcccgc
    10201 catagtcgtc caacgcaacc tcgatcccaa acttttacaa ctcaatcaac aaggatacct
    10261 caatggtcac acacctttct cggcactact cagctttgtg agcttgctca cagccacaat
    10321 ccatggtttt tctagtacgg tcttaggtaa tgagcagagt gccaatgaag gcaacgctga
    10381 atacttgggt cagagcatca accatcagta ctccaaatca tttgagtacg agacggcctt
    10441 caaccaatac gtgaccaccc acattcaatc aaactaccaa gtcaatttgc caaactattt
    10501 ttctctcctc cgccctttta acgaactaca aatcgctcaa atcttctctc aaatggcaga
    10561 agcatatttg cctattttcc gcagttgtaa tcggggacaa aaaacaaatt cttggtgttg
    10621 cgagtgtgcc aaatgcctat ttgcctacac aatattattt ccattcctag gcgagcaaca
    10681 aatggtctca atttttggca agaatgtatt tgaaaatgca gacttactca caactgcgct
    10741 cgatttgatc ggcaaaacca agaacaaacc atttgattgc gtgggcacat atcaagaatc
    10801 tcaggcggcg ttctatctat gttaccgctg gtacgtagat cgagaattag aaatgccaag
    10861 tatgcttatg ttgttggcag aaacacatct gactaaccaa gtcgaactcg cagaaatcgc
    10921 aaaaaatctc ctctatcagt ttaacgaaga acattgtgtg cctcaagtgc tcggacacta
    10981 tttgagtcga ttcgatctcg tcaccacaaa cccgaagtaa cattgttaaa ataaacttgc
    11041 aataaggtct ctatactctc tggaaagcgg tttatattcg aagccggcgt actccacagc
    11101 atctatgcca aggcctaatt gtctgagtgt gtttgcaatt ctatgagaat acaaactgat
    11161 caatgacgaa tcatcattaa ctgttaagca tcgcgagtat attgctaaat aatcttcggg
    11221 atactctgga gttggtaaat ctaatatgac agcaatttca ttaatggtga aaaaccaacc
    11281 ttctgacttt ggccaaaata ttccaggcat aaaaaagcta gttggtaatt ctgttgatat
    11341 caacgctgca aaacttatct tgagagagag ctcaaacatg ctgacaaact tgttaaattc
    11401 tagagcgtca tgtaatccaa tcattaattc atacattttc tcaggattat tcctaattga
    11461 agacgaaaaa ataaactgaa tttcttcctc agagtacaaa cctggtattt gacctatgta
    11521 ttctcttctc tcgctaaata taaacatata attcatagta tatctcatca gtcttcattt
    11581 acacctcctg ctacaatact cttatgcgaa tagtgattgt tggtcttggc agggaaggtt
    11641 ggtcgtcata ccaattttta aaaaattatc taccaaagga tacggttttt gatctaatcg
    11701 atgatggtga tccaacctcg ctgtctggac aatggtcatc agaaatgata actcattcag
    11761 agcagtacct ggcaactaat tcagaaccac cttcggttgc agttctggct ccgggcattc
    11821 cccctaatca cccacttgta gtgtggctag aagcaaacga agtggaaatt accagcaatg
    11881 tacagttatt tttggatata gtgaaaccga attcagataa caaatcggtg cttttgccat
    11941 acttgcccaa gctcaaacaa ccagttaaaa ctattggaat cactggcact aagggcaaaa
    12001 gcaccacaac cgccctgctc caccacgtac tcagccaatc tggatttgac gcccacattg
    12061 caggtaacat tggagttccg gcgcttgatt tgattactca attacaaaat tcatccagtg
    12121 aaatctcaat tgctgtgctt gagctatcga gtcatcaact ctctcggctt ctaacgagcc
    12181 cagatatagc tgtagtactt catgtttctg ccgatcacct tgactactat caagacttcg
    12241 aaacatatct ggaggcaaag gctaccattg ctcggtacca aactgcagaa gacctccttg
    12301 tctacgacgc agatagccca actgcatcac gcatcgctca gttaagtcca gcccaaaaac
    12361 atactttttc taccagcaat cctgaggcgg attgctatct caaacaaggt gcaaccacac
    12421 tcaagcaagg tcaggcagtc aatctaacca acttgccctt agtaggtgaa cataaccaaa
    12481 gaaatgcatt agtaagcgtg cttattagtt tacaccttgg tgtcagtcta gacgagctac
    12541 tctcagctct gcactcattt cgaggcttgc ctcatcgcct acaacttgtc cacgaagcaa
    12601 atggtgtgcg attttataac gactcgctag ccactaaccc gcaagctggt tcggcggctg
    12661 tgagagcctt tcccaatcaa aatgtcattt taattgcagg tggatacgat cgaggaatcg
    12721 actacgctcc gtttgccgaa gcgattgcgg caagcactgt cacgcatgcg attttacttc
    12781 caactacggg tagtaaaatc gccgaaggtc tcaagaaaat caatcactca atcaaggttg
    12841 aacaagtacc agaccttaca gcggccgtca cccgagccaa gcaactggct caacctgagg
    12901 atgtggtact gatgagccct gccagtgcca gctttaacca gtttaaagat tatgctgaac
    12961 gtggagaaac atttacccga ttagtaatcg cctcagcctg acttctcagc cacctttttt
    13021 agaatagcaa taaatttttg attggttttt gtacctcttg gagaaagtcg gatgtatcga
    13081 tttcccaacc cacggaaatc tcgtccatct attgcgtttg caccatagtg gttgagcttg
    13141 gcaatcagct cctttgagcg caagtcgacc gaagtaacat cgactaacag atagtttgac
    13201 tccgatgcaa caaaagaaaa accaagtttt ttcagctcta tctccaacca caaacgttga
    13261 acttgacaat actggcgtga tttctccaaa aatttgggga agtctagtgc tcgcgcagcc
    13321 acgacttgcc ccactacatt agtcataaac ggtgtctgac gtcgctgaag tgcgtaaatc
    13381 gcatctgggt gactaataag ataacccact ctgagcccag ctaaaccaaa tccttttgaa
    13441 aatgatcgcg cgattatcac attgcttagt cgaccaaaat cattaacaaa tgaagttgcg
    13501 ccaaaatcga tatttgcttc gtcgattaaa aacttagttt gtgggtgatt agtaattaat
    13561 tctagagtac tctgataatc caaacctctt cctgtggggt tatttgggtt acacaaaact
    13621 acaagttttg tttctgcaga aatgtgtttc gagattgaat ctaaatctat gtcaaacgct
    13681 tgagttaacg gtatcaagat tacattggca tgaaccagta gcgctaacat ttcaaaagca
    13741 ggaaaagtag tttcgggaat caacgttttt tctccaggat tgagaaaagt taaaaatacc
    13801 aagtgtagta attcacttgc tccagcccca accaaaattt gacttggttc gatttggtga
    13861 aattgggcca gtttgtttct caactgaaca gcagtaacac tggaatactg agagtaatca
    13921 atcttcacct tacccaaata ttttcttaca tcaaatggtg caccgagggg attaattccc
    13981 agcgttaggt caatggcaaa atgccttccc tcaatatatg agtaatccat caagccactc
    14041 ccaataaaac gatgaagaac ttgagatact gaaccaatcg atcggggttt atcttgatcc
    14101 ataaaggcag caaaatttga ttgagatatt cggtattgtc gcccaatctt gctcgcgggc
    14161 agcttgcccg ctcgcaccca tcgatacaca gtcaagtacg aaacttgtaa tatctgagcc
    14221 acccgctcga gtgtaaaata gctttctgac atactttaga tttatcaata tttaacaata
    14281 cttatcaaag tataacatag aaatacaacg ccctagtaac tccgatctga aaaaaagata
    14341 ctaacagtgg ctggcaacct agtgggataa acaggacgag atctaaacgg caaactccac
    14401 cacaatcgct ttgtgatcac taactccatc aataacccta gtgctaattg aaccgatatc
    14461 tagtgtactc aaaactacat ccacgaccaa attcaacata ccagcataat gaaacgtatg
    14521 atcgagtgtt gtgagcacat ctcgcggaac gtgatctatc aaaccctctg caatctgaga
    14581 gtagatttca ccccctcgag gtgcattgaa atcaccacag agtataaatc ggttgtagtt
    14641 actgaggtga agtttcagtt gctttagatc gctgtgctgc tgggggctcg cctgaccatt
    14701 tggcgtccaa gtaaaatggg tagttgctac agcaaagctc tgaccatcaa cagtgagctg
    14761 cgccacgagt aagcttcttc gcaccgaata cggtccttca aattctggca caactcgttc
    14821 ctcaccaaca taaaataatc ggtggaaatc atgcatacta tcctgaataa atgctcgatt
    14881 aacaataagg gcaatacccc atttgcccat aggggctata ccatattggt tctgtttcaa
    14941 aacgttggca ttcggtgaaa acaacacttg aaattgatcc tcatgctgta atgctttttg
    15001 aaataaaggc agatcagctt caaaaatttc ctgtaaacag atcacatctg gttgttccgt
    15061 tttaagcaga ttacagatca gatccacgtg tttatggcct tcaacattta cggtcagtag
    15121 tttcatcaag cactcctctc atactaattc agcatgatag ctcaagcttt gtctgaaatc
    15181 atccaaatgt tcgagcacct tactcatgcc ttcaataacc actaaatcgg gattatctac
    15241 aactgatacg ggcattttaa attcagttga aagtgcgcga tccaaaccat gtagctgggc
    15301 tagaccacca gaaagtagga gccctttatc caaactatct accaataacg ctggcgctgc
    15361 ctcttcaagc acatgaaaca acaaggtggt gtattcaccg gtgagctgtt gagctagttg
    15421 aaccaggtgc ttcgcagcaa cttcttgttt gatcggacga agagtttgac ttgattttcc
    15481 cattaccacc tgcattctgt tttgatctgg aagtaaggaa aaaacttctc tttttatttt
    15541 ctctactacc atggaatcga caattaagtt gtggtcgtta ttcagtaaca agctcatttg
    15601 ctcggcaaga tagtgacccg ccagcagtga ctggcgggtc accataatac ttcccaaaga
    15661 aaccacccca gcttccacaa caccatctcc caaatgaaga taaaaacttc cagttgcgtc
    15721 agcggtatgc acgcccgatc caatagccgc agccaaaggc tgtgcaatcg taatcacttc
    15781 tactgctcca agtttttcaa ataccgaacg aataaccgag cgatttgcct gcgtactctc
    15841 cgcagttgtg ctgactaaca ccgtaggttg caataacaaa gtcggcttaa aatacgggcg
    15901 caaccacact tttaagagtg caattaaggc tgattcatcc attattacac ccgcttcgac
    15961 tggccaaatt atctgtacat cttggttttg tcgtccggca atttcgactg cttctttacc
    16021 agtcgctagc acttctcctg atggcgcaat tgctaagcaa gttggttcgt tgatcacaac
    16081 acccttgccc tctacccaaa ttcgagtatt taacgatcct aagtcgaatg caaactgcct
    16141 gaaaagagtg cggaaaaatt tcatctttac cagtataata acagcatgca cgttccacgc
    16201 aacaaaggat tgttatttac tgttttttcc gcactagtta ttgttgttgg tacgtacatg
    16261 gccatacggt atgcaaaagg cggattccgc gtcacagaca gtggtataag ccaagagaca
    16321 ggcttactct cagtcagctc actccccact ggtgcagaag tgtacatcaa tgaaaaatta
    16381 gtcactgcca ccagcgacac gttataccta gacccagatc aatatgaagt tgctattcgc
    16441 aaagatggtt atacagaatg gaaaaaaata atcactttag aaaaagaggt agttagtcaa
    16501 gccaacgctc tactcttccc cagtgctcca agtctcactc ctctcacttt tactggcatt
    16561 gaaaatcttt ctccttctcc ggatggtcaa aagattcttt tttatagtgc ttcaaattcg
    16621 gctcaaaaga aaaatggctt gtatgtgtta gagctaagtg acaactcttt atcacttcaa
    16681 cgtggtgctc ggcaaatcag tcaagacagc tcaaccatag atcttgccac cgctaaattt
    16741 atatggtcac cagacagcac ccaaattata gtttttggtt caaaccgaga ggtattacta
    16801 gatgtgagtc gtttgaatga tctagatgct ctttcggata ttagttttcg cagaaaacag
    16861 ttgctaagcg attgggaaga tgaaatggcg ctgagagaac aacagttctt gggtaaattc
    16921 cccccagcaa taatcaaaat agctacgtcc agcgccacca atgttttctt ctctccagac
    16981 aaaaagcgct tgttgtacac cgctacggaa tcagcaacgc ttgatccaaa cctaattcct
    17041 gcactacctg gagccaactc tcaaccagaa gaacgccaac ttcaagccgg aggaatttac
    17101 atttacgatc gagaggaaga taaaaatttc cgtgttggta cccagcaaga taaaacagta
    17161 tttcctgcca aaagtttact ttcaacgttg tttgaacaaa atcaagctac acccaacacc
    17221 attgccacca ccagcgctcg tcgcagtctt gaggctagct cctcttctga attggcggat
    17281 ctatatcgaa attatcattc ttctctcttt gccgatacgt accaatggtt tcccgattcc
    17341 aaacatattt tttctgccga agacagtcgc attgtgatta aagaatacga taatacaaac
    17401 gccgttaccg tttattctgg accatttttc caaaacttta tctatccatg gcccgacggc
    17461 agtcgtttat tgatagccac tcactttaat ccagagacac cgaataattt gtatgcgatt
    17521 gaattacgat aatggcactt attccttagg ttctcaatta gatgtagggt agaatactag
    17581 atacagattc ggggtgtagc tcagcccgcc tgcaacgctg tgcgtagcac tgcgggcagg
    17641 atggctagag cgcgtcaaaa cttaagacaa cttaacatac aatacggggt gtagctcaga
    17701 tggctagagt gcgtcgttcg ggacgacgag gtcgctggtt cgagtccagt caccccgact
    17761 atttattcat atctcaaagc gttcagtgca gaaatcttat gcgctcgcct cgccgggtag
    17821 agacccgtca acatccctac caaggccgct aataaaaaca caaacagcaa aaacaaaatt
    17881 ggaaattgaa ataacgacaa cgtcttatca gatctgctca gcatagaaat ccaattcagc
    17941 gagtttccga gcaccacgcc aaacaacact ccgagcactc ccccagccgc accaatcaaa
    18001 gatgattcta caatcaacaa tcgtcggata tcgcgatcgg ttgtacccaa ggtcttcatc
    18061 actccaattt cgcgagttcg ctcaagcaat gacacggtca gagtattaaa cataccaaac
    18121 aatgccacaa taaaggcgat catgccaaag ctacccagta aaaatcgcat aatggaaaac
    18181 agcttattaa cttgcaacaa agtatcggca atgctccggg tccgaaaacc tagggcgcca
    18241 atcctctctc gtaccccttc aagcacttca gaattatccg ccaatacctt agctaacgag
    18301 tatttactta ctcccatact ttctaaatca gaaagcggcg caaacaccat agattgctct
    18361 ggcaaatcga acacacccac aatcgtataa tccgtggctt gcgatgtgac tttacctttc
    18421 atacctgcaa ccaaacctcc actcaaaatg tactgaacgg acaagttctt accaattaac
    18481 gaatcaggag ttagttttaa tgcatttaaa aaaccggtcg acaccaggat ttctttacca
    18541 cctcgttttg caacactcgc cactgccaaa gtagccgatg cagaaccact ctggggttga
    18601 gactgaataa tcattgactc caataattgc tgttctgagg ctgaactcgt ggcggtttta
    18661 tcgagaacca cacccacaac attatcttcg gtattatctc ctaaaacttg ctctcgatct
    18721 tctcgctctt gagcttccaa gtgaaggact tccaaggctt tttcagcgag ggcctcctgc
    18781 atcgtcagga ggcccactga gttttgcgtc aagtaaccca ctcccagctt ctgcttccct
    18841 tcagcttcca ctataggagc aaacactgtc ggcgcctgtt ccatatacaa cggtaaaccc
    18901 tgctcaatct tgagccactc acccaaccaa gtaccagtag aaagctgatg agcacgacca
    18961 gcagtgccaa ccgtgtcata cgctccgccc caaactttat acccttggtg ccgttctaat
    19021 attgaacctc tcacgtaacc cacaatttca ctttttgtat ttggctctgc tctcagtggt
    19081 acatacacat catcgctgat tctaaaatta accggttggt cactcacgcg gtcacccgct
    19141 atgggctcaa cctcaatgct gacaccagaa accaatccag cactagctgt tgccagtcta
    19201 gacagatcga gcacttctcc agaatacgcc atttctgctt cttcagaaaa acgctcgcca
    19261 gccactaact caatatgcga agcatccaag aaatcatttc tagcacctac cactactacc
    19321 tcagtctgcg cgtcaccata cttcactgag ccggctaaat tgaccgacat tgacacattt
    19381 tccaccccag caatttgact gagttgttca atagcaggct tatctatttg ataggcagtt
    19441 gaagtggatt ggacatcagc aagtcgtaat gaatttggct gcaccagtcg tttcgtcaca
    19501 atcgattcca gtccataggc aaaaccaact aagaccacaa tggcagccga tccagcagca
    19561 atagcgccca ccgtcacggc ggttctagaa aaagaacgca taatattttt ccgaccgatg
    19621 agaatcaaat acgaccaaga tagttcacct ggttgcccaa accagtgacg tctaacctga
    19681 aaaagaatgg cttcaccaat aagcttaata gactttagtg gatggtgtac aaatctttta
    19741 aagaacgcga gacctttctg acctacttcc aaactaacca gtgtaagctg ctgcaacttt
    19801 tttcgactat tttctaatag ttttaagcga taccgaagag gcttcgttgg aaccctaatc
    19861 ggagtaaaaa ctctttcaac cgtagctgag acagttttat ggctagtgtt ttgatcagtc
    19921 atgttggtca taaataattc gtccgtcttt caaaacagtg cgccgagtag cgatgggcag
    19981 caaactcaaa ttatgagtca ccatcaaaat taacttactt tgttcccggt ttaatttggc
    20041 taacagagca atcatgtcct cgctcgcctc cgagtctaag ttaccagtgg gttcatcggc
    20101 tacaatgatt ccggggtcag tgaccatcgc tcttgccaac gccactctct gctgttggcc
    20161 accagatagt tcgcttgggt gatgatcagc ccattctgct aactcaacct gctccaacca
    20221 attcagagcg gtttgtctgg cagatgtagc ggattcgccc gctaaccaaa gtgggtacga
    20281 cacattttct attacactta aagatttaat ccagttgctc tgttgaaaca ccaccccgac
    20341 tttttctctt ctgaatcggg tacggccttg atcatcaaga tccttataca aatcaacatc
    20401 tctcataatc acactaccgg cagatggtgg ttcgagtccc aagatgctat gcaaaagtgt
    20461 tgatttacct gacccagatg gtccagaaat aatgacaaat tctcctgagt agaggttgag
    20521 tgatacgtcg tgcaagaccg ggacaacaga ctcaccaacg ggaaatgact tgccaacttt
    20581 atccaccttt atgactacgt ggtgtgaacg agcagctttt ggaggcagtt tcttctccaa
    20641 cgctaccaca tcgaccgcca cgagtggtaa ctctggcttt gctcttttct gatgagtgat
    20701 tatttttttc ttgggcatta ctggagtcct aaaaagccaa agataccttc taagtttttg
    20761 agaatcaact caaaggcagc tgcttgcgct gtgggtgtga ctactacggt atcctctgtt
    20821 tcactcgtgt tacctgtttg atctgtggtt ttgattttta agtgataggt agaagtggga
    20881 tccaagtcac gtaacacaat catgtgatcg gtagttgcct cagacgacgt ttgcgttgct
    20941 tgtgggtact cagaaccagt gcctgctccc cacaggactt gggagaaagc aggttcatcg
    21001 gtagtaaatg agaggacaat ctcggcttta ctctctactc cttctcccac gatgggagtt
    21061 tcaatcacta ggttttctac agaaggaggc ttggtgtctg ctcctgtgac gtaacggatg
    21121 ggatcagaga ctgcttgatt gccatactcg tctctgccac gaatggtaaa gacatagaga
    21181 gaactgtcag acagattggt gatttgaacg acatgacgat tactaatggt ggagcgatca
    21241 agatactgag gagagccaag cggtacgact ggtaatctag ctaattctgt ttgactgagt
    21301 ccctgaagaa ctgacgagtt actatagtta atttctgatt ggctggcaaa gctcggatca
    21361 attttaactg cctgatattc cacactggca gtagtgggca cattagtact ccaggctaca
    21421 ttaatcgcag tggcgttttg agcttgatcg gttttaaaga caactgcggt gactttaggg
    21481 aaagtgagtg tggtgaaggt gtagtcatct gacgtaatat cagtgccatc gatgtcgacc
    21541 gctctgattc tgaagtgata ggtcaccgag tctttcagtc ctgaaagctt cgttgagtag
    21601 gtgcccgatg atcccactcc aactgaaaca gaagcaccat aggcagtact ctctccatac
    21661 tcaatggtgg cagaagatag agagacagcg ctccagttga tgatagctga ggcgagtgtc
    21721 aggtcagtca cttctacatt agagatagtg ggtagcgctg aagttgtaaa ggtgtaatca
    21781 ctactgtaag ctatctctcg gtcatagccc atgaggattg aatcatccag cgcttgaact
    21841 cggtagtgat agacagtgcc tggagttaga ccggtgagtt taatagagtg gttacccact
    21901 gcgactgtct ctgctactga tgattcataa ctggtggttt tgccatactc aactgctccg
    21961 taggcaattc tgccagtact ccaagtaata gtggcgacgg tagaaccaac tgtcacattg
    22021 ggagtgccac cagcaagtgg tggtgtggtg tacttacctt ctggagcaag cgttacgatg
    22081 ttggatgaga gtgaggtgga gttagcgttg tcgattgcaa aaactttgta ggaatactct
    22141 tgggactgcg ttaagtcagc atcggtatac gcagtagaag tagtttcgcc aatctttgta
    22201 aaggttgtcc catcagttga acggtgaatg gcataaaagt taggtgtccc accagttact
    22261 gcttgtttcc aagcgagcgt taatcgccat acttcagcgt cgcggtcgga agcatcagag
    22321 atggtcagat tggttggtgc tcctggagca atggtggaag cataaaactc tgcagtagca
    22381 taggcttcaa agttaacatt tcccctaccc gtgttggtat taatgttgtt gtcttctgcc
    22441 actaggtaga aggtgttctt accttgttga gttgctaaag caatgttggt caaagttctg
    22501 tctgccgtct gtacactggt ggtttgcgtc atggtctcaa ctgaaggagt acagttcaca
    22561 cagtagtagt acttcgctag ctcaccactg taggaagcgg gtttgtccca ggtaacagta
    22621 aagatgttct ctaacgcttg agcatcacta ctggtaacag gcgaaatggc cacgtttact
    22681 ggcgcagtgg gagctgactc gttgtagtag aagggtgaga ctcctaggtt tgatatctca
    22741 gcggaaacgt taccagcaaa gtctacggtt ctgacaaaga agaagttttg accttcttgg
    22801 taagcagtga tgttactggc tagagttacc aagggatctc ctggctcatt tgattttgtc
    22861 caagtactaa aggctccttc ggtagctcct gtcttgtact catagtacgc aagacccgat
    22921 tgattaagac cttgatcaga agcagctggc cagtagaagc taaactcatt ctgagaggtg
    22981 tacccaacgg tagtagatga gacggaaaca ggtgaacgag gtgaagtact gtcatagcgg
    23041 taggtgaaga gagtgacagg ctcagataca ttacctgagt tgtctttggt ttgcatacgt
    23101 aggtagtaat cagcatcact caagctgcaa ccagcgggtg tagttcccac actaaagact
    23161 cggtcatcag attcagcaaa gcggtaacag gggttggcta agttggcaga gggagtagct
    23221 gcttggttgg tggttaagag aatatggtag ccatcaatac cactactgga ttgcccagca
    23281 gctggatcat ctactcccgg ttcccaagta aacttgggag ttgagtagtt gtaccaattg
    23341 tttgaagtta acgtggccga tgaggaggca tggttaacgt agcctaaagc tacctgagcg
    23401 gggttagcag gcggagttga gtcttctaca taggtcgtag acatagcgga gataacggga
    23461 gactgactct gatcgccatt actacttaaa tctgctttcc actggaagta gcgtcttgga
    23521 tccgaagcaa tcccacctgt ccaactgccc tcatctacac tacctgttac tgcttgccat
    23581 tcactccaca gagagttatc catactggtg cgggtaaagt aattaatccc tcccccattc
    23641 aaaatattag aagcactcca cggcacactc ccccacgctc cattcgcccc caggtcaatc
    23701 acaggcgagg tatacgtacc actcgcggaa tatctatcca cagcctcact accactactg
    23761 tgagtcacca aaccttgttg gtacaaagca gaaacttcag agggagtcag ggcttggttg
    23821 aagatacgga ggtcggaaag ggttgagtct ccctctctag tccatccgcc accaatatgc
    23881 agttgcgtag atgaagacat tgttccatcc cacgtcgcgg tgttaacgaa aaccccattg
    23941 aagtatagtt tgagattatt tgcaccatcc caagtaatag tcactaagcc ccacctatct
    24001 ttaactaccg atccaaaaga tggttcactt gcccatccgc catcactttc acgaaccgca
    24061 acgctgtaag cagcactttg cttcacaaca aatatccaat catcaactgt aaatatactt
    24121 ttgctatttg tctcagatga atttggtttc acccacatac ttaacgagcc tcgactggcg
    24181 ctaaaacttc cagagtttac atatctcaaa ttatccactg tcctgctact cgcactctca
    24241 ttcgcagtcc cactccagct atacccactc cccaaactac catcggcata cgtagtcgca
    24301 tacgtctttg cctccaactg cactccatcc acatagaccg tcttgcctgc caaggcttgg
    24361 acaccataaa gcagtgaggc atctgtggta gctgcagagt aagtcagtct ccaccagcct
    24421 cctcccagat cagtatatgc agcaggagtt actacatttc caccaaagac taactttgca
    24481 cttgttgacg ctaccgttcc acccacattg cctgtcgtcc catcgtagac ataagcannn
    24541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnact taaacttaat caccccccta
    24661 tctgcactca aacccgtaat cggctgcgta agcgtatccg ccccatccac tcgcaaccca
    24721 cttgcttcag tactgttcgc ctgattggca ggagtgactg tgagagagac gtcggagagg
    24781 gcaaatgcat aagcatcatc caaaagaaat gaggtggtac tcccgccctg gttgaaccaa
    24841 taaagttcgg ggtttgaggt tattactctc tgcacagtct gagatgctgt ccaacttgga
    24901 taacttattg agtattcttg ataagctgag tttgaatgct gttgaatatg agtcccttct
    24961 aacagacgaa tgttactacc attcaacatc ttgctccaat gaccccagct aaaaaacttt
    25021 ccagttgtta aagcaggtga aatatcatta agtccataaa acctattgtg ctctattgag
    25081 ccattcaaac atgcagagcc tgaatgacag tccatgcttt caactgaacc gccactagat
    25141 ccatttgtta cccatcctgt aggaacatat ggcgccgaac caatacctgt ttccaatgaa
    25201 ggattggtta taagattggg aaatacctcc acctgatgcc agtaacaggt gccagaacta
    25261 gcagtattga taagctggac ttgatttgct tctgcagcag gtgattccca agtaaagata
    25321 tagacatcgg gatctgtacg agtactggtg gttgtaccgt taaggtgaga tatttctgtt
    25381 gaaccatcag ctctagtgat ttttacttga ggattacaag ttccatcaga gtgaccaaga
    25441 gcacgcagta catagtcccc tccgtttgta gcagtaaatg agcggtagat accttgatta
    25501 gtagcattag aagtaaactt atagccacca gagaagattt tttcggaaga tgagagggct
    25561 gttacttcaa aatttgtaag atcgtatttt gatgttaaat atgatcttac agcagattct
    25621 tctactgagt tcagctctct gtcatatact aaaatttctg ctacatctcc aacaaaatat
    25681 cttccatata agcttgagtc tacccctatc tttaaattag aaattactct cccagaatta
    25741 tgtgatgccc gcaaaattga tggggtatag accaagggat tcccagcacc atcactagca
    25801 ccagtaataa cccatcctgg agctatagag tcatctccat gagttgctga gccaactatt
    25861 ctttgccatt gagttcctcc aatagagtct ctttttgaca ctaccagtga tgtgactcct
    25921 ccctcagttg cacgaatagt acccacagaa aggtaatcgt tagtaccatc aaatcgaatc
    25981 actggttttc catcgatctg attcgtttta tatattggtt tattggccga aatagtttgc
    26041 gtggcgtgaa agccacttcc acttaaatct gtcattgatg acacttgatc accatcgctc
    26101 aagcccataa ttgaacctgc atctaaccag agcttgagtc cagaaatttg ttgtggagac
    26161 gcaagccaac catctactcc ggcatctcca gccatcataa acccaccagt attggtggta
    26221 gatgtgtaaa ggaggttgga agagtgagga tgactccaag cattaccagt acctgttgtt
    26281 agtgcaccag tactagcatt ggtgggcaga gcaaagacga catcccaact accagttact
    26341 aacgtgggat ctttaccagt tcctgagtta taaatctgcg ctatttcatc agcattacct
    26401 acatctgtcc catacgtgcc atcaaacagt gggcgtcgat agatggttag gccttcaatg
    26461 atggagtcca taggatctaa acgatagaag ccaccaccaa caacatagga agtagttcta
    26521 gtaaactttt ccacatcatt gacggtgata atgaagttgt aattagaatt gccaattttt
    26581 tctttctgat cccaacgaat tacaatatta tatgtcgaac ctgcagtcca atcagatata
    26641 tcaaatagat aagaccatcc ataatcgtaa agtaccagtc gattatcaga atgtttgaag
    26701 agtgcaaaat ctgtagcttg tccaaaaata taatgcgttt ttccatcatt gccattccat
    26761 tcaggagtaa tccaaaaaac tattgagcct tgtgacctat caatcgtgct agtcaacgat
    26821 ccagcgatat tgctttgaga ccaagagata gaatccccat tgttccacgc gtagctagca
    26881 tctgacttac cctgcaccat cggacccgca gcaaacggcc cggcggaatt tctcttcgcc
    26941 atggaaggag taaaggtggc agaagcagtg gcgtagttga agtagttggt accccagtta
    27001 agagtggctg atgaggcgtt ctcaaaagaa ggattggtaa ttaaattggt ggtaccttct
    27061 tctataaaga cgccttgatg ggatgcatag gtgctagcag gaatagcacc agtgctggaa
    27121 ctagagttat caacccctac taacccactc acatacttag cacc
//
LOCUS       DAOQZP010000005        27127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_2076_length_27127_cov_5.129543, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000005 DAOQZP010000000
VERSION     DAOQZP010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 27127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27127
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2076_length_27127_cov_5.129543"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PKH60_00525"
     CDS             <1..765
                     /locus_tag="PKH60_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNQ16567.1"
                     /translation="DTICDRCAGPRQFGEYQIKTWNEEYHPNISMSDALAKSDNIAMI
                     FVAEKLGPQKLRNYFRDFGIGEPTDIELQDDTGTPFPNSLRPVELATVSFGQGISTNS
                     MQLVRAVSVIANQGILMKPKILSAMYDPITGNNISVEPKELRRVVSAQTAQQVTQMMI
                     YSAQHGEAQWTYRPNHTVAGKTGTSQVAVKGGYDEEQTIASFVGFAPANNPAFVMLVK
                     LTAPTSSIWAAETAAPLWYDIAEELYLLLQIPPDRQ"
     gene            782..2023
                     /locus_tag="PKH60_00530"
     CDS             782..2023
                     /locus_tag="PKH60_00530"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNQ16568.1"
                     /translation="MLSLVLYRLKRPYHFFKTGLLRGLPAQLKYGFPEKKLKCIVISG
                     TDGKTTTATLIHHLLTSNSIKAGLLSTVSARIGSKEIDTGLHVTAPEPQDLFFVLQQM
                     VEAGCTHVVMEMTSHGAYQFRNWGIKPWLGGITNITHEHFDYHLNYQEYARAKLQQFR
                     GASKVFFPEGDKSYSIARKMLRSVQVKFYSATTKLPQGVSRAIKSRFAEPYNQQNAIL
                     ASLIATEVGLSPKNIATAISSFTGVPGRLEEIPTTKGFRVFVDFAHTPNAVESVLTHL
                     KSQSKGKLIAILGCAGLRDRTKRPSMGKIAASLADLTIFTAEDPRTENLWSIIGQMKS
                     QLETLHNKVLSIPNRGEAIHFAITRLAKRGDTIAILGKGHEKSLCIGSKEFAWNDRNA
                     VQHIFQTSEVPTLRAALTDAT"
     gene            2026..3030
                     /locus_tag="PKH60_00535"
     CDS             2026..3030
                     /locus_tag="PKH60_00535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HNQ16569.1"
                     /translation="MRTKPTTYQDQLVTTIGGGTGTYPVVAALRTLCNHLTCIVAVSD
                     SGGSSARLRDEFGFPPVGDLRQSLTALANPTSQHLLQKLLLYRFNKGRELKGHNLGNL
                     ILTALQDMTGTTTQALETAAQLFQFHGRVIPITEDSVNLKVFYRDGTSIIGEHLLDEV
                     DAPLKPIRKIALIPKPKTNPQALEILSQSDHLIIGPGDLFASLEAVLVAPKVSKIIQN
                     SNAKITYIVNIMTRRSQTAGMSATDHVVEIEKVIKRSITNIVINNRSPKSEQLAPYQL
                     VDEYPVVDDLGNDRRVIRAPLLTYTPTKQSASDVVPRSVLRHNSNQLQKVLRRILKNQ
                     "
     gene            3030..4511
                     /locus_tag="PKH60_00540"
     CDS             3030..4511
                     /locus_tag="PKH60_00540"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNQ16570.1"
                     /translation="MQTNLTQSLQSFKHCYSIGIKGVGMTALAQCLLDKGIAVAGCDV
                     SEEFVTEKILSSLPIRVDSLEVQKLPIDTDFVIYGSAHQGKEHFLAQQAIKRSIPLYS
                     HAQILGMLTQEKDTVAVCGVGGKSTTSALLTWILENSQEDPSYHVGVGEITGLPRTGR
                     WHTTGSLFITEADEYAENPQAVAQGEEIIPRFHYLQPKIIICTNLLYDHPDVYASFET
                     TANTFYSFFTQLKQNGILLWNADDVQLQTVVQKLHDNRPDILTVSFGYSKSAEVLIHD
                     LGYETGAHLVEFISNKQSLIKQNSQLIRFSLPGLHNLQNAAAAWTCLTLIQLPDQVIA
                     QHMATFQSTSRRFQLMNTIDESFFYDDYAHHPTEIAATLSACKALFPNHKLIVLFQPH
                     TFSRTEALLPDFANAFSKADTLLLIPIFGSARENIGTITLDDLAKAIKTARSVRTDIR
                     IATSITNAVEQLGSIDTKKAVVITLGAGDVYTIHDLLAQKGHA"
     gene            4508..5587
                     /locus_tag="PKH60_00545"
     CDS             4508..5587
                     /locus_tag="PKH60_00545"
                     /inference="COORDINATES: protein motif:HMM:NF014872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNQ16571.1"
                     /translation="MKTFDGDTFLRGLLAELPQLNYQTETPLAPYTTVKIGGPAELLL
                     ETKSSAELATVVKYVRSAKVTLTILGWGANTLISDAGIRGVTIINKSSSLTVLPDPVS
                     DSTAISQITHRWHQIDGANYDFFDYYSGEESRVLVECESGTPLPFAINQSLQLQLTGL
                     EWFSRIPATIGGAIYNNVHGGTHFISDQIYSVLILDEQGKEQTLKPSDMEFDYDYSRF
                     HHTKEIILAAKILLFKGDVEKAKAAAIAWAQKKHHQPQKSLGCVFQNISQSDQQRLQL
                     PTTSIGYLIDKVLRLSGYTIGGAQISKSHAAFIENIGNASAMDYLQVIEHIQSAAKKT
                     CNITLKPEIFFRGFSQNDLSRIQVT"
     gene            5603..6940
                     /gene="murA"
                     /locus_tag="PKH60_00550"
     CDS             5603..6940
                     /gene="murA"
                     /locus_tag="PKH60_00550"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNQ16572.1"
                     /translation="MSHFKITGGVPLKGSVRIGGAKNASFKLMIASLLAQSESRLLNF
                     SQISDVHLVGKIIQYLGGTVRKAGERALFIDPSTLSNYFIDPAHGEQGRFSTMFLPAL
                     LHRFGEARVPAPGGDKIGKRPLDRHFEGLTALGVQINYQDGLFIAKTQGMHGAKYRFN
                     KNTHTGTETLIMAATLAKGKTILENAALEPEIDDLISFINKMGGRITRKPGRVIEIEG
                     VKTLHGAIHKLLPDRNEAVSYACAALATKGDLIIENAVPKHLRAFLKALDKAGGSYEV
                     GDYGIRFFYTKPLQAVQLTTAIEPGFMTDWQPLWATLMTQATGTSTIHETIMASRFQY
                     MEELIKMGARIESFQPKVNNPELRYNFNLQDDLPNAQHAIRIFGPVTLKAGEFRVKDL
                     RHGATIVLAAMTAKGVSTIHNIAQIERGYEDLEQRLQSMGAQISLVSDLSSDN"
     gene            6965..8008
                     /locus_tag="PKH60_00555"
     CDS             6965..8008
                     /locus_tag="PKH60_00555"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNQ16573.1"
                     /translation="MGPFATALGILMLAFVVTSILIIPFVNLLYRLKFLRAHQITTDA
                     FGKLTPIFDKFHRSKAGVPVGGGLLVIFVVSVLFAITIPILSYSGRDITSVYRNLNAE
                     MYIIFFTMISFGLLGLYDDIKKFFRFKPVGFFGLTLKFKLILQIILASIISILLYKTL
                     GINFIHVPFIGIIPLGALFVPFATITIVSFSNAVNITDGLDGLASGSLMISLFGLWFL
                     SAAILDVPLTVFLALWLGSLISFLYFNVFPARIFMGDVGSLAFGATLAVVGLLLGKVV
                     ALVIIGFIFVLEISSSLLQLVSKKWRKKKIFPAAPIHLTLQAYGWEEPKIVQRAWLLQ
                     IMLTIFGVWLAVL"
     gene            8018..9121
                     /locus_tag="PKH60_00560"
     CDS             8018..9121
                     /locus_tag="PKH60_00560"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HNQ16574.1"
                     /translation="MKKRKLLSTGRLLNRWQSLFLSILCILLALGTLFMLDISLVESA
                     ATFDDPYYLFKQHLVGLAIAAIFFTIGILVKPKVWLRLGPYAYLFGLVLLLLVFIPGI
                     GLDLNGAKRWFALGSFRLQPVEFFKGALIMFLSGWLYKHKQIVPFLFALITPATLLLL
                     QPDMGSLLIVLGIAVILFFLAGGKLSTLATLGGALIPILLILIITSPYRMQRLTSYIN
                     PDSDPLGASFHIRQITLALGRGGMWGKGLGNSNQKYAYIPESTTDSIFAIVAEEVGFT
                     GSVTICLLFLTLFYSGYRIIQTSDAPNSERLLALGMLSWIMLQTTLNLAAVAGLVPLT
                     GIPLPFFSYGRSSQVMTLFACAIIIRTGRSFKS"
     gene            9118..10230
                     /locus_tag="PKH60_00565"
     CDS             9118..10230
                     /locus_tag="PKH60_00565"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ16575.1"
                     /translation="MKLLITGGHLTPALAFIDYLQLNHPEVSIVFVGRLYARKRSKQP
                     AHEKEEVDKRSIPFIAFDSGKLVTAHPFFQLFHLILFVVTIPKAIRLLLQTKPTHVLT
                     FGGYLGVPLAIAAWMLRIPIVTHEQTRGFGLANRFISFFAKKIALAHSSSMKFVDLTK
                     SIVVGNPIRLGLKQKETDKPIWLSTKVDRPILYITGGSQGSEIINTTTIQILDIITKQ
                     WLVIHQCGSASNERNYLEELSRETTHLAPSAQKRYFVREWISDKELAWIYHHADLVIS
                     RAGANTVEELIQFGLPSILIPLPFAHQDEQMQNAVAMKKIGGAIIIEQKQLSPELLLQ
                     TISQLKNQLGKIKVSIRNNQKTTNSCELLFKLINNA"
     gene            10223..10984
                     /locus_tag="PKH60_00570"
     CDS             10223..10984
                     /locus_tag="PKH60_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16576.1"
                     /translation="MPNIPQLKFQMVTTFSIILGVLLLFGLATNLLTPRYISCRIDSQ
                     AECPKEVLADLLFIMDSQLLFSDIQKQIVELNSIKGRYEIVKVSKDLWGDIIIDLKPL
                     STAYSLITNNAAYAVTSDGALLQSTDSPELITDQTCTLTFSQDTAVEKFFPEQTSLIS
                     ENQINHDLHSQMLTILNSLQSRQLQCLKIQPVDQQTWVIWLPTVDQIVVNPTEIDSNL
                     NRLSFLLDTTLLTEQRPANSFLDLRFALPVLRNSL"
     gene            11053..12357
                     /gene="ftsA"
                     /locus_tag="PKH60_00575"
     CDS             11053..12357
                     /gene="ftsA"
                     /locus_tag="PKH60_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNQ16577.1"
                     /translation="MARADIVTAIDVGTDKCVTIIAAIDKELHSIRVMGVSAIPSRGV
                     KKSQIIDLEQVLISVTESLDSAERMAGVEIKNAYVSISGAHVSSLNSKGVVAVAAPDQ
                     EISNDDVTRVIEAARAISLPSDREVIHIIPKSFSVDSQEGIKDPVGMTGVRLETEAHI
                     ITGLTTTMRNLEKCILDLGINLDGFVFSGLASAEVVISETEKELGVVALDIGAGSTSM
                     CVYVDGSLEYSASLPIGARHITQDIALGCRVSLDAAEKIKTHLSTDNLKPINPHPGES
                     KQDFSKRKKQHDSLQLSDIGVIDESEYISKKSVVEGIMVPRMKELINLVGEQLEKKRL
                     LALVPAGLVLTGGGAQTVMFTEVAKHTLQLPARIGLPQELQGLTSDIHSPTYSTAIGL
                     LVYGTKQMQTTDKASFNFSKMPFGKLTEFPTKIIQFFRSLVP"
     gene            12429..13697
                     /gene="ftsZ"
                     /locus_tag="PKH60_00580"
     CDS             12429..13697
                     /gene="ftsZ"
                     /locus_tag="PKH60_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNQ16578.1"
                     /translation="MALVKPTNTTFAKIKVIGVGGGGGNAVNSMIDSGQIQGVEFISI
                     NTDAQALLTSKADTKLQIGSNFTKGLGSGADPDVGKTAAEESYDKIKELLFDTDMVFI
                     TAGMGGGTGTGASPIIAEIAKESGALTVAVVTKPFTFEGVRRMQAAEEGIEGLRDSVD
                     TLIVIPNQRLMDIVDKKMTLLDAFRLADNVLGQGVQGISDLITVPGLINVDFADVKTV
                     MAESGSALMGIGEATGENRATAAARMAIASPLLEISIEGAKGILYNIIGGSDLTMTEV
                     SDASNIIANAADPDANIIFGATINEEMGSKIKISVIATGFDEKHMKYSAGARPYFGAY
                     SKESNQRLSQNQTPDSQPMIDRAPSGFVQPITVSQLNQQNQSSANDDQNDQPAIPSKD
                     VFKPTTPDLSDDFSDDFEIPAFLRNNKNKS"
     gene            complement(13816..15666)
                     /locus_tag="PKH60_00585"
     CDS             complement(13816..15666)
                     /locus_tag="PKH60_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16579.1"
                     /translation="MLNRTKQPITIYSSQAKKPVAGLRLLVVAVFSLIGGIFASTFGS
                     ILLEKMGIANPAPQKVQAVYGSSARCSNQDGEWRITAVGESYNPDAASYTGFFDLVPP
                     SSVTVGTDVAIDGLKFFCPLATGQDGGGNPSTNGQCGLQWYVHTITGERIQFTQDTST
                     FHFNAGDTSPRRIEYTLTNAPPRCGGYQLDLGFKQIVAGGQTTQCSGIVLGNLAHATC
                     TSPGCTVSGFTGESCNIPAYCPSRALGAEFMNDGVHVSLQRDVFANSPLKPMIKYRKL
                     NDNGTAGDWQDGCAYGAYTCIIPPSFYANFSPQEKLQIIVNIHLVRTDGAELNVCSWN
                     VGWVPAQAVNNEYFLGGCTNLCQQLLTIPVVPPSPTPTSTPTATPTPTATVTPRPSAT
                     ATPTPTATVTPRPSATATPTPTATVTPRPSNTPTPTPTATSTPTPTATPTNTPTPTPV
                     PECNSLCRTNQECPSNLFCYFGTNPITRTGHCRLPSNPEDLQCRPTPTATPTPTSTPT
                     ATPTPTATVTPTPTATVTPSPTASVTPSPTVTITPSPTVTVTPNPSATATPTPTITPT
                     PEIRTVVIYRDGRPVEPHQPIDTGVNAGEAVASVSIFLSGLAWFIKQRVF"
     gene            15842..18694
                     /gene="ileS"
                     /locus_tag="PKH60_00590"
     CDS             15842..18694
                     /gene="ileS"
                     /locus_tag="PKH60_00590"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNQ16580.1"
                     /translation="MSKTNPQPTSVQLNQLPSTPNFASLEELVQSYWTENDTFRASVQ
                     LRNKKKQYVFYDGPPFATGMPHYGHLLGSTSKDVIARYWTMKGFRVERVWGWDCHGLP
                     IENMIEQKLGITGGKREIESRGIDTFNEACRSEVLRLDQEWEKIISRLGRWVDFEHSY
                     KTMDCSYMESVWWGFSELNKKGLIYQGKRVILYCSRCATPLSNFEIAMDNSYETITDT
                     ATTYKYPIVGQASTYILAWSTTPWNKIVTPALAVHPELEYVQVLQNNEHYILARSTLA
                     ILNDEPYTIEKSFTGKELTALKFIPHYDFYPKRKASEKFGVIVADPFVTAEEGTGVVT
                     LAVYGEDDFRVMKAEHIQMAEHIDEEGKIKVDVTPWVGLSIAEVNPLVNQELLSRNLL
                     YSDRPHTHSVPVCYRCSTRLYYAPIPAWFIDIQKLKPKLLSENQKMNWYPDHLKNGRF
                     AKGLENAPDWNISRSRYWGTPMPVWQAPTGEQRVIGSIAELQAWAVDQNAVKNISDLH
                     RHHVDQIEVWVDDAKTIRGTRIPEVFDCWVESGSMPYASRHYPFENKELFDETYPAQF
                     VSEYIAQTRAWFYTMHVMSVGIFDKMAVENTLTTGTILAADGSKMSKSKKNFPDPMEV
                     IHKYGVDSLRLYLMSSSVMKGENSNFNEKEVSDIRKRVFLIWWNIFSFYRLYAQPTDV
                     PPKPIAPPHIMDRWILSRANSLILQVTSYLDSYDVVRASRVLIEFVSELSTWYIRQSR
                     DRFRNPETRAESCQLLASVLIALSKTAAPLTPFFSELIYQNLINTSTSLHLTDWPATK
                     QSLIDNELEKDMQLVQKICEKAHAIRKEKAIKVRQPLATLQVRSSTNQLSDELIEILK
                     QELNVKSVDWKMSTEEVEVELDLTLNADLIAEGQAREIIRSIQGLRKEAGLSIDAETI
                     VTLPTWPKSWQSEIELKTKTKLKQGPSLQITTEPLE"
     gene            18691..19767
                     /locus_tag="PKH60_00595"
     CDS             18691..19767
                     /locus_tag="PKH60_00595"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNQ16581.1"
                     /translation="MNRWFIPSILLVISSISVVFINSLVPELTSKHLLSIFLGFVVFV
                     TTARLPIQQVFRRPWHLYLFTCILLLIPLFLPVQIRGTSRWIPIIGSFRLQPSQIAFA
                     LIGISISSLTVKFSKDTAQNIKSTAKQLGLIIIPTLLIAVAPNLGTAVFFFFCMSMII
                     FFSGIPWKHIAFLLAGVICFSYIAWLFLLQPYQKQRITTFLQPSQASQNETYNVRQSL
                     IAIGAGGLSGSGFGKGSQSQLRFLPEKHTDFVFAAFSEEFGFIGSALLVLIYLVLSLY
                     LLKESYSIQSLEQKYFVLFTSTIVFFQAMINISTNIGLLPVTGITLPFFSYGGTSFIA
                     FGLIFGTAQNCIQKEKRYSLLHIV"
     gene            19797..20114
                     /gene="rplU"
                     /locus_tag="PKH60_00600"
     CDS             19797..20114
                     /gene="rplU"
                     /locus_tag="PKH60_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNQ16582.1"
                     /translation="MSYAVVQLQGKQFIIEPGTILEVDRLDAEEKKAVEITDVLLLVT
                     DKEVLVGTPTVTQAKIKTEFVKDLRGEKIRVAKYKAKSRYRKVRGHRQDLTQIKVVSI
                     EAK"
     gene            20126..21331
                     /locus_tag="PKH60_00605"
     CDS             20126..21331
                     /locus_tag="PKH60_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16583.1"
                     /translation="MSSPAVQHFFLLGSTPELSWLELSRTFPDICGLIRISNQLAGSR
                     LLPPQISATKVLTLLGGVVKIAEVISMLPDDEEQIISEIVTYLKQGESRIHFALAEWG
                     RDHLPKVEPQLIKERLTQEGKSVRYRTGSRTGLSAAILLHESDLQELIVISTNDGAYL
                     AKTSAVQNIDQWQIEDRSKPFAQHKRGLLPPKVGRMMVAIGLGKTIFDTDNQKSPLAL
                     WDPFCGSGTILFEGLLLGLPIVYGSDIDTESIEGTEKNWQWFKNAFQNQLADKQQLVS
                     FQSDATSVQTPPFDSSSISMIVTEPFLGKPTPDFRQIANIQSGLERMYKGMFKEWKQW
                     LKPGASVVCVFPKWQISGDYKQKTFDLHRLIDFLKPIGYTTASDPVVYARPGAVVQRE
                     IWHFTYQNQ"
     gene            21338..21589
                     /locus_tag="PKH60_00610"
     CDS             21338..21589
                     /locus_tag="PKH60_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNQ16584.1"
                     /translation="MSHVKASGKVAQHSQRKRKGLRLGLKKSGGQVAVAGNILIRQRG
                     AVYQPAKGVGMGKDHTLYSLVDGVVTFGKRRGKTTVSVL"
     gene            21650..22477
                     /locus_tag="PKH60_00615"
     CDS             21650..22477
                     /locus_tag="PKH60_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNQ16585.1"
                     /translation="MSKDVYQLLDITLLQPNPFQPRGKIHEEEVSELAESIKSYGILE
                     PIVVAHTPAGHQIIAGERRWRAAQLAGLTEVPVIIRETTPKGMLEMALIENVQRINLN
                     AIERAKAFQQLLRDFRYSAVELANKVGKSEAFVSNSLRLLNLPDAVKDGVVGKQITEG
                     HARALAGLEDKKLIIEVYKRLLKENASVRRAEELVRQVKQASETKTTTAPGKRIKVDP
                     ALLSRWQKSFEEFLSHSSPRVNLSRSTRQTRLTVTLRGNLEETQKDLEQILQLVQKH"
     gene            complement(22474..23049)
                     /gene="lepB"
                     /locus_tag="PKH60_00620"
     CDS             complement(22474..23049)
                     /gene="lepB"
                     /locus_tag="PKH60_00620"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNQ16586.1"
                     /translation="MSRFFASLGSLVIDLTETLVIGFSLFLVVYLFFMQPHQVNGQSM
                     VPNFQSGEYVLTDKISYRFRAPKRGEVVVFHAPPEAHCQEGTGCDFIKRIIGLPGDQV
                     EVKNNGIYVNNAKLQETYIPSGYDTYPGQYTRDKVIILEQNEYFVSGDNRPYSSDSRA
                     WGPITIDEIVGRAFFVYWPPKAAGMIENVNY"
     gene            23123..24334
                     /locus_tag="PKH60_00625"
     CDS             23123..24334
                     /locus_tag="PKH60_00625"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNQ16587.1"
                     /translation="MGYSGKVFSGFSWQTTLRVVLIGVGFARIFILARLLTPQDFGTF
                     AIVLISLGLLEAITETGINLTIVQSKHQIEYFIDTAWIISIIRGAIISTLMIASSFFI
                     ALFYQKPELVLMIILTAAVPLIKGFINPTIGALHKNFQFSTQVFFQFSRYAAEAIIAI
                     VLVLIWPSVWSLVIALIVAALFEIGISMVFFHPKPKFRFQMARARIILNNSKTLTLQA
                     LLSYLVENVDDLLIGKTLGPHMLGLYHNGYALTHKLLYDSAKSASYGLLPTYTKLIDD
                     PERLKRGLLRSTLFFSSLLICGTIVIALLARPIVLLALGEKWQSIIPIISVLAFAALL
                     QSISTLLSTFLYATKKYHGITFGLLVQVIILICSLWYLSMQFGLIGAAWAIVVARMAG
                     LPFLIWAVIKK"
     gene            24344..25108
                     /locus_tag="PKH60_00630"
     CDS             24344..25108
                     /locus_tag="PKH60_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16588.1"
                     /translation="MKKIPLSVLILANQLSPKLMAAIHSVTFADEIIIIDTSGTKAIS
                     FPKQISAIVKQVKPVTNFAATRNHSLSFAHNNWVFFLDSDEVCTCPKPDLLIPMLQKD
                     CSGFQVSREDHFLGKVLHHGETAKSWPTRIINKTACAYQSIVHEKVICEGTIQSINPE
                     ILYIEHFPHDSIYSFFKKISWYSKLEAEQRNTPTIRLIFELFTYPTGKWIANLLFDQG
                     LLDGYRGLVYATLMSYHSLLVRLFQLEKQIIKRHRD"
     gene            25500..26282
                     /locus_tag="PKH60_00635"
     CDS             25500..26282
                     /locus_tag="PKH60_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16589.1"
                     /translation="MYLIFPDLRALKSLGWDDHYYRAVGTLLDPNFTGIIIVVCLLNW
                     VGLFSIRTIRSIIFQYSLIGLLTISLGLTFSRASWLTYLFCLGLILVPVLIRLKFQFL
                     QATIVKLILFSIALLIITITVAPKPGGAGVDLSRSATVVARQQHDQSLLDTNRVRLII
                     GGGLSSAPNSELRYHARLPNNLGMIILAFSGIPGLVTFFWGLSQTFRMIIKQYPLMLI
                     PAIAVLFFAQFNAALTEPFVLLICGIQLIVLTAEKKLFKPFA"
     gene            complement(26285..27049)
                     /locus_tag="PKH60_00640"
     CDS             complement(26285..27049)
                     /locus_tag="PKH60_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16590.1"
                     /translation="MKQTEYRVVNRNDSPMSPTPTTSRIVKRERDKLLKQTMTWSLIA
                     LGLLLLFLFIILPNAPRLLASIVDGGAVFGQEDTIPPQVPIFNSPPMATSTKQIEVSG
                     FAEAKSQVLIVLNGNQVADLTTSDDAVFKQLIDLQEGENYLQAFSKDAAGNESNLSKE
                     YVISLDTEAPSLALSETIQDGMQVVGKENKSFTISGLTEQNAKVAINDRFLFARPDGS
                     FSYQIQLNEGENLLKLVITDKANNIFEQELRVNFKL"
     gene            complement(27053..27125)
                     /locus_tag="PKH60_00645"
     tRNA            complement(27053..27125)
                     /locus_tag="PKH60_00645"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27090..27092),aa:Thr,seq:cgt)
BASE COUNT         8072 a         5738 c         5434 g         7883 t
ORIGIN      
        1 gacacgatct gcgatcgttg tgcaggaccc agacaatttg gagaatacca aatcaaaacc
       61 tggaatgaag aataccaccc caatatttcg atgagcgacg cactagccaa atcggataat
      121 attgccatga tctttgtggc cgaaaaactt ggtccccaaa aactacgcaa ttatttcaga
      181 gatttcggca ttggtgagcc tactgatatt gaactccagg acgatactgg cacgccattt
      241 ccaaatagct tgcgtccagt tgaactcgca actgtttcat ttgggcaggg aatcagtaca
      301 aatagtatgc agcttgttcg tgctgtaagc gtaatcgcca atcaaggtat tttaatgaaa
      361 ccaaaaatac tttcagcaat gtatgaccct atcaccggaa acaacatttc tgttgaaccc
      421 aaagagctta ggcgagtggt gtctgctcag actgctcaac aagttacgca aatgatgatt
      481 tactcagcgc aacatggcga agcgcagtgg acgtaccgcc ccaaccacac agtggctggc
      541 aaaacgggaa cctcacaagt tgctgttaaa ggaggctatg acgaagaaca aactattgcg
      601 agttttgttg gttttgcacc tgcgaataac ccggcatttg tgatgcttgt caaactcacc
      661 gcaccaacga gttccatctg ggccgccgaa actgctgctc cactttggta cgatatagcc
      721 gaagaactct atttactcct gcaaatcccc ccagacaggc aataactgct ataatgttac
      781 catgttatcg ttagtgttat atcgtttaaa acgaccatac cactttttta aaacaggttt
      841 gcttcgaggc ctacctgctc aattgaaata tggatttcct gaaaaaaaat taaagtgcat
      901 tgttatctct ggtacggatg gaaaaactac caccgcgaca ctcatacacc acttgctaac
      961 aagcaattca attaaagctg gtttgctttc taccgtttcg gctcgtattg gttccaaaga
     1021 aattgatacc ggcctgcatg tcactgcacc agaaccacaa gatttatttt tcgttttgca
     1081 gcaaatggta gaggcaggtt gcactcatgt tgtgatggaa atgacctcac atggtgcgta
     1141 tcaatttcgc aattggggta taaaaccttg gctgggtgga attacaaata ttactcatga
     1201 acattttgat tatcatctca actatcaaga gtatgcccgc gctaagttac aacagtttcg
     1261 tggcgcttca aaggtgttct ttccagaggg cgataagtca tattcgatcg cgcgaaaaat
     1321 gctaagatcg gtccaagtaa aattctactc tgcaacaact aaattaccgc aaggtgtttc
     1381 gcgcgcaata aaaagtcgtt tcgccgaacc ctacaaccaa caaaacgcga ttttagcatc
     1441 tttaattgct accgaggtcg gactttcacc caagaatatt gctaccgcaa tttccagttt
     1501 taccggagtg cctggccggc tagaggaaat acccacaacc aaaggattcc gggtgtttgt
     1561 agactttgcc catacaccaa atgccgttga gtctgtatta actcatctaa aatcgcaatc
     1621 gaaaggaaaa ctcatcgcca tacttggctg cgctggttta cgagatcgta caaaacgacc
     1681 gagtatgggg aaaattgcag catcactagc tgatctgaca attttcaccg ccgaagatcc
     1741 tagaacagaa aatctctggt caatcatcgg tcaaatgaaa tcacaattgg aaacgttgca
     1801 caacaaggta ctctctatcc cgaatagggg agaagcgatt cattttgcta taacacgctt
     1861 ggccaaacgt ggcgatacaa ttgcaattct tggcaagggc cacgaaaaaa gcctctgcat
     1921 cggctccaaa gaatttgctt ggaatgaccg gaacgcagtg caacacattt ttcaaacaag
     1981 cgaggtacct actttgcgtg cagcgctcac tgatgccacc tagttatgcg tactaaacca
     2041 acaacttatc aagatcaatt agttaccacc attggaggtg gcaccggaac gtatcccgtt
     2101 gtagccgcac tgcgcacact ctgcaatcac ctaacctgca tagttgcagt ctcagattct
     2161 ggaggttcat ctgcacgcct gagggatgaa tttggttttc ccccagttgg ggatttgcgc
     2221 caatcactta ccgcattagc aaatcccacg agccaacatt tacttcaaaa acttttgctc
     2281 taccgcttca ataagggtcg tgaattaaaa ggccacaacc taggaaacct aatacttacc
     2341 gccctccagg acatgactgg tacaaccact caggcattag aaaccgccgc tcaattattt
     2401 caatttcacg gacgagtgat tcctattacc gaagattctg tgaatctaaa agtgttttat
     2461 cgagacggta ccagcattat tggcgagcat ctacttgatg aagttgatgc acctctcaaa
     2521 ccaatccgca aaattgcact tataccgaaa ccaaaaacca accctcaggc actagaaata
     2581 ctcagtcaat ctgatcacct tattatcgga cctggagatc ttttcgctag tctggaagca
     2641 gtactggttg caccaaaagt aagtaaaatc attcaaaact cgaacgctaa aattacctac
     2701 attgttaata tcatgacgag aagaagccaa actgctggaa tgagcgccac cgatcatgtg
     2761 gttgagatag aaaaagtaat taaacgatcg attaccaata tcgtcataaa caatcgaagt
     2821 ccaaaatcag aacaacttgc tccctatcaa ttggttgatg aatacccagt tgtggatgat
     2881 cttggcaatg accgaagagt aattcgagct ccactcctta cgtatacacc aactaaacaa
     2941 agtgcgtcgg acgttgtacc tcgatccgta ctccgtcata atagcaatca actacaaaaa
     3001 gtattgagac gaatattgaa aaatcaataa tgcaaacaaa tctcactcag tcgcttcaat
     3061 cttttaaaca ttgctacagt attggtatta agggagttgg aatgactgcc cttgctcagt
     3121 gcttactaga taaaggcata gctgttgctg gctgcgatgt aagcgaggag tttgtaactg
     3181 aaaaaatact ctcttccctt cccattcgag ttgacagttt ggaagttcaa aagttaccaa
     3241 ttgataccga ctttgtaatc tatggaagcg ctcaccaagg taaagaacac ttcttagctc
     3301 aacaagcaat caagcgctca attcccctat attcacacgc tcaaatactt ggtatgttaa
     3361 ctcaagaaaa agacactgtt gcagtttgtg gcgtcggagg taaatcaacc acatctgctt
     3421 tgcttacctg gattctagaa aattctcaag aggatccaag ctaccacgtt ggtgtaggag
     3481 aaatcactgg gttaccaaga acgggccgtt ggcatacaac tggcagccta tttataacgg
     3541 aagcagatga atatgctgaa aatccccaag ctgttgcaca aggagaagaa atcataccgc
     3601 gttttcacta cttgcaacca aaaataataa tttgcaccaa cctgctatac gatcacccag
     3661 acgtgtatgc ttcctttgag accacagcaa atacctttta ctcgtttttt actcaactca
     3721 agcaaaatgg catattacta tggaatgcag atgacgttca actgcaaacc gtggtacaaa
     3781 aacttcacga caatagacca gatattctca ctgtaagttt tggttattcc aagtctgccg
     3841 aagtactaat acatgatctg ggttacgaaa caggagcaca cttggtcgaa ttcatttcca
     3901 ataaacagtc actcattaaa caaaattctc agctaatccg attttctcta ccaggtttgc
     3961 acaatcttca aaacgctgcc gctgcctgga catgtttgac actcatacaa ctcccagatc
     4021 aagtaattgc tcaacatatg gctacttttc agtcgacatc acgccgtttt caactgatga
     4081 atacaataga cgaatcattt ttttatgatg actatgccca tcaccctacc gaaatcgcag
     4141 caactttatc tgcctgcaaa gcactatttc ctaatcataa attaatagtt ctatttcagc
     4201 ctcatacttt ttctagaact gaagctctgt tacccgattt tgccaatgcg ttttcaaaag
     4261 cagacaccct gttgctcata cctatctttg gatctgctag agaaaacatc gggacaatca
     4321 cgcttgatga cttggctaaa gccatcaaaa cagcacgatc agtaagaact gatattagga
     4381 tcgccacctc tattacgaat gcggtggaac aattgggatc aattgatacg aaaaaagctg
     4441 tcgtgattac actaggtgcc ggtgacgttt atactattca tgatctactt gcccagaaag
     4501 gtcatgcatg aaaacatttg atggggatac atttctacgg ggattactgg cagaattacc
     4561 acaactaaat tatcagactg aaactccact agctccctac actacagtaa aaattggcgg
     4621 cccagccgag ctattacttg aaactaaatc aagtgcagaa ttagcaacag ttgtgaagta
     4681 tgtccgttca gccaaggtta cactcactat tctgggctgg ggcgccaaca ctttaatttc
     4741 tgacgcaggt attcggggtg taacgattat aaataaatcg agctcgttga cagtattacc
     4801 agatccagtc tcggacagta ctgcaatctc tcaaattact caccgatggc atcaaataga
     4861 tggagcaaat tatgattttt ttgattatta ctctggagaa gagagtagag ttttagttga
     4921 gtgtgaatcg ggtacaccac tgcctttcgc aattaatcag tcgttacaat tacaacttac
     4981 tggacttgag tggtttagcc ggattcccgc cacaattggg ggtgccattt acaataatgt
     5041 ccacggaggc acccatttca tatcagatca aatttattcg gtcctcatac ttgatgagca
     5101 gggcaaagaa caaactctaa aaccatccga catggagttt gactatgatt attctcgatt
     5161 ccaccataca aaagaaataa ttcttgctgc aaaaatactc ctttttaaag gagatgtaga
     5221 aaaagccaaa gcggcagcta ttgcctgggc gcaaaaaaaa catcaccaac ctcaaaaatc
     5281 attgggttgt gtttttcaaa atatatctca aagcgatcaa caaagattgc aactgccaac
     5341 cacgagcatt ggttatctaa ttgataaggt tttacgtttg tcaggatata cgattggcgg
     5401 cgctcaaatt tccaaaagtc acgccgcctt cattgaaaat attggaaatg catcggcgat
     5461 ggactatctg caagttattg aacatattca atcagcagcc aaaaaaacat gtaatattac
     5521 tctaaaacca gaaattttct tccgaggttt ttcacaaaat gatctttcaa gaatccaagt
     5581 aacgtaaaga gaggatagta atatgtcaca ttttaaaatt actggcgggg tcccgctcaa
     5641 aggctcagta agaatcgggg gggcaaaaaa tgcctcattc aaacttatga tcgcctcact
     5701 ccttgcccaa tccgaatctc gattgcttaa tttctcgcaa attagtgatg ttcatttggt
     5761 gggtaaaatt attcaatact tgggcggtac agtgcgtaaa gcaggcgaac gggctttatt
     5821 tattgatccc agcacactgt caaattattt tatcgatccg gcccatggag aacaaggcag
     5881 attttcaaca atgttcctac ctgccttgct gcatagattt ggcgaagctc gggttccagc
     5941 gcccggagga gacaagattg gcaagcgtcc attagacaga cattttgaag gtctaaccgc
     6001 attgggtgtt cagattaatt atcaggatgg tcttttcatt gcaaagactc aaggcatgca
     6061 cggagccaag tatcggttta ataaaaatac ccacactggc acagaaacac ttattatggc
     6121 tgccacgctt gcgaagggca aaacaattct tgaaaacgca gcgctcgaac cagaaatcga
     6181 tgatttaatc tcttttatca ataagatggg cggaagaatt actcgaaaac ctggccgagt
     6241 gatcgaaata gagggcgtaa aaactcttca tggggcaatt cataaactgt tacccgatcg
     6301 aaacgaggca gtttcatacg cttgcgccgc attagcaaca aagggagatc tcataattga
     6361 gaatgcggta cccaaacacc taagggcatt tctcaaggct cttgacaaag ctggaggatc
     6421 ctatgaggtc ggagactatg gtattcgatt cttttacacc aagccactcc aagctgtaca
     6481 actgactacc gcaattgaac caggcttcat gacagattgg caaccactgt gggctacgct
     6541 tatgactcag gcaactggta ccagcacgat tcatgaaact atcatggcga gtcgatttca
     6601 atatatggaa gaactaatca aaatgggagc gcgtatcgag tcattccaac caaaggttaa
     6661 caacccagaa ttacgctaca actttaattt acaagacgat ttgccaaacg cccaacatgc
     6721 gatccgaatc ttcggaccag tcactctcaa agcaggcgag ttccgcgtta aggatcttcg
     6781 ccacggggct accatcgtgc tggcagctat gacggcgaag ggggtgagta ctattcataa
     6841 tattgcgcaa attgaaagag ggtatgaaga tcttgaacaa cgcttgcaat cgatgggagc
     6901 tcaaatttca ctcgtttcag acttgagctc tgacaattag tcagtacaat agaactacaa
     6961 aatcatggga ccattcgcaa ccgcactcgg aatcttaatg ctcgccttcg tggtgactag
     7021 tattttaatt attccatttg ttaatttact ctatcgatta aaatttttac gcgcgcatca
     7081 gattaccact gatgcatttg gcaaactcac tcctatcttt gacaaatttc atcgatcgaa
     7141 agcaggagtc ccagttgggg gaggattact ggttattttt gtggtttcgg ttttatttgc
     7201 tattactatc ccgatacttt cttattccgg tcgagatatt acgagtgtat atcgcaatct
     7261 gaatgccgaa atgtacatta ttttctttac gatgatctcg tttggcctac ttggattata
     7321 cgatgatata aaaaagtttt ttcgctttaa accagttggt ttttttggcc taacattaaa
     7381 atttaaattg atcctgcaaa tcattcttgc atcaataatt tcaatcttgc tctataaaac
     7441 acttggaatc aattttatcc acgttccatt cattggaata attccacttg gagccctttt
     7501 tgttcctttc gctacaatta ctatcgtgtc gttttcaaat gctgtaaata ttaccgatgg
     7561 acttgatggg ctcgcctctg gcagtttaat gatttcgttg tttggtctat ggttcttatc
     7621 cgcagcaatt cttgacgtgc cattaacagt ttttctcgca ttatggctgg gatcacttat
     7681 ttctttttta tattttaatg tttttcctgc tcgaattttt atgggggatg ttggatcact
     7741 ggcgtttggc gcaacactcg cagtcgttgg cttattgctt ggaaaagtgg ttgctctagt
     7801 tatcattgga tttatctttg tgctcgaaat ctcaagttcg cttcttcaac tagtctcaaa
     7861 aaagtggcga aaaaagaaaa tttttcctgc agcaccaatt catctcactc tgcaagccta
     7921 tggttgggaa gaaccaaaaa tcgttcaaag agcctggctt ttacaaataa tgctaacgat
     7981 ttttggtgtt tggctcgcag tgctgtagct atttcgcatg aaaaaaagaa aattgctctc
     8041 aactggtcgg ttgctcaatc gttggcagtc actatttctc agtatattat gcattctgct
     8101 ggctttgggc actttgttca tgcttgacat atctctcgta gaaagcgctg caacttttga
     8161 cgatccctat tatcttttta aacagcatct cgttggtttg gccattgcag caatcttttt
     8221 taccattggc atactcgtaa aacccaaggt atggcttcga cttggacctt atgcatatct
     8281 tttcggttta gttttacttt tattagtatt tattccaggc attggattag atttaaatgg
     8341 ggcgaaaaga tggtttgccc ttgggtcatt tcggttacaa ccagttgagt tttttaaagg
     8401 agcattaatc atgtttctct ctggatggct atataaacac aaacaaattg ttcccttctt
     8461 gtttgctctg atcactcccg ccactctcct tcttcttcag ccagatatgg gttcattatt
     8521 gattgtttta ggtatagcag tcatcctttt tttcttggca ggtgggaaac tatccacgct
     8581 cgcaactttg ggaggtgcac tgattccaat tttactcatt ctcataatca catctccata
     8641 ccgaatgcag cgtttaacca gttatatcaa tcctgattct gatccactgg gagcctcgtt
     8701 tcacatacga caaataactc ttgcgctcgg tcgtggcggt atgtggggaa agggattggg
     8761 aaattcaaat caaaaatatg cctatattcc cgaatcgaca actgactcaa tttttgcaat
     8821 agttgccgaa gaagtagggt ttacaggatc ggtgacaatt tgcttgttat ttctgacatt
     8881 attctattct gggtaccgaa ttatccaaac cagtgatgct cccaattccg aacgtctgtt
     8941 ggcactcggc atgctcagtt ggatcatgct acaaactaca ctcaatcttg ctgccgtagc
     9001 aggcttagtg ccactcacag gaataccgct gccatttttt tcttacggac gttcttcgca
     9061 ggtgatgact ttgtttgcat gtgccataat cattcgtaca ggtagaagtt ttaaatcatg
     9121 aaattactga ttactggtgg tcacctcact ccggcacttg catttattga ctatcttcaa
     9181 ctcaatcatc cagaggtgtc tattgtgttt gttggtagat tgtatgcaag aaaacgctca
     9241 aaacaaccag ctcatgagaa agaggaggta gataagcgca gtattccttt tattgcattt
     9301 gattcaggga aacttgtaac agcccatccc ttttttcagt tatttcatct catactcttt
     9361 gtagttacta ttccaaaagc aattagattg cttctacaaa ctaaacccac gcacgtgctt
     9421 acttttggag gatatcttgg tgtacccctg gcaattgctg cttggatgct acgaatacca
     9481 attgtgactc acgaacaaac aagaggcttt ggcctcgcca ataggtttat tagttttttt
     9541 gcaaagaaaa tcgcactcgc tcattcaagc tcaatgaagt ttgtagatct cacaaaatca
     9601 atcgtggttg gaaatccaat tcgtctgggg ttaaaacaga aagagaccga taaaccaatt
     9661 tggttatcaa caaaagtaga ccgaccaatt ctctatatca ctggcggctc tcagggttct
     9721 gaaattatca acactacgac tattcagatc ctggatatca taacgaaaca gtggctggtc
     9781 atacatcaat gtggatcggc atcaaatgaa agaaattatt tggaagaact ttctagagaa
     9841 accacacatt tagctccaag cgctcaaaaa agatacttcg ttcgagaatg gatttcggac
     9901 aaagagttgg cttggattta tcatcatgca gaccttgtta tctctcgtgc aggcgcaaat
     9961 acagtcgaag aacttattca atttgggctt ccctctatct tgattcccct cccatttgcc
    10021 catcaagatg aacagatgca aaatgctgtg gcgatgaaaa aaattggtgg cgcaataata
    10081 atcgagcaaa agcaactcag tcccgagctt ttacttcaaa caattagtca attaaaaaat
    10141 caacttggta aaataaaagt ttcgatacgt aacaatcaaa aaactactaa ttcatgcgag
    10201 ttgttattca aactcattaa taatgcctaa cattcctcaa cttaaatttc agatggtaac
    10261 tactttttcg attattcttg gagttttgtt actttttgga cttgcaacaa acctactaac
    10321 tccacgatat atttcctgcc gcatagattc ccaagctgag tgtccaaaag aagtgctagc
    10381 agatttgcta tttattatgg atagtcaatt attgttcagc gacatacaaa aacaaattgt
    10441 agagttgaat tcaattaaag gaaggtatga aatagtgaaa gtttcaaaag atctttgggg
    10501 agacatcata attgatctaa aacccctatc cacagcatac tcactgataa ctaataacgc
    10561 agcatatgct gtaacaagtg atggcgcact actccaaagc acagattcgc cagaacttat
    10621 tacagaccaa acatgcacat tgacttttag tcaagatact gcggtggaaa aattttttcc
    10681 tgaacaaaca tcattaattt ctgagaatca gataaatcac gatcttcatt cacaaatgct
    10741 aactatactt aactctcttc aatcaagaca actacaatgt cttaaaattc aacccgttga
    10801 tcaacaaaca tgggtcatct ggttaccaac agtagatcaa atcgtagtca atccaaccga
    10861 aattgattca aacctaaacc ggttgtcgtt tctactagac acaactctgc taactgaaca
    10921 gcgcccagcg aattcattcc tcgatcttcg cttcgcattg ccggtactac gcaattcact
    10981 ctagatttgc tatgcttata gatagtattc gtgtagtatt ttattgtaaa tgaaatggta
    11041 taacatataa ctatggcaag agccgatatt gttactgcaa ttgatgttgg aactgataaa
    11101 tgtgtgacca tcatagctgc catcgataag gaactccact cgatccgagt gatgggtgtg
    11161 tcggctattc cctctagagg tgttaaaaaa agtcaaatca ttgacttgga gcaagtactc
    11221 atttctgtca ccgaaagtct tgactcagca gaacgtatgg cgggagtgga aatcaaaaat
    11281 gcatacgtct caatttctgg cgcacacgtg agttctctca actcaaaggg tgtggtagca
    11341 gtggcagcgc cagaccaaga aatttcaaat gatgatgtga ctcgggttat agaagcggct
    11401 cgagcgatat cattaccttc tgatcgcgaa gtaattcata tcattccaaa aagtttctca
    11461 gttgattcac aagaaggaat taaagatccg gttggcatga ctggcgtgag acttgaaact
    11521 gaggctcata ttattaccgg tttaactaca actatgagga atttagaaaa gtgtattttg
    11581 gatttgggaa taaatctcga tggtttcgtt ttttctggtc ttgccagcgc cgaagtcgtg
    11641 atttcagaaa cagaaaaaga gttaggagtg gttgcgctcg atattggagc aggatcaacc
    11701 tcgatgtgcg tgtatgttga tggctcactt gaatactcgg cgagtttgcc cattggagca
    11761 cgccatatta ctcaggatat tgcgcttggc tgtcgagtca gtttagacgc tgcagaaaaa
    11821 attaaaacac atctttctac cgataattta aaaccaatta atccacaccc tggtgaatca
    11881 aaacaggatt ttagtaaacg taaaaaacaa catgactcac tacagctgag tgatattggt
    11941 gtgattgacg agagcgaata tatatcaaaa aaatcggtag ttgaaggaat catggtacct
    12001 cgaatgaaag aattaataaa tttggtgggt gaacagttgg aaaaaaagag gcttttagca
    12061 ttggttccag ctggcttagt tctcactgga ggcggcgctc aaaccgtgat gttcaccgag
    12121 gttgccaaac atacacttca gcttcctgcc agaataggtt tgccacaaga attacaagga
    12181 ttgacgagcg acattcatag ccccacttac tcaactgcga ttggcctatt ggtttatgga
    12241 acaaaacaaa tgcaaactac tgataaagcc agtttcaatt tctcaaaaat gccattcgga
    12301 aaattaaccg aatttcctac caagataatt cagttctttc gttcattagt tccctgatct
    12361 gtgactagta gttgatctgc gggacagacg tggtacactt gcaaaagtaa ttccatctat
    12421 accaaactat ggcattagtg aaaccaacca ataccacctt cgcgaaaatt aaagttatcg
    12481 gagtaggggg cggtggtggc aatgcggtaa attccatgat tgattcagga caaattcaag
    12541 gcgtggagtt tattagtatt aacactgacg ctcaagcact cctcacctca aaagccgata
    12601 ccaagcttca aattggtagt aattttacaa aaggattggg ctcaggcgct gacccagatg
    12661 ttggcaaaac tgctgcagaa gaatcctacg acaaaattaa agaattactc tttgatacag
    12721 acatggtttt tattacagcg ggtatgggag gtggcactgg aactggcgcc tcaccaatca
    12781 tcgcagaaat tgcaaaagag agcggagctt taacggtggc tgtggtcact aaacccttca
    12841 cgttcgaggg tgttcgaaga atgcaagctg cagaagaagg aatcgagggt ttacgagata
    12901 gtgtagatac cttaattgtc attcccaatc aaagactgat ggatattgta gacaaaaaaa
    12961 tgactctact cgatgccttt cgacttgcag acaatgtttt aggccaagga gtacaaggca
    13021 tctcagatct gattaccgta ccaggtttaa ttaatgttga ttttgctgat gtaaaaaccg
    13081 tcatggcaga atcaggctca gcgcttatgg gtattggtga ggctactgga gaaaatcgag
    13141 ctactgcagc tgccagaatg gctattgctt caccgctcct cgaaatttcc attgagggag
    13201 ccaaaggaat tttgtacaac atcattggag gcagtgattt aacgatgact gaggtttcgg
    13261 atgcttcaaa tattattgct aatgcagcag atccagacgc aaatattatt tttggagcga
    13321 cgataaatga agaaatgggt tcaaaaatca aaataagcgt cattgctaca gggtttgatg
    13381 aaaaacacat gaaatattcg gctggcgctc gaccatattt tggcgcctat agcaaagaat
    13441 caaatcaacg tttatctcaa aatcaaaccc cagattcaca accaatgata gatagggcgc
    13501 caagtgggtt tgttcaacca attacagtct ctcaactgaa tcaacaaaac caatcgagcg
    13561 ccaacgatga tcaaaacgat cagccagcaa ttccatcaaa agatgtgttt aaaccaacca
    13621 ctccggactt aagcgatgac ttctccgacg attttgaaat acccgcattc ttgagaaata
    13681 ataaaaataa gtcttaaata ccgcagtaat ttatattatt tgtagtcttc aaattgagtc
    13741 ccataattgg aagcaaaaag cgccgattgg tttttaccat atcggcgcta ttaaattcat
    13801 tatctaggag ttaaattaaa acactctctg cttaataaac caagctaatc ctgagagaaa
    13861 tatgcttacg cttgcaaccg cttctccagc attaacaccg gtatcgattg gttgatgagg
    13921 ctctacaggt ctgccatcac gataaatcac tacagttctt atctcaggcg taggagtgat
    13981 cgtaggtgta ggagtagctg tagcactcgg gttaggcgtt accgtcaccg ttggtgatgg
    14041 cgttattgtc acagttggag acggagtaac tgacgctgtt gggctagggg ttacagtagc
    14101 cgtaggagta ggtgtgacag ttgccgttgg agtaggtgta gctgtgggcg tacttgttgg
    14161 agtaggcgta gccgtgggag ttggacgaca ttgtagatcc tctggattac ttggtaaccg
    14221 acagtggcca gttcttgtta ttggattagt accaaagtag caaaataagt tgcttggaca
    14281 ctcctggttg gttctgcaaa gtgagttgca ttctggcact ggagtaggtg ttggtgtatt
    14341 tgttggagtt gctgttggcg taggagtact tgtagccgta ggcgttggtg taggtgtgtt
    14401 gctaggtcta ggtgtgactg tagccgttgg cgttggagta gctgtggcac ttggtctagg
    14461 tgtgactgta gctgtaggtg taggagtagc tgtagcactt ggtctaggtg tgactgtagc
    14521 tgtaggtgta ggagtggctg taggagtgct tgttggagta ggagagggtg gaacaactgg
    14581 aattgtcaat aactgctgac ataaattagt acatccacct aaaaagtact cattatttac
    14641 tgcctgggct ggtacccagc ctacattcca gctacataca tttaattccg caccatctgt
    14701 tcgtaccaga tgaatattga caattatctg taatttttct tgtggagaaa agttggcata
    14761 aaaactagga ggaataatac aagtatatgc accatatgca caaccatctt gccaatcacc
    14821 cgcagttcca ttgtcattga gttttctata tttaatcatt ggttttaacg gcgaattcgc
    14881 aaacacatcc ctctgcagtg aaacatgaac cccatcgttc ataaattcag ctccaagtgc
    14941 tctggatgga caatatgctg gaatgttgca tgattcacca gtaaaaccag aaactgtgca
    15001 tcctggagaa gtacatgtgg cgtgagctaa attacccaaa actatgccac tacactgtgt
    15061 ggtttgtcca ccagctacta tctgtttaaa tcctaagtca agttgataac ctccgcatcg
    15121 aggtggagca tttgttagag tatattcaat tcttcgtggc gaagtatcgc ctgcattaaa
    15181 atgaaatgtt gaagtatctt gagtgaactg aattctttca ccagtaatgg tatgaacgta
    15241 ccactgaagg ccacactgac cattagtact tgggttccca ccgccatctt gcccagtggc
    15301 caaaggacag aagaatttta atccatcaat agcaacgtcg gtacctactg tgacagaact
    15361 tggaggcacc aaatcaaaaa atcccgtata ggaagcagcg tcgggattgt aactctcacc
    15421 cacagctgta attctccact ctccgtcttg atttgaacaa cgagcactac taccatagac
    15481 tgcttgaact ttttgaggcg cagggttggc aattcccatt ttttctaaca agatagatcc
    15541 aaacgtcgaa gcaaatattc caccaattaa tgagaaaacc gccacaacca gcaatcttaa
    15601 acctgccacc ggctttttgg cttgggagga gtaaattgtg attggttgtt ttgttctatt
    15661 tagcattagg tttactctca tgtagtatat attaattctt ttatattcgt ttagatatct
    15721 tttatatctt ttgcgcattg tattacatag ttataataat gtcaatacta tttattatag
    15781 gcaactttag atgtatctcg gttgactctt cgcctgctta ttgcgatact atgggaattc
    15841 aatgtcaaaa accaatccac aacctaccag tgtccaatta aaccaactac catccacacc
    15901 caattttgca tctctagagg aactcgtgca atcctattgg actgaaaacg acacatttcg
    15961 cgcttcagtg cagcttagaa ataagaaaaa acaatatgtt ttttatgatg gaccaccatt
    16021 tgcaaccgga atgccacact atggccactt acttggatca accagtaagg atgtgatcgc
    16081 tcgatactgg acaatgaagg gatttagggt tgagagagtc tggggatggg attgtcatgg
    16141 tttgccaatt gaaaacatga tcgaacaaaa attgggtatt actggtggta aacgtgaaat
    16201 tgaatcaagg ggtattgata cctttaatga agcgtgccga tctgaggttt tacgcctaga
    16261 ccaggagtgg gaaaagatca tttctcggtt aggacgctgg gttgattttg aacatagcta
    16321 caaaaccatg gattgttcct atatggaatc tgtctggtgg ggattctcag aactcaataa
    16381 aaaaggatta atttatcaag gtaagcgtgt cattctgtat tgttctcgct gcgccacacc
    16441 cttatccaac tttgaaatcg ccatggataa tagttatgaa accattaccg ataccgctac
    16501 aacgtacaaa tatccaatcg tgggtcaggc gagtacatat attctggcct ggtcaactac
    16561 tccttggaat aagattgtaa ctccagcgct agcggtgcat ccggagttgg aatacgttca
    16621 ggtgttgcaa aataatgaac actatatttt ggctcggtca actcttgcca tcctaaatga
    16681 cgaaccgtac acaatagaaa aaagtttcac cggtaaagaa ttaacggcat tgaagtttat
    16741 tcctcattat gatttttacc ccaagcgtaa agcctccgaa aagtttggcg tgatagttgc
    16801 tgatccgttt gtaactgccg aggaaggtac gggcgtggtt acactagcag tttatggcga
    16861 agatgatttt cgggtcatga aagccgaaca catccaaatg gcagaacata ttgacgaaga
    16921 aggaaaaata aaagttgatg taacaccctg ggtaggattg tcaattgcag aggtcaatcc
    16981 cctagttaat caagaattgc tctccaggaa tctgctctat tctgatagac ctcataccca
    17041 cagcgttcct gtttgctacc gttgtagtac tcgactctat tatgctccaa taccagcgtg
    17101 gtttattgat atccaaaaac tcaaaccaaa attattgagt gaaaatcaaa aaatgaattg
    17161 gtatccagat cacttaaaaa atggtcggtt tgcgaagggg cttgaaaatg cacccgactg
    17221 gaatatttca cgttctagat attggggaac acctatgcca gtttggcaag ctcccactgg
    17281 agaacaacga gtaataggct cgattgcaga acttcaagcc tgggcggtcg atcagaatgc
    17341 ggtgaaaaat atcagtgatt tacaccgaca ccatgtggac caaattgaag tttgggtgga
    17401 cgacgctaag acaattcgcg gcacaagaat tccagaagta tttgactgct gggtagaatc
    17461 aggatcaatg ccatatgctt ctcgtcatta cccttttgaa aataaggaat tatttgatga
    17521 aacgtaccct gctcaatttg tcagtgagta catcgcccag actcgagcct ggttttacac
    17581 catgcatgtg atgtcggttg gaatctttga caaaatggcg gtagagaaca cccttactac
    17641 tggtactatt ctcgctgcag acggtagcaa gatgagtaaa tcaaagaaaa acttcccaga
    17701 tccaatggaa gtcatccaca aatacggggt tgatagcctt cgtctctacc tgatgagctc
    17761 gtcggttatg aaaggagaaa atagcaactt taacgaaaaa gaggtttccg atattcgcaa
    17821 gcgagttttt ctcatctggt ggaacatctt ttccttctac cgcttatatg cgcaaccaac
    17881 agatgttcca cccaaaccaa tcgccccccc acacatcatg gatcgctgga tactcagtcg
    17941 agccaactct ctaattcttc aagttacttc ctatttggat tcctacgatg tcgtccgagc
    18001 gagtcgggta cttattgaat ttgtaagtga actttcaacc tggtacatac gccaaagtcg
    18061 cgatcgtttc cgaaatcctg aaactagagc tgagagttgt caattacttg cctcggtttt
    18121 gattgcgtta tcaaaaaccg ctgcgccact aactcccttt ttcagtgaac taatctacca
    18181 aaatcttatc aataccagca ctagcctgca cttgacagat tggccagcta cgaagcagtc
    18241 ccttatcgat aatgagcttg aaaaagatat gcaactagtt caaaaaattt gtgaaaaagc
    18301 tcatgccatc aggaaagaaa aagccattaa ggttcgtcaa ccactggcaa ccttacaggt
    18361 aagatcatcc accaaccaac tcagcgatga gctgattgaa attctcaaac aagaattaaa
    18421 tgttaaatcg gttgattgga aaatgagtac tgaagaggtt gaagtagaac tcgatttaac
    18481 acttaatgca gatctaattg cagagggcca agctagagaa ataattcgat cgattcaggg
    18541 attgcgaaag gaggcggggc tgagcattga tgctgaaaca atagtcacgc taccaacctg
    18601 gccaaaaagc tggcagtctg aaattgaact aaaaacaaaa acgaaactta aacaaggacc
    18661 ttcactgcaa ataactacag agccactcga atgaaccgtt ggtttatacc cagcatcctc
    18721 ttggtcataa gcagcattag tgtggttttt attaactcat tagtgccgga attaacatcc
    18781 aaacaccttc tctcaatttt tcttggtttc gtagtttttg ttactactgc acgcttgcct
    18841 attcaacaag tattcaggcg tccctggcat ctctatctat ttacctgtat cctattgctg
    18901 attccattgt ttctcccggt tcaaattagg ggtacatcgc gatggattcc tatcattggg
    18961 tcgtttcgtc ttcaaccatc acaaatagct tttgcactaa taggaatttc gattagtagt
    19021 ttgacggtta agttttccaa agatacggcg caaaacataa aaagtactgc caaacagcta
    19081 ggcttgatta tcatacccac actattaatt gccgtggccc cgaacttagg aacagcagta
    19141 tttttctttt tttgtatgag tatgattatt tttttctcag gtataccctg gaaacatatt
    19201 gcattcttac tagctggagt aatttgcttc agttatatcg cctggttatt tcttttacag
    19261 ccgtatcaaa aacaaagaat cacaactttt ttgcagccaa gtcaagcaag ccaaaatgaa
    19321 acatataatg tgcgccaatc gctgattgcg attggcgccg gaggcctgtc tggaagtgga
    19381 tttggtaaag gtagccaatc acagctgaga tttttgccag agaaacatac tgattttgta
    19441 ttcgccgctt tctctgagga atttggattt attggctcag cattgttagt ccttatctat
    19501 ttggtgctga gcctctatct gcttaaagag agctatagca ttcagtcatt agaacaaaaa
    19561 tactttgttc ttttcaccag tacaattgta ttctttcaag cgatgatcaa catatctaca
    19621 aatatcggct tactcccagt aactggtata acattaccct tcttctcata tggaggcaca
    19681 tcatttattg catttggtct catatttgga acggctcaaa attgtataca gaaagaaaag
    19741 cgatattcac tccttcatat tgtgtagctt gccatgtcgt cgtataatac ccccctatgt
    19801 catacgcagt tgttcagctt caaggtaaac aatttattat cgaaccaggc actattcttg
    19861 aggtggatcg attagatgcc gaagaaaaaa aagcggtgga aatcaccgac gtgcttttac
    19921 ttgttacgga taaagaagtg cttgtgggca ctcccacagt cactcaggca aaaataaaaa
    19981 cagagtttgt taaggacctt cggggtgaaa aaatcagagt tgccaaatac aaagctaaat
    20041 ctagatatcg taaagtaaga ggccatcgcc aagatcttac tcaaattaaa gttgtgagca
    20101 ttgaggcgaa gtaaagtatc ttctcatgtc ttcaccagct gtccaacatt tttttctcct
    20161 tggttccact ccagaattat cttggctaga gcttagtcgt acatttcccg acatttgtgg
    20221 tttgatccga atttcaaatc aactcgccgg ttccagatta ctacctccgc aaatctctgc
    20281 taccaaagta ctcactttgc ttggaggagt tgtaaaaatt gctgaggtta tttccatgct
    20341 cccagatgat gaagaacaaa ttatttcaga aattgtaact tatttaaaac agggagaaag
    20401 ccgaattcat tttgctttag ctgagtgggg tagggatcat ttaccaaaag ttgaacccca
    20461 actaatcaag gagcgactaa ctcaagaagg caaatctgtt cgttaccgca caggatcaag
    20521 aactggttta tcggctgcta ttttattaca cgaatctgat ctacaagaat taattgttat
    20581 ctccacaaat gatggtgctt atcttgcaaa aaccagcgct gttcaaaata tcgaccaatg
    20641 gcaaattgag gacagatcca aaccatttgc ccaacataaa agaggattac tacctcccaa
    20701 agttggtcgc atgatggtgg caataggttt aggcaaaact atttttgata ctgacaacca
    20761 aaaaagtccg ctcgcactct gggatccctt ctgtggttcg ggcacaattc tttttgaagg
    20821 tctcttactc ggcctaccaa ttgtatatgg tagcgatatt gataccgaat caatcgaggg
    20881 tacggaaaag aattggcaat ggttcaaaaa tgctttccaa aatcagttag ctgataaaca
    20941 acaattagtc tcttttcaat ctgatgcaac gagtgttcaa actccaccgt ttgattcatc
    21001 aagtatctca atgattgtaa ctgagccatt tttgggaaaa ccaacccccg attttaggca
    21061 aattgcaaat attcagtctg gtcttgaaag aatgtacaaa ggtatgttta aagagtggaa
    21121 gcaatggcta aaacccggtg cttcagtcgt atgtgttttc cccaaatggc aaatttcagg
    21181 ggattacaaa caaaaaacgt tcgatttgca tcgattaatt gactttctca agccgatagg
    21241 ctacactact gcgtctgatc cagtcgtgta cgcaagacct ggagcagtag tccaaagaga
    21301 aatttggcat tttacgtatc aaaaccagta acctactatg tcacacgtta aagcatcagg
    21361 aaaagtcgct caacactcgc aacgaaaacg caaaggtctt cgccttggac ttaaaaaaag
    21421 cggaggacag gttgctgtag ctggcaacat tttaattcga caacgtggag cagtttatca
    21481 acccgctaaa ggggtaggaa tgggtaaaga ccacaccctc tattctttgg ttgatggcgt
    21541 ggttaccttc ggcaagcgcc gaggaaaaac aaccgtcagc gttctctaat tgattttttc
    21601 actttgattc agacatgcat ttctccataa gatatgtaat actaattgca tgtcaaaaga
    21661 tgtgtatcaa ctactcgata ttactttatt acagcccaat ccttttcaac caaggggcaa
    21721 gattcatgaa gaagaagtct ctgaactcgc cgaatctatc aagtcatacg gcattttaga
    21781 accaatcgta gtggcgcata ctccggctgg tcatcaaata attgctggtg agcggcgctg
    21841 gcgcgccgcc cagctagctg gcctcacaga agttccagtc atcatcagag aaactacacc
    21901 caaaggtatg ttagagatgg ccttgataga aaatgtccag agaataaacc ttaatgctat
    21961 tgagcgagcc aaagcttttc aacaattatt gcgagatttt cgttattctg cagttgaact
    22021 ggcaaataaa gttggtaagt cagaggcgtt tgtaagcaac tctctccgcc tactcaatct
    22081 gccagatgcc gtaaaagatg gtgttgttgg taaacagatt actgagggtc atgctcgtgc
    22141 tcttgccggt ctcgaggata aaaaattaat tattgaagta tataaaagac tattgaaaga
    22201 aaatgcgagt gtacgtcgag cagaggaact tgttagacag gtaaaacaag cgagcgaaac
    22261 aaaaaccacc actgctccag gcaaaagaat taaagtcgat ccagccttac tatctcgctg
    22321 gcaaaagagt tttgaagaat tcttgagtca ttcgtctcct agagtcaatt tgtctagatc
    22381 gacaaggcaa accagactaa cggtaacgct tagggggaat ctagaagaaa ctcaaaaaga
    22441 tttggagcaa atactacaat tagtgcaaaa acactaatag tttacgttct caatcatacc
    22501 tgcggctttt ggtggccaat acacaaaaaa ggctctgccc acaatttcat ctatagtaat
    22561 tgggccccaa gctcgagagt cactcgaata gggcctgtta tctccagaga caaagtattc
    22621 gttctgttcc aaaataatca ctttgtctcg agtgtattgg ccaggatagg tgtcataacc
    22681 agagggaata taggtttcct gcaacttggc attattcaca taaatcccat tgttctttac
    22741 ttcgacttgg tcgcccggta aaccaatgat tcgtttaata aaatcgcaac cagttccctc
    22801 ttggcaatgc gcctctgggg gagcgtgaaa aacaaccacc tcgcctcgct tgggagctct
    22861 aaatcgatag cttattttgt ctgtaagcac atattcaccg ctttgaaaat ttggcaccat
    22921 ggactgacca ttgacttgat gtggctgcat aaaaaaaaga tacactacca aaaacagtga
    22981 aaaaccaata accaaggttt ctgtcaggtc aataactaga ctaccaagtg aagcaaaaaa
    23041 tctcgacata acaatgtagt atgctttttt tcttcaattc ttgcaaccca caaactctca
    23101 gtaaaaaggc tataatactt tcatgggata ctcgggaaaa gtattttcag gttttagttg
    23161 gcaaactaca ctgagagtgg tgcttattgg ggtaggtttt gctcgtattt ttatcctcgc
    23221 aagacttctt actccgcaag acttcgggac cttcgcaatt gtcctaattt cacttggttt
    23281 acttgaggca atcacagaaa cgggcatcaa cctaacaata gtacaatcaa agcatcaaat
    23341 tgaatacttt attgatactg cctggattat ttcaattatt agaggtgcga tcatcagcac
    23401 actgatgatc gcttcaagtt tttttatagc actcttttat caaaaacccg agctagtgct
    23461 catgattatt ctcactgcgg ctgtacccct tataaaaggc ttcattaacc caactatagg
    23521 tgcattgcat aaaaatttcc aattttctac tcaagttttt tttcaattta gtcgatatgc
    23581 tgccgaagca attatcgcga tagtactggt attaatctgg ccaagtgtct ggtccttagt
    23641 aatagcactt attgttgcag ccctctttga gattgggatt tcgatggtat tttttcatcc
    23701 caaacccaaa tttcgctttc aaatggcaag ggctcggatc atactgaata attcaaaaac
    23761 tcttacacta caagcacttt tgtcatattt agtagaaaat gttgacgatc ttctcatcgg
    23821 aaagacactc ggaccacata tgttgggttt gtatcacaat ggttatgcgc tcacccataa
    23881 gcttctgtat gattctgcca aatctgccag ctatggtttg ctaccaacat atactaaact
    23941 gattgatgat ccagagagac ttaagcgtgg attattacgc tcaaccttat tttttagtag
    24001 tttgctcatt tgcggaacaa ttgtaattgc attgctagca agaccgattg tgctactcgc
    24061 attaggtgaa aaatggcaaa gtatcattcc aattattagt gttttggctt tcgccgcact
    24121 tttacaatct atttcaacac tactcagcac ttttttgtac gctacgaaaa aataccacgg
    24181 catcaccttt ggtttgctgg tccaagtgat aattttgatt tgctcactgt ggtaccttag
    24241 tatgcaattt ggattaatcg gtgcagcatg ggcaattgtg gtagctcgta tggcaggatt
    24301 accattttta atttgggctg taatcaaaaa ataaatatca atcatgaaaa aaattccact
    24361 cagcgtactg attcttgcca atcagctgtc accaaaactt atggcagcaa tacatagcgt
    24421 cacatttgct gatgaaatca ttattattga tacttctgga acaaaggcaa tttcgtttcc
    24481 aaaacaaatt tctgctattg ttaagcaagt caaaccagtt acaaactttg cggcaacaag
    24541 aaaccactca ctttcattcg cacataacaa ttgggttttt tttcttgact cagacgaggt
    24601 ttgtacctgt ccaaaacctg acttattaat tcccatgctc caaaaagatt gctctggctt
    24661 tcaagtcagt agagaggacc attttctggg aaaagtgctg catcatggag aaacagcaaa
    24721 atcctggcca acgaggataa taaacaaaac tgcctgtgcg tatcagagca tcgttcatga
    24781 aaaagtaatt tgtgagggca ctattcaatc gataaatcca gaaattctat acatcgagca
    24841 ctttcctcat gattcaatct atagtttttt taaaaaaatt agttggtaca gcaaacttga
    24901 agcagagcaa agaaatacgc ctaccatcag actgattttt gaacttttta cctatcccac
    24961 tggcaaatgg attgctaatt tattgtttga tcaaggactt ctggatggat accgaggttt
    25021 agtctacgcc actcttatga gttatcactc tctgctggta cgattatttc aactagaaaa
    25081 acagataatt aaaagacatc gtgattaatt ttcttatact tctactatta ccattggttt
    25141 tgctcggaca attgcttcgt tttgaacccg ttggtggctt aagtatcttt ccccacgatg
    25201 tcgtaattgg tgtaattctt ctgctcgttc tctgtgacaa agttcaaaga cgtctatttg
    25261 taccttttct gcgatttatc aaatctaact ggcttttttt cattctatgg acacttctag
    25321 cttgttgctt ccaaatagtg ttttttcaag aatctgttta tttgttactc tacattctgc
    25381 gtactggatt ctatctattt ttcctcagct ggttttttta ttacttacga aaatcagatt
    25441 tcaagaccat tgcgttctac tcattgagcg gggtattctt ttcgatcatt acactcggca
    25501 tgtatttgat ttttccagat ctaagagcat taaaaagcct cggctgggat gatcattatt
    25561 accgcgccgt tggcaccctg cttgatccaa atttcactgg aattattatt gtcgtttgtc
    25621 tgttaaattg ggttggctta ttcagcatca gaacaatacg gtcaataatt tttcagtatt
    25681 ctctgatcgg tttactaact atctctctag gtctgacatt ttctcgggct agttggttga
    25741 cgtatctttt ttgccttggt ctaatcctag ttccagtact gatcagactc aaatttcaat
    25801 ttttacaagc cacaatagtg aagttaattt tatttagcat agctttattg attattacaa
    25861 tcaccgtagc gccaaaaccc ggtggagcag gagttgattt gtccagaagt gcaactgttg
    25921 tcgcgcgcca acaacatgat caatcactgc tcgatactaa tcgagtacgc ttaataatcg
    25981 gtgggggatt gtcatcggct ccaaactccg aacttagata tcatgctcga ctaccaaaca
    26041 acctcggtat gattatatta gctttttccg gtattcccgg actagtaaca tttttctggg
    26101 gtttatcgca aacatttagg atgatcatta aacaatatcc actgatgtta atacctgcca
    26161 tcgcagtact tttctttgcg cagtttaatg cggcattgac cgaaccattt gtactgttga
    26221 tttgtgggat acaattgatt gtactgaccg cagaaaaaaa gctatttaaa ccttttgctt
    26281 aaagttacag cttaaaattt actcgcaatt cttgttcgaa aatgttattt gccttatctg
    26341 ttatcactag ctttagtaag ttttcaccct cattgagttg aatttggtag gaaaaactac
    26401 catctggtcg agcaaataaa aagcgatcat taattgccac tttcgcattt tgctccgtca
    26461 gtccagaaat tgtgaaagac ttgttttcct tgcccaccac ctgcatacca tcttgaattg
    26521 tttctgacaa tgctaatgag ggggcttcag tgtcaagcga aattacgtat tctttggaaa
    26581 gattagattc attccccgca gcgtcctttg agaaggcttg taaataattc tcaccttctt
    26641 gcagatctat gagttgctta aataccgcat catccgaagt ggtgaggtca gcgacttggt
    26701 ttccattgag cactatcaat acctggcttt ttgcttcggc aaatccactc acctcaattt
    26761 gtttggtact tgttgccatg ggaggtgagt tgaaaatagg gacttgtgga gggattgtat
    26821 cttcttgtcc aaataccgct ccaccatcca caatactcgc tagtaatcgt ggagcattgg
    26881 gaagaataat aaaaagaaat agtaataaca acccaagagc aatgaggctc caagtcattg
    26941 tttgttttaa caatttgtct cgttctcgtt taactattct tgaagtggtt ggagttggac
    27001 tcataggcga atcgtttcta tttactactc gatattctgt ttgtttcatc tgagccgaga
    27061 gggggattcg aacccccgac ctacggttta cgataccgcc gctctaccaa ctgagctatc
    27121 tcggcgc
//
LOCUS       DAOQZP010000006        24684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_2355_length_24684_cov_5.253319, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000006 DAOQZP010000000
VERSION     DAOQZP010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 24684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24684
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2355_length_24684_cov_5.253319"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="PKH60_00650"
     CDS             complement(<1..1089)
                     /locus_tag="PKH60_00650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNQ16591.1"
                     /translation="MKNNIILLIIIGVAAILRLCGLGTIPIELNRDEASIGYTSYSLL
                     KTGADEYGRKWPLNLESFGDWKLPVYEWATIPSIALFDLSAWSVRLPSAIAGIVAVYL
                     LYQLTLIVIAQSKYRYSFALLTAVFLAISPWHIHFSRMAYEANLGLTLFLAGLLLFLT
                     YINKKRASVLLLSGICFGLTMICYHAFQVVTPLIIIFLMIIFRKHIAFKLQKQLFSWV
                     FFIGLVLMPVFLLFASGLTHSNQVKLSGLSIFEEQTYYLRLFNKREYFNENQAILAKL
                     YTNIPMEFFRQLFLNSTQALNPDFLFINGAGHGSHDVVGIGKFYSVMLPLLILGLATL
                     FSRHSLFTSNGRWLVIAWLVVGLLPAIIT"
     gene            complement(1079..2782)
                     /locus_tag="PKH60_00655"
     CDS             complement(1079..2782)
                     /locus_tag="PKH60_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16592.1"
                     /translation="MRIFTIATIVILAVVFRFFQLGNLPPSPYWEEAALGYDAYSILK
                     TGKDHHGNWFPIVAFESFGDWKPGFYIYTVVPSIFIFGLNTFAVRFPSAVAGLAIVIG
                     SGILAKELFLEKKQPRLINEKSRHWLPYLVMLVTAVEPWGIIFSRAAWEANLATALIV
                     WSIIFVFKQSRSTNKYIPSYLIAAAIGTVLAMYTYHAARIAAPMMVGAAFLFTELEQL
                     AIWSKVKTRSKRLRFLLLPAVTFVLMLSPLLLNMTSNTVTNRFATTSIFSDLDVIIQS
                     NQLKELSDNSFLSRIVYHRYLLFGKEIITNFGKHFDLGYLFLHGDTNPRHSIQYVGQL
                     YPLSLVLLIIGLSILVYRHRRQAVLFVFWFLFGLFASTLVVGSPHAVRSLVVFPFFTI
                     IIGLGLHRTIDWLSRIGKPKQMIFTFVFSIIFVAQIGVFWLYYSQVYPAVYAQEWQFG
                     YQEMIATVNQLALAYPDEQIYITREQGRPAMYYWFYSKTDPKLAQSYEASAQKDQSEF
                     LQFGRFHFIDKVDDVSTSYGILAASPGFTADVGLPTEGDVAIVSNLLGQPIWIVQHYE
                     K"
     gene            2786..4333
                     /locus_tag="PKH60_00660"
     CDS             2786..4333
                     /locus_tag="PKH60_00660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNQ16593.1"
                     /translation="MYHSKLKTGNTLAVFFLLFFSLIFRFVFSATHISFGQDVARDAW
                     LIEQRINQGNLIVDYGPKASVGNFYLPPLYYQLHVFFSLLTNNDPLTMKWVITFVEAL
                     SPVLLFLILRQIFDSKLALLTGLLYIIAPIPLQYGTTAWNPNMIPFFSLLFLYSGIQI
                     MKYQKFWFIPVFCLACITAFQFHYQSAVLVLFFVFIALYSIQKFTLKRIFSYWVVGLL
                     VIILTLLPYGIAEYRSQFKNTKAIIDYFSSEHSQYFERVSKPAYVLTYFPKFFDRVML
                     SKETYHSIIGICLYFGGSLVLLIRYRKTKNIANLFILFYFFSIVVMLRVFKGDKLDYY
                     LSTLYFIPFILLASVLSITKSNVVTGSIFGLMFLSLGIHVSQWYKYDDLASLKESVAY
                     LQSTVPTQNVRFIFHDDDFVNSMYFGVIKYSTLTLDKNSSTIIDICNNRDVCVWDTRP
                     ASKHSLAYSKVGFFKSDSQYQQLSYRYGKVPFQIIIGSIKNPSVLLDTTGFNYQGERG
                     SDFLMEY"
     gene            complement(4337..5968)
                     /locus_tag="PKH60_00665"
     CDS             complement(4337..5968)
                     /locus_tag="PKH60_00665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNQ16594.1"
                     /translation="MATVLDTKKTPIMKIAGKLPLLFIVLIAGLLRFWQLGNIPAGPN
                     WDEAAIGYNGWSVWQTRRDEWLERLPISFRSFGDYKAPLAIYANGLFTSLFGLNVWVI
                     RLPFALSGVLGVVCFYYLISNLLALKQKQKSYSSSKWLAVIGSATLAISPWHILFSRV
                     GFESGLALTEVILATALLILSVQKKWPRWQVLGLQIASGLIFASTFYTYHSAKVMVPI
                     FLVFLLLLLQRSKLLSIKHGFLYLSGIIVAIIPFILDSLLGEGLARASVTIFAQTLSF
                     PDLITTVVQNTLAHLNPKFLVMGQADSIRHSIGSMGVLFPSTAFLAIIGFIFSLRGRR
                     TFIWQLSLIWIVAGLVPAIIGVEVPHPNRALLALPGFLLLALLGLDHLTKIIFSFVKP
                     NRKIQIALICFLVVVEITQLSLFLHQYFGTYNRQSSSSFQDGYSEMFAEVWSYYDGNN
                     GKHSVDQIVITSEYGQPYIYALLTRKISTFQYHNGALINFLFPDVVQLSDLDRPNALV
                     VAGEKSDLIDRTKAIKVIYANDGQPRFWLFDTNLL"
     gene            complement(5929..6831)
                     /locus_tag="PKH60_00670"
     CDS             complement(5929..6831)
                     /locus_tag="PKH60_00670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ16595.1"
                     /translation="MSNSFSVSTIIPTFNGRKLLETHLPSILRSLRDGDELIIIDDAS
                     QDESGNWLKYGFSLTQQKNPNLTDKHGAFELYQGFTNLKNKKITISLLVNEKNLRFAA
                     SVNRAVRFATKPLLFLLNSDVSLRPDTIKCLVTAYQTNAQPIFAIGCLEFEGSEADDL
                     ATLPTAGKNKLWFERGYFMHSKLNTDASGETAWASGGSALFNRERWLSLGGFDEHFAP
                     AYWEDIDLSYRARLRNWQVLFEKSAIVFHQHESTHQNVFSSQAIKNISWQHAAYFVQK
                     HGNFWQKLAYLLWQPYWILKKRQS"
     gene            complement(6824..7936)
                     /locus_tag="PKH60_00675"
     CDS             complement(6824..7936)
                     /locus_tag="PKH60_00675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ16596.1"
                     /translation="MKAVFVSPYLKQIHTGGGEKHLFDVALSLPKKIELNFALPTESD
                     ISDDSFRTSVENYRNRYQVFLGKSLQRIKFIRTPLFTQAPWYKKLLWTQNFDYIYYVT
                     DGSLFFSLARNNLLHIQTPLKNNYPNFLQSIKIKQWHPVNTNSQFTKAIVESNWGISV
                     DQVLYPEIDESLLASKANKNPIILSVGRFFPQLHSKRQDVLVRGFKQLLKEQPRLLAG
                     WELVFVGSIENQAFYDRVVAMAKGLPIRFETKLDHTELNELYSQARIFWHATGYEVDQ
                     DQSPEKVEHFGIATVEAMAAGAIPLVIASGGQIEIIPQELVSYCWTTIPELVSNTIQL
                     LRDPAKETQLRKIVRHRAKHFSHQELESTVRKMFNV"
     gene            complement(7942..8760)
                     /locus_tag="PKH60_00680"
     CDS             complement(7942..8760)
                     /locus_tag="PKH60_00680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ16597.1"
                     /translation="MKKAAVTAIILARNEAEMLPGCLRTIAWCKEIIVIDDGSSDNTS
                     EIAENAGCKVISFKHQSFARRREEGAKFAAHDWILYIDADERIIPELADEMQRVVSES
                     NNSAFQMNRTNIFFGKVFQYGGWETDTVTRLIDKRKLKGWKGDIHESPIISGTVGTLK
                     TNLLHFSHRTIVGGLYKTAAWTPVEAQLLAKASTTKISFAIIIKKGLGEFWRRAIKYR
                     GYHDGEPGLMESLIQAINRMLVYLQIWELQQTPPIKELYAVKEREVEILWEQHK"
     gene            complement(8757..9674)
                     /locus_tag="PKH60_00685"
     CDS             complement(8757..9674)
                     /locus_tag="PKH60_00685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ16598.1"
                     /translation="MKHVSVILVHYNNTKTTNSCLSSLSSLIHPGYACQYIIVDNGSI
                     KKYVLPKSLTKSKKFKVIRSDANLGFTGGNNLGIHYAVERNNSQFIFLLNNDTTIHKQ
                     ALRNLLQTLEDQPEVGIICPKIYFSAGKEYHSKSYLPSERGEVFWYAGGSIDWQHIVA
                     FHRGVDEVDRGQFNNAAATDFATGCAMLIRREVLEKVGFLDKRYFMYFEDVDLSLRAK
                     KAGYTIAFCPNAIVWHDNAGSSGGSGSSLHVYYQERNRYLLAFAHGTPAVIPVVLRLQ
                     FQALTSGSRLRRKAVLDFYKGSFGKQSLV"
     gene            complement(9689..10594)
                     /locus_tag="PKH60_00690"
     CDS             complement(9689..10594)
                     /locus_tag="PKH60_00690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ16599.1"
                     /translation="MSQITAIVTTHNAAKTLSDCLKSLSFVDEIIVLDMESTDETRGI
                     ARKFHTKVISIPPAEYVELVRNTSVKEGTKPWILLVDPDETIPDQLQQKLLSLVDDTH
                     AQISAYKIARKNLVFGTWIQHSGWWPDYQIRFFKRGAVSWSGVIHEQPSVNGAVGVID
                     ASSELAIEHTNYSSLDEYFQRMMRYTQAEVGDKSSKSLTKINSDQLFLAFFNDFFRRY
                     YQDEGYLDGLHGTSLALLQSLYQVTTALRIWEKQQFPYSANTVTDSTINQVISDWKYW
                     QASKMSLQTKGLKKAFWLLRKKLRF"
     gene            complement(10597..12063)
                     /locus_tag="PKH60_00695"
     CDS             complement(10597..12063)
                     /locus_tag="PKH60_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNQ16600.1"
                     /translation="MQKTTTNDELQIIKRRSISGAMSYLLRTIILQGIGFLSLAILSS
                     LFAPEDFGIYGFILMIIGLLTFFSDVGLAAALIQKKTDPTVEEYRTAFTIQQLLGWFI
                     VVIAVLLVASGIVSRKTGWAGDWILLTLAISFPLTTLKTIPSVILERKLEFSKLVIPQ
                     IAEQIVFHGVLIFMAFQQWGVISYAFAIGARTIIGILLMNVIQQWQFGLAITKPAIKT
                     LLSYGVKFQLNDFLARIKDQLFYIVVGVMMPLREFGYIQWSKNWSMYPYTLTVQNVMA
                     ITFPTFSRLQHRKDALAKGLEISLFWITLAIFPIIVGMASFITPFLSVFSSTYGKWMP
                     AAFSFALFSLSIGWSAISTPLTNLLNAMGKINQTLKLMIMWTLLTWVLTPPLVWWFGY
                     NGVAIAALLISFSSIAAVWMVQKELSFKLWDQIWPQLAAATVMAAVALIGRTYWQQSV
                     GFLVSGMFVALLSYVLTVFVLAKDRLIRNTLYLLRERA"
     gene            complement(12067..13743)
                     /locus_tag="PKH60_00700"
     CDS             complement(12067..13743)
                     /locus_tag="PKH60_00700"
                     /inference="COORDINATES: protein motif:HMM:NF021635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HNQ16601.1"
                     /translation="MLKRVLVFLMLLVILFISSRSFLLGKLFFLHDFTHAARIAEMSR
                     ALQDGDFPVQWSKNFGYGYGMPLFLFYGPFPYYIGAFLYIVSDSISLSLLLLFISVNI
                     VTILGSYRLGKEISGNAGGVIFAALYTLAPYRAVNLFLRGAINELWGMALLPWLVLGW
                     YLMIKKHRLGWLYISLSTAIIILSHNLSALYFVPLGFLSGLLLYLWKRKNIKRSIQEM
                     LSLLVKGLLASLLGFGLSAFYALPALFEKSFTRLDEQILTGYFDYHNHFLYLRQFLLD
                     NWKYGGSEWGPNDGINFFLGYGVLFGLGLAVFHALKNFKRVSKQTSLSIFLLFMTMGG
                     LILLTTQKSLWLWENLPMIAYLQFPWRLLSAIVFIATVIIAMFFSNNFKRKRAAVLTI
                     VTITLITSFRYFVPAAYYDSNNGPYYTDATRIQTEMSGILPDYIPKTFNTDIEPFQQL
                     VKESELLTVTILKDATSQKILLVKAKEPVPITISIANFPGWKAYLDGAEIQILTSENG
                     LIEVNLPEGEHLLNLNFGATKVRMWSYLISTIAVILLFVKLFSTQTKKQN"
     gene            13785..14870
                     /locus_tag="PKH60_00705"
     CDS             13785..14870
                     /locus_tag="PKH60_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNQ16602.1"
                     /translation="MNRNLIKLMLKAQLATAGFLLVLFVFTGLILGLIGWYQFSLFQK
                     SSGLTTTEILRMAKNAWQNPFNHSNSRISILVLGLDEVEARSQTVLTDTIILLTMNLE
                     TGEINSLSLPRDLWIDEYQTKINSLYYYGQKANPEQPTNLVTSAIESLSGISIDRIIP
                     VNLEQLAELIDTVGGLEITVAQAFTDDHFPIPGLNPSQITDPTLLQQTVSFEAGTQTM
                     NGNRVLEYIRSRQAIGENGSDDSRTRRQQQVIVALLDKLQSKSVLTNPELLGRLVNWY
                     RRNYESYWPLSEILQIGVQFVKNDLRPKITTHTLPIAENYGEYGVLIHPPIWRYQNQW
                     VYIATDSAILKEYIQSSFDLNSPPTPQ"
     gene            14875..15093
                     /locus_tag="PKH60_00710"
     CDS             14875..15093
                     /locus_tag="PKH60_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16603.1"
                     /translation="MKLPNIALPSQKTITRLLFLSTIIFALLTGLYQELYRIEQKKYA
                     NLENMYVRVRSQLGRTETQRLIDESYNK"
     gene            15107..16150
                     /gene="rfbB"
                     /locus_tag="PKH60_00715"
     CDS             15107..16150
                     /gene="rfbB"
                     /locus_tag="PKH60_00715"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006171302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNQ16604.1"
                     /translation="MNKTLDKHNTLVVTGGAGFIGTNFCYYWNKQHPDDRIIILDALT
                     YAGNRSSLAELEKQSTVRFVHGTINDAALVDDILSEATMVIHFAAESHVDRSITNPNL
                     FLQTNVMGTHTLLQAALKHSVARFHHISTDEVFGSLPLDSREGFSETTAYSPRSPYAA
                     SKAASDHLVRAYAETYGLPVTITNCSNNYGEYQFPEKLIPLAITNLLEGGTVPIYGPG
                     NQIRDWLYVQDHCRAIELVLLNGEVGQTYLVGGLTEDITNLELIKLVLKIMNRSESSI
                     QFVADRPGHDQKYRVNWNTIQSQLGWQPSVDLEQGLQRTIDWYTNNQAWWKPLKEANA
                     AYFSAQYNTAESS"
     gene            16153..16764
                     /locus_tag="PKH60_00720"
     CDS             16153..16764
                     /locus_tag="PKH60_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HNQ16605.1"
                     /translation="MPSDNFVPTESNHVVDGIYQTAIEGLLFSASQKHIDHRGHYAEL
                     VRLPDLNMALKQPFVPVQINHSHSHLNVIRGFHAENWNKLVTIVHGYCYCVLVDVRPN
                     SATFGHSVSFNLGNHDDALYGSLYIPAGVANSALALSEPVDYLYFVDALYANRDRTGD
                     VSISLFDKDINARWPIAQKTMIVSERDRQSVTLREKFPNKFPS"
     gene            16782..17693
                     /locus_tag="PKH60_00725"
     CDS             16782..17693
                     /locus_tag="PKH60_00725"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ16606.1"
                     /translation="MNNTKKPSIIGTGLNGLVGSKIVKDLSAVLQFENIDISDKTNPV
                     DITNIQQVESFIGNSAASVVLHCAAFTNVTAAWEQTGNKSGLAYAINVVGTRNIVNAC
                     KKYSKFLIHLSTAYVFDGTKDELYTEFDTPHPIEWYGQTKLWAEEEVTNSIDQAAILR
                     IDQPFRSDSYPRADILHRILNQLTDDSLPPQFTDHSFGPTYIDDLAKTILWFTQTKTA
                     GIFHATNGERWNDYDFARKVAEAAQLSTIVKPGSLAKYLTTSKRPYQKNTALDTTKLL
                     QVIPHKPTPISVAIREACTYFESNESK"
     gene            complement(17666..18325)
                     /gene="trmD"
                     /locus_tag="PKH60_00730"
     CDS             complement(17666..18325)
                     /gene="trmD"
                     /locus_tag="PKH60_00730"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNQ16607.1"
                     /translation="MIFTILTTFPQYFESPLDSSIIHRAISNKIIDVNLIDLRQFTND
                     KHKTTDDRPFGGGAGMVMKIEPIEKALASIPISQPKKNRTILLSARGNRFVQTDASRL
                     ATYQSVSLICGHYGDVDQRVADYLVDEEISLGDFVMTGGEPAALVMIDAISRLLPNVL
                     GNEASIVAESHAQPGISSPPSYTRPADYRGWKVPEVLLSGNAAAIKAWKADYLDSLDS
                     K"
     gene            18390..19481
                     /locus_tag="PKH60_00735"
     CDS             18390..19481
                     /locus_tag="PKH60_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16608.1"
                     /translation="MPATSNSGLTLTQAKTFSKSFVLYGSVGLVVFLVFWFASGAFIR
                     YWIATHPEPPPPPTVGFGILPAPQFPITPDESKPKQYILETPTGRLPDFSDRALVFFM
                     PKNSPGLLDAERSKNFASEYEFLSAPEILDSRTYLWQKTQPLFTTLEYDIQDQVFSYV
                     SDFSNRPELLLDSELPTKFDAINEVKSFIKKAVDLPSDISTASGTFAFVKSVGSDLLP
                     AVSVTDANFVTVDLNRSPIESRYQIYTDRDGRGVIHALVGNIKNTEKILQLDYYYYPI
                     DYSVIHSYPLRPVKQAWDLLQGGEGYISHAVSSDQATIREVTLGYYDSTTPQMYLQPI
                     YVFSGDDGFLGFVPAIDPSFLNTTKTNLP"
     gene            19478..20914
                     /gene="gltX"
                     /locus_tag="PKH60_00740"
     CDS             19478..20914
                     /gene="gltX"
                     /locus_tag="PKH60_00740"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNQ16609.1"
                     /translation="MKVESIRTRVAPSPTGLPHIGTVFQALLDYIIAKQNKGTFVLRI
                     EDTDQKRLDPQAEQAVYEALEWVNIQPDESPKAGGAFGPYRQSERLLLYHKYAQQLVD
                     QGAAYYCFCSAERLESVRNQMQKDGKPPMYDKLCRSISPSEAKERAAAEPHVIRLKVP
                     TQTQISVLDAVRGTIVFNSDDIDDQVLLKSDGFPTYHLAVVVDDHLMEISHVVRGEEW
                     LSSAPKHILLYDYFNWQKPVFFHTPTLRNPDKSKLSKRHGHTTVEWYRSEGIVPEALI
                     NFLLSIVWTHPDQSDIFSLQEAIELFKFQSVHITGPIVDLAKLRWINGQYIRAYSKND
                     FVNLAQPFLPADFPKEKAASILPLIHERLEQLNQIESLTDFFYRPIVLDEKPLLQRST
                     AEVVINQLTGTIDRLEALPEWNLHQIEDAIRSLQEKNDWQRKQYFMLLRYSTTGKSAT
                     PPLFETIEVLGKTLTIDRLISARTLITS"
     gene            20917..21654
                     /locus_tag="PKH60_00745"
     CDS             20917..21654
                     /locus_tag="PKH60_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16610.1"
                     /translation="MFQQQDLTQQADLAYEHLLQVFPLAKKFHPSLHQRISVDQSLKQ
                     PLQEFNKSEKSIIFDKLRQTHKQPLSLVPSETLLYLEQQLGDIFGLALTSETNLGSLP
                     YLVGQIEAQPHLKSSPTDTINNHSSILEAGLRPERSYYGWQSEPYGISLPLHELAGWQ
                     TLSDKERKAYLSQLTLVINPWLEIAIVAKIVDIGPYHTQRYQFAGSPQLVREGKFWNP
                     GYNGLAAIFFLESNSTHLKTGPLSYER"
     gene            21644..21925
                     /locus_tag="PKH60_00750"
     CDS             21644..21925
                     /locus_tag="PKH60_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16611.1"
                     /translation="MNAKVIQSLTAIVPTLRWSDDVTIQHLQLSNSLTVIAEQLSPNQ
                     QAELAEMILDADTPPNKIAKWLTDQIPQITLLLRELESKVTLITRNMQP"
     gene            21991..22320
                     /gene="ybeY"
                     /locus_tag="PKH60_00755"
     CDS             21991..22320
                     /gene="ybeY"
                     /locus_tag="PKH60_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNQ16612.1"
                     /translation="MKILNEYKIENAQVDISVVGVRKITELNENRLGHDGSTDVLSFP
                     LHEKKALDDFPLPNTIPPHLGDIVISFPAALEQAKRFGRLVDDQICFFVEHGMLHLLG
                     YHHEDEN"
     gene            22310..23860
                     /locus_tag="PKH60_00760"
     CDS             22310..23860
                     /locus_tag="PKH60_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ16613.1"
                     /translation="MKTNQSLAWTTSYRPISLSQLHQVSVRNQLIQYQNEGSFPQVLL
                     FAGPKGTGKTTSARIIAALLNDPKNTKIVTNNYIQHQSEKKALSDPDVSNELIAKILT
                     GTSFTVHELDAASNRGINEMRAIKDQAQLSPIEGIISVFILDEVHMLTAPAFNALLKL
                     LEEPPSHTTFILATTELQKIPETVVSRCQVVHFHQASQAEIQSALEFVTQSESLQINA
                     DVLARVADLANGSFRDAVKYLQKIATYKNPTLDLVEALIAPSFSARIDSIITALLDKD
                     SAMICKLFVDLRSEVYDEKLFITQLLETIHQDLLLHYLPELDSQPVRTVAINLFLLKE
                     FTQSISYPPNPIPFLSLEITCLNIIERSQQKTKKGSGDNGTTVTKKITPQRIEVNNSS
                     NSKGDAQKLLENWQRFLTEVANHNTTLAALFRSAQPKRANDHSLEIEVYYQFHKEQLI
                     NLKHHAAIQPVLNTLVGGDVHFDISVVTAPVAAELLDEVPTSLPERVAAALLQSDPHS
                     ATDVVQSN"
     gene            23881..24168
                     /locus_tag="PKH60_00765"
     CDS             23881..24168
                     /locus_tag="PKH60_00765"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNQ16614.1"
                     /translation="MQNPLKALGDLNSLRQQAKQMQDELGKEEIVIEKGDIRVVITGD
                     QKIKQFSVQGISSEDAIAVLNEAIKKSQELAAKKLQSMTGGLGGLLGKMGQ"
     gene            24169..>24684
                     /gene="rdgB"
                     /locus_tag="PKH60_00770"
     CDS             24169..>24684
                     /gene="rdgB"
                     /locus_tag="PKH60_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNQ16615.1"
                     /translation="MSPRLLVASTNQGKLTEIKGILSDLGLDIVNLSEFPTLVVSSVE
                     ETGITFAENAAIKAKHYASITQLPTLAEDSGLIITALNGEPGVQSARWFPGSDEARSQ
                     AILSRMKTISNRSAFFHCSVCLYHPLTRVTQTFDGEVEGTIAQECRGEDGFGYDPIFI
                     PNGYSETFAELG"
BASE COUNT         7702 a         5089 c         4707 g         7186 t
ORIGIN      
        1 agtaataatg gctggcaata aaccaacgac tagccaagca ataactaacc acctgccatt
       61 agaggtaaat aaagaatgac gagaaaacaa agtggcaagg cctagtatga gtaacggcaa
      121 catgacgcta taaaactttc cgattccaac aacatcgtga gaaccatgac cggcgccatt
      181 gataaaaaga aagtctggat ttaatgcctg agtgctattt aggaaaagct gtcgaaaaaa
      241 ctccatagga atgttggtgt aaagttttgc caatatagct tgattctcgt tgaaatattc
      301 tcgtttgttg aataggcgca aataatacgt ttgctcttca aatattgaaa ggcctgaaag
      361 cttaacttga ttactgtggg ttaatccaga agcaaataat aaaaacacag gcatcaaaac
      421 taatccgata aagaacaccc agctaaacaa ctgtttttgt agtttaaacg caatgtgttt
      481 tcgaaagatg atcattaaaa atataataat taagggagta actacttgaa atgcatggta
      541 gcaaatcatc gttaaaccaa agcatattcc actcaacagt aatactgatg ctcttttttt
      601 gtttatatac gtgagaaata ggagtaagcc ggctagaaag agggttaaac ccagatttgc
      661 ttcataagcc atacgagaaa aatgaatatg ccaaggcgaa atagctaaaa atactgcagt
      721 tagtaaagcg aaggaatatc tgtattttga ttgagcaata acaatcaatg tgagttgata
      781 gagtaaatac acggcaacta ttccggctat ggctgaaggc aatcgaacag accaagcgga
      841 taaatcaaac agggcaatac tgggaatagt tgcccactca taaactggga gtttccagtc
      901 accaaacgac tctaggttga gcggccattt tctgccatat tcatctgcac ctgtttttag
      961 taacgaatat gaagtgtaac caattgatgc ttcgtctcga tttagctcaa ttggtatggt
     1021 accaagtcca cataatctga gtatcgctgc aacaccgatg ataataagta aaattatatt
     1081 atttttcata gtgttgaacg atccagatag gttgtcccag taagtttgaa acaattgcca
     1141 catctccttc cgtaggtaaa ccaacatctg cagtaaaccc aggtgaggca gcgaggatgc
     1201 cgtaactagt tgaaacatca tcgactttat ctatgaaatg aaatcgacca aattgtaaga
     1261 attccgattg atccttctgc gccgaggctt cgtatgattg cgctagtttt ggatcagttt
     1321 ttgaataaaa ccaataatac atagcaggtc tgccctgctc tctagtgatg tagatttgct
     1381 cgtccggata tgctaaggcc agctgattga cagtagcaat catttcttgg taaccaaact
     1441 gccattcttg agcgtaaacc gcaggatata cctgactgta atagagccaa aatacaccta
     1501 tttgtgccac aaagatgatt gaaaagacga atgtaaaaat catttgcttt ggtttaccaa
     1561 ttcgggaaag ccaatcaatt gttcgatgta aacccaatcc aataatgata gtaaaaaaag
     1621 gaaatactac taatgagcgt actgcgtggg gtgaaccaac aacgagcgtc gaggcgaaaa
     1681 gaccgaatag gaaccaaaat acaaatagaa ctgcttgccg tcgatggcga taaaccaata
     1741 tggataaacc aataatgagc aatactaaac tcagaggata cagttgacct acatattgga
     1801 ttgaatgacg tggatttgtg tcaccgtgga gaaacaaata ccctaaatcg aagtgtttac
     1861 caaaattagt aattatctct ttaccaaata gtaggtaacg atggtacaca attctagaaa
     1921 gaaaagagtt gtccgatagt tcttttagtt gattgctttg aatgatgacg tcaagatcgg
     1981 aaaatattga ggtagtggca aaccgattgg tgacggtatt tgatgtcatg ttgagcaaca
     2041 acggtgacaa cataagcaca aatgttactg ctggtagcaa taagaatctc aggcgcttgc
     2101 ttcttgtttt tactttggac caaattgcga gctgctccag ctcggtaaat aaaaatgccg
     2161 ccccaaccat cattggggcg gctattcttg ctgcatgata ggtatacatc gctaaaaccg
     2221 tcccaatagc tgccgcaatt aaatagctgg gaatatattt gtttgttgat cgagattgtt
     2281 taaatacaaa aataattgac catacaatta aagcagttgc taagttagct tcccaggcgg
     2341 ctctagaaaa aataattccc catggctcaa ctgccgtaac caacattaca agatacggca
     2401 gccaatgtct tgatttttcg ttaataagtc tcggctgttt tttctctagg aataattctt
     2461 ttgccaagat gcccgagcca ataacaattg ctaagccggc taccgcagaa ggaaaacgaa
     2521 cagcaaaggt atttaaacca aaaataaaga tgcttggtac cacggtataa atataaaatc
     2581 caggtttcca gtccccaaat gattcaaacg ccacaatagg aaaccaattc ccatgatgat
     2641 ccttgccagt tttaagaatt gagtaggcgt cgtagccaag agctgcttcc tcccaatatg
     2701 gcgaaggtgg taaatttccc aactggaaga aacgaaaaac gactgctagg ataactatag
     2761 tggcaatcgt aaaaatacgc atgatatgta tcatagcaag ctcaagaccg ggaatacact
     2821 agcagtcttt tttttactct tttttagttt gatttttcgt tttgtattct ctgcgactca
     2881 catttcattt ggtcaagatg ttgccagaga tgcttggcta attgagcaaa gaatcaatca
     2941 aggcaacctc attgtcgatt acggcccaaa ggcgagtgta ggaaattttt atttgccccc
     3001 actctattac caactacatg tattcttttc acttttaact aataatgatc cactaaccat
     3061 gaagtgggtg attacttttg tcgaggcgct gagtccagtc cttttatttc tgattttacg
     3121 gcaaatattt gatagcaaac tcgctttgtt gactggccta ctctacatca ttgcaccgat
     3181 accattgcag tacggaacta cagcctggaa tcccaacatg attccgtttt tttcattact
     3241 atttctgtac tctgggattc aaatcatgaa atatcagaag ttttggttca ttcccgtatt
     3301 ttgtttagcg tgtatcaccg cttttcaatt tcattatcaa tcagcggtgc ttgtgttgtt
     3361 ttttgtcttt attgcccttt actcaataca aaagtttacc ctaaaaagaa tatttagcta
     3421 ctgggtggtt gggctactag tcattattct cacgctatta ccttacggaa tagcagaata
     3481 tcgtagtcag tttaaaaata caaaagctat tattgattat ttttccagtg agcattcgca
     3541 atattttgaa cgagtatcca agccagcata cgtactaacc tattttccta aattctttga
     3601 tcgagtcatg ctgagtaaag aaacatacca ttcaatcatc ggaatctgtt tatattttgg
     3661 tggctctttg gttttactta tacggtatcg aaaaacgaaa aacattgcta acttatttat
     3721 ccttttttac tttttcagca tagtagtaat gctacgagtt tttaagggtg ataaattgga
     3781 ctactatttg agcacgttat atttcatacc gtttatcttg ctagctagtg ttctttctat
     3841 tacaaaatca aatgtagtga ctggaagtat ttttggtctg atgttcctct ctttgggaat
     3901 tcatgtaagt caatggtaca aatatgatga tctggcatca ctgaaagaat ctgtggctta
     3961 tcttcagtct acagttccca ctcaaaacgt gcgttttatt tttcacgatg atgattttgt
     4021 taactcaatg tattttggcg taatcaagta ttccactctt acactagaca agaattcgtc
     4081 aacaataatt gatatttgta ataatcgcga tgtctgtgtt tgggatacaa ggcctgcatc
     4141 caaacattcg cttgcatatt ctaaggttgg ttttttcaag tcagattctc aataccaaca
     4201 actcagttat cgatatggta aagtgccatt ccaaatcatc atcggttcga ttaaaaatcc
     4261 cagtgtgctt ctcgatacca caggtttcaa ttatcagggt gaacgaggaa gcgactttct
     4321 aatggagtat tgaattttat aagagattcg tatcaaaaag ccagaaacga ggttggccat
     4381 cgtttgcata aataactttt atcgcttttg ttctgtctat cagatcgctt ttttcacccg
     4441 ccactaccag tgcgtttggt ctatctaaat cactcagctg aaccacatcg ggaaataaaa
     4501 aattaattaa tgccccattg tgatattgaa aagttgaaat cttacgtgtg agtaaggcat
     4561 aaatatacgg ttgtccatat tcgctggtga tgacaatttg gtcaactgaa tgttttccat
     4621 tgttcccatc atagtatgac caaacttcgg caaacatttc tgaatagcca tcttgaaaag
     4681 atgagcttga ttgtctgtta tatgtaccaa agtattggtg taaaaataga gataattgag
     4741 ttatctcaac gacgactaag aaacaaataa gtgctatttg tatttttcga tttggtttga
     4801 caaacgaaaa aataattttg gttagatgat ccaaacccaa caatgcgagc agtaaaaatc
     4861 caggtagagc caaaagtgcc cggttgggat gtggtacttc gacgccaata atggctggaa
     4921 ctaatccggc tactatccaa attaaactaa gctgccaaat aaaagttcgc ctacccctca
     4981 gactaaaaat gaacccaatg attgctagaa aagcggttga tgggaaaagc actcccatcg
     5041 aacctattga gtgtctaatc gaatccgcct gacccataac tagaaatttc ggattgagat
     5101 gagctagagt attttgaaca acggttgtga ttaaatcagg aaaagagagt gtttgggcaa
     5161 aaatagtcac gcttgccctt gctaagcctt ctccaagtaa tgaatcaagt ataaacggaa
     5221 taattgccac gattattcca gacaggtaca gaaagccgtg tttaattgag agcaatttcg
     5281 atctctgaag tagcaacaac agaaaaacca aaaagattgg caccatcact ttggcgctat
     5341 gataggtata aaatgtactg gcaaagatca atcctgatgc gatttgcaag cctaagactt
     5401 gccagcgagg ccattttttt tgaacagaca gtataagaag agccgtggcc aaaattactt
     5461 ccgttaaagc gagtccgctc tcaaaaccca ctcttgagaa gagaatgtgc catggagaaa
     5521 tagcgagtgt tgcagaccca attactgcca accattttga gctactgtac gatttctgtt
     5581 tttgcttaag tgcaagtagg ttagaaatta aatagtagaa acaaaccaca cctaacactc
     5641 cagatagagc aaatggtaag cgaataaccc agacatttaa accgaacagc gaggtaaata
     5701 gaccatttgc atagattgcc aatggcgctt tgtaatcacc aaatgaacga aatgatattg
     5761 gcaatcgttc tagccactcg tcccttctcg tttgccagac ggaccagccg ttgtaaccaa
     5821 tggcagcttc gtcccaattt gggccagccg gaatattgcc aagctgccaa aatcgcaaca
     5881 acccagcaat aagcacgatg aaaagaagtg gaagcttgcc agctattttc atgattggcg
     5941 tttttttagt atccaatacg gttgccataa cagatacgct agtttttgcc aaaaattacc
     6001 atgcttctga acaaaatagg ccgcatgttg ccaactgata tttttaatag cttgacttga
     6061 aaagacgttt tgatgtgttg attcatgttg atggaaaaca atagctgatt tttcaaataa
     6121 gacctgccaa tttctcaatc ttgctctata ggataaatcg atatcctccc agtaagcagg
     6181 agcaaagtgt tcatcaaaac ctccaagact gagccagcgc tctcggttaa ataaagcgct
     6241 tcctccagat gcccaggctg tttcacccga tgcatctgta ttaagcttgc tatgcataaa
     6301 atatccgcgt tcaaaccaca gcttattttt gcccgcagtt ggcagggtag ctaggtcatc
     6361 tgcctcacta ccttcgaatt ctagacagcc tatggcaaaa atcggttgag cattggtttg
     6421 ataggctgta actaaacact tgatggtatc tggtctcagt gacacatctg aattgagtag
     6481 aaatagtaac ggcttagtag caaatcgaac agctcgattc accgatgcag caaatcttaa
     6541 atttttctca ttaacgagta aactaatggt aatcttttta tttttcaagt ttgtaaaacc
     6601 ctgatacagt tcaaatgcgc catgcttatc tgtcagatta ggattttttt gttgagttaa
     6661 cgaaaaacca tacttcagcc agttgccact ttcatcttgg ctggcatcat caataataat
     6721 cagttcgtct ccatcacgta gggaacgcag aatagatggc agatgcgttt ctaatagttt
     6781 tcttccgtta aatgttggga taatggtgga taccgagaaa gaattagaca ttaaacattt
     6841 tcctaacagt actctcgagc tcttgatgag aaaaatgctt ggctcgatgt cgaacaattt
     6901 tgcgtagctg agtttctttg gctgggtctc gcaacaattg tattgtattt gaaactaact
     6961 ctggaatggt agtccaacaa tatgatacta attcctgagg aataatttca atttgacctc
     7021 cacttgcaat gacgagcgga attgctcctg cggccatcgc ttccacggta gctataccaa
     7081 agtgctccac cttttcaggg ctctggtctt ggtcaacctc atatccagta gcatgccaaa
     7141 atatacgagc ctgtgaatag agctcattga gttcggtgtg atcaagctta gtttcaaagc
     7201 gaattggcaa tcctttggcc atagcaacaa ctcgatcgta gaaagcttga ttttcaatag
     7261 atcccacaaa aacaagctcc caacctgcta aaagtcttgg ttgttctttc agtaactgtt
     7321 tgaacccccg aaccaagaca tcctggcgtt tgctatgtag ctgcggaaaa aatcggccaa
     7381 ccgataagat aattggattt ttattcgctt tacttgctag caaggattca tcaatctctg
     7441 gataaagtac ctgatcaaca ctgatgcccc aattgctttc tacgattgct tttgtaaatt
     7501 gagaattagt atttactgga tgccactgct tgattttgat tgattgcaaa aagttgggat
     7561 agttattttt tagtggtgtt tgaatgtgca ataaattatt tctggctaaa ctaaaaaaca
     7621 aacttccgtc tgtaacgtag tatatgtagt caaagttttg agtccatagt agctttttat
     7681 accatggcgc ttgagtaaat agtggtgtgc gaataaactt aattctctgc aacgatttac
     7741 ccaaaaaaac ctgatatcta ttccgataat tttcaacaga agtacgaaaa gaatcatcag
     7801 agatatctga ttcggtaggt aatgcaaaat tgagctcgat tttcttaggg agagaaagcg
     7861 ctacatcaaa gagatgcttt tcaccaccac ctgtgtgaat ttgtttaaga tagggagaga
     7921 caaaaactgc tttcacaatg tttatttatg ctgttcccac aatatttcta cttctctttc
     7981 tttgacagca tacaattctt taatgggggg agtttgttgg agctcccaaa tttgtaagta
     8041 cactaacatt cgattaattg cttgaatgag actttccatc aagcccggtt cgccatcgtg
     8101 gtagccacga tattttatag ctctgcgcca aaattctccc aaaccttttt ttataatgat
     8161 tgcaaaggaa attttagtgg tactagcctt tgccaagagc tgggcttcaa ctggcgtcca
     8221 agccgcagtc ttatacaatc cacccacaat ggttcgatga gaaaaatgaa gtaaatttgt
     8281 ttttaaggtt ccaacggttc ctgaaattat gggtgattcg tgaatatccc ctttccatcc
     8341 tttcaatttt ctcttgtcaa tcagtcgagt tactgtatct gtttcccatc ctccgtattg
     8401 aaatactttg ccaaagaata tgttcgttcg attcatttga aatgctgaat tgttgctttc
     8461 ggaaactact ctctgcattt catcggcaag ttcgggaata attctctcat cagcatcgat
     8521 atataagatc caatcatgag cggcgaactt tgcgccctcc tctcgtctgc gagcaaacga
     8581 ttgatgtttg aatgaaataa ctttacatcc agcattctca gcaatttcgc ttgtgttatc
     8641 tgacgagccg tcatctatga caattatttc tttacaccac gcaatcgttc ttaaacagcc
     8701 gggaagcatc tctgcttcgt ttctggccaa aataattgct gtgactgctg cttttttcat
     8761 actagtgatt gttttccaaa agatccctta taaaagtcta gcactgcttt cctgcgtaag
     8821 cgactacctg atgttagcgc ctgaaactgt aagcgaagca caacggggat taccgctgga
     8881 gtgccgtgag cgaacgctaa caaatatcga tttctttctt gataatacac atgaagcgat
     8941 gagccagaac cgccagaaga acctgcgttg tcgtgccaca ctatagcatt agggcaaaaa
     9001 gcaattgtat atccagcttt cttagcccga aggcttaggt ctacatcttc aaaatacata
     9061 aaatatcgtt tatccaaaaa accaaccttt tctagtacct cacgtcgaat gagcatggca
     9121 caaccagtgg cgaaatcggt agcagcagca ttgttaaatt gtccacggtc aacttcatca
     9181 acaccacgat gaaatgccac gatatgttgc caatcaatgc tgcctccggc ataccaaaaa
     9241 acctctccgc gctcactggg taaataactc ttagaatgat attctttccc tgcagaaaag
     9301 tatattttgg ggcaaataat gccaacctca ggttgatctt ctaatgtttg tagcagatta
     9361 cgtaatgcct gcttgtggat cgtcgtatca ttgtttaaca aaaaaataaa ttgcgagtta
     9421 tttctctcaa ctgcatagtg aatgccaaga ttattgcccc cagtaaaacc caagttggca
     9481 tccgaacgta tcaccttaaa cttttttgat tttgttaatg atttaggtag aacatacttt
     9541 ttaatcgatc cattatcaac aataatatat tgacaagcat atcctgggtg aataagggat
     9601 gataaagaag aaaggcagga attagttgtc ttagtattgt tatagtgaac taaaattaca
     9661 gaaacatgtt tcacaaagct attatagcct aaaatcttag tttctttctc agtagccaaa
     9721 acgctttctt tagcccttta gtctggagac tcatttttga tgcttgccag tatttccaat
     9781 cggagatcac ttgattgatt gtggagtccg taacggtatt tgccgaatat ggaaattgtt
     9841 gtttttccca aattcttaag gcagttgtga cttgatacag actttgtaag agagctaatg
     9901 aagtgccatg taatccgtct agataacctt catcctgata gtaacgccgg aagaaatcat
     9961 taaaaaatgc taaaaacaat tggtccgagt tgatttttgt aagagatttt gatgatttat
    10021 ctcccacttc cgcttgtgta tatcgcatca ttctttgaaa atattcatcc agagatgaat
    10081 aattagtgtg ttcaattgcc aattctgaac tggcatcaat cacccccacc gcaccattaa
    10141 cgctcggttg ctcatgaatg actccagacc atgaaacagc gcctctttta aaaaatctga
    10201 tttggtagtc tggccaccag ccactatgct gaatccaagt gccaaacaca agattttttc
    10261 gggcaatttt atacgcagag atttgagcat gggtgtcatc cacgagtgac agcaactttt
    10321 gttgtaattg atctggaatt gtttcatccg gatcaactag taaaatccag ggtttggtgc
    10381 cctctttgac agatgtattt cttactaatt caacatattc tgctggtggt attgagataa
    10441 cttttgtgtg aaatttgcgc gcgatacctc gtgtttcatc tgtgctttcc atatctaaga
    10501 cgataatttc atctacaaat gagagtgatt tcaggcaatc tgataaagtt ttcgcggcat
    10561 tatgtgtggt aacaatagct gttatttgag acatacttat gctctctctc ttagtaagta
    10621 tagcgtatta cgaattaaac gatccttggc aaggacaaat actgtcaata cataggataa
    10681 aagtgctacg aacattcctg agaccaggaa tcccacactc tgttgccaat acgttctacc
    10741 aatgagagca acggctgcca tgacggtggc cgcagccagc tgcggccata tttgatccca
    10801 caacttaaac gagagttctt tctgtaccat ccaaacagcg gcaattgaag aaaagctaat
    10861 gaggagtgcg gcgattgcga ccccattgta cccaaaccac caaactaacg gtggagtaag
    10921 aacccaggtt aaaagtgtcc acatgatcat taattttaac gtttggttaa ttttgcccat
    10981 agcattaagc aaattggtca acggtgttga aatcgcactc caaccaatgc tcagactaaa
    11041 taaagcaaaa ctaaacgctg cgggcatcca cttgccatat gtcgatgaaa atactgatag
    11101 aaaaggagta ataaaactag ccatgccaac aataattggg aaaatcgcca gggtaatcca
    11161 gaaaagagaa atctctaagc cttttgccaa agcgtcttta cggtgctgta atctagaaaa
    11221 cgtcggaaaa gtaatcgcca tcacgttttg gacagtaagt gtgtaggggt acattgacca
    11281 attttttgac cattgaatat aaccaaattc tcttaatggc atcatcacgc caaccacaat
    11341 gtaaaacagt tgatctttaa ttcttgccag aaaatcgttg agttgaaact taacaccata
    11401 tgaaagcagt gttttgattg ccggcttagt aattgctagt ccaaattgcc attgctgaat
    11461 tacgttcatc aaaagaatgc cgattattgt tctggcgcca attgcaaatg cataacttat
    11521 tactccccat tgttgaaaag ccataaagat cagaacccca tgaaaaacaa tttgttcagc
    11581 aatctgtggt atgactaatt ttgagaattc caattttcgt tcaagaatta cggagggaat
    11641 ggtttttaag gttgttaatg gaaaggaaat ggctagcgtc agtaaaatcc aatcacctgc
    11701 ccaaccagtt tttctggaaa caatacccga cgctaccaat agcacagcaa taacaacaat
    11761 gaaccatcca agtagttgtt gaatggtaaa cgccgtgcga tattcttcta cagttggatc
    11821 ggtttttttc tgtatgagcg cagcggctaa accaacgtca gaaaaaaacg tcaataaacc
    11881 aatgatcatt agaataaaac cataaatacc aaagtcttct ggagcaaata aactactcaa
    11941 aatggctagc gaaagaaacc caataccttg caaaataata gttcgaagta agtatgacat
    12001 ggcaccagaa atagaccgcc gtttgattat ctgaagttcg tcattcgtag tggttttctg
    12061 catagcttag ttttgttttt ttgtttgtgt actaaagagt ttaacgaaaa gaaggatcac
    12121 tgcaatcgta ctgatgagat aactccacat ccgtaccttc gtcgctccaa aatttaaatt
    12181 aagcaggtgt tctccttctg gtagattcac ttcaatcagg ccgttttcgc tggttaaaat
    12241 ttgtatttct gcaccatcaa gatatgcttt ccaaccaggg aaatttgcaa ttgaaatggt
    12301 tataggaact ggctctttgg ctttaactaa gagtatcttt tgagacgtag catcttttaa
    12361 gattgtaact gtaagtaatt cgctctcttt gaccaactgt tggaacggtt caatatctgt
    12421 attgaaagtt ttgggtatgt aatctggcag aataccactc atttctgttt ggattctcgt
    12481 ggcgtctgtg tagtatggac cattgtttga atcataatag gcagcgggta caaaatatct
    12541 gaatgaggtg attagagtaa ttgtaacgat ggtgagcact gctgctcttt ttcgtttgaa
    12601 attatttgaa aaaaacatgg caattattac tgtggctata aaaacaattg cgcttaataa
    12661 tcgccagggg aattgaagat acgcaatcat tggcagattt tcccacaacc aaagtgactt
    12721 ttgagtagtg agtaagatta gaccacccat agtcataaac agtaaaaaga tactcaaaga
    12781 agtttgttta ctcactcgct taaaattctt cagagcgtgg aacactgcaa gccctaaccc
    12841 aaaaagtaca ccatatccca aaaagaaatt tatgccgtcg ttgggacccc attccgaacc
    12901 accatatttc caattatcta gcaaaaattg cctaagatac aaaaagtgat tgtggtaatc
    12961 aaaatagcca gttaagattt gctcgtccaa ccgagtgaat gatttctcaa acaaagccgg
    13021 cagagcataa aaagcactca ggccaaagcc caataaggaa gcaagtagac cttttactag
    13081 tagtgatagc atttcttgaa tacttcgctt tatatttttt cgtttccaga gataaagcaa
    13141 caagccagat agaaatccga gtggtacaaa gtatagggcg gataaattgt gactgagtat
    13201 tatgatggcc gtgctgagtg atatgtatag ccagcccaag cgatgttttt ttatcatcaa
    13261 ataccaaccc aacactaacc agggtagcaa tgccattccc cataattcat tgattgctcc
    13321 tctcaaaaac aaattaactg cccgatatgg tgcaagtgta tatagcgcgg caaaaataac
    13381 ccccccagcg tttccagata tctcctttcc caatcgatac gatcccagaa tcgtaacaat
    13441 atttacagaa ataaatagta agagtaatga caaagatata gagtcagaaa ctatataaag
    13501 gaaggcgcca atataatatg ggaaaggtcc ataaaacaaa aacaggggca taccatatcc
    13561 atagccaaag tttttcgacc actgaactgg aaagtcccca tcttgaaggg cgcgagacat
    13621 ttcggcaatc ctggcagcat gagtaaaatc gtgcaaaaaa aagagtttac ccaagaggaa
    13681 acttcttgaa gatataaaca atataacaag caacatcaag aacacaagca ctcgtttgag
    13741 catcttttca ttgtaccgca ttactaatgg taagatagct actaatgaat cgaaacttaa
    13801 ttaagctaat gctgaaagcg cagttggcca ccgccggttt tttattggtt ttatttgtat
    13861 ttaccggatt aattctcggt ctaattggat ggtatcagtt ttcactgttt caaaaatcaa
    13921 gcgggctaac tactacagaa atacttcgca tggcaaaaaa tgcttggcag aaccctttca
    13981 atcactcgaa tagtagaatc tcaatactag tattaggctt agacgaagta gaagcccgct
    14041 cgcaaaccgt tctaacagat acaataattc tactaacgat gaatctggaa actggtgaaa
    14101 ttaactccct ttcgctaccc agagatcttt ggattgatga ataccagaca aaaattaact
    14161 cactgtatta ttatggtcaa aaagccaatc ctgagcagcc aaccaacctt gtgacttcag
    14221 caattgagtc tctttctggt atctctatcg atagaattat tccggtcaat ttagaacaat
    14281 tagcagaatt aatagacaca gtcggtggat tggaaataac tgtagctcag gcatttactg
    14341 atgatcattt tcccattcca ggtcttaacc caagtcaaat caccgatcct acattattgc
    14401 aacaaacagt tagctttgaa gctggaactc agacaatgaa tggaaatcgt gtattagaat
    14461 atatacgctc tcgccaagct attggtgaaa atggtagtga tgattcccgt acgagaagac
    14521 agcaacaagt aatcgttgcg ttattagata aactgcagtc aaaatctgtt ctgactaacc
    14581 ccgaattatt gggtagactg gtgaattggt atagaaggaa ttatgaatct tattggccac
    14641 tcagcgagat cttgcagatt ggggttcaat ttgtaaaaaa tgatttacgc ccaaaaatca
    14701 ctacgcacac attgccaatt gcagaaaatt acggggagta tggagtactc atacatccac
    14761 ccatttggag atatcaaaat caatgggtgt atatcgctac tgacagcgct atcctaaagg
    14821 aatacataca atcttcgttc gatcttaact caccccctac tccgcaataa agacatgaaa
    14881 ttaccaaata ttgctcttcc atcccaaaaa accattactc gcttattgtt tttgtccact
    14941 attatttttg cacttttgac aggactctat caagaactat accgaattga acaaaaaaag
    15001 tatgcaaatt tagagaatat gtatgttaga gtgagaagtc agttgggtag aaccgaaaca
    15061 cagcgcctaa ttgatgagtc gtataataaa taatcaaaac caaaatatga acaaaacact
    15121 cgataaacac aatacgctag tggttacagg aggggctggt tttattggta ctaatttttg
    15181 ctattattgg aacaaacaac atccggatga tcgtattatt atccttgacg cactcactta
    15241 tgctggcaat cggtcttctc tagcagaatt agaaaaacag agtactgtgc gcttcgttca
    15301 cggtactatt aacgacgctg cattggttga cgacatactg agcgaggcaa caatggtgat
    15361 tcattttgct gctgaatctc atgtagacag atccatcact aaccccaatc tatttctcca
    15421 aaccaatgtg atgggtactc ataccttatt acaagccgcc ctcaaacact ccgttgccag
    15481 attccatcat atttccaccg atgaagtttt tggttcgctc cccttagaca gcagagaagg
    15541 tttctctgaa actactgcct actcaccaag aagtccctat gcggccagta aagcggcatc
    15601 tgatcacttg gttcgggctt atgccgagac gtatggatta ccggttacca ttactaattg
    15661 ctcaaataac tacggcgaat atcagtttcc tgaaaaattg attcccctag caattacaaa
    15721 tttgctcgag ggtggtacgg tccccattta tggtcctggc aatcaaatta gggattggtt
    15781 atatgttcaa gatcattgtc gtgctattga actcgtatta ctgaatggcg aagttggtca
    15841 aacctattta gtaggaggac ttactgagga tattacaaat ttagagctga tcaaattagt
    15901 attaaaaatt atgaatcgat cagaaagttc tattcagttt gtagctgatc gaccaggaca
    15961 cgatcaaaaa tatcgagtta attggaatac tattcaatct caattgggct ggcagccatc
    16021 ggttgatcta gaacaaggtt tgcaacgaac aatcgattgg tataccaata atcaagcatg
    16081 gtggaaacca ctgaaagaag ccaacgctgc ctatttttct gcacagtata atacagcgga
    16141 aagttcataa ctatgccaag tgataatttt gttccaactg aatctaatca tgttgttgac
    16201 ggaatctatc aaactgcaat tgaaggtttg ttattttcag catcacaaaa acacatcgat
    16261 caccgaggtc actacgccga acttgttcgt ttgccagatc taaatatggc actcaaacaa
    16321 cctttcgtcc cagttcaaat taaccactcg cactcacatt taaatgttat ccgaggtttt
    16381 catgccgaaa attggaataa attagtgaca attgttcacg gatattgcta ttgtgttttg
    16441 gttgatgtta gaccgaattc agcgaccttt ggccattctg taagtttcaa tctgggcaac
    16501 catgacgacg cgctctatgg ctcactctat attccggcgg gtgtagccaa ctctgcgcta
    16561 gcactctctg aacccgttga ttatctctat tttgtagatg cgctctatgc caaccgcgat
    16621 cgcactggag atgtatcaat ctcattattt gataaagaca ttaatgctcg ctggcctatt
    16681 gcccaaaaga cgatgatagt atcagaaaga gatcgtcaat cagtaaccct acgagaaaaa
    16741 tttcctaaca agtttccctc ttagattatt tagatataac tatgaataac acaaaaaaac
    16801 catcaattat tggtacgggt ctaaatgggt tagtgggttc aaaaatcgtc aaagatttat
    16861 ctgccgttct tcaatttgag aatattgata tttcagataa aactaatccc gttgatatca
    16921 caaatattca acaagtagag agttttattg gcaactctgc cgcttcggta gttttgcatt
    16981 gcgcggcatt cactaatgtt accgcagcct gggaacagac tggaaacaaa tcgggtctcg
    17041 cctatgcaat caacgtagtg ggcacaagaa atattgttaa tgcttgtaag aaatacagca
    17101 agttcttaat ccatctgtcg acagcatatg tatttgatgg aacaaaggat gaattgtata
    17161 ccgagttcga cacacctcac ccaattgaat ggtatggtca aacaaaactc tgggcagagg
    17221 aagaagttac taactccatt gatcaagctg ctattcttag gatcgatcaa ccgttcagat
    17281 ctgactctta cccaagggct gacatcctcc atcgaattct caatcaacta actgatgact
    17341 ctttgcctcc tcaatttaca gatcattcat ttggcccaac ctatattgac gatttagcaa
    17401 aaacaattct ttggtttact caaacaaaaa ctgcgggtat ttttcacgcc acaaatggcg
    17461 agcgatggaa cgattatgac tttgctcgca aagtggctga ggcggcacag ttatccacta
    17521 tagtgaagcc tgggtcgctc gccaagtatc tcacaacatc gaaacgtcca tatcaaaaaa
    17581 atacagccct agatacaact aaattactac aagttatccc tcataaaccg acgcctattt
    17641 ctgtagctat tagggaagca tgtacttatt ttgaatccaa tgagtccaaa taatcggctt
    17701 tccacgcctt aatggctgct gcgttacccg ataacagcac ttcagggacc ttccaaccac
    17761 gatagtctgc tggtcgtgtg taacttggag ggctagagat tcctggctgg gcgtgacttt
    17821 ctgcgacaat gctggcctca ttacccaaaa cattgggtaa taatcggcta atcgcatcaa
    17881 tcataactag cgccgctggc tctcctcctg tcatcacaaa atcaccaagc gaaatttctt
    17941 cgtctactaa atagtccgcc actctttgat caacatctcc gtagtgacca caaataagtg
    18001 atacagattg atatgtagct aatctactgg catcagtttg aacaaagcgg ttgccacgtg
    18061 cagaaaggag gatggttctg ttcttttttg gctgggatat tggtatactt gccaacgctt
    18121 tctcgatagg ttcaattttc atgaccatcc cagcacctcc accaaaaggt ctgtcgtccg
    18181 ttgttttatg cttatcattt gtaaattgac gcaaatcaat caaatttaca tcaataattt
    18241 tgttggaaat tgccctatga atgatcgatg agtccaaagg actctcaaaa tattgtggaa
    18301 aagttgttaa aatagtaaat atcattgtct gctagtcatc atgagtacta acaggtatga
    18361 ttatactgta cgaagtttta aatatttaca tgccagcaac aagtaattcc ggccttacct
    18421 tgacccaagc gaaaaccttt agtaaaagct ttgtgctcta tggatccgtc ggactagtag
    18481 tgtttttagt attttggttt gcatctggag catttattcg gtactggatt gccacacatc
    18541 ctgagccacc tcctcccccc accgtaggtt ttggtatact tccagcacct caatttccca
    18601 tcacaccaga tgaaagtaag ccaaaacagt atatattaga aaccccaact ggcagactac
    18661 cggatttttc tgatcgcgcc ctcgtatttt ttatgccaaa aaattctccc ggacttcttg
    18721 atgcagaaag atcgaaaaat tttgcttcag aatatgagtt tctctctgca ccagaaatat
    18781 tagactctag aacgtatctt tggcaaaaaa cacaaccgct ttttactaca cttgagtatg
    18841 acattcagga tcaggtattt agctatgtca gtgatttctc aaaccgacct gaattattat
    18901 tagactctga attaccaacc aaatttgatg ctattaacga agttaaaagc tttatcaaaa
    18961 aagcggttga cctgccgtca gatatctcaa cagcatctgg cacttttgct tttgtaaaaa
    19021 gtgtgggatc agacttactt cctgctgttt cggttactga tgccaacttc gtaacggttg
    19081 atttaaaccg atctcccata gaaagtaggt atcaaatcta cactgatcgt gatggcagag
    19141 gcgtcattca cgcgctcgtg ggaaatatta aaaacacaga aaagattctt cagctggact
    19201 attattacta tccaattgat tattcagtca ttcactcata cccattgagg ccggttaagc
    19261 aggcttggga tttgctacag ggtggtgaag gttatatttc ccacgcggta tcttccgatc
    19321 aggctactat tagggaagtc acacttggat attatgactc tacaactccc caaatgtact
    19381 tacaaccgat ttatgtattt tcgggtgatg atgggtttct ggggtttgta ccggcaattg
    19441 atcctagttt cttgaatact acaaaaacta acctaccatg aaagttgaat caataagaac
    19501 tcgagttgct ccaagtccaa ctggtttacc ccacattggg acagtttttc aggctttact
    19561 agattatatt attgccaaac aaaataaagg aacatttgtt cttcgtatag aagatacaga
    19621 tcaaaagcgg cttgacccac aagctgagca agctgtatat gaggcgctgg agtgggtcaa
    19681 tattcagcca gacgaaagtc ccaaagctgg gggcgctttt ggcccttaca ggcaatcaga
    19741 aagattgctg ctgtaccaca aatatgctca acaattggtc gaccaaggcg cagcttatta
    19801 ctgtttttgt tctgcagagc gactcgagtc tgttcgtaat caaatgcaga aagatggtaa
    19861 gccccccatg tatgacaaat tatgtcggtc aattagtcct agcgaagcaa aagagcgagc
    19921 tgctgcggag ccacatgtga tcagattaaa agtgccaaca caaactcaga tatcagttct
    19981 tgatgcggta cgaggaacca ttgtttttaa tagcgacgat atcgatgatc aagtgctact
    20041 caaatcagac ggttttccga cataccatct cgctgtagtg gttgatgatc atctcatgga
    20101 aatcagtcac gttgtgcgcg gtgaagagtg gcttagttcg gcacccaagc atattttgct
    20161 ttacgattat ttcaattggc aaaaaccagt gtttttccat actcctactc ttagaaatcc
    20221 agataagtca aaactttcca aacgccatgg acatactact gtcgagtggt atcgctcgga
    20281 aggaatagtg cccgaggcgt tgattaattt tcttttgtcg atagtatgga cacatccaga
    20341 tcaatcagat attttttcat tacaggaagc tatagagctt tttaaatttc aatctgtgca
    20401 tattactgga ccgattgttg atttggcaaa actgcgttgg attaatggac aatatattcg
    20461 tgcctattcg aaaaatgatt ttgtaaatct ggcacaacca tttcttcccg ccgactttcc
    20521 caaggaaaaa gcagcttcaa ttttaccact cattcatgaa agacttgagc aattaaatca
    20581 gattgaatca ttaactgact tcttttatcg accgatcgtt cttgatgaaa aacccctcct
    20641 ccaaagatcc actgctgaag tagttatcaa tcaactgaca ggcacaattg accgtcttga
    20701 agctttacca gaatggaatt tacaccaaat tgaagatgca atccgctcat tgcaagaaaa
    20761 aaacgactgg caaagaaaac aatattttat gttgcttcgc tatagtacaa ccggaaaatc
    20821 tgctacacct cctttgtttg aaacaattga agtgctggga aaaacactca ctatagatcg
    20881 cctcatttct gcgagaaccc ttattacctc ataactatgt ttcaacaaca agatctcact
    20941 caacaagcag atttggcata tgaacatttg ttacaggtgt ttcctttggc gaaaaaattt
    21001 catccatcct tacatcaacg aatttctgtt gatcaatctc tcaaacaacc tctgcaggaa
    21061 tttaataaat ctgaaaagtc aattattttc gataaacttc gccaaacgca caaacaacca
    21121 ctttctttgg taccctcaga aactttgttg tatttagaac agcaattggg tgatatcttt
    21181 ggtttggcgc ttacctcgga aactaactta ggctcattgc cctatttggt gggtcagatt
    21241 gaggctcaac cccatttgaa atcatcccca acggatacga ttaataatca cagttctatt
    21301 ttggaagctg gtttacgacc cgagcgcagt tattatggtt ggcaatctga accctacgga
    21361 atttccttgc cgctccatga attagctggt tggcaaactc tctctgataa ggaaagaaaa
    21421 gcgtatttaa gccaactaac tttagtaatc aacccctggc tagaaattgc gattgttgcc
    21481 aaaattgtag atataggccc ataccatact caaagatatc aatttgctgg ttctccacag
    21541 ctagttcgag agggaaaatt ttggaatcct ggttataatg gtttggctgc tattttcttt
    21601 cttgaatcta attccactca tttgaaaact ggaccgctat cttatgaacg ctaaagtaat
    21661 acaaagcttg actgcaatag taccaacatt acgatggtca gatgatgtga caatccagca
    21721 cctacaatta tcaaacagcc tgactgttat agctgagcaa ttaagtccca accaacaggc
    21781 tgaactagcc gaaatgattc tagacgcgga tactcctccc aataaaatag cgaaatggct
    21841 cactgatcaa attcctcaaa tcacattact tctgagagaa ttagaatcta aagttactct
    21901 cataactaga aatatgcaac catgattaca atcgctttaa atattggctc aagatttcct
    21961 gtgaatcgga agagaatacg ttcatctgct atgaaaattc taaatgaata taaaatagag
    22021 aatgcgcaag tcgatatttc ggtagttgga gtacgaaaaa taactgaact gaatgaaaat
    22081 cgattgggtc acgatggctc aaccgacgtt ttgtcatttc ctctgcacga aaagaaagca
    22141 ctagatgatt tcccgcttcc caatacaatt ccgccacatc taggagatat cgttattagt
    22201 ttcccagctg cacttgaaca agcgaagcga tttggtagac tggtggacga ccaaatctgt
    22261 ttttttgtag aacatggcat gttgcaccta cttggatacc atcatgaaga tgaaaactaa
    22321 tcaatcactc gcttggacta ctagctaccg ccccatttcc ttatcgcaac tccatcaggt
    22381 ttctgtcaga aatcaactga ttcaatacca aaatgaggga tcatttccac aagtactgct
    22441 ctttgcagga cccaagggaa caggtaaaac tacctctgct cgcattattg ccgctcttct
    22501 caatgatcct aaaaatacca aaattgtaac taataattac atccaacatc aaagcgaaaa
    22561 aaaagcactg agtgacccag acgtttctaa tgagttaatt gcaaagattc tcactggcac
    22621 gtcatttact gttcatgaat tagacgctgc gagcaaccga ggaattaatg aaatgagagc
    22681 cattaaagat caggctcaac tgtctccaat tgaaggcata atttcggttt tcatcctcga
    22741 tgaagttcac atgctaacag cccccgcctt taacgcgcta ttaaaacttc tcgaagaacc
    22801 accctcacac accacgttca tacttgccac cacagaactt cagaaaatac cagaaactgt
    22861 ggtgtcaaga tgtcaggtag tgcattttca ccaggcatcg caagctgaaa tacagtctgc
    22921 gcttgagttc gtaacccaat cggaatcgtt acaaattaac gcagatgtgc tggctagagt
    22981 cgctgattta gccaatggaa gctttagaga tgccgtaaag tacttacaaa agattgctac
    23041 atataagaat cctactttag atctggtgga agctcttatt gctccctcct tttctgcaag
    23101 aattgattca attattactg cactactaga taaagattca gcaatgattt gcaagttgtt
    23161 tgttgatctt cgttcggagg tctatgatga gaagctattt attactcagt tattagaaac
    23221 tattcatcaa gatttgttac tccactatct accagaatta gattctcagc cagtccgcac
    23281 agttgcaata aacctgtttt tactaaaaga atttactcag tccatttcgt acccccccaa
    23341 ccccattcct tttttgagtc tagaaatcac ttgcttaaat ataattgagc gatctcaaca
    23401 gaagacaaaa aaagggagtg gtgacaatgg aacaacggta actaaaaaaa taacacctca
    23461 aagaattgag gtcaacaact caagcaattc caaaggggat gcacagaagt tgcttgaaaa
    23521 ctggcaacgt tttttaactg aagtagccaa ccacaacact acattagctg cattatttcg
    23581 atctgcccag ccgaagcgag caaacgatca tagtttagaa atagaagtgt attatcaatt
    23641 tcacaaagaa cagctcataa atctgaaaca tcacgctgcg attcaaccag tgctcaatac
    23701 tttggttggc ggcgacgtgc attttgatat atcagttgta actgcgcccg tagcggcaga
    23761 gcttcttgat gaagttccca caagtcttcc agaaagggtc gccgccgcac ttttacaatc
    23821 agatccccac tctgcaacag atgtagtaca atccaactag tgtaatttag aaaggctact
    23881 atgcaaaatc cattaaaagc gctcggtgat ttaaactcat tgcgtcaaca agccaaacaa
    23941 atgcaagatg aacttggtaa agaagaaatt gtcatcgaaa aaggtgatat ccgggttgtg
    24001 attaccggtg atcaaaaaat taagcaattt agtgttcagg gaatttcaag tgaagatgca
    24061 attgctgttc tcaatgaagc aattaaaaaa tctcaagaat tagcggctaa aaaattacaa
    24121 agcatgaccg gcggtctggg tggtttattg ggtaaaatgg gtcaataaat gtcgccacgc
    24181 ttgctcgttg cctcaaccaa tcaaggcaag ttgacagaaa ttaaaggtat tctcagcgat
    24241 ctaggcctag atattgttaa tctttctgag tttcctaccc tcgtagtttc gagtgttgaa
    24301 gaaactggta ttacatttgc tgaaaatgcc gcgatcaagg ccaaacacta cgcatcaata
    24361 acgcaattgc cgacgcttgc cgaagactct ggattaatta tcactgctct caatggtgaa
    24421 ccaggggttc aaagcgctcg ctggtttcca ggttctgacg aagctcgcag tcaagccatt
    24481 ctatcaagaa tgaaaactat ttctaatcgt tcagcttttt ttcattgttc agtttgcctg
    24541 tatcacccac tcactcgagt gacacaaacg tttgatggtg aggtggaagg cacaattgct
    24601 caagaatgtc gtggggagga tggctttggc tatgatccta tttttatacc aaacgggtac
    24661 tctgaaactt ttgctgagct tggc
//
LOCUS       DAOQZP010000007        24113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_2446_length_24113_cov_5.453986, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000007 DAOQZP010000000
VERSION     DAOQZP010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 24113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24113
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2446_length_24113_cov_5.453986"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..686)
                     /locus_tag="PKH60_00775"
     CDS             complement(114..686)
                     /locus_tag="PKH60_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNQ16616.1"
                     /translation="MLGLIKLTISKKNVFIFIYLACLIVVTTATFFLYAEPLGQVLGQ
                     QDAIRQEAKTQHVESEFFVVRVIDGDTIELESGQKVRYIGIDTPEIHHPTKSVECFGE
                     QAAEKNRMLVEGKLVTLEKDVSETDRYDRLLRYVYVDGVMVNELLVREGYSRASSYPP
                     DIKYQELFSEAESQAQQAGEGLWDKTICEY"
     gene            complement(885..1094)
                     /locus_tag="PKH60_00780"
     CDS             complement(885..1094)
                     /locus_tag="PKH60_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16617.1"
                     /translation="MAWALFDLDPMEVADAQLHTYKSSGKSFGREKKKKLIDQKRQAR
                     KRSRRRKNKMAMRSRRSGRAKKVRY"
     gene            complement(1465..2661)
                     /locus_tag="PKH60_00785"
     CDS             complement(1465..2661)
                     /locus_tag="PKH60_00785"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNQ16618.1"
                     /translation="MSQMYGGRPRSYGSFGGSRQRGPIQPRRNMGAYIAPSRYVQAAQ
                     KPEQEEPYQALNTFSDFGLDSQLLANIQRHGYTLPTPIQDQAIPELMQGKDVIGIANT
                     GTGKTAAFLLPLLHKVIQDPNQGVLILAPTRELALQITEEFRAFANRLPIGVCVCIGG
                     TNLDTQIRQLKKNPHFVIGTPGRIKDLIDRKAFKTSLFTNIVLDEVDRMLDIGFRQAI
                     QFLIAQLPPVRHAAFFSATMNADTDAIARKLLTNPVTIQVSKQEFNTHIEQDVVKLLP
                     GQNKIDILHGMLLQTEFKKVLVFGRTKHGINKLEMTLSKLGVRVTSIHGNKNQNARQR
                     SLEAFKRGHVQALLATDVAARGIDIDDVTHVINYDEPQSYDDYVHRIGRTGRAGKMGK
                     ALTFVM"
     gene            complement(2764..3441)
                     /locus_tag="PKH60_00790"
     CDS             complement(2764..3441)
                     /locus_tag="PKH60_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16619.1"
                     /translation="MTEPSYQAGYNNDRDFVSGSEPALAASLENLLQSLKLRRGQANA
                     GDRSSSWGDAFFARERGRDRYQQFGEKNKPDPLFEQLQTINQATDNAKDGLPENSKEA
                     QQLCDEAIKSFAQAMRQHSQEITPEMLMKSENEMRDVLDPASIFVLRAICGLEIMTYK
                     VRRKIRDSWCEVDLPPELFDILLLDDDEFKREILAYRFLYPYNQLKIQAFARCLKQDA
                     SLFAPVH"
     gene            complement(3532..4224)
                     /locus_tag="PKH60_00795"
     CDS             complement(3532..4224)
                     /locus_tag="PKH60_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16620.1"
                     /translation="MKFIEGISGYQEKREQTQQVADFLELVTTSPIPVEIATRSLTGE
                     KLFVWVNTIIPPRDKKYEIITDSVEELTTIVAYVQSNGTLFPVDEYGLIAHCDLAFCR
                     KQTGEQYVFTDLKCPPSQYVPPENLGWKISAESVYWFVNSAMTLERRRPLLIGEPDNY
                     AVGIGSLLNAIGVEMMLRRNIFLVQSYYLSENGQKIWEQLGLSKSNHFFRYLDPVVAR
                     AAVNNTILQHSY"
     gene            complement(4388..4537)
                     /locus_tag="PKH60_00800"
     CDS             complement(4388..4537)
                     /locus_tag="PKH60_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16621.1"
                     /translation="MSKKSVAKTTNPLTTVTTLSKVLAAVLFIVLPFIGAYLGYLYFP
                     ILNNF"
     gene            4573..5253
                     /locus_tag="PKH60_00805"
     CDS             4573..5253
                     /locus_tag="PKH60_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16622.1"
                     /translation="MPFFTQLKQLFFLLLCLIIAGLTVVAILLWLKVDRTEQLLTNSA
                     QETVSPENSKNLATSSGRLSAIDQDQLEASVSALAARLEEVEKNQKTIQSQIATSASA
                     QQKNSTPLFTKETLYLGWASTNSREWSETGLEIGINSAHYPAGVTVKLEASLSIIGGE
                     AWVRLKNKSTGAVISISEISNNTNTATWKLSPSFALHTGGYTYVLEARSTSGETANIS
                     AARLIIEK"
     gene            5316..5948
                     /locus_tag="PKH60_00810"
     CDS             5316..5948
                     /locus_tag="PKH60_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16623.1"
                     /translation="MNRNESWTRSIKDASPFRFVYPEFLTHLQSLELQPKHISGSVLV
                     IGQSAACPEKTLLATPESSFQVLRREVTSVFFCDSDLFTAPHIHFDNPVINTIDFDIL
                     PQPRQGEIYFEMSSSYDFLQRAPTNFFDSALMIRVEDTGTQIADYDLIKQLAPHLKPG
                     GFFICSGGRFPTHLKSEFFSPLSPVRVVQLPNYSGGYPFINNMGAILQKM"
     gene            complement(5976..6278)
                     /locus_tag="PKH60_00815"
     CDS             complement(5976..6278)
                     /locus_tag="PKH60_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16624.1"
                     /translation="MKERETGVEGKLAAKVVTLGGLNSDHILSIIEANAPVPLSWEVT
                     EAIHAYAKGNPGVAVVLSKHAACLKTAVDVEELVGWPLSVHTIERTGDIWDKLKNS"
     gene            complement(6405..6800)
                     /locus_tag="PKH60_00820"
     CDS             complement(6405..6800)
                     /locus_tag="PKH60_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16625.1"
                     /translation="MSEKELQPNKRYGNDLFSVTVLSEIPAVVPFLENQDYCKVDADC
                     SIRYNWCEKGGWNDYSAYVEDIGCTAGFNEGQSESMAEQLECDVSYFPGMVASYSSVR
                     CIDNSCVAQNPSFSCPSDTDSWVNDGLVL"
     gene            complement(6828..7277)
                     /locus_tag="PKH60_00825"
     CDS             complement(6828..7277)
                     /locus_tag="PKH60_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16626.1"
                     /translation="MDSQAIPPIEQQLPKETKSSTFRVKPTSIIIIFLVLLSVILMLQ
                     NRQLKLSKIAQNADVIELEQVLDASSPTAVAQTDNLNPVATNTELTSLRAYSIKVGGT
                     KQDESLQLMVYKTLFGDEWTTWSNNRNWSVIYRQYSQSSSEIYKKSF"
     gene            7367..8989
                     /locus_tag="PKH60_00830"
     CDS             7367..8989
                     /locus_tag="PKH60_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNQ16627.1"
                     /translation="MLLEATNLAKSIGAKYLFQDLAFTIVAGEKIALIGRNGYGKTTL
                     FKILSGEDHDFEGTVTTRKGTRIVLTKQEHIQELEQSALEYILNSVPHYFEYQKFLEE
                     YESGQNTNLNRYLEVLEYFTEHKYFNLREAVISSLEDFGISTVMVHQPLKHLSGGQKR
                     YVEMTRMMFSQADILLIDEPTNHMDYKGKERFITWMRSILQAVVVITHDRDVLKNVTK
                     IIELKDQKMHFFKGNYHSYIKQNATQTLTSVKLYEDQLSRLKEAKKKVEWGLQMRAKS
                     KEWKIRYDHWLRDYEKIKAATIKPAFWIDQNSIESLDKKVVDSYDKFKEKNIKISGTG
                     DVKRGGELVSVRQLAVGYDECLFSEINFSLKESGRVFIKGRNGAGKSTLVKTLIALSE
                     KQPPTANLLDGEITFGNDLRIGEYQQEIDGKYLSLPLVEAIRVIHEEKKVAIDERKVY
                     SLLAQYLFDTKLDAHQKIANLSGGQKARFQLIKMFIGEPNLLILDEPTNHLDLPSIEE
                     LETMLENFPGAILYISHDTYFINRLGGDAIQL"
     gene            complement(9299..9643)
                     /locus_tag="PKH60_00835"
     CDS             complement(9299..9643)
                     /locus_tag="PKH60_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16628.1"
                     /translation="MPEVAPSRTDSYPFQYRPRDIQVIAEMIADGSSISVTVSSRAHA
                     EDLVERIKNHLSQLDAAQPIVLSLNSPALEADIARVVDIAMNSQGFPSAFKQQHPEYY
                     VWVNADHILNTV"
     gene            9817..10992
                     /locus_tag="PKH60_00840"
     CDS             9817..10992
                     /locus_tag="PKH60_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16629.1"
                     /translation="MNNQDFPPSNDQLENELLLQPPKNQSKITLVLTTLLIVAVGVAI
                     ALGYQLFTQNQKIGLQPSTPTTPPTSATLSPTATAPESRTYTDPKSRFSFSYPQSWQV
                     NDKSSSNQDPQLTFTNIPEGHTINVSVWRVTGFGYCYIYGEKQTIVVGTKNAETADGV
                     GPSEMCDKPEEYANRGNTYVLIPLEDTTDGLPLNQLHISYDYPLSDKSIAKTYLNQIL
                     SSVKLTTNLTANNQPTTSILEVAKNVQQYDDKGNTIPGVASVDIIIDLGANASVVQTE
                     RKIKGMDVYEQTVKTANSEYSFTSAAGGLCPWEDDGSGCTYTDEKINKFGEDAKVRVW
                     RDNQRGVFALNFQSLEIGNYYVDHFEIFKRSPNESFTASEITTWKNWVMNIKTIPHI"
     gene            complement(11123..12271)
                     /locus_tag="PKH60_00845"
     CDS             complement(11123..12271)
                     /locus_tag="PKH60_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNQ16630.1"
                     /translation="MSTFRKFGWLLVLSMTVVVLLSACSGTEPIVSAGQVAVKLNPAF
                     LPGGNEPRYTSVITAGNRYDVNDKGYELKKILIVPEGKLGWDGTRNLLPGPYPNLAEG
                     DITIFRDMTVITTGWIAVHSTGDGTYQALQPGRHAVPLEEVIRIPTGSLRYRTLASEL
                     LPDENACESFKCETSITRASVSGSQVEATVDVDVLFHFDTTDPQALWKLQDLEVAVQN
                     FVGSTLRGSRAASSPFTIEQLKTQAGREALQEAYLQPLKASTAATPIIVDQVLVRSIT
                     FGDEAWRQAQLQREQELAVEQNQAALLQEQQKNLAAENKLKAERAAFAREQALLDADN
                     RVQVAQKLLTAYSTSDWRILWVLMYGEQVPPFMNADGNLSQQPELLRP"
     gene            12824..13279
                     /locus_tag="PKH60_00850"
     CDS             12824..13279
                     /locus_tag="PKH60_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16631.1"
                     /translation="MGDNELGVHSVDGVLVNRVKRVNFDDLLYGKRELMPESAGLPNW
                     LRRNAPNFEVLDQIFDQFVERANGADIEIDDLKTIHFLISYDVSLLRNFESGIAMARH
                     YGELEGFDDQPLVSLIETAISKRVAFLETLTNQIDTLLSAEKNGEVEIK"
     gene            complement(13347..14339)
                     /locus_tag="PKH60_00855"
     CDS             complement(13347..14339)
                     /locus_tag="PKH60_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16632.1"
                     /translation="MVENAALNPHLRFVGVPESSPWIKGIRDVADQIVEASQEEGMIE
                     IDIDDSDVLREVKQLLTAHFSNLNLEEAMLLPNHVFMAPGTSNKGHGIPFVGATSDDG
                     SILVFEYSPTLETEEYRDWNELLRTSIIAHEMYHATAPTIFAPVWNGVARESSGLNYA
                     KKPGAVEEGLATRFQIEAYTIIARHFRISGNMFQFYRNNLEGFNDRAKDLIYLRGIDE
                     EGGAIPAFLPYKDSYVLVDWLINGGSDLIEILSPQFRAKYEKLFRDFSGIQGMEKLME
                     GARLKKHTLAFAKKIEGVFGAGSFRELVTSDSTQAEALLGRLLDRLAESKRVVT"
     gene            complement(14713..15555)
                     /locus_tag="PKH60_00860"
     CDS             complement(14713..15555)
                     /locus_tag="PKH60_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16633.1"
                     /translation="MEMISEEIFQPPESSPNLDAIEQQVDFAIATKSNIKKRIVTRLA
                     MTVLVGALFLSACSTVETQGNPPPQYASATEFARTPIALSEESSHTYSPDRVIFEPYK
                     ELAPYAINLPAQLKEDLVKVIWHALQNNAATNIDPDDLFHCHVLMTQEKYKQNYDIDV
                     YEGTLSGWLNGYAQDYSGLLCHSQEYANKGADPQKRNIVVMKGQEPFIPLATAGEGIS
                     IPELDDMMGGSRIGVLQLHFYITPDGKIKLPDGRRIDVVVESPGVYTSLRTKMSVDLP
                     SVTE"
     gene            complement(15506..15808)
                     /locus_tag="PKH60_00865"
     CDS             complement(15506..15808)
                     /locus_tag="PKH60_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16634.1"
                     /translation="MNNIEEIYIYFGVDKYAASLDKQVDASKVEQLNRIITKYILSKA
                     IEDVPREKLIIFDTIDFKNESMLVLFLSKHIPNFSKRLINYGNDFRRDFSTTGIVS"
     gene            complement(16163..16483)
                     /locus_tag="PKH60_00870"
     CDS             complement(16163..16483)
                     /locus_tag="PKH60_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HNQ16635.1"
                     /translation="MPEKRKYADRREYMIQAVSKRRLKIKQMAIEYKGGRCTVCGYDR
                     EVSALEFHHLDDTKKDFGLSQRGLTRSWKRTQVELDKCILVCANCHREIHSGKLQLSL
                     ETTK"
     gene            16744..17379
                     /locus_tag="PKH60_00875"
     CDS             16744..17379
                     /locus_tag="PKH60_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16636.1"
                     /translation="MKKLFLTLGILFAGTLVGLVFQSNNEIVRASSENAQEVCPHTGD
                     WTEHYSPNEAGKVIFTVPEGKIVVEVCIKGGSDNSKNGYLKKFTSTSWYQVDGKNCVG
                     ANWREDGKVVARHGDAPENICANISHASFKLSIKPTGEPSPSVEPSPSVEPSPSVDPS
                     PSVEPSPSVDPSPSVEPSPSVEPTPTEGERPTPTITPHSWTRKSGGVGIPS"
     gene            17354..17773
                     /locus_tag="PKH60_00880"
     CDS             17354..17773
                     /locus_tag="PKH60_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16637.1"
                     /translation="MAALEYQVSCENDEVKAEMRFTNDGKPMENVTAEFDYNGNKKTA
                     TSNSDGWAIVIYPRDGENKLTGYADGFYKIEKTVEFPDCPATGGTVLGATTSKGQVLG
                     ATSYAKTGVAADTLMQLTGVLGTLMVSAGALMHAKQK"
     gene            17757..18359
                     /locus_tag="PKH60_00885"
     CDS             17757..18359
                     /locus_tag="PKH60_00885"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HNQ16638.1"
                     /translation="MPNKSKKTKHSKKKKSVKKNSRQYLPAVLVLLGSILLCVSILHQ
                     VWRLRALLPNFQSVAEIQPVSAQRDLGQRPVAIELNGKTISIKPGTESATGWTLDRQS
                     AFHVEQSAYPGEAGNAIVYGHNTSNIFGSLKLLKINDLIEVTTLDGTTRNYQVFDVTT
                     VPPDENGYLFPTNREQLTIYTCAGFLDKDRLIVRAVPDGF"
     gene            complement(18371..19240)
                     /locus_tag="PKH60_00890"
     CDS             complement(18371..19240)
                     /locus_tag="PKH60_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16639.1"
                     /translation="MSKETKGIHLEIQIGDEKFDLMHFAAAYAELTELAVANYVQREV
                     TNKGGVLDQMQNGIEYRKVLIDRVHKHWEESRIGNDAFHKLMSNPETRELGLDLYGAY
                     HDYRRNSVEDEVLPWHESMRLVHLLMANVYGVSLDEQQDLTAEVGEDWVKMMGTTEDM
                     ITDASHRSKAKQEVQVIESYVRQFRDFNTRYFTRVFGKPYGFEDASMKEKVVTTSDPG
                     SAKRQVFFVLLHELYGLDPRNREEVLKPSDDLEIKEKQAEWLAMEAQLVDEIVAHEVA
                     NRFRSVKLSDYVD"
     gene            complement(19331..21109)
                     /gene="dnaG"
                     /locus_tag="PKH60_00895"
     CDS             complement(19331..21109)
                     /gene="dnaG"
                     /locus_tag="PKH60_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNQ16640.1"
                     /translation="MSQIQQVKEAIDIVEIIGQRVELKRAGTYFKGLCPFHAEKSPSF
                     FVSDVMQRFKCFGCAKGGDVFEFLQQYESMTFYEALEFLADKAGIKLEHELRSRDDEQ
                     RQQLLAVLDLAREYYHFVLTKHKAGAVARQYLADRQTTADSIKLFQLGYALPKWDGLI
                     AYLHRKKKFPLELLVQAGLVIKAQGGRYYDRFRDRVMFPLRNPRGQVVGFSGRVLPST
                     ADDQTKQEAKYINTPETLLYHKSKMLFGYSELHQQIRAKKEVLVVEGEFDVITSTQAH
                     VNHIVAIKGSALTADHALLLNRTVERVLLALDKDSAGVEATKRSLQVLKPYDLELRVV
                     EIPEGKDPDDLARTQPKVWREVVDRSMSAYEFILQASLNAHDATSAPGKRKIIQELAQ
                     DFWTIESQVEREFYLQRLAKLLQVQPEILKSDIKHFAEKKRLPDAKPVSTAQVEQIQP
                     SRRGQLERYLTFLLLRGDLSDVSRRLEQLIQYTIESPVLFALVQNQTALSQISNFQQL
                     VDSLAGDLQQQLMELFVDEKMGPILETASVEAEWLRTMAEFVQEANRAKRADLSRQFA
                     ELEAKEKKSPEQEKKLEEILAELARL"
     gene            21173..22723
                     /locus_tag="PKH60_00900"
     CDS             21173..22723
                     /locus_tag="PKH60_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16641.1"
                     /translation="MQYRHWLIVPLLFLVGLLCLKPIQLATADLGRHIINGQQIIENG
                     WQSKILAFNVYSYTHPDYIVENHHWLFGVLTFFVHQLGGFTALSWLGVTLVVSAATIT
                     YLTCRLNSGASQSLLSIFLLIPLLTYRTEIRPELVSMVFLSLSLFLLTLHQKSKLSWN
                     WLMPLLIVMQLIWVNTHIFFIFGIGLPIIFGLFSPKLEKLRWGILASLLILISLVNPH
                     GLAGLLAPLTIFQTYEYQIVENQTLWFMIKRFAQPIYMYALIAFIGILFGIGSQFTVW
                     IKKRNFQHYKKLLPIMALSLIFMVVNFRVVRLLPFTGLILALLLAEVMQTKKWWRWLT
                     QHKDQAAVWSLGLPMGLVGLVLLLSTGLFLPNWSQLGAGLSADNPTAIHFLQNQVNGP
                     IFNNYDIGGMLIYALYPRLSVYTDNRPEAYPEGFFPNDYIEPQENRDRWLALLAQYDF
                     QSIIFYRHDATPWGQPFLIEKIAENDWQPVYVDQSIVVLARNTAMNQELIKTYGLPKS
                     MFTGKPTN"
     gene            22819..23301
                     /locus_tag="PKH60_00905"
     CDS             22819..23301
                     /locus_tag="PKH60_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HNQ16642.1"
                     /translation="MTKLTSALIIIGAGLTLSACSLLPQSSTPVDTEEIEGQTEMMNE
                     NVVIEDATVSTLPSGTAKPLTSDEVSLHSTPDNCWMIVENKVYDVTAFIATQKHPGGD
                     AVLEGCGKEATELFMTRPMGSGTAHSSKAQEMLQQYYLGELVMTGSELDASISGELNQ
                     "
     gene            complement(23313..24035)
                     /locus_tag="PKH60_00910"
     CDS             complement(23313..24035)
                     /locus_tag="PKH60_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16643.1"
                     /translation="MSDAAVTFKNVDLYKDGWNLLKETWKPLLSIGAIWLIIEALAQS
                     SLQYVEQQENSLLSLSVSLASLALSVILQSGFIAASFRIVREKTAEISDLFAQKPVWM
                     NYFLALIAYQLLVALGTILLIVPGIYWALKYSQVLYLIVDKKVGVSEAFNMSGRMTQG
                     MLLKLLGFGLISIPLNLIGLLALGVGVFVTLGITALAYVLLYEKLLQRIGTTQVAAVV
                     KESPPANPTTPVDPPTSASSKT"
BASE COUNT         6987 a         5279 c         4684 g         7163 t
ORIGIN      
        1 aaaaaatatg aaaaaaaaca caccaaacac caaacaaaca aacacggcag gtaagtgtcg
       61 tttaaacaac ataatgatgc tttcatcatg acatactcag ggagaactct caatcaatat
      121 tcgcagatgg ttttatccca caaaccctcg ccagcttgtt gagcctgaga ctccgcttcg
      181 ctgaatagtt cctgatactt tatatctggc ggatacgagc tggcgcggga atatccttcc
      241 cgtactagta actcatttac catcacgcca tctacataaa catatcgaag tagcctgtca
      301 tatctatctg tttcactaac atctttttca agcgtaacca gttttccttc caccaacatg
      361 cgattttttt ctgcggcttg ttcgccaaaa cattcgactg acttggttgg atgatgaatc
      421 tctggagtat caatgccaat atagcgaact ttttggcccg attctagttc gatagtatcc
      481 ccatcaatta ctctaacgac aaaaaattca gactcaacat gttgcgtttt ggcttcttgc
      541 ctaattgcat cttgctgacc caacacttgg cctaatggtt cagcatataa aaaaaatgtt
      601 gcagtggtta caacaatcaa acaagcgaga taaataaaaa taaaaacatt tttctttgaa
      661 atggttagtt taatcagacc taacactcaa ccatcataca acactgggcc ggcgcacttt
      721 cacctgctta tcggcttcag tgagtatgcg agaaatatcc ccatcgtctc catcacaata
      781 aaataccttt cctggaataa tctttgggat actctcgcct ttttcaccaa tgtacacaaa
      841 ttgattttct tcgttagatt gtgtggcgta atcaatccca gcacctagta gcgcactttt
      901 ttcgctctcc ccgaacgcct actcctcata gccattttat tcttgcgtcg cctgcttctt
      961 tttcttgctt gacgtttttg atcaattagt tttttctttt tttctcgtcc aaagctcttt
     1021 ccactactct tgtatgtgtg taattgagca tctgccactt ccatagggtc aagatcaaat
     1081 aatgcccatg ccataaatcc tatatccttt gaactccatg tatgctgatc ttcattggcc
     1141 tttacgtgac actgcttgca aagatatgcc tcattctcga gacttctgat gatatgaggt
     1201 gccaatattg gattctggga acccaagtat gcacctacta aatgatgaac ctctccgttt
     1261 gttacaaatt tgccgcacat ttcgcaacaa ctgttagttc caagtcttct ctctttcttc
     1321 aactctgatg aaaatttgta tggacctcta gtcataaaga cgacatcttt tctctttttc
     1381 caacggtttt atcactcaat ctaatagccg caatactatc agaatatatc agtatgaaaa
     1441 tagggaaaat agttacaatt taaactacat cacaaatgta agcgccttgc ccatcttgcc
     1501 ggcccgacca gtgcgaccaa tgcggtgcac gtagtcatca taggattgag gttcatcgta
     1561 gttgatcacg tgggtgacat cgtcgatgtc gataccacgc gcagcaacgt cggtagcgag
     1621 tagcgcttgg acgtgcccac gtttaaacgc ttctaatgat cgttggcgcg cgttttgatt
     1681 tttattacca tgaattgagg tgactctgac accaagtttt gatagtgtca tttctagctt
     1741 attgattcca tgtttggttc tgccaaaaac aagtactttt ttgaattcgg tttgcaataa
     1801 cataccatgc aaaatatcaa ttttattttg tcctggtaac aacttaacca catcttgttc
     1861 aatatgagta ttaaattcct gcttactcac ttgaattgtg acgggattgg ttagcaattt
     1921 tcttgcaatc gcatctgtat ctgcattcat tgtcgcactg aaaaaagctg cgtgtcgaac
     1981 tggtggcagt tgggcaatta aaaattgaat tgcttgtcta aagccaatat caagcatgcg
     2041 atccacttcg tccaatacaa tgttggtaaa caaggaggtt ttgaacgctt ttcgatcaat
     2101 taaatccttg attctaccgg gagtaccgat aacaaaatga ggatttttct tgagctgacg
     2161 aatttgcgta tctagattgg taccaccaat acagacacag acgccaatcg gcaagcgatt
     2221 ggcgaacgct cggaattctt ccgtgatttg gagcgccaac tcgcgagttg gcgccaatat
     2281 tagaactccc tgattgggat cttggatgac tttgtgcaat aacggcaata aaaacgcggc
     2341 agttttaccg gtaccagtgt tggcaatgcc aatcacatct tttccctgca ttaattcggg
     2401 aatggcttga tcttgaattg gtgtgggtaa ggtatacccg tgccgttgaa tatttgccaa
     2461 taactggcta tctaaaccaa agtcactaaa cgtgttgagt gcttggtaag gttcttcttg
     2521 ctcaggtttc tgtgcagctt gcacgtaccg agaaggtgca atgtatgcac ccatatttct
     2581 acgaggttga attggtccgc gttggcgtga tccgccaaat gaaccatatg aacggggtct
     2641 acccccatac atttgagaca tagtcttcct tttatatttc agcggagaga atcacattat
     2701 tcaccatcag gagaatttca aaccgagact aagtagtata gcccaaaagt cctataagtc
     2761 aagttagtga accggggcaa aaagactcgc atcctgtttg aggcagcgag caaaggcttg
     2821 gatttttaat tgattatacg gataaaggaa tctatatgct agtatttccc tcttaaactc
     2881 atcatcatca agtaataaga tatcaaataa ttcggggggg agatctactt cgcaccagct
     2941 atctctaatt ttccgcctga ccttataagt cattatctct aatccacaaa tcgctctcaa
     3001 aacaaaaata ctcgcaggat ctagtacatc cctcatctca ttttcactct tcataagcat
     3061 ctctggagtg atttcttgac tatgctgacg catcgcttga gcaaatgact tgattgcttc
     3121 atcacaaagt tgttgagctt ccttagaatt ttcaggaagc ccatctttag cattgtctgt
     3181 agcttggttt atggtttgca actgttcaaa taatggatct ggcttatttt tctctccaaa
     3241 ttgctgatac cgatctcgtc ctctctctct ggcaaaaaaa gcatcccccc aagaacttga
     3301 ccgatctcca gcattcgcct ggcctcttct taactttaaa gattgtaata aattttctaa
     3361 acttgctgca agagctggct cacttccact aacgaaatct ctgtcattgt tatacccagc
     3421 ttgatatgat ggttcggtca ttatattcct ttattagtac catacttaac aataaaggtt
     3481 ttgtgcagga tctgtcaact tgtagtaaag tactcagtac ttaatcttaa attaataact
     3541 atgttgtagt attgtattat ttacagcggc gcgagcaact accggatcca aataacgaaa
     3601 aaaatgattg ctcttggata aaccaagttg ctcccatatt ttttgaccat tttctgaaag
     3661 atagtagctt tgtactaaaa aaatatttcg acgaagcatc atttcaactc caattgcatt
     3721 gagtaaggaa ccaattccta ccgcatagtt atccggttca cctatgagta atggacgcct
     3781 tctttctagc gtcattgccg aatttacaaa ccaatagacg ctttctgcgc tgattttcca
     3841 tcctaaattt tctggaggca cgtattgcga tggtggacac ttcaaatcgg taaacacata
     3901 ttgctcacca gtttgttttc tgcaaaaggc taaatcacag tgagcaatca atccatattc
     3961 atcaacggga aacaaagtgc cattgctctg aacatatgca actatggttg ttaattcctc
     4021 aacagaatca gtaattattt cgtacttttt atcccttggg gggataattg tattgaccca
     4081 cacgaagagt ttctctccag taagtgagcg tgtggcaatt tctactggaa ttggactcgt
     4141 agtaaccaac tctaaaaaat ctgcaacttg ttgagtttgc tcacgttttt cttgatatcc
     4201 tgagatacct tcgatgaact tcatgagcta accttcgcta gtcagcaaaa agaaacctga
     4261 atacttcacg aaattgtatc actattggta aaacagcaag cgctttctta tcacgagtac
     4321 taccataatc aaatttatga tactgagata aatttgcatc acctgctaat gttatgggtg
     4381 actcagatta aaaattattg aggattggga agtagagata tcccaaatag gcaccgatga
     4441 acgggagcac aataaatagt accgccgcta acacttttga aagcgtggta acagtagtaa
     4501 gggggttggt ggtttttgca accgattttt tggacatgca ataaatatag cacaacttgc
     4561 tatactgaga taatgccttt ttttacgcaa ctaaaacaat tgttctttct attactttgt
     4621 ctgataattg ctggcttgac tgttgttgca atactgcttt ggctcaaagt agatcgcaca
     4681 gaacagttgc taacaaatag tgctcaggaa acagtctcac ctgaaaattc taaaaattta
     4741 gcaacgtctt caggtcgtct ttcggcgatc gatcaagatc agttggaagc gtcagtttca
     4801 gcgctcgcag ctcgattaga agaagttgag aaaaaccaga aaactataca aagtcaaatt
     4861 gccacgtctg cgagcgctca gcaaaagaac tcaactccac tgtttactaa agaaactctc
     4921 tatcttggtt gggctagcac caacagtcgt gaatggagcg aaacaggtct agaaattggc
     4981 attaattcag cgcattatcc cgcaggcgtc acagtcaaac ttgaggcttc tttgtctatt
     5041 attggcggtg aagcttgggt gcgcttgaaa aataaatcca ctggcgcagt aatttcaata
     5101 tctgaaatat ctaataatac caatacggcc acttggaaac tttcaccaag ttttgcactt
     5161 catactggcg gctatactta cgtcctagaa gcgcgctcaa ccagcggcga aacggcaaat
     5221 atttctgccg ctcgactaat tatagaaaaa tagttagttt gaatgagtga taaattttga
     5281 gttgaaccct ttttctatac tgatatacta tcaaaatgaa tcgtaatgaa tcatggactc
     5341 gatccatcaa ggatgctagc ccatttagat ttgtttatcc agagtttttg acccatcttc
     5401 agtcgcttga attacaaccg aaacatattt ctggttctgt attggttatt ggtcaatctg
     5461 ctgcttgccc cgaaaaaacg ttactagcta caccagaatc aagttttcag gttttaagac
     5521 gcgaggtaac ctcagttttt ttctgtgatt ccgacctttt taccgcacca catattcatt
     5581 ttgataatcc agtaatcaat accattgatt ttgatatcct accccaacca agacaagggg
     5641 aaatctattt tgaaatgagt tcaagttatg actttttaca gcgggcacca acgaattttt
     5701 ttgattcagc cttgatgatt cgagtagaag acactggtac acaaatagca gattatgatc
     5761 tgataaagca attagcgcca catctcaaac ctggtggatt tttcatttgt agcgggggca
     5821 gattcccaac gcatcttaaa tcagaatttt tctcacccct atcgccagtt cgagtggttc
     5881 agttaccaaa ttacagtggt ggttatccgt ttattaacaa catgggagca atcttacaga
     5941 agatgtaact ctctaaaaca gtaactgaga aataattaac tattcttcaa tttatcccaa
     6001 atgtccccag tgcgttcgat tgtgtgaacg ctcagtggcc aaccgacgag ttcctcaaca
     6061 tccaccgctg tcttcaagca ggctgcatgt tttgatagca ctacagctac tccaggattt
     6121 cccttcgcat acgcatgaat ggcctctgta acttcccaac ttaacggcac tggcgcattt
     6181 gcttcaatga tactcaaaat atggtctgag ttaagaccac ctagtgtaac gacttttgca
     6241 gccaattttc cttcaacacc cgtttctctc tctttcatgg tctcccttat catacataaa
     6301 tatctttaat gtaccatatt atatcatatc ggtcgggatg aattaaaaaa cgaggtaaat
     6361 aggacttgga aatcggccaa gtattacctt agtttgattt tatctcaaag aactaatcca
     6421 tcattaaccc atgagtcggt gtctgaagga cacgaaaagg atgggttttg cgccacacaa
     6481 ctattgtcta tgcaccgaac agagctatag ctcgcaacca ttcctggaaa gtaactcaca
     6541 tcgcattcca actgctccgc cattgactcg ctctgacctt cattaaatcc agcagtacat
     6601 ccaatgtcct ccacatatgc ggagtagtca ttccagccac ctttttcaca ccagttatat
     6661 ctgattgaac agtctgcatc taccttgcag taatcttgat tttccaagaa aggaaccacg
     6721 gctggaattt cactgaggac agtaacagaa aataaatcat ttccatagcg tttattgggt
     6781 tgaagctctt tttccgacaa gacataaaaa acaatgtttt gtttttctta aaatgatttc
     6841 ttatatatct cagacgagga ttgagaatat tggcggtaga taactgacca atttcgatta
     6901 ttggaccatg tagtccattc atcaccaaat agtgttttgt acaccattaa ttggagagat
     6961 tcgtcttgtt tcgtacctcc tactttaatt gagtatgccc tcaaagaggt taattctgta
     7021 ttagttgcaa caggattaag attatctgtt tgtgccacag cagtggggct tgaagcatca
     7081 agcacttgtt ctaattcgat cacatctgca ttctgagcaa ttttagataa tttgagttgg
     7141 cgattttgta acatcaaaat aacactcaat aaaacgagaa agataataat tatggaagtg
     7201 ggttttacac ggaaagttga actctttgtc tcctttggaa gttgctgctc aattggggga
     7261 attgcttgtg aatccatgtt tctaacatag gtatgtgtct agacaatgtc aaataattca
     7321 tcctttttcc tgtataattc gtcacttgtg ttactagaat tcatgtatgt tactagaagc
     7381 aaccaaccta gccaaatcta ttggagcgaa gtatcttttc caagacttag cttttactat
     7441 cgtggctgga gaaaaaattg ccctcattgg cagaaacggc tacggaaaaa cgaccttatt
     7501 taaaatcctc agtggcgaag atcatgattt tgaaggcacg gttacaactc gcaaaggtac
     7561 tcgcatagta cttaccaaac aagagcatat tcaggaatta gaacaatcag cgttggagta
     7621 tattttgaat agtgttcccc attattttga gtatcaaaaa ttcctggaag agtacgaaag
     7681 tggtcagaat acaaatttaa accgatattt ggaagttttg gaatatttta ccgaacataa
     7741 atacttcaat ttaagagaag ctgttatcag tagtttagaa gactttggta tatctacagt
     7801 aatggttcac caaccattaa aacacttgtc tggtggtcaa aaacgctatg ttgaaatgac
     7861 tcggatgatg ttttctcagg cggatattct cctgattgat gaaccaacca atcacatgga
     7921 ctataaggga aaagagcgct ttattacatg gatgcgaagt attcttcagg cagttgttgt
     7981 cattactcac gatagagatg tacttaaaaa tgttacaaaa ataattgagt taaaagatca
     8041 aaaaatgcat ttttttaaag gtaattacca cagttatata aagcaaaacg ccacgcaaac
     8101 actcacatca gtaaaactct acgaagatca actcagtcgc ttaaaagaag ctaaaaagaa
     8161 agttgagtgg ggactccaaa tgcgtgctaa gagtaaagag tggaagattc gctacgatca
     8221 ctggctaaga gattatgaaa aaattaaagc agcaactatc aaaccagctt tttggattga
     8281 tcaaaattca atcgaaagtt tggacaaaaa agtagttgat tcgtatgata agtttaaaga
     8341 aaaaaacatt aagatttcag gtactggtga cgtaaaaaga ggcggagagc tggtttctgt
     8401 ccgacaattg gcagttgggt atgatgaatg cctcttctct gaaatcaatt ttagcctcaa
     8461 agaaagtggt cgcgttttta taaaaggtag gaatggtgct ggcaaaagta ctttggtaaa
     8521 gactttgatt gccttatcag aaaaacaacc accaactgcc aatcttttgg atggtgaaat
     8581 tacatttggc aatgatttga gaattggcga gtaccaacaa gaaattgatg gaaaatatct
     8641 gtcactacct ctagttgaag ccattcgcgt tattcatgag gaaaaaaagg tggcaattga
     8701 tgaaagaaaa gtctatagcc tgctcgccca atatttattt gacaccaaac tagatgcaca
     8761 ccaaaaaatt gccaacttat caggcggtca aaaagcgcgg tttcaattaa tcaaaatgtt
     8821 tattggtgaa ccaaatttat taattcttga tgagcctact aaccatttgg atttgccctc
     8881 aattgaagag cttgaaacca tgctcgaaaa ttttcctggc gcaattctgt atatttctca
     8941 tgatacctat ttcatcaatc gccttggtgg agatgcgatt caattatagt gcggcccaag
     9001 ccactgctta ctaccactta ttctggtccc tatagatcta gcaagcagta gcaaatactg
     9061 cagtgttgta cgaagaacgc aatagtcaga aagtgggaat caaaaagctg gatgcggcaa
     9121 acacagctca atttagaatc cattcacgag cttgacactg cttcattgag cgtgatattt
     9181 ctgtcgacct cagcccccag cgcatatttt aaagcagaag gtaaacttga aaattcatac
     9241 gggtggtcaa aaatgaaaat acaccttgtg tatttgctga ttctagaaag ttctgcagct
     9301 aaacagtatt tagtatgtga tcagcattaa cccaaacgta atactccgga tgttgttgct
     9361 taaatgcact cggaaaacct tgactattca ttgctatatc gactactctg gctatatctg
     9421 cctctaaggc aggtgaattt aaagaaagaa caattggctg tgcagcatct aactgagaaa
     9481 gatggttctt aatacgttct accaaatctt cagcatgtgc acgagagctt accgtcacag
     9541 aaatagaact gccatcagca atcatctcgg ctataacttg aatatctctt ggtctgtatt
     9601 gaaatggata gctatctgtg cgactgggag caacttcagg catagagtcg attatacaag
     9661 atttttcaca gacacaaata aaaaattgac tttacaggcg cccaatccac tcaataattt
     9721 ttctgcaaac tcgtttaata aagtgttagt cgctgttact caaaattgcc tcaccctatt
     9781 ggactaaggt ggatcatttc tgataagcta gagcgtatga ataatcaaga ctttcctccc
     9841 agcaacgatc agttggaaaa tgaactgctc ctccagccac ctaaaaatca gtcaaaaatt
     9901 acacttgttc ttactacttt gcttatcgtt gcagtgggag ttgccattgc acttggctac
     9961 caactcttca ctcaaaacca aaaaattggt ttacaaccct caacaccaac tacccctcct
    10021 acttctgcta ctctgtcccc cacagccacc gcacctgagt cgcgaacata cactgacccg
    10081 aagagtagat ttagtttttc ttatccacaa agctggcaag tgaatgacaa atcaagttcc
    10141 aaccaagatc ctcagttaac gttcaccaac atccccgagg gtcacacaat caatgttagt
    10201 gtttggcgag ttactggatt tggttattgt tatatatatg gtgaaaaaca gacaattgta
    10261 gttggtacta aaaacgcaga aacagcagat ggcgttggac catcagaaat gtgtgataaa
    10321 cccgaagagt atgccaatcg tgggaatacg tatgtgctga taccacttga agatacaact
    10381 gacggcctac cactgaatca actacacata agctatgact acccgctaag tgacaaaagt
    10441 atcgccaaaa cctatctcaa tcaaatcctt tcttcagtta aacttactac taatttgacg
    10501 gcaaataatc aaccaacaac ctcaattctt gaagttgcga aaaatgttca gcagtacgac
    10561 gataagggaa atactatccc aggggtagca tctgtggaca tcatcattga cttaggagct
    10621 aatgcatcag tcgtccagac tgaaagaaaa attaagggca tggatgtata tgagcagaca
    10681 gtgaaaacag caaacagcga gtattcattt accagtgcag ctggaggatt gtgcccttgg
    10741 gaggatgatg gaagtggatg tacatatacc gacgaaaaaa taaataaatt tggagaagat
    10801 gcaaaagttc gagtttggag agacaatcaa agaggtgttt ttgcgttaaa ctttcaatca
    10861 ctagaaattg gaaattatta tgttgaccat ttcgaaatat ttaaacgctc tccaaatgaa
    10921 tcatttactg ctagcgagat tacaacttgg aaaaattggg ttatgaatat aaaaacaatc
    10981 ccgcatattt gaactttcaa ccttttcgtg tcaaatccct ttcgtccttt tggactcatc
    11041 agtacggaca cacatccgta gaggctaaaa gctgcggaac gggtgttccg cagcttcagc
    11101 acttgtatca aaaaagttgt tgttacggcc ggagtaattc cggctgctgg ctgaggttgc
    11161 catcggcgtt catgaaaggc gggacttgct caccgtacat gagaacccat aggatcctcc
    11221 aatcagatgt tgagtaggca gtcagtagct tctgcgccac ctgcacgcga ttgtccgcgt
    11281 ccaaaagcgc ctgctctcgg gcaaacgctg cccgctccgc cttcaatttg ttctctgccg
    11341 ccaggttttt ctgttgctcc tgcaacagcg cagcctggtt ctgctcgacc gccaactcct
    11401 gctcccgttg gagctgagcc tgtctccagg cctcatcccc aaaggtaatg ctgcggacca
    11461 gaacttggtc cacaatgatc ggagtggctg ctgttgaagc cttaagcggc tgtagataag
    11521 cttcctgaag cgcctctcta ccagcctgtg ttttaagttg ttcgatagta aatggtgaac
    11581 tggcagcccg acttcctcga agtgtagaac ccacaaagtt ctgtactgct acttctaaat
    11641 cttgtaactt ccagagtgct tgtgggtcag ttgtatcgaa gtggaacagg acatccacat
    11701 cgactgttgc ctccacttga ctgccggaga cagatgctcg cgtgatagaa gtttcgcact
    11761 tgaacgattc acaagcattc tcgtcaggca gcagttctga cgccaacgtc cgatagcgaa
    11821 gactgccggt agggattcgt atcacctctt ccagcggaac agcatgacga ccaggttgca
    11881 aagcctggta agttccgtct ccggtactgt ggacagcaat ccagcctgtc gtgatcacag
    11941 tcatatcacg aaagatggtg atatctcctt ccgctaggtt ggggtatggc ccgggcaaca
    12001 ggttcctcgt gccatcccaa cccagctttc cttcaggaac gataagaatt tttttgagct
    12061 cataaccctt gtcgttcaca tcgtaccgat tccccgccgt tatcaccgag gtgtagcggg
    12121 gttcgttgcc gccaggcaga aaagctggat tcagctttac cgctacctgg cccgctgaga
    12181 caataggttc cgtcccgcta catgccgaga gcagtaccac caccgtcatg ctcagaacga
    12241 gcaaccatcc aaacttccta aacgtagaca tgtttgttct cctaattgaa aagttgaatc
    12301 ggtcttcgat ttgtgtcaac ttgtacctcc atgggtactg ggccaagaca cagagtcgcc
    12361 caatcagttc aagactgacg aaaaaggcac atataatata tgcattcatt gtcagtcttg
    12421 agtaaagtga aaattaaatc tctataacaa catactttta tgatattaaa gtgctacaaa
    12481 agtgaaaaat cgccaaacaa tctaccgcgt tggtagcatc tggcttcctg atcatttttg
    12541 atgcttttat aataacatca ttcaagagta ttgtcaatgt tataggacta ttcttcacga
    12601 tcaggtctac cccgcctttc gcgaaagtcg gggtcactat acgaactaat ctgatctgaa
    12661 gaggaaaagt gtggaaactt gttatgggac gatcctactt ttcaaactga tatgtgcaaa
    12721 caggtgattt tggatgcttt ctttatcaga tgcctgctaa tataccaagc tatgtttgac
    12781 aataaaaatg ttacaattag tggttagcaa gcgaaaggaa gctatgggcg ataatgaatt
    12841 aggtgttcac tccgttgatg gtgttttggt aaatcgagtc aaacgcgtta atttcgatga
    12901 cctgctttat gggaaaaggg agctaatgcc agagtcagca ggcctcccta attggctgag
    12961 acggaacgca ccaaactttg aagttttaga ccaaatattt gatcaattcg ttgagagagc
    13021 caatggtgct gatattgaga ttgacgatct caaaactatc cactttctga tctcatacga
    13081 tgtatcgctg ttacgaaact tcgaaagcgg gatagctatg gcgagacact atggtgaatt
    13141 ggaaggcttt gatgatcagc cacttgtttc tctaattgag acagcaatct ccaagcgcgt
    13201 cgcttttctg gaaaccttga caaaccaaat agatactttg ttgtcagcgg agaaaaatgg
    13261 tgaagttgaa atcaaataat tatccataac aaaacttgca cataaactga cttagagcta
    13321 cagcaacaat tcaatccagg atttaactag gtaacaactc ttttgctctc ggcgagtctg
    13381 tcaagcagtc tgcctagaag cgcctcagct tgtgtgctat ctgaagtcac taattcccta
    13441 aaagaaccag cgccaaatac tccttcaatc tttttggcaa aagcaagtgt atgcttttta
    13501 agtcttgcac cttccattaa tttttccata ccttgaattc ctgaaaaatc cctaaacaac
    13561 ttctcatatt tggctctgaa ttgggggctt aggatttcaa ttaaatcact tccaccattg
    13621 atcaaccaat caaccaatac ataggagtcc ttataaggta agaaggcagg tattgctcct
    13681 ccctcttcgt ctatacctct caaataaata agatcttttg ctctgtcatt aaatccttca
    13741 agattatttc gatagaattg aaacatgttc cctgatatcc tgaagtgtct agcgataatt
    13801 gtatatgctt caatttgaaa tcgtgtagca agaccttctt ctacagctcc aggttttttt
    13861 gcgtaattca gcccagaact ctctctagcc actccattcc aaactggtgc aaaaattgtc
    13921 ggagcggtag catgatacat ttcatgagcg ataattgatg ttcttaagag ttcattccag
    13981 tccctatact cctctgtctc taaagtaggc gaatattcaa aaacaagtat agatccatca
    14041 tcagatgtag ctccaacaaa tggaattcca tgtcctttat tgctggtacc gggagccata
    14101 aacacatgat ttggcaacaa catggcctct tcaagattta agttagaaaa atgagcggtg
    14161 agcagttgtt taacttcccg aagcacatca gaatcatcaa tatctatttc tatcatacct
    14221 tcttcttgcg atgcttcaac tatttggtct gcaacatctc tgattccctt tatccaagga
    14281 gaactctcag gcactcccac gaaccgaaga tgaggattta aagcggcgtt ttcaaccata
    14341 taaacaataa gctacttcta atttggtttg tttcaacagc aataatagta acagataatc
    14401 ttggcaattg ataatcgtca tcctactggg aggggtgtct aaaggctatt ttatattgct
    14461 atatctatct atgtgaaaaa ccccatccca acagacgtgt gcccgaatgg gtatcactac
    14521 acgaacttac taacacaaat aaaacctgta cccgttgtct gttgaggtta gttttccatt
    14581 tacaaaccaa attaggagtt gttttaggta attcttctca tccaattttt gactatttct
    14641 ctgatttgac ttaaatgcga aggatctcta aatgtatgtt ctgccccttc aactattttt
    14701 agcttataca cactattctg tcacactagg taagtcaacg ctcattttcg tacgcaaaga
    14761 cgtgtatacg ccaggggact caactacaac atcaattcgt cttccatctg gaagcttaat
    14821 tttaccgtct ggggtaatat aaaaatgtaa ttgaagtacg ccgattctac taccccccat
    14881 catgtcatcc aattcaggaa tggatattcc ttctcctgcc gtagccaaag gaataaaagg
    14941 ttcttggccc ttcatgacta caatattacg tttctgtggg tccgctcctt tattggcgta
    15001 ttcttgagaa tggcacagta atcccgaata atcttgagca taaccgttta gccacccgga
    15061 taatgtgcct tcatatacat caatatcata gttttgctta tatttctcct gagtcattaa
    15121 aacatgacaa tgaaataaat cgtcagggtc aatatttgtt gctgcgttgt tctgcaacgc
    15181 atgccaaatg acttttacca agtcttcttt cagctgtgct ggaagattga tagcatatgg
    15241 tgctagttcc ttatagggct caaaaataac tcgatcgggt gaatatgtgt gcgaagattc
    15301 ttctgacaaa gcgattggcg ttcgagcaaa ctcagtcgca gatgcgtact gtggtggcgg
    15361 atttccctgt gtctccacag tggagcacgc agagagaaac agtgctccaa caagcacggt
    15421 cattgctagt ctagtaacaa tacgcttttt gatatttgac ttggttgcaa ttgcaaagtc
    15481 aacttgttgc tctattgcat ccagattagg agacgattcc ggtggttgaa aaatctcttc
    15541 tgaaatcatt tccatagtta ataagtctct ttgaaaaatt tggaatgtgt tttgataaga
    15601 ataatactaa catactttcg tttttgaaat cgatcgtgtc aaatatgata agtttttctc
    15661 ggggaacatc ttctattgcc ttcgacagaa tatattttgt gattatcctg tttaactgct
    15721 caactttcga tgcatccact tgtttatcaa gtgaagcggc atatttatct actccgaaat
    15781 atatgtaaat ctcttcaata ttattcatgc ctttctatta tattggttat catgggtatt
    15841 tgcagaatca atcaatccgc gtaggttatt aagttatttg cagtgaatac gatctataga
    15901 actagtattt tttagcaacc cacactgatg gctcccccgg taggattcga acctacaacc
    15961 cttcggttac actgattcat gcatttctgc gtggcgtgga ctatctcatc accaatcctt
    16021 gttaggatta cggtgtcggg cgcttcttcg tttttacgaa gtggtgtact cacctagtct
    16081 ctgaaccttc ctcatcgaaa cgaggcttgg ctgcggatta ccatatcatt tagatttagg
    16141 cttcccgcaa ttcacccgat gttcatttcg ttgtttccaa cgaaagctgc agttttcctg
    16201 agtggatttc tctgtgacaa ttggcgcaaa ccaaaataca cttatccaac tcaacttgag
    16261 tacgcttcca cgatcgggtt agtcctcttt gtgaaagtcc aaaatctttt tttgtgtcat
    16321 ctagatgatg gaactccaag gcactaactt cgcgatcata gccacataca gtacatcgac
    16381 cacccttata ttcaattgcc atttgcttga tttttaatct acgcttactg acagcttgaa
    16441 tcatgtattc tcttcggtcg gcgtactttc tcttctcagg catttttcga aaaataccag
    16501 actttattta gtgtgtctac agccgaatgc tctgccattg agctacaggg gataataatg
    16561 tgcgcgctgt taaatggggt tcattcctct taacaacagt agccgattat atcgttatat
    16621 atagaatttt cacaactagc ggttctttaa gtttaattgt tgatccatac tgatgcagta
    16681 tgttataatt tgctactttt ttacagtagt acgttttttg tacaaataaa acaaaggtgc
    16741 taaatgaaaa aactattttt gaccctcggt attctgttcg caggtacact tgtcggattg
    16801 gtttttcaat caaacaatga gattgtgcgg gctagttctg aaaatgctca agaagtgtgt
    16861 ccacatacag gagattggac agaacattat agtcctaatg aagctgggaa ggtcattttt
    16921 acagtacctg agggtaaaat agtagttgag gtttgtatta agggcgggag tgacaattca
    16981 aaaaatggtt atctgaaaaa gtttacctca actagttggt atcaagttga tggaaagaat
    17041 tgtgtcggag ctaattggcg tgaagatgga aaggtggtag ctagacacgg cgacgctcct
    17101 gaaaatattt gcgctaatat ctcgcacgct tcttttaagt tatccattaa accaacggga
    17161 gaacctagcc cttcggtaga accaagccca tctgttgaac ctagcccttc ggtagatcca
    17221 agcccatctg ttgagcctag tccttcggta gatccaagcc catctgttga acctagccct
    17281 tcggtagaac caaccccaac cgagggtgag cgaccaactc caaccatcac cccccactcc
    17341 tggaccagga agagtggcgg cgttggaata ccaagttagc tgtgaaaatg atgaagtcaa
    17401 agcggaaatg cgttttacca atgatggcaa acctatggaa aacgtcactg cagaatttga
    17461 ctacaacggt aacaagaaaa ccgcgactag caacagtgac ggctgggcca tcgtcatcta
    17521 cccaagagat ggtgaaaaca aactaactgg atatgcagac ggtttttaca aaattgaaaa
    17581 aaccgtggaa ttccccgact gtccagctac tggcggtact gtcttaggtg ctacaaccag
    17641 caaaggtcaa gtactcggtg ccactagcta cgccaaaact ggtgttgctg cagataccct
    17701 tatgcaatta actggagttc ttggtacact gatggtcagt gccggagctc tcatgcatgc
    17761 caaacaaaag taaaaaaacc aaacattcga agaaaaaaaa gagtgtcaaa aagaacagcc
    17821 ggcagtatct gccggctgtt ttagtactcc ttggtagcat cctgctctgc gtttctatct
    17881 tacatcaagt ctggcgcttg cgggcgctgc tgccaaattt tcagtcagtg gcggagatcc
    17941 aaccggtatc agctcagcga gacttagggc agcgccccgt cgccattgag ctcaatggca
    18001 aaactatctc gattaaacca ggtactgaat ctgcgactgg ttggacactc gatcgtcaat
    18061 cagctttcca tgtagagcaa tcagcgtacc ctggagaagc tggtaatgcc atcgtctatg
    18121 gccacaatac atcaaatatt tttggctcac ttaaattgtt gaaaatcaat gatcttattg
    18181 aggtgactac gctcgatggc acaacgagaa attatcaagt ctttgacgta actactgtgc
    18241 caccagatga aaatggctac ctattcccca ccaacaggga gcaactgaca atctatactt
    18301 gcgctggttt cttagacaaa gatcgattga ttgtcagggc ggtgccagac ggtttttaaa
    18361 ataacatttg ttagtctacg tagtcactca acttaaccga tcgaaaccga ttggccactt
    18421 cgtgagccac gatttcatca actaattgtg cttccatcgc aagccactct gcctgctttt
    18481 ccttaatttc aagatcatca cttggcttca acacttcctc acggtttcta ggatcgagtc
    18541 catacagctc atgcagtaac acaaagaata cttgtctttt ggctgagcct gggtctgaag
    18601 tagtaaccac cttctctttc atagacgcgt cttcgaatcc atagggttta ccaaacaccc
    18661 gagtgaaata tcgtgtatta aaatctcgaa attgccgcac atatgactct attacttgaa
    18721 cttcttgttt agctttagag cgatgactag cgtcagtgat catatcttca gtggtaccca
    18781 tcattttcac ccaatcttca ccaacttctg ctgtcaaatc ttgttgttca tccaaagaca
    18841 caccatacac gtttgccata agtagatgta ctaaacgcat agattcatgc caaggtaaca
    18901 cttcatcctc aactgaattc cttctatagt catggtaggc tccatagaga tctaagccta
    18961 attctcgcgt ttctggattg ctcatcagtt tgtgaaaagc atcgttacca attcgacttt
    19021 cctcccaatg tttgtgaact ctatcaatta acacctttct atattcaata ccattctgca
    19081 tttggtccaa tactccaccc ttgttagtca cttccctttg aacataattg gcgacagcaa
    19141 gctcagtcaa ttcagcatat gccgcagcaa aatgcattaa gtcaaacttt tcgtcgccaa
    19201 tctgaatttc caaatgtatt cccttagttt cttttgacat ttttcctctc ttgagcagag
    19261 catgttaaag taatcgaagt atatcacgaa tacagcatga gtatgtaaaa actaggtgga
    19321 tacatttaaa ttatagtctt gccaactctg ccagaatttc ttctaacttt ttttcctgtt
    19381 cgggtgattt cttttcttta gcttctagct cagcgaattg tcgtgagaga tcagcccgct
    19441 tagctcggtt agcctcttgc acaaattctg ccatcgttcg tagccattct gcttccacac
    19501 tggccgtttc caaaattgga cccatttttt cgtcaacaaa taactccatc agctgttgtt
    19561 gtaaatctcc tgccaacgaa tctactagct gttgaaagtt actaatctgg ctcaacgcag
    19621 tctggttttg cactaaagca aataaaactg gcgactcaat tgtgtactga attaattgtt
    19681 ctaatcgtcg actgacatct gacaaatctc cccgaagaag taaaaaagtt aaataccgct
    19741 caagctgacc tcttctagaa ggttgtattt gttcaacttg agctgttgaa actggtttgg
    19801 catcgggcaa ccgtttcttt tccgcaaaat gtttaatatc ggacttgaga atttctggtt
    19861 gtacttggag tagcttggct aagcgttgga gatagaattc gcgttcaacc tggctttcaa
    19921 ttgtccaaaa atcttgggca agttcttgaa taatctttcg tttaccagga gcgctcgtgg
    19981 catcatgggc attcagactc gcttgcaaaa taaactcata tgcactcatc gagcgatcca
    20041 ccacttcgcg ccaaactttt ggttgtgtac gagctaaatc atcgggatct ttgccctcgg
    20101 ggatttctac aactcggagt tccaaatcat acggtttcaa cacttgtaat gaacgcttcg
    20161 ttgcttcgac tccggctgag tctttatcta aggccagtag tactcgttca actgtgcgat
    20221 tgagtaacag agcgtgatcg gcagtcaacg cactgccttt gatggctaca atatgattga
    20281 cgtgagcctg ggtagaagta atcacgtcaa attcaccttc taccaccaac acttcctttt
    20341 tggctctgat ttgttggtga agctcgctgt aaccaaacaa cattttactt ttgtgataga
    20401 gcaacgtttc tggcgtattg atgtatttgg cttcttgttt ggtttgatca tcggcagtac
    20461 taggcaaaac tcggccggaa aagcccacta cttgtccccg aggatttcgc aagggaaaca
    20521 tgacgcgatc gcgaaatcga tcgtagtacc ggccaccctg agccttgatc accagaccag
    20581 cttgaactaa taattccaac ggaaatttct ttttgcgatg cagataggca attaagccat
    20641 cccatttggg tagggcataa cccaactgaa aaagcttgat tgagtcggca gtagtttgcc
    20701 gatcagctaa atactgtctg gcaacggcgc cagccttatg tttggtcagc acgaagtgat
    20761 agtattcccg cgctaaatct aagactgcta agagttgctg gcgctgctca tcatctctgg
    20821 aacgtagttc atgctctagc ttaataccag ctttatctgc taaaaattcc aaggcttcat
    20881 aaaacgtcat gctctcatac tgctggagaa actcaaaaac gtcaccgccc ttggcacagc
    20941 caaagcattt aaaacgctgc atcacgtcac tcacaaaaaa tgagggcgat ttttcggcat
    21001 ggaaaggaca cagtcctttg aagtatgtgc cagctcgttt taactccact cgctggccaa
    21061 ttatctccac aatatcgata gcttctttaa cctgttgaat ctgcgacatg ttgcctcaag
    21121 tcgtattgta cacgatgata cttagttcag aagacgctac aataaccaaa tcatgcaata
    21181 tcgtcactgg ttgattgtgc ccttgctgtt tcttgttggc ctgctgtgct tgaaaccaat
    21241 tcaacttgct actgcggact taggcagaca catcatcaac ggacaacaaa ttatagaaaa
    21301 tggttggcag agcaaaattc tggctttcaa cgtctattcc tacactcatc ctgattatat
    21361 tgttgaaaac catcactggt tatttggtgt cttaactttt tttgtccatc agctcggcgg
    21421 ctttaccgca ctgagttggc tgggggtaac cctggttgta tcggccgcca ctatcaccta
    21481 tttaacttgt cgattgaaca gtggcgcgag ccaatcatta ctcagcattt ttctcttaat
    21541 tcccctactc acctatcgta cagaaattcg gccagaacta gttagtatgg ttttcttatc
    21601 gctctcactt tttttactga ctttgcatca aaaaagtaaa ttatcttgga attggttgat
    21661 gcccctgcta atagtaatgc agttaatctg ggtcaacact catattttct ttatctttgg
    21721 catcggtttg cccatcattt ttggcttatt ctcacccaaa ctagaaaaac tacgctgggg
    21781 aatcttagcc agtttattaa ttttaatttc gctggttaat cctcatggcc tagctggttt
    21841 attggcacca ctgacaattt ttcaaacgta cgaatatcaa attgtcgaaa atcaaactct
    21901 gtggtttatg attaagcgct tcgctcaacc catttatatg tatgccttaa ttgcgtttat
    21961 tggaatctta tttgggattg gctcacaatt tacagtttgg ataaaaaaaa ggaattttca
    22021 gcattacaaa aagctactac caatcatggc tttgagttta atctttatgg tggtgaactt
    22081 ccgggtggtg cgtttattgc catttaccgg cttgatcttg gcactgctgt tggctgaagt
    22141 catgcaaact aagaaatggt ggaggtggtt aacccagcac aaggatcaag ctgcagtttg
    22201 gtcactgggt ttaccaatgg gcttagttgg gttagttttg ttgctgagta ctggtttgtt
    22261 tctgccaaac tggtcgcagt tgggtgctgg cttaagtgct gataatccca cagccataca
    22321 ttttttacaa aaccaagtaa atggaccaat ttttaataac tacgatattg gaggaatgtt
    22381 aatttatgct ttgtatccgc gattgtcggt ctataccgac aatcgccctg aggcttaccc
    22441 cgagggattt tttccaaatg actatattga gcctcaggaa aatcgggatc gctggctggc
    22501 cctactggcg cagtacgatt tccaaagtat tattttttac cgacacgatg ctacaccctg
    22561 gggccagcca tttttgattg aaaaaatcgc tgaaaacgac tggcagcccg tctacgtgga
    22621 ccaaagcatt gtcgtgctag cgcgcaatac agcaatgaac caagaactaa tcaaaaccta
    22681 cggcctgccc aagtcaatgt ttaccggcaa accaaccaat tagttaagtt taatcaaaac
    22741 ttttttatac ttgtcaaaat gccgttgttt ctgttacatt tccaatcatc ttcttagtac
    22801 tactgaaagc aaatgcatat gaccaaacta acttcagcac tcattattat tggagcaggt
    22861 ttaacacttt cagcgtgttc tctcttacct cagtcttcca cacccgttga tactgaagaa
    22921 attgagggtc aaaccgaaat gatgaacgaa aatgtagtta ttgaagacgc aactgtttct
    22981 acgctaccct caggaacagc gaaacctctc acgagcgatg aggtttcgct ccatagcact
    23041 ccagataatt gttggatgat tgtagaaaat aaagtctacg acgtaaccgc cttcatagct
    23101 acacaaaaac atcctggtgg tgatgcagta ctcgagggtt gtggcaagga agccaccgaa
    23161 ttatttatga ccagaccaat ggggtctggc accgcgcact ccagcaaagc acaagaaatg
    23221 ctgcaacaat actatcttgg ggaactagta atgactggct cagagttaga cgccagtatt
    23281 tctggagagt tgaatcaata aatcaagatt aactacgttt ttgaggaagc agaagttggt
    23341 ggatcaactg gcgttgtagg gtttgccgga ggtgactctt ttaccaccgc ggccacttgg
    23401 gttgttccga ttctttgtaa cagcttttca tacaacaaca cataggctaa agcagtaatt
    23461 cctaaggtga caaacacacc cacgcccaag gctaataacc cgattagatt tagcggaatg
    23521 gaaattaacc caaatcctag taatttcaac agcatccctt gagtcattct tccagacata
    23581 ttgaaggcct cagacacacc tactttttta tcaactatca aatacaaaac ctgactgtat
    23641 ttaagagccc agtatatccc tggcactata agtaagattg tgcctagcgc cactagtaat
    23701 tgataggcaa ttaaggcgag aaaatagttc atccaaacag gtttttgtgc aaataaatca
    23761 ctgatttcag cagttttctc acgaacaatc cgaaatgatg cggcaatgaa tcctgattgt
    23821 aagatcacgc ttaatgccaa actagccaaa cttaccgaca aagacagcaa agaattttcc
    23881 tgttgttcaa catactgaag tgaggattga gctaatgctt caataatcag ccaaatagct
    23941 ccaattgaga gcagtggctt ccaagtttct ttgagaagat tccagccatc cttatataga
    24001 tcaacatttt taaatgtaac agccgcgtct gacatactgc ctttcattca agagttactc
    24061 tgaattaggc acgatacttt ccggaatgga aaggtaagtg tgaaagttag aca
//
LOCUS       DAOQZP010000008        23641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_2507_length_23641_cov_6.126601, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000008 DAOQZP010000000
VERSION     DAOQZP010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 23641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23641
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2507_length_23641_cov_6.126601"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3056)
                     /locus_tag="PKH60_00915"
     CDS             complement(<1..3056)
                     /locus_tag="PKH60_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16644.1"
                     /translation="MFRLRLITLVVFVLFSAVLLHPNFASAYDLTDLKADFEFLMSQT
                     PVESALLFNVFGTNPDPNFQKHQLTDFQLNELCDGDCGAAGLGVNYATYYKGGNYEKA
                     RQFKMKWTLEIARASTSPQTVADVINNEQVDNVIIRIGTTQGSEGFDNPADYANFLRA
                     VAGLTSRPFYAIAGPNEPDIEHWAAPTCNAPNGSSPPAEKAAFYDCVGPKLATYMNTL
                     VGAGLPSNVRLLSPAFNLTSFMFNDDTGRPDGIPRAMRRAGANFGALYAVAGNIYPVN
                     DTMQNIWTNHVAGVVGELGRPVVITETGPWTSISELYAGYDISIYDAYNAEQDQFYIH
                     PIKGLNRSLGSSGGVDLNLKTIRDDLIDQGYEARCATPGFKIELTQDGKDWMADYVEQ
                     FPPGAVFGGYENYYIRTRTSGPPGIGNYVRSWLAVDYREVQTPVFRDVSDKRFLTTSL
                     EEFFGFKDTVIQENTKAELNSAAINSLLTNQQRCEASVRILLKQEEMCNKLVNPSACA
                     LYSRPVPQTNYTIKTMLDEYKALASVPHPDIQPVPLPAYTDPITIEAVCDTVVSTDWN
                     ETARQGFLFTPLTIDRAYRLAFLVTTIELKHPTYASMFNFYIHPNEGPVGGPSKPDDA
                     ILVNAFKVPDILTTTQEEVVGLSSSGNTPWMDSSKLTRNSLITRKIQDKYVATASAKK
                     AELQTAANWYDTHAQTSSDEIFCLVGASTSGIGPPECKDELGKAVIDIVNAQTSSRVT
                     TKYNNKIDPECPELVKEPATSVLDFGGFGTEEFVKNPPAQLLYTQEFGAELLHNIFEV
                     PDKNQDPTGELDATHQVEPKGESNPAFAETWKPGDPDDGENPLGSPRDWGLKSLFHVT
                     DRTYPSFPYNGCCDEREVKHFLVYPVGYDMETVQEVLAGTFFTTDQIESLTTSAEEFD
                     RIQLSGDSIEFTGGTRDHTFSDDRIEPPNWNGQPDMIPVGQGGGVYDIDSCQKVYQQV
                     CSGTDPDTGLPTGCQDVLIGYNLPCERTFGWDILQKGPSLSAGLVGG"
     gene            complement(3100..3525)
                     /locus_tag="PKH60_00920"
     CDS             complement(3100..3525)
                     /locus_tag="PKH60_00920"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16645.1"
                     /translation="MKPARNHLVALSLFVALVSLSFAIPAQAQAQTRTWSGVCLAGSS
                     GDVPTIQGLECLIANIFTVIITLIGLGGFVMFILGAFKWLTAGSNSKNIDSAKRTFTY
                     AILGLVLALSAFIIINLIADFTGVTVIKTFRIPTSAINW"
     gene            complement(3602..4009)
                     /locus_tag="PKH60_00925"
     CDS             complement(3602..4009)
                     /locus_tag="PKH60_00925"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16646.1"
                     /translation="MKKTLTKIVAGASTAGTALFLTASSAFAAVSIGTVSVPAKGFAT
                     NLGGVISSLLSIVMALAALAVFLYLIWGAFEWITSGGDKGKTESARNKITAAIIGLIV
                     LAAAYAILMLLLQILGFDNLEDAFGGIRQINTR"
     gene            complement(4142..4633)
                     /locus_tag="PKH60_00930"
     CDS             complement(4142..4633)
                     /locus_tag="PKH60_00930"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16647.1"
                     /translation="MLQSNRILSTITTGLLVVSLFVARPFSALAANTIDFDITEEARN
                     QDLPTADIPGASASSNVFKQAFADLIGSILSIVISIAFIMLLIYLIWGGFAWITSSGD
                     KGKLDEARNRMSTAVIGIIVLSSVIALFMLVQQMLGICVLDFWATACVAPPAALPVPT
                     PTP"
     gene            complement(4638..4997)
                     /locus_tag="PKH60_00935"
     CDS             complement(4638..4997)
                     /locus_tag="PKH60_00935"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16648.1"
                     /translation="MKIIETAQAAITNPAVGNLGNDPDKAADGSLFLSQIVRYWNNII
                     SIGGLLLLLFFVWGALQWITASGDKTKLEAARNRMLQAAIGLLILVSSFVIIGFVGNL
                     LFGDTFQVLNFKFFTPE"
     gene            complement(4994..5425)
                     /locus_tag="PKH60_00940"
     CDS             complement(4994..5425)
                     /locus_tag="PKH60_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16649.1"
                     /translation="MRNRLKSVFYLLGILVVLASPVRVLAQFGEGIVAPFDPDNEYVK
                     DLEVGSTAFTTLEKIVSNILALLTVLGGLVFITYFMLGAISWITSGGDTAKVGKARDQ
                     MLQGALGLIVLTMMYALVGLIGSIVGMNILNPAEVLETIVP"
     gene            complement(5463..6041)
                     /locus_tag="PKH60_00945"
     CDS             complement(5463..6041)
                     /locus_tag="PKH60_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16650.1"
                     /translation="MKRLTFLFILPWFISFFFLFFGSISPVNADTCISPPNHNCLNGC
                     TSIGQCGPNNKVCILIGPNTVAPNGADCGSAIIGGISAPQGVGVYNCDTGNCTNIGIF
                     SFISVALRLFTVICGLFMFFNFLWAGYALITKAGDTKAFTDVRERLQYAMIGLVIIVA
                     AYMLAAIIGLVFFGNAGFILKPDISQYGATAP"
     gene            complement(6038..6487)
                     /locus_tag="PKH60_00950"
     CDS             complement(6038..6487)
                     /locus_tag="PKH60_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16651.1"
                     /translation="MINRVLLKSIFLLIFLLCVSFVGTTTILAANPVDSTIGTIDTPP
                     GVDKQIAASNLATDEIAIFFFLSSLIKIANVIAGLWVAFNIVFAAFNYLSGQGSADAH
                     KKVRDKITMSFVGLFLLAIAYMAVAVISLLLFGDAGYVLKPDIVSPL"
     gene            complement(6484..7074)
                     /locus_tag="PKH60_00955"
     CDS             complement(6484..7074)
                     /locus_tag="PKH60_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16652.1"
                     /translation="MLELFEATVKTQINLFKKTLLIISSILFGFALSSSQAQAACSFI
                     RDSGGTVFCDYNTNGSIDLGEMTFATCTANPNACCTTVAECSTSPTADCNPGSGFIPL
                     GDCLKLSDDTKVSETYTTPAFLVNLLVRNLFVVAGIILFFMILLAGFKFIAGGKKGLD
                     DAKQIMTAALIGFLLMFSAYWIVQIVKLITRTNIVL"
     gene            complement(7058..7753)
                     /locus_tag="PKH60_00960"
     CDS             complement(7058..7753)
                     /locus_tag="PKH60_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16653.1"
                     /translation="MRTISKIITTFFASFFFLMIISPLDASASCGTIKNDPPNWGTIK
                     FCDYNNNNQGEDPIEFSFVSCTTGQANDGPLGASCCSDAAGCGVVPSVTPTSGPPAPT
                     TPAGSPTPSGPIANPLEPPTDAFFDLVDPLQHGGGQTVLDDVPSAYASQLRSPGGIVS
                     RLLVFAFPLAGLILFMMLVWAGFEMLVGAPTKKSMDAGKQRATAALVGFLLLFATYWI
                     FQIIEVVFGVRII"
     gene            complement(7753..8988)
                     /locus_tag="PKH60_00965"
     CDS             complement(7753..8988)
                     /locus_tag="PKH60_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16654.1"
                     /translation="MKGKTTLVGILVFLGIFLGVVPTKALALEDCSTRGITIVSSNLA
                     GPFSFSDPVQLNIRVGSSGTPGLITGNSYRLFIRDGQLTRESPSQVVGASGQLEWLNF
                     NSFASPGEHTLQLAGGGLSADGCRLGTYTVTVGGIDCAYLRVSKDWNAAPSNGNECYA
                     TDTSCVENGLFGSNLLVVAKLTKSDGSPYTRPVIFSLHGPNNAGNDEGETNNGPPSYE
                     YSYNGFSPDHDGTYTITVKLNEAGLNNPPICQGTFLQADNCQSCITTPNNIAEQINDP
                     FALEHHAYKICDQVSGSLMTPEGANAKDRCIACIGGDELGRAGIWTAVGCIPREPEKI
                     VNSLLRLGLGMGGGFALIIILASGFVLSTSQGEPKRIEDAKGWLTSALAGMLFIIFSV
                     TLLHFIGYSIFKIPGFGGP"
     gene            9982..10857
                     /locus_tag="PKH60_00970"
     CDS             9982..10857
                     /locus_tag="PKH60_00970"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNQ16655.1"
                     /translation="MQNIDVCFKYDANKKCTDLRSFSFEAEYLQGIYEMPGSWHMEAL
                     KAQAIAARTFAIKYTGNGSKSICTTEACQVFKNQKKGGDWERAVNDTRGWVLVDSGGN
                     PVSTQYASTHGGYSNTSGWDTTDKSADGSWSTRAWENKANSPWFYKAWYRNGYSSSGD
                     SCGRSHPWLSQEEFSDIINAWIVRSNPNGADTSRIQPPTINQCQIGGGGNPYSMNELR
                     DKANNSGGAVTNVSSVSVSHSSNGQTTTVNVNTNRGNLSIPGSEFKSTFNLRAPGYLR
                     IPQSSFGFFNIEFKQ"
     gene            10903..12009
                     /gene="recF"
                     /locus_tag="PKH60_00975"
     CDS             10903..12009
                     /gene="recF"
                     /locus_tag="PKH60_00975"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNQ16656.1"
                     /translation="MLQSLHLQYFRSHEDLKIDFFDGCTLIVGPNASGKTSIIEAIHL
                     LATGQSFRAGKVEEMVGFSAEWTRITGNLSALSGDITDEDAGEEPEQTELSVLLTRGM
                     LQGKKTHHKHFSVNGINRQLHKMLGQLQTAVFRPEDMRIIEGSPSRRRRFLDEVIGSF
                     DAEYLRSAKTYEQSLKRYNKLLETVRDNRQPTTILEYWERSMVKHGEYLQRQREQFLL
                     ATKQVSFAVRLQVEYKPSIITQERMDHYQERAVAAGHSLIGPHKDDFSVSCDVQDLWR
                     GQLSQFHNVALYGSRGQQRLAVLWLKACQLNYIENQTGQKPLLLLDDIFSELDEENQQ
                     VVVKLINNHQAIVTSADQLVASFLPKNTAKLQLE"
     gene            12387..12797
                     /locus_tag="PKH60_00980"
     CDS             12387..12797
                     /locus_tag="PKH60_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNQ16657.1"
                     /translation="MTDHYILDASICLALLFNDNPLQNIKAKRFFRQVESSIFKADLS
                     LLTLNDLIISIEKMYDLNRRQYMMELRDLVSLKGIRIIEIKKFDCLHLLRKYEKNHLT
                     FTDTYLLWLSEKQKLTLMTVNRRLENYCSSSSKL"
     gene            12815..13639
                     /locus_tag="PKH60_00985"
     CDS             12815..13639
                     /locus_tag="PKH60_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNQ16658.1"
                     /translation="MEITSEVQSFLKQIKLPSPDSHKGQNGKLLVIGGTELFHSSIFW
                     AADVASKIVDMVHFASPANENNQVVRQKIKAGFWTGIVVDWSDVDYYLAEDDAVLIGP
                     GMDRSEETEKIVNQLLSEYPTKKWVIDGGALQMIDVKLLSKSHIITPHHKELDLVLEK
                     IDSLNRELAMTQLLETGVTLLAKGTEDKVMQNSTSVEITGGNAGMTKGGTGDVLAGLV
                     AALYCQHDALTAAVVASLVNKQAGEVLWETVGPYFSAGDLLNEVPKSFWRLLQSNS"
     gene            13682..14773
                     /locus_tag="PKH60_00990"
     CDS             13682..14773
                     /locus_tag="PKH60_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNQ16659.1"
                     /translation="MFDPVFSITNNLLTYITRIEAAKAIIDHSPLIPAWEARFRSEAL
                     NRTVHYGTKIEGNELTEEQADQLLKLQFVNDSKEALRQTGITARERDVQEVINYRNVI
                     TWTDRQTQLKTTQVFSLDTLNTLHRLTVQGLIDDEAVGHFRKKQVIVQSATSGGVVFR
                     PPVAVEVPYLIEDFFIWGKSESAQQIHSILRAAITHYQIVYIHPFIEGNGRTARAMAT
                     LLLYTLGYDFKRFFSLEQYFDTDVEAYYQALLSVQQTGKQDLTYWLEYFCYGVALEID
                     KIQRQVERLSKDLRMKKQLGKQVALSERQIILLELFQQQDQISSYDAQNTLPNVSVDT
                     ILRDLKDLIEKGVIEKEGVTKGVTYRLRE"
     gene            14811..15044
                     /locus_tag="PKH60_00995"
     CDS             14811..15044
                     /locus_tag="PKH60_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNQ16660.1"
                     /translation="MQSITTVTQKGQVTLPIGIRQLLKIKSYDKVMISFSRGKIIIEP
                     VLNILDFADSVSVNPKVSVSQARKLMEKNYRPV"
     gene            15041..15469
                     /locus_tag="PKH60_01000"
     CDS             15041..15469
                     /locus_tag="PKH60_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNQ16661.1"
                     /translation="MKHATEYCLDTNVFLRVLIRENEQAYQSSKKFLESIQRGQIKAY
                     IHELVMAEMIWTLQSVYDQSKEKTIVLAESILNLNGLEMISGFDQRNAFQLYQYYSVK
                     YVDACLASIIQVKQKKWTIVSYDGDFKKLPVLWLKPEQII"
     gene            15515..17305
                     /locus_tag="PKH60_01005"
     CDS             15515..17305
                     /locus_tag="PKH60_01005"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HNQ16662.1"
                     /translation="MKFAEFAQHLKALEETPSRLAMTEQLVALFNKLEIVEIQPACYL
                     LQGRLTPQYESLEFNLSIKMLLRALNRLVSEVGYAERFYKQLGDVGLVAEKIILDRLA
                     QIGSVVSQKELDLIQTFDTLVLLAKESGEGSQERKLDRLVELFSSSDGLSARYIARIV
                     IGKLRLGFSTMTILDSLSWMADGNKHWTPDLEKTWEKRADLGCLATVWIAECRQPKPN
                     YPDVLESYTVRVGTPVMPALCQRLNSAQEIIEKLGEVIVEPKYDGLRLQIHLYIDDQT
                     HQPTVKAYSRSLEDCTHMFPELLTITDQINCQSCILDCEAIGFDPLTGKLKVFQETIT
                     RKRKHEVETHSQSVPIRFYVFDVIALNGNSLLAQPLIERKHILRDLFSVTGPTEQPDS
                     RGCFYITPYIETKDASEVHQFHETALADGLEGAVIKQRDSQYQSGRKGWRWVKIKETE
                     GSQGKLSDTLDVVMMGYYKGRGKRTQFGLGAILTGVIDSFGKIVTVGKIGTGMSEDQL
                     RELKQLGDEHLSVTKPEEYGEVDKSLQPDIWLEPSVVIEVAADELTESPVHSAGYAMR
                     FPRLLKIRDDKTWEQATTLAEIQQMRKLSA"
     gene            complement(17322..18419)
                     /locus_tag="PKH60_01010"
     CDS             complement(17322..18419)
                     /locus_tag="PKH60_01010"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ16663.1"
                     /translation="MSIKRETPILIVSSTEGHLSMANSIESGLKSQGFTNVTTYVENS
                     AILPYRLYYRYAPSLHKLSSLLLGSMSLVSTQRKILRLRYNKLAKQLVEKHRPEIVIS
                     TNYALNSSFEALMIKNPLNYLTVICDPRSYNLVNVTEGKGINLVFDAKQVQIISRDFP
                     SAICKETGWFVRPEFQPPTSNLEAKRAVGVDSSKFCALLVAGSEGMLRIQNTVRALME
                     KQPNLTIVVACGKNQLLYQTMKFMAKQLENSQMQILPLSFTSAIAPYFQAADIIMGKA
                     GPNMIFEAVACHKPFFATTSLGGQETGNLDMIKELDIGFVETDPEKAAKVLVNLANDS
                     SKIAKLEPKIKKLAEYNSQSGKRLVDLLDVK"
     gene            18443..18730
                     /locus_tag="PKH60_01015"
     CDS             18443..18730
                     /locus_tag="PKH60_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16664.1"
                     /translation="MKKVEINQPWHLLIVATLLFCSFGAVLHIGLLLLQALILGQPFP
                     LGLISILGLQFFFPSLKSGIFESLLSFIAYTFCIALLYRFLKSLHITIEKK"
     gene            complement(18733..19608)
                     /locus_tag="PKH60_01020"
     CDS             complement(18733..19608)
                     /locus_tag="PKH60_01020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ16665.1"
                     /translation="MKTKTTSVDFSIIIPALNEEKYLPLLLKDLAEQSFSKKNFEVIV
                     VDGNSKDQTIKVATNFSKSIQLTVIPSKTANVSIQRNLGVKQSNAKWVIFMDADNRIP
                     PAFLDGIKYQLATNQRIDCFTCWIDESGYKTADKPLITMYNLLIEVLSKVKPGALGAL
                     IGTRRALAIKHPFNDELAMSEDYEFIGTLVKSKYHFVVFRFPRYHFSLRRFEREGVLK
                     LARVYTKAQLYVLMGRQIKNGEIDYPMGGDVRIAKSPWHQSSLKLLQSNITDLLKNHK
                     STAQKILNWLSFEDN"
     gene            19655..20194
                     /gene="pth"
                     /locus_tag="PKH60_01025"
     CDS             19655..20194
                     /gene="pth"
                     /locus_tag="PKH60_01025"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNQ16666.1"
                     /translation="MKVVIGLGNPGKMYQHTRHNVGAKLVEDLAEGAVWKENKAASAK
                     LALNSDNQVLFVLPTTYMNRSGVSLTYVKKKYPKIGNEDLFVIHDDLDIKLGEYKIQF
                     GKGPHEHNGLLSLYQVWGSSAFWHIRIGIDGRMGSRDVPGQQYVLQPFSPDEVVKLEN
                     VALSLIPQVKNQLQQEYTQ"
     gene            20191..21414
                     /locus_tag="PKH60_01030"
     CDS             20191..21414
                     /locus_tag="PKH60_01030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ16667.1"
                     /translation="MRSFAYLASIPKKYFFASRDLKLLYYERVIRDLVNKLAFFFFPI
                     YLFQLGTNLLPSSLALTQWQAGLLTLAGYFILSKLLVVMLVIPIGKLTSKIGYQRALA
                     YSYIFRLVSFLSLFLSVSFPLAIFGSVVMEAFQVAFYWPSYHTLLANNSLKKNLGSNL
                     GGLQVLLLLIGAVTPAISGILNTQLGFQFVFFLGILGAMASFCLILLISDSCDLDTVS
                     FSEFKEWLGEKRYERLTTAIAGRYVYDVVLFIWPFYLFTLFGSVERVGILATLSLFLA
                     LIVSIISTFKLDSLKSRTPYKISGVTIALTWFLRTQLFSVWGVALADALDRVSSNYHW
                     LFFDTIWYKRGKGSQAHSFFVYHELLQNLAVCIIWMVILAVLLISGNWKILFILSGLG
                     VLATLMLKEKGSDKN"
     gene            21401..21706
                     /locus_tag="PKH60_01035"
     CDS             21401..21706
                     /locus_tag="PKH60_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16668.1"
                     /translation="MTKTKSANEKQTSFSSIGSLFDDYKVEDKGGYITQEFQDFGYRL
                     ACELNDEKHKSLYIKMAKTLPRHLLEEARRFVFDAQSAKSKAKLFMWKLKQLRSGKL"
     gene            21709..22902
                     /locus_tag="PKH60_01040"
     CDS             21709..22902
                     /locus_tag="PKH60_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNQ16669.1"
                     /translation="MKFPKTTLLLILLLLFFCVVLGYNYVWRPFAGSMVATENQEDQA
                     TTAGELTAVQPSRRWQLTNLTPEQKVGQLLMVPFDVNLSTASDEAKLQQWLTLEPGGV
                     VLFGKSISAIEAKRFSTLLTQSSAPDSISTILAVDHEGGDVQRLAGNGFSILPSAREL
                     CKQTEEIRVAELSQSAQELSLAGIHMIFGPVVDVSSSSGVLKSRVCSGDPALVGQYGK
                     EFVAAFSNQGILPVVKHFPGIGSITRDLHTSFDQQLVVAKDVLPFKSILDAFPTIGVM
                     VSHMGIVNQIPDLPCSLSADCIDQLKSTNPQALVISDSLTMKAASFDGETNTYTRTLE
                     SAALSAMYAGNQVLVFGPETTPAELQKVKSALVAQYAVDPSFAKQVDSAVERILDLKE
                     FQIFK"
     gene            22925..23125
                     /locus_tag="PKH60_01045"
     CDS             22925..23125
                     /locus_tag="PKH60_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16670.1"
                     /translation="MSKISLWKTLSSKKIYENDWISLRHDEFQTPNGKKGIYGVVGGK
                     DFSLVIPKMGDDYFLLPKNVFE"
     gene            complement(23092..>23641)
                     /locus_tag="PKH60_01050"
     CDS             complement(23092..>23641)
                     /locus_tag="PKH60_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16671.1"
                     /translation="LSNFHTYLSIPESIVPNSRTSKESMKIVRVDNLTYYVTLFGTGF
                     FLLSTAITLINAIYFESFFLICLSGAFLHNFLRAKKYRIELSKGMIREITQTKTSEIS
                     FSTITQLDCKEIDSGDSRNFHAIFLLQTENGPVEFETNLQASLFDIALWVRDNYPSAI
                     FTKKLNKYLAAPHSKTFFGSKK"
BASE COUNT         7068 a         4673 c         5160 g         6740 t
ORIGIN      
        1 ccgccaacta atcctgcgga gagactgggt cctttttgca aaatatccca accaaaggtt
       61 cgctcgcaag gaaggttgta tccaatcaaa acgtcttgac agccagttgg tagaccagta
      121 tcggggtcgg tgccactaca aacctgttga taaacttttt ggcaactatc aatatcgtag
      181 acacctccgc cttgaccaac ggggatcata tccggctgtc cattccagtt gggtggttca
      241 attcgatcat cagaaaaagt atgatctctg gttccaccag taaattcaat ggaatcacca
      301 cttagttgga tgcgatcaaa ttcctcagct gaggtggtta ggctttcaat ttgatcggtg
      361 gtaaagaagg tgcctgctaa cacctcttgg actgtttcca tatcgtagcc aactggatac
      421 acaaggaaat gtttgacctc gcgctcatca caacagccgt tgtagggaaa gcttgggtaa
      481 gtccggtcgg tgacatgaaa cagagatttg agtccccagt cgcgaggaga gccgagtggg
      541 ttttcgccat cgtctggatc acctggtttc caagtttcgg caaaagcggg attgctttct
      601 cccttgggtt ccacttgatg agtggcatct aattcacccg ttggatcctg atttttatct
      661 ggtacttcaa aaatattatg cagtaattca gcgccaaact cttgggtata gagtaactgt
      721 gcgggtggat ttttaacgaa ttcttctgtt ccaaatccac cgaaatcaag aacgctcgta
      781 gctggttctt taaccaattc tggacattcg ggatcaattt tattattgta ctttgttgta
      841 actcgtgagc tggtttgggc attcacaata tcaataactg cctttcctag ctcgtctttg
      901 cattctgggg ggccaatacc agaagttgaa gcgccaacta agcaaaatat ctcatcactg
      961 ctcgtttgcg cgtgggtgtc ataccagttt gcagcagttt gcagttcggc tttttttgca
     1021 cttgctgtcg ccacgtattt atcttgaatt ttcctggtga tgagcgaatt acgagtcaat
     1081 ttacttgaat ccatccaagg agtattacca ctactagaca aaccaacaac ctcttcttga
     1141 gtagtggtaa gtatgtctgg caccttgaag gcattgacca aaattgcgtc gtctggtttg
     1201 gatggtccac caacgggacc ctcattggga tgaatgtaga agttaaacat actggcatag
     1261 gttgggtgtt ttaattcaat cgttgtaact aggaatgcga gtcgataagc ccgatcaatt
     1321 gttagtggag taaacagaaa accttgtcga gcagtttcgt tccaatctgt gctgacaaca
     1381 gtatcacaca ccgcctcgat agtaattgga tccgtgtatg cggggagggg tacgggttgt
     1441 atatctggat ggggtacaga ggccagagct ttgtactcat caagcattgt tttaatagtg
     1501 taattagtct gaggaaccgg tctggagtat aaagcgcatg cagaaggatt taccaactta
     1561 ttacacatct cttcttgttt aagtaaaata cggacggaag cctcacatct ttgttgattg
     1621 gtgagcaggg aattgatggc tgctgaattg agctcagctt tagtattttc ttgaatgacc
     1681 gtatccttaa agccaaaaaa ttcttctagt gaagtagtga ggaagcgttt atctgaaacg
     1741 tcacgaaaaa ctggagtttg aacctcacga taatctaccg caagccatga gcggacgtaa
     1801 ttaccgatac caggaggacc actggttctg gttcgtatgt aataattttc gtaaccgcca
     1861 aagacagctc cgggcggaaa ctgttctaca taatctgcca tccagtcttt accatcttga
     1921 gtcaattcaa ttttaaaacc tggtgtggca caacgagctt cgtaaccttg atcaatcagg
     1981 tcgtcacgaa tggtttttaa atttaagtct acacccccag aacttccgag tgatctgttg
     2041 aggcctttga ttggatgaat atagaactga tcttgctcag cattgtaagc atcgtaaatt
     2101 gagatatcat atcctgcata caattcagaa attgatgtcc agggaccagt ttctgtgatt
     2161 accactggtc tgcccaactc gccgaccaca ccggccacat ggttggtcca gatgttttgc
     2221 atggtatcgt tgacaggata aatattgcca gcgacggcat agagagcgcc aaaatttgcg
     2281 ccagcgcgtc tcatggctct gggaatgcca tctggtctgc ccgtatcgtc gttaaacata
     2341 aagctggtca ggttgaaagc gggtgagagt agtcgcacat tgctgggcag tcccgcgcca
     2401 actagggtat tcatatacgt agctagtttt gggccgacgc aatcgtagaa agctgctttt
     2461 tctgcaggtg ggctggagcc atttggcgcg ttacacgttg gcgcagccca gtgctcaata
     2521 tctggttcat tggggcctgc gatggcataa aatggtctgc tagtaagccc agctacggct
     2581 cgcaaaaagt tggcatagtc ggcaggatta tcaaaacctt cgcttccctg agtggttcca
     2641 atccgaataa tgacattatc aacttgttcg ttgttaatga catctgcaac cgtctgaggg
     2701 cttgtagatg ctctggcaat ttctaaagtc cacttcattt taaattgacg agctttttcg
     2761 tagtttcctc ccttgtaata cgtggcatag ttaactccaa gcccagctgc gccacagtca
     2821 ccatcacata gttcattcag ttgaaaatca gtgagttggt gcttttggaa gtttggatct
     2881 ggattagtgc caaaaacgtt aaataagaga gccgactcaa ccggggtttg actcattaaa
     2941 aactcaaagt ctgcttttaa gtcggttaaa tcatacgcac tggcaaaatt tgggtgtaac
     3001 aaaacagctg aaaataacac aaacacaacc aacgtgatta ggcgtaggcg aaacatcaga
     3061 tctattatat agcctttcgg ctagcaaggg acagagagtc taccaattaa tagcagatgt
     3121 tggaatacgg aaggttttaa tcacggtcac tccggtgaag tcagcgatta aattgatgat
     3181 aatgaaggcg ctcagtgcca agactaaacc gagtatggca taggtgaacg tgcgtttggc
     3241 cgaatcaatg ttttttgagt tggatccagc ggtgagccac ttgaatgctc ctaagatgaa
     3301 catgacaaaa ccacccaggc caatcaaggt gatgatcacg gtgaaaatgt ttgcgattaa
     3361 acactcaagg ccttgaatgg tgggcacatc gccagatgaa ccagccaagc acacaccaga
     3421 ccatgttctt gtttgagctt gggcttgagc gggaatagcg aagctaaggc ttactaatgc
     3481 gacaaatagt gaaagagcga caagatgatt tctggctggt ttcatgatac aggttagtat
     3541 actacaaaac agcccggcga atgccgggct gtttaaattc aataacaata gagcatcgcg
     3601 tttagcgagt gttaatttgt ctaatgccac cgaatgcatc ttcaagatta tcaaatccaa
     3661 gaatctgcag tagtagcatc aggatagcgt aagcagcggc taatacaatc aaaccgatga
     3721 tagcagcggt aattttgtta cgggctgatt cggttttgcc tttgtctcca ccagaggtaa
     3781 tccattcaaa ggctccccag ataagataca aaaacacagc aagtgcggct aacgccatca
     3841 cgatactgag taaactgctg ataacaccac caaggtttgt agcgaatcct ttagctggaa
     3901 ctgagacggt accaatactg acagcagcaa aggcacttga ggctgttaaa aataaagctg
     3961 ttccagcggt actggcaccg gcaacaatct ttgtcaaggt ttttttcata tctatccctt
     4021 cgataagtac tgttactact ataacgcgtt ttttactaat ttgtaaatac gcctgtgctt
     4081 cgtattatac cagaagagcg attttgaagc aaagttcatg ataaatattt tcagtcgctt
     4141 gctaaggagt aggcgtgggt acgggaaggg ctgcaggtgg cgccacacag gctgtagccc
     4201 agaaatctaa tacgcaaata ccaagcatct gttgaactaa cataaacaac gctatcactg
     4261 agctaagcac aatgatacca atgactgctg tggacatccg attgcgagct tcatcgagct
     4321 tgcctttatc tccagaagaa gtaatccaag caaatcctcc ccagattaaa tagataagca
     4381 acattataaa tgcgatggaa atcacaatgc taagaataga tccgattaaa tctgcaaatg
     4441 cttgtttgaa cacattggat gaagcgctag ctccaggaat atctgctgtg ggcaaatctt
     4501 ggttacgggc ttcctcagtg atatcgaaat caatcgtatt ggctgccagt gcagaaaaag
     4561 gccgagcaac aaacaaagac acaacaagta gtcctgttgt aatggtggat agtatgcggt
     4621 ttgattgcag cattgtttta ttccggtgta aagaatttaa aattgagtac ttggaaggta
     4681 tcaccaaaga gtaggttgcc cacaaaacca atgatcacaa acgaagaaac gaggatgagc
     4741 aggccgatgg cggcttgcaa catgcggttg cgagccgctt ctaacttagt tttatcacca
     4801 ctggcggtga tccattggag ggcaccccag acaaaaaaga gcagtaacag tagtccgccg
     4861 atgctaataa tattattcca atagcgaact atttgagaaa gaaacaatga gccatcggcg
     4921 gctttatctg ggtcattacc caagttccct acggctggat tagtgatagc ggcttgagcg
     4981 gtttcaatta ttttcatggt actatggttt ctaatacttc tgctgggtta agaatattca
     5041 ttccaacgat actgccaatt agcccaacca gcgcatacat catggttaag acaattaatc
     5101 ctaatgcgcc ttgcagcatt tggtctctgg ctttgccaac tttagcagtg tctccaccag
     5161 aagtaatcca ggaaatagcg ccgagcatga agtaggtaat gaatactaat cctcccagta
     5221 cggttaacag ggccaggata ttagaaacga ttttttctag agtagtaaag gctgttgagc
     5281 caacttcgag atctttgaca tactcgttat caggatcgaa tggtgccaca atgccttctc
     5341 caaattgcgc taaaacccgg accggcgaag ctagaacaac aagtatcccc agcaggtaga
     5401 aaactgattt gagtctgttt cgcataaccg tactactatt atacataact ttcttgatta
     5461 gcctaaggtg cagttgcacc atactgacta atatctggtt ttaagatgaa tcccgcattg
     5521 ccaaaaaaga ctaatccaat aattgctgcc aacatgtagg cggcaacaat tattactagg
     5581 ccaatcatgg cgtactgcag tcgctcacgt acatcggtga aggctttggt atctcccgcc
     5641 tttgtgatga gagcgtagcc cgcccacaag aagttgaaaa acataaatag tccacaaatt
     5701 acagtaaaaa gacgtagggc aacggaaata aatgagaaaa ttccaatgtt ggtgcagttg
     5761 ccagtatcac aattataaac accaactcct tgaggtgcgg agataccgcc aataatagca
     5821 gacccgcaat cagccccatt gggggcaact gtatttggac caattaagat gcaaacctta
     5881 ttatttggtc cgcattgtcc gatggaagtg catccattta aacaattgtg atttggcggg
     5941 gatatacagg tgtctgcatt cacgggggag attgaaccga aaaataagaa aaagaagctg
     6001 ataaaccatg gaagaataaa tagaaaagtt aatcgtttca tagtgggcta acaatatcag
     6061 gctttaatac atacccagca tcaccaaata atagcagact aatcacggcc acagccatgt
     6121 aggcgattgc aagcaaaaat agacctacaa aactcatggt gattttgtct ctaacttttt
     6181 tgtgagcgtc tgcggatcct tggccactga gataattaaa ggctgcaaac acaatattga
     6241 atgccaccca aaggccagct attacattgg caattttgat cagtgaactt aaaaagaaaa
     6301 aaatggcaat ttcatctgta gctaaattgg aggctgcgat ttgtttatca acacccggtg
     6361 gtgtgtcaat tgtgccaatt gttgagtcaa ccggattggc tgccagaatt gttgttgtgc
     6421 ctacaaacga cacacaaagc aagaagatta gtaggaaaat ggattttaat aacactcgat
     6481 ttatcataag acgatattcg ttctcgtaat gagtttaaca atttgtacga tccaataggc
     6541 agagaacatg agtaagaaac caataagtgc ggctgtcatt atttgtttgg cgtcatcgag
     6601 tcccttttta ccaccagcga tgaatttgaa gccggctaac aaaatcataa aaaataagat
     6661 aatgccggct acgacaaaca agtttcgcac caaaagattg actaagaaag cgggtgtagt
     6721 ataggtttcg ctcactttgg tatcatcaga tagtttcaaa caatcgccca atgggatgaa
     6781 gccagagcca ggattgcagt cagctgtagg tgaggtggaa cattcagcaa cagtggtgca
     6841 acaagcattg ggatttgcag tgcatgtggc aaaagtcatt tctccaagat cgatagagcc
     6901 gttggtattg tagtcacaga acacagttcc acccgaatct ctaatgaaac tgcatgctgc
     6961 ttgtgcctga gatgaagaaa gagcaaaacc aaataaaata ctacttatta tgagaagagt
     7021 ttttttgaat agattaatct gtgttttcac tgttgcctca aataattcta acaccaaata
     7081 ctacttcgat aatctggaat atccagtagg tggcaaacaa aagtaagaat ccaaccagtg
     7141 ccgcagtcgc tcgttgtttg ccagcatcca tggatttctt agttggggca cctacaagca
     7201 tttcaaaacc cgcccagacc aacatcataa ataggattaa gccagccaag gggaaggcaa
     7261 aaacgagtag ccgactcact attccacccg gactgcgtaa ttgactagcg taagcagaag
     7321 gaacatcatc aagtaccgtt tgtccgccac catgttgtag agggtcgact aaatcaaaaa
     7381 aagcatcagt tggtggttct aatgggttgg caatgggtcc actgggagtt ggggagccag
     7441 cgggagttgt gggtgccggt ggtcctgagg ttggtgtgac gcttgggaca actccacatc
     7501 cagcagcatc gctgcaacag ctagccccaa gtggtccatc gttggcttgt ccagttgtac
     7561 aacttacaaa agaaaactca attgggtctt caccctggtt attattattg taatcacaaa
     7621 acttaatagt gccccagttt ggagggtcat tttttatagt cccgcaggat gcggaagcat
     7681 ccaaggggga aataatcata aggaagaaga agctagcgaa gaacgtggta ataattttac
     7741 taatagttct cattatggtc ctccaaatcc tggtattttg aagatactat agccaataaa
     7801 gtggagcagt gtcactgaga aaataatgaa cagcatgcct gctaaagcgg aggtcagcca
     7861 acctttggca tcctcgatgc gcttgggctc tccctgagaa gtgctgagaa cgaaacctga
     7921 tgcaagaata ataattagcg cgaagccacc gcccatgccg aggcctaggc gaagtaagct
     7981 attgacaatt ttttctggtt ctcgcggaat acagcccacg gccgtccaga tgcccgctcg
     8041 acccaactca tcaccaccaa tacaagcaat acatcgatct ttagcgttag cgccctctgg
     8101 tgtcattaat gacccactga cttgatcgca aattttatat gcatggtgtt ctagagcaaa
     8161 cgggtcatta atctgttcag caatattatt tggtgtagta atacaacttt gacagttatc
     8221 agcctgtaga aaggtacctt gacaaatagg tgggttattt agacctgcct catttagttt
     8281 tacagttatg gtatatgtac catcgtggtc tggactaaaa ccattgtaac tatattcata
     8341 actaggtgga ccattgtttg tctctccttc atcatttccg gcattatttg gaccatggag
     8401 tgagaaaatg actggtctag tgtatgggct accgtctgat tttgttagct ttgctactac
     8461 gagcaaattg gatccaaata gtccattttc cacacaactt gtgtcagttg cataacactc
     8521 attgccatta ctgggcgcgg cattccagtc tttacttact ctaagataag cgcagtctat
     8581 gcctccgacg gttactgtat acgttcccaa tcgacatcca tcagcgctta atccaccacc
     8641 cgctaattgg agggtgtgtt ctccggggga ggcaaaagaa ttaaaattaa gccattctaa
     8701 ttgtccactt gcgcccacca cttgagaagg agattctcgt gtaagttggc catctctgat
     8761 aaacaatcga tatgaattac ctgtaattaa tcctggtgta ccgctggatc caactctaat
     8821 attcagctgt actggatcac taaatgaaaa tggaccagca agattgcttg aaacaatcgt
     8881 aatacctcta gtactacaat cctcaagggc caacgctttg gttggcacca cgccaagaaa
     8941 aattcccaag aacaccaaaa taccaaccag agtggtttta cctttcataa cttacgtgtt
     9001 attgtacctt aatacactct gagttttaag aggggtaatt attgactcaa gattgatcaa
     9061 actagtatga aacgattcgt taaattagta ttgaaaatag gaactggaat ttttttacca
     9121 ttgttaattt ttggagcact ggtccttcaa cccggttttg ttcgcgcact agaccccgtg
     9181 gatgacctgc aaaatcaaat caacgaactg gagaagttaa aaaatttgtc ggaagcagcg
     9241 accaaaccgt tggaaaaaga agtggccaac ttgcaagcgc ggatcaactc tgccagagcc
     9301 ggtattgaaa aagccaagca agaggtggtg caagttggca acgatatcga aaaaagagaa
     9361 gtagacctag ctctccaata ccaaatttta tccaagcgtg tgcacgatca atatatacga
     9421 ttacgtttag ttgcctcgcc cttgcttatg ttatctcaaa atacggtggg aaacttaacc
     9481 aaggatttag cctatcgtga atcagtgcag gcacaagacc agcgattaat tagtagtatc
     9541 actaaagaca tagccgaact agaaagtgat cgtcaagcac tagaagagcg ccaagttgtt
     9601 ttggccaact tagaaaaaca gttggatagc caagctgctt tctttaaaac agaaattgcc
     9661 aaagccaaag actatcaaca aactctctca agtaaaattg ctgatctctc cgcgcgacaa
     9721 caatcaatta ttaatgcacg atcgggttct tttattacct ccgtgggtga agtaccactt
     9781 gccgatgact ttaacgcttc tattggcttt aaggctcagg ctccaagcaa ttcttttgcg
     9841 gtttttagtt ttggtggtta cacgcatcgg aatgggatga gtcagtatgg ggcgaaagcc
     9901 agagcagaag ctggtcaatc tgcagaggag attttaaaag cttattatcc caatgctaca
     9961 ttaaaaaaag actattctac gatgcaaaat attgatgttt gtttcaagta tgatgcaaat
    10021 aaaaaatgta cagatctcag atcttttagc tttgaagctg aatatttaca aggtatatat
    10081 gaaatgccag gaagttggca catggaggca ctcaaggccc aagctatagc cgcccgcact
    10141 tttgcgatta aatatacagg taatggttca aaatcaatct gtactaccga agcttgtcag
    10201 gtgtttaaaa atcaaaagaa gggtggagat tgggagagag ctgttaatga tacccgaggt
    10261 tgggtgttgg ttgatagcgg aggtaaccca gtttctactc aatatgcctc aacgcatggt
    10321 gggtatagca atacatctgg gtgggatacg accgataagt ccgctgatgg atcttggtct
    10381 acgcgagcat gggaaaataa agccaatagt ccctggtttt ataaagcttg gtatcgcaat
    10441 gggtattcgt cgagtggcga tagctgcggc cgctcgcacc cttggctctc tcaagaagaa
    10501 ttttcagaca ttattaatgc ttggattgtg cggagtaacc ctaacggagc agatacgagc
    10561 aggattcaac cgccaactat caatcaatgt caaattggtg gcggtggcaa tccgtattcc
    10621 atgaacgaat tacgtgataa agctaataac tcaggtggag ctgtgacaaa tgtatcatca
    10681 gttagcgtca gtcatagtag caacggtcaa accaccacgg ttaacgttaa tactaatcga
    10741 ggtaatctga gcattcctgg tagcgagttc aaatcaacct ttaatttgcg tgccccaggt
    10801 tatttgcgaa tcccccaaag tagttttggc tttttcaata tcgaatttaa acagtaggtt
    10861 aatcagatga tcctgcctgt atactactgg cagcttggga ttatgctgca atcactacac
    10921 ttacaatact ttcgttctca tgaagatctt aagattgatt tttttgatgg atgcacgttg
    10981 attgttggtc ccaatgccag tggcaaaaca tcaattattg aagcaattca tttactagcc
    11041 actggccaaa gtttccgtgc cggaaaggta gaggaaatgg tgggtttttc tgccgaatgg
    11101 acgagaatta cgggcaatct gagcgcttta tccggagata taacagacga agatgctggc
    11161 gaagagccag agcaaactga attatctgtg ctgctcaccc gcggtatgct tcaaggcaaa
    11221 aaaacccacc acaagcattt ttcggttaat ggcattaatc gacagctcca taaaatgctc
    11281 ggtcaactgc aaactgcggt gtttcgaccg gaggacatga gaattattga aggttctccc
    11341 agtcgacggc gacgattttt agacgaagtg ataggtagtt ttgatgctga atatttgcga
    11401 tccgccaaaa cctacgagca aagtttgaaa cggtataaca agttgctaga aacggtgcgt
    11461 gataatcgcc aaccaacaac gatactagaa tactgggagc gctcaatggt caaacatggc
    11521 gagtatctgc aacgtcaacg tgagcaattt ctattggcca ctaagcaggt gtcttttgcc
    11581 gtacgtttgc aagtggaata taaaccatcg attatcacgc aagaaagaat ggaccactat
    11641 caagaacgag ctgtggctgc tgggcattca cttattggtc cacacaagga tgatttttca
    11701 gtcagttgcg atgtgcagga tttgtggcga gggcaactaa gtcaatttca caatgttgct
    11761 ttatatggat cgcgtggcca gcagcggctg gccgttttat ggctcaaggc ttgccaatta
    11821 aactatattg aaaatcaaac tggtcaaaaa cccctgcttt tacttgatga tattttttct
    11881 gaactagatg aggaaaatca gcaagtggtg gtcaaactca tcaacaatca tcaggcgatt
    11941 gtgacttctg ccgatcagtt ggttgcatcg tttttaccaa aaaatacagc gaaactgcaa
    12001 cttgagtgat tactttaatt actcataact tttcaaacca ttacagttaa agcaagaatg
    12061 tgcgtgtagc ttatatcaaa ccagtatttg caagcatagt gggtaaagta aatattgaaa
    12121 agtaaagtag tttgaaaaaa gtaaagttgg cctatggtag ttgaaaacga tctgtccaat
    12181 accgcttgta gtcagaaaca cactgcgacc atttcttcga aacttcaaat tactttacct
    12241 aagcacatgc tgaactctct tggattgaag ccgaaaagaa aggtttcgat tgagttgcat
    12301 cagacgatca tgatcttgaa accagccgta gtttcatcgg atcagctttc ggggtgtcta
    12361 agtaaaaagc tattgtctaa ttcataatga cagaccacta cattcttgat gcatcgattt
    12421 gtctagcatt actttttaac gataatccac tgcagaatat caaggcgaaa cgttttttta
    12481 ggcaagtcga atcgtcaatc ttcaaggcgg acctgagtct tttaacactc aatgatctta
    12541 ttatttcaat tgaaaaaatg tatgacttaa atcgaagaca atacatgatg gaactgcgag
    12601 acctggtgtc acttaagggc attcgaatta tagagattaa aaagtttgat tgcttacatt
    12661 tattaagaaa gtatgaaaaa aatcatttaa cttttactga cacttactta ctttggttgt
    12721 cagaaaaaca aaaacttacg ctcatgacag taaatcgaag gttggaaaat tactgttctt
    12781 caagctcaaa actgtaggta caatatgcgt agctatggaa atcacttcag aagttcaatc
    12841 atttttaaaa cagatcaagt tgccttcgcc cgactctcac aaaggccaga acggtaaact
    12901 tttagttatc ggtggcactg agttatttca tagcagtatc ttttgggcgg cggatgtggc
    12961 gagcaaaatt gtggatatgg ttcattttgc gagcccggct aatgaaaaca atcaagtagt
    13021 tcggcaaaaa attaaagctg gcttttggac tggtattgtg gttgattggt cagatgtgga
    13081 ttactatctt gctgaagatg atgcagtgtt gattggtccc ggcatggatc gctcagaaga
    13141 aactgaaaaa atagtcaacc agctactttc agaataccca acaaaaaagt gggtgattga
    13201 tggaggcgca ttgcaaatga ttgatgtaaa actactgtcc aaatcacaca tcattacacc
    13261 gcaccacaaa gaacttgatt tggtgctaga gaaaatcgac agtttaaaca gagagttagc
    13321 aatgactcaa ttacttgaaa ctggagttac gttgcttgca aaaggcacag aggataaggt
    13381 gatgcagaac tcaacttctg ttgagataac tggcggtaat gcagggatga caaaaggtgg
    13441 cactggcgat gttttagctg gattggttgc cgcactgtat tgtcagcatg atgcactgac
    13501 ggctgctgtg gttgcgagtt tagtgaataa acaggcaggc gaggtcttat gggaaacggt
    13561 aggaccatac ttttctgccg gcgatctttt gaacgaagtg cccaaatctt tttggcgact
    13621 tttgcaatca aattcctaaa attccctgca aaaattgcgt gaattgcggt atactcgcat
    13681 cgtgttcgat ccagtttttt ctattactaa taatttgcta acgtacatca ctcgcatcga
    13741 ggccgcaaaa gctattattg atcactcacc actgattccc gcttgggaag ctcggtttcg
    13801 gagtgaagcg ttgaatcgaa ccgttcatta tggaacaaaa atcgagggca acgaattaac
    13861 agaagagcag gctgatcagc tactcaaact tcagtttgtt aacgatagta aagaagcctt
    13921 gcgtcaaacc ggcattacag ccagagaacg tgacgtccag gaagtaatca attatcgtaa
    13981 tgtcattact tggacagaca gacaaaccca actcaaaacc actcaggttt tctcgcttga
    14041 cacactcaat acccttcacc gcttaacagt tcagggactt atcgatgacg aagcggttgg
    14101 acattttcgt aaaaaacaag tgattgtaca aagtgcgact agcggagggg tggtatttcg
    14161 tccgcctgtg gcggtggaag taccgtattt aattgaagac ttttttattt ggggtaagag
    14221 cgaaagtgct cagcaaatcc actcgatttt gcgcgccgcc attacccatt atcaaattgt
    14281 ctatattcac ccttttattg aaggcaatgg acggacggcg cgcgccatgg caactctctt
    14341 gttgtatacc ttgggctatg acttcaaacg attcttttcg ctggaacaat attttgatac
    14401 cgatgttgaa gcatactatc aagccttact ttccgttcaa caaactggca aacaggattt
    14461 aacgtattgg ctggaatatt tttgttatgg cgtagcgtta gaaatagata aaattcagcg
    14521 ccaggtagaa cggctttcca aagacttacg aatgaaaaaa cagctaggta aacaagtggc
    14581 gctttccgaa cgacaaataa ttctgctcga actatttcag cagcaagatc aaatttcttc
    14641 atatgatgcg caaaatacct tacccaatgt ttctgttgat acaattttgc gtgacttaaa
    14701 ggatttaatc gaaaagggcg tgattgaaaa agaaggcgta accaaaggtg ttacgtacag
    14761 gcttagagag taattgacaa agtaatactt tatagtatag taatacatat atgcaaagta
    14821 ttactactgt aacacaaaaa ggtcaagtca cactacctat tggcattcgc caattattga
    14881 aaattaaatc atatgacaaa gtgatgatta gttttagtcg ggggaaaatt ataattgaac
    14941 cagtgctaaa cattcttgat tttgctgatt cggtttctgt caaccctaag gtttcagtat
    15001 cacaagcaag aaaactgatg gaaaaaaact acagacctgt atgaaacatg caacagaata
    15061 ctgcctagat accaatgttt tcttaagagt attaattaga gaaaatgagc aagcttatca
    15121 gtcctcaaag aagtttttgg aatcaattca acgtggtcaa ataaaagcgt acatacacga
    15181 gttagtgatg gcagaaatga tttggacttt acaatcggtc tacgaccaat caaaagaaaa
    15241 gacaatcgta ttggcagaaa gcatattaaa tcttaacgga cttgaaatga tttcgggatt
    15301 tgatcaacgt aatgccttcc aactttatca atattattcg gtgaaatatg tagatgcttg
    15361 tttggcctca atcattcagg tgaaacaaaa aaaatggacg attgtttcat atgatggtga
    15421 tttcaaaaaa ttacctgtat tgtggttgaa gcctgaacaa atcatttaat tatcactgtc
    15481 ataaggtcgt cttagatgcg ttactatata tagtatgaag tttgcagaat ttgctcagca
    15541 cttaaaagca ttggaagaaa caccttcgcg cctagcaatg acggaacagt tggtagcctt
    15601 atttaacaag ctagaaattg tagaaattca accagcttgt tatctactgc agggtagatt
    15661 gacgcctcaa tatgagtctt tagagtttaa tctgagtata aaaatgttgt tgcgtgcgtt
    15721 gaataggttg gtgagtgagg ttggctacgc agaacgattc tataaacagc tgggcgatgt
    15781 gggtttggtg gccgaaaaaa taattctaga tagattggca caaataggct cagttgtttc
    15841 acaaaaagaa ctagatttaa ttcaaacttt tgacacacta gtcttactag caaaagagag
    15901 cggggagggt tcacaagaaa gaaagcttga tcgattggtt gagttgtttt caagcagtga
    15961 tggtttgagc gcgcgatata tcgcgcgcat cgtcattggc aaacttcgac ttgggttttc
    16021 caccatgacg attttggatt cattaagttg gatggctgac gggaataaac attggactcc
    16081 agatcttgag aaaacatggg aaaaacgcgc tgaccttggt tgtttggcta cagtgtggat
    16141 tgccgaatgt cgtcaaccaa aacccaacta tccagatgta ctagaatcat atacggttag
    16201 agtgggaacg ccagtcatgc cggcgctctg tcaacgcctc aattctgctc aggaaatcat
    16261 agaaaagtta ggagaagtca ttgttgaacc aaaatacgat gggcttcgct tacagattca
    16321 tctatatata gacgatcaaa cccatcaacc aacggttaaa gcctactcgc gaagtttgga
    16381 agattgtacc cacatgtttc cagaactgtt aactattaca gatcaaatca actgtcagtc
    16441 atgtatttta gattgtgaag ccattggctt tgatccacta acaggcaagt taaaggtttt
    16501 tcaagaaacc atcacgcgaa agcgaaagca tgaggtagag acacattctc aatccgtacc
    16561 aatcagattt tatgtttttg acgtaattgc gctcaatgga aatagtctac tagcgcaacc
    16621 gcttattgaa agaaaacaca ttttacgcga tttgttctct gtaactggtc caactgagca
    16681 gccagatagt cgaggttgtt tttatattac gccctacatt gagaccaaag atgcaagcga
    16741 ggtgcatcaa tttcacgaaa cagccttggc agatggtctt gaaggagcag taattaaaca
    16801 aagagatagc cagtatcaat ctgggcgtaa gggttggcgg tgggtaaaaa ttaaagaaac
    16861 cgagggatca caagggaaac tatcagatac tttggacgtg gtaatgatgg ggtattacaa
    16921 aggcaggggc aagcgaaccc aatttggttt gggagcaatt cttactggtg taattgatag
    16981 ttttggaaaa atcgtcacag ttggcaaaat tggtaccggt atgagcgaag atcaattgcg
    17041 cgaactgaag cagttgggtg atgagcactt gtcagtgacc aaaccagagg aatacggcga
    17101 agtggataaa tcgctccagc cagatatctg gctggagccc tcagtcgtga tcgaggtcgc
    17161 cgccgacgaa ctgactgaat caccagtcca tagtgctggt tatgccatgc gatttcctcg
    17221 cttgctcaaa attcgcgatg acaaaacttg ggaacaagct acgactttag ccgaaattca
    17281 gcagatgaga aaactatctg cataattctc tgagcaaaca tttatttaac atctaataaa
    17341 tcaaccaatc tttttcccga ttgagagtta tattctgcca atttctttat ttttggctca
    17401 agttttgcaa ttttagatga gtcgttggca agattgacta atacttttgc tgctttttca
    17461 gggtcggttt ccacaaaacc aatgtcaagt tctttgatca tatcaagatt acccgtttct
    17521 tgacctccca gtgaggtagt agcaaaaaag ggtttatgac aagcaaccgc ctcaaaaatc
    17581 atattgggac ctgcttttcc cattatgatg tccgcagctt gaaagtaggg agcgattgca
    17641 gaagtaaatg aaagaggcaa aatctgcatc tgtgagtttt ccaattgttt tgccataaat
    17701 ttcatggttt ggtatagcaa ttgattttta ccgcaagcta cgacaatagt cagatttggt
    17761 tgcttctcca tcagtgccct cacagtattt tggatacgaa gcatgccttc cgacccagcc
    17821 actaataaag cacaaaactt ggacgaatct actccaactg ccctttttgc ttctaagtta
    17881 gacgtcggcg gttgaaactc tggtcgaaca aaccacccag tttctttgca tattgctgag
    17941 ggaaaatcac gactaataat ttgtacttgt tttgcatcaa acaccaagtt gatgcctttt
    18001 ccctcagtca cattgactaa gttataacta cgcggatcac aaatcaccgt caaatagttg
    18061 agcggatttt taatcatgag cgcttcaaaa cttgagttga gggcatagtt agttgaaata
    18121 actatttctg gacgatgttt ctcaactagt tgtttagcta gtttgttata tcttagtctt
    18181 agaatttttc tttgagtgga aaccaaactc atcgatccaa gcaacagact cgacaactta
    18241 tggagcgatg gtgcatatcg ataatagagc cgatagggta gtatcgctga gttttcaaca
    18301 taagtagtaa cattcgtaaa accttggctt tttaatcccg attcaataga attcgccata
    18361 cttaaatgtc cctcagttga ggaaacaatc aagattggtg tctcacgttt aattgacatg
    18421 ggtgtattgt atactttgcc gtatgaaaaa agtcgaaatt aatcaacctt ggcacttact
    18481 aatagttgcc actttactat tttgtagctt tggtgcagtt ttgcacattg gtctattact
    18541 gttacaagca ttaattttgg gtcagccatt tcctctcggt ttaatttcta ttttgggatt
    18601 acaatttttc tttccatcat taaaaagtgg tatttttgag tcattactgt catttattgc
    18661 ttacacattt tgtattgctt tactctatcg atttttaaaa tcactacaca ttactataga
    18721 aaaaaaataa gtttagttgt cctcaaaaga aagccaattg agaatttttt gagctgtgct
    18781 tttgtgattc ttgagcaaat cggtaatatt gctctgcagt agcttgagag aagattggtg
    18841 ccaaggtgat tttgctatcc gaacatctcc acccataggg taatcgattt caccattttt
    18901 tatttgacga cccatcagta cgtagagttg cgccttggta tacactcgtg cgagcttaag
    18961 tacgccctct ctctcaaatc ttcttaagct gaaatgatat ctgggaaaac gaaatacaac
    19021 aaagtggtat tttgacttga ctagagtgcc gataaactca taatcttcag acatagcaag
    19081 ctcatcattg aagggatgtt taatcgccaa ggcgcgcctt gtaccaatta atgctcccag
    19141 agcaccgggt ttaacttttg aaagcacttc aatcaataag ttatacatag taatgagtgg
    19201 tttatcggct gttttgtaac cagattcatc aatccagcag gtaaaacaat caatcctttg
    19261 attggttgct aattggtatt taattccatc cagaaacgca ggcgggatgc gattatctgc
    19321 atccataaaa atgacccact ttgcattaga ttgttttacc ccgaggtttc gttgaataga
    19381 tacattggca gttttggatg gaatgacggt gagctgtatg cttttggaaa aattagttgc
    19441 taccttaatt gtttgatcct ttgaattgcc gtcaaccact atcacctcga agtttttttt
    19501 agaaaatgac tgctcggcaa ggtccttaag caacagaggt agatattttt cttcgttcaa
    19561 agcggggatg ataattgaaa aatcaactga agttgttttt gttttcacga acgctattat
    19621 atatggttac ccaacgtgtt atgatttcag gcacatgaaa gtcgtaatcg gattgggaaa
    19681 tccaggcaaa atgtatcagc acacgcgtca taacgttggt gcgaagttag ttgaggattt
    19741 agctgaaggc gcagtgtgga aagagaataa agctgcaagt gccaagttgg ctttgaattc
    19801 cgacaatcaa gtactatttg tgttgcccac aacctatatg aatcgttcgg gagtatcgct
    19861 gacttacgtc aagaaaaaat atcccaaaat tggcaatgaa gatttatttg tgattcatga
    19921 cgatctagat attaaactag gagaatacaa aatccagttt ggtaaaggac cacatgaaca
    19981 caatggtttg ttgtcactgt accaagtctg gggttcctca gctttttggc acattaggat
    20041 tggtattgat ggaagaatgg ggtcgagaga cgtaccaggt caacagtatg tgctccaacc
    20101 atttagccca gatgaggttg tgaaactcga aaatgtcgcg ttatcactga tcccgcaagt
    20161 taagaatcag ttacagcagg aatatacaca atgagaagct ttgcatattt agcaagtatc
    20221 ccaaaaaaat atttttttgc ctcaagagat ctcaagctat tgtattacga aagagtaata
    20281 agagacctgg taaacaaatt ggcttttttc tttttcccaa tttatttatt tcagttgggc
    20341 acaaatttgc ttccgagttc ccttgcgctc acacaatggc aggcaggttt gcttactcta
    20401 gctggatatt tcattttgag caaactattg gttgtgatgc tagttatccc aattgggaaa
    20461 ttaacgagca aaattggtta tcagagagcg cttgcttact catacatttt tagactggtt
    20521 tcgtttttga gtttattttt atcggtttcg ttcccattgg caatttttgg aagcgttgta
    20581 atggaagcat ttcaggtagc attttactgg ccgtcttatc atacgctcct ggcaaataat
    20641 tcactgaaaa aaaacctggg ctcaaacctt ggtggcctac aggttttatt gctattaatt
    20701 ggagctgtta ctcctgcaat ttctggcata ctcaacaccc aacttggttt tcaatttgta
    20761 tttttcctcg gaatcttggg agctatggcg agtttttgtt taattttact tatttctgac
    20821 agttgcgatc tagacacagt tagtttctca gaattcaaag agtggctggg agaaaaacgc
    20881 tatgaacgac tcactacagc catagctggc agatatgtct acgatgtcgt gttatttatt
    20941 tggccatttt acttatttac cttatttggt tcagtcgaaa gagtggggat attagctacc
    21001 ttgtccttat ttttggctct catcgtgtcg attatttcaa cgtttaaact tgacagtcta
    21061 aaaagtcgga cgccatataa aatctccgga gttactatcg ctctcacttg gtttttacga
    21121 acgcagcttt tttctgtttg gggggtagct ttagcagatg ctttggatag agtgagcagt
    21181 aactatcatt ggttgttttt tgacacgatt tggtataaac gtggcaaagg atcacaagca
    21241 cattcttttt ttgtctatca tgaattatta cagaatctag cagtgtgcat tatttggatg
    21301 gtgattttag cagtacttct gatcagcggt aactggaaaa tactatttat tctctctggt
    21361 ttgggagtgt tggctacact aatgctcaag gaaaagggaa gtgacaaaaa ctaaatcagc
    21421 gaacgagaaa caaacttcat tttcttcaat tggatcacta tttgatgatt acaaagtaga
    21481 agataagggt gggtacatta ctcaagaatt tcaggatttt ggctatcgtt tagcctgcga
    21541 attaaatgat gaaaaacata aaagtctata tataaaaatg gctaaaactt tgccccgtca
    21601 cttgctagag gaagcccgca gatttgtgtt tgatgctcaa tcagcaaaga gcaaagcaaa
    21661 gttgtttatg tggaagttaa aacaacttcg atctggtaaa ctatagttat gaagttccca
    21721 aaaactactt tattgctgat acttctgctg ttattttttt gtgtggtact tggatataac
    21781 tatgtgtggc gaccatttgc tggatcaatg gtcgcaactg agaatcagga ggatcaagcc
    21841 actaccgcag gtgagttaac tgccgttcaa ccttctcgtc ggtggcaatt gaccaatctg
    21901 actccagaac aaaaagtggg tcaattgctg atggtacctt ttgatgtaaa cctaagtact
    21961 gcctcggatg aagccaaact gcaacaatgg ctcactctgg aaccaggtgg tgttgttttg
    22021 tttggcaagt caattagcgc tattgaggcc aaacgctttt ccaccttgtt aactcagtca
    22081 agtgcaccag atagtatttc tactattctc gcagtcgatc atgagggtgg agatgttcag
    22141 cgattagctg gtaatggatt ttctatttta ccgagtgccc gcgaactttg taaacagact
    22201 gaagaaattc gagtggcaga acttagccag tcggcacagg aactgtctct cgcgggcatt
    22261 cacatgatat ttggtccagt ggtagatgta tccagcagca gtggagtgct taagagtcgt
    22321 gtgtgcagtg gagaccctgc gctagttggt cagtatggca aagaatttgt tgctgcattt
    22381 tctaatcaag gtattttgcc ggtagtaaaa cattttccag gaattggttc aatcactaga
    22441 gatttgcata ctagttttga tcaacagctg gtagtagcga aagatgttct gccattcaaa
    22501 tcaattttgg atgcttttcc tactataggg gtaatggttt ctcacatggg catagtcaac
    22561 caaattccag atctaccatg ttcgttgagc gctgattgca tcgaccaatt gaaaagtacc
    22621 aatcctcagg ctctagtcat cagtgattca ctcaccatga aggccgcgtc cttcgatggt
    22681 gaaactaata cttacacgcg aacactagag tccgcggcgc tctcagcgat gtatgccggc
    22741 aatcaagtac ttgtatttgg tcccgaaaca actccggcag agttacaaaa agtcaaatcc
    22801 gcgcttgttg ctcagtatgc tgttgatccg tcatttgcta agcaggttga cagcgccgtt
    22861 gaacgaatcc ttgatctcaa agaatttcag atttttaaat aatagatgat gcaaattaca
    22921 taaaatgtca aaaatttctc tctggaaaac gcttagttca aagaaaattt acgaaaatga
    22981 ttggatttct cttcgtcatg atgaatttca aactccaaat gggaaaaaag gaatttatgg
    23041 agttgtaggc ggtaaagatt tctcgttggt gattccaaaa atgggtgatg actacttttt
    23101 acttccaaag aacgtctttg agtgaggagc agcgaggtac ttattgagtt tttttgtgaa
    23161 tattgcagat ggataattat cccgaaccca cagagcaata tcaaataatg aagcttgcaa
    23221 gttggtttca aattcaactg gtccattttc tgtttgtaac aaaaagattg catgaaaatt
    23281 tctagaatca cctgaatcaa tttctttgca atcaagttga gtaatagtag agaagcttat
    23341 ttcggaagtt tttgtttgag taatttctcg tatcatccct ttagaaagtt cgattcgata
    23401 tttcttagct cttagaaagt tgtggagaaa cgctccggat aaacagataa ggaaaaaact
    23461 ttcaaaatat atagcattta tcaaggtgat tgctgtagaa agtaaaaaaa agcctgtacc
    23521 aaatagtgtc acataatacg tgaggttatc gaccctaact attttcatac tctctttgct
    23581 agttcttgaa ttaggcacga tactttccgg aatggaaagg taagtgtgaa agttagacaa
    23641 a
//
LOCUS       DAOQZP010000009        23216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_2556_length_23216_cov_5.783515, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000009 DAOQZP010000000
VERSION     DAOQZP010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 23216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23216
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2556_length_23216_cov_5.783515"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PKH60_01055"
     CDS             <1..858
                     /locus_tag="PKH60_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNQ16672.1"
                     /translation="DEFSMTKSSEGVLLCQRYGDKIEVDSFSTLTGGGGGNTAVGFAR
                     AGFHTAVVTETGKDVLSNLIVDSFHQEKVATNLIIQEKKEQTGGSVILIGQDGGRTVM
                     VHRGAASLLDPVDIPVDSIEKTGWVHLSSIAGRQATLETIFKARKNRKMSWNPGKQEL
                     YLLAESQLAFNALPIEVLLVNEQEWAMVDLVQPALTSHIPTIVVTNGNQGGRIISKGQ
                     ETHFSSQSIPSVDDTGAGDAFAVGFVCGYIWQQPLDECAVIGAKNAASVVGHIGAKPG
                     LLRKGDLLH"
     gene            874..1932
                     /locus_tag="PKH60_01060"
     CDS             874..1932
                     /locus_tag="PKH60_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ16673.1"
                     /translation="MSDFRQILDQEIASLKAKIDTAVMPEEMRLKVQKDIVALERSVE
                     LGSYDEKYEKVSKYIDWILQIPWEKESEDQLDVAKTKEVFEKHHFGMMDVKDRFVEYV
                     SVLKLRQQQFADSDFRAPVLLLVGLVGTGKTTFAYSLAEALGRTIVRIPFGGMGSAKE
                     LRGESRLLLGSEPGRIIKGICEAGVRNPVILLDEIDRSAEEANRDIMGVLVELLDPAQ
                     NHAFRDTYVDYPVNLSHAIFLATANNTGNIATAVMDRLEKISMPSYSDEEKTVIAKKY
                     VLPQLLESAGLKPEQLIIEDAVWPAVVRPLGYDMGIRTLQRTLQGAVRKAARIIVEKG
                     YSEVVINEQNRKIFLPQY"
     gene            complement(1938..2672)
                     /locus_tag="PKH60_01065"
     CDS             complement(1938..2672)
                     /locus_tag="PKH60_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16674.1"
                     /translation="MSLPFTEQERAVAELADQETNSKIQVLLENKLTLLEKADSYLDL
                     VSEFVLKISEDSISTEISQIFPRGDKESYHHPKQLLFDGFHKLEDTLIDPKDIEVARN
                     RVQELREQLTNLQATFSRAENGYFSPGLIVKDPDGTLHLVLDAVDYEGGNSQYLFLLS
                     VHNHFRDNLDQTRFVVEDDPSSYSYFLTQIASKWFEFNIYALEGIWEEKRETYLDSDP
                     RVRTKPHFAPKPFEFALAIKSLLEVR"
     gene            2779..3414
                     /locus_tag="PKH60_01070"
     CDS             2779..3414
                     /locus_tag="PKH60_01070"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNQ16675.1"
                     /translation="MFEKNRSASLSQIIFDLEKSVDQLPIAEKPTDIVFGEGSPDSEI
                     VLIGEAPGYNEHVQRRPFVGRSGQLLRKTLTQVGFPPEQVYITNIVKARPPDNRDPSP
                     EEILAYKPFLDQEISLIDPKLIITLGRFSMAKFLPDVKISQVHGRLHKVRWNGQIMYV
                     LPMYHPAAALRSTQMLTAFTQDFEKVAKILIWVKEQSELIVDLETISAILL"
     gene            3436..4257
                     /locus_tag="PKH60_01075"
     CDS             3436..4257
                     /locus_tag="PKH60_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNQ16676.1"
                     /translation="MSPEELKERNFYQEIKPVLDQLDAELLRLREQPLAIEHKEGVGS
                     QAALVTAADRFADEYLRKELKKLFPEFGFVTEEGEHQATEEYVWIIDPIDGTNNYAHG
                     LDMFGVSIGLWRQDGTSVLGVIDLPARKTRIWGGKGQGVWHDGKRLSTPSQLAPEKAM
                     LTIAPIWSSPAQGGAVTQALAEKVGHTRDHGSNVWQSFLVLIGQVNANLSFSLSIWDI
                     AAAVALAGELGLSVVFSNNVQRHNLIQQADQPVTVAIGQQTLAEQLVAAVEPWIS"
     gene            4343..5425
                     /locus_tag="PKH60_01080"
     CDS             4343..5425
                     /locus_tag="PKH60_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16677.1"
                     /translation="MKNILFGFVCFVLVSLFVRPIFAQGTIGTANPVIWKTAYNEFSS
                     SNFYIQIGERTFYGSDAIRVNSDPGIDKTTLELEWKENDITMRFYLYFEKIENGMWQL
                     YDMRTYNATGTDWIYYQPVDSLGNSVSSLIGQRNYAYERTFLSQDGSAKIYCKECSIT
                     AFISNIAPPSVHGYVIDFRIGIPQNETITITNEPNTGYGVNAVLMDASGSVVKDQSDF
                     TYSWKVENTSTLSIYAQGVPYPDGNCAYGILAPCPDFNVQIRGINPGISRVLLDITRK
                     SDQTVVASNAFTVRVIEKTLSPTPSPSPTVKITPTPFQDDVTAQLTELQGEVGRLTNV
                     VEKQQVEITVLQRIIQTIQEFLKNLF"
     gene            5526..6002
                     /locus_tag="PKH60_01085"
     CDS             5526..6002
                     /locus_tag="PKH60_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16678.1"
                     /translation="MSKQEKLIQISNWFLFLHIIYKLGVFVGCFVLLWMFSYMIGLNE
                     STSESFSLVGAIIVTTVLLLNNILYWKNSGVYVTKQGDHIVKQGGWFVKEDKLLNGII
                     IANQVTRNPIDQLLGMASIQTGLFGDKHLTGVRYRDIQSYDDTMRSSMNGTFSSMF"
     gene            complement(6042..>6250)
                     /locus_tag="PKH60_01090"
     CDS             complement(6042..>6250)
                     /locus_tag="PKH60_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16679.1"
                     /translation="AHFSMLYDGSDSTTTTLTTEQKQKAAEQLRQAVQLPLTYETSGI
                     AIHTSTPVVTEWQQVAFVPFGNED"
     assembly_gap    6251..6350
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6351..6916
                     /locus_tag="PKH60_01095"
     CDS             <6351..6916
                     /locus_tag="PKH60_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16680.1"
                     /translation="YGITAMKALTKSLFLKRHKDPLADLFQQTEYIHLWFSHLVMFIG
                     VAAVLTVLAAINLFIPFQVVNSTIITIVMIALGVVFGICSYIILLLSNPKQEGYRLLK
                     IVKLVYGVFFSFYIFSYFLISPPLATSHSFYWFCLFIFATFVVCSFSTYKSPRANSFL
                     ERVTDVFRDYSWGNNITLIKPNKQRKA"
     gene            6916..8577
                     /locus_tag="PKH60_01100"
     CDS             6916..8577
                     /locus_tag="PKH60_01100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNQ16681.1"
                     /translation="MSELQLLPLGGMGTVTQNMFVYEYENELLLVDCGIGFPDMQMPG
                     VDILIPDVAYILTKLEAGKRIVGMILTHGHDDHIAALPYLLPHLPHFPIYGSPLTVGF
                     ARQRVSEITLEPEFHVVAEREEFRIGSHFSARGYRITHSVPDTRHYAIKTPAGLIYHG
                     SDFKLDEHPVDGLTSDLEHIGQLKQEGVLLMMLDCLRVEYDRPVKSETTVGPVISRLM
                     KETKGKFVVTLMSSHIHRIQQVIEAAAEEGRRVAFIGRSVEQNVRIAQELGQLQYNPS
                     MVVDKRDLDQVPDSKACLIVAGSQGQEGSSLVRAIFGEHPVFQIKPTDTVVFSADAIP
                     GNELAYFAAIDQLASNGVHVLYPDVEADLHESGHASRVEQKQMVDLVRPEFVMPIGGA
                     DRHRFKFKEFVAEPAGYDAKHTLLPVSGEILGISASGIRVVDKITLKPPVVDGLGVGD
                     VGPAVLSDRRNLSQAGMVVVVIPKIHGKLAVEKTEVISRGFVFMKEAEEVIDFIRDVV
                     RETVQQNQKTNEGDLKRAIEKRLARKLYKIIRREPLILPVILPIS"
     gene            8632..10818
                     /locus_tag="PKH60_01105"
     CDS             8632..10818
                     /locus_tag="PKH60_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNQ16682.1"
                     /translation="MKKRSKRRHPKLKMKPETTQAIVGVGLITFGLLILISFSGQGTA
                     LIAINKFLVEKLGASLLLLPFLLIAAGLSLFRTKFRWTQPHVLLGSLILFIGFVLLFR
                     TGTIGLNSFESTSRLISKAGTIIASISLLLIGILVMTQLKVQEMIDVFGLLKRKPTKS
                     TEKDTVDEKQPGRFHLPSVHLPALPFRSKEKTPDERFVDDDGAKKTTKPPLLEPLITP
                     PPKETPPTESLPKSTNIAPSTTIWHLPPIDLLEEAKGVKANRGDVSKNADTIEQTLES
                     FGIRAEVKEVNRGPAVTQYALEISRGTKLSKITGLSTDLALALAAPTGQIRIEAPIAG
                     RSLVGIEVPNYSAEFVNLRDMLEKPIMKKQANKKLLVALGTDVTNQPVAVDINAMPHI
                     LIAGATGSGKSVALNSFLCSILFRATPQEVRLILVDPKRVELTWYKEIPHLLTPVIVE
                     SKQVVASLNWAVHEMENRYKLLHEVGVREIAGYNEVAGHSAMYHIVIVIDELADVMMY
                     APAEVEEAVTRIAQMARAVGIHLVLATQRPSVDVLTGLIKANIPARIAFNVSSMTDSR
                     VILDTPGAEKLLGKGDMLFISPDRAKPIRVQGTYVSDKEIKRLTQYLRDQDFEPQYLE
                     EVTSKYKVMPGKSGVLDTNGDEKDELFLEVCKILITQDKASSSSIQRRFSIGYNRAAR
                     ILDQLYAAGMIGPSDGSKPRGINATRIMEYIHHEEGTNGSSSENNE"
     gene            10865..11785
                     /locus_tag="PKH60_01110"
     CDS             10865..11785
                     /locus_tag="PKH60_01110"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNQ16683.1"
                     /translation="MKKQYLWAIVVILVLAGATLFVTRSQDLRMPVTMPEFVNTQSTS
                     NDSFAIGAEPAGYMEKGVMVSDVYQPMPPYIGGGDALTVEERQYQTYASFSVVVKDVS
                     GYLNQLREKYLGMEGRVLNLSTNTSGKYQNGYLTAKVPVASFDSASQEATKGVEKIVD
                     QSVNSTDVTGVAVSFEEQIQQLETQRSQREVDLLEAKTDAEKKRIQLDINRLQNQIES
                     LKKQKVNQQTQVQYATITVSAASSEKVFNPSTRPDLGETLREAVDSVLNNLFEVAQFF
                     IWAAVYAVVLLPALWLIGKLRKPKTTVTTE"
     gene            11869..12816
                     /locus_tag="PKH60_01115"
     CDS             11869..12816
                     /locus_tag="PKH60_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ16684.1"
                     /translation="MKNILVVNQLKKIYPPATKRESEFCALDGISFTVKKGEIVGLLG
                     PNGAGKSTTIQMLIDTLKPTSGTIEYFGKDLHQNRSEVLQHVTFASTYIKMPWRLTVM
                     ENLWIYALLYGLNREQFNQRVESFLTFFGVWDQRHKTIGMLSAGQITRIMLAKAFIPH
                     PKIVLLDEPTASLDPDMAHQVRDFVKRQQKEYGMSIIYTSHNMDEVAAVCDRVIFLNH
                     GKIVALDTPDNLAKSVSLARVRLWVKDGLKRTERFANEQGLVSHIEDREIVIEIDEPA
                     IAGFLASLADAQIEYSQISIDKPTLEDYFLSMSKQEQTK"
     gene            12813..13601
                     /locus_tag="PKH60_01120"
     CDS             12813..13601
                     /locus_tag="PKH60_01120"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ16685.1"
                     /translation="MNIAKHRLQALFTRHLYPLKRDFDLLSDMIYWPLIDTVLWGVTG
                     MWLTETSGVPELVVALLMGLIMWNIIWRSQSEVGRNMMDEIWNSNLVNLFSTPVSILE
                     WLISVIGLSFVKTLVTLTVIIPVMVLLYSINVMALGLWLPVFFVLASMTGWSVGLLAS
                     GIVLRWGPRVQTVIWTLPGILLPFSAIYFPAERLPGVLQTISWFVPTTYVMETLRAVV
                     FNGSPEIMMLVISFGLNIFYLVLASIFFLRSFEYSKNMGLNRFT"
     gene            13642..14703
                     /locus_tag="PKH60_01125"
     CDS             13642..14703
                     /locus_tag="PKH60_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16686.1"
                     /translation="MRRFLTYFQRVSGGYLLALICLMVLSGVVTYVVCQYQNQTIRLR
                     NQFLEQRYKEQMKNEVDARQPTRQPVKDSVETERIYESRDGKLLTKIYWSPNDYGLDV
                     ETIDLDTKVVKRHSENDYFKLIKRVEGLFSNSLESVFLLTRDSKQYAVIDTYVGNVDV
                     GDGLREIYVATFEPIKESLTIKSFSITPVSAKTISISRAYGRVEILDYFPQQNQIITI
                     SRSSDGCGGMGSVELLSVSIQNQVVKEVGMGCVTQTRYLGYLGNLLYFGEIDLTDEDL
                     ENWEDAKIINIFSLNPLTKERKNLSIDLSTHAFDTTWVDVTNKISATELVLFDKTTEE
                     GYALDVKTLELRNLGKLYD"
     gene            14755..15777
                     /locus_tag="PKH60_01130"
     CDS             14755..15777
                     /locus_tag="PKH60_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16687.1"
                     /translation="MAEVPTIKEKSIVTPLPEVVIPESVRAQFALAIKRIMPLFMDWN
                     VHDECWVDSSVKPDLIITLARAGSLAWVPMAAIADLYDIRLPKTFDLHLGRELAYRFI
                     RESQGDFRLLQNGDAKTIADFKKWLDKEFNLNPDLAEAIANISESLAANDKSKNIVVF
                     DDVVQDCITMGIIIPRLIERSLATNVTFVSSNEKLQKRLAIRDLSSEIGQTTKVTLEC
                     ILDDTEWLYGIIDNSFSEYFDSLYLEKKIVVRQLLVQLAKGSIEDIQTGALNLITNEA
                     QIRKIGENIQQQYLTNNAPRHEAMFIDPGNPADEILETYGVTALLTLQIAVREELERL
                     GQTVEL"
     gene            complement(15801..16916)
                     /locus_tag="PKH60_01135"
     CDS             complement(15801..16916)
                     /locus_tag="PKH60_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16688.1"
                     /translation="MKKQNPVVCHPGWWGYWSEIVETFPHLAARVNVHETILIQGNSA
                     LNGFINIGDPVCIPSDPDQAWFEENGLTALFDQPRSFFLVSNLALIALDGPLPIGDAI
                     VVGRVAREGVSLARIVKTTAPIVGVTVLGWLGLRNIPPNTPIIVSSQVHPMNTALGRY
                     NQFTATKMSSFSLAGRYFVLPWSGGAIPEDSVEVIWIERPQSSLVSRGFVLVPTWREG
                     VIFPQGLAPEYLHDYAIVRGNGHLDDTNSGIGRPEMRRADASQIATIGMTNPIQCWKK
                     RPEPGRWGSARALVYQTASGLVTFLHNLDNNDRTFLRGNIPEDDLEEFDRDWRMVTAT
                     HWEPCNPIERNLILMILDEVLDYLGIPHSPVEINKPE"
     gene            16933..17112
                     /locus_tag="PKH60_01140"
     CDS             16933..17112
                     /locus_tag="PKH60_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16689.1"
                     /translation="MHPPAGQGRGVGHGVAVGVRVGDAVGTAVRVGVRVGVAVATVVR
                     VGVGVLVGQGVDVAG"
     gene            18189..18914
                     /locus_tag="PKH60_01145"
     CDS             18189..18914
                     /locus_tag="PKH60_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16690.1"
                     /translation="MKKMFFTILLLMLLPISVFAGGGRILIYTSDNLGGYYYVNSDMY
                     VLAQVFVPDSSNTIASIGERAEFRIENPRAGDSCVNEIEQTTELGLLRARCRVTQPGM
                     LTVYVYSFADNYESSRYILNFYAAPSPTTIPTQLPTVIPTTVKSTLTPTIAVNNSITT
                     APVELTPTPTSSSDSNALLKDYVFEESVATVSSEVDLDTNSENRIAQSTESRTIVLSL
                     ALLMFGICIAGVGLIIFKTSKKT"
     gene            19185..19508
                     /locus_tag="PKH60_01150"
     CDS             19185..19508
                     /locus_tag="PKH60_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16691.1"
                     /translation="MEFLRKIVNRNKRESKDVDYPNEFTLEQCISELTGDGFTVSSAL
                     RDDVARFGLVEGIVLAMDGNSGLRVVNWNGEGLVIKTFNGLRDGKDLLSKLARVKYDS
                     KPIHL"
     gene            complement(19537..19980)
                     /gene="ssrA"
                     /locus_tag="PKH60_01155"
     tmRNA           complement(19537..19980)
                     /gene="ssrA"
                     /locus_tag="PKH60_01155"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(20024..20677)
                     /locus_tag="PKH60_01160"
     CDS             complement(20024..20677)
                     /locus_tag="PKH60_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNQ16692.1"
                     /translation="MLFIKLLSKIVFGILAPTYCVSCERWGELLCDKCYETIDFLYEQ
                     PLPQLAPLYLDDVISACRYGGAIKALLKSLKYQSVIDAGRLCGRLLYHCTIIPPVDCI
                     TSVPIHPMRLRERGFNQAQVIAQTLAELVNTPYQDLLIRTIHGSHQASIQSRDERLDH
                     VVNHFSLNPKLTSAIPKTILIIDDVLTTGSTLNECARIVKVNGCKTVIGLTVARQAS"
     gene            complement(20739..22406)
                     /locus_tag="PKH60_01165"
     CDS             complement(20739..22406)
                     /locus_tag="PKH60_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ16693.1"
                     /translation="MRIRHQLILLFCLTALCYLPFVNSPFIWDDQQFVTENQTIQSFN
                     FAQMFKESTTAGGGINSNYYRPLTSISFAIDTGWWGNNPIGFHLTNLVLHLTAGLLLF
                     WLLINLGLSNRWRFWVTTFFLLHPVQTEAVTYISSRGDSLSTVFLLFGLNSFWLSFQK
                     KKLHFSLYDLQISFSQPFLLFTSALSLILAVLSKELALSGLGLYGLILLLHLFQTQQT
                     IKVIFSKYLPTTIAIVFLLILGLGYTYVRQTTLNFDPDFDYSVIDPQYPSSLSVRLLT
                     FTRALPRYIGTFAFPYALHMDHVLPVIHSILNPWTISVFLVSILIVLLSILEKNKLKT
                     LWLGFGVLWFVIGLVPISGIVPVNGLFYEHWLYIPIVGLLISTAALLRLINNFLSKQK
                     KLKQAQQVFSDLNLYLPGFVLLIMIVITLRQNYIWSDHIRFYEYTLRFTQSARLYNNL
                     AMAQADRGNVTGALENYQNALAISPGYPQIYHNIGNLLQTQGESVAALEYFQKALEVD
                     PTFTFSYSKALQLAIGLKDASASAQILQLAQTYLPEAYSEKLILMAE"
     gene            complement(23138..23211)
                     /locus_tag="PKH60_01170"
     tRNA            complement(23138..23211)
                     /locus_tag="PKH60_01170"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23175..23177),aa:Val,seq:cac)
BASE COUNT         6849 a         4411 c         5293 g         6563 t
ORIGIN      
        1 gatgagttca gcatgacaaa aagttctgag ggagtactac tttgtcagcg ctatggcgat
       61 aagattgaag ttgatagttt ttctaccttg acgggcggag gcggtggaaa cacagctgtt
      121 ggttttgcgc gagcaggatt tcatactgct gtggtaacag agacaggcaa agatgtgctc
      181 tcaaatctta tagtagactc cttccatcag gagaaagtcg ccacaaattt gatcatacaa
      241 gaaaaaaaag agcaaacggg gggatcggtg attctgattg gccaagacgg tgggagaacg
      301 gtgatggtgc atcgaggagc agcttcacta cttgatccag tagacattcc tgtggattca
      361 attgaaaaaa ctggttgggt gcatctttcg agtattgctg gcagacaagc aacgctggaa
      421 acaattttca aagcaaggaa aaaccgaaaa atgtcctgga atccaggtaa acaggaattg
      481 tatttgcttg cagaatctca actagcattc aatgctttac ccattgaggt attactcgtc
      541 aacgaacagg agtgggcaat ggttgatttg gttcaacccg ctcttacgtc tcatatcccc
      601 actattgttg taacaaatgg caaccagggg ggaagaatta tttcgaaggg tcaagagaca
      661 cattttagta gtcaatctat cccctcagtg gatgatactg gtgcgggcga tgctttcgcg
      721 gtgggttttg tgtgtgggta tatttggcag caaccattag atgaatgtgc tgtgattggt
      781 gccaaaaacg ctgcatcggt agttgggcac atcggtgcaa agcccggctt acttcgcaaa
      841 ggagatttgt tacactaagg atatccggtt tatatgtcag attttcgtca aatcctcgat
      901 caagaaattg cctcgctcaa agcaaagatt gataccgctg tgatgccaga ggaaatgagg
      961 ctgaaagttc aaaaagacat agttgcacta gaacgatcgg ttgagttggg gagttatgat
     1021 gagaaatatg agaaggtttc taaatatatc gactggatat tgcaaatacc atgggaaaaa
     1081 gaaagtgaag atcaactaga tgttgccaaa acgaaggaag tatttgaaaa gcaccatttt
     1141 ggcatgatgg atgtaaagga tagatttgtg gaatatgttt cggttttaaa gttacgacag
     1201 caacagtttg ctgatagtga ttttcgcgct ccagtcctgt tgctagttgg cttggtggga
     1261 actggtaaaa caacctttgc atattcttta gcggaagctt tgggcagaac catagtccgt
     1321 ataccgtttg gtggtatggg ttcagctaaa gaattgcgtg gagaatctcg tttactgttg
     1381 ggttcagaac caggcagaat tatcaagggt atttgtgaag ctggagtgcg aaacccagta
     1441 atcttacttg atgaaattga tagaagtgca gaagaagcca atcgagacat catgggtgtt
     1501 ttagtggagt tgcttgatcc ggctcagaac cacgcgttta gagataccta tgttgattac
     1561 ccggtaaatc tatctcacgc catctttttg gcaacggcca acaacacggg gaatattgct
     1621 actgcggtga tggatcggtt ggagaagatt agtatgccta gttatagcga tgaagagaaa
     1681 acagtgattg ctaaaaaata tgtgctacca caattacttg aatcggctgg tctaaaacca
     1741 gagcaattaa ttattgaaga tgctgtttgg ccagcagtag tgcgtccgct gggctacgat
     1801 atgggtatcc ggacattaca gcgcaccttg caaggcgcgg tgcgcaaagc agcccgcatc
     1861 attgtagaaa aaggctattc cgaggtggtc atcaacgagc agaaccggaa aattttctta
     1921 ccgcagtatt aataatctca ccttacttca agcaagcttt taattgctag ggcaaattca
     1981 aatggcttag gagcaaagtg tggctttgtt ctgactcttg ggtccgaatc gagataggtt
     2041 tctctctttt cttcccagat tccctctaaa gcataaatgt taaattcaaa ccatttacta
     2101 gcaatttggg ttaagaaata agaatacgaa ctgggatcgt cttcaacgac aaaccttgtt
     2161 tgatctaaat tatctctaaa atgattatgc acagagagta aaaaaagata ttgactgtta
     2221 ccaccttcgt agtcaacagc atcaagtaca agatgaaggg tgccatcggg atcttttaca
     2281 attaatccag gtgaaaaata gccattttca gctctactaa aagtggcttg taaatttgtt
     2341 agttgttccc gaagttcttg cactctattt ctggctactt cgatgtcctt tggatcaatt
     2401 agagtatctt caagtttatg aaagccatcg aatagcagtt gttttggatg atgatagctc
     2461 tctttgtctc ccctggggaa gatttgagag atttcagtac taattgagtc ttctgatatt
     2521 tttaagacaa attcagacac taagtcgaga tacgagtctg ctttttcaag taatgtaagt
     2581 ttattttcaa gcaatacttg aattttgcta tttgtttctt gatcagctaa ctcggcaaca
     2641 gctctttctt gttcagtgaa tggaagtgac atattaagat ttcctttgat gcattttcct
     2701 gcataaacct tgatttgtca aataaatgtg atcatttaat aacgcatcga tttttataag
     2761 ctacaataaa tactgtctat gtttgaaaaa aatcgttcgg cttcgttatc tcaaattata
     2821 tttgatctag aaaaatcagt tgatcaacta cccattgctg agaaacctac agatattgtc
     2881 tttggcgaag gatcaccaga ttctgagata gtattgattg gtgaggcgcc tgggtacaac
     2941 gagcacgtgc aacgtaggcc atttgtgggg cggtctggcc agttgttgcg taaaacgctt
     3001 actcaagtgg gttttccgcc ggagcaggtc tatatcacga atattgtcaa ggccagaccg
     3061 cccgataatc gtgatccatc tccagaggaa atcttagctt ataaaccatt tttagatcag
     3121 gaaatttcac taattgatcc aaagttaatt atcacactag gccggtttag tatggcgaaa
     3181 tttttaccag atgtaaaaat ttcgcaggtc cacggtcgtt tacataaggt tcgttggaac
     3241 ggccaaatta tgtatgtttt acccatgtat catccagccg ctgctttgcg ttccacccaa
     3301 atgttaaccg cctttactca agatttcgaa aaagtagcca agatcctaat ttgggtcaaa
     3361 gagcagagtg aattgattgt agaccttgag acaatatcag ctatactact ttaactagcc
     3421 gaagggaatc atccaatgtc accagaagaa ttgaaagaaa gaaacttcta tcaagagatt
     3481 aaaccagtgc ttgatcaact ggatgcagaa ttactgcgcc tacgtgaaca accactcgct
     3541 atagaacaca aagagggtgt tggttctcaa gctgctctag tgactgccgc ggatcgtttt
     3601 gccgatgaat atctacggaa agaattaaaa aaactattcc ctgaatttgg ttttgttacc
     3661 gaggaaggtg aacatcaagc cactgaagaa tatgtctgga tcattgatcc cattgatggc
     3721 acgaataact acgcccatgg cttagacatg tttggtgtca gtattgggct ttggcgacaa
     3781 gacggcacta gtgttttagg cgtgattgat ttaccggctc gtaagactcg aatatggggc
     3841 ggcaagggcc aaggggtgtg gcatgacggc aaaagactct ccacgccttc acagctggca
     3901 cctgaaaaag ccatgcttac catcgcacca atctggtcaa gtccagccca aggtggggca
     3961 gtcacacaag ctttggcaga aaaagtgggg cacacgcgag atcatggttc aaacgtctgg
     4021 caatcatttt tagtcttaat cggtcaggta aatgctaatt tgagtttctc tttatcaatt
     4081 tgggacattg ccgctgcggt cgcattagca ggggagctcg gattaagcgt ggtcttttca
     4141 aacaatgttc aacgacacaa cctaatacag caagccgatc aacccgtcac agtggcgatt
     4201 ggtcaacaaa ctttggcaga acaactcgtt gccgcagttg aaccgtggat atcctgagtt
     4261 aataccactt tcccagattt atgattggca gagagaatcg gaaaaccagc catgagctgt
     4321 taacgcgtgt ataatgacta ctatgaaaaa cattcttttt gggtttgttt gctttgtact
     4381 agtatcctta tttgtgcgac caatctttgc acaaggcaca ataggtactg ctaatccagt
     4441 catatggaag actgcctaca acgaattttc ttccagtaat ttttatattc aaattggcga
     4501 aagaactttt tatggatctg atgctattag agttaattct gatcctggaa ttgataaaac
     4561 aactttagag cttgagtgga aggaaaatga cattaccatg cgtttttatt tatattttga
     4621 aaaaattgaa aatggtatgt ggcaacttta tgacatgaga acgtataacg cgactggaac
     4681 cgactggata tattaccagc cagtagatag tctcggaaac tcagtaagca gcttgatcgg
     4741 acagcgaaat tatgcttatg agcggacgtt tttatctcaa gatggaagcg cgaaaatcta
     4801 ttgtaaagaa tgcagcatta ccgcatttat cagtaacata gcgccccctt cagttcacgg
     4861 ttacgtgata gattttcgca ttggcattcc tcaaaacgaa acgattacta tcaccaatga
     4921 accaaatact ggctatggcg taaatgctgt attgatggat gcctcaggtt cagtggttaa
     4981 agatcagtct gattttactt atagttggaa ggtagaaaac acttcaaccc tttccatata
     5041 tgctcaagga gtaccatacc cagatggcaa ctgtgcctat ggcatccttg caccatgtcc
     5101 tgacttcaat gtacaaatca gaggtattaa cccaggtatt tctagagttt tgttggacat
     5161 tactcgtaaa agtgatcaaa cagtagtggc gtccaacgca tttactgtga gagtaattga
     5221 aaaaaccctt tcaccgactc caagtccgag ccccactgtc aagataacgc caactccatt
     5281 tcaagatgat gtaacagcgc aattaacaga gcttcagggt gaagtgggta gacttacaaa
     5341 tgttgtagag aaacaacaag ttgagattac tgtattgcag agaattattc agacgataca
     5401 agaatttcta aagaatttat tttaaaaacg gcacaaacgc tacttgttgc cactcagtca
     5461 ccactggtgt ggataaaggt acaacaatag acgtaaatca ttaaaaaaca tagtagaata
     5521 gtaagatgtc aaaacaagaa aaacttattc aaatttcaaa ttggttttta tttttgcaca
     5581 ttatttataa attgggtgtg tttgtaggtt gctttgtatt gttgtggatg tttagctaca
     5641 tgattggtct gaatgaaagt accagtgagt catttagttt agttggagcg attattgtaa
     5701 ccactgtttt attactaaac aacattcttt actggaaaaa ttcaggagtc tatgtaacaa
     5761 aacaaggtga tcacattgtc aaacagggtg ggtggtttgt aaaagaagac aaattgttaa
     5821 acggaataat tattgctaat caggttacta gaaatccaat cgatcaacta ttggggatgg
     5881 ccagtattca gacgggttta tttggcgata aacacttaac cggtgtgagg tatcgtgata
     5941 ttcagagtta tgacgataca atgagaagct ctatgaatgg taccttctct agtatgtttt
     6001 aattacgatt tctatctaca agggtagatt gcttaggcaa atcaatcttc gttaccaaac
     6061 ggcacaaacg ccacctgttg ccactcagtc accactggtg ttgaagtatg tatagcaatc
     6121 cctgaggttt cataagtaag gggaagttgc actgcttgtc gtaattgttc agctgccttt
     6181 tgtttttgct cagtagttag tgtggtagtt gttgagtcag agccatcgta taacatacta
     6241 aagtgagctc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgtacggaat
     6361 tactgccatg aaagccctaa caaaatcatt atttcttaaa agacataaag atcctttggc
     6421 ggatttgttt caacaaacag agtatatcca tctttggttt tctcatctag tgatgtttat
     6481 tggggtcgca gctgtactaa cagttttggc ggcgatcaat ttgtttattc cttttcaagt
     6541 cgtcaactcg acgataatta ctatcgtcat gatagcttta ggagtggttt ttggtatttg
     6601 tagttatatt attttattgc tttccaatcc aaagcaagaa ggctatcgtt tgttgaaaat
     6661 agttaagttg gtgtatggag tattttttag tttttacatt ttttcttatt ttctaataag
     6721 tccgccactg gccacctctc actcatttta ttggttttgt ttatttattt ttgctacttt
     6781 tgttgtttgt agtttttcta cgtataaatc gcccagagct aactcatttc tcgagagggt
     6841 gacagatgta tttcgcgatt attcttgggg aaataatatt actttaatca agccaaataa
     6901 gcagagaaag gcataatgtc agaacttcaa ctacttccat taggtggaat gggcaccgtt
     6961 acccaaaata tgtttgtcta cgaatatgaa aacgagctcc tgcttgttga ttgtggtatt
     7021 ggttttcctg atatgcaaat gccaggagta gatattttaa ttcctgatgt tgcatatatt
     7081 ttaaccaaat tagaagcagg caagcgaatc gttgggatga tcttgactca tggtcacgat
     7141 gaccacattg cggcgttacc atatttgtta ccacatttgc ctcattttcc aatctatggt
     7201 tcgccgctta cagttggttt cgctcgtcaa agagtgagtg aaattacttt agaaccagaa
     7261 tttcatgtag tggccgaaag agaagaattt agaatcggta gtcatttttc tgccagaggc
     7321 tatcggatta cccattcggt tccagacact cgtcattatg ccattaaaac gccagctggc
     7381 cttatttacc acgggagcga ttttaagttg gatgaacacc cagttgacgg gttaacgagt
     7441 gacttggaac acattggtca actcaagcaa gaaggtgttt tactcatgat gctcgattgt
     7501 ttacgggttg aatatgatcg accggtcaaa tccgaaacaa cggttggtcc agttatcagc
     7561 cgtttgatga aggaaaccaa aggtaagttt gtggtgacat tgatgagctc tcatattcat
     7621 cggattcagc aagtaataga ggcggcggcc gaagaaggcc gccgcgtcgc ctttattggg
     7681 cgatcggtcg aacaaaatgt tcgcatcgcg caagagcttg ggcaactgca atataaccca
     7741 agcatggtgg tggacaagcg cgatcttgac caagtgccag atagcaaagc ctgtttgatc
     7801 gtggcgggaa gtcagggaca agagggttca tcgttagttc gtgcgatttt tggcgaacat
     7861 cccgttttcc agataaaacc aacagacaca gtggtttttt ctgctgatgc cattcctggc
     7921 aatgaattag cgtattttgc cgcgattgat caactggcta gcaatggagt acatgtgttg
     7981 tatccagatg tggaagcaga tctgcatgaa tcaggtcacg ccagtcgagt agagcaaaaa
     8041 cagatggtag acctggtgag accagaattt gtaatgccga tcggcggcgc cgatcggcac
     8101 cgcttcaaat tcaaagaatt tgtagccgaa cccgcgggct atgatgccaa acacacactt
     8161 ttgccagtct ctggcgaaat cttgggcatc tcagcatctg gaattagagt ggtagataaa
     8221 ataaccctca agcctccagt ggtagacggt ttgggtgtag gtgacgttgg tccagcggtg
     8281 ctgtcagatc gtcgcaacct cagccaggca ggtatggtag ttgtggtcat tcccaagatc
     8341 cacggcaaac ttgctgtaga aaaaaccgag gtaatttctc gaggcttcgt attcatgaag
     8401 gaagccgagg aggttatcga ttttatacga gatgttgtgc gagaaacagt ccagcagaat
     8461 caaaaaacta acgagggtga tctcaagcga gcgatagaaa agagattggc gcgaaaattg
     8521 tataaaataa ttaggcgaga gccattaatt ctgccggtaa ttttgccaat ctcttgagag
     8581 aaaataatca gccacttgtt ttagagctaa tcttatgata tatcctataa tatgaagaag
     8641 cggagtaagc gcagacatcc aaaattaaaa atgaagcccg aaaccacgca ggcaattgtt
     8701 ggtgttggcc tgattacgtt cggattgctt attctgatct ctttttctgg tcagggcacg
     8761 gccttaatcg ctatcaacaa gtttttagta gaaaaacttg gcgcatcatt actgctactt
     8821 ccatttttat tgattgcagc aggtttaagt ctgtttcgaa cgaaatttcg ctggactcaa
     8881 ccacacgtac tactgggtag tttaatttta ttcattggtt ttgtcttgct tttccgaaca
     8941 gggaccattg gtctgaatag ttttgaaagc acgagtcgcc taatcagtaa ggctggtact
     9001 atcattgcta gtatttcact cctgttaatc ggtattttag ttatgactca gttgaaagtg
     9061 caggaaatga tagatgtttt tggtttatta aaacgcaaac caactaagtc aaccgaaaaa
     9121 gatactgtgg atgagaagca accaggtcgt tttcatttac catctgtcca tttgccagca
     9181 ttgccatttc gatcaaagga aaaaactccg gatgaaagat ttgttgatga tgatggtgca
     9241 aaaaaaacta ccaaaccgcc actcctggaa cctctgatca ccccaccacc gaaagagact
     9301 ccaccaacag agtccttacc aaaaagtacc aacattgcgc cctccactac aatttggcat
     9361 ctgccaccca ttgatttgct tgaggaagca aagggagtga aggctaatcg gggtgatgtg
     9421 agcaaaaatg ccgacacaat tgagcaaacg ctggagtcat ttggcattcg tgcagaagtt
     9481 aaagaagtca atcgagggcc agcggtaaca cagtacgcgc ttgaaattag taggggcacg
     9541 aagttatcta aaataaccgg tctctctacc gacttagctt tggcactagc agcaccaacg
     9601 ggacaaattc gaattgaggc gccaattgct ggccgctcat tagttggaat agaagtacca
     9661 aactattctg ctgaatttgt taatttgcgt gacatgcttg aaaaaccaat catgaaaaaa
     9721 caagccaata aaaagttgct tgtagcgttg gggactgacg ttaccaacca gccagtggcg
     9781 gttgatatta acgccatgcc acacattttg attgccggtg ccactggatc tggtaaatca
     9841 gtagcgctca atagtttctt gtgctccatt cttttccgcg cgacaccaca agaagtacgt
     9901 ctcattttag tcgatccaaa aagagttgaa ttaacctggt ataaggagat tcctcactta
     9961 ctcactccag tgattgttga aagtaagcaa gtggtagctt cactcaactg ggcagtgcat
    10021 gaaatggaaa atcgttacaa acttttacac gaagtgggag tgcgggagat tgcggggtat
    10081 aacgaagtag ctggtcactc agctatgtat catattgtca tcgtgattga tgaattagct
    10141 gatgtgatga tgtacgcgcc cgcagaggta gaagaagcgg tcacccgtat cgcccaaatg
    10201 gctcgagctg ttggtattca tttggtcttg gccacccagc gtccttcagt tgatgttcta
    10261 acaggtttaa tcaaagcaaa tattcccgct cgtattgctt ttaatgtgtc atcaatgact
    10321 gactcgcggg tgatcttaga tactcctgga gcagaaaaat tacttggcaa gggtgatatg
    10381 ttgtttatct cgccagatag agccaaacca attcgtgtgc aaggcaccta tgtttcggac
    10441 aaagaaatta aacgcctgac tcaatattta cgcgaccaag attttgaacc ccagtatcta
    10501 gaagaagtta cttcaaaata caaagtcatg cctggtaaat ctggagtctt agatactaat
    10561 ggtgatgaaa aagatgagct ctttttggaa gtgtgtaaga ttttgattac tcaagacaaa
    10621 gcttcttctt caagtattca gcggcgcttc tctattggat ataatcgcgc cgctcgcatt
    10681 ctggaccaac tctatgccgc aggtatgatc ggtccatcag atggcagtaa accccgtggc
    10741 atcaatgcca cgagaataat ggaatacatt catcatgaag aaggtacgaa tggttcgagt
    10801 tcagaaaaca atgaataact tgattgagcg ttgtatactg gagtagttgt agaaaaggag
    10861 acagatgaaa aaacaatacc tttgggcaat agtagtaatc ttagtgttag ctggagcaac
    10921 attgtttgtt acccgctcgc aagatttgcg gatgccagtc accatgccag agtttgttaa
    10981 tacacagtct acatcgaatg attcttttgc aattggagct gaaccagcgg gttatatgga
    11041 aaaaggcgtg atggtgagtg atgtgtatca accgatgcca ccctatattg gtggtggtga
    11101 tgcactaact gttgaagagc gtcagtacca aacctatgcc tcgtttagcg tggtggtgaa
    11161 agatgtgagt ggatatctca atcaactcag agaaaaatac ctaggaatgg aaggtcgggt
    11221 gctaaatctt tccaccaata cttcgggcaa atatcaaaat ggctatctta ccgctaaagt
    11281 accagtggcc tcattcgatt ctgctagtca agaggcaact aagggtgtag aaaaaatagt
    11341 tgatcaatca gttaattcta ctgatgtaac tggtgtagcg gtaagttttg aagagcaaat
    11401 tcaacagcta gaaacccagc gctcacagcg agaggttgat ttgcttgagg ctaaaactga
    11461 tgccgagaaa aaacgtattc aattggatat caacaggtta caaaatcaaa ttgagtcgct
    11521 caagaaacaa aaagtaaatc aacaaactca ggtgcagtat gccactatta cggtttctgc
    11581 agcttcttca gaaaaagtat tcaatccttc aaccagacct gatttggggg aaactttgcg
    11641 cgaagcggtt gattcagtgt tgaataatct atttgaagta gcgcaattct ttatttgggc
    11701 cgcagtgtat gctgtagtac tattaccagc gttgtggtta atcggtaaat tgcgcaagcc
    11761 aaaaaccact gtgacaacgg aataaagtgg gtgtatacta ccatctttcc tttcaagaac
    11821 gttcgggaag cgatctgacg acagtcggat tgttttagta gtcgtactat gaaaaacata
    11881 ctagtggtca accagctcaa aaaaatttat ccgcctgcaa caaagcgcga gagtgagttt
    11941 tgtgctttgg atggaattag tttcacagtc aaaaaaggtg aaatcgttgg tctactaggt
    12001 cccaatggcg ctggcaaatc taccactatc cagatgttaa ttgatacgct caagcccaca
    12061 tcgggcacga ttgaatattt tggcaaagat ctgcaccaaa atcgcagcga agttttacag
    12121 catgtaacat ttgccagcac gtatattaag atgccttggc gactgacggt gatggaaaac
    12181 ttgtggatct atgctttact gtatggttta aatcgagaac aatttaatca acgagtagaa
    12241 agctttttaa cattctttgg cgtgtgggat cagcgacata aaaccattgg catgctttct
    12301 gctggtcaaa ttactcggat aatgttggca aaggcattta ttccacaccc gaagattgtg
    12361 ctattggatg aacccactgc ctcattagat ccagatatgg ctcatcaggt tcgtgatttt
    12421 gtgaaacgac aacaaaaaga atacggcatg agtattatct atacttctca caatatggat
    12481 gaggtggccg cagtgtgcga cagagtgatt tttttgaacc atggcaaaat tgtggcgttg
    12541 gatacaccag ataatttggc gaaatcagtc tctctggctc gcgttagact ttgggtcaaa
    12601 gatggtttga agcgaactga acgctttgcc aatgagcaag gtttggtcag tcacattgaa
    12661 gacagggaaa tcgtaattga aatcgatgaa ccagcaattg ctgggtttct cgcttcgtta
    12721 gccgatgcac aaattgagta ttctcaaatt agtattgata aaccaacgtt ggaagattat
    12781 ttcctgagta tgagtaagca ggaacagaca aaatgaacat agccaaacat agactacaag
    12841 cgttatttac tagacattta tatccactca agcgtgactt tgatctgctt tcggatatga
    12901 tttattggcc actcatcgat acagtgttgt ggggtgtaac tggcatgtgg ttgactgaaa
    12961 cgagtggcgt gcccgagcta gtagtggcgc tactaatggg actcatcatg tggaatatta
    13021 tttggcgctc acagtcagaa gtcggtcgta acatgatgga cgaaatttgg aattcaaatt
    13081 tggtcaatct cttttctaca ccagtttcaa ttctagagtg gttgatttca gtgattggct
    13141 tatctttcgt gaaaacgctt gttactttga cagtaattat tcccgtcatg gttttgctgt
    13201 actcaattaa tgtgatggcg ttgggtttgt ggttaccagt attttttgtg ttggcaagta
    13261 tgactggttg gtcggttggt ttattggcct ctggcattgt attgcgttgg gggccgcgag
    13321 tgcaaacggt gatttggacg ttgccaggaa ttttgttgcc gtttagcgcg atctactttc
    13381 cggccgaacg tttgcctgga gtattacaaa caatttcttg gtttgttccc actacctatg
    13441 tgatggaaac cctgcgtgcg gttgttttca atggatctcc agaaatcatg atgctggtta
    13501 tcagttttgg actgaatatc ttctacttag tgttagcctc gattttcttt ttacgcagtt
    13561 ttgaatacag caaaaatatg gggctcaatc gatttacata atcttgttta caagctttct
    13621 aatcatgagt atgcttaaca ggtgagaaga tttttgacgt attttcaacg agtgtctggg
    13681 ggatatttac ttgcgctcat ttgtttaatg gtattgagtg gtgtggttac atatgttgtt
    13741 tgtcagtatc aaaatcaaac cattcgttta agaaaccaat tcttagaaca gcggtataag
    13801 gaacaaatga agaatgaagt tgatgcgaga caacctacgc gacaaccagt gaaagactcc
    13861 gtcgaaacag aaaggatcta tgaatctcgt gatggaaaat tgctgactaa gatctattgg
    13921 tcacccaatg actatggctt agatgtagag actattgatt tagataccaa agttgttaag
    13981 cgtcattcag aaaatgatta tttcaaatta atcaagcgtg ttgaaggatt attttcgaat
    14041 agtctcgaat cagtattctt attaacaaga gatagtaaac agtacgcagt tattgatacg
    14101 tatgttggca atgtggatgt tggtgatgga ttgcgggaaa tatatgtggc aacatttgaa
    14161 ccaatcaaag aatcgttgac aataaaatca ttttctatca cgccagtttc tgcaaaaacg
    14221 atttctatta gtagagcata tggtagagtt gaaatacttg actatttccc acagcagaat
    14281 caaattataa ctatttctag atcatcagat ggttgtgggg gaatgggttc ggtagaattg
    14341 ctatcagtaa gtattcaaaa ccaagtagta aaagaagttg gtatgggctg tgttacgcaa
    14401 acaagatact tgggatattt gggcaaccta ctttattttg gagaaattga tctgacggat
    14461 gaggatctgg aaaactggga agatgcaaaa atcatcaaca ttttcagtct taatccactt
    14521 actaaagagc gaaaaaatct atctatcgat ttgtccacgc atgcttttga tactacttgg
    14581 gtagatgtaa caaataaaat ctctgcgact gaacttgtgc tttttgataa aactactgag
    14641 gaagggtatg ctttggatgt gaagacactt gaactgcgaa acttgggtaa gttgtatgat
    14701 tagttttcag atatgcgata tactaagagc cagtaataat tatcggattt tatagtggca
    14761 gaagttccta caataaaaga aaagagtata gtaacaccgc ttcctgaggt tgtgatacct
    14821 gagtcagttc gcgctcagtt tgcactagcc ataaaaagaa ttatgccact atttatggat
    14881 tggaacgtgc atgatgaatg ttgggttgat tcatcagtaa aacctgattt gattattact
    14941 cttgcacgag ctggatcact ggcttgggta ccaatggcag caattgctga cttatacgac
    15001 atccgattgc cgaaaacttt tgatttacat ttgggtagag agttggcata tcgttttatt
    15061 cgggaaagtc agggcgattt tagattactg caaaacggtg atgccaaaac tattgcagat
    15121 tttaaaaagt ggttagataa ggagtttaat ctaaatcctg atttggcgga ggcaattgct
    15181 aatatatcag aaagcttggc agcgaatgat aaatcaaaaa atattgttgt atttgatgat
    15241 gttgtgcaag attgtataac aatgggaata ataattccca gattgattga gcggagttta
    15301 gcaacaaatg tgacattcgt gtcatcgaat gaaaagcttc aaaagcgttt ggcaatcaga
    15361 gatctttcat ctgaaattgg gcagacgaca aaagtaacct tagagtgcat tctggatgat
    15421 actgagtggt tgtatggaat catcgataat tcattttcag agtattttga ctcattatat
    15481 ttagaaaaga aaattgttgt acgtcagttg ttggtacaac ttgcaaaagg ttctattgaa
    15541 gatattcaaa caggtgcatt gaatttgata acgaatgagg ctcaaataag gaaaattggt
    15601 gaaaacattc aacagcagta tttaacaaat aatgccccac gacacgaagc aatgtttata
    15661 gatccgggta atcccgcaga tgaaatattg gaaacgtatg gtgttactgc gttgttaaca
    15721 ttacaaattg cagttagaga agaattggaa aggttgggcc agaccgtaga actgtgaaga
    15781 actacggtct ggccagagaa ttactcaggc ttgttgatct ctactgggga gtgcggaatc
    15841 cccaggtagt ctagaacttc atccaagatc attaggatga ggttccgctc gattgggtta
    15901 cagggttccc agtgggttgc tgtaaccatt cgccagtctc gatcgaactc ttccaagtcg
    15961 tcctcaggga tgttacccct gaggaaagta cgatcattgt tgtcgagatt atggagaaag
    16021 gttaccaagc cgctggcggt ctggtagacc agtgctctcg cggagcccca tcttccaggc
    16081 tccggacgct ttttccagca ctgaattgga ttggtcatgc cgatggtcgc aatctgactc
    16141 gcgtctgcgc ggcgcatttc tggacgacca attcccgaat tggtatcgtc taggtgcccg
    16201 ttgccccgca cgatggcgta atcgtgcagg tactcgggcg ccaatccttg agggaagatc
    16261 acgccctccc tccaggttgg aacgagcaca aatccccttg agaccagcga tgactgaggt
    16321 cgctcgatcc agatgacctc aacactgtct tcgggaatcg cgccaccgct ccaaggcaga
    16381 acgaagtaac gccctgccag gctgaaactt gacatcttgg tcgccgtgaa ttggttgtat
    16441 cgtcccaaag ccgtgttcat gggatgaact tggcttgata cgataatagg ggtgtttggg
    16501 gggatatttc gtaatcccaa ccatcccagt acggttacgc caacaatcgg cgccgttgtt
    16561 ttcacgattc tcgctaggga taccccctca cgcgccactc ttcccacgac gattgcatcc
    16621 ccgatgggga gtggtccgtc gagggctatg agagccaggt ttgagactaa gaaaaacgac
    16681 cttggctgat caaacagcgc ggtcagaccg ttttcttcaa accaagcttg atccggatct
    16741 gatggaatgc agaccggatc gccgatgtta ataaaaccat tcaaggctga attgccttga
    16801 atcaaaatgg tctcatgtac atttaccctc gccgcaaggt gcggaaaggt ctcgacgatc
    16861 tcgctccagt agccccacca cccagggtgg cagaccaccg gattctgctt tttcacaatt
    16921 gggagaaata ccttgcaccc tcccgctggg cagggccggg gggttggcca tggggttgca
    16981 gttggtgtcc gcgtcggaga tgccgttgga actgctgttc gtgtgggagt ccgcgttggt
    17041 gtggcagttg ccaccgtcgt ccgcgttggc gttggagtgt tggtaggcca aggcgttgac
    17101 gtagccggtt gagttggcgt gggagtcgta gtcgatccct gagcaggtat gtagtatatc
    17161 ccccaactat caagatggaa tggtgactca acagatcctt gctggtcact gacgatgtcg
    17221 aggccgaacc tttcattaac tcccaactct ctgaaatcag aggttctctc cacggcgtct
    17281 tcgatccaag tgggtcctcc taccacaaac gctgcgttat ggccaagaga aaacctcgcc
    17341 ccatcgctta atcgtctgaa ataccatctt agacgatcaa tttcagccaa acctgggttt
    17401 tgattaccag agtctgccct gattttgtac gttaaataca cctgagcgtt ctcaggtatc
    17461 gatgggactt gccagttcca ttcatacata gagtaatttc ggggtgcgcc gttactctga
    17521 tgcgcataat ggtatagtcc gcttgctgag ttaccagaaa tgcgcaccca gtcgtgcagt
    17581 gagctgggat cggtctcgtc ccggaacatg acccagactg cattggctgg aacacgcacg
    17641 ctctgacctg ataaagtgat ttcttcatct tccctaccca gtgggtccat aatgggcgga
    17701 tcgaccgcag ggactcccag ctccaagtca aatgggggat tttcactagg atagaatgag
    17761 gaatcctcct gcgctacagc tgcaggactc accgccggca tgctgccgac gacaacgatg
    17821 gccaacagaa gggcaaacac aatcgccctc atccagaatt tggtcgacat ttctcgtgtc
    17881 tccttgaacc gaagataaac ccaccaaagg tttggtgagt tacaaccaga aactggctgc
    17941 gcagtgttta caaaaaacta ccaataaaca cagcgcagcc agttagttgc caacagagca
    18001 gaaaaagatt aataaaataa aatagaaata agcctgagtt gttaaagagc atcgagagag
    18061 cactagaaaa atcgcgatga tttttagtac ttcacgatac acatctatta tacattataa
    18121 gacgtgaaaa tacaataaac ttaacctata acatataagg tcactattta cgtgctaaga
    18181 taggaagaat gaaaaagatg ttttttacta ttttgttgct aatgttactg ccaatttctg
    18241 tatttgccgg tggtggacgt attcttatct ataccagcga taaccttggt ggttattatt
    18301 atgtgaactc agatatgtat gttttggctc aagtattcgt gccagattct agtaatacta
    18361 ttgcctctat tggtgaacga gccgaattcc gtatcgaaaa tcctcgagca ggcgattcgt
    18421 gtgtaaatga aatcgagcaa actactgagc tcgggttact cagagctcgc tgcagagtta
    18481 cacagcctgg catgcttact gtgtacgtgt attcctttgc agacaactat gagtcatctc
    18541 gctacatact taatttttat gctgcgccaa gtcctacaac tattccaaca caactaccaa
    18601 ctgtgatacc cacgactgta aaatctactt taactccaac aattgcagtg aataactcaa
    18661 tcacgacagc tcccgtagaa ttgactccaa ctccaactag ttcttctgat agtaacgctc
    18721 tactaaaaga ctatgtcttt gaagaatcgg tcgctactgt ctcatcagag gtggatttag
    18781 acacaaattc cgaaaatcgc attgctcaaa gcactgaaag taggacaatt gtattatctc
    18841 tcgcgctact tatgtttgga atatgcatcg caggtgttgg attaattatt ttcaaaacat
    18901 ccaagaaaac ctaaaaggaa tcgtttaaca aatggataaa ttaggttgct aaaagaagtc
    18961 tttttgtcga gatgcttggt aagcagtgga tcattttgtc acttgggtaa ttaggtggga
    19021 gaatcaactt gataaaagtt gggcatcggg taatttactg actggaaaca aatatattgc
    19081 ttacatccag gtatgtgaaa taaaaacaca aacacttgtt tctgtagaaa ggtttatgta
    19141 tcatgcaagc tgatgcatag attacaataa ggattaaaga aaatatggag tttttgagaa
    19201 aaatagttaa taggaataaa agagaatcta aagatgttga ttatccgaat gaatttactt
    19261 tagaacaatg tataagtgaa ttaactgggg atggatttac ggttagcagc gctctccggg
    19321 acgatgtggc aagatttggt cttgtagaag gtattgttct agctatggat ggaaacagtg
    19381 gattaagggt agtcaattgg aatggagaag gattggtaat taaaacattc aacggtttga
    19441 gagacggcaa agatctattg tcgaaattgg caagagttaa gtatgattcc aaaccaatcc
    19501 atctctagat accatagaga aattcacaac agttttttgt ggagatgacg ggggatgacc
    19561 ccgtgtccgt gtccagctaa atagcagcat ctacaggttt agttcctctt tgagtgtgga
    19621 gcgccctggt gaagaaacaa accgtggagc gatccctttg tactatggct tacgactact
    19681 cggtacaagc aagtaatcag tcacactggc tgagttttca cccactcgct cacaccagtg
    19741 agatgaaacg agtgaatgcc ttgttatgct actagagcat aatttggaga tcgaagagat
    19801 tttccaaaat tggaaaaagc accttttact tcagttgtta ctgttgcaat taatgctttt
    19861 gctctttgac cgttactgta gattgtatct gcagttatag ttttggtcct gttaacgtct
    19921 tttgaccaaa gacgacctgc gactgttaaa gagcgtgtca cgtcaaagca acacatcccc
    19981 attgggacaa tcagtatagc aaaaatctac gattttctca agactaactt gcttgacgag
    20041 caactgtcag accgatcacc gttttacatc catttacttt tactatgcgc gcacattcgt
    20101 taagcgtaga cccggtagta agtacgtcgt cgattatgag tatcgttttg ggaattgccg
    20161 aagtaagttt tggattgagg gaaaagtggt taacgacatg gtctaaccgt tcatctctgg
    20221 attgaatcga tgcttgatga gagccgtgga tggttctgat gagtaaatct tgataaggtg
    20281 tgttgactag ttctgccaaa gtttgagcga ttacttgagc ttgattaaat ccacgctcgc
    20341 gcagtcgcat tggatgaata ggcacgctgg taatgcagtc gactggagga atgatggtgc
    20401 agtggtagag gagccggccg cagaggcggc cggcgtcaat caccgattga tacttgagtg
    20461 acttgagtaa ggccttgata gcgccgccgt aacgacaggc gctaatcaca tcatctaaat
    20521 acagtggggc gagctgtggc aggggttgct catacaaaaa atcgatcgtt tcgtaacatt
    20581 tgtcacacaa cagctcgccc cacctttcac aagatacaca atacgttggc gcaagaatgc
    20641 caaatactat tttgctcaac agtttaataa acaacacaaa tttaaaatct agtacgtaaa
    20701 accgtaatca accgtagaat taaaaactga atttttactt actctgccat taatataagt
    20761 ttttcgctgt aagcttctgg caagtatgtt tgagccaact gtaaaatttg tgcactcgca
    20821 ctagcatctt ttaaaccgat cgctaattgc aaggctttgg aataggaaaa agtaaaagtt
    20881 ggatcaactt ctaaggcttt ttgaaaatat tcaagcgctg caactgactc accttgagtt
    20941 tgtaataaat tgccaatatt gtgatatatc tgcggatagc ctggggaaat tgcgagcgca
    21001 ttttgatagt tttctaaggc tccagtaaca tttcctctat ccgcctgagc catagctagg
    21061 ttattataga ggcgggcaga ttgtgtaaat cgtagagtat attcataaaa tcgaatatga
    21121 tcactccaaa tatagttttg acgcagagtt atcactatca taattaataa aacaaagccg
    21181 ggtaaataca aatttagatc agaaaagact tgttgtgctt gtttcagctt tttttgttta
    21241 gataagaaat tattaattaa tcgtagaagc gctgctgtac tgatcaacaa tccaacgata
    21301 gggatataca gccagtgttc ataaaataag ccattcactg gtacgatgcc agaaattggc
    21361 actaatccga ttacaaacca aagtacacca aagccaagcc acagagtttt tagcttgttt
    21421 ttttctagga tactcaacaa aactattagt atcgaaacaa gaaaaaccga aattgtccaa
    21481 gggtttagta tagagtgaat cactggcaaa acatggtcca tgtgtaaagc atagggaaaa
    21541 gcaaatgtgc caatataacg tggtaaagct cgagtgaagg tgagtaatcg aactgatagg
    21601 ctggaggggt attgtgggtc gatcacgctg tagtcaaaat ctggatcgaa attcagcgtg
    21661 gtttggcgaa cataggtata acccaatccg agaattagta aaaacacaat agcgatggta
    21721 gtgggaagat attttgaaaa tataactttg attgtttgtt gtgtttgaaa gaggtgaagc
    21781 aatagtatca aaccatacaa gcctaatccc gacagggcta attctttgga taatactgcc
    21841 agaatcagcg aaagcgcact ggtgaaaagt agaaatggtt gagaaaagct gatctgtaaa
    21901 tcgtaaagag aaaaatgaag cttctttttc tgaaagctga gccaaaaact attgaggcca
    21961 aacagtaaaa atacagtaga gagtgagtcg ccgcgagaac taatataagt aactgcctcg
    22021 gtttgcacag ggtgcagtaa aaaaaacgtc gttacccaaa agcgccagcg gttagagaga
    22081 cctaaattta taagtaacca aaaaaggagc agaccggcag tcagatgcag aactagattg
    22141 gtcaggtgaa acccaatggg attgttgccc caccagccgg tatcgatagc gaaactaatg
    22201 ctagtgagcg ggcggtagta gttactattg atgccaccgc ccgcagtggt gctttcctta
    22261 aacatttggg caaaattgaa agattgaatt gtctgatttt cagtcacgaa ctgctgatca
    22321 tcccagataa agggactatt cacaaaagga aggtagcaaa gcgcagtcaa acaaaacaaa
    22381 agtatcaatt gatggcgaat tcgcatggat caagtataac agttgtgggt gtgttcagag
    22441 ttttatacaa tgaaatgagt agtgttagaa caaacgttaa aattacaaaa accagactta
    22501 gttgattccg aagcaactat ttttagagtc tttgaggatg aaagcctagc aggaccgaca
    22561 ggaaatagag gcatacattg gggatttttt attaagagtc tcctgaaggg tcagattgaa
    22621 atagctggta ggcacaggaa tgtgagacta gttaaaaaag tcgaagaaaa taacgaacaa
    22681 tattatcgca attggaggtt gcttaagaga ttgggaattc caacggtcga ttcaatgcga
    22741 acaagtggaa aatggtacgt cctaatgact gatctttccg ctaagggagc tgagagctat
    22801 ggcaaagaac gatttattaa ctggaagatt ttacattcgg caatgtatgt tggagtggaa
    22861 aatacattag acgacatctt ctctcaaatc tcctttgatg aaattatcgc agaagttcgt
    22921 cggattgaag cgatagcgtc aaacaacgaa gtgctactac caggagatga tccttttgat
    22981 ttgttcattt atccaagtgg aagttgggag gttctggtat tggatctcac caaccttgtt
    23041 taccaaccta gtggtttaac tcatgaacaa ctcaaaaatg aaaactctaa gagggctata
    23101 aactttttgg aacttattga gtatatgaaa aacaacgtgg tcgcaccagg actcgaacct
    23161 gggacctcac gaatgtgaat cgtgcgctct aaccaactga gctatgcgac cactct
//
LOCUS       DAOQZP010000010        21301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_2837_length_21301_cov_6.293326, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000010 DAOQZP010000000
VERSION     DAOQZP010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 21301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21301
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2837_length_21301_cov_6.293326"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="PKH60_01175"
     CDS             complement(<1..952)
                     /locus_tag="PKH60_01175"
                     /inference="COORDINATES: protein motif:HMM:NF039533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNQ16694.1"
                     /translation="MPGLESTVHDRFSQKKWVVSTLILIPWIFCSNFYQAQAQVQIST
                     SSASLVSATVPDIESPSIPVLISPEDEAILTTSTPTFVWYGSTDNVGIDHYDFWLNEV
                     THISNISPVDQDTAIFTLVVNEDNNTFSLTIKEAISDGDYTWSVVASDAVSNTATSAT
                     WDFSIDTLAPYLSITKIEDETVLITTANSQTLPTEPFSTKHKDPLISGLGEPNTTLLI
                     ILAIPGQNTVSTTYSIGSGGTWTYQFPSLDKNTLVTLTLIIEDSAGHTNTLTPIQFIY
                     KSLTETLVSPTVEAPTTTSHPKPTIYLVALTPTPHPTLRPS"
     gene            complement(961..2028)
                     /locus_tag="PKH60_01180"
     CDS             complement(961..2028)
                     /locus_tag="PKH60_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16695.1"
                     /translation="MHCPTPKRTLRSIITTFLFLFSVVVSFSFVKVVLAQTETPVEPA
                     TTTSQTTLTAIPPRLGDDGSLTASPGEVLQDTVRVRNTSDKPLTILSSVHDFVLDVDG
                     STPIAVTDQNLSNRWSLASWITIAPNLQTIQPNETKIISVIIAVPDDALPGGHYAMIT
                     HQPSTEAVPKDGETADTKLSATGISQRVGTLLYVNVKGAINESAFVRSFNIPKLTEYG
                     PVPYSFTIENQSDIHIQPGLMLEFRDMLGRKVDQVAIESKNIFPLTERRFEGSWEKVW
                     GTGRYTATVTMNYGSQGAIAIAKTSFWLFPFTLVVAALLTLLVLLAMFIAVRRHLIHR
                     KTDQSAKIKELEEKLSRLESK"
     gene            complement(2053..3528)
                     /locus_tag="PKH60_01185"
     CDS             complement(2053..3528)
                     /locus_tag="PKH60_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16696.1"
                     /translation="MKILSVSSVQRIGLATCLVLGMFVVGHLQGTRSANLSNVSVTLS
                     SSRPSFRGGLASGNTVGSSEVILNTTNGVWPSSSSAQLVVGDSLRIGEAGSLGTYTIT
                     EIVDNSTVNVTPVLASGDTELNDDVVASTSATLTVRLTTANAIANGAFRILVPADSTD
                     AAARDGIPDGTGFDFGTSAPTVTCPSSAPATYLFATGTATASAVTVNGTKYHSFECRY
                     YGTGAIGTVFDGTTYGAFTITSLINPAPSRTGHTIGYADTYNIIVRQVDSTYNVADAT
                     TVNVGVVESVRVTATVSPQITFRILGVNSGTSVCGVNTDVSTTASLVPLGSLSISAFT
                     TAAQALSVSTNAPNGYAVTTVENDQLGRNGGVCTGDNTGVNCIRDSVGDGGAMSHTAP
                     AKWSLGTAKGFAYSLHEVNAGAGTPAFSYNTNSGNCDGTNYCFKQFADAENSQAPQSI
                     FTGTTVADNDNVFVCYKAIIAATQAAGEYSNYLTYTATATF"
     gene            complement(3598..4575)
                     /locus_tag="PKH60_01190"
     CDS             complement(3598..4575)
                     /locus_tag="PKH60_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16697.1"
                     /translation="MITSNKIKTWHLVVVFCGISWFWLQYPTSISAQSDYSLSVYPPS
                     AYLKIKPGTTANHTIELTNTTTNTIVVTPKVFDSKPEEDSGYPEMQPTFSFPYLSEQV
                     QKMSPILIKPGETNSFSLDFTVPPEAITREYPLTVLFSSTIEQANTTNQVVSNSKLYG
                     VIGANLVVLVSSDETLPNLLQVVDIEVPRLLDSLRGLSFTPMLINQGVQTVVASGSAT
                     IVDTFGRTVFQAQIYPDMILGRQQRSARALTTQKDSKLPEPTVFSYDPLFLLGKYTIT
                     TKIIDGQGNSSSSHTKTVIALPYSFLIVVLFIIIGWVFSRVFATNTSPD"
     gene            complement(4595..5362)
                     /locus_tag="PKH60_01195"
     CDS             complement(4595..5362)
                     /locus_tag="PKH60_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16698.1"
                     /translation="MNLSNPTHPQMVLYGFFGTLLVLILICLSSQAVYAETLESNSFI
                     IQFGNFNVTSGEKSSTTYNVTDTVGQTGAGPYGEFGTASYFVGGGFQYIYPIDQFRFT
                     ISKTDINLGQLTAQAHNTDTNVLTITAPTAGGYQIYAYEQHPLKLLNGIPTIPDTTCD
                     NNDCDQTTAEIWQNTDVGGFGFNVNGDDTAADFINTNYFRQFANNALSEPMQVIMSNT
                     GYVVNRAATVTYKAGLFGSEPAGTYETSIVFVAVPAY"
     gene            complement(5349..6104)
                     /locus_tag="PKH60_01200"
     CDS             complement(5349..6104)
                     /locus_tag="PKH60_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16699.1"
                     /translation="MHPRQHTALLLFAIFLAFVWLQVPMLKSLSLQAVAASTICYFVI
                     KKYTRAKLKHILPQENSAEMALVTFSLLLLVGNSGTLNSPLFPLTYIHLFFLVMSSSR
                     STAITVGPATALFHFGLEPTISLSNIGHLITIPIILLFFLFAREQHEEVIKEKQTIAQ
                     EEVLLTQCSKETRKLQDKLAKLEKDKNNQRSQLIEGRALLDQARQQITQWKQQYFACE
                     PKALAEADDLNQQIDQAILLQPSVEEKTENELV"
     gene            6169..8679
                     /gene="leuS"
                     /locus_tag="PKH60_01205"
     CDS             6169..8679
                     /gene="leuS"
                     /locus_tag="PKH60_01205"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNQ16700.1"
                     /translation="MKKPIPQSYNPSESEKHWLSEWEKIGLFQSTTKELSEKEKFYLL
                     FAFAYPSGSGLHVGHVESMTALDILARFNRMQGKHVFFPVGWDAFGLPAENYAIKTGV
                     APAITTKQAIDTFHEQIKRLAISYDWSSEIATCHPGYYKWTQWLFLQLHKQSLAYQGT
                     GTVNWCPSCQTVLANEQVVDGLCERCGAAVVQKQMKQWYFKITEYQDELISGLEEVDW
                     PHATKQQQLNWIGKKTGITITYQVQNQSGENAGEISCFTTRPDTNFGATFVVIAPEHA
                     LAQALAKTSPEIAEYVEKTLHKTELERQQEGRVKTGVDTGLMAVNQLTGRKLPIWVSD
                     FVLAGFGTGAVVGVPGHDKRDFEFARTFSLPILRVVKAADGDISEITKLEQVQESEGV
                     MVNSGFLDGMAISEAIEAMTAHLEKQGWGKRVTTYKLRDWLISRQRYWGAPIPIVYDP
                     EGQPHPVKEEHLPWLLPTDVDFKPTGESPLQSSKEFIERTERLYGKGWRPEFDTMDTF
                     VDSSWYYLRYVSARDERVFADTKQLQHWLPVDFYMIGPEHIVLHLLYSRFFTKFLRDQ
                     GYLDFAEPFMKMRHQGMILGPDNKKMSKSKGNVINPDEIIEKFGADTLRTYEMFMGPI
                     EADKPWDVRAVVGVFRFWQRFYNLVFANLESKESLAIEASEPTESQLQVLRKLHKVVQ
                     KITQDFPELKFNTGIASLMELVNTWEEHDAVVLPLEQVGVVIRLVAPLAPFLAEELYQ
                     HYLKVAGTQAAPGFWSVHQAEWPMADPLLAKEDFIVLPVQINGKLRATINLAADNTLT
                     EATLVKQALQLPDVQKWVSEQTLKRTIYVRNKVLNLVM"
     gene            8729..9379
                     /locus_tag="PKH60_01210"
     CDS             8729..9379
                     /locus_tag="PKH60_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNQ16701.1"
                     /translation="MLFRHDLDSKTEWLSLLKRHSKIVVILLIVVGLLISVTDLVWIL
                     MISGKTTVLECPPQTEFVVDASAAAQILVDIRGEIEAPGVYALESTQRLGEAVEKAGG
                     LTKSANAPYVLHALNMAQSLEDGQKIYIPHKDEIEYCRPINQFSSEAQTLVGDEGGLI
                     SINTASASELDTLTGIGAKRAEDIIANRPYVSLEQLTELKIVSNDIFDEIKTQISL"
     gene            9434..10756
                     /locus_tag="PKH60_01215"
     CDS             9434..10756
                     /locus_tag="PKH60_01215"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNQ16702.1"
                     /translation="MRPVVIYTIILAIIILTQLLQLNNPNKYLPNQELAIEKFILKQH
                     SPTCWYVQNQILFKLDGCGRYELGSQLTLLGTLTEPTDSSLSDLFLLEPSAVKVVIKP
                     SELVWYHPKFIQSRLFQIRDNLHHRLRLILPKQDSELYFSLIFGGTSFLPEETKHAIA
                     YLGIQHIMAASGMQVSLLLLLLLPLEKYFSKKLRFLFRFCYLLLYAELALMSVSIVRA
                     VFQSLFSTLFLVKHRQSSYSWSLLLFLLLFLTTEISDSRVALQLTILAVLGLQFRQFL
                     ERLNHSQVGHKNIKQTIQNKAIKYIKEVLITTIWVQVWLFPFLSFYFMQVSSMSLVAT
                     LAISWLLAPLFVLGIILVSVISFLPFSVIQSMPVLLASSPLYFGTWLLRFLIRLMESI
                     ELPPISINFSITALIGWYLCLTLLFWWLDARWFAKIHSDDLKIIRKKY"
     gene            10760..11683
                     /locus_tag="PKH60_01220"
     CDS             10760..11683
                     /locus_tag="PKH60_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ16703.1"
                     /translation="MYKIGLIIVICIIWSIWPHFGFRKDLQIIFCDVGQGDATLVQLG
                     TFQLLVDGGPPNDKVIECLGRYVPFVDRTIELVVATHQDSDHIGGLNSVFANYHVREL
                     VWNGEEKQTADFLTFSQAVQREQDSGMRVSIVAHAGEINIGSNLVATYLFPRVARFSN
                     ATQVSHKCETHLQDITGTNSMTDISSNDGSIVLFVRYKQVTILLMGDQETTGELTLSC
                     LGLLEPMTIVKVGHHGSKSSSSMEFVSRTQPEYAVISVGKNNRFGHPSPEVVSRYDLF
                     RSVLLRTDLMGSIELLSDGDRVWREKSLFDI"
     gene            12256..14148
                     /gene="argS"
                     /locus_tag="PKH60_01225"
     CDS             12256..14148
                     /gene="argS"
                     /locus_tag="PKH60_01225"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNQ16704.1"
                     /translation="MQNAKKQLKDLLLEIVSEFDNFETFLEVEVPTDQSHGDYTSNVS
                     LVLFSKLIKTSTDCQWKKPAELAHYLVKKIQEKKPAWLESVVVAGPGFINFTLTQSCL
                     ASNIGFFLSMSGKTESLINQHQKIIVEFTDPNPFKEFHIGHLYSNTVGESICRLLEAT
                     GAQVCRADYFGDVGMHVAKSIWGMRQLFVEESLDLAQLSQQDLQKRIAFLGRAYAQGA
                     SAYEVDESAQAEMRQLNFLIYKAGQDRLVEQEAWQPVIDFDQFITDEFRKIYPEIKEL
                     FFTGRAWSIEYFDAIYQRLGMKFDYYYPESKAGEWGTRIVIEQLEKGIFEKSQGAVIF
                     AGSQHGLHDRVFLNSLGLPTYEAKELALPKLKKEDFDYDRSIIITANEINEYFKVLLK
                     VLSLIEPEIAAKTTHIGHGVVRLPEGKMSSRTGKIIAGGWLLDEAKTEVIKLASQTKM
                     FSQVDQEEMGELVGQAAIKYAFLKQSIGGNLAFSFSESLDFQGNSGPYLLYSYVRAQN
                     VLRQAAEVSKISIEQLNDLLVNRKIEGTDNYSKLEIDILRNIEKYNDVVEQAAEEFAP
                     HLLCTYLYRLAQTYNTWYADQKIVEVVDGSLADRTIVRLQLNAVVGNILKRGLYLLGI
                     RTVEKM"
     gene            14178..15479
                     /locus_tag="PKH60_01230"
     CDS             14178..15479
                     /locus_tag="PKH60_01230"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNQ16705.1"
                     /translation="MLKHSVQVLDSGLELIRIPMASISSVTALVLGNTGSRYEKPAQQ
                     GLAHFFEHMVFKGTKKYPDPQVLASTIDAVGAQSNAFTSKEYTGYYVKAASKHLERSL
                     DVLSDMLLQPLIRPEDIEREKGVIIEEINMYSDSPMQDIDNVFDQLLFENESLGHDVI
                     GTKETVSGMTHQDFLDLLSGWYGLPNLTLVLAGDATVVESDQTLDLVSKLFGGEKRPQ
                     ETRVDHRVEVAPLLKSSWKNTGRLLVKTKQTEQAHLMLGWPGLVRSDERRYALALLSI
                     VLGGNMSSRLFTEVREKRGLCYYVHSTVDYYHDAGVFGAAAGVDPRRIHEAVQVIVDE
                     FQQLSSAKNAITQQELDRAREFAMGTLVLSLEDSQSVAQYYGVRQLLQRKIDTPDAVI
                     ERLKRVTLEEINLLAQTLLKSGEMRLALIGPFEQAEFEKYV"
     gene            15551..16036
                     /gene="ndk"
                     /locus_tag="PKH60_01235"
     CDS             15551..16036
                     /gene="ndk"
                     /locus_tag="PKH60_01235"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013683162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNQ16706.1"
                     /translation="MSGKTSRTAVLVKPDGLQRGLIGEIISRFERKGLKLIGLKMVSM
                     TDAQLSEWYIEHREKSFFGELKSFMGSLPVVAMVWEGIDAVPVVRKLVGTTLGREAEA
                     GSIRGDFGMSQQFNLIHASANGEDAEREIDILFNIDELFNYSGAMECVVYTDKELEGV
                     R"
     gene            16038..17132
                     /locus_tag="PKH60_01240"
     CDS             16038..17132
                     /locus_tag="PKH60_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16707.1"
                     /translation="MVGGEGVLSQFRPNNELGRNFDVLAARILNELHLDIEKGHFDIA
                     RDLVASIGGVFAQIVPGKSAEIQAALTALIDSQMQQALVEQPQVNSTAEPVVSAEAQA
                     ITTEATVPLKQVSEAEAIPQINACTTIEQVAELIKEWEIPVSLGKLEDGAEVAREPVP
                     GYLAVVRMKTLVDSIIQATDDQLDEVIEKKQKLVPRFGGLREKVIALAKAERTKTIEL
                     PLGPAQDEVVEAEPTLVQKTQEFCRYLSINFGKVYKGDLIDHFVRLLTDVSEGLIPVD
                     PTSATVELDLDEDTIGTSFDTIKLNMLDKAVQSFLTEGDFSTAEQLRACLVTFQIDQK
                     IKMVGTEKDSYWNRRMKYGIAKVVISPKQD"
     gene            complement(17351..17422)
                     /locus_tag="PKH60_01245"
     tRNA            complement(17351..17422)
                     /locus_tag="PKH60_01245"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17387..17389),aa:Arg,seq:ccg)
     gene            complement(17467..17847)
                     /locus_tag="PKH60_01250"
     CDS             complement(17467..17847)
                     /locus_tag="PKH60_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16708.1"
                     /translation="MAKLFLDANCLIDLIENRDETLAQQLTGHQLVISALSIHILCYV
                     GKHTMPSNLLDQALSYFTTVSMNQEIATKSLQGPTPDFEDNVQLHSALFSQCEYFVSR
                     DTKLLQNSACHLAPVIDPAQLQNL"
     gene            complement(17840..18091)
                     /locus_tag="PKH60_01255"
     CDS             complement(17840..18091)
                     /locus_tag="PKH60_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16709.1"
                     /translation="MQFSKTTMNLPTPLLTSAKRYALERNTSVTQLVITGLSQLLATE
                     MSPNRPSLLEFIQQLPAKPKLSPAGRDQLYKATMQSKHG"
     gene            complement(18171..18242)
                     /locus_tag="PKH60_01260"
     tRNA            complement(18171..18242)
                     /locus_tag="PKH60_01260"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18207..18209),aa:Arg,seq:cct)
     gene            18339..18929
                     /locus_tag="PKH60_01265"
     CDS             18339..18929
                     /locus_tag="PKH60_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16710.1"
                     /translation="MKLQQLQTAIQTPLFTSTQVGRLFVTENLDHVRTQLSRWVQRGL
                     LIRHRRGLFQFTDRTVDEMVIAQWLYQPSYVSLESALSIHGLLLEVPQQVTSVTPTTT
                     RRFTTPGGIFRYSKIRTALYFGYEVVTDTNSGLAYNLAQPAKAVLDYVYVRKLQSLDD
                     SRLELDQFARDYSIFSRYLRLYPSWVQTVLGEVCKL"
     gene            18917..19741
                     /locus_tag="PKH60_01270"
     CDS             18917..19741
                     /locus_tag="PKH60_01270"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNQ16711.1"
                     /translation="MQALITQFDQIISFATQMGLPSHKKRGIIREYLQTKFIHDLYAH
                     READQLVFVGGTSLRLLRNIPRFSEDLDFDNLGLRPEQIHVLLENITARWNRENLAVE
                     LVTNRQKAAWYAELRFPRLLSELKISTNPKEKLRIKVDYGNWWRGQKPEQVLCNRFGL
                     IQQVVTNPLNQILVQKLTAYVRRPQTQARDLYDVVWLFAQGARLDQDFTQVNRLKGIG
                     GGVEVDLLTAARTKLASEGVTDVFRRRLQPFLFDEAEVAKIDLFGRVLSELVEIGS"
     gene            19804..20370
                     /locus_tag="PKH60_01275"
     CDS             19804..20370
                     /locus_tag="PKH60_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16712.1"
                     /translation="MNTYTSLAEIEQLLLDKQVRVVTVELLATILSETNLSARQAIIT
                     RLLKKRRIRRLKRGMYELAQTPLTTFEKANVFVSPSYISLESALNYYGILAQFPHVIT
                     SVTPKHSKILNLDQTYEYSQIQPSLFQDFVKDQRFLIASPEKAVADWCYLASKGLRSS
                     DLSQFDLETIDLELLCRNYPWTQSYVKL"
     gene            20357..21142
                     /locus_tag="PKH60_01280"
     CDS             20357..21142
                     /locus_tag="PKH60_01280"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNQ16713.1"
                     /translation="MLSSKALQEFSQQLQIDEFSVLREYVQLAFLNQFYLGKGMELTY
                     FKGGTAIRLVYGSGRFSEDLDFTTNMSQEQIQSAVAITIDKLSVEFPNLSWKNLKSLA
                     GVSLKIYLPVEQAMQPMTIKLDFSLRESVLEPRTSTLSSNYPLALLVLVPHLSAEELL
                     AEKMRALLHREKGRDAYDLLYLLNRKIPMKSDLLQQKFALYDEEFNLQQVKDTIKVLD
                     VKKLHQDLDRFLPKNVRQVVDEIPRLLGEKLSEPSLTRGGGKN"
BASE COUNT         6180 a         3918 c         5122 g         6081 t
ORIGIN      
        1 cggatggtcg tagagttggg tggggtgttg gagtaagagc tactaaatat attgttggtt
       61 taggatgact agtggttgtg ggggcttcca cggtaggaga aactagagtc tctgttaacg
      121 atttgtagat aaactgaatc ggcgtaagcg tgttagtgtg gcccgctgag tcttctatga
      181 tgagagttag agtgactagt gtgttcttat caagactagg aaattgatac gtccaagtac
      241 ctcctgaacc aatgctatat gttgtgctaa cggtgttttg gccagggatt gcaagaatta
      301 taagcaatgt agtatttggt tcaccaagtc cagatataag tggatcctta tgtttggtag
      361 aaaatggttc agttggtaga gtctgactat ttgctgttgt tatcaaaacc gtttcatctt
      421 ctatctttgt aatactcaaa tacggagcta gagtatcaat gctaaaatcc caagtagccg
      481 aagttgctgt attactaaca gcatcagaag ccacgacact ccaagtgtaa tctccgtctg
      541 agatagcttc ttttattgtc agtgagaaag tgttgttgtc ctcatttacc acgagcgtaa
      601 atatggccgt atcttggtca actggtgaga tgttagatat atgggttact tcgtttaacc
      661 aaaagtcgta gtgatcaata ccaacattat ccgtacttcc ataccaaacg aaagtggggg
      721 tgcttgtggt caggatggct tcatcctctg gggatatcag aacggggatg ctgggagatt
      781 caatgtctgg tactgtagca gatactaggg aagctgagct ggtgctaatt tgaacttggg
      841 cctgagcttg gtaaaagtta ctgcagaaga tccagggtat tagaataagc gtgctgacta
      901 cccatttttt ctgagaaaat ctgtcatgca ccgtactttc taatcctggc aaatcagtta
      961 ctacttcgac tccaatcgag acagtttttc ttccagctcc ttgatcttag ctgattgatc
     1021 tgtcttgcga tggattaagt gtctgcgaac ggcgatgaac atggccagta ggacaagcag
     1081 tgtgagtaga gctgctacaa cgagggtgaa ggggaagagc cagaatgaag ttttagcgat
     1141 agcaatcgct ccctgactac cgtaattcat agtaacggtg gcggtgtatc taccagtgcc
     1201 ccagactttc tcccagctac cttcaaatcg gcgttcagtg agcggaaaga tgttcttact
     1261 ctcaattgct acttggtcga cttttctacc cagcatatct ctgaattcga gcattaaacc
     1321 aggttgaata tgaatatcgg attgattctc gatagtaaaa gagtagggta ctggaccata
     1381 ctcagttaac ttgggtatat tgaagcttcg aacaaacgca ctttcattga tagcgccttt
     1441 gacgttgaca tagagtaagg ttcctacccg ttgtgaaata cctgtggcag agagtttggt
     1501 atcggctgtt tcaccatctt tgggtactgc ttcggtactg ggttgatgcg tgatcatcgc
     1561 atagtgaccg ccaggaagag cgtcatctgg aactgcaata atgacggaga tgatctttgt
     1621 ttcattgggt tggatggttt gtagattagg agcaatagtg atccaggaag cgagactcca
     1681 gcggttagag agattctggt ctgttactgc gatgggagta gaaccatcga catcaagcac
     1741 gaaatcatga actgaagaaa gaatagtgag tggtttgtcg gaagtattgc gaactcgaac
     1801 tgtatcttgt aatacttctc caggactggc agtgagagaa ccatcatcac cgagtctggg
     1861 aggaatagcg gtcagtgtag tttgagatgt agtggtagca ggctctaccg gagtctcggt
     1921 ttgggcaagc actactttga caaatgaaaa actcacaaca actgagaaga gaaataaaaa
     1981 cgtggttatg atgcttctga gagtacgctt gggagtgggg cagtgcatag tgtttccttg
     2041 aactacgata ggttagaacg tcgctgtagc ggtatacgtg agatagttgc tgtattcacc
     2101 agctgcctga gtggcagcga tgatagcttt gtagcagacg aagacattgt cgttgtcggc
     2161 gacggtcgta cccgtaaaga tactctgagg tgcttggcta ttttctgcat ccgcaaactg
     2221 cttgaaacag tagttggtgc catcacagtt accagagtta gtgttgtagc tgaaggctgg
     2281 agtgcctgcg cctgcattga cttcatgtaa actatacgca aaacccttag ctgtaccaag
     2341 gctccacttg gcgggcgcgg tatgcgacat agcgccgcca tcaccaactg agtcgcggat
     2401 acagttaacc cccgtattgt ctccagtaca gacaccaccg ttacgaccaa gctgatcatt
     2461 ttctacggtg gtaaccgcat agccattggg tgcattggta gagacagaaa gtgcctgggc
     2521 ggcagtggta aaggctgaga tgctcagtga ccccaagggt acaaggctgg cagtagttga
     2581 tacatcagta ttcacgccac acacggaggt gccactattg actcccaaaa tgcggaaggt
     2641 gatttgaggt gagacagtag cagttactct tactgactca actacgccaa cattaaccgt
     2701 tgtagcgtca gccacgttat atgtactatc tacctggcgg acgatgatgt tgtaggtgtc
     2761 ggcataaccg atggtgtggc ctgtacgcga aggggctgga ttaatcaggg aagtaatggt
     2821 aaatgctcca tatgttgtgc catcaaacac tgtgccaatg gctcccgtgc catagtatcg
     2881 acactcaaat gagtgatact tggtgccatt gacggtgact gcggaggcgg tggcagttcc
     2941 agttgcaaaa agataggtgg cgggagcact agatggacag gtaacggtgg gagcactggt
     3001 gccaaagtca aagccagtac catcggggat accatctctg gccgctgcgt cagtggagtc
     3061 tgcaggaact aaaatgcgga aagcaccatt ggcgatggca ttcgcagtag tgagtcgaac
     3121 ggtgagcgta gctgaagtag aggctacgac atcgtcattc aattcggtgt cgccggatgc
     3181 caaaactggc gtaacattta ctgtactatt gtctacgatt tcagtaatag tatatgtccc
     3241 caaacttcca gcctcaccga ttctgagtga gtcaccaacc actagttgag ccgaagatga
     3301 ggatggccag acaccattgg ttgtattcag gattacttct gaggaaccga cggtattgcc
     3361 agaagccagt ccacctctga aagacggtct ggagctggaa agcgtaacgg atacattaga
     3421 aagattagct gagcgagtgc cctggagatg accaaccaca aacatcccaa gcaccaaaca
     3481 agtagccaaa ccaatgcgtt gtacgcttga aacagagagg attttcataa attcttcacc
     3541 agaatatatt ctatgtattc agtacatcat aaaaactcaa gtgatgcaag agttagttta
     3601 gtctggggat gtattagtag caaacactcg agaaaatacc catccaataa tgataaacaa
     3661 tactacaatt aaaaacgagt agggaagcgc gattacagtt tttgtgtgcg aactactact
     3721 atttccttga ccatcaatta ttttggtggt aatggtatat tttcctagca gaaacagtgg
     3781 gtcatatgaa aaaacagtag gttctggaag tttactgtct ttttgtgtcg tgagagctcg
     3841 agcggaacgt tgttgtctgc ccaaaatcat gtcgggatag atttgtgcct gaaatacggt
     3901 tctgccaaag gtgtcgacaa tcgtggcact accactggca acaacagttt gtaccccttg
     3961 gttaataagc attggagtaa atgaaagccc gcgtaaacta tcaagaagcc ttggcacttc
     4021 aatgtctact acttgcagta agttgggcag agtttcgtcg ctactcacca gaactacgag
     4081 attagcgcca atcactccat acagtttgga atttgaaact acttgattag ttgtatttgc
     4141 ttgttcaata gttgatgaaa acagcacagt aaggggatat tctctagtaa ttgcttcggg
     4201 ggggaccgta aaatctaaag aaaaactatt tgtttctcct ggctttatca aaattggcga
     4261 cattttttgt acttgttccg acagataagg aaacgaaaat gttggttgca tttccgggta
     4321 acctgagtct tcttccggtt tagaatcaaa cacctttgga gtaaccacaa ttgtattagt
     4381 tgtggtattg gtcaattcta tggtgtggtt tgccgtggtt cctggtttga tttttagata
     4441 agcactcggg gggtaaactg acaaagaata atcagattgt gctgaaatgg atgtggggta
     4501 ttgcagccaa aaccaactaa ttccgcaaaa aaccactact aaatgccatg ttttaatttt
     4561 gttactagta atcatagtgt atttattagt gttattaata tgctggtact gcgacaaata
     4621 cgatagaagt ttcataggta cctgcaggtt cactaccaaa tagacccgct ttataggtga
     4681 ctgttgcagc gcgattaacg acatatccag tattgctcat aatgacctgc atcggttcac
     4741 tcaaggcatt gttggcaaac tgacgaaagt aatttgtatt aataaagtca gccgctgtgt
     4801 cgtcaccatt aacattgaaa ccaaatccgc caacatcagt attttgccag atctctgcgg
     4861 tggtttggtc acagtcatta ttgtcgcaag ttgtatcagg aatagtgggt atgccattta
     4921 gcagttttaa cggatgttgt tcatatgcat aaatctggta accaccagct gttggtgcgg
     4981 taatggtgag gacattagtg tcggtattat gcgcttgtgc cgttaattga cccaaattaa
     5041 tgtctgtttt tgaaattgtg aagcgaaatt gatcgattgg ataaatgtac tgaaaacctc
     5101 caccaacaaa ataacttgcg gtgccaaatt caccataggg acctgcgcct gtttggccga
     5161 ctgtatcggt tacgttgtag gttgtactag atttttctcc agaagtaaca ttaaaattgc
     5221 caaattgaat tataaatgag ttcgactcaa gtgtttctgc ataaactgcc tggctggata
     5281 agcaaatcaa aatcagcact agtaaggttc caaaaaaacc atacaagacc atttgaggat
     5341 gcgtcgggtt agacaagttc attttcagtt ttttcctcta cacttggttg gagaagtatg
     5401 gcttggtcga tttgctgatt gaggtcatca gcttcagcca aagcctttgg ttcacaagca
     5461 aaatattgct gtttccattg agtgatttgt tgacgagctt ggtctagtag agcacggcct
     5521 tcaattagtt gagagcgttg attgttttta tctttttcta gttttgccaa cttatcttgt
     5581 agttttctgg tttcttttga gcactgcgta agtaaaacct cctcctgagc tatggtttgt
     5641 ttttctttaa ttacttcttc atgttgttct ctggcaaata gaaaaaatag caaaataatt
     5701 gggatggtga tcaggtgtcc aatgtttgag agagaaatag ttggttctag tccaaaatga
     5761 aagagcgcgg tagctggacc aacggtgatt gctgtgcttc ttgatgaact catgactaaa
     5821 aaaaagaggt gaatataggt caggggaaag aggggtgagt ttaaagtacc actgttacct
     5881 actagcagta agaggctaaa agtgactagc gccatttcgg ctgaattttc ttgtggcagg
     5941 atgtgtttaa gttttgctcg agtatatttt ttgattacaa agtaacaaat ggttgatgcg
     6001 gcaacagctt gaaggctcag agatttaagc atgggaactt gcaaccagac gaatgccaaa
     6061 aaaatggcaa acagcaaaag ggcagtgtgc tgtcggggat gcatacttct atactactat
     6121 aaggtaaaac gaaactctat ggagatttat gctatactcg agagcattgt gaaaaaacca
     6181 ataccccaat cgtacaaccc ctccgaatct gaaaaacatt ggctatctga atgggagaaa
     6241 ataggcttat ttcaatcgac tacgaaggag ctatccgaaa aagaaaagtt ttatttgctg
     6301 tttgcctttg cgtatccctc gggatctggt ctgcatgtgg gtcatgtgga aagcatgact
     6361 gctcttgata ttttggctcg tttcaaccga atgcaaggca agcacgtttt ttttccagtg
     6421 gggtgggatg catttggttt gccagcggaa aattatgcga ttaaaacagg agtagctccg
     6481 gcaattacga cgaagcaagc aatcgatacg tttcatgaac aaattaagag gttggcgatt
     6541 tcgtatgact ggtcgagcga aatcgctacg tgtcaccctg gatactacaa gtggacacaa
     6601 tggttgtttt tgcaacttca taagcagagt ttagcctatc aaggtactgg aacagtaaat
     6661 tggtgtcctt cgtgtcaaac ggtgttagcg aatgagcaag ttgtggatgg tttgtgcgaa
     6721 cgatgcggag ctgctgtcgt gcaaaaacaa atgaaacagt ggtatttcaa gataaccgag
     6781 tatcaagacg agttaatctc gggattggaa gaagttgatt ggcctcatgc gactaaacaa
     6841 cagcaattga attggattgg taaaaaaact ggtattacta ttacctatca ggtacaaaac
     6901 cagtcagggg aaaacgctgg cgaaattagt tgtttcacta ccaggccgga tactaatttt
     6961 ggagccacgt ttgtggtaat tgccccagag catgcattgg ctcaagcatt agcaaaaact
     7021 tcacctgaaa tcgcagaata tgttgagaaa acgcttcata agactgagct tgagagacag
     7081 caagagggaa gagtgaaaac tggtgtagat accggactga tggcagttaa tcaattaacc
     7141 ggtcgaaaac tacccatttg ggtctctgac tttgtattgg caggatttgg gacaggagca
     7201 gtagttggag tgccaggtca tgataaaaga gattttgagt ttgcgcgaac tttttcactc
     7261 ccaattttaa gagtagtgaa agcagccgat ggtgacatct cagaaattac caagctagaa
     7321 caagttcaag aatcggaagg agtgatggta aactctggat ttttagatgg catggcaatt
     7381 tctgaggcta ttgaagcaat gacggcacat ctggaaaagc agggttgggg caaacgagtg
     7441 acaacctaca agcttagaga ttggctgatc agtcgccagc gctattgggg tgcgccgatt
     7501 ccaattgttt atgatccaga gggccaacct catccagtca aagaagagca cctgccttgg
     7561 ttgttaccaa ctgacgttga ttttaaacct accggagaat cacccctgca atcttccaaa
     7621 gaatttattg agagaacgga gcgattgtat ggcaagggtt ggagacccga atttgacaca
     7681 atggatacct ttgttgactc aagttggtac tacctcagat acgtctcggc gagagatgag
     7741 agggtttttg cagacactaa gcagttgcaa cactggttgc cggtagattt ttatatgatt
     7801 ggaccagaac acattgtgtt acatctgctt tactcaaggt tttttacaaa atttttacga
     7861 gatcagggat atcttgattt tgcggaacca tttatgaaga tgcgccatca gggcatgatc
     7921 ttgggtccag acaacaaaaa aatgagcaag agcaagggca atgtaattaa tccagatgaa
     7981 atcatcgaaa aatttggtgc agatacctta cgaacgtatg aaatgtttat ggggccaatc
     8041 gaggctgaca agccgtggga tgtccgagcg gtggttggag tctttcggtt ttggcagcgc
     8101 ttttataatt tagtatttgc caatttggag tctaaagaat cactggctat agaggcatct
     8161 gagccaacag agtcgcagtt acaagtacta cgcaagttac acaaagtagt acaaaaaatt
     8221 actcaggatt ttcctgaatt gaaatttaat acaggcatag cttcgttgat ggagctggtg
     8281 aatacttggg aagagcatga tgctgtagtt ttgccattgg agcaagttgg tgtggtgatt
     8341 cgattggtgg cgccgctagc tccattcttg gctgaggaac tctatcagca ctatcttaag
     8401 gttgctggta ctcaagcggc gcctgggttc tggtccgttc atcaggctga atggcctatg
     8461 gcagatccac tgttggcaaa agaagatttc attgttttgc cagttcaaat taacggaaaa
     8521 ttacgtgcca ccattaacct ggctgctgat aatactctaa cggaggcaac cctggttaag
     8581 caagctttac agctcccaga tgtgcaaaaa tgggtatcag agcagacttt aaaacgaacg
     8641 atctatgttc gcaacaaggt tctgaattta gttatgtagc ctcaaatacg ctacggttgt
     8701 tgttagatac tattgataga ttagggttgt gctattcaga catgacttag attcaaaaac
     8761 cgaatggtta tcgttgctca aacgccacag taaaatagta gtaattttgc taatagtagt
     8821 ggggttgttg attagcgtga ctgatttagt ctggatcctc atgattagtg gcaagaccac
     8881 cgtgctagag tgtcctccgc aaaccgagtt tgtagttgat gcaagtgcag cggctcaaat
     8941 actggttgat attcgaggag agatagaagc tccaggtgtc tatgcccttg aatcaacaca
     9001 acgtttggga gaagcagttg agaaagctgg tggactaact aagtcagcca atgcgccata
     9061 tgttttgcat gcactcaata tggcgcaatc cctagaggac ggacaaaaaa tctacattcc
     9121 acacaaagat gagattgagt attgtcgtcc aattaaccaa tttagttctg aggcacaaac
     9181 cttagttggt gatgagggtg gtctaatttc aatcaataca gcgagcgcaa gcgagttaga
     9241 taccctaact ggtattggtg caaaaagagc cgaagacata attgcaaacc gcccgtatgt
     9301 ttcacttgaa caattaacag agctaaaaat agtgtcaaat gacatatttg atgagattaa
     9361 aactcaaatt agtttataaa attattaagt ctactaaatt aataaataag tattccgctc
     9421 ctataacgta attatgcgtc cagtagtaat atatacgatc atcttagcaa taataatact
     9481 cacgcaatta ctgcaattaa ataatccaaa caaatattta ccgaatcagg agctcgcaat
     9541 tgaaaaattt atacttaaac agcactctcc gacctgttgg tatgttcaga accaaattct
     9601 ttttaagtta gatggttgtg gaaggtatga gcttggcagt caactaaccc tacttggcac
     9661 tctcaccgag ccgactgata gctcgttaag cgatctcttt ttacttgaac catcggctgt
     9721 aaaggtcgta attaaaccat cggaactagt ttggtatcat ccgaagttta tccaatccag
     9781 attgttccag attagagata atctacatca tcgattgcgt ctaatcctac caaaacaaga
     9841 ctccgagctg tatttttcac ttatatttgg agggacctca tttctaccag aagaaacaaa
     9901 acatgctata gcgtatttag gaattcaaca cattatggct gcatctggaa tgcaggtaag
     9961 tctgctattg ctgttattac tacccctaga aaagtacttt tccaaaaagc ttagatttct
    10021 atttcgattt tgctacttat tgctatatgc tgagctggcc ttgatgagtg tatcgattgt
    10081 aagagcggtg ttccagagct tattcagcac tttatttttg gtaaaacatc ggcaaagctc
    10141 ctattcgtgg tcactactgt tatttttatt acttttcctt acaactgaaa tttcagacag
    10201 tcgagtggca ttgcaattaa ccatcctagc agtcttaggc ttgcaattcc ggcagttttt
    10261 agaaagactg aatcattccc aggttgggca taagaacata aaacaaacga tacaaaataa
    10321 agcaataaag tatataaaag aagtacttat aacaacgata tgggtgcaag tttggctgtt
    10381 tccttttctc agtttttatt ttatgcaagt gagtagcatg agtcttgttg ctactctagc
    10441 tatttcttgg ttgctagccc ctctatttgt gctcggaatt attttggtta gtgtaatttc
    10501 ttttttacct ttttctgtta ttcagtcgat gccagtgttg ctagcttcct caccgttgta
    10561 ctttggaact tggttgttgc gctttcttat ccgtttgatg gaatcaatag agcttccacc
    10621 aatctcaatt aatttttcaa taactgcatt gattggttgg tatctttgct tgactctact
    10681 attctggtgg ttagatgcgc gttggtttgc aaaaattcac agtgatgatc taaaaattat
    10741 ccgaaagaaa tactaagtaa tgtataagat aggtttaatt atagttattt gcattatctg
    10801 gagtatttgg ccacactttg ggtttcggaa agatttgcaa attatttttt gcgatgtggg
    10861 gcagggtgat gcaaccttgg ttcagctggg aacttttcag ttactggttg atggtggacc
    10921 gcccaatgac aaggttattg agtgtttggg gaggtatgtg ccttttgttg atcgtacaat
    10981 tgagttggtg gtcgccactc accaagactc cgatcatatt ggcggtttaa actcagtttt
    11041 tgccaattat cacgtacgtg agcttgtgtg gaatggagaa gaaaaacaaa ctgccgattt
    11101 tttaaccttt agtcaagccg tccagagaga gcaagatagt ggaatgcgtg tttcaatagt
    11161 ggcccacgct ggtgaaataa acattggctc aaatctagta gcgacgtatc tatttcctcg
    11221 agtagcaaga ttctcaaatg caacccaagt aagtcacaaa tgcgaaacac acttacagga
    11281 cataactggt acaaacagta tgactgatat cagttctaat gacggatcta tcgttctatt
    11341 tgttaggtat aaacaagtaa caattttgtt gatgggggac caagaaacca ctggtgaatt
    11401 aacactaagt tgcttgggtt tgctagagcc aatgactata gtaaaagtcg ggcaccatgg
    11461 atcaaaatca agttctagta tggaatttgt gagtcggacg caacccgaat atgcggtcat
    11521 tagtgtgggt aaaaataacc gatttgggca cccatcacca gaggtagtga gtcgttatga
    11581 tctatttaga tcagtgctcc ttcgtaccga cttaatgggt tctattgagt tgttaagcga
    11641 cggagatcga gtgtggagag aaaaatctct ctttgatatc taaatccgtg agcaccactc
    11701 tcacatgaga tcactatttt gtcaacgagc agtttaatga tattgggacg tgaccagatc
    11761 atagactaga attaatttat atcctatata agatttatcg ttttaattaa atactaagag
    11821 taaatatagc tgtgcattct atctggaagt tgaaatggaa gcaaaaattg ttcagatctg
    11881 attacatagt agcccagatt agtttattga tttctgagag agacaaaaaa attcaatcat
    11941 aaaataaaca tgctctgcag aagatagtgg ggatcagtgc tttctgcaaa aataaatcca
    12001 cagtaaactc tacgcggtag gattcggtat tacccgaaac tatttttcta ctgaagcaaa
    12061 gaggctgaat gcaatttgaa gaaatttgaa aaatactaat aaaagagtaa taaatgacgc
    12121 cataaataaa gaaaatattt actaacctag caacccacga agaatacaaa tagtactcaa
    12181 aaacttcggt aattgttcgc tataacacga ttgcgcattc tgctacaatg gtagaataaa
    12241 acaaggacgt tgcagatgca aaatgccaag aaacagctca aagatcttct gctagaaata
    12301 gtaagtgagt ttgacaattt cgaaactttt ttggaggtcg aagttcccac cgaccaatcg
    12361 catggtgatt acacatcaaa tgtgtcgcta gtgctattta gtaaactgat aaaaacgagt
    12421 acagactgtc agtggaaaaa gcccgctgaa ttggcccatt atttagtgaa gaaaattcag
    12481 gaaaagaaac cagcctggct tgaatctgta gttgtagcag gccctggttt tattaacttc
    12541 accttgactc agagctgtct agcctccaat atcggctttt tcttgtctat gagtggcaag
    12601 actgagtcgc taattaatca gcaccaaaaa atcattgtgg aattcaccga ccccaatcca
    12661 tttaaggaat tccacattgg gcatttatat agcaacacgg tgggcgaatc gatctgccgc
    12721 ttgttggaag cgaccggcgc acaagtctgt cgggcagatt actttggcga tgttggtatg
    12781 cacgttgcca agagtatctg gggtatgcgc cagttgtttg tagaggagtc acttgatttg
    12841 gctcagcttt ctcagcaaga tcttcagaag cgaatcgcgt ttttgggacg ggcatatgcc
    12901 caaggagcct ctgcctacga agtcgatgaa tctgcccagg cagagatgcg tcaattgaac
    12961 tttttaattt acaaagctgg tcaagatcgc ttggttgaac aagaagcatg gcaaccagtg
    13021 atagattttg accaatttat tactgatgaa ttcagaaaaa tctatccaga gattaaggag
    13081 ctatttttta ctggcagagc ttggagtata gagtattttg atgcaatcta tcagcggttg
    13141 ggaatgaaat ttgactacta ttatcctgag agcaaagcgg gcgagtgggg tacaagaatt
    13201 gtaatagagc aactggaaaa aggaattttt gaaaaaagcc agggagcggt tatttttgct
    13261 ggcagtcagc acggcctgca cgatcgagtc tttctcaatt cacttggatt gccgacctat
    13321 gaagccaagg agctggcgct accaaaactt aaaaaggagg attttgatta tgatcgctca
    13381 ataataataa cggcaaatga aattaacgaa tattttaaag ttttactcaa ggttttgtcg
    13441 ttaattgagc cagaaatagc agccaaaaca acccatattg gccacggtgt tgtcaggctt
    13501 cctgagggta aaatgtccag tcgaaccggt aagattattg ctggaggttg gttgctcgac
    13561 gaagccaaaa ctgaagtaat caagttggct tctcaaacaa aaatgttttc tcaggttgat
    13621 caagaggaga tgggagaatt agttggtcaa gccgccatta agtacgcttt cttgaagcaa
    13681 tcaattggag gtaacttagc gtttagtttt agcgaatcat tagattttca aggcaattct
    13741 ggaccatatt tattgtattc ctatgtgagg gcccaaaacg tgttgcgaca agcagccgaa
    13801 gtatcaaaaa tatctataga gcaacttaat gatctattag tgaatagaaa gatagagggc
    13861 acagataatt actcaaagtt agagattgat atattaagaa atatagagaa atataatgat
    13921 gttgttgagc aagcagcaga agaatttgcc ccacatttac tttgcacgta cttgtatcgt
    13981 ttggcacaaa catataacac ttggtatgca gatcagaaaa ttgttgaagt agtggatgga
    14041 agcctcgctg atagaacgat tgttcgatta caactgaatg cagtcgttgg caacatctta
    14101 aaaagaggtc tctatctcct cggaattaga actgtagaaa aaatgtaacg tgattaatta
    14161 ttcgaaaggt ggattgtatg ttaaaacact cggttcaagt actggattct ggtctcgaat
    14221 tgatcagaat cccgatggca tcgatttcat cagtcactgc gttggtttta ggcaacacag
    14281 gttctcgcta tgaaaaacca gcgcagcagg gcttggcaca tttttttgaa catatggttt
    14341 ttaaggggac aaaaaaatat cctgatccgc aagtgttagc gagtactatt gacgccgttg
    14401 gggcgcagtc caacgctttt accagcaaag aatacaccgg ctattacgtt aaggcagcca
    14461 gcaagcattt ggagcgatcg ttggatgtgc tttcagatat gcttttacag ccactgatta
    14521 gaccagagga tattgagcga gaaaaaggtg tgattattga agagatcaat atgtactccg
    14581 attcgccaat gcaggatatt gataatgtgt ttgatcaatt gttgtttgaa aatgagagcc
    14641 tgggacacga cgtgatcggc acgaaagaaa ctgtgagtgg gatgactcat caagactttc
    14701 ttgatctatt gtctggttgg tatggtttgc caaatcttac gttagtgctt gctggtgacg
    14761 ctacagtagt ggagtcagat caaactctag acttagtcag taagcttttt ggtggagaaa
    14821 agcgcccaca agaaactcgc gtagatcatc gtgttgaggt agcgccgcta ctcaaatcca
    14881 gctggaaaaa tacgggaaga ctattggtca aaacgaagca aacggagcaa gctcatttga
    14941 tgttgggttg gccaggtttg gtccggagtg atgaacggcg gtatgcgctc gctctactgt
    15001 caatcgtttt gggcggcaac atgagctcac ggttgtttac tgaggtgcga gagaagcgtg
    15061 gattgtgtta ctacgtgcac tcgacggtgg attattatca tgatgctggg gtatttggcg
    15121 ccgcggcagg cgttgacccg cggcgcatcc acgaagcggt acaggtcatc gtggatgagt
    15181 ttcaacaatt aagttctgct aaaaacgcta ttactcagca agaacttgat cgagctcgcg
    15241 aatttgccat gggcacttta gtcctatctt tggaagatag ccaaagtgtg gcgcagtact
    15301 atggggttcg ccagttgttg cagagaaaaa ttgatacgcc agacgcagtt attgaaagat
    15361 tgaagagagt gacgttggaa gaaatcaatc tgttagccca gacgctgctt aagtctggtg
    15421 agatgcgcct agcgctgatt ggtccatttg aacaggccga atttgaaaaa tacgtgtaag
    15481 tggttcagtc tggtatacta acttgactac acaattgtat ctgtagaatt attttgatta
    15541 aggagatgtt atgtccggaa aaactagtcg taccgcagta ctcgtgaagc cagatggtct
    15601 gcaacgcggg ctaattggcg aaatcattag tcgttttgaa cgcaagggtt tgaagttaat
    15661 tggtttgaaa atggtcagta tgactgatgc tcaactatcc gaatggtaca ttgagcaccg
    15721 cgaaaagagt ttctttggcg agctcaagag tttcatgggg agtttgccag tggtggcgat
    15781 ggtttgggaa ggcattgatg cagtgccagt ggtgcgcaag ttggtaggta ccacgctggg
    15841 cagagaagcc gaggctggct ccattcgggg tgactttggc atgagccaac aatttaacct
    15901 gattcacgct tcagccaacg gtgaagatgc tgagcgtgaa attgacattc tctttaacat
    15961 tgacgagctc tttaattact ctggtgccat ggagtgtgtg gtctacacgg acaaggagct
    16021 ggagggggtg aggtaggatg gtgggagggg aaggagtttt aagtcaattc agaccgaata
    16081 atgaacttgg tcgtaatttt gatgtattag cagcaagaat tcttaatgaa ttgcacttgg
    16141 atatagagaa aggtcatttt gatattgcgc gagatttggt tgcaagtatt gggggtgttt
    16201 ttgcgcaaat tgtccctggt aaatcagcgg aaattcaagc ggcgctaacc gcgcttattg
    16261 attcgcaaat gcaacaagcg cttgtggagc aaccccaggt caatagcacg gctgagcctg
    16321 tagtttcggc tgaggctcaa gcaataacaa cggaagcgac tgtaccactc aagcaagtaa
    16381 gtgaagcaga agcgatcccg caaattaacg cctgcacaac aattgagcaa gtggcagagc
    16441 tgattaaaga atgggaaatt ccagttagtt taggtaaatt agaagatggt gctgaagttg
    16501 ctcgtgagcc tgttcctggt tatttagcag ttgttagaat gaaaactctc gtcgactcaa
    16561 tcattcaagc gactgatgat cagcttgatg aggttatcga gaaaaaacaa aaattggttc
    16621 ctcggttcgg tggcttgaga gaaaaagtga ttgctcttgc taaagctgag cggacaaaaa
    16681 cgatagagtt accgctgggt cctgcgcaag atgaagtcgt ggaagcagaa ccaacactcg
    16741 ttcaaaaaac tcaagaattc tgtcgttatt tgagtataaa ctttggaaaa gtatataagg
    16801 gagatcttat agatcatttt gtcagacttt tgaccgatgt gagcgaagga ctaatacctg
    16861 ttgatcccac atctgcaacg gtggagctgg atcttgacga agatactatt ggtacgagtt
    16921 tcgacacaat taagctaaac atgttagata aagccgtgca gtcgtttttg acagaaggtg
    16981 acttctcaac agcggaacaa ttgcgggctt gtttggtcac ttttcagata gaccagaaga
    17041 tcaagatggt tggaactgag aaagactcat attggaacag aagaatgaaa tatgggattg
    17101 caaaagtggt gatttcacca aagcaagatt gatcatttat ttcctgcact cttgtatttc
    17161 aattggttgt ttacactaaa gcataacccc ctctggagcc gtgctttact gtaaaccatt
    17221 gttttattca aggagtgttg atagggccaa tgtctacttc gacgggctta gtatgacatt
    17281 ggtctgtcac gggcttagca tgacattaac ctgctgcact gaagttactt tttgtttgcg
    17341 gtcgaagtag tgcccccagt ggggatcgaa cccacattaa aggttccgga aaccttcgtt
    17401 ctatccatta aactatggga gcggatagtt ggcgattgta ttgtaactca gtaccaatag
    17461 cttcaatcac aggttttgta attgagctgg gtcgattaca ggagcgagat gacaggcaga
    17521 gttctgaagc aattttgtat ctctggaaac aaaatactcg cattgagaaa aaagagcaga
    17581 atgaagttgc acgttgtctt caaaatcagg agtaggtcct tgtaagcttt ttgtagctat
    17641 ctcttgattc atgctgactg tggtgaagta gctgagtgct tggtcaagca ggtttgaagg
    17701 catggtatgt ttgccaacgt agcagaggat atgaatcgat aacgcagaga taaccaactg
    17761 atggcccgtg agttgttgcg cgagtgtttc atctcggttc tcgatgaggt cgattagaca
    17821 gttggcatca agaaacagtt tagccatgtt tgctttgcat tgtagctttg tacagttgat
    17881 cgcgtccagc tggactcaac tttggttttg ctggtaattg ttgaatgaat tctaataaac
    17941 ttggtcggtt gggtgacatt tctgttgcca acaattgact taagccggtg atgactagtt
    18001 gagtgaccga tgtgtttcgt tcaagtgcgt agcgcttagc agaggttaat aacggagtag
    18061 gtaaattcat agttgttttt gaaaattgca tgaatacata ctaccatatg tatgtattag
    18121 actcaagcag tatctaatgg tagagcaact agtggtaatt aagtgtggtg tgcccccacc
    18181 aggactcgaa cctggatcag aggtttagga aacctccgtt ctatcccttg aactatggga
    18241 gcgctactcg gtattgtagc agaaaacagg ataagctagg atgatggacc atcgattact
    18301 gtttacatta aatgtacaat aatgtatagt atgtgtaaat gaaactacaa cagttacaaa
    18361 ctgctattca aaccccgtta tttacaagca ctcaggtggg acgcctattt gttacagaaa
    18421 atctagatca tgttcgaaca caactgtctc gatgggtcca gcgggggttg ctgattcgtc
    18481 accgacgagg gttgtttcag tttaccgatc gaaccgtcga tgaaatggtt attgctcaat
    18541 ggctgtacca gccttcttat gtcagcctag aatcagctct tagcattcat ggattacttt
    18601 tagaagtgcc tcagcaagtt acgagtgtca cgccgactac taccagacgg tttacgactc
    18661 cgggtgggat atttaggtat agcaaaattc gtacggcgct gtattttggt tatgaggtgg
    18721 tgaccgatac taacagtggt ttggcgtata atttggcgca accagccaaa gcagtgttgg
    18781 attatgtgta tgtgcgaaaa ctacagtcgt tggatgatag tcgccttgag ctcgatcaat
    18841 tcgctagaga ttacagtata ttcagtcgct atctgcggtt gtacccatcc tgggtacaga
    18901 cagtgttagg agaagtatgc aagctctaat tactcaattt gaccaaatta ttagttttgc
    18961 aacacagatg ggattgccga gccacaaaaa gcgaggcatc attcgggagt acttacaaac
    19021 taagtttatt cacgacctat acgctcacag ggaagctgat cagctagtat ttgtgggagg
    19081 taccagcttg cggttattgc gaaatattcc ccgtttctcc gaagatttag attttgacaa
    19141 tttgggttta cgaccagagc aaattcacgt tctactagag aatataacag cccgttggaa
    19201 tcgagagaac ttggctgtgg agctggtgac taatcgtcag aaggctgctt ggtacgcaga
    19261 attgcgtttc ccccgcttgt tgtctgaatt gaaaatttca acaaatccaa aagaaaagct
    19321 acgaataaaa gtggattatg ggaattggtg gcgaggtcaa aagccagagc aagtgttgtg
    19381 caatcgcttt ggtttgattc agcaggtagt tacgaatcct cttaatcaaa tactcgtaca
    19441 aaaactgaca gcttacgtgc gtcgccccca gacccaagct cgcgacttat atgatgtggt
    19501 gtggttattt gctcaaggag cgcgattaga tcaagatttt acgcaggtta atcggttgaa
    19561 gggtattggt ggtggggtag aagtggatct gttaactgcg gctcggacga aattagcaag
    19621 cgaaggtgtg actgatgtat ttcgacgtcg tctccaacct ttcttatttg acgaagcaga
    19681 agtggccaaa attgatctgt ttggacgagt attgagtgag ttggtcgaga taggaagtta
    19741 gagttgtgaa atcagaacca agtcttgaca cgtgtgcagt tatagtataa atttgtagta
    19801 taaatgaaca cgtatacgag tttagcagaa atagagcagc tacttcttga taagcaagtt
    19861 agagtggtga cggtggagtt gttagccact atcctgtcag aaaccaactt gtcggctcga
    19921 caggctatta ttactcgttt gttgaagaaa cgtcggatca ggagattaaa gcggggcatg
    19981 tatgaattag cgcaaacacc tctaactacc tttgagaagg ccaatgtatt tgtatctcct
    20041 tcatatatca gtctagaatc agctcttaac tactacggca ttttagccca atttcctcac
    20101 gtaattacct cggtgacgcc aaaacattcc aagattttga acctagacca gacctatgag
    20161 tactcgcaga tccaacccag tttgtttcaa gactttgtta aagaccaacg attcttgatt
    20221 gctagtccag aaaaagcggt agcagattgg tgttacttgg ctagtaaggg attgcgatca
    20281 agcgatttaa gtcagtttga tctagaaact attgatcttg agttgctttg tagaaattat
    20341 ccatggactc agtcatatgt taagctctaa agctcttcaa gaatttagtc agcagctaca
    20401 aatcgatgaa ttttcggtgt tacgcgaata tgtacagctg gcttttttga accagtttta
    20461 tttgggcaag ggtatggaac tgacgtattt taagggcggg acggcgatta gattggttta
    20521 tgggtcaggt cgtttttcag aagatttgga ttttactacc aacatgtctc aagaacagat
    20581 tcaatctgca gttgcgataa caattgataa gctgagtgtg gaatttccca atttgagttg
    20641 gaaaaatctc aaatcattgg cgggagtgag tttgaaaatc tatttacctg tggaacaggc
    20701 gatgcagccg atgacaatta agctggattt ttctttacga gagtcggtgc ttgagcctcg
    20761 aacgtcaaca ttgagtagta attatccatt ggctctgttg gttttagtgc cacacctgtc
    20821 agcagaagag ttattggcag aaaaaatgcg agcacttttg catcgagaga aggggcgaga
    20881 tgcctatgat ttgttatatt tgttaaacag aaaaataccg atgaaaagcg acttactaca
    20941 acagaaattt gctttatatg atgaggagtt taacctgcag caggttaagg atacgattaa
    21001 ggtattagat gttaaaaaac tccatcaaga tctggaccgt ttcctaccaa aaaatgtcag
    21061 acaggtggta gatgagattc cccggttgct gggtgagaaa ttgtccgaac ccagcctcac
    21121 cagaggaggg ggaaaaaact gactagtttg cagtagttac tttacaagat ctaatcaccg
    21181 aatagacacc gaagtagaaa gatactcggt tttctgttag tgaaaaaaat attttccaga
    21241 ttgggattaa aaagttagaa aataatggaa aattgtgtct attttgtaga caaatagtaa
    21301 t
//
LOCUS       DAOQZP010000011        21258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_2847_length_21258_cov_5.464793, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000011 DAOQZP010000000
VERSION     DAOQZP010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 21258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21258
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2847_length_21258_cov_5.464793"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /gene="grpE"
                     /locus_tag="PKH60_01285"
     CDS             <1..855
                     /gene="grpE"
                     /locus_tag="PKH60_01285"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNQ16714.1"
                     /translation="KTTVSHVTHDGQAVAGIGKFMVGVGAVIELGTSGTFLVMKRQDE
                     FHHGAWEIVYGRLDQHEDLVTGLKREVREETGISQLEVLDHYSQWHFYRGKKTAETEV
                     IGISFSCRTNQEEIKLSTEHSEYAWVTAEEMLKLSTVPDIHADMKKYLEYRDRQAKLE
                     QSQAVATRALADYQNLVRRHQDDRQKFAQLANLDFITSLLEPLEHLQLASAQLNDKGL
                     QMIVAQLVERLKAQGLEPINPIGLPFDVTTMEAVDSKGEGKIVENVVKIGWKLNDHVI
                     QHAKVGLV"
     gene            941..2902
                     /gene="dnaK"
                     /locus_tag="PKH60_01290"
     CDS             941..2902
                     /gene="dnaK"
                     /locus_tag="PKH60_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNQ16715.1"
                     /translation="MASSGKIIGIDLGTTNSAVAVMEGGSPKVIPSAEGRNTFPSIVS
                     FKGSDVSVGDIAKRQMVLNPKKTVNSVKRFMGRKLNDGAVKAALKHVAYSIVEGKDGM
                     AMVEIDDKKYSPQEISAKILAKAKHDAESYLGGTVDRAVITVPAYFDDSQRQATKQAG
                     EIAGLTVERIVNEPTAAALAYGLDKKGSKTIAVYDLGGGTFDISILEIGDGVFEVKST
                     NGDTFLGGDDFDNIIIEWIVEEFKKEQGKDLSKDPQALQRIKDSAEKAKIELSSAQQT
                     EINQPFITQGDGGQPLHLTMTLTRSKLEELVDPLIKKSFEPVKKALKDAKLDPKDIED
                     VVLVGGQTRMPKIQDEVEKFFGKAPHKGVNPDEVVAVGAAIQAGVIGGDVTDVVLLDV
                     TPLTLGLETLGGVRTALIDRNTTIPTSKSQVFSTAADNQTQVEINVLQGEREMAADNK
                     SLGRFILDGIPPAMRGMPQIEVTFDIDSNGILHVTAKDKNTSKEQKITIQNSTNLSDE
                     EVERMKKEAEEHAEEDKKKKELVEAKNQANSVAFEMEKQLKEYGDKLEAKDKEQIEKD
                     VQALKDLAAKDDTTKEQLEKAVEETYKSAQKIGEVMQKAQAEAAKTPDAETKTEGDDA
                     EDSAKKDDKKSKKSAKDDGEVEEGEVVKE"
     gene            2968..5019
                     /locus_tag="PKH60_01295"
     CDS             2968..5019
                     /locus_tag="PKH60_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16716.1"
                     /translation="MGSFYRFLPAQVFAQELTSPPANILLVNQVRGAECCQAGSSSTL
                     LQQLTIAQSLNLPTTFSLRYDVLNDLDFLAIIRATQDSDLIRWGLFFEVTPMLAEDAE
                     VPYDGTPATWSRAQHAFPLGYTPDERLRLLNTVMAKYLEVFQEKPELVTAWMLDATTA
                     QLLKNTYGVNLVQITREQWGTDSYTLYGGPVHYPYRASNSWLLAPSQSDANILVIRQT
                     ITDPLWNYGDRFSRFTSQPNDFTQDGKSIEYFRSLLEQTINQPVGQRGWGLIGLENSM
                     EDDYQQLYSQQLKIVAELRDQNKLQVTTPNQLTKTKRTSVEMYTGSDLVRGTNAKVAW
                     ITTPVYQARVRLDENQLYIDDLRLYSESISDPYVNSPARSRGYWITPFLLDGSRFNQA
                     ENQENYQAWEKGVISPLDPTNDVSSSPQRLELGSVNQIESKLWNEYWSKTNNNVQFFD
                     GEKKIIFSSNSILLENWTIDEIKYVAPSEITPPTTFTREENSFNLTWSNLEKDAWKLI
                     GNCEDDSCELSFSISAQAAIDSRNLFPAFMFPEQLVNVVDSAKSTIYLHNQYVVKGKQ
                     LARIVFIPRSTSGISVEIDHPLKFQTSNTLSLDEVRPDMLSQPVQFYDFSALEPGETM
                     ISTSDYDLNFSASVSVAPDCKKEILVCATHPKWLIWYIQSFIQSRVQLQEQSELPWLK
                     V"
     gene            5040..5711
                     /locus_tag="PKH60_01300"
     CDS             5040..5711
                     /locus_tag="PKH60_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009181213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ16717.1"
                     /translation="MKLSVIVPVFNEEKTVGKMLSKLIKVADVYEVIVVDDGSSDNSA
                     KEINKITSKKIQLFSKKNGGKGSAIRFGLTKVSGSHVLIQDADLEYDPEDIPILTEPV
                     KKGKAEVVYGSRFLGPHLNLLYWHRVGNGLLNFLINILFNTTLSDMETCYKLIPTEKL
                     RAINLKANDFDIEPEITCRLLKRGIRIYEVPISYVGRDHSQGKKITWRDGFAAVWVIL
                     RERIF"
     gene            5721..8045
                     /locus_tag="PKH60_01305"
     CDS             5721..8045
                     /locus_tag="PKH60_01305"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HNQ16718.1"
                     /translation="MWKIVLSRWAKYWFPISIGLLLISLRFYPLLLGKSLLFGDNYSL
                     MVPGKLFSAHWLSRGIVPLWNPTQFAGISWVGDINQSLLYPSTILFVVFSAGTALSIT
                     LVGHLLFSFVGAYFLARELSPKITYIGWLLSAVLWSFSPQLVGAMNNLATLQSLSYVP
                     WIVLSCLHFSRRRRNSCWYLPLLITLQLLGGYPQHVLFSVLFGVTLSWLLQDKLSVKK
                     LSAQMQYFGRWSAVGAITLLLSAFVWLPFLQNLNQSTRTLQTAEQAQTGSLRLDDLIK
                     IVTPTFFDNPSVGYKWGPGWNQPTNLVVYFSWVGLIATLWLLLQAKKQKLDLILFGSI
                     LVCLLFSFSESLPFFSLLQGMPIIGSSRGVTPILSIATLAGSLLVSRALLLMKLSPVT
                     QRKYFIVVLLMTLLFGLQYIYSKFNFTQIWTFANSVLGGAFDNSMFHTIEKDRVLSNS
                     ILFSLSIQSLLLFLTSWFLLHKRKMYIVILIICFDLVFFTKQYYVFGPAAFYEPPKVT
                     EALVRVEEELGHNFRLLTRNYNAPYADFGAYADALIVRQPFSDSFVDAQELSNFSVAL
                     KMKELLTPGWNTPFNIPTINGYTTLLPASISIDFSNTSSEHGINRLPEISTTNGQLKK
                     WAVGYYLVDTWYPDYGELFPENLVASGEGWKLYQLPNTLSRIRYGSGESAEISAFHEN
                     SNELVMTIESDREQDLVVADRYDQDWKAIVNGKSVKVSDVDGMRSIPLELGMNQIRLW
                     YSPTLFYVGLFVSGLTGITLLLWIAVRSSLWQTK"
     gene            8094..8954
                     /locus_tag="PKH60_01310"
     CDS             8094..8954
                     /locus_tag="PKH60_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNQ16719.1"
                     /translation="MGTKRDYYEILGLTKTASDAEIKAAYRKLALKWHPDKNPDNKKD
                     AEEKFKEINEAYQVLSDAKKKQSYDQFGHAAFDAASGMGGNPFAGGFRQGPFTWSYTT
                     SGQNPTGDFDFSDPFEIFEQFFGGGFGQQVQRRPRYSLHIDFFEAIRGVTKEVTIEGK
                     KHTIKVPAGANDGTRLRFDDFDITIDVGSHAHYKREGNDLFMDHSIPFSLAALGGTTE
                     VKTVDNSLKLKVRPGTASHTMVRLRGEGVPHLQRGGKGDLYVRLIISVPEKLNREQQK
                     LIKELQELGV"
     gene            complement(8960..11179)
                     /locus_tag="PKH60_01315"
     CDS             complement(8960..11179)
                     /locus_tag="PKH60_01315"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ16720.1"
                     /translation="MLSLAWIATLLALLGNLLLGLVVFLKNPKSATNVFFTIFSLVLS
                     LYLLFNQLSLENLDPEIKLSWMRSVMVLGTMMNISYFFFVITFPLNQLEFSYKKTFIS
                     IIYTLFVAILATTPLLFSGIDVNNNQPIPGPAMAIFLIHTATFLGGGFFILIKKFRRS
                     TGVIKSQIRLVLVGTITMFLLILITNVAFVFVLNNTAFVGLLPLYTTIFIAFTAVAII
                     RHKFLDIGLLAVRAVTYALLILIITSFYALFLFTITSHVPKSIQSQISVAIAILIAFS
                     FNPLKQLIQNLTQKLFYKKSYSPQVLLEELGQILRSNISLSHLTKKVLKELTESMSIA
                     DAAFVITNENSIYATEKVGNEDYFHFDVESVNLLAQKAKTKPLIFEDLEEGVVKDTLR
                     KFHAAVVVPITVKNVRHGILVLGQKSSGDIFNSSDIQVLEIFTHQLSIAINNSLAYEE
                     IREFNVTLKNEVDHATKNLKAANERLKELDQVKDEFVSIASHELRTPLTSIRNYQWML
                     LNDKGGKLTEKQRYYTERSYQSTNRLTKLVTDMLNVSRIDSGRILLDVRKIQLPEMTN
                     DILIELQLKIKEKNIKIVHQYSTEKALPAVLADPDKIKEVIINLIYNALKFTPEKGTI
                     EISYKHQKGFVSMIIKDSGIGIDKSYLSALFTKFGFVKDSLQSNHPSTESTGLGLYIS
                     KSIIELHGGKIWAESEGENQGSTFIFTLPTYNEEQYKLLHKRYKKEKDAGLLHNSIS"
     gene            complement(11255..12286)
                     /locus_tag="PKH60_01320"
     CDS             complement(11255..12286)
                     /locus_tag="PKH60_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNQ16721.1"
                     /translation="MKTHEVITSVATELAKIRHPQNRTPLEADVAPIVAELTARVDPN
                     KPIKAVTDFLDEATFIELTSARNQLLITKEQQQLLRDTVVGFFGLSVGSHAALTWMME
                     SRATNCIISDPDVIDVTNLNRLRLGVESVGKKKIDVVSQALVAINPFSHVITSDQIDY
                     ESVRQQFTGETPVQIVVDAIDNLQGKIWLRQLAKEFSLPLISATDVGDNVFLDIERYD
                     LSPQPDFFLGRVPGIENLSLDALTPIERIQLSMKIVGLEHNSESMLQSLLNIGVAIPT
                     WPQLGATATIAGGITCTAIKKIVLGENVVSGRYIFSLDEILDKSFNDSGRIADRDQQI
                     ANIQTRFGG"
     gene            complement(12302..>12604)
                     /locus_tag="PKH60_01325"
     CDS             complement(12302..>12604)
                     /locus_tag="PKH60_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16722.1"
                     /translation="AVFEHPDLQQHLTGRFSTEKMQDDNQDHYLHIRLELKPSRRPSK
                     YLAEKTRSIFIKTLKELNSEYNQVEDKFGTKMHPTFKFHRFQHPEFFPEGKTKKMG"
     assembly_gap    12605..12704
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12705..13460)
                     /locus_tag="PKH60_01330"
     CDS             complement(<12705..13460)
                     /locus_tag="PKH60_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HNQ16723.1"
                     /translation="MNTVLQQYLESPLNSYGKQIGDTAQLKKDADQKALKLFSLAAKR
                     IPAYKDFLQQFSIKPELIKTIADFSEIPYTTKDNYILTYPIEQRCWDGDLTQTHMFAA
                     SSGSTGEPTFWPRQLDQEVEGALIHEYLFDKVFSVRSKKTLFVNSFGLGNWIAGMYTQ
                     MCVYLNRLHDLPMTIANPGYNQDETFKVIRAFSHFYEQTIIACHPPIIKMMIENGAKS
                     GIDWSKLNVRFLGAGEGFSENWRDYLVALVQQTD"
     gene            complement(13457..15139)
                     /locus_tag="PKH60_01335"
     CDS             complement(13457..15139)
                     /locus_tag="PKH60_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HNQ16724.1"
                     /translation="MMHDVGTMQLTKLVNKLATIFNTPQQIELDGESTLSLSDVYAVA
                     NFNSTVLLSPNPEVLQRIKAVRSAMLEQIESGVPIYGVSSAYGGQAGRVLNEGGTTDR
                     YKKAKQISQAIVHVDVSTGPEIPINIVRAAMLLRVNMLLPGYSSIRLELLETIVQLLN
                     KGITPVVGFYGTLGASGDLAHNGRLASVLIHDPSAYVRTKNGEKTFASKALKANGIPK
                     IDLEPKEGLALVNGDNFSSAAAVIQTLDAIQLMLVNLGSAAMMVQALQGSNRNFHPLL
                     ADVRPHEGQRFVADSLRKLLYQSQLVVDELNGPKRRKKHTIIQDSYSIRCLPQYLGPD
                     WETLDSIWETIATNCNSVSDNPIWTTPETVLPGEKPYQWVSGGNFLAMYMADALDKLR
                     KVLVHIVKQNDRHLARLINPHQNNNLPANLSDPAAISQCTFKGLQTQMGMYDVYATVL
                     ATPVSTAFGVHEEANQDLTSHAMTSALLNQEVLKLTRLSLATNLIASCQAVDLRGGPD
                     QLSPTTKPIYKWIRERVPYVKKEQPLGQYVEAVAAELLDQKLISSLIAGITP"
     gene            15270..15650
                     /locus_tag="PKH60_01340"
     CDS             15270..15650
                     /locus_tag="PKH60_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ16725.1"
                     /translation="MPNQHKILLVDDDELIRDLYSEVLKDANFDVQSAKDGQEGFEML
                     KQGGYDVVLLDLIMPELDGVTVLRKLHDNPPGKPNKHIIVLTNLDFQPTLEEAMELGA
                     ESYLIKSAHTPDEVVEQVTATASA"
     gene            15768..17081
                     /gene="nusA"
                     /locus_tag="PKH60_01345"
     CDS             15768..17081
                     /gene="nusA"
                     /locus_tag="PKH60_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNQ16726.1"
                     /translation="MHNSSSTVIRTEFAAAISQIAKERKIEAESIYEAIRQALVSAYR
                     KQYGLDEEHYYYVILNQENGESRILRAPVITRDEETLEPITWDETKSEDVTPAGFGRI
                     AAQTAKQVILQKIRESEKDHILAEYEEKIGEIVSAQILRMDGRKVILDLGRGYGVMPP
                     EEQMRGEFYRVNSRITVYIKEIRESFKGKVIIVSRADAKLVSQLFAREVPEVGTGAVE
                     VALIAREAGVRTKLAVRSTQEGVDPVGACVGQRGVRVQEVIRELNNEKLDIIPFTEDA
                     KLLLVAALAPAENLKIEIDEKTKTAVVTAPDDQLSLAIGRGGQNVRLAAKLTGYKITI
                     KSASGQVQSHATGDEEYEIDTFEGLSPESRVILIEHKLTTLNDLSRFKEKWMAFELPA
                     DQITLLSDKVVVFEKELEERGESLAEKARLAAKNSNNQTSDSASE"
     gene            17091..18569
                     /gene="infB"
                     /locus_tag="PKH60_01350"
     CDS             17091..18569
                     /gene="infB"
                     /locus_tag="PKH60_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNQ16727.1"
                     /translation="MALQPRPPVVTIMGHVDHGKTTLLDYLRKSRITAGEAGGITQHI
                     GAYQIEHKGKKITFIDTPGHAAFNKMRERGAAVTDFVILVVAANDGVKPQTIESIRHI
                     KAANVPVVVAINKIDLPNVYPDVAKGELAQHGIQVVDMGGDVEAVAISALKGTGVDAL
                     LDTISVMADLQNISGDPEAALRAIVIESAMDARKGVTASVIVQQGTLRGRQLVQTEEV
                     SGRVRMLTNDKGESLVSVGPGCPAELIGLDKVPAVGSLIWEAGQEQPTLVSATTADAT
                     TPIPQTNTNDVDYNFDDLLGEKQKLPLIIRADVEGTLEAIINNLDDESIDLLEAGVGV
                     ITEADLDLAESSKAPIIAFHTKVPKHIVTLAKQRGIKIKMYQVIYELIEDLQKQMLKL
                     MEPTIDEVVTGEAEILQIFEMKGERIAGIRVKTGEIKKNDWLHLKREEEILFNPVIKT
                     MRHGKDELDRVTAKSEAGFTFKNRKLDFKVGDRIIAYKIENE"
     gene            18668..18937
                     /gene="rpsO"
                     /locus_tag="PKH60_01355"
     CDS             18668..18937
                     /gene="rpsO"
                     /locus_tag="PKH60_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNQ16728.1"
                     /translation="MALTKPQKIQVIKDYQLFEGDTGSPEVQVALLTASIQQLAEHLA
                     SHKKDNHSRKGLLKQVAKRRRLLNFLLKHDEQRYRKLIERLGLNK"
     gene            19080..20870
                     /locus_tag="PKH60_01360"
     CDS             19080..20870
                     /locus_tag="PKH60_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16729.1"
                     /translation="MIENPRPETESGLLAELEHFQEFLGNPERVLGFPAEALFRLDIL
                     TKNYPSVFYPTSNVWNKKYRLVELVDPQQPGHPFSNVTGYSFTETQWLKRKYPDSLEI
                     AQAEVGLAIQAYCLLALNLLRKAGRQASITPVAREQGVLTLGQLIEQFADFQHQVSQA
                     ISDLEQQGEINTNWLSISESDLDHSIVKLALLVRIKEQINLRETTGWTQISNFLDQVA
                     EYEPIDADDNFKIVVTGALLNISNSGLHTVSFRIREYLCTSILGLSGVFQFDQILELL
                     RARETRKQAEIRKEVARQRANTKLERLRGKGSLEGRSLLPQPPEGVNTVDWLSRQEWY
                     RKRIIQGAAKNADQSELSRAYPNNHYLYEDEYYQKTLEKARKIEAVKPGLIAFLLRFG
                     VTQLLRYEEDDLLNMYANYQFGKSDVPLSDYYLHLCDTHDHNNAHLVNGKALPSEYRK
                     QGKVKPLFCEFDSPLELYKILMFVASITIQNPKSVSIRAHGGVGTISYPVAITTTMVD
                     LIIGRIQAIVGKDERIAVLVESCHAADRSNYGSSFAEHISELGMGVFAPEGLSYGVKS
                     AEIDSDGYVTDVLFKAFSTKHLKGKKIPAR"
     gene            complement(20894..>21258)
                     /locus_tag="PKH60_01365"
     CDS             complement(20894..>21258)
                     /locus_tag="PKH60_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16730.1"
                     /translation="LQKIDAPQDITQAMSQQMKAEREKRARVLEAQGIKEAAILTAEG
                     EAQSVKLRAEAQANAVTQVAQAAEKNFTPRAEKLKRLEVSEKVLGDGATKYVIPSSAD
                     IVNVLNLDGDTKLVPLKK"
BASE COUNT         6373 a         3955 c         4660 g         6170 t
ORIGIN      
        1 aaaactacag ttagtcatgt cactcacgat ggtcaggcag tcgctggtat tggcaaattt
       61 atggttgggg tgggcgcagt tatcgaatta ggtacatctg gtactttttt ggtaatgaaa
      121 cgacaagatg aatttcatca tggagcctgg gaaatcgtat atggcagatt ggatcagcat
      181 gaagatttgg taacaggttt gaagcgagaa gtacgagaag aaaccgggat ttcacaactt
      241 gaagtgctcg atcattactc acaatggcat ttttatcgag gcaaaaaaac cgcagagacc
      301 gaagtaattg gaatatcatt ttcttgtcgg actaatcaag aagaaattaa gctctccaca
      361 gagcactcgg aatatgcttg ggtaactgct gaggaaatgt tgaaattgtc aacggtgcca
      421 gatattcatg cagatatgaa aaaatatctt gaataccgtg accggcaagc caagctagaa
      481 cagtctcagg cggtagccac tcgagcatta gcagactacc aaaatttagt acgtcgccat
      541 caagatgatc ggcaaaaatt cgctcagttg gcgaatttag attttattac ttctctacta
      601 gaacccttag aacatttgca gcttgcctca gctcaactaa atgataaggg attacagatg
      661 atcgttgcgc aattagttga gagattaaaa gctcagggtt tagaaccaat taatcccatc
      721 ggattgcctt ttgatgtgac cacaatggaa gcagttgatt ctaagggaga aggtaaaata
      781 gttgaaaatg tagttaaaat tggatggaag ctgaacgatc acgtcattca gcatgccaaa
      841 gtaggtttgg tttgaactaa ttagcagtct attgacaaga gtgctaattt ggtgcatact
      901 tcaccgcgta acaacatact tagtttgtag aaaggactat atggcaagtt caggcaaaat
      961 cattggtatc gatttgggta ctactaattc cgcagtggcg gttatggaag gtggttcacc
     1021 taaagtaatc cccagcgcag aaggtcgtaa tacatttcct tcaattgttt cttttaaagg
     1081 tagtgacgta tctgttggag atatagccaa acgacagatg gtgctcaacc caaagaagac
     1141 tgtcaactca gtcaagcgat ttatgggacg taagctaaat gatggcgcag tcaaagctgc
     1201 actcaagcac gtagcttatt cgattgtcga aggtaaagat ggcatggcaa tggtggaaat
     1261 cgatgataaa aagtattctc cacaggaaat ttctgccaaa atcttagcaa aagctaagca
     1321 tgatgctgaa agttatttgg gaggaacagt agatcgtgct gtaattacag tacctgccta
     1381 ctttgatgat tcgcagcgtc aggctacaaa acaagctgga gaaatcgccg gcttgacagt
     1441 agagcgaatt gtaaatgaac ccaccgccgc cgcattagca tatggtttag ataaaaaagg
     1501 ttcaaagact attgcggtgt acgatctagg tggtggcacg tttgatattt ctattttgga
     1561 aatcggtgat ggtgtctttg aagtgaaatc taccaatgga gacacgttcc taggtggtga
     1621 tgattttgat aacattatta ttgaatggat cgttgaagaa ttcaaaaaag agcaaggaaa
     1681 agatttgagc aaagatcccc aggctttaca aagaattaaa gactcagccg aaaaagctaa
     1741 aattgaactt tcaagtgctc aacaaaccga aattaatcag ccattcatta ctcaaggtga
     1801 cggtggtcag ccattgcatt taactatgac gctgacgcgt tcaaagcttg aagaattagt
     1861 ggatccttta attaagaaat cttttgaacc agtcaaaaag gcgctcaaag atgccaagct
     1921 agatccaaag gatatcgaag atgtggtgct agttggtgga cagactcgta tgccaaaaat
     1981 tcaggacgaa gtggagaagt tttttggcaa agctccccac aaaggtgtga atcctgacga
     2041 agttgttgca gttggtgcgg ctatccaagc tggagtcatt ggtggcgatg tcactgatgt
     2101 ggtgctgctt gatgtcacac cattaacttt aggacttgaa actcttggtg gggttagaac
     2161 cgcattaatt gatcgtaata ccacaattcc taccagtaaa tctcaagtgt tttctactgc
     2221 ggctgacaat cagactcagg tggaaattaa tgtgctccag ggtgagcgcg agatggctgc
     2281 agacaataaa tcgctcggtc gctttattct tgatggtatt cctcccgcca tgagaggtat
     2341 gccgcaaatt gaagtcacat ttgatattga ttcaaatgga attctacatg tgactgccaa
     2401 agataagaat accagtaagg agcagaagat aacgattcaa aactcaacta atctttctga
     2461 tgaagaagta gagcgcatga agaaagaagc tgaggaacat gcagaagaag acaagaagaa
     2521 aaaagaactt gttgaggcaa aaaatcaagc caattcagtt gcttttgaaa tggaaaagca
     2581 gctcaaggaa tatggagaca agctagaagc taaagataaa gagcaaatag aaaaagatgt
     2641 gcaagcgctc aaagatttgg cagccaaaga tgatactacc aaggaacagc tagaaaaagc
     2701 ggttgaagaa acctataaat ctgctcagaa aattggtgaa gtgatgcaaa aggcgcaagc
     2761 agaagctgcg aaaactcctg atgcagagac aaaaactgaa ggtgacgatg ctgaagattc
     2821 tgccaaaaaa gacgacaaaa aaagcaagaa aagtgctaaa gacgatggag aagtcgaaga
     2881 aggcgaagtc gtaaaagagt aataaggata catttggtcg ctgctgtctt gaaaagtcta
     2941 cagagggtag ggctgtttgc tctgatagtg ggatcgtttt atcgatttct gccagctcaa
     3001 gtatttgcac aagaattaac ctcacctcca gccaatatct tgttagttaa ccaagttagg
     3061 ggtgccgagt gttgtcaggc aggtagctca tccacattgt tacagcaact aactattgct
     3121 caaagtctta atctacccac aacgttctct ttgcgatatg acgttctcaa tgatctagat
     3181 tttcttgcaa taattagagc tacgcaggat tccgatctga ttcgttgggg tttgtttttt
     3241 gaggtaactc caatgcttgc ggaagacgcc gaagttccgt atgacggtac gccagcaact
     3301 tggtcaagag ctcagcatgc gtttccattg ggttatacac cagacgagcg attgcggttg
     3361 ttgaatactg tgatggccaa atatttagag gtttttcagg aaaaacctga gttggttact
     3421 gcgtggatgt tggatgcgac aaccgcacaa ctcttaaaaa atacatatgg cgttaatctg
     3481 gtgcagatta ctagagaaca atgggggact gatagttaca ctttgtatgg cggtccggtt
     3541 cattatccgt atcgcgccag caacagctgg ttgctggcgc cttcacaatc tgatgccaat
     3601 atcttagtaa ttcgtcaaac cattaccgac cccctctgga attatggtga tcgtttctct
     3661 cgttttacga gtcaaccaaa tgatttcact caggacggaa agtcgattga gtattttcgt
     3721 tctctgttgg agcagacaat taatcaacca gttggtcaac ggggatgggg attaattggt
     3781 ttagaaaatt caatggaaga tgactaccaa cagttgtatt cacagcagct aaaaattgtc
     3841 gctgagttga gagatcaaaa taagcttcaa gtaaccacgc ccaaccagtt gacaaaaaca
     3901 aaacggacca gtgttgaaat gtacaccgga tcagatttag ttcgaggtac gaatgcaaaa
     3961 gttgcttgga ttactactcc agtttatcaa gcacgagtgc gactagatga gaatcaactg
     4021 tatattgatg atctgaggtt gtactcagaa tccatctccg atccatacgt gaattctcca
     4081 gcacgttcaa ggggatattg gatcacccca tttttattag atggatcacg attcaatcaa
     4141 gctgagaatc aagaaaacta tcaagcgtgg gaaaagggcg ttatttcacc gcttgatccc
     4201 acaaacgatg tttcctcatc cccacagcga ctagaattgg gttcagtaaa tcagatcgaa
     4261 tctaaacttt ggaatgagta ttggtcaaaa accaacaaca acgtgcaatt ctttgatgga
     4321 gaaaaaaaga taattttttc ttcgaactct atcttgctag agaattggac gattgatgaa
     4381 attaagtatg tagcgccttc tgaaattacc ccacccacaa catttactcg tgaagaaaac
     4441 tcgtttaatc ttacttggtc taacctagaa aaagatgctt ggaaattaat tggaaactgc
     4501 gaagatgatt cttgcgaatt atcttttagc atttctgcgc aagcagcgat tgactctcga
     4561 aatctatttc cagcattcat gtttcctgaa caactggtta atgttgtaga ttcagccaaa
     4621 tctactatat atttacacaa tcaatacgtg gtgaagggaa aacaactagc tcgaatagtg
     4681 tttatcccaa gatcaacaag tggcatatct gtcgaaatcg atcatccgct caaatttcaa
     4741 actagtaata ctctcagtct tgatgaagtg aggcctgata tgttatctca acctgttcag
     4801 ttttatgatt tttctgctct cgagccgggc gaaactatga tatcaacttc agattatgat
     4861 ctcaattttt ctgcttcagt ctctgttgcg cctgactgca agaaagaaat tctagtatgt
     4921 gcaacacatc caaagtggtt aatttggtac atacaatcat tcatacagtc gcgtgttcag
     4981 ttgcaagagc aatctgaatt gccttggttg aaagtctaag gtattgtatg atagcaagca
     5041 tgaaactatc tgtaatcgtt cccgtcttta acgaagaaaa aacagtgggg aaaatgttgt
     5101 ctaagttgat aaaagtcgct gatgtatatg aagttatcgt ggtggacgat ggctcatcag
     5161 acaattcggc caaagaaatt aataagatta caagtaaaaa aattcaacta ttttctaaaa
     5221 aaaatggagg taaaggaagt gcaattcgct tcggattaac taaagtcagc ggatcacacg
     5281 tgctcattca agatgcggat ttggaatatg atcctgagga tattccaatt ttgaccgaac
     5341 cagtcaaaaa aggcaaagct gaagtggttt acggatcaag atttttaggt ccacacttaa
     5401 atttattgta ttggcatcgt gttggcaatg gattattaaa ttttctgatt aatattctgt
     5461 ttaatacaac gttgtctgat atggaaacgt gctacaaact aattcctacc gagaaattga
     5521 gggcaattaa tctcaaagct aatgattttg atatcgaacc agaaataacc tgcagattgt
     5581 taaagcgcgg aatacgaatc tatgaagttc caattagcta tgttggtcgc gatcattctc
     5641 agggtaaaaa aatcacttgg agggatggtt ttgccgcagt ctgggtgata ttgagagaac
     5701 gcatatttta gattagcagt atgtggaaaa tagtactaag tcgatgggcg aaatattggt
     5761 ttccgattag tattggatta cttttaattt cactaagatt ttatccgctt ttgttgggta
     5821 agtcattact ctttggtgat aactattcct taatggtccc cggtaaactt ttttcggctc
     5881 attggttatc gaggggaatt gtgccactat ggaatccaac gcaatttgct gggatttctt
     5941 gggtggggga tatcaaccaa tctttgttgt atccgtcaac catactattt gttgtgtttt
     6001 ctgctggcac tgccctatca attacgttgg ttggccactt actattttca tttgttggag
     6061 cctatttttt agccagagaa ctcagtccaa aaattactta cattggatgg ttgctttcag
     6121 cagtactctg gtcattttcg ccacaattag ttggtgcgat gaataatctt gcaactttgc
     6181 agtcgttaag ttatgtcccc tggatagttt tgagctgttt acattttagt cggaggcgaa
     6241 ggaacagctg ctggtattta ccacttttga taaccttaca gctactcggt ggctatccgc
     6301 aacatgtgct ttttagcgta ctgtttggtg taacactctc atggttgctc caagataaac
     6361 tttcagtaaa aaaactgagt gctcagatgc agtattttgg tagatggagt gcggttggag
     6421 caatcacact tttgctgtct gctttcgtct ggttaccgtt tttacaaaat cttaaccaat
     6481 caactagaac actgcaaacc gctgagcaag ctcaaactgg ttctctacga ctggatgatc
     6541 taattaaaat agtcactcca actttttttg acaatccaag tgttggttac aaatggggtc
     6601 ctggctggaa ccaaccaacc aatcttgtgg tgtattttag ttgggttggt ttaattgcga
     6661 ccctgtggtt gctgttgcaa gcgaaaaaac aaaaacttga tttgattcta tttgggagca
     6721 tccttgtttg tctgttattt tcttttagtg agtccttacc atttttttca ctattgcagg
     6781 gtatgcccat cattggttca tcaaggggag tgactccaat actctctata gcaaccttgg
     6841 cgggctcact gctcgtttcc cgagctcttt tattaatgaa gctttctcct gttactcaga
     6901 gaaaatattt tatcgtagta ctactcatga ctttgctatt tggactgcaa tatatatatt
     6961 caaaatttaa ttttactcaa atatggactt tcgcaaactc tgttttgggc ggagcttttg
     7021 ataacagtat gtttcacaca attgagaagg atagggtact cagcaactca attttgttca
     7081 gtctcagtat tcaaagtctt ttgttatttc tgacctcctg gtttttgtta cacaagcgga
     7141 agatgtatat tgtgattctg attatttgtt ttgatcttgt tttttttact aagcagtatt
     7201 atgtatttgg tccagctgcg ttctatgagc caccaaaagt taccgaagca ctagtacgag
     7261 tcgaggagga gctaggtcac aattttcgat tgcttactag aaactacaac gcgccatacg
     7321 cagactttgg agcctatgct gatgcattga ttgtgaggca gccattttct gattcatttg
     7381 tggatgcgca agaattatcg aatttttctg tggctctcaa aatgaaggaa ttattaactc
     7441 caggatggaa tacgccattt aatataccga ctattaatgg gtatacaaca ttattgccag
     7501 cttcaatatc aatagatttt agtaatactt ctagtgagca tggtattaat cgattgccag
     7561 aaatttcgac tactaatgga cagttaaaaa aatgggcagt tggctattat ttagttgata
     7621 cttggtatcc cgactatggc gagttgtttc ccgagaattt agttgctagt ggggaaggtt
     7681 ggaaattgta tcaactaccc aatacattat ccagaattcg ttatgggagc ggtgagagcg
     7741 cagagatctc cgcttttcat gagaattcca acgaattagt tatgacgatt gagtcagacc
     7801 gtgagcaaga tttggttgta gctgatcgct atgatcagga ttggaaagcg attgtgaatg
     7861 gcaaatcggt aaaagtctct gacgtcgatg gcatgcgctc aattcccctg gagttgggaa
     7921 tgaaccagat tcgtttgtgg tattctccaa ccctatttta tgttggtttg tttgtatctg
     7981 gtttgactgg cattactttg ttgctatgga tagctgtccg aagtagtctt tggcagacaa
     8041 agtaattgtc tgctagactt catcaaactc acttgttaca atgagccctt attatgggaa
     8101 ccaaacgtga ttattacgaa attcttgggt taaccaagac tgcatcggat gctgaaatta
     8161 aagcagccta tcgtaagctg gctttaaagt ggcatccaga caaaaaccca gataataaaa
     8221 aagatgcaga agaaaaattc aaagaaataa atgaggcata ccaagtgctg tcggatgcca
     8281 agaagaagca atcgtatgac cagtttggcc atgcggcgtt tgatgctgcc agtggcatgg
     8341 gcggcaaccc gtttgccggt ggatttcgtc agggtccatt tacttggtca tataccacta
     8401 gtggccaaaa cccaactggc gactttgatt tttctgatcc atttgaaatc tttgaacagt
     8461 tttttggcgg tggttttggc cagcaagtcc aacgaagacc gcgatattcc ctgcatatcg
     8521 atttctttga agccattcgt ggagtgacca aagaagtcac tatagaaggc aaaaagcaca
     8581 ctatcaaagt tccagcggga gctaacgatg gtacgcgcct tcggtttgat gattttgata
     8641 tcactatcga cgtgggatct cacgcacatt acaagcgcga gggcaatgat ttatttatgg
     8701 atcactccat tcctttttct ttagcagctc tgggtggaac aactgaggtt aaaacggtag
     8761 ataattcgtt aaaattaaaa gtgcgtccag gaacagcgtc tcacaccatg gttcgattga
     8821 gaggagaggg ggtgccacac ttgcaacgcg gtggcaaagg tgatttatac gtccggttaa
     8881 ttatttccgt accagaaaaa ctaaatagag agcaacaaaa actcattaaa gaattgcagg
     8941 agcttggagt ctagtccaat taactaattg aattgtgtag cagtccagcg tctttttctt
     9001 ttttataccg tttatgaaga agcttatatt gttcttcgtt gtacgtgggt agagtaaaaa
     9061 taaaggttga accttggttt tcaccttcgg actctgccca gattttacct ccgtgcagct
     9121 cgatgataga tttagagatg tataagccca gcccagtact ttctgtggag ggatgattag
     9181 actgaagcga atctttgaca aaaccaaatt tggtaaacaa cgctgagaga tacgatttat
     9241 ctattccaat tccactatcc ttaattatca tagaaacaaa gcccttttga tgtttatagg
     9301 aaatttcgat agtgcctttt tctggagtaa atttaagggc attataaata aggttaataa
     9361 ttacttcctt aattttgtct ggatcggcta gaactgctgg aagtgctttt tcagtggaat
     9421 attggtggac aattttgata tttttctctt ttatcttaag ctgtaattca atcaaaatat
     9481 catttgtcat ttctggaagt tgaatttttc tcacatccag caatatacgc ccagagtcta
     9541 ttcttgaaac atttaacatg tcagtaacta attttgttag cctgtttgtt gattgataag
     9601 agcgctcagt gtaatagcgt tgcttttcgg ttagtttgcc ccccttatcg ttcagtagca
     9661 tccactggta attgcggata gacgtgagtg gggttcgtaa ctcatgggaa gcaattgaaa
     9721 caaactcatc tttgacttga tcgagctctt ttagtctctc attggcagcc tttaaatttt
     9781 ttgttgcatg gtcaacttca tttttgagcg tgacgttaaa ttctctaatt tcttcgtaag
     9841 cgagtgaatt attaatggca atggaaagct ggtgtgtaaa gatttctaat acctgaatgt
     9901 cgctactatt gaagatatct ccagacgatt tttgaccaag gaccaatatt ccatgacgta
     9961 catttttgac cgtaatagga actacaactg ctgcatggaa ttttcgcaag gtgtctttta
    10021 caacgccctc ttctaaatct tcaaagatga gaggttttgt ttttgctttt tgagctagca
    10081 agttgacaga ttcgacatca aaatgaaagt aatcttcgtt accaactttt tcagtagcat
    10141 aaatactgtt ttcgttagta ataacaaatg cagcatccgc aatagacatt gattccgtca
    10201 attcctttaa cacttttttt gttaaatgag ataaagaaat atttgatcgt aaaatttgac
    10261 caagttcttc cagtaatact tggggagaat acgatttttt ataaaagagt ttttgagtaa
    10321 gattttgaat taattgcttg agtggattaa atgaaaaagc aattaaaatt gcgattgcga
    10381 cggaaatctg cgactgaata gatttgggta catgcgatgt gatggtaaaa aggaacaatg
    10441 cataaaagga ggtgatgata aggataagta atgcgtaggt aactgctcga actgcaagca
    10501 acccaatatc cagaaatttg tgtctaataa tcgctacggc agtaaaggca ataaaaattg
    10561 tagtataaag tggaagtaag ccaacaaaag ctgtgttatt taaaacaaaa acaaacgcaa
    10621 cgttcgtaat taatatcagt aaaaacatcg taattgttcc gactagtact aatctgattt
    10681 gagactttat tactccagta ctgcgtcgga atttcttgat taatatgaaa aatccgccgc
    10741 caagaaacgt tgcagtatgg atgaggaaaa ttgccattgc tggtccggga attggttgat
    10801 tattatttac atctatacca ctgaaaagta atggtgtagt agctaaaatt gcaacaaaaa
    10861 gtgtataaat aattgagatg aacgtttttt tataggaaaa ttctaactga ttgagtggaa
    10921 acgtaatcac gaaaaaaaag taactaatat tcatcatggt gcccaaaacc atcacgctac
    10981 gcatccagga aagttttatt tctggatcca agttctccag cgaaagttga ttaaacaata
    11041 aataaagaga taacactaaa ctaaagatgg taaaaaatac atttgttgca cttttggggt
    11101 tttttaaaaa taccaccaaa cctagtaaaa ggttgccgag cagggcgaga agggttgcaa
    11161 tccaagcaag acttaacata caaaaaaagt atagtgtgtt atggagtgct tgtcgagctg
    11221 cgaagtcaat gatccgcgat gattattggg tgcattaccc accaaaccta gtttgaatat
    11281 ttgcaatttg ttgatcgcga tctgctattc ttccagaatc attgaaggat ttgtctagaa
    11341 tctcatccaa cgaaaaaatg tatctaccac tcactacgtt ttctcccaat acaatctttt
    11401 ttattgcggt gcaagtaatg cctcctgcaa tagttgcagt tgcccccaac tgaggccatg
    11461 tgggaattgc cacgccaata ttaagcaaag attgcagcat cgactctgaa ttatgttcga
    11521 gcccaactat tttcatgctt aactgaattc tttcgattgg agtcagggcg tctaacgaca
    11581 ggttctcaat tccgggtact cttccaagaa aaaaatctgg ttgtggtgaa agatcgtagc
    11641 gttcaatgtc taaaaacaca ttgtctccga catcagtggc agatattagg gggagagaaa
    11701 actctttagc taactggcgt aaccaaatct tgccttgtaa gttgtctatg gcatctacca
    11761 ctatctgtac tggtgtctca cctgtgaatt gttgtctgac cgattcataa tcaatttgat
    11821 cgctagtaat aacatgcgaa aagggattga ttgctaccaa agcttggcta acaacatcga
    11881 ttttcttttt accaacgctc tccactccaa gacgtaatct gttgagattg gtaacatcaa
    11941 tcacgtcggg atcagaaata atacagttgg tagcccgaga ctccatcatc catgtgagtg
    12001 ctgcgtgact gccaacactc aaaccgaaaa atcctactac agtatccctg agtagttgct
    12061 gctgttcttt ggtaattagc agttgattac gagcagaggt aagctcaata aaagttgcct
    12121 catccaaaaa atctgttaca gctttgatag gtttatttgg atcaactcga gctgttagct
    12181 cagcaacaat gggggcaaca tctgcttcca aaggggttcg attttgtgga tgtctaattt
    12241 tagctaactc agttgcaaca gatgtaataa cctcgtgggt tttcataaaa aagccgcctt
    12301 tctaacccat cttttttgtt ttaccttcag gaaaaaattc tggatgttgg aatcgatgaa
    12361 acttgaatgt ggggtgcatt ttcgttccaa atttatcttc tacttgatta tattcgctat
    12421 ttaactcttt caaagttttg atgaaaattg agcgagtttt ttctgccaga tatttacttg
    12481 gtctgcgact gggtttcaat tcaagcctga tatgcaagta atgatcttga ttgtcatctt
    12541 gcattttttc tgtagaaaaa cgaccggtta gatgttgctg taaatcgggg tgttcaaaca
    12601 cagcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatcggt ttgttggact
    12721 aatgcaacta agtagtccct ccagttttca gaaaaacctt cacccgcacc aagaaatcta
    12781 acgttaagtt tgctccaatc aattccagat ttggctccat tttcgatcat catttttata
    12841 attggtggat gacaggcaat aatggtttgc tcataaaaat gagaaaaagc cctaattact
    12901 ttaaaggttt catcttggtt gtagcctgga tttgcaatag tcatcggcaa atcatgcaac
    12961 cggttgagat acacgcacat ttgggtgtac atacccgcaa tccaattgcc taaaccaaat
    13021 gaattcacaa acaatgtttt ttttgaacgt accgaaaaaa ctttatcgaa aagatattca
    13081 tgaatgagag cgccttcaac ttcttgatct aattggcgcg gccaaaaagt gggttcgccg
    13141 gttgaaccag aactggcagc aaacatatgt gtttgggtga gatcgccatc ccaacaccgt
    13201 tgctcaatcg ggtaggtaag aatgtagtta tcttttgtgg tgtaaggaat ctcagaaaag
    13261 tctgcaatgg ttttaattaa ttcgggttta attgaaaatt gttgaagaaa atctttgtag
    13321 gcaggaattc ttttagcggc taatgaaaaa agcttgagag ctttttggtc tgcgtccttt
    13381 tttagttgag cggtatcacc aatttgtttt ccgtatgagt tgagtggtga ctctagatac
    13441 tgttgtaaca cagtgttcat ggtgtaattc cagcaataag gctactaatg agtttttgat
    13501 caagcagctc agcagccaca gcctcaacat attgacccaa gggctgttct tttttgacat
    13561 atggcacgcg ctctctaatc cacttgtaga tgggttttgt ggttggtgag agctggtctg
    13621 gacctcctct aagatcgaca gcctggcaag aagcgattag attggtagcc agtgataagc
    13681 gcgtcagttt gaggacttct tgattgagta gggcggatgt catagcgtgc gaagttaaat
    13741 cttggtttgc ttcttcgtga acgccgaatg cagtgctaac tggtgtagcg agtaccgtgg
    13801 cgtagacatc atacatgccc atttgggtct gcagaccttt aaaagtgcac tggctaatag
    13861 cagcgggatc agaaagattt gctggcaaat tgttgttttg atgcggatta atcaatctcg
    13921 ctaaatgtcg atcattctgt ttcacaatat gaacgagtac tttccgtaat ttatctaaag
    13981 catcagccat gtacatggct aaaaagttgc cgccagaaac ccattgatag ggtttttctc
    14041 ctggaaggac tgtttctggc gtagtccaaa tggggttgtc tgacacacta ttgcagttag
    14101 tagcaatggt ttcccaaatt gaatcgagtg tttcccagtc aggacccaga tattgtggta
    14161 ggcaacgaat actataacta tcttggataa tggtatgttt ttttctccgt ttgggaccat
    14221 tcagttcgtc aactacgagc tgactttgat agagcaattt tctgagtgaa tctgcaacaa
    14281 accgttgacc ctcatgtggt cgaacatcag ccaacaaagg atgaaaattg cggttacttc
    14341 cttgaagagc ctgaaccatc atggctgcac tcccaagatt taccaacata agttgaatgg
    14401 catccaaggt ctgaatcact gcagctgctg aagaaaaatt atcaccatta actagcgcca
    14461 aaccttcttt gggttcaaga tcgatttttg gaattccatt tgctttcagt gctttactag
    14521 cgaatgtttt ttcaccgttt tttgtacgca catatgcact ggggtcatgg ataaggactg
    14581 atgccagtcg gccattgtgc gctagatcgc cagaagcgcc aagtgtgcca tagaaaccaa
    14641 caaccggagt aatacctttg tttaagagtt ggacaatcgt ttcgagtagt tctaaacgaa
    14701 ttgagctgta accaggcaat agcatattca ctctcagcaa catcgcagct cggacgatat
    14761 taattggaat ttctggtccc gtggatacat caacgtgaac gattgcttga gaaatttgtt
    14821 tcgccttctt atatctatcg gttgttccgc cttcattcag cactctgcct gcttgaccac
    14881 cgtaggcact agaaacaccg tagattggaa ctccagattc aatttgttct agcatggccg
    14941 atcgaacagc tttaattctt tgtaaaactt ctggattggg actgagcaga acagtcgagt
    15001 tgaaattcgc cactgcgtac acgtcgctga gtgaaagtgt tgattctcca tcaagctcta
    15061 tttgttgagg tgtattaaaa atagtagcta gtttgtttac taacttagtt aactgcatcg
    15121 tacccacatc atgcatcata ttcacatcgc tttctgagta atcatggacg aaaagcacga
    15181 atagtataca gtatcgcgta gtaatttgtt tgtactcatt gctgaaggga tatgatacta
    15241 tttactagca atatccgtgg gacgaaatta tgccaaacca acacaaaatt ctcttagttg
    15301 acgacgatga gttaattcga gatctatatt cagaagtctt aaaagacgct aattttgacg
    15361 tccaatctgc taaggacggg caagagggtt ttgaaatgct taaacaaggt ggctatgatg
    15421 tagtattact tgatctaatt atgcctgagc ttgatggtgt tactgtcttg cgaaaattac
    15481 acgataatcc accaggaaag ccaaataagc acattatcgt gctcacgaat ttagattttc
    15541 aaccaactct agaggaggca atggagctgg gagccgaaag ttatctaatt aaaagtgccc
    15601 acactcctga tgaagttgtg gagcaagtga ccgcaacagc ttcagcctga agatgaagcg
    15661 gggttgtcac gaggtctagc ttgaggtata ctacattcaa gcttttttgg aaaaaggctc
    15721 accggtgagt ctttttttat tattacagag taaagacaaa ggaaattatg cataactcaa
    15781 gctcaaccgt cattcgtaca gaatttgcgg cagcaattag tcagattgcc aaagaacgga
    15841 aaattgaagc agaatcaatt tacgaagcta tcaggcaagc gcttgtttcg gcctatcgga
    15901 agcaatatgg tcttgatgaa gagcattact actatgtgat tcttaatcaa gagaatggcg
    15961 aatctaggat tcttagggct ccagtaatta ctagagatga ggaaacgcta gaaccaataa
    16021 cttgggatga aacaaaatcc gaagatgtga ctccagcagg ctttggtcgg attgctgccc
    16081 aaactgccaa acaagtaatt ttgcaaaaaa ttcgtgaatc cgaaaaagat catattctcg
    16141 cagaatatga agaaaaaatc ggcgaaattg tctctgcaca gatcctacgc atggatggca
    16201 ggaaagtaat tttggatttg ggtcgagggt atggtgtcat gcctccagaa gagcagatgc
    16261 gtggtgagtt ttatcgagtt aattctagaa ttaccgttta tattaaagaa attcgcgagt
    16321 cttttaaagg caaagttatt attgtgtctc gagcagatgc aaagttagtt agccagcttt
    16381 ttgctcgcga agtaccagaa gttggtacgg gagcagtaga agtggcatta attgctcgtg
    16441 aagctggcgt tcgcaccaag ctggcagtca gatcaactca agaaggcgta gatccagttg
    16501 gtgcctgtgt tggccaaaga ggcgtcagag tgcaagaggt aattcgtgaa ttaaataatg
    16561 aaaaattaga cattattcct tttaccgaag atgcaaaatt attactagtg gctgcattag
    16621 ctccagccga aaatctgaag atagagattg atgaaaaaac aaaaactgca gtagtcactg
    16681 caccagatga tcaactttcg ttggccattg gcagaggtgg ccagaatgta cgtttagccg
    16741 ccaaacttac aggatataaa attacaatca aatctgcatc aggccaagtg caatctcatg
    16801 ccactggtga tgaagaatat gagattgata cgtttgaagg attatccccc gaatccagag
    16861 tcattttaat tgagcataaa ttaaccaccc tcaacgattt atctcgtttc aaagaaaagt
    16921 ggatggcatt tgaattgcca gctgatcaaa ttacacttct ctcggataaa gtcgtggtgt
    16981 ttgaaaaaga acttgaggaa agaggtgaga gtttggctga aaaagctcgc ttggctgcca
    17041 aaaatagtaa taatcaaaca agcgactcag catcggagta aaatagctat atggctttac
    17101 aacctagacc accagtcgtg accattatgg gtcatgtaga tcatggtaaa acgacattac
    17161 ttgattactt gcgcaagtcg cgaataacag ctggtgaagc aggtggcatt actcagcata
    17221 ttggtgcata tcagatagaa cataagggca agaaaattac ttttatcgat actccgggac
    17281 acgccgcatt taataagatg cgtgagcgtg gggcggccgt aaccgatttt gtgatattag
    17341 tggtggccgc caacgatgga gttaagccac agactatcga atcgattcgc cacatcaaag
    17401 cagcaaatgt gccggtcgtt gtggcaatta ataaaattga tcttcccaat gtgtatccag
    17461 acgtggccaa aggggagttg gcccagcatg gcattcaagt ggtggatatg ggcggtgatg
    17521 ttgaagctgt ggctatttct gcattaaaag gcactggcgt tgatgcgtta ttagatacca
    17581 tttccgtgat ggcagatctg caaaatataa gcggcgaccc cgaggcagct ttgcgagcca
    17641 ttgtgattga atcagctatg gatgctcgca agggagtgac tgcctcagtg atcgttcaac
    17701 agggaacgtt gcgaggacgt caattagtcc aaacagaaga agtctctggc agagttcgga
    17761 tgctcaccaa cgacaaaggt gaatcattag tgtcagttgg accagggtgt cctgctgaat
    17821 taattggttt agacaaagta cccgctgttg gttcactcat ttgggaagct ggtcaggaac
    17881 aaccaaccct cgtttcagcc accactgccg atgcaaccac gccaattccc caaacaaata
    17941 ctaatgatgt tgactataat tttgatgatt tgctgggcga aaagcaaaaa ttacccctca
    18001 ttattcgagc cgatgtagaa ggtacattag aagcgattat taataattta gatgatgaga
    18061 gtatcgattt actcgaagca ggggtagggg taattactga agcagacttg gatttagccg
    18121 aaagtagcaa agcaccaata attgcgtttc acactaaggt acccaagcac attgtcactt
    18181 tggctaaaca gcgcgggatc aagataaaaa tgtatcaagt tatttatgag ttgattgaag
    18241 atttgcaaaa acagatgctc aagttgatgg agccaactat tgatgaagtt gtgactggtg
    18301 aagcagaaat tctacaaatt ttcgaaatga aaggcgagcg gattgcaggc attcgggtta
    18361 aaactggtga aattaagaag aatgactggc tgcatctaaa aagagaagaa gaaattttat
    18421 tcaatccagt catcaaaact atgcggcacg gcaaagatga actagacaga gtcactgcca
    18481 aaagtgaagc tggtttcacg tttaaaaata gaaaacttga ttttaaagtt ggcgatagaa
    18541 tcatcgccta taaaatcgag aacgaatagt gtataatcgc cgactcgaag agtatacatc
    18601 atggtcactc tgggaagcag gtctgtaact tgcacctaag aggcattaat cttgaaagga
    18661 acataccatg gcactaacaa aaccacaaaa aatccaagtt ataaaggatt accaactttt
    18721 tgagggcgat acaggatctc ccgaggtgca agttgcttta ttaacagcct caattcaaca
    18781 attggctgag catttagctt ctcacaaaaa agataatcac tccagaaaag gtctgctcaa
    18841 acaggtggcc aaacgtcgtc gattactcaa ctttttgctt aaacacgacg aacaacgcta
    18901 ccgcaagtta attgagcggt tgggcttaaa caaataaacc gttgttcaac accacatact
    18961 tttgttaatt tgatagttta gtgtaaacca ttgccttacc tagagacgta cttaagtgta
    19021 aacctaagtt gtatttgagt gatgagatct aacggatact tttatgttaa tatggtttgg
    19081 tgattgagaa cccaagacca gaaacagaga gtgggctgct cgctgaacta gaacactttc
    19141 aggagtttct aggtaatcca gaacgagtct tgggttttcc tgcagaagcg ctatttaggc
    19201 ttgatatcct cacaaaaaat tacccgagtg ttttttaccc aactagcaat gtttggaata
    19261 aaaaatatcg gttggtagag ttggttgatc cacaacaacc tggacaccct ttttctaatg
    19321 taacaggcta ctcttttact gaaacccaat ggctcaagcg taaatatccc gattcactag
    19381 aaattgctca ggcagaagtg ggtttggcaa ttcaggcata ttgtttgttg gctctaaatt
    19441 tgcttagaaa ggcggggcgc caagcatcaa tcacgccggt tgctcgtgag cagggagtcc
    19501 taactctcgg ccaactaatt gaacaatttg cagattttca acatcaggta tctcaggcca
    19561 tttcagatct tgagcaacag ggagaaatta atacaaactg gctttcgata tcagaaagcg
    19621 atttggatca ctctatcgtc aaattggcac tattagttcg aatcaaagag caaattaatc
    19681 taagagaaac cacaggctgg actcaaatct caaactttct cgatcaagtt gctgaatatg
    19741 aaccgataga tgctgatgat aatttcaaaa tagtagtaac gggtgcgtta ttgaatattt
    19801 caaattctgg attacacact gtttcgtttc ggattagaga gtacctatgc acgtcgatct
    19861 taggtttatc tggagtgttc caatttgatc aaattcttga attgctgagg gcaagagaga
    19921 ctcgcaagca ggctgagata cgtaaggagg tggctaggca aagagcaaat actaaactcg
    19981 agcgactgag gggtaaggga agcttggagg gaagatcgct tttgccacaa ccacctgagg
    20041 gagttaatac ggttgactgg ctcagtcgac aagaatggta tcgcaagcga attattcagg
    20101 gagccgcaaa aaatgctgat caatctgagc ttagtcgagc gtatccgaac aatcattatt
    20161 tgtacgaaga tgaatactat caaaaaacac ttgagaaagc tcgaaaaata gaggctgtaa
    20221 agccaggact cattgctttt ctgttacgtt ttggtgtgac tcaattactt cggtacgaag
    20281 aagacgattt gttgaatatg tatgcaaact atcagtttgg gaagagtgat gtgccgctat
    20341 ctgattacta tttgcatctt tgtgatacgc acgaccacaa caatgcacac ctagtcaatg
    20401 gaaaagcact tccgagtgaa taccggaagc aaggaaaagt taaaccactt ttctgcgagt
    20461 tcgactcacc tcttgaattg tataaaattc tgatgtttgt tgcgtcgatc acgatacaaa
    20521 atccgaagtc ggtttcaata cgggcacacg gtggggttgg cactatttcg tatccagttg
    20581 ctataactac cactatggtt gatttaatta tcggtcgaat tcaagccatc gttggaaaag
    20641 atgaacggat cgctgtacta gttgagtcat gccatgctgc tgatagatct aactatggaa
    20701 gttcatttgc cgaacacatc tcagaattgg ggatgggagt ctttgcgcca gaggggttat
    20761 cttatggcgt gaaatcagct gagattgatt ctgacgggta tgtcactgat gtattattca
    20821 aagcttttag caccaagcat ctaaaaggaa aaaaaatacc agctagatga aaccaggaca
    20881 aactgggatc atactatttt tttagtggaa ctagcttggt atctccatca agattgagta
    20941 cgttgacaat gtctgccgaa gaggggataa cgtatttagt ggcaccatcg cctaagacct
    21001 tttcgctgac ttctaggcgt ttgagctttt cggcgcgcgg agtgaagttc ttttccgcag
    21061 cttgagctac ttgtgttacc gcgttagcct gggcctccgc gcgcaacttt accgattggg
    21121 cttcaccttc agcagtcaga atagccgctt ctttaatacc ctgggcttca agcactctag
    21181 ctcttttttc acgttctgcc ttcatttgtt ggctcatggc ttgagtaata tcttgtggag
    21241 catcaatttt ttgcagtt
//
LOCUS       DAOQZP010000012        21009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_2894_length_21009_cov_5.307960, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000012 DAOQZP010000000
VERSION     DAOQZP010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 21009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21009
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2894_length_21009_cov_5.307960"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..510
                     /locus_tag="PKH60_01370"
     CDS             100..510
                     /locus_tag="PKH60_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HNQ16731.1"
                     /translation="MKTYVVSAKVKHGDQHGRSIGHPTINLDSTLWPADLQPGVYASK
                     VTLGEKQYLGALYFGPRSIKAETKNILEITLFEFSDTIYGEVVSVLVGDFVREPIEYQ
                     PGKQNENALKEQIADDVSKVMQLGRLRWPQLAIL"
     gene            517..1374
                     /locus_tag="PKH60_01375"
     CDS             517..1374
                     /locus_tag="PKH60_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNQ16732.1"
                     /translation="MPDKYSALWLSHSSIRDFQQCPRSYYLNNMYRDPQTNKKIQLVS
                     PPLSLGSAVHQIIEHLSDLPVDKRFETSLVELFDKIWPNFTGLKGGFSDQTTEERYKE
                     RGKAMLRTVMQHPGPLANKAIKVQEDLPQYWLSESDEMILCGKIDWLEYIPETDSVHI
                     IDFKTGKNNEEGSLQLPIYLLLVSNVQKRKVAGASYWYLESGANLTEQTLPTAEAAYD
                     QVMLVAKQIKLARKLDRFKCPQGEIGCKFCRSLEKIVSGEAIFVGQGEYGKNCYVLPK
                     DQMQEAKLL"
     gene            1384..2856
                     /gene="crtI"
                     /locus_tag="PKH60_01380"
     CDS             1384..2856
                     /gene="crtI"
                     /locus_tag="PKH60_01380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HNQ16733.1"
                     /translation="MAKIIVIGAGIGGISAAAHLAKAGHRVTVLEKNDQPGGRAEIFQ
                     EKGYTFDRGPSWYLMPEVFDKWFADLGFDREDYYQIITLNPQYKVYYQDKTQLEIGID
                     REKNLHKIARLQPGSLPKLRAYLRQAQTKYDLAMETFLYKNADSPLDFLQYWRLLPQA
                     LQLNLFGTMQHYIERYITEPKLQQLLMYNLVFLGCSPYNAPALFSLMAHVDLNVGVYY
                     PAGGFLNMVASMVSIAKQLGVRFEYNQPIKKINTTSGLVTSLETDIKTWQADYVVSNA
                     DYVHTEDLLSDQTMRQHSDGYWKRKINGPSAFLLYLGINRKIPELMHHTLYFTEDWQK
                     HFSDVFNHKQWPQNPSIYINRPTATDASLAPKGHDTLMILVPVASGLADSKSLRESYG
                     DYIIKYIEKNLNIEFHSAITVKKIVTISDFEKRYNSFEGNAFGGLAHTLFQSSIWRPS
                     NRHKKLSNLFFTGAGTVPGIGLPTSLISGYLVNDRIAKLK"
     gene            2853..4325
                     /locus_tag="PKH60_01385"
     CDS             2853..4325
                     /locus_tag="PKH60_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HNQ16734.1"
                     /translation="MTYLYILISIIIFPFIIGLTSGFRFYFNRKLVKTLFLTATPFLI
                     WDWWATTQGHWSFNPTHIIGVTFLHLPIEEIAFFFVAPFACLFIYAAGEKYLQRRYIS
                     LPFFFWVLITGASMLFLVVGFPKPYSTLVFGLSTLLLLYSYQRFRSQFTTPILIYFIS
                     SFIFFLLFNSLLTMVPVVSYAPWAILNLRVGTIPIEDFWYNFLLLSFTLIIYRVMSNK
                     HTNAQAVFQKGSTTYYTASQFFSPKIREKVTTLYAFVRLADNYVDQLPEKKKELEAFF
                     AEYHNEIRSPGKARQVISNFVSLMKNTGIKPAWVEAFLDSMIQDITKKTYANFGDLSK
                     YIYGSAEVIGLMMSRIFGLSSDHDPAAAKLGKAMQYCNMLHDIDEDLSLGRIYLPLSV
                     LQKYKLEPFSKVMAQKKPKQFRLLMQQELSRCLKWFDEAEKDISFIPRRERVAVKTAS
                     AMYRWTAEQLYKNPQLVFQKKLKPSKLRVILTAISFSIKG"
     gene            4330..5409
                     /locus_tag="PKH60_01390"
     CDS             4330..5409
                     /locus_tag="PKH60_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNQ16735.1"
                     /translation="MKFALPYSQIITAEIERLLDKASNEWPAIFTFDDYFKRMSDYSV
                     RGKMVRSNLVILSWHAYQTKSTELTPEVVSLAAIQELSESGLLLQDDVMDNDVLRRGK
                     PTMHLQYRELALQDNLTVSVTSAGEFGKAWATILGDLLFFYLFRLLAQLPTQSELKLA
                     LIDLYSNELAITGSGQLADLAAGFGIVDPTQLEVGEINRQKTAHYTICLPMLAGAILA
                     GQSKEELAAITTVGETIGEIFQLTDDRLDLFSSSNVSGKTQASDVKSGKKTMLYFFTV
                     EGLQKEPDELIEFNSLYGRADLQLTEIERVQQLVKRSGGYERSEALIGELAQRARQQL
                     DKLSISDLSKQWFYSFIDFLTTREH"
     gene            5410..6762
                     /gene="phrB"
                     /locus_tag="PKH60_01395"
     CDS             5410..6762
                     /gene="phrB"
                     /locus_tag="PKH60_01395"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNQ16736.1"
                     /translation="MKFDLRRVRKLNQISQKPGRVVYWMSRDQRIEDNWAFWYGLQQA
                     TNLNQQLEIVFVLAKKFEGATLRQYQFMIDGLQILSSQANSLGIPFTIEFGLPSDVFK
                     RLHAEDSIGLLVTDFSPLKISRQWKQAVAELPFALHEVDAHNIIPPWVTSTKQEHSAR
                     TIRSKINRLLPEWSVDFPKISVQHAGLTKSPLIDWPKVRRSFVVDTSVSPITWLQAGI
                     DAAENQLSNFVANGLEQYSDQRNDPGFNGLSNLSPYLHFGQLSAQRVALRIRNSRHTH
                     SSSAEKFLEELIVRRELAENFCFYNQNYDSTESYPGWALHTLREHEQDERLHLYTQEQ
                     LEMAQTSDPLWNLAQQELRLFGKMHGYMRMYWAKQLLRWTKSVEEAQKIALYLNDRYE
                     LDGRDPNGYAGIAWSLGGVHDRPWPAQPIFGKVRSMSYESTRKKITQASYAKRINNFI
                     "
     gene            6776..7603
                     /locus_tag="PKH60_01400"
     CDS             6776..7603
                     /locus_tag="PKH60_01400"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HNQ16737.1"
                     /translation="MSKTNTYRDITLIKLGGSIVTDKSKNQHYRQEMVRQLGKELAEY
                     QQQTNEIVFIGHGQGSYAHPIVKKYADHFTSKDAFQAHKMVEMLRVISKLHEQILDDL
                     IGCGLPAVSYRFSQQASIHTDGQILMNLDLLEHLFELQTVPLTTGDIVVDANKGNAVL
                     STEKIFFKLINHLQQSSKFRVKRVAYVTEVEGVLDHDRKLIAHIGASQKLKDTLFYSN
                     TAQSDVTGAMKHKVESAQAVARQGIPVAIFSPLIENNLSDYLNEKSWRGTLISANRE"
     gene            complement(7581..8954)
                     /locus_tag="PKH60_01405"
     CDS             complement(7581..8954)
                     /locus_tag="PKH60_01405"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HNQ16738.1"
                     /translation="MFETRLPQPPTLRKTLGPSFILLGLSMGSGELLLWPYLAATYGL
                     GLLWGAVIGLTLQFVLNTEAMRYTLAWGESVFVGFQKLHPALPIWFIFSTLIPWSLPG
                     FSSATAQILHTLFPPISETFFAIALLLLTGLILSSGSSLYKTMERFQKFILFFGLSFI
                     FFITLLMTRWQDWVVASKGLVGIGENWWLLPPGLALATFLRAFAYSGAGGNLNLAQSY
                     YIKEKGFGMGKYMGKISALFSSKKQRVDLFGQHFAHTKDNARLWKHWWQLINREHMIV
                     FWGLGLLSIIVLSTLAYATSFGQTTGTGLEFLYFQASTIGKQTLPIFHPIFLLVGASM
                     LFASQTGVLESSSRIISENSILATHFKHKKVALASWFYIALWTQIGLGIVTYLIGFRQ
                     PLALITLAAILNAIAMMTSFALLFLLNRQYLLRQYQPHRNRKIILIVATVFFGFFLVI
                     TILDLLK"
     gene            9030..9737
                     /locus_tag="PKH60_01410"
     CDS             9030..9737
                     /locus_tag="PKH60_01410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNQ16739.1"
                     /translation="MKKEPYVILITGGSDGLGKVIAQHLNTSHKVVILSRNQEKLEKT
                     AAEIGCQWVQADVTQPSEVAEAVREVLTWHPQIDILINCAGRLIDGLLENYEPYEIEQ
                     VIQVNILGVMYMTRAVLPRMKLLGKGKIITIGSQAGLRGRKSRTVYTATKWAVRGFSA
                     SLQEEVAQNGISVSVINPGLMQTDLLKKAGVTGESLETGLHLDSVAALVKLVVESPES
                     ITIPELGVKTLADVHGW"
     gene            complement(9706..10482)
                     /gene="prmC"
                     /locus_tag="PKH60_01415"
     CDS             complement(9706..10482)
                     /gene="prmC"
                     /locus_tag="PKH60_01415"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNQ16740.1"
                     /translation="MQAQKNIRVWNAYEKTQLSRYKINIDSLPDDTTSPVEYLTGWVD
                     FCSYTFKVSPDTLIPRPESEELIQRALEWISKQEIGKICRVIDVGTGCGAIALSLAQM
                     VKHPITITASEISESALAIAKQNAKLFNQTSITWVVADLLSFLNPNEKVDLIIANLPY
                     IPHERIDYLDASVKNFEPHIALDGGESGLTLIASLLNQAKNHLSEDGAILLEIDYTHA
                     DELRQQFCADWRIVTWQSQISLCTFAQLWPITNHEHQPMS"
     gene            10541..11296
                     /locus_tag="PKH60_01420"
     CDS             10541..11296
                     /locus_tag="PKH60_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNQ16741.1"
                     /translation="MSSQSSTSPLGSQNTLGMVVVALVLFVAGFFLGQLWQENKMLKS
                     GIPTAAAPAVQDGAAEAPSGPTKETLGLMPKVTKDDHILGASNPKVTLVEYSDFECPF
                     CNRFHPTVKQIIEKYGDEVALVYRHFPLSFHPFAQKSAEASECVAKQKGSEGFWKYAD
                     YIFGEQTKGTAMSDDLILKSAQEAGVNVTAFQTCLDSGEMKEKVTENQTTGGAAGISG
                     TPGTIIVTQDGPQELIPGALPFESVEPMIQKYL"
     gene            complement(11366..11662)
                     /locus_tag="PKH60_01425"
     CDS             complement(11366..11662)
                     /locus_tag="PKH60_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16742.1"
                     /translation="MGDNSSAIKTIAILVLLLIALAIAAIFSYNPVPEFNIGGGVATP
                     SATQVPTCESDDPESVNTCCNEWASNNQVNVVECVGSWSWTQLGGCTFNCSIEM"
     gene            11725..12156
                     /locus_tag="PKH60_01430"
     CDS             11725..12156
                     /locus_tag="PKH60_01430"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNQ16743.1"
                     /translation="MSLKAALYEDMKTAMKSRDAQRLETIRFAISVIKNVEIDHGEQS
                     DAQVESIIRKQVKQMREAIDQFKQGNRLDLVESEEAKVTILEEYLPKQLDENEVEVLI
                     QSVISETENPNFGSVMPKAMQKLAGKADGKIVSEIVRKLLS"
     gene            12153..12797
                     /locus_tag="PKH60_01435"
     CDS             12153..12797
                     /locus_tag="PKH60_01435"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNQ16744.1"
                     /translation="MKPTTIGQALQIERQNKRVTLERLAEITNIRLDYLQALENNDFG
                     QLPTAAYVKGFIRAYAQHLSIDAKPFIALLRRDYKETNKGNLIPREFIKSYRTRKIVW
                     RPITWLSIGLVLFIGVIFCYLGIQWFRATRPPSLVITSPVSRSIVSEEVMVEGYSDSD
                     AVITVNDQPVALQPNGSFQTTIRFPVDGVGIISVIATDRRGKSTEEMLTVTVQR"
     gene            12821..13111
                     /locus_tag="PKH60_01440"
     CDS             12821..13111
                     /locus_tag="PKH60_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16745.1"
                     /translation="MENLAFVASVSLVVIAAVVVAVGIYLILLMAELRKVVHKLNFSL
                     ETTGSSIAQFIASLQHAGSYAMSFQAGVKTLESLAQWIRERTSKEDKTDKKK"
     gene            13142..15061
                     /locus_tag="PKH60_01445"
     CDS             13142..15061
                     /locus_tag="PKH60_01445"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNQ16746.1"
                     /translation="MPLSLDQLRSKYLNFFSQKQHALVPSAPLVPENDATTLFISSGM
                     QPLLEYFLGAVHPLGSRVVNSQKCFRSQDIEEVGDNRHTTFFEMLGNWSFGDYFKTEQ
                     IQFVFEFLTSDLNLDPNRLYVTVFGGSAELNIEKDEEAIVAWQKQFSAHNVDSQVVLN
                     PAKTGLGEGRICVYQWENWWSRAGKPSAMPVGEPGGPDSEVFYDFGIDRHFHENSKWK
                     DLPCHLNCDCGRFMEIGNSVFMQYMKQKDGSFTELPKKNVDFGGGLERILAALHNEPD
                     VFALPIFEPVIKKLEQLSGKKYQEAAQHTKAFRVIVDHLRAAVMLISDGVNPSNKDQG
                     YVVRRLIRRAVRYAHVLGIEDLFLAQLVSGITNLYQDQYPELSLRQKDIEQSLNQEEG
                     KFRRTLIKGEQQLKKMLTEKNSNKLSNISAQDAFYLYESFGFPLELTQEIISEAYPED
                     LLVTSDDFSQAFQKLRQQHSTSSRSASAGQFKGGLQNSSQVTTRYHTATHLLHASLRK
                     HLGNQVQQKGSHITDQRLRFDFSYDQPIDQESLTVVVDQVNQWVQADLPVHCQEMLKS
                     EALKQGALAFFVEKYPEKVTVYTIGPDPSKDWISKELCGGPHVQQTSQIGTLEVIKEQ
                     SIGAGIRRIYLRFVE"
     gene            15088..17226
                     /locus_tag="PKH60_01450"
     CDS             15088..17226
                     /locus_tag="PKH60_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16747.1"
                     /translation="MALPWLKAQTNSETPIKGTLCILLGEQTVSAALTYLTPTETRVS
                     TISLQRQFKDIESFVAATDECLRDLGEESNQINQAVFCLSEHWVDAHDVLPQKVAWIE
                     QVLTELSLQALGFIGLIDGFTSLLLKQTSKATQLVFFIFPHTITLSVLVGTHVITTLS
                     AGRSTEIADDFTELLARIKSRLTENELTSLTVTLASPDLSDGQLDILQQSLLEYDWQK
                     IVPEAALPQLTLIKQEELYRHLFHQTAEVLRSKQLGAQWHSDPLVQASKSAFGQEVAD
                     SVAASMPIQSELISPSLSKADSEAEIQDSEQGLSRRKKGLNMGFPLFLSGKRKKRLLF
                     AGLGFFFGVVVLGIIGAIYLRQYAELLITIQPKRETITKDVVVALSEGSLKITDDYSL
                     PIETVQENVEVTAAKVTTGTKTIGEKAKGRVKVINKTEQPKLLESGTTITSGTGLVFL
                     LDEAITVPAATVSATSTGEVKSYGSLEVNATAKDIGDKSNIGSGQDLKVASFSDSSYS
                     AQSIQAFTGGSSKDVAVVTEKDVYDLRSDLKKQAAQEAQESLESLKTEGKRVAPPHEI
                     SISKEQASAKVGDEAEEISLTITATAKAFAYDSQDTRNLAQAILANSIPSGTILDKTE
                     PQVLTKFSEASSSAKTQLELNISAKVVSQVNTNQIAQTLVGRPVAAINQHAVDLPGVD
                     AVTAEWIPKFALWLNQDVPKVERIRVYVTE"
     gene            17213..18193
                     /locus_tag="PKH60_01455"
     CDS             17213..18193
                     /locus_tag="PKH60_01455"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HNQ16748.1"
                     /translation="MLQNKSSSSHQISEKAQSELITLARQALLSGMAYEEVQAKIARF
                     ATRMTVSEVVEAKNIIERPRTIRERLPKPVRLGALIIPLLLLTTGVYLVGSAVVPILG
                     YYVSDESNVLQRELTSPIPRYQVMDITPLVIIPSPNVFVASEEKQVAPEVVSDTLDFT
                     NLANWFGGSAPELVEKSPDGGPVKDYTITIPSLKIEGANVEVGGTDLGKSLIAYPGTA
                     LPGEFGAPVIFGHSVLRQFYNPSEKNPNRYNSIFSTIMTLKVGVDKIYIEADGVKYTY
                     LVQQKTEVKPEDVFILTQQYDAKRLKLVTCVPEGTYLRRGVIIAQLVEEH"
     gene            18224..18427
                     /locus_tag="PKH60_01460"
     CDS             18224..18427
                     /locus_tag="PKH60_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16749.1"
                     /translation="MIEDEATQEIAAVMRLITAAERNEDRFTGDIAFAKAVRLLMTGR
                     DQIPDSTFKIIGLDRVQDIPNNE"
     gene            18420..18845
                     /gene="ruvX"
                     /locus_tag="PKH60_01465"
     CDS             18420..18845
                     /gene="ruvX"
                     /locus_tag="PKH60_01465"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNQ16750.1"
                     /translation="MNNYTLEQSQFPPVTLGIDYGRKRIGLALSYGSLAEPLTIIEND
                     ERVLEKLHFVCVEHRVAQLVVGISEREMAVESHQFGQLLTSQLTLPVKYIDENFTSRA
                     VQEKMQWLGGKQRRKEPIDHLAAAEILQEWLDQQQSPSE"
     gene            18972..19856
                     /gene="mltG"
                     /locus_tag="PKH60_01470"
     CDS             18972..19856
                     /gene="mltG"
                     /locus_tag="PKH60_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNQ16751.1"
                     /translation="MDEQNAEEVRFVITKGESITAIGEKLTTAKLIRHPLLFRFAVQQ
                     QQLGQKIQAGSFLLSPAQNLWQIAETLTQGTEDVWVTIPEGYRVEEIAQTFSNAGLVE
                     FNVDDFIAQAKDQEGYLFPDTYLVPKLITAPEVIKLLNRTFDQKVRQGLADEIANSQH
                     SLEDAIIMASLVQREAGKDEDEMKQVAGVLWNRIAINQGLGVDATLQYIDGFDEKTGK
                     WWSNPDVRTKESTSPYNTYKYAGLPPAPIANPGVAAIRSALDPLKTDNYYYLHAEGKI
                     YYAKDLAGHSSNIDRYLR"
     gene            20045..>21009
                     /locus_tag="PKH60_01475"
     CDS             20045..>21009
                     /locus_tag="PKH60_01475"
                     /inference="COORDINATES: protein motif:HMM:NF016448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNQ16752.1"
                     /translation="MSDNTRQNWGKHHVVVPELDLVGLQKQSYQEFLENGIATALREV
                     TGEKGIEDYTGKSWALKFGGYRFGEPKYSATEAKRKGVTYDMPLYVQAELLNKKTGKV
                     QEQEVFLGDIPKMTTIGTFVINGIERAVVTQLVRSPGVFFTGETDASTRKDLYFAELR
                     PKRGSWLEITVGKRDVIAVKIDRRRKMPVTVLLRALGYGTDDDIKALFADEIGFDEFK
                     LLQTTLEKDTTQSQPEALLELYEKMRPGEPAVIDTAQEYVHQLFFDSRRYDLGLVGRY
                     KLNRRLKLSVDENTTVLTPEDVVATVKYLIGLQNGRGKTDDIDHL"
BASE COUNT         6537 a         3997 c         4594 g         5881 t
ORIGIN      
        1 agaaaatttc cataactttg ccaacgtgta tcaattcagg ccaggatttt tttcctttga
       61 ggttatcata ttgaaatttt ctactatact atcgaacaga tgaaaacata tgtagtatct
      121 gccaaagtga aacatggcga ccagcatggt cgctcaattg ggcatccaac tatcaaccta
      181 gattcgactt tgtggcccgc tgatttacag ccaggtgtat atgctagtaa agtaacgctc
      241 ggagaaaagc agtaccttgg agcgctttac tttgggccac gaagcatcaa ggcagaaact
      301 aaaaatatac tcgagattac cctatttgag ttctctgaca cgatttatgg agaggtggtt
      361 tcagttttag tcggtgattt tgttcgcgaa cctatcgagt atcaacctgg caaacagaat
      421 gaaaatgctc tgaaagaaca aatcgccgat gacgtttcaa aagttatgca attgggaaga
      481 ttgcggtggc cccaacttgc tatactatag gagattatgc cggataaata ctcagctctt
      541 tggttatcac attcaagtat tagagatttt caacagtgtc caagatcgta ttatctgaat
      601 aatatgtatc gcgatcctca aaccaataaa aaaattcaac tagtttctcc tccgctatca
      661 ttgggtagtg ctgtgcatca aatcattgag catctctcag atttgcccgt agataaacga
      721 tttgaaactt ccttggtgga attatttgac aagatttggc ccaactttac tggtctcaag
      781 ggtggttttt ctgatcaaac cactgaggaa agatataagg agcggggcaa ggcgatgttg
      841 agaacagtga tgcaacatcc aggtccacta gcgaacaaag ctatcaaggt tcaagaagat
      901 ctgccccaat attggctttc tgaatcggat gaaatgattt tatgtggcaa aattgattgg
      961 ctagaatata ttccggaaac agactcggtg catattattg attttaaaac tgggaaaaat
     1021 aatgaggaag gttccttgca attgccaatt tatcttttat tggttagcaa tgtccaaaaa
     1081 cgaaaagtgg cgggcgccag ttactggtat ctggaaagtg gtgccaattt gaccgagcag
     1141 acattaccaa ctgctgaagc agcgtacgat caggttatgc tggtggctaa gcaaataaag
     1201 ttggctagaa agcttgacag atttaagtgt cctcagggag aaattggctg caaattttgt
     1261 agatcattag aaaaaattgt atctggggaa gcgatttttg tggggcaagg tgagtatggc
     1321 aaaaactgct atgttttgcc caaagatcaa atgcaagaag caaaattgtt atagagaggg
     1381 tgtatggcaa agatcatagt gattggagcg ggaattggcg gaatatctgc ggccgctcac
     1441 ttggccaaag ccgggcatag agtaacagtg ttggaaaaaa acgatcagcc tggcggccgc
     1501 gctgagatat ttcaagaaaa aggctacact tttgatcgtg ggccctcttg gtatctgatg
     1561 ccagaggttt tcgataaatg gtttgccgat cttggatttg atagggaaga ttattatcaa
     1621 attattacat taaatcccca gtataaagtt tactatcagg ataagactca gctggaaatt
     1681 ggtatagata gagagaaaaa cctccataag atagctagat tgcaaccagg aagcttgcca
     1741 aaattacgcg catatttacg acaggcacag acaaaatatg atctggcaat ggaaacattt
     1801 ttgtataaaa atgctgactc gcctttggat tttttgcagt attggcgcct gctgccgcag
     1861 gcgctccagc tcaatctgtt tggtaccatg caacactaca ttgagcgcta tattacggaa
     1921 ccgaagctcc aacaactact tatgtataac ctggtttttc tgggttgttc tccatacaat
     1981 gctccggcac ttttcagcct aatggctcac gttgatctga atgtgggagt ctattatcct
     2041 gctgggggtt ttttaaatat ggtggcaagt atggtttcaa ttgccaaaca attgggagtt
     2101 cgatttgagt acaaccaacc aatcaaaaaa ataaacacaa catcgggttt agttacctcg
     2161 ctggaaacag atataaaaac ctggcaggct gactatgttg tttctaacgc agattatgtt
     2221 cacaccgaag atctcctgag cgaccaaact atgcgtcagc actcagatgg gtattggaaa
     2281 cgaaagatta atggtccttc cgcttttctt ttgtacttag gcattaacag aaaaattcct
     2341 gaattgatgc accacacgct atactttacc gaggattggc aaaaacattt ttcagatgtt
     2401 ttcaatcata agcagtggcc acaaaatcca tcaatttata tcaaccggcc aactgccacg
     2461 gatgcttccc ttgcccctaa agggcacgat acactcatga tcctcgtacc tgtagcatct
     2521 ggacttgcag actcaaagag cttgcgcgaa tcctatggcg actatattat taagtacatt
     2581 gaaaaaaatc tgaacattga gtttcattct gcaataaccg taaaaaaaat cgtgacaatt
     2641 tcggactttg agaaacgtta caacagtttt gagggcaatg cctttggggg attagcgcac
     2701 accttgtttc agagttccat ttggagacca agtaatcgtc ataaaaaact ttcaaacttg
     2761 tttttcactg gtgctggaac agtgcctgga attggtttac caacttcact catttcaggt
     2821 tatttagtga atgacagaat tgctaaattg aaatgacgta tctttacatc ctgattagca
     2881 ttattatttt tccgtttatt attggtttaa cttcaggttt tagattttat tttaatcgaa
     2941 aactagtaaa aacacttttt ttgaccgcta caccattttt aatttgggac tggtgggcta
     3001 ctacccaagg acactggtcg tttaacccca cacatattat tggtgttaca tttttacatc
     3061 tacctatcga agaaatcgcc ttcttttttg ttgctccttt tgcgtgtctc tttatctatg
     3121 cggctggtga aaaatatttg caaagaagat atatatcact gccttttttc ttttgggtgc
     3181 tgataactgg tgcatccatg ctgttcttgg ttgttggttt ccctaagccg tatagcactc
     3241 ttgtttttgg attaagcact ctacttttat tgtacagtta tcaaagattt cgatctcaat
     3301 ttactacccc catcctcatc tattttattt ccagcttcat tttctttctc ctctttaatt
     3361 cgcttttaac catggtaccc gttgtgagtt atgctccgtg ggcaattctt aacttgagag
     3421 ttggtacaat accaattgaa gatttttggt acaatttctt gcttttatcg tttacactaa
     3481 ttatttatag agttatgtca aacaagcaca ccaatgcgca agctgtcttt cagaagggaa
     3541 gtacaacgta ctatactgcg agtcagtttt tttcacccaa aattagagaa aaagttacaa
     3601 ctctatatgc ctttgtgaga ttggcagata actacgttga tcaattacca gaaaagaaaa
     3661 aagaactaga agctttcttt gcagaatatc acaacgagat tcgttcaccc ggaaaggctc
     3721 gtcaagtaat ttccaacttt gtgtcactga tgaaaaatac gggaattaag cccgcttggg
     3781 ttgaagcctt tcttgattca atgatccaag atattactaa gaaaacatat gcaaactttg
     3841 gcgatttatc taagtatatc tatggatctg cagaggtcat cggattaatg atgtctcgca
     3901 tatttggctt atcgtccgat catgatcctg ctgcagctaa gcttggtaaa gctatgcaat
     3961 attgcaatat gcttcatgat attgatgagg atttaagctt gggaagaatt tatctgcccc
     4021 tatcagtgtt acagaaatac aagcttgaac ccttttcaaa ggtaatggcg caaaaaaaac
     4081 caaagcagtt tcggttgctt atgcaacaag aactatctcg atgcttgaag tggtttgacg
     4141 aagcagagaa agacatatca tttattcctc gaagagaaag agtggcggtc aaaacagctt
     4201 cggcaatgta tcgatggaca gcggagcaat tgtataaaaa tccacaattg gtgtttcaaa
     4261 aaaagttaaa accaagtaag ttgcgagtga tactcactgc tatttcgttc agtattaaag
     4321 gctaacgtta tgaaattcgc tctaccttac tcacaaataa ttactgcaga aattgagcga
     4381 ttattagata aagcaagtaa tgaatggccg gcgattttta cttttgatga ctattttaag
     4441 cgaatgagtg attattcagt gcgaggaaaa atggttagaa gtaatctggt gatactgagt
     4501 tggcatgcgt atcaaacaaa atcaaccgaa ctgactccag aggtagttag cttggcggca
     4561 attcaagaac tgtccgaaag tggattgtta ctccaggacg atgtgatgga taatgatgtc
     4621 ttgcgtcgag gtaaacccac catgcacttg cagtatcgag aattggcatt acaagacaat
     4681 ttaacagtat ctgtaacgag tgctggagaa tttggaaaag catgggcaac tatattgggg
     4741 gatctgttgt ttttctattt atttaggtta cttgcacaat tgccaactca atcagagtta
     4801 aaattagcgc ttatagattt atatagcaat gagttggcta ttacaggatc tggacagttg
     4861 gctgacctag ctgccggatt tggaattgta gatccaactc agctggaagt gggtgaaata
     4921 aatcgccaaa aaaccgctca ctatacaatt tgtctaccaa tgcttgcggg agcaattttg
     4981 gcaggtcaat cgaaagaaga acttgctgca atcacaacgg tgggagaaac cattggagaa
     5041 atatttcaac tgacagatga tcgccttgat ttattctctt cttcaaacgt ttctggaaaa
     5101 acacaggcaa gtgatgttaa aagcggtaaa aaaaccatgc tgtatttctt tactgtcgaa
     5161 ggattgcaaa aagaacccga tgaattaatt gaatttaatt cgttatacgg tagggcagat
     5221 ttacaactaa cagaaatcga gcgtgtccaa caattagtaa aaagatctgg tggatatgag
     5281 cggagcgaag cacttattgg cgagctcgct cagcgagctc gccagcaact tgataaatta
     5341 tcaatttcag atttatccaa acagtggttt tattcattta tagacttttt aactacgcgg
     5401 gaacattaga tgaaatttga tttgcggaga gtaagaaaac tgaatcagat ttctcaaaaa
     5461 cctggacgcg tggtgtactg gatgtcacga gatcagcgaa tcgaagacaa ttgggctttc
     5521 tggtatggct tacaacaagc gacaaatctg aatcagcaat tagaaatagt ttttgtgctt
     5581 gccaagaaat ttgagggagc aacgctgcgt caatatcagt ttatgattga cggcttacag
     5641 atattgagta gtcaagctaa ctcgcttggt attcctttca ccatcgaatt tggtttgcca
     5701 agcgatgttt ttaaacgtct gcacgctgaa gattcaatcg gtttgttggt cacggatttt
     5761 tctccactta aaatttcacg acaatggaag caagccgtgg cggaacttcc attcgcgctc
     5821 catgaagttg atgctcacaa tattattccc ccatgggtta ccagtaccaa gcaagaacat
     5881 tcagctcgca caattagatc gaaaatcaat cgcttactac ctgagtggtc agtagatttt
     5941 ccaaaaattt cggtacaaca tgccggttta actaaatctc cgctgataga ttggccaaaa
     6001 gtccgtcgct cgtttgtagt agataccagc gtgtctccaa ttacatggtt gcaagccggg
     6061 attgacgcag cagaaaatca actaagcaat ttcgttgcaa atggattgga gcagtattca
     6121 gatcagcgca acgatccagg tttcaatggt ttatcaaatc tttcgccata cttacatttt
     6181 ggacaacttt cagcgcagcg cgtggcgctg cgcattcgta acagccgtca cactcactcc
     6241 agcagcgcag aaaaattttt ggaagaactc atcgtacgta gagaattggc agaaaacttt
     6301 tgtttctaca atcaaaacta tgattcaact gaaagttatc caggctgggc gcttcatact
     6361 ctgagagaac atgagcaaga tgagcgattg catctatata ctcaggagca attagagatg
     6421 gctcaaacat cagatccact gtggaatctt gcacaacaag agctaagact ttttggcaaa
     6481 atgcatggat atatgagaat gtattgggca aaacaattgt tgcgctggac caaatcagtc
     6541 gaagaagctc agaaaatcgc actatatctt aatgaccggt atgaacttga tggcagggat
     6601 cccaatgggt atgcgggcat tgcttggtcg cttggtggtg tccatgatcg accttggcca
     6661 gcacaaccaa tttttggtaa ggtacggagt atgagctatg aaagtacaag aaaaaaaatt
     6721 actcaagcgt catatgcgaa gcgaataaac aacttcatct gatagaatcg actttatgtc
     6781 aaaaactaat acttacagag acataaccct catcaaactc gggggctcaa tagttactga
     6841 taagtccaaa aatcagcact accggcaaga gatggttcga cagttgggaa aagaactagc
     6901 cgaatatcaa caacaaacaa atgagatagt attcatcggt catgggcagg gaagttatgc
     6961 tcatccaatt gtgaaaaaat atgcagatca ttttacctcg aaggacgctt ttcaagcgca
     7021 taaaatggta gaaatgttga gagttatctc gaaactgcat gaacaaattc ttgatgattt
     7081 gattgggtgc ggtttaccag cagtaagtta tcgatttagt cagcaagcgt ctatacatac
     7141 agatggtcaa attctaatga acttagatct tcttgaacat ctgtttgaat tgcaaacggt
     7201 tccgctaaca acgggagata ttgtggtaga tgctaataaa ggcaacgccg tactttctac
     7261 agaaaaaata ttttttaaac taatcaatca tttacaacaa tctagtaaat ttcgagtaaa
     7321 aagagttgcg tatgtcactg aagttgaagg tgtactagac catgatagaa aactgattgc
     7381 ccacattggt gcctcccaga aattgaagga tactttattt tattccaaca ctgctcaaag
     7441 tgatgtcacg ggagcaatga agcataaagt tgagtccgcc caagcagtgg cgcgtcaagg
     7501 aattccggta gccatttttt caccactcat agaaaacaac ctgtcagatt acctgaatga
     7561 aaagtcctgg cgcggaacac ttatttcagc aaatcgagaa tagtaatcac caaaaagaat
     7621 ccaaagaaga ctgtggcgac aattaatata atttttctat ttctgtgagg ttgatattgg
     7681 cgaagtaaat attgtctatt gagtaaaaac aataaagcaa aggatgtcat cattgcaatt
     7741 gcatttaaaa ttgcagctag ggtaatcagt gctaaaggtt gacggaaacc aattaaatag
     7801 gttacgattc cgagcccaat ttgagtccaa agagctatgt agaaccaact tgccaacgcc
     7861 actttcttat gtttaaagtg ggtagccaaa atactatttt cggagataat tcgagacgaa
     7921 gattcaagga cgccagtttg gcttgcaaac aacatactcg cacctactag taaaaatatt
     7981 ggatgaaaaa tagggagggt ttgcttgcca atagtactcg cttggaaata cagaaattct
     8041 aatcctgtgc cagtagtttg cccaaaggag gtagcatatg ccagtgtgga gagcacaata
     8101 attgacaaaa gacctaatcc ccaaaatact atcatgtgct ctctattaat caactgccac
     8161 caatgtttcc agagcctagc gttatctttt gtatgggcaa agtgttggcc aaagagatca
     8221 accctttgtt tttttgatga gaaaagagcg ctaattttcc ccatgtattt acccatccca
     8281 aagccttttt ctttgatgta gtaagattgg gctaagttga gatttccccc agctcctgag
     8341 taggcaaagg ctcgcaaaaa ggtggctagt gcaaggccag gaggcaataa ccaccagttt
     8401 tcaccaatcc caactaaacc cttgctagcc acaacccaat cttgccatcg agtcatgaga
     8461 agcgtgataa agaatatgaa agataggcca aaaaaaagaa tgaatttttg aaatcgctcc
     8521 atggttttgt ataaacttga tcccgatgaa aggatgaggc cagtgaggag taagagtgca
     8581 atggcaaaaa atgtttcaga aatgggcggg aacaaagtgt gcagaatttg cgctgtagct
     8641 gaagaaaatc ctggcaggga ccagggtatt agagttgaga agataaacca gatgggaagc
     8701 gctgggtgta atttttgaaa accaacaaag acactttcgc cccatgctaa cgtataacgc
     8761 atggcttctg tgtttaacac aaattgaagt gttaggccaa taaccgcacc ccagagcaaa
     8821 ccaagtccat aggtcgcagc caggtatggc cacagtaaca actctccact acccatggac
     8881 aatccaagca aaataaaaga tggtccaagc gttttcctca gtgtaggtgg ttggggtagg
     8941 cgcgtttcaa acatgattga tgaaatcagt atagcctaaa aaatggtatg attacaacaa
     9001 attgctgttt ttgtaaaaaa tcacaatcta tgaaaaaaga accgtatgta attctcataa
     9061 cggggggatc ggatggtctg ggcaaagtga ttgctcagca tctcaatact tcacacaaag
     9121 tcgttatttt gtcaaggaat caggaaaaat tagagaaaac tgctgcagaa attggttgtc
     9181 agtgggtaca agcagatgta actcagcctt cagaggtagc cgaagcggta agagaagtgc
     9241 tgacttggca tcctcaaatt gatattctga ttaattgtgc tggtcgatta atcgatgggt
     9301 tattggaaaa ctacgaacca tatgaaattg aacaagtaat ccaagtgaat atcctcggtg
     9361 taatgtatat gactcgagct gttttacccc gtatgaagtt gttgggaaaa gggaaaatta
     9421 ttacgattgg atcccaggct gggcttagag ggagaaaatc tcgaaccgta tatactgcaa
     9481 caaaatgggc agtccgtgga ttctcagcgt ctctgcagga agaagtggcg caaaacggca
     9541 tcagcgtttc agttatcaat ccaggcctaa tgcaaaccga tttattaaaa aaagccggag
     9601 taaccggaga atcgttagaa actggcttgc acctcgattc agttgcagcg cttgttaagt
     9661 tggtagttga gagtccagaa tcaattacta ttccagaact cggggttaag acattggctg
     9721 atgttcatgg ttggtgatag gccaaagttg agcaaaggta caaagactaa tttgactctg
     9781 ccaagtgacg attctccaat cagcacaaaa ttgctgtcga agttcatccg catgtgtgta
     9841 atctatctct agtaaaattg cgccatcctc ggaaagatgg ttttttgctt gattgagtaa
     9901 gctggcgatc aatgttaatc cagactcacc accatctaga gctatgtgtg gctcaaagtt
     9961 tttcactgat gcatccaggt agtcaattct ttcatgcggg atatatggta ggttggcgat
    10021 aataagatca actttttcgt tgggattaag aaatgaaagc agatctgcaa ccacccaggt
    10081 gattgatgtc tggttgaaca atttggcatt ttgtttggca attgctaatg ccgactcaga
    10141 tatttcactc gcggtgatag tgattgggtg ttttaccatc tgagcaaggc tcagtgcaat
    10201 tgctccacaa ccagtaccaa catcaattac ccgacagatc ttaccgattt cttgctttga
    10261 gatccattcg agtgctcgtt gtattaactc ctcagattct ggtcttggaa taagagtgtc
    10321 gggtgaaact ttaaaggtat atgagcaaaa atcaacccag ccggttaggt attcaactgg
    10381 cgaagtcgta tcatccggaa gtgaatcaat gttgatttta taacgactga gttgggtttt
    10441 ctcataggcg ttccagactc gaatgttttt ttgtgcttgc attttagctg tttttcgtta
    10501 gagtgtatga attattgtaa tacgagcata aggatacact atgtcatcac aatcaagtac
    10561 tagcccgttg ggcagtcaaa atacattggg aatggtagta gttgctttag ttttatttgt
    10621 tgctggcttt tttcttggac aactttggca agaaaataaa atgctaaaaa gtggcatccc
    10681 gactgcagca gctcccgcag tccaggatgg tgcagcagaa gccccatctg gtccaactaa
    10741 agaaacactt ggattgatgc ctaaagtaac gaaagatgat catattttgg gagctagcaa
    10801 tccaaaagtg actttggtgg aatattctga ttttgaatgt ccattttgta accgatttca
    10861 cccaactgta aaacaaatta ttgaaaaata tggagatgaa gttgcattag tctatcgtca
    10921 ttttccactc agttttcatc catttgccca gaaatcagct gaagcatcag aatgtgttgc
    10981 caaacaaaaa ggctcagagg gattttggaa atatgcagac tatatttttg gtgaacaaac
    11041 caaaggaact gcgatgagtg atgacttaat tttgaaatct gctcaagagg caggagtgaa
    11101 tgtgactgcc tttcaaactt gtttagatag tggtgaaatg aaagagaagg ttacggagaa
    11161 tcaaaccact ggaggagcgg caggaatttc aggtacacca ggtacaatca tcgtgactca
    11221 ggatggtcct caggaattaa ttcctggagc attgccattc gaatctgttg aaccaatgat
    11281 ccagaaatat ctgtaattat tttgcaggaa caagatgtaa gaaaacccca gctgaaaagc
    11341 tggggttttt aatttgaaat ttaatttaca tttctatgga gcaattaaaa gtgcaacctc
    11401 caagttgcgt ccaactccaa ctcccaacac actcaaccac attaacttga ttgttgctag
    11461 cccattcatt acagcaagtg tttacagatt caggatcatc actttcacaa gttgggactt
    11521 gagttgcgga tggggttgcg actccaccac caatattaaa ttctggcact gggttgtaag
    11581 aaaagattgc ggctattgcc agtgcaataa gcaataacac taatatagca attgttttta
    11641 tagctgaaga attatctccc atgccttaag tatacacgaa tggtgtaaaa tcaaaaccgt
    11701 atgtaggtaa tcagaaaagg atgtatgtca ttaaaagctg cgttatatga agacatgaaa
    11761 acggcgatga aatcgagaga tgctcaaaga cttgagacaa tcagatttgc aatttcagtg
    11821 atcaagaatg tggaaattga tcacggagaa caaagcgatg cacaggttga gagtattatt
    11881 agaaaacagg ttaaacaaat gagagaagcg atcgaccaat ttaaacaggg caatcgtttg
    11941 gatttagtcg agtctgaaga agcaaaagtg acaattcttg aggaatattt acccaaacag
    12001 cttgatgaaa atgaggttga agttttaatt cagtcagtaa tttctgaaac agaaaatccc
    12061 aattttggta gtgtgatgcc aaaggccatg caaaaacttg cgggtaaagc tgatggaaaa
    12121 attgtttctg aaatagtacg aaaattgctc tcatgaaacc tacaacaatt ggacaggcgc
    12181 ttcagattga aagacaaaat aagcgcgtca ctttggagcg gttggcagag attaccaaca
    12241 ttcgtctaga ttacttacaa gcactggaaa ataatgattt tggtcagttg ccaactgcag
    12301 catatgtgaa aggatttatc cgagcttacg ctcagcattt aagtattgac gccaaaccat
    12361 tcatcgcttt attacgacga gattataagg aaacaaacaa gggcaacttg attcctagag
    12421 agtttataaa gtcctataga acacggaaaa tagtatggag acccatcaca tggctttcca
    12481 ttggtttagt gctttttatt ggagtaatat tttgctactt aggtattcaa tggttccgcg
    12541 caacgaggcc tccatcattg gtgatcacgt caccggtgtc tcgttcaatc gtatcagagg
    12601 aggtaatggt tgaaggttac agcgactccg atgcagtaat tacagtaaat gatcagcccg
    12661 ttgcccttca gccaaatggg tcgtttcaaa caaccatacg ttttccggtt gatggagtag
    12721 gaatcatctc tgtaatcgcc actgatagac gggggaaaag cacagaggaa atgctaactg
    12781 tgactgtaca gcggtaattt atgtagtgta ggatggagac atggaaaatc ttgcctttgt
    12841 tgcgtcagtg tctttagtgg tgatcgcggc agttgtagtg gcggtgggaa tttatctcat
    12901 tctgctaatg gctgagctac gaaaagttgt tcataagctc aatttttcac ttgaaactac
    12961 gggttcaagc atcgctcagt ttattgcatc attgcaacac gcgggttctt atgccatgag
    13021 cttccaagcc ggagttaaga cgcttgagag tttagcgcag tggattcgag aacgtacctc
    13081 aaaagaagat aaaactgaca agaagaagta aaaattgtac aaaaactggt acaatgtggc
    13141 cgtgccacta tcacttgatc aacttcgatc caaatacctc aattttttta gccaaaaaca
    13201 gcatgcgctt gtgccctcag cccctcttgt gcccgaaaat gatgcaacta ctttgtttat
    13261 tagctctggc atgcagccac ttctcgagta ttttctaggt gctgttcatc ctctgggttc
    13321 tcgagttgtg aatagccaaa agtgttttcg gtctcaggat attgaggaag ttggtgacaa
    13381 ccgtcacaca acattctttg agatgctcgg caactggtct tttggagatt attttaaaac
    13441 tgagcagatc caatttgtgt ttgagtttct taccagcgat cttaacttgg atcccaatag
    13501 gttatacgtt acagtttttg gtggctcagc tgaactaaat atagaaaaag acgaagaagc
    13561 gatagtcgct tggcagaaac agttttctgc tcataatgtg gattcacaag tagttttgaa
    13621 tcctgcgaaa accggactgg gtgaaggcag aatttgtgtt taccaatggg aaaactggtg
    13681 gtcaagagcg gggaaaccga gtgcgatgcc agttggtgaa ccaggtgggc cagacagcga
    13741 agttttttac gattttggaa ttgatcgtca ttttcatgag aattccaaat ggaaagacct
    13801 accgtgccac cttaattgcg actgtggcag atttatggag attggtaatt ctgtatttat
    13861 gcagtatatg aagcagaaag atggttcatt tactgaatta ccaaagaaaa atgtcgattt
    13921 tggtggtggc ttggagcgaa ttttagcagc acttcataat gaaccagatg tttttgcttt
    13981 accaattttt gaaccggtga ttaaaaagct agagcaatta agtggcaaaa aataccaaga
    14041 agcagcgcaa cataccaaag cattccgagt gatcgtagat catttgcgcg cggcggtgat
    14101 gctcatctca gatggtgtga atccttccaa caaagatcag ggatatgtag ttcgtcgatt
    14161 aatccgccgc gctgttcggt acgcccacgt gctagggata gaagatctgt ttttagcgca
    14221 gctggttagt ggcataacaa atttatatca agatcaatac ccagaactca gtttgcgcca
    14281 aaaagatatt gagcaatcat taaatcaaga agaaggaaag tttagacgaa ctttgatcaa
    14341 aggcgaacag caactaaaaa aaatgcttac agaaaagaat agcaacaaac tttcgaatat
    14401 ttcagctcaa gacgctttct atttatatga atcatttgga tttccacttg agcttactca
    14461 agaaattatc tcggaagcat atccagaaga tttgttggtt acaagcgatg atttttcaca
    14521 agcatttcaa aaactacgcc aacaacactc gactagttca agatcggcct cggctggtca
    14581 attcaaagga ggtttgcaga atagctctca agttactact cgatatcaca ctgctaccca
    14641 cttgcttcat gctagtttac gcaaacactt gggtaaccaa gttcaacaaa aaggctccca
    14701 tatcacagat caacgactac gctttgattt ttcttatgat cagccgatag atcaagaatc
    14761 tttaacagta gttgtagatc aagttaacca atgggtgcag gcagatcttc cagttcactg
    14821 ccaggaaatg ttgaagtcag aagccctaaa acaaggagca ttagcatttt ttgtagaaaa
    14881 atatccagaa aaagtcacgg tatatacgat tggacctgat ccgagtaagg actggatttc
    14941 caaagaacta tgtggagggc ctcacgtgca gcagactagt caaatcggca ctttagaagt
    15001 aattaaagag caatcaattg gtgctggtat caggcgcatc tatcttagat ttgtcgagta
    15061 ggctagaatc tgctttaata gaactatatg gcgctgcctt ggctcaaagc tcaaacgaat
    15121 tctgaaaccc caattaaggg gacattatgt attttgcttg gagaacagac ggtatccgct
    15181 gccttaacgt atctcacgcc aactgaaacc cgcgtcagca ccatttcact ccaacgccag
    15241 tttaaagata ttgaatcatt tgtggccgcc acggatgagt gcttgcggga tcttggtgaa
    15301 gaatcaaatc aaatcaatca agcagtattt tgtttaagtg agcattgggt tgatgctcac
    15361 gatgtgttac cccaaaaagt tgcttggata gagcaggtgt tgacagaact ttccctgcaa
    15421 gccctaggtt ttataggttt aatcgatggt tttacctcac tcctactaaa acaaactagc
    15481 aaagctactc aattagtctt ttttattttt cctcatacta ttacattgag tgtgctagtt
    15541 ggtactcatg ttattaccac actgtcagcg ggaagatcaa ctgaaattgc agatgacttc
    15601 acggagttat tggcgcggat taaatcgcga ctgacggaga atgaacttac ctcgttgaca
    15661 gttactctag catctccaga tctttccgac ggacagctag atatattaca gcaaagtttg
    15721 ctggaatatg attggcaaaa aatagtacct gaagcagcat tacctcaact cacattgatt
    15781 aaacaagagg agctgtatcg gcacctcttt caccagactg cagaggtttt acggagtaaa
    15841 cagttgggag ctcaatggca cagcgatccc ttggtgcaag ccagcaaatc cgccttcggt
    15901 caagaagtgg cagattcagt tgctgcttcc atgcccatac aatccgaact tatttcccct
    15961 tctttgtcaa aagctgattc tgaagcagaa atccaggact cagaacaggg attgagcaga
    16021 agaaaaaaag ggcttaatat gggctttcca ctctttctta gcggtaaacg taaaaaacgc
    16081 ttgctatttg ccggattagg cttctttttt ggtgtagtgg tccttggtat tattggagcc
    16141 atctatttac gtcagtatgc ggagttatta attacgattc aaccaaagcg ggaaactatt
    16201 accaaggatg tcgttgttgc gttgtcagaa ggatcgctca agataactga tgattacagc
    16261 ttgccaattg agacagtaca ggaaaatgtt gaggtgaccg cagcaaaagt tactactggc
    16321 accaaaacca tcggagagaa agctaagggt agggttaagg tcataaataa gacagaacag
    16381 ccaaaattac tcgaaagtgg aaccaccata acctctggaa ccggtttggt ttttctactc
    16441 gatgaagcaa ttactgtacc tgcggcaaca gtgagtgcga ccagcactgg agaagttaaa
    16501 tcgtatggca gtttggaagt taacgctact gcaaaagata ttggagataa aagtaatatt
    16561 ggtagtggtc aagatttgaa agtggcctca ttttcggatt ctagctatag tgcccaaagc
    16621 attcaggcgt ttacaggagg ctcaagtaag gatgtggcgg ttgtaacaga aaaagatgtt
    16681 tatgatttgc gatcagatct aaaaaaacaa gctgctcaag aagcgcaaga aagcttggaa
    16741 tcgttgaaaa ccgaaggtaa acgagttgcc ccgccccatg aaatttcaat ctctaaagag
    16801 caagcttcag caaaagttgg tgatgaagca gaggaaattt cactgacaat tactgccaca
    16861 gccaaagcat ttgcatatga tagccaagac accaggaatt tggcgcaagc aattttggcg
    16921 aactctattc cgagtggaac tattttggat aaaacagagc ctcaagtgct aacaaaattt
    16981 tccgaagctt ctagttcggc aaaaacccaa cttgagttaa acatttctgc aaaagttgta
    17041 agtcaagtta ataccaatca aattgctcaa actctcgttg gtagaccagt tgctgcaatt
    17101 aatcaacatg ctgtagactt acctggagtc gatgcagtaa cggcagagtg gattcctaag
    17161 tttgctttgt ggctcaatca agatgtgccg aaggtagagc ggattagggt ctatgttaca
    17221 gaataaatca tcatcatcac atcaaatttc tgaaaaagcg cagtctgagc tcattacttt
    17281 ggcgcgacaa gcattgttgt ctggcatggc gtatgaagaa gtgcaggcaa aaatagctcg
    17341 atttgcaacg cgcatgactg tcagcgaagt agttgaagca aaaaatataa ttgaaagacc
    17401 gagaactatt cgtgagcgtc tgccaaaacc agttcgtttg ggggctttga ttattccctt
    17461 gttgttatta acaaccggag tctacttggt tggaagtgca gtggtcccaa ttttgggata
    17521 ctatgttagt gatgagtcaa atgttttaca acgagaatta acatcgccaa ttcctcgata
    17581 tcaagtgatg gatattactc cattagtaat aattccctct ccaaatgtat ttgtagcttc
    17641 agaggaaaaa caagtagctc cagaagttgt cagcgatacg cttgatttta ctaatttggc
    17701 gaactggttt ggcggctcag ctcctgagtt ggtagaaaaa tcacctgatg gaggaccagt
    17761 caaagactac accatcacca ttccgagttt aaagatagaa ggcgcaaatg ttgaggtggg
    17821 aggtaccgat ttaggaaaga gtctcattgc gtatcctggt actgccttgc ctggagaatt
    17881 tggtgcccca gtcatatttg gtcattcagt attacgccaa ttttacaatc catcagaaaa
    17941 aaatcccaat cgttacaact cgatttttag tacgattatg actttgaaag ttggggtgga
    18001 taaaatttat atcgaagctg acggtgtgaa gtacacctac ctagttcaac aaaaaaccga
    18061 agttaaacca gaggatgttt ttatcttgac gcaacaatat gatgccaaac ggctcaagtt
    18121 agttacttgc gttccagagg gaacgtattt gagaagaggc gtaatcatcg cccagctggt
    18181 agaggagcat taagcgttga ttcgagtata ataatgaata attatgatag aagatgaggc
    18241 aacacaagaa attgcagctg ttatgagact tattacagct gctgaaagaa atgaagacag
    18301 atttacagga gatatcgcat ttgcaaaagc tgtgcgatta ctgatgactg gcagagacca
    18361 aatacccgat tctactttta aaattattgg actagatcgt gtacaggata ttccgaataa
    18421 tgaataatta tactttggag caatctcaat ttccccccgt cacccttggt atcgactatg
    18481 gccgcaagcg cataggtctt gctttgagtt atggatccct tgccgagcca cttacaatca
    18541 ttgagaatga tgaacgagta ttagagaaat tacattttgt ttgtgtcgag catagggttg
    18601 ctcaattagt agtgggtatt tctgagcgag aaatggcagt tgaatctcac caatttggcc
    18661 aattactaac cagtcagcta acactccctg ttaagtatat cgacgaaaat tttacttcaa
    18721 gagcggtgca agaaaaaatg caatggttag gaggaaagca gcgacgtaag gaaccaatcg
    18781 atcatctggc tgccgcagag atacttcagg agtggcttga tcaacagcaa tcgccaagtg
    18841 agtaaaaatc ccattagcac atgcggtata ctgaacgttc aatgaaaaaa ttgctcgtat
    18901 taattccaat tatttgcgcc atagtttctc tttttagtct gtttgctgta tactagtgga
    18961 gtacttctcc cattgatgag caaaatgcag aagaagttcg ttttgtaata accaagggcg
    19021 aatcaattac agctattgga gaaaagttga caactgcaaa attgattcgc caccctttgt
    19081 tattcagatt tgctgttcag cagcaacaac ttggccaaaa aattcaggca ggaagttttt
    19141 tactttctcc cgcgcaaaat ctttggcaaa ttgcagaaac gttgacccag ggtactgagg
    19201 atgtatgggt gacgattcca gaggggtatc gagtagaaga aatcgcccaa accttttcta
    19261 atgcaggatt ggtggaattt aatgttgatg actttattgc tcaagctaaa gatcaagagg
    19321 ggtatttatt tccagataca tacttagtgc cgaaattaat taccgctcct gaagttatta
    19381 agttgttaaa tcgaaccttt gatcaaaaag ttcgccaggg attagcagat gaaattgcca
    19441 atagtcagca ttcgctcgag gacgccataa tcatggcatc cttagtccaa cgtgaggctg
    19501 gcaaagatga ggacgagatg aaacaggtag ccggtgtgct ttggaacaga attgccatca
    19561 atcaaggttt gggagtggat gctacattgc agtatattga tggttttgac gagaaaacag
    19621 gtaaatggtg gtcgaacccc gatgtacgta caaaagaaag cacctctcct tataatacct
    19681 acaaatatgc tggattacct ccagcaccca ttgcaaaccc tggagttgca gccatcaggt
    19741 ctgcacttga cccattgaaa actgataatt attactatct gcatgctgag ggaaagattt
    19801 actacgctaa agatttggca gggcactcaa gtaacataga tagatacttg cgttgatgca
    19861 aattgcttgt tataatcctc cccttagcaa aaattagtgg tagctatttt tgaggaacat
    19921 ttgcacaaac aagcctcatt ttatcgagag caacagtaac aatcctaccc acacacagag
    19981 agaatcctat caaaaaggac ttctctgctt ttttgttgtg gggatacaaa cataaggagt
    20041 cggaatgtca gataacactc gccaaaactg gggtaaacac catgtggttg ttcccgaatt
    20101 agaccttgtt ggactccaaa aacaaagcta tcaggaattt ctagaaaatg gtattgccac
    20161 agccttgcgc gaagtgactg gagaaaaggg cattgaagat tacacaggaa aaagttgggc
    20221 attaaagttt ggtggctatc gttttggtga accaaagtac tctgctactg aagcaaaaag
    20281 aaaaggcgta acctacgaca tgcctctcta tgtgcaagcg gagttgttga ataaaaaaac
    20341 tggtaaagtt caagaacaag aggtgttctt gggtgatatc cccaaaatga cgactattgg
    20401 cacattcgtg atcaatggta ttgaaagagc agtagtgacc cagctcgttc gttctcctgg
    20461 tgtatttttt accggcgaaa ctgatgctag taccagaaaa gatctctact ttgccgaact
    20521 tcgaccaaaa cgcggttctt ggttagaaat tactgttggt aagcgagacg ttattgcagt
    20581 caagatcgat cgtcgccgaa aaatgcccgt cactgtgttg ctcagagctc tgggatatgg
    20641 caccgacgat gacataaaag cattatttgc tgacgaaatt ggctttgacg aatttaaatt
    20701 actccaaact actttagaaa aagatactac tcagtctcaa ccagaagcct tattggaact
    20761 ctatgaaaaa atgcgcccag gtgagccagc cgttatcgat acagctcaag agtacgtaca
    20821 tcaattattt tttgatagca gacgatatga tttaggtttg gtcggtcgct acaaattaaa
    20881 ccgcagattg aagttatccg tcgatgaaaa taccacagtg ctaactccag aggacgttgt
    20941 ggcaacagtt aaatacttaa ttggattaca gaatggacga ggtaaaactg atgacataga
    21001 tcacttgag
//
LOCUS       DAOQZP010000013        20876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_2914_length_20876_cov_5.096969, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000013 DAOQZP010000000
VERSION     DAOQZP010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 20876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20876
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2914_length_20876_cov_5.096969"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1093
                     /locus_tag="PKH60_01480"
     CDS             164..1093
                     /locus_tag="PKH60_01480"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNQ16753.1"
                     /translation="MQDNHSTINFSVTEKVLGTNHSQVIIEPLEQGYGHTLGNSLRRA
                     LLSSLPGTAFTTVKFSDVDHQFSTLPGVKEDVLEITLNLKSINLNSLTENGGVARLSV
                     VGPKKVTAADLVCEAGLEVVSPEQHIATVSKGSKLELELIAERGLGYVLAKDRQTNTI
                     GEIMLDALFSPVVKVSYKVESTRVGRRTDFDRLLLDVVTDGSLKPLEAVKKAARILEK
                     QFSQIINPVVQQPDEPQDDLSPEVKEVLRLTVEELDLPTRIANALRKGGFETVGNLST
                     ASRDTIAKVKNLGEKSVDVVQRALEKKGMSLAS"
     gene            1101..1604
                     /gene="rplQ"
                     /locus_tag="PKH60_01485"
     CDS             1101..1604
                     /gene="rplQ"
                     /locus_tag="PKH60_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNQ16754.1"
                     /translation="MRHRIKGNHFNRDTNNRKALLMNLVRSLIEHGSITTTKAKATEV
                     GRISNKMIAKAKNDTIETRRLLHRFFGKRDIVNTLVDTIAPLFTDRNSGFTTEVDLGT
                     RRGDNASLVKLSLLKTPEKNMFLKAPKKTETKAEVKSAKSAKPVSSPVTKKKLEKPKA
                     KVTKKVK"
     gene            1610..2032
                     /gene="rplM"
                     /locus_tag="PKH60_01490"
     CDS             1610..2032
                     /gene="rplM"
                     /locus_tag="PKH60_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNQ16755.1"
                     /translation="MTNITSMLKSAQVKRQWVLVDVENKILGKVATEIATRLIGKQKK
                     EYTPHIDAGDYVVVINAEKVAVSGKKETNKLYQHHTGQPGGLRTKTLGEVRAAHPERL
                     INSAVMNMLPKNKLRAERMKRLKVFAGSEHPYQSQISK"
     gene            2044..2556
                     /gene="rpsI"
                     /locus_tag="PKH60_01495"
     CDS             2044..2556
                     /gene="rpsI"
                     /locus_tag="PKH60_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNQ16756.1"
                     /translation="MALKLNKKKTAPKKIVKKIQRLKTAKSKVGTVELLTPPTGQYFE
                     GIGRRKSAIARVRIYPKVKGDYVVNGKVVGQYFSSLVKPSTRYLLPLEVTGTTGTFAV
                     FARVSGSGISGQLDAVVHGLSRALEKHNPDFHGLLKQAGLLTRDSRQKETRKIGMGGK
                     ARRRRQSPKR"
     gene            complement(2553..3353)
                     /locus_tag="PKH60_01500"
     CDS             complement(2553..3353)
                     /locus_tag="PKH60_01500"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNQ16757.1"
                     /translation="MMLTNLTRYWNFWQKVAQNSLQQAFVNRATNVLFLLGKLVRLFF
                     SLLFLLAIKNNLQWFGSYTTDQLLIFFLTYQIIDVVAQAVFRGVYSFAYAIRNGEFDL
                     TLLKPISPLFQALMGNPDVNDVLFLIPTIGAAFFLINQSSLELTAPHLLVYAALLFNS
                     MLIATALHISILAFGIITMEVDGLTWLYRDFLYLGQLPVDAYKQPIRGLLSFVVPIGI
                     MITVPARQLLGLTNTVGILFTGLVGITTIVISLQLWKFALKKYSSASS"
     gene            complement(3363..4154)
                     /locus_tag="PKH60_01505"
     CDS             complement(3363..4154)
                     /locus_tag="PKH60_01505"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNQ16758.1"
                     /translation="MNKYLALLSISWQNGFAYRASVFLWRFRQLLSSFFSLSIWTILY
                     QNSEASFGYSQSQMISYIFITGIIQNVVITTSLNGLTSTIYSGQLSLLLIKPIGLFKA
                     FAMEETADKAKNLFFALLESCLLFFVFKPQLFSVMPIHFLISIFWIFGGITITFFISL
                     LFGAIGFWSPESWGPRFLFFTFINFLGGKFFPLDILPNLIHQLVWLTPFPFLSYAQSQ
                     LLLNRLSSTEIISGCLGLSLWILLLGLATKYFWRVGLKDYAAQGQ"
     gene            complement(4151..5176)
                     /locus_tag="PKH60_01510"
     CDS             complement(4151..5176)
                     /locus_tag="PKH60_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018034527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ16759.1"
                     /translation="MSEEPIISVNHLSKHFKVYQKQPGLMGSIVSLWSRSYETIHAVE
                     DISFTIHAGEIVGFIGQNGAGKTTTLKMLSGLLFPSSGSVDVLGFNPWDRKPAYQKQF
                     SLVMGQKNQLWWDLPAMETFLLNQAIYEIPNLEFKQTLNQLIELLDVSSVVNVQVRKL
                     SLGQRMKCELIAALLHKPKVLFLDEPTIGLDVVMQKTLRDFIKEYNQQFNATIILTSH
                     YMGDVKELCKRTIIIDKGKKVFDGALQDIIDKYARNKILSLVFSKPITRKELARFGTI
                     TEFSEDGTNDTATIVVPRKESTKIAASIMTKFPVQDVTIEEPPIDAIIREVFKDQNRA
                     SQHHEQS"
     gene            complement(5191..5931)
                     /locus_tag="PKH60_01515"
     CDS             complement(5191..5931)
                     /locus_tag="PKH60_01515"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNQ16760.1"
                     /translation="MKMLEYRSSFIFWVLINILWSLFNILFFSILVGVSGSIGGWNRQ
                     EVFVLLGIFTIIDAFTWSLFYANMTEYAEDIFNGRLSLLLCRPVNTQFYILTKKLNPQ
                     IIFRLMIGAVITLHSLSELNVSLSPLQILLFVFFLMVSLLFLYSFWFMLTTCAFFFER
                     LENLNEIIPAIRRLWQVPKSVYQGAISVSLASIIPFLLITSLPSEILLNRFSYPVLVY
                     YLGITLIIFFLSKVFFNFAVRHYSGMAN"
     gene            complement(6008..6805)
                     /locus_tag="PKH60_01520"
     CDS             complement(6008..6805)
                     /locus_tag="PKH60_01520"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNQ16761.1"
                     /translation="MHRLFRLLFRAGAKTIAFRAETWLWLILEAIPLIILLMIWQGIY
                     AQTNILGGVQFSTVVFYYIFVFLVDMLTAAHFESWRIAEIREGRIDFYLTKPLGYLSE
                     LSIRHVGDKLMSAVLKLPLFVGFIVLIHLLFHPTLPPIQIITLLQSVPLLLGAFILQF
                     FLSTIIVLIGFWLENAEGLDHFRWLSTSLFSGIAIPFAFMPAGLLHLAQLLPFKYLHS
                     VPVLVLLGQQTIRPSDYLYLTVTCFVLGIITLLLWQKARLRYSAYGG"
     gene            complement(6842..7126)
                     /locus_tag="PKH60_01525"
     CDS             complement(6842..7126)
                     /locus_tag="PKH60_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNQ16762.1"
                     /translation="MSKVTKSQLIAIVAKKARLPKRAANDTVEALFDEMIRTLTKGGK
                     VVISGFGTFYVSVVNDKQVVPFGDESKRQTIKGHRVINFRPGKPIKKSVW"
     gene            complement(7237..7584)
                     /locus_tag="PKH60_01530"
     CDS             complement(7237..7584)
                     /locus_tag="PKH60_01530"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNQ16763.1"
                     /translation="MPPLLCAATIFWLSSLSSLPGLQLSIYDFFFKKVAHMIAYATLY
                     FLTRQAIVHSYPEIKSYDWRVWLLPLLICLFYAASDELHQSLIPNRTATIRDVVYDFL
                     GMSMIFLRQNRYI"
     gene            complement(7613..8770)
                     /locus_tag="PKH60_01535"
     CDS             complement(7613..8770)
                     /locus_tag="PKH60_01535"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNQ16764.1"
                     /translation="MNQLWQVTVVVVIVSLFSYTTTAAMASNTPFISEVYPAPNADES
                     EWIELMNPSDEIATISGWLLYDQVSSPSLLLTITTVELAPHEFIVFDLPTAKLNNTGD
                     SVALFNQNGVEVDRFTYNSSEVGKSWQRYAQISEYFLATPTKSTASVPPPPTHAPTPT
                     LSPTASIHPSPTSFIQTDVDFKAIQITEVVACPNTNEREWIKLHNTSDAPISLENWYI
                     LDAQGNQRLIHGVILSKSDLLIDWDGAILNNQGDSISLFTSTDQLSLTLQIPPCIVGA
                     SFFPNQSSSDPPSTHTVALPPTQPSSQVTISATKDTPNGLGANNNSLGEKSFIPPMPS
                     ITIERPQFVSSQTSAPIQIHSTSTIKIPAFLGAIISFSSLLLAYYYFNVFH"
     gene            complement(8828..10042)
                     /locus_tag="PKH60_01540"
     CDS             complement(8828..10042)
                     /locus_tag="PKH60_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16765.1"
                     /translation="MLYRHSLLLALFFFLFSQSSVFACDQYNCEEKKSDTTAYSACLS
                     EKRVCLESKISDIQSQKATLNSTISIISSKITLQELTISQTLSEITKLENDIELLSER
                     ISTLNFSLDQLTAMLLKRVRERYKSSVTSPITLIANSQSFSELITSSRYISQASLQTA
                     VVMRQAELQKQLFDLQKEKKETAQLLLEQKRKLLEQQKRELASQKAGQQKILQDTKNS
                     ESVYQKQLAAVSAEFQAIQAIISGNGSEAKVGDVKAGDTIAFVISGASCNSSGTHLHF
                     TVTENDSVKNPFSYLTSIDHTNCSGSSCGSADGDAFNPSGNLNWPLSGPIRLLQGYAS
                     TWGVQHTWVSRIYTFHNGIDITGSSSTVKAVADGTLYRGSFSGSAGCALRYVKLEHAN
                     SSIVSWYLHIYY"
     gene            complement(10045..10914)
                     /locus_tag="PKH60_01545"
     CDS             complement(10045..10914)
                     /locus_tag="PKH60_01545"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNQ16766.1"
                     /translation="MNSLTTALTTIRRSPYQALIAVLMTSLTFFISFLLSFVVIGAEI
                     VLKHVESQPQIIAFFELGTEQEFIDNMKDKYNKNPQISSVQIVTQEQALDIYRKENSD
                     DPLLIELVTADILPASIEVKTHNLEDLELIKTELDAEDSIEEVIYQQDVIVTLKKWTS
                     GIRTGGIVVASVLAIVSFLSIMIVIALKATNQKNTISIFRLLGATQGFVKLPFIFEGV
                     LYGFMGCLIGWILAFLTIVFSLPQIQAYIGNLFPLSIPLELLAIQLGLGILTSALLGA
                     TAGFVAVSRLMRT"
     gene            complement(10911..11747)
                     /locus_tag="PKH60_01550"
     CDS             complement(10911..11747)
                     /locus_tag="PKH60_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ16767.1"
                     /translation="MSIIQFKNVSKRFLPANDGLMDISFSVDPGEFIFITGPSGAGKT
                     TLLRLLTREYTPTEGSIVFDNEDIVTISKKQLPLHRRKIGVVYQDYRLVPELNIWENV
                     ALPLIINQTPQSTIEQRVTDLLTLVDMTSKALLFPRQISGGEAQRISIARALALAPQV
                     VFADEPSGNLDSETTASIIHLLNKINSLGTTLFLATHDQQVLKMYPKTRIIKLKNGAV
                     EQDTHDKKSESKKDTETKPDNESESEKKEAETVTKDKATSAGFLNKLFKHEKMKKASQ
                     TT"
     gene            complement(11767..12330)
                     /locus_tag="PKH60_01555"
     CDS             complement(11767..12330)
                     /locus_tag="PKH60_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16768.1"
                     /translation="MQEVPSRPIVADEQLVASSNQPSQLNQETINQKGDEMKKLPVKL
                     MLIAGVIAIIGGVATGWGSFGLFAKQSQSGETDATNAPLQQVAGSNVKAGDVFGSGDS
                     STFTDFAEGYLESGGIDGEGSHKLLRSGGPSQTVYLTSSVTDLDKLVGMEVKIWGETF
                     KGQKAGWLMDVGKIEVVNPKAEPPQQQ"
     gene            complement(12360..13457)
                     /gene="prfB"
                     /locus_tag="PKH60_01560"
     CDS             complement(12360..13457)
                     /gene="prfB"
                     /locus_tag="PKH60_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNQ16769.1"
                     /translation="MQNHQTIEDTITKAKAILADQDLSDLESKKIVLESQTLEPAFWQ
                     KTDAQLIIQELAEYSNQLKQFQELNQVLQDTQDLEKALMDQSLLDSDRQSFELEFEKQ
                     KQLLVKLVTALEFTQYLSGEYDRLGCVFSIHSGQGGTEAMDWADMLRRMYTRYFERKQ
                     WKFQLISEVRGEEAGIKQAEYEIVAPYTYGFLKHERGTHRLVRQSPFNADNLRQTSFA
                     LVEILPLIPASDKTIEVKEDELEWKFSRSGGAGGQNVNKVNTAVELRHVPTSLVVRCQ
                     EERSQVQNKEKALQKLKSLLAQRKEDERQQKLQTEKGSHANASWGTQIRNYVLHPYKL
                     VKDTRTGVETSQAEQVLDGDLDDFIDAAVRL"
     gene            complement(13479..15128)
                     /locus_tag="PKH60_01565"
     CDS             complement(13479..15128)
                     /locus_tag="PKH60_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HNQ16770.1"
                     /translation="MKIRPASKKHIALGLFLWSLIILALFLTVLLPPLVLTRMGVSSF
                     LSGNFDVAAKRWQQARLIVDPLSSLTQPLVTDGVLWQASLSLAEQLANDLHSFNQQKQ
                     QSIYDLPTDVLVSHIPNYLLMFDSIEANFESSIFVKQVFKQKKVLEKISLIKEWLGAV
                     SPVLSNKSKWIILLQNDNELRATGGFMGSYAVITINQGKITELTIEDIYDADGQFSGY
                     FPAPVGVKEYLSSGNGLRLPDANWHPDFPTSAQQVLGYFALGNRSEISGVIAVTNSVI
                     GEVLKITGPLQLPDYHTNITPDTLDAVLQNRPQAFFPGSIQKKHMLSQAKNQLVAAVT
                     KFTLNDWLSLLAILNNEIHTKNILFFSVNDSLNSLFDTYSMSGKLATQSNNQDLLALI
                     ESNVGINKSNQWQHRSVTVDVSKPTQAIITLQYSNQSPESITFEQSRYVNYLRLITDV
                     NWILESVQEDNNNPATEWQQTPLLTKTGNQFSETGGLLEVAPRSTKVVTITFKRESSQ
                     AASLLLVKQPGLPEVHYTIIKPDGSSEQLVVESNQEFFITK"
     gene            complement(15133..15615)
                     /locus_tag="PKH60_01570"
     CDS             complement(15133..15615)
                     /locus_tag="PKH60_01570"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009015312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNQ16771.1"
                     /translation="MKQLSDFPPNKATKAVLSTTKGDITISLFRDEAPLTTLNFITLA
                     SSSFYDGIVFHRIIPGFMAQVGDPISKDPAQKSRWGTGGPGYSIADEFGPGLKHDKAG
                     VVSMANSGPNTGGSQFFITHEETPWLDGKHAIFGQVTEGMEVVMQLEVGDSITKITLQ
                     "
     gene            15905..16633
                     /locus_tag="PKH60_01575"
     CDS             15905..16633
                     /locus_tag="PKH60_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNQ16772.1"
                     /translation="MKDTQILILHGWTSSDQTKDKWQPLIKLLEEKKYSVRLLLLPGL
                     TQPLSESWDLEQYVKWLDAQVSVNKQVIIIGHSFGGQLAIKYAAHHPEHLKCLLLIDS
                     SGVPDNRWQKKLKRLVFLTLAKVGRNLPQKKFLKKLLYRLAGERDYYQATEKQKATMR
                     LAIRTDIREEASKIKIPTLLLWGENDQTTPIFMGKTLAMLMQNSQLQIIPNARHSPQY
                     THPMQVTSIFHNWLQQLNSNASKV"
     gene            16636..18135
                     /locus_tag="PKH60_01580"
     CDS             16636..18135
                     /locus_tag="PKH60_01580"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNQ16773.1"
                     /translation="MILFKITLTIVGLIRLLRWLSIIQEKEYRWDRLKIFIFSSKGLR
                     ELVRVIPRPSDFTRSGLKRPKRTGRVLVIAVIAISYIYNLIQISDTWVGQLDYQLGAD
                     LMTSLFLLSIYILIPTFVLLAIIPTVVISSFQTNLTLLRAKRKLNQQKPMVIGITGSF
                     GKTATKQLLAHILNEVEPVFATPKSFNTRYSVARSILKGYKSEKIAILEYGAYAKGEI
                     AKLASWFQPTIAVITGLTSQHLGLFGTQQAIIEAKSELITSLPRQGAVVYNALDEGVQ
                     QIVTLGVHNWQIKHHTNQEPILIGCNPKMSKQTFGYVLEKSGHLAILAGKRKIITDLI
                     GMQYAQTVELAVTIGARLKLSEAAILHAISTFSPGDNFIQIRKTKSGGTIIDDGGTAN
                     PAGFKAALELLQQSENKNKWLITPGIVDLGLESKSVHQKLADFSASFVQKVLYVDLNG
                     RAEFEAKFGTNCETDRELIIKRLKQMDSDVVLLIEGRMPGWVQPYIKAL"
     gene            18132..19337
                     /locus_tag="PKH60_01585"
     CDS             18132..19337
                     /locus_tag="PKH60_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNQ16774.1"
                     /translation="MKRFNIFNSLGSNYSWRDARIALRGFFSPEKSSLEQLITLLTQA
                     FPGYSVVPTYKGRDALELSLRSLGVGVGDVVLTQAFSCYAVEEAIIRCGAKPVYYDID
                     RAMLVPTTATLDQALHRHEVENSNGVKVAAIIAQFTLGNISSSNVIVNWSKKHQIAYV
                     ADLAQAIGAKQNETLLGNGADAIVLSTGRDKVWDGVSGGFALTRDPIHSSLLPRDAVG
                     LIQTIRDCVYPILTILIRKLYPIYLGKLLHFLVTKMGVFYSPIETIYRRTTQMPQGLA
                     TFLLHRWPTIESDLPHRKVIAKVYYKLLSEHSLIDEKGIEDGTNLRFPVYVENPREII
                     DKLQEFGIYIADRWYRKPVDSGSFPYKSSYEPGSCPNAEIIANHCINLPTHQLINPKI
                     AKQIAELVL"
     gene            19334..20347
                     /locus_tag="PKH60_01590"
     CDS             19334..20347
                     /locus_tag="PKH60_01590"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNQ16775.1"
                     /translation="MKLTQCNSLGEWNLCIRHFAEANFLQSWQWGEFQQSLGKAVFRF
                     VVLSGSQPIALVQVVREPAKRGAYFAIAGGPLLDWNNHEVVTFLFAEIKKLALQHNCV
                     FIRFRPQVEQRLELRQLLHSIGAVTAPMHLTADLTIEIDLTKSDEELKAEMRKQHRQA
                     LKKATELGVHVQLSKNIKDLKQFYQEQLVVAQRQGFVPFSERFLTKQFEAFLADDQIV
                     LFHAYYEKTLLASAFVIFYNGEAVYHYGISTSENREYPGSYACQWAIIQEAKKRGCSR
                     YNLWGVSPKDAIHHRFYGVGLFKRGFGGKEVAYVPAQDIPLNWKYWLTWIFETLRRKM
                     RKL"
     gene            complement(20352..>20876)
                     /locus_tag="PKH60_01595"
     CDS             complement(20352..>20876)
                     /locus_tag="PKH60_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16776.1"
                     /translation="YYAGSKSYDTDIERAIERLLEELPREKLSFSLTTTTLFEEQATT
                     IQSEWQNLGSLAVERCNQSSQVQDKSLCANLGIEVQLKIANFPDTSTFDTILIGQEAP
                     PDPDQYELWHSNEPTNFSGYKNTRIDNLLEKGRQTNSRDERIQLYQEFQQFLTEDAAA
                     IFLEHLTSYRIERL"
BASE COUNT         6406 a         3851 c         4430 g         6189 t
ORIGIN      
        1 caacagtcga agctctattg aaagatggtg tttcgagagt tgagtggctt gaaaggaaag
       61 ctcacattgg caaggtggtg agagccccac tgcgcaatga tattcaagaa aaaattgaag
      121 aacaattagt aatcgaatat tatagtcgtt aactggagta tgtatgcaag ataatcattc
      181 aacaattaat ttttctgtta cagagaaggt tttaggaact aaccattctc aggtaattat
      241 tgaaccacta gagcagggct acggtcacac attgggcaat tctttgcgtc gcgcattact
      301 gagttcgtta ccaggaaccg cattcacgac agttaaattt tctgatgttg accatcagtt
      361 ttcaactctt ccgggtgtta aagaagatgt gttggaaatt actttgaatc tgaaatcgat
      421 taatcttaat tcgttaaccg aaaacggtgg cgtagcaaga ctttcagttg ttggtcctaa
      481 aaaagtaaca gccgcagatt tagtttgtga agctggtctt gaagttgtta gcccagaaca
      541 acatattgct acggtcagca aaggttcgaa gcttgaatta gaactaatag cagagcgggg
      601 attgggttat gttttggcaa aagatcgtca aacgaacact attggtgaaa ttatgcttga
      661 tgcattattt tcaccagttg ttaaagtttc gtataaggtt gagtccactc gtgtaggacg
      721 acgtacggat tttgatcggt tattgctaga tgttgtgacc gatggctcgc ttaaaccact
      781 cgaagccgta aagaaagccg ctcgaatttt agagaaacaa ttttcgcaaa ttattaaccc
      841 agtagttcaa caacctgacg aacctcaaga tgatttatct ccagaagtta aagaagtgtt
      901 gagactaact gttgaggaac tagatttacc caccagaatt gcgaatgctc tccgaaaagg
      961 tggatttgaa acagtgggca acctcagcac tgcatcaaga gatactattg cgaaggttaa
     1021 gaatcttgga gaaaagagtg tcgatgtagt tcagagagca cttgaaaaga agggtatgag
     1081 cttagcctca taatcattgc atgagacatc gaataaaagg caatcatttt aatcgtgaca
     1141 cgaacaaccg caaggctttg ttaatgaatc tagtgaggtc cttgatagaa catggttcca
     1201 ttaccaccac taaggctaaa gcgactgagg ttggaagaat tagcaataaa atgattgcta
     1261 aggctaagaa cgataccatc gaaacaagaa gactgttgca tcgattcttt ggcaaaagag
     1321 atattgtgaa tacattggta gatacaattg ccccgctttt taccgacaga aattccggtt
     1381 ttactaccga agttgatttg ggtactcgtc gtggcgacaa cgcttcattg gtcaaactct
     1441 cacttttaaa aactccagag aagaatatgt tcttaaaagc gccaaagaag acggaaacaa
     1501 aagctgaggt gaaaagtgca aaatctgcaa aaccagtttc aagtccagtt accaagaaaa
     1561 agctggaaaa gcctaaggca aaagtaacta aaaaggttaa ataattatca tgacaaatat
     1621 cacctcaatg cttaaatcag cacaagtgaa acgccaatgg gtattggttg atgtagaaaa
     1681 taaaatttta ggtaaagttg caactgaaat tgctactcgc ttgattggta agcaaaaaaa
     1741 agagtacaca ccacatattg atgctggaga ttatgttgta gtcattaatg ctgaaaaagt
     1801 agccgtctct ggtaaaaaag aaacaaataa attgtaccaa catcacactg gtcagccagg
     1861 tggtttacgt acaaagacat tgggagaagt tcgtgcggct catccagaaa gattaattaa
     1921 ttcggcagtc atgaatatgt tgccaaagaa caaattgcgc gcagagagaa tgaaacgatt
     1981 aaaagtattt gctggctcgg agcatccata tcagagtcaa atttcaaagt aggaacaaga
     2041 gttatggcac ttaagttaaa caagaaaaaa actgcaccga aaaagattgt taaaaagatt
     2101 cagcggctaa agactgctaa atcaaaagtt ggcactgtgg agctattaac gccaccaacg
     2161 ggacaatact ttgagggtat tggaagacgt aaatctgcaa ttgctcgtgt gcgaatatat
     2221 cccaaggtta agggtgatta tgttgtgaat ggcaaggtgg tggggcagta tttttcctca
     2281 ttggtgaaac catccactag atatctcctt ccgttagaag ttactggtac aactggtaca
     2341 tttgccgtct ttgcgcgagt aagtggatca ggtatctcgg gtcagttgga cgctgttgtt
     2401 catgggttga gcagagcatt agaaaaacac aaccctgatt ttcacggttt actaaaacaa
     2461 gcaggactac tgacaagaga ttctagacaa aaagaaacca gaaaaattgg catgggtgga
     2521 aaagctcgtc gaagacgaca gtctccgaag cgctagctac tcgcactgct gtattttttt
     2581 aatgcaaatt tccacaactg tagactaata actattgtcg taatacctac taaacccgta
     2641 aataaaattc ccacagtgtt tgttaacccc agtaattgac gtgctggtac ggtgatcata
     2701 attccaatag gtacgacaaa tgataataat ccccgtattg gttgtttata ggcatctacg
     2761 gggagttgac ctagatacag aaaatcacga tagagccaag tgagtccatc cacctccatt
     2821 gtgataatgc caaaggctaa gatgctaatg tggagcgctg tggcaataag catactgttg
     2881 aagagtagcg ccgcatatac aagtagatgc ggcgccgtca attccaatga agattgatta
     2941 atcaagaaaa aagccgcacc aatagtggga attagaaata gcacatcatt aacatcagga
     3001 ttacccatca atgcctgaaa taagggacta attggtttaa gtaaggtgag atcaaactct
     3061 ccatttctaa ttgcatacgc gaaactatag acgcccctaa aaacagcttg agcaactaca
     3121 tcaataattt gataggtaag aaaaaaaata agtaattgat cggtggtgta actgccaaac
     3181 cattgtagat tgtttttaat tgcgagtaga aaaagcaatg agaaaaataa gcgtactaac
     3241 ttgccaagta aaaataaaac attagttgct cgatttacaa aagcttgctg taaactattt
     3301 tgagcaactt tttgccaaaa gttccagtag cgggttagat tggttagcat catatatctt
     3361 tgttattgtc cttgagcggc gtagtctttc agtccaactc tccaaaaata tttagtcgcc
     3421 aatccgagta gcagtatcca aagagaaaga cccaagcatc cagatataat ttcagttgat
     3481 gaaagtcggt taagtaataa ttgtgattgc gcataggaga gaaagggaaa gggagttaac
     3541 caaaccaatt ggtgaatgag atttggcaaa atatcgagag gaaaaaattt tcctccgaga
     3601 aagttaataa atgtaaaaaa taaaaaccgt ggaccccaag attctggact ccaaaaacca
     3661 atagcaccaa ataataacga gataaaaaat gtaattgtta tgcctccaaa aatccaaaaa
     3721 attgatatta aaaaatgaat tggcatcact gaaaatagtt gcggcttaaa cacgaaaaaa
     3781 agcaggcagg attctaataa agcaaaaaat aaatttttag ctttatccgc agtttcttcc
     3841 atggcaaagg ctttaaacaa tccaattggt ttgattagca ataaactgag ttggcctgaa
     3901 tagatggtag aagtcagtcc atttaaagat gtggtgataa ctacgttttg aataatgcca
     3961 gtaatgaaaa tatatgaaat catttgtgat tgagagtatc caaatgaagc ttctgaattc
     4021 tggtagagga ttgtccaaat agacaaagag aaaaatgatg aaagcagttg tctaaatctc
     4081 cataagaata cacttgctcg gtatgcgaaa ccgttttgcc aagagataga aagtagcgcg
     4141 aggtatttat tcatgattgc tcatgatgtt gagaagcacg attttgatcc ttaaacacct
     4201 ctcgaataat agcatcaatc ggaggttctt caatggtaac atcttgaaca ggaaatttag
     4261 tcataatact ggcggctatt tttgtcgatt ctttccttgg tacaacgatt gtggcggtat
     4321 cgttggtgcc atcttcagaa aactcggtaa tcgttccaaa tctagcaagt tctttgcggg
     4381 tgattggttt tgaaaacaca agtgaaagaa tcttatttct ggcatattta tcgataatat
     4441 cttgtaaggc cccgtcaaaa acctttttac ctttatctat aataatcgta cgcttgcaca
     4501 attccttaac atcccccatg taatgactag ttagaatgat tgtggcatta aattgctggt
     4561 tatattcttt aataaaatct ctcagtgtct tttgcataac tacatccaga ccaatggtgg
     4621 gttcatctaa gaaaagcacc tttggtttgt gcaagagcgc ggcgatcaat tcacatttca
     4681 ttcgctgacc gagtgaaagt tttcttactt ggacattaac cacggaagaa acatcgagta
     4741 actctattaa ttggttgagt gtttgtttaa attctaaatt tggaatttca tagattgcct
     4801 gatttaataa aaacgtttcc attgctggca aatcccacca caattgattc ttttgaccca
     4861 ttactaatga aaattgtttt tgatacgctg gctttctatc ccagggatta aacccaagaa
     4921 cgtcaacgga gccagaagaa ggaaaaagca gtcctgataa cattttgagc gtagtggttt
     4981 ttccagcacc attttgtcca ataaaaccaa caatttctcc agcatgtatt gtgaatgaga
     5041 tatcctcaac agcatgtatg gtttcatatg atcggctcca gagtgagacg attgagccca
     5101 tgagaccagg ttgtttttga tacaccttga agtgtttgct gaggtgattg acggagataa
     5161 ttggctcttc agacatggtg ttatcttacc ttagtttgcc attccactgt agtggcgaac
     5221 agcaaaattg aaaaatacct tagaaaggaa aaagataatc agagttattc ccaagtagta
     5281 gactaggact ggataagaaa aacgattgag gagaatttcg cttggcagtg aagtaatgag
     5341 taaaaaagga ataatactgg caagtgacac gcttatcgct ccctgataaa cacttttggg
     5401 aacttgccac aacctgcgta tggcgggaat aatttcgttg aggttttcta agcgttcgaa
     5461 gaaaaaagcg caggttgtta acataaacca aaaactatat aaaaataaca agcttaccat
     5521 cagaaaaaaa acaaacaaca gtatttgcaa tggtgaaagt gaaacattaa gttcggaaag
     5581 actatgaagc gtaatgacgg ctccaatcat aagtcgaaaa ataatttgag gattgagttt
     5641 ttttgtcagt atatagaatt gagtgttgac cggcctgcag aggaggagtg aaagccgacc
     5701 attaaaaata tcttccgcgt attcggtcat attagcgtaa aacagtgacc acgtaaacgc
     5761 atcaatgata gtaaatattc caagcaatac gaaaacctcc tgtcgattcc atcctccaat
     5821 tgagccggaa acaccaacca gtattgaaaa gaacagtatg ttaaagagac tccacaaaat
     5881 gttaatcaaa acccaaaaaa tgaaactgct acgatactca agcattttca taatgtctat
     5941 gtaacgaaat ctaatgaata atcgcaggtg acgctggatc actgaagaga atggctggga
     6001 atgtgtttta tccaccatat gcggagtatc gcagtctggc tttttgccat agtaaaaggg
     6061 ttattattcc aagcacaaaa caggtgactg ttaagtagag gtaatcgcta ggcctaatag
     6121 tttgctgtcc aagtagcacg agcaccggca cactatgaag gtatttgaaa ggtaagagtt
     6181 gggcaagatg aagtaatccc gctggcatga acgcaaaggg aattgcaatg ccactaaaca
     6241 acgatgtgct gagccaacga aagtgatcca atccttcggc attttctaac cagaatccaa
     6301 tgagtacaat aattgtggat aagaaaaatt gtaaaatgaa ggctcccaga agaagtggga
     6361 cagattgtaa caaagtaatt atttgtatag gtggtaacgt tgggtgaaat agcaagtgaa
     6421 tcagtacgat aaatccaaca aataatggta gctttaaaac tgcgctcatg agcttatctc
     6481 caacatgacg aatacttagc tcagacaagt atcccagcgg tttggttaga taaaagtcaa
     6541 ttcgaccctc tctgatttca gcaattctcc aactttcaaa gtgagctgca gttagcatgt
     6601 ctaccaaaaa aacaaagata tagtaaaaca caactgtact aaattgaact ccacccaaga
     6661 tatttgtttg tgcataaata ccttgccaaa tcatgagtaa aattataaga ggaattgctt
     6721 ctaaaattaa ccagagccac gtttctgccc tgaaagcaat tgtttttgct ccagcacgaa
     6781 acaataaccg aaaaagacgg tgcatggtca gaatcctacc atagttttga agggtaatgc
     6841 tttaccaaac gctttttttg attggtttgc ctggtctgaa attaatgact cgatggcctt
     6901 taatggtttg gcgttttgat tcgtcgccaa atggaacaac ttgcttatca ttaacgacac
     6961 tcacataaaa agtgccaaag ccagaaataa caacctttcc acctttggta agtgtacgaa
     7021 tcatttcatc aaatagggct tcgacggtgt cattagcagc acgcttgggc aagcgagctt
     7081 ttttggccac gattgcgatt aattgtgatt tagttacttt gctcataaga cgctcctttt
     7141 tctacttctt tgtacagcaa cttgatacgg caaagtgtaa atgattgaag catcaagtca
     7201 agacccactt tgttgataga ttgtagtagt atgaagctaa atgtagcgat tttgtcttaa
     7261 aaaaatcatg ctcataccaa ggaaatcata cacaacatct cttatggtgg ctgttcgatt
     7321 ggggataagc gattgatgca actcatcgct agcggcgtaa aacaaacaga taagaagtgg
     7381 taaaagccat accctccagt cataagattt gatttctgga tatgagtgca ctattgcctg
     7441 ccttgtaaga aaatagagtg tggcataggc aatcatatga gcgacttttt taaagaaaaa
     7501 atcatatatg gaaagttgta ggcctggtaa agaggaaagt gaagaaagcc aaaaaatagt
     7561 cgctgcgcaa agcagtggcg gcatccaatg aattatgtgt cgaattactg gcttagtgga
     7621 aaacattgaa ataataatat gctaataata aactgctaaa actaattata gcacccagga
     7681 aagcaggtat tttgattgtt gatgttgagt gtatttgaat tggtgccgaa gtttgtgaag
     7741 atacgaactg aggtctttct atcgtaattg aaggcatggg tggaataaat gatttttcac
     7801 ccaaactgtt attgtttgcg cccaaaccat ttggagtatc cttggtggct gaaattgtaa
     7861 cttgtgatga gggttgagtg ggaggcaaag ccactgtgtg agtggagggg ggatcagaag
     7921 agctttggtt aggaaaaaaa gaagctccaa cgatgcaggg ggggatttgt agagttaggg
     7981 aaagttggtc ggtgctagta aagagagaga tggaatctcc ttgattgttg agtatcgccc
     8041 catcccaatc gattaacaaa tctgatttgg acaaaataac tccatgtatt aatcgctggt
     8101 taccttgggc gtctaaaata taccaattct ccaaagaaat aggggcatcg cttgtattat
     8161 gtaactttat ccactcgcgc tcattggtat tggggcaagc cacaacttca gttatttgga
     8221 ttgctttgaa atccacatcg gtttgtatga aagaagtggg gctcgggtga atcgaggctg
     8281 tgggagatag cgtaggcgtg ggagcgtggg tgggtggtgg gggcaccgag gcagtagatt
     8341 ttgtgggcgt agctaggaaa tactcggaga tttgagcata tctttgccaa cttttaccca
     8401 cctcactgga gttgtaggta aatcgatcga cttcaactcc attttgattg aatagtgcca
     8461 cgctatcgcc agtattgttc aatttggctg ttgggagatc aaacacaata aactcatgcg
     8521 gagctagttc tacagttgta atagtgagta gtagcgatgg actactaact tggtcgtaga
     8581 gtaaccaccc agaaattgtt gcgatctcat cggatggatt catcagttca atccactcac
     8641 tttcatcagc attgggagcg ggatagactt cgctaataaa tggcgtatta gacgccattg
     8701 ctgccgttgt ggtgtaacta aaaagtgaca caatcaccac aaccactgtg acttgccata
     8761 attgattcat taatcactaa cggtacttca taaagcttac ggaaagcgtg ataagttcgg
     8821 aaccagtcta ataataaata tgaagatacc aactcactat tgatgaatta gcatgttcta
     8881 acttgacata ccgaagtgcg caaccagcac ttccagagaa actacctcgg taaagcgtgc
     8941 catccgccac tgccttcaca gtcgatgaac taccagtaat atcaatgcca ttatgaaagg
     9001 tatatattcg gctgacccag gtgtgttgaa cgccccaagt tgaagcgtat ccttgcaaca
     9061 agcgaatggg gccggataat ggccaattaa gattaccaga ggggttaaaa gcgtctccat
     9121 ctgcgctgcc acaactagag ccagagcaat tcgtatggtc aatagatgtc aggtaagaaa
     9181 aaggattttt aacgctgtca ttttcggtga ctgtaaagtg caaatgcgta ccgctggaat
     9241 tacatgatgc tccagaaatt acgaaggcaa tggtatcgcc agctttcaca tcaccaacct
     9301 ttgcttccga gccgttgcca gagataattg cttggattgc ttgaaattca gcagaaactg
     9361 ctgcgagttg tttttgatac acgctctcac tattttttgt atcctgaagt atcttttgtt
     9421 gtcctgcttt ctgactagct agctcacgtt tttgttgctc taataatttt cgtttttgtt
     9481 ccagtaaaag ctgagccgtc tccttttttt ctttttgaag atcaaataat tgtttctgta
     9541 gttctgcttg ccgcatcact acagcggttt gcaagctggc ttgactaata tatcttgaac
     9601 ttgttatgag ctcagagaaa gattggctat ttgcaatcaa tgttatgggt gaagtgactg
     9661 atgatttata tctttctcta actcttttta gtagcattgc agtaagttgg tcaagagaaa
     9721 aattgagtgt actaatcctt tcactcaaca actctatgtc gttttctaat ttagtgattt
     9781 ctgacaatgt ttgagaaatt gtaagttctt gaagcgtaat tttgctacta ataatcgaaa
     9841 tagtggaatt taatgtagct ttttgtgact gaatgtctga aatctttgat tctaaacaaa
     9901 cccttttttc tgaaagacat gctgaatatg cagtagtgtc tgatttcttt tcttcacagt
     9961 tgtattgatc gcaagcaaat acgctacttt gtgaaaacaa gaaaaaaaag agggcgagca
    10021 gtaatgaatg gcgatatagc atgatcaggt gcgcattaag cggcttacag ccacaaaacc
    10081 ggcggtagca ccgagtaagg cactcgtgag tatacctaag cctaattgaa ttgccaagag
    10141 ctctagtgga attgataggg gaaatagatt tccaatatac gcttgaattt gtgggagtga
    10201 aaatacaatg gttagaaagg ctaaaatcca gccaattaga caacccataa agccgtataa
    10261 gacaccctca aaaataaaag gtaattttac aaacccctga gtagctccca gtaaacggaa
    10321 aatagaaata gtattctttt gattagtagc ttttaaggcg ataactatca taattgatag
    10381 aaaggaaact attgcgagga ccgatgcaac aactatgcca ccagtgcgga ttcccgatgt
    10441 ccactttttc agtgttacta ttacatcttg ttggtagatg acttcttcaa ttgaatcttc
    10501 cgcatccaat tctgttttaa tgagttctaa atcttctaaa ttatgggttt ttacctcaat
    10561 gcttgcggga agaatgtcgg cggttaccag ttcaatcaag agggggtcat cgctattttc
    10621 tttgcgataa atgtccaaag cttgctcttg ggtcacaatc tgtaccgagc tgatttgtgg
    10681 atttttatta tatttatctt tcatattatc gataaattct tgctcagtac caagctcaaa
    10741 aaacgcgatt atttgaggtt ggctctcaac atgcttgagt acaatctcag cccctatcac
    10801 aacaaaagaa agtaagaatg aaataaaaaa cgttagtgat gtcattagta cagcaattaa
    10861 agcttgatat ggcgaacgac gtatggtggt tagtgcagtt gtgagtgagt tcatgttgtc
    10921 tgagaagcct ttttcatctt ttcatgtttg aacaacttat tcaagaagcc ggcactcgtc
    10981 gctttatctt ttgtcacagt ttctgcttct tttttttcag actcagattc gttatctggc
    11041 tttgtttcag tatctttttt tgattctgat tttttatcgt gtgtatcttg ttctactgca
    11101 ccatttttaa gtttaataat tcttgttttt ggatacattt ttaacacttg ttgatcgtgg
    11161 gtggcaagaa ataaagtagt gcccaagcta ttaatcttgt ttagtaaatg aatgattgag
    11221 gcagttgttt ccgaatcaag attgcccgag ggttcatcag caaacaccac ctgcggtgct
    11281 aaagccagcg ctctggctat actaatgcgt tgggcttctc caccagaaat ttggcgagga
    11341 aagagtaatg cttttgaagt catatcaacc aatgttagga ggtcagttac tctttgctca
    11401 atggtggact gtggggtttg attaataatc agcggtaagg caacattttc ccaaatattt
    11461 aattcgggta cgagacgata atcttgataa acaacgccga tttttctgcg atgaagcgga
    11521 agttgtttct tacttatggt gacaatatct tcattgtcaa aaacaattga accttccgtt
    11581 ggagtgtatt ctctggtgag tagtcgcagt agtgtggtct ttccagctcc acttggaccc
    11641 gttataaaaa taaattctcc agggtctaca gaaaacgaga tatccatcaa gccatcattc
    11701 gctggcagga agcgcttgct gacatttttg aattgtatga ttgacacgct tatatgaata
    11761 ccattactac tgttgctgcg gaggttcggc tttcggattt actacctcaa ttttgccaac
    11821 gtccattaac caaccagcct tttgaccttt aaatgtttct ccccaaattt taacttccat
    11881 tccaaccagt ttatcaagat ctgtaaccga tgaagttaga tacacagttt gagaaggacc
    11941 tccggagcgg aggagtttgt gtgaaccttc tccatcaatt cctccagatt ctaggtaccc
    12001 ttcggcaaaa tcagtaaaag ttgagctatc tccactgcca aatacatctc ctgccttgac
    12061 attcgatccg gctacttgtt gtaagggagc attggtagca tctgtttcac cactttgtga
    12121 ttgcttggca aataagccaa aactacccca accagtggca acaccaccaa taattgctat
    12181 cacaccagca atcagcatta attttactgg tagttttttc atttcatcac ctttctgatt
    12241 gattgtttct tgatttaatt gcgaaggttg gtttgaggaa gccaccaact gttcatcagc
    12301 tactattggt cttgagggta cttcttgcat tcgatcattg tagcaaattt acagcaaact
    12361 tacagcctca ctgctgcgtc gatgaaatca tctaaatcgc cgtcgagcac ttgctctgct
    12421 tgagatgttt ccacaccagt gcgagtatct ttgactagtt tatagggatg taaaacatag
    12481 tttctaattt gggttcccca actagcattt gcgtgagaac ctttttctgt ttgaagtttt
    12541 tgttgtctct cgtcctcttt tctttgagcc aataaacttt ttaatttttg caaggctttt
    12601 tctttatttt gaacttgact tctctcttct tgacatcgaa ccactagtga ggtgggcacg
    12661 tggcgcagtt ctaccgctgt attgacttta ttgacgtttt ggcctcctgc gccacccgaa
    12721 cgagagaact tccattccaa ctcatcttct ttgacttcta tagttttgtc tgatgctggt
    12781 ataaggggta aaatttcaac cagtgcgaaa gaagtttggc gtaaattatc tgcattgaaa
    12841 gggctttgtc taactaatcg atgcgttcct ctctcgtgtt ttaaaaagcc ataggtatac
    12901 ggtgctacta tttcgtattc tgcttgcttg atgcccgctt cttctcctcg tacctctgat
    12961 atgagttgaa atttccactg tttacgttca aaataacgcg tatacatccg tctgagcatg
    13021 tctgcccagt ccatggcttc agtaccaccc tgtcctgagt gaatagagaa tacacagcca
    13081 agtcgatcat attctccaga gagatattga gtaaattcca atgcagttac gagcttgact
    13141 aataactgtt tttgcttctc aaactcaagt tcaaaggatt gtctatcact atctaataaa
    13201 ctttgatcca tcaatgcttt ttccagatct tgagtatctt ggagaacttg attgagttct
    13261 tggaattgtt ttaattgatt agaatattct gccaactctt gaataatcag ttgggcgtca
    13321 gttttttgcc aaaaggcagg ctctagcgtt tgtgattcca aaacaatttt ttttgattct
    13381 aggtcactta aatcctgatc tgccaagatt gccttagcct tagtgattgt atcctctatg
    13441 gtttgatgat tttgcattat ttgcattcta cactaggctt acttagtgat aaaaaactct
    13501 tgattactct ctacaacaag ttgttcactg gagccatcgg gtttgatgat ggtgtaatga
    13561 acctctggga gtccgggttg ttttactagt aataggctgg ccgcttgtga agattctcgc
    13621 ttaaatgtga tagtaacaac ttttgttgat ctgggagcga cctcaagcaa tccacctgtt
    13681 tcagaaaatt gatttccagt cttggtcagc agaggtgttt gttgccattc agtggcgggg
    13741 ttgttattgt cttcctgcac ggattctaga atccaattta catctgtgat caagcgcaga
    13801 taatttacat atcttgattg ttcaaatgtg atgctttcgg gtgattgatt gctatattga
    13861 agggtgatga ttgcctgagt tggttttgac acgtcaactg tgacagaacg atgttgccac
    13921 tgattgctct tattgatacc cacattggac tcaatcaacg ctagtaaatc ttgattgttg
    13981 gattgtgtag ccaattttcc actcatggag tatgtatcga agagtgaatt gagtgaatca
    14041 tttactgaga aaaataaaat attttttgta tgaatctcat tgttaagaat tgctaacagt
    14101 gacaaccaat cattcaaggt aaacttggta actgctgcta ccaattggtt cttagcttga
    14161 gaaagcatgt gtttcttttg aatactgcca ggaaaaaaag cctgcggccg attttggagc
    14221 actgcatcaa gagtatcggg ggtgatgttg gtatgatagt ctggtagctg gagtggtcca
    14281 gtgattttca gcacttctcc tatgactgaa ttggtgactg cgatcactcc actgatttcc
    14341 gagcggtttc ccagcgcaaa ataaccgagc acttgctggg cgctggtggg aaaatctgga
    14401 tgccagttgg catccggcaa gcggagacca ttgccactac ttaaatattc ttttactcct
    14461 actggtgcgg ggaaatagcc agaaaattgg ccatcggcgt cgtagatatc ttcaatggtc
    14521 agttctgtta ttttaccttg attaatagta ataacagcat atgatcccat aaagccacca
    14581 gtggctcgta gttcattgtc attttgcagt aatatgatcc atttagattt attgctaaga
    14641 accggtgata ctgccccgag ccattctttg atgagtgata ttttttctaa aacttttttt
    14701 tgtttaaaga cttgtttaac gaatattgat gattcgaaat tagcttcaat actatcaaac
    14761 atcaacagat agtttgggat atgagacacg aggacatcgg ttggaagatc gtaaatactt
    14821 tgttgttttt gttgattgaa cgaatggaga tcatttgcga gctgttcagc caaggataaa
    14881 ctggcttgcc agagaactcc atcggtcacg agtggttggg taagagagga taggggatct
    14941 acgattaaac gagcttgttg ccatcttttc gcagcaacat caaaatttcc cgatagaaaa
    15001 gaggagactc ccattctagt tagaactagc gggggtaaaa gcaccgttaa aaatagcgcc
    15061 aagataatta acgaccaaag gaataatccc aaagcaatgt gcttttttga tgcagggcgt
    15121 attttcacat tactattgta acgtgatttt ggtaatcgag tcaccaactt caagctgcat
    15181 gacaacctcc atgccttctg ttacttgacc aaaaattgca tgttttccat ctagccaagg
    15241 ggtttcttca tgcgtgataa aaaattgact gcctccagtg tttggtccag agtttgccat
    15301 agagactacc ccagccttat catgttttaa gccagggcca aattcatcag ctatagagta
    15361 gcctggtcca ccagtgcccc aacgactttt ttgagcggga tcttttgaga tgggatctcc
    15421 aacttgcgcc ataaacccag ggataattct gtgaaagacg atcccatcgt agaaacttga
    15481 actagctaac gtaatgaaat taagcgtagt gaggggtgcc tcatctctaa agagcgagat
    15541 ggtaatatca ccttttgtgg tactgagtac cgccttagtt gctttattgg gtgggaaatc
    15601 tgacagttgt ttcatagctg gggtattagg ttttactacg ttttgatttg cagctgcaga
    15661 atcgtttcca ttcattggag ttgttggaga gaccgctaac tgagtttgag tgattatagc
    15721 agtagtatct ggctggttat ttaggttatt gccacccgtt gttggagagg tgcatccaga
    15781 caaaaataat cctccagtaa tgagaaaaac tagctgatac gcatatttgt tgagagatat
    15841 ttgtttcatg gttgagtagt atacgcaaac tccataaaaa agtgaagcat gatacaattt
    15901 ttttgtgaaa gatacccaaa ttctaatttt acatggatgg acgagttcag atcaaacgaa
    15961 agataagtgg caaccactaa taaaactctt agaagagaaa aagtattctg tacgtttatt
    16021 attactccct ggtttgacgc agccactcag tgaaagttgg gatttagagc aatatgtaaa
    16081 gtggcttgat gctcaagttt cagtcaataa acaagtcatc attattggtc attcatttgg
    16141 tggccagttg gcaattaagt atgccgcgca tcatccagag catttgaaat gcctgttatt
    16201 gattgatagc tctggtgtgc cagataatcg ttggcagaaa aaactaaaac ggttagtctt
    16261 tttaaccctc gcaaaagttg gtaggaatct accacaaaag aaatttttaa aaaaattgct
    16321 gtatagattg gctggtgaac gagactacta tcaagctaca gaaaaacaaa aagccaccat
    16381 gcgtttagca attcgtacag atatcaggga agaagcgagt aaaataaaga ttccaaccct
    16441 actactttgg ggagaaaacg atcaaacaac accgatattt atgggaaaaa cgttagcaat
    16501 gctcatgcaa aatagtcaat tgcagataat tcccaacgct cgtcattctc ctcagtatac
    16561 acaccccatg caggttacat ccatatttca caattggctt caacaattaa atagtaatgc
    16621 ttctaaggtc taataatgat tctatttaag attactctca ccattgtagg tctaatacga
    16681 ttgcttcgtt ggctgagtat tattcaggaa aaggaatatc gttgggatcg actaaaaata
    16741 tttatatttt caagcaaggg cttgcgcgag ttagtgagag taattcctcg accaagcgac
    16801 tttaccaggt ccggcctcaa gcgacccaag agaactggtc gcgtgctcgt aattgctgtc
    16861 atagctatca gttacatcta caatcttatt caaattagcg acacgtgggt ggggcaacta
    16921 gactatcagt taggcgcaga tctgatgacc agtctttttt tactatcaat atatatttta
    16981 attcctacgt ttgtgttgtt ggcaattatt ccaacagttg ttattagtag ttttcaaact
    17041 aatctgactc ttttacgagc aaaaagaaaa ttgaatcaac agaaaccgat ggttattggt
    17101 attacaggtt catttggtaa aactgccacc aagcaattat tggcgcacat actaaacgaa
    17161 gttgagccag tatttgcaac tccaaaatca tttaatactc gatattcggt ggcgcgcagt
    17221 attttgaagg gttacaagtc agaaaaaatt gcgattttgg agtatggtgc gtatgcaaag
    17281 ggagaaattg ctaagcttgc atcctggttc caaccaacaa ttgcagtcat tacgggatta
    17341 acatcacagc atcttggatt atttggcaca cagcaagcga taatagaggc aaaatcagag
    17401 ctaataactt cattaccaag acaaggcgct gtggtgtaca acgcgttaga cgaaggtgtg
    17461 caacaaattg ttacgttggg tgtacataat tggcaaataa agcatcacac aaatcaggaa
    17521 ccgattctca taggttgtaa tccaaaaatg tccaaacaaa cgtttggata tgtattagaa
    17581 aaatctggac atttagcaat acttgcgggc aagcgaaaga tcatcaccga cttaattggt
    17641 atgcagtatg cccaaacagt ggagttggct gtgacaattg gagctcgatt gaagttatca
    17701 gaagcggcta ttttgcatgc catcagcact ttttcacctg gagataactt tattcaaatt
    17761 agaaaaacta aatcaggggg tacgattatt gatgatggcg gaactgctaa tccagctggt
    17821 tttaaagcag cccttgagtt gcttcagcag agtgaaaata aaaataaatg gctcatcact
    17881 cctggaattg tagatttggg attagaatca aaatctgtac atcaaaaact tgcagatttt
    17941 agcgcgtctt ttgtccaaaa agttctatat gttgatttga atggaagagc tgaatttgaa
    18001 gcaaaatttg gtacgaactg tgaaacagac agagaattga ttatcaagcg tttgaaacag
    18061 atggattcag atgtagtctt actcattgag ggtagaatgc ctggatgggt tcaaccatac
    18121 attaaagcat tatgaagcgt tttaacattt ttaattcact tggatcaaac tattcttggc
    18181 gtgatgcgcg tattgcgtta cgaggatttt tctcgccaga aaaatcctct cttgaacaac
    18241 taataactct ccttacgcaa gctttccctg gatattccgt tgtaccaaca tacaaagggc
    18301 gagacgcttt agaattatca cttcgatctt tgggcgtagg agtgggtgat gtggtgttaa
    18361 ctcaagcgtt tagctgttat gcagtagaag aggccattat tcgttgcggt gctaagccag
    18421 tctactacga tattgataga gctatgctag tgcccacaac agcaacttta gatcaagcgc
    18481 tgcatcgtca tgaggtagaa aattcaaatg gagtaaaagt tgcagcgatt attgctcagt
    18541 tcacattggg gaatatcagc tccagcaatg taattgtgaa ttggtcaaaa aaacaccaaa
    18601 tagcgtatgt ggcagatctt gctcaagcca ttggtgcaaa acaaaatgaa acgttactcg
    18661 gaaatggcgc agacgcaatt gtactttcta ctggtaggga taaagtttgg gatggtgtga
    18721 gtgggggatt tgctctgact agagatccga ttcatagctc actcttgccg agagatgcgg
    18781 tggggttaat tcaaaccatt cgtgactgcg tttatcccat actcaccata cttattcgca
    18841 aattgtaccc aatctatttg gggaaattat tacactttct tgtcacaaaa atgggtgtat
    18901 tttattctcc aatcgaaaca atttatcgtc gcacaacaca aatgcctcaa ggtttagcga
    18961 cctttctact tcatcgctgg cccactattg aatcagattt acctcacaga aaggtaattg
    19021 caaaagtgta ttataagtta ctcagtgaac acagcttaat tgatgaaaaa ggcatagagg
    19081 acggaacaaa cctgaggttt cctgtctatg tggaaaatcc tcgcgaaata atagacaaac
    19141 ttcaagaatt tggaatttat attgctgacc gttggtatcg taagcccgtt gatagtggct
    19201 cttttccgta taaaagtagc tatgaaccag gtagttgtcc caatgcagaa attattgcta
    19261 accattgcat caatttacca acccatcaac taattaatcc aaaaatagcc aagcaaatag
    19321 cagaactggt tttatgaaac ttacgcaatg taattcactg ggggaatgga acctatgtat
    19381 tagacatttt gccgaggcaa actttcttca gtcttggcaa tggggtgagt ttcaacaatc
    19441 tttaggtaaa gcagtatttc gatttgtcgt actgagtggc tcccagccaa ttgcccttgt
    19501 gcaggttgtg agagagccag ccaaaagagg cgcctatttt gcaattgctg ggggaccttt
    19561 acttgattgg aataatcatg aagtggtcac atttttgttt gctgagataa aaaaattggc
    19621 tctgcaacac aattgtgttt tcatacgttt tcgtccacag gttgaacaac ggcttgaact
    19681 aaggcaattg ttgcattcga ttggtgccgt aacggcgccg atgcatttaa ctgcagactt
    19741 aactattgaa atcgacttaa ccaaatccga tgaggaattg aaagctgaaa tgcgcaaaca
    19801 acatcgacaa gctttgaaaa aggcaactga attgggagta cacgtgcaat tatcaaaaaa
    19861 tatcaaggat cttaaacagt tctatcaaga acaattggtt gttgcgcagc gtcaaggttt
    19921 tgtacccttt tctgagagat ttctaacaaa gcaatttgaa gcttttcttg ctgatgatca
    19981 aatcgtattg tttcatgcat attatgaaaa gaccttattg gcatcagcat ttgtcatttt
    20041 ttataatggt gaagcagttt accactatgg aatttcaact tcagaaaata gggaataccc
    20101 aggtagctat gcttgtcagt gggcaattat tcaggaagca aaaaagcgtg gttgtagtag
    20161 atataatcta tggggggttt ctcccaagga tgcaatccac catcgttttt atggagtggg
    20221 tttgtttaaa cgtggttttg gaggcaagga agttgcctat gttccggctc aggacattcc
    20281 gctgaactgg aaatattggc tgacttggat ttttgagact ctacgtcgaa aaatgagaaa
    20341 gctgtgaatg attacagtcg ttctatacga tagctagtaa gatgctcgag aaaaattgct
    20401 gcagcgtctt cagtcagaaa ttgttggaac tcttgataga gttgaattcg ctcatctcga
    20461 ctattggttt gcctgccttt ttctagcaaa ttatctattc tggtattctt atagccagaa
    20521 aaattagttg gttcattgga gtgccataat tcatattgat ctggatcagg gggagcttct
    20581 tggccaatta gaatagtatc aaaagtggat gtatcaggaa aattggcgat ttttaactga
    20641 acttcgatgc ctaaatttgc acacaatgat ttatcttgga cttgactgga ttgattgcac
    20701 cgctcaacag ctagactacc gaggttttgc cactctgact gaatagttgt tgcctgctct
    20761 tcaaataagg tggtggtagt tagagaaaat gagagctttt ctctaggtag ttcctctaga
    20821 agtcgttcaa tggctcgctc aatatcagta tcatacgatt ttgaacctgc ataata
//
LOCUS       DAOQZP010000014        20231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_3022_length_20231_cov_5.901566, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000014 DAOQZP010000000
VERSION     DAOQZP010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 20231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20231
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3022_length_20231_cov_5.901566"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PKH60_01600"
     CDS             <1..633
                     /locus_tag="PKH60_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16777.1"
                     /translation="VQADISEAVRRFGGAGTHYGTESVGLLDEAALDVEIFDKPARKG
                     KIISMTDALGRMRAIRARGKRVAVTLGNFDVGTKGHMRVIREFVWATHNHGEVFVLVG
                     GDKEVAARKGPERPYVKHQQRMEFVAQQPGVDWVVPVSFPEFQNFAELQQNYERLHSD
                     LSGLAHCRLIGDVEATFPSYLAQCNRAGILLVHSTSPRISRATEEGSKTL"
     gene            complement(675..983)
                     /locus_tag="PKH60_01605"
     CDS             complement(675..983)
                     /locus_tag="PKH60_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16778.1"
                     /translation="MKQKEFSEDELKAILDLKLLSELDPLDFLRLAVLKILQEVDGLK
                     KSLVSGGGFDNPTKQALIEIGMKNADRVQSLAESLDPKNPKQIEELLKARASWNRKYE
                     "
     gene            complement(1061..1348)
                     /locus_tag="PKH60_01610"
     CDS             complement(1061..1348)
                     /locus_tag="PKH60_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16779.1"
                     /translation="MTDTLLLIGSLSNDLFRVASLTQRGSTKAASRFLLEAKRWSNSI
                     QDHTLPPYIQSIVKNVSDYKETLVDMETAEKYLMYAVLLQNYSLHQMENQT"
     gene            complement(1345..1683)
                     /locus_tag="PKH60_01615"
     CDS             complement(1345..1683)
                     /locus_tag="PKH60_01615"
                     /inference="COORDINATES: protein motif:HMM:NF039191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5674 family protein"
                     /protein_id="HNQ16780.1"
                     /translation="MIITKNKPFTIEEIELLKEEFEIYVKTVIDIENKVCSAGMNRHF
                     EGEQLLLQQDSKQKNIWGGGIDLETSEIDFNSFINIRPRDNNPKNEIQSEVIKAEYKK
                     LTEYFFQNVL"
     gene            1750..2832
                     /gene="ychF"
                     /locus_tag="PKH60_01620"
     CDS             1750..2832
                     /gene="ychF"
                     /locus_tag="PKH60_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNQ16781.1"
                     /translation="MSLSVGIVGLPNVGKSTLFNALLKRQVALAANYPFATIEPNVGV
                     VAVPDERLAVLAKVVGTTVLKPATIEFVDIAGLVKGAYKGEGLGNQFLSHIRETDLIC
                     HVVRAFRDEQILREDSTTPVDDLQTIRTELQLADLSTMERQSQPKGVKVAGAQERWQA
                     IEFFREKLGQGIMLNTLKLTEVQHETAKELCLLTVKPELFVYNEDEDQVVSSMEQLPK
                     IAKDLGVELEQIVVMSAKMESDLAALEETDQHAYLLELGLSLAGLERLAKVAYDTLGY
                     QSFLTAGVMEVRAWTITKGTTAQQAAGVIHTDFAKKFISARIVSYQDFVKLGGWKAAK
                     DIGKIRQEGRDYRMQEGDVVEFMIGS"
     gene            2877..7127
                     /locus_tag="PKH60_01625"
     CDS             2877..7127
                     /locus_tag="PKH60_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ16782.1"
                     /translation="MTESRFDPTNISPDFQQLTTVQDALVGTGNNVQLGPDFYDHQRT
                     QEAMTMQPSPDFGERHRPLGRTKELWAGQSREPLRPFVELIANACDSVVRVKQKEYGY
                     QLPRVEVRVTARKDGGYTILISDKGKVTGQHGEGMAVEYFSKIMKPFATGNEGNSETV
                     GMMGVGAQQRLNLLRNSADKIFVSSSTSQGILVSKEQLASDGENIDLRYGGVENEHTK
                     QLGTSFVIQLSAETAGEIGLTIPALSNYLKAKFGGSFDKDIALSSIDQTTKLAQKTVE
                     TVTPIESQFRRLNLNNDQTIQLAFSKEKTTIEAPICQVYLDVLGVSYGEPLEISRPGF
                     PKIIRLNMNQKIATITESRGKFGLTRLVVESVINAVRRFSQQFQTDTKTKVQLFDALC
                     PLFSRFADLRSLPVALRTDPTLNVSQTIKQSYLTVLAQASENLIVPSELADVYGDAEG
                     VIIVTDERVPSEYSYKLENWPNTSLVEEMRVNGKPVVVVDLPAGEQFRLVADRLFVSR
                     ESFHGLLSRDKAERRGCAIKLVAVLENHIDANPERKLTISYLLKTVEEKKVDRQTATT
                     TPSDEELNDRHYYANLALKEELRQRDISLCSRVELLDLIQRAFEIYNDNGKCYFLPGK
                     NMMGEPLKAGRINAYDAPDYTLRLIVSLLAREGSSYFFGIVITNLHPFYLDSTNCAIL
                     MQVAQQVPFLFKLSWLNHPPNMSWVQSVLEGRHQSFENYMSSNLGSEWRSEFPQIVTL
                     ADMTNSYGHYSYALEVFLNDKTLLSQWQVYSRNIFYSICELGLDSEKQAKKVLYNLPI
                     LMQVQGVQVNHEKLVLFGYYLNGQRQHQTAKLTQEEVKAFDKIIAWMNDDTEVEVRQP
                     TEDVADQGEPINPASLWILHQTQQLDMSRSIAEQTDFEPDREALRALLHPINRLQSDP
                     GTSFAEVVKNALDALSRDSTAKRQVRVTDYQVLDQQTKGWKYCVEVQDWVGIDPQEAL
                     VKLTLPQVGEGIHGLGFLKLLAEFDEIRVTTSNGVNTGEIVYTPIRSDDGQDTVVDFT
                     VRYRELETTRRGTKVKLTKKSTSPSVDAAIFRGSLHDFARQIPESAGQMYLNDQGEPL
                     NTALKTVARFNHSELGQVELTIRKEGAVEARSSPQVTVQRVEVRDFNLSSLIRSRLRS
                     LPPVQIELLATSDFSLTFDGVKTTTIRDGSGFANDHLVTPRIAQLIHNEADVIIAGLA
                     AEGALSCQNYLTTNFSPRLYDSPKEFVDKERELRTLAGGDENMQKMIDVVYTLPWKGD
                     DGRSYPSLLAWLLTQEKQRESSSTTSADEVKKVNYWEDIPHIVRRPLLYFHGVFAPDR
                     FFSFDGAEGSIPIYLTNKPGSRAFYRPRYGTLHYSTEVLEELTSSDPRTQARGIKTIA
                     HELQHVQEHTEEGLIHPPEFYTKVESKLLAIEDRMYAR"
     gene            7117..11619
                     /locus_tag="PKH60_01630"
     CDS             7117..11619
                     /locus_tag="PKH60_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16783.1"
                     /translation="MLAEDQSLTNNAELEAAYSPLELLSQKTDQRLQPLWDLVNRNFS
                     QSFEEEGVYQLADAVDQQSRLLYVQKATILIEKSSELLDRLEGDLDELEEQFRAAYHI
                     EWWDARGRLDKLNAESGLRRILSIIKTKKVQKQFDSVDARVETLQSSIDSKKTQIREL
                     QNQVSRLKVKRAEYFSQEVNDAVSQTVSGYSEFLNSLLSSTELAESLQEDLIQEKIVP
                     WLDSLSDTMQLSVEKRTTILESIRFYLRNLYLPNGDPQTYLLLVERELRELSAELPAQ
                     FRGIVELIRRKSTFEHVLCEVISRTATRSLSPLVKAARKDMYQGIATPPDIAGEIERA
                     INRGLADSTFSASRLLKDHFAEKMRLSYEYKPMSVTDVQLWSRLKEMDSAKSIFGAEM
                     VRQDEEQYSYVLENAISDSNGHIVDLLVYYPTAESIRNLLLLAAAGGSSYRTAHANRA
                     LQNLAKQPDWSTQVDELILVYPEFSQEVHLLKDWNLGGDTRYEVARLICGSYTRSVIE
                     SVNPDEKLLQLSYDAIPNSEAIEVLIQRNVLSIEESDKLRKARSILVDVERELFEARQ
                     RLPETKHVQSVFFKRMLDHLLVSLLKSSPDEIDEELLALKLSYLNYAEKIIENAQDYD
                     ALSYLSDSIVSTHSKLILSSPDILSHFTKIREICLFEIDLATDQRVGREVGIAARNRE
                     VSVSEYVKDISNYELKRIDEVLSSEDAEQIGSLSNIQLLIAYIRLNSENMMLRSLSEG
                     AVIKIKTMVEESRIADDVYLELIKIWNNYLNGDHASPPFSLIFFCEFIEACDGAGPLT
                     QIQALSKAIVTHIRSMNSDIPEDSKVATSKGLFAIEGRFQSERWSNDARTDFYSISQE
                     IQAIDPILYSEFFEVINHLSSAELKFFVQNVFPLFRTWLVMIEQRIEPSSRPTYDGLI
                     IEQIIKEIRNCANQVKQGTFNASEKRTEILHYIRALFKQRFGILSVPEDFSIEGARSL
                     SNVATYLTNLNDRTPKREAILAYYLALMLNNHWQEFRSGKPFPPEDLLTSEMVSQLQE
                     YKDALSEPKQLFDIEQLGIEAGELDDFVITLQAEASTITIGNIETVDAKLSNIVLNIK
                     ELEDLDFYTDTLDKERMRLLLSWGKKRIGSVVARLYQSLPPISKSISFSDEDAEIIGE
                     IKKLLIDSGRDVSQATVKEVFQDGMKPLSSVLSIIEIVSDFEVERELEKMQSLMKPPS
                     VVVAVFQRLGESFTLQSGASAYYPDLQYLENLIVKNEDQIANDEKSVVLDYIDAVREQ
                     LQKLDQIHQTMKKRLSGVILSESSLENQQLVEKMQEIFTTLETKTEAQPIISTVTNNL
                     NLVIENMRACLSATKNGSNNDTNLTFGDPTKFYVFSQAEMKKTGSIADEVVFFAPITY
                     EGGNGGGQHSEYAFVLDQIYGTHTTAVLNGHLRSLSNKVTAIKSKFPLAKISIFVPAS
                     TLKSSGTSEDSFETLLIENNLSGFQVEANVDIAESAAGDHYIEFGGESARRAGKRQAN
                     GYIIMV"
     gene            11658..12266
                     /locus_tag="PKH60_01635"
     CDS             11658..12266
                     /locus_tag="PKH60_01635"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNQ16784.1"
                     /translation="MFTLPNLNYKYDALEPFFDARTMEIHHTKHHQAYIDKLNDALKT
                     YPEWQHKTVEELLADLKSVPVPIRTAVRNHGGGHANHTLFWEWLSPDDQLTALASLTE
                     MFQPFGGFDTFKDQFAQVALGRFGSGWTWLVANDKKELSIISTANQDSPLSDGLTPLL
                     GLDVWEHAYYLKYQNRRADYIEAFWHVVNWGKVGELYRSAKK"
     gene            12357..12890
                     /locus_tag="PKH60_01640"
     CDS             12357..12890
                     /locus_tag="PKH60_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16785.1"
                     /translation="MKLNKLALGVLTLGLVGVFGLQQIGKPITVSAATSLTQVVRNTL
                     VTAVRDPFYPDYYHRVFVFPTAFAGWWFGDAYASNLHLITFTSLDPRLNEMSRSFYTL
                     GYSGGARGLELPIMNGTVPDSAYMYYPGSLTASDDFHFFFPASPSGELTSPVRVQMED
                     ILDRIVYVPARWWEPMR"
     gene            12995..13315
                     /gene="rpsF"
                     /locus_tag="PKH60_01645"
     CDS             12995..13315
                     /gene="rpsF"
                     /locus_tag="PKH60_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNQ16786.1"
                     /translation="MMKLVTDGRVRKYELTFLIPINFTTTEVSKLLEQIESLCKKHKT
                     EVVSKTEWGEQALAYAIRYKGKPLNSAVYHHWVLEAPAKQIAPLERALRLTPEIVRFL
                     LVVV"
     gene            13331..13879
                     /locus_tag="PKH60_01650"
     CDS             13331..13879
                     /locus_tag="PKH60_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNQ16787.1"
                     /translation="MSARSLNKVMLIGNLTRDPNLRYTPNNTAVCSFGLATNRSWTPA
                     DGGEKQESVEFHNVVAWSKLAEICGQLLHKGDKVYVDGRLQTRDWRGDDGQDRRTTEV
                     VIENMILLRSVGGQSEEGVGFESDEAQVAPRPARAKSKKASEDAGKSSDSAMPDDSSM
                     SAPVTTTDDIADVEDVADDVPF"
     gene            13945..14136
                     /gene="rpsR"
                     /locus_tag="PKH60_01655"
     CDS             13945..14136
                     /gene="rpsR"
                     /locus_tag="PKH60_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011057896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNQ16788.1"
                     /translation="MAKLPIGSELSYKNADLLASYVTEQGAILPRQVSGLTAKQQRQL
                     AREVKRARHLALLPFTQTV"
     gene            14176..15423
                     /locus_tag="PKH60_01660"
     CDS             14176..15423
                     /locus_tag="PKH60_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNQ16789.1"
                     /translation="MRFVLKLIPQLITAVLLVVVGMFAQYRYDLVQKTGPFLVQQTTV
                     GNTTGSRPVEAVSPSSVDMDTFWEAWSYVQADYLDPAKLDPQTMVDGATAGMVSALGD
                     PYTMYLPPEDNKRSSEDLAGAFFGVGIELGYKNEVLAVVSPLAGSPAEKAGIQPGDLI
                     LRVADDTNPKGEDSSKWSLNKAVDSIRGPKGSTVTLTLFRESNGAEPFEVALVRDEIV
                     VKSVKLEFVESQGKTFAHISVSRFGERTKAEWDDAVTQILTKKPVIAGIVLDLRNDPG
                     GFFDGAIQIASDFIPSGVIVSQKGKYTSQDFNSLGTGRLAKIKTEVLVNGGSASASEI
                     VAGALRDRIGSKLIGSKTFGKGTVQDRRELSNGGGLHVTIARWLLPGGDWIHDEGIPV
                     DIEVTVDPEATTDQVLERALKEF"
     gene            15452..16672
                     /locus_tag="PKH60_01665"
     CDS             15452..16672
                     /locus_tag="PKH60_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNQ16790.1"
                     /translation="MQENRSSFVNGVVVAGLVIGAYFLGTLSTRILNQDPVDLFGQLS
                     FLQNTPLSNWLPKNNAEPTTTPLGDLIKQGKELTIPDVVEAAGESVVTVAIKKQQTIV
                     DPFGGGLFNFGPFSFGNPALQRTEQIQRDIGTGFVVDSQGKVVTNKHVVSDTQAEYLV
                     VDKEGNEHKVEKIYRDPTNDLAIVQVADLKKPALPLGDSDTIRVGESVIAIGTALGEF
                     RHTVTTGVVSGLGRGITAGDGLSQFEEIEGVIQTDAAINPGNSGGPLINARGEVIGVN
                     VAVSQSAENIGFALPINVIKASLENFNATGQFERPMLGVRYQMITERAALTNEVPQGA
                     YVAEVVENSTAAKIGIQPGDIITMINGSKLKDTELAVILNKLKVGQSVKIEIWREGEF
                     KELEATLQSEVVNQ"
     gene            complement(16662..17618)
                     /locus_tag="PKH60_01670"
     CDS             complement(16662..17618)
                     /locus_tag="PKH60_01670"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HNQ16791.1"
                     /translation="MNPYQAQIDQLQVKLEAAKALAADPEYADLARAEIIELRTQIEL
                     LTQAASQLSENQTTDVEVDSTQKTNCTMEFRPGTGGDEAKIWASDLLRMYVRYLETTK
                     FRVSYIDDFVIKIVGKNVFENDTLSSNIILTPYEIFQYESGVHRVQRVPETEAQGRIH
                     TSTATVAVLPEVPPHVITIRDEDLEWQFIRASGAGGQHVNKTSSAVRLTHIPTGIATT
                     CQQEKRQEQNRKIALELLRAQLWEIEEEKRLAQLGEARSAIGQARRAEKIRTYNYPQN
                     RITDHRTKQSWHNLESIINGNLETLLLDLRGAMENLLIIPTD"
     gene            complement(17674..18060)
                     /locus_tag="PKH60_01675"
     CDS             complement(17674..18060)
                     /locus_tag="PKH60_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HNQ16792.1"
                     /translation="MKQNIHPTLNPIIFIDEDTKEEIFSKSTMTSDETREVDGVTYYV
                     VRLDVTSFSHPFFTGEMRFVDRQGRVDKFIQKMQNAQNTAGKKKKKLAKQADAAVEES
                     KSYREILQDQQSQLRKSAKPADAPKA"
     gene            complement(18131..18865)
                     /locus_tag="PKH60_01680"
     CDS             complement(18131..18865)
                     /locus_tag="PKH60_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16793.1"
                     /translation="MQNLLKERLVSALNQVHGTTEPVDPKERRRLLIGAGMLALGGVA
                     IAVLEACNIDPTPIKPELQVGRVERALIAMVEAALNKIVQPGTIFEGQTVVPIISVEL
                     PSDTNDPTDTALMLKMEIENSAQTIYIIPDLGGSDIENDGKQLVIRLRAHMWIADGER
                     LVYIENTKLRSRVLAGDGVTKISRLSPKDVFNKDLPTELEGKIFYPSNRQFKQYNFKM
                     DSNWEFNPNLNGWTRAVEFNPGTSLS"
     gene            19255..>20231
                     /locus_tag="PKH60_01685"
     CDS             19255..>20231
                     /locus_tag="PKH60_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HNQ16794.1"
                     /translation="MCGIFAYFGTKEADSIVRQGLQNLEYRGYDSWGIAVTDGSQVGM
                     VKAIGEVPKKLSDKQKIKGQIGLGHTRWATNGGVTQVNAHPHLAKSGEFAVVHNGIVE
                     NADELKSGLIKRGWRFQSETDTEVIVYLLEEALQKQQNLVLAVKAVSALLQGRNTFGI
                     LTKSGELLAMKKGSPLVVGRSKTLDEYFLSSDTASLVGLADQFLVLDDRQLVVVRKNG
                     VELPQLGLTNGKGETIQAQWEKLALDDQSSKLTEKHYMIQEILETPSVMKTLARQSKA
                     KYQLVARLIKQAKKVFVIGSGSTGLAAASIASHLRESGEIEAIALVGAEA"
BASE COUNT         5976 a         3725 c         4713 g         5817 t
ORIGIN      
        1 gtacaggctg atatctctga ggcagtgagg cggtttggtg gggctggcac gcattatggt
       61 actgagagtg tgggattgtt ggatgaagct gctttggatg tagagatttt tgataaacct
      121 gctaggaaag gaaaaataat ttcgatgact gatgcattgg gcaggatgcg tgcaatccgt
      181 gcgcgtggga aacgcgtggc agttactttg ggaaatttcg atgttgggac taaaggtcac
      241 atgcgagtga ttcgtgagtt tgtgtgggct actcataatc atggagaagt ctttgtgttg
      301 gttggcggcg acaaagaagt cgccgccaga aaaggccctg agaggccata tgttaagcac
      361 cagcaaagaa tggaatttgt tgcccaacaa cctggggttg attgggtagt gccagtttcg
      421 tttccagaat ttcaaaactt cgctgaacta cagcaaaact atgagagatt gcatagcgat
      481 ttaagcggac tggcccattg cagattaatc ggcgacgtgg aagcaacatt tcccagctat
      541 ttggcacagt gtaatcgagc ggggattttg ttagttcact caacttcgcc acgaatttct
      601 cgggcaactg aggaagggag caaaacgctc taacaatgat tgtttcacct tttcttttgt
      661 ctcgcttccc acctttattc gtattttctg ttccagcttg cgcgtgcttt aagcagttct
      721 tctatttgct tgggattttt gggatccaat gattcagcca gggattgcac tctatcggcg
      781 tttttcattc ctatttcaat tagggcttgt ttggtggggt tgtcaaagcc accaccagaa
      841 accaaacttt ttttcaagcc atcaacttcc tgcaaaattt ttaaaactgc taaacgaaga
      901 aaatcaaggg gatctaactc ggatagtaat ttcaagtcta gaattgcttt caattcgtct
      961 tcactaaatt ctttttgttt catatcaaac gaattatacc cgaaacaatt agaaagacaa
     1021 tttttacttt caagtactat tcacttgctg gattttagaa ttacgtttga ttttccattt
     1081 ggtgcaatga ataattttga agtaatactg catacattaa atatttttct gcggtttcca
     1141 tatcgacaag tgtttctttg taatcagaga cattttttac gattgactga atatacggag
     1201 ggagtgtgtg gtcttggatt gaatttgacc agcgttttgc ctctagtaaa aaacgagacg
     1261 cagctttggt tgatccgcgt tgagtgagag aggctactcg aaaaagatcg ttggaaagtg
     1321 agccgattag taacagggta tccgtcatag tacgttttga aagaaatact cggttagttt
     1381 tttgtattca gctttaatca cttcgctctg gatttcattt ttaggattat tgtctctagg
     1441 tcttatgttt atgaaggaat tgaagtcaat ctctgaagtt tctagatcaa tgccacctcc
     1501 ccagatgttt ttttgctttg aatcttgttg gagtaagagt tgttcacctt caaaatgtct
     1561 gttcattcca gctgaacaaa ctttattttc tatgtcgatc actgttttta catagatttc
     1621 aaattcttct ttaagcaact caatttcttc aatagtgaat ggtttatttt ttgtgatgat
     1681 catcgtgctg ataatagcaa attgtgttgt cttgcgctga atcaattttt ctcaggtata
     1741 ctacagagta tgtctttatc agtcggaatt gttggtttac caaatgtggg gaagtccacg
     1801 ctgtttaatg cgctgttgaa acgccaggtt gcgttagcag ctaactatcc gtttgccacg
     1861 attgaaccaa atgttggagt agtggcggtg ccggatgagc ggttagcagt gttggctaaa
     1921 gtggtgggaa cgacggtgct caagccggcc accattgaat ttgtggatat cgccggctta
     1981 gtgaagggcg cgtacaaggg tgagggcttg gggaaccaat tcttgagtca catcagagaa
     2041 accgacttga tttgccacgt ggtgcgcgct ttccgagatg agcaaatcct gcgggaggat
     2101 tccacgacac cggttgatga cttgcagacg attcgcacag aattgcaact tgccgatttg
     2161 agtacgatgg aaagacaatc acaacccaag ggtgtcaaag tcgctggagc tcaggagcgt
     2221 tggcaagcaa ttgagttttt ccgcgaaaaa ttaggacagg gaattatgct taacacactt
     2281 aaattgaccg aagttcagca tgagacggct aaagaactct gcttgttgac agtgaagcct
     2341 gagctgtttg tgtacaacga agacgaagat caagtggtca gctcgatgga gcagctacct
     2401 aaaattgcga aagatttggg agttgagctg gaacaaattg tagtgatgag cgccaagatg
     2461 gagagcgacc tagctgctct tgaggaaact gatcagcacg cttacttgtt ggaactggga
     2521 ttatccttgg ctgggctgga gcgactcgcc aaagtggcct acgatacatt aggctaccaa
     2581 agtttcttaa ccgctggcgt aatggaggtg cgagcctgga cgattaccaa gggcacaact
     2641 gctcagcagg cggcgggcgt gattcatacg gattttgcta aaaagtttat ttcggctcgc
     2701 atagtttcat accaagactt tgttaaactt ggcggctgga aagccgccaa agacatcggc
     2761 aaaatccgcc aagaaggtcg tgattaccgt atgcaagaag gcgacgtggt ggagtttatg
     2821 attgggagct agccatccaa gctctagtac ctcttgattt atgctacgat ggtatcgtga
     2881 ccgaatcaag atttgatcca acgaatattt ctccagattt tcaacaattg actaccgtac
     2941 aagatgcttt ggtgggcact ggaaataatg ttcagcttgg tccggatttt tacgaccatc
     3001 aacgtacgca agaagcaatg acaatgcagc caagtccaga ctttggagaa cggcacagac
     3061 ctttgggcag aacgaaagaa ctttgggctg gtcaatctag agagccctta cgaccatttg
     3121 tggaacttat tgccaatgct tgtgattcag ttgttagagt aaaacaaaaa gagtatggat
     3181 atcaacttcc aagagtggaa gtgcgtgtga ctgctcgaaa agatggggga tacaccatac
     3241 tgatttctga taagggcaaa gttacagggc agcatggtga gggcatggcg gtggaatatt
     3301 tttcaaaaat tatgaagccg tttgctaccg gcaacgaagg taatagtgaa acagtgggta
     3361 tgatgggcgt tggggctcag cagcgactaa acttgctccg taattcagcg gacaagatat
     3421 ttgtttcgag ctcgactagt caaggaatat tggtttccaa agaacaactg gcaagcgatg
     3481 gggaaaatat tgatctgaga tatgggggag tagaaaatga acataccaaa caacttggta
     3541 cttcattcgt gattcaactc tctgcggaga ctgcgggaga aattgggctg actattccag
     3601 cgctctcaaa ctacctaaaa gctaagtttg gtgggtcatt tgacaaggac atcgctctgt
     3661 cctcaattga ccaaacgact aaattggcgc agaaaacggt cgaaactgtt actcccatag
     3721 aaagtcaatt taggcgatta aatctcaata atgatcagac aattcagctg gcgttttcca
     3781 aagagaaaac cacgattgaa gcaccaattt gtcaagtcta tttagatgta ttgggtgttt
     3841 catatggaga gccgctagaa attagtaggc caggatttcc caaaattatt cgcctaaaca
     3901 tgaatcaaaa aatagctacg attactgaaa gcaggggtaa atttggtctc actcgtttgg
     3961 tagtcgagtc tgtgatcaac gcggtcagac gattttctca acaatttcaa acagacacaa
     4021 aaactaaggt acagctattt gatgcactct gcccactgtt tagtcgtttt gcagacttgc
     4081 gatcattacc ggtggcgtta cgcactgatc ctactctcaa cgtaagtcaa acaatcaagc
     4141 aatcatatct aactgtttta gctcaggcat cggaaaacct cattgttcca agtgagttag
     4201 ctgatgtata tggtgatgca gaaggtgtaa ttatagttac agacgaaagg gtcccaagcg
     4261 aatattcgta taagctcgaa aactggccca atacaagcct ggttgaagag atgcgagtca
     4321 atggtaaacc agtagtagtg gttgatttac cagcgggaga gcagtttaga ctggttgctg
     4381 atcgattatt tgtatcacgc gaatcttttc atggcttgct cagccgggat aaagcggaaa
     4441 gacggggttg cgctattaag ttggtagcgg ttttggagaa tcacatcgat gcaaatccgg
     4501 aaagaaagct aacgatcagt tatctactaa aaactgtcga agaaaaaaaa gtcgacaggc
     4561 aaactgcgac cacaacgccc agtgatgagg aactaaatga tcgacactat tatgcaaact
     4621 tggctctgaa agaagaacta cggcaaagag atatttcttt gtgctctcgg gttgagttgt
     4681 tggatctcat tcagagagca tttgaaatct ataatgacaa cggaaagtgt tattttttgc
     4741 ctggaaagaa tatgatgggt gaacctctta aagcaggcag aattaatgca tatgatgctc
     4801 cagattacac actccgattg atcgtttcat tgcttgccag agagggttct agctattttt
     4861 ttgggattgt aattacgaac cttcatcctt tctatcttga ttcaaccaac tgtgccatat
     4921 taatgcaagt ggcgcagcaa gtgccatttt tgtttaagtt gagttggtta aatcatccac
     4981 ccaatatgtc gtgggtacaa tctgtcttag agggcaggca ccaaagcttt gagaattaca
     5041 tgtcaagcaa tttgggttct gaatggagat cagaatttcc tcaaatagta acacttgcag
     5101 atatgactaa tagttatggt cattattcat atgctcttga agtatttctc aatgacaaaa
     5161 ccttacttag tcaatggcaa gtgtattctc gtaatatctt ttattcgatc tgcgaacttg
     5221 gtttggactc cgaaaaacaa gcaaaaaaag tgttatataa ccttcctata ttaatgcaag
     5281 tgcagggtgt tcaggtaaat cacgaaaaac tagtgttgtt tggttactac ttgaatggtc
     5341 aacgtcaaca tcaaacagct aaactaactc aagaagaagt taaagcgttt gacaaaatta
     5401 ttgcctggat gaacgatgac acagaggtag aagtccgtca gcccacagaa gatgttgctg
     5461 accagggaga gccaatcaac cccgcatcgc tctggatttt gcatcaaaca caacagctgg
     5521 atatgagtag gagcattgca gaacagacgg attttgagcc agatagggaa gcgttgcgag
     5581 ctttgttgca tccaattaat cgactccaat ctgatcccgg taccagtttt gcagaggtag
     5641 taaaaaatgc actagatgcg ctatctagag attcaactgc aaaacgacaa gtgcgagtga
     5701 ctgactatca ggtattggac cagcagacga agggatggaa gtactgtgta gaggttcaag
     5761 attgggtggg aatagatcct caggaagcac tagttaaatt aacactgcca caagtgggcg
     5821 agggtattca tggtcttggg tttctgaagt tgctcgccga atttgatgaa atccgagtaa
     5881 ctacttccaa tggtgtcaat actggggaaa ttgtgtatac acccattcgt tctgacgatg
     5941 gtcaggatac cgtagttgac tttacggtgc ggtataggga acttgagaca acacgaagag
     6001 gtacgaaggt gaagttgacc aaaaagtcaa catctccaag tgttgatgct gcgattttta
     6061 gggggagttt acatgatttt gctcgccaaa ttccggaaag cgctggtcag atgtacctaa
     6121 acgaccaagg tgaaccactc aatactgcat taaaaacagt ggctcgtttt aatcatagtg
     6181 agttgggcca agttgaattg actattagaa aagaaggtgc ggtagaagct cgttccagtc
     6241 cgcaagtgac ggtacagcga gtggaagtga gggattttaa tctcagctct ctgattagat
     6301 ccagactgcg atcattgccc cccgtgcaaa ttgaacttct tgcaacgagt gatttttcat
     6361 taacatttga tggtgttaaa acaaccacca ttagagatgg tagcggtttt gctaatgacc
     6421 atttggtcac ccccagaatc gctcaattaa ttcacaacga agctgatgtg atcattgcag
     6481 gcctggcagc agagggtgca ttgagttgcc aaaattactt gacgactaat tttagtcccc
     6541 ggttgtacga ttctcccaag gagtttgtgg ataaagaacg ggaactacgt acacttgcgg
     6601 gtggtgatga aaatatgcaa aaaatgatag atgttgtata cacacttccc tggaagggtg
     6661 atgatggaag gtcatacccg agtttattag cttggttgtt aactcaagaa aagcaacgag
     6721 agtcctcttc gacaacgagt gctgacgaag taaagaaagt aaattattgg gaagatattc
     6781 cacatatagt gcgacggccg ttattatatt ttcatggtgt ttttgcccca gaccgcttct
     6841 tctcttttga tggggctgaa ggatcaattc caatctacct cacaaacaaa cctggctcac
     6901 gcgcatttta tcggccacga tatggtactt tacattattc aacagaagta ttggaagagc
     6961 tcacatcttc agatccaaga acccaagcga gaggaattaa gacaattgca catgaactac
     7021 aacacgtaca agaacacacg gaagaaggct tgattcaccc tccagaattt tatacaaaag
     7081 tagaaagcaa actactagcc atagaggatc ggatgtatgc tcgctgaaga ccaaagtttg
     7141 actaataatg ctgagttaga agcggcgtat tcacctttgg aattgctgag tcaaaaaact
     7201 gaccaacgtc ttcaaccgct ttgggattta gttaatagaa atttttctca aagttttgaa
     7261 gaagaaggtg tttatcaact agcggatgct gtggatcaac agtctagatt gctgtacgtg
     7321 caaaaagcaa caattttaat tgaaaaatct agcgaattat tagaccggtt ggaaggagat
     7381 ttagatgaac tagaagaaca atttagagcg gcataccata tcgaatggtg ggatgcgaga
     7441 ggaaggcttg ataaacttaa tgcagaaagt ggtttacgac gtattttatc cattataaaa
     7501 acaaaaaaag tccaaaaaca attcgactca gttgatgcaa gggttgaaac tttgcagtca
     7561 agcattgata gtaaaaaaac tcaaatcagg gaactccaga atcaagtatc tcgattgaaa
     7621 gtaaaaaggg cagagtattt ttcacaagaa gtgaatgatg ctgtcagcca aacagtatct
     7681 ggatattctg aattccttaa ttcattactt tcttcaacag agcttgctga atcacttcaa
     7741 gaagatttga ttcaggaaaa aatcgtacca tggcttgatt ctctatcaga tacaatgcaa
     7801 ctatcggttg aaaaaagaac gactattctt gaatcaattc gtttttatct acgcaatctg
     7861 tatcttccca atggtgaccc gcagacatat ttgcttctag ttgaacgaga attacgggaa
     7921 ctctctgctg aacttccagc tcagtttcga ggtatagttg aattgatcag aagaaaaagt
     7981 actttcgagc atgtgctctg cgaggtcatt tcacgcacgg caacaagaag tctttctcca
     8041 ttagtaaagg ctgctcgaaa agatatgtac cagggaattg ctactcctcc agacattgct
     8101 ggtgaaattg aaagggccat caaccgtggt ttggcagatt ctacgttttc cgcttctaga
     8161 ctactaaaag atcattttgc agagaaaatg cgcttgagct atgaatataa accaatgagt
     8221 gttaccgatg tgcagctttg gtcaagactc aaggagatgg attcagcaaa atcaattttt
     8281 ggagctgaaa tggttaggca agatgaggag caatattcct atgtgcttga gaatgcgatt
     8341 tctgattcta acgggcacat cgttgatttg ttggtgtatt accccaccgc agagtcaatt
     8401 agaaacttat tacttttagc tgcggctggt ggtagttcct accgaaccgc acatgcaaat
     8461 agggctttgc agaatctagc aaaacaacca gattggtcaa ctcaggtcga tgagctgatt
     8521 ttggtgtatc cagaatttag tcaagaggta catctattaa aagattggaa tctaggtggc
     8581 gatacaagat atgaagtagc tcggttgatt tgtggttcgt atacacgctc agttatagaa
     8641 tcagtaaatc cggacgaaaa actactacaa ttatcatacg atgccattcc aaactcagaa
     8701 gcaattgaag tattgattca aagaaatgtt ttatcaattg aagaatcaga taagctgcgg
     8761 aaggctagaa gcatattggt tgacgtggaa agagaacttt ttgaggcaag acagcgactt
     8821 cccgagacca aacatgtgca gtctgtgttt tttaagagaa tgttggatca tctacttgtt
     8881 agtttgttga aaagttcgcc agatgaaatt gatgaagaac tattagcctt aaaattaagc
     8941 tatttgaatt atgcagaaaa aatcattgaa aatgctcagg actatgatgc gctttcgtat
     9001 ttgtccgatt ctatcgtaag cacgcattcc aaactgattc tcagtagccc cgatattctt
     9061 agtcatttta caaaaattcg agaaatttgc ctttttgaaa ttgatttggc aacagatcag
     9121 agagttggac gggaagttgg gattgctgct agaaatcgag aagtttcagt gtctgagtat
     9181 gtgaaagaca tatcaaatta tgagttaaag agaatagatg aggttttatc atccgaagat
     9241 gcggagcaaa ttggttctct ttcaaatatt caactactga ttgcatatat aagattgaac
     9301 tcagagaaca tgatgctcag atctttatct gaaggtgcgg tcatcaagat aaaaacaatg
     9361 gtagaagaaa gccgtattgc agacgatgta tatcttgaat taattaaaat ctggaacaac
     9421 tatttgaatg gcgatcatgc tagtccgcct ttttccctta tattcttttg tgaatttatt
     9481 gaagcctgtg acggtgctgg gccgttgact cagattcaag ctttatctaa agccatagtt
     9541 acccatattc gctcgatgaa ttctgatata cctgaagatt ctaaggtagc aacaagcaaa
     9601 ggtctgtttg ctatcgaagg tcgatttcaa tctgaaagat ggtcaaacga tgctcgaact
     9661 gatttctact ccatatctca agaaatccaa gcaattgatc caattctgta ttctgaattt
     9721 tttgaagtaa tcaaccattt aagttctgca gaacttaagt tttttgttca aaatgtattt
     9781 cctcttttta ggacttggct tgtgatgatt gaacagagga ttgagccctc ttctagacca
     9841 acatacgatg gtctgataat cgagcaaata atcaaggaaa taagaaattg tgccaatcag
     9901 gtaaaacagg gcacttttaa cgcaagtgaa aaaagaactg aaattttaca ttatattcga
     9961 gcattattta aacaaagatt tggaatctta tcagtgccag aagattttag cattgaaggg
    10021 gctcgatcac tgagtaatgt tgcaacatac ctaaccaatc ttaatgaccg gacgccaaag
    10081 cgagaggcta tacttgctta ctatttggca cttatgttga ataatcattg gcaagaattt
    10141 cgttccggaa aaccatttcc tccagaagat ttattaacat cagaaatggt ctctcaacta
    10201 caggagtata aggatgcatt gagtgaacca aaacaattat ttgatatcga acaattggga
    10261 attgaagccg gtgaactaga cgatttcgtt ataactctcc aagctgaagc ttctactatt
    10321 acaattggta atatagagac ggtagatgcg aagctctcaa atattgtttt aaatattaaa
    10381 gagttagaag acctagattt ttatacggat acattagata aggaaaggat gaggttgctt
    10441 ttgagctggg gaaagaaaag aattggttct gtagtagctc ggttgtacca gtctttgcca
    10501 ccaatatcta aatcaatatc attttctgat gaagatgctg aaataatcgg agaaataaaa
    10561 aaacttctta ttgattctgg aagggatgta agtcaagcaa ccgtgaaaga agtttttcaa
    10621 gatgggatga agcctctcag ctcagttttg tcgataattg aaatcgttag cgattttgag
    10681 gtcgagaggg aacttgaaaa aatgcagtca ttaatgaaac ccccatcagt tgtggttgct
    10741 gtatttcaaa gattgggaga aagctttacg ctacaatctg gtgctagtgc ttattatcct
    10801 gacttgcagt atttagaaaa tttaatcgta aaaaatgaag atcagatagc aaatgatgaa
    10861 aaatctgtag ttcttgatta tattgatgct gttagagagc aattgcaaaa acttgatcaa
    10921 attcatcaaa ctatgaagaa gcgcctgagt ggagtcatcc ttagtgaaag tagtcttgag
    10981 aatcagcagc tggttgaaaa aatgcaagaa atatttacta ctcttgaaac aaaaactgaa
    11041 gcccagccta ttatcagcac tgttacaaat aacttaaatt tagtcattga aaatatgaga
    11101 gcatgtttgt ctgcaacaaa aaatgggagc aataacgata caaatctaac ttttggagat
    11161 cctacgaaat tctacgtttt tagtcaggca gagatgaaaa aaactggtag tatcgcagat
    11221 gaagttgttt tttttgctcc aattacttat gaagggggaa atggtggtgg acaacactcg
    11281 gagtatgctt ttgtattgga tcaaatttat ggaacacata ctacagcagt gcttaatggt
    11341 catttaagat cgttaagtaa taaagtgact gcgatcaaga gcaagtttcc gttggcaaaa
    11401 atctccattt ttgttccagc aagcactctg aaatcttcgg gtacctcaga agattcgttt
    11461 gaaactcttt tgattgagaa taacttatca ggttttcagg tggaagcaaa tgtagacatt
    11521 gcagaatcgg ctgccggaga ccattatata gaatttggag gagaatcagc gcgcagggct
    11581 ggtaaaagac aggcgaatgg ttacattatt atggtataac catattgtag aaagtagcga
    11641 cgctgtagga gttcgttatg tttactcttc ctaatctcaa ttacaaatac gatgccttgg
    11701 agccattttt tgatgcgagg acgatggaga tccatcacac gaagcatcat caggcatata
    11761 tagataaatt aaatgatgca ctcaagacat atccagaatg gcaacacaaa acagttgagg
    11821 agttgctcgc agatttgaaa tccgtacccg taccaattcg cactgcagtg cgcaatcatg
    11881 gtgggggaca cgctaaccac acattatttt gggaatggct ttcacctgat gatcaactca
    11941 ccgcattagc tagtctgact gaaatgtttc aaccatttgg tggctttgat actttcaaag
    12001 atcaatttgc ccaagttgcc ctaggtagat ttggctctgg ctggacgtgg ttagtagcta
    12061 acgataaaaa agaattgtcg attatttcca cagctaatca agattctcca ctgagcgatg
    12121 gtcttacacc actgttgggc ttagatgttt gggaacatgc ctactatctt aaataccaaa
    12181 accgtcgcgc tgattatatt gaagcatttt ggcatgtggt gaactggggt aaggtgggtg
    12241 agttgtatcg tagcgcaaaa aaatagtagt gtaatttgat gctgtactgt gtactcagtg
    12301 tgtagtacag tgagtttagt aacatctgta aatagtctta aaaagagggg agagtaatga
    12361 aactaaacaa attagcactt ggggtattga ctcttggatt agtgggagtt tttggtctcc
    12421 agcaaatcgg gaaacccatt actgtttcag ccgctaccag tctcacgcaa gttgtcagaa
    12481 atactttggt gactgctgtt cgagacccat tttatccaga ctattatcat cgtgtatttg
    12541 ttttcccaac agcatttgcc ggttggtggt ttggtgatgc gtatgcctca aatcttcatt
    12601 taattacttt tacaagtctt gatcctagat taaatgaaat gtccagatcg ttttacacat
    12661 tgggctatag tggtggcgct cgtggtcttg agttaccgat tatgaatggt acagttccag
    12721 atagtgctta tatgtactat cctggatcgc ttacagcatc agatgatttt cactttttct
    12781 ttcccgcaag tccttcgggt gaattaactt ctccagttag ggttcaaatg gaagatattt
    12841 tagatagaat tgtgtatgtt cccgctcgct ggtgggaacc aatgcggtag tcgtgtgcta
    12901 taatcaccac caattgtaat tagtagcgct ggcgttccca ttaaccgagt gttgatggtc
    12961 caaccgctca tcgaagccag caaggagcat gtgtatgatg aaactagtaa ctgatggtcg
    13021 tgttcgcaag tacgaattaa cctttttgat tcctattaat tttactacga ctgaagtaag
    13081 taaattgcta gaacaaattg agtctttgtg caaaaaacac aaaactgaag tagtttctaa
    13141 aacagaatgg ggtgaacaag ccttagctta tgctattcgc tacaaaggca aaccactcaa
    13201 ttcggcggtc taccatcact gggtactaga agccccagcc aaacaaattg cccctcttga
    13261 gagagcgtta cgactgacac cagaaattgt acgattttta cttgtagttg tgtgataaga
    13321 aaggaagctt atgtccgcac gatcgttaaa taaagtgatg ctgattggta atttaaccag
    13381 agatccaaat ttacggtata ctcccaataa tactgcagtt tgttcgtttg gattggctac
    13441 caatcgtagt tggacgccag ctgatggagg cgaaaagcaa gaaagtgttg aatttcacaa
    13501 tgtcgtagcc tggtcgaaat tggcagagat ttgtggccaa ctcttgcaca aaggcgacaa
    13561 agtctatgtg gatggtcgtc tgcaaactag agattggcgt ggcgatgatg gtcaagatag
    13621 acgaaccacc gaagttgtaa ttgaaaatat gattctacta cgatcagttg gtggtcagtc
    13681 agaagaagga gttggttttg aaagtgatga ggctcaggta gctccacgac ccgcaagggc
    13741 aaagtctaaa aaagccagtg aagatgctgg aaaaagcagt gactcagcaa tgcctgatga
    13801 ttcaagcatg agtgcgccag tcacaaccac tgatgatatc gccgatgtag aagatgtcgc
    13861 cgacgacgtg ccattttagt ttgtaaatag aaggaaataa ccatgccaaa atcaatgaaa
    13921 cagcgaaaag aaaagcgacc aacaatggca aaattgccaa ttggtagcga gttgagttac
    13981 aaaaacgccg atctgcttgc gtcctacgtt acagagcagg gagcaatttt gcctcgtcag
    14041 gtgtctggtt taaccgccaa acaacaacga cagctcgccc gtgaggtcaa gcgggctcgc
    14101 cacttagcgc tcctaccttt cacgcaaacg gtctaagccc ttgccattct cgtttctgta
    14161 ggtacaattg agcccatgcg ttttgttttg aagctgattc cacaactcat taccgcggtc
    14221 ttgctggtag ttgtgggtat gtttgctcag taccggtacg atttggtaca aaaaactggc
    14281 cccttcctgg ttcagcaaac tactgttgga aatacaactg gctccaggcc agttgaggct
    14341 gtttcaccat cgtcggtaga catggatacg ttttgggaag cttggtcata tgtgcaggct
    14401 gattatctag atcctgccaa acttgatcct caaacaatgg tggacggtgc caccgcaggt
    14461 atggtatctg cgcttggaga tccttacacc atgtatttgc ctccagagga taacaagcgg
    14521 agtagcgaag acttagcggg tgcatttttt ggcgttggga ttgaactggg atataaaaat
    14581 gaagttttag cggtagtttc accattggcg ggttctcctg cagaaaaggc aggtattcag
    14641 ccgggtgact taattttacg cgtcgccgat gacacaaatc caaagggtga agattcgagt
    14701 aaatggtcgc tcaataaggc cgtcgactct attcgaggtc caaaaggtag caccgttact
    14761 ctcactctct ttcgagaaag caatggcgct gaaccatttg aagttgcatt ggtgagagat
    14821 gaaatcgtag tcaaatccgt caagcttgaa tttgtggaaa gtcagggtaa aacatttgcc
    14881 catatcagcg tgagtcggtt tggtgagcgc accaaagccg agtgggatga tgcggttacg
    14941 cagattttaa caaaaaaacc tgttatagct ggtatagtgc ttgatcttcg caacgatcca
    15001 ggtggctttt ttgatggtgc aattcaaata gcgagtgatt ttattccttc tggcgtaatt
    15061 gttagtcaaa agggtaaata caccagtcaa gattttaact ctctcggtac gggcaggctg
    15121 gcgaaaatta aaactgaagt cttagttaat ggcgggagcg ccagcgcttc agagattgtc
    15181 gctggcgcgc tccgtgacag aattggtagt aagctcattg gtagtaaaac ctttggtaaa
    15241 ggtacggttc aggatagacg agaactctca aatggtgggg gcttacatgt aaccattgct
    15301 cgttggttgt tacctggcgg cgactggatt catgatgaag gaattccagt tgatattgaa
    15361 gtaaccgtgg atcctgaggc aacaactgac caagtgcttg agcgagccct gaaagaattt
    15421 taaatagaaa atcgtataac agctatattc tatgcaagaa aatagatctt catttgtaaa
    15481 tggtgttgta gttgctggat tagttattgg agcctatttt ttaggcacgc tttcgactag
    15541 aattttgaat caagatccag ttgatttatt tggtcaatta tcgttcttgc aaaatactcc
    15601 gctcagtaat tggttgccaa aaaacaatgc cgagcccaca acaactccac taggtgattt
    15661 aattaaacag ggcaaagagt tgactattcc ggatgttgta gaagctgctg gcgaatctgt
    15721 ggtaacggtg gcaattaaaa agcaacaaac catcgttgat ccgttcggtg gtgggctttt
    15781 taactttggc ccgtttagtt ttggcaatcc agcgctacaa agaactgaac aaattcaacg
    15841 agatattggc actggctttg tggttgattc gcaaggcaag gtggtcacca acaaacacgt
    15901 ggtttctgat actcaggcag aatatttagt ggtcgataaa gagggtaatg agcacaaggt
    15961 agaaaaaata tatcgagatc ccacaaatga tttagcgatc gttcaagtgg ccgacctaaa
    16021 aaaacctgcg ttgcctttgg gcgattcgga cacaattaga gtaggagaga gtgtgatagc
    16081 tattggtacc gcacttggtg agtttcgtca cactgtgacg actggagtcg tctctggtct
    16141 tggtcgaggc attactgctg gcgatggttt atcacagttt gaagaaattg aaggagtgat
    16201 tcagaccgat gccgctatta atccgggtaa ttcgggtggg ccccttatta acgctcgagg
    16261 cgaagtcatc ggtgtcaacg tagcggtgtc tcaatctgcg gagaacattg gttttgcctt
    16321 gccaattaat gtgattaaag cttcacttga aaactttaat gcaacgggtc aatttgagcg
    16381 tccaatgttg ggagtgcgct accagatgat cacggaaagg gcggctctga ctaatgaagt
    16441 gccccaggga gcgtatgtgg cagaagtggt agaaaatagt accgcagcta aaattggcat
    16501 tcagcctggg gatatcatta ccatgataaa tggttcaaaa ttgaaagata ctgagttggc
    16561 tgttatatta aataaattga aagttggcca aagtgtaaaa atcgaaattt ggcgcgaagg
    16621 ggagtttaaa gaattagaag ccactctgca aagtgaagtc gttaatcagt aggaatgatt
    16681 aggagatttt ccattgcgcc tctcaaatcc agtaaaagcg tctctaaatt accattaatg
    16741 attgattcta ggttgtgcca gctctgttta gtcctatgat cggtaattcg gttttgagga
    16801 tagttgtaag tacggatttt ttcggcgcga cgggcttggc caatggcgct gcgagcttcg
    16861 ccaagttggg cgagtctttt ttcttcttca atttcccaaa gttgagcccg gagcagctcg
    16921 agggcaattt ttcgattctg ttcttggcgt ttttcctgtt ggcaggtggt ggcaattcct
    16981 gtggggatgt gtgttaggcg cacggcacta ctggttttgt tgacatgctg gcctcccgcg
    17041 ccgctggcgc gaatgaattg ccactctaaa tcttcatctc gaatagtaat gacatgaggg
    17101 ggaacttctg gcaacactgc taccgtcgca gtggaagtat gaattcgacc ctgagcttcg
    17161 gtttctggta ctcgctgtac tcgatgcact cccgactcat attgaaaaat ctcatatgga
    17221 gtaagaatga tattagaaga taaagtatcg ttttcgaata catttttacc aactatttta
    17281 atgacaaaat catcaatata ggaaacccga aacttagtgg tctctaggta gcgtacgtac
    17341 atgcgtaata aatcactagc ccaaattttt gcttcgtcgc caccggttcc gggacggaac
    17401 tccatagtgc aattggtttt ttgtgtagaa tcaacttcga catcagtagt ttgattttcg
    17461 cttagttgtg aagcggcttg agtaagtaat tctatttgag tcctcagttc tataatctca
    17521 gcccgagcta gatctgcata ttcaggatca gcagctaacg ctttagctgc ctcgagtttt
    17581 acttgaagtt ggtcgatttg tgcttgataa ggattcatga ttagcactat tgtaccgcac
    17641 tagagataca cagagttagt tgaagcgtga aagttaagct ttgggagcgt ctgcaggttt
    17701 tgcgcttttg cgaagttgag actgttgatc ttgtaaaatt tctcgataac ttttgctttc
    17761 ttcaaccgcg gcatcagctt gtttggctag tttctttttc tttttgccag cggtattttg
    17821 agcattctgc atcttttgta taaatttatc aactctacct tgacgatcta caaaacgcat
    17881 ttcaccagta aagaaaggat gtgaaaacga ggtaacatca agtcggacga cgtagtaggt
    17941 gacaccatca acttcgcgag tttcatcact cgtcatggtg gatttactaa aaatttcttc
    18001 ttttgtatct tcatcaataa agatgattgg attaagggtg gggtgtatgt tttgtttcat
    18061 agtggctcca agagggagta gtatagcata aaaatttcta tcaagctgtc acaagaaagt
    18121 aaaggaccat ttatgataaa ctggtgccag gattaaactc cacagctcgt gtccacccat
    18181 tcaaatttgg attaaattcc cagttactgt ccattttgaa attatactgt ttgaactgcc
    18241 tattactcgg ataaaatatc ttgccctcta attcagtggg caagtccttg ttaaaaacat
    18301 ctttgggaga aagtctgcta attttggtaa caccgtctcc tgctaatact cgtgacctta
    18361 gctttgtatt ttcaatatag actaaccgtt caccatcggc aatccacatg tgggccctta
    18421 atcgaattac caattgtttg ccatcatttt ctatgtctga accaccaaga tcaggaatga
    18481 tgtaaattgt ttgcgcactg ttttctattt ccatttttaa cattagagca gtatctgtag
    18541 ggtcgttagt atctgagggc agttccacag aaataatcgg cacgactgtt tggccctcaa
    18601 agattgtccc aggttggaca atcttgttaa gcgctgcctc aaccatggcg ataagagctc
    18661 gttcgactct gccaacttga agctctggct tgatgggagt gggatctatg ttgcatgcct
    18721 ctagaaccgc gatggccaca cctcctaagg cgagcatccc agctcctatg agaagccttc
    18781 tcctttcttt gggatcaacg ggttctgtag ttccgtgaac ttgattgagt gcagatacga
    18841 gacgctcttt aagtaagttt tgcatagtat gattgcaatg aatactgaca accgataatt
    18901 atatatcaaa agatatcaaa agtcacatat tttgattaac attatttgct atactgagtt
    18961 tataaaaaga tttgaaagga ggagttatat acgaagaaat ctttgagcgt ggggtcctga
    19021 gtgaatcagg aagacgagga tgagggccac ttgagtaaaa tcaattggaa tcggaccagt
    19081 tatgtacatt agtggagtaa ctgagtatct gcggaaaccc tctgggcgag tgaatgtcca
    19141 caagtgctaa gtcaaaacta ctcggtaacg agtaatcaca aagcttgagc atatttcacc
    19201 caaagtcatt tactttggtt tttctgtaat tatgtaatct aaaaaggagc atatatgtgt
    19261 ggtatttttg cgtattttgg tacaaaagag gcagacagta ttgttcgcca gggcttacaa
    19321 aacttggagt atcgtggtta tgattcttgg ggaattgccg ttactgatgg tagtcaagta
    19381 gggatggtaa aagccattgg tgaggtaccc aaaaaattgt ctgataaaca aaagataaaa
    19441 ggacaaatag gcttgggcca tacacgttgg gcaactaatg gaggagtcac tcaggtaaat
    19501 gctcatccgc acctggctaa atctggggaa ttcgcagtgg tccataacgg tattgtggag
    19561 aatgctgatg agttaaaaag tggattaatt aagaggggat ggcgatttca aagtgaaact
    19621 gatactgaag taatcgttta tttacttgag gaggctttgc agaaacaaca gaatttagtt
    19681 ttagctgtta aggcggtttc agcattgcta cagggaagga atacttttgg tattttgaca
    19741 aaatctggtg agttattggc aatgaaaaaa ggttctccac tggttgttgg tcgaagcaaa
    19801 actctagatg agtattttct ttcttcagat acagcttcat tagttggttt agcagatcag
    19861 tttctagttt tagatgacag acaattagta gtggtacgga aaaatggggt cgagttaccc
    19921 caactaggac tcactaatgg aaaaggggaa actattcagg ctcaatggga aaaactggct
    19981 ttggatgatc aatcttcaaa attaaccgag aagcactata tgattcaaga gattttggaa
    20041 acaccgtcag taatgaagac attggcgcga cagtcaaaag caaagtatca attagtcgcg
    20101 cgattgatta aacaggcaaa aaaagtgttt gttattggct ctggaagtac tggattagca
    20161 gcagcttcta ttgctagtca cctgagagaa tctggagaga ttgaggcaat agcactggtt
    20221 ggtgccgaag c
//
LOCUS       DAOQZP010000015        19326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_3193_length_19326_cov_5.861657, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000015 DAOQZP010000000
VERSION     DAOQZP010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 19326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19326
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3193_length_19326_cov_5.861657"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PKH60_01690"
     CDS             complement(<1..269)
                     /locus_tag="PKH60_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16795.1"
                     /translation="MSASKERLVGTEIQSIIREIQDKRFNFYHFRQIMTQFPGLLHQI
                     TTREEEAPMPGKSLVETFCLVFGNKLINLKSFSFLEPADQRLRSP"
     gene            568..1164
                     /locus_tag="PKH60_01695"
     CDS             568..1164
                     /locus_tag="PKH60_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16796.1"
                     /translation="MPNKELEPTNKPVGSLLASDPRYFEINLLQEGIIDAAQALLAKE
                     SVLTHPSEKIQQFVAGRRTDLPTTPEIELMGYLEGIKARQVALLQMDPNDYRQTPDIQ
                     LLRDIVSGLMYVEGSEVEVPTSHSGTDFQPEETMALVKVGEGSSENVEDGAWPLDRSD
                     AEWDELFQQTAFSDEYAWVESLFATELVYREFFRESKV"
     gene            1208..1384
                     /locus_tag="PKH60_01700"
     CDS             1208..1384
                     /locus_tag="PKH60_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16797.1"
                     /translation="MNDQLSTIPYSARGLRALQEALAIHRDMYVWLNPVTGQIDVKEV
                     NQPLRPTLVSRHDD"
     gene            1644..1844
                     /locus_tag="PKH60_01705"
     CDS             1644..1844
                     /locus_tag="PKH60_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16798.1"
                     /translation="MQQVSNKVASRSELLELVETLGMYAQIEQPSFKQCQPVSSLRRV
                     GRLPQLLPVQLKNSVSFRLANR"
     gene            complement(1870..2718)
                     /locus_tag="PKH60_01710"
     CDS             complement(1870..2718)
                     /locus_tag="PKH60_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16799.1"
                     /translation="MTEIPPIGLPNSRNILNFVFIVVSLMLSLSSLAFYSLWRASEVT
                     QSIDSSRENQSSAADLVGAVLPTSKPPAPSFIYPPSGDEKNNWQTIETSFQTEVGFEM
                     AYPKTLLPIKEDGLRSKENFWNPLLKYHFFINEADKKAWSECSKKYAVWIAGLQNPSS
                     TNGNSNRVPPCTPSSFEIFSVSFDTAETPAVNSSPSSVATSANHRSWSIGNLIENGKN
                     GEYGYEAKTVLNDSGLIIQVFLIWPSKEALNKTESLFGYDSKTLLNNLLTQMNFGEHG
                     MYGKTN"
     gene            2867..3436
                     /locus_tag="PKH60_01715"
     CDS             2867..3436
                     /locus_tag="PKH60_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16800.1"
                     /translation="MSEEKEVKDSSSPELGDSDVSTDENSMSDDESEEENHRLRAALI
                     TIIGYVPLPTLFYYLWNSRIGRNEYSSSEALLALYSDLLTFNLLVLPIILTHINHLPE
                     DTERTAASITMLWVAYLRYALMPIGFSMVNRHSSEIKELFEDALGIVGLILEELEVPN
                     DYYDKVSYGTDLYEMNLSAFSSTVDSSSK"
     gene            3612..5360
                     /gene="thrS"
                     /locus_tag="PKH60_01720"
     CDS             3612..5360
                     /gene="thrS"
                     /locus_tag="PKH60_01720"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNQ16801.1"
                     /translation="MSENNQKEQLAELRHSAAHLLAAAVLGLYPKAKLAIGPSIDEGF
                     YYDIDFGTESLGDEQLESIENKMRELVPNWQEFKREEVSAEQAKRRFAGNPYKLELIT
                     DLEKAGEVITLYSSGEFIDLCRGGHVDQPKEQLKFFKLLSVAGAYWRGSEKNPMLTRI
                     YGTAFFSQAELDEYLRIREEAKLRDHRKLGKELDLFTFSDLVGAGLPLWTPKGTLLRN
                     LLDNYVWQLRQAKGYQKVTIPHITKKELYETSGHWSKFANDLFRIVTREDHEFAMKPM
                     NCPHHTQIYNHLPRSYRDVPQRYAETTMVYRDEQTGELNGLSRVRCITQDDAHVFCRE
                     NQLKEEMFAIWDIIEQFYLACGFPKLNLRLSLSDPNQMEKYLGSREQWDMAEEQLRSF
                     AKERGAEFVEAVGEAAFYGPKLDFITKDSLGREWQVATIQADRNMPERFDLFCINEAG
                     EKERVVMIHAAIMGSIERFVSILIEHHAGKFPFWLAPVQVKILPITDGQIEYAQQVMQ
                     QLMEAGLRVEIDERSERLNAKIRDAQLQKVPYMFVVGGKEAESQSVAVRSRDAGDLGV
                     QTIAESIDKLLQETAK"
     gene            complement(5400..7265)
                     /locus_tag="PKH60_01725"
     CDS             complement(5400..7265)
                     /locus_tag="PKH60_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNQ16802.1"
                     /translation="MRPAQQELSRREFLKLAGLATAATMFPFPRLLVEAGVQNPETEQ
                     ACPLTEFSPHYLVQQGGIDGAWVPLPVNAQVEAATQILGKAYIRLKDDNDVVYFLNPL
                     AIEPKYVAQSPLERIERLVREYSLPLENIPNLSNKELLHEVRFKLMANEEHESQGYLF
                     EITSDKQALVWLPQRLSERYFETEGVTIYPQLICVPDSLIVGFDPDTELTNNIEPTKK
                     SATRNTPEKSLPDLALEQLWQQDYPKTHEAAQSFDWQNKVGYQHNPEMLRGESIAETA
                     ENLLKYCTSSGGGGTFFIQTLRSLYPQFEPSIQTISLRNSFTVNGVAYITGINQSNQD
                     GIYKIANNTVERIISFTAENAQGRRVGGAFPLPEKADRFWVVSFYQSSGIQDSLELID
                     LHNQPNGNRKLRFPSIGNNPYYDLYCIYVKFNPTGTHAMVTRYGFGFQPPPPTGRVDE
                     GGGEWIVDLAELTRRALIPGTGDIEVPTVRITGTNHSFGLGFTDGYFDKYVQTATTPS
                     TVQNRVYLPLILNRFLANLSIRGSEALCWHKNNDVDTYRLPSAQPTGLVPRTNLGLTT
                     GWNPHERISGTIGSEARSLAYTYPRPNMGGPHTAIFGVLPMRKNSAGSFEFVEIP"
     gene            complement(7381..8103)
                     /locus_tag="PKH60_01730"
     CDS             complement(7381..8103)
                     /locus_tag="PKH60_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16803.1"
                     /translation="MGSKRNVSMSATTDTVKKVVVDEPVVEETDGAAKTSEKKSTKVA
                     KSRRGKRYVAARSIIDKTKPYTIAEAVELVKKTSYGKFDSSITVDATVKEIGDQGSVS
                     FPHSTGKTLRIAIASDEVLFELESGNANFDVLLTTPAFMPKLAKYARLLGPKGLMPNP
                     KNNTIVQDPEARKKELEKGKVSIKSERKAPLVHMIVGKTSFDSKQLVENIEALVTALD
                     YRLKKLSISAAMGPGVKVKLTK"
     gene            complement(8118..8543)
                     /gene="rplK"
                     /locus_tag="PKH60_01735"
     CDS             complement(8118..8543)
                     /gene="rplK"
                     /locus_tag="PKH60_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002598913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNQ16804.1"
                     /translation="MAKKILQVLKLNLPAGSATPAPPVGPILGQAGINMMDFIKQYNE
                     KTADKRGQIIPAEITVYQDRSFSFVLKLPPVSDMIKQSLKLGKGSAKTGLEPVGKMTM
                     AQVRDIAEKKLGDLNTTDVEQAAKTVAGTARSMGVTITE"
     gene            complement(8580..9224)
                     /gene="nusG"
                     /locus_tag="PKH60_01740"
     CDS             complement(8580..9224)
                     /gene="nusG"
                     /locus_tag="PKH60_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNQ16805.1"
                     /translation="MSNKKATLPKVDQEISTPEVKESAAVAANLVIISTQENKRARWY
                     VVHTYSGHENKVAEMIKQRAETLSLTDKILEILIPSQDKIQIKRGQRKTVNEKIFPGY
                     MLVHMELSDDSWLAVRTTQGVTGFVGIGSKPTALPKHEVEAIRKYMGQATPQYKADYS
                     EGEAVQIVDGPFTDFLATVSNIDEAKGKVEVLVNIFGRETPVALDFLQVKKVGT"
     gene            complement(9241..9426)
                     /gene="secE"
                     /locus_tag="PKH60_01745"
     CDS             complement(9241..9426)
                     /gene="secE"
                     /locus_tag="PKH60_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNQ16806.1"
                     /translation="MVSPTQYFREVIQELKKVSWPSRIQTQEKTLVVLATSLVIGLYL
                     GLADYIFQQIVALAINK"
     gene            complement(9489..11342)
                     /locus_tag="PKH60_01750"
     CDS             complement(9489..11342)
                     /locus_tag="PKH60_01750"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16807.1"
                     /translation="MKVKFSSSTSLKHRVLRPANFLTIIITSLAMVLGFTFLLIIVFI
                     KPYEETQDLRQQAMEMQGCLTIIDLPRGECEALGTLYESTNGAGWTLNSGEVAWFTTP
                     RACEWVGIACSGNPYLERPEPLEKNVVGINLPSRRMNGPLPPVWEIFDQLTQIDLSNN
                     NLTGSIENSGLLTLKNLVSINLSNNKLDGYLADVFSGDHLLVSLDISRNAGVATDPMP
                     LKPLPTSLAKVRTLKQLILNNTLLGEPIPTAFSELSLNIFLTANTNLCLPDGLKAWYE
                     AISKTDNLRFCSATVPTLSPTPTKTPTPSPTGRPSATPTPTATSGTGGTTIVGCNQSC
                     ESNANCSTNLRCYDVGGNKRCRLATNPANESCQGIPDQGLNFACNEYCSDSSECAGDL
                     TCWYNRCREAANVSSVSCTPPDADLNQLMQDNCGDSCSSNRDCVINMRCYSGSCRLAT
                     NPTSQSCSVAPPVKRTTPTVSQPKGGLGNPSITPALSSSPKVSPTATEASESAVLEPS
                     PTAIIPITQTPPEPENKSGLFDQIRNWFSQLKFPEFDASGVSNVIQSKGLLIGLVIAG
                     IVFLLLGIFTGKKKNTPKASSLQAPPTIRSSQESSSGMIDRIKQKGITPLK"
     gene            complement(11332..12291)
                     /locus_tag="PKH60_01755"
     CDS             complement(11332..12291)
                     /locus_tag="PKH60_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16808.1"
                     /translation="MPDSLTTSLRSPKAIIITLGGILIMSIGVIIALVVSSSSVDPRK
                     SASVPGGTVEVSLSPTARTLNPGESTTVTVTLDTKGTPISGIAVRVLYPFSGTVPAMT
                     ASNPNPLISQRDSNWSCQVVSTTVDTNVVKIDLGCLYVAQAGFISSTPIPLESFTLTA
                     GSVGVSTPYTLSFEPASTVVTSKATGEDIAAIPTNTSIITVSATTAATPTPTPTPTIG
                     VTATPTPTSIPGLAVCNQTCIATRDCESGLSCLSGRCRTERCANDTTCLCTDYNVASQ
                     SGTTELPDSGGVTYTMLLILIGSLLTISGGSYFLYAFNKPYES"
     gene            complement(12300..12863)
                     /locus_tag="PKH60_01760"
     CDS             complement(12300..12863)
                     /locus_tag="PKH60_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16809.1"
                     /translation="MNQLKKIISICVILGSSLLLTSCTLLQPKPPSQSPTDTTEGNQP
                     LSATIVLESSNEASFSAELQFIPTETMPLSGLALRLIFPKQTIEDGVDPFIIPETTRG
                     KFWQPLINQVDCVSDADSCYADLALLTLNPSGSDFSGLNQIAGLNPTVFSATMIESLQ
                     MDATNSKATTKKADVIVLELINNVQSN"
     gene            complement(12867..14708)
                     /locus_tag="PKH60_01765"
     CDS             complement(12867..14708)
                     /locus_tag="PKH60_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16810.1"
                     /translation="MHQKHAWRAVLSVAADRVKSQVSALLLVGGVFALVIGTIGLIVG
                     LGLLSQQQDLRQQASSPTGTVEISLRPPTNPTSVNQTVPIIVELNTKSMQVDGVQLAV
                     VLPKSFTSPTARINSTLPLQIVFQQLEESATTNILKLIVINSQISQPFATNTLQPILQ
                     IDSTPNQGGAFPVEADNSRSIVTQHDSDPPRDVLKPITSVSVTITGGSQITATPSPTI
                     AAGGTPAPTPPCTTFTRPEQTSVSCLANQGLSISWTDTNPNPLHPAEEAGFKIYRIDN
                     DPVYTQTGTPGNPPVATLPSHPGSGPMNYVFPDPEVRFGYIPGKFYRFLIYTWKEPLP
                     GRPVGCWKQSAIGTTCSVSTIPTATPTLGPTATLTPTPTVPTTTPQPSVVPGCNALCR
                     SDLECQSNYICYFGPNNSMDSGRCRLKTNPESLTCQPPVPIETPRLAFAAKLQGLSKP
                     STSVKAQVWLEPLNHLDTTADIVQTGYTFDLEMKTGSDGYLYPANQLILTNVPSNQKY
                     VVYLKTTWALRRKLGEIFIASGENKAPLDWVKTPLLVGDFITTPPIQSNKINLLDLSA
                     MLRIYTRLTSPVTATNQQFDVNYDGRIDILDLSLVLSNYTKLEVVGD"
     gene            14777..15658
                     /locus_tag="PKH60_01770"
     CDS             14777..15658
                     /locus_tag="PKH60_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNQ16811.1"
                     /translation="MTEKKVKKAIITAAGFGSRFLPITKTFPKEMLPIIDKPIIQYLV
                     EECAEAEIEEVIIIASPGEVTKFEDYFYGSATNIRTLMIRQGKLDRWHKVEQVFKLPK
                     ITIMPQDERMPYGNGRPVLTARDLVEHDEAFVVMWGDDMVLGEVSAVKQIVDYYNANP
                     CDGVLAVQEVEDSEVDRYGIVKLKPGSDVQIETVIEKPKKSQAPSNLASYGRYVLTPK
                     IFEYLKVDATGLDDELWLQDANDQMSKHGDVRVKVVDGEWMTTGDPIRYMKAHIKYML
                     AHESFGPELKKWLKTLG"
     gene            15925..15996
                     /locus_tag="PKH60_01775"
     tRNA            15925..15996
                     /locus_tag="PKH60_01775"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15957..15959,aa:Thr,seq:ggt)
     gene            16001..16083
                     /locus_tag="PKH60_01780"
     tRNA            16001..16083
                     /locus_tag="PKH60_01780"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16035..16037,aa:Tyr,seq:gta)
     gene            complement(16049..16465)
                     /locus_tag="PKH60_01785"
     CDS             complement(16049..16465)
                     /locus_tag="PKH60_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNQ16812.1"
                     /translation="MKKVLLDTNAYSALMVGNGLILQTLESAHQVVMSPICLGELFAG
                     FKLGTKEKTNKAILSEFLKQPTVELTPITAETAEWFARVFTDLKQNGTPIPLNDVWIA
                     SQAMEHGAQLLSLDKHFQVVPGLRVVPGVGFEPTKT"
     gene            complement(16476..16736)
                     /locus_tag="PKH60_01790"
     CDS             complement(16476..16736)
                     /locus_tag="PKH60_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16813.1"
                     /translation="MNHSSVSLTIHNLDPVLYRVLKQKADTTDVSLNRLIKTLLTQSL
                     GLNKTTKVSDFSEFSKQWTREELKQFQQTQAQFSTVDATDWQ"
     gene            16843..16915
                     /locus_tag="PKH60_01795"
     tRNA            16843..16915
                     /locus_tag="PKH60_01795"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16876..16878,aa:Thr,seq:tgt)
     gene            16960..17160
                     /gene="rpmG"
                     /locus_tag="PKH60_01800"
     CDS             16960..17160
                     /gene="rpmG"
                     /locus_tag="PKH60_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNQ16814.1"
                     /translation="MAKKKKGPRQSVGLKCDACGAFGYITEYNKNNEQLKKQSGDGTF
                     PLNKYCKVCKKHTVHKQSKKLK"
     gene            complement(17162..18493)
                     /locus_tag="PKH60_01805"
     CDS             complement(17162..18493)
                     /locus_tag="PKH60_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16815.1"
                     /translation="MQHLLDPNLVKVFKKDKQWLEEAEIPIVTVAGTMREDIKGWHGL
                     IEDETITDVVLSRAHFSMALAIAFQVWGGQLDHQKAWLVDPTNYVSQEKWQSITFTET
                     VGKILARNPILKTAKDLIDRFGRQQLPILDSITPPLLYLTEHITKPILSLHVAAGNIL
                     LEHGKTVLQVITDPHVRYDYLTQIDNPKLAFCVFDQLTKDDLLEKVALQGKSIDPHRV
                     VVTGPPIDQRVFAARKNKRPWRSGRLNLCITTGGLGTNKSEILELLEQLLPILRSNER
                     NINLLVYCGTQVDLYTNCLDLARQNAIVVGSPADDTAAFRCLYHPQIVDANEQLISFA
                     FPWADGFITKPSGDMAYDAVASGSFLLTLNEWGEWESVIRQRFENQGIARPVITDNFI
                     TQLNELMSAKGQNSSWIERAMLAARKIPPTSINGTDAIINAYHQLAKQKKF"
     gene            18518..>19326
                     /locus_tag="PKH60_01810"
     CDS             18518..>19326
                     /locus_tag="PKH60_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16816.1"
                     /translation="MSEIYSVFNLIWLVKAVLFLIASVVSFYLPGIVLSHWAGITDQK
                     KHLLAWMIGIGFWGIQGYAFGWLQVRWLSFMYLAIFVWQAWRIRKNWWPITYHLGSTS
                     LQKVILVIGTILLLIPVIGSGWRTKEGIAYYWINAYDGLYHLSLSRSLVESVPPIEPG
                     AYDLVVQNYHYLSNLIIADFSRVWQLPINHLYFQFFPVLIAVLLGLTVLKLLKTWSSH
                     KTTVTLGLLLFYLAGEISWLLTWILKSGTELPFNNYIDSGVIQFLNPPQAFA"
BASE COUNT         5299 a         3759 c         4495 g         5773 t
ORIGIN      
        1 tcaggtgacc tcagtctctg atcggctggt tctaaaaaag aaaaactttt taagttaatc
       61 agtttattcc caaatactaa gcaaaaagtt tctacaagac ttttcccagg catcggagcc
      121 tcttcttccc tcgttgtaat ttgatgtagt aaacctggaa actgagtcat tatttgtcgg
      181 aaatgataga aattaaagcg tttatcttga atttctcgaa ttattgattg aatttcggtg
      241 ccaactaatc gttctttact agcactcata gtttactcct ctcttaggtt tgagcatttg
      301 ctatagtatg atcactaata taccacaagt aaatctacac actattgacg tatttaaaac
      361 tatgttatac tggtacacga tgttaaatca ccgtatgtat tctagcttcc aatgtattat
      421 tacccttaag caaggaggtt gtgtcttttt gtaacaaata aaaacccgag caagcaaaaa
      481 aacacaaccc ccgcaaaaaa agcgggggtt tttcgtaggt aaatcaaaac gtagtagcaa
      541 agtagtaaac aacaaaagaa agcaacaatg ccaaacaaag agcttgaacc aaccaacaaa
      601 ccagttggat cattgctagc atctgaccca agatactttg aaattaacct tctccaagag
      661 ggaataattg atgcggcaca agctttgctt gccaaagaat ctgtgcttac ccatccatca
      721 gaaaagatac aacaatttgt ggcaggcaga agaaccgatt tgccaacaac gcccgaaata
      781 gaactgatgg gatatttaga aggtattaaa gcgcgtcagg ttgcactgtt gcaaatggat
      841 ccgaatgatt acagacaaac tccagacatt cagctattac gagatatcgt ttctggatta
      901 atgtacgttg agggcagtga agtggaagta cctacatctc attctggtac tgactttcag
      961 cccgaggaga caatggcgct cgtcaaggtt ggggagggga gtagcgaaaa tgttgaagat
     1021 ggagcttggc cgcttgatcg ctcggatgcc gaatgggatg aattatttca acaaactgcg
     1081 ttttctgatg agtatgcttg ggtggagtct ctttttgcca cagagctggt ctatagagag
     1141 ttttttagag aaagtaaagt atgaacaaat ccgtattact aacagttaca aatgaaagga
     1201 ggacgtgatg aacgaccaac tctcaactat cccatactca gctcggggac tcagagccct
     1261 acaagaagca ctcgctattc accgtgatat gtatgtctgg ctcaacccag ttactggtca
     1321 aattgatgta aaggaagtca accaaccact tagaccaacc cttgtatcaa gacatgacga
     1381 ttagtcgtat gtcgtggagt gacggcgctt atggaggggc gccagttcgc tccgcctgct
     1441 tccttgggcc attagtggcc agggagcaag cagagcgaat tacttagata gatattataa
     1501 ttttattatt agatcgcatt tactcactac gcaagtgtgc ttcattgtct gttttatttt
     1561 gtcagtatat agtgcactta agcctgtccg agcagcgtgg gacgggtttt tttactacta
     1621 tacgcttgca gaaaggaaaa attgtgcaac aagtttcaaa caaggttgcc agtcggtcag
     1681 agttgcttga gttagtagag acccttggta tgtatgctca aattgagcaa ccaagtttca
     1741 agcaatgcca accagtgagt tcgctaagga gagttggtcg tttgccgcaa ctactcccag
     1801 tgcaattgaa aaattcggtt agttttcgat tagcaaatcg ataagagcgg gaaacggttt
     1861 aattgggcac tagttagttt ttccgtacat tccatgttct ccaaagttca tttgtgtaag
     1921 caagttattt aagagtgttt ttgagtcgta accaaaaagc gattctgttt tattcagtgc
     1981 ttctttgctt ggccaaatta gaaaaacttg aataatcaaa cctgagtcat ttaaaacagt
     2041 tttcgcctca tatccatatt ccccgttttt cccattttca attaaattgc caatagacca
     2101 cgatcggtgg ttagcgctgg ttgcaactga agaaggagaa ctatttaccg caggtgtttc
     2161 tgcagtatca aaagatacgc taaaaatctc aaaagaggat ggagtgcacg gcggaactct
     2221 gtttgaattg ccattggtgg atgaaggatt ttgtagccca gcaatccaaa ccgcatactt
     2281 tttactacat tcagaccaag cttttttatc tgcttcattg ataaagaagt ggtatttcaa
     2341 cagaggattc caaaaatttt ctttacttcg caagccatct tcttttatcg gtagcagcgt
     2401 cttgggatac gccatctcaa agccaacttc tgtttgaaaa ctcgtttcaa tggtttgcca
     2461 gttatttttt tcatccccac ttggtggata gatgaaacta ggagcggggg gcttggatgt
     2521 aggcaaaacg gcgccaacta gatcggcagc agatgactga ttttcacgag aggaatcaat
     2581 cgattgggtt acttctgaag ctcgccaaag agaataaaag gctaaagagc tcaggcttaa
     2641 cataagactt acaacaataa aaacaaaatt gagtatgttt ctactatttg gtaaaccaat
     2701 cggtggaatt tcagtcattc tatcagtgta gcaaattttt ttctgatgtc cggtgaggaa
     2761 aagcgtgagc gatcttttga tcgcaaatga atttgggaag acggtgtcag cagttgaata
     2821 tatcaaatac tgtcatataa tatccatata attaatatgg agttatatgt cagaagaaaa
     2881 agaagtaaaa gatagttcga gtcctgagtt aggggattca gatgtttcaa ctgatgagaa
     2941 ttcaatgtcg gatgacgagt cagaggagga gaaccataga cttcgcgcag cactgattac
     3001 aatcattggt tatgtgccct taccgacatt attttattat ctatggaatt caagaattgg
     3061 cagaaatgaa tactcttcca gcgaagcgtt gttagcattg tattctgatc tgctaacatt
     3121 taatttgctt gtattaccaa ttattctcac ccatattaat catcttccgg aagatactga
     3181 acgaactgca gcatcgatta caatgttatg ggttgcatat ttacgatatg cactgatgcc
     3241 aatcggattc tcaatggtga atcgtcatag ttctgaaata aaagagttat ttgaagatgc
     3301 gcttggaata gtaggtctga tattagaaga attagaagtt ccaaatgatt attacgataa
     3361 agtctcttat ggtactgatt tgtatgagat gaatttgtct gcattttctt caacagtgga
     3421 ttcaagtagt aagtaaatac atgttatact aaacgctgac tctatgaaat tttgttatcg
     3481 attcactact acaactacta tccctgcttg agggtggtgt tgtggtaaaa agtccataag
     3541 cccccttgag acagggggtt ttttcgtaga aaacactata taatcaaatt aattgcattt
     3601 gaaaggaaac tatgtcagaa aataatcaaa aagaacagtt agcagagttg cgccattcgg
     3661 cagctcattt gttggcggcg gcggtcttag ggttgtatcc caaggccaag ctggcgattg
     3721 gcccgagtat cgacgagggc ttttattacg atatagattt tggcacagag agtcttggtg
     3781 acgaacaact agagagtatc gaaaataaaa tgcgtgagtt agtgccaaac tggcaagaat
     3841 tcaagcgaga agaagtgagt gcagaacaag caaaacggcg atttgccggt aacccataca
     3901 agttggaatt aattactgat ttagaaaaag cgggtgaagt gattacgctt tatagctctg
     3961 gtgaatttat tgatctttgc cgcggcggtc atgttgatca accaaaggag caactaaagt
     4021 ttttcaaatt attgtcagtg gctggtgcct attggcgtgg gagcgaaaaa aatcccatgc
     4081 tgacaagaat ttatggaacg gcattttttt cgcaggccga attagatgaa tatttgcgca
     4141 ttagagaaga agctaagttg cgtgatcatc gcaagcttgg taaggagcta gatctgttta
     4201 ctttctctga tttggttgga gcgggcctgc cgctctggac acccaagggt acattattac
     4261 gtaatttatt agataactac gtctggcagt tgcggcaagc caagggatac caaaaagtga
     4321 cgattcctca catcactaaa aaagaactct atgaaaccag tggccattgg agtaaatttg
     4381 ccaatgattt gttcagaatc gtcacccgcg aagatcatga atttgccatg aagccgatga
     4441 actgtccgca tcacacgcaa atttacaacc acttgcctcg ttcatatcgg gacgttcctc
     4501 agcgctatgc ggaaaccacc atggtctacc gagatgaaca aacgggagaa ctcaatggtc
     4561 tctcgcgtgt tcgctgtatt acgcaggatg atgcacacgt tttttgccga gaaaatcagc
     4621 tcaaagaaga aatgtttgct atttgggata tcattgagca gttttactta gcttgtggtt
     4681 tccccaaact caatttgaga ttgagtttgt ccgatcccaa tcagatggaa aagtatcttg
     4741 gatcgagaga acagtgggat atggccgaag aacaattacg ctcttttgcc aaagagcgag
     4801 gcgctgagtt tgtagaagca gtaggcgaag cggcttttta cggtcctaag cttgatttca
     4861 ttaccaaaga ttcactcggc cgcgaatggc aagtggccac gattcaagcc gatcgtaaca
     4921 tgccagagcg ttttgatttg ttctgtatta acgaagcagg ggagaaggag cgcgtggtga
     4981 tgattcacgc agccatcatg ggttccatcg aacggtttgt ttcgattctc atcgagcacc
     5041 acgcgggcaa gtttcctttc tggttagctc cagtgcaagt gaaaattttg ccaattaccg
     5101 atggccaaat tgagtacgct cagcaagtta tgcaacaatt aatggaggct ggcttgcgtg
     5161 ttgaaatcga tgagcgatcc gaaagactca atgccaaaat tcgcgatgcg cagctccaga
     5221 aagtgcccta catgtttgtt gttggtggca aagaagccga atcccaatcc gtggccgtcc
     5281 gcagtcgtga cgctggagac ttgggagtac agaccattgc agaaagtatt gataaattat
     5341 tgcaggaaac agcaaaataa agacagtgcc gcgcgatgta tttaattata gtagctgaac
     5401 tatggaatct ctacaaattc aaatgatcca gcactatttt tccgcatagg caggacccca
     5461 aagattgctg tgtgtggacc acccatattt ggccgagggt aggtatacgc cagcgatcta
     5521 gcctcactgc caattgtacc tgatatcctt tcatgaggat tccatcccgt ggtaagacca
     5581 aggttggttc ttggtactaa tccagtgggt tgtgcggatg gtagtctata cgtgtcaacg
     5641 tcattatttt tatgccaaca aagcgcttct gaacccctaa tagatagatt tgccaagaat
     5701 ctattcagaa tgagtggcag gtagactcta ttttgaacgg tactcggagt tgtggcagtc
     5761 tggacatatt tatcgaaata tccatcggta aaaccgaggc cgaatgagtg attggtgccg
     5821 gtaattcgaa cagtgggaac ctcaatatcg ccagttccag gtattagcgc tctccgagtg
     5881 agttctgcaa gatcgactat ccattctcca cctccttcgt cgactctgcc ggttggcggt
     5941 gggggttgaa aaccgaagcc ataccgggtt accattgcat gagtgccggt gggattgaac
     6001 tttacataaa tacagtacaa atcatagtag gggttattgc caatagaagg gaatcggagt
     6061 tttctgtttc catttggttg attgtgaaga tcgatcagtt cgagagaatc ttgtatccca
     6121 cttgattgat aaaaactcac cacccaaaac cgatcggctt tttctggtaa tggaaacgct
     6181 ccacccactc ttcttccttg ggcattttca gcagtaaaag aaatgattcg ttcgactgta
     6241 ttattcgcaa ttttatagat gccatcttga ttgctttgat ttataccagt aatatatgcc
     6301 acgccattga cagtaaatga atttctcaaa ctaatggttt ggatgcttgg ttcaaattgt
     6361 gggtagagcg atctcaaagt ttggataaaa aaagttccgc ctccaccact actcgtacag
     6421 tatttcagca aattctcagc cgtttctgcg atggattcac cgcgaagcat ttctgggttg
     6481 tgttgatagc ccactttgtt ttgccaatca aatgattgtg ctgcctcatg cgtttttggg
     6541 tagtcttgtt gccaaagttg ctcaagagct aagtcgggga gcgacttttc tggcgtattg
     6601 cgagtagcgc ttttcttcgt tggctcgatg ttgtttgtca gttcagtgtc agggtcgaaa
     6661 ccaacgatca gagaatctgg tacgcaaatt agctggggat agattgttac accttctgtt
     6721 tcaaaatatc gctcacttaa tcgttgggga agccagacaa gcgcttgctt atcggaggtt
     6781 atttcaaata aatatccttg tgactcatgt tcttcatttg ccatgagctt gaatctcact
     6841 tcatgaagca gttctttatt gctcaaattg ggaatatttt caagtggtaa tgagtattct
     6901 ctaacaagtc gctctattct ctctaaaggt gattgtgcca catattttgg ttcaatggcc
     6961 aacggattga gaaaatagac aacatcgttg tcatccttca agcgaatata tgccttgcct
     7021 aagatttgag ttgcggcttc tacttgcgcg ttgacaggca gtggcaccca ggcgccgtcg
     7081 ataccacctt gttgcactaa gtaatggggt gagaattcgg tgaggggaca agcttgttct
     7141 gtttctgggt tttgcacacc agcctcgact aagaggcgtg gaaaaggaaa catagtagcc
     7201 gctgtcgcca aacctgctaa tttcagaaat tctcttctac ttagttcttg ttgagctggt
     7261 ctcatacctc tgaattatac cgaataataa acgaaacacc ttactagaac atagaaagat
     7321 gagtacggca tgaactacgg tgatttggaa cactatctac ctaagaaatg ctttgaaacc
     7381 ttattttgtg agcttgacct taactccagg ccccattgcg gcagaaatag agagtttttt
     7441 caatcgataa tcaagtgcgg ttacgagggc ttcaatgttt tcgacgagct gtttgctgtc
     7501 aaatgatgtt ttgcccacga tcatgtgcac caatggtgct ttacgctctg atttgatgga
     7561 aactttgcct ttttccagct cttttttgcg agcttcggga tcctgtacga tcgtgttgtt
     7621 ctttgggttt ggcatcaagc ccttgggtcc tagcaaccga gcgtactttg ccaatttagg
     7681 cataaaggcc ggagtggtga gtaatacatc aaagttggca ttgccggatt ctagctcaaa
     7741 cagtacttca tctgaggcaa ttgctatgcg taaagtttta cctgtgctgt gagggaaaga
     7801 aacactccct tgatcaccga tttctttaac agtcgcatca acagtaatac tggaatcaaa
     7861 cttgccgtat gaggttttct tgaccaattc aacggcttcg gcgatggtgt atggcttagt
     7921 tttatcaatg atggagcgcg cggccacata gcgtttgcca cggcgagact tagccacttt
     7981 tgttgatttc ttttctgaag ttttggccgc gccgtctgtt tcttctacca ctggttcgtc
     8041 aaccacaacc ttttttactg tgtcggttgt tgcactcatc gatacatttc tttttgatcc
     8101 cataactctc ctttaactta ttcagtgatt gtcacaccca tactacgagc agtgccagcc
     8161 acggttttgg ctgcttgttc aacatcggtt gtattcaaat cgccaagctt tttctctgca
     8221 atgtcgcgca cttgggccat ggtcatttta ccaacgggct caagaccggt cttcgcacta
     8281 cctttcccaa gtttgagtga ttgtttaatc atgtctgaaa ccgggggaag tttgagtacg
     8341 aagctaaacg aacggtcttg atagacggta atttctgctg gaataatttg accacgctta
     8401 tcagcggtct tttcattata ttgtttgatg aaatccatca tgttaatacc agcctgaccc
     8461 aaaattggtc caactggagg agctggtgta gcactgccag cggggaggtt caacttaagt
     8521 acttgtaaga ttttttttgc catatgattc cttcaatgaa gtaatttcag tttgttggat
     8581 taggtgccaa ccttttttac ttgtaagaag tcgagagcca ctggagtctc tcggccaaag
     8641 atattcacca acacctcaac tttacccttt gcttcatcaa tattgctaac tgtggccaaa
     8701 aagtcggtaa agggaccatc tacaatttgg acggcttcac cctctgagta atctgcctta
     8761 tattgtggag tagcctgacc catatattta cgaatcgctt ctacttcgtg ttttggcaat
     8821 gcagtgggtt tgctaccaat gcccacaaag ccagttacac cttgggtagt gcgaacagcg
     8881 agccacgaat cgtcgctcag ttccatgtga acaagcatat agccagggaa gattttttcg
     8941 ttgacagttt tgcgttgacc acgcttaatt tgaattttat cttgactagg aatgagtatt
     9001 tcaaggattt tgtcagtgag gctcagggtt tcagcgcgct gcttaatcat ttcagcaact
     9061 ttgttttcat ggccagagta ggtgtggacc acgtaccagc gcgctctttt attttcttgg
     9121 gttgaaataa ttaccaaatt agcagctact gcagcacttt ctttcacttc tggagtagaa
     9181 atttcttgat cgacttttgg tagtgttgct tttttgtttg acatgttgat gactttacta
     9241 ctatttgttg attgccaatg ctacgatttg ctgaaagata taatcagcta aacccaagta
     9301 taaaccaatc actaaactgg tggccaatac cactaaggtt ttttcttggg tctgaatacg
     9361 gcttggccaa ctcacctttt tgagttcttg aatgacctcg cggaagtatt gggtggggct
     9421 aaccataatc aggcgtcata ttgtatgcta taacatcgaa aactgcaatg gactcttagt
     9481 tgtggttctt atttaagcgg agtgatgcct ttttgtttga tgcgatcaat cattccactc
     9541 gagctttctt ggctggaacg gattgttggg ggtgcttgta agcttgaggc ttttggcgta
     9601 tttttcttct tgccagtgaa aataccgagc aacaaaaata caattccagc gatcactaag
     9661 ccgattaata aaccttttga ttggatgaca ttggaaacac ccgacgcatc aaattctgga
     9721 aattttagct gtgagaacca gttgcgaatt tgatcgaata gcccagattt attttctggc
     9781 tctggtggtg tttgggtgat gggaatgatt gcggtaggag atggttctaa tactgcagat
     9841 tctgatgctt cagtggcagt gggacttacc ttgggtgagg aggagagcgc aggtgtgatt
     9901 gatgggttgc ctaagcctcc tttcggttga gaaactgtag gggttgtacg cttaacaggt
     9961 ggggcaacgg aacatgattg gctagttggg ttagttgcga ggcgacagct accgctataa
    10021 cagcgcatat taatgacaca gtctcgattg ctcgagcaac tatcaccaca gttatcttgc
    10081 atcagttggt ttaaatctgc atcgggtggg gtacaactca cgctggacac attagctgct
    10141 tcacggcagc ggttatacca acaggttaaa tctcctgcac actcactact atcactacag
    10201 tattcattgc aagcaaaatt caacccttga tctggtatgc cttgacagct ttcatttgca
    10261 ggattagttg ctaatctaca acgtttattt ccgccgacat cgtaacaacg caaatttgtg
    10321 ctgcagttag cgttgctctc acaactttgg ttgcaaccaa caatggtggt acctccggtg
    10381 ccactcgttg cagttggtgt tggtgtggca gatggtctcc cagttggcga tggtgtggga
    10441 gttttagtgg gtgttggtga aagtgtagga actgttgctg agcaaaaacg cagattatca
    10501 gtttttgaga tagcttcgta ccaagctttt agaccatctg gtaaacatag attggtgttg
    10561 gcagttagaa aaatattgag cgataattct gagaatgcgg tggggattgg ttctccgagc
    10621 aaagtgttgt taagtatcag ttgcttcaaa gtacgtacct tggcaagtga ggtgggtagt
    10681 ggcttgagtg gcattgggtc ggtggctact ccagcatttc ggctaatgtc tagtgagact
    10741 agtaggtgat cgccagaaaa aacatcggcg agatagccat ccaacttatt gttgctcaga
    10801 ttaattgaaa ctaggttttt taaagttagt aatccagaat tttcaatcga cccagtgaga
    10861 ttattattac tgagatcgat ttgagtaagt tggtcaaaaa tttcccaaac tggaggcagc
    10921 ggaccattca ttcgacgact agggaggtta ataccaacga cgtttttttc tagaggctcc
    10981 ggtcgttcta gatatggatt gccggaacag gcaatcccaa cccactcaca agctcttggt
    11041 gtagtaaacc aagccacttc tccagaatta agagtccagc ctgcaccatt tgtagactcg
    11101 tataaggttc ccaacgcctc acattcccct ctgggtaaat cgataattgt tagacaacct
    11161 tgcatttcca tagcttgttg tctgagatct tgagtttctt cataaggttt aataaaaacg
    11221 ataattaaaa ggaaggtaaa accgagtacc atcgccagac tggtaatgat gattgtcaag
    11281 aagtttgctg gtctaagcac gcgatgtttc agtgatgttg atgatgaaaa tttaactttc
    11341 atatggttta ttgaaggcat ataggaagta gctgccaccc gaaattgtaa gcaaactccc
    11401 aatgagaatt aataacatag tataggtaac accaccggag tcgggtaact cagtggtacc
    11461 actttgacta gccacattat agtctgtaca gagacaagtt gtatcattgg cgcatcgttc
    11521 ggttcggcaa cgaccgctta gacaactaag gccggattca caatctcgag ttgcaataca
    11581 agtttgatta caaaccgcaa gtcctggaat ggacgttggt gtgggagtgg cggtaacacc
    11641 aattgtgggc gtgggagtgg gtgttggtgt ggctgcggtg gtggcagaaa ccgtaatgat
    11701 actcgtatta gtcggaattg ctgcaatatc ttcgccggtt gcttttgatg taaccacagt
    11761 gctcgccggt tcaaaagata acgtataggg ggttgaaaca ccgaccgagc ctgctgtcag
    11821 agtaaatgat tctagcggaa taggagtaga gctaataaag cctgcctgcg caacgtacag
    11881 acaacccaag tcaattttga ccacattggt atctacagtg gtagatacaa cttggcaact
    11941 ccagttacta tctcgttggg agatgagagg attgggattt gaggcagtca tagctggtac
    12001 tgtgcctgaa aatgggtaga gcacacgtac cgcaattccg gaaattggcg tgcctttcgt
    12061 atcgagcgtc actgttacag tggttgattc accaggattt aaagttcttg ctgtaggact
    12121 gagtgacacc tcaactgttc cacctggaac agatgcactt tttcttggat cgactgaact
    12181 actagaaacc accaaagcaa taatgactcc aatactcatg atgaggatgc caccaagggt
    12241 aataatgatg gcttttggtg agcgaaggct ggtggtaagt gagtctggca cggtataaat
    12301 cagttacttt gtacgttgtt gattaattca agaactatta cgtcggcttt ttttgttgta
    12361 gccttactat ttgtggcatc catttgcaag ctctcaatca ttgtagcgga aaaaactgtt
    12421 ggattgagac ccgctatttg gttcagtcca gaaaaatcgc ttccgctcgg gttaagcgtg
    12481 agaagagcca gatcagcgta gcaactatca gcatcagaaa cacaatcaac ttggttaatg
    12541 agcggttgcc agaatttccc tcgggtcgtt tcagggataa taaatggatc aacaccatct
    12601 tctatagttt gttttggaaa aatcaacctt agtgctaagc cagacaaagg catagtctca
    12661 gttggaatga attgcaactc ggcactaaaa ctcgcttcgt tggacgattc caacacgata
    12721 gtggcagaca gaggttggtt tccttcggta gtatccgtgg ggctttggct tggaggtttg
    12781 ggttggagca gtgtgcagct ggtcaatagt aacgaagaac cgaggattac acatatagag
    12841 ataatttttt ttagttgatt catagtttaa tctccgacta cctcaagttt tgtgtaatta
    12901 ctcaagacaa gtgagaggtc gagaatatca attcgtccat catagttcac atcaaattgc
    12961 tgattggtag cggttacagg tgaagttagt cgagtataaa ttctcagcat cgcactcaga
    13021 tctagtaaat tgattttatt tgattgtatt ggtggtgtgg taataaaatc tccaacaagt
    13081 agtggtgttt tcacccaatc aagcggagct ttattttcac ccgaagcgat gaaaatttct
    13141 cccaactttc ttctaagtgc ccaggttgtt tttagatata caacatattt ttgattgctt
    13201 ggtacattcg ttaagatcaa ttgatttgca gggtatagat atccatcaga accagttttc
    13261 atttctagat cgaatgtata gcccgtttga actatatctg cagtagtatc cagatgattg
    13321 agtggttcca accaaacttg tgctttaact gaagtacttg gtttactcaa cccctgcaat
    13381 ttagctgcaa atgctaaccg aggtgtttca ataggtacag gtggctgaca ggttaggctt
    13441 tctgggtttg ttttgagtct gcaacgacca ctatccatgc tgttattggg gccaaagtaa
    13501 caaatgtagt tactttggca ttctaaatct gatctgcaca aagcgttaca acctggaact
    13561 accgagggtt gcggtgttgt ggttggaacc gttggtgttg gagtaagtgt ggcggttggt
    13621 ccaagtgttg gtgtggcggt tggaatggtt gatacggagc aagtagtacc aattgcagat
    13681 tgtttccagc aaccaactgg tcttcctggt agcggttctt tccaagtata aattaagaaa
    13741 cggtagaatt ttcctggaat atagccaaaa cgtacctcag gatctggaaa tacatagttc
    13801 attggtccac tacctggatg cgaaggcaat gtggcaacgg ggggatttcc gggagttccg
    13861 gtctgggtat aaacaggatc attgtcaatt ctataaattt tgaatcccgc ttcctcagct
    13921 ggatggagtg ggttgggatt ggtatcagtc caagatatgg aaaggccttg attggccaaa
    13981 caactgacgc tggtttgttc tggtcgggta aatgtggtgc agggaggagt gggagccggg
    14041 gttcctccag cagctatggt aggacttggg gttgcagtaa tctggctgcc accagtgata
    14101 gttactgata ccgaagtaat gggtttcaga acatcccttg gaggatctga gtcgtgctga
    14161 gtgacaattg atcgagagtt atcagcctct acaggaaaag cgcctccctg gtttggtgtg
    14221 gaatctattt gtaaaattgg ttgaagagta ttggtggcaa atggttgtga gatttgcgaa
    14281 ttgattacta ttaatttgag aatgtttgtt gtggcgcttt cttcaagctg ttggaaaacg
    14341 atttgcaaag gtaatgttga attaattctg gcggttggac ttgtaaatga tttgggaagt
    14401 acaacggcaa gctgaacacc atccacttgc atgcttttag tgttgagttc aacgataatg
    14461 ggtactgttt gatttactga ggttggattg gttggaggac gaagtgatat ttccactgta
    14521 ccagtaggac tcgatgcttg ttggcgtaaa tcctgttgtt gagaaagtaa tccgagtcca
    14581 acaattagtc caatggtacc gatcaccaaa gcaaagacac caccaaccaa caagagggca
    14641 gaaacctggc ttttcactcg atcagccgct acagacaaaa cagcgcgcca agcgtgcttt
    14701 tgatgcatca cctattaata gtacactaat caggcgcaca aaggaaagac tattttcatg
    14761 ttgtatactt acacatatga cagaaaaaaa agtcaagaaa gctattatta cagcagcggg
    14821 atttggttcc cgttttttgc caattactaa gacatttccc aaagaaatgt taccaattat
    14881 tgataaaccg attattcaat atttagtgga agaatgcgcc gaagcagaaa ttgaagaagt
    14941 tattattatt gcttctcctg gtgaggtaac aaaatttgaa gactattttt atgggagcgc
    15001 gacaaacatt cgcacactaa tgattcgaca aggaaaactg gataggtggc ataaagtaga
    15061 gcaggtgttt aagttgccca agattacgat tatgcctcaa gacgaacgga tgccctatgg
    15121 caatggtagg ccggttttga ctgcaagaga tttggtagaa cacgatgaag cttttgtggt
    15181 gatgtggggt gatgatatgg ttttgggtga agttagtgct gtgaaacaaa ttgttgatta
    15241 ttacaacgct aacccctgcg atggggtgct tgcagttcaa gaagtcgagg atagcgaggt
    15301 ggatcgatat ggcattgtga aactgaagcc tggcagtgat gttcaaattg agactgtgat
    15361 tgaaaaaccg aaaaagtcgc aggctccttc aaacttagct tcgtatggga gatatgtgct
    15421 taccccgaaa atttttgaat atctcaaagt tgatgctact ggtttagatg atgagctttg
    15481 gttacaagac gcgaatgatc aaatgtccaa gcatggtgat gtgcgggtaa aagtggtaga
    15541 tggtgagtgg atgaccactg gcgatcctat tcgatacatg aaagctcaca tcaagtatat
    15601 gctggctcat gaatctttcg gtccagagct caaaaaatgg ctaaaaactc ttggttaaag
    15661 cttgcagctg tttgttaaga tgtgctacga tcagagtaac tgtgggcact ttaacaaatc
    15721 gcgtgtgaga gacatggcat tttcctggat agattatgtt cgggcttatg gactgtctgt
    15781 gagtcgtaat ggcttatagc ttcctgccca cctcgcttta tgcgggaacg aatgtctctc
    15841 acacgcgggt gctcacttgt ctcgtttttt tctctatctt ttgttggtat aatgccctgt
    15901 cataacttgc tagtcgtgat atgcgccgaa gtagctcagg ggtagagcac catcttggta
    15961 agatgggggt catgggttca attcccatct tcggctcaga gccaggttac ccaagcggtc
    16021 aacgggggca gactgtaaat ctgctgactt acgtcttcgt aggttcgaat cctacacctg
    16081 gcacaactct caaacctggc acaacttgaa agtgtttgtc caggctaagt aattgtgctc
    16141 cgtgttccat tgcttgtgaa gcaatccaga catcgtttaa tggaatagga gtaccatttt
    16201 gttttaaatc agtaaatact ctggcaaacc attcggcggt ttcagcggtg attggtgtca
    16261 gttctactgt aggttgcttg agaaactcag aaagtatcgc cttatttgtt ttttcctttg
    16321 ttcccaactt gaaaccagca aatagttctc ccaaacagat tggggacatg acgacttgat
    16381 gagcgctctc aagagtctgt aagattaagc cgttgcctac catcagggca gaataagcgt
    16441 tagtatcaag taaaactttt ttcatagcac ataccttact gccaatcagt tgcgtcaacc
    16501 gtagaaaatt gtgcctgagt ttgttggaac tgtttgagtt cttctctagt ccactgtttt
    16561 gagaattcag aaaagtctga tactttagtg gttttgttta atcccagtga ctgagtgagc
    16621 agtgttttga ttaagcggtt aagacttacg tcagttgtgt cggctttttg ttttaacacc
    16681 cggtacaaga ctggatcgag attgtgaata gtaagtgaaa ctgatgaatg gttcataatt
    16741 atgttaatta tggttcaaag tacatatttt gtcaagctga tgcttgccac ttcatgccgg
    16801 gtatatttct ggtaaaatgt gggggtcaat tttggaaagc gtgccagagt agctcagtgg
    16861 ttagagcacc tgttttgtaa acagggggtc gtgggttcga atcccacctt tggctcaagt
    16921 atggtacaat gctcggtctc gatcaattaa aggaacacta tggcaaaaaa gaaaaaagga
    16981 ccaagacaat cagttggact caagtgtgat gcttgcggtg catttggcta tatcactgaa
    17041 tacaacaaaa ataacgaaca gttaaagaaa caatctggag atggaacatt ccctctcaac
    17101 aaatactgca aagtgtgtaa aaaacacact gttcacaaac aatccaaaaa attgaagtaa
    17161 attagaactt cttttgtttc gccagttgat ggtaggcatt tatgattgca tcagtgccat
    17221 taatcgatgt gggtggaatt tttcttgcag caagcattgc acgttcaatc cagcttgaat
    17281 tttggccttt tgcactcatt aattcattaa gctgagtaat aaaattgtct gttattactg
    17341 gtctagcaat accctgattt tcaaaacgtt ggcgaataac tgactcccac tcgccccatt
    17401 cattcaatgt taaaagaaaa cttccgcttg caactgcatc ataggccata tctccagaag
    17461 gtttggttat aaaaccatct gcccagggaa atgcgaagct aataagttgc tcattagcat
    17521 caacaatttg gggatgataa agacaacgaa aggcagccgt atcgtcagca gggcttccga
    17581 ctacgatggc attttgacga gcaaggtcta gacaatttgt gtacagatca acctgcgtac
    17641 cacaataaac taaaagattg atgttgcgtt cattacttct taatatgggt aataattgtt
    17701 ctaacagctc taaaatttct gatttgttgg tgcccaggcc gccagtggtg atacaaaggt
    17761 taagcctgcc tgatctccaa gggcgtttgt tttttctggc agcaaaaact cgttggtcta
    17821 ttggtggacc ggtcacgact actcgatgag ggtcaatcga tttgccttgc aaggcaactt
    17881 tttctagcaa atcatcttta gtaagttgat cgaaaacgca aaaagcgagt ttgggattat
    17941 caatttgtgt aaggtagtca tatcgcacat gtgggtcagt aataacctgc agtacggttt
    18001 tcccatgctc cagtaaaata ttgccagctg ccacatggag agagaggatg ggtttggtaa
    18061 tatgctcggt taaatagagt agtggtgggg ttatagaatc taatatagga agttgttggc
    18121 ggccaaagcg gtcaatcaag tccttggctg tttttaggat tggattacga gcaaggattt
    18181 ttccaacagt ttctgtgaaa gtaattgatt gccacttttc ttgactcacg taattagttg
    18241 ggtcaactag ccaggccttc tgatggtcta gctgtccacc ccaaacttga aaggcgattg
    18301 ccaacgccat agaaaaatgt gctctggaga gtaccacgtc tgtaattgtt tcgtcttcga
    18361 taagaccatg ccaacctttg atgtcctcgc gcatggttcc agccaccgtc acgatgggaa
    18421 tttcagcttc ctcaagccac tgcttgtctt ttttgaaaac tttaacgagg tttgggtcga
    18481 gcagatgttg catattggtc tgctatgatt atggcacatg tctgagatct atagtgtatt
    18541 caatttaatt tggttggtca aagcggtgct ttttttgatc gcttcagtcg ttagttttta
    18601 tctgcctggt attgtcttga gccactgggc ggggattacc gatcaaaaaa agcacctttt
    18661 agcctggatg attggcatag gtttttgggg tatccagggg tatgcgtttg gttggttgca
    18721 agtacgatgg ctatcgttca tgtatctagc tatttttgtt tggcaagcat ggagaataag
    18781 aaagaattgg tggccaatta cctaccattt aggatctaca agtttacaaa aagttatact
    18841 agttattgga acgatactat tactcattcc agtaattggt tctggatgga gaaccaaaga
    18901 aggcattgct tactattgga tcaatgccta cgacggcttg taccacctat ctctttcacg
    18961 atctttggtt gaatcagtgc ctccgattga gccaggggcc tatgacctgg ttgtacaaaa
    19021 ttaccactat ttgtccaatc taattattgc tgatttcagt cgagtttggc agttacccat
    19081 caatcattta tattttcaat ttttccctgt tttgatagca gtgcttcttg gtttaaccgt
    19141 tttaaagctg ctcaaaactt ggtcgagtca taagactacc gttactttgg gtttgttgtt
    19201 attttatctt gctggagaga tatcttggtt gctgacttgg attctcaagt ctggcactga
    19261 gcttcctttt aataactata ttgatagtgg agttatccaa tttttaaatc cacctcaggc
    19321 cttcgc
//
LOCUS       DAOQZP010000016        19169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_3238_length_19169_cov_5.621115, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000016 DAOQZP010000000
VERSION     DAOQZP010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 19169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19169
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3238_length_19169_cov_5.621115"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PKH60_01815"
     CDS             <1..746
                     /locus_tag="PKH60_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16817.1"
                     /translation="REILHLAKIGLHPSNFFSPHITIGGLDLSPDHQEPLYIQSLVFG
                     CGFSEFKQADLNLRREKGKFKIKLRERYKKITETPDGIQYVPFLKKRSAEYTKPYGQK
                     QTEQDEDSLRSVEFRLGNRFKSFQNLTREVDAIYYLSTAAIAHQKDASERDPVEVKLS
                     QIWEELAHKLDEIYLQIGIHFPSGATKSFIFGSSDSEEVKAKRGSTPYNTHLFELGYQ
                     SATDAVFRSQIRTLVKNARKEILDCINCR"
     gene            complement(755..>1543)
                     /locus_tag="PKH60_01820"
     CDS             complement(755..>1543)
                     /locus_tag="PKH60_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNQ16818.1"
                     /translation="IQFLFMGVLVIIWGHQPLLTSTNNSKSLSVTQYLFLSGLGILGI
                     VMVGKTLSIRHADVLYTEGKTTIQQGQLAIGSDKILSAIALRPQEASYYSTLGGYYAN
                     IAGQLAAGQQASEAATLASQAATLLENSTQVNPVHYTMYLTKIGSYMQLAPLDLAWLT
                     KALEVVEKAKTLSPTDPRPWLLEGKILGYQSEYEQAIVSLQKALELKPNYAEARGTLA
                     TIYELRNNPQAALEQYHYILKYISPDDTVLKAKVASMEAALNNL"
     assembly_gap    1544..1643
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1644..2788)
                     /locus_tag="PKH60_01825"
     CDS             complement(<1644..2788)
                     /locus_tag="PKH60_01825"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNQ16819.1"
                     /translation="MENSLDRWLKRALVSLFHFGALALPLVFAFNTEELFEFNKMIFV
                     YIWAIITALIWLARMIVQKKVLLVKTPFDLPIIGYIVSQLLATLFSIHPYTSLFGYYS
                     RFHGGLVSTLAYVTLFYAFVATFVGNSQSKKTPFLSQPIAHWLLSLVIGSVLASLYAL
                     PEHFGYSFSCQLATGAFNVSCWVQDVQNRVFGSFGQPNWLAAYLILLIPIGISSLLFM
                     KKRLVWISLLSITTLWYCVVLFTKSRSGLLGFGVSLVFLLGWFALAYFTHSWSIKKVA
                     LKQILIRFSLIGFLLLTSTLYFGTPLTPSITQLLNKSEAPKVETFGPALEAGGTESGE
                     IRKIVWQGAIDVWKRYPFFGSGLETFAYSYYQDRPTTHNLVSEWDFL"
     gene            2849..3445
                     /locus_tag="PKH60_01830"
     CDS             2849..3445
                     /locus_tag="PKH60_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16820.1"
                     /translation="MQDQPVQNPTSNQDPQSYADAYDHPTQSPVANSDHDVVLPPPVS
                     QNTVSASTTSETLEDQNIFYLLGVEDGTNEQKETFLDELQQVIWEDFLENDVQLLLTK
                     EEHQQLQQMTNNDTKKPLMEQEAVVVFLEKLIPDLEEIMLEKALELKADLVKERVAGL
                     KELHAGKTDMLQKIEEAENLVSQDKWLSAARMLNSLPA"
     gene            3473..4474
                     /locus_tag="PKH60_01835"
     CDS             3473..4474
                     /locus_tag="PKH60_01835"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNQ16821.1"
                     /translation="MSLSSIVARIVPSGAFPPLVIDCGSAYTKVAYKGRLIWEQPTVV
                     AIDQQSDRYVSIGTVAVRQLDLDAQRHLRAVYPIQNRMVAHMEGLEWYITQLLNQITW
                     VDGSSISIFTPLLFIVPMGQSRSNSKQWLKFTTTLGRKISFLTEEIVVSKLQTEIFLP
                     TWIIDFGYSHTTSAFYDEDGYVASNSFPWGLNRLIRALERSVTQRNIHLSSEQIEQVL
                     QQLVFVPTIDGSIKKRKIGLSAKQIGNGSSVRLILNQEDVESSVVEIVVEWLWLVKQQ
                     MVQLSQTIERDLTKTKFYCFGGGSLLTGLTQNIERTLSMQMQTNLVAVTSLPPLVTK"
     gene            4476..5249
                     /gene="mreC"
                     /locus_tag="PKH60_01840"
     CDS             4476..5249
                     /gene="mreC"
                     /locus_tag="PKH60_01840"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNQ16822.1"
                     /translation="MFTLNKLFARVAIYLITCIAGLGVAELTGSAQEIQTLVRQNSIP
                     IQIATAQRIQSIKTPISSLFTFWNQLETHRELELAYANLQAQLVHLEQLEQENQELRS
                     QLGTESEKKTQGLITTPVLSYPYRFINYGAQSEVKTGDLVLVNGTLVGRLGEVNLEYS
                     RVILLSDQDASAIIGITNLGQRGVVRGSDQQLVLTEIPQTATITFGDRVMSAGEKGVK
                     PGVFIGTIGKELTKPTDAVKTFVIDQVNTFDTARLVEIQ"
     gene            5256..5720
                     /locus_tag="PKH60_01845"
     CDS             5256..5720
                     /locus_tag="PKH60_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16823.1"
                     /translation="MKLTIQLRLLFLLLLKLSLVYFALLLDFQLGLPMLTCFLLLRFS
                     RGTNQPVWIIIYLMLCSLMFSSMFSLSWLITLSVFGAAFLFDHSMRFNTKQSRFLLLG
                     LSIFISILLFVVAGGMLSGGVVLYGIISLATIIGISQWSWFSRFQSQSWYIR"
     gene            5725..7533
                     /locus_tag="PKH60_01850"
     CDS             5725..7533
                     /locus_tag="PKH60_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNQ16824.1"
                     /translation="MLEQNPNTFFWRARIFLSVLLVSVIVVLARLVDLQLGQVGWYQA
                     AAQDNSDFRLSVPANRGVLLDRYGEALVKNSIRYWQKQDLTTFFSKEVLVDDSSALGL
                     LATQAAQIRKEYSRWYPHNSSGAHVIGYVGPISKDELLLNENAEITDLVGRTGLERAF
                     NTLLTGTAGEIEYEVTALGEANRVIQEKPMIPGAVIKTTLDPYLTAIAQKAMENNKGA
                     VIIADAKTGALLAVVSSPSYDPNVFTKFTQTNEEQALRRQQITSFLEDPDKALFNRAI
                     AGEYPPGSVFKLVTAIAGLESESITPSTVVKDEGILKVGEYAYANWYFSQYGGTEGEI
                     SLERALARSNDIYFYKAAEFTGPDAIATWARNIGFGSPTGIELTGEASGLVPDPEWKE
                     RTLHEQWYLGNTYHYGIGQGDLLVSPIQVSQLFQTIANQGVRCQPHISEDGKNECHSL
                     GMKEENVTPILRGMIAACSTGGTAFPFFAYNEQFEPNPNPNEAIANGMAACKTGTAEF
                     GGADERGFRKTHGWFSVVVGAPQLNNPVVDGELETLNLEDQARNEWLAKIRQNPLPKE
                     LIITVLVESDEEKPYKEGSADAAPIALELLEWMRGK"
     gene            7568..8416
                     /locus_tag="PKH60_01855"
     CDS             7568..8416
                     /locus_tag="PKH60_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16825.1"
                     /translation="MEQDSDQLVLPLIPQEYLQIFPKVVQLIEEFGLLPPTEQTYLEV
                     KKMAEQGLPLVKSLYEDATLHLFKEGKLLSEHDRQGEGFNVIYASILLNKGYGPITLV
                     LSPEIMMEGRFEAYCIDHASTYLSDRVNDYINEHMSSSVVSSAEIQDIENDLMEKYTL
                     NPQEFFVFYSYCVASLCENEEEYFYDDSYEERLRFLTFDIGLPEIKVDDLLAVICKQG
                     WGDQVVDEDNLRDICTQKDKAFLTVGKRDYGSQSLYSQEVAITSKLMEQFQIPMKYRE
                     KMNQVA"
     gene            complement(8525..9052)
                     /locus_tag="PKH60_01860"
     CDS             complement(8525..9052)
                     /locus_tag="PKH60_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16826.1"
                     /translation="MTHPENFPANNLLLTEDPCGYAMALILRAHIVTAGHVASGSPTY
                     HTPHLDQMTDIPLQLIAGRDLAISTVTTVSPGFEKIAPPSLDQCLTIVGFHGFQHVPF
                     EIKVRVIEVQPNGRVIAEWLSGTDAQVGMSGSPAITEQDEVVGILVSGVEGANGKQLV
                     IESLSDLKFDSVTQE"
     gene            9137..10249
                     /gene="dprA"
                     /locus_tag="PKH60_01865"
     CDS             9137..10249
                     /gene="dprA"
                     /locus_tag="PKH60_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNQ16827.1"
                     /translation="MLTEAERRAVVFLAAVDGIGSVTLRRLIDWASEKQVSLASIVGK
                     PEKYSFFGQNAKLLDRIYQLKQQHSIETYWEWLLSENIGVLFSENLDYPNLLKQVEGH
                     PFVLYFRGKVDVLQANKPIAVVGTRRATAYGERATQAIVSELVQHGATIVSGFMYGID
                     TIAHRTAMGSGGATIAVLGFGFGQMFPSSHGQLFEQMLQKKAIFISQFAPNTPASPGN
                     FPARNRLVAGISIATVVVEAALPSGSMITANFALEEGRAVCAVPGPFDNPFSQGTKWL
                     INQGATLVASGNDILVDAGLLSSQESVGSTSIPNNQLENLNLIQKQLVSLIAGGVRLS
                     DELADQLSLPLEQLSFELTQLEMLGIVRREQDGWVK"
     gene            10275..12437
                     /gene="topA"
                     /locus_tag="PKH60_01870"
     CDS             10275..12437
                     /gene="topA"
                     /locus_tag="PKH60_01870"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNQ16828.1"
                     /translation="MQLVIVESPTKAKKLRQILGSGYEIEASVGHVRDLPKKKMGVLV
                     DQDFIPEYEISADKTKVIRLLKDKVAKAHSVILATDPDREGEAIAWHLQQVLQTKSGK
                     NTKQIEFARSSFHEITQQAVLAAINNPSVINMALVEAQQARRVVDRLVGYEVSPVLWR
                     KVRRGLSAGRVQSVALRLIVDREREIEAFKPEEYWEVDVTLSTEGNSQSRELFVTRLV
                     SLNEKKYLPVSEKDVLSPRDWLPVATYKVLSVEKKERTVASLPPFTTSTLQQAAANRL
                     GMTSKQTMRFAQQLYEEGLITYHRTDSVNLAAQAVEQARAYINTTYGKAYVPDKPRYF
                     ANRSKNAQEAHEAIRVTDVGLADAMSVGGQITDRHQKLYDLIRRRFLASQMSAAVFDQ
                     AVVMVQAKSGNQVAELKANGSVLKFAGWKELFPVQEDVTLPSLEADQVLTYEDMQTLQ
                     KFTQPPARYNDASLVKELEKQGIGRPSTYASIISVIIDRGYVERTEKRFYPTSVGISV
                     CDFLLANFKTFMEYDFTAEMEEDLDRISRGEKEWVSVVRSFYGPFHQAVEKASTDAER
                     VKIPVESTGEKCPECGEGEVVIRTGKYGKFKSCSRFPECKYTKNIVETVAGVVCPLCQ
                     KGEVIARKSRWGKLFYGCATYPTCTWASWQAPKPGETLTQAEWDERQAKRAERKANRA
                     GGAEKTEEAAKSAKKKKKTVAKRMPTKKKPVKPKRKAS"
     gene            12457..13059
                     /gene="nadD"
                     /locus_tag="PKH60_01875"
     CDS             12457..13059
                     /gene="nadD"
                     /locus_tag="PKH60_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNQ16829.1"
                     /translation="MRITLFGAAFNPPQLGHQQIAVSLLKEKLADAVWLVPVKEHPFG
                     KKLASASDRLAMTQLLVQSIEDELKKTSEFTSQSAVRVETFELDEPGISYSYRTLLAL
                     TERYPEHQFSFVIGSDNLSTFHKWDQYEAMLAQFPFFVYPRHGFPFVPLLAGMTPMQN
                     MAEVRVSSTQIREQLKVDGVISELMPKAIAEYIKDHNLYR"
     gene            13089..13916
                     /gene="nadE"
                     /locus_tag="PKH60_01880"
     CDS             13089..13916
                     /gene="nadE"
                     /locus_tag="PKH60_01880"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNQ16830.1"
                     /translation="MIGEILSFIKQTFTKTGKQTAIVAVSGGIDSAVSLSLVVRALGA
                     DKVHVVLLPYGEQDMTDARAVMDFNQIPTSNQHEIQIKPIVDSIFDVVDGKNKNVELR
                     LGNIMARARMIMLYDLAKTLDALVCGTENKSEKYLGYFTRFGDEASDLEPIQHLFKMQ
                     VRQIATELQLPQEILTKKPSAGLWQDQTDEAEFGFSYHDADKVLEIIVDKQPALLERL
                     TNGKARSLEEVRDGARFLSGASLDPTTIEKVLKHVQANWFKHQVPYHMESEQQSVFK"
     gene            13954..14373
                     /locus_tag="PKH60_01885"
     CDS             13954..14373
                     /locus_tag="PKH60_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16831.1"
                     /translation="MKFISKFILSTERVIRWFIFIITVFVMFYLVVHSLMMFFATSTP
                     QADIPRSGYDFLSTSIEGWKVYRNLMYLLLILDLPLVGLSVFIKEKSGGIVSALKSVG
                     IWFGLLNLVAFSYALSGLVFDLCMVGYTFFILVQKPT"
     gene            complement(14398..15060)
                     /locus_tag="PKH60_01890"
     CDS             complement(14398..15060)
                     /locus_tag="PKH60_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16832.1"
                     /translation="MKSTELPPVVDRLIDPLKKETVLLSALTDFTQNYSEHLISLVTE
                     SGESAFDTDVIQEHQHALNCIETLTQFNDEITNGQKAQLWWCAEDLIIHLRMYQPELV
                     SSHHELFNLLNAIQHLLEPNFPRLSKKAQREQLCITWFRKQFELKNIIGPESINGGSN
                     TLGRWIKAITESLEADNPTKAQNLSAHWAAGGLLELIAKTKNPLLNEFVPLLKQIRHL
                     AK"
     gene            15133..16032
                     /gene="thyA"
                     /locus_tag="PKH60_01895"
     CDS             15133..16032
                     /gene="thyA"
                     /locus_tag="PKH60_01895"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006141911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNQ16833.1"
                     /translation="MAEKKPDTQYRDLLKLVLEKGMRSEAQQEVDTISLIGAPPMRFR
                     LSEGFPMITERNMAPKLKSGDATVWQFAIAELIGFINGARTQAELEKYGCNWWKPWVT
                     EAKCSKRGLETGDLGPGSYGAAFHDFPTAEGKPFNQFKEILEQMRELPHLKTHFISPW
                     IPQYTIRGKGKTQKVVVCPCHGWLHFRIFDNKLIVHMFQRSGDVPVGVPSNMVQYAAL
                     TMMVAEVLGLEPYEYVHTISDAHIFVDQVPAVEEMLKREPLPLPTMRLSTKRDDLFAY
                     RYTDFELLNYHPHPGIKNIPVAI"
     gene            16067..16540
                     /locus_tag="PKH60_01900"
     CDS             16067..16540
                     /locus_tag="PKH60_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNQ16834.1"
                     /translation="MSKTTPKFVDFDNARYEDQAAVMAQSEAAGVCPFCAENLGKFHQ
                     SPIEWEGKHWVLTKNGWPYVNTKVHLLAILKLHQESIDQLSDAAAAELFTALKWAVAK
                     YQIPGGGLVVRFGDSNYSAGSVLHLHAHIIQPDIYAPNYAENPVKVKIGKYKKTS"
     gene            16567..17271
                     /locus_tag="PKH60_01905"
     CDS             16567..17271
                     /locus_tag="PKH60_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HNQ16835.1"
                     /translation="MRLAFFDVDYTLYKGFTVVQFFKTFAENNHRLDLISERKLLETE
                     VKHGTIDHQRLVEWSMSLAARAVEGFSVDKVALEIKKTIDAEGEFFPWVGPLLLYLKE
                     KNFRVYLISASIEPMIAEIALKLEIHHYFATMLEVVNGIYTGKVLYVRNGQSKLDTLN
                     SIIKDISEQNDVIAFGDSDGDVAMLSAADQGFVIDPVEYSDKLLATARKKGWPILRHD
                     TALEQIKQTLTEKFGI"
     gene            complement(17281..18183)
                     /locus_tag="PKH60_01910"
     CDS             complement(17281..18183)
                     /locus_tag="PKH60_01910"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HNQ16836.1"
                     /translation="MKALIAYIPVWHAGYQQFFESVIDKVEKILLIDSAWAQTLSPDM
                     GYLRKEIRALSTPSAQQLLIGLYPDLAVEVLNGATVSSDSSKQIVSIVAPDEDVSRFV
                     VEKFYPTIPVEYSPIFLRWDRTKATEEKPIVPNTSVTVNELTNQMFTEAYWAAEQSSD
                     WWRHVGAVLTKDDVILFSAANRHKPTDMTPYIVGDVRQLFHQSEYMNYSTAEHAECAV
                     IAEAARRGVATIDASLYVTTFPCPYCARLIAHAGVAELYFAEGYATLDGEELLKQAGV
                     EIIQVENIVVEKKSRSILKPYPKK"
     gene            complement(18186..18743)
                     /locus_tag="PKH60_01915"
     CDS             complement(18186..18743)
                     /locus_tag="PKH60_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNQ16837.1"
                     /translation="MKQKNGIVLSTAQISSLVSRYRKEHHTIVLTQGSFDLIHIGHGR
                     YLDKAKKYGDVLFVGVDSDEKVRKRKGPDRPVVPESERLEMLTYLKSVDHVVQKELTA
                     PKWQLIKLIQPDVLIATASTYTPTQIKQLKQWCGKVVVLKSQATTSTSAKLRRLQIGF
                     VQAFSENLTQKVQQAIGEVFAELKQ"
     gene            18829..>19169
                     /locus_tag="PKH60_01920"
     CDS             18829..>19169
                     /locus_tag="PKH60_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16838.1"
                     /translation="MQFLTEQVATESRNQMIMPATRNDQLRLARVEQLQFEQHLQKVR
                     RFLEKRPLMEWADSRPETVLRLILSEIEESLVELLSDIVQGKASDKLRIKVETALLQL
                     RSFPLHSEEQY"
BASE COUNT         5712 a         3534 c         4376 g         5447 t
ORIGIN      
        1 tgagggaaat acttcatcta gctaaaattg gtttgcaccc atcaaatttt tttagtcctc
       61 atattacgat tggtggtcta gatttatccc ccgaccatca agagccgctc tacatacaat
      121 cattagtatt tggttgtgga ttttctgaat ttaaacaagc cgatctcaat cttcgacggg
      181 aaaaaggtaa atttaaaatt aaattaagag aaagatataa aaaaataaca gagacccctg
      241 atggtatcca atatgttccc tttttaaaaa aacgaagcgc tgaatacaca aaaccatacg
      301 gtcaaaaaca gacggaacaa gatgaggata gtttgcgtag tgttgagttt cgattgggta
      361 ataggtttaa gagtttccaa aatctcacca gagaagttga tgctatctat tacctatcga
      421 cagctgccat agcgcatcaa aaggatgcgt cagagagaga tcctgttgag gtaaaacttt
      481 ctcaaatttg ggaggagttg gcacacaaac tagatgaaat atatctacag attggtatac
      541 attttccatc tggtgcaacc aagtcgttta tctttggttc ttcagattct gaagaagtga
      601 aggcaaaaag gggatctacc ccgtacaata ctcacttatt cgaactgggc taccaatctg
      661 cgacagacgc tgtttttcga tcgcagatcc gtactttagt taagaacgca aggaaagaaa
      721 ttttggactg cattaattgc cgataatgca aggtttaaag attattaaga gctgcttcca
      781 tacttgcaac tttggctttg agaaccgtat cgtcaggact tatatatttt agaatatagt
      841 gatattgctc gagggcagct tgaggattgt ttcgtagttc ataaatggtg gccaaggtac
      901 ctctcgcctc ggcgtaattg ggttttagtt ccagggcttt ttgcaagcta acaatagcct
      961 gctcatattc tgattggtaa cctagaattt ttccttctag taaccaaggt cgcggatcag
     1021 ttggggataa tgttttagct ttttctacca cttcaagcgc tttggttagc caggccaaat
     1081 ctagtggagc caactgcatg tacgagccaa ttttagttaa gtacatggtg tagtggacag
     1141 gatttacttg ggtgctattc tcgagtaagg ttgctgcttg gctcgcaaga gtcgcagctt
     1201 cacttgcttg ctggccagct gcaagctggc cagcaatatt tgcataatag ccaccgagcg
     1261 tggagtaata actggcctct tgtggtctga gcgcaatggc tgaaagaatt ttgtctgatc
     1321 caatagctaa ctgaccttgt tgaatggtcg ttttgccctc agtgtagagc acgtcagcat
     1381 gacgaattga tagggttttt ccgaccatta ctattccgag gataccgagt ccagataaaa
     1441 ataggtattg ggtaacactg agtgatttgc tgttgttggt agaagtaagc aatggttggt
     1501 gaccccagat tattactaac actcccatga acaagaattg tatnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnntacaaaa agtcccactc ggagaccagg ttgtgtgtgg
     1681 tcggtcggtc ttgataatag ctgtaggcaa acgtttctag acctgatccg aaaaatggat
     1741 agcgtttcca aacatcgatt gccccttgcc agacaatttt tctaatctca cctgactcag
     1801 tcccacctgc ttcaagagcc ggaccgaacg tttcaacttt tggtgcttcg gatttattca
     1861 ataactgcgt aatagaggga gttagcggag taccgaaata cagcgtgctc gtgaggagta
     1921 gaaagccaat aagactgaat cgaattagta tttgtttgag cgcaactttt ttgattgacc
     1981 aggagtgcgt gaaatatgca agcgcaaacc agcccaacaa gaagactaaa cttactccaa
     2041 atcctaataa tcccgaacga gatttggtga acaatacaac acagtaccaa agcgtggtaa
     2101 tgctgagtag actgatccag accagacgtt ttttcatgaa gagtagagaa ctaatgccga
     2161 ttgggattag tagaatcaga taagcggcta gccagtttgg ttggccgaaa ctgccaaaaa
     2221 cgcggttttg tacatcttga acccagcaac taacattgaa tgcaccagtt gcaagctgac
     2281 agctaaaaga gtatccaaag tgttctggca gagcatacaa gcttgcgagc actgagccaa
     2341 taactagact caatagccag tgcgcaatcg gttgtgacaa aaagggagtc ttttttgatt
     2401 gggagtttcc aacaaaagta gctacaaagg cataaaagag cgtaacatag gccagagttg
     2461 aaaccaaacc accatgaaat cttgagtagt agccaaacag agatgtgtaa ggatgaattg
     2521 aaaacagtgt ggccaaaagt tgagaaacaa tgtagccaat tattggaagg tcaaatgggg
     2581 ttttgactag gagtactttt ttctgaacga tcatgcgagc taaccaaata agagcggtga
     2641 tgatcgccca aatatacacg aagatcatct tgttaaattc aaagagctct tcggtgttga
     2701 aggcaaaaac taatggcaac gccagagcac caaaatgaaa taatgagaca agcgctcgtt
     2761 taagccaacg atcaagagaa ttttccatag tttgcagttt ctaactttct tgctatagtg
     2821 gctgtactat aatttttaag gtagtgatat gcaagaccaa cctgtccaga atccaacaag
     2881 taatcaagat ccacaatcgt atgcagatgc atatgatcac ccaacccagt caccagttgc
     2941 caattcagat catgatgttg tcttacctcc accagttagt cagaatactg tttctgcaag
     3001 tactacctca gaaacattgg aagatcaaaa cattttctac ctgcttgggg ttgaagacgg
     3061 caccaacgag caaaaagaaa cctttttaga cgaacttcag caagtaattt gggaagattt
     3121 cttggaaaat gatgtgcaat tgttactaac gaaagaagag caccagcaac tgcagcaaat
     3181 gactaataat gacaccaaaa aacccctcat ggaacaggaa gctgtggtcg tatttttgga
     3241 aaaactcatt cccgatttag aagaaataat gttagagaaa gcgcttgaac taaaagcaga
     3301 tttagtcaag gaacgcgttg cgggcttgaa agaattgcat gctggtaaaa ctgatatgtt
     3361 acagaagatt gaagaagcag aaaatcttgt tagtcaagat aaatggttga gtgcagctcg
     3421 aatgcttaat tcattaccag catagtgttt tggtcgttat aatagcacca cgatgagtct
     3481 ttctagtatt gttgctcgga ttgtgccaag tggagctttt cctccgttgg ttattgattg
     3541 tggttcggcg tataccaaag ttgcatataa aggcagattg atttgggaac agcccaccgt
     3601 ggttgctatt gatcaacaat ctgatagata tgtttcaatc ggtacagtgg cggttaggca
     3661 attggactta gatgctcaac gacatttaag agctgtctat ccaattcaaa acaggatggt
     3721 tgcgcacatg gagggactcg aatggtatat aacccaatta ctaaatcaga ttacttgggt
     3781 tgacggatcc tcaatatcaa tttttacacc attgcttttt attgttccta tgggtcaatc
     3841 gagatcgaat agtaagcaat ggttgaaatt tactactacc ttgggtagaa aaatttcttt
     3901 tttgacagaa gaaatagtcg tgagtaagtt gcaaactgag atattcttgc caacttggat
     3961 cattgatttt gggtatagtc atacgactag tgcgttttat gatgaggatg gatatgttgc
     4021 tagcaactct tttccctggg gattaaatag acttattaga gctcttgagc ggtctgtcac
     4081 acagcggaat atacatttaa gttcagaaca aattgagcag gtactccagc aacttgtatt
     4141 tgtaccaaca atagatggtt caataaaaaa gcgaaaaata ggactctctg ccaaacaaat
     4201 aggaaatggc tcaagtgttc gcctcatact taatcaagaa gatgttgaat catcagtcgt
     4261 ggaaattgtc gttgagtggt tgtggctcgt taaacaacag atggtgcaat tatcacaaac
     4321 gattgaaagg gatttaacca agacaaaatt ttattgtttt ggtggtggct cattacttac
     4381 tgggttaacc caaaacattg agcgaacact ttctatgcaa atgcaaacta atttagtagc
     4441 agtaacaagt ctgccaccgt tggtgacaaa atagtatgtt cacactaaat aaattatttg
     4501 ctagggttgc tatctatttg attacctgta ttgcaggttt gggagtagcc gagctgactg
     4561 gttcagctca ggaaattcaa actctggtac ggcaaaatag tatcccgatt cagatagcca
     4621 cggctcagag aatccaaagt atcaagacac caatttcatc tctttttacg ttttggaatc
     4681 agttagaaac ccaccgagag ttggaattag catatgcaaa cttgcaggcc cagttggtac
     4741 atttagaaca attagagcaa gaaaaccaag aattgcgttc gcaacttggc actgaatctg
     4801 agaaaaaaac tcaaggactg attacaacgc cagtattatc ttatccgtat agatttatta
     4861 attatggagc gcaatcagaa gtcaaaacgg gagatttagt tttagtaaac ggaacgttag
     4921 ttggcagatt gggagaagtt aatcttgagt attcacgagt tattttattg agtgatcaag
     4981 atgcctctgc aataattggc atcaccaatc ttggacaaag gggtgtggtg cgtggcagtg
     5041 accagcaact tgttttgacc gaaatacccc aaacagcaac tattacattt ggtgatagag
     5101 tgatgtcagc tggagaaaaa ggagtaaaac caggtgtatt catcgggacg attgggaaag
     5161 aattgaccaa gcccactgac gcagtaaaaa cctttgtgat agatcaagtt aatacatttg
     5221 ataccgcaag attggtagaa attcagtaaa gaactatgaa acttacaatt caactacgat
     5281 tacttttctt gttactcctc aagcttagcc tagtttattt tgcactttta ttagattttc
     5341 aattgggact tccaatgctt acttgttttt tactactgag gttttctcgt ggaactaacc
     5401 aaccggtctg gataattatc tatctgatgc tttgctcact tatgtttagt agcatgtttt
     5461 cactttcatg gttgatcaca ctaagtgtgt ttggtgcagc ctttcttttt gatcattcaa
     5521 tgagatttaa cacgaaacaa tcgaggttct tattactggg gctatcaatc tttataagta
     5581 ttttgttgtt cgttgtggca ggaggaatgc tatcgggagg tgtagtactg tatgggatta
     5641 tttctctagc tacgattatt ggaataagtc aatggagttg gttttcgcga tttcaatcac
     5701 agtcgtggta tattcggtag cacagtgtta gagcagaatc ccaatacttt tttttggaga
     5761 gcacgaatat ttttgagcgt attacttgtt agcgtgatcg tggtgttggc tcgtttggtt
     5821 gatttacaac tcgggcaggt tggttggtat caggcagctg cccaggataa tagtgatttt
     5881 agattgtccg ttcctgccaa tcgcggtgtt ttgttagatc gatatggtga ggcgttggtg
     5941 aaaaatagca ttcggtattg gcaaaaacag gatttaacaa catttttttc aaaagaagtt
     6001 ttggtggatg atagttccgc ccttggattg ttagcaaccc aggctgctca aattcgaaag
     6061 gaatattctc gttggtatcc acataattca tcaggtgctc acgtgattgg ttatgttggt
     6121 ccgatctcaa aagacgaatt attactcaat gaaaatgctg aaataacaga cttagttggt
     6181 cgtactggtt tagaacgggc ttttaataca ttgctaacag gtactgctgg agaaattgaa
     6241 tatgaggtca cagctttggg tgaagctaat cgagtgatac aagaaaaacc aatgattccc
     6301 ggtgctgtta tcaaaactac gctagaccca tatcttaccg ctatagctca gaaagcgatg
     6361 gaaaataata aaggtgctgt aatcatagcg gatgcaaaaa ccggggctct tttagctgta
     6421 gttagttcac cttcatatga tccaaatgtt tttaccaaat ttactcaaac caacgaggag
     6481 caagccttaa gacggcagca aattacatcg tttttggagg atccagacaa ggcgttgttt
     6541 aatcgagcca tagcaggcga gtatcctcca ggatctgtat ttaaattagt tacggcgatt
     6601 gcaggactag aatcggagag cattacccca agtacggtgg tgaaggatga gggaatttta
     6661 aaagtgggtg agtacgcata tgcgaactgg tatttttctc agtatggtgg aacggaaggc
     6721 gagatcagtt tggagcgagc cttggctcgc tccaacgata tctattttta caaagcggct
     6781 gagttcacgg gtcctgatgc aatcgcaact tgggcgcgaa atattggatt tggttcccca
     6841 acgggaattg aattaaccgg agaagcaagt ggtttagtac cagacccgga atggaaagaa
     6901 cgtactctgc atgagcaatg gtacttgggt aatacatatc actatgggat cggacaggga
     6961 gatctgctgg tgagtccaat tcaagtttct cagttatttc aaaccattgc taatcaggga
     7021 gtgcgttgtc aaccacacat ttctgaagat ggtaagaatg agtgtcattc attaggtatg
     7081 aaagaagaaa atgtaacacc gattctgaga gggatgattg ccgcctgtag taccggcggc
     7141 actgcgtttc cattttttgc atataacgaa cagtttgaac ctaacccaaa cccaaatgag
     7201 gcaattgcga acggtatggc agcgtgtaaa actggtacgg cagaatttgg tggagcagac
     7261 gaacggggat ttcgtaaaac ccatggttgg ttttcagttg tggtgggtgc gccccaatta
     7321 aataatccag ttgtggacgg agaacttgag acgttaaatc tagaggatca agccagaaac
     7381 gaatggttgg caaaaatacg gcaaaatcct ttacccaaag aattgataat tacagtgctg
     7441 gtggaaagtg acgaagagaa accatacaaa gaaggcagtg ctgatgccgc gccaattgca
     7501 ctggagcttc ttgagtggat gaggggaaag taaacagcgc tagtaaatta tttgttatga
     7561 taatgatatg gagcaagatt cggatcagtt agttctgccg ctaataccac aggaatatct
     7621 ccaaattttt ccaaaagtgg tgcaactaat tgaagagttt ggactactac ctccaactga
     7681 gcaaacatac ttagaagtca aaaagatggc tgaacaaggt ttgccacttg taaaaagctt
     7741 atacgaagat gctactttgc atttattcaa ggaaggcaaa ttattatctg aacatgacag
     7801 acaaggtgaa ggatttaatg ttatttacgc ttcaattttg ttgaataaag gatatggacc
     7861 aataacccta gtgttatccc ccgaaattat gatggagggt agatttgaag cgtactgtat
     7921 tgatcacgcc agtacatacc tcagcgaccg ggtaaacgac tatatcaatg agcacatgag
     7981 tagcagtgta gtaagctcag ccgaaataca agatatagaa aatgatttaa tggaaaaata
     8041 tacactgaat ccccaggaat tttttgtttt ttactcatat tgcgttgcaa gtctttgtga
     8101 aaatgaagaa gagtattttt atgacgattc ttatgaagag aggctgagat ttcttacttt
     8161 cgatattggc ctacctgaaa taaaagtgga tgatttgcta gcagtgatat gcaagcaggg
     8221 atggggagat caagttgtag acgaagacaa tttaagagat atttgtactc aaaaagataa
     8281 ggcattttta actgttggaa aaagagatta tgggtcacag tctctgtaca gtcaagaggt
     8341 tgctattacc tcaaaattga tggaacaatt tcaaattccg atgaaatatc gagagaagat
     8401 gaatcaagta gcatagcctt gtatcactat gaaaaaaaat ttacaaaact gtttatatcc
     8461 aacgacattt ttgattgtta gatacttatt atatgtggca gttgcaacta gagttcaata
     8521 tattttattc ttgagtaacg ctatcaaatt tcaaatcaga aagagactca atcaccaatt
     8581 gtttaccatt ggcgccttca acaccagaaa ctaaaattcc aacaacttca tcttgttctg
     8641 taatggctgg agagccgctc atgccaacct gggcgtcggt gccgctaagc cactcagcaa
     8701 ttactcggcc attgggttgg acttcaatca ctctaacttt aatttcaaat ggtacatgtt
     8761 gaaatccatg aaaaccaaca atagttaagc actgatcgag gctggggggg gcgattttct
     8821 caaaaccagg tgaaacagtt gtaactgttg aaattgctag atctctgcca gctatcagtt
     8881 gaagaggaat atcggtcatt tgatccaagt gaggcgtatg gtatgtaggt gaaccacttg
     8941 caacatgtcc cgcagttaca atgtgagctc tcagaatgag cgccatggcg taaccacaag
     9001 gatcctctgt cagaagcaag ttgttggctg gaaaattttc aggatgagtc attccaagat
     9061 tgaattgtag caataattat ttgcatattc acgaggctct acggttaatg tctgttttca
     9121 tttgtagtat gaccgtatgt tgactgaagc agagagaaga gcggtggtat ttttggcagc
     9181 ggtggatggg attggttcgg ttactttacg acggttgatt gactgggcaa gcgagaaaca
     9241 agtttcattg gcatcgattg taggaaaacc agaaaaatat tccttttttg gtcaaaatgc
     9301 caaattgtta gataggattt accagcttaa acagcaacat tcaattgaga cctattggga
     9361 atggttacta tcagaaaata ttggagtact attcagtgag aatctggatt atccaaactt
     9421 gcttaaacag gtcgaaggcc atccgttcgt gttatatttt cgaggtaaag tagatgtttt
     9481 acaagcaaat aagccgattg cggtggttgg tacgaggcga gcaacggcat atggcgagcg
     9541 agccactcag gcgattgtga gtgagttggt gcaacacggc gccaccatcg tttctggttt
     9601 catgtatggt atagacacta ttgctcatcg gactgcgatg ggctctggtg gcgccaccat
     9661 cgctgtgctg ggatttggtt ttggccagat gtttcccagt tcacatggtc aactatttga
     9721 gcaaatgctg caaaaaaaag ctatattcat ttctcaattt gcacccaata cacctgccag
     9781 ccctggcaat tttccagcta ggaatcgttt ggttgctgga atttctattg caacagttgt
     9841 ggtagaagca gcgcttcctt ctggaagtat gattactgcc aattttgccc tagaagaggg
     9901 aagagcagtt tgtgcggtgc caggaccatt tgataatcca ttttcccagg gaaccaagtg
     9961 gttgatcaac caaggagcaa ctttagttgc gtcgggaaac gacattttgg tggatgcggg
    10021 attgctttct tcacaagaaa gtgttggtag tacttctata cccaataatc aattggagaa
    10081 tttaaactta attcaaaaac aacttgtttc attgattgca ggtggagtcc gtttatcaga
    10141 cgagttagca gatcaactct cgttgccatt agagcaactt agttttgaat taacccaatt
    10201 agaaatgtta ggaattgtca ggagagaaca agatggttgg gtaaagtagc tatctgttac
    10261 aatgcaagtt tgttatgcaa ctagttattg ttgagtcacc taccaaagcc aaaaaattac
    10321 gacaaatttt ggggagtgga tacgaaattg aagcttcagt aggtcacgtg cgtgatttac
    10381 ctaagaaaaa aatgggtgta ttagttgatc aagattttat accagaatat gagataagtg
    10441 ccgataaaac aaaggtaatt cgtttgctta aagacaaagt ggcaaaggca cattcagtca
    10501 ttttagcaac agatcccgac cgcgaaggtg aggcaattgc ttggcattta caacaggttt
    10561 tgcaaacgaa aagtggaaag aataccaaac aaattgagtt tgctcgttca agttttcatg
    10621 aaattactca gcaagcagtg ttggctgcaa tcaacaatcc aagtgtgatt aatatggctt
    10681 tggttgaagc gcaacaggct cgcagagtag ttgatcggtt ggtaggttat gaggtgtctc
    10741 cagttttgtg gcggaaagtc cgccgcgggc tttccgccgg ccgagttcaa tcagttgcat
    10801 tgcgtttaat tgttgatcgc gagcgtgaaa tcgaagcttt caaaccagag gaatactggg
    10861 aagttgatgt cacactgtca actgagggca attctcaatc gagagaacta tttgtcactc
    10921 gactagtttc gttgaatgaa aaaaaatact tgccggtatc ggaaaaagac gtactttcgc
    10981 ctcgtgactg gttaccagtt gcaacgtata aggtcctctc ggtagaaaaa aaagagcgca
    11041 ctgtagcgag tttgcctcca ttcactacat caacattaca gcaggccgct gccaatcgac
    11101 ttggcatgac gagtaaacaa accatgcggt ttgctcaaca attatatgag gaaggcctga
    11161 ttacctacca cagaactgat tcggtcaact tggctgctca ggcagttgaa caagctcgag
    11221 cttacatcaa cactacttac ggtaaagcct atgttccaga taagccacgc tattttgcta
    11281 accgcagtaa aaacgcacag gaggcacatg aagcgattcg tgttacagat gtaggactcg
    11341 ctgacgccat gagtgtaggg gggcagatta ctgaccgaca ccaaaaattg tacgatttaa
    11401 ttcgccgccg ctttcttgct agccaaatgt ctgcggcagt ctttgatcaa gcagtggtaa
    11461 tggtccaagc taagtctggg aatcaagtgg ctgaattgaa ggctaacggt tctgtactca
    11521 agtttgctgg ttggaaagaa ctttttccgg tccaagaaga tgtaacgcta ccgagtcttg
    11581 aagctgatca agtacttact tatgaagata tgcagacttt gcaaaaattt acccaaccac
    11641 ccgcacgata taacgatgct tcactagtca aggagctgga aaaacagggc atcggcagac
    11701 ctagtacgta tgccagtatc atttccgtaa ttattgatcg aggttatgtg gagagaactg
    11761 agaaacgatt ttatcctaca tcggtaggca tcagtgtgtg cgatttttta ctcgccaact
    11821 ttaaaacgtt tatggaatat gattttaccg ctgagatgga agaagatttg gacagaattt
    11881 ctagaggtga aaaagagtgg gtatcagtgg taaggagttt ttatggaccg tttcaccagg
    11941 cggtagaaaa agcttcaacc gatgcagagc gagtgaaaat tccggtagaa tcaactggtg
    12001 aaaagtgccc agaatgcggt gagggggaag tggtaattag aactggcaag tacggaaagt
    12061 ttaaaagttg tagtcgattt cctgaatgta aatatacaaa gaatattgtt gagactgtgg
    12121 cgggggtagt atgtccactg tgtcaaaaag gtgaggtgat agctagaaaa tccaggtggg
    12181 gcaagttatt ttatgggtgt gctacgtacc caacttgcac ttgggcgagt tggcaggcac
    12241 cgaaacctgg ggaaacactt actcaagccg aatgggatga gcgccaagcg aaacgcgcag
    12301 agcgaaaagc caatcgagca gggggtgctg aaaaaaccga agaggctgcg aaatcagcca
    12361 aaaagaagaa aaaaactgtt gcaaagcgaa tgcctacgaa gaaaaaacca gttaaaccaa
    12421 agagaaaagc aagctaataa gcgcgcttag taaattatga gaattacttt gtttggcgcg
    12481 gcatttaatc caccacaact gggtcatcag caaatcgctg ttagcttgtt gaaggaaaag
    12541 ttggcagatg ccgtttggct ggtgccggtt aaggagcatc catttggcaa aaagttggct
    12601 tcagctagtg atagattagc aatgactcaa ttattagtac aatcgattga ggatgaacta
    12661 aaaaaaacta gcgagttcac aagtcaatct gcagttaggg tcgaaacatt tgagctggat
    12721 gagcctggta tttcgtatag ttatcgaaca ttgttagcac taactgagcg atacccagag
    12781 catcagtttt catttgttat tggttcggat aacttatcca cttttcacaa atgggatcag
    12841 tacgaagcga tgttggcaca gtttcctttt tttgtgtatc ccagacatgg atttccgttt
    12901 gttcctttgc tcgctgggat gacacccatg cagaatatgg ctgaggtgcg agtttcctca
    12961 acccaaattc gtgaacagct gaaagtagat ggggtaataa gtgaattaat gccaaaagcg
    13021 attgctgaat acataaaaga tcacaatttg tatcgataga tttatagatt ggggtagaat
    13081 agctaagtat gattggcgaa atacttagtt ttatcaagca aacgtttaca aaaacaggta
    13141 agcaaactgc gattgttgcg gtttctgggg gaattgattc ggcggtcagt ttaagtttgg
    13201 tagtgcgcgc gctgggcgct gacaaagtcc acgtagtact actaccatac ggcgaacaag
    13261 atatgactga tgcgcgcgct gttatggatt tcaaccaaat tccaaccagc aatcagcatg
    13321 aaatccaaat taaaccaatt gtggactcta tttttgatgt agtagacggt aagaataaaa
    13381 atgttgaact tcgtctgggc aatatcatgg ctcgtgccag gatgatcatg ttgtatgatc
    13441 tcgccaaaac gctggatgcg ttggtgtgtg gcacggaaaa taaaagtgaa aaatacttag
    13501 gttactttac tcgttttggc gacgaggcga gtgatcttga accgatccag cacttgttta
    13561 aaatgcaagt acgccaaata gctactgagt tgcaacttcc gcaagaaatc cttacaaaaa
    13621 aaccctctgc aggattatgg caagatcaaa ccgacgaagc agaatttggt tttagttatc
    13681 atgacgcaga caaagtgctg gaaattattg ttgataaaca acctgcctta ctcgaaaggt
    13741 tgaccaacgg aaaagccaga tcgctggaag aagtccgtga tggcgccaga ttcctatctg
    13801 gcgcctccct cgatcccaca actatagaga aagttctcaa gcacgtccaa gccaattggt
    13861 ttaagcatca agttccctat cacatggaat ctgagcaaca atctgttttc aaataaattg
    13921 tggtttcaga ctattactgg tagagtaggg gttatgaaat ttatttccaa gtttatatta
    13981 tccactgagc gagtgattcg ttggtttatt tttatcataa ccgtatttgt aatgttctat
    14041 ttagtagttc attcgctcat gatgtttttt gccacaagta caccccaagc agacattcct
    14101 aggtcaggat acgacttttt aagcacttct attgagggct ggaaggttta tagaaatctt
    14161 atgtatctgt tgctgatttt agatttgccc ctcgtgggtc tttcagtttt tatcaaagaa
    14221 aaatcgggcg gcatcgtttc agcactcaag tccgtgggta tctggtttgg tttgctaaac
    14281 ctagttgcct tttcttacgc gctgtctgga ttagtttttg atctttgcat ggtagggtac
    14341 acatttttca ttctagttca gaagcccacc taaatagtta gcttgggatg atggcaatta
    14401 tttcgccagg tgtcgaattt gtttgagtag tggaacaaat tcattgagta aggggttttt
    14461 tgttttagca attaattcta gcaatccacc tgcggcccag tgtgccgata ggttttgagc
    14521 cttggttgga ttatctgcct ctaaagactc agtaattgct ttgatccaac gacctagggt
    14581 attggatcct ccgttgattg attctggtcc aattatattt ttcaactcaa attgtttcct
    14641 gaaccacgta atacatagtt gctctcgctg agcttttttt gaaagtctag gaaaatttgg
    14701 ttctaataaa tgttggatgg catttagcaa attaaatagt tcatgatggg aggaaactag
    14761 ctctggttga tacattctca gatgaatgat caagtcttcg gcacaccacc aaagttgggc
    14821 tttttgacca ttggtgattt cgtcattaaa ttgagtgagt gtttcaatgc aattaagagc
    14881 atgttggtgt tcttgaatga catcggtgtc aaaggcgctt tctccagatt ctgtaactaa
    14941 gctaatcaaa tgctcactat agttttgagt aaaatcagtc aatgctgata aaagcactgt
    15001 ttcttttttt aacgggtcaa ttagtctatc tacaacaggg ggaagctctg tgcttttcat
    15061 agttatagta taccagagtg catcaagtta accatttgtt actacgggtt gtatgcatta
    15121 caatgccaac atatggcaga gaaaaaacct gatacccaat accgagattt actcaagtta
    15181 gttttagaaa aggggatgag atcggaagcg caacaggaag ttgacacgat ttccttgatt
    15241 ggtgcgccac caatgcggtt tcgtttgagt gaaggttttc ccatgatcac cgaacgcaat
    15301 atggcgccaa aattgaaaag tggtgatgcc acggtatggc aatttgctat tgctgaactg
    15361 attggattta ttaatggggc gcgaacgcag gcagaattag aaaagtatgg ttgtaactgg
    15421 tggaagcctt gggtcacaga agcaaagtgt agtaagcgtg gcttggagac gggtgatctt
    15481 gggcctggct cgtacggtgc ggcatttcac gactttccca cagcggaggg taaacccttt
    15541 aatcaattca aagaaatact agagcaaatg cgtgaactgc cgcacctcaa aactcacttt
    15601 attagcccct ggattccgca gtatacgatc cgaggcaaag ggaaaacgca aaaagtggtg
    15661 gtttgtcctt gccatggctg gttgcacttc cggatttttg acaacaaatt aatcgttcat
    15721 atgtttcagc gatctggcga tgtaccagtt ggagtgccta gcaatatggt tcagtatgcc
    15781 gcattaacaa tgatggttgc tgaagtattg ggcctggagc cgtatgaata cgtccataca
    15841 atttctgatg cacatatttt tgtggatcaa gttccagcgg tcgaagagat gcttaagcga
    15901 gaaccacttc cattacccac catgcgactc agcacgaaac gggacgattt gtttgcctac
    15961 agatatactg attttgagtt acttaattac catccccatc ctggaataaa aaacattcct
    16021 gtggcaattt agtcgttatg aatttgaaag agggtataat cgtactatga gcaaaaccac
    16081 gcccaaattt gttgattttg acaatgcacg ctacgaagat caagcagcgg taatggccca
    16141 aagcgaagcc gcaggtgttt gtccgttttg tgcggaaaac ctaggtaaat ttcatcaatc
    16201 accaatcgag tgggagggta agcactgggt tttaactaag aatggttggc catatgttaa
    16261 tacaaaagtt catttattgg cgattttgaa attgcatcaa gaaagtatcg atcaattgag
    16321 tgatgccgcc gccgcggagc ttttcactgc gcttaagtgg gcggttgcga agtatcagat
    16381 accaggcggc ggcctcgttg tccgttttgg tgatagcaat tattctgctg gtagcgtatt
    16441 gcacttgcat gcacatatta ttcaaccaga tatttacgca ccaaactacg cagaaaaccc
    16501 cgtaaaagtc aaaattggta aatataaaaa gacttcctaa tttttcagtc ttggaaagtt
    16561 ttaaacatgc gattagcttt ttttgatgtt gattacacgc tctataaggg atttactgtt
    16621 gttcaatttt tcaaaacatt tgccgagaat aatcacaggc ttgacctaat tagtgagcgg
    16681 aagctgttgg aaactgaggt taaacacggc acgattgatc atcagcgtct ggtagagtgg
    16741 tcgatgagtc tcgcagctcg cgcagtagaa ggtttttcag tcgataaagt tgctctggag
    16801 ataaaaaaaa caattgatgc agaaggtgaa ttcttcccct gggtcggtcc tttgctactg
    16861 tatctaaaag agaagaattt tcgtgtgtat ttaatatctg cttccatcga gccaatgatt
    16921 gcagaaattg cactcaagct tgaaattcat cactactttg ctacgatgct tgaagtggtc
    16981 aatggtatat ataccggaaa agttttgtat gtccgaaatg gacaaagcaa gcttgataca
    17041 ctgaactcca ttattaaaga tattagtgaa caaaacgacg tcatcgcgtt tggtgatagc
    17101 gatggtgatg tggcaatgct ttcagctgct gatcaaggtt ttgtgataga tccggtggag
    17161 tatagtgata aacttttagc tacagcaagg aaaaaaggtt ggccaattct taggcacgat
    17221 acagcacttg agcaaattaa acagacgctg actgaaaaat ttggtatcta aaatagacgt
    17281 ctactttttg ggatacggtt tgagaattga gcgcgatttt ttttcgacta cgatgttttc
    17341 cacttgaata atttccactc cagcttgttt aagcaattct tcgccatcta aggtggcgta
    17401 cccttcagca aagtagagtt cggcaactcc tgcgtgagca atcagccgcg cgcaataggg
    17461 gcagggaaag gtggtgacgt atagtgaagc atctatggta gcaactccgc gacgcgcggc
    17521 ttcggcaatc acggcgcact cagcgtgttc tgcagttgaa taattcatgt actccgactg
    17581 atgaaataat tgccgtacat caccaactat gtatggggtc atatcagtgg gtttgtgtcg
    17641 gttggcagcc gaaaaaagta ttacatcgtc tttggttagc acagctccaa cgtgtcgcca
    17701 ccaatcgcta gactgttcgg ctgcccaata tgcttcggta aacatttggt tggtaagttc
    17761 gtttactgtc acactcgtat ttggcacgat cggtttttct tcggtggctt tggttctatc
    17821 ccagcggaga aaaatcggtg aatattcaac tggaatagtg gggtaaaatt tctctactac
    17881 aaatctggaa acatcttcgt ctggagcaac gattgacacg atctgcttac tagaatcact
    17941 agaaacagtg gcgccgttca gaacttcaac agctaagtca ggatataacc caattaatag
    18001 ctgttgagct gatggagtgg agagcgcccg gatttctttc cgcaagtaac ccatatctgg
    18061 agaaagtgtt tgagcccagg ctgaatctat cagtaaaatt ttttcaacct tatcaattac
    18121 tgattcaaaa aattgttgat aacccgcatg ccagacggga atatatgcaa ttaatgcttt
    18181 cataattact gtttcaattc tgcaaatact tcaccaatcg cttgctgaac tttctgggtc
    18241 aggttttcgc taaaagcctg gacaaagcca atttgcaggc gacgcagttt ggcgctggtt
    18301 gaagtggtgg cttgagattt taatacaacc actttgccac accattgctt aagttgttta
    18361 atttgagtag gagtgtacgt tgacgcagtg gcaattaata catctggttg tatcaattta
    18421 attaattgcc acttaggtgc agttaattct ttttgaacaa catgatcaac cgactttagg
    18481 tacgtcaaca tttccagacg ttcggattca ggtacaaccg gtctatctgg acctttgcgt
    18541 tttctaacct tttcgtcact atccactccc acaaacaaca catcgccata ctttttggct
    18601 ttatcaagat atctgccatg accaatatga ataagatcaa aagaaccctg tgtaagaaca
    18661 atagtatgat gttctttacg atagcgagaa actagggaag aaatttgagc ggtagagaga
    18721 acaatgccgt ttttttgttt cacgaacaga attataccag ctttacacta ctacaaatag
    18781 tttttcaagt attgaaatac cactacatgt tgtggttagc taagaaccat gcagtttttg
    18841 actgaacaag tagcgacgga atcgcgaaat cagatgataa tgccggcaac tcgaaacgat
    18901 cagctgcgtc ttgctcgggt agaacaactt cagtttgaac agcacttaca aaaagttaga
    18961 agatttcttg aaaaacgacc actgatggaa tgggcggatt ctcgtccgga aacagtactt
    19021 cgattaattt tatctgaaat agaggaaagt ctggttgagt tactttcgga tatagttcaa
    19081 ggaaaagcat cagataaatt gagaattaaa gtggaaactg cattactaca gcttcggtcg
    19141 tttccgttgc attctgagga gcaatacaa
//
LOCUS       DAOQZP010000017        19024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_3267_length_19024_cov_4.910380, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000017 DAOQZP010000000
VERSION     DAOQZP010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 19024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19024
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3267_length_19024_cov_4.910380"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /gene="glgP"
                     /locus_tag="PKH60_01925"
     CDS             <1..1038
                     /gene="glgP"
                     /locus_tag="PKH60_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNQ16839.1"
                     /translation="SALRLIKNKIVYTNHTLVAAGNMSYPISALTPLAEPYANKMEIS
                     TQQLLSLGSSGNPDSFSVTSAALNVSHVASGVSQVHTDLSKQVWPDYNWTNITNGVHR
                     GTWQDSQIKLASEDSTNLWQRHYQLKEQLRDYVHATTGFGYDHHRMVVSWARRIAGYK
                     QLSALFSDIEALRRIATQQGREIQFLVSGKAHFGDSFSKDVLKEVIQHFSSDLSGYAL
                     FIPNYNIEIAQFLTKGSDVWLNTPIKGKEASGTSGMKAISNGVLQITVQDGWTSEVDW
                     NSVGWTLNPDDLTSSLYEMLQNAQNMYFQKNDRGFSEEWVSRMKASIELFDRFSTERM
                     LREYREKLYTA"
     gene            1035..1349
                     /locus_tag="PKH60_01930"
     CDS             1035..1349
                     /locus_tag="PKH60_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16840.1"
                     /translation="MTKNDRYFQLMSEKHAELFAAFDPIHQQFEKNRSNEDAFHNEGQ
                     KVVDVIRSWERRLCSGMERGQHAQYSQRLAEKFWTKVKVRYPLIEMVGLKSKTSSSFG
                     TP"
     gene            1346..>2463
                     /locus_tag="PKH60_01935"
     CDS             1346..>2463
                     /locus_tag="PKH60_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNQ16841.1"
                     /translation="MNIFFELSLLLTVTAIITLAVRKLRQPLVVGYILSGIIAGPYVL
                     NIVSSQHELELFSKVGIVFLLFIVGLYLNPKIIQEVGKVSLVTGLGQVLFTSLIGFAL
                     TLLLGIDRLAALYVAIALTFSSTIIILKLIADKKDLQKLYAKIAIGFLLVQDIVATFI
                     LIAITVVSGQSDMGLGSTLLLTLGKGMILFSVLGMFQRYILPKVTEYAAESPELLFLF
                     SLAWGTILASLFSALGFSIEIGALIAGVTLSVSPYSVEIASRLKPLRDFFIIIFFVLL
                     GSQLILSNLAQLLFPAIILSLFVLVGNPIIVIILMNTLGYNRKTSYQAGLTVAQISEF
                     SLILGALGLRLGHLSQEVLSLITLVGLITISGSTYLIL"
     assembly_gap    2464..2563
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2564..2857
                     /locus_tag="PKH60_01940"
     CDS             <2564..2857
                     /locus_tag="PKH60_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNQ16842.1"
                     /translation="PIVKPKIIISTVPELATNILIVHAMRAKNKKAVIIPIAQTHQDA
                     SELYEAGASYVMMPHHLGAQHMSKMIARYGSDHSLYETLKKRHVSELAAMFKA"
     gene            complement(2909..3337)
                     /locus_tag="PKH60_01945"
     CDS             complement(2909..3337)
                     /locus_tag="PKH60_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16843.1"
                     /translation="MGAFILPTAPELQHETAAGAELAEETLLAAIAGDLHEVSYFSFA
                     SDDYEGRKELRPVIVKAMELEYARSGANAFLFTGRTATNTRVFARATFSGINFQLSGW
                     QALSLGEMEGRSTGGSAESSFSINTSQVLEGSIYVLPSEE"
     gene            complement(3374..3946)
                     /locus_tag="PKH60_01950"
     CDS             complement(3374..3946)
                     /locus_tag="PKH60_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16844.1"
                     /translation="MKPFFALLLTIVMFSLLGCNNDAPPARKPEPCQKIVEYTVYGRN
                     DSILYHEVVQAELKFGEGQRRPNNSFLLYPLQEERFSESNARLQKIMGKANRNLTQFF
                     AGPPVTSMGGFYSTLEIGYHVDGTLVENVSQACQQPSSFTAWFGSGLEYYTGDTGHRY
                     RTSLKFVGETVYVTSALHETGSKVTYSFPA"
     gene            4176..4691
                     /locus_tag="PKH60_01955"
     CDS             4176..4691
                     /locus_tag="PKH60_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16845.1"
                     /translation="MKNLQAFYLVLVASYFMVGCTIQPNDLKTSEIQDPTPIPTTTAP
                     EPTASTEMYLGQQTNPSEAYEKLCAWVDKSDIIIELSFADGVVKDTSYEAGFAFSSIE
                     RTARLAREGCNYRTGFKFSELANKGEVQVAVWGYNDNRKRQLLNEPINLTFDSEGKPN
                     ISLPIQVTVLD"
     gene            complement(4700..5110)
                     /gene="mscL"
                     /locus_tag="PKH60_01960"
     CDS             complement(4700..5110)
                     /gene="mscL"
                     /locus_tag="PKH60_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNQ16846.1"
                     /translation="MSVLKGFRDYLFRGNVVDLAVAVVIGGAFGSIVTSLVKDIVTPI
                     IGAIGGQPDFSNIYFTVNNSKFMIGNFLNSVISFVMIAGVIYFVVIVPMNKIMAAMKK
                     EEKKDPTDKVCPECLSKIPINAKRCMYCTSILKK"
     gene            complement(5133..6008)
                     /locus_tag="PKH60_01965"
     CDS             complement(5133..6008)
                     /locus_tag="PKH60_01965"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNQ16847.1"
                     /translation="MTKSRIKIYIDDLIHKGLGYFIFSRLQKYRFTRRFVAIFKKNYV
                     QIEGYKLYINPMDTVVSATLAKTGTWEPFVSKQFIQIAKKARTIVDVGGNIGYFSLLG
                     AACSKSTAEIFVFEPDEVNFQSLTKTVKENKLENITCEKVAIAQKSGEISLYISPDNL
                     GDHRTFDSGDNRETEVVRVVSLDDYFSEYPHDIDLLKIDIQGYEHFAIVGANQLFQKK
                     RIKVILSELWPYGLKLAGCDWRNYLQILEESGFSLWEINEEDQIIRRLNKQKIADKIL
                     VEVEYSTTVLAVLNV"
     gene            6097..6861
                     /locus_tag="PKH60_01970"
     CDS             6097..6861
                     /locus_tag="PKH60_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16848.1"
                     /translation="MINENSNIQEDKADNDLERNLRLNRTHRLLAAAKQELVDAKIRL
                     SLVGANASGLNKIHTSLERQGAKSVVALLEYRILYLQQELVFIRAKNQADIEYRLLLR
                     REFSAKLSHRFGETPLLFHGANLITTLEIIKSKSISSTAERGLGQVSYDVDGSTSVSP
                     ADAVQVSLVDYTGIKDLFIPMGCLFVIKNESSTKNVSAIQSFSLFKDGQTNPCLIAIL
                     ASSESLPIIRSKLVECGYPAEIVQEYFAFLETQREY"
     gene            complement(7026..7096)
                     /locus_tag="PKH60_01975"
     tRNA            complement(7026..7096)
                     /locus_tag="PKH60_01975"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7062..7064),aa:Gln,seq:ctg)
     gene            7182..8666
                     /locus_tag="PKH60_01980"
     CDS             7182..8666
                     /locus_tag="PKH60_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16849.1"
                     /translation="MLQTEILQSDREVSRLYTAGLSEALDTNPPVSWWRSIAKKLIYF
                     ERHLSEIQSAPIRNHIQEKIAELKTILRNSQYLPMKSPLDIVEYTEKVIMSLSMEMAL
                     IFEEEREPITEHIISLDDLVHTYPHRLRKNESMLNDTPIIELEIRHPDPEIEQWYSIP
                     IPNQKMIWHKGGPARALLNLIADAPLSMQIAEFPWNDFDVITAGDDVYDTAISIGVDP
                     DGVEYMGENALNFSRFAYGRDTDQNQCCLGSDGLHYSTAALQAAQTGHINIVGKYIAN
                     KAIYGIDSMYAEGISLIKPRGLMRLVKNLVEGKALSFDYKPINANFDIGIYVLFLAKK
                     WSKRSNFPELLQKLFVLLQQMDQVRSGEYSIIDVLNRAHKDYPFFDFDREIKTKADLT
                     QWKAKKFVKQFDREMAWLFDFPTTLELKHSDDDEVPITISLNQFTASETESESIKNWW
                     DNFLLESKERTTEFESKEISQYQRIFMKDNNLFSDDTTGIIDED"
     gene            8721..9404
                     /locus_tag="PKH60_01985"
     CDS             8721..9404
                     /locus_tag="PKH60_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ16850.1"
                     /translation="MSSLASILLVESDKILSQQLMAILHEMSTVITQCFTIAEAFRVI
                     DLTKYDLVIVSREMSDGDGIEVAEYLNETALSTKILVVSKQKISLQEKILAYQAGASD
                     FLSKPIELLEFKYKISALLQLQKVMPNKILQYAAIKLNPTTGTLYLGDEMQTHLRKKE
                     SAILTCLLRHRPKIVTKSMIIDYVWGESNNMPTYSTIDVYIRRLRTHMGHSHHLIKTA
                     RGYGYYLAE"
     gene            complement(9405..10976)
                     /locus_tag="PKH60_01990"
     CDS             complement(9405..10976)
                     /locus_tag="PKH60_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16851.1"
                     /translation="MRKNTRLFQYLLFGFVAIVGYLAIHLVKLDALPVFADESIYIRW
                     AQLILDDARQYWIFPMNDGKTPLFIWTMVPMLRLVENQLLAGRSVSIFVGLFQAIVLG
                     IITWRMTKDKFSAVWVMLLTSTLPFWFFYQRMALMDSLLTFWLSVSVLLVLVMSEKVS
                     LFQKKIQTSLPYIGSLGLVLFLAIWTKLPAVLFLLALIPTSLIIKQKQSRHYLTILGL
                     TLTGISLSLVFFVITSILPAFSQLFSRGSDFLYSVPDFLAGGWKVVFFNSGLTTQSLV
                     YYLSPLVVLSPIIGLFFTKHRRTQSLLLLAATAFMLPMILLAKVYYPRYLLPVSIFFT
                     LSSGLIVDAALNKITIVNLKKRAVIALGLALYCANILAFAGQWNVQAMIDSNAVPMVA
                     VDRMQYLEEWSSGHGIVETVDLLQSVDKSQRIAVATEGYFGTLPDALLLYLHNQDVSN
                     LSIDGIGQPVWDIPQWFIEKTTAADERWLVVNSHRMRMTLPEDQLIAEYCRPNSAPCL
                     QIWNITEYSKTLENK"
     gene            complement(10989..12386)
                     /locus_tag="PKH60_01995"
     CDS             complement(10989..12386)
                     /locus_tag="PKH60_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16852.1"
                     /translation="MRNGIAVTPLLYLSLILFAFILIGYSIWSYGLTDPNLVLINWEQ
                     YWQFQQWAWQTLFHNRPLQTITYVVLISLLFVNYGVTTWLLQKLKLKMETFKQFLIAM
                     GIYLFVISPLLFSYNALSHDVFNYIFNARMVIEYQANPHSEVALGYSFDPWTRFMHNT
                     HTTAPYWYGWTAISLIPYLLGFGSFLITWLNFKLFALVSLLLYGMIMWFWKNHQKQTL
                     EHWQLGLVMLNPLVLVEIVGNSHNDFWMMVPALFAFLLLLNRPKQMHWLGLSLLSLIF
                     SASTKYATLVLLPVWLIVGKLFTINWGIPKATNAIASILVKFQNSLKTTFFNSFLNYV
                     VQLVPWMLSILLFLPLLTSRSRWFLPWYLLWSLSLVPLLVKSTVLNRELPKISLLPLA
                     SIKKQINNWRKRWVYLLVALSISSLIRYVPWLWYGEYSDLITWQEIVITWLGGFVFFC
                     VFCVTDYFTAKRSDS"
     gene            complement(12383..13141)
                     /locus_tag="PKH60_02000"
     CDS             complement(12383..13141)
                     /locus_tag="PKH60_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ16853.1"
                     /translation="MTYLSVIIPAYNEKENISKGLLDQVVEYLNQQSYTWEIIISDDG
                     STDGSVAALEQFAEKNKSVRVLRNKHAGKAQTVTSGMLNARGSWRLFTDFDQSTPISE
                     VKKLLERAEQGFDIVIGSREMVGAKRDKEPWYRHLMGRGFNLWVQMVAVPGVLDTQCG
                     FKLFSEKATTSLFPNLVVYGKQARFKDAFTGAFDVELLFLAIKNNFSIREVPIKWHHM
                     RTNRVSPIKDSLRMFRDLLKIKLASLTGKYGRQP"
     gene            complement(13178..14692)
                     /locus_tag="PKH60_02005"
     CDS             complement(13178..14692)
                     /locus_tag="PKH60_02005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNQ16854.1"
                     /translation="MVLLQRKLFWFLILLTLIGSWLRVYRLPETLQFLGDQGRDAIRV
                     ARMFKQGDLVFIGPVTSIGNMYLGPLYYYFMVPWLWLTYPSPLGPAYAVAVLSIMTIP
                     LLYILGKELVGKQAALIATICITFSSVVITHSRFSWNPNPAPLIGLLFMWALYRAWKN
                     HPRYWLLVAVFFSILIQLHYIALLSIFPALLVLILQIKENIRNKKNMVQLSLNICLAI
                     GIGFVFLSPLMVFDMRHGGTNYLALRQLLGGENSITTFESTNLLSKLWTIAEETHGRS
                     MQLLFDMYIGQQRTRNTIFLALTLLGVLWLLVKKKHFSKGELIVILSVFTAILGTSAY
                     SHNLYDHYVLYVVPGVLLLFGLLGSFIWQHGYIGRVLVMLLLSGFIYQNLPQLSFKPT
                     GTPLPLLAASAQVIAENIQPNEKYNIVLLSETKDYYGQNYRYFLDTIPNKTPLNPDHD
                     DLNDVSALVVINEEAVPIEAIDELPIFEIATFKDVNQKQIFKLPSGSEAYIWRK"
     gene            complement(14699..15877)
                     /locus_tag="PKH60_02010"
     CDS             complement(14699..15877)
                     /locus_tag="PKH60_02010"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ16855.1"
                     /translation="MKKAIIIIPTYNEKENIAVTVAAVFAATATVRSWKIEILVVDDT
                     SPDKTADEVKKIQKNNQAVHLLLNPKKMGLGGAYLAGMSHAFSKLDADVVFEFDADLS
                     HPPEKIPLFLKMLDDGKDMVLGTRYSNGGAIPADWGLHRKLMSMVGNLIIMLVFTNFS
                     IRDWTSGYRAITKQTYKKVVPFLQSERFFGYTFQIGFLYNAVKQGLSLGFIPYTFRDR
                     TKGKSKIGPEYMKNTLLYIFRVRIQEMLENRIFKFVIVGGIGAVVQFISLALFRRVMQ
                     YSFAYFFSAELAVASNFVLSNLWTFNDRTLSLAEIPKKFLTFNVASFGSVGIQTVLAT
                     LGKLLIGDTIPLFAIPLTQMILGNAFIFDTGFLFMIIGILIGMVWNFTAYNKFVWKQK
                     "
     gene            complement(15874..16848)
                     /gene="gmd"
                     /locus_tag="PKH60_02015"
     CDS             complement(15874..16848)
                     /gene="gmd"
                     /locus_tag="PKH60_02015"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNQ16856.1"
                     /translation="MQKKAFITGITGQDGSYLAELLLEKDYLVYGLTRRTSTQNFARI
                     EQIMHHPNLRLISGDLIDQGSLNTSLQSIQPDEVYNLAAQSFVQASWEQPVLTGEFTA
                     LGVTRVLEAIRLVCPKARFYQASSSEMFGKVREVPQNENTPFYPRSPYGVAKVYGHWI
                     TVNYRESYDLYAVSGILFNHESPRRGLEFVTRKIANGVARVKLGKQSHVELGNLEAKR
                     DWGYAKDYVVAMWLMLQQENPDDYVIATGETHSVKEFFQLACKVAGIENWESIIKHNP
                     IYDRPAEVDLLIGDASKAKKQLNWEPSLTFSQLVELMVQAELELEAKL"
     gene            complement(16866..17828)
                     /locus_tag="PKH60_02020"
     CDS             complement(16866..17828)
                     /locus_tag="PKH60_02020"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNQ16857.1"
                     /translation="MKKILITGGTGFAGTHLVEHLLNNPSSDSTEIHVTHFGNKLPAL
                     FEQFKPHQIHQVDLQDAIALDQLIKLVLPTEIYHLAAIAAVSSSYSNAARVIQNNSAI
                     QINLLESMIKFVPDARLLVIGSAEEYGMSLPGELPIREDHPFRPLSPYAVSKVTQDLL
                     AYSYFISRKLNILRVRPFNHTGEGQTVDFAIPSFASQIVEIELGKLTHLSVGNLDAKR
                     DISDVKDVVAAYQLVMQAGVVGEVYNIGSGHSVSMKDILNELIQYSSVPIAIETDTNK
                     LRPTDITEMVADITKITALGWQPKIGRDQTLLRVLHWWRTQHSI"
     gene            complement(17830..>19024)
                     /locus_tag="PKH60_02025"
     CDS             complement(17830..>19024)
                     /locus_tag="PKH60_02025"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNQ16858.1"
                     /translation="KQLKVGKVPFYEPHLEELLSETKKKQNLFFTSEYKDAVPQSDLI
                     IIAVGTPSSKSGGVDLTYVLSAIESMAPLLKQNTIIAIKSTVPPQAIDSVVDALRKNS
                     KVAYTIATLPEFLREGSAVSDTLHPARIVIGAEDDWTFSQLEILHQPFKAPIVRTKLA
                     SAQMAKYAANAYLATRITFINQIADLCEKNHADVQEVISAIGFDPRIRSHYWYPGLGY
                     GGSCFPKDVKELAYFSRQVGEAENLFNKIQELNDQRLTRLFTYFEQKVGGWKGKKVAI
                     LGLAFKPNTDDTREAPSTKLIPLLLESGAMVTAYDPKATLKGTTVVTASHQNHYAEKS
                     SIATCCENADVIIIVTEWPEIGSFNYGEVRTNQTQYLIDTRNQIQPEIVKNWGYQYLG
                     IGRGE"
BASE COUNT         5830 a         3936 c         3545 g         5613 t
ORIGIN      
        1 agcgcattga gattgatcaa aaataaaatt gtctacacca atcacacctt agttgctgct
       61 ggcaacatgt catatcccat ctcggcgtta actccattag ctgaaccata cgccaataaa
      121 atggaaatca gtacgcagca gttgctatct ctgggtagct ctggtaatcc tgattcattt
      181 tcagtaactt ctgcagccct caatgtttca catgtggcca gcggtgtaag ccaggttcac
      241 actgatctaa gtaaacaagt atggcccgat tacaactgga caaacataac caatggagtc
      301 catcggggca cttggcaaga ctctcaaatc aaattggcaa gtgaagactc aactaatttg
      361 tggcaaagac attatcaact aaaagaacaa ctccgtgact atgttcacgc aacaactggt
      421 tttggttatg atcatcatcg aatggtggtg tcttgggctc gcagaatagc ggggtacaaa
      481 caactcagtg cattattttc tgatatcgaa gctttacgaa gaatagctac acaacaaggt
      541 agggagattc agtttttggt ttctggaaaa gcccattttg gagactcatt cagtaaagat
      601 gtcctcaaag aagttattca acacttctcg tctgacttat ctggttatgc cctattcatt
      661 cccaactaca acattgaaat tgcgcaattc ttaacgaagg gttcagatgt ttggctcaat
      721 acgccaataa agggaaagga ggcgtcaggc acatcgggaa tgaaagcaat ttctaacggc
      781 gtccttcaga ttactgttca agacggctgg acaagcgaag tagattggaa ttctgtaggc
      841 tggacgctga atcctgacga tctcacctca agtctctatg aaatgttaca aaatgcccaa
      901 aacatgtatt ttcagaaaaa tgatcgtggt ttctctgaag aatgggttag tcgaatgaaa
      961 gcatccattg aattatttga tcgattttca accgaacgga tgctgcgtga atatagagaa
     1021 aaattgtata cagcatgaca aagaacgatc gctattttca gctcatgagt gagaaacacg
     1081 ccgagttgtt tgctgcattt gatccaattc accagcaatt tgaaaaaaat cgttctaatg
     1141 aggatgcatt tcataacgaa ggacaaaagg tggttgatgt tatacgtagt tgggaacgac
     1201 gactctgttc agggatggag cggggtcagc acgcccaata ttctcaacgg ttagcagaaa
     1261 aattttggac taaagttaaa gtccgatatc cactcattga gatggttggt ctgaaatcaa
     1321 aaactagttc tagctttggt acaccatgaa catcttcttc gaattgagtc tacttctcac
     1381 agttactgct atcattacat tggcagtacg caagcttcgg caaccgttag tagttggcta
     1441 tattctttct ggaatcattg ctggacccta tgttctcaat atcgtaagct cacagcatga
     1501 gctcgaactg ttttctaaag ttgggattgt ttttttactt tttattgttg ggctctacct
     1561 caaccccaaa attattcagg aagttggcaa ggtttctttg gttactgggc tgggtcaggt
     1621 gctatttacc tcactaatcg ggtttgctct cactttgtta ctcggtatag accgtttggc
     1681 cgctctgtat gtagctattg cactgacatt tagcagtacg atcatcattt taaaactaat
     1741 tgcagacaaa aaggatcttc agaaactcta cgcaaaaatt gctattggat tcctactcgt
     1801 tcaggatatc gtcgcaacct ttattttgat agccattacg gtagtaagtg ggcaatcaga
     1861 tatggggttg ggctcaacac ttttactcac tcttggcaag ggcatgattc tgttctctgt
     1921 gcttgggatg tttcaacgat atatactgcc caaagtcaca gaatacgcag cagaatctcc
     1981 agaactatta tttttattta gtcttgcatg gggaactatt ttagcgagtc tcttttctgc
     2041 gctcggcttt tcgattgaga tcggtgctct tattgctggc gtcacccttt cagtttcgcc
     2101 atactcggtt gaaatcgcct cgcgcctcaa accacttcgt gattttttta ttatcatctt
     2161 ttttgtactg ttgggttcac aactcattct cagcaactta gctcagttat tattccctgc
     2221 cataattctt tctctatttg tactagttgg caatcccatc atagtcatta ttttaatgaa
     2281 cactctagga tataacagaa aaacaagcta tcaggcgggg ttaacggtag cacaaataag
     2341 tgaattttcc ctaatcttag gggcacttgg cttacgcctg gggcacttat cgcaagaagt
     2401 actttcgctt atcactttgg ttggattaat cactatctct ggttcaacat atttaatttt
     2461 gtannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccgattg taaaaccaaa
     2581 gattattatt tctacagtgc ctgaattggc gacaaatatc ttgattgttc atgcgatgcg
     2641 agctaaaaat aaaaaggccg tcatcatccc aatcgctcaa acccatcaag atgctagtga
     2701 attatatgaa gctggggcct cctatgtgat gatgccacat catttgggtg cgcaacatat
     2761 gtctaaaatg attgcacgat atggcagtga tcatagtttg tacgaaacat taaaaaaacg
     2821 gcacgtctct gagttagccg caatgttcaa agcataagaa gaggaaattc cccctgagag
     2881 tgtcgctgaa actcagggga aatgatgttt attcttcact ggggaggacg tagattgagc
     2941 cctccaaaac ttgtgaggta ttgatactga aacttgattc agcactccca cccgtacttc
     3001 ttccctccat ctctcccagg ctgagtgcct gccaccctga gagctggaag ttaatgccac
     3061 taaatgtggc tctggcaaac acccgagtgt ttgttgcggt tctccccgtg aacaaaaaag
     3121 cgttcgcacc actgcgggca tactccagct ccatcgcctt gacaatcacg ggccgaagtt
     3181 ccttccgacc ctcgtaatcg tccgaggcaa agctgaagta tgacacttca tgtaaatcgc
     3241 ctgcgattgc ggctagcaga gtctcctctg ccaactctgc tccagccgca gtttcgtgtt
     3301 ggagctcagg cgcggtcggg agaataaacg cacccacatc attaaacggg cccgagacgg
     3361 cacggtcata ccactaggcg gggaaggagt aagtgacctt gctcccagtt tcgtggagag
     3421 cgctggttac atacacggtt tcccccacga atttgagtga ggttcgatac cggtgtcccg
     3481 tgtcaccagt atagtattcc aagccactgc caaaccaagc ggtaaaactg ctcggctgtt
     3541 ggcaggcttg ggatacattc tcaacaaggg taccatcgac atggtaccca atctctaggg
     3601 tggagtaaaa accacccata gaggtcaccg gtggccctgc aaagaactgg gtgaggtttc
     3661 gattagcctt acccataatc ttctggagcc tagcattcga ctcactaaac cgttcttctt
     3721 gaagaggata cagcaaaaag ctgttattgg gccttctttg cccctcgccg aacttcagtt
     3781 cggcttgaac aacttcatga tataggatgc tgtcatttct gccatacacg gtgtactcta
     3841 cgatcttttg gcaaggttcc ggttttcgtg ctggtggggc atcgttattg cagcccagca
     3901 acgaaaacat cacaattgtt aacaggagag cgaaaaaggg tttcatttca cacctcggtt
     3961 gcgacaccga tttagcctcc aacttctact gaagaacaaa tcggtgccgt aacgttggta
     4021 actatttgcg acactttttt aaagagcttg catagcaatt cattaattaa ccgcgattgg
     4081 tcaatcaata cacatgcaaa cacttatagt atttgatatt tacactcttt aatcaatatt
     4141 ccacacaaac agtcaggtgt taaaataata actatatgaa aaacttgcaa gcgttttatt
     4201 tagtacttgt tgcttcatat tttatggttg gttgcaccat ccaaccaaat gatctaaaaa
     4261 ctagcgaaat acaggatccc actccaattc caacaactac tgcaccagaa ccaacggctt
     4321 caacagaaat gtatcttggt caacaaacaa atccctccga agcgtatgaa aaactctgtg
     4381 cctgggtaga caaatcagat atcatcatcg agctctcttt cgcagacgga gtagttaaag
     4441 atacttcgta tgaggcaggt tttgcgttta gctcaattga aagaactgct cgactagcgc
     4501 gcgaaggctg taattaccga acgggattta aattttcaga attagctaat aaaggagaag
     4561 ttcaggtcgc tgtttgggga tataatgaca atcgcaagcg tcagctacta aacgagccaa
     4621 ttaatctcac atttgactct gaaggtaaac caaacatctc cctacctatt caagttacag
     4681 ttttagacta gcgaaataat catttcttca aaatcgaagt gcaatacatg catcgcttcg
     4741 cattaattgg aattttactc aaacactctg gacatacttt gtccgtagga tctttttttt
     4801 cttctttttt cattgcggcc ataattttat tcatcggaac aatcacaacg aaataaatta
     4861 ctccggcaat cataacaaaa gagattaccg aattaagaaa atttccgatc ataaatttac
     4921 tattgttcac agtaaaatat atatttgaaa aatctggctg accaccaatg gcgccaatta
     4981 ttggagtgac tatgtctttc accaaagaag taacgatgga accaaaggct ccgccgataa
     5041 caacggcgac tgctaaatcc acaacattac ctctaaataa ataatctctg aaacctttta
     5101 agacgctcat ggcttcctct ctttattact aattatacgt ttaagacagc cagtacggtt
     5161 gtcgaatact ctacttctac tagaatttta tcagcaattt tctgtttatt aagccttctg
     5221 attatttgat cctcttcatt aatttcccaa agcgagaaac cagattcttc tagtatttga
     5281 agatagtttc gccaatcaca accagcgagc tttaagccat acggccataa ctcactgaga
     5341 atcactttaa ttcttttctt ctgaaagagt tggtttgcgc ccactatggc aaaatgctcg
     5401 tatccttgaa tatcaatttt tagtaaatca atatcatgag gatactcact aaaataatca
     5461 tctagagaaa ccaccctcac gacttccgtt tctctgttgt caccactatc aaatgtgcga
     5521 tgatctccca gattatccgg gctgatatac aaagatattt caccactttt ttgagctata
     5581 gctacttttt cacaggtaat gttttctaat ttattttcct tcaccgtttt tgtaagtgat
     5641 tgaaaattaa cttcatctgg ttcaaacaca aatatttctg ctgttgactt ggagcatgct
     5701 gcccccagca gagaaaaata tccaatattc cctccaacat caactatagt ccttgctttt
     5761 tttgcgattt gaataaattg tttggaaaca aatggttccc aggtacctgt ttttgcaagt
     5821 gtagcagaaa caacagtgtc cattggattg atatacaatt tataaccctc aatttgtaca
     5881 taattttttt tgaatatcgc aacaaatctt ctagtaaacc gatatttttg taatcgagaa
     5941 aaaataaaat aacccaatcc cttgtgaatc aagtcgtcaa tatatatttt aattctggat
     6001 ttggtcatat aatcttgagt cattgtatca aatgatctaa aacaaatgtt cttctaatat
     6061 tatttggctg tttcttaaga tacaatttat tacctaatga ttaacgaaaa ttcaaatata
     6121 caagaagata aggctgacaa tgatttggag cgcaatttaa gattaaatcg aactcatcgt
     6181 ttgttagcag ctgcaaagca ggagttggta gatgcgaaaa taagactttc tttagttggt
     6241 gctaatgcat caggcttaaa caaaatacat acttctcttg aaagacaggg agcaaaaagc
     6301 gtagttgcac ttcttgaata tcggatttta tatttacagc aagagttggt atttattaga
     6361 gctaaaaatc aggctgatat tgagtataga ctactcttac gcagagagtt ttctgctaaa
     6421 ctatcacacc gatttggcga aactccactt ttgtttcatg gagcaaactt aattactact
     6481 ctagagataa taaaatcgaa atcaatttca tcaacggcag agaggggctt gggtcaagtt
     6541 agctatgatg ttgatggctc cacctctgta agtccagctg atgctgttca agttagctta
     6601 gttgactaca ccggtattaa agatctgttt attccaatgg gatgcttatt tgtaattaaa
     6661 aatgaatctt ccacaaaaaa tgtatctgca attcaaagtt tttctctttt taaggatggt
     6721 cagactaacc catgcttaat tgcaatctta gcatctagtg aatctttacc gataataagg
     6781 tccaaattgg ttgaatgtgg atatccagca gaaattgtcc aagaatactt tgctttttta
     6841 gaaacccaaa gagaatacta acgcttcttg atgcctcgct aagatttacg actcgctcac
     6901 tttattcgct tacctgcact aggcgggtgt ttcgctaccg ctccgcactc gccgtcgcga
     6961 accagaagct ccaaatctac caacgaagtc ataaaacaaa tcagcggttg ctgatttgtt
     7021 ttatgatgcc tcgctaggat tcgaacctag attctgtggt tcagagccac atgtcctgcc
     7081 cttagacgac aaggcaaata tcggtgagcg actgtattgt aacaaatatc atccagacat
     7141 aaaacatcag tagtttatct ttatgttagg atagcaacac aatgttacaa actgaaatac
     7201 tccaatccga tcgcgaggtc tccagattgt atactgccgg attatctgag gctctggata
     7261 ctaaccctcc agtttcttgg tggcgttcaa tcgctaaaaa acttatttat tttgaacgac
     7321 atcttagtga gattcaatcc gcacctattc gaaaccacat acaagaaaaa atcgctgagc
     7381 ttaaaacgat attgagaaat tctcaatatt taccaatgaa atcgccgctc gacatagttg
     7441 aatatacgga aaaagtcatt atgtcacttt ccatggaaat ggctctcatt ttcgaagagg
     7501 agcgggagcc cattactgag catattattt ccttggatga cttagttcat acgtatcccc
     7561 atagattacg aaaaaatgaa tcaatgctga atgacacacc aattattgaa cttgaaattc
     7621 gtcatcccga tccagagatt gagcaatggt actcgattcc tattccaaat caaaaaatga
     7681 tttggcacaa gggtggacca gccagagctc tgttaaattt gatcgctgat gcacccctat
     7741 cgatgcaaat tgccgaattc ccctggaatg actttgatgt tatcacggcc ggagatgacg
     7801 tttacgacac ggcgattagt attggagtag atcccgatgg agttgagtac atgggagaaa
     7861 atgcgctcaa tttctctcgt tttgcttatg gccgtgacac cgatcaaaat caatgttgtt
     7921 tgggttcaga tggattacac tactcaactg cagctcttca ggcagctcaa accggtcaca
     7981 ttaatattgt tggtaagtac attgctaata aagccatata tggaattgac tcaatgtatg
     8041 ccgaaggaat cagtctgata aaacccaggg gattaatgcg gttagtaaag aatttggtag
     8101 agggaaaagc actttccttt gactacaaac cgattaatgc aaattttgat attggtattt
     8161 atgtgctttt tctagcaaag aagtggtcaa aaagatcaaa cttcccagaa ctacttcaaa
     8221 aactttttgt cttgttacaa caaatggacc aggtacggag cggagaatac tcaattatcg
     8281 acgttttgaa tcgagcccat aaagattacc cgttttttga ttttgataga gaaattaaaa
     8341 cgaaagcaga tcttactcaa tggaaagcaa aaaagtttgt caagcagttt gacagagaaa
     8401 tggcctggtt atttgacttt cctacaaccc tagaattaaa acactccgat gatgacgaag
     8461 taccaattac catttctctc aatcagttta ccgcttcaga aactgagtcg gaatcaatca
     8521 aaaactggtg ggataatttc ttacttgagt ctaaggaaag gactactgaa tttgaatcaa
     8581 aagaaatttc acagtaccaa cggattttta tgaaagataa caatcttttc tctgatgaca
     8641 ctactggaat aattgacgag gattaatttg cacacttact atgcattttg ttattttatg
     8701 gttgttttat aattcagcgc atgtcgtcgc ttgcctcaat tctacttgtg gaatctgaca
     8761 agattctctc tcaacaactt atggcaattc ttcacgaaat gagtacagta atcactcagt
     8821 gttttaccat cgcggaggct tttcgggtaa ttgatttaac aaaatatgat ctagtcattg
     8881 tttcaagaga aatgtcagac ggcgatggca tagaggtcgc ggaataccta aacgaaaccg
     8941 cgctgagtac aaagatctta gtcgtatcta aacagaaaat atctcttcaa gaaaagatcc
     9001 tagcttatca agctggagct agtgattttt tatctaagcc aatcgagtta cttgaattta
     9061 aatataagat ttctgcttta ctccaactgc aaaaggttat gccaaacaaa atattgcaat
     9121 atgcagcaat taaattgaac ccgactactg gaacgctcta tttgggggat gaaatgcaaa
     9181 cccaccttcg aaaaaaagaa tcagcaattt taacttgttt gctgagacat agacccaaaa
     9241 ttgtgactaa atcaatgatt attgactatg tttggggtga aagcaacaac atgccaactt
     9301 attcaacaat tgatgtctat attcgtcgat tacgtacaca catgggtcac tctcatcatt
     9361 taattaaaac cgcaagagga tatggctact atctggctga atagctattt gttttcgagt
     9421 gtcttgctgt actcggtaat attccaaatt tgtaaacagg gagctgagtt tggtcgacaa
     9481 tattcagcta taagctgatc ttctggcaat gtcattctca ttctatgact attgactact
     9541 aaccaacgct cgtctgcagc tgttgttttc tcaataaacc actgtggaat gtcccaaaca
     9601 ggctgaccga tgccatcgat tgataagttt gatacatctt gattatgcaa atataaaagc
     9661 aatgcatcgg gtagtgttcc aaaataaccc tctgttgcaa cggcgattct ttgcgatttg
     9721 tccaccgatt gaagcaaatc gacagtttct acgataccat gccccgatga ccactcttcc
     9781 aaatactgca tcctatcaac tgccaccatt ggtacagcat tgctgtcgat cattgcctgt
     9841 acattccatt gaccagcaaa agccagtatg ttggcacaat ataaagctaa tcccaatgca
     9901 atgacggctc gctttttcaa attaacaata gtaattttgt tcaatgctgc gtcaactatt
     9961 aaaccagaac tcaaggtgaa aaaaatacta actggtaaca agtatcttgg ataatagact
    10021 ttggccaata agatcattgg caacataaat gcagttgcgg caagtaataa cagcgactgc
    10081 gtacgacgat gcttagtaaa gaaaagtcca atgattggtg aaagaactac caaaggactt
    10141 aagtaatata ctagagattg cgtagttaag cctgagttaa aaaatactac tttccatccg
    10201 ccggctagaa aatcaggtac tgaatataaa aaatcgcttc cacgactaaa taattgtgaa
    10261 aatgcaggta agattgaggt tatcacaaag aataccaacg agagcgaaat gcctgttaac
    10321 gttaatccaa gaatagttaa atagtgacga gactgttttt gcttgattat caagctggtt
    10381 ggaatgagcg ccaataaaaa taatactgca ggtagttttg tccatatagc tagaaacagt
    10441 actaacccca aacttccaat atatggcaaa ctagtctgaa tttttttttg aaacagcgag
    10501 actttctctg acattaccaa cacaagtaaa accgatacgc ttaaccaaaa tgtcagaagc
    10561 gaatccatta gagccattcg ttgatagaag aaccaaaatg gtagagtcga cgtaagcagc
    10621 atcacccaaa cagcactgaa tttatccttc gtcatcctcc aagtgattat gccgagtaca
    10681 attgcttgaa acaagccaac aaaaattgat acagatctcc cagcaagtag ttgattctct
    10741 acgagtctca acatgggcac catggtccaa atgaaaagtg gagttttgcc atcgttcatt
    10801 ggaaatatcc aatattgtct tgcatcatcc agaatcagtt gcgcccaacg aatgtaaata
    10861 gactcatctg caaatactgg aagcgcatca agttttacca gatgaatagc caaatatcca
    10921 acaatagcta caaaaccaaa aagtaaatat tggaacagcc tggtattttt tctcacctct
    10981 ctattgtatc aactatctga gcgtttcgct gtgaaataat ctgtaacaca aaatacacaa
    11041 aaaaatacga aacctcccaa ccaagttatc acaatttctt gccatgtgat gagatcggaa
    11101 tattctccgt accataacca aggtacgtac cgaatgagcg aactgattga taatgctact
    11161 aataaatata cccaacgttt tctccaatta tttatctgct ttttgattga agctagcggc
    11221 agcaatgata ttttaggtaa ttctcgattt aaaaccgtgc ttttcaccaa taatggtacc
    11281 aagctcagac tccatagtaa ataccacggt aagaaccagc gtgaccgact tgtgagaagc
    11341 gggagaaaca acagtatgct cagcatccac ggtacaagct gtactacata atttagaaag
    11401 gagttaaaga aagttgtttt aaggctattt tgaaatttaa ccaatattga tgctattgca
    11461 ttagttgcct tcggaattcc ccagttgatt gtaaataatt tgccaacaat cagccatact
    11521 ggtaacaata ccaacgttgc atattttgta ctagcagaga atattaaact caataaagag
    11581 agtcccagcc aatgcatctg tttcggtcta ttaagcaata gtaagaaagc gaataaggct
    11641 ggtaccatca tccaaaagtc attgtgagaa ttacccacaa tttctactag taccaatgga
    11701 ttcagcataa caagccccaa ctgccaatgc tctaaggttt gtttttgatg atttttccag
    11761 aaccacataa tcatgccgta caagagcagg cttactaaag caaatagttt aaagttaagc
    11821 caagtgatta aaaatgaacc aaaacccaac aggtatggaa ttagagagat tgcagtccaa
    11881 ccataccaat acggagcggt cgtatgtgta ttatgcatga atctcgtcca cggatcgaaa
    11941 gaatatccaa gcgctacttc actatgtgga tttgcttgat actcgattac catacgagca
    12001 ttaaaaatat agttaaaaac atcatgtgaa agagcgttgt aggaaaataa tagtggagaa
    12061 ataacgaata aatagatgcc catggcgatt agaaactgct tgaatgtttc cattttgagc
    12121 tttaattttt ggagtaacca cgtggttacc ccatagttaa caaacaataa actaatcaga
    12181 acaacgtacg taattgtctg cagtggccga ttgtgaaaga gcgtttgcca agcccactgt
    12241 tgaaactgcc aatactgttc ccagttaatc aaaaccaagt ttggatctgt caatccatat
    12301 gaccaaattg agtacccgat caaaataaat gcaaatagta tcagactgag atagagtaag
    12361 ggggttactg caataccatt tctcatggtt gcctaccata tttgcccgtc agggaggcta
    12421 attttatttt taacagatct ctaaacatac ggaggctatc tttgattgga ctcacgcggt
    12481 tggtcctcat atgatgccac ttaattggaa cttcgcgaat tgaaaaattg tttttaatcg
    12541 ctaaaaacaa taattcaaca tcaaaggcac cggtaaaagc gtctttaaat ctagcttgct
    12601 taccataaac aactaaattg ggaaataaac tagtagtggc cttttctgaa aatagtttaa
    12661 acccacactg agtatcaagt actcctggaa ctgccaccat ttgtacccaa agattaaacc
    12721 cacgtcccat caaatggcga taccagggtt ccttatcccg ttttgctccc accatttcac
    12781 gagaaccaat gacaatatca aatccttgct cggctctttc taataatttt tttacttcgg
    12841 agattggcgt ggattgatca aaatcagtaa ataatctcca actaccccta gcgtttaaca
    12901 ttcccgaagt gactgtttgg gccttgccag cgtgcttatt gcgcaatact ctcacacttt
    12961 tattcttttc agcaaattgt tctaaagccg caacactacc atcggttgat ccatcatcag
    13021 aaatgattat ttcccatgta tatgattgtt ggtttagata ttcaacaact tggtccagta
    13081 gccctttgga gatattctct ttttcattgt aggctggaat tatcactgat aaatatgtca
    13141 tagacgaaga ttgtacacta aatgacacca aatattctta ttttctccat atgtatgctt
    13201 ctgagccaga tgggagtttg aagatctgct tttggttaac atctttgaag gtggctattt
    13261 caaaaattgg caattcatca attgcctcaa ttggcaccgc ttcttcgtta attactacca
    13321 gagcgcttac atcattgaga tcatcatggt ctggattaag cggagtttta tttggaattg
    13381 tatctaaaaa gtaacggtaa ttctgaccat aataatcttt tgtttctgaa agtaaaacga
    13441 tgttgtattt ttcatttggc tgaatatttt ctgcaattac ttgagcagat gcagccaaga
    13501 gtggcagggg tgtaccggtg ggtttaaatg aaagttgtgg tagattttga tatatgaatc
    13561 cactcagaag aagcattact aatactcttc caatatatcc atgctgccat ataaaactac
    13621 ccaacaatcc aaacaagagc aatacacccg gcacaacata taaaacataa tggtcgtata
    13681 ggttatgaga atacgcgctg gtaccaagaa ttgcggtgaa tacagacaat attacaatta
    13741 attctccctt tgaaaagtgc tttttcttaa caagtaacca taatacacca agtaaagtta
    13801 gtgctaagaa gattgtattt ctggttcttt gctgaccaat atacatatca aagagcaact
    13861 gcattgatct accatgagtt tcctccgcaa tggtccacaa cttgctcaaa agatttgttg
    13921 attcaaaagt agtgatggag ttttctccac ctaggagttg tcgtagagct aagtaattag
    13981 tgccaccgtg cctcatatca aaaaccatca gaggactcaa aaacacgaaa cctataccaa
    14041 tggctagaca aatatttaaa cttaattgca ccatgttttt tttgttgcgg atgttttctt
    14101 taatttgcaa aatcagtact aacaaagcgg ggaatatact caataaagcg atgtaatgga
    14161 gttgaatcag aattgaaaag aaaactgcta cgagcaacca atagcgagga tggtttttcc
    14221 atgcgcgata cagtgcccac ataaacaata atccaattag cggagctggg ttgggattcc
    14281 aactaaaacg actgtgtgtg atgactacgg aactgaaagt aatacagata gtagcgatga
    14341 gagcagcttg ttttccaacc aattctttgc caagaatata cagaagtgga atcgtcatga
    14401 tagataaaac ggctacggca tacgcagggc caagtggact tggataggtc aaccaaagcc
    14461 aaggaaccat aaaatagtaa tacaatggac caagatacat attcccgatg ctagtaaccg
    14521 gaccaataaa aactaaatca ccttgcttaa acattctggc tactcttatt gcatctctcc
    14581 cctgatctcc caaaaactgt aaagtttctg gtaatcgata taccctcaac cacgaaccga
    14641 taagtgttaa taaaataaga aaccagaata gtttacgctg taataaaacc atacagagtt
    14701 atttttgttt ccaaacaaat ttgttatatg cagtaaaatt ccaaaccata ccgatgagga
    14761 taccaataat catgaataag aagcccgtat caaaaataaa tgcattgccc aaaatcatct
    14821 gcgttagagg aattgcaaat aaggggattg tatcgccaat aagaagtttg cccaacgtgg
    14881 cgagcactgt ttggattccc accgagccaa agctggcaac attgaacgta agaaattttt
    14941 tagggatttc tgccagagaa agcgttctgt cgttaaatgt ccaaaggttt gataacacaa
    15001 aattagaagc tactgccaat tcagcagaaa aaaagtaagc gaagctgtat tgcattaccc
    15061 tgcgaaataa agctagcgaa ataaattgca caaccgcccc tatcccacca actatcacaa
    15121 atttaaaaat acgattttca agcatttctt gaatcctcac tcgaaaaata tacaacagag
    15181 tgtttttcat atattccgga ccaatttttg actttccttt cgttctatcc ctaaaagtat
    15241 agggaataaa tccgagtgaa agaccctgtt taaccgcatt gtatagaaat ccgatctgga
    15301 atgtatagcc aaaaaatctt tcagattgta aaaaaggaac aactttttta taggtttgtt
    15361 ttgtaattgc tcgataccca gaagtccaat ctcgaataga aaagttggta aaaaccaaca
    15421 taatgattaa gttacccacc atgctcataa gttttcgatg taaaccccaa tcagccggaa
    15481 ttgctcctcc attactatat cgagtgccta acaccatgtc tttaccatca tctagcattt
    15541 ttagaaacaa gggtattttc tctggtggat gagacaaatc agcatcaaac tcaaatacca
    15601 catctgcgtc caattttgag aaagcatggc tcatcccagc taagtaggct cctcccagcc
    15661 ccatcttttt gggattgagt aataaatgaa cggcttgatt atttttttgt atttttttaa
    15721 cttcatcagc ggttttatct ggcgaagtgt catcgacaac tagtatttca attttccagg
    15781 atctaacagt agctgtggct gcgaagacag cggcaactgt caccgctatg ttttcctttt
    15841 cattatatgt cggaataata ataatggctt ttttcatagt ttggcttcta attctaattc
    15901 tgcttgaacc attagttcaa ccaactgact aaatgtcaga cttggttccc aattgagctg
    15961 tttttttgct ttacttgcat ctccaattaa gagatccact tccgcagggc gatcatatat
    16021 aggattatgt tttatgattg actcccagtt ttctatgcca gctactttac aagcaagctg
    16081 aaaaaattct ttgacagaat gtgtttcgcc agttgcaatt acataatcat ccgggttttc
    16141 ttgctgcaac attagccaca tggcaaccac atagtctttt gcataacccc aatcgcgttt
    16201 tgcctctaga ttacccagtt caacatgtga ttgtttaccc aacttgaccc gagcaactcc
    16261 attagcaatt tttctggtta caaactccaa acccctcctt ggcgattcat gattgaacaa
    16321 aatgccagaa actgcgtaca aatcatacga ctcccgataa ttgacagtaa tccagtgacc
    16381 atagactttt gccactccat agggagaacg aggataaaat ggtgtatttt cgttttgagg
    16441 aacttcacga accttaccga acatttccga tgaactggct tgatagaagc gcgcttttgg
    16501 gcagaccagt ctgattgctt cgagaactct ggtaactccc aaggcagtaa attcacctgt
    16561 tagtacgggt tgctcccaac ttgcttgaac aaaagattgg gcggccaagt tgtacacttc
    16621 atctggctga atcgactgca aggaagtatt caaagatccc tggtctatca aatctccaga
    16681 aattaaacgc agattgggat gatgcattat ctgttctatg cgcgcaaagt tttgcgtact
    16741 ggttctccga gtgagaccat agaccagata atccttttcc aaaagcagtt cagccaaata
    16801 agagccgtcc tgacctgtta tgccagttat aaatgctttt ttttgcatat caatcaatac
    16861 cctttctaaa tactatgttg tgttctccac cagtgtaaca ctcgcagaag agtttggtca
    16921 cgaccgatct ttggttgcca gcctaaggca gtaattttag taatgtcggc aaccatttct
    16981 gtaatatcag ttggtcgcaa tttatttgta tctgtctcaa tggctatggg aactgaggaa
    17041 tattgtatta attcattaag aatatccttc atgctcactg aatggccgct gccgatgtta
    17101 tatacttccc cgacaacacc tgcctgcatt actaattggt acgctgccac cacatccttg
    17161 acatcgctaa tatctctttt ggcatcaaga ttgcccactg agagatgggt cagtttacca
    17221 agttcaattt caactatttg cgatgcaaat gaaggaatcg caaaatcgac agtttgtcct
    17281 tcgccagtgt gattaaatgg tctcactctg agaatattta gtttcctgct gataaagtaa
    17341 ctatatgcca acaaatcctg agtaactttt gaaacagcat aggggctcaa gggacgaaat
    17401 ggatgatcct ctctaattgg tagctcacct ggaagtgaca tcccatattc ctctgcagaa
    17461 ccaataacta atagtcttgc atccggcaca aactttatca ttgattctaa tagattaatt
    17521 tggatagctg aattgttctg gattacccgc gctgcattag agtaactgga tgatactgca
    17581 gctatagccg ccaaatgata aatctctgtg ggcaaaacta acttgattag ttgatcaaga
    17641 gcaatggcat cttgcaaatc aacttgatga atctggtgag gcttgaactg ctcaaataac
    17701 gctggaagct tattcccaaa atgagtcacg tgaatttcag ttgaatctga tgaaggattg
    17761 tttaataaat gttcgactag atgtgtgccc gcaaaacctg ttccaccagt aattaggatt
    17821 tttttcatct tattcccctc ttccaatacc caaatactga tagccccagt tttttactat
    17881 ctctggttgg atttgatttc tcgtgtctat caaatattgc gtttgatttg ttctaacctc
    17941 tccgtagtta aaactgccaa tttcaggcca ttctgtaaca ataatgatga catctgcgtt
    18001 ctcgcaacat gttgcaatac tagatttctc agcatagtga ttttgatgac ttgctgtaac
    18061 tacagtagta cctttcaaag tagcctttgg atcatacgct gtcaccatgg caccggattc
    18121 cagcaataat gggattaatt ttgtgctcgg ggcttcgcgt gtgtcgtctg tattgggctt
    18181 gaaagccaaa ccgagaatag ctactttttt acctttccat cctccaacct tttgctcaaa
    18241 atatgtaaat aatcgagtga gccgctggtc gtttaattct tgaattttat taaataggtt
    18301 ttctgcttca cctacttggc gagaaaaata cgcaagttct tttacatcct tggggaagca
    18361 tgagcctcca tagcccaatc ctggatacca gtaatgcgaa cgaatacgtg ggtcaaaacc
    18421 aatggcggaa attacctcct gaacgtctgc gtgatttttt tcgcataagt ctgcaatttg
    18481 attaatgaaa gtaattctag tagccaaata tgcgtttgcc gcatatttgg ccatttgagc
    18541 cgaagctagc ttagttctca caatcggagc cttaaacggc tgatgcagta tctcaagctg
    18601 actaaatgtc caatcatctt ctgcgccaat aactatcctc gctgggtgca gcgtatcact
    18661 gactgcgctc ccttccctta aaaattctgg caaagtggcg attgtgtagg caactttaga
    18721 attttttctc aacgcatcta ccacactatc aattgcctgg ggcggcacag tagattttat
    18781 tgcgataata gtattttgtt tgagtaaagg agccatcgat tcgatagctg acaaaacgta
    18841 ggttaaatca acaccacctg attttgatga aggagttccc accgcaatta taatcaaatc
    18901 ggactgagga actgcatctt tatattcaga agtgaaaaat aaattttgtt tcttttttgt
    18961 ttcggaaagc aattcttcta gatgtggttc ataaaatgga acttttccta cttttaattg
    19021 tttt
//
LOCUS       DAOQZP010000018        18593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_3362_length_18593_cov_5.543802, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000018 DAOQZP010000000
VERSION     DAOQZP010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 18593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18593
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3362_length_18593_cov_5.543802"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..148)
                     /locus_tag="PKH60_02030"
     tRNA            complement(75..148)
                     /locus_tag="PKH60_02030"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Ala,seq:ggc)
     gene            complement(359..1297)
                     /locus_tag="PKH60_02035"
     CDS             complement(359..1297)
                     /locus_tag="PKH60_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNQ16859.1"
                     /translation="MSLPTEPFLLLTQYLEHLEIERNVSRLTIRNYSLYLRRFCTWLK
                     DLEITDVRQIDKEVVRKYRIYLARYEDDQGNLLSKRTQSYYVIGLRSWLKWLVKNDVP
                     VLQPEKIDLPKAEARLMKFLPIESIERLLGQPDLSSQSGLRDKALLEVLFSTGLRVSE
                     LVNLNRDQVSLASQEFGVIGKGRRPRVVFLSDRAKQWLERWLNTRRDNWDPVFIRFSG
                     KQPDILSDGEEMRLTARSVQRMVDAYCRKARLPIRVSPHGIRHSFATDLLSNGASLRD
                     VQEMLGHKDIGTTQIYTHITRPQLKQAHHKFHSDLK"
     gene            complement(2534..3301)
                     /locus_tag="PKH60_02040"
     CDS             complement(2534..3301)
                     /locus_tag="PKH60_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16860.1"
                     /translation="MRRREKFIISSLVLSFALIGVLLIPTAWRVWSLLLFGGVTYSLA
                     AWALSEDLQKNEYLTILPLPTLYALAIASFYFVLPASMPKRVLFIVLFGVGMYALLLT
                     GNIFSVAKGRTIQLLYAAQTIALFFSLLISFLITNTLFTFNLPFAVTAGLVAAFHFPL
                     IAMSIWSVQLRSKLDRTDWALTGLLTLAVFELSIALSFIPYAVWAIGLLIMSLLYIGL
                     GVFQSYLKGRLFIKTIREYSLVTGVSILLFLLLFPGK"
     gene            complement(3298..3891)
                     /locus_tag="PKH60_02045"
     CDS             complement(3298..3891)
                     /locus_tag="PKH60_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNQ16861.1"
                     /translation="MNTTERIDFMAGHSKWHNIKNRKGAVDSQRSKIFGDLAKHIRKA
                     VKEGGSGDPQFNPTLRTFLDKARMANMPREKIQKAIDKGSGKSASGVTIQEVLYEAFG
                     PYGEAYLVQAVTDNPNRSSSEMKFIFSRHGGSISGPGSARYLFERTDGAEYRASMPLE
                     LSSEQLEAVQTLISTLLDHEDVEDVYCTAKIDLEQNS"
     gene            3987..4718
                     /locus_tag="PKH60_02050"
     CDS             3987..4718
                     /locus_tag="PKH60_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16862.1"
                     /translation="MSAATGSLKQLAVLAAKQAEWQKAIELNQAILEKEPTDVGALNR
                     LGVSHLQLNQVAEAKKAFKVAIGLDKFNTIAKKNLDKLNQSDSTNQTLTFSKQQFIEE
                     SGKTKVIELHRLAGKQVLETLSVGTKCQFMCKKRFISIETNGTYIGALPEDISFRLSK
                     LIQNGNQYECYIRSVSSSTCSVYVRELFRSEDNLDVSSFPINRSAHFAGDDIDDSLLK
                     EDAALEYAGFENEEAEETEEPADLE"
     gene            complement(4710..5579)
                     /locus_tag="PKH60_02055"
     CDS             complement(4710..5579)
                     /locus_tag="PKH60_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HNQ16863.1"
                     /translation="MPARKKKHSVRLRSTKPRKRWWSLFLFLALVLIALWVTLSLSIN
                     AILRWVPKGFQNQVIAITDSPKAGQSGSILIAQLDPEKTYSKLIIVPSSSMAGELHKP
                     WEVAAATGVLVNQIILLENTNFADNHSTKRTIQTNLTNLLKLGKFKEAWRLLPIWQLL
                     QDEGVQIIENPEHAASELVILGRLGETCPIGIANTTSISGVAGKYADLVAKQGGLVVR
                     VTNREVTTDETTIFIDDQLTTECNLVASLLQASLPGEVKMEVISELFSSDRVGVLVQL
                     GSSSAEQLQKISL"
     gene            complement(5606..6778)
                     /gene="tgt"
                     /locus_tag="PKH60_02060"
     CDS             complement(5606..6778)
                     /gene="tgt"
                     /locus_tag="PKH60_02060"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNQ16864.1"
                     /translation="MPNSFRFSLITSRGLARAGSITTPHGQIKTPIFMPVGTRASVRS
                     LDSLDLEQLEAQIILGNTYHLYLKPGTKIIEAAGGLHQFMRWDHPMLTDSGGYQVFSL
                     GEQQRAKGLASTVKISEEGVIFTSHLDGSKHVMTPETSIQIQQSLGADIIMVFDECLP
                     DSSSEKMMNDSVERTTRWAQRCKIEWESAKKQSLYGKYQALFGIVQGSTSLVLREKSL
                     RSLLDIGFDGYALGGETVGYDLAGTQHVMASIGKLLPEQAPRYAMGMGRDPQDVIEAI
                     LMGFDMFDCVGPTRLARNGALFVGHLDTTTKTPVFKSEFPKGRLAISNQRFATDHNVL
                     QADCDCSTCRGGYTRAYLHHLYNVAELSFYRLASIHNLRVMVRLATEMRDWIVQST"
     gene            6800..7870
                     /gene="queA"
                     /locus_tag="PKH60_02065"
     CDS             6800..7870
                     /gene="queA"
                     /locus_tag="PKH60_02065"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNQ16865.1"
                     /translation="MLSINQFDYTLPPSLISHAPLTKRTASRLLSLDRTSKKITHHHF
                     SDLAALLQPGDVLIRNETKVIPARLFGQRKSGKKIELLLISPVDDSGFKWKCLTKPGL
                     NQNEKILFNSEIEAMNTSPKESYVKEVEFGCSKTELRAFIVKNGHTPLPPYISNQSNE
                     LLLRKQYQTTYAKHEGSVAAPTAGLHFTKELDKELALRDISILPITLHVGLGTFAPLK
                     ETVFAEKQLHAEWFSVSDMTAQQINQAKQEKRRIIAVGTTSARTLESCTNSKGLIEPK
                     TSETALFIFPPYKFRSVDGLITNFHLPKSSLLTLVSAFVSAPNTPENFTSFNKSLIGK
                     SYQTAISEQYRFYSFGDAMLIT"
     gene            complement(7867..8325)
                     /gene="tsf"
                     /locus_tag="PKH60_02070"
     CDS             complement(7867..8325)
                     /gene="tsf"
                     /locus_tag="PKH60_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNQ16866.1"
                     /translation="MSINAADIKKLREETGAGVLDCQRALAETEGNYQQAMALVKERG
                     LAKADKKSDRETKEGAIFSYVHSGGRVAALVELVCETDFVARNAEFQALGKNIAMQVA
                     AMNPENVEELLEQEYIRDGGATISYLVKELSGKIGEKIAVSKFVRYELGV"
     gene            complement(8328..9314)
                     /gene="rpsB"
                     /locus_tag="PKH60_02075"
     CDS             complement(8328..9314)
                     /gene="rpsB"
                     /locus_tag="PKH60_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNQ16867.1"
                     /translation="MLDVTSLPDKAPDFDLQELFGAGMHYGHHKSKWQPRMTQYIHSE
                     KNGVHIIDLAKTAQQMRLAYNLLYQLGAQKKSVIIIGTKRQARELVKQAAETSGMMHI
                     TARWLGGLMTNWSQVKKSLKDMVDTEAKLAEGSLKGRTKYELVKIEKEVSRAKRFFDG
                     IRSMNDLPAALIIIDPGKEKIVVAEAEKVGVPVIALVDTNTNPDLVDIAVPGNDDAIK
                     SIECFLQHMSKAYVAGKAGGVEPSKINVAETVKAAVVTTPEEKQTVEPIVAKEVTKPV
                     AKQVVKPAVEQKVTAKTVKASSPKVIKKPVVKEVKKTTKSAKVTKTSAKKKE"
     gene            9493..10026
                     /locus_tag="PKH60_02080"
     CDS             9493..10026
                     /locus_tag="PKH60_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16868.1"
                     /translation="MSLDHVWETREPTDEIIGKRETTAEKAFALIVDALTLLVSKIEA
                     GGDEAEQLKTVVRGAQKSIEALTDTSKQLTSMVGSKENFTLGCYRLIHELEEDGEYVD
                     KAIVAAARALSEFEQLETQVQAKRANQQIESARSMHERMRIRERLDSRTIALTDDDKL
                     EVYLQLLKNTIQGQDIT"
     gene            complement(10019..10717)
                     /gene="uppS"
                     /locus_tag="PKH60_02085"
     CDS             complement(10019..10717)
                     /gene="uppS"
                     /locus_tag="PKH60_02085"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNQ16869.1"
                     /translation="MNSDIQHVAIIMDGNRRWAKQKGLSPTDGHREAAVKTIEPLIQE
                     CVDQKIPYVTFWAFSTENWKRDKEELSAIFNVFRFALKNLAIKFIKEGVKLNILGEVN
                     RFPDDIAKQCLDFVNKTRKNSKITVTFALNYGGRAEIIKAVKKIVEQGLDSDEITEEL
                     FTSNLNTSEIPDPDLIIRTGGEQRLSGFMPWQSIYSELIFTPTLFPDFSVEQFRLALE
                     EFRQRERRFGGDAK"
     gene            complement(10791..11750)
                     /locus_tag="PKH60_02090"
     CDS             complement(10791..11750)
                     /locus_tag="PKH60_02090"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNQ16870.1"
                     /translation="MIGRSEHVAYKDILSGIPVLESRPIPGFTKYPDFRGTIVNPDLE
                     EQLFLLRPPSEEATNRRGIRVLADTSRIIDGERQLIGHLANYDEVLPDGAQIFELPVT
                     RAWGNNLELVSHLARQHRVIQIRGGKHVGQSIAPLGLLDNSSAHIDGDYDDRADQIDL
                     KLIQTALDHPEIFLDATCRGFQLVLAHLLGLLPEEVPNHRSAPGDPATIHQITNVVKK
                     LYGPHAPVALRTLHQFDVNSYHMQGFRLEMIEPHLDVLKENGFYVTHVSQEDEVVEML
                     FRLNKRGEVSGLLKQFHPEKMKGNPVGDLFIAWQKEAIRRAVL"
     gene            complement(11767..12270)
                     /locus_tag="PKH60_02095"
     CDS             complement(11767..12270)
                     /locus_tag="PKH60_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16871.1"
                     /translation="MLETFQLKGIGRSMVQLLFVGTMLIEFYTIFLLVQSGGRDYFLF
                     IMIFVFGLILKYLQGYLVYRIQVDVKNKSIVLNRSFGEVSIVSKDVKEWGYRIIVTEA
                     EYISRTKNLEIYLNNGQKIIYPFNLFGTKPTDEFKQALIDCLGSTPKTYSMKSLTFQS
                     DVLFVLL"
     gene            complement(12264..13127)
                     /locus_tag="PKH60_02100"
     CDS             complement(12264..13127)
                     /locus_tag="PKH60_02100"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNQ16872.1"
                     /translation="MELFDGIAFAKQREQHLKERVSQLIQRGSRPSIASIVFTEDPGS
                     RLYTQKKLEAAQRVGIDFQPIFFSLQDATQQVIDAIDKLNTDLSVTGVMIQKPSKQLW
                     EKRAGEGDFSKWWHSLVMNIKPKKDVDGLTPTTQQAVKDGTWIAKGLVLPATAQAILL
                     VLEQAQVSKTDSIAIIGRSDIVGSPTFFALKKLGYQPILLGRADLANRIASDQQLKEF
                     DVVISATGVEHLVKGSMLKQEVILVDVGEPKPDIDRQSVENIVTFLSPVPGGVGPITV
                     VCLLENVLKLC"
     gene            complement(13181..14095)
                     /locus_tag="PKH60_02105"
     CDS             complement(13181..14095)
                     /locus_tag="PKH60_02105"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HNQ16873.1"
                     /translation="MTKKRLIGAHVSSAGGHDKAIERAADIGCNCVQVFSGSPRVWQR
                     PSLAGIDTEKLYAKQKELSVKPIFTHALYLINLTAEKPEIITKSVETLTKELEFDAHV
                     NGAGVVVHLGSHLGNGWETVRQDLVDRLDMILNTTPKNSRLLIENSAGQQGKLCSDLS
                     EIRWLLDELEKLGQFVSKGRLGWCLDTCHAWAAGYHLGPHPPQETTSKNGNQGSAFEV
                     ISEHGLWSTLKVIHVNDSRDPFASGRDRHANIGDGTIPQQDLQHFLNQPELLNIPLIT
                     EVPGIDGNGPDQVNIERIKRLVKSKSNF"
     gene            14115..14831
                     /locus_tag="PKH60_02110"
     CDS             14115..14831
                     /locus_tag="PKH60_02110"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNQ16874.1"
                     /translation="MPTSKLPPYTIEYSNSEEYHYLKREIWSGHCYYTELEVEDRAPV
                     IVDAGAHIGISTLYFAKHFPTAQIIAIEPNPITRELLENNIWNNRLEDRVSVLPVALS
                     NQTGSAPLFHLPTKQWQLNANLTPQAWNGDKLAESLEVETITLSSLISQPTDIVKLDI
                     EGAELLVLKESRDVLHFIQHLWVEFHPSGKNSLPELTQLLIKQGFKITIWQKEKIVSL
                     ENVRGLCLIHATKNQLPPGE"
     gene            complement(14814..16133)
                     /gene="glyA"
                     /locus_tag="PKH60_02115"
     CDS             complement(14814..16133)
                     /gene="glyA"
                     /locus_tag="PKH60_02115"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNQ16875.1"
                     /translation="MPKDKIFHLIKLEEQRQLDKIGLIPSENAVSPEVAGVLSSCLSN
                     KYAEGFPGRRYYEGNQVIDQIEVLAQERIKQLFSVPYVNVQPYSGSPANMAIYFALME
                     PGDTLMGLKLAMGGHLTHGHPKVTASGRFYNSVQYGVTENARIDFAEVERLALEHKPK
                     VIVAGNTAYPFELEFEKFRKIADKVGAWLVADISHVTGLVIGGEHQSPVPYADVIMST
                     THKTFRGPRGAMILVTDRGLKRDPDLGKKIDSAIIPGLQGGPHNATTAAIAIAAAEAT
                     RSEFKAYSKQIKKNAQVLAKTLLKEGIQLVGGGTETHLMVVDLSSFGPGLGLQVAYAL
                     DVAGIYANRNTVPTEPSSPFYPSGLRLGTPLVTTRGMKEPEMEQIGRWIATVVKHTTN
                     KQLPKDLKQRRAFIKEFKTWADQDQLLKQICSEVKTLASEFPLFTWG"
     gene            complement(16304..17470)
                     /locus_tag="PKH60_02120"
     CDS             complement(16304..17470)
                     /locus_tag="PKH60_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16876.1"
                     /translation="MKITDSREGYNNKEHLFYAENEKRAIELFLLSEFWLLNEQEVVL
                     NEVETIINRLYSAFLLSIDVEELGALIEDSEEIVLRIINASLNDRKHQGLAELIGYLN
                     TIYGRSVIKNAQPVRVPGEEPAVADAKAVARHKIQSLLDDFMVNEENSELFSELNGRN
                     LIFSGDVVAIASPLTERGGELVPNPEVAPEHLLNLSRITDSGNQVEVQSFKKYLEKLY
                     ASGPFQLTYTIANASAMLIRHMTDEGEKVEIGQKINDTGVEVVIEFETLPQELIDNHF
                     AKLRGIDMADKATLLEGGIDPGINVGLPLLDANSPFLKFIKAVSVRPLKETNIQVEEG
                     REHPDAEFGQTIQQFDRVAVRVSDLDIDQQHLLFTSLQSWIVHGISPSAPTLVR"
     gene            complement(17629..18219)
                     /locus_tag="PKH60_02125"
     CDS             complement(17629..18219)
                     /locus_tag="PKH60_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNQ16877.1"
                     /translation="MNSQISIPSFSFPPDETTYVAPTWNEMEQLTIAISKQIINADKP
                     IDLIVTLAKGGWPMTRSLADFLQIDEIASIGVKFYSGINEKLPQPNVYQDLPIDVAGK
                     NVLLFDDVADSGGSLKFVSELLHQRNVASVTTATLFYKEHSVVKPDYFAFTANAWIIF
                     PFEKREMGELLTQKWQKLDLQEEQINHYLKNMGLVW"
     gene            18275..>18593
                     /locus_tag="PKH60_02130"
     CDS             18275..>18593
                     /locus_tag="PKH60_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16878.1"
                     /translation="MIKVLLFDLSNTILFAKDKNYVGGLNALHNQLQATVPNFAFPDY
                     FELDMNLLSMLQSLQNSYVMALFTSETIQNSPAIKPTLEKVFNPIISAQTVGFHKTNP
                     EAYK"
BASE COUNT         5397 a         4145 c         3484 g         5567 t
ORIGIN      
        1 agacattttt atctgctcct atctctgtac tgataaaaat tggtcaagac tagtttgtca
       61 aaggtggggg gatgtggacc gaagggggct cgaacccctg acctcctgca tgccatgcag
      121 gcgctctagc caactgagct accggcccaa gatttcaaga tggatagtat atatcattta
      181 tctagatact tgaactagtg gagtcaataa ttgatttaaa gataaaacaa ctttgtcact
      241 atagccgcaa ctacaaaaaa agtaaattat ttaggatttt tttatatgat ctatcaatct
      301 ttttttattt acagaggaat tagcagagta gggaatagag tgctaatttg gcactagatc
      361 attttagatc agaatgaaac ttgtgatggg cttgtttgag ctgcggcctc gtgatgtggg
      421 tataaatttg agtggttcca atatctttat ggcccaacat ttcttgaacg tctcggagtg
      481 aggcgccatt acttaaaaga tcagtcgcaa aagaatgacg aatgccgtga ggggagacac
      541 gaattggcaa ccgagcttta cgacagtacg catcaaccat ccgttgcaca cttcttgcag
      601 ttagtcgcat ctcctcacca tctgagagaa tatcaggttg cttacctgaa aaacgaatga
      661 ataccggatc ccaattatct cttcgcgtgt tgagccaacg ctctaaccat tgcttagcac
      721 gatcactcag gaaaacgacg cgcggcctcc tgcctttgcc aatcacacca aactcttgcg
      781 aagccaagct aacttgatct cgatttaagt taaccagttc actcacacgc aaaccagtag
      841 aaaataaaac ttccagtaag gctttatctc taaggccaga ttgtgatgat aggtctggtt
      901 gtccgagcaa gcgttcaata ctttctatgg gtaaaaattt cattaagcgt gcttctgctt
      961 tgggtagatc aattttttct ggctgaagta ctggtacgtc attttttacc aaccacttta
     1021 accaagatcg caagccaatc acgtaataac tttgagtccg tttagagaga aggtttccct
     1081 gatcgtcttc gtatcttgct agatatatac gatattttct aactacttcc ttgtctatct
     1141 gtctcacatc agttatctct agatctttta gccaagtgca aaaacgtctc aaatataatg
     1201 aatagttccg aatagtcagt cggctcacat tgcgttcgat ttccaagtgc tccagatact
     1261 gcgttagtaa caaaaatggt tctgtaggta gtgacatatg tttttgtatc tagattcttg
     1321 agttactaca ggtcattagc agatgacaat atttcttgct aattcattga cgagttggtt
     1381 tagcttgaat catcaattac cttcaacctt atgtattgtt gctttgatta ttcaatatca
     1441 aatcgattaa atattttgtc ggtcgttaaa taaatcactc gtcttatgtc tatttgccac
     1501 tatatcagaa attagttaaa acctccattt actttaaata tatgaccttt tgcttgtctt
     1561 atcaatttat tatgtaatct gctatggcta ctaagcgctc aaatagccca aaaacggctt
     1621 tttactggca tagatgatca gttgttcatc gtaaacaaat ggttactatg cacgatgacc
     1681 ttatatttag aatattagca atgtgagtga aaataaagaa ttaaaaaaca aaccaaagag
     1741 ttgtataggt gagattaaat tgatcgaata aataaaagaa atatatcttc gaacaaaact
     1801 atgcggaaga aacggaattg agacgcaaaa tatgctatac ctaatttttt gtatactacg
     1861 tcgtaaaagt aatattgtgc gacgtaatag atgtatgaag ttggctgcct attgcggtag
     1921 gggaagtagc cccctgttta attccaaaaa gtattttttt cactgatcca gttgcagcga
     1981 agttgtacgc acacaaccct atctctccaa ggacctattt aatctgtttt aagtccatat
     2041 ttgctcaaat tctataggaa tttgtcattt agcctggaaa ccaggcgatg tccttgaggt
     2101 gagtatgact gcttcgggtt atttattccc gcatcactac tgcacctcgt attacgaatc
     2161 acacaaaata ctcgagtaat aggcttataa taaattagtt ttccacattc aattcagtat
     2221 caaactatat catttatacc catatcaaac tatatcatta tcccccgctc cctctggaaa
     2281 attatttatt actcccccac tttgctttat tttttatcac aaaacttcat tttatttttt
     2341 tattcgtttt aatctataaa atcttagcct cttaagctga aagtttcttt tttgattagt
     2401 gaaccgcatc ctaatacttg attatcttaa ggcagattcg acttctgtcc ccgactcccc
     2461 cctaccaata aattaaaaat attgatatat caatacaaaa taacatctaa tctggataac
     2521 aatcattcaa ggtctactta ccaggaaaaa gcaagagaaa taaaagtata gacactccag
     2581 ttactaaaga gtattccctg atggtcttaa taaacagtct gcccttgaga taactctgga
     2641 aaaccccaag accaatatat aaaagcgaca taattaataa accaattgcc catacagcgt
     2701 agggaatgaa cgaaagtgca atagaaagct caaatactgc cagtgtaagt aggcctgtga
     2761 gcgcccaatc ggttcgatcc aacttgctcc tcagctgcac actccaaata gacatagcga
     2821 tgagtggaaa atggaaagca gccactaagc ctgctgtaac tgcaaaaggt aggttaaaag
     2881 taaaaagcgt gttagtaatc aaaaatgata taagtaagga aaaaaataat gctatcgttt
     2941 gtgctgcata caacaattgg atggttctcc ccttcgctac tgaaaaaata tttcccgtga
     3001 gcagtaatgc atacatacca acgccaaaca aaacgataaa aagtactctt ttgggcatac
     3061 tagcgggcaa tacaaaataa aaacttgcaa ttgccagcgc atagagagtt ggtagtggaa
     3121 gtattgtcag gtactcattt ttttgtagat cttcagaaag ggcccaagca gctaagctat
     3181 aagtcacacc gccaaatagt aatagcgacc aaactctcca agccgttgga atcaacaaca
     3241 cgccaattag cgcaaagctt aatacgagtg aagaaatgat aaatttttct cttcttctca
     3301 tgaattttgc tctaaatcaa ttttagctgt gcaatacacg tcttcaacat cttcatgatc
     3361 aagtagggtg gaaatgagtg tctggaccgc ttcaagttgc tcagaactta actcaagcgg
     3421 catgcttgct cggtactcag ctccatcagt gcgttcaaac aggtaccttg cagaacctgg
     3481 accagaaatt gatccaccgt ggcgggaaaa aataaatttc atttctgaac ttgagcgatt
     3541 gggattatcg gttaccgcct gaaccaaata tgcttctcca tatggaccaa atgcttcata
     3601 tagcacctct tgaattgtta ctccactcgc agattttccc gatcccttat ctatagcctt
     3661 ctgaattttt tctctaggca tgttagccat tctggcctta tcaagaaaag ttcgtagtgt
     3721 tggattaaat tgcggatcgc cggagccacc ctcttttact gctttgcgta tgtgtttagc
     3781 caaatcacca aatatcttac ttctctgact gtcaaccgct cctttgcgat ttttaatgtt
     3841 gtgccatttg ctatgacctg ccataaaatc aatcctctct gtggtattca tcgaattgta
     3901 caccatttaa caattgttgg ttgacatttg aagctatcat cggtattctc tgcagaacgt
     3961 ttttgctata taaaaacaat caaattatgt ctgcagcaac tgggtcactc aaacaactcg
     4021 ccgtactggc cgccaagcaa gccgaatggc aaaaagcgat agagcttaat caagcaattc
     4081 ttgaaaagga acccaccgac gttggtgcct taaatcgcct tggagtttca cacttgcaac
     4141 tcaatcaagt agcggaagca aaaaaagctt tcaaggtagc cattggtctg gataaattca
     4201 acaccatcgc aaaaaaaaat cttgataaat taaaccaaag tgattcgacg aatcaaacac
     4261 tcaccttttc gaaacaacaa ttcattgaag aatcgggaaa gaccaaggta atcgagcttc
     4321 atcgattggc ggggaaacaa gttcttgaaa cgctttctgt aggtactaaa tgtcagttca
     4381 tgtgtaaaaa acgtttcatt tcaatcgaaa ctaacggtac ctatattggt gcccttccag
     4441 aagatatttc ttttcgactt tcaaaattaa ttcaaaatgg caatcaatat gaatgctata
     4501 tccgttctgt ttcatcaagc acctgctcag tctatgtacg cgaattgttc cgctcagaag
     4561 ataatcttga cgtgagttca ttccccatta atcgcagcgc ccactttgct ggagacgaca
     4621 tcgatgatag tttgcttaaa gaggatgctg cactggaata tgcagggttt gaaaacgaag
     4681 aagctgagga aactgaggaa ccagcagatt tagagtgata ttttctgcaa ttgctctgct
     4741 gaagacgaac ctaattggac cagcactccc acgcgatcag aagaaaatag ttcgcttata
     4801 acctccattt taacctctcc tggcaatgaa gcctgcaaca acgatgccac taaattgcac
     4861 tctgtagtta attgatcatc aataaaaata gttgtttcat cagtagtcac ctcacggttg
     4921 gttacccgca caaccaatcc accctgtttt gcaactagat ctgcatactt accagccact
     4981 cccgaaatac ttgttgtatt ggcaattcca atgggacatg tctctccaag tctgcccaag
     5041 ataaccagct ccgaagcagc atgctctggg ttttctataa tttgtactcc ttcatcctgc
     5101 agtaattgcc aaattggtaa taatcgccag gcttctttaa attttccgag tttcaacagg
     5161 tttgtaagat tagtttgaat cgttcttttt gtagaatggt tatcagcaaa attagtattc
     5221 tcgagtagaa ttatctgatt aactaacacg cctgttgctg ctgcaacttc ccaaggtttg
     5281 tgcaattcgc cagccatgct agaggacggc acgataatta gttttgaata tgttttttct
     5341 ggatcaagtt gagcaatcaa gatcgaacct gattgaccgg cctttggtga atcagtaatt
     5401 gcaatgactt gattttgaaa tcccttcggc acccatcgca atatggcgtt gatacttaga
     5461 gataaagtga cccatagggc aattaaaact aaagcaagaa ataaaaacag actccaccat
     5521 cgttttcttg gttttgttga tcgaagtcta actgagtgtt tttttttgcg ggcgggcatg
     5581 tttgtgctgt ctattattac aggggttagg tgctttgcac aatccaatct ctcatttctg
     5641 ttgccaaacg aaccatgact ctcagattgt gaatgcttgc taatcgataa aagctaagtt
     5701 ctgcaacatt gtacagatgg tgtaaatacg ctctggtgta ccctcctctg caagttgagc
     5761 aatcacaatc tgcctgaagt acgttatgat ctgtcgcaaa tctttgatta ctgatcgcta
     5821 atctaccttt gggaaactct gatttgaaca caggtgtttt ggtagtggta tcaaggtggc
     5881 caacaaataa cgcgccattc cgagccaacc tagttggacc aacgcaatca aacatatcaa
     5941 aacccatgag tatggcctcg atcacatctt gtggatctcg gcccatgccc atggcgtacc
     6001 ttggtgcctg ctcaggcaac aacttaccaa tcgaggccat tacatgctgt gtaccagcca
     6061 aatcgtagcc cactgtttcg ccaccaagcg catagccatc aaaaccaata tcaagcagtg
     6121 aacgaagtga tttctcgcgt aaaacaagtg aggttgaacc ctggacaata ccaaataggg
     6181 cttgatactt tccgtacaaa ctttgtttct tcgcactctc ccactctatt ttgcagcgtt
     6241 gagcccatct tgtagtccgc tcaacagagt cgttcatcat tttttcagaa cttgagtctg
     6301 gcagacattc atcaaaaacc ataataatgt ctgctccaag tgattgttga atttgaatcg
     6361 aagtctctgg ggtcattaca tgcttcgagc catctaggtg agaggtaaat atgactcctt
     6421 cttcagaaat tttgactgta gaagccagac ctttggctcg ctgttgttcg cccagagaaa
     6481 aaacttgata accaccagaa tccgttaaca ttgggtgatc ccaacgcata aattgatgca
     6541 ggccaccagc cgcttctata atctttgtac ctggttttag atagagatgg taggtgttac
     6601 ccaagattat ctgcgcctcc agttgctcca agtctagaga gtctaggctt ctgacactcg
     6661 ctcgagtacc cactggcata aaaatcgggg tcttgatttg gccatgcgga gtcgttatac
     6721 tccctgctct ggctagacct cgagaagtga tcagcgaaaa acgaaatgag tttggcatac
     6781 agtaaacaca tatcctacca tgctatcaat caaccagttc gactataccc tccctccatc
     6841 gctcatttcc catgcgccac ttacaaaacg caccgctagc cgcctgcttt cactagatag
     6901 aacgtcgaaa aagataaccc atcatcactt cagtgactta gctgcgctct tgcagccagg
     6961 tgatgtgctc attcgcaacg aaacaaaagt cattcctgcc cgattatttg gccaacgaaa
     7021 atcaggtaaa aaaattgagt tgttgctaat tagtccagtc gacgacagtg gttttaaatg
     7081 gaagtgcttg actaagcctg gattaaatca aaatgaaaaa atcctgttta attccgaaat
     7141 tgaagcaatg aatacttctc ccaaggaaag ttacgtcaaa gaagttgagt ttggatgttc
     7201 caaaaccgag ttacgtgcgt ttattgtgaa aaacggacac accccactcc ccccatatat
     7261 ttccaaccaa tccaacgaac tgctcttacg aaaacagtac caaaccacgt atgcaaaaca
     7321 tgagggctct gttgcagcgc ccacagccgg attgcatttc accaaagaac tagacaaaga
     7381 attggcttta cgagacattt caattctccc aatcacccta catgttgggt taggcacctt
     7441 tgcgccactc aaagaaacag tttttgcaga aaaacaactt cacgcagaat ggttttcggt
     7501 cagcgatatg acggctcaac aaatcaacca agctaaacaa gaaaagcgac gaattatagc
     7561 ggtggggacg acatcagccc gcacgctcga aagttgcaca aattctaagg gactaattga
     7621 accgaaaacc agcgaaactg ctctttttat ttttccaccg tataaatttc ggagcgtgga
     7681 tggattgata accaattttc atttacctaa atctagccta ctcaccttgg tttcggcatt
     7741 tgttagtgca ccaaatacac cagaaaattt cacatcattc aataagagtt tgattggtaa
     7801 atcataccaa acagcaataa gtgagcaata tcgattttat agctttggtg atgcaatgtt
     7861 gataacctaa acacccaatt cgtagcgcac aaacttgctt acagcaattt tttcgccaat
     7921 tttaccactt aattctttga ctaagtagct gatggttgcg ccaccgtcgc gaatatattc
     7981 ttgctcgagc aactcttcca cattttctgg attcatagct gcaacttgca tagcgatatt
     8041 tttaccaaga gcttgaaatt ccgcattacg tgccacaaaa tctgtttcgc aaactaattc
     8101 aactaatgca gccactcggc caccactatg aacatacgaa aaaattgcac cctctttagt
     8161 ttccctgtcc gactttttgt cggctttggc taaaccacgc tcttttacca acgccatggc
     8221 ttgctgataa tttccctctg tttctgcaag tgccctctgg cagtcaagta ctcccgcacc
     8281 cgtctcttct cttaattttt taatatcggc agcattaata gacattttta ctcctttttc
     8341 tttgctgaag tttttgtaac tttggcagat ttagtggttt tttttacttc tttgacaact
     8401 ggttttttga ttactttagg actagaagct ttaactgttt tagcagttac tttttgctcc
     8461 actgccggtt ttacaacttg ttttgctaca ggttttgtca cctctttagc tactattggt
     8521 tcaacagttt gtttttcttc aggagtagtc acaactgctg cttttacggt ctcagcaaca
     8581 ttaattttgc ttggctctac tccaccagct tttccagcta catacgcctt tgacatgtgt
     8641 tgtaaaaagc actcaatact tttgattgca tcatcattac ctggcacagc aatgtctacc
     8701 aagtctggat ttgtattggt atctaccaac gcaatcactg ggacaccaac tttttccgct
     8761 tctgccacaa ctatcttttc ttttcctggg tcaatgatga ttaatgctgc aggtaaatcg
     8821 ttcatagagc gaataccatc aaaaaatctt ttagctcttg agacttcttt ttcaattttc
     8881 actaattcgt acttagtcct gccttttagg ctaccttcag ctaattttgc ttcggtatca
     8941 accatatctt tgagagattt tttgacctga gaccaattgg tcatcaatcc acccaaccag
     9001 cgagcggtaa tgtgcatcat gcctgaagtt tctgcagcct gttttactaa ttcacgcgct
     9061 tggcgtttgg taccgatgat gataactgat ttcttctggg ctccgagttg atatagtaag
     9121 ttgtacgcca agcgcatttg ctgagcggtc tttgccaaat caatgatgtg aacgccattt
     9181 ttctcactat gaatgtactg ggtcattctt ggttgccact tacttttgtg atgaccataa
     9241 tgcataccag caccaaacaa ttcttgcaga tcaaaatctg gtgctttatc tggaagtgaa
     9301 gtaacgtcta acataacttt gtccttcttt ctagtcgatt tttttcgact aagaggttta
     9361 tttggtaaaa tcaggaccgt acaccaaatc aacccaactt tttacaaggt ggatagtata
     9421 tcacaccaat caagccgagg cctcagaggt gtcaacttgg atattttcgg acaacgaagt
     9481 atactttctc gtatgtcatt agatcatgtg tgggaaaccc gagaacctac tgatgaaatt
     9541 atcggaaaga gagaaacgac ggcagaaaaa gcttttgccc ttattgtaga tgcattaacc
     9601 ttgctcgtga gcaaaatcga ggcaggtggc gatgaagcag aacaattgaa gacagtagtc
     9661 cgaggtgctc aaaaaagcat cgaggctctc accgatacct ccaagcaact tacatcaatg
     9721 gtcggttcta aggaaaattt tacgcttggg tgttatagac tgatacatga attagaagaa
     9781 gatggtgaat atgttgataa agcaatagtc gccgcagcgc gcgctttgtc ggaatttgaa
     9841 caattagaaa ctcaggttca agcaaaaagg gcaaatcagc aaatcgagag cgcgcgcagt
     9901 atgcacgaaa gaatgagaat ccgtgagagg ctagattcaa gaactatcgc tctaacggac
     9961 gatgataaac tggaagtata tttacagtta ctgaaaaaca caattcaagg ccaagacatt
    10021 acttagcatc accgccaaac cgtcgctctc tttgacgaaa ttcttccagt gccaatcgga
    10081 attgttccac cgaaaaatca ggaaacaacg tgggagtaaa aattaactct gaatatattg
    10141 attgccaagg cataaatcca gaaagcctct gttctccccc cgttctgata attaagtctg
    10201 gatctggtat ttctgaggtg ttaaggttag atgtaaataa ttcttcagtg atttcatctg
    10261 aatccaaacc ttgttccaca atttttttta cagcttttat aatttctgct cttcccccat
    10321 agttcagtgc aaatgtcaca gtaatttttg agttcttgcg cgtcttgtta acaaaatcta
    10381 aacattgttt agcaatatca tctggaaaac gattgacttc gccaagaata tttaatttaa
    10441 ccccttcttt aatgaatttt attgctaaat tcttgagggc aaaccgaaaa acattaaata
    10501 ttgccgagag ttcttcttta tctcgcttcc aattttcagt tgaaaacgcc caaaaggtga
    10561 catagggaat tttttgatcc acacattctt gaatgagagg ttcgatggtt ttcaccgccg
    10621 cttcacgatg accatcggtg gggcttaaac ccttttgttt agcccatcgg cgattaccat
    10681 ccataataat cgccacgtgc tgaatgtcgg aattcataag gaatcattat agcaaatcct
    10741 tcattttaaa tcttaagtgc atcacactat ttgttgggta aagttacctt ctacaaaacc
    10801 gctcgtcgta tagcctcctt ttgccaagct ataaataggt cgcccactgg atttcctttc
    10861 attttttctg ggtgaaattg cttgagtaat ccgctgacct cacctctttt attgagtcga
    10921 aataacattt ccacaacttc atcttcttga ctaacatggg tcacatagaa accattctcc
    10981 ttaagaacgt ctaggtgggg ttcaatcatt tctaaacgaa atccctgcat gtgatagctg
    11041 ttaacgtcaa attgatgtaa agtacgaagc gcaactggag catgaggccc atatagtttt
    11101 ttcacaacgt ttgttatttg gtggatagtt gctggatcac caggagcaga tcgatgattt
    11161 ggtacttctt caggaagtaa gccaagtaga tgtgctaaaa ccaattgaaa tcctctacag
    11221 gttgcatcta aaaaaatttc gggatgatct agtgctgttt ggattaactt caagtcgatc
    11281 tgatcagctc gatcatcata atcaccatca atgtgggcgc ttgagttatc aagaagccct
    11341 aatggagcta tcgattgtcc cacatgtttg cctcctcgaa tttggatcac tcgatgttgt
    11401 cttgccaagt gcgagaccaa ttcgaggttg ttaccccacg cacgagtcac tggtagttca
    11461 aaaatttgag caccatctgg cagaacctca tcgtaattgg ctagatggcc aattaactgt
    11521 ctttctccgt cgataattct tgatgtatcg gctagcacac gaattcccct ccgattggta
    11581 gcctcttcgc tcggtggtcg gagcaaaaac aactgttctt caagatctgg gttaactatg
    11641 gttcctctaa aatctggata tttagtaaaa cctggaatcg gtcgagattc caaaactggt
    11701 attccagaca aaatgtcttt atacgcaacg tgttcagaac gaccaatcat agagacatat
    11761 agtaaatcaa agtagcacaa aaagcacgtc tgattgaaag gtgagtgatt tcatcgaata
    11821 cgtctttgga gttgaaccca agcagtcaat cagagcctgt ttgaactcat ctgttggttt
    11881 agttccgaat aaattgaagg gatagattat tttttggcca ttattaaggt aaatctcaag
    11941 gttttttgta cgactgatgt attctgcctc agttactatt atccgatacc cccattcttt
    12001 gacgtctttc gatactattg atacttctcc aaaagacctg tttaacacta tcgatttgtt
    12061 cttcacatca acttgaatac gataaaccaa atatccttga agatatttta aaattagacc
    12121 aaaaacaaaa atcatgatga aaagaaagta atctctgcct ccagactgta ccaacaagaa
    12181 tatggtataa aactcgatga gcatcgttcc gacgaacaac agctggacca tacttcgacc
    12241 gattcctttg agttggaatg tttctaacat agcttaagga cattttccaa taaacatacc
    12301 accgtgatgg gacccacacc accaggaaca ggagataaaa atgttacgat gttttcaacc
    12361 gactgacgat ctatgtctgg tttcggctcc cccacgtcta ccaaaattac ttcctgcttc
    12421 aacattgagc ctttaactaa atgctctact ccagtggcac taattacaac atcgaattct
    12481 tttaactgtt ggtcagaagc aattctgttt gctaaatcgg ctcgacccag aagtattggt
    12541 tggtacccaa gtttcttgag ggcaaaaaaa gttggtgagc caacaatatc tgacctaccg
    12601 atgatcgcga ttgaatcggt tttcgaaact tgagcttgtt caagcaccaa taaaattgct
    12661 tgtgcggttg ctggcagtac taatccctta gcaatccaag tgccatcttt gaccgcttgt
    12721 tgagtagttg gggttaaacc atcgacatct ttttttggct taatattcat aaccagcgag
    12781 tgccaccact tagagaaatc accttcccct gctcgttttt cccaaagttg cttggaaggc
    12841 ttttgaatca tgacgcccgt gacgctcaga tctgtattta gtttgtcgat tgcgtcgata
    12901 acttgctgtg tagcatcttg cagcgaaaaa aaaattggtt gaaaatcgat ccccaccctc
    12961 tgggctgctt ctagcttttt ttgtgtatac aaccgactac caggatcttc ggtgaacacg
    13021 atagaggcta tcgatggcct actgcctcgt tgaatcaatt gactcacccg ttctttcagg
    13081 tgttgttccc gctgtttagc aaaagcaatg ccatcaaata attccatagc agtattgtac
    13141 agtgaaatat atttttaaga gtacttctct ctctgacaac ttaaaaattc gactttgatt
    13201 ttactaatcg tttgattctc tcaatattta cctggtctgg accgttacca tcaattccag
    13261 gaacttcagt aatgagcgga atgttaagta gctctggttg gttgaggaag tgctggaggt
    13321 cttgctgagg aatggtacca tcgccaatgt tggcgtggcg atccctgcca gaagcaaagg
    13381 gatcgcgact gtcgttgacg tgaattactt ttaaagtcga ccataaacca tgctcagaaa
    13441 tcacttcaaa tgctgaaccc tggttgccat ttttgcttgt agtttcctgg ggcggatggg
    13501 gtcccaagtg ataaccagca gcccaagcgt gacaggtatc taggcaccaa cccagtcgtc
    13561 ctttactcac aaattgacct aatttttcta gttcgtctag caaccaacgg atttctgata
    13621 aatctgagca aagtttgcct tgctgaccgg ccgagttttc gatcaacaat cgactgtttt
    13681 ttggagttgt attcagaatc atatctagcc gatcgaccag atcctgcctg acggtttccc
    13741 aaccgttacc caaatgacta cctaagtgga ctaccactcc tgctccattt acatgtgcat
    13801 cgaactccag ttccttcgtg agagtctcaa ctgacttcgt aattatttcg ggcttttctg
    13861 cagtcaaatt gatcaaatat aaggcatgag taaatattgg tttaacagat aattcttttt
    13921 gttttgcata cagtttttct gtatcaattc cagctaaaga tggacgttgc caaacccgtg
    13981 gtgaacccga aaagacttgg acacaattgc agccaatatc tgccgctcgt tcgatggctt
    14041 tgtcatgccc acctgccgat gacacatggg caccaattaa tcttttcttt gtcatatgcg
    14101 gtacactata ccatgtgcca acatcaaaat taccacccta caccatcgaa tattccaata
    14161 gcgaagagta tcactatctg aagcgcgaga tctggagtgg tcactgttac tacactgaac
    14221 tagaagttga agatcgggcg ccggtcatcg tcgatgccgg cgcccacatt ggtattagca
    14281 ccctctactt cgccaaacac ttccccaccg cacagataat cgccatagaa cctaatccta
    14341 ttacaagaga gcttcttgaa aacaatatct ggaataatcg tttggaagat cgagtttcag
    14401 tactaccagt cgccctctcc aaccaaactg gttctgcccc actctttcac cttccaacca
    14461 aacagtggca actcaatgcc aatctaactc cgcaagcttg gaacggtgac aaattagccg
    14521 aatcgcttga agtcgaaacc attacactca gctcactgat ttctcaacct actgacatcg
    14581 tcaaattgga tattgaaggg gctgaactac tagtcctcaa agaatcgaga gacgtactac
    14641 attttattca acatctctgg gtcgagtttc atccctctgg aaagaattct ctacccgaat
    14701 taactcaact gttaataaaa caaggtttca agataaccat ttggcaaaaa gaaaaaattg
    14761 tttccttaga aaatgttcgt ggactatgct tgattcacgc caccaaaaac cagctacccc
    14821 caggtgaata gtggaaactc actcgccaaa gtcttaacct ctgagcaaat ttgcttgagc
    14881 aactgatctt ggtctgccca agttttgaat tctttaatga atgctcttcg ttgcttaaga
    14941 tctttgggta gctgtttgtt agtagtgtgc tttacaactg tggcaatcca ccgtccgatc
    15001 tgttccattt cgggctcttt cataccccta gttgtaacta atggtgtacc caaacgcaaa
    15061 ccagaaggat aaaatggtga acttggttcg gtgggcacgg tatttcggtt ggcatagatc
    15121 cccgctacat caagcgcata tgcaacttgc agtcccaaac caggtccaaa tgagctcaaa
    15181 tcaaccacca tcaaatgtgt ttctgtaccg ccaccaacta gctgtatacc ctctttaagc
    15241 aaggtctttg ccaacacttg agcatttttc ttaatttgtt tggaatacgc tttaaattcc
    15301 gaccttgtcg cttctgctgc ggcgatggca atcgccgccg tggtagcatt gtgaggccct
    15361 ccctgcaaac caggaataat cgctgagtca atttttttgc ccaaatctgg atctcgcttc
    15421 agtcctcgat cagttactag aatcattgca ccacgagggc cacgaaacgt tttatgggtg
    15481 gtggacataa tcacatcagc gtacggaact ggagattgat gttcaccacc aatcaccaaa
    15541 cccgttacat gcgaaatatc tgccaccaac cacgctccaa ccttatctgc aatctttcta
    15601 aatttttcga attcaagttc aaaaggatac gcagtattgc ccgccacaat cacttttggt
    15661 ttgtgctcta gggcaagtcg ctcaacttca gcaaaatcta ttcgcgcatt ctctgtcact
    15721 ccatactgca cggagttata aaatctgcca gaagcagtta cttttgggtg tccgtgagtg
    15781 agatgtcctc ccatcgccag cttaagcccc atcagggtat cacccggctc catcaaggca
    15841 aaatagattg ccatgttcgc tggcgaccca gaatagggct gcacattcac atagggcact
    15901 gaaaacagtt gtttgattct ctcttgagcc aacacttcaa tctggtctat aacttggttt
    15961 ccttcatagt atcgtcttcc cggaaatccc tcagcgtatt tgttggacaa acaagacgat
    16021 agtactccag caacttctgg agaaaccgca ttctcagatg gaattaaacc aattttatct
    16081 agttggcgct gttcttctag tttgattaag tgaaaaattt tgtcttttgg catcaaaact
    16141 acctttcgta ctaactgaat agtaacacag aaaagaaaaa ctaaaaaaac taacgggcag
    16201 atggcaggca gccgagttta aactacgaat gtaatcgtaa caaaaaaaca aaacggggga
    16261 aagatgtatc actgggtcag aatgggttaa gttacacaag tatttatcta actaacgttg
    16321 gtgctgaagg tgagattcca tgtacaatcc agctttggag tgaggtaaac aaaagatgct
    16381 gctgatctat atccaaatct gaaactcgca ctgccactcg atcaaattgc tgtatggtct
    16441 gaccaaattc ggcatctggg tgttctctgc cctcctcaac ttgaatattc gtttctttca
    16501 gtggccgaac ggaaacagct ttgatgaatt ttaaaaatgg tgagtttgca tctaacagag
    16561 gtaggcctac attaatccct ggatctatcc ctccttcaag caaagtggcc ttatctgcca
    16621 tatcaatacc ccgtaacttt gcaaagtgat tatcaatcaa ttcttgaggt aaagtttcaa
    16681 attctattac cacttctaca ccggtgtcat ttattttctg accaatttca actttctcac
    16741 cttcgtctgt catgtgacga atcagcatag cggatgcatt tgctatggta tacgttaatt
    16801 gaaatggtcc tgacgcatac agtttttcaa gatatttttt aaagctctgg acctcaactt
    16861 ggttacctga gtcagtgatt ctagacaaat tcagtaagtg ttctggagca acttctggat
    16921 tgggcacaag ttctccgcct ctttcggtaa gtggcgaagc aattgcaact acatctccag
    16981 aaaaaatcag gttgcgtcca tttaattctg aaaataattc agaattttcc tcattaacca
    17041 taaaatcgtc cagtaaactc tgaattttat gcctggccac tgcctttgca tcagcaacag
    17101 ctggctcttc tcctggcaca cgcaccggtt gggcattttt tattaccgac cgaccataaa
    17161 tcgtattaag atagcctatt aattcagcta atccctgatg ttttctatca ttgagtgaag
    17221 cattgatgat gcgcaaaaca atttcttccg aatcttcgat taaagcacca agttcttcta
    17281 catcaattga aagtaaaaag gctgaataca gtcggttgat aatcgtctcg acttcattca
    17341 gcactacttc ttgttcgttc aatagccaaa attctgaaag taaaaatagc tctatcgctc
    17401 tcttttcatt ctctgcataa aacaggtgtt ctttattatt atatccttct ctggaatctg
    17461 tgattttcat aggttacaaa aaatcctcac cgttactttt actcagcttg gtgtcactct
    17521 aactgtggtg agcagctaga tgcgtttctt gggagcaaaa gtcctttgtg aggcttctgc
    17581 tttgttttgg ttgctttaac caaaaaccct atgtattagg cgtgtatttt accacactag
    17641 gcccatattt ttcaagtagt gatttatctg ttcttcctgt aaatctaatt tttgccattt
    17701 ttgtgtcagc aactccccca tctctcgctt ttcaaacgga aaaataatcc aagcgttggc
    17761 ggtaaaggca aaataatctg gttttaccac agaatgttct ttatagaaaa gggttgcggt
    17821 agtcactgaa gcaacatttc tttggtgcaa taattcagaa acaaatttta gcgacccccc
    17881 gctgtctgcc acatcatcga atagcaagac gtttttccct gctacatcaa ttggtaaatc
    17941 ctggtacaca tttggctgcg gtagtttctc gttgatacca gaataaaatt tcacaccaat
    18001 actggcaatt tcatcgattt gcaaaaaatc agccaaggat cgcgtcatcg gccagccacc
    18061 cttggcaagc gtcacaatca ggtcaatagg tttatctgca ttgataatct gcttagaaat
    18121 tgcgatcgtg agttgctcca tttcattcca agtcggtgca acataggtgg tttcatctgg
    18181 gggaaaagaa aagcttggga tcgaaatttg cgagttcata ctatatggta caccacgaac
    18241 ctgatacttg tgaagccagt gctatactca agtcgtgatt aaagtattgc tttttgattt
    18301 atccaacact attttatttg ccaaagataa aaactatgtc gggggactga acgctcttca
    18361 caaccagctt caagctacag tgccaaattt cgcttttcct gattattttg aattagatat
    18421 gaatcttctc tcaatgttgc aatctctaca aaatagctat gtaatggctc tttttacatc
    18481 tgaaacaatt caaaattcac ctgcaattaa accaacttta gagaaagtct ttaatccaat
    18541 tatttctgct caaaccgtcg gttttcacaa aacaaacccg gaagcatata aat
//
LOCUS       DAOQZP010000019        18588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_3364_length_18588_cov_5.488588, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000019 DAOQZP010000000
VERSION     DAOQZP010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 18588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18588
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3364_length_18588_cov_5.488588"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..893)
                     /locus_tag="PKH60_02135"
     CDS             complement(87..893)
                     /locus_tag="PKH60_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16879.1"
                     /translation="MATKDQKIKSLELENIDLGEKLNGVTEIAADLILGLAVAVNMLE
                     EKQAMLDLDALMLSVAQEAIGQKNDRIYELERAERRRQETEQREARSKAKLAEVRNTP
                     SLVDWALVNVETTADGLNIKATPDRWGRKRVTTTQINSDGSNGATVAITKGKADLTFR
                     IPIFGSFEVGAKVTHTETSSTPDSPFVEIENEMVFKFKMGGKKYRVTITAGGEVRRGC
                     GDWYANTYRSTTRIEYWCDSCQEWHYQDDFRTNVNTSPMTERRSGGCGGW"
     gene            1341..1787
                     /locus_tag="PKH60_02140"
     CDS             1341..1787
                     /locus_tag="PKH60_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16880.1"
                     /translation="MLLVAQFILQFLPLFWRPIILNFDAGSVTLDGFVFASLLTFLVA
                     VACFEACIETIVTRYGTGLVIDVPVTAALFKVIIQVPVAAVLAQSVAVIRLSNAPVMT
                     VLIVRATVIPASVEVVRARLVAVQDAIFPVSRDYSKVILDVHSSKM"
     gene            2307..2696
                     /locus_tag="PKH60_02145"
     CDS             2307..2696
                     /locus_tag="PKH60_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16881.1"
                     /translation="MLEASRNVPQKPVLRLNLGGEGEILGCIQVNLPYVLEAGDNFVA
                     SRNGDPIGIKGVLRGGPLVICDSDRLPFLPGVFDEIFANGVPIDRTTWLGPGYSGKEI
                     CRVCKHHSQKPCDACHHGIFPIKEGLI"
     gene            2696..5008
                     /locus_tag="PKH60_02150"
     CDS             2696..5008
                     /locus_tag="PKH60_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16882.1"
                     /translation="MSEEQRVAISELRHLPELVFQFARASIGPIRSGVYFAAVHLPGL
                     RQLAIRRQPHTENFLYHKKDPEQTPLGQCAEVLTAIDERLYNQGEPEHYIGQELFTQF
                     EQVREQLQVEAIQQLGKPGKLSDYVFFDRQTQQNVLFFQTEENQLTPIVVNQEFDAKS
                     LQFKEAIENEMPASYLDEELRLDEVLHEMGFAEWSTDTQAYSLRRVTSICNEFHLTRQ
                     ADLEHDGILINNLRDKGVHTIGELQSVFRLLFSKGIQSGQDGKTYVDQDLLVRARKIF
                     CLAYPEIAQEHAEQWICEAAQERLYFHTITKKIRQNTALVLAFMQSRAFSQHAQAFKD
                     RHLASLGQTERETAIAMLAGLYVLHYEGNPPDQSRSQYYLAKEVSYFEDIEQFLADYS
                     NAGGITPELIDSESKQEQEVVCGVIETTEPFDQQKQEVIDKFDASYFGVRCQMRRHRT
                     HQALLGTLQKVRVPITVDPRKGMFPNLIANLAIKRVKTDEGRYHLLSSTWLWQNGGYL
                     LEDGETPPDFNEQKSKVNPETDELEPVGTRLDRSLQYIFGIDQELGAVENGSVYDKYQ
                     AQEQRIISEAPTIMEYLATDEAVPESIRDFISQIISGEFVTRVLEKFGSISDQDPNII
                     GRFFPEGLQAFTQSVSYRYAFDFVRGVETNLGLQNGFLARIRAQFVGDWKPLDAAKLS
                     LEKLTIAKQRTLQDGEHRLAWMNHETSKIKTLKDLQEKAEIIFGWDTDDMKFLRWVPF
                     WDVWNTLGGAWDCVCFTRKTIFNPRAFKAA"
     gene            complement(5230..6252)
                     /locus_tag="PKH60_02155"
     CDS             complement(5230..6252)
                     /locus_tag="PKH60_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNQ16883.1"
                     /translation="MLQPQYALTPQILTNLTQIERLYGQLEGMRIPQKLELNLERDHL
                     IKSTYISNSIEGNPLSLPEVTNLLLGDRVPANRDEQEIKNYFDLLKSLDQYASQSLSI
                     DIITKLHKRLLKAVDTEIAGTIRNTRVVVGGYQTKNGIPTLHVKHEPPFHKKTEIEQA
                     LVDMIDWIESDTKTPPLLKIGIFHHHYVYIHPFVDGNGRTCRLLTALLFLRYQYQINK
                     YFVLDDYYDLDRFAYSDMLSSADTGDSTLWLAYFTDGVKYSLQSALARVENAVQSGKV
                     AERPTPKEQQVLTYLSSHLEVTSQDLVAEFSISRQQAHKLLAGLVEKGYVEKKGDTKS
                     SYYKIK"
     gene            complement(6365..6436)
                     /locus_tag="PKH60_02160"
     tRNA            complement(6365..6436)
                     /locus_tag="PKH60_02160"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6401..6403),aa:Glu,seq:ttc)
     gene            complement(6453..6809)
                     /locus_tag="PKH60_02165"
     CDS             complement(6453..6809)
                     /locus_tag="PKH60_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNQ16884.1"
                     /translation="MEKNNFYSGDQAEHLAQQFLIDHGFSMLEHNVRVKNHEVDIVAV
                     DQEQNELVFVEVKSRATGYYGDPSQAVDYAKLRSIQYVAACYRGQVNYQGDFRFDIIT
                     VVDGTIQHYQNVTWNR"
     gene            complement(6796..7149)
                     /gene="rplS"
                     /locus_tag="PKH60_02170"
     CDS             complement(6796..7149)
                     /gene="rplS"
                     /locus_tag="PKH60_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNQ16885.1"
                     /translation="MAQFFTHQEQKVSVGDSVGIHQEITEGGKKRIQIFDGLVIAIKN
                     RGIGQSFTVRKIATGGIGVEKIFPTALPSITKIVVKRTGKVRRSKLYFLRERTGKQAT
                     KVKEKKTLLKAVGEK"
     gene            complement(7220..8902)
                     /locus_tag="PKH60_02175"
     CDS             complement(7220..8902)
                     /locus_tag="PKH60_02175"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNQ16886.1"
                     /translation="MSNHGNNSPNQTKFIFVSGGVLSGLGKGVTAASLGLLLKNRGYS
                     VGNIKCENYLNLDSGNINPIEHGDVFLCEDGLEADLDLGTYERFLEVEVGHKNFTTMG
                     QIYSKVIENSKNLQYDGATVDAIPYVPEEAIRRIQESAQGFDFILVELGGTAGEYQNA
                     IYYEAYRIMKLRFPQDVLHIHVTYFPKPSHINELKSKPTQLSVRTLNSMGIQPDFIVG
                     RAELVIDEKRKEKVAFYCNLRTDEVISNPDCDSIYEVPIVFAEQQFDEKVLNKLNLPP
                     NKQDLTAWKDLVSVIQHKRSQPVTVAIIAKYIATGDYELEDSYQSLVESLKIASWHSD
                     VDLRISFINAESLEKEGKKAQEDLAKAHGIIVPIGWGSRGVEGKIAAIKYARENKVPY
                     LGLCYGMQLACVEFARNVAHLTDANTAEVDDKTPHPIIHQIPFEEQYQRIKGEGASMR
                     LGAYDCILKADTLAHAIYQKHQAFKDEKTNLISERHRHRYEFNNEYRGVLADHGLIIS
                     GTSPDDFFVEMIELPKSMHPFFIATQAHPEYKSRPLHPHPLFTEFLAAAKKR"
     gene            complement(8946..12416)
                     /gene="dnaE"
                     /locus_tag="PKH60_02180"
     CDS             complement(8946..12416)
                     /gene="dnaE"
                     /locus_tag="PKH60_02180"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNQ16887.1"
                     /translation="MASRAPTNFVHLHVHTEYSMLDGLCQLPKLVEQVKTLNQPAVAL
                     TDHGVMFGALHFYNACLKADIKPIVGVEAYIAKKSHLDKQTKMGADQAHLTLLASNYV
                     GYKNLLKLVSTAHLEGFSYKPRIDEELLAQHNGGVIVLTGCASSPINLLLRNGEVDAA
                     KSKLEWYKSIFADRLYVEIQRHPQVPFLAELEKHQLALAREQHIPIVATNDVHYISSE
                     DARAQDALLCVSTHKLISDKDRMSMMDSPDYYLRSTQEMIELFHDLPEAISNTLEIAN
                     RCDLTIPTGQLIFPEYAIPEGSTTEQFLHDLAFERAKQKFGELKPELVKRLDYELNII
                     NDKGYGTYFLITQDFVNWAKQQGIGVGPGRGSAAGSLVSYCLGITSIDPIAHGLAFER
                     FLNPERPTPPDIDMDFADDRRDEVIEYVASKYGADRVAHVITFGRMEARVAIRDIGRV
                     LGFPYEDPDRIAKLIPNQPGHRTSLSQAVQSVPELAAYYNQPKFRELIDLAQKVEGNV
                     RHSSVHAAAIVITDKPMSEYTAIQLDSKTGKTITQYDMYALDCNISDDAIGLLKFDFL
                     GLRNLSIIQSTLNLVKQYRTESIAIDEIPLDDPKTFALLASGDTMGVFQLESSGMRRV
                     ARSLRPSQFSDIMALVALYRPGPMDLIPQFIEGKHNTNKITYAHDSLKPILKETYGVM
                     VYQEQILEIAHVMAGYSLGEADILRRAIGKKKKKLLDINRQRFINQSVQNSYSKAVAE
                     KVWGFIEAFANYGFNKAHAASYAMIAYQTAYLKANYPIEYMTALMSVESGSTAQTRDE
                     KISQAIEECKKMDISILPPNINHSARDFTIEKLPSGKKKYAIRFGLNAIKHVGSAAID
                     TIIAVRDSLGSFTSLTQFIAETDSRKVNKTTLECLIKVGAMDSFGTRASMLENLEEIR
                     KRISGLGSSDQGQTTLFGEIGGGELAMQDTFVALPEYPQAELLSFEKELLGLYLTEHP
                     MAKSLKTAEKQANKKIAEVDGAVHQDQRFRFGGVITKLRVVRTKKNDKEMAFGNLEDT
                     SGHIRFVVFPKTYDEYKSTLAVDSVVVITGRIDVREEETQLVVESISVPNSFDVQQSI
                     EESSFQIFIPRKTSKETLEKLGALLKQHPGKDTVVIVIPNGAKPQMMTLPYGVTWSDE
                     LKTAVEALLA"
     gene            12481..12900
                     /locus_tag="PKH60_02185"
     CDS             12481..12900
                     /locus_tag="PKH60_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16888.1"
                     /translation="MSLVEVRSPQPEIVIVPTNAKVVEQLPSLSEAASKYLSELAETL
                     DASEAYEIVYPAGHQYGYAGLSAEFPALSSTGVIENLRWIFQHAQLQSVQECQQYVVQ
                     PLEDPLGIITQRYVVDSDGRIRKVTVPQRLRPPSKVT"
     gene            complement(12857..13147)
                     /locus_tag="PKH60_02190"
     CDS             complement(12857..13147)
                     /locus_tag="PKH60_02190"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNQ16889.1"
                     /translation="MTKNSNPKLSNLAQKVFGEVSKIPRGSTTTYKAIAQTVGTSPRA
                     VGNILHTNPDPNTYPCHRVIRSDGKLASGYAFGGKTAQLMLLLKEGVVVEAQ"
     gene            13181..13867
                     /locus_tag="PKH60_02195"
     CDS             13181..13867
                     /locus_tag="PKH60_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16890.1"
                     /translation="MNIKDAERPTQHEQKSHLTQDQRALVNVLKNVELSTAMEIVPPW
                     MQPLLKLEMNRRISEESLEDIYKAAKIIAEALSYLGMSNFLSQVQFEQYLETVENSIE
                     IELVELESENQEDQDEGVDNGISSDYVDEDPEKYEVQEDFPDEEISWEDYISFQLMRL
                     QYNLIIFEGMISNFEEMLIKQTLSVETYVQKLSNDVLSALKEVLSQSHLQKAFSLTNK
                     NYSPSDLFIQ"
     gene            complement(13853..15160)
                     /gene="gatB"
                     /locus_tag="PKH60_02200"
     CDS             complement(13853..15160)
                     /gene="gatB"
                     /locus_tag="PKH60_02200"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNQ16891.1"
                     /translation="MRHLICGLEVHAELKTNSKMFCGCKNDPFGASQPNIYTCPVCLG
                     LPGALPVANKKAIEWTIKLGLALNCKINLFSKFDRKHYFYPDLPKAYQISQYDLPFCE
                     NGFVETSEGRVRIRRIHLEEDTGKLLHKTIDGNKVSLIDFNRSSVPLIEIVTEPDIHT
                     ASQAKEYAKKLRQILRYLDIANCDMEQGGMRLEANLSLANTPDVLPKYKVEVKNINSF
                     RFMEQAIAYEVIRQTELLDKNQTPAQETRGWDMQKQVTYSQRVKEEAEDYRYFPDPDL
                     PPIEFTQTQIEIMRKLLPELPDVIARRWEHDFNLDPKNSAYFLETQSGAQWADAVFAD
                     AKRNKVEPNDVANLIINKKVTVDQTTPVTEVIEKILAATRTDQLDPNLVKEAINSIKN
                     EFPTEWQRYQAGDKKLLGFFIGQLKKKINQPLDPKQIIELLNK"
     gene            complement(15157..16554)
                     /locus_tag="PKH60_02205"
     CDS             complement(15157..16554)
                     /locus_tag="PKH60_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015173828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNQ16892.1"
                     /translation="MTKKPISLNTVTIKQALTGLQAKDFSLAEIYEATDQIVAKQNKD
                     LNVYLATNEKASVEARKVAESPLAGLPIAVKDNFGTIGLPTTAASLVLDGYQSQYEST
                     VTTKLLEAGGVIYGKTNMDAWAHGSSTETSQYGRTLNPRNTQHLPGGSSGGSAAAVAA
                     DMCLGALGSETAGSIRQPAAWCGVVGLKPTYGRCSRYGVVAMASSTDSPGPIGKTVED
                     TAILLNAIAGHDPYDSTTSQEAVPDFTSFLDKSIKGMKIGVLYHDLPGLDEIWPHFDA
                     ATKVLSDLGALVEPVKAFDPHHAIGVYTIVQRAEVSSNLGRYDGIRFGNDRSHFGDEA
                     KRRIMLGTFTLSSGYSDQYYLRAQKVRTLFINDFEKLLKKYDALISLTSPGYAKKIGA
                     SEGAAMFGELEDLLLEPSSISGLPGISVPCYRDPNTNLYLGLNIMTPKWQEGRAIQIA
                     DAYEKATTWNSWRQP"
     gene            complement(16551..16874)
                     /gene="gatC"
                     /locus_tag="PKH60_02210"
     CDS             complement(16551..16874)
                     /gene="gatC"
                     /locus_tag="PKH60_02210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNQ16893.1"
                     /translation="MTSKKQTDFSEKQVEHTAKLAHIPVVATETKQLSEAFSTTLEVI
                     SDLSEVPVEGVEPTHHVTGLKNVTREDVVDKKRMFSQAQALANAPKSYEGFFVVPRII
                     DRDEE"
     gene            complement(16924..17835)
                     /locus_tag="PKH60_02215"
     CDS             complement(16924..17835)
                     /locus_tag="PKH60_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNQ16894.1"
                     /translation="MTHYQLVAQNKRKSWLVIVGFTIFVGVAAWLMTYSFGYDLSLVG
                     VALIFSGMISFGSYWFSDTVVLAVSKARPATREEFFDFYTVAENLSMSLQIPVPKLYV
                     IDDTAMNAFATGRDPKHSAVVATTGLLSRLDRGEIEGVVAHELAHVTNYDILLMSIVS
                     ILVGFIALLADWMLRISFLGGGKRRDSRDSGQIEMILGLVGFVLVLLSPLIANLIKLA
                     ISRKREFLADASGVAITKNPMGLISALSKISQDQEPLEAANKATAHLYISDPLKNQKG
                     GVGWFANMFNTHPPVEERIQALQSIVV"
     gene            complement(17832..18431)
                     /locus_tag="PKH60_02220"
     CDS             complement(17832..18431)
                     /locus_tag="PKH60_02220"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNQ16895.1"
                     /translation="MLSGILPILLFILIGIGFYVVSVYNGLQKFKTQIAASIQEIGNQ
                     LKRQASLIPNLETSVEGYLKHEKGIYDAITSARKAVQAADKSGNMNDMDKAVNQLQSM
                     IPQIKVLVESNPELKADKTVTKFMDELTDTSDKLMYSRRTLIDLTQMYNVKLVTFPSN
                     FIAGLFGFTAEKGLVTPTSGEHIEVSSKEMEDVKVNLQK"
BASE COUNT         5137 a         3855 c         4073 g         5523 t
ORIGIN      
        1 aacgcaaaaa gccccacctt gcggtggggc ctttcgctct tcagttttgc ttgctattta
       61 gttttttccc cccccagggt ccctggctac cagccgccgc agccgccgga gcggcgctcg
      121 gtcatggggc tggtgttgac gttggtgcgg aaatcgtcct ggtagtgcca ctcttggcag
      181 ctatcgcacc agtactcgat cctcgtcgtt gagcggtagg tgttggcata ccagtcgcca
      241 cagccgcgcc ggacttcgcc gccggctgtg atggtgacac ggtacttctt gccgcccatc
      301 ttgaacttga agaccatctc gttctcgatc tccacgaacg ggctgtcggg ggtgctgctg
      361 gtctcggtgt gcgtgacctt agcacccacc tcgaagctgc cgaaaatggg gatgcggaac
      421 gtgaggtccg ctttgccctt cgtgatagcc accgtcgcgc cgttgctgcc gtccgagttg
      481 atctgggtgg tggtgacgcg tttgcgcccc caccggtcag gggtggcctt gatattcagg
      541 ccgtcggccg tcgtttcgac gtttaccaaa gcccagtcca ccaacgaggg cgtgttgcgc
      601 acttcggcca gcttggcctt gctgcgggcc tcgcgctgtt cggtctcctg gcgccggcgc
      661 tcggcgcgct ccagctcgta gatccggtcg ttcttctggc cgatggcctc ctgcgcgaca
      721 ctgagcatga gggcgtcgag atcgagcatc gcctgcttct cctcgagcat gttgacggcc
      781 accgccagac ccaggataag gtcggccgcg atttccgtca cgccgttcag cttctcgccg
      841 aggtcgatgt tctccaactc caaactcttg atcttctgat ccttcgtagc catgagattt
      901 gcctccttaa ggctgggccc gcaggcccga ttgttggggg aaatagtttt tgactcggca
      961 gactatgggt ctgccggatg tccattgtga caacgtgctg tgtacgctgg tgtatacaca
     1021 agacgctgtc acaaaaaaga caactaaagt cataaacttt tattatattt agcaaacaaa
     1081 ctgttaaaga gcgttgtgtt aagagactga tcgttattcg tttcgatgat gaagcgaaaa
     1141 caatatgtgt ctgttaaccg ttattgcata cgaatatgta acaacaacgt agaaatatat
     1201 cacaatagta tattactgtc aataggaaaa ctattaggat gtagattata ttcagttaat
     1261 aaacgcaaaa agcctcaccc ttgtagggat gaggcctttc gctcttactc agttttgttt
     1321 gctatctttg ctaggatgaa gtgctacttg ttgcgcaatt catcctccag ttcttgccac
     1381 tcttctggcg tccaatcatc ctcaacttcg atgcaggcag tgtcacactc gacggcttcg
     1441 tcttcgcatc cctcctcaca tttctcgtcg ccgtcgcatg cttcgaagca tgcatcgaaa
     1501 cgatcgtcac acgctatgga acaggtctcg taattgacgt accagtcact gccgccctct
     1561 tcaaggtcat catccaggtc ccagtcgccg ccgtcctcgc acagagcgtc gcagtcatca
     1621 gactcagcaa cgcaccggtt atgacagtcc tcatcgtcag agccacagtc atcccagcat
     1681 ctgtcgaagt cgtccgagca cgcctcgtgg cagtccagga cgccatcttc ccagtctccc
     1741 gggattattc caaggttatc ctcgatgtgc actcgagcaa aatgtaacac tagcatggtt
     1801 cgcctccttt aggcattcga gctttcaggc tcggtttggg ggaaatcggt ttttgattcg
     1861 cctgacaggg gtcaggtggc tgtcagtcgt gacagcgtgc tgtgtacacg aaaatataca
     1921 caagacgctg ccacaaaaaa gacaactaaa agtcataaac cttttattat atttagcaaa
     1981 caaactgtta aagagcgttg tgttaagaga ctgatcgtta ttcgcttcga tgatgaagcg
     2041 aaaacaatat gtgtctgtta accgttattg catacaaata tgtaacaaca acgtgtaagt
     2101 atatcaaaaa atgcataaag ttacaataat attattataa tgattgtatc aggatactcc
     2161 cgaaattttt gatgactaag attaatcgtg ctattatgga ttagacatta ttacaatata
     2221 acctataatg ttgactttat gttgtaccta gaatatacta taggtgtaat aaatatgtaa
     2281 gaaattgaga cacagacata aaagctatgc ttgaagcaag cagaaatgtt ccacaaaaac
     2341 ccgttcttcg gctcaaccta ggaggtgagg gtgaaatctt aggatgcatt caggtcaatc
     2401 ttccatacgt attagaggct ggtgataact ttgttgcctc aagaaatgga gacccaattg
     2461 gaataaaagg tgttctaagg ggtgggccct tggttatttg tgattctgat cgattgccat
     2521 ttttgccagg agtgttcgat gaaatctttg ccaatggagt gcccatcgat aggactactt
     2581 ggttgggtcc aggctatagt ggtaaggaaa tttgtagggt ttgcaaacat cattcgcaaa
     2641 aaccctgtga cgcttgtcat catgggattt ttccaatcaa ggaaggactt atttgatgtc
     2701 agaagaacaa agggttgcta ttagtgaact gagacatctg ccagaattag tatttcagtt
     2761 tgctagggct tcaattggtc ctattagaag tggagtctac tttgcggcag tgcacttacc
     2821 tggattgcgg cagttggcca tcagaagaca gcctcataca gaaaatttcc tttaccacaa
     2881 aaaggatccg gaacaaactc cacttggaca atgtgccgaa gtcctgactg caatcgatga
     2941 gcgattatac aatcagggag aaccagagca ttacatcgga caagaactat ttactcaatt
     3001 tgagcaagtg cgtgagcaat tacaggtaga agcgattcaa caactgggta aaccaggcaa
     3061 actttcagat tatgtttttt ttgatcgcca aacacaacaa aatgtcctat ttttccaaac
     3121 tgaggaaaat cagttgactc cgatagttgt aaatcaagaa tttgatgcaa aaagtctgca
     3181 atttaaggaa gctatcgaaa acgaaatgcc cgcctcatat cttgatgagg aattgcgtct
     3241 tgatgaagta ttgcacgaaa tgggttttgc cgaatggtca acagatactc aagcctactc
     3301 actgagacgt gttactagta tttgtaatga gtttcattta accagacaag ccgacctaga
     3361 acacgatggt atcttaataa ataatctcag agacaaagga gtacatacca ttggagaact
     3421 tcaatctgtt ttccgacttc ttttttctaa agggattcaa tcaggccagg atggtaaaac
     3481 ttatgtagac caagatttac tcgtgagggc tcgtaaaata ttttgtcttg cgtatccaga
     3541 gattgcacag gagcatgccg agcaatggat ttgtgaagca gcacaagaaa gactgtattt
     3601 tcacaccata accaaaaaaa ttcgccaaaa tacagcactt gtactggcgt ttatgcagtc
     3661 acgggctttt tcgcaacatg cgcaagcatt taaagataga cacttagcta gcttgggtca
     3721 aaccgaacgc gaaacagcta ttgcaatgct tgccgggttg tatgtactgc actatgaggg
     3781 taatccacca gaccaatcta gatcacagta ttatttggca aaagaagtaa gctactttga
     3841 agatatcgag cagtttcttg ctgattactc taatgcaggt ggtataactc cagaattaat
     3901 agattctgaa agtaagcaag agcaagaggt ggtatgtgga gttattgaaa cgacagagcc
     3961 ttttgatcag caaaaacaag aggtaataga taagtttgac gctagttatt tcggtgtccg
     4021 ctgtcagatg cgcaggcatc gaacacatca agctctactt ggaacgttac agaaggttcg
     4081 ggttcccatt accgtagatc cgaggaaagg tatgtttcct aatttaatag ccaatttagc
     4141 catcaagcgg gtcaaaaccg atgaaggtag atatcatttg ctaagctcca cgtggctttg
     4201 gcaaaatgga ggatatctcc tagaagacgg agaaactcca ccagatttta atgagcaaaa
     4261 aagtaaagtt aatccggaaa cagatgaact cgagcctgtg ggcacccgat tagatcgctc
     4321 attgcaatat atatttggca ttgaccaaga gctaggcgct gtagagaacg gatcagtgta
     4381 tgacaaatat caggctcaag agcagcgaat aatttctgaa gctccaacaa tcatggaata
     4441 tttggccacg gatgaagctg tgccagaaag catcagagat tttatcagcc aaattatttc
     4501 tggtgaattt gtgactagag ttttagaaaa atttggtagt atttctgatc aagatcccaa
     4561 tattattgga aggtttttcc ccgaaggatt acaagcgttt acgcaatccg tttcatatcg
     4621 atatgctttt gattttgttc ggggtgtgga aacaaacttg ggattacaga atggattttt
     4681 ggctagaatt agagctcaat ttgttggcga ctggaagcca ctagatgctg caaaattatc
     4741 tttggaaaaa ctgacaatcg ctaagcaaag gaccttgcag gacggtgagc accgacttgc
     4801 ttggatgaat catgaaactt cgaaaattaa aacacttaag gatctccaag aaaaagcaga
     4861 aattattttt ggttgggaca ccgatgatat gaagttttta cgctgggttc ccttttggga
     4921 tgtttggaat accttgggcg gagcatggga ctgtgtctgt ttcaccagaa aaacaatatt
     4981 taatccaaga gcttttaaag cggcttagtt tttgcttggt gatactgaag agtatgttgg
     5041 tgtaaaaaga tcgtttggtg accccaccgt cccaaatttg aaacactttt tcaaaagttt
     5101 aatcgaacta taccacaagc tagtcgagct tgggttttgc tattctagaa tagattaagt
     5161 atggccaact taaaataccc atctgagaat gagtgaattt gttcaaatcg agcaataact
     5221 ggtgtactgt cattttattt tgtagtaact actttttgta tcaccttttt tttcaacata
     5281 tcccttctca actagacctg ccagtaactt atgagcttgc tgacgagaga tgctgaattc
     5341 tgcaaccaag tcttgagaag tcacttcaag atgtgagctt aaatatgtga gaacttgttg
     5401 ttccttaggg gtcggacgct cagccacctt tccactctgt acagcattct ctactcgagc
     5461 cagagcactt tgtagagagt atttcacacc atccgtgaaa taggctaacc acagggttga
     5521 atctcctgta tcagcactgc ttaacatatc ggaataggca aaacgatcga gatcatagta
     5581 gtcatctaga acaaaatatt tgttaatttg atactgatat ctcaggaaaa gcagggcggt
     5641 taaaagccgg caggtgcgtc cattaccatc aacaaatgga tggatatata cgtagtgatg
     5701 atgaaaaatg ccaattttaa gaagcggagg agtttttgta tctgattcaa tccaatcaat
     5761 catatccact agtgcttgtt caatctctgt ttttttgtga aatggaggct catgtttgac
     5821 atgaagtgtg gggatgccat tttttgtttg gtagccacct actacgacgc gcgtatttct
     5881 gatggtacct gcaatttcag tatcaacagc tttgagtaat cttttgtgca gtttggtaat
     5941 gatgtctata gagagtgatt gactagcata ctggtcaagt gatttgagta gatcaaaata
     6001 attttttatt tcctgctcat cccgattggc aggcactcta tctcccaata gtagattggt
     6061 cacctcaggc agagatagtg ggttgccctc aatactatta ctgatatagg tcgacttgat
     6121 caaatggtcg cgctctaaat tcagctcaag cttctgagga attcgcatcc cctcaagctg
     6181 gccataaagg cgttctattt gggtgaggtt tgttaggatt tggggagtaa gggcatattg
     6241 gggttggagc atatagtcag tctacaaaat aattgaacaa ttgtcaacct atatgtaaac
     6301 atatttgtca actctagtac atgacaaaga aggtatcaac ataaaagtag taattgttga
     6361 aatgtgaccc catggggaat cgaaccccag ttacaaggat gaaaacctta tgtcctaacc
     6421 actagacgat ggggccactt aaaaaattca gcttaccgat tccaagttac attttggtaa
     6481 tgctgaatcg taccatcgac gacggtgatt atatcaaacc tgaagtcacc ttggtagttg
     6541 acttgccctc gataacacgc ggcaacatat tgaattgatc gaagtttggc gtagtcaact
     6601 gcttggctgg gatcaccgta gtaaccggtg gctcgagatt ttacttctac aaatacgagc
     6661 tcgttttgct cttggtcaac tgcaacaatg tcaacttcat gattttttac tctgacatta
     6721 tgttcaagca tggaaaaccc atgatcaatt agaaattgtt gtgctaaatg ctcagcttga
     6781 tcgccggagt aaaaattatt tttctccaac tgctttgagg agagttttct tttctttaac
     6841 cttggtggct tgtttgccgg ttcgttcacg caggaaatat aacttacttc ggcgtacttt
     6901 tccggtacgt tttaccacaa tttttgtaat acttggaagc gcagtaggaa aaattttctc
     6961 tacaccaatt cccccagtag cgatttttct tacagtaaag gattgaccaa tcccacggtt
     7021 tttgattgca ataaccaggc catcaaatat ctgaattcgt tttttacctc cctcagtaat
     7081 ttcttgatgg atgccgacag aatcacctac cgatactttt tgctcttgat gtgtaaaaaa
     7141 ttgtgccata gagaaccttt tcaaaaacta gactggtgta ttctactcaa gaggtgttaa
     7201 ttatgcaaat gcaacttgtt tatctctttt ttgctgccgc taaaaattct gtaaagagag
     7261 gatgtgggtg cagcggtctc gacttgtatt ctggatgtgc ctgagtggca ataaaaaagg
     7321 gatgcatact cttgggtagc tcgatcattt ccacaaaaaa atcatcaggt gaggtgccag
     7381 atattataag accatgatct gctaggacgc cgcgatattc attattaaat tcatatcgat
     7441 ggcgatgacg ttcggaaatc aggttggttt tttcatcctt aaaagcctga tgtttttggt
     7501 aaattgcatg tgcaagcgtg tcagccttga gaatacaatc gtatgctccg agtcgcatac
     7561 ttgccccctc acccttaatt ctttgatatt gttcttcaaa gggaatttgg tgaatgattg
     7621 ggtgaggtgt tttatcgtct acctctgcgg tgttagcgtc tgttaggtga gcaacattac
     7681 gagcaaattc cacacaagcc aactgcatgc cgtaacacaa gcccagatat ggaactttat
     7741 tttctctagc gtatttgatg gcggctattt ttccttcaac accccgacta ccccaaccga
     7801 ttgggacaat aatgccatga gcctttgcta agtcttcttg ggctttttta ccttcttttt
     7861 cgagtgactc tgcattaata aaagatatgc ggagatccac atctgaatgc caagaggcaa
     7921 tttttaacga ttcgactaat gattggtacg aatcttccaa ttcatagtcc cctgtggcaa
     7981 tgtacttggc gataattgct acggtcacgg gctgacttcg tttatgctga ataactgaca
     8041 caagatcttt ccaggctgtt aaatcttgct tgttgggtgg taagttgagt ttgttgagga
     8101 ctttttcatc aaactgttgc tctgcaaata caattggaac ttcatagatg gaatcacaat
     8161 ctggattgga aatcacctca tcagtgcgta agttgcaata aaacgcaact ttttcttttc
     8221 gcttttcatc aataacgagt tctgcccgac cgacaataaa atctggctga atccccatgg
     8281 aatttaaggt tcggactgac aattgagtgg gttttgattt taattcattt atgtgcgagg
     8341 gcttaggaaa atacgtgaca tgaatatgca acacatcttg aggaaatcgc aacttcatga
     8401 tgcgataggc ttcgtaatag atggcatttt gatattctcc ggcagtgcct ccaagttcca
     8461 caaggataaa atcaaaacct tgggctgatt cttgaattct tcggatggct tcttctggca
     8521 catagggaat tgcatccaca gtagctccgt catactgtaa attttttgaa ttttctataa
     8581 cttttgaata aatttgaccc atggtcgtaa aatttttatg accaacttct acctcgagaa
     8641 atcgttcata ggtacctagg tctaggtctg cttccaatcc atcctcacat aaaaaaacgt
     8701 ctccatgctc aatggggttg atgttgccag aatctaggtt gagataattt tcgcacttga
     8761 tgttgccaac agaataacct cgatttttca acaataaacc gagagaggcg gcggttacac
     8821 ctttgccgag tccagataaa acacctccag aaacgaagat aaatttagtt tgatttgggg
     8881 aattatttcc atgattgctc atggggcaat attgtaacaa cacaaacggg aaaagtcaca
     8941 cccaattatg ccagcaaagc ctcaacagct gtttttaact cgtcagacca ggtaactcca
     9001 tagggcaagg tcatcatttg gggttttgcg ccgttgggga tgacaataac cacagtatct
     9061 tttcctgggt gttgtttaag tagtgcgccg agtttttcta gagtctcttt actggttttt
     9121 ctgggaataa atatttgaaa gctcgattct tcaattgatt gttgaacatc aaaggagttg
     9181 ggcacactga ttgactcgac aaccaattga gtttcctctt cgcggacgtc aatcctacca
     9241 gtaataacga ccacagaatc tactgctaaa gtggatttat attcatcata ggttttggga
     9301 aatacgacaa atcggatatg tccagaggtg tcttctaaat taccaaacgc catttcttta
     9361 tcgttttttt tcgtcctaac gactctgagt ttggtaatta caccaccaaa ccgaaatcgt
     9421 tgatcttggt gtactgcgcc atctacctcg gcaatttttt tattggcctg cttttctgca
     9481 gtttttaagg atttagccat tggatgctcg gttagataca ggccaagcaa ttctttttca
     9541 aatgaaagta actcagcttg aggatattct ggtaaggcta caaaggtatc ctgcatagcc
     9601 agttcaccgc ctccaatttc tccaaatagg gtagtttggc cttggtctga actaccaagc
     9661 ccggaaattc gcttgcgaat ttcctcaagg ttttccagca tactagctcg agtgccgaaa
     9721 ctgtccatag ctccaacctt gatgaggcat tccaaagtgg ttttgttgac ttttcgacta
     9781 tctgtttcgg cgatgaattg ggttaatgaa gtgaaacttc ccaaactatc tctaacagca
     9841 atgatagtat cgatcgctgc agaacccacg tgcttaatag cgttcaagcc aaagcggatg
     9901 gcgtactttt tcttacctga cggtaatttt tcaatagtaa aatctctagc agagtgatta
     9961 atatttggag gcagaattga tatatccatt tttttacact cctctatggc ctgagaaatt
    10021 ttttcatctc tcgtttgtgc cgtagagcca gactcaacgc tcattaaagc agtcatgtac
    10081 tcaatggggt aattggcttt taaataggcg gtttgatagg caatcatggc atagcttgcc
    10141 gcatgagctt tgttgaatcc atagttagca aaggcttcaa tgaatcccca aaccttttcg
    10201 gctacagctt tgctgtatga gttttggacc gattgattga tgaaacgctg gcggttaata
    10261 tcaagtaatt tctttttttt cttgccaatt gctcggcgta aaatatctgc ttcccccaaa
    10321 ctatatcccg ccatgacgtg agcaatttct aaaatctgct cttggtacac cattactcca
    10381 taggtttctt ttaaaattgg tttgagcgaa tcatgtgcgt atgtaatttt gtttgtattg
    10441 tgttttccct caataaattg gggtatcaga tccattggac cgggtcgata caaagcaacg
    10501 agagccataa tatctgaaaa ctgtgatggt cgaagtgagc gtgccacacg acgcatgccg
    10561 gaagattcga gttgaaaaac acccatggta tccccgctcg ctaacagagc gaatgtcttg
    10621 ggatcatcta gtggtatttc atcaatcgcg atggattccg tccgatattg ttttaccaaa
    10681 ttaagtgtac tttggataat tgaaagattg cgaagcccga ggaaatcaaa cttcagtaaa
    10741 ccaatggcgt catcagaaat attacagtct agtgcgtaca tgtcatattg ggtaatagtt
    10801 ttgccagttt ttgagtcgag ttgaatagct gtatattcac tcatgggttt gtcagtaatc
    10861 acaattgctg ctgcgtgcac ggacgagtga cgaacattac cttcaacttt ttgcgcgaga
    10921 tcgattagct cccgaaattt aggttgattg taataggcag caagttctgg aacgctttga
    10981 actgcctggc tcagtgatgt gcggtggccg ggctggttgg gtattagctt ggcaattcta
    11041 tctggatctt cgtaaggaaa accaagtact cggccaatat cccggatagc cactcgggcc
    11101 tccattcgac caaacgtaat cacatgagct actctatcag caccatattt gcttgccaca
    11161 tattcaatga cttcgtctct gcgatcgtca gcaaagtcca tatcgatatc cggtggagtt
    11221 ggacgttcag gattcagaaa tcgttcaaaa gcgaggccgt gagcgattgg gtcgatagat
    11281 gtaatgccca gacaatacga tactaatgag cccgcggctg agcccctacc tggtccaacg
    11341 ccaatcccct gctgttttgc ccaattcaca aaatcctggg taattaagaa gtaggtacca
    11401 taacctttat cgttaatgat attgagttca tagtctagtc ttttaaccaa ctctggtttc
    11461 agttcgccaa acttttgttt agctcgttca aaagctaagt catgaaggaa ttgctctgta
    11521 gtcgatcctt ctggaatggc atattcagga aaaatgagtt ggccggttgg tatggtgaga
    11581 tcacatcgat tggcgatttc caaggtgttg gaaatcgctt caggcaaatc atgaaataat
    11641 tcaatcattt cttgcgttga gcgcaaatag taatcgggag agtccatcat ggacatgcgg
    11701 tctttgtctg aaatgagttt gtgagttgaa acacacaata gcgcgtcctg agcacgcgcg
    11761 tcttccgaag agatgtaatg cacgtcgttg gtagccacaa tgggtatgtg ttgttctcga
    11821 gcgagtgcca attgatgttt ttctagttcg gctaagaatg gaacttgcgg gtgacgctga
    11881 atttccacat ataaacgatc ggcaaaaatg cttttgtacc actccaattt actttttgcc
    11941 gcatcaacct ctccatttct taggagtaag ttaattggac tcgatgcgca gccagtaagc
    12001 acgattacac ccccgttatg ttgtgctagg agctcttcat cgattctggg tttataagaa
    12061 aagccctcta ggtgggcagt agacaccagt ttgagaagat ttttatatcc aacatagtta
    12121 gatgccagaa gtgtgagatg ggcttggtca gcacccattt tcgtttgttt atctaggtgt
    12181 gatttttttg cgatgtatgc ttctacaccg acgataggtt tgatatcggc ttttagacaa
    12241 gcattgtaga aatgtaacgc gccaaacatg acgccatgat cagttaaagc cacggcgggt
    12301 tgattgagtg ttttaacttg ttcgactaac ttgggaagtt ggcaaagacc atcgagcatc
    12361 gagtactcag tgtgcacatg caggtgaaca aaattggttg gagctcgact tgccatacta
    12421 gtagtatctt agcagataat agttgctaga attaggttgt tactgaaaat tgggaggcat
    12481 gtgagtttag ttgaagttag atcacctcaa ccagagatcg ttatagttcc aaccaatgct
    12541 aaggttgttg agcaattgcc atcgctttct gaggcagcga gcaaatatct ctcagaatta
    12601 gcagaaacac tagatgcaag tgaagcgtat gaaattgtgt atccagccgg acatcaatat
    12661 ggttatgccg gattgtctgc agaatttcct gcgctctcat cgactggagt aattgagaac
    12721 ctgagatgga tttttcagca tgctcaactg caatcagtcc aggagtgtca acaatatgtt
    12781 gttcagccac tagaagatcc tcttgggata attacacagc gatatgttgt tgatagtgat
    12841 ggaagaattc ggaaagttac tgtgcctcaa cgactacgcc ctccttcaaa agtaacatga
    12901 gttgtgctgt ttttccccca aatgcgtagc cacttgctag ttttccatca gatcgaatca
    12961 ctcgatgaca tggatatgta tttggatctg ggttggtgtg caaaatgtta ccgacagccc
    13021 ttgggctggt gccaacagtt tgagcaatag ctttgtacgt agtagttgag cctcgtggaa
    13081 ttttagaaac ctcaccaaag actttttgag ctaaattcga gagtttaggg ttactatttt
    13141 tagtcatatt tattggtaat ttattgtata ctacctcaat atgaatataa aagatgcaga
    13201 aagacctaca caacatgagc agaaatctca tttgacacag gaccagcgag cattggttaa
    13261 tgtgcttaaa aatgttgaac tttcgactgc gatggagata gtcccacctt ggatgcagcc
    13321 tctattaaaa cttgagatga atcgaagaat tagtgaggaa tcattagaag atatttataa
    13381 agcagccaaa attatagctg aagccttgag ttatttaggt atgtccaatt ttttaagtca
    13441 agttcagttt gaacaatatt tagaaactgt agagaattca atagaaatag aattagtaga
    13501 gcttgagtct gaaaatcagg aagatcaaga tgaaggtgtc gataatggga tcagctctga
    13561 ctatgttgat gaagatccag aaaaatacga agtccaggaa gacttccctg atgaggaaat
    13621 tagttgggag gactacatct catttcaact tatgaggtta caatacaact taataatttt
    13681 tgagggaatg attagtaact ttgaagagat gctgataaaa cagaccttgt cagtagagac
    13741 ttacgtacaa aagctaagca acgatgtgct ctcagcatta aaagaggttt taagtcaaag
    13801 ccatctacaa aaagcctttt cacttacaaa caaaaattat tcgccaagcg acttatttat
    13861 tcaatagttc aataatttgt ttaggatcga gaggttgatt gatttttttc ttaagctggc
    13921 caataaagaa accaagaagt tttttatcgc cggcttggta acgctgccac tcagtaggaa
    13981 attcattttt aattgaatta atggcttctt tgactaagtt tggatcgagt tgatcggtcc
    14041 gagtggcagc tagtattttt tcaatgactt cggtaacagg ggttgtttga tctacagtta
    14101 cttttttatt tattatcaga ttagcaacat catttggttc taccttgttg cgtttggcat
    14161 cagcaaaaac cgcatctgcc cactgtgcac ccgactgtgt ttctaaaaag taagcggagt
    14221 tttttggatc tagattgaaa tcgtgttccc aacgtcgagc aatcacatct ggcagttcgg
    14281 gtagtagttt gcgcatgatc tcaatttgag tctgggtaaa ctcaattggc ggcaaatcag
    14341 gatcggggaa ataccgataa tcttctgctt cctccttaac tcgttgagaa taagtaactt
    14401 gtttttgcat atcccatcca cgggtttctt gagcgggagt ctgattttta tcaagcaact
    14461 cggtttgtcg aatcacttca taggcaattg cttgctccat gaatctaaag gagttaatat
    14521 tttttacttc aactttatac ttaggtaaca catcgggggt gttggcaagc gatagatttg
    14581 cttcaagtcg catgccaccc tgctccatat cgcaattagc gatgtctaaa tatctcagga
    14641 tttgtcgaag ttttttggcg tactcttttg cttgcgaggc agtatgaatg tctggctcag
    14701 tgacgatttc aatcagtggt acgctggagc gattaaaatc tataaggcta actttattac
    14761 catcgatggt tttatgtagc agtttacccg tgtcctcttc tagatgaata cgacgaattc
    14821 taactctacc ctcactggtt tctacaaaac cattttcaca aaacggtaaa tcgtattggc
    14881 tgatctgata ggctttaggt aagtcggggt aaaagtaatg ttttcggtca aacttggaaa
    14941 aaaggttaat cttgcaattg agggctaagc caagtttgat tgtccactca attgcttttt
    15001 tattagcgac tggcagagct ccaggtaaac ccaggcaaac ggggcaggta taaatattcg
    15061 gctgtgaagc tccaaacgga tcatttttgc aaccacaaaa cattttggag ttcgttttta
    15121 gttcagcgtg aacttccagt ccacaaatca agtgcctcat ggttgcctcc agctattcca
    15181 agtggttgct ttttcgtagg cgtccgcaat ttgaattgct ctgccctctt gccacttggg
    15241 agtcatgatg tttaaaccga ggtagagatt ggtgtttgga tctcgatagc agggcacgct
    15301 gattccaggt aggccggaaa tcgaacttgg ttcgagtagt aaatcttcca actcaccaaa
    15361 catggcggct ccttcactgg ctccgatttt tttagcatat ccaggactag ttaaactaat
    15421 gagagcatca tattttttta acagtttttc aaaatcattg atgaataaag tgcggacttt
    15481 ctgagcccgt aaataatatt gatcgctata cccactagaa agggtaaagg ttccaagcat
    15541 aattcggcgt ttggcctcat cgccgaaatg agagcgatca ttgccaaagc gaataccgtc
    15601 ataacgacca aggttgctac tgacttcggc gcgctggacg atggtgtaga cgccgattgc
    15661 gtggtgtgga tcaaatgcct tgactggctc taccagcgcg ccaagatccg aaagcacttt
    15721 ggtggccgca tcaaaatgcg gccaaatttc atctaggcct ggcaaatcat gatatagaac
    15781 gccaattttc attcctttaa ttgatttatc taaaaatgaa gtaaagtcgg gtactgcctc
    15841 ttgagaagtg gtgctgtcat atggatcatg tcctgcgatg gcattgagta agattgctgt
    15901 gtcctcgacg gttttcccaa ttggaccggg ggaatctgtt gatgatgcca tcgctaccac
    15961 gccataccga ctgcaacgac catacgtcgg tttgagaccc accacaccac accatgcagc
    16021 tggctggcgt atcgagccgg cagtttcgct ccctagagct cccaaacaca tgtctgcagc
    16081 aaccgccgct gcgctgccac cggaagatcc acccggcaag tgttgagtat ttcgggggtt
    16141 tagggttcta ccatattgag atgtctctgt tgatgagccg tgagcccaag cgtccatatt
    16201 ggttttacca tagataacac cgcctgcctc aagtaatttg gttgttaccg ttgattcata
    16261 ttgagattgg tagccgtcaa gcactaggga ggccgctgta gtaggtaaac caatggtgcc
    16321 gaagttatcc ttgacggcaa ttggtaatcc agcaagcgga gactcagcca cttttctggc
    16381 ctcgaccgaa gccttttcat tcgtagctaa atacacgttg agatccttat tctgttttgc
    16441 gacaatttga tcggtagctt cgtagatttc tgctaagctg aaatcttttg cttgcaagcc
    16501 agtaagggcc tgtttgatgg ttacagtatt gagcgaaatg ggtttttttg tcattcttca
    16561 tccctatcaa tgattcttgg taccacaaaa aaaccttcat acgattttgg tgcgttggct
    16621 agtgcttggg cttgtgaaaa catccttttt ttatcaacaa cgtcttcacg agtgacattt
    16681 tttagtccag taacatgatg tgttggctca actccttcaa ctggaacttc tgataaatca
    16741 ctaatgactt caagtgtggt ggaaaaagct tccgacagtt gcttggtttc ggtcgcgaca
    16801 accggaatat gagctagctt cgcagtatgt tcgacttgct tttcactaaa atctgtctgt
    16861 tttttcgagg tcatatgtgt aggaatcgta ccataccaat caagaaaacc aaagtagaat
    16921 tactcaaacc actatcgatt gcaaagcttg aattctctct tcgactggag gatgtgtatt
    16981 aaacatgttg gcaaaccacc ccaccccacc cttttgattt ttgagtgggt cgctaatgta
    17041 taaatgagct gtggctttgt tagcagcttc taagggctct tggtcttgtg aaattttgct
    17101 taaagccgat attaagccca tcggattttt tgtgatcgca acaccacttg catcagctaa
    17161 aaactctctt tttctagaga ttgctagttt gatcaaattg gcaattagtg gtgagaggag
    17221 cactaagacg aagccaacca gtcctaaaat catttctatt tgcccagaat cacgactatc
    17281 ccgtcgttta ccaccaccca agaatgaaat tctaagcatc cagtctgcca gcagggcaat
    17341 aaaaccaact agaatgctca caattgacat gagtaaaata tcatagttgg tcacatgtgc
    17401 caattcgtga gccaccaccc cttcaatttc accacgatct agtctggaca gcaaccctgt
    17461 tgtggccaca actgccgaat gtttgggatc tctgcctgtg gcaaacgcat tcattgcagt
    17521 atcgtcaatc acatacaatt ttggcactgg gatttgcaaa ctcatgctta aattttctgc
    17581 cacggtatag aaatcaaaga actcctcacg agtggctggg cgagctttgg atactgctaa
    17641 aactaccgta tcagagaacc agtaagagcc aaaggaaatc attccagaaa aaattaacgc
    17701 cactccaacc aagctcagat cgtagccaaa tgaatatgtc attaaccagg ctgctacacc
    17761 aacaaaaatt gtaaagccaa caatgaccaa ccaagatttc cgcttgtttt gagcaacgag
    17821 ttgataatgt gtcatttttg taaattgact ttgacgtctt ccatttcttt ggatgaaact
    17881 tcaatatgtt cgccactggt tggagtgact aaaccctttt cggcagtaaa accaaacagc
    17941 ccagcaatga agttggaagg aaaggtaacc aacttaacat tgtacatttg agtcaagtca
    18001 attagtgttc ttctagaata cattaattta tctgaagtgt cggttaattc atccatgaac
    18061 ttggtgacag ttttatctgc tttgagttct ggattactct caacaagcac tttaatttga
    18121 ggaatcatac tctgcagctg attgacggct ttgtccatat cattcatgtt gccagattta
    18181 tccgctgctt gaacggcttt tcgagcactc gtaattgcat cgtagatgcc tttttcatgt
    18241 ttgaggtaac cttctacaga agtttctaga tttggaatca aagaggcttg tcgcttgagt
    18301 tgattaccaa tttcttggat actcgcagca atttgggttt taaatttttg cagtccgttg
    18361 tagacagaaa caacgtagaa acctatacca atgaggatga acagtaggat gggtaatatt
    18421 cctgacaaca tacagactcc ttcataaaaa acactcagta ctaatcattt atcatactac
    18481 attttgtaaa gagctgttaa tctcacaatt gcttggttta ttgtttggac tgttaacatg
    18541 tttggtaaag taggtattta gaagtaaagc agtctgaaat aagtaaag
//
LOCUS       DAOQZP010000020        18532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_3379_length_18532_cov_5.442063, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000020 DAOQZP010000000
VERSION     DAOQZP010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 18532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18532
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3379_length_18532_cov_5.442063"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..681)
                     /locus_tag="PKH60_02225"
     CDS             complement(13..681)
                     /locus_tag="PKH60_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16896.1"
                     /translation="MKIIPAILTESRSVAISQIELANSDDRFDALQIDIIDGWFADNL
                     TLTPADYADLEYGQLQLDFHLMTEEPLDYVFELLEHRENLPIRSVIAQIEKMSSQITF
                     VDQLKKTGWKVGLSLDLFTPIESIETSVMGELDFVQVMAIEAGFQGQAFVTTIVDKIK
                     ELQGLRQKKSYQYELIVDGGIKKEEVALLQSLEVESVTIGSGLWKSVDFSQAVDTLFS
                     VVRE"
     gene            801..1238
                     /locus_tag="PKH60_02230"
     CDS             801..1238
                     /locus_tag="PKH60_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16897.1"
                     /translation="MLYSQYSRYIKKSDAEFFVKQQYRLIAIMLLFIVIAFGFIYFTV
                     FSKMMTMYEDLGQLQPFLVRQFPYGFTIFSLVAVLSSVRFFTKEVDVKELNKKLSKYK
                     QNEMILSNEVLDMKNAILVIGATMLFVLYMVFSIISPIYSITQ"
     gene            complement(1429..1884)
                     /locus_tag="PKH60_02235"
     CDS             complement(1429..1884)
                     /locus_tag="PKH60_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HNQ16898.1"
                     /translation="MIIYIGADHRGFGLKERLKQWLVTQHLAVVDCGNVRLDPGDDFP
                     VFAHAVAEQVVADEDHRGILICGSGAGVVIAANKVPGARCSVGINLSDIYHARAHDDL
                     NILAIASNFVTFEEAKQQVGMFLNTPFEAVERHVRRLKRIEAIERTYSK"
     gene            1949..2377
                     /locus_tag="PKH60_02240"
     CDS             1949..2377
                     /locus_tag="PKH60_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16899.1"
                     /translation="MKLDLEIQIMPINSERSMHQRPGEGLKHHLADRERLRDYLLWLV
                     FEILKETTTLKASVEISPSPYFKSSRVLPNITLSIVAEDPDSLRECITKITSLSIQKG
                     LTLLRRMSLGASGNDNGNGVEKNNAIFELSLYNNRHRTVS"
     gene            complement(2379..2729)
                     /locus_tag="PKH60_02245"
     CDS             complement(2379..2729)
                     /locus_tag="PKH60_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNQ16900.1"
                     /translation="MKAKKNTQVNKLEKRIWQALRQVLDPELGIDIVSLGLIYRVSVD
                     LTATGQQKVDILMTLTTPGCPLADSFDGLVRQSLSTISTIDSDEDVTITLTFDPPWTQ
                     DMMTDEAKADLGMI"
     gene            complement(2827..3024)
                     /locus_tag="PKH60_02250"
     CDS             complement(2827..3024)
                     /locus_tag="PKH60_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020023356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16901.1"
                     /translation="MAFSYTNAKGQVYYLHMKDVTLKNGRVQRIYFFARDVRAGSLDA
                     VPAGYAVMETKRTNMPVLKRA"
     gene            complement(3137..3655)
                     /locus_tag="PKH60_02255"
     CDS             complement(3137..3655)
                     /locus_tag="PKH60_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16902.1"
                     /translation="MNKFTPYIFPAVVVVIVFFLVFRWYSDRSRLTPDVGEGISIENL
                     SEEELKNAISGVGDYKTIQLDETESSEPNEETLTGVVRYEIENDKVRLSVIAESGDPS
                     VDYYVWMQLSGSTTPKRVFMLVANKGGLVGSGALSVDQLPLDVFVTRGETLDEQMKNV
                     VLKGRVEADTAK"
     gene            complement(3700..4344)
                     /locus_tag="PKH60_02260"
     CDS             complement(3700..4344)
                     /locus_tag="PKH60_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNQ16903.1"
                     /translation="MKNLPLLIGTLVVSIALIAGIAVLFSGQASPVSGEQVDATLLAD
                     GALNSTGPSDAPVTIVEFSDFQCPACKASEPMVKSVLEQYPDQVRLVFRNFPLDQIHP
                     NARLAAQAAQVAQSEGKFWQYAQLLFEKQEEWSDLSSYDLLKTTLSEYASELGIDSAS
                     FLERMESSEVVSAVQRDVDLAKELNIMATPTFFVNGIQTPAPELLSAVKNVLDQ"
     gene            complement(4358..4621)
                     /locus_tag="PKH60_02265"
     CDS             complement(4358..4621)
                     /locus_tag="PKH60_02265"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNQ16904.1"
                     /translation="MAQDQNTVAQKQIGKYQVSVINDKCISAASCVAIAPGVFDLDEE
                     GIARVISQDGNDDETKLLAAQSCPTSAIIVVDTESGQQVWPVA"
     gene            complement(4631..5344)
                     /locus_tag="PKH60_02270"
     CDS             complement(4631..5344)
                     /locus_tag="PKH60_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNQ16905.1"
                     /translation="MALNPPTPFTARFEEKIVHNQRFVEFHFELQEPHRMPFKSGQYV
                     SLKVNDAGVRRSYSISSSPSVDHGFEFLIDVTPGGIGTQFLNSLQFGQTVEGLGPLGQ
                     FVLGDQSESAIVLLATGSGIAPLKSMLLDLVEQREDQRPITLYWGMRYAADLFWLDDF
                     QDIVEANTKVSFYPVISQPEPEWNLSTGHVTDLLEIHHFPQDAGYYICGSQATVQSIQ
                     QFLLAKSVQPQYIHHEKFF"
     gene            5430..6644
                     /locus_tag="PKH60_02275"
     CDS             5430..6644
                     /locus_tag="PKH60_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16906.1"
                     /translation="MSKINKEQSGDSLPEFQNPYKNPKWWENDQPPEGEAPLEEIVDE
                     LITEVTQRLLTEEGIVELAPSPQPITKPNRPEEKRTWQPDSSWRSPITLISSDQVFEE
                     LDEFLGLDFLPKISLELAEHVYSRIENSEPFYILDIDELSTSIESFRSSREKTDQSQR
                     ELKNAVDQFIFRKFKQIAEDALQLDVLSNYSLPGSRVLDIRAWQELEKLHGKVGILLI
                     FIALTGVEHMVSVSTQGISLDVSFYQSPETITKLLPLEGMVSNAAKSMRFSISNRPSS
                     TKRKIDLLPDKKNLGIFHDWWSIVRRHSGVINLDEVLKIEAFCVWDDPSFVRQNSNLQ
                     TSQTSGFFTSLQFSLPESYDPPLVVTRFGKRDFSLGSLNPEQILLCLGTLNQFENYQR
                     TTGKIQSESAQS"
     gene            complement(6620..7186)
                     /locus_tag="PKH60_02280"
     CDS             complement(6620..7186)
                     /locus_tag="PKH60_02280"
                     /inference="COORDINATES: protein motif:HMM:NF014041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HNQ16907.1"
                     /translation="MQKTPSYIFVGSTNPVKINAVRIATANHWPNVDITGFEVPSLVS
                     EQPRSDVETRQGAQNRAQAVLMLGKSETESQVTSLGVGLEGGVFTDSNGELWSTVWAS
                     VTDQSGQFWDANGARFKVPSQIAKLIEAGEEMGPALSQLFGGADIKRTNGAIGMVTNN
                     FVTRTEEYSAICKLAIGLWYGQDWADSL"
     gene            complement(7194..7580)
                     /locus_tag="PKH60_02285"
     CDS             complement(7194..7580)
                     /locus_tag="PKH60_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNQ16908.1"
                     /translation="MDLHELYPELVLEEVKNPQNRGKLSNADVSASEGVASCGDTATV
                     YVQVKSGKIQQMAWEAEGCAISRAGLSLLSVAVENQPIKKVEKWTIREMLSLLHLKQI
                     SPGREQCLMLGLRALQRALTQLSISK"
     gene            complement(7584..8867)
                     /locus_tag="PKH60_02290"
     CDS             complement(7584..8867)
                     /locus_tag="PKH60_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNQ16909.1"
                     /translation="MHNPSKVKADFPLFSQPENSNLVYLDNAATTQKPQVVIDAIANY
                     YKKSNANVHRGVHRLSDESTKAWLDARQTIADFFGAKSSELIITRNTTEAINGVAYGW
                     GDYQLKTGDVILSTQMEHHANLVPWQELCRRTGAELKIIPVLPDGRLDSDWYQAHLTK
                     KVKLVALQQVSNALGTLHPVNWYIDLAQTIGARVLIDAAQSAPHLPINFSKMRADFLV
                     FSGHKMLGPMGIGGLFVRDEIIKSGEMHPWLFGGGMIAQVSEVSAEYHSDPTERFTAG
                     TPDVASAVGLAAACTYLLNLNMRIVLEHDQSLVMYALGKLTENKKITLLGATTAAFVD
                     TTPVYELKGQFPQLQIDRVGSVSFLYKGVHPHDVAQILDSEGVAVRSGHHCCMPLHTK
                     FAWPGSIRASFSVYSTKQDVDRLVLALEKVATVFG"
     gene            complement(9127..9501)
                     /locus_tag="PKH60_02295"
     CDS             complement(9127..9501)
                     /locus_tag="PKH60_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16910.1"
                     /translation="MKKLKPEIYINQKDLEKWKIDNSRESESLLPLLFVYFLIISSLL
                     ILLFSDTDIEIPRIKINIEHNSLVLFYANILLLANSLLSGGFATMTDADILEAVGRVP
                     ITQIATDAPSHLMNSMILANPD"
     gene            complement(9504..9980)
                     /locus_tag="PKH60_02300"
     CDS             complement(9504..9980)
                     /locus_tag="PKH60_02300"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNQ16911.1"
                     /translation="MQEILLKQSRDILITETDSYVLYLLEPDIVVTIAGRFVASGTEQ
                     KQVKVTIHHRAPRTVAHTSLRGTSSDTAKLTFFGRIIIDPDCPQTNSFLEERILLLSP
                     NARGETVPELEILSDDVKCSHAATISTIDPEQLFYLQSRGLTLEQAEKMLVDSFLQ"
     gene            complement(10002..12263)
                     /locus_tag="PKH60_02305"
     CDS             complement(10002..12263)
                     /locus_tag="PKH60_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16912.1"
                     /translation="MQTLKAQITKDIKTISTKLVELKKVTKKMTLKSWMMLGGFAFVT
                     VGLLLALVVSRQSSENRSDASGQGLQYYFTPASAVLTQNTAGVLQPVQNVELKINAGR
                     PVVSYSFVMRLTDAAGAPVSDSVWQQVAFQPNPKFINHSSVLLSSTYPGAKLFAGTIG
                     QQPGGVTRLESTVTNGYLGAFTFTPLENQPDLRLSMFVIPGKPQANSVYQLGRLLTPP
                     AADGEEVITIPNQFNLHNPFLVVGSPTPTPQGAVVKKATLQYAVSNGAEWRQLDARFN
                     DFNNGGTVKSLIRLADIDPSWRVEGLSPAFRLRIKNAQGAYRQTTIGGQSTNVLVNFT
                     PNGTLAEGTFWVNWKVIEEAGVKYIQKPNTTDRLRFESGDTIEVTINLHRQQSGVSYT
                     CKETDELVVAPIGRPEEQQVMGPCVNDSLVTVTYGAGLAPGTCQCDLGVVMDSACAAA
                     TVATCTSQPVCACLPETPAATPTPLVSAYRNVNFMTGRVLQGADNASGANPSTIELVY
                     PIATTQSYLKLSVANTINATMHPYNSGWSPKIYAYTGGPTLPEPPITSSPTTGWVQRS
                     VTNMFAVNYEKVVNGYRYICMYDQSLWYQKVGETTKTRILNDFGQPFTCPNSIIEVTE
                     DYGGAPPPAPTATLTPTPSSCPVTQIPASQWVGPGNSSAACSATGMTFTWKDPYTNEY
                     EWRIYTVTASGVAGTVLGTVPASSTATKTFTYNPTGFNPRTDYKIKIRAIYRYQSGTE
                     CYQEAQISKLCQP"
     gene            complement(12270..13403)
                     /locus_tag="PKH60_02310"
     CDS             complement(12270..13403)
                     /locus_tag="PKH60_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16913.1"
                     /translation="MHKRSIRFSILLIILTLFVGFGVSFVAPKPVRADALGDFVARLT
                     LGDFIRGLFEGLFGGGRGGEAPYPTATPTSSPWFPTATPTRPIGPITATPTPNCGSVS
                     CSPPNSCGYYGGSTLRCVTPTPVMCGSSVCAPPKVCIYPAPGMSVCVDPSATPTPTRR
                     PTNTPVPLPPTHTPAPGAPTNTPAPTNTPVPNPTEPGQPPVGVPCYYNVKLVSKDSGT
                     ALPSALQTGVTEAGGCRTQGVMELVPSFYTQPSTATNNPEWPCTDADYVGQSCTQGIV
                     TGQFICMGGPQHEIKGCYNRTSDAYQRVSIVQVTQSGATATPTIAPGATHTPAPIHSS
                     FQNWFSEFITYVNTPGTPYSLSLSVLEDWRDNTPGPTPTPPAP"
     gene            complement(13493..14920)
                     /gene="sufB"
                     /locus_tag="PKH60_02315"
     CDS             complement(13493..14920)
                     /gene="sufB"
                     /locus_tag="PKH60_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNQ16914.1"
                     /translation="MSRFAQKPVATSQSPMVDTYQFGFSDSIDRYKYVAKPGLNEAVV
                     RTISEQKNEPKWMLAYRLAALKKFNSMQLPTWGGELGGIDFNQFHYYLRPTEAVVKSW
                     DDVPADVKQTFDKLGIPEAERSVLAGVKAQYDSEVVYGSLQNVWAKDGVVFLSMDEAL
                     QKYPDLVKEYFGTLIPSGDNKFAALNSAVWSGGSFVYVPPGVHVKLPLQTYFRINSPN
                     AGQFERTLIIIDEGASAHYVEGCTAPSFSSTALHSAVVEIFVKQGARCQYTTIQNWYK
                     NVYNLVTKRARVEAEGTMIWTDFNMGSKLTMKYPSCILAGKGARGETLSMALAGADQH
                     QDTGAKMIHLAPHTSSSVVSKSISKNGGRTSYRGLVHVGPKAEFSKNTVICDALLLDE
                     HSRSDTYPVDTVFQQQVAIQHEATVSKVGDEQLFYLMSRGVTEEMARKMIVRGFVDDL
                     IRQLPLEYAVEMNRLIDFEMEGSVG"
     gene            complement(14923..15411)
                     /locus_tag="PKH60_02320"
     CDS             complement(14923..15411)
                     /locus_tag="PKH60_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNQ16915.1"
                     /translation="MSQPHRLTDQVKILHKVAIVSNNQVLLLKRHGAAHSRPGKWDLP
                     GGNAEWPDNPAKPLLQKLHRTDVVREVNEETGITISESLIVSPIFFDTFFETEKQMYT
                     VVVGWLVKLDRHEEIVLSREHTEFRWAKQADIQNYDFGFAGGKDGFLVQIIEEAFTLI
                     GT"
     gene            complement(15424..16203)
                     /gene="sufC"
                     /locus_tag="PKH60_02325"
     CDS             complement(15424..16203)
                     /gene="sufC"
                     /locus_tag="PKH60_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNQ16916.1"
                     /translation="MPKVRKNTGLQIQSLAVEIEGKQVVDNFSLTIPPGECHVLMGPN
                     GSGKSSLAYALAGHPNYNVTSGSVLLDSNDVLALSPDQRAQLGLMLAFQYPVSVPGLS
                     VWQFLWQAYQQRFKTDIPKSGKKIVKAIDLIKSLEKPAKKVRMPAEMLKRGLNEGFSG
                     GEKKRLEVLQLLVLEPKFAILDETDSGLDIDAIKIIAKNIEQLTREYGVGCLLITHYQ
                     RLLRYVVPDRIHIMSAGRIVESGDKTLAEEVEITGYTKYQQ"
     gene            complement(16239..18467)
                     /locus_tag="PKH60_02330"
     CDS             complement(16239..18467)
                     /locus_tag="PKH60_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HNQ16917.1"
                     /translation="MKLFINFFVGLFALYALLLLSTEKASAYQIPPSVILPNSDTVVS
                     KNNIIAVPYIMNDWENCNPETLKRGTDNATDCWSHVPNVNPLPTTRSPFAAGSFGSHV
                     AFDWDTLNSAPGVYDWTSIDQYLQKASTMRVKDAQGKILPFKPVIITFTEYLQADSNP
                     HDGEKDLISYLPDYVKAALPQSELVSNLPGCDVQDMVPYHNSVFQQYYRQFITDALAH
                     LRQSPHAYVVQAYLMSGGLSHETTPSKNLADCRYGQTYRDQHQEAWRQFIIGSIAWTD
                     QVLKNGGSQHEIPAFLQAAASWPDERYLFFRTAYPLGIGFKMNSMAPSNANGISARPV
                     AIGLYDALVMTELTWPKGLEPVYPFRSPSSERCSERYCYSDARYQGTYWMDLTALNYQ
                     PDMFDRMKEHFLWTREFVEQFGLSWFNGLLENSYGKAANQSRVAWIAFAERFNDEDSY
                     PSTRPSPPVEMGVGDYYKAEFTERGDRDFYLYRLGEIEDSTLIRRQAGVIPMDQNNYA
                     LAQNSCYKTQAELPNQATKILTGQELRSSGKLASSQFAHPFSATALTTEGASGKTQIS
                     LVLEDQVQSIKTNSTSITVEYLDAGTDQFAVEFVNPQGQVQRETVTKTNTNRWKSYQI
                     IKTTGQISWQHRLPGGADLRLDCLCNSAAGDDIFSMVRLESQGPPMRVAPSNTLSFCG
                     EAVASCTASADVNQDGSVNLLDLTTIIAHFGNSGTSIYDVTCDQVVNLLDFSRVVSRM
                     QF"
BASE COUNT         5283 a         4174 c         3717 g         5358 t
ORIGIN      
        1 tttgcaggag catcactcgc gcaccacaga aaaaagagtg tctactgctt gtgaaaaatc
       61 aacgctcttc cataagccac ttccaattgt taccgactca acttcaagtg actgcagcag
      121 agctacttct tcttttttta ttcccccatc cacaatcagt tcatactgat atgatttttt
      181 ttgtcgcaaa ccttgtaact ctttaatttt atcaacaata gttgtcacaa aagcttgtcc
      241 ctgaaaacct gcctcgatgg ccatcacctg aacaaaatca agctccccca tgacgctggt
      301 ttcgatagat tcaatagggg taaagagatc caaagacaaa cccaccttcc agccagtctt
      361 ttttagctga tccacaaagg ttatctggct ggacattttt tctatttgag caatgacgga
      421 tcgaattggt aagttttcgc gatgctctaa caactcaaat acataatcta atggttcttc
      481 cgtcatcaaa tgaaaatcaa gttgtagctg tccatactca agatcggcat aatcagcagg
      541 tgtgagcgta agattgtcgg caaaccagcc atcaataata tcaatttgca acgcatcaaa
      601 tcgatcatca ctgttggcta attctatttg ggagattgct accgagcgac tctctgttaa
      661 tatggctgga attattttca cactaatatt gtagcagtta aagctctagt attagaaatc
      721 agtatacata tagtcacata ccgttttgat ctaaaaacac ccgaatatac ggtggcagac
      781 tgtgataaaa taatcaagaa atgctttatt ctcagtatag tagatacata aaaaaatcag
      841 acgctgaatt ttttgttaag caacaatatc gcctcattgc gattatgttg ctttttattg
      901 tgatcgcgtt tgggtttatc tattttacag ttttttccaa gatgatgacg atgtatgaag
      961 atttgggtca attacaaccg tttttggttc gtcaatttcc ttatggtttt acgatttttt
     1021 ctttagttgc agttctttcc tcagtgcgtt tttttacaaa agaagttgat gtcaaagaac
     1081 tgaataaaaa gttgtcaaaa tacaagcaaa acgaaatgat actctctaac gaggtactgg
     1141 atatgaaaaa tgccatattg gtaattgggg caacaatgtt atttgttttg tatatggttt
     1201 tttcaattat ttccccgata tacagtatca cccagtaatt ttttcagcca aaaccaatca
     1261 gccctcgaga tcgactaatt ttagaaatcc ctcctcacat attcaactat ggtttacact
     1321 aaagcgtgac ttctgaaggg gttatgcttt agtctaaact atcataagga aacgtatgtt
     1381 gcgcggatgt gactaccaat tgcactacag ttttctccaa tacacacatt actttgagta
     1441 ggttcgctca atggcttcga ttctttttaa gcggcgcacg tgtctctcca ctgcttcgaa
     1501 tggtgtattg agaaacattc ccacctgttg cttggcttct tcaaaggtaa caaaattgct
     1561 agcaatggct aaaatattga ggtcatcatg ggcgcgggcg tggtaaatat cggacaaatt
     1621 aattcccact gagcatcttg cgcccggcac tttgttggca gcaatcacca cgcccgcacc
     1681 agatccacaa attaaaattc cccgatgatc ttcatcggcc actacctgct ctgcaaccgc
     1741 atgagcaaat actgggaaat catcacctgg atccagcctt acattaccac aatccacaac
     1801 ggctagatgt tgtgtcacca gccattgctt taaccgctct ttcaaaccga acccacgatg
     1861 atccgcacca atataaatta tcacaaatta atcatacacg tatctaattg tcatttcact
     1921 tggaagaaag cgttagatga ggtagaatat gaagcttgat ttagaaatcc agattatgcc
     1981 aattaatagc gaacgctcca tgcatcaaag gccaggcgaa ggactaaaac accacctagc
     2041 cgatagagaa agactccgag actatcttct gtggttggtg tttgaaatac tcaaagagac
     2101 taccacgctc aaggcttcgg tagaaatttc cccctctcca tattttaaat ctagccgagt
     2161 gctaccaaac attacactat ctattgttgc agaagatcct gactcactgc gagaatgcat
     2221 tacaaaaatt acctcactta gtattcaaaa aggtttaaca ttactgagaa gaatgtctct
     2281 gggtgcaagt ggcaacgaca atggcaacgg agttgaaaaa aacaacgcga tatttgaact
     2341 ctcgctctat aataaccgtc accgaaccgt atcctagcct aaatcatccc caaatcggct
     2401 tttgcttcat cggtcatcat gtcttgagtc caaggtgggt caaaagtcag ggtaattgtt
     2461 acatcttcat cactatcaat tgtggatata gtagataaac tttggcgaac taacccatca
     2521 aaactatcgg ctaaagggca accgggcgtg gtcaatgtca tgagaatgtc tactttttgt
     2581 tgaccagttg cagttagatc cacagaaaca cgatatatga gtcccagact gacgatatct
     2641 atacccaatt cagggtctaa aacctgccga agagcctgcc aaattcgttt ttctaacttg
     2701 ttcacttggg tatttttttt cgctttcatc gcttcgtatt gtaacctgca aacacgatga
     2761 ttctctagag gaaataaaaa ccccgccgtt cacgaacggc ggggtaattt caaatcaatt
     2821 taaaaattat gcccttttaa gtactggcat gttggttctc ttggtttcca tcactgcata
     2881 accagcagga acggcatcga gtgaaccagc gcgaacatca cgagcgaaga aataaattct
     2941 ttgtactcga ccatttttaa gggtaacgtc cttcatgtgc aggtaataga cttgaccctt
     3001 agcgtttgtg tagctgaatg ccatagtgct ccttcttttt atttactact tttctacgat
     3061 agagtcttga ggttacctca agtgccctct attctttata ccatacctga ggaactctgc
     3121 aaggactaat cttgagttat ttagctgtat cggcttcaac tcgtcccttg agtaccacat
     3181 ttttcatttg ctcatcaagc gtttcacctc gagttacaaa cacatccagg ggcaactgat
     3241 ccacagataa tgcaccactg ccaactagcc cacctttatt tgcaacgagc ataaaaactc
     3301 gctttggggt ggtagaccca cttagttgca tccaaacgta ataatcaact gaaggatcac
     3361 cagattctgc aataacacta agtctgacct tatcattttc aatttcatac cgaacaacac
     3421 cagtgagagt ttcttcattt ggctcggatg actcagtttc atctaattga atggttttat
     3481 aatcacccac tccagaaatg gcatttttta attcttcttc acttaaattt tctatcgaaa
     3541 taccctcacc aacatctgga gttagtcgtg agcgatcaga gtaccaacgg aaaaccaaga
     3601 aaaatactat tactaccacc actgcgggaa aaatatacgg tgtaaactta ttcatactgt
     3661 accccttaaa ctatttagta cgagtgttgt tatcagctac tactgatcta acacattttt
     3721 cactgcactg agcagttccg gggcaggagt ctggattcca tttacaaaaa aggttggcgt
     3781 tgccataatg ttgagttctt tggcgagatc aacatctctc tgaactgctg agacaacttc
     3841 cgatgattcc attctctcaa gaaacgaggc gctgtcaata cctaattcag atgcatattc
     3901 actcaatgta gtctttaata gatcatatga actgaggtca gaccactctt cttgcttttc
     3961 aaaaagtaat tgtgcatact gccaaaattt tccttcactt tgagctacct gagcggcttg
     4021 ggctgccaat cgggcattgg gatggatttg atccaagggg aaattgcgaa aaaccaggcg
     4081 cacttgatct ggatattgtt ctaacaccga tttaaccatt ggctcacttg ctttacaggc
     4141 aggacattga aaatcagaaa attctacaat cgttactggg gcgtctgagg gaccagtgct
     4201 attcaaagca ccatcagcta gcaatgtagc atcaacttgt tcacctgaga ccgggcttgc
     4261 ttgaccagaa aaaagtaccg caatcccagc tattaatgca atactcacaa ccagcgtgcc
     4321 aatgagtaag ggtagatttt tcataggtaa cctttactta tgcgactggc caaacttgtt
     4381 gcccagattc tgtatctact acaattattg ccgaagtggg acaactctgc gcagccaata
     4441 attttgtttc atcatcatta ccatcttgac taatcacccg agcaatcccc tcctcatcca
     4501 aatcaaaaac acctggtgcg atagccacac aactagctgc actaatacac ttatcattaa
     4561 taacggagac ttggtattta ccaatttgtt tttgagcgac tgtgttttga tcttgagcca
     4621 tagaatattt ctaaaaaaac ttttcgtggt gaatatactg gggttgcacc gattttgcaa
     4681 gcaaaaattg ttgaatgctc tgcacagttg cttgggaacc acaaatatag taacccgcgt
     4741 cttgtggaaa atgatgaatt tccaacaaat cagtaacatg cccagtgctc aagttccact
     4801 ctggctcggg ctgagaaatg actggataaa atgaaacttt ggtatttgct tcaactatat
     4861 cctgaaaatc atccagccag aataagtcgg cggcatatct catcccccaa tacaaagtaa
     4921 taggtcgttg atcttcgcgt tgttcaacta ggtcaagcaa catgcttttg agtggtgcaa
     4981 tgccagaacc agtcgcgagt aacacaatgg ctgattccga ctggtctccc aaaacaaact
     5041 gccccaaagg accaagtccc tccacagttt gaccaaactg aagtgaatta agaaattgcg
     5101 ttccaattcc ccccggagtt acgtcaatta aaaattcaaa gccatggtct acgcttggcg
     5161 atgaacttat ggaataggaa cgcctcactc cagcgtcatt cacttttagt gacacatatt
     5221 ggcctgattt gaatggcatt cgatggggtt cttgcagttc gaaatggaac tcaacaaacc
     5281 gttgattgtg gacaattttc tcctcaaagc gcgcggtaaa aggtgtggga ggatttaaag
     5341 ccatggggac atactacaat gaaatgatac attttaaaac cgctacttca aatttactgt
     5401 ttggttgtaa aatataggct tacttaacta tgtctaaaat aaacaaagaa caatcaggcg
     5461 actcgttgcc agagtttcaa aatccatata aaaatcccaa gtggtgggaa aacgatcaac
     5521 cgccagaggg tgaggcacct ctagaagaaa tagttgatga actaattact gaagttactc
     5581 aacgtctgtt aactgaggaa ggaattgtag aactagcacc aagccctcaa ccaattacca
     5641 aaccaaatcg cccagaggaa aaacgcactt ggcaaccaga ctccagttgg aggtctccta
     5701 ttactttaat ttccagcgac caagtctttg aggagttgga cgaattctta ggtcttgatt
     5761 tcttaccaaa gatctccttg gaactcgccg agcatgttta ctcaagaata gaaaatagcg
     5821 aacctttcta tatccttgat atcgatgaac tcagtactag catcgagtct ttccgctcat
     5881 ccagagaaaa aactgatcaa tctcaaagag aacttaaaaa cgctgttgac cagtttatat
     5941 ttcgaaaatt taaacaaata gcagaagacg cacttcaact cgatgtactt tcaaactatt
     6001 cgttaccagg atcccgcgta ttagacattc gcgcttggca agaacttgaa aaacttcatg
     6061 ggaaagttgg catactacta atcttcatcg cactgaccgg cgttgagcac atggtttcag
     6121 tttcaactca ggggatatca ttagacgtaa gcttctatca atctccagag acgatcacta
     6181 agctactacc tctagaaggt atggtaagca atgcagcaaa aagcatgagg tttagcattt
     6241 cgaatcgtcc aagttcaaca aaaaggaaga ttgacctgct acctgacaaa aaaaatctag
     6301 gtatatttca tgactggtgg agtatcgttc gaagacattc tggagtcatc aacttggatg
     6361 aagtattaaa aattgaagct ttttgtgttt gggatgatcc atcatttgtg agacaaaatt
     6421 ctaatttaca aacctctcaa accagtggtt tttttacctc tctccagttt tcacttccag
     6481 aaagctatga tcccccactc gtcgttacta gattcggcaa aagagatttt tctctgggat
     6541 ctttaaatcc agaacaaatc ttactatgct tgggaactct taatcagttt gaaaactacc
     6601 aacgaaccac tgggaaaatt cagagtgaat cagcccagtc ttgaccatac cagagaccaa
     6661 tagcaagttt gcaaatagct gagtattctt cagtgcgggt aacaaaattg ttggtaacca
     6721 tgccgatggc gccgtttgtc cgtttgatat cggcgccacc aaaaagctga ctgagggcag
     6781 gtcccatttc ttcccccgct tcgatcagtt ttgcaatttg cgagggaact ttaaatcgtg
     6841 caccattcgc atcccagaat tgcccagatt gatctgtaac agacgcccag accgttgacc
     6901 acaactcacc attggaatcg gtaaatacac ctccctcaag tccaactccg agcgaggtta
     6961 cttgagattc tgtttcggat ttaccaagca ttagtacggc ttgagctcga ttttgagctc
     7021 cttgccgagt ttccacatcg cttctaggtt gttcggaaac caaagaaggc acttcaaatc
     7081 cagtaatatc aacatttggc caatgatttg ccgttgcaat tcgtactgcg ttaattttga
     7141 cagggttggt ggaacccacg aatatgtatg aaggtgtttt ttgcatctct tgattactta
     7201 ctaattgata gctgagtgag cgcgcgctgc agcgcgcgca atcccaacat cagacactgt
     7261 tctctgccag gagagatttg ctttagatgc aacaaggaaa gcatctccct gattgtccac
     7321 ttctccactt ttttaattgg ttgattttct actgctactg acaataaact gagccctgcc
     7381 ctgctaatag cacagccttc ggcctcccaa gccatctgtt gtatttttcc tgatttcact
     7441 tgaacataca cagtagcagt atcaccacaa ctcgctacgc cttccgatgc tgaaacatct
     7501 gcattagaca attttccccg attttgtggg tttttaacct cttcaagaac taattctggg
     7561 taaagttcat gcaaatccat acattatcca aatacggtgg ctactttttc caaagccaag
     7621 acaaggcggt caacatcttg ttttgttgaa tacactgaaa agcttgccct aattgaacca
     7681 ggccaggcaa attttgtatg taacggcata caacaatggt gtcctgagcg aaccgcaact
     7741 ccctcactat ctagaatttg cgccacatca tgtggatgta cccctttgta taaaaagctg
     7801 accgatccaa ctcgatcaat ttgtagttgt ggaaattgtc ctttgagttc gtatacagga
     7861 gtagtatcca caaaagctgc tgtagtcgct ccgagcaatg taattttttt gttctctgta
     7921 agtttaccca aggcatacat aactaaagat tgatcgtgtt ccaaaactat tctcatgttg
     7981 agattcagta agtaggtaca agcggctgcc aaaccgactg ctgaggctac atctggtgta
     8041 cctgcagtaa atcgttcagt cggatcagaa tgatactctg ctgacacttc agaaacttgc
     8101 gcgatcattc ctccaccaaa aagccaaggg tgcatttcac cagattttat aatctcatcg
     8161 cgcacaaaca agccaccaat gcccattggg ccaagcattt tgtggccaga aaataccaaa
     8221 aagtcagctc gcattttgga aaaattaatc ggcaaatgcg gcgctgactg cgccgcatca
     8281 atcaagactc gcgctcctat cgtctgagcc agatctatat accaattaac tggatgcaac
     8341 gtccccaaag cattagaaac ctgttggagc gcgactagtt taaccttctt agttagatgt
     8401 gcttggtacc aatcgctgtc tagtctgcca tcaggaagaa ctgggattat ttttaactca
     8461 gcaccggttc tccgacaaag ttcttgccac ggtactagat tggcatgatg ctccatctgt
     8521 gtagacagaa ttacgtctcc agtcttcagc tgataatcac cccagccata cgccacacca
     8581 ttgattgctt cagtagtatt gcgcgtaata atcaactcgg aagattttgc accaaaaaaa
     8641 tcggcgattg tttggcgtgc atccaaccaa gcttttgtgc tttcatcaga taagcggtgt
     8701 acaccacgat gaacgtttgc gttacttttt ttgtaatagt tagcaattgc atcaattacc
     8761 acctgtggtt tttgggtggt cgcagcgtta tccaaataaa ccaagttgct attttctggt
     8821 tgactaaaga gcggaaaatc ggctttaacc ttggatggat tgtgcatata ccatgtattg
     8881 tacacgctgt ctacttcgac aagctcagta tgacagcgac tggtgactag agctcaggat
     8941 gacttcagtg tgacaacctg aaaaagttaa agtgggttga tctgtatagc gacaaacttg
     9001 tctgtaaaat gcaaatggtt gttactgcta cttaacatgg gtttcatttt gatggtttac
     9061 actaaaccat ggctccagat agggttatgc tttagtgtaa accattgctg tatatgtgag
     9121 gtgaaattaa tcgggattgg ccaaaatcat cgagttcatt aaatgcgacg gagcgtctgt
     9181 ggctatttga gttattggta cccgcccaac tgcctctaaa atgtcggcgt ctgtcatggt
     9241 tgcgaatcca ccagatagaa gtgagtttgc cagtagtaat atatttgcat aaaacaacac
     9301 aagtgaatta tgttcgatat taattttaat cctcggaatt tcaatgtctg tatctgaaaa
     9361 tagtaatata agtaggctac taataataag aaagtagaca aacaataaag gtaaaagtga
     9421 ttcagattcc ctagaattat cgattttcca cttctctaag tctttttgat ttatgtaaat
     9481 ttctggctta agttttttca tgcttactgt aaaaaactat ctaccagcat tttttctgcc
     9541 tgttcgagag tcagccctct gctctgtaaa taaaacaatt gttctggatc gatagtgctt
     9601 atggtcgccg catgtgaaca cttgacgtcg tcggagagaa tttctaattc tggcacagtt
     9661 tctcctctgg cattgggcga aagtaacaaa atgcgttctt ctaaaaatga atttgtttgc
     9721 ggacaatcgg ggtcgataat gattctgcca aaaaaggtca gcttggctgt gtcagaactc
     9781 gtcccacgca aacttgtatg cgccactgtc cgtggagcgc ggtgatgaat ggtaactttt
     9841 acttgtttct gttcagtccc agaggcaaca aaccgtccag cgatcgttac cacgatatcg
     9901 ggttctaaca gatacaacac atatgaatct gtttcagtaa tcaggatatc tcttgattgt
     9961 ttgagtagga tctcttgcaa aacgggtgca gtatatcaaa actatggctg acagagttta
    10021 ctgatttgcg cttcttggta gcattctgtt cccgactggt atctgtaaat tgctcggatt
    10081 ttaatcttat aatcagttcg tggattaaac ccagttgggt tataggtaaa ggtttttgta
    10141 gcagtacttg atgcgggcac tgtccccagt accgtaccag ccacaccact ggcagttacc
    10201 gtatatattc tccactcata ttcattggta taaggatctt tccaagtaaa cgtcattccc
    10261 gtggctgaac aagctgcaga agagttgcca ggaccgaccc attgcgaagc aggtatttgg
    10321 gttacaggac aacttgaggg cgtaggtgtt aaggttgcag ttggcgcagg aggaggagct
    10381 ccaccgtaat cttcagttac ttcaattatt gagtttggac aggtaaatgg ctgaccaaaa
    10441 tcgttgagga tcctcgtttt ggttgtttca ccgacttttt ggtaccacag actttggtca
    10501 tacatacaga tataccgata gccattgacc accttctcat aatttacggc aaacatgttt
    10561 gtgacacttc tctgcaccca acctgtagtg ggactagagg tgattggggg ttccggaagc
    10621 gttggtcctc ctgtatacgc ataaattttc ggcgaccagc cgctattgta aggatgcatc
    10681 gttgcattga tggtgttagc aaccgacagt tttaagtatg actgagtggt agcaattgga
    10741 taaacgagct ctatcgttga tggattcgct ccggaagcat tgtctgctcc ctgcaatact
    10801 cgaccagtca taaaattcac atttctgtag gctgacacta gcggcgttgg cgtggccgct
    10861 ggtgtttctg gcaaacaggc acaaacaggc tgtgaggtac atgtagccac agtagcagct
    10921 gcacaagcac tatccattac tactcccaaa tcacactgac aggttccagg agcaagaccg
    10981 gcaccatacg taactgtgac cagcgaatca ttgacacaag gacccatgac ctgctgctct
    11041 tctggcctac caattggagc tacaactaat tcatctgttt ctttacaggt ataactaact
    11101 ccactttgtt gtcgatgcaa gttaatagtt acttcaatcg tatcgccaga ttcaaaccta
    11161 agtctatccg ttgtatttgg tttctgaata tactttactc cagcttcctc gattaccttc
    11221 cagttaaccc aaaaggtacc ttctgcaagt gtcccatttg gggtgaaatt cacgaggaca
    11281 ttggtgcttt gtcctcctat ggtcgtttgt cgatacgcac cctgagcatt cttaattctg
    11341 agtcggaaag cgggtgaaag accctcaact ctccaagatg gatcaatatc agccaatcta
    11401 atgagcgact tgacggttcc tccattatta aagtcattaa accgagcatc aagttgtcgc
    11461 cactctgcac cattagaaac cgcgtactga agtgtagctt ttttaacaac tgctccctgt
    11521 ggagttggcg ttggggaccc aaccactaag aaaggattgt gcaaattaaa ttggtttggt
    11581 atggtgatta cctcttcccc atccgcagct ggaggggtta gtaatctacc caattgatat
    11641 acagaatttg cttggggttt gccagggatg acaaacatag aaagcctcaa atcgggctga
    11701 ttctcaagag gagtaaatgt gaaagcacct aaatacccat ttgtaacagt gctttctagt
    11761 ctagttacac cgccgggttg ttgcccaatg gtgccagcaa atagttttgc acctgggtag
    11821 gttgagctca acagaacact agaatggtta ataaattttg gattcggctg aaatgcaact
    11881 tgttgccaga ctgagtcgga aactggagca cccgcggcat cggttagtct catcacaaaa
    11941 ctataactaa ccacaggtct tcctgcatta atttttaact caacattctg aactggctgc
    12001 aaaactccgg cggtattttg cgtgagcaca gctgaggctg gagtaaagta atattgtaac
    12061 ccttgcccag aggcatccga acgattttca gatgattgac gtgataccac tagagcaaga
    12121 agtaatccaa ctgttacgaa agcaaaacca ccaagcatca tccatgactt gagagtcatc
    12181 ttttttgtga ctttctttaa ctcaacaagc tttgttgaaa ttgtttttat gtctttggtt
    12241 atttgagctt ttagtgtttg catatcttat tatggtgccg gaggagtagg tgtaggtccc
    12301 ggggtattat ctcgccaatc ttctagtacg ctaagtgaaa gcgagtaagg tgttccagga
    12361 gtgtttacat aggtgataaa ttcactgaac caattctgaa atgagctatg aatgggcgct
    12421 ggagtgtgag ttgcccctgg tgctattgtt ggagttgcgg tagcaccaga ttgagtcact
    12481 tgaacaatac tcacccgctg ataggcatca cttgtgcggt tgtaacagcc cttaatttca
    12541 tgctgtggcc cacccataca gatgaattga ccggtgacaa tgccttgagt gcaactttga
    12601 ccaacataat cggcatcagt acatggccat tcaggattat ttgttgcagt ggaaggttgt
    12661 gtatagaatg acggcaccag ctccatgacg ccctgagttc tgcacccacc agcttcagtt
    12721 acacccgttt gtagtgctga cggtaaagct gttccagaat ccttacttac caatttaacg
    12781 ttgtagtagc atggcacacc aacaggtggt tgcccaggtt cagtaggatt tggtacaggt
    12841 gtattggttg gagctggagt attagtaggt gctcctggtg ccggcgtatg tgttggaggt
    12901 agtggaactg gagtatttgt tggtctgcgc gttggagttg gagtggcaga aggatcaaca
    12961 cagacagaca tacccggcgc tggatatatg caaaccttcg gaggagcaca aactgatgat
    13021 ccgcacatta ctggtgttgg agtaacacac ctgagtgttg atccaccata gtaaccacag
    13081 ctgttgggtg gactacatga tacagatcca caatttggag ttggtgtggc agtgattgga
    13141 ccaattggtc tagttggtgt tgcggtagga aaccaaggac ttgaggttgg tgtggcagtt
    13201 gggtatggag cttctccccc cctaccgcca ccaaacaacc cctcaaacag tccacgaata
    13261 aaatcgccta gtgttaatcg tgcgacaaaa tcaccaagcg catctgctct gacgggtttt
    13321 ggcgcaacga aagaaacacc aaaaccaaca aacaaagtca agataatgag taaaattgaa
    13381 aagcggattg atcgtttatg cataaacaac acttcttgta atcaagaata gcacctggaa
    13441 aatcagggtg tcaatgagga atttattgtt taccaaatgt atgtttactg cactaaccca
    13501 cactaccttc catttcaaaa tctatcagcc gattcatttc cactgcgtat tccagcggta
    13561 attgtcgtat caagtcatca acaaaaccac gtacgatcat tttgcgcgcc atttcttctg
    13621 tgacgccccg gctcatgaga taaaacaatt gttcatcccc cactttggag acagtggctt
    13681 catgttgaat tgccacttgc tgttggaaaa cagtatctac tgggtaggtg tcggagcgcg
    13741 agtgctcatc gagcaggagc gcgtcgcaaa taactgtatt tttactaaat tccgcctttg
    13801 gccccacatg tactaagcct cgataactcg tcctaccacc atttttacta attgacttgc
    13861 tcactacaga agaactggtg tgtggcgcta aatgaatcat ttttgcaccc gtatcttgat
    13921 gttgatcagc tccagccaaa gccatgctga gcgtctctcc tctagcccct ttccccgcta
    13981 aaatacagct aggatatttc atcgtcaatt tactgcccat gttgaaatca gtccaaatca
    14041 tggtaccttc ggcttcgacg cgcgcgcgct tagttactag gttgtacacg tttttgtacc
    14101 agttctgaat tgttgtgtac tggcagcgcg cgccctgttt cacaaatatt tctactactg
    14161 ctgaatgcaa tgccgtacta gaaaaacttg gagccgtaca tccctctaca taatgcgcac
    14221 tcgcaccttc atcaataata atcagtgttc gttcaaactg acctgcattg ggcgagttaa
    14281 ttctaaaata cgtttgtaac ggtaatttaa catgaacacc cggtggcacg tacacaaacg
    14341 acccaccaga ccagaccgcc gaattgagcg ctgcaaattt attgtctcca ctgggaatca
    14401 aggtaccaaa gtattcttta actaaatctg gatacttttg cagcgcttca tccatcgata
    14461 aaaaaactac gccatctttt gcccaaacgt tttggagcga tccatagact acctcgctgt
    14521 catactgtgc tttaacccca gctaaaaccg agcgctctgc ctcaggaata cctaatttgt
    14581 caaatgtttg ctttacgtct gcaggaacgt catcccagga tttgaccacc gcttcagttg
    14641 gtcgaagata gtagtgaaat tgattaaagt caatgccgcc cagctctcca ccccatgttg
    14701 gcaattgcat actgttaaat tttttcagcg ctgccagtcg atatgccaac atccacttgg
    14761 gttcattttt ttgttctgaa attgttctga ctaccgcttc atttaatcca ggcttcgcta
    14821 catacttgta acgatcgatt gaatctgaaa aaccaaattg ataagtatcc accattggcg
    14881 actgtgaggt cgcaactggt ttttgagcaa atctcgacat gattatgtac ctatcagcgt
    14941 aaaagcctct tcaatgatct gtaccaaaaa gccatctttt ccaccagcaa aaccaaagtc
    15001 ataattttga atatcagctt gtttagccca tctaaattca gtatgttctc tgcttaaaac
    15061 aatttcttca tgtctatcaa gcttcacaag ccaccccact accacggtat acatttgttt
    15121 ttctgtttca aaaaatgtgt cgaaaaaaat tggtgaaaca atcaaacttt ccgaaattgt
    15181 gatacccgtt tcctcgttaa cttccctaac aacatcagtt ctatgaagct tttgtaacaa
    15241 aggtttagct ggattatctg gccactcagc attcccacca ggcaaatccc acttaccagg
    15301 acgagagtga gctgccccat gtcgtttgag caataaaacc tgattatttg aaacaattgc
    15361 cactttgtga agaattttga cttggtctgt gagtcgatgt ggttgagaca tagttgtact
    15421 taattattgt tgatacttgg tatagccagt tatttcaact tcctctgcca aggttttgtc
    15481 gccagattca acaattctac cagctgacat gatgtgaatc cgatcaggca ccacatacct
    15541 gagcagtcgt tgataatgag taatcaacaa acatccaacc ccatattcac gcgtgagttg
    15601 ttcaatgttt ttcgcaataa tcttaattgc gtcaatatct aaaccagagt cagtttcatc
    15661 caaaatggca aattttggct caagaactag gagttgcaat acttctaacc gttttttttc
    15721 gccaccacta aaaccttcat tcagacctcg cttgagcatc tctgcaggca tacgaacttt
    15781 tttggcaggt ttttcaagtg attttattaa atctatcgct ttgacaattt ttttaccact
    15841 ttttggtatg tcagttttga atctctgttg gtatgcctgc cataaaaatt gccaaacaga
    15901 aagtccaggc accgaaacag ggtattgaaa cgccaacatg agtccgagct gcgctctttg
    15961 atctggagat aacgctaata cgtcattaga atcgagtaaa actgaaccag aggttacgtt
    16021 atagttcggg tgaccagcta aagcatacgc cagcgaactt ttcccagacc cattgggacc
    16081 catcagcaca tgacactctc ctggtggaat agtgagtgaa aaattatcaa caacttgctt
    16141 tccttcgatt tcaacagcaa gagactgaat ttgaagtccg gtattttttc ttacctttgg
    16201 cataccagca ggtatcgtac cacatctggc acctagcact agaactgcat tctggatact
    16261 acccgactaa aatctagtaa attgacaact tggtcgcaag ttacatcata gattgatgta
    16321 cctgagttgc caaaatgagc aataattgta gttaaatcga gtagattaac agatccatcc
    16381 tgattgacat ctgcggacgc agtacagctt gcaactgctt caccacaaaa actcaaagta
    16441 ttacttggag ctacacgcat tggtggacct tgcgactcaa gcctcaccat gctgaaaata
    16501 tcgtcaccag ctgctgaatt acacagacaa tctaagcgta gatctgctcc cccaggtaat
    16561 cgatgttgcc aagatatttg accggtagtt tttattattt gataggattt ccagcgattg
    16621 gtattggttt ttgtaactgt ctcgcgctga acttgaccct ggggattgac aaattccacc
    16681 gcaaattgat ctgtgccagc atctaagtat tcaaccgtga tactcgttga attagtttta
    16741 atactttgta cctgatcttc tagtactaat gaaatttgag ttttcccact ggctccttcg
    16801 gtagttaacg cagttgcaga aaatgggtgg gcaaattggc tactcgccaa tttgccagaa
    16861 ctacgtaatt cctgaccagt caaaatctta gtcgcttgat taggcaattc tgcctgcgtt
    16921 ttataacaac tattttgcgc taacgcatag ttgttttgat ccatcgggat cacacctgcc
    16981 tgtcttctga tcagagtcga gtcctcgatt tctcccaatc gatacaaata aaaatctcgg
    17041 tcacctctct cggtaaattc cgctttgtaa tagtcgccaa ctcccatctc aaccggcggt
    17101 gacggtcgag tgctgggata tgagtcctca tcattaaaac gctcagcaaa agcaatccag
    17161 gctacccgac tttggttagc ggccttcccg tatgaatttt cgagtaaacc gttgaaccaa
    17221 cttagaccaa attgttctac gaattctctt gtccaaagaa agtgctcttt cattcggtca
    17281 aacatatctg gctgataatt gagtgctgtc aaatccatcc aataggtacc ttgatatcga
    17341 gcgtctgaat aacagtagcg ttcagagcaa cgttctgagc ttggcgaccg aaatggataa
    17401 acaggctcaa gacctttcgg ccaagttaac tcagtcataa ccaacgcatc atataaacca
    17461 atcgcgactg gacgagcgct gataccattg gcatttgagg gagccatact attcattttg
    17521 aaaccaatac cgagtggata ggcagtgcga aaaaatagat atcgttcatc cggccaagat
    17581 gccgctgctt gtaaaaatgc tggtatttca tgttgtgagc ctccattttt taacacttga
    17641 tccgtccagg caatactacc tataatgaat tgtctccagg cttcctgatg ctgatctcta
    17701 tatgtttgcc catatctgca atcggccaaa tttttacttg gtgttgtttc gtgactcaaa
    17761 ccgccagaca tcaaataggc ctgcaccaca taggcatgcg gtgactgtcg caggtgtgcc
    17821 agcgcgtcag taataaactg cctatagtac tgttggaaaa cagaattgtg atacggcacc
    17881 atatcctgaa catcacaacc aggcagatta ctgactaatt ccgactgagg caatgctgcc
    17941 ttgacgtagt ctggcagata tgagattaag tctttttctc catcgtgagg attgctgtcg
    18001 gcttggaggt actcagtaaa ggtaatgatt acaggcttga aaggtaaaat ctttccctgc
    18061 gcatctttca cacgcattgt agaagccttt tgcaggtatt ggtcaattga cgtccaatca
    18121 taaacacctg gggcagaatt gagcgtatcc caatcaaagg caacatgact accaaatgaa
    18181 cctgcagcaa agggagatct agttgtaggt agagggttta cattgggtac atgagaccaa
    18241 caatcggttg cgttatctgt accccgtttt aacgtttctg gattacaatt ctcccaatca
    18301 ttcatgatat atggaacagc aataatgtta tttttactca ccaccgtatc gctattgggc
    18361 aagatcactg agggaggtat ttggtacgcg gaagcctttt cagttgaaag aagcaataag
    18421 gcataaagtg caaacaatcc aacaaaaaaa ttaataaata atttcactct ttcagtctat
    18481 caaaaagacc atcggaggaa cagttgcgat aaatccttca gatcaatagc at
//
LOCUS       DAOQZP010000021        18285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_3446_length_18285_cov_5.172628, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000021 DAOQZP010000000
VERSION     DAOQZP010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 18285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18285
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3446_length_18285_cov_5.172628"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..226
                     /locus_tag="PKH60_02335"
     CDS             47..226
                     /locus_tag="PKH60_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16918.1"
                     /translation="MRVKELVSKLPELGKALERIFKDGAVFDSRYPVISYTGDLQVEK
                     DQQSAEIDDTKISQT"
     gene            complement(223..2139)
                     /locus_tag="PKH60_02340"
     CDS             complement(223..2139)
                     /locus_tag="PKH60_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNQ16919.1"
                     /translation="MEAATLAGVFRSEATVDDIMNYIKGPDFPTGGIMYDFAAIKEGY
                     KTGKGRVVVRAKASIEEGPKGFQIIITELPYQVNKARLLMKIADLVRDKKILGIRDLN
                     DDSDRVGMQITIDLKRDARPKVVLNKLFKYSELQSSFPMNMVALNSEGIPQLMNIKQI
                     ITEFIAHRQLVVTKRCQYELAEARDRAHILEGLLIALNNLDEVIDTIRKSADTDAAKI
                     ALMTKFGLSEIQAQAILEMQLKRLAALERQKIEDEYKQLQEVIDRLLLLLQNPVEILA
                     VVKQETHDLKALYGDERKTQLIKGKIGEFSEEDLVPNEPTVITLTESGYIKRLEPGAF
                     RSQSRGGKGSVGLKMRDEDIVKTLLVAKTHDTLLLFTNQGKVFKMRAFDIPQANRQAK
                     GTAIVNLLSLQPDETVQSLLIIDEEADKEKFITLTTKKGLVKKSAVALYQNIRQTGII
                     AIILNDDDKLVRSTLTGGSDDILMISHQGKVIRFSEDEVKTSNRDTKGVKGITLKSND
                     YVVGVETVTPEMAKLAGSSDEPALLVITERGLGKRTVLSQYPSQKRTGMGVKVSDITT
                     KTGPVASARLVEADHKDVVITTREGITIKLPMTKKSIPILTRPTQGVILMRLKGDDYV
                     AAVALTFKQMDDED"
     gene            complement(2227..2802)
                     /locus_tag="PKH60_02345"
                     /pseudo
     CDS             complement(2227..2802)
                     /locus_tag="PKH60_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440942.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
     gene            complement(2970..3446)
                     /locus_tag="PKH60_02350"
     CDS             complement(2970..3446)
                     /locus_tag="PKH60_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNQ16920.1"
                     /translation="MSDVLVKIEIPEGSRVKYELDEETGEMMVDRIVPTAMDYPVNYG
                     LIEGTKGQDGDALDALVFLSAPVIPGAVVKCRVIGMLEMEDEAGIDHKLVCVPAKMKI
                     DPICGTWESLDDIPEMKKRQIKHFFEHYKDLEEGKWVKLKDWADATTALAVLEKSK"
     gene            complement(3459..5477)
                     /locus_tag="PKH60_02355"
     CDS             complement(3459..5477)
                     /locus_tag="PKH60_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HNQ16921.1"
                     /translation="MTNTDNHYNAREIKVLEGLEPVRKRPGMYIGSTDTKGLHHLAKE
                     ILDNSVDEAIAGFGGTIQLFRSPTTAEEIKLAGIPEVGSESITVIDNGRGIPVDMHES
                     GVSALEVVLTKLHAGGKFEETAYSASGGLHGIGSSAVNALSSLLQVVIKRDKKYHYQA
                     YVRGVPQKSVAVIDEKKVQELFPEQSARLIQKETGTLLQFIPDPTIFSTTQFSPKTLV
                     QIITDRAYLMAGIYFELHDQVEKQTQHYYFEGGIRSLVEHINMNRKPLHPVFHSEKTW
                     TDPETGKKIVVETALQYNDSFNEKLESYVNVIHTPDGGTHVNGFRMALGKVVRDYASD
                     NKLMKEKEIFSAEDLREGLAAVIFVKMPSNDLQFESQTKAKLNNSEAQPAVYQVFKDD
                     FATFLEENPAAARAIVGKVMLSTRARLAARAAKDAVVRKGLLEGSTLPGKLADCQSKD
                     AAESEIYIVEGDSAGGSAKQGRDRRFQAIFPLRGKILNTERARLDKIVAFEELKNLVI
                     ALGSGIGDTFSVKKLRYHRIILMNDADVDGEHITTLGLTFFFRHLREIVQQGYLYIAL
                     PPLYKITTGKDVHYTYSEEERETLVAKLKSENANVKLGIQRYKGLGEMNPEQLWDTTM
                     NPATRMLKRVTIDDADKADSIFSTLMGDDVPPRKKFIQTHAQLAELDV"
     gene            complement(5527..6240)
                     /locus_tag="PKH60_02360"
     CDS             complement(5527..6240)
                     /locus_tag="PKH60_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16922.1"
                     /translation="MTDTTMATIFSAYKQPLLLVLPVEADQQNSIAGILAQYSPYTNA
                     LLHPAVQWINPDLESLGIDTARQITTELSFSPLDNQIRVFVLLGFENATTEAQNALLK
                     LLESQTTQTQFWLVTQILSRVLPTIQSRCSIVKVPLEHLVLKEQSSAEVANLFSEISQ
                     ASIAECISLASKYPDRTDALNLITSLLRWLTQQTNFIPSSTQLRCLQLAFSRLEQNAN
                     VKLALESAFFGLSGAKDHY"
     gene            6292..>7777
                     /locus_tag="PKH60_02365"
     CDS             6292..>7777
                     /locus_tag="PKH60_02365"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNQ16923.1"
                     /translation="MTEPANAYRSSITPPGLMLPQVNAGQVPQNTTTLVGSGMGSVIP
                     PAIASSSAAKPKSIDELSVIQIPSQEPTVISSNNTPTQQMPSNNSPQLATSSGIDSGT
                     EDIPIQFIGDAPTEAEIDTAQASITPSILPSEVPVIPIDSGPPTSVVQNATSPSEPIL
                     VAPEVVPNPISEPVVTSSKPDVQETQSVVEPPVKASESVDNQAKKTKSTAPLSLLDIL
                     VNQQLLTTEQANEVLTQNIQTGLNIEHLIEKNNLVSEEDLVKARSEFYNIPYVDLNEV
                     GVYPEALAQVPETVARNYWMLPFALNKDKNTLSVAMRNPLDVQAVDFVEHKTGHKVIA
                     HFASASEIERLIAEQYSQSLSTEVTQALEQTKQVQQQKESQQDFGELSGGLIRQAPIT
                     KIVQTILSFSLKARASDIHIEPQENRTRVRYRIDGILQEKLILPKSVHQAVVSRIKIL
                     SGLKIDERRLPQDGRFDFSENNQQVDLRISTLPTIHGEKVVMRLL"
     assembly_gap    7778..7877
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7878..8804
                     /locus_tag="PKH60_02370"
     CDS             <7878..8804
                     /locus_tag="PKH60_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNQ16924.1"
                     /translation="RISTLPTIHGEKVVMRLLNKNANVPTLEEIGLSGLALRNVQDAI
                     KVPHGIVLVTGPTGSGKTTTLYSVLHTINTPRVNIMTLEDPVEYQMPGINQVQINPQA
                     GLTFASGLRSFLRQDPNIIMVGEIRDSETAGLAVQASLTGHLVFATLHTSSAAGALPR
                     LMDMGAEPFLLASSMTCVLAQRIVRIINPDYKEAYKPEPAVIENIKTVLGDNFARWLQ
                     QNQKTEAEVVLMRPKKDRPEAEPDYKGRVAIFEVMKISEEIAKLIMARKAASEMEKVS
                     LKDGMLFMKQDGYLKVLDGVTTIEEVLRVAEI"
     gene            8823..9890
                     /locus_tag="PKH60_02375"
     CDS             8823..9890
                     /locus_tag="PKH60_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNQ16925.1"
                     /translation="MSTTITIHDLMQKVVDLQGSDLHLVCGNPAILRVDGELRRVEMP
                     LITPAQNKELIFSILTQEQQDYVKVNKELDFGYQYKEKARFRVNVYHASGALALAARL
                     IPEKIKTIEELDLLPILHDFTLYPQGLVLLTGPTGEGKSTTLAALIDEINHKRAEHIL
                     TIEDPIEFIYQPDKSIISQREVNQDTHGWDIALRSALREDPDVVLIGEMRDHETIASA
                     ITIAETGHLVFATLHTNTASESMDRIIDVFPAAQQPQIRQQLAGVIRAVVSQRLLPKK
                     GGGRVAALEVMIANAAVRNLIRESKAFQIESIIQNSSEQGMFLFEKHLFMLVQQGKIT
                     AETARNRAFRPDEIERMLAKE"
     gene            9897..11099
                     /locus_tag="PKH60_02380"
     CDS             9897..11099
                     /locus_tag="PKH60_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNQ16926.1"
                     /translation="MPFFSYSAKNKLGEVVKGKAEASSMVDAAALLDERGLLVINLKP
                     VGMEGLGSLLQGFMGVSQNDVVNFTRQLSTMISAGLSISKALGVLIQQSTPGFSQLVA
                     NLLHEVEGGASFSKALSSHPKVFSRLYVQLVKAGEVGGILDDILSRLAHNLEKSKEFR
                     AKTKGAMIYPVIVVLAMLVVGFIMMTFVLPKLTAMYSDFGVTLPLPTRILIGVSNIFA
                     TRWYLILAGIGGGIFLFNRWYKTEAGQRVIAGLLLKLPIFGNMLQKIMITDFVRTLSL
                     LLGSGVTLLQALDVVIEGVENVIYREAFTEARNQVEKGGLLSESIARHEMIPLILSQM
                     VAVGEETGKLDEVLQKLSTYFEQETEQAVKNMTTAIEPIIMVILAVAVGGMVIAIVMP
                     IYSLTSAF"
     gene            11189..11827
                     /locus_tag="PKH60_02385"
     CDS             11189..11827
                     /locus_tag="PKH60_02385"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ16927.1"
                     /translation="MKKALLARLKSGGFTMIELLIVISILGILAVMVLAAINPLEQIN
                     RGRDTAKRGDAEQLLGALERYNAFNGFFPWQEDANDITGLALTGQLVNSGWVETNSNC
                     QILERLSTAVTAGCVGTQELKDSYITRVDDDGGGSTGTVEARGLYVYYEAAAGDNVYV
                     CFSPQSNAFVNEATLRCDGTLPDDLAPATGICVDDVTANLAGETGIDMICLP"
     gene            11969..12793
                     /locus_tag="PKH60_02390"
     CDS             11969..12793
                     /locus_tag="PKH60_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNQ16928.1"
                     /translation="MFIFQLMLSLLIFALGVCIGSFLHVLVYRTVEDKEDWKTGRSRC
                     DHCSYIIKWYDNIPLLSFVLLGGKCRKCHKPIAISHVAMEVMVGALFVWWYWAGSVFF
                     LLVDQPFTVLQPLFWLLIGTLSLVIFVADMKYFIIPDWAVAIMALATLVYRVVLVQGK
                     IMQQTDFISMILASIGVGAFFWLLRVVTKGKGMGFGDVKLAPVLALLVGWPQVLVALM
                     VSVCSGALIGSLLLLLGKHKLKQPIPFGPFLLLGSAVALVWGDAIWGWYWTQLLGM"
     gene            12806..13765
                     /locus_tag="PKH60_02395"
     CDS             12806..13765
                     /locus_tag="PKH60_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16929.1"
                     /translation="MRFFRYSFIFIVIFTSIAIGAFLAIQQWVLFQAYNQLLADASLV
                     QRSKHVGEYYEYCTELGAENVDDGPLYTPQLRFLSDTEYVLEVVCDQYEFVPKQFGEK
                     KLPLFASKKPGQSGLVFAEEGVWSISIQAVPADLEIWLKKIALLENLVFRTQTISVED
                     GSMVKSLSVSPDAIEPITTCEGYGFSCCDPVRLLGVGDQRPGSDCSDDCYSSCHQRPI
                     ILSFNAEPGFDRYQRILQASQADLISFNFVIDSLLPEESQVTLEFGDGTSWIGIATET
                     MTNHQYSCNQPVCNYQATLKVVDAYGTVSASTSATKMNIAIHQ"
     gene            13811..14230
                     /locus_tag="PKH60_02400"
     CDS             13811..14230
                     /locus_tag="PKH60_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNQ16930.1"
                     /translation="MKKNGFTLIEILIVVAIIGILATMGIVAFQTSLRRSRDATRIAE
                     IKALQNAAEQFYADNASEYPTVDGCAQLAPYLQGFQDYNDPRGVAYECDFTPAGAVTW
                     EYCLSTHLEGDGGNATGNCSGFAAATGNEFYRVFNLF"
     gene            14250..14651
                     /locus_tag="PKH60_02405"
     CDS             14250..14651
                     /locus_tag="PKH60_02405"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNQ16931.1"
                     /translation="MSKRLQKKSGFTMIELLAVVTIMAILFTIGIVAYTNAVRNSRDN
                     RRVTDISNMRQALVLFRSDTGCYPDDFADLLGSDGYWSEPQQPLDPSTGMSYTYTPSG
                     TCATGHSGFTITANMENTSTYPGGTYSVTNP"
     gene            14662..15219
                     /locus_tag="PKH60_02410"
     CDS             14662..15219
                     /locus_tag="PKH60_02410"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ16932.1"
                     /translation="MSRKNIYSQGFTLIELMATIVVSMILLITGGATYLQFQERQDAE
                     KVATELQRFAVKTRARARSQDTSGCTPGPLNKVIGYSLEFNEATNQVRSVGFCGTGKA
                     TASSDAVVATYAIPAPVDIEFGGSTVVDEGDVTATESVIFFSLMGGATVQNTNAGTID
                     VSRGGVTYRFIISPGGEISSVQKVP"
     gene            15221..15676
                     /locus_tag="PKH60_02415"
     CDS             15221..15676
                     /locus_tag="PKH60_02415"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNQ16933.1"
                     /translation="MVNQRGNTLIELLLATVIVGVVLTGVAISLTYSIQREALNRYRE
                     GATGLAQEVSEYLLLLRAQNNWQAFYAGVQDNADYCYSDNPVGITLGSGCPVITRLGM
                     DFTAAFSIDKPAVVPPAPEKVTANITISWEVDSDPKTYELTQEYYEREY"
     gene            complement(15673..16179)
                     /locus_tag="PKH60_02420"
     CDS             complement(15673..16179)
                     /locus_tag="PKH60_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16934.1"
                     /translation="MARNELGYSLVELLVVSSLLLLLLLGVSTLFIASLTADARITLR
                     QELRAEGNYALDSMSYFIRNAKAIPVCVSNGSTLSVLNEDGRVTEYYLSSNQIASHSA
                     ALAPFPERTDLLTVLPYEVETGSFVLDCNNVNQPYITISFTLTRTDSQGTAIENAFRH
                     TVLVRNQL"
     gene            complement(16161..>18285)
                     /locus_tag="PKH60_02425"
     CDS             complement(16161..>18285)
                     /locus_tag="PKH60_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16935.1"
                     /translation="NGGNPGSSAEFVCASTFGWNYGAPNDHPNTLCNNSCSYNVLVIG
                     VGDPTPTPTVTPGGPTNTPTPTVTPTPTITPTATATPTTPPASTPTPTRTPTPTPTVT
                     PTPTAVATPTSTPTATPTPTTPPDVAVVYGYVSLDTPPDTACNVLPQNGGQLADPGLC
                     YTRVIREAPGGGPPAGYDAGFGIDCFTEETAGNWYWWHNDPEADIISRHCQLDAGLLR
                     IINTNNPTYPNWTDCAADINQSNYPSLYNPNGDFVGGQITCTNGVANSILNGKNFRIY
                     PKYIAYTCGGGYRRSGYPRKRNFPQNLNSYSAGVSLVTPWAGDPFPGGFFIGRTDNGV
                     SERLKIDSSFDLAEVDESSAAPTSHSVTYTLNNTTGPYNFCYNEPTGPTPTPTPTPNP
                     SSTPTPTPTITPTPSPTPIPVITPWWQAQGGNIYSNNGFSVTLPDLAPAEAYSVDKNT
                     ITSANSSAGLPMCGDGGVINIHTYGRYSVNFSLFANVGKAENTHNNICQDFTFDYFAE
                     KLDVTSATIQALPAVNSFSSILTAADDQGDYYLYYHPGDFALNVSNIGDWNITDRTIV
                     VIGTPSTPANLVITSPGLLPSGGMQLADDAFLGFIVNGNIIIDPSIGNDIVANPTDEA
                     TNVAGLYFSTDGSIIVQNTTDPITRPEKRFVGYGTFIGCNGVTLERDFDNNTNKTEVF
                     RYNPNLPLTIPNILREAHVTWQEMN"
BASE COUNT         4899 a         3710 c         4189 g         5387 t
ORIGIN      
        1 tcgagccccc ttcggtccac aatttactta attaggaaaa tacactatgc gtgtaaaaga
       61 acttgtctca aaacttccag aactaggcaa agcgcttgag aggattttta aagatggtgc
      121 ggtttttgat tctagatatc cagttattag ctacacgggt gacttacaag ttgagaagga
      181 ccagcaatca gcagaaattg acgatacaaa gatcagtcag acctaatcct catcatccat
      241 ctgtttgaac gtcaaggcta cagcagccac gtagtcatca cccttgagtc gcattaagat
      301 tactccctgc gttggtcgag taaggattgg aatggatttc tttgtcatgg gtagcttgat
      361 ggtaatgccc tctctggtgg taattaccac gtctttgtga tcagcttcta ccaatctagc
      421 agaagcaaca ggaccggttt tggtagtgat atctgagact tttactccca tgccggtgcg
      481 tttttgactg ggatattggc tgagcacggt ccgtttgccg aggccccttt cggtaatcac
      541 taacaaagct ggctcgtctg aactaccggc caacttcgcc atttctggag taacagtttc
      601 aactcccact acgtaatcat ttgatttaag cgtgattccc ttaacaccct tggtgtctcg
      661 gttggaagtt ttgacttcat cttcgctaaa acgaatgact ttcccctgat gagaaatcat
      721 caagatgtca tcactaccgc cagtaagagt gctgcgaacc aatttgtcat catcattaag
      781 aataattgcg ataatgccag tttgtctgat gttttggtag agggcaactg cgctcttttt
      841 gactaagcct tttttagtag tcaaggtgat gaatttttct ttatctgctt cttcatcaat
      901 aatcagcaaa gattgaaccg tttcgtctgg ttgcaaactt agtagattga caatcgcagt
      961 acccttagcc tgacgattcg cttgaggaat atcgaaggct ctcattttga aaactttacc
     1021 ttggtttgta aaaagcaaca aggtatcatg agtttttgcg actagcaggg ttttaactat
     1081 atcttcatcg cgcattttta gacccacgct ccccttccct cctcttgatt gagaacggaa
     1141 agccccgggc tcaaggcgtt taatatagcc gctctcagtt agagttataa ctgtcggttc
     1201 attggggact aggtcctctt cgctgaattc cccaatcttg cctttaatta attgggtttt
     1261 acgttcatca ccatagagag ccttcaaatc gtgagtctct tgtttgacaa cggctaaaat
     1321 ttcaactgga ttctgtaaga gtaaaagtaa gcgatcgatt acttcttgga gctgtttgta
     1381 ctcgtcttct attttttgcc tttcgagggc cgccagacgc ttgagttgca tttccaaaat
     1441 ggcctgagcc tgaatttcac ttaggccaaa cttcgtcatc aatgcgattt tggcagcatc
     1501 ggtatcggct gactttctga tggtatcaat cacttcatct aagttgttga gtgcaatgag
     1561 caagccttcc aaaatgtgag cgcggtcgcg ggcttcggct aattcatatt ggcaacgttt
     1621 ggtgacaacc aattgtcggt gggcaataaa ctcagtaata atttgtttga tattcattaa
     1681 ttgaggaatg ccttcggagt tgagcgcgac catgttcatg gggaagctcg attgcaactc
     1741 agaatatttg aacagcttat tgaggacaac tttgggtcgc gcatcccgtt tgagatcgat
     1801 agtgatctgc ataccaactc gatctgagtc atcattaaga tccctaatac ccaaaatctt
     1861 tttgtctctt actaggtcgg caattttcat caacaatcga gctttgttaa cttgataggg
     1921 taattcggta atgataattt gaaagccctt gggaccttct tcaatgctgg ctttagctcg
     1981 aacgaccact ctcccttttc ccgtcttgta gccttcttta attgcagcaa aatcatacat
     2041 aataccacct gtgggaaaat ctggtccctt gatgtagttc atgatgtcat ctactgtggc
     2101 ttcagaacgg aatacaccag ctaaagtcgc tgcttccatg gccaatgttt tgtcaattag
     2161 gatatgcttt tcagcaatct cttttggaac tttagttatc attggttcag tggtagttcc
     2221 agattccagt tcggccaatt ctgctttgcc ctgctctatc acagatataa ttgcatcaca
     2281 gacctcattg agattatgcg gtggaatttt ggtggccata cccaccgcaa taccttccga
     2341 gcccatcagt agtaggttgg gcaatttggc tggtaagaca agtggttctt ggagtgagcc
     2401 atcaaagtta tctgtaaacg gcacggtttt tttatcaaga tcaacgagaa tttctgaact
     2461 aatttttgct agacgagcct cggtataccg catggctgca ggactatctc catcaaccga
     2521 accaaagtta ccttggccat caactaacat atagcgcatg ctaaagtctt gagccaggcg
     2581 aactaaagcc atgtacaccg atgaatcgcc gtgtggatgg tatttaccta aaacgtctcc
     2641 aacaatgcgc gcgctttttt tgtaggaact ggagtggtgg atgccggatt tgtacatgga
     2701 gtacaaaatc cggcgatgaa ctggtttgag cccatcacgg acatctggta gcgcgcgcgc
     2761 cacaattacg ctcatggcgt aatccaagta gctttgcttc atttcatcgg taatggcaat
     2821 gtatttcagt ttaccaaaac gactttcttg aatcgtgccg atgaactgct ccaagccatt
     2881 ggtttcactc acaacctcag cagtagtgtc tgttttattc tcacccactt cagcttgatt
     2941 atctagctcg aggttgttgt cttgatcgtt catttagatt tttctagtac tgctaatgcg
     3001 gttgtggcat ctgcccaatc tttgagcttg acccacttac cttcttctaa atctttgtaa
     3061 tgttcaaaaa agtgtttgat ttgacgcttt ttcatttctg gaatatcgtc taggctttcc
     3121 caggttccac agatgggatc aattttcatt ttggcgggaa cgcacactaa tttatgatcg
     3181 atccctgcct cgtcttccat ttccagcatg ccaatcactc ggcattttac tactgcgcca
     3241 ggaatgactg gtgcacttag gaatacgagg gcatcaagcg catcgccatc ctgacctttt
     3301 gtgccttcaa tcaaaccata attgacaggg taatccattg cggtgggcac aatgcggtcc
     3361 accatcattt caccagtttc ttcgtctagt tcatatttga ctcttgaacc ctctggaatt
     3421 tcgattttga ctaagacatc agacatagta ttctcctttt aaacgtcaag ctccgctaat
     3481 tgagcgtgcg tttggataaa ctttttacga ggagggacat catctcccat gagtgtgctg
     3541 aagattgaat cggctttatc cgcgtcatct atggttactc gcttgagcat tcgagtggca
     3601 ggattcatag tggtatccca cagttgctct gggttcattt ctcccaagcc tttgtagcgt
     3661 tggatgccga gcttgacatt ggcgttttct gattttaatt ttgcaactaa agtttcgcgt
     3721 tcttcttcag aatacgtata gtgcacatct ttgccggtgg ttattttgta taacggtggc
     3781 aaggcaatat acaaataacc ttgctgaact atttctctca gatgtcggaa aaagaaagtc
     3841 aatccaagtg tagtaatatg ttcaccgtct acatcagcat cattcataag gataattctg
     3901 tgatagcgca gtttttttac tgagaaggta tcgccaatac ccgaacccaa agctataact
     3961 aaatttttta attcttcaaa agcgacaatt ttatcgagtc gtgctcgctc agtattgagg
     4021 atcttacctc tgagcgggaa aatagcctgg aatcggcgat ctcttccctg cttggctgaa
     4081 ccgcccgcac tgtctccctc aacgatataa atttctgatt cggccgcatc tttgctttga
     4141 caatcagcga gtttgcccgg cagggtactt ccctcaagta atcctttacg gacgacagcg
     4201 tctttggctg ctcgagcagc cagtcgggct ctggtagaaa gcatgacttt acccacaatc
     4261 gctctggcgg cagctggatt ctcttcaaga aaggtggcaa aatcgtcttt aaatacttgg
     4321 tacactgctg gttgagcctc agaattattt aattttgctt tggtttgtga ttcaaactgc
     4381 aggtcatttg acggcatttt cacaaaaatc actgccgcca agccctcgcg cagatcttcg
     4441 gcactaaaga tttctttttc cttcattagt ttgttatcac tggcgtagtc tcggactact
     4501 ttcccaagcg ccatacgaaa gccattgacg tgagtaccac cgtctggagt gtgaatcaca
     4561 ttgacgtaag attcgagctt ttcattaaat gaatcgttgt attgcaaggc agtttctacc
     4621 acgatcttct tgcccgtttc aggatcagtc caggtttttt cactgtgaaa aaccggatgg
     4681 agtggtttac ggttcatgtt gatatgttca actaaagagc gaataccacc ctcaaaatag
     4741 tagtgttgag tttgtttttc tacctggtca tgtagttcaa aataaatccc tgccatcaaa
     4801 taggctcggt cggtaataat ttgtactaac gtttttgggc taaactgagt ggtagaaaaa
     4861 atagttggat ctggaataaa ttgcaataag gtaccagtct ccttctgaat caatcgagct
     4921 gattgctcag gaaagagttc ctggactttt ttttcatcaa tgaccgcaac tgatttttga
     4981 ggtacgcctc tcacatacgc ttggtaatga tactttttat ctcttttaat tacgacttgt
     5041 agtaatgaag acagagcgtt gacagctgac gaaccaattc catgcaagcc tccagatgca
     5101 ctataggcag tttcttcaaa tttccctccg gcatgtaatt ttgtcagtac tacttctagg
     5161 gcagatacgc cactctcatg catgtccact ggaatacccc gaccgttatc gataacagta
     5221 attgattcgc tgccaacctc gggaatacct gccaatttga tttcttctgc tgtagttggc
     5281 gagcggaaaa gttgaatagt accgccaaaa cctgcaattg cctcatcaac tgagttatca
     5341 agaatttctt tggctaaatg atgaagtccc ttggtgtcag tcgaaccgat atacatacca
     5401 ggtcgttttc tgactggttc aagaccttcc agaactttaa tctcacgagc gttatagtgg
     5461 ttgtcggtat ttgtcattgt ttgattggaa aacaagaatc actgttataa cacattctac
     5521 accaatttag taatggtctt ttgcaccaga aagcccaaaa aaggcacttt caagggctag
     5581 ttttacattg gcattttgct ctagtctgga gaaagccaac tgcaaacacc tcaattgcgt
     5641 cgatgaggga ataaaattgg tttgttgcgt taaccatcta agtaacgaag taatgagatt
     5701 gagagcgtca gtccgatcgg gatatttact tgccaacgaa atacactcag caattgaagc
     5761 ctggctaatt tcactaaaga gattggcaac ctcagcgcta ctctgctctt ttaaaacaag
     5821 gtgttcaagg gggactttta caatagagca tctggattga attgtcggta acactcggct
     5881 taaaatctgg gtgaccagcc aaaattgagt ttgtgttgtt tgggattcaa gtagtttgag
     5941 taaggcattt tgagcttcgg tagttgcatt ttcaaaccca agtaaaacaa acactctaat
     6001 ttgattatcg aggggggaaa aacttaattc agttgtaatt tgtctggctg tatcaatccc
     6061 aagtgattcg agatctggat ttatccactg gacggctgga tgaagcaagg catttgtata
     6121 cgggctgtat tgagcgagta tgccagcaat tgaattttgt tgatcagctt ccaccggcaa
     6181 aactaacaac aacggttgtt tataggcaga aaaaatagtt gccatggtgg tatcagtcat
     6241 tgttttggga ttgtatcgcg ccctaggttt ccagtatagt agaagggtgt tatgacagag
     6301 cctgccaatg cttaccgatc ctcaattact cctccgggat taatgcttcc tcaagttaat
     6361 gctgggcaag taccccaaaa tacaaccacc ttggtaggaa gtggtatggg tagtgtgatc
     6421 ccaccagcaa ttgcttcatc tagtgctgct aaacctaaat caattgatga gttaagcgtt
     6481 atccaaattc catcacaaga gccaacggta atttcttcaa acaacacccc aactcagcag
     6541 atgccaagta acaattctcc tcaactagca acgagtagtg gaattgattc tggcactgag
     6601 gatattccaa tccagtttat tggtgatgct ccaacggaag cagaaattga tacggctcag
     6661 gcttcaatta ccccttcaat tttgccgagt gaagtacccg taattcctat agatagtggc
     6721 ccacctactt ctgtagtcca aaacgcaact tcaccgtctg aaccgatcct agttgcccca
     6781 gaggtggtac ccaacccaat ttcagagccg gttgttacct ccagcaaacc agatgtacaa
     6841 gaaacccaat ctgtggttga accaccagtc aaagcctcag aaagtgtaga taatcaagca
     6901 aaaaaaacta aatccactgc cccactctca ctcttagaca tcttagttaa tcaacagcta
     6961 ctcactaccg aacaagcaaa cgaagttctc actcaaaata tccaaacggg actgaacatt
     7021 gagcatttga ttgaaaaaaa taatttagtt agtgaagaag acttggtcaa ggcaaggtcg
     7081 gaattttata acattccgta tgtagacctc aatgaagtcg gagtatatcc agaagcgctc
     7141 gcgcaagtgc cagaaacagt tgctcgcaat tattggatgc ttcctttcgc tttaaataaa
     7201 gataaaaaca cactttctgt ggcaatgaga aacccacttg acgtgcaggc agtcgatttt
     7261 gtggaacaca agaccggtca taaggtcatc gcacattttg cttctgcctc agaaattgaa
     7321 cggctcattg cggaacaata ttcgcaaagt ctctccacag aagtgactca agcattggaa
     7381 caaaccaagc aggtgcaaca gcaaaaagaa tctcaacaag actttggaga gctttctggc
     7441 ggcttgattc gtcaagcccc gattaccaaa atagtgcaaa ctattttatc tttttcgctc
     7501 aaggctcgag cttctgatat ccatattgaa cctcaagaaa atcgtactag ggtacgctac
     7561 agaattgatg gtattttgca agaaaagtta attttgccga aatctgtgca tcaggcggtt
     7621 gtttcaagaa ttaaaattct ctcaggttta aaaatagatg agcgtcggtt gccacaagat
     7681 ggtcggtttg attttagcga aaacaatcag caggttgatt tgcgtatctc tacactacca
     7741 accattcatg gagaaaaagt ggtgatgcgg ttgctcannn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnncgt atctctacac taccaaccat tcatggagaa aaagtggtga
     7921 tgcggttgct caataaaaat gccaacgtac ctactttgga ggaaattggg ctcagtggct
     7981 tagctctgcg gaatgttcag gatgccataa aagtgcctca tggaatcgta ttagtaactg
     8041 gaccaactgg atcgggtaaa acgactacgt tgtattcagt gttgcacacc attaacactc
     8101 ctagagtaaa tattatgacg ttggaagatc cagttgagta ccaaatgcca ggaattaacc
     8161 aagtgcagat aaatcctcaa gctgggctca cctttgcttc tggtttgagg tcattcctcc
     8221 gtcaagatcc caacattatt atggtaggag aaattcgtga tagtgagact gcgggattag
     8281 ctgttcaagc ttcgttaacc ggacacttag tttttgccac gcttcacacc tcctctgccg
     8341 ctggcgcact tcctcgtttg atggatatgg gtgctgagcc gtttttgctt gcttcttcaa
     8401 tgacttgtgt gttggctcaa cgtattgtgc gtattatcaa tccagactat aaagaagcgt
     8461 acaagccaga gccagcggtg attgaaaata tcaaaacggt gttaggcgat aattttgcac
     8521 gctggctcca acagaaccaa aaaactgagg cagaagttgt cctcatgcgc cccaaaaaag
     8581 atcgaccaga agccgagcca gattacaaag gtagagtggc tatttttgaa gttatgaaga
     8641 tctctgaaga aatagctaaa ttaattatgg cgcgcaaggc agccagtgaa atggaaaaag
     8701 ttagtctcaa agatggtatg ctgtttatga aacaagatgg gtatttaaaa gttcttgatg
     8761 gtgtgactac aatagaagaa gttttgagag tagccgaaat ttaatcgaat taaggagcct
     8821 ttatgagcac cactattacc attcatgacc tcatgcagaa agttgtagat ttgcagggat
     8881 cagatctcca cttagtctgt gggaatccag caattctcag agttgatggt gagttacgac
     8941 gggttgagat gccactgatt acgcctgcgc agaacaagga gctgattttt tcaatcttga
     9001 ctcaagaaca acaggattac gttaaggtca ataaagaact tgatttcggg tatcaatata
     9061 aggagaaggc tcggtttaga gtcaacgtct atcatgcctc aggagctttg gcgctagctg
     9121 cgcgtcttat tccagaaaaa attaaaacaa ttgaagaact cgatttatta cctattttgc
     9181 acgactttac actgtatcct caaggactgg tgttgttgac aggtccaact ggcgagggaa
     9241 aatcaactac gttggcggcg ttaattgatg aaattaacca taagcgcgca gagcatattt
     9301 tgacgattga agatcccatt gagtttattt atcaaccaga caagagtatt atttcccaaa
     9361 gagaagtcaa tcaagataca catggttggg atattgcgct ccgctccgct ctgcgcgagg
     9421 atcccgatgt ggtactgatt ggagaaatgc gtgaccacga aacaattgcc tcagcaatta
     9481 cgatagcaga aaccggtcac ttagtgtttg ctacgttgca taccaatacg gccagcgagt
     9541 cgatggacag aattattgat gtattccccg cagcacagca accacagatt cgccagcagt
     9601 tggcgggggt gattcgtgcg gttgtttcgc aacgattgtt gcccaaaaaa gggggtggca
     9661 gagtggctgc gcttgaagtc atgattgcca atgccgcagt caggaatcta attcgtgaaa
     9721 gtaaggcttt tcaaattgag tcaattattc aaaattcatc tgaacaaggc atgtttttat
     9781 tcgaaaaaca tttatttatg cttgttcagc aaggaaaaat tactgctgaa actgccagaa
     9841 accgagcctt tagaccagac gaaattgagc gcatgttagc aaaggagtaa tcagctatgc
     9901 catttttttc ttattctgca aaaaataaac tgggagaagt tgtcaaaggt aaggcagaag
     9961 cttcgagtat ggtggatgcc gcagcgttgc tggatgaacg aggattgctt gtcattaatc
    10021 ttaaaccagt tggtatggag ggtttgggca gtctactcca agggttcatg ggagttagtc
    10081 aaaatgatgt ggttaatttt actcgccagc tctctactat gatcagcgct ggtttatcaa
    10141 tttctaaggc tttaggtgta ctgattcagc agtcaactcc tggtttttca caactcgttg
    10201 ctaatttact ccatgaagtt gagggtggtg cgtctttttc aaaagccctc agttctcacc
    10261 caaaagtgtt ttcacgtctc tatgttcagt tggtcaaggc tggtgaggtg ggaggtattt
    10321 tggacgacat tttatctcgt ttggctcata atctagaaaa atcaaaagaa ttccgagcaa
    10381 aaacgaaggg cgccatgatt tatccagtca ttgtggtgct ggccatgcta gtggtcggtt
    10441 ttatcatgat gacgtttgtt ttgccaaagc tgacggctat gtatagtgac tttggcgtaa
    10501 cgttgccgtt gcccacaaga attttgatag gtgtatccaa tatttttgcc actcggtggt
    10561 atctcatatt ggctggtatc ggtggtggca tatttttgtt taatcgctgg tacaaaacag
    10621 aagcaggaca acgagtcata gcggggttac ttttgaaatt gccgattttt ggcaacatgc
    10681 tacagaaaat tatgattact gattttgtgc gcacactctc tttattgttg ggatccggcg
    10741 tgactttgct acaagctttg gatgtggtaa ttgaaggggt agaaaatgtg atttaccgtg
    10801 aagcctttac cgaggcgcgt aaccaagtag aaaaaggtgg tttgctgtca gaatctatag
    10861 ctcgtcacga aatgatcccg ctgattttgt cccagatggt ggcagttggc gaggaaacag
    10921 gaaaattgga tgaagtgcta cagaaactct cgacctactt tgagcaagaa actgagcagg
    10981 ctgtgaaaaa tatgaccaca gcgattgaac caattattat ggtaatttta gcagtggcag
    11041 tgggtggtat ggtgattgct attgtgatgc cgatatatag cttgacgagc gcgttttaat
    11101 tgttgacaag catcttctgg ctagtataga gtggatgata gtataaaagt tggtatttta
    11161 ttgttagtag ataataagga gaaatactat gaagaaagcc ttgcttgctc gcttaaaaag
    11221 cgggggtttt accatgattg agttgctcat cgtgatctcg attttgggta ttttagctgt
    11281 gatggttttg gcagcaatca atccactcga acaaattaat cgtggtcgtg atacagccaa
    11341 acgtggagat gccgaacagt tactcggagc gctagagaga tacaacgctt tcaatggttt
    11401 ttttccctgg caggaagacg caaatgatat aacgggctta gctctaacgg gtcagctggt
    11461 aaattctggg tgggtagaaa ctaattctaa ctgtcagatt ttagaacgat tgtcaactgc
    11521 tgttactgcg gggtgtgtgg gcactcaaga attaaaagat tcttacatca ctagagtaga
    11581 tgatgatggt ggtgggtcga cgggaactgt tgaagccagg ggtctctatg tctattatga
    11641 ggctgcggct ggagacaatg tttatgtttg tttttcacca cagtctaatg cctttgttaa
    11701 tgaggctact ctaagatgtg acggaacctt gccagacgat ctagctcctg caactggtat
    11761 ttgtgtagat gacgttactg ctaatttagc aggcgaaacc ggaattgata tgatctgtct
    11821 accataatct ggcagattgt tgagtttgaa aattaacccc gcctctcacg agaggcgggg
    11881 tttttttatc ccagtatttt ttttcttaac cttcgttatc aaacccctcc caattgtatt
    11941 ctccccgact ctgcggtaca gtaagcgggt gtttatattt caactaatgc ttagcttgct
    12001 gatttttgct cttggtgttt gtattggtag ttttttacat gttttggttt atcgcacggt
    12061 ggaagataaa gaagattgga aaactggtcg atctcgttgt gatcattgct cctacattat
    12121 taagtggtat gacaacatcc ccctactctc atttgtgtta cttgggggca agtgccgcaa
    12181 atgccacaag cctattgcca tctctcacgt agctatggaa gttatggttg gcgccctttt
    12241 tgtttggtgg tattgggcag gtagcgtttt tttcttgtta gtagaccagc catttactgt
    12301 gttgcaaccc ctcttttggt tgctcattgg cacgctcagt ctggttatct ttgtagcaga
    12361 tatgaagtac tttattattc ccgattgggc agtggcaatc atggcactag ccaccctggt
    12421 gtacagagtc gtattggtgc aaggaaaaat tatgcagcaa actgatttta tatcaatgat
    12481 attggcatct attggtgtcg gagccttttt ctggttactt agagtagtga ccaagggcaa
    12541 aggaatgggt tttggggatg tcaagctagc tcctgtattg gcattgttgg ttggctggcc
    12601 gcaagttttg gtggcactga tggtttcggt ttgttctggc gcattaattg gcagcttact
    12661 cttacttttg ggcaaacata agttaaaaca gcctataccc tttgggccgt ttctccttct
    12721 tggtagtgct gtagcgctgg tgtggggtga tgctatctgg ggttggtact ggactcaact
    12781 ccttgggatg tagtaggcta aatgcatgcg cttttttcgc tatagtttca tatttattgt
    12841 aatttttact tcgattgcaa ttggggcatt tttggcgatt caacagtggg tgttatttca
    12901 agcttacaat caactattgg cggatgcttc gttggtacag cggtcaaagc acgttggtga
    12961 gtactacgaa tattgcacgg aactaggggc ggaaaatgta gatgatggtc cactctatac
    13021 tccccaatta cgatttcttt ctgatactga atacgttttg gaagttgtat gtgatcagta
    13081 tgagtttgtc cccaagcagt ttggcgagaa aaaattacca ctgtttgcga gcaaaaagcc
    13141 aggtcaatcc gggctagtct ttgctgaaga aggtgtatgg tccatcagca tacaggctgt
    13201 gccagccgac ctagaaatct ggttaaaaaa aatagcacta ctcgaaaatt tagtatttcg
    13261 aactcaaaca atttcggttg aagacggtag tatggttaag tcactatctg taagcccaga
    13321 tgccattgag ccaattacca cttgtgaagg ttatggtttt agttgttgcg atccggtacg
    13381 cctgttgggc gtgggtgatc agcgcccagg tagcgattgt agtgatgatt gttactccag
    13441 ttgccatcaa cgaccaatca ttctctcttt taatgctgag ccgggatttg atagatacca
    13501 acgcatttta caagctagcc aggctgattt gattagtttt aattttgtga tcgattcctt
    13561 acttccagaa gaatctcaag tgacgcttga gtttggggat gggacaagtt ggattggcat
    13621 tgctacggaa actatgacaa accaccaata ttcatgcaat caaccagtgt gtaattatca
    13681 ggcaacttta aaagtagtgg atgcatatgg cacagtatcg gctagcactt cagccacaaa
    13741 aatgaatatt gctatacatc agtaagtaga cttgcaaagc atggctttac acaggtatga
    13801 tgaaagcact atgaaaaaaa atggttttac cctgatagaa attttgatag ttgtggcgat
    13861 cattggtatt ttagcaacta tgggaattgt tgcttttcag acgtcactta gaagaagtag
    13921 agatgcaact agaatagcgg aaatcaaagc tttgcagaat gcagctgagc aattctatgc
    13981 ggataatgct agtgaatatc ccacggttga tgggtgtgcg caattagcgc cctatcttca
    14041 aggttttcaa gattataatg acccacgagg cgttgcatac gaatgtgatt ttacaccggc
    14101 aggcgctgtt acatgggaat actgtttgag tactcaccta gaaggtgatg gtggtaatgc
    14161 caccggcaac tgtagtggat ttgctgccgc gacgggtaac gaattttatc gagtttttaa
    14221 cctgttttga ttaaggaggt gttatgctga tgtctaaacg actacaaaaa aaatctggct
    14281 ttactatgat tgagttactt gctgtagtaa ctatcatggc gattctgttt accattggta
    14341 tcgttgcata tactaatgct gttcggaact ctagggataa tcgacgagtg actgatataa
    14401 gtaatatgcg ccaggcgctg gtattatttc gatcggacac agggtgttac ccagacgatt
    14461 ttgccgactt attgggtagt gatggttatt ggtctgagcc acaacaaccg cttgatccgt
    14521 caaccggtat gtcatacaca tacacaccct cgggcacttg tgccactggt cactctggtt
    14581 tcacaattac agctaatatg gaaaacacat cgacctatcc tggtggtacg tactctgtaa
    14641 ctaatcccta aataaccagc aatgtctcga aaaaatatat attctcaagg cttcaccctg
    14701 attgagctca tggcaaccat agttgtcagc atgatcttgt taataactgg tggagcaaca
    14761 tatttacaat ttcaagaaag acaggacgcc gaaaaggtgg caaccgagtt acagcggttt
    14821 gctgtgaaaa cacgagcaag agcgagatct caagacacaa gcggatgtac tccaggtcca
    14881 cttaataaag ttatcgggta ctcattggaa tttaacgaag caacaaatca agtgcgatcg
    14941 gtaggatttt gtggtacagg aaaagctact gcatcctctg acgcagttgt tgcaacatac
    15001 gctattcctg ctccggttga tattgaattt ggtggttcga ctgttgttga tgagggagat
    15061 gttactgcaa ccgaatcagt catatttttc tctctcatgg gtggtgctac cgtgcaaaac
    15121 acaaacgctg gaacaattga tgtgagtcgc ggtggcgtga cctatcggtt tattatcagt
    15181 cctggtggag agattagcag tgtgcagaaa gtaccgtaat atggttaacc aaagaggtaa
    15241 tacgctcatt gaattattgc tcgcaaccgt gattgtgggt gtggttctga cgggtgttgc
    15301 tattagtctc acctactcca ttcagcgaga agcactcaat cgttatagag agggcgcaac
    15361 aggactggcc caagaagttt ctgagtattt actgcttctc cgtgctcaga ataattggca
    15421 agcgttttat gctggcgtac aggataatgc cgactactgt tattctgata atccggttgg
    15481 aataacattg ggtagcggat gtccggttat taccaggctt ggaatggatt ttactgccgc
    15541 ttttagtatt gataaaccgg ctgtagtccc tccagctcca gaaaaagtca ctgccaacat
    15601 aactatatct tgggaagtcg attccgatcc aaaaacctac gagttaaccc aagagtatta
    15661 cgaacgagaa tactaaagtt gatttctaac taagacagtg tggcgaaaag cgttttcaat
    15721 tgccgttccc tgcgaatccg tacgagtgag agtaaaactg atggtgatgt atggttgatt
    15781 cacattattg cagtcaagta caaaagaacc cgtctccact tcgtagggta aaactgtcaa
    15841 caggtcggtt cgctcaggaa aaggtgcgag agcggcagaa tgtgaagcaa tctggttaga
    15901 actaagataa tattctgtta ccctgccatc ttcattcagt actgatagtg ttgatccgtt
    15961 ggatacacat actggaatgg cttttgcgtt tcgaataaaa tagctcatcg aatcaagagc
    16021 gtaatttcct tctgctctta attcttgtct gagggtaata cgtgcatcag cagtcaaact
    16081 cgcaataaaa agtgtagaca ccccaagcaa taaaagtaaa agtagtgagc tcaccactaa
    16141 cagttcaacc agcgaatatc ctagttcatt tcttgccatg tgacatgcgc ctctctgaga
    16201 atatttggta tggtaagtgg cagatttgga ttatagcgga atacttcagt tttatttgta
    16261 ttattatcaa aatcacgttc aagcgtcact ccgttgcaac caataaaggt tccgtacccc
    16321 acaaatcgtt tttcaggtct ggtaattgga tcggtagtat tctggactat gatagatcca
    16381 tctgtggaga agtaaagccc ggcaacgttt gtagcttcat cagtcgggtt ggccacaata
    16441 tcattgccta tacttggatc aataataata tttccattga cgatgaagcc aagaaaagcg
    16501 tcatcagcaa gctgcatgcc tccgctcgga agcaaacccg gactggtaat aaccagattc
    16561 gctggtgttg aaggagtgcc aatgacgaca atagttctat cggtgatatt ccaatcgcca
    16621 atattgctca cgttaagcgc aaaatcacct gggtggtaat acaagtaata gtctccctga
    16681 tcatccgcag ctgtcaagat ggacgaaaaa ctgttaaccg caggcaaagc ttgaattgtt
    16741 gctgaagtaa cgtcaagttt ttctgcaaaa taatcaaatg taaagtcttg gcaaatgttg
    16801 ttatgagtgt tctcagcttt tccaacattt gcaaaaagtg aaaagtttac agaataacga
    16861 ccataggtat gaatgttgat taccccgcca tctccacaca ttggtaagcc tgccgagcta
    16921 tttgcgctgg ttatagtatt tttatcaacg gagtatgctt cagctggagc taggtcggga
    16981 agtgtaaccg aaaaaccatt atttgagtag atatttccac cttgagcctg ccaccatggt
    17041 gtgattacgg gtatcggagt tggagatgga gttggggtga ttgtaggtgt aggcgtggga
    17101 gtcgagctgg ggttgggtgt tggggttgga gtaggtgtag gtccagtagg ttcgttgtag
    17161 cagaagttgt atggtccagt tgtattattc aaggtgtagg taactgagtg gctggttgga
    17221 gcagctgatg attcatcaac ttcggctaag tcaaaggaac tgtctatttt tagtcgttct
    17281 gaaacaccat tgtcggttcg accaatgaaa aaccctccag gaaatggatc tcctgcccac
    17341 ggagttacta gtgatactcc tgcgctatac gaattaaggt tttgcgggaa atttcttttt
    17401 cgtggatagc cagagcgtcg ataccctccc ccacaagtgt aggcaatata tttggggtaa
    17461 attctaaagt ttttgccatt gagaattgaa tttgccacac cattagtaca ggtaatctga
    17521 ccgcccacaa agtctccgtt gggattatac agcgacggat aattagattg attgatgtct
    17581 gcggcgcaat ctgtccagtt aggatatgtt gggttatttg tatttattat tcttaatagt
    17641 cctgcgtcca actgacaatg cctggaaata atatcggctt ctggatcatt gtgccaccag
    17701 taccaattgc cagcagtttc ttcggtaaag cagtcaatgc caaagccggc atcataacca
    17761 gcgggcggtc cacctcctgg ggcttctcta attactctgg tatagcaaag acctggatcg
    17821 gctaactgac cgccgttttg aggaagtacg ttacacgcag tgtctggtgg agtatctagc
    17881 gaaacatagc cgtaaaccac tgcaacatcc ggaggtgtgg tgggggttgg agtagcggtg
    17941 ggcgtggatg ttggtgtagc cactgcagta ggtgtaggag tcacagttgg agtgggtgtt
    18001 ggtgtgcggg ttggggttgg tgtagaagct ggtggagttg ttggagttgc tgttgcagtg
    18061 ggggttatgg tgggcgtggg ggtaacagta ggtgtgggcg tattggtggg accaccagga
    18121 gtgaccgtgg gagtgggcgt tggatcgcca accccaataa cgagtacatt ataactacag
    18181 ctattgttgc agagagtatt ggggtgatca tttggggcac cataattcca accaaaagtg
    18241 ctagcacata caaattcagc tgacgaccca ggattacccc cattt
//
LOCUS       DAOQZP010000022        16423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_3954_length_16423_cov_5.827652, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000022 DAOQZP010000000
VERSION     DAOQZP010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 16423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16423
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3954_length_16423_cov_5.827652"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PKH60_02430"
     CDS             <1..350
                     /locus_tag="PKH60_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16936.1"
                     /translation="RRLYEKIGGLNESIFMYAEDMEFCLRANRAGWGSAIAHLAKIVH
                     VGSASSNSQQAKLGEVRGMLQFWPKYFGETSTLLLKLIFSFGSCLRFCLFSLIGRGAQ
                     ANIYSKILRQLWV"
     gene            388..2112
                     /locus_tag="PKH60_02435"
     CDS             388..2112
                     /locus_tag="PKH60_02435"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNQ16937.1"
                     /translation="MLRILKKLARWFDHNGVLFFAGLLVLLIPLYPKIPIFSPIEQYI
                     VRVRLEDFVVAIAVAFTFIQILRKKARFDKIVLFLMSAYALAGILSMLSAIFISQTVP
                     MQPLHIAKTALHFFRYMEYFSVFFVSFAAIRSKKDVYTLLKLLVVAVFAVALYGYMQK
                     FFYWPVYSTMNREFSKGIRLVLTEHARIQSTFAGHYDMAAYLVIVLPIILSLALTIRN
                     KKKKIIIYLVFFSGFWLMVMSASRIPFAAILFGLLLTILLHAIIKTKLVDKFTFLFSR
                     TLVSSIFLLFMLYYFGQNMLDRISNAISSSDNPININKMIDDVLDPLPLPQASQLLAW
                     LPKAPALPENSEAAPDLEAAIIAQVASIGDQPPVPKNPTPTAVVADRPRDVYEDIPEP
                     VDTYETLADGTIQRVVIHKTRVYSDCALKHELSLCIRLESLWPWALQSFTTNPIFGTG
                     YATLNKQFVDEFTIADSTDNNYLRVLGETGLLGFLTFYGLCMYLLISTGLSIKKLKSG
                     MDRALAIGFTGGAIGLLINATYIDVFAASKVAYSFWFFAGLIRANYLLPRVSTIKKTK
                     QKRRHVDH"
     gene            2099..3547
                     /locus_tag="PKH60_02440"
     CDS             2099..3547
                     /locus_tag="PKH60_02440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNQ16938.1"
                     /translation="MSITKWIQSLPLVFILLLAISLRLFRVNQTVLDWHSFRQADTAS
                     VTREYVKNGIDVLHPKYHDLSNIQSGQDNLDGYRMVEFPIIQASLALIIRTLPWIDLV
                     TLSRLASLLASLGTIAAIYYLGLLWSGKKVAAFAALTFAILPYSVYYSRAVLPEPFLL
                     ASSTGAIAYWHYWLAKGKWHYYWYAFILFALALLLKPFAAFVLPVFIAIAAVQSIWRK
                     PKHLIAALFFSATAVPLFLWRWWILQFPSGVPAADWLLNGNGIRLRPAWFRWLGYERL
                     TKLILGYIGPVFLILSLFRTKFSERILYTSWWLGMLAYVIVVASGNVQHDYYQNLLLP
                     IISLTVARGMVQLAWILKNTFKINKPWLSVNVLYAVMIVLATFQVKGFYQMNHSEYVK
                     AGRVVDEQTPEDALVIAPAFGDTQFLFQTNRRGWPIGFEIDKKIKLGAKYYISTSYDD
                     EARELESKYFTIIKTQDYILLDLTKSASPSKP"
     gene            3556..4758
                     /locus_tag="PKH60_02445"
     CDS             3556..4758
                     /locus_tag="PKH60_02445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNQ16939.1"
                     /translation="MLTPYLPFPLLSGGQIRTYNLLKQLANKHEITLFCLIKDDTERQ
                     YIPEIKKYCHEIRVFRRSQHPFTLKNIFHTAFSRYPFLVIRNHVPSVIKATKLELAQH
                     HYDLIHAETFYMMPHLPETSTPVILVEQTIESLGYESYAQKAWWPLKKILDIDIQKIR
                     HWEQHYWDIADRLIVMSEEDKLAITPHVNQPKKIAVVANGVDIAWFGQVKKKTHKNPT
                     ILFVGTFKWLPNREAVSYLVKKVWPLIRKNLPQAELHIVGNSPSDTVLGYAKAEHGIR
                     ITGNIPDIRDAFATADVLIAPVFSGKGTRYKVLESLASGTPIVATSTAVEGLGLKPGI
                     HVEIGDSASTLAQKTIDLLQDPVRRKSMATEGLSFLTKRYDWPLISEKLDTIYQQMGS
                     ARHGKKNF"
     gene            4739..5617
                     /locus_tag="PKH60_02450"
     CDS             4739..5617
                     /locus_tag="PKH60_02450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ16940.1"
                     /translation="MAKKTFDLAIIIVTYNSQFWLEKTLQTLDSFYLQKTKRSVETVV
                     VDNNSSDATQSMMLKQFPWVTFIQLETNTGFAAANNRGISAVAAKNYMLMNSDIELTD
                     NSNLDTLINFASKRKDVGAISPRLLFSDGKMDMACHRGEPTIWASLTYFLGLEKLFPQ
                     STTFGQYHQTYKDLTTIHTIEACSGATMLVKQSTIKKVGPLDERFFMYAEDLDWCKRF
                     RDAGLLIVYHPGVTLIHHKYKSGLKNASQKIAKQTKRHFFDTMLQYYDKHYAHHYPSV
                     VRWLVKIFIVIRKGAV"
     gene            5614..6069
                     /locus_tag="PKH60_02455"
     CDS             5614..6069
                     /locus_tag="PKH60_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16941.1"
                     /translation="MNLHKFQIQYLRQKNLQALMEALVVMIGAIFVAILLPQVLVRFI
                     YANQQLTAEPMLLQYIPVVALVLGVLQLVVTLLGNFLRERQMVKLERTLVNEVNQSTG
                     AIDESELKELEKLVDSALTKHSPKKGVKKSSPTNKSTTKQAARTKKVSK"
     gene            6076..7173
                     /locus_tag="PKH60_02460"
     CDS             6076..7173
                     /locus_tag="PKH60_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNQ16942.1"
                     /translation="MRIGIDCRLAGPDHAGIGRYTAELVNNLLSTSNTIEWVLFCSNQ
                     QQAEYLIGSNKSKRVRAVICTIQHYSLAEQFLLPWIFYREKLDLLHVPHFNVPLAYRK
                     PYFITIHDLLWHHHRGQQVTTLHPWIYWLKYLGYRLVASQAIKTAKHIFVPSEEIKQT
                     LHQYYPTMRMKIEVTYEGVSLEPPQILRKSLPKNYLLFVGSLYPHKNIAVVLKALQEL
                     PELQLVLVGSRSVFSQQIHQLVTTLDVEKQVTFFGRATEGELHTLYKNAYALVQPSLS
                     EGFGLTGIEAMAAGTPVIASDISIFHEVYQDAAVFCQPHDPKSFVQAIQQLPKHSKDL
                     RALGLKLSKQYSWQKMASATLSAYQRFLAHS"
     gene            7170..8411
                     /locus_tag="PKH60_02465"
     CDS             7170..8411
                     /locus_tag="PKH60_02465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ16943.1"
                     /translation="MSQNAVAKPSVAIVYDRATVAYGGAEDLLQVLHQAFPSAPLYTS
                     VWNPQLGWTKNWPVVTSFLQNIPWGSKLHRLLAPLMPIAFESLNLTDFDIVISVSSAE
                     AKGVITKPNQLHLCYLFTPTRYLYEYKTAYGDSAGWHNLPIISFAISLVRKYLTWWDV
                     QVAQRPDKYLTLSHKIQKKIQQMYKRDSEVLYPPVVINPNDVAPEIVKFARSNHYALS
                     LSRLVTYKRVKDSIDACHKSKLPLLIVGSGPDFDRLTQLYPSQTHIRAKDQSLAACLD
                     LAFSNHKSIVFLGSVTNEERTTLLLYAKILFMLGDEDFGISAVQAALLHVPTIVSEQS
                     GASELLQSIPNSIQLQKLTPSEICLAIQTAISFENKSPDLSQLSKQTSPEFFISQIKE
                     IVYDEWRKHMKKYDNVRRRIQ"
     gene            8408..9517
                     /locus_tag="PKH60_02470"
     CDS             8408..9517
                     /locus_tag="PKH60_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNQ16944.1"
                     /translation="MNQNSHVYAVILAGGGGTRLWPKSRNDTPKQFLKLMGKHTMLQI
                     TAQRLNSFIPWEKMVVVTNQKYSKRVGELLPELPKNNIILEPEKKDTALAMLVGSIFA
                     HNLDPKAVVVNAAADHVVQDESEFIRVMKAAADVAAENEFLVTVGISPKFASTAFGYI
                     KISDSIRQSSRAASLFKVESFTEKPNETTARAFLATGKYFWNANMYVWATQTIITAFQ
                     QLQPEIWKSAKALVGKSIDDFTKSLESIYASVEPISIDYAISEKADNLVLVPGDFGWD
                     DIGEWLVVYNLGQKDLAGNVIISDGDQDRLPTLSVESQNNLIHSNGRLVALLGVRDMI
                     IVDTPDIVMIAPKSRSQDVKKLVERLKEEKRSEYL"
     gene            9514..10164
                     /locus_tag="PKH60_02475"
     CDS             9514..10164
                     /locus_tag="PKH60_02475"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNQ16945.1"
                     /translation="MNWPKHFQQAFCYEFTKRILDILVATLLTILFLPFWIIVPILIY
                     LDSGSPILFRHRRLGKDGAEFYLYKFRSMVHNADDILHNQDKKLLEEFKNGDWKIKND
                     PRITKIGKVLRSLTIDEFPQLINVLLGDMSMVGPRAYLQKELDEQSKKYPETKGYIPL
                     ILSVKPGITGVWQTSGRNEIPFIQRAKIDAAYASRHSIIEDIVLLFKTPKAMISKW"
     gene            10193..12133
                     /locus_tag="PKH60_02480"
     CDS             10193..12133
                     /locus_tag="PKH60_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HNQ16946.1"
                     /translation="MRIALPRLPKKSATPTPNGKGVVKKQLPKKRILLAITIPLLLLI
                     GILGGVSIYSFTVVQQIKQQAFVLQYTGQAVAQQLSAQNLPGAKAELEKVPSELESIK
                     TEFNKLSFFNYLPITKEYYADGLAGFTAAEAGLAAAQKAIYAVEPYADVLGLNGQTPT
                     ASGSGTAENRIKLILDTLDKAMPELDAITANIQSAVTALQQIDANRYPEQLGGQPVRP
                     LILQAQNLSQSALSAVTEFRPVIEQIPVMAGGRGERLKYLVLFQNDNELRPTGGFLTA
                     FSTIYIEDGKVTPETSDDIYALDAKYRSRLPIPEALGRYLTTEKYWNLRDMNTSPDFK
                     VSMEQFLPNYMKVGDKNIDGIITVDTEVLKQLLEVLGPVQVPGYGTFSAEIDKRCDCP
                     QVVYALSEIITRPTNYIREDRKGILGPFMRAVLSKAYDAPSEKNGPLFQAMIEAIQGR
                     HIQVYYIDESLQKAAEAINVAGRMTPPTKGEDFFAVINANLGGAKSNLFTTYEMVQQI
                     EAPTEGKIKKTVEITYKNNRRADNCNLEAGLLCLNSTLRDWTRIYVPAGSELIEATGF
                     TKDAKIYDELGFTVFDGFFILEPNGVAKIRLTYSVPYALPDYQLQLWKQGGITDYPLT
                     MEVTGGRDELTINKDTLYTTTW"
     gene            12146..12691
                     /gene="recO"
                     /locus_tag="PKH60_02485"
     CDS             12146..12691
                     /gene="recO"
                     /locus_tag="PKH60_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNQ16947.1"
                     /translation="MLQRNFTAVGLVLKRVKVGETDVIITMLTQERGKLVCRAKGVRT
                     LKSTKRSALQPGNIVRFHWLETKSLPLLTQALMEEDCSVMESTLIRMRQLSQLLELCE
                     RLFIESELEEKIFNQIVKLRQSIVQNRLGSTDVRTTFRSIITQLGYQDPEDSKYESIG
                     DYISALSDKPMRSYNFLSVKG"
     gene            12935..14341
                     /locus_tag="PKH60_02490"
     CDS             12935..14341
                     /locus_tag="PKH60_02490"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNQ16948.1"
                     /translation="MSDQNVSLEAVVSLCKRRGFVYPTSEIYGGLTSSYDFGPLGAQL
                     LKNIRSAWWHTFVESRPDMVGLDTQILLHPQTWVASGHVDSFNDPLVEDVKTHKRYRA
                     DHLLEGWLKLQNEAPAELTDKSVEDLTVTQMGEVIAKYQVLSPEGNPVSEPKQFNLLF
                     ETAIGSVADEKAKVYLRGETAQGIFSNFKQVLNSTRVQLPFGIGQIGKSFRNEVTTGQ
                     FIFRTLELEQAEIEYFFDPDATNWQELLEQWKQQMWHFVTETLGISAEHLQWRQHTDT
                     ERSHYSKDTYDLDYQFPFGFKELWGVAYRTNYDLTQHQNVSGEDLSYRDPQTGKTFLP
                     HVIEPALGIGRTMLMILCEAYWEDTERQRTVLRLKPSLAPYQVAVFPLLKNKPELVAQ
                     AQEIFKTLLPTMRTTWDDRGNIGKRYLYQDEIGTPACVTIDFQTLEDQTVTVRDRDTT
                     DQKRLHLDELSAYLNSMQ"
     gene            14363..15160
                     /locus_tag="PKH60_02495"
     CDS             14363..15160
                     /locus_tag="PKH60_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16949.1"
                     /translation="MNKKILIPSIVGLLVLVIVGIVVFSRTGKSDQTTETTPTKKMAP
                     EQVNTIPVAERPFIQIHPESDGRNVTLTVVSLKKTASETEYELEYQAGSLLQGAFGKL
                     SLASLPSSVKILLGSCSAGGACTYHTDIQGGTLLTRYADDSKYALKSEWRYFDNTTKS
                     PEVASKDAKFQLTSPELAKQRYSIVFNTPGYPDGLEGTVVSEIFSLETSSTLKGDGDL
                     TLRANQEGVTHVAVWDGAEWSYIVATVEGKTITAEVPLAPLYLAVTK"
     gene            complement(15184..15354)
                     /locus_tag="PKH60_02500"
     CDS             complement(15184..15354)
                     /locus_tag="PKH60_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16950.1"
                     /translation="MNPEKLNEKGKRKSIQELWDDSDRITQDILAHPWKHPLQYVLMV
                     LGRMPDKEKKVD"
     gene            15460..15882
                     /gene="mraZ"
                     /locus_tag="PKH60_02505"
     CDS             15460..15882
                     /gene="mraZ"
                     /locus_tag="PKH60_02505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNQ16951.1"
                     /translation="MFIGRYYHHLEDKGRISLPKTFREQVTDWIVTRGLDGGLFVFPK
                     AEYQQQLQQIAASTFTKQSHRDFVRLLTNEAREVQADSQGRVQLPEYLIEFAHLVKQV
                     VIVGSYSWIEIWDQDRYHQYLEQVEPRAEAIAESVSPL"
     gene            15879..>16423
                     /gene="mraW"
                     /locus_tag="PKH60_02510"
     CDS             15879..>16423
                     /gene="mraW"
                     /locus_tag="PKH60_02510"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HNQ16952.1"
                     /translation="MKQSEHHQPVMLAEAIEMLSIKPGHWYIDATFGRGGHTSKMIAL
                     GGLVLALDFDQEAIQFAQEHFSAEISDCKLIIKRANFTEIESIWRAEASLKDLRPQGI
                     LFDFGTTSDQLLSPTRGFSFESDAPLDMRMDDRLGVTAADFLQVLSEKELANMFRNFG
                     GESYAQPIARKIKQASPVTTTR"
BASE COUNT         4871 a         3626 c         3307 g         4619 t
ORIGIN      
        1 cccgcagact ctacgaaaaa attggtggtt taaacgaatc gatttttatg tatgccgaag
       61 atatggaatt ttgtctgaga gctaaccgtg cgggttgggg aagtgccatt gctcatctgg
      121 ccaaaattgt tcacgtcggc tctgccagca gtaattcaca gcaagccaaa cttggcgaag
      181 tgcgaggaat gcttcaattt tggcccaaat actttggtga aacatctacc ttgctgctca
      241 aactaatttt ttcctttggc tcttgcctac gtttctgcct atttagtctg ataggacgcg
      301 gtgcccaagc caacatctat agtaagatat tacgacagct ctgggtttga cctgctccag
      361 tagcaccgcg tacaatacgc tcacactatg ttgagaattc tcaaaaagtt agcccgatgg
      421 tttgatcaca acggcgtgct gttttttgcc ggcctacttg tcttacttat ccccctgtat
      481 ccaaaaattc ccatttttag ccccattgaa cagtatatcg ttcgtgtccg actggaagat
      541 tttgtcgttg ctattgcggt tgcattcact ttcatacaaa ttctcagaaa aaaagctcga
      601 tttgacaaga ttgtcttatt tctaatgtct gcctatgcac tcgcaggaat tctctccatg
      661 ctgagcgcaa tattcatcag tcaaaccgtg ccaatgcaac ccctccacat tgccaaaact
      721 gccttgcact ttttccgata tatggaatat ttttctgtat tttttgtaag ttttgctgca
      781 atacggtcaa aaaaggacgt ctacacacta ctcaaattac ttgtcgtcgc tgtctttgcc
      841 gtagccctgt acggctacat gcaaaagttc ttttattggc cggtttactc caccatgaat
      901 cgggaattca gcaagggaat ccgcctagtc ctcaccgagc atgccagaat tcaatccact
      961 tttgcaggac actacgacat ggccgcatac ttagtcattg ttctccccat catcctttct
     1021 ctcgcgctta ctatccgaaa taaaaagaaa aagataatca tctatttagt atttttctct
     1081 ggtttctggc tgatggtgat gagcgcatct cgtataccat ttgcggcaat tctgtttggt
     1141 ttgctactaa caatcctgct tcatgcaatt attaaaacca aacttgttga taagtttacc
     1201 ttcctctttt cgagaacatt ggtttcgagt atttttctac tttttatgtt gtattacttt
     1261 gggcaaaaca tgctcgaccg gattagcaat gccatcagct catcggacaa ccccatcaat
     1321 attaataaga tgattgatga cgtgttggat ccattaccat taccgcaagc ctctcaatta
     1381 ttggcttggt tgcctaaagc tccagcttta ccagaaaact cagaagcggc accagattta
     1441 gaagccgcaa ttatcgccca agttgcttca attggagatc aaccaccagt accaaaaaat
     1501 ccaactccca cagcggtagt tgccgataga ccaagagatg tttacgaaga tatccccgaa
     1561 ccagttgaca cctacgaaac cctcgctgat ggcaccatac aacgagtcgt cattcataaa
     1621 acccgcgtct attcggattg cgccttgaaa cacgaattat ccttatgcat tagactagaa
     1681 tcactctggc cgtgggcact gcaaagcttt actacgaatc caatttttgg caccggttat
     1741 gctaccctca acaaacaatt tgttgatgaa tttaccattg cagatagcac ggataacaac
     1801 tatttacgcg ttcttggcga aacaggctta cttggtttcc tgacttttta tggtctctgc
     1861 atgtatctac tcatttccac tggtttgtcc atcaaaaaac tgaagagtgg gatggatcgc
     1921 gcactcgcca ttggcttcac tggcggagcg atcggtttac taatcaatgc tacatatatt
     1981 gacgtctttg ccgcttcaaa agtagcatat agtttctggt tttttgctgg acttatcaga
     2041 gcaaattatt tactaccgcg cgtatccacc atcaaaaaaa ctaaacagaa acggcgtcat
     2101 gtcgatcact aaatggatcc aaagcttgcc acttgttttt attttactgc ttgccatatc
     2161 gcttcgctta tttcgtgtta accaaacagt gcttgattgg cacagttttc gtcaagccga
     2221 taccgcctca gtcactcgtg aatacgtcaa aaatggaatt gatgttttac atcccaaata
     2281 tcatgatttg agcaacatcc agagcggcca agacaatcta gacgggtatc ggatggtcga
     2341 attcccaatc attcaggcgt ctctcgcact cattattcga acgcttcctt ggatagatct
     2401 tgtgacgctc agccgccttg cctctctatt ggcgtcactc ggtacaatcg cggcaatcta
     2461 ttatcttggc ctcctttggt ctggtaagaa agttgctgcg tttgccgcac taacttttgc
     2521 aatcctacca tattcggtct attactccag agcagttctg ccagagcctt ttttactagc
     2581 tagtagcact ggtgcgattg cgtattggca ctattggctt gccaaaggca aatggcacta
     2641 ttactggtat gcctttattt tgtttgcttt ggcattatta ctcaagccat ttgccgcttt
     2701 tgtcttaccc gtgtttatcg caattgcggc agttcagtcc atttggcgca aaccaaaaca
     2761 cctaattgcg gcgttgttct tttctgcaac ggcagtcccc ctatttctct ggcgatggtg
     2821 gatactccaa ttcccttctg gggttcccgc agccgactgg ctactcaacg gcaatggcat
     2881 tcgattacgc ccagcttggt ttcgctggct tggctatgaa cgcctaacaa aacttatact
     2941 tggatatatc ggaccagtat ttttgattct gagtttgttt agaacgaaat tttctgagcg
     3001 cattttgtat accagttggt ggctgggcat gctggcatat gtaattgttg tcgcttcggg
     3061 caacgtccag cacgactatt atcaaaatct cttgttgcca attattagcc tgacagtggc
     3121 ccgaggcatg gtgcaactcg cctggatttt aaaaaataca tttaaaatta acaaaccatg
     3181 gttgagcgtc aacgtgctct atgcggttat gatcgtactc gctactttcc aagttaaggg
     3241 gttttaccaa atgaaccact ccgaatatgt taaagcgggc agggtggtgg atgaacaaac
     3301 accagaagac gcactagtga ttgcccctgc atttggtgac actcaatttt tatttcaaac
     3361 taatcgaaga ggttggccaa ttggatttga aattgataaa aaaatcaagc ttggcgcaaa
     3421 gtattacatc tccactagct acgacgatga agcgcgagaa ctagaaagta agtattttac
     3481 aattatcaaa actcaagatt atattttgct tgacctaact aaatcagcca gtccatccaa
     3541 accatgagag tcttaatgct taccccctat ctaccgtttc cactgctctc gggtggtcaa
     3601 attagaacct acaatttatt aaaacaactc gcaaataagc acgaaattac tctcttctgt
     3661 ttaatcaaag atgatacaga aagacaatac attccggaaa ttaaaaaata ctgtcatgaa
     3721 atcagggtat ttcgccggtc tcaacatccg tttaccctca aaaatatttt tcataccgcg
     3781 ttttctcgct atcccttttt agtgattcgc aatcacgtac cgtcggtaat taaagccaca
     3841 aaattagaac ttgcccaaca tcattacgat ctcattcatg ccgaaacatt ttatatgatg
     3901 ccgcatctgc ccgagacatc tacgccggtg attttggttg aacaaaccat tgaatctttg
     3961 ggttacgaaa gttacgctca aaaagcctgg tggccactca aaaagattct ggatatcgac
     4021 attcagaaaa ttcggcattg ggaacagcat tattgggata tcgcagaccg tctaatcgtt
     4081 atgagcgaag aagacaaact cgctattact ccacatgtta atcaaccaaa aaaaattgcg
     4141 gtggtagcca atggggtaga catcgcatgg tttggtcaag taaaaaagaa aactcacaaa
     4201 aatcccacta ttctgtttgt gggcaccttt aagtggttgc ccaatcgtga ggcagtgtcg
     4261 tatttggtaa aaaaggtttg gccactcatt cgtaagaact taccgcaagc agaactacat
     4321 attgtgggca attctccctc agatacagtg ctcggttatg ccaaagcaga gcatggtatt
     4381 agaatcaccg gtaatattcc cgatattcga gatgcctttg ccactgctga tgtcttaatt
     4441 gcccctgttt ttagtggcaa aggcacccga tacaaagtgc ttgaatcttt ggcatcggga
     4501 acccccatag tggcaacatc tactgccgtt gagggattag gtctcaagcc aggtatccat
     4561 gtagagattg gtgattcagc ttcaacatta gctcaaaaaa caattgattt gttgcaagat
     4621 ccggttcgta gaaaatcgat ggccactgaa ggtctgtcgt ttttaaccaa acgctacgac
     4681 tggccactaa tttcggaaaa gcttgataca atctaccaac aaatgggaag cgcacgacat
     4741 ggcaaaaaaa acttttgatt tagcaattat tatcgtcaca tataacagtc aattttggct
     4801 agaaaaaacc ttacagacct tagattcatt ttacttgcaa aaaaccaaac ggtctgtaga
     4861 aacggttgta gtggacaaca acagcagtga tgcaacgcag agcatgatgc tgaaacaatt
     4921 tccctgggtt acttttattc aactggaaac caacactgga tttgccgccg ccaataaccg
     4981 aggaatttct gccgttgcgg ccaagaacta catgcttatg aactcagata ttgagttaac
     5041 agataactct aatctagaca ctttgataaa ctttgcttcc aagcgcaaag atgtaggggc
     5101 aatttctccc agacttctgt tttctgacgg aaagatggat atggcctgtc acagaggaga
     5161 acctacgatt tgggcgagcc tcacctattt tcttggctta gaaaaattgt ttcctcaatc
     5221 cacaactttt ggtcaatatc atcagacata taaggatcta accactattc atacgattga
     5281 agcctgttct ggagctacta tgttggttaa gcagtcaaca attaaaaaag ttggtccact
     5341 tgatgagcga ttttttatgt acgccgaaga cttggattgg tgcaaacgtt ttagggacgc
     5401 tggcttatta attgtgtatc accccggcgt tacgcttatt caccataaat acaaatctgg
     5461 attaaagaat gcttcacaaa aaattgctaa gcaaacaaaa cgacactttt tcgataccat
     5521 gctacaatac tatgacaaac actatgctca ccactatccg agcgtagtac gttggttagt
     5581 aaaaatattt atagtgatca gaaagggagc agtatgaacc tccacaaatt tcaaatacag
     5641 tatttaagac aaaaaaattt acaagcacta atggaagctt tggtggtaat gattggtgca
     5701 atttttgttg ctattctgct tccccaggtt ttggttcgat ttatttatgc caatcaacaa
     5761 ttaacagccg aacccatgct cttgcaatac attccagtag ttgccttagt acttggagtc
     5821 ttacaattgg tggtcacact gctcggtaat tttttacgag aacgtcaaat ggtcaagctg
     5881 gaaagaactt tagtgaatga agtaaatcaa tctacaggtg ccattgatga gtcagagctc
     5941 aaagaacttg aaaaactggt agatagtgcc ctcaccaaac actcaccaaa aaaaggtgtt
     6001 aagaaatcca gtcctaccaa taaatcaacc acaaagcaag ctgctcgtac aaaaaaagtg
     6061 agtaaataat ctctcatgag aatcgggatt gactgccgct tagcaggacc cgatcacgcg
     6121 ggtattggtc gctatactgc agagttagtc aataacttat tgagtacctc aaacactatt
     6181 gagtgggtct tattttgctc aaatcaacaa caggctgagt acctaatcgg tagcaataaa
     6241 tccaaacgag tccgcgcggt aatttgcacc attcaacatt attctcttgc ggaacaattt
     6301 ttgctaccct ggatctttta tcgggaaaaa cttgacctac tgcacgtgcc acactttaat
     6361 gttcccctag cgtatcgcaa gccctatttt attacgatac atgatttgct gtggcatcat
     6421 catcgtggtc aacaagtaac aacactgcat ccgtggatat attggttaaa gtaccttggt
     6481 tatcgcttgg tggcttctca agctattaaa acagcaaaac acatctttgt accttcagaa
     6541 gaaattaaac aaactttaca ccaatactac ccaactatgc gaatgaaaat tgaggtgaca
     6601 tatgaaggag taagcctcga accacctcaa atattgcgca aatcattgcc taaaaattac
     6661 ttactatttg taggctcttt gtatccacat aaaaatattg cagttgtttt gaaagccttg
     6721 caggaattac cagagttaca gttagttcta gttggttcaa gatcggtgtt ttctcagcaa
     6781 attcaccagc tagttactac tctcgatgtg gaaaaacaag taactttttt tggtcgcgca
     6841 acagaaggag agttgcacac actgtacaaa aatgcgtatg ccctagtcca accctcactt
     6901 tcagaaggtt tcggcctcac tggtatcgaa gccatggcgg ctggcactcc tgtaattgcc
     6961 tcagatattt cgatttttca tgaggtttac caagacgccg cagttttttg ccagccgcac
     7021 gatccaaaaa gttttgtgca ggcaatccaa caattgccaa aacatagtaa agatctaaga
     7081 gctctaggtt taaagctcag taagcagtat tcctggcaaa agatggcaag cgcaacttta
     7141 tcagcctacc aaagatttct tgcccactca tgagccaaaa cgcagttgct aaaccatctg
     7201 tcgccatcgt ctatgaccga gccactgtgg cgtatggtgg ggcagaagat ctgcttcaag
     7261 ttttgcatca agcgtttcca tcagccccac tatatacctc agtttggaat cctcagttag
     7321 gctggaccaa gaactggccg gttgtgacta gttttttgca aaatatcccc tggggatcca
     7381 agttgcatcg actattagct ccgttgatgc ccattgcgtt tgaatcgctc aatttaactg
     7441 atttcgatat tgtcatcagt gtcagtagcg cagaagctaa gggagtaata actaaaccga
     7501 atcagctcca tctgtgctac ctatttactc ccactcgcta cttatacgaa tacaagactg
     7561 cgtatggcga ctctgctggt tggcataatt tgccaatcat tagcttcgct atttcgctgg
     7621 taagaaaata ccttacttgg tgggacgtcc aagtagcaca aagaccagat aaatacttga
     7681 cgttatctca caaaattcaa aaaaagatcc aacaaatgta taagcgtgac tctgaagtgt
     7741 tatatcctcc agttgtcatc aatccgaatg atgtcgctcc agaaatcgtc aaattcgctc
     7801 gatctaacca ctacgctctc tccctatcca gattagtcac gtataaacga gtcaaagatt
     7861 ccatcgatgc ttgtcataaa tctaaacttc ccctgctcat tgtgggtagt ggtccagact
     7921 ttgaccggtt aacccagctg tatccaagtc aaacgcatat tcgggctaag gatcaatctt
     7981 tagctgcatg tcttgatctg gctttttcaa accacaaatc gatcgttttt ctgggctcag
     8041 tgaccaatga ggaacgcaca acgttactac tatatgccaa aatcctcttt atgctgggag
     8101 atgaagattt tggcatctct gccgttcaag cagctttgct acatgtaccc actatcgttt
     8161 cggagcagtc cggcgccagt gagttattgc aatcgattcc aaattctatt caactacaaa
     8221 agctcactcc gagtgaaata tgtctggcaa ttcaaactgc catcagtttt gaaaataaat
     8281 ctccagatct ttcacaactt tcaaaacaaa caagtccaga attctttatt agccaaatca
     8341 aagaaatcgt gtatgatgag tggcgaaaac atatgaaaaa gtatgacaat gttcgaagga
     8401 ggattcaatg aatcaaaact ctcacgtcta tgcggtgata ctagctggtg gtggtggcac
     8461 tcgtttatgg ccaaaatctc gtaatgatac ccccaaacaa tttttgaaac tcatgggaaa
     8521 gcacaccatg ctccaaataa ctgctcaacg attaaactcg tttattcctt gggagaaaat
     8581 ggttgttgtt accaatcaaa aatactcaaa aagggttggt gagttgctac cagaattacc
     8641 aaaaaataat attattttgg aaccagaaaa aaaagatact gctttagcaa tgcttgttgg
     8701 gtcaattttt gctcacaatc tcgacccaaa ggcagtcgtg gtcaacgccg cagcagatca
     8761 cgtcgttcaa gatgagtctg aatttattcg cgtgatgaaa gcagctgcag atgtagctgc
     8821 agagaacgaa tttttagtca cagtcggtat ttcgcccaaa tttgcctcta ctgcttttgg
     8881 gtatatcaaa attagtgatt ccattcggca atcatcaaga gccgcttcgt tatttaaagt
     8941 ggaaagtttt accgaaaaac ccaatgagac aaccgcgaga gcatttctag cgactggcaa
     9001 atatttttgg aatgccaata tgtatgtgtg ggctacccaa acaatcatca cggcttttca
     9061 acaattgcaa ccagaaatat ggaagtcggc caaagcgctc gttgggaaat caatagacga
     9121 ttttaccaag tcgttagaat caatttatgc ctcagtcgag ccaatttcaa ttgattatgc
     9181 aatctcagaa aaagcagaca atctggtatt agtaccaggt gactttggtt gggatgatat
     9241 tggagagtgg ttagtagtgt ataacttagg ccaaaaagat ttggcgggca acgtcattat
     9301 cagtgacggt gatcaggata gattgccaac actttctgtt gagtcccaaa ataacttaat
     9361 ccattcgaac ggccgattgg tggcattact tggtgtccgt gatatgatta ttgttgatac
     9421 tccagatatc gttatgattg ccccaaaatc tcgcagtcag gatgtaaaaa agttagttga
     9481 aagacttaaa gaagaaaaac gttctgaata tctatgaatt ggccaaaaca ctttcagcaa
     9541 gcattttgct atgaatttac aaaaagaatt ctcgatattc ttgtagcgac tctcttaaca
     9601 attctgtttt tacccttctg gatcattgtg ccaattttga tctacctcga ctctggttct
     9661 ccaatattgt ttcgccatcg tcgattgggc aaggatggcg ctgagtttta tctgtataaa
     9721 tttcgttcaa tggtacacaa tgcggatgat attcttcaca accaagataa aaaactgctc
     9781 gaagaattta aaaatggcga ctggaaaata aaaaatgatc ccagaattac taagatagga
     9841 aaagtattgc gttccctcac aatagatgaa tttcctcaac taattaacgt tctgcttgga
     9901 gatatgagta tggttggtcc tcgagcctac ttacaaaaag aacttgacga acagagcaaa
     9961 aaatatcctg aaactaaagg atatattcct ctgattttat ctgtaaaacc aggaataact
    10021 ggtgtctggc aaaccagcgg tcggaatgaa ataccattca tccaaagagc aaaaattgat
    10081 gccgcatatg ccagtcgaca tagcataata gaagacattg ttctcctgtt taaaaccccc
    10141 aaagccatga tatctaagtg gtaattgcta acatctgata tactgtttat ctatgagaat
    10201 tgccctgcca cgtcttccca aaaaatcagc cacgccaacg cctaatggga aaggagtggt
    10261 aaaaaaacaa ctgccaaaaa agagaatctt actcgccatc accataccac ttttgcttct
    10321 tattggcatt ttgggaggtg tttctatcta ttcattcacg gttgttcagc agataaaaca
    10381 acaagcattt gtgcttcaat atactggtca agcagtagcg caacaacttt cggctcaaaa
    10441 cctgcctgga gcgaaagcag aattagagaa agtcccctca gaattagaat caatcaaaac
    10501 agaatttaat aaattaagtt tcttcaatta tcttccgatt accaaggaat actatgcaga
    10561 tggtttagct gggtttactg cggctgaagc aggtttagct gctgctcaga aagcgattta
    10621 tgctgtagaa ccctacgccg atgtgttagg actcaatggc caaaccccaa cagcatcggg
    10681 tagcggtaca gccgaaaatc ggattaaact tattctcgat actctggata aagccatgcc
    10741 agaattagac gcaatcactg caaatataca gtctgccgtc acagcattac agcaaattga
    10801 tgctaataga tatcctgaac aacttggggg acagccagtc cgtccactta ttttgcaagc
    10861 acaaaatttg tctcaatctg cactcagcgc agtaactgaa tttcgaccag taatcgagca
    10921 aattccagtt atggctggcg gaagaggcga gaggctgaag tacttagtgc tctttcaaaa
    10981 tgataatgag ttacgaccaa ccggaggctt tttaactgct ttctcgacca tttatattga
    11041 agatgggaaa gtcacaccag aaacttctga tgatatttat gcgttggatg caaaatatcg
    11101 ttccagatta cctataccag aagcactggg aagatactta accaccgaaa aatattggaa
    11161 tttgagagac atgaatacct cccctgattt taaagtttca atggagcagt ttctacctaa
    11221 ctacatgaaa gtaggtgata aaaacatcga cggtattatc actgttgata ctgaggtttt
    11281 gaagcagtta cttgaggtgc taggtcctgt ccaggttccc gggtatggca cgttctcagc
    11341 cgagattgat aagcgttgtg attgccccca agttgtgtat gcactatcag aaattattac
    11401 tcgaccaacg aattatattc gcgaagatcg aaagggtata cttggcccct tcatgagagc
    11461 ggtgctttca aaagcctacg atgcaccaag tgagaaaaat ggaccattat tccaagcgat
    11521 gattgaggcg atacaaggaa gacatattca agtctattat attgacgaat cattacaaaa
    11581 agctgctgag gcaataaatg ttgctggtcg tatgacacct ccaactaaag gagaagactt
    11641 ttttgctgtt attaatgcta acttgggtgg cgcaaaatca aatctcttta ccacttacga
    11701 gatggtgcaa caaattgaag cacccactga aggaaagatt aaaaagacgg tagaaattac
    11761 ctacaaaaat aatcgtcgag cagacaactg taatttagaa gctggtctgc tttgcttgaa
    11821 ctcaacactt cgagactgga caaggattta tgtgccagct ggctcagaac tgattgaagc
    11881 tactggtttt actaaagacg caaaaatcta tgacgagcta ggcttcactg tgtttgatgg
    11941 attttttatc ctagagccaa atggcgtagc aaaaattaga ctaacatatt cagtaccata
    12001 cgctctccca gattatcaat tacagctctg gaagcagggt ggtattaccg attacccact
    12061 taccatggaa gtaactggtg gcagagatga gctcacgatc aacaaggata ccttatatac
    12121 aacgacatgg tagactgttg tgagtatgtt gcaacgaaat tttacagcag ttggactggt
    12181 tctcaaacgc gttaaagtgg gggagactga cgtgataatc acgatgttga cccaagagcg
    12241 cggcaaactc gtttgccgcg ccaaaggagt ccgcaccctc aaatccacta agcgttcagc
    12301 gcttcagcca ggtaatattg tacgatttca ctggcttgaa acaaaaagtt tacccctact
    12361 cacccaagcc ctgatggaag aggactgctc agtgatggaa tcaaccctaa ttcgcatgcg
    12421 ccaactttcc caacttttag aactttgcga gcgcttgttt atagaatcag aacttgaaga
    12481 gaagattttt aaccaaatcg taaaacttcg ccaaagtatc gtccaaaatc gcctcggatc
    12541 aactgatgta cgcacaactt ttcgctccat catcacccaa cttggctacc aagatccaga
    12601 ggattctaag tatgaatcca ttggtgacta cattagcgct ctttctgata aacccatgcg
    12661 gagttataat ttcttgagcg tgaaaggata gtcctatagt attggtattg acatcgatgg
    12721 tttaatcgtc tacaatgatt ccatatttac tttgctatct gcccttggca agcaaataaa
    12781 agatacaagg taaagtacaa aaagacgtct tggatcgagg ctttagagct tgtttcagga
    12841 aagaggggtg gaaccgccca acccgggtcc cttcatcagt agatatcgct gatgggggtc
    12901 tttttgtacc tttttcagct taagaggagt gtgtatgtct gaccaaaatg tatcgcttga
    12961 agcagttgta tcgctgtgca aacgcagggg gtttgtctat cccacatcag aaatttatgg
    13021 tggactgact agttcctatg attttggacc tttgggagca caactgctca aaaatattcg
    13081 atcagcatgg tggcatacat ttgtagaatc cagaccagat atggttgggc tcgatactca
    13141 gattctgtta catccccaga catgggttgc ctctggccac gtcgactctt tcaacgatcc
    13201 cttggtggaa gacgtaaaga cacacaaacg ctacagagcc gatcatttgt tggagggatg
    13261 gctgaagttg caaaatgaag ctcccgcaga acttacagat aagtcggttg aagatttgac
    13321 tgttactcag atgggtgaag tgatcgccaa ataccaagta ctcagccccg agggcaatcc
    13381 tgtgagtgag ccaaaacaat ttaatttgct gtttgaaacc gccatcggtt cagtcgctga
    13441 cgaaaaagcc aaagtgtatt tgagagggga aacagcacaa ggtatcttta gtaacttcaa
    13501 acaagtactc aattctactc gggtacagtt gccctttggt attggtcaaa tcggtaaaag
    13561 cttccgcaat gaagtcacca ctggtcaatt tatcttccgc acactagagc ttgagcaagc
    13621 tgagattgaa tatttctttg atccagacgc caccaactgg caagaactgc tggaacagtg
    13681 gaaacagcaa atgtggcact ttgtaactga aacactgggt atttcggcag aacatctgca
    13741 gtggcgtcag cataccgata ccgaacgaag ccactattca aaagacacct atgatttaga
    13801 ttatcaattt ccttttggat ttaaagagtt atggggggtc gcgtaccgga ccaactacga
    13861 cctaactcag catcaaaatg tttcgggcga agatctcagc tatcgcgacc cacagacagg
    13921 aaaaacgttt cttcctcacg ttatcgaacc ggcgctgggc attggcagaa ctatgctcat
    13981 gatactctgc gaagcttact gggaagatac cgagcgacaa cgaactgttt tacgactcaa
    14041 accatcgctt gcaccctatc aagtagccgt gtttccccta ctaaaaaata aacctgaatt
    14101 agttgcccaa gctcaagaaa ttttcaaaac actactacca acgatgcgca ccacctggga
    14161 tgaccgaggg aacattggaa aacggtacct ctatcaagat gaaattggaa cgccagcatg
    14221 cgtaaccatt gattttcaaa ctttggaaga tcaaactgtt acagttcgag atcgagatac
    14281 aactgaccaa aaacgacttc atcttgatga gttgagtgcc tatttgaatt caatgcaata
    14341 atctcgtata ctcactcttt gtatgaataa aaaaatcctt atcccaagta ttgttggtct
    14401 tcttgtacta gtgatcgtgg gtatagttgt attttcgaga acgggcaaat cagaccagac
    14461 tacagaaact actccaacta aaaaaatggc tccagagcag gtaaatacca ttcccgtggc
    14521 agaaagacca tttattcaaa ttcatccaga atctgatggc agaaatgtaa cgttaacagt
    14581 agtttctctt aagaaaaccg catcagaaac cgagtatgag cttgagtatc aagccggctc
    14641 actactacaa ggcgcctttg gtaaactctc tttagcatct ttgcccagta gtgtcaaaat
    14701 attgcttgga agctgtagtg ccggtggtgc ctgtacgtac cacactgata ttcaaggcgg
    14761 aacgttgtta acaagatatg ctgatgactc aaaatatgcg ctcaaatctg aatggcgcta
    14821 ctttgataac acaacaaaaa gcccagaagt tgcatcaaaa gatgccaaat ttcaacttac
    14881 ttcacccgag ttagctaaac aacgctactc aattgtgttt aatactcctg gatatcctga
    14941 tgggttagaa ggtacggtag tttctgaaat tttttcactt gaaacctcct caaccctcaa
    15001 aggtgatggc gatctaactc tccgcgctaa ccaagaaggt gttactcatg ttgctgtttg
    15061 ggatggagca gagtggagct atatcgtagc cactgttgag ggcaaaacca ttacagctga
    15121 ggtaccactt gcaccacttt atttagcagt aaccaaatag tgtctaaaac tactcactaa
    15181 tctttagtct actttttttt ctttatcagg cattcttcct aacaccatca aaacatactg
    15241 aagtggatgt ttccatggat gagcgagtat gtcctgagta atccggtcac tatcatccca
    15301 caattcctga attgattttc tctttccttt ttcgttgagt ttttctggat tcatagttgt
    15361 cattctacaa catttcgtct tttctttgtc tttacagttt gtgctagtta tgtgatagtt
    15421 gttgctggtg gtaaaaagtg gtaaagtatc aaaatagaaa tgtttattgg cagatactac
    15481 caccaccttg aagataaagg aagaatctca ctgccaaaaa catttcgtga gcaggtaaca
    15541 gattggatcg ttacccgcgg ccttgatgga ggactgttcg tattcccaaa agcggagtac
    15601 cagcagcaac tccaacaaat cgccgcaagc accttcacaa aacagtcgca tcgagatttt
    15661 gtccgcttac ttactaacga agcgcgtgaa gtgcaagctg actcccaagg tcgagtacaa
    15721 ctgcccgagt acttgattga gttcgctcac ttggtaaaac aagtagtgat cgtagggagc
    15781 tactcttgga tcgaaatttg ggatcaagat aggtatcacc agtatcttga acaagtcgaa
    15841 ccaagagccg aagccattgc tgaatcggtc tcaccgctat gaaacaatca gaacatcatc
    15901 aaccagtcat gttggctgag gccatcgaaa tgctctccat caaaccaggc cattggtata
    15961 ttgatgccac atttggcagg ggaggccata ctagtaaaat gatagctctg ggaggtctgg
    16021 tcctagccct cgattttgac caggaagcca tccagtttgc tcaagaacat ttttcagctg
    16081 aaatcagtga ttgcaaactt attatcaaaa gagccaactt cactgaaata gaatctatct
    16141 ggcgagcaga agcgagcctc aaagacttaa gaccgcaggg aatcttgttc gattttggta
    16201 ccaccagcga tcaattactt tcacccactc gtggctttag ctttgagagt gacgcccccc
    16261 tggatatgag aatggacgat cgcttgggag tcaccgcagc tgatttcctc caagtgctga
    16321 gcgaaaaaga gcttgctaac atgtttagaa actttggtgg tgagtcatac gctcaaccta
    16381 ttgcacgaaa gattaaacag gcttcaccag tgacaactac ccg
//
LOCUS       DAOQZP010000023        16205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_4014_length_16205_cov_5.244211, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000023 DAOQZP010000000
VERSION     DAOQZP010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 16205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16205
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4014_length_16205_cov_5.244211"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="PKH60_02515"
     CDS             complement(<1..915)
                     /locus_tag="PKH60_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNQ16953.1"
                     /translation="MPRIRASQLVAIVTIVVLLLFQQQAEIGKVRASLGIKTIVPTQL
                     VAVPAVPVASQRWQAIIASNHENDLWITGSDVTEYPKISAKSVYVEDLASESILLSVN
                     ESTQLPPASTQKLMTALVALESFTPETVLTVQSSDIQWSNILDFTVGEQFIVEDLLKS
                     LLIVSSNEAAEVLAREYPGGYQAFITRMNEKASEIGLENTSFRNPTGYDAVGQYSSAK
                     DLHLLSNHAMSHPLIKAIVKEPLLIISDVTGNRKHVLKSTNQLLEKNLGVVGIKTGTT
                     ELAGEVLISQFHLDEHQLRVILMGSSQRY"
     gene            complement(926..2341)
                     /locus_tag="PKH60_02520"
     CDS             complement(926..2341)
                     /locus_tag="PKH60_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNQ16954.1"
                     /translation="MKIQTLPIFDCTKAVGQTVTIVGWVDSKRDHGKVTFIDLRDSTG
                     KVQCVGYQKMGEVTTESVLKIVGEVKQRPEKMVNSDLLTGSIEIDVHEYEVLNLAKEL
                     PLPIDDDGYAISEEIRLQYRYLDLRRQRMQKNLRLRSDYGQAWRTILHENKFVEVETP
                     MLTKSTKEGSRDFIVPSRFQPGKFYALPQSPQQYKQLLMTAGVERYFQFARCIRDEDL
                     RADRGFEHTQIDLEVSFMDQNGVMNLIENMVKQAVTQVGGKLRDQQFPIFSYKDAIAQ
                     FGKDKFDLRSESEKTDGTLAFAWVVDFPFFKEVDSTDPAEVADGKSGWTFTHNPFSMP
                     KPEYLERHLKGESISEIVTTQYDLVCNGYEVGGGSIRAHRPEIVKATFATMGYSDAEI
                     ESSIGHMLRAFEVGTPPHGGIGLGFDRQCMLLAGEQSLKEVVAFPMSSTGRTAVMDAP
                     SSIEDTLLKELGLQVVTNNKK"
     gene            2408..2842
                     /locus_tag="PKH60_02525"
     CDS             2408..2842
                     /locus_tag="PKH60_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16955.1"
                     /translation="MSSRTREIPSVDNLLGLQYYHKIGQLAPTDFTPPMFEWLKLIKE
                     LPQLSDVELHEFMKFMIFFQTQLNQKYEEGSFLHKIYQIDPQTIVREIGVRSATIQNV
                     ILYMVNKLKQTTELEFTADYQIGINRFLSEQIALLKHVLQLQ"
     gene            complement(2817..4490)
                     /gene="recJ"
                     /locus_tag="PKH60_02530"
     CDS             complement(2817..4490)
                     /gene="recJ"
                     /locus_tag="PKH60_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNQ16956.1"
                     /translation="MTSQKAQWHLMHEQSPNDVVELQKWLLEDRGIKNVEKFLLPPKP
                     NALTLNDVGIVASQMKKAVARLIQAKKMDQKVVIAGDYDADGICATAVLWLACKEMGL
                     IAQPFIPNRHTHGYGLNQRSIPDMIGENKPDLVVTVDNGIVAHEALKTLSKMGIDVII
                     SDHHEKASTEPNALAIVHTTQLCGAGVAWFLAKHLLKTTETPGADTNTNQLLDLVSIA
                     TIADQVPLIEANRSLVFHGLRQLKTTQRHGLQALFEMGNIDQSKIDEVGVSFQIAPRL
                     NALGRLSHGMDALRLLCGGTKEFAKQRARLLEEANIERQEITQDLYARANQLAQEQTA
                     AKILIIAGSEFHEGIIGLLAGRLVEQYGKPAIVISVNETLAKGSVRSIPGIHITDFLR
                     QMSNSFLELGGHAMAAGFALEPATVDKVSEKIQVLANKLTIKNAAKQIDITCPLKSEL
                     IDLDTILTIESLSPFGQANPRPLFLLSDWQVVGVKTIGNEGKHLKLVIANMNQLDNQK
                     TVIEALWWKHGDQSIKFQTGTKISLVGRLEKQTWRNKSFPQFIVIEVRV"
     gene            4522..6159
                     /locus_tag="PKH60_02535"
     CDS             4522..6159
                     /locus_tag="PKH60_02535"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HNQ16957.1"
                     /translation="MLQVPSQLTLALEPLIVLHELNKAGFANYIVGGAVRDLLLSNEK
                     WLFIDFDLTTNASPQQLLTIFPFAFYENTFGTVSLTYQHLWDFAGIDLEYRKARELVV
                     TSHQIRKTNPTLIDLLNATKIHTSLLEHTEKPPLEPQKAVLPLIEVTTFRSGEKYQNG
                     ARRPSEVQWGHSLIEDLKRRDFTINALALKLDPDWIETTFKQFTTHPPNLQGIPLDQS
                     VFELIDRHNGITDLESGLVRTVGIATERIQEDALRMLRAIRFAVQLGFTLDPELVEAI
                     KQHALLLKDVSPERIRDEFFKMLVSDHPKQAIELLDETQLLEQVLPELLSAKGVRQGG
                     HHTTDVWQHSIDALQACPSKDPIVRLATLIHDIAKPATYDDAKGQPTFYNHEVIGARV
                     AKQIGQRLRLSKNDCDRLFVLVRYHMFHYQPENSDAAIRRFMRKVGLQHIDDILDLRE
                     ADRLGSGARKTSWRLEEMKERMVAQLHQPFDSNDLAIDGHDLIDQLGLKPGKLIGTIL
                     QTLLEIVLENPEKNTKEILLEIARAELEQSKQKKSVA"
     gene            complement(6162..7049)
                     /gene="secF"
                     /locus_tag="PKH60_02540"
     CDS             complement(6162..7049)
                     /gene="secF"
                     /locus_tag="PKH60_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNQ16958.1"
                     /translation="MINLFKRPWLFFFISGTILIPGLISLAVNGLRPSIDFTGGTVLE
                     VSYSGEQEPTVEKLSAIWGENYPVVSVQKTGENQVVIKSAFVIDEQKQNAITQLGDQF
                     GDLEQLRFETIGPVLSRELLIKTLTALALVSIIITLYVWYRFHELRYGVSAIFAMFHD
                     SLILLGVFSLLGKYLGIEVDTLFVTGILTTLSFSVHDTIVVFDRIRELRPKFRRLPFR
                     TVANTAVIETLSRSINNSVTIIIMLLALYLLGGNSIKNFALALLIGAITGTYSSPFIA
                     VPFLFFWDSMASWIQKRKR"
     gene            complement(7042..8376)
                     /gene="secD"
                     /locus_tag="PKH60_02545"
     CDS             complement(7042..8376)
                     /gene="secD"
                     /locus_tag="PKH60_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNQ16959.1"
                     /translation="MTFSISLMIAAPSLRYEKINVAGKDFSIPLGIPKVNFSLLGKQI
                     STDLPFKKGLDIQGGMQVVLEADMSQVDPADRETALESAKAVIMRRVDLYGISEPLVQ
                     TARVGDSFRIIVELPGVEDPGEALSLVGQTAQLQFMLVSSMTPPEGMPASIAAQLQPE
                     VEDVGLSGIDLQRAAVVLNEQTREPEISLQFNGQGAEIFAKVTTDHTGETLGIFLDGV
                     PLMLPTISTPILTGQAVITGQFTLDEAKQLSIQLNAGALPVPISVLEQRTIGASLGQE
                     AVSRSIIAGLIGLGLVLVFMVLLYGWQGVIADMALVVYAVLTLAIYKVAGITLSLPGI
                     AGLLLSIGMAVDANILIFERMKEELRRGKPHAIAMELGFGRAWDSIKDANAATILTAL
                     VLINPLSFSFLNSSGLVRGFGLTLLIGVVLGLFTGVVVTRTFVRLFLKAKND"
     gene            complement(8412..9017)
                     /locus_tag="PKH60_02550"
     CDS             complement(8412..9017)
                     /locus_tag="PKH60_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNQ16960.1"
                     /translation="MTSLQDLMISRVRVKIIELFFSQTNEMFFVREITREVKEEINAV
                     RRELDRMIGFGLLKSEQRGNRLYYFLNPRYAFFQELRQMVAKCTGLGKEIRRLRRKLG
                     TVQFVMFSSRFAGGLKEKRGELDMLVVGDIVLSELEALVKQEQDRIQREINYTVFSSD
                     EFEFRKTRRDPFIMDVLYGGRVMIIGNEVDFVDRGIPGISQ"
     gene            complement(9073..10278)
                     /locus_tag="PKH60_02555"
     CDS             complement(9073..10278)
                     /locus_tag="PKH60_02555"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HNQ16961.1"
                     /translation="MKSKQFLLILLVLYIGSAIGTYAVRAMFDTSPASINSDGQSVPT
                     FEEETALGALLSIDPSEPKDQSCPLNGKLYTKTEAAAWQARRPLAVMIENSPDARPQS
                     GLSSADVVFEAVAEGGVTRFMAMYYCAVQAVDTTIAPVRSARTYFVDWASGFNRPLYT
                     HVGGANTPGPANAIGQIADYGWALENDLNQFSIGYPTFVRNAARLDREVATEHTMETT
                     SEKLWAVAEKRGWTNMSPEMKVGRTVLPAKDWQEGYTGWTFASSNEAPGTITSIAYDF
                     WSGYNQYSVKWQFDATTKTYARTLAGEPHVDLNTNKQISASVVIVLLTAEKGPIDELK
                     HMLYKTTGSGKALVYQNGQEITATWTKKTRESELQFTDSKGKELVFQPGLVWISVVDD
                     STTVNTSGV"
     gene            complement(10312..10563)
                     /locus_tag="PKH60_02560"
     CDS             complement(10312..10563)
                     /locus_tag="PKH60_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16962.1"
                     /translation="MAKIRTKKAKISAKQHRASAPAVTQKQGVDPMDRANLDTHEIDT
                     TTLLGYPIKLLYRDLFRSIAITLILVVLLLGIFVYFRYN"
     gene            complement(10565..11731)
                     /locus_tag="PKH60_02565"
     CDS             complement(10565..11731)
                     /locus_tag="PKH60_02565"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNQ16963.1"
                     /translation="MAILIVLLVLSFLVIIHELGHYFAARKAGITVEEFGIGYPPRAL
                     NFSLLAKRFNITKYFSFWRRLDSSRTELTLNWIPFGGFVRMAGETPDPKQNATPQPGD
                     FLAATLAQRLMVIFAGVAVNFIFGVAAFTIVFSKTGIPQEISGARIGTVEPNSPAAQA
                     GVPNQVQIIGFLDDDGLVETKTVESVKSFVEENRGETVTLITTGICQQLICEDRQEYS
                     VYLRTADETPENQGSLGISFETVIFQSYPWYEMPFRSAWHGSVQALQLSKMILDALSG
                     VGQDLFRGKVSSDVAGPVGIVYQAQKGQFFDQGWLSILTFAGILSINLAIMNILPFPP
                     LDGGRAVLSILELLIGKNKSHLIEYYANYGGYVFLLALVVLITFSDISRIIMGG"
     gene            complement(11750..12304)
                     /gene="frr"
                     /locus_tag="PKH60_02570"
     CDS             complement(11750..12304)
                     /gene="frr"
                     /locus_tag="PKH60_02570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNQ16964.1"
                     /translation="MTDAINHFQQKISKSYEHVLQDVRSLRTGRASVQLLDPVRVEAY
                     GTQMSLVEVASVTAPDATLLVVSPWDKSLITSIEKAIASAGLNLNPVVDGQIIRIAVP
                     ALTQERRQEMVKLLHQKIESGRVTVRTIRTETKQEIEEQKGGAGVSEDDIKLELDELD
                     KEVKLALTKLDELQAQKEKELLHV"
     gene            complement(12324..12719)
                     /locus_tag="PKH60_02575"
     CDS             complement(12324..12719)
                     /locus_tag="PKH60_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16965.1"
                     /translation="MKLFSKEPQIQQTEFTASQTWLEEPSLTPAEQLERAEKKTRQRK
                     VIMISVGSIALIIAIILLMVNLLSRPEPEIVEVVAPTPTPTETPILTPLLSRILEAKQ
                     RTEAIDVREAELALPQVEFEIVLDEPKKQ"
     gene            complement(12716..13570)
                     /locus_tag="PKH60_02580"
     CDS             complement(12716..13570)
                     /locus_tag="PKH60_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16966.1"
                     /translation="MKRLVVLFSLIFLVGLLRAAHVTAQTEELTPTVTTIEESVEETT
                     VEKDIEDERQALKALYRDQIQSYQNSYRKFSLAKTQFQNLATLASLEEAVLSTREVFS
                     ARNIVLITYLDQLFLELTVNPSYDEEIRQEYLNKIKQLRAELVTFNQLVINSQDRQAL
                     AERAVGFIPLGTELQSISVQTRKLLFLGKFRSSAGMAQQLTQEIKASHEANPVSALKQ
                     GERQRAYQELTSADAVVQTAWEKSVLQYQTEEDFSNQAYTAFIVTLSPLQSGLIRWYT
                     LLEQAAQL"
     gene            complement(13567..14286)
                     /locus_tag="PKH60_02585"
     CDS             complement(13567..14286)
                     /locus_tag="PKH60_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012036820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNQ16967.1"
                     /translation="MKGIILAGGNGTRLRPLTLVTSKQLLPIYDQPMIYYPLETLRTA
                     GITDIMIIVAPDHAGDFLKLLGSGKEFGCKFTYEIQDEPAGLAQAFLIGENFIGSDSV
                     TLILGDNIYEDDFSEAITSFKSGGRVFIKEVSDPQRFGVAEFDDQKRVISIEEKPGQP
                     KSNFAVTGLYIYDSTVVKRAKSLVPSQRGELEITDLNNVYLKDNQLDVAFVAGQWFDT
                     GTFESLYEAISFVRSKKIKTE"
     gene            complement(14606..15628)
                     /locus_tag="PKH60_02590"
     CDS             complement(14606..15628)
                     /locus_tag="PKH60_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNQ16968.1"
                     /translation="MIRAVVETVKHLFHPRHSNNHRARLLHSEWLVVFSVMACFSLLG
                     LRFFQSYSEKLGYVLGYASDITVDQTLVLTNQERAKSGLAPLSLNAKLSSAASAKASD
                     MFQNQYWAHVSPTGKEPWSFITASGYSYRLAGENLARDFNHTTDMVIAWMNSPTHRAN
                     IMNGRYTEIGLAVVNGQLQGIDTTLVVQMFGAPSTGSQPQLAQAAAVVEPPAPTATRV
                     PTLQPTAVPLLAQATTSPQPSPMLTPAIVQGQTQLLPPRTEVLSRQIFPSGSVRPLDH
                     INPLSLMKAFFISILVVLMFTLVYDLYAIGHYHSLRIVGKNFAHLLFFAAVAFLLIFF
                     KSGAVI"
     gene            complement(15678..>16205)
                     /locus_tag="PKH60_02595"
     CDS             complement(15678..>16205)
                     /locus_tag="PKH60_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16969.1"
                     /translation="SQRIVATQNSKLEIKSDDELSTVFVALTTTGSLHSSTASLSISP
                     DILSDTGELTFWSVDLWGNTESPKTIQFHVVRNQADPPRVIWQHFENQMLYLVVESNF
                     QEDVLVSHYRLLNENGERISLIRHPWVESSTALHQSGAPISLSFLILDSIHAGRVQSV
                     DQFGNESLASNLILF"
BASE COUNT         4748 a         3579 c         3087 g         4791 t
ORIGIN      
        1 atatcgctgt gagctcccca tcaatattac tctgagttga tgttcgtcta ggtgaaattg
       61 agaaattaag acctccccag ccaattcagt ggtgccagtt tttatgccaa ctactcccag
      121 atttttttct agcaattgat tggttgattt caaaacatgt tttctattgc cagtaacatc
      181 cgagatgatg agtagcggct cttttacaat tgccttaatt aaaggatgac tcatggcgtg
      241 gttagaaagt aggtgtaaat ctttcgctga tgaatattga cctacagcat catatccagt
      301 tgggttacga aaactcgtat tttctagtcc gatttcgctt gccttttcat tcattctggt
      361 gataaatgct tgatatccac ccggatattc tcttgcaagt acttcggcgg cttcattgct
      421 cgatacaatt aacaaacttt ttagtagatc ttcaacaata aattgttcgc ctactgtaaa
      481 atcaagtata ttggaccatt gaatatctga gctttgtacc gttaatacag tctctggtgt
      541 aaatgattca agtgcaacca gagcagtcat caatttttga gtcgaagctg gtggcaattg
      601 agtagattca tttaccgaaa gcagaattga ttcgcttgcc aaatcctcaa catagactga
      661 ttttgctgaa attttgggat attctgttac atctgagcca gtaatccaaa ggtcattttc
      721 atgattacta gcaataatgg cttgccaacg ttgagatgct actgggaccg ctggaactgc
      781 tacgagctga gtgggcacaa ttgtttttat tccaagtgat gctctcacct taccaatttc
      841 tgcttgctgt tgaaaaagca gtaaaaccac aatagttact atggctacta attgacttgc
      901 acgaatcctt ggcattcctt gacaatcact tcttattgtt tgtaactacc tgcaatccca
      961 actctttcaa aagtgtatct tctatactac ttggagcgtc catgaccgca gttctacctg
     1021 ttgatgacat cggaaaagcc acaacttctt tgagagattg ttctcccgct aagagcatgc
     1081 attgtcgatc gaaacctaag ccaataccac catgtggtgg cgtaccgact tcaaaagcac
     1141 gcaacatgtg gccaatactt gattcaattt ctgcatctga gtaacccatg gtggcaaatg
     1201 ttgctttgac tatctctggt ctatgagcac gaatactacc acctccaact tcgtaaccat
     1261 tgcaaacaag atcatattga gttgtgacta tttctgaaat tgattcaccc ttgaggtgtc
     1321 gctccaaata ttctggcttt ggcatgctaa atggattgtg cgtaaacgtc cacccacttt
     1381 ttccatccgc aacttcggca ggatcagttg aatcaacttc cttaaaaaag ggaaaatcaa
     1441 ctacccaagc aaatgcaagc gtgccgtcag ttttctctga ttcggaacgc aagtcaaatt
     1501 tgtctttacc aaattgagct atagcatcct tgtatgagaa aataggaaat tgctgatcac
     1561 gaagcttacc tccaacttga gtaacagcct gcttgaccat attctcaatc aaattcatca
     1621 ctccgttttg atccataaaa ctcacctcga gatctatttg agtatgctca aatcctcgat
     1681 cggcacgtag atcttcatca cgaatacatc gagcaaattg aaaatatcgc tccacccccg
     1741 ccgtcatcag tagttgcttg tattgttgtg gactttgagg cagagcgtaa aatttaccag
     1801 gctgaaacct cgaagggact ataaaatctc gagagccttc ttttgttgat ttggtgagca
     1861 ttggggtttc aacctcaaca aatttatttt cgtgcaagat cgttcgccac gcttgaccat
     1921 aatcacttct gagccgaaga tttttttgca ttcgctgacg acgcaaatcg agataccgat
     1981 attgcaaccg aatttcctca gagattgcgt acccatcgtc atctattgga agtggtaatt
     2041 ccttggccaa attgagcact tcatactcat gtacgtcaat ttcgattgac cccgttagca
     2101 aatctgagtt gaccattttt tcaggacgtt gcttcacttc tcccacaatc ttcaagacag
     2161 attctgtagt gacttcaccc attttttgat acccaacaca ctgaaccttt cccgtactgt
     2221 cacgtaaatc aataaatgtc actttaccat ggtcgcgttt tgaatctacc cagccaacaa
     2281 tagtgaccgt ttgaccaacg gctttagtac agtcaaaaat gggtagagtt tgaattttca
     2341 taactcaagt aattatacag aaattatcaa aaactgcaga agacgaatac tgttataatt
     2401 catctggatg tcctctagaa ccagagaaat accaagtgta gataatcttt tggggttaca
     2461 gtattaccac aaaattggtc aattggcgcc cactgatttt actcccccca tgtttgagtg
     2521 gctaaaactt attaaggagt taccacaact ttcggatgtt gagctacacg aatttatgaa
     2581 attcatgatt ttcttccaaa ctcagctgaa tcaaaaatac gaggagggat cctttttaca
     2641 caaaatttat caaatagatc cacaaactat tgtacgagaa attggtgtca ggtcagccac
     2701 tattcaaaat gtcattttgt acatggttaa taagctcaaa caaactacgg agcttgagtt
     2761 tacagccgat taccaaattg ggattaatag gtttttatca gaacaaattg ctctacttaa
     2821 acacgtactt caattacaat aaattgagga aatgatttgt ttctccaggt ttgtttttct
     2881 agtcgaccaa caagtgaaat ttttgtcccc gtttgaaact ttatagattg gtcaccatgt
     2941 ttccaccaaa gtgcttcgat aacagttttt tggttatcaa gctgattcat atttgcaatt
     3001 acaagtttca gatgttttcc ttcatttcca atcgttttta cacccaccac ttgccaatca
     3061 ctgagtaaga atagtggtct gggattagct tgaccaaaag gcgaaagtga ttcaattgtt
     3121 aaaatggtat ctaggtcgat taattctgat tttagtggac aggtgatgtc aatttgcttt
     3181 gctgcattct tgatagtaag tttatttgcc aatacctgta ttttttcact tactttgtct
     3241 acagtagcag gttccagagc aaaaccagcg gccatggcat ggccgcccaa ctccaaaaag
     3301 ctattagaca tttgtcgtaa aaaatcagta atgtggatgc ctgggattga tctgaccgag
     3361 ccttttgcta atgtctcatt gaccgaaatt actattgcag gcttaccata ctgctcaact
     3421 aacctgccag ctaacagacc aataatacct tcgtgaaatt ctgatccagc gataataagt
     3481 atttttgctg cggtttgttc ttgtgctagt tgatttgcgc gcgcgtagag atcttgggta
     3541 atctcttgtc tttctatgtt tgcctcttct agcaagcgcg cgcgctgttt ggcaaattct
     3601 tttgtacccc cacacaacaa ccgaagggca tccataccgt gtgaaagcct tcccaaagca
     3661 ttcaatctgg gtgcaatctg gaagctgacg cccacttcat cgatcttact ttgatcgatg
     3721 ttacccattt caaataaagc ctgtaggcca tgtctctgag tggtttttaa ttgtcgaagt
     3781 ccatgaaata ccaaacttct atttgcctca ataagtggaa cttgatcggc aatagttgca
     3841 attgacacca aatcaagcag ttgatttgta ttagtatcag ctcccggagt ttcagtcgtt
     3901 ttaagtaaat gcttagctaa gaaccaagcc actccagctc cgcacaactg ggtggtatgt
     3961 acgattgcca gggcatttgg ttcagtacta gctttttcat ggtgatcact aataatcaca
     4021 tcaatgccca ttttagacaa tgttttgaga gcctcatgag cgacaatgcc attatccaca
     4081 gtaacaacga gatcgggctt attttctcct atcatgtcgg gaatacttct ttgattcaaa
     4141 ccatacccat gtgtatgacg attgggtata aatggttgag caatcaatcc catctctttg
     4201 catgccaacc aaagtacggc tgtggcacag attccatcgg cgtcgtaatc accagcaatt
     4261 actacttttt ggtccatttt ttttgcctgg attaaccgcg ccactgcttt tttcatttga
     4321 gacgccacaa taccgacgtc atttagcgtt agtgcatttg gtttgggggg caaaagaaat
     4381 ttttcaacat ttttaatacc tctatcttca agcaaccatt tttgcagctc aactacatca
     4441 tttgggcttt gttcatgcat tagatgccat tgagcttttt gactagtcat tctcgcagag
     4501 caggatataa tgagagtgaa tatgctacaa gtaccatctc agctcacctt agctcttgaa
     4561 cccctgattg tgttgcacga attaaacaaa gctggttttg caaactacat tgtgggtggc
     4621 gcagtaaggg atctactact atcaaatgaa aagtggcttt ttattgattt tgatctaaca
     4681 accaacgcat cccctcagca gttgttaaca atatttcctt ttgcctttta tgagaatact
     4741 tttgggacgg tgagtctcac ctaccaacat ctatgggact ttgctggcat agatttggaa
     4801 tatcgaaaag cccgtgagtt ggtagtcact tctcatcaaa ttagaaagac caaccccacc
     4861 ctgattgatt tattgaacgc aactaaaatt cacacttcgt tgttggaaca caccgagaaa
     4921 cctccgctag aacctcaaaa agcagtgcta ccattaatcg aagtcactac ctttcgttct
     4981 ggtgaaaaat atcaaaacgg cgcacggaga cccagcgaag tacagtgggg acattcgctt
     5041 attgaagatt taaaacgtcg tgatttcacc atcaacgctc ttgccttgaa actggatccc
     5101 gattggattg aaaccacttt caagcaattt actacccatc cacccaacct tcagggaatt
     5161 cctcttgatc aatcagtttt tgaattgata gatcgccaca atggtattac tgatttagaa
     5221 tctggtttgg tacgtactgt tggaatagcc accgaaagaa ttcaggaaga tgcgctgcgc
     5281 atgttgcgag caatccgctt tgcagtgcaa ttgggtttta cattagatcc cgaattagta
     5341 gaggctatta agcaacacgc tctcttgcta aaggatgtga gtccagaaag aatccgggat
     5401 gaatttttta aaatgctcgt atcagaccat ccaaaacaag caattgagct tctggatgag
     5461 actcagctac ttgagcaagt attacctgag ctactttctg caaaaggtgt ccgccaaggc
     5521 ggacaccaca ccacagatgt ttggcagcac agtattgatg cattgcaagc ttgtccatca
     5581 aaagacccga ttgtccgact agcaacttta atacacgata tcgccaaacc agctacatat
     5641 gatgacgcca aaggacaacc aaccttttat aaccacgagg tcataggtgc gcgagtggcc
     5701 aaacagatag gtcaacgctt gcgactctca aaaaacgatt gtgatcgcct cttcgttctc
     5761 gtccgatatc acatgtttca ctatcaacca gaaaattctg atgctgctat ccgtcgattt
     5821 atgcgcaaag ttggccttca acatattgat gatatcctgg atcttcgtga ggcggataga
     5881 cttggatctg gagcgcgaaa aactagctgg cgtctggagg aaatgaaaga acgaatggtg
     5941 gcgcaactcc atcaaccatt tgacagtaat gacttagcca ttgatggcca cgatcttatt
     6001 gatcagcttg gtctcaaacc tggtaaatta attggtacta ttctgcaaac cttattggaa
     6061 atcgtgttag agaacccaga aaaaaacact aaggagattt tactagaaat cgcacgagca
     6121 gaattagagc aatccaaaca gaaaaagtct gtcgcgtgat gttatctttt tcgtttctga
     6181 atccaactgg ccatcgaatc ccaaaaaaat aaaaacggaa ccgcaataaa tggactggaa
     6241 tacgttcctg tgattgcacc gatgaggaga gcgagcgcaa agtttttaat tgagtttcca
     6301 cccagtaggt aaagcgccaa taacataatt atgattgtga ctgaattatt tatcgatcta
     6361 ctcagcgtct caattaccgc ggtattggcc actgtacgga atggtaaacg acgaaacttt
     6421 ggccgtaatt ctctaattct gtcaaataca acgatagtgt catgcacgga aaacgacagt
     6481 gtggttaata tgcctgtaac aaaaagggtg tccacctcaa tccccagata ttttcctaat
     6541 aaactaaata caccaagtaa aataagcgag tcgtgaaaca tggcaaaaat ggcagacaca
     6601 ccatacctta attcatgaaa acgataccaa acatataaag ttataataat tgagactaac
     6661 gccaacgctg ttaaagtttt aatcaataac tctcgactta acaccggacc aatagtttca
     6721 aaccgaagtt gttctaaatc tccaaactga tcgcccaact gagtaattgc attttgcttt
     6781 tgttcgtcga taacaaaagc tgacttaatc accacttgat tttctccagt tttttgaaca
     6841 gacactactg gatagttttc gccccaaatt gcagacagtt tttcaacagt tggttcttgt
     6901 tctccagaat aactaacttc caaaactgtg ccacccgtaa aatcaatgga tggacgcaaa
     6961 ccattaacag ctaatgaaat tagccctgga attaaaatag ttccagaaat aaagaaaaag
     7021 agccagggtc gtttaaatag attaatcatt ttttgctttc aaaaataaac gaacaaatgt
     7081 gcgggtaacg acaactccgg taaacaatcc caatactact ccaatgagaa gtgttaatcc
     7141 aaaacccctc acaagaccac ttgaattgag aaaagaaaaa ctcagtggat taatgagcac
     7201 taaggcagtt aaaatcgtcg ccgcattggc atctttaata ctatcccaag cacgaccaaa
     7261 accaagttcc attgcaatgg catgcggttt accacgacgc aactcttctt tcattctttc
     7321 aaaaattaaa atgtttgcat caactgccat tccaattgag agcagtaaac ccgcaattcc
     7381 tggcaaagat aatgttatgc ccgcaacttt atagatagcc aacgtgagaa cggcgtagac
     7441 taccaaagcc atgtcagcaa tgactccttg ccaaccatag agtaacacca taaacactaa
     7501 tactaagccc aatccgataa gccccgcaat gatgctgcga gaaacagcct cttgacccaa
     7561 acttgcacca atagtcctct gctccaaaac agaaattgga actggcaatg cacctgcatt
     7621 gagttgaatg gaaagttgtt ttgcttcatc caaggtgaat tggccagtta ttactgcctg
     7681 accagtgagg atgggtgtag aaatagtagg caacatcaag ggtacaccgt ccaggaaaat
     7741 acccaatgtt tcgccagtat gatctgtggt cactttggcg aagatttctg ccccttggcc
     7801 attgaattga agtgaaattt ctggctcacg agtttgctca ttcaatacaa cggctgccct
     7861 ctgtaaatca ataccgctca aaccaacatc ttcaacctct ggttggagtt gagccgcgat
     7921 tgatgccggc attccttctg ggggcgtcat gcttgacaca agcataaact gaagttgagc
     7981 agtctgaccc acaagactga gggcttctcc tggatcttct actccaggta attcaacaat
     8041 gattctgaat gaatctccca ctcttgcagt ctgtacgagt ggttcagata tgccatataa
     8101 atcgactcgt cgcataatga ccgcttttgc agattctagt gcggtttcac ggtcagctgg
     8161 gtctacctgg gacatatctg cctcaagcac aacttgcatg cctccctgta tatcaagacc
     8221 ttttttaaaa ggaagatctg tagatatttg tttacccaat agtgaaaagt tcactttggg
     8281 tatgcctaac ggaattgaaa aatccttccc tgctacattt attttttcgt acctcagtga
     8341 gggtgcggca atcatgaggg aaatactaaa tgtcattact ataataaaaa aaagtcgcaa
     8401 aaccctcttt ttcattgtga aattcctggt atgcctcgat caacaaaatc tacctcattc
     8461 ccaatgatca tgactctacc accatagaga acatccataa tgaaaggatc tcttcgcgtt
     8521 ttacgaaatt caaattcatc gctactaaaa acagtgtaat taatttctct ttgaattcga
     8581 tcttgctctt gttttacaag cgcttcaagc tccgaaagta caatatcacc cacaaccaac
     8641 atatcaagtt cgcctctttt ttcctttaat ccgccagcga aacggctaga aaacatcaca
     8701 aattgaactg tccctagttt tctgcgtaat cgacgaattt cttttcctaa accagtgcac
     8761 ttggctacca tctgacgtaa ctcttggaag aaagcataac gtggattgag aaaataatag
     8821 agacgattgc ctcgttgctc actttttagc aaaccaaaac caatcattct atcaagctca
     8881 cgacgaacag catttatttc ttccttcact tctctggtaa tttctctcac aaaaaacatc
     8941 tcatttgttt gtgaaaaaaa tagttcgatt attttcactc tcactctaga aatcattaaa
     9001 tcttgaagag atgtcataat ccgccgccca caatactacg tgtgtttccc gagtatatca
     9061 tacgagttac ttctatacac cagatgtatt cacggttgtt gagtcatcga ctactgaaat
     9121 ccaaaccaac cctggttgga aaactaactc cttacccttt gaatcagtaa actggagctc
     9181 agattctcga gtctttttag tccaagtagc agttatctcc tgaccattct gataaaccaa
     9241 agcttttcca gaaccagtgg ttttatacaa catgtgtttt aactcatcaa ttggaccttt
     9301 ttctgcagtg agcagtacta taaccacact ggctgaaatt tgcttattcg tattgaggtc
     9361 cacatgtggt tctccagcaa gggttctggc atacgttttc gtagtagcat caaattgcca
     9421 cttaacagaa tactggttat acccgctcca gaaatcatat gcaattgagg tgattgttcc
     9481 tggagcttca tttgaactag caaacgtcca tccagtgtaa ccttcttgcc aatctttggc
     9541 gggtaaaaca gttctaccaa ctttcatttc tggcgacata ttagtccaac ctcttttctc
     9601 cgcaactgcc catagttttt ctgaggtggt ctccatggtg tgttcagtcg cgacctcacg
     9661 atctagtcga gctgcatttc gaacaaatgt aggataccca atcgaaaatt ggttcagatc
     9721 attttcgagc gcccaaccat aatcagcaat ttgcccaata gcattagcag gaccaggtgt
     9781 attagcccca cctacgtggg tgtacaacgg acgattgaat ccagatgccc aatcaacaaa
     9841 ataagtcctg gctgaacgca cgggagcaat tgttgtatct actgcttgca ccgcacagta
     9901 atacatagcc ataaatcgag tcacacctcc ctcagctact gcttcaaaaa ccacatcggc
     9961 acttgatagt ccagactgtg gtcttgcatc tggagaattt tctatcataa ctgccaatgg
    10021 tcttcttgcc tgccaagcgg ctgcttcggt tttggtatat aacttcccgt tgagtgggca
    10081 cgattgatcc tttggttctg atgggtctat agataacaat gcaccaagcg cagtctcttc
    10141 ctcaaacgtt ggtacagatt gaccatcgga attgatgctt gctgggcttg tatcaaacat
    10201 cgctctgaca gcataggtac caattgccga tccaatgtac agcaccagta aaatgagtaa
    10261 gaattgtttg ctcttcataa gtcctttcag aaaataggca gtcaatctac attaattgta
    10321 ccgaaaatac acaaaaatac caagtaatag tactacgaga ataagtgtga tagcaatgct
    10381 tctaaacaga tctctgtaaa ggagtttaat gggatatccc aagagagttg ttgtatctat
    10441 ttcatgagta tctaggtttg ctcgatccat tgggtctaca ccttgctttt gggttactgc
    10501 tggagcactt gcccgatgtt gtttagcgct aatctttgct ttctttgttc taatctttgc
    10561 catattagcc acccatgata atcctactaa tatcactaaa agtaattaaa accaccaatg
    10621 ctagcaaaaa cacatagcct ccgtagttgg cgtaatattc aattagatga gatttatttt
    10681 tcccaatgag taattctagt atactcaata ctgcccttcc accatctaac ggtggaaacg
    10741 gcaatatatt catgattgct aaattgatag ataaaatacc ggcaaaagtg agaatgctta
    10801 accaaccttg atcaaagaat tgcccttttt gagcctgata aacgattcca accggaccag
    10861 caacatctga agagaccttg cctctaaata agtcttgtcc aactccgctg agggcatcga
    10921 gaatcatttt tgaaagctgt agggcttgta ctgacccatg ccaagcactg cggaaaggca
    10981 tttcgtacca tggataggat tgaaaaataa cagtttcaaa cgaaatgccc aatgagcctt
    11041 ggttttcggg ggtttcatca gctgttcgca gatagacaga gtattcttga cgatcctcgc
    11101 aaatcaattg ttgacaaatt ccagttgtga tcaatgtaac ggtttcgcct cgattttctt
    11161 caacaaatga tttcaccgat tctactgttt tagtttcaac cagcccatca tcatccagaa
    11221 atccaattat ctggacttga tttggcactc ctgcctgagc cgctggtgaa ttaggctcaa
    11281 ccgtaccaat tctggcccca gagatttctt gaggaatacc tgttttgcta aaaacaatag
    11341 taaaggcagc tacaccaaat ataaaattaa ctgcaactcc agcaaatatg accattaacc
    11401 gttgggcgag agttgcagct agaaaatcgc ccggctgtgg tgtagcgttt tgtttggggt
    11461 ctggagtctc tccggccatt cgcacaaatc ctccaaaagg aatccaattt aaagtaagct
    11521 cggttcttga tgaatccaac ctacgccaga aggaaaaata ttttgtaatg ttgaaccgct
    11581 tggctaacag tgaaaaattg agcgcacgtg ggggataccc aataccaaac tcctcgaccg
    11641 taatacctgc ttttctggct gcaaaatagt gaccaagctc atgaataatg acgaggaatg
    11701 atagtactaa taaaacaatc aaaattgcca tacagcaagt ccgctatctt tatacgtgaa
    11761 gtaattcctt ttccttttgt gcttgcagtt cgtccagttt tgtaagcgct agctttactt
    11821 ctttatctaa ttcatctagt tcgagcttga tatcatcctc actgactcca gctccgccct
    11881 tttgctcctc gatttcttgc tttgtttctg tgcggatcgt acgtacagtc actcgtccag
    11941 actcgatttt ttgatgtaac aacttcacca tttcctgtct tctttcttgt gtgagagcgg
    12001 ggacagctat acgaataatt tgaccatcaa ccaccggatt taaattaagg ccggcgctgg
    12061 caatggcttt ttcaattgaa gtaatcaaag acttgtccca aggtgaaaca acaagtaatg
    12121 tagcatctgg agcagttacg gaagcaactt ccaccaaact catctgagta ccataggcct
    12181 ccaccctcac cggatcaagt aattgaacgg atgctcgccc agtccgcaaa cttctcacat
    12241 cttgaagcac gtgctcataa gatttagaaa ttttttgctg gaaatgattt attgcatctg
    12301 tcataggttg attgtaacag acactactgc ttctttggct catctaatac aatctcaaac
    12361 tcaacttggg gtaaagctag ttcagcttct ctaacatcaa ttgcctcagt tcgttgtttt
    12421 gcttcaagaa tgcgactaag taatggggtt aggattggtg tctcagttgg agttggagtt
    12481 ggggccacca cctccacaat ctcaggctca ggtcttgaaa gtagattaac catcagtaat
    12541 atgattgcaa ttatgagtgc gattgagcca acactaatca taataacttt tcgctgtctg
    12601 gtttttttct cagctcgttc tagttgttct gcaggcgtga gactgggttc ttctagccat
    12661 gtttgacttg cagtaaattc cgtttgctga atctgtggtt cttttgaaaa taatttcata
    12721 actgagctgc ttgttctagc aaagtgtacc aacgaatcaa gcctgattgc agtggactaa
    12781 gggtgacaat aaatgctgtg taggcttggt tggagaaatc ttcttctgtt tgatactgta
    12841 gtactgattt ctcccatgca gtctgaacaa cagcatcagc cgacgttaat tcttgatagg
    12901 ctcgctgtcg ctcgccttgc ttcagagcag acacgggatt tgcctcatgt gaggccttaa
    12961 tctcttgagt tagttgttgt gccatccctg cagaagatcg aaatttacca agaaataaca
    13021 gctttctggt ttggactgaa atggattgca attcagttcc taagggtatg aatcctaccg
    13081 ctctctccgc aagtgcttgt ctatcttgac tatttatcac caactgatta aacgttacca
    13141 actcagctcg aagctgttta attttattta aatactcctg gcggatttct tcgtcataac
    13201 ttggattgac tgttaattct aaaaataact gatccaggta ggtgattaaa acaatatttc
    13261 tggcactaaa gacttctctt gtggatagta ccgcctcctc cagtgaagcc agagttgcta
    13321 ggttttgaaa ttgagttttt gccaatgaaa attttctgta actattctga tacgattgaa
    13381 tctgatctct atacaaggct tttaatgctt gtcgctcatc ttcgatgtct ttttcgacag
    13441 tagtttcttc aacagattct tctatagttg taacggttgg agtgagttct tcggtttgtg
    13501 ccgtaacatg agctgcgcgt aacaaaccaa ctaggaatat taagctaaag agtactacta
    13561 gtcgtttcat tctgtcttaa tctttttact gcgtacaaaa gaaatggcct catataatga
    13621 ctcaaatgtt cctgtgtcaa accattgtcc agctacaaaa gcaacatcta gttgattgtc
    13681 tttgagatac acattgttta gatcggtaat ttctagttca ccacgttgtg aaggcactaa
    13741 actttttgct cgtttaacca ctgttgaatc atatatatac aaacctgtca cggcaaaatt
    13801 ggattttggc tgtcctggct tctcttctat agaaatcacc cttttctgat catcaaattc
    13861 tgccacacca aatcgctgcg gatccgacac ttcttttata aacactcttc cacccgattt
    13921 aaagctggtt attgcttcag aaaaatcatc ttcataaata ttgtcaccca agattagcgt
    13981 cacagagtct gaaccaataa agttttcgcc gattaagaat gcttgagcca accctgcggg
    14041 ttcgtcctga atctcatagg taaatttaca accaaattcc ttaccactac ctaaaagttt
    14101 gagaaaatca cccgcatgat ctggagcaac aataatcatt atatcggtaa ttccagctgt
    14161 tctgagtgtt tctagtggat aatagatcat tggttgatcg taaattggga gcagctgttt
    14221 gctggtaact aaagtgagtg gacgcaaccg cgtaccattc ccaccagcca aaattattcc
    14281 cttcatatac agacctttct gtgatcgatc gtaattttat tcattgtaac attctattta
    14341 atattgaact aaaaagtaat gaaccaaagt aaaagtaccg ctagcattga tataaataca
    14401 tactcgccta tgacttgccg agttatgcgt ttacttttgg tatgtgcaag aataccccat
    14461 gtagcataaa agagtccaag tgcgagtgaa atatatcttg tcatccaaac atttggccaa
    14521 aaaaataagt aaagataaac aaacgcacac aagcctgcaa cgagtgctaa gtaggatcgc
    14581 cagtgactac gaatttcttg ccacattaga tgactgctcc tgatttaaaa aatattaata
    14641 aaaatgccac tgcggcaaaa aataataaat gagcaaagtt tttaccaaca attctcaagg
    14701 agtggtaatg tccaattgca tataaatcat atactaaagt aaacatgagt acgacaagga
    14761 tgctgatgaa aaaagccttc attaaggata gggggttaat atgatcaagc ggacgaactg
    14821 atccggaagg aaaaatctgc cttgagagaa cctccgtcct tggtgggagc agctgtgttt
    14881 gaccttgcac aatcgcaggc gttagcattg gagaaggctg gggggaagta gtggcttgag
    14941 caagtaaggg aacggcggtg ggttgtagag tgggtacccg ggttgctgtt ggagctggtg
    15001 gctcaacaac tgcagcagct tgagccaact gtggttgtga tcctgtactt ggagccccaa
    15061 acatttgtac taccaatgtg gtatcgattc cttgtaactg cccattcaca accgctaaac
    15121 caatttctgt atacctacca ttcataatat tagctctatg tgttggtgag ttcatccagg
    15181 caataaccat gtctgtggtg tgattaaaat cccgagctaa attttcacca gctaatcgat
    15241 atgagtatcc cgaagcggtg ataaaagacc atggctcctt gcctgttgga gaaacgtgag
    15301 cccaatattg gttttgaaac atatctgatg ccttagcgct cgctgcacta gatagcttcg
    15361 catttagaga aaggggtgct aatccagatt ttgcacgttc ctgatttgtc agaactaatg
    15421 tctgatctac agtaatgtcg gaagcgtagc cgagcacata acccagtttt tctgaatatg
    15481 attgaaaaaa acgcaatccc aacaaagaaa aacaagccat cacagaaaaa accactaacc
    15541 attccgagtg cagtaaacgt gcacggtgat tgttggaatg gcgcggatga aaaaggtgct
    15601 tgactgtttc aactacggcg cgaatcacaa ctccattatg caaaatagaa ttggtaagag
    15661 caagtggcga ccgacaacta aaacagaatc aaattgctag ctaatgattc attgccaaat
    15721 tgatcaacag attggactct tccagcatga atactatcca agatgagaaa actcaaacta
    15781 atcggcgctc ctgattgatg tagcgctgtt gatgattcta cccaaggatg cctgatcaaa
    15841 ctaattcttt ctccattctc attcagcaat cgatagtgac tcactaaaac atcttcttga
    15901 aagttgcttt caactacaag atacaacatc tggttctcaa agtgttgcca aattactctc
    15961 ggtggatcag cctgattcct taccacatga aattgaattg ttttgggaga ctcggtgttt
    16021 ccccataaat ccacagacca aaaagttagt tcacctgtat cggatagaat atctggacta
    16081 atcgacaacg atgctgtact ggaatgaaga ctaccagtag tagtaagcgc aacaaacaca
    16141 gtggatagct catcgtcgct ttttatttct aacttactat tttgagtagc aacaattctt
    16201 tggga
//
LOCUS       DAOQZP010000024        15861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_4123_length_15861_cov_5.607997, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000024 DAOQZP010000000
VERSION     DAOQZP010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 15861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15861
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4123_length_15861_cov_5.607997"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..765)
                     /gene="lexA"
                     /locus_tag="PKH60_02600"
     CDS             complement(145..765)
                     /gene="lexA"
                     /locus_tag="PKH60_02600"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNQ16970.1"
                     /translation="MAVTLYKRQRQIVDFIAQYIQKNTYSPTLKEIAQAIGVSSLATI
                     HEHLQALERKKVIRKQDGQVRGIELVEKTFLSTSESVDLPILGYIAAGSPIEPHTDPN
                     ASFKVSPELISGKRRSYVLQVKGQSMIEDHIDDGDYVVIEETPSVNDGDIVVALLDNG
                     LATLKRFYKEVTRVRLEPANSSMSPIYATNVQIQGRVVGLIRRYQQ"
     gene            complement(918..2195)
                     /locus_tag="PKH60_02605"
     CDS             complement(918..2195)
                     /locus_tag="PKH60_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase"
                     /protein_id="HNQ16971.1"
                     /translation="MATIQGLWAREVLDSRGIPTIECTLWLDTGGIVAASVPTGSSIG
                     KYESVEVRDRDQNRFLGKGVTQAVQSINTQIAPQLIGKDPTSQQEIDQLLINLDGTAK
                     KEKLGSNTLLAVSQAVIKGGALSLNQPLYFYIQSKYKMSQTLKIPTCIYTMVNGGEHG
                     ANNLDIQEFQIIAASHLDFSNSLNMAVTMFRHLSQVLAAKNAIHSVGPVGGFTPNLYN
                     NTDVFELLIETAKTTSYVFSQDFFLGVDAAASKFYTGGKYHLRERTEGYSSTELLEYY
                     KKIYDVFRVLCFEDPFREDDEKMWQQITAELGETSRIVSDLLTATNPERTQKAIDKKM
                     ANTLLVKPNQIGTMSETIAVIQLAKKAGWQILVSHRSGETNDDLIADFAVGIGADYVK
                     FGPPNRGERTAKYNRLLMIEAELRRSQLATETK"
     gene            complement(2198..3973)
                     /locus_tag="PKH60_02610"
     CDS             complement(2198..3973)
                     /locus_tag="PKH60_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16972.1"
                     /translation="MSILPIVVRVEQFADFSQANLGDRALDLAWLRQFVSVPPTLLIP
                     ADTFDKVLISTHSVLKLQQLIDDDQMSPTSKQKKISEFFTKLLLPQSFITHIHAEYHE
                     YFSNQPVRVFSATSSAGSTLNEVRGEVNLLISIKKIWGELILNSIKQSKKHHQLVPPN
                     ILIQAIPITKVVGFVASHHLGGMHKAALVIQAQAGAGKKEGDIETYQVDVRSQAIIVR
                     PDIQPQPRNNKKIELSLLSDAECRQLAALSIPIIRQRLRPMVIHWSLTQAGFVFYDLL
                     QPSQTNTSHPATQTQPTSATKVYVASNSIARMSASIPLVDGVGPISARYLVHASGLHP
                     IGALRNTSTQTVLKHNIRRALFELVSVKRDLTILYSLYTATPTQRAKLFLSESERTAE
                     AETAMLSGASFLLTFPQWLDFELQTLVEFLKQTPIHLDLVLPEIKTPAEIPLLLKHIQ
                     KSGLQKMPNVKIWLEVATPAIAQSLHLFPLDNISGILINLNELLPRLLCVDPAMIRVK
                     KESTQQLATAIIQDLLSGVSKQLLLSFHTQQIGVMVLSQIIEPIVVKKIVSLGWQGIC
                     VQPSQILETRSYIQNAEKLVIEHKL"
     gene            complement(4015..5100)
                     /locus_tag="PKH60_02615"
     CDS             complement(4015..5100)
                     /locus_tag="PKH60_02615"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNQ16973.1"
                     /translation="MTPSSVDLNDRKSIAKLDQSNMLGSIEALGKQIEHAWQETRAIS
                     IKPATPITQVVIAGMGGSGLGADVIKHLYTEQLTVPLDFIHGYSLPAYVNESTLVILS
                     SYSGNTEEILECAKQAQAKKSTIACIAAGGKLVALAKKHHWPLYEIKPTHNPSGQPRM
                     AIGYSVIGTIGLCERAGVVSISQSEFDTTTELISNLCREYAVEITQDQNFAKLLAFTM
                     FNKQTVLIAPDFLEGAIHVAANQSNENGKTFTTYFVIPEMNHHLLESLRYPNSLQDTH
                     VVLFFQTLLANSSNQIRVNLSQQIFEDHEIETMLIALKGDTKLAQVFELITMMAFATF
                     YQCMLEGINPSPIPIVESFKELLKKNR"
     gene            complement(5109..5645)
                     /locus_tag="PKH60_02620"
     CDS             complement(5109..5645)
                     /locus_tag="PKH60_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNQ16974.1"
                     /translation="MKIGVISDTHDHFPGIDWAHQVLVKASVDAICHCGDWVAPFTME
                     YFVSAFSDFKVPVYSVFGNNEGDLKRILERNQQLPNPITFSPKTMFPLLFDGKKIAIY
                     HGHDSDLLEALITSRLYDAIFTGHTHQYRNEVRGKTLILNPGSLIPARLSKPSGVVSL
                     AIYDTTSNTATHHSINVD"
     gene            complement(5642..6421)
                     /locus_tag="PKH60_02625"
     CDS             complement(5642..6421)
                     /locus_tag="PKH60_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HNQ16975.1"
                     /translation="MTNYQNHRLFAEKLVQEGGAIALRDFNQQKFSTEWKSDHSYVTQ
                     TDTEIETRFRSLIATEYPDHSIYGEEYGGSLDPSRPTWILDPLDGTTNFSHHIPLFCS
                     MAALQIDGKIVAAAVSSPIQDLIFSASLGAGATVNNKPFPNPSQETELDSTTLLLDSG
                     KSTNARSDSFHFLATCGHQFRSFRKFGCMVAPLLYAIHSKLEAAIIFGVDLYDIAGLS
                     LIMSEAGYTTLGKTGNEWRLTENSDFIVTTSALKSKIEVLV"
     gene            complement(6439..7857)
                     /locus_tag="PKH60_02630"
     CDS             complement(6439..7857)
                     /locus_tag="PKH60_02630"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNQ16976.1"
                     /translation="MYKIVKINPVIFRGYDLRGVVDVDLNDDVYYTLGLGYATYLSKW
                     RIKQVAVGRDNRLTSEQYSKAFRTGLNQGGVDTIDLGHSLSQIVYFSSYFFQTKGGAM
                     VTASHNPSNFNGLKLSTGYSETMVTEEIQELRLICEKAEFSQGTGTDKKQDIFTNYTE
                     SILKHFSLQKRWKIVVDACNTTSGMFYPEIFRQAGCEVIEQNCTLDGNFPLGVPDPTE
                     VEVLNRLAEGVKKHKADIGFAYDTDGDRMAVVDENGKVLWMDTIVALFAQDVLDFLPG
                     EKIVYNALCSRQVTEAIENAGGKPVVWLTGHSFIKAKVKEEKAPFGGELSGHIFFMDN
                     FFGHDDGAFASLRLLAYLERKKETLSEAVGKLSQYVSSPEIKFGLADAIKFEFVSTKI
                     RAAFEKEWPQAQYIDIDGIRMDTKETMAIVRASQNGPYITLKFEGKTQAQYDELKRTL
                     KLILEAYPEIDWKQGVNTHALQ"
     gene            complement(7926..8609)
                     /locus_tag="PKH60_02635"
     CDS             complement(7926..8609)
                     /locus_tag="PKH60_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16977.1"
                     /translation="MYIFHGDNHIASRQALREYIESSRERFTRTTTYLATDLTLAQLE
                     SALGEVSLFNEQVLTVVEELHSLPKSTHQTALLNALTKSQTIESIILWEKKLLTPNQL
                     ATFSQAKQKTFKSSKSLFQWLDVFSPSTSIQTKVKLFNTAVAQDGIEYCFLMLVRQIR
                     LLLTIKGGGTIAGPPFVQAKIQKQSKMFSLQQLLMLHEKLTCIDVESKTSGLQLSLKN
                     RLDLLQIEL"
     gene            complement(8620..9279)
                     /locus_tag="PKH60_02640"
     CDS             complement(8620..9279)
                     /locus_tag="PKH60_02640"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNQ16978.1"
                     /translation="MDAVITIQVQPKARRIKLQIVNDGEVLVTTPPHLPKFIINRFIA
                     DHQEWINKHSIKHKGKLKVLTTTYIYLFGTKYDLIYSNDTADSPGFVLTQDKLLYTPI
                     AGKTIQMNHKLLNNFLKSTCSAYILPRTHQLAKNMNVSFKTIGFKEQKTRWGSCSSRG
                     TLSFNWRLVHFEPSVIDYVITHELAHLVHPNHSRAFWKLVSEHFPNLASAKKTLQQYY
                     W"
     gene            complement(9299..10264)
                     /locus_tag="PKH60_02645"
     CDS             complement(9299..10264)
                     /locus_tag="PKH60_02645"
                     /inference="COORDINATES: protein motif:HMM:NF019626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1704 domain-containing protein"
                     /protein_id="HNQ16979.1"
                     /translation="MSLISRITPTNVTAQKLAFLENPNFNPLLTYDEPLGSEELVQFG
                     KPENKYLELAKAILEKAYWHRNEADLNALEGTLCTQSQVEASIFAFLKLHQLENRFKV
                     SFSHSYLSRASISATLLKIRLPLDFRKDGLFSALYHEIGTHALRRLNYEEQPWFKKKS
                     KYGFQSYLVTEEGLASLHGLLPQSFQVAHVAALRYLVVSWAQSYSFLEVWHKLGRYIQ
                     DPGRRWVIAIRQKRGLEDTSQPGGFTKDAVYFQGMVEVWRWLSQHDFNPSDLYWGKLH
                     HEDVELAKSLKPDFQPKLPSFFASNPKKYQKQLKEIGELNYFTQI"
     gene            complement(10261..11547)
                     /locus_tag="PKH60_02650"
     CDS             complement(10261..11547)
                     /locus_tag="PKH60_02650"
                     /inference="COORDINATES: protein motif:HMM:NF025755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HNQ16980.1"
                     /translation="MPIKPSHILGMNGRYRYTKLNPSSARAFGFSKLKTKQLLLENNI
                     PTAQLYHMFESYTDLDHVIWENIPCPFVLKPASGSAGKGIRIITKYLPQRSIWQDNEN
                     EEVTVEDLNLHVQNILDGEYSTWGSSHRALVEEMIIAHPVLKKLSKGGTPDIRIIVFN
                     SVPIMAMARIPTEESGGKANLDIGAIGLGIDMSSGLTTFGIQGKSKYIKRFPQQKRKV
                     SGIQIPFWRETLLMAVSAAQAAGFIFMGADLFIDETKGPMVVELNGFPGLSIQLANKA
                     GLKRRIERVEGIEVRNADHGVRIAQALFAKFYVDQQRVEQGLTIISNKPTIVVKGDRT
                     KDLEVPALVNTLRARSAISRKLAVDLGLLDYDDLLWQQQEVSEGKVPVVEVEFEVKDQ
                     LVKTAMLAVQRLDKSKYKVELGQHDIKQFLIGDIET"
     gene            complement(11553..12533)
                     /locus_tag="PKH60_02655"
     CDS             complement(11553..12533)
                     /locus_tag="PKH60_02655"
                     /inference="COORDINATES: protein motif:HMM:NF025760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM domain-containing protein"
                     /protein_id="HNQ16981.1"
                     /translation="MKETARMKHFVFSILLILSLFFHFNVNFVERASAQTNPSEVVTG
                     EIILSNTEASDSASATAAGEIASDSAEIIEKIQEKKDQDLTDTGGKQIGELTAYLYQN
                     PPEPLSWNNFLQHAIFNTVKEGVPANIIVVVILFPVVASLIAASRHVIGLKGFGIYIP
                     AVLSVAFVSTGVIPGLLMFMAISGTAFITSRLLKQTNLPYLPRTALLLWTISIGIIGL
                     LILAPILKVTQLMSVNIFPILILVLLSENFLDAQAHSKESQALALTVETIILAIVASV
                     LFKWGTLQRIALSEPELLLFLTAVVNIIIGKFSGLRVSERLRFGAIIEEE"
     gene            complement(12606..12962)
                     /locus_tag="PKH60_02660"
     CDS             complement(12606..12962)
                     /locus_tag="PKH60_02660"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNQ16982.1"
                     /translation="MLRPFIKTLLAIFILAWFLPNISYTDWVALGLFSVVLTLINLIL
                     KPILKLLTLPINIVTLGLFSVVVNVALLWLALYLVPSFTIAPMVLFGVPLGEFFTLVV
                     ISFALSLTQSFVSIFL"
     gene            complement(13016..14308)
                     /locus_tag="PKH60_02665"
     CDS             complement(13016..14308)
                     /locus_tag="PKH60_02665"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNQ16983.1"
                     /translation="MLVSAPSSPGVYWFSDIDGKPLYVGKAKNLKRRLSSYTQLKRLP
                     QRILQMVKAAQQVQYQELPSELAAILTEAELIRTYQPSYNILLKDDKSPLYIYITSDK
                     VPQVIQIRKKQLVFNTTKPNIFGPFASSYQVQQVLKIARAIFRWCDDPTNTAGCFYSH
                     LNLCSGVCCGKITLAEYQTDLKQLSLFLHGKSGDLLNQLKLSMKLQSKNNLFEAAAKT
                     RDMIKAITAVTKEPYYLKPDPQLLNLADSRPDNQLTYLSRLLQLYLGLPKHYSLKKIE
                     GYDVSNTSGTNAAVALVSFTDGQAEPADYRLFNIRTLDTPNDYQMLREAIIRRQNHSE
                     WGIPDLVVIDGGKGQLRAALGVWQWSTPMISIAKRPDRLLFPTIDRSSNRLKISYTTV
                     NLDSSHPGLQLVQQIRDEAHRFSKKQHSRLRTRSLLGK"
     gene            complement(14202..>15861)
                     /locus_tag="PKH60_02670"
     CDS             complement(14202..>15861)
                     /locus_tag="PKH60_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNQ16984.1"
                     /translation="NEGSPLEPQIDKTAEKTSISFDFPNEIVGEGKKRNFSIQFSSPV
                     FATLNGKVLEITIPKTQQVGAYTKSSLIVRVPKEFGQPYRIDPSPTTIRDLAGWSEFV
                     YQNYSGQGITAVFGESQRYQLDLTYYLDNPQNYKVSTSLTLPPDTPYQRMLYTNLDPK
                     PENVTLDEDGNWLAEYYLEPLEQKVVTAQAFTELSVNPKHKQLTINESKFTQPLEYWE
                     TQDPAIVSLANQYKSPQAIYEVVVDKLDYTELPLTNSRTRYGAAGSLQNAQDAVCQEF
                     TDLFIAIARSNDIPARRATGYAYTNQPELRPLSLDADVLHTWPEYYDEVNQIWKPIDP
                     TWEDTTGGVDYFNFFDLNHIVFAYNGTSSSQPPPAGAFKPLESTEQTAIVTVTSEFPE
                     ITSNYSATFENKQMYGAPIPGFFQVAIENQTGKAWYNTSVGIRGQKNQVAVQWLSQDN
                     FPEVFLPFATEKRLIFLTSDQLLPVKDTIIVAITNQSGPTQYVNSETPVIILPYYLAR
                     IEFYFGVGIAVSLCALLTGSLLVFRHRRQTVIRRESQEPQTPIV"
BASE COUNT         4426 a         3045 c         3411 g         4979 t
ORIGIN      
        1 gttgcattat cacaacagga aaatgttaga ccggtggtat ttcaatacct taatcgcttc
       61 tctgactatc tgtacatcct tcgttgtttt gttaaccact cccttgagta tcaggaaaaa
      121 cagtttgttc gcaacgagag ctgattattg ttgatatctt ctaatgagtc ccaccactct
      181 cccttggatc tggacattgg tagcataaat cggactcatg ctggaattag caggttcaag
      241 tcttactcta gttacttctt tgtaaaatct tttaagtgtg gccaaaccat tgtctagtag
      301 ggcaacgaca atatcaccat cattgacgct gggcgtttcc tcgataacaa catagtctcc
      361 gtcatcaata tgatcctcaa tcattgattg acctttaact tgaagtacgt acgatcttct
      421 tttaccactg atgagctcag gagaaacctt gaaagaggca ttgggatcgg tatgtggctc
      481 aatgggagat ccagctgcaa tgtagccaag aattggcaga tcaacacttt cggatgtgga
      541 gagaaaggtt ttttcaacta gctcaatccc tctaacttga ccatcttgtt tacgaattac
      601 tttttttctt tctagagcct gtaggtgctc atggatggta gccaaagagg aaacgccaat
      661 cgcttgagct atttctttta aagttggtga ataggtattt ttttgaatat attgagcaat
      721 aaagtcaaca atttgacgtt gacgcttgta caaagtgact gccatactac ctcctatttt
      781 aattaactta tatatactag ttacgttata aatttggcta acaattacca aactacccac
      841 acagtacccg aatatagggt gctcgtgcaa tgggtgaaaa tatatcaaaa tagataagaa
      901 atggaaaaag cctaaaatta cttcgtttca gtggctaatt gactacgtct cagctcagct
      961 tcaatcatta agagccgatt gtactttgct gtacgctcgc ctctatttgg gggtccaaat
     1021 ttgacgtaat cagcaccaat accaacggca aaatctgcta ttaaatcatc atttgtttcc
     1081 ccacttctat gtgaaaccaa aatttgccaa ccagcttttt tggctaattg aatcaccgca
     1141 atggtttctg acattgtgcc aatttgattt ggtttaacta gtaatgtgtt ggccattttt
     1201 ttatcaatag ctttttgagt tcgttctgga ttagtcgcag tgagcagatc cgagacaatt
     1261 cttgaggttt ctccgagttc tgcagtaatt tgttgccaca ttttttcgtc atcttctcta
     1321 aatggatctt caaaacaaag gacgcgaaaa acgtcataaa tttttttgta atattcgagc
     1381 aattcagttg aagaatagcc ttcagtgcgc tctcttaaat gatatttgcc gccggtataa
     1441 aatttactcg cagcagcatc gactccgagg aaaaaatctt gactaaaaac atatgaggtg
     1501 gttttggcgg tttctattaa gagctcaaat acatcggtat tattgtacaa attgggagtg
     1561 aaaccgccaa cgggtccaac cgagtgaatg gcgttttttg ctgccaatac ttgactcaaa
     1621 tggcgaaaca tagttacagc catattaaga gagtttgaga aatctaagtg tgaagcagca
     1681 ataatctgga actcttggat atctaggtta tttgcgccat gctctccccc attcaccatg
     1741 gtatatatgc aggtaggtat ttttagagtt tgcgacattt tatatttgga ttgaatatag
     1801 aagtagagtg gttggttaag gcttaaagca ccgcctttta ttaccgcttg agataccgcg
     1861 agaagcgtgt tggagccaag tttttctttt ttggctgtgc catcaagatt aatcaatagt
     1921 tggtcgatct cttgctgtga agttggatct ttgccgatga gttgtggggc aatctgtgtg
     1981 ttaattgatt gcacagcttg cgttactcct ttgccgagaa aacgattttg atctcgatcg
     2041 cgaacttcta ccgattcata tttaccaata ctactaccgg ttggcacact agcggcaaca
     2101 atgccacctg tgtctaacca caaagtacat tcgatggtgg gtattcctct agaatccagg
     2161 acttctctag cccagaggcc ttgtattgtt gccatagtta taatttatgt tcgattacga
     2221 gtttttctgc attttgtata tatgatcgag tttcaaggat ttgacttggt tgaacacaaa
     2281 taccctgcca accaagacta acaatctttt ttactactat tggttcgatg atttgtgata
     2341 aaaccatcac tccaatctgt tgcgtatgaa aagatagtaa taattgtttt gatactccac
     2401 ttaggagatc ttgaatgatt gccgttgcta attgttgagt actctctttc ttcaccctta
     2461 tcatagcagg atccacgcag agtaatctag gtagtagttc attgagattg attaaaattc
     2521 cagaaatatt atctaatgga aagagatgta agctctgagc aatagctggt gtagctactt
     2581 ctaaccaaat tttaacattt ggcatttttt gcaatccact cttttgtatg tgcttaagaa
     2641 gcaaggggat ttcagcaggt gttttgattt ctggtaatac cagatctaaa tgaattgggg
     2701 tttgttttag aaattcaacg agggtctgaa gctcaaaatc cagccattgc ggaaacgtga
     2761 gtaaaaagct tgctccagat agcatagccg tttcagcctc cgctgttctt tcactttctg
     2821 aaagaaataa tttggcgcgt tgagtgggcg tagcagtata taaagaatag agaatagtta
     2881 gatctctttt aacagacact aactcaaata aagctcgacg gatattgtgt tttaaaacag
     2941 tttgagtgga agtatttctg agggctccaa ttgggtgaag accggaagca tggactaaat
     3001 atcttgcaga aattggacct acgccatcta ctagcggaat tgatgctgac attcttgcaa
     3061 tactatttga agcgacatat accttagttg cggaagttgg ttgtgtttga gttgctggat
     3121 gggaggtgtt tgtttgactt ggttggagca gatcgtaaaa aacaaaccct gcttgagtaa
     3181 gcgaccaatg gatgaccatt ggtcgcagac gttgacgaat tattggaatg ctgagtgccg
     3241 ccagctggcg gcactcagca tcactgagca aagaaagttc aattttttta ttatttcttg
     3301 gttggggttg tatgtctggc ctcacaataa tcgcctgact gcgaacatca acttgatatg
     3361 tttcaatatc tccctctttt ttacctgctc cagcttgagc ctggatgacg agcgccgctt
     3421 tgtgcatgcc gccaaggtgg tgagaggcta caaatcccac tacttttgtg atcggaatcg
     3481 cttgaattaa aatatttggc ggtactaatt gatggtgttt ttttgattgt ttaatagaat
     3541 ttagaatcaa ttccccccaa atttttttaa tagagatgag tagatttact tcaccacgga
     3601 cttcattgag agtactaccg gcagaactag tagcagaaaa tactcgtact ggctgattgg
     3661 aaaaatattc atgatattca gcgtgaatat gcgttatgaa ggattggggt aataagagtt
     3721 tggtaaaaaa ttctgaaatt ttcttttgtt tggaagtggg actcatttgg tcgtcatcta
     3781 tcagctgttg caacttgagt acgctatgag tactaatgag taccttatcg aaagtatcag
     3841 ctggaatgag tagcgttggc ggaactgaaa cgaattgacg taaccaagct aaatcaagtg
     3901 ctcgatcacc aagattggct tgtgagaaat cagcaaattg ttctactcgg acgacgatgg
     3961 gcaggatcga catgtagtaa tcataccatt tactctaacg aataggcaga ttgactatcg
     4021 atttttcttt agtaattcct tgaaagattc tacgatcgga atagggcttg gattgattcc
     4081 ttctaacatg cactggtaga aggtggcaaa ggccatcatt gtgatcagtt caaaaacttg
     4141 agctaatttt gtatccccct tgagcgcaat gagcatagtt tctatttcat gatcttcaaa
     4201 tatttgttgc gaaagattaa ctcttatctg gttgctgctg tttgcaagca aagtttgaaa
     4261 aaatagaacc acatgagtat cctggagact attggggtag cgcagagatt caagtaaatg
     4321 atggttcatt tctggaatta caaagtaggt ggtaaacgtt ttgccatttt cgttactctg
     4381 attggcggca acgtgaatcg cgccttctaa aaagtctgga gcaattaaaa cagtctgttt
     4441 gttgaacatt gtaaatgcta gcaactttgc aaagttctgg tcttgagtaa tctcaactgc
     4501 gtactcgcgg caaaggttag aaataagctc ggtggttgta tcaaattcgg attgagaaat
     4561 tgacacaact cccgctctct cgcacagtcc aattgtaccg atgaccgaat aaccgatagc
     4621 catccttggt tgtccggatg ggttatgagt tggtttaatt tcgtagagtg gccaatgatg
     4681 ttttttggct aatgcaacga gtttgccgcc tgcggcgata catgcaatcg ttgatttttt
     4741 tgcctgagcc tgtttagcac attcaagaat ttcttctgta ttgccagaat aacttgaaag
     4801 aatgactagt gtagattcat tgacataggc aggaagtgaa tagccatgaa taaagtctag
     4861 tggaacagtg agttgctctg tatataagtg tttaattacg tctgcaccca aacctgatcc
     4921 acccatccct gcgattacta cttgtgtaat gggagttgct ggtttaattg aaattgcacg
     4981 agtttcttgc caggcatgct ctatttgttt tccaagcgct tcaattgatc caagcatgtt
     5041 ggattgatcc agttttgcaa tactttttct gtcatttaga tcgacggaag atggtgtcat
     5101 aaagtctttc aatctacatt tatactatga tgagtggctg tatttgaggt tgtgtcgtag
     5161 atagccagtg atactactcc acttggtttg cttaatcgtg cgggaattag tgagccgggg
     5221 ttgaggatta aagttttgcc ccgaacttca ttgcgatact gatgagtatg acctgtaaaa
     5281 attgcatcat aaagcctgct tgtaattaat gcttcaagta aatcagaatc atgaccatgg
     5341 tatatagcga ttttttttcc atcaaaaagc aatggaaaca tagttttggg tgaaaacgtg
     5401 attggattgg gtagctgttg atttctctct aaaatacgtt tcagatctcc ttcgttgtta
     5461 ccaaaaacac tatagacagg tactttaaag tcactaaaag ccgaaacaaa atattccatt
     5521 gtgaatggtg ctacccaatc accacagtga cagatggcat caacactcgc tttgactaga
     5581 acctgatgtg cccaatctat gcctgggaag tgatcatgag tatcagagat tacaccaatt
     5641 ttcatactag cacctcaatt tttgatttta gggcagatgt agtgacaatg aagtcagagt
     5701 tttcggttaa cctccattca tttcctgttt tgcccaaagt tgtataacct gcttcactca
     5761 taattaatga taatcctgct atatcgtata aatctacacc aaagataatc gcggcttcta
     5821 gtttgctatg tatagcatag agtaatggtg caaccatgca accgaatttt cgaaatgatc
     5881 taaattgatg gccacaagta gctaaaaagt ggaaagaatc tgatcgagca tttgttgatt
     5941 taccgctatc gagtagtaaa gtagtagaat ctaactcagt ttcctgagaa gggttgggaa
     6001 aaggtttgtt gttaacggtg gcgccagccc ccaggctggc gctaaaaatt aaatcttgaa
     6061 ttggcgaaga gacggcggcg gcaacgatct tgccatcaat ttggagtgcc gccatcgaac
     6121 aaaatagcgg tatatgatga gaaaaattgg tagttccatc gagcggatca agaatccaag
     6181 ttggtctgga aggatctagt gatcctccat attcttcgcc gtagatggaa tggtctggat
     6241 attctgttgc gatcagcgaa cgaaaccttg tttcaatttc agtatcggtt tgtgtcacgt
     6301 atgaatggtc acttttccac tcagtactaa acttttgttg attaaaatct cgtaatgcaa
     6361 ttgctccacc ctcttgaaca agtttttccg caaaaagccg atgattttga tagttagtca
     6421 tagaagatct ttgttgaatt actggagggc atgggtattt actccttgtt tccagtcgat
     6481 ctcgggataa gcttccaaaa tcaattttaa tgttcgtttt aactcgtcgt actgagcttg
     6541 cgttttaccc tcaaacttta gagtgatata gggtccattt tgtgaagcgc gaacgattgc
     6601 catagtttct ttggtatcca ttctgatgcc atcaatatcg atatattgag cttgcggcca
     6661 ttctttttca aatgccgccc tgatttttgt tgaaacaaat tcaaacttga tcgcatctgc
     6721 caaaccaaac ttaatttctg gactactgac gtattgactg agtttgccaa ctgcttcaga
     6781 taatgtttct tttttgcgtt ccaaatatgc gagtaagcgt aagctggcaa atgcaccgtc
     6841 atcatgtcca aaaaagttat ccataaaaaa gatgtggcca gatagttctc caccaaatgg
     6901 ggctttttct tcttttacct ttgccttgat gaatgaatga cctgttaacc aaactactgg
     6961 tttgccaccg gcattttcaa ttgcttcagt tacttgacgt gagcagagtg cgttataaac
     7021 aattttttct cctgggagaa aatcaagtac gtcttgagca aacaaggcga caatggtgtc
     7081 catccacaag actttaccat tttcatccac gacggccatg cgatcgccat cggtatcata
     7141 ggcaaagcca atatcagctt tatgtttttt gacaccttct gccagacgat tgagcacttc
     7201 cacctcagtg ggatctggca ccccaagagg aaaattcccg tcaagtgtgc agttttgttc
     7261 gatcacttcg caaccggctt gccgaaaaat ttctgggtag aacatacctg aggtggtatt
     7321 gcacgcatcc accacaattt tccagcgttt ttgtaatgaa aaatgtttca aaatactttc
     7381 ggtataattg gtaaagatat cttgtttttt atcagtgccc gtaccttgac taaattctgc
     7441 tttttcgcag attaatctca attcttgaat ttcttcggtt accatggttt cggaataccc
     7501 agtgctcaac ttgagtccat taaagttgct tggattatgg gaggccgtga ccatggcacc
     7561 acctttggtt tgaaagaaat aagaggaaaa ataaacaatt tggctgagtg aatgtcctaa
     7621 atctatggtg tcaacaccac cctgatttaa tcctgtccga aaagctttgg aatattgttc
     7681 gctcgttaat cggttgtccc tgccaactgc cacctgtttg attctccatt tcgaaagata
     7741 ggtggcgtag ccaagaccaa gtgtgtaata cacatcatca tttaaatcaa cgtcaacgac
     7801 gccacgcaga tcgtaaccac gaaaaattac tgggttgatt ttcacgattt tatacatgtg
     7861 tttctcctat cttttctcgt taatttgcac taaattaagt gtcatacaaa gtatgacact
     7921 ttactttaca gctcaatttg taataggtca agtcggtttt tcagcgacag ttgtaaacca
     7981 ctggttttgc tttctacatc aatacaagtg agcttttcat gaagcattag tagctgttgg
     8041 agcgaaaaca tttttgattg tttttgaatc tttgcctgaa cgaatggggg tcctgcgatg
     8101 gtaccgccac cttttattgt aagcaagagt ctgatttgtc tcacgagcat gagaaaacaa
     8161 tactcaatgc cgtcttgggc aactgctgta ttaaataatt tcactttagt ttggattgag
     8221 gtcgaaggcg aaaatacatc gagccattga aataaggatt tactactttt gaaagttttt
     8281 tgctttgcct gactaaaagt ggctagctga tttggagtta ataatttttt ttcccagagt
     8341 attatagatt caattgtttg agatttcgtc aatgcgttaa gtaaggcagt ttgatgagta
     8401 gattttggca aagaatgcaa ttcttcaacc acggtcagca cctgctcgtt gaataagctg
     8461 acttctccca gagctgattc cagttgagcg agtgtaaggt cagtagcgag ataggtcgtt
     8521 gttcgggtaa aacgttcacg cgaagattct atatattcac gtagggcttg tcttgaagca
     8581 atgtggttgt caccgtgaaa aatatacata gctactctat taccaataat actgttgaag
     8641 agtttttttc gcactggcca aatttgggaa gtgctctgat actaatttcc aaaatgccct
     8701 actgtggttg ggatgaacca ggtgagctaa ttcgtgagtg attacataat caattactga
     8761 tggttcaaag tgaacgagac gccaattgaa agatagtgtt cctctactac tacaactccc
     8821 ccatctggtt ttttgttcct taaacccaat ggttttaaat gatacattca tgtttttagc
     8881 cagttgatga gttctcggca gaatatacgc actacacgta gacttgagga aattattcag
     8941 caatttgtgg ttcatctgaa tggttttacc tgcaatgggt gtatagagga gcttatcttg
     9001 agtaaggaca aatcctggag aatcagctgt atcgttactg tatataagat cgtatttagt
     9061 accaaagaga tatatataag tggtagtaag taccttaagt ttacccttgt gttttattga
     9121 atgcttattg atccactctt ggtgatcagc gatgaatcga ttaataataa attttggtaa
     9181 atgtggtggt gtggtaacca acacttcgcc atcgttgaca atttgtaact taattcttct
     9241 ggcttttggc tgaacttgta tagtgatgac tgcatccata ctgcagagtg tacactattt
     9301 agatttgggt gaaataattt aattcaccaa tttcttttaa ttgtttttga tactttttgg
     9361 ggttagaggc aaaaaagcta ggcaattttg gctgaaaatc tggtttcaat gactttgcta
     9421 actcaacatc ttcatgatgt agtttccccc aatacaaatc actgggatta aaatcatgtt
     9481 ggctgagcca gcgccaaacc tcaaccatgc cttggaagta aaccgcatct ttggtgaatc
     9541 ccccaggttg gctcgtatcc tcaagtcctc ttttttgacg aattgcgatg acccagcgtc
     9601 taccggggtc ttgaatgtaa cgaccgagtt tgtgccacac ttccaaaaat gaatacgatt
     9661 gagcccaact caccactagg taccgcaatg cagcaacatg agctacttga aatgattgag
     9721 gtagtaggcc atggagtgag gccaaacctt cttcagtaac taagtacgat tgaaaaccat
     9781 atttggattt tttcttaaac cagggttgtt cttcataatt taatcttcgt aatgcgtgag
     9841 tgccaatttc atgatacaga gcgctaaata aaccatcttt tctaaaatca agcggtagtc
     9901 gtatttttag aagagttgca gagattgaag ctctggagag ataggagtgt gaaaaactga
     9961 ctttaaaacg attttcgagt tgatggagtt ttaaaaatgc aaagattgaa gcttcaactt
    10021 gtgattgtgt gcagagcgtt ccctctagcg cattgaggtc agcttcgttg cgatgccaat
    10081 aggctttttc tagtatggct ttggcaagct ccaagtactt gttctcaggt ttgccaaact
    10141 gaactaattc ctcagagccc aatggttcgt cgtatgtgag tagggggtta aagttgggat
    10201 tctcaaggaa ggctaatttt tgtgcggtta cattagtggg agttatgcga ctaatcaaac
    10261 tcatgtttca atatccccaa ttaaaaattg tttgatatca tgttgtccca attctacttt
    10321 atattttgat ttatcgagtc tttgaactgc gagcatggcg gtttttacca gttggtcttt
    10381 aacttcaaac tcaacttcta ctacgggtac ttttccctcg ctcacttctt gctgttgcca
    10441 aagaagatcg tcataatcca gtaagccgag gtcaaccgcc aactttcttg atatcgcgga
    10501 gcgagcacgc aaggtgttaa ccaatgcggg cacttctaaa tcttttgtcc gatccccttt
    10561 gacaacaatc gtgggtttat ttgagataat tgtcaggcct tgctcaactc gctgttgatc
    10621 aacataaaac ttggcaaaaa gtgcttgcgc aatgcgaacc ccgtggtctg cattgcgcac
    10681 ctcaatacct tccacgcgct caattctacg cttgagtcca gctttatttg ctaattgaat
    10741 ggatagaccg gggaagccat tgagctctac caccattgga cccttggttt catcaatgaa
    10801 gagatcggcg cccataaaga taaaaccggc tgcttgggca gcactgacag ccatcaagag
    10861 ggtttctcgc cagaatggta tctggatacc acttactttt cgtttttgtt gcggaaaacg
    10921 cttaatgtat ttgcttttgc cttgaatgcc aaaagtggtt agaccactag acatatcaat
    10981 tccaagccca atagccccaa tatccagatt ggctttcccc cccgactctt cagttgggat
    11041 tcgagccatt gccataattg gaacggaatt aaaaacgatg attctgatgt ctggcgtgcc
    11101 accttttgaa agttttttta aaactggatg agcaataatc atttcttcga ccagcgctcg
    11161 atgactactg ccccaagtgc tatattcacc atccaatatg ttttgaacat gtaggtttag
    11221 atcttcgacc gtaacttctt cgttttcgtt gtcttgccaa atcgatcgct gtgggagata
    11281 tttagtaata atacgtatgc ctttgccagc gctaccactc gcgggcttga gtacaaaggg
    11341 acaaggaata ttttcccaga tcacgtggtc taaatccgtg taggattcaa acatgtggta
    11401 aagttgggcg gttgggatat tgttttccaa taaaagttgt ttggtcttca gcttagaaaa
    11461 accaaacgcc cgcgccgagc tgggattaag cttggtataa cggtagcgac cgttcatgcc
    11521 gagaatgtga gatggtttga ttggcatggt tattattctt cttcaataat tgcaccaaat
    11581 cgaagccgct cactgacgcg taatccacta aattttccaa taataatatt aacaacagct
    11641 gttaggaaaa gtagtaactc tggttcgcta agcgctattc tttggagcgt tccccattta
    11701 aataatacgc tggcaacaat agccaaaata atcgtttcga cggtaagtgc tagtgcctgg
    11761 ctttcttttg aatgggcttg cgcgtcaagg aaattttcac tcaagagcac taatattaaa
    11821 attggaaaaa tattaactga cattaattga gtaactttca aaatgggtgc caaaattaat
    11881 aagccaataa ttccaattga aatcgtccaa aggagcagcg cagttctagg caggtacggt
    11941 aagttggttt gcttcaacaa gcgactggtg ataaatgcgg tgccactgat ggccataaac
    12001 ataagtaagc caggtatcac tccagtggaa acaaaagcca ctgaaagtac cgctggaatg
    12061 tagataccaa agcctttgag accaatcacg tgtcgagaag cggcaataag cgatgcaaca
    12121 actgggaaca gaattacaac aacaataata tttgcgggaa ctccctcttt aacagtgtta
    12181 aaaattgcgt gttgtaaaaa attattccaa cttaacggtt ctggaggatt ttgatacagg
    12241 tatgcggtta actcaccgat ttgttttcca ccagtatcgg ttaagtcctg atctttcttt
    12301 tcttgaattt tttcaatgat ttcggcggag tcactggcta tttctcctgc agcggtagcg
    12361 ctggcggaat cactagcttc agtattggat aggatgattt cacctgtgac aacttcactt
    12421 ggattagttt gggcacttgc tctttcaaca aaattcacgt tgaagtgaaa aaataagctc
    12481 aagattagta atatggaaaa gacaaagtgt ttcatacgcg cagtttcttt cattactata
    12541 ggatattata acacgattga agaggaattt ccagttttga atcgaggatt gtccaataaa
    12601 gtgaattaga gaaaaattga aacaaatgat tgtgtaagac tcagcgcaaa tgagatgacg
    12661 acaagggtaa agaattcccc aagtggcacg ccaaaaagca ccattggcgc aatggtgaag
    12721 ctagggacta aatacagagc caaccaaagt aatgcaacat ttactactac tgaaaataat
    12781 cctagcgtca ctatgtttat gggcagagtt agcagtttga gtatgggttt cagaatgaga
    12841 ttaatcagag ttaaaactac tgaaaaaagt ccaagtgcaa cccaatctgt atatgaaata
    12901 ttaggcaaaa accaagctaa gataaatatg gcgagtaatg ttttaataaa aggtcgtagc
    12961 atcagtgttt acaataccac tcgattgatt tgaggtcaag ctattgtctg gtgtattatt
    13021 ttcctaataa tgagcgagtt ctcaagcgac tatgttgttt tttactgaaa cgatgagcct
    13081 catctcggat ttgttgcacg agttgtagtc ctgggtgact tgaatccaaa tttactgttg
    13141 tgtaagagat ttttagacga tttgaagacc gatcaattgt gggaaatagt aatcgatcag
    13201 ggcgtttggc aatacttatc atcggagtcg accattgcca aacgcccaaa gcagcgcgta
    13261 gttgtccttt gccgccatcg atcaccacta agtcgggtat cccccactcc gaatggtttt
    13321 gccgacgaat tatggcctcc cgaagcatct ggtaatcgtt gggggtatcc agcgtccgga
    13381 tattaaataa tcggtagtcc gcaggctcgg cctgaccatc agtaaaactt accagggcaa
    13441 ccgcggcatt tgttccgctg gtattactta catcgtaacc ctcaattttc ttcagtgaat
    13501 agtgttttgg taaaccaagg tagagttgca gcaacctgct caaatacgtt aattggttat
    13561 ctggacggct atctgcaaga ttaagtaatt gggggtctgg ttttaagtaa tatggttctt
    13621 ttgtaactgc tgtaatcgcc ttgatcatat ctcttgtttt ggctgccgcc tcgaacaggt
    13681 tgttcttgga ttgcaatttc atcgaaagct ttagttgatt gagtaaatcg cctgattttc
    13741 catggagaaa cagcgaaagt tgcttaagat ctgtttgata ctcagctagg gtaattttgc
    13801 cgcaacatac tccagagcag agattgaggt gagaatagaa gcaccccgca gtatttgtgg
    13861 gatcgtcaca ccaccgaaaa atagctcgag caattttaag gacttgttgt acttggtagc
    13921 tgctggcgaa gggaccaaaa atatttggtt ttgttgtgtt aaaaactaat tgtttttttc
    13981 taatctgaat tacttggggg actttatcag aggtgatgta aatatacaac ggacttttat
    14041 catcttttaa caagatgtta tagcttggtt gataggtgcg aatcaattcc gcctcagtca
    14101 aaatagctgc cagttcgctt ggcaattctt gatattgcac ctgctgggcc gccttaacca
    14161 tttgtaggat tctttggggt agccgtttga gttgagtata actagacaat cggcgtttga
    14221 ggttcttggc tttcccgacg tataacggtt tgccgtctat gtcggaaaac cagtaaactc
    14281 ccggtgagga gggcgcagag actaacagcg atacccacgc caaagtagaa ttcgattcta
    14341 gcgaggtagt atgggaggat gataactggt gtttcgctgt tgacatattg ggttggtcca
    14401 gactgatttg tgatggcaac tatgattgta tccttcactg gcaaaagttg gtcgctggtt
    14461 aagaatatca ggcgcttctc ggtagcgaag ggcaaaaaaa cctctgggaa attatcttgt
    14521 gaaagccatt gtaccgctac ttggtttttt tgcccacgta tcccaacgga agtattgtac
    14581 caagccttac cagtctgatt ctcaatagct acctggaaga atcctggaat aggtgcccca
    14641 tacatttgtt tattctcaaa agttgcggag taattagaag taatttcagg aaactcacta
    14701 gtaacggtaa cgatagcggt ttgttctgtt gactcgagtg gcttaaatgc tccagcggga
    14761 ggtggttgtg aactagaggt accattgtag gcaaacacta tgtgatttaa atcaaaaaag
    14821 ttgaagtaat ctacaccacc agttgtatcc tcccaagttg gatcaattgg tttccaaatt
    14881 tgatttactt catcatagta ttctggccaa gtatgtagta catctgcgtc taaactgagt
    14941 ggccgcaatt ctggttggtt agtatatgcg taccccgtag ctctgcgcgc aggtatgtca
    15001 ttgcttcgag ctatcgcaat gaataaatcg gtaaattctt gacaaaccgc atcttgagca
    15061 ttttgaaggc tgcccgcagc tccatagcgg gttctactat tggtaagtgg caattcagtg
    15121 tagtccagtt tatcgaccac tacttcgtag attgcttgag gggatttata ctgatttgcc
    15181 agcgaaacga tggcgggatc ctgagtttcc cagtattcga ggggttgagt aaatttagat
    15241 tcatttattg taagctgttt gtgtttggga tttacagaaa gttcggtgaa ggcttgcgcg
    15301 gtcacgactt tttgttcaag cggttctaag tagtattcgg ctagccaatt accatcttcg
    15361 tcaagagtta cattttctgg tttaggatca agattggtat acaacatccg ttgataggga
    15421 gtgtctggtg gcagagtgag acttgtacta actttgtagt tttgaggatt gtctaagtag
    15481 taggtaagat ccagttgata tcgctgactt tcaccaaata ctgcggtaat cccttgacct
    15541 gagtaatttt ggtatacaaa ctcactccaa ccagctaaat cacgaatggt ggttggtgaa
    15601 ggatcaattc tgtagggttg accaaattcc tttggtactc ttacaattaa ggaggatttt
    15661 gtatacgcac caacctgttg agtttttggg atagtgattt ctaatacttt tccattgagt
    15721 gtagcaaata ctgggctgga gaattgaatc gagaaatttc gctttttacc ctcgcccact
    15781 atctcatttg gaaagtcaaa ggaaattgat gttttctctg ctgttttgtc aatttgtggt
    15841 tcgagtggag atccttcatt a
//
LOCUS       DAOQZP010000025        15105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_4406_length_15105_cov_4.551296, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000025 DAOQZP010000000
VERSION     DAOQZP010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 15105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15105
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4406_length_15105_cov_4.551296"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..648)
                     /locus_tag="PKH60_02675"
     CDS             complement(70..648)
                     /locus_tag="PKH60_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015184270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNQ16985.1"
                     /translation="MMKKIERRLNAICLELVTGLLWWIVGGLPSHYTRRFFYRLAGMK
                     IGAGSTIHMMCRIYDPRNIAIGEDSIVGEMTTLDGRRQIEGSKGGITIGDHVDIASQV
                     MIWTSEHDLQDPNFKPIEEPVAIEDYVFIGPRAIILPGVTIGRGAVVAAGAVVTKSVA
                     PGTIVGGVPAKPIGTRETSKLNYKLGRARWFQ"
     gene            726..1028
                     /locus_tag="PKH60_02680"
     CDS             726..1028
                     /locus_tag="PKH60_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16986.1"
                     /translation="MHKFKDILHATTDKLIYNIAERHPKVVRMLIVGMLASLPAMAIL
                     SACDDGGTQTYDTSPGITNTIKGSQGETLQFTNPVPPPQDEYQFSNEPANGLLTGP"
     gene            complement(1250..2248)
                     /gene="ruvB"
                     /locus_tag="PKH60_02685"
     CDS             complement(1250..2248)
                     /gene="ruvB"
                     /locus_tag="PKH60_02685"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNQ16987.1"
                     /translation="MKQAKTPETTIETTPEEEKIFTSLRAGKWQEFLGQPGIKQALQI
                     AIQATHERAEALDHILLYGPPGLGKTTLAHLVAKELGVNIKMTSGTALTKTGDLAAIL
                     TSLEPHDVLFIDEIHRLPKVVEETLYPVMEDFALDIVIGKGPSARTLRLDVPRFTLIG
                     ATTRYGLLTGPFRDRFGMVHRFGHYVPEELVNIITSAAHKLETVIDSAAALVVAKRSR
                     GTPRIALKLLKRVRDFAQVEHISPIIPATVDKALSMYQIDDFGLDEHDRNLLLSILNK
                     FQGGPVGLTTLAAALSEDPGTIEEVMEPYLLQIGFIKRTPKGRVVTDAGKQHLNVS"
     gene            2348..2854
                     /locus_tag="PKH60_02690"
     CDS             2348..2854
                     /locus_tag="PKH60_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16988.1"
                     /translation="MYPLTPETSPQIPLGDATQQQPEQSPKLLPGELAGRIVGAIETM
                     ITERLNDTNASGTSEFAIKVMTTGNQGEVVLQTTATVLETELSKPLDFARNLLRGRVS
                     KDELIDRIRSGRKQFSVLFSPNWQEMALVEKMAELLRDSDEQMLLVSLDSVTSEGKTQ
                     LTRIVLGW"
     gene            complement(2867..3439)
                     /gene="ruvA"
                     /locus_tag="PKH60_02695"
     CDS             complement(2867..3439)
                     /gene="ruvA"
                     /locus_tag="PKH60_02695"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNQ16989.1"
                     /translation="MIGMLTGVVILQNQEVILDVSGVGYQVAVTSSTISKLVNGQTVT
                     LHIFTHVKEDALELFGFPELAEKKLFQLLLGVSGVGPRTALAISDLGAATITQAVQEA
                     NVARFSAIPRVGKKLAQKIIIELKSKLGSLQELELGGVTDPNQQEVVAALLSLGFEEQ
                     QIYRVFREIDASLGVAEQVKQAMKRLTSSN"
     gene            complement(3445..3927)
                     /gene="ruvC"
                     /locus_tag="PKH60_02700"
     CDS             complement(3445..3927)
                     /gene="ruvC"
                     /locus_tag="PKH60_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNQ16990.1"
                     /translation="MKAKIIIGIDPGFDRLGWAVGSVTGRKLQLLTHGLVQTSAKSSM
                     MDRYQQILVELTEILNQYQPTQAAVEELYFSTNKKTALRVSEVRGLIIGLLLNHQVAV
                     FEYHPQSIKMAITGHGKADKLAMAKQLQLELGIDTTLRDDELDAIAIALTHALTDGVS
                     "
     gene            complement(3938..5113)
                     /locus_tag="PKH60_02705"
     CDS             complement(3938..5113)
                     /locus_tag="PKH60_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNQ16991.1"
                     /translation="MKPSRLNIAIDGNEANVSKRVGSNVYAFEILKALEKLTKSQSEI
                     YHVTVLLHSQPNDDLPKSREGWSYQVFGPKQLWTQWALPRHLYAQKKTYGVFFTPSHY
                     APSICPIPYVSSVMDLAFLSHPHTFRWRDLFQLKHWTEHSAKNASRVIAISQATKTAI
                     HHYYQIKKNEIVVAYPGSPTPHTVSQSDQKQLLQSEQIQAPFFLFVGTIQPRKNIPLL
                     VAGYEQFITKFQTERLPKGINPTKIPQLVLAGKTGWMAQESLGSIRRSSVSALIKRVG
                     FSSLDLQTSLYSHALATILVSDAEGFGLPPLEAMRYGSLPIVAKAASLPEVVDKAGII
                     IPPNSPEAIADALWQCLTLSAKQKAMYRKFAREQLAFFSWEASAQIILETLQKVAKK"
     gene            complement(5110..6237)
                     /locus_tag="PKH60_02710"
     CDS             complement(5110..6237)
                     /locus_tag="PKH60_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNQ16992.1"
                     /translation="MHLGIDITSLLYDRGVSRYTRNLLLALNNLPQMQFSLYGSSLRR
                     RQDLLQQIKALHLRRETHLLQAYPVSLLSKLWQSGLNSIMPRLPGIEVFHAWDWHLPP
                     DKTLPMVITIHDLAMLRMPHIAHPNVLRAHQIAWDRISHSKTQIIAVSQTTKRDIIEL
                     LNIDPRRVQVVYEALPEEVVTAGRNLTEDTQAKIIQKFALEKPFFLFVGTQEPRKNLS
                     RIIQAWQTFAKDFDLVVAGATGWGTTNHSALRTEPIFLGPVSNLELAALYDQARLFLY
                     PSLYEGFGLPILEAFHHGTPVITSNNSGMAEVAGNAAVLVDPLEEESIRDGITTILQE
                     DRAAEKIRDQRMILRLQMFRWEDTAHQTLSVYQKTKQYWQT"
     gene            complement(6221..7750)
                     /locus_tag="PKH60_02715"
     CDS             complement(6221..7750)
                     /locus_tag="PKH60_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16993.1"
                     /translation="MEKHWYWLVGLMLLLALLLRLPLLSGSFWLDEAAQALESARPLS
                     QQFNIRDDFQPPLLHLIVHFAQYGSRAEWWQRLFGALIPGIFSILGTMLIARKILPKN
                     FSSTGSLLVGILLATNSFHLFFSQELRPYALPMLFAVWSWWVLLQAKHSPKYIISWAI
                     LSLLGLYGSYLYPFLLLSQCIYLGGRFWKTKLIQMWLAGLLAIILGFIPWLPSFVGQL
                     QAGQLLRLQLPQWETAVSTSQLKVLPLVLGKFIYGVLDISFNQYFLGSSALIFLSFSI
                     IAIGLLKQQLSKELSSKDKKALILLLIWLLVPLLSAWLVSFFVPVIQPKRVIFLLPAF
                     YLLVVWVFATSQSMSFWFRRAGCALVGIILLINLFSVHQYYTDQRLQRENWRDVLTAV
                     SRKHPQAFTLSSFPAPFAPIEWYRPAFINSPMAQDLASGTLHIDSQATADQIIDDITA
                     LKLTSTMIYFEYLTDLTDPNQYLRTAIENSGYQLQDLLDYPGIGFVRIYQYTPSYALR
                     D"
     gene            7789..8778
                     /locus_tag="PKH60_02720"
     CDS             7789..8778
                     /locus_tag="PKH60_02720"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNQ16994.1"
                     /translation="MLDLQNKNILVTGAAGFIGSHLVDSLLEKGAKVTGVDNFLTGNI
                     DNLTQAKRSANFTFIQADAIEPPQVFLPADYHPDIVFHLASPASPPLYQKNPVETYLV
                     NTMGTHQLLSWLLEHSDTESTSRFVFTSTSEVYGDPLEHPQKETYWGNVNPNGPRSCY
                     DESKRMGETICGVFHRDFGLDVRIARIFNTYGPRMSESDGRIIPNLVSQAKAGEPFTI
                     YGDGSQTRAYCYVADLVDGLLRLVTVEEASGKTVNLGNPEEMTILETAKQVHATFAST
                     NQPFATVLKPLPVDDPVRRCPDISLAKSLLGWQPTTPFAQGLEMLFDDYHRTI"
     gene            8756..10960
                     /locus_tag="PKH60_02725"
     CDS             8756..10960
                     /locus_tag="PKH60_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16995.1"
                     /translation="MITTEQFKSFDDALHFSPLLKEIQEVTERFYSNNEYFTELLTPT
                     PINMGFGWEEGVEQPKRGTETIIKEMEALLPIAEFDVAQFSRTKEQVGKSIKFKIGIV
                     YAADPHTYMAPPAQQVVDSYRTAFWEYLHFLPISESQLRETIVEFPNGLPKGRLATYL
                     PLHSIVWCFMEVDGKIQPIDAMTLMTGGGFLKGLFHSEDPTEQQFLEKEKHRLQLSLL
                     TTMSEYSHVAAVALYHLYEEYFMSVFIDTINRHRAEFHISEQWSEYELRKNFSLFRKG
                     IRRHMNIGYDPAEPEVGEWFSLIDGARTDIDAGRGPASQPSAHIFLREISFKKWIRLF
                     TKFLDEQSSEQQFGSEEYQQLDQLVRLALLEADAWSPDGEVGRLSETHGVEDLREERH
                     LFGHIHRSKFLAENPARFIKALDPYANWEGPAILDLLYEPLIKGLSEYQIYDVENVEE
                     QADAMVPYHRNISAITMKFPIDKQPGEILTSLGGILSEVTRAKIAFFRPENDLGEPYV
                     FLSAEKIAVWHGKLSNYRQTRKMVDPLKKSEYQLNAPIQDVMAILEDWSEVDQINLFK
                     QFLAQILLYVWQDIQESDFNWESMGNIDELLDRLFSADLEMSHPKITQIWVLMRAIFS
                     YRGGILQLSASKAFSRVLHHTASAGKLDEQKLANYIVVKTLENEQLGIRPEGQRPLNS
                     TRLPGFLASFDYIHEQSNYLQVTLSPIISERGSMEQIKGLVLERFFDKPLRM"
     gene            11069..12007
                     /locus_tag="PKH60_02730"
     CDS             11069..12007
                     /locus_tag="PKH60_02730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ16996.1"
                     /translation="MPTKSNTATVSVIIPTLLAQPEWLRRTLTSLSKQTYSGSFSVVV
                     VVNAAQSALDAFLSTFNVTKTNVKLTWIVQGKNTGFTNAVNRGITATKSKLIALVNDD
                     VWLAENWLAEIIATWQQTKADMIASTIYTPKKHPFAWPLKPTQWQLDSCGFDFAWRGK
                     AWPLLPNQKNELTSPLSPCWDNWLQHVDLLGKIAFADTLQPFGADGAAALYTRKLFNR
                     AGLFKQSFFAYLEDVELALRARRSGFICRWSPKALAYHGKHATSTQMGNFKAKQDVKN
                     WWRMLLSFPFDAWWRFGVSILLERLRNVSGLLKQLK"
     gene            12041..13003
                     /locus_tag="PKH60_02735"
     CDS             12041..13003
                     /locus_tag="PKH60_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16997.1"
                     /translation="MSEQLIFFVSGLVLVLVSARYLLLTVQHFSARLQLSPLIVGTII
                     MAIGANLPELSLTITSVYNQDPGLALGNSVGSVLVNLGLIFGISIIFGKIRIGTHKTQ
                     IEAIIALLVTSIFVFVTISSFTGMQQSLVLLTTCAVSFGILIFLSYMGRKHEDISVIH
                     KLLYRLRNQHRWSWKKMTLVGIISIVGLTGGSYLVVSAVESFSVLIGISTTILGLTLT
                     SLTTSLPELAVTILGAKAKEEKTIVGLLIGSSLLNLALFPAIVGWSGSQIQVAPIELI
                     WLLLLMVAVVTIIILHKGKVVTAKYGLILITIWLAFMASNYIFR"
     gene            13032..14189
                     /locus_tag="PKH60_02740"
     CDS             13032..14189
                     /locus_tag="PKH60_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16998.1"
                     /translation="MRFRKTETKQPPVERSRLHLLAAILTGSSVVSLGLTSVVLRVLS
                     QPDRSKYLLKVSLAEYIRTLHDFVRPPIVFEPGDWVLTEDTETQQNEHQSKEIDANRL
                     DLKALVLDGVPEFTGDYRFIHYSADGSPRNHTLRLTDHGTIGKDENLQNTVLGQDKGK
                     LENPTSLAAISIDSLPTLVTASLAKTLTEPRQMVPKDHFASEQLRHVEQTVLNIFANG
                     WLPELPATITFLATDIGLPMGEIKEQLYTMQKNLATSIEGFQDSVDILSYVYLIKTAM
                     ALPIAVGQSYIASQLQGNEKPIVTRRRFIQLVTGGAIVGGHLAMGNHSGQIAIDVNAI
                     RKEIDELAWMFNLPFQVKDRMPANFIGRVHSFLDKNEQQIPAIAYFLFVRE"
     gene            14214..14567
                     /locus_tag="PKH60_02745"
     CDS             14214..14567
                     /locus_tag="PKH60_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ16999.1"
                     /translation="MLQSGNYTSDMQPNSMSLWGNVHDTKIGLYSLTKSELLTFITQA
                     ITRFPTLFKLCFGQQPTPEEALNNRAYSGAHELYTASAYRADAHGVNKEWNISAIEVW
                     QFPELKAIVENGLVS"
     gene            14590..>15105
                     /locus_tag="PKH60_02750"
     CDS             14590..>15105
                     /locus_tag="PKH60_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17000.1"
                     /translation="MNDDQAQTSNLTLDAPPEAITELDPLIDRGEATEVLPEPLTEPE
                     MAVENAKKIEAMYKASNNIVHLMVELKVVSQDTVDNFQLDDSSPKIQTLRAEYAKVTQ
                     EIADKQDSDADVQAQSALQAELVISYQYAVAALVQKEFKPYMYKAILDASRKLRVLDD
                     ASATINKAEFDR"
BASE COUNT         4376 a         3099 c         3367 g         4263 t
ORIGIN      
        1 attgtgtata aaaagcagga agttacgaaa agagaaagtt ggtagatcag cttctttctg
       61 tgttaggatt tattgaaacc agcgggctcg tccaagtttg tagtttaatt tagaagtctc
      121 acgagtgccg atgggtttgg cggggacacc gccgacgatg gtgccgggcg cgacggattt
      181 agtgacgacg gcgccggcgg ccacgacggc gccacgacca atggtcactc caggaagaat
      241 tattgcccgt gggccaataa acacgtagtc ttcgattgcc acaggttctt caattggttt
      301 gaagtttgga tcttgcaagt catgttcact cgtccaaatc atcacttggg aagcgatatc
      361 aacgtggtcg ccaatagtga taccgccctt tgatccctca atttgtcggc gaccatctag
      421 cgtggtcatt tcaccaacaa ttgaatcttc gccgatagcg atgttgcggg gatcatagat
      481 tcgacacatc atgtgaattg tggatccagc accaattttc atgccggcta atcgatagaa
      541 aaatctccgt gtatagtgtg acggcaatcc tcccacaatc caccagagta atccagtgac
      601 tagctcgagg caaatagcgt tcagacgtcg ttcaattttt ttcatcataa tttcattgta
      661 tcattgacac aaatcctgtt ataggatact atactgcata gtaaagatac aaagaaatca
      721 cttgtatgca caaatttaaa gatattttgc atgctacaac agacaagctc atttataata
      781 ttgctgaacg ccacccaaaa gtcgttcgta tgctgatcgt gggtatgctc gctagtcttc
      841 ctgccatggc gatactttca gcatgtgacg atggcggtac acaaacttac gatacgagtc
      901 caggcataac gaatacaatt aaaggctctc agggtgagac gcttcagttt acgaatccag
      961 tcccaccgcc tcaagatgaa tatcaatttt caaatgaacc ggctaatggc ttgcttacgg
     1021 gtccataatt ttagaaaaaa ttgattctgc atacaatccc ctcttaactg gcaggtacca
     1081 ttcggaggag atttttagtt tacaaatcct taaaggtaaa aaaacatacc cacattagaa
     1141 atagtagtgg gaaatcacac atcactttca aaatcatggt ttacactaaa gcatgactcc
     1201 agacaaggtt atgctttaca ctaaactata caattaaaat agtgtgtggt taacttacat
     1261 tgagatgctg tttgccggcg tcagttacaa cccgaccttt tggcgtgcgt ttgatgaagc
     1321 caatttgtaa caaatatggc tccatgactt cctcaatcgt gccaggatct tcgctgagcg
     1381 cggctgcgag agtggttaag ccaacgggtc caccctgaaa tttatttaag atagaaagca
     1441 ataaattgcg atcatgttcg tccaaaccaa aatcgtcgat ttggtacata ctcagagctt
     1501 tgtcaactgt ggcaggaata attggagaaa tatgttccac ctgagcaaag tcgcgcacgc
     1561 gcttgagcaa tttgagcgcg atgcgaggag tgccgcgcga gcgcttcgcc acaaccaagg
     1621 cggcggcgga atctataaca gtttccaatt tgtgcgccgc cgaagtaata atattcacca
     1681 attcttctgg tacataatgg ccaaaccgat gcaccatgcc aaatcgatct ctaaacggcc
     1741 cggttagcaa accatagcga gtagtggcgc caatcaaagt aaacctcggt acgtccaggc
     1801 gtaacgtccg tgctgaaggt cctttgccaa tcacaatatc tagggcaaaa tcttccatca
     1861 ccgggtatag cgtttcttca actacttttg gtaatcgatg aatttcatca ataaataata
     1921 cgtcgtgggg ttccaaactc gtcaaaatcg ctgccaagtc accggttttt gtgagtgcag
     1981 tgccactcgt catcttgata ttcacgccta attctttggc caccagatgt gccagcgtgg
     2041 ttttacccaa gcccggagga ccatagagca aaatatgatc gagtgcctct gcccgttcgt
     2101 gtgtagcttg aatagcgatt tgtaatgcct gtttaattcc tggctggcca agaaattcct
     2161 gccacttccc cgctctgagc gaagtaaaga tcttttcctc ttctggagtt gtttcgatag
     2221 tcgtttcggg agtttttgct tgtttcatgt attctttggt tattgtacac gaaatacgac
     2281 ctcccgtaag taatttgatt acttgcgata taatataaac atatagaaat agaacataag
     2341 gagttgtatg tatccactca caccagaaac aagtcctcaa ataccactcg gagatgccac
     2401 tcaacaacaa ccagagcaat cacccaagct gctacctggc gaactagctg ggagaatcgt
     2461 tggggcgatt gaaacgatga ttacagaaag actcaatgat actaacgcaa gtggaacaag
     2521 tgagtttgct attaaagtga tgaccactgg caatcaggga gaggttgttc ttcaaacaac
     2581 tgcaactgtg cttgaaacag aactcagtaa accactggat tttgctcgaa atctcctacg
     2641 aggaagagta tctaaagatg aactaatcga taggatccgc tcaggtagga aacaattcag
     2701 tgtactgttt tcgcctaatt ggcaagagat ggctctagtc gaaaaaatgg cagaactttt
     2761 gcgggattct gatgagcaga tgctccttgt ttcacttgat tcagtgacaa gcgaaggcaa
     2821 aactcaatta actcgaattg ttttggggtg gtaaacagcc tgctgcttaa ttagatgaag
     2881 ttagtcgctt catcgcctgc ttaacctgtt ctgcgacacc aagtgaagca tcgatttctc
     2941 tgaagactcg ataaatttgc tgttcttcaa atcctaagct caatagggcg gcgacaactt
     3001 cttgctggtt gggatctgtc actccaccaa gttcaagttc ttgtagactg cccaacttgc
     3061 tttttaattc aataatgatt ttttgggcta gtttttttcc aactctagga atcgcggaaa
     3121 atcgagcaac attggcttct tgcactgctt gggtaattgt tgccgctcct aagtcagaaa
     3181 tcgcaagtgc agttcgtgga ccgactccac tcaccccaag taacaattga aaaagttttt
     3241 tttctgctaa ttctggaaaa ccaaataatt ctagagcgtc ttctttgaca tgggtaaaaa
     3301 tatggagcgt tactgtttgt ccattaacta actttgagat tgtggaggat gtgactgcca
     3361 cttggtaccc cactccgctt acatctaaaa tcacctcttg gttttgaaga attacaacgc
     3421 cagtgagcat gccaatcata gcttttatga tacaccatct gtgagagcgt gagtgagggc
     3481 gatggcgatg gcgtctagtt cgtcgtcacg caacgttgta tctattccaa gttcaagctg
     3541 tagttgtttg gccatcgcca atttatctgc tttaccatgg ccagtaattg ccatcttgat
     3601 tgattgggga tgatactcaa atacagcgac ttgatgattg agcaatagtc caataatgag
     3661 tcctcttact tccgacactc tgagtgctgt ttttttattg gtagaaaaat agagttcctc
     3721 tactgccgct tgagttggtt gatattggtt gagaatttca gtcagttcga caagaatctg
     3781 ttggtagcga tccatcattg aggatttggc agaggtctgt accaagccat gagttaacaa
     3841 ttgcagcttt ctaccagtga cagaaccaac agcccaccca agtcgatcaa atccaggatc
     3901 aataccaatg attatttttg ctttcaaaga aaatccttta tttttttgcc actttttgta
     3961 acgtttcgag aatgatctgg gcagatgctt cccaactaaa aaacgccaat tgttcgcgag
     4021 caaatttgcg gtacatggcc ttttgttttg ctgaaagggt gagacattgc catagcgcgt
     4081 ctgcaatagc ttctggtgaa tttggtggaa tgattattcc tgccttgtca actacttctg
     4141 gtagactggc ggctttggcc acaattggaa gagaaccata gcgcatggct tctagtggtg
     4201 gtaacccaaa cccttcggca tcactcacta aaatagtggc tagcgcatgt gaatacagtg
     4261 atgtttgtaa gtcaagactg ctaaaaccta ctcgtttgat tagggctgaa actgaagagc
     4321 gacgaattga ccccaagctt tcttgagcca tccagccggt tttgcccgcc aagactaact
     4381 gagggatttt tgttggattt atgccttttg gcaggcgctc ggtttgaaat ttcgttataa
     4441 attgctcgta accagcgacg agtaagggaa tattttttct gggctgaata gtaccaacaa
     4501 ataagaagaa aggagcttgt atctgctcag attgcaacag ctgtttttgg tctgactgac
     4561 taactgtatg tggggtgggt gaaccaggat atgcaacaac aatttcgttt ttcttaatct
     4621 ggtaatagtg atggatggct gttttagttg cttggctaat tgctataact ctactggcat
     4681 tttttgcact atgctctgtc caatgtttga gttggaatag atctcgccag cgaaaggtgt
     4741 ggggatgtga aagaaatgct aaatccatta cagatgaaac atagggaatt ggacaaatac
     4801 ttggtgcgta atgactgggg gtaaaaaata caccataggt ttttttttga gcatagagat
     4861 gtctcggcaa agcccactga gtccagagtt gtttggggcc aaaaacctga tagctccaac
     4921 cttcgcgtga ttttggtaag tcatcatttg gctgtgagtg aagtagtaca gtaacgtgat
     4981 atatttcgct ctgagatttt gtgagctttt ccaaagcttt taaaatttca aaggcataca
     5041 cattgctgcc tacccgtttt gagacatttg cctcattgcc atcaatggcg atgttgagtc
     5101 ttgatggttt catgtttgcc agtattgttt agttttttga tacacactca gtgtttggtg
     5161 cgcagtatct tcccagcgaa acatttgtag tctgagaatc attcgttggt cacgaatctt
     5221 ctctgcagct cgatcttctt gtaagatggt agtaatgcca tcacgaatgc tttcttcttc
     5281 aagtggatca actaagacag cggcattacc tgctacttca gccataccgg aattgttgct
     5341 tgtgattacc ggtgtaccgt ggtgaaaggc ttcaagaatt ggtaggccaa aaccctcgta
     5401 cagagaggga tagagaaaga gacgagcttg atcatagagt gccgcgagtt caagattact
     5461 tactgggcca agaaaaattg gttcggtcct cagtgcagaa tgattagttg taccccaccc
     5521 agtggctccc gcaacgacta aatcgaaatc tttagcaaag gtttgccaag cctgaatgat
     5581 tcgacttaga ttctttcttg gctcttgagt accgacaaac agaaaaaaag gtttttcgag
     5641 tgcaaatttt tgaataatct tcgcctgtgt atcttctgtc aaatttctac ctgcggtaac
     5701 tacttcctcg ggaagagcct catagacaac ttgaacgcga cgtggatcga tgtttaaaag
     5761 ctcgatgatg tctcgttttg tcgtttgact gacagcgatt atttgcgttt ttgaatgtga
     5821 aattcgatcc caggcaatct ggtgtgctcg taatacattg gggtgagcaa tatgtggcat
     5881 tcgtaacatg gccaaatcat gtatggtgat gaccatgggc aacgttttat ctggaggcaa
     5941 atgccaatcc caagcgtgaa atacttcaat gcctggtaaa cgaggcatga tggagtttaa
     6001 gcctgattgc cagagtttgg agagcagtga gaccggatag gcctggagta aatgtgtttc
     6061 ccgtcgtaaa tgcaaagcct tgatttgttg gagtaagtct tgtcttcgac gcaacgaact
     6121 gccatacaac gaaaattgca tttgcggcag attgtttagt gctaacagca aatttcgagt
     6181 ataccgtgaa acgcctcgat catacaataa gctggttata tcaatcccta agtgcataac
     6241 ttggtgtgta ctgatagata cggacaaaac caatgccagg atagtctagc aaatcttgga
     6301 gctggtagcc agaattctcg atcgcagttc taagatactg attgggatcg gtcaaatcgg
     6361 ttaaatattc aaagtaaatc atagtgctgg tcaatttaag tgcagtaata tcatcaataa
     6421 tttggtcggc agttgcttgt gagtcaatat gcagtgtacc actagcaaga tcttgagcca
     6481 tcggtgaatt aatgaaggca ggtctatacc attcaattgg tgcaaatggg gcgggaaaac
     6541 tgctcagcgt aaaagcttgc ggatgcttac gactgactgc tgttagtaca tcgcgccagt
     6601 tttcccgctg cagacgttga tcggtgtagt attgatggac cgaaaacaaa ttgattagca
     6661 gaatgatgcc cactaatgcg cagccagctc gacgaaacca aaaactcatg gattgagacg
     6721 ttgcgaatac ccaaaccacc aacaagtaaa aggccgggag taaaaatatg actcgttttg
     6781 gttggatgac tggaacaaaa aaagagacca accaggcgct caataatggg acaagaagcc
     6841 aaatcagaag caaaataaga gcctttttgt cttttgaaga gagttctttt gatagctgct
     6901 gtttgagtaa tccaatggct atgatactaa aggacaagaa aatcaaagca gaactgccca
     6961 gaaaatattg attgaaagag atgtcgagaa ctccatatat aaatttcccc agcactaaag
     7021 gtagtacctt caattgacta gtgctcaccg cagtttccca ctggggtagt tgtaatcgaa
     7081 ggagctggcc ggcttggagt tgaccaacaa atgatggtag ccacggaata aatcccaaaa
     7141 tgatggccag caagccggcc agccacattt gtatcaattt agttttccaa aatcttcctc
     7201 caagatagat gcactgggag agtaacaaaa atggatataa atatgaacca tacaaaccca
     7261 gtaacgaaag tatcgcccaa cttataatgt atttgggtga atgctttgct tgcagtagta
     7321 cccaccaact ccaaacagca aaaagcatgg ggagggcgta tggtcgaagt tcttgagaaa
     7381 aaaagaggtg aaatgaattg gtggcaagca agatgcccac tagaagactg ccagttgagg
     7441 agaaattctt tggtaggatt ttccgcgcta tcagcattgt acctaggatt gagaaaatcc
     7501 cggggattag agcaccaaac aatcgttgcc accactctgc cctacttcca tattgtgcga
     7561 agtgaacaat caaatgtaag aggggtggct gaaaatcatc gcgaatgttg aattgctgac
     7621 tcaggggacg cgcgctttct aacgcttgag cagcctcgtc gagccaaaac gagccactca
     7681 gtagtggtag tctgagtaat agggcgagca ataacatcaa tcccactaac cagtaccaat
     7741 gtttttccat acaagtatta tgccatcatt tgctacaatg atgcatcgat gttggattta
     7801 caaaataaaa acattctagt tactggcgca gcaggtttta ttggctctca cttagttgat
     7861 agtttgctag aaaaaggtgc taaagtgacg ggcgttgaca attttttaac tggcaatatc
     7921 gacaatctta cgcaggcaaa acgctctgcc aattttacat ttattcaggc cgatgcgatt
     7981 gagccaccgc aagttttttt gcccgctgat tatcatccag atattgtctt tcatttggct
     8041 tctccagcta gtcctccact ctaccaaaaa aatcctgttg aaacctattt ggtcaacacc
     8101 atgggcaccc accaattgtt gagctggttg cttgaacact ctgacacgga atcaacatct
     8161 cgatttgtct tcacgagtac cagcgaagtc tatggcgatc cgcttgagca tccgcaaaaa
     8221 gaaacctatt ggggcaatgt caatccaaat ggtccgcgtt cttgttatga tgaaagtaag
     8281 cgcatgggcg aaactatttg cggtgtgttt catcgtgatt ttgggcttga tgtgcgaatt
     8341 gcgcggattt ttaataccta tggtccgcgt atgagtgaat ctgatggtag gattattccc
     8401 aacttggtga gccaagcgaa agctggcgag ccgttcacaa tttatggcga tggctcgcaa
     8461 acgcgagcct actgttatgt tgctgattta gtcgatgggc tattgcggct cgtcaccgtt
     8521 gaggaagcta gtggtaaaac cgtaaatttg ggcaatcctg aagaaatgac gatcttggag
     8581 actgccaagc aagttcatgc aacattcgca tcaactaatc aaccatttgc cactgtactc
     8641 aagccattac cagtggatga tcccgtcagg agatgcccag acatttcttt ggcaaaatca
     8701 ttacttggtt ggcaacctac cactcccttt gcccaaggat tagaaatgct gtttgatgat
     8761 taccacagaa caatttaagt cgtttgacga cgcgcttcat ttttcaccgt tactgaaaga
     8821 aattcaggaa gttactgaac gattttattc aaataatgaa tattttactg aacttttaac
     8881 ccccactcca atcaatatgg gttttggttg ggaggaagga gtggaacagc caaaaagagg
     8941 aacggagaca atcattaaag aaatggaagc gcttctacca atagcggaat ttgatgtagc
     9001 gcagttttct agaacaaaag aacaagttgg aaaatcaatt aaattcaaaa ttggaattgt
     9061 atatgcagct gatccacata catacatggc tcctccagcg caacaagtgg tggattcata
     9121 ccgaacagca ttttgggagt acttacattt tttaccaatt tcagaatcac aattaagaga
     9181 gacaattgta gaatttccca acggtttacc aaaaggtaga ttggcaactt atttgccact
     9241 acactcgatt gtttggtgtt ttatggaagt tgacggcaaa atacaaccca tcgacgcaat
     9301 gacgttgatg actggcggcg gttttctcaa aggactgttt cattcagaag atccaactga
     9361 gcagcaattt ttagagaaag aaaaacatcg tttgcaatta agtttattga caaccatgag
     9421 tgaatattcg catgtggcgg cagttgcgct ttaccatctc tacgaagaat attttatgtc
     9481 tgtttttatt gacacgatta accgtcaccg agctgaattt catatttctg aacagtggag
     9541 tgaatatgaa ttacgcaaga attttagtct atttcgcaag ggcatacgaa gacacatgaa
     9601 tattggctat gatcccgccg aacctgaggt gggagaatgg ttttcattga tcgatggtgc
     9661 tagaaccgat attgatgcag gcagaggtcc tgcttctcaa ccgagtgccc atatcttttt
     9721 acgagaaatt tcctttaaaa aatggatacg attgtttact aaatttttgg atgaacaatc
     9781 atccgaacaa caatttggca gcgaagaata tcaacaacta gaccagttag tacgcctggc
     9841 gttacttgag gctgatgctt ggagtcccga tggagaagtt ggtagactca gtgaaacaca
     9901 tggtgtagaa gatttgcgtg aagaacgaca tttatttggg catattcacc gatcaaaatt
     9961 tttagctgaa aaccctgctc gattcatcaa ggctctagat ccatacgcga attgggaggg
    10021 accggcaatt ctggatcttc tgtatgagcc attaataaaa ggtttaagcg aatatcaaat
    10081 ctatgatgtt gaaaatgttg aggaacaagc agatgcgatg gtgccgtatc accgtaatat
    10141 tagtgcaatt acgatgaaat ttccgattga taaacaacct ggtgagattt taaccagtct
    10201 gggaggaatt ttaagtgaag ttactagggc aaaaatcgcc tttttcagac cagagaatga
    10261 tttgggtgag ccatatgtat ttttatctgc agaaaaaatt gctgtttggc atggtaaatt
    10321 gtccaattat cggcaaacgc gtaaaatggt tgatcccttg aagaaatcgg agtatcaact
    10381 gaacgcccct attcaagatg taatggcaat tcttgaagac tggtctgaag ttgatcagat
    10441 taatttgttt aaacaatttt tagctcaaat attactgtat gtttggcaag atattcagga
    10501 aagtgatttt aactgggaaa gtatgggaaa tattgatgaa ctattggaca gattgttttc
    10561 tgctgattta gagatgagtc atcccaaaat tactcaaatt tgggtgctga tgcgagccat
    10621 ctttagttat cgaggtggaa ttttacaatt gagtgcctcg aaagcatttt cacgagtcct
    10681 gcatcacaca gcttcggcag gcaagctcga tgagcaaaag ttggccaact atatagttgt
    10741 aaaaacctta gaaaatgaac aattaggcat ccgtccagag ggtcagagac ccttgaatag
    10801 tactcggctt ccagggtttc tcgcctcatt tgactatatc catgagcaat caaattactt
    10861 gcaagtaacg ctatctccca tcatcagtga acgaggtagc atggaacaaa ttaaaggact
    10921 agttttggaa cgattttttg acaaacctct tagaatgtaa atagtgcaag ttgcccaacg
    10981 gttgattttt tgtcatagta agaaaagtta gtcatcaaaa accgcagtac tattttgatc
    11041 tattttctga taggataact gattcggtat gcctacaaag tcaaacacag caacagtaag
    11101 tgtaatcatt ccaacattgt tggcgcagcc agaatggctg cgccgaaccc taacctcact
    11161 gagtaaacaa acgtactcag gatcattttc tgtggttgtc gttgtgaatg cagcgcaatc
    11221 tgctctagat gcttttcttt caacattcaa tgtaaccaaa actaatgtta agctcacttg
    11281 gattgttcaa ggtaaaaata ctggattcac gaatgcggtc aaccgtggca tcactgccac
    11341 aaaaagcaag ctaatcgcac tagtgaatga cgacgtctgg ttagccgaaa attggttggc
    11401 agaaattatt gctacttggc aacaaaccaa ggcagatatg atcgccagca ctatttacac
    11461 ccccaaaaaa catccatttg cttggccgct caaaccaact cagtggcagc tggattcttg
    11521 tggttttgat tttgcttggc gcggtaaagc atggccattg cttcccaatc aaaaaaatga
    11581 gcttacctct cccctcagtc cttgttggga taattggctg cagcatgtgg acttgttggg
    11641 caagattgcc tttgcggata cgctccaacc atttggagct gatggcgctg cagccctcta
    11701 tacccgcaag ctatttaatc gagcaggatt gtttaaacaa tcattttttg catacttaga
    11761 agacgtggaa ttagctctcc gagccagaag atcaggtttt atttgcagat ggtcacccaa
    11821 agccttggca tatcatggca agcatgccac ttccactcaa atgggcaatt ttaaagccaa
    11881 gcaggatgtt aaaaactggt ggcgcatgct cctttcattt cccttcgatg catggtggcg
    11941 atttggagtg agtattttgc ttgaaagatt gagaaacgtt tccggcttgc tcaaacagct
    12001 taagtagaca acttcttcaa taaaatgcta ctatggtttg gtgagtgaac aactaatttt
    12061 ctttgtgagc ggtcttgttc tagtgctggt ttcagctcgg tatttattat taacagttca
    12121 gcacttttct gccagactcc agctctcccc gctgatcgtt ggaacgatca tcatggcaat
    12181 tggtgcaaat cttcctgagc tctcactcac tatcacatct gtatataacc aggatcctgg
    12241 actagcactg ggcaactctg tgggtagtgt gctcgttaat ctggggctga tatttggtat
    12301 ttcgatcatc ttcggcaaga ttagaattgg tacacataaa acgcaaatcg aagccatcat
    12361 tgccctactc gtcacgagca tatttgtttt tgtaaccatc agtagtttta ctggaatgca
    12421 acaatctttg gtgttgctga cgacctgcgc tgtaagcttt ggcatcttaa tcttcttgtc
    12481 ttacatggga agaaagcatg aagatatcag tgtgatccac aagttactgt accggctgcg
    12541 aaatcaacat cggtggtcat ggaaaaaaat gactttagtg ggaatcatct ccattgtggg
    12601 tttgactggc ggcagttact tggttgtttc agcggtggaa agttttagtg ttttaattgg
    12661 tatttccaca acgattttag gacttaccct tacttcacta accacctcat tgccagaact
    12721 ggctgttact attttaggag caaaagccaa agaagaaaaa acaatcgtag gactgttaat
    12781 cggcagtagt ctgctcaatc tagcgctctt tcctgccatc gttggctgga gtggaagtca
    12841 aattcaagtt gccccaatag agctgatttg gttgctttta ctcatggtag ctgttgtcac
    12901 catcattatt ttacataaag ggaaagtcgt gacggctaaa tatggtttga ttttgattac
    12961 gatttggcta gcctttatgg cgagcaatta tatattcaga taaatcttct caactgatat
    13021 aataatctct tatgagattt agaaagactg aaactaagca gccaccggta gaacgatcgc
    13081 gcctgcattt gcttgcagct attttgactg gtagttctgt ggtgagtttg ggtttgacga
    13141 gtgttgtttt gcgtgtgctt tctcaaccag accgctcaaa atatcttctt aaagtatcgt
    13201 tggcagaata tattcggaca ttacatgact ttgtgagacc accgattgtt tttgaaccag
    13261 gagattgggt tctgactgag gatacagaaa cacagcaaaa tgagcatcaa tccaaggaaa
    13321 tagacgcaaa caggcttgat ttgaaagcat tggtgctcga tggtgtacca gagtttactg
    13381 gcgattaccg atttatacac tattccgctg atggaagtcc tagaaaccac acattgcgac
    13441 tgactgatca tggtactatc ggcaaggacg aaaatttgca aaacacagtg ctgggtcaag
    13501 acaaagggaa actggaaaat ccaaccagct tggcagctat ctcaattgac agcctaccga
    13561 ctctcgtcac agcaagtttg gcaaaaactc ttaccgagcc aagacaaatg gtgcccaaag
    13621 accattttgc ttcggaacaa ttgcgacatg ttgaacagac ggtgttgaat atcttcgcca
    13681 acggctggtt gcctgaacta ccagcgacaa ttactttttt ggctacagat attggtttgc
    13741 caatgggaga aatcaaggaa caactctata caatgcaaaa aaacttagct acttcaattg
    13801 aaggatttca ggatagtgtt gatatactgt cgtacgtata cctgattaaa acagccatgg
    13861 cacttcccat tgccgttggt cagagctaca tagcctcgca actccaagga aatgaaaaac
    13921 cgattgttac cagaagacgg tttatccagt tggttaccgg aggggctatc gttggcggtc
    13981 atttggccat gggtaatcat tctgggcaaa ttgccattga tgtgaatgcc atcaggaaag
    14041 aaatcgatga actagcttgg atgtttaatc tgccattcca ggtgaaagac agaatgccgg
    14101 ccaattttat tggccgcgta catagtttct tggataaaaa tgaacagcaa attcccgcca
    14161 ttgcttattt cctatttgta cgagaatagg taacggcata caaggaaata gcgatgttgc
    14221 aatctggtaa ttacaccagc gacatgcaac ctaacagcat gtcgctatgg gggaacgttc
    14281 atgatacaaa aattggtttg tactcactca ccaagagcga actgctgaca tttatcactc
    14341 aagcaattac tcgcttcccc actctgttca aactgtgttt tgggcaacaa cccactccag
    14401 aggaagcact gaataatcgt gcctattcgg gagctcatga gctctacacc gccagtgcat
    14461 accgggctga tgctcatggc gtcaacaaag aatggaatat ttctgccatt gaagtctggc
    14521 aatttcccga actcaaagcg attgtggaaa atggtctggt aagctgagtt tatgataaac
    14581 tgaagatata tgaatgatga tcaagcacaa acttcaaact taactctaga tgcaccacca
    14641 gaggcaatta ctgagttgga tcccctcatt gatagaggcg aagctaccga agttctccca
    14701 gaacccctaa ctgagccaga gatggcggtg gagaatgcaa aaaaaattga agcaatgtac
    14761 aaagcctcaa ataacattgt acatttgatg gttgagctaa aagttgtgag tcaggacaca
    14821 gtggataatt ttcagctaga cgattcttca cccaaaattc aaacccttag agctgagtat
    14881 gccaaggtaa ctcaggaaat agcagacaag caggatagtg atgccgatgt gcaagctcaa
    14941 agtgcattgc aagctgagtt ggttatttcc tatcaatacg cagtggcggc tttggtacaa
    15001 aaagaattta aaccatatat gtataaggca attcttgatg catcaaggaa gttgagagta
    15061 cttgatgacg caagtgcgac tataaacaaa gctgagtttg atcga
//
LOCUS       DAOQZP010000026        14838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_4503_length_14838_cov_5.521951, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000026 DAOQZP010000000
VERSION     DAOQZP010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 14838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14838
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4503_length_14838_cov_5.521951"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PKH60_02755"
     CDS             complement(<1..463)
                     /locus_tag="PKH60_02755"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNQ17001.1"
                     /translation="MKIVKKYGLNVGILVFCTIFSWWLMSSTFGYRDGSIVIDSKLYS
                     DFGAHLPLIRSFSLGWNFPPQYPFFIGEPIRYHYLFYLLIGLLERSGVRIDLAVNLLS
                     AGGFSLLLFMIYIFAKTLFRSSLAGVLAVILFLFNGSLAWVEFFNQHGWTWE"
     gene            complement(470..1771)
                     /locus_tag="PKH60_02760"
     CDS             complement(470..1771)
                     /locus_tag="PKH60_02760"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNQ17002.1"
                     /translation="MKIAIIGAGFTGLSAAWNLSQSGYDVSLFESSQFPGGLAAGFKP
                     DESEGEPWEWSLEHHYHHIFASDKSIINWVRELGLSRSLFFNSAKTSMRYDDRQFRLD
                     SPLSLLACPVLSPWSKIRTAFVLALLKTLPLWEQLEKFTVKEFLIRSMGSQSWSVLWE
                     PLMLDKFESHAETVNAAWFWARIHARTPKLGYFAGGFGQLAQDVVDRLQEKHVRCYFS
                     TSIRYAAQKNGQWQIEQEDGSSNFFDAVLFTSSAMLLSGLVPELPESYLAGILRLKAM
                     AARTLILELDKPFFTDNTYWLNVNEKSWPLLAVVEHTNMISPQHYGNKTIVYIGKYLD
                     PSNPMYSFSKEQLLDHYKPYLDQLSPGFLRSLSRSWSFAAPFAQPVVEVNHSYVLPPI
                     KTPLEGLYWASMQHIYPWDRGTNFAVKLGQTAAQTIRSNIQ"
     gene            complement(1782..2486)
                     /locus_tag="PKH60_02765"
     CDS             complement(1782..2486)
                     /locus_tag="PKH60_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNQ17003.1"
                     /translation="MQKKILIANWKSHKTKSEVDHWFSTLEKNYAEEKDVFDNLTIII
                     APSVLYLELGVNWSAKIPGISIAAQDVSQFPLGAYTGAVAARQLASVGVSYAIVGHSE
                     RRKYFGETHQIVAQKIDQLLEAKLLPIVCVDEQEIMQQADFIPKDQAKQCIFAYEPVS
                     AIGSGIGQDIPQVTASVKLAKQSFGEVPVLYGGSVDVRNIQEYLLVADGALIGGASLD
                     VAIFSQLLSSTVSKNS"
     gene            2537..4441
                     /locus_tag="PKH60_02770"
     CDS             2537..4441
                     /locus_tag="PKH60_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNQ17004.1"
                     /translation="MPNVLDALAKLNLEQKKAVIHGEGPAIVLAGAGSGKTTVLTSRA
                     AWLIEQQNVDPQSILLVTFTNKAAGEMRQRIAKLTKHYLPYVGTFHSFCARFLRNHSD
                     LIGFATPFTIYDANDQLSTMKLVYREHSIDPKQVNMRAVLAQISNAKNDLLTPSEYTG
                     VGRGYLQEQTAQLYTWYQQKLRDANAMDFDDLLFYTVEILHNHPDVKEKYQQQFYSVL
                     VDEYQDTNKVQYELTKVLTAPQNNLFVVGDFSQSIYGWRGADFRNMLRLTDDFSSVAE
                     YRLEQNYRSQQTILDAATQVIAANRMHPVLSLWTEKTESNPLVLKATRSDTEEASQVA
                     RWILHESAHTPLHDMAILYRTNAQSRVFEEQFLAAGIPYQIIGGTKFYDRKEIKDILS
                     YAHVLTNPQDIISFQRVEKVGKRKSRSFQQWVSSMKSQDITTLTPTEVLKGILQCTEY
                     AKQFDSNIPEDLSRLENIAELLRVATKFETMTDFLENIALIQDGYMHTAGGLKDSQGV
                     QLMSLHSAKGLEFTIVFLVGLEEGLLPHARSFMSPEDLEEERRLCYVGITRAKQKLFI
                     SYATTRYAFGSSAQNLPSRFLREISPSLFSKESSLLHQNKNSSERSIVSDDFLDQILS
                     GELDVQSFLE"
     gene            4454..5482
                     /locus_tag="PKH60_02775"
     CDS             4454..5482
                     /locus_tag="PKH60_02775"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNQ17005.1"
                     /translation="MIRPLREEEKALFNAVATHPLQSWEWGEFKKKTGQQIERVGFFS
                     NGKLTHTLQVSFHPIPIIGGTAGYLPKSFMPNEEQLQVLKQLGEKHAAVFIKMEPNVS
                     HPADSKTAFDQIMGFLEKNNAVAGRPLFTRYTFKIDLTQTEKELFANLSQKTRYNVRL
                     AIKKGVTIEENTSEAGMEHYLTILKETTKRQGFYAHSPEYFKTMWQTLGSSGMLKIFQ
                     ATYQNECIASWILFSLNNTFYYPYGASSSKYREVMASNLLMWEMIKYGKSIGCTEFDL
                     WGALGPEAKDSHPWAGFHRFKAGYGGTLMEYLGSFDLVLQPMQYKLFRIAENTRWKWL
                     RLRTKLGI"
     gene            5498..6238
                     /gene="pyrH"
                     /locus_tag="PKH60_02780"
     CDS             5498..6238
                     /gene="pyrH"
                     /locus_tag="PKH60_02780"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNQ17006.1"
                     /translation="MAQTRFKRILLKVGGESILGDRSYGIDPNASLSVAEQIRQIYTL
                     HVQIAIVIGGGNIFRGIAGSQGGIERATADYIGMLATVMNGLALQDALEKVGVPTRLQ
                     SALQMPAVAESFIRRRAIRHMEKGRVVILAAGTGVPFVTTDTGASLHALELHCNVLMK
                     ATKVDGVYDKDPNQHTDAKRFKTLTFKDAIEMDGVEVMDTSALAMSKDNNMPVMVFEL
                     FKEGNLVKAVLGEEIGTYVSNEVKTEFA"
     gene            6256..7305
                     /gene="gap"
                     /locus_tag="PKH60_02785"
     CDS             6256..7305
                     /gene="gap"
                     /locus_tag="PKH60_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNQ17007.1"
                     /translation="MPAQHNFGINGFGRIGRTAFRVWWNFHRERIDLKVINTSGSMEI
                     EDWLHLLRFDSNYGLFAEPISAQRVQSKKDASDENSLLGYFIIGDRKIAVTAQRDPKK
                     IPWREHQVQTVIESTGVFTTNEKARAHLESGASTVILSAPGKGDDISTSIIGVNAFDQ
                     NNPIRSNASCTTNCITPVAQVIHEMFGVAKATLTTIHSYTDDQNVQDNSHHDDLRRAR
                     SAAENIIPTSTGAAKATTLVIPELKGLFDGLAVRVPTPVGSLSDMVFLVKRKTSVEEI
                     NQALITAAQSDRWRGILAVTNYPIVSSDIVGRRESSIVDLPLTQVIDGDLVKIVSWYD
                     NEYGYCNRLVEQVNR"
     gene            7384..7809
                     /locus_tag="PKH60_02790"
     CDS             7384..7809
                     /locus_tag="PKH60_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNQ17008.1"
                     /translation="MSDSESATQTYHSYHELKVWQKAVELNQLVLKSLEELPTVTRNL
                     LGEHLVRACASIPAHIANGYASRSKTDYIDNLKSSLRQLSVVESIVLTQGTEGEFYQS
                     VAELLTELRKMLAGLTSSLTKPKKEIPKEEKETTTESSK"
     gene            7814..9325
                     /locus_tag="PKH60_02795"
     CDS             7814..9325
                     /locus_tag="PKH60_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNQ17009.1"
                     /translation="MVERTYSATLVGLEAVQIEVEVDGAPGTPNLILVGLPSRTVDEA
                     KDRITSAIQNSGVRIRSKRTVVNLAPASIKKSDSTLELAITIAILKMYGEITADTDST
                     MFFGELSLDGSLRGISGVLPLVQAAHSLSFQSVIVPQENAEEASVVDNIDILPANNLK
                     EILDHFRKIKPIVAKSITRYQPTPCVKSNDFQEIRGQLFAKRALEISAAGGHNIFLIG
                     SPGSGKTLLARAMINLLPPLTKAEAMEVTNLYSVSGLLIRKGLVTQRPFRSPHHSTSH
                     IGLIGGGSTIRPGEISLAHRGILFLDEFPEFSRASIEALRQPLEEGDITISRAMGSVR
                     YPARFILVAAANPCPCGNALSRASVCRCSPISRQRYQQRVSGPILDRIDLHVVVQPVP
                     IRELLPKNNQLESISTIVKRVQSARKIQTNRWQRFQILTNSELPSKLVREHCNLTNAA
                     KEMLDKAATTLRLTARAYFKVVKVGQTIADLVNSPTIEAIHVTEALQYRSISS"
     gene            complement(9334..10116)
                     /locus_tag="PKH60_02800"
     CDS             complement(9334..10116)
                     /locus_tag="PKH60_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNQ17010.1"
                     /translation="MSIFQSVVLGLVQGFTEFLPISSSGHLVLFSGLFKLEESSQLLF
                     FAMLHVATLVAVLLFFFRDLIKLRLKDYWIIGVGTLPALIIGLLFKDFLESLFVLPVL
                     NSVTLVMTGLINIAADKKMQTLPEESPAKSTLTPGWKQAIVVGIAQAISIIPGISRSG
                     STVATGVFMNMPRTQAFKFSFFLSIPAILGASLLEVADHISQTQSFSLTVAEWIGLLA
                     AFVGGLLSLKLFSLVIHKAQLKWFGYYVITVGILSGLYFVFL"
     gene            complement(10134..10820)
                     /locus_tag="PKH60_02805"
     CDS             complement(10134..10820)
                     /locus_tag="PKH60_02805"
                     /inference="COORDINATES: protein motif:HMM:NF017373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17011.1"
                     /translation="MNSLSPNLYTFSTELAYFFPKLIFGVVLFIIGLILAKMVRRLVV
                     RVIRMLMNSSLVTNTPIEHFFSNGDLTHKLDEGFGSVAYWFMLLLTAYVVSATVGLTS
                     VSYLLEQVFHYVPRFISATIVLIIGVLLAGLVESFSKSSIGKLDPKAGKVVSKLSSYL
                     VMVLTLMVAFSELGIARDFILILFVGLVLAIAMGIGLAVGLGGQHAVKQLTESWMSDV
                     KKDTGIKEKK"
     gene            complement(10856..11215)
                     /gene="rnpB"
                     /locus_tag="PKH60_02810"
     ncRNA           complement(10856..11215)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKH60_02810"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(11409..12368)
                     /locus_tag="PKH60_02815"
     CDS             complement(11409..12368)
                     /locus_tag="PKH60_02815"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNQ17012.1"
                     /translation="MKIDRLNNCISIVGPTATGKTERALEVAHQLINENKVAGVDIIS
                     ADSRQVYRGLEVLTGADVPAGFMGSITPSLSKSGLPYFTNSEGSIHIFGISMINPAEE
                     WSLAHFVNFAREVMQWSWSNGRQPILVGGTGLYHKHLFSDDKTPQIPQDEDFRIEAQK
                     KSVEELRQKLNKIDVNRLDQLNESDRENPRRLIRAIEVAIWQQKHRSSSLLQSGSTES
                     PVVWSQVDNQMEFVSSSLKIIEERIEQRVQKRLTSGAIQEVRDLLKLNLPENSQVLTT
                     LGVPEISQYLEGSLSADDCQKLWSLHEFQYAKRQLTWWKKTLG"
     gene            complement(12365..12784)
                     /locus_tag="PKH60_02820"
     CDS             complement(12365..12784)
                     /locus_tag="PKH60_02820"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNQ17013.1"
                     /translation="MNEPNIGVPIFFTRNNQLLLGQRKNSFGSGLFGLPGGRVAPQET
                     LEQCLIREAKEEVGISPIKFKSIGLVKEWQATHFFLHFIFVCTKWKGEIANLEPEKSE
                     NWQWYSLDSLPEDILSGHKAGIDMFLTNARQFQVVEI"
     gene            complement(12800..13951)
                     /locus_tag="PKH60_02825"
     CDS             complement(12800..13951)
                     /locus_tag="PKH60_02825"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNQ17014.1"
                     /translation="MPTYFIHTFGCQSNKSDSERIAGDYQARGYFEATDWQTCDELVV
                     NTCAVRQSAEDRARGFLHTVGVYFNEHSKKRPKIILTGCMVHHGNEKLYAMMPIIDEI
                     LPINEVGFNQAAIRKDTKHAWIPISTGCNSFCTYCIVPYSRGREKSRSFESIMEEVDR
                     LVGQGFEEITLLGMNVNSWGLEKVGIGFRKLMMEKADFNREDLPDNQSQYLKPKETPP
                     FVKLIRAISAFDQIKTIRFMTSNPWDFHDELIAEIGVNKKIDRYVHLPVQSGSNAVLY
                     RMNRGYTRESYIEVVKKLRAVDPEIVIGTDIVVGFASETEAEFQETVDLAKQMDWHVG
                     FVAMYSPRPGTASWRIYPDDIPHKEKKRRWEILDQIINKDNLKIRPKVV"
     gene            complement(14000..14638)
                     /locus_tag="PKH60_02830"
     CDS             complement(14000..14638)
                     /locus_tag="PKH60_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNQ17015.1"
                     /translation="MIQKLIIPRQKQINTGLLIFELFLLVIWCLAVFVALNYPQQVFV
                     VLKISGKLGTASLLLFSLTLLPGIIMRLKVIPKITLPIATLITPFRRHLGILMFITAF
                     VHMSFSSTLPYIALQLISTGKVVLPPPFRLFEQIGLIGWMLLLPVWLTSNDFSMKKLG
                     KWWKRLQRLTYLALWLIFAHVAMQSQPVAVLLLVVGLLEMYSWVLVWRRKQK"
BASE COUNT         4576 a         3268 c         2939 g         4055 t
ORIGIN      
        1 cttcccatgt ccatccgtgc tgattaaaaa attctaccca tgcgagcgat ccattgaaaa
       61 gaaaaagtat tactgctagg actccagcta atgaagagcg aaaaagcgtt ttagcaaaga
      121 tataaatcat aaatagtaat agactaaatc ctcctgcgct caacaggtta acagccaaat
      181 caattctgac accagatctt tcgagtaagc cgatgagtag ataaaacaaa tagtggtaac
      241 gaattggttc accaataaaa aatggatatt gaggcggaaa gttccaacct agactaaacg
      301 atcgtatgag tggtaagtgg gctccaaaat cactatatag cttgctatct atcacaatcg
      361 agccatcccg ataaccaaaa gtactgctca taagccacca agaaaagatc gtgcagaaaa
      421 ctaaaatacc aacattcaaa ccgtattttt tgacaatttt cattgatttt tattgtatgt
      481 ttgatctaat agtttgagcc gcagtctgac ccagttttac agcaaaattt gtacccctat
      541 cccatggata aatatgttgc atgcttgccc aatataaacc ttctagaggc gtcttaattg
      601 gtggcaacac ataactgtga ttaacctcaa cgactggctg ggcaaatgga gcagcaaaag
      661 accatgagcg agacagactt ctcaaaaaac cgggtgataa ttgatctaag tatggtttgt
      721 agtgatctag caactgttct ttggaaaatg agtacattgg attgctagga tctaggtact
      781 taccaatgta tacgatagtt ttattcccat aatgttgtgg gctaatcata tttgtgtgtt
      841 ctactaccgc gagtaatggc caagattttt catttacgtt taaccaatag gtgttatctg
      901 taaaaaatgg tttatctaat tccaaaatca gggttctggc cgccatcgcc tttaatctaa
      961 gtattccagc caaatatgat tctggcaact ctggcactaa tccagataaa agcatggcag
     1021 aactcgtaaa caacacggca tcaaaaaaat tacttgatcc atcttcttgc tcgatctgcc
     1081 actgaccatt tttttgagca gcataccgga tgctggtgga aaaataacat cgcacatgtt
     1141 tctcttgcaa cctatctacc acatcttgcg ccaactgtcc aaatccacca gcaaaatatc
     1201 ctaatttggg tgttcgagca tggattctag cccagaacca agctgcatta acagtttccg
     1261 catgcgattc aaatttatct agcatgagtg gttcccacag cacactccat gattgacttc
     1321 ccattgatcg aattaaaaat tcctttacgg tgaacttctc taattgctcc caaaggggaa
     1381 gagtttttag taacgctagg acaaaagcag tgcgaatttt tgaccatgga gaaagcacgg
     1441 gacaagcaag taagctaaga ggtgagtcga gtcgaaattg tctatcatca tatctcattg
     1501 aagtttttgc cgaattaaaa aaaagtgaac gagatagtcc aagctcgcgc acccagttaa
     1561 taattgactt atcggaagca aaaatgtgat ggtagtggtg ctccaatgac cactcccagg
     1621 gttctccttc tgattcatcc ggtttaaacc cagctgcgag gccgcctggg aattgacttg
     1681 attcaaacag cgagacatca taaccacttt gtgaaagatt ccaggcagct gatagaccgg
     1741 taaaacctgc tccgatgatt gcgattttca tgcagtatat cctaagagtt cttactaaca
     1801 gtcgaactta ataactgaga aaatatggct acatcaagac tagctccacc aattagagct
     1861 ccgtcggcaa ccagtaaata ctcttggata tttctcacat caacactccc tccgtacaaa
     1921 actggtactt ctccaaaaga ttgcttagct aatttaactg aagcagtaac ttggggaata
     1981 tcttggccaa taccactgcc aattgcggaa accggctcat acgcaaaaat acattgtttg
     2041 gcttgatctt ttgggatgaa atcagcttgt tgcatgattt cttgctcgtc cacacaaact
     2101 atgggcagca gcttcgcttc aagtaattga tcaatttttt gcgcgacaat ctggtgcgtc
     2161 tctccaaagt attttcttcg ctcactatga cctacaatcg catatgaaac tccaacgcta
     2221 gccagctgac gagccgcaac tgctcccgtg tatgctccga gcggaaattg acttacgtct
     2281 tgtgcggcaa ttgaaatacc tggaatcttt gcactccagt taactcctaa ctctaaatat
     2341 aaaactgaag gagcgataat aatagttaaa ttgtcaaaaa catctttttc ttccgcatag
     2401 tttttttcta aagtggaaaa ccaatgatcc acctccgact tggttttatg agatttccaa
     2461 tttgcaatga gtatcttttt ctgcatgctc ttattaaacc acactcgatg agagttttct
     2521 gttgcaatgt gttactatgc ctaacgtgct agatgctctt gccaaactca atctcgaaca
     2581 aaaaaaagct gtcattcatg gtgaaggacc tgcaattgtg ctcgctggtg cgggttctgg
     2641 aaaaaccaca gtcctaacct ccagggcagc ctggctaatt gagcaacaaa acgttgatcc
     2701 ccaatcaata ttgttggtca ccttcacgaa taaagcggcg ggtgaaatgc gtcagcgaat
     2761 agcaaaatta accaaacact acttgccata cgtaggtact tttcacagtt tttgcgcgcg
     2821 atttctcaga aatcacagcg atctaattgg atttgcgaca ccctttacca tttacgacgc
     2881 taatgatcaa ttatcgacta tgaagttggt ttatcgagaa cattcaatcg atccaaaaca
     2941 agtcaatatg cgcgcagtac tcgctcaaat ttctaatgcc aaaaatgatt tactaacgcc
     3001 gtcagagtat acgggtgtag gtcgaggcta tcttcaagaa caaaccgctc aactatatac
     3061 ttggtatcaa caaaaattga gagatgctaa tgcaatggat tttgatgatt tactttttta
     3121 tacagtagaa attttgcata atcatccaga cgttaaggaa aagtatcaac aacagtttta
     3181 ttccgtactc gttgatgaat atcaagatac aaataaagtt caatacgaac taaccaaagt
     3241 gctgactgca ccacaaaata acttgtttgt ggtgggtgat ttttctcaga gtatttatgg
     3301 atggagagga gcagacttcc gcaacatgct tcgactcaca gatgacttta gctcggtcgc
     3361 tgagtaccgc ttggagcaaa actaccgttc ccaacaaaca attttagacg ctgccactca
     3421 ggtgattgct gccaatcgca tgcacccagt cttatctcta tggaccgaaa aaactgaatc
     3481 aaacccccta gtgcttaaag ctactcgctc tgatacagag gaggcatctc aagttgcgcg
     3541 gtggatactt catgagtcag cccacacacc ccttcacgat atggccatcc tctacagaac
     3601 aaatgcccaa tcacgtgttt ttgaagaaca attcttggct gcaggaattc catatcaaat
     3661 aattggcgga actaaatttt atgatcgaaa agaaattaaa gatattttga gctatgcaca
     3721 tgtgctaaca aacccgcaag atatcattag ttttcaacgg gttgaaaaag tgggaaaaag
     3781 aaaatctcga tcctttcaac aatgggtatc tagcatgaag tcgcaagata taacaacact
     3841 aaccccaact gaagtactaa aaggcattct gcaatgcacg gaatatgcca aacaatttga
     3901 ttcaaatatt ccagaagacc taagtcgact tgaaaacatt gccgaattac ttcgggtggc
     3961 aacaaaattt gaaacgatga ccgatttttt agagaatatc gcccttattc aagacgggta
     4021 catgcacaca gctggaggac tcaaagatag tcagggtgtt caattaatga gtctgcactc
     4081 agcaaaggga ttagaattta cgatcgtatt ccttgttgga ttggaagaag gactactacc
     4141 tcatgctcga tcatttatga gtccggaaga tcttgaagaa gagcgtcgat tatgctacgt
     4201 aggtatcact agagcgaaac aaaagttgtt tattagttat gcaacaacgc gatatgcctt
     4261 tggctcatct gcacaaaatc tccccagtcg attcttaaga gaaatttcgc cttcgttgtt
     4321 ttcaaaggaa tcaagtttgt tacaccaaaa caaaaactca agcgaacgaa gtatcgtaag
     4381 cgatgatttt cttgatcaaa ttttatccgg agaactagac gtacaaagtt ttttagaata
     4441 aaatgagcct gttatgattc gacctctcag agaagaagaa aaagctctct ttaatgctgt
     4501 agcaacgcac ccattacaat catgggagtg gggagaattt aagaaaaaaa ccggccaaca
     4561 aattgaacga gtgggatttt tttccaatgg taagttaact cacacgctgc aggtcagctt
     4621 tcatccaatc ccaattatcg gtggaactgc cggctatctg cccaaatcct tcatgccaaa
     4681 tgaggaacag ctacaagtac tcaagcagct aggagaaaaa catgcagccg tatttattaa
     4741 aatggaacca aatgtttctc atcctgccga ctcgaaaacc gcctttgatc aaatcatggg
     4801 ttttcttgaa aaaaacaatg cggttgcggg cagaccactt tttaccagat atacttttaa
     4861 aattgactta acacaaacag aaaaagaatt atttgctaat ctctcacaaa aaactcgcta
     4921 caatgttcgc ctagcaatta aaaaaggagt caccattgaa gaaaatacct cagaagcagg
     4981 catggaacac tatttaacca tacttaaaga aacaacaaaa cgtcagggct tttatgccca
     5041 ttctccagaa tactttaaaa caatgtggca aaccctcggt tcaagtggaa tgttaaaaat
     5101 tttccaggct acgtatcaaa atgagtgcat tgcaagttgg attctgtttt ccctcaataa
     5161 cactttttat tatccttacg gcgcatcttc ttcaaagtat cgagaggtaa tggcaagcaa
     5221 tcttcttatg tgggaaatga taaagtatgg gaagtcaatc gggtgtactg agtttgatct
     5281 ctggggagcg cttggaccgg aggccaaaga tagccacccc tgggcaggtt ttcatcgttt
     5341 taaagctggt tacggtggta cacttatgga atacttaggt agtttcgatt tagtgctaca
     5401 accaatgcag tataagcttt tcagaattgc tgaaaatact cgctggaaat ggctaagact
     5461 tagaactaaa ttgggaatat agactgaaag gaatcctatg gctcaaacaa gatttaaacg
     5521 aattctgctt aaagtaggag gagaatctat tcttggagat agatcgtatg gcatcgaccc
     5581 gaacgcatca ttgtcggtgg cagaacaaat tcgtcaaatt tacacacttc atgtccaaat
     5641 cgctatagtg attggggggg gcaatatttt tcgaggtatc gcgggttcgc aaggtggtat
     5701 tgagcgagca actgcagatt atattggaat gttggcaact gtcatgaatg gcttggctct
     5761 tcaagatgcg ctagaaaaag tgggcgttcc caccagactt caatcagcat tgcagatgcc
     5821 tgcagttgct gaaagcttta tccgcagacg agccatccgc cacatggaga agggtagagt
     5881 ggtcattttg gcagcaggca ctggtgttcc ctttgtcacc acggatactg gagcttctct
     5941 tcacgcacta gaactacatt gcaatgtgct catgaaggcc actaaggtag atggggttta
     6001 tgataaggat cccaaccaac acaccgatgc aaaacgtttt aaaactctca catttaaaga
     6061 tgcaattgaa atggatggtg tcgaagtcat ggataccagt gcattagcta tgagtaaaga
     6121 taacaacatg ccagtaatgg tctttgaact tttcaaagag ggcaatttag tcaaagccgt
     6181 cttgggtgaa gagatcggca catacgtgag taacgaagta aaaactgagt ttgcataaat
     6241 taataaggag tcgctatgcc cgcccaacat aattttggta tcaatggctt cggccgtatt
     6301 ggacgtacag cgtttcgcgt ctggtggaac tttcatcggg aaagaattga tttgaaagtc
     6361 ataaatacct caggatcaat ggaaattgag gattggctgc acttgctcag atttgatagc
     6421 aattatggat tatttgccga accaatcagc gcgcaacgcg ttcagtcaaa aaaagacgca
     6481 agtgatgaaa acagcctgct tgggtatttt atcatcggtg accgaaaaat tgccgtaact
     6541 gcccagcgag atccaaagaa aatcccgtgg cgcgagcatc aggtccaaac agtcattgag
     6601 tctaccggtg tgtttaccac aaatgaaaaa gcccgcgccc acttagaaag cggcgctagc
     6661 accgtcattc tctctgctcc aggtaagggt gatgatattt ctacctccat cattggagta
     6721 aatgcatttg accaaaataa tccaattcgt tctaatgcat catgcaccac caactgcatt
     6781 actccagtgg cacaagtcat tcatgagatg tttggtgttg ccaaagcaac gctaacaaca
     6841 attcattcct atactgatga tcaaaacgtg caagataact cacatcatga cgatttgcgc
     6901 agagccaggt ctgcagccga aaatattatt ccaacctcaa ctggagctgc aaaagctaca
     6961 acactagtta ttccagaact caaaggtttg tttgacggtt tagcagtccg agtaccaact
     7021 ccagtcggct cactgtctga catggttttc ttggttaagc gaaaaacctc ggtagaagaa
     7081 atcaatcaag ctctcatcac cgcagctcag tcagatcgct ggcgagggat attagcagtc
     7141 accaactatc caattgtttc ttccgatatc gtgggaagac gagagtcgag cattgtggat
     7201 ttaccactca ctcaggttat tgacggcgat ttagtgaaga tagttagttg gtacgataat
     7261 gaatatggct actgcaatcg cttggttgaa caggtgaata gataatgatt ccagataaag
     7321 ttgtacgctt aatgtaagct acctccatat gatcttcctt agtactacat accaaggagg
     7381 catatgtctg attcagaatc agccacccaa acgtatcact cataccatga gttaaaagtt
     7441 tggcaaaaag cagttgagtt aaatcagctg gtattaaaat cacttgaaga gttaccaacg
     7501 gtgaccagaa acttgctcgg cgaacaccta gttagagcct gcgctagtat tcccgctcac
     7561 attgccaacg gttacgcttc gcgttcaaag acagattata tagataatct taaatccagc
     7621 ttgcgccaac tttctgtcgt ggaaagcatt gtgcttaccc agggtacgga gggagaattt
     7681 tatcaatcgg tagctgagtt gcttaccgaa ttgagaaaaa tgctagcagg attaactagt
     7741 tcactcacta aaccaaaaaa agaaattccc aaggaggaga aagaaacgac tacagagagc
     7801 agcaaatagc gctatggttg aacgaaccta ttcagctacg ctggtgggac ttgaagcagt
     7861 acaaatcgag gttgaggttg atggggcgcc cggtactccc aacctcatcc tcgttggttt
     7921 acccagtcga accgttgacg aggcaaaaga tcgcatcact tctgccattc aaaactcagg
     7981 agtaagaatt cgcagcaaac gaactgttgt taatctcgca ccagcatcga taaaaaaatc
     8041 agacagcaca cttgagttgg caattacgat tgccattttg aaaatgtatg gcgaaattac
     8101 tgctgacact gactccacca tgttttttgg tgaactatca ctggatggat cgctgcgagg
     8161 aattagcgga gttttacctc ttgtgcaagc tgctcattca ttgtcttttc aatctgttat
     8221 cgtgccccaa gaaaatgctg aggaagcaag tgtggttgat aacatcgata tcttacctgc
     8281 caacaatctc aaagagatcc tagatcactt tcgtaaaata aaacctattg tggcaaaatc
     8341 aattactcgt tatcagccga caccctgcgt taaatccaat gactttcaag aaattcgcgg
     8401 tcaattattt gccaaaagag ccttagaaat tagcgcagca ggtggccaca acatattttt
     8461 aataggatca cctggatcag gaaaaacgct attggcaaga gccatgatca atttactgcc
     8521 acctctgaca aaagctgagg cgatggaagt tacgaatctg tactcggtga gtggattact
     8581 cattaggaaa ggactcgtaa cccaacgacc gtttcgttca ccacaccata gtacttccca
     8641 cattggactg attggtggtg gcagtacgat tcgtccagga gaaatttcac tggcacatcg
     8701 tggtatttta tttctcgacg agtttccaga attttctaga gcatcaatcg aggctctgcg
     8761 gcaaccacta gaagaagggg acataactat atctagagcg atgggatcgg ttcgatatcc
     8821 cgctcgattt attttggttg ccgctgctaa tccatgtccc tgtggcaatg cgctgagtag
     8881 ggcgagcgtc tgccgatgct cgcccatcag tcgtcaacgc tatcaacaac gcgtatctgg
     8941 tccaatccta gataggatag atttgcatgt agtggtacaa cccgtaccaa ttcgcgaact
     9001 tttaccaaaa aataatcagc ttgaatcaat cagtactatc gtcaagagag tgcaatctgc
     9061 gagaaaaatt caaacaaatc gctggcaacg ttttcagata ctaacaaata gcgagttacc
     9121 atcaaaactg gtacgagaac actgcaatct cactaacgct gcaaaagaaa tgttggataa
     9181 ggcagctaca actctgagat taaccgcccg agcttacttt aaagttgtta aggttggtca
     9241 aactattgcc gatcttgtta attctcctac aattgaagca atccatgtta ctgaagcact
     9301 tcagtaccga tcaatctctt cctaggcaac attctagaga aaaacaaagt ataaaccact
     9361 caaaataccg acagtaataa cataataacc aaaccatttt agttgtgctt tatgaatcac
     9421 taatgaaaat agttttaagc tcagtagtcc accaacaaat gcggcaagca atccaatcca
     9481 ctcagcaacg gtgagagaga acgattgagt ttgagaaata tgatccgcta cttctagtaa
     9541 actagcccca agaatggcag gaatcgacag gaaaaatgaa aatttaaacg cctgcgttct
     9601 tggcatattc ataaacaccc cagtggctac agttgatcct gagcgggaaa tgccaggaat
     9661 aatcgaaatc gcttgagcaa taccaacaac tattgcctgt ttccatcctg gagttagtgt
     9721 agattttgcc ggagattctt ctggtagagt ttgcattttt ttgtccgccg caatgtttat
     9781 caaaccagtc atcactaagg tgacactatt taacaccggt aaaacaaaaa gtgattcaag
     9841 aaaatcttta aaaagaagac cgataatcag cgctggaagc gtacctacac caattatcca
     9901 gtagtctttt aaacgcagtt taatcagatc acgaaaaaaa aatagtaaca ccgccaccag
     9961 tgttgccaca tgcaacatcg caaaaaataa cagctgcgat gattcttcga gtttgaaaag
    10021 cccactaaat aggaccaagt gtccagaact tgagatgggt aagaactctg taaatccctg
    10081 aactaatccc agaacgaccg attgaaaaat tgacatgctt gaaaccaatt aaattatttt
    10141 ttttctttaa ttccggtgtc ttttttgaca tcactcatcc agctttcagt tagttgcttg
    10201 actgcgtgtt gtccaccaag acctactgct aaaccaatcc ccatggcaat tgctaaaact
    10261 aagccaacaa aaagaattag aataaagtct cgcgctattc ctaattctga aaacgcaacc
    10321 attagagtta ataccattac caggtagcta ctcaatttgc ttactacttt gcctgcttta
    10381 ggatcaagct tgccaattga agatttgcta aaactttcga ctaatcccgc aagtaaaaca
    10441 ccaataatca acactatggt cgctgatata aatcttggca catagtgaaa gacctgttcg
    10501 agaagatatg atacagaggt caaaccaacg gtagctgaaa caacataggc tgtgagcaac
    10561 aacatgaacc aataggcaac actaccaaaa ccctcatcaa gtttatgggt aagatctccg
    10621 ttactaaaaa agtgttcgat tggagtattc gttacaagag aagaattcat tagcatgcga
    10681 atgactctga ctaccagtcg cctgaccatt tttgccaaga ttaaaccaat aatgaagaga
    10741 actactccaa aaattagttt ggggaaaaaa taggctagct ctgtactgaa tgtatataag
    10801 ttgggggaaa gtgaattcat agtatctcat tatagatgac tttactaata actgaaagct
    10861 gtgcaatccg ataagccaga ttctgtcgag gatagtgatc tatctatgcc cgcgacatta
    10921 tcaccgtcag aaggctcggc cgaaacgtcg ccctctgatg cgagtgtgtg caatgcggtg
    10981 aagttcggcg gttgcagcgg ggaggattgt catcatgctc gatcgaaacc gagtagagga
    11041 tacccaatag cttggtcgtt tcacctggcg ctacccagct cgtcactgtt gctctgactg
    11101 ttgtgctcgg cttacgcctc gcaccctcca cttttcagtg gacaccctac actctgctgt
    11161 ccggactttc ctcccagccg ctcgcgagcg gctgggccac tacctagatt acacagcttg
    11221 cagatattat agcagtctgg atcaataaat tatggaaaaa attattctcc cgtgcatttc
    11281 aatgaagcag actccaaaat tcgtttaccg agatacgcag gaatgcttga ttggagaatg
    11341 tctgaaactc aaggcattag tgatgaaggg tatttcttgt taattttggt ctatgagctg
    11401 aacgaagact aacctagtgt ttttttccac cacgtcaact ggcgtttagc gtattgaaat
    11461 tcgtggagag accaaagttt ttgacaatcg tctgcagata gagatccctc aagatattga
    11521 ctaatttctg gaactcccag ggtggtcaaa acttgactat tttcaggcag atttagtttg
    11581 agcaaatctc gcacttcttg tatcgcgccg ctggttaacc gcttttgtac ccgctgttct
    11641 attcgttctt cgattatctt cagcgacgaa gaaacaaatt ccatttgatt atccacttgg
    11701 ctccacacga caggcgactc agtagaccca gactgtaaca acgaactgct ccgatgtttc
    11761 tgttgccaaa ttgccacttc aattgcacga atcaaccgtc gaggattttc tctgtctgac
    11821 tcattgagtt gatctaatct attcacgtca atcttgttca acttttgtcg taattcttca
    11881 accgactttt tctgagcctc aattctaaaa tcttcatcct gcggaatttg tggagtcttg
    11941 tcatctgaaa ataaatgttt gtgatacaac cccgttccgc ccacaagtat tggttgacga
    12001 ccatttgacc agctccattg cattacttct ctagcaaaat taacaaaatg agcaagtgac
    12061 cactcttcgg ctggattgat catcgagata ccaaaaatgt gaattgagcc ttctgagtta
    12121 gtgaagtagg gtaggccact cttcgacaag ctgggagtga tactccccat aaatcccgcc
    12181 ggtacatctg caccagttaa aacttccaaa cccctgtaca cctgtctcga atctgcactg
    12241 attatatcga ctcctgcgac tttattttca tttatcaatt gatgagccac ctccaacgcc
    12301 cgctcggtct ttcccgttgc tgtaggacca acaatagaaa tacagttgtt caagcgatcg
    12361 attttcatat ttcgactacc tggaactgcc gtgcgttagt caaaaacatg tctattccag
    12421 ccttatgccc tgacaaaata tcctcaggca agctatctaa ggaataccat tgccaatttt
    12481 cagatttctc tggttcgaga tttgcgatct cacccttcca tttagtacaa acaaaaataa
    12541 agtgaagaaa aaaatgggtt gcttgccatt ctttcacaag cccaattgac ttgaacttga
    12601 ttggggatat tcccacttct tcctttgctt ccctgatcaa acattgctcc agtgtctcct
    12661 gtggtgccac tctaccacct ggcaatccaa acaaaccact cccaaaagaa tttttccttt
    12721 gtccaagaag taactgattg tttcttgtga agaaaattgg aactccaatg tttggttcgt
    12781 tcattctctg aattgtaacc taaactactt ttggtcggat tttcagatta tctttgttga
    12841 taatctgatc tagtatttcc catcttcgct tcttttcttt atgaggaata tcgtctgggt
    12901 aaatccgcca gctagcggtg cccgggcgag gggagtacat ggccacaaaa cccacgtgcc
    12961 aatccatttg tttggctaaa tcgactgttt cttgaaactc cgcttcggtt tcactagcaa
    13021 aaccgacaac aatatctgtg cctatcacaa tttctggatc aacggcacgc agttttttta
    13081 cgacttcaat ataactttcc ctagtatacc cacgattcat tcgatacaac acagcgttgc
    13141 tgccagactg cactggcaga tgcacatagc gatcaatctt cttatttaca ccgatttctg
    13201 caatcaattc gtcatgaaaa tcccacgggt tactagtcat gaaccgaata gttttaattt
    13261 gatcaaaagc cgaaattgcg cgaattaatt ttacaaaagg tggggtttct tttggcttca
    13321 aatattggga ttggttatct ggcaaatctt ctcgattgaa atccgctttt tccatcatca
    13381 acttacgaaa gccaatgcca actttttcta gaccccatga gttgacgttc ataccgagaa
    13441 gagtgatttc ctcaaagcct tgaccaacca ggcgatcaac ctcctccata atactttcaa
    13501 atgatctaga cttttctctt cccctggagt aggggacaat acaatacgtg caaaacgaat
    13561 tacaacctgt tgaaatagga atccaagcgt gcttagtatc tttccgtatg gccgcttgat
    13621 taaagcccac ctcattgatc ggcaatattt catcaataat tggcatcatg gcatacaatt
    13681 tttcattgcc atgatgcacc atacagccag ttaaaataat ctttggtcgt tttttgctat
    13741 gttcattaaa atacactccc accgtatgca aaaaacctct agcgcgatct tccgctgatt
    13801 ggcgtactgc acaagtattc actacaagct cgtcacaggt ttgccaatct gtggcttcaa
    13861 aatacccacg agcttgataa tcgccagcaa ttctttcgct atccgactta ttcgattgac
    13921 aaccaaaagt atgaataaaa tatgttggca tgaaggtgta gtgtaacatc cttgtcacac
    13981 tgagcgcatc gaggcgtgac tacttctgtt ttcgtcgcca aaccaaaacc caagagtaca
    14041 tctctagcaa tccaaccact aacaataata ctgcgactgg ttgagactgc atcgctacat
    14101 gggcaaagat gagccacaaa gctaaatatg ttaggcgttg caatctcttc caccatttac
    14161 ccaacttttt catcgaaaaa tcattagaag tcagccacac tggcaataat aacatccagc
    14221 caatcaaacc aatttgctcg aataaacgga acgggggagg aagtacaact ttaccagttg
    14281 agatcaactg gagcgcaata tatggcaggg tacttgaaaa actcatgtgc acaaacgcgg
    14341 tgatgaacat aaggatacct aagtgacgac gaaagggagt aattaacgtc gcaattggta
    14401 aggtgatttt aggaatcact ttcaaacgca tgataatgcc gggaagcaat gtgagtgaaa
    14461 agagaagcag tgatgcggtc cctaacttcc cgctaatctt gagcactaca aatacctgct
    14521 gagggtaatt gagcgcaaca aatacagcca aacaccaaat tactagtaaa aatagttcaa
    14581 aaatgagcag acctgtgttg atctgtttct gtcgaggaat tattaacttt tgaatcaatc
    14641 gcaagtattg tagtagtgct ttgaaaaccg aatgtaacat acattattaa tcaattatct
    14701 cataaagctt taagttcgaa ctcagttccg ccagcaggat aatcaaaacg aacggcggca
    14761 gttttttcac tcatgtgatg cacgcgcagg aaaagttcca caaatggatt atcgtatccg
    14821 cttgagccag cattgaca
//
LOCUS       DAOQZP010000027        14171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_4768_length_14171_cov_5.163219, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000027 DAOQZP010000000
VERSION     DAOQZP010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 14171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14171
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4768_length_14171_cov_5.163219"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /gene="serS"
                     /locus_tag="PKH60_02835"
     CDS             <1..1236
                     /gene="serS"
                     /locus_tag="PKH60_02835"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNQ17016.1"
                     /translation="AYIRNNTQLVKQASINKQLNPAIVDELLTVDEQRRALQQKVDDL
                     RRDSNEHARKLKQNVANGTLPTDEDKNLGKEFKNKLKELEPQLKEVEDKYQAMLYQIP
                     NVPANDVPVGPDESGNQMIRQVGKPKTFDFDVKPHEEIMTKLGWLDVDRAVRIGGFRS
                     YFLKGDGLRLEQALLRVALDRLVTAGFEPMSVPVLVNEDAMWGTGYFPWGQEDHYKTQ
                     DNQFLTGTAEVALTAYRQGETLTEKELPFQICGISPCFRREVGSYGKDTSGVIRVHQF
                     NKVEQVVYTVADETITREWHEKMVRLAEQLLEDLELPYQVLLMCTGDMGAGQRKKYDI
                     ETWFPSQQKYRETHSASYFNDFQSRRLNIKYQAKDGSTKFVYTLNNTMAASPRLLAAI
                     IENYQQPDGSVAIPKLLTF"
     gene            1277..4234
                     /gene="secA"
                     /locus_tag="PKH60_02840"
     CDS             1277..4234
                     /gene="secA"
                     /locus_tag="PKH60_02840"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNQ17017.1"
                     /translation="MFKFLTSIFDDNKKAINRFSAQVDQINRLEPEIKKLSDKALAQQ
                     TKKFQDQIETELKSGKSEKAVLNSILPEVYATVREVSRRVIGLRHYDVQLIAGIALHE
                     GTITEQKTGEGKTLTVTLPLYLNALLGKGAHLVTVNDYLAETGAGWMGPIYHFLGLTT
                     SVITHEKASLYDPDFTGEDRGDERLEHFKPISRTEAYAADITYGTNNEFGFDYLRDNM
                     VQSLSQMVQRPNNTHFYAIVDEADSILIDEARTPLIISAPDSEPTKKYYDFAHMVTSL
                     SKGADYTVDEKARAVTLTDLGVKRLEQKLNVKNLYEENFDTIHHVESALKANTIFQRD
                     KDYIVRDNQVIIVDEHTGRLMYGRRYSDGLHQAIEAKENVPIQQESRTLATISIQNYF
                     RLYSKLSGMTGTATTEAEEFRKIYAIDVIAVPTNDTVIRVDKTDVVYKTAGAKYTAIA
                     NEIETIHKEGRPILVGTRSINHNQIVADFLSRKKITHQVLNAKNHEKEAFIIADAGKK
                     GAITVATNIAGRGVDIVLGGAKPELKDYRKKTESKVKATADQIKLAKTLNLPPSIDPT
                     QYKLKEYQQAMEKWQSDHDEVVRLGGLYILGTERHESRRIDNQLRGRAGRQGDPGTSQ
                     FFISLDDEIMRIFGGEQISKIMDMLKIDEGQPIEHGMISKSIESAQVKVEGFFFDQRK
                     RLVEFDDVMNKQREITYIRRRRLLEEGSVLEKGEKITAEQEEVVAGLRQQIQGYVDEE
                     IRALVSLNAPKEFTAEEYATIVKEFCRLVPFDQASQLRLEKEISKITDPEEITEKLQT
                     ISQQLYDQREKDLGEMTMRQLELYVTLSTLDEKWMDHIDAIDSLREGIWLRGDKQTVL
                     SEYKKEAFGMFQQMLGAVDSTIANRIFRLQPISREQFRATQQKQLIEQKDDINEELSK
                     EVADATIPSSAPTQIKGDTQDLAAALRSAKAVAKPQPGSKQMKIGRNDPCPCGSGLKY
                     KKCGLVGAPEHRR"
     gene            4245..6011
                     /locus_tag="PKH60_02845"
     CDS             4245..6011
                     /locus_tag="PKH60_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ17018.1"
                     /translation="MEIDQRYSIHQFVIDVARYIRPYRTQFLIGIFLRITSDASRLYP
                     AWALSRIIELLTNSGTNTDPEELLRIFVLWGLAYAYFGLTHNLSKFFGYQVAEKASLD
                     IYKETLAHIFTLDFSWQEKENSGNKMKRIDKGLDGINITIRRIFDTLLSVLVNTVGIV
                     FIFFGLDPTLSVLLVLFMITYYVFSTLLLKRSIRQEKIVNKAFENLSGITFEALNNIQ
                     TIKALSIDWGVNESVNSHIKKLIQNIKKRIFYYQSQGALQVIYQSVFFLSIVAWLSWD
                     IYHGTKNVGLLVLFFGLFNKVSEATEELTNVTQQLALAKIWISRAMNILKTKPEIEDP
                     KKVSLQQTYPPDWKTIRIEKLGFGYGKKSALNEISLTIRRGEKIGIVGLSGAGKSTLF
                     KLLLDLYEDYDGHILIDNIPLKNLQRQSYIDHVAVVLQDTELFDMSLKDNIQIAGVPG
                     ASRQINLTEVIKMAHLESVVDQLENGVDSIVGEKGIKLSGGQRQRVGIARALYRQPDI
                     LLLDEATSHLDAHSEKEIQQALQENLHKFTTIVIAHRLSTIIKMDRIVVLSYGKIVEQ
                     GSFQELLNNQGAFSKMWKEQKI"
     gene            6064..6960
                     /locus_tag="PKH60_02850"
     CDS             6064..6960
                     /locus_tag="PKH60_02850"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNQ17019.1"
                     /translation="MFVISGSSNPQLAAEIAQLMGLESVPVEIGVFPNGEKRIHIQKD
                     VHGQNIVLVQSFSSPADEYIMEFLLLTDALERMGARHINAVIPWMGYSLQDKVFTNGQ
                     PLSAKVVADLVSQSYIKRVLLLDLHNQSIPGFYSVPTRQLSALELFAEHIAKTFEHQK
                     LVIASPDFGGLKRAREFAKKLDLELVNIDKQRDLRTGQVTASSVHGNVSNKIVVLFDD
                     CIVGGGTVVETAKLLKEQGAESVHFLATHGIFVNESQNKIQQSEVDSVLVTNSIQHPT
                     LVPKITELSVATLFADSLKQWL"
     gene            6968..8647
                     /locus_tag="PKH60_02855"
     CDS             6968..8647
                     /locus_tag="PKH60_02855"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNQ17020.1"
                     /translation="MRYSQLFGKSNKSPVKDSKFVSHQLLVKGGFIAESSAGRYFFLP
                     LGWRVHDKIKSVIKKHMDLTGAQEMISPTLHPLELWKETNRTNTVGFELMTVKDRRGA
                     AFALGGTAEEMFVDVVRKFQISYKDLPFTLYQFSNKFRDEMRARGGLLRVREFIMKDA
                     YSFDTDSEAFKHTYELYRNTYSAIFSELGLNTHIVEADNGYIGGEFCHEYQVEHSEGE
                     GTFFVSTDGSYCAHEDVATFIKEPKNTDESLKPYEEMAAQRGPTMEDGVNFHQLPAWQ
                     QLKDVMMVNEKNQLILAVIRGDLDVNEIKLAKATGSITLRSATTEEIRQIGSEPGFIS
                     PVGLDEKVMIVGDTSLRTFRNAYGGANKLNLDAHNINLDRDFSLDIEADIAMAKPDLI
                     APNGSGPLQMHRGIEVGNIFQLGTHYTTKMSHASYVDQEGTEKPYYMGCYGIGLGRTL
                     AAVVESHHDEKGIIWPESIAPFRFHLISLPGGETVAQAIYDQLRNSGQEVLWDDRSVS
                     AGEKFADADLLGCPYRLLCSTKTGEQVEFKKRGKSETKLMAVMELAQYYSK"
     gene            8913..9065
                     /locus_tag="PKH60_02860"
     CDS             8913..9065
                     /locus_tag="PKH60_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17021.1"
                     /translation="MIQSTEFDVNHDFEAIRHSLRDQVSLACDHAELFNSFEEFMESF
                     YLVQQK"
     gene            9182..9532
                     /locus_tag="PKH60_02865"
     CDS             9182..9532
                     /locus_tag="PKH60_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17022.1"
                     /translation="MIKAHWDLTDEELDSEEYRQEYADYYRDNIEKHTSHEFQHWIGL
                     QEHTSLNCRFQITFYRTSDNGLGFIPSISLNGKVEAETFVEKVLKAPDSPSLGDSKIL
                     IKLNAAIAKAKEEE"
     gene            9561..10439
                     /locus_tag="PKH60_02870"
     CDS             9561..10439
                     /locus_tag="PKH60_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17023.1"
                     /translation="METKKLLVTHHAPDLDAVGAVWLFKRFSPELFADAKVAFVNPGE
                     TITRTAAEILGFDLQDVTHVDTGLGEFDHHQPDRAVQPICAASLVFDDLSQSFPHLQN
                     DAALRTLVEYINDVDHFGEIHWPDSSHWRYSLMIHELLHGLEFQNPYDDEALLQFGMQ
                     CLDAAYSSLKQNLKAYEVIAHNGVEFELSAGIGLALETSNDDTIKIAQKQGYILVVRK
                     DPKLGNIRIKARPDSSIDLRKMYDLLCNKDPNSTWYYHNSGKMLLNGSHKHRNQIATK
                     LSLEEVVRMLRDCYGK"
     gene            10429..10911
                     /locus_tag="PKH60_02875"
     CDS             10429..10911
                     /locus_tag="PKH60_02875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNQ17024.1"
                     /translation="MENRGDCIFCQMQAGLTPYHQIWEDSDHFAFLSIFPNTLGFTVV
                     ATKNHFSSYAFEQTDAILAGLILATKRVAQILDSYFEDVSRCGMFFEGYGVDHLHSKL
                     FPMHGTGNGLDAWKIQETTFNTTYYEQYPGFLSSNNADRAEDAQLASLASAIRKSVSK
                     "
     gene            10927..11178
                     /locus_tag="PKH60_02880"
     CDS             10927..11178
                     /locus_tag="PKH60_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNQ17025.1"
                     /translation="MPIVITATQKDNTHDLIKQFKRATALADIVQVAKDRRYFQKPAK
                     VRADKRIQVKRLQKRLRSLKRTKNIPAAVISRLSEYLQS"
     gene            complement(11181..11678)
                     /locus_tag="PKH60_02885"
     CDS             complement(11181..11678)
                     /locus_tag="PKH60_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17026.1"
                     /translation="MTTNDQTLTGQQLAQQHLAAQTDEATDIDDAAEVAQSDKLAETL
                     QYLQNLIERNATQLKTVRDELREKRESLQNILENDQELTEAETVAKAQVDAVKQRKAT
                     LQSSPESINLKNEVGELREKLHEIEDTLNSHLLNYYQLTNSTSFDTSDGDQWDFTVKA
                     KLKSR"
     gene            11812..13236
                     /gene="pyk"
                     /locus_tag="PKH60_02890"
     CDS             11812..13236
                     /gene="pyk"
                     /locus_tag="PKH60_02890"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNQ17027.1"
                     /translation="MSKATKIVATIGPATESEEIIEELIRAGMNVARFNTKHGTPEWH
                     SERIVRVKQVAKRMGVTIGVLLDLQGPEIRITVPGGAAFPVQEGNEVLFVADGSGTDP
                     HQPQVPSNVIDTLNVGNHVLIDDGLGEFEIIGKENGQLKLKALGSFPVSNRKTLNTPG
                     VVIDQPSLIAQDFVHLDASNDELIDYVGLSFVRNRHDIEILREELAKRNLHANVIAKI
                     ENQSALDNLEEIIQASDVVMVARGDLAVEVPFKELTYWQKHIINRCRQFAIPVITATQ
                     MLKSMVDNPRPTRAEVSDVAHAIYDGTDAVMLSEETTLGKFPVEAVATQAVIAEFNEP
                     HTSLQTLETEYANSGALLTHAAINFLVDTNKSAEGQKVDKIVCLTETGATARYVARFR
                     PQVPVYALTGNISTCQKLSVVYGIYSIVINLPQDDRLESSEALITTMKSLDIAHPGDN
                     ILLIHGTFWKKPGLTNTLSFITIP"
     gene            13247..13534
                     /locus_tag="PKH60_02895"
     CDS             13247..13534
                     /locus_tag="PKH60_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17028.1"
                     /translation="MGKKVGLFSFVAGLAAGAAALFLSDDKNREKAEAELEKVAVKAK
                     QLKKDFDKDPEGTVRAVAKNVEGKAKKIVRTVKRTKVVVKTAPAKRKAVKK"
     gene            13674..13746
                     /locus_tag="PKH60_02900"
     tRNA            13674..13746
                     /locus_tag="PKH60_02900"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13707..13709,aa:Arg,seq:tct)
     gene            13778..13851
                     /locus_tag="PKH60_02905"
     tRNA            13778..13851
                     /locus_tag="PKH60_02905"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13812..13814,aa:His,seq:gtg)
     gene            13955..>14171
                     /locus_tag="PKH60_02910"
     CDS             13955..>14171
                     /locus_tag="PKH60_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17029.1"
                     /translation="MEIISRLKRLRKLPSSNDESKLQFLTENLDLFNNLSSVQSQFIL
                     ATIDAELETSKSFGLTLTDEEQYREKFA"
BASE COUNT         4474 a         2873 c         2918 g         3906 t
ORIGIN      
        1 gcatatatca gaaacaacac tcaactcgtc aaacaggcga gcatcaataa acaattgaat
       61 cccgctattg ttgatgagtt attaactgtc gatgaacaac gtcgcgccct ccaacaaaaa
      121 gtggatgatt tacgccgaga cagcaatgaa catgcgagaa agctcaaaca gaatgtagcc
      181 aatggaacac ttccaactga tgaagataaa aatctgggca aagaatttaa aaataaatta
      241 aaagaactcg agcctcaact gaaagaagtt gaagacaagt atcaagcgat gctgtatcaa
      301 attcccaatg taccggcgaa cgatgtgcct gtaggacctg atgaatctgg caaccaaatg
      361 attcgtcaag ttggaaaacc aaaaacattt gacttcgatg tcaagccaca tgaagaaatt
      421 atgacaaaac taggttggct ggatgttgat agagctgtaa gaattggcgg atttcgctcg
      481 tatttcttaa aaggagatgg tctccgcctt gaacaagctc tcttgagagt cgcgctcgat
      541 agattagtca ctgctggttt tgaacccatg tcggttcctg tgctcgtaaa tgaggatgct
      601 atgtggggaa ctggttactt cccctggggg caagaggatc actataaaac acaagataat
      661 caatttttga ccggcactgc cgaagtcgct ctgactgcgt acagacaggg cgaaacttta
      721 acggaaaaag agttgccttt tcaaatctgc ggcatttctc cttgcttccg ccgagaagtt
      781 ggttcatacg gcaaggacac ctcgggtgtg attagagtgc atcaatttaa taaagttgag
      841 caggtagtct acaccgtggc ggatgaaaca attactcgag aatggcatga aaaaatggtg
      901 aggttggctg aacagctact ggaagatcta gaattaccat accaggtcct tctgatgtgc
      961 accggcgaca tgggagcggg tcaacgaaaa aaatacgaca ttgagacatg gtttcccagc
     1021 caacaaaaat accgggaaac tcattccgct agctacttta atgattttca gtcacgtcga
     1081 ctcaatatta aataccaagc caaggatggt tcaactaagt ttgtctacac gctcaataac
     1141 actatggcgg cgtccccaag actgttggct gcgatcatcg aaaactatca acagccagat
     1201 ggctcggtcg caatacctaa attattgacg ttttaagatc gacaaacagc tattcttttg
     1261 gcataatcag gatcttatgt ttaagtttct cacctcaatt tttgatgata ataaaaaagc
     1321 gatcaatcga ttttctgctc aagttgatca aattaaccga cttgaacctg aaattaaaaa
     1381 gctctccgat aaagctttgg ctcaacaaac taaaaaattt caggatcaaa tagaaactga
     1441 actaaaatca gggaaatcag aaaaggcagt gctcaattca atcttgcccg aagtctacgc
     1501 cactgtgcgt gaagtatcac gccgagtcat cggtttacga cattatgatg ttcagttgat
     1561 tgctggaatc gccttgcacg aaggtacgat tacagagcag aaaactggag aaggtaaaac
     1621 cttaaccgtc acattaccgc tctatctcaa tgctttactg ggtaagggcg ctcatctcgt
     1681 caccgttaat gattacttag ccgaaactgg agccgggtgg atgggaccaa tttatcactt
     1741 cttaggctta accacctccg tcatcactca tgaaaaagcc agcctctatg accccgattt
     1801 tactggagaa gataggggag atgagcgcct cgaacacttt aaacccatat ctcgcacaga
     1861 agcctatgcc gctgatatta cctatggaac taacaatgag tttggttttg attatctgcg
     1921 tgacaacatg gtgcaatcac tcagtcaaat ggtacaacga ccaaacaata ctcattttta
     1981 cgccatagtt gatgaggctg actccatttt aattgatgag gcacgcacgc cgctgataat
     2041 ctccgcccca gattccgaac cgaccaaaaa atattatgat tttgcccaca tggtaacttc
     2101 gctctcaaag ggagcagatt atacggtaga tgaaaaagcg cgcgcagtta cacttaccga
     2161 cttgggagtc aagcgcttag aacaaaaact aaatgtgaag aatctctacg aagaaaactt
     2221 tgacaccatt caccatgttg aatcggcgct caaggccaat accattttcc aaagagataa
     2281 agactatatt gtgcgtgata atcaggtaat tatagttgac gagcatactg gtcgcctcat
     2341 gtacggcagg cgttattccg atggacttca tcaggcgatc gaagccaaag aaaatgtccc
     2401 gattcaacaa gaatctcgaa ccctagcaac aatctctatt caaaactact tcagactcta
     2461 cagtaaactt tcgggtatga ctggtactgc cacaactgag gctgaggagt ttagaaaaat
     2521 ctatgccatc gatgtgatag ctgtaccaac taatgacacc gtcatacgtg tagataaaac
     2581 tgatgtagtt tataaaactg ctggagcaaa atatacagcg attgccaatg aaatcgagac
     2641 cattcacaaa gagggcagac ctatattagt tggtactcgc tccatcaatc ataatcaaat
     2701 tgtggccgat ttcttatcta gaaaaaaaat tacccaccaa gttttgaacg caaaaaatca
     2761 cgagaaagaa gcatttatca ttgccgatgc tggtaaaaaa ggcgccataa ccgtagcaac
     2821 caacatcgct ggtcgtggag tagatattgt gctgggtggt gcaaaaccag agttgaaaga
     2881 ctatcgtaaa aaaactgaat caaaagttaa agccactgca gatcaaataa aattggccaa
     2941 aactctgaat ctaccaccat cgatcgatcc aactcaatat aaactcaaag aatatcaaca
     3001 ggccatggaa aaatggcaat cggaccacga tgaggttgtg agattgggtg gcttgtatat
     3061 tctgggtacc gagagacacg aatctcgtcg catcgataat caactacgtg gccgagctgg
     3121 tcgtcaggga gatccgggta cttcacaatt ttttatctca cttgatgatg aaattatgcg
     3181 gatctttggc ggtgaacaaa tttctaaaat catggacatg ctcaaaattg acgagggtca
     3241 gccaattgag cacggcatga tcagtaaatc tattgagtct gctcaagtaa aggtcgaggg
     3301 gtttttcttt gatcaacgta agcgcttagt agaattcgat gacgtgatga ataaacagcg
     3361 cgaaattacc tacattcgca gacgccgttt gctagaagaa ggttcagtct tagaaaaagg
     3421 cgaaaaaata accgcagagc aagaagaggt agtcgccggg cttcgtcaac aaattcaggg
     3481 atatgtagac gaagaaattc gtgctttagt tagcttgaat gcgcccaaag aatttactgc
     3541 agaagaatat gccaccatag taaaagaatt ttgccgatta gtaccattcg atcaagcttc
     3601 gcagttgcga ctcgaaaaag aaatttcaaa aattaccgat ccagaagaaa taacagaaaa
     3661 attacaaaca atttcacaac aactgtatga ccagcgagaa aaagatttag gcgagatgac
     3721 catgcgtcaa cttgagctct acgtcaccct cagtacgctg gatgaaaagt ggatggacca
     3781 cattgatgcg attgatagtc tcagagaagg tatctggtta cgaggtgaca aacaaaccgt
     3841 gctttctgaa tataaaaaag aggcatttgg tatgttccaa caaatgctgg gcgccgttga
     3901 ttcaaccatt gccaaccgaa ttttccgcct acaaccaatt agcagagaac aattccgagc
     3961 aactcaacaa aaacaattaa tagaacaaaa ggatgatatt aacgaggaat tgagtaaaga
     4021 agtggctgac gctactatcc caagctcagc ccccacacaa attaagggcg acactcaaga
     4081 tttagccgca gccctgcgtt ctgccaaagc agttgccaaa ccacagcctg ggtctaaaca
     4141 aatgaaaata ggccgtaacg acccctgtcc ttgtggatca ggactaaaat acaaaaagtg
     4201 tggcctagtg ggtgctccag agcatcgtag ataaacatat caccatggaa attgatcaga
     4261 gatattctat tcaccaattc gtaattgatg tggctcggta tatccgtccc tatcgcacgc
     4321 agttcttgat tggaatattc cttcgcataa caagcgacgc gtctcgacta tatcccgcct
     4381 gggcactcag taggataatt gaattactga ctaactctgg aactaatacc gatcccgaag
     4441 agctcttgag aattttcgta ctgtggggac tggcgtatgc atactttggt ttaactcata
     4501 atctatcaaa attttttggc taccaagtag cagaaaaggc ttctttagac atttataaag
     4561 aaactcttgc ccatattttt accttagatt tttcttggca agaaaaagaa aacagcggaa
     4621 ataaaatgaa gcgaatcgac aagggtcttg acggaattaa cattaccatt cgaaggatat
     4681 tcgacacatt gctctcagtt ttggtaaata cggttggcat agtatttatt ttctttggct
     4741 tagatccaac cctaagtgtt ttactggttt tgtttatgat tacgtattac gtatttagca
     4801 ctttgttgct taaaagatct ataagacaag aaaaaatagt caataaagca tttgagaatt
     4861 tgagcggcat cacatttgaa gctttgaata atatccaaac aattaaggcc ttatctatag
     4921 attggggcgt caatgagtct gtaaactccc atattaaaaa gttaatacaa aatattaaaa
     4981 aacgaatttt ttactatcaa tctcaaggag ctttgcaggt tatctaccaa tctgttttct
     5041 ttttaagcat agtagcttgg ctttcttggg acatttacca tggaactaag aacgttggac
     5101 tactggtatt gttttttggc ctgtttaaca aagtgagtga ggcaacagag gaattgacta
     5161 atgtcacgca acagcttgct ttggcaaaaa tatggatctc tcgcgcaatg aacatcttaa
     5221 agaccaagcc agaaattgaa gaccctaaaa aagtttcgct acaacaaacc tatccaccag
     5281 actggaaaac aatcagaatt gaaaaactcg gatttggcta tggtaaaaaa agcgctttga
     5341 acgaaatatc tctaacgatt agacgaggag aaaaaatcgg aatagttggt ctgtctggag
     5401 ctggtaagtc tactttattt aaattactcc tcgatctata cgaagactat gatggtcata
     5461 ttctcattga caatattcca cttaaaaacc ttcaaaggca gtcatatatt gatcacgtag
     5521 ctgtagtatt gcaagatact gaattatttg acatgagctt aaaagataat atccaaattg
     5581 cgggagttcc tggcgcttcg cgacaaatta atttgacaga agttatcaaa atggcccatc
     5641 tggaaagtgt agttgatcag cttgagaatg gagtagattc aattgtgggt gaaaaaggga
     5701 ttaaactgtc tggtggccaa agacaacgag tgggtattgc tcgcgcttta taccgtcagc
     5761 cagatatttt gctgcttgat gaagccacct cacaccttga cgctcattcc gaaaaggaaa
     5821 ttcaacaagc cctgcaagaa aacttacata aatttactac gattgtgatt gctcatcgtt
     5881 tatccactat tattaaaatg gacagaatcg tggttctcag ttatggcaaa atcgtggagc
     5941 agggcagttt tcaagaatta ctcaataacc aaggtgcttt ttctaagatg tggaaagagc
     6001 aaaaaattta atttcattgt ctcggagtgc ttttgtcata cgatatgcga tataatgagt
     6061 gatatgtttg tcatatctgg atcatccaac ccccaattag ccgctgaaat tgcccaattg
     6121 atgggtcttg agtcagtacc tgtagagatt ggcgtttttc ccaacgggga aaaacgcatt
     6181 cacatacaaa aagatgtcca tggtcaaaac atcgtcctcg tgcagtcttt ttcatcgccc
     6241 gctgatgagt acattatgga atttttacta cttacagatg ccctcgaacg aatgggagct
     6301 cgccacatca acgccgtcat cccctggatg gggtattcac tacaagacaa ggtctttact
     6361 aacggtcaac cactttctgc caaggttgtc gcagatttgg tgtcacaaag ttatatcaaa
     6421 cgcgtccttt tactagacct acacaaccag agtattcctg gtttttattc tgtacctacc
     6481 agacaattat ctgctttaga attatttgcc gaacacattg caaaaacatt tgaacatcaa
     6541 aaactggtga ttgctagtcc tgactttggt ggacttaaac gcgctcgaga atttgccaaa
     6601 aaactcgatt tggaattggt aaatatcgat aaacaacgcg atctgcgtac tggtcaagtt
     6661 acagcttcaa gcgtacatgg aaatgtatcc aataaaattg tagttctttt tgatgactgc
     6721 attgtaggtg gtggtactgt ggtagaaacc gcaaaattgc tcaaagaaca aggtgcagaa
     6781 agcgtgcact tcctggctac acatggaatt ttcgttaatg aaagtcaaaa taaaattcag
     6841 caatctgagg ttgatagcgt tctggtcact aactcaattc aacatcccac tctagtacca
     6901 aaaattactg aattatccgt tgctacacta tttgctgatt cactcaaaca gtggttataa
     6961 agtttttatg cgctattctc aactctttgg aaaatccaac aagtcaccag ttaaagattc
     7021 taaatttgtg agccatcaat tgttagttaa aggtgggttt atcgcagaaa gcagtgctgg
     7081 tcgctatttc tttttgccac tgggttggcg cgtgcatgac aagatcaaat ctgtcataaa
     7141 aaaacacatg gatctgactg gagctcaaga aatgatctct ccaactctcc accccctgga
     7201 attgtggaaa gaaactaatc ggactaatac agttggattc gaattaatga ctgtcaagga
     7261 tagaagagga gccgcatttg cgcttggagg cactgctgaa gaaatgtttg ttgatgtggt
     7321 acgaaaattt caaatttcct ataaagattt accgtttaca ctctatcaat tttcaaataa
     7381 atttagagac gaaatgcgag ctagaggggg actgcttaga gtaagagaat ttattatgaa
     7441 ggacgcttat tctttcgata ctgattctga agcttttaaa cacacttatg aactctatcg
     7501 taatacctac tcagcaattt tttctgaact gggattaaat acacacatag ttgaagcaga
     7561 caatggctac attggcggcg aattctgtca tgaatatcaa gttgaacatt ctgagggcga
     7621 gggaactttt tttgtttcaa ccgacggttc atattgtgca cacgaagacg ttgctacctt
     7681 cattaaagaa ccaaaaaata ctgacgaatc attaaaacca tatgaagaga tggctgcaca
     7741 acgagggccc accatggaag acggggttaa tttccaccag ctaccagctt ggcaacagct
     7801 caaagacgtt atgatggtaa atgaaaagaa tcaattaatt ttagctgtca ttcgtggaga
     7861 tctcgatgtg aacgaaatca aattggccaa agcgacaggc tcgatcacgc tcagatctgc
     7921 aacaacagaa gaaatcagac aaataggatc agagccagga tttatttctc ccgttggctt
     7981 agacgaaaaa gttatgatcg tgggagacac ctcgctgcgc actttccgta atgcgtatgg
     8041 tggggctaac aaattgaatc tagacgctca taatatcaat cttgaccgcg atttttcact
     8101 agatatagag gctgacattg ctatggccaa acctgatttg atcgctccca atgggtctgg
     8161 acccttacaa atgcatcgag gtatcgaagt gggcaatatt tttcaacttg gcacacacta
     8221 cacaactaaa atgagtcacg caagctacgt cgatcaagag ggcacagaga aaccgtatta
     8281 catgggttgt tatggtattg gacttggcag gactttggct gcagtcgtag aatcccacca
     8341 cgatgaaaag ggcataatct ggccagaaag tattgcgccg tttcgttttc acttgatttc
     8401 tttacctgga ggagaaaccg tagctcaagc aatctatgat cagctcagaa atagtggaca
     8461 ggaagtattg tgggatgatc gctcagtgtc tgcgggagaa aaatttgccg atgccgattt
     8521 gcttggatgt ccttacagat tgctctgttc aaccaaaact ggtgaacaag ttgagttcaa
     8581 gaaacgtggc aaatctgaaa ccaagctaat ggctgtgatg gaattggctc aatattactc
     8641 aaagtagtcg ttattgatgc aatttctact gtaattattt aggcttatag cagaattctt
     8701 tatattgagt agcagaaatt atagactgtt ttatacgtct tttttcttac tagttttacc
     8761 caccgcattt cgtatactac acagcacaat cccggtgagg gtgcccgcgg cgatgtttcc
     8821 ggcttagtac gtaaaaaata ctatatttag gaacattgcg gcgggttttt ctttatatat
     8881 atcaaaatat atcctagtag tataattgtg tgatgattca atctactgag tttgatgtta
     8941 accatgattt tgaagccata cgccattccc tacgagatca agtaagtctt gcctgcgatc
     9001 atgccgaact tttcaattca tttgaagaat tcatggaatc tttttatcta gtccaacaga
     9061 aatagaaatt gatgaaaata aaactccctg gatagtcctt gagtggaaaa atagaccgtg
     9121 ctcagtgtat gttgatcaaa gccatctcta tccaacatta ctactcctta gttgtcgcca
     9181 aatgattaaa gcacattggg atttgacgga tgaagaatta gattctgaag aataccgaca
     9241 agaatatgcg gattactatc gagataacat tgaaaaacac acttctcatg aattccaaca
     9301 ttggatcgga cttcaagaac acacctctct aaactgtcgg tttcagatca cattttacag
     9361 gacgtctgat aatggtcttg gttttatccc ctccatttca ctcaatggta aagtagaggc
     9421 tgaaaccttt gtagaaaaag tattgaaagc gcccgactca ccatcgcttg gagattctaa
     9481 aatactcatc aaactgaatg cggcaatagc aaaagcaaag gaagaagaat aagtgccctt
     9541 ttcagtgtat actcttgagt atggaaacaa aaaaactcct ggtaacacac catgcgccag
     9601 atcttgacgc agttggagca gtctggctct ttaagcgatt ttcgcccgaa ctctttgcag
     9661 atgcgaaagt agcttttgta aatcctggtg agacaatcac tagaacagct gctgaaattc
     9721 tcggttttga tctgcaagat gtgacacatg ttgatactgg acttggcgag tttgatcacc
     9781 atcaaccaga tcgagcggta caacctattt gtgcagcatc gctcgtcttc gatgatctga
     9841 gtcaatcctt tccccacttg cagaatgatg ctgccttgcg caccctagtt gaatacatca
     9901 acgacgtcga ccattttggt gaaattcatt ggcccgattc ctcccactgg cgttatagcc
     9961 tgatgattca tgagttgctc catgggttag aatttcaaaa tccgtatgat gatgaagcgt
    10021 tactccaatt tggaatgcag tgtcttgacg cggcatactc atccttaaaa caaaacctaa
    10081 aagcgtatga agtaatcgca cataatggtg ttgagtttga actcagcgca ggtatcggtc
    10141 tagctctaga aacaagtaat gacgacacga taaaaattgc tcaaaaacaa ggatacatct
    10201 tggtagttcg caaagatcca aagttgggca atattagaat taaagctaga ccagatagct
    10261 ccatagattt aaggaaaatg tacgatctat tgtgtaataa agatccaaat agcacttggt
    10321 actaccacaa tagtggcaag atgctgctca atggctcaca taaacaccga aatcaaattg
    10381 ccacaaagct cagtctagaa gaagtagtgc gaatgctgag agattgctat ggaaaataga
    10441 ggtgattgca tattttgcca aatgcaggca ggtttaactc catatcatca gatatgggaa
    10501 gatagtgatc attttgcgtt tttatctatt tttccaaata ccttggggtt caccgtagtt
    10561 gctacaaaaa atcattttag cagttatgcc tttgagcaaa cagatgccat tttagctgga
    10621 ttaatcctag caacgaaacg agtcgcccaa attcttgatt catattttga agacgtcagt
    10681 cgatgtggca tgttttttga gggctatgga gtggaccatc tgcatagtaa attatttccc
    10741 atgcatggta cgggcaatgg tttggatgcc tggaaaattc aagaaaccac attcaatacc
    10801 acttattacg aacaataccc aggatttctc agttccaaca acgctgatag ggcagaagat
    10861 gcccaacttg cgtctttagc ctcggctatt cgcaaatccg taagtaaatg atataatccc
    10921 tcgtctatgc cgatagttat taccgccaca caaaaagata atactcacga cttgatcaaa
    10981 caattcaagc gagcaactgc gcttgcagat atagtccaag ttgccaaaga taggcgctat
    11041 tttcaaaaac cggcaaaagt tcgtgctgac aagcgaatcc aggtcaagag attacaaaaa
    11101 cgattgcgat cattaaaacg cacaaaaaat atccctgcag cggttatctc ccgactctcg
    11161 gaatatttac aaagttagtt ttatctgctt ttcagtttag cttttaccgt gaaatcccat
    11221 tgatctccat cgctggtatc gaaactggtg gaatttgtga gttggtaata attgagaagg
    11281 tgactattca aggtgtcttc aatttcgtgt agcttctctc ttaattcacc cacttcattt
    11341 ttcagattga tggattctgg tgatgattga agcgtagctt ttctttgctt tactgcatca
    11401 acttgtgctt ttgccactgt ttctgcctcg gttaattcct gatcgttctc taaaatattt
    11461 tgtaagcttt cgcgtttttc tctcaattca tcacgaactg ttttgagttg agtcgcattt
    11521 ctttcaatca aattttgtaa gtattggagc gtttccgcca acttatctga ctgagctact
    11581 tctgccgcat cgtcaatgtc tgttgcctcg tctgtctgag ccgccaggtg ctgctgcgcc
    11641 aattgttgtc cggttagtgt ttgatcattc gttgtcatta ttacctttct gactatatac
    11701 aataaaaata cattttgtga tcactgaata tacacgtata atgattttta tccagttaaa
    11761 ctgattgaag cttactatag cttattcaat tcgacaagtc agaaaggcaa gatgtccaag
    11821 gcaaccaaaa tcgtagccac catcggtcca gccaccgaat cagaagaaat aattgaggaa
    11881 ctaattcgtg ctggtatgaa tgtagctcga tttaacacca aacatggtac ccccgaatgg
    11941 cattcagaaa gaatagtgag ggtcaaacag gtagcaaaac gaatgggtgt cacaattggt
    12001 gttttgcttg atctacaagg tccagaaatt agaataactg ttccaggagg agctgcgttt
    12061 cccgttcaag agggtaatga ggtgctgttt gttgccgacg gctctggcac tgatccacac
    12121 caaccacaag ttcccagcaa tgtgattgat acattaaacg tcggcaatca tgttcttatt
    12181 gatgacggct taggtgaatt tgaaataatt ggtaaagaaa atggacaact caaattaaaa
    12241 gcacttggtt catttccagt atctaatcgc aagacactca atacacctgg agtggttatc
    12301 gatcaacctt cactaattgc tcaagatttc gtccatcttg atgcgtcaaa tgacgaatta
    12361 attgattatg ttggactgtc atttgttaga aatcgacacg acatagaaat tcttagagaa
    12421 gaattggcca aaagaaattt acatgcaaac gttattgcaa aaattgaaaa tcaatcggct
    12481 ttagataacc tagaagaaat tattcaagcc tcagatgttg tgatggtagc tcgaggagat
    12541 ttggcggttg aagtaccgtt taaggaactg acatattggc aaaaacatat tatcaaccgt
    12601 tgtcgtcagt ttgccattcc agttatcact gctacacaaa tgctgaaatc gatggttgat
    12661 aaccctcgac caacccgcgc cgaagtctcc gacgtagctc acgcaattta tgatggcact
    12721 gacgcagtca tgctttctga agaaacaacc ttgggaaaat tcccggtcga agcagtagca
    12781 actcaagcag tcatcgctga atttaacgaa ccacatacat cacttcaaac tttggaaaca
    12841 gaatatgcaa attcaggcgc cttgctgact catgccgcaa ttaatttttt agtcgataca
    12901 aataaatctg ccgaaggtca aaaagtagat aaaattgttt gtctgactga aactggcgct
    12961 accgcccgct atgtagctcg atttagacca caggtaccag tttatgccct cactggaaac
    13021 atttcaacct gtcagaaact aagcgtagtc tatggtatat actctatcgt aattaattta
    13081 cctcaagatg accgcctaga gagttcagag gctcttatta ccactatgaa atcgctcgat
    13141 attgctcacc ctggtgataa cattttactc atccatggca ctttttggaa aaaaccaggc
    13201 ttaacaaata cgttatcatt tattacaata ccgtagagga ggatatatgg gaaaaaaagt
    13261 aggattattt tcttttgtag ccggtttagc cgcgggggca gcagccttgt ttctttctga
    13321 tgataaaaac cgagaaaaag cggaggctga actagaaaaa gttgctgtca aagctaaaca
    13381 attaaaaaaa gatttcgaca aagatcctga aggtacagtt cgcgcggtag caaaaaatgt
    13441 tgaaggtaaa gctaaaaaaa ttgttcgtac agtgaaacgc accaaagtag tggtaaaaac
    13501 cgctcccgca aaacgcaaag ctgtaaaaaa gtaatttttg ctcgtttctt ctcatctggc
    13561 caaagctagg gtagaataca gttgttgcgc ctatagctca cccgcctgca acgctttgcg
    13621 tagcattgcg ggcggggtgg atagagcaat ggtaagatat gcaactgtca aatgcgccta
    13681 tagctcagtg gatagagcaa ctgccttcta agcagtaggt cgctggttcg aatccagctg
    13741 ggcgcacctg ttctgttaca atactgatct gtttatggtg gctgtagctc agctggttag
    13801 agcgccgctc tgtgaaagcg gaggccgggg gttcaagtct cctcagccac ccaatcaata
    13861 atagattttt ctcctgatta tttacgccat tgtagtactc aagaataaat aacttttatt
    13921 gatacctatt aaacctgata aaatcattac caacgtggaa attatttcta gattaaaacg
    13981 gttacggaaa ctacctagta gtaatgatga gtcaaagcta caatttctga ctgagaattt
    14041 agacctgttc aacaatttga gtagtgttca atctcaattt attctagcta ctatagacgc
    14101 agaattagaa actagtaaat catttgggtt aacactcaca gatgaagaac agtatagaga
    14161 aaaatttgct t
//
LOCUS       DAOQZP010000028        13092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_5254_length_13092_cov_5.206719, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000028 DAOQZP010000000
VERSION     DAOQZP010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 13092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13092
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5254_length_13092_cov_5.206719"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2501
                     /gene="lon"
                     /locus_tag="PKH60_02915"
     CDS             <1..2501
                     /gene="lon"
                     /locus_tag="PKH60_02915"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNQ17030.1"
                     /translation="SLAFRMLIVTEETKKPTKLPQVEAIFSGENNSNQAEVIKTTKSP
                     EIRPPERFILPAVPIREGVLFPSTESVLTFGRQISLSSINQVTPKQNSVVLITQKRPD
                     VEEPKADDMYTIGTLALVDKKIKTDESINALVRGIGRVKIIRFIQLSPKMLVEVEKLQ
                     DIEQKDEESAALTNHLQKEFRRAVHMGKPVEFLNFMKLMGGVTDGELTDQIASTLNVE
                     TSEKQSILETLVVKERMRMVIDKLAHEMKVLEIEKDVVNKTQKKFDKSMRENVLRERL
                     RTIQKELGEIDDEEEIVDEYEEKLKKITVDKDIKTKISKEIKRLKQMSPNNPEAGYVR
                     TWLDTIFELPFGKYSKTKVKIQQAEESLEKSHYGLEKVKDRILEYIAVLELKQRTAKK
                     EGDRRLPTILCFVGPPGVGKTSIGKSIAESLKRKFVKISLGGIRDEAEIRGHRRTYVG
                     AMPGRIITGMKQAGSMNPVFMLDEIDKIGNDFRGDPSAALLEALDPEQNSSFEDHYLD
                     MHFDLSEVMFITTANTLDTIPPALRDRLEIIRYSGYTEEEKFNIAKKHLFHKVRSANG
                     LTGNHMQITDESIRFIIQRYTSEAGVRDLERNLHKIMRKLARKLTESTKTTKTIVTPK
                     IVREFLGPEKFDVEMSSKTDVIGQATGLAWTSVGGDTLFIEVSLTPGKGAVQLTGKLG
                     DVMKESAQTAYTFVKANAEKFGIAPSRIAKTDIHVHVPEGAVPKDGPSAGVTITTAIV
                     SAFTNIAVKKDIAMTGEVTLRGRVLRIGGLKEKSLAADRAGISTIIIPKSNERDLEEI
                     PKTVLSKIKFIPVEQVEEVIALALVKKPKARNIQ"
     gene            2529..3398
                     /locus_tag="PKH60_02920"
     CDS             2529..3398
                     /locus_tag="PKH60_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNQ17031.1"
                     /translation="MRSFFRVIFTVVFILFTGLSFSACTQIFEKEALAGIQITTGQIE
                     SVVFINGQYLDKSPLVRKNLKPGEYIIEIKPANDALASYQTSVTLRQELLTVITWNPA
                     ATPEQSGGVVYEMEPLPMKDATEVSFSTVPDGAIIQLMDKPKEIAPYTFKNVSPGEQE
                     YSISLPSYEQQKHTINIVPGYRMLISLKLAKQSSILLMPTPTITPVSSASSSATASSS
                     GTVTILKTNLFVDGVEVLRVRDLPNVSGTEVGLAKVGVTYPYLNEKKDGWFKIKLASA
                     SGWISGTYANLKE"
     gene            complement(3382..4710)
                     /locus_tag="PKH60_02925"
     CDS             complement(3382..4710)
                     /locus_tag="PKH60_02925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNQ17032.1"
                     /translation="MNKVTIAHLVIILLLGVITRFAFLDSVPGNLHPDSVDTIRIYLE
                     HRFAGNFSLLSTNWNGSHIINQLMIAIPWEVMGMPYWAVQMGPALISIICLLAFYLLV
                     KSWLNNAVIALLSTLLLMVDPWFLNFSRSGWENIANCLGVILLLYSFTPHSKKHGFQK
                     ILMVLVAMGSPYLYHPGKIIAFTALIALIVVSLKEKIGLSKKIMQLLIILILLSLSIM
                     PMFFIDSVNQFGRITTVSIFSEIDVKEALLFNLKNNWLGFLTFQPEQWSIGINSRYVP
                     LDSWIIHPVVVLLFMVGIIYTVWKRWWLVGVGLLLIVPVNIFSNNTPDAARTIHALPY
                     IYFMGTLGAHSLYLLLKRFYNSKFCIKPVIRMIFLGSVVTGVLGLMSIQLLHYWNWIT
                     DPKTLVVREPAVYSHEYDRWLNDTFAQLKNSGRTLSIYEWRDVQTTTLSN"
     gene            complement(4703..6658)
                     /locus_tag="PKH60_02930"
     CDS             complement(4703..6658)
                     /locus_tag="PKH60_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17033.1"
                     /translation="MTKLYQLFVVVIIIILALARVIAIDKIPPATFIDEFNLSVPAAQ
                     IALGNISIPWNGFGWYATPGLFFYYVAFLFRLFGISISIIKLAWIIPAIVSLPLVYQL
                     GKKLFSSKIVGLASLILYGLNVTSVHIARWGHGAVITAMFQLLSLLFLWMSVESKNWK
                     SNLYIMGAACVMGLSLYFYVGARSYVLLFPLYIFWYWVYSKRKLQKKIIGSLSFIAVI
                     FLVAFPIVLEMQKDPFNFIWRIKEVSIIQSDQSLRSNMDALSKNSIIYLRIFYDAIDE
                     NLRHNPLQSTLFPYYLGLLIPASIVILCIKKSTRKQGIFLLLGLIATMTGGVLSEEAP
                     SYFRIFGSIPIMAICIAVIIFKIPFSSCLIKKSQINLSYLKPGLLLLFSSSVALLLAR
                     YYLFMLQPNFSLLQAFSYPEQIIAQTANKYRELGNQVFLSTDYLYSSTIFLTLPKQAN
                     LEFRMFDIDALLEIDEPATLILESSAVGLQSYLEEYFTIVNMFELETGQETRPIVYEL
                     LPKRPPSELVGLSVTCDNGQMQLLQKQSLGIYHSLQTFPENIVNCNWKGKIAITQSGE
                     YSLKGSADDSVDMQLASSSGEIIFSQQPEKNWVQTVILESGIYELVVSYQNLGGARNV
                     RIEWLKPGSTIYEVIDPLLLTKNQTNE"
     gene            6698..8455
                     /locus_tag="PKH60_02935"
     CDS             6698..8455
                     /locus_tag="PKH60_02935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNQ17034.1"
                     /translation="MKQIIYRLFFIGIILVLVGSRFFGNNYDNLRHLHPDERWLVMVV
                     GRLHFFDNLNPDFFAYGSLPLYVLKAFAQLSDATFQTQYDNYDGLLILGRSLVSIIDI
                     ATAIVVFLITRKITKNTLSALFSMLGYALLFFPIQNSNFFIVDNFVNLFFSATLLFLI
                     RYVEKPNWQRLTILSVIYGLLLASKITPIILLAPIVFVLFSMPFANSILHNRLLLSIK
                     SKLIQLFSSLSSIVNHKTHHPRLLFSSLVKSLANVLLFFSITILTVFIGMPFTVINYE
                     QFIREISAQMTMNSNAYIFPYTLQYVDTTPYLYYLKQIAIWGAGPILSMIALVGLGLS
                     ITQLVKYWKNKGLLQAIFQPLTIYLFANLIYFIVIGRSAVKFMRYVLPLYPSIAVFIG
                     IALGFMFSSKKFPKLFRWTLISTILVLASLWTSAFVSIYSQPHTRETASLWMLQNIPP
                     GSRLAEEHWDDRLPLYSGELFEYIDLPLYELPDDANKWKLIANRLEQADYIVMTSNRL
                     YGSLPKLADCSNHEKCYPLTAQYYNDLFAENLNFVKVAEFTSRPTIFGIEIFDDNADE
                     SFTVYDHPKVIIFQKGSRR"
     gene            8452..10086
                     /locus_tag="PKH60_02940"
     CDS             8452..10086
                     /locus_tag="PKH60_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17035.1"
                     /translation="MKSRYCSLLISIIICIFTGCLLFYNLNNYPPTLYNWENYSVWNI
                     FQQRFNEPTISMASYFQRADGLMTESGFSPIVGLPMYLITEIFGYSLETLRIWPATLT
                     IIGVLVFFLFILKVFGKKISAIATILLITSQGFLLYGRTATNVAPTLLAESVTAALIY
                     YFLLKPKSFALFLSALVMVVINYFFYAPIRFFTPILLFCIARYLLLHVTHLLSNNDAV
                     IKSGEILKLLVIGVFVSFAIVIIYFSKSLFLNYFHARGEQIFASITQINPSVYHIKNP
                     TMQIFVNTKQYLSLLFSIDSKPVIIDYGNHYGQMMHRALVPFFILGFFVMINGLKKKN
                     RKYRLLAFWFLLTCVPILLTNNVHIGRLFLHLSPLFVVVAIGLSKLTEVIISRFNSVF
                     PNRLAVSKRASYTSLSLFLLIISVIEIRAYFLTPPTIDHNIKVLKENESEYISKTIYV
                     INTEASTLHFWEVAFYLREKFYFKDGMNLKPLDMARNQEQVGEGYLFRTQGSLDTLAS
                     ACDNSDSALIVVGRNSKFKSPLELTNCRASTLLLYQ"
     gene            complement(10092..11138)
                     /locus_tag="PKH60_02945"
     CDS             complement(10092..11138)
                     /locus_tag="PKH60_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNQ17036.1"
                     /translation="MKVLITGGAGFLGLHLARYYHVKGHHVRLLDIAPFPKEEYDSKM
                     ELISGDVRDAKIATAATKGMDVIIHAAAALPLWTAEEIVSTNVEGTRQMLAAAGRAKI
                     KRFIYISSTAVYGVPKKHPIVETDPRIGVGPYGISKIQAEDHCFAAIKTGQPVTIIRP
                     KTFVGTERLGVFEILFDWIHDGKRIPVIGNGENRYQLLDVDDLVEAIYRFSNVTKNTS
                     RFDDAFNIGAKKFQTVGKDLDQLFKKAGSGSSLFPTPATPIKMILRLLELLRLSPLYQ
                     WVYDTADKDSFVSIEKLEKVLQWSPRYSNAEALIKSFQWYQKHYDEVKSRKSGVTHTV
                     GWKQGALGIVKKLF"
     gene            complement(11135..11719)
                     /locus_tag="PKH60_02950"
     CDS             complement(11135..11719)
                     /locus_tag="PKH60_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17037.1"
                     /translation="MPQKITKKTLKVGFDFDGVVLYNPARVLRPFLGYLRGKKKQNGS
                     TQLVFYHPKRKLERWFWWLAHQSSLFPARGLQQVRQMVSENKIEAYVITGRSTFLKSD
                     FERRLKSIHARQFIKQSFISESDEQPHEFKAKMINSLKLDCYVEDNWNIAQYLATNTK
                     ARIFWISNVADFHFAYPDKYLSFEAVVEEIRKML"
     gene            complement(11704..>13092)
                     /locus_tag="PKH60_02955"
     CDS             complement(11704..>13092)
                     /locus_tag="PKH60_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17038.1"
                     /translation="KTLFRSAVAGILAVILFLFNGSLAWVEFFNQHGWTWEALTSIPQ
                     QSQFASFGPWSGRLVSAFWNWNIFTNQRHLGLSYGMFLLLLYPLVRLSVIPKESLKER
                     WSRSLTSLFGGVLGAVKKISSKKNSNQLFNRYWWQPLLVISFVIFPLLHQAAYIILVP
                     LTLIWIGITWPKSKSVLLVYGLAVLVSIIVFKVFTPQSGSLPIWQIGYLSPEKTLMAI
                     NWYWFNNLGLYWLLLPVLLIWAVIKKQWWIWTALPFFVIANLFRFSADMINNHKLITF
                     FIIALQITTAGFLVSLVKQHKVLLIIIVPLLVGLTFSGIIDVFPITNDYDGEISDWKK
                     SPMQQWIKNNTQPQAQFLTASYMYSPASLVGRYLYLDYGYHAWSMGYSDSQKRRNLEV
                     LWAQSPNHESWCTLLHQEKIDAILVGPGENNTEDGRINVAESYVVSKLQPTFISEDNW
                     KIWMVNQICPKK"
BASE COUNT         4232 a         2591 c         2333 g         3936 t
ORIGIN      
        1 gcagtcttgc gtttagaatg ctaatcgtga ctgaagaaac aaaaaaacca accaagcttc
       61 ctcaagtaga ggcgattttc tctggggaaa ataattcaaa tcaagccgag gttatcaaaa
      121 cgacaaaatc tcccgaaatt cgcccaccag agagatttat tttacctgct gttcccatta
      181 gagaaggcgt tctgtttccg agcaccgaat cagttttaac tttcggtaga caaatttctc
      241 tatcaagcat caatcaagtt acccccaagc agaactctgt tgttctgatt acccaaaaac
      301 gaccagacgt ggaggaacca aaagcggacg acatgtatac cataggaaca ttagccttgg
      361 tagataaaaa aataaaaacc gatgaaagta taaatgcttt agtgagagga attggtcgag
      421 taaaaatcat cagatttata caacttagtc caaaaatgct tgttgaggtt gaaaaacttc
      481 aagacataga gcaaaaggat gaagaatcag cagcgctcac taaccatctt caaaaagaat
      541 ttcgcagagc tgtccatatg ggcaaaccgg ttgaattcct aaattttatg aagctgatgg
      601 gcggtgtcac tgatggagaa ctaacagatc aaatagccag caccttaaac gttgaaacaa
      661 gtgaaaaaca atcaatcctg gaaaccttag ttgtaaaaga gcgaatgaga atggtgatag
      721 ataagctagc tcatgaaatg aaagtgcttg aaattgaaaa agacgttgta aacaaaactc
      781 aaaagaaatt tgataaaagc atgcgcgaga atgtattgag agaaagactg agaacaatcc
      841 aaaaagaact tggagaaatt gatgatgaag aagagattgt agatgaatat gaggaaaagt
      901 taaaaaaaat tactgtagac aaagatatca aaacaaaaat ttcaaaagaa attaaacgac
      961 tcaaacagat gtcacccaac aatcctgagg ctggctatgt tcgaacttgg ctagatacaa
     1021 tttttgaact accctttggt aaatattcca aaaccaaggt taaaatccaa caagctgaag
     1081 aatcactcga gaaaagtcat tatggattgg aaaaagtcaa agatcgcatt ttggaatata
     1141 ttgcagtact tgaactaaaa cagcgaactg caaaaaaaga gggagaccga cgattaccta
     1201 ccatactatg ttttgttgga ccacctggag ttggaaaaac tagcattggc aaatcgattg
     1261 ctgaaagttt aaaacggaag ttcgttaaaa tttctttagg cggaattaga gatgaagcag
     1321 aaattcgcgg tcatcgacgt acatacgttg gcgcaatgcc tggcagaatc atcactggaa
     1381 tgaagcaagc cggttctatg aatccagttt tcatgcttga tgaaatcgat aaaattggta
     1441 atgacttccg gggtgatcca tcagccgcac tacttgaagc actggatcct gaacaaaata
     1501 gtagttttga ggatcactat ctggatatgc attttgattt atcagaagta atgtttatta
     1561 ctaccgccaa taccctagat acaatcccac ccgcacttcg tgatcgtttg gaaataattc
     1621 gttactctgg atacactgaa gaagaaaaat ttaatatagc gaaaaaacat ttatttcata
     1681 aagtgagatc ggcaaacgga ttaactggta atcatatgca gattaccgat gaatcaatac
     1741 gctttatcat tcaacgctac accagtgaag ctggtgtccg agacttggaa agaaatttgc
     1801 ataaaatcat gagaaaactt gctcgtaaat tgacagaatc tactaaaacg acaaaaacca
     1861 ttgttacacc caaaattgta agagagtttt taggtccaga aaagtttgac gttgaaatga
     1921 gcagtaaaac tgatgtcatc ggccaagcaa ctggtttggc ttggacatct gttggtggag
     1981 acactttatt cattgaagtt tcgcttaccc caggaaaagg cgccgttcag ctaaccggaa
     2041 aactcggtga tgtcatgaaa gaatcagccc aaactgcata cacttttgtc aaagccaatg
     2101 cagaaaaatt cggcattgct cctagccgga tcgcaaaaac tgatattcat gttcacgtac
     2161 cagagggagc tgtaccaaaa gatggcccgt cagctggtgt aacaattaca acagcaatag
     2221 tttctgcctt tacgaatatc gcagtaaaaa aagatattgc aatgacagga gaagtcacgc
     2281 ttcgaggacg ggtgctacga attggtggtc tgaaggaaaa aagtcttgct gcagatcgtg
     2341 caggcattag cactattatc attcccaaat caaacgagcg cgatcttgaa gaaattccaa
     2401 aaacagtatt gagtaaaatc aaatttatcc ctgttgagca agttgaagaa gtaattgctc
     2461 tcgcattggt taaaaaacca aaagcgagaa acatacaata atctttacat atggtacact
     2521 gtcggtgtat gcgctctttc ttcagagtaa tttttaccgt tgtatttatc ctttttaccg
     2581 gactaagttt ttccgcatgc actcagattt ttgagaaaga agccttggct ggaattcaaa
     2641 ttaccactgg tcaaatagaa agcgttgtat ttattaacgg tcagtatcta gacaaatccc
     2701 ccttagtgcg aaaaaatctt aaacctggtg aatatataat cgaaattaaa cccgcgaatg
     2761 atgcattagc ttcatatcaa acatcagtta cattgcggca agaacttctg acggtgatta
     2821 catggaatcc agctgcaacg cctgagcaaa gtgggggagt agtctacgaa atggaacccc
     2881 ttccaatgaa ggatgctacc gaagtatcat tttcgacagt tccagacggc gcaattatac
     2941 aattgatgga taaaccaaaa gaaattgcgc cctatacctt taaaaacgtt tcccctgggg
     3001 aacaggagta ctcaattagc ttgccctctt atgaacaaca aaaacataca ataaatattg
     3061 tacctgggta ccgcatgctg atttcactca agctcgctaa acaatcgtca atattgctca
     3121 tgcccactcc aacgattact ccagtcagca gcgccagttc aagcgctact gcctcttcat
     3181 cgggaacagt tacaattcta aaaaccaact tatttgtgga tggagtagag gtactgagag
     3241 tccgagatct gcccaatgta tctggtactg aggtaggatt agctaaagtt ggtgtcactt
     3301 atccgtattt aaacgagaaa aaagatggct ggtttaaaat caaactcgca tccgctagtg
     3361 gctggataag cgggacatac gctaatttga aagagtagta gtctgcacat ctcgccattc
     3421 ataaattgat aaggtcctgc ctgaattttt aagttgagca aatgtatcgt ttagccatcg
     3481 gtcgtactca tgtgaataaa ctgcaggctc tctcacaacc agagtctttg gatctgttat
     3541 ccagttccag taatgcagta gctgaattga cattagtcca agtacacccg ttaccacaga
     3601 acccaaaaat atcatcctga taactggttt aatgcagaat ttagaattat aaaaacgttt
     3661 taaaagaaga tacaaactgt gggcacccag cgtgcccatg aaatagatat acggcaaagc
     3721 gtgtatcgtt ctggctgcat caggagtatt gttactaaat atattaactg gaactattaa
     3781 tagtaatccc acccccacca accaccagcg cttccacaca gtataaatga taccaaccat
     3841 gaatagtaac acgactacgg ggtggataat ccaactatca agaggaacat aacgactatt
     3901 aatcccaatt gaccactgtt ctggttgaaa agttaaaaaa cccagccagt tattttttag
     3961 attgaatagt aatgcctctt taacatcgat ctcgctaaaa atagaaaccg tagttattct
     4021 gccgaactga ttaaccgaat ctataaaaaa cattggcatt attgagagac tgagtaaaat
     4081 caagattatt agtaactgca tgattttttt tgataatcca attttttctt ttaagctgac
     4141 tacaatgaga gcaattaaag cagtaaaagc aataattttt cccggatgat atagataggg
     4201 agatcccatc gctacaagca ccatgagtat tttttgaaac ccatgttttt ttgagtgtgg
     4261 ggtaaaagaa tacagtaaaa gtattacacc aagacaatta gcaatatttt cccagccgct
     4321 tcgagaaaag ttaagaaacc aaggatctac cattaaaagt aatgttgaga gtaaagcaat
     4381 taccgcatta ttcagccaac ttttgacaag cagataaaaa gctaataaac agataatgct
     4441 aataagtgct ggacccattt ggactgccca atatggcatt cccattacct cccaagggat
     4501 ggcaatcatt agttggttaa taatatgaga tccattccaa ttggttgaca gaagcgaaaa
     4561 atttccagca aaacgatgtt ctaaatatat tcgaattgtg tcgacggagt cagggtgtaa
     4621 atttccaggc accgaatcta aaaaagcaaa acgagttatc actcctagta acaagataat
     4681 aactagatgt gcgatcgtaa ctttattcat tcgtttgatt ttttgtaagc aacagcggat
     4741 caattacctc atatatcgtt gatcctggtt tcaaccattc tattcggaca tttctagctc
     4801 ctcccaaatt ttgatagcta actaccaact cataaatacc cgattcaaga atgacggttt
     4861 ggacccaatt cttctcaggc tgttgactga atatgatttc cccagatgaa cttgctaact
     4921 gcatatccac gctatcatct gcgctacctt ttaaactata ttctccagat tgtgtaattg
     4981 caatttttcc cttccaatta cagttcacta tattttctgg aaatgtttgt agactatgat
     5041 aaattcccaa cgactgcttc tgcaataatt gcatttgtcc attgtcacag gtaactgata
     5101 atccaacaag ttctgatgga gggcgctttg gtaatagttc atagacgatt ggtcttgttt
     5161 cttgacctgt ctctagttca aacatattca caattgtaaa atattcttcc aagtatgatt
     5221 gtaatccgac cgcggaactt tccaaaataa gagttgctgg ctcgtctatt tctaataacg
     5281 catctatatc aaacattcga aattctaagt ttgcctgctt tggtagggta agaaatattg
     5341 ttgatgaata aagatagtca gttgataaaa aaacttgatt gcctaactct ctgtacttat
     5401 ttgcagtttg ggcaattatc tgttcgggat aactaaatgc ttggagtagc gaaaagtttg
     5461 gctgcaacat gaataaataa tatcgagcaa gcaacagagc cactgaacta ctgaacaata
     5521 acaataagcc gggttttaaa tagcttaggt taatttgcga ttttttgatt aagcatgaag
     5581 aaaatggaat cttaaagata attactgcta tgcagattgc cataattggt attgatccaa
     5641 atatccgaaa gtaggaaggg gcttcttcac tcaggactcc gccagtcata gttgcaatta
     5701 accccagcaa taaaaatatt ccctgtttcc tagtcgactt ttttatacat aaaattacaa
     5761 tcgacgctgg aattaataga cccaaatagt atggaaacaa cgtggattgt agggggttat
     5821 gacggagatt ttcatcaatt gcatcataaa agattctcaa ataaataatg ctatttttgc
     5881 tcagcgcgtc catattagat ctcaatgatt gatccgactg aataatactc acttccttga
     5941 ttctccaaat gaaattgaag ggatcttttt gcatttctag tactattgga aaagccacta
     6001 aaaatatgac tgcaataaaa gataagctac caataatttt tttttgaagt tttcgtttgc
     6061 tatataccca gtaccaaaat atatacagtg gaaaaagcaa aacatacgat cttgctccaa
     6121 catagaaata taaactcaaa cccatcacgc aagcagcgcc cattatgtaa agattgcttt
     6181 tccaattctt cgactccaca ctcatccaaa ggaaaagtag tgaaagtaat tgaaacatag
     6241 ctgttatcac cgcaccgtgt ccccaacgtg caatatgaac cgaggtcaca tttaaaccat
     6301 acagtatcaa acttgctaat ccaacaattt ttgaggaaaa caacttttta cccagttgat
     6361 atacaagcgg caatgaaaca attgctggaa taatccatgc aagtttaatt atacttattg
     6421 aaatcccaaa caggcgaaac aaaaacgcca cataataaaa aaatagtcca ggtgtggcat
     6481 accaaccaaa accgttccat ggaattgaga tgttccctag tgcaatctga gctgctggta
     6541 cagagagatt aaattcatcg ataaaagttg ctggtggaat cttatcaata gcaatcaccc
     6601 tagccaaagc cagaatgatt ataattacta ccacaaacaa ttggtaaagc tttgtcatct
     6661 tctttatgat aatttaatta tggtattttc tcatagtatg aaacaaataa tatatagact
     6721 cttcttcata ggcatcatac tagtgcttgt tggatcaaga ttttttggca acaattacga
     6781 taacctacga catctacatc cagatgaacg gtggttggta atggtggttg gaagattgca
     6841 tttttttgac aatttgaatc ccgatttttt tgcgtatggt agtctcccac tgtatgtgtt
     6901 gaaagccttt gcgcaactga gtgatgcaac tttccagacg cagtacgaca attatgacgg
     6961 cctgctcata ttgggtagga gtcttgtcag tataattgac attgctaccg cgatagttgt
     7021 gtttctaata acaaggaaaa taacaaaaaa tactttaagc gcgcttttct cgatgcttgg
     7081 gtatgcacta ttattttttc caattcaaaa cagcaatttt tttatagttg ataattttgt
     7141 caacctattt ttcagtgcaa cacttttatt tcttatccgc tatgttgaaa agccgaattg
     7201 gcagaggctt actattttat cggtgatcta tggtttgcta ttagcgagta aaatcacccc
     7261 gataatttta ctcgctccaa ttgtttttgt cttattttca atgcctttcg ctaattcaat
     7321 tctccataat cggctacttt tatcaatcaa atcaaaatta atacaacttt tcagttctct
     7381 aagctcaata gttaatcata aaacacatca tcctcggttg ctgttttcga gtttggtcaa
     7441 atcccttgcc aatgtgctac tttttttctc gattaccata cttacagttt ttataggcat
     7501 gcccttcact gttattaatt acgaacaatt tatcagagaa atcagcgccc aaatgacgat
     7561 gaacagcaat gcatatattt tcccctatac actccaatac gttgatacca ctccctacct
     7621 gtattatttg aaacagatag cgatttgggg cgcgggacca attttatcga tgattgcact
     7681 agtcggtctt ggcttatcaa tcacccaact tgtgaaatat tggaaaaata aaggattgct
     7741 tcaagctatc tttcagccac ttacaatcta tttatttgcc aacctcatct attttattgt
     7801 gatcggacgt tctgccgtga agtttatgcg ctacgtgttg cctttatatc cctcaatagc
     7861 tgtgtttatt ggtatcgcgc tcggtttcat gtttagttct aaaaaatttc ccaagctatt
     7921 tcggtggacg ctcataagta ctatcttagt tcttgcaagc ctatggacat ctgcatttgt
     7981 atcaatctat tcccaaccac acacccgtga aactgcttcg ctttggatgt tgcaaaatat
     8041 tcctccagga tctcgattgg cagaagagca ctgggacgat cggttaccgt tgtattctgg
     8101 agaattattt gagtacatcg atctacctct ctacgaatta cctgatgatg caaataaatg
     8161 gaagttaatt gcaaatcgat tggagcaggc cgattacatt gtaatgacct ccaatcgact
     8221 ctatggctca cttcccaagt tagccgattg ttcaaatcat gagaaatgct atccattaac
     8281 agcacaatac tataatgatt tgtttgcaga aaatctgaac tttgttaagg ttgctgagtt
     8341 tactagtaga cccacaatct ttggtatcga aatatttgat gataatgccg acgaatcatt
     8401 tactgtctac gaccatccaa aagttatcat atttcaaaaa ggtagccggc gatgaaaagt
     8461 cgctattgtt ctttactaat ttcaataatc atatgcatct ttactgggtg tttgttattt
     8521 tataatctta ataattaccc accaacactc tacaactggg aaaattattc tgtgtggaat
     8581 atttttcaac aaagattcaa cgagccaaca atttcaatgg catcctattt tcaaagagcc
     8641 gacggactta tgacagaaag tggtttttca cccatagttg gattgccaat gtacctaatt
     8701 acagagattt ttggctattc tctagaaacg cttagaattt ggccagctac cctgacaata
     8761 attggagttc ttgttttttt tctatttatt ttaaaagtgt ttggtaaaaa aataagtgca
     8821 atcgccacta ttttactgat tacttcacag ggttttctgc tttacggaag aactgccact
     8881 aacgtagctc ccactctttt ggctgaatca gttacagctg cgcttatcta ttatttttta
     8941 ctcaaaccaa aatcatttgc tttatttctg tctgcgttag tcatggtcgt tattaattat
     9001 tttttttatg cacccattag gtttttcacg ccgattttat tattctgtat tgctcggtac
     9061 ttactattgc atgtgacaca cctcttatct aataatgatg cggtaattaa atctggagaa
     9121 atacttaagt tgctagtcat cggtgtattt gtaagctttg caattgtgat tatttacttc
     9181 tcaaaatcac tctttctaaa ttactttcat gcaagaggag aacaaatatt tgcaagtatt
     9241 actcaaataa atccatccgt atatcacatt aagaatccaa caatgcagat ttttgtaaat
     9301 actaaacaat atttgagctt gctattctca attgactcta aaccagtaat tattgactac
     9361 ggaaatcact atggacaaat gatgcacaga gctcttgttc cattttttat cttaggcttt
     9421 tttgtaatga taaatggatt aaaaaagaaa aatcgaaaat accgattact agctttctgg
     9481 tttttactta cttgtgtacc catcctcctc actaacaacg tacatattgg tagactcttt
     9541 ctgcatctgt caccattatt tgtggtggta gccatcggtt tatccaaact aaccgaagtg
     9601 attattagta ggttcaattc agtatttcct aacagacttg cagtgagtaa aagagcgagc
     9661 tatacatctc tttcactctt tttactgatt atttcagtga ttgaaattcg cgcatatttt
     9721 ctaactccac cgactattga tcacaacata aaagtattaa aagaaaatga atctgagtat
     9781 atttccaaaa ctatttacgt cataaatact gaggcttcaa cgttacattt ctgggaagtg
     9841 gctttttact tacgtgaaaa attctatttc aaagacggca tgaatcttaa gcctttggat
     9901 atggcaagaa atcaagaaca agttggtgag ggttatttat tccgcacaca aggtagcctg
     9961 gacacattag cttcggcttg tgacaattct gattcggctt tgatagtagt tggaagaaat
    10021 agtaaattca agtcgccatt agaattaacg aactgcagag catcaactct acttttgtat
    10081 caatagatta tttaaaacag cttttttaca attcccaaag cgccttgctt ccatccaaca
    10141 gtgtgtgtca cgccgctttt tctacttttc acttcatcat aatgtttttg ataccactgg
    10201 aatgatttga taagcgcctc tgcatttgag taccttggag accattgcaa tactttttcc
    10261 agtttttcaa tcgacacaaa agaatccttg tccgcagtgt cataaaccca ctgatagagc
    10321 ggagagagac gtaataattc taaaagtcgg agaatcattt taattggagt agctggagtg
    10381 ggaaagagcg aagatccaga acccgccttc ttaaataatt gatccaaatc cttacctacc
    10441 gtctgaaact tcttcgctcc aatattgaat gcatcatcaa atctactagt attcttcgtt
    10501 acattagaga aacgatatat cgcttcaact aaatcatcaa cgtcgagtaa ttgatagcgg
    10561 ttttcaccat ttccaataac cggtatgcgt ttgccatcat gtatccaatc aaaaagaatt
    10621 tcaaagactc ccaatcgttc tgtgcccaca aaagtttttg gacgaataat tgttactggt
    10681 tggccagttt tgatcgctgc aaaacaatga tcttctgctt gaatcttact gatgccatag
    10741 ggaccaactc caattctggg atctgtctct acgattgggt gctttttagg cacaccgtat
    10801 accgcagtgg atgagatata gataaagcgt tttatcttag ctctacctgc ggcggcgagc
    10861 atttgacgag taccttcgac gttggtactc actatttctt cagcggtcca tagcggaagc
    10921 gccgccgccg catgaataat cacatccata ccttttgtcg ctgctgttgc aatctttgca
    10981 tccctcacat caccagaaat taattccatt tttgagtcat actcttcttt gggaaatgga
    11041 gcaatatcaa gcaatctaac atgatggcct tttacgtgat agtaacgcgc taaatgtaat
    11101 ccaagaaaac ctgctccgcc ggtgattaat actttcatag catctttctt atctcttcta
    11161 ctacagcttc aaagctcaaa tatttatcgg gataggcaaa atgaaaatca gccacattac
    11221 ttatccaaaa tattcgcgcc ttagtgtttg ttgctaaata ttgagcaata ttccaattgt
    11281 cttccacgta acaatctaac tttagtgaat taatcatttt tgctttaaat tcatgcggtt
    11341 gttcatcaga ttcagaaata aaactttgtt taataaattg ccgcgcgtgg atagatttca
    11401 aacgcctttc aaaatcagat ttcagaaatg tacttctgcc cgtaatcacg tatgcttcaa
    11461 ttttattttc tgacaccatc tgacgaactt gttgaagtcc gcgcgcagga aacaaactag
    11521 attgatgagc aagccaccaa aaccatctct ccaactttct ttttggatga taaaaaacta
    11581 attgagttga accattttgt ttttttttgc ccctcaaata acctaaaaag ggccgaagga
    11641 cgcgagcggg gttatacaga actacgccat caaaatcaaa acctaccttt aatgtttttt
    11701 ttgttatttt ttggggcata tttgatttac catccatatt ttccaattat cctcacttat
    11761 aaatgttggt tgtaatttgc taactacata actctcagca acattaattc tgccatcttc
    11821 agtgttgttc tcgccaggac caactaggat tgcgtcaatt ttttcttggt gtagtagtgt
    11881 acaccatgat tcatgatttg gagattgagc ccaaagaact tctaaatttc tacgtttttg
    11941 cgaatctgag tagcccatac tccaggcgtg gtatccataa tctaaatata aatatcgtcc
    12001 tactagtgaa gccggactat acatatacga tgcggttaaa aactgagctt gaggctgcgt
    12061 gttattttta atccattgct gcattggaga ttttttccag tctgaaattt ctccatcata
    12121 atcattggta atagggaaca catcaataat gcccgaaaaa gttaagccaa cgagcagcgg
    12181 tacgattatg attaacaaca ccttatgttg tttgactaac gacactagaa acccagctgt
    12241 ggtaatttgc aaagcgataa taaaaaaagt gatcaactta tgattgttaa tcatatctgc
    12301 gctgaatcgg aaaagattgg caattacaaa aaaggggagt gcagtccaaa tccaccattg
    12361 tttctttatc actgcccaaa tgagtaacac gggtaataat agccaataca acccaaggtt
    12421 attaaaccag taccaattta ttgccatgag tgttttttct ggacttaaat aacctatttg
    12481 ccatatgggt agtgaaccag attgcggcgt aaatacttta aacacgataa tagaaaccag
    12541 aacagctaaa ccataaacca ataaaacaga ctttgatttt ggccaggtaa taccaatcca
    12601 aataagcgta agcggtacca aaataatgta agctgcttga tgtaataaag gaaatatcac
    12661 aaaacttatt acaagcaagg gttgccacca ataccgatta aatagttggt tactattttt
    12721 cttgctggaa atttttttca ccgctcccaa aacacctcca aataaactag tgaggctgcg
    12781 actccaacgc tcttttaagg attcctttgg tatcacgctt aatctgacaa gtggatacag
    12841 aagtagtaaa aacattccat aactcaagcc caaatgacgt tggttagtaa aaatattcca
    12901 attccaaaat gcactaacca gcctaccact ccacggacca aaactggcaa actgactttg
    12961 ctgtggaatt gacgtaagtg cttcccaggt ccatccgtgt tgattaaaaa attctaccca
    13021 tgctagcgat ccattgaaga gaaacagtat cacagccaag atccccgcca ctgcagaacg
    13081 aaaaagcgtt tt
//
LOCUS       DAOQZP010000029        11944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_5840_length_11944_cov_5.398856, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000029 DAOQZP010000000
VERSION     DAOQZP010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 11944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11944
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5840_length_11944_cov_5.398856"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..880)
                     /gene="rplC"
                     /locus_tag="PKH60_02960"
     CDS             complement(50..880)
                     /gene="rplC"
                     /locus_tag="PKH60_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNQ17039.1"
                     /translation="MLTDFYATKTGMTQAWTADGKRLAVTRLKTPDNVVSAVLSPNIL
                     EVGYGQKKEKNVTKPLSTRFKKSGFSFMPAKLGGARWTTQEGEEQPKAGTTITAASVL
                     SVGDVVEIRGITKGRGFAGAVKRYGFHGGPKTHGQSDRTRAVGSIGAGTSPGRVWKGK
                     RMPGHYGVETKTIGGLVILHIDEVAKEIWVSGPVPGHLNSFVLVRKTGREQEIKLNNK
                     ASGITVPKVEETKTEVITEVEETTAEVASNEVVETSATESVATETAGADSEVTEKPTT
                     "
     gene            complement(979..1320)
                     /gene="rpsJ"
                     /locus_tag="PKH60_02965"
     CDS             complement(979..1320)
                     /gene="rpsJ"
                     /locus_tag="PKH60_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNQ17040.1"
                     /translation="MTAATKNNSSSSATYIIRVKLKSYDHRVIDEASKKIVETVLATG
                     AQVNGPVPIPTHIKSFTILSSPHVDKNAQEHYEIRTHKRVIDIVNPTSKTIDSLMHLE
                     LPAGVSIQIKS"
     gene            complement(1436..2632)
                     /gene="tuf"
                     /locus_tag="PKH60_02970"
     CDS             complement(1436..2632)
                     /gene="tuf"
                     /locus_tag="PKH60_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNQ17041.1"
                     /translation="MADASKFVRNKPHVNIGTIGHVDHGKTTLTAAITTTLSAKMPSA
                     INKAMKYADIDNAPEEAARGVTINISHIEYETEKRHYAHIDAPGHADYIKNMITGAAQ
                     MDGAILVVAATDGPMPQTREHILLAKQVNVPQIVVALNKVDMVDDTELLDLVEMEVRE
                     LLTKYGFDGDKTPIVRVSALKALEGDATYQEKIMELMKTVDDYIPEPVRDLDKPFLMP
                     IEDVFSIKGRGTVVTGRITTGIVKVGDEIEIVGLRPAQKTTVTGVEMFRKMLDQGQAG
                     DNVGVLLRGTGKDDVERGQVLAKTGTATPHTEFEAEVYILTKEEGGRHKPFFTGYRPQ
                     FYIRTTDVTGEITLPEGVEMVMPGDNAKMNAKLIVPVALNEGLRFAIREGGHTIGAGA
                     ITKVIK"
     gene            complement(2686..4815)
                     /gene="fusA"
                     /locus_tag="PKH60_02975"
     CDS             complement(2686..4815)
                     /gene="fusA"
                     /locus_tag="PKH60_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNQ17042.1"
                     /translation="MSQFASSDRNKIPLERIRNIGIIAHIDAGKTTTTERILFYTGRT
                     YKIGEVHEGAATMDWMEQEQERGITIVSAATTTFWTPYFDSHLPKVQYRFNIIDTPGH
                     VDFTAEVERSLRVLDGGITVLDASEGVQSQSETVWRQADKYKVPRICFVNKMDKLGAD
                     FDATVEDIRIKFGVKPAVMVLPMGAENDFRGVIELIDRKALFWKDSATDTEPEVMEVP
                     EQYKEKMETARAALVEAIAETDDALLEQFFAEKEIPINDLKAALRKAVISYQLVPIFA
                     GSSLKNTGIQPLLDAVVEYLPSPVDIDHIEGTNPKTQETEVRKLLPSDPFCALAFKIQ
                     TDPHVGKLTYCRVYSGTLKAGSYTYNTTKREKERVGRLLLMHANQREEIDEARAGEIV
                     AIVGLKLTKTGDTVCDEGKPIILEGITFAEPVIYLAIEPKTKSDQEKMGMALAKLTEE
                     DPTFEIKSNPETGQTVIGGMGELHLEIIVDRMKREFKVDAIVGKPQVAYRETITQAAE
                     GEGKYIKQSGGRGQYGHVMIKIEPKERGTGYEFVNGITGGVVPREYIEPTNKGIQESL
                     SRGFLAHFPLSDLKVTLYDGSYHEVDSSELSFKMAGSLAIREATKQAQMVLLEPVMKL
                     EVSTPDEFMGDIIGDLGSRRAQIQGTSTRGNTVIITALAPLAEMQGYVTILRSMSQGR
                     ASSVMLPSHYDIVPKNITEKIIEERSR"
     gene            complement(4910..5386)
                     /gene="rpsG"
                     /locus_tag="PKH60_02980"
     CDS             complement(4910..5386)
                     /gene="rpsG"
                     /locus_tag="PKH60_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNQ17043.1"
                     /translation="MRTKRAKILPVVPDAKYGSTRVTKLINYVMREGKKTVAQKQVYK
                     ALELLETKTKKDALTAFDEVLQIITPPMEVRSRRIGGAAYQIPVPVKPRRGMSLALRW
                     LVQEANKRPNKQFHTFAEKLEAEMFDALNNQGGSIDRKNTSLRMAEANKAFAHFRW"
     gene            complement(5400..5810)
                     /gene="rpsL"
                     /locus_tag="PKH60_02985"
     CDS             complement(5400..5810)
                     /gene="rpsL"
                     /locus_tag="PKH60_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNQ17044.1"
                     /translation="MPTFNQLIRKGGRKRKPSRVKASALRRSYNSKSGQFVSTTNPQK
                     RGVCTVVKTMTPKKPNSALRKVARVRLSNKTEITAYIMGEGHSLAEHSIVLVRGGRVK
                     DLPGVKYHIIRGKHDCIGVQNRKTSRSKYGVKKS"
     gene            complement(5841..9560)
                     /gene="rpoC"
                     /locus_tag="PKH60_02990"
     CDS             complement(5841..9560)
                     /gene="rpoC"
                     /locus_tag="PKH60_02990"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNQ17045.1"
                     /translation="MNNPNTQNQKTRYIIDFNALEIRIASPEVMRSWSHGEIRKPETI
                     NYRSLKAESDGLFDEKIFGPIKDFECYCGKYKGARYRGITCDKCGVEVTQSKVRRERM
                     GHISLAAPVAHIWFFKGAPSKLSLLLDISPKNLNSIIYFSKYVVLEVDGEEKAMVMQS
                     LDENLQVAIDSLKTSIKEEVEKVKEQLTDDKKQLKEKVKSKETLQLKTAEIEVKAKNK
                     LIALKQQLDTHLEQTEEIYQTVKSMVKRITRGTILSEDEYLKLDEYDASSHLRVGMGA
                     ESILELVEALNLDELVEKLRKEMIEAKGARRIKATKRQRVVEGLRKAQIKPAWMFLNV
                     LPVIPPDLRPMVQLSGGRFATSDLNDLYRRVINRNNRLKHLIDLGAPQIILRNEKRML
                     QEAVDSLIDGSQARQSRRATRRPLKSLSEMLKGKQGRFRQNLLGKRVDYSGRSVIVVG
                     PELKLNECGVPKDMALEIFKPYVLRELIVKGIAPNVKSARHLIDRREPEVYDVLEQVT
                     INHPVVLNRAPTLHKLGMQAFYPILVEGNAIRLHPCVCAGYNADFDGDQMAVHVPLTQ
                     QAQEECRDLIMSTRNLLKPANGEPITIPNKEMAMGVYYNTVMDEGDAYGVVFSSPDEA
                     YHAYEVEMIGLRQPIRVRRQTDGESEIITTTIGRLQFNDLLPASFPFLNQTVTAGMIK
                     SIVTQAIEKEDWQVVTLLIDSIKNLGFTSATLSGLSVSVSDCEMIPEKNEMIEVANKK
                     AEVIQSQYQEGLISDEERKRLSFDLWIKTTDEVADKTWQSYSEDNAIKVMINSGGTRA
                     SKDQVKQLAAMRGLVVDPLGNIVEMPTKSNFREGLSIFEYVTSARGSRKGLTDSAIKT
                     ADAGYLTRRLVDVAHDMIIRAEDCGTDKFVVIEKEPRHDIFASRIAGRYAAQDVIAGK
                     TTIVSAGDLITPELAAVIDSKNITSVAVFSPLSCDLPKGMCAKCYGWDFSTRQRVEIG
                     VPVGVIAAQSIGEPGTQLTMRIKHSGGIVGLDVTQGLPRVEELFEARVPKNAAVMADI
                     SGQVSIEEESGKLMLTITPGDKSIEPSVYEFPLQTPIIVKTGDLVHLGQSLTDGPMNV
                     KELLALRGLLAAQQYAINAVQGVYESQGIPINDKHFEVIVRKMSDKLQIESIGDTDFL
                     TGEVIERGRFIEDNEETIAAGGEPATAKVMFLGITRSSLFTNSWLSAASFQHTKNVLT
                     DAAASGSVDKLEGLKENVIIGRLIPTSKERAQLEM"
     gene            complement(9557..>11944)
                     /locus_tag="PKH60_02995"
     CDS             complement(9557..>11944)
                     /locus_tag="PKH60_02995"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNQ17046.1"
                     /translation="IREKMSLTKTEDVLSPAALVNARPLIATISEFFRRNRLSTILDQ
                     TNPLSEIDNLRRLSVMGTGGVTRERASFSMRDINASQYSRIDPVRSPEGPNIGLVTYL
                     ALYAKVNKHGFLEAPYLKVVADKGKMKVSDEIVYLAADEDEDYKITHAGIDIDEKGYI
                     TDEWVAMRTMNKFIEGPVEEVQFIDVLPQQVVGTSASLIPFIAHDEANRALMGTHMQC
                     QAVPLIKPQAPIVGTGMEMAVATAMGRTVLARHPGEVVYVDGDRVEVKLNKSVLAKIN
                     PAITATETIEFQDDREIYHISKFERTSQSTSYTQKAVVSLGDKITVGDVVIDGPCADQ
                     GELALGANVLISYASFDGLGYEDAIVISERLVREDVLTSVQINEYRAKVMDTKLGPEE
                     LTSDIPNVSDVYLSNLTTEGIIRVGSVVDSGDILVGKIAPKGETELSPEERLLRAIFG
                     EKSREVRDTSLRMPHGEQGTVIDVQILDSDMGDELDPGTIKEVTVKVAQIRKITVGDK
                     LAGRHGNKGVISKIVPVADMPHLEDGTPVDIIISPLSVLARMNMGQLLEAHAAWPAQK
                     QGYSIAVPAFEQFDESKIWQELERSGLPVTGKAQLFDGRTGEPFSETTTVGIGYILKL
                     SHMVEDKMHARSVGPYSLVTQQPLGGKAQMGGQRLGEMEVWALEAHRAAHTLQEMLTI
                     KSDDVIGRAKAFEAIVKGEPIPSPTVPESFRVLMRELNSLGMDVVPHETKEEIVEVEI
                     PNPEALLVEDILPDAELLADGEDESEKELEISEEPTDDLGDLEPVELEIDEEKAL"
BASE COUNT         3127 a         2767 c         2251 g         3799 t
ORIGIN      
        1 ctgacaacgc aggttgcttc tttgccagtg ggagtgattt ttgttatgtt catgttgttg
       61 gtttttcagt tacttctgaa tcagctcctg cagtctcagt tgccactgat tctgtagcgc
      121 tagtttcaac tacttcgtta gacgctactt cagctgttgt ctcttcaact tctgtgatta
      181 cttcagtttt tgtttcttca acttttggaa cagtaattcc actagcctta ttgtttaact
      241 taatttcttg ttctctgccg gttttacgaa ctaagacaaa gctgttgaga tgaccaggca
      301 ctggtccact cacccaaatt tccttggcta cttcatcaat gtgcaaaatt accaaaccac
      361 caatggtttt cgtttcaaca ccatagtgac caggcatgcg cttgcccttc caaactctac
      421 caggacttgt acccgctccg attgatccaa ctgcgcgagt tctatctgat tgaccatgag
      481 tttttggccc accgtgaaaa ccatagcgtt taaccgcacc tgcaaaacct ctaccttttg
      541 taatacctct aatctccaca acatcaccta ccgataaaac tgaggcagca gttatagtag
      601 ttccggcttt gggttgttct tcgccttctt gggttgtcca acgagctcca cccaattttg
      661 cgggcataaa tgaaaaaccg ctcttcttaa atctagtaga gagcggctta gttacgttct
      721 tttctttttt ttggccgtaa ccaacttcga gaatatttgg tgaaagtacc gcagaaacta
      781 cattatctgg agtttttaag cgtgtcacag ccaaccgctt gccatctgca gtccaggctt
      841 gtgtcatgcc agttttggtt gcgtaaaagt cggttaacat agactcgatg acaacccata
      901 agtgagttga acatcctatt atttttgata ctaatctttt tcttcaccat agagatggtg
      961 ttcattccca agtacgcctt aggatttaat ttgaattgat actcctgcag gtagttctaa
     1021 gtgcataagt gaatcaattg ttttacttgt tgggttaaca atatcaatca cgcgcttatg
     1081 cgtgcgaatt tcataatgct cctgagcatt cttatccaca tgaggcgagg ataaaatagt
     1141 gaaactttta atatgggttg gaattggcac gggtccattg acctgagctc ctgtcgccaa
     1201 cacagtctcg acaatttttt tacttgcctc atcaatcaca cgatgatcgt atgattttaa
     1261 cttcacccga atgatatagg ttgcggacga acttgagtta ttttttgttg ctgcagtcat
     1321 atttctttgt tactgtgcgt aatcctttac aagacaagtg gcgattatac ctagtatctc
     1381 gtaaaaagta aatcccccct gtatcttttg gatacagggg ggatctttgt catacttatt
     1441 taattacctt tgtaattgca cctgctccaa tggtgtgacc accttcacga attgcaaaac
     1501 gcagtccttc gttaagtgcg actggcacaa ttaacttagc gttcattttt gcattatcac
     1561 caggcataac catctcaact ccctctggaa gtgtgatttc gccagttaca tcagtagttc
     1621 tgatataaaa ctgtggtcgg tagccagtga aaaatggttt gtgtcgtcca ccttcttctt
     1681 tggtgaggat atagacctca gcttcaaatt ctgtgtgagg agttgcagta ccggttttag
     1741 ctaaaacctg tccacgttct acatcatcct tgccggttcc tctaagcaag acaccaacgt
     1801 tgtcacctgc ttgaccttga tctagcattt ttcggaacat ttctactcca gtaacggtgg
     1861 ttttctgagc tggacgaagt ccaacaattt caatttcatc accaactttg acaataccag
     1921 ttgtaatacg accagtaacc actgtgcctc gacccttgat tgagaagacg tcctcaattg
     1981 gcattaagaa tggtttgtcc aaatcacgaa ctggttctgg aatgtagtca tccacagttt
     2041 tcatcaattc catgattttt tcttgatagg tagcatcgcc ttcgagagcc ttgagtgcac
     2101 tgacacgaac aatgggagtc ttgtcaccat caaaaccata tttggttaat agttctctga
     2161 cttccatctc gactaaatcg agaagctcag tgtcatccac catatcgact ttattcaatg
     2221 ccacaacaat ttgaggcaca ttgacttgct tggccaagag aatatgttct ctggtttgag
     2281 gcattggccc atcagtggcg gcaacgacta aaatcgctcc atccatttga gctgcaccag
     2341 tgatcatgtt cttgatgtaa tcagcgtgac ctggggcatc aatatgtgca tagtgacgct
     2401 tttctgtttc gtattcaatg tgtgaaatat tgatggttac cccacgagct gcctcttctg
     2461 gtgcattatc aatatcggca tatttcattg ccttattgat tgccgaaggc atctttgccg
     2521 ataatgttgt agtgattgcg gctgtcaatg tggttttacc atgatcaacg tgaccaatag
     2581 ttccaatatt gacgtgtggt ttgttgcgaa caaattttga tgcatctgcc atactgctcc
     2641 ttatatatat aaacttactg ttttactact aattacttat tactattatc tactacgttc
     2701 ttcgattatt ttttctgtga tattctttgg cacaatatca taatggctag gcaacatcac
     2761 tgaagatgcc ctgccctgcg acatgctgcg caaaattgta acatatcctt gcatctcagc
     2821 caatggggca agtgcagtaa taattacagt attacctctc gtacttgtac cctgaatttg
     2881 ggctctgcga gaacctaggt caccaatgat atctcccata aactcatcgg gggtcgagac
     2941 ttctagcttc atcactggtt cgagcaacac catctgagct tgttttgttg cttcgcgaat
     3001 tgccaatgaa cctgccattt tgaatgataa ttcactcgaa tctacttcgt gatacgaacc
     3061 gtcatacaga gtaactttca aatcagaaag cgggaagtgt gccaaaaagc cacgtgacaa
     3121 tgattcctgg atacccttat tagttggttc aatatattct ctaggtacta ctccgcccgt
     3181 aattccatta acaaattcgt agccagtgcc tcgctctttc ggttcaatct taatcattac
     3241 gtggccatat tgacctcttc caccagactg cttgatgtac ttaccctcac cttcagctgc
     3301 ttgagtgata gtttctcgat aagcgacttg tggcttaccg acaattgcat ctaccttaaa
     3361 ttctcgtttc attctatcca caatgatttc caaatgcagc tcacccatac cgccaattac
     3421 cgtttggcca gtttcaggat ttgatttgat ttcaaacgtt gggtcctctt cggttaattt
     3481 tgccaacgcc attcccattt tttcttgatc tgacttggtt tttggctcaa tagctaaata
     3541 aatcactggt tcggcaaaag taattccttc taggataatg ggcttgccct catcacaaac
     3601 ggtatcgcca gtcttagtta atttaagacc aacaatcgct acaatttcac cagcgcgagc
     3661 ttcgtctatc tcctcgcgtt gattggcgtg cattaggagc aatcgtccca cgcgttcttt
     3721 ttctctttta gtagtgttgt aggtataaga acccgctttt aatgttccag agtacactcg
     3781 gcagtatgtc agtttaccca catgaggatc ggtttgaatt ttaaaagcca aagcgcagaa
     3841 tggatctgaa ggtaatagtt ttcttacttc cgtttcttgc gtcttaggat tggtaccttc
     3901 aatgtggtca atatctactg gacttggtag atactcaaca acggcatcca ataaaggttg
     3961 aataccggta ttttttaatg atgaaccagc aaaaattggc actagctgat aactaatcac
     4021 tgctttgcgt agagcagcct ttagatcgtt aataggaatt tccttttctg caaaaaattg
     4081 ttcgagtaga gcatcatcgg tttcggcaat cgcttcaacc aaagccgccc gagcagtttc
     4141 cattttttct ttatattgct ccggtacttc cataacttct ggttcggtat ctgttgcaga
     4201 atctttccag aataatgctt tgcgatcgat taattcgatg actccacgaa aatcattttc
     4261 tgctcccatg ggtaacacca tcacagcggg cttgacacca aatttgatac gaatatcctc
     4321 tactgtagca tcgaagtcag cgcccaattt atccattttg ttcacaaaac aaattctcgg
     4381 taccttgtat ttatcggctt ggcgccaaac ggtttctgat tgagactgaa ctccttcgct
     4441 ggcatccaat acagtgatac cgccatcaag cactctgagt gagcgctcta cctctgcggt
     4501 aaaatccacg tgtcctggtg tatcaataat gttgaatcga tattggacct tgggcaaatg
     4561 agaatcaaaa taaggtgtcc aaaaagtagt ggtagcagct gaaacgatgg taattccacg
     4621 ttcttgctct tgttccatcc agtccatggt agctgcacct tcatgaactt cgccaatttt
     4681 gtacgttcgg ccggtataaa acaaaattct ctcagtggta gtcgttttac cagcatcgat
     4741 gtgggcaata atgccaatat tccgaattct ttcgagtgga attttgttac gatctgaact
     4801 agcaaactgc gacataggtt tccttattac ttacttttgc tgtctatcaa caaatcaaaa
     4861 aatagcaaga caaaatgtgc tcactatttt attacttact actactattt taccaacgga
     4921 agtgggcaaa agctttattc gcctcagcca tccgtagcga agtattttta cggtcgatag
     4981 atccaccttg attgttgagc gcatcaaaca tttccgcttc aagtttctct gcgaatgtat
     5041 ggaattgttt gtttggtcgc ttattcgctt cttgtactaa ccagcgtaat gctaatgaca
     5101 ttcctcttct gggtttaacc ggaacaggga tttgatacgc agctccgcca attctacgag
     5161 agcgaacttc cattggtggg gtaataatct gaagcacttc atcaaaagcg gtaagggcat
     5221 cttttttagt tttagtttct agtaattcca aagccttgta tacttgtttt tgagcaacag
     5281 tttttttgcc ttctctcatg acataattga taagcttagt cactcgagta gaaccgtact
     5341 ttgcgtcggg cactactggt aatatttttg cacgttttgt tcgcataatt tctctttact
     5401 taacttttct tcacaccata cttactgcgg gatgtttttc gattctgtac accaatacag
     5461 tcatgttttc cccgaataat atgatatttt acaccaggca aatctttgac tctaccgccc
     5521 ctaaccaaca cgatgctgtg ttctgccaaa gaatgacctt cgcccataat ataggcggta
     5581 atctccgtct tattggataa tctgacacga gctaccttac gcagagctga gtttggtttc
     5641 ttaggcgtca tggttttgac cacggtacac accccacgct tttgtggatt tgtggtagaa
     5701 acaaactgtc cagactttga attataactt ctacgcaaag cactcgcttt tacacgagat
     5761 ggctttctct tgcgaccacc ttttctgatt agttgattaa atgttggcat tatctccttt
     5821 tttgtcgagc gttgattgta tcacatttct aattgagctc tctccttaga tgtgggaatt
     5881 aaacgaccaa taatgacatt ctcctttagt ccttcgagtt tatctacaga accagaggca
     5941 gctgcatcgg ttaacacatt ttttgtgtgc tggaatgatg cggctgatag ccacgaatta
     6001 gtaaataaag atgatcgagt gatacccaag aacatgactt tggcagtggc gggctcgcca
     6061 ccagccgcaa tagtttcctc attatcctca ataaacctac ctcgctcgat aacttcacca
     6121 gtcaagaagt cagtatcacc aattgactct atttggagtt tatcgctcat cttacgaaca
     6181 atcacttcaa aatgtttgtc gttaattgga attccctgag actcgtagac accctgcact
     6241 gcattaatgg catattgttg agctgccaat aatccacgca gtgccaataa ttccttgaca
     6301 ttcattggac catcagtaag tgattgaccc aagtgaacca aatcgccggt cttcactata
     6361 attggtgttt gcaatgggaa ttcatagact gagggctcaa tacttttgtc accaggtgta
     6421 attgtcagca ttaatttacc tgactcttcc tcaattgaaa cctgcccaga aatatctgcc
     6481 ataacagcag cattctttgg cacacgagct tcaaataatt cttccacacg aggcaaacct
     6541 tgtgtaacgt caagtccaac gatacctcca gagtgtttaa tacgcatggt taactgggtg
     6601 cctggctcac caattgattg agctgcaata actccaactg gcacaccgat ttccactctt
     6661 tgacgagttg aaaaatccca accatagcat ttagcgcaca tgccttttgg taaatcacaa
     6721 gaaagaggcg aaaacaccgc aactgaggtg atgtttttac tatcgatcac tgcggcaagc
     6781 tctggtgtaa tcaagtctcc agcagaaaca attgttgttt ttcctgcaat tacatcctgt
     6841 gccgcataac ggccagctat tcgtgatgca aagatatcgt gacgtggttc tttttcaatc
     6901 acgacaaatt tgtctgtgcc acagtcttca gctcgaataa tcatgtcgtg agcaacgtca
     6961 actagccgac gagttaagta accagcgtct gcagttttaa tagcagagtc agtcaatcct
     7021 tttctagaac cgcgagcgga agttacatat tcgaagatcg ataaaccttc tctaaagttg
     7081 gacttagttg gcatttccac aatgttaccc aatggatcta ctaccaaacc tctcatagcc
     7141 gccaactgtt taacctgatc tttagaggca cgtgttcccc cagaattaat cattactttt
     7201 atggcattat cttctgagta tgactgccag gttttatcgg caacttcatc ggtggttttt
     7261 atccaaaggt cgaacgagag acgttttctt tcttcatcag aaattaaacc ctcctgatat
     7321 tggctctgta tcacttcggc ttttttattt gccacttcaa tcatttcatt cttttctgga
     7381 atcatttcac aatcactcac tgagacggat agaccagata gcgtagcaga tgtgaaacca
     7441 aggttcttaa tactatcaat cagtagggtg actacctgcc aatcttcttt ttctatcgct
     7501 tgggtcacaa tacttttaat cattcccgca gtgactgttt gattgagaaa tggaaaggaa
     7561 gctggaagta aatcattaaa ttgcaaccga ccaatggtgg tggtaatgat ttcactttct
     7621 ccatctgtct gacgtcgaac ccgaattggc tggcgcaaac cgatcatttc tacctcataa
     7681 gcatgatagg cttcatctgg agaagaaaaa actacaccat atgcatcacc ttcgtccatt
     7741 actgtgttgt agtaaactcc catcgccatt tctttatttg gaatagtaat aggctcacca
     7801 tttgctggct taagtaaatt gcgtgtcgac ataattaaat ctcgacattc ttcttgagcc
     7861 tgctgtgtga gtggtacgtg aacagccatt tgatcaccat caaaatctgc attgtaacca
     7921 gcacaaacac aagggtgaag gcgaatagca tttccctcaa ccaaaattgg atagaacgct
     7981 tgcataccca atttgtgcag tgttggcgcg cgattaagta caactgggtg gtttatggta
     8041 acttgttcca aaacatcata gacctctggt tctcttcgat ctatcaagtg acgcgcgctc
     8101 ttcacattgg gagcaattcc cttgacaatt aattctctta aaacatatgg cttgaaaatc
     8161 tcaagagcca tatcttttgg cacaccacac tcattcaact ttagttctgg accaaccacg
     8221 atcacacttc ttccagaata atccacgcgc tttccaagca agttttgtct aaatcgacct
     8281 tgcttaccct tgagcatttc ggaaagtgat ttgagtggtc tgcgtgttgc tctgcgactt
     8341 tggcgagctt gcgagccatc aatcaatgaa tctactgctt cctggagcat gcgtttctcg
     8401 ttccgtaaaa taatttgagg cgctcccaaa tcaataagat gtttcagtcg attgttacga
     8461 ttaatgactc ttcgatataa atcattcaaa tcagatgtgg caaatctacc acctgatagt
     8521 tgaaccattg gtcgtaaatc tggaggaatg acaggaagca cattcaagaa catccaggct
     8581 ggtttgattt gcgctttgcg taaaccctct accactcttt gacgtttggt agccttgata
     8641 cgacgagccc ctttagcttc aatcatttcc tttctgagtt tttctactaa ctcatcaaga
     8701 tttaaagctt caacgagctc caaaatactt tcagccccca taccgactct taaatgagaa
     8761 ctagcgtcgt actcgtcaag cttaaggtat tcatcttcac taagaatcgt acctcgtgta
     8821 attcgcttaa ccatagattt aactgtttgg taaatctctt ctgtttgttc cagatgggtg
     8881 tccagttgct gctttaatgc aattaattta ttctttgcct tgacttctat ttctgctgtt
     8941 ttaagttgaa gagtctcttt gctttttact ttttctttca gctgtttttt atcgtcggtt
     9001 agttgctctt tgactttttc aacttcctct ttaattgatg tttttaaact atcaatcgcc
     9061 acttggagat tctcatccaa ggactgcatc accattgctt tttcttcacc atcaacttct
     9121 agtactacat attttgaaaa gtaaataata ctgttgagat tttttggaga aatatcaagt
     9181 aaaagcgaca gttttgatgg cgcacctttg aaaaaccaaa tgtgagctac tggagcggcg
     9241 agcgaaatat gacccatgcg ttctcggcga actttggact gagtaacttc aacgccacac
     9301 ttatcacaag ttatcccacg ataccgtgct cccttgtatt ttccacaata acattcaaaa
     9361 tctttaattg gaccaaaaat cttttcatcg aagagaccat cactttctgc tttgagcgag
     9421 cggtaattga tggtttctgg ctttcgaatt tcgccgtgcg accatgatct catcacttct
     9481 ggtgaagcaa ttctaatttc taatgcgttg aaatcaatga tgtatctggt tttttggttt
     9541 tgggtgttgg ggttattcat agtgctttct cctcatcaat ctctaattct accggctcta
     9601 aatctcctag atcatcagtc ggttcctcag atatttctag ttctttttct gattcatctt
     9661 ctccgtctgc aagtagttct gcgtctggaa gaatatcctc aacaagcaaa gcttcgggat
     9721 ttggtatctc aacttcaact atttcttctt tcgtttcgtg tggcaccacg tccataccaa
     9781 gtgaatttaa ttctctcatc aacaccctaa aggattctgg tactgttgga gaaggaattg
     9841 gttcaccctt cacaattgct tcaaaagcct tagctctacc tatgacatca tccgacttaa
     9901 tcgttaacat ttcttgcaac gtatgagcgg ctcgatgagc ctcaagtgcc cagacttcca
     9961 tttctccaag acgctgacct cccatttggg cttttccacc caatggctgt tgcgttacga
    10021 gtgagtacgg accaacagat ctggcatgca tcttgtcctc aaccatatga ctgagtttga
    10081 gaatatatcc aatgccaaca gtggttgttt cactaaatgg ctcaccagtc ctaccatcaa
    10141 acaattgtgc tttgccagta acgggtagac cagatcgttc taattcctgc cagatctttg
    10201 attcatcaaa ttgttcaaag gctgggaccg caatactgta cccttgtttt tgtgccggcc
    10261 aggctgcgtg agcttcaagc aactgcccca tgttcatacg agctaacacc gacagtggag
    10321 aaatgataat atcgactgga gtaccatctt caagatgagg catgtcagcc acagggacta
    10381 ttttggaaat aacgccctta ttaccatgac gtcctgccaa cttgtcaccc acggtaattt
    10441 tcctaatttg agctactttc actgtgactt ctttaattgt gccaggatca agctcatcgc
    10501 ccatatcgct gtctagaatt tgcacatcaa tgacagtgcc ttgttcaccg tgaggcatac
    10561 gtaaagaagt gtcgcgaact tcgcgagatt tctcaccaaa gattgctctc agcaatcttt
    10621 cttctggtga aagttcggtt tctcccttag gagcaatttt tccaactaat atgtcaccag
    10681 aatcaaccac gctacccact cgaataattc cctctgtagt taaattactc aagtacacat
    10741 cactgacatt gggaatatcg ctagttaatt cttctggccc aagtttggta tccataacct
    10801 tggctcgata ttcgttgatt tgaactgaag tcaagacatc ttcgcgaacc aagcgctcgg
    10861 atataacaat ggcatcttca taacctagac catcaaatga tgcgtacgaa atcagtacgt
    10921 tagcaccaag cgctaattct ccttgatctg cacagggacc atcaatcacc acatcgccaa
    10981 cggttatctt gtcaccaaga gataccactg ctttttgggt gtaacttgta ctttgagacg
    11041 ttctttcgaa tttggaaata tggtaaattt ctcgatcatc ttgaaattcg atagtttcag
    11101 ttgcagtaat agcaggatta atttttgcca ataccgattt gtttaattta acctcaactc
    11161 gatcaccatc cacatataca acttcaccag gatgacgagc taagaccgta cgacccatgg
    11221 cagtagctac tgccatttcc attccagttc caacaatagg tgcctgtggc ttaattaatg
    11281 gcacagcttg acattgcata tgcgttccca tcagggcacg attagcttcg tcatgagcaa
    11341 taaatggaat taatgatgcc gaagtaccaa ccacttgctg tggcagtaca tcaatgaatt
    11401 gcacttcctc taccggtcct tcaatgaatt tattcatagt gcgcatagcg acccattcat
    11461 cagtgatata tcccttttca tcgatatcta tgccggcgtg agtaatcttg taatcttcat
    11521 cttcgtctgc ggcaagatac acgatctcat cactgacttt catcttgccc ttatcagcta
    11581 ccacttttag atagggtgct tctaaaaatc catgcttgtt gactttggca tacaaggcca
    11641 aataggttac caagccaata tttggacctt ctggcgatct aactgggtcg atacgagagt
    11701 attgcgaagc attaatatca cgcatcgaga aagatgcgcg ctctcgggtg acgccaccag
    11761 tacccattac agacaaacgt ctcaggttgt ctatttctga aagtggattt gtttgatcaa
    11821 ggatagttga taagcgattt cgacggaaga attctgaaat agtggcaata agtggacgag
    11881 cattaactaa tgccgctggt gaaagaacat cctctgtctt agttagagac attttttctc
    11941 gaat
//
LOCUS       DAOQZP010000030        11492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_6081_length_11492_cov_5.798461, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000030 DAOQZP010000000
VERSION     DAOQZP010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 11492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11492
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6081_length_11492_cov_5.798461"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..361
                     /locus_tag="PKH60_03000"
     CDS             98..361
                     /locus_tag="PKH60_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17047.1"
                     /translation="MQRGIVTIQPKLVQIIQSHTAEIAEHHPHTWEKLSDAATQFSEL
                     TSIELDKEEVEMILDLLPTPSKDETTELKLARSTIMSLLQSMA"
     gene            complement(365..2050)
                     /gene="gpmI"
                     /locus_tag="PKH60_03005"
     CDS             complement(365..2050)
                     /gene="gpmI"
                     /locus_tag="PKH60_03005"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNQ17048.1"
                     /translation="MQAKITRHAFNDSIQRQPSKAGGPVVLLILDGWGIGPEYPGNAI
                     KLANTNTMDQLWISYPHTQLVASGQAVGLPEGVDGNSETGHMNIGAGSIIFQDLPRIN
                     AAIADGTFAHNQAIQNAINHAKTNNSTLHLFGLVGGGFVHSNVEHLYALLRVAKAAEL
                     QKVMIHAFTDGRDSPPTAGTNYIRRLLDECQSIGVGILASIMGRFYAMDRDKKWDRIE
                     KAYDSLTLGSGRCTKDPVGALQQQYQEGITDEYIEPVNVCEEDGTPRVVQDNDAVIFF
                     NYRVDRPRELTRAFVMQDFEQGFADEDYDPHYEKYHKTSIQEQQFVNTFTRKKVIKNL
                     YFTTMTSYERNLPVDVGFPKLQIKENVGRVLAMHGLRQLRITETEKERMVTYYMNGQS
                     QDGNPGEDWVIFPSKGARSYAEVPEMSALEITEYLVESIGKNEYDVIISNICNGDMVG
                     HTGNLEAGIKACEVVDQMVKRIVDAVLTKNGTVLITADHGNVEEMINLETGEPDTEHS
                     TFPVPFIIVNNDYRGKARMLPSGILADIVPTILHILKEQKPDGMTGRNLFVME"
     gene            complement(2081..2896)
                     /locus_tag="PKH60_03010"
     CDS             complement(2081..2896)
                     /locus_tag="PKH60_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNQ17049.1"
                     /translation="MRKRTRLTITLPNELLKRVDTLVDGSIIRNRSHAVEKILSEGLS
                     PAVSQAVILAGGKSAKRQSLLSLINGQHLLQIVLGQLKTHGILECIICTDHDFIAELQ
                     KRFGDGKELGCQITYMPEVESLGTAGALRNCAHLLNQAPFLVLHGDVLTSINISEFIQ
                     FHLNEQKLATIGVKPRLGERSFGQVFLQGNSIIKFLERGTDQGISIVNTGLYVLDPSV
                     IDMIPKRKPVFLESDVFPALAKQGQLSAFIFQGLWYDITSPSLLEEASRRWLQ"
     gene            complement(2981..3604)
                     /locus_tag="PKH60_03015"
     CDS             complement(2981..3604)
                     /locus_tag="PKH60_03015"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HNQ17050.1"
                     /translation="MQKLILTSSSSQTIHSWVTQLDKSPTDLSLVFIDTAQELEKGDK
                     HWLDEDRQALIDVGFTVLDYTLTGKTQNQLEKDLAPFDVFFVAGGNTFYLLYQAQQSG
                     FLQLISNPDNQKTYVGSSAGSILVCPDIDMIQFIDDPAQAPNLRSTVGANLVNYLVLP
                     HWGNPVFQAGYAKSLEKLYQSKYPAVLLNDNQYLVVEKGYAKLVSNQ"
     gene            3647..4048
                     /locus_tag="PKH60_03020"
     CDS             3647..4048
                     /locus_tag="PKH60_03020"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNQ17051.1"
                     /translation="MSLNVTLRKLLQSFVYAGQGIPVAFRGRNMRVHGAATIAVVGTG
                     LWLGITRFEWLILLMFIAAVWSLEMVNTALEELANTMRDTNHLNYKATKTARDVAAGA
                     VLVMAIIAAISAGIIFIPYLFPPMMYGPPAY"
     gene            complement(4042..4611)
                     /locus_tag="PKH60_03025"
     CDS             complement(4042..4611)
                     /locus_tag="PKH60_03025"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNQ17052.1"
                     /translation="MKTIHFIRLLSIISLLLLAVIFLPQFIAEVQLPGLSPQTNQLVS
                     IAPEEITAVSLISPSNEIQNLSKNGENWQINGVDADVNMVQKLLNSLTDARINSVAAT
                     SEANYEQLGVSSGSAQLVVITSGAIDKTIAVGKPGSTLNTFYAKDADKSEVYLIKGSL
                     LNILSQPIESYIASSSAAASAAATLAPAQ"
     gene            complement(4608..6854)
                     /locus_tag="PKH60_03030"
     CDS             complement(4608..6854)
                     /locus_tag="PKH60_03030"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNQ17053.1"
                     /translation="MKQLFSIIRHEWLRQRVNPATYLLLVIAAGIWYFLFFRQFFLIG
                     QASLRPLFDLYPWFAIVFLPAVTMGSLAKEKTEQTLELLLTHPLREIEVIVAKFLSLL
                     TIPVALSLTTIPLAIGVSIVGQLDWGIYLVQLLATWLFSGFLISFAMVFSALLRDQVA
                     TLLSTIVSSFMWLLLGFELITSRLPIQLSNIVSNLAFLTHLNSLYRGVIDVRDIWFFI
                     SSMVVFLAVAVLYLQRQKHGARKSGYAMSQQVVILLIIVAVLSNIVGQKIPGRIDVTQ
                     SRLYSLTTSTKQVLKQVPDIVTLTLYASNSVPVQLQPILRDTKDILADYQRLGGARIQ
                     VLQKDPSLDAEVKQEAVELGVQEVQFNMISQEEFNVKSGYFGLVITYADQQQTIPFIQ
                     SSEDLEYQLTSLIYQMTRQDKPSVVFAAGHGEKNIQGELAAWAQLLQRQFSLSEVNLT
                     VDEASVAEEIDASAKVLVVAAPASNYEPALVDSLRQFVTNGGSLLVLASGVLADPETQ
                     TAFPVEHNLNTLLEAFGVKIAADFVYDLRSNETIRVSQGTMQYLLPYPLWVRARTTEV
                     NPLIINPQAVMVTWGSSVTAQQETLKEKGWILDPLILTSQYGGVVSNPQSLAPNQEFS
                     ETNLATQTLGVALTPPEGSEYGKIVVIGSGDLLINELIQASPGNSSVGLAAVSWLAGD
                     TMLNTLRSKQAIDRSLVFSSQSQPLVLSVLVYGIAIGVPLIVSLVIFWRRRSQKKLQY
                     QASTLFKL"
     gene            complement(6851..7813)
                     /locus_tag="PKH60_03035"
     CDS             complement(6851..7813)
                     /locus_tag="PKH60_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNQ17054.1"
                     /translation="MSTRVIFGTHCTIMLVLDGVTKRFGSKVAVNQLSLEIKTGQVLG
                     LLGPNGAGKTTTMRLLVGYLSPDSGLITFNGVSVIENPNQVRSQIGYLPENNPLYSEL
                     LVSEMLASSAQLKQMSSASLAKSLDFVVPAVGLEEVYQQPIRELSKGYKQRVGIALAL
                     LHEPEVLILDEPTEGLDPNQRAEIRALLRRLAKDKTVIISTHVMQEVSAICDRIVIIN
                     HGSIVADGTEKTLLQKNNTGTIRAELEGENALSILKKESKLAVLTHKTVGKRLFVELQ
                     PKTKVPAQVVLSEIQRKHQLVLWSLSSVGADLEHLFKTVTEEKS"
     gene            7885..8931
                     /locus_tag="PKH60_03040"
     CDS             7885..8931
                     /locus_tag="PKH60_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNQ17055.1"
                     /translation="MKSIAIFCLLFFIGFSTSFFIITFQSYRSYTNQIESDIVVLQEQ
                     VASNFISSSQDVKKATELPELAQVIQSNTASNRQNLAKAFEILMKKNRSYAQLRFLNS
                     RGDEIVRVNQNQKGITVVPQNELQNKAERYYFTDTMKLNQGEYYISPLDLNIEGSQVV
                     EPYEPTLRLATPVFDASDKKVGMVILNFDTTNVLYELERKDDQNPDYKIYFANSDGEW
                     FRGPTPEDDWSFMFSDGDNRRVKNQYPQLWSNILTAESGSVFAPEGYFYFGKIKPIEL
                     VQQNESIKKTFSYFSRYLEVRDYYMIVIVHMPKTLYGQLLLKKVVGINVFYFLLAGGG
                     LGLAGVMLAKRSSK"
     gene            8962..10113
                     /locus_tag="PKH60_03045"
     CDS             8962..10113
                     /locus_tag="PKH60_03045"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNQ17056.1"
                     /translation="MKTQQKILVVGVCLGLLVSGILITWLSISKITFPAVGIQNTPTP
                     SPQPSPTPSPTPDPLRPRTALLLGYVGGNHEGGLLTDTMLLAQANFKTKELSLISIPR
                     DLWVTIPLQTSQAATSAGKVNQLYALGAEGGLVITQDVVGTVLQQQIEYVLAINQAGL
                     IATLTQLGPIPVKVPYSFVDEYFPIAGEENNPCDKTPEDIATLSATLRGFELEKQFTC
                     RYERLEFTAGLQLLDASTAAKFVRSRHAAIGGSDFGRSQRQQALVLGIKNLLSQPSNW
                     LKIPTVLNKVYRNVDSNLELTDFLELLGLISNPESWKVQSYQLDKTNALWETRSPDGQ
                     YILVDRAASGSAQLNNESWPTLREFVKGWLTQTPAEVASRGGSLDDQAP"
     gene            complement(10153..10851)
                     /locus_tag="PKH60_03050"
     CDS             complement(10153..10851)
                     /locus_tag="PKH60_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17057.1"
                     /translation="MKKNLKPNTFDCSQNVMEKIHTEHIHMKPSWLFYIGSATVIFGI
                     STFMILASILFSLIWYQSLLMGFSQLIRFGSDGWLIFMTRLPWFAVGASFLVALIGWW
                     LVQHSEYGFHYRKRVVGSAIFTAIIVSSLLLHRTNLWDRILRDSTWERVGRPPAVEEE
                     QVLAGRLLRKNQDLYQLLHTSNQVIPIRFAQLQQNTPTAYIPMIGDNIIILGEWKDGI
                     FEVRRFQIWLKETE"
     gene            complement(10835..11431)
                     /locus_tag="PKH60_03055"
     CDS             complement(10835..11431)
                     /locus_tag="PKH60_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNQ17058.1"
                     /translation="MRGLSVKVLSDEELVEKVRKNNQELYVEVVRRYQQPLFRYAFTL
                     VHDSDIAQDIVQEALIKAYTNLRSFNTKKKFSSWIYRITHNQAMDHFRSQRKLVSFEL
                     LPELVSSLVSKSDVEVTLDETLLREKIDHVIDQLPVQYRSVVALYYLEGKKYAEIGEV
                     MKVPEGTVATWLRRSKIILAKALQEYEPKQKKRHEKKS"
BASE COUNT         3415 a         2525 c         2202 g         3350 t
ORIGIN      
        1 atcaccaagc cagtatcaga acatattgca gtccaatatg taggcgaccc atacaatacc
       61 ttgattttgg cttgtttcta gtttaggatg cctaactatg caacgcggta tagttactat
      121 ccaaccaaaa ctcgttcaaa taattcaatc acatactgca gaaattgctg aacatcaccc
      181 acatacatgg gaaaaattgt ccgatgcggc cactcaattc agcgaactaa cctcaattga
      241 acttgataaa gaagaagtcg aaatgattct tgatctcctt cccacacctt ccaaagacga
      301 aacgactgaa ctcaagctag ctcgatcaac gattatgtcg ttgttacaat caatggcgta
      361 aaaactattc cattacaaac agatttctac ccgtcatacc atctggtttt tgttctttca
      421 aaatatgaag tattgtcgga acaatatcag ccaagattcc agatggcaac attcttgcct
      481 tgcctcgata atcattgttc acaataataa agggcacggg aaaagttgag tgctcggtat
      541 ctggctcacc agtttccaga ttaatcattt cttccacgtt gccatggtca gcggtaatta
      601 atactgtgcc attttttgtt agcactgcat caacaattcg tttgaccatc tgatctacaa
      661 cctcacaagc tttaatacca gcctccaaat tccccgtgtg tcccaccata tcaccattac
      721 aaatattact gataattacg tcatattcat ttttgccgat tgattcaact aaatactcag
      781 taatttctaa tgcggacatc tcaggtacct ctgcatatga ccgagcaccc tttgatggga
      841 aaatgaccca atcttctcct ggattaccat cttgcgactg accattcatg taataggtta
      901 ccatacgctc tttttctgtc tcagtgattc tgagttggcg taaaccatgc atagccaaaa
      961 ctctgcccac attttctttt atttgcaact tagggaaacc tacatcaact ggtaaatttc
     1021 gctcgtatga agtcattgta gtgaaataca aattcttgat tactttttta cgcgtaaatg
     1081 tgttgacaaa ttgttgttcc tgaatactag tcttgtgata tttttcgtaa tgaggatcat
     1141 agtcttcatc tgcgaaacct tgttcaaagt cttgcattac aaacgcacga gtgagttctc
     1201 gagggcgatc tacccgatag ttaaagaaga taactgcatc attgtcttgc actactcttg
     1261 gtgtaccatc ttcttcacag acgttgactg gctcgatgta ttcatctgta ataccttctt
     1321 ggtactgctg ttggagtgct ccaaccggat cctttgtgca acgaccgctg ccaagagtga
     1381 gtgaatcgta tgctttttct atgcgatccc actttttatc tcgatccatt gcgtaaaatc
     1441 tacccattat tgatgccaat atacccacac caatgctctg acattcatct agtaaacgac
     1501 gaatataatt ggttcccgca gtgggcggtg aatctcgccc atcggtgaag gcatgaatca
     1561 tgactttttg cagttcagcg gcttttgcca cccgcaacaa ggcatataag tgctcaacgt
     1621 ttgagtgaac aaaaccacca ccaaccaaac cgaataaatg caatgtagaa ttattagtct
     1681 tagcgtgatt gatagcattc tgaattgcct gattgtgtgc aaaagttcca tctgcaatcg
     1741 cagcatttat gcggggcaaa tcttgaaaaa tgatactacc agcgccaata ttcatatgac
     1801 cggtttccga gttgccatca acaccctctg gcaaacccac cgcctgccca ctggctacca
     1861 attgtgtgtg aggatacgag atccacaatt gatccatcgt attcgtattt gcaagtttga
     1921 tagcattgcc cggatactca ggaccaattc cccacccatc aagaattaac aacacaactg
     1981 gcccaccagc ttttgatggc tggcgctgaa ttgaatcatt aaaagcatgg cgagtaattt
     2041 ttgcttgcat agatagcact tactataccg caacgagcgg ttactgcaac cacctgcgcg
     2101 aagcctcttc caacaacgaa ggtgatgtaa tgtcgtacca aaggccctga aagatgaagg
     2161 cgctcaattg gccttgcttg gctaatgctg gaaacacatc gctttctaaa aatactggtt
     2221 ttcgttttgg tatcatgtct attacgctgg gatctaatac atacaaacca gtattaacga
     2281 tgctaattcc ttggtccgtc cctctttcga ggaacttgat aatactgttt ccttgcagaa
     2341 acacttggcc aaatgagcgc tcacccaaac gaggttttac cccaatggtg gcgagctttt
     2401 gttcgtttaa atgaaattga ataaattcag aaatatttat ggaagtcaaa acgtccccat
     2461 gcagaaccaa aaatggagct tggttgagta ggtgggcaca atttcgcaac gccccagctg
     2521 tacccaaaga ttcaacttcc ggcatatagg taatctgaca accaagttct ttaccatctc
     2581 caaaccgttt ttgaagctcg gcaataaaat catgatcagt acaaataata cattcgagaa
     2641 tgccatgagt tttgagctga cctaaaacta tttgtaacaa atgttgacca ttaattaaac
     2701 tcaaaagtga ttgtcgtttt gccgatttac ctcctgcaag aattaccgct tgagaaacag
     2761 ctggtgataa accttcgctc agaatttttt ccactgcatg ggagcgatta cgaataatgg
     2821 atccgtccac tagggtatcg acacgcttta acaattcatt gggaagcgtg atagtaagtc
     2881 tagttctttt gcgcatatgt ttcactcact cctaattatg tatgaaatta tcatacagcg
     2941 tcataccgat tcatacaaat tgaaaaaaca ttagcgagtc ttactgattg gaaactaatt
     3001 ttgcgtagcc cttttcaacc acaagatatt gattgtcatt caataatact gcgggatatt
     3061 ttgattggta cagtttttcc aaagattttg catatccggc ttgaaaaacc gggttgcccc
     3121 aatggggcaa cacaagatag ttaactaagt ttgcgccaac tgtggaccta agattggggg
     3181 cttgcgctgg atcatcaatg aattgaatca tatctatatc tggacacact agaattgatc
     3241 cagcgctgga tcccacatag gttttttgat tatcgggatt tgaaatcagt tgcaaaaaac
     3301 ccgattgctg agcttggtaa agcaaataaa acgtattgcc tccggccaca aaaaaaacat
     3361 caaatggcgc gagatctttc tctaattggt tctgagtttt gccggtcaat gtgtaatcta
     3421 agactgtaaa accaacatcg ataagtgctt gtcgatcctc atcaagccag tgcttatctc
     3481 ccttttctag ctcttgcgct gtgtcgataa aaaccaatga taaatctgtt ggagatttgt
     3541 cgagctgtgt cacccatgaa tgtatagttt gacttgaact cgatgtaagt attaattttt
     3601 gcatgatgct cttgtatttt aacagaatct ttcgtaggct gagtatatgt ctctcaacgt
     3661 cacacttcgt aagttacttc aaagttttgt ctatgctggc caaggaattc ccgtcgcatt
     3721 taggggccgc aacatgcgag tgcatggagc ggcaaccatt gcggtagtgg ggacaggatt
     3781 gtggctggga attactcgat ttgagtggct gatactatta atgtttatcg ccgccgtttg
     3841 gtcgctcgag atggtaaata cagctttgga agagctggcc aatacgatgc gtgataccaa
     3901 tcatctcaac tataaagcaa cgaagaccgc tcgggatgtg gccgcaggag ctgtactcgt
     3961 catggcaata atcgctgcaa ttagtgctgg aattattttt attccctatc tatttccacc
     4021 catgatgtat ggccctccag cctattgagc tggagcaagt gtggcggcgg cggaagccgc
     4081 cgcactgcta gatgcaatat agctttcaat tggctggctc aatatattta atagcgaacc
     4141 tttaatcaaa tacacctctg atttatctgc atctttggca taaaaagtat taagagttga
     4201 tcctggtttg cctaccgcaa tagtcttgtc tattgcccca ctggtaataa ctacaagttg
     4261 ggctgagcct gaactaactc caagctgttc ataattcgcc tcactggttg cggcaacaga
     4321 gttaatccgc gcatccgtga gtgaatttag aagtttttgt accatattta catccgcatc
     4381 cacaccattt atttgccaat tttcaccatt tttagataaa ttttgaatct cgtttgaagg
     4441 actgatgagt gaaactgctg tgatttcttc tggagcgata gaaactagtt ggtttgtttg
     4501 gggtgataat ccgggtaatt gaacttcggc aataaattgc ggtaagaaaa tcactgccaa
     4561 taacagaagt gaaattattg agagtaaacg aataaagtgg atggtcttca tagtttaaat
     4621 agtgttgatg cttgatactg aagttttttt tgtgatctac gacgccaaaa gatcaccaga
     4681 cttacaataa gtggaacgcc tatggctatg ccatacacta aaacagataa aactagtggc
     4741 tggctttgtg aagaaaaaac caaagagcgg tcaatcgctt gtttactccg taaggtattc
     4801 agcatcgtat caccagccaa ccaagacact gctgccaaac ccacggaact attgccgggt
     4861 gaggcttgga tcaactcatt aattaataaa tcaccagatc caattaccac aatttttccg
     4921 tattcactac cctctggtgg ggtaagcgcc accccaagtg tttgtgttgc caaattcgtc
     4981 tctgaaaact cttggtttgg cgctaacgac tgaggattcg ataccactcc accatattgg
     5041 cttgtaagaa taaggggatc tagtatccaa cctttttctt tgagcgtctc ctgctgagca
     5101 gtcactgagc ttccccaagt gaccatcacc gcttgaggat tgatgatgag aggattgact
     5161 tctgttgtgc gcgctcgaac ccaaagtgga tacggtaata aatactgcat tgttccctga
     5221 gaaacccgta ttgtttcgtt cgagcgcaaa tcgtacacaa aatctgctgc gattttcaca
     5281 ccaaacgctt caagcaacgt gttgaggtta tgttcaactg gaaaagcagt ttgggtttct
     5341 ggatcagcta aaacccccga ggctaacacc agtagagagc caccgttcgt aacaaactga
     5401 cgcaaagaat caactagtgc gggttcatag ttactcgccg gtgccgctac tactaatact
     5461 tttgcggaag catcaatttc ttctgcgaca cttgcctcat caactgttaa gttgacttca
     5521 gataaggaaa actgcctctg taataattgc gcccatgcag ctaattcgcc ctgtatattt
     5581 ttctcaccat gaccagctgc aaaaactacc gatggcttat cttgtctcgt catttggtat
     5641 attaagcttg tgagttggta ctctaagtct tccgagcttt gaataaaggg aatcgtttgc
     5701 tgctggtctg cataggtaat tactaaacca aaataaccac tcttaacgtt aaactcttcc
     5761 tgactaatca tattaaactg tacttcttga actcctaact caaccgcctc ttgtttaact
     5821 tcagcatcta gacttgggtc tttttgcagt acctgaattc gcgcaccacc caatcgttga
     5881 taatctgcaa gaatatcctt ggtatcccta agaattggct gcaattgaac tggcactgaa
     5941 tttgaagcgt agagcgtgag cgtcactata tcaggaacct gtttgagtac ttgctttgtt
     6001 gaggtggtca aactgtaaag ccgtgattgt gtcacgtcaa ttcttcccgg aattttttgg
     6061 ccaacaatat tgctaagtac agctactata atcaacaaaa taaccacttg ctgtgacatg
     6121 gcgtaccctg attttcttgc cccatgtttt tgacgctgca gatataagac ggctacagca
     6181 agaaatacaa ccatacttga gataaagaac catatatcac gaacatcaat caccccacga
     6241 tacagtgagt ttaagtgagt taaaaaagcc aaattactaa caatgttgct taattggatt
     6301 ggtaaacgac tggtaattaa ctcgaaccca agtaatagcc acataaaact agatacaatt
     6361 gttgatagta gggttgccac ttggtctctc agtagtgcgg aaaaaaccat cgcaaaagaa
     6421 attagaaaac cagaaaaaag ccaagtggct aataattgca ctaaatagat tccccaatca
     6481 agctgcccaa caatgctaac cccaattgca agtggaatag tggttaaact gagtgcaaca
     6541 gggatagtaa gcaaacttag aaatttggcc acgatcactt caatttctct caaaggatgt
     6601 gtaagaagta attccaatgt ttgctcagtt ttttccttgg ccaagctacc catggttact
     6661 gcgggtaaaa aaactatggc aaaccaagga tacaaatcaa ataatggcct gagcgatgct
     6721 tggccaatta agaaaaattg acgaaaaaag agaaaatacc aaattcccgc agcaatcaca
     6781 agcaacaaat atgtcgctgg attgacacgt tgtctgagcc attcatgacg aataattgaa
     6841 aacaattgct tcatgatttt tcctccgtca cggttttaaa caagtgttcc aaatcagctc
     6901 ctactgagct gagagaccaa agcacaagtt ggtgctttcg ctgaatttca gacagaacca
     6961 cttgagctgg aacttttgtt tttggttgta actcaacaaa caaacgttta cccactgttt
     7021 tatgagtcag cactgcaagt tttgactctt tttttaatat cgataacgca ttctcacctt
     7081 ctagttcggc acgaatcgta ccggtattat ttttctgtaa taaggttttc tctgttccat
     7141 ccgcaacaat cgaaccatga ttaattatta caattcgatc acagatggcg ctcacttctt
     7201 gcatcacatg agtactaata atcactgttt tatctttagc taatcttcgt aagagcgctc
     7261 gaatttctgc gcgttggttg ggatccaaac cttcagttgg ctcatcaaga atcaatacct
     7321 ccggctcatg caataaagcg agtgcaatac caacgcgctg tttgtaaccc ttgctgagct
     7381 ctctgattgg ctgttggtat acctcttcca aaccaacagc gggcacaaca aaatcaagtg
     7441 attttgccag cgatgctgaa ctcatttgct tgagctgagc actgcttgct aacatttcag
     7501 atacaagtag ttctgaatag agtgggttgt tttctggcag ataaccgatt tgacttctga
     7561 cttgatttgg attttcaatt acagaaacac cgttgaaagt aattaatccc gaatctggac
     7621 tcaggtagcc aacgagcaac cgcatagtgg ttgttttacc ggcgccattg ggaccaagaa
     7681 gaccaagaac ttgaccagtt tttatctcga gagacagctg gtttaccgcc acctttgaac
     7741 cgaatcgctt ggtaactcca tcgagcacta acatgatggt gcaatgtgta ccaaaaataa
     7801 ctctagttga caagcactaa tattaattgc acattaaata ctcatctgaa cttgctatgt
     7861 ttacttgtta caattaatct cttgatgaaa tctatcgcaa tcttctgcct actatttttc
     7921 attggttttt ccacaagctt ttttataatc acatttcaat cataccgaag ctatactaat
     7981 caaatcgagt cagatattgt ggtgctgcag gaacaagtag caagtaactt tataagtagc
     8041 tcgcaggatg taaaaaaagc gactgaatta ccagaactcg cgcaagttat ccaatctaac
     8101 actgcctcta atagacaaaa cctagcaaaa gcctttgaaa ttttaatgaa aaaaaatcgc
     8161 tcatatgctc aattgagatt tcttaatagt agaggagatg aaattgtcag agttaatcaa
     8221 aaccaaaaag gcattaccgt tgttccacaa aatgaattac aaaacaaagc tgaacgatac
     8281 tatttcacgg atactatgaa gctgaaccag ggtgagtatt atatctcccc acttgacctt
     8341 aacatcgaag gctcccaagt cgttgaacca tatgaaccaa ctcttcgcct agctactccc
     8401 gtctttgatg cttcagacaa aaaagtaggc atggtaattc tcaactttga tacaaccaat
     8461 gtactgtacg aacttgaaag aaaagatgat caaaatcctg actataaaat ttattttgct
     8521 aactctgatg gcgaatggtt ccgaggtcca actcctgagg atgattggtc atttatgttc
     8581 tcggacggag acaatcgtcg tgtaaaaaac caatatcctc aactctggtc aaatatatta
     8641 actgcggagt caggctcggt gttcgcacca gaaggatatt tctattttgg gaaaatcaaa
     8701 ccaattgaat tggtgcaaca aaatgaaagt ataaaaaaaa ctttttccta tttttcccgg
     8761 tatttagaag ttagagacta ttatatgatt gtgatcgttc atatgccaaa aactttatat
     8821 ggccaactgc tactcaaaaa agttgttgga ataaatgttt tctatttctt gctggccggt
     8881 ggagggttgg gactggcggg ggtcatgctg gcaaaaagat cgtcaaaata aactagtcat
     8941 ttcaatgaga taatgcgttt gatgaaaaca cagcaaaaaa tactggtggt tggtgtttgc
     9001 cttggactgt tggtcagtgg catactgata acgtggttga gtatttctaa aattactttt
     9061 ccggctgttg gtatacaaaa tactccaaca ccttctcccc aaccttcccc aactccctct
     9121 cctacacccg atccattacg accaagaaca gctctactgt taggatatgt ggggggaaat
     9181 cacgaaggtg gtttgcttac ggatacgatg ttactagcac aagcaaattt taaaacgaaa
     9241 gagttatcgc taatttcaat accaagagat ctttgggtca cgatacctct tcaaacctca
     9301 caggcagcca catcagcggg gaaagttaat caattgtatg ctctaggcgc tgaaggtgga
     9361 ttagtcataa cccaagacgt tgttggcacc gtactccaac aacaaattga gtatgtattg
     9421 gcaattaatc aagctggatt aattgccact cttacccaac ttggtccaat tcctgttaag
     9481 gtaccctaca gttttgttga tgagtacttt cccatcgccg gcgaagaaaa taatccttgt
     9541 gataaaactc ctgaagatat tgctacgctg tcagccacac tgcgtggatt tgaactagaa
     9601 aaacaattta cttgcagata tgagcgtttg gaatttactg cgggcttaca acttctcgat
     9661 gcatctactg ccgccaaatt cgtgcgctcc cgtcacgcgg ctattggcgg cagtgacttt
     9721 ggccgctccc aacgtcaaca agccttagtt ttaggaatta aaaatctttt gagtcaacct
     9781 tcaaattggt taaaaattcc tacagtgctg aacaaagtat atcgaaacgt ggatagcaat
     9841 ttggagctga ctgatttctt agaactccta ggcttaatct ccaatcccga aagttggaag
     9901 gtacaaagct atcagctgga taaaactaac gcgctatggg aaactagaag tccagacggt
     9961 caatatatac tggtagatcg agctgcttct ggcagcgcac aactcaacaa cgaatcctgg
    10021 cctacattga gagaattcgt gaaagggtgg ttaacacaaa cccccgctga ggttgcctca
    10081 cgcgggggtt ctcttgatga tcaggcgcct tgactttcct gtttccaccc atctaactgt
    10141 tatacgcatc gattattcag tttctttcaa ccaaatttgg aaacgtctca cttcaaaaat
    10201 tccatctttc cattctccta aaattattat gttgtcacca atcataggta tataagccgt
    10261 gggtgtattc tgttgtaact gagcaaatct aattggtata acctgattgc tagtatgcag
    10321 caattgatat aaatcttgat tttttcttaa taacctaccc gctaatacct gctcctcttc
    10381 taccgctgga gggcgaccaa ctctttccca ggtactatct cttaagattc gatcccaaag
    10441 attagtcctg tgcaacaata aactactcac gatgatagcg gtaaagatgg cagacccaac
    10501 cactctctta cgataatgaa aaccatattc cgaatgctgg accaaccacc agccaatcag
    10561 cgccactaaa aaactggctc cgacagcaaa ccagggcaag cgagtcataa aaatgagcca
    10621 accatccgaa ccaaaccgaa tcaattgtga aaatcccatt agtaatgatt gataccaaat
    10681 cagactaaaa agaatgctgg ctaaaatcat aaaagtacta ataccaaaaa ttactgtggc
    10741 agaaccaatg tagaaaagcc aacttggctt catatgaatg tgctctgtgt ggattttttc
    10801 catcacattc tgactacaat caaaagtgtt gggtttaaga ttttttttca tgtcgttttt
    10861 tttgttttgg ctcatattct tgtagggctt tagctagaat aatttttgaa cgcctcaacc
    10921 aggttgccac ggtgccctca ggtactttca taacctcacc aatttctgca tatttctttc
    10981 cctcaagata gtagagggca actacactcc tatactgtac cggtaattga tcaatcacat
    11041 gatcaatttt ttctcgaagc aatgtttcat ctaaagtcac ctcaacgtca gatttactga
    11101 ccaaagaact aaccaattca ggcaacaatt caaatgaaac aagtttccgt tgacttcgaa
    11161 agtggtccat tgcctggtta tgagtaatac gataaatcca gctagaaaat ttctttttgg
    11221 tgttgaaact gcgtaaatta gtatatgcct taatcaaagc ctcttgcaca atatcttgag
    11281 caatgtctga atcatgcact aaagtaaagg cgtaacggaa caggggttgc tgatagcgtc
    11341 gtacaacttc aacgtagagc tcttggttgt tttttcgaac tttctctacc agctcctcat
    11401 ctgagagcac ttttactgat aatccacgca tgattttgct atactatact gaagggtttt
    11461 gaaatgctac tgaaaaaacc agaaaaattc at
//
LOCUS       DAOQZP010000031        11257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_6224_length_11257_cov_5.737190, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000031 DAOQZP010000000
VERSION     DAOQZP010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 11257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11257
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6224_length_11257_cov_5.737190"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..90)
                     /locus_tag="PKH60_03060"
     tRNA            complement(3..90)
                     /locus_tag="PKH60_03060"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Ser,seq:act)
     gene            complement(99..1175)
                     /locus_tag="PKH60_03065"
     CDS             complement(99..1175)
                     /locus_tag="PKH60_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17059.1"
                     /translation="MFTLQPIKLWRYLSLHRLVIPPTLRRYFYVSWEDALWDLLRNFQ
                     VPRNSVCLVPEFFCMDVVKNMEEHGLQCIFYPVDLQLQTSSTIFSDRLRTHKPKVVVI
                     LHVAGITNQLLSQSNIWLKYLPTNALLIEDCVHRLVDPQQISLLTPRHFVIDSLRKVA
                     PLYGSNLYGDASTLRNLKQSGWWLTPSYQLQVLWWWWLFQISLHLGWNSPAHRLMQKG
                     YDLIGDSNRAAPGPWVFNFLSQYLDIVRVEKVKERQVTLYQELLKPCWTNSNLYQIHF
                     DQADVGKLRGFPIGIVNKNLKQFLQKLQESQLAWKYELDDCPWSKRQKIIYLPLGPHL
                     QLNEVDTICQLFITALKNINSVVS"
     gene            1230..1532
                     /locus_tag="PKH60_03070"
     CDS             1230..1532
                     /locus_tag="PKH60_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNQ17060.1"
                     /translation="MSSYKCCTPNTKEFAHANSLSQLLQLVGETNRLQILCLLRQGTH
                     CVCEIMEHFPMSQSLISHHLADLREAGIVTNVKSGRKVLYSLTKNGKKITEKVFSL"
     gene            1541..1804
                     /locus_tag="PKH60_03075"
     CDS             1541..1804
                     /locus_tag="PKH60_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNQ17061.1"
                     /translation="MKIQVLGSGCTNCKKLYESVAQVAKELQLETEVEYVVGTDGLQK
                     MIELGALGSPVIAVDDAIVMTGFTADKNKIISVLQPKQAQVKL"
     gene            1801..2781
                     /locus_tag="PKH60_03080"
     CDS             1801..2781
                     /locus_tag="PKH60_03080"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNQ17062.1"
                     /translation="MSIFDPIQAFADVITYNWLGFDHHSFIGESLNYFIFDVIKIGLL
                     LILINYLMAFVRYYFPMEKVKTSLTKRRWYGLDYLLAALLGMITPFCSCSSIPLFIGF
                     VGAGVPLGVTFAFLISSPLVNEASLYLFPALFGFKMTLIYNSIGILVAIGGGLLIQRL
                     KFEKYIRPELLQITTRNQTSIKNEVEKLPFRKLAVLCWHDGMHITKQVFPYVLLGVAL
                     GSLIHGFVPAGFVEKYLSMKTWWSVPVATILGAPLYANSVGVMPIMEALVEKGVPLGT
                     ALAFMTSIVTISLPEILILKKVMKWQLLAVFVSITVVGIMLMGYTLNLIG"
     gene            complement(2880..3137)
                     /locus_tag="PKH60_03085"
     CDS             complement(2880..3137)
                     /locus_tag="PKH60_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17063.1"
                     /translation="MTEKLTQIQYKEELLPAVYVRTDVICPGVTCEVYLDPTTGERDY
                     GKVTVEPGYKTPKQRVLSGEETMVPHQPLILILKIRDSLMK"
     gene            3181..5010
                     /locus_tag="PKH60_03090"
     CDS             3181..5010
                     /locus_tag="PKH60_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17064.1"
                     /translation="MKKNKNSLISKSLLLFILLISIGSFLRLWRLGSLPEIMHRDEAA
                     IGYNAFAIVETGRDERGQPWPMNFKSFGDYKLPGMIYTVMATIKFFGLTASAVRLPTA
                     TASVLSLLAIYWLSREMGWSKRTSLIAMGFLTLSFFHIAQARNAYEPMVGLLFSLTGF
                     ASWLAGPRNWRWYLISLLMYLVGSLFYNLPFLLMPILYVISWVITNPPISSRKILTMT
                     TKIWPLVGLVTLVVVLSLLFQSSNVGKSNITIFKSDEIVKMAENSVHATLVGGVPSRI
                     ARLVNSVSLFSVVQFFKGYFEVFNPVYIFFTGDANLWHNLRSIGLGNHNPIMIIPFLM
                     GIYVIIKNGAKKQVKLVAMYLGLSPIISALTINSPVTNRLMDFNTAVVLVAAVGTSEI
                     IVKYWRSNQIFNRLLITSIVTLYCGFFFLFGLRYYFSYNQLLAREWNPGMNEMIRWLK
                     ENRTKYDWIYLTPDFDLAYIHMAFHLPFDPADFQQSAQWYTNGFELVGEYRQFRFDHF
                     LDYSYLSTRNVTKIFDDQHKTILVVQKGEPKVKTNLLWQDQDWGGRWRWYAWETDLDK
                     TIQWLEATASTPDQIKTLRYLYSCRTGSCDKLVLELDPTAVIN"
     gene            complement(5028..5459)
                     /locus_tag="PKH60_03095"
     CDS             complement(5028..5459)
                     /locus_tag="PKH60_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ17065.1"
                     /translation="MIKINKASSSDATQLVAIINSALGYGVSENTLNGRIKNILLRND
                     HLLLVAKIDDQVVGFCHGYLRLLTEVVEAIEIGGLAVVETHRGKGVARQLVTAIEDWA
                     KSLGYTQVVLASNITRTNAHEFYEHLGYKKIKQQFAFEKNL"
     gene            complement(5480..>5893)
                     /locus_tag="PKH60_03100"
     CDS             complement(5480..>5893)
                     /locus_tag="PKH60_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HNQ17066.1"
                     /translation="MTITICGSMSFHQQMKEAQARLEKRGFTILLPEELADGSVNEAL
                     LEKDEDKISAKIEYNFIREHFKKVEMADSILILNYPKKDIDGYIGGNTFLEMGYAFGL
                     GKKIFLLYPIPRMDYFTEMSAIQPIVLNGNLNLLE"
     assembly_gap    5894..5993
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6063..6401)
                     /locus_tag="PKH60_03105"
     CDS             complement(6063..6401)
                     /locus_tag="PKH60_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17067.1"
                     /translation="MTNIQLPNHKYASQHATKLWQALRDSGIDAELEYFDGHKTVDIA
                     ILPSRIYIEVDGLHRFTEAEQIIKDFKRAHYSDGDDFNTFYVTNQIIELYLEDVVKAL
                     VEVVALRMPR"
     gene            complement(6617..6699)
                     /locus_tag="PKH60_03110"
     tRNA            complement(6617..6699)
                     /locus_tag="PKH60_03110"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6663..6665),aa:Ser,seq:tga)
     gene            complement(6731..7579)
                     /locus_tag="PKH60_03115"
     CDS             complement(6731..7579)
                     /locus_tag="PKH60_03115"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNQ17068.1"
                     /translation="MQGKSLGFNINRWFALWFCRPGWQKLFYHGHKLMLRGLGLLNAD
                     GDDVTGENWLLDRLARSDIKTVVDVGANVSIYGYDRLPKRVRFIGFEPNPETFSQLQK
                     KNYPSRVQLHQLAVGATEKTIKLYDFADDAVLKTTQPTATLASTSKAVIEDFHHQKSK
                     AYDVKQIALDDFLKKQKVASIDLLKIDVEGYELSVLQGAKQLIAKQKIDLIQFEFNEM
                     HVYHRVFFKDFINLLPNYTLYRLGPNGLLPLPPYRPLTHEIFAFQNVLAISNQNQARW
                     FNLLNS"
     gene            complement(7610..8332)
                     /locus_tag="PKH60_03120"
     CDS             complement(7610..8332)
                     /locus_tag="PKH60_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ17069.1"
                     /translation="MKKLSSLSVFFPAFNEEENIPHLLEQALEYIPKFANTYELIVVD
                     DGSQDNTKAIAQSQAEMNAHIRVISHATNLGYGAALKTGFEQAKYEWVFFTDADLQFN
                     FSELESFLEFTDLYSAVIGYRKTRAEGFGRARNAYLFKVFINLLFRVHVKDIDCAYKL
                     FKTELIKPLKLQSDGAFISAELLYKLKKNHIKIKQLPVTHYPRLRGNPTGANLKVILK
                     AGIDALSLYFSMKFGRLIKHQW"
     gene            8408..9466
                     /locus_tag="PKH60_03125"
     CDS             8408..9466
                     /locus_tag="PKH60_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNQ17070.1"
                     /translation="MSEKHHHSPYQVGIFFVLFGLIGVLSFIVLQPYLSLVFFSLIYS
                     ILIWPIYQRVSKRLGSRKSLSTLASIVLSILLLVIPGIIFAQTLVIQAVTVVTDLQSQ
                     VGPETLEQVVTQINSSVKVLPFAQDLEVTSQQLVQLIVQNLQPATDWFLSSFWQIGNF
                     SVTFMINLVLLIILVYFILPNLPDLKRYIYKISPLSKDATHQYFSRSYAMLLDVIKGT
                     FLIAILQGLVGGIFLAIIGIPAWVLFAFLMMVLSMIPILGTGFVLVPIGLVLLLTGHP
                     FLGILTMCWQFFVVGTIDNVVRSMVVSKVANVHPALMMVAVLGGIKAFGFLGLVYGPL
                     ILVVFVTTLEVYRQEYHQ"
     gene            complement(9460..10344)
                     /locus_tag="PKH60_03130"
     CDS             complement(9460..10344)
                     /locus_tag="PKH60_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNQ17071.1"
                     /translation="MKITKALITAAGFGTRFLPITKTIQKEMLPFLDRPIIDYVVDDL
                     LAAGVTDIIFVVSEHNKQLIHYYRENQRLYNYLADRNKASLYDQVAKLHQKATFHFIK
                     QSDSEQYGTAVPLKLAQSLLQTDDAFFVFMGDDLMYNSNGNSEAAAMIQLMEKSHAVA
                     VATCVEKPTSELSRYGVIKPKKHGDYVLLESIVEKPQPGSEPSNLVNISKYIFTPQVF
                     EVLANQQLDVNSGELYITDTITQLAQANDVAIHVPTGTYLDGGNPFEWLKANISIGLH
                     REDMAQKLRDFLTETLAH"
     gene            10371..10880
                     /locus_tag="PKH60_03135"
     CDS             10371..10880
                     /locus_tag="PKH60_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNQ17072.1"
                     /translation="MTETKTARETILSTTNFIHCGDDYLFIHRTKKGSSVDFGRLNGI
                     GGKVEPGETFVACAIRETLEETGYEVPQENCQLRAVVNLSGGYAQDWMMCFFSMTVET
                     KNVPLGMENPEGNLLWLPASQVLTSGYELVDDLQYIWTDLVEDSRVLFFAAQLNKQEK
                     ITQHSIFHL"
     gene            10926..>11257
                     /locus_tag="PKH60_03140"
     CDS             10926..>11257
                     /locus_tag="PKH60_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17073.1"
                     /translation="MSGWFNLALVLLAVICLALLGIWQLGYDPIAHLSCVKLEDSYLS
                     IKKTGEKTGRYVCVHTFSDGGKTCVSSNECEGDCMVTEQTKIESDGFYAWSQQFNATD
                     RISGCTILT"
BASE COUNT         3126 a         2104 c         2313 g         3614 t
ORIGIN      
        1 tttggcggag ggagagggat tcgaaccctc ggtagctttc accacggcgg tttagtaaac
       61 cgctgcactc gaccactatg cgatccctcc aagcagtctt atgatactac agagttgatg
      121 ttttttaatg ctgtaataaa tagttggcaa atagtatcca cttcgtttaa ttgtagatgt
      181 ggtccaagcg gaagataaat gattttttgc cgcttggacc acggacaatc atccaactcg
      241 tatttccaag ccagctgact ttcctgtaac ttttgtagga attgtttgag attcttattt
      301 actatgccaa ttggaaaacc gcgtaactta cccacatcag cttgatcgaa atgtatttga
      361 tacaaattgc tattggtcca gcatggtttg agcaattctt ggtaaagagt tacctgtcgt
      421 tctttaactt tttcaactct cactatatct aaatattggg aaaggaagtt aaagacccat
      481 gggccaggtg ccgctcgatt gctatcaccg attagatcgt atcccttttg catgagtcta
      541 tgagcaggag aattccaacc caaatgtaaa gaaatttgaa atagccacca ccaccagaga
      601 acttgaagct gataggaagg tgttagccac catccagact gttttaaatt acgaagggta
      661 ctggcatcgc catacaaatt actaccatag agtggagcta ctttacgcag actatctatc
      721 acaaagtgac ggggtgtgag taaactaatt tgttgtggat caactagtcg atgaacgcag
      781 tcttcgatta gtaatgcatt tgttggtaaa tatttgagcc aaatattgct ctgagagaga
      841 agttgattgg tgatgcctgc gacatgcaaa ataaccacta cctttggttt gtgtgtacgt
      901 agacgatcgg agaagattgt agaacttgtt tgcaactgta aatccactgg ataaaaaatg
      961 cactgcaatc catgttcttc catattcttt accacgtcca tgcaaaaaaa ttctgggact
     1021 aaacaaactg aatttcttgg tacttgaaaa ttccgtaaca agtcccataa cgcatcttcc
     1081 catgacacat aaaaatagcg gcgaagagtg gggggtatta ctaatcgatg gagagataaa
     1141 tatctccaaa gcttaatcgg ttgcagcgta aacatactaa tgcgctagtg tagcattgac
     1201 agcaaatgta tatatgttaa tatatgaata tgagttcata taaatgttgt acaccaaaca
     1261 caaaagaatt tgcccatgca aattctttat cccaactttt acaacttgta ggtgaaacca
     1321 atcgcttgca aattctttgc ttattacggc aaggcacgca ttgtgtctgt gaaattatgg
     1381 aacattttcc gatgtcacaa agcttaattt cacatcactt agctgattta agagaggctg
     1441 gcatcgttac caacgtcaag tcgggtagaa aagttctcta ctcgctcaca aaaaatggaa
     1501 aaaaaattac tgagaaagta ttttctttat gaaaggacaa atgaaaatcc aagtacttgg
     1561 ttcaggttgc acaaactgca aaaaattata cgaatcagtt gctcaagttg ctaaggaact
     1621 acagctagaa actgaggtag agtatgttgt tggaacagat ggtttgcaga aaatgatcga
     1681 gcttggtgct ttaggaagtc cagtaatcgc agtcgatgac gctattgtta tgactggatt
     1741 tacagcagac aaaaataaaa ttatcagcgt acttcagcct aaacaagctc aggttaaatt
     1801 atgagtattt ttgatcctat ccaagctttt gccgacgtaa tcacttataa ctggttgggt
     1861 tttgatcatc actcatttat tggcgagtca ttgaattatt ttatcttcga tgtcattaaa
     1921 atcggtttat tgttaatact aatcaattat ctaatggcgt ttgttcgtta ctatttccct
     1981 atggaaaaag tcaaaactag tttgaccaaa cgacgctggt atggcttaga ttatttactc
     2041 gcagctctgt tgggtatgat tacaccattt tgttcatgtt cctcaatccc actctttatt
     2101 ggtttcgttg gtgcaggcgt gccgttgggt gtgacttttg cttttttaat tagctctcca
     2161 ttagtcaatg aagcttcact ctatttattc cctgcactct ttggcttcaa aatgacgcta
     2221 atttataaca gcattggaat tctggtagcc atcggcggcg gcttgttgat tcaacgtcta
     2281 aaatttgaaa aatatattcg tccagaatta ttacaaataa cgactcgaaa tcaaacaagt
     2341 atcaaaaatg aagtagagaa attaccgttt cgaaaacttg cagttttgtg ttggcacgat
     2401 gggatgcata ttactaaaca agtgtttcct tatgttttgc ttggtgttgc gcttggttcg
     2461 ctaattcatg gttttgtacc agcgggattt gtggaaaaat atctgagcat gaaaacatgg
     2521 tggtcagttc cagttgcaac tatattaggt gccccactct atgcgaattc ggttggagtg
     2581 atgccaatta tggaagcttt ggtagaaaaa ggcgtaccgt tgggcactgc tctagcgttt
     2641 atgacctcaa ttgttaccat ttcattacca gaaatattga ttttgaaaaa agtgatgaag
     2701 tggcaactct tggcagtatt cgttagtatt actgttgtcg gaattatgtt gatgggatat
     2761 accctaaatt taatcggcta attcctcgaa tcgcccatcg gtgaatggag gatagcagat
     2821 ctcaacaaag actagcatct catcgttact ggcttcccat tgcattattt caccaactct
     2881 tacttcatga gcgaatccct gatctttaga atcaagatca aaggttgatg tggcaccatc
     2941 gtttcctcac cactaagcac tctttgttta ggggttttgt aaccaggttc aactgtaact
     3001 ttgccataat ctctttcgcc agttgttgga tcaagatata cttcacatgt tactccagga
     3061 caaataacat cagttctcac atatacagca ggcaataact cttctttgta ctgaatttga
     3121 gtaagttttt cagtcattct atatattcta tcagcaccag tgatacacta agtattgcca
     3181 atgaaaaaaa ataaaaatag tttaatttca aaaagcttgt tgttatttat actactaata
     3241 agtattggtt cgttccttcg tttgtggcgt ttgggttcat tgccagaaat catgcatcgg
     3301 gatgaggcag caattggcta caatgccttt gccatagtgg aaacaggcag agacgaacgt
     3361 ggtcaaccat ggccgatgaa ctttaaatct tttggcgact acaaactgcc cggaatgatc
     3421 tataccgtta tggcgaccat taagtttttt ggtttgaccg cttccgcagt aagattgccc
     3481 accgccaccg catcggtgtt gtcattgtta gctatctact ggttgagtcg agagatgggc
     3541 tggtcgaaac ggacgagtct gattgcgatg gggtttttaa ccttgagctt ttttcatata
     3601 gctcaagcca gaaatgccta tgaaccaatg gttggtctct tgttttcact aactggattt
     3661 gccagttggc tggcgggtcc gagaaattgg cggtggtatc tcatttcatt acttatgtat
     3721 ttagttggtt cactttttta taatctgcca tttttactga tgccaatctt gtatgtgatt
     3781 agctgggtaa tcacgaatcc tccaatttct agcagaaaaa ttttaacaat gactacaaaa
     3841 atatggccat tggttgggct agtcacattg gtggttgtac tcagcttgct ctttcaaagt
     3901 tccaacgttg gtaagagtaa tatcacgata tttaagtctg atgaaattgt taaaatggca
     3961 gagaattcag ttcacgccac cttagttggt ggtgtaccat caagaattgc tcgattggtt
     4021 aacagcgtaa gtcttttttc ggttgttcag ttttttaaag gttactttga ggtgtttaat
     4081 ccggtgtata tttttttcac cggtgatgct aatttatggc ataaccttcg atcaattggt
     4141 ttaggtaatc acaatccaat catgattatt ccatttttga tgggtattta cgtgataata
     4201 aaaaatggcg ccaaaaagca agtgaagcta gtagcaatgt atttaggttt aagcccaatt
     4261 attagcgcgt tgactataaa ctcgcccgtc acaaaccgac tgatggattt taatactgcg
     4321 gtggttttag tggcagcggt aggcactagt gaaataattg tgaaatattg gcgctctaat
     4381 caaatattca accgtctact tataactagc attgtcactc tctattgtgg ctttttcttt
     4441 ttgtttggac tgcgctacta tttttcctat aatcagttgt tagccagaga atggaatccg
     4501 gggatgaatg agatgatacg atggctaaag gaaaatcgca ctaaatacga ctggatttat
     4561 ttaactccag attttgattt ggcatatatc cacatggctt ttcacttacc atttgatcca
     4621 gctgattttc aacaaagtgc tcaatggtac accaatggct ttgagttagt tggggagtac
     4681 cgtcagtttc gatttgacca ttttttggat tatagttatt tatcgactcg gaacgtgacg
     4741 aaaatattcg atgatcaaca taaaactatt ttagttgtgc aaaaaggtga accaaaagta
     4801 aagactaact tgctttggca ggaccaggac tggggtggac gctggcgctg gtatgcctgg
     4861 gagactgatc ttgataagac aatccaatgg ctagaagcaa cagcatccac ccccgaccaa
     4921 attaaaactt tacgatactt atattcctgt cgaacaggga gctgcgacaa attagtattg
     4981 gaattagatc caactgcagt gattaattag aatgaagaat ttcagaatca aaggtttttc
     5041 tcaaacgcaa attgttgttt gatctttttg taaccaaggt gctcataaaa ttcgtgagca
     5101 ttggtgcggg tgatattgct ggcgagtact acctgggtgt aacccaatga tttggcccag
     5161 tcttctattg ctgtaaccaa ttgcctagct acgccctttc cacgatgtgt ttcaacaacg
     5221 gctaacccgc cgatctcaat tgcctcaaca acttctgtga gtaagcgaag gtagccatgg
     5281 caaaaaccta caacttgatc gtcaattttt gctactaaga gtaagtgatc atttcgcagt
     5341 agaatatttt tgattctgcc gtttagggta ttttctgaga caccgtatcc aagggctgaa
     5401 ttaataattg ctactaattg agttgcatcg gatgagctcg ctttgtttat tttgatcatg
     5461 ctcagaaagt tggctagcac tattctaata aattaagatt tccattgagg actattggtt
     5521 gaatcgcact catttctgta aaatagtcca ttctaggtat tgggtagagt agaaaaatct
     5581 ttttacctag cccaaatgca tatcccattt ctaaaaaagt attacctcca atgtagccat
     5641 caatatcttt ttttggataa ttaagaatta atattgaatc tgccatctct acttttttaa
     5701 agtgttctct gatgaagtta tattcaatct ttgcactaat tttgtcctca tctttctcca
     5761 ggagggcttc attaacactg ccatcagcca gttcttcagg cagtaaaata gtaaagccac
     5821 gtttttctaa tctcgcttgt gcttccttca tttgttggtg aaaagacatg ctcccgcaga
     5881 ttgtgattgt catnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtgcttc
     6001 cttcatttgt tggtgaaaag acatgctccc gcagattgtg attgtcatga ttatcttgtg
     6061 tactaacgtg gcattcgcag cgcgactact tcaactagag ccttgactac atcttctaag
     6121 tacagttcaa ttatttgatt agttacataa aaagtattga aatcatcgcc atcagaataa
     6181 tgtgcgcgtt tgaaatcttt aataatttgc tcagcctcag tgaagcgatg taaaccatcc
     6241 acttcgatat atattcgtga tggaagaatg gcgatatcga ctgttttgtg accatcaaag
     6301 tattctaact cggcgtcgat cccactgtct cttaatgctt gccacagttt tgtagcatgt
     6361 tgactggcgt atttatgatt gggtaattga atatttgtca tcagcggaag cggtgggatt
     6421 tcccgatttc atcgggataa ccgggttgtt attacaactc ggttgacttc tcaccccacc
     6481 tttcttgctt gtatccgcta cgtgcggatc ctagcggaag cggtgggatt tcccgatttc
     6541 atcgggataa ccgggttgtt atcacaactc ggttgacttc tcatcccacc tttctcgctc
     6601 gtcactttgt tcctcgcgga agcggtggga ttcgaaccca cgaagccttg cggctctggt
     6661 tttcaagacc agtgcaatta accactatgc gacgcttcca acgacgctcc tcacgatcga
     6721 ggatattgta tcaagaattc aacaaattaa accatcgagc ttgattttga ttagaaattg
     6781 ctaaaacatt ttgaaaagca aaaatttcgt gcgtaagcgg gcgatatggt ggtaatggta
     6841 atagtccatt tggtccaagt ctatacaagg tgtagtttgg taacaaattg ataaaatctt
     6901 tgaagaaaac tcggtggtac acatgcattt cgttaaattc aaattgaata agatctattt
     6961 tttgtttggc aatcagttgt tttgctcctt gtagtaccga aagttcgtaa ccctctacgt
     7021 caattttaag aagatcgata cttgccactt tttgtttttt cagaaaatca tcgagagcga
     7081 tttgttttac atcataggcc ttggattttt gatgatggaa atcttcaata actgctttgc
     7141 ttgtgcttgc aagcgtggcg gtaggttggg ttgttttcaa gaccgcatca tcggcgaagt
     7201 catacaactt aatggttttt tctgtagcac caaccgctaa ttgatgcaat tgcactcggc
     7261 taggatagtt tttcttctga agttgagaaa aagtttctgg gttcggttca aacccaataa
     7321 atcgaacgcg tttgggtaat cgatcataac catagatgct cacatttgcg cccacatcaa
     7381 ccactgtttt tatatcggac ctagcaagac gatctagtaa ccaattttct cccgttacat
     7441 catcgccatc ggcgttgagt agcccaagac cgcgaagcat caacttatga ccatgataaa
     7501 acaacttctg ccagccaggc cgacaaaacc aaagggcaaa ccaccgatta atgttgaaac
     7561 caagagattt tccttgcata gtaaattatt cgtgcaggta gttagcttgt taccactgat
     7621 gtttgatcag tctgccaaat ttcattgaaa aatataatga taaagcatca ataccagctt
     7681 ttaaaattac tttcaggttg gctcccgttg ggttgcctcg caggcgagga tagtgcgtta
     7741 ctggcaattg tttaatttta atgtgatttt ttttcaactt atacagtaat tcagcagata
     7801 tgaaggcgcc atctgattgc aactttagtg gtttaatgag ttcggtttta aacaatttat
     7861 aagcacaatc tatgtcttta acgtgaacac ggaaaagcaa gttgataaat actttgaaga
     7921 gataggcatt tcgcgcgcga ccaaagcctt ctgcgcgcgt cttccggtat ccgatcaccg
     7981 cgctatacaa atcagtaaat tctagaaaac tctccaattc tgagaaatta aattgtaaat
     8041 ccgcatcagt aaagaaaacc cattcgtatt tagcttgttc gaatccagtt tttaatgccg
     8101 ctccataccc aagattagtt gcgtgcgaga taactcgaat atgtgcattc atctctgctt
     8161 gtgactgggc tatggctttt gtattgtctt gactaccatc atctacaaca attaattcgt
     8221 aagtattcgc aaactttgga atgtactcca aagcttgttc caacaaatgc ggtatatttt
     8281 cttcttcgtt gaaggcgggg aaaaacacag ataatgaaga aagttttttc atcactggat
     8341 atcatacctt gcgtggggaa aaatgtcatt gagttaaacc agataaatac gctatactaa
     8401 tggacgtatg agtgaaaagc accaccattc cccatatcaa gtaggtattt tctttgttct
     8461 ctttggtctc attggtgttc tttcgtttat tgtcttacaa ccatacttaa gtctggtttt
     8521 ttttagttta atctactcga ttcttatttg gcccatctac caaagagttt cgaaacgtct
     8581 tggaagtaga aagtcgctat caacgctagc gtctatcgtc ttaagcattt tgctattagt
     8641 gattccaggt attatttttg cacaaacact ggtgattcag gctgtgacag tcgtaacgga
     8701 tttgcaaagc caagttggtc ccgaaactct tgagcaggta gttactcaga tcaattccag
     8761 cgtgaaggta cttccatttg ctcaagattt agaagtgact tcacagcaac tggttcaact
     8821 tattgttcaa aatcttcagc cggctacaga ctggtttctc agctcatttt ggcaaattgg
     8881 taatttttct gtaacattca tgatcaattt agttctactg ataatattag tgtactttat
     8941 tttgccgaat cttcctgact taaaacgata tatatataaa ataagtccat tgagtaaaga
     9001 tgctacccat caatattttt ctcgctccta cgcaatgctt ctcgatgtaa taaaaggaac
     9061 atttttaatc gcaattttgc agggtttggt tggtggtatc tttctagcca ttattggtat
     9121 tccagcatgg gtactgtttg cttttttgat gatggtgctt tccatgattc ccattttggg
     9181 aacaggcttt gtgctagtgc caattggact tgttttatta ttgaccggtc atccttttct
     9241 tggcatttta acaatgtgtt ggcaattttt tgtagtggga acaatcgata acgtcgttcg
     9301 ttcaatggtg gtttcaaaag tggctaacgt tcatcctgcg cttatgatgg tggcggtatt
     9361 gggaggaatc aaggcgtttg gtttcttagg attggtgtat ggtcctctaa ttctcgtggt
     9421 gtttgtaact accttggaag tgtatcgcca agaataccat caatgagcca gtgtttctgt
     9481 gaggaaatct cttaattttt gcgccatgtc ttcacgatgt aagccaatcg aaatattggc
     9541 ttttaaccac tcaaatgggt tacctccatc taagtacgtt ccggttggta catgaatagc
     9601 cacgtcgttt gcttgggcga gttgggtaat agtatccgta atgtacagtt cgccagaatt
     9661 tacatcgagt tgctggttgg cgagtacttc aaacacttgg ggggtgaata tgtatttact
     9721 aatattcact aaatttgatg gttctgagcc aggttgtggt ttttcaacaa tcgattcaag
     9781 taatacatag tcgccatgtt ttttaggttt gatcacacca tagcgcgata attcgctcgt
     9841 aggtttttct acacaggtgg ctacagcaac agcatgagat ttttccatta gttgaatcat
     9901 cgcggcggct tctgagtttc cattggagtt atacattagg tcatcaccca taaacacaaa
     9961 aaatgcgtca tctgtttgta ataacgattg ggcaagtttg agtgggactg cggtgccgta
    10021 ctgttcagaa tcactttgtt taataaaatg aaatgtagct ttttggtgca attttgcaac
    10081 ttggtcatag agcgaggcct tatttcgatc ggctagatag ttatataggc gttgattttc
    10141 ccgatagtag tgaatgagtt gtttgttgtg ctcggaaacc acaaaaatga tatcagtcac
    10201 tccggcagcg agtagatcgt ccaccacata gtcgatgatc ggacgatcta agaatggcag
    10261 catttctttt tgaatagttt ttgtgattgg taagaagcga gtgccaaatc ccgccgcagt
    10321 aattaaggcc ttggtaattt tcatagtcgt aagtatacta tgattaccgc atgacagaaa
    10381 ccaagacagc aagggaaaca attctctcaa ctacaaattt catccattgt ggtgatgatt
    10441 atttgtttat acatcgtaca aaaaaaggta gtagtgttga ttttggtcga ttaaatggta
    10501 ttgggggaaa agttgaacct ggcgaaacgt ttgttgcttg tgccatcagg gaaactctgg
    10561 aagaaactgg ttatgaagtg cctcaggaga attgtcagct cagagcagta gtcaatctga
    10621 gtggcggtta cgctcaagat tggatgatgt gttttttttc aatgactgta gaaacaaaaa
    10681 atgtaccatt aggcatggaa aatccagaag gaaatctttt gtggttacca gctagtcagg
    10741 tacttacttc gggctatgag ttagtcgatg acttacagta catctggacc gacttagtgg
    10801 aagattcacg agtgctattt tttgccgctc agttgaataa acaagaaaag atcacacagc
    10861 attcgatttt tcatctataa tgaggtaact cgccagtcgt ggtcgggttt gttgtggaag
    10921 cttatatgtc tggatggttt aatctagctc ttgttttgtt ggcagttatt tgtctggcat
    10981 tgcttggaat ctggcaactt gggtatgatc caattgctca tttgagttgt gtgaaattgg
    11041 aagattcata tctttcgatt aaaaagaccg gagagaaaac tggtcgatat gtttgcgtac
    11101 atactttctc cgatggaggt aaaacctgtg ttagttccaa tgaatgcgag ggagattgta
    11161 tggtaacaga acaaaccaag atagaatctg atggatttta tgcttggtct caacaattta
    11221 acgcaacgga tagaatatcc ggatgcacaa tacttac
//
LOCUS       DAOQZP010000032        11069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_6343_length_11069_cov_4.752769, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000032 DAOQZP010000000
VERSION     DAOQZP010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 11069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11069
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6343_length_11069_cov_4.752769"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PKH60_03145"
     CDS             complement(<1..328)
                     /locus_tag="PKH60_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNQ17074.1"
                     /translation="MQDNHSTINFSITEKVLGTNHSQVIIEPLEQGYGHTLGNSLRRA
                     LLSSLPGTAFTTVKFSDVDHQFSTLPGVKEDVLEITLNLKSISLNSLTETGGVARLSV
                     VGPKKVT"
     gene            complement(340..945)
                     /gene="rpsD"
                     /locus_tag="PKH60_03150"
     CDS             complement(340..945)
                     /gene="rpsD"
                     /locus_tag="PKH60_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNQ17075.1"
                     /translation="MARYIGPKNKLARKIGEDLGLRTNPLKVGKRLNVRPGQHGAKSR
                     RKQSDYAIQLKEKQKVKYIYGILEQQLRKLYTEASQRKTATGATLLSYLERRLDNVLY
                     RIGWAPTRAAARQIVNHGHVRVNSKKMSIPSYRVQVNDVVSLTDSALHIPTVEALLKD
                     GVSRVEWLERKAHIGKVVRAPLRNDIQEKIEEQLVIEYYSR"
     gene            complement(958..1359)
                     /gene="rpsK"
                     /locus_tag="PKH60_03155"
     CDS             complement(958..1359)
                     /gene="rpsK"
                     /locus_tag="PKH60_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014953338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNQ17076.1"
                     /translation="MANTNKPAAKNKKKATRTVPEGKLYVTATFNSTLVSVTDEKGNV
                     LCWSSTGESGFSGSRKSTPYAATITMENAINKARQFGMNTMAVFIKGPGPGRDVALRV
                     LRAQRIRVTQIVDQTPMPHNGTRPRKAKHNK"
     gene            complement(1379..1783)
                     /gene="rpsM"
                     /locus_tag="PKH60_03160"
     CDS             complement(1379..1783)
                     /gene="rpsM"
                     /locus_tag="PKH60_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNQ17077.1"
                     /translation="MSRILGVDLPEQKKIGFALLSIFGVGRNRSTQALKEANVDPEKR
                     TRDLTTLELSRIQRVLERFRLEGDLRREESDNIDRLIRIQCYRGKRHQAKLPSRGQRT
                     RTNGRTARGGGKRRTVGSLTKEQATKIETKEK"
     gene            complement(1790..1906)
                     /gene="rpmJ"
                     /locus_tag="PKH60_03165"
     CDS             complement(1790..1906)
                     /gene="rpmJ"
                     /locus_tag="PKH60_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNQ17078.1"
                     /translation="MKVRSSVKKLCIQCKIIRRKGAVRVICKGNPKHKQRQG"
     gene            complement(1970..2191)
                     /gene="infA"
                     /locus_tag="PKH60_03170"
     CDS             complement(1970..2191)
                     /gene="infA"
                     /locus_tag="PKH60_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939491.1"
                     /note="stimulates the activities of the other two
                     initiation factors, IF-2 and IF-3; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNQ17079.1"
                     /translation="MVEACLPNTMFRVKVTDSVAKPMIGRMLLGTLVGKMRLYRIRVM
                     PGDLVRGYVSKYDLEKCKITYRSTKTDSQ"
     gene            complement(2331..3095)
                     /gene="map"
                     /locus_tag="PKH60_03175"
     CDS             complement(2331..3095)
                     /gene="map"
                     /locus_tag="PKH60_03175"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNQ17080.1"
                     /translation="MSTRESATSSKSRISAMEVGGAALAQIKSKLREFAQVGVSFIDI
                     EKQATALIQEAGMQPSFSTVPGYHWSTCIMKNDALCHGIPDNQIIANGDIITIDVGLI
                     NQGYHLDTTISFGVGEITTKQRRFLEIGQKSLSKAIACAKVGQSVYQISRAMEKVLLE
                     ENYGAVYQLTGHGVGKELHMEPTIPCVANPEDRKVRLAEGQTLAIEVMYAMGNPQLVV
                     DADEWTFRTKDGSLTAMFEETVLVTAHGPEILTKGR"
     gene            complement(3107..3652)
                     /locus_tag="PKH60_03180"
     CDS             complement(3107..3652)
                     /locus_tag="PKH60_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HNQ17081.1"
                     /translation="MRIILIGIQGAGKSTQGNLLATEYNVPYLSSGHIFREMAKEKTQ
                     LGRWLKETLNSGALVPDDTTLEIVLTYLEKPEYINGYILDGFPRTVPQAKAFNGAVDF
                     VVFLAVSDKEALWRISGRNDVREDETLQALRKRIQLFHELTAPVVEYYRGEGKLIEVD
                     GEVEVDEVFSQIKTAIVNKSK"
     gene            complement(3670..4923)
                     /gene="secY"
                     /locus_tag="PKH60_03185"
     CDS             complement(3670..4923)
                     /gene="secY"
                     /locus_tag="PKH60_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNQ17082.1"
                     /translation="MWHTKALRNKLLFTATMFLIFRFLAHVPLPGVDLLKLGSIFENN
                     QFLNLLNIFSGGTLSNFSIVAVGINPYITASIIIQLATMVFPKLKEIQKDGESGRERI
                     NQYTRLASVPLAIIQSVSVLTLLRSQELIQIDNPLLLVGLIATLVAGSMIVLWLGELV
                     SLYGIGNGISMILLGGILSRLPVTVAELFTTGAGDQSFTAIIVLALFIAIIAMVVFMN
                     ESVRKVTIQYAKRQRGSRVYGDYMTHLPIRVNVSGVMPIIFALSIMIAPSFIGRLLVA
                     SQQVQLVILGQKLQLWFAQTNPVYIVSYFLVVFFFSFFSALIFFNAEDLSSELKKSGA
                     FLPGVRPGAATKKYLEFVVNRITLAGAFFLGFIAILPSLVQNATGIQSLALGGTSALI
                     VVSVILETAKQVESMLVEQNYDQYR"
     gene            complement(4962..5402)
                     /gene="rplO"
                     /locus_tag="PKH60_03190"
     CDS             complement(4962..5402)
                     /gene="rplO"
                     /locus_tag="PKH60_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNQ17083.1"
                     /translation="MSRLQSLLAITKRTNKRVGRGYGSGKGGHTSTRGMKGQNSRNGG
                     KRPLWFEGGQLPLIKRLPMWRGKGKLESFERTAAVSLTELSKMQATNISLDTLKLEKV
                     IAQRFRGAKVVAHGSVKRPVVVTGLKVTQLARRLIEKAGGSVVE"
     gene            complement(5529..6023)
                     /gene="rpsE"
                     /locus_tag="PKH60_03195"
                     /pseudo
     CDS             complement(5529..6023)
                     /gene="rpsE"
                     /locus_tag="PKH60_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            complement(6032..6412)
                     /gene="rplR"
                     /locus_tag="PKH60_03200"
     CDS             complement(6032..6412)
                     /gene="rplR"
                     /locus_tag="PKH60_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010095747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNQ17084.1"
                     /translation="MKNTNYHANDAKARRTKRVRAKLFGTAQRPRVTVYRSNRYISAQ
                     AIDDNAQKTLVFAKDAQQAEKSNKKSETKLTKTQRATVVGEMIAQQLKTMKIQSVVFD
                     RGPYRYHGRIKAVADALRAQGVQV"
     gene            complement(6431..6970)
                     /gene="rplF"
                     /locus_tag="PKH60_03205"
     CDS             complement(6431..6970)
                     /gene="rplF"
                     /locus_tag="PKH60_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNQ17085.1"
                     /translation="MSRIGKKNISIPSGVTIDISPREVVVRGPKGEQRVTVLEGISLA
                     NQDGTLVVSRKNDTRQIRAYHGLVRNLVANAIIGVTDGYKKTMKMVGTGYRVQAKGAG
                     ISLAVGYSHTVEVPAITGITFQVEGNDTIHVSGIDKQQVGQVAANLRAVRPPEPYKGK
                     GIRYENEFVKTKPGKTASA"
     gene            complement(6973..7362)
                     /gene="rpsH"
                     /locus_tag="PKH60_03210"
     CDS             complement(6973..7362)
                     /gene="rpsH"
                     /locus_tag="PKH60_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNQ17086.1"
                     /translation="MTDPIADLLIRIKNAHLAKKSQVSIPDSKVKRAILNILLAEGYI
                     ESFVVAGEVPKQSLEVQLKYVKKQSAVSGVRRLSKPGRRLYVTAKEIPQTLAGYGLTI
                     VSTNKGILSDSQARKQNLGGELICKIW"
     gene            complement(7372..7602)
                     /locus_tag="PKH60_03215"
     CDS             complement(7372..7602)
                     /locus_tag="PKH60_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNQ17087.1"
                     /translation="MAKKSKIAKAKVLRAKRSALLLSGQTKVNRVSTRGENRCKITGR
                     PRGYMGYFGLSRLTFRELALKGELPGVVKASK"
     gene            complement(7595..8158)
                     /gene="rplE"
                     /locus_tag="PKH60_03220"
     CDS             complement(7595..8158)
                     /gene="rplE"
                     /locus_tag="PKH60_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNQ17088.1"
                     /translation="MKITKNDFTTTIVPQLMKELGIKNVFAVPKIKKITLNMGITQPQ
                     DPRARKPIIENVQAQFALISGQKPQISKATKSISGFKLRQGDPVGVMVTLRGDLMWTF
                     LQKLLIVALPRVKDFRGVASTAFDGRGNFSMGIDEQIIFPEINYDTIDRIRSMQVVIV
                     TSTNSDEEARVLLRLLGMPFAKEEKNG"
     gene            complement(8166..8546)
                     /gene="rplX"
                     /locus_tag="PKH60_03225"
     CDS             complement(8166..8546)
                     /gene="rplX"
                     /locus_tag="PKH60_03225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNQ17089.1"
                     /translation="MKIQVNDIVKILTGKDKGKQAKVMSVLPRRQMVVVEGINLVTRH
                     RKPQNGQSGERIILTKPMHVSKVGILNAAGEVDRVGYKILKDGSKIRIYKKSGEEIVS
                     TVKPEVKKTSKPNEKVTKTNKAKK"
     gene            complement(8543..8917)
                     /gene="rplN"
                     /locus_tag="PKH60_03230"
     CDS             complement(8543..8917)
                     /gene="rplN"
                     /locus_tag="PKH60_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNQ17090.1"
                     /translation="MIQLRSVLLVADNSGAKKVRTIQVYGGSKRNTGSVGDIVIASVI
                     QADTQGAIKRGDKVRAVVVRTRKEVRRPNGEYIRFDDNAVVIVESRTSPNPVGTRVFG
                     PIAREVKNRGFNKIASLAPEVL"
     gene            complement(8914..9159)
                     /locus_tag="PKH60_03235"
     CDS             complement(8914..9159)
                     /locus_tag="PKH60_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uS17 family ribosomal protein"
                     /protein_id="HNQ17091.1"
                     /translation="MKQMIGKVSSLPGVQTARVDVSRQWTHPLYKKSVKRTKKYACHF
                     EKLSLVVGDSVVIQPCKPMSKTKHFVIIKKSEMEDKS"
     gene            complement(9156..9356)
                     /locus_tag="PKH60_03240"
     CDS             complement(9156..9356)
                     /locus_tag="PKH60_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNQ17092.1"
                     /translation="MKKQDLPALHEMSLVELRELLEKSQRELTIEKIKNQAGKSKGGS
                     LARASDSLARILTIIREKELLV"
     gene            complement(9361..9765)
                     /gene="rplP"
                     /locus_tag="PKH60_03245"
     CDS             complement(9361..9765)
                     /gene="rplP"
                     /locus_tag="PKH60_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNQ17093.1"
                     /translation="MQQPKKSKYRKAFRGRRKGISLRGNTVVFGEYGLQARSTNWVSA
                     RQIEAARKKITYMTKRAGKYWIRIFADKPTTKKPVGVKMGSGKGAIEQYVAVVRPGMV
                     LFEIGGVPESLAREALRKASHKLSVDTGVIAK"
     gene            complement(9786..10442)
                     /gene="rpsC"
                     /locus_tag="PKH60_03250"
     CDS             complement(9786..10442)
                     /gene="rpsC"
                     /locus_tag="PKH60_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNQ17094.1"
                     /translation="MGQKVNPTGFRLGSTFTWKSRWHSEPAQYSELALEDYELRRYLE
                     AKLANAQVVQVDIERSLTVIKVILFVARPGVVIGKGGANLEEVKKGVEAIVNAPSKNQ
                     KGKVKADIRVEEVTSPELSAKLIAETIVQQLIKRFPHRRAVSQALEKIMQAGAKGVKI
                     SLSGRIAGAEIGRTEKYAQGSVPTQTLRANIDYYQLPAKTRSGYVGIKVWIYKGEVQS
                     "
     gene            complement(10525..11040)
                     /gene="rplV"
                     /locus_tag="PKH60_03255"
     CDS             complement(10525..11040)
                     /gene="rplV"
                     /locus_tag="PKH60_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNQ17095.1"
                     /translation="MQITVVQKSVRQSPRKVRLVVNAVRKLSLEQAILQLGQIQRRST
                     LVVLKTLRQAVANAQHNHGLSASDLKIVSIQVGEGARFKRFQPVSRGRAHSIVKRSCQ
                     ITVILETTESAKPEEKKAKIVEPTKKAAESVKTEISEKVKRPSQPKVEKVTRNAVVVS
                     KQTRTKRPVAK"
BASE COUNT         2885 a         2523 c         1955 g         3706 t
ORIGIN      
        1 ctgttacttt tttcggacca acaactgaaa gtcttgctac gcccccggtt tcggttaacg
       61 aattaagact aattgatttc agattcaaag taatttccaa cacatcttct ttaacaccag
      121 gaagtgttga aaattgatga tcgacatcgg aaaatttaac tgtcgtgaat gcagtaccag
      181 gtaatgaact aagtaatgcg cgacgtaaag aattgcccaa cgtgtgacca tagccctgtt
      241 ctaatggctc aatgattacc tgagagtggt tagttcctaa aaccttttca gtaatagaaa
      301 aattaattgt tgagtgatta tcttgcatac atgctccagt taacgactat aatattcgat
      361 tactaattgt tcttcaattt tttcttgtat atcattgcga agcggggctc tgactacttt
      421 gccaatgtga gcctttcttt caagccactc aactctcgaa acaccatctt tcaatagagc
      481 ttcgactgtt ggaatgtgaa gcgccgaatc agttaatgaa acgacgtcgt ttacttgtac
      541 acgataggag ggtatgctca ttttttttga attaactcgc acgtggccat ggttaactat
      601 ctgtcttgca gctgctcgag ttggcgccca accaattcga tataaaacat tatctagtct
      661 tctttccaaa taagagagta gagtagcgcc tgttgcggtt tttctttgac tggcctctgt
      721 atacaacttt cgaagttgtt gctctaatat tccatagata tatttaactt tttgtttttc
      781 tttgagttga atcgcataat ctgattgttt tcgacgtgat tttgcgccgt gctgaccagg
      841 tcgaacgttt aaccttttcc ccactttcaa aggattggta cgaagaccca gatcttcacc
      901 gatttttctt gctagcttgt tttttggtcc aatatatctt gccataagat tactttatta
      961 tttattgtgt tttgccttac gtggccgagt accattgtga ggcatgggcg tctgatctac
     1021 tatctgagta actctaattc gttgtgctcg tagcaccctg agggccacat ccctaccagg
     1081 tcccggtcct ttaataaaca cagccatcgt attcattcca aattgtcttg ccttattgat
     1141 tgcattttcc atggtaatgg tagcggcata gggtgtagat tttcttgacc cactaaatcc
     1201 agactcacca gttgaagacc aacataaaac attgcctttt tcatcagtga cactaaccag
     1261 tgtactatta aaagtagccg taacgtatag tttgccttca ggcacagttc tcgtagcctt
     1321 ctttttattt tttgcagctg gtttatttgt atttgccata cgcttaaaat tccgttgatt
     1381 acttttcttt tgtttctatc ttagttgctt gttccttagt aagtgaacct acggttcttc
     1441 tcttaccacc accacgagca gttcgaccat ttgttcttgt ccgctgacct ctacttggaa
     1501 gtttagcttg atgccgtttg cctcgataac actgaattct gatgaggcgg tcaatattat
     1561 cactttcttc tcttctaaga tcaccttcta acctaaatct ttccaagact cgctgaattc
     1621 tgctcaattc taacgtagta agatctcggg ttcttttttc tggatcgaca ttggcttcct
     1681 tgagtgcttg ggtggatcga ttgcgaccca ctccaaaaat actaagaaga gcaaagccaa
     1741 tctttttctg ttctggtaaa tctactccta atatgcgtga catgtatatt tatccttgtc
     1801 tctgtttgtg ctttgggtta cccttacaaa taactcgcac cgctcccttg cggcgaataa
     1861 ttttacattg aatacagagt ttttttactg acgatcttac tttcatatgt ttgactcaaa
     1921 aacaggcctg aactatacca gtcttggcgt aaattgaaaa gacccaattt tactgcgaat
     1981 ctgtttttgt tgatctatag gttattttac acttttcgag gtcatatttt gatacatatc
     2041 cgcgaactaa gtcacctggc ataactcgaa ttcggtacaa tctcatctta ccaacaagcg
     2101 tacccaagag catgcgccct atcataggtt tagccacact gtcagttact ttgactcgaa
     2161 acatagtgtt aggcaaacag gcttcaacca caccctcaac ctcaaatcgg tctggtcgat
     2221 cggaacgctc agttgttgtg tcaacttgct tccgagcttt ggctgatcgt ttatgttctt
     2281 gaactttacc catagattat ttcattgttt aaggggagta gtatatcaaa ttatcggcct
     2341 tttgtcagta tctccggtcc atgcgcggtt accaataccg tctcctcaaa cattgctgtt
     2401 aacgaaccat cttttgttct gaaggtccat tcatctgcat caactaccaa ttgaggattt
     2461 cccatggcat acataacctc aattgcaagg gtttgaccct cggccaacct cactttgcga
     2521 tcctctggat tcgcaacgca gggaatggta ggttccatgt ggagctcttt tcccacccca
     2581 tgtccagtaa gctgataaac agctccatag ttttcttcta acaacacttt ttccattgcg
     2641 cgactaattt gatacaccga ttgaccaact tttgcgcatg caattgcctt actcaatgat
     2701 ttttgtccaa tttccaaaaa tctacgttgt ttagtggtaa tttctccgac cccaaaactt
     2761 atggtggtgt ctagatgata accttggttt atcaaaccaa catcaattgt aattatgtct
     2821 ccattagcaa tgatttggtt atcgggtata ccatgacaca atgcgtcatt tttcatgata
     2881 caggttgacc agtggtatcc ggggactgta gaaaaacttg gttgcatacc agcctcttgt
     2941 atcaaagcag tagcttgttt ttcaatgtca ataaaagaaa cacccacttg agcaaactct
     3001 cgtaatttag acttaatctg cgccagagca gcaccaccaa cttccatagc cgatattcgg
     3061 cttttagatg atgtcgcaga ttctcgagtt gacacaaaat gcttaattat ttacttttgt
     3121 ttacaatagc ggttttgatt tgggaaaaca cttcatcaac ttccacttca ccgtcaacct
     3181 caattagttt tccttctcct cgatagtatt cgactaccgg cgctgttaat tcatgaaata
     3241 attgaattcg ttttcgaaga gcttggagcg tctcatcttc acgcacatca ttgcgaccag
     3301 aaatcctcca aagtgcttct ttatcagaaa ctgccaaaaa tactacaaaa tccacggcgc
     3361 cattaaaggc cttcgcttgt ggtacagtgc gaggaaaacc atccaaaatg taaccattga
     3421 tatattcggg cttctccaaa tatgttaaaa ctatttctag tgttgtatcg tctggaacca
     3481 atgcgccaga gttaagcgtt tcttttaacc atcgaccaag ttgcgtcttc tcttttgcca
     3541 tttcgcgaaa tatatgacct gaggaaagat acggcacatt atattctgtg gccagcagat
     3601 ttccctgagt tgatttacct gctccctgaa ttccaatgag gataattctc atagttctct
     3661 ccttgtcttt taccggtatt ggtcgtaatt ttgctcaacg agcatacttt ctacttgttt
     3721 agctgtttct agtatgactg atactacgat caaagcgctg gtaccgccta aggcgagtga
     3781 ctgaatacct gtcgcatttt ggactaagga tggcaaaatc gctataaatc ctaaaaagaa
     3841 agctcctgcc agtgtaatcc gattaactac aaattctaaa tatttttttg tagctgctcc
     3901 gggtctaact cctggtagaa aagctccact ttttttcaat tctgacgaca aatcttcagc
     3961 attaaaaaag atgagagcgg aaaaaaatga gaaaaagaaa acaactaaga aataactcac
     4021 tatatacact gggttggttt gcgcaaacca gagttgtaat ttttgaccaa gtattacgag
     4081 ctgtacttgt tgtgaagcaa ctaacaatct gccaataaat gatggagcta tcataattga
     4141 cagagcaaaa ataataggca tcacaccaga tacattgact ctaatcggta agtgggtcat
     4201 gtaatcgcca tacaccctgc tacctctttg acgcttcgcg tattgaattg ttacttttcg
     4261 caccgattcg ttcataaata caaccatagc aataattgca ataaataagg ccaaaacaat
     4321 tatggcagtg aagctctgat cgcctgcgcc agtggtaaac aactccgcga ctgtcacagg
     4381 caagcgactg agaatacccc ccaacaggat cattgatata ccattgccaa taccatagag
     4441 ggaaactagt tctcccaacc agaggacaat cattgagcct gcgacgagtg tggcaattaa
     4501 ccccacaagc aacagagggt tatctatttg aatgagctct tgtgaacgca gtagagtaag
     4561 taccgaaaca ctctgaatga tggctaaagg tactgaagca agacgagtgt attgattgat
     4621 tctttctctc ccagactctc catctttctg aatttccttc agcttgggaa aaaccatagt
     4681 ggctaattgg ataataatag aagcagtaat gtaagggtta attccaacag ctacaatcga
     4741 aaaattagac agggtgccac cagaaaatat gtttagtaaa tttaaaaatt ggttattttc
     4801 aaaaatagaa cccaatttta ataagtctac tccaggaagt ggtacatgag ctaagaaacg
     4861 aaatatcaaa aacatagtag cagtgaacaa tagcttattt cgtaaagcct tggtgtgcca
     4921 cattttttgc aggagattag ttagcctttg cattaaggtg tttactctac cacagaacca
     4981 cccgcttttt caatcaaacg gcgtgccagt tgagtgacct tcaaaccagt aacaactact
     5041 ggtcttttta cagaaccatg agctaccacc tttgctccac gaaatcgttg agcaattact
     5101 ttttcaagct tcagtgtatc cagagaaata ttggtagctt gcatctttga aagctcagtg
     5161 aggctcactg cagcagttct ctcaaatgac tctagctttc ctttacctcg ccacattggc
     5221 aaacgcttga tcagtggcaa ttgaccacct tcaaaccaca acggtcgctt gcctccatta
     5281 cgagagttct gacccttcat accccgagtg ctggtgtgac cacctttgcc ggatccgtaa
     5341 cctcgaccaa cccgcttatt ggttcgtttg gtgattgcta gtaatgattg taaccttgac
     5401 atagtgcttt tcaattattt ttttgtaggc gtttttcggg caattcgctt ttcttcaacc
     5461 tttgcctcag tttttcgctc agcgttttgt tccatttctg ccttaaattt ctgttctgtt
     5521 tcggcttcaa cctgggcaat tgattttaat gtaattactt tcaaatcaac aagtcgtgca
     5581 atttccttaa gcgcagcaaa agttgcatga acgcttgaag ccttattttg tgatccatga
     5641 attttactag aaatatctct aatacctgca gcctcgacaa ctgatctcac aggtccacca
     5701 gcaataacac ctgaccctct tggagcaggt ttaagcaaaa cttttccagc tcctttttta
     5761 atatcaaccg aaaaagggat agtggtgccg tctaagggca cagaaatcat cttctttttc
     5821 gcttttttta ctcccttgcg aattgcagca gcaacatcag gtgccttccc caaaccgacc
     5881 cccacttgac ccttccgatc acccacaacc ataagagctg aaaaaccgat ttggtttcca
     5941 cccttagttt tttttgatac tcgagagatc tgaattactt tctcttcgaa ttcgtttgaa
     6001 tctgatgatt gtcttggtct catacttggt ctcatacttg tactccttgt gcgcgaagtg
     6061 catcggccac tgctttgatc cgaccatgat aacgatatgg tcctcgatca aaaaccactg
     6121 attgaatttt cattgttttg agttgttgag caatcatttc tcccaccacg gtagctcttt
     6181 gagtttttgt cagtttggtc tcagattttt tatttgattt ctctgcttgc tgagcgtcct
     6241 tagcaaaaac caatgttttt tgcgcgttgt catcgattgc ctgagccgaa atgtagcggt
     6301 tagaacgata gacagttact ctaggtcttt gagcagtacc aaaaagcttc gcccgaaccc
     6361 tcttcgttct tcgtgctttg gcatcattgg cgtgataatt tgtatttttc atgtttgtgc
     6421 tctcaagcga ttatgccgaa gcagttttgc ctggcttagt ttttacgaat tcattttcgt
     6481 atcgaattcc cttacccttg tagggttcgg gtggacgcac tgcccgaagg ttggcagcta
     6541 cttgcccaac ttgttgctta tctattccag acacatgaat ggtgtcatta ccttcaacct
     6601 gaaaagtaat ccccgtaatt gctggcactt caacagtatg tgaatatccc acagccaaac
     6661 tgatgccagc gccttttgcc tgaactctat atccagttcc aaccattttc atagtttttt
     6721 tataaccatc agtaacacca atgattgcgt tagcaactaa gtttctcacc aatccgtgat
     6781 aagctctgat ttgtcgtgta tcattttttc gacttaccac cagcgtccca tcttgattag
     6841 ctagtgagat accttctagc accgtaactc tttgttctcc ctttggacct ctcacaacga
     6901 cctctcttgg agaaatgtcg atagtgacgc cactaggaat gctgatattt tttttaccta
     6961 ttcttgacat aattaccaga ttttacaaat tagttctcca cctaaatttt gttttcttgc
     7021 ttgtgaatca gaaaggattc ctttattagt agaaacaatc gttaatccat aacctgccaa
     7081 ggtttgtggt atttctttgg cggtcacata taatcttcga cccggttttg aaagtctgcg
     7141 cacaccactg accgcagatt gtttcttgac atattttaat tgcacctcaa gagattgttt
     7201 gggcacttct cccgccacca caaatgattc aatataccct tcggctaaca atatatttaa
     7261 aattgctcgc ttgacctttg aatcaggaat actaacctgc gatttctttg ccagatgagc
     7321 gttttttatt cgaattagta ggtctgcgat tggatcagtc ataactttaa cttatttact
     7381 tgctttaaca actcctggca actcaccttt tagagctagt tcccgaaatg ttagtctact
     7441 taaaccaaaa tagcccatat acccacgggg tcttccagta attttacagc gattttctcc
     7501 tctggtagaa actctattaa ctttcgtttg accagacagt aacaaagcgc tacgttttgc
     7561 ccgtaatact tttgcttttg ctatttttga ttttttagcc atttttctcc tccttggcaa
     7621 atggcatacc caataaacgc agtaaaactc tcgcttcttc gtcggagttt gtactggtta
     7681 ctatcaccac ttgcattgaa cgaattcggt cgattgtatc ataatttatc tcagggaaaa
     7741 tgatctgttc atcaatgccc atactaaaat tacccctgcc atcaaatgca gtcgaggcaa
     7801 ctcccctaaa atctttcacc cgtggcaaag ccactattaa taatttctgt aaaaaagtcc
     7861 acattaaatc acctcgcaat gtgaccatca cacctacggg atcaccttga cgtagtttga
     7921 aaccagaaat tgatttagtc gctttactaa tttgaggttt ttgacctgaa attagtgcaa
     7981 attgggcttg aacgttttca ataatcggtt ttctagctct gggatcttga ggttgggtaa
     8041 tacccatatt cagggtgatt tttttgattt taggcactgc aaagacattc tttataccca
     8101 gctccttcat tagctgaggc acaattgtgg tggtaaaatc gtttttagtt atcttcatat
     8161 actcgttact ttttagcttt gttagttttt gttactttct cattaggttt tgaggttttt
     8221 tttacctcag gtttcaccgt acttacaatt tcctcaccac tttttttata gattcttatt
     8281 ttagaaccat ctttcaagat tttataacca actctatcaa cttctcccgc agcattcagt
     8341 attccaactt tactcacatg cattggtttc gttaaaataa tgcgctcacc agattggcca
     8401 ttttgcggtt ttctatgcct ggtcactaaa tttatacctt caacaacaac catctgtctt
     8461 cttggtaaaa ccgacataac ttttgcctgt ttacccttgt ctttgccggt taaaatttta
     8521 acgatgtcgt tgacttgaat tttcataata cctctggtgc caagctagca attttattaa
     8581 atcctctgtt cttcacttct ctagcaatcg gaccaaaaac acgggttcca acggggttag
     8641 gacttgttct tgattcaacg attacaacag cattgtcatc aaaacgtatg tattcaccgt
     8701 ttggacgtcg cacttctttt ctcgttctga ccaccacggc tcttacttta tctccacgct
     8761 taatagctcc ctgtgtgtcg gcctgaataa ctgatgcaat tacaatatca cccacagaac
     8821 ccgtatttct ttttgagcca ccatagacct ggatggttct aactttttta gcaccagaat
     8881 tatctgcaac tagtagtact gaccgaagct gaatcatgat ttatcttcca tttctgattt
     8941 ttttatgata acaaagtgtt tcgtcttact catcggctta cacggttgaa tcaccactga
     9001 gtcaccaaca actaaagata gtttttcaaa atggcacgca tatttctttg ttcgttttac
     9061 agatttttta tatagtggat gcgtccactg acggctaacg tcaactcgcg cagtttgaac
     9121 cccaggtaaa gagctgactt tgcctatcat ttgtttcata ctaataattc cttttctcta
     9181 atgatggtga gtatgcgagc cagactatct gatgctctgg ccaaacttcc acccttactc
     9241 tttccggctt gatttttaat tttttcaatg gttaactctc gctgcgactt ttctaataat
     9301 tctcgcagtt caaccaatga catttcgtgg agtgctggta aatcttgttt tttcatataa
     9361 ttactttgca atcactccag tatcgactga taatttatgt gatgctttac gtagagcttc
     9421 gcgagctagt gactcaggca ctccaccaat ttcaaacaac accattccag gtctcaccac
     9481 cgcaacatat tgttcgatcg caccttttcc tgatcccatt ttaacgccaa ctggcttctt
     9541 cgtagtcggt ttatcagcaa agattctgat ccagtactta cccgctcttt ttgtcatata
     9601 cgtaatcttt tttcgagccg cttcaatctg cctagctgat acccaattgg ttgagcgagc
     9661 ttgtaaacca tattcaccaa acaccactgt gttaccccgt aaagaaattc ctttgcggcg
     9721 tcctctaaat gctttgcggt atttgctttt ctttggttgt tgcatacagt tatttgtttc
     9781 tcaagctatg actgaacctc tcccttatat atccacactt tgatgccaac atatccagat
     9841 ctggtttttg ctggtaattg atagtaatca atgtttgctc tcagcgtctg agttggaaca
     9901 cttccctggg catatttctc agttcgacca atttcagctc cagcaattct tcctgagagt
     9961 gaaatcttca cgccttttgc cccggcttgc attatttttt ctaatgcctg actaacggct
    10021 cgacgatgag gaaagcgttt aatgagctgt tgcacgattg tctcagcaat taattttgcg
    10081 cttaactcag gactggttac ttcttctact ctgatatctg ccttgacttt acctttttga
    10141 ttttttgaag gtgcattaac aattgcctca actccttttt tcacttcttc aagatttgcg
    10201 ccacctttac caattaccac acctggtctc gcgacaaaca aaattacttt gataactgtc
    10261 aaagatctct cgatgtcaac ttgaacaacc tgagcatttg ccagtttagc ttctaaatag
    10321 cgacgcaatt cataatcctc gagtgctaac tcagaatatt gagctggttc gctatgccaa
    10381 cgagacttcc acgtaaatgt ggaacctaaa cgaaagccgg ttggatttac tttttgtccc
    10441 atgtatttcc ttagttgtta ttgtgtctta aataaagagt ggttattgta cagtacaagc
    10501 caactttatt ccagatacag agctctattt agctacaggt ctttttgtcc tagtttgttt
    10561 actaactact actgcgttac gagttacttt ttcaactttt ggttgagatg gtcgtttgac
    10621 cttctcgcta atttcagttt tcactgattc tgctgccttt ttagttggtt caactatttt
    10681 cgcttttttc tcttctggtt tagccgactc ggtagtttct aaaatcaccg taatttggca
    10741 agatcgtttt acgatgctat gagctcttcc tcgactaacg ggttgaaagc gtttaaatcg
    10801 agcgccttca ccaacctgaa tagatacaat ttttaaatcc gatgcactca ggccgtgatt
    10861 gtgttgcgcg tttgccactg cttgtctaag agtttttaat accaccaaag ttgatcttcg
    10921 ttgaatttga cccaattgca aaatagcctg ttccaatgat agctttctga cagcatttac
    10981 caccaagcga acttttcttg gtgattgacg aactgatttt tggacaactg taatctgcat
    11041 ataatctttt tatgtctttg agagtacgc
//
LOCUS       DAOQZP010000033        10589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_6726_length_10589_cov_6.345927, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000033 DAOQZP010000000
VERSION     DAOQZP010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 10589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10589
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6726_length_10589_cov_6.345927"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PKH60_03260"
     CDS             complement(<1..341)
                     /locus_tag="PKH60_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17096.1"
                     /translation="MKSRIFKLKTQLLSTLKKRWKLILVLLLVLGGGAFFYFQKQQAA
                     KPEITTTNPERKTLVKTLDVSGIVDAKEKANLRFLAGGKITYLGAKEGSPVKKWQTIA
                     TIDQATLQKQF"
     gene            410..483
                     /locus_tag="PKH60_03265"
     tRNA            410..483
                     /locus_tag="PKH60_03265"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:444..446,aa:Ile,seq:tat)
     gene            850..1440
                     /locus_tag="PKH60_03270"
     CDS             850..1440
                     /locus_tag="PKH60_03270"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNQ17097.1"
                     /translation="MSTYYLVRHGLRVSRDEDTLLSDMGIRQAELTGEYLQSKNIHHI
                     FVSPVKRTLHTAEIIYSYLQVPQSTDARLKERMEYEPKQGSFESFLGEWDKTMADRMY
                     QPPYGDSAVHAGDRIVSLLEEINDDNNYVCISHGGVIGDVVRNLFPERLYALKADPIK
                     PLKWLNIPEASVTEIQKIGDTYTLKMVGNISHLTRK"
     gene            complement(1449..2012)
                     /locus_tag="PKH60_03275"
     CDS             complement(1449..2012)
                     /locus_tag="PKH60_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HNQ17098.1"
                     /translation="MKVLKALLPKVGDQLLMVLEKRFNQNPDRHPDLKWEKIKAKLKI
                     NPTKLWSLNEMERTGGEPDVVGQDNTTGEYLFIDCAVESPSGRRSCCYDKEALDSRKE
                     HKPELSALGMSTDMGIELLTETQYKKLQQLGEFDTKTSSWIKTPAEIRKLGGALFADR
                     RYNTVFVYHNGASSYYSSRGFRGWLAI"
     gene            complement(2027..2710)
                     /locus_tag="PKH60_03280"
     CDS             complement(2027..2710)
                     /locus_tag="PKH60_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17099.1"
                     /translation="MSRSFFLIGIFLIVVAGGLLFLQQNDNPEGNQSDQTTNLALSEP
                     KITQVAMLADCTVQLTINGNMKTLPTAFDPAIAKCEKYTVSSLSPSGNYVAFEDLSSS
                     GIDAVVSLYELSNDRVYTLDDFGTESVLDMVFLPSNRLLVLYSQGISGKQSLRLYDVP
                     SLEPLLVDVPPEGDLSTETLNPFVFERDLDDAKGIAKAIRLTDTSVVLVDEFDDITNP
                     LFEISLSEL"
     gene            complement(2738..4546)
                     /locus_tag="PKH60_03285"
     CDS             complement(2738..4546)
                     /locus_tag="PKH60_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17100.1"
                     /translation="MVNATTQTTNSTQLPPTLQPVSAGPQPPSPTQAAVMQTTKPNGI
                     NQMKKNFKLAMAGLMMFVLLAGGGVALFLSQEEQDTRNQASTDACMDAPLARNPAVTK
                     VNDTQFKLQWNQVAGASGYRIFLITEGEAGTHPSGKYIIFNVLDNSSEVSETPQFAPI
                     VPANAKSLSPTQTEYVWTLPTNDVIDLEGKVVRERPYYRFLVFAFKTGTSCTSQAAIG
                     TYTSPAATPTPTSTFVGACTENRPVVDDRQVNWIENNSKIQLSWKLTSGEVTDYEIWN
                     ISYEPLNEQAPEKYYLSNLTTQPLPRTATSYTVANPNTLKTRRFLIRAVSQRNAQRAP
                     ACYDQWALCLPPVVLDRKVTMPDKNTALFEWNPVQAPQQKYQIWLIAEGTPEEIGNDR
                     YYIALLADNVDPNSKSLRLSLPEKYQNLPSYRFLIRAINYSADGAPQCYNQWALAPGA
                     IGGPPVGQCTLQFTITGPTATPNPSATATPTTNPSSTPTPTPTTVPGATATPTTRVTP
                     VIGCNAICVTNADCSNPAHICFVVNETENRCRLESNPMDMNCQNPVAAVTEPAEVAQQ
                     PALPETLPETGPEDWGNWLKAGLAILGIGAALLLML"
     gene            complement(4550..5911)
                     /locus_tag="PKH60_03290"
     CDS             complement(4550..5911)
                     /locus_tag="PKH60_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17101.1"
                     /translation="MIDSAFAPTSSGVGTDYSSASVPISSQPGVVPPAPPAVPPAYSN
                     PTPPAKKKSSGSFFKLFGLGVVLVVLIGGLITAYVLSQQQQEIRQQASQADQYTQNPF
                     TFQDIQNLDLTFIPKPGEPEEAPSQVKGKFVFENNPSCNANMSCTTNVKFEITEINTG
                     MTMRCGTNTANLVHYTFLTNTSDPNYPGNLTHNTPTVNANCNRPVSQCGDDPNCSSSE
                     VVRLYGPSGCGDECKVFQGSLSFSVTNTEYGCGCVQFDPNISELSINCRPIDGTTYRT
                     YTYTRQTTDGPWSYASNTAQCSTPVSPSPTATITPTPTTPVGPTNTPTPTATLPPGVP
                     SATPTPSGRPTATPTPPPFGGMCLSISMSPATPAYNDSVTFTCGLVQGVAKYEFRYFE
                     PGVLTPTKLDASSGNVSKAIKVTKAGTYTAQCRICPPVTSSNTYCNDPNWGWDPTITT
                     QSR"
     gene            complement(5961..7244)
                     /locus_tag="PKH60_03295"
     CDS             complement(5961..7244)
                     /locus_tag="PKH60_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNQ17102.1"
                     /translation="MKSYHNLKTPKIANVANKTVLVRVDYNVPLESQHSTIVVKDDKR
                     IAQSVETIHFLIKAGAKIVLLSHLGRPKSAGDTHLSLAPVAQHLQKKFHIPCTFIPET
                     VGENVVATIAQLQPGEVVLLENLRFHSEEKAADTTFAKQLASLGDVYINEAFSSLHRK
                     HASTYVLPQLLPNFAGFDLAREVATLNKLLTEPRRPFICLIGGAKISDKVDAIKVLAS
                     KADAILIGGGIANNFFKAEGLEIYRSFTQESNVVTNDGKVDYSQMAGQLIAEHKHERV
                     LKDGYIPLPKIIYPIDVVAAPSITETDRRKVQMLDLTHDMLDKPEHEKLVYADIGPKT
                     VRLYRELLLQAETIFWNGPMGVWENKLFADGTHKLACAVADAAATSVIGGGDTIAAID
                     QFGLGAAYSYISTGGGASLEFLSGTELPGLEVLTK"
     gene            7323..8453
                     /locus_tag="PKH60_03300"
     CDS             7323..8453
                     /locus_tag="PKH60_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNQ17103.1"
                     /translation="MTVTTRKTNASPKVASHVTMDDLLAMPENTVVVAKKGETIIGTV
                     TGIDNKAVRVDIGSKTEGLVTEREYDLARDYIGELKIGDKVEVTVFSTENSQGQVLLS
                     LKHAALDARWDYFAETMEKSEVLEAKGVEVNKGGLIVVVNGVRGFVPSSQFGKELVGK
                     FQTLKGKSFKVKAIEVDREKNRLIFSERHVSEEKELAQKEQAISSLETGKIYEGVVSG
                     VMHFGLFVTVEVPIEDKENVGHVEGLVHISEISWEKVNHPKDFHQVGDRIQVKVLGQD
                     ERTGKLNLSIKQLKDDPWIGIDTKYPVGTTLTGKVSRVESFGVFVNIDQGIDGLIHNS
                     KLESSHNFKKGDDVTVYVESVSPDQKRMSLSPVMTERPVEYR"
     gene            8485..8652
                     /locus_tag="PKH60_03305"
     CDS             8485..8652
                     /locus_tag="PKH60_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17104.1"
                     /translation="MAKQRMIVTDDQLLIDMDLLFNAIREADGDWKKVESEMKAYLEA
                     VTGMVDSGHDD"
     gene            8720..9334
                     /locus_tag="PKH60_03310"
     CDS             8720..9334
                     /locus_tag="PKH60_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17105.1"
                     /translation="MTNAASQPFVITCGDEGVQINVGRRLGFVGAGFRLEGFDQVVPV
                     LNKLFGETMAICSSEENDWAKQKLSLVEWGQVNASMQQHIEALADQLKINYAGYLPFS
                     DPKQLKYDVKGHMVRPHKVHIANKISFTCGGGEQIYNLGNYVISADWVHLVPKKLAKA
                     LITDQVAFYQSLAGKTQLNFVFETNGVLGEKIALKNQKIVESVL"
     gene            9485..10351
                     /locus_tag="PKH60_03315"
     CDS             9485..10351
                     /locus_tag="PKH60_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17106.1"
                     /translation="MSEPNKIVEKTQEERLHEATQYVERAKVEQVDSVEQQCIDGRTE
                     SSDLLSADDKKEVMLRPAGAAGWSLILLALGYTPDLAYRSVFDFLQTKNMRYGWHTDD
                     YSQHDNHDDSVVGSSTSHRSISLKGKHKESFVLINTSKQWGILPSVEGVSAFVYDQGL
                     DDELLAEFVKWFNSSKYAEAQELNLDAVINVRNMQDTATLRLLAFDKDVYSVDFDEVG
                     TPTLSLQFKVEQHSQDELTKITSEKQQGVTGCGHLQRAINYCKAYGVNQNDVTTLYQL
                     IQAETAASITYN"
     gene            complement(10365..>10589)
                     /locus_tag="PKH60_03320"
     CDS             complement(10365..>10589)
                     /locus_tag="PKH60_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17107.1"
                     /translation="SGQIQVLSTNDTATEKQFVGSGSFIGCGGVELDREFAASVNDDY
                     ASEVFLFNPTLVRNFPTALKDAHVTWSEGI"
BASE COUNT         2983 a         2020 c         2470 g         3116 t
ORIGIN      
        1 tcgaattgct tttgaagtgt ggcttgatca atcgttgcaa ttgtttgcca ttttttgaca
       61 ggtgagcctt cttttgcacc cagatacgtg attttacctc cagctaaaaa gcgcaggtta
      121 gctttttctt tagcatcaac aatgccagat acatcgaggg ttttaactag ggtttttctt
      181 tctgggttgg tagttgtaat ttctggtttg gctgcttgct gtttttgaaa ataaaagaat
      241 gcgccacctc ccagaacaag tagtagaacc agaatcaact tccaacgttt cttaagtgtc
      301 gaaagtaatt gtgttttcaa tttaaaaatt ctgcttttca tagatttaag aagtctattt
      361 taccatgagt tacggtataa tactctggct tgcaatagta cgagccagtg cgtgtgtagc
      421 tcaatcggtt agagcaccaa gcttatacct tggtggttcc aggttcgagt cctggcacac
      481 gcactttgtg acaggacgag aagtttccct agtcgtgaca acgaccagga aatcctgtaa
      541 ggacaagcaa gtgagcaaag ctcacctgct tgtcccgcat tagcgggaag tcctggcaca
      601 cgcaccacac gacagacgca tgagatatta tttggttttc ggaaacctga ggcgtgaata
      661 atttctctca agcttattaa tagtccaatt tattgagtca caatataacc ctctttgccg
      721 accatgtatg gtaaagcgta ttttaaaagt aaagcgatta tttaaaagta aagtcgctct
      781 atggtaacta tagtggcata attggtacca gaccgatggg gttacactgg cttttggtat
      841 actttctcta tgtccactta ttatttagtt cgtcatggtt taagagtaag tagggatgag
      901 gatacccttc taagtgatat gggtattaga caagccgaac taactggtga atatctacag
      961 tcaaaaaaca ttcaccatat ttttgtcagc cccgtaaaac gaacacttca cactgcagag
     1021 attatctata gctatctcca agtaccacaa tcaactgacg cgcggttaaa agaacgaatg
     1081 gaatatgagc caaagcaagg tagttttgaa tcgtttttag gtgagtggga taaaacgatg
     1141 gccgatcgta tgtatcagcc gccgtacgga gattcagcag ttcatgcggg agatagaatt
     1201 gtcagcttac ttgaagaaat aaacgatgat aacaattatg tttgtatttc acatggcgga
     1261 gttattggtg atgtagtaag aaatttattt ccagagaggt tgtatgcgct taaagccgac
     1321 ccaataaaac cattgaaatg gttgaacatt ccagaagctt cggttacaga gattcagaaa
     1381 attggggaca cttacaccct aaaaatggtt gggaatatct cgcacctcac tcgcaaataa
     1441 aacatagttt agatcgccag ccagccacga aaacctctag aactatagta ggaagacgcc
     1501 ccattgtggt acacaaaaac ggtgttataa cgtcgatcag cgaacagtgc tccaccgagt
     1561 tttctgattt ccgcaggtgt tttgatccaa cttgatgttt tagtgtcaaa ctcgccaagt
     1621 tgctgtagtt ttttatattg tgtttcggtc agcagttcaa tacccatatc ggttgacatg
     1681 ccaagagcgc tcagttcggg tttatgttct ttccttgagt ctagtgcctc cttgtcataa
     1741 caacagcttc tgcggccact tggactttct acagcacagt caatgaatag atattcaccg
     1801 gtggtattat cttgacccac aacatctggt tcaccaccgg ttctttccat ttcattgaga
     1861 gaccaaagtt ttgttgggtt gatcttcaat tttgctttga ttttttccca ctttaaatct
     1921 gggtgcctat ctgggttttg attaaaccgt ttctcgagaa ccatcagcaa ctgatcgccg
     1981 actttaggaa gtaaagcttt aaggactttc ataataataa tacttactag agttcgctta
     2041 agctaatttc aaaaagggga ttggtgatat catcaaattc atctactaaa acaacagatg
     2101 tgtcggtcaa acggattgct ttagcaatgc cttttgcatc atctaggtct cgttcaaaga
     2161 caaaggggtt gagagtctca gtactgaggt caccttctgg tggaacatct actagtaatg
     2221 gttcaagact tggtacgtca tacaaccgca agctttgttt accactgatt ccttgactat
     2281 atagtacgag caatcgattg ctgggtaaaa ataccatatc tagcacggat tcagtaccaa
     2341 aatcatccaa agtgtaaacc cgatcgttgg atagttcata taatgacact actgcgtcaa
     2401 tacctgaact tgataggtcc tcaaaagcaa cgtagtttcc agaaggagaa agtgagctta
     2461 cggtgtattt ttcacatttg gcgatcgcag ggtcaaatgc tgttggtaaa gttttcatgt
     2521 tgccattaat tgttagctgg acagtgcagt cagcaagcat agcaacctgg gtgatttttg
     2581 gttcagaaag ggcaagattc gtggtttgat ccgattgatt cccctcaggg ttgtcatttt
     2641 gctgtaaaaa cagcaacccg ccagctacaa caataagaaa aatgccaatc aaaaaaaaac
     2701 ttcgcgacat atatttactc aacaacgtac tttgtgttta aagcattagt agaagggcag
     2761 cgccaattcc caggatggcc agaccagctt tgagccagtt accccaatct tctgggccgg
     2821 tttctggtaa agtttctggc aatgctggtt gttgcgccac ctcagctggt tcggttaccg
     2881 ctgcaactgg gttttgacaa ttcatgtcca ttggattgga ttccaacctg caacgatttt
     2941 ccgtttcatt gactacaaag cagatgtgag ctggattact acagtcagca ttcgtgacac
     3001 aaattgcatt acatccaata accggtgtta ctctagtggt tggtgtagca gttgctccgg
     3061 gaacagtggt aggtgttgga gtaggcgtag agcttggatt ggtggttggt gttgcagtcg
     3121 cactggggtt gggagtagct gttggtcctg taatggtgaa ttggagtgta cattgaccaa
     3181 ctggaggtcc accaattgct cctggagcta atgcccattg gttgtagcat tgtggagctc
     3241 cgtcagccga ataattaatg gccctgatga ggaagcgata cgagggcaaa ttttgatatt
     3301 tttctggaag agagagacga agactcttag aatttggatc gacattgtct gctaatagag
     3361 caatatagta tcgatcattt ccaatctctt caggcgtgcc ttctgcaatg agccagattt
     3421 gatatttttg ttgtggtgct tggacaggat tccattcaaa aagagcagta tttttatctg
     3481 gcatagtgac ctttcggtca agcacaacgg gaggtaagca gagtgcccat tgatcgtaac
     3541 aggctggagc tcgctgggca tttctttgag aaacagcacg aatgaggaat cttctagttt
     3601 ttagagtatt tgggttagca actgtatatg aagtagcagt gcgtggtagt ggttgggttg
     3661 tgagattgct taagtaatat ttttctggag cttgttcgtt gagtggttcg taactgatat
     3721 tccaaatttc ataatcggta acctctccgc tggtaagttt ccagctgagt tgaattttgg
     3781 aattgttttc aatccaattg acctgtctgt catctacaac cggtctattt tcagtacagg
     3841 ctcctacaaa agtactggtt ggagtgggag tggcggcagg actggtatac gtaccgattg
     3901 cagcctgaga agtacaactt gtgcctgtct tgaaagcaaa tactaaaaat ctgtaatacg
     3961 gtcgctctct aactactttc ccttccaaat caatcacatc gttggtaggt aaggtccaga
     4021 catactctgt ttgagtggga gagagagatt ttgcatttgc aggtacaatt ggtgcgaatt
     4081 gaggagtttc gctcacttca gaagaattat caagtacgtt gaaaataata tatttccccg
     4141 atggatgagt tcccgcttct ccctctgtaa ttagaaagat gcgataaccg cttgcaccag
     4201 caacttggtt ccactgaagt ttaaattgag tatcgttgac tttcgtaaca gcagggttgc
     4261 gagccagcgg agcgtccata caagcgtcag ttgatgcttg atttctagta tcttgttctt
     4321 cttgggataa aaagagagct actccgccac cagctagcaa cacaaacatc attagtccag
     4381 ccatggcaag tttaaagttc tttttcatct gattaatgcc attgggcttg gttgtctgca
     4441 tcacggcagc ttgtgttggg gatggtggtt gaggaccagc actcaccggc tgaagagtag
     4501 gtggtagctg ggttgagttg gtagtttgag ttgttgcgtt taccatagtt tatcttgatt
     4561 gcgttgtgat agttggatcc caaccccaat ttggatcgtt acaataggtg tttgagctag
     4621 taaccggagg acagattcgg cattgggctg tgtaagtacc agccttcgtg actttgatag
     4681 cttttgaaac attgccagaa cttgcatcca attttgtggg cgttaacacc cctggttcga
     4741 aatagcggaa ttcatatttt gctacacctt gaaccagacc gcaggtgaaa gtcacagagt
     4801 cgttatatgc aggggtagca ggactcatac tgatgcttag acacatgcct ccaaagggag
     4861 gtggtgtggg cgtagcggtt ggtcgaccac ttggcgtagg agtggcgcta ggcacaccag
     4921 gtggaagtgt agctgtcggt gttggagtgt tagttggtcc aactggagtg gttggtgtgg
     4981 gcgtaatggt ggccgttggt gaaggactca ctggcgtgga acactgcgcc gtatttgacg
     5041 catatgacca tggtccatcg gtagtttgtc gagtataggt gtaagttcga tatgtagttc
     5101 catcgattgg cctacaatta attgaaagct ccgaaatatt tgggtcaaat tgaacgcagc
     5161 cacaaccata ctcagtattt gtgacagaaa acgacagaga tccttgaaag actttgcact
     5221 catcaccaca accggagggg ccatagagcc gaactacttc agaactagaa caatttggat
     5281 cgtctccgca ttgacttact ggtctattac aatttgcatt cactgtggga gtattgtgtg
     5341 ttaggttgcc tggataatta ggatcgctcg tgttagtgag aaatgtataa tgcaccagat
     5401 tggcagtgtt ggttccacat cgcattgtca ttcctgtatt tatttcagtt atttcaaact
     5461 taacattggt ggtacaactc atgttggcat tgcaacttgg gttattttca aagacgaatt
     5521 tgcctttaac ctgcgatgga gcttcctcgg gctcgccagg ttttggaata aaagttaaat
     5581 caagattttg gatatcctga aacgtaaatg gattttgagt gtactgatca gcttgactcg
     5641 cttgctggcg gatttcttgt tgttgttgag acaaaacata ggcggtgatc agaccgccaa
     5701 tgagtactac caagaccact cctagaccaa aaagtttaaa gaaagaacca gaactttttt
     5761 tctttgcagg gggagttggg ttgctatacg ctggaggaac tgcggggggt gctggaggta
     5821 ctactcctgg ttgagatgaa ataggaacag aagcgctgga ataatcagta cccacaccag
     5881 aactggtagg cgcaaacgca gaatctatca tctgttacta gaatactcac gtttccaccc
     5941 atgcgtcaag ggttgattat ttattttgtt agaacttcta agccaggcaa ctcagttccg
     6001 ctaagaaact cgagggaggc gccgccgcca gtagaaatat atgaataggc ggcgcctaaa
     6061 ccaaattggt ctatggcggc gatggtatcg ccgccaccga ttaccgatgt tgcggcggca
     6121 tccgccaccg cacaagctaa tttatgagtg ccatcagcaa ataacttatt ttcccaaacc
     6181 cccattggtc cattccaaaa aattgtttct gcctgcaata atagctctcg gtacagtcga
     6241 acagtttttg gcccaatatc ggcataaacc aatttttcgt gctcaggttt atccaacatg
     6301 tcgtgggtca aatctagcat ttgtactttt cttcgatcag tttcggttat actgggagcg
     6361 gccacaacat caataggata gataattttg ggaagtggaa tgtacccgtc tttgagcacc
     6421 cgttcgtgtt tgtgttcggc gatcaactga ccggccatct gactgtagtc cacttttcca
     6481 tcattagtta ctacatttga ttcttgagta aacgaacgat aaatttctaa accctctgct
     6541 ttaaagaagt tattggcgat acctccacca atcagaatgg catctgcttt tgaggcgagt
     6601 acttttattg catcaacttt atctgaaatt ttagctccac caattagaca aatgaatggt
     6661 cttcttggct cagtgagtag tttgttcaaa gttgcgactt ctctggccaa atcaaatcca
     6721 gcaaaattgg gtagtaactg tggtagtaca tacgttgaag cgtgtttgcg atgtaatgag
     6781 gagaaagctt cattaatata gacatcgccc aaactggcta attgtttagc aaatgttgta
     6841 tcagcagctt tttcttccga gtgaaatcgt aaattttcaa gcaatactac ttcgccaggc
     6901 tgtaattgag cgatggtagc tacaacgttc tctccaacag tttcaggaat aaatgtgcac
     6961 ggaatatgaa actttttttg caaatgttga gcaaccggtg ccaaagagag atgagtatcg
     7021 cctgcgctct tgggtcgccc caaatgcgac aacaatacta tcttggcacc tgctttaatt
     7081 aaaaaatgaa tggtttcaac cgattgagca attcttttgt catctttgac aacaatcgta
     7141 gaatgctgac tttcaagcgg gacgttgtaa tctaccctca ctaatactgt cttatttgcc
     7201 acattggcga tttttggtgt ttttagattg tgatagcttt tcatgcgcaa tcctccagag
     7261 aaatttactg ttgtgtatgg tatactccct cgcactcgtt taagaaaggg gatatatata
     7321 gtatgacagt aacaactcgt aaaaccaatg cgagtcctaa ggtggcttct cacgtaacaa
     7381 tggatgatct tctggcaatg ccggagaaca ccgtggtggt tgccaaaaaa ggagagacaa
     7441 ttatcggtac agttaccgga attgataata aggccgttcg ggtcgatatt ggttcaaaaa
     7501 ctgagggttt agtaacagaa agagagtatg atctcgcccg agattatatt ggtgaactaa
     7561 aaatcggaga caaagttgaa gtcacggttt tttccacaga aaatagtcaa ggtcaggtac
     7621 tgctttcatt gaagcacgct gcccttgatg cccgatggga ttactttgct gaaaccatgg
     7681 agaaatctga ggtactagaa gcaaaaggtg tggaagtaaa taaaggtggg ttaattgtgg
     7741 ttgtgaatgg agtgcgcgga ttcgtgcctt caagccaatt tggtaaagaa ctcgtgggca
     7801 aattccaaac ccttaaggga aaatctttca aagttaaagc gattgaagtt gatagagaaa
     7861 aaaatcgctt aattttttct gagcgtcatg tttctgaaga aaaggagttg gctcaaaagg
     7921 agcaggcaat tagctcactt gaaaccggta agatttatga aggtgtggtt tctggcgtga
     7981 tgcacttcgg attatttgtg acagtggaag tgccaattga agacaaagaa aacgtgggtc
     8041 acgttgaggg attggtccat atttccgaga tttcttggga aaaagttaat catccaaaag
     8101 attttcacca agttggagat cggattcaag tgaaggtact cggccaagat gagcgcacgg
     8161 gtaaactcaa cctttcgatc aagcaactaa aagatgatcc ttggattggg atagatacca
     8221 agtatcccgt tggaaccacg ttaactggaa aagtttctcg cgttgaatca tttggtgtat
     8281 ttgtgaacat tgatcaagga atagatggac tcatccacaa ttccaagttg gaatcaagtc
     8341 ataacttcaa gaagggtgat gacgtcacag tgtatgtgga gagcgtcagt cctgatcaaa
     8401 aacgcatgag cttgagtcca gtgatgaccg agcgcccagt tgaataccgt taatatatag
     8461 tacaaagcca aaaaagggag attcatggca aaacaaagaa tgattgtcac cgatgaccaa
     8521 ttactaattg acatggattt gttgtttaac gctattcgtg aggctgatgg tgactggaaa
     8581 aaggttgagt cagaaatgaa ggcctacctc gaggcagtga ctggcatggt tgattctggc
     8641 cacgacgatt aagagtaact attatactca gactgctttt ttagcaagat tattcgtggt
     8701 ataatgatcg ggttaattta tgacaaatgc cgcttcccag ccgtttgtga tcacctgtgg
     8761 tgatgaagga gttcaaatta atgttggacg ccgattgggt tttgttggcg caggatttcg
     8821 cttggaaggt ttcgatcaag tagttcccgt gctcaataag ctttttggtg aaacgatggc
     8881 aatttgttcc agtgaggaaa atgattgggc gaaacaaaaa ttatcgcttg tggagtgggg
     8941 gcaagtgaat gctagcatgc aacaacatat tgaggcattg gctgaccaac taaaaataaa
     9001 ctatgctggc tatctgcctt tctccgaccc aaaacaatta aagtatgatg tgaagggtca
     9061 tatggttcgc cctcacaaag ttcatatagc aaacaaaatt tcttttacct gtggtggtgg
     9121 agagcaaatt tacaatttgg gcaactatgt tatttcagcc gactgggtgc atttggtacc
     9181 aaagaaactg gctaaagcct tgattacaga ccaagtggca ttttaccaat cgttagctgg
     9241 caaaactcag ctaaattttg tgtttgaaac caatggtgta ttgggagaaa aaattgcact
     9301 taagaatcag aagattgttg aatcagttct ctagttttca attctcatgt ctggtattta
     9361 tttcttgatc attttgcagt ggtgctgata taattccgcc ttcagaaatt attagatcga
     9421 tgaaaagtgg ttagcgagca accataagaa agtatttatg catgatcaag agaaaggaat
     9481 ttatatgtca gagcctaaca aaattgtaga aaaaactcaa gaagaaagat tgcacgaagc
     9541 aactcaatat gtggaaagag ccaaagttga gcaagttgac tcggttgagc aacagtgtat
     9601 tgatggcagg acagagtcat ccgacttatt gagtgctgat gataaaaagg aagtaatgct
     9661 ccgtccagca ggtgctgctg gatggtccct aattttgctt gcacttggat atactccaga
     9721 tttagcgtat cgatcagtat ttgacttttt acaaacaaaa aatatgcgct atggttggca
     9781 cactgacgat tattctcaac atgataacca tgatgatagt gtggtaggta gtagtacttc
     9841 acatcgatct atttctctta aaggaaaaca caaagaaagc tttgtcttaa tcaatactag
     9901 taagcaatgg ggtattttgc cctcagtaga aggagtcagt gcatttgtgt atgatcaggg
     9961 tttagacgat gagttgttag ctgagtttgt aaagtggttt aatagttcaa aatatgcaga
    10021 ggcacaggag ctgaatctag atgcagttat caacgtgaga aatatgcaag ataccgcaac
    10081 cctaagattg ttagcttttg ataaggatgt ttattcagtt gattttgatg aagttgggac
    10141 accaacgctg tctttacagt ttaaagttga gcagcattct caggacgaat tgactaaaat
    10201 tacctcagaa aaacagcagg gtgtaactgg ttgtggtcat ttgcaaagag cgattaatta
    10261 ttgtaaagca tatggcgtca accaaaatga tgtcaccacg ctttatcagc taattcaagc
    10321 agagacagcg gcaagtatta cctacaatta gttttgctga ctttttaaat tccctcactc
    10381 catgtcacgt gagcatcttt taatgccgtt gggaagtttc tgaccaaggt tggattgaag
    10441 aggaaaactt ccgacgcata gtcgtcattg actgaagctg caaattctcg atccagttct
    10501 actcctccac agccaatgaa acttcctgat ccaacaaact gtttttctgt ggcagtatca
    10561 ttggtagaga ggacttggat ttgtccgct
//
LOCUS       DAOQZP010000034        10503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_6790_length_10503_cov_5.384763, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000034 DAOQZP010000000
VERSION     DAOQZP010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 10503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10503
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6790_length_10503_cov_5.384763"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1610)
                     /locus_tag="PKH60_03325"
     CDS             complement(<1..1610)
                     /locus_tag="PKH60_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17108.1"
                     /translation="MKFFKRLQKNGVSPKTFLSFLGFLILSMGVLAGIALLDTNSENR
                     SRANIYTSYTCSTPDGSISVELTATHSETVGTNSVTATGGISKPAGFYYDVIANLYRY
                     ACTCPDVNQGVCPSCTSSSIPVTNSGGTSITTIWNESNAQFPATTGCATLQSDVTLGG
                     VLLRNPNTGNTFSVSCVPAPGPGISISEVSGYFCAAASPTATPTPTSTPTATPTIAVN
                     TPTPTAPPTIPTNTPTPIVVTLSCEQCDLDQDNRVEHTTPTRDDLDFIIGCLGQSSTS
                     SPCNRANLLPGGPLINSGDINVFVSQCTSVYSNNQTCSAVNTPTPTPTLEATASWWQS
                     YSGLIYAQSMIKSILQPSFNLVANMTSNLTNSAGVPLNNTNGIYLGAGGPSAHSSLRQ
                     VTGQQSTVCAEYNIPKLLEMANAQIITPSIRGLITNSGEFMSGTGYSDFGTYRVFRHS
                     GDLEIDLNSTWNVVGTPFIIFNEGGTITIANSANNENIITVANDSFLGLFTSGSIVIE
                     DTVGSSTPRSGASLSPALTGLFFTNDPTG"
     gene            1839..1912
                     /locus_tag="PKH60_03330"
     tRNA            1839..1912
                     /locus_tag="PKH60_03330"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1873..1875,aa:Ala,seq:tgc)
     gene            2092..5632
                     /locus_tag="PKH60_03335"
     rRNA            2092..5632
                     /locus_tag="PKH60_03335"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     assembly_gap    2585..2684
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5728..5844
                     /gene="rrf"
                     /locus_tag="PKH60_03340"
     rRNA            5728..5844
                     /gene="rrf"
                     /locus_tag="PKH60_03340"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            5897..6040
                     /locus_tag="PKH60_03345"
     CDS             5897..6040
                     /locus_tag="PKH60_03345"
                     /inference="COORDINATES: protein motif:HMM:NF039953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HNQ17109.1"
                     /translation="MFKELFEYLWSNKKWWLIPPVIIFILFGAMIIFSSASPVSPFVY
                     MLF"
     gene            6278..7987
                     /locus_tag="PKH60_03350"
     CDS             6278..7987
                     /locus_tag="PKH60_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015784292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HNQ17110.1"
                     /translation="MYILGISCYYHDSAACLLKDGKVVAAGAEERFTRKKHDNSFPIN
                     AITYCLDFAGIAMNEVDEVVFYEKPILKYDRVLNQYLDTFPKCYSTCKDTISQWFDHK
                     LQISQKLKEELFYLKPVLYVGHHLSHAASAYYLSGFKKSVILTLDGVGEWATTTIGTG
                     IDGELKIDKEIQFPHSLGLLYSAMTTYLGFSANDAEYKVMGLAAYGNVKPFLKHFDQL
                     ITQFDDGSYQLNMEYFTFDWSNRSMYSSKLIELFGHPARKPESKMMSYHKNIAASLQQ
                     KLEEVVLKMLNVAYETYKTPNLCLAGGVALNSVLNGKILSKTKFKNLYIPPDPSDAGG
                     AMGAAFYVYHQKIHKAIDHKSFNPYLGPSYSWFQIKKVLDAQQIKYTFYESRDDLTTN
                     VAQLVQKQNIIGWFQGRMEWGPRALGNRSILASAATTKMRDIINEKVKHREMFRPFAP
                     VILEEHIEDYFVADKNLPISARYMLMVYPFKPKGEKDVPAVVHVDGSGRLQTLVRSDN
                     PLYYELIEKYYQLTGVPIIINTSFNVRGEPIVCSPEDAVNCFLKTDIDYLVIDQYVCR
                     KKD"
     gene            7969..9000
                     /locus_tag="PKH60_03355"
     CDS             7969..9000
                     /locus_tag="PKH60_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17111.1"
                     /translation="MPKKRLALVLLVFSEIIAGLFFLFFVHLHFTNKNEIEQEKKVAL
                     VSSEFTQLHNSDRFQYYTELRPNLSEKNQPSWLSHEAVYTYNSDGLNDRYDYSLEKDK
                     NVFRIIALGDSFTFGHYVNTEDSWPEQLEDILNQDELNHSEISFEVINLGMRGFDIPY
                     SVERYKRIGQKYSPDLIIWFESGSGFQRYVELLWPIIFSCVDNNEKNAADAISLEKLK
                     IDYQHLVKCWISGEEFLIQKYSQKTIETKLDEFLDEFYLLTENTPTTFILFDSSSLNQ
                     VQKERVSYLKNRYKRFSFETIVQELKTEEVLPDGHPSREGHEKIAQSIHHYLISSSSL
                     PSSTCEGDQ"
     gene            9042..9998
                     /locus_tag="PKH60_03360"
     CDS             9042..9998
                     /locus_tag="PKH60_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNQ17112.1"
                     /translation="MGASFLGYYYKETRVSMRSFDQRKVAIINRGGVEKKPSPNFLYY
                     SELVSNNIIESQLPWDSGVEIKYKINNDGLNDRKNYDLVKPKNTFRIITLGDSFTFGH
                     YVSTDNNWPEQLEELLNTKEVCPSTTIEVINLGMWGFDINYLIKRYKDIGKKYEPDMV
                     VWFESGSGFTRLVETMEPLSVECEKQIENFVSLDVLKRIKYSGECWGQSQHKVVVDLG
                     EDGLKRVMTTYLDDLFTILDQSIFFYVTFEKIESGINAESTLNFWKNRYDKVHFSKIP
                     NLKQGELFPDGHPSTLGHKKIAESIFELVKESNSQELCITEN"
     gene            complement(10030..>10503)
                     /locus_tag="PKH60_03365"
     CDS             complement(10030..>10503)
                     /locus_tag="PKH60_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17113.1"
                     /translation="QLVDKWIKFEIAQNYYLKDVGDYYSYSNLESQMITNRELAKAVN
                     VGLVLFHRPKDDLVVNESIHESIKTIKTVQKQRVIGGWNEYPYWYLDQTIWANLLQKT
                     KAKTFPDSYTVYAELEWKKGNLNRNMTAIHFSGPFKKHLSLYQKEISKKYSTYFE"
BASE COUNT         3162 a         1881 c         2383 g         2977 t
ORIGIN      
        1 ccggtgggat cgttagtaaa gaataatcct gtcaaagctg ggcttaagct tgccccagac
       61 cgaggtgtgc tggagccgac ggtatcctcg atcactattg agccactggt aaacagtccc
      121 aaaaatgagt cattcgcaac agtgatgatg ttttcattgt tggcagagtt ggcaatagtt
      181 attgttccac cttcattaaa aatgatgaat ggtgttccaa caacattcca agttgaattg
      241 agatcaattt ctaggtcgcc actatgccga aatacacgat aggtaccgaa atcggaatag
      301 ccagtgccac tcataaattc tcctgagttg gtgataagac ctcttatgct aggtgtaata
      361 atttgagcat tggccatctc tagtaattta ggaatgttgt actctgcgca aacagtgctt
      421 tgttgaccag ttacttgacg taagctacta tgcgctgatg gtccgcctgc tcctaagtaa
      481 attccattgg tattattaag tggaacgcca gcgctgttgg ttagatttga agtcatattc
      541 gcaactaaat taaagctggg ttgcaaaata gacttaatca tactttgggc ataaattaaa
      601 cctgagtatg actgccacca tgaggctgtt gcttcaagtg ttggggtggg agtaggggtg
      661 ttaacagcag agcaggtttg gttgtttgaa taaacactag tgcactgtga aacgaataca
      721 ttaatgtctc cagaattaat taatggacca ccgggaagaa gattggctct attgcacggt
      781 gatgaagtag aggattgacc taagcatcca atgataaagt caaggtcatc tcttgttggg
      841 gttgtgtgtt ccacacgatt atcttgatct agatcgcact gttcacaact caacgtcaca
      901 acaatggggg ttggggtatt tgttggtatg gtgggtggag cggtaggtgt gggagtatta
      961 acagctatgg ttggcgttgc agtcggggtg gaggtaggtg ttggagttgc tgtgggtgag
     1021 gctgcagcgc aaaaataccc cgatacttca ctaatactaa ttcctggtcc tggagcagga
     1081 acacagctga cgctaaaggt gttgccagta tttgggtttc tcaggagtac accaccgagg
     1141 gttacgtcac tttgtagtgt ggcgcagcca gtggtggcag gaaattgcgc attgctttca
     1201 ttccagattg tagtaattga tgtgccacca ctatttgtaa cggggattga tgaggaggtg
     1261 caagatggac aaactccctg gtttacgtct ggacacgtac aagcatatcg gtacaaattg
     1321 gcgattacat cataatagaa tccagctggt ttggaaatgc ctccagttgc ggtcacagaa
     1381 ttagtgccga cagtctcact atgagttgcg gttaactcta cagaaatact tccatcaggt
     1441 gtactacagg tatagctggt ataaatattg gctctagagc gattttctga gttggtatcc
     1501 agtaaagcaa tgccggctag tactcccatg ctcaagatta aaaatcccaa aaatgagaga
     1561 aatgtttttg gagatacccc atttttttgt aatcttttga agaatttcat agttgagtta
     1621 agtctataga caaatctgct atagtgcaag ctttccttgt atgaggttaa tcaacgccca
     1681 attgacccag attgataact tgacataaat cagggtcttt ggtactatac taaccctgtt
     1741 cgggaaacag gcacattaga gttgatattc tttacactca ttacttcgag acagtaaaga
     1801 atccctcaca aaatttaata catcgaagaa agagatttgg gctcgtagct cagctggcta
     1861 gagcgcccgc cttgcacgcg ggaggtcagg agttcgaatc tcctcgggtc cactcaggtg
     1921 gttattcttt attagttctt ggttgagctg atcggctaaa actaggagct aacaaagagg
     1981 aactcctctg gatgctcatt aacaagtgaa gaatacatgc gcagtcatcc ccatgactgt
     2041 gcatttggtc tcatcttcca taacataata atgttaggaa aatcatctca aagtatgtga
     2101 attaatgcag atagtggatg cctatggctt gtttagccga agaaggacgc actaggccgc
     2161 gatagtcgtc ggggagctgc caagaagctc tgatccggcg gtgtccgaat agagaaatct
     2221 tccttcgtga aagcgaaggg atcctcccgt ttacgggagg agggtaagtc ggggaactga
     2281 aatatctaag tacccggagg aaaaaaaatc gaatgagatt cccttagtaa gcgacgagcg
     2341 aacagggatc agcccaaacc ctcaatttat tgggggggtt gtaggacctc aatattccaa
     2401 gatagtcgat agaaaaatgc tttggaataa gcaaccaaag aaagtgaaag tcttgtattc
     2461 gaaatcgact gttacggata gagtgttcct gagtaggttg ggagacggca aactcccttc
     2521 tgaagcaggg gtgaccacac tccaaggcta aatactaaac aagaccgata gtgaacgagt
     2581 accgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagaccc gaaaccaggt
     2701 gagctatcca tgaccagggt gagtcccgat gaaaatcggg aggaggcccg aaccggtgtt
     2761 tgctgcaaga agctcggatg agttgtggtt agaggtaata tgccaatcga acctggagat
     2821 agctggttct ccctgaaata tatgtaggta tagggtcacg ctacaagcgc agaggggtag
     2881 agcactgatc gggtgtttcc gacgaaagtt ggaacatcta gtcaaactcc gaatactctg
     2941 tgttattccg tggcactcag cacttgggag ctaagttcca agagcaaaag ggtaacaatc
     3001 cagacctaca gctaaggtct ctaatattcg tttagtggga aaggaagtgt cacgcctatg
     3061 acacctagga ggtcggctca gaagcagcca tcctttaaag aaagcgtaac agctcactag
     3121 ccgtaggcgt aatgcgccga aaatgtaacg aggctcaaac gaataaccga agcttaggat
     3181 tctctttccg atttatcgga aggggagtgg taagggagcg ttctgtgtgc agtgaaggtc
     3241 cgccgaaagg acgggctgga gcgatcagaa gtgagaatgc cggcataagt aacgaaattg
     3301 aggtgagaat ccttaacccc gaatgaccaa ggtttccccc gccatgttcg tcagcggggg
     3361 gttaggcgac cctaaggcaa gcccatcttt gatggggtaa ccgatggatt actggttaat
     3421 attccagtcc ttggcactat tcgtttgacc tggggaggga cggtgcagga tagcttgtgt
     3481 ccaaatgtga attttggatc taagcagtaa gagagtctgg ataggcaaat ccgttcagac
     3541 gcgttttacg caagtagagc acactcgagc tgtgatggca ctctaattct tcggaaaagg
     3601 agtgacaagt gagttcagca tcctggaaaa gcttcgcagg gagaatagtg tcaaatcgta
     3661 ccgataaccg acacaggtgg tcaggtcgag tagaccaagg ggagcgagag aaagtagttt
     3721 aaggaattcg gcaaaacagc tgggcgtaat ttcgagagat gcccttcccg taccgtcatt
     3781 ggtacggggc gcagcgaaag ttatgaggtc gactgtttac caaaaacaca ggtccgtgca
     3841 aactcgtaag aggaagtata cgggctgaag cctgaccggt gccggaaggt taattgcgag
     3901 ggtttttccg atttatcgga aaggccttca atcgaagccc cggtgaacgt cagcagtaac
     3961 tataactgtt ctaaggtaga aaatcgctgc cttagtaaaa ctttgctaaa tgctggaaaa
     4021 cctgagaatc ccatgatact atggtaacaa tattccttag aagtaagagt ctatgttacc
     4081 aagtgacaat tctatgggtg cagacaatca gcaggaaaga cttaaactaa gttgttggat
     4141 agtgggcttt acagatggag aaggttgttt ttctgtaagt atatttagaa ataaaacaag
     4201 taagttgctt tggcaggtaa tgccagagtt tgtgcttact caaggagaat cgagtttagc
     4261 gagtctaaaa atggttcaac attttttcca agtaggtcgt atctatatta ataaaaggta
     4321 tgacaatcac agagaacacc tctatcggta ttgcgtgaga aacagaaatg atttaacaaa
     4381 catcattatt ccgtttttca agaattatcc tctcaagaca aataagcaaa tggattttgt
     4441 tcgttttgtt caaataattg aaaaaatgaa tcgaaatgaa catctgacac aaagtggctt
     4501 agacgccata gcaaaaattg ttggaaagaa acttagttaa gaatcctcag agactatacg
     4561 cagagcttca gatcattctg aagaagatat agtccgaact tcttggcgac aagaagagta
     4621 caagtaagtc atttgtacac tcgaaaaaaa atcgagttaa cacatgtatc gtgaaaacga
     4681 tttgagcgaa gttccttgtc gcttaattgg cgacccgcac gaaaggtgca acgagtccca
     4741 tactgtctta aactattact cggtgaaatt gaaatggctg tgaagatgcg gcctactaac
     4801 ggcaggacaa aaagaccccg tagagcttta ctacatcttg gcactgatga tgtatgtgag
     4861 attgtttagt ataggtggga gaatccttct tcgtgttgga ttcgtcagtg aaataccact
     4921 cgttttatgt gcattatctt acttacccta ctgcaatcgt agggaagaac actgtctggt
     4981 gggtagttta actggggagg ttgcttgcta aagagtaacg caagcgcgca aaggtaccct
     5041 tagcctggat ggaaatcagg ctggacgtgt ataagcataa gggtgcttaa ctgtgagaca
     5101 tacaggtcga gcagatgcga aagcaggttt aagtgatcct cgtgtgtttt gtggaagaca
     5161 ccgggcttat cggataaaag ctaccttggg gataacagag tcatcgcacc taagcgtccc
     5221 tagcgtcggt gcggtttgct acctcgatgt cggctcatcg catcctggag ctgaagaagg
     5281 ttccaagggt ttgtctgttc gccaattaaa gcggtacgcg agctgggttc agaccgtcgt
     5341 gagacaggtc ggcctctatc cgccgttagc gttagaagtt tgagggattt tgcccctagt
     5401 acgagaggac cggggtgagg caatccctag tgttcctgtt gttgctgtca agcgcattgc
     5461 agggtagcta cattgttgat ggataatcgc tgaaagcatc taagcgagaa gccacgccca
     5521 agataaaact tctccattag accactcgga gactacgagt ttgatagacc gtcagtgcaa
     5581 gtccagtaat ggattcagct taacggtact aagtgtcgat tgaacatact ttgatagacc
     5641 aagtgtatag tcccaaaacg ggatggatac gctgtattct tcacctgtta gcgagtattc
     5701 gctacacaaa acattagacc gctgtgttct ggtctttgga gcggggtgga accaactctt
     5761 cccttcccga acagagtctt gaaacgcccc agcgctgaca atactaatcg ggcaactgat
     5821 tgggacgata ggtcaaggcc agaacagagc ggttttttgt tggcaatttt cataaactag
     5881 tgtatatact ctaactatgt ttaaagaatt atttgagtac ctttggagta ataaaaagtg
     5941 gtggctaatt ccaccagtaa ttattttcat tttattcgga gcaatgataa tattttcttc
     6001 agcttctcca gtgagtccgt ttgtttatat gttattttaa ctagaaaaaa tggttggata
     6061 tgcatgaatt aaaaatattc cttgagaaaa ttcaaggggt tgttacgaag ataatcagtg
     6121 gcatattttc taatttattt tgcgtctttg gcattggatt aacgtcgttt atggctctgt
     6181 tggtaggaaa aagattcctt cgtagaatat acaaagattc ctcttgggtg caaagtaata
     6241 gcaaagcaaa catctataag caattctagg tttttttatg tatatcttag gcatttcttg
     6301 ctactatcac gattcagcgg cttgtttgtt aaaggacggc aaagttgttg ctgctggagc
     6361 agaagaaaga ttcactagaa aaaaacacga taatagtttt ccaattaacg ccattacata
     6421 ttgtttagat tttgctggta ttgctatgaa tgaagtggac gaggtggtgt tttatgaaaa
     6481 accaattttg aaatacgatc gtgttctcaa tcagtactta gacacatttc caaagtgcta
     6541 ttcaacttgc aaagatacta tctctcagtg gtttgatcac aagttacaga tcagtcaaaa
     6601 attaaaagaa gaactatttt atctaaaacc agtattgtat gtgggtcacc atctgtcaca
     6661 cgcagccagt gcctactatc tttcgggatt caaaaagtct gtcattttga cattagatgg
     6721 tgtgggtgag tgggcaacga caacaattgg aacaggaatt gatggagaat tgaaaataga
     6781 taaagaaata cagtttcctc attcccttgg gttgttgtac agcgcgatga ccacctatct
     6841 tggtttttct gccaatgacg cagagtataa ggtaatgggt ttagcagcat acggtaatgt
     6901 caaaccgttt ttaaaacact ttgatcaact aattacccaa tttgacgatg gtagctatca
     6961 gttaaacatg gaatatttca cttttgattg gtctaatcgc tcaatgtatt cttcaaaatt
     7021 aatcgagttg tttgggcatc ctgcgcgtaa gcccgagagc aaaatgatgt catatcacaa
     7081 gaacatagcg gcgtcattac aacaaaaatt agaagaagtt gtacttaaaa tgctaaacgt
     7141 tgcgtatgag acatataaaa ctccgaacct ttgtttagct ggcggagttg ctttgaattc
     7201 agtgctcaat ggaaaaatac taagtaaaac aaaatttaaa aacctttata ttccccctga
     7261 tcccagtgat gccggtggag caatgggagc cgctttttat gtatatcatc agaaaataca
     7321 caaagcaatt gatcacaaat catttaatcc gtatttaggg ccatcatatt cctggtttca
     7381 aattaaaaag gtattagatg ctcagcaaat taaatatacc ttttatgagt cgcgcgacga
     7441 tttaacaact aatgtggcgc agttggtaca gaaacaaaat attattggct ggtttcaagg
     7501 aagaatggag tggggaccgc gagcattggg caatcggagc atcttggcat ctgctgctac
     7561 caccaagatg agagatatta tcaatgaaaa ggtgaagcat cgcgaaatgt ttcgcccgtt
     7621 cgcgcccgtc attttagagg aacacattga agattacttt gttgccgata aaaatctccc
     7681 catttccgcc cgatacatgt tgatggtata tccttttaag ccaaaaggtg aaaaagacgt
     7741 tcctgcagtg gtacacgttg acggcagtgg cagacttcaa acattagtac gctccgataa
     7801 tccactgtac tatgaattga ttgaaaaata ttatcagcta accggagtgc caattataat
     7861 taatacctcg tttaacgtta gaggcgagcc aatagtctgt tctccagagg atgctgttaa
     7921 ttgtttttta aaaacagata tagattactt ggtcattgat cagtatgtat gccgaaaaaa
     7981 agactagcgt tggttttact cgtgttcagc gagattatag ctggattgtt ttttcttttt
     8041 tttgtccacc ttcactttac taataagaat gaaattgaac aggaaaaaaa agtagcctta
     8101 gtctcttctg agttcactca gcttcacaat agcgataggt ttcagtatta cacagagtta
     8161 agacctaatc tttctgagaa gaatcaacca tcctggcttt cccatgaggc agtatacacg
     8221 tataattcag atggactcaa cgatagatat gattactctt tagagaaaga taaaaatgtt
     8281 tttcgaatca ttgccttggg cgactctttt actttcggtc attatgttaa taccgaagat
     8341 tcttggccag agcaactaga agatatcctt aatcaggatg aactaaatca ttctgaaatt
     8401 tcatttgaag ttattaacct tggaatgaga ggttttgaca ttccatactc agttgagagg
     8461 tataaacgaa taggccagaa atactcgccc gacctgatta tttggtttga atctggatcg
     8521 ggctttcaaa gatatgtgga gttgttgtgg ccgataattt tctcctgcgt ggataataac
     8581 gaaaaaaatg cagcagatgc catatcattg gagaagttaa aaattgacta tcagcattta
     8641 gtaaagtgct ggattagtgg agaggagttt cttatccaaa aatattcgca gaaaacgatc
     8701 gaaacaaaac tggatgaatt tttagatgaa ttttacttac ttacagagaa tactccaaca
     8761 acatttatcc tatttgatag cagttcgcta aatcaagttc agaaagaaag ggtatcttat
     8821 ttgaaaaata gatacaaaag gttttcattt gagacaatcg ttcaagaatt aaagaccgaa
     8881 gaagtattac ccgatggtca cccttcaaga gagggtcacg aaaaaattgc ccagtctatt
     8941 caccactatt taatttcaag ctcgtcgttg ccaagctcaa cttgtgaagg agatcagtaa
     9001 tgaaggtagt tcttgcaact ctggtaatta ttgaaatatt agtgggagca agttttcttg
     9061 gttactacta taaagaaact agagtttcaa tgcgatcctt tgaccaaaga aaagttgcaa
     9121 taattaatcg cggtggtgtt gaaaaaaaac caagtcccaa cttcttgtat tacagtgagc
     9181 ttgtatcaaa caacattata gaaagtcaac tgccatggga tagtggggtt gaaattaaat
     9241 acaaaattaa taatgatggt ttaaatgata ggaaaaatta tgatttggtt aaacctaaaa
     9301 acacattccg tattatcaca ttgggagact cttttacctt tgggcactat gtgagtacag
     9361 ataataattg gccggaacaa ctagaggaat tattaaatac taaagaagtc tgcccatcca
     9421 ccactattga agtaattaat ttaggaatgt ggggttttga tattaactat ttgataaagc
     9481 gatataaaga cattggaaaa aaatatgaac cagacatggt tgtttggttt gagtcaggaa
     9541 gtggttttac aaggttggtt gaaacgatgg agccgctcag tgttgaatgt gaaaaacaaa
     9601 ttgagaattt tgtaagtttg gatgtattga aaagaattaa atattctggt gagtgttggg
     9661 gacaatctca acacaaggtt gttgttgacc taggagaaga tgggttgaaa agagttatga
     9721 ctacatattt ggatgattta tttactatac ttgatcaatc aattttcttc tatgttactt
     9781 ttgaaaaaat tgaaagcggg ataaacgcag aatcaactct gaatttttgg aaaaacagat
     9841 atgataaggt gcatttttcg aaaattccta acttaaaaca aggagagttg tttccagatg
     9901 gtcatccatc aactttggga cataagaaaa tcgcagagtc tatatttgag ctagtcaaag
     9961 aatccaactc tcaagaactg tgcattactg aaaattgata aactagtcta ctaggattgt
    10021 ttcttcaagt tattcaaaat aagtactgta tttttttgaa atctccttct gatagagtga
    10081 taaatgtttt ttaaatggac cactaaaatg gattgctgtc atgtttctat tcaaattccc
    10141 ttttttccat tctagctcag cataaacagt ataagagtct ggaaaagttt ttgcttttgt
    10201 tttttgtaat aaattagccc aaattgtttg atctaagtac cagtaagggt attcattcca
    10261 gcctccgatc accctttgtt tttgaacagt cttgattgtt ttaatgctct catgtatcga
    10321 ctcatttacc acaagatcat cttttggtcg atgaaataga actaagccaa catttaccgc
    10381 ttttgcaagt tctctattag taatcatttg agattctaag ttggagtaag agtaataatc
    10441 gcccacatct ttgaggtaat agttttgtgc tatttcaaac ttgatccact tatccacaag
    10501 ttg
//
LOCUS       DAOQZP010000035         9147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_8081_length_9147_cov_6.309393, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000035 DAOQZP010000000
VERSION     DAOQZP010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 9147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9147
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8081_length_9147_cov_6.309393"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..580
                     /locus_tag="PKH60_03370"
     CDS             95..580
                     /locus_tag="PKH60_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17114.1"
                     /translation="MKLLKTLLPNPLAFGIVGIYFFLSLILSFDKLLSLWFQIPILVV
                     GLVFGLILINIDRLYLHHLYRSSEDEGLVTQSLLFLGLFIPLSFFVVTSTGSILGGGI
                     IVGFLASTTASIYALRSDKQLLEKTYFYQLKRHLTEMEVQLVMAALLIWNSLVWLFAL
                     L"
     gene            593..1378
                     /gene="lgt"
                     /locus_tag="PKH60_03375"
     CDS             593..1378
                     /gene="lgt"
                     /locus_tag="PKH60_03375"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNQ17115.1"
                     /translation="MFGLSFHPYGLLIGIALVAGWECVQRACLRYGYSFKKMQKMYVL
                     IITIGFIFARLWHGITDWSLYVQNPAALLYVWNGGLSIIGGIVGGGLATMAILFTQKK
                     RTSLGRKPLTLFVVTDLLVHALPVSQAIGRVGNFVNQELYGLPTNLPWGIYIDPEHRL
                     PSVITQSYFHPLFLYEIILNLAIAVILWRRKWAIGAGGATGLYLMLYGIGRFLLDFLR
                     IDHGDLVWLGFSLNQYVLLMLAFGGLILLWRRRYHNTSTKTGK"
     gene            complement(1345..2169)
                     /locus_tag="PKH60_03380"
     CDS             complement(1345..2169)
                     /locus_tag="PKH60_03380"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNQ17116.1"
                     /translation="MVGFLVLVGFFLLQKKPLLVFVATIGFITQTIYLLPYYFQPKAS
                     ATIQAEHSTVRVYFNNINYGTTDFSSLVNSVTQYKPQIIGVAELSAEKFILLKNQLPS
                     FPYVYHVPGRGKLGLAIFSQLPFSDQPSTQYFSDHLFPSLVATVQDPDTFKQLKIIVI
                     HPPPPITGEYTSARNTLFDNLAEYSKSQTNPLLVMGDFNSTGWSPAFQQLLSNSGLID
                     SRNNQGTQPSWPSQLPKLLRIPIDHILTNEALLVSYREILPSVGSDHLPVLVDVLW"
     gene            2287..2835
                     /locus_tag="PKH60_03385"
     CDS             2287..2835
                     /locus_tag="PKH60_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNQ17117.1"
                     /translation="MTNVFHRKLGGNKKSVYHVVVKKLLFFLSLVVGVFFVTYLVSKS
                     AQLSNLQDGDTAQLLITREGTKKLIGSLFVSVVVSPSALERGLSGQSEVPMQGMLFVL
                     PERAIPNFWMKDMLFDLDLIWIDNSTIVDITSNVPAPDKQTPLSELARYSPTKPVTLV
                     LEIPAGQSDQLGLKIGDSIVIK"
     gene            2884..3771
                     /locus_tag="PKH60_03390"
     CDS             2884..3771
                     /locus_tag="PKH60_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17118.1"
                     /translation="MTTAPTGQAVFDPFAPLASTLPQSVAADPVVSSPVSATQAVNLP
                     DNSDNKNSSTQINVTPPVQVATTQVNPTVAPTENSDDASLHDTQDSDQINQEQEDVTV
                     DSPGQTVLQQVQINANQTLDVAGEQDFDLTEDEWLALLDQVLTETEQASSVFDQVMPA
                     AIIATDPLNPQGAVSSSARKPETVASNSAPQEAGLIGQVEYEPSSELPVEVEGFLERV
                     EDHADKLPQEIVIADEHAQLLPQSHPTQTVIMLPITEQDEENPVKDVKLSLTWLLEWS
                     HKIIKKFVGKVVYRHESNS"
     gene            3755..6097
                     /locus_tag="PKH60_03395"
     CDS             3755..6097
                     /locus_tag="PKH60_03395"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HNQ17119.1"
                     /translation="MNPTLNTTTLTSSVVTFFSQLALLLFVGTILAVLGYMIFQWFKH
                     RKREAYALNFVTLMIRLPKDNEIKIDAAEQMFSGLYSVKRRGILAWLKPEHSFAFEIV
                     ALKEEIAFYVSCPAALRDLVEKQIHGAYPNADIKEVEEVNIFSEKGKVDFAAICFEKS
                     NYFPIKTYKDLPTDGLSLITSALSKMGDGEGAIIQILLTPESKSWQQKGRGFVQSEKK
                     READPDKATYGHDPKEMEAINSKVEKPGFRVAIRVVVSSPNETTAESHVNNIVGAFSQ
                     FSSAYNSFKRGRVFLKHLFMIDFIYRYLPLFKRNTVILNTEEIATVFHLPNKTVETHH
                     IKWLNAKNAPAPNSIPNTGLYLGASHYRGDVRKVYIGHEDRRRHMYIVGKTGTGKSEF
                     LKEMILQDIEQGKGVCAIDPHGEFVEDILQLMPPERADDVIYFNPSDLDRPMGMNMME
                     ADTEEQRHFVVGSIIGLMYKLYDPHRTGIIGPRFEHAIRNAMLTIMYKPGSTFIELVR
                     ALTDQKYVQELLPIVKDPVVRRYWTDQIAQTSDFHKSEVLDYIVSKFGRFVTNKTMRN
                     IIGQPTSAFNFREAMDQNKIILCNLSKGILGEEDAKFLGLILVPKVLTAAMSRQDMAM
                     DKRNDFYLYVDEFQNYATEDFAVILSEARKYRLNLIVANQFVAQIDEDVKNAVFGNVG
                     TMVSFRVGVPDANFLQHEFAPTFNEIDLTNIEKYHVYMKTIVGNEPVPPFSMSLAKDM
                     DKVKARMNPKQAEMIKQLSRLKYGKDKDEVEREIQSRSNL"
     gene            6149..7846
                     /locus_tag="PKH60_03400"
     CDS             6149..7846
                     /locus_tag="PKH60_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNQ17120.1"
                     /translation="MSLDTAALQQILIQSGKIKSEDLTKWVDEAKSNKRDLASVVVEE
                     KLLTVEELGGILAKYFQVPFVNLLDKETDQAALEQIPPEVAKRRRVICYRQDPKEGLW
                     IATDDPQSNRLFVQLLSKKNGKPAQLAYTTSASIDQALTNYTVNFQKYFERFLRDDDS
                     FVIRTTVHDPPVKKLVDQLIEVAHEQNASDIHIEPREDDFLVRFRIDGMLHDIFTLPK
                     YLLDRVVTRIKVLSRLRTDQHLAAQDGKMTMMLNDDRLDLRISIIPVAEGEKVVARLL
                     TSKARSYSLEDLGFSEEALKRITNAYNQSYGMVLSTGPTGSGKTTTMYSILKLVNTRE
                     RNITSIEDPIEYRLPGANQVQVNTKTNLTFAAGLRSLLRQDPDVIFVGEIRDNETASI
                     AVNAALTGHLVLSTLHTNNAATTLPRLFDMEVEPFLVASTVRVIIGQRLVRKICAHCQ
                     TEEQIDTKELTKRLFIEKLPEKLEVKSSKIAIYKGKGCRFCHQTGYLGRIGIYEVLTV
                     SKVIRDLVSKRADSDVIHAQAVKEGMTTMFEDGLQKIIQGMTTVEEILRVTRTEEIK"
     gene            7846..8910
                     /locus_tag="PKH60_03405"
     CDS             7846..8910
                     /locus_tag="PKH60_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNQ17121.1"
                     /translation="MKTVNTNNLRLSGEDKISLFSNMATMLSAGIPIAETIESLLDES
                     KKDVRQLLLIVQEDISSGHHLHQSFSKFPKAFDKVTVNLIKSAEESGTLETSLKDIEK
                     NMLKEMQFADNVKSALMYPALVMVVFVVVLIVMLLVVIPKIAQVFSRLKVSLPFATRM
                     MIVLSNALIQHPFIVIGVSVVIVFGIMMFYRYKKELLFNFLYDLPGVSQLIRKTDLTH
                     FTRNLYLLLSSGIPIVMALELAEEVVRKPEVKALIKQARSRAVSGYPFFQGLRSKQRI
                     VPEMMIKLMEVGEKTGTLETAMQSVADHMDYEVTKSLAKFTTILEPVMLVVVALVVGG
                     MMMAIISPMYGLISQVGAMR"
     gene            8925..>9147
                     /locus_tag="PKH60_03410"
     CDS             8925..>9147
                     /locus_tag="PKH60_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ17122.1"
                     /translation="MKMTKIYAITGFTVLELMLTLSILGVLFALTVVNLTNLIPKANT
                     RAAAEVLVADLREQQMKAMSGYEAVSGGAT"
BASE COUNT         2773 a         1700 c         2028 g         2646 t
ORIGIN      
        1 gtcgccactt caatttatac aaactcattt tccggaaagt atgtgcctat aacagttcta
       61 cgaaaaatac tgtcgattag tgtacactag aatgatgaag ttactaaaaa ccctactgcc
      121 aaatcccctt gcttttggaa ttgttggaat ctattttttt ttgtcactga ttctctcgtt
      181 tgataaatta ctttctctct ggtttcaaat tcccatttta gttgttggtt tagtgtttgg
      241 tctgattttg ataaacatcg atcgcttata tctccaccac ttgtatcgat caagcgaaga
      301 cgaaggttta gtgacacaat ccttgttatt tcttggctta tttattccgc tctctttttt
      361 tgttgtgact tctactggca gtattctagg cggtggaatt attgtgggat ttttggcaag
      421 cacgacggcc tcgatctatg ccttgagatc agacaaacaa ttactagaaa aaacatattt
      481 ttaccagctt aagcgccacc ttaccgagat ggaggttcaa ttggttatgg cagcgctatt
      541 gatttggaat agtttggtct ggttatttgc gctcttataa tacaatggtt gtatgtttgg
      601 actgtcgttt cacccctatg ggttacttat tggtatcgca cttgtggctg gttgggaatg
      661 tgtgcaacga gcgtgtttga gatacgggta ttcattcaag aaaatgcaaa aaatgtatgt
      721 gctcattatc acgattggat ttatttttgc tcgattgtgg catgggataa ctgactggag
      781 tttgtatgtc caaaaccctg ctgcattgct ctatgtttgg aatggtggcc tcagtattat
      841 tggagggatc gttggtggtg ggttagctac aatggccata cttttcacac aaaagaagcg
      901 aacaagtttg ggacgaaaac cacttacatt gtttgtagta actgacttac tcgtacatgc
      961 tttgccagtg agtcaggcaa ttggccgggt tggaaatttt gtaaaccaag aactgtatgg
     1021 tttgccaacc aatctgcctt ggggaatata tatcgatcca gaacatcgat taccgtcagt
     1081 tatcacacaa agctattttc atccgttatt tttgtatgaa ataattttaa atctagccat
     1141 cgcagttata ctgtggcgga ggaagtgggc tattggagct ggtggagcaa ctggattgta
     1201 cttaatgctc tacggtattg gccgcttttt acttgatttc cttagaattg accatggcga
     1261 tttggtttgg cttggtttta gcctcaacca gtatgtgttg ctgatgctgg cttttggtgg
     1321 tttgatactg ctctggagaa ggcgttacca taacacatct accaaaacgg gcaagtgatc
     1381 agagcccacg gatggtaaga tttctcgata ggaaacaaga agtgcttcat tggttagaat
     1441 atgatcgatg ggaattcgca gtaactttgg cagctgactt ggccaggatg gttgagttcc
     1501 ttgattatta cgtgaatcaa ttaagccaga atttgataat aattgttgaa atgcaggcga
     1561 ccacccagtg ctattaaaat cacccataac caagagaggg tttgtttgcg actttgaata
     1621 ctcagcaagg ttatcaaata acgtatttct tgcactcgtg tattcgccag taattggagg
     1681 tggaggatgt ataacaatta tctttagttg tttaaatgta tctgggtctt gaacagttgc
     1741 gactagtgaa ggaaatagat gatctgagaa atattgagtg cttggctgat cagaaaaagg
     1801 taattgagaa aatattgcca gtccaagttt gcctctacca ggtacgtgat aaacatatgg
     1861 aaagcttggc agttgatttt ttagcaaaat gaacttttct gcagataact cagcaacacc
     1921 gataatttga ggtttgtatt gtgtgacaga gttgactagt gaggaaaaat cagtcgttcc
     1981 ataattgata ttgttaaaat atactcggac ggtagagtgc tcagcttgga tggtagcaga
     2041 agctttgggt tgaaaataat atggaagtaa atagatagtt tgagtgatga aaccgattgt
     2101 ggcaacgaaa actaagaggg gttttttctg caacagaaaa aacccaacta gtacaagaaa
     2161 tcccaccatg tattgaaaat gaaaatgcga tagcagatca agtagaaagt gtgtggatcc
     2221 aaaaaaactt aaaatagaag ttagcaacag tacagccaaa gcaagatagt tcaaggtaga
     2281 tacaacatga ccaacgtttt tcatagaaaa ttaggcggca acaaaaaatc agtatatcat
     2341 gtagtagtga aaaagctgct tttctttctc tctttagtag tcggtgtatt ttttgttact
     2401 tacttagttt ccaagtctgc acagctatca aatttacaag atggtgatac tgcgcaatta
     2461 cttattacta gagaggggac aaagaaacta attggatcac tctttgtcag tgtggttgtt
     2521 tcaccaagtg ctctagagcg tggcttgagt ggacaatctg aggtacccat gcaaggaatg
     2581 ctatttgttt tgccagaacg agccattcca aatttttgga tgaaagatat gctatttgat
     2641 ctggatttga tttggatcga caattccaca attgtagata taacttcaaa tgtcccggca
     2701 cctgacaaac agacaccgct cagtgagctt gctcgataca gtccaacaaa gcctgtgacg
     2761 ttggtcttgg agattcccgc gggtcaaagc gaccaactgg gattgaaaat tggagatagt
     2821 attgtcatta agtgacagtt ccttctgctt ctcttcatac cctcaagatg ctatgattga
     2881 attatgacaa ctgccccgac aggacaggcg gtatttgatc cgtttgctcc actagcaagc
     2941 acgttgccac agtcggtggc agctgatccg gtggtttcct ctccagtgag tgccactcaa
     3001 gcagtcaatc tgccagataa ttcggacaac aaaaattcgt caactcaaat caatgtgact
     3061 cctccagttc aggttgcgac cacccaagtt aatccaacgg ttgcaccgac cgagaactca
     3121 gatgatgctt cgctccatga tacccaagat tcagatcaaa ttaatcaaga acaagaagat
     3181 gtgacagtgg attcaccagg tcaaactgtt ttacagcagg ttcaaatcaa tgcaaatcaa
     3241 acactagacg ttgccggaga acaagacttt gatttgactg aagatgagtg gctggcactg
     3301 cttgaccagg ttctcacaga aaccgaacaa gcttcaagtg tatttgacca ggtaatgcca
     3361 gccgctatta ttgccactga tccactcaat cctcagggag cagtttcctc aagtgctcga
     3421 aaaccagaga cagttgcttc aaatagcgct ccacaagaag cagggctgat tggacaggta
     3481 gaatatgagc cgtcatccga attaccagtt gaagtagaag gatttttgga gagagtagaa
     3541 gatcacgcgg ataaattacc acaagaaatt gtaattgcag atgaacatgc gcaactcctg
     3601 cctcagtcac atcccacgca aacagtaatc atgctgccaa taactgagca agatgaagaa
     3661 aatccggtta aagatgtaaa actgagtctc acttggttgc tggaatggag tcacaagatc
     3721 attaaaaaat ttgtaggaaa agtagtgtac cgacatgaat ccaactctta atacaaccac
     3781 gctcacaagt tctgtggtga ctttttttag tcaacttgct ctactactat tcgttggcac
     3841 gattctagcc gtattaggct atatgatttt ccaatggttc aagcatcgga agagagaagc
     3901 gtatgcctta aacttcgtga cgttaatgat tcggttaccc aaagataatg aaataaaaat
     3961 agatgccgca gagcaaatgt tttctggact ttattccgtt aagcgaaggg gcattcttgc
     4021 ttggctaaaa ccagaacatt cttttgcctt tgaaatagtt gccttaaaag aagaaattgc
     4081 tttttatgtg agttgtccag cagcactgag agatttggtt gaaaagcaaa tacatggtgc
     4141 ctatcccaat gcagacatca aagaagttga ggaagtgaat attttcagtg aaaaaggaaa
     4201 agtagatttt gccgcgattt gttttgaaaa atccaattat tttcctatca aaacatataa
     4261 agacttgcct actgacggat tgtcacttat cacgtcggcg ctttcaaaga tgggagatgg
     4321 tgagggagca attattcaaa ttttattgac gcctgaaagt aaaagctggc aacaaaaagg
     4381 caggggtttt gttcaatcag aaaagaagcg cgaggcagat cctgacaaag caacgtatgg
     4441 gcacgatccc aaggaaatgg aagcgattaa ttctaaggtt gagaagccag gattcagagt
     4501 ggctattcga gtggtggtca gttcacccaa cgaaaccaca gccgaatcac acgtcaataa
     4561 tattgtgggc gcattctctc agttttcttc tgcatacaat agtttcaagc gaggtagggt
     4621 gtttttgaaa cacctattta tgatcgattt tatttatcga tatttaccgt tattcaaacg
     4681 taacacggtg attctcaata cagaagaaat tgccacagtg tttcaccttc caaataaaac
     4741 ggtagaaacg caccatatta aatggctcaa tgccaagaat gcgcccgccc caaattcaat
     4801 tcccaacact ggattatatt tgggcgcctc acactaccga ggagacgttc ggaaagtcta
     4861 tataggtcac gaagatcgtc gacgccacat gtatatcgtg ggaaaaactg gtaccggtaa
     4921 atctgaattc ttgaaagaaa tgatcctgca ggatattgag cagggcaaag gtgtttgtgc
     4981 gatagaccca cacggtgaat ttgtggaaga tattttgcaa ctgatgccac cagaaagagc
     5041 tgatgacgtg atttacttta acccatcaga tttggacaga ccgatgggta tgaatatgat
     5101 ggaggcagac actgaggaac aacgacattt tgtcgtaggt tccatcatcg gcttgatgta
     5161 caaattgtac gatccacatc gaacgggaat tattggtccg cgttttgagc atgctattcg
     5221 taacgcaatg ttaacgatta tgtataagcc aggatcaaca tttattgaat tagttcgagc
     5281 gctcacagat caaaaatatg ttcaagaatt gttaccaata gtgaaagacc cagtagttcg
     5341 tcggtactgg acagatcaga ttgcccagac ctctgatttt cacaaatcgg aagtacttga
     5401 ctatatcgtt tctaaatttg gtcggttcgt tacgaataaa acgatgcgca atattattgg
     5461 tcagccaaca agtgcgttta attttcgcga agcgatggac caaaataaaa ttattctttg
     5521 caatctgtcc aaaggtatct tgggtgaaga agatgcaaaa tttttgggtc taatcttggt
     5581 accaaaagta ctcaccgcag ccatgagtcg gcaagatatg gcaatggata aacgcaatga
     5641 cttttatcta tacgtagatg agtttcaaaa ttatgctaca gaagattttg cagtcatttt
     5701 gtccgaagct cggaaatacc gactcaattt gattgtggcc aatcagtttg tggcgcaaat
     5761 tgatgaggat gtaaaaaatg cagtttttgg aaacgttggt accatggttt ctttcagagt
     5821 cggtgtgcca gatgcaaact ttttacagca tgaatttgcg cctacattta atgaaatcga
     5881 tttgacgaat attgaaaaat atcatgtgta tatgaaaacc atagttggta acgagccagt
     5941 gccaccattt agtatgtctt tggccaaaga tatggataag gtgaaggcgc ggatgaatcc
     6001 caagcaggcc gaaatgatta aacaactgtc tcggctgaaa tacggaaaag ataaagacga
     6061 ggtagaacga gagattcaat cgaggagtaa cttgtaatat tagccagttg tgaccgcgtg
     6121 tcacactggc tatgataata acacacacat gagtttagac actgccgccc tacaacaaat
     6181 tctgattcaa tcgggaaaga ttaagtctga ggatcttacg aaatgggtag atgaagccaa
     6241 aagtaataag cgagatctgg ctagtgttgt agtggaagaa aaattactta ctgtagaaga
     6301 acttggtggt attcttgcga agtattttca agttcctttt gttaatctat tagacaaaga
     6361 aactgatcaa gctgcactag agcaaattcc cccggaagta gccaagcgac ggcgagtaat
     6421 ttgttatcgc caggatccaa aagaaggttt gtggatagcc actgatgacc cgcaatctaa
     6481 tcgattgttt gttcaactgc tttctaagaa aaatggtaag ccagcacagt tagcgtatac
     6541 cacttcggct tcaattgatc aggcgctcac aaactacacg gtaaactttc aaaaatattt
     6601 tgaacgattt ttgcgtgatg atgatagctt tgtgattcgc acgacggtac atgatcctcc
     6661 cgtcaaaaaa ttagttgacc agttaataga agtggctcac gaacagaatg cttcggatat
     6721 tcacatcgaa ccaagagaag atgattttct tgttcggttt cgaatcgacg gcatgttaca
     6781 cgatatattc actttgccaa aatatttgtt agatcgggta gtaactcgca tcaaagtgct
     6841 ttcaagattg cgtaccgacc agcacttggc agcccaagac ggcaaaatga ctatgatgct
     6901 caatgatgac cgccttgatt tgcgtatctc aatcattcct gtagcagaag gtgaaaaagt
     6961 cgtggctcgt ttgcttacct caaaagctcg ctcatattca cttgaggatt tgggctttag
     7021 tgaagaagcg cttaaaagaa ttactaatgc atacaatcaa tcgtacggga tggtgttgag
     7081 tactggtcca accggatccg gtaaaacaac taccatgtat tcaattctca agttagttaa
     7141 cacgagagag cggaatatta ctagtataga agatccaatt gagtatcgtt tgccaggggc
     7201 caatcaagta caggttaaca ccaaaaccaa cttgacgttt gccgcaggac ttcgttcttt
     7261 attgagacag gatccggacg taatctttgt gggagaaatc cgcgataacg aaactgcctc
     7321 gattgctgta aacgccgcgc tcactggcca tctagtgctc tcaacgctgc atacaaataa
     7381 cgctgcaact actttgccaa gactatttga tatggaggtt gagccatttt tggttgcttc
     7441 aacagtgaga gtgatcattg gtcaaagact ggtgcgtaag atttgtgccc actgccaaac
     7501 ggaagaacaa attgatacaa aggaattaac caagcgttta ttcatcgaga aattaccaga
     7561 aaaattggaa gttaagagta gcaaaatcgc catttacaaa ggaaaaggtt gtcgattttg
     7621 ccaccaaacc ggttacttgg gaagaattgg tatctatgaa gtcttgacag tttccaaagt
     7681 aatccgcgat ttggtttcca agcgagcaga ttctgacgtg attcatgccc aagccgttaa
     7741 ggaaggtatg acaaccatgt ttgaggatgg tctgcaaaaa attattcaag ggatgaccac
     7801 tgttgaagaa attttacgtg taactcgcac ggaagaaatc aaataatgaa aacagttaac
     7861 actaataatt tacgcttgtc tggtgaagac aagatctcgc tttttagcaa tatggcaacc
     7921 atgttgtccg ctggtattcc aattgcagaa acgattgagt cgctattgga tgaatcaaaa
     7981 aaagatgtac gacagctact cttgatagtt caggaagata ttagttctgg tcaccactta
     8041 catcagtcat tttctaaatt tcccaaggcg tttgacaagg tgacagtaaa tctaattaaa
     8101 tctgccgagg aatctggtac gttggaaacc tcactcaaag atatcgaaaa aaatatgctg
     8161 aaagaaatgc agtttgcaga caatgtgaag tccgcactca tgtatccagc gctcgtcatg
     8221 gttgtatttg tggttgtttt aatcgtaatg cttttagtag tgattcctaa gatcgcccaa
     8281 gtatttagtc gtttgaaagt gtccttgccc tttgctactc gaatgatgat tgtcctgtcc
     8341 aacgctttga tccaacatcc atttatcgtc attggtgtat cggtagtaat tgtttttgga
     8401 attatgatgt tttatcgtta caaaaaagaa ctattattta attttttgta cgatttgcct
     8461 ggagtttcac aactaattcg taaaacagac ttaactcact ttacgagaaa cttatattta
     8521 ctattgtcat ctggaattcc aattgtaatg gcgttggaat tggccgagga agtagtgaga
     8581 aaacctgagg taaaagctct gattaaacaa gctcgaagtc gggcggtttc cggatatccg
     8641 ttttttcaag gtttgcgcag caagcaacgc attgtgccag aaatgatgat caaactaatg
     8701 gaagtgggtg agaagactgg aaccttagaa actgctatgc aatcagtcgc cgaccatatg
     8761 gattatgaag tgacaaaaag tttggccaag tttactacta tcttggagcc ggtcatgcta
     8821 gtagtagttg cactcgtagt tggtggcatg atgatggcga ttatttctcc aatgtatgga
     8881 cttataagtc aagtgggagc catgcgctaa ttatgtgaag ttctatgaag atgactaaaa
     8941 tatacgcaat tactggtttt acagtattgg aattaatgct cacactctcg attcttggag
     9001 tgttatttgc attgacagta gttaatttaa ccaaccttat tccaaaggca aatactagag
     9061 cggcagccga agtattggtt gcggatcttc gtgaacaaca aatgaaagca atgtctggtt
     9121 atgaggcagt cagtggtggt gccactg
//
LOCUS       DAOQZP010000036         9017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_8211_length_9017_cov_5.517072, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000036 DAOQZP010000000
VERSION     DAOQZP010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9017
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8211_length_9017_cov_5.517072"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1981
                     /locus_tag="PKH60_03415"
     CDS             <1..1981
                     /locus_tag="PKH60_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17123.1"
                     /translation="VATSIYTNSFSGKYVPITYSITSMTEWYDYVIMLKKLIWLPIIS
                     FLIFSRLFLLNSVPGTIPHDEMVYAIQAKSYATQGTTLDQIHRPWMISPFDDMYAELP
                     ATFMALGYLLSKDPLVGAHLTSALMGISMPFILAWLFFGLWKRKDLAIGVAIVSVFNP
                     LFWQMSRLGYDNWYSLWFYLLGAALLVQKNKSLQWLSILVFGIGFFNYQGYKLLLFPW
                     AIIVVLLKITAGKSLPTWSDFEQFVTKRKLMITSVKKGFFLYLPQCMAVIFSLILTSF
                     YGLVLLPKQVGVSERLGMTIFSDTEKIAQLVDLERRQTFVSPLLNVYTNKATITLNFM
                     LDRLANAFDLNLLFLQVEPAVSGFSVWVHGIFYWIEGIFFLIGIVWLLSQPKHRANAL
                     ILLIGILGLCLPTLINTGSEWHLLRTMFSYTLLIVFAGIGLSWILRHRFVLAILLPFY
                     LLAIGNFTYIYFFRYPVISLDWGNYHERLVARYITLLSLEHPDTQVSVHVGQPNYYYW
                     SYLLYSDRLTDENKELVAQNALLDTKAPVQSFKIDNVTFTSSCEVQSNTGVMVAETSF
                     QDCETEVANFDRAKIGQNQIASDSASIQKQRSFNSIVAILDSGEKLYILDDQLCTGKK
                     LKSYVFPTSYKDIGVEKLDTDQFCLNWISDQSIVK"
     gene            2008..2090
                     /locus_tag="PKH60_03420"
     tRNA            2008..2090
                     /locus_tag="PKH60_03420"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2042..2044,aa:Ser,seq:gga)
     gene            complement(2213..2902)
                     /locus_tag="PKH60_03425"
     CDS             complement(2213..2902)
                     /locus_tag="PKH60_03425"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNQ17124.1"
                     /translation="MQFLISWLEALSQQIPVTWFVFIGALVEEVIAPIPSPLVMMLAG
                     SISASQSISLLFILVLALIGALSKTIGSFIIYVIADKAEDIIISKFGKFLGVSRSDTE
                     GLGKFLGEGKREGFAIFLMRAIPVMPTAPVSVIAGLIKVNFRSYLFSTFLGLIVRNSI
                     YLYLGYTSLGALESLNEGFDSLENIGYLVLAVFAGMALLWMYRKRQQGSVLDLVEKML
                     SFTKKVFSKRK"
     gene            complement(2985..4886)
                     /locus_tag="PKH60_03430"
     CDS             complement(2985..4886)
                     /locus_tag="PKH60_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17125.1"
                     /translation="MRNLEYTPIQTEDVFIQWLTHQEHSGESITSAQLAEAQFYLLQQ
                     YDHGHEIKLDHLKRLEKLYPQGLLNGRPDDPNTVASMIKYVGERMPTLIFRKKLKAIH
                     FTNSTSEIMDVVHVSRAPGRVKRRKHSPKRCRWCTRFDFDEQHSEVTFHVRDVLLKDP
                     TDTFEMLSWFGSLEQARESVVAMIKRGEDITPIKIAFRELYETHVRSEIEMLKKKKVA
                     LKNKDPELLQSINYRLEELVDRMNHLDSLAEELRTEWVSKDREDAIICPEHPNQKVLG
                     GWSGIKEPNAAEMTMDIAKNKIETEKLKNPTDLLFGNDFSMRIFLSIGLFSEQEFVQM
                     GKAFEAKRSEISSSLEKLSSQEVRDAAEKFFLVQQLSQYVGSDLVIQKLRERLFSSET
                     FEKIGINRLVATVDSPIADPVDLIDVTQIHSEIFSNLPTFLQTILTKLKTNNRTILSV
                     PYTLGNLSRTTLEAAVAEFPSLESENFIGKVGNYSKGVNQNISVGDVILGKQVFSQFE
                     DDSKPIQNPISELIAQYGYPVKFASLLTTAGLTFQQPAEMAEILDDPLIEELCLEMEA
                     FHLMEALENLTLRSNHLYYVSDKTIDPSHYNENLHAGDKISESLGPRGSVPLFICLLA
                     FLENLSKPK"
     gene            4945..5517
                     /locus_tag="PKH60_03435"
     CDS             4945..5517
                     /locus_tag="PKH60_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNQ17126.1"
                     /translation="MHFPLFHKRDTIVYADDDDVSIFNLIRLRFPPLGLGLLLGILLS
                     IVASRFEEVLAIDVRVAFFIPFIVYMADAVGTQTQTIYSRDLRSGKASFKKYLYKESV
                     LGLLLGLLSGCMSAIVTMLWFNSTQLATSVSIAMFASIAFAPVVALLVTEVLQLEHKD
                     PAVGAGPIATVIQDTLSVLIYGLVASFVFF"
     gene            complement(5557..6018)
                     /locus_tag="PKH60_03440"
     CDS             complement(5557..6018)
                     /locus_tag="PKH60_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNQ17127.1"
                     /translation="MIPKTIPFTKAKYDEMQRRYDFLQKELVLVMARLKVAREMGDLS
                     ENGAYKYAKFEIGDIRRQSSKLKHLLIHGYVETNTKHLGKIGFGSVVTVKSKKGEYTF
                     TLVSQYESDPQNNKLSEESPLGKAVYGKQSGENVAVISPSGEMEYSIEKVQ"
     gene            6089..7159
                     /locus_tag="PKH60_03445"
     CDS             6089..7159
                     /locus_tag="PKH60_03445"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNQ17128.1"
                     /translation="MKKHSVSIDLIRVIAILGALFIHLTYPIYARPDFFGGTTWWLTT
                     IISAFSRISIPLFIILSGYLLLPKNESWATTQARLVHRLLVPFVFWFGFYLIWDRIFL
                     HRTNSLLEIPTLLYQSNTYHLYFLLILTGLYFLLPMLRLLFVHLENHYIKVLLGVGFI
                     AGVLMYFVPYGLIRDQAQFNIFTAWVPYLGYFLFGGFLATKKPQKPRWLGLVTLFAFI
                     TTIALSASVVAFRETTTAWLWRSHGVAFVDEYLSPQIIVFALSFFWLLVSLPPNFKLL
                     NNLHVSKLLRQLAKASFGAYLIHVLVLNVLDFRFGLALDAYPGSLLQYFLIRSSLCLL
                     LSFGLSWLLLQIPWVKKVFGEN"
     gene            7213..7845
                     /locus_tag="PKH60_03450"
     CDS             7213..7845
                     /locus_tag="PKH60_03450"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ17129.1"
                     /translation="MKFTEAARLTAQAYFWYHSKNSDTQYTNGPMHLYDAAAESLTDR
                     NAVGKLVAQLVGNPTGRILEIAAGTGLISQHLADVSQLTCSDISQESLELLRERVTSA
                     NILSADFTNLPFSDQTFDTVVNVGGFRYVTDEVAFWNEMDRVTSKTGRVIIVQFYPRL
                     SKLSGYDLRVDSQHQISFKKNKILTHKSNVTIGSMSVTTGEYLLIEFTKK"
     gene            7869..8867
                     /locus_tag="PKH60_03455"
     CDS             7869..8867
                     /locus_tag="PKH60_03455"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HNQ17130.1"
                     /translation="MSLKFQLLPTRVYFEDLLLEINHAKKHICIQSLYFDPGHYLDKI
                     ITALIKKQKEGLFVELIIDAFGKSPQIKTHRFESALAKLIDNHVAVTFINPLPSRQNW
                     IFPYLHRNHIKIVLIDNTIYFGGINFSNQELTFQDLMIKVVDEKISTIFEQIINESKQ
                     QILEDSSIRLDNKNILMVEGINTQSIIYQRALSLINDAKKSIVFTSQFSPDGKMLDSL
                     YQSTSRGVKLTIIVPVRNHFNRLFELLNQWNNLMIIIKQKNLPLLKIKSMIHAKLLLI
                     DNQTLLIGSHNLCWLGVASKSSEIMLQSTDSNLINQVKQFTNHLITDNQGDSCCNH"
BASE COUNT         2608 a         1883 c         1614 g         2912 t
ORIGIN      
        1 agtcgccact tcaatttata caaactcatt ttccggaaag tatgtgccta taacatactc
       61 tataacgagt atgacagaat ggtatgatta tgtcatcatg ctaaaaaaat taatctggtt
      121 gccaattatt agttttttaa ttttttctcg gctgtttctc ttaaattctg taccgggcac
      181 tattcctcat gatgaaatgg tctacgccat ccaagccaaa tcatatgcaa ctcaaggcac
      241 tacactcgat caaattcatc gaccctggat gatctctcca tttgacgaca tgtacgctga
      301 attaccagct acttttatgg ccttaggata tttgctcagt aaagacccgc tcgtcggcgc
      361 acatctaacc tcagctttaa tgggcatcag catgccattt attcttgctt ggctcttctt
      421 tggattgtgg aaacgcaaag atctagctat tggcgttgca atagtatctg tttttaaccc
      481 tctcttttgg caaatgagcc gcctcggtta tgacaactgg tactctctct ggttttattt
      541 gcttggtgca gctctactag tgcaaaaaaa taaatcactt cagtggctat caattttggt
      601 ttttggcatt ggctttttta attatcaggg atataaatta ctgctttttc cttgggcaat
      661 cattgtagtg ttgttaaaaa ttactgcagg aaaatcactg ccaacttggt ccgactttga
      721 gcagtttgtt accaaacgaa aattaatgat tacttcagtc aaaaaggggt ttttcctcta
      781 cttgccgcaa tgtatggcag tgatattttc gttgattctc acttcatttt atggcttagt
      841 tttactgcca aaacaagttg gtgtgagcga gcgattggga atgacgattt tttctgacac
      901 agaaaaaatc gcccaattag tcgacctgga acgcagacaa acctttgttt cacccctact
      961 caacgtatac accaacaaag ccactatcac tttgaatttt atgcttgatc gactggccaa
     1021 tgcttttgat ctcaacttgt tgttcctcca agttgaaccg gccgtaagtg gttttagtgt
     1081 ttgggttcat gggatttttt actggattga aggcatattt tttctcatcg gaatagtgtg
     1141 gctgctaagt caacccaaac accgcgccaa tgctctgatt ttattaatcg gaattttagg
     1201 attgtgtctc cccactctca ttaacacagg cagtgaatgg catttattgc gaactatgtt
     1261 tagttatact ctactaatag tgttcgctgg tattggactg tcttggatac tcagacatcg
     1321 tttcgttttg gcaatactct taccattcta tctattggcg atcggcaact ttacgtacat
     1381 ctactttttt cgctatcccg tgattagttt ggactggggt aattatcatg aacgactagt
     1441 ggcacgctac attactctac tatcgttaga acatccagat acccaagtaa gcgtgcatgt
     1501 aggccaaccc aattattact actggagtta cttgctgtac agcgatcgtt taacagatga
     1561 aaacaaggag ttagtagcac aaaatgcatt acttgatacc aaagcgccag ttcaatcatt
     1621 caagattgac aatgtgactt tcactagtag ctgtgaggta caatcaaaca cgggggtaat
     1681 ggttgccgaa accagctttc aagattgtga aacagaagtg gctaattttg atcgagccaa
     1741 aattggtcag aatcaaattg cttccgattc cgcttcgatt caaaaacaaa gatcatttaa
     1801 ttcgattgtg gcaattttgg atagcggcga aaaactgtac attttggatg atcaattatg
     1861 tacaggtaaa aaactgaaaa gctatgtgtt tcccacttcc tacaaagata taggtgtaga
     1921 aaaactggat actgaccagt tttgcctaaa ctggataagt gatcaatcaa ttgtaaaata
     1981 gcgggtataa tagcaatcta acaaagtgga agcgtcgcat agcggtcaat tgcacacgct
     2041 tggaaagcgt gagccttacg gcttcgtggg ttcgaatccc accgcttccg cgagaatccg
     2101 cctatggcgg agacgagcga gacaggtgga atgagaagtc aaccgagttg tgacaaacaa
     2161 cccggttatc ccgatgaaat cgggaaatcc caccgcttcc gccattttga gtctattttc
     2221 tcttgctgaa taccttcttt gtgaagctca gcatcttttc taccaaatct aagacggaac
     2281 cctgctgacg cttccgatac atccaaagca atgccatacc tgcaaaaaca gctaaaacta
     2341 agtacccaat attttctaaa ctatcgaatc cctcattgag tgattctaga gctcccagac
     2401 tggtataacc caaatagaga tatattgaat ttcgtacgat taaacctaaa aatgtgctaa
     2461 ataaatacga acgaaagtta acttttatca agcccgcaat gacagatact ggggctgttg
     2521 gcataacagg tatggctctc attaagaaaa tcgcaaaccc ctctcgctta ccttcaccca
     2581 aaaatttgcc caagccctct gtatcggaac gagatactcc tagaaatttt ccaaatttac
     2641 taataataat atcttcagct ttatcggcaa tgacgtagat tatgaagcta ccaattgtct
     2701 tactgagtgc tccaatcaac gcaagtacca aaatgaatag tagtgaaata ctttgacttg
     2761 cagaaattga acccgccaac atcatcacta agggcgaagg aataggtgcg atcacttctt
     2821 ctaccaaagc accaataaaa acaaaccaag taacgggaat ttgttgagaa agtgcttcta
     2881 accaagaaat caagaattgc atagaaccta gattctacta tagatacgaa cgaaatccta
     2941 tgtcacaacg aatgagcgtg attctttggc tcgtgatgaa acaactattt tggtttactg
     3001 agattctcta aaaatgctaa taaacaaata aatagaggga cgctacccct tggtcccaat
     3061 gactctgaga tcttatctcc agcgtgtagg ttttcattat aatgcgaggg atcaatagtt
     3121 ttatctgaaa catagtacaa atgatttgat ctgagcgtta aattttctag cgcctccatt
     3181 agatgaaatg cttccatttc cagacataat tcctcaatta gtggatcatc taaaatttct
     3241 gccatttctg ctggttgttg gaaggtcaaa cccgccgttg ttaataaact ggcaaatttc
     3301 actggatacc catattgagc gattaattct gagattggat tctgaattgg cttactatca
     3361 tcttcaaatt gtgaaaatac ttgctttccc aaaatcacat cacccaccga tatattctga
     3421 ttaactcctt ttgaataatt tccaactttg ccaataaaat tctcactttc cagagaggga
     3481 aattcagcaa ctgcggcttc caacgtggtt cttgatagat ttcccaatgt atacggaacc
     3541 gatagaatag tccgattatt agtttttagt ttcgttagta ttgtctgcaa gaatgttggc
     3601 aaattagaaa atatttccga atgaatctgc gtgacatcta ttaagtcaac tggatctgct
     3661 attgggctgt caaccgtcgc taccaatcta ttaataccaa tcttctcaaa tgtttctgag
     3721 ctaaacagtc gctctcgtaa tttttgaata actaagtcac tcccaacata ctgtgatagt
     3781 tgctgaacta aaaagaattt ttctgcagca tccctaactt cctgactgga aagtttttct
     3841 agtgaagatg aaatttcact tctttttgct tcaaaagctt ttcccatttg tacaaattct
     3901 tgttcagaaa ataatccaat ggataagaag atcctcattg aaaaatcatt gccaaataaa
     3961 agatctgttg gatttttcag cttttctgtt tcaattttat ttttagcaat atccatcgtc
     4021 atttctgcag catttggctc tttaatccca ctccatcctc caagtacttt ttgatttgga
     4081 tgctctggac aaattatcgc gtcctctcga tccttactta cccattccgt acgtagttct
     4141 tcggccaaag agtcaagatg gttcattcta tctactaatt cttccaggcg ataattgatc
     4201 gattgcagta attcaggatc tttatttttc aacgccactt tcttcttctt caacatctca
     4261 atttctgatc tgacgtgggt ttcatataac tctctaaagg caatcttaat gggtgttata
     4321 tcttcgcctc gtttgatcat ggccactacc gattctctgg cttgctctaa acttccaaac
     4381 caagacaaca tttcaaatgt atcagttgga tcttttagca atacatctct tacatgaaaa
     4441 gttacttctg agtgctgctc gtcaaaatca aaacgagtgc accaacgaca ccgtttggga
     4501 ctatgtttcc ttcgcttaac tcttcccgga gctctagaca catgtacaac atccataatt
     4561 tcactggtac tattggtaaa gtgaatcgcc ttcaattttt ttctaaaaat caaagttggc
     4621 attctttcac caacatattt gatcatcgaa gcaacagtat ttggatcatc aggccgacca
     4681 ttaagcaagc cttggggata cagcttttcc aatcgcttga gatggtcaag cttgatttca
     4741 tgaccgtgat catattgctg taataaataa aattgagctt ctgctaattg ggcagaggtt
     4801 atgctctcac cagaatgctc ttgatgagtc agccattgga taaatacgtc ctccgtctga
     4861 ataggagtgt actccaaatt cctcatagta aacctatatt atacatattt gatgtgtaaa
     4921 aatcaaatgg tatacaataa aagtatgcat tttccacttt ttcacaagcg agatactatt
     4981 gtttatgccg atgatgacga tgtatcaatt tttaatctaa ttcgactacg attcccccca
     5041 ttagggctcg gattattgct aggaattttg ctatccattg tggcatcacg ctttgaagaa
     5101 gtacttgcca tcgatgtgag agtggcattt tttatcccat ttattgtgta catggctgat
     5161 gcagttggca ctcagactca aacaatttat tctcgagatt tacgttcggg taaagccagt
     5221 tttaaaaaat atttatataa agaatcagtg ttggggttac tgcttggatt actctctggc
     5281 tgtatgagcg caattgtcac aatgctctgg tttaattcta ctcaactagc cacttcagtt
     5341 tctattgcga tgttcgcatc aattgcgttt gctccagttg tcgcgctgct cgttaccgaa
     5401 gtcttgcaac ttgagcataa agatcccgcc gtgggggcgg gtccaattgc cacagtcatc
     5461 caggatacat tgagcgtgct gatttatggc ttggttgcca gctttgtgtt tttctaaaca
     5521 gacgctgatg agcgtcgatg gaagtactat cccatcttat tgaacttttt caatcgagta
     5581 ttccatttct cccgagggag atattacagc aacgttttct ccactctgtt taccatacac
     5641 tgctttaccc aagggacttt cttctgaaag cttgttgttc tgcggatcag attcatattg
     5701 actgactaaa gtaaaggtat attcgccttt tttgctctta acggttacca cgctaccaaa
     5761 accaatttta cccaagtgtt ttgtgttcgt ttctacgtag ccatgaatta atagatgctt
     5821 gagcttactc gattggcgac gaatgtcgcc aatttcaaat ttggcatatt tataggcacc
     5881 attttctgaa agatcaccca tttcgcgagc cactttgagg cgcgccatca ccagcactag
     5941 ttctttctgc agaaaatcat accgtcgttg catttcatca tatttggctt tggtaaaggg
     6001 aatggttttc ggtatcatca tttctatttc actgtatatc acagattctc aatcagcgga
     6061 aatgcaactt ttgtgtcaaa atacaaatgt gaaaaaacat tctgtttcta tcgatctcat
     6121 ccgcgttatc gctattcttg gggcattatt cattcatttg acgtatccca tctatgccag
     6181 accagacttt tttggtggta caacttggtg gcttaccacc ataattagcg ctttttctag
     6241 aatatctatt ccattattta ttattctgag tggatatttg ttactaccaa aaaatgaatc
     6301 ttgggcaaca actcaagcta gattggttca tcgcctactc gttccatttg ttttttggtt
     6361 tggtttttat ctaatctggg atcgaatctt cttgcatcga accaattcgt tgcttgagat
     6421 cccaactttg ctgtatcaat cgaatacata ccacctgtat tttttgctta ttctgacggg
     6481 gttgtacttt ttattaccaa tgttgcgttt actttttgtt catctagaaa accattacat
     6541 caaggtttta cttggcgtag gttttatagc gggtgttttg atgtacttcg ttccgtacgg
     6601 tctgatccgc gaccaagcac aatttaatat ttttactgct tgggtgccat atttaggata
     6661 ctttttattt ggcggttttt tggcgaccaa aaaaccgcaa aaacccaggt ggctgggtct
     6721 cgtcacacta tttgcgttca ttaccaccat cgctctcagc gcttccgtag tagcgtttcg
     6781 agaaacaacc actgcctggc tttggcgcag tcacggcgtg gcgtttgttg acgaatatct
     6841 gagcccccaa attattgtct tcgctcttag ctttttttgg ttattggtct ccttgccacc
     6901 caattttaaa ttgctgaata atttgcacgt ctctaaacta ctgcgccaac tagccaaagc
     6961 aagcttcggt gcctatttaa ttcatgtgct ggttttaaat gtactagatt ttaggtttgg
     7021 tctggctctc gacgcttatc cgggaagtct attgcaatac tttttgataa gatcaagtct
     7081 ttgcttactt ctttcatttg gtctaagctg gttattgctt caaattcctt gggtaaaaaa
     7141 agtcttcggc gaaaattaac tttcatttta agcgctcgcg atgtatttga catattttga
     7201 tatactatca gaatgaaatt tactgaagcg gctagactga ccgcacaagc atatttttgg
     7261 tatcactcaa aaaatagtga tacccaatat accaatggac cgatgcatct ttatgatgcg
     7321 gctgccgaat cgctaaccga tcgtaatgct gttggaaaac tagtagcaca attagtggga
     7381 aatcccactg gccgaatctt agaaattgca gccggcactg ggcttatttc acaacacctt
     7441 gcagatgttt cacaattaac ttgcagtgac atttcccagg aatcccttga gctattaaga
     7501 gaacgggtaa cctccgcaaa cattctaagt gctgacttta ccaatctacc ttttagtgat
     7561 cagacttttg atacagtcgt taatgtgggt ggttttcgct atgtcacaga tgaagtagcg
     7621 ttttggaacg aaatggatcg ggtaacctca aaaactggca gagtgatcat tgttcagttt
     7681 tatcccaggt tatccaaatt aagtgggtat gatcttcgag tcgattcgca acatcaaata
     7741 tcattcaaaa aaaacaaaat cttaacccac aaatcaaatg taacgatagg tagtatgtcg
     7801 gttactacgg gtgagtactt attgattgag tttacaaaaa aatagtttgg cattagtcta
     7861 tacactaaat gtcactgaaa tttcagcttc ttcccacacg agtatatttt gaagatcttc
     7921 tgctagaaat taatcatgca aaaaaacaca tctgcatcca aagtttatat tttgatccag
     7981 gacactacct agacaaaatc attaccgctc ttattaaaaa acaaaaggag ggtcttttcg
     8041 ttgagcttat tattgatgca tttggcaaaa gtccccaaat aaaaacccat cgttttgaaa
     8101 gcgcgctcgc aaaactcatc gataatcacg ttgctgtaac atttatcaat cccttacctt
     8161 caagacaaaa ctggatattc ccatatcttc atcgaaatca cattaagatt gtacttattg
     8221 ataacacaat ctattttggt ggaattaatt tttccaacca agaactgacc ttccaagatt
     8281 tgatgataaa agttgtcgac gaaaaaatat cgactatttt tgagcaaata attaatgagt
     8341 caaaacaaca gatattggaa gacagcagca tccgacttga taacaaaaat atactaatgg
     8401 ttgaaggcat caatactcaa tcaattattt atcaacgcgc actatccctc attaacgatg
     8461 caaaaaaatc aattgttttt acctcacaat tttcccctga tggaaaaatg cttgacagtc
     8521 tttatcaatc aactagccgt ggagtaaaac tcacaatcat tgtgccagtt cgcaatcact
     8581 ttaatcgtct atttgaactg ctaaatcagt ggaataactt gatgataatt atcaaacaaa
     8641 aaaatctacc attactcaaa atcaaatcaa tgatccatgc gaagttatta ctaatagata
     8701 accaaacttt gttaattggt tcacacaatt tgtgttggtt gggtgtggca tccaaatcat
     8761 ctgaaatcat gcttcagtca acagacagta atttaatcaa tcaagtcaaa caatttacca
     8821 atcacttaat tactgacaat caaggtgatt catgctgcaa tcactgacaa aagaccgttg
     8881 gattttctat agttccttgt gaacatccca caattggctg atttgatgtc tgacatatac
     8941 catatcctga gattttttta ccaggtagtc tcaacaattt aacgcaacga tttgttgtat
     9001 aacttctaca ggtttaa
//
LOCUS       DAOQZP010000037         8961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_8263_length_8961_cov_5.207950, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000037 DAOQZP010000000
VERSION     DAOQZP010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8961
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8263_length_8961_cov_5.207950"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..589
                     /locus_tag="PKH60_03460"
     CDS             53..589
                     /locus_tag="PKH60_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HNQ17131.1"
                     /translation="MIILILLVILTLTCFVLLFDLLLNILTVVFFKGAFYTRSSNKRL
                     QALQHLLHLSPNDKVVDLGSGKGQVVAMIARNGAKATGIEVNPWLVFISNWHLRRHGL
                     NKQVKIIHSNFWTHDLSKYTVVIVYGIGYMMKHLKLKLERELKPGTKVASVYFPIPGW
                     KTVATEDDVRLYVVPDKG"
     gene            complement(586..849)
                     /locus_tag="PKH60_03465"
     CDS             complement(586..849)
                     /locus_tag="PKH60_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17132.1"
                     /translation="MSQHHRSKFLFKTSARHPRSLAVGRSLANRAALHFQAAGYDVHL
                     HDHVLEFTENLPDGDALDRVYRSLSVNRFVLMGVDPEKNRLAF"
     gene            1054..1125
                     /locus_tag="PKH60_03470"
     tRNA            1054..1125
                     /locus_tag="PKH60_03470"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1087..1089,aa:Glu,seq:ctc)
     gene            1505..2773
                     /locus_tag="PKH60_03475"
     CDS             1505..2773
                     /locus_tag="PKH60_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17133.1"
                     /translation="MNKLLVLSVMAVFGFFLSLGFATPIVVAQESVVLHLFHSEGCPH
                     CKDEREFLSELLPKYPFVQLQLYEVSQNRESAELLSKVGEALETSVAGVPFTVIGDQF
                     IVGFGSSETTGVQIERLIEQQQQSQRPDIVKALQLESTTTKTPATTPTIVEAEKPEEA
                     TQEDGSTVDPTLNVLDTSISVPVVGNISIRSLSLPTLTVLVAFLDGFNPCAMWVLLFL
                     ISLLLGMKDRKRMWILGSTFIVASGLVYLLFMTAWLNFFLLLGYIQLTRTLIGLFALG
                     VGIYQLRDFYVNKDGGCKVVPSEKRRPWLERMKKIVGQKQLVFAMAGMVGLAAAVNVV
                     ELACSAGLPAIYTQVLAVSGLATWQHYVLLLLYIIIFMIDDILIFVIAMKTLQITGIQ
                     HKYARYSHLIGGIVIFLLGILLVFKPEWLL"
     gene            complement(3132..3833)
                     /locus_tag="PKH60_03480"
     CDS             complement(3132..3833)
                     /locus_tag="PKH60_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17134.1"
                     /translation="MSGTLRSAELHMGEYAQKFGVEVRKTKLPARLFDGRLRETQQIE
                     MREVQPALLDYFDTQAQLASLQQLIELFGTPNYQIDGVAAVAFLLNFHVERINREKQR
                     DEATALLLMKEIWDQWQQEIIAAELALQTERASLAVLQTSETETELVKADFESPLVAG
                     VDYAIKEKRKGRFFRQIEINRKQKKEATMRILLLQQQLNSLLSFGSHLSWWWMDTCKS
                     FASMRGPKMIEVPAN"
     regulatory      complement(3957..4087)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4156..4413
                     /locus_tag="PKH60_03485"
     CDS             4156..4413
                     /locus_tag="PKH60_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNQ17135.1"
                     /translation="MPYFVYFLQCADQSLYCGYTKDLSRRIKEHNDSKKGAKYTRTKR
                     PVTLVYFEEHATHSEALKREYQLKQLTNQEKQVLIKSKARL"
     gene            4415..5575
                     /locus_tag="PKH60_03490"
     CDS             4415..5575
                     /locus_tag="PKH60_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ17136.1"
                     /translation="MQVSSEQKKAIEILLKWFADPRRKPFITLGGYAGTGKTTLIAEL
                     KNELFKLNKNCKFAFCSYTGRAAQNLKNRLKLENALGSKDTISTIHGLIYDPIENNQG
                     VIVGWSRKEELEKDLIIVDEASMIDAPIWRDLLSYSIPILAVGDHGQLPPIQGEFNLM
                     AKPDLVLHQIHRQAKDNPIIAVSALARAFGKIPVQSFGSGVVKYSRNDPEAQTLSGEL
                     LAKYTTDTLILCGFNATRVKINQFIRIELGFETPQPTVHDRVICLRNNQTKGIYNGML
                     GTILTIKKEEKDWYQAEIQMDDSPAPFEELIYAPQFGAQTAINFTKNRIQAARGDLFD
                     FGYALTVHKAQGGQAKRVILFEERSQHMTDDMWRKWLYTAVTRAEEELFIFG"
     gene            complement(5588..6751)
                     /locus_tag="PKH60_03495"
     CDS             complement(5588..6751)
                     /locus_tag="PKH60_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNQ17137.1"
                     /translation="MQYISMPLENLHNQLTQLLDYPDKHASEQTPKPSECEIVGIPSF
                     IRQFRDEASRASARVWLQTMYLFPSHFSDLLTNALMRSGDKHIDTRLYTDGIQHPLNF
                     RDKFYAKMFGTISERTHQHKATELQKNEFQVHNVKTIESRPFGLINYALFSYLRNHFK
                     LAIVDNTVWIGGLNAGKDSDYNRIDFMARFKSPGLVDAVANIVENQQCLTENVNLETD
                     GYNLLVDAGLPGDSIIYTSVLNRLKNIQDPKHTEVILLSPWVPDKELLQELHQLHLKG
                     AKIQVLTSYHKFQIAFEGIYAVIKNFNNLMMKLKKLEIPILYTPLEVHGKMLLIDEGE
                     QTHALITTHNLVTQGVKMGTAEIAVGSSKPDFVSQCKRFLDKVAEASSQVRSA"
     gene            complement(6757..7578)
                     /locus_tag="PKH60_03500"
     CDS             complement(6757..7578)
                     /locus_tag="PKH60_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNQ17138.1"
                     /translation="MQKVVFFDLDGTLWDSRHPLLQTAWDRLPQAWTTIGGEIPNGFN
                     WSQLAGSTEYAVVKQLVDMQFPTIDESIKEQLIQQTISEMDRHYFEHAPTDISPALYN
                     DTQRGLQHLRDQGWTLGIITGNSEPVARHKLQAAGLLQFFSTDPKLLYFGENYKSRAE
                     MMKEAWVQSMIFGHERPKVPPLIYVADTLRDLTESSEAWWRQSSNYLSLEYRILLRLQ
                     PAVYPYIREINNFNNGYKDITAQLFETIGDDHFTQTVRRESRFDWVSARELERGW"
     gene            complement(7606..8277)
                     /locus_tag="PKH60_03505"
     CDS             complement(7606..8277)
                     /locus_tag="PKH60_03505"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HNQ17139.1"
                     /translation="MKNNIATVPKQGKNKGRLFRVLPLSTLFGILLLLFGAGNLGYEY
                     GPLLIAETRYTIKQSAPVQVQQVFFNEVATQKALEPVDTDFGILIPKIEANAPIVAEV
                     DPYDSQIYQRELTKGVAHAAGTGLPGEDKTMFLFAHASGDIAMARRYNSIFYLLNKME
                     PNDEITIFYRGAPYKYQVSQVKTVNPDAVDYLENNSDTDLILMTCTPPGTTWKRLLVF
                     ATLHR"
     gene            complement(8267..8731)
                     /locus_tag="PKH60_03510"
     CDS             complement(8267..8731)
                     /locus_tag="PKH60_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17140.1"
                     /translation="MITIPIGPNQEYTLTTSKPNCDDTYYYVIRAFDVNGNASDLVGD
                     LEIKTVIIESASGTTQTVQLGAIPAGSAFIAGGSTTDETAEGTTGEGEEEIDEPETTA
                     EPTETESTEGTVLGSATETLMSFLTRFGLPIGVGLIVLAGLYIIFFGKRNEK"
     gene            complement(8728..>8961)
                     /locus_tag="PKH60_03515"
     CDS             complement(8728..>8961)
                     /locus_tag="PKH60_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17141.1"
                     /translation="YDFRVVVSGDGPDKISDEVRIGLTPIDQTNRLTTVKTQVITVKI
                     KLVLKQPMMVKPPVLKSIAARTMITLPPNRTPE"
BASE COUNT         2468 a         1783 c         1952 g         2758 t
ORIGIN      
        1 gtaaatgggc ttattggaaa ttagaaaaat atatgtcata ctctttcata caatgatcat
       61 actcatatta ctcgtaattc tcaccctaac ttgttttgtc ttgttatttg atttgctgtt
      121 gaatatttta acagttgttt tctttaaggg cgctttttat acacgttcta gtaacaaacg
      181 cctgcaggcg ctccagcact tactccatct ttctccaaat gataaggttg tagatcttgg
      241 ttctggtaag ggacaggttg tggcgatgat tgctcgaaat ggtgcaaaag caacaggaat
      301 agaagttaat ccatggttgg tgtttatttc taattggcat ttacgaagac atggtctgaa
      361 taagcaagta aaaatcatac atagcaattt ttggacgcat gatttgtcaa aatacacagt
      421 tgtgattgtc tatggcattg gctacatgat gaaacatctc aagttgaaat tggaacgtga
      481 actaaaacca ggcaccaaag tggcatcggt ctactttcct ataccgggtt ggaaaacggt
      541 tgcgacggaa gatgatgttc gtctgtatgt agtgccggat aagggttaaa aagctaaacg
      601 atttttttct ggatcaactc ccattaacac aaagcgattg accgagaggc tgcgatacac
      661 tctatcgaga gcgtctccgt ctggtaaatt ctcagtaaac tctaaaacat gatcatgtag
      721 gtgaacatca tagcctgctg cttgaaagtg cagtgctgcg cggttagcaa gcgaacgacc
      781 aacggcaaga gaacgagggt gacgagcgct agttttaaag agaaacttag aacggtgatg
      841 ttgtgacatg tggcaactcc tataaaaaat tatgttgcta ctaaaacaag tatagtttgg
      901 tggatcgaat tactcaatca ataccaagtg tgtaccaatc aaaactattc atcattgcca
      961 actaataata gtacgttcca tttatcctaa ttatttcaaa attgaaagtc agacttagta
     1021 ttagatttgg tatactacgc cattgccagc aatggccccg tcgtctaacg gttaggacac
     1081 agggttctca tccctgtaat tggggttcga ttccccacgg ggtcacaaat gaaacgctca
     1141 cgcccaatac ctcagcgtat caatgacctg ctaccaactt actttgcttg tttcgatcat
     1201 gaggcagcag ctgcaggctc acatcaaaaa tgttttgctt acgttacaag atggacttgt
     1261 cggcatccag tacctttcga gtaataacca gggtaccttt tggatgcttg cgctgatagg
     1321 tgtttttctc tgttttctga ttagtcctcc gtacgagtat cgtgtaaata atgtagacta
     1381 ttggcgagct gaaattctta ccttaattaa aggaacgcct tagggtaaaa ctcatggtct
     1441 caggcgtatg ggtaccactc taaggctttt taaattttga atttgaggag tatgatattt
     1501 tgagatgaat aaactactcg tattgagtgt aatggcagta tttggatttt tcctcagttt
     1561 ggggtttgcc actccaatag tagtggcgca agaatcagtc gtacttcact tattccactc
     1621 tgaagggtgc cctcactgca aagatgagcg ggaatttttg tcggaattgt taccaaagta
     1681 tccattcgta cagttgcaac tatatgaagt ttctcaaaac agggagtcag ccgagttgct
     1741 tagtaaggtt ggcgaagctc ttgaaacctc agttgctggc gtgccgttta cggtgattgg
     1801 tgaccaattt attgtgggct ttggtagttc agaaactacg ggagttcaga ttgagcggct
     1861 tattgaacaa caacaacaga gtcaacgacc agacatagtg aaagctctac agctcgaatc
     1921 tactacaact aaaacgcctg cgacgacacc tacgattgtg gaggctgaga aaccggagga
     1981 ggcaactcaa gaagatggtt caaccgtaga tcccactctt aatgtgttag atacatctat
     2041 ttcagtaccg gtggttggga atatttcaat tcgctcattg tcacttccca cgctgaccgt
     2101 gttagttgct tttctggatg gttttaatcc ttgcgcgatg tgggtgttat tgtttttgat
     2161 tagtttacta cttggcatga aagacagaaa gcgcatgtgg attttaggtt ctactttcat
     2221 tgttgcttct ggattagttt atctactatt tatgactgca tggttaaact ttttcttgtt
     2281 gcttgggtat atccaactga ctagaacgtt gattggtcta tttgcgttgg gagtgggtat
     2341 ctaccaactg cgagattttt atgttaacaa ggatggcggt tgcaaagtag ttcccagtga
     2401 aaagcggcgg ccttggttag aacgaatgaa aaagatagtg gggcaaaaac agttggtttt
     2461 tgctatggct ggaatggtgg ggttagcggc agcggtcaat gtcgttgaat tggcttgctc
     2521 ggcggggctg ccagcaattt atactcaggt tttagcggtc tcaggattgg caacatggca
     2581 gcactatgtc ttgctactac tctacatcat tatttttatg attgatgata ttctcatttt
     2641 tgtgattgcc atgaagacct tgcaaattac aggaattcag cataagtatg ctcgctactc
     2701 ccatttaatc ggtggaatcg taatcttttt acttggaatt ttgttggttt ttaaaccaga
     2761 gtggttgctg tagagcatgc ccattgacat caggtttcag atataacaag ataagataga
     2821 taaatagtac ctgctttgtt ttagatatgt caataacccc aaagaaagta tttttttttg
     2881 cgactattat tatttcttta gctttaattg gaagatctac tagatttaat tacaatgatg
     2941 gatcctttca tatatctcaa tgtcaagcgc ctcttttaaa cgacacggag tgtaaatatt
     3001 cggtgtggaa gctggaagga ggcatgttaa gagcggggtg tatccatttg tggttgtagg
     3061 tatagagcgt gtgatgtata gcttccaaga aatgtccttc tgattcaaaa cgatcaaatt
     3121 caagaccaag atcagttagc tggaacctca atcattttag gtccccgcat cgaagcaaac
     3181 gatttgcagg tatccatcca ccaccaagag agatggctgc caaaactcaa taaactattg
     3241 agttgttgtt gcagtaatag tatgcgcatt gttgcctctt tcttttgttt cctatttatt
     3301 tctatttgtc ggaaaaatct accttttcgt ttttccttga tggcataatc cactccagca
     3361 actaatggag attcaaagtc agctttgact aactcagttt ctgtctcgct cgtttgtaga
     3421 actgcaagtg acgctcgttc agtttgtaag gcaagctctg cagcaattat ctcttgttgc
     3481 cactgatccc aaatttcttt catcagtaac agcgcagtcg cttcatcacg ttgtttttct
     3541 cggttgatcc gctcaacatg aaaattgagc aagaacgcta ccgccgcaac gccatcaatt
     3601 tgatagttgg gtgttccaaa caactcaatc agctgttgca gcgatgcaag ctgtgcttgt
     3661 gtgtcaaaat aatctaacaa ggctggctgg acctctcgca tctcgatttg ttgtgtttca
     3721 cgcaaccttc catcaaataa cctggctgga agcttggttt tacgaacctc aactccaaat
     3781 ttttgagcgt actcgcccat atgcaactct gcgcttctca gagtacctga catagatttc
     3841 ctttcctcac ctgactgtga gaaatactac gacaagcaaa caaagcaacc aacagctaat
     3901 tatcaaaaaa cagagaagat aaaaaagtgc tgttcatgtt tcgatgctcg tcgatgctac
     3961 aacacgctga tgaagatcgg acttccagat tgtttcgttg taacaactgg ttaccgttgc
     4021 ggcacagttg cagattttca ctgcattcca cacgagcgat taaaaaatct tatacgactt
     4081 tggaagtacg tgcaactact attttttagt ttgggttgca cagtagatga ttgttgtaca
     4141 tggtatagtc tccctatgcc atattttgtc tattttctcc agtgtgcgga tcagtccttg
     4201 tactgtggct ataccaaaga tttgagcaga agaattaagg aacacaatga ctcaaaaaaa
     4261 ggggcaaagt atacccggac taaacgccca gttacgctgg tgtattttga agagcatgct
     4321 acccacagcg aagcgctaaa acgtgagtat caacttaagc aattaactaa tcaagaaaaa
     4381 caggtcctga tcaaatcgaa agctcgtttg tagtatgcaa gtatcatcag aacaaaaaaa
     4441 agcgattgaa attctgctca aatggtttgc cgacccacgc cgaaaaccgt tcatcacctt
     4501 gggtgggtat gcgggcactg gcaaaacgac cttgattgct gaattaaaaa atgaactgtt
     4561 taaactgaat aaaaactgta aatttgcgtt ttgcagttac actggccgag cggcgcagaa
     4621 cttgaaaaat cgacttaagc ttgaaaatgc attgggtagc aaggacacaa ttagcaccat
     4681 tcatggatta atttacgatc cgattgaaaa caaccaaggt gtaattgtag gttggtcgcg
     4741 caaagaagaa ctagaaaaag acctcatcat cgtggatgaa gcttctatga tagatgcacc
     4801 gatttggcgc gacctacttt cttacagtat tccaatttta gcagtaggag atcatggtca
     4861 attgccaccc atccaaggcg agttcaactt aatggcgaaa ccagatttgg ttttgcatca
     4921 aattcatcgt caggccaaag ataatccgat catcgctgtg tccgcgctgg caagagcgtt
     4981 tggcaaaatt cccgttcaat catttggctc tggcgtggtg aagtattcac gtaacgatcc
     5041 tgaagcccag acgctgagtg gagagctgct ggcaaaatat actaccgata ccctgatttt
     5101 gtgtggcttc aatgccacca gagtaaagat taaccaattt attcgtattg agttgggttt
     5161 tgaaactcct caacccacgg tgcatgatcg agtaatttgt ttgcgtaata atcaaaccaa
     5221 ggggatttac aacggcatgc tgggtactat tctgactata aaaaaagaag aaaaagattg
     5281 gtatcaggca gaaattcaaa tggatgatag tcccgctcca tttgaggagc tgatctatgc
     5341 gccccaattt ggggcgcaaa ctgccattaa cttcaccaaa aaccgcatcc aagccgctcg
     5401 cggcgacctc tttgattttg gctacgcact aactgtacac aaagctcaag gtggtcaagc
     5461 caagcgcgtt attttatttg aagagcgttc tcagcatatg actgacgaca tgtggaggaa
     5521 atggctctac acagccgtaa caagggcaga agaagagttg tttatttttg ggtaattcgg
     5581 ctggtagcta tgcacttcga acctgagagg atgcttcagc aactttatcc aagaatctct
     5641 tacattgaga aacgaagtct ggttttgatg acccaacggc aatttctgca gtacccatct
     5701 tcacgccctg agtgactaga ttgtgagtag taattaatgc atgtgtttgt tcaccctcgt
     5761 caataagtaa catttttcca tgtacttcta agggtgtata gagaattgga atttccagct
     5821 tttttagttt catcatcagg ttattaaaat tctttattac cgcatatatt ccttcaaatg
     5881 caatttggaa tttatgataa cttgtgagta cctgaatttt tgcgcctttt agatgcaatt
     5941 gatgtagttc ttgaagtagt tctttatctg ggacccatgg actcaagagg ataacttctg
     6001 tgtgtttagg atcttgaata tttttcaatc tattcagaac gcttgtataa atgattgagt
     6061 cacctggtaa tccggcatcg actaataagt tgtaaccgtc ggtttctaag ttgacattct
     6121 cagttaaaca ttgttgattt tcaacaatgt tggcaactgc atcgacgagt ccgggtgact
     6181 taaatcttgc catgaagtct attctattgt aatcactatc ttttccagca tttaacccac
     6241 ctatccagac tgtgttatca acaatggcga gtttaaagtg atttcttaaa taagaaaaca
     6301 gtgcatagtt aattaacccg aacggtcttg attcaatagt ttttacattg tgaacttgaa
     6361 attcattttt ttgtagttcg gttgccttgt gttgatgtgt tctttcagat atagtaccaa
     6421 acatttttgc ataaaattta tctctaaaat tcagtggatg ctgaattcca tctgtataca
     6481 atcttgtatc gatatgttta tcaccacttc gcatgagagc gttggttagc aaatcagaaa
     6541 aatgagaagg aaataggtac atagtttgta accatactcg tgctgaggct cttgaggctt
     6601 catcgcgaaa ttgacgaata aaacttggga tacctacaat ttcgcactca gaaggttttg
     6661 gagtctgttc tgaagcatgt ttgtcggggt agtcaagtag ttgagtaagc tgattgtgta
     6721 aattttcgag tggcatagat atgtattgca tatattctac caacctctct ccaattctct
     6781 agctgaaacc cagtcgaatc tgctttctcg tcttacagtc tgagtaaagt ggtcgtctcc
     6841 aatagtttca aatagctgtg ccgttatgtc tttatagcca ttgttgaaat tatttatctc
     6901 ccttatatag ggatacacgg cgggctggag tcgtagaaga atgcgatatt caagactgag
     6961 atagttacta ctttgtctcc accaggcttc actactttct gttaaatctc gtaaagtatc
     7021 agcgacataa atgagtggag gtacttttgg tctttcatga ccaaaaatca tcgattgcac
     7081 ccatgcctcc ttcatcattt cagctctact cttgtagttt tcaccaaaat aaagcaattt
     7141 tggatcagta ctgaagaatt ggagtaatcc agcagcctgc aatttgtgtc tagccactgg
     7201 ttctgaattg ccagtaatga tgccaagggt ccagccttga tctcgtaaat gctgtagtcc
     7261 tctttgagta tcattgtaca aagcggggct aatatcagtt ggtgcatgct cgaaatagtg
     7321 gcggtccatt tcagaaatag tttgctggat gagttgttct tttatacttt cgtcaattgt
     7381 tgggaattgc atatcaacca gttgttttac aaccgcatat tctgtactac cagcaagttg
     7441 agaccagttg aaaccatttg gaatttcgcc accgattgta gtccaggctt gaggtaatcg
     7501 atcccaagct gtttgaagca gtgggtgtct tgagtcccag agagtgccat ctaagtcgaa
     7561 gaataccact ttttgcatag ttggattaat tagaattgag aattattatc tatgtaaagt
     7621 ggcgaacacg agtaatcgct tccaagttgt tcctggtggg gtgcaggtca taagaatcag
     7681 atctgtgtct gagttatttt ctaagtagtc aacggcgtct ggattgacgg ttttgacttg
     7741 agagacttgg tatttgtagg gcgcgccgcg gtagaaaata gtgatttcat cattgggttc
     7801 cattttgtta agcaagtaaa agatagaatt gtagcggcgc gccatcgcaa tatcaccgct
     7861 ggcatgggca aataaaaaca tggttttgtc ttctccaggc aggccggttc cagcggcatg
     7921 agccactcct ttagtcaact ctctttggta aatctgtgag tcatagggat ctacctcagc
     7981 cacaatcggc gcattggctt caatttttgg aattaagata ccaaaatcag tatctactgg
     8041 ctcaagtgct ttttgagtag ctacttcatt gaagaatact tgttgaacct gcactggcgc
     8101 agattgctta atggtatatc gcgtttctgc aataagcagt gggccgtatt catagcctag
     8161 gttaccagcg ccaaaaagca ataataaaat tccaaagaga gtgctcagtg gtaatactct
     8221 aaacaatcga cctttgtttt taccttgctt tggtacagtc gcaatgttat ttttcattcc
     8281 gcttgccaaa gaagatgatg tagaggccgg ccagcacgat cagaccaaca ccgattggta
     8341 agccaaatct ggtcaggaat gacatcaatg tctcggtggc cgaacctaaa acagtgcctt
     8401 cagttgactc tgtttctgtc ggctcagcgg tagtctctgg ttcatcaatt tcttcttcgc
     8461 cttcgcccgt tgttccctcg gctgtttcgt ctgtggtaga tcctcctgca ataaaggctg
     8521 agccagcagg aatggcgccc agctgaacag tctgggtggt accactcgcg gattcgatga
     8581 tcactgtttt gatttctaga tcgccaacca agtcagaggc gttgccgttg acatcgaagg
     8641 cacgaatcac gtagtagtag gtatcatcac agtttggctt agatgtggtc agggtatact
     8701 cctggtttgg tccaattgga atagtaatca ctctggtgtc cggtttggcg gtaaagtgat
     8761 cattgtccgt gctgcgatag acttcaacac tggaggtttg accatcattg gctgttttaa
     8821 aaccaatttt atcttcacag taattacctg ggtttttact gtagttaacc ggtttgtctg
     8881 gtcgattggt gtcagaccaa tgcgcacctc atcgctaatc ttatctggtc catcgccact
     8941 gactaccact cggaagtcgt a
//
LOCUS       DAOQZP010000038         8731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_8533_length_8731_cov_5.062817, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000038 DAOQZP010000000
VERSION     DAOQZP010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8533_length_8731_cov_5.062817"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="PKH60_03520"
     CDS             <1..931
                     /locus_tag="PKH60_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17142.1"
                     /translation="TYYLRLFNKREYFNENQALLAKFYTNIPMEFLRQLFLNSTEALN
                     PDFLFINGAGHGSHDIVGIGKFYSVMLPLLILGIAALFSRHSLFTSNGRWLVIAWLVV
                     GLLPAIITWQPAHATRSYAVIIPLILLTTQGCFWLYQNIRKRPEVIQLSTVLIFLIIL
                     VYQIYSMLVTYYIVAPQRDIDNWTWYAKDMTNFLSDAKNRVDHVYVEGNSWSPYIFYL
                     FYAKINPSIAQERLVHLQPDLEGFRHVSSFDTIEFGDVPFQHLLEAGDSYAIFTPLSQ
                     LPAGFSQDPQQFDVYQVFSNTHSKESYVAIIKN"
     gene            909..2633
                     /locus_tag="PKH60_03525"
     CDS             909..2633
                     /locus_tag="PKH60_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17143.1"
                     /translation="MWQSLKTKLLVNQSIFALAILIGICLRLFSFGQIPVGTYWDETA
                     ILIDATSIATTGRDMHGNHWLQALYPSYGDYKLPVYIWLASIAVKVVGTNELAVRLPS
                     LIVGLATIILAAALAGELAKYWPKQLRQTFQLAAASVVSLSFWAIMFSRIGFEGHLGQ
                     LWLGLSFLSLLKSKAKFLWWPIAGIFGALALFTYYSTRFIWPALWLLFIIPVIISILT
                     EPNKRRQLFLGQIVPAFSSLLIFGGFLLLMVRSPLYPASEIFRLSSDSILNQAPFVAQ
                     SNLSQLIEGPSGIGRIVLHRYVFQIKALASHVASHLSLDYLFFTGDDNLRHGTGFHGL
                     FPMLFAPVLLIGIIGLFKHHKKVFVFLLFWWLTSLLPASVPYDVPHALRSLNSLLPIS
                     LFIAYGVTTLYLATTKNIALLFKIISFGVLIESIAFAYYYFTVYPITSAAEWQSGYQQ
                     LAQHAIDHRDAVNQFWINAGDGRIYLWLLADGAVKPQDLSSLAWKGYQLDQIENILFT
                     DFVYTYEGFPNTPFALAGRRKEIISNLAILKATPQLVVPILDKNNQEQFVVTYFNLSK
                     DKLKDVSE"
     gene            2620..4209
                     /locus_tag="PKH60_03530"
     CDS             2620..4209
                     /locus_tag="PKH60_03530"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HNQ17144.1"
                     /translation="MFQNKVLAISLLGIFLLASFFRSFKINSVVPVLNRDEAALGYNA
                     LLLKDTGRDEWGLRWPISPKSFGDSKLIGYPILLVGLFHLLPADDWVVRFPAILAGSL
                     LPILVFFFARTLGISRRFALLAALSVAVNPVFVHFSRFAYEAMVALTYFVTATTLVLH
                     QTKNFQLRIFFDLVATLLFFASLLTYNTPLILLPLFIPLLIVHRGILPVRKWLPTAII
                     LSLTWLLAIALLFPMLSQKSGIAIFSDQTYIQNYPQYRASFIPPLQPILGNSYVYFAT
                     AIFKHAIQSFSPNFLVLNGGSHPWHSLVKYGHISWIIYFGAIMGIVTTAIAWLKHRKR
                     ISKNVLFLILFILVSLVPASITVDAPHATRSLLFFVILVVMASYFYQLLLAKVRLPIF
                     GQYLKQLVFCILLITTLIGGLSFFTKYLGEYPSQAKRVYQAGFHDIISTLDALYPYTP
                     VAVVDPGGFQYILLAWYNKISASNFSQSIIKQQPNTIGLQYGERLGRYHFIVQANDRS
                     VEETIVLFWDEQGNEWQYQTL"
     gene            4206..5012
                     /locus_tag="PKH60_03535"
     CDS             4206..5012
                     /locus_tag="PKH60_03535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ17145.1"
                     /translation="MKTTTCKLSVVLAIYNEAANLARCLEAVVNIADEIIIVDGNSTD
                     DSVKIAQSFQAKVISTTNKANFHINKQKAIDAARGQLILQLDADEVVAEPLATFILKI
                     KKQPDKYQNISAWWLIRQNYFLGKWLKKGGQYPDPVIRLFWRGKAELPQKDVHEQMTV
                     RGMVGWADGYLEHYSNPTLTEYLRKMNTYTSFKAQQLYQDKINPTVVNTLLYFFYYPS
                     RTFISLFLRHKGILDGWAGFLFSLLSSIHYPIQYVKLWELHEKSRLNNLR"
     gene            4981..6039
                     /locus_tag="PKH60_03540"
     CDS             4981..6039
                     /locus_tag="PKH60_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNQ17146.1"
                     /translation="MKNPDLTICVKLDTSPLNSGHVFRGIGVYTRLVSEYLAKVSDIT
                     VITAKSDKQKVDLVHYPFFDLFFPTLPIKPWLKTIVTIHDVIPLVFPKQYSPGKKGAL
                     MLKRQRVALTFVSTIITDSDASKEDIHRYLHVPLEKIQVVYLAGQPELQPATEIEIKH
                     LKKRLGLTKPYALYVGDINYNKNIPQLIKMLKFLPPNIELVCFGKAMKQSDIPEWQAI
                     ITQAALSNVENRVHFITDIDSNDFQSLSALYGGALCYVQPSLYEGFGLPVLEAMQCAT
                     PVVSTNTSSLPEIVGKNGILVDPEAEALATGVLSIYQLATSDRQKIVEKGLKHARTFS
                     WNKTAQLTAEVYRRALGR"
     gene            6036..7727
                     /locus_tag="PKH60_03545"
     CDS             6036..7727
                     /locus_tag="PKH60_03545"
                     /inference="COORDINATES: protein motif:HMM:NF021635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HNQ17147.1"
                     /translation="MKRLLFVAIILILFFTRSFWQNEGIPYTHDGENHLARFANFKVA
                     LKEGQLPPRFAPNLLNHYGYPVFNYNYPLANIISAPFSVLKFHYELTFKLIIFFALLL
                     GSLGVIRWLTQYSSKKLPILAALIAYLSGPPLISALLFRGNIGEILAYSLWPWLFFSI
                     SEATNKLSSKRILMFIFVWTAFLLSHNISVFIAAFVFLIYGVWQLLVTKERRRFASQI
                     ATIIIASIGLSLWFWLPAIAEMKEVVVSQAANQREYLLHFPTLKQLLYSPLTFGYSYP
                     GPVDTLSFWLGWTIPFSLLLVSAIFLANSFSKQDKFKKILNYFRSHPLGIYVLLLLLS
                     LFLQLEISSTIWQLLPNFRLIQFPWRFGMLSTIVSLPVLVWLLQKNRQLITCGIWLVL
                     FVQLFLVFQAHPIDYVTKQKSEYDLHGGTTSTQNENVPFTFRYLLIADWQPSPSVFSG
                     EAEITKLEYWNGSDRLYRVNVVTPTVLVEPTMYFLGWETQIRNVVTSSDSFKNVTYID
                     SDEIQGRIAYRLEPGIYQVHTRFTQNTPARHIGNASFVITGIALSGLLLARKYRL"
     gene            7760..8680
                     /locus_tag="PKH60_03550"
     CDS             7760..8680
                     /locus_tag="PKH60_03550"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNQ17148.1"
                     /translation="MNPQPLRIIVTGGSGFLGHHLIRTFVQAGHQVRNIDLRPNPEFE
                     TALADVRDQNKMNELITDADVVFHLASLIEAGESVKESAKYFDNNVMGTLSVLEAMRR
                     NQIKRFIFSSSAAIYGEPMRTPISEDDRTLPINPYGVTKLAMEGLLRSYVESYSFTGM
                     ALRYFNLYGPEEHHVPETHAIPRFIQQITQDEEVTLWGDGGHLRDYVYIHDIVDAHLL
                     ALDYAIDHPNQYHYFNLSAEQPASVLEVIKHLENSLNKKANIKHFPDRPGDPRVLIAD
                     ATKAHQQLGWHAATTLSEGLKKTVQYFTSL"
BASE COUNT         2421 a         1855 c         1628 g         2827 t
ORIGIN      
        1 aacgtattat ttgcgcctat tcaacaaacg agaatatttc aacgagaatc aagctcttct
       61 agctaagttc tataccaata taccaatgga atttcttaga cagctttttc taaatagcac
      121 tgaggcatta aacccagact ttctctttat caatggcgcc ggtcatggtt ctcacgatat
      181 tgttgggatc ggaaaatttt atagtgtcat gttgccgtta ctcatactag gcattgctgc
      241 tttgttttct cgtcattctt tattcacctc taatggaagg tggttagtta ttgcttggct
      301 agtcgttggt ttattgccag ccattattac ttggcaacca gcgcatgcta ctcgctcata
      361 cgcagtaata attcccctca ttcttttaac aacccaaggc tgtttttggt tatatcaaaa
      421 tattcgcaaa agacctgagg taatacagct gtcaaccgta ttgatatttt tgataatttt
      481 ggtttatcag atttatagta tgttggttac gtattatatc gttgcccccc aacgcgacat
      541 cgataattgg acttggtacg ccaaagatat gaccaatttt ttaagtgatg caaaaaaccg
      601 agtagatcat gtgtatgtcg aaggtaattc ttggtcgcca tacatctttt acctattcta
      661 cgcaaaaata aaccccagca ttgcccaaga acgactggtt cacttacagc cagaccttga
      721 aggctttaga cacgtgtcga gtttcgatac aattgaattc ggtgatgtac cgtttcagca
      781 tttgcttgag gccggtgatt cttatgctat tttcaccccc ctcagccagc tgccagccgg
      841 ttttagccaa gatccccagc aatttgatgt gtaccaagtc ttttcaaata ctcacagcaa
      901 agaatcctat gtggcaatca ttaaaaacta aactactcgt taatcaatct atatttgctc
      961 tagcaatctt aataggtata tgtttgcgct tgttcagttt tggacaaatt cctgtgggca
     1021 cctattggga tgaaacggct atcttgattg atgcaacatc tatagctacg actggtagag
     1081 atatgcatgg taatcattgg ttgcaagcgc tgtatccctc ctatggtgac tataaattac
     1141 cggtttacat ttggctagcc agcattgctg taaaagttgt tggcactaat gaacttgctg
     1201 ttcgcttgcc aagcctaatt gttggattgg ctacaattat attggcggca gctcttgcag
     1261 gagaacttgc taaatactgg ccaaagcagt tacgtcaaac gtttcagcta gctgccgcca
     1321 gcgtcgtctc actttctttt tgggcaatta tgttttcccg aattggcttt gagggccatc
     1381 taggacaatt gtggctgggt ctctcatttt tatctttgct caaatcaaaa gccaaatttc
     1441 tttggtggcc aattgcggga atctttggcg cgctggcttt gtttacctat tattctactc
     1501 ggtttatttg gccagcactt tggttgctat ttatcatccc agtcatcatt tcaattctaa
     1561 ctgaaccaaa taaacgcaga cagctttttc tcggtcagat agtacccgct ttttcatcct
     1621 tgctcatttt tggtgggttt ttactactaa tggtacgctc acctttgtat ccagccagtg
     1681 aaatatttcg cttaagttcg gactccattc tgaaccaagc tccatttgta gcacaatcaa
     1741 atctatcgca gctaatagaa ggtccatctg gcattggtcg aattgtttta caccgatatg
     1801 tgtttcaaat taaagcctta gcaagtcatg ttgccagcca tctttcgttg gattacttat
     1861 tttttactgg agatgacaat ctgcgacatg gcactggttt tcatggttta ttccccatgt
     1921 tatttgcacc tgttttgctg ataggaatca tcgggctgtt caaacatcat aagaaagtct
     1981 ttgtcttttt attattttgg tggctgacta gtttgctccc ggcatcagta ccatacgatg
     2041 ttccccatgc cctccgatct ctcaatagtt tgttaccaat ttcacttttc atagcctatg
     2101 gtgtcacgac actctattta gcgaccacaa aaaatattgc gttgttgttt aagattatta
     2161 gctttggagt tttaatagaa tcgattgctt ttgcctatta ttattttact gtgtatccaa
     2221 ttacctcagc tgccgaatgg caatcaggtt atcagcaatt agcccaacat gcgattgatc
     2281 atcgcgatgc agttaatcaa ttctggatta acgcgggtga cggccgaata tatttgtggt
     2341 tattggccga tggcgctgtc aaaccccaag atttatcatc gctagcatgg aaaggttatc
     2401 agttggacca gattgaaaat attctcttta ctgactttgt atatacctac gaaggttttc
     2461 caaacactcc atttgcacta gctggtagaa gaaaagaaat tattagcaat ttggctattt
     2521 taaaagctac tccacaattg gtggtaccca ttttagataa aaataatcaa gaacagtttg
     2581 tagtcacgta cttcaatctt tccaaggata aactcaaaga tgtttcagaa taaagtactt
     2641 gcaataagtt tacttggcat cttcctgctt gctagttttt ttcgttcatt caaaataaat
     2701 tcagtggtgc cggttcttaa tcgtgatgaa gctgccttgg gttacaacgc gcttttgctt
     2761 aaggacactg gccgtgatga atggggacta cgttggccga tttcgcctaa atcttttggc
     2821 gacagcaaat taattgggta tccaatactt ttagttggtt tgtttcattt attacctgct
     2881 gatgattggg tggtacgttt tcccgctatt ctggctggtt cattgctacc catacttgtc
     2941 ttcttttttg cgagaactct gggcatatcc agacgattcg cgctccttgc ggcactcagc
     3001 gtggcagtca atcctgtgtt tgtgcatttt tctcgctttg cctatgaggc gatggtagcg
     3061 ttaacctatt ttgtaactgc caccacttta gtacttcatc aaacgaaaaa ttttcaactt
     3121 agaatctttt ttgatctggt cgctacgcta cttttcttcg ctagcctact cacatacaac
     3181 actccgttga tacttctacc tttatttatt ccattgttga ttgtgcatcg aggtattttg
     3241 ccagtgcgta aatggctacc cacagcaatc atactcagct tgacttggct gttagcaatt
     3301 gctcttttgt tccctatgtt gagtcaaaaa agtggcatcg ctatattctc agatcaaacg
     3361 tatatccaaa actacccaca gtatcgagct tcatttattc cgcctctcca accgattctg
     3421 ggcaactcat atgtctattt tgctaccgct atttttaaac atgctattca aagcttttca
     3481 ccaaactttt tagtcctcaa tggtggatcg catccttggc actcacttgt gaagtatggt
     3541 catatcagct ggatcatata ttttggggcg attatgggaa tagttacgac agccattgct
     3601 tggctcaagc atcgaaagcg gatttctaaa aatgttctgt ttctaattct attcatacta
     3661 gtatcattgg tacccgccag tattaccgtc gatgcacctc atgccacacg tagtctttta
     3721 ttttttgtaa tactagtcgt aatggccagt tacttctatc agctgctgct agcaaaagta
     3781 cgcttgccaa tatttgggca gtacctaaaa caattagtgt tctgtatatt attgataacg
     3841 acattgattg gtggactatc attctttacc aagtatcttg gagaatatcc aagtcaggcc
     3901 aagcgggttt atcaagccgg atttcatgac ataatttcaa ctctagatgc actctatcca
     3961 tatacaccag ttgcagtggt agatccaggt ggttttcagt atatattgct ggcttggtac
     4021 aataaaattt cggcttcaaa cttttctcaa tcaatcatca agcaacaacc caacacaatt
     4081 ggtttacaat atggtgaacg tttgggtcga tatcacttta ttgtccaagc aaacgatcga
     4141 tcggttgagg aaactatagt actattttgg gatgaacaag gtaatgagtg gcaatatcaa
     4201 acactatgaa aactactact tgtaagctct cggttgtttt agccatctat aatgaggcag
     4261 ccaacttagc tcgatgttta gaagctgttg tcaatattgc cgatgaaatt attattgtcg
     4321 atggtaattc aaccgatgat tctgttaaga ttgcacaatc atttcaggcc aaagtaattt
     4381 ctactaccaa taaagccaac tttcatatta ataagcaaaa agccattgat gcagcgcgag
     4441 gtcagttaat tcttcaactc gatgctgacg aagtcgtggc cgaaccccta gctactttta
     4501 tattgaaaat taaaaaacaa ccagacaaat atcaaaacat ctcagcttgg tggttaattc
     4561 gtcaaaacta ttttctcggt aagtggttaa aaaaaggcgg tcaatatccc gatccggtaa
     4621 ttcgattatt ttggcggggt aaagcagaat tgccacaaaa agacgttcat gaacagatga
     4681 cggttcgtgg tatggttggt tgggctgatg gttatttaga acactatagc aatccgactc
     4741 tgactgagta tctccgaaaa atgaacacct ataccagctt caaagcccaa cagctatatc
     4801 aagataaaat caatccgact gtcgtaaata ccctgctata ttttttctat tatccatctc
     4861 gcacgtttat ttccttattt ctacggcata aaggaattct tgatggctgg gcggggtttc
     4921 tcttttcgct cttaagtagc attcactatc caattcaata tgtaaaacta tgggaactgc
     4981 atgaaaaatc cagacttaac aatttgcgtt aaacttgaca cttcacctct caattcaggt
     5041 catgtgttca gaggcatcgg tgtatacact cgtttggtat cggaatattt agcaaaagta
     5101 tcagacatta ctgtaattac agcaaaatca gacaaacaaa aagtagattt ggttcactac
     5161 ccattttttg atttattttt tcccacgtta ccaatcaagc cttggcttaa aacaattgtt
     5221 accatacatg atgtcattcc cctagtgttt cccaaacaat attctccagg aaaaaagggg
     5281 gctctgatgt taaaaagaca aagagtagca ctcacttttg tttcaactat cattactgat
     5341 tctgatgcat ctaaagaaga tattcatagg tatttgcacg taccactaga gaaaattcaa
     5401 gtcgtgtatc tcgctggtca gcccgagctt caacctgcaa ccgaaattga aattaaacac
     5461 cttaagaagc ggttgggctt aaccaaacca tatgctttgt atgtgggtga tattaactac
     5521 aataaaaata tcccgcagtt aatcaaaatg ctaaagtttt taccaccaaa tattgagctg
     5581 gtgtgttttg gtaaagccat gaagcaaagt gacattccag aatggcaagc aattattact
     5641 caagccgcat taagtaatgt agaaaatcgt gtccatttta ttactgatat tgatagtaat
     5701 gattttcaat ctctttcggc gctgtacggg ggcgcactgt gctatgttca gccttcgctc
     5761 tatgaaggct ttggtttacc cgtactagaa gcgatgcagt gcgccacacc ggttgtatcc
     5821 accaatacat catcgctgcc agaaatagtt ggtaaaaatg gaatacttgt tgacccagaa
     5881 gccgaggcat tagcaaccgg agtcctttct atttatcaac tagcaacttc agatagacaa
     5941 aaaatagttg agaagggact taagcatgct cgtaccttca gttggaataa aactgcacaa
     6001 ttaacggctg aagtttaccg aagggcttta ggaagatgaa acgcttatta tttgttgcaa
     6061 tcattcttat tttatttttt acccgctctt tttggcaaaa tgaaggaatt ccatacactc
     6121 acgatggaga aaatcacctc gcacgttttg ccaatttcaa agtagcgctc aaagaaggtc
     6181 aactgccacc acgttttgcg ccaaatttac tcaatcacta tgggtatccc gtttttaact
     6241 acaactaccc actcgccaat atcatttcgg cgccctttag tgtattaaaa ttccattacg
     6301 aactaacttt taaattgatt attttttttg cactattatt gggaagtttg ggtgtcattc
     6361 gttggcttac tcagtattca agcaaaaaac taccgatttt ggcagcttta atcgcttact
     6421 tatctggacc acctctcatt tcagcccttc tttttcgcgg caatattggt gaaattcttg
     6481 catattcatt gtggccgtgg ttatttttct caatctccga agcgacaaat aaactttcta
     6541 gtaaaagaat tttaatgttt atttttgtgt ggactgcttt tttactctct cacaatatta
     6601 gcgtgtttat tgccgcattt gtttttctca tttatggtgt ttggcaattg ttagtaacaa
     6661 aagagcgtcg gcgatttgcg tcgcaaatcg ccaccatcat aattgcctca atcggtttga
     6721 gtctttggtt ttggctgcct gccattgccg aaatgaaaga agtggttgtt tcgcaggcag
     6781 ccaaccagcg agaatatcta ctccattttc ccacgctcaa acaattactc tatagtccct
     6841 taacgtttgg ttattcttac ccaggacccg tagacaccct gagtttttgg ttaggctgga
     6901 cgatcccatt cagtttatta ctagtatcag caatattctt ggcaaatagt ttttccaaac
     6961 aagataaatt taaaaaaata ctgaattatt ttagatcaca tccgttgggt atatatgtat
     7021 tacttttact acttagtttg tttttacaac tggagatttc tagtactatt tggcagttac
     7081 tacccaattt tagattaatt caatttccct ggcggtttgg catgctttca accatcgtct
     7141 ccctaccagt acttgtttgg ttactgcaaa aaaaccggca actaattact tgcggtattt
     7201 ggttggtttt attcgtacaa ctatttctag tatttcaagc tcatccgatt gattatgtaa
     7261 caaaacaaaa atctgagtat gatcttcacg gaggtacgac ttctacacaa aatgagaatg
     7321 tgccatttac ttttcgatac ttactcatcg ccgattggca accctcgccc tcagtatttt
     7381 ctggagaagc tgaaattacc aaactggagt attggaacgg atcagaccga ctatatcgag
     7441 tcaatgttgt aacaccaaca gtacttgtgg aaccaaccat gtatttcctc ggttgggaaa
     7501 cccagattcg aaatgttgtg actagtagtg attcttttaa aaatgttaca tatatagact
     7561 ctgatgaaat ccaggggaga attgcttatc ggcttgaacc cggaatatat caagtccata
     7621 ctcgatttac tcaaaatacc cctgccagac acattggtaa tgcaagtttt gttatcaccg
     7681 gcatcgcttt atctggatta cttctagcac gtaaataccg gttgtgacac tcaagcaatc
     7741 tctttacaat aactcacata tgaacccaca acctttacgt attattgtca ctggtggatc
     7801 tggattttta ggtcatcatt taattcgtac ctttgttcaa gctggccacc aagtgcgcaa
     7861 cattgatctt cgtcctaacc ctgaatttga aacagcactg gcagatgtgc gtgatcaaaa
     7921 caaaatgaac gaactgatta cagatgcgga cgtggtgttt catcttgctt cactgatcga
     7981 agccggggaa tccgtaaaag aatcggctaa atactttgat aataatgtaa tgggcacttt
     8041 gtcagtgcta gaagcgatgc gacgcaatca aattaaacgc ttcatttttt ctagtagcgc
     8101 agcaatctat ggcgagccaa tgcggacacc tatttcagaa gatgatcgca ctctgccaat
     8161 taatccctat ggtgtgacca agctcgcgat ggaaggattg ctgcgctcat acgtagagtc
     8221 atatagtttc actggcatgg cactacggta tttcaatctc tacggtccag aagaacatca
     8281 cgtaccagaa actcatgcca ttccccgttt tattcaacaa attactcaag acgaagaggt
     8341 aacactttgg ggggatgggg gacacctccg tgattatgtg tatattcacg atattgttga
     8401 tgcccactta ctagccttag actatgccat tgaccatcca aaccagtacc actacttcaa
     8461 tctttctgcc gaacaaccag cttctgtcct agaagtaatc aagcacttag aaaattccct
     8521 caacaaaaaa gccaatatta agcattttcc cgaccgtccc ggtgatcctc gcgtgctgat
     8581 tgccgatgcc accaaagccc atcaacagtt gggttggcat gccgcaacaa ctttatcaga
     8641 aggattaaag aaaaccgttc agtactttac ttctctttga ttaaaacgaa aaaatcgaaa
     8701 cacttagctt ctcagcacac tgtatagttt t
//
LOCUS       DAOQZP010000039         8714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_8549_length_8714_cov_5.788313, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000039 DAOQZP010000000
VERSION     DAOQZP010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 8714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8714
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8549_length_8714_cov_5.788313"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PKH60_03555"
     CDS             complement(<1..352)
                     /locus_tag="PKH60_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17149.1"
                     /translation="MKPLEFSKQIVSKTVRKSWDVATAPTRISLHTMNAFVGLIVDNS
                     EYSNQRKQIAERLGNLHQSLKQSTEKVQYRMAGETEVYSVKMNESNTIILYYRKQKAG
                     YKVSVEQRLMGDYRK"
     gene            complement(339..1325)
                     /locus_tag="PKH60_03560"
     CDS             complement(339..1325)
                     /locus_tag="PKH60_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17150.1"
                     /translation="MSIFRKERGESQTPSPELPFEIDLAYSTILRNVLTSLLAEIQHG
                     LKDTSKKSGVWILKEIERHPRIISEVTSFLIELTPSDIKEEYIATIAQAIIDNKKVVL
                     LGPHWSVADSASFLKVVQLLNKYFKDQGIDRTVTLMGPASSKFFDYRMFASAVIKALK
                     RNGIELLQIAQLQDKELRESIGDEAINKLNRTTWRKILVGMRREGKILGIFQEGTRAK
                     KGRGTAEAPLQILDAIETILDPDSLIVPIMAVGSDLFLGLGDKYPDITHWPKIVMAEP
                     LTLEQARAVSQEINTHPIDVARVAVLANQDEKYWGVLAESITKYRSIHHEAT"
     gene            complement(1340..2299)
                     /locus_tag="PKH60_03565"
     CDS             complement(1340..2299)
                     /locus_tag="PKH60_03565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ17151.1"
                     /translation="MNKFKKSPTVSVIVTVFNEVLTIDELLKSLGDQTLKPKSIIVTD
                     GGSTDGTLKKLHQWQQKKLSVKLEIERVNGNRSQGRNRAVEMATTELIACTDAGCVPE
                     KTWLEELVNEQKSSGALIVAGYYQGLAADNFQVAQVPYLLVMPDKVDSSTFLPATRSM
                     LFNRKWFLENGGFDESLSDNEDYALARKIRDIQAKSGKKILSFTKNAVVAWRPRRTLR
                     EFSWMIFRFARGDARARLWRPKVMLIFLRYFLLLCLVIATLFGRSTTLLIVSLLAASA
                     YMLWSIAKNYRYAQSAWYWLPTLQITSDIAVMSGTIAGLVQKR"
     gene            complement(2292..3980)
                     /locus_tag="PKH60_03570"
     CDS             complement(2292..3980)
                     /locus_tag="PKH60_03570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ17152.1"
                     /translation="MKNSKNSNQSLKVNLGCGSDYRQGWVNVDALKKFKPDIIHDLHS
                     PLPLSDESAEYVLAQDILEHFTKEDLSRLLSDLRRITKNGGLVEIRVPSVTAITNRLR
                     KFPEARNQFLYGTTEFTGIFGAHKVGFTPEYLVFLFISNGFELKTWDLQDTNYHAVFK
                     KINDRVVLTSLTIVAHTFGLGGAEQFITQLAEDYAQRSIQVVVWTNHQPWIAILESKK
                     IAVKKVPIAIDVIGDWKGLLKAILLFPLVLITDGWIVWHAKKSNAILLSGFSQKLSVS
                     LWAALWRRPLIWIEFGPLESVLKKFGQLPKLIYYLAKKLPQIIVVPSNHTFQHLISGA
                     KIDLAKLRAIPCGVAVPKDLSSDSPTIPNRVVCVSRLEPGKGQDRLIVAFKAVVKAMP
                     TAHLRIIGEGGFDQTLRQLIEKNQLTKHVELVGRVPNSLLEMKQAEICVVPSTWELEG
                     FGLTVVEAMMLAKPVIVFDTPPLNELVENEVTGIVIQDDTSPNESQLAKAIISLLSDA
                     QLREKLGISARKQAKRKYSFEKIGASYVQAIEEAMQRMQARVQLKTAGWSRYDE"
     gene            complement(3967..5013)
                     /locus_tag="PKH60_03575"
     CDS             complement(3967..5013)
                     /locus_tag="PKH60_03575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNQ17153.1"
                     /translation="MKILFITPKYIPALGGVETHVQKVSLALQKRGHHITIITAQSSK
                     EMALTEDIDGVCINRIPIEITQQKIATWQWIKANSAIFETADIVHVHDVGWWLLPVFP
                     LIRPKLYITFHGWEGRWPIRWQAKLHRWILSKLSKKTIHIGNWIQEFYWDSPSLVLYG
                     GVDRRNKTIQINQTKHKSRNILKLIFIGRLVKENDIASYCQYFDVLREKQIAFRIHWV
                     GDGPWRKTCEKYGRVLGMVTKPKEFLVRADVVLASSYLSILLARSMGKPVWALYSQRL
                     KQKYLESLPNKEAMVIAGSADELVQETYDEKVKSWKPAAIATQYSKNWPPTWREVATV
                     YETLWQSTTNHEKQ"
     gene            complement(5010..5753)
                     /locus_tag="PKH60_03580"
     CDS             complement(5010..5753)
                     /locus_tag="PKH60_03580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ17154.1"
                     /translation="MLKKIVSFYDHYHLKNNSYSGVIARNNFTYWYILQTLHQPLLKR
                     LKTFTVLDVGCGVGTLALYVAQLCKSVVGIDVSERAIGIAKQAKQFTNSKNLTFKQTL
                     LKQFSNTFDLIICTEVIEHVPNPQQFVELLVKNLHPKGWLLLSTPNRDNWLARIGYYR
                     KFDQEVGHLRRYSVLEITHLLEQNGFKVKYTKTVEGPLRSLLFTSKLGIVIKVIKGPL
                     IPLFHSIDELVGKLFGSSDIVILAQKHTK"
     gene            5752..7566
                     /locus_tag="PKH60_03585"
     CDS             5752..7566
                     /locus_tag="PKH60_03585"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNQ17155.1"
                     /translation="MKIQIGFVLLVVSLIIAVHPQRVTAKTMVFSETFDSPNVFDQWT
                     VVRNRQWQYPLRVCMNLTQPAQWEVVDDTAQIVIDSKPCVMEIVPNNFSIPTLENYLF
                     TIDVTFLESINMDRNLLFLWVDENNWYDIKVTNNEIRLQKVVNGQVYELANAIGSFPF
                     TIDQPYRVEVLFSQQMIRLSIDGALTLTVADSLPFIDAETLKIGLEAGVGSLKRSVSR
                     FDNLIVYLLDDSDLVPIVPHVKQSDPNWAEFEYDHAHEWSNKLTIRNWGCALTSLVMI
                     MQYHGITRMPDGTALTPASLNDWLKLQPDGYIGDGLVNFLAATRLTKQLSILFNTPAL
                     EYGRLNWNSTDRLTPAISEITNHRPVMVQIPGHFMVADGVTADQNDLTIKDPAYNYSL
                     LSQHQVDPLSFRTFLPSHTDLSYLFFVYVPDIHVSIVGPNNESAVEIYEQITAFSDDG
                     ATESTGGLKTTSIAKPIAGQYLVTIQSATNKELEFAAYSYDQYGEVTKHVISKPNLGF
                     GQTFVLDYQTSLPSTIYQVSSFTQFRLLLEKFWYDQQFTTPLAWWWLDKTANTAIHNP
                     AETTYFKRYLANLLGWYEHSMNQEILAELQQELAIIPE"
     gene            7563..8636
                     /locus_tag="PKH60_03590"
     CDS             7563..8636
                     /locus_tag="PKH60_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNQ17156.1"
                     /translation="MKLQSFWHQLTAPAIGLAPMDGITDYPFRSITKKYGRPSVMFTE
                     FTSVEGLGHGAKVLLDDFQFDELQRPIVAQIYGTTPLFFYQTALLLCALGFDGIDINM
                     GCPAKSVQLGGAGAALIKTPELAKTIIRSVKRAVADWQQGFDLFANKEIDPEILLEVQ
                     NRHLQLPTTYQSRARFIPVSIKTRVGYETPVINDWIPTLLEEAPDLITIHGRTLRQGY
                     ANKANWELIAQAADLIHQTDTLVFGNGDVDSYQSAQEHQKLYQVDGVLIGRASFGDPF
                     VFLPPTDESEKPSLAQIALEHAKLYENTYQHHPKYYFAPMRKHLGWYMRGFANAKQVR
                     IELFQSKSPAEVESILRSFNLIE"
BASE COUNT         2495 a         1899 c         1600 g         2720 t
ORIGIN      
        1 ctttgcgata atcacccatc agtcgctgtt ccacacttac tttatatcca gccttctgtt
       61 ttctgtaata taaaatgatt gtattgcttt cattcatttt gactgagtaa acctcagttt
      121 ctcctgccat acgatattga actttttcag tcgattgttt tagagattga tgcaaatttc
      181 ctagtctttc agcgatctgc ttccgctgat ttgaatactc ggaattatca actattaaac
      241 cgacaaaagc attcatggta tgtaggctta ttctcgtggg agcggtcgca acatcccagc
      301 ttttccttac tgtttttgaa acaatttgtt tgctaaattc aagtggcttc atggtggata
      361 cttcggtact ttgttattga ttccgccaag acaccccaat atttctcgtc ttggtttgct
      421 aaaacagcta ctctggcaac atcaattgga tgtgtgttga tttcttgaga tactgctcga
      481 gcttgttcca aggtcagtgg ttctgccatc acaattttcg gccaatgagt tatatcagga
      541 tacttgtctc ctaaacctag gaataaatca cttcccaccg ccattatggg aacaattaaa
      601 gaatctggat caagaattgt ttcgatggca tctaatattt gcaatggcgc ctcagcagtc
      661 ccacgcccct tttttgctct tgttccttct tgaaatattc ctagtatttt tccttcacga
      721 cgcattccaa ccagtatttt tctccaagta gttcgattta atttatttat cgcttcatca
      781 ccaattgact cccttaattc tttgtcctga agctgagcaa tctgcaacaa ttcaatacca
      841 ttacgcttga gtgctttaat cacagctgaa gcaaacatac gataatcaaa aaacttcgat
      901 gaagcaggac ccattaaagt tacagttcta tctatccctt gatctttaaa atacttgttt
      961 aaaagctgaa caactttcag aaaagaagct gagtccgcaa cactccaatg tggaccaagc
     1021 aatactactt tcttattatc aataattgct tgggcaatag tggcaatata ttcttctttt
     1081 atatctgagg gggttaattc aattagaaat gaagtgactt cgctgattat tcttggatgt
     1141 ctttcaattt ctttcagaat ccacacacca cttttttttg aagtgtcttt taacccatgt
     1201 tgaatttctg caagaagcga tgtcaataca tttcttagaa ttgtggaata cgctaggtcg
     1261 atctcaaaag gtaattctgg gcttggtgtt tgtgattcac ccctttcttt tcgaaatatt
     1321 gacataaata gtagattttt taccgctttt ggacaagtcc tgcgattgta ccagacatga
     1381 cagcaatgtc actggtaatt tggagagttg gcagccagta ccaagcagac tgcgcgtatc
     1441 ggtaattttt ggcaatcgac cataacatgt acgcgctggc tgccaataaa ctaacaatta
     1501 atagagttgt tgagcgtcca aacaatgttg caattaccaa gcaaagaagc aaaaaatatc
     1561 tcagaaatat caacattact tttggtcgcc aaaggcgcgc cctcgcatct ccccgtgcaa
     1621 accgaaaaat catccaggaa aattcgcgga gtgtcctgcg tggacgccac gccactaccg
     1681 cattcttcgt gaaactcaag attttttttc cagacttagc ttgaatatca cgtattttcc
     1741 tagccaatgc ataatcttca ttatcactca gactttcatc aaatccccca ttttcaagaa
     1801 accatttacg attaaacaac atcgagcgag tagcaggtaa aaaggtcgac gaatctactt
     1861 tatctggcat cactagtaga tacggaactt gggcaacttg aaaattatcc gctgctaagc
     1921 cttggtaata acccgccaca attagtgctc cactactctt ttgttcatta accaactctt
     1981 ctaaccaagt cttttctggt acacaacccg catcagtaca agcaatcaat tcagtcgttg
     2041 ccatttctac tgctctattt cttccctgac tgcgattgcc atttacccgc tcaatttcta
     2101 acttaactga taattttttt tgctgccatt gatgtaattt cttcaaagta ccgtcggttg
     2161 aaccaccgtc agtaacaata attgattttg gtttgagtgt ttgatcgccg agtgatttaa
     2221 gcaattcgtc aatcgtcaga acttcattga aaactgtcac gatcacagaa acagttggtg
     2281 actttttaaa cttattcatc gtatctactc caaccggcag ttttcaactg aactctcgct
     2341 tgcatacgtt gcattgcttc ctcaattgcc tgaacatacg atgccccaat tttttcaaat
     2401 gaatattttc gtttcgcctg ctttcttgcc gatattccca gtttttctcg taactgagca
     2461 tcgctcagca aactaataat agcttttgca agctgtgact cattgggaga agtatcatct
     2521 tggattacta tgcctgttac ttcattttca accaactcat tgagtggtgg agtatcaaaa
     2581 acaatcacgg gttttgccaa catcattgct tcaactaccg ttagaccaaa accctcgagc
     2641 tcccaggtag acggtactac gcaaatttct gcttgtttca tttctaacag cgaatttggc
     2701 actctaccaa ctaattccac atgtttggtt aattgatttt tttcaatcag ttggcgcaag
     2761 gtttggtcaa aaccaccctc gcctatgatt ctcagatgcg ctgtgggcat tgctttcaca
     2821 acagctttga atgcaacgat caaacgatct tgacctttac ctggttctaa acgagataca
     2881 caaacaactc tgttgggaat agttggcgaa tcactggata agtccttagg caccgctact
     2941 ccacaaggaa tagcgcgtaa cttggctaaa tcaatctttg ccccactaat caaatgctga
     3001 aatgtgtggt tagaaggtac aacaatgatc tggggaagtt tttttgctaa gtaataaatc
     3061 aacttgggca gctgaccaaa ttttttcagt actgattcga gtggtccaaa ttcaatccaa
     3121 ataagggggc ggcgccacag cgccgcccac aatgaaacac ttaacttttg tgaaaatccg
     3181 gacaacaaaa tagcattgga ttttttagca tgccacacta tccatccatc agtaattaga
     3241 actaatggaa ataacaaaat agcttttagc aaacctttcc aatcgccaat tacatctatt
     3301 gcgattggca ctttctttac tgcaattttt tttgattcaa gtatcgctat ccaaggctga
     3361 tggttagtcc aaactacgac ttgaatactt ctctgggcat aatcttcagc cagttgggta
     3421 ataaattgtt ctgctcctcc caaaccaaaa gtgtgagcta ctatcgttaa cgatgttagc
     3481 actactcgat cattaatttt tttaaacact gcatgataat ttgtgtcttg taagtcccaa
     3541 gtttttaatt caaatccatt gctgataaat aaaaaaacta aatactctgg cgtaaatccg
     3601 actttgtgtg cgccaaaaat acccgtaaac tcagtggtac catacaaaaa ttggtttctg
     3661 gcttcaggaa attttcgtag tcgattcgtg attgctgtta cactcggtac cctgatttca
     3721 accaagccac cattcttagt aatccgccta agatctgaga gcaagcgtga taagtcttct
     3781 ttggtgaaat gttcaagtat gtcctgagct aatacgtatt cggctgattc atctgataat
     3841 ggtagtggag agtgtaaatc gtgaatgata tctggcttaa acttttttaa agcatccaca
     3901 ttaacccatc cttgccgata gtcagatccg caacccaaat ttacttttag cgattgattt
     3961 gaatttttac tgtttttcat ggttagtcgt gctctgccaa agtgtttcgt acacagtggc
     4021 cacctccctc caagtcggtg gccagttttt tgaatattga gtggcaatag cggctggttt
     4081 ccaactcttt actttttcat cataagtttc ttgtactaat tcatccgccg aaccagcgat
     4141 taccattgcc tctttgtttg gtaatgattc taaatacttt tgtttcaaac gttgtgaata
     4201 cagtgcccac accggctttc ccatagatct tgcgagtaag atacttaaat aggagctagc
     4261 taaaaccaca tctgctctaa ccagaaactc ctttggtttg gtcaccatac caaggactct
     4321 accgtacttc tcgcaagtct tcctccatgg accatcaccc acccaatgga ttctaaaagc
     4381 gatttgtttt tcgcgtaata catcaaaata ctgacaatat gatgcgatgt cattttcctt
     4441 gaccaatctt cctataaata ttaatttgag tatatttctt gatttgtgtt ttgtttggtt
     4501 tatctgaatt gttttatttc ttcgatccac cccaccatac agcaccaatg aaggagaatc
     4561 ccaatagaat tcctgaatcc aattgcctat gtggatcgtc tttttactta gttttgacaa
     4621 aatccatcga tgtagtttag cttgccaacg aatgggccag cgtccttccc atccatgaaa
     4681 agtaatatat agttttggtc gaattagcgg aaataccggc aataaccacc aacccacatc
     4741 gtgcacatgc acaatgtctg cggtttcaaa tatcgcgctg ttagctttga tccattgcca
     4801 ggttgcaatt ttttgttgtg tgatctctat cggaattctg ttaatacaca caccatctat
     4861 atcttcagtc aaagccattt ccttgcttga ttgagcagta ataatcgtga tgtgatgtcc
     4921 ccgcttttgt aatgctaaac tcactttttg aacatgcgtc tccactccac ccagagcggg
     4981 aatatattta ggtgtgataa acaagatctt cattttgtat gtttctgagc gagaatcact
     5041 atgtcactac taccaaataa tttcccgaca agctcatcaa ttgaatgaaa taacggaatc
     5101 aatggacctt taattacttt tattactatt cccagcttgg aagtgaacag tagtgatctg
     5161 agtggtccct caaccgtttt tgtgtatttc accttaaaac cattttgttc gagtaagtgc
     5221 gtaatttcca ataccgagta acgtcggagg tggccaacct cttgatcaaa tttgcgataa
     5281 tatccaatcc tggctaacca attatcccga tttggagtag aaagtaataa ccaccccttg
     5341 gggtgaagat ttttaacgag taactctaca aattgctgag ggtttggtac gtgctctatc
     5401 acttccgtac aaattatcaa atcaaatgta tttgaaaatt gcttgagtag tgtctgttta
     5461 aacgttagat ttttactgtt tgtaaattgt ttggcttgtt tggcaatacc aattgctctc
     5521 tctgatacat ctatgccaac tactgattta catagttgag ctacatacag tgctagggta
     5581 cctacgccac aacctacatc taaaacggta aatgttttca gtcgtttaag taacggttga
     5641 tgcaaggttt ggaggatata ccagtaggtg aagttgtttc tggctatcac tcctgaatag
     5701 gaattgtttt ttaaatgata gtgatcgtag aaactaacaa ttttttttaa catgaaaata
     5761 cagatcggat ttgtactact agtagttagt cttattatag cggttcatcc ccaacgagta
     5821 actgcgaaaa caatggtgtt ttctgaaaca ttcgatagtc ctaatgtttt tgatcaatgg
     5881 acagttgtaa gaaaccgtca atggcagtat ccactgaggg tttgcatgaa tcttacgcag
     5941 ccagcacagt gggaagtggt cgatgataca gcccaaatcg tgatcgatag caaaccctgt
     6001 gtgatggaaa ttgtgccaaa taatttttca attcccactt tagaaaacta tctgtttacc
     6061 atagatgtta catttttgga atccattaac atggatagaa atttactctt tctttgggtg
     6121 gatgagaata attggtatga catcaaagtc acaaataatg aaattagatt acaaaaggtg
     6181 gtaaatggac aagtttatga actagcaaat gcaattggta gttttccatt taccatcgat
     6241 cagccatacc gcgtcgaagt actgttctca caacaaatga ttcgattatc tattgacggc
     6301 gctctgacac tcacagtcgc tgatagtcta ccatttattg atgcagaaac actgaaaatc
     6361 ggacttgaag ccggagtagg ctctctcaag cgcagtgtgt cccgctttga taacctgata
     6421 gtgtatctac tagatgacag cgacctagtt cccattgttc cacatgttaa gcaaagtgat
     6481 ccaaactggg ctgaatttga gtacgatcac gctcacgaat ggtcaaacaa actcaccata
     6541 cgtaattggg gctgtgcctt gacttcttta gtgatgatca tgcaatacca cggtattacc
     6601 aggatgcccg atggcacagc ccttactcca gcaagtttaa atgactggct aaagttgcaa
     6661 ccagatgggt atataggtga tggtttggtt aattttcttg ccgctaccag attaaccaaa
     6721 cagttaagca tcctttttaa tacaccagcg ctagaatatg gtcggcttaa ttggaatagc
     6781 accgatcggc tcacccccgc aatttcagaa attactaatc atcgaccagt aatggttcaa
     6841 ataccgggtc attttatggt ggccgatggc gtaaccgccg accaaaacga tcttactatc
     6901 aaggaccctg cgtataacta ttcacttttg agtcaacacc aggttgaccc acttagtttt
     6961 cgcacttttt taccaagtca taccgatctc agctatcttt tttttgtcta tgttccggat
     7021 attcacgtat caatcgtagg tccaaacaac gaaagtgccg ttgaaattta cgaacaaatc
     7081 actgctttca gtgatgacgg tgctaccgaa tctacaggtg gattaaaaac aacttcaatt
     7141 gctaaaccca ttgctggtca gtacctagta actattcaat cagccactaa caaagaattg
     7201 gagttcgctg cctattcata cgatcagtat ggtgaggtca ctaaacatgt tatcagcaaa
     7261 cctaatcttg gctttggtca aacatttgtg ctcgattatc aaaccagtct tcctagtacc
     7321 atttatcagg ttagttcatt tacacaattt cgcctactgc tcgaaaaatt ctggtatgac
     7381 caacaattta ctactccatt agcctggtgg tggttagata aaacagcaaa tacagctata
     7441 cacaatcctg cagaaactac ctattttaaa cggtacctag ctaatttact tggatggtat
     7501 gaacatagta tgaatcagga gattcttgct gaattacaac aagaactcgc tataatacct
     7561 gagtgaaact acaatcgttt tggcatcaac taaccgctcc cgctattggc ttagccccga
     7621 tggacggtat tactgattat ccattcagaa gtattactaa aaagtacggt cgacccagcg
     7681 tgatgtttac agaattcacg agcgtagagg ggttgggaca tggagccaaa gttttgttag
     7741 atgattttca atttgacgaa ctgcaaagac cgatagtagc acaaatttat ggcaccactc
     7801 ctttgttttt ttatcaaact gcgctactac tctgcgccct tggttttgac ggtattgaca
     7861 tcaatatggg ctgccccgcc aaaagtgtgc aacttggcgg tgccggggca gccctgatca
     7921 aaacacccga gctagccaaa acaattattc gatcggtcaa acgagccgtg gctgattggc
     7981 aacagggttt tgacttgttt gcaaacaaag aaattgatcc agaaatttta ctggaagttc
     8041 agaaccgtca tctgcaattg cccacaactt accaatcacg agctcgtttt ataccagtaa
     8101 gcataaaaac ccgagttggt tatgaaactc cggtaattaa cgattggata ccaaccctgt
     8161 tagaagaagc gcccgatcta atcaccattc atggccggac acttcgacaa ggttacgcca
     8221 ataaagccaa ctgggaactg attgcgcaag ctgcggatct aatccatcag actgatacct
     8281 tggtgtttgg caacggagat gttgactcat atcaatccgc tcaagaacat caaaagttat
     8341 accaagttga tggcgtgctc attgggcgtg ctagctttgg tgatccattt gtatttttac
     8401 cgccaacaga tgaaagtgaa aaaccatcat tagctcagat tgccctggag cacgccaaac
     8461 tctatgaaaa cacctatcaa catcatccaa aatattattt tgctccaatg cgaaagcatt
     8521 taggttggta tatgcgtggt tttgctaatg ccaaacaagt gagaattgaa ctattccaaa
     8581 gcaagagtcc agcagaagta gagagcattt tacgtagttt taacttgatt gaatgatata
     8641 atttaagttc actccatgga gagtggtcgc atagctcagt tggttagagc gcacgattca
     8701 cattcgtgag gtcc
//
LOCUS       DAOQZP010000040         8419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_8923_length_8419_cov_4.763391, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000040 DAOQZP010000000
VERSION     DAOQZP010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 8419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8419
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8923_length_8419_cov_4.763391"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..707)
                     /locus_tag="PKH60_03595"
     CDS             complement(192..707)
                     /locus_tag="PKH60_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17157.1"
                     /translation="MSKFSVRSIRKQLGSVNPRALYLLTGIIGGLIVVAIFSMIVTKS
                     RTSQTASNQPIESVGLDKLPYWEESNENVLFEWQPVDQEVAIQISGTRRAGNKASSQE
                     TNPGFELVNEGYRSVELNQGKLSPISGISGYQKESEVCVLKTILSAGHEGLLQDNPDV
                     DYELLCGTLPQ"
     gene            complement(759..1802)
                     /locus_tag="PKH60_03600"
     CDS             complement(759..1802)
                     /locus_tag="PKH60_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17158.1"
                     /translation="MTYQDSAFNFKIQKPFFVSDPSTHSMGNALKKYVEIKMHHLTKN
                     QRWKKIHVIGKLDRENSIISDCEKGGKIENWQRPSATFSNQELTILCYPGQHYVQYYA
                     SLISTYLSLTGGDPNTVTYQQPTKEECWEPILRSSLRSIPVSDWLIIGASLTDIGCEG
                     IWQETGDFTFSQQMLGTVKVTYLLCHHTFWGDILSRITRYLVSLGHKRVLFTAKVAGI
                     RPDLIPNYHLATGDTTVLDNRVWQWNNIFKDIKHPQLAFGTHLNSANTIMETKALHRF
                     IKPYDFLDSEIGFFIQGSEEAEERSYLHFISNNLSKEHDENLSNERNPEIIKKRNDLA
                     PIIRSLIQQAINR"
     gene            1848..2414
                     /locus_tag="PKH60_03605"
     CDS             1848..2414
                     /locus_tag="PKH60_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17159.1"
                     /translation="MRVHFMATVTGNQERYKAIVEVIQSHGHTLVTDHYLHRKISEIK
                     QESVAESELYARKVQNWLKQADVVIFEATISDVNVGFELALAMNMSKPVIVMHHKTDG
                     NEPFSLKGIHSDRLQLLPYDEKVLEEMLVIALEYAQETSDVRFNFFITPTISTYLDWV
                     AKEKKIPRSVYLRRLIEREMEENEEYAA"
     gene            2480..3277
                     /locus_tag="PKH60_03610"
     CDS             2480..3277
                     /locus_tag="PKH60_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17160.1"
                     /translation="MSQKLQRQSGQIALIVLLIMGITLVIAVSLAQRTTEDVALTTKQ
                     AETTKVFSAAQSGAEQVLTQVLIALRRGDAVLTGPQAVVGTNDSEVEVTIDEVSEFVQ
                     PLDEGETYQVSLIDPSTTSVTIEWGDANSNCINEASLLISRYNTTGGVTSVNYFGYYP
                     IGSGCSGPGSDGFAPAPSGGTAPRRNAFTYTKQAGDQFLRIMPVYNSTNLFVSGAGAQ
                     QYAITANAQNLLGEDAESRVIQVTTSDLQPPPFLDYAVYSGTTIDKP"
     gene            3302..4306
                     /gene="pilM"
                     /locus_tag="PKH60_03615"
     CDS             3302..4306
                     /gene="pilM"
                     /locus_tag="PKH60_03615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HNQ17161.1"
                     /translation="MPTLALDLGTYSIKVLIGKPGKSAQVTKAFEVINPTGIAHPGDD
                     AQAEQLRQMMTTLWQDYSLPKAQLRLSLPESVVATKVISIPPLSDSELASAIHWQAER
                     HIPIPKEELSLQYQVIARPANKQDATMQVLLVGARKPLVERYLSLFQDIGVEPTLVET
                     QAVSVYRNVGVAESDPVTLIAHLGASELVLQVVTAGRLDFVVSHMGGGTLLTRVLQTT
                     LNLEAGQTEQYVRAYGLDETQFEGKVRDILLPQAREWLREMRIAMQYYANEHTGASVQ
                     RIILSGGVASLRNFAGLVSAELGTEVLVAAPFGPSGVVENVSNPPSFVVAMGLLQYED
                     "
     gene            4316..4912
                     /locus_tag="PKH60_03620"
     CDS             4316..4912
                     /locus_tag="PKH60_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17162.1"
                     /translation="MSFSINFAYHLTKQRTKEQAKDRLLFKKAIIVGVVAAAITTISV
                     IVRVSFDVALSLSEKEEKKIMAQLESLKAQELAYIGYVSKVNVLSELFQNRQAKQQAL
                     KKFRSLFGSGVSIAGLNYAEESNDLEFQLKMSSVFVLERVIGQLDEPQLRVDYKDVVK
                     RGVTRADSGNYTMGVSVGLQVNKPTPTPEKIGSSESSE"
     gene            4915..5700
                     /locus_tag="PKH60_03625"
     CDS             4915..5700
                     /locus_tag="PKH60_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17163.1"
                     /translation="MITQTPNSLLNQVKRRNLFSTSVLIFVVSILLLVIVVYPQVTHI
                     FSTKDKVAAKNKQVALLKAKATQLSQDLIQTEYFTNNDLVQLALPNNKPLLEVLTSLD
                     QVKRAAEIELYDLQTNPGLLATGSGKLGTNKKVVAESMPLKFTVKGTFEQVSQFMDLL
                     EKVAPFTTITSFEIAGSRSTDVVEENSNEPEVIQVSIETKTFFYNPSVKQAVAASLPQ
                     LSQENTVVLAKLAEFMTIPLPEQREITGGGSEDIFGVKTIEGL"
     gene            complement(5702..5956)
                     /locus_tag="PKH60_03630"
     CDS             complement(5702..5956)
                     /locus_tag="PKH60_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004891098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNQ17164.1"
                     /translation="MKNLIEFILIHLVDHPEDVVIDETKGEQETTYLIHVHAEDVGRV
                     IGKGGSVIQSIRQIARVRATKEGERVRIDVFTEDKAEKAE"
     gene            complement(5959..6387)
                     /gene="rpsP"
                     /locus_tag="PKH60_03635"
     CDS             complement(5959..6387)
                     /gene="rpsP"
                     /locus_tag="PKH60_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNQ17165.1"
                     /translation="MLKIKLARFGKKGQPHYRIVVNEAKSKRDGEYVAKLGEYAPTFS
                     PKILNLDLTAYAEWLTKGAQPTETVANLAKRAASKNPFPAKKARVSKKAQAKKLEKIS
                     EKAVEQAPKVTPVEAQPEIQPETQPEVTEETVQPESETKE"
     gene            complement(6404..7090)
                     /gene="rnc"
                     /locus_tag="PKH60_03640"
     CDS             complement(6404..7090)
                     /gene="rnc"
                     /locus_tag="PKH60_03640"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNQ17166.1"
                     /translation="MLPVFQNPQLLTTALTHRSALNEKGSAKKIQSYERFEYLGDAVL
                     ELAVSEFVFHQFPKEPEGVLTSYRSALVKTETLAKISVNLGLGEKLFMSRGEVQTGGR
                     ENKGILADVFEAVVGALYLDQGFPAVVAFLEAVLFPEFQTIMQTKSYQDAKSVLQEKV
                     QSLGFSAPTYRLVEAVGPDHSKQFTIEVLINGVSAATGIGQSKQQAQQQAAQTALEQF
                     NQDLYNPSSS"
     gene            complement(7100..7369)
                     /locus_tag="PKH60_03645"
     CDS             complement(7100..7369)
                     /locus_tag="PKH60_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17167.1"
                     /translation="MANTYHTPQLAEIVIDLIAQHIGSDPISIELIADLEEDLGLLDQ
                     DISQILTLINRQYEELHLTLGELETNEVHTVGDLIQLIDDELAFA"
     gene            complement(7372..7746)
                     /locus_tag="PKH60_03650"
     CDS             complement(7372..7746)
                     /locus_tag="PKH60_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination protein NusB"
                     /protein_id="HNQ17168.1"
                     /translation="MSEHQGNRRVQLLQRLFAFTFTPAQLERGLLEFEESSYFGLLLR
                     NIDQLDQLIAASATERPLSEVNKVDLAILRLLVFESQQKQTPPKVLINEGVEMAKQFG
                     SESSSKFVNGVLGKLLVVESET"
     gene            complement(7751..7915)
                     /locus_tag="PKH60_03655"
     CDS             complement(7751..7915)
                     /locus_tag="PKH60_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17169.1"
                     /translation="MGALPKNKITRAERGKRRRGNTPKLVKDIHTSKTPLHKRGLLAS
                     IVRQSNFTKS"
     gene            complement(7985..>8419)
                     /locus_tag="PKH60_03660"
     CDS             complement(7985..>8419)
                     /locus_tag="PKH60_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNQ17170.1"
                     /translation="KIDVIVSTPIVEVGVDLPQASIMIIEAAERFGLSSLHQLRGRVG
                     RRGQQGYCLVFSGSSDVKAKDRLKIFAKTLDGQKIAELDLANRGAGNLFGVEQHGFDQ
                     LRFASWADTQLIVQAQKIYKKLPKHWQTELFPVQISSSIAAN"
BASE COUNT         2348 a         1724 c         1823 g         2524 t
ORIGIN      
        1 tgtgcatgct gctcccagta gagcggtagt aacagcgcca atccagagtg ccgctctttc
       61 tcgtttatct ctaattctct cactgtttaa aaatgacgcc ataataaatc ctataactta
      121 tatagtagtg attctacaag aaaactgtat catttcaata cacatttgat atatatacta
      181 cacgtttgtg tctattgggg caaggtccca cagagtaatt cgtaatctac atctggatta
      241 tcttggagta aaccttcgtg accagcactt aagatcgttt tgagtacgca cacttcactc
      301 tctttttgat agccagaaat cccactaatt ggactcaact taccttgatt gagttcaaca
      361 ctacgatatc cttcgttcac cagttcaaaa ccaggattgg tttcctggct tgacgctttg
      421 ttacccgccc ttcgggtacc ggaaatttga attgcaactt cttgatcaac aggttgccac
      481 tcaaacaaaa cattttcgtt tgattcttcc caatacggta gtttatctag acctacagat
      541 tcaattggtt ggtttgaggc agtttgtgag gttctgcttt tcgtaactat catgctgaaa
      601 atcgctacaa caatgagacc gccaataatg cctgttagta ggtacagtgc acgagggttg
      661 accgatccaa gttgtttgcg aatagatcga actgagaatt ttgacatacc acaatactac
      721 cgtattttta gaatgttggc tagttctgaa gggattgcct atcggtttat cgcttgttga
      781 atgagtgagc ggatgatggg ggctaagtca tttctttttt taatgatctc ggggtttctt
      841 tcattggaca aattctcgtc atgttcctta ctcaaattgt tggaaataaa atgcaaataa
      901 ctcctctcct ctgcttcctc actaccttga ataaaaaatc caatttcgga atccagaaag
      961 tcgtagggct taatgaagcg gtgtagcgcc ttagtttcca ttatggtgtt ggcggagttt
     1021 aggtgtgtcc caaatgcaag ctgaggatgc tttatgtctt taaaaatgtt gttccattgc
     1081 caaacacgat tatcgagtac ggtggtatcg cctgtggcta gatgatagtt gggaatgagg
     1141 tctggtcgaa tacccgccac ttttgcagta aacaatactc ttttgtggcc aaggctaacc
     1201 aagtatcgcg tgattctgga caatatatct ccccaaaaag tatgatgaca gagcaagtag
     1261 gttactttga ctgtacccaa catttgttgt gaaaaagtaa aatctcctgt ttcttgccaa
     1321 ataccttcac aacctatatc cgtaagactt gcgccaatta ttagccaatc tgagacaggt
     1381 atagatctta agcttgatcg gagaatgggt tcccagcatt cctccttagt aggctgctgg
     1441 taagttacag tattaggatc tccgcccgtg agtgataaat atgtgcttat taacgaagca
     1501 taatattgaa cataatgctg acctgggtaa cacaaaattg ttagttcttg attcgagaaa
     1561 gttgctgatg gtctttgcca gttttcgatt tttccacctt tttcacaatc tgagattatc
     1621 gaattctctc gatcaagctt gccaatcaca tgtatttttt tccaacgctg attttttgta
     1681 aggtgatgca ttttaatttc aacatatttt tttagggcat ttcccatcga atgagtactt
     1741 ggatcagaaa caaagaaagg cttttgtatt ttaaaattaa acgcgctatc ttgatatgtc
     1801 atggtagaat cattatatac agtaagtaag gattaggaaa gataaatatg cgagttcatt
     1861 ttatggcaac tgtaactggt aatcaggaac gatataaagc aattgttgaa gtgattcaaa
     1921 gtcacgggca tacgttagta acagatcatt atctacatcg taaaatcagc gaaattaaac
     1981 aggaatcagt tgctgagtct gaactatatg ccagaaaagt tcaaaattgg ctcaaacaag
     2041 cagatgtagt aatttttgag gctacaattt cggatgtaaa tgtgggcttc gaactcgctc
     2101 tagcaatgaa catgtcaaaa ccagtaattg tgatgcacca caagacagac ggaaatgaac
     2161 cttttagtct taagggaatt catagcgatc gattgcaatt actaccatat gacgaaaaag
     2221 tgcttgaaga aatgcttgtg attgcgcttg agtatgccca agaaaccagc gatgtccgct
     2281 ttaacttctt cattactccc actattagca cctatttgga ttgggtagcc aaagaaaaga
     2341 aaattcctcg ctccgtgtac ctacgtcgtc tcattgaacg agagatggaa gaaaacgaag
     2401 aatacgcagc gtaattaccc tgcatttcca taaaaaccac aacttgcaga gtatccactc
     2461 aatgcggtac tatggtgaca tgtcacaaaa actgcaacga caatctgggc agattgcctt
     2521 aattgttctc ttaatcatgg gtattacttt ggtcattgct gtctctttag cccaacgaac
     2581 aacagaagat gtggctttga caactaaaca ggcagaaacc accaaagttt tttctgcagc
     2641 gcagtctgga gctgaacaag ttttaaccca agtacttatt gcactcagaa gaggcgacgc
     2701 agttttgact ggtccccaag ctgtggtggg cactaatgat tctgaagttg aagtaaccat
     2761 tgatgaggtc agcgagtttg ttcaaccatt ggatgaaggt gagacatatc aggtgagctt
     2821 gatcgaccca tcaactacta gtgtcacgat tgaatggggc gatgccaact ctaattgtat
     2881 aaacgaagca tctctattaa tttctagata taacaccacg ggcggagtaa catcagtgaa
     2941 ttattttggt tattacccca ttggtagtgg gtgctcagga ccaggctctg acgggtttgc
     3001 tcctgcacca tccggtggta ctgccccaag aagaaacgct tttacgtata ccaaacaagc
     3061 cggtgaccaa tttttacgca ttatgcctgt ttataactcc acgaacttgt ttgtgagtgg
     3121 tgcaggagcc cagcagtatg ccattactgc gaacgcacaa aacctcttgg gtgaagatgc
     3181 ggaaagcagg gttattcaag taaccaccag tgacttgcaa ccacctccat ttctggatta
     3241 cgctgtatat agtggtacga caatcgataa accatagtag taaaaaacca acactgccca
     3301 tatgcccaca ctcgctctag atttaggaac atatagtata aaagttctga ttggtaaacc
     3361 tggcaaatct gctcaagtta ctaaggcttt tgaggtaatt aatcctacag gtattgctca
     3421 ccctggggat gacgcacaag cggaacaact gcgtcagatg atgactactt tatggcagga
     3481 ttattcatta ccaaaagccc aactccggtt gtcgttacct gaatcggtgg ttgctaccaa
     3541 agtgatttct attccaccac tctctgattc tgaattagcc tcggcaattc attggcaagc
     3601 agagcgacac attccgattc ccaaagaaga actatctctt cagtatcaag tgattgccag
     3661 acccgccaat aaacaggatg ctactatgca agttctctta gtaggtgcca gaaaaccatt
     3721 ggtggagcgt tatttgtcat tatttcaaga cataggggtt gaaccgactc tcgtcgaaac
     3781 ccaagcggtt tctgtctatc gaaatgttgg tgtggctgaa tcagatcctg ttacattgat
     3841 cgcccacctt ggggcaagcg aattagttct tcaagtggtt acagctggaa ggctagattt
     3901 tgtggtatca cacatgggtg gaggcacgtt actaactcgt gtgttgcaaa caacattgaa
     3961 tttggaggct ggtcaaacag agcaatacgt tcgagcgtat gggttggatg aaacacaatt
     4021 tgaaggcaaa gtgcgagata ttttgttacc ccaggccaga gagtggctga gggaaatgag
     4081 aattgcgatg caatattatg ccaacgaaca caccggagct tctgttcaaa gaataattct
     4141 ttcgggagga gtggcctcgc tccgtaactt tgcaggttta gtgagcgccg aactcgggac
     4201 agaagtactc gtagctgccc catttgggcc ttctggagtt gtagaaaatg tttcaaatcc
     4261 acccagcttt gtggtagcaa tgggactact gcaatacgag gattaatagt gccgaatgtc
     4321 attttcaatt aactttgcct accatctcac caaacaacgc acaaaagaac aagcaaagga
     4381 tcgtttgctg ttcaagaaag ccattattgt tggtgtggtg gctgcggcaa ttacaaccat
     4441 tagtgtgatt gtcagagtta gttttgatgt tgccctctct ctttcggaga aggaagagaa
     4501 gaaaatcatg gctcagcttg agtcattaaa agcgcaggag ctggcgtata ttgggtacgt
     4561 ctcaaaagta aacgtactat ccgaattatt tcaaaatagg caggcgaaac aacaggcgtt
     4621 gaagaaattt cgttcactgt ttggatctgg tgtttcgatt gcggggttaa attatgctga
     4681 agaaagtaat gatctggagt ttcaactcaa aatgagctca gttttcgtat tagaacgagt
     4741 catcgggcaa ctagacgagc ctcagttacg agttgattac aaagatgttg tgaaaagggg
     4801 ggtgacgcgt gcggatagtg gaaactacac aatgggagtt agtgttggtt tgcaggtaaa
     4861 taagccaaca cctactccag aaaagattgg atcaagtgaa agttcggagt aattatgata
     4921 acgcaaacac caaactcact actcaatcaa gtaaaacgca gaaacctatt tagtacttcc
     4981 gtgctgattt ttgttgtctc aatcctccta ctagtgattg tggtgtaccc tcaagtgacg
     5041 catatttttt caaccaaaga taaggtggca gcaaaaaata aacaggtagc actcttaaaa
     5101 gcaaaagcca cccaattaag tcaagatctt attcaaactg aatactttac caacaacgat
     5161 cttgtgcagc tagctttacc aaataataaa ccgcttttag aagtattaac aagtttggat
     5221 caagtgaaac gcgctgcaga aattgaactg tatgatttac aaactaatcc tgggcttttg
     5281 gcaactggca gtggcaagct tggtacaaac aagaaagtgg ttgctgagag tatgccgtta
     5341 aaatttacag ttaagggtac ttttgagcaa gtcagtcagt ttatggattt attggaaaaa
     5401 gttgcgccgt ttaccacaat tacctcattt gaaattgctg gctctcgtag tacagatgtg
     5461 gtagaagaaa attctaatga accagaggta atccaagttt ccattgaaac caagaccttt
     5521 ttctacaatc caagcgtgaa acaggctgtt gctgcttcgc tgccacagtt gagtcaagaa
     5581 aacaccgttg tgttagcaaa actagctgaa tttatgacaa ttcccttgcc ggaacaaagg
     5641 gaaattaccg gtggcggttc tgaggatatc tttggtgtga aaactattga aggtttatag
     5701 attattcggc tttttcagct ttatcttcgg tgaacacatc aatgcgaact cgctcgcctt
     5761 cttttgtggc tctcactcta gcaatttggc ggatggactg gatgacacta ccgcccttac
     5821 caattactct cccaacatct tcggcatgca cgtgaataag ataggttgtt tcttgttcgc
     5881 ctttggtttc atcaattacc acgtcttcgg ggtgatctac taggtgaatt aaaatgaatt
     5941 caattaagtt tttcatagtt attccttagt ttctgattct ggttgtacgg tttcctctgt
     6001 cacttctggt tgggtttcag gttgaatttc tggttgagct tcaactggag tcactttggg
     6061 tgcttgctcg actgcctttt cactgatttt ttcaagtttt tttgcttgag ctttttttga
     6121 aactctggct tttttggcag gaaaaggatt tttacttgca gctcgcttgg cgagattggc
     6181 tacggtctcg gttggttgtg cgcctttggt taaccattcg gcgtatgcag tgagatcaag
     6241 atttaaaatt tttggactaa aagttggagc atactcccca agttttgcta catattcccc
     6301 atcacgtttg cttttggctt cgttgactac aatacgataa tgtggttgcc ccttttttcc
     6361 aaatcgagcg agttttattt ttaacataca gattcctttt gatttaacta gaggacggat
     6421 tatacaagtc ttggttaaac tgctcaagcg cagtttgggc agcctgttgt tgcgcctgtt
     6481 gcttactttg ccctatgcca gttgctgcag aaacaccatt gatgagtacc tcaatagtga
     6541 attgttttga gtgatccggt cccactgctt caaccaatcg gtacgttggg gcggaaaaac
     6601 caagcgactg cactttttct tgcagaacac ttttagcatc ttggtagctt tttgtttgca
     6661 tgatggtttg gaattctgga aagagaactg cttccagaaa agcgacgacg gcaggaaaac
     6721 cctggtctaa atacaacgct ccaactactg cctcgaaaac atcagctaga atacctttgt
     6781 tttctcggcc tccggtttgg acctcgccgc gactcataaa taatttttca cccaaaccta
     6841 gattaacgga gatttttgcc agcgtttctg tttttaccag cgcgctccgg tagctcgtaa
     6901 gcacaccctc tggctctttg ggaaattgat gaaagacaaa ttcgcttacc gctaattcaa
     6961 gcacggcatc acccagatac tcaaaacgtt catatgattg aatttttttg gcgctcccct
     7021 tttcgttgag ggcgctacgg tgcgtgaggg cggtggtcag gagttgagga ttttggaaaa
     7081 ccggtagcat aggtaagagt taggcgaagg ctagttcatc gtcaatcagt tgaattaaat
     7141 ctccaacagt gtgcacctca ttggtttcaa gttcacccaa agtaaggtgt agttcttcgt
     7201 attgtcgatt gattaaagtt agtatttgac ttatgtcttg atccaacaaa ccaaggtctt
     7261 cttccaaatc ggcaataagt tcaattgaga ttggatcact gccaatgtgc tgagcgatta
     7321 aatcaatcac aatctcggct agttgtggag tgtggtacgt atttgccatg tctatgtttc
     7381 agattctact actaatagtt taccaagaac accattaaca aacttggacg aactttccga
     7441 tccaaactgt ttggccattt ctacaccctc attgatgagc acttttggtg gtgtttgttt
     7501 ctgttgtgat tcaaacacaa gtaggcgtag gattgccaga tccaccttat tgacttcact
     7561 cagtgggcgc tcagtggcag aggccgcgat gagctggtca agttgatcaa tatttctgag
     7621 taataaccca aaataacttg attcttcaaa ctctaataat ccgcgttcaa gctgtgccgg
     7681 tgtaaaggta aatgcaaaca atcgctgtaa caattgaact cggcgattac cttggtgttc
     7741 tgacatgcag ttaggattta gtaaaatttg attgtcgaac tatactcgcc agtaagcccc
     7801 gtttgtgcaa cggagttttg gatgtatgaa tatccttaac caactttgga gtgttgcctc
     7861 tacgtcgttt gccacgttcg gctctggtga ttttgttctt aggtaatgct cccataagct
     7921 gttgattgta cctgtcttgt caagtatgcg caaggggttg aaactggcta taaaacactc
     7981 ttagttaatt agcagctatc gagctcgata tttgtactgg aaaaagttcg gtttgccagt
     8041 gcttggggag ttttttatag attttttgag cttggacaat gagctgagta tctgcccagc
     8101 tggcaaaccg aagttgatca aatccgtgtt gttccacacc aaatagattg ccggcgccgc
     8161 gattggccag atctagttct gcaatttttt ggccgtcaag agttttggca aagattttta
     8221 gtcgatcttt ggctttgaca tctgaagagc cagaaaaaac caaacaataa ccctgttgac
     8281 ctcttcgccc cactcttccc ctcaactgat gtaagctaga gaggccgaac cgttctgccg
     8341 cctcaataat cataattgag gcctggggca aatctacgcc cacctcaaca attggtgtgc
     8401 taacaattac gtcaatttt
//
LOCUS       DAOQZP010000041         8305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_9075_length_8305_cov_4.884727, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000041 DAOQZP010000000
VERSION     DAOQZP010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 8305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8305
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9075_length_8305_cov_4.884727"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKH60_03665"
     CDS             <1..311
                     /locus_tag="PKH60_03665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HNQ17171.1"
                     /translation="VIGGIRIQLGNQEFDATISGTLEKVDSLLEVIEVTTADPLSATQ
                     RKLLKDSLQKKLGRPAEMIEIVDASVIAGLRLRVGSKEYDGTFKKRLEKLQATLKSEL
                     "
     gene            308..1873
                     /gene="atpA"
                     /locus_tag="PKH60_03670"
     CDS             308..1873
                     /gene="atpA"
                     /locus_tag="PKH60_03670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNQ17172.1"
                     /translation="MKKATSMPTKPDSFATNLKNLIQQANLTAEPESVGTIVKYGDGV
                     VEVKGLSKAQLGELIDLGKNEFGLALNLKKDVVGIVTLGDARHISSGQTVRRTGRILS
                     VGVSDSIIGRIVDPLGQPIDGKPAIKPEQFMPLEKVAPGVMWRQKVTESVQTGIKAID
                     AMIPIGRGQRELIIGDRSTGKTAIALGTILNQAGQNMICVYVSIGQKRASLAQISQTL
                     TEAGAMDYSIIVAATASDTAAKQYLAPYAGQAIAEYFLGKGKDVLIVFDDLSKHAQAY
                     REVSLLLRRPSGREAYPGDVFYLHSRLLERACRINKEHGGGSITALPIIETQANDVSA
                     YIPTNVISITDGQIYLETDLFNAGMKPAINVGLSVSRVGSDAQKKAMKQVAGTMKLDL
                     AQFRELEAFAQFSSDLDEKTKAQLDRGMRVNSILKQGWDMPLKMAEQVCVIFAAVNGY
                     LDIIALENIQKWEVTFLEYLHSTQTKFLDTITKDAIISDESKAKLQQICKSFSDMHPE
                     LHIPTSKNKDTNG"
     gene            1866..2741
                     /gene="atpG"
                     /locus_tag="PKH60_03675"
     CDS             1866..2741
                     /gene="atpG"
                     /locus_tag="PKH60_03675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNQ17173.1"
                     /translation="MANSRQVKQRINTAKNISKITKAMEMVSASKMKKAQERALSARL
                     YARTMADSLRLLAKATDKASHQLLQENSEGKALGVVIATDRGLCGSLNQNLFKNLIQW
                     KHHHPNGVIVAVGKKAISFCQYYGLEIFAQFSDMPDKITTSDIAPLSSLIVNGFLEST
                     FLSVDLFFMDFINTLSQQPKTEQLLPLRSLSSLDADESLMDSTIHSEYLFEPNPQAIL
                     NELLPFYIENTIYQAFLEARASEHSARMVTMKNASENATELVSDLQLIYNKERQAAIT
                     TELLDITTATASLQQ"
     gene            2750..4144
                     /gene="atpD"
                     /locus_tag="PKH60_03680"
     CDS             2750..4144
                     /gene="atpD"
                     /locus_tag="PKH60_03680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNQ17174.1"
                     /translation="MPNTNYGTITQIIGPVVDVEFTEKLPALLNALHLELADKSIMTL
                     EVEQQLSPTEVRCIALGPTDGLERGLAVSDTGAPITVPVGQVTLGRMFNVVGDIIDEG
                     KPIEVKFTAPIHKSAPLLVDQETRPEMLETGIKVIDLICPFAKGGKIGAFGGAGVGKT
                     VIIQELIRNIAEEHEGHSVFAGVGERTREGNDLYHEMKDSGVLKNTVMCFGQMNEPPG
                     SRLRVALTALTMAEYFRDVKKEDVLFFMDNIFRFSQAGAEVSALLGRIPSAVGYQPNL
                     ATEMGALQERITSTKDGSITAFQAIYVPADDYTDPAPATTFAHIDSTISLDRRLSEQA
                     IYPAVDPLTSSSKILEPAVVGEEHYQVARGVQKVLQKYKELQDIIAILGIDELSEEDK
                     LAVSRARRIQKFLSQPFFVAEQFTNIPGRYVPIAETIRGFKEILEGKHDNLPEQAFYL
                     VGGIDDVIEKAKTI"
     gene            4147..4560
                     /gene="atpC"
                     /locus_tag="PKH60_03685"
     CDS             4147..4560
                     /gene="atpC"
                     /locus_tag="PKH60_03685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNQ17175.1"
                     /translation="MSSKLKLRVVSQEAELLDALVDSVTVPGTEGELTILPYHISLFT
                     KIDVGELIYRSGGQQSSIVVSNGFLNVAPDNNVVVLVDSALHSRDISVQKAEQAIKQA
                     HETMRLSQDRKELLMAEASLRLALLEIKIARKTNQ"
     gene            4619..5104
                     /gene="greA"
                     /locus_tag="PKH60_03690"
     CDS             4619..5104
                     /gene="greA"
                     /locus_tag="PKH60_03690"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNQ17176.1"
                     /translation="MSKTNQTTNQTELTLEGLNELKEELKELEEDKLPAAIERVATAR
                     EHGDLSENSEYHSARDDKELVETRIDQIKSILENAVVVKATKSHQHVGIGSRVTLTKK
                     GSTKKIVYVLVGEYEADPVNGKISVASPLGKALLKKKVGDSAVVSAPAGKTEYTIVEI
                     K"
     gene            5177..6829
                     /gene="lysS"
                     /locus_tag="PKH60_03695"
     CDS             5177..6829
                     /gene="lysS"
                     /locus_tag="PKH60_03695"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNQ17177.1"
                     /translation="MTQPQSPHWIDQVTNSILDWQKETNQTQLHVDDMKTPSGRIHTG
                     SLRGVVLHDLVAKALAEKTQKVVSTYVFNDMDSMDGLPAYLDPKVYTPHMGKPLYQIP
                     VPSLEESGLSLDLISNQDRLELEKATNFAELYAIDFMQAFRRLGCTQEIVWSHQLYES
                     GQMDEIIREALDGVDQLRKIYQDVADYKLPEKWYPFQVICPQCGKVGTTLVTEWDGTE
                     VSFICMPNKVTWAQGCGHEGKISPFGGNGKLLWKVDWPAHWKALGVNVEGAGKDHTSA
                     GGSRDMANAQCPTVFHIQTPFDIPYEWILVRGTKMSSSKGVGTSAREFTNLFPPEIGR
                     FLFASRHYNQVIDFDPYSMAIPDLFDEYDQAARITWDQETGDKRQSRAFQASQIQTQP
                     GPYFLPRFRDIAIWMQHPDLNLVEKFTEVKGSALSQQELQELEDRKTYAKAWVTNYAP
                     SEYQLTPKADVPPEATQLTSEQKTYLKGVLHLLEQDQAWQPDALQQALYETAKSSLGA
                     KLGFQAIYLAFLGKTAGPRAAWMLLAANQDLVRTRLKLLSEE"
     gene            6832..7380
                     /locus_tag="PKH60_03700"
     CDS             6832..7380
                     /locus_tag="PKH60_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNQ17178.1"
                     /translation="MTDQSKLLPPREQSHALLEQYIKNKALRHHCEMVAKAMEAYAKL
                     LNQDVELWYQTGLLHDLDWEQFPDEHPNRALTELLTEYPSEMLQAIAAHAPSRTGKQP
                     ESQLDRYLFACDELSGFLNAYSLMRPEGFTGMKTSSVLKKLRDKSFAANVNREDIQRG
                     FELIEKDPSQHVEFMIGVFDNS"
     gene            7850..>8305
                     /locus_tag="PKH60_03705"
     CDS             7850..>8305
                     /locus_tag="PKH60_03705"
                     /inference="COORDINATES: protein motif:HMM:NF014458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNQ17179.1"
                     /translation="MLDIAYIRQNAKEVKQACKNKQIDSGVVDQLLEVDEKRRSLQQK
                     VDDLRRDSNEHARKLKQNVANGTLPTEADKELGKDFKNKLKELEPQLKEVEDKYQALL
                     YQIPNVPAHDVPIGPDESGNQIIRQVGTAKTFNFEPKTHEELMIQQGWLD"
BASE COUNT         2644 a         1702 c         1778 g         2181 t
ORIGIN      
        1 cagtgattgg tggtattaga attcaattgg gtaatcaaga atttgacgct actatttccg
       61 gaacactaga aaaagtagat tccttgctgg aagtaattga ggttaccact gctgacccac
      121 tatcagccac ccaacgaaaa ttactcaagg attcactcca aaaaaaactt ggaagaccag
      181 ccgaaatgat tgaaattgtt gatgcttccg tgatagccgg actgcgattg cgtgtaggat
      241 caaaagaata tgatggtacg tttaaaaaga gattagaaaa attacaagct acactaaaaa
      301 gtgagttatg aagaaagcaa ccagtatgcc aaccaaacca gattcctttg ccaccaacct
      361 taaaaatctt attcaacaag ccaatttaac ggcagaacct gagtcagttg gtacaattgt
      421 aaaatatggt gatggagtgg tggaggtgaa gggactgagt aaagctcagc tgggtgagtt
      481 aattgattta ggtaaaaatg agtttggctt agcattaaat ttaaagaagg atgtagttgg
      541 aattgttacc ctaggcgacg cccgccatat ttctagtggg cagacagtgc gtcgtactgg
      601 cagaattctt tcagttggcg taagtgattc aattattggc agaatagtcg atccactagg
      661 ccaaccaatt gatggaaaac cagccatcaa gccggagcag tttatgcctc tagaaaaggt
      721 tgctcctggt gtcatgtggc ggcagaaggt gacagaatct gtacaaaccg gtattaaagc
      781 cattgacgct atgattccca ttgggagagg ccaacgcgaa ctgattattg gtgatcgatc
      841 aacgggaaaa actgccattg ccctgggaac catactaaat caagccggac aaaatatgat
      901 ttgtgtatat gtcagcattg gtcaaaagcg tgcttcattg gctcaaatct ctcaaaccct
      961 cactgaagct ggagccatgg attactccat tattgtggct gccactgctt cggacaccgc
     1021 agcaaaacaa tacttagctc cctacgccgg ccaagcgatt gccgaatact ttttgggcaa
     1081 aggtaaagat gttttaattg tctttgatga tctaagcaaa catgcccaag catatcgcga
     1141 agtttctttg ttgctcagaa ggccttctgg tcgtgaagcg tatcctggag atgtatttta
     1201 tctccactct cgcttgctcg aaagagcgtg ccgaatcaac aaagaacatg ggggtggctc
     1261 aattaccgcc ctgcctatta ttgaaacgca agccaatgac gtatcggctt atattccaac
     1321 taatgtcatt tctattactg acggtcaaat ttacctcgaa actgacttat ttaatgcggg
     1381 tatgaaaccc gctataaatg ttggtttatc agtttctcgc gtgggctcag atgctcagaa
     1441 aaaagccatg aaacaagtgg cgggtacgat gaaactagat ttagcacaat tccgcgagct
     1501 tgaagcgttc gctcagttta gtagcgatct agacgaaaaa accaaagctc agctggatag
     1561 aggcatgaga gtcaattcca ttcttaagca gggatgggat atgccattaa aaatggccga
     1621 gcaggtgtgt gtaatttttg ccgccgttaa tggctatcta gatattattg cgcttgaaaa
     1681 cattcagaag tgggaagtga ccttcttgga atatctccat tcaactcaaa caaagttttt
     1741 agataccatt acaaaagacg caattatttc tgacgaatca aaagccaaac tgcaacagat
     1801 ttgcaagtca tttagtgata tgcatcctga attacatatt ccaactagta agaacaaaga
     1861 taccaatggc taattctaga caagttaagc aacgaattaa taccgcaaaa aacatctcta
     1921 agattactaa agctatggag atggtttctg cgagcaagat gaaaaaagcg caggaacggg
     1981 ctttgtctgc cagactgtac gcccgaacta tggctgattc gcttcgtctc ctcgccaaag
     2041 ccacagacaa agcctctcat caattactgc aagaaaattc tgagggaaaa gcactcggag
     2101 tggtaattgc taccgataga ggattgtgtg gcagtttaaa tcaaaattta ttcaaaaatc
     2161 taattcagtg gaaacatcat catcccaacg gcgtgattgt agcggttggt aaaaaagcca
     2221 tctctttttg tcaatattac ggattagaaa tatttgctca attttctgat atgccggata
     2281 aaattactac atctgacatt gcgcccttaa gctcacttat cgtgaacggt tttttggaaa
     2341 gtactttttt atctgttgat ttattcttca tggattttat caatacactt tctcagcaac
     2401 caaaaactga gcaattacta ccactacgct cactcagtag cctagatgcc gacgaatctt
     2461 taatggatag cacaattcat tcagaatatt tatttgaacc aaatcctcaa gcaatactaa
     2521 atgaattgct tcctttttat attgaaaaca ctatttatca agcatttttg gaagccaggg
     2581 cgagcgaaca ttctgcccga atggtaacca tgaaaaatgc gagtgaaaat gccactgaat
     2641 tagtctcaga tttacaactt atatataaca aggagcgtca ggcagcaatt actacagaat
     2701 tgctggacat tacaacagcc accgcttcat tacaacagta aaggaaagta tgccaaacac
     2761 taattatgga actatcactc aaattattgg accagtggtc gatgtagagt ttacagaaaa
     2821 gttaccagct ctgctgaatg cccttcacct cgaactcgct gataaatcaa ttatgacgct
     2881 agaggtagaa caacaattaa gtccaacaga agttcgttgt attgccctcg gtccaacgga
     2941 tggattagag agaggtttgg ctgtttctga tactggagca ccaatcactg taccagttgg
     3001 tcaagtaact ttgggcagaa tgtttaatgt ggtgggagat attattgatg aaggcaagcc
     3061 aattgaagtt aaatttaccg ctcctattca taaatctgcc cctttattag ttgatcaaga
     3121 aaccagacca gaaatgttag agacgggtat taaagtgatt gacttaattt gtccctttgc
     3181 caaaggtggc aaaatcggag cctttggtgg agcgggcgtg ggtaaaacag ttattattca
     3241 agaattaatt cgtaatattg ccgaagaaca cgagggtcac tcggtttttg ctggggtggg
     3301 agaaagaacc agagagggca acgatctcta tcacgaaatg aaagattctg gcgtactaaa
     3361 aaacaccgtg atgtgttttg gccaaatgaa tgagccacct ggaagtcgtt tgcgggtagc
     3421 tctaacagcg cttactatgg ctgagtattt tagagatgta aaaaaagaag atgtgctatt
     3481 ttttatggat aatattttcc gctttagcca ggccggagca gaggtttcag cgctgctggg
     3541 aagaattcct tccgcggtgg gatatcaacc aaacttagca accgaaatgg gcgcactcca
     3601 agaacgaatt acttccacca aggatggttc gattactgca tttcaagcaa tttatgtacc
     3661 agctgatgac tacactgacc cagcaccagc cacaaccttt gctcatatcg attcaaccat
     3721 ttccttggat cgtcgcttat cagaacaagc catttatcct gctgtagatc cactcacatc
     3781 ttcctcaaaa attcttgagc cagcagtcgt tggcgaggaa cattaccaag ttgctcgtgg
     3841 agtacaaaaa gtgcttcaaa aatacaaaga attacaagat attattgcca tattgggtat
     3901 tgatgaatta agtgaagaag ataagttggc tgtctctaga gctagacgca ttcagaaatt
     3961 cctatcacaa ccattctttg tggcagaaca attcaccaac attcctggca gatatgtacc
     4021 gattgcagaa accattcgag gttttaaaga gattcttgaa ggaaaacacg ataatttgcc
     4081 agaacaagct ttttatctgg ttggtggcat cgatgatgta atcgaaaaag caaaaactat
     4141 ctaactatgt cttcaaaatt aaaactcaga gtcgtttcgc aagaagcaga attgcttgat
     4201 gcgctggttg actctgtcac cgttcccggt acagaaggtg agttaactat tttgccctac
     4261 catatttcgt tgtttaccaa aattgatgtg ggcgaactca tctaccgatc tggcgggcaa
     4321 caatcaagca tcgttgtctc caatggcttt ttgaatgtag ctccagataa taacgtcgtt
     4381 gtacttgtgg atagtgcctt gcatagtcgc gatattagcg ttcaaaaagc cgaacaagcc
     4441 attaaacaag cacacgaaac tatgcgtcta agtcaagata gaaaagaatt actgatggca
     4501 gaagcttcat tacgattagc tctgctcgag ataaaaattg ccagaaagac caatcaatag
     4561 tagtatttca ggaccgcgct tgcactttga gtagactttt gtatactaaa gcacttatat
     4621 gtcaaaaacc aaccagacta ctaatcaaac cgaattaaca ctagaaggtt taaatgaact
     4681 caaggaagaa ctcaaagagc ttgaggaaga caaacttcct gcggcaattg aacgagtagc
     4741 aactgctcga gagcacggcg atttatctga aaactctgaa tatcatagcg ccagagacga
     4801 taaggaattg gtggaaacaa gaattgatca aatcaaatca atactcgaaa acgctgtagt
     4861 ggttaaggca acaaaatccc accaacatgt tgggattggt tcacgcgtta ctttgacaaa
     4921 aaaaggtagt acaaaaaaga ttgtctatgt tttagttggt gaatatgagg ccgatccagt
     4981 caatggcaaa atttcggttg cctcaccttt aggcaaggct ttgctcaaga agaaggtggg
     5041 ggatagcgcc gttgtgtctg ctcctgcggg gaaaactgaa tacaccatcg tcgaaatcaa
     5101 ataatcagaa agtttacaaa agtatttctc cccgcacagc gtagctacac agcggggttt
     5161 ttggtaagat gacactatga ctcaaccaca atctcctcat tggatcgatc aagtcaccaa
     5221 ctctattctt gactggcaaa aagaaaccaa ccaaactcaa ctgcatgttg acgacatgaa
     5281 aactccatcg ggcagaattc atacaggttc gcttcgtgga gtggtgctgc atgatttggt
     5341 tgccaaagct ttagcagaaa aaactcaaaa agttgtcagc acctatgtgt ttaacgatat
     5401 ggattcaatg gatggtttgc cagcttattt agatccaaag gtgtataccc cccacatggg
     5461 gaaaccctta tatcaaattc cagtaccctc gctggaagaa agtgggttga gtttggattt
     5521 aatctcaaat caggatcggc ttgaattaga aaaagccacc aactttgccg agctctatgc
     5581 gatagatttt atgcaagcgt ttcgcaggct tggttgtact caagaaatag tttggagtca
     5641 tcaactctat gagtctggtc aaatggatga aattattcgc gaagcacttg atggagttga
     5701 tcaactgaga aaaatttatc aggatgtagc tgattataaa ttgccagaaa aatggtatcc
     5761 attccaagtc atctgcccac aatgtggaaa ggttggcact acactcgtta ctgagtggga
     5821 tggtacggaa gtcagcttta tatgtatgcc caataaagta acctgggctc aaggctgcgg
     5881 ccatgaggga aaaatctcac cttttggcgg caatggaaag ttgctatgga aagttgactg
     5941 gccagcccat tggaaagctc ttggtgttaa tgttgagggc gcgggcaagg atcatacgtc
     6001 tgctggtgga tctagagata tggctaatgc tcaatgccca actgtttttc acatccaaac
     6061 tccatttgat attccctacg agtggattct agtcagagga acaaaaatga gctcaagtaa
     6121 aggagtggga acttcagcgc gcgagtttac caatctattt cctccagaaa ttggccgatt
     6181 tttatttgcc tcgcgccatt acaatcaggt tattgatttt gacccatata gcatggcaat
     6241 tcccgatttg tttgacgaat atgatcaggc ggctagaatt acttgggatc aggaaactgg
     6301 tgataaacgt caatccagag cattccaagc atcgcagata caaactcagc caggaccata
     6361 tttcttgccc cgttttcgcg acattgctat ttggatgcaa cacccagact tgaatcttgt
     6421 agaaaaattc actgaggtga aaggatcggc actctctcag caagaattac aagaacttga
     6481 ggatagaaaa acctatgcaa aagcctgggt taccaactat gccccgtctg aatatcaact
     6541 aacaccaaaa gctgacgtac cgcctgaagc tactcagcta acatcagagc aaaaaacata
     6601 cttaaaaggt gttctccacc tcctagaaca ggatcaagca tggcaacctg acgcgcttca
     6661 gcaagcgctg tacgaaacag ccaaaagtag tcttggagcc aaactgggat ttcaagcaat
     6721 ttatctggct tttcttggta aaaccgctgg accacgggca gcctggatgc tacttgctgc
     6781 caatcaagat ttagtccgta caagacttaa gctactatcc gaggagtaaa tatgaccgac
     6841 caaagtaaac tgcttccccc gcgtgaacaa agccatgcac tgcttgaaca gtatattaaa
     6901 aataaagctt tacgacatca ctgcgagatg gttgcaaaag caatggaagc ctatgcaaaa
     6961 ttgctcaatc aggatgttga actctggtac caaactggac tcttgcatga tctggattgg
     7021 gaacaatttc ctgacgaaca tccaaaccga gcactaacag aattactcac tgaatacccc
     7081 tcagaaatgc ttcaagcaat cgctgctcac gcaccaagca gaacgggcaa gcaaccagaa
     7141 tcacaattag atagatactt atttgcttgt gacgaactat caggttttct caatgcctat
     7201 tcattaatga gaccggaagg ttttactgga atgaaaactt cttctgtact caaaaaactg
     7261 cgagataaat cctttgccgc aaatgttaac agagaagata ttcagcgagg gtttgagtta
     7321 attgaaaaag atccgagcca acacgttgag tttatgattg gtgttttcga taattcatag
     7381 caaatctatt tttatcgcac tcagttcact ccttaaaagc tcgagtggtg ttcaaactca
     7441 gactgcgcca tcatcgattg acgctcaaat atagaactga agcaattatc atgatcaatt
     7501 ccgacaacct tctttttctt caacgcaact acttcccaga ccatagtagt taaagcaaat
     7561 aattggtagt aaagtggttt aataaaagta aagtggttct atggttgcca aaacacattg
     7621 ccgccgcatc gtgtatacta tcgttagtta ataaaagcat ttgaggtggt ttagcagccg
     7681 ccaaagctga ctggggaaat ccagtccggg tacgcccgtg acagcaggaa taagtagcta
     7741 gaggtggcac cttccgccac agcggaacct cttggatgcg ctagttgtgg ttacaattag
     7801 atgcaaacca agaggatttt tttgtactat agagatataa aggagcacta tgctagacat
     7861 cgcttacatc agacaaaatg ctaaagaagt gaaacaagcc tgcaaaaata aacaaatcga
     7921 ctcaggggtt gttgatcaat tgctcgaggt tgatgaaaaa cgacgaagct tgcaacaaaa
     7981 ggttgatgat ttgcgccgag acagcaatga gcatgctcga aaactcaagc aaaatgtggc
     8041 aaacggaaca ttgccaaccg aggccgataa agaactcggc aaagacttta aaaataagtt
     8101 gaaggagctg gaacctcaac tcaaagaagt tgaagacaag tatcaagcac tgttgtatca
     8161 aattcccaat gttcccgccc acgatgtgcc aattggaccc gatgaatctg gaaatcagat
     8221 tattcgccaa gttggaacag caaagacctt caattttgaa ccaaaaactc atgaagagtt
     8281 gatgattcaa caaggttggc tcgat
//
LOCUS       DAOQZP010000042         8165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_9273_length_8165_cov_4.331319, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000042 DAOQZP010000000
VERSION     DAOQZP010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 8165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8165
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9273_length_8165_cov_4.331319"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..1206
                     /locus_tag="PKH60_03710"
     CDS             22..1206
                     /locus_tag="PKH60_03710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNQ17180.1"
                     /translation="MLKNKLRIAFVVVRYGTQVTGGAEFLTRLVAEHLANHISVTVLT
                     TKAIDYVTWKDEILKDNETINGVKILRFSVDYERDLTKFNTKCEEIFNSTNHTFQEEE
                     EWMKIQGPFSSTLLDHIKNNSEEYDLFIFVPYPYATTYYGYQIVKHKAVLLPCAHDEL
                     PLYLDVFKKQFSEARGIIANTPEELELINSVFPVTKRLPSAVIGCGLENVISKSKKRL
                     ASNQKLKPYILYLGRIEAGKGCHELFEYFLNFLTNENSQIELILVGKAIMEIPSHPSI
                     KYLGFVTPEKKYELIKNAICIINPSAYESLSLITMEAWENNKPVLVNGKCDVLRGQTF
                     RAQGGLWYKNFEEFSEMLKWMINNPKKRGIMGNNGKKYVADNYDWPIIEKKYIEFFNS
                     LL"
     gene            complement(1226..2413)
                     /locus_tag="PKH60_03715"
     CDS             complement(1226..2413)
                     /locus_tag="PKH60_03715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNQ17181.1"
                     /translation="MQQHKKVFHKIIYFVHCYPPALGGLEYLSGEIVKLLRDAGHDVQ
                     VITGKGSTLDSYKTFDNWVDETTDPTYIHRLSLRTSAQRLANKFLNKLIFLTGSVSPW
                     YFGPLLEYDKKTLELIQSADVIIGAGMPTMMFYEAYRFAKQYQKRLIVIPAYHDVNYY
                     NRSLPFQNVLSFAHKVLFLSPLEKEQLQKHYRIDSNKLVQFTYCPYTKAEMESAQSKY
                     EKKIVEIEQKLEKGSAITIGFVGQITLRKNFQFFADFIRDNGAELKSKKVRLRFLFAG
                     ARTNSSPQVEAMFKDLDAEIKFIYDFADKNAIYKQIDIFINPSEEESLGLVNFDALAQ
                     GLPVFVHKDSAFASLSNEYKNKILQSNNLTIQTDLFHNFSTLSQFFIYNLVYSSQGLF
                     NYL"
     gene            complement(2400..3128)
                     /locus_tag="PKH60_03720"
     CDS             complement(2400..3128)
                     /locus_tag="PKH60_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012898997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ17182.1"
                     /translation="MSSEASISLHNVSKLFRKQKQKTFKEFLPALFGGQSTSETFWAL
                     SDVNLEIKKGETFGIIGPNGSGKSTLLKLIAGVTKPTKGSITVNGRIAPLIELGAGFH
                     PELTGRENVVLNGVILGMKRVEVEKKFKDIVDFAELWEFIDQPVKHYSSGMYLRLAFA
                     VAVHTDPEILLIDEILAVGDASFQAKCMAKMHEYREQGRTIIIVTHAMETVSSFCERA
                     VFLKQGQVQKLGEAKSVVDAYATA"
     gene            complement(3118..3891)
                     /locus_tag="PKH60_03725"
     CDS             complement(3118..3891)
                     /locus_tag="PKH60_03725"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ17183.1"
                     /translation="MFRTMFKYRELLSELTKREVKQRYKQSILGYAWVILNPFFQMLV
                     MAFVFKFLLRVQDLGVPYAIFLYAGLLPWTLFANSLGSSVNALVGNAGLLTKIYFPRE
                     IFILSTILAKMIDFFLASTVFVAMMIWYKIPITWNVLWFVPIFLIQMLFTYGLSLLVA
                     AFNLFYRDIQYILGLLIMVWMYLTPVIYPTEMFPDRYRFIFQLNPMAVFVNAYRQVIL
                     GGGAPNFISLGIGLLVSVVLTIIGYLVFKKLEGQFADVV"
     gene            4134..4892
                     /locus_tag="PKH60_03730"
     CDS             4134..4892
                     /locus_tag="PKH60_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17184.1"
                     /translation="MPNREIQRFINRAGGALSALALLGLLTSCPEPVPASQANATTPP
                     GTPSHLTIDRYGEQNPETDSIAAIFDDLMKAHLPRLATEYGWTVERSGSILDAKGIQS
                     RFSGVESLVPYHTTSFNGTDYQVYLFPKSEQLKRKGDYAVIPDSPHVVLVKLGQQGSE
                     AYLTDNQKIIDRVLQTIYADIPSLSTREMQVLSTESQLAMRVIQRQWQAKGMGTVRIE
                     DYLFAPDSNWEHDKENGVWLRPLIKAPKTCLTGC"
     gene            complement(4966..5418)
                     /locus_tag="PKH60_03735"
     CDS             complement(4966..5418)
                     /locus_tag="PKH60_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17185.1"
                     /translation="MTKKETNLRRQVEPDVNDEMAPREDKKLIIEKLPKEYLITEFNP
                     IKDFRELELPPIKNEYDLDLRGLMPHLSGRPQMRTLMPTVGEPLRSETFFEDWGGHEH
                     AFPKKPELKPFAITPGHLSAILNALATHGEGEQEIEEAAEEVGETQSS"
     gene            5556..5846
                     /locus_tag="PKH60_03740"
     CDS             5556..5846
                     /locus_tag="PKH60_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNQ17186.1"
                     /translation="MKVPYTFEWDYLKNQFNHFKHHVSFEEAQEAFIDINRLILIDTN
                     HTSKTEKRFYCIGKVKGRICTVRYSKRKGKIRIFGAGYWRKEKKLYEKVNQK"
     gene            5824..6075
                     /locus_tag="PKH60_03745"
     CDS             5824..6075
                     /locus_tag="PKH60_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17187.1"
                     /translation="MKKLIRNKPIVFEGTYGRMRLIKDDLPSPDKLVLRPLSNKITIN
                     LNQKTLRFFKAEADRLGGSYQRMIRNLLDHYAETFQNSK"
     gene            6092..6334
                     /locus_tag="PKH60_03750"
     CDS             6092..6334
                     /locus_tag="PKH60_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17188.1"
                     /translation="MIKKEKLARVVKELPRLLAVALLVSLLAACDSGPTTGGTGNTSG
                     GDTTEPNIIDVNEVLGELGVPTVKPPADGKVPLFAP"
     gene            6355..6510
                     /locus_tag="PKH60_03755"
     CDS             6355..6510
                     /locus_tag="PKH60_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17189.1"
                     /translation="MNRGIEYKKGDEPAGGNDPNEWDPGETDKHYSDKGGHQKRGEEN
                     KKNPKRN"
     gene            complement(6513..6779)
                     /locus_tag="PKH60_03760"
     CDS             complement(6513..6779)
                     /locus_tag="PKH60_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17190.1"
                     /translation="MKNPLEQLANSHNDVANKGCLDWITGELKIIGILAIIAISITAA
                     LIVFGEISSLGFKPLPETDEQSLKTFAQIAFSILTKGGFLGLLA"
     gene            complement(6811..7176)
                     /locus_tag="PKH60_03765"
     CDS             complement(6811..7176)
                     /locus_tag="PKH60_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17191.1"
                     /translation="MPKFKEDIPDKPVEFVKHEASWLKWMVIIAVVGGCVYSMATVGL
                     MGTISDIFTLTGDVLDKASGGSSNNSQTWEPSPNDSGAELAPSPGFDLPDFLKPKNQR
                     PPINCSALQKMNGGFNCIN"
     gene            complement(7262..7990)
                     /locus_tag="PKH60_03770"
     CDS             complement(7262..7990)
                     /locus_tag="PKH60_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNQ17192.1"
                     /translation="MQYNIVILAGGFGTRLKELGETTPKALLHTSQNTLIGELCEAIG
                     LLPNVNQIVLVTNDRFHNQFQFWCQANPKHSLTQIINDGATSPDLRLGALGDLELAAK
                     SLDLHHPTLVLPSDTHFTFSLSELTTLYEQQQSFVTAVYELPKEGIANRLGCASLEEN
                     RVVVFEEKPSEPKSTWACAPFYIYPPNLISRLAEYRLSGGSMDAPSSIIPWLLQQNIP
                     VFAAKVSTAIDVGTQADYERVSQL"
BASE COUNT         2615 a         1591 c         1540 g         2419 t
ORIGIN      
        1 catagaaaat agactgttaa aatgcttaaa aataaattga gaatagcttt tgtagtagtc
       61 cgttatggca ctcaagtaac tgggggggca gaatttctta caagattagt tgcagagcat
      121 ttagcgaatc atatctcagt tactgtgctg acaacaaagg caattgacta tgttacttgg
      181 aaagatgaaa tattaaaaga taacgaaaca attaatggtg taaaaatatt aagattctca
      241 gtagattatg aaagagattt aacaaaattt aatactaagt gtgaagaaat attcaattct
      301 acaaatcaca catttcaaga ggaagaagaa tggatgaaaa tacagggtcc attttcaagt
      361 acacttcttg atcatattaa gaataattcg gaagaatatg acttatttat ttttgtacct
      421 tatccgtacg caactacata ttatggttat caaattgtca aacataaagc tgtattatta
      481 ccatgtgctc acgatgaact gccgctgtat ttagatgtgt ttaaaaaaca attttctgaa
      541 gcaagaggaa taattgcaaa taccccagag gaattagagc tcatcaactc tgtttttcct
      601 gtaactaaaa gattgccttc agctgtaatt ggttgtggtc tagaaaatgt aattagtaaa
      661 tcaaagaaaa gattggcatc aaatcaaaaa ttaaaaccat atatattgta tcttgggaga
      721 atagaagcgg gaaaaggatg ccatgagttg tttgaatatt ttcttaactt tttaacaaat
      781 gaaaattcac aaattgagtt aattctggtt ggcaaggcaa ttatggagat accatctcac
      841 ccctcgataa aatatttagg ttttgtaaca ccagaaaaaa aatatgaatt aattaagaac
      901 gcaatttgca taattaatcc ttctgcttat gaatccttat cactaataac aatggaagct
      961 tgggaaaata ataaaccagt acttgtaaat ggaaaatgtg atgttttaag gggtcaaaca
     1021 tttagagcac aagggggact atggtataag aactttgaag aattttcaga aatgctcaaa
     1081 tggatgatta ataatccaaa aaaaagagga ataatgggaa ataatggaaa aaagtatgtt
     1141 gcagataact acgattggcc tattattgaa aaaaaataca ttgaattttt taactcattg
     1201 ctttaaagaa gtaatttaaa aaatcttata aataattaaa taacccttgt gatgagtaaa
     1261 caagattgta aatgaaaaat tgtgataatg ttgaaaaatt gtgaaataag tcagtttgaa
     1321 ttgttaaatt attagattgt aggattttat ttttgtattc attacttaaa cttgcaaacg
     1381 cactatcctt atgcacaaat accggtaatc cctgtgccag tgcatcaaaa ttgactagcc
     1441 ccaagctttc ttcctctgat ggattgataa aaatatcaat ttgcttataa atagcattct
     1501 tatctgcaaa atcatagata aacttgattt ccgcatccag atctttaaac attgcttcta
     1561 cctgcggaga agaattagtt cgcgcaccag caaatagaaa tctcaatcta acctttttgc
     1621 tctttagttc tgccccatta tcccgaataa aatccgcaaa aaactgaaaa ttcttcctca
     1681 atgtaatctg gcccacaaac ccaatcgtta tcgcacttcc cttctctaac ttctgctcaa
     1741 tttccactat ttttttttca tatttactct gagccgactc catttccgct ttcgtatatg
     1801 gacaataagt aaattgcacc agcttattag aatcaatacg ataatgtttt tgtaattgct
     1861 ctttttctag cggagaaaga aacaacacct tgtgtgcaaa acttagaaca ttttgaaatg
     1921 gcagagagcg attgtaatag ttcacatcat ggtatgctgg tataacaatt agtcgcttct
     1981 gatactgctt ggcaaaccga tacgcttcat aaaacatcat cgtaggcata ccagctccaa
     2041 taatcacatc cgcagactga atcagttcta gtgttttttt atcatactca agtagtggac
     2101 caaaatacca aggggaaact gaacccgtaa gaaaaataag tttatttaga aatttattgg
     2161 ccagccgttg cgccgatgtt cgcagcgata aacgatgaat atatgttgga tctgttgtct
     2221 catcaaccca attatcaaat gttttatagc tgtctagcgt gcttcccttg cctgtgatga
     2281 cttggacatc atggccagca tctcgcagta actttacaat ctctccactt aaatattcta
     2341 aaccacccaa agctggtgga taacaatgga caaaataaat aattttatga aaaacttttt
     2401 tatgctgttg catatgcatc taccacactt tttgcttctc ctagtttttg cacctgtccc
     2461 tgtttcaaaa acactgctcg ctcacaaaaa ctactcaccg tttccattgc atgcgtcacg
     2521 ataattatcg ttctcccttg ttcccgatat tcatgcatct tggccataca cttagcctga
     2581 aatgacgcgt ctccaactgc taaaatttca tcaataagta gtatttctgg atctgtatgt
     2641 accgccacgg caaatgctag tcgcaggtac atcccgctgc tataatgctt aactggttga
     2701 tctataaatt cccacagttc cgcgaaatcc acgatatctt taaatttctt ttccacttca
     2761 acccgtttca ttcccagtat cacgccattt agtactacat tctctctacc cgttaattcc
     2821 ggatgaaaac ctgcaccaag ttcaatgagg ggtgcaatcc gaccattgac cgttatggag
     2881 cccttggtgg gcttggttac ccccgcgatt agcttgagca aggtactttt accactgcca
     2941 ttgggaccaa taatgccaaa cgtttcgccc ttcttaattt ctagattcac atcgctaagc
     3001 gcccaaaaag tttcgctggt agattgacca ccaaataaag ccggtaaaaa ctccttaaag
     3061 gttttttgtt tctgcttgcg aaacagtttg gaaacgttat gaagagaaat ggaggcttca
     3121 gacgacatct gcaaattgtc cttccaattt tttaaagacc aaatagccaa tgatggtcag
     3181 aactacggat actagcaagc caataccaag acttatgaag ttgggcgctc ccccacccaa
     3241 aattacctga cgataggcat tgacgaacac cgccatggga ttaagttgga atataaagcg
     3301 atatcgatca ggaaacattt cggtaggata aatgactggc gtgagataca tccaaaccat
     3361 aattagtaaa cctaaaatat attgaatgtc tctgtaaaat aggttgaagg ctgcgacgag
     3421 taatgataag ccataggtga aaagcatttg aattaagaaa attggcacaa accaaagtac
     3481 attccaagta ataggaatct tataccaaat catcattgcg acgaaaaccg tcgatgcaag
     3541 aaaaaaatca atcattttag ctaaaatagt acttagaatg aatatttctc ttgggaaata
     3601 gatcttggtg agaaggccag cgttgccgac taaagcattg acactgctgc caagcgaatt
     3661 ggcgaaaaga gtccagggta acagcccagc gtagagaaaa attgcatacg gcacacccag
     3721 atcttgtacc ctgagcaaaa acttaaagac aaacgccatg actaacatct gaaaaaaagg
     3781 attgagtatc acccatgcgt aaccaagaat agattgctta tagcgttgtt ttacttcacg
     3841 cttcgttaac tcgctgagca attcacgata cttaaacatt gttctaaaca tgtactagta
     3901 ttgtactgtt gaaattggat cgcgagaagt ggcgctcgtt ttattcgaaa gaggggttaa
     3961 attgagaaaa acacttttct tcacctctat gcaactgtat gccactaagc attttggttt
     4021 tgttggacgt ttgattgtca gtttaatttc ctcctggaac gcctgaatga aaagtgggct
     4081 tttacatagt agctatgata tactatgacc tacaaaagta tattatatac actatgccaa
     4141 acagagaaat tcaaagattt attaaccgag ctggtggcgc actttccgca ctagccttgc
     4201 tcggactact taccagctgt cccgagccag tacccgcttc acaagccaat gccactaccc
     4261 caccaggaac gccatctcat ctcaccatag atcgttacgg cgagcaaaac ccagaaacag
     4321 acagcatcgc tgccatcttc gatgacctca tgaaagccca cttgcccaga ctcgccactg
     4381 aatatggttg gacagttgaa cgatcgggct ctatcttaga tgcaaaaggc attcagtcta
     4441 gatttagtgg tgtcgaatca cttgtacctt atcacactac ttcttttaat ggtacggatt
     4501 atcaagtata tctcttcccc aaatccgaac agctcaagcg caaaggtgac tacgctgtga
     4561 taccagattc cccccatgtc gtgctcgtta aactaggcca acagggctct gaagcgtacc
     4621 tgactgataa tcaaaaaatc attgatcgag tattacaaac catctatgcc gatatcccat
     4681 cactctcaac cagagaaatg caggttctca gcaccgaaag tcaactagcc atgcgagtca
     4741 ttcaacggca atggcaagct aagggtatgg gaaccgtcag aatagaagat tatttgtttg
     4801 ctccggattc aaattgggaa cacgataaag agaatggcgt ctggctcaga ccactcatca
     4861 aagcaccaaa aacctgctta actggttgct aaatagcatc tcggaataac tattctcatc
     4921 gcttgatttt ctactccttg tgtctgtttc ttgagttatt gtgaatcaag aactctgagt
     4981 ttctccaacc tcttctgctg cttcctcaat ttcttgttcg ccttctccgt gagtcgctaa
     5041 agcgtttagt attgcagata aatgccctgg tgttattgca aatggtttta gctctggttt
     5101 tttagggaat gcatgttcat gtcctcccca gtcttcaaaa aatgtctcac ttcttagcgg
     5161 ttcaccaaca gttggcatta gtgtacgcat ttgaggtctg ccactaaggt ggggcataag
     5221 acctcgtaga tctaagtcat actcattttt gattggaggt aactccagct cacgaaaatc
     5281 ctttatcgga ttaaactcag ttattaaata ttctttaggt aatttttcaa ttatcaactt
     5341 cttatcctct ctgggtgcca tttcatcgtt cacgtctggt tcgacttgtc tacgtaggtt
     5401 tgtttctttt tttgtcatag tacctcagca ttataagtct tacataggat aaatcaagtt
     5461 tagggcttag atgaatactt tttcaataca gttgatgtaa gtattaaggt ttagagtagt
     5521 gctctaaagc aggtgaatat tgacattata tacatatgaa agtaccatat acatttgagt
     5581 gggattattt aaaaaatcaa tttaatcatt ttaagcatca tgtttctttt gaagaagctc
     5641 aagaagcctt tatagatata aacagattaa ttttaattga tactaatcac acatcaaaaa
     5701 ctgaaaaacg tttttattgt ataggaaaag ttaaaggaag aatctgtaca gttagataca
     5761 gtaaaagaaa aggaaaaatt cgaatatttg gagccggata ttggcgaaag gaaaagaaat
     5821 tgtatgaaaa agttaatcag aaataaacca attgtatttg agggaaccta tggtcgcatg
     5881 cgactgatta aggatgattt accctcacca gataaactag tccttcgacc tctgtctaat
     5941 aaaatcacaa ttaatttgaa ccaaaaaaca ctgcgctttt ttaaagctga ggctgacaga
     6001 ttgggagggt cctaccaacg gatgattcgc aacttgctgg atcattacgc agaaacgttt
     6061 caaaactcaa agtgatatac tatcaattat tatgatcaag aaagagaaac ttgctcgggt
     6121 tgtaaaagaa ttaccccgtt tgttggcggt agcgttatta gtatcgctat tggctgcttg
     6181 cgatagtgga cctaccactg gtggaactgg taatacaagc ggtggtgaca caactgagcc
     6241 taatattata gatgtcaatg aggttttagg tgaacttggc gtgccaacgg taaaacctcc
     6301 agcagatgga aaagtgccac tatttgcacc gtaatagtta aacgaaagta gcgtatgaat
     6361 cgaggtatag aatataaaaa aggtgatgaa ccggcgggcg gcaatgatcc taacgagtgg
     6421 gatccaggtg aaactgataa acactactca gataagggtg ggcatcaaaa acgaggtgag
     6481 gaaaacaaaa aaaatccaaa acgaaactaa gactacgcaa gtaggcctaa gaagccacct
     6541 ttagtaagaa tagagaatgc aatttgcgca aacgttttca gggattgttc gtcagtttct
     6601 ggtagtggtt taaatcctaa tgaactgatt tcaccaaaaa cgattagcgc cgcagttatg
     6661 cttatagcaa taatcgcaag aatgccaatt attttcagct caccggtgat ccaatcaaga
     6721 catcctttgt ttgcaacatc attgtgagaa tttgccaatt gttctaatgg atttttcata
     6781 atgtaagaga tgctaaacaa aaattagcaa tcaattaatg caattgaacc caccattcat
     6841 tttttggagg gcgctgcaat ttattggtgg tcgttgattt tttggtttga gaaaatctgg
     6901 tagatcgaaa ccaggggatg gtgccaattc agcaccagaa tcgtttggtg aaggttccca
     6961 tgtctggcta ttgtttgaac ttccaccact cgctttatct aaaacatcgc cagtgagtgt
     7021 gaaaatgtca gaaattgtac ccatgagacc taccgttgcc attgaataga cgcatccgcc
     7081 aacaacagca ataattacca tccatttaag ccagctagcc tcatgtttta caaactcaac
     7141 aggtttgtct ggaatatcct ctttaaattt cggcactaga tcatactata ccacaaaaat
     7201 acgaatctca cccgcttggc ttgatgcact agctagaatt gcctagcaag atttcacttt
     7261 ttcaaagctg actaactctt tcgtaatctg cctgggttcc aacatcaata gccgtgctga
     7321 cttttgcggc aaaaactggt atgttttgtt ggagcaacca aggaataatt gaacttggcg
     7381 cgtccatact cccgccggat aaacgatact ctgctagtcg agaaattaga ttcggtggat
     7441 agatataaaa tggcgcgcat gcccacgtgc tttttggttc tgaaggtttt tcttcaaaaa
     7501 cgactactct attttcttct aaactggcgc agccaagccg attagcaata ccttctttgg
     7561 gtaactcata aacagccgtc acaaaagatt gctgttgttc atacaacgtg gttaattcac
     7621 tcaatgaaaa tgtgaagtga gtatcgctgg gcaaaactag tgtggggtga tgtagatcaa
     7681 gacttttggc agcaagttca agatctccca gtgctccaag acgtaaatct ggacttgtcg
     7741 ctccatcatt aataatttga gtcaacgaat gtttgggatt ggcttggcac caaaattgaa
     7801 attgattgtg gaaacggtca ttggtgacca aaacgatttg attgacgttt ggaagtaacc
     7861 caattgcttc acataactca ccaatgagag tgttttggct agtatgcaac aaagcctttg
     7921 gtgtggtttc acccaactct tttagtctag taccaaatcc cccagcaaga atcacaatgt
     7981 tgtattgcat acgagtaatt gtaacaaaat agtctaaggt gcggtatgat agttttgatg
     8041 aaaaaaagcg ctgcagtggc atttggcgta gtgattggta tttttttatt ctttcggttg
     8101 tttaacctac aacagacgtt tgtgctattt ggtgactctg cgcgagattt gttggagtta
     8161 catga
//
LOCUS       DAOQZP010000043         8097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_9365_length_8097_cov_4.969162, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000043 DAOQZP010000000
VERSION     DAOQZP010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 8097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8097
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9365_length_8097_cov_4.969162"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PKH60_03775"
     CDS             <1..601
                     /locus_tag="PKH60_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17193.1"
                     /translation="TFAGMGRMASFIEEFQTVAAEHIPKLVEDEKFPDLMVSCDTKND
                     QLMKFLTRDNQFKRRSDQSGRELGRFIDSELKAIISLTRIHEIQNKLEKLFNEKRAEL
                     QQPTATSDVAPNWTESSTTPNLGGWVTNKTTVAQEPVASDQTNTAGGWLSPDTTQSGQ
                     QVTYEAQLDAQKPGDDAAVEDNLDELIAVSQAQNKQHNN"
     gene            640..1935
                     /locus_tag="PKH60_03780"
     CDS             640..1935
                     /locus_tag="PKH60_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17194.1"
                     /translation="MHLISRVSKSAFFRGSAIFTITSFAVSVLNYLFNLIVARYFSLE
                     VYGEYSTTFSYLAVIMVPLGAFGLVLIKRIGATAVADRFGFILAVEAWVIRLLTKVWL
                     PILGLIAMGVLLLLRFSNLAMPSIVMIVFSGLIAIFTTMYTAGLQASKRFFASGAIPA
                     TGSALKILLVLVVILLVPTLENLYLALIIDGVITIAIYRWYLKRFRPELRYHLPSISV
                     LARYIKSKTLTVPLFATLGMVGMLSVDVMLVKKFFAPADVGLYAGLALLGRIILYVTG
                     PIAAVAFAFFTGHESKQQRGKILLLTTLLFMAIGLVATLGYLLTPNLVVGIIFGQRFI
                     SVASIIYVAAIFGTMYSLANLFAQYYISREEPIAMVSIVFLAIQVVGITMFHENFTQV
                     LIVGTVASSGLFLSYLLFYVWNNQRVLGRLLQGSFKKAN"
     gene            2070..2420
                     /locus_tag="PKH60_03785"
     CDS             2070..2420
                     /locus_tag="PKH60_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17195.1"
                     /translation="MSLFEPGPTHLDPQQLLDLEKRKQLLDHCLEVIQQPQFRELILS
                     EIALALASNQSEIHVSLPVVGQPSETQAMFSYFDDSPPIDLIIENGQAYTLRLMFMKV
                     GATTIDCDLLIVNY"
     gene            complement(2533..2781)
                     /locus_tag="PKH60_03790"
     CDS             complement(2533..2781)
                     /locus_tag="PKH60_03790"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNQ17196.1"
                     /translation="MQVVISHQAKKQFSKIPSVNQPKVKSRLELLAQSPLAGKPLKGQ
                     LEGCYSLRCWPYRIIYQIDVKQHQIVVLAIIHRQGAYR"
     gene            complement(2787..3044)
                     /locus_tag="PKH60_03795"
     CDS             complement(2787..3044)
                     /locus_tag="PKH60_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNQ17197.1"
                     /translation="MSKTTILTPSQARADLYNVINQASAGTTEFLIQSKNGKTAVILS
                     QDELESWRETAEILMTPGAQESILAGKKEVLKGKVIPISEI"
     gene            complement(3153..3341)
                     /locus_tag="PKH60_03800"
     CDS             complement(3153..3341)
                     /locus_tag="PKH60_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17198.1"
                     /translation="MSFSLEGTGIPGPHMMRENKFPEFSAYIAVEPVIESTEATVLPP
                     REQVYADVDPKPEIVKAE"
     gene            3457..4062
                     /locus_tag="PKH60_03805"
     CDS             3457..4062
                     /locus_tag="PKH60_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17199.1"
                     /translation="MAGQQIEAIRSKSMGEQYAAREMDRAFETFAQLIEIALISVRDT
                     TSISLTPNKTREEIMQLINFDVAMKVVPLYIETIIKIMRSASTNSVSELLISLDTTLR
                     YFHRQIESLLTYFYQGTTSVDAMGYKVYKSPISASSEQITSFREVLIGSLVAVTVIST
                     LTQRVNPFAYSAQNQYRDVLAQCNKCQAEWTRILANTRITA"
     gene            complement(4070..4357)
                     /locus_tag="PKH60_03810"
     CDS             complement(4070..4357)
                     /locus_tag="PKH60_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006723909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNQ17200.1"
                     /translation="MKKYKTRPYEEMRAELMADPAFKKAWDARANQRRVTRNLIEARI
                     KHKLTQRQLAERIGIKQPSLARIERGTHLPSIATLEKIAAGLGKKLEIRFV"
     gene            complement(4354..4683)
                     /locus_tag="PKH60_03815"
     CDS             complement(4354..4683)
                     /locus_tag="PKH60_03815"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNQ17201.1"
                     /translation="MISIKTITSASGKDLIEEFITSLKGADRQECIDTIDLLSEYGFM
                     LSSKYIKKLTNKPKLWELRVSGKNEYRLLFTYKNQVATIVHAFTKKTTKTPQKEIALA
                     SNRLKRI"
     gene            4800..5717
                     /locus_tag="PKH60_03820"
     CDS             4800..5717
                     /locus_tag="PKH60_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ17202.1"
                     /translation="MKLIVTIPAYNEVSTISQVIASIPRKLPGIVSVKVLVWSDGSTD
                     ETVAKAKTAGADYVFANKKNLGLARTFDLATKKAVELGADIVVNTDADNQYDQSEIAM
                     LVAPLIAETADVVNGNRQVESLTHMSHSKKYGNQLGSWVIRWLTGLKIQDASSGFRAY
                     TAEAIRKLTIFSKHTYTHETLIQIAFGDLQITEVPVTFNARQSEAGPSRLVSNVFTHI
                     TKSGSTIIRTILTYKALRVFITMGALELGLAVLIGLRYLWFMFNGLSGGHVQSLILAS
                     MLFNMGLVSILIGIIADLIAVNRKTHESI"
     gene            5704..5979
                     /locus_tag="PKH60_03825"
     CDS             5704..5979
                     /locus_tag="PKH60_03825"
                     /inference="COORDINATES: protein motif:HMM:NF019214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HNQ17203.1"
                     /translation="MNQFKFDIWTLWGLTAQGFYFARFVLQWVQSERQGKIVIPHVFW
                     ILSLIGAVMIIVYSLVRSDLVLLITGLLQLVLFSRSLILSKKLKKTT"
     gene            5981..6607
                     /locus_tag="PKH60_03830"
     CDS             5981..6607
                     /locus_tag="PKH60_03830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ17204.1"
                     /translation="MQKKPFVEGNLEDKYNAKNPVSKYLMQRFLRSFEQLLVEVKKQE
                     RITTICEVGCGEGELLKILHTHFPKAKLYACDLASNEIAKAKCNTQELKVTFTQQNAE
                     SLHIYADQQFDLVVCCEVLEHLEQPKKGLAELKRIGRTAVVSVPVEPLWRFLNLLRFK
                     YVKHLGNTPGHLNHWSTDSFQNLLRKRSLKVLQSKFPLPWQMYLFEAK"
     gene            6611..>8097
                     /locus_tag="PKH60_03835"
     CDS             6611..>8097
                     /locus_tag="PKH60_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17205.1"
                     /translation="MFVPWIFGDEYLYLSKARNIQRGIDVLADVSQGHTYPPLYSYLL
                     SLVMGTDPLISYQHAQWLNVGVSQLLIILALLMLNRVFRWTDSKKGWVYLLLLYLAVA
                     TSTMITGFYFVAMSENLYTPLVVLIFSLVVYISKSTKLLTKQTWLLVGTVGILCGLAV
                     LTRTIGYVLLPSVMASLLFVGMPNKIQHKYKILIALAVGLIAIGAIQTFNLWEASQVV
                     RTELQQTHYEELSSGYMGVFQAFLTGTGNWFMALKILGNHLTYLLFASFFFPLLFLMN
                     DVVKMLKTKKIDAMTVFVSLFGLGSLAFSFLHCYFGFQTEPIKYSTYFRYFDQTILIF
                     ILYGLIRLWQWLQNKEKFNKLAVLLFAVVSLVGLVFLPPRDFYMTLNAFGWAWLDLFI
                     NNQWMIRVVGFALIVLSSMLVWKKQLYPLLLVVIIGFQVASIPTISKMHEWLSHDFKA
                     LVEPVRRVAVDQGITNWYITSDYQEKGMLGDLYFIKYLLLFYGNE"
BASE COUNT         2233 a         1554 c         1748 g         2562 t
ORIGIN      
        1 aacgtttgct ggtatggggc gtatggcctc gtttatagag gagtttcaaa cagtagccgc
       61 cgaacacatc ccaaaattgg ttgaggatga aaaatttcca gatcttatgg tcagttgtga
      121 tacaaaaaat gaccaattaa tgaagttttt aaccagagac aatcaattta aaagacgatc
      181 cgatcaaagt ggcagggaac ttgggaggtt tattgactcg gaactcaagg caattatttc
      241 gttaacacga attcatgaga tccaaaataa acttgaaaaa ctgtttaatg aaaagcgcgc
      301 tgaattgcaa caaccaacag ctacgtcaga tgtggcgcca aactggacag aatcgtctac
      361 caccccaaat ttgggtggtt gggtaactaa taaaacaacg gtggctcaag aacctgtggc
      421 tagtgatcaa acaaatactg ctggtggctg gctatcacct gatactactc aatctggtca
      481 acaggttact tacgaggctc aacttgatgc tcaaaaacct ggtgatgacg ccgcagtaga
      541 agataatttg gacgaactaa ttgcggtatc tcaagcccaa aataaacaac ataacaatta
      601 agtcgtattt tgtaccgact ttacagtaca attactacga tgcatttgat ttcacgagta
      661 tctaagagcg cgttttttcg tggttcggct atctttacca ttacttcgtt tgcagtgagt
      721 gttttgaact atctgttcaa cttaatcgtt gcccgctatt tctctttgga agtatatggc
      781 gaatactcaa caacattttc ctatcttgct gtgattatgg tgccactcgg tgcgtttggt
      841 ttagtgctta ttaaacgaat tggggcaacc gcggttgcag atcgatttgg ttttatctta
      901 gctgttgagg cctgggtaat ccggttatta acaaaagtat ggcttccaat actaggcttg
      961 attgcgatgg gagtgctttt attacttcgg ttttcaaatt tggcaatgcc tagtatcgtt
     1021 atgattgttt tctctggctt gattgcgatc tttacgacaa tgtatacagc tggattacaa
     1081 gccagcaagc gcttttttgc ctctggagcg atacctgcaa ctggatcggc actaaaaatt
     1141 cttttagtct tggtcgttat cttgctagtg ccaaccttgg aaaatttgta tcttgctctc
     1201 attattgatg gagtcattac cattgcaatc taccgttggt atctcaagcg ctttcggcct
     1261 gaactgcgat atcacctacc ctcaattagt gttttagcaa ggtatataaa aagtaaaaca
     1321 ctgacggtcc cgctatttgc tactttgggt atggttggga tgttgagtgt ggatgtgatg
     1381 ctggtaaaaa aattctttgc cccggctgat gtaggtctct atgccggatt ggccttgtta
     1441 ggtagaatca tactatatgt cacgggcccc atcgcagcag tcgcttttgc gttttttact
     1501 ggtcatgaga gcaaacaaca gcgcggaaaa atcttattgc tcaccacttt gctattcatg
     1561 gcaattggat tggtagcgac gctcggatat ttgttaaccc caaacctggt agtaggaatt
     1621 atttttggtc agcgatttat ttctgttgcc agcatcatct atgtagctgc catctttggc
     1681 acaatgtact ctctggcaaa tttatttgct caatattata tttcgcgtga ggaaccaatt
     1741 gcgatggtct caatagtatt cttagcaatt caggttgtgg gcattacgat gtttcatgag
     1801 aatttcacac aggttttgat agtaggaact gtagcctcca gtgggttatt tctgagctat
     1861 ctgttgttct atgtttggaa caaccagcga gttttgggca gattgttgca aggatccttc
     1921 aagaaagcga actaaaaccc caaaactgat ccaccaagct ttttttaaca aataggtgac
     1981 tgttgggatt gacagtaata acctctagta tagaatacta catattgact atgtatcagt
     2041 aactgtctta gcgtagtgaa aggtactaca tgtcactatt tgaaccaggt ccaacccatc
     2101 ttgatccaca gcaattatta gatttggaga aaaggaagca attgttggat cactgtttgg
     2161 aggtcattca acaacctcaa tttcgagaac tcattctttc agaaatagct ctggcactag
     2221 catccaatca aagtgaaatt cacgtttctt tgccagtggt tggccagccc tcagaaactc
     2281 aagctatgtt tagttatttt gatgatagtc ctcccattga tttgataatt gaaaacggtc
     2341 aagcatatac tttacgactg atgttcatga aagttggtgc gaccacaatc gattgtgatc
     2401 tcttgatagt gaattactaa cagaagaacc catcaaaata ctctagggca tttggagcag
     2461 actttcagat cctctagcta gacaaatagc atcgaaaggt cgctctgagc taacacagag
     2521 aggcagcgta cgctatctgt atgctccttg acggtgaatg atcgccagca ccacgatttg
     2581 atgttgtttt acatctattt ggtagatgat cctgtaaggc cagcagcgaa gtgaataaca
     2641 tccctcaagt tgtcctttta acggttttcc tgccagtgga gattgagcga gtaattccaa
     2701 tcggctctta acctttggtt ggtttactga ggggatttta gaaaactgtt ttttagcttg
     2761 atgagagata acaacttgca taccgatcaa atttcggaaa ttggtataac tttacctttt
     2821 aacacttctt ttttacctgc gagaatgctt tcttgagctc ctggcgtcat gagtatttca
     2881 gcagtttctc tccaagattc aagctcatct tgactgagta tcactgcagt tttaccgttt
     2941 tttgattgaa tcagaaactc agtagtgcca gcgctggctt gattgatgac gttgtataaa
     3001 tcagctcggg cttgcgatgg agttagaatg gtagtttttg acatactgaa atcgtacgac
     3061 atagcgtacg taatgtcaat tgttgactta gctggttgaa gtatcacgaa ccaaacgata
     3121 cgactagaat cttacaaaaa gcgaaacaag tgttattctg ctttgacaat ttctggttta
     3181 ggatcaacat cggcataaac ctgttctcta ggaggtaaga ctgtagcctc ggtcgactca
     3241 atcactggtt ctacggctat atatgcggaa aattctggga acttattctc tctcatcatg
     3301 tgtggaccag gtatacctgt tccttcaagt gaaaatgaca tagcatgctt tcttctaata
     3361 catactaaat atgtattata ctcccactca aattatcatg taattatttg caatcctgat
     3421 gtacaatgat gcatataaat atagatataa atgtatatgg ccggtcaaca aatagaagcg
     3481 attaggtcca aatctatggg tgagcaatat gctgcccgtg aaatggatcg ggcttttgaa
     3541 acatttgcac aactaataga aattgcacta atctccgtga gggatactac aagtatttct
     3601 ctaaccccta ataaaactag agaagaaatt atgcaactta tcaattttga tgtcgctatg
     3661 aaagttgtac cgctctatat agagacaata ataaaaatta tgaggtctgc aagtactaat
     3721 tccgtatcag aactattgat tagtcttgat acaacattga gatattttca tcgtcaaatt
     3781 gaatcgctcc taacgtattt ctatcagggt actacgagtg tagatgcaat gggttataag
     3841 gtttacaaat cacctatctc agcatctagt gagcaaataa ctagtttcag agaagtactt
     3901 attggttcgt tagttgcagt cactgttatt tcaacactca ctcaaagggt taatccattt
     3961 gcatattctg cacagaatca atacagagat gtgttagcgc agtgtaataa atgccaagca
     4021 gaatggacta ggattttggc taatacgaga attaccgctt aatttttagt tagacgaatc
     4081 taatttcaag tttttttcca agacctgcag cgattttttc taaggtagcg atggaaggca
     4141 ggtgggtgcc tcgttctatt cgagccaaag aaggctgttt gatgccaatc cgttctgcca
     4201 gctggcgttg ggtgagcttg tgtttgatgc gggcttcgat gagattgcgg gtaacccggc
     4261 gctgatttgc tcgagcatcc caggcttttt tgaaagctgg atctgccatg agttctgctc
     4321 tcatttcttc atatggtcga gttttgtatt ttttcatatt cgtttaagcc tgttgctagc
     4381 tagagctatt tctttctgag gagttttggt tgttttttta gtaaacgcat gaacaatcgt
     4441 cgctacttga tttttatatg tgaatagcaa tcgatattca tttttaccac tcactctcag
     4501 ttcccaaagt ttcggcttat tagtaagctt ttttatgtat ttactggata gcataaagcc
     4561 gtattcacta agaaggtcga tggtatcaat gcattcttga cggtccgcac cctttagtga
     4621 tgtaataaac tcctcaatta gatcttttcc actagcactg gtaatggttt taatactaat
     4681 catataagat aacattaatg ttattaacaa acaatcattg attttttacg tatgcagcga
     4741 agcctaactc accatgcttt cgcgaagtgt agtgggttgt tttgtagtaa aatgattgaa
     4801 tgaaattgat tgtgacgatt cccgcgtata acgaagtgtc gacgatcagc caggtgattg
     4861 catctatccc ccgcaaactg ccggggattg tttcagtcaa agtactcgtc tggagcgatg
     4921 ggtctacgga tgagactgtt gccaaagcca aaacggctgg cgccgattat gtctttgcca
     4981 ataaaaaaaa cttaggcttg gcgcgtacct ttgatttagc caccaaaaaa gcggttgagt
     5041 tgggtgcaga catcgtagtg aatactgatg ccgataatca atacgatcag tcagaaattg
     5101 ctatgttggt cgcaccgctg attgcggaaa ctgccgatgt ggtcaacggt aaccggcaag
     5161 ttgagtctct gacgcacatg tctcactcaa aaaaatacgg caaccaactg ggaagctggg
     5221 tgatacggtg gctcaccggt ttgaaaatac aagatgcatc tagcgggttt cgtgcctaca
     5281 ccgcagaggc aattcgtaag cttacgatat tttccaagca tacctacaca cacgaaaccc
     5341 tcatccaaat cgcttttggc gaccttcaaa ttactgaggt accagttacc ttcaacgcgc
     5401 gccaatcgga ggccggacca tcccgcttgg tcagcaacgt atttacccat attacaaagt
     5461 ctggttcaac cattattcgt accatcttaa cgtacaaagc gcttagggtg tttattacca
     5521 tgggagcgct tgagcttgga ttggctgtat taattggttt gcgctacctt tggtttatgt
     5581 ttaatggttt gtccggtggc catgtccaat cgctgattct tgcctcgatg ctcttcaaca
     5641 tgggcttagt cagtatcctc attgggatta ttgccgattt aattgccgtc aacagaaaga
     5701 cacatgaatc aatttaaatt tgatatctgg acgctttggg gcctgacagc ccaaggattt
     5761 tacttcgcac gatttgtgtt gcaatgggtc cagtccgaaa gacagggtaa aattgtgatt
     5821 ccccatgtat tttggattct gagtttgatc ggcgcagtga tgatcatagt gtatagtttg
     5881 gttcggtcgg atttagtgct attaattact ggcctcctcc aactagtttt atttagtcgg
     5941 agtctcattc tttcaaaaaa attaaaaaag actacctaac atgcaaaaga aaccatttgt
     6001 tgaagggaac ttggaagata aatacaatgc caaaaatcct gtcagtaaat atttaatgca
     6061 gcgtttttta cgttcgtttg aacaactttt agtggaagta aaaaaacaag agcgaataac
     6121 cactatttgt gaagttggct gtggtgaggg cgaactgctg aaaatattgc atacacattt
     6181 tcccaaagct aaattgtatg cctgcgatct tgcttccaat gaaattgcca aagccaagtg
     6241 caacactcaa gagttaaaag taacattcac ccaacaaaat gctgagagtc tccatatcta
     6301 tgccgatcag caatttgatt tagtggtttg ttgtgaagta ctcgaacatt tagagcagcc
     6361 aaaaaaaggt ttggcggaac tgaaacgaat tggaagaacg gccgttgttt cagtgccagt
     6421 cgagccgctt tggagattcc tcaacctgtt gagattcaaa tacgtcaaac atctcggcaa
     6481 tacgcctggt cacctcaatc attggagtac agatagtttt caaaacctgc ttcgtaaacg
     6541 atcgctcaaa gtactacaat caaagtttcc tctgccttgg cagatgtatc tatttgaagc
     6601 aaaataaatt atgttcgtac cttggatttt tggcgacgaa tatctgtatc tcagcaaagc
     6661 gcggaatatt caacgtggaa ttgatgtatt agcggatgtg tcgcaaggtc acacctatcc
     6721 cccgctctat tcatatctct tatctctggt catgggcaca gatccgctga tttcgtatca
     6781 acacgctcag tggttgaatg tgggagtaag tcagctgctg attattttgg ccttactaat
     6841 gcttaatcgt gtttttagat ggactgattc aaagaagggc tgggtgtatt tgttgctgct
     6901 gtatcttgca gtggcgacga gcaccatgat tactggtttt tattttgtgg caatgagtga
     6961 aaatctgtat acaccattgg tggttttgat cttttcatta gtagtgtaca tctcaaaatc
     7021 gacgaagtta ctgacaaaac aaacttggct acttgttgga actgttggca tactgtgtgg
     7081 attggcggtg ttaacgagaa ccattggcta tgtgctattg ccatcagtga tggcgagctt
     7141 actttttgtt ggtatgccta acaagatcca acacaaatac aaaatcttaa ttgctctagc
     7201 ggtgggtttg attgccattg gtgccattca aactttcaat ctgtgggaag cgagccaagt
     7261 ggtgcgtact gagctgcaac aaacccacta tgaggaactt tctagtggtt atatgggggt
     7321 atttcaggca tttctgactg gtacggggaa ttggtttatg gctctcaaaa tattgggtaa
     7381 tcacctcacc tacttgctct ttgcgagttt ctttttccca ttactttttt tgatgaacga
     7441 tgtggtaaaa atgctaaaaa ctaagaagat tgatgccatg acagtctttg tcagtttgtt
     7501 tggattggga tcattagctt tttcttttct acattgttat tttggatttc aaactgagcc
     7561 aattaagtac tcaacgtatt ttcggtattt tgaccaaaca atacttattt ttattctgta
     7621 tggattgatt cggttgtggc agtggctaca aaataaggaa aagtttaata aattggctgt
     7681 cctgctgttt gcagttgtga gtttggtggg attggtattt ttgccaccga gagattttta
     7741 tatgaccttg aatgcttttg gctgggcatg gttggatttg tttattaata atcaatggat
     7801 gatacgcgtg gtgggctttg cactgattgt actgagcagt atgttggttt ggaaaaaaca
     7861 actctatcca ttgctcttag ttgtaataat cggttttcag gttgcctcaa ttcccacgat
     7921 cagtaagatg catgagtggc tatcacacga ttttaaggct ctggtggagc cagtcaggcg
     7981 ggtagcagtc gaccaaggaa ttaccaattg gtacataacc agcgattatc aggaaaaagg
     8041 catgctcggc gatctctatt tcataaaata tttgttgcta ttttatggca atgaatt
//
LOCUS       DAOQZP010000044         7968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_9533_length_7968_cov_4.409074, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000044 DAOQZP010000000
VERSION     DAOQZP010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9533_length_7968_cov_4.409074"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PKH60_03840"
     CDS             complement(<1..206)
                     /locus_tag="PKH60_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17206.1"
                     /translation="MQDAAHAFGRNHFGLANQNNHEPEQNGVNSHRLPQRVLVLRSDA
                     KREYFATDDHYLTESFSEENANMIV"
     gene            278..739
                     /locus_tag="PKH60_03845"
     CDS             278..739
                     /locus_tag="PKH60_03845"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ17207.1"
                     /translation="MDITCRIPTVDEIGQLADIIGEFEKDNLAKNYFSDQPSTNTELV
                     SYATDFINSESKRAFVAIANKRIVGFVLCILFTEDQHLEIIDLYIQPEFRKLGIGQKL
                     LQSVELIATKLDFLIKVEVYEKNNPAITFYQKLGYENDGVVLIKKISSHTV"
     gene            823..1515
                     /locus_tag="PKH60_03850"
     CDS             823..1515
                     /locus_tag="PKH60_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17208.1"
                     /translation="MEETVYSAEDSSQQRFQEVPKKSPRDLLQSFLTGSKGQPTAQTN
                     QARAPYPTPPGIEMSERFPGMVKPLPEELVYEWRSPSRPFKHRKKQFFTTLTTIVILL
                     CLILFFAGQVLPVAVVLAVGFLAYVLATIPPQEIVQQFTTYGIRIEKELYYWEELGRF
                     WFDKKLGSTLLIVEVARFPNRLTIVLKSADQAQEVEEILREVLLKQRPEKTWVEKAGD
                     WLQKKFPLDIDA"
     gene            1572..1643
                     /locus_tag="PKH60_03855"
     tRNA            1572..1643
                     /locus_tag="PKH60_03855"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1605..1607,aa:Arg,seq:gcg)
     gene            complement(1965..3098)
                     /gene="hflX"
                     /locus_tag="PKH60_03860"
     CDS             complement(1965..3098)
                     /gene="hflX"
                     /locus_tag="PKH60_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNQ17209.1"
                     /translation="MRQKRCLLVQVVNPEDPIDFAERNMRELEQLVATFGGEVVTKIV
                     QHRINPHPATYIGSGKLVELTEIVKLEKTEIVIFEGIVKASQLFRVEKRLWQVNPQIR
                     VWDKVDLILAIFDKHATTVEAKLQIELARINHLGPRSYGLGGGELSRQGGGIGTRGKG
                     ETNIEFERRVVRRRRQEIQQKLKKVTQQKQGRVQRRSELGLGPVALVGYTSAGKTTLF
                     NALTGKQKTVSKQLFTTLDTVVGKMKAPRGNLPVIISDTIGFIHNLPPDLIEAFQSTL
                     LESLTAKIILHVVDIRDPEFERNIEVVDEILAKMMVTQPVLLVGNKIDGVSKDKRVDF
                     RTRYQDREHILVSAVSGEGLQSLKDDIWKILSVESAYTSTSSV"
     gene            3147..3232
                     /locus_tag="PKH60_03865"
     tRNA            3147..3232
                     /locus_tag="PKH60_03865"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3181..3183,aa:Ser,seq:cga)
     gene            3348..4298
                     /locus_tag="PKH60_03870"
     CDS             3348..4298
                     /locus_tag="PKH60_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ17210.1"
                     /translation="MPNSHIIEVTNLVKRYKKAEVNSVDNVSFYVEDQAFFSLLGPNG
                     AGKTTTISILTTLLAITAGRATIAGFDVEKHPDKVRQQIGVIFQNPSLDLNLTAEENI
                     RFHVSLYNQFRYMPVFGLMSAEYKKKILTLAEVVGLEHDLDKPVKTLSGGMKRKLEII
                     RSLMHQPKVLFLDEPTTGLDPISRKNLWRYLQDIRKKEKITIFLTTHYLEEAEHSDYV
                     CIIDGGKIVAKGTPTEIKESLIKRYILVDSKDQIALKKELKALRLSFSGTGPFAISIK
                     SATGQEIVQKLTTSLTLLDIHNPTLEDAYVEIITKNNAQP"
     gene            4285..5064
                     /locus_tag="PKH60_03875"
     CDS             4285..5064
                     /locus_tag="PKH60_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ17211.1"
                     /translation="MHNLNAIFTIAFRDIIKLLRDRSRILASLIFPIIFIGVLGTSLQ
                     QNLGNNLTYNFLTFVFIGVLAQTLFQSTASGIISLVEDRQTDFAQEMFIAPISRYSIL
                     IGKILGETLVALVQIIGVILFAFVINIPLDLVSLLRMAPFFLVAAFFGGAFGVMVMAG
                     LSEQRAANQIFPFLLFPQFFLAGVFNPIQHLPLPIFIASRISPMTYAVDLLRSIYYFG
                     KPEYADVVLFNPLIDLTVVIGFGLLFVCIGTFLFVRNERNR"
     gene            5067..6293
                     /locus_tag="PKH60_03880"
     CDS             5067..6293
                     /locus_tag="PKH60_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HNQ17212.1"
                     /translation="MRYLIFICIVLASGWLKSSNSIPIDLDEIAWIHDAQVYQWRVTN
                     NWEHFNWNQKKKSMDWTSEDFRLFDQPHFAKYIYGFALSTANIVPWQNTNRENNFRLF
                     IAQKVSGHYLLTNDASSEIFGHATIESIKLCRWISLFFGLTTLLILFLFLTYKFSLLM
                     SSITLFLLTTNPTIFYNLHLATSDSISLFFIVISLILFYLLFFENHLTNKAGFILLFL
                     SAVSSAFAVSTKTNSWLLIYVLLLGQLLFCDRGRLVNWKETVTKTCLWFFLFVGTYIY
                     LQPELWTSPTAGFVRFFSQRLAQQNQFVHSFGTVGFFEYHKWLVELSAQSNHVVITII
                     KVTVLLIALIKFIKIFLTNFSKTNFLIKQLIIVTPIWLFFFFSARIGFDRYAIWPIII
                     LAIFSSWILTRKKLLI"
     gene            6301..7005
                     /locus_tag="PKH60_03885"
     CDS             6301..7005
                     /locus_tag="PKH60_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17213.1"
                     /translation="MSATQQEIALPNETSPLFCNDPTEFCRLFLVHLQNPDETPPQTS
                     TLAGRIVRTKTAIEASLLADEPGRDIVFCQPSDSLNQSAKSTDFADILPSLGFTRNEI
                     NDYLANGYHFYLVVFPKDEMFLADWDSLRLLFSQRYPELATTIAALWDETKKVPFDQI
                     DSSISLPRFLSIPKEQRTAKQLRGLLFKLWRLNKDFTGTGLTSGGLPEMLMLNQIIST
                     IKNVSWARLDVRPYTD"
     gene            7046..7855
                     /locus_tag="PKH60_03890"
     CDS             7046..7855
                     /locus_tag="PKH60_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNQ17214.1"
                     /translation="MFEVSSLHPILQAFLAGLFTWGVTAVGASGVFFFSKINQKLFNG
                     LLGFAAGVMIAASFWSLLAPSIALSKELGNQSWVAPAIGFILGGCFLWILDKIIPHLH
                     IGSPVNAKEGLSTRWKKTVLLFSSITLHNIPEGLAIGVAFGAVAAEIPGASLASAIAL
                     AFGIGIQNFPEGFAVSMPFRREGLTKMRSFLLGQASAMVEPIAAVIGAALVIVMRPAL
                     PYALAFAAGAMIFVTVEEIIPESQSEGSSDFATAGAMLGFVVMMILDVALG"
     gene            7881..7968
                     /locus_tag="PKH60_03895"
     tRNA            7881..7968
                     /locus_tag="PKH60_03895"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7915..7917,aa:Ser,seq:gct)
BASE COUNT         2267 a         1675 c         1491 g         2535 t
ORIGIN      
        1 acaatcatat tggcattttc ctcagaaaaa ctctctgtga gataatggtc atcggttgca
       61 aaatactctc ttttggcatc agaacgcaaa acaagcactc gttgaggaag cctgtggcta
      121 tttacaccgt tttgttctgg ctcatgattg ttttgattag ctagcccaaa gtgattccta
      181 ccaaaagcat gtgcagcatc ttgcatagtt actcaatttc taaatatttc tcatcaacta
      241 ctacgatagt ataaatattt gttatacact aattcctatg gatattacat gtagaattcc
      301 aacagtagat gaaattgggc aacttgcaga tatcatcgga gaatttgaaa aagataatct
      361 agctaaaaac tatttttctg atcaaccatc aactaacact gaactagttt cttacgccac
      421 agattttatt aattctgaaa gcaagcgagc attcgtggct attgcaaata aaaggatagt
      481 tgggtttgtc ttatgtattc ttttcactga agatcagcat ctagaaataa tcgatttata
      541 cattcaacct gagtttcgca aactcggtat tggccagaaa ctgttacaat cagttgagtt
      601 aattgctaca aaactagatt ttcttataaa agtcgaagtt tacgaaaaaa ataatcctgc
      661 cattaccttt tatcaaaaat tgggatacga gaacgatgga gtggtgttaa ttaaaaagat
      721 ttcttcccac acagtttagt taattggtct atacataaaa tgattctgat agttattaag
      781 tcataattgc atttgtacct agatctcttg tacacttaga gtatggaaga aacagtgtat
      841 tctgctgaag atagtagtca acagcgtttt caagaggtac ctaaaaagtc tcctcgagac
      901 ttgctgcaat catttttaac tgggtctaag ggtcaaccga cagctcaaac aaatcaagca
      961 cgtgccccgt atcctacacc gccagggatt gaaatgtccg agcggtttcc cggcatggtc
     1021 aaaccattac cagaagaatt ggtttacgag tggcgttctc caagcagacc ttttaaacat
     1081 cgcaaaaaac aattttttac tacactgact acaattgtaa ttttactttg ccttattttg
     1141 ttttttgctg ggcaagtatt gccagtcgca gtggtgctag cagttggttt tttggcctac
     1201 gtactggcga ccataccgcc acaggaaatc gttcaacaat ttactaccta tggtatacga
     1261 attgaaaaag agctgtatta ctgggaagaa cttggtcgtt tttggtttga taaaaaatta
     1321 ggctcaacac tactgatcgt agaagttgca cgctttccca atcggctcac tatagtgctc
     1381 aaatcagcag atcaagcaca agaagttgaa gaaattctcc gcgaagtttt gttaaaacaa
     1441 cgaccagaaa aaacttgggt ggaaaaagct ggtgattggt tacaaaagaa atttccatta
     1501 gatattgatg cctagttttt ttctttactt agtctctggt ggtttctggt acaatacctc
     1561 ctgctaacag tgctcccata gtttaaggga tagaacggtg gtttgcggaa ccactaatcc
     1621 aggttcgagt cctggtggga gcacacttca acccatcttt tcaggtcatc ccgcctaatc
     1681 ccgaccatat gtggtaaagc tttctccaaa aagtaaagcg attttataaa agtaaagtag
     1741 tcctatggta agcattgaac cgtagtaaga atctggttga tgagggacct catgttttgg
     1801 aaacatagtt gacactaaag caaagctaca gacagggtca tgctttagtc taaactatca
     1861 aatgaaacca agtggtgtat gtaacggcca ggcacactac acaaaccagc tttctaaggg
     1921 tttccttcga caagaatgta actctgacaa ttcatcaaag tctgtcacac tgagcttgtc
     1981 gaagtgtagg cagactcaac agataaaatt ttccagatat catccttcaa actttgcaag
     2041 ccttctccgg atactgctga tactagaata tgctcacgat cctgatatct tgtcctaaaa
     2101 tcgactcgtt tgtcttttga tactccatcg attttatttc ccaccagtag tactggttga
     2161 gtgaccatca ttttcgccaa aatttcatct acaacttcaa tgttccgctc gaattctgga
     2221 tcacgaatat caaccacatg caaaattatt tttgccgtta atgattctaa cagcgtggat
     2281 tggaatgcct caatcaaatc tgggggaaga ttatgaataa atccaatcgt gtcggagata
     2341 atgactggca ggttacctct cggagctttc attttcccaa ccactgtatc taaggtggta
     2401 aatagttgtt tactcactgt tttttgtttg ccagtcaatg cattaaacag agtagtcttg
     2461 ccggcgctgg tgtaccccac cagcgccacc ggacccaaac ctagttcgga tcttcgttgc
     2521 actctaccct gtttttgttg agttactttc ttgagtttct gttgaatttc ttggcggcgg
     2581 cgcctgacaa ctctccgctc aaactcaata ttagtctcac ctttgccacg ggtaccaatg
     2641 ccgccgccct gcctagaaag ctctccaccg cctaatccat aacttcttgg acccagatga
     2701 ttaattctgg ctaattcaat ctgtaatttt gcttcaacgg tggtcgcgtg tttgtcaaaa
     2761 atcgctaaaa tcaaatccac tttatcccaa actctgatct gcggattaac ttgccaaagc
     2821 cttttttcaa ctctaaataa ttgactagct ttaacaatac cttcaaaaat gacaatctcg
     2881 gttttttcta acttcacaat ttctgttaat tcaactaatt ttcccgatcc aatataggtg
     2941 gcaggatgag ggttgattcg atgttgaact atttttgtga ccacctcacc accaaaagtt
     3001 gccaccaact gctccaactc acgcatattt ctttcagcga aatcaatagg atcctcgggg
     3061 ttaaccacct gaactaatag acagcgtttt tgcctcatga gcgcattgta ccaaaattaa
     3121 agaatcctat ataatgagca catattggaa gcgtcgcata gtggttaatt gcactggttt
     3181 cgaaaaccag agcctcacgg cttcgtgggt tcgaatccca ccgcttccgc cattcttcga
     3241 caatctcaga atgacatcag tgtgacgaat gagaatggtg ggatgaaaag tcaatctaca
     3301 agattttaca ggaccaatga tatactgctt gccagtacat tacaccgatg ccaaactctc
     3361 acatcatcga agtcaccaat ctcgtcaaac gctacaaaaa agccgaagtg aattctgttg
     3421 acaatgtttc attttacgta gaagaccaag ctttcttctc attactcgga ccaaatggtg
     3481 caggtaaaac tactactatt tcaatcttga ctaccctcct tgctatcaca gctggtcggg
     3541 cgaccattgc gggttttgat gttgagaagc atccagacaa agttcgtcaa caaattgggg
     3601 tgatttttca aaatcccagt ttggatctca atctaactgc cgaagaaaat attcgtttcc
     3661 atgtcagttt gtataaccaa tttcgataca tgccagtatt tggtctcatg tctgcagaat
     3721 acaaaaaaaa gattctaacc ttagctgaag tagttggctt ggaacatgat ttggataaac
     3781 ctgttaaaac actctcggga ggaatgaaac gaaaattaga aattatacgc agtctcatgc
     3841 atcaacccaa agtcttattt cttgatgagc caacaacggg actagaccca attagtcgga
     3901 aaaatctttg gcgctattta caagatattc ggaaaaaaga aaaaatcacc atctttttaa
     3961 ccactcacta cttagaagaa gccgaacact cagattatgt ctgtattatt gatgggggca
     4021 aaattgttgc caaaggcacc cccacagaaa ttaaagaatc attaatcaag cgatacattt
     4081 tagtcgattc aaaagaccaa attgctttga agaaagaatt gaaagctctg cgactttctt
     4141 tttcgggaac tggaccattc gcaatcagta ttaaaagtgc aacaggtcaa gaaatagtac
     4201 agaaacttac cacttcgttg accctattgg atattcataa tcctaccttg gaagacgcgt
     4261 acgtagaaat aattacgaaa aataatgcac aaccttaacg caatttttac cattgctttc
     4321 agagatatca tcaagctcct gcgtgataga tccagaatct tggcaagcct aatttttcca
     4381 attattttta ttggagtttt aggaacgagc ctacaacaaa atctagggaa caatctcacg
     4441 tataattttc tgacttttgt cttcattggc gtactagctc aaacactgtt tcagtcgact
     4501 gcttctggca taatttcact ggtagaagat cgacaaactg attttgctca agaaatgttc
     4561 atcgctccaa tttctcgcta ctcaatttta attggaaaaa tcttaggaga aaccctcgtg
     4621 gctttagtgc aaatcattgg cgtgatttta tttgcattcg taataaacat acctcttgat
     4681 ttggtgagcc tgctacgaat ggctcctttc ttcctcgtgg cagcattttt tggtggagcg
     4741 tttggcgtga tggtgatggc aggattgagc gaacagcggg cggcaaatca gatctttcca
     4801 tttttactct ttccccagtt tttcttagca ggagtcttta atcccattca gcatttgccg
     4861 ctaccgatct tcattgcgtc tcgaatctca ccaatgacat atgctgttga tttgcttcgg
     4921 agtatttact actttggtaa accagaatat gccgatgtag tgctgtttaa tccactgatt
     4981 gatctcactg ttgtaattgg ttttggtcta ctctttgttt gtatcggaac gtttttattc
     5041 gttcgcaatg aacgcaatcg ataacaatgc gatatcttat ttttatttgt attgttttag
     5101 ctagcggctg gttaaagagt agtaattcca ttccgattga tcttgatgaa atcgcttgga
     5161 ttcacgacgc gcaagtgtat caatggagag taaccaacaa ttgggaacat tttaattgga
     5221 accaaaaaaa gaaatcgatg gattggactt ctgaggattt tcgtttgttt gatcaacctc
     5281 atttcgcgaa atatatctat ggatttgcat tatctacagc aaatattgtt ccctggcaaa
     5341 atacaaaccg cgaaaacaac tttcgtctct ttattgcaca aaaagtttct ggccactatt
     5401 tactcacaaa tgatgctagc tcagaaattt ttggccatgc tacaattgaa tctattaaac
     5461 tttgtcgatg gattagttta ttttttggtt taaccacttt actgattctt tttctcttct
     5521 taacttacaa gttttccttg ttaatgagtt cgatcacttt atttctgctg accactaatc
     5581 ccactatttt ctacaatctt catctggcca ccagtgactc aatatcacta tttttcatag
     5641 tcattagtct tattttattt tacttgctgt tttttgaaaa tcatttaacc aataaggcag
     5701 gatttatact tctctttcta tctgctgtat cctccgcttt tgcagtcagt accaaaacta
     5761 atagttggtt gctgatttat gttcttttac taggacaact cctattctgc gatcgtggtc
     5821 gattagtaaa ttggaaagaa actgttacaa aaacctgtct ctggtttttt cttttcgttg
     5881 gtacctatat ttatttgcag ccagaattat ggacctcacc gactgctgga tttgttagat
     5941 ttttttctca acgtttagct cagcagaatc agtttgtgca ttcattcggc acagtaggtt
     6001 tttttgaata ccacaagtgg ttggttgagc tgagtgctca gtcaaatcat gtcgtaataa
     6061 ctatcatcaa agtaactgtt ctactaattg cattgattaa atttatcaaa atattcctca
     6121 ctaatttttc aaaaacaaat tttctaatca agcaattaat tattgtcacc cctatctggc
     6181 tcttcttttt tttctctgcc agaatcggat tcgatcggta tgcaatttgg ccaattatca
     6241 ttctagccat cttttctagt tggatactta ctcgtaaaaa actgctgata taattatttc
     6301 gtgtcagcca cgcaacaaga aatcgcttta ccaaatgaga caagtccatt gttttgtaac
     6361 gatcccactg agttttgtcg attgtttttg gtgcatctac agaatcccga cgaaacccct
     6421 ccccagacct ctactcttgc tggcagaatt gttcgcacta aaacagctat tgaagcatct
     6481 ttgctcgcag atgaaccagg aagagatatc gttttttgcc aaccaagtga ttcacttaat
     6541 caatctgcaa aaagtaccga ctttgccgac attcttccct cacttggatt taccagaaac
     6601 gagattaatg actatttagc caatggctat cacttttact tagttgtttt tcccaaggac
     6661 gaaatgtttc ttgcagattg ggattcctta cgattgctct ttagtcaaag atatcctgag
     6721 ttagcaacga caattgccgc actttgggat gaaaccaaaa aagtgccatt cgatcaaatt
     6781 gattcttcaa tttccttacc caggttttta tcaattccca aagagcaacg gacagccaaa
     6841 caattacgtg gtctgctctt taaactctgg cgattgaata aagattttac tggcaccggt
     6901 cttacttcgg gcggtcttcc agaaatgttg atgctcaacc aaataatctc aacaatcaaa
     6961 aacgttagct gggcgcgact ggatgttcga ccgtatactg attgaataaa tttcatatat
     7021 cacctgtgat acgatagaca ctattgtgtt tgaagtttca tcgttacacc cgattttaca
     7081 agctttcctc gcaggtcttt ttacttgggg agtcactgcc gttggagcat caggtgtttt
     7141 cttcttttca aaaatcaatc aaaaattatt taatggccta cttggctttg ccgctggtgt
     7201 catgattgcg gctagttttt ggtcattact tgcaccatcg attgccctaa gtaaagagtt
     7261 gggcaaccaa tcatgggttg cgccggcaat tgggtttatt cttggcggtt gttttctctg
     7321 gatattagat aaaattattc ctcacctgca tattggctca ccagtaaatg caaaggaagg
     7381 actttcgact cggtggaaaa aaactgtact tttattttca tctattactc ttcataacat
     7441 acctgaagga ttagctattg gagtggcatt tggtgcggtg gcggcagaga ttccgggcgc
     7501 aagcttggcg agcgccatcg ctttagcctt tggcattggt attcaaaact ttccagaagg
     7561 ctttgctgtt tcgatgccat ttcgcagaga aggtttaaca aaaatgagaa gttttttact
     7621 tggtcaagca tctgcgatgg tagaaccaat tgcggcagtt attggcgccg cactggtgat
     7681 tgtaatgcga ccagcgctcc cctatgccct agcatttgct gctggcgcca tgatttttgt
     7741 tactgtagaa gaaatcattc ccgaatctca atcagaaggc agcagtgatt ttgccactgc
     7801 cggtgccatg ttgggctttg tagtgatgat gatcttggat gtagcgcttg gctaggttac
     7861 aatacagccc tagttagatt ggagacgtcg catagtggcc aattgcaaca ggttgctaac
     7921 ctgtaccggt tttccggtcc gtgggttcaa atcccaccgt ctccgcca
//
LOCUS       DAOQZP010000045         6916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_11327_length_6916_cov_6.761842, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000045 DAOQZP010000000
VERSION     DAOQZP010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6916
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11327_length_6916_cov_6.761842"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4631)
                     /locus_tag="PKH60_03900"
     CDS             complement(<1..4631)
                     /locus_tag="PKH60_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HNQ17215.1"
                     /translation="MLAQNMYIHHLAALFVIYTQVHHHNQNSEQEIQKAIRSLLEEYN
                     QAQSDNRLPAWENEPASTKLPTLLQQFAKSYQLSQIGVALHKYLLALQTAGRLPATIK
                     NYRSDIGQYLSFISSNSPDQIVQKTEVTHFIEYELTRGSSKSTIQRKLASLGQFFQWL
                     QENGWLTTNQWKSFSAQEFTELLKSAKSVRELTTPVSQVDERPAIWSLPLKNIDVQQY
                     LPKHFANSPIKKIKKAPFFSRIKQAMGAQPLLGYFNLAMGILFLLGTAFLGYQQFFSG
                     ADSSQAFPANLTRPNRSLSFQGRLTSSSRTPITSATPMRFRLYDSGPATGSGNMLWDS
                     DSCNIDPDQDGIFSTTLGDACGAEITSDVFTENSNVWLEVQVNSEILNPRQSIKTVAY
                     ALNSETLQGYPASASAVESTVVVMRSDGKVVFGNANPILEATGDSFAITAKTLTLETS
                     SGSNGNVIINPDGTGQTLINSNTLIEGYLSAPGATLSATYAGGRALTLKAGPSATGDI
                     MQWQTSVGTPLGVIDKSGNIGIGTTVARASLEIAGTASSSGTLTFKGTTDPKIDILNG
                     ENFGLRTSVGGDVGLTERLTVLNSGNVGIGTTAPGAKLAIANTGTANGIILGSDQGTP
                     VNIYHSNSPVDGVLYLSGSRIQLNGNYTTINSTYTSLGSYISNSPIVLGGQNLGFNND
                     NNNDRMRIKGGIGSENALRFLLSDASTEVFRINSTGTASSSGVLTFRGTTDPKINVLN
                     GENFGIQTSVGGDVGLAERFTILNNGYVGINSTSPSQQLEVGGNVEIANYLYFANGAT
                     NYLRFDGSDFLLSNDFLPSANNTYALGSNAARWSAIYGTAIYQGGNQVCDTSGNCSSV
                     GNLWQYANGGISPATPYTSVADFYIGGNATASAKFGLLNVNSGTPTASISAGAAGATF
                     LTASGYLSTTARQNLTLGNSSTYNTTGNILLNPNGTGNVGIGTTNPAYRFHVSGGTLA
                     VDSGSGTDTLRIRDGSITKTVGDYFTWDTGIKLNTGSYNQIIFGTTTPAANGSGLVSS
                     GDMSFWTSGYTNRPMYISSAGNVGIGTTTPTALLDIAGTASSSGTLAFRGTTDPKIDI
                     LNGENFSIRTSVGGDVGLTERLTILNGGNVGIGSTAPAQRLDVVGNLQFSGALMPNAQ
                     AGTSTYLLTSAGANNPPTWQNPATFISQYDLWSLSSGAIFPKNSTTDLLVGGNSTASA
                     KFGFINVNSGTPTASISGTIGATFLTANGNLSTTNRQSLTLGNSAAYNTTGNILLNPN
                     GTGNVGIGTTNPTSPLQVGSAGSGRISVPGLGGNSEQFGYLADADGIGATALGNSSSA
                     GGQSSMAIGYSTSASGVRGLAIGYDATNTGNDSMVIGNSASVGHNSIVIGQSASTTQS
                     YAIVLGRSATVTGTAGIAIGESAAAGANQFVSGSSSYPINDVYFGKGVTNATPTEYTL
                     NGTGGSGTNIAGGNLTIAGGKGTGNALGGSLLFKTSDAGVSGSGLQSLTTKMTILAGG
                     NVGIGSTSPGYPLDVNGRVRMTDFQLNKAPLAAGYALISDASGVGTWTD"
     gene            5149..5901
                     /locus_tag="PKH60_03905"
     CDS             5149..5901
                     /locus_tag="PKH60_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17216.1"
                     /translation="MKNNTRKLLLFLYPNTKERLALPVESLELICSELTASGRQSLLR
                     LLEKRGWILSLLQPNGSLLISLTSLGKAALEREFPVPLTKNSKDSEIWTAILFCVSSK
                     SDPQFRNLRRMILSNGGLALTRGMYVFSGSLPIKLQTTISSLYGQQVSIFTISEWIFG
                     KDPSLLFDKSGLARVVEIYSSISKELDELLSILYSEKGLNNKRKRQISTVLERVYRNV
                     LEDSAMVHRFYPDAVPMITILQKIQRLILAMF"
     gene            complement(6138..>6916)
                     /locus_tag="PKH60_03910"
     CDS             complement(6138..>6916)
                     /locus_tag="PKH60_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17217.1"
                     /translation="LRPSEQSPLQPFITLFNRPKSDLVIKRGKDKVTFITEERRPFWE
                     WLSPAMLLLPTLIMFIILICQFGRVPDITMIKIIIWWFGWRNKQKPDGEVHDRSSLKP
                     LVLIPIEVVNPVSKESTIVSVTNRSGEFLLPKLEPGDLILNPKWSGLQFPSIAKRPQS
                     VPWHRFYLGEIVSYNNDLPWPFVHIPVEQLKKQSSWYKLIIESTQWRGPLFTIQFAVI
                     VIIVLLSPSLLTGTPLLICSLLGFFRFFSNKNSHLANNTA"
BASE COUNT         1956 a         1429 c         1556 g         1975 t
ORIGIN      
        1 atgtccgtcc aggtgcccac gccactagcg tcagaaatta aggcgtaacc agccgctaac
       61 ggggctttgt tcagttgaaa gtcagtcatc ctgactcgac cattgacgtc aagcgggtag
      121 ccagggctgg ttgagccgat accgacgttg ccacctgcca atattgtcat ttttgtcgta
      181 agcgactgta gtcctgagcc actaactcca gcatcagaag tcttgaatag caaacttcct
      241 cccagtgcat taccagtccc tttaccacca gcaatagtta aattgcctcc agcaatgttt
      301 gttccagatc cacctgttcc atttagtgtg tactcagttg gcgtcgcgtt cgttacacct
      361 tttccaaaat acacatcgtt tattggatag cttgatgatc ctgaaacaaa ctgatttgcg
      421 ccagctgctg cagattcacc aattgcaatg ccagcagtac cagtaaccgt cgccgacctt
      481 ccaagcacaa ttgcataact ttgggtagtt gaagcacttt ggccaattac tatggagtta
      541 tgtccaacac tagcagaatt accgataacc atggagtcat ttccggtatt ggttgcgtcg
      601 tatccaattg ctagtcctct tactccagat gcagaagtac tatacccaat tgccattgaa
      661 ctttgaccac cagcgctaga agaattacct agagcagtag ctcctattcc atcagcatcg
      721 gccaaataac caaattgttc tgagttgcca cccaaccctg ggacgctaat tctgccactt
      781 ccagctgaac ccacttgtag tggacttgtt gggttggttg tcccaattcc cacatttccc
      841 gtcccattgg gattaagcag gatgttgcca gtggtgttgt aggcggcgga gttgccgagg
      901 gttaagcttt gtctattggt agttgaaagg ttgccattgg cggtcaggaa ggttgcgcca
      961 atagttcctg agattgaagc agtgggtgtg ccgctgttca cattgatgaa accaaacttg
     1021 gctgatgccg ttgagttgcc gccgacaagc aggtcggtag ttgagttttt ggggaaaatg
     1081 gcgccactac ttaagctcca gagatcatat tgagaaatga atgttgctgg attttgccat
     1141 gtgggagggt tgtttgctcc ggctgaagtg agcaagtagg tgctagtacc agcttgtgca
     1201 ttgggcatca gagctccact aaattgcagg ttgccaacaa catcgagtct ttgagcggga
     1261 gcagtggagc cgataccgac gttgccacca ttcagaattg tgagtctctc tgttaatcca
     1321 acatctccac caacactggt tcggatgcta aaattttcac catttaaaat atcgattttg
     1381 ggatcagtcg tacctcgaaa ggcgagggtg ccagaagaag aagcagtgcc agcaatatca
     1441 agaagcgctg ttggggttgt ggtcccaatg ccgacattac ctgcggagga aatatacatt
     1501 ggtcgatttg tgtagccgct agtccagaaa gacatgtctc cactacttac taaaccagac
     1561 ccatttgcag cgggagttgt tgtaccaaaa atgatttgat tataactacc agtatttaac
     1621 ttaattccgg tatcccaagt gaaataatca ccaacggttt tggtaataga tccatctcta
     1681 atgcgaagtg tatcggtgcc tgacccacta tctactgcaa gagtgccacc agaaacatgg
     1741 aagcggtagg caggattcgt cgtcccaatg ccgacgttac cagtaccatt aggattaagc
     1801 aggatgttac cagtggtgtt gtaggtactt gaatttccga gggttaagtt ctgcctggcg
     1861 gtggttgaga ggtaaccact ggctgtcagg aaggttgcgc cagcagcccc agcagagata
     1921 ctagcagtag gcgtgccact gttgacattg aggaggccaa actttgccga agcagtggcg
     1981 ttgcctccaa tgtaaaaatc cgctactgag gtatagggag tagcagggga gataccacca
     2041 ttggcatatt gccatagatt gccaacactc gaacagttac cactggtgtc acaaacttgg
     2101 ttaccacctt ggtaaatggc ggtgccgtag atggcggacc aacgcgcggc gttgcttccc
     2161 agagcatacg tattattggc actcgggaga aaatcattac tcaataagaa atctgaaccg
     2221 tcaaacctca agtaatttgt tgctccattg gcaaaataaa gataattggc aatttctacg
     2281 ttaccaccaa cctcaagctg ttgacttgga gaagtagagt taatgcctac atagccgtta
     2341 ttgagaattg tgaaacgctc tgccagtccg acatcgccac caactgaagt ctgaatacca
     2401 aagttctcgc catttaagac gtttattttg ggatcagtgg tacccctaaa ggtcaacact
     2461 cctgatgacg atgcggtgcc agtggagtta attcggaata cctcagtaga ggcgtcactt
     2521 aataggaatc taagtgcatt ttctgaacca attcctccct taattctcat tctgtcatta
     2581 ttgttatcgt tgttaaaacc aagattttgt cctcctaaaa ctattgggct atttgaaatg
     2641 tatgagccta aggacgtgta tgttgaattg atggtggtat agttaccgtt tagctgtatc
     2701 cgagacccac ttaaatataa aactccatct actgggctat ttgaatggta gatatttact
     2761 ggggtgcctt gatcactacc caaaataatt ccattagcgg ttcctgtatt ggctatggct
     2821 aactttgctc caggcgctgt cgtcccgatc cccacattac cgctgtttag cacggtcaac
     2881 ctctccgtta ggcccacatc accacccaca cttgttctca aaccaaagtt ttcgccgttt
     2941 aagatatcga tcttgggatc agtggtgcct ttgaaggtga gtgtgccaga agaagaagcg
     3001 gtgccagcaa tctcgagtga agcccgagcg acggtggtgc caataccaat attgccactt
     3061 ttatcgatca cgcccagtgg tgttcccact gaagtttgcc actgcataat atcgccagtc
     3121 gcacttggac cagccttgag agtgagagct ctaccgccgg cgtaggtagc ggatagggtg
     3181 gcgccaggag cggaaagata gccctcgatc agggtgtttg agtttataag tgtttgaccc
     3241 gttccgtcag ggttaataat cacattgcca ttacttccag atgatgtctc aagtgtgaga
     3301 gttttggcgg taattgcaaa cgaatcgcct gtggcttcta aaatcgggtt tgcattgcca
     3361 aagacaacct tgccgtcgct tctcatcaca acgaccgtac tttcaaccgc actcgctgaa
     3421 gcggggtatc cctgcagtgt ttcagaatta agtgcatagg ccaccgtctt tattgactgg
     3481 cggggattta gaatttcgct attaacctgc acttccaacc acacattgct attttctgta
     3541 aacacatcag aagtgatctc tgcaccacac gcatctccaa gtgttgtgga gaaaataccg
     3601 tcttgatctg ggtcaatgtt acaactgtct gagtcccaaa gcatgttgcc actgccagtg
     3661 gcaggccctg aatcgtagag tcgaaagcgc attggagtgg cagaggtgat tggggttctg
     3721 ctagagcttg ttaagcgacc ttgaaacgac agtgatcgat tgggtctggt gaggttggct
     3781 ggaaatgctt gtgaagaatc tgccccagag aaaaactgtt ggtatccaag aaatgcggtc
     3841 ccgagtaaaa acaatatacc cattgccaga ttaaaatagc caagtaatgg ttgagcgccc
     3901 atcgcttgtt taatccttga gaaaaatgga gcttttttaa tcttttttat cggtgagttg
     3961 gcaaagtgct tgggaagata ctgttggacg tcaatatttt ttaagggcaa tgaccagatt
     4021 gctggtcgtt catcaacttg actaactgga gtagtaagct cacgtactga tttggcactc
     4081 ttgagtaatt cagtaaattc ttgggcagaa aatgacttcc actgattcgt agtcaaccat
     4141 ccattctctt gcaaccactg aaaaaactgc cctaaactcg ctaattttcg ttgaatagtc
     4201 gacttggagg aaccacgtgt aagctcatac tcgatgaagt gagtaacttc ggttttttgc
     4261 acaatctgat ccggagaatt agaagagata aaactcaagt attgtccgat gtcagagcga
     4321 taatttttta ttgtagctgg taagcggcca gcagtttgca gtgccaataa gtacttgtga
     4381 agagcaactc caatctggga gagttggtaa ctctttgcaa actgctgtaa aagcgtcggc
     4441 agttttgttg aagcaggctc attttcccat gcaggcaagc gattgtctga ttgagcttgg
     4501 ttatattcct caagcaagga ccgtatagcc ttttggattt cttgttcgct gttttggttg
     4561 tggtgatgta cctgtgtata aatcacaaac aaagctgcga gatggtggat gtacatgttt
     4621 tgggctagca tgaaggaatt ttatgatcag tcagctttct cggtattaga caattagatg
     4681 atctggataa tcttggcaga aattcaactt gatgtcaaag tctggaaaac aagatgtttt
     4741 ctaggctagt aactctagat gtgcataact gaggttttcg gtgataaaaa gcagaataat
     4801 aaatgacttc acgtcatttc atgattatca gattgtttat atgttcggta tttatatgca
     4861 acaaagtctg gaaatttggt aataatccca acttgacaac ttcatttgtg aaattgtaca
     4921 ttgtattaat acaagcaacc taagtgaaca tatttctact atgttttatg aaaatcactg
     4981 gtagccagag taaaaagata aatcattggg ttgaaattac tttttatacc gaaaagttgt
     5041 gattcgggtt acaaaattgt gaataactcg aaaatgatgt ctaaatagcg tatatatatt
     5101 aacgaaataa atacagatat agaatgtcat acaactgcat acataaatat gaaaaataat
     5161 acccgaaaac tattgctttt cctctatccg aataccaaag agaggctggc gctaccagtt
     5221 gagtcgcttg agctgatttg ctctgagcta accgcatcag gaagacaatc gttgctccga
     5281 ctgttagaga agcgaggttg gatcttatcg ttacttcaac caaatggatc actattaatt
     5341 tcattgacta gtttaggtaa ggccgcacta gaacgcgaat ttccggttcc gcttacaaag
     5401 aactccaaag actctgagat ttggactgcg atcctttttt gcgtctcttc aaagagtgac
     5461 ccccagttta gaaatctaag gagaatgatt ctttcaaatg gaggattggc acttacaaga
     5521 gggatgtatg tattctctgg ctctttacca attaagctcc agactactat ctcaagtctc
     5581 tatggccaac aggttagtat atttactata agcgagtgga ttttcggtaa agatccgtca
     5641 ttgttgtttg ataaatctgg ccttgctcgg gtggtagaga tatactcaag tattagcaaa
     5701 gagttagatg aattgctaag cattttatat agtgaaaaag gtttgaacaa taaaagaaaa
     5761 aggcaaattt caacagtatt agaaagagtg tatcgaaatg ttcttgagga ttctgccatg
     5821 gtacatcggt tttatccaga cgctgttccc atgataacta ttttacaaaa aattcaacgt
     5881 ttgatactcg ctatgtttta aacctaatta ctataggtat ttatttgatt caggattgcg
     5941 gtaatcattt ggtggtgaat tcaaataact tatgaattga ggagcatcct ttcggtacag
     6001 caaaattcta tgctctggca gatgtcaaaa gatatataaa agtctacaaa taacaaaaaa
     6061 aactataatg caacaaatag ctacaagata agcgataaaa acgcaggaaa aatgcggaaa
     6121 aacctaaagt aaggaagcta ggcagtatta ttggcaagat gactattttt atttgagaaa
     6181 aagcgaaaaa aacccaaaag agagcaaatt aataatggag tgccagttaa cagtgatgga
     6241 ctaagaagaa caataatgac tatcactgca aactggattg tgaacagtgg accacgccac
     6301 tgtgtactct ctatgatcaa cttataccaa ctggattgtt tcttcagttg ttcaacagga
     6361 atgtgaacga atggccatgg taagtcgttg ttataactta ctatctcacc aagataaaaa
     6421 cgatgccagg gaacagattg cggacgtttc gctatacttg ggaattgtaa acctgaccat
     6481 tttggattta aaataagatc cccaggttct agtttgggta gtaggaattc tccggagcga
     6541 ttagtgaccg agacaatcgt tgactcctta ctcactgggt taactacttc aattgggatc
     6601 aacactagtg gttttaggct ggatctatca tgcacctctc catctggttt ctgtttattt
     6661 ctccaaccaa accaccaaat aattattttt atcattgtta tatctggcac ccgaccgaat
     6721 tggcatatga gaattataaa cattattaaa gtgggcagta gtaacattgc tggtgataac
     6781 cattcccaga atggccttcg ttcttcagtg atgaatgtta ctttgtcttt acctcgctta
     6841 atgactaaat ccgattttgg tctattaaac agcgtgataa agggttgtag tggtgattgc
     6901 tcagatggtc gtaaag
//
LOCUS       DAOQZP010000046         6712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_11772_length_6712_cov_4.205348, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000046 DAOQZP010000000
VERSION     DAOQZP010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11772_length_6712_cov_4.205348"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="PKH60_03915"
     CDS             complement(<1..1376)
                     /locus_tag="PKH60_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17218.1"
                     /translation="MIDTEPKGAYTLADFSKALADEMRPDGGKSPEESKRRLVDILLH
                     LNKEVKLPTRFKQILLERFDIQESDHSATAVETVLPEVAEVGLPSVAEAESPLVEGEV
                     LILPDEVLKQLDLIDVAINAIEDEVAMEKMTSDETLRKITQNIVRQTVMPLFRKLVQV
                     QKLLPKEIFDLVEIEKAKRMVIIEEKISGVTPVESSADVASIKKDEIDPTDFWSSFVR
                     TKYPDVLTTPDSRGESRAEVLMVCDRIFSEYVSYEAHYLWKDPMDRHTAETEANFSAA
                     PIYTKNQTLNYSKLFDTIPIPDIQKEFKEKLELCRKTYLAGSEWKAVIAEQPVPGSYE
                     KFGSFFSSQLPTGSDWRVVQELPKLLDKDGKQSETSFGEQVFHSQRLYSTYNRRYVYA
                     NEGEKAEVARILQDEIVSGRVIIPKPTDVVAILTAEEKIERQRRLGKLLPKKLLGGSA
                     TPAINMGES"
     gene            1494..1793
                     /locus_tag="PKH60_03920"
     CDS             1494..1793
                     /locus_tag="PKH60_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17219.1"
                     /translation="MSERVTIPPNFVDHIMGGDICPELLLLMLSIQRDFDEQQVTLER
                     RLLNLSACECDRCWDLYWARWNELRSRDDNLQDLQEDESVLEDIHRYAPKLEEVD"
     gene            1842..6440
                     /locus_tag="PKH60_03925"
     CDS             1842..6440
                     /locus_tag="PKH60_03925"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNQ17220.1"
                     /translation="MADTQTGVGVVDLPPNVINLADIKRLQLNSLEDVINATKLLSRV
                     VAEKKEELLFEKQSVTLKLTSGVFSPQTTNKWWEQEVVDNPNNFLLTTDFTNFIAQID
                     LWLVQHAEDIQKKYSVRIIPEQFPVVNKSDDIFKQLDLTINSNQVDLNLLDKIENIAK
                     IRDAFRQTDLIVSSLVIKPVEEKELGVEEIVQEEDKKPVAPEAKAVTTAGVKPPADDG
                     GGAEAVSSDQAQVLEDQAQEAERLKKSFAGDSIESQALRRATNHEFSWIYNHVLFDLM
                     GRHGIMPDPALTNQLAILVKNHLTTDLTPDQIRNLFANPALRLAEVRKIYEKLASQPE
                     FVQKLRGQYMAHLDSSTEQQKIAFLESTGFDALDDAKAILKKRKDQLESNTSQIEAFK
                     QSPVKQAENSLATITGITVDGANAVNFERAVVAVTQAMGSKDIARDLGLQYPENKADL
                     TTFVKKLSAEQLVLIFFANPNNPNAIEGSTLTAVSQNIEKIKALLTTALLATSIDKVV
                     DSATELKKLLGVLESTEANPSDAEVESLVTSVQTLTGTMPVGNKRDEVSEALTTDERK
                     SHTQELMDQYQKRVQKIWLQLDEETQLQAYYKLYNMVPNEANRAVLLDKVEHLRDVET
                     GSLPWNPNLFNLIKWEDFLRHSAKQQSERKAGEKKVDFEKLTQELKLTPEEIASSKNA
                     TVDKLRETSAGGFIKNLSGQQVEQLQFSHLITELEKGIAYAQGVSDKIVKEEDVSDLE
                     YRSALGVLERVDTRLILLKKQAPLGDDKSFDQSLQDKILELSDQHHFVLQTLLAYQPR
                     LNKRVGATEDESSIVAPNTEGRFPQYKRLSLVETYQVSVEYQAPEILLPSYVILQQAA
                     LNPEAEMADGVSAETLLADPNSLSLASLNQAATKQNNLTGNNKHKIAAGQQSNQALAD
                     AKALVGDIGQIASQSKGNWVAAAGLGIKKYFTDKEFRQRINRVLMKIAQIGMAGFIAG
                     TALVGLLLQKLIGTVGAWGGAAAGAAAGAGFGFLIGGPIGAVIGGVGGGIAGYLAGGK
                     LAALNAASGAELLSPGLTSPIGFEGAGSVLPSSTAGAGATVGTPLTTSYGSSGGSASA
                     TVGSSQAASQSAFQSQALNSTAPSTTGVTSAAQAAQTSQLGASGTAAAGEAAATSGAT
                     AAAAAQAPFLGSLAGLPILALIPGGSMFILAMITVYTLFVIFSAFLIPLPVGNLSGTN
                     STQSEYATLTKRAAPTEIADGSTATVIYKVTLQSRRNYRLKVTAVTDTANTGFTFQSS
                     RPTHVDPQVAPPATLLNPRISLADFSPDFDNQTQEKEYSVIFNGGKQVLVSNTVTVTY
                     DVQDAQGNVVATGETLSATANVNIGDHGVACWPASGEIWQLPGGSFSHRPSNGLVLDA
                     YDIGAVQGTNIYTPFDGWAHTGDFPPRRSYGKFVVVDSFVRGHWIRLIYGHFMSTTIT
                     NRASDAFTNVGINHDGDGGANDGSNNVGQRVTAGQLLGKMDDTGYSFGSHLHYELIFI
                     DGVRHSLDPSDPLTLEDLVPRPSSGQVREGDNVRTCYN"
     gene            6418..>6712
                     /locus_tag="PKH60_03930"
     CDS             6418..>6712
                     /locus_tag="PKH60_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17221.1"
                     /translation="MFALVTTKLKSLSKRTILFYLLTLVTMVGVVFKLLTPNQINPTT
                     NQNYDGSLSRFSSIKFTGRNITIPDQLPAYTSVEWRSPEQLQAQFLNSFSVEAD"
BASE COUNT         1955 a         1556 c         1398 g         1803 t
ORIGIN      
        1 gactctccca tattgatggc aggagtagcg gaaccaccta ataacttttt tggaagtaat
       61 ttgcccaaac gcctctgtct ttcaattttt tcttcggcgg ttaaaatggc tacaacatcc
      121 gtcggttttg gaataataac tctaccgctt actatctcat cctgtaggat tcgagccacc
      181 tctgctttct ccccttcgtt tgcatataca tacctcctat tgtaggtgct atatagtcgc
      241 tgcgaatgaa acacttgttc gccaaatgaa gtttcggatt gtttcccatc cttatcaagc
      301 aacttaggta actcttgaac aactctccag tcggaccccg ttggcaactg actgctaaaa
      361 aatgaaccaa acttttcata tgatccaggt actggttgct cggctataac ggccttccac
      421 tcactacccg caagatatgt ttttcgacaa agctccagct tctctttgaa ctccttctga
      481 atatcgggaa ttggtatggt atcaaataat ttgctataat tgagagtctg gttttttgta
      541 taaatgggtg ccgcagaaaa attagcctca gtctcagctg tgtgtcgatc catcggatct
      601 ttccagagat aatgagcttc atagctaaca tattctgaaa atattctgtc gcaaaccatt
      661 agtacctcag ccctgctctc gcccctacta tctggagtgg tcaaaacatc ggggtatttt
      721 gttctaacga atgatgacca aaaatcggtt gggtcgatct cgtctttctt gatcgatgct
      781 acatcagcag agctttcaac gggtgttact ccgctgattt tttcctcaat tattaccatt
      841 ctctttgctt tttctatttc aactaaatca aatatctctt tgggtagcaa tttctgaacc
      901 tgcaccaact tgcggaaaag tggcattact gtttgtctaa cgatattctg agttattttt
      961 ctcaaagttt catcggatgt cattttttcc attgctactt catcttcaat agcattgata
     1021 gcaacgtcga tgagatccaa ctgctttaga acttcgtctg gaagtattaa aacctcccct
     1081 tcaaccaaag gtgactcagc ttcagccacc gatggtaacc caacctcagc aacctctggc
     1141 agaactgttt ctactgctgt tgctgagtga tctgattcct gaatatcaaa acgttctagt
     1201 aaaatttgct taaaccttgt tggaagtttt acctccttat taaggtgaag aagtatgtcg
     1261 acaagtcttc tttttgattc ttccggagat ttccccccat caggtctcat ttcatcggca
     1321 agagctttag agaaatcggc caatgtatat gctccttttg gttctgtgtc tatcattagt
     1381 ttctcccttg tggggtatta tatcaatatc ttaccaagat taacaggagt taacatatta
     1441 aacgtattgc caccatgatt gtatttcctg ttatgttttg atacattcta ttgatgtcgg
     1501 aacgcgtgac aattccccca aactttgttg accacattat gggtggtgat atttgcccag
     1561 aattgttgtt gctcatgttg agtatccagc gggattttga tgaacagcag gtaactctgg
     1621 aaaggcgtct actcaactta tctgcttgtg aatgcgatcg ttgttgggat ttgtattggg
     1681 ccagatggaa tgagctcaga tccagagatg acaaccttca ggacttacaa gaagacgaga
     1741 gcgtgcttga agatatacat cgctatgccc ctaaacttga ggaagttgac taagtttttt
     1801 atccctccgc agggacttca taaccagcta taatcaaagg catggctgat acacagaccg
     1861 gcgttggtgt agtggattta ccaccaaatg taatcaattt ggccgatatc aaacgactac
     1921 aattgaactc gttagaggat gtaattaatg ccaccaagtt gctctctaga gtagttgcag
     1981 aaaaaaaaga agagttatta tttgaaaaac aatcagtcac cctaaaacta accagtggcg
     2041 tattttctcc acaaactact aacaagtggt gggaacaaga ggtagttgat aatccaaaca
     2101 atttcctact tacaacagat tttaccaatt tcattgcgca aattgaccta tggcttgtac
     2161 aacacgctga agatattcaa aaaaaatatt ctgttcgaat aattccagaa caattccccg
     2221 tagtcaataa atctgacgat atttttaaac aactggattt aacaatcaat tcaaatcagg
     2281 ttgacttgaa tttactggac aagattgaaa acattgccaa aatccgggac gcctttcggc
     2341 aaaccgatct catcgttagc tcgctggtta ttaaaccagt tgaagaaaaa gagctgggtg
     2401 ttgaagaaat cgtacaggaa gaggataaaa aacccgtcgc tccagaagca aaagcagtca
     2461 ccactgcggg agtgaaaccg ccagctgatg atggtggtgg cgcagaggct gtatcctctg
     2521 accaagcgca ggtactcgaa gatcaagcgc aagaagcaga gcgattgaaa aaatcttttg
     2581 ctggtgactc cattgaatct caggcgttac gacgcgccac caatcatgag ttctcttgga
     2641 tttacaacca cgtgttgttc gacctgatgg gtagacatgg cattatgcca gatccagccc
     2701 ttaccaatca gcttgcgata ctagttaaaa atcacctgac tacagattta actccagatc
     2761 aaatacgaaa tttatttgcc aatcccgcac tacgtcttgc tgaagtcaga aagatttacg
     2821 agaaactagc tagtcaacca gagtttgttc aaaaactcag aggccaatac atggcccacc
     2881 ttgactcctc caccgaacaa caaaaaatag cgtttttgga aagcactggt tttgatgctc
     2941 tcgacgatgc aaaagcaatt ttaaaaaaac gtaaagacca gctagaatca aacacctctc
     3001 aaattgaagc ttttaagcag agtccagtca aacaagctga gaattcatta gcaaccatta
     3061 ccggcatcac agtcgatggt gcgaatgccg ttaattttga acgagcagtt gttgcggtaa
     3121 cccaagccat gggttcaaaa gatattgcgc gagatcttgg tttgcaatat cccgaaaata
     3181 aggccgacct caccacgttt gtgaaaaaat tgagtgctga gcagctcgta ctcatctttt
     3241 ttgctaatcc caataatccc aacgcaattg aaggatctac tcttacagca gtctctcaaa
     3301 atattgaaaa aatcaaggcg cttttaacca ccgcgctttt agccacatca attgacaaag
     3361 tagttgactc tgccactgaa ctcaaaaagc tcttgggagt attggagtca actgaggcaa
     3421 acccctctga cgcagaagtt gagtcgctgg taactagcgt gcaaactctt actgggacaa
     3481 tgccggttgg caacaagcga gatgaagtta gcgaagcact caccactgat gaacgaaaaa
     3541 gtcataccca agaattgatg gaccaatacc aaaaaagagt acaaaagatt tggctccaac
     3601 ttgatgaaga aactcaacta caagcatact acaagctgta taacatggtt ccaaatgaag
     3661 ctaatagagc cgtgcttctg gataaagttg agcacctgcg agatgtagaa acgggcagtc
     3721 tcccatggaa tccaaatctg tttaacctaa ttaaatggga agattttcta agacactcgg
     3781 caaaacagca atctgaacga aaagctggag aaaaaaaggt cgattttgaa aaattaactc
     3841 aagaactcaa attaacacca gaagaaatcg cctcaagtaa aaatgccact gtagacaaac
     3901 tgcgagaaac ttccgccggt gggtttatta aaaatttaag cggacaacag gtagaacaac
     3961 tacaatttag tcacctgatc accgagttag aaaaaggcat cgcctacgcg caaggtgtgt
     4021 cagacaagat agtcaaagag gaagatgtta gcgatcttga ataccgctca gccctaggag
     4081 tattagagcg cgtggatact cgcctaattt tacttaaaaa acaagctccg ctgggtgacg
     4141 acaagtcgtt tgaccaatct ctccaagaca aaatcttaga actctcagac caacaccatt
     4201 ttgttctaca aacgctcctt gcttaccaac caaggctcaa caagcgagtt ggtgcaacag
     4261 aagatgaatc ttctattgtt gccccgaata cagagggtcg cttccctcaa tacaagaggc
     4321 taagtctggt ggaaacctac caagttagtg tggaatatca ggcgccagag attttgcttc
     4381 catcatatgt gatattacaa caagcagcac tcaatccaga agcagagatg gctgatggcg
     4441 tttctgctga aaccttatta gctgacccca actcgctttc tttggcatcg ctaaatcaag
     4501 cagctaccaa acaaaataac ctcacgggta ataataaaca caaaattgca gcaggccagc
     4561 aatcaaatca ggcactagcc gatgccaaag cattggtggg cgacattggt caaatagcat
     4621 cacaatcaaa aggcaactgg gtggctgcgg caggattggg gataaagaaa tacttcactg
     4681 ataaggaatt tcgccaacgt atcaatcgag ttttaatgaa aatcgcccaa attggcatgg
     4741 cgggctttat tgctggaaca gcgctggttg gcctactcct ccaaaaactc attggtacag
     4801 ttggagcgtg gggaggtgct gcagctgggg ctgcggcagg agcaggtttt ggctttttaa
     4861 taggtggccc aattggagca gtgattggtg gggttggggg cggtatagcg ggatacttag
     4921 ctggaggaaa gctagcggct ctcaacgccg cttctggagc agaactatta tcccccggac
     4981 taacatctcc aattggtttt gagggtgcgg gttcggtatt gccatcatca acagctggag
     5041 ctggtgcaac agtaggaacc ccacttacta cctcatacgg tagttctggt ggatcagcct
     5101 cagcaactgt cggttcatcc caagcggcgt ctcaaagtgc tttccaaagc caggccctaa
     5161 attcaactgc cccctcaaca acgggggtta cttcagccgc ccaagctgcc cagacttctc
     5221 agttgggagc atcgggcaca gctgccgctg gagaggcggc ggctacatct ggcgctaccg
     5281 ccgctgcagc tgcacaagct cctttcttgg gcagtcttgc cggtttgccc atcttggcat
     5341 taataccagg cggatcaatg tttattttag ccatgattac tgtgtatacc ctgtttgtga
     5401 ttttttctgc atttcttatt ccacttcctg tgggcaattt gagcggcact aatagcacgc
     5461 agtctgaata tgccacactc acaaaacgag ctgctcccac agaaatagca gatggttcca
     5521 ccgccaccgt catttacaaa gtcacacttc aatcaaggag aaactatcgt cttaaagtga
     5581 ctgcggtgac tgataccgcc aacactggtt ttacgtttca atctagtcgc cccactcacg
     5641 ttgatcccca agtcgcaccc cccgcaacac tgcttaaccc tcgcatttcg ctggcagatt
     5701 tttcaccaga ttttgataac caaacacaag agaaagagta ctccgttatc tttaatggag
     5761 gtaaacaagt gcttgtttcc aacaccgtta cagttactta cgacgttcaa gacgcccagg
     5821 gcaacgtagt cgcaacaggc gaaactctca gcgccactgc caacgttaac attggcgacc
     5881 acggagtggc ttgttggcca gcatctggtg aaatctggca attgcctggg ggaagttttt
     5941 cacaccgacc cagcaacggt ctagttctag acgcatatga tattggcgca gttcaaggaa
     6001 ccaatattta taccccattt gatggctggg cgcacaccgg agacttccct cccagaagaa
     6061 gttatggtaa atttgtggtt gttgactcgt ttgttcgggg acactggata cggttaattt
     6121 atggtcactt tatgtctaca accatcacca atcgcgcaag cgacgccttt accaacgttg
     6181 gaattaatca tgatggcgac ggtggagcaa atgatggaag caacaatgtt ggccagcgag
     6241 taactgctgg tcaacttttg gggaaaatgg atgacaccgg ctactcattt ggctcacacc
     6301 tccattatga attaattttt atcgacggtg ttcgccactc tctagaccca agcgacccac
     6361 ttaccttaga agacttagta ccaagaccct catcgggtca agtcagggag ggagacaatg
     6421 ttcgcacttg ttacaactaa attaaaaagt ttgtcaaaaa gaaccatcct cttttatctc
     6481 cttactctag ttactatggt gggtgtggta tttaaattgc tcacccccaa tcaaataaac
     6541 ccaacaacta atcaaaatta tgatggttct ctctcacggt tttcatcaat caaatttacc
     6601 ggaagaaata ttaccattcc agaccaacta cctgcctaca cctcagttga gtggcgaagt
     6661 ccagaacaac tacaagctca gtttctcaat tccttttctg tagaagcaga tg
//
LOCUS       DAOQZP010000047         6269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_12802_length_6269_cov_4.845188, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000047 DAOQZP010000000
VERSION     DAOQZP010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12802_length_6269_cov_4.845188"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(515..1282)
                     /locus_tag="PKH60_03935"
     CDS             complement(515..1282)
                     /locus_tag="PKH60_03935"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNQ17222.1"
                     /translation="MPNLGRELRAFVDFHRNIMSDWIDKLISLDLSVTESDDEKSDSE
                     KTKQPSIPVITINKPNQEQLWFTSTETKQSFHHTIIDKQGNSSDLLIEVFHQFCREDY
                     YSVVSRVLAIHEQGEHSAANTYLLLSSETLEQAIQPTSLVIIANIDAEPVAFALLNEI
                     PNDHDHVSEILIEVVPHKRNSGIATALFYATLQHPNTLPSHIEAEVRTMNKPVFSLLK
                     KAQDEGYIRYTLKVSGPETLAEIEILNTSEASAGSLL"
     gene            1325..1570
                     /locus_tag="PKH60_03940"
     CDS             1325..1570
                     /locus_tag="PKH60_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17223.1"
                     /translation="MRALVRSVLPPSQRSALSTFGQNRAMVKAKRLDKLGAERRKMAA
                     AQVGAEPSLTPIFKQRSDLLERQIGRLQKKDEHDSPT"
     gene            1615..>2315
                     /locus_tag="PKH60_03945"
     CDS             1615..>2315
                     /locus_tag="PKH60_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HNQ17224.1"
                     /translation="MPEQTISCPHCGQLIPLTQALTHQIESVALAKVERKKAELEKAQ
                     QQLEVERRAQTQLVEEQVKKQLETEKRQLWARAQSEAAKQQDTKTKDLEAQVAEKDKK
                     LAEAQQQELELRKRQRQLEEKEQALELETQRKIDQEREKIRLETQKQEAERSQLSLQE
                     KDKQLDIMRKTIEDLRRQGSQGSQQVQGEVGEDWLKQTLNDNFIQDLVEDVATGKLGG
                     DILQRIQGRTGRTVAT"
     assembly_gap    2316..2415
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2416..5121
                     /locus_tag="PKH60_03950"
     CDS             <2416..5121
                     /locus_tag="PKH60_03950"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNQ17225.1"
                     /translation="ERFSLFQQILDSLDLELLKPLNRTDYYISDIIKAISDLKKEGVS
                     PEQFAEVLLTAWPEADSAPSKTVRMQQAKNRAKNLELAKFYQAYEVQLRESLRYDFDD
                     MVALVVQVFAENEMVRLQYQEKFQYVLVDEYQDTNSAQNQVVDQLMSFWQEQPNLFVV
                     GDPHQSIFRFQGASLANTASFISRYPDAKLIVLTQGYRCSQTIYDVAHALISDQSNQE
                     LADLNPSLSTVLELANKQPLCSVHDKQSNIQIISAPSQLSEYLWISQQVVQHVAAGVA
                     PQDIAVLFRTNQEIQAFEAVFAHHDVPYETDAGEDILTVEPILQLIKLCSVLQEIRNG
                     EIHPDIFTVLSYEWLEVPYLTLLKLARMAHARKTTFFELLINQPKFSSEINKESITVL
                     EWQAIADVFSKMLTWINKQDNLTFTQWIELVLQESGYLSWLIKQPKKIEYLLAVNTLY
                     EEIKAQVKSNHSFGLTEFLQSITTMQQHRIKIVRAPLLLEKNAVRLSTVHKAKGQEWQ
                     IVFIPGLIDGKWGNSKKRQLLPLPQELIPNATITPEETNAEERRLFYVALTRAKTNVY
                     LSYSESVVNENRSKSVNQSEFLVALLEQQSSNFKTLLATDSLPKLDAQLERLLLPANS
                     QRKLQSQEREYYKYLVSNFSLSVSSLNKYLRSPENFALDVLLRLPKAKEPAMAFGTAV
                     HTALEHFYKQSIQLKTLPQLPTLQTAFLNAIEQEVLTESEFTARKKHGIKILENYYHN
                     LPKELPKIVSLETFFGHGQHPTYLQDIHLSGRVDRIDVIPNSKNQVRVIDYKTGKPKS
                     PRAILGLSQIDRYSPRELALPEPIRGEYKRQLLFYKLLADVSPNFTSQVKEGVFEFVE
                     PNTSGKIIKHQFELHDEEVELLKQLIIDVMAEIRGLAFVSENDIK"
     gene            5161..>6269
                     /locus_tag="PKH60_03955"
     CDS             5161..>6269
                     /locus_tag="PKH60_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17226.1"
                     /translation="MLQNIHESLIPYQSQMRPELETLFETINRLLLVLESPSNGIEIP
                     SISEISDSNWTETALREFLNVSEADSGRFLANTQMGNYPWVLGVPTYLDIENPELRKH
                     VLAKELTTELSWWHPISMTRINNFVLNAARVANVPKEEVDRIANLMTREAYRSRQPGL
                     EKERVSPPEFEFEKSFSVSQLLSVLYEDIAERWKKLELFQKMFPTKKGDMYIRRTGEM
                     SPEEAASLERFIRITPMGQKNEVVMEFNLWEVIDHRLTTAVMELDSIAQRISQARPME
                     LILDLVKFSKEHPIDREISSILGSFYYYDENYLPREKALGQELEVNDGQLSKDRTKNN
                     VLRIRPDLYVEIPGGITMGPDSLAGLVMQEKDYFAL"
BASE COUNT         1987 a         1136 c         1330 g         1716 t
ORIGIN      
        1 taattttgat tcgacttgtg aacgaaccaa cgcagctttt aattcaggtt ttttaacccg
       61 attaatcagg attgaaagca gaaaatctgc agaggttgta ggtaactact caagaccttc
      121 aagacattct ggttcaacgc gtttgacgtt ttctaaaatt gcttgcaatg tcttgtcaat
      181 gtactccggc tgtgtggtgg caggttcaaa catatcttta agtatatcta agctccatta
      241 agaagtgtag tagagaaaga gaaagccccc gcgggtgcgg gggctttggc gcccgggtca
      301 gctgtcgtga cggctggccc ggggtcaata gaaatcaaaa actttaaaca ttggagcgcc
      361 tcctggaaaa ccccccaacg ggtcgacgtt ccccaaaaaa gctccttcct gatttctaga
      421 ttttgttgac ctctgctcac gaacaagctc gcgagtttca atcaacaata tagatactat
      481 aacacaatca ttacaaatag tcaaatacta tgggttataa taatgatcca gcgcttgctt
      541 ctgatgtatt aagaatttca atttcagcca gagtttctgg accgcttact tttagggtat
      601 acctaatata accttcatct tgtgcttttt taagtaaact aaatactggt ttgttcatag
      661 ttctcacttc ggcttcaata tgtgagggta gcgtatttgg atgttggagg gtggcataaa
      721 acaaggcagt ggcaatgcca gaatttcgtt tgtgaggcac tacttcaatt aaaatttcac
      781 tgacatgatc gtgatcattt ggaatttcat ttaaaagagc gaatgctact ggctcagcat
      841 caatgttggc aataattact agactagttg gttggatcgc ttgctcaaga gtttccgaac
      901 tgagaagtaa gtaggtattg gctgcagagt gctcaccttg ttcgtgaatt gcaagcacgc
      961 gactaacaac tgaatagtag tcttcacgac aaaattgatg aaacacctct attaataaat
     1021 ctgatgaatt cccctgttta tcaattatcg tgtgatggaa agattgtttg gtttcagtgg
     1081 acgtaaacca gagttgttct tgatttggtt tgttaatggt aattacagga attgatggtt
     1141 gctttgtctt ttcagaatca gatttttcat catcgctttc cgttactgat aaatcaagac
     1201 ttattaattt atcaatccaa tcactcatta tgtttcgatg aaaatcaaca aaagccctaa
     1261 gttctcttcc aagatttggc attgtaaaga ggtattttac tcctcaattt gatatactgg
     1321 tactatgaga gctctagtgc gaagcgtatt accaccaagt caaagaagcg ccttatctac
     1381 ctttggtcaa aatcgcgcaa tggttaaggc gaaaagattg gataaattgg gtgcagaacg
     1441 acgaaaaatg gcggcggcac aagttggagc tgaacctagt ttaaccccaa tatttaagca
     1501 acgaagcgac ttattagaac gccagattgg gcgtttacag aaaaaagatg agcatgattc
     1561 tccaacctaa ttaaagcttg acttgtaacg gttggacacc tagagtatcg ccatatgcct
     1621 gagcaaacca tttcttgccc tcattgtggt caactaattc ctttaactca agccttgact
     1681 caccaaattg aatctgttgc tttggccaag gtcgagcgaa aaaaagccga gttggaaaag
     1741 gcgcaacagc agttggaagt ggagaggcgc gcgcagacgc agttggtgga agaacaagtt
     1801 aaaaaacaat tggaaaccga gaaacgtcag ctttgggcgc gcgcgcaatc cgaagccgcc
     1861 aagcaacaag acactaaaac caaagatctt gaagcccaag tggctgaaaa ggataagaaa
     1921 ctagctgaag cccaacaaca agagctagaa cttcgcaagc gccaacgtca attggaagaa
     1981 aaagagcaag cgttggaact tgagactcag cgcaaaattg atcaagaacg agaaaaaatc
     2041 aggttggaaa cgcaaaaaca agaggctgag cgcagtcagt tgagtctcca agaaaaagac
     2101 aagcaactag atattatgcg caaaactatt gaagacttgc gtcgccaggg ttctcagggt
     2161 tcccagcaag ttcagggcga agtcggtgaa gattggctta aacagacgtt aaatgataat
     2221 tttattcaag atttagtaga agatgttgct actggcaaat tgggtggtga catcttacaa
     2281 cgaattcagg gtcggacagg tagaactgtg gccacnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnngagcg gttttcatta tttcaacaaa ttttagattc gctcgatcta
     2461 gaattactta agccacttaa tcgaacagat tattatattt ccgacattat aaaagctatt
     2521 tctgatttga aaaaagaggg tgttagccca gaacagtttg ccgaagtatt gctcaccgct
     2581 tggcccgaag ctgattccgc tccaagtaaa accgtgagaa tgcagcaggc aaagaatcga
     2641 gccaaaaact tagagctggc aaaattctac caagcctatg aagtccagtt gcgtgagagt
     2701 ttgcgctacg attttgacga catggtggct ttagtggttc aagtatttgc tgaaaatgaa
     2761 atggtgaggt tgcagtatca agaaaaattt caatatgtgc tggttgacga atatcaagat
     2821 accaactcag cccaaaatca agtggttgat caactaatga gtttttggca ggaacaaccc
     2881 aacttgtttg tggtgggcga tcctcatcaa tcaatttttc gatttcaagg agcatctctt
     2941 gccaatacgg cctcatttat tagtcggtat ccagacgcta aattaattgt gttgacgcag
     3001 gggtatcgct gttctcaaac catttacgat gtggcccacg cgttgatttc agatcagtct
     3061 aaccaagaat tggctgatct taatccatca ttgagtactg tattggagtt agccaacaag
     3121 cagcccctgt gtagtgtgca tgataagcag tccaatatac aaataatttc tgctccatcg
     3181 caactgagtg agtatctttg gataagtcag caagttgttc aacacgtggc agcgggagtg
     3241 gcgccacagg atattgctgt tttatttcga actaatcaag aaattcaagc atttgaagcg
     3301 gtatttgctc atcacgacgt tccatatgag actgatgctg gtgaggatat cttgactgta
     3361 gaacctattt tgcagttaat aaaactttgt tcggtgctac aagaaattag aaacggtgaa
     3421 attcatcctg acatatttac agtcttgtca tacgaatggt tggaagtacc atatttgact
     3481 ttgttgaagt tggcgcgcat ggcacatgcg cgcaaaacca cattttttga acttttaatt
     3541 aatcaaccaa aattttcaag cgaaataaac aaggaaagta tcactgtatt agagtggcag
     3601 gctattgctg atgtttttag taaaatgtta acttggatta acaagcagga taatttgacg
     3661 tttactcagt ggattgaact cgtattgcaa gaatcaggct atttatcttg gttgatcaaa
     3721 caaccaaaaa aaattgagta tcttttggca gtgaacacgc tgtatgaaga aatcaaagca
     3781 caggtaaaat ctaatcactc atttggacta accgaatttt tacaatcgat tactacgatg
     3841 cagcaacata gaataaaaat tgtcagagca ccgttgttgc tggaaaaaaa tgccgttcgt
     3901 ttatccactg tccataaagc gaaagggcaa gagtggcaga tagtctttat tcctggcttg
     3961 attgatggca aatggggtaa tagtaaaaaa agacaactac tgccattgcc tcaggaatta
     4021 attcccaatg caacgattac gccagaagaa acaaacgctg aagaacgacg attattttat
     4081 gttgcattaa caagagcaaa aaccaacgta tatttgagtt attcggaaag cgttgttaat
     4141 gaaaatcgga gcaaatcggt taatcaatcg gaatttttgg tggcactgct ggagcaacaa
     4201 tccagcaatt ttaaaacact gttagctacc gattcattac caaaattgga tgcacaacta
     4261 gagcgattgt tgttaccggc taattcgcaa agaaaactcc aaagtcagga aagagaatac
     4321 tataaatatc ttgtgtctaa ttttagtttg tctgtttctt cattgaataa atatctgaga
     4381 agcccagaaa attttgcgct tgatgtttta ttgcgactgc caaaagcaaa agaaccagct
     4441 atggcctttg gcactgcggt ccatactgca ctagagcact tctataagca aagtattcaa
     4501 ttgaaaacct tgccacaact tccaacttta caaaccgcct tcttgaacgc catagaacaa
     4561 gaagttttaa cagagagtga gtttactgct cggaagaagc atggcattaa gattttagaa
     4621 aactattatc acaatttgcc aaaggaacta cccaaaattg tttctctcga aacatttttt
     4681 ggtcatggtc agcacccaac ctatttacag gatattcacc taagcggaag ggtggacaga
     4741 attgatgtaa tacctaattc aaaaaatcaa gttagagtaa ttgactataa aacaggtaaa
     4801 ccgaagagcc ctagggcaat tttgggactt tcccaaattg ataggtatag tccaagagaa
     4861 ctggccctac cagagccaat tcgtggtgaa tataagcgtc aacttctgtt ttataaactg
     4921 ttggctgatg tatcaccaaa ttttacctcc caagtgaagg agggtgtctt tgaatttgtt
     4981 gaaccaaata ccagtgggaa aataattaaa catcaattcg agctacatga tgaggaggtg
     5041 gagctgctaa agcaattaat tatcgatgta atggctgaaa tcagaggttt ggcttttgtt
     5101 agcgagaatg acataaagtg atatactatt actgtagctg cccattgagt gtggaggagt
     5161 atgttgcaaa acatccatga atctttgatt ccgtatcaaa gtcaaatgag gccagagcta
     5221 gaaacgttat ttgagaccat caacagattg ttgcttgttc ttgaatcacc ttctaatgga
     5281 atagagatac cttcaatttc ggaaatttca gatagcaatt ggacagagac ggcattgaga
     5341 gaatttttaa atgtaagtga ggcagactcg gggcgttttc ttgcgaatac tcagatgggt
     5401 aattaccctt gggttttggg agttcccacc tatttagata tagaaaatcc cgaacttcgc
     5461 aagcatgtat tggcaaagga attaactaca gaacttagtt ggtggcatcc aatttctatg
     5521 acaagaatta acaactttgt tcttaatgca gcaagagttg caaatgttcc aaaagaagaa
     5581 gtggatagga tcgcaaatct aatgacacga gaagcatatc gctctagaca accagggttg
     5641 gaaaaagaaa gagtatctcc tccagagttt gaatttgaaa aatcattttc ggtttcacag
     5701 ttgcttagtg ttctctatga agacattgca gaaagatgga aaaagctcga gttgtttcaa
     5761 aaaatgtttc ctacaaaaaa gggtgacatg tatatcagaa gaacaggaga gatgtcacca
     5821 gaagaagctg catcacttga gcgatttatc cgcataaccc caatgggtca aaaaaatgaa
     5881 gtagttatgg aatttaattt gtgggaggtg attgatcatc gcttaactac cgcagttatg
     5941 gagctagata gcattgcgca gcgaatttcg caagctagac caatggaact gattctagac
     6001 ttagtaaagt tctcaaaaga acatccaatc gatcgagaaa tttccagtat tttgggaagt
     6061 ttttactatt atgatgaaaa ctacttgcca agggagaagg cattggggca agagttagaa
     6121 gtgaacgatg gtcaactatc aaaagataga accaaaaata atgtattgag aattaggcca
     6181 gacctctacg tagaaatacc aggaggtata actatgggtc cagattcact ggctggttta
     6241 gtaatgcaag aaaaagatta ctttgcact
//
LOCUS       DAOQZP010000048         6209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_12946_length_6209_cov_5.888528, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000048 DAOQZP010000000
VERSION     DAOQZP010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6209
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12946_length_6209_cov_5.888528"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..298
                     /locus_tag="PKH60_03960"
     CDS             65..298
                     /locus_tag="PKH60_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17227.1"
                     /translation="MFEHKHGYKYEIEAQRNKPYIIQQLEIIRKTPPESFQANPWLFF
                     TLTAPKPGFVRLYAGVAPTALNGFLPRHLPKKP"
     gene            358..612
                     /locus_tag="PKH60_03965"
     CDS             358..612
                     /locus_tag="PKH60_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17228.1"
                     /translation="MAAKRIYQPDQWLTSSFENARQEHARGGFVLSVDLLINDVSKYI
                     ELFTVRTNEIVYNVPREIVKQSLVQIAAFGPNSLQYFQSH"
     gene            complement(613..2889)
                     /locus_tag="PKH60_03970"
     CDS             complement(613..2889)
                     /locus_tag="PKH60_03970"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HNQ17229.1"
                     /translation="MIAGQEVPKSFIQRAREGRLFDPATGHQISESRAAEILQDLHYQ
                     LTRTDGKAIGEDQVAAYLQDFTELPITATQQHQATLEGKTLGMDNTELVKELRSVMTQ
                     VEIELAAGNLQPSNVETYIQQTFPTFKYDLKVIQDVFIDRYRKFANYCEHSTSIEQLM
                     LSLENSRARFSPNYTNDQVIGVINRLVADARSGKMNEGSVSKSLVTGGFIPNKLDNPI
                     YLALHRLEQSDPQIQSQRQQSNEVAQGVDNSQVEAPVETANYSLSLSIISANSVVVET
                     DPRTSAEMVRLMGPDGTNYVRIPLSQLQAAFTVMESITYGMRVGSAHDIDSLMLKHDF
                     NTGKPSHLLVGGKDISQNNTLYGLSISGEALTKVLDIIKPTDLSIEGQPTSIVETMKS
                     KAMKLAQSIQDFLVGHNSKDEVTQNETSVEIPKPGISTLKAPEHFNQDRSGSADVKYE
                     VTDDAGNKVLAEGIRIVVNDGSGGSLPEEITEDKGRSNLIGNKAKVGLDAILQASNLS
                     PAEAVRAAHRKIKDTNESYSTVMVADVVIENGKGKLRFAGLGDPSVVAYRQDKPFEHR
                     RSELSGAFVSNSMDAAERARILADPSHMQIQNLQHNGALLDKIYGEGRKVKTAALDAT
                     LRANGIYLNFGDKKRSDVHPVNFTVDLEELDADFILVMSDGAQPNSMLATDPKVDLSD
                     DIAAEEVDNPDGAESMRNQPRYAQEVQSIMSELTAGTIDVKTASERITSAARNTLGDK
                     DDITVAIIDVRQFRKKTN"
     gene            complement(2970..3281)
                     /locus_tag="PKH60_03975"
     CDS             complement(2970..3281)
                     /locus_tag="PKH60_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17230.1"
                     /translation="MQLTKTQLFEEMRNQGLDEETITNLMTDFADLPEELSQTDLERV
                     DSVLAEVQGAHGFAAFTHGEMADALENAADKILDAADDYIEESAKSTVNHVQLAQSML
                     E"
     gene            complement(3310..4404)
                     /locus_tag="PKH60_03980"
     CDS             complement(3310..4404)
                     /locus_tag="PKH60_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17231.1"
                     /translation="MTENGNPVVGLEAQLANSAGAEVAAGAGPEDLTKVTLKRPEDPA
                     LVRLLENKLREYNQRIAERLKDDAYQAPEEADFDLRYRVDILDNVLGLRNGESISVDQ
                     AINSVRAKHGGVFNPDQFNNAFGVVRNYIEHAGQGNDGGTGLSEYGSAKARNIAEAAS
                     KIKGAAEQRAQERQDRITQRKGRWGERFAKFKDAGKKAWEKVGQLKDAAGNKAKDAVR
                     ETGQMIVDSPIVAKVDLIQAGARAEAAKLGAKLVDTADRVTTGVAEKIDATGMKVEEY
                     KAASKAKAAEVVVDSLRGAVSKVRAGMDAVDKQLMKIDAAGEVAEDGPTPIVNRLEEK
                     AQEQRQKQEDATKRERSVRGLRGWLRRLGA"
     gene            complement(4412..4951)
                     /locus_tag="PKH60_03985"
     CDS             complement(4412..4951)
                     /locus_tag="PKH60_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17232.1"
                     /translation="MKLQTILDQLSLDIEQYIEEEVVRRTNTTNSQFGVSQQPVSKPV
                     SVEEDNFQSALVVLKKLLDSKKSTRHSFSADQELSNLRDLTDLTANKRFDVVPTAAQT
                     NQQMEDYLQILIELATKPNPSQNESVFLEELSSLIDKTAIQTGVQDQSVVTTKSSNAF
                     DGLKMFLRTIGDRNSNTTT"
     gene            complement(5006..>6209)
                     /locus_tag="PKH60_03990"
     CDS             complement(5006..>6209)
                     /locus_tag="PKH60_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase"
                     /protein_id="HNQ17233.1"
                     /translation="KVLNLILEASYHDGTGEPGFINQHRLVQNDEGYAGYQDGKFAEG
                     LKYKPLTRTEKMLARIARNAEAKVYQQIPNPCGEISLNMLGGYCVIADVVPYYCPTVD
                     DAEEAYRAATRALIRVNTMDCLYNREVKRTNRIGVGMTGIHEFGWNMFGYSFRDLIDE
                     TKSKDFWLTIARFKRAVVDEAEQYSKKLGVTVPHTNTTIKPSGTISKLFALTEGAHLP
                     SMREYIRWVQYRSDDPQVKKYKDQGYPIKELRSYEGSTAVGFPTQPLICRIGMNGELV
                     TAAEATPEEQYKWLMLLEKYWIVGVDEQGNPLEDTGNQVSYTLKYDPTKVKFAEFTEM
                     VKQYQSQVKCCSVMPQQDSTAYEYQPEQPVTISEFMRVLDEIEKDEELFEDIDWEHLK
                     CETGACPI"
BASE COUNT         1602 a         1476 c         1107 g         2024 t
ORIGIN      
        1 gtaagtattg tgcatccgga tattctatcc gttgcgttaa attgttgaga ccaagaataa
       61 taatatgttt gaacataagc atggatataa atatgagata gaagcccaac gcaataaacc
      121 atacatcatt caacaattag aaatcataag aaaaacacct ccagagtctt ttcaagcaaa
      181 tccatggctt ttttttacac ttactgctcc aaaacctgga tttgtacgct tatacgctgg
      241 agtagcaccc actgccttaa atggtttttt gccgaggcac cttccaaaaa aaccttagca
      301 aaagcagtta aagcaactag ttcaccacac ttacttactg atccaaaagt aagagctttg
      361 gcagcaaaac gaatttacca accagatcaa tggctcacct catcctttga aaatgcaaga
      421 caagaacacg caagaggagg ttttgtgttg tcggtagatt tactaattaa cgatgtttca
      481 aaatatatag agctatttac tgttcgaact aacgaaattg tttacaacgt tcctagagaa
      541 atcgtaaaac aatcactcgt ccaaatcgct gcctttggtc caaatagtct tcaatatttc
      601 caatcgcact gattagttgg ttttctttct aaactgccga acatcgataa tcgcaactgt
      661 aatatcatct ttatccccca aggtgtttct tgctgcgctt gtaattcttt cactggcagt
      721 ttttacatca atagttcccg cagtcagttc actcataatc gattgaacct cttgagcata
      781 tcgaggttga tttctcatgc tttctgcacc atcgggatta tcaacctcct ctgcagcaat
      841 atcatcactc agatctactt ttggatctgt ggctagcatg gagtttggtt gtgcaccatc
      901 agacattact aagataaaat ccgcatctaa ttcctctaga tcaactgtga aattaactgg
      961 gtgaacatcg cttctctttt tatcaccaaa atttaaataa attccatttg ccctcaacgt
     1021 ggcatctaat gcggcagttt ttacttttct accctcgcca tatattttgt caagcaaagc
     1081 tccgttgtgt tggagattct ggatctgcat atgagaggga tcagccaaaa ttcttgctct
     1141 ttcagccgca tccatggaat tgctgacaaa tgcgcctgac aactctgatc ttcgatgctc
     1201 aaaaggttta tcttgccgat atgcaaccac tgaaggatca cctaatccgg caaatcttaa
     1261 ttttcctttt ccattttcta taaccacatc tgccaccata acggtgctat atgattcatt
     1321 tgtatcttta atctttctat gagctgctct aactgcttcc gcaggtgata gatttgatgc
     1381 ctgcaaaatt gcatctaaac caacttttgc tttattacca attaaattac ttcttccttt
     1441 atcttcagtt atttcttcag gtaaagatcc acctgatccg tcattaacta ctatgcgaat
     1501 accctcagcc aagaccttat tccccgcatc atcagttact tcgtacttca catcggcaga
     1561 tccagatcta tcctgattaa aatgttcagg cgcctttaat gtggaaatac ctggtttggg
     1621 aatttctact gaggtttcgt tctgggtaac ttcatcttta gaattatggc caacaagaaa
     1681 gtcctgaata ctctgagcta gtttcattgc ttttgacttc attgtttcca caatagatgt
     1741 gggctgacct tcaattgaca gatctgttgg tttgataatg tctaatactt tagttagtgc
     1801 ctcaccacta attgataaac cgtataaggt attattttgt gaaatatcct ttccccctac
     1861 taagaggtga gagggctttc ctgtattgaa atcatgtttc aacatcagag aatcaatatc
     1921 atgtgctgat ccaactcgca ttccataagt gatgctttcc attacagtaa aagctgcttg
     1981 taactgagaa agaggaattc tgacataatt ggtaccatct ggacccatca atctaaccat
     2041 ttctgccgaa gttctagggt cggtttctac cacaacgcta ttggcactta taattgaaag
     2101 tgagagtgaa tagtttgctg tctccacagg tgcctcaact tgagaattgt ctacaccttg
     2161 agctacttcg ttactttgct gtcgttgcga ctgtatttga ggatctgatt gttctaatcg
     2221 atgtaaggct aagtagatag gattatcaag cttattaggt ataaaaccac ctgttaccaa
     2281 agatttagaa acagatcctt cattcatttt cccacttctg gcgtctgcta ccagtctatt
     2341 tattacacct ataacttgat cattcgtata attcggagaa aatctggctc ttgaattttc
     2401 taatgacagc ataagctgtt caatagatgt actatgttca cagtagtttg caaattttcg
     2461 atacctgtca ataaaaacgt cctgaataac ttttaaatca tatttaaaag ttgggaatgt
     2521 ttgttgtata taggtttcta cattagatgg ttgaagattg ccagccgcca gctcaatctc
     2581 aacttgtgtc ataaccgacc gcaactcttt aactaattcg gtgttatcca ttcccagagt
     2641 ctttccctct aatgtagctt ggtgctgttg tgttgcagta attggaagtt cagtaaaatc
     2701 ttgtaaatac gctgccactt gatcttcacc aattgccttg ccatcagttc gagtcagctg
     2761 atagtgaaga tcttgtaaaa tttcagccgc tcgtgattca gaaatttggt gaccagtagc
     2821 aggatcaaac aacctaccct ctctagctct ctgaataaaa ctttttggta cctcctgacc
     2881 agcaatcata tatttaccat aatactacag agacgaatgc tttgcattcg tctctatgtc
     2941 acttctaact tagtatctgc tggttttatt tattccaaca tgctctgagc aagctggaca
     3001 tgatttaccg tactctttgc tgattcttca atgtagtcgt ctgctgcatc taaaatcttg
     3061 tctgcggcat tttccaacgc atcggccatc tctccatgag taaatgctgc aaatccgtgt
     3121 gcgccctgca cctctgcgag tactgagtcc acacgctcca gatcagtttg ggaaagttct
     3181 tctggcaaat cagcaaaatc ggtcataaga ttagtgatcg tttcttcatc caatccctga
     3241 tttctcattt cttcaaacag ctgtgttttt gttaattgca tatcttctcc ttatttagtt
     3301 ttgtattact taagctccaa gccttcttag ccaaccacgt aaacccctaa ccgatctttc
     3361 tcttttcgta gcatcttcct gcttttgcct ctgctcttga gctttttctt ctagcctgtt
     3421 tacgattggt gtaggcccat cttcggcaac ttctccagcg gcatcaatct tcataagctg
     3481 cttgtccact gcgtccatac cagctcttac tttgctaact gctcccctta gactatccac
     3541 tactacttct gctgctttag ctttgctagc agctttatac tcttctactt tcatccctgt
     3601 cgcatcaatt ttttcagcga ctccagttgt aactcgatcc gctgtatcta caagtttagc
     3661 tccaagtttt gccgcttctg ctctagctcc cgcctgtatg agatcaactt tcgcaactat
     3721 tggactatcc acaatcatct gaccagtttc tctaactgcg tccttagcct tgtttccagc
     3781 agcatctttt agttgaccta ccttttccca cgccttttta cctgcatctt tgaattttgc
     3841 aaacctttct ccccatctgc cttttctttg agtaatgcga tcttgtcttt cttgcgcccg
     3901 ttgttctgcc gcacccttaa tttttgacgc agcttctgca atattcctag cttttgctga
     3961 tccatattca ctcaaacccg taccaccatc attcccctgg cccgcatgtt caatataatt
     4021 tctcacaact ccaaaggcat tattaaactg atctggattg aacactcctc catgcttggc
     4081 tctaaccgag tttatggctt gatcaacact aattgactca ccattacgaa gaccaagaac
     4141 gttgtctaat atatcaactc tatatcttag atcaaagtca gcctcttcgg gagcttgata
     4201 agcatcatct tttaagcgtt ctgcaattct ttggttatac tccctcaatt tgttttcaag
     4261 aagccttaca agggctggat cttccggacg tttaagtgta actttagtaa gatcctctgg
     4321 acccgcgcct gcagcaactt cggcaccagc cgaattcgcc aattgagctt ctagacctac
     4381 tactggatta ccattttctg tcatatatct cctatgttgt agtattacta tttctgtctc
     4441 caattgtacg taaaaacatt ttgaggccat caaacgcatt tgatgacttc gttgtaacaa
     4501 ccgactggtc ttgaacgcct gtctgaattg cagtcttgtc tattagtgaa cttaattctt
     4561 ccaaaaatac tgactcattt tgtgatgggt tgggttttgt ggccaattca attagtatct
     4621 ggagatagtc ctccatctgt tggttggttt gggctgccgt cggcacaaca tcaaaccgct
     4681 tatttgcagt taaatcagtc aagtctctca ggttacttaa ttcttggtcg gcactaaaag
     4741 agtgacgggt gctttttttc gaatcaagca gtttcttcaa cacaaccaag gcagactgaa
     4801 aattgtcctc ctcaacagag acaggcttac tgactggttg ttgtgaaaca ccaaattgtg
     4861 aattagtagt attggttctg cgaacgactt cttcctcaat atattgctct atgtctagcg
     4921 ataattgatc taggatagtt tgtaatttca tacatccact ttacacacgc gaagatttgg
     4981 tgtcaacact cattgtctgc caaatttaga ttgggcacgc accggtttca cacttgagat
     5041 gttcccagtc gatgtcttca aaaagttctt catctttttc aatctcatcc aagacacgca
     5101 taaactcgct aatggtcact ggctgctcgg gctggtattc ataggcagtg gaatcctgct
     5161 gaggcatgac gctgcaacac ttaacctgag attggtattg cttgaccatt tcggtaaatt
     5221 cggcaaactt gaccttagtt ggatcgtact tgagggtgta gctaacttgg ttaccagtat
     5281 cttccaaagg gttaccttgc tcatctaccc caacgatcca atatttttct agcaacatca
     5341 accatttgta ttgctcttct ggtgtggcct ctgcagcggt gaccaactca ccgttcatgc
     5401 caatccgaca aatcagtggt tgggtaggaa aaccaaccgc ggtggaacct tcgtacgatc
     5461 gcagttcttt gatgggatac ccctgatctt tatatttttt cacttgcgga tcgtcactcc
     5521 ggtactgcac ccaacgaata tattcgcgca tgctgggcaa gtgtgcccct tcagtcagtg
     5581 cgaaaagttt ggaaatcgtg cccgatggct taatagtggt attggtgtga ggcaccgtta
     5641 cgcccagctt tttggagtac tgctctgcct catccactac cgcgcgcttg aagcgtgcga
     5701 ttgttaacca aaagtcttta ctttttgttt cgtcaatcaa atcgcgaaac gaataaccaa
     5761 acatattcca accaaattcg tgaatccctg tcataccgac accaatccgg ttggtacgct
     5821 tcacttcgcg gttatacaaa caatccatag tatttacccg aatgagcgcg cgcgtggcag
     5881 ctcgataagc ttcttcggca tcatccaccg ttggacaata atatggcact acatcggcaa
     5941 tcacacagta gccacccaac atgtttaaac taatctcgcc acagggatta gggatttgtt
     6001 gatagacttt tgcctcagca ttacgggcga ttctggccaa cattttttcg gtgcgtgtca
     6061 gcggcttata cttgaggcct tcggcaaact tgccatcttg atagcctgcg tatccctcat
     6121 cattttgcac taagcgatgt tggtttatga atcctggctc accggtacca tcatggtaac
     6181 tggcttctaa gataaggttg agcactttt
//
LOCUS       DAOQZP010000049         6187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_12998_length_6187_cov_5.821265, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000049 DAOQZP010000000
VERSION     DAOQZP010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12998_length_6187_cov_5.821265"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1306
                     /locus_tag="PKH60_03995"
     CDS             104..1306
                     /locus_tag="PKH60_03995"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNQ17234.1"
                     /translation="MPQLCLYLHLHQPLRIAPYEVFDISSGRTYFATAESDDNRSIFQ
                     KVADKSYRPMLSLLGKLLHEEPDFRFALSITGVFIEQAQWYAPDVLNLLQELVKSDRV
                     EIIAETYHHSLASLYSPKEFERQVRLHQEVIKHHFKYDSVTFRNTELIYSNHIAEQVK
                     QLGFIGTLTEAVDRYLGNRPKTRVYGSMGWPQLPLLLKHAQLSDDIAFRFSDKSWPMF
                     PLHSEHYFDWLQAYAQEEIVNLFMDFETFGEHQWQDTGIFEFIDALVRKVIADPYGKF
                     VVPREIFEALDPEIPLPVYDVPDPISWADVDRDLTAWVDNVYQKDTLRLVYELESPIL
                     TSNDSKLINDWRNLQTSDHFYYMCTKWSADGDVHAYFSPYESPYEAYRRYSTVLADLM
                     GRLGVATS"
     gene            1328..3307
                     /locus_tag="PKH60_04000"
     CDS             1328..3307
                     /locus_tag="PKH60_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNQ17235.1"
                     /translation="MPRALTLGNGKFLVCLDKYGFVRDMYYPYVGLENHVAGQKHRIG
                     FQINQVFSWLDDGSWQISIGYKPETMVGYLVCKNDKLQATVVMEDIVYNETDVFIRQV
                     DVYNHSDVEMDIKVFFHQVFHISESKKRNTAFYDPTHNSIVHYKGRRVFIINGRTTTG
                     GAIDDYTVGAYEYDNKEGSFRDAEDGVLGKNAVEHGSVDSVVRFSLNCLPKEKARVFY
                     WICAGRSLDQAYALNEIVMQKTPAAMLHSTEAFWHSWLKTNAADTSTLTKEQKKLFDT
                     SLFILRCHTDNRGGIIASADSAMIEYGKDDYSYMWPRDAAFIVSTLARAGYDEVTKPF
                     FIFCKEVLHPDGYLHHRYRPDKSLGSTWHSTTSQTDWLKDKILQLPIQEDETASVLFA
                     LWQHYTTSKDLEFIEMLYKPMIEKMAEFLVSFRDKETGLPLPSYDLWEEKIGISTYTC
                     SAVYAGLMAAAQFCELLDKRDRMRKYKQTAQEVKDAAIKHLFSEDLDAFVRIAYQDSQ
                     AGIRQEKVVDASSLFGLWYFGMLEQSHPLFQKTLAQVRIRLRDPAQVGGYIRYENDAY
                     FKDTDISNPWIITTLWEALRKLYKPEVTSHDLEYVEEVLDWVVKHRYNSGVLAEQLNP
                     YTGESRSATPLVWSHAVYIETILKYLEKRTQLTVT"
     gene            3304..4224
                     /locus_tag="PKH60_04005"
     CDS             3304..4224
                     /locus_tag="PKH60_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HNQ17236.1"
                     /translation="MTERFFLGIDGGGSKTSVVCVNEAGSVVGQGSAGPTNLTSTSVG
                     AASLNLREGVRQALENLPQDKTIVKLVMGLAGMDSESEHDVAIEVFAKIIHPFGIEQF
                     ELVNDSVIALENGSNNSNAFVLISGTGSNCHGRNAQGKTAKAGGMDYLLADQGSGYAI
                     GRQVLLAAVKSYDGRGKKTILEQLVCQHFGLSDCSSVKTAVYQPPLNKLEIGQFATLC
                     RQAVDQGDEVALAIFDEAVEELLAHIASVLRQLDLESTPTEGVLSGSVLDIPYIREKL
                     IWLSGERWPQVTLSTPTQPPVWGAIKLAMK"
     gene            complement(4229..4711)
                     /locus_tag="PKH60_04010"
     CDS             complement(4229..4711)
                     /locus_tag="PKH60_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17237.1"
                     /translation="MQPHSMCALGMAGYVWGGVEVPCPSSASMKVIYQDKYHYYKTNV
                     YYLSNIKEDDDHKRMSVDIVRGGGGIACTITITRRSAEEGAEIEMTHSQQHVSGLREV
                     SRISHTQVSEGRHYNSMKYGDFSGKFTQSDRKRMQLMCDIKRDDGSFMSVQYTLEITI
                     "
     gene            4792..5838
                     /locus_tag="PKH60_04015"
     CDS             4792..5838
                     /locus_tag="PKH60_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HNQ17238.1"
                     /translation="MRLRHNFPTQQEKRYRWFMTTINTALSFDSNDKAKLKLHVVELL
                     EKTNWQTVQIAFPGISRATAFRWRQHYLQSGKKLQSLIPRSTKPKNVRQMVVPPQILG
                     VLKQWRKQYPHLSKYKLKVFLDLWCVEQGLERRSVSWIGKVLSRHQFFFAVRHPVKRR
                     RRRPRSGYRIYKCPNVSKVSLGYLQLDGVKVCWNGETCYFLSALEVKTRQAWVVRCPR
                     INSSHTKRLLETVLGQVSYTIHTIHTDNGSEFKAYFDQAVQDLQLTHLWSPPRTPKVH
                     AHMERFNKTLQEEFVDYYIDEAFINPELFNQKLTQWLIYYNTKRPHHSLGLLTPHQYL
                     VHLQKGDAQSLKCP"
     gene            5900..>6187
                     /locus_tag="PKH60_04020"
     CDS             5900..>6187
                     /locus_tag="PKH60_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17239.1"
                     /translation="MQNSTMPTGEMGEERGGIVQNDLDLRVDRAVQGLDESDAAHSIT
                     SIEKLLEELREKQNAEQVGVPGESDGQTDQTDSPVQAQIEAALRDCQRWWQP"
BASE COUNT         1798 a         1238 c         1397 g         1754 t
ORIGIN      
        1 cttggatgag cgaaattacc tcctttttcc gttcaagtga caaaattgaa gggtttttca
       61 tacgattgag tataacagcc tcatttcgtg ctatagtttt tttgtgccac aattgtgttt
      121 atacctccat ttacaccaac cactgagaat agcgccatac gaggtttttg atatttcctc
      181 tggaaggacc tattttgcaa ctgcagaaag cgatgataac cgaagtattt ttcagaaagt
      241 cgccgataaa tcgtatcgcc caatgttgag tctgctcggc aagcttttac atgaggaacc
      301 agattttagg tttgctctct ctatcactgg tgtgtttatt gagcaagccc aatggtatgc
      361 accagatgta ctcaatttac ttcaagaatt agtcaaaagt gatcgagtgg aaattattgc
      421 cgaaacgtat caccactcac tagcgagttt atattctccc aaggagtttg aacgtcaagt
      481 taggttgcac caagaggtaa ttaaacacca ttttaagtat gattctgtca ccttccgaaa
      541 taccgaatta atttattcca accacattgc tgagcaagtc aagcaattgg gttttattgg
      601 tactttgact gaagctgtgg atcgctattt gggaaataga ccaaagacta gggtttacgg
      661 tagtatgggc tggccgcaac tccccctact cttgaagcat gctcaactgt ctgacgacat
      721 tgcttttcgc ttttcagata aatcttggcc gatgtttcct cttcattcag aacattactt
      781 tgattggttg caagcttatg ctcaagaaga gattgtcaat ttgtttatgg attttgaaac
      841 ttttggtgag caccaatggc aggacactgg tatttttgaa tttattgatg cgctggttcg
      901 caaagtcatt gcggatccct atggtaaatt tgtggtgcct cgagaaatat ttgaagcgct
      961 tgatccagaa attcctctgc ccgtttatga tgtgccagat ccaatttctt gggcagatgt
     1021 agatagagat ctaactgctt gggtggataa cgtctatcaa aaggacacac ttcgtctggt
     1081 gtatgagttg gaatcgccca ttttaacgtc aaatgattca aaattaatca atgattggcg
     1141 taatttgcaa acgagtgacc acttttatta tatgtgcaca aaatggtcag cagatggcga
     1201 tgtgcatgcc tatttttctc cctatgagtc gccttatgaa gcctatcgca ggtatagtac
     1261 agtgctagcc gatctgatgg gcagacttgg ggttgcgaca tcgtaattca gttgctagga
     1321 tggatcaatg cccagagctc ttacccttgg aaatggaaaa tttttagttt gtttggataa
     1381 atacggcttt gttcgtgata tgtactatcc ctatgtggga ttggaaaatc atgttgcagg
     1441 tcaaaaacac cgaatcgggt ttcaaattaa tcaagttttt tcttggcttg atgatggtag
     1501 ttggcagatt tcaattgggt ataagccaga aactatggtg ggctatttag tttgcaaaaa
     1561 cgataaactt caggcaaccg tagtcatgga ggacatcgta tataacgaaa cagacgtttt
     1621 tattcgtcag gtagatgtct acaatcattc agacgtcgaa atggatatta aggtgttttt
     1681 tcatcaggtt tttcatatta gtgaaagtaa aaagcgaaat actgcgtttt atgatccgac
     1741 tcacaattcg attgttcatt acaaaggtcg tcgtgtcttc attattaatg gaagaaccac
     1801 cacgggtggt gcaattgatg attataccgt tggagcctac gaatatgaca ataaagaggg
     1861 ttcttttcgc gatgcagaag acggcgtttt gggaaaaaac gcagtcgagc atggctcagt
     1921 agattctgtg gtgcgctttt cactcaattg tttaccaaaa gaaaaggcca gagtatttta
     1981 ttggatttgt gctgggagat cgcttgatca agcgtacgcg ctcaatgaga ttgtgatgca
     2041 gaaaacacct gctgcgatgc ttcatagtac agaggcattt tggcattctt ggcttaaaac
     2101 aaacgctgca gatacctcaa ctttaaccaa agaacaaaag aaattatttg acacctcact
     2161 ttttatcttg agatgtcata ctgacaatcg tggcggcatc atcgcaagcg cagatagcgc
     2221 catgattgag tacggcaaag acgactacag ctatatgtgg ccaagagatg ccgcctttat
     2281 tgtctcaaca cttgcccgag ccgggtatga tgaagtcact aagccatttt tcattttttg
     2341 taaagaagtg ctacatccag atggttacct ccaccatcgc tatcgaccag acaaaagttt
     2401 gggttctact tggcattcca ctacttcgca aaccgattgg ttaaaagata aaattttgca
     2461 gttaccaatc caagaagatg aaaccgccag tgtgttgttt gcgctttggc aacactacac
     2521 cacttcaaaa gatttggaat ttattgaaat gttatacaag ccaatgatcg agaaaatggc
     2581 ggaattcttg gtttcatttc gagacaaaga aactggtttg ccgctgccat cctatgatct
     2641 ttgggaagaa aaaattggta tttccacgta cacttgttct gcggtctatg cgggtttgat
     2701 ggcagcggct caattttgtg aattactcga taaacgcgac cgaatgagga agtataaaca
     2761 gacagctcag gaggttaaag atgccgcaat caagcatctg ttcagtgaag atcttgacgc
     2821 cttcgtacgc attgcctatc aagattcgca ggcaggcatt cggcaggaaa aagtggtgga
     2881 tgctagctcg ttatttggct tatggtattt tgggatgctt gaacaatcac atccattatt
     2941 tcaaaaaaca ttagcacaag tcagaattcg tcttcgtgat cccgctcaag ttggaggcta
     3001 tattcgctat gaaaatgatg cttattttaa ggatacggat atttctaatc catggattat
     3061 taccacactt tgggaagcgc tcagaaagtt atacaagcca gaggtgacgt cacacgattt
     3121 ggagtatgtt gaagaagtgt tggattgggt agtaaaacat cgctataact ccggggtgtt
     3181 ggccgagcaa ctcaatccat atacgggtga atctcgatca gctacgcctt tagtctggtc
     3241 gcacgcagtg tatattgaaa cgatcttgaa atatcttgaa aaacgtacac aattgaccgt
     3301 cacatgacag aacgtttttt tcttggcata gatgggggtg gcagcaaaac cagcgtagtt
     3361 tgtgttaatg aggctggtag cgtggttggg cagggtagtg caggaccaac aaatttaaca
     3421 tcaacaagcg tgggcgccgc ttcgctgaat ttgcgtgagg gtgtgaggca agcgttggaa
     3481 aatttgccac aggataaaac catagtaaag ttggtgatgg gtttggctgg gatggattct
     3541 gagtctgagc atgatgtggc aattgaagtt tttgctaaaa ttattcatcc ttttggtatt
     3601 gaacaatttg aattagtgaa tgacagcgta attgcgctgg agaatggaag caataactca
     3661 aatgcttttg ttttaatttc tggcactggc tctaattgcc atgggagaaa tgctcaaggc
     3721 aaaaccgcaa aagctggtgg tatggattat ttattagcag atcagggttc cggatatgcg
     3781 attggtcgcc aagttttgct agcggcagtg aaaagctatg atggaagggg aaaaaaaaca
     3841 attttggaac aactagtttg tcagcacttt ggtctttccg actgctcttc cgtgaaaacg
     3901 gcggtatatc aaccgccgct caataagttg gagattgggc aatttgcaac attatgccgg
     3961 caggcagttg atcagggaga tgaagtggcg ctcgcaattt ttgacgaagc agtggaggaa
     4021 ttgctcgcgc atattgcatc cgtgttacga caattagact tggaatccac tccaactgag
     4081 ggagtgctct ctggatcggt gctcgatata ccctatatca gagaaaagct gatttggttg
     4141 tctggtgaac gttggccaca agttacctta agcactccaa ctcaaccacc ggtttggggt
     4201 gcgattaagc tggcgatgaa gtaagtcgtt aaatcgtaat ttcaagtgta tactggacag
     4261 acataaaaga accatcatcg cgcttaatat cgcacatcaa ttgcattcgt tttctatcag
     4321 attgtgtaaa tttgcccgag aaatcaccat atttcatgct gttgtagtgt cttccctcag
     4381 acacttgagt gtggcttatt ctcgatactt ctctcaatcc ggatacatgc tgttgtgagt
     4441 gggtcatttc aatttctgca ccctcctctg ccgatcttct tgttattgta atagtacatg
     4501 cgatgcctcc gccaccccta acaatatcta cactcatacg tttatgatca tcgtcttcct
     4561 tgatattgga taagtaatac acatttgttt tatagtagtg atatttgtct tgataaataa
     4621 ctttcattga agcagaggaa ggacagggaa cttcgactcc accccaaaca tacccagcca
     4681 taccgagagc acacattgaa tgaggttgca tataggcgat attatacctc atcaactctc
     4741 catatgaata ggtcacgata catttgagac tttggcgagc gaaagcgagc catgagacta
     4801 agacacaact tccctaccca acaagaaaag cggtatcgtt ggtttatgac caccataaat
     4861 accgctttga gctttgattc taacgacaaa gccaaactaa aactacatgt agttgagtta
     4921 ctagagaaga caaactggca aacagtacag attgcctttc ccggtatcag tcgagcaact
     4981 gctttccgtt ggagacagca ctacttacaa tcaggcaaga aactccaaag tctgattcct
     5041 cgatcaacca aaccaaaaaa tgttcgacag atggtagtac cgccacagat cttaggtgtg
     5101 ctcaaacagt ggaggaagca gtatccccat ctctctaagt acaaacttaa agtatttctt
     5161 gatctgtggt gtgtggaaca gggactggag cgacgttcag tatcctggat cggcaaggtg
     5221 ttgtcacgac accaattctt ctttgcggta cgacacccag ttaaaaggag acgaaggaga
     5281 ccaaggagtg ggtatcgcat ctacaaatgt ccaaatgtat ccaaggtgtc gcttggatat
     5341 ctccaactgg atggtgtaaa ggtctgctgg aatggggaga cttgctattt tctatcagcc
     5401 ttagaggtaa agacaagaca agcatgggtg gttcgctgtc cacggattaa tagttcacat
     5461 accaagagac tgcttgaaac ggtacttggg caagtatcct ataccataca caccatccat
     5521 accgacaatg gttcagagtt caaggcttac tttgatcaag cagttcaaga cttacagttg
     5581 acccacctat ggagtccacc gagaactccc aaggtgcatg ctcatatgga acgatttaac
     5641 aaaaccctcc aggaagagtt tgtggactac tacattgatg aggcattcat caacccagag
     5701 ctattcaatc aaaaacttac tcagtggctt atctactaca acaccaaaag accccatcac
     5761 tcacttggac tcctcacacc ccatcagtat ttggtacact tacagaaagg agacgctcaa
     5821 agtctcaaat gtccgtgacc agcacacaac cattgtaaca aactgacaca tagtttagac
     5881 ttcaattagg agaataacta tgcaaaattc aacaatgcct acgggcgaaa tgggagaaga
     5941 gagaggtggg atagtacaaa atgatcttga tttacgagtt gatagagcag tgcaaggctt
     6001 ggatgagagt gatgcagctc actcaattac tagtattgaa aaattgttag aagaattgcg
     6061 cgaaaaacaa aacgcagagc aagtaggtgt acctggtgaa tcagatgggc aaacagacca
     6121 aactgattcg ccagtacaag cgcaaatcga agcagccctt cgtgattgtc agcgatggtg
     6181 gcagcca
//
LOCUS       DAOQZP010000050         6173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_13030_length_6173_cov_5.202354, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000050 DAOQZP010000000
VERSION     DAOQZP010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13030_length_6173_cov_5.202354"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PKH60_04025"
     CDS             <1..437
                     /locus_tag="PKH60_04025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019239008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ17240.1"
                     /translation="RAREVKKQELDPSTLVLSETKYLTSIGTELFLQYKGQTIGLHSE
                     EKLAGFLRLSLPIETPPIDELKSCALIREVHVYGQAVPVGDKKNEHSQHLGLGKKMID
                     RAKELAKIAGYEKLAVISAIGTREYYKKLGFDLGDLYQFIQL"
     gene            complement(424..750)
                     /locus_tag="PKH60_04030"
     CDS             complement(424..750)
                     /locus_tag="PKH60_04030"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HNQ17241.1"
                     /translation="MTLQQLQAEYLLIKAIIDEKWPIAEKNQRVFARTMKLVEELGEL
                     ADELLSSMNLQRQDKQDAFHDQKVEDEFADVLASVVLLAAELDIDIEEVMKRKIEYTK
                     KRLTAE"
     gene            complement(771..956)
                     /locus_tag="PKH60_04035"
     CDS             complement(771..956)
                     /locus_tag="PKH60_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17242.1"
                     /translation="MGFLPNEVRPQRQIDFATYIYGPKHNGFTPTGKNIVKPTSPSES
                     IAANSVEEADGNSTEKQ"
     gene            complement(1010..2470)
                     /locus_tag="PKH60_04040"
     CDS             complement(1010..2470)
                     /locus_tag="PKH60_04040"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HNQ17243.1"
                     /translation="MRPTEILQHYWGYDRFRPGQQAIVEAILEGKDTLAIMPTGGGKS
                     ICFQVPALLLPGLTLVISPLISLMKDQVDALSRRSIPAEYLSSSQTPEEQRSVYGKLN
                     SNQLKLLYLSPERLQSRRFLTLIKSLNISLVVIDEAHCISEWGHDFRPEFRMIAPFVA
                     TQAVRPTVAAFTATATPKTQTDICQSLQLHSPFKRVSSFARNNLGLQVISCPSHFIQQ
                     MLVFKLLLKHRNESGIIYVSSRQTAENLTKIVRSLFPFSSIEAYHAGLAAQQRTDIQE
                     RFITDQIKMVVATNAFGMGIDKPNIRFVIHFHIPGSLEHYYQEVGRAGRDGKSSQTYL
                     LYNENNLSIHLGLLDQNEQITDKHRKQQLFKLHAMRTFSITKGCRMQLALRYFGEITK
                     KVCGQCDRCLAQICQSKALENEVSFEIDLLNQLEKLRGSLAQSFRLTPSNIATDKVLY
                     QLALIRPTTAEHAGKLAGIGLGWIKQWWDKFTPLLS"
     gene            complement(2513..3697)
                     /locus_tag="PKH60_04045"
     CDS             complement(2513..3697)
                     /locus_tag="PKH60_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNQ17244.1"
                     /translation="MQPLADRLRPNTLDEFVGQAHLVGKMKPLKVAHDMGHIFSMIFW
                     GPPGVGKTTLARILARAANKEFYELSAVSAGKADIKKIIDDHKGQRGLEQSYNPPLLF
                     LDEIHRFSKAQQDYLLPFVEKGDLVLIGATTENPSFEVIAPLLSRSKVFVLSQLTEIE
                     LSKIIDSALIELKKTHGKVKLAKATREWLVTYAAGDARRLLTLIEHTNDLYKNLNLES
                     LQTTLQTTSLKYDKKGEEHYDTISAFIKSMRASNADAALYYLARMVDAGEDPKFIARR
                     MIIFASEDIGLAAPTALVVANAVFDAVHKTGYPEAQIMLAHGVAYLCRCKKDRSAYDA
                     FFAALADVKKFGNLPIPLELRNAPTKLMKELQYGKGYQMYPDKNHSLLPSKLKNRKYF
                     QQ"
     gene            complement(3747..4136)
                     /locus_tag="PKH60_04050"
     CDS             complement(3747..4136)
                     /locus_tag="PKH60_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17245.1"
                     /translation="MPKQERLRGIGRDKTEKLFFQAIQELNSFSINTSPSIRSPFPVA
                     DKLGVIRKTVTQPDFSVTRLETGQTMFVEITASSGNLPAKEAQLRVVQAAGVSNYIQL
                     SGDQVHMLSELAPEARLKALFQLFGWK"
     gene            complement(4149..5114)
                     /locus_tag="PKH60_04055"
     CDS             complement(4149..5114)
                     /locus_tag="PKH60_04055"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF001985.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNQ17246.1"
                     /translation="MKLKKYHIIGLGGTFDHFHAGHEKFLMFASDLSDILLIGVTNQS
                     IAKEKTYSNSIEPYAERMHAVENFCRAHKIPHEIIELSDSVGSTTSDNRIEALAVTTE
                     TVVGGKKINQLRKKLGMTELPMHICALFLAEDNHPLHSERIRAGECDRKGFIYSDLFN
                     QTITISEKQRLLLAQPQGKIVNQPSFLTQENAQPNPIILVGDSTIERFTTNKWTFHIG
                     VFDRMIQRKLAQGITLYLKPDLVVQNPPGSITPELARSIQALLRSDLTKPKYLFIQGE
                     EDLAAIPAILLSPLGSVTYYGQPKIGLVEIVSSENFKAQLLTHLV"
     gene            complement(5125..5925)
                     /locus_tag="PKH60_04060"
     CDS             complement(5125..5925)
                     /locus_tag="PKH60_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNQ17247.1"
                     /translation="MRKQEIESFQRYITIWYQKYGRHTLPWRLTTDPYAILVSELMLQ
                     QTQVSRVIPKYKVFLKRFPNLKSLKSSSLADVLIFWQGLGYNRRAKYLWQLAKTTQIL
                     PSSQVELEKLPGIGPYTASAICAFAFNQPTPMIETNIRSVFLYHFFPDQKNVSDRDLV
                     PLVSSAVDQSNPRRWYWALMDYGSYLKTILPNPNRRSKQHAIQSMFEGSSRQVRGEII
                     KILTQQKVIDRTLLFKTLKSNQLFFEEALEQLLTEGFIIEIDKQLSLK"
     gene            5950..>6173
                     /locus_tag="PKH60_04065"
     CDS             5950..>6173
                     /locus_tag="PKH60_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17248.1"
                     /translation="MKNFRLVIAGIIICVVGSMALVKLLSFFISAPTTPTESSGISLE
                     ASLPTPTPTPQCATLLFGGDMMFDRHIREKA"
BASE COUNT         1748 a         1220 c         1315 g         1890 t
ORIGIN      
        1 ttcgtgccag agaagtcaag aaacaagaac ttgacccaag cacgctagtt ttgagcgaaa
       61 cgaaatatct gacatccatt ggtacagaac tatttttaca atataaaggt cagacgattg
      121 gattgcattc agaggaaaaa ctagcaggat ttttacgatt aagtcttccc atagaaacac
      181 caccaattga tgaattgaag agctgcgctc tcattcgaga ggttcatgtg tacggtcaag
      241 ctgtgccagt tggagataaa aaaaatgaac actcacaaca tctgggttta ggcaaaaaga
      301 tgattgatag agccaaagag ctggcaaaaa tagcaggcta tgaaaaactt gcagtaatct
      361 cagcgatcgg tactagagag tattacaaaa aattaggatt cgatctggga gatttatatc
      421 aatttattca gctgtaaggc gtttttttgt gtattcgatt ttgcgcttca tgacttcttc
      481 gatatcaata tctaattctg cagcgagtaa tactactgag gcaagcacgt cggcaaattc
      541 gtcttctact ttttgatcat gaaacgcatc ttgcttgtct tggcgctgga gattcatgct
      601 cgagagcaac tcgtcagcca actcgccaag ttcctccacc aacttcatcg tacgtgcaaa
      661 cactcgttgg tttttttccg cgattggcca cttctcatca ataatggcct tgattaacaa
      721 atattctgcc tggagttgtt gaagggtcat agaaatactc agtacttata ttattgtttc
      781 tcagttgaat tgccatcagc ctcttctaca ctgtttgcag caatagactc acttggcgat
      841 gttggtttta cgatgttttt tccggttggt gtgaaaccgt tgtgtttagg accataaata
      901 taagtggcaa aatcgatttg acgttgaggc ctaacttcgt tcggtagaaa acccatatgt
      961 tctcctgtct aatatactat aggacagtat atcagtttct tatatttcgt caagagagca
     1021 atggggtaaa tttgtcccac cattgtttta tccatcccag ccctatccca gctaatttgc
     1081 cggcatgctc agccgttgtt ggccgaatta atgctaactg atacagcact ttatctgtgg
     1141 caatgttact aggagtcaat ctgaacgact gagctaaaga accacggagt ttttcaagtt
     1201 gattaagtaa atcgatttca aaagacactt cattttccaa ggccttagac tgacaaattt
     1261 gtgcaagaca gcgatcgcat tgaccacaga cttttttcgt aatttcacca aagtaacgga
     1321 gtgccaattg cattcgacaa cctttagtga tcgaaaacgt tcgcatggca tgaagtttaa
     1381 acaactgttg ttttcgatgt ttatcagtaa tttgctcatt ctgatcaagt aagcctaaat
     1441 gaatactgag attattttcg ttatacagta agtaggtttg gcttgatttg ccatctcttc
     1501 ccgctctccc cacttcttgg taataatgct ccaaactgcc aggtatgtga aaatggatca
     1561 cgaatctgat gttgggttta tcaattccca tgccaaaagc gttggtcgca actaccatct
     1621 tgatttgatc ggtaataaac ctttcttgaa tgtcggttcg ttgttgagcg gcaagaccag
     1681 cgtgataggc ctcaatacta ctgaatggaa aaagacttcg cactattttc gtcaggtttt
     1741 ccgcggtttg gcgactagaa acatatataa tgcccgactc gtttcgatgt ttgagtagta
     1801 acttgaatac gagcatttgt tgaataaaat gactcggaca ggaaatcact tgcaaaccaa
     1861 gattatttcg agcaaaactg cttactcgtt tgaagggtga gtgcaactga agcgattggc
     1921 aaatatcggt ttgagttttt ggtgtggcgg ttgcagtaaa cgcggctaca gttggtctaa
     1981 ctgcttgcgt agcaacgaaa ggagctatca tgcgaaattc tggtcgaaag tcatgtcccc
     2041 actcagaaat gcagtgagct tcatcgatga ctaccaatga gatgtttaaa gatttgatta
     2101 acgtgagaaa tcttctagat tgcagtcttt ctggcgatag atacaggagc ttgagttggt
     2161 tagagtttag ttttccgtag acagaccgtt gctcctctgg agtctgtgag cttgataggt
     2221 actctgcggg aatggacctt cgactaagtg catcaacttg gtctttcatg agggaaatca
     2281 gtggagatat taccagagtt aagccaggca aaagcagagc tggaacttga aagcaaattg
     2341 actttccgcc tcctgttggc atgattgcca acgtgtcttt accttcaaga atggcctcaa
     2401 cgatggcttg ttgaccaggt cggaatcgat cataacccca ataatgctga agtatttcag
     2461 ttggtctcat gctgatgaaa ataccaacag aaactttcaa aaagcgtaga gattattgtt
     2521 gaaaatattt tctatttttt agttttgagg gtagaagcga atgattttta tctgggtaca
     2581 tttggtagcc tttgccatat tgcaactctt tcattaattt tgttggagca ttcctgagtt
     2641 caagcggaat tggcagattg ccaaattttt taacatctgc taaagctgca aaaaatgcat
     2701 cgtaagccga acggtctttt ttacaacgac agagatatgc cacaccatgt gccagcatga
     2761 tttgagcttc tgggtagcct gttttatgca cagcatcaaa aactgcattg gcgacgacta
     2821 gagcagtggg agcagctaag ccaatatctt cactggcaaa gataatcata cggcgagcaa
     2881 tgaacttggg atcttctccg gcatcaacca ttcgggcaag ataatacaaa gctgcatcag
     2941 cattgctcgc tcgcattgat ttgatgaacg cactgattgt gtcatagtgt tcctcgcctt
     3001 ttttatcgta cttcagcgaa gtggtttgca aggttgtttg gagcgactcg agattgagat
     3061 ttttgtataa gtcgttagta tgttcgatga gagtgaggag gcggcgggcg tcgcccgccg
     3121 catacgtcac caaccactct cgtgtagctt tagctaattt gacttttcca tgagtttttt
     3181 tgagttcaat tagtgctgag tcaatgattt ttgacaattc gatttcggtt aattgactca
     3241 gcacaaacac tttacttctg gaaagaagtg gcgcaatgac ctcaaaactt gggttttcag
     3301 tggtggcacc aattaaaaca agatcgcctt tttctacaaa tggcagtagg taatcttgtt
     3361 gagctttact aaaccgatgg atttcgtcaa gaaacaataa tggtgggtta taactttgct
     3421 ccagtcccct ttggccttta tgatcatcaa tgattttttt gatatctgcc ttacccgcag
     3481 agacagctga taattcgtaa aattctttgt tggccgccct tgccagaatt cttgccaatg
     3541 tagttttgcc aacccccgga ggtccccaaa aaatcatcga aaaaatatgg cccatgtcat
     3601 gcgccacttt aagtggtttc atcttgccta ccaagtgcgc ttggccgacg aattcatcaa
     3661 gggtgtttgg tcgtaatcta tctgccagtg gttgcatctt ccaatcatac aacaaaaaaa
     3721 cccgaaacag atagtttcat atagttttat ttccatccaa ataactggaa taacgctttc
     3781 aaccgagcct caggagctaa ctcagacagc atatgaactt gatcgccact taattgaatg
     3841 taattggaaa ctcccgctgc ttgaacaacc ctcaattgag cctctttagc aggtaaatta
     3901 cctgagctag cagtaatttc cacgaacatt gtttgaccag tttcaagtct agttaccgaa
     3961 aaatcaggtt gagtaacggt tttcctaatg actcctaact tatctgcaac aggaaatgga
     4021 gaacgaatgg aaggtgatgt attaatagaa aacgaattaa gctcttgaat tgcttggaaa
     4081 aacaactttt cggttttatc tctgccaata ccacgcagac gttcttgttt gggcatacta
     4141 gaagtcaatc ataccaggtg cgtcaacaac tgagctttaa agttttctga agatacgatc
     4201 tctaccaacc caattttcgg ttgaccatag tatgtcacag aacccaatgg agataacaaa
     4261 atcgcaggaa tggctgcgag atcttcttcg ccttgaatga aaagatattt tggtttagta
     4321 aggtctgacc gcagcaacgc ttgaatagac cgagctaatt ctggagtgat ggagccgggg
     4381 ggattttgca caaccaagtc gggtttcaga taaagagtaa tgccttgagc aagttttctt
     4441 tggatcattc gatcaaaaac gccaatgtga aatgtccatt tgttggtagt aaatcgctca
     4501 attgttgaat cgccaacaag aatgattggg tttggttgag cgttttcttg agtgagaaaa
     4561 cttggttgat tgacaatctt accctgaggt tgagcaagta gtaatcgttg tttttctgaa
     4621 attgtgattg tttgattaaa aagatccgag taaataaagc cttttctatc acactcacct
     4681 gccctaattc tttctgagtg aagtgggtgg ttatcttccg ccagaaaaag cgcgcaaata
     4741 tgcattggta attcggtcat tcccagcttt tttctaagtt ggttaatttt tttacctcca
     4801 accacagttt ctgttgtcac ggcgagtgcc tcgattcgat tgtcggaagt ggttgaaccc
     4861 actgaatcag ataattctat aatttcatgg ggaattttgt gcgctctaca gaaattttca
     4921 acagcatgca ttcgttcggc atatggttca attgaattag aatatgtttt ttccttagca
     4981 atactttgat tagtaacgcc gatcagtaga atatctgaca aatcgctagc aaacatgaga
     5041 aatttttcat gaccagcatg aaaatggtca aacgtgccac caaggccaat aatgtgatat
     5101 ttttttaatt tcactagagg gagtttactt gagcgagagt tgtttgtcga tctctattat
     5161 aaaaccctca gttagcagct gttctagggc ttcttcaaaa aatagctggt tgctttttaa
     5221 agttttaaat aacaacgttc gatcaatcac cttttgttga gttaagattt taataatctc
     5281 tcctctgact tgtcggcttg atccctcaaa catagattga attgcatgct gtttacttct
     5341 tcgattagga ttgggtagta ttgttttcaa gtaacttcca taatccatga gagcccaata
     5401 ccatcggcga gggttgcttt gatcgacggc tgaagaaacc aggggaacca aatcacgatc
     5461 ggacacattt ttttgatcag gaaaaaagtg atacaaaaaa actgaacgaa tattcgtttc
     5521 aatcattggt gtgggttgat taaacgcaaa agcacaaatt gcgctggcag tatatggtcc
     5581 aatgccgggc aacttttcta attctacttg gctagaggga agaatctgag tggttttggc
     5641 aagttgccat aggtatttag ctctgcgatt gtagccaagg ccttgccaaa aaatgagcac
     5701 atcagctaaa gagcttgact tcagtgattt aagattgggg aaacgtttga ggaatacttt
     5761 gtatttggga atgacgcgac tgacctgagt ttgttgtaac atcaattccg acaccagaat
     5821 tgcgtaggga tcagtggtca gtcgccaagg caacgtgtgt cgtccatact tttgatacca
     5881 tatagtaata tatctctgaa aagattcgat ttcctgtttt cgcatcacgg ctactataat
     5941 agcctaagaa tgaagaattt tcgtttggtg atcgctggaa ttattatctg cgttgttgga
     6001 agtatggcgt tagtcaaatt actgtcgttc tttatttcag cgccgacaac acccactgaa
     6061 tcgtctggaa tttccttgga agcaagcctt cccacaccta caccaactcc tcaatgtgcc
     6121 acattacttt ttggaggcga tatgatgttt gatcgtcaca tcagagaaaa agc
//
LOCUS       DAOQZP010000051         5982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_13548_length_5982_cov_4.186772, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000051 DAOQZP010000000
VERSION     DAOQZP010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13548_length_5982_cov_4.186772"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PKH60_04070"
     CDS             complement(<1..207)
                     /locus_tag="PKH60_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNQ17249.1"
                     /translation="MKETSARIILYAKNSGEVLMSKGDTGSVKFLGGKINTIDKSPLG
                     TAIREAREEGGIDLSKYESSIVELP"
     gene            complement(218..1258)
                     /locus_tag="PKH60_04075"
     CDS             complement(218..1258)
                     /locus_tag="PKH60_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNQ17250.1"
                     /translation="MQLSKTEKQIELELIRAIAVACVVYIHTASLIVNNLPKVSEFSW
                     FSLAFIDALVRWCVPVFIMVSGSLLLQKSEPHLQFVKKRLSRIGLPLLIWLPLYWLTV
                     ALFNPPLPNLRVLLQSVVYEQPYLHFYFLFVMLQLAILTPWLRSVVRSLSHRSLALMI
                     ILLIYIGMAYQSKTTNLFYLFIPYLGYYLYGYFARNFTFKRRLKLIAVSMIVSVSSLM
                     VIAQYFMQRGDLVIRGVANSTTVVSYLSPLVVVLSLLAFPVLKDPTVYRWFAKLINQR
                     AIVSIGECSFGIYLIHPILGQVLVYFLPKLHDVQLAHAIPVTLVLTMCLFFVSWAIAW
                     AIRRLKVVSWLV"
     gene            complement(1298..2425)
                     /locus_tag="PKH60_04080"
     CDS             complement(1298..2425)
                     /locus_tag="PKH60_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17251.1"
                     /translation="MFNSEGKKLVVSVSTLLVVALCLSILFDLVYRTVTHNGYNFPSH
                     LLINKEITNKYQIVKVGNSHAEDSLTFEKYNARSLDLAGVAQRFSYDMVLLKQHRKEI
                     EDNALIIISVSPISFSHKIADASDGLQYNYYGTVSPFLIPNLKVGDYIQQEFVPFLRS
                     GYLFRKWYTDKVEERISEEERWKEPEQQQLEVVVPPQAPTKGPILNIDPEEIPYNVEA
                     ILAELNSPSPISKERYIDNMNFIFNKWYHTEEFDPKYFEINRRDLEKLIAYCKKNNWR
                     PVLITIPISAVLQEGLLDDYMEKYLYENLDKTDLQGAEYIDFSNQLNVSTNNYLYSNA
                     DHLNKRGGAIFSYLLMNTLIQRGYLPPEIDNYDYRPLYRVE"
     gene            complement(2412..3848)
                     /locus_tag="PKH60_04085"
     CDS             complement(2412..3848)
                     /locus_tag="PKH60_04085"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNQ17252.1"
                     /translation="MIFSSFTFAIFFIIVFILYWQLPKKFQSTLLLFASYYFYFSWKP
                     VYGLLIIVTTAVTYYAALFIENYPSKKNRIFYLSLIIILGQLFIFKYYNLFSISTSSL
                     LNGLHIPFRFPQFQLLLPLGISFFTFQTLGYLIDVYRGNARAEKNLGMLALFVSFFPT
                     ISSGPIERGWHLITQFQKKQVFTYSQTVSGLKLFTWGLFKKLVIADNLGIVVSRVFDN
                     LPDYKGLSLLIAMLFFSIQIFADFSGYTDMARGVARMLGFSLFENFKTPYLATSIRDF
                     WRRWHISLSSWFRDYVYISLGGSRKGEIRTYINTILVFLLCGLWHGASWTFIIWGFAH
                     GLMISAERMLGKLFSKKITVPNSISILYAFSVITALWVLFRAKNFADALYIFRYSFTG
                     IKYFVSPHYWWATLSHLFRTNVVEMFIALFCVIVIVVTEIITNQESLSKIIIRQKKII
                     RFTIYVALVVGIILLRNSQAFEFIYVQF"
     gene            complement(3917..4963)
                     /locus_tag="PKH60_04090"
     CDS             complement(3917..4963)
                     /locus_tag="PKH60_04090"
                     /inference="COORDINATES: protein motif:HMM:NF020135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HNQ17253.1"
                     /translation="MHKKEENQISKEKLNPFKNVLIIGTGAIGSLFKVELTKNNEQLD
                     VNLVSHDTITEELLAGVEVIILATPNQTVKEMVVKLLEKIPEDSAVPTIVLIQNGVGV
                     VEQAHLALAESTNPDRRLSIVRASVFTQVTKKGDQLDYNAEKLRVALSPIGCSPEDFR
                     GVEQLLLSAHFQTRNYENYQSLEWTKLLFNTLGTTSMITGLPPSITFADKELYQLEMN
                     AFAQRCNILRQCGIPIAQIDWVKTQMLPVLENFRHISFFKPARKALAGLISSQRNNQP
                     SAAWQKVEAGGDLSEAIQYHQAFIDLAEQNNIEVPALDRAIVLLLLEQVNGTLNLQSI
                     SLEERKERLMLKLE"
     gene            5013..5654
                     /locus_tag="PKH60_04095"
     CDS             5013..5654
                     /locus_tag="PKH60_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17254.1"
                     /translation="MNKLLFVLALGILLSGCSLLPQKSSQVETPPLEQQVVKEYQKVA
                     DAMASGKSVECVMTNTQEGTSFTYQVKGNMVRSFGQLSPDSTSSGNMISDGTYLYMWS
                     DDSAQGTKMKIPSETEVAEMTEQPDDVLPDLSNEEKQQELEDLGYSIKCDEKDLANDV
                     FVPPTTIQFQDLSQMLESATQLQTEMESKASESTPELTQEQIEAMMKSFGDGE"
     gene            5673..>5982
                     /locus_tag="PKH60_04100"
     CDS             5673..>5982
                     /locus_tag="PKH60_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17255.1"
                     /translation="MLKVTAPKVLRQKNSKFFVVVRLLLVVSLIAIIVGLLLKNKSAS
                     DFSSTQGILLASTQAELQTIEDRLASASATIEKLLAEDQRVRNNQLEATISAIQKTYT
                     E"
BASE COUNT         2037 a         1161 c         1031 g         1753 t
ORIGIN      
        1 aggcaattca actatagaag actcatactt agagagatcg atgccacctt cttctctagc
       61 ttcacgaatc gctgttccta gtggagattt atcaatcgta tttatttttc cacccagaaa
      121 tttcacacta cctgtatcac ccttgctcat caaaacttca cctgagtttt ttgcatataa
      181 aattattctt gcgctcgttt ctttcataga tgcttactca aactaaccaa ctaacaactt
      241 ttaatcgtct aattgcccaa gcaattgccc atgaaacgaa aaacaagcac atagttaata
      301 ctagagtcac gggaatagcg tgtgctaact gcacatcatg aagtttgggt aaaaaatata
      361 cgagcacctg acccaagatt ggatgaatca aatagatacc aaaactacac tctccaattg
      421 aaacaatcgc tctttggttg atcagttttg caaaccaacg gtacacagtg gggtctttaa
      481 gcactggaaa agccaaaagg ctcagtacta caaccagtgg agaaagatat gaaacaacgg
      541 tggtggaatt ggctacaccc ctaataacta aatcacctcg ttgcataaaa tactgggcga
      601 taaccattag actagataca gacacaatca tacttactgc aattagcttc aatcggcgct
      661 taaatgtaaa atttctcgcg aaataaccat acaaatagta acctaagtag ggaataaata
      721 aataaaaaag attcgttgtt ttagattgat atgccatgcc aatatagata agcaaaatga
      781 tcatgagagc gagcgagcga tgggagagcg agcgcaccac cgaacgcaac catggcgtta
      841 aaatggcaag ctgcaacatc acaaataaaa aataaaaatg aaggtatggt tgctcatata
      901 caactgactg tagtaacact cttagatttg gaagcggagg attaaatagc gctactgtca
      961 gccaatagag tggcaaccaa atcaataatg gcaaaccaat tctactcaac cgttttttga
     1021 caaactgcaa gtgaggttcg cttttttgca ataacaacga accgctgacc ataataaaca
     1081 ccggcacgca ccaacgcacc aatgcatcaa taaaagcaag tgaaaaccaa gaaaattcgc
     1141 taacttttgg taagttattg actatgaggc tggcagtatg aatgtacact acgcaagcaa
     1201 ctgcaatggc gcgaatcaac tccaattcaa tttgtttttc tgtttttgat aattgcattt
     1261 tgacgacagg atcataccat ttccgatcaa aaatgcatta ctcaactcga taaagcggtc
     1321 gataatcata attatcgatt tctggaggta aatacccacg ttgtatcaat gtgttcatca
     1381 aaagataact aaatatcgct ccaccccttt tgttgaggtg atcggcatta ctatataaat
     1441 aattatttgt tgacacattt aattgattag aaaaatcaat atattccgct ccttgtaagt
     1501 cggttttgtc cagattttcg taaagatact tctccatgta atcgtctaat aaaccctctt
     1561 gcaaaacagc ggaaattggt atcgttatca acacagggcg ccaattattc tttttacagt
     1621 aggctataag tttttccaaa tcccttcgat taatttcaaa atattttgga tcaaattctt
     1681 ctgtgtgata ccacttattg aaaataaagt tcatattgtc tatatagcgt tccttactaa
     1741 taggagaagg agagttcaac tcagcgagaa ttgcttcaac gttatatgga atttcttctg
     1801 gatcaatatt taatattggt ccttttgtgg gtgcttgggg cggtacaaca acctcaagtt
     1861 gttgttgctc gggttctttc catcgttcct cttccgaaat ccgttcttca actttgtctg
     1921 tataccattt acgaaataaa tagccagatc ttaaaaaagg tacaaattcc tgctgtatgt
     1981 aatctccaac tttcaaattt ggaattaaaa atggactaac agtcccataa taattatatt
     2041 gcaaaccatc actcgcatca gcaattttat gagaaaatga aatcggtgag accgaaataa
     2101 tgatcaacgc attatcctca atttcctttc ggtgttgttt gagtaaaacc atatcatagc
     2161 taaatctttg tgctacacct gctaagtcaa gactgcgagc attatatttt tcaaacgtaa
     2221 gactgtcttc cgcatgagaa ttaccaactt ttacaatttg atatttattt gtaatttctt
     2281 tgttaattag caaatgagat gggaaattat atccattgtg agtaaccgtt cgatagacca
     2341 gatcgaaaag aatactcaaa catagcgcta cgaccagcaa tgtagaaact gatactacta
     2401 gttttttacc ttcagaattg aacatagata aactcaaatg cctgtgaatt tcttaacaag
     2461 ataataccga ctacgagtgc tacatagata gtaaaacgaa taattttctt ttgtctaata
     2521 attattttag atagtgattc ttgattagta attatttcag ttaccactat tacaattaca
     2581 caaaaaagtg caatgaacat ttccaccaca ttagttctaa ataaatgact gagcgtcgcc
     2641 caccaatagt gtggtgatac aaaatatttt atgccagtaa atgagtaacg aaatatatat
     2701 aaagcatctg caaaattttt agctctaaaa agaacccaga gtgccgttat aacagaaaat
     2761 gcatagagaa tactaatgct gtttggaact gttatttttt tactaaatag ttttccgagc
     2821 attcgctctg cagaaatcat taagccatgt gcaaaacccc aaataataaa agtccaggaa
     2881 gctccatgcc ataaaccaca aagtaaaaaa acaagaattg tattgatata agttctaatt
     2941 tcacccttcc gactgccacc gagcgaaatg tacacataat ccctaaacca ggaagagagc
     3001 gaaatatgcc atcgtcgcca aaaatcacga attgatgtag ctaaatatgg agttttaaag
     3061 ttctcaaaaa gcgaaaatcc caacattctt gcaacacccc ttgccatatc tgtataacca
     3121 gaaaaatcag caaaaatttg aattgagaaa aagagcattg caatcaaaag actcaatcct
     3181 ttgtaatcag gaagattatc aaacactcta cttactacaa tcccaagatt gtcagctatt
     3241 accagttttt taaacaaacc ccaggtaaaa agttttaagc cactcactgt ttgagagtaa
     3301 gtaaatactt gttttttttg aaactgagta attaaatgcc aacctctttc aattggacca
     3361 gacgaaatgg taggaaaaaa agacacaaat agtgctaaca ttcccagatt tttttctgct
     3421 cttgcgttgc ctcgatacac atctatcaga taaccaagtg tttgaaaggt aaaaaacgaa
     3481 atacccaaag gaagcaataa ctggaattga ggaaaacgaa aaggaatatg cagaccattt
     3541 aatagggatg aggtagaaat gctaaacaga ttgtagtatt taaatataaa taattgaccc
     3601 aaaataataa ttagtgataa ataaaatatt ctattttttt tacttggata gttctctata
     3661 aataacgcag cataatatgt aactgcagtc gtaacaataa ttagtaaccc gtagaccggc
     3721 ttccatgaaa aataaaaata ataacttgca aatagcagta aagtcgattg gaattttttt
     3781 ggcaactgcc aataaagtat aaaaacaata atgaaaaata tcgcaaaagt aaatgaagaa
     3841 aatatcatgc tctaatcatt atagtgtcaa ttgtgctatc tgcaaaccca ggtgttacgg
     3901 ctggtgtaga ttatcttcac tctaatttta acattaaacg ttcttttcgc tcttcgagtg
     3961 aaatggattg taaattaagt gtcccattca cctgttctag tagtaataac acaattgcac
     4021 gatctaacgc tggcacctca atattatttt gctctgccaa gtcaataaat gcttgatgat
     4081 actgaatcgc ttcggacaaa tcaccgccag cttcaacttt ctgccaagca gctgatggtt
     4141 gattattccg ttgactgcta atcaagccag ctaaagcttt ccgggctggt ttaaaaaatg
     4201 aaatatgtct gaaattttct agcactggta gcatttgtgt tttaacccaa tcgatttgtg
     4261 caatgggaat accgcattga cgcagtatgt tgcatctttg agcaaaagcg ttcatttcaa
     4321 gctgatagag ctctttatct gcaaaagtaa ttgagggggg taaaccagta atcattgaag
     4381 tagtacccaa tgtattaaat aaaagtttgg tccactcaag agattgatag ttttcgtagt
     4441 tgcgagtttg gaaatgtgca gataataaaa gttgctcaac tcctctaaaa tcctctggag
     4501 aacaacctat gggtgagaga gcgacacgta atttttctgc attatagtca agttggtctc
     4561 cctttttggt tacctgcgta aaaacacttg cccgtactat tgaaagtcgt ctatctggat
     4621 ttgtagattc tgcaagtgct aaatgtgctt gctccacaac accaacgcca ttttgaatta
     4681 aaactatagt tggtactgct gaatcctcag gaattttttc taacaacttg acaaccatct
     4741 ctttaaccgt ttgatttggt gtagccaaaa taattacctc tactccagcc agcaattctt
     4801 cagtaatagt atcatgactg acaagattta catcaagctg ctcattattt ttagttaatt
     4861 caaccttaaa cagacttcca attgcacctg ttccaataat taaaacattc ttgaacggat
     4921 taagtttttc tttcgaaatt tgattttctt cttttttgtg catcattaga tatcatacta
     4981 taactttaga acaatgatat actgaacacc atatgaacaa actactcttt gtcttagccc
     5041 ttggaatcct actgagtggc tgctcgctat tgccacaaaa atcctcgcaa gttgaaacgc
     5101 cgcccctcga acaacaagtc gttaaagaat atcaaaaagt tgccgatgcg atggcatcgg
     5161 gcaaatcggt cgagtgcgtc atgacaaata ctcaagaagg aaccagcttt acttaccaag
     5221 tgaaaggtaa catggttcga tcatttggac aattaagtcc agattcgact agttctggaa
     5281 atatgatttc tgatggcaca tatctctata tgtggtctga tgattctgca caaggtacaa
     5341 aaatgaaaat cccaagtgaa actgaggttg cagagatgac agagcaacct gacgacgtgt
     5401 tgccagattt aagcaatgag gaaaaacagc aagaattgga agacctgggt tactccatca
     5461 aatgtgatga aaaagacttg gcgaacgatg tgtttgttcc ccccacaacg attcaatttc
     5521 aagatttgag tcaaatgctg gagtctgcta ctcagttaca aacagaaatg gaatctaagg
     5581 ctagcgaatc gacaccagaa ctaactcaag agcaaattga ggcaatgatg aagtcgtttg
     5641 gtgatggaga ataaatcact tatactaatt gcgtgttaaa agttactgcc cccaaagtac
     5701 ttcggcaaaa aaattctaaa tttttcgtgg tggtcagatt gcttttggtt gtttctctga
     5761 ttgcaattat tgtgggtttg ctactaaaaa ataagtcggc atcagatttt tcaagcactc
     5821 aaggtatttt gcttgcatca actcaagctg aacttcagac aattgaagat cggctagcca
     5881 gcgcctctgc caccatcgaa aaactccttg ccgaagatca gcgggtcaga aataaccagc
     5941 tggaagccac catttccgca attcaaaaaa catataccga at
//
LOCUS       DAOQZP010000052         5563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_14883_length_5563_cov_3.894880, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000052 DAOQZP010000000
VERSION     DAOQZP010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14883_length_5563_cov_3.894880"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PKH60_04105"
     CDS             <1..284
                     /locus_tag="PKH60_04105"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNQ17256.1"
                     /translation="AQKQNNYIFTGDLNSPPDSPIVSNIEKNLVNAGPRYDQPTWTTK
                     PFDYHGWQEDKLCWRIDYAFATPDIKIQSAETLATTVSDHLPILISITL"
     gene            complement(274..867)
                     /locus_tag="PKH60_04110"
     CDS             complement(274..867)
                     /locus_tag="PKH60_04110"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNQ17257.1"
                     /translation="MSTRYLLRHCQYANPRNIIVGRLPVVLSEEGEREARKLRDYFAD
                     KQIQRIYSSAVERCKQTATIVADGKLELIHDQRILEGFSAYQGYWMDEQTPPWIDYYS
                     HRNELGGENVQDVQKRMVEFWQSLSTVTDNLILCSHGDPLYTLYSYLNHIDISDLEEA
                     VSQSRDYIPKGAFWQFEGEGEDWKLVGLTTQANLSTK"
     gene            complement(871..>1125)
                     /locus_tag="PKH60_04115"
     CDS             complement(871..>1125)
                     /locus_tag="PKH60_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17258.1"
                     /translation="TIIESYRNFLLGDKLRGVLEQIDGFVQEWSKYRESLEKFGRAVE
                     SLGTSYKELTTTRTNQLEKRVERVQNIGQKALSSQTKKES"
     assembly_gap    1126..1225
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1226..2138)
                     /locus_tag="PKH60_04120"
     CDS             complement(<1226..2138)
                     /locus_tag="PKH60_04120"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNQ17259.1"
                     /translation="MFENILLLALILIILIGFTIVVYLLSKRGGQTDAKTMRSLVDEV
                     FGLTNERVTAQSKQILESQREIIQTDLQNKQSQLNDLIKGMSELVDKRQVEIKLLEKE
                     RAEQFGSLAKQLQEQQRVTELLRSRTEELTRVLSSNQSRGGWGERIIEDLLRSNGLQK
                     GVHYLVQSDIGKTGQRPDITLLLPDHRMVPVDVKFPFSALQQWAGTDDVAQKKLLVKQ
                     FSQDVKKHIDKVATYILPQEGTLDYVVLFVPNEAVFSFLNQHLPEVVDYAMSKRVLIT
                     SPFTFLIVARTIIESYRNFLLGDKLRGV"
     gene            2226..2846
                     /locus_tag="PKH60_04125"
     CDS             2226..2846
                     /locus_tag="PKH60_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17260.1"
                     /translation="MVASQTVNRARSRLHQLPTSQRKTDWHQNAPIIAKKARSRLKKQ
                     FSKPHFSARQIKLFRSGLPRFFPSTTGFLELLNTFYLQRKPYLFFLLLGLLFICSIGL
                     VFTSVAPQNVANFPLYKSYGIVILLIFFTVSCLSIFFLAHFRRGVLVGLFSSVILLLH
                     FQHFTVQPIELIAVTTFFLVIELFLTLAEKLGGQIKPRPHHHRRRV"
     gene            2854..4308
                     /gene="metG"
                     /locus_tag="PKH60_04130"
     CDS             2854..4308
                     /gene="metG"
                     /locus_tag="PKH60_04130"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNQ17261.1"
                     /translation="MNNESFYITTTLPYVNAEPHIGFALEIVTADVIARFHRALGHQV
                     VFNTGTDEHGQKIYDRSQAENKDPQTFVDFHAQQFSLLKNQLNLSFDHFIRTTDPHHK
                     LAAQQFWQLCMENGYIYKNQYKIAYCVGCELEKQASELVDEHCPLHPNQQIEFREEEN
                     YFFKFSAFQQSLLDLYTAKPDFVKPEGKMREIESFVRNGLNDFSISRLKTKMPWGIEV
                     PGDPNHVMYVWFDALVNYISTLGWPEDRSVFNEFWPVTQIAGKDNLRQQAAMWQAMLL
                     AAKLPPSQQILINGFISIDGQKMSKSLGNVISPNDMVQRYGTDAARFLLLLLNPISQD
                     MDVTWERFDHLYTSTLSNGIGNLCSRVAKLASQISPPTNSPAQNQESALGFNVVYSNA
                     LQAYDFQKVTDWLLAEIQAADAFLSEQKPWLADESTKTTLVTQSVKMIQKIAYHLIIF
                     MPSIGEQLLNHFSQPTILPLTPLFPRLATPKNHV"
     gene            4301..5521
                     /locus_tag="PKH60_04135"
     CDS             4301..5521
                     /locus_tag="PKH60_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNQ17262.1"
                     /translation="MSNSIKRYFRRHPLRTQRGLEMLPGIFSWTLILFPIWGSFLVPN
                     LVAYYVITFAVYWVFRSFSLATLALSAHFTIKASSKYDWIKDLNQHYGSTWRKVHHLI
                     IIPTYQEPLETLERTLQSLSQQTIPLANIHIMLSFEEREGDAAKQKARYLTKKFSNTF
                     GNVWTTLHPDIKDEVKGKSANTNWGAQRANEFIVNNPSINSDLVTVTSEDADACFHPS
                     YFANVTFDFLSAEKPYNLIWQGAIVFYNNIWKVPAPIRVIAAVFSVTQMYILKRSDRL
                     INFSTYTTTLRHIREINFWDTDVIPEDYRLFFKSYFAKKGQFWARPIFLPIYADAAEA
                     KGMKQTFINQYEQLKRWAWGVSDDAYIIRQFVLSDEIPFWDKTIRVLKTIEDHFLWPV
                     NWFAITIAAFFPHC"
BASE COUNT         1589 a         1278 c          957 g         1639 t
ORIGIN      
        1 ttgctcaaaa acaaaataac tatatattta ctggcgatct gaactctcct ccggactcac
       61 cgattgtctc aaatatagaa aaaaacctgg ttaacgctgg tccgaggtat gatcaaccca
      121 cttggaccac caaaccgttt gattatcatg gctggcaaga agacaaactc tgttggcgaa
      181 ttgattacgc gtttgccact cccgatatca aaattcaatc tgctgaaacc ctcgcaacca
      241 cagtctcgga tcatttaccg atcttaattt caattacttt gtagataaat tagcttgggt
      301 ggttaatcca actaatttcc aatcttctcc ctccccttca aactgccaaa atgcaccttt
      361 gggtatgtaa tctctgcttt gtgaaaccgc ttcttccaaa tcagatatat caatgtgatt
      421 taggtaacta tacagcgtat agagaggatc accatgagaa cagagtatta agttatccgt
      481 aacagtactt aaactttgcc aaaattccac catcctcttt tgaacatctt ggacattctc
      541 gcctccaagt tcatttctat ggctatagta atcaatccat ggaggagttt gttcatccat
      601 ccagtagcct tgataggctg aaaaaccttc gagaattctt tgatcgtgaa tcaattccag
      661 tttgccatcc gcgacaatgg tggctgtttg tttacagcgc tcaactgcac tactataaat
      721 gcgctgaatt tgtttatcgg caaagtagtc tctcagtttg cgcgcctctc gctcgccttc
      781 ttcagaaagt acaactggca accgcccgac aataatgttg cgaggattgg cgtactggca
      841 gtggcgaaga aggtagcgtg ttgacatgtc tcaactttct tttttcgtct gactactcag
      901 cgctttctga cctatgtttt gcacgcgctc aacccgtttt tccaactggt ttgtacgcgt
      961 agtagtaagt tccttataac tggttcctag tgattctaca gctcggccaa acttttccag
     1021 tgattcacgg tacttactcc attcttgaac aaaaccatca atttgctcca acacgccgcg
     1081 caatttatca cccaataaaa agttgcgata actttcaata atcgtnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnacacg ccgcgcaatt tatcacccaa taaaaagttg
     1261 cgataacttt caataatcgt tcgagccacg attaagaaag taaacggaga agtaattaat
     1321 actcgcttac tcatcgcgta atctacaact tctggcaaat gttggttgag aaaagaaaac
     1381 actgcttcat ttggtacaaa taaaaccaca taatctaatg ttccctcttg aggaagaata
     1441 taagtcgcaa ctttatcaat atgttttttt acatcctgcg aaaattgttt gactagtaat
     1501 tttttttgag caacatcgtc tgtacctgcc cattgttgta gtgcagaaaa aggaaacttc
     1561 acgtccaccg ggaccatacg atggtcgggt aacaacagcg taatatctgg ccgctgaccg
     1621 gtcttgccga tatcgctttg aaccaaatag tgaacgcctt tttgcaagcc gttagaacgg
     1681 agcaagtctt caataatgcg ctcgccccag ccccctcggc tttgattgct agataagacc
     1741 cgagttaact cttcagttcg cgagcgcaat aattcggtca cccgttgttg ctcttgcagc
     1801 tgtttagcca aactgccaaa ttgttctgcc cgttcttttt ccaacagttt gatttctact
     1861 tgtcgcttat ccaccaactc actcatgccc ttaattaaat cgttcagttg agattgttta
     1921 ttctgaagat cagtctggat gatttcccgc tggctttcta atatttgttt gctttgcgct
     1981 gttactcgtt cgttcgttaa accaaatacc tcatccacca aactccgcat cgtttttgcg
     2041 tcggtttgac caccccgttt agataataaa taaactacta tggtaaaacc aataagaata
     2101 attagtatca gcgcaagtaa caaaatattt tcaaacatat agtttgattc tattgtaacg
     2161 agattatcaa ctgagagcaa accagacttg cgttagtctc tagaaactga ttagatacta
     2221 tcaatatggt agcctcccag acagttaaca gagcaagatc gagattgcac caattaccaa
     2281 catcacaacg aaaaactgac tggcatcaaa acgctcctat cattgcaaaa aaagctcgct
     2341 cgagactaaa aaaacagttc tccaagcctc atttttcggc aagacaaata aagctgttta
     2401 gaagtggttt accaagattt tttccttcaa caactggttt tttagaactt ctcaacacct
     2461 tctacttaca acgaaaacca tatctattct ttctattgct gggactactc ttcatttgca
     2521 gtattgggtt ggtatttacc agcgttgccc cacaaaacgt ggctaatttt cccctctata
     2581 aatcatatgg aatagtgatt ctgctgatat tttttactgt tagttgtcta tcgatatttt
     2641 ttcttgctca ttttagaaga ggtgtattag ttggtctatt tagtagcgtg attttgttgc
     2701 tccatttcca acacttcacc gtacaaccca tagaattaat tgcagttact actttttttc
     2761 ttgtaattga attatttctg accttagcag aaaaacttgg tggacaaata aaaccccgcc
     2821 cacaccatca ccgacgcagg gtataataaa tccgtgaaca acgaatcctt ttatatcact
     2881 actaccttgc cctatgttaa tgccgaaccg cacattggct ttgccttaga aattgttaca
     2941 gcagacgtca tcgcccgctt ccacagagct ttaggacatc aagtagtgtt taatacagga
     3001 actgatgaac atggccaaaa gatttatgat cgtagtcaag cagaaaataa agatccacaa
     3061 accttcgtag attttcatgc tcagcaattt tcgttactca aaaaccaact caacctttct
     3121 ttcgatcatt tcattcgcac aactgatccc catcacaaac ttgcagcgca acaattttgg
     3181 caactctgta tggaaaatgg ttacatttat aaaaatcagt acaaaatcgc gtattgcgtt
     3241 gggtgtgaac tagaaaaaca agcttctgag ttagtagatg aacactgccc cctacatccc
     3301 aatcaacaga ttgagtttag ggaagaagaa aattatttct tcaaattttc agcctttcaa
     3361 caatcccttt tggatttata cacagccaaa cctgattttg tgaaacctga gggaaagatg
     3421 cgcgaaatcg aatcatttgt aagaaatgga ttaaacgatt tttcgatttc gcgcctcaaa
     3481 accaaaatgc cttggggcat agaggttcct ggtgatccca atcacgtcat gtacgtctgg
     3541 tttgatgcat tggttaatta tatttcgact ctgggctggc cagaagatcg atctgttttt
     3601 aatgaattct ggccagtcac acaaattgcg ggcaaagaca atttgcgtca acaagctgct
     3661 atgtggcaag caatgctact cgcagctaaa ttgccacctt cccaacaaat tctaatcaac
     3721 ggattcataa gtattgatgg ccaaaaaatg agcaaatcgc tcggtaacgt catctctcca
     3781 aacgacatgg tacaacgcta tggcaccgat gccgctcgct ttctattact tttgctcaac
     3841 cccatttccc aagacatgga tgtaacctgg gagcggtttg accatctcta cacgtccaca
     3901 ctttccaatg gtattggtaa tctttgctct cgagtggcca aacttgcttc acaaatttct
     3961 cccccaacca attcacctgc tcaaaaccag gaatcagctc ttggatttaa tgtagtgtat
     4021 tccaatgccc tacaagcgta tgattttcaa aaagttacag actggctgct cgctgaaatt
     4081 caagctgccg atgcgttttt aagcgaacaa aaaccctggt tagctgacga atcgacaaag
     4141 acgacactcg tgacacagtc tgtcaaaatg atccaaaaga ttgcttacca cctaattatt
     4201 tttatgccga gcataggcga acaactatta aatcattttt cccaaccaac catattgccg
     4261 cttaccccat tatttccaag attagccaca ccaaaaaatc atgtctaatt ccatcaaacg
     4321 ctattttcga cggcaccccc ttcgcaccca acgaggtttg gaaatgttac ctgggatatt
     4381 ttcttggacg ctcattctct ttccaatttg gggttcgttc ctagtaccca acttagtcgc
     4441 gtactatgtc atcacttttg cagtctactg ggtatttcga tcattttcac ttgccacact
     4501 ggctctatct gctcatttca ctattaaagc atctagcaaa tatgattgga ttaaagacct
     4561 gaaccaacac tatggttcaa cctggcgcaa ggtacaccac ctaataataa ttcccactta
     4621 ccaagaacca cttgaaactt tggaaagaac tctccaatca ctttctcaac aaactatacc
     4681 gcttgctaat attcacatca tgctttcgtt tgaagagcgt gagggcgatg ccgcgaaaca
     4741 aaaagccaga tatctaacaa aaaaattctc aaatacattt ggcaacgtgt ggaccacgct
     4801 tcatccagac ataaaggatg aagtgaaggg caaatcagcc aacactaatt ggggcgctca
     4861 acgagcgaat gaatttatag taaataaccc atcgattaat tcagatttgg ttactgttac
     4921 gagcgaagat gccgacgcct gttttcatcc atcctacttt gctaatgtta cctttgattt
     4981 tctcagtgcc gaaaagccct acaaccttat ttggcaggga gccattgttt tttacaataa
     5041 tatttggaaa gtaccagcgc caattcgcgt gattgctgct gttttctctg tcacccaaat
     5101 gtatattctt aaacgttccg accgtttaat taatttttcg acctacacca ccacactgcg
     5161 tcacattcgc gaaattaatt tttgggatac cgacgttatt ccggaagact atcgcttatt
     5221 ctttaagtcg tactttgcaa agaagggtca attttgggcc cgaccaatct ttttgccaat
     5281 ctatgcagat gcggctgaag caaaaggcat gaagcaaaca tttatcaatc aatatgaaca
     5341 actcaagcgc tgggcatggg gcgttagtga tgatgcatat atcattcgtc agtttgtctt
     5401 gtctgatgaa ataccatttt gggataaaac aattagagtc ttaaaaacca ttgaggatca
     5461 ctttctttgg ccagtcaatt ggtttgcgat tactattgca gcgtttttcc cccactgttg
     5521 aatccagaat ttggtcgtac cattttagga aaaaccctac ctc
//
LOCUS       DAOQZP010000053         5460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_15230_length_5460_cov_4.229417, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000053 DAOQZP010000000
VERSION     DAOQZP010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15230_length_5460_cov_4.229417"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PKH60_04140"
     CDS             <1..377
                     /locus_tag="PKH60_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ17263.1"
                     /translation="LNLIVANQFVAQIDEEVKNAVFGNVGTMVSFRVGVPDANFLQHE
                     FAPTFNEIDLTNIEKYHVYIKTIVSNEPVPPFSMSLAKDMDKVKARMNPKQAEMIKQL
                     SRLKYGKDKEEVEREIQSRSNL"
     gene            386..2083
                     /locus_tag="PKH60_04145"
     CDS             386..2083
                     /locus_tag="PKH60_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HNQ17264.1"
                     /translation="MSIEIAALQQILLQSGSIKPEELSKWVEEAKSNKRDLLSVLIEE
                     QLYKPDDLGALLAKYFQVPYVDLRNKEIDQGALEQIPPEVAKRRRAICYRHDTKEGLW
                     VASDNPQANRLFIQLLAKKNSMSAQLAYTLPDSVDQALTHYTIDFQKYFERFLRDDDG
                     FVIRTTVHDPPVKKLVDQLIEIAHEQNASDIHIEPREDDFLVRFRIDGMLHDIFTLPK
                     YLLDRVVTRIKVLSRLRTDQHLAAQDGKMTVMIQDDRLDLRISVIPVAEGEKVVARLL
                     TSKARSYSLEDLGFSDSALERIQAAYKQSYGMILTTGPTGSGKTTSMYSVLKLVNTRE
                     RNLTSIEDPIEYRLPGANQVQVNTKTNLTFAAGLRSLLRQDPDVIFVGEIRDNETASI
                     AVNAALTGHLVLSTLHTNNAATTLPRLFDMEVEPFLVASTVRVIIGQRLVRKICVHCQ
                     QEEEIDSKEFLKQLFIDTLPANVEKKSGKITISKGAGCRFCHQTGYSGRIGIYEVLAV
                     SREIRDLVSARADSDVIHAQAVKEGMVTMFEDGVEKILQGLTTIDEILRVTRTEEVK"
     gene            2083..3153
                     /locus_tag="PKH60_04150"
     CDS             2083..3153
                     /locus_tag="PKH60_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNQ17265.1"
                     /translation="MSELKKVNTNSIRISAEEKISLFSNMATMLASGIAISEAIDSLL
                     DEAKGGRRTVLLTLQEDVGSGHHIHQSFAKFPKVFDKVTINLIKSAEESGTLETSLKD
                     IQANMQKELEFADKVKSALMYPSLVMVVFVLVLIVMLVVVVPKISQVFSRLKVDLPLA
                     TQVLIVMSNALTGQPLLTIGTLVLIVTGAVLLYRYKREAFHNFLYDMPGVSQLIRKTD
                     LTHFTRNLYLLLSSGIPIVMALELAEDVVRKSEVKALINQAKNRAVSGYPFFQGLRSK
                     DRIVPEMMIKLMEVGEKTGTLETAMQAVSEHMDYEVTKSLAKFTTILEPVMLVFVALV
                     VGGMMMAIISPIYGLISQVGVM"
     gene            3156..3665
                     /locus_tag="PKH60_04155"
     CDS             3156..3665
                     /locus_tag="PKH60_04155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNQ17266.1"
                     /translation="MKRAITKRQTVLTKKGFTMVELLLTMSILAILFTVVTTSLSTVI
                     SKSNIQSAAEILIADMREQQIKAMAGYELITGGGTDYGVMVFSDKYTLFSGDSYIEGM
                     DDNFTVNLQPGLEFDTSSLPFSMVVFERMSGEVASFNDSLHTIIIRNPTTQESRSLVF
                     NSLGVVELL"
     gene            3662..>5460
                     /locus_tag="PKH60_04160"
     CDS             3662..>5460
                     /locus_tag="PKH60_04160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNQ17267.1"
                     /translation="MIKIGSSQHSCGHTLIELLVALAVLGMLLPTLLASLSFSSQNHT
                     QQQSRQRAQAILIQTQEAMRSIRAAGWANVSSPGVFHPILEAGEWRLESGVGEDQGFT
                     TTITLSAVRRSDQGEIIDTGGWVDPSTMKVDIVVSWLLPQEASVTSTSYFTRYLDNQQ
                     LVETSEAHFLAGTLTATAVTNTNGGEVILGAGGQGDWCKPNQFILDELDLPGSGAARD
                     VEAIEGKAFTGTNAGSGTYAEISISNELPPVANIEALMTGYDTNDVYIDGSYAYVATQ
                     DVSRDVIIIDLSNNQEVGYFNDPDWIGSAQGVYVRGNVGYVTIGPRLHTFDLSQKTGS
                     RSQLDSVQLEGGFFWFLAQGYRLQVVGDYAYVAVNIGSAELRLINIANPSDIRRAARA
                     DVNGEQGKEVFVNETGTRAYLATSASSSKRELFVINTGVSTSNKNNSSYYLPVLSSYE
                     ANGMSPRGIKVVPGNRAILVGEGGEEYQVLDTSNELNIVRCGGYNTDTGIFGVAAVLE
                     SDEDAYSYIVTASPSAEFKIIQGGPGASVAAEGWFESNILDTGHDSGFNYLDFDFQLP
                     DVTVLEMQVSGADAVSGNCDLATFTYVGPDKTS"
BASE COUNT         1667 a          962 c         1296 g         1535 t
ORIGIN      
        1 gtcttaatct gattgtggcc aatcagtttg ttgctcagat tgacgaagaa gtcaaaaacg
       61 cagtatttgg caacgtggga accatggtgt cctttagagt tggtgttcca gatgcaaact
      121 ttttacaaca tgagtttgct ccaaccttta atgagattga tctaaccaat attgaaaagt
      181 atcatgtgta catcaaaaca attgtgagta acgaaccagt accacctttt agcatgtctt
      241 tggcaaaaga tatggataaa gtgaaggctc ggatgaatcc caagcaagca gaaatgataa
      301 aacagttatc aagattgaaa tatggaaagg ataaggaaga ggttgaacga gaaattcaat
      361 caagaagtaa tttataacgc ctgtcatgag tatagaaatt gccgccctcc aacaaattct
      421 tctacaatct ggatccatta agcctgagga gttatccaaa tgggttgaag aagcaaaaag
      481 caataaacga gatttgctca gcgttcttat tgaggagcaa ttatataaac cagatgatct
      541 tggggcgtta ttagcaaaat attttcaagt tccctatgtg gatttaagaa ataaagagat
      601 cgaccagggt gctcttgagc aaattcctcc agaagtagca aagcgaagac gagcaatttg
      661 ttatcggcat gatacaaaag aaggcttgtg ggttgcatca gataacccac aagcaaatcg
      721 cttatttatt cagttattag ccaaaaagaa ttcaatgtcg gctcaactgg cgtatacgtt
      781 gccagattca gttgaccaag ccttaacgca ctacaccatt gattttcaaa agtattttga
      841 gcgatttcta cgggacgacg atgggtttgt cattcgtacg acagttcatg atcctccggt
      901 aaagaaattg gttgatcaat taattgaaat cgctcacgag cagaatgcct cagatattca
      961 tattgaacca agagaagacg attttttggt tcgattccgt attgatggta tgcttcatga
     1021 tatttttacg ttacctaagt atttattgga cagagtggta acaagaatta aagtgctttc
     1081 tcggctgagg acagatcagc atttggcagc tcaagacggc aagatgaccg tgatgattca
     1141 ggacgaccgc ttggacttgc gtatttcagt gattccagtc gccgaaggag aaaaagtggt
     1201 ggcacgttta ttaacctcta aggcgcggtc gtattcccta gaagatcttg ggtttagcga
     1261 ttctgcgctt gagagaattc aagctgcgta taaacaatcc tacggcatga ttttaacaac
     1321 tggtcccact ggctccggta aaacaacctc aatgtattct gtcttgaagt tagtaaatac
     1381 tcgagagcgc aacttaacaa gcatcgagga tccaattgaa tacagattac caggcgcaaa
     1441 tcaggtgcaa gtaaatacta agactaattt gacatttgca gctggtttgc gctcgttgct
     1501 cagacaagat cctgatgtaa tttttgttgg tgaaattcgt gataatgaaa cggcatcaat
     1561 tgcagttaat gctgcactga cgggtcactt ggtgttgtcc actttacaca ccaacaatgc
     1621 ggcaaccacg ctgcctcggt tattcgatat ggaggttgag ccatttttgg tagcatcaac
     1681 ggtgagagta attattggtc agcgtctggt aagaaaaatt tgtgtacatt gtcagcaaga
     1741 agaagaaatt gacagtaaag aatttttaaa acaattgttt attgatactt tgccagcaaa
     1801 tgtggaaaaa aagtctggca aaatcaccat tagtaaagga gctggttgtc gattttgcca
     1861 tcaaacaggg tattctggtc gaattggaat ctatgaagtt ctggctgtat ccagagaaat
     1921 tagagattta gtttctgcgc gagcagattc tgatgttata cacgcccaag cggttaaaga
     1981 aggaatggtg acgatgtttg aagacggcgt agaaaaaatc ttacaaggct tgacaaccat
     2041 agatgaaatt ttgcgcgtaa ctagaacaga ggaagtgaaa taatgagtga gttaaaaaaa
     2101 gtgaatacta actcaattcg catttctgct gaagaaaaaa tttctctttt tagcaatatg
     2161 gctactatgc ttgcctctgg aattgctatt tctgaggcaa ttgattcgtt gctagatgaa
     2221 gcgaaaggag gcaggagaac ggtactgctc acattgcaag aagatgtggg gtctggccat
     2281 catattcatc aatcatttgc aaaatttccc aaggtgtttg acaaagttac gataaattta
     2341 atcaagtctg cagaagagtc tggaacttta gaaacatcac tgaaagatat tcaggcaaac
     2401 atgcagaaag aattggaatt tgcagacaaa gttaaatcgg cactgatgta tccatcgcta
     2461 gtcatggtgg tgtttgtgct ggtactaatt gtaatgctgg tggtggtagt accaaagatt
     2521 tctcaagttt tttcaagact aaaggtggac ttgccactcg caacccaagt cttgattgtg
     2581 atgtcaaatg ccctcacggg tcagcctctg ttaacaattg gaactttggt gttgattgtt
     2641 acgggcgccg tcttgcttta tcgttataaa cgagaggcat ttcacaactt tttgtatgac
     2701 atgccaggtg tatcgcaatt aattcgaaaa actgacttaa ctcactttac gagaaatttg
     2761 tatcttttgt tatcctctgg tattcccatc gtgatggcat tagaactagc tgaggatgtg
     2821 gtaagaaaat ctgaagtaaa agctttgatt aaccaagcaa aaaaccgcgc agtctctggg
     2881 tacccgtttt ttcagggctt gcgcagtaaa gacagaatcg tgccagaaat gatgatcaag
     2941 ctcatggaag ttggtgaaaa aacgggcact ctggaaaccg ccatgcaggc ggtttccgag
     3001 cacatggatt acgaagtaac aaaaagtctg gcaaaattta caactattct tgagccagtc
     3061 atgctggttt tcgttgcgct ggtggttggt ggcatgatga tggcaattat ctctccgata
     3121 tacggactta tcagtcaggt tggagtaatg tagtgatgaa aagagctatt acaaaaaggc
     3181 agacagtttt aacaaaaaag ggtttcacga tggttgagtt gctactcacc atgtcaattc
     3241 tggcgatctt gtttacggta gtaacaacga gtctttcaac ggtaatatca aaatcaaaca
     3301 ttcaatcagc tgcagaaatt ctcattgcag atatgagaga acaacaaata aaagcgatgg
     3361 caggatatga attaattact ggaggaggga ctgattatgg ggtgatggta ttttcagata
     3421 aatacacact tttctctggc gatagttaca ttgaaggcat ggatgataat tttactgtaa
     3481 acctacagcc gggactggag tttgatactt ctagccttcc attttcaatg gttgtttttg
     3541 aaagaatgag tggtgaagtt gctagtttca atgactcgtt acatacaatt attattcgga
     3601 acccaactac gcaggagtcg cgcagtttgg tgtttaattc gctgggggtg gtcgaacttc
     3661 tatgataaaa ataggaagct cccaacactc gtgtggtcac acattaattg aattgttggt
     3721 tgctttggca gtactcggaa tgttattgcc aactttgctg gcgagtttat ctttttcgag
     3781 tcaaaaccac actcagcaac agtctcgcca gcgagcccaa gcaatactca ttcaaacaca
     3841 ggaagcaatg agaagcattc gtgctgcagg gtgggccaat gtatcaagtc cgggggtgtt
     3901 tcatcctatt ttggaagctg gtgagtggcg tctagagtcg ggagttggtg aggatcaagg
     3961 gttcacaacc accattactc tatcagcagt gagaaggtca gaccaaggag aaataataga
     4021 tactggagga tgggttgatc cttcaaccat gaaagttgac attgttgtaa gttggctact
     4081 tcctcaagaa gcttcggtta caagtacctc atacttcacc cgatacctcg acaatcaaca
     4141 acttgttgaa acatcagaag cgcattttct agcaggaacc ctaactgcta cggcagtaac
     4201 aaacacaaat ggaggcgaag tcattctggg tgcaggagga cagggtgatt ggtgtaagcc
     4261 taatcagttt attttagatg aattagactt accaggatcc ggagctgcta gagatgtgga
     4321 agcaattgaa ggcaaggcat ttactggaac aaatgctggg tctggcacgt atgcagaaat
     4381 aagtatttca aatgaattgc ctccggtagc aaatatagaa gcattaatga caggatatga
     4441 tacaaatgat gtatatattg atggtagtta tgcgtatgta gcaactcagg atgtttctcg
     4501 agacgtgatt attattgatt taagcaacaa tcaagaagtg gggtatttta atgatccaga
     4561 ttggataggg tcagctcagg gagtttatgt acgaggtaat gtgggctatg tcacaatagg
     4621 gccacgactt catacatttg acttgagtca aaaaacaggt tcaagatcac aattagacag
     4681 tgttcagttg gagggaggct tcttttggtt ccttgcccaa ggataccgat tacaagtggt
     4741 aggtgattat gcctacgttg cagtgaatat tgggtcggct gagcttagac taatcaacat
     4801 tgcaaaccca agtgatatta ggagagctgc tcgtgctgat gtgaatggcg aacagggaaa
     4861 agaagtgttt gttaatgaga ccggcacccg agcatactta gcgacgagtg catcgagtag
     4921 caagcgggag ctttttgtaa taaatactgg agtatctact agcaataaaa ataatagttc
     4981 gtactatttg ccggtattgt cgtcttatga agcaaatggc atgagcccca gaggcataaa
     5041 agtcgtacca ggaaatcgag caattctagt tggagagggg ggagaagaat accaagtgct
     5101 agatacaagt aatgagctca acatcgtgcg atgtggtggt tacaatactg atacgggcat
     5161 ttttggtgtt gcagctgttc ttgaatccga tgaagatgca tattcatata tcgtaacagc
     5221 gagtccgtcg gcagaattta aaattattca gggtggacca ggggcatccg tagcagcaga
     5281 gggatggttt gagtcaaaca ttttggatac gggacatgat agtgggttca attatttgga
     5341 ttttgatttt caactccccg atgttactgt gctagaaatg caagtttctg gtgctgatgc
     5401 ggttagtggt aattgtgatc tcgccacatt tacctatgtt ggtccagaca aaactagcaa
//
LOCUS       DAOQZP010000054         5371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_15563_length_5371_cov_5.398796, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000054 DAOQZP010000000
VERSION     DAOQZP010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15563_length_5371_cov_5.398796"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PKH60_04165"
     CDS             <1..264
                     /locus_tag="PKH60_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNQ17268.1"
                     /translation="FFLTKTCRPFLVRNIRTALDRYFRMAGIKEAKVNDLRHTFVAEQ
                     LKAGTPLVYVSQLVGHKRITTTEKYLKLIDAPEMEPNVQIEEL"
     gene            291..1697
                     /locus_tag="PKH60_04170"
     CDS             291..1697
                     /locus_tag="PKH60_04170"
                     /inference="COORDINATES: protein motif:HMM:NF027967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNQ17269.1"
                     /translation="MQFNLKLMSFIFVSVFLPLLFLFSLISKSNADGGVRLDSSSLRV
                     GTITELSATIAGSQCAATTRQFDRLSINADFLENPLSTIQFASPRVVLKINQTVVPGH
                     IYQNYGTVIHWRPDSGSMMLSNMDLLEIKVNGVDILEPNLVGFAFEFYNQGLPLNDGP
                     ETCFGWGYYSYSTPCDEFYSGCFIKRFSESGIQPTPTLGPTTAPSITTTPVPTHLPTP
                     TASPTTRPTATPTATPTPTPSAISMTELGFTEQGPIEVSIVPGQTVPVFTLTSVSASQ
                     FGMYGYPTSYGPGINITPASGGLFPGMSTLISIQVTSTVPLGTYNGLQRVTAGGTYPY
                     ALIPVTVHVVESLPTATPTPTQLPTATPLPTNTPTPVPTPVPLSISNVVAANISARAA
                     QIRWSTSVAATSFVQYGTKLNAMNSYTTEGTNQSVLHQVDLINLSPNKKYYYKAHSRN
                     SLGEWATSEVGSFTTPRR"
     gene            1838..2086
                     /locus_tag="PKH60_04175"
     CDS             1838..2086
                     /locus_tag="PKH60_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HNQ17270.1"
                     /translation="MIRTQVYLPDKLFHQAKARAAATNSTISQLIRDGLLVALSNQSG
                     QLKETTGHFLLKNFAGQGRGKKGVEAALHHDDIYELEK"
     gene            2092..2487
                     /locus_tag="PKH60_04180"
     CDS             2092..2487
                     /locus_tag="PKH60_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNQ17271.1"
                     /translation="MFVLDTDFIIALRFPQESTHQRAVTYAKKYLKESESFVTDLVQV
                     ELANVISRKYSQEQAIQAIRMIQKAPNGTLYLDKENISSSWELFFQQKKRGMSVVDCS
                     NTIVAKKLGFQIVSFDTFYREFDLLVAGM"
     gene            2504..3463
                     /locus_tag="PKH60_04185"
     CDS             2504..3463
                     /locus_tag="PKH60_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17272.1"
                     /translation="MKLVKEKPPSLADRRSFLRFASRIFVGFVATSVVKSAVGEPQTL
                     ESQRELELQNLDWYSQWINEGRSVRLVDDQMALSELDKLELVDVDLTQGLAAVDHPLK
                     YAQQIPQLIQNFQNDSLVEQAWVAPMRGGWTITVNRYANLTDLDSQYENLKQNAVKPL
                     DYPDLLKDWSNKSVVTFSKTVGDYEIVWSISNTGGQSNPLLQGGRVERGDYTLSITSK
                     MSGERIAAVGFVGRLIESGLDVQHAFKNVGLHSLKRYADPKTGSSMAYFNFGSLHPPG
                     SFPLENTHHTMASLDEKQINVLVAAILAFVSTTVSPQIAVTTV"
     gene            3487..4485
                     /gene="trpS"
                     /locus_tag="PKH60_04190"
     CDS             3487..4485
                     /gene="trpS"
                     /locus_tag="PKH60_04190"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNQ17273.1"
                     /translation="MKKRIVSGITPSGNSLHIGNYFGAVKPQIELQNNEEFETFYFVA
                     DLHALTTVQNPTMLSDNVHNVVLDYLALGLKYEKSVFFRQSQSVGHSQLATVLANYIG
                     LGQMKRMHAYKDKLQKGTDVDTVNMGLFNYPILMAADILLYKPYGVPVGEDQRQHVEL
                     TRDIAENFNKTYGQEIFPLPEPLISKEVGKIVGTDGERKMSKSLENTIGIFEDESIIK
                     KQIMSCFTDPNRLKATDPGKVEGNPVFIYHDLLNENKAEVEELKNRYRLGQVGDVEVK
                     EKLFLAHQAYFATARASRKALAEMPEKIVEILRAGASRSSDVGNQTMTEVYSLIGL"
     gene            4499..4843
                     /locus_tag="PKH60_04195"
     CDS             4499..4843
                     /locus_tag="PKH60_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HNQ17274.1"
                     /translation="MKDLISYDDFVKLDIRIATVIAAEPIEGADKLLKLTLDVGDPET
                     GGLGQRTIAAGIKAWYQPEDLIGKQIVYLANLEPRMLRGVESQGMVLAADGEQAVLLQ
                     PWANVSNGSMIR"
     gene            4877..>5371
                     /locus_tag="PKH60_04200"
     CDS             4877..>5371
                     /locus_tag="PKH60_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HNQ17275.1"
                     /translation="MEEKKEEKKTQNKQSNQLKNTVVKKIELKLNFQQIVNRGFLYVL
                     IGILFLPFLLGLFGSNREEITLSQLVKDLRAEKIEKIAIAGDELRASYKDDEVERFAR
                     KEAGVSTSQIFANADIDLAQADLKVENISGWQLLFEAAINFLPVILMIVFFFFIFRQA
                     RGGAD"
BASE COUNT         1673 a         1023 c         1138 g         1537 t
ORIGIN      
        1 ttctttttga ccaaaacctg cagaccattt ttggtgagaa acattagaac tgccttagat
       61 agatatttcc gcatggcagg catcaaagag gcaaaggtca atgaccttcg ccataccttt
      121 gtggccgaac aattgaaagc tgggactcca ttagtctatg tctctcaatt agttggtcac
      181 aaacgcatta ccactaccga aaaatatctg aaattaattg acgctcccga aatggagccc
      241 aatgttcaga ttgaagagct gtaataccat atttagcaag ggagcacgct atgcagttta
      301 atctcaaatt aatgagtttt atttttgtga gcgtgttttt accgttatta ttcctttttt
      361 cattaatttc aaaatctaat gccgatggag gagttcggct ggacagctca agcttaagag
      421 ttggtacgat aactgaattg agtgctacta ttgcaggaag tcaatgcgct gctacaacaa
      481 ggcaatttga tcgattaagc atcaatgccg actttcttga aaatccactt tcaacaatac
      541 aatttgcatc tccaagagtt gtgcttaaaa taaatcagac agtggttcct ggtcatattt
      601 atcaaaacta tggaactgtg atccactgga gaccagattc aggttcaatg atgttaagca
      661 acatggattt actagaaatt aaagtcaatg gtgtagatat tcttgagccc aatctagttg
      721 gttttgcctt cgaattttat aatcagggtt taccgttgaa tgacggaccg gaaacttgtt
      781 ttggatgggg atactattca tattccacac cttgtgatga attttactcc ggatgtttta
      841 tcaaaagatt ctctgaatct ggtatccagc caactcccac tctcggacca acaacagcgc
      901 caagtataac aactacaccc gtaccaactc acttgccaac gcccaccgct agtccaacca
      961 caaggcccac tgctacacca acagctacgc caaccccaac accttcggcg atttcaatga
     1021 ccgaacttgg gtttactgaa caaggaccaa ttgaggtatc tattgttcca ggacagactg
     1081 taccagtatt tactcttaca tctgtttcag ctagtcagtt tggtatgtac ggatatccta
     1141 cttcttatgg tccaggtatt aatatcacgc cagcatcagg cggtctgttt cctggaatga
     1201 gtacgcttat tagtatccaa gttacgagta ctgttccgct tggtacctac aatggcttac
     1261 aaagagtaac tgcgggtggt acttatccgt acgcactgat tcctgttacc gtccatgtag
     1321 ttgagtcatt acccaccgct acaccaacgc caacacaact accaacagct acgccgttac
     1381 caaccaatac accaacgcca gttccaactc cagtaccttt gagcatcagt aatgttgttg
     1441 cagcaaatat ttccgccaga gctgcgcaga ttcgatggtc tactagtgtc gctgcaactt
     1501 catttgtaca atatggcaca aagcttaatg cgatgaatag ctatacaact gaaggtacta
     1561 atcaatccgt tctccatcaa gttgatttaa ttaacttaag cccaaataaa aaatattatt
     1621 acaaggcaca ttcaagaaat agtcttggtg aatgggctac ttctgaagtt ggatctttta
     1681 ccactccaag gcgttaatga gcactacgcc agattggaga gctttaaata atactaacaa
     1741 catgttgcgg ttgttgtaag gttttcattt tattgtatgt gtagaaagtt ctatgaatac
     1801 attgacaaca tacaacatac aacatacaat gatgggcatg attagaacac aagtctactt
     1861 gccagataaa cttttccatc aagccaaagc ccgtgcggct gcaacaaact ctacaatttc
     1921 tcaactgatt agagatggtc tcctagtagc cctatccaat cagtcaggcc aactcaaaga
     1981 aacaacgggg cactttctct tgaagaactt tgctggccaa ggtagaggca aaaaaggtgt
     2041 agaagcagct ttgcatcatg atgatatata tgaacttgaa aaatagaaga tatgtttgtt
     2101 ttggatactg attttattat tgcccttcgt tttcctcagg agtcaacgca ccaacgagca
     2161 gttacttacg cgaaaaaata tctaaaagag tcggaaagtt ttgttaccga tttagttcaa
     2221 gttgagttgg cgaatgtaat tagtcgaaag tattctcaag aacaagccat acaagcaatt
     2281 cgaatgatac aaaaagctcc caatggaact ctctatttgg ataaagaaaa tattagcagc
     2341 tcttgggagt tattttttca acagaaaaaa cgtggtatgt cagtagttga ttgttccaat
     2401 acaattgttg ccaaaaaact gggcttccaa atcgtgtctt ttgatacatt ttatcgagaa
     2461 tttgatttgc tagtcgctgg tatgtgagta ttcagagaaa ataatgaaac tagtgaaaga
     2521 aaaaccacct agtttggcag atagacgatc gtttttgagg tttgctagta ggatttttgt
     2581 aggttttgtt gcaacctcag tagtaaaaag tgctgttgga gagcctcaaa cgctagagag
     2641 tcagcgggaa ctagaattac aaaatcttga ttggtatagc cagtggatca atgaaggtag
     2701 aagtgttcgg ttggtggatg atcagatggc tttgagcgag ctcgataagc ttgagttggt
     2761 agatgtcgac ctgacacaag gtttggctgc ggtagaccac ccgctcaagt atgctcagca
     2821 aattccacag ttgattcaga actttcaaaa cgatagttta gtggagcaag cttgggtagc
     2881 tcccatgagg ggaggttgga ctataacagt gaatagatat gcaaatctga ctgatttaga
     2941 ttcacagtat gaaaacctca aacaaaatgc tgtaaaaccg cttgattacc cagatttgct
     3001 taaagactgg agtaacaaaa gtgttgtcac tttttcaaaa actgttggcg attatgaaat
     3061 tgtttggtca atttcgaaca ctgggggtca gagcaatccc ttattacaag gaggcagagt
     3121 tgagcgggga gattatacat tgagcatcac ttcaaaaatg tctggagaaa gaattgctgc
     3181 agtaggcttt gtaggtcgat taattgagtc aggacttgat gtgcagcatg cattcaaaaa
     3241 cgttggttta cattcactga aaaggtatgc agacccaaaa actgggtcca gtatggctta
     3301 ttttaatttt ggctcgttgc atccgccagg aagttttccg ctcgaaaaca cacatcatac
     3361 aatggcttca ttagacgaaa aacaaatcaa cgtgctagtt gctgccatac tagcgtttgt
     3421 aagtactaca gtttccccgc agatagcagt aactacggta taatcgataa cgattaagga
     3481 ttatctatga aaaaacgtat cgtatctggt attactccga gtggcaactc gctgcatatt
     3541 ggcaattatt ttggtgctgt gaagcctcaa attgagttac aaaataatga ggaatttgag
     3601 actttctatt ttgtagcgga tctgcacgct cttacaacgg tgcaaaatcc aaccatgttg
     3661 tctgataatg ttcataatgt tgtactagac tatttggctc ttggtctcaa atatgaaaaa
     3721 tctgtatttt ttcgccaatc acaatcggtg gggcattctc agcttgcaac agttttggcc
     3781 aactacattg gtttgggtca gatgaaacgc atgcacgcgt ataaagataa actccaaaaa
     3841 ggtacagacg ttgataccgt gaatatggga ttattcaact atccaatttt aatggcagca
     3901 gatattttgt tgtataagcc atatggtgtg ccagtaggag aagaccagcg tcaacatgtg
     3961 gaattaacga gagacattgc cgaaaatttc aacaaaacct atggtcaaga aatctttcct
     4021 ttaccagagc cacttatttc taaagaagtc ggaaaaatcg ttggcaccga tggcgaacgc
     4081 aaaatgagta aatctttgga aaatacgatt ggtatttttg aagatgaatc catcattaaa
     4141 aagcaaatca tgagttgttt tacagacccc aatcgactga aagcaaccga tccaggtaaa
     4201 gtagagggaa atccagtgtt tatctatcac gatttgttga atgagaataa agctgaagtt
     4261 gaagagctta aaaatcgata tcggttgggt caagtgggtg atgtggaagt taaggaaaag
     4321 ctttttttag cacatcaagc ttattttgct actgctagag ccagtcggaa agcattggca
     4381 gaaatgccag aaaaaatagt cgaaatactt cgcgctggag ctagtcgatc aagcgatgtt
     4441 ggtaatcaaa ccatgactga agtgtattca ctcattggct tatagtaagg aaaaattgat
     4501 gaaagaccta attagttacg atgatttcgt gaagcttgat attagaattg caactgtaat
     4561 cgctgctgag ccaatcgaag gtgccgataa attactcaaa ctcacacttg atgttggtga
     4621 tcctgagact ggaggattgg gccaacgaac catagcggcg gggataaaag cctggtatca
     4681 gcccgaagat ttaataggta aacaaattgt ttacttagcc aatttggagc cgagaatgct
     4741 taggggtgta gagagtcagg gaatggttct agccgcagat ggggagcaag cggtgctttt
     4801 gcagccttgg gcaaatgtct caaacggctc gatgatcaga taaaattcaa ttctccgtgc
     4861 tacaataacc gccgttatgg aagaaaaaaa agaagaaaaa aaaacacaga ataaacagtc
     4921 taatcagtta aaaaatactg tagtcaagaa aatcgagctc aaactaaact ttcaacaaat
     4981 agttaatcgt ggatttttat acgtactcat tggtatttta ttcttaccgt ttttgttggg
     5041 actttttggc tcaaacaggg aagaaattac tctgagccaa ttagtcaaag atctcagggc
     5101 agaaaaaatt gaaaaaattg ctattgccgg tgatgagtta cgagccagtt ataaagatga
     5161 tgaagttgaa cgctttgctc gaaaagaagc aggtgtttcg accagtcaga tctttgccaa
     5221 tgccgacatt gatcttgctc aagctgattt gaaggtcgag aacatttcgg ggtggcagtt
     5281 attattcgaa gcagcgatta actttttgcc agtcatcctt atgattgtgt tctttttctt
     5341 tatttttcgc caagcccgag gtggggctga t
//
LOCUS       DAOQZP010000055         5202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_16222_length_5202_cov_6.123179, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000055 DAOQZP010000000
VERSION     DAOQZP010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16222_length_5202_cov_6.123179"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..74)
                     /locus_tag="PKH60_04205"
     tRNA            complement(6..74)
                     /locus_tag="PKH60_04205"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42..44),aa:Ile,seq:cat)
     gene            176..249
                     /locus_tag="PKH60_04210"
     tRNA            176..249
                     /locus_tag="PKH60_04210"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:210..212,aa:Met,seq:cat)
     gene            complement(250..876)
                     /locus_tag="PKH60_04215"
     CDS             complement(250..876)
                     /locus_tag="PKH60_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17276.1"
                     /translation="MTINSIYIKYQIPPNLQRHMRDVASVCLLILENWIGETVDQQLA
                     IRSALLHDLGNIVKFKRPFMGDLEDEAERWQNVQDEIMSTYGADAKKATHKMVEELGH
                     NNTIGKVLHDMDLLIEGVSNVMTEAKIVEFADLCVSPAGIVGYERRKQDLIDRYGATH
                     GLGWVEPAERLLLTLQKNVDIDLTTIETYDFSEYGKIVSQIVLEDEIL"
     gene            complement(1139..1414)
                     /locus_tag="PKH60_04220"
     CDS             complement(1139..1414)
                     /locus_tag="PKH60_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17277.1"
                     /translation="MNNQSLSATTVKINVPTGMLQNARMESQRIGISVQDFIRMLLAT
                     YFAHAPSIKAITSDDERYQRALEDISSGKFSMVKSQKELLAHLRTLE"
     gene            complement(1554..3179)
                     /gene="groL"
                     /locus_tag="PKH60_04225"
     CDS             complement(1554..3179)
                     /gene="groL"
                     /locus_tag="PKH60_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008684543.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNQ17278.1"
                     /translation="MSAKQLVFSDDARQKMLAGINKLASAVTTTLGPKGRNVAIDRSW
                     GAPNVLHDGVSVAKEITLEDKFENMGAQLVKEAASKTNDVAGDGTTTATLLAQHIAGK
                     GMKYVTAGTNPMIMKKGIDRAVEAVVAEIRRLAKPVKESDWEKVATISAQNEVIGQKI
                     AEALRLVGKDGVVEVEEGKTLDITIEHKDGMEFDKGFASPYFVTDSDHMESVIENPYI
                     LVTDFKLSSIQDLLPMLEKLMTVSKNLVLIADDVEGEALTTLVVNKLRGAINVLAVKA
                     PGFGDRRKAMLQDIAVLIGANYISQDTGRQLKDVAVEDLGQADSVRASKDATRIVGGK
                     GKQTDIDARVAQIEAEIEKSTSDFDIEKLQERKAKLTGGVAVIQVGASTEIEMKNLQE
                     RVKDAKGATKAAIEEGIIPGGGVTLLQAVKVLDKLKSNTHDEQVGIDLIKAVVQEPIR
                     MLAINSGMDPGYVLHQVLGQNDPFYGFNALTNEFGDMVKAGVIEPAKVAIAALQNAAS
                     VGSMILTTECLVTDVPEEKKPMMPAGGGMDGMM"
     gene            complement(3208..3516)
                     /locus_tag="PKH60_04230"
     CDS             complement(3208..3516)
                     /locus_tag="PKH60_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010181546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNQ17279.1"
                     /translation="MSTTLSVKSISPLAGYVLVEPAKQEKQTASGLYLPDSADEKPQH
                     GTVLAVGDSIWESGVKELKSPVKVGDKVIYKKWGGNDFKIGDVEYQFLKFEDILATIK
                     "
     gene            complement(3629..4246)
                     /locus_tag="PKH60_04235"
     CDS             complement(3629..4246)
                     /locus_tag="PKH60_04235"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNQ17280.1"
                     /translation="MEFDRLVVSFLVDEVVGGFFISVPPGHLACVYDRGRGVLPRVWG
                     PGLHFKIPFWQVAKLFNAQVLEYSIRDGFNFEANNEALGDAPIHATTQDGKDVAVEGS
                     VLFRIDRKNAPELWENIGEDMVSKVVRPAARSKVATIFSSLTLDQIKHNRSHVEETIK
                     QEIQHYFEDKGLNCEGFLLSTVRQHAAGGVREILAPQVLVESTSS"
     gene            complement(4261..4569)
                     /locus_tag="PKH60_04240"
     CDS             complement(4261..4569)
                     /locus_tag="PKH60_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17281.1"
                     /translation="MDQKQRSYLQLTVRHREISAFRDVLRQVKDMPLKQKQRWLDANA
                     EAMQSAFSIFVDVSERTLDEMSQNNENMELSYQLVTTLREAEALVAEVFHQPSAQLQS
                     "
     gene            4621..>5202
                     /locus_tag="PKH60_04245"
     CDS             4621..>5202
                     /locus_tag="PKH60_04245"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNQ17282.1"
                     /translation="MKIVQLTQLDSKEVVTLTTEVLQAGGLVIYPTETVYGAGVDATN
                     QVAVDKLLQYKSRREGKPLSIAVSDRAMAEQFVEVNEQARQLYQRFLPGPMTVISQVK
                     PNSDKLARGVASEFNTLGIRISSHPFVEAMVTKFGKPFTATSANASGKRRPYSIAQVL
                     NHLSEKQRGLIDLIIDAGTLPNNPPSTVIDTTLS"
BASE COUNT         1403 a         1207 c         1018 g         1574 t
ORIGIN      
        1 tattgttgcg gggccgggag tcgaacccgg cctgtaagat tatgagcctc acgtgcaccg
       61 tacactaccc cgcgcactat cgacaacttg atcgatgacg tctgctattg taacagcata
      121 ggaagaaacg tcaattcttg gtataatcct gctttgccga aagaaaacgg catgtgccag
      181 ggtagctcag ttggttagag catgggattc ataaacccaa ggtcgagagt tcgaatctct
      241 cccctggtac taaagtattt catcctcaag tactatttgg gaaacgatct ttccatattc
      301 tgaaaaatca tatgtttcga tggtggtaag atcgatgtca acattttttt gtagagttaa
      361 aagaagtcgc tcagctggct cgacccaacc caaaccgtga gttgctccgt agcgatcgat
      421 caaatcctgc ttacgtcgtt catacccgac tattcccgcc ggagaaacac aaagatcggc
      481 aaactcaacg attttggcct ccgtcataac gttgctaaca ccctcgataa gtagatccat
      541 atcatgtaac actttgccaa tcgtattgtt gtggccgagt tcttcaacca ttttatgcgt
      601 ggcttttttt gcatctgccc catatgttga cataatctca tcttgaacat tctgccaacg
      661 ctcagcttca tcttctaaat ctcccataaa cggacgttta aatttaacaa tgttacccag
      721 gtcgtggagt agtgcagaac gaatcgctag ttgttggtcc actgtttccc cgatccagtt
      781 ttccaagatt aataagcaaa ctgacgcaac atctcgcatg tggcgttgaa ggttgggggg
      841 aatttgatat ttgatgtaaa ttgaattaat tgtcatcaat atacatcctc atgttttcct
      901 atatgaaata cagttacaca gtgtttctca aatataaaaa gtattctcac acttttatcc
      961 actgaaatcg acttgaagtt agatttttgt attttgtgca acctcagtga aggatgattt
     1021 ggattgacta ataacaattc cagttgtttt ttaattttct gtttaagtgg ttcatttctc
     1081 ctaattaatt ttgcatactc agcatcaaaa gtttttgtaa aacgaagctt catagcaatt
     1141 attcaagagt tcgcaagtga gctaacaact ctttttggga tttcaccata gaaaattttc
     1201 cacttgagat atcctctagc gctcgttggt atcgttcatc gtctgaagta atagctttta
     1261 tcgatggcgc atgagcaaaa tacgttgcta gtaacatgcg gataaaatct tgcacactga
     1321 taccaatccg ttgagattcc atacgggcat tttgtaacat accagttggt acattaatct
     1381 ttactgtagt ggccgataat gattgattgt tcatagtgaa ttgcatttta tagtaccagt
     1441 gtgtaccaag tcaagtctat acagggtata aatacatttt gataaacgaa aaacccagcc
     1501 tttcgcgaaa ggctgggttg atcttgtttc aattacattt gctcttcgac accttacatc
     1561 atgccatcca taccaccacc ggctggcatc ataggtttct tctcttctgg cacatcggtg
     1621 accaagcatt ctgtcgtgag gatcattgac ccaacactag cagcattctg caaggcagcg
     1681 atggctacct tggcgggctc aatcacgcca gctttgacca tgtcaccaaa ctcgttggtg
     1741 agggcgttaa agccgtaaaa ggggtcgttt tgccctagca cttggtgaag gacataacct
     1801 ggatccatac ctgaatttat ggccaacatt ctgatgggct cttggaccac tgctttgatc
     1861 aagtcaatgc caacttgctc atcgtgagtg ttagatttca gcttgtcgag cactttcaca
     1921 gcctggagta atgtcactcc accaccaggg atgatacctt cctcaattgc ggctttagtg
     1981 gcacccttcg catctttgac ccgttcttga aggtttttca tctcaatttc ggttgatgct
     2041 ccaacctgga tcacagctac cccaccggtt aacttggcct ttcgctcttg tagtttctca
     2101 atatcaaagt cagaggttga cttttcaatc tcggcttcga tttgagccac acgagcgtca
     2161 atatcggttt gtttgccctt gccacccaca attctagtgg catctttgct ggcgcgaaca
     2221 gaatctgctt ggccaagatc ctcaacggcc acatctttga gttggcgacc ggtgtcctga
     2281 gaaatatagt tagcgccaat caaaactgcg atatcctgca acatcgcttt gcgacggtcg
     2341 ccaaagccag gagccttcac agctaagaca ttaatggctc cgcgcaactt attcaccacc
     2401 aaagtggtaa gcgcttcgcc ttcaacatca tcggcaatca aaactaagtt tttgctgaca
     2461 gtcattaatt tctccaacat tggtagcaaa tcttgaatgc tgctcaactt gaagtcagtg
     2521 actaagatgt atggattttc gatgactgat tccatatgat ctgaatctgt cacgaaatag
     2581 ggagaggcaa aacccttgtc gaattccatt ccatccttat gttcgatggt aatatcgagg
     2641 gttttgccct cttcgacttc taccacacca tctttaccca ccaatcgcaa ggcttcggca
     2701 atcttttgac caatcacttc attctgggca gaaatggtgg caactttttc ccagtctgat
     2761 tctttgactg gcttagctag gcgacgaatt tctgccacta cagcttcaac cgcacgatca
     2821 atacccttct tcataatcat tgggttggtg cctgcagtca catacttcat accttttcct
     2881 gcaatatgtt gtgctaacaa agttgcagtt gtggtaccgt ccccagccac atcattagtt
     2941 ttgctggctg cctctttaac caattgggcg cccatgtttt caaacttgtc ttctaaagtg
     3001 atttccttag ccacgctcac tccgtcatgg agtacgttgg gggcgcccca agaacgatca
     3061 atcgctacat tacgtccctt aggacccaat gtagtggtga ctgccgatgc aagcttatta
     3121 atacctgcca acattttttg tctggcgtca tccgaaaata cgagttgttt tgctgacata
     3181 taatctcctt ttttaacttt ctaattatta tttgatggtt gctaaaatat cttcaaattt
     3241 taaaaattga tactcaacat cgccaatttt gaaatcatta ccaccccatt ttttataaat
     3301 tactttatcg cctactttaa cggggctttt taattctttg actccgcttt cccagattga
     3361 atcaccgaca gccaacaccg taccatgttg aggtttttcg tcagctgaat ctggcaagta
     3421 taagcctgat gcagtttgtt tttcttgttt ggctggttct actaaaacat accccgccag
     3481 aggtgaaatt gatttaaccg acaacgttgt ggacataaaa ttctccttac tactgaatac
     3541 tttttttagc agtttaaata atcaactgct actaaactag cagtgataat agtcgtctgc
     3601 taactatttg tcaacaacgg agttgcaatt atgaggatgt agattcaact aatacttgtg
     3661 gagccaggat ttctcgtact ccacccgcag catgttgacg aacggtggag agtaaaaatc
     3721 cttcgcagtt taagccctta tcttcaaaat agtgctgaat ttcttgttta atagtttcct
     3781 ctacgtgact gcgattatgc ttgatttgat caagtgttag actagaaaaa atcgtagcta
     3841 ctttacttct agcagccgga cgcactactt ttgaaaccat gtcttcacca atattttccc
     3901 aaagttccgg tgcattcttt cgatcaattc tgaaaagtac ggaaccctca actgccacat
     3961 ctttgccatc ttgagtggtg gcatgaattg gcgcatcacc aagcgcttcg ttattagctt
     4021 caaaattgaa tccatctcta atggaatatt caagtacttg cgcattgaaa agtttagcca
     4081 cttgccaaaa gggaatttta aaatgtaaac cgggacccca gactcttggc aagacacctc
     4141 tgcctcgatc atatacacac gctaaatgtc ctggtggcac gctaataaag aatccaccaa
     4201 caacttcatc aaccaaaaaa gatacaacca gacgatcaaa ttccataggt atattgtact
     4261 ctaactttgc agttgtgcag agggctgatg gaaaacttcg gctaccagcg cttcggcttc
     4321 tctcaaagtg gtaactaatt gatacgataa ctccatattt tcattatttt gcgacatttc
     4381 gtctaaagtt ctttctgata catccacaaa aatagaaaaa gccgattgca tggcttccgc
     4441 attggcatca agccaacgct gcttttgttt caaaggcata tctttgactt gcctgagcac
     4501 atctctaaaa gcagaaattt cacgatgacg tacagttaat tgtaaatagg aacgctgttt
     4561 ttggtccata ctcccattat agcagtagtt agccactgag tatgccctat aataccaggt
     4621 gtgaaaattg tacagctaac tcagctagat agtaaagaag tagtaacact gactactgag
     4681 gtattgcaag ctggtgggct ggtcatctat ccgaccgaaa cggtgtatgg cgccggcgtg
     4741 gatgcgacta atcaagtggc ggttgataaa ctattgcaat ataagtctcg tcgtgagggt
     4801 aaaccactgt ctattgcggt atcagatcga gcgatggcgg aacagtttgt tgaagtaaat
     4861 gagcaggcgc gccaactgta ccaacggttc ctaccaggac caatgacagt aatttctcag
     4921 gtcaaaccaa actcagataa gttggcgcgc ggcgtggcat cagaatttaa tactttgggt
     4981 attcgcattt ctagtcaccc ttttgttgaa gcgatggtaa ccaaatttgg caaacctttc
     5041 actgctactt cggctaacgc ctctggcaaa cgtcgcccct attccatcgc tcaagtactc
     5101 aatcatttat ctgaaaagca aagggggttg attgacttga ttatcgacgc cggcactttg
     5161 cctaataatc caccttcaac ggtgattgat acgacattat cc
//
LOCUS       DAOQZP010000056         5030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_16931_length_5030_cov_4.833970, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000056 DAOQZP010000000
VERSION     DAOQZP010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16931_length_5030_cov_4.833970"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..389)
                     /gene="secG"
                     /locus_tag="PKH60_04250"
     CDS             complement(174..389)
                     /gene="secG"
                     /locus_tag="PKH60_04250"
                     /inference="COORDINATES: protein motif:HMM:NF015779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNQ17283.1"
                     /translation="MQQVLLVVQIVLSIIITVSILLQAQGAGLGTAWGGGSETYHTKR
                     GLEKVLVYVTIGSIVLFTILSVASLLI"
     gene            complement(407..2020)
                     /gene="rny"
                     /locus_tag="PKH60_04255"
     CDS             complement(407..2020)
                     /gene="rny"
                     /locus_tag="PKH60_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNQ17284.1"
                     /translation="MSLFSNLSSLFASTDNKNQKQNKRVQSDVKSKTVVKPTPAQPPK
                     TSGVTQPPVVPIQPRVSTPLPDVSSLDVVREAQSKAREIIVEAKSEALTIRSKAENDA
                     RDVARQVEEQQRSLENKLDRIDARLEQLDSREKNLNLQQEKVAESQKKVDQLQQQAIK
                     EIENVAGLTSDEAKRELFSQLEKTSKREIAQFVRQKQEEAQAEAQEKVQEILVDAMKH
                     GATDYVSEYTVSVVELPNEEAKGKIIGKSGRNINSFEKETGVDVDLDMSPTEVRLSCF
                     DPVRREIARISLERLIKDGRIQPARIEETVEKVKSEIEKITLEAGKKLCHDVGIYNLP
                     IDLMKLLGKFKYRFSYGQNMIAHTLEETKIGMKLASELKLDVNVVKLGCLLHDIGKVS
                     EEAEGSHVELGVKIAKRFNIPQPVIDCIAQHHEDEPFSGPEQMVVYIADAISGARPGA
                     RYENYDEYVARLQKLEEIATAYNSVKSAYAIQAGREVRVLLKPQESQDDDVVILSRKI
                     RDEIKEKVTYPGTVTVTVIRETRSKEVAS"
     gene            complement(2297..3331)
                     /gene="recA"
                     /locus_tag="PKH60_04260"
     CDS             complement(2297..3331)
                     /gene="recA"
                     /locus_tag="PKH60_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNQ17285.1"
                     /translation="MTKPAKVTPTTEETGRSQAVVIAMQQIEKEYGKGSIMKMGDQLK
                     ELGTVPVVSTGSLSLNLALGVGGFPKGRIVEIYGPEASGKTTLCLHAIAEVQKQGGTA
                     AFIDVEHALDPVRAKLIGVNLDDLLISQPDYGEQALEIAETLIRSGGVDIVVIDSVAA
                     LVPKSEIEGEMGDHQMGTQARLMSQAMRKLTAAINKTKTLVIFTNQIRMKLGVMFGNP
                     ETTSGGNALKFYASVRLDIRKVGNIQEGEKIVGSRHRVRVKKNKMAPPFQVAEFDMDD
                     YGISITGDVLDIGVERNIVTKAGSFFKYEGEVLAQGREAAKQYLAEHPKTIEEIKQKI
                     WDVIRQERDK"
     gene            3458..4990
                     /locus_tag="PKH60_04265"
     CDS             3458..4990
                     /locus_tag="PKH60_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17286.1"
                     /translation="MNSPEGTPVPKELPADNLPSVEHSQPLKDREFWWQWLHAGERQI
                     RIKRTDKELLVGEASEFSESLSNQAMARILAELESDRLSPNKNIVDLPFIEWPEEVIT
                     ALRFRDGIGSLLDSLDDAEKNEVKFIDLCEVLIENEAKIKYGFTGRYWSPEKLSEAEI
                     FERSIAIGTYFLEQVLIQQKWGDDIDAVLVTSAVLPETISLEIINFAKQKGILKKRKR
                     IQARDIRTACSGVFFADVIAAKDKKIQKKKRAVILALEPLGDLLRHHEDSWQLGNAGL
                     SLFGNGFAAVAFNPSESQVDNRSKVAFVPDANRIIRYYSTGQSDEKLTKYWSRWVLDP
                     QQFISIFSPHNQDHVAFTLNIPAPATPGLAMEYGDPINYLRMLKELTEPFEHLEEWLS
                     NEGLSGVSVSTNQAAEKMTDRIQRVMHKLHPQSAGNDANIPNLDSKFDASRANTSSAS
                     TISTRMNQPDESDTKHLDVQQKMALFLVATAIGASLAVVLKHWSANKNDIVSYNENQY
                     GE"
BASE COUNT         1392 a         1032 c         1046 g         1560 t
ORIGIN      
        1 ccgatataat ttgttgtttg tttttcaatt tttgataaga gggagggaac tatgactgag
       61 aaaacaacta ttccgccgat gagtgtgagg agcaacaaga gtccatactt gcgaacataa
      121 gaagtgagat accaatagat cttgcgcatg cgtgtattgt agcgatcttt ttgctaaatt
      181 agcaaagagg ctactgataa tatcgtaaat aaaacgatcg aaccaatggt aacgtaaacg
      241 agcacttttt ctaaacctct tttagtgtgg taggtttcgc taccaccacc ccaagctgta
      301 ccaagaccag caccttgtgc ttgaagaagg attgaaacag taataataat cgatagcact
      361 atttgaacga cgagtaacac ctgttgcata gttttcctat tatactttaa ctggcgactt
      421 cttttgagcg agtttctcta atcactgtca ccgtaactgt tcctgggtac gtaacctttt
      481 ctttgatttc atctctgatt tttcggctca atatgaccac gtcatcatcc tgtgattctt
      541 gtggtttgag taatactctc acctcacgtc ctgcttgaat agcatatgct gacttaactg
      601 agttgtaagc agtggcaatt tcttctagct tttgtaagcg agctacgtac tcatcataat
      661 tttcatatct ggcgcctggg cgtgctccag aaattgcatc ggcgatatac accaccattt
      721 gttctggacc agagaaaggt tcatcttcgt gatgttgggc gatacagtca atcactggtt
      781 gtgggatgtt aaacctttta gcaattttca caccaagttc gacgtggctg ccctcagctt
      841 cctctgatac ttttccaata tcgtgtagta aacaaccaag cttgactaca tttacgtcta
      901 acttcagctc gctggctagc ttcataccaa tttttgtttc ttcaagtgtg tgggcaatca
      961 tattttgacc atacgaaaag cgatatttaa atttaccaag caatttcatt agatcaattg
     1021 gcaaattata aataccaaca tcatggcaaa gttttttacc agcttcgaga gtaattttct
     1081 caatttcact tttcaccttc tcaaccgttt cttcaattct tgctggttga attctgccgt
     1141 ctttgattaa gcgctctaag gaaattcgag caatttctct tcggactgga tcgaaacaag
     1201 acaggcggac ttcggtaggt gacatatcca aatcaacatc aacgccggtt tctttttcaa
     1261 aagagttaat gtttctgcca gatttgccaa taattttgcc tttggcttct tcattgggta
     1321 attcaaccac cgaaacggtg tattcagaaa cgtaatcagt tgcgccatgc ttcatggcat
     1381 caacgaggat ttcttgaact ttttcttgag cttcagcttg agcctcttct tgtttttggc
     1441 gtacgaactg ggcaatttct cgtttggaag ttttttctaa ctgagagaaa agctcgcgtt
     1501 tcgcttcatc agaggttaat ccagccacat tctcaatctc ttttatagct tgttgctgta
     1561 attggtctac ttttttctgt gattcagcta ccttttcttg ctgaagattg agattttttt
     1621 ctctactatc aagttgttct aatcgagcgt caattctgtc cagtttgttt tctaatgaac
     1681 gttgttgctc ttcaacttgt cgtgcgacat ctcgggcatc attctcagcc ttacttcgaa
     1741 tggttaatgc ctctgattta gcttcaacga tgatttctct cgcttttgat tgagcctcac
     1801 gcacaacgtc aagagagctt acatcaggaa gtggagtcga aacccttggt tgaattggga
     1861 cgactggagg ctgagtaact ccactggttt ttggtggttg cgcaggcgtt ggtttgacta
     1921 ctgtttttga ttttacgtct gattgaacgc gcttgttttg tttttgattt ttgttgtctg
     1981 tagatgcaaa aagactcgat aaattgctaa aaagtgacat gagtgctcca tttctttaat
     2041 ctgattcata cccaaaggct ttgaacccag atatgctctc acatggaaaa ccagaaaaaa
     2101 actagcaaat agtagaatcc aaaggccccc aatgagctga tggataacta gtagaatata
     2161 aagctagaat catatatttc tggaccagag tagattactg agtacaaaga tcattaatta
     2221 ctatgggtgt attgtaactt gcttacgctt tgctgtcaat cgtagagaaa tgagaaataa
     2281 tattgataaa tcaatattat ttatctcttt cttggcgaat aacatcccaa attttttgct
     2341 taatttcctc aatcgttttt gggtgctctg cgaggtattg tttagcggct tcccttcctt
     2401 gcgccagcac ttcgccctca tatttaaaga agctacccgc tttagtaacg atatttctct
     2461 ccactccaat atcgagcaca tcgccggtaa tcgaaatacc gtaatcatcc atatcaaact
     2521 ccgcgacttg aaatggcggg gccattttgt tcttttttac tctgactctg tgacggctac
     2581 ctacgatttt ctctccttct tgaatgttgc caactttacg aatatctaga cgaacagagg
     2641 cataaaactt gagtgcatta cctccagaag ttgtttctgg attaccaaac attactccaa
     2701 gcttcatgcg aatttgattg gtgaatataa ccaaagtttt agttttattg atggcggcag
     2761 tgagcttacg catggcttga gacattaatc gagcttgggt gcccatctgg tgatcgccca
     2821 tctctccttc aatttctgat tttggtacca acgcagccac tgagtcgata actacaatat
     2881 caactccacc agacctaatg agcgtttctg caatttctaa ggcctgttca ccatagtctg
     2941 gctgagaaat gagtaaatca tctaggttta caccaattaa tttggctcga accggatcta
     3001 aggcgtgttc aacatcaatg aaggccgcgg tgccgccttg tttttgcact tctgcaatgg
     3061 cgtgcaaaca cagcgtggtt ttcccagacg cctctggtcc ataaatttcc acaattctgc
     3121 ctttggggaa tccaccaacg cccaatgcaa gattgagtga aagtgaacca gtggagacca
     3181 ctggcacggt acccagttct tttaattggt ctcccatctt catgattgat cccttaccat
     3241 attctttttc tatctgttgc atggcaataa caactgcttg ggagcgacct gtctcctcgg
     3301 tggtgggggt tactttggct ggctttgtca tagatgcttt ctttcgttat actacgattt
     3361 ggtacgggtt ttattcagac tttactatat tccaacccga aatgtaaatg gtgagtattg
     3421 cgttaagcgt agaagtcttt acaatgagtt gctcattgtg aattcaccag agggaacacc
     3481 tgttccaaaa gaactaccag cggataattt acccagcgtt gaacactctc aaccgctgaa
     3541 agatcgtgag ttttggtggc aatggttaca cgcaggtgaa agacaaatcc gcatcaaacg
     3601 aaccgataaa gagttgctag taggtgaggc gagtgaattt tcagagtcgc tctcaaacca
     3661 agctatggcg aggatattgg cggaactaga gtctgaccgc ctttcaccaa ataaaaacat
     3721 tgtcgatttg ccattcattg aatggcctga agaagttatt actgcactac gatttagaga
     3781 tggcattggg tctttgcttg atagcttgga cgatgctgaa aaaaatgaag taaaatttat
     3841 tgatctttgt gaggtgctaa ttgaaaacga agcaaaaata aaatatggtt ttacagggag
     3901 atattggtcg ccagaaaaac tctcggaagc tgaaattttt gagcgctcta ttgccattgg
     3961 tacctacttt ttagaacaag ttttgataca gcaaaagtgg ggagacgaca tcgatgcagt
     4021 attggtaact tctgcagtat tgccagagac tatatcactc gaaattatca attttgctaa
     4081 acaaaaaggc atactgaaaa aacgaaagcg gattcaggct agagacatca gaaccgcttg
     4141 cagtggagtg tttttcgcag atgtaattgc tgctaaagac aaaaaaattc aaaagaaaaa
     4201 aagagcagtg atactagcac ttgaaccgtt aggtgattta ttacgacacc atgaagattc
     4261 atggcagctt ggaaacgctg gtctctcact ctttggcaat ggttttgctg ctgtggcgtt
     4321 taacccttcg gagtcgcaag tggacaatag atcaaaagtg gcatttgttc cagatgcgaa
     4381 tagaatcata cgctattact caacaggtca atctgacgaa aaacttacta agtattggtc
     4441 aaggtgggtg ttagatcccc aacaatttat ttcaatcttt tctcctcata atcaagatca
     4501 tgtggcgttt acactgaata ttccggcacc agcaactcca ggtttggcaa tggagtatgg
     4561 tgatccaatt aactatctta ggatgctcaa agaattaact gagccgtttg aacatttaga
     4621 ggagtggttg tcaaatgagg gattgagtgg tgtgtcggtt agtaccaatc aagcggcaga
     4681 gaaaatgacg gacagaatac aacgggttat gcacaaatta catccacaat ctgcgggaaa
     4741 tgacgcaaat attccaaact tagactctaa atttgatgca tctcgagcaa acacgagttc
     4801 tgcctcaaca attagtaccc gaatgaatca gcctgatgag tcagatacca agcatcttga
     4861 tgtccaacag aagatggcgc tatttttagt tgccacggct attggtgctt cgcttgcggt
     4921 tgtgttaaaa cattggagtg caaacaagaa cgatatagtg tcgtataatg agaaccaata
     4981 cggggagtag ttcagatggc tagaatgctc gcatggggtg cgagtggtcg
//
LOCUS       DAOQZP010000057         4828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_17877_length_4828_cov_4.453384, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000057 DAOQZP010000000
VERSION     DAOQZP010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17877_length_4828_cov_4.453384"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2003
                     /locus_tag="PKH60_04270"
     CDS             <1..2003
                     /locus_tag="PKH60_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNQ17287.1"
                     /translation="YYQPEAYIPSTDTYIEKEATINEEIDRLRLATTTNLMTRRDAIV
                     VASVSCIYNLGSPVEYGRYLLRIAEGEIIQKRTLLLQLENLQYDQTETDLFRGMYRLR
                     GDSIQVWPAYEDKAMRIDILENQIEHIAWIDPVSGKPLPITEQQKPDKYEYVLYPAKH
                     YVIDPVSQKTAVEQIRIDLKDQILKLEAQGKVVEPFRLRQKVEYDLEMIEQFGFVNGI
                     ENYSRYFDNRKPGDPPFSLLEYFAENARQFNTNGFLTIVDESHMTIPQITGMHRGDEA
                     RKKTLIEYGFRLPSALDNRPLRFDEFDARVPLMVAVSATPASTEIKAAGGKLVEQLIR
                     PTGLLDPTIELRDAENQIEDLVIEILKRKMKGQRVLVTTLTKKMAEALTEYLNTEEKV
                     RALWQSARAKQQANNDLIESNQVDSESVVWRGEQLPIEQIEIGPINPIYFAHLQHQPK
                     STTEKVVLVELNEMPKVAYLHSDIETLDRSDILRELREGKYDVLVGINLLREGLDLPE
                     VSLVAIIDADKEGFLRSETALVQTMGRAARHLEGHTILYAKNLTGSMQRAIKETQRRR
                     QVQQQYNQTHGITPESINKPIRTDMIVNRKKKEENQTKHSVSIYLTERERLDLNEIHP
                     ENYTPEEKKKLIKQLKKQMSAAAKDLDFELAAKLRDAVAILL"
     gene            complement(2012..2746)
                     /locus_tag="PKH60_04275"
     CDS             complement(2012..2746)
                     /locus_tag="PKH60_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNQ17288.1"
                     /translation="MSLFQQIVSDLQSRANPEKKLVLQRFFKTAPGQYGEGDVFLGVI
                     VPDQRIVAKKYYQVADFGVVERLLASPIHEYRLTALIVLTYQFPKVELEKKQALFDFY
                     LGQTAKINNWDLVDLSAPNIVGEYMLATKLDMERLLQLEQSPLLWERRISIISTLAFI
                     RAVKKGRVGSEWLQPPLILAEKLLADKHDLMHKAVGWVLREVGKADERVLREFLEKYS
                     SVMPRTALRYAIERLDEESRQKYSKR"
     gene            complement(2894..3457)
                     /gene="efp"
                     /locus_tag="PKH60_04280"
     CDS             complement(2894..3457)
                     /gene="efp"
                     /locus_tag="PKH60_04280"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNQ17289.1"
                     /translation="MPSIKAGNIRKGTHIRFKNQPYLVTKADFTSPGKGSAFMRCRLK
                     SLQTTSTVEFTFKSNETVEELDISSVKMQYLYHDADEVVFMNQRNYEQASVPIGLLED
                     QLQLLLPEMEAYVLLFEDKPIGVSLPTKITMTVVRADDAVAGDTVGQAKKEAEVETGL
                     LVQVPLFIKKGEKIIVDTATKQYVSRA"
     gene            3557..>4828
                     /locus_tag="PKH60_04285"
     CDS             3557..>4828
                     /locus_tag="PKH60_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNQ17290.1"
                     /translation="MNQAFKNFATIFVLSISLFFLSNTKVLANAFNFNSSTGPDTFTC
                     YASDLGCEQVNHVYFINATGDFLWQTTAWTSSPNHMLEYYSWTKDFTWEQDTNPSQQP
                     IESHLGVILPVRMKNNGIPGTYQGTLYVDAKTCNRAVTPWDCYFRGAASYTMTMTVLA
                     DPTATPRPTTPPPTATPTTRPPSATATPTGSQPTLTPTPTTPSGQATPTPISGVTSPT
                     PTSSTSQTTANTTTNSGTADSTGTQTKPKTCDVTAPNAPKLLSVKPVSQTSVTLEWSA
                     VSEATHYSVSYGTKSGTYLYGVSNTGNTTSFTVKSLGKNSTYYFVVQAISDCAPSKIS
                     NELSTLVDQTLPEKSFEELVLAMESSESAGITDQEPDSTAVHNQKVTQVYPLNQLLAL
                     VVGGFVAMVIGLITFIKKSPTNPISRFISKMI"
BASE COUNT         1494 a         1107 c          929 g         1298 t
ORIGIN      
        1 attactacca acccgaagcc tacatcccca gcaccgatac ctacatagaa aaagaagcaa
       61 caatcaacga agaaatcgat cgacttcgtt tggccaccac cacaaactta atgacgcgaa
      121 gagatgccat cgttgtagct tcagtctcat gtatttataa cttgggttct ccagtcgaat
      181 acggcagata ccttctgagg attgctgagg gtgaaattat tcaaaagaga accctgttgc
      241 tgcaattgga aaatttacaa tacgatcaga ctgaaaccga tttgtttcgg ggaatgtatc
      301 ggttacgagg tgattccatt caggtctggc cagcctacga agacaaggcc atgcggatcg
      361 atattttgga aaaccaaatt gaacatatag cctggataga cccagtttcg ggtaaaccac
      421 tacctattac agaacaacaa aaaccagata aatatgagta tgttttatac ccagccaaac
      481 attacgtcat cgatccagtt tcacagaaaa ccgcggttga acaaatcaga attgatttaa
      541 aagaccaaat ccttaaactt gaagcccagg gcaaggtagt tgagcccttc agactcagac
      601 aaaaagttga atatgactta gagatgatcg agcaatttgg gttcgtgaac gggattgaaa
      661 attattcccg ctattttgat aatcgtaagc caggcgatcc accattttca ttacttgagt
      721 actttgcaga aaatgccaga caatttaaca ccaatggttt tctcactatc gttgatgaaa
      781 gtcatatgac aattccacaa attactggca tgcatcgcgg tgatgaagcg aggaaaaaaa
      841 ctttaattga atatggtttt cggttaccaa gtgcgctaga taaccgaccg ctcaggtttg
      901 atgaatttga cgcacgggta ccgctcatgg tggcggtttc agctacacct gcaagtacag
      961 aaatcaaggc ggctggggga aaattagttg aacaattgat tcgacccact ggattgttgg
     1021 atcccaccat tgagcttcgt gatgcagaaa atcagattga ggatttagtg attgaaattc
     1081 ttaaacgcaa aatgaaaggt cagcgggttt tggtcacgac attaacaaaa aaaatggctg
     1141 aggcgttgac tgaatactta aacacggaag agaaagtgcg cgcgctctgg cagagcgcac
     1201 gcgccaaaca acaagcaaac aatgatttga tagagtccaa tcaagtagac tcagaatccg
     1261 tggtatggcg aggtgaacaa ttaccaatcg aacaaattga gattggaccc attaatccta
     1321 tctattttgc ccacctccaa catcaaccaa agtctacgac tgagaaggtc gtcttggttg
     1381 agctgaatga aatgccaaag gtggcctatc tacattctga cattgaaacg cttgatcgaa
     1441 gtgatatttt acgagaactg cgggaaggta agtatgatgt gttggtcggc atcaatttgc
     1501 tcagagaggg tcttgatctg ccagaagtca gtctggtggc catcattgat gcggataaag
     1561 agggtttttt acgatcggag actgcgcttg ttcaaaccat ggggcgcgct gcccgtcatc
     1621 tagaaggaca cacaattctc tacgccaaaa atctaaccgg ttcaatgcag cgcgccatca
     1681 aagaaaccca acgtcgccga caggttcagc aacaatacaa ccaaacccac gggatcactc
     1741 cagaatcgat caacaaacca attagaaccg acatgattgt taatcgtaag aaaaaggaag
     1801 aaaaccaaac aaaacatagc gtatcaatct atttgacgga acgtgagcga ctcgatctca
     1861 atgaaataca cccagaaaac tacacgccag aggaaaaaaa gaagctcata aagcaactaa
     1921 agaaacagat gagtgcggct gcaaaagatc ttgatttcga attggccgca aaattacgcg
     1981 atgcagtggc aattctcctc tgataagtga gtcatctctt tgagtatttt tgtctcgatt
     2041 cttcatccaa tcgctcaatt gcgtagcgta gcgcagttcg gggcatcaca cttgaatact
     2101 tttctaaaaa ttctcgtaat acgcgctcgt cggctttgcc gacttcgcgc aatacccatc
     2161 ccactgcttt gtgcatcaag tcatgcttat ctgcaagcag tttttccgcc agtatcaaag
     2221 gaggttgcag ccattcgcta ccaactcggc ctttctttac cgctctgata aaagccaaag
     2281 tagaaataat actgattcta cgttcccaga gtaggggaga ctgctccaac tgaagcagtc
     2341 gctccatatc cagttttgtc gccaacatgt actcaccaac tatatttggt gctgataagt
     2401 caaccaaatc ccagttgttt atctttgcag tttgacccaa ataaaaatcg aataatgctt
     2461 gttttttttc taattctacc ttcgggaatt ggtaggtcaa cacaattaat gctgttaacc
     2521 gatattcgtg gattggacta gctaaaagtc tttccaccac accaaaatct gccacttgat
     2581 agtacttttt ggctactatc ctctgatctg gcactatcac acccaaaaaa acatctcctt
     2641 caccatattg accaggagcg gtcttaaaaa aacgttgaag tacgagtttt ttctctggat
     2701 tagctcgtga ttgtaaatct gaaacaattt gctgaaaaag cgacattaat tgattctaca
     2761 acagagctat tattatcgtc attgacttac ttatactatt tgttataatg acgatacctc
     2821 agaaggttct tcctcaccgg gagctacgtt ctgagactaa aaaatctcgc cccaagcggg
     2881 attttttagt aacttacgcc ctagaaacgt attgctttgt tgcagtatca acgatgattt
     2941 tttcaccttt tttgatgaat aggggcacct gaaccaataa acctgtttct acttcagctt
     3001 cttttttggc ttgaccaacg gtatcacctg ccaccgcatc atcagcccgt accaccgtca
     3061 tggtaatttt cgttggcaga ctcactccta ttggtttgtc ctcaaatagc aaaacatagg
     3121 cttccatttc tggcagtagt agttgtagtt gatcctccag caaaccaatt gggacgctgg
     3181 cttgctcata gttccgttga ttcatgaaca ctacttcatc agcatcatga tacaaatact
     3241 gcattttgac actcgaaata tctaattctt cgactgtttc attagactta aaagtaaatt
     3301 caactgtgct cgtcgtttgt aagctcttca gccggcagcg cataaacgcc gagcccttac
     3361 ctgggctagt aaagtctgct ttcgtgacta aatacggctg attcttaaac cgaatatggg
     3421 tacctttgcg tatgtttcca gctttaatac ttggcataag tggatattgt acactaagta
     3481 ttatagatac acataccatg cttttagacc tataatttga ctaatgtata ttatatatgt
     3541 ataataaatc atagatatga atcaagcttt taaaaacttc gccactatat tcgtcctgag
     3601 tatcagtttg ttttttttaa gtaatacaaa ggtgctcgca aacgctttca attttaattc
     3661 ttcgaccgga cccgatacct ttacctgtta tgccagtgat ctaggttgcg agcaagtcaa
     3721 tcatgtgtac tttattaacg ctacaggaga ttttctgtgg caaactaccg cgtggacatc
     3781 gagtcccaat catatgcttg aatactatag ttggacgaag gattttacat gggaacaaga
     3841 tactaatccg agtcaacagc cgattgaatc acatcttgga gtaattttgc cagtacgaat
     3901 gaaaaataat ggaattcccg gcacatatca aggtacgctg tatgttgacg caaaaacctg
     3961 taatcgggct gttactccgt gggactgtta ctttcgtggc gctgcatcgt atacaatgac
     4021 catgacagtt cttgccgatc caacagctac ccccagacca accacaccac caccaacagc
     4081 tactccaaca actcgaccac caagtgcaac tgccacacca acgggttcgc aacctacact
     4141 cacacctaca ccaacaactc cctctggcca agcaacacca accccaatca gcggagtgac
     4201 ttcaccaaca cccacaagtt caacgtcaca aacaactgca aataccacaa ctaattcagg
     4261 tactgcagat tcaaccggaa ctcaaacaaa gccaaagact tgtgatgtca ctgctccaaa
     4321 tgcacccaaa ttactttcag taaaaccagt ctcccaaact tcagttactt tggaatggtc
     4381 cgccgtctct gaagcaacac attactctgt atcttatggc acgaagtcag gtacctatct
     4441 gtatggagta tcaaacactg gtaatacaac ttcattcaca gtaaaaagtc tgggtaaaaa
     4501 tagcacatat tactttgttg ttcaagccat ttctgactgc gccccttcaa aaatttctaa
     4561 cgagctcagc acattagttg atcaaacact gccagagaag tcatttgaag aattagtgtt
     4621 ggcaatggaa agttcagaat cagctggcat cacggaccaa gaacccgatt ctacagcagt
     4681 tcataatcaa aaagtcactc aggtctatcc actcaaccaa ctacttgcgc tcgtcgtggg
     4741 aggatttgtt gcaatggtaa ttggactaat cactttcata aaaaaatctc caacgaatcc
     4801 aattagtaga tttatttcaa aaatgatt
//
LOCUS       DAOQZP010000058         4806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_17981_length_4806_cov_5.114292, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000058 DAOQZP010000000
VERSION     DAOQZP010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17981_length_4806_cov_5.114292"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PKH60_04290"
     CDS             complement(<1..731)
                     /locus_tag="PKH60_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17291.1"
                     /translation="MSNQDDLINNAIEDYIQGEQQKYSQWKQSSREYFKDRSKHKGSE
                     SWSSNSNRLPTSEEIASQRGWPSESDETKLKRVFFTSFRSAVEKKLGEENLAKVDEVL
                     GLLYGVYLVNYITGGLLINKYFDYYSFQKWDMNQWQFQTANLDPNNLDDWPVVFNMTN
                     SRSSSNYNNLFGISIQIEVGVARYVGHKPYEQQIVSFGEREIIRKSFLGLGRKKVKTP
                     VIQFSISAVDEAVLTGIEEAHHSLLT"
     gene            844..2247
                     /locus_tag="PKH60_04295"
     CDS             844..2247
                     /locus_tag="PKH60_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17292.1"
                     /translation="MWGKRKKLRVIFQSMLFIGLLLVILAGVGQLGIVREFFGQASFI
                     PANLFVNTKAVLGPLPRPWRNLAQGGESHAWRIAPIANSVLALHPEYIRLDHIYDFYD
                     IVSGSPGNFTVDFSKLDLVLNDIKSVGAVPYISLSYMPPALGNGDITGAPVNYSDWQS
                     LVQQTIQHISGTLGFRDVYYEVWNEPDLFGGWKYYGNKSYLDLYAASARGAAATRNTQ
                     AFKIGGPATTALYKNWIDALLTFTSKNNLRIDFISWHKYTYDLDQYRVDLAQARTWVS
                     AYPQYNGQMEYQITEWGHNSELDAGYDGRLGAAHTVASAITMVGALERGFVFEIQDGL
                     DPSGKPYWGRWGLLTANEHGAKPKPRYWALRMLDSLASQRLQLLGQGSKVKALSAKTE
                     DGKVQTILANFDPKGIGQETVPITYQFITPGNYELSIKYLSGDTRIDKIATTTATLRM
                     LVPVPSNDVVFTELVKK"
     gene            complement(2225..3334)
                     /locus_tag="PKH60_04300"
     CDS             complement(2225..3334)
                     /locus_tag="PKH60_04300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNQ17293.1"
                     /translation="MGKKLRVALVHDYLAEYGGAERVLEALHQLFPDAPVYTAFVDQE
                     RLGIQWQRFSDWDIRTSWLTKIPFHKRLFSPLRIFAPQFFSGFDLSEFDLVISSTNAY
                     FAKAVKIRHGAVHLSYCHTPSRSLWGYTTMTNWKKHAIIRIGGELINHYLRVVDVQIA
                     RERVTHFIANSKETARRIEKFYRLPATVVYPPINVPAKLSTAKKQDYFLYINRLAWSK
                     HPEIAVMACSKLGLPLKVVGSGKMMPQLRQIAGPTVEFLGFAKDADLQALYSNAQALL
                     YPVEDEDFGMVPVEAMAQGTPVIAHASGGPLETVIDGKTGVLFSDLTIEGLTKALQRF
                     RKEKFSPDVIWKHSKQFDQAHFNAGIQQVISSQVQ"
     gene            3383..3715
                     /gene="rnpA"
                     /locus_tag="PKH60_04305"
     CDS             3383..3715
                     /gene="rnpA"
                     /locus_tag="PKH60_04305"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNQ17294.1"
                     /translation="MLPKEHRYSSRNEIRQVFRNATRKHSNSFMWLSSQANNRANLPW
                     RAAIIVSKKVAPLATDRNAIRRKISEALWQQKDQLKQGFDIVILAQKRSLISTAASLY
                     QEVQLMVS"
     gene            complement(3712..4704)
                     /locus_tag="PKH60_04310"
     CDS             complement(3712..4704)
                     /locus_tag="PKH60_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HNQ17295.1"
                     /translation="MSSQKTPVEKLAFDRENVRDRLILVDGHALIYRAYHAFPELTDH
                     TGMLVNAVYGFTRILLTTIRDFHPEYLAVCFDSKEKTKRAEEFEAYKANRPEMPDDLK
                     PQIEIIKSVVTALNIPQFAHPGLEADDLIGSFSTQLYEKNPDEQPLTIVLTGDKDLLQ
                     LVDDNVHVFIPARSKTRGDIEYDAQTVQRQIGLRPDQIVDMKALMGDASDNIPGVKGI
                     GPKTAVALLQLRQTLDGVYASAEEIEKSGAYVAPFTKSVVAKLVVDKTQAYQSQKLAT
                     IQRDHHLDFKLETCKVSSYNKEEAVKILQQYDFRSLISLLPRDSFEVGVQAALF"
BASE COUNT         1351 a         1071 c          975 g         1409 t
ORIGIN      
        1 gttaacaaag agtgatgtgc ttcttcaatt cccgtcaata cagcttcatc caccgcagat
       61 attgagaact gaatcacagg agtttttacc tttttacgac ctaatcctag aaatgatttt
      121 ctaataattt cccgctctcc aaatgacact atttgttgtt cgtacggttt gtgaccaaca
      181 tatcttgcaa caccaacttc tatctgaatt gaaattccaa acaaattgtt gtaattcgag
      241 gacgatctgc tattagtcat gttgaataca actggccagt catcaaggtt atttggatct
      301 aagtttgctg tttgaaattg ccactgattc atgtcccatt tttgaaagct gtagtaatca
      361 aagtatttgt tgattagtaa tcctccagta atataattga ccaaatatac gccatacaac
      421 aaacctagta cctcatctac ctttgcaagg ttttcttccc ctaatttttt ttctaccgca
      481 gatctaaacg aggtaaaaaa aactctcttt aattttgtct catcgctctc actcggccaa
      541 cctctttgtg atgctatttc ttcggatgtt ggtaatcgat tgctatttga actccaagat
      601 tccgagcctt tatgtttgga cctatcttta aaatattctc gtgatgattg cttccattgg
      661 ctatattttt gttgctctcc ttgaatataa tcttcaattg cattgttgat aagatcgtct
      721 tgattactca taatggtact tataatagga ttctgactga atgatatcta gtagcaatca
      781 aacataatac gcgttttctc ctgacgcttg ttgatcgagc atattttcga gtatagtgga
      841 attatgtggg gaaaacgtaa aaaactacgc gtgatttttc agtcaatgtt gtttattgga
      901 ctcttactcg taattctggc tggcgttggt cagctcggga ttgtgcgtga attttttggt
      961 caagcgagtt ttataccagc aaaccttttc gttaatacaa aagcagtctt gggtcctctc
     1021 ccccgaccct ggcgtaattt agctcaaggt ggtgaaagtc atgcttggag aattgcaccc
     1081 attgcaaatt cagttctagc actgcatcct gaatacattc gactggacca tatctacgac
     1141 ttttatgata ttgttagcgg ttcccctggt aacttcacag ttgatttttc caagttggac
     1201 ctcgtcttga acgacataaa atcagtaggg gctgtgccat atatttcact ttcgtatatg
     1261 ccacccgctc taggcaatgg tgatattaca ggtgctccag ttaattatag cgactggcaa
     1321 tcactggtac agcaaactat ccaacatatc agcggaactc ttggcttccg cgatgtgtat
     1381 tatgaagtct ggaatgaacc tgatttattt ggtggatgga aatactatgg caataaaagt
     1441 tatcttgatt tatatgccgc ttccgctcgt ggagcagccg ccacaagaaa tacacaagca
     1501 tttaaaattg gcggacccgc gactaccgca ctctataaaa actggataga cgcgttactg
     1561 acttttacca gcaaaaataa cttacgcata gatttcattt cttggcataa atacacctac
     1621 gatttagatc aatatcgggt ggatttagct caagccagaa cttgggtttc ggcatatcct
     1681 caatataacg gacaaatgga ataccaaatt actgaatggg gacacaatag tgagctggat
     1741 gcagggtatg atggcagatt gggcgctgcc cacaccgtgg ccagcgccat taccatggtc
     1801 ggagctcttg agcgaggttt tgtttttgaa attcaggacg gtctcgaccc aagtggcaaa
     1861 ccatattggg gtcgctgggg cttactcaca gccaatgaac atggcgcaaa accaaaacct
     1921 cgctactggg cactcaggat gctcgatagt ctagcctccc aacgcttgca gttgctcggc
     1981 caaggttcaa aggtcaaagc cctttcagca aaaacagaag atggtaaagt acaaactatt
     2041 cttgccaatt ttgatcccaa aggtattggg caagaaaccg tgccaattac ctatcaattt
     2101 atcaccccag gcaactacga actttcaata aaatatctgt ccggcgacac tagaattgat
     2161 aaaattgcta ccaccactgc aacactacgg atgcttgtcc cagtaccatc aaacgacgtc
     2221 gtatttactg aacttgtgaa gaaatgactt gctggattcc cgcgttaaag tgagcttgat
     2281 caaattgttt ggaatgtttc caaattacat caggactaaa tttttctttt ctgaatcttt
     2341 gtagcgcttt cgttaatcct tcaatagtga ggtcggaaaa taacacccca gtcttaccat
     2401 ctatgaccgt ctctagcgga cctccagaag catgagctat cacaggagtt ccctgtgcca
     2461 ttgcttccac cggcaccata ccgaaatctt catcctctac cggatacagc agcgcttgag
     2521 cgttgctgta taaggcttgc aagtcagcgt ctttagcgaa acctaaaaat tctacggtcg
     2581 gtccagcaat ttggcgtaat tgtggcatca ttttgcccga tcccactacc tttaacggta
     2641 aaccaagttt tgaacaagcc atgacagcaa tttctgggtg tttagaccat gctaggcgat
     2701 ttatgtagag aaaataatct tgctttttcg ctgtagataa ttttgcaggc acattgatgg
     2761 gtggataaac taccgtggct ggcaaacgat aaaatttctc aattctgcgt gcggtttctt
     2821 tggaattggc aataaaatgg gtaactcgct cccgtgcaat ttgcacatct accactcgca
     2881 agtagtgatt gatcaattca cccccaatcc taataatggc gtgttttttc cagttagtca
     2941 tggtggtata gccccagagt gaacgactgg gggtatgaca atagcttaga tgaaccgcgc
     3001 cgtgacgaat tttgactgct ttggcaaaat aagcattggt agaagaaatc accaagtcaa
     3061 actcagatag atcaaaacct gaaaaaaatt ggggtgcaaa gatgcgcaga ggcgaaaaca
     3121 gtcgtttatg gaacggaatt tttgttaacc agctggtgcg aatatcccaa tcggaaaagc
     3181 gctgccactg tataccaagt cgttcctgat caacaaacgc cgtatatact ggcgcatctg
     3241 gaaacagctg gtgcagcgct tccaacaccc gctccgcccc accgtattca gctaagtaat
     3301 catgtaccaa cgcgactcgt aatttttttc ccatgtagga gtattctacc tcagttccta
     3361 tatcggttgc tataatacaa caatgctccc caaagaacat cggtactcct cacgaaacga
     3421 aatccgccaa gtttttcgca atgcaactcg caaacacagc aattcgttta tgtggctgag
     3481 tagtcaagca aataaccgcg ccaatctacc ctggcgagca gcaatcattg tcagcaagaa
     3541 agtggcgcca cttgccaccg atcgaaatgc aattagaaga aaaattagtg aggcgctgtg
     3601 gcaacaaaaa gatcaactta aacaaggctt cgatattgta attcttgcac aaaaacgatc
     3661 tctaatttca acagcagcaa gtctttatca agaagttcag ctgatggtga gttaaaatag
     3721 cgccgcttgc acgccgactt caaaggaatc tcttggaagt aaactgatta atgatctgaa
     3781 atcgtattgc tgtaaaattt tgactgcttc ttctttgttg taacttgaaa ctttgcaggt
     3841 ttcgagcttg aaatctaagt gatggtcgcg ctggatcgta gccagttttt gactttggta
     3901 tgcctgagtt ttatcaacta ctagcttcgc gaccaccgat ttggtaaacg gtgccacata
     3961 tgcacctgat ttttcgattt cttctgcaga tgcatacact ccgtcaagcg tttggcgcaa
     4021 ctgcaagagc gcgacagctg tttttggacc aatccccttt actcccggaa tattatcaga
     4081 agcatcgccc atcagggctt tcatatccac aatttgatca ggtcgtaacc caatttgccg
     4141 ttggactgtc tgcgcatcat attcaatatc tccccgggtt ttactccgag caggaataaa
     4201 aacgtgtaca ttgtcgtcaa ctaactgcaa aaggtcttta tcaccagtta atacaatagt
     4261 taaaggctgt tcatcaggat ttttttcgta gagctgagta gaaaatgaac caattaaatc
     4321 atcggcttct aatcccgggt gagcaaattg cgggatattg agcgcagtaa caaccgattt
     4381 gatgatttca atttgtggtt ttaaatcgtc tggcatttcc ggccgattag ccttgtatgc
     4441 ttcaaactct tctgcgcgct tcgttttttc tttgctgtca aaacagactg ctaaatattc
     4501 tgggtgaaag tcgcgaatcg tcgtcagtaa gatcctggtg aaaccataca cggcattgac
     4561 caacatacca gtatgatcag ttaattcggg aaaggcgtgg taggcgcggt agatcaatgc
     4621 atgaccgtca acaagtatta accgatcgcg cacgttttct ctatcaaatg cgagtttttc
     4681 tactggagtt ttctgcgaag acataacaat atcctactcc atcataattc atctacaaag
     4741 cgtaggttcg caaacgaagt gcaccaccct gacggtataa tcgtcagctg aagggaggtg
     4801 tacgat
//
LOCUS       DAOQZP010000059         4746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_18273_length_4746_cov_4.404818, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000059 DAOQZP010000000
VERSION     DAOQZP010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18273_length_4746_cov_4.404818"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..792
                     /locus_tag="PKH60_04315"
     CDS             136..792
                     /locus_tag="PKH60_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17296.1"
                     /translation="MHYPKPSTIKPKDIEYMIDEINALNKEAHQYLRMPHLILNLTFA
                     ALPLIIAQSWGGKPTDLSLLVAPLIFGILTMYNLNLAAEAAGLSEIRDRLGNQVNKAI
                     KQPIFLMRVLSDARRGSPGTVAAYGLAALIILITMTYGLIHAKQVNQWWFLLQITVTF
                     IPILATGIGVIELAQTRIKINDELDSIFGQSSRPTSAPSHADSWWKRILTALTKRVSN
                     "
     gene            892..1275
                     /locus_tag="PKH60_04320"
     CDS             892..1275
                     /locus_tag="PKH60_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17297.1"
                     /translation="MTMTLLEHHVEILKSKQFMEELTGNVGDRTRKPEVIAQEIVDNL
                     KKVDPYSVMLSDPERYQAFLSIFAEAVEAFRNGQIDEVGDFQVKRDVICDACGLVSCW
                     RKNDTLSLEEAIEAIRNSQKVLLFT"
     gene            1325..1906
                     /locus_tag="PKH60_04325"
     CDS             1325..1906
                     /locus_tag="PKH60_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNQ17298.1"
                     /translation="MLQQPIEDTHEKILATTTHLVAVYGCAGVSMRLVGKHVPIAQSV
                     IYHYYADKDSLLKAMYDRANRMLGQNRQALPQPKSATKRLENIIRFQIEHAELVVAVL
                     KYYLQYREDFAKQESGTLPEKAILHVEEVLEYGHQTGEWVITDLPTQSKILAHAINGY
                     LLEYYPHMPTGKSARTLVNALVEFTKSALTPRS"
     gene            2088..2360
                     /locus_tag="PKH60_04330"
     CDS             2088..2360
                     /locus_tag="PKH60_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17299.1"
                     /translation="MNKTTLTLGAAAVAVVIGAGALVNNAVFAKSPASATKPTTVAEK
                     TLPPTAKGEMKPAESNIVEIVLKEDGEFDVLQAAVVKAGLVDALSG"
     gene            2367..2672
                     /locus_tag="PKH60_04335"
     CDS             2367..2672
                     /locus_tag="PKH60_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNQ17300.1"
                     /translation="MTVFAPTDAAFIKTLAVADEAAAIAAVEGLPVADLSKILLYHVT
                     EGRRNSQSVLAAPQYPMLNDMMLSQTELTTAGIAETDISASNGIIHVINSVLMPAAK"
     gene            complement(2771..4195)
                     /locus_tag="PKH60_04340"
     CDS             complement(2771..4195)
                     /locus_tag="PKH60_04340"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HNQ17301.1"
                     /translation="MKVSHFWTFAIICCAVIFFVGSSAISLHRFWQYDAQYYDFGIFD
                     TALWKVAHFQPPIIDHFSKAGGKLIFADHFSPAIFLLAPLYWLFPWSETILVAQAAIV
                     TLSGLVLVLIVKRVLKSSLASFCVLATYFLYAGLQNALWWDFHEVTIATLPIALLFYF
                     YISKNKRLHIALFIFCLLFKETLFAFGIGWAVFVFFNRKEWRSTAILFGVVSIAWYFA
                     TTQILIPYFRGEAYFYQINQTALKTTSDWLAYIFMPFEKLKYSFQSLYSFGLTAVFVP
                     ELSVVFLLHYLLRFLSDSPQRHTLGLHYNAEIAPVYAFAMILALQRIGVFCKKNCGVK
                     RAHLWIALVIVFNALLLYQFVTKGPFRLSYIPDFYKNTQNFVYLDRLRNQIPINATIA
                     AQSNLASRLTHQKQLWILWENYEQHQADYIFFDSRDGQNPNNYAGIKDITQMRDRIAS
                     DPNYELVAKEDQAYLYKRTEFINE"
     gene            complement(4259..4540)
                     /locus_tag="PKH60_04345"
     CDS             complement(4259..4540)
                     /locus_tag="PKH60_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17302.1"
                     /translation="MTWETSKTGRFGSSELKMLEEMPIEELIQAVLSSQPLVALRVFG
                     ELVLQRAQDRYLALVEDPNFKDTITGFEADADVSKADFIYEQVYLILAN"
BASE COUNT         1507 a         1017 c          914 g         1308 t
ORIGIN      
        1 acctgtaccg gttttccggt ccgtgggttc aaatcccacc gtctccgcca cttttgagtt
       61 ccattctacc cccataatca caggtcagaa attatgagat aattatcagt agttaaggcc
      121 tactgaatta tcattatgca ctaccctaaa ccctcaacaa ttaagcctaa agacattgaa
      181 tatatgattg atgaaattaa cgctcttaat aaagaggctc atcaatatct ccgcatgccc
      241 catctcatac taaatctgac atttgcagct ctaccactta ttattgcaca atcatggggt
      301 ggaaaaccta ctgatctcag cctacttgtt gcacctttga tttttggcat tcttactatg
      361 tataatctca acctagccgc tgaagcagcc ggcctatctg aaatacgaga cagattggga
      421 aatcaagtca acaaggcaat aaaacaacca atttttctca tgcgagtact ttccgatgcc
      481 cgcagaggtt caccgggcac agttgcggcc tacggcttgg cagctctgat catactcata
      541 acgatgacct acggacttat ccatgcaaaa caagtaaacc aatggtggtt tttgttacaa
      601 attacagtta ctttcattcc cattctcgca acgggcattg gagtcatcga acttgcccaa
      661 acgcgtataa aaatcaatga tgaattggac agtatatttg gacaatcatc aagacctact
      721 tccgcaccct ctcatgccga tagctggtgg aaacgcattt tgacagctct cacaaaacgc
      781 gtttcgaatt aacactacat atatgtaatc aacccccact tttgttatct aggataagaa
      841 gtccaaattt cgtcccgcgc tccccacatt gctgctataa tcatgaatta gatgacaatg
      901 actttgcttg agcatcatgt tgaaatactc aaatcaaaac aattcatgga agaattgacg
      961 ggcaatgtag gagacagaac tagaaagcca gaagtaattg ctcaagaaat agttgataac
     1021 ttaaagaaag tggatcctta ctcagtaatg ttaagtgatc ccgaaagata tcaggcattt
     1081 ttatcaatat ttgctgaggc tgttgaggct tttagaaatg gccaaattga tgaggtaggt
     1141 gattttcaag ttaaacgcga tgtcatctgt gatgcgtgcg gacttgtttc ttgttggagg
     1201 aaaaacgaca ccctatctct tgaagaagcc atcgaagcaa tccgaaacag tcaaaaggtc
     1261 ttgctcttca cataagtatt tagaactagc gttctatatg ttttgtggta taattcgctc
     1321 agttatgttg caacaaccta ttgaagacac acatgaaaaa attttagcta cgacaactca
     1381 tttagttgca gtctatggct gcgctggagt gagtatgcgg ttggttggca aacatgtgcc
     1441 aattgctcaa tcagttatct accactacta cgcagacaaa gattcactgc tcaaagccat
     1501 gtatgaccgt gccaatcgta tgctgggtca aaatagacaa gcactaccac aacccaaatc
     1561 cgcaacgaaa cgattggaaa atataattcg gtttcaaatt gagcatgctg agctagttgt
     1621 agctgtactc aagtactacc tccagtatcg agaagatttt gccaaacaag aatctggcac
     1681 attaccagaa aaagccattc ttcacgtcga agaagtcttg gaatatggtc atcagacagg
     1741 tgaatgggtc atcactgatt taccaactca atccaaaatc ttggcccatg ctattaatgg
     1801 ttatctgctc gaatattatc ctcacatgcc aaccggcaaa tctgcccgaa ctttggtcaa
     1861 cgcgcttgtt gagtttacca aaagcgcact gacgcctcgt tcttaaacct ttttgtatct
     1921 cttctttttc attgtaaaaa attggtttta actactattc ttgagttgaa ttgggtgttg
     1981 acaatgtaga acgatcgttc tatattatcg aacagttgtt ctattctaaa tgtataatta
     2041 agaacaattc acagtagtaa agtagtaata aattaaagga ggttctcatg aacaaaacaa
     2101 ctctaaccct tggtgctgca gcagtagctg tagtcattgg cgctggcgca ctggtaaata
     2161 acgccgtatt tgccaaaagc ccagcctcgg caactaaacc cacgacagtg gcggaaaaaa
     2221 cgcttccccc aactgctaaa ggtgaaatga aaccagcaga atccaacatt gttgaaattg
     2281 tgttaaagga ggatggtgag ttcgatgtgc tacaagcagc agtagtcaaa gctgggcttg
     2341 tagatgcttt gagtggctaa ggtcaaatga ctgtatttgc tccaactgat gcagctttta
     2401 tcaagacact agctgtggct gatgaagcgg ctgccattgc tgcagttgaa ggtttgccag
     2461 ttgcagatct tagtaagatc ctactctacc atgttactga aggtaggcgt aatagccagt
     2521 cagtattagc agcacctcag tatccaatgt taaacgacat gatgctgagc caaactgagc
     2581 tgaccaccgc tgggattgct gaaacagata tatctgcaag caatggcatc attcacgtca
     2641 tcaacagcgt gctaatgcca gcggcaaaat aattcttgag cacttctcac tgggtggacc
     2701 acaaagagca gccccgccat tcggcggggc tgcgtgctat caagcactac atcccaacta
     2761 ataattaaac ttactcattg ataaattcag ttcttttata caaatatgcc tgatcctctt
     2821 ttgcaactaa ctcatagttt ggatcactcg caattctgtc cctcatttga gtgatatctt
     2881 tgatacctgc ataattgttg gggttttgac catcacgact atcaaaaaag atatagtctg
     2941 cttgatgctg ttcataattt tcccaaagaa tccaaagttg tttttggtgt gtaaggcgag
     3001 aagcaaggtt gctctgagct gcaatagttg cattgattgg aatctggttt ctcagcctgt
     3061 ctaaatacac aaaattttgc gtgtttttgt agaaatctgg tatatacgac aatcggaaag
     3121 ggccttttgt gacaaactga tagagcagca aagcattaaa aacaattacc aacgcaatcc
     3181 acaggtgcgc tcgttttaca ccacaattct ttttacaaaa tacaccaatc ctttgaaggg
     3241 caagaatcat cgcaaaagca tacactggtg ctatttccgc attgtaatgc agacccaacg
     3301 tgtgtctttg tggcgaatca gaaagaaatc tgagtaagta atgcaaaaga aacacgacgg
     3361 aaagttctgg cacaaacaca gcagttaaac caaaactata caaactttga aaggaatact
     3421 ttaatttttc aaatggcatg aaaatatatg ccaaccaatc cgatgttgtt ttaagtgctg
     3481 tttgattaat ttgataaaag taggcctctc cacgaaaata cggaatcaaa atttgggttg
     3541 tggcaaagta ccaagcgata gagactactc caaaaagaat ggctgttgac cgccattctt
     3601 ttcgattgaa gaaaacaaac acagcccacc cgataccaaa agcaaataga gtttccttaa
     3661 aaagcaaaca gaaaataaac aaagcaatat gcagtctttt gttctttgaa atataaaagt
     3721 aaaacaacag agcaatgggc aacgtcgcaa tggtcacttc atgaaaatcc caccacagtg
     3781 cattttgtaa acctgcatat aaaaaatagg tcgcaagcac acaaaaacta gccaaagaac
     3841 ttttcagaac tcgcttgaca atcaaaacca acaccaatcc agagagagtt acaattgctg
     3901 cttgcgctac caaaatggtt tccgaccagg ggaatagcca atacagtggt gctaataaga
     3961 atattgcggg actaaaatga tcggcaaaga tgagcttgcc gccggccttg gaaaaatgat
     4021 caatgatcgg cggctgaaaa tgagctactt tccacagcgc agtatcaaaa atgccaaaat
     4081 cataatactg cgcatcatat tgccaaaatc tatgtagtga aattgcgcta ctgccaacaa
     4141 aaaaaatcac tgcgcagcaa ataattgcaa aagtccaaaa atgactcact ttcattgtca
     4201 gtattctata aaatattgca attatttgct gtaggaaagt cttaagtaaa ttctactttt
     4261 aattcgcaag aattaaatac acctgttcgt agataaaatc ggctttagaa acatcagcat
     4321 cagcttcaaa accagttatt gtgtctttaa agtttggatc ttcaactaac gccaagtatc
     4381 tatcttgagc ccgctgaagc acaagctctc caaatactct gagcgcaact aatggttggg
     4441 aagataatac cgcctgaatg agctcttcta taggcatctc ttctagcatt ttgagttcag
     4501 aggaaccaaa tcgaccagtt ttacttgttt cccatgtcat agtttcttct ccggtttata
     4561 gtataactaa ttatatcaca ttacatcatt ttaatttctg ctatactaag tccaaacctt
     4621 cagggcaggg tgaaattccc gatcggcggt agagcccgcg agccgagttc gtttgtttag
     4681 tagtagacag tgagcgacgc aggattcggt gcgtttagac tattagttta aacaactccg
     4741 aggccg
//
LOCUS       DAOQZP010000060         4734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_18348_length_4734_cov_4.627271, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000060 DAOQZP010000000
VERSION     DAOQZP010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18348_length_4734_cov_4.627271"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..693)
                     /locus_tag="PKH60_04350"
     CDS             complement(175..693)
                     /locus_tag="PKH60_04350"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNQ17303.1"
                     /translation="MTIIRLKIRETYHPVVAQIISLLEEHYYWFETFEHKPVRTSQEA
                     AKIRSGYSLEQGAKALIVRLKYSKNESKFVMLVLPGDARFDKQKVAEAFKTKDIRFAT
                     EAEVSEITSGVELGGVPPFGNLFGLEVIVDPSLLEHEKIIFNAGDRRFSIAMNTADYV
                     TLVQPKVVRIIL"
     gene            744..1124
                     /locus_tag="PKH60_04355"
     CDS             744..1124
                     /locus_tag="PKH60_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17304.1"
                     /translation="MSLSREQNKKGFREGALDKGSTIVAIEVAIGLLLIALVAKYGKT
                     LTLEIREKVKNLQTHKAELQSAIENDDVDVNFSTESAAHKAMFLNHLFLELADHAHVN
                     WSHQAIQVLTLLPELLRHHGPKEG"
     gene            1185..1949
                     /gene="radC"
                     /locus_tag="PKH60_04360"
     CDS             1185..1949
                     /gene="radC"
                     /locus_tag="PKH60_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNQ17305.1"
                     /translation="MPPSPKSVRKASVGVVVGTPKHVSRAKQKSSLPVSALPAYQRPR
                     ERLLEFGPTNITTAELLAIIIGHGTAGCGVLDLAQKAEAWLAQADLAPQPAKLSSALG
                     LSRALTLKLIAIQELCRRSQHPTLSPALTSPKLIVDQVTELIKSSQEHVLALYIDARF
                     HLLEKRTIAIGKQNSVQLEPRDIFAPALQIPAHGVVVVHNHPSGNPEPSADDLNFTQR
                     VLAASQLLGITLVDHIIVGNKSWYSFGEHGLHDQLW"
     gene            complement(1963..2307)
                     /gene="raiA"
                     /locus_tag="PKH60_04365"
     CDS             complement(1963..2307)
                     /gene="raiA"
                     /locus_tag="PKH60_04365"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNQ17306.1"
                     /translation="MQVLVSSKNFVVTEAIRAFAQEQADKLGHLSEKIIKVRLYLETV
                     EKKTNDPLANRAQVVVEIPGADITVEKRAVEMYEAVLKAFGVAHRHVRKHHEKLKTKR
                     RKAKRQEDEVLD"
     gene            complement(2391..3005)
                     /gene="recR"
                     /locus_tag="PKH60_04370"
     CDS             complement(2391..3005)
                     /gene="recR"
                     /locus_tag="PKH60_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010896220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNQ17307.1"
                     /translation="MQLLPKAVRQLIESFERLPGIGPKSAQRLVFYLLHNPEHELATF
                     ANRLLALKRETVLCTQCHSVSEKDPCHFCADQNRDQAVLCIVEEPLDTVAIENSGTFH
                     GLYHVLHGSIQPLNNIGPDQLFLYDMSKRLDGIEEIILATNPTMEGEATALFIVEYLK
                     KQMGDEHLPKITRIGHGLPVGADIEYADGVTLKRALEGRRAFYN"
     gene            complement(3043..4293)
                     /locus_tag="PKH60_04375"
     CDS             complement(3043..4293)
                     /locus_tag="PKH60_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17308.1"
                     /translation="MESLHSTSQPYEKVPYLSNLSLESRKALEREQFTVTTIKITDLG
                     SEMVVPILTWQGIERNVTDLRELFGEPILDIQVSTPEQTDDENPEQKSFLSQSKFNLG
                     DYTDAEFLERFFNVPEFFNQREVGQIVTSELSRGINQKLLGRLHAFYHADTSVGRATN
                     LDQPAPGLELTSREHMIWMVGNLQLDDLPVTSAQRRVARITAILHDWGKIVMSRNGQH
                     PEIGAAMARSILLLLHQQDPGLYPMELVNDVCWSINMHHLLERVFKPVKEESLNKISD
                     DSEVNQTLLTYNQADPNTMSAHLLKNSVKRSEEWYLYQAAKTNIKASNNEQVISVEQY
                     HSFMEYLKTLAYYEDIPESQHALRELTELLRIPDEKNIGLMLAMVRVIEADLVATDRP
                     PSETAFRLNQLQLVLQGILATAEA"
     gene            complement(4310..>4734)
                     /locus_tag="PKH60_04380"
     CDS             complement(4310..>4734)
                     /locus_tag="PKH60_04380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNQ17309.1"
                     /translation="VLGIKRFDVTLPLPEYKTSGAAGLDLVSRLDVSIEPQTVGYIPL
                     NIALELPEGYFAMIAARSSLHKKGLMLANGIGVGDYDYRGDGDEYTAALYNFTQQTVA
                     VTKGERLVQLLILPVARVEIQEKEVLGNPNRGGFGSTG"
BASE COUNT         1314 a         1072 c          929 g         1419 t
ORIGIN      
        1 aaagttgaat gaatttccgc aaaccatgac gttttagata gtcaatctct gcgtgggtaa
       61 tttctttgag agcagtgaag ttgagtagtt cctctggtaa atacagcaat agatgaagcg
      121 tctgagcaat cgtaccgtca aaatcaaaca atactgttgg ctgtttacta tttttcatag
      181 aataattcta acaacttttg gttgaaccaa ggtaacgtaa tctgcggtat tcattgctat
      241 agaaaatcgc cggtcgccag cattgaaaat tattttttca tgttcaagta aactgggatc
      301 gacgatgact tccaacccaa ataagttacc aaacggtggg acgccaccaa gttccactcc
      361 gctcgttatt tcacttactt ccgcttcggt agcgaaacga atatcctttg tcttgaatgc
      421 ttctgcaact ttctgtttgt cgaagcgggc atctcctggc aacaccaaca ttacaaactt
      481 actctcattt tttgaatatt tcagccggac aattagggct ttagcgcctt gttccaagct
      541 gtatccagat ctaattttag ctgcttcttg gctggtgcgg acaggtttat gctcaaaggt
      601 ttcaaaccaa taataatgtt cttctagtag agagataatc tgagcgacga ctgggtgata
      661 ggtttctctt attttcagtc tgattattgt cataatcaag ttattctata gtatgattat
      721 tgttctatat atgggtattg aaaatgagtt tatctcgcga acagaataaa aaaggcttcc
      781 gtgaaggtgc tctagataaa gggagtacta ttgttgctat tgaagtggca atcggcttgc
      841 tactgattgc tttagtagct aaatatggca aaacattgac cctcgaaata cgagaaaaag
      901 ttaaaaactt acaaacccac aaggctgaac ttcaatctgc gattgagaac gatgatgtgg
      961 atgtaaattt cagcactgaa agcgctgcgc ataaagcaat gtttttaaat catctttttc
     1021 ttgaacttgc cgaccatgca cacgtcaatt ggagtcatca agcgattcag gtattaactt
     1081 tactaccaga actgcttcga catcacggtc ctaaggaagg ctaacgtttt atttcaaaga
     1141 ttgttacggt taatgcaagg ttgtttcgta tagtactttc ttggatgcca ccctcaccaa
     1201 aatctgtccg aaaagcttca gttggggtag ttgtgggtac acccaaacat gtatcgaggg
     1261 cgaaacaaaa atcgagtctt ccggtaagcg ccttgcccgc ctaccaaaga cccagagaac
     1321 gcttgctcga gtttggaccg acaaatataa caacagctga actgctcgca attatcatcg
     1381 ggcacggcac cgctggctgt ggcgtactag atttggctca aaaagctgaa gcgtggcttg
     1441 cccaagcaga tctagcgcca cagccagcca agctgagttc agcgctcggc ctcagccggg
     1501 cgctgactct caaactcatc gccatccaag aactatgtcg acgctctcaa cacccaactc
     1561 tctctcccgc tctcacttca cccaaattaa ttgttgacca agtcacagag ttaattaagt
     1621 catcacaaga acatgtgctc gcactgtata ttgatgcgcg ttttcattta ttagaaaaac
     1681 gaaccattgc tatcggaaaa caaaattcgg ttcaactgga accgagagac atcttcgcac
     1741 ctgctcttca gatccccgct catggcgtag tagttgtaca taaccatcca tctggcaatc
     1801 ctgagccaag tgcagacgat cttaatttta ctcagcgggt tttagcagcc agtcagttac
     1861 ttggaattac actggtggat catattattg taggaaataa atcctggtat tcatttggcg
     1921 aacacgggtt acacgatcaa ctttggtaaa aaccagattt ttttaatcca aaacttcatc
     1981 ttcttgccgc ttagcttttc gccgtttcgt tttcaatttt tcatggtgct tacgcacatg
     2041 ccgatgggcg acaccgaagg cttttaacac agcttcgtac atttccactg ctcgtttttc
     2101 tacggtaata tctgctccag gaatctctac cacaacttgc gcacgatttg ctaacggatc
     2161 attagtcttc ttttctactg tctccagata cagtcgaact ttaattattt tttcagacaa
     2221 gtgacctaat ttatccgcct gttcttgggc gaacgctctg atcgcctcag ttacaacaaa
     2281 atttttagaa gaaaccaaca cctgcatggt ggtacctttc tggtaactga tacggagaat
     2341 ttgacgagcc cacaaagagc atcattagta tcaattgtat cacttccaaa ttagttatag
     2401 aaagcccttc ttccttcaag cgctcgtttg agtgttacac catcggcgta ttcaatatct
     2461 gctccaactg gtaaaccatg cccaatacga gtaatttttg gcagatgttc atcgcccatt
     2521 tgtttcttaa gatattcaac aataaacaat gctgtagctt cgccttccat cgtcggattt
     2581 gttgctaaaa tgatttcttc tataccatca agacgtttag acatgtcgta taaaaatagt
     2641 tgatctggtc caatgttgtt cagtggttga atactgccat ggagcacatg atacaaacca
     2701 tgaaatgtgc ctgagttttc tattgccaca gtgtccaaag gctcttcaac aatacagaga
     2761 actgcttgat ctcgattttg gtcagcacaa aaatggcaag gatccttctc gcttactgag
     2821 tgacactggg tgcataatac tgtttctcgt ttaagtgcca acaaacggtt ggcaaacgtt
     2881 gctagttcat gttctggatt gtgcagcaaa tagaacacca agcgctgagc cgacttgggc
     2941 ccaatacccg gtaagcgctc aaatgattca atcagttgcc gaacagcttt tggaagtaac
     3001 tgcataataa tcttcgcatt gtgccctgaa gtttcaaaag aatcaagcct cagccgtcgc
     3061 cagtattccc tgcaacacca attgcaactg atttaatcga aaagccgttt cagatggtgg
     3121 acgatcggta gccacaagat cagcttcaat cactcgcacc atcgccaaca tcaaaccaat
     3181 atttttttcg tcgggaattc tgagtagttc agtcaattct cgcagagcat gttgcgattc
     3241 tggtatatcc tcataataag caagagtctt taaatattcc atgaaactgt ggtattgctc
     3301 aacagaaatc acctgttcat tgttacttgc tttaatatta gtcttagcgg cttgatacaa
     3361 ataccattct tccgaccttt tgacgctatt ttttagtaaa tgagcgctca ttgtgtttgg
     3421 atcggcctga ttgtatgtaa gtaatgtctg attaacttca ctgtcatcgg aaattttatt
     3481 taacgattcc tcttttactg gcttaaatac tctttcaagt aagtggtgca tgttaataga
     3541 ccagcagaca tcattaacca gctccattgg atagagaccg ggatcttgct gatgcagcag
     3601 cagcaagatt gatctagcca tagctgcacc tatttccggg tgttggccat tgcgagacat
     3661 gacaattttc ccccaatcgt gcaaaatagc agtgatgcgc gccactctgc gttgtgcgct
     3721 agttactggc aaatcatcaa gctggagatt gcccaccatc caaatcatat gttctcttga
     3781 tgtcagctcc aacccaggtg ctggttgatc tagatttgtc gctctgccca cacttgtatc
     3841 tgcgtggtaa aaagcgtgta atctgcccag caacttttga ttgatacctc gagacaattc
     3901 tgaggtgaca atctgaccta cttctctctg attaaaaaat tctggaacat taaaaaatcg
     3961 ctctagaaat tctgcatctg tgtaatctcc taggttaaac ttagactgag aaagaaatga
     4021 tttttgttcg ggattttcat catcagtctg ctcgggggta cttacttgta tatccaagat
     4081 tggttcgcca aaaagctctc gtaagtctgt tacatttctt tcaatccctt gccaagtaag
     4141 tatgggaact accatctccg aacccaaatc agtaatttta atggtagtta ccgtgaattg
     4201 ctctctttct agtgcctttc tactttcaag agataggtta gataaatatg gaactttctc
     4261 atatggttga cttgttgaat gaagtgattc catagagtgc tgcattctgt taacctgtcg
     4321 atccaaaacc accgcggttt ggattaccga gtacttcttt ttcttgtatc tccactcgcg
     4381 caacgggcaa tatcaatagt tgaaccagcc gctctccttt ggtaacagca acagtttgtt
     4441 gcgtaaaatt atagagagcg gcagtgtact catcaccatc tccccgataa tcgtagtcac
     4501 ccactccaat gccatttgcg agcatcaaac cttttttgtg caaggaactt cgcgctgcaa
     4561 tcatcgcaaa ataaccctcg ggtaattcaa gagcaatatt gagagggata taaccaactg
     4621 tttgtggctc gattgaaaca tctagtcggg acaccaaatc taacccggct gcaccagacg
     4681 ttttatattc aggcaacggc agagttacat caaagcgttt tatccctagc acta
//
LOCUS       DAOQZP010000061         4693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_18556_length_4693_cov_5.392626, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000061 DAOQZP010000000
VERSION     DAOQZP010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18556_length_4693_cov_5.392626"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /gene="infC"
                     /locus_tag="PKH60_04385"
     CDS             <1..520
                     /gene="infC"
                     /locus_tag="PKH60_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNQ17310.1"
                     /translation="QSQTVFVVANNRIRTPQVRVIDDQGNMLGVMDTHVAMNQAYAED
                     KDLVMVNDQQQPPIAKIIELNKYKYQLQQKQAASRKKAKAQDTKEVRFSPFMSDGDFE
                     SKLKKVKDFLEDGDKVKLTLFFKGRQITKKSFGDQVIARVIEATAEEAVVEIVPKLIG
                     KKIIAQLMPKKK"
     gene            526..729
                     /gene="rpmI"
                     /locus_tag="PKH60_04390"
     CDS             526..729
                     /gene="rpmI"
                     /locus_tag="PKH60_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNQ17311.1"
                     /translation="MKKNKLKTRKGVAHRFKITGSGKVLRRSQNMRHLRRKKSKKTIR
                     AYRIPIEVKGKWAIKIKKMLGIG"
     gene            734..1087
                     /gene="rplT"
                     /locus_tag="PKH60_04395"
     CDS             734..1087
                     /gene="rplT"
                     /locus_tag="PKH60_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNQ17312.1"
                     /translation="MPRTKTGTTRHHRHKKVLDSNKGFRGANHRLYKRAHEALLHAGQ
                     YAYIGRKLRKRDFRKLWITRLSAAIKEVSAELNYSRVINLLKKANIQLNRKMLSEIAI
                     RDFETFKRIITQARG"
     gene            1179..2273
                     /gene="pheS"
                     /locus_tag="PKH60_04400"
     CDS             1179..2273
                     /gene="pheS"
                     /locus_tag="PKH60_04400"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNQ17313.1"
                     /translation="MTTELNNFMSLVQNLTEELEKTTDLSGQSLLQVYLGKKGKFAAI
                     SSELTKLPITDRAEAGQALNKLKTVIQQKIELAKNTTAEEWVDLSAPGIQPKIGHLHP
                     ITQAIEEISAIFSRIGFTRVRYPEVDWDWYVFESLNMPPNHPARDEWETFFIDAPDTS
                     QPVAYDPNQPRLRHPSLIGGKQVLTTHTSNGQVREMERLDSQPPIRMINIARCYRRQQ
                     DATHTQMFHQFEGLVIDTDITIQHLKGTLEYFAKEFYGPNVKSRIRPFHFRFTEPSFE
                     VDFSCHICKGTGQIDGEKCKLCKSGWHEVGGAGMVHPNVLKAGGIDPQKYTGFAFGWG
                     VERTYTLKPNLNIDDIRLLYSADLRFLEQF"
     gene            2275..4302
                     /locus_tag="PKH60_04405"
     CDS             2275..4302
                     /locus_tag="PKH60_04405"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNQ17314.1"
                     /translation="MNILIPHSWLLDHLDTDATPQEIQELLSLSGPSVERVIEKDGEF
                     IYDMEITTNRVDSLSVRGIAREAAVILKQNNKKAELRPLQLPTPKPTQTQLPLPKITN
                     DPNVNGRTICIILANVQQTATPDWMAHRLRLIDLNVHNSAIDITNYVTHDLGHPCHAF
                     DYDKLMATGGEINIVEAQPNETFMTLDGLRFTTVGGEVVFKNGKNEIIDLPSIKGTAN
                     TSIDSTTKNVLLLMESIRADKVRFASMTHNIRTVAAQLMEKSVDPNLAQDVLFKACQQ
                     YQELCSARIASEVYDDFPAKANQALVHFPLSELTRYLGMTIPTTTVTSILSDLGCLVT
                     TQNDSVIDVTPPTYRPDLTTPADIVEEVARMYGYQNIPNQLMASALPLNPPKDQRFDL
                     EAKLKTFLAHTGWQEVYTYSMVSETLALASGFSLEDHVRILNPLSDDRVYLRRSLIPS
                     LIEALDANPLQPQLATFELAHAYHPVPNQLPIEELRLTLVSRRPYREVRGIIESLLSQ
                     LHLDQDYVIFSAISQDFVHNEQISTPSDTLLIGQIEFKGSNQTLLLGQVSILKTNVVA
                     IDLDVASVFSVARSHPIYHPIAKTSPLVEDLTFTFPKHTPIGEVIKQMKEEIAEISNI
                     QLASSFRNNVTFTIKYFDPKINMSNDRVEPLRQQLVYAVEKKWQASLVGTI"
     gene            4335..>4693
                     /locus_tag="PKH60_04410"
     CDS             4335..>4693
                     /locus_tag="PKH60_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17315.1"
                     /translation="MVLLIFLLPTNLFLTFGKDSAYVTGMLSDYLIGKIYLSEVVLWL
                     YIGSIVLIKRSELVQWFSNISKRIILNRPSVFLLLLSAIGLLLRQFWTDHATIGLWNL
                     FQWLEIILLGVALRFHIS"
BASE COUNT         1520 a         1051 c          911 g         1211 t
ORIGIN      
        1 tcaaagccaa accgtctttg tcgttgctaa taatcgaatc agaacacccc aagtgcgggt
       61 gattgatgac cagggaaata tgctgggagt catggacaca catgttgcca tgaaccaagc
      121 ctacgccgaa gacaaagacc tagtcatggt caatgatcag cagcaaccgc caattgccaa
      181 gatcattgaa ctcaacaaat ataagtatca gctgcaacaa aaacaggcag ccagtagaaa
      241 gaaagcaaaa gcacaggata caaaagaagt tcgtttcagc ccattcatga gcgatggcga
      301 ctttgaatca aaattaaaaa aagtcaaaga tttccttgaa gatggcgaca aagttaaact
      361 gacgttattc ttcaaaggca gacaaattac gaaaaaatca ttcggtgacc aagtaattgc
      421 cagagtcatc gaggccacgg ccgaagaagc agtagtcgaa attgtgccaa aattgatcgg
      481 taaaaaaatc atcgctcaat taatgccgaa gaaaaagtaa gacgtatgaa aaaaaacaaa
      541 ctaaaaactc gtaagggtgt agcacatcgt ttcaaaatca caggcagtgg caaagttttg
      601 agacgctctc agaacatgag acacctgaga agaaaaaaaa gcaagaaaac tattcgagcc
      661 taccgcattc caattgaagt gaaaggaaag tgggcaatta aaattaaaaa aatgttagga
      721 atagggtaaa catatgccac gaacaaaaac aggtaccaca cgccatcatc gtcataaaaa
      781 agttttggac agcaataaag gcttccgtgg tgcaaaccac cggttataca aacgtgctca
      841 tgaagcttta ctacatgctg gtcaatacgc ctacatcggc cgaaagctgc gcaagcgtga
      901 cttccgcaag ctttggatta cccgcttgtc ggcagctatc aaagaagtaa gtgcagagct
      961 caactactcc agagtaatta atctgttaaa aaaagcaaat attcaactca atcgcaaaat
     1021 gctttctgaa attgccattc gggattttga aacgtttaaa cgcataatta cacaggcgag
     1081 gggataatcg caatccaacc aatcacgtag tttgaaaatc accccgccgc acatcatacc
     1141 ggcggggttt ttgatgctgt acaatgaaaa gcacacctat gacaaccgag ttaaacaact
     1201 tcatgagtct ggttcaaaac ctaactgagg agttagaaaa aaccaccgat ctctctgggc
     1261 aatctctttt acaagtgtat ttgggtaaaa aaggaaagtt tgcagcaata tcgagtgaac
     1321 tgactaaact accaatcaca gatcgcgcag aggctggcca ggctttaaac aaattgaaaa
     1381 ctgtcattca acaaaaaata gaactggcaa aaaacactac tgcagaggag tgggttgatc
     1441 tgagtgctcc tgggattcaa cccaaaattg gacatttaca tccaattact caagcgattg
     1501 aagaaatttc agcgattttc agccgaatcg gctttacccg cgtacgatat cctgaggttg
     1561 actgggactg gtacgttttt gaaagcctca atatgccccc caatcatcct gcacgtgacg
     1621 aatgggaaac tttttttatc gatgcaccag acacttctca accagtagca tatgacccca
     1681 accaaccaag attgagacat ccaagcttaa ttggcggcaa acaagtcctt accactcata
     1741 catcaaacgg gcaggtgaga gaaatggaac gtttagatag ccaaccacca attcgtatga
     1801 tcaatatagc tcgctgctac cgccggcaac aggacgctac tcacacccaa atgtttcatc
     1861 aatttgaagg tttggtgatc gataccgata taaccattca acacttaaaa ggcacgttgg
     1921 aatactttgc aaaagaattt tatggtccca acgttaaaag taggatcaga ccatttcatt
     1981 ttcgttttac tgaaccatca tttgaagtcg acttttcctg ccatatttgc aaaggaactg
     2041 gccaaatcga cggagaaaaa tgcaaactct gcaaatccgg ctggcatgag gtgggaggtg
     2101 caggcatggt ccatcccaat gtcctaaaag ccggcggaat cgacccacaa aaatacaccg
     2161 gatttgcttt tggttgggga gtggaacgaa cttatacgct caagccaaat ctcaatattg
     2221 atgatattag acttttgtat tcagccgacc tgcgattttt agaacaattc tgatatgaac
     2281 attctcattc cacattcctg gctacttgat caccttgata ctgacgctac tccgcaagaa
     2341 attcaagaat tgctatccct atctggacct tcggttgaaa gagttattga aaaagacggt
     2401 gagtttatct acgacatgga aattacaacc aatcgggtgg actcactttc tgttcgagga
     2461 attgcaagag aggcggctgt tattctaaaa cagaataata aaaaagcaga attacgcccc
     2521 ctacaacttc ctactcccaa gcctacccaa actcaactgc cacttccaaa aattacgaac
     2581 gatcccaatg taaatggcag aactatttgc attattttgg ccaatgtaca acaaacagca
     2641 actccagatt ggatggctca tcgtctgaga ctcattgatt tgaatgtcca taacagcgca
     2701 attgatatca ctaattatgt gacacatgat ttgggtcatc cttgccatgc ttttgattat
     2761 gacaaactga tggctactgg tggagaaatt aatattgttg aagcacaacc caatgaaacg
     2821 tttatgacgc tagatggttt acgttttacc accgtgggtg gagaagtagt ctttaaaaac
     2881 ggcaaaaatg aaattatcga tttgccttca atcaagggaa ctgccaacac ttcaattgac
     2941 tcaaccacaa aaaatgtgtt gctactgatg gaatctatta gagcagacaa ggttcgtttt
     3001 gcgagcatga cccacaacat tcgcaccgtg gctgcgcaac tcatggaaaa atcggttgat
     3061 cccaatctag cacaagacgt gctcttcaaa gcctgtcagc aatatcaaga actgtgttct
     3121 gcccgaatcg ccagcgaagt ctatgacgat tttcctgcaa aagccaacca ggctctcgtt
     3181 cactttccat tatcagagct gactagatat cttgggatga ctatacccac tacgactgta
     3241 acgagcatcc tctcagacct tggttgcttg gtaacgactc aaaacgactc agtcattgat
     3301 gttacccctc caacctatcg accagactta accacgccag cggacattgt ggaagaagta
     3361 gctagaatgt atggctatca gaacataccc aatcaactca tggcttcagc gttgccactc
     3421 aatccaccca aagaccaacg ctttgatttg gaagcaaaac taaaaacctt tttggctcac
     3481 acaggttggc aagaggtata tacctattct atggttagcg agacccttgc acttgcctcg
     3541 ggtttttcgt tggaagatca tgtacgcata cttaaccctt tgagtgatga tcgcgtctat
     3601 ttacgtcgat ctctaattcc ctcattaata gaagcactcg atgctaatcc gctacagcct
     3661 caactagcta cgtttgaact ggcacacgcc tatcacccag tcccaaatca acttcccatc
     3721 gaagaacttc gcttaaccct agtcagtcgg cgaccatatc gagaagtcag ggggattatt
     3781 gagtctttgc tatcacaact acatcttgat caagattatg ttatttttag tgcgataagt
     3841 caagattttg ttcataatga gcaaattagc actcctagtg acactctgct aattggtcag
     3901 attgagttca agggttcgaa ccagactctc ttgctcggtc aagttagcat cttaaagacc
     3961 aatgtcgtcg caatcgatct tgatgtagca tcagtatttt cggttgctcg ctcacatcct
     4021 atctaccatc caattgctaa aaccagccca ttggtagagg atctcacatt tacatttcca
     4081 aaacacactc cgattggcga ggttatcaaa cagatgaaag aggaaattgc agaaatttca
     4141 aatattcaac ttgcaagcag ttttcgcaac aacgtcactt ttacaattaa gtattttgat
     4201 cctaaaatta acatgtcaaa cgaccgagta gaacccctgc gtcaacaatt ggtctacgcc
     4261 gtagaaaaaa aatggcaagc atctctggtt ggcaccatct aactacaatg aaacctcagt
     4321 gggctaacct atctatggtt cttctaattt ttttgcttcc cactaatttg tttttgacct
     4381 ttggaaaaga ctcggcttac gtcactggca tgctgagtga ttatttaatc ggaaaaatat
     4441 atctatcaga agtagtacta tggttatata ttggatcaat agtattaata aaaagatcag
     4501 aactagtaca atggtttagt aatatatcta aaagaattat tctcaacaga cccagcgtgt
     4561 ttttactact cctatcagcc ataggattac tattgaggca attttggact gaccacgcca
     4621 ccataggcct gtggaatctt tttcaatggt tagaaatcat tttgttagga gttgcgttac
     4681 gcttccatat ttc
//
LOCUS       DAOQZP010000062         3883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_24042_length_3883_cov_5.171891, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000062 DAOQZP010000000
VERSION     DAOQZP010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24042_length_3883_cov_5.171891"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2640)
                     /locus_tag="PKH60_04415"
     CDS             complement(<1..2640)
                     /locus_tag="PKH60_04415"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNQ17316.1"
                     /translation="MFLEKYKQTLLRWWHAIKHKLGLGARSRRSLMRVSQMKRQLVWM
                     KMFRYAALLSLVFVILGTVVFLGMLAFYAKDLPKSGEVIQRSGFSTKIFDRNGKLLDD
                     VYSDENRTFVPLSAIPQSLRDASIAIEDKEFYQHQGIDLFVFVRMPYYLITERRIVGG
                     STLTQQLVKKALLSDERTLPRKIKQVVLSLLVEQRFSKDEILEMYLNEVPYGGTAYGV
                     GAAAEYYFGKPVQELSMVESAILAGLPQRPTAYSPLAGKTNDAGEPLWKERAKGVLRR
                     MSEDNYITALAYEDALSQLETITFEGKRSAMVAPHFVFYTLSQLEEMYGPELVQSGGL
                     QVTTSLNLDLQNEAQTIVSEEIEKVTNLNITNGSVMVMNPNTGEILAMVGSRNFNDEE
                     IDGQFNVAVDGLRQPGSSIKPVVYLTLLRLGYTPASMMIDVETTFQRNENEKPYQPKN
                     YDGKFRGPVSLRNSLGSSLNVPAVKGLAKVGLETFLTQAYAMGFVTLEPTQENLQRLG
                     LSVALGGGEVHLIDSTTSFSSFANGGRRVEPVSILKVVDKNGTVLYEHRAVTGEEVMS
                     PEEAYLINHILSDDSARSMAFGANSKLNVSPNVAVKTGTTNDQRDNWAIGWSREVIVS
                     AWVGNNDNSAMKTVASGISGATPIWQRIIQYALKNGFTAPAWEVPAGVEQVSVDAVSG
                     YPSHDDFPAKTEVAIKGLLPSSPDPIHLKLKLCKGQTKLATDAQVVSGNFDEREFVRL
                     AEADPYSQDGINRWQQAIDAWVAPQPSDFYKPPTEYCGDQSDVSAVMERPENEKKYDS
                     ENIEFVVRADSGAGIEKIEIIADDKVIETVENREYRGTIKLKKGRYTVWVKAYARDGK
                     TKESEKKKIGTGGEDWQVPSPTPT"
     gene            2677..3879
                     /gene="tyrS"
                     /locus_tag="PKH60_04420"
     CDS             2677..3879
                     /gene="tyrS"
                     /locus_tag="PKH60_04420"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNQ17317.1"
                     /translation="MSTRELITDHNRIIEVLTRGVEQVLPSLDSLAKIMSEKRIRLYL
                     GIDPTGSELTLGHSVVLRKLQQFADLGHEVILLVGNGTVRIGDPTGRDSTRPELTDEQ
                     IMVNFAEWQQQASKVLNFDTIEIRKNGDWLDKLNYIEMIKLLANTTVQQLLERDMFQK
                     RLKAGLPIFGHEIIYPLIQGYDSVVMDVDLEIGGSDQLFNMMMGRTLQKNHRGKDKWV
                     LTTPIINGTDGRKMSKSFNNYIALTEPAESMYGKTMSILDEQIVPYFTVLTNTPQSEI
                     DQISHEIQSGENPMQYKKRLALAITELYHGTDAALQAQGYFERVVQQQDVPEEIPTIH
                     VKNQAVTWLDLVGICYPQLSRSDRRRLIEQGGVKLNDQKITEPLTTASYSSGDILKIG
                     KRGYFKLQ"
BASE COUNT         1112 a          891 c          780 g         1100 t
ORIGIN      
        1 ggttggagta ggggagggta cttgccaatc ttctccacca gtgccgattt tcttcttttc
       61 actttcttta gtttttccat ctcttgcata tgctttaacc caaacagtgt atcgaccttt
      121 ttttaactta atcgtgcctc gatattcccg attttcaacg gtttcgatca ccttgtcatc
      181 agcaataatt tctatttttt cgatgcctgc tccagaatct gccctaacta caaattcgat
      241 attctcagaa tcatattttt tttcattttc cggacgctcc atcactgcag aaacatctga
      301 ttgatcacca caatattccg tgggtggttt ataaaaatcg cttggctgag gtgccaccca
      361 agcatcgata gcctgctgcc aacgattaat cccgtcctgg ctgtatgggt ccgcttcagc
      421 caagcgaaca aattctcttt cgtcaaagtt gccagaaacc acctgagcat cagtggctaa
      481 cttagtttgt cctttacaaa gttttaactt gagatgtatc gggtcaggag aactcggcag
      541 taatcccttg atagcgacct ctgttttggc aggaaaatca tcatgtgatg gataccccga
      601 tactgcatca acagaaactt gttccacacc ggcgggaact tcccaagcag gtgcagtaaa
      661 accattcttc agcgcatact gaattattcg ttgccagatt ggggttgcac cagaaatacc
      721 tgatgctact gtcttcatgg cgctgttatc gttgttaccc acccaggcac tgacgattac
      781 ttcacgactc caaccaattg cccagttatc tctttgatca ttagtggtac cggtcttgac
      841 tgcaacattt ggactaacat taagttttga attggcacca aatgccatcg accgcgctga
      901 atcgtcagac aaaatgtgat tgattaggta tgcttcttct ggtgacatga cttcttcgcc
      961 agtcaccgct cgatgctcat ataacacagt tccattctta tccactacct ttaaaataga
     1021 cacaggttca acccttcttc ctccattcgc aaatgaacta aatgaggtag ttgaatcaat
     1081 caaatgcact tcgccgccac ccaaagctac tgagagacca aggcgctgta aattttcttg
     1141 agttggctct agggtcacaa aacccattgc gtaggcttgg gttaaaaagg tttctaaacc
     1201 aactttggcc aatcccttca cagcaggaac gttaagcgaa ctaccaaggc tattgcgcaa
     1261 actgactggg cctctaaatt tcccatcata attctttggc tgatacggtt tctcattttc
     1321 atttcgttga aacgttgttt caacatcaat catcatcgat gcgggtgtat agcccaaccg
     1381 cagtagggtc aagtacacca cgggtttaat tgaacttcct ggctgacgca atccatcaac
     1441 cgccacatta aattgtccat caatttcttc atcattaaaa ttgcggcttc caaccatagc
     1501 caaaatttca cccgtgtttg gattcatcac catgactgat ccgtttgtta tgttgaggtt
     1561 tgtcaccttt tcaatttcct cactcacaat cgtttgggct tcattttgca ggtctagatt
     1621 aagactggta gttacctgta atccaccact ttgaacaagt tcgggaccat acatctcttc
     1681 taactgactc agtgtataaa aaacgaaatg aggagccacc atcgcgctac gcttgccttc
     1741 aaaggtaatt gtttctaact gagacagcgc atcttcatac gctagcgcag ttatataatt
     1801 atcttcactc attcttctga gaaccccttt tgctcgttct ttccataatg gctcacctgc
     1861 atcattagtt ttaccggcaa ggggagagta ggcagtaggt ctctggggta aaccagcaag
     1921 aatggcggac tcaaccatgc tgagctcttg gaccggcttg ccaaagtagt attcagcggc
     1981 tgccccaaca ccataggccg ttcccccata cggaacttca ttcagataca tctcaagaat
     2041 ctcatcttta gaaaagcgtt gttctaccaa caatgacaac acaacttgct taatttttcg
     2101 aggtagggtt cgttcatcac taagtagggc tttttttact aactgctggg taagtgtgga
     2161 tcctcccacg attcgacgct cagtgatgag atagtaaggc atacgaacaa agacaaacag
     2221 atcgatgcct tggtgttgat aaaattcttt atcttcaatt gctatcgatg catcacgaag
     2281 tgattgggga atggcagaaa gtggaacaaa ggtcctgttt tcatccgaat agacgtcgtc
     2341 aagtaatttg ccattacgat caaaaatctt agtgctgaat ccagatcttt gtatgacctc
     2401 accagatttt ggtaaatcct tggcgtagaa ggctagcatg ccaagaaaaa ccacagtgcc
     2461 gagaattaca aaaacaagtg atagcaatgc tgcgtaacgg aacattttca tccaaactaa
     2521 ctgtcgcttc atttgtgata ctcgcataag gctcctgcgc gaacgagcac ccaaaccaag
     2581 tttgtgtttt atggcatgcc accaacgaag caaggtttgt ttgtattttt ctagaaacat
     2641 tgctctattg tatagcattc atgctacaat tattcaatgt ctactcgaga actcataacc
     2701 gatcacaacc gcataattga ggtgttaact cgaggtgttg aacaagtgtt accctcacta
     2761 gattccctcg caaaaattat gtccgaaaaa cgaattcgtt tgtatcttgg aattgatcct
     2821 actggtagcg agttgacgct agggcatagc gtagtcctgc gtaaattgca gcaattcgca
     2881 gacctaggcc atgaggtaat tttactcgtg ggcaacggca ctgtcaggat cggtgatcca
     2941 accggcagag attccactcg accagagctc acagacgaac aaattatggt taactttgct
     3001 gagtggcaac agcaggcaag caaagtactc aattttgaca ccattgaaat tagaaaaaat
     3061 ggcgattggc tggataaact gaactatatt gaaatgatca aattattagc caatacaact
     3121 gtccaacaat tgttagagcg tgacatgttc caaaaacggc tcaaggctgg cctaccaatc
     3181 tttggtcatg aaataatcta tccgttgatt caaggctatg acagcgtggt aatggatgta
     3241 gatttagaga ttggtggttc cgaccagctt ttcaacatga tgatgggtag gacgcttcaa
     3301 aagaatcatc gtggcaaaga taagtgggtg ctcacaacac cgattatcaa cgggaccgat
     3361 ggcagaaaga tgagtaagtc gtttaataac tacattgctc ttacagagcc agctgaaagt
     3421 atgtatggca aaaccatgag tatccttgat gaacaaatag tgccgtattt tactgttctc
     3481 accaatactc cacagtcaga aattgatcag atttcacatg agatacagtc tggcgaaaac
     3541 cctatgcagt acaaaaaacg attagctctc gcaatcactg agttgtacca cggaacggat
     3601 gctgcccttc aagctcaagg ttattttgag cgagtcgttc aacaacaaga tgtgccagag
     3661 gaaattccaa caattcatgt taaaaaccaa gcagtaacat ggctcgactt agtcggaatt
     3721 tgttatcctc aattgagtcg gagtgatcgc cgaagactga ttgagcaggg cggggtaaaa
     3781 ctcaatgatc aaaaaataac cgagccgctc acaacagcaa gctattcaag tggtgatatt
     3841 ttaaaaattg gtaaacgagg atattttaaa ttacaataga aag
//
LOCUS       DAOQZP010000063         3872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_24152_length_3872_cov_5.216924, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000063 DAOQZP010000000
VERSION     DAOQZP010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24152_length_3872_cov_5.216924"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="PKH60_04425"
     CDS             <1..855
                     /locus_tag="PKH60_04425"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNQ17318.1"
                     /translation="LAFIPRFTTPKAQADQVAKVKKQGARVFAAIGLRDDYLDRARYV
                     INAGADGLLLDVAHAHMLKTLKAIPKIKKAFSVPLIAGTVATYQGAYDLFAAGADTIK
                     VGIGAGSICITRIMAGSGVPQITAIMEANRAKKQFKNKFIMADAGMKNSGDIVKALAA
                     GASFICTGSLVAGTDEAPGKIIKRADSFYKEYNGSTSLLEKKRELTKHDGHSPDFTLH
                     VEGVSGIVRYKGPVADVIAGLCAGIRSGFSYSGSKNIKQLWKKAQFIQITSAGMYESR
                     AHDVLETR"
     gene            complement(869..1942)
                     /locus_tag="PKH60_04430"
     CDS             complement(869..1942)
                     /locus_tag="PKH60_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNQ17319.1"
                     /translation="MNTTTPGEMTRREFLKQSPTYLGAAVVGAATLPGALVAGIMENN
                     LMLDYVDNGPIAEGQPIEVIISDNLTVTFLPLEHSHSDLGYQFESVDLAKDTLTSLLQ
                     MPQSELWLEALPWEMEQMADFLPPEVATTIERIIPFNRRLIEYSNKVLQIAKDAKKRV
                     GDDLEVVCPEIVGGSPLFFLAIDKPPFGGLWIAYMVAMNVLVRKGFKQASEVSLALTE
                     TNINRRTLLSLGLRSTLLGLLTPSVMMSLAHMYQIFTQRPRQNHATEDSIRECWQAFS
                     LLEHEKVLASNDAKRHIVYISTPPHVEAILDRLRNQEELMREMKVYFAMFPDEIFEQG
                     NRRYKYIDTTDSWTMMDSNSGIS"
     gene            complement(2020..2238)
                     /locus_tag="PKH60_04435"
     CDS             complement(2020..2238)
                     /locus_tag="PKH60_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17320.1"
                     /translation="MKNEIYTNQEPEVTQYWRGLTRIDGVTRAVIDVLGNLVIVDTIE
                     FATKRVYSTDLIWYSYDQNLSYFIPDRS"
     gene            2320..>3872
                     /locus_tag="PKH60_04440"
     CDS             2320..>3872
                     /locus_tag="PKH60_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNQ17321.1"
                     /translation="MPALKSILDIDWNVTLDRQFSPSPQSWSDQVMYFLLVDRFSDGN
                     EKPELLFTADKAGSAIRTENHAERWRAAGYTFCGGTLKGARSKLEYLHQLGITTIWLS
                     PVLQQCRYDATSYHGYGAQNFLETDPHFGSKEDLRTFVDEAHQLGMFVVLDIVVNHTG
                     FVFEYEVPVNSETEPSFKVDGQHPVKYFHDQEGKLTIPFGPVDLDQSPEAWPYGAIWP
                     RELQSANTFNRRGCIGDWENPDEYLHGDFKFLKDINMGEGENQDFQPSEALIVLTEVY
                     QYWLAYADLDGFRLDAVKHMGVQAAKYFTKAIQQFAKNVGKDNFYIFGEIPGGEQQAR
                     ETMDQSGLSAALALLDIPSSLEAMIKGENNPWEYFKHFSKSNMVSGSNAGEIVWQKDE
                     IVLFLDDHDQIRRNFDKARFPAGSDEWSRLAFSALATLVCSAGIPCIYYGSEQGFDGQ
                     GRHDRYIRETMFASEFGAFRSRGCHCFNERHAIYQQLQRLLAIRKHLPALRQGDQQLL
                     EISGDGEHFG"
BASE COUNT         1104 a          765 c          872 g         1131 t
ORIGIN      
        1 ttggcgttta ttcccaggtt taccacccca aaagcgcagg cagaccaggt tgcaaaagtg
       61 aaaaagcagg gcgcccgcgt ctttgctgcg attggcttgc gtgatgatta tttggatcga
      121 gctcggtacg taatcaacgc gggcgctgat ggtttgctac ttgatgtggc acacgcccac
      181 atgctcaaaa ctctcaaggc aattcctaaa attaagaagg ccttttcagt acccttgatt
      241 gctggtacgg tggcgacata tcagggagcg tacgatctct ttgcggcggg tgcggataca
      301 attaaggttg ggattggcgc cggttctatt tgcattactc gcattatggc tggtagcggt
      361 gttccccaga taacagcgat tatggaggcg aatcgagcca agaaacaatt taagaataaa
      421 tttataatgg ccgacgctgg tatgaaaaat tcgggtgata ttgtgaaagc actagcagct
      481 ggtgccagct ttatttgcac cggttcatta gtggctggca ccgacgaagc cccagggaaa
      541 attattaagc gtgctgatag tttttataaa gaatacaatg gatccacttc cttgcttgaa
      601 aaaaaacgag aactcaccaa acacgatggt cactctcccg attttaccct tcatgtagag
      661 ggtgtgtcag gaatagttcg ttataagggt ccagtggccg acgtaattgc aggattatgc
      721 gctggtattc gctctggctt ctcctattct ggttcaaaaa acattaagca actttggaaa
      781 aaggctcaat tcattcaaat caccagtgct ggtatgtacg aaagtcgcgc tcacgatgta
      841 cttgaaacaa gatgatctac agattcaact atgagatccc tgagttggaa tccatcatgg
      901 tccagctatc ggttgtatca atgtatttgt atcttcgatt tccttgctca aatatttcat
      961 ctggaaacat tgcgaaatac accttcattt ctcgcataag ttcctcttga ttgcggagcc
     1021 gatcaagaat tgcttcaaca tgcggtggag tcgaaatata cacaatatga cgttttgcat
     1081 cgtttgacgc caatactttt tcatgttcaa gtaaggaaaa tgcttgccag cactctctaa
     1141 tactatcttc agtagcgtgg ttttgtcgtg gtctttgtgt aaatatttgg tacatatgtg
     1201 caagtgacat catcacagat ggcgtaagca gtcctagtaa agtagatctt aatcctaaac
     1261 tcaagagtgt gcgacggtta atgttcgtct cagttagtgc caaactaact tctgatgctt
     1321 gtttgaaccc ctttctaacc aagacgttca tagccaccat ataagcgatc caaagtccac
     1381 caaatggggg tttatcaatt gcaagaaaaa acaaaggtga gccgccgact atttcgggac
     1441 aaacaacctc aagatcatct ccaacccgtt ttttagcatc ttttgcgatt tgtaatactt
     1501 tatttgaata ctcaattagt cgacggttaa atggtatgat tcgttcgata gttgtcgcaa
     1561 cctcgggcgg taaaaaatca gccatttgtt ccatttccca agggagtgct tcaagccaga
     1621 gctctgactg tggcatttgt aaaagtgagg ttagcgtatc ctttgctaaa tcaacgcttt
     1681 caaactgata accaagatcg ctatgcgagt gttctaacgg tagaaatgtt acagttaggt
     1741 tatcactaat tataacttcg attggttggc cctcagcaat tgggccatta tcaacataat
     1801 caagcattaa attattttcc ataatgcctg caacaagtgc tccaggaagt gtggcggcac
     1861 caacaactgc agctcctaag taagtgggag attgctttag aaattctctc ctcgtcattt
     1921 ctccaggtgt tgttgtattc atcagatgta attattgaaa agtcattatt aaaagttact
     1981 taagattaat aatacaacat ttttagataa gtggtgattt taacttctat caggtatgaa
     2041 atagctcaaa ttttggtcat aagaatacca aatcaaatct gtggaataaa ctctttttgt
     2101 ggcaaactca atagtatcga caattactaa atttcccagc acatcaatta cagctcgagt
     2161 aacgccgtcg attctggtta atccacgcca gtactgggtt acctcaggtt cttgattagt
     2221 gtatatttca tttttcattg atctggatta taccagtaag ataccttgca gaattcaaac
     2281 cctattcagt gagcgagatt tatcagataa tgactatata tgcctgccct gaagtcaatt
     2341 ctagacattg attggaatgt aacgcttgat cgacaatttt ctccttcacc gcaatcatgg
     2401 agcgatcaag ttatgtattt tttgttagtt gatcgatttt cagacggaaa tgaaaaacca
     2461 gaattattat ttacagcaga taaagctggt tcagccatac gaacagagaa ccatgccgag
     2521 cgttggcgtg cagctgggta tacattttgt ggtggtacat tgaaaggcgc tcggtcaaaa
     2581 cttgagtatt tgcaccaatt aggtattacc accatttggc tgagtcccgt gttacagcaa
     2641 tgccggtacg acgccaccag ttaccatggg tatggagcgc aaaatttttt agaaaccgat
     2701 cctcattttg gtagtaaaga agatctcagg acatttgttg atgaggctca tcaacttgga
     2761 atgtttgtgg tgctagacat agtggtcaat catactgggt ttgtttttga atacgaagtt
     2821 ccagtgaata gcgaaacaga accaagtttc aaagttgatg gtcagcatcc tgtcaaatat
     2881 ttccacgatc aggaaggaaa actgacaatt ccatttggtc cagttgatct agaccaatcg
     2941 ccagaagcat ggccgtacgg agctatttgg cccagagagc tccagtcggc taatactttt
     3001 aatcggcgag ggtgtattgg tgactgggag aatcctgatg agtaccttca tggcgatttc
     3061 aaatttctta aagacatcaa tatgggcgag ggagagaatc aggactttca accttcagaa
     3121 gccttgatag tattgacgga ggtgtatcag tactggctag cgtacgcaga tttggatggc
     3181 tttcgacttg acgctgtgaa acacatggga gtacaggcag caaagtattt tacaaaagct
     3241 atacagcaat ttgccaaaaa tgttgggaaa gataattttt atatttttgg tgaaattcca
     3301 ggtggagagc aacaggccag agaaaccatg gaccaatcag gactaagtgc tgcattagct
     3361 ttgctagata ttccctccag tctcgaagcg atgattaaag gcgaaaataa cccctgggaa
     3421 tacttcaaac atttttcaaa gagcaatatg gtaagtggat cgaatgctgg agaaattgtt
     3481 tggcaaaaag atgaaatcgt gttgttcttg gatgatcatg atcaaattag gagaaatttt
     3541 gataaagctc gatttcccgc aggttctgat gagtggagta gattagcatt ttcggcatta
     3601 gcgaccttgg tatgtagtgc ggggattcca tgcatctact atggtagtga gcagggtttt
     3661 gacggccaag gtcgccacga tcggtatatt cgagaaacca tgtttgccag cgaatttggc
     3721 gcctttcgtt ctaggggttg tcactgtttt aatgaacggc atgcaattta tcaacagctt
     3781 caacggttgc tcgccatccg taagcatttg cctgctctga ggcagggaga ccagcaacta
     3841 ctagaaattt ctggagatgg cgagcacttt gg
//
LOCUS       DAOQZP010000064         3868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_24198_length_3868_cov_5.509310, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000064 DAOQZP010000000
VERSION     DAOQZP010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24198_length_3868_cov_5.509310"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..964)
                     /locus_tag="PKH60_04445"
     CDS             complement(32..964)
                     /locus_tag="PKH60_04445"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF000824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HNQ17322.1"
                     /translation="MPKTNPSDSNKVAIVGCGRVGMSAAFSILHSGVVNELVLYGRHK
                     AEIIGEELDLEHGIAFLHQATIHATESYEDLADSDVVIVTAGASQKPGDTRLDLAAKN
                     VSIMESIIPEIVKVAPNAVIIMVSNPVDVLTYKAYQMAGLPKGQIFGSGTVLDTSRFR
                     FHLSEFLKVNPRSIHAYILGEHGDSSFPVISQATVGGQPLNEFPNFSDKKALEAYEKA
                     RKAAYTIIESKGATYYGIAAVLSHIVKCVLTDSKSVLPVSIPLHNFHGHSGVALSVPC
                     VVGRRGVETILQPKLSWEEKQDLEKSVNTLKKYL"
     gene            complement(1006..1950)
                     /locus_tag="PKH60_04450"
     CDS             complement(1006..1950)
                     /locus_tag="PKH60_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNQ17323.1"
                     /translation="MVTMLATRDGVGRALLELGKTNPQVVVLTADLAESTKVEEFAKK
                     FPQRFIEVGVAEQNMAGTAAGLAIGKKIPFITSYGVFSPGLNWGVIRSAIAYSNLPVK
                     IIGGHSGLATGPDGATHQALEDIALMRALPNMIVLAPADEEEAFQATLAAAQHSGPVY
                     IRTSKLPVERVTTEKKPFEMGRAQVLKQGSDVTIIAHGTMVARAITASKLLEHIAISA
                     RIINSPSIKPLDKTTILQAFRQTQAVVVVEDHQVAGGLGSAIAELLSQETIRAPFKIL
                     GIQDSFGESGTPDELYQKHELTAEHITEVVSGLFHSST"
     gene            complement(1944..2789)
                     /locus_tag="PKH60_04455"
     CDS             complement(1944..2789)
                     /locus_tag="PKH60_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNQ17324.1"
                     /translation="MEANEDQQLQTIAQAIRQSVVLMLLESGSGHSAGSLGMADVFTA
                     LYFGNVVRHDPTQPDWPERDRVMLSNGHICPVLYATLAEANYFSKEELHSLRKFNSRL
                     QGHPHLGSLPGIENTSGPLGQGLSQALGLALAFKMDTLPNRVYCLLSDGEHQEGQTWE
                     AYQFGAAKKLDNVTALIDRNNIQIDGFTEQVMPIEPLEEKITSFGWQVFEVDGHNISA
                     VIEACNHARAIHDRPTAIICNTIPGKGVEFMENLPEWHGKTPTLPDTIEAVQQIRSLQ
                     NRIQW"
     gene            2828..3577
                     /gene="truB"
                     /locus_tag="PKH60_04460"
     CDS             2828..3577
                     /gene="truB"
                     /locus_tag="PKH60_04460"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNQ17325.1"
                     /translation="MSDEFTFSGFEEIQSLKKGMLLVDKPVGPTSHDVVAWARKIFQV
                     RKIGHTGTLDPLASGLLILLISRDFTKLQTQLLKQDKTYLVTAELGITSGSYDCTGTI
                     SQTNVVVQPTEAQIGVALDQFIGEIDQEVPAFSAVKVGGKKLYKIARRNSTILPQLPS
                     RKVVISNIELIEYAWPNVTLRIDCSSGTYVRSLINDLGKVLNVGAMVTSLRRERIGTV
                     TIEQTSVCPYIISRYRSPLTIETNRDDIKQQ"
     gene            complement(3513..3827)
                     /locus_tag="PKH60_04465"
     CDS             complement(3513..3827)
                     /locus_tag="PKH60_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17326.1"
                     /translation="MTKKISPPKTLDAAFDLVSAELLEMFLKKHRDYGKGNILANKEL
                     GIAMRVSEKVERLKHLLMTGNEPTNEQIEETWVDIGVYSIIAVLYRRGWFQSLEVNDN
                     AK"
BASE COUNT         1041 a          833 c          845 g         1149 t
ORIGIN      
        1 attgtcccag atgtcagaaa taattaaccg cttagagata ttttttaaga gtatttactg
       61 atttttccaa gtcttgtttt tcttcccagc ttaatttggg ttgcaaaatt gtctccactc
      121 ctcgcctgcc tacgacacaa ggaacactaa gcgcaactcc cgagtgaccg tgaaaattat
      181 ggagtgggat ggaaactggc agtactgatt tggaatcagt gagcacacac ttcacgatat
      241 gagacagcac cgcagcaatg ccgtaatacg ttgcgccttt tgattcaata atagtgtagg
      301 cggccttgcg ggctttttcg taagcttcaa gtgctttttt atcagaaaaa tttggaaatt
      361 cattcaaagg ctgacctcca acagtagctt gagaaatgac gggaaaagaa ctatcaccat
      421 gttctcccaa aatgtacgca tgaatgcttc tgggattaac ctttaaaaat tctgaaagat
      481 gaaaacgaaa tctagaagta tccagtaccg tgccagatcc aaaaatttga cccttgggca
      541 agcccgccat ttgataggct ttatacgtaa gtacgtcgac gggatttgat accataatta
      601 taacggcatt aggagcgact ttaacaattt ctggaatgat cgattccata attgatacat
      661 tttttgccgc caaatcgagg cgagtatctc ctggcttttg tgaagcaccg gccgttacga
      721 ttaccacatc gctatcggct aaatcttcat atgattcagt ggcatggatg gtggcttggt
      781 ggagaaatgc aataccatgt tctagatcaa gctcttcacc gatgatttct gctttgtggc
      841 gaccgtacaa gactagttca ttgacgactc cgctgtgtag gattgagaat gccgcgctca
      901 taccaacacg tccacaacca acaatggcaa ctttattgga atctgaggga tttgttttgg
      961 gcatactttt actatacaga aaaaaataat gaagttcgac tagaattagg ttgatgaatg
     1021 aaagaggcca ctcaccactt cggtaatatg ttcagcagta agttcgtgtt tttgatacaa
     1081 ctcatctggt gtgcctgatt cgccaaaact atcttggatt ccaagaattt taaaaggtgc
     1141 tcggattgtt tcctgtgaga gcaactcggc aatggcggag ccaaggcctc cggctacttg
     1201 gtgatcctca accactacta cagcctgtgt ttgacggaaa gcctgtagga ttgtcgtttt
     1261 atcaagaggt ttaattgatg gtgagttgat aatccgtgct gatattgcga tgtgttcaag
     1321 caacttgctc gctgtaattg cgcgcgcgac catggtgccg tgagcaatta ttgtgacgtc
     1381 tgagccttgc ttgagcactt gcgcgcgccc catttcgaat ggttttttct cagttgttac
     1441 tctttcgacg ggcagtttac tggtacgaat atacacaggc ccagagtgtt gagccgccgc
     1501 taaagttgct tgaaatgcct cttcctcatc ggcgggagct agcacgatca tattggggag
     1561 cgctcgcatc agtgcaatat cttcaagagc ttgatgagtg gcgccatctg gtccggttgc
     1621 taaccccgaa tgtccgccaa taatttttac cggtaaattg ctatacgcaa tggcgctccg
     1681 aatcactccc caatttaatc cggggctaaa aactccgtag ctcgtaatga agggtatttt
     1741 tttgccgatt gctaaaccag cggcagtacc agccatgttt tgttcggcta cacctacttc
     1801 aatgaatcgt tgtggaaatt ttttcgcaaa ttcttcaact tttgttgatt cggctaaatc
     1861 agcagttaaa actacaacct gaggattggt tttgcccaac tcaagtaaag ctctgcctac
     1921 cccgtcacgt gttgctaaca tagttaccat tgaattcgat tttgcaaact tctaatttgt
     1981 tgtacagctt caattgtatc cggaagtgtt ggagttttac catgccattc tggcaaattt
     2041 tccataaact ctactccttt accagggatg gtattacaaa taatggcggt tggtcgatca
     2101 tgaatggctc tcgcgtgatt gcaggcttca atcactgcag aaatattatg tccgtcaacc
     2161 tcaaaaacct gccagccaaa ggaagtaatt ttttcttcta gtggctcgat cggcataact
     2221 tgttcagtaa aaccatcaat ctgtatatta tttctgtcta tgagcgctgt cacattatct
     2281 aatttttttg cagcgccaaa ttggtaggct tcccaagttt gaccctcttg gtgttcacca
     2341 tcacttagta ggcagtacac acgattaggc aaagtatcca ttttgaaggc tagcgctaat
     2401 ccaagtgctt gcgacaatcc ctgaccaagt ggcccagatg tattttcaat tccaggcaga
     2461 ctaccaaggt gtggatggcc ttgtagtcga ctgttaaatt ttcggagtga atggagttct
     2521 tctttggaga aatagtttgc ttctgctaat gtggcataca aaactgggca aatgtggcca
     2581 tttgaaagca tcactcgatc ccgttcaggc cagtctggtt gggtgggatc gtgtctgacg
     2641 acgttgccaa aatatagtgc ggtgaaaacg tccgccatcc cgagtgagcc cgcgctgtga
     2701 cctgagccag attcaagcaa catcaacaca actgactgtc ggatggcttg agcaattgtt
     2761 tgcaattgtt ggtcttcatt tgcttccaca actcaagcat agcaaattac gagtacaatg
     2821 ggtagccatg agcgacgaat ttactttttc tggatttgaa gaaatccaaa gtctaaaaaa
     2881 aggaatgttg ctggtggata agccagtagg tccgaccagt cacgatgtag tggcttgggc
     2941 aagaaaaata tttcaagtga gaaaaattgg tcacactggc acgctggatc cattggcgag
     3001 tggtttatta atattgttga ttagtcgtga tttcaccaag ctacaaactc agctattgaa
     3061 acaggataaa acatatctgg taactgctga attgggtatt accagcggca gttatgattg
     3121 cacgggtaca atttcccaaa caaatgttgt ggttcaacca accgaagctc aaattggggt
     3181 tgcgctggat caatttatag gtgaaattga tcaagaggtc cccgcttttt ctgcagtcaa
     3241 agttgggggg aaaaaactat ataaaattgc tcgtcgtaat tcgacaattt tgcctcagtt
     3301 gcccagtcga aaagtggtga tttcaaacat agagctcatt gagtatgcat ggccaaacgt
     3361 gacgctacga atcgattgta gctctggaac ctatgtgcgc tcgcttataa atgacttggg
     3421 taaagtactt aatgttggtg ccatggtgac ttcactccgt cgcgagcgca tcggcacagt
     3481 aaccatcgaa caaacctctg tttgtcccta tattatttcg cgttatcgtt cacctctaac
     3541 gattgaaacc aaccgcgacg atataaaaca gcaatgatgc tataaacgcc gatatccacc
     3601 caggtttctt cgatctgttc attggttggt tcattgccag tcatcagcag atgtttgagt
     3661 cgttcaactt tttcacttac tctcattgcg atccccaatt ctttatttgc gaggatgttt
     3721 cccttgccgt aatctctgtg ctttttgaga aacatttcta acaattcagc agaaactaag
     3781 tcaaaagccg catctaaagt ttttggaggt gagatttttt ttgtcatgtt tactatcata
     3841 acctatcatt agtcatctac aaataatc
//
LOCUS       DAOQZP010000065         3828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_24571_length_3828_cov_4.199311, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000065 DAOQZP010000000
VERSION     DAOQZP010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24571_length_3828_cov_4.199311"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PKH60_04470"
     CDS             complement(<1..234)
                     /locus_tag="PKH60_04470"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HNQ17327.1"
                     /translation="MKTTKTVIITTAQKLTDKQRSILKELIAQKIGTAIAIDEVVDAA
                     VIGGIRIQLGNQEFDATISGTLEKVDSLLEVIEV"
     gene            complement(238..723)
                     /locus_tag="PKH60_04475"
     CDS             complement(238..723)
                     /locus_tag="PKH60_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HNQ17328.1"
                     /translation="MDILIPQIIFQVINFLVVLGLLTYLLYKPILQIFKERSERIEKG
                     QKAAQEAINQQEKLVEYEEKKQRELEKKIATKLEKASKEAEEHKARLMEKAKVDVDDF
                     IDKQHKKTEQEKAQVLAKMQEGLADAVVLATEKILAKKLTKQEKDTLVTQQLEEALQG
                     M"
     gene            complement(776..991)
                     /gene="atpE"
                     /locus_tag="PKH60_04480"
     CDS             complement(776..991)
                     /gene="atpE"
                     /locus_tag="PKH60_04480"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNQ17329.1"
                     /translation="MSKEFAIAFVMSVGAIGPAIGIGLIGMSGLSAIGRNPEAASKIQ
                     TNMILAIAFAEAVAIYALVVALILKFV"
     gene            complement(1049..1948)
                     /gene="atpB"
                     /locus_tag="PKH60_04485"
     CDS             complement(1049..1948)
                     /gene="atpB"
                     /locus_tag="PKH60_04485"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNQ17330.1"
                     /translation="MTGIHVSISAETIFHLGSIPVTNSMFASLIISAFLIGFAVITRL
                     KLQNTDKPTGIQNVAEWLVETLHNLVFSVTGDIKKSARFFGPVATFFIFILLNNWFGL
                     LPGFGTIGFTHTPEPKEIVVEENALVVPAEEIIEPEIVAEDHSVKTPENLTEVEATET
                     AEAEESHDKGVFVPYLRAGTADLNTTIALALISFTLIQIFGVQYSQLGYFKKFFNFSS
                     PIDFFVGVLELISEIGKIISFAFRLFGNVFAGEVLLVVISALVPILIPMPFYGLEIFV
                     GLVQALVFAMLSLVFYNIATQHH"
     gene            complement(2050..3801)
                     /locus_tag="PKH60_04490"
     CDS             complement(2050..3801)
                     /locus_tag="PKH60_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17331.1"
                     /translation="MLNTQKLLYILPDVAYVAELLPGKKPHSFSIQSFRQINGEYLDE
                     NTFIAENVIKLLSKLEDDNYHLVLPDSLFTNTIVTVKETEDKKIEAFINENLLPQLEI
                     NRETHQIAISKLTTLKGESRVQLSALEQSLLAPLRVGSGKTKNKIVAVSPLSWTLKSV
                     VSLEPSITILQMGKNLFSALHYIGVDQAFETIVDDVEAIAETIKTLKGSEPNIQTVYL
                     VTNAVVEEKLKDLLSDTIPLQQLNLLKEDDAKVPSYVKYCIEAGQKTISISDYPVPKF
                     TLGKSTSEDESIQVEKLTEDKKPLEIEAADELPTLPEPNLPTISATVEDQQENDSMPA
                     TKPKSTSENETLIESKPEVVEESKSDAAPIVMDNASSIDSDTSTPSTGDVDLSQFAVH
                     TTDDVVTPVVSEEKPTVVSATKPIKNQSGVGSMIKMIVITLVVFIATVAVGVGIGFGL
                     LTLSKSKTPVTPTPTEVTEVAPTATPEPTPVASPSAVLNPEDASLLVVNATTKAGYAS
                     TIKDMLTKAKFGSITASNAKGEYATAENNLVLMAEENPALLQQLEKATNLGLAFAEGY
                     STEDAKGTYDAVIVLTK"
BASE COUNT         1046 a          738 c          771 g         1273 t
ORIGIN      
        1 aacctcaatt acttccagca aggaatctac tttttctagt gttccggaaa tagtcgcgtc
       61 aaattcttga ttgcccaact gaatccgaat accaccgatt actgcagcat caaccacttc
      121 atcaattgca attgctgtac caattttttg agcgatcaac tcttttaaaa tggatcgctg
      181 cttgtcagtc agtttttgag cagtagtaat aatgactgtt ttagttgttt tcattgatta
      241 cattccttgc agcgcttctt cgagttgttg agttacgagc gtatcttttt cttgcttggt
      301 taattttttt gcaagaattt tttcggtggc tagcacaact gcatccgcta atccttcctg
      361 cattttagca agtacttggg ccttttcttg ctcggttttt ttatgttgtt tgtcaatgaa
      421 atcatcaaca tcaactttgg ctttttccat taatcgtgcc ttgtgttctt cagcttcttt
      481 tgaagctttt tctaactttg tcgctatttt cttctcaagt tcgcgttgtt tcttttcttc
      541 gtattcaacc agtttttctt gttgattgat agcttcttga gctgcttttt gacccttctc
      601 aattcgctcc gatctctctt taaaaatttg tagtattggt ttgtaaagta aatatgtgag
      661 caacccaagt actactagaa agttaatcac ctgaaagatg atctgaggta tcagaatatc
      721 catacgactc cttatcttca atatactatt tagctcgggt caccctgctg attgattaga
      781 cgaactttaa aatcaatgca accacgagcg cataaattgc gactgcttca gcaaaggcaa
      841 tggccaagat catgttggtt tgaatcttac tggcggcttc tgggtttcta ccaatagcac
      901 tcaggccact catgccaatt agtccaatac cgattgctgg accaattgca ccaacgctca
      961 tgacgaaagc aattgcaaat tcttttgaca ttttttcctc ctctaatacg tttatatttg
     1021 tttttactac gatgttaccg attaacgatt aatgatgctg tgtggcgatg ttgtaaaaga
     1081 ccaaggacaa catcgcaaat acgagcgctt gaaccagacc aacaaaaatt tccaagccat
     1141 aaaatggcat tggaattaag attggaacta gggctgatat gacgactaat aaaacttcac
     1201 cagcaaaaac atttccaaat aaacgaaatg caaatgagat tattttgcca atttcggata
     1261 taagttctag aactcccaca aagaagtcaa tggggcttga aaaattgaaa aactttttga
     1321 agtaacctaa ctgtgaatat tgaacgccga atatctgaat tagggtaaat gaaattaatg
     1381 cgagagcgat ggtggtattg agatctgctg tgccggctct aagatagggt acgaaaacac
     1441 ccttgtcgtg gctttcttca gcttctgcag tttctgtggc ttcaacttct gtaagatttt
     1501 ctggtgtttt aaccgaatga tcttctgcaa ctatttccgg ctcaatgatt tcttctgcag
     1561 gtactacaag agcgttttct tcaactacta tttcctttgg ctcaggagta tgagtgaatc
     1621 caatggtgcc gaagcctggc aacaaaccaa accagttatt tagcaagata aatataaaaa
     1681 aagtggctac aggaccgaaa aaacgagcgc ttttttttat atctccagtc acggaaaaca
     1741 ctaaattatg caacgtttct accagccact ctgcaacatt ttggatacca gttggtttgt
     1801 ctgtattttg aagctttaat ctggtaatca cggcaaaacc gatgagaaaa gcagaaataa
     1861 ttaaacttgc gaacattgag ttggtgacag gaatagaacc taggtggaag atggtttccg
     1921 cgctgatcga aacgtgtatt cctgtcataa agtatggttt ctctttttca aaacaaaaaa
     1981 acgtcctagc tccttctatc atacaattga tggaggagct gaaacgctag ctgattatac
     2041 ccaagctatt tatttagtca acactattac ggcgtcgtac gtgccttttg cgtcttctgt
     2101 gctatagcct tcagcaaagg ctaggcctaa attggtagct ttctctagtt gttgtagaag
     2161 agctgggttt tcttcagcca tcagcactaa attgttttca gcagtggcat attcaccttt
     2221 ggcgttacta gcggtaatag atccaaactt agccttagtt aacatatctt tgattgtgct
     2281 cgcatatcca gcttttgtgg tagcgttaac cacgagtaga cttgcgtctt ctggattaag
     2341 gacagcagat ggcgatgcca ctggggtggg ttctggagtg gcagttggag caacctcagt
     2401 aacttcggtg ggggtgggag taactggagt ttttgattta gacaaagtca gcaatccaaa
     2461 tcctatacca acaccaacag ctacggtggc gatgaagact actaaggtga taacaatcat
     2521 ttttatcata gatccaactc cagattgatt tttaattggt ttggtggcag atactacggt
     2581 tggtttttcc tccgaaacta ctggagtgac cacatcatct gtagtatgaa cagcaaactg
     2641 agataagtct acatcacccg tgctcggagt ggaagtatca gaatcgatcg aactagcatt
     2701 gtccatgaca attggagcag catcagattt tgattcttca acgacctcgg gctttgattc
     2761 tattaaagtt tcattttcac tggttgattt gggttttgtt gctggcattg agtcattctc
     2821 ctgttgatct tctactgtgg cggaaattgt tggtaggttt ggttctggta gcgttggtag
     2881 ctcatcagcc gcttcaattt ctagtggttt tttatcctca gtcaattttt caacttgaat
     2941 gctttcgtct tcagatgtgg attttccgag agtaaacttt ggtaccggat aatcactaat
     3001 agaaatggtt ttttgccctg cctcaatgca gtacttcacg tatgatggaa cttttgcatc
     3061 gtcctctttg agcaagttaa gttgttgtag aggtatagtg tcgctgagta ggtcttttaa
     3121 tttttcttca acaactgcat tagttacgag ataaaccgtt tgaatatttg gctcactgcc
     3181 ttttaacgtt ttgatagttt cagcaatcgc ttcaacatca tcgacgatag tttcaaatgc
     3241 ttggtcaact ccaatataat gaagggcgct gaagagattt tttcccattt gtaatatggt
     3301 gatggatggt tcgagcgaaa caactgattt taatgtccag ctcaaaggag aaactgcgac
     3361 aattttattt tttgttttac ccgaaccaac tctgagtggg gcgagtaatg attgttcgag
     3421 ggctgagagt tgaactcttg actcaccctt aagagtggta agtttactga tggcaatttg
     3481 atgggtttca cgatttattt caagttgtgg aagaaggttt tcattaataa atgcttcaat
     3541 ttttttatct tcagtttctt tgacagttac tatagtgtta gtgaaaagac tgtctggtaa
     3601 taccaagtga tagttatcat cctccaattt tgagagcaac ttgataacat tttcagcaat
     3661 aaatgtattt tcatctagat actcaccatt gatttgtctg aaagattgaa tagaaaatga
     3721 atggggtttt ttgccaggaa gcaattcagc cacataggcc acgtcaggga gtatatagag
     3781 cagtttctga gtattcagca tccttgtcca ctagtatgaa aaaaaact
//
LOCUS       DAOQZP010000066         3716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_25682_length_3716_cov_4.214969, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000066 DAOQZP010000000
VERSION     DAOQZP010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25682_length_3716_cov_4.214969"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="PKH60_04495"
     CDS             complement(<1..1013)
                     /locus_tag="PKH60_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17332.1"
                     /translation="MKKENKEQIVVSAANGKLRKPLEQLVLLAGFEISATDERSYETT
                     ISSPELEKNIQLVFAKPPDVSRLLSKNPKQITAGITGGDVLYEQRIEPDLLLDFGLIT
                     PWLAPVVLKLLATPNGLKKPEIQRLAELGRYESSSDTDPTSNQYQKMRATMQQLLVDS
                     TVYTSLTRMVSEYFENDQAITIFGLAGTIEGVWRSDSDSLVIADRVETGLTAQKNGLL
                     SIAPITELNKLGVLLSPNSSDEDLATVTQLSSKILKIPDQFGATKPWKRKPLTVEEII
                     TNGSLRWLYVLGQNNTRDDRFTPRPLFFDESMKTIDLAKKILGAEGTLVTSDDPARIR
                     QFF"
     gene            complement(1266..1601)
                     /locus_tag="PKH60_04500"
     CDS             complement(1266..1601)
                     /locus_tag="PKH60_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HNQ17333.1"
                     /translation="MTDTSSTNQKTIIELAKVLTRLENPSKMLNFLYDVATPKELEEL
                     AQRFQIAKLLWTTNLSYVEIAGQTNASTTTVTRVARFLNQEPFGGYRQVLNTFYPRAE
                     TKNPRRRKT"
     gene            1670..1745
                     /locus_tag="PKH60_04505"
     tRNA            1670..1745
                     /locus_tag="PKH60_04505"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1703..1705,aa:Ala,seq:cgc)
     gene            1811..2185
                     /locus_tag="PKH60_04510"
     CDS             1811..2185
                     /locus_tag="PKH60_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17334.1"
                     /translation="MILANEIQQPAIYENGSPDPIAEIIVESVCELKQGTSVLPLLDM
                     VKKKPEYSFSTKLLMVLPGETEAFEVTLEYQPYLLNDRICTFFLSGKRPGKSSIDGYI
                     AVAMKKLLKFPEGSIVEFDLVG"
     gene            2195..2353
                     /locus_tag="PKH60_04515"
     CDS             2195..2353
                     /locus_tag="PKH60_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17335.1"
                     /translation="MITIREYQDADTETIVDWFNQLHDYLVELDPIKRLRRAPEYGEV
                     FFESNLSN"
     gene            2733..3407
                     /locus_tag="PKH60_04520"
     CDS             2733..3407
                     /locus_tag="PKH60_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17336.1"
                     /translation="MGRKLSEQEGIMEMQLTPVEYILSSKVEYVTNDQSGLSTWQKLL
                     GKIRQADRVRVDWDGQSKEGLFQLGMAFALRKPIMIDSLPTFDDQTKSFPRMITAWAE
                     QPEFESGSIPDLQKKIFLICPVRNQDDVTRELLEELAQDLQVYYPAKETNQLDETGGL
                     RICADNRFAMESAREVWFVWDGLSQGSIFDLGMAFALAKPIKPIKLPPATSEPSFQNF
                     ARKYST"
     gene            complement(3434..>3716)
                     /locus_tag="PKH60_04525"
     CDS             complement(3434..>3716)
                     /locus_tag="PKH60_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012856094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNQ17337.1"
                     /translation="AYQKAVELVERRPTVLTASQIDAVTRGVIEKAGYGSQFIHTTGH
                     GLGLDIHEPPSLNSRNQQLIEKNMAITIEPGIYLPGKFGYRHEDTIQIT"
BASE COUNT         1051 a          731 c          816 g         1118 t
ORIGIN      
        1 tcaaaaaatt ggcgaattct ggctggatca tctgaagtaa ctaacgttcc ctccgcacct
       61 aagatttttt ttgctagatc aatagttttc atactttcat caaagaaaag aggccttggt
      121 gtaaatcgat catctcgagt attattttga cctaatacat agagccatct caaacttcca
      181 ttagttataa tttcctcaac agtcaatggt ttacgcttcc aaggtttagt tgcgccaaat
      241 tgatcgggaa tcttgagaat cttgctactc aactgcgtaa ccgtggcaag gtcttcatcg
      301 ctactgtttg gactcagtag cacaccaagc ttattgagtt cagtaatagg agcaatactt
      361 agtaaaccat ttttctgagc cgtgagacca gtttctactc ggtcggcaat cactaaacta
      421 tctgagtcac ttctccagac accttcaatt gtgcctgcta ggccgaagat ggtaattgct
      481 tgatcatttt caaagtactc agataccatt ctagtgagac tcgtataaac agtagaatca
      541 acgagtaatt gttgcatagt ggctctcatc ttttgatact ggttggaagt tggatcagta
      601 tcagagctgg attcatatcg accaagttct gccaaccgtt gaatttctgg ttttttcaga
      661 ccattcggag tagctaacaa cttaagcact accggcgcta accaaggtgt aatcaaacca
      721 aaatctagta acagatctgg ctcaattctt tgctcataca gtacatcacc cccagttatt
      781 cccgcagtaa tttgtttagg atttttacta agtaatctgg acacatcagg gggttttgca
      841 aacacaagct gaatgttttt ctccagttct ggcgatgaga ttgtcgtctc gtatgatcgt
      901 tcatcggttg cactgatttc aaagcccgcc aatagaacca gttgttccag tggttttctt
      961 aattttccgt ttgccgcact tacgactatt tgttctttat tttctttttt catatgttct
     1021 ttcttttgat tgcttactaa ggaatacaaa aactcccccg tcatttgtga atggcggggg
     1081 agtgaattac ttttctttct gattttttat actacgaaaa caactctccg ccattggctg
     1141 gggagtggtg atgaactact tggttgttac tatttttcat taacacgaga gagagtataa
     1201 caaaggtatc caaatatgtc aacatgctaa cacgataaca taaagaattc atttagatct
     1261 ggagcttagg tttttcttcg tctaggattt tttgtttctg cccttggata aaatgtgttc
     1321 aacacctgtc tataaccgcc aaaaggctcc tgattgagga aacgagctac tcgcgtaaca
     1381 gtggtagtac tggcatttgt ttgacccgca atttccacgt aagacaagtt agtagtccat
     1441 aatagtttag ctatttgaaa ccgttgagcc aattcttcta attctttggg agtggccaca
     1501 tcatacagaa agttgagcat ttttgaaggg ttttcaagcc ttgtcaggac tttagctaat
     1561 tcgataattg ttttttgatt agtagatgaa gtatcagtca tagtattact agtataactt
     1621 gaacagcatt ggcccgttag atgtataatc ggtggcacgc caaaaaaacg ggccagtagc
     1681 tcacttggta gagcgctgca ttcgcattgc agaggtaagg ggttcgattc ccctctggtc
     1741 caccattggt aactattcag aaatttgata ttcctgaccg tatattacgt aaactgaagg
     1801 tgtttttatt atgattttag ctaacgagat tcaacaaccg gccatatatg aaaatggttc
     1861 cccagatcct atagctgaaa ttatcgtaga gtctgtgtgt gaattgaaac aaggaacatc
     1921 tgtactgcct ttgctcgata tggtaaagaa aaaacccgag tactcattct ccaccaaact
     1981 actaatggtt ttaccaggag aaacagaagc ttttgaagtg actctagaat atcagccata
     2041 tctgctcaac gataggatat gcactttttt tctcagcgga aagagaccgg gcaaaagtag
     2101 tatcgatggt tatattgcag ttgcgatgaa aaaattgcta aagtttccag aaggatcaat
     2161 tgtcgaattt gatctggtcg ggtagactta atgtatgatt accattcgcg aataccaaga
     2221 cgctgataca gagaccatcg tcgattggtt taaccaacta catgattatc tagttgaact
     2281 tgatccgatc aagcgactca gaagagcgcc tgaatatggt gaagtttttt ttgagtctaa
     2341 tctcagcaac tagttgaagg aaatggtaag attttcattg ctagtgactc gggtgttgct
     2401 atcggttttg tcagtggttt tattgaacag caatctgaaa aaaatctgct ggagttggtg
     2461 ccaaccaaac ttggggttgt tgcagatctg tttgtggtgg aatcacaccg taaacagggc
     2521 gtaggggaag ctttgctgga acacatagag aactacttac aagatcgggg ttgtgatagt
     2581 ttgtgggtca atgtggtcgc tttcaaccca gctatcgatt tttataaaag taagggatat
     2641 ggggaacggg aagttggact gataaaaaga ataaagtgaa cactggtgtg atagactaat
     2701 cgagtctgct cagactaaca gaatgtgttg ctatgggtag aaaactttca gaacaagaag
     2761 gtattatgga aatgcaactg acgccagtag aatacatact ttcttccaag gtggagtatg
     2821 taactaacga ccagagcggc ttgagcactt ggcagaagtt gcttggaaaa atacgccagg
     2881 ctgatagagt ccgagtagat tgggacggac agtctaagga aggattattt caacttggta
     2941 tggctttcgc tttgcgaaag cccatcatga tcgacagctt gcccactttt gatgatcaaa
     3001 ccaaatcatt tcctcgaatg atcactgcgt gggcagaaca accggagttt gaatcaggct
     3061 ccataccaga tcttcaaaag aagatctttc tcatttgtcc ggttcgaaac caagatgatg
     3121 tcactcgaga attactagaa gaattggccc aagatcttca agtgtactat ccagcaaagg
     3181 aaaccaacca acttgatgaa actggggggt tacgaatatg tgctgacaat cgatttgcga
     3241 tggagtcagc acgagaggtc tggtttgttt gggacggcct ttctcaagga agcatttttg
     3301 atctaggtat ggcatttgcg ctggcaaaac cgattaagcc gatcaagcta ccgccagcaa
     3361 ccagcgaacc ctcatttcag aattttgccc gaaagtattc aacttgatca aagtcagttt
     3421 tcaacaaaat gatttaggta atctgaatcg tatcctcatg cctatatcca aacttgcctg
     3481 gcagatagat accaggctcg atggtgatag ccatgttttt ttcgatgagt tgttggttgc
     3541 gactgttgag tgagggcggc tcgtggatgt cgagtcctag gccgtggccg gtggtgtgaa
     3601 tgaactgcga gccgtagccg gctttttcta tcacgccacg agtcactgca tcaatctgcg
     3661 aagcagtaag aactgtaggt ctacgctcca caagttcaac agctttttga tatgca
//
LOCUS       DAOQZP010000067         3660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_26252_length_3660_cov_7.678779, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000067 DAOQZP010000000
VERSION     DAOQZP010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26252_length_3660_cov_7.678779"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..531
                     /locus_tag="PKH60_04530"
     CDS             238..531
                     /locus_tag="PKH60_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNQ17338.1"
                     /translation="MRQYWVYILRCSDNSYYTGVTNNLDRRVNEHNLGVHQSCFTFRR
                     RPVKVVYFSKFSWIQKAIAFEKQLKGWSRAKKEALIEGEFEKLPGLSASMNSA"
     gene            complement(592..948)
                     /locus_tag="PKH60_04535"
     CDS             complement(592..948)
                     /locus_tag="PKH60_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17339.1"
                     /translation="MKLFRYVFVVLILGVVGLVIGYKTNTLPSQVKQAVDSVATRLPK
                     TQVAIDQALLASQAAQLTERGSQVLEQGTKVLGAAVTVDESQENSSLTDRALNYGRYL
                     YCKQVVEDWETRTSAP"
     gene            complement(964..2181)
                     /locus_tag="PKH60_04540"
     CDS             complement(964..2181)
                     /locus_tag="PKH60_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNQ17340.1"
                     /translation="MFVATLILAIQSIFRNKVRSFLTMLGIIIGVGSVVLLTSIGNGL
                     QAYVSEQFEALGSNTIYVVPGTPFGEGGGFGGNQEQTFLETTKTTLKSSYLKQILRNQ
                     RSLISDGASTNLTIAKAKYLKTEKKVTLLGVSASYAEIQSADTSKGEWFSEVDEKKAS
                     PVLLLGSKIATELFGEIDPVGKRIQLDSQTYTVLGVLKTRGGGFGGPSFDNYVYLPFN
                     TVVERFDVNYIDNFVLTVRDASQIAEAKNQIKSELLQHLKEDEFTVFDQTQLLDTINS
                     ILGVLTAGLGGIAAISLVVGGIGIMNIMLVSVTERTREIGLRKALGATPNIILIQFLI
                     EAALLSAFGGLIGLAIAYGGSLLIQQFFPAKVTLDAVMLAVGVSTVVGLIFGAAPARR
                     AAQLSPIEALRYE"
     gene            complement(2185..2925)
                     /locus_tag="PKH60_04545"
     CDS             complement(2185..2925)
                     /locus_tag="PKH60_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNQ17341.1"
                     /translation="MSRNTSQPLLELKNVSRRYEVGDTIIKALDGVSITVQTGEFIAV
                     MGPSGSGKSTFLQIASMLATPSAGAIFLKGKDVTAYNEIERARLRNKEIGFVFQQFNL
                     LPRTSALDNVKLPLVYARVTEKDQSERAKKMLEIVGLADRMKNTPAQLSGGQQQRVAI
                     ARALINDPSIIFADEPTGNLDTKSGHDIIALLQDLHRNGKTIIMVTHEEDIAKAAKRQ
                     IRFVDGKIVHDTQTDVSISSKSTKKGKR"
     gene            complement(2939..>3660)
                     /locus_tag="PKH60_04550"
     CDS             complement(2939..>3660)
                     /locus_tag="PKH60_04550"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNQ17342.1"
                     /translation="NYLKERWDWEQTRDDKVENSDGTNRAQPELSIRRTVDQAQWDLE
                     NSVLTVEINDIAIQNTRMTSPIEGVLVSAPTSVTGVQLTAADAFVVVNPKTLVFKAAV
                     DEVDIASVKLAQSAEIELDAYQDKLIPSSVTYIAYSSNQTSTGTVFLVEFPITPDKGG
                     LDYFRLGMNGDVSIKLDEKANALVIPIQSTREKDGKTLIDIQTSEGKIEEREITTGME
                     TDEEIEVLTGLSETDQVVVPN"
BASE COUNT          979 a          812 c          785 g         1084 t
ORIGIN      
        1 gcttggtgca gtgatttcag tcactcgata agttcacaat caaaggtgaa agtcggccca
       61 gcccctcgcg aggggctggg ttttttttgc ttcgatgaac tcagcatgac attagtctgt
      121 caccttgagc tagtcgaaag gtgatcctgt gaagctgtca tcctgagttt gtcgaaggat
      181 agacagcgaa ctgaagactc tacgcttaac gtaaccaaat actgctaaaa acgtggcatg
      241 aggcaatatt gggtctacat tctaagatgt agcgacaatt cttactacac cggcgtcacc
      301 aacaatttag acaggagagt aaatgaacac aatcttggtg tacatcaaag ttgttttaca
      361 tttagaagaa gaccagtaaa agtagtttac ttttcaaaat tttcatggat ccaaaaggct
      421 atcgcttttg aaaaacagtt gaaaggttgg agtagagcta agaaagaggc tttgatagaa
      481 ggtgaatttg aaaagttgcc ggggttgtct gcttcgatga actcagcatg acaacccaac
      541 tagctcagtc tgacaacacc actgtcacac tgagcttgtc gaagtgtgac attaaggtgc
      601 cgaagtgcga gtttcccagt cttcaactac ctgtttgcaa tacagatagc gaccatagtt
      661 gagggctcta tcggtcaagg agctattttc ttgcgattca tcgacggtta ctgctgctcc
      721 aagtaccttc gtaccttgtt caagcacttg tgagcctctt tcagtcaatt gcgctgcttg
      781 gcttgccagg agtgcttgat ctatggccac ttgggttttt ggcaggcgag ttgctacaga
      841 atcaactgct tgtttcactt gtgagggaag agtgttcgtt ttatatccaa tgaccaaccc
      901 aacaactccc aaaatcaata caacaaacac atagcggaac aatttcatgt cattagtata
      961 ccgttattcg tagcgaagtg cttcgatggg tgagagttgc gccgctcttc tggcgggagc
     1021 ggcgccaaag atcaatccaa ccacagtaga aaccccaacg gccaacataa cggcatctag
     1081 cgttactttt gctgggaaaa actgttgtat gagcaatgag ccaccatatg caatcgccaa
     1141 accaatcaag ccaccaaatg ctgaaagtaa ggctgcttca atcaaaaact gaatcagaat
     1201 aatgtttggg gttgctccaa gcgcttttcg gagcccaatt tcccttgttc tttcggtaac
     1261 agacaccaac ataatattca taatgccaat accgcctacc accagtgaga tggctgcgat
     1321 gcctcccaaa ccagctgtaa gtacacctaa gatgctgttg atagtatcaa gcaactgagt
     1381 ctggtcaaaa acggtaaatt catcttcttt aagatgctga aggagttcag atttaatctg
     1441 attttttgct tctgcaattt gcgaggcatc tcggacggtt aaaacgaagt tgtcaatata
     1501 atttacatca aatctttcaa ccactgtatt aaacggtagg tacacataat tatcaaagct
     1561 tggaccaccg aaccctccgc ccctggtttt tagcactccc agcactgtat aagtttggct
     1621 atcaagctgg atacgtttac cgactggatc tatttcacca aataattcag ttgcaatttt
     1681 gcttccaagt agtaagacgg ggctagcctt cttttcatct acctcgctaa accactctcc
     1741 tttgctggta tctgcacttt gaatttcggc ataactagcc gagacgccca ggagagtaac
     1801 ttttttttcg gtctttaagt atttggcttt cgcaatggtc aggttggtcg aggcaccgtc
     1861 agaaataagc gatctttgat tacgcagtat ttgtttgaga tagctcgatt tgagggtggt
     1921 tttggtagtt tccaaaaaag tttgctcttg attgccacca aaaccaccac cttcaccaaa
     1981 gggtgtgcca ggcacaacat aaatagtgtt gctacccagg gcttcaaact gctcactgac
     2041 atacgcttgc aaaccattcc cgatgctggt cagtaacact acagatccca ccccaatgat
     2101 aatacccaac atggtcaaaa atgatctaac tttattgcgg aagatagatt gaattgccag
     2161 tatgagcgtt gcaacaaaca tagcttatcg tttgcctttt tttgttgatt tgctacttat
     2221 agaaacatca gtttgcgtgt cgtgaacaat tttgccatca acgaatctga tttgtcgctt
     2281 tgccgctttg gcaatatcct cttcatgcgt gaccatgatt atggttttac catttcgatg
     2341 cagatcttgg agtagggcaa tgatgtcgtg accactcttg gtatccaaat tgccggtggg
     2401 ctcatcggca aaaatgatac ttggatcatt aattaacgct ctggcaatgg ctacacgctg
     2461 ttgttgacca ccggaaagct gagccggagt gtttttcata cgatcagcta gcccaacaat
     2521 ctctagcatc ttttttgctc gctcactttg gtctttttcg gttacgcggg catacactag
     2581 tggtagtttt acattatcga gcgcgctggt tctgggaagt aggttaaact gctggaaaac
     2641 aaagccaatt tctttatttc tgaggcgcgc tcgctcaatc tcgttgtacg ccgtcacatc
     2701 tttgccctta agaaaaattg ctccagctga tggtgtggcc agcatactgg caatctgtaa
     2761 aaatgtagat ttaccgctgc cagagggtcc catgacggca ataaattctc cagtttggac
     2821 tgtaatacta acgccatcga gcgctttaat gatggtatca ccaacttcat agcgccgaga
     2881 aacatttttc agctcaagca gtggctgaga ggtattgcgg gacatagttg taattgctct
     2941 aatttggtac taccacctga tcagtttctg ataaacctgt gagtacctca atttcttcgt
     3001 ctgtttccat accagtggta atttcgcgtt cttcaatctt tccttctgag gtttggatat
     3061 ctatcagtgt ttttccatct ttctctctgg ttgattgaat gggaatgact aaagcatttg
     3121 ctttttcgtc gagtttaata gaaacatctc cattcatacc gaggcggaaa taatccaagc
     3181 cacctttgtc tggtgtaatg ggaaattcaa caagaaacac cgttccagtt gaggtttggt
     3241 tactagaata ggcaatatag gtgactgatg atggaatcag tttatcctga tacgcatcaa
     3301 gctcaatttc tgccgattga gcgagtttta cagaagcaat atcaacttcg tctaccgctg
     3361 ctttaaagac gagagtcttg ggatttacca ctacgaatgc atcagcagct gttaattgaa
     3421 ctcctgtaac ggaagttgga gcggaaacaa gaacaccttc aatcggagat gtcattcttg
     3481 tattttggat ggcaatatcg ttaatttcta ctgtcagcac cgagttttcc agatcccact
     3541 gggcttgatc tacggttctt ctgatggata actctggttg ggcccgattg gtgccatcgc
     3601 tgttttcaac cttatcatct cgagtttgtt cccaatccca gcgttcttta aggtagttat
//
LOCUS       DAOQZP010000068         3504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_27919_length_3504_cov_5.723688, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000068 DAOQZP010000000
VERSION     DAOQZP010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27919_length_3504_cov_5.723688"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..688)
                     /locus_tag="PKH60_04555"
     CDS             complement(146..688)
                     /locus_tag="PKH60_04555"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNQ17343.1"
                     /translation="MKSSEVSYQSLKTSVSRAVLIASDVDPKSGQKYADEVLFTIRPG
                     WSKVQPYVAALPGGKIEADKKDFSGIVEVDSVENPWLVLTLEQMIQAGLKAAEREILE
                     ELGIVLTASLLQFVDVSTNTAGWTTYSYVGKLAEKPTVTVRPDSAGARWISVQQLLEG
                     KPKLLSGHLGIARRAIKKVR"
     gene            complement(773..1201)
                     /locus_tag="PKH60_04560"
     CDS             complement(773..1201)
                     /locus_tag="PKH60_04560"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNQ17344.1"
                     /translation="MSNFLRGFLFLISLLLFFTSFYLVYIQVSFLQHAKRASGTIVDY
                     VERCSGSDFDNCDSSSKIEFITDTGETISFVNRLSSKTTVESVTKQKTTPNTTVAVLY
                     DPQNPQDARIDSFMNKWFLPIFSLTTSVLLILFVTLTKKR"
     gene            complement(1272..1409)
                     /locus_tag="PKH60_04565"
     CDS             complement(1272..1409)
                     /locus_tag="PKH60_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17345.1"
                     /translation="MPVKKKKAAAKKVVKKTVKKKVAVKKVVKKAVKKPAAKKVAKKK
                     K"
     gene            1499..1687
                     /locus_tag="PKH60_04570"
     CDS             1499..1687
                     /locus_tag="PKH60_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17346.1"
                     /translation="MSSSRIVREFHLIDGDKVIKDLGLTSKAWNSIHDHLIDRALQVE
                     PGIHVVISQALIPLISGL"
     gene            1747..2115
                     /locus_tag="PKH60_04575"
     CDS             1747..2115
                     /locus_tag="PKH60_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17347.1"
                     /translation="MQDEIRRAGEGDVEPTEFDKGPVDLPDETPEQKIQSFTSLIQIY
                     DFVLKVKEEQLGGEVWNLLKTRALAICKEYAEILKTNPENLTAYRLELSNIGRCNLFD
                     TTDLENFARQWADAVTQKPE"
     gene            complement(2145..2312)
                     /locus_tag="PKH60_04580"
     CDS             complement(2145..2312)
                     /locus_tag="PKH60_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17348.1"
                     /translation="MLRLSVLLESGWSQLTHQVLWVKAKLSQFFWSVVFWYSTKKQQR
                     PHQVIEIEEEV"
     gene            complement(2341..2694)
                     /locus_tag="PKH60_04585"
     CDS             complement(2341..2694)
                     /locus_tag="PKH60_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17349.1"
                     /translation="MRRVLSHPAFMLLVGILAVLFVLSLRSTQKLTTQRGIQVEKNQL
                     EVESLKSKQEQLQKQLEESQLPFNQEKVLRNELRQQKEGDIVIQIPSIAPQISPPPSP
                     TPTITNWKVWMEIIF"
     gene            2847..>3504
                     /locus_tag="PKH60_04590"
     CDS             2847..>3504
                     /locus_tag="PKH60_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNQ17350.1"
                     /translation="MQKLLSEAQAQFVSSLQDAGKARATVLAYGKDVEQLVDFVATLG
                     KMYAGETTSEDIDAFKELLKKQRYTGKSISRKINSIKAFFRFMVTEKLIDTNPAGIIA
                     HPKFDQSPPRVLSKIEYRALRDAARGDARMCAVVEILLQTGMRISELAALQLTDVDFE
                     RNVVTIVPQNSRGGRKVPLNTAAKKALMEYLEVRPRAREKTFFLTKTCRPFLVRNIRT
                     A"
BASE COUNT         1083 a          677 c          685 g         1059 t
ORIGIN      
        1 cacgtgaggc tcataatctt acaggccggg ttcgactccc ggccccgcaa caatagcaaa
       61 atttaattaa acatccaact aaaatattct ttttttgttg tttaatttaa acagatttct
      121 aagtacaaat atatttagtg cgttttcaac gtactttttt gattgccctg cgagcaatgc
      181 ctaagtgacc tgatagtaat ttgggtttac cctcaagtaa ttgttgcacg ctgatccagc
      241 gcgctcctgc gctatctggt ctcacagtca ctgttggctt ttcagctaat ttcccaacat
      301 aactatatgt tgtccaacca gcagtattag tggatacatc aacaaactga agcaaacttg
      361 cggtcaaaac aattccaagt tcttccaaaa tttcacgctc tgcggctttt agacccgcct
      421 gtatcatttg ctccagagtt aaaactagcc acggattttc tacagagtca acttcaacaa
      481 ttccagaaaa atctttttta tcagcttcaa tttttccgcc tggaagtgca gccacatatg
      541 gttgcacctt tgaccaaccc ggtcgaatag tgaacagtac ctcgtcagca tatttttgac
      601 ctgatttagg atcaacgtcg gaagcaatca aaacagcccg tgaaactgaa gttttcagtg
      661 attgataact aacttctgat gatttcatga actggaatat atgcagaacg tataaatagt
      721 caagtctgat ttattgtaac ttaacaccgc gcgggttgtt tgatgtaaaa agctatcgtt
      781 tttttgtcag cgtaacaaac aagatcaata gcacgcttgt agttaaagaa aatatcggga
      841 gaaaccactt gttcataaat gagtctattc gagcatcttg aggattttga ggatcatata
      901 aaacagctac agttgtattt ggagtggtct tttgttttgt aactgattcg accgttgttt
      961 tggatgaaag acgattaaca aatgatattg tttcgccagt atcagtaata aattcaattt
     1021 ttgaagatga atcacaattg tcaaaatcac tgccactaca gcgttcaacg taatcaacta
     1081 tggttccgct tgctcgtttt gcatgttgca agaaagaaac ctggatatat accagataaa
     1141 acgacgtaaa gaacaacaat aaagaaatga gaaataaaaa gcctctgaga aaatttgaca
     1201 taatttagtt gttattcagc tattaaaaaa cggcgttttg tgaacgccgt tttttgagtt
     1261 tcataaagta gttacttttt cttcttagca actttctttg cagctggttt ctttaccgct
     1321 ttcttgacta ctttttttac cgctactttt ttcttaaccg tttttttgac taccttcttg
     1381 gcagccgctt tctttttttt gacaggcata ctattctcca tactaaccta aaaaaaccag
     1441 aggttaattt aggactttac tattaactca ttctaaagaa aaaagagcga tactgtcaat
     1501 gagtagcagt cgcatcgtac gtgaatttca tctcattgat ggcgataaag tcatcaagga
     1561 cctaggtttg accagcaagg cttggaacag tatccatgat catttgatag atcgggcact
     1621 tcaagttgaa ccaggcatac atgtggttat ttcgcaggcc ttgataccat tgattagcgg
     1681 tttatgatat gctagctcat gatttgatgt agaaaaaact ttttacaaat ctgatgaaag
     1741 gaacacatgc aagacgaaat taggcgggct ggagaaggag atgtggaacc cacagaattc
     1801 gacaaagggc cggtagacct gccagatgaa actcctgagc aaaagataca atcatttaca
     1861 tcattgatac agatatatga ctttgtcctt aaagttaaag aagagcaact cggaggtgag
     1921 gtttggaatc tattaaaaac acgtgctttg gcaatttgta aagaatatgc tgagatttta
     1981 aagaccaatc cagaaaactt aactgcatac cgacttgagc tttcaaatat aggtcgctgt
     2041 aatctttttg ataccacaga tcttgaaaat tttgctagac aatgggcaga cgctgttacg
     2101 cagaaaccag aataagtagt tacgctaatt agcgtaattg tgaatcaaac ctcctcttca
     2161 atctcgatta cttggtgcgg acgttgctgt ttcttagttg agtaccagaa caccacgctc
     2221 caaaaaaatt gagatagttt tgctttcacc caaagaactt gatgagttaa ttgactccaa
     2281 ccagattcaa gcagcactga caatcgcagc atggagattg gcaaaaaata aatattttcg
     2341 ttaaaaaatt atttccatcc aaactttcca gttggttata gttggagttg gagagggtgg
     2401 agggctaatt tgtggagcga tagaaggaat ttgtatcaca atatctccct ctttttgttg
     2461 acgcaattcg ttacgaagaa ccttttcttg attgaagggc aattgagact cctcaagttg
     2521 tttctgtaat tgctcctgtt tgcttttgag agactcaacc tctagttgat ttttttcaac
     2581 ctgaattcct ctttgtgttg tgagtttttg agtggatcgc aaagataaaa caaatagaac
     2641 tgccaatatt cctacgagca acataaacgc tgggtgagaa aggactctgc gcattgctat
     2701 ttaacctcca atatgatact ataagcctat tgttgagtgc ttgaatggta ttcaggctct
     2761 ataaagatcc atcatgacat gtttatagtc gtgatacaat agacgccagc ccaaagtaca
     2821 agacaagtaa gtagagacat tctagtatgc agaaactgtt aagtgaagca caagctcaat
     2881 tcgtaagtag tctccaggat gctggtaagg ctcgcgctac tgtgttggcc tatggaaagg
     2941 acgtagaaca actagtagat tttgtggcaa ctctaggcaa aatgtacgca ggtgaaacca
     3001 cttctgagga tatcgacgct tttaaagaac tactcaagaa acaaagatac accggtaagt
     3061 ctatttcgcg aaaaattaat tcgattaaag ccttctttcg ctttatggta actgaaaagc
     3121 tcattgacac gaatccagct ggcataattg cccatcccaa atttgatcaa tctcctccaa
     3181 gagtattgtc aaaaatcgag tatcgcgctc tgcgtgatgc tgcacgtggt gatgccagaa
     3241 tgtgcgcagt agttgaaatt ttattgcaaa caggtatgag aattagcgaa ttagcagcac
     3301 ttcagctgac tgatgtagat tttgagcgca atgtagtaac aattgttcct caaaattcac
     3361 gaggtggccg caaagttcca ctcaatactg cagctaaaaa agctttaatg gaatatcttg
     3421 aagtgcggcc acgtgccaga gaaaagacct tctttttgac caaaacctgc agaccatttt
     3481 tggtgagaaa cattagaact gcct
//
LOCUS       DAOQZP010000069         3430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_28798_length_3430_cov_4.604741, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000069 DAOQZP010000000
VERSION     DAOQZP010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28798_length_3430_cov_4.604741"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PKH60_04595"
     CDS             complement(<1..226)
                     /locus_tag="PKH60_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17351.1"
                     /translation="MEKQISVETKTTINFIQLFLTLCMLFAISSVLTSSNNYESERVS
                     NPSLYEIGHDVYSQVYAMMRSLNCPQPTFST"
     gene            complement(259..1050)
                     /locus_tag="PKH60_04600"
     CDS             complement(259..1050)
                     /locus_tag="PKH60_04600"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNQ17352.1"
                     /translation="MLEIEILFEDADLAVINKPSGITVNDASTNRGETIQQWWNSYSI
                     NQTIPQASSEWHSLIPPNFSLEYGTPEEIFSQRGGIVHRLDKDTSGALLLAKNPGSLV
                     DLLAQFKERLVGKTYQALVHGRLGVQRAELNYPTMRSSRERQKMFVHPDGRPSVTVYE
                     VVAEYQDPTQPTELLSKVECLPKTGRMHQIRVHFAHIRHPLVSDRLYAGRNRYKQDIT
                     WCPRHFLHASSIEFTHPRTKQVARVEAPLFDDLRKSLDLLQKYKS"
     gene            complement(1066..1470)
                     /locus_tag="PKH60_04605"
     CDS             complement(1066..1470)
                     /locus_tag="PKH60_04605"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNQ17353.1"
                     /translation="MRRRVTLFIFFCFCCLVADQLSKYMAFQQGYAVLNSGIAFGFLA
                     GAPWFFLITGIVALSILAFFFWHHQQGYPLLLLVFGASSNLIDRMQYGGVIDWLIVPG
                     LNIKNNLADWMVVIAVIWLLVVEFKNIRNESK"
     gene            complement(1467..2159)
                     /locus_tag="PKH60_04610"
     CDS             complement(1467..2159)
                     /locus_tag="PKH60_04610"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNQ17354.1"
                     /translation="MIYNIALVLALLCAGFVFWKRGKEEHFAEDQLFDGFLLSVLGGI
                     VVARFMYVLLHFDTFGWTLVSWLNIFSHGGLSLSFGFVSAIFLLIRFSKKQNWEFFGI
                     LDMWLPGVVLAAGVVQFGIFLQFQLQPAILVAGVLLLVMSRYLYWLEYRYRTFAWYKS
                     GQDVAKSGFITAVGLIFLGLIFLTVSFFPLGKSIALAEIVDRALCLFGVVFGFGLLLN
                     RSGVLAKNKKRQ"
     gene            complement(2185..2268)
                     /locus_tag="PKH60_04615"
     tRNA            complement(2185..2268)
                     /locus_tag="PKH60_04615"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2232..2234),aa:Leu,seq:caa)
     gene            2327..2581
                     /locus_tag="PKH60_04620"
     CDS             2327..2581
                     /locus_tag="PKH60_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17355.1"
                     /translation="MLRLTMQTIISDLVQTIFDPSSIWSVVARGAIWFVIAAVIIVSV
                     DSPNPNQSFRRMKSNLGFLVLFLVLSGTLMYMLFGYTKSA"
     gene            complement(2592..>3430)
                     /locus_tag="PKH60_04625"
     CDS             complement(2592..>3430)
                     /locus_tag="PKH60_04625"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNQ17356.1"
                     /translation="VESFERIDELQRSGEELRGVPTGFQDLDRILAGLQKSNLIVLAA
                     RPGMGKTALALNIAQHVSVHAKKKVGIFSLEMSKEELVDRLLVGQADIDAWRLKTGRL
                     DQQDFLKLSDAMGVLADAQLFIDDTPGLSIFDMRTKARRLMIEHSIDLLLVDYLQLAH
                     GRTMDNRVQEVSEISQGLKNIARELRIPILALSQLSRAIESRGEKTPQLSDLRESGAI
                     EQDADVVMFLYRKDEEKREAVSVKIAKHRNGPIGDIELYFRGERIKFYGMEKHREAMA
                     QH"
BASE COUNT         1041 a          803 c          604 g          982 t
ORIGIN      
        1 tagttgaaaa agttggttgg ggacaattaa gtgatcgcat cattgcatag acttgagagt
       61 acacatcatg accgatttcg tataatgatg gattgctaac tctttcgctt tcatagttat
      121 tagaagaggt taaaacgctc gaaatggcaa atagcatgca gagagttaaa aatagctgga
      181 taaaattaat cgttgttttt gtttcaactg aaatttgttt ttccatattt tttttgagga
      241 cggaaaacta tttgctggtc aactcttata cttctgaagt aaatcaagac ttttacgcaa
      301 atcatcaaac aaaggcgctt ctactctcgc aacctgcttg gttcttggat gagtaaattc
      361 gatgctactg gcgtgtaaaa agtgcctggg acaccaagta atatcttgtt tgtagcgatt
      421 gcgaccggcg tacagccggt cgctcaccag cggatgccga atatgtgcaa aatgcaccct
      481 aatctggtgc atccgtccag tctttggcaa acattcaacc tttgatagta attcagttgg
      541 ctgtgttggg tcctgatact cggctacaac ttcatatacc gtgacagatg gcctaccgtc
      601 tggatgcaca aacatttttt gtcgttcgcg tgaacttctc atggttggat aattcaattc
      661 agcccgctga acacccaatc taccatgtac taaagcttga tacgtttttc ccaccaacct
      721 ctctttaaat tgagcgagca aatccaccaa agaacctggg ttttttgcta aaagcaatgc
      781 tccactcgta tccttatcta gacgatgaac aatgccccct cgctgactaa atatttcctc
      841 tggggtacca tattccaaag aaaaattggg gggaattaat gaatgccact cactactagc
      901 ctggggaatt gtttgattga tcgaatacga attccaccat tgttggatcg tctcacctcg
      961 gttggtacta gcgtcgttta cagtaatccc agaaggttta ttgatcaccg ctagatcggc
     1021 atcttcaaat agtatctcta tctctaacat actctatagt atagtctact ttgactcgtt
     1081 ccttatattt ttgaattcaa ccactaacaa ccaaattact gcaatgacca ccatccaatc
     1141 agcgagatta ttttttatat tcaaaccagg aactatcagc caatcaatca ctccaccata
     1201 ttgcatccga tcaattaggt tgctacttgc tccaaaaact aagagtagca gagggtaacc
     1261 ttgctggtgg tgccaaaaaa agaaagccaa aatactcagc gctactatac cagtaattag
     1321 aaagaaccaa ggagctccag ctaaaaatcc aaaggcaatc cctgaattta gaacagcata
     1381 accttgttga aaagccatgt acttactcaa ctgatctgca accaaacaac aaaaacagaa
     1441 aaaaataaag agggttactc tccttctcat tgtcgctttt tattttttgc caaaacacct
     1501 gatcgattaa gtaacaaacc gaaaccaaac actacgccaa aaagacaaag tgctcggtcc
     1561 acaatctcgg ctaaagctat cgatttacct aatgggaaaa acgaaactgt caaaaatatc
     1621 agcccaagga aaatcaggcc aacagcggtg ataaatcccg atttagctac atcttggcca
     1681 gatttatacc aagcaaaagt tcgatatcta tactccaacc aatacaaata gcggctcatg
     1741 actaacagta acacgcccgc tactaagata gctggttgca actgaaattg taagaagata
     1801 ccaaattgca ccacaccagc tgccaatacg actccgggta accacatatc aagaattcca
     1861 aaaaattccc aattttgttt tttgctaaac cgaatgagta aaaagattgc agagacgaat
     1921 ccaaaggata aagaaagacc accgtggctg aaaatgttta accaagaaac caaggtccaa
     1981 ccaaatgtat caaaatgcaa taagacatac ataaatcgtg ctactacaat ccctcccaaa
     2041 accgaaagta aaaatccatc aaataactga tcttccgcaa agtgctcttc tttaccacgc
     2101 ttccagaaaa caaatccggc gcaaagtaag gctaaaacca aagcaatgtt gtagatcata
     2161 gttttttgaa ttttattatt cttgtgccga gagagggaat tgaaccctca ctcccttgcg
     2221 ggaatcggtt tttgagaccg acctgtctac ctattccagc acctcggctt tgttcatgct
     2281 acacttatag tatagagcga aacaattgta tcatcgcctt gcttttttgt tgcgactaac
     2341 catgcaaaca attattagcg atctagttca aaccatattt gatccaagta gcatttggtc
     2401 cgtagttgcc agaggagcaa tctggtttgt aatcgctgct gttattatcg tcagcgtgga
     2461 ctcaccaaat cccaaccaat ccttccgtcg tatgaaaagc aatcttggtt ttctagtact
     2521 ctttctggta ctctcaggca cactgatgta tatgttgttt gggtatacaa aatcggcgta
     2581 attttttgtt cctaatgctg tgccatcgcc tcacggtgtt tttccattcc gtaaaatttt
     2641 attcgttcgc cacggaagta taattcaata tctccgatag gcccatttcg gtgtttcgca
     2701 attttgacgc tcactgcctc gcgtttttct tcatcttttc ggtacaaaaa catcactacg
     2761 tccgcatcct gctcaatagc acccgattct cgcaagtctg agagttgagg tgttttttct
     2821 ccccgacttt caattgcacg agaaagctgg gaaagtgcca aaatggggat acggagctct
     2881 cgtgcaatat tttttaaacc ttgcgagatt tcactcacct cttgcacccg attgtccatt
     2941 gttctaccgt gagccagttg caaatagtca actaacagta agtcaatcga gtgctcaatc
     3001 atcaaacgac gggcttttgt gcgcatatca aatattgaaa gtcctggggt atcatcaata
     3061 aataattgcg catctgccag gactcccata gcatcagata atttcaaaaa gtcttgttga
     3121 tctaatctac ctgtttttaa tctccaagca tcaatatctg cctggccaac caataaacga
     3181 tcaactagtt cttctttgga catttcgagt gaaaaaattc caaccttctt ctttgcgtgc
     3241 acgctcacgt gttgcgcaat gtttaaagcc aaagcagtct tccccatacc tggcctggct
     3301 gccaacacaa ttaaatttga cttctgtaac ccagctaaaa ttcgatctag atcttgaaat
     3361 ccggttggta cccccctcaa ctcctctcca gatctttgca actcatcaat tctttcaaaa
     3421 ctttccacca
//
LOCUS       DAOQZP010000070         3355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_29772_length_3355_cov_4.835758, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000070 DAOQZP010000000
VERSION     DAOQZP010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29772_length_3355_cov_4.835758"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            735..3326
                     /locus_tag="PKH60_04630"
     CDS             735..3326
                     /locus_tag="PKH60_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17357.1"
                     /translation="MDDGIFHDISMYDEVELKLCVLGIDSLGTEQELNQATEFVWTRV
                     VPTYVYFASSGETFTEGVASLTFKFFSTKPAGIPIIIDYDIYGDLVGIMNYQRGSVTI
                     LSGQNEATLVLPVNSNPSPNTDKRVTVGITNLNGAYRIDGTLSLSSQLILDAQRPVVG
                     VLDIKSNCAILTDGKLVCTGDIGGPTSSTRNWAYGYRNIIPGVSFKKIISAGYVYIAQ
                     SVDDELYSWGWGFNGDLGLGSGADKKVPEKINGFVWNKVEKSGVQCGITVSEDLYCWG
                     DHTYTSGALGIASSADARSPAQVEPGNKFIDIFMYGNSNCAIEKTTHALKCWGSNYGN
                     ILGTGNSTNPNVPVIIDSGVQYKQVKFGYNYTVGLTTAGAIKIWGDNPPISQYASPTP
                     VLVDESRNYSRIVIAGPKLICFLTDQDDLYCLGNYGGNNLGFDEEFVLPTLIDEGDKY
                     SKFVSTGDIGCGLTYTNKVKCFWHGWSFYSSLLPKGPEEWASGTYSDIIADETRGRIC
                     VLDATSFPKCIFSNTHDPAPSYASVLSDSTFNIRQAWYDYILDENFKVYTFTHSGANT
                     TGAMVRAVDFPTDQNIKFLSNRGNCFINTSDKLFCRGFYRGDGGSSTGYESQFVSVLP
                     DKNIVAVQQLTFSYQNTCALTDQKKLYCWGLNSYGEMGKGDIVESNTPTLVMSQVIDF
                     DTGVALVCAINESFELYCWGTSSSGIGNGSTSSFSPTRIGGTDLFTKVKSGRVNCAIR
                     QDNGRVMCWGATYGIGNGSSASRATPTLTSDTDSYQKIFAYGSGTCGVTSGIIRCWGE
                     FAGLGTLVPVLVHNGDIKFKSEYIVSPEGKLFGLGWSTRFFGQQGFPFFEPFLGLVDR
                     SIPDATF"
BASE COUNT         1014 a          578 c          756 g         1007 t
ORIGIN      
        1 ggagcattcc agacgccact ttctaataaa tgtactttcg catttttaca atgagtctac
       61 ctttacaaaa cgagttcacg aattcctaag aagttaccac aacttcttag gaattcgtga
      121 tgtttgaggc tttgtgaagg tagactcttt gtaaaaacaa cgaactcgtt ttttaaagat
      181 atctagaaaa tcttaaatca tagtgccttc tcaaaaactt ctctttcaga atgagacatc
      241 aatctgtgag aggaaaaaag ataatggaat aaaatagttt taagttcata aagaatagtc
      301 agaattctca ttttgagaaa tttaagagag gactttggtc cggcttaaaa taatccgata
      361 ttatatttat gctaatgttc agaaagagag tccagttagt tttggcttca atttgtattt
      421 ctttaatggt ttcagcttgc gatttgaaca gcctgattga aaagaacatt cgaaatcctt
      481 ctgaaaatcc cactttatac ccaccaccga tctcagaacc tgaggagcag aatccgggtg
      541 gtggcgatcc agacgatagc aatacgagtg gggttcccgt agccatcttg ctttacacgc
      601 ccacagacgg aggttccgaa aactatttag atacttacgt agaaggtgat ggaatcgtaa
      661 actatcgtta caagtatggt ccaagcgtat cgacgaattg caatcttgat attggatatt
      721 cggctgtcca atctgtggat gatggtattt ttcatgacat cagtatgtat gacgaagtgg
      781 agctcaaact atgtgtattg ggtattgatt ctttgggaac agaacaagaa ttaaatcaag
      841 ccacggagtt tgtgtggacg agagtcgtac cgacctatgt ttattttgca agttctggag
      901 agacttttac agaaggtgta gccagtttaa ctttcaaatt cttctctacg aaaccagcag
      961 gaattcctat cataattgat tacgatatct acggagattt ggtcgggatt atgaattatc
     1021 aaagaggatc cgttacgatc ctctcaggtc aaaatgaggc aactttagtt cttcctgtta
     1081 actccaatcc aagtccaaac actgataaaa gagtgactgt tggaataaca aatttaaacg
     1141 gagcttatag gatagatggg actttatcac tttcctccca acttatactg gatgctcaaa
     1201 ggccggtcgt aggtgtctta gatataaaat caaactgtgc cattttaacc gacggtaaat
     1261 tggtttgtac tggtgatatt ggcggcccca catcatcaac aagaaattgg gcctatggat
     1321 atagaaatat tattccagga gtctcattta agaaaattat ctcagcggga tatgtttata
     1381 ttgcacaatc tgtagacgac gaactatatt cttggggttg gggatttaat ggcgatttgg
     1441 gattaggttc gggtgcagat aaaaaagtac cagaaaaaat aaatggattt gtatggaata
     1501 aggttgaaaa atcaggagtg caatgcggta tcactgtttc ggaagaccta tattgttggg
     1561 gagaccacac atatacgagt ggagcattag gaattgcatc gtcggcagat gcaagaagcc
     1621 cggcacaggt tgagccaggt aataaattta tagatatttt catgtatgga aatagcaact
     1681 gcgctataga aaaaacaact catgcactta aatgctgggg gtccaactac ggtaatatac
     1741 ttggtactgg aaactcaaca aatcctaatg tccctgtaat tatcgattcc ggtgttcaat
     1801 ataaacaagt aaaatttggt tataattata ctgtaggatt gacaacggcg ggtgctatca
     1861 aaatttgggg agataatcct ccaataagtc agtatgcaag ccccacacct gttcttgttg
     1921 atgaaagtcg aaactattca agaattgtca tagctggtcc caagttgatt tgttttttaa
     1981 cagatcagga tgatctgtat tgtcttggaa attatggagg taataatctt ggtttcgacg
     2041 aggagttcgt gttgcctaca ttaatagacg aaggggataa gtattcaaag tttgttagta
     2101 caggtgatat aggatgtgga cttacttaca ccaataaggt gaagtgtttt tggcatggct
     2161 ggtcttttta ttcaagtctt ttgcccaagg ggcctgaaga gtgggctagc ggtacttatt
     2221 cagatattat tgctgatgag acaagaggtc gcatttgtgt tttagatgca acaagttttc
     2281 ctaaatgtat attctcaaac acgcatgatc ccgcaccaag ttatgcaagc gtgttgagcg
     2341 actcgacgtt taatattagg caggcttggt atgattatat tctagatgaa aatttcaaag
     2401 tttacacctt tacccactct ggagcgaaca ctacaggagc catggtcaga gcggtagatt
     2461 ttcctactga tcaaaacatt aaatttttaa gcaatcgagg caattgcttt attaatacga
     2521 gtgataagct tttttgccgt ggattttata gaggagacgg tggttctagt acggggtatg
     2581 aaagtcaatt tgtgagcgtc ttaccagata aaaatattgt ggcggttcaa cagctcacgt
     2641 tcagctatca gaatacctgc gcgctcacag atcaaaaaaa gctgtattgc tggggattaa
     2701 attcatatgg agaaatggga aaaggagata ttgttgaaag caatacaccc actcttgtta
     2761 tgagccaagt tatagacttt gatacgggag tggcattagt ctgtgccata aatgaaagct
     2821 ttgagctcta ctgctgggga acctcttcga gtggtattgg gaatggatca acaagctcat
     2881 tctctccaac gcgaattggc ggaacagatt tgttcactaa agtaaaatct ggaagagtca
     2941 attgcgctat tcggcaggat aatggccgag tgatgtgttg gggagccact tatggaattg
     3001 gaaatggatc ttctgcaagt cgtgccaccc ccactctgac aagtgataca gacagttatc
     3061 agaagatttt tgcatatggt tcgggaacct gtggagtgac ctctgggata attagatgtt
     3121 ggggagagtt tgcggggttg ggaacactgg ttcctgttct cgtacacaat ggagatatta
     3181 aattcaaatc tgaatacata gtttcacctg agggaaaatt attcggtctt ggatggtcca
     3241 cgcgattttt tggacaacaa ggattcccgt tttttgaacc tttcttaggc ttagtcgaca
     3301 ggagcattcc agacgccact ttctaataaa tgtactttcg catttttaca atgag
//
LOCUS       DAOQZP010000071         3241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_31322_length_3241_cov_5.245135, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000071 DAOQZP010000000
VERSION     DAOQZP010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31322_length_3241_cov_5.245135"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PKH60_04635"
     CDS             <1..522
                     /locus_tag="PKH60_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNQ17358.1"
                     /translation="ELNISCPNTFGGEPYTTCPRLESLMDAIDKIKLSKPLYVKMPID
                     QSEAETKELLSVLNKHNVQGVIFGNLTKDRSNPLVTEADRLEWKKLKGNLSGKPTWER
                     SNSLISLTKKHYKNRFTIVGTGGIFTAEDAQHKLDLGADLLQLITGMIFGGPQTVGTI
                     NLGLALKISSPSD"
     gene            complement(497..1033)
                     /locus_tag="PKH60_04640"
     CDS             complement(497..1033)
                     /locus_tag="PKH60_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNQ17359.1"
                     /translation="MLDLISFNAKICYTAAGQVYHDNKLLLVRHRKLNLWLAPGGHLD
                     PEELPHIGAEREVFEETGVKAIAFDPNPLLASTTSTYIPSPIISNVHWVCEENYQRRL
                     RDGDAYKRDPQWQKGCEQHLGFVYLMRVIGSTSVTFNERESTDIGWFTQEEISALHTT
                     EDLKREFNYGFSLMAKIS"
     gene            complement(1035..1568)
                     /locus_tag="PKH60_04645"
     CDS             complement(1035..1568)
                     /locus_tag="PKH60_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNQ17360.1"
                     /translation="MQYKFDPRLLKSAVFGANDGIVTTFAVVAGVAGAHLSAQVVLIL
                     GVANMIADGLAMGLGDYLGERSERQMRNHGSKVVGDGIWHTGLITFVAFVIAGSLPLL
                     PYIANSIGLPVAASMQFPLSIFTTASALFCVGALRTVFTKCSVWRGGLEMLGIGAIAA
                     VAAYVFGALVEKALVGM"
     gene            complement(1558..1947)
                     /locus_tag="PKH60_04650"
     CDS             complement(1558..1947)
                     /locus_tag="PKH60_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNQ17361.1"
                     /translation="MTGYIGKIEQLTLNNTAFRQVVFTGKHAQLVVMSLLPREEIGEE
                     VHNAVDQFFRVESGTAKVVMNGEESVVSNGDAFVVPAGTVHNVINVSTDQPLKLYTIY
                     SPPNHPEGTVHQTKAEAEAAHESEHAV"
     gene            2001..>3241
                     /locus_tag="PKH60_04655"
     CDS             2001..>3241
                     /locus_tag="PKH60_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HNQ17362.1"
                     /translation="MSDTILLELRTSRDSLDAPSVAASIFHALPQRGTFSSGLLFNRA
                     SISFEISSLLQSTRFSIAVPAKHESYIQALATSAYQSALINIPEYDPLQTIQAWQLHA
                     NVAPLVVEIKPSIFSSSSEIKSLEAFTEGDSLAPLLSFFSKTDQETIVVFQMIVKTKK
                     QSKLLRQISRQVNSQTSNQTAGESEKPDPINQALQATLRFGIWTPSVTRTQSVFEALS
                     AIVMSWSADLSHPLTLSKPIGQKRAFKAMLQRKAGKHWQITPSQAATLYHLPHKGLST
                     IHNIHWGKLLPGEPPENLPIVTKDLVPEARRELNVVGKTTFKNQERVFAIKRADRRRH
                     IYVIGKTGTGKSTLLANMAINDLKNDEGICVIDPHGDLVETLLDYIPSRRVNDVIYFD
                     PADTGKTVKINLFEGENLVHR"
BASE COUNT          973 a          791 c          660 g          817 t
ORIGIN      
        1 gaactcaata ttagttgccc aaataccttt ggtggcgaac cctacaccac ctgtcccagg
       61 ctagaatcac tcatggacgc gatagacaag attaaactga gtaagccact ctatgtcaaa
      121 atgcccattg atcaatctga ggcagaaaca aaggagctgc tttctgtgtt gaacaaacac
      181 aatgtgcagg gggtaatctt tggcaacctc acaaaagata gaagtaatcc attggtaact
      241 gaagctgacc gccttgaatg gaaaaaactc aagggcaact tgagcggcaa accaacgtgg
      301 gaacgatcga acagcttaat ttccctgaca aaaaagcact ataaaaatcg cttcaccatc
      361 gttggcactg gaggaatttt taccgccgag gatgcacaac acaaactaga tcttggcgca
      421 gacttactcc aattgattac aggaatgatt tttggagggc ctcaaacagt gggaacgatc
      481 aacctcggtt tagctcttaa gatatcttcg ccatcagact aaaaccataa ttgaattctc
      541 gcttaagatc ttcagtagtg tgaagagctg atatttcttc ttgggtaaac cagccgatat
      601 ctgtactctc tcgctcattg aaggtaacag aggttgatcc aattacccgc attaaataca
      661 cgaatcctag atgctgctca caaccttttt gccattgtgg atcacgctta taggcatctc
      721 catctctgag ccttctttga tagttttctt cacagaccca gtgaacattg ctgatgatgg
      781 gggaagggat atacgttgag gtagtagatg caagcaacgg atttggatca aaggcaatcg
      841 ccttcactcc ggtttcttca aaaacttcgc gttctgcgcc tatatgaggc aattcttcag
      901 gatctagatg accgccgggg gctagccaga gatttagctt acgatgccgt accaataaca
      961 acttattatc gtgataaacc tggccggcgg cggtatagca aattttggcg ttgaaagaaa
     1021 ttagatcgag catgttacat tcctaccagg gctttttcaa ctagtgcacc aaacacatac
     1081 gcagcaacgg ctgcaatcgc cccaattccc aacatttcga gtccaccacg ccaaacgcta
     1141 catttagtaa aaacagtgcg tagggcaccc acacaaaaaa gtgcactcgc agtcgtaaag
     1201 attgaaagtg ggaattgcat cgatgctgct acaggtaaac caatactatt agcaatatag
     1261 ggtagtagtg gcaatgatcc cgcaatgaca aatgcaacaa aggtaattaa tccagtgtgc
     1321 cagataccgt ctccaacaac tttggagcca tgattacgca tctgacgttc agagcgctca
     1381 cccaaataat cgcccaaacc catcgccaaa ccatcggcga tcatattagc tacaccaaga
     1441 atcagcacta cttgagcaga aaggtgggcg ccggcaaccc cagcaaccac agcaaaagtc
     1501 gtcacaatgc catcattggc cccaaacacc gctgatttta atagtcgagg atcaaactta
     1561 tactgcatgc tcagattcgt gtgccgcttc ggcctcagct tttgtttgat gaaccgttcc
     1621 ttctggatga ttgggtggac tgtagatggt gtacaacttc aacggttgat ctgtggaaac
     1681 attaatcaca ttatgtaccg tgccagcagg caccacaaaa gcatcgccat tggatacgac
     1741 tgattcctca ccattcatca cgactttggc agttccagat tcaactctaa aaaattggtc
     1801 aactgcattg tgaacttcct caccaatttc ttctctgggc aataatgaca tcaccaccaa
     1861 ctgagcgtgt ttgccagtaa acactacttg ccgaaaagca gtattgttca aagtaagttg
     1921 ctcaattttt ccaatataac cagtcataat agactccttt ctttaccctc aactatacca
     1981 cgcgatgcta tactggatga gtgtcagata ctatcctact agaattacgt accagccgag
     2041 attccctcga tgccccaagt gtggctgcta gcatttttca tgctttaccc caacgaggca
     2101 cattttcttc tggtctatta tttaatcgag cctccatcag ctttgaaatc agctcactcc
     2161 ttcaaagtac tcgcttttca atagcagtgc ccgccaaaca cgaatcatat atccaagccc
     2221 tggctaccag cgcctaccaa agtgccttaa ttaacatccc agaatatgat ccattacaaa
     2281 ccatccaagc ctggcagctg cacgctaacg tggcaccact ggttgtagag ataaaaccaa
     2341 gtattttttc tagctcaagt gagatcaaat ctctagaggc atttacagag ggtgattcac
     2401 ttgcaccgtt attgtcattt ttttctaaaa ctgatcaaga aactattgtg gtgtttcaga
     2461 tgattgtgaa aaccaagaag cagtccaagc tacttcgaca aattagcaga caagttaatt
     2521 cccaaactag taatcagaca gcaggcgaaa gcgaaaaacc agatccaatt aatcaggcgc
     2581 ttcaagcaac gctcagattt ggcatctgga ctccctcagt tactcggacg cagtctgtgt
     2641 ttgaagctct gagcgcaatc gtgatgagtt ggagcgccga tctttcacat ccacttacgc
     2701 tatccaaacc aattggccaa aagcgtgctt tcaaagccat gcttcagaga aaagcaggca
     2761 agcactggca aattacacca agccaggctg ccacactgta tcatttacct cacaagggtc
     2821 tttctaccat tcataatatt cattggggta aactgctccc cggagagcca ccagaaaatt
     2881 tgcctatcgt gacaaaggat ctggtgccag aagcgcgtcg agaattgaat gtagtgggga
     2941 agacaacatt taaaaatcaa gaacgcgttt ttgccatcaa gcgagccgat cgcagacgtc
     3001 atatatacgt gattgggaaa acaggtactg gtaaatcaac tttgcttgcc aatatggcca
     3061 ttaatgacct caagaatgac gagggtattt gtgtgatcga tccacacggt gatctggtgg
     3121 aaacactgct ggactacatt ccttcccggc gagttaacga cgtgatttac ttcgacccag
     3181 ccgatactgg caaaacggta aaaatcaatt tgtttgaagg agaaaatctc gtccatcgag
     3241 a
//
LOCUS       DAOQZP010000072         3234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_31413_length_3234_cov_7.035231, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000072 DAOQZP010000000
VERSION     DAOQZP010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31413_length_3234_cov_7.035231"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3234)
                     /locus_tag="PKH60_04660"
     CDS             complement(<1..>3234)
                     /locus_tag="PKH60_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17363.1"
                     /translation="TRTTSYLLLQPTAGNVGIGSASPAQKLDVVGNVQFSGALMPNGQ
                     AGTSNYLLTSAGAGSPPTWQNPATLIPAYTLWSQNLGALYPKNSTVDFLLGATSTASA
                     KMSVINLNSGTPTMEIKGDFKWGGLGSNKIGTLTSNTTQTTMRAPAGVAMGIGANGGN
                     SDIYISALNNVGIGTTTPTALLDIAGAATSSATLSFRGTTDPKINVLNGENFGIQTSV
                     GGDAGLTERLTILNGGNVGIGSTTPSQKLDVAGSLTLGGGESILFTGTSNKIVNASGT
                     DLGFQTSNQWNVFLTAGGNVGIGGTAANSTPTLLATSAGNVGIGTTNPNNFKLQVNGN
                     VGAQTNNTSTLGSSSVRWANIYSVLGNYSGQITSSLATGTSPFAVSSTTVNTNLNADL
                     LDGQHGSYYLPAGNVSGTTNYVAKFTGTNSVGNSNIFDNGTNVGIGTTSPNTKLHLLG
                     DLTITGGIRPATDGTTALQLQKANGTSVLDVDTTNSNVLVSGKLIANGFVIDKTNQDM
                     LISRHTGGEVAFRSQTTNGRSQIILSPNGAPTYSRARMMFGLTDIIADPTNVENMTFA
                     ARGDTNEYWISTEAIGTGTARPIVFKRYQQGASPSTIDIMRLMTDGNVGIGTTTVPDL
                     LTVRTTTNDVGITLAGASSGVSPTYKLADSGGTVHARIGIAGAAGNLVTNASINDLVF
                     RNNAKNIHFTTDNGTSTSLFVGSNGRVGIGTTSPSYAFQANGPVDTNIISAQVTSSNR
                     KIGLAVGDSRVGLHLRNDGTSHMGISSDGSDIFFGNASSSESPDNWGADLMTIKNGGN
                     VGIGTTGPTQKLDVNGNVILSATSPYLYFENTNNYLLSSSDNLISKIAGSYYFRDTNS
                     YDRLSILSTGDTTINNASNGNIATFKNSGNVGIGTTSPTAKLQVAGTLLVQPTGNSTS
                     SLTLAPGGTSNRSFLSIFATSDTTTNYTKLYTDQALGAHAFITTAGGTGTQYPISFNS
                     TLTNPLLYINPNGNIGIGGTDAGATPKLFVDGSSGNVGIGTTNPTSLLHINGSGAMMK
                     IVATANDPVLFFNNLSQNWYSGIDTSDSNKFKIATSTLGSGYDMFVI"
BASE COUNT          868 a          678 c          724 g          964 t
ORIGIN      
        1 taatgacaaa catatcataa ccagacccta gagtgctggt agcaatttta aacttattac
       61 tatcactagt atctatgcct gagtaccagt tttgggataa attattaaaa aatagaacag
      121 gatcattggc agtggcaact attttcatca tagccccact gccattaata tggagtaagg
      181 aggtgggatt agtagtccca atcccaacat tgccagagct accatctaca aaaagttttg
      241 gagttgcgcc agcatcagtt cctccaattc caatatttcc atttgggtta atatataaaa
      301 gtgggttagt gagagtagag ttaaatgata tgggatattg tgtgcctgtg ccgccagcag
      361 tagtaataaa tgcatgagcg ccaagcgctt gatcagtgta gagtttagtg taattggttg
      421 ttgtgtcaga tgtggcaaaa attgaaagaa atgagcgatt tgaggttcca cctggagcaa
      481 gagtaagact actagtacta tttcctgtag gttggactaa caatgtacct gcgacttgta
      541 gttttgcagt tgggctcgtc gttccgatac cgacattgcc ggaattcttg aaagtggcga
      601 tattaccatt tgaagcatta tttattgttg tgtcacctgt tgacagaatc gataacctgt
      661 cataggaatt tgtatctctg aaataatatg agccggcgat ttttgagatc agattatctg
      721 agctggacag tagataattg tttgtgtttt caaaatataa ataaggtgaa gttgcggata
      781 gaattacatt gccatttaca tctaatttct gagtaggccc tgtcgtcccg ataccaacat
      841 tgccaccgtt tttaattgtc attaaatcag caccccaatt atctggactt tcactactgc
      901 ttgcatttcc aaaaaagata tcgcttccat ctgaggaaat tcccatatgt gatgtaccat
      961 cgtttctcaa gtgcaagcca actctgctat ctccaactgc taagccaatt tttctattac
     1021 tacttgtgac ttgcgcagaa ataatattcg tatcaactgg tccatttgct tgaaaagcgt
     1081 atgatggact tgttgtcccg attccaactc tgccgtttga gcctacaaac aaacttgtgg
     1141 atgtgccgtt atcagtggtg aagtgaatat tcttagcatt atttctgaag actaagtcat
     1201 ttatcgaggc atttgtaact aagttaccag ctgcacctgc aattccaata cgtgcatgaa
     1261 cagttccacc gctatctgcc aatttataag tgggtgaaac cccagaagat gctccggcca
     1321 aagtgattcc tacgtcattt gtagttgtgc gcacagtcaa aaggtctgga acggtggttg
     1381 taccgatacc tacattgcca tctgtcatta gcctcataat atcaattgtt gatggacttg
     1441 ctccttgttg atagcgttta aacacaatag gtctggcggt accagtccca attgcttcag
     1501 tggaaatcca gtactcattt gtatcccctc ttgcagcaaa cgtcatgttc tctacattgg
     1561 ttggatcggc aattatatct gttaatccaa acatcatgcg tgctctagaa tacgttggag
     1621 ctccatttgg tgagagaata atttggcttc ttccattcgt ggtttgggag cgaaaagcaa
     1681 cttcaccacc agtatgtcta gaaattagca tgtcctgatt tgttttatct ataacaaatc
     1741 catttgctat taattttcca gatacaagca cattactatt cgtagtatcg acatctagaa
     1801 cagaagttcc atttgccttt tgtagttgta aagctgtggt gccatctgtt gcaggacgaa
     1861 tacctccagt tatggttaga tctccgagta agtgcaactt tgtgttagga ctcgtcgtcc
     1921 caatgccgac gttagtgcca ttgtcaaaaa tattcgaatt acctacagaa ttagtgccag
     1981 tgaatttggc tacgtagttg gtggttcccg aaacattacc cgctggtaag tagtagctcc
     2041 catgttgacc atccagcaaa tcagcattta gattggtgtt gacagtggta gatgagacag
     2101 caaatggaga agtgccagtg gcaagagaag aagtaatctg acctgagtag ttaccgagaa
     2161 cactatatat attagcccag cgcacactgc tactacctag ggtagaggtg ttattagttt
     2221 gtgcaccaac attaccattc acttggagct taaagttgtt aggattagtc gtcccaatgc
     2281 cgacgttgcc agcgctagtc gctagtagag ttggagtgct atttgccgca gtaccaccga
     2341 tccctacatt tccgcctgct gtaaggaata cattccactg gttggatgtt tggaacccaa
     2401 gatctgtccc actagcattg acaatcttgt tggatgttcc agtaaacaaa attgattcgc
     2461 ctccacccaa tgtcaaactt ccagcaacat caagtttttg acttggtgtt gttgagccaa
     2521 tacctacgtt gccaccattc aaaattgtga gtctctcagt cagtccagcg tctccgccaa
     2581 cgctggtttg aatgccgaaa ttttctccgt taagtacatt gatcttgggg tcagtagtgc
     2641 cacggaacga gagggtggct gaggaagtgg cagctcccgc aatatcgagt aacgctgtgg
     2701 gggtggtggt gccaatacca acattattaa gcgcgcttat atatatgtcg ctattaccac
     2761 cgtttgcacc aatgcccata gctacaccag cgggtgctct catggtagtt tgagtagtat
     2821 tagatgtaag tgtgccaatt ttgttggaac caagtccacc ccacttgaaa tcacccttga
     2881 tttccatagt tggtgtgcca ctgtttaggt tgatcactga catttttgca gaagcagtgg
     2941 aagtggctcc aagcaagaaa tcaacagtac tattcttcgg gtagagagca cctaagttct
     3001 gactccagag cgtgtatgca ggaatgagtg tggcgggatt ctgccaggtt gggggagagc
     3061 cagctccagc tgaggtgagc aagtaattac tggtcccagc ctgcccgttg ggcattaggg
     3121 cgccactgaa ttggacatta ccaaccacgt ctagtttttg agcgggagat gctgagccaa
     3181 tgcctacatt gcctgctgtg ggttggagga gtagataact agtcgttctc gtag
//
LOCUS       DAOQZP010000073         3213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_31710_length_3213_cov_4.432236, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000073 DAOQZP010000000
VERSION     DAOQZP010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31710_length_3213_cov_4.432236"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            251..1255
                     /locus_tag="PKH60_04665"
     CDS             251..1255
                     /locus_tag="PKH60_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HNQ17364.1"
                     /translation="MKFNFHIFIQSKKLLLTAFFLVFAVGVGFGQLMSLGLQKPRSTN
                     SDRIHLNPSYSYINPLLECDSAVENFGSFQRVEGKLEEYIDSEVRKGHISATSVYLRD
                     LNNGPWVGINEKDEFSPASLIKVPLLIAYLKQAEENPSLLEIPLTINNIDAYKDQNLI
                     PSTLLGLGQQYKTIDLLRQMIVYSDNVAYEALNANFSGPDLIATYQDFGIDISKGLSD
                     PSGNILSVKDYASFFRILFNASYLSEKNSELALEILTQSKYEDGLRAGVPSNIAIAHK
                     FGERRYLLTSETQLHDCGIVYLPENPYLLCVMTKGTDFNLLTSVIAEISRIVYLNVSQ
                     "
     gene            complement(1245..>1732)
                     /locus_tag="PKH60_04670"
     CDS             complement(1245..>1732)
                     /locus_tag="PKH60_04670"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17365.1"
                     /translation="DIVDSAGNVVGYHKGLWFYTIGQRHGFTINQTSLVKNQLGEMVT
                     KHNIPPFYVIAKNSEKNQLVVGFGEEAKSNQFFLAEMRSIDPNLDLLTIENPLIRIRH
                     GGKLLSCKLEKVGEKWQVTLESAIQGISEGQFGVLYQELQDNVFCLGGGIIGLSTNTT
                     D"
     assembly_gap    1733..1742
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1743..2358)
                     /gene="mnmA"
                     /locus_tag="PKH60_04675"
     CDS             complement(<1743..2358)
                     /gene="mnmA"
                     /locus_tag="PKH60_04675"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNQ17366.1"
                     /translation="MKIALGLSGGVDSAVCAHLLVEQGHAVSTVFLECWNEPGCRTDQ
                     DRKDALSIALSLKLPFQVLDFKQAYRDKVLQYFLDEYQVGRTPNPDVVCNREIKFGLF
                     YEWAMAQGFDAISTGHYAKIAAINDQKQLVIPKDTHKDQTYFLSQINQYQLNHVIFPL
                     AELEKNEVRTLAIQKNISVANKKDSVGICFIGDINVHTFLKERLG"
     gene            complement(2355..3140)
                     /locus_tag="PKH60_04680"
     CDS             complement(2355..3140)
                     /locus_tag="PKH60_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17367.1"
                     /translation="MPTMHFTPLWRPQKVSWVYPTTSDKSVLENIRSKNARKFVSTIP
                     EFLQRNKFELSSGACTFKEYREFLELYNERTAARGFDPIAKVEWFSAKKREGKKVEKI
                     FIRHNDQLIGGKIITVIGDEVRSSYKASQQLPIFQKLHNASLGLILDYSMLDHYTKKK
                     PSLLTAGTSRNLFGVFNTIGYLTFKLRMGYQPQVINPKTIFEQTTKDPHKDIWLAFLC
                     DSATIKQPLTLYYSGELDQIPQLAELQKLISLEKLTDAVQYKS"
BASE COUNT          950 a          595 c          631 g         1027 t
ORIGIN      
        1 ctagtatcct aaaagttcag gacttgatct ctagagcgga ggggattata acagcgtata
       61 atccaattag tcaacagcaa ggttgatgta ccccaaacgc acacctattg agtacagacc
      121 atcagcatag ataacttaag aaaggtcagc tatggagaaa aaattactcc aatggatttt
      181 actatactag cgaaaaagtc gctagtaagt agcagattgt aaaatttatt agatccgtta
      241 atataagaaa gtgaaattta attttcatat ttttattcaa agcaaaaaat tgttgctaac
      301 tgcatttttt ttagtatttg cagttggtgt gggttttggc caactgatga gtcttggttt
      361 gcaaaaacca cgctctacaa attcggatcg tattcattta aacccttctt attcatacat
      421 taatccttta ttagaatgtg attctgcggt tgaaaacttt ggctcatttc aacgagtaga
      481 gggaaagcta gaagagtata tcgattctga agttcgtaaa gggcatatta gtgcaacctc
      541 agtttatcta cgagacttaa ataatgggcc ttgggttggg attaatgaaa aagatgaatt
      601 ttcgccggcg agtttaatta aagtacctct cttgattgct tacttaaaac aagccgaaga
      661 aaatccttcg ttattagaaa tacccctgac cataaataac atagatgcct ataaggatca
      721 aaacctgatt ccaagtacgc tacttggatt gggtcagcaa tataaaacca tcgatctatt
      781 gaggcaaatg atcgtctatt ccgataatgt agcctacgag gctctaaatg caaactttag
      841 cgggccggat ttgattgcaa catatcagga ctttggtata gatatatcta agggattatc
      901 agatccttcc ggaaatattt taagtgtcaa agactacgca tctttttttc gaatattgtt
      961 taatgcatcg tacttgagtg agaaaaattc cgaattagcg cttgagatat tgacgcaatc
     1021 taagtatgaa gatgggttgc gagctggagt tccgtctaat attgctattg ctcacaaatt
     1081 tggtgagcgt agatacctat taacctcaga aacacagttg catgattgtg gcatagtgta
     1141 tctgccagaa aatccgtatt tgttgtgtgt aatgactaaa gggacagact tcaatctctt
     1201 aacttcggtg atagctgaga tttctcggat agtttattta aacgtcagtc agtagtgttt
     1261 gttgaaagac caattattcc tcctccaagg caaaaaacat tgtcttgtag ctcttgatag
     1321 agaaccccaa actggccttc ggatattcct tgaattgccg actcgagtgt tacctgccat
     1381 ttttcaccga ctttttctag cttacaacta agcaactttc ccccatgacg gattcttata
     1441 agtggatttt ctatggttaa aagatcaaga tttggatcaa ttgagcgcat ttctgctaag
     1501 aaaaattgat ttgatttggc ttcttcacca aaaccaacta ctaattgatt cttttcagaa
     1561 tttttagcga tgacatagaa tggtggaata ttatgttttg ttaccatttc cccgagttgg
     1621 ttcttcacca aactggtttg attgatggta aatccatgac gttgaccaat ggtataaaac
     1681 cataaaccct tgtggtaacc cactacgttt ccagccgaat caacaatatc tcnnnnnnnn
     1741 nncacccaat cgctctttga ggaaagtgtg aacattaata tcaccaataa aacaaatgcc
     1801 aactgaatct tttttgttgg cgacagaaat atttttttga attgctagtg ttctaacttc
     1861 gtttttttct agctcagcta gcggaaaaat cacgtgatta agctgatatt gattaatctg
     1921 agataaaaaa taggtttgat ctttgtgggt gtccttgggg atgacgagct gtttctgatc
     1981 gttaattgca gcaattttag cgtaatgtcc ggtggagatg gcgtcaaagc cttgcgccat
     2041 cgcccactcg taaaataaac caaacttaat ttctctattg cagactacat cggggttggg
     2101 tgttctcccc acttgatatt catctaaaaa atactgtaac actttgtcac gatatgcctg
     2161 cttaaaatca agcacctgga aaggtagttt aagcgatagg gctattgaaa gagcgtcttt
     2221 cctatcttga tcagttcggc atccaggctc gttccagcat tccaaaaaca ccgtcgagac
     2281 ggcatgtcct tgttccacaa gtagatgagc gcagaccgcc gaatcaactc cgccagacag
     2341 ccctagagca attttcatga tttatattgt accgcgtcag tgagtttttc taaggaaatg
     2401 agtttttgga gctctgcaag ctgtgggatt tgatccaatt caccagagta ataaagcgtc
     2461 agtggttgtt taattgttgc ggaatcgcat aaaaatgcaa gccatatgtc tttatgagga
     2521 tctttggttg tttgttcaaa gattgttttt ggattgataa cttgaggttg gtaacccatg
     2581 cgtaatttaa atgttagata cccgatggta ttgaatactc caaataaatt ccgagaagta
     2641 cccgcggtga gcagtgacgg cttttttttc gtgtagtgat ccaacatcga gtagtccaga
     2701 attaatccga gcgaggcatt gtgcagtttt tggaatatag gcaactgctg gctagcctta
     2761 tatgatgagc gaacttcgtc tccaatgacg gttattattt tcccgccaat taactgatca
     2821 ttgtgtctaa tgaagatttt ttcaactttt ttcccttccc ttttttttgc actaaaccac
     2881 tcaacttttg caattggatc aaaacctcgc gccgctgttc tctcgttata cagttctaaa
     2941 aattctcgat actctttgaa agtgcatgct ccagaagaaa gttcaaactt attacgttgt
     3001 aaaaactcag gaattgtact cacaaatttt cgcgcatttt tactccgaat attttcgaga
     3061 acggatttat ctgatgtagt tggatatacc caactgactt tttgcggtcg ccaaagtgga
     3121 gtgaaatgca tcgtcggcat gattgggtgt tagtggaaat aaatttataa taaatctttt
     3181 ttatggaata aatagtagat aaaaactact gca
//
LOCUS       DAOQZP010000074         3189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_32062_length_3189_cov_5.199107, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000074 DAOQZP010000000
VERSION     DAOQZP010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32062_length_3189_cov_5.199107"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /gene="cysS"
                     /locus_tag="PKH60_04685"
     CDS             <1..1162
                     /gene="cysS"
                     /locus_tag="PKH60_04685"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNQ17368.1"
                     /translation="SMDAMGNLRPDVSCRATEHIPQQVEMVKQLEAKGFTYVIDGDGV
                     YFDTSKLDDYGKLAGLTKEKIEQLKGGARVEVVEGKRNVTDFALWKFERPGENRAMSW
                     ESPWAKRGFPGWHIECSAMAIEYLGEQFEIHTGGIDHIDIHHTNEIAQAESSTGKKPF
                     VKYWVHHNHLRVDNEKMSKSLGNFYTIDDVLARGFAPQALRLLFLGSHYRSELNFTWE
                     SLAAAQTAWDRLVKQVGEIKLSLTKNSSNSSDNFSELANTFSDQFWSAMRDDLDTPKA
                     MASFWTMLKSDLADEQKWQLLLEFDQVLGLGVKSIDQQSYRETAGSTIDRTTLPTEVQ
                     QLLSERQQAREQKNWQVSDELRDKLLQLGYRVEDSDGTQNIFYNPEALSSIG"
     gene            complement(1129..>1709)
                     /locus_tag="PKH60_04690"
     CDS             complement(1129..>1709)
                     /locus_tag="PKH60_04690"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNQ17369.1"
                     /translation="SQIDEVLSKRVYSEVDFPITTLWIFKNSIVQIPTTLIFMLIGIV
                     SPRALFTAGIFVFFFHPISLMVNWLTRKYFQYRTEDTFLFVKQGILSKKQYHLPYGVI
                     QHAIVKQDLFDRIFGLASLLIENAAQGAGIPQISSKALVYQSTAREPEFVGSSGNKIS
                     IPGLRKTDAEALKTVILQKIKDHPIDDNASGL"
     assembly_gap    1710..1809
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1810..2204)
                     /locus_tag="PKH60_04695"
     CDS             complement(<1810..2204)
                     /locus_tag="PKH60_04695"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNQ17370.1"
                     /translation="MPVTSPTKEQFPLSHKKVIKKTIGASTIWMIVLGPMYIGCLITF
                     ALYSLGPIVSLITTFVTGLLAIIIITLNYLYQKWYFDTYYYDLQQDYIVIRKDPITPR
                     EITIPYERIQDVYVDQDLYDRIFGLFDVHL"
     gene            2294..>3189
                     /locus_tag="PKH60_04700"
     CDS             2294..>3189
                     /locus_tag="PKH60_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17371.1"
                     /translation="MFTLIFALVPICVLLAGIWWIVVSQKQARQIAISFPPRSTWFGF
                     LLLIGFHLGLYDTLTAQIFPTIGLLVFGIATAVAWGIIFWQKWSPIICSVLVVMILSA
                     LLMPVRASGFIQGINFFVFFITNTLLFLRLIRRAGAQTMLRLCSDALQAGLQSISQFL
                     ALLGWLKNEKTSESQQKIVGWIKTVSLALLAALVFIILLSQADPVFAQLVRDLREQLL
                     GRFIWTVVIAIVLAVGLSMRQTQEDAPYQLRWFSRRDAIVVVAAVSMVIAGFLVVQWQ
                     YLFGSSHELLDVLNMSYSEYVR"
BASE COUNT          887 a          549 c          735 g          918 t
ORIGIN      
        1 cagtatggat gcgatgggaa atctgcggcc cgatgttagt tgcagagcga ctgagcatat
       61 accgcaacaa gttgagatgg tgaaacagct tgaagcgaag ggtttcacct acgtaattga
      121 tggcgatgga gtttattttg acacgagcaa gctggatgat tacggaaaac tggcgggact
      181 aaccaaagaa aaaattgagc aactcaaggg gggagccaga gtagaagtag tcgagggcaa
      241 gcgaaacgtc accgattttg ctctgtggaa gtttgagcgg ccgggggaga atcgagcgat
      301 gagctgggaa agtccttggg caaagcgggg atttcctggc tggcatattg agtgtagtgc
      361 gatggcgatt gagtacttag gtgaacagtt tgagattcat accggaggaa ttgaccatat
      421 tgatattcat cacaccaacg aaatcgccca agccgaatct tcgactggca aaaaaccctt
      481 cgttaaatac tgggtgcatc ataatcactt acgagttgat aacgaaaaaa tgagcaagtc
      541 gctggggaat ttttatacga ttgatgatgt tttggcgcgg ggcttcgccc cgcaagcgct
      601 gcggcttctg tttttaggtt cacactatcg tagtgaactg aattttactt gggaatcgtt
      661 ggccgcagcg caaactgctt gggatcgtct agttaagcag gtgggcgaaa taaaacttag
      721 tttgacaaaa aactcctcga attcatcaga taatttttca gaactagcaa atacattttc
      781 tgaccagttt tggtcggcaa tgcgcgatga tcttgatacg cccaaagcta tggcttcatt
      841 ttggacaatg cttaaaagcg atctggctga cgagcaaaaa tggcaattat tgcttgaatt
      901 tgatcaggtg cttggattgg gtgtgaagtc gatagatcaa caatcgtatc gagaaacagc
      961 aggttccaca attgatcgaa ctactttacc aactgaagtg cagcaattgc tttctgagcg
     1021 ccaacaagct cgagaacaaa aaaactggca agtatcagat gaactccgag ataagctact
     1081 acaacttggt tatcgtgttg aagattctga tggcacccaa aatattttct ataatcctga
     1141 agcattatca tcgattggat gatctttaat tttttgcaaa atcactgttt tcaaggcttc
     1201 agcatctgtt tttcttagtc caggaataga aattttatta ccactcgagc caacaaactc
     1261 tggttctcta gctgttgatt gatacaccag cgctttacta gaaatttgag gaattcccgc
     1321 tccttgagca gcattttcaa tcagtagaga tgctaagccg aagattcgat caaacagatc
     1381 ttgcttgaca attgcatgct gaattacacc atagggtaag tggtattgtt tttttgacag
     1441 aatgccttgt ttaacaaaca gaaacgtatc ctcggtgcga tactggaaat attttcgtgt
     1501 taaccaattt accataagcg atataggatg aaagaaaaat acaaagatac cagctgtgaa
     1561 cagtgctctc ggtgagacga taccaattag cataaaaatt agcgtcgtgg ggatttgaac
     1621 aatactgttt ttgaatatcc acaaagtagt aattggaaaa tcaacttctg agtaaactcg
     1681 tttcgagaga acctcatcaa tttgagattn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnna gatgaacatc aaataaccca aaaatgcggt catacagatc ttgatctacg
     1861 tagacatcct gaattctttc atagggaatt gtgatttctc ttggggtaat gggatctttg
     1921 cgaatgacaa tataatcttg ttgtaaatcg taataatacg tatcaaaata ccatttttga
     1981 tagaggtaat tgagggttat tatgatgatc gcgagcagac cagtaacaaa tgtggtaatc
     2041 agtgagacga tgggaccaag tgaatagagg gcaaaagtaa ttaagcaacc aatatacatg
     2101 ggtccgagca cgatcatcca aatcgtactt gccccgatgg ttttcttaat cacttttttg
     2161 tgactgagag gaaactgttc cttagtgggt gatgtaactg gcataaattt tcaattatga
     2221 tactaattta gtatagcagt gagcttgttg aagggggaag gtgcgttata atgaatagac
     2281 tggagggttc gatatgttta ctctcatttt tgctctagta ccaatttgtg tgttgttggc
     2341 agggatttgg tggattgttg ttagtcaaaa gcaagcgcga cagattgcaa tctcgtttcc
     2401 tccaagatca acttggtttg gttttttgtt gctgattggt tttcacctcg gtttgtatga
     2461 cacactaaca gctcaaattt ttccgaccat tggtttatta gtttttggaa tcgcaaccgc
     2521 agttgcttgg ggaataatct tttggcaaaa atggtcgcca attatttgtt cggtactcgt
     2581 agtaatgatt ctaagtgcgc tgctgatgcc agtaagagcc agtggtttta ttcagggcat
     2641 caactttttt gtgtttttta taacaaacac acttcttttt ttacgactga tacgaagagc
     2701 aggggcacaa actatgctcc gattgtgtag tgacgcgctt caggctggtc tacagagcat
     2761 tagccaattt ttggctttac ttggttggtt aaaaaacgaa aaaaccagtg agagccaaca
     2821 aaaaattgtt ggctggatta aaacagttag cttagcatta ctcgctgctt tggtatttat
     2881 tattctatta agtcaggcgg atccagtttt tgctcagtta gtcagagatt tgcgggagca
     2941 attgcttgga cgatttattt ggacagtggt cattgccatt gtgttggcag ttggcctttc
     3001 gatgcgacag acgcaggagg atgcaccgta ccaactaaga tggttttctc gccgagatgc
     3061 gattgtggtc gttgcagcag taagcatggt gattgctggt ttcttggtcg tgcagtggca
     3121 gtatttattt ggtagttccc acgaattact ggatgtatta aacatgagct atagcgagta
     3181 tgtgcgcaa
//
LOCUS       DAOQZP010000075         3170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_32338_length_3170_cov_3.948957, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000075 DAOQZP010000000
VERSION     DAOQZP010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32338_length_3170_cov_3.948957"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /locus_tag="PKH60_04705"
     CDS             <1..1495
                     /locus_tag="PKH60_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17372.1"
                     /translation="GESDYLKSITQGDLLFVISQSGLPVITDGLDKTALLEKTYAQFV
                     SNFKQLVSEEIVKKIESSNQQKYGIFPLAEFGLKNREKTLNSRREMAISIGTQTLGFG
                     YYEPNIRSDYPNAGKLRAEEVASDGSIEVVYRELTDDEKDKAFEAGKAAELFGFNWYI
                     KTCSHMFDDDVTAKAALTFQRVAISERYRSNYRNSRAELDGKYRRGISQVGGLLPRPL
                     SYFGVEGISLQRILTRPDLYETADFSRIGDEAGGQIKAYKELWEWSVKMYKAMSIGAS
                     TVMGKNILQVGQIKTVKTVVAGVQDLEPDASAVRELATFATKVVTYWLNNVITTEGEM
                     RSTDSSFFGVDESYWKQLKSPIDSSITTSIRTPLMTMFRLQPAEMHQLALQVSKAKNQ
                     KEEFTKEFMKRFIKICLIEDLMRSAKDTIDPEDYVTLCWVLTGSVSQWATLKYSIKDQ
                     GNPFTSDTGDIFKFTEVDVAHMCLEAGLALPPKVFEGLREAGGISGA"
     gene            complement(1715..2194)
                     /locus_tag="PKH60_04710"
     CDS             complement(1715..2194)
                     /locus_tag="PKH60_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17373.1"
                     /translation="MPMGLSGRNIAKLGAGAAGTALATPFTAGAGALIGSKMGYGAKK
                     GAMVGAALPAGIVAGIPLAKRGVSVINKGVSGIQNLGKSYTTISNVAGSVGTIAAAGP
                     AMKEAKSFREKVGIGMNTATEVARRVSPSRADEIAEATRAASGESSSASRDTGDSEV"
     gene            complement(2219..>3170)
                     /locus_tag="PKH60_04715"
     CDS             complement(2219..>3170)
                     /locus_tag="PKH60_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17374.1"
                     /translation="TQSYVADVLKSAHIATPAYAQGLGFASLDPVLNLWKTFRNIAYM
                     FFVLIFVIVGFLIMFRQKMGQTAITAQQAIPQIIMSLVLVTFSYAIAGLLIDLMYVLM
                     ALIVGVFASFFPQDIFGYNIFELGGTMFQGAVNLRRNKDFISGFLESLHLGGTANQVV
                     SIISGLTLTIILAVAMLIGIIRLFFELLKSYATIIIAVVMAPLLLMMGAIPGKSNAFG
                     SWIKTLIGNLSPFPVILVVLVMYYAFTNGAINASSGGFMPPFLLSAGNGIGESIIALM
                     SLAIILALPDIVKKIKDGIAPKDPLAELVTGKGLENFKAG"
BASE COUNT          982 a          646 c          709 g          833 t
ORIGIN      
        1 gggagagtcc gactacttga aaagtattac acaaggagat ttgttatttg taattagcca
       61 gtcggggctc ccagtgatta ctgatggttt ggataaaaca gcactcttgg aaaaaactta
      121 cgcacagttt gtctcaaact tcaaacaact agtatcagaa gaaattgtta agaagataga
      181 atccagcaac caacagaaat atggaatttt tccgctggcg gaatttggtt taaagaaccg
      241 agaaaaaacg ctcaactcaa gaagagagat ggcgatttct atcggcaccc aaactcttgg
      301 atttgggtac tatgagccaa atatcaggag tgattatcca aatgcaggca aacttcgtgc
      361 agaagaagta gcgagtgatg gctcaataga agtggtctac agggagctta ctgacgatga
      421 gaaagataag gcatttgagg ctggaaaggc agcggagtta tttggtttta attggtatat
      481 taaaacatgt agtcacatgt ttgatgatga tgtaaccgct aaggccgcgc taacatttca
      541 aagagttgcg attagtgaga gatatcgaag caactataga aatagtagag ctgagcttga
      601 tggtaagtat aggaggggga ttagtcaggt gggcggatta ttgcccagac cactgtcata
      661 ttttggagta gagggtattt cgctccagag aatattgact cgaccagatt tgtacgagac
      721 ggctgacttc tcacggattg gagatgaggc tggaggtcaa attaaggcat ataaagaact
      781 ttgggaatgg tcggttaaaa tgtataaagc catgtctatt ggcgcatcta cggtcatggg
      841 taaaaacata ttgcaggttg ggcagattaa gaccgttaaa acagttgttg ctggtgttca
      901 agatttagag cccgatgcaa gtgctgtcag agaacttgca acatttgcaa caaaagttgt
      961 gacttattgg ctgaacaatg tgattacgac agagggagaa atgcgatcta ctgactcctc
     1021 tttttttggt gtagatgaat catactggaa acaactgaaa agcccgattg attccagcat
     1081 aacgaccagc attcgaaccc cgttaatgac tatgtttaga ttacaaccag ctgagatgca
     1141 ccaacttgca ttgcaggtaa gtaaagccaa aaaccaaaaa gaagaattta ctaaagaatt
     1201 catgaagcgg tttataaaga tttgtttaat tgaagatctt atgagatcgg caaaagacac
     1261 aattgatccc gaggattatg ttacactatg ttgggttttg accggttctg tctcgcaatg
     1321 ggcgacactt aaatactcaa ttaaggatca aggcaatcca tttacaagcg acaccggaga
     1381 tatctttaag tttactgagg tggatgttgc gcatatgtgt ttggaggcag gattggcttt
     1441 accaccgaaa gtgtttgaag gcttgagaga ggctggtgga attagtggag catagaaaaa
     1501 gaaaattcaa tcacttttta ccaaaagggc atatagtgca agagcaacct gcacttggga
     1561 tgtaagtagt gcattttaat ataatctcta tcatcgttac tgtgacgttg atcatctgaa
     1621 tagatattaa gaggtttaaa aagcaacaaa atatggattg acatctattg tttgcacaga
     1681 cccaagacac catgtgttta tcacaaagaa aggtttaaac ttcagagtcg cccgtatctc
     1741 ttgaggcaga tgaagactca ccactagctg ctcgagttgc ctcggcaatt tcatctgctc
     1801 tgctcggaga gactctcctt gctacttcgg ttgcggtgtt catgccaatg ccaacctttt
     1861 ctctaaagct ctttgcttct ttcatggcag gtccagctgc tgctattgtg cctactgacc
     1921 cagcaacatt agaaatagtt gtgtaggatt tgccgagatt ttgtatacca ctcacgcctt
     1981 tatttataac actaacacca cgctttgcca atggtatacc tgcaacaatt ccggcgggga
     2041 gagctgcgcc aaccatagct cccttctttg caccataacc cattttagaa cctatcaatg
     2101 cgcctgctcc agccgtaaaa ggagttgcta gggcagtgcc agcagcgccc gctcctaact
     2161 tggcaatatt cctcccactt aaccccatcg gcatcttgcc ccaaggagtc ttgcctgatc
     2221 accctgcctt gaagttttca agacctttgc cggtcaccaa ttccgccaac ggatctttgg
     2281 gtgcgatgcc atctttaatt ttctttacaa tatctggcaa agccaaaata atcgccaaac
     2341 tcatgagcgc aattatgctt tcaccaatcc cgttacccgc gctcagtaga aatgggggca
     2401 taaaccctcc actgctggca ttaattgcgc cattggtaaa cgcataatac atcaccaaaa
     2461 ctactaaaat tactgggaaa ggcgaaagat tcccaatcag tgtttttatc caggatccaa
     2521 aagcgttact tttacctggg atagctccca tcatgagtag caagggcgcc atcaccaccg
     2581 caataataat ggtggcatat gattttagta attcaaaaaa taacctaatt atgccaatta
     2641 acatcgcgac ggctaaaatt attgtgagcg ttaagccact aataatactc accacctgat
     2701 tggccgtacc accaagatgt aaactctcaa gaaaaccaga aataaagtcc ttgtttcgcc
     2761 ggaggttaac tgctccctga aacatggttc ctccaagctc aaaaatattg tagccaaaga
     2821 tatcttgggg aaaaaatgag gcgaaaacac caacgatcaa ggccatgaga acgtacatta
     2881 ggtcaatcaa taaccctgca atggcatagg aaaacgtcac tagtactaac gacatgatga
     2941 tctgaggaat ggcttgctga gctgtaatag cggtttgacc cattttttgc cgaaacatga
     3001 tgagaaagcc cacaattaca aagatcaaaa caaaaaacat ataggcgata ttgcgaaagg
     3061 ttttccagag attgagcact gggtctaggg aggcaaaacc aagtccttgg gcatatgctg
     3121 gagtggcgat gtgagctgac ttgagtacgt ctgccacata cgactgagta
//
LOCUS       DAOQZP010000076         3163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_32458_length_3163_cov_3.888031, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000076 DAOQZP010000000
VERSION     DAOQZP010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32458_length_3163_cov_3.888031"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1865
                     /locus_tag="PKH60_04720"
     CDS             <1..1865
                     /locus_tag="PKH60_04720"
                     /inference="COORDINATES: protein motif:HMM:NF021251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure C-terminal
                     domain-containing protein"
                     /protein_id="HNQ17375.1"
                     /translation="TSQRAAAELQLKEVEQSRIATGELDSQKQTEAVNKIYESNQIIN
                     SYGDQMMTAAEKAHELARQVTAVGYAGNDMRIGLEGALKKIHELSGVDVSSFGRSIDD
                     RIEGLKIKIKTLQDNPLASSGEVTARARYAEQTSRLDRAANAAAAGAAATTPAAMERI
                     AEIDQENRMKSQQELELTLQEKRLEDQRRELKKSKSGGGAPRSFTKQFDTMERDMRQF
                     QQRSEEAWSQYWIEVFETSGMPYEAAKEKTDNWYRDQIFSVTEASRKISDKMKELQED
                     IATGEASKRGNRPETEAHLAKLQEELNKAKEMEAPLLKIVDVWLLLRQAQDEYHKLDV
                     PAQWTEEQLAISKLVGTLEEQAYWENTLNKIKAEQSKQDKDPAIQALIDQRTALENER
                     RSALAGDDFGAGFMVTARDAFRELGGWGQIGADAFTMLSDSISSTADTLTEFLTTGKI
                     EFADFANSIIKDMIRIITQRLIMNAVFGVINLISPLSGGAIGGGTALGGGVSGTGGFL
                     SQVVTKHNGLGTVSKMVPDSYFFNAPRLHDGLAADEYPAILQKGEAVIPKNHSLANTS
                     NITVNITQNGDSGNASDAQILAKIVAKAVDEKIQQSFRKQMRNGGILDSHKRVA"
     gene            1867..2370
                     /locus_tag="PKH60_04725"
     CDS             1867..2370
                     /locus_tag="PKH60_04725"
                     /inference="COORDINATES: protein motif:HMM:NF017726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNQ17376.1"
                     /translation="MADDFSIEPSYAIQMDENVEVNKADFGDGYSQRTVSGLNNIKTI
                     WKLVWEGISTANANSLIALFRGRRGVDVILWTPKRAVTTASKVVYETNHYYCKLTHIS
                     ETGNAPGNTTYWGSSSGATATWTSGMEYYDGTSTEQKWICSSWSRSFNSNEFDTVSAT
                     FERVFDL"
     gene            2380..>3163
                     /locus_tag="PKH60_04730"
     CDS             2380..>3163
                     /locus_tag="PKH60_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HNQ17377.1"
                     /translation="MADHPFATDVYKPDLGEEVELFILDPTAIDSSLSVMYFCSSVKY
                     NSAVKWKNAAGDGWVDYSPIPIEAEGFESTGQGTLPRPKIRINNVGALGALQGPLFTT
                     MRDYNDLLGAKITRYKTLGKYLYGNTTQDYDAHFPPDVYLINQKTAQNPIYVEWELAP
                     YFDCQGIKLPRRLILKDICQLRYRYWNSTSSAWVYDDTENACPLQDRLLTLDGNEFDF
                     SSAGTWELSGGWTISDGKLNHASGSGGGANDTAYIYTLLKPGV"
BASE COUNT         1008 a          569 c          746 g          840 t
ORIGIN      
        1 ccacgtcaca aagggctgct gctgaattac aattaaaaga agtagagcaa tcaagaattg
       61 ctactggaga attagactct caaaaacaaa cagaagcagt caataagata tacgaatcaa
      121 atcaaattat taattcgtat ggtgatcaaa tgatgactgc tgctgaaaaa gctcatgaat
      181 tagcaaggca agttacggca gttggatatg ctggtaatga catgagaatt ggattggaag
      241 gtgcattaaa aaagattcat gaattaagcg gtgttgatgt ttcgagtttt ggtcgatcta
      301 ttgacgatag aatagagggt ttaaaaatca aaataaaaac attgcaagac aatcctcttg
      361 catcgagtgg agaagtaact gcaagagcga gatatgcaga acagacatct aggcttgatc
      421 gagctgccaa tgccgcagca gcaggtgcgg cagcaacaac tcctgccgcc atggagagaa
      481 ttgcggaaat agaccaagaa aatcgtatga aaagccaaca agagctagaa ttgacgttgc
      541 aagagaaacg acttgaggac cagagaagag aattgaagaa atccaaaagt ggtggtggag
      601 caccacgatc attcacaaag cagtttgata ctatggaacg tgacatgcgt caattccagc
      661 aaagatctga agaggcgtgg tcccaatatt ggattgaggt atttgaaact tctggaatgc
      721 catatgaagc cgcaaaagag aaaacggata attggtacag agaccagatc ttctcagtca
      781 cagaagcaag tcgtaaaatt tcagataaga tgaaagaact ccaggaagac attgctactg
      841 gagaagcatc taaacgtggt aatcgtccag agactgaagc tcatttggcc aaattacaag
      901 aggagcttaa caaggcaaaa gaaatggaag ctccattact taaaattgtt gatgtttggc
      961 ttttattaag acaagctcaa gatgaatatc ataaattaga tgtccctgcc caatggaccg
     1021 aagaacaact tgcgatttct aaacttgttg gcactcttga agaacaggct tattgggaaa
     1081 atacgcttaa taaaattaaa gccgaacaat ctaaacaaga taaagatcca gcaattcaag
     1141 ctctaattga ccaaagaact gcacttgaaa atgaaagaag atctgctctt gctggagacg
     1201 attttggtgc tggttttatg gttacagctc gtgatgcatt tcgagagctt ggtggttggg
     1261 gtcaaattgg tgccgacgca tttacaatgc tctcagattc aatatcaagt acggcagaca
     1321 cacttactga attcttaaca acaggaaaga ttgaatttgc tgatttcgcc aatagcatta
     1381 tcaaagacat gattcgaatt attacacagc gacttataat gaatgcagta tttggcgtta
     1441 ttaatttgat tagtccatta agtggtggtg caattggtgg aggaactgct ttgggtggtg
     1501 gagtaagtgg gactggtgga tttttgtctc aagttgtaac aaaacataat ggattgggaa
     1561 cagtaagtaa gatggttcct gattcgtact tcttcaatgc tcctcgtctt catgatggtc
     1621 ttgctgccga tgagtatcct gcgattcttc agaagggtga agcagttatc ccgaagaatc
     1681 attcgttggc taacacgagc aatattaccg ttaatatcac tcagaatgga gacagtggga
     1741 atgcctcaga cgctcaaata ctggcaaaaa tagttgctaa ggctgttgat gaaaagatcc
     1801 agcagagttt tcgaaaacag atgcgtaatg gtggaatctt agattcacat aagagggttg
     1861 cataaaatgg cagacgactt ctcaatagaa cctagctatg ctattcaaat ggatgagaat
     1921 gtagaggtta acaaggctga ctttggagac ggctattctc aaagaacggt ttctggatta
     1981 aataatatca agacgatttg gaaactggta tgggaaggta tctctacagc caatgccaat
     2041 tcattaatag ccttgtttcg agggcgtaga ggagtcgatg ttattctgtg gactcctaaa
     2101 agagcagtga ctacagcaag taaggtagtc tatgaaacca atcattatta ctgcaagcta
     2161 actcatattt ccgagaccgg gaatgctcca ggaaatacga catactgggg aagttctagt
     2221 ggagcaaccg caacgtggac tagcggcatg gaatattacg atggcacatc cacagaacag
     2281 aagtggattt gttctagttg gtcgagatca tttaactcca atgaatttga tacagtgagt
     2341 gctacttttg agagagtctt tgatctttaa ggaggcatag tggcagacca tccttttgca
     2401 acagatgttt ataaacctga tcttggggaa gaggtcgagc tttttattct tgatccgacc
     2461 gcaatagact cttctctctc agtgatgtat ttttgttcat ctgtaaaata caattctgct
     2521 gtgaaatgga agaatgctgc cggtgatgga tgggtggatt attctcctat tcctatcgaa
     2581 gcagaaggtt ttgagtctac gggccaaggc acattgccga gacctaagat cagaatcaat
     2641 aatgttggtg ctttgggtgc attacagggg cctctcttca cgaccatgcg ggattacaac
     2701 gaccttcttg gagccaagat cactagatat aaaactcttg gtaaatatct ctatggaaat
     2761 acaactcaag attacgatgc ccacttccct cctgatgttt atttgatcaa tcaaaagaca
     2821 gcacaaaacc ccatatatgt tgaatgggag ttggcccctt actttgactg tcaggggatt
     2881 aaattgccta gacgattgat tcttaaagac atatgccagt tgagataccg ttattggaat
     2941 tctactagct cagcatgggt ctatgatgat acagaaaatg cctgtccatt acaggatagg
     3001 ttattgacgc tggacggtaa cgagtttgat ttttcatcag ctggaacttg ggagctttca
     3061 ggtggctgga caatcagcga tggcaagctc aatcacgctt ccggttctgg tgggggggcc
     3121 aatgataccg catatatcta taccctcctc aagccggggg tga
//
LOCUS       DAOQZP010000077         3111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_33288_length_3111_cov_4.330170, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000077 DAOQZP010000000
VERSION     DAOQZP010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33288_length_3111_cov_4.330170"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..1334
                     /locus_tag="PKH60_04735"
     CDS             123..1334
                     /locus_tag="PKH60_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008104708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNQ17378.1"
                     /translation="MIHTDYSPPAEILNKYAQVLVHFALGQTEGIKSGEVVQCLVPDV
                     AKPLALALQNTLLKAGAHVLMRLLPTGFDSDYYSLASKEQLTFFPEQHLQTRADLIDH
                     SIGILADPFPEELKNVDPDSIMLARNTQKPYRDWLMDKEIQGKFTWTLALWGVDAKAK
                     IVGLSLEEYWQQIIKACFLDQADPIAEWRKVKRLQQEFRDKLNALSIESVQITGPDVD
                     LKIQLGANRQWKGGADRNIPSFELFTSPDWRGTQGHIKFNQPLYRYGNILEGIELHFQ
                     NGVISKASATKGNQLLQTMIKSPNANKLGEFSLTDSRLSKITHFMAETLYDENIGGPE
                     GNTHVAIGMAYRDCLRGDKTTLSNAEWENLGFNDSPEHTDIISTTNRTVTATLTDGST
                     KVIYANGQFTV"
     gene            complement(1654..2712)
                     /locus_tag="PKH60_04740"
     CDS             complement(1654..2712)
                     /locus_tag="PKH60_04740"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HNQ17379.1"
                     /translation="MNYEEIITLLGDEAESLLSHTCEKIPKDLIISPGPESVLTVLRD
                     SNRSSRVIANLQRLYHHGRLAGTGYLSIFPVDQGLEHTAAYSFYQQPHYFDPQAILEM
                     AIAGGCSGVASTFGVLSLVSKKYQSKIPFIVKINHSEHLTLPELADQLIFTSVDQAAT
                     LGAAAIGATVYFGSNTSHKQIEQISRLFERAHQLGMATILWCYPRNDHYKKDSQSYEE
                     AADVTAQAIHLGVSMGADIVKQKMPTSLRAFQALNFSKYDDQMYDQLLTDHPIDLVRY
                     QVLHAYSGRIGLINSGGESLGEDNLRAAVRQAVINKRAGGHGLIMGRKVFKSDWDTGV
                     KLLRAVQDVYLEPKITIA"
     gene            2777..>3111
                     /locus_tag="PKH60_04745"
     CDS             2777..>3111
                     /locus_tag="PKH60_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan phosphorylase"
                     /protein_id="HNQ17380.1"
                     /translation="MQPANPTVLETPIAYLSAEFGFDHNLPIYAGGLGILAGDTMKQA
                     ADDHLPFVGVGLLYRGQYMKQTITPNGEQLEEQWYFDPVTVGLEHVYKDGDQPVFITV
                     QIGNESVWL"
BASE COUNT          897 a          647 c          635 g          932 t
ORIGIN      
        1 atcagtaaag aatagcttat cccatcgtca caaggcactc gagttagcta aaatctacct
       61 tcaaaatcac ccagaatttc ttagcttgta gtttcaaaga atcatttgta caatcatgcc
      121 tcatgattca tactgactat tctcctccag cggaaattct taataaatat gcccaagtat
      181 tagtccactt tgcgcttgga caaacagagg gcataaaatc aggcgaagta gtgcaatgcc
      241 tcgtgccaga cgttgctaag cccctggcgt tggctctaca aaatacactt ttaaaagctg
      301 gggcacatgt gttaatgagg ttgttgccta caggattcga tagcgactat tattccctgg
      361 cttcaaaaga acaattgact ttttttccag aacaacacct acaaaccaga gcagatttga
      421 tcgatcactc aattggaatc ttagctgatc catttcccga agaattaaaa aacgtcgatc
      481 cagactccat tatgttggct cgaaatactc aaaaacccta tcgtgattgg ttgatggata
      541 aagaaatcca aggtaaattt acttggactc tggctctctg gggagttgat gccaaggcga
      601 agatcgttgg tttaagttta gaagaatatt ggcagcaaat aattaaagct tgttttcttg
      661 atcaggctga tcctattgcg gagtggcgaa aagtgaaacg attgcaacaa gaatttcggg
      721 ataagctcaa tgctctttca attgagtccg ttcagattac tggcccagat gttgatttaa
      781 aaattcaact tggcgccaat cgccagtgga aaggtggtgc tgaccgaaat attcccagtt
      841 ttgaactatt taccagtccc gattggcgcg gtactcaggg tcatatcaaa ttcaatcaac
      901 cattgtatcg ctacggcaat attcttgaag gcattgaact ccacttccaa aatggtgtaa
      961 tttccaaagc atcggctacc aaaggcaatc aattactcca aaccatgatt aagagtccga
     1021 atgccaataa gttaggagaa ttttcattga ctgatagtag gttatcaaaa attactcact
     1081 tcatggcaga aacactctac gatgaaaata ttggtggccc cgaaggtaat acccatgtgg
     1141 cgattggtat ggcctaccgt gactgcttga gaggtgataa aacaacgcta tctaatgctg
     1201 agtgggagaa tcttggtttt aatgattcgc cagagcacac cgatattatt agcactacca
     1261 atcgtacggt aactgccacg ctaaccgatg gttcaacaaa agtgatttac gccaatggtc
     1321 agtttactgt ttagtcacac ccgctgcatt taatttgata gtttacagta aagcatcgct
     1381 tcgtctggag gcttgcttta gtgtaaacca tgtttttaaa agtgatgtgt tgtttcaaat
     1441 taaattattt ctagtttttt cgaatttgtc ctttaggctt cgacgttcgg aaatttgctc
     1501 ttagagtaaa accaagccga tcaaacatcg cacatgtctt taattttatg gtttagtata
     1561 aaccactact ttgcctggag gaatgcttta gtgtaaacca tgtttttgaa agtgaggcat
     1621 cgtttttcaa actatgtttt tgtgtaatca aaactaggcg atggtaatct tgggttcaag
     1681 atacacatcc tgaactgccc gtagtaattt cactccagta tcccaatcgc tcttaaacac
     1741 ctttctcccc atgattagac catgaccccc tgctcgcttg ttaataactg cctgtcgcac
     1801 agctgctcgc aaattatcct ctcccaaact ttcacctccc gagttaatca acccgattct
     1861 tcctgaatag gcatgtagta cttgatagcg tactaaatca attgggtgat cggtgagtaa
     1921 ctgatcatac atttgatcgt catattttga aaagtttaat gcttgaaatg ctctgagtga
     1981 agttggcatc ttttgtttca caatatccgc ccccattgat acacccaaat gaatggcttg
     2041 agctgtaaca tctgccgctt cctcatacga ttggctatct tttttatagt gatcatttct
     2101 gggataacac cataaaatag ttgccatacc cagttgatgt gctctttcaa atagacgaga
     2161 gatctgttca atttgcttat gtgatgtatt ggatccaaaa tataccgtag cgccaatagc
     2221 tgcagctcca agagtcgcag cttgatcgac actagtaaaa attagttgat cggccaactc
     2281 tggtagtgtg aggtgctcac tatgattaat ttttactata aatggaattt tgctttgata
     2341 tttctttgaa actagcgaaa gcactccaaa cgtacttgct actccactac agccacccgc
     2401 aattgccatc tcaagtattg cctgaggatc aaaataatgt ggttgttgat aaaacgagta
     2461 agcagcagta tgttccaaac cttgatctac gggaaatatt gagagataac cggtgccagc
     2521 tagtctaccg tgatgataca agcgttggag attggcaatg accctgctcg accgatttga
     2581 atcacgtaag actgtaagga cgctttctgg cccaggggaa atgattagat ctttgggaat
     2641 cttttcacag gtgtggctaa gtagtgattc cgcttcatca cctaataatg ttatgatctc
     2701 ttcatagttc atagattcta tagtagcaaa agaatgactc gattgactag taggatcgtg
     2761 atacactaga aattcaatgc agcctgccaa cccaaccgta ctggaaactc cgattgctta
     2821 cttgagtgct gagtttggct ttgatcataa tctccctatt tatgctggag gactcggcat
     2881 acttgcagga gataccatga agcaggctgc agacgaccat ttgccgtttg ttggtgttgg
     2941 tttgctctat cgtggtcagt atatgaagca aaccattact ccgaatggcg aacagttaga
     3001 ggagcagtgg tattttgatc cagtaacagt tgggcttgag catgtttaca aggacggaga
     3061 tcaacccgtc tttattacag ttcaaatcgg caatgaatca gtttggctga g
//
LOCUS       DAOQZP010000078         3094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_33566_length_3094_cov_4.540967, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000078 DAOQZP010000000
VERSION     DAOQZP010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33566_length_3094_cov_4.540967"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..930)
                     /locus_tag="PKH60_04750"
     CDS             complement(7..930)
                     /locus_tag="PKH60_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNQ17381.1"
                     /translation="MIKILYRSLKDSKIHTSKKSRRGIWVRVEDPTELELENLIEDYD
                     LDEAIVKDALDPYEVPRIEKEDGAVYCIIRLPTASQTTIVTIPLLIVITPLVVITVSK
                     NKLELLDTLILEKLAPVITTQKTKLLMQLLQLINAEFEKQLFRLAKDISLHTTDTEKI
                     ENARVIQFVVYETQYNTYLATLNPMKLVLEKLRSNKFLALHEDDNDLIEDLLWNADQL
                     IQMSLFGIKQVTNIREAYSTIVSNNLNKTVKVLTSVTVILTIPTIISSFFGMNVQVPL
                     SDHVFGFLLIAIFTIIAVVFIYYLFHKKDLL"
     gene            complement(948..2915)
                     /locus_tag="PKH60_04755"
     CDS             complement(948..2915)
                     /locus_tag="PKH60_04755"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNQ17382.1"
                     /translation="MKKSFMMFNFFSVFSLLLASAFFYPSKICAKSYTIDSVVIDANL
                     QRDGSMSVTEQRKFNFDGDFTFANQEILKNPDQSQNTGRTESFEITDFSICDELDCYT
                     ALLGQEKLAADEVRPSEKFYLNDLDDAYFIKWFYRSNTSKTFTISYIVRNAITLHSDT
                     AEIYWQWLGNKSEIPQNSVKVTLNFPEAIPDGDITAWLHGPLSGVVAIPDAKTVTFTV
                     DQVPALTFVEGRVVVPLSTFDAGAKGSLSKQQIIDQELQFVQQTEAAKQKSVWLYIGQ
                     ALLIFLIGGLALVLQIWMIITFLRYGKDEPLPKINLSGTLWEPPSDIDPAQIEQLLSG
                     GETTTSKAFTASVLSLVQNKLYRLIRSEQKEGLLFKDYRYYLVPSNRTDTSLSSIQNA
                     VYSFLRKIPLKTVEVEGKKQDALPLKDISNWCLSHRAESHSFFTNLKTIVLEENLKEN
                     FFDEASKKHRWKYSLGIVAGIGLFIFSIIMMSLLSEASNLGDFTWLMNVAAVTAFFEG
                     PLAVLLFLTGSIFYSAGLKRTKTGGLETAGWKAFKKHLNDYAKTKESPIDSIILWEKY
                     LVYGTILGVSAKALSQLPVNFSAADQKYLAANWGSADILTTGGISHLSAAIGSISAAA
                     TASYGASGVGSSGGASGGGGGGGGGGGVGAG"
     gene            3022..3092
                     /locus_tag="PKH60_04760"
     tRNA            3022..3092
                     /locus_tag="PKH60_04760"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3054..3056,aa:Gln,seq:ttg)
BASE COUNT          926 a          603 c          605 g          960 t
ORIGIN      
        1 ataaatttat aataaatctt ttttatggaa taaatagtag ataaaaacta ctgcaattat
       61 agtaaaaatg gcaatcaata aaaatccaaa tacatgatcg gataagggta cttgcacatt
      121 cataccaaaa aaacttgaaa ttattgttgg tatggttaat ataactgtga ctgatgtgag
      181 caccttaact gttttgttta aattatttga aacaatggtg gagtatgcct cgcgaatgtt
      241 ggttacttgt tttataccaa acaaactcat ttgaatgagc tgatctgcat tccacaacaa
      301 gtcttcgatt aaatcgttat catcttcgtg taaggccaaa aatttatttg atctgagttt
      361 ttctagtact aatttcattg gattgagtgt agcgagatag gtattatatt gggtctcata
      421 caccacaaac tgaatgacgc gagcgttttc aattttttcc gtgtcggtcg tatgcaaact
      481 aatatctttc gccaagcgaa ataactgttt ctcgaactca gcattgatta gttgcaacaa
      541 ctgcatgagc aattttgttt tttgcgttgt aatgacagga gctagcttct ctagaatcag
      601 tgtgtcaagt agttctaatt tattctttga tactgtaatg acaacgaggg gagtaataac
      661 gataagtagt gggatagtca cgatggtcgt ttgactagct gtgggaagtc gaataatgca
      721 gtagacagct ccatcctctt tttcaattct aggaacttca tagggatcca gggcgtcttt
      781 tacaattgct tcatctaaat cataatcttc aattaagttt tctaactcca attcagttgg
      841 atcttctacc cgaacccaaa taccacgtcg ggatttttta gaagtgtgga ttttactatc
      901 tttgaggctt ctgtagagga tttttatcat gagcagtgcg tagtatacta gccagctccc
      961 acaccgccac ccccacctcc accaccccct cccccactcg ctccacccga tgaacctact
     1021 ccagaagctc catagctggc tgtagctgct gcgcttatcg atccgatggc tgcagaaaga
     1081 tgagatattc caccggtagt aagtatgtct gcactgcccc aattagcagc taggtatttc
     1141 tgatctgcgg cagaaaaatt aacaggtaat tgtgataatg ctttggcaga tacaccaaga
     1201 attgttccat ataccaagta tttttcccag agaataatag aatcgatagg cgactctttg
     1261 gttttcgcgt aatcatttaa atgttttttg aatgctttcc aacccgccgt ttcaagtcca
     1321 ccagttttgg ttcgttttaa acctgctgaa tagaaaatac taccagtaag aaaaagtagt
     1381 actgcgagtg gaccctcaaa aaatgcggta actgccgcga cattcattaa ccaagtaaag
     1441 tcgcccaaat ttgaagcctc actaagtaag ctcatcataa ttattgaaaa aataaatagt
     1501 ccgattccag caactatccc aagcgaatat ttccaacgat gtttttttga tgcttcatca
     1561 aagaagtttt ccttaagatt ttcttcaagc acaattgttt ttaagttggt aaaaaagcta
     1621 tgactttcag ctcgatgcga taaacaccaa ttactaatgt ctttcagtgg taaagcatct
     1681 tgcttttttc cttcaacctc aactgtttta agaggaattt tgcgtaagaa tgaatagact
     1741 gcattttgaa ttgaagaaag cgatgtgtct gttcggtttg aaggaaccag atagtatctg
     1801 taatctttga acaacaagcc ttctttttgc tcacttctga ttaagcgata gagtttattt
     1861 tggactaaag acaaaacgct cgctgtaaac gcttttgatg ttgtagtttc tcccccagag
     1921 agtagttgtt caatttgagc agggtcaata tcgcttggtg gttcccacag ggttcccgac
     1981 agatttatct ttggtagcgg ttcatctttg ccatatctaa ggaaggtgat aatcatccag
     2041 atttgtagta ctaacgctaa tccaccaatg aggaaaataa gcagagcctg gccaatataa
     2101 agccagaccg atttctgttt ggcagcttcg gtttgttgaa cgaattgtaa ttcttgatca
     2161 ataatttgct gcttagaaag agaacctttg gctccagcat caaaggtact caatggcacc
     2221 actaccctac cctcaacaaa agttaacgct ggtacttggt caactgtaaa agtaacagtt
     2281 tttgcgtctg gaatagctac caccccactg agggggccat gaagccaggc cgtaatatct
     2341 ccatcgggaa tcgcctcggg aaagttgagg gtgactttta cagaattctg cggaatttcg
     2401 gatttatttc ccaaccattg ccaatagatc tcagcagtat ctgaatgcaa ggtaatagca
     2461 ttgcggacga tgtatgaaat agtaaaagtt ttagatgtat tgctacggta gaaccatttg
     2521 atgaagtatg catcatctag atcattaaga tagaattttt ccgatggtct aacttcgtct
     2581 gcggctagtt tttcttgacc cagcagggct gtgtaacaat caagttcgtc gcagattgaa
     2641 aagtcagtaa tttcaaatga ttcagttctg cctgtattct gactttgatc tggattttta
     2701 agaatttcct gattggcaaa agtgaaatcg ccatcaaaat taaattttct ttgttcagtg
     2761 acggacatgg agccatctcg ttgtagattg gcatctatca ctacgctatc aatagtatag
     2821 gattttgcac aaattttaga tggatagaag aatgcagaag ctaaaagcaa gctaaataca
     2881 gaaaaaaaat taaacatcat gaaagatttt ttcatagtta aactctagta tagaccatca
     2941 actgacgtga cttcgagccc cactggcaac tggaggatga tttgttacaa tatgacctgc
     3001 gcaaagtgaa aatttacaca ttgccccgtg gtctaatggc aggacagcag cctttgaagc
     3061 tgtttatcta ggttcgaatc ctagcggggc atca
//
LOCUS       DAOQZP010000079         3065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_34007_length_3065_cov_5.358140, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000079 DAOQZP010000000
VERSION     DAOQZP010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34007_length_3065_cov_5.358140"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="PKH60_04765"
     CDS             <1..974
                     /locus_tag="PKH60_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17383.1"
                     /translation="LILKNGTSAIILTVDAMNFGLLAEEEQDAIMYAYAGLLNSLNYP
                     IQIVVQSKTKDVTGYLRKLTEQEEKTSNETMRKRIAQYRLFVANLIHERNVLDKRFYV
                     VIPATSIEMGFLGAQTFVPGKEDFNISDVERSVVIEKATNLLEPKRDHLISQFARIGL
                     YSRQLNTQEIIQLFYVNYNPESTEGQEIAESSAYTSGLVHADMGTAYGDVAVSPVTNA
                     YESNTDPQPQSAPVSEAQPAPSPSQSPSALNQVNQEQIVWSQPQQSIQEPTQANVQDP
                     FHSPVQTQAQSGYQPPSSNPPQEEVAAQLIAENLAPSRSIPNSTPIE"
     gene            976..2874
                     /locus_tag="PKH60_04770"
     CDS             976..2874
                     /locus_tag="PKH60_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ17384.1"
                     /translation="MPNLSSLLPFGKKKQVENQAPTKEQQELEKIKNFSRGLVTIQDI
                     IAPEAIEIDFTFQKINSTYTRTLFVAGYPRSVPANWLSPLINYPHRISTSMFIYPVDS
                     SEILDNLKRKITEMEAEIQSDLRAGKISNINTEVKLEDARYLREDLAKGSERFFQFGL
                     YVTITAVSKEELDRVTKAIQSSLGSLLIVSKKATLQMDEGFKTTLPMGTDKLLVTRNM
                     DTTSLATTFPFTSSELTQENGIMYGINEHNESLVIFDRFSMENANMLIFAKSGAGKSF
                     TVKLEVLRQLMFGTEAIILDPEHEYEEMCRAVDGEYINFTFSSQAKINPFDLSALYEE
                     GENELGQKIISLHGLLKVMLGDMTPQQDALLDRALVAAYKAKGITPDPSTQTNEPPLM
                     EDLYKALIGMENKEADDISARLEKFITGSFRGIFDQGSNVDITNPLTVFSVKEMEEGL
                     RPIAMYIILDFIWTRVKKNLKKRILIIDEAWYFMKHPDSASFIHSMVKRARKYFLGIT
                     TITQDVEDFLHNDFGKAIVSNASIQFLMKQSTASIPVLAETFYLSQGERQLLVTADVG
                     EGIFFAGQNHVALRVVASPEEHSLITTDPEELLQRQEQRATAVVNSSAFTGSTIADYS
                     PPNQNTQG"
BASE COUNT          969 a          670 c          600 g          826 t
ORIGIN      
        1 tcttgatatt aaaaaacggc acatccgcca tcatcctaac tgttgacgcc atgaactttg
       61 gcttacttgc agaggaagag caggatgcca ttatgtatgc gtatgctggt ttgttaaatt
      121 cacttaatta tcctattcaa attgttgtgc aatcaaaaac gaaagatgtg actggctatc
      181 tcagaaaact aaccgagcaa gaagaaaaaa catcaaatga aaccatgaga aaaagaattg
      241 ctcagtatcg tttgtttgta gccaatctaa ttcacgaacg aaatgttttg gacaagcgat
      301 tttatgtggt aattcctgcc acctcaattg aaatggggtt tctaggggca caaacttttg
      361 tccccggtaa agaagatttc aatatcagcg acgttgaaag aagcgtagtg attgaaaaag
      421 ccaccaactt gcttgaaccc aagcgagacc acctcatcag ccagtttgct cggattggtt
      481 tatattcaag acagctcaat acccaagaaa ttattcagtt gttttatgtt aattacaacc
      541 cagagtcaac tgagggacaa gaaattgctg aaagttctgc ctacaccagt gggttggtgc
      601 acgctgatat gggaacagca tatggagatg ttgctgtcag cccagtaacc aatgcatacg
      661 aaagtaatac agatccacaa ccacaaagtg cgcctgtctc agaggctcaa ccagccccat
      721 ccccaagcca atcaccaagt gccctcaatc aggttaatca agaacaaata gtttggtcgc
      781 aacctcagca gtctattcag gaaccaactc aagcaaatgt gcaggaccca tttcattcac
      841 ctgttcaaac ccaagctcaa agtggctacc aacctccaag cagcaatcca cctcaagagg
      901 aggttgcagc ccaactcata gctgaaaatc tagccccatc tagaagtatc cccaacagca
      961 cccctattga ataatatgcc aaacctatca tccctactcc catttggaaa aaagaaacaa
     1021 gttgaaaacc aagctcccac caaagaacaa caagagttgg aaaaaataaa aaactttagt
     1081 cgaggtttgg taactatcca agacataata gctccagaag ctattgagat tgactttact
     1141 tttcaaaaaa ttaactccac ctatactcga acactgtttg tggctggata tccccgaagc
     1201 gtacctgcca actggctctc tcctctcatc aactatcccc acagaattag cacctcaatg
     1261 tttatttatc ccgtagattc ctctgaaatt ttagataatt tgaagcgaaa aattacagaa
     1321 atggaagcag aaattcagtc agacttgcgg gcgggaaaaa tcagcaatat taacaccgaa
     1381 gttaagctgg aagacgcacg ttacttgagg gaagatttgg ccaagggctc agagaggttt
     1441 tttcaatttg gtttgtacgt taccattacc gctgtttcta aagaagagct tgatcgagta
     1501 accaaggcaa ttcagtcatc acttggatcc ttgttaattg tgtcaaaaaa agccaccctc
     1561 caaatggatg aaggttttaa aaccactcta cccatgggaa cagacaagtt gttggtaact
     1621 agaaacatgg atactacttc gctcgccact accttcccat ttacctcaag tgagctgact
     1681 caagaaaacg gcataatgta tggcattaat gaacacaatg agtcgttggt tatttttgat
     1741 cggttttcaa tggaaaatgc caatatgttg atttttgcga agtctggagc tggtaaatca
     1801 tttacggtaa aacttgaggt tctgcgtcaa cttatgtttg gcaccgaagc cattattctt
     1861 gacccagaac atgaatatga agaaatgtgt cgagcggttg atggtgaata tattaacttt
     1921 acctttagtt cgcaggccaa aattaaccct tttgatctct cagctcttta cgaggaggga
     1981 gaaaacgaat tgggacaaaa aattatttcc cttcatggat tactcaaggt tatgttgggc
     2041 gacatgaccc cccaacaaga cgctcttctg gacagagctt tggtagccgc ctataaagct
     2101 aagggcatta ctcccgaccc atccacccaa acaaatgagc cgccccttat ggaagactta
     2161 tataaagccc taataggtat ggaaaacaaa gaagctgacg atatcagtgc tcgtttggaa
     2221 aaatttatca ccggctcatt taggggaatt tttgatcaag gttcaaatgt tgatattacc
     2281 aaccctctta ccgttttctc agttaaagag atggaggagg gattgcgccc aattgctatg
     2341 tatatcattc ttgattttat ctggacgaga gtaaaaaaga atctcaaaaa gcggatttta
     2401 atcattgatg aggcttggta tttcatgaaa caccccgatt ctgcttcatt tattcactca
     2461 atggtaaaaa gagctcgcaa atacttcctt ggcattacca ccattactca agatgttgaa
     2521 gactttttac acaacgactt tggtaaagcc attgtgtcca acgcctccat ccagttttta
     2581 atgaaacaat caactgcttc aattccagtt ttagcagaaa ctttttacct ttcccagggt
     2641 gaaagacaac tgctcgtcac cgcagacgtt ggagagggta tattttttgc tggtcaaaac
     2701 cacgttgcgc tgagagtggt ggctagtcca gaagaacact ctttaattac cacagatccc
     2761 gaagaactac tccaaagaca ggaacagagg gcgactgcgg ttgtaaattc gtctgctttt
     2821 actggatcaa ctattgctga ttattcccca cccaatcaaa atactcaagg ataaagctac
     2881 tcatcggtgt ttttttccac ttgcagaatg tgggaaactt tgtgtataac tgccaaatag
     2941 atttggggtg gaaatatgtc tctacaagcg ttatcaaaag acgaagtctt attggtttgg
     3001 aacaaaacct gctcaaagct ggagagccaa ctttctccag cggtatttaa tacgtggatt
     3061 ttagc
//
LOCUS       DAOQZP010000080         2956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_35789_length_2956_cov_4.551534, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000080 DAOQZP010000000
VERSION     DAOQZP010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35789_length_2956_cov_4.551534"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PKH60_04775"
     CDS             complement(<1..285)
                     /locus_tag="PKH60_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17385.1"
                     /translation="MKKTTKINIQQHLTDIRVREKQNSEFLHLTINENQMSKLANSFL
                     NSKLSERYYFGGGKDGIVDFVSFTFVGMPEVENIVNDVKQALMEMTGATVA"
     gene            428..1102
                     /locus_tag="PKH60_04780"
     CDS             428..1102
                     /locus_tag="PKH60_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNQ17386.1"
                     /translation="MKLLVVEDEHRIATYIKKGLEMKSHVVDLAYDGESGYDLASSEK
                     YDVIILDRMLPKLDGVTVILKLREENNHVPILLLTAKTEVEDRVEGLEAGADDYLGKP
                     FAFTELLARVRALGRRPKARESSIITVADLTINTSNFQVSRAKQPINLSKKEFTLLEF
                     LARHHGQVLTIDQITQQVWEYDSDVLPNTAQVYIGYLRKKIDQPFISAPLIKTVRGFG
                     YQFGEK"
     gene            1099..2118
                     /locus_tag="PKH60_04785"
     CDS             1099..2118
                     /locus_tag="PKH60_04785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNQ17387.1"
                     /translation="MNSLFTLARIKLTFWYVSIVVVLTASISTLFYLRTSQVISTEYE
                     RINRRIEREWIGMSPPPGAGMGRHITIEDLSEAKQQIIWQLLGINVFVVTFFSIVSYW
                     LTGKTLKPIQQSHEAQKQFVGDAAHELKTPITALKTSLEVNLLDRTLSKETKQILKEN
                     LTDVIQLESLSHQLLNLARIEDRAIELQEIPFQPILSEVLKTVSGLAKKKQISLVVKQ
                     PKQLLRVKVNKGMLKEAILTLLDNAIKYSPSKSQVTIELTSSQSMVHLSVHDQGSGIA
                     KTDLPHIFKRFYRVDQSRNKQVANGYGLGLSIAQSIITQHHGKITVNSTVGKGSTFTI
                     QLPLI"
     gene            2206..2718
                     /locus_tag="PKH60_04790"
     CDS             2206..2718
                     /locus_tag="PKH60_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17388.1"
                     /translation="MKAKLLLPIAAVAITAITMYGANQIMAFSGGNQHDALIQKLAST
                     FGKSETEVQTVFNQYRTEQQATREAEFEARLATAVTEGKLTEEQKQLVIAKHAELQAQ
                     HEADFATKDSMTREEWQAKRQAEHDVLDSWAESNGIDLSYVMPERGPMGGGKGRGMHG
                     EGFGQGFGQE"
BASE COUNT          942 a          566 c          591 g          857 t
ORIGIN      
        1 agccactgta gcccctgtca tttccattag tgcttgtttc acatcattaa caatgttttc
       61 gacttctggc ataccaacga atgtaaatga aacaaaatca actattccgt ctttacctcc
      121 tccaaagtaa tagcgttcag aaagtttgct gttgagaaaa gagtttgcta gttttgacat
      181 ctggttttca tttattgtca gatgtaaaaa ttcggaattt tgtttctctc ttactctgat
      241 atctgtcaag tgttgttgaa tatttatctt ggtggttttt ttcataactt tacacagaag
      301 tagttaatat tcataagtat acatattggt tgtgtattat atcagcatat caattatttg
      361 tcaatagtta ttgttataag tcttttatct caaccaattc tcaagttagg aggtataata
      421 gagtagtatg aaactattgg tagttgaaga cgagcatcgg attgcgacgt atatcaaaaa
      481 agggttagag atgaagtcgc atgtggttga tctggcttac gatggagaat cagggtatga
      541 cttggcatct tcagagaagt atgatgtgat aattttggac aggatgttgc caaaactgga
      601 tggtgttacg gttatcttaa agttgcgtga agaaaataat catgtgccga tattgttatt
      661 aacggcgaaa acagaggttg aagacagagt tgagggtttg gaggcggggg cagatgatta
      721 tttgggtaaa ccgtttgcgt ttactgagct attggcaagg gtgcgggctt tgggccggcg
      781 gcccaaagcc cgcgaatctt caataatcac cgttgctgac ctgaccatta atacatctaa
      841 ttttcaggtc agccgtgcca aacaacctat caacttatcc aaaaaagaat ttactttact
      901 tgagttttta gctcgtcacc atgggcaagt tttgactatc gatcaaatta cccaacaagt
      961 ttgggagtat gatagtgatg tactacccaa tactgcgcag gtgtacattg gctatctcag
     1021 aaaaaaaatt gaccagcctt ttatatcagc accattaatc aaaactgttc gtgggtttgg
     1081 ctatcaattt ggagaaaaat gaattcattg tttacattag ctagaatcaa acttactttc
     1141 tggtatgtca gtattgtagt agtactcaca gccagcatta gtacattatt ttatttgaga
     1201 acttcacaag ttatttccac agaatatgaa cggattaatc gtcgaataga acgagagtgg
     1261 attggtatgt ctcccccacc tggagctggc atggggaggc acataacaat tgaagatttg
     1321 tccgaagcca agcaacaaat tatttggcag ttactcggta ttaatgtatt tgtcgtgact
     1381 ttcttttcaa tcgtcagcta ttggctaacc ggaaaaacgc tcaagcccat tcaacaaagt
     1441 catgaggctc aaaaacaatt tgtaggtgat gctgctcatg aattgaaaac tccaatcacg
     1501 gcattaaaaa cttcactgga agtaaatcta cttgatcgaa cactatccaa agaaacaaaa
     1561 cagattttga aagagaatct tactgacgtt attcaacttg aatctctcag tcatcaacta
     1621 cttaatctgg ctcgaattga agatcgggcg attgaactac aagagattcc atttcaacca
     1681 attctatctg aggtactaaa aaccgtttct ggattagcca aaaagaaaca gatttcatta
     1741 gtagtcaaac aaccaaaaca attgcttcgg gtaaaagtta ataaaggcat gctcaaagag
     1801 gcaattctca cgctgttaga taatgccatc aaatatagcc caagcaaatc gcaagttaca
     1861 attgaattga cctcatcaca atcgatggtc cacctgtcag tccatgatca aggatctgga
     1921 atcgctaaaa ctgacttacc tcatattttt aaacgttttt accgagtcga tcaatccaga
     1981 aataaacaag ttgcaaatgg ttacggactt ggcttatcta ttgcacagtc aatcatcact
     2041 cagcatcacg gtaaaattac tgtcaactca acagttggca agggtagcac cttcaccatt
     2101 caacttcctc ttatataaac gaaggtttac actaaagcat tctcagcaat ttcttatctt
     2161 tgttctgtat attcttttct tagtacaaat acagaaagga aaactatgaa agcaaagctt
     2221 ttactcccca tcgccgcagt ggccatcact gccatcacta tgtatggtgc caaccaaatt
     2281 atggcattta gcggtggaaa tcagcatgat gcgctgattc aaaaattagc atcaaccttt
     2341 ggtaaaagcg agactgaggt tcaaacagta ttcaatcagt acagaactga acaacaagcc
     2401 actcgcgaag ccgaattcga agctcgattg gctacagcag taactgaagg gaaactaact
     2461 gaagagcaaa aacaattagt tattgccaaa catgctgaac ttcaagcaca acacgaggcg
     2521 gactttgcta ctaaagactc tatgactaga gaagaatggc aagcgaaacg ccaagctgaa
     2581 catgatgtac ttgatagttg ggcagaatca aacggcattg atctcagcta tgtaatgcct
     2641 gaaagaggcc caatgggtgg tggcaaaggt agaggaatgc atggagaggg atttggtcaa
     2701 ggatttggtc aagaataaaa tggtcaagtc aaaataatcg ggaggaacta tagcgctctt
     2761 ggcatcagcc aagagcgcta ttttatttcc tatctaactc attaaagaca ttctgcaaca
     2821 attcaactag cttttccatt tgtccctgag aaagctcatg acttggactg actccatcag
     2881 catgttgaaa tggtcgaata gtccctagac gctctgctaa aaacaatggt ccatacagtg
     2941 tagattgcac tttgcg
//
LOCUS       DAOQZP010000081         2896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_36927_length_2896_cov_5.484688, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000081 DAOQZP010000000
VERSION     DAOQZP010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36927_length_2896_cov_5.484688"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PKH60_04795"
     CDS             complement(<1..503)
                     /locus_tag="PKH60_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17389.1"
                     /translation="MLSAITSFILALGITPLVSWFYQKKRWVDDPKIKKHAKKLHSQP
                     IPRGGGIALYLAIAAPALFFLEIDKYLIAILIGGLILTIIGFIDDIYDIHPMFRLLTN
                     LLVALVVVGSGIGIAYVSNPFGQGVIHLDQPQISFWLFQSQHTIWLLADLFAVLFIIW
                     NMNSINWS"
     gene            complement(511..870)
                     /locus_tag="PKH60_04800"
     CDS             complement(511..870)
                     /locus_tag="PKH60_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17390.1"
                     /translation="MNFFSLGALLYANQQSRIPLLISLSLTAATTAITIIFLASSQPV
                     LPILYTLTRPEQTLVAKWWLLLIPMFSLLFSLIGHVVYLTLQSIDQFILQLFTWFSVF
                     TQVVLLLALLRIIFITA"
     gene            complement(890..2554)
                     /locus_tag="PKH60_04805"
     CDS             complement(890..2554)
                     /locus_tag="PKH60_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17391.1"
                     /translation="MKKRLIDLWWKVDHSFERRLPLVLLFALVVFLRIPSYFEPYWYG
                     DESIYLTVGTALNQGKTLYRDIVDHKTPIIYYLARVPNQLSFRILLTLWMFPVVAAFF
                     FITTKVLKKKRLALIATATLIVATTLPTFEGMIPNGELFVLGFFLPGLALLYSVLHSA
                     KLQQYPSLKASSFFHLHQLIALARCTLNLKKQLLLIVAGFLFGCAVLTKVPAIFDVIA
                     AYLLVAFFILGIKRNLTSKKLNLSRVIQLLVIYFWLTLGFATPIVISVAYFTLLGAFS
                     DYLAFGLLYNFHYTSTWVPTSTLPIATLFFSLKGKLLILSGITFIVVYLRKKLPPIFL
                     FAIAWTWWSAIAASLSNRPYPHYFIQVIPSVLLLLFVFIDTIPTIFQKKLSQLKTYGI
                     QSFLFTVTIGMLIFINITLGLWSNRYSTTKYYQRFLSLLNGSVTLASYQDSFDSLVAD
                     NRQAAKLIKSENPNEIFIWGTNPSLYAQTHTSPANKFTVLFHVADLGLYQETVEAVVA
                     NSPEFFIVMKNAEDLPAPLQRYLVNNYSPAQNLEYMAVWQRRSTRY"
     gene            complement(2600..>2896)
                     /locus_tag="PKH60_04810"
     CDS             complement(2600..>2896)
                     /locus_tag="PKH60_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17392.1"
                     /translation="PPLLNPEFGRTILGKTLPQVTSTLLTLSLVSMVVIFLIDALNRP
                     PKPGGRNAFSWILQPLEFLLLPVLGFFFSALPGLDAHTRLLLGKYIEYRVTEKV"
BASE COUNT          987 a          478 c          649 g          782 t
ORIGIN      
        1 gaccaattaa tactattcat gttccaaata ataaaaagta cggcaaacag atctgcgagt
       61 agccatattg tatgttggct ttgaaataac caaaaagaaa tttgaggttg atcaagatga
      121 atgactcctt gaccaaaagg attggaaaca tacgcaatgc caattccaga gccaactaca
      181 acgagtgcta ctaataggtt ggtaagtaaa cgaaacattg gatgaatatc ataaatatcg
      241 tctataaaac caataatcgt tagaattagt ccaccgatga gaatagcaat aaggtacttg
      301 tctatctcaa ggaaaaagag agctggggct gcaattgcaa gatacaaagc aattcctcca
      361 ccgcggggga ttggttgaga gtgtagtttt tttgcatgct tttttatttt tggatcatca
      421 acccagcgtt ttttttggta gaaccaagaa acgagtggtg ttatacctag ggctaaaata
      481 aatgatgtaa tggcgcttag catgtaaaac ttatgctgta ataaaaatta ttcttaagag
      541 ggctaatagt aagactacct gtgtaaagac agaaaaccaa gtaaaaagtt gcaagataaa
      601 ttggtcaata gattgtagcg taagatatac cacgtgacca atgaggctga acaaaagcga
      661 gaacattggt ataagcaaga gccaccattt tgcaactaat gtttgttcag gtctggttag
      721 tgtgtataaa attggtaaaa cgggttggga actagcaaga aatataatcg taattgcggt
      781 cgttgccgct gttaaactta aacttattag caatggtatt cttgattgct gattggcata
      841 caataaagcg cccaagctaa agaaattcat atcagtattg tagcctatct taataccgag
      901 tggatcgacg ttgccaaacg gccatatatt ctagattttg agcaggggaa taattattga
      961 ccaaatatct ttgtaaggga gcgggtagat cttcagcatt tttcatgaca ataaaaaatt
     1021 caggagaatt tgccaccacc gcttcaacgg tttcttggta caggcccaaa tctgctacat
     1081 gaaacagtac ggtaaattta tttgctggag aggtatgtgt ttgagcatag agactggggt
     1141 ttgttcccca gataaatatt tcatttggat tttcagactt gatcaactta gctgcttggc
     1201 gattatctgc cactaaggag tcaaaactat cttggtatga tgcgagtgtg acagaaccat
     1261 ttagtaggga aagaaaccgc tgataatatt tggtagttga atagcgatta ctccataaac
     1321 caagggtgat attgatgaaa atcaacatac caatagtcac tgtgaagaga aaactttgaa
     1381 taccataggt ttttaattgt gagagttttt tctgaaatat tgtaggaata gtatcaataa
     1441 agacaaataa gagcagtagt acggagggta tcacttgaat gaaatagtgt gggtatggtc
     1501 gattggacaa tgacgcggca atcgctgacc accaagtcca agcaatggca aagaggaata
     1561 ttggtggtaa tttttttctt aaatacacca ctatgaacgt aattccactt aaaattaaca
     1621 gttttccttt cagcgagaaa aagagagtgg caatgggtag agtgcttgtg ggtacccaag
     1681 tggatgtata gtgaaagttg tagagtaaac caaaggcgag ataatcactg aatgctccga
     1741 ggagagtaaa atatgccact gagattacta ttggagtcgc aaatcccaaa gttaaccaaa
     1801 agtagattac tagaagttga attactctgg ataaattcag ttttttggat gtgaggttgc
     1861 gctttatacc gagaataaaa aaagcgacaa gtaaataggc ggcaattacg tcaaagatag
     1921 cgggtacttt tgtcaatact gcgcagccaa aaagaaatcc agcaacgata agcagaagtt
     1981 gtttttttag gttgagagtg caacgagcca atgcgattag ttggtgaaga tgaaaaaaag
     2041 aggaagcttt tagagagggg tactgttgaa gtttggcaga atgtaacact gagtacagga
     2101 gagctaatcc tggcaaaaag aatccaagta caaataattc tccgttgggt atcatgccct
     2161 caaaagttgg tagggtggtc gcgactatga gcgtggcagt tgcaatcaaa gctagccgct
     2221 tttttttcaa gacctttgta gttatgaaga aaaaagcggc aactaccgga aacatccaaa
     2281 gggtcagcag aattctaaaa ctaagctgat tgggcactcg agcgaggtag taaataattg
     2341 gcgttttatg atcaacaata tctcggtaca gagttttgcc ttgattgagt gcggttccca
     2401 cggtgagata aatagattca tcaccatacc aatatggctc aaaataggac ggtattcgga
     2461 ggaatacaac cagagcaaat aacaatacca acggcaaacg tcgttcaaag gaatgatcta
     2521 ccttccacca caaatcgatt agtctttttt tcacgctata ttcagtgtaa atgatctatg
     2581 gtgcgaatgt aaataccgat tagactttct ctgttactcg atactcgata tattttccaa
     2641 gcaataacct tgtgtgagca tccaatccag gcaacgctga aaagaaaaaa cccaagactg
     2701 gtagcagtaa aaattcaagt ggttgcaaaa tccaagaaaa agcatttctg ccccctggtt
     2761 tgggaggacg attgagtgca tcaattagaa aaattacaac catggacacg agcgagagcg
     2821 tcaaaagagt agacgtaact tgaggtaggg tttttcctaa aatggtacga ccaaattctg
     2881 gattcaacag tggggg
//
LOCUS       DAOQZP010000082         2864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_37567_length_2864_cov_3.553578, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000082 DAOQZP010000000
VERSION     DAOQZP010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37567_length_2864_cov_3.553578"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PKH60_04815"
     CDS             <1..365
                     /locus_tag="PKH60_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phBC6A51 family helix-turn-helix protein"
                     /protein_id="HNQ17393.1"
                     /translation="QKIPIVQVCCEKLSIARSTYYRWREESPEFAKQADKALAEGVAL
                     INDLTESQLLSAIKNGNISSIFYWLNHRHSVYSNKLEVTTKLDKETLTVEQEELIKKA
                     LEHASLIMKGGHNDANNQ"
     gene            346..1911
                     /locus_tag="PKH60_04820"
     CDS             346..1911
                     /locus_tag="PKH60_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17394.1"
                     /translation="MMPIINDDLLKKVITERPLRVSVTKESLLFFSSVYFPHYFGTDF
                     AQFQYDMIQIMEDKDCQFAVVITFRGSGKSTLCTTISSLWAIMGSQEKKHVLIVCGTV
                     VQAKAQMATIKDELESNKLLRSDLGPFKESNNEWNSTSLEFSKYQAKITAISIDQSMR
                     GLRYKQHRPDLVFIDDIEDSSSVKTKEGRKRIQELYSSEIAPIGDLKKTKMVLLGNYL
                     HPNSLLATLREKIRAGKIHGRELFVPIMSDDGIPAWPQMFPTEESLEQLKERLSDHRA
                     WMLEYMLKAVGDEDQLVTYDDIAWYDSIPRGWEPYFQFNAAGVDLAISKNDRADLTAI
                     VPGSVFRNNGIFHLFIDPNPTNRRLNFRETVDFLMDFKNVYPDTHLFIEGGSYQSSLT
                     EQLLHEGIDAHKVDIGKLSKLERLAAVAFWIRKGQIHFPKDRCTDLVNQMVNFGSEPH
                     DDLVDAMTLLILQVMHYAKTGEFPSSLSSEGIRIRSSVNDWRKPRSLEVYDRNWLKRL
                     GIDKDVWGTKRGR"
     gene            complement(1895..2107)
                     /locus_tag="PKH60_04825"
     CDS             complement(1895..2107)
                     /locus_tag="PKH60_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNQ17395.1"
                     /translation="MRKNAKLPKVLGKKVQKARKETGLSQETVAFKVGVSRAYMGYIE
                     QGRNVPSLEVLQKIAKVLKVKLSDLV"
     gene            2221..>2864
                     /locus_tag="PKH60_04830"
     CDS             2221..>2864
                     /locus_tag="PKH60_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17396.1"
                     /translation="MPIIVSQNGRNATKLDKISFGLENKLQAYIYDNPESIPLYDIKD
                     DIRLLILAREFGTTSGPIDAIGIDGDGQLYLVETKLYKNPDKRTVVAQVLDYGASLWK
                     SLNDFDDFLLQLNGHVQKKFGMSATEKIKTFFGIDDERLQNVLEQMKLNLDEGNFKFV
                     VLMDSMEDRLKDLIVYMNQNSKFDIYCVELEYYQHDSFEILIPKIYGAQVKKDVG"
BASE COUNT          876 a          586 c          638 g          764 t
ORIGIN      
        1 tccaaaagat accgattgtg caggtgtgtt gcgagaaatt gagcattgct cgttcaacat
       61 actatcgctg gagggaggaa agcccagaat ttgctaagca ggcagataaa gccttggctg
      121 agggagtggc actgattaat gatcttaccg aatctcagct gttgtcggcc attaaaaatg
      181 gcaacataag tagcattttt tactggttaa accatcgcca ctctgtttac agtaataaat
      241 tagaggttac cacaaaactc gacaaagaaa cattaacagt cgaacaggag gagctcatta
      301 aaaaagctct cgaacacgca tcactcatca tgaaaggagg ccacaatgat gccaataatc
      361 aatgatgatc ttctgaaaaa agtaataaca gagcgaccgc tgcgggtgtc agtaacgaag
      421 gagagcttgc tgtttttttc atcagtttac tttcctcatt actttggtac cgactttgcc
      481 cagttccaat acgacatgat tcaaatcatg gaagacaagg actgccaatt tgcagtagtc
      541 attacctttc gtggatctgg caagtcaaca ctgtgtacca caatatcatc actttgggca
      601 attatgggaa gtcaggagaa gaagcatgta ttaatagtat gtggcactgt tgttcaggct
      661 aaagcccaaa tggcaactat taaggatgag ttggaaagca ataagttgct tcgatctgat
      721 ttgggtccgt ttaaggagag taataatgaa tggaactcca cttcattaga attttctaaa
      781 taccaagcca aaatcactgc aatatctatt gatcagagca tgcgtggctt gcgatacaaa
      841 caacatcgtc ctgacttagt tttcatagat gatattgaag actcgtcatc ggttaaaacc
      901 aaagaaggtc gcaaacgcat tcaagaactc tacagcagtg aaattgcacc aataggtgac
      961 ctgaagaaaa ccaagatggt tttgctcggt aactacctac atcccaactc actcctggct
     1021 acgctgcgtg agaagattag agcgggaaaa attcatggtc gggagttgtt cgtaccgatt
     1081 atgagcgatg atggcatacc agcgtggcct caaatgtttc ccacggaaga aagtctagag
     1141 caactcaaag agcgattgag tgaccatcgt gcttggatgt tggagtacat gctcaaagcc
     1201 gttggcgatg aagaccagct agtcacctat gacgacatag cttggtatga ctcaatacct
     1261 agaggatggg aaccgtactt ccaatttaat gctgctggag ttgacctagc gattagcaag
     1321 aatgaccgag ctgatctaac cgccatcgtg ccagggagcg tatttaggaa taatggtatt
     1381 tttcacctgt tcattgatcc aaacccaacc aatcggaggc ttaacttccg agaaacggtc
     1441 gactttttga tggattttaa gaacgtctat ccagacaccc acctctttat agaaggtggc
     1501 agttaccagt catctttaac cgagcaacta ctccacgaag gaattgatgc tcacaaggta
     1561 gatatcggca aattaagcaa gctagagcgg ttagcggcag tggcattttg gatacgtaaa
     1621 ggccaaatcc attttcccaa ggataggtgt acagaccttg taaaccagat ggtgaacttt
     1681 ggctcagagc cacacgatga cttggtggat gcgatgacac tcttaatttt gcaggtgatg
     1741 cactacgcca aaaccggcga gttcccttca tcgttaagta gtgagggtat ccgcataaga
     1801 tcatcagtaa atgattggcg aaaaccacgc agtttagagg tgtacgacag aaactggctc
     1861 aaaagactag gaattgataa ggatgtttgg ggtactaaac gaggtcgcta agcttcacct
     1921 tcaacacttt ggcgattttt tggagcactt ctagcgaggg gacgttacgt ccttgctcta
     1981 tgtaacccat ataggcacga ctgacaccaa ccttaaaagc aactgtttct tgtgacaaac
     2041 cagtttcttt acgagccttc tggacttttt tgccaagcac tttgggcaat ttggcatttt
     2101 ttctcatgcc ctaattgttt agtaaaaagg cttttcactc cacttagtga gagttagcaa
     2161 tttcctctgg tattgagctt gcttttatgc taactctggc ttatcattta gtcaggaaat
     2221 atgccaataa ttgtgtccca aaacgggaga aacgccacta aattagacaa aatcagcttc
     2281 ggtttggaaa ataaactgca agcctacatc tacgacaatc cagaaagtat tcctttatac
     2341 gacatcaaag atgatattcg cttactgatt ttggctcggg agtttggaac aaccagtgga
     2401 cccatcgatg ctataggtat tgatggcgat ggtcagctct atttggtaga aaccaagctc
     2461 tataaaaatc ccgacaaacg aacggtggtt gctcaggttc tggattacgg agcttcacta
     2521 tggaagagtc tcaatgattt tgatgacttc cttctccagc tcaacggtca tgtacaaaaa
     2581 aagtttggca tgtcagcgac cgaaaagatc aaaaccttct ttggtattga cgatgagcga
     2641 ttacaaaatg ttctagagca aatgaagctt aacctagacg aaggcaactt taagtttgta
     2701 gtgctaatgg acagcatgga agacagactc aaagatttaa tcgtctacat gaaccaaaac
     2761 agcaagttcg atatttactg tgtggagttg gagtactacc aacatgatag ctttgaaatt
     2821 ctgatcccta agatctatgg tgctcaagtc aaaaaagatg tggg
//
LOCUS       DAOQZP010000083         2829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_38265_length_2829_cov_4.956381, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000083 DAOQZP010000000
VERSION     DAOQZP010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38265_length_2829_cov_4.956381"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PKH60_04835"
     CDS             <1..534
                     /locus_tag="PKH60_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17397.1"
                     /translation="CKTKSKSFVFIRTDGDSEKVILRTSNYGFEELWTYKRACEVLPD
                     FVTCHFYPELADQPTSGVKPKINPVTKGIGFREVFAGASLGEYRHYELPEPIKQSIDQ
                     QVKYIRLMLIIHGIVHNHDHLNNFNVRFLLSSPDGGEKSISFDLNAAIQVAVERGWNL
                     APIVTLRDWDMATITAK"
     gene            complement(543..1226)
                     /locus_tag="PKH60_04840"
     CDS             complement(543..1226)
                     /locus_tag="PKH60_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNQ17398.1"
                     /translation="MSIFKLLKQGIILLETAFFSRYSDPEKYQSYVVESGRQALAEEV
                     ASVVLVASGKSTTAKLKVLDVAAGTGLVSKALLNVSCSVVAFDHSAEMLGSLSKQYKG
                     IITKVGDMNQPWPFANEQFDAVTIVWGNRYIVDPEHFSQEVFRVLKPGGTLVWPIFWL
                     EQPLWWLFLLRNRGLSQLFSIFQLTPTGLAKMLAPQKCTSIKIRSSKLGKKVGWWQKP
                     SYLIAKKAV"
     gene            complement(1230..1727)
                     /locus_tag="PKH60_04845"
     CDS             complement(1230..1727)
                     /locus_tag="PKH60_04845"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNQ17399.1"
                     /translation="MQKISTTDLVNRLLFIIGVIFVILTIFIALDPLQFIQFGYFGVF
                     VFNLFGPGTLLIPLIADHFNVVMLALVSAIGMSLNDSVVYVIGRSGQSVVKPGKRSDN
                     VRDWVVKYGRLGLFSFSLLPLPIDIVGGTVGYLSLPYSYYIVPTFAGKFLRFLALGLV
                     VIWYF"
     gene            1757..>2829
                     /locus_tag="PKH60_04850"
     CDS             1757..>2829
                     /locus_tag="PKH60_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNQ17400.1"
                     /translation="MSPHSLQKNQQLIEYQPRFASPIELLEYWTNQSPNKPALVFDSY
                     SNANPLEWSYTKLLDLVRQTQAYLLSESNGNSIAFCFSNTPEVILLNFACWSCDKVSV
                     PLDSTRDTTERKIYKIQQAKSSVLFTRSDQDTKKENAVIQSAIPELTIVEVTGVDEFL
                     QLVHFDELSVTAQNLSKETPALILYTSGTTALPKGAVLTWKSLLANAESIADWLQFTA
                     EDRWLVVMPLHHINSTTFATTTLLRGGTIVLVPAYSKSKFWPTLAQHQVTGTSIVPTI
                     AFDQLSEITTFTKHADQLTQMTRIQIGSAPVQPTTVKQFVDTFKIKLCQGYGQTETSL
                     RSTGVPMDLTEAQYQEIVASNSVG"
BASE COUNT          876 a          652 c          559 g          742 t
ORIGIN      
        1 tgtaaaacca aatcaaaatc atttgtgttt attcgcacag atggagattc tgaaaaagta
       61 atcctgagaa caagtaatta tggttttgaa gaactttgga cttacaagcg tgcttgtgag
      121 gtactacctg actttgtaac ctgccacttt tatccagagc ttgctgacca gccaactagt
      181 ggtgtaaagc caaaaatcaa tccggttaca aagggaattg gtttccgaga agtctttgct
      241 ggcgcttcct tgggagaata tcggcattac gaacttccag agccaatcaa acaaagtatc
      301 gaccaacaag taaagtatat tagactaatg ctcattatcc acggcattgt acataatcat
      361 gatcatctga ataacttcaa tgttcgtttt ttactttcct ctccagatgg tggtgaaaaa
      421 agcatttctt ttgatctcaa tgcggcaatc caggttgcgg tggaacgggg ttggaatttg
      481 gcaccgattg tcacgctgag agattgggat atggcgacga ttacagctaa ataaatactg
      541 ccttacacag cttttttcgc aattaaatag cttggttttt gccaccagcc aacctttttg
      601 ccaagtttag atgatcgaat cttaatcgag gtacatttct gaggtgccag cattttcgct
      661 aaaccagttg gagtaagctg aaaaatcgag aataactgac tcaagcctct gtttctcagc
      721 aaaaacaacc accacaacgg ttgttccaac caaaaaattg gccagaccaa tgttccccct
      781 ggtttaagaa cacgaaaaac ttcttgtgag aaatgctctg gatcaacaat gtatcgattg
      841 ccccacacaa tcgttacggc gtcaaattgc tcgttggcaa atggccatgg ctgattcata
      901 tcccctactt tggttatgat tcctttatat tgtttgctga gcgatccaag catctctgcc
      961 gaatggtcaa acgccacaac agagcaactg acattcaata atgcttttga aaccagacca
     1021 gtgccagcgg caacatcaag cactttgagc ttcgctgtgg tcgacttgcc actagccact
     1081 aaaaccactg atgctacctc ttcggccaat gcttgcctgc cggattcaac cacataactt
     1141 tgatactttt ccggatctga ataccgacta aagaaagcag tctccagtaa gatgattcct
     1201 tgctttagta gtttaaaaat tgacatgatt taaaaatacc aaataactac taaaccaagg
     1261 gctaaaaatc tcaaaaattt cccagcaaac gtcggtacga tatagtagct atatggtaac
     1321 gacaaatacc ccacggtgcc accaacaata tcaatgggca atggaagtag cgagaatgaa
     1381 aacaaaccta atctgccgta cttaaccacc caatcgcgta cattatccga ccgtttgcca
     1441 ggttttacca ctgactgacc gcttctgcca attacataga caactgagtc gttcagcgac
     1501 atcccaatcg cagacactaa agccagcatc accacattaa agtggtctgc aataagagga
     1561 attaaaagtg tccctggtcc aaacaggttg aacacaaaca cgccaaagta accaaactga
     1621 ataaattgga gtgggtcaag tgcgatgaaa attgtgagta tcacaaaaat aacgccaatg
     1681 ataaaaagca ggcggttaac caagtctgtc gtactaattt tttgcatatc caacgaatga
     1741 ccattatact agcccagtgt cgccacatag tttgcagaaa aatcagcaac tgattgaata
     1801 tcaaccgaga tttgcgtctc ccattgaatt attggaatac tggacaaacc aaagtccaaa
     1861 taaaccggcg ttggtgtttg attcatacag caatgcaaac ccattagagt ggagttatac
     1921 caagctcttg gatttagtgc ggcaaaccca agcgtatcta ctgagtgaat ctaatggcaa
     1981 ctcaattgca ttttgtttta gtaatactcc agaggtaatt ctgttaaatt ttgcgtgttg
     2041 gagttgtgac aaagtgagcg tgcccctcga ctctactcgt gacaccactg agcgcaagat
     2101 ctataaaatt cagcaggcaa aatccagcgt actcttcact cgttctgacc aggacacgaa
     2161 aaaggaaaat gcagttattc aaagtgcaat tccagagctt accattgttg aagtgacggg
     2221 tgttgatgag tttctacagt tagtccactt cgatgaactc agtgtgacag cacaaaacct
     2281 gtcgaaggag acacccgcac tgatacttta tacatctggc accactgccc tacccaaggg
     2341 tgctgtgctc acttggaaaa gcctgttagc aaatgctgaa tccattgctg attggctgca
     2401 gtttactgcc gaagatcgct ggttggtggt aatgccactg catcatatta actcaacaac
     2461 atttgctact actactctac tgagaggtgg cacaattgtg ctggttcctg cttattcaaa
     2521 atccaaattc tggccaacgc tagctcaaca tcaagttacg ggaacctcaa ttgtgcccac
     2581 aatcgccttt gaccaactca gcgaaattac cacatttact aagcacgctg atcaattgac
     2641 acaaatgaca agaatccaaa tcggttcagc acccgtccaa ccaaccacgg tcaaacagtt
     2701 cgtcgacact tttaagatta agctttgtca gggctatggc caaactgaga catcactgcg
     2761 cagcactgga gtgcccatgg atcttactga agcgcaatat caagaaatag tggcaagtaa
     2821 ttcggttgg
//
LOCUS       DAOQZP010000084         2782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_39283_length_2782_cov_5.106711, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000084 DAOQZP010000000
VERSION     DAOQZP010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39283_length_2782_cov_5.106711"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="PKH60_04855"
     CDS             complement(<1..952)
                     /locus_tag="PKH60_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNQ17401.1"
                     /translation="MPQKLRNIAIIAHVDHGKTTLVDAMFKQTSTFATHQKENQQTTI
                     LDSNELERERGVTILAKNTAVFWHDYKINILDTPGHADFSGEVERVLNMADGCILLVD
                     AAEGVLSQTRFVLQLALQLGLLPIVVINKVDRKDQRVKEVEGEIADLFLDLAHTSEQL
                     DFPTLYARGFEGIVGKQLEEQPDYSMKITDSDSLTPLFETIIEVVPEPRGIAVGGLQM
                     QINSLESDDYIGTLAIGRVFRGQIKRGQAVLVLNSDGKQRSARIEHLFVFAGLNRQPV
                     EEAQMGEIVAVAGIPKPHIGETIADVLEPEALAALSISEPT"
     gene            1062..2345
                     /gene="eno"
                     /locus_tag="PKH60_04860"
     CDS             1062..2345
                     /gene="eno"
                     /locus_tag="PKH60_04860"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007954565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNQ17402.1"
                     /translation="MQITQVDAREILDSRGNPTVEARVMVSSGEIGVAAVPSGASTGT
                     HEALELRDDDPQRYNGSGVLLAVKNVVETIGPALERMVITDQVGIDNRMLELDGTADK
                     SNLGANALLAVSMAAARAAAQAQNISLYQYIGHLFGKDSSHFELPVPMMNLLNGGKHA
                     ANSTDMQEFMVMPVGMSSFAETVRAGSEIFHKLGNLLKSQGLSTLVGDEGGYAPALKT
                     NHAPLELMVEAINQAGYRAGENVVLALDPAASEFYADNLYNLSIEQANLNSQQLVDRY
                     CQWVETYPIRSIEDGLAEDDWAGFSLLTEKIGHRIQIVGDDLFVTNPTRLERGIREKA
                     ANSILIKLNQIGTVSETIETVTIALQAGMTAIVSHRSGETEDSFIADFVVGAGTGQIK
                     TGSLNRSERIAKYNRLMQIERELGEKATITDFPFV"
     gene            2369..>2782
                     /locus_tag="PKH60_04865"
     CDS             2369..>2782
                     /locus_tag="PKH60_04865"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HNQ17403.1"
                     /translation="MTKTATRIFLTISLVLTLSLGLSGCSLSSNTQPNTTTDVSAPAE
                     VLQQAEEKENVGRPAEAEYSYVASVSGQTALDLLTQNAEVQTKEFGFGTFIEGINGVL
                     GDEKQYWAFYVNREYATEAADKTILSEGDIATFRLE"
BASE COUNT          791 a          608 c          614 g          769 t
ORIGIN      
        1 ctgtgggctc gctaatggaa agtgcggcta aagcttctgg ttctaataca tcggcaatag
       61 tttcgccaat gtgtggtttt ggaattcccg ctaccgccac tatttctccc atttgagctt
      121 cctctactgg ctgacgattc aaacctgcaa atacaaataa atgctcaatt ctagctgatc
      181 gttgcttacc atcactgttt aacactagca ctgcttgtcc acgtttaatc tggccacgaa
      241 atactcgacc aatcgccaac gtgccaatat agtcatctga ttcgagtgaa ttaatttgca
      301 tctgcaatcc acccactgcg ataccacgtg gttctggtac tacttcgata attgtttcaa
      361 atagtggagt gagtgaatca ctatcggtaa ttttcatgga ataatcgggc tgttcttcca
      421 gctgtttgcc aacaatgccc tcaaatccac gggcatataa ggttggaaag tctagttgtt
      481 cagaggtgtg agccaagtct aagaataaat cagcaatttc gccttctacc tctttgacgc
      541 gctgatcttt tctgtctacc ttgtttatca ccacaattgg caataatcca agttgtaatg
      601 ccagttggag cacaaagcgg gtttgagata aaacgccttc ggcagcatcc acaagcagaa
      661 tacaaccgtc tgccatgttt aaaacccgct ccacttcacc agaaaaatca gcgtgaccgg
      721 gcgtatccaa aatattaatt ttgtagtcat gccaaaaaac ggcagtattt ttggctaaaa
      781 tcgtgacacc acgctctcgt tctagttcat tactatccaa aatcgtcgtt tgttggtttt
      841 ctttttgatg cgtcgcaaac gtactcgttt gcttaaacat cgcatccacc aaggtagttt
      901 taccatggtc aacatgagca ataatagcga tgttacgtaa tttttgtggc ataggcgaac
      961 cttcagtaga gcaaagggtg tattgtactc caaaaagctc atcttgtgta cacaaatgat
     1021 ctttctctat actgtaggta attagcaagc gaaaggtgtg tatgcaaatt actcaagttg
     1081 atgccagaga aatacttgat tctagaggta atccaacagt ggaggctcga gttatggtca
     1141 gttctggtga aattggcgtg gcagctgtcc catctggagc atctactggc acacatgaag
     1201 cacttgagct cagagacgat gatccgcaac ggtataacgg atcgggagta ctactggctg
     1261 tcaaaaacgt ggtagaaacc attggtccag ctttggaacg aatggttata actgatcaag
     1321 ttgggattga taaccgcatg ctggagttag atggaactgc agataaaagt aatttgggag
     1381 ccaatgcgct gctagctgta tcgatggcgg ccgccagagc ggccgcccaa gcacaaaata
     1441 tttcgctcta ccaatacatt ggtcacttat tcggcaagga ctcctctcat tttgaactcc
     1501 ccgttcccat gatgaatttg ctgaatggtg gaaaacatgc ggcaaattca acagatatgc
     1561 aggaatttat ggttatgccg gttggcatgt cctcatttgc cgaaaccgtt cgggctgggt
     1621 ctgagatttt tcacaaactt ggcaatctat taaaatctca aggactctca actttagtgg
     1681 gtgatgaggg tgggtatgct cccgcactca agaccaatca cgctccgctt gagttgatgg
     1741 ttgaggcaat taatcaagct ggctaccgag ccggagaaaa cgtggtacta gcactggatc
     1801 ctgctgccag cgaattttat gctgacaatc tctacaattt aagtattgaa caagccaatt
     1861 tgaattctca acaacttgtt gatcgctatt gccagtgggt tgaaacctat ccaattcgat
     1921 cgattgaaga cggattagcg gaagacgact gggctggatt ttcattacta acagagaaaa
     1981 tcggccaccg aattcaaatt gtgggtgatg atctatttgt gaccaacccc acacgcttgg
     2041 agcgcggaat cagagagaaa gcagccaatt ctatcttaat taagctcaat caaattggaa
     2101 cggtgtcaga aaccatcgag acggtaacca tcgctctcca agctggaatg acggcaattg
     2161 tttctcaccg ctctggtgag accgaagata gttttattgc agactttgtg gtgggagctg
     2221 gcactggtca aatcaagact ggctcactta accgttcaga aagaattgcc aaatacaatc
     2281 gcctaatgca aatagaaaga gaattgggtg aaaaagccac aattaccgac ttcccttttg
     2341 tgtaattttt ggtatgctga agcgaattat gacaaaaacc gctacacgca ttttcttgac
     2401 cattagctta gtattaactc tttccctcgg cctgagcggt tgtagtttat cttcaaacac
     2461 ccaacccaat acaacaaccg atgtatctgc tccagcagag gtattacagc aagctgaaga
     2521 aaaagaaaat gttggtcgtc ccgcagaggc tgaatatagc tatgttgcca gcgttagtgg
     2581 tcaaactgct ctcgatttat taacacaaaa tgcagaagtt caaacaaagg aatttgggtt
     2641 tggcactttc attgaaggca ttaatggtgt cttgggagat gaaaaacaat actgggcatt
     2701 ttacgtcaat cgtgaatatg ccacggaagc agcagacaag acaattcttt ccgagggtga
     2761 tattgccacg tttcgtcttg aa
//
LOCUS       DAOQZP010000085         2725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_40564_length_2725_cov_4.561798, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000085 DAOQZP010000000
VERSION     DAOQZP010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40564_length_2725_cov_4.561798"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="PKH60_04870"
     CDS             complement(<1..986)
                     /locus_tag="PKH60_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17404.1"
                     /translation="MDEASPVKLVDETSNSIPVPNVPPAPPPAPPLVPSPLVNQVSPT
                     TISPATPPQYYTSQNTTVTTGSESHALRNIFLSALALGFILVLTSVSYVYWTFIRPRW
                     YLATALSNLVAAESFRFRADIPLINQSTLTFEGDRFYDNSVLSASKITYTAQGDLNQT
                     VTTTVDTIASESDEYLKPTNSHMTQMYNQLLSFEPYLQDDPALAAIQPVINGTSWAHY
                     PQSSSASTTEESEYSIAPFEFFVLLAFGTKITNYEPSMQINDQDYAHYTMSLENAAIV
                     SFLEALKDREFSIDVAELNRLIDAINQTASSNGDVLTIVVDKKLHTPHLITLT"
     gene            complement(1031..1273)
                     /locus_tag="PKH60_04875"
     CDS             complement(1031..1273)
                     /locus_tag="PKH60_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17405.1"
                     /translation="MTIKRFTTVGSVLLLCSFLMTACSVVNNNSVDQNLPASDLTTEQ
                     QEEARLLQPQSDSTELDDLDKELTDFKVLEEDFSDL"
     gene            complement(1276..1896)
                     /locus_tag="PKH60_04880"
     CDS             complement(1276..1896)
                     /locus_tag="PKH60_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17406.1"
                     /translation="MNKLPNIIIVSLAVAALYTTTYSPAFALETEDAPQMRVQNTVRT
                     TEIEQLRQEKQEAQATVTQERANQFEERREERVENRQERRADFAQQHAERLERRFSLY
                     AEHLLNVANRIETRAQLMSKNGKNTTEALAKVTEARSYITSAQSKGAEAVATFRSIDA
                     TNYDSQRAIALEARDLAEASREDFKTARDLLHEAVGILVQTQNQDE"
     gene            2034..2498
                     /locus_tag="PKH60_04885"
     CDS             2034..2498
                     /locus_tag="PKH60_04885"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNQ17407.1"
                     /translation="MVRHYSKQQVLDFLRSVSVMNLAVSSDDKPISSVLLFHVDDDFT
                     FYFGTHNDSYKAEAIRKDPNVSLSVWEHKKMLVQADGVAEEILEPVTVDLVLNDIVTA
                     TNNIKDFWPPVLRIRGNGYAVFSIKTTWMRALDLQSDAISEKELPFTMIVGD"
     gene            2501..>2725
                     /locus_tag="PKH60_04890"
     CDS             2501..>2725
                     /locus_tag="PKH60_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ17408.1"
                     /translation="MSATKKILLVEDDIFLVRVYENMMPKDRFEVDYVTDGAEVSAKV
                     AQAQYDLLLLDLVMPKKDGFEVCREIRKVSN"
BASE COUNT          758 a          469 c          642 g          856 t
ORIGIN      
        1 gtcagcgtta tcaggtgtgg tgtgtgtaat ttcttatcta ccacaatggt gagcacatct
       61 ccatttgatg atgccgtttg gttgatggca tcaattaacc gattgagctc tgccacgtca
      121 atggagaatt cgcgatcctt caatgcttca agaaaagaaa caatggcggc gttttctaga
      181 ctcatggtgt agtgagcata atcctggtca tttatttgca tggatggctc atagttagta
      241 atttttgtgc caaaagctaa aagaacaaaa aattcaaaag gggcgatgga gtattctgat
      301 tcttctgttg tcgatgctga acttgactgg ggataatgcg cccagctggt accattaatt
      361 actggctgta tggctgccaa cgcagggtcg tcttgtaaat aaggttcgaa ggaaagtaat
      421 tgattgtaca tttgagtcat gtgagagttt gttggtttaa gatactcatc actttcactc
      481 gcaatagtat ccacggtggt agtcactgtc tgattcaggt caccttgggc tgtgtacgta
      541 attttgctgg cactcagtac tgagttgtca tagaacctat ctccctcaaa agttagtgta
      601 gattgattaa tgagtggaat atctgctcga aagcgaaatg actcagcagc aactagattt
      661 gataaagctg tggctagata ccagcgtggg cggatgaatg tccaatacac atagctaacg
      721 gaagttaaca ctaagatgaa acccaaagct aacgctgata aaaaaatatt gcgaagcgca
      781 tgcgactcag aaccagtagt gacggttgtg ttttgagaag tgtaatactg aggtggagta
      841 gcagggctga ttgtcgttgg cgaaacttga ttaactagcg gagatggaac aagtggtgga
      901 gcagggggtg gtgcaggagg tacgttgggt accggaatac tgttggaggt ttcgtccaca
      961 agcttgacgg gggaagcttc atccatatga ctaacgatag cagtaattct tactgctagg
     1021 caagtagtag ctagagatcg ctaaaatctt cttcaagcac tttgaaatct gttagttctt
     1081 tgtcgagatc gtctaactca gtcgagtcag attgaggttg tagtaaacga gcttcttctt
     1141 gttgctcggt ggtcaaatcg gatgccggta gattttgatc tacagaatta ttattcacga
     1201 cactacaggc agtcatgaga aaactacaca gtagtagaac tgagccgacg gtggtgaaac
     1261 gtttgattgt catagttatt catcctgatt ttgagtttgt actaaaatac cgactgcctc
     1321 atggagtaaa tcgcgagcag ttttgaaatc ttctctgcta gcttcagcaa gatcacgagc
     1381 ttcaagcgca attgctcgtt gcgagtcgta gttagtcgca tctatcgagc gaaatgttgc
     1441 tactgcttca gcacctttgc tttgagccga agtgatatat gatctagctt cggtgacttt
     1501 tgctagtgct tcggtggtat ttttaccatt tttactcatc agttgagctc tggtttcaat
     1561 ccgattagct acatttaata aatgttctgc atacaatgaa aatcgacgtt caagtcgttc
     1621 cgcgtgttgt tgagcaaagt cagctcgacg ttcttgccga ttctcaactc gctcttctcg
     1681 tctttcttca aactgattgg ctctttcttg tgtgacagtt gcctgagcct cttgtttttc
     1741 ttggcgtagt tgctcaatct ctgttgttcg gactgtattt tgtactctca tttgtggagc
     1801 atcctcagtt tccagagcga atgctggaga gtaagttgtg gtgtacagcg cggctacagc
     1861 gagggagaca ataataatat ttggaagttt attcatgctt tgctcctttg ttatagaaat
     1921 tgatatacaa aagatacaat cttatttata acactttttc tgtagaaatc aaaataattg
     1981 ttaatcttga cagattggta atagtgtagt ctgatgaaac tataaaagat ttcatggtta
     2041 gacattactc aaaacaacaa gtgctcgatt ttcttcgttc ggtttctgtc atgaacttgg
     2101 cagtttcaag tgatgataag cccatatcat ctgtcttgct ttttcatgtt gatgatgact
     2161 tcacttttta ttttggaacc cacaacgata gttataaagc agaagcaata cgtaaggatc
     2221 caaatgttag cttatctgta tgggaacata agaaaatgtt ggttcaggcc gatggtgtag
     2281 ccgaggaaat tttggaacca gtgacagtgg acctagtatt gaatgatatt gtaacggcga
     2341 caaataatat taaagatttt tggccacccg ttttaagaat cagaggaaat ggttatgcgg
     2401 tttttagtat caaaactact tggatgagag ctttagattt acaaagtgat gcaatctcag
     2461 aaaaggagtt gccctttacg atgattgtgg gggattaggt atgtcagcga caaaaaaaat
     2521 tctgttagtt gaagatgata tttttttggt tcgagtttac gagaacatga tgccgaaaga
     2581 tcgttttgaa gtagattacg ttactgatgg cgcagaagtt tcagcaaaag tagctcaggc
     2641 tcagtatgac ttgcttttgc ttgatttagt tatgccaaaa aaagatggat ttgaagtgtg
     2701 tagagaaata agaaaagttt caaac
//
LOCUS       DAOQZP010000086         2710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_40935_length_2710_cov_3.927684, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000086 DAOQZP010000000
VERSION     DAOQZP010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40935_length_2710_cov_3.927684"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..76)
                     /locus_tag="PKH60_04895"
     tRNA            complement(2..76)
                     /locus_tag="PKH60_04895"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40..42),aa:Cys,seq:gca)
     gene            136..1047
                     /locus_tag="PKH60_04900"
     CDS             136..1047
                     /locus_tag="PKH60_04900"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNQ17409.1"
                     /translation="MTKTKTETSNSTAQPPLIVANTILTISFPWTQVEPEYQKALNKM
                     AKQLAMPGFRKGMVPPHIAEEQLKPEALVEAVLQRLLPKAYQEALEKEHKRPISDPEF
                     DPKVVTKGADWQIEVHLAEKPSVNATNYVKIAKEAKKAALVHLKEEAKKKEQSTDDSH
                     EKQHQDDHILQHIFQDLVKTCKPQLPELLVKQQTRRELERLVQELQSMKLTLDDFLSR
                     NNQTFEQLSGNIAARAVGQLQLEFILQAISQQAEITVTEAEIEEVIAKIGDEKVRKEK
                     QTETHYLEAIREHLSRKKLFDHLLTIA"
     gene            1159..1821
                     /locus_tag="PKH60_04905"
     CDS             1159..1821
                     /locus_tag="PKH60_04905"
                     /inference="COORDINATES: protein motif:HMM:NF039817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5660 domain-containing protein"
                     /protein_id="HNQ17410.1"
                     /translation="MQSQTNRGTTTGKNSSVASQNPFARALAEAEKLASQNGNQSNSA
                     SEPGFPGLDGLGGFNQFGGQKFDQTSLLREQQEKAKKERMRRQLHEQINPVDSTKLFD
                     ARQKQVKEEIDKLRYELKALSQEVRAFNKEIELTLMTEVGSHPGMEGKYYLNFFQQLR
                     AFIILLRQKIRSARTWASTAGQKKKKKMVGAGIVIDGAAHEQTKSVYDMMHHERSNAY
                     GG"
     gene            1811..2200
                     /locus_tag="PKH60_04910"
     CDS             1811..2200
                     /locus_tag="PKH60_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17411.1"
                     /translation="MVGSKERLGVEPQMNPHFFLAQLGFSNLDELFFAYQQTPFVAPL
                     LKLPYGTFDEITVISLDQFNEVISALDLRWSVPLFADRVSGHDLISYWLNYNFRSEIT
                     ISADRATVEKVRTNLIALNSEEKKNER"
     gene            complement(2209..2285)
                     /locus_tag="PKH60_04915"
     tRNA            complement(2209..2285)
                     /locus_tag="PKH60_04915"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2249..2251),aa:Val,seq:gac)
     gene            2516..>2710
                     /locus_tag="PKH60_04920"
     CDS             2516..>2710
                     /locus_tag="PKH60_04920"
                     /inference="COORDINATES: protein motif:HMM:NF017049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNQ17412.1"
                     /translation="MRHKPGKRYQSQTVFVVANNRIRTPQVRVIDDQGNMLGVMDTHV
                     AMNQAYAEDKDLVMVNDQQQP"
BASE COUNT          918 a          592 c          557 g          643 t
ORIGIN      
        1 gaggcgcggg cgggatttga acccgcgaat cgaaggtttt gcagacctcg ccattggacc
       61 actctggtac cgcgccaatt tgcaagttga ttataaccga aagccaaaat cggtatagcg
      121 tataatagcc aacttatgac aaaaacaaaa acagaaacat caaatagcac tgctcaacca
      181 ccattaatcg ttgcaaatac gattttaacc atctcgttcc cttggacaca agttgagcca
      241 gaataccaaa aagcactcaa taaaatggcg aaacagctcg ctatgccagg ttttcgcaaa
      301 ggtatggttc cccctcatat tgcagaggag caactcaagc cagaagcctt ggtagaagca
      361 gttttgcaac gcttactgcc aaaagcctat caagaagcac ttgaaaaaga acataagcgt
      421 cccatttccg atccagaatt tgacccaaaa gtggtgacaa agggcgctga ttggcaaatt
      481 gaagtacatc tggcggaaaa accatcagta aacgctacca attacgtcaa aatcgccaag
      541 gaggcaaaaa aggctgcgct ggtgcatctc aaggaagaag ccaagaaaaa agagcaatcc
      601 accgacgact cacatgaaaa gcaacatcaa gatgatcaca tcttgcaaca catttttcag
      661 gatttagtca aaacttgcaa accccagcta cctgaattat tggttaaaca acaaactcgc
      721 agagagttgg agcgcttggt tcaagagttg caatcaatga aactcaccct tgacgatttt
      781 ctcagtagaa acaaccaaac attcgaacaa ttatctggga acattgccgc tcgcgcagta
      841 ggccaattac agctcgagtt tattttacaa gcaatttctc aacaagctga aataacggta
      901 accgaagcag aaattgaaga ggtgattgcc aaaattggcg atgaaaaagt tagaaaagaa
      961 aaacagacag aaacacacta tctagaggca attagagaac atctttcgcg aaaaaaattg
     1021 tttgatcatt tgttgacaat tgcctaagct ctgcttgatt ctgcaatacg agtcgctata
     1081 atctgcgtta gtcgagagtg attcttgact ttaaaccata aaagcctata attaatgggt
     1141 ataaagtaaa cataaactat gcaatctcag acaaacagag gaactaccac tggaaaaaat
     1201 tcttccgtgg cttcgcaaaa tccatttgcg agagctctgg ctgaggctga aaaacttgct
     1261 tctcagaatg gcaaccaatc aaactccgca agcgaacctg gttttcctgg gctagatggc
     1321 cttggtggtt tcaaccaatt tggcggacaa aagtttgacc aaactagcct acttcgagaa
     1381 caacaagaaa aggctaaaaa agaacggatg cgccgtcaac tgcacgagca aattaatcca
     1441 gttgactcaa cgaaactgtt tgatgctcgc caaaaacaag ttaaagaaga aattgacaaa
     1501 ctcagatatg aactcaaggc actttctcaa gaggtacgcg ccttcaacaa agaaattgaa
     1561 cttacattaa tgactgaagt cggatcacac ccaggtatgg aaggcaaata ctacctcaac
     1621 tttttccaac aattgcgcgc ttttattatc ctgcttagac aaaaaattcg aagcgcgcgc
     1681 acttgggcaa gtaccgctgg ccaaaaaaag aaaaagaaaa tggttggcgc cggtattgtg
     1741 attgacggcg cggcacacga acaaaccaaa agtgtctacg acatgatgca ccacgaacgg
     1801 agcaacgcat atggtgggta gtaaagaacg attgggcgta gaaccccaaa tgaatcctca
     1861 tttttttctg gcacaacttg gattttctaa tcttgatgaa ttattttttg cataccagca
     1921 aacgccattc gtcgcaccat tattgaaatt accctatggc acttttgatg aaatcacagt
     1981 catatctttg gatcaattta atgaagttat ttctgcattg gatttaagat ggagtgttcc
     2041 actttttgcc gatcgagtgt ctggccatga tttaatttct tattggctaa actataattt
     2101 tcgaagtgaa ataacaatca gtgcagatcg agcaactgtt gagaaggtta ggactaatct
     2161 tattgcgttg aactcagaag agaaaaaaaa cgaacggtaa atatctcttg gtgaacttga
     2221 gaggactcga acctctgacc tcctcgatgt caacgaggcg ctctaaccaa ctgagctaca
     2281 agttcatttt acaattatat cttgccaaga ccgaggcttt aaccaaccca gctgctcacg
     2341 agcagctgga caagctgagc tacaagttca ttacaacaag acggggcaga ttatagcaag
     2401 catttcccct ctccacaagc gagccgagag tgatataatg tcacacttat gatggacgtg
     2461 gtccgttcat ctagtactat tagtaatgac tagttagacc taaggtttca aaacagtgag
     2521 acataagcca ggtaaacgat atcaaagcca aaccgtcttt gtcgttgcta ataatcgaat
     2581 cagaacgccc caagtgcggg tgattgatga ccagggaaat atgcttggag tcatggatac
     2641 acatgtagcc atgaatcaag cctacgccga agacaaagac ctagtcatgg tcaatgatca
     2701 gcagcaaccg
//
LOCUS       DAOQZP010000087         2662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_42052_length_2662_cov_4.081703, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000087 DAOQZP010000000
VERSION     DAOQZP010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42052_length_2662_cov_4.081703"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PKH60_04925"
     CDS             <1..338
                     /locus_tag="PKH60_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17413.1"
                     /translation="RQGDQQLLEISGDGEHFGYPAKLGARMLTLVPWLRQTSDQRVLC
                     VVSTDPNQSQSAWVKMLELKESTQYLRCIFSTDEVQIGEKYPLVQFNTNHKVMFTLPP
                     AGFALFQFT"
     gene            361..1137
                     /locus_tag="PKH60_04930"
     CDS             361..1137
                     /locus_tag="PKH60_04930"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNQ17414.1"
                     /translation="MQPNEGFGESVLRTFFKVFAGMMGLVFSGIVGIVILGGVAAVFT
                     QEELDEAQVAEYRVIEGDDDADVLLVSIPIEGVILGEPSANDVINYFSDLGVTYGYAI
                     KQQLTELAEDDLVDGVILEIHSPGGTIFGSKAITDGVDAYKAKTGKPVFAYIGSMAAS
                     GGYWAAASADKIIADHGVTLGSIGVIAGPFKYYDSVVSEDGGAFLGGVVTQKGISTRY
                     ITAGTSKDIGNPYREMTEAEVAILQQAVNNAYQKFVNYVS"
     gene            1183..1443
                     /locus_tag="PKH60_04935"
     CDS             1183..1443
                     /locus_tag="PKH60_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17415.1"
                     /translation="MIYDEEQSLQLELVDMIGNKQNAYQALADSISQDATYQVKQVTV
                     PLSFIETLLMSKHLFSQQPAVSVCPINASILAYHGDITSLCR"
     gene            1522..2100
                     /locus_tag="PKH60_04940"
     CDS             1522..2100
                     /locus_tag="PKH60_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17416.1"
                     /translation="MHIAFHRWRNRWLLAIAFVGISVGIATAYFTGSSWLTGQVLGTS
                     TLAIQVEQLSPSAFDSFIPGEPQEFTWKIRNTGNTPVHLAARFSGQWENPALDAGLFR
                     LSEVSYQVPGSPSWQPIAIESIVSDQPWFLSPSGQESELVALDEDSELFIRGNFVLAE
                     SADNSYQMTNFPFSLQVIAKQTTQDAVWPTYE"
     gene            2093..>2662
                     /locus_tag="PKH60_04945"
     CDS             2093..>2662
                     /locus_tag="PKH60_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17417.1"
                     /translation="MNKKILKLLLVMVLCGSASCRPAQAYFSSQTTYTSQLETADWKA
                     PSTHLLLNQQSVVGDDTEIDMDWFDCTSLEPIIVNPSNQVINLESTVEPVCVTKNFSF
                     ESNQVRTISLQYQLSAPDLAVLAQQPLVHFYWNGEEIAWLSKVARLSSWVSLPIPGDE
                     LGGKLSISVQPSRVQMMPTQLLVEQLTSQR"
BASE COUNT          741 a          531 c          612 g          778 t
ORIGIN      
        1 tgaggcaggg agaccagcaa ctactagaaa tttctggaga tggcgagcac tttgggtatc
       61 cagctaaact gggcgcccgc atgctcactc tagttccttg gttgcgccaa acctctgatc
      121 aacgcgttct gtgtgtagtc tcgaccgatc caaatcaatc gcaatctgcg tgggtcaaga
      181 tgctagaatt gaaagaatcc acccagtatc tacgctgtat tttttccact gatgaagtac
      241 agataggcga aaaatatcct cttgttcaat ttaatacaaa tcacaaagtg atgtttacat
      301 taccacccgc tggttttgcg ttatttcaat tcacttagta tttgagtata ctttagaatt
      361 atgcaaccca acgaaggctt tggcgaatcg gtattgagga ctttttttaa agttttcgct
      421 ggaatgatgg gattggtttt ctcaggcatt gttggaatag taattctggg tggagttgct
      481 gcggtattta ctcaagaaga actggatgaa gctcaagtag cggaatatcg agttatcgag
      541 ggtgacgacg acgcggatgt tttgcttgtg agtattccaa ttgagggagt aatcttgggc
      601 gagccgagtg ccaacgatgt gataaattat ttttcagatc taggcgtgac ctatggctat
      661 gccatcaaac aacaactgac cgaacttgcg gaggatgatt tggtggatgg ggtgattcta
      721 gagattcatt ctcctggcgg aaccattttt gggtccaagg ctatcactga tggcgtggac
      781 gcgtataaag caaaaactgg gaagccggtt tttgcctaca ttggctcaat ggcggcttct
      841 gggggctatt gggcagctgc ctcagctgat aaaataattg ccgaccacgg cgtgacactt
      901 ggtagcattg gagttatcgc tgggccattt aagtactacg attctgtagt gagtgaagat
      961 ggtggcgcct ttttgggtgg tgttgtcacc caaaaaggca tttcaaccag atacattacc
     1021 gctggtacat caaaagatat tggcaatcca tatcgagaaa tgaccgaagc cgaagtggcg
     1081 attttacagc aagctgttaa taatgcgtac caaaaatttg tcaattatgt gtcgtaacag
     1141 cgaagaattt ccacagaaaa aatcactaat gagcttgggg caatgatcta tgatgaagag
     1201 cagtcacttc aactagaatt ggttgacatg ataggtaata aacagaatgc ataccaagca
     1261 cttgcagatt ctatttctca agatgctaca tatcaggtta aacaagttac agtaccgtta
     1321 agtttcattg aaactctcct gatgtcaaaa cacctatttt ctcaacaacc agcagtttct
     1381 gtttgtccaa tcaatgcaag tatactagcc tatcatggcg atataacttc actctgtcgg
     1441 taaattaaat tcctctacgt ttggtgtaag catgctgtcg tatgtttgtc tttgtaattg
     1501 tatacatagc agaaaggcgg catgcacatt gcatttcatc gttggagaaa cagatggttg
     1561 ttagcgatag cgtttgtagg tatatcagtt ggaatagcga cagcatattt taccggaagc
     1621 agttggctca cggggcaggt cctcggtact agtacgcttg cgattcaagt ggagcagctg
     1681 agtccaagcg cctttgattc atttattcca ggcgaaccac aggaattcac ctggaaaatt
     1741 cgtaatacag gtaacactcc tgtccatttg gcagcccgat tttctggaca gtgggaaaac
     1801 ccagctcttg atgctggatt atttaggctt tcagaagttt cttatcaggt tccaggtagt
     1861 ccttcttggc agccaatagc aatagaatcg attgtgagtg atcaaccttg gtttttaagt
     1921 ccatctggcc aagaaagcga actagttgca ctggatgagg attcagagct atttattcga
     1981 gggaattttg tattagctga atctgccgat aattcatatc aaatgacaaa tttcccgttt
     2041 tctctgcagg tgattgcaaa acaaacgact caagatgcag tttggccaac gtatgaataa
     2101 aaagatacta aagctgttgt tggtaatggt actgtgcggg tcggccagct gccgacccgc
     2161 acaagcctat ttttctagtc aaacgactta tacttctcaa ctcgaaaccg ctgattggaa
     2221 agctccatcc actcatctat tgcttaacca gcaatcagta gtaggtgatg atactgaaat
     2281 agatatggat tggtttgatt gcacaagctt agagccaatc atagtaaatc catccaacca
     2341 agtaattaat cttgaatcta cagttgaacc agtttgtgtg acaaagaatt ttagtttcga
     2401 atcaaatcaa gtacgcacaa ttagtcttca atatcaatta tcagctccag acttggctgt
     2461 tcttgctcag cagccacttg tccattttta ctggaatggc gaagaaatcg cgtggctttc
     2521 aaaagttgcc cgtttatcat cttgggtttc actgccaatt cctggtgatg agctgggtgg
     2581 aaagttgtct atttcggttc aaccaagtag ggttcagatg atgcctaccc agttattggt
     2641 tgaacagctg acctcccaaa ga
//
LOCUS       DAOQZP010000088         2653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_42261_length_2653_cov_5.394534, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000088 DAOQZP010000000
VERSION     DAOQZP010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42261_length_2653_cov_5.394534"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="PKH60_04950"
     CDS             <1..959
                     /locus_tag="PKH60_04950"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNQ17418.1"
                     /translation="GLSALILASPWFLTFTSISEGARIVTERSELWRFLALWLSHIAV
                     TAVAAGLLIKQLLHKQAKNTHITLFILVIIGTAWILLLLPELFYMKDIYPGHPRANTM
                     FKLTFQSFILMNLIFAWLIGSLPVLIKKPLVQKITGALFVVLTFVLLIYPYFGYRDYY
                     NLKFHQYRGLNGLQWLKLENPSDYAGIIWLRQNISGRPVVLEAVGESYTTFARVSTFT
                     SFPTVLGWRVHEWLWRGSFDIPGQRSTEVQAIYEQPFDETAQTKLREYNVEYIFVGDK
                     EREAYPAINEQGLKQLGQIIFSQGTTYIIRVQDSVPLQSRVQ"
     gene            966..2198
                     /locus_tag="PKH60_04955"
     CDS             966..2198
                     /locus_tag="PKH60_04955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNQ17419.1"
                     /translation="MQLQIGCLSKVSKKYFATFVFLLLGFVFILRVTQLTQLNHYLFD
                     EDFFAYTAQLMKQNDSRIFEWWHGPLEQEVNQFSYRPPAIEWLHPPLSKSIQAVSIAL
                     FGNSPFGWRLPSVLAGIGIIWLSIEFTQLLFGKNYLSLLVGFLISTEQLITTQSKIAS
                     PDIFLAFFVLLTVWRFWHYHQKRTVFRLINVGISLGLAVSCKWSGAFILPALLVFEFV
                     FLSQSATLKTSIRPLFLLLQTTIILMFVAGSIYILSYWQLFHQGHSVQYFFDLHKQIV
                     TYQTSTSFDRPYASKPLEWLLGEKPIWYHYDQLETGEVSEIIAKPTLWLLFASELAFL
                     ISVGELISQHYRKKISKECWPKLLLLLSITSLFIPWLFISRPVFIYHFTPIVPLAIML
                     SISTFLNLLPKLSTNNSK"
     gene            complement(2175..>2653)
                     /locus_tag="PKH60_04960"
     CDS             complement(2175..>2653)
                     /locus_tag="PKH60_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17420.1"
                     /translation="GPDSLAGLVMQEKDYFALSARIKLRDAVRSELTLVGKVKLLGVT
                     LGIDEDGHISADECLPRWLINALGFDEIRINSDTFVPEDWAKNEVKMVAKYRDQVSKI
                     LKGSVPRTTLGRIEQFFFSNYQMPRPYNQHDDLAHIMSNWSEEQKLGERSFGIIGG"
BASE COUNT          742 a          571 c          483 g          857 t
ORIGIN      
        1 ttggattgag cgcgctgatt ttggctagcc catggttcct aacatttact tcaatatctg
       61 aaggagcaag aatagtaact gaaagatctg agttgtggag atttctagct ctctggctta
      121 gtcatattgc tgttacggct gttgcagctg gcttactcat caaacaatta ctgcataagc
      181 aggcaaaaaa tacacatatc actcttttca tacttgtaat catcggcaca gcctggattc
      241 tcctactcct accagaactt ttttatatga aagatatata tccaggtcat ccgagagcca
      301 ataccatgtt taaattaacc ttccaatcat tcattttaat gaatcttatt tttgcttggc
      361 tcattggatc tcttcccgtg ttgataaaaa aacctctcgt gcaaaaaatt acgggagcgt
      421 tgtttgttgt actcactttt gtacttctaa tctatcccta ttttgggtac agagattact
      481 ataatttgaa atttcatcaa tacaggggtt taaacggttt gcagtggcta aaattagaaa
      541 atccaagtga ttacgctgga attatctggt tacgacaaaa catcagtggt agaccggtgg
      601 tactagaagc agttggagaa agttacacca catttgctcg agtgtcgact tttactagtt
      661 ttccaaccgt gctgggatgg cgcgtacatg aatggctctg gcgaggaagc tttgatattc
      721 ccggccaacg gagcactgag gttcaagcca tctacgaaca accttttgat gaaacagcgc
      781 aaacaaaatt gcgcgaatac aatgtggaat atatttttgt tggcgataaa gagcgagaag
      841 cataccccgc tatcaacgag caagggctca aacaacttgg tcaaataata ttttctcagg
      901 gaactaccta catcattcgc gtgcaagatt ccgttccgct gcaatcaaga gtacaatagc
      961 gccacatgca gttgcaaata gggtgtttat ccaaggtttc caaaaaatat tttgcaacat
     1021 ttgtgttctt actactagga ttcgttttta tattgagggt aactcaactt actcagctca
     1081 accactacct ctttgatgaa gatttttttg cctatactgc tcaactaatg aagcaaaacg
     1141 atagtcgtat ttttgaatgg tggcatggtc cgcttgaaca agaagtaaat caattttcgt
     1201 atcgtccacc agcaatcgag tggctacatc caccgctgag taaatcaatt caagcagtga
     1261 gcattgcgct gtttggcaat tctcctttcg gttggagatt accatcagtc ttggcaggca
     1321 ttggtattat ttggttgagc attgaattca ctcagttact ttttggcaaa aattacttga
     1381 gtttattagt tggtttcctg atcagcacgg aacagttgat aacaactcaa tcaaaaattg
     1441 cctcgccaga catttttcta gctttttttg tgttactgac tgtgtggcgc ttttggcact
     1501 atcaccaaaa gcgcactgtt ttccggctta tcaacgttgg gatttcactt ggattagccg
     1561 tttcgtgcaa atggtctggt gcatttattt tgccagcgct attagtgttt gaattcgtat
     1621 ttttgagtca aagcgctacg ctgaagacta gtattcgacc gcttttttta ctcctgcaaa
     1681 ccaccattat tttaatgttt gttgcaggca gtatctacat tctcagttat tggcaattat
     1741 ttcatcaagg ccattcagtt caatattttt ttgatttaca taaacaaata gttacgtatc
     1801 aaacaagcac gagctttgat cgaccatatg cgagcaaacc attggagtgg ctgctaggag
     1861 aaaaaccaat ctggtatcac tatgatcaat tggaaactgg ggaagtatct gaaataattg
     1921 ccaaaccaac tctgtggtta ctcttcgcaa gcgagctcgc ctttcttatc tctgttgggg
     1981 aactaatcag ccagcattac cgcaaaaaaa tatcaaagga atgttggcca aaactactct
     2041 tacttctttc aatcacttca ctgttcattc cttggttgtt tatctctcgt ccggttttta
     2101 tataccactt cacgccaatc gttcctctag caattatgct gagtatctct acttttctaa
     2161 atctcttacc aaaactatcc accaataatt ccaaatgatc tttcacccaa tttctgttct
     2221 tctgaccaat tgctcataat atgagctaaa tcatcatgtt gattataagg tcgaggcatt
     2281 tggtagttgc taaagaaaaa ttgttctatt ctacccaaag ttgttctagg aacagagcct
     2341 ttcagtattt tacttacttg gtcgcgatac tttgctacca ttttcacttc attctttgcc
     2401 caatcttccg gtacaaaggt atcactatta atacgaatct catcaaatcc taaagcattt
     2461 attagccacc tggggagaca ttcgtctgca cttatatgcc catcttcatc aatgcctagc
     2521 gttactccaa gcagcttcac ttttccaacc aatgttaatt ctgaccgcac agcatcccga
     2581 agcttaattc tagcactcag tgcaaagtaa tctttttctt gcattactaa accagccagt
     2641 gaatctggac cca
//
LOCUS       DAOQZP010000089         2616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_43156_length_2616_cov_4.283483, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000089 DAOQZP010000000
VERSION     DAOQZP010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43156_length_2616_cov_4.283483"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..307)
                     /locus_tag="PKH60_04965"
     CDS             complement(50..307)
                     /locus_tag="PKH60_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17421.1"
                     /translation="MFAFVVLELQPDLTFNGTIAQTIGEKKSLLSSLLLVKKQGKFCL
                     LDVRKTDTIPVSLQASSFDRVLLSLNSAFEILNYQGAANAA"
     gene            complement(285..2066)
                     /locus_tag="PKH60_04970"
     CDS             complement(285..2066)
                     /locus_tag="PKH60_04970"
                     /inference="COORDINATES: protein motif:HMM:NF019149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNQ17422.1"
                     /translation="MQVALYARVSTPTQEKNDTIESQVTLLQAYAQEYNYQVLPEYIF
                     LDNGISGTRLDRPELERLRDCIQMGDVDAVLIMAPDRLARNYAHQWLLMEEFKKYNCQ
                     VIFVQTPFQDTPQGHLLLQVQGIIAEFEREQIKERTRRGRLAKAHKGEFIPWGCRAYG
                     LRYIPKQPGCPPRAEIEPQEAEVVQQMFAWLIEEQLTTRQITKRLNQQKIPTKTGKNS
                     VWHSATVRGILSNPLMTGHGYCHKTRATAQKDRRRKFTPKTTSTCNAREKRPVEEWIE
                     TTAPAIISQETFDKAQIQLAENRRQAARIYQPSSRRYLLRSLVRCGECGLFMMGTHQL
                     SVGGQYEYLYYCCVGKDPVTVGRPQRCPSRRVRADRLDQLVWQSLYQLLQQPQTLAHE
                     YQLSQQLQEGDLNQFQAQLARVDTQLQRLQRQLERLLDAYQQGLIELSELAKRRQQLL
                     EQQAALDKERQHLKLQQQRSLHWQKVEMDLQQFHQNLGLCLDRLSFEDRQKLVQLLIE
                     RVVVFSNGDVDVYHLLPTSSYSESEAALSSQKKIFEQSQFYCLRLEDRNYVSRYQTKS
                     QFRGLSSPLSDNAARPSRVFFVCVCGA"
     gene            complement(2057..2197)
                     /locus_tag="PKH60_04975"
     CDS             complement(2057..2197)
                     /locus_tag="PKH60_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17423.1"
                     /translation="MLGEDAYEKANDWTIGSNQSSFTRRTFEEGVAVTFTNPIFGGIP
                     CK"
     gene            complement(2297..>2616)
                     /locus_tag="PKH60_04980"
     CDS             complement(2297..>2616)
                     /locus_tag="PKH60_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17424.1"
                     /translation="LAANPAFQIGRESGQSNNKKRIIAQIYAKQEELEEAKQRIDKLE
                     KLVERVSEEVKELRNYTQNNGIKPDKLRKIAQNNDENGRANTILQSQRRKGKKKKERP
                     STK"
BASE COUNT          654 a          586 c          568 g          808 t
ORIGIN      
        1 aaaggaaagc atttgtaact ataggacacg atataatgga gaataggagt tacgcagcat
       61 tcgccgcgcc ttgataattg agtatttcaa aagccgaatt caatgagagc aagactctat
      121 caaaggaaga tgcttgcaaa gatacaggga ttgtatctgt ttttcgtaca tcaagaagac
      181 agaatttgcc ttgctttttt accaacaaga gcgaggaaag caagcttttc ttttcaccga
      241 tggtttgtgc aatagtgccg ttgaaagtca aatcaggctg taattcaagc accacaaacg
      301 caaacaaaga aaacgcgaga tggccttgct gcattgtcag agagcgggct tgacaagcct
      361 cgaaattgag attttgtttg atatctcgaa acatagtttc gatcttccaa tcgcaagcaa
      421 taaaattgtg attgttcaaa aatttttttt tgcgaagata gagcagcctc cgattcgctg
      481 taactagaag ttggcaacag gtggtagaca tcaacatcgc cattggaaaa taccactact
      541 ctctcaataa gcaactgaac cagtttttgg cgatcttcaa aagacagccg atccagacat
      601 aagcccaggt tttggtgaaa ctgctgtaaa tccatttcta ctttttgcca gtgtaatgag
      661 cgctgctgtt gcaatttcaa gtgctgacgt tctttgtcaa gagcagcttg ctgctcaagg
      721 agttgctgtc tgcgtttggc caattctgac aactcaataa gtccttgttg ataggcatcc
      781 aacaagcgtt cgagttgtcg ttgcagccgt tgaagttgag tatcgacccg cgccagttgt
      841 gcctgaaact gattcaaatc accctcttgc agttgctggc tcagttgata ttcatgtgcc
      901 agagtttgtg gttgctgtaa tagttggtat aaagattgcc agaccaactg gtcgagacga
      961 tcggctctta cgcgccgaga aggacagcgt tggggacgac caacggtaac tgggtctttg
     1021 ccgacacagc aatagtaaag gtactcatac tgcccaccaa cgctcaattg atgggtaccc
     1081 atcataaata gaccgcactc gccacagcgt accaagctgc gtaacagata acgtctcgaa
     1141 ctcggttggt aaatcctagc agcttggcgg cggttttcgg ctaactgtat ttgcgctttg
     1201 tcaaaggtct cttgagaaat gatggcaggt gctgtcgtct caatccattc ctctaccggc
     1261 cttttttctc gagcgttaca agtactggta gtcttaggcg taaacttgcg ccgccggtct
     1321 ttctgagcag tcgctctggt tttatggcaa taaccgtggc cagtcatcaa aggattagac
     1381 aaaatgccac gaaccgtagc agaatgccag acgctgtttt tacctgtttt ggtcggtatt
     1441 ttctgttgat tcaggcgttt ggtaatctgc ctagtagtca gttgctcttc gatcagccag
     1501 gcaaacattt gctgcacgac ttcggcctct tgtggttcaa tctctgcacg tgggggacag
     1561 cccggttgct ttggaatata gcgtagaccg taagctctac aaccccaagg gataaattcg
     1621 cctttgtgcg ctttggctaa ccgacctcga cgagtacgct ctttgatttg ttcgcgttca
     1681 aactcggcaa tgattccctg aacctgtaat aacaggtgtc cttgcggcgt atcttgaaaa
     1741 ggggtctgga caaagataac ctgacaatta tacttcttaa attcttccat cagcagccac
     1801 tggtgtgcat aattgcgtgc caagcgatcc ggtgccatga tgagtacggc atccacatca
     1861 cccatctgaa tgcagtcgcg aagtcgttct aattcggggc gatctaggcg tgttccacta
     1921 atgccattat ccagaaaaat gtactccggt agaacctggt agttgtactc ttgagcatag
     1981 gcttgcagca gcgtcacttg actctctatc gtgtcgtttt tttcctgagt gggtgttgac
     2041 acgcgggcat acagagctac ttgcatggga tacctccaaa gattgggttt gtaaaagtaa
     2101 ccgcaacgcc ttcttcaaac gttcttcttg taaaggatga ttggttggaa ccaatcgtcc
     2161 aatcgttagc tttttcatag gcatcttctc ctaacatgct tctggataag atgcctgata
     2221 actcattata cttcaatcat aattccatta tcttccccat ttatactcct ttgatttagt
     2281 cgcccgtaaa aagatattac ttggtactcg gtctttcttt cttctttttt cctttccttc
     2341 tttgggattg aagaatcgtg tttgctcttc cattttcatc gttattttgt gcaattttac
     2401 gtaatttatc tggttttatc ccattatttt gcgtataatt acgtaattct tttacctctt
     2461 cagacactcg ctctactaac ttttctaatt tatcaattct ttgttttgcc tcctctaatt
     2521 cttcttgctt tgcgtatatt tgtgcaatta tacgtttttt attgttactt tggccagatt
     2581 ctcgccctat ttgaaaagca ggatttgctg ctaagg
//
LOCUS       DAOQZP010000090         2612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_43266_length_2612_cov_4.062964, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000090 DAOQZP010000000
VERSION     DAOQZP010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43266_length_2612_cov_4.062964"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PKH60_04985"
     CDS             complement(<1..745)
                     /locus_tag="PKH60_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HNQ17425.1"
                     /translation="MTNHKSTYRIGLAGSTQHTADLANQLWSDNRFEIRWVLSPGTKF
                     IGRKQEPVDNPLHLWSVEHELPSIRIANKIDQKVKQEIDALQVEVDVLLVVDFGYFVP
                     QWLLELPKVAPLNLHPSMLPEWRGSSPGQAVLLSGAQTSAITLIQMDNQLDHGAVIHQ
                     ESFAVDPSWTQVEYYRAAFAAVTPLIGNLIKQFILGDLIPSPQPAISPTPIARELTKA
                     DSFLTWELVVSLMQSDSKLPVDQFESQLPP"
     gene            complement(753..1328)
                     /gene="def"
                     /locus_tag="PKH60_04990"
     CDS             complement(753..1328)
                     /gene="def"
                     /locus_tag="PKH60_04990"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNQ17426.1"
                     /translation="MMEIITIPHPTLRQKALPVTRVDKKLLTFLKNLGQTLTNTKNPR
                     GVGLAANQVNKKIAVFSTLVATDLSKSPQLRLFINPTIIDRSEQTTIGLSASDKKPFE
                     EGCLSMPKLYGAVPRSSWVKVTFDQVVGNELSTQTEVFENLAARVVQHELDHLLGILF
                     TDYSATTGLPVFKEINPDRWEEVDPKLLTLF"
     gene            1944..>2612
                     /locus_tag="PKH60_04995"
     CDS             1944..>2612
                     /locus_tag="PKH60_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17427.1"
                     /translation="MTENVPAPEEVLAEAQTVMNDNPLGLEYGDIDGQSEAKNPLSSG
                     ELPSANLRLWRTMQRAIRELIDSFDTSKLNDVDTILLQTRILQSDSLLMTWFISSGNL
                     QEMFELKMKSLLERGVCEDEDDMRLQICVDFINKIRQRNKEDQQYQNKYLPTIGIELE
                     FLFIHDPDYPELNFNASDYYYRTNRLKPLDPPYKPIQEVNGAIEYAIGPSASHLTQLR
                     EILHL"
BASE COUNT          787 a          497 c          536 g          792 t
ORIGIN      
        1 acggtggtaa ttgtgactca aattggtcaa cgggtaattt cgagtcagat tgcatcaagc
       61 ttactactag ttcccaggtc aaaaaactat ctgcttttgt cagttcgcgc gcaattggtg
      121 ttggtgatat agctggttgt ggggatggaa tcaggtcgcc gagtataaac tgtttaatta
      181 agtttccaat caaaggtgta accgcagcaa atgcagcacg gtagtattca acttgtgtcc
      241 agcttggatc aacagcaaat gactcttgat gaataactgc tccatggtct aattgattat
      301 ccatttgtat gagtgtaata gctgaagttt gagcgccgct taacaacacc gcttgaccag
      361 ggctactgcc acgccattct ggtaacattg aaggatgcaa gttaagcggc gccacttttg
      421 gtaactccag taaccattga ggcacaaaat agccaaaatc taccaccagc aaaacatcta
      481 cttcaacttg cagggcatcg atttcttgct taaccttttg atctattttg ttcgctatgc
      541 gtatgctggg taattcatgt tcaacactcc agagatgaag tggattatcg actggttctt
      601 gttttctacc aatgaattta gttcccggag atagaaccca acgaatttca aatcgattgt
      661 cagaccaaag ttgattggct aaatcagctg tatgttgggt ggatcctgcc agcccaattc
      721 gatatgtact tttatgattt gtcattgagt acctaaaaga gggttaataa cttggggtca
      781 acttcttccc agcgatcagg attaatttct ttgaaaacgg gtaggccagt tgtggcgctg
      841 taatcagtaa agagaatacc aagtagatga tcaagttcat gttgaaccac acgagccgcc
      901 aagttttcaa acacctcggt ttgagtactt agttcatttc cgacaacttg gtcgaaagtt
      961 acttttaccc acgaagatct tggtaccgct ccgtagagtt tgggcataga taaacacccc
     1021 tcctcaaatg gttttttgtc actcgctgat agaccaatag ttgtttgctc agatcgatcg
     1081 ataatagttg gattgataaa caatcgtaat tgcggtgatt tactcaagtc ggtcgctaca
     1141 agtgtgctaa aaacggcaat tttcttgttt acttggttgg ctgccagtcc cactccgcga
     1201 ggatttttgg tattagttaa tgtttggcct aaatttttca aaaaagttaa caacttttta
     1261 tccactcggg tcaccggcaa cgctttttga cgcaacgtgg ggtgggggat agtaattatt
     1321 tccatcatca gatagatagt ataccgcata cttgagaaaa tagactaggc ctagagtatt
     1381 tatgcaaagt cctataaaat atttgatgaa cgatcggtta tttgaacact ctcttagaat
     1441 tccatcttat taattattta tgactcagaa agatgatcga attgatactg ctgttttctt
     1501 agataaatcg cttcgacctg cagtgcattt atttggagtt acttgggcat tattggtagc
     1561 aagaaagctt gtacattctg atataccact gccaaaactc agatttctca attgctgagt
     1621 gtagaaacct acggagacca ttagctcaaa tatttgccga aatatttaaa gatatgatta
     1681 atagcgaaca agaattgctt ttcaacagtc acgacgtgga tgtcctgagg ttgtttgttc
     1741 gctctgcagg tacattttta cacaaaggat ggacccagac caatataaaa gcagcttgca
     1801 gtttagaaaa cagttgactc aagttgctga atatttggtt ccaattgtaa atgtctaaac
     1861 aaaagatata cctactgttt taaactggtt tccaaattta tcactttttg ttttgatggt
     1921 ggcttacatg ctatgctgaa ttcatgacgg aaaatgttcc agcacctgaa gaagtgttgg
     1981 ctgaagcaca aacagtaatg aatgacaacc cgcttggctt agaatatgga gatatagacg
     2041 gccagtcaga ggctaagaat ccattgtcat ctggcgagct accttcggct aacttaagac
     2101 tttggcgaac aatgcagaga gcaatacgcg agcttattga cagtttcgat acgagtaaac
     2161 taaacgacgt cgataccatc ttgttacaaa cacgaatttt gcaatcagat tctcttttaa
     2221 tgacttggtt tattagttct ggtaatttac aagaaatgtt tgaattgaaa atgaaatcac
     2281 tccttgagag gggagtgtgt gaagatgaag atgacatgag attgcaaatc tgtgttgatt
     2341 ttataaataa gattaggcaa cgaaacaaag aggatcaaca gtaccaaaat aaatatttgc
     2401 cgaccatcgg tatcgagttg gaatttcttt ttatacatga tccagattat ccagaattga
     2461 attttaatgc ttcagattat tactatcgaa ccaacaggtt aaaaccatta gatcccccat
     2521 acaaaccaat acaagaagta aatggcgcta ttgaatacgc cattggacca agcgcatctc
     2581 acctcacaca attgagggaa atacttcatc ta
//
LOCUS       DAOQZP010000091         2597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_43671_length_2597_cov_5.016129, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000091 DAOQZP010000000
VERSION     DAOQZP010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43671_length_2597_cov_5.016129"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="PKH60_05000"
     CDS             <1..1295
                     /locus_tag="PKH60_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17428.1"
                     /translation="NYIDSGVIQFLNPPQAFAKLLFFTSLILLRDFWQKKSWKLAVIL
                     GVMIASMISLKIYFALFLGLGLAFVYLLEICKVLVQIIRKKSTISKSWLNYRWDLVLG
                     LTIFGIGAVLYLPSNSQAGGIYFDFLFWPRLLLGQGKIFWNEWWLRLQVYEAAQNTRA
                     LFVWYSLAIGVFLSAIYHFRLVGLLVVFKRFRSKMMGKEVILLAVPSIALTHIAMNYV
                     QISGGGNIFNFFVVAITSLSLLSTIVLGELWQQIPKLRFLIVICVGIMAIQPLYNTYA
                     YLKNYYYRRDKIVITAQHEALMSYLSANDESENAAKKYLQTSPGAFLDNNTPYWYFFT
                     GKLSYFSGRQILQDHNQDVSAKKQVEQFIFLDPATASDSARIAHTAHIGQIVLQRSQK
                     STDAFLFRAKQATFSSTFIWQQEYENEDWLVLRPVPMD"
     gene            complement(1292..1972)
                     /locus_tag="PKH60_05005"
     CDS             complement(1292..1972)
                     /locus_tag="PKH60_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17429.1"
                     /translation="MNELTNIAQLLQELPLFYVGVGVLFVLLFVTKNNFTQPANIPHK
                     DHTSILALFTLFFAIIPAVYFGWGFYQAYFPDLKYDEVAGSVDFHVYQPQYLPPKIQQ
                     ASAFHQRENADFNTSPTVRTHYGKNISQLIEDEKSQIIVIDQSKTPTPFSLLAFIESR
                     TEESESQVTIDPIQIPSFPNTPAYLVTNSIVSTVWMHTNDGVLISVTSQTLRTPTEEL
                     IKIAESLR"
     gene            complement(1977..2525)
                     /locus_tag="PKH60_05010"
     CDS             complement(1977..2525)
                     /locus_tag="PKH60_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17430.1"
                     /translation="MPSKTLLDYITTEQKKGISEAAIRTQCLGAGWPTAMIDEAFAFQ
                     NSIKNGPGSPTAVSLKKRSKLLGFISLGFNILSLLYAAKIVMLAGTVVIMQRALVKNV
                     DGSLSFEFLTYNGWFYPVVFGLSFIASYLAITTSLMLPLRTKSVWNKAVISLICILFI
                     ELVIQYLSIQFILPINHLSNSY"
BASE COUNT          782 a          462 c          575 g          778 t
ORIGIN      
        1 ataactatat tgatagtgga gttatccaat ttttaaatcc acctcaggcc ttcgctaaac
       61 tcctattttt tacgagcttg attttattac gagatttttg gcaaaaaaag agttggaaat
      121 tagctgtgat tttgggcgtg atgatcgcgt cgatgatatc ccttaaaata tactttgcgt
      181 tgtttctggg cttgggactg gcgttcgtgt atctcttgga aatctgcaaa gtgctcgtac
      241 agataattag aaagaaatca accatctcaa aaagctggtt aaactatcgg tgggatctag
      301 tgctggggtt aaccatcttc ggaatcggag cagttttgta tttaccaagc aactctcagg
      361 caggcggaat ctattttgat tttctatttt ggccaagact gttactcggt cagggaaaaa
      421 ttttctggaa tgagtggtgg ctccgtttgc aggtgtatga agcagcgcag aatacaagag
      481 cgctctttgt atggtattcc ctggcaattg gagtattttt atcggcgatt taccactttc
      541 ggctggtagg gttgttggtc gtttttaaaa gatttcgtag caagatgatg ggtaaggaag
      601 tgatactcct agcggtgcct tcgattgcac taactcatat cgccatgaat tatgtgcaga
      661 tctctggtgg aggcaacatt ttcaattttt ttgtcgtagc gattaccagc ttgagtttgc
      721 taagcactat cgtgctgggt gagctatggc aacaaatacc aaagttgaga tttctgatag
      781 tgatttgtgt tggaataatg gcaattcaac cgctgtataa cacctatgca tatctaaaaa
      841 actattatta ccgaagagat aaaattgtga ttaccgctca gcatgaagca ctgatgagtt
      901 atctttcagc aaatgatgag agtgaaaatg cggcaaaaaa gtatctacaa acaagcccag
      961 gtgcgtttct agacaacaac acaccatatt ggtatttctt caccggcaaa ctcagctatt
     1021 ttagtggcag gcagattctg caagatcaca accaagatgt gagtgctaag aaacaagtcg
     1081 aacaattcat ctttcttgat cctgcaactg caagtgactc agcccgcatt gctcatacag
     1141 cacatattgg ccaaattgtt cttcaaagat cacaaaaatc gactgacgcc tttctttttc
     1201 gagccaaaca ggccaccttt tctagcacct tcatttggca acaagaatat gaaaatgaag
     1261 actggctggt gctcaggcca gtaccgatgg attagcgcaa tgattcggcg attttgatca
     1321 gttcttcggt aggggttctc aaagtttgac tagtgacgct aattaatact ccgtcattgg
     1381 tatgcatcca aacagtcgaa acaattgagt tagtaaccag gtaggctggc gtattgggaa
     1441 atgaaggaat ctgtatggga tcgattgtaa cttgcgattc agattcttca gttcgtgact
     1501 ctataaacgc aagtaatgaa aaaggagtgg gtgttttact ttgatcgatc acaatgatct
     1561 gacttttctc atcctcaatt aactgtgaaa tatttttacc atagtgagtt ctaacggttg
     1621 gagaagtatt aaaatcagca ttttctctct ggtggaacgc agaagcctgc tggatttttg
     1681 gtggtaaata ctgtggttgg taaacatgaa aatcaacaga gcctgccacc tcatcatatt
     1741 ttaaatcagg aaagtaggct tgataaaacc cccagccaaa atagacagct ggaatgatag
     1801 cgaagaaaag cgtgaataag gctagaatcg aagtatggtc cttatggggt atattggctg
     1861 gttgagtaaa attattttta gttacgaaga gtagaacgaa aagcacgcca acgccaacat
     1921 aaaatagtgg tagttcttgt agcaactgag caatgttagt aagttcattc ataagattaa
     1981 taagagtttg ataaatgatt aattggcaaa ataaattgga tagacaagta ctgaatgact
     2041 agctctataa ataagatgca gatgagagag atgactgctt tgttccaaac ggattttgtt
     2101 ctaagcggca acatcaagga tgttgtgatt gctaagtagc tagcaataaa agaaagccca
     2161 aaaacaactg ggtaaaacca accattgtag gtgagaaact caaaggagag tgagccatca
     2221 acatttttta ctaaggctct ttgcataatt acaacagtgc ctgctagcat cactatcttt
     2281 gctgcgtaga gcaagcttag tatattaaat ccaagactaa tgaatcctaa aagcttactt
     2341 ctttttttta atgaaactgc tgtaggggag ccaggaccgt ttttgattga gttttggaaa
     2401 gcaaaggctt catcaatcat ggcagttggc caacctgccc ccaagcattg tgtgcgaatt
     2461 gctgcttctg aaataccttt tttttgttca gtggttatat agtcgagtaa ggttttactt
     2521 ggcatagcgg gcgagcaaca actttcattg tacagtataa tcagtcttca tagggctatg
     2581 atgtaatggt agcatta
//
LOCUS       DAOQZP010000092         2581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_44098_length_2581_cov_6.250198, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000092 DAOQZP010000000
VERSION     DAOQZP010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44098_length_2581_cov_6.250198"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PKH60_05015"
     CDS             <1..788
                     /locus_tag="PKH60_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17431.1"
                     /translation="IVLDTLNDTETIIQLNKGRIVVQLPEGSSQGKSFRVKSTDGSQA
                     KIIGSIMGVIYDSEASYFRMDCFEGTCYSSTDDEVWDSISVGWHIILSPLEQREKSIG
                     TLNEFWVFVPQLIPTPTAIIIPTEEPTMLPSEKFTPAGQDFYTPQPGYTQPAHAISTP
                     TFTVTVTTPTNTATITTSAFPVLPFTPTKSHTPTPSNTPSPTQSPSPSLSPTNTFTPR
                     STNTKAPTLTQTLTLTPSPTSTFTPTFTYTFTPTPTKTKNNSD"
     gene            927..2360
                     /locus_tag="PKH60_05020"
     CDS             927..2360
                     /locus_tag="PKH60_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17432.1"
                     /translation="MNLSVNFGKDQQPGLKNKNFEGTDGVFFKEINNSAGKATLKLGI
                     VVDLIQSTNLGMIPADVFENSVQILEAHPYKNLQSALSEVKSRIIQFLTKESLGDSIS
                     FSLVAIENSTLHTISLGNRTRVYVFSKGKVREVSTGLEQAPEDVFDVNSGYSKVALTK
                     GDKIILCTDGLFTMLQNQEELTIIDRYDQPSFGAKHLNSLAKGRNVEDSVTTGVINYG
                     ESQSIKKLPLFVLAGLILLAIVLFLIFPKQETPLPEDLGVAIVTSGKVFAQDNLGEFS
                     IAIESLGIVDPGTFLVVQNGEPAEIKLKTRTETFNSTQNISGVTLFASESTIFNIRNI
                     NYANVVNGKTSAADLLNQTTIELVSGKIILINEFSPRVYHIIFSKDNSDWSSTLTLTP
                     VKVPSGIAAIRNGDEIQLFCLSGSCTLTFEDELVEIQENSKVSINGLEKIKDTLTTLS
                     ISQQDLSDWNDITDGYLYAVFLDYLPK"
BASE COUNT          817 a          528 c          467 g          769 t
ORIGIN      
        1 aaattgtact ggacaccctg aatgatacag agacaattat ccaactcaat aaaggcagga
       61 tcgttgtgca actcccagaa ggttcttctc aagggaaaag ttttcgggtg aagtctacag
      121 atggtagtca agcaaagata atcggctcga ttatgggagt aatttatgac tcagaagcga
      181 gttacttccg aatggattgt tttgaaggaa cctgttattc ttccactgat gatgaagtgt
      241 gggattcaat ttcagtaggt tggcatatta tcttatcccc attagagcaa agagaaaaat
      301 cgatcggtac cttgaatgag ttttgggtgt ttgttcctca acttattcca acgccgacgg
      361 cgattatcat accgactgaa gaaccgacga tgttgccctc ggagaaattt acgccggcag
      421 gtcaggattt ctatacaccg caaccaggtt atactcagcc ggctcatgcc ataagtaccc
      481 caacatttac cgtcaccgta acgactccga ccaataccgc gacgataaca acttcagctt
      541 ttcccgtact acccttcaca cctacaaaat cgcacactcc taccccctcg aatactcctt
      601 cgccgacaca atccccttcc ccttcactta gtcctaccaa cacttttact cctaggtcga
      661 cgaatacaaa ggccccaaca cttacacaaa ctctaactct aacaccatct ccaacttcaa
      721 catttacacc cacttttact tatactttca caccgactcc gacaaaaacc aagaacaatt
      781 ctgattaatt gcgatttgac cttaactaca tggtgaatat agattttttc agaaatacaa
      841 tttgacttgg agttttataa gcaataatac tttttacttt gatgaataat gactgaaatc
      901 ttacacctat gataggatga aacttcatga atttaagcgt aaattttggg aaagaccaac
      961 aacctggctt aaaaaataaa aatttcgaag gaacagatgg cgtatttttt aaagaaatca
     1021 acaactctgc tgggaaagca actctaaaat taggcattgt tgtcgatctt attcaatcca
     1081 ctaatcttgg aatgattcca gcagacgtct ttgaaaattc agtgcaaatt cttgaagctc
     1141 atccatataa aaatcttcaa tcggccctca gtgaagttaa aagcagaata atccaatttt
     1201 taaccaaaga atccttgggt gattcaattt cattttccct tgtcgcaatt gaaaactcga
     1261 cgcttcacac aatttccttg ggaaatcgga caagagtcta cgttttcagc aaaggtaagg
     1321 ttcgagaggt ttcaacagga ttggaacaag cacctgagga tgtttttgat gttaacagcg
     1381 gctattcaaa ggtcgctttg acaaagggtg acaagattat cctttgcacc gacgggcttt
     1441 ttactatgct gcaaaatcag gaagaactga ctattatcga ccgctatgat caacccagtt
     1501 ttggcgcgaa gcatttaaat tccttagcta aagggaggaa tgttgaggat agtgttacga
     1561 caggagtcat aaattatggc gagtctcaaa gtataaaaaa gctcccgctt ttcgttcttg
     1621 ctggtctaat attgctggca attgttcttt tcctcatctt ccccaagcag gaaacgccac
     1681 tgcccgaaga tttgggcgtg gctatagtca catctggtaa ggttttcgca caagacaatt
     1741 taggtgagtt ttcaattgca atagagagct tggggattgt tgatcctggg acttttttgg
     1801 tggttcaaaa tggtgagcca gcagaaataa aattaaagac gagaacagaa acatttaatt
     1861 cgacccaaaa tatttcagga gtaacactat ttgcatctga atctacaata tttaatattc
     1921 gtaacattaa ctatgcaaat gtcgtcaatg gtaaaacttc tgctgctgac ctattaaatc
     1981 aaaccactat agaattagtt agtggaaaaa ttatccttat caatgagttt agcccgcgag
     2041 tttaccacat tatattttct aaggataatt cagattggtc tagtactctt acacttacac
     2101 cggttaaagt tccttctgga attgcggcca ttcgaaatgg agatgaaatt caacttttct
     2161 gtctaagtgg ttcatgcaca ttaactttcg aagatgaact cgttgagatc caagaaaata
     2221 gtaaagtgtc aattaatggt ctagaaaaaa ttaaagacac tctaactacc ctttccattt
     2281 ctcaacaaga tctatccgat tggaacgata ttacagatgg gtacctgtat gcagtttttc
     2341 ttgactattt gcctaaataa ttttatatgg atgaggctat ttacttttgt agaagtaaaa
     2401 agttctccaa ggctgttaga aacaatctaa taagagaaaa ttagggcctt tttgttctgc
     2461 tcatttagca cataacgatg ttaatactgt taccaaactt atataatgat ctcatagcag
     2521 atcttgtgat agaattttgc atcgtacaat caatttgaat tgactcatta atcagattga
     2581 g
//
LOCUS       DAOQZP010000093         2572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_44352_length_2572_cov_9.019468, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000093 DAOQZP010000000
VERSION     DAOQZP010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44352_length_2572_cov_9.019468"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..502
                     /locus_tag="PKH60_05025"
     CDS             86..502
                     /locus_tag="PKH60_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17433.1"
                     /translation="MGFAIGQLISQIIGGAMAALLIALIIQWATKKVAKFKPSYGMAY
                     KSAFLGTVGGLILGFMLGFAFGMAGAGEAQQGGLILLSMIAGFLLQGAIYGQLLKDAS
                     SISVGLGKGYAIAGIQFAIGIGILVIITGAVLVIAS"
     gene            529..2511
                     /locus_tag="PKH60_05030"
     CDS             529..2511
                     /locus_tag="PKH60_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNQ17434.1"
                     /translation="MKKIVLFLIAIFFHSYCFSADIKIDSEIISVPAYAGFVEISKIS
                     PDTMAMFKDMLPPTNNLHAVFVTASDSGKLLKSQSPELKRYLIVQTAKSLESVAFGAR
                     HFAELRTKIREEYESLFDQNKSKIDELAKNASQKLSNRISDKFEMKVGEMLPMSINSE
                     TAYHISLSSLTKFSTSTADENTDFISACTMSMVLLKGRVVYLNVFSTYNNLEDLKWTQ
                     SVAEKWTESTVRANQNTQAGFGEIVPAGTRIDPEVKEVLSAEKKTYSTLGHPKSKGLN
                     ISVKYPSVWVAKEGLRPHIVQKFTGSSTGSIVPGCMIIIQELPSIGKIFLDGQMTEEI
                     FSEGVKDYIPPGAVYIDGGTTKIDAEPGAWVKYYFEQETSGVKIGMYSLQYILFYKGS
                     MFALQCSIAGSASEKLILEDAFSSYLPVFQNIGSSIYIPDKWNKEMSNADVASSVMKD
                     TYGENWLLTLLFSAILTWGIGLAPPLLIRFAIIKHPLSKPPTLITVIFLWVFNFVFFT
                     ALGSESKTHAGLLLVAWASYAILRKGSAKYEAEQTRHREEENLKAEEHRRKEEERRRA
                     EEESRRKKDRENEQKEKYNRERKSIKDEEYYQNILGLGSNFTVEDIKRRYRELVAKYH
                     PDKVNHLGEKLKETAEREMKEINEAYEYFKKKYNFK"
BASE COUNT          828 a          499 c          549 g          696 t
ORIGIN      
        1 cacaacaagt aaatccagcg gacgccgtac cggcgccgct gatttctgac gttgggcagg
       61 taaataaaca tcaaggagga gaattatggg ctttgcaatc ggacaactta tcagccaaat
      121 aattggaggt gctatggctg cgcttctgat agcgcttatt attcagtggg ctaccaaaaa
      181 agtggccaag ttcaagccct cttatggaat ggcatataaa agtgcattcc ttggaactgt
      241 tggaggctta attctcggtt ttatgcttgg ttttgctttt ggaatggctg gtgctggaga
      301 agcacagcaa ggcggattaa ttctattaag catgatagca gggttccttt tacagggcgc
      361 aatatatggt caactactaa aagatgcatc aagcatatcc gttggcctcg gtaaggggta
      421 tgcaattgca gggattcagt ttgcaattgg aataggtatt ttggttatta tcacgggggc
      481 cgtacttgtg attgcaagct gaaattcttt ctccatgcga ggtttatttt gaaaaaaatc
      541 gttttatttt taatagcaat atttttccat agctattgct tttcagcgga tataaaaatt
      601 gattcagaaa taatttccgt accagcatat gctggctttg ttgaaatcag taaaatttct
      661 ccagacacaa tggcaatgtt caaagatatg ttgccaccaa caaacaatct acatgctgtc
      721 tttgtaacgg catccgattc tgggaagttg ctaaaatctc aatcccctga attgaaaaga
      781 tacctcatcg tccaaacagc caagtcactt gagtctgttg cttttggtgc acgccatttt
      841 gcagaactgc gtactaagat ccgggaggaa tacgaaagcc tttttgacca aaacaaatct
      901 aagattgacg aattagccaa aaatgccagt caaaaattat caaatcgtat ttccgacaaa
      961 tttgaaatga aagtgggaga aatgcttccc atgtcgatca attctgaaac tgcatatcat
     1021 atttcacttt catctcttac taaattttca acttcaacgg cagacgaaaa tacagacttt
     1081 atttcagcat gcaccatgtc aatggtttta ctgaaaggcc gcgtcgtata cttaaatgtt
     1141 ttctctacat acaacaacct tgaagacctg aaatggactc aaagtgtcgc tgaaaaatgg
     1201 acagaatcca cagttagggc aaatcaaaat actcaagctg gattcgggga aattgtccca
     1261 gctggtacca gaattgaccc tgaagtaaaa gaagttctct cggcggagaa aaaaacatat
     1321 tcaacgcttg gccatccaaa atcaaagggt ttgaatatat cggtcaagta cccttccgtt
     1381 tgggtggcta aagagggcct acgcccacat attgtacaaa aattcacggg gtcatctact
     1441 ggcagtattg ttcctggatg tatgatcatc atccaagaat tgccctcaat cggaaaaata
     1501 tttttagatg ggcagatgac ggaggaaatc ttttctgaag gagttaaaga ttatattcca
     1561 cctggcgctg tatatattga tggaggaact accaaaatag atgcagagcc tggggcttgg
     1621 gtaaagtatt attttgaaca agaaacttca ggcgtgaaga ttggcatgta cagtttacag
     1681 tacattcttt tttataaggg ttcaatgttt gcgttgcagt gctcaatcgc tggttctgca
     1741 tctgaaaaac ttattctcga agacgcgttt tcatcctatc tgccagtatt tcaaaatata
     1801 ggtagcagca tttatattcc tgacaagtgg aataaagaaa tgtcaaatgc tgacgttgct
     1861 tcatcggtta tgaaagatac ctatggagaa aattggttat taacgctctt gttttcggcc
     1921 attcttacat gggggatcgg tcttgctcct cccctactta tccgttttgc catcattaaa
     1981 catccgttat caaagccccc cactttgata acggtaatat ttctttgggt atttaacttt
     2041 gtgtttttta cagcacttgg cagtgagagc aaaacgcatg ctgggctact actcgttgca
     2101 tgggcatctt atgccattct gagaaaagga tcagcaaaat acgaagcaga gcaaacaagg
     2161 catcgggaag aagaaaacct gaaagccgaa gaacacagaa gaaaggaaga agagagaaga
     2221 cgcgcagaag aagaatcacg tagaaaaaaa gacagagaga acgaacaaaa agaaaaatat
     2281 aatcgggaac gtaaaagcat aaaagacgaa gaatactacc agaatattct cggccttggt
     2341 agcaacttta cagtcgagga cataaaacgt cgttacaggg aactcgtagc caaatatcac
     2401 ccagataagg taaaccacct cggagagaaa ctgaaagaga cggctgaaag agaaatgaaa
     2461 gaaataaatg aagcttatga atacttcaaa aagaaatata acttcaagta aatgcccaac
     2521 aaccgcatca actcggactg gcaattccgc tgcgctccat tgccagccgg tt
//
LOCUS       DAOQZP010000094         2558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_44731_length_2558_cov_5.801438, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000094 DAOQZP010000000
VERSION     DAOQZP010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44731_length_2558_cov_5.801438"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="PKH60_05035"
     CDS             complement(<1..992)
                     /locus_tag="PKH60_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17435.1"
                     /translation="MSHSPFIESSGYQIHPHSDSDSFVLSVQEIRTETKKAIANKLAL
                     FEDTTARKEWINKLMQLLPNSIKLESADSDTVRFLTEVASDKSRAQTVLEAIQKPLDF
                     DYLADVDCSLFSELRKNLPVPEQLLALLPNTIQWSIPFPSSAWALVDVLIELGLADEV
                     WERIPKPLPLFEKLSSGFLNILSRFRHQKKYIQEIVAILPPKLDLGSIHFSEYSFLSA
                     IIDLEGVSEVLDRLPDGIDLVTLSHYGAELLIDLCNQHQKKTVISRLPKICNVNEISD
                     AAFHLFSKIDTPRLVLEPRSNSKKISTKLSRVFSSLTKSIEKTNVQPLESISERV"
     gene            complement(1011..1436)
                     /locus_tag="PKH60_05040"
     CDS             complement(1011..1436)
                     /locus_tag="PKH60_05040"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNQ17436.1"
                     /translation="MFFPIVFVLANQHFQLPIVDHAVFQLMGVICITLGSIGWITNIR
                     LFKSTGKGTPVPIEPPKKLVLANHYRHSRNPMYISTLIVLVGYFFLFGHLSLLAYVLF
                     AAIGFHLFVTHYEEPTLKKMFGSKYTAYCKKVPRWIKVL"
     gene            complement(1503..>2558)
                     /locus_tag="PKH60_05045"
     CDS             complement(1503..>2558)
                     /locus_tag="PKH60_05045"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ17437.1"
                     /translation="QSWFMFWLFVLEVPTGAIADKFGRKHSMALGGVVVTIAVLVYAS
                     MPNFAIFLLAEFLFAVAMALISGADSALLYDALKESGNEDQSKKIFGRAHSFHLLGML
                     IAGPVGSLIAAQFGLRAPTLASAIPFLLAAGVAWSIPEPKRGNTASQSPNYLETVRGG
                     LSFLRHHRFARILTLNAILVSAAVYYVVWFYQPVLSQLTIPIVYFGWIHAILVSSEIL
                     VANNFSKIERLFGSERRYLQFTALIVTVSFLLVAIFPNIVTLGIFILFAGGFGLTRIE
                     YITVLLQKEIPSHQRATVLSSISMFRRFALFMINPIVGLLATRSLSLALALVGLLPLL
                     TFLLPKITFPSPEAKTR"
BASE COUNT          818 a          501 c          557 g          682 t
ORIGIN      
        1 actctctcgc tgatggattc cagaggttgc acattcgttt tttcgatgga tttagtcagg
       61 cttgaaaaca cacgagacag cttggtactt attttttttg agttactccg tggttctagt
      121 accaagcgag gagtatcaat tttgctaaac aaatgaaaag cagcatcgct tatctcattg
      181 acattacaaa ttttcggcaa tctgctgata acagtctttt tttggtgttg gttacaaagg
      241 tcaatgagaa gctctgctcc ataatgactt aaagtaacca gatcgattcc atcaggcagt
      301 cgatctagaa cttcagagac tccttccaaa tcgatgatcg cacttagaaa cgaatattct
      361 gaaaaatgaa tagatcccaa atctagcttt ggaggcagga tagcaacgat ttcttgaatg
      421 tatttttttt gatgtcgaaa tcgactcaga atgttcaaga aaccactgct aagtttctca
      481 aaaagcggaa gcggttttgg aattctttcc cagacttcat cggctagacc aagctcaatt
      541 aaaacatcaa ccaacgccca agcagaagat gggaagggta ttgaccactg aattgtattt
      601 ggaagtaatg ctaaaagctg ttctgggact ggaagatttt ttcttaattc actaaaaagc
      661 gagcaatcta catctgcaag atagtcgaag tcaagtggtt tttgaattgc ctcaagcact
      721 gtttgagcac gagacttgtc agatgcgact tcggttaaaa accgtactgt atccgaatca
      781 gctgactcca acttaataga gttgggcaga agttgcatga gtttgttaat ccactctttt
      841 cttgcggttg tatcttcaaa aagggcaagt ttgtttgcta tagctttttt tgtctcagtt
      901 cttatttctt gtacgctaag gacaaaacta tccgaatctg aatggggatg gatttggtag
      961 ccagagcttt caatgaaagg ggagtgagac atgatgagta ttttttgatt ctataatacc
     1021 ttgatccacc taggaacttt tttgcaatat gcagtgtatt tactaccaaa catttttttt
     1081 aaagtcggtt cctcatagtg agtgacaaag aggtggaatc cgattgctgc aaacagaaca
     1141 tacgcaagta aactcaggtg gccaaatagg aaaaagtatc caacgaggac gataagcgtg
     1201 cttatataca tgggatttct tgaatgccga tagtggttgg ccaatacgag ttttttagga
     1261 ggctcaattg gaacaggcgt gcctttacct gtggatttga ataatcgaat gttggtaatc
     1321 cagccaattg agccaagagt aatgcaaata acacccataa gttgaaaaac agcatggtca
     1381 acaattggca gttgaaaatg ttgattagct agaacaaaca ctattggaaa aaacactagg
     1441 acaaaaaatc cgtacaagaa agccaggaaa acagttttac ttttcgtaat ttcaattata
     1501 tatcaacgag tttttgcttc tggagacgga aaagtaattt ttggtaagag gaaagtaaga
     1561 agcggcaaca aaccgaccag ggcgagcgcg agcgatagcg agcgcgtggc caataagcca
     1621 acaattggat tgatcatgaa caacgcaaat cggcgaaaca tagatataga tgaaagtact
     1681 gttgcgcgtt ggtgagaggg aatttctttt tgcaggagca cagtaatata ttcaattctg
     1741 gtcaatccaa atccaccagc aaataaaata aaaattccca gcgtgacaat attgggaaag
     1801 atcgccacca gcagaaatga gaccgtaact ataagagcag taaactggag atatcgtctt
     1861 tctgagccga acaaacgttc tatttttgag aaattatttg caactagtat ttcactactt
     1921 actaagatgg catggatcca gccgaaataa acaatgggta tggtgagttg agataaaact
     1981 ggctggtaaa accagactac atagtacact gcggcagaaa ctaaaatagc gttaagcgta
     2041 aggattctgg caaaacgatg gtgtcgtaaa aatgacaacc caccccgtac cgtttccaga
     2101 taatttggtg attgagaagc tgtattacct cgttttggtt caggaatgct ccaagccaca
     2161 cctgcagcaa gcagaaatgg gatggcagaa gctagggtag gagctctgag accaaattga
     2221 gctgcaatga gactgccaac ggggccagca atcaacatgc caagcaggtg aaaagaatgc
     2281 gccctgccaa agatcttttt actctgatct tcgttaccgg attcttttaa cgcatcatag
     2341 agcagggcgc tatccgcacc agaaatcaac gccatcgcca cggcaaacag aaattccgcc
     2401 agcaggaaaa ttgcaaaatt tggcattgat gcgtacacta acaccgcaat cgtcacgaca
     2461 actccaccaa gtgccatcga gtgttttctg ccaaatttgt ctgcgatggc cccggttggt
     2521 acttctagca caaacaacca aaacatgaac caagactg
//
LOCUS       DAOQZP010000095         2554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_44822_length_2554_cov_4.797119, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000095 DAOQZP010000000
VERSION     DAOQZP010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44822_length_2554_cov_4.797119"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="PKH60_05050"
     CDS             <1..961
                     /locus_tag="PKH60_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17438.1"
                     /translation="EQLQFEQHLRKVRKFLEKRPLMEWADSRPETILRLILSEIEECL
                     VELLADIVQGKASDKLKTKVESAILHLQSFPLHSEEQYNTEKVISFFSEVYDQLFLIL
                     GFASTAQLDFNFDKVYNHINGQGNRSDVFDRLKLLAAGVLDSNPVAALEEYLIVWGSM
                     ATHQEWQIDSAAIMQMVLDKNEQNYPEKYFKLLQYIITPDGRLVKVPLTKEEFGAAFD
                     HRVRCLRLIRRALKLAKLDRVTGMVPTDHLKYEALILNHQDEKSFRFLAIDLVATLGK
                     NSQVFTFEQLVSIESEMHRVVAGRPFTSGEIYPGTTNEILIAR"
     gene            976..1617
                     /gene="tmk"
                     /locus_tag="PKH60_05055"
     CDS             976..1617
                     /gene="tmk"
                     /locus_tag="PKH60_05055"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004232936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNQ17439.1"
                     /translation="MHETQQGFLITFEGGEGGGKSTQIMRVREKLESAGYDVVLLREP
                     GGTVISEQIRDVVLSAKNTGIAYTTEVLLFQAARAQIYREIVLPSLQAGKVVVMDRSR
                     DSSVVYQGVVRGFGRELIDQLNDISTKNTLPNLTLLLDLPVEVGLQRRAESDKQDRLD
                     MEGHEFHHKVRQAYLDLAKSDPNRWVVVDAAKSLEEVEEMIWQIIEPRLPAKG"
     gene            1639..1953
                     /locus_tag="PKH60_05060"
     CDS             1639..1953
                     /locus_tag="PKH60_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17440.1"
                     /translation="MTDILTRVENLADEDTLNKGQTIYDVSYKQLLWRNFLAGMSRAV
                     GMVVIQFLFFAVIAGFVAQFVLPQVMNLFGGYMNTLNTLQGGAKQTQFSFDSIKELLP
                     TQ"
     gene            1963..2475
                     /locus_tag="PKH60_05065"
     CDS             1963..2475
                     /locus_tag="PKH60_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNQ17441.1"
                     /translation="MEVFIIAALSLDGFLGRSADHSSITWRSKEDSEFFMQKTREAGA
                     VVMGSTTFSTMRRPMPGRKHYVLTSNTQQYSSYDTDQVIGVNIQPKEVVELAKQDGYS
                     QLAVCGGSSVYTQFMQAGLIDTLYLTIEPVLFGQGIKLFNDTLDIKLRLIQTHHLSDQ
                     TIVQELAVVE"
BASE COUNT          757 a          457 c          603 g          737 t
ORIGIN      
        1 agaacaactt cagtttgaac agcacttacg gaaggttaga aaatttcttg aaaaacgacc
       61 actgatggaa tgggcagatt ctcgtccgga aacaatactt cgattaattt tatctgaaat
      121 agaggaatgt ttagtcgagt tactcgcaga catcgttcaa ggcaaagcat cagacaaact
      181 gaaaaccaaa gtagaaagtg ccatactgca cctgcagtcg tttccgttgc attctgagga
      241 gcaatacaac actgaaaaag ttattagttt tttttcagaa gtatacgatc agcttttctt
      301 gattttagga tttgcctcta ctgctcagtt agattttaac tttgataaag tttataacca
      361 cattaatggt cagggcaatc ggagcgatgt gtttgacaga ctcaaattgc tcgctgctgg
      421 tgtgcttgat tctaatcccg tagcagctct tgaagaatat ttaattgttt ggggctctat
      481 ggcaactcat caggagtggc agatagactc ggcagcgatt atgcagatgg ttttagataa
      541 aaacgaacaa aattatccag aaaaatactt taaattactc cagtacatca ttactccaga
      601 tggaagatta gtaaaagtcc ctttaacaaa ggaagagttt ggggccgcat ttgatcatcg
      661 cgttagatgt ttacgattaa ttaggagagc ccttaaattg gcaaagcttg atagggttac
      721 agggatggta ccaacggatc atttgaagta tgaagcacta atactcaatc atcaggatga
      781 aaaaagcttc agatttctgg ccattgattt agttgctaca cttggtaaga atagtcaggt
      841 gtttacattc gaacaattag tctcaattga aagtgagatg catcgagtgg tagctggaag
      901 gccgtttact tctggagaaa tataccccgg tacaactaac gaaatactta ttgctcgcta
      961 gaaaggataa aaataatgca cgaaacacaa caaggttttt taattacttt tgaaggtggt
     1021 gagggtgggg gaaaatccac gcagattatg cgagtgagag aaaaactgga atcggctggc
     1081 tatgatgtgg tgttgcttcg tgagcctggt ggcacagtaa tcagtgagca aatccgggac
     1141 gtggttttgt ctgccaagaa tacggggata gcctacacca cagaggtgtt gttatttcag
     1201 gcggcgcgag cgcagattta ccgcgagata gttttgcctt cgttgcaggc cggtaaagtg
     1261 gtggtgatgg atcgctcgcg cgatagtagc gtggtttatc aaggtgtagt gcgtggtttt
     1321 ggccgtgagt tgattgacca actcaatgat atttctacta agaatacttt gccaaatttg
     1381 acgctcttgc ttgatttacc tgtggaggtt ggtttacaac gaagagcaga gagtgacaag
     1441 caagaccgac tcgatatgga gggtcatgaa tttcaccata aagtacgtca agcttattta
     1501 gatctggcaa aatcagatcc caaccgctgg gtggttgttg atgccgctaa atcgctagaa
     1561 gaagttgagg aaatgatttg gcaaatcatt gagccgcgct tgccagccaa gggctagctc
     1621 tgtcatactt gttgcactat gacagatatc ctaactcgag tggaaaattt agctgatgaa
     1681 gacactttaa ataaaggtca gacgatttat gacgtcagct acaaacaact gctttggcgt
     1741 aattttttag ccggtatgag tcgggcagtt ggcatggtgg tgattcaatt tctctttttt
     1801 gcagttatag ctggatttgt ggcgcaattt gttttgcctc aagtcatgaa tttgtttggt
     1861 gggtatatga atacgctcaa cacattgcaa ggaggagcca aacagactca attttcattt
     1921 gatagtataa aagaattact gccgactcaa tagggaggct ctatggaagt atttattatt
     1981 gccgcattaa gtcttgatgg ttttttagga agatcagcgg atcattcttc cataacttgg
     2041 agaagcaagg aagatagcga gttttttatg caaaaaactc gagaggctgg cgcagttgtc
     2101 atgggttcca ctactttttc aacaatgaga cgaccaatgc ctggtaggaa acactacgtt
     2161 ttaacatcaa atactcagca gtattcatca tatgataccg accaagtaat tggtgtaaat
     2221 attcagccaa aagaggtggt tgaattggca aagcaagatg gatattcgca acttgcggtt
     2281 tgcggtggtt cgagtgttta cacgcaattt atgcaagctg gtttgatcga taccttgtat
     2341 ctcacaatcg agccagtact ttttgggcaa gggatcaagc tttttaacga cactcttgat
     2401 ataaaactac gtttaattca gacccatcat ctttcagatc aaacaatcgt acaagaattg
     2461 gctgtagtag aatgagcgct atggtcgagc cctcccaact agtgctaggg ataaaacgct
     2521 ttgatgtaac tctgccgttg cctgaatata aaac
//
LOCUS       DAOQZP010000096         2495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_46489_length_2495_cov_7.393443, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000096 DAOQZP010000000
VERSION     DAOQZP010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46489_length_2495_cov_7.393443"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..427
                     /locus_tag="PKH60_05070"
     CDS             68..427
                     /locus_tag="PKH60_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17442.1"
                     /translation="MPRLVIHTATGPTEVKTKDGSVWICRCGLTENEDGTCSGNHKKL
                     QVADEDPNKIYGYGEDGKRHQLAEEGCGDDCCKDEDHDNDDDDEDDERDDTKEDETEE
                     KQKGCCGGCGGCCREEK"
     gene            complement(504..1988)
                     /locus_tag="PKH60_05075"
     CDS             complement(504..1988)
                     /locus_tag="PKH60_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17443.1"
                     /translation="MRKRLPLLILLTIYVVYSLLSFKSFGITNDEPEDYLLGSFLYTK
                     LRADDPVLERSFVIAEDGAINLAMYDRIYMALQYAISGEINYDTYHLLNLLFFSIILI
                     AAYELLLKETGRTSLATIGPIALFLTPRLFGDIAANHKDVPFAISYFVSLVAIYLFGN
                     KNSAKYLLILGLFFGLTQSLRTVGYSIYLVYVLWLLFNRATLVSFKKIFEILAQVAVI
                     FFIGLLIHFVTLPYLAADPFSRFAELLTNAKAFPWNNAVLYFGTQFLSTELPWHYVPI
                     WFLITTPILFLVYGLLSVKLISKSKLARLLWLSLFVNSVLLVLSQPVLYDGVRHLLFL
                     LPPFVLLACLSFFALLKSKWRWKKIALSLTALNVVLILVQYSLLFPFHYTYFNEIVGY
                     LPGANNQFELDYWGASYKSAVEQFNQRAQFEKTYQLYFCGDPQQVKAYARFKYELVYE
                     INNADLLICHRRGNNQTTIDTFDSKNQFAIKRLGVPLTTVKWTP"
     gene            complement(2098..2493)
                     /gene="rplL"
                     /locus_tag="PKH60_05080"
     CDS             complement(2098..2493)
                     /gene="rplL"
                     /locus_tag="PKH60_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNQ17444.1"
                     /translation="MSEEKLSAKLQKIVDAVKELSLIEVSDLVKAMEEEFGVSAAAPM
                     MMGAMPATASAEPVEEQTEFDVILKDGGANKIGAIKAVRTLKQELGLGEAKTFVESAP
                     KTVLEGVNKAEAEAAKKTLEEAGCVVELK"
BASE COUNT          799 a          465 c          572 g          659 t
ORIGIN      
        1 ccaaatgaaa cggggataaa tacctgtata ctatctgaat cttttactaa taaaataggg
       61 agcacatatg ccacgattag ttattcatac cgccacaggt ccaactgaag tcaaaacgaa
      121 agatggttct gtgtggattt gtcgctgtgg tttaaccgaa aacgaagatg ggacttgtag
      181 tggcaaccat aaaaaacttc aggttgctga tgaagaccca aacaaaattt atggatatgg
      241 tgaagatggc aaacgtcatc aattggcaga agaaggttgt ggggatgatt gttgcaagga
      301 cgaagatcat gacaatgatg atgatgacga agatgacgaa cgtgatgata caaaagagga
      361 tgaaacagaa gaaaaacaaa aaggttgctg cggaggttgt ggcggttgtt gtcgcgaaga
      421 aaaataactt ctcgacctat tttcaccata ggtgttaaag ttaattatca taagtaaagt
      481 gataggccaa aagtaaagta ttcctacggt gtccacttga cggtggtgag gggaactccc
      541 aatcttttaa tggcaaattg gttttttgag tcgaaagtgt caatggttgt ttggttattg
      601 ccacgtcgat ggcagattag taagtcagca ttatttattt cgtagactaa ttcatattta
      661 aagcgagcgt atgctttgac ttgttgcgga tcgccgcaga agtaaagttg ataggttttt
      721 tcaaattgag cacgctgatt gaattgttcc accgcagatt tgtaactggc tccccaataa
      781 tctagttcaa attgattatt tgctccaggc aggtagccga caatttcgtt aaagtaagta
      841 tagtgaaacg gaaagagtag actgtattgg acgaggatga ggactacatt gagtgcggtc
      901 aggctgagag cgattttttt ccagcgccac ttgcttttga gcaatgcaaa gaaactgaga
      961 caggctaaca gtacgaacgg aggcaataaa aacagcaagt ggcgcactcc gtcatacaaa
     1021 actggttgag atagaactaa taatacgcta ttaacaaata gcgaaagcca taataaccga
     1081 gccagtttgg attttgatat tagtttgacc gacagcaaac catagactaa aaaaagtatt
     1141 ggcgtggtga tcaaaaacca aattggcaca tagtgccaag gtaattctgt agacaaaaat
     1201 tgtgttccaa agtataaaac agcgttgttc cagggaaaag ctttagcatt agtaagcagc
     1261 tcagcgaagc gagaaaatgg gtcggcggca agatagggca aagtcacaaa gtgaattagg
     1321 aggccgatga aaaagataac agcgacttgg gctagtatct caaatatttt tttgaatgat
     1381 actagtgttg ctcgattaaa tagtaaccaa agaacataca ccaagtaaat actatatccc
     1441 actgttctta aagattgagt cagaccaaaa aacaggccga gaatcaacaa atatttcgct
     1501 gaatttttgt ttccgaacaa gtaaatagcg actaacgaca caaagtagct aatcgcaaat
     1561 gggacatctt tatgattggc ggcaatgtca ccgaatagcc tgggtgttaa gaatagggca
     1621 attggtccga tcgtagcaag agaagtccga cccgtttctt tgagcagaag ctcatacgcc
     1681 gcaatcaaga tgattgaaaa aaataacaaa ttgagcaggt gatacgtgtc gtaattaatt
     1741 tctccagaaa ttgcatactg caatgccata taaattctgt cgtacatagc taagttgata
     1801 gcgccgtcct ctgcaatgac aaaactacgc tccaatacag ggtcgtcagc acgcaacttc
     1861 gtgtacagaa aagaccccag taaataatct tctggttcgt cgttcgtaat accgaagctt
     1921 ttaaaactca gaagagaata cacaacgtag attgtcaata gaataagtag gggaagtctt
     1981 tttctcacaa aattattgta tcaagtatgt tgtgggagtg agagtttaaa aacccagccc
     2041 caccgttcgc gaacggcggg gctgggaaac agatcaattt caaaatcgat caaaagctta
     2101 cttaagctca acgacgcagc cagcttcttc caaggtcttc ttggcagctt cagcttcggc
     2161 tttgttgact ccttccaaaa cggtttttgg agcggactca acgaaggttt tggcttcacc
     2221 taaaccaagt tcttgcttga gtgtgcgaac tgctttaatt gcaccaattt tgttggcgcc
     2281 accatctttg aggatgacat caaactcagt ttgctcttct actggctcag cactagctgt
     2341 tgctggcatg gcgcccatca tcatgggggc ggcggcggaa acgccaaatt cctcttccat
     2401 cgctttgact aagtcggaaa cttcaattag tgaaagttct ttgacagcgt caacgatttt
     2461 ctggagttta gcggaaagtt tttcttcgga catag
//
LOCUS       DAOQZP010000097         2463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_47463_length_2463_cov_4.079734, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000097 DAOQZP010000000
VERSION     DAOQZP010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47463_length_2463_cov_4.079734"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..>2463
                     /locus_tag="PKH60_05085"
     CDS             165..>2463
                     /locus_tag="PKH60_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17445.1"
                     /translation="MRKTVSIFALLGFFLLSNLKVFAASHPNLVVNQTDISQIRARIQ
                     SAATAQHAFDAMRSQTWINGAPAWAINEGWSRQLVEQSFVGLVDNDSQAINRAKANFL
                     IHTQGVDVYHFSKFNAEAQKYQYGQPCTALVLGYDLLEPHLSALEKSNAQAFLTEWGT
                     GLYQFYSGDPKMVSHNFHTSSIACLGLLGLALDGEVDAASTWSTFATAAFRNFYVNYA
                     FNLGGDYTEGYVYQQYGMASAVMYLDALRRVKNIDLVTESNVANLWNFYLSAYGTDNT
                     FPKYGDNTDGPFIAGTDLYFLNKAKQQGLRGEYLWLWYRLRGSLAAQNLKPYLFKEFD
                     HMAMVLYFPTKVISKVPTSQAFPPSQLLESVTTGTTQGATDKPGGMVVLRSDWNTSQF
                     STSLWLNNRWRWQNHQHYDPNSFTLEGYGETLISNLNNRSYDDPLRGKWSQQNTIRID
                     PNLSNSDAPISPFATGLASSLGTFTRFFRSDFADVVISDSRYSHANLHTFGLPNGNFS
                     LATTANIVPIEKAFRTVLLVRNLLPMPFYLLFDDFKKVGATEYWWQVYIPNTATQIFG
                     QGTLIQPYTYTVGSAIMRMSFLNYPQVQLVRGPQDASRGDRPLQLKIQNSELELLSLL
                     FPSRASDPAPQFRQVQTSPVVYEVSLEGQTVQVLFNPSLSSVVYQDVETDAQIAVGKD
                     WTKPSRQLVFHQATYARFGGADFFRTPQRSTQTVPGAYNHCAKSADFNGDGATSLADF
                     SLLLPRLGEVVSATNEKFDLQCDNAI"
BASE COUNT          678 a          486 c          545 g          754 t
ORIGIN      
        1 tggaagtgac gcgagatgtg tatgactatg gttttcaaaa aggtcatgtt aaatttcgta
       61 acaaatggaa tcgattaatt gaatgtccgg tagatttttc atttactgcc caaaagaatg
      121 ctggattaca gatgtctttg ttgtagttgt gttaggatga atgggtgaga aaaacagtat
      181 caatttttgc acttcttggg ttttttttgc tcagcaatct caaggttttt gcagcttctc
      241 atcctaactt ggtggtcaat cagacagata tttcgcagat tagagctaga attcaatctg
      301 ccgcaaccgc gcaacatgca tttgatgcta tgcgatctca gacttggatc aatggagcgc
      361 cagcttgggc aatcaatgaa ggatggtcaa gacagttggt tgagcagagt tttgtaggtt
      421 tggttgataa cgatagtcaa gcaatcaatc gggcaaaagc aaattttttg attcatactc
      481 agggggtaga tgtctaccat ttttcaaaat ttaatgcaga agcgcaaaaa tatcaatacg
      541 gccaaccatg tactgcgctc gtcttgggat atgaccttct cgaaccccac ctatctgctt
      601 tggaaaaatc gaatgcacag gcatttttaa ccgaatgggg tactggttta taccaattct
      661 attcgggaga tcccaagatg gtttctcaca attttcacac gagctccatt gcttgcttgg
      721 gattacttgg attggcattg gatggagagg ttgatgccgc atcaacttgg tctacttttg
      781 ctacagcagc ttttcgaaac ttttacgtaa attatgcctt taatctgggg ggtgactaca
      841 cggaaggcta tgtctaccaa caatatggca tggcttctgc cgtgatgtat ttggatgcgc
      901 tgaggcgggt taaaaatatt gatttggtga ctgaatcaaa cgtggccaat ctttggaatt
      961 tttatctttc agcgtatgga accgataata cgtttccaaa atatggtgat aataccgatg
     1021 gaccgtttat tgctggtaca gatttatact ttctaaacaa ggcaaaacaa caaggattac
     1081 gaggggagta cttatggcta tggtatcggt tgagaggttc tttggctgca caaaatttaa
     1141 aaccatattt atttaaagaa tttgatcata tggccatggt attgtatttt cctactaagg
     1201 ttatctccaa agtgccgacg agtcaagctt ttcctccttc tcaactactt gaaagtgtta
     1261 cgactggaac tacgcaggga gcaaccgata aacctggcgg catggtggtt ttgcgctcag
     1321 attggaacac gagtcaattt agtacttcac tgtggctcaa taatcgctgg cgttggcaaa
     1381 accaccagca ttacgatccc aactcattta ctttggaggg ttatggtgaa acgctgattt
     1441 caaatcttaa caatcgctcc tacgatgatc cgttacgggg caaatggagt cagcaaaata
     1501 ctattcggat agatcccaat ttgtcaaata gcgacgctcc tatttcacct tttgcaacag
     1561 gattagcgtc atccttaggc acttttactc ggttttttag atcagatttt gctgacgtgg
     1621 tgattagcga tagtcggtat tcccatgcta atctccacac gtttgggttg ccaaatggca
     1681 atttttcatt agctactact gccaatattg tgccaataga aaaagctttt cgtacagttc
     1741 tgttggtgag aaatctgtta cctatgccat tttatttact gtttgatgac tttaaaaaag
     1801 taggagcaac agagtattgg tggcaagtat atataccaaa tactgctact caaatttttg
     1861 gtcaaggaac tttgattcag ccatatacct acacggtggg tagcgctatc atgcgaatga
     1921 gttttcttaa ttatcctcaa gtgcagttag tcagaggacc tcaggatgct agtcgtggtg
     1981 accgaccact acagttaaaa attcaaaatt cagagctaga gctactctcc ttactttttc
     2041 ccagcagagc aagtgatcca gcgccacagt ttagacaggt tcagacctct ccagttgtgt
     2101 atgaagtttc actcgaaggt caaacagttc aagtattatt caatcccagt ttgtcctcag
     2161 tggtctatca ggacgtagaa accgacgctc aaatagctgt gggaaaagat tggactaagc
     2221 caagccgtca actcgtgttt caccaagcaa cctatgctcg ttttggtgga gcagattttt
     2281 tccgtacccc tcaacgctcc actcaaactg ttcctggagc gtacaatcat tgtgcaaaat
     2341 cggcggattt taatggagat ggcgcaactt ctttagcaga tttttcgtta ctgttgccca
     2401 ggctggggga agttgttagt gcaacaaacg aaaagttcga tctgcagtgc gataatgcta
     2461 ttg
//
LOCUS       DAOQZP010000098         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_48242_length_2438_cov_6.322283, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000098 DAOQZP010000000
VERSION     DAOQZP010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48242_length_2438_cov_6.322283"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PKH60_05090"
     CDS             complement(<1..663)
                     /locus_tag="PKH60_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNQ17446.1"
                     /translation="MQIVYLGHSSFKLKGKNGSVVTDPFSQSSVGFSFPSTTTDIITV
                     SHQHADHNAVGLLKPTSRRERPFLVTQPGEYEVGGISVFGVPTFHDDIAGALRGNNTV
                     FTIFLEGIRLCHLGDLGHPLSTEQVEAIGSVDVLFCPVGGVFTLDPATAVKTIRQLEP
                     SIVIPMHYKTAKHEPSIFGELKTVEEFLHTYGVEVEPVTKLEITSLKLPEETEVVVLT
                     PTA"
     gene            738..1202
                     /locus_tag="PKH60_05095"
     CDS             738..1202
                     /locus_tag="PKH60_05095"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17447.1"
                     /translation="MHKETIIAVVIGLLFGLLVTVGVYKLQSVNDSSVATPPLAENIS
                     PSASPSPDDLARLSISYPTQGLISADADITITGKSDPNALLVLFANDTQQISEADDKG
                     AFSLALTLKEGPNFFTIVAANQDGVTFSADRVVVYEKEVPIEVSPEASQSAR"
     gene            1226..2029
                     /locus_tag="PKH60_05100"
     CDS             1226..2029
                     /locus_tag="PKH60_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17448.1"
                     /translation="MNNKTISTRQLFITICFLAIFSCSVSQSVTSISAQTSTKSGSID
                     VRLEGKDIGDGGSATPNPESINKAINERINKQLSQEQEKKLTNIIESINQLRRAIYGK
                     VERVTDDSLTITTADGINSIPLPESVRVKQGTKTIKTSDIAVDNWVLVIGANSVDRSK
                     ETSNSNFTPQQITVFTTNPIAAQPMVELGIIDSITSSSITVISRSSGVSNTFSVLKTT
                     KFQNANGAQTTIDNFEKDLTVLVVGFKGEKSNSATIIRSLATLDKREEN"
     gene            2031..>2438
                     /locus_tag="PKH60_05105"
     CDS             2031..>2438
                     /locus_tag="PKH60_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17449.1"
                     /translation="MSNKRDQHSLRRSRLLSLLPSQSAVLLSRATDIDYFIGFSEFLE
                     PTEREALAYFDNFKTILFIPPLTVLPKIPTITIVGDSSETTLLSHIKNPNVQSRVETL
                     FVDMRSLLAIEYLALINSYGKPIQALDRNFVWKL"
BASE COUNT          743 a          547 c          459 g          689 t
ORIGIN      
        1 agctgtagga gtaagaacca caacttcggt ttcctcgggc agttttagac tagtgatctc
       61 taattttgtt accggttcaa cctctactcc atacgtgtgc aaaaattctt caacagtttt
      121 tagctctcca aaaatagatg gctcatgttt agctgttttg taatgcatag gaataacaat
      181 agatggttca agttgccgta tcgttttaac tgccgtcgct ggatctaagg taaaaactcc
      241 ccccactgga caaaataaca catcaacact cccaattgct tcaacttgtt cggttgataa
      301 cggatgacct agatcaccca aatgacataa tcgaataccc tcaagaaaaa tggtaaaaac
      361 agtattattt cccctaagtg caccagctat gtcatcatgg aatgttggta caccgaaaac
      421 cgagatacct cccacttcgt attctccagg ttgagtgaca aggaaaggtc gctctctccg
      481 actggttggt tttagtaacc ctacagcatt gtgatcagca tgttgatgag acaccgtaat
      541 aatatctgta gtggttgatg gaaatgagaa gccaacagaa ctttgagaga atggatcggt
      601 taccacactc ccattttttc ccttcagttt gaacgatgaa tgaccgagat aaacaatttg
      661 catagtcccc cttgtgccct tatgttacca tagcactgta caaacgtaag tcttacttta
      721 acggtatact aaagtttatg cataaagaaa ccattattgc ggtggttatc gggttgcttt
      781 ttggcctctt ggttacggtt ggcgtgtaca aactgcagtc cgtaaatgac tcgagtgtgg
      841 ccacaccccc tcttgctgaa aatatttccc cctcagcttc tccttctcca gatgacttgg
      901 ccagattatc aattagttat ccaacccaag gacttatttc tgcagatgcc gatataacca
      961 ttactggcaa atctgaccca aatgcattgt tggtactatt tgccaatgat acacaacaaa
     1021 tttccgaggc ggacgacaaa ggcgcattta gcttagctct aacattaaaa gaaggaccca
     1081 attttttcac catagtagct gctaatcagg atggggtgac tttctctgca gaccgagtag
     1141 ttgtatacga aaaagaagtg ccaattgaag tttctccaga ggcaagtcaa agtgccagat
     1201 aacatctggg aaagttagat aaaccatgaa caataaaacc atttcaactc gccagctctt
     1261 cattacaatt tgcttcttag caattttttc ttgctcagtt agtcaatctg taacttccat
     1321 ttctgcccaa acgagtacaa aaagcgggtc aattgatgtc cggttggagg gtaaagacat
     1381 tggagatggt ggtagcgcta ccccaaaccc tgagagtatt aataaagcca ttaatgaacg
     1441 aattaataag caacttagtc aagaacaaga gaaaaaatta actaatataa ttgaaagtat
     1501 taatcaactt cgaagagcca tttacggcaa agttgagcga gtcacagatg attcgcttac
     1561 gattactaca gctgatggaa ttaacagtat tccactgcct gaatccgttc gggtcaaaca
     1621 aggcacaaag acaattaaaa cctcagacat cgcagttgac aattgggtac ttgttatcgg
     1681 cgccaacagc gttgatcgct caaaagaaac atccaattcc aactttacac ctcaacaaat
     1741 taccgttttt actaccaatc caattgccgc gcaaccgatg gttgagcttg gcatcattga
     1801 cagtataaca agtagttcca ttactgtgat aagtcggtct tctggcgtga gcaatacttt
     1861 ttcggttctg aagaccacca agtttcaaaa tgctaatggc gcccaaacaa ctatcgataa
     1921 ttttgaaaag gatttaaccg ttttagttgt gggttttaaa ggagaaaaat caaattctgc
     1981 aacaatcatt cgctcactcg ccacactcga taaacgcgag gaaaactaag atgagcaaca
     2041 agcgagatca gcacagtttg cgcagatctc gccttctctc acttctccca tcacaaagcg
     2101 cagtcctcct cagtagggct actgacattg attactttat cggtttttca gaatttttag
     2161 aacccactga gcgcgaagct ttagcttatt ttgataactt taaaacaata ctttttattc
     2221 ctccactcac cgtgttgccc aaaatcccaa ctattactat tgttggggac agctcagaaa
     2281 caacgctctt atcccacatt aaaaacccga atgttcaatc gagagtggag actctcttcg
     2341 tggacatgcg ctcacttctc gcaatcgagt atcttgcttt gattaattcg tatggtaaac
     2401 caatccaggc actagatcgt aactttgttt ggaaatta
//
LOCUS       DAOQZP010000099         2405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_49264_length_2405_cov_5.837447, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000099 DAOQZP010000000
VERSION     DAOQZP010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49264_length_2405_cov_5.837447"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..389
                     /locus_tag="PKH60_05110"
     CDS             87..389
                     /locus_tag="PKH60_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17450.1"
                     /translation="MKVEYDESKVSSSELPFKHVAIGEAPSFDVVDFGSILQIEKGVN
                     YIIPQEVRHCVFFLPQENCYGWLIEALLLRLTHLSSGLYRFLEKIEKLNLSIETIQ"
     gene            395..1117
                     /locus_tag="PKH60_05115"
     CDS             395..1117
                     /locus_tag="PKH60_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17451.1"
                     /translation="MSDALFPQLLPSPQRPSWAGAYVTNDGYVVYRQDYLNAAFGQIN
                     PEGTYTFGGETFSIELFLRELMHEMDHSREVKLLFDNDANLCEWFYSHSLWWFEGQRE
                     YISGIELSPLTDGEFANKLKKHLSQANFSCTQLTNTFWDLDQSPTTRNFGYQYSARWV
                     RELAERLLKLQTEKNIKGCWEKGNAIDFVEMITYEMIQARKNGTFSGDVLQFLSTFFD
                     TTIDEILEWEREWQASMVDHKI"
     gene            1130..1261
                     /locus_tag="PKH60_05120"
     CDS             1130..1261
                     /locus_tag="PKH60_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17452.1"
                     /translation="MNKDGQLSAKDLSENDLDKPGIWYRFSGWLVVNVVLRLFKYKE"
     gene            1270..2163
                     /locus_tag="PKH60_05125"
     CDS             1270..2163
                     /locus_tag="PKH60_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17453.1"
                     /translation="MSSQERLSPKQELIANFEQRSRDNFIFRLFWMYSQLYGSKRALE
                     SLPETGSLSEYCQAILNGFNLEISSELPEWLQSENGQGVLIYGPHTYHLEPFILANLL
                     SHREIYFVALSHSLFMLPDEFQAMSLPVTPSHLAKDARKMPGLAGIRQRISRSMFAKD
                     DMRSVAEMKRANLDVLQKAAELLKAGKVVIIFPAASDDIYNSGWNNGLGNIIHYLDPE
                     IDNVLLQPFDITQITFGKAIRNMRSTYVLHRPGEPTKIDVTWKPPMTLAELFATTSQI
                     AKPEPNEVVQFLAQNYQTQSR"
     gene            2213..>2405
                     /locus_tag="PKH60_05130"
     CDS             2213..>2405
                     /locus_tag="PKH60_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17454.1"
                     /translation="MKQLLRSTTHRYYLFSLLKDCAFFTAVLVPFFTEWGGISLFQVQ
                     ILQSWFMFWLFVLEVPTGAI"
BASE COUNT          718 a          481 c          480 g          726 t
ORIGIN      
        1 ttttattagt aaaagattca gatagtatac aggtatttat ccccgtttca tttggaaaaa
       61 atgcagacta ggcttataat atatttatga aagttgaata tgatgaatcc aaagtatcaa
      121 gcagcgagct acctttcaaa catgttgcaa ttggtgaagc cccttctttc gatgtcgtgg
      181 attttggttc cattcttcaa attgaaaagg gtgtaaacta cataatcccc caagaagtca
      241 gacattgcgt tttcttcctc cctcaggaga attgttacgg ttggttaatc gaagctctct
      301 tactacgttt gacacactta tccagtggtc tatacagatt cttggaaaaa atcgaaaagc
      361 tcaatctttc gatcgagact attcaatagt aatcgtgagc gatgctcttt tccctcaatt
      421 attaccaagt ccacaaaggc ccagttgggc aggcgcatat gtcactaacg atggatatgt
      481 tgtgtatcgt caagactacc tcaatgcagc atttggtcaa atcaaccctg agggaacata
      541 tacttttgga ggagaaacct tttctatcga actattcctg cgtgaactca tgcatgaaat
      601 ggatcatagt cgcgaggtaa aactactgtt tgataacgat gcaaatttgt gtgagtggtt
      661 ctattctcac tctctctggt ggtttgaggg tcaacgtgaa tatatttcag gcattgaact
      721 atcaccccta accgatgggg aatttgctaa caaactcaaa aaacatctaa gtcaagctaa
      781 tttttcttgc acacaattga caaatacctt ttgggactta gatcagtctc ccaccactcg
      841 caattttggc tatcaatatt ctgctcggtg ggtcagagag ttagctgagc gcttactaaa
      901 acttcaaact gaaaagaata tcaagggatg ttgggaaaaa ggcaacgcca tcgactttgt
      961 ggaaatgatc acctatgaaa tgattcaagc acgaaaaaat gggacatttt ctggagacgt
     1021 tcttcagttt ctcagtacat ttttcgacac aacaatagat gaaatactag agtgggaacg
     1081 tgagtggcag gcctctatgg tagatcataa gatataataa gcagtcacta tgaacaaaga
     1141 tgggcaactt tccgctaagg atttgtctga aaatgatctt gataaaccag gaatttggta
     1201 tcgtttctct ggttggctcg tggtaaatgt tgtacttcga ttgtttaaat acaaagaata
     1261 aatcccccaa tgtcttcaca ggaacgtctc tcaccaaaac aagagctcat tgcaaatttt
     1321 gagcagcgct caagagataa ctttatcttt cgtctctttt ggatgtactc tcagttgtat
     1381 ggtagtaaac gggcacttga atcactgcca gaaacgggct cactcagtga gtattgtcag
     1441 gctattctca atgggtttaa ccttgaaata agttctgagc tacccgagtg gttacaatca
     1501 gaaaatggcc aaggggtctt aatttatggg ccacatacct atcacctcga gcctttcatt
     1561 ttggctaatt tactcagtca tcgagaaata tattttgtgg cattaagtca ctctttgttt
     1621 atgcttccag atgaatttca agcaatgtcg ctacctgtta cccccagtca tcttgcaaaa
     1681 gatgctcgca aaatgcctgg attagcaggc atacgccaaa ggattagtcg ttcgatgttt
     1741 gctaaagacg atatgcgttc agttgcagaa atgaaacgag caaacttgga tgtcttacaa
     1801 aaagcagcag agctcttgaa agcaggtaaa gttgtaatta ttttccccgc cgcatctgat
     1861 gatatctaca attctggttg gaacaacggg cttggcaata tcattcacta tcttgatcca
     1921 gaaatagaca atgttttact ccaaccgttt gacattactc aaattacatt tggaaaagcc
     1981 attagaaata tgagaagcac ctatgtgctg catagacctg gggaaccaac aaaaatagac
     2041 gtgacgtgga aaccaccaat gactctagct gagctatttg ctaccacttc acaaatagcg
     2101 aaacctgagc caaatgaagt tgttcaattc ttagcacaaa attaccaaac tcagtctcgg
     2161 taaacagctt cgaaaattta tttgtaccta cctctgctaa gatagtggta ctgtgaaaca
     2221 gttgcttaga tctacgactc atcgatatta tttattcagt ctcctgaaag attgcgcgtt
     2281 ttttactgct gtgctagtac ctttttttac cgagtggggt ggtatttcac tgtttcaagt
     2341 ccaaatttta cagtcttggt tcatgttttg gttgtttgtg ctagaagtac caaccggggc
     2401 catcg
//
LOCUS       DAOQZP010000100         2339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_51431_length_2339_cov_6.490806, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000100 DAOQZP010000000
VERSION     DAOQZP010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51431_length_2339_cov_6.490806"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            617..1987
                     /gene="ltrA"
                     /locus_tag="PKH60_05135"
     CDS             617..1987
                     /gene="ltrA"
                     /locus_tag="PKH60_05135"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HNQ17455.1"
                     /translation="MKSKNPENKTNLKEESKVEDSIAFCEGNNDFTTNQRSERPNFKL
                     ELMEEVLDQSNLNLAFKRVCKNKGSPGVDGMSVDELSDYLKKNWQTIRNQLLNGNYKP
                     MPVKRVEIPKPDGGVRQLGIPTVLDRFIQQALAQVLQNGYDKTFSDSSFGFRPNRSAH
                     QAILKAQEYQNEGYKFVVDIDLEKFFDRVNHDKLMGKLRKDIQDKRVLKLIRTYLNAG
                     VMEDGVVRTSEEGTPQGGPLSPLLSNIVLDDFDSELEKRGHKFVRYADDCNIYVKTPR
                     AGERVMKSVKEFIEKKLKLKVNEKKSKVDKPAKRKFLGYSFFEKNGEAMRRIARESIE
                     RFKERVRGITRPTRRIKFIDLIRELKLYLTGWKNYFKYSDSKTTLRGLDEWVRHRIRS
                     FIWNQWAKIKTRYRMLTKFGVGHDLALQTAASRKGSWRLSNSPAVKIAFPIKYFDKFG
                     LPKLYS"
     gene            2095..>2339
                     /locus_tag="PKH60_05140"
     CDS             2095..>2339
                     /locus_tag="PKH60_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17456.1"
                     /translation="MKALIISLAALLTFSTHAATIDQFCNQFFFQDVKVRCYKIASGK
                     EFQPTAIDVCNSFFQDEEKLQCLTKIANKEYTNGELQA"
BASE COUNT          807 a          364 c          550 g          618 t
ORIGIN      
        1 gatttgttag atgatttggc cgaagtcgtt ctttctcgag gtgggcgagt gctttcttta
       61 aaacaaatcg agatgccgac aaaaagcccg ttgtttgcaa ttctttaata aaaaaaaggg
      121 agccaataag gtgatctgta ccctttaaaa agaacagttt aaaaaacaag attcatgtta
      181 gcccctgctt cggcaggggt ttttttatgt gtgccgagca tggcaaataa ttaggatggt
      241 gcaagtccat ctaccaacct gatagaggtg aaggttagtg aaacgcaagg gcgctactgt
      301 gaggtagggt ctgaaggaag cgtgtagcaa aagcacgagc tgacgtacag aaactagatt
      361 tgaggcgggg ttggaaggac gagttagcat atgataacaa agtccatatc tatcaaaatc
      421 caataacgta aactctagca gtggtgtgct gaaagttatt tgtcttacct cgggagatct
      481 catttgctgt tgaagcgctc aacaggatag ctcgtgagag ctattatcgc aaatgagaag
      541 tcagcagcgg acgtagtaag aaataaaatt ctgaaggtcc aaacaatcaa cagtggttat
      601 taggagttgg tctcttgtga aatcgaagaa tccagaaaac aaaacaaacc ttaaagaaga
      661 aagtaaagtt gaagactcga tagctttttg cgaggggaac aatgacttca caacgaacca
      721 aagatctgaa cgcccgaatt ttaaactgga actaatggag gaagttttag atcaaagtaa
      781 tttaaactta gcgtttaaac gtgtatgtaa gaataaaggc agtcccggtg tagatgggat
      841 gagtgttgat gaattatctg actacttaaa gaagaattgg caaacgattc gaaaccaact
      901 tctcaatgga aactataaac caatgccagt gaagcgagtt gaaataccta aaccagacgg
      961 tggggttcga caattgggaa ttccaacggt gttggatagg tttattcaac aagcgctcgc
     1021 acaagttctt caaaatggtt atgacaaaac tttttcagat tccagtttcg gatttcgtcc
     1081 gaacagatca gctcatcaag caattttaaa agcgcaagag tatcaaaacg aaggttataa
     1141 atttgtggta gatattgatc ttgaaaagtt cttcgatagg gtcaaccacg acaagctcat
     1201 gggaaaactt cgaaaagata ttcaagataa acgtgtattg aagctgataa gaacatatct
     1261 aaatgcggga gtaatggagg atggagttgt tagaacatca gaagaaggaa cacctcaagg
     1321 tggaccacta tcgccacttc tttccaatat tgtgctggat gattttgata gtgaacttga
     1381 aaaacgcgga cataaatttg taagatacgc ggatgattgc aacatctacg ttaaaacacc
     1441 acgtgcaggt gaacgagtta tgaaaagtgt taaggagttc attgagaaaa agttaaaact
     1501 caaagtgaat gaaaagaaga gcaaggtgga taagccagca aagaggaagt ttttagggta
     1561 tagcttcttt gaaaagaacg gggaggcgat gagaaggatt gcgagagagt cgattgaacg
     1621 atttaaggaa agggttcgtg gtataactcg cccgacaaga aggattaaat tcatcgattt
     1681 aattagggag ttgaagctat atctaacagg atggaagaac tactttaagt acagtgattc
     1741 aaaaacaact ctgcgaggac tagacgagtg ggtaaggcac agaatccgaa gctttatctg
     1801 gaatcaatgg gcaaaaatta aaacaagata taggatgctg acaaagtttg gagtaggaca
     1861 tgatttagca cttcaaacgg cggcaagtag aaaagggtct tggagattga gtaatagtcc
     1921 agcggtgaag atagcctttc caattaaata ctttgataaa tttggacttc caaaattata
     1981 cagttagaat tttaagttaa ttgaaccgcc ttggtacggt tccgtatgcc gggtggtgtg
     2041 agagggaaag gggtgtgaac cccttaccta tctctatttt atggaggaat cttaatgaaa
     2101 gctttaatta tttccttagc tgcattactt actttttcaa ctcatgcagc aactatcgat
     2161 caattttgta atcaattctt ttttcaagat gtaaaagtga gatgttataa aattgcttct
     2221 ggaaaagaat ttcaaccgac agcaatcgat gtttgtaatt cattttttca agatgaagaa
     2281 aaattacagt gtttaacgaa aattgcgaat aaagaatata ccaatggaga gctacaggc
//
LOCUS       DAOQZP010000101         2337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_51517_length_2337_cov_3.030237, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000101 DAOQZP010000000
VERSION     DAOQZP010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51517_length_2337_cov_3.030237"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..345)
                     /gene="rpsS"
                     /locus_tag="PKH60_05145"
     CDS             complement(61..345)
                     /gene="rpsS"
                     /locus_tag="PKH60_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNQ17457.1"
                     /translation="MSRSSTKGPFVDPKVLAKVSKQKLSGSKTPIKTWSRACVVSPDF
                     VGHTFLIHQGKEFVEVFITEAMVGHKLGEFAATRKFRGHGRVVKRVLSKT"
     gene            complement(362..1198)
                     /gene="rplB"
                     /locus_tag="PKH60_05150"
     CDS             complement(362..1198)
                     /gene="rplB"
                     /locus_tag="PKH60_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNQ17458.1"
                     /translation="MLRQRKATSSGVRHQVVIDRSELHKGAPEKSLLKPGHTKRQGRG
                     FKGHVTVRHRGGGVKKRIRIIDWKRVAKDIPGIVQRLEYDPVRSAHLALLFFQDGRKA
                     YVVAPQGIAVGDSIMNGEAVEIKLGNSLPLRAIPIGMAIHNIELRPGKGAQLVRGAGT
                     SATIQSKDGMYVTIQLPSKELRLVHQDCYATIGQIGNQEWKNRKIGKAGRKRLMGIRP
                     TVRGTAQHPDSHPHGGGEGRSGVGMKYPKTPWGKPAMGKKTRTKKHRTNRMIVRDRRV
                     KV"
     gene            complement(1202..1501)
                     /gene="rplW"
                     /locus_tag="PKH60_05155"
     CDS             complement(1202..1501)
                     /gene="rplW"
                     /locus_tag="PKH60_05155"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNQ17459.1"
                     /translation="MHTHILKRPIVTERSLALANKDNTYTFEVSTTASKNQVKTLIEE
                     LYGVTVTRVHTIVSQPQQRKTGRRRLGRMTARSKKALVTLKQGQTLEIFDVGGQE"
     gene            complement(1505..2305)
                     /gene="rplD"
                     /locus_tag="PKH60_05160"
     CDS             complement(1505..2305)
                     /gene="rplD"
                     /locus_tag="PKH60_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNQ17460.1"
                     /translation="MNITKITPTGKEATCVVSDLLFAAKVNQPLISQAIRVYLANQRQ
                     GTSAVQSRSDVNRTKAKIYRQKGTGKARHGSRNAPIFVGGGVAHGPSGVQNWSLLLSK
                     KQKQAAMISALSLQSKQTLVSDIPLETSGKTKYAARFLKTINPDLRKCLLILHKDTEP
                     TRRSFTNIPCVTIRSVAKLNVLDVMSADLLVFTSQAISDLETRLLANESSEKPKKTTV
                     IETKTLTAVKKSVVKKAEKPLVKKTAKKTEVKKVEAKKATKITKKTTK"
BASE COUNT          562 a          532 c          448 g          795 t
ORIGIN      
        1 acttttcttg gtgattgacg aactgatttt tgaacaactg taatctgcat ataatctttt
       61 ttatgtcttc gagagtaccc gtttgactac tcttccatga cctctgaatt ttctagtggc
      121 ggcaaactca cccaatttat ggccgaccat tgcttctgta ataaacactt caacaaattc
      181 tttaccctga tggattaaaa aagtgtgacc aacaaaatcc ggactaacaa cacaagctcg
      241 actccaagtc ttaattgggg ttttagatcc tgatagcttc tgtttactga cctttgccaa
      301 cacttttggg tcaacgaatg gtcctttagt acttgatcga gacatactct gatactcctt
      361 tttatacctt caccctgcga tcgcgaacga tcatgcgatt agtacgatgt tttttggtgc
      421 gagtcttttt acccattgct ggtttgcccc atggcgtttt tggatatttc attccaacgc
      481 cagatcggcc ttctccacca ccatgtggat gtgaatctgg atgctgggcg gtaccacgaa
      541 cagtaggcct aattcccatc agtcgttttc ggccagcctt accaattttt cggtttttcc
      601 attcttggtt gccaatttgg ccaattgttg cataacagtc ttggtgtact aaacgtagtt
      661 ctttggaagg gagttgaata gttacataca taccatcctt ggattgaatg gtggcactgg
      721 tacctgctcc tcgcactaac tgagctccct ttcctggtcg taattcaata ttatgaatag
      781 ccataccaat tggaatagca cgcagtggta atgaattacc taacttaatt tcaactgcct
      841 caccattcat gattgaatca ccaaccgcta tcccttgcgg tgcaacgaca taggctttgc
      901 gtccatcctg gaaaaataat aatgccaaat gtgcacttcg caccggatca tattcaagtc
      961 gttgcactat tccaggaatg tctttggcaa ctcgcttcca gtcaataatt ctgattctct
     1021 ttttcacacc accgccacga tgtcgaacgg tcacatgacc tttaaaccct ctaccctgac
     1081 gttttgtgtg tcctggcttc aacaaagatt tttctggggc acccttatgc agttcgctcc
     1141 gatcaattac gacctgatga cgaacaccgc tggaggttgc ttttctttga cgtaacatac
     1201 gttattcctg tccacctaca tcaaaaattt ctaatgtttg accctgcttc aacgtcacta
     1261 aagctttttt tgatctagct gtcatccgac ccaatcttcg tcttccagtc ttacgttgtt
     1321 gaggctgact cactatggta tggactcgcg tcacagtaac tccatagagc tcctcgatca
     1381 aagtcttgac ctggttttta ctcgctgtag tacttacctc aaaagtgtat gtattatctt
     1441 tattggctaa tgccagcgag cgctcagtca caattggtcg ttttaaaata tgtgtgtgca
     1501 tagattactt agtcgttttt tttgtgattt tcgtagcttt tttcgcttcg acttttttta
     1561 cctcagtctt cttggcggtt tttttcacta acggtttctc agctttttta actacactct
     1621 ttttcactgc cgtgagtgtt ttagtttcaa taactgttgt tttttttggt ttttcagagc
     1681 tttcattagc taaaagtcga gtttctagat ctgaaattgc ttgagaggtg aaaaccaaca
     1741 gatcggcaga catgacatca agtacattta attttgctac cgatcggata gtaacgcatg
     1801 gaatattggt aaatgagcga cgagttggct ccgtatcttt gtgcaagata agtaagcact
     1861 tgcgtaaatc aggattaatt gtttttaaaa atcgagctgc atactttgtt tttccagatg
     1921 tttccaaggg aatatcactc acgagcgttt gtttgctctg aagtgacaag gcggaaatca
     1981 ttgccgcctg tttttgcttt ttggataata gtaaactcca attctgtaca cctgaaggac
     2041 catgtgctac accaccgccc acaaaaatgg gcgcatttct tgatccatgg cgagcttttc
     2101 ctgtcccttt ttgtcgataa atctttgctt tagttctatt aacatcagac cgactctgaa
     2161 ctgcgctcgt cccttggcgc tgattagcca aatatactcg aattgcttga gaaattagag
     2221 gttgattaac ttttgcggca aacagaagat cactgacaac gcaggttgct tctttgccag
     2281 tgggagtgat ttttgttatg ttcatgctgt tggtttttca gttacctctg cttcagt
//
LOCUS       DAOQZP010000102         2321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_52070_length_2321_cov_6.101942, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000102 DAOQZP010000000
VERSION     DAOQZP010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52070_length_2321_cov_6.101942"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PKH60_05165"
     CDS             complement(<1..313)
                     /locus_tag="PKH60_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNQ17461.1"
                     /translation="MRLFNTMSRSTEDFSPINDKKVGMYACGFTVYDYTHLGHLRKYS
                     MDDVLVRMLRHEGYDVTFVQNVTDVGHLASDADTGEDKLEKGAKKYGQSVWDVAKKFE
                     DY"
     gene            complement(893..1192)
                     /locus_tag="PKH60_05170"
     CDS             complement(893..1192)
                     /locus_tag="PKH60_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17462.1"
                     /translation="MNVEATELAVERAVPQLCNDFSLVFQEAQYQLGQLSQLNDQELQ
                     VCIDSDSILIGLTVEEVRCIFKQRREFFDWVLKMSFEYDREYTNPIDPKTVTKNH"
     gene            complement(1266..>2321)
                     /locus_tag="PKH60_05175"
     CDS             complement(1266..>2321)
                     /locus_tag="PKH60_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ17463.1"
                     /translation="LYSHMTFKYNFDIQENQVHWTAADTGWITGQSYMVYGPLSNGIT
                     SILYEGGPAFPNPGRYWELVDRYLVQSFYTAPTVIRMLMREGEEHLNKHKLKSLRVLG
                     SVGEPINPAAWQWYSDHIGKGKAQVIDTWWQTETGGHMLVTPPSMKQKPGSAGLPFLG
                     IQPSIVDDDGNEVTQPNVVGHLVIKHPWPGALLTCWNNDDRFVQYWSEFGNKQYFYTG
                     DVAQKDSDQYYMVLGRADDVINVGGVRISTAEVESALVSHESVAEAAAIGISDLVKGE
                     AIKAFVVLNQGVVWSTELKQELKKWVRKKIGYIAEPADIEQVDTLPKTRSGKIMRRIL
                     KAKEMGLQVGDTSTMEE"
BASE COUNT          648 a          576 c          384 g          713 t
ORIGIN      
        1 aataatcctc aaattttttt gcaacatccc aaactgactg accatacttc ttagcacctt
       61 tttctaactt gtcctcaccc gtatctgcgt cggaagctaa gtgacctacg tctgtaacat
      121 tttgcacaaa tgtgacgtca tacccctcat gtctaagcat tcgcaccaaa acatcatcca
      181 tactgtattt tcttaaatgc cccaagtgag tgtagtcata caccgtaaaa ccacaggcat
      241 acatacctac ttttttatca ttaattggtg aaaaatcttc agtagaacga ctcatcgtat
      301 taaataatcg catgcaccta ttgtaaacca cttttagaat tcagagaaaa ctcatttgcc
      361 caaccacatc ctcaccaaac tatttctacc atagtagtta aagtagtttt acaaaagtaa
      421 agttcatcta caaaagtaaa gttgctctat gcttccaact tcgtgtgtct gcatcacgtg
      481 ttctgtccgt aaatctgaga gcgttttcag cgcaattctc agatcacgct ccgattgggg
      541 caatactact ctccgatcga tcagttcgaa tgcaaacaaa atatctagtt cagcttgggt
      601 taatttttta ggatcagttt agattaaaat catcttaacg cacgtcaaaa cgcttaatta
      661 atagattttg ttgctccgat cgaatctctt ttggagttcg tattgcaatc cgtagctctc
      721 gttgctcctc ggagagtatt actcgtctat caatgaaccc aaataaatat aggaggtcat
      781 attctttctg aagaagaaag gctggatgca tttcatgcca cacccctgaa ctcaatggtc
      841 taaatgtttt tccgctagct cctcgcacag tcggaaacct agaatctagt agttagtggt
      901 ttttcgtcac tgttttagga tcaataggat ttgtgtattc cctatcatat tcaaaactca
      961 tcttgagaac ccaatcgaaa aactctcgtc tttgcttaaa aatgcatctc acttcttcca
     1021 cagttaaacc tatcaggatc gaatcagaat cgatacatac ctgcaattct tgatcattca
     1081 gctgtgacaa ttgacccaac tgatactggg cttcttgaaa tactaatgaa aaatcattac
     1141 atagttgcgg aactgctcgc tcaacagcca attcagttgc ctcaacattc atagctctcc
     1201 aaataactat ttactaaact ttagtatatc aatataattt aaaatagatc taaatatcac
     1261 gctgattact cctccatcgt cgaagtatca ccaacctgca aacccatctc cttcgctttc
     1321 aaaatcctcc gcataatttt tccactacgt gtttttggta atgtatctac ttgctcaatg
     1381 tctgctggct ctgcgatgta cccaattttc ttgcgcaccc atttctttaa ttcttgtttt
     1441 agttctgtgc tccaaaccac accttggttg agcactacaa acgctttgat tgcctcccct
     1501 tttactagat cgctgatgcc aatcgcggct gcctccgcga cactctcatg tgataccagc
     1561 gcactttcca cttctgcagt gcttattctg actccgccta cattaatcac atcatcagct
     1621 cttcccaaca ccatgtagta ttggtcacta tctttttggg caacatctcc agtatagaaa
     1681 tactgtttat tgccaaactc actccaatac tgaacaaagc gatcgtcatt attccagcag
     1741 gtaagcaatg cgcctggcca agggtgtttg atgaccaaat gaccaactac atttggttgt
     1801 gtaacctcgt tgccatcgtc atccacaatg cttggctgta tgcccaaaaa gggtaaaccg
     1861 gcgctacccg gcttctgctt catagaggga ggtgtcacca gcatgtgacc ccccgtttct
     1921 gtctgccacc aggtatcaat aacttgagct tttcccttac caatatgatc ggagtaccac
     1981 tgccaagcag cggggttaat tggttcgccc acactaccta gtacccgcaa cgattttagt
     2041 ttgtgtttat ttagatgctc ttcaccttcg cgcatcaaca tgcgaataac agtgggtgca
     2101 gtgtaaaaac tttgtaccag atatctgtcc actaattccc aatatcggcc tgggtttgga
     2161 aaagcaggcc caccttcata aagaattgag gtgataccat ttgatagcgg accatacacc
     2221 atgtaacttt ggccagtaat ccaaccggta tctgcggctg tccaatgaac ttgattttct
     2281 tgaatatcaa agttgtactt gaacgtcata tgagagtaca a
//
LOCUS       DAOQZP010000103         2271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_53830_length_2271_cov_4.963448, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000103 DAOQZP010000000
VERSION     DAOQZP010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53830_length_2271_cov_4.963448"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..561)
                     /locus_tag="PKH60_05180"
     CDS             complement(13..561)
                     /locus_tag="PKH60_05180"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNQ17464.1"
                     /translation="MNATNSPYEFYFIRKSPLILLSKLFLFEIIFFAVYILVRVPKLF
                     LTEVIPVDILLDLNFWSFVVFSVFSFLQVIMAVLVTLQWTNEYYIIRSSEILHRRGTF
                     TLKEETFSLNNVEAFTVEESFWGKLFHFGTVRFYSPVLKVEYSLEEVPDPLELKKTIE
                     RMVKQNRSQHQRGETIIPIRRR"
     gene            complement(564..637)
                     /locus_tag="PKH60_05185"
     tRNA            complement(564..637)
                     /locus_tag="PKH60_05185"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(601..603),aa:Ile,seq:gat)
     gene            complement(661..>2271)
                     /locus_tag="PKH60_05190"
     CDS             complement(661..>2271)
                     /locus_tag="PKH60_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNQ17465.1"
                     /translation="YIEKDAALERRFQPVMVEEPSVEDTISILRGIKEKYEAHHGVRI
                     RDNALVAAAELSDRYINDRFLPDKAIDLIDEAASGIRIAIDSKPIELDSMERKIRQLE
                     IEKEALKKEKDDASRARLNEIEKSLAELREDYQGLETHWQNEKKIIDQIKENSKQIDQ
                     LREEATQAERDLNLQRVAEINYGKIPELQKQLDQLQKDLVKLQKDRKILREEVSEEDI
                     AAVLSHWTGIPAQKMLTAESDKLAHMEKHIGQRVISQTAAIQAVSRAVRRNRAGISDE
                     NRPIGSFLFLGPTGVGKTELAKALAEFLFNNERAIVRIDMSEYMEKHAVARLIGSPPG
                     YVGYEEGGQLTEAVRRHPYTVILFDEIEKAHPEVFNVLLQVLDDGRLTDSKGRTVDFK
                     NTVIIMTSNLASQEIAELQEEPEKQKQAVENQLHQMFRPEFLNRLDDVIIFQPLTPED
                     IAEIVKIQLQKVTERLAKKQITVQFQPDAIALLASKGFDPVFGARPLKRIIQNEILDE
                     IALKLIDGTIREGQKLSVSVDTGHFVIK"
BASE COUNT          591 a          496 c          438 g          746 t
ORIGIN      
        1 acttcaattt gattagcgac ggcggatggg aataattgtt tctcccctct gatgctgaga
       61 tcgattttgt tttaccatcc gctcgatagt tttcttcaat tctagcgggt caggtacctc
      121 ttcaagtgaa tattctactt ttaggactgg actatagaat ctaaccgttc caaagtgaaa
      181 taatttaccc caaaatgatt cctctactgt aaaggcctca acattgttga gtgaaaaagt
      241 ctcttctttg agagtaaatg tacctcttcg atgcagaatt tccgaagagc ggataatgta
      301 atattcattc gtccactgta gtgttactag cacagccatg atgacttgaa ggaagctaaa
      361 cacagaaaac acaacaaaag accaaaaatt tagatccagc agaatatcga ctggaatcac
      421 ttccgttaaa aataactttg gaacgcgcac gaggatgtat actgcgaaaa aaataatttc
      481 aaacaaaaat agctttgaca gcaaaatcag gggcgatttt ctgatgaagt aaaattcata
      541 cggactatta gttgcgttca tagtgggtga gcttggactc gaaccaagga cctctatctt
      601 atcaggatag cgctctaacc agctgagcta cacacccacc gttctacctt gagaacccga
      661 ctattttatc acaaaatgac cagtatcgac tgagacgctg agtttctgac cctccctgat
      721 tgtaccgtca attagtttga gcgcaatttc gtctaatatc tcattttgaa taattcgctt
      781 gagtggtctg gcaccaaaca cggggtcaaa ccccttacta gcgagtagtg caatggcatc
      841 gggctgaaat tgtacagtta tttgtttttt tgctaagcgc tcggtaactt tttgaagttg
      901 gattttgaca atttcagcaa tgtcttctgg tgttagaggt tgaaagataa tcacatcatc
      961 tagccggttg agaaattctg gcctaaacat ttggtgcaat tgattttcaa ctgcctgctt
     1021 ttgtttttct ggttcttctt gcagttcggc aatttcttga cttgccaaat ttgatgtcat
     1081 gatgatgacg gtatttttaa aatctaccgt tctccccttg ctatccgtga gtcgaccatc
     1141 atcgagaacc tggagtaaaa cattgaaaac ttctggatgt gctttttcaa tttcatcaaa
     1201 aagaataacg gtgtatgggt ggcgtctaac tgcctcagtc agttgaccgc cttcttcgta
     1261 accaacgtag ccagggggcg agccgatcag gcgagccacg gcatgctttt ccatgtattc
     1321 gctcatgtca attctcacaa tggctcgttc attgttaaac aaaaattcag cgagagcctt
     1381 ggctagttct gttttcccta ctccagttgg tcctagaaac aaaaatgatc caattggtcg
     1441 attttcatcg ctaattccag ccctatttct acgaactgct ctagatactg cttgaattgc
     1501 agcagtttga ctaatcacac gttgaccaat atgtttttcc atgtgggcca atttatcact
     1561 ttctgctgtc agcatttttt gcgcaggaat accagtccag tgtgataata cagccgcgat
     1621 atcttcctca cttacctctt cgcggagaat ttttcgatct ttttgtaact taaccaagtc
     1681 tttttgcaat tgatcgagct gtttctgcaa ctctggaatt ttgccataat tgatttctgc
     1741 cactcgttga agattcaaat ctcgttctgc ttgcgttgct tcctcgcgta attgatcaat
     1801 ctgcttactg ttttctttga tttgatcaat aatttttttc tcattttgcc aatgagtttc
     1861 tagcccctga tagtcttccc gaagctcagc taaggatttt tcaatttcgt ttaacctggc
     1921 tcgtgaagca tcatctttct ccttttttaa tgcctctttt tcaatttcta gttgtcgaat
     1981 ttttcgttcc attgagtcta gctctatggg tttactgtcg atggcaatac ggattcctga
     2041 agcggcttca tctattaaat caattgcttt atcaggtaaa aatctatcat taatatatcg
     2101 atcggaaagt tcggcagcgg ccaccaacgc gttgtctcga attctgacgc catgatgagc
     2161 ttcatacttt tcttttatgc cgcgaaggat tgaaatagta tcttcaactg agggttcttc
     2221 caccatcacg ggttggaagc ggcgctccag cgccgcatct ttttcaatat a
//
LOCUS       DAOQZP010000104         2237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_55131_length_2237_cov_5.637489, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000104 DAOQZP010000000
VERSION     DAOQZP010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55131_length_2237_cov_5.637489"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..100
                     /locus_tag="PKH60_05195"
     tRNA            30..100
                     /locus_tag="PKH60_05195"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:62..64,aa:Trp,seq:cca)
     gene            107..436
                     /locus_tag="PKH60_05200"
     CDS             107..436
                     /locus_tag="PKH60_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17466.1"
                     /translation="MINQDMEHMGELGDLKDTKYWFQRDLQTMKKGLNSAIGVLPYLI
                     IVALIASVIINCIEQTLFFSTVVLLAVMIFPIIFLGYFLFYIMVNKGYNIHSSGGGTV
                     IDDSRGK"
     gene            443..1252
                     /locus_tag="PKH60_05205"
     CDS             443..1252
                     /locus_tag="PKH60_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4111 domain-containing protein"
                     /protein_id="HNQ17467.1"
                     /translation="MINNDYIEIPASVKKCIDELQVHYTQLLGSDLIGIYLHGSIAMG
                     CFNEVSSDIDVMVVVEEPLTTDIKKQLGQIHLELSATYRKNIELSVVTRETLINFVYP
                     TPFEFHYSDGHKQSFLDEVVDFETAKQDYDLAAHFVILKTYGITLVGEQAEIMFPEIS
                     KKDYVDSITRDFEWSFYNIMASFDERNCRVPPYAVLNSCRVLAFLDSGLITSKQSGAE
                     WAQHQLPSKFQSLIYEALMEYKNAGSSHPVEGRLLKEFAQYVRRTILELNG"
     gene            complement(1249..1746)
                     /locus_tag="PKH60_05210"
     CDS             complement(1249..1746)
                     /locus_tag="PKH60_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNQ17468.1"
                     /translation="MEMVDIINQQNEVLYQTSKEEAHQKGLLHRCIIAEVIDSKGNWT
                     LVKQASDRQDADQYVSPVGGHIKAGESETEALAREALEEMGLTSFTSKYIGKAILNRE
                     VIGRKENHLFILYEIYTDQTPTLNHESVAYEKFTVTQLKKEIKSNPSKFGQAFHFVME
                     KFYNY"
     gene            complement(1750..1953)
                     /locus_tag="PKH60_05215"
     CDS             complement(1750..1953)
                     /locus_tag="PKH60_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNQ17469.1"
                     /translation="MSLINVIGYYLALKALEVGTAVQVVPMIQTYSIITVIFAAIFLK
                     EREHLLLKFFAAILALIGIQLVL"
     gene            complement(1996..>2237)
                     /locus_tag="PKH60_05220"
     CDS             complement(1996..>2237)
                     /locus_tag="PKH60_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17470.1"
                     /translation="HSIGAGLILISIILVSMPKSKKVAITTIGLLFGFLTSSLIGIAQ
                     IFDKAGAENFSPALYSIIVWLAPIPFVIFPRLKLS"
BASE COUNT          645 a          401 c          484 g          707 t
ORIGIN      
        1 tttcattgta cagtataatc agtcttcata gggctatgat gtaatggtag cattaaggtc
       61 tccaaaacct tccgtctagg ttcgaatcct agtagccctg caagatatga tcaatcaaga
      121 tatggaacac atgggtgaat tgggtgatct taaggatact aagtattggt ttcagcggga
      181 tttacaaact atgaaaaaag gtttgaattc tgcaattgga gtattgccgt acttaataat
      241 cgtcgcctta atcgcgtctg tgattattaa ctgtattgaa caaacactgt ttttttctac
      301 ggtagttttg ctagcagtca tgattttccc gatcattttc ttaggttact ttctcttcta
      361 tattatggta aacaaagggt acaacattca ctctagcggc ggtggcaccg tgattgatga
      421 ttctcgtgga aaataaggaa tagtgataaa taacgattat attgaaattc cagcgagtgt
      481 taaaaagtgt atcgatgagc ttcaagttca ctacacgcaa ttgctcggct cagatttaat
      541 aggaatctat ttacatggtt cgatagcaat gggttgtttt aatgaagtat cgagtgacat
      601 agatgtgatg gttgttgttg aggaacccct aactaccgac ataaaaaaac agctcggcca
      661 gatacatctt gagctctctg caacataccg aaaaaatatc gagctcagtg ttgtgactcg
      721 tgaaacactg attaattttg tatatccaac cccttttgag tttcattact ctgatggtca
      781 caaacaatca tttttggatg aagtagttga ttttgaaaca gccaagcagg attacgacct
      841 ggctgcccac tttgtgatcc taaaaacata tggcattaca ctcgttggtg agcaggcaga
      901 aataatgttc cctgagattt cgaaaaagga ctatgtggat tcgatcactc gcgactttga
      961 gtggagtttt tacaatatca tggctagctt tgatgaaagg aactgtcgtg ttcccccgta
     1021 tgctgtgtta aattcttgtc gtgtgttggc atttcttgat tctgggttga tcacgtccaa
     1081 acaatctggg gcagagtggg cacagcatca actaccttca aaatttcaaa gtcttatcta
     1141 tgaagcactt atggagtaca aaaatgctgg atcttctcac ccagttgaag gaaggttgct
     1201 gaaagaattt gctcagtatg tgcggcgtac aatacttgaa ttgaatggtt agtaattgta
     1261 gaacttttcc atcacaaaat gaaaagcctg accaaactta gacggatttg atttaatttc
     1321 tttttttagt tgagtgacgg tgaatttttc ataagccact gattcgtggt taagcgtggg
     1381 agtttggtcg gtgtagattt catagagaat aaacaagtga ttttcttttc taccaatgac
     1441 ctcgcggttt aagatagctt ttccgatata tttagaagtg aacgaggtta gtcccatttc
     1501 ttctagggct tcgcgcgcaa gcgcctcagt ttcgctttct cctgctttga tatggcctcc
     1561 aactggggaa acatattggt cagcatcttg tctgtcgctg gcttgtttga ccagcgtcca
     1621 gttgcctttt gagtctataa cttcagcgat aatacaacga tgaagtaagc ctttttgatg
     1681 ggcttcttcc tttgaagttt gatataaaac ttcgttttgt tggttgatga tatccaccat
     1741 ttccataggt tataacacta attgtatgcc aatgagcgcg aggatggctg cgaaaaattt
     1801 cagcagtaga tgctcgcgtt ctttcaaaaa tatggcggca aaaatcaccg tgatgatcga
     1861 ataggtttga atcattggta ccacctgaac tgctgttcca acctccagcg cttttaatgc
     1921 aaggtagtaa ccaataacat taatcaacga cataataaag atagatttgc ctgattgacg
     1981 aatttcttgt ttgatctatg aaagttttaa tctgggaaag ataacaaaag gaatgggggc
     2041 tagccacacg atgattgaat agagtgcggg tgaaaaattt tctgcaccag ccttgtcaaa
     2101 aatttgggca ataccaatga ggctactagt gagaaacccg aatagtaaac caattgttgt
     2161 gatagcaact ttttttgatt taggcatcga aactagaatg atagagatta agatgagtcc
     2221 cgcacctatg gagtggt
//
LOCUS       DAOQZP010000105         2232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_55337_length_2232_cov_4.377124, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000105 DAOQZP010000000
VERSION     DAOQZP010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55337_length_2232_cov_4.377124"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PKH60_05225"
     CDS             <1..570
                     /locus_tag="PKH60_05225"
                     /inference="COORDINATES: protein motif:HMM:NF019847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNQ17471.1"
                     /translation="FGQFLVQQKPDLTINYTGLDNSAPLLKYAGEKLQSVKLKPQLIE
                     LDLIEHLLSEQNLLEKQSFDVVVVFGVFHHIPSHDLRKRLLAQLSALVALNGILCIGF
                     WQFLNSVKLRERTVDPKTLDLNATDFEENDYILDWRTGEKPAYRYCHYTNNEEEIALL
                     KQSKLKVIDEFFADGATHNLNHYLVLKKS"
     gene            complement(539..2062)
                     /locus_tag="PKH60_05230"
     CDS             complement(539..2062)
                     /locus_tag="PKH60_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17472.1"
                     /translation="MLRAEIHSFNSSGFQEETKEQGAEIPDWQELIAKYEQEIANEPL
                     ETVRFAEGHMGIISSPNHFGQAVVDAELIEKMVAFESERLGLDPDLSLLSQLGEDDFV
                     LLDASLNITSIINNSTLDQGEDITKELVTLRQAFKAVVTEAAELARIHKRWVSIEQDK
                     KSKPEVIAKSIAIGVKLVREYVEQFVEEPEAIILTSSFLPNDMARLVIDQALLEGVLT
                     KKPKYYEIRLYCAGTVAALEVILSNPVFSEMSNVAILALEPISALIEDRHYSIEDNRF
                     QSAVIFSDDFLIMRVQPSEFYLRPESILRAIDDRGVIKLDSWSEFPSGENPPQRADWL
                     QRLLGENNQDILFIDETHMIIQMRSPEEDQPRAEMNPKATAKFFSFHSVLIILEILKQ
                     VEIKHVHNHEPSGGVIVITDHGVTKKISRNPELILQTPESVPKLIFLGVGNSSSGTFL
                     RQLFELAKRGLLSDQAFRETTLGYAPGAGATFAAAVIEARKTAQTEEVTIFSAPDND"
BASE COUNT          655 a          490 c          414 g          673 t
ORIGIN      
        1 tttggtcaat ttcttgtcca gcaaaagcct gatctcacca tcaactacac cggccttgat
       61 aattcagctc cgttactcaa atatgcagga gaaaagcttc aatccgtcaa actcaaacca
      121 caattaatcg aactggatct catcgagcac ttgctctcag agcaaaattt gcttgaaaag
      181 cagtcgtttg atgtagtggt agtttttggc gtattccacc acattccatc tcatgattta
      241 cgcaaaagat tacttgcaca actttcagca ttggttgccc taaatggaat tttgtgcatt
      301 gggttttggc aatttcttaa ttcagtcaaa ctacgagagc ggacagtcga tccaaaaacg
      361 ctcgatttaa acgcaactga ctttgaagaa aatgactata ttcttgactg gagaacaggg
      421 gagaagccag cctatcgcta ctgccattac accaataatg aagaagaaat tgcccttctc
      481 aaacaatcaa aactcaaagt cattgacgaa ttcttcgccg atggcgcaac tcacaatctt
      541 aatcattatc tggtgctgaa aaaatcgtaa cttcttccgt ctgagcagtt tttcttgcct
      601 ctatgactgc agcggcaaat gtagcccctg ctccaggagc atatcccagt gtagtctcac
      661 gaaatgcctg gtctgaaagt aaaccacgtt tggccagctc aaataattgt cttaaaaaag
      721 tcccagagct agaattaccc acacctaaaa agataagctt cggaactgat tcgggagttt
      781 gaagtattaa ctctggattc cgagagattt tcttcgtgac accatgatca gttataacaa
      841 tgacgccacc agatggttcg tgattatgaa cgtgcttaat ttctacctgt ttaagtatct
      901 ctagaattat taaaaccgag tgaaatgaga aaaacttcgc agttgccttt gggttcattt
      961 cagctcttgg ctggtcttct tctggacttc gcatctggat aatcatgtga gtttcatcaa
     1021 taaacaagat gtcttggttg ttctctccga gtaagcgctg caaccaatcc gctctttgag
     1081 gaggattttc accggatggg aattcagacc aagaatcgag ctttattact ccacgatcgt
     1141 cgattgctcg caatattgat tctgggcgta agtaaaactc tgaaggttgt accctcatga
     1201 tcaaaaaatc atcactaaaa ataaccgcac tttggaaacg attgtcttca atactgtagt
     1261 gacgatcttc aatcagcgcg ctgattggtt caagcgctaa gattgcaaca ttgctcattt
     1321 ctgaaaaaac agggttggac aatatgacct caagcgccgc aacggttccc gcacaataca
     1381 accttatttc atagtattta ggtttttttg ttaacactcc ctccaacaac gcttgatcaa
     1441 taactaatct tgccatgtcg ttgggcagaa atgaggaagt caaaataatg gcctctggtt
     1501 cctcaacgaa ttgttcaaca tattctctaa ccaacttgac cccaattgcg attgatttgg
     1561 caatcacctc aggttttgat tttttatctt gttcaataga cacccacctt ttatgaattc
     1621 gtgctaattc agcagcttct gttacgaccg ctttaaaggc ttggcgtaat gtcaccaact
     1681 cttttgttat gtcctcacct tgatccaagg tcgagttatt aatgatagaa gtaatattga
     1741 gagaagcatc taataaaaca aagtcatctt cgccaagctg agagagtagc gataagtctg
     1801 gatctaatcc taggcgttca gattcgaatg caaccatttt ttcaatgagc tcagcatcga
     1861 caactgcctg tccaaaatgg tttggggatg aaatgattcc catgtgccct tcagcaaatc
     1921 gaactgtttc caagggttca ttagcaattt cttgttcgta tttagcaatt aattcttgcc
     1981 aatctggaat ttcagcacct tgctctttgg tttcctcttg gaatccagat gaattaaaag
     2041 aatgtatttc agctcgcaac atccagttac ggtaatcgga tcatcaactt ttcacaatat
     2101 gcagatagta tgtaagcatc caaatcatat tagtttataa tcaactctaa ttatgaaaca
     2161 acttctatcg atggctctca tctttttggc tttactggga atcgcagatg caggctacat
     2221 tagttatgaa aa
//
LOCUS       DAOQZP010000106         2201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_56576_length_2201_cov_4.394688, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000106 DAOQZP010000000
VERSION     DAOQZP010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56576_length_2201_cov_4.394688"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PKH60_05235"
     CDS             <1..592
                     /locus_tag="PKH60_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNQ17473.1"
                     /translation="ILAVVLVASITIHEFAHAWAADHLGDPTPRSQGRITLNPLAHLD
                     PLGTLAILLIGFGWGRPVVFDPYNLKNPVKDVALIALAGPASNLILATIVAVAFKLGL
                     LGFLSIFGASAIITLIVSLNVMLAIFNLIPVFPLDGEKILVALLPRTTAIEYSQFMRR
                     YGMIVLLFLLAPWSGNSPISRLISPIIGFITNLLIG"
     gene            604..1365
                     /locus_tag="PKH60_05240"
     CDS             604..1365
                     /locus_tag="PKH60_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17474.1"
                     /translation="MSKSVEHSRMELEAMHAEFLRRVYDRDSQPELSPLTEQFQLHAM
                     REATGRQVTGNNYTVDSLHVKKIEYINQFGYFDICVWVTNPDQKIVFISPEIYSRGLS
                     GSESGHGKTTLVALHKFLQNLANQSGFTIVCFSSLNVGSEQLNAMLGFPIPQLLKTHV
                     SNFKKLVPRDQFINWLELLETLPHEASYYYQFKPAEEQAVSESHPQLPTIIDFLGLNQ
                     TNNDQEEKPVRGLGKGLKNLRAILENRFAQKNHHS"
     gene            1400..1645
                     /locus_tag="PKH60_05245"
     CDS             1400..1645
                     /locus_tag="PKH60_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17475.1"
                     /translation="MYHAVDEAIDVVGSYSHGRFVPKKFRWGKRILMIDQVTMQSDIK
                     DGGVRKRMVSVTVDNEVYRLEFNRETEQWYLREVWVE"
     gene            complement(1675..>2201)
                     /locus_tag="PKH60_05250"
     CDS             complement(1675..>2201)
                     /locus_tag="PKH60_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17476.1"
                     /translation="AEPNSYDLAVQLADRFIGELIRVDVSEAFSTQLAGAIDALLRKN
                     RPDIIQAEQAMADEEAMREFFSPAEKYGSLELKGAQDCDPLYDEIISGLDFPLKATYT
                     NTQIPYFSITLWNAEFVRILVPPFFPTSQDKRLMLVFTSSDVSTESISSFGAPVLANI
                     IKSHVTTTDTGSAQ"
BASE COUNT          636 a          490 c          431 g          644 t
ORIGIN      
        1 tattttagca gttgtgctgg tggcctcgat taccattcat gagtttgctc atgcttgggc
       61 tgccgatcat ctgggtgatc caacccctcg cagtcagggt cgtattacac ttaaccccct
      121 cgcccacctc gatccgcttg gcactttggc aattttatta attggttttg gttggggccg
      181 gccagttgtg tttgacccct ataacctgaa aaatcccgtc aaggacgttg cgttaatcgc
      241 actggccggc ccagcttcaa atctcatcct ggcaacgatc gtggctgttg ctttcaaact
      301 aggtctgtta ggcttcttaa gtatctttgg cgccagcgca attatcaccc tgatcgtttc
      361 gctcaacgtc atgctcgcca ttttcaacct tataccagta tttcctctgg atggggaaaa
      421 gatcctcgtc gctctgctcc ctcgcaccac cgccattgaa tacagtcaat tcatgcgtcg
      481 ctatggcatg attgttttac tcttcttact cgctccctgg agtggtaact cccctatctc
      541 cagactaatc tcaccgatta tcggttttat taccaaccta ctaattgggt aaccaatatt
      601 caaatgtcaa aatcggttga acattctcga atggaactag aggcgatgca tgctgaattt
      661 ctgagaagag tttatgaccg agattcccag ccagaactca gtcccttgac tgaacaattt
      721 caactgcatg cgatgcgtga agctacaggc aggcaagtta ctggcaataa ctatactgtt
      781 gattcgttac acgtaaaaaa aatcgaatac attaatcaat ttggttactt cgacatctgc
      841 gtttgggtta caaaccctga tcagaaaatt gtcttcattt caccagagat atactctaga
      901 ggactttctg gcagcgaatc aggacatggt aaaactacgc ttgttgcgtt acataaattc
      961 ctccaaaatc ttgctaatca atctggcttt acaattgtct gtttttccag tctcaatgtt
     1021 ggatctgaac aacttaacgc aatgcttgga tttcccattc cacaacttct taaaacccac
     1081 gtttcaaatt ttaagaagtt agttcccaga gatcaattta tcaactggct tgaattacta
     1141 gaaacattac cccatgaggc tagttattat taccaattca aaccagcaga agaacaagca
     1201 gtgtcagaat cgcaccctca attacccaca atcatagatt ttcttggttt aaatcaaact
     1261 aataatgacc aagaagaaaa accagtacgt ggtcttggta aaggtctgaa aaacttgaga
     1321 gcaatacttg aaaatcgctt tgcacaaaaa aaccaccact cttgactctt cgggtattgt
     1381 tttggtatga cagtggttga tgtatcacgc tgttgatgaa gcaatcgatg ttgtaggtag
     1441 ctatagccat ggcagatttg tgccaaaaaa attccgttgg ggcaaacgca tactaatgat
     1501 cgatcaagtg accatgcaat ctgatattaa agatggtggt gtgcgcaaac gcatggtaag
     1561 cgtgaccgtc gataatgagg tttatcgatt agaattcaac cgagaaactg agcagtggta
     1621 tctcagagag gtttgggtgg aataatcttg aaaagataaa attaatcaca tgacttattg
     1681 agcactacca gtatcagtcg tagtcacatg gctctttatt atattggcaa gaaccggtgc
     1741 cccaaaggaa ctaatactct cggtagagac gtcagaactc gtaaatacta acataagtct
     1801 cttatcttgg gatgtcggaa aaaaaggtgg caccaaaata cgtacaaact cagcattcca
     1861 caacgtgata gaaaaatagg gaatttgagt gttcgtatac gtagccttta aaggaaaatc
     1921 aagtccactg ataatttcat catatagtgg atcacagtct tgagctcctt ttaattcaag
     1981 tgaaccatat ttttctgcgg gactaaaaaa ttctctcatt gcctcttcat ctgccattgc
     2041 ttgctcagcc tgaataatat ctgggcgatt tttgcgtaac agcgcatcaa tagctcctgc
     2101 tagttgagta gaaaaagctt cagaaacgtc aaccctaatt aattcaccaa taaacctgtc
     2161 ggctaattga actgctaaat catacgagtt aggttctgct t
//
LOCUS       DAOQZP010000107         2142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_59072_length_2142_cov_4.449928, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000107 DAOQZP010000000
VERSION     DAOQZP010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59072_length_2142_cov_4.449928"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PKH60_05255"
     CDS             complement(<1..376)
                     /locus_tag="PKH60_05255"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HNQ17477.1"
                     /translation="MKKLIKVSTKTGDSGESGLIDGVRVPKNHPLFAAIGDVDELNSW
                     LGLIVAKLSNEFSAHKTYLLAVQDALFYLGAELARSKNTKLSPSMVDELEKRSDELQQ
                     QLADDWHTKFLLPGGTELGGYLD"
     gene            462..878
                     /locus_tag="PKH60_05260"
     CDS             462..878
                     /locus_tag="PKH60_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17478.1"
                     /translation="MNRQEEIPEPKLDPKVEKLEKTKKLFLEIITGLPDGIGQQLIAH
                     HFFYHEYRLKMYDLKPNRDYLQVDSTLRVHEGLVVEHKMKIDILVATYLHLGTREMAF
                     ELDHLCITTSRDSMYQLWNIMQEVLSNGGDSSVASF"
     gene            941..1936
                     /locus_tag="PKH60_05265"
     CDS             941..1936
                     /locus_tag="PKH60_05265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNQ17479.1"
                     /translation="MQLITTVITNSSVFFDLHQEWHYLLENSVVNEIFLTPEYQSVWW
                     QTLGRGELHIISVRSDSGTLVGLAPLFVDDGVYNFIGCKDVSDYLDFIVHKNYKELVY
                     QVLFDELKSINQASYSLCSIQKDSPTLIQLPKLFSGKEKLSTTEQAVCPQIQLPNTWD
                     SYLNNLNRKQRQEIKRKWKKIETSEDVEYQCISEEHAATQAVSLFIELHQQSSETKRD
                     FWDPHHKAFFEQLLPVLARRGWLKLFFLSIGRHTKPIAAMLIFDYGGHYDLYNSGFNP
                     DFSSLSPGQVLTAYTIKDAIENGRTLYDFLRGNEEYKFRLGGIPKPIYDLSFSIK"
BASE COUNT          714 a          412 c          392 g          624 t
ORIGIN      
        1 tatctaaata accccccagt tctgttcccc ccggtagcaa aaacttggtg tgccaatcat
       61 cggcgagttg ttgctggagt tcatctgaac gtttttcgag ctcatcaacc atcgaaggac
      121 ttaacttagt gttttttgac cgagccaact ctgctcctaa ataaaaaaga gcatcttgaa
      181 ccgccagtaa atacgttttg tgtgccgaaa attcattgct gagttttgct actattaagc
      241 ccaaccaaga atttagttca tctacatcac caatagcagc aaataaaggg tgatttttag
      301 gtacacgcac accatcaatc aagccagact ctccactatc accggttttg gtggaaactt
      361 taattaattt tttcataacc atactgtatc agacttgaca agaaggataa agaagatgaa
      421 aatatgttag atttatagta gaaaatatca aaacgagaaa tatgaataga caggaagaaa
      481 tacctgaacc gaaactagac ccaaaagtgg aaaagttaga aaagacaaaa aaactatttt
      541 tagaaataat aactggtcta cctgacggaa ttggacaaca attgattgca caccactttt
      601 tttatcatga atacagatta aaaatgtatg acctcaagcc aaatagagat tatttgcaag
      661 tcgactcaac acttcgggta catgaggggc tagtggtcga acataagatg aaaatcgata
      721 tacttgttgc tacatacttg catttaggta ctcgcgagat ggcttttgag ttagaccatc
      781 tttgcataac aacttcaaga gacagtatgt accaattgtg gaatatcatg caagaagtat
      841 tatctaacgg tggggattct agtgttgcta gcttttaatt tgaagtccaa tctcacgagc
      901 aaatgatttc ttaccataga gagctgtata ctagcaacgt atgcaactaa tcacgacggt
      961 tatcacaaat tcctctgttt tttttgatct tcatcaagaa tggcactatc tcttagagaa
     1021 ttcagtagta aatgaaattt ttttaacacc tgaatatcaa tctgtgtggt ggcaaacact
     1081 cggtagggga gaattacaca tcatcagtgt tcgttcagat tctggaacat tagttgggtt
     1141 ggcaccactg tttgttgatg atggagtgta taacttcatc ggctgtaaag atgtaagtga
     1201 ttatctagat tttattgtgc ataaaaacta taaagaattg gtttatcaag tactatttga
     1261 tgagttaaaa tcaattaatc aagcctcata ttctttatgt agcatccaaa aagattcgcc
     1321 aactttaatc cagcttccaa aattattcag tggtaaagaa aaactctcaa ccaccgagca
     1381 ggcagtctgt ccccaaattc aactgccaaa cacatgggat tcctacctaa acaaccttaa
     1441 tcggaaacaa cgtcaagaaa ttaaacgtaa gtggaaaaaa atcgaaacat ctgaagatgt
     1501 tgagtatcaa tgcatttctg aagaacatgc ggccacacag gctgtttcgc tttttattga
     1561 actacatcaa caatcaagcg agaccaaaag agatttttgg gatccacatc acaaagcgtt
     1621 ttttgaacaa ctacttccag tgctggcaag acgtgggtgg ctcaaactat tttttctgtc
     1681 aatcggtaga catacaaaac caattgccgc catgctgatt tttgattatg gcggtcatta
     1741 cgatttgtac aactccggtt ttaatcccga tttttccagt cttagtcctg gtcaagtttt
     1801 gactgcttac acaatcaaag acgcaattga aaacggtaga accctctatg attttttaag
     1861 aggtaacgaa gagtacaaat ttagacttgg cggtatacca aaaccaattt acgatttgag
     1921 tttctcaatt aaataacagt gagttactta gaaacaaaaa atcccgcgat aagcgggact
     1981 ttccgactga tttcaatgtg gcttgattta cctagtttga gcacatttac tatctcaaaa
     2041 aattataaaa aaacaagagt cacttttcaa tttttcgtgg aggcgacggg aatcgaaccc
     2101 gtaaaccagg ttgctcaaac taggttcaag accacttgcg cc
//
LOCUS       DAOQZP010000108         2117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_60205_length_2117_cov_3.456353, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000108 DAOQZP010000000
VERSION     DAOQZP010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60205_length_2117_cov_3.456353"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PKH60_05270"
     CDS             <1..445
                     /locus_tag="PKH60_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17480.1"
                     /translation="KTFRMISTERDFEYLTLEQAKALNSASERDFALRCLAVGCLLVH
                     QIPVGSSVIDFLVINPKRYTNAGGETRGKLVEVTQLDESPERQINSTNKRRKSRNKNK
                     IKQQYQKTKARKERQVDAMRSSGHPWTILYNEQLKNMKRAARAKR"
     gene            complement(714..2018)
                     /locus_tag="PKH60_05275"
     CDS             complement(714..2018)
                     /locus_tag="PKH60_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17481.1"
                     /translation="MLEITPLSRRHILGLRETPHLPKLTNPLADPPEQEPSQPEGVPK
                     SVEDAGELFAFVLLALGAYHNSQDVASFYSVQSESLARDLPRFKEMVAQLGEFGKQFA
                     QLHTETSELLTAWTNAYRKKDSDGDPYWREPSQLTATNLSSSTITQLEALAVKLSSRS
                     VIALDQAPQSFDFRPDASNLYYTSHEEDQKLSRVTAGLSVVALAGAFALYEEVIAKAS
                     TAIFMEHRPLIDPDHGVRRRTALKIIAAFLGSYIAYKAHQKFMLQNVVYLDTLQNHAQ
                     SIVQKINTPDEENFERYFSISLDDLYSQLNEYRDTLEAALNSGYDPHQGILSLDGHWP
                     TIQQHLASTLATITTTLSYLEKTFAMGSGKPTIPPDFSAIINSLWATEQIQIKSQTME
                     GQVVNRHWINAAISAVGMGGLAVLTEAVIIPLTDALSQSSSR"
BASE COUNT          598 a          418 c          503 g          598 t
ORIGIN      
        1 gaaaactttc cgtatgatct caactgagcg agattttgaa tatctgacgt tagaacaagc
       61 aaaagcgcta aattctgctt cggagagaga tttcgcactt cgttgtttgg cagtaggttg
      121 cctacttgtt catcaaattc ctgttggctc gtcagtaata gattttttgg taattaatcc
      181 gaagcgttat acaaatgcgg gcggtgaaac cagaggaaaa cttgtggaag tgactcagct
      241 tgacgagtcg ccagaaaggc aaattaattc aaccaataag cgacggaaaa gtagaaacaa
      301 aaataaaatc aagcaacaat atcaaaagac aaaagctaga aaagagaggc aagttgatgc
      361 aatgaggtct tcgggacacc cttggaccat tttgtataac gaacaactca aaaacatgaa
      421 gcgagcggca agagcaaaac gctagcaatt acataattaa ctttctgtta gtataaatac
      481 atggatttgt ttaatctttc cactgggttg tggagcattt tcgtatttct atacttttca
      541 acgaaaaatt attttttagc aagaattttt caccatccgg aatttaaatt gccggtgtgg
      601 ttaacgacaa ttatctctat tgctgccttg atcgtgaatg tctttcttca atctgaatac
      661 gaacgatact taaacctctc ttatattaaa ggctctatac ccaataaaag tcgctatctg
      721 cttgaagact gagaaagcgc atcagtgagt ggaatgatga cggcctcagt cagtactgcc
      781 aagccaccca tgccaactgc actaattgcc gcattgatcc agtgtcgatt gactacttgt
      841 ccttccattg tctggctttt aatctgaatt tgctcagtag cccaaagaga gttgataatt
      901 gcagaaaaat ctgggggtat ggtgggtttt ccactaccca tggcaaaggt tttttctaga
      961 tagctgagtg ttgttgttat ggtagcaagc gttgaggcaa gatgttgctg gatagttggc
     1021 cagtgaccat caagagaaag aattccctgg tgaggatcat atccagagtt cagagctgct
     1081 tcaagagtat cgcgatactc attgagttga gagtacagat catcgagaga gattgaaaaa
     1141 taccgttcaa aattttcttc gtctggagta ttgatttttt gaactattga ttgtgcgtgg
     1201 ttttgcaaag tatccaaata cacaacattt tgcaacatga acttttggtg agctttgtag
     1261 gctatgtagg aacccaagaa cgcagctata attttgagag ctgttctgcg acgcactcca
     1321 tgatcgggat cgataagggg tctgtgttcc ataaagattg ctgttgaggc tttggctatg
     1381 acttcctcgt acagagcaaa agcacctgca agggctacaa cagaaaggcc ggcggtaact
     1441 ctgctcagtt tttgatcttc ttcgtgcgac gtgtaataga gattagaagc atctggccga
     1501 aaatcaaagc tctgtggggc ttggtcgagc gctatgactg atctgctgga tagtttcact
     1561 gccaacgcct ctaactgagt aatggtgctg cttgagagat tggtggcagt gagctgagac
     1621 ggctcgcgcc aataggggtc cccatcgctg tctttttttc ggtaggcatt ggtccaggcg
     1681 gtgagtagct cactggtttc agtatgaagt tgagcaaact gtttaccaaa ttctccaagc
     1741 tgtgcgacca tttctttgaa tcgaggtaag tcgcgggcga gcgactcact ttgcacagaa
     1801 taaaagctgg cgacatcttg agagttatgg taagcaccga gcgcgagtaa cacaaatgca
     1861 aagagttccc cagcgtcttc tacagacttt ggaactccct cgggctgaga cggctcctgc
     1921 tctggtggat cagcgagtgg attggtaagt tttggcaaat gaggtgtctc gcgaagacct
     1981 aagatgtgcc ttcgagatag tggagtgatt tctaacataa aaacgacttt gttgcagact
     2041 ataaaaatat catagcatct agaacgcaaa aagccccacc ttgcggtggg gcctttcgct
     2101 cttcagtttt gcttgct
//
LOCUS       DAOQZP010000109         2110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_60507_length_2110_cov_5.165450, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000109 DAOQZP010000000
VERSION     DAOQZP010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60507_length_2110_cov_5.165450"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PKH60_05280"
     CDS             complement(<1..574)
                     /locus_tag="PKH60_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17482.1"
                     /translation="MKNSLKLLSRYWVLVLLVSLYVLNRLPLLFFQTFAFTFDHGKDS
                     LAVMDLVTSLSPKLIGPWTSIPGLYFGPGWYYLLAPLYWLLNFHPFAGPLTMMLLGLV
                     QLMVVYKYFGKLAALILTSAPIWFITTTNASNPYPLTLVTWVILILLEKIKKYPKKTT
                     FWLLLGASIGMGFHFSTAFAVLYVVLVPISF"
     gene            653..1354
                     /locus_tag="PKH60_05285"
     CDS             653..1354
                     /locus_tag="PKH60_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17483.1"
                     /translation="MHVFPPFLQKIMFSLVFVLSVTAVVTIFYTLFINVNQTPTSQYL
                     IADIPTVSPTYPPMIFPTETTAPTPDIEPTNTIDPTNTPLVSIPRISTVGWKSVSNNG
                     VNFLIPPDARCSGTDDACIGVVTSFIYEGKTIESNMIIAVEPYRGGSRRTQFIEDHEQ
                     SLVESCNPIFVESLFGSVNALQIASSYDCYGAGGIVTVVGDKLVTFYALSYDPQTNEI
                     ERSPIRDTIISTVSN"
     gene            1493..>2110
                     /locus_tag="PKH60_05290"
     CDS             1493..>2110
                     /locus_tag="PKH60_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17484.1"
                     /translation="MNKRVRRFLLQVFSSLNALFLTFQPLAALPVLLANPVVVQAEAS
                     VSADAVDLTFDAGSHEFQLRVKTDQALPYVLTYLDEKQDPQVEQAAQGNLEKMDEGVF
                     GADVYAGTCSGEVCTPITFLRGTLAVGESFKNSFQLINDVIVFDGSDTTVEQVCVNLD
                     EVGMTAGDTLWSVDETTGVAETKTKVQPGVSYQFPLDDAVSVTFSC"
BASE COUNT          610 a          400 c          467 g          633 t
ORIGIN      
        1 aaaaactaat tggaaccaat actacatata aaacagcaaa tgcggtggaa aaatggaagc
       61 ccatgccaat acttgcccct aataataacc aaaacgtcgt tttctttgga tattttttaa
      121 ttttctctaa gagtattaaa attacccaag ttaccaaagt gagtggatag ggatttgagg
      181 cgttggtagt ggtaataaac caaatgggtg cagacgtcag tatgagagcc gcaagtttgc
      241 caaaatattt atagaccacc atcagttgaa ccaagcctag tagcatcatg gtgagtgggc
      301 cagcgaacgg atggaagttt agcagccaat acagtggggc gagcaaataa taccagcctg
      361 gcccaaagta taatcctggt atgctggtcc aggggccaat caattttggt gaaagactgg
      421 tgactaaatc catcacagct aacgaatctt tgccatgatc aaaggtaaaa gcgaacgttt
      481 ggaaaaatag taggggtaat cggttcaaga catagagcga aactagtaga actagcaccc
      541 aatatcttga aagtagtttg aggctatttt tcattacctg ttagtactat atccaatagt
      601 aacaaaaaaa aatactggat tctctaattg agtttggagt ataattttca ccatgcacgt
      661 ttttccacct tttttacaga agatcatgtt ttcattggtg tttgtacttt ctgttactgc
      721 agttgtgacc attttttata ctctgtttat taatgtaaac caaactccaa ccagtcaata
      781 cttgattgca gatataccaa ccgtttcacc tacgtaccca ccgatgattt ttccgactga
      841 aactacggca ccaactccag atattgagcc aaccaacacg attgacccaa ctaatactcc
      901 tctggtatct atacctagaa tttccacagt tggttggaag agtgtatcaa acaatggtgt
      961 taattttttg atacccccag atgctcgctg ttctggaact gacgatgctt gtattggcgt
     1021 agttacttca ttcatatatg aagggaaaac gatagaatcc aacatgatta ttgcagtcga
     1081 accctatcgt ggtggctcaa gaagaactca atttattgag gatcacgagc agtctttggt
     1141 tgaatcatgt aatcccattt ttgttgaatc tctttttggt tcagtcaacg ctctgcaaat
     1201 tgcatcatcc tatgactgct atggtgcagg tggaattgtg actgtggttg gagataaatt
     1261 agtcacattt tatgcgttat cgtacgatcc acaaaccaat gaaattgagc gttctccgat
     1321 tagggatacg attatttcaa cagtatctaa ttaaatttag tcagttatca ctagatttta
     1381 cgactacttg cagctcagta gaatagtagt acaataagat ttaacacaca taatgtgtag
     1441 tgtggtgttg ggtcgaccag cctgcggtcg acgcgattaa aggaatatcc ttatgaacaa
     1501 acgcgtccgt cgtttcctgt tgcaagtatt tagttccctc aatgctctct tcctcacgtt
     1561 ccaaccgctt gcggcgttgc cggtgttgtt ggctaatcct gtggtggtac aagctgaagc
     1621 gagtgtgagt gccgatgcag tggatttgac gtttgacgcg ggtagtcatg aatttcagtt
     1681 acgagttaag acggatcagg cgttgcccta cgttttgacg tatctggatg aaaaacaaga
     1741 tcctcaggtt gagcaagccg ctcaaggtaa cttagagaaa atggacgaag gtgtgtttgg
     1801 tgccgatgtc tatgcgggca cttgttcagg tgaagtttgt acaccaatta cttttctcag
     1861 agggactttg gctgttggcg aatcatttaa gaattcattt caattaatca atgatgtgat
     1921 tgtgtttgat ggatctgata ctaccgtaga gcaagtttgc gtaaatttgg atgaagtggg
     1981 catgactgct ggcgatacat tatggagtgt agatgaaact actggtgtag cagaaaccaa
     2041 aaccaaggtt cagcctggtg tgagttatca atttcctctg gatgacgctg tcagcgtgac
     2101 tttttcgtgt
//
LOCUS       DAOQZP010000110         2084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_61705_length_2084_cov_4.787087, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000110 DAOQZP010000000
VERSION     DAOQZP010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61705_length_2084_cov_4.787087"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1409
                     /gene="ftsH"
                     /locus_tag="PKH60_05295"
     CDS             <1..1409
                     /gene="ftsH"
                     /locus_tag="PKH60_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNQ17485.1"
                     /translation="ADGIMGIGRSKAKIFIKGKQNTTFANVGGMDEAKKELEEIVDFL
                     KNPKKYQKLGARTPKGVLLVGPAGTGKTLLARAVAGEANVQFLSIAGSEFMEMLVGVG
                     ASRARDLFETAKKLAPSIIFIDEIDAIGRVRGMGSMGGHDEREQTLNQILVEMDGFTQ
                     NDNVIVMAATNRGDMLDPALVRPGRFDRRVVVNLPDLEERKYILSIHAKGKPVDKDLN
                     WEKVAKRTVGFSGADIENMLNEAAIAVARENRESMTMADIEEASLKVKLGPSKRRLQD
                     DTERKMTAYHEAGHALIAHVSPHADPVHRISIVSRGRALGYTLTPPERDKLQTTKSEL
                     LDEIAVLLGGRAAEQLVFNELTAGASSDIERATKVARAMVMDFGMSDLGPMQFSPQYD
                     LSDYGRSWGEPLKVSDKLQEQVDGAVKNFVDEGLKKAIALLKKYRKQLDVVSEKLLEQ
                     ESLDGDEFAQLMGMKKVA"
     gene            complement(1419..1967)
                     /locus_tag="PKH60_05300"
     CDS             complement(1419..1967)
                     /locus_tag="PKH60_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17486.1"
                     /translation="MNFAEVNTTSVLEPGTLSPGIARALIEKGEAMPSIESVISKIDS
                     LLQSELKIFIDELVHTFGGEQIKGYISRESVSGVMTAIGDRLRHVVEANLKPYEASIS
                     FRLIEVTDQFSGIMHYQRNNAATIEEIRSMYRLNLKEGQLALGLFRNKTLPYHYELRF
                     CVINSLTGQLQQAAGQEEVRVI"
BASE COUNT          586 a          420 c          503 g          575 t
ORIGIN      
        1 gggctgatgg catcatggga attggtcgca gcaaagctaa aatatttatc aagggcaagc
       61 aaaatactac ctttgcgaat gttggtggta tggatgaagc aaagaaagag cttgaagaaa
      121 tagtcgattt cttgaaaaat ccaaagaaat atcagaagct gggcgcgcgc acaccaaagg
      181 gcgtgttact tgttggtcca gctggaacgg gtaaaacatt gttggcgcgc gccgtcgctg
      241 gtgaagcaaa tgtccaattt ttgtctattg ccggtagtga atttatggag atgctggtgg
      301 gagtaggtgc ttccagagct agagatttgt ttgaaacagc taaaaaactt gccccatcaa
      361 ttatttttat tgatgaaatt gatgccattg gccgagtccg tggcatgggc agtatgggcg
      421 gccatgatga gcgcgagcaa actttgaatc aaatattagt agagatggat ggctttaccc
      481 aaaatgataa cgtcatagta atggcagcta ccaatcgggg cgacatgctc gatcctgctc
      541 tggtcagacc cggtcggttt gatcgtcgcg tcgtagttaa tctgccagac ttagaagaac
      601 gtaaatacat tctttccatt cacgccaaag gcaagccagt cgataaagat ctcaattggg
      661 aaaaggttgc caagcgcacc gttggctttt ctggcgctga tattgaaaac atgcttaatg
      721 aggctgcgat tgcggtggca cgggaaaacc gcgaaagcat gactatggcc gatattgagg
      781 aggcctcgct taaagttaag ctcggcccca gcaagcgtcg tttgcaggat gacactgagc
      841 ggaaaatgac tgcttatcat gaagcaggtc atgcactcat tgctcatgtg agtccacacg
      901 ccgatcctgt gcatcgcatt tcaatagttt cgcgaggacg agcgttgggt tacacgctca
      961 ctccgccaga aagggataaa ttacagacga cgaaatcaga attactggat gaaattgcgg
     1021 tgttacttgg tggtcgcgca gccgaacaat tagtgtttaa tgaattaacg gctggtgcaa
     1081 gcagtgacat tgagcgagct actaaggtgg cgcgtgccat ggtaatggac ttcggtatgt
     1141 ccgaccttgg cccaatgcag ttttcaccac agtacgattt atctgactat ggccgatctt
     1201 ggggcgaacc actcaaagtt tctgacaaac ttcaagaaca agttgatgga gcagtgaaaa
     1261 acttcgttga tgagggtctg aaaaaagcga ttgcgttgtt gaaaaaatat cgcaaacagc
     1321 ttgatgttgt ttcagaaaaa ttgctcgagc aagaaagcct tgatggtgac gaatttgctc
     1381 agttgatggg gatgaagaaa gtagcttaga gaacctttct agattactct gacttcttct
     1441 tgaccagcag cttgttggag ttgtcctgtc agagagttta taacacaaaa acgcaactcg
     1501 taatgatagg gtagcgtttt gtttctgaat aatccgagcg ctagttgtcc ttctttcaag
     1561 tttagtctat acatactacg aatctcttca atggttgctg cattattgcg ttgatagtgc
     1621 atgatgcctg agaactggtc ggttacctca ataagcctaa atgaaataga agcctcatat
     1681 ggttttaagt tagcttcaac aacatgtctt aaacggtcgc caatggctgt cataacccca
     1741 gagacacttt cgcgactaat ataacccttg atttgttcac cgccaaatgt atgtacgagt
     1801 tcgtcaataa atatttttaa ttcagattgt agtagcgagt cgattttcga aataactgac
     1861 tcaattgatg gcatcgcctc gcctttttca attagagctc ttgctatgcc aggggacaaa
     1921 gtacctggct ccagaacact agttgtattc acttcagcaa aattcatatt ttctcctttt
     1981 tggggaaaat atactatagg attaaattct gtcaaattca ccgcttactg cattcgcacc
     2041 cgaagtgcac cactttgtct gttaactact acatgattgt atct
//
LOCUS       DAOQZP010000111         2033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_64210_length_2033_cov_3.455005, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000111 DAOQZP010000000
VERSION     DAOQZP010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64210_length_2033_cov_3.455005"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PKH60_05305"
     CDS             complement(<1..249)
                     /locus_tag="PKH60_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004836975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNQ17487.1"
                     /translation="MKFNLKSNYQPTGDQPLAIKQLVKGIVDHKKRQVLLGVTGSGKT
                     FTMANVIQQTQLPTLVIAHNKTLAAQLYQEFRDYFPDNA"
     gene            291..1049
                     /locus_tag="PKH60_05310"
     CDS             291..1049
                     /locus_tag="PKH60_05310"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HNQ17488.1"
                     /translation="MKVTPIKTSIVKPHDDLEAFLSAHTPKLVERSILSVTSKIVALC
                     EGRVVPKISGEKEEKHQIARQEAEKYLDPNGSKYNLMLTIKTGVMAVNAGVDESNVDP
                     DHYVLLPANSFLSAERIWYWGRKYFKIKEFGVIITDSRTVPLKWGVLGTALGYCGFVG
                     LRNEIGKPDLYGRPMHMTQVNNAEALAVTSVYIGGETAESQPLVLIEDVPQIQFTQTP
                     PSAREQRSLHIKPEEDVYWPIISKADWLAGGSKS"
     gene            1046..1759
                     /locus_tag="PKH60_05315"
     CDS             1046..1759
                     /locus_tag="PKH60_05315"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNQ17489.1"
                     /translation="MTFLFVFAHPDDETVACAGTIHKLVKSGYKVIVVSATNGSAGQV
                     TQAVQKPLASKYRGDLGALREAEFHKVCKHLGVYQSQLLQFADGQITNEEVWGELTTK
                     IAELINYHRPEVLVTFDHSGWYYHLDHVGVSIATTLAFHQTKSPQLLLFSNFQPQQNR
                     WQYVFPDPTLSTHQVVVEDVEHKLKALQIHESQDLEVVKQHILSQSPHVENYQLAFTK
                     ETNKKILKNITDIFQEITT"
BASE COUNT          612 a          393 c          447 g          581 t
ORIGIN      
        1 cgcgttatct ggaaagtaat cccgaaactc ttggtagagt tgagcagcca gcgttttgtt
       61 gtgagcaatt accaatgtgg gtaattgtgt ttgctgaatt acattagcca tggtgaaggt
      121 tttgcctgag ccagtcacgc caagtaacac ctggcgtttt ttgtgatcta caataccttt
      181 caccagctgt ttaattgcaa gcggctggtc accagttggc tggtagtttg atttaagatt
      241 aaatttcata tttaaagtgt tttgcgctga tgtattgtat gatagcaccc atgaaagtga
      301 cgccaataaa aaccagtatt gtgaaacctc atgatgacct agaagcgttt ctatcagccc
      361 acacacccaa acttgtcgaa cggagtattt tatcagttac aagcaagatt gtggcgttgt
      421 gtgaagggcg ggttgtgcca aaaattagcg gtgagaaaga ggaaaaacat caaatagctc
      481 gtcaagaggc tgaaaaatac ttggatccca acggatcaaa atataatttg atgctcacca
      541 ttaagactgg agtaatggct gttaacgccg gagtagatga atccaatgta gacccagatc
      601 actatgtttt attacctgcg aattcatttt tgtcggcaga aagaatctgg tactggggac
      661 gaaagtattt taaaattaaa gaattcggag tgatcattac tgacagtcgc actgtcccac
      721 ttaagtgggg tgttttaggc acagccttgg gatattgtgg ttttgttggt ctgcgtaatg
      781 aaattggtaa gccagacttg tacggtcggc caatgcatat gacgcaagtg aataacgcgg
      841 aggctttggc agttacttcc gtatacattg gaggcgaaac agcagaatct caacccctag
      901 ttttaattga ggatgtgccg caaatacaat ttactcaaac ccccccatcg gctagagaac
      961 aacgatcgtt acatattaaa cctgaggaag atgtctattg gccgatcatc tctaaagcgg
     1021 attggcttgc gggaggtagc aaatcatgac gtttcttttt gttttcgctc acccggacga
     1081 cgagaccgta gcttgtgctg gaacgatcca caagctggtc aaaagtgggt acaaagtaat
     1141 agttgtttca gcaactaatg gttcggctgg tcaagtcact caagcagttc agaaaccact
     1201 ggcttcaaaa tatcgaggag atcttggagc attgcgtgaa gctgaatttc acaaagtgtg
     1261 caaacacctg ggagtctatc aatctcaatt actacaattc gctgatggtc aaataacgaa
     1321 tgaagaggtt tggggggagc tcaccacaaa aattgctgaa ctaatcaatt accatcggcc
     1381 agaggttcta gttacctttg accactctgg ttggtattac caccttgatc acgtgggtgt
     1441 ttccattgcc accaccctag ccttccatca aacaaaatca cctcaactac tgcttttttc
     1501 taatttccag cctcagcaaa accggtggca gtatgttttt ccggatccca ctttgtctac
     1561 ccatcaagtg gtcgtggaag atgttgagca caaactaaag gcattacaaa tacatgaatc
     1621 tcaagatttg gaggtggtga aacagcacat tctctcccaa tcgccacatg tggaaaatta
     1681 tcaacttgct tttacaaaag aaacaaataa aaaaatcctt aagaatatta ctgatatttt
     1741 ccaggaaatt acaacttagg tgagtctaga ttatctttat agagcaattg atgttttgaa
     1801 aaggtaaaat gtggcatttc tatacctgaa aagtgagaaa aacgtgatgg ttcgtaaacc
     1861 aacaaactga tattgagagt gaaacaagtt gctacaaaac aactactgag ttttatttga
     1921 tggtttagtg taaagcacgg ctccagacag ggttatgctt tacactaaac catagttgta
     1981 tttgttgaat ttgatggtat aattgctcct actattgttg gagttaaatt aac
//
LOCUS       DAOQZP010000112         2003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_65814_length_2003_cov_4.237680, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000112 DAOQZP010000000
VERSION     DAOQZP010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65814_length_2003_cov_4.237680"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="PKH60_05320"
     CDS             complement(<1..1005)
                     /locus_tag="PKH60_05320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNQ17490.1"
                     /translation="MKTDKSKKTTIFILMFVMLINALSYGTIIPLLYPYSARFGIDPT
                     GLSLLFASFSFFQFLATPIIGRLSDRYGRKPLLVFSLLGTSISLALFASAQGVVMLFL
                     ARILDGITGGNNSVAQAVIADTHKPKERAQAFGLLGAAFGVGFLIGPAMGGLLSRYGL
                     ATPFWFASGLALVGTVLAFWLLPETRVKSIKTLQIKSVKVLDFPRLWHALWTPVTGIL
                     LLITLITSIGQNSFILGFQSVTVDVLQFSPTQVGLIFTAFGLVNVLMQGFGIRYLLKI
                     ATSQQLLFFSLAAGIAMVGLLGFVTTAPLFLIVLGLYMWIPPAAPFLSSLLSLATEEK
                     D"
     gene            1069..1152
                     /locus_tag="PKH60_05325"
     tRNA            1069..1152
                     /locus_tag="PKH60_05325"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1103..1105,aa:Leu,seq:taa)
     gene            complement(1162..>2003)
                     /locus_tag="PKH60_05330"
     CDS             complement(1162..>2003)
                     /locus_tag="PKH60_05330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNQ17491.1"
                     /translation="TLLFGGDMMFDRHIREKAQAKGNYDFIFDSLAPLFSEADVIVAN
                     LEGPVTDFPSRSVGSEIGSTNNYFFTFEPKVVVEILAQWPFIVNLGNNHILNFGQEGL
                     TQTYWYLKESGLPYFGYTQPAQEEPSYVIKEIRGISFGFVNYNQFVTGGKERVYRDMA
                     EIRDSVEVLVVYTHWGNEYVQENQVIKDLAHQLVEAGADLIIGSHPHVVQGVEAYEGK
                     RIYYSLGNFVFDQYFEEAVQNGLLVEAKVCQSTDTDSFNWEFIDHPIQLNLDGTTSLT
                     VVE"
BASE COUNT          651 a          457 c          383 g          512 t
ORIGIN      
        1 gtctttttcc tctgtagcaa gtgaaagaag actggaaaga aacggcgcag caggtgggat
       61 ccacatatat agccccaaga cgatcaaaaa gagtggggcc gtggtcacaa aaccaagtaa
      121 acctaccatc gctattcccg ccgccaaaga aaaaaacaat aattgttgac tggtggcgat
      181 ttttaacaaa taacgaatac caaaaccctg cattaaaaca ttcactaatc caaaggctgt
      241 aaaaattaga cctacttgcg ttggtgaaaa ctgcaacaca tcaacagtta ctgactggaa
      301 tccaagaata aacgaatttt gaccaattga agttatcagt gtgattagta aaaggatacc
      361 tgtaactggc gtccaaagcg catgccataa tctaggaaaa tcgagcactt tcacactttt
      421 aatctgcaaa gtttttattg acttaactcg agtttcaggc aataaccaaa aagccaagac
      481 tgttcccaca agtgctagcc ctgaggcaaa ccaaaatggc gtagcaagcc cataccgact
      541 gagtaaacct cccatcgctg gcccaattaa aaatccaacc ccaaaggcag ctccaagcaa
      601 accaaaagct tgagctcgct ctttcggttt gtgggtatcg gcaattaccg cctgagccac
      661 cgaattgtta ccaccagtaa tgccatctaa aatccgtgcc agaaataaca ttactactcc
      721 ctgagcgctt gcaaacaagg caagcgagat actcgttccc aacaaactga aaaccagtaa
      781 cggcttcctc ccgtagcgat cagataaacg cccaatgatt ggggttgcta aaaattgaaa
      841 aaacgaaaag gaggcaaata acaaacttag cccagtagga tcaatgccaa acctcgcaga
      901 atatggatag agcaaaggaa taatagtgcc ataactcaat gcatttatca gcattacgaa
      961 catgaggatg aaaatagttg tttttttgct tttatctgtt ttcattgaag gattctgatt
     1021 gtactcgcga cactcggttc tgtcagtgta taattatccc cacacctcgc cgaggtggcg
     1081 gaattggtag acgcgtcaga cttaaaatct ggtggtagca atatcgtgcg ggttcgattc
     1141 ccgccctcgg cacataaaaa tttattcaac aactgtcaat gaggttgtcc catcaagatt
     1201 aagttgaatt ggatgatcaa taaattccca attgaaagaa tcggtgtcag tcgattgaca
     1261 aacttttgct tcaactaaca atccattttg caccgcttct tcaaagtact gatcgaatac
     1321 aaaattgcca agagagtaat aaatccgctt tccttcgtaa gcttcaacac cttgaaccac
     1381 atgaggatga gagccgataa ttaaatctgc cccagcttca acaagttgat gagcgagatc
     1441 ttttatcacc tggttttctt ggacatactc gttgccccaa tgggtataga ccaccagtac
     1501 ctcaactgaa tctctaattt cagccatatc gcgataaact cgttctttcc caccagtcac
     1561 aaattggttg taattgacaa agccaaagga aatgccacgt atctccttga ttacatagct
     1621 gggttcttct tgagcgggct gagtataccc aaagtaaggt aaacccgatt ctttcaaata
     1681 ccagtacgtt tgggttaatc cttcttgacc aaaattcaaa atgtgattat tacctaaatt
     1741 cacgatgaat ggccattggg ctaagatttc gacgacaact tttggctcaa aggtaaaaaa
     1801 atagttattt gttgagccaa tttcgcttcc aacacttctc gaaggaaaat ctgtcaccgg
     1861 accctcaaga tttgcgacaa tcacatcagc ttcagagaaa agcggggcca gggaatcaaa
     1921 aataaaatcg tagttacctt ttgcttgggc tttttctctg atgtgacgat caaacatcat
     1981 atcgcctcca aaaagtaatg tgg
//
LOCUS       DAOQZP010000113         1877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_73264_length_1877_cov_4.835346, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000113 DAOQZP010000000
VERSION     DAOQZP010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73264_length_1877_cov_4.835346"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PKH60_05335"
     CDS             complement(<1..841)
                     /locus_tag="PKH60_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNQ17492.1"
                     /translation="MTKRYSQTQYRSLYEESIASPTKFWQRYAVTEIEWFQPFITAFT
                     WKYPHYSWFDGGMINITHNCLDRHIAHGNGDRIAYIYNNELEEEESITYAQLLARVNQ
                     VANMLHKYGIHKGDRVVIYMPLTIDQIAIMLACARMGAIHSVVYAGFSAEALAIRIKD
                     TQAKIICTATWMQKNGKQHDLLMVVREAVKMCPSVKHVIVMQRKTRPMKLQTGESDLH
                     ELLDAQSKEFAVVPVESSTPLFILYTSGTTGTPKGIVHAHGGYNLYSHMTFKYNFDIQ
                     ENQV"
     gene            complement(909..1682)
                     /locus_tag="PKH60_05340"
     CDS             complement(909..1682)
                     /locus_tag="PKH60_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNQ17493.1"
                     /translation="MKPFAITMSGGSLGCLAYASFLETLKKEYNLEPSLLAGLSGGAV
                     VAPLISAGCSAEEIYQIIKNLLKMTMLAHHIRHFELIEHHKAIEYFRKVLPIKKFEAL
                     PIPVLIFSSNIVQQTTEVIDSGDIASAIVGSCSIYPALRPIKRLGKLLMDGGYTTFYG
                     AKFIRERGFSKVVGIDVTGINEGNMPLFLRQFIRPLNTSVAQVKQYELQSYPVDLDIR
                     LSVRAPIFYELEKKKDWILQSGVSAAHEYFPKNKKLLSN"
     gene            complement(1693..>1877)
                     /locus_tag="PKH60_05345"
     CDS             complement(1693..>1877)
                     /locus_tag="PKH60_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNQ17494.1"
                     /translation="AETLAKSQNLKLGKIQQITEMGATNVCPIMYDRAMGGGGGPAEA
                     GTSTLSKTVSVTFEIR"
BASE COUNT          520 a          413 c          356 g          588 t
ORIGIN      
        1 gaacttgatt ttcttgaata tcaaagttgt acttgaacgt catatgagag tacaagttgt
       61 agccaccatg agcatgcacg attccttttg gggtgccagt cgtgccagat gtatacaaaa
      121 taaagagagg tgtagaactt tcgactggca ccaccgcaaa ttcctttgat tgggcatcta
      181 gtaactcatg aagatcggat tcaccagttt gtaatttcat cggccgagtt ttgcgctgca
      241 tgactatcac atgctttact gaaggacaca tcttgaccgc ctcacgaacc accataagca
      301 aatcgtgttg tttaccattt ttttgcatcc aagttgccgt acagattatc ttggcttgcg
      361 tatcttttat tcgtatggct aatgcttccg cgctaaaccc tgcgtaaact accgagtgaa
      421 tcgcacccat cctggcgcag gccaacatga tagcgatctg atcaattgtg agcggcatat
      481 agatcaccac tcgatccccc ttgtgaatac catatttatg taacatgttg gctacttggt
      541 tgactcttgc gagtagttga gcgtaggtaa tgctctcttc ttcttctagc tcgttgttat
      601 agatgtaggc aatgcggtcc ccattgccat gcgcaatgtg tctatccaaa caattgtgag
      661 tgatattgat catgccaccg tcgaaccaag agtagtgagg atacttccaa gtgaacgcag
      721 taataaacgg ctgaaaccac tcaatctcgg ttacagcata gcgttgccaa aattttgtcg
      781 gacttgcaat tgactcttcg tacaacgatc gatactgagt ttgagaatag cgttttgtca
      841 ttgtagtctg taacttattc ttaaatttca cactgcttag tggtcatcgg ttgctaacta
      901 ataaaaaatc aattcgacaa cagtttttta tttttcggaa aatattcatg agcggctgat
      961 actccacttt gcagtatcca atctttcttt ttctctagtt cataaaaaat cggagcccta
     1021 acagagagtc taatatccaa gtccactgga tacgattgaa gctcatattg ttttacttgg
     1081 gccacagagg tgttaagcgg acgaatgaat tgtctcaaga aaagtggcat gttaccttca
     1141 ttaattccag ttacatctat acccactact tttgaaaaac ctctttctct aataaatttt
     1201 gcaccgtaaa aagttgtata cccaccgtcc attagtaatt ttcccaatct tttgattggt
     1261 ctcaatgccg gatagataga acaactaccc acaattgccg atgcaatatc tccagaatct
     1321 atcacttcgg tcgtttgttg aacgatattg ctcgaaaata tcaaaactgg tattggtaaa
     1381 gcttcaaatt tttttattgg taggactttt ctaaagtact caattgcctt gtgatgctct
     1441 ataagctcaa agtgcctgat atgatgagcc aacattgtca ttttaagcaa gttcttaatt
     1501 atttgatata tttcctcagc agaacatcct gcggatatga gtggtgcgac aacagctcca
     1561 ccactcaatc cagctagcaa tgaaggttcc agattgtatt ctttttttaa tgtttctaga
     1621 aaactagcat aggcaagaca ccctaggctg cctccagaca tcgtaatcgc aaatggtttc
     1681 atccagataa tcctaacgta tctcaaacgt tacactaact gttttactga gtgtagaagt
     1741 tccggcttca gcaggaccac cgccgccacc catggctcga tcatacataa ttgggcaaac
     1801 attggtcgca cccatctctg tgatttgttg aattttacca agtttgagat tttgactttt
     1861 ggctaaagtc tcagcct
//
LOCUS       DAOQZP010000114         1819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_77177_length_1819_cov_6.128118, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000114 DAOQZP010000000
VERSION     DAOQZP010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77177_length_1819_cov_6.128118"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PKH60_05350"
     CDS             <1..678
                     /locus_tag="PKH60_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HNQ17495.1"
                     /translation="RPTGQISDYYANLFAPAGVTFSIWGIIYLLLAGYVLYQFVNKDK
                     KIEKLIQTINPLFITTSLANILWIFAWHYDFIGISVLIMSVLLILLIKIADILRKVQF
                     TPREKLFIKAPFSVYFGWITVATIANITVFLVSVGWNGFGIAESVWTSIILVVGALIG
                     ILRMTKDKNIAYGLVLVWAYLGILLKHTSVTGFNGQYSSIIATVVVCVLLFVFSLGII
                     LYKHQKD"
     gene            682..1221
                     /locus_tag="PKH60_05355"
     CDS             682..1221
                     /locus_tag="PKH60_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNQ17496.1"
                     /translation="MKNVYHIVTHPNLQKSRINKAVLKAFEDKPGIETRKLYEVYPDW
                     KIDVTKEKQSLLKADILVVQTPFYWYSTPGLFKEWQDRVLEYGFAYGDGGDKLKGKQF
                     QIVLSAFGPEEAYQHDGYNHFTIEELLRPLEQTANLCQMVFLKPITLLGVAHKTDAEV
                     TAFKNLVEKEIETLMNLSN"
     gene            1254..1736
                     /locus_tag="PKH60_05360"
     CDS             1254..1736
                     /locus_tag="PKH60_05360"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNQ17497.1"
                     /translation="MRDGTLILISGLPGAGKTTLAKKLAAERSAIRLCPDDWIISIIK
                     DLNDIPERDRLRDPVEQLLWKQAQELLSLGMTVILENGFWSKAERDSYLATGKKLGAQ
                     VELHFVDAPFEELWKRVEKRNTNPNEFQMTKKELEDAFAAFQPPSTEEGKAYDYFNPH
                     "
BASE COUNT          566 a          360 c          362 g          531 t
ORIGIN      
        1 cgcccaactg ggcagatatc tgactactat gccaatctgt ttgctcccgc tggagtcacc
       61 ttttcgattt ggggcatcat ttacctttta cttgcgggat atgtgctcta ccagttcgtc
      121 aacaaagaca aaaaaattga aaaactgata caaacaatta atccattgtt tatcaccact
      181 tcactcgcaa atattttgtg gatctttgct tggcattatg attttatcgg catttcggtc
      241 ttaataatgt cggtgttact cattttgctt attaaaattg ccgacattct ccgcaaggta
      301 caatttactc cacgcgaaaa actgtttatc aaggcgccct tcagtgtcta ctttggctgg
      361 atcaccgtgg caacgattgc taatatcacg gtatttttag taagtgttgg ctggaatgga
      421 tttggtatcg ctgaatctgt ctggactagt atcatcttag tcgtcggcgc gctgattggc
      481 atactacgaa tgactaaaga taaaaacatt gcttatggtc tagttcttgt ttgggcatat
      541 cttggcatac tactcaaaca cacttctgta acaggcttca atggacaata ctccagcatt
      601 attgccaccg ttgtagtttg tgtgttacta tttgtgtttt ctttaggaat aatactatat
      661 aaacatcaga aagattaagg tttgaaaaac gtctaccaca tcgtcactca tccaaatttg
      721 caaaaatcgc gaataaataa agccgtactc aaagcttttg aagataaacc aggtattgag
      781 actagaaaac tttacgaagt atacccagac tggaaaattg atgttactaa agaaaaacaa
      841 agtttgctca aagctgacat actagtagtg caaacccctt tttattggta ttcaactcct
      901 ggcctgttta aagaatggca agaccgtgta ctagaatatg gctttgccta cggtgatggt
      961 ggagataaac taaagggcaa gcaatttcaa attgtacttt cggcttttgg tccagaagaa
     1021 gcgtatcaac atgatggtta caatcacttt acgattgaag aattactcag acccctcgaa
     1081 cagacggcca atctttgcca gatggttttt ctcaaaccca ttacgctgct tggggttgca
     1141 cataaaacag atgctgaagt aactgcattt aaaaatctgg tggaaaaaga aattgaaaca
     1201 ctaatgaatc tatcaaacta aattgctaac ctagataaaa aatagactca aatatgagag
     1261 atggaacctt aattcttatc agcggacttc ccggcgccgg caaaactact ctggcaaaaa
     1321 aactcgccgc tgaacgaagc gcaattagac tttgtcctga tgactggatt atttccatta
     1381 taaaagatct caatgatatt cctgagagag atcggttacg ggatccagtg gaacaactct
     1441 tatggaagca ggcgcaagaa ttacttagct taggtatgac ggtaatcttg gaaaacggtt
     1501 tttggtcgaa ggcagaacgt gactcatatc ttgccactgg taaaaaactt ggagctcagg
     1561 ttgagctaca tttcgttgac gctccttttg aagaactatg gaagcgcgta gaaaaaagaa
     1621 atactaaccc aaatgaattt cagatgacaa aaaaagaact tgaggatgct tttgcagcct
     1681 tccaaccacc atcaactgaa gagggaaaag cctacgatta ttttaaccct cattgagcga
     1741 gtagtttcat gactatggag atactaatta tctcggcttg agtggcaatt catgctgagc
     1801 gatcaacggt attacgttc
//
LOCUS       DAOQZP010000115         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_79003_length_1793_cov_5.341772, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000115 DAOQZP010000000
VERSION     DAOQZP010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79003_length_1793_cov_5.341772"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1604)
                     /locus_tag="PKH60_05365"
     CDS             complement(<1..1604)
                     /locus_tag="PKH60_05365"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNQ17498.1"
                     /translation="MYTLNTNLKEVRGIGAALQQRLILRNLFTVKDLLLQLPLRYEDR
                     RQILSITDVHKLGAEILKPVTIIAQVKNTSQQWRGRKSIQRATLFDQTGQMKVTWFNC
                     PFIIQTLKSEGQFYFSGVWNEKYQTLQQPVVERVTRQTAIHTARLVPLYSSVTGVSSG
                     WMRRIFTTLFDHLEIEDPLIKQAQTEVSLLDALRQLHFPEEPENVIAARQRIALEELL
                     SLMQQADQQKKQWAKIKAIKILENSSGAHQSQTLVDQLPFQLTNGQQQSLQDILADLT
                     QTKPMNRLLQGDVGSGKTVIALLAAQLCVTQGSTVCLLAPTRILAQQHLTTTQTLFPN
                     LPCELILGGTKSKQIGDESAAKLYIGTHALVSRISELQPALVIYDEQQRFGVVQRSSQ
                     TELKLNAKSTTPHVLTMTATPIPRSLMLTVFSHLTLSVIGEMPKERKPTITWYIPESK
                     RQGAYHWLAESIKEPNTLAMVVCPFIDPSTSESLENVAAAKDMYEKIVTQLPNNTKVA
                     LLHGKQKKDEQKAVIEAAFAGKIDVIV"
BASE COUNT          472 a          363 c          378 g          580 t
ORIGIN      
        1 acaattacgt caatttttcc ggcaaaggct gcctcgatta cagctttttg ctcatctttc
       61 ttttgtttgc catggagtaa agccaccttg gtgttattgg gaagctgagt aactattttt
      121 tcgtacatat ctttagccgc tgctacgttt tcgagagatt cagaggttga gggatcgatg
      181 aacgggcaca ctaccatggc taatgtattt ggttctttaa tcgactcagc cagccagtgg
      241 tatgcaccct gacgttttga ttctgggatg taccaagtaa tggtgggttt tctttctttg
      301 ggcatttccc caattacaga tagcgtgagg tgagaaaata ccgtcaacat caggctgcgc
      361 ggaatgggtg tggcagtcat ggtaagcaca tgaggcgttg tactttttgc atttagtttt
      421 aattcggttt gactacttct ttgtaccaca ccaaaccgtt gttgctcatc gtagataacg
      481 agcgcaggtt gtaattcact aattcgactg actaaagcat gagtacctat atagagtttg
      541 gcagcagact catctcctat ttgttttgac tttgtgcccc ccagtattaa ttcacaaggt
      601 agattgggaa ataaagtttg agtagtcgtt aagtgttgtt gggccaaaat cctggtagga
      661 gccaacaagc agacagtact tccttgcgtg acacacagtt gagccgccaa aagagcgatg
      721 accgttttac cagaccccac atccccctga agtaagcgat tcatcggttt tgtttgggtt
      781 agatcagcca aaatatcttg cagtgattgt tgttgaccat tagttagttg gaatggcaat
      841 tgatcgacca gcgtttgtga ttgatgagca cccgatgagt tttcaagaat cttgatagct
      901 ttaatttttg cccactgttt tttttgttga tcagcttgtt gcattaaact cagcaattct
      961 tcgagcgcta ttctttgtct ggcggcaatg acgttttctg gttcttcagg aaaatgtaat
     1021 tggcgtaatg cgtcaagaag tgaaacctct gtttgtgctt gtttgatgag tggatcttca
     1081 atttccaaat gatcaaacaa agtggtaaag atcctacgca tccagcctga agacacacca
     1141 gtaacagatg agtacaatgg aactaatctg gctgtatgaa ttgcagtttg tcttgttaca
     1201 cgctcaacta ccggttgctg gagtgtttga tatttttcat tccaaactcc actaaaataa
     1261 aattggcctt cactttttaa tgtctggata ataaatggac aattaaacca cgtgactttc
     1321 atctgaccag tttgatcaaa caaggttgcc cgctgtatac tttttctacc tctccattgt
     1381 tgcgaggtgt ttttaacttg agcaataatg gtaactggtt tcagaatttc agccccaagt
     1441 ttgtgcacat cggtaattga aagtatttga cgtcgatctt cataccgaag tggcagttgt
     1501 agcaataaat ctttgacggt aaaaagattt cggaggataa gccgctgttg gagtgcagcg
     1561 ccgatcccac gtacctcctt cagattagtg ttgagggtat acacaccaca atcctatcat
     1621 gaagttgctc aaagcatcgt cagaaaacta ccacccacca aactagctac catagcgatg
     1681 atcgcgatga ttgcaaaaat ttgaattaat ctttctcgct tcttttttgt catagttact
     1741 ttctattgta atttaaaata tcctcgttta ccaattttta aaatatcacc act
//
LOCUS       DAOQZP010000116         1764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_81153_length_1764_cov_4.283792, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000116 DAOQZP010000000
VERSION     DAOQZP010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81153_length_1764_cov_4.283792"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..430
                     /locus_tag="PKH60_05370"
     CDS             143..430
                     /locus_tag="PKH60_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17499.1"
                     /translation="MAGLIGGISGGTSPETVDSLRSSQMIMREGFERKFKTQQAEIER
                     LQDLYAVAQKEIQRLRKLPDENEAGSNYYEFLFWTVVIMVIIDKVIFYLAR"
     gene            487..705
                     /locus_tag="PKH60_05375"
     CDS             487..705
                     /locus_tag="PKH60_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNQ17500.1"
                     /translation="MKNKRILVAVMTGAILGIFCIIGVSVRLGFAGNEVFILATWVNR
                     VVMGLVIGLAPYYQSKLLQKILCFVGCF"
     gene            1021..>1764
                     /locus_tag="PKH60_05380"
     CDS             1021..>1764
                     /locus_tag="PKH60_05380"
                     /inference="COORDINATES: protein motif:HMM:NF013318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNQ17501.1"
                     /translation="MSYPMYPKTYVSEIVVGDKRIKIEIGKFSEQVSGAVLATCGETV
                     VHSTVALGRKVNLGYFPLSVEFAEKLYSAGTIKGSQWVKREGRPLDEEILSARVIDRT
                     LRPLFSDGITNEVQVINTVFSFDGENDPDMIGLVGSAVALAVSEIPFNGPVAGVRVGY
                     RKESGEFLLNPTHTERETSDLDLIVSGTGETIVMVEAGAKEVEESVVIEAMTKAQEQF
                     ASMCKQIAAIAKEVGKEKINLVDPEAEELT"
BASE COUNT          532 a          304 c          408 g          520 t
ORIGIN      
        1 acttttgaga gcgatacatc aaacatcttg acttagatta taccagaatg gagattaaac
       61 gggtgtttat tgcaattcag atctaatttg atagactgtg ttttagtttg tttactctgt
      121 agtttgatgc aaaaaggaag ctatggcagg tcttatcgga ggtattagtg gtggaacgag
      181 tccagagact gtagattcct tgcgtagttc tcaaatgata atgcgagaag gtttcgaaag
      241 aaaatttaag acgcaacaag ctgagattga acgactgcaa gatttgtatg cagtcgccca
      301 aaaagagatc cagcgacttc gaaagcttcc agatgaaaat gaggctgggt caaattatta
      361 cgaatttctt ttctggactg tagtaataat ggttatcata gacaaagtta tcttttattt
      421 agctagatag actgtttttt gctgttaatg aaaatttgac cttgtataat ctgagtaaga
      481 atacatatga aaaataaaag aatactagtt gctgtaatga ctggagcaat acttggaata
      541 ttttgcataa ttggagtttc ggttagactt ggttttgcag gaaacgaagt atttatcttg
      601 gcaacgtggg taaacagagt tgtgatgggt ttggtgattg gcttggctcc gtattatcaa
      661 tcaaaactac tacaaaaaat attgtgcttc gtgggttgtt tttagatttg taatcagtgg
      721 ctctttttat cttgcgaccg aatttagaga cacacctggt tttctggccg gacttgcgta
      781 tggcgtgatc atcgatttta ttgcaaccaa atacggacag aaaaaaatat aactgtgttt
      841 aactagattc gcagaaaaaa tttaacacta tgctatacta ccgccagtag ttagtaatat
      901 agtatctggg aaattaaccg gaggattcga gagagtagag gtcttgaaga cgtctcttct
      961 ccagaatact cgaaatttcc caataacgtc actttgcgtg acaccaagaa aggagtagga
     1021 atgtcctacc caatgtatcc aaaaacctac gtatcagaga ttgttgtggg tgacaaacgt
     1081 atcaaaattg aaatcggcaa gttttccgaa caagtcagcg gcgctgtctt ggccacctgt
     1141 ggtgaaaccg tggttcatag caccgttgcg cttggtcgca aagtcaatct gggctatttt
     1201 cccctctcag ttgaatttgc agaaaaactc tattctgcag gaactattaa aggttcacag
     1261 tgggttaagc gagaaggcag acctctcgat gaggaaatac tcagcgctcg tgtgattgat
     1321 cgtactttac gaccactatt ttctgatggt attaccaatg aagtccaggt aatcaacact
     1381 gttttttcct ttgatggtga aaatgatcca gacatgattg ggctcgttgg cagcgcagtt
     1441 gctttagcag tctctgaaat cccgttcaat ggtcctgttg caggagtgag agttggctat
     1501 cgcaaagaaa gtggtgagtt tcttctaaat ccaactcata ctgaacgcga aaccagtgat
     1561 ttagacttaa ttgtttctgg taccggcgaa accatcgtca tggtagaagc tggcgcgaaa
     1621 gaagttgagg agtcagtggt gattgaagca atgaccaaag ctcaagagca atttgccagt
     1681 atgtgtaaac aaattgccgc tattgccaaa gaagtaggta aggagaaaat caatttagtc
     1741 gaccctgagg cagaagagct cacc
//
LOCUS       DAOQZP010000117         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_91133_length_1643_cov_3.938287, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000117 DAOQZP010000000
VERSION     DAOQZP010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91133_length_1643_cov_3.938287"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PKH60_05385"
     CDS             complement(<1..274)
                     /locus_tag="PKH60_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNQ17502.1"
                     /translation="MKILVVEDEHRIATYIKKGLEIKSHVVDVAYDGESGYDLASTEN
                     YDVLILDRMLPKLDGIAIIRRLRSENNHVPILLLTAKTEVEDRVEGL"
     gene            complement(334..1458)
                     /locus_tag="PKH60_05390"
     CDS             complement(334..1458)
                     /locus_tag="PKH60_05390"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNQ17503.1"
                     /translation="MKNPNHYEPHTVFFDGRDATGKSTLRSIVSRLLTKRGVDVLRQG
                     EFSGYATGTLMRKQIREQRFLSFDGRGTSPEADALILAGDLLLQLKDVVSAKNAGKVA
                     IIDRGPLSFYGYQSARLAATQAESKIICNDESDWLLNLARLLVLPRLTLFLRISDQEM
                     MRRIEARGENPLNANELSYLQTVESIMENNLDLGGQTIFVDAQHDAGITTPLMATLLL
                     QVLSQPTFDLHNTFLTTDEVSVALIKPEFEKLDSTDQASLSNQIRQLTQNTLIGKRTL
                     HLSRSTTGAMWSEDLAKYDWGESYCDFMTTGPVTALVLRGQSSAREMKLTIREQWAPT
                     IQALSTETPFSQDLVHGSDFEDGKLEMDILKLSAQSRLVN"
     gene            complement(1455..>1643)
                     /locus_tag="PKH60_05395"
     CDS             complement(1455..>1643)
                     /locus_tag="PKH60_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNQ17504.1"
                     /translation="PGELFPQYQGVFSYKPGFFPVAEKFFTTAIKIPVWVNSEDRTLV
                     EMYADAIEKVILNKHELL"
BASE COUNT          486 a          340 c          285 g          532 t
ORIGIN      
        1 ccaaaccctc aactctgtct tcaacctctg ttttcgccgt taataataag atagggacgt
       61 gattattttc tgaccgcaac ctacgaataa ttgctatccc atctagcttg ggcagcatac
      121 gatctagaat tagtacgtca tagttttcag ttgatgccaa atcatatcca ctctcgccat
      181 cataggctac atcaaccaca tgagatttta tttctaaacc ttttttaata tacgtcgcaa
      241 tccgatgctc atcttcaaca actagaattt tcattctagt ctattgtatc cccagacttg
      301 agaattaatt gagagaaaat gagagataat aaattagttg accaaacgtg attgtgcact
      361 caatttaaga atatccattt ctaatttacc gtcttcgaaa tcactgccat gcactaaatc
      421 ttggctaaaa ggtgtttcgg ttgaaagcgc ttgaattgtg ggagcccatt gctcacgaat
      481 ggtaagtttc atttctctcg cggaagattg tccacgaaga actaatgctg tgactggtcc
      541 ggtagtcatg aaatcacagt atgattcgcc ccaatcatat tttgctaaat cttctgacca
      601 cattgcgcca gtagtactcc ttgacaagtg taaagtacgt ttgccaataa gtgtattttg
      661 agtcagttgc cgtatttgat ttgaaagaga agcttggtca gttgaatcta atttttcgaa
      721 ttctggtttt atgagtgcaa ccgatacttc atcggtggtt aaaaaagtat tatgtaaatc
      781 aaacgtgggt tggcttaata cctgtaataa aagagtagcc atcaaggggg tcgtaatgcc
      841 cgcatcgtgt tgggcgtcta caaaaatagt ttgacctcct aagtcaagat tattttccat
      901 tattgattca acagtttgta aataactcag ctcattagcg tttagaggat tttcacctct
      961 tgcttcaatc cttctcatca tctcctgatc agaaattctt aggaaaagtg ttaatctggg
     1021 aagtactaat aacctagcca agtttaagag ccaatcgctt tcatcattac aaattatttt
     1081 tgactctgct tgtgtagccg ctagtcttgc agattgatac ccatagaaag agagcggacc
     1141 tctatcgata attgctacct tgccagcatt ttttgcacta actacatctt ttagttgtag
     1201 taataaatcc ccagctaaaa tcaatgcatc tgcctctgga gatgtgcctc tgccgtcaaa
     1261 agaaaggaat ctctgctcac gaatttgttt tctcattaaa gtaccagttg catagccaga
     1321 aaactctcct tgtcttagaa cgtcaacgcc tctttttgtt agaagccgac ttacaatcga
     1381 tcttaaagta gatttaccag tcgcatctct accatcaaaa aaaacagtat gtggttcgta
     1441 atgatttgga tttttcataa taattcgtgt ttattcaaaa ttactttttc aatagcgtct
     1501 gcatacattt cgactaatgt tctatcctct gaattaaccc aaactgggat ctttattgcg
     1561 gtagtaaaaa atttctcagc aaccggaaaa aatccaggtt tataactaaa tacaccctga
     1621 tactgaggaa ataactctcc tgg
//
LOCUS       DAOQZP010000118         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_104721_length_1511_cov_4.090659, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000118 DAOQZP010000000
VERSION     DAOQZP010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104721_length_1511_cov_4.090659"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..1394)
                     /locus_tag="PKH60_05400"
     CDS             complement(141..1394)
                     /locus_tag="PKH60_05400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNQ17505.1"
                     /translation="MQSTKVFMIGWEYPPHNSGGLGVACDGLTRALAAQNTQIYFTLP
                     YAHTGNVQHLSLLGCFDPDWSIDGRLATAPPFGAYQVSSEEMAFNQLAEIVPKLDSTE
                     LRALPQSELDARVNEYATMVAAEEKKVDKQINVVHAHDWMSFPAAMKIRQQSNKPVVA
                     HVHSTEFDRVPHGQGSQFIHQVEYQGLLAATHIIAVSAFTKQLLVNQYSINPNKISVV
                     HNGIDPLNQPKQRAEFAHNRPVIVFMGRLTMQKGGEYFLQLAQRLLNKIPTALFVIAG
                     NGDMYHQLLFKTAFDRLSAHVLFSGFVRDRQRELLLNRADVFVMPSLSEPFGLVALEA
                     AQRHTPVVVSKQSGVAEVLPSAVQVDFWDIDRMVQEISHLIQNSSFARERVQNQLADI
                     SEVTWSTAAGKVRDIYQRLFQKPLR"
BASE COUNT          443 a          320 c          324 g          424 t
ORIGIN      
        1 aaaacagctg ggaccgctta tctacaacca agccatcgaa gatgtgcgta tttggtttgg
       61 acaaaaatta ccctatttgg aggcagattt agatttactg aaaaaagatg catccttggt
      121 ggatgaaaag ctataacaat ttatctgagt ggtttttgaa ataagcgttg ataaatatcg
      181 cgcactttac cagcggcagt tgaccaagtt acttcagaaa tatccgctag ttggttttgc
      241 actctctccc gggcaaaact tgagttttga attaaatgcg aaatttcttg taccattctg
      301 tcaatatccc aaaaatcgac ttgaaccgct gagggcagaa cttctgccac gccagattgt
      361 ttactaacca ccactggcgt atgacgctgg gcagcctcaa gcgctaccaa cccaaagggc
      421 tcagagagtg atggcattac aaatacatcg gcacgattga gcaacagctc tcgctgcctg
      481 tctctcacaa aaccactaaa aaggacatgg gcggataatc gatcaaaagc tgttttgaat
      541 aataattgat gatacatatc accgttacca gcaataacaa acaaagcagt gggaatttta
      601 ttcagcagac gttgagcaag ctgtaaaaag tattccccgc ctttttgcat tgttaaacga
      661 cccataaaca caattactgg tcgattatga gcaaactctg cgcgttgctt tggttggttg
      721 agtggatcaa taccattgtg gacaacacta attttatttg ggttgatcga gtactggtta
      781 acgagtaatt gcttggtaaa tgcactcacg gcgataatgt gagtagcagc cagtaaacct
      841 tgatattcta cttggtgaat aaattgactg ccttgaccat gtgggaccct atcgaattcg
      901 gtcgagtgaa catgggcgac aactggtttg tttgattgtt ggcgaatttt catggccgca
      961 ggaaaagaca tccagtcatg tgcatgaacc acattaattt gtttatcaac tttcttttcc
     1021 tccgcagcca ccatcgtcgc atattcattg actcgcgcat caagttcaga ttgtggcagt
     1081 gctctgagtt cagtggagtc aagttttggt acgatttcag ctagttgatt gaatgccatc
     1141 tcttcagaac ttacttgata tgcgccaaac gggggagctg ttgcaagccg tccatctatg
     1201 gaccaatctg gatcaaaaca accaagtagg gagaggtgtt ggacattccc ggtatgagcg
     1261 tatggcaaag taaaataaat ttgggtattt tgagccgcta gcgctctggt taagccgtcg
     1321 caggcaacac caagacctcc actattatgt ggagggtatt cccaaccaat cataaacact
     1381 tttgttgatt gcatcgacat accaaaccaa aaagctacta cggttagctt ttaaatgtac
     1441 tcgctcgtat atacgcttca tccagtgtac aagataacaa attaccattg caagcagtaa
     1501 tttatgatta t
//
LOCUS       DAOQZP010000119         1501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_0981 NODE_105862_length_1501_cov_5.154910, whole genome
            shotgun sequence.
ACCESSION   DAOQZP010000119 DAOQZP010000000
VERSION     DAOQZP010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852324
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:55:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,081
            CDSs (total)                      :: 1,045
            Genes (coding)                    :: 1,043
            CDSs (with protein)               :: 1,043
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105862_length_1501_cov_5.154910"
                     /isolate="AMR_MDS_0981"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /locus_tag="PKH60_05405"
     CDS             complement(<1..1439)
                     /locus_tag="PKH60_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HNQ17506.1"
                     /translation="MAKKQINPHFVAILKEIESTPNCTAKQLRQILGKFAPASGGLYS
                     KTVLLAAYRELAGTHGLQPFSDKVALLLRKKPVRTLSGVAPITVLSKPFPCPGKCIFC
                     PNDIRMPKSYLADEPGAQRAERNWFDPYLQTLTRLQALHNLGHPVDKAEIIILGGTWS
                     DYPEPYQIWFVKECFRALNEFGIRDDRDRIRTQYLEMKQTYRQNGGVGFTNDPTENEK
                     LVENKQIHGEYLEKSYNQVISELYVAPERKAGFDTYQSATWEELIEQQRINETGKIRC
                     VGLVVETRPDNISENEVIRIRKLGATKVQIGVQSLQDEVLTLNKRGHDVAATRRAFKL
                     LRSMGFKIHAHWMPNLYGSTVEKDKADFLRLFDDPDFCPDELKLYPCSLIESAELMQQ
                     YQAGTWQPYTREELLEVLVFAVSHTPEYCRLTRIVRDIPSTDIVVGNKTTNFREVVDA
                     SMIKSKVKSKDIRAREVKKQELDPSTLVL"
BASE COUNT          395 a          352 c          294 g          460 t
ORIGIN      
        1 ctcaaaacta gcgtgcttgg gtcaagttct tgtttcttga cttctctggc acgaatatct
       61 ttgcttttca ccttcgattt aatcatgcta gcatcaacca cttcgcgaaa attcgtagtt
      121 ttattgccaa ccacaatgtc agttgaggga atatccctca caattcttgt caaacgacaa
      181 tattcgggcg tgtgactcac tgcaaaaacc aaaacctcaa gcaattcttc tcttgtgtaa
      241 ggttgccaag tgcctgcttg atactgctgc attaattcag cactttcgat taaagaacag
      301 ggatataact ttaactcgtc ggggcaaaag tctggatcgt cgaatagtct taaaaagtca
      361 gctttgtctt tttccactgt cgaaccgtac aaattgggca tccaatgagc gtgaattttg
      421 aatcccatcg aacgaagtaa cttaaaagct cgtcttgtcg cggccacgtc gtggccacgc
      481 ttgttaagag tcaacacctc gtcctgcaaa ctttgcaccc cgatctgaac ctttgtggct
      541 ccaagcttcc taattctgat cacttcgttc tcagatatat tatctggtct agtttcaaca
      601 actaaaccaa cgcaacgaat cttgccagtc tcattgattc gctgttgttc aattagttcc
      661 tcccaagtcg ctgattggta cgtatcaaat cccgcttttc tctctggtgc tacatagagt
      721 tcggaaatca cctggttgta tgatttttcg aggtactcac catgaatttg tttattctca
      781 actagttttt cattttcagt tggatcattg gtaaaaccta cgccaccatt ttgccgatat
      841 gtttgcttca tctctaaata ctgagttcta attctgtcac gatcgtctcg aataccaaac
      901 tcattaagcg cacgaaagca ctctttcaca aaccaaattt gatatggctc aggatagtca
      961 gaccaagtac cacctaaaat gataatctct gctttgtcta cagggtggcc cagattgtgc
     1021 agagcttgta gacgagtgag tgtttgcaaa tagggatcaa accaattacg ctcagctcgc
     1081 tgagcgcccg gctcatctgc taaataactt tttggcatac gtatgtcatt tggacaaaaa
     1141 atacacttgc ccgggcatgg aaatggcttt gataaaaccg taattggcgc tactccagaa
     1201 agggttcgaa ctggcttttt cctgagcaac aaagcaactt tgtcagaaaa tggctgaagt
     1261 ccgtgggtgc ccgccaattc tcggtatgct gcaagaagaa cggttttact gtataaaccg
     1321 ccacttgcgg gcgcaaactt ccccagtatt tgtcgcaact gtttggctgt gcagtttggc
     1381 gttgactcaa tttctttcaa gatagccaca aaatgtggat tgatctgttt ttttgccata
     1441 ggtgatttat actgtgggta ttctacctgt ttgaagccaa aatacgcatg caaactacca
     1501 c
//
